tbuckley/cabot-search huggingface dataset:
Effects of CFTR, interleukin-10, and Pseudomonas aeruginosa on gene expression profiles in a CF bronchial epithelial cell Line
Authors: Isabel Virella-Lowell, John-David Herlihy, Barry Liu, Cecilia Lopez, Pedro Cruz, Chris Muller, Henry V. Baker, Terence R. Flotte
Journal: Molecular Therapy (2004)

Mutations in CFTR lead to a complex phenotype that includes increased susceptibility to Pseudomonas infections, a functional deficiency of IL-10, and an exaggerated proinflammatory cytokine response. We examined the effects of CFTR gene correction on the gene expression profile of a CF bronchial epithelial cell line (IB3-1) and determined which CF-related gene expression changes could be reversed by IL-10 expression. We performed microarray experiments to monitor the gene expression profile of three cell lines over a time course of exposure to Pseudomonas. At baseline, we identified 843 genes with statistically different levels of expression in CFTR-corrected (S9) cells compared to the IB3-1 line or the IL-10-expressing line. K-means clustering and functional group analysis revealed a primary up-regulation of ubiquitination enzymes and TNF pathway components and a primary down-regulation of protease inhibitors and protein glycosylation enzymes in CF. Key gene expression changes were confirmed by real-time RT-PCR. Massive reprogramming of gene expression occurred 3 h after Pseudomonas exposure. Changes specific to CF included exaggerated activation of cytokines, blunted activation of anti-proteases, and repression of protein glycosylation enzymes. In conclusion, the CFTR genotype changes the expression of multiple genes at baseline and in response to bacterial challenge, and only a subset of these changes is secondary to IL-10 deficiency.
The oncoprotein gankyrin binds to MDM2/HDM2, enhancing ubiquitylation and degradation of p53
Authors: Hiroaki Higashitsuji, Hisako Higashitsuji, Katsuhiko Itoh, Toshiharu Sakurai, Toshikazu Nagao, Haruhiko Sumitomo, Tomoko Masuda, Simon Dawson, Yutaka Shimada, R. John Mayer, Jun Fujita
Journal: Cancer Cell (2005)
Leber's Hereditary Optic Neuropathy Is Associated with the T12338C Mutation in Mitochondrial ND5 Gene in Six Han Chinese Families
Authors: Xiao-Ling Liu, Xiangtian Zhou, Jian Zhou, Fuxin Zhao, Juanjuan Zhang, Chengwu Li, Yanchun Ji, Yu Zhang, Qi-Ping Wei, Yan-Hong Sun, Li Yang, Bing Lin, Yumin Yuan, Yingzi Li, Jia Qu, Min-Xin Guan
Journal: Ophthalmology (2010)
Mechanical properties of human forearm and vulvar skin
Authors: P. ELSNER, DOROTHEA WILHELM, H.I. MAIBACH
Journal: British Journal of Dermatology (1990)

Journal Article Mechanical properties of human forearm and vulvar skin Get access P. ELSNER, P. ELSNER University of Würzburg, Federal Republic of Germany Search for other works by this author on: Oxford Academic Google Scholar DOROTHEA WILHELM, DOROTHEA WILHELM Department of Dermatology, School of Medicine, University of California San Francisco Search for other works by this author on: Oxford Academic Google Scholar H.I. MAIBACH H.I. MAIBACH Department of Dermatology, School of Medicine, University of California San Francisco Professor H.I. Maibach, Department of Dermatology, University of California School of Medicine, Box 0989, Surge 110, San Francisco, CA 94143–0989, U.S.A. Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 122, Issue 5, 1 May 1990, Pages 607–614, https://doi.org/10.1111/j.1365-2133.1990.tb07282.x Published: 01 May 1990
TCT-728 The Impact of FDA Approval of Transcatheter Aortic Valve Replacement on the Treatment Assignment of Patients with Severe Aortic Stenosis
Authors: Ricardo O. Escarcega, Rebecca Torguson, Joelle Salmon, Sandeep Kumar, Marcus Weaver, Nevin C. Baker, Marco A. Magalhaes, Michael J. Lipinski, Sa'ar Minha, Fang Chen, Thibault Lhermusier, lakshmana Pendyala, Wenjie Tian, Hideaki Ota, William O. Suddath, Lowell F. Satler, Augusto Pichard, Ron Waksman
Journal: Journal of the American College of Cardiology (2014)
Ultrasensitive Clinical Enumeration of Rare Cells ex Vivo Using a Micro-Hall Detector
Authors: David Issadore, Jaehoon Chung, Huilin Shao, Monty Liong, Arezou A. Ghazani, Cesar M. Castro, Ralph Weissleder, Hakho Lee
Journal: Science Translational Medicine (2012)

A hybrid microfluidic/semiconductor chip analyzes single, immunomagnetically tagged ovarian cancer cells in unprocessed biological samples.
Twin disulfides as opportunity for improving stability and transfection efficiency of oligoaminoethane polyplexes
Authors: Philipp M. Klein, Katharina Müller, Christina Gutmann, Petra Kos, Ana Krhac Levacic, Daniel Edinger, Miriam Höhn, Jean-Christophe Leroux, Marc A. Gauthier, Ernst Wagner
Journal: Journal of Controlled Release (2014)
Establishing a Therapeutic Range for Heparin Therapy
Authors: Patrick Brill-Edwards
Journal: Annals of Internal Medicine (1993)

Objective: To compare two methods of determining a therapeutic range of activated partial thromboplastin time (aPT) results. Design: Cohort studies. Setting: Referral teaching hospital. Patients: Inpatients who received unfractionated heparin intravenously for venous thromboembolic disease. Measurements: A therapeutic range determined by aPT ratios of 1.5 to 2.5 times the control value as compared with a therapeutic range determined by protamine titration heparin levels of 0.2 to 0.4 U/mL. Results: For all aPT reagents studied, a ratio of 1.5 times the control value is much less than a minimum protamine titration heparin level of 0.2 U/mL. Various manufacturers' aPT reagents and reagent lots from the same manufacturer show considerable variation in response to heparin and therefore have different therapeutic ranges. Conclusions: A different dose of heparin would be required to produce an aPT ratio of 1.5 times the control value, depending on the reagent used. Establishing a therapeutic range for aPT results using protamine titration heparin levels of 0.2 to 0.4 U/mL as a reference standard is practical and compensates for the variable response of aPT reagents to heparin.
More on the Declining Age at HIV Infection
Authors: T.H. Hughes-Davies
Journal: New England Journal of Medicine (1994)

To the Editor: The chance of, and so the delay in, encountering a condition depends on its prevalence. A new doctor was likely to wait longer to see his first case of AIDS in 1983 than in 1993. Similarly, a patient is likely to meet the human immunodeficiency virus after fewer exposures now than 15 years ago. The decline in the age at infection that Dr. Rosenberg and his colleagues (March 17 issue)1 describe was to be expected.
Pregnancy and Chronic Progressive Pulmonary Disease
Authors: Isaiah D. Wexler, Marie Johannesson, Frank P. Edenborough, Beth S. Sufian, Eitan Kerem
Journal: American Journal of Respiratory and Critical Care Medicine (2006)

Progressive pulmonary disease may preclude the option of pregnancy for a number of women in their child-bearing years due to the severity of the disease. For a subset of women with chronic lung disease including cystic fibrosis, pregnancy is possible, but can have a devastating effect both on the prospective mother and fetus. The potential hazards of pregnancy in cystic fibrosis or other progressive pulmonary diseases may trigger a moral conflict between physician and patient. The female patient may argue that her autonomy cannot be circumscribed and that the physician is obliged to assist her reproductive efforts. The physician can counter that his/her participation in potentially harmful interventions is not consistent with professional norms requiring adherence to the principles of beneficence and nonmaleficence. Whenever possible, the ethical conflict between physician and patient should be resolved before initiation of pregnancy. We propose that this best be done through structured negotiations between physician and patient with the goal of constructing an ethical framework for reducing the moral tension between the two. Steps in the negotiating process include defining the therapeutic alliance, information exchange, dialog, and deliberation. As part of the information exchange, it is important to discuss alternatives to pregnancy such as adoption and surrogacy, especially when there are strong contraindications to pregnancy. If negotiations reach a satisfactory conclusion for both sides, there should be a well-delineated consensual agreement to commence the pregnancy with the full support of the medical team.
No association of the NFKB1 promoter polymorphism with ulcerative colitis in a British case control cohort
Authors: M M Mirza
Journal: Gut (2005)

Management of acute pancreatitisNo account of the complications of acute pancreatitis (Gut 2005;54:426-36) would be complete without mention of diabetic ketoacidosis as an association, which is either fortuitous or one which exists as a complication in its own right.Recognition of this association has been inhibited by the complicated relationship between diabetic ketoacidosis, acute abdominal pain, and hyperamylassaemia, notwithstanding the
Linkage analysis of Dermatophagoides pteronyssinus–specific IgE responsiveness with polymorphic markers on chromosome 6p21 (HLA-D region) in Caucasian families by the transmission/disequilibrium test
Authors: Nobuyuki Hizawa, Gary Collins, Thorunn Rafnar, Shau-Ku Huang, David L. Duffy, James L. Weber, Linda R. Freidhoff, Eva Ehrlich, David G. Marsh, Terri H. Beaty, Kathleen C. Barnes
Journal: Journal of Allergy and Clinical Immunology (1998)

Background: Recently, we have obtained evidence for linkage between Der p 1–specific IgE antibodies and markers on chromosome 6p21 (HLA-D region) in a genome-wide screening in Caucasian families recruited as a part of the Collaborative Study on the Genetics of Asthma (CSGA).Objective: Specific IgE antibodies toward different Dermatophagoides pteronyssinus (Der p) polypeptides were detected by immunoblotting analysis, and the transmission/disequilibrium test (TDT) was performed between specific IgE responsiveness toward each different Der p polypeptide and markers on chromosome 6p21 to better clarify the genetic contribution of HLA-D genes.Methods: We studied 299 individuals in 45 Caucasian families participating in the CSGA. Serum samples from 137 individuals that showed elevated specific IgE antibodies toward the Der p crude allergen (> –0.5 log IU/mL) by ACCESS immunoassay were subjected to immunoblotting analysis. TDT was conducted between the presence of specific IgE antibodies toward each of 12 different Der p polypeptides and 4 polymorphic markers on chromosome 6p21.Results: The 196-bp allele of D6S1281 and the 104-bp allele of DQCAR showed significant excess transmission to specific IgE responders toward a particular Der p polypeptide (120 kd, 55 kd, 45 kd, or 37 kd). In contrast, the 200-bp allele of D6S1281 and the 204-bp allele of D6S291 showed significantly decreased transmission to specific IgE responders toward a particular Der p polypeptide (120 kd, 90 kd, 52 kd, or 45 kd). Deviation from the expected 50% transmission in heterozygous parents was statistically significant after correcting for multiple comparisons.Conclusion: This study supported our previous findings that genes on chromosome 6p21 (HLA-D region) may influence the expression of Der p–specific IgE responsiveness in this Caucasian population. Our results, however, reveal the complexity of genetic regulations of Der p–specific IgE responsiveness by HLA-D genes, suggesting the strong influence of non-HLA loci and perhaps environmental factors for the development of Der p–specific IgE responsiveness. (J Allergy Clin Immunol 1998;102:443-8.)
DIAGNOSIS IN ORTHOPEDIC SURGERY
Authors: FRANK R. OBER
Journal: JAMA (1926)

The orthopedic surgeon of today is well trained in the application of therapeutic measures to prevent and relieve deformity, but he is often faced with problems which have not been properly solved or which have been solved only after considerable injury has been done to important structures and he is expected to do the impossible in the restoration of function. Some of the reasons for this are wrong  late  snap diagnosis or the diagnosis of hysteria.  Musser 1 says, Success in treatment requires both accuracy and completeness in diagnosis, and Partial knowledge of a case differs in degree only from ignorance. To my mind these two sayings are as true today as they were twenty-five years ago, and they should make us pause and ask ourselves whether or not we are endeavoring to do everything in our power to locate and discover the trouble with many of our
A Rapid Test for Sickle Hemoglobin
Authors: Toshio Asakura
Journal: JAMA (1975)

Vigorous shaking of oxygenated sickle hemoglobin (Hb S) in solution causes precipitation of the hemoglobin. This unusual property of Hb S forms the basis of a simple test for the presence of Hb S. Comparison of results of this test with those from cellulose acetate electrophoresis in blood samples from 599 patients showed no false-positive or false-negative results. The group tested included 55 persons with sickling disorders and 47 others with unusual hemoglobin patterns. This test may be used to confirm the presence of Hb S or to establish the diagnosis of sickling disorders rapidly in the clinic or at the bedside. (<i>JAMA</i>233:156-157, 1975)
The Vienna Anglo-American Medical Association.
Authors: F. G. Harris
Journal: JAMA (1904)

Vienna, March 29, 1904. <h3>To the Editor:</h3> —The Anglo-American Medical Association of Vienna was organized Feb. 6, 1904, with 56 members, and now has a membership of 80. The object of the organization, as stated in the constitution, is to promote social intercourse among the English-speaking medical men studying in Vienna, to advance the scientific interests of its members and to aid in quickly acquainting the newcomer with the medical and scientific courses given in Vienna. The association meets every other Saturday evening for supper, after which there is a scientific and social meeting, the former consisting of an address on some medical topic by one of the professors or teachers connected with the university. Professors Ortner and Finger and Private-Docent Sternberg have delivered very interesting and instructive addresses. Inquiries addressed to the secretary, Dr. F. G. Harris, 8 Schlösselgasse No. 2, Vienna, will be cheerfully answered. [The officers of
The excimer laser: Gross, light microscopic and ultrastructural analysis of potential advantages for use in laser therapy of cardiovascular disease
Authors: Jeffrey M. Isner, Roberta Fortin Donaldson, Lawrence I. Deckelbaum, Richard H. Clarke, Sylvie M. Laliberte, Angelo A. Ucciz, Deeb N. Salem, Marvin A. Konstam
Journal: Journal of the American College of Cardiology (1985)
HYPERSENSITIVENESS TO SOLUBLE SPECIFIC SUBSTANCES FROM YEAST-LIKE FUNGI
Authors: H. D. Kesten, E. Mott
Journal: The Journal of Experimental Medicine (1931)

1. The polysaccharide fractions from each of five yeast-like fungi produce rapid, fatal anaphylactic shock in guinea pigs passively sensitized with antiserum from rabbits immunized against the killed organisms. 2. Cross-anaphylactic reactions with heterologous polysaccharide fractions are frequent. They parallel closely the cross-precipitin reactions, thus adding evidence in favor of the identity of precipitin and sensitizing antibody. 3. The polysaccharide fraction from Monilia psilosis produces anaphylactic death in guinea pigs actively sensitized with killed homologous organisms, but an attempt to sensitize actively with the polysaccharide fraction was unsuccessful.
Human HMGA2 protein overexpressed in mice induces precursor T-cell lymphoblastic leukemia
Authors: A Efanov, N Zanesi, V Coppola, G Nuovo, B Bolon, D Wernicle-Jameson, A Lagana, A Hansjuerg, F Pichiorri, C M Croce
Journal: Blood Cancer Journal (2014)

Abstract T-cell acute lymphoblastic leukemia (T-ALL) is a neoplasia of thymocytes characterized by the rapid accumulation of the precursors of T lymphocytes. HMGA2 (high-mobility group AT-hook 2) gene expression is extremely low in normal adult tissues, but it is overexpressed in many tumors. To identify the biological function of HMGA2, we generated transgenic mice carrying the human HMGA2 gene under control of the V H promoter/Eμ enhancer. Approximately 90% of Eμ- HMGA2 transgenic mice became visibly sick between 4 and 8 months due to the onset and progression of a T-ALL-like disease. Characteristic features included severe alopecia (30% of mice); enlarged lymph nodes and spleen; and profound immunological abnormalities (altered cytokine levels, hypoimmunoglobulinemia) leading to reduced immune responsiveness. Immunophenotyping showed accumulation of CD5+CD4+, CD5+CD8+ or CD5+CD8+CD4+ T-cell populations in the spleens and bone marrow of sick animals. These findings show that HMGA2-driven leukemia in mice closely resembles spontaneous human T-ALL, indicating that HMGA2 transgenic mice should serve as an important model for investigating basic mechanisms and potential new therapies of relevance to human T-ALL.
Mesenchymal stromal cells form vascular tubes when placed in fibrin sealant and accelerate wound healing in vivo
Authors: Julio J. Mendez, Mahboobe Ghaedi, Amogh Sivarapatna, Sashka Dimitrievska, Zhen Shao, Chinedum O. Osuji, Derek M. Steinbacher, David J. Leffell, Laura E. Niklason
Journal: Biomaterials (2014)
Impact of Down syndrome on the performance of neonatal screening assays for severe primary immunodeficiency diseases
Authors: Ruud H.J. Verstegen, Stephan Borte, Levinus A. Bok, Paul H.Th. van Zwieten, Ulrika von Döbeln, Lennart Hammarström, Esther de Vries
Journal: Journal of Allergy and Clinical Immunology (2013)
NEUTRON ACTIVATION ANALYSIS OF TRACE ELEMENTS IN SKIN.
Authors: MOHAMED MOLOKHIA, BENJAMIN PORTNON
Journal: British Journal of Dermatology (1969)

SUMMARY.— Manganese levels in normal epidermis and dermis from 34 cadavers were estimated by neutron activation analysis. The mean concentration of manganese in the epidermis was 4 times higher than that in the dermis. An important biological role of manganese in skin is suggested by the remarkably narrow range of concentration of that trace metal in the analysed samples.
Direct recordings of the temperatures in the tracheobronchial tree in normal man.
Authors: E R McFadden, D M Denison, J F Waller, B Assoufi, A Peacock, T Sopwith
Journal: Journal of Clinical Investigation (1982)

In an effect to determine how far inspired air could penetrate into the respiratory tract before being brought to body conditions, we measured the temperature in the airways of the anterior basilar segment of the right lower lobe in five normal subjects while they breathed air at subfreezing and ambient conditions. During quiet breathing, most of the heating of the incoming gas took place in the upper airways as expected. However, as the thermal burden was increased by rapid inspirations, frigid air, and hyperventilation, the temperature of the distal airways progressively fell and the point at which the incoming air reached body conditions moved deep into the periphery of the lung. These findings demonstrate that heat and water transfer is not localized to one region, but rather is a continuous process that begins the moment the air enters the body and involves as much of the respiratory tract as necessary to complete the task.
Matrix metalloproteases in bronchoalveolar lavage fluid of patients with type III Pseudomonas aeruginosa pneumonia
Authors: Ali A. El-Solh, Daniel Amsterdam, Ahmad Alhajhusain, Morohonfolu E. Akinnusi, Ranime G. Saliba, Susan V. Lynch, Jeanine P. Wiener-Kronish
Journal: Journal of Infection (2009)
CASE OF (SYPHILITIC?) ULCER OF THE BLADDER
Authors: IRVING SIMONS
Journal: JAMA (1913)

On account of the rarity of ulcer of the bladder, in behalf of its successful treatment, and lastly, on account of some unique features in this particular case, it occurred to me that it might be worth recording. <h3>History.</h3> —The patient, P. J., male, aged 24, house-painter, married, denies gonorrhea and lues. He has three healthy children; all have come under observation, and have been thoroughly examined. Wife has had no miscarriages. For almost two years he has had hypogastric pain, dull and dragging in character; it was at first intermittent; but now has become practically continuous day and night. Pain increases as soon as the bladder becomes even moderately distended with urine, so that he has been voiding about every hour daily, and during the last six months about five or six times at night. He experieces relief after emptying the bladder. There is no loin pain, or pain
Traumatic Amputation of Upper Limb
Authors: G. R. C. Peatfield
Journal: BMJ (1950)

~~~~~~~~~~~~~~~~~~~~~~~MEDICAL JouwNAIexternal genitalia are duplicated too, whilst others use it in cases in which duplication is incomplete.The association of congenital abnormalities in other systems with defects in the genital tract has been commented on by many authors and was recently emphasized by Burton-Brown (1948).In particular, various develop- mental errors are to be found in the urinary organs-a fact which is not surprising, bearing in mind that the genital and urinary organs are so closely associated in their development.
A URETERAL STONE DISLODGER
Authors: Robert W. McKay
Journal: JAMA (1930)

A number of ureteral stone dislodgers and extractors have been devised in the past few years. In the majority of these, the device that traps the stone has made the caliber of the whole instrument so great that it is passed by the obstructing calculus with difficulty. To secure an instrument with the smallest diameter possible, I designed the dislodger described here: It consists of a number 1 or a 2 bougie. One and one-half inches from the tip of the bougie there are attached four stout threads of Japanese silk (tooth silk). These are made fast to the bougie core by very fine silk threads wrapped about their ends, as shown in figure 1. This attachment of the threads to the core marks the greatest diameter in the entire instrument. The point of attachment is spindle shaped so as to pass by the stone with the maximum of ease.