tbuckley/cabot-search huggingface dataset:
THE INTERACTION OF THYROXINE WITH PLASMA PROTEINS: LOCALIZATION OF THYROXINE-BINDING PROTEIN IN COHN FRACTIONS OF PLASMA 12
Authors: Norbert Freinkel, J. Thomas Dowling, Sidney H. Ingbar
Journal: Journal of Clinical Investigation (1955)

Recently zone electrophoretic techniques have been employed to characterize the site of thyrox- ine-binding in plasma (1-7).In most reports, an association has been described between thyroxine and a protein moiety intermediate in electrophoretic mobility at pH 8.6 between the al and the a, globulins.A concentrated source of this thyroxine-binding protein (TBP) has not been available for further purification or for experimental evaluation of its physiological role.To meet these objectives, zone electrophoretic techniques have been employed to localize TBP in subfractions of plasma prepared by Method 6 of Cohn and his co-workers (8, 9).MATERIALS 1. Cohn Fractions: Fractions were obtained from 250 milliliters of fresh plasma and from 100 liters of plasma derived from out-dated blood-bank blood.Two sepa- rate fractionations were performed with ethanol-water mixtures of controlled pH, ionic strength, temperature, and protein concentration.Yields from the large-scale fractionation of old plasma were sufficient to permit char- acterization of the individual fractions by moving-boundary electrophoresis and by chemical estimation of iodine/ nitrogen ratios (Table I).2. Thyroxine: Chromatographically-pure I1"-labelled thyroxine (10), dissolved in propylene glycol was ob- tained from a commercial source.
Small molecule fluorophore and copolymer RGD peptide conjugates for ex vivo two-photon fluorescence tumor vasculature imaging
Authors: Alma R. Morales, Ciceron O. Yanez, Yuanwei Zhang, Xuhua Wang, Sanchita Biswas, Takeo Urakami, Masanobu Komatsu, Kevin D. Belfield
Journal: Biomaterials (2012)
Elevated serum iron indices in haptoglobin phenotype 2-2 individuals being homozygous for the HFE missense mutation
Authors: Hans Van Vlierberghe, Michel Langlois, Joris Delanghe, Yves Horsmans, Peter Michielsen, Jean Henrion, Rene Cartuyvels, Johan Billiet, Martine De Vos, Geert Leroux-Roels
Journal: Journal of Hepatology (2002)
Intramyocardial Injections and Protection Against Myocardial Ischemia
Authors: Peter Whittaker, Robert A. Kloner, Karin Przyklenk
Journal: Circulation (1996)

Although adenosine has been proposed to be a cardioprotective agent, direct examination of such protection is confounded by its short half-life and hemodynamic effects. We attempted to avoid these problems by injecting adenosine directly into cardiac muscle.We gave four adenosine injections (each 0.15 mL, 5 mg.mL-1 saline) into the left ventricular wall of rat hearts before a 60-minute occlusion. Although infarcts were smaller in adenosine-treated hearts (29 +/- 6%) than in controls (52 +/- 5%; P < .05), injection of saline also reduced infarct size (29 +/- 7%). Infarcts in hearts subjected to needle insertion but no fluid injection differed neither from control nor from fluid-treated hearts (38 +/- 4%). Adenosine reduced ectopic beats and the incidence of ventricular tachycardia during occlusion. In contrast, saline injection prolonged the duration of arrhythmias. To examine the spatial relationship between protection and the injection site, we gave 18 saline injections (each 0.15 mL) into canine myocardium before a 60-minute occlusion. Infarcts were smaller in saline-treated hearts than in controls (P < .01). Because infarcts in four hearts occupied < 3% of the risk region, we concluded that fluid injection did not itself cause appreciable necrosis and speculated that muscle was protected in the vicinity of the injection site. Previous work indicated that muscle can be protected by stretch. We examined this hypothesis by adding gadolinium chloride (a stretch-activated channel blocker) to the saline (0.008 g.mL-1) injection in rat hearts. We again found small infarcts after saline injection (26 +/- 5%); however, gadolinium blocked protection (50 +/- 7%; P < .03).Although we were only partially successful in documenting adenosine-mediated cardioprotection, we found evidence for myocyte protection via a stretch-activated mechanism.
Organizational Factors Affecting the Adoption of Diabetes Care Management Processes in Physician Organizations
Authors: Rui Li, Jodi Simon, Thomas Bodenheimer, Robin R. Gillies, Lawrence Casalino, Julie Schmittdiel, Stephen M. Shortell
Journal: Diabetes Care (2004)

OBJECTIVE—To describe the extent of adoption of diabetes care management processes in physician organizations in the U.S. and to investigate the organizational factors that affect the adoption of diabetes care management processes. RESEARCH DESIGN AND METHODS—Data are derived from the National Survey of Physician Organizations and the Management of Chronic Illness, conducted in 2000–2001. A total of 1,104 of the 1,590 physician organizations identified responded to the survey. The extent of adoption of four diabetes care management processes is measured by an index consisting of the organization’s use of diabetic patient registries, clinical practice guidelines, case management, and physician feedback. The ordinary least-squares model is used to determine the association of organizational characteristics with the adoption of diabetes care management processes in physician organizations. A logistic regression model is used to determine the association of organizational characteristics with the adoption of individual diabetes care management processes. RESULTS—Of the 987 physician organizations studied that treat patients with diabetes, 48% either do not use any or use only one of the four diabetes care management processes. A total of 20% use two care management processes, and 32% use three or four processes. External incentives to improve quality, computerized clinical information systems, and ownership by hospitals or health maintenance organizations are strongly associated with the diabetes care management index and the adoption of individual diabetes care management processes. CONCLUSIONS—Policies to encourage external incentives to improve quality and to facilitate the adoption of computerized clinical information technology may promote greater use of diabetes care management processes.
Treatment of low-risk myelodysplastic syndromes with high-dose daily oral cholecalciferol (2000–4000 IU vitamin D3)
Authors: I Molnár, N Stark, J Lovato, B L Powell, J Cruz, D D Hurd, J S Mathieu, T C Chen, M F Holick, S Cambra, R P McQuellon, G G Schwartz
Journal: Leukemia (2007)
P174Extended preservation of arterial conduits in GALA: multiphoton microscopic evaluation
Authors: HS Thatte, M Haime, MD Crittenden
Journal: Cardiovascular Research (2014)

Purpose: Injury to endothelium can compromise patency of bypass conduits and adversely affect long-term outcomes. We have previously shown that GALA, a synergistic physiological salt solution of our design, that provides energy and nitric oxide substrate, free radical scavenger and reducing agent, significantly preserves endothelial and smooth muscle cell (SMC) function in human saphenous vein (HSV) during ex vivo storage, resulting in improved outcomes. We evaluate the role of GALA in preservation of endothelium and SMC in arterial conduits (radial artery, RA; internal mammary artery, IMA), during short and long-term storage. We hope that as in HSV, successful preservation of viable conduits may improve patient outcomes and prove beneficial, especially for conduit compromised revascularization patients.
116 ALTERED SURFACE EXPRESSION OF INHIBITORY KIR2DL3 AND ACTIVATING CD160, NKG2D RECEPTORS ON NK AND CYTOTOXIC T CELLS IN CHRONIC HCV HEPATITIS
Authors: G. Par, L. Szereday, T. Berki, M. Halasz, A. Miseta, J. Szekeres-Bartho, G. Hegedus, G.Y. Mozsik, B. Hunyady, A. Par
Journal: Journal of Hepatology (2008)
Medical Care of Cambodian Refugees
Authors: Keith Dahlberg
Journal: JAMA (1980)

Tens of thousands of Cambodian refugees are entering Thailand. Many of the new arrivals are survivors of months of starvation and are critically ill with marasmus, kwashiorkor, beriberi, anemia, malaria, diarrhea, and respiratory diseases. With volunteer medical help, field hospitals are treating patients under primitive conditions that are gradually improving. Based on experience at the Sa Kaeo refugee camp, a brief survey of the nutritional and other diseases likely to be encountered is given for the potential volunteer who may be unfamiliar with tropical medicine. (<i>JAMA</i>243:1062-1065, 1980)
Detection of human papillomavirus DNA in colorectal carcinomas by polymerase chain reaction.
Authors: J Y Cheng, L F Sheu, C L Meng, W H Lee, J C Lin
Journal: Gut (1995)

Human papillomaviruses (HPVs) are associated with a number of benign and malignant neoplasms. To substantiate the relationship between HPV DNA and colorectal carcinomas, 70 carcinomas and 37 adenomas were analysed in this study. Specific types of HPV DNA in colorectal tumours were detected by polymerase chain reaction (PCR) and Southern blot hybridisation. HPV DNA was detected in 11 of 37 (29.7%) adenomas and in 52.9% 37 of 70 (52.9%) of carcinomas. The expression of HPV DNA in adenomas and carcinomas, especially that of HPV 16 in HPV positive cases (4 of 11 v 26 of 37), was significantly different (p &lt; 0.05). There was no correlation, however, between HPV and the location, differentiation, stage, or survival of malignant neoplasms. These data suggest that HPV DNA, especially type 16, is associated with colorectal carcinogenesis.
PROGNOSTIC SIGNIFICANCE OF CARDIOVASCULAR MAGNETIC RESONANCE FEATURE TRACKING DERIVED CIRCUMFERENTIAL STRAIN IN CHILDREN UNDERGOING FAMILY SCREENING AND PAEDIATRIC PATIENTS WITH SUSPECTED CARDIOMYOPATHY
Authors: Joanna Petryka, Sadia Quyam, Benjamin Smith, Claire Raphael, Bethan Cowley, Anna N. Seale, Dudley Pennell, Piers Daubeney, Sanjay Prasad
Journal: Journal of the American College of Cardiology (2014)
Elevated Risk of Prostate Cancer Among Men With Lynch Syndrome
Authors: Victoria M. Raymond, Bhramar Mukherjee, Fei Wang, Shu-Chen Huang, Elena M. Stoffel, Fay Kastrinos, Sapna Syngal, Kathleen A. Cooney, Stephen B. Gruber
Journal: Journal of Clinical Oncology (2013)

Prostate cancer has been described as a component tumor of Lynch syndrome (LS), with tumors obtained from mutation carriers demonstrating the DNA mismatch repair deficiency phenotype. Previous studies quantifying prostate cancer risk in LS have provided conflicting results.We examined cancer histories of probands and their first- through fourth-degree relatives for 198 independent mutation-positive LS families enrolled in two US familial cancer registries. Modified segregation analysis was used to calculate age-specific cumulative risk or penetrance estimates, with accompanying Wald-type CIs. Cumulative lifetime risks and hazard ratio (HR) estimates for prostate cancer were calculated and compared with those of the general population.Ninety-seven cases of prostate cancer were observed in 4,127 men. Median age at prostate cancer diagnosis was 65 years (range, 38 to 89 years), with 11.53% of affected individuals diagnosed before age 50 years. The cumulative risk of prostate cancer at ages 60 and 80 years was 6.30% (95% CI, 2.47 to 9.96) and 30.0% (95% CI, 16.54 to 41.30), as compared with the population risk of 2.59% and 17.84%, respectively. The overall prostate cancer HR among carriers was 1.99 (95% CI, 1.31 to 3.03).The cumulative lifetime risk of prostate cancer in individuals with LS is two-fold higher than in the general population and is slightly higher in carriers diagnosed before age 60 years (HR, 2.48; 95% CI, 1.34 to 4.59). These estimates are clinically valuable to quantify risk for both patients and providers.
Narrow-Band Imaging: Potential and Limitations
Authors: K. Kuznetsov, R. Lambert, J.-F. Rey
Journal: Endoscopy (2006)

The purpose of introducing optical electronics into video endoscopes is to improve the accuracy of diagnosis through image processing and digital technology. Narrow-band imaging (NBI), one of the most recent techniques, involves the use of interference filters to illuminate the target in narrowed red, green and blue (R/G/B) bands of the spectrum. This results in different images at distinct levels of the mucosa and increases the contrast between the epithelial surface and the subjacent vascular network. NBI can be combined with magnifying endoscopy with an optical zoom. The aim of this new technique is to characterize the surface of the distinct types of gastrointestinal epithelia - e. g., intestinal metaplasia in Barrett's esophagus. The technique may also make it possible to demonstrate disorganization of the vascular pattern in inflammatory disorders of the gastrointestinal mucosa and in superficial neoplastic lesions in the esophagus, stomach, and large bowel.
Early Closure of Myelomeningocele
Authors: V. C. Talwalker
Journal: BMJ (1967)

those making impulsive suicidal attempts.By the time a sufficient dose of the analgesic drug has been extracted from its wrappings some of the emotional heat might have been dissipated, allowing time for wiser counsels to prevail.It is so very much easier to open a bottle of tablets and swallow the lot in one impulsive gesture than it is to unwrap labori- ously each pill carefully embedded in tinfoil.We would like to suggest, therefore, as a contribution to lessening the self-poisoning problem, that the drug firms should be asked to consider whether it would be possible to market all sedative and hypnotic drugs in this individually wrapped fashion.We do not know how such packaging compares in cost with the more conventional methods of supply in cartons and bottles, but it is possible that any additional cost would be more than compensated for by the saving of lives and expensive hospital treatment.-We are, etc.,
D-penicillamine prevents the development of hepatitis in long-evans cinnamon rats with abnormal copper metabolism
Authors: Yuji Togashi, Yu Li, Jong-Hon Kang, Noritoshi Takeichi, Yasunori Fujioka, Kazuo Nagashima, Hiroshi Kobayashi
Journal: Hepatology (1992)

The Long-Evans Cinnamon rat is a mutant strain that contracts hereditary hepatitis and, eventually, spontaneous hepatocellular carcinoma. Because we found a corresponding gross copper accumulation in the liver of the rats, we examined whether the development of hepatitis in our rat system could be prevented by administration of D-penicillamine. D-Penicillamine is a copper-chelating agent and one of the drugs effective for human Wilson’s disease, in which abnormal copper metabolism is also observed. The results show that D-penicillamine treatment inhibited the elevation of serum transaminases, suppressed abnormal histological changes in the liver and completely prevented the onset of hepatitis in the Long-Evans Cinnamon rats. We further found that the copper concentration in the liver and serum copper and ceruloplasmin levels were decreased, whereas the urinary copper level was increased in the D-penicillamine—treated Long-Evans Cinnamon rats. These findings demonstrate that the pathogenesis of hereditary hepatitis in Long-Evans Cinnamon rats is due to abnormal copper accumulation in the liver. (Hepatology 1992;15:82-87).
Retroviral Gene Therapy in ApoE-Deficient Mice
Authors: Alyssa H. Hasty, MacRae F. Linton, Stephen J. Brandt, Vladimir R. Babaev, Linda A. Gleaves, Sergio Fazio
Journal: Circulation (1999)

Apolipoprotein E (apoE) has long been known to play an important role in the clearance of plasma lipoproteins. More recently, a direct role for apoE in delaying atherogenesis has been proposed. Macrophage production of apoE in the artery wall has been demonstrated to provide protection against atherosclerotic lesion development independently from its role in lipoprotein clearance. However, whether macrophage apoE can affect lesion growth at all stages of atherogenesis remains to be established.To evaluate the role of macrophage apoE in different stages of atherogenesis, as well as to establish a novel gene therapy approach to atherosclerotic vascular disease, we used an apoE-expressing retrovirus to transduce apoE-deficient (-/-) bone marrow for transplantation into apoE(-/-) recipient mice. Three weeks after bone marrow transplantation, apoE was expressed from arterial macrophages and was detectable in plasma associated with lipoproteins at 0.5% to 1% of normal levels but did not affect plasma cholesterol levels. We used 2 groups of recipient mice: younger mice with lesions consisting primarily of foam cells and older mice with more advanced lesions. When either the mouse or human apoE transgenes were expressed in mice from 5 to 13 weeks of age, there was a significant reduction in lesion area, whereas no effects were detected in mice that expressed apoE from 10 to 26 weeks of age.We demonstrate that arterial macrophage apoE secretion can delay atherogenesis if expressed during foam cell formation but is not beneficial during the later stages of atherogenesis. These data also provide evidence that apoE transgene expression from arterial macrophages may have therapeutic applications.
Nonspecific complement activation by streptococcal structures. II. Properdin-independent initiation of the alternate pathway.
Authors: J W Tauber, M J Polley, J B Zabriskie
Journal: The Journal of Experimental Medicine (1976)

Complement consumption by isolated membranes and walls from Group A streptococci and various other gram-positive microbes has been tested. These microbial structures were found to activate the alternate complement pathway. However, unlike endotoxin, inulin, or other plant polysaccharides, activation of complement by our material was found to bypass properdin. The activating factor(s) also differs from cobra venom in its/their requirement for factor D. Preliminary experiments suggest this factor isolated from membranes to be a protein and to have a mol wt greater than 40-60,000 daltons. Our studies have led us to speculate that the phylogenetic role of the alternate complement pathway may be the primordial nonspecific defense system which has retained certain fundamental aspects up to the present time.
ATYPICAL PHARYNGEAL DIPHTHERIA
Authors: S. C. LIND
Journal: JAMA (1913)

Notwithstanding the numerous articles on diphtheria in the various journals during the past two years, little or no mention is made of the atypical pharyngeal cases, A great deal has been published on typical clinical diphtheria, and on the carrier, his menace to the community and the methods of freeing such individuals from the bacillus. A rather complete examination of the literature of the past two years discloses no cases paralleling three that have come under my observation. We are apt to think of pharyngeal diphtheria as being a disease of sudden onset with general malaise, hyperemic throat in which a membrane rapidly forms, and finally terminates after a severe course. Once an exudate has appeared on the tonsils it is generally held that membrane formation progresses actively unless antitoxin is given. In Osler's "System of Modern Medicine" we find the following: Pharyngeal diphtheria commences with a feeling of chilliness,
Maltose- and maltotriose-modified, hyperbranched poly(ethylene imine)s (OM-PEIs): Physicochemical and biological properties of DNA and siRNA complexes
Authors: Sabrina Höbel, Andrea Loos, Dietmar Appelhans, Simona Schwarz, Jürgen Seidel, Brigitte Voit, Achim Aigner
Journal: Journal of Controlled Release (2010)
RBM20, a gene for hereditary cardiomyopathy, regulates titin splicing
Authors: Wei Guo, Sebastian Schafer, Marion L Greaser, Michael H Radke, Martin Liss, Thirupugal Govindarajan, Henrike Maatz, Herbert Schulz, Shijun Li, Amanda M Parrish, Vita Dauksaite, Padmanabhan Vakeel, Sabine Klaassen, Brenda Gerull, Ludwig Thierfelder, Vera Regitz-Zagrosek, Timothy A Hacker, Kurt W Saupe, G William Dec, Patrick T Ellinor, Calum A MacRae, Bastian Spallek, Robert Fischer, Andreas Perrot, Cemil Özcelik, Kathrin Saar, Norbert Hubner, Michael Gotthardt
Journal: Nature Medicine (2012)
Molecular Mechanisms of Anoxia/Reoxygenation-Induced Neutrophil Adherence to Cultured Endothelial Cells
Authors: Hiroshi Ichikawa, Sonia Flores, Peter R. Kvietys, Robert E. Wolf, Toshikazu Yoshikawa, D. Neil Granger, Tak Yee Aw
Journal: Circulation Research (1997)

The objectives of this study were to (1) determine the time course of neutrophil adhesion to monolayers of human umbilical vein endothelial cells (HUVECs) that were exposed to 60 minutes of anoxia followed by 30 to 600 minutes of reoxygenation and (2) define the mechanisms responsible for both the early (minutes) and late (hours) hyperadhesivity of postanoxic HUVECs to human neutrophils. The results clearly demonstrate that anoxia/reoxygenation (A/R) leads to a biphasic increase in neutrophil adhesion to HUVECs, with peak responses occurring at 30 minutes (phase 1) and 240 minutes (phase 2) after reoxygenation. Oxypurinol and catalase inhibited phase-1 adhesion, suggesting a role for xanthine oxidase and H2O2. In comparison, platelet activating factor (PAF) contributed to both phases of neutrophil adhesion. Anti-intercellular adhesion molecule-1 (ICAM-1) and anti-P-selectin antibodies (monoclonal antibodies [mAbs]) attenuated phase-1 neutrophil adhesion, consistent with roles for constitutively expressed ICAM-1 and enhanced surface expression of preformed P-selectin. Phase-2 neutrophil adhesion was attenuated by an anti-E-selectin mAb, indicating a dominant role of this adhesion molecule in the late phase response. Pretreatment with actinomycin D and cycloheximide or with competing ds-oligonucleotides containing the nuclear factor-kappa B or activator protein-1 cognate DNA sequences significantly attenuated phase-2 response, suggesting a role for de novo macromolecule synthesis. Surface expression of ICAM-1, P-selectin, and E-selectin on HUVECs correlated with the phase-1 and -2 neutrophil adhesion responses. Collectively, these findings indicate that A/R elicits a two-phase neutrophil-endothelial cell adhesion response that involves transcription-independent and transcription-dependent surface expression of different endothelial cell adhesion molecules.
GENETIC DETERMINANTS OF DRAMATIC IMPROVEMENT IN LEFT VENTRICULAR FUNCTION IN PATIENTS WITH HEART FAILURE
Authors: Maco V. Perez, Aleksandra Pavlovic, Matthew T. Wheeler, Frederick E. Dewey, Daniel Bernstein, Michael B. Fowler, Robert C. Robbins, Thomas Quertermous, Khin Chan, Devin Absher, Michael Ho, Elizabeth Cretti, Audrey Southwick, David Rosenthal, Richard M. Myers, Paul Heidenreich, Lisa Garrett, Daniel Sedehi, David Kao, Heidi Salisbury, Euan A. Ashley
Journal: Journal of the American College of Cardiology (2011)
Pre-existing renal disease promotes sepsis-induced acute kidney injury and worsens outcome
Authors: Kent Doi, Asada Leelahavanichkul, Xuzhen Hu, Karen L. Sidransky, Hua Zhou, Yan Qin, Christoph Eisner, Jürgen Schnermann, Peter S.T. Yuen, Robert A. Star
Journal: Kidney International (2008)