tbuckley/cabot-search huggingface dataset:
"Green-like" and "Red-like" RubisCO cbbL Genes in Rhodobacter azotoformans
Authors: Y. Uchino
Journal: Molecular Biology and Evolution (2003)

We found that Rhodobacter azotoformans IFO 16436T contains two different cbbL genes coding form I ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) large subunits. One gene is located within a “green-like” group of the RubisCO phylogenetic tree, and the other is located within a “red-like” group. This is the first report that one organism contains both green-like and red-like RubisCO genes. Moreover, by PCR using primers which amplify two green-like and red-like cbbL genes alternatively and dot blot hybridization, we demonstrated that Rhodobacter blasticus, Rhodobacter capsulatus, and Rhodobacter veldkampii possess only green-like cbbL genes, and Rhodobacter sphaeroides possesses only a red-like cbbL gene. In the cbbL phylogenic analysis, R. spaeroides and R. azotoformans 1 (red-like) formed a cluster within the red-like group, and R. capsulatus, R. azotoformans 2 (green-like), R. blasticus, and R. veldkampii formed a cluster within the green-like group. This suggests that red-like cbbL genes of Rhodobacter species were derived from one ancestor, and green-like cbbL genes were derived from another ancestor. On the other hand, molecular phylogeny of the bacteria indicates that R. veldkampii, which has only a green-like cbbL gene, is the earliest evolved Rhodobacter species and that R. azotoformans and R. sphaeroides, which have red-like cbbL genes, are the latest evolved. Consequently, the following hypothesis is proposed: the common ancestor of Rhodobacter had a green-like cbbL gene, the common ancestor of R. azotoformans and R. sphaeroides subsequently obtained a red-like cbbL gene by a horizontal gene transfer, and the ancestor of R. sphaeroides later lost the green-like cbbL gene.
Resveratrol inhibits proliferation, induces apoptosis, and overcomes chemoresistance through down-regulation of STAT3 and nuclear factor-κB–regulated antiapoptotic and cell survival gene products in human multiple myeloma cells
Authors: Anjana Bhardwaj, Gautam Sethi, Saroj Vadhan-Raj,, Carlos Bueso-Ramos, Yasunari Takada, Upasna Gaur, Asha S. Nair, Shishir Shishodia, Bharat B. Aggarwal
Journal: Blood (2006)
INFECTED FRACTURES: Two Cases Treated by Lane's Plates and Carrel Dressing
Authors: J. MacIntyre
Journal: BMJ (1918)

MARCH 9, I918 antiseptic towards the surface.Gauze soaked in Dakin's soluItioin was placed lightly on the tubes.The adjacent skin surface was spread with gauze soaked in sterilized vaseline; the (tressinig was completed by applyiug a large Carrel dressinig held in place by wooden clothes-line pegs.When the general cot (litioln of the patient made speed imperative, and tle local coni(litions ma(le complete operation (langerous, the inijured par4 was rapidly explored, an(d incisious miade to allow tlhe Carrel tubes to reach the depth of the woun(l.This plan was so satisfactory that a second alnd complete operation could be performed without danger of septic absorption.The surgeon, ssiisted by a sister, niurse, and orderly-no more thiani he bas for any other method-could carry out the after-dressings.'J'he same preparatioins and precautions were used as for an aseptic operation as far as sterilization of dressinigs, instru- ments, anid gloves is implie(l.The gloved hiaii(ns were never permiiitted to touch the dressinigs.Witth each of the team doing his or her allotted part, ten large dressings could be accomiilished in an hour.Sisters soo ibecame expert, and takinlg tle pict,ce of the surgeon, could carry out the dressings with success, tiie only difference being tthat the time occupied for eachl dlressinig was perlhaps a little longer.Bacteria coItnts wvere very efficienitly carried out by a V.A.D.nur-se trainetd in the worlk.The results were clhartedl.Varia- tions in the curve led to scrutiny of the techniiquie, anIld delay in apl)d inmprovement led one to suspect errors in techniique, such ais insufficient opening up of tracks, or, in comminutedl fracLures, necrosis of a doubtful fragmenit, or tubes badily placed.Results.Of 1,917 adlmissions, with 1,100 operations, there were 40 leaths.Many of these were admitted moribLund from trainis or hospital ships, and included 3 cases of tetanus, 2 of gas gangrene, 5 fracture(d femurs, and 2 unoperable neck wounds, 4 knee cases septicaemic on admission, 1 of secondary haemor- rhage which develope(d gangrene after necessary ligature of the femoral artery, 2 bluttock cases-one of thiese had lonigi-tL.hinalfracture of the lneck of the femur with infection of thie hip-joiint-anid mainyv severe head inijuries.The suiecess of the Carrel-Daliin metlhod was obvious from the first.Secondary hiaemorrhage practically con-I-
Alkaline phosphatase synthesis and properties of subcellular organelles during in vitro culture of jejunal biopsies from control subjects and patients with coeliac disease.
Authors: P E Jones, C L'Hirondel, T J Peters
Journal: Gut (1982)

Portions of jejunal biopsies from control subjects and from patients with coeliac disease were cultured for 24 hours using an in vitro organ culture technique. Alkaline phosphatase activity was measured in the tissue and medium before and after culture; enzyme activities were expressed per microgram tissue DNA. The increase in enzyme activity during the culture period was taken to represent net enzyme synthesis. Alkaline phosphatase synthesis by mucosa from patients with untreated gluten-sensitive coeliac disease and by mucosa from patients with non-responsive coeliac disease was significantly less than that by normal mucosa. Alkaline phosphatase synthesis by mucosa from patients with treated gluten-sensitive coeliac disease was greater than that by untreated coeliac mucosa but was less than normal mucosa. Sequential studies of alkaline phosphatase synthesis by jejunal mucosa from seven patients with coeliac disease, before and after successful treatment by gluten withdrawal, showed an increase in synthesis in all patients. Study, by analytical subcellular fractionation with sucrose density gradient centrifugation, of the properties of the organelles of cultured control tissue showed good preservation of their integrity. A striking finding, however, was the decrease in malate dehydrogenase with a corresponding marked increase in lactate dehydrogenase. This would be expected to be followed by a shift from aerobic to anaerobic metabolism. Analytical subcellular fractionation of cultured mucosa from patients with coeliac disease gave similar conclusions. There was, however, a marked improvement of the brush border abnormalities, characteristic of coeliac disease, during culture with increased enzyme activities and membrane equilibrium density in the sucrose gradients.
Obituary Listing
Authors: Laura L. Meyers
Journal: JAMA (1999)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 3472: Endothelial FAK is required for tumor angiogenesis
Authors: Bernardo Tavora, Kairbaan Hodivala-dilke
Journal: Cancer Research (2011)

Abstract Focal adhesion kinase (FAK) is a cytoplasmic tyrosine kinase that plays a fundamental role in integrin and growth factor mediated signalling and is an important player in cell migration and proliferation, processes vital for angiogenesis. However, the role of FAK in adult pathological angiogenesis is unknown. We have generated endothelial-specific tamoxifen-inducible FAK knockout mice by crossing FAK-floxed (FAKfl/fl) mice with the Pdgfb-iCreER mice. Tamoxifen-treatment of Pdgfb-iCreER;FAKfl/fl mice results in FAK deletion in adult endothelial cells (EC) without any adverse effects. Importantly however, endothelial FAK-deletion in adult mice inhibited tumour growth and reduced tumour angiogenesis. Furthermore, in in vivo angiogenic assays FAK-deletion impairs vascular endothelial growth factor (VEGF) induced neovascularisation. In addition, in vitro deletion of FAK in ECs resulted in reduced VEGF-stimulated AKT phosphorylation, and correlating reduced cellular proliferation and increased cell death. Furthermore, using a combination of genetic ablation and inhibition of FAK we are studying a new role of endothelial FAK in the regulation of the vascular barrier function with implications on the metastatic process. Our data suggest that FAK is required for adult pathological angiogenesis and suggests a possible role of endothelial FAK in the regulation of the vascular barrier function. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3472. doi:10.1158/1538-7445.AM2011-3472
SYSTEMATIC UNDERESTIMATION IN REPORTED MENTAL HOSPITAL READMISSION RATES
Authors: IRA FRIEDMAN, SVEN LUNDSTEDT, OTTO VON MERING, EDWARD N. HINKO
Journal: American Journal of Psychiatry (1964)

The present study represents an attempt to evaluate a systematic underestimation in reported mental hospital readmission rates. The case search of the medical records of 1137 admissions to the Cleveland Psychiatric Institute in 1957 resulted in discovery of 221 readmissions erroneously classified as first admissions. Corrections for this error elevated the reported crude readmission rate from 31.40% to 50.84%. Since the reported rate is roughly equivalent to nationally rates, and since the corrected rate is well above national averages (including those for Prolonged Care Hospitals and those for more recent years where higher rates are to be expected), the data suggest that a systematic reporting error may be widespread.
FEASIBILITY OF ROUTINE QUALITY OF LIFE ASSESSMENT IN AN AMBULATORY HEART FAILURE CLINIC AND IMPACT OF PATIENT PERCEPTIONS ON HEALTH STATUS
Authors: Sabrina Badloe, Parakash Pratibhu, Lynne W. Stevenson, Akshay Desai, Michelle A. Young, Joanne R. Weintraub, Michael M. Givertz, Eldrin F. Lewis
Journal: Journal of the American College of Cardiology (2010)
POTT'S ABSCESS OF THE SPINE OPENING INTO THE BRONCHI
Authors: J. M. Frawley
Journal: JAMA (1935)

Cases of Pott's disease of the spine with abscess opening into the bronchi are rare. The history of such a case is therefore reported. <h3>REPORT OF CASE</h3> A Mexican boy, aged 8 years, admitted to the General Hospital of Fresno County, June 5, 1933, had had fever and cough for a period of three months. During the last two months the cough had become productive of blood and pus. Three days before admission a high fever had developed. The boy complained of pain in the chest and coughed up some blood. When admitted he was emaciated and appeared ill. The temperature was 103.4 F., the pulse rate 124 and the respiration 26 per minute. There was dulness in the right upper part of the chest and râles were present throughout the entire right lung. The heart was not enlarged and there were no murmurs or thrills. Nothing further was found on examination. The child's father had died of pulmonary tuberculosis.
Alterations of the Dynamics in the Arterial Mesenteric Circulation Secondary to Atherosclerosi
Authors: JAMES E. OSKIN, JON M. ALEXANDER, CARLOS BEKERMAN, KENDALL L. CAPPECCI, GARY L. HERZOG
Journal: Clinical Nuclear Medicine (1991)

A 68-year-old woman with chronic ischemic bowel disease and intermittent gastrointestinal bleeding was re- Radiology, Michael Reese Hospital and Medical Center, ferred for a Tc-99m labeled red blood cell study to rule out a current Gl bleed. The examination demonstrated abnormal Tc-99m RBC accumulation in the left hemiabdomen without temporal or spatial change. Subsequent visceral angiography demonstrated delayed blood flow and persistence of the arterial phase, corresponding to the region of abnormal radionuclide activity. Both studies failed to reveal an active hemorrhage. The blood pooling or stasis of the Tc-99m RBC study may represent delayed blood flow or an increase in blood volume in a patient with severe atherosclerotic and ischemic bowel disease.
Few clues to diagnosis
Authors: Paul Emery, Jane Freeston
Journal: BMJ (2006)

Patient's parents with the support of S EllisWhen doctors asked us to participate we agreed without hesitation, knowing that it would bring new knowledge to the doctors and patients.When our daughter was first admitted to hospital, we thought she had an infection that could be simply and quickly cured.However, as time went on and all the tests returned negative, it became clear that there was something more sinister and it became increasingly frustrating that doctors could not come up with a definite diagnosis despite numerous investigations. 1 As she developed many rare complications associated with this illness, we naturally lost confidence in our attending doctors.Although the diagnosis of adult onset Still's disease was made, we still sought a second medical opinion because of our daughter's deterioration.Despite her complications, we were reassured and kept regularly informed of her development and progress.Now, we are working together with the doctors for further improvement.
Hepatitis A virus infection among homosexual men.
Authors: J Kani, R Nandwani, R J Gilson, A M Johnson, H C Maguire, R S Tedder
Journal: BMJ (1991)

<h3>Abstract</h3> <h3>Background</h3> Glycosylphosphatidylinositol (GPI) addition is one of the several post-translational modifications to proteins that increase their affinity for membranes. In eukaryotes, the GPI transamidase complex (GPI-T) catalyzes the attachment of pre-assembled GPI anchors to GPI-anchored proteins (GAPs) through a transamidation reaction. A mutation in <i>AtGPI8</i> (<i>gpi8-2</i>), the putative catalytic subunit of GPI-T in Arabidopsis, is transmitted normally through the female gametophyte (FG), indicating the FG tolerates loss of GPI transamidation. In contrast, <i>gpi8-2</i> almost completely abolishes male gametophyte (MG) function. Still, the unexpected finding that <i>gpi8-2</i> FGs function normally requires further investigation. Additionally, specific developmental defects in the MG caused by loss of GPI transamidation remain poorly characterized. <h3>Results</h3> Here we investigated the effect of loss of <i>AtPIG-S,</i> another GPI-T subunit, in both gametophytes. Like <i>gpi8-</i>2, we showed that a mutation in <i>AtPIG-S</i> (<i>pigs-1</i>) disrupted synergid localization of LORELEI (LRE), a putative GAP critical for pollen tube reception by the FG, yet is transmitted normally through the FG. Conversely, <i>pigs-1</i> severely impaired male gametophyte (MG) function during pollen tube emergence and growth in the pistil. A <i>pPIGS:PIGS-GFP</i> transgene complemented these MG defects and enabled generation of <i>pigs-1/pigs-1</i> seedlings, but seemingly failed to rescue the function of AtPIG-S in the sporophyte, as <i>pigs-1/pigs-1, pPIGS:PIGS-GFP</i> seedlings died soon after germination. <h3>Conclusions</h3> Characterization of <i>pigs-1</i> provided further evidence that the FG tolerates loss of GPI transamidation more than the MG and that the MG compared to the FG may be a better haploid system to study the role of GPI-anchoring. <i>pigs-1</i> pollen develops normally and thus represent a tool in which GPI anchor biosynthesis and transamidation of GAPs have been uncoupled, offering a potential way to study free GPI in plant development. While previously reported male fertility defects of GPI biosynthesis mutants could have been due either to loss of GPI or GAPs lacking the GPI anchor, our results clarified that the loss of mature GAPs underlie male fertility defects of GPI-deficient pollen grains, as <i>pigs-1</i> is defective only in the downstream transamidation step. Our study also provided further evidence that GPI transamidation is essential in seedling development.
Abstract C2: High-content imaging facilities modeling of cancer epithelial-mesenchymal transition signaling network
Authors: Xiaoping Zhu, Yin Zhen, Hong Zhao, Stephen Wong
Journal: Cancer Research (2013)

Abstract Background: The epithelial-mesenchymal transition (EMT) is critically involved in tumorigenesis and tumor progression except its fundamental role to development and tissue remodeling. Although the full spectrum of signaling agents that contribute to EMTs of carcinoma cells remains unclear, the interactions and coordination of individual known molecules or pathways that contribute to EMT phenotype as a network are not well studied. The EMT signaling network is complicate in nature, as such, a systems mathematical modeling is especially valuable to help understand and characterize genes that are mediated individually or in combination in the context of relevant signaling pathways, and such models would help to predict feedback loop in the network and provide candidate strategies to control the signaling transduction furthermore guide us make clinic research available after the further validation. Experimental design and methods: siRNA-mediated knockdown of individual nine EMT-related genes in mesenchymal like cancer cell line (HMLE-Ras-Snail and U87), and epithelial cell lines (HMLE-Ras-vector and MCF10A) were imaged in a time-lapse manner at single cell resolution. Multiple cell phenotype features were extracted by image informatics analysis. Thirteen proteins including the 9 siRNA targeted genes as well as 4 epithelial and mesenchymal marker proteins were profiled by Western blot. Bioinformatics modeling was performed to create a gene EMT network and describe the genes or pathway interactions which are dominant for EMT or MET phenotypes. Results: Each individual siRNA knockdown indicated by western blot, concomitantly incucyte captured clear cell morphology changes due to RNA silence: silencing TJP1, PXN, FLNA, CCNE1switched cells to mesenchymal-like, silencing Gli3, CTNNB1, PSEN1 induced cells more Epithelial-like. However not every maker protein showed as significant changes as images parameters (cell width/length ratio, protrusion ect) did. Therefore by using traditional biochemical and imaging two approaches, we will identify the confident genes for EMT regulation. Conclusion: Our results demonstrate that high-content imaging is a reliable and convenient tool to help us to distinguish crucial metastasis genes and related pathway from other ambiguous, redundant and broad genes network that is valuable for us to interpret the intricate EMT mechanism. Citation Format: Xiaoping Zhu, Yin Zhen, Hong Zhao, Stephen Wong. High-content imaging facilities modeling of cancer epithelial-mesenchymal transition signaling network. [abstract]. In: Proceedings of the AACR Special Conference on Tumor Invasion and Metastasis; Jan 20-23, 2013; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2013;73(3 Suppl):Abstract nr C2.
How dangerous is sledging?
Authors: J P Sloan, M Maheson, A F Dove
Journal: BMJ (1985)

the following 9 cm spinal needles with a Quincke point through the skin: 20 gauge, 22 gauge, 25 gauge (Becton Dickinson, Ireland), 23 gauge (Terumo Corporation, Japan), and 0-2 ml 1,, plain lignocaine5 (Astra Pharmaceuticals, Sweden) through a 25 gauge intravenous needle (Becton Dickinson, Ireland).The order of the needles (and thus the sites of insertion) was randomised.The volunteer was unaware of the needle size used or whether local anaesthetic had been infiltrated.After each needle or infiltration of lignocaine the volunteer completed the visual analogue scale.Results were analysed using the Wilcoxon signed rank test for paired observations.The volunteers' ages ranged from 26 to 54 years; two were women.The chart shows the pain scores.When the pain scores with the different needles were compared statistically significant differences existed between all the needles (p<0 01); an exception was the difference between the 22 gauge and 23 gauge needles, which was not significant.The finer needles caused less pain.There was also a significant difference (p< 0 01) between the pain scores of the 20 gauge, 22 gauge, and 23 gauge needles and the infiltration of lignocaine.There was no significant difference in pain scores between the 25 gauge needle and the infiltration of lignocaine.No learning effect was detected.The number of volunteers recording their lowest pain score was seen after the infiltration of local anaesthetic and the insertion of a 25 gauge needle.
Liver Harvesting Surgical Technique for the Treatment of Retro-Hepatic Caval Thrombosis Concomitant to Renal Cell Carcinoma: Perioperative and Long-Term Results in 15 Patients without Mortality
Authors: Michele Gallucci, Domenico Borzomati, Gerardo Flammia, Antonio Alcini, Giuseppe Albino, Marco Caricato, Alessandro Esposito, Bruno Vincenzi, Massimo Rossi, Roberto Coppola, Pasquale Berloco
Journal: European Urology (2003)
The Etiology of Human Breast Cancer: Endocrine, Genetic, Viral, Immunologic, and Other Considerations
Authors: William M. Cooper
Journal: JAMA (1975)

This small volume has an extensive bibliography and the text condenses many hours of reviewing the work of others as well as presenting the author's comments. The author seems to favor the immunological factors as the major ones in carcinogenesis in the breast, but thinks that genetic, viral, chemical, hormonal, and other factors may affect the malignant tumor development. He refers to the "immunological milieu" that must be suitable for the carcinogenic potential to be realized. Unfortunately, it appears that almost anything can affect this milieu. Thus, we are left with the same puzzle, namely, is there anything we can do to decrease the incidence of carcinoma of the breast? This book would be a useful reference because of the completeness of the bibliography, and I'm sure it would be read with interest by the clinician who does not have the opportunity of keeping up with the latest studies in
THE DIAGNOSIS AND TREATMENT OF CONVULSIONS IN CHILDREN.
Authors: H. LOWENBURG
Journal: JAMA (1907)

Eclamptic attacks in infants and young children are so common that perhaps some apology is necessary for presenting a paper on this subject. For the reason that one is privileged often to note cases wherein mistakes in diagnosis and subsequent treatment have been made, it may not be an untimely matter for discussion at this meeting. Convulsions<i>per se</i>do not constitute a morbid entity. The practitioner's disregard of this fact not infrequently leads to grave error in the management of an attack. It is insufficient to diagnose convulsions. By saying that a child has a convulsion one says no more than when he designates bleeding from the uterus or stomach respectively as menorrhagia or hematemesis. These are but scientific terms for symptoms. It becomes essential to diagnose the underlying cause of this symptom of nervous irritation in order to deal with it intelligently. Failure to recognize this elementary principle