tbuckley/cabot-search huggingface dataset:
Abstract 3896: The interaction between BRCA2 and DSS1 is crucial for homologous recombination through controlling protein localization
Authors: Akinori Egashira, Nicolas Siaud, Nicole Christ, Masaru Morita, Yoshihiro Kakeji, Maria Jasin, Yoshihiko Maehara
Journal: Cancer Research (2011)

Abstract The BRCA2 tumor suppressor plays an important role in the repair of DNA damage by homologous recombination (HR). Human BRCA2 is huge protein and is composed of several domains including DNA/DSS1 binding domain (DBD). DSS1 has shown to be important for HR in various organisms but the precise function of DSS1 through the interaction between BRCA2 is still unclear in mammalian cells. To clarify the role of DSS1-BRCA2 interaction in the repair of DNA damage, we put several mutations into BRCA2 peptides which could restore HR in Brca2 mutant hamster cells. The mutant BRCA2 peptides with mutation which reduced DSS1 binding impaired the ability of these peptides to promote HR when compared to wild type peptides. It is of great interest that the excessive DSS1 expression partially restored HR activity of BRCA2 mutants which weakly bind DSS1. Furthermore, excessive DSS1 expression facilitate the nuclear localization of wild type BRCA2 peptide. On the other hand, excessive DSS1 expression had no effect for neither HR activity nor the subcellular localization of mutant BRCA2 peptide that could not bind to DSS1. Knockdown of DSS1 by siRNA also showed reduced HR and nuclear localization of BRCA2 protein. In conclusion, one of the essential functions of DSS1 in HR through the interaction between BRCA2 might be a regulation of BRCA2 subcellular localization. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3896. doi:10.1158/1538-7445.AM2011-3896
An autosomal recessive exfoliative ichthyosis with linkage to chromosome 12q13
Authors: S.J. Hatsell, H. Stevens, A.P. Jackson, D.P. Kelsell, A. Zvulunov
Journal: British Journal of Dermatology (2003)

Journal Article An autosomal recessive exfoliative ichthyosis with linkage to chromosome 12q13 Get access S.J. Hatsell, S.J. Hatsell Centre for Cutaneous Research, Royal London School of Medicine, London Search for other works by this author on: Oxford Academic Google Scholar H. Stevens, H. Stevens Centre for Cutaneous Research, Royal London School of Medicine, London Search for other works by this author on: Oxford Academic Google Scholar A.P. Jackson, A.P. Jackson Molecular Medicine Unit, University of Leeds, Leeds, U.K Search for other works by this author on: Oxford Academic Google Scholar D.P. Kelsell, D.P. Kelsell Centre for Cutaneous Research, Royal London School of Medicine, London Search for other works by this author on: Oxford Academic Google Scholar A. Zvulunov A. Zvulunov Department of Paediatrics, Joseftal Hospital, Eilat and the Faculty of Health Sciences, Ben‐Gurion University, Beer Sheva, Israel Alex Zvulunov MD E‐mail: azvulun@bgumail.bgu.ac.il Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 149, Issue 1, 1 July 2003, Pages 174–180, https://doi.org/10.1046/j.1365-2133.2003.05386.x Published: 01 July 2003 Article history Accepted: 10 November 2002 Published: 01 July 2003
Effect of blood flow reversal in liver autotransplants upon the site of hepatocyte regeneration
Authors: Bernard Sigel, Liveo B. Baldia, Signe A. Brightman, Marvin R. Dunn, Raphael I. M. Price
Journal: Journal of Clinical Investigation (1968)

We studied the role of the direction of intrahepatic blood flow upon the location of hepatocyte formation in regenerating liver. Single liver lobes in the dog were autotransplanted to the region of the neck with the blood supply reestablished in a manner to perfuse the hepatic lobule from portal tract to central vein or, in a reverse direction, from central vein to portal tract. Partial resection of the nontransplanted liver was later performed to induce regeneration in the grafts by humoral means. Tritiated thymidine was administered, and radioautographs were prepared from excised graft and nontransplanted liver. In the "straight" blood flow grafts, as well as in all nontransplanted livers, labeled hepatocytes indicating DNA synthesis were found predominantly in the vicinity of the portal tracts. In the "reverse" blood flow grafts, labeled hepatocytes were more prevalent about the central veins. Thus, the localization of hepatocyte formation in the lobule during active liver regeneration cannot be attributed to an inherently greater capacity of periportal liver cells to divide but is probably related to their preferential exposure to blood constituent changes (humoral mechanisms). Hepatocyte regeneration in the presence of abnormal directional circulation might lead to lobular disorganization resulting in consequent biochemical aberrations despite the formation of new cells.
Infantile myofibromatosis: A series of 28 cases
Authors: Jacob Mashiah, Smail Hadj-Rabia, Anne Dompmartin, Annie Harroche, Etty Laloum-Grynberg, Michèle Wolter, Jean-Claude Amoric, Dominique Hamel-Teillac, Stéphane Guero, Sylvie Fraitag, Christine Bodemer
Journal: Journal of the American Academy of Dermatology (2014)
Fluoride for the Treatment of Osteoporosis
Authors: Jean Yves Reginster
Journal: Annals of Internal Medicine (1999)

Letters2 February 1999Fluoride for the Treatment of OsteoporosisGerson T. Lesser, MDGerson T. Lesser, MDJewish Home and Hospital; New York, NY 10025Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-130-3-199902020-00033 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:Reginster and colleagues (1) address the unresolved issue of fluoride supplementation and osteoporotic fractures. Unfortunately, their conclusion that “[l]ow-dose fluoride … given continuously with calcium for prolonged periods can decrease vertebral fracture rates …” is unsubstantiated and can be misleading.In discussing the ineffectiveness of fluoride in the 2-year Fluoride and Vertebral Osteoporosis (FAVOS) study (2), the authors endorse the highly reasonable assumption that positive bony changes “ … may require a prolonged period to ensure optimal biochemical resistance …” and “[t]herefore, a significant reduction in fracture rate might be expected only after 3 to 4 years ...References1. Reginster JY, Meurmans L, Zegels B, Rovati LC, Minne HW, Giacovelli G, et al . The effect of sodium monofluorophosphate plus calcium on vertebral fracture rate in postmenopausal women with moderate osteoporosis. A randomized, controlled trial. Ann Intern Med. 1998;129:1-8. LinkGoogle Scholar2. Meunier PJ, Sebert JL, Reginster JY, Briancon D, Appelboom P, Netter G, et al . Fluoride salts are no better at preventing new vertebral fractures than calcium-vitamin D in postmenopausal osteoporosis: the FAVOS study. Osteoporos Int. 1998;8:4-12. CrossrefMedlineGoogle Scholar Author, Article, and Disclosure InformationAffiliations: Jewish Home and Hospital; New York, NY 10025 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoThe Effect of Sodium Monofluorophosphate plus Calcium on Vertebral Fracture Rate in Postmenopausal Women with Moderate Osteoporosis Jean Yves Reginster , Laurence Meurmans , Brigitte Zegels , Lucio C. Rovati , Helmut W. Minne , Gianpaolo Giacovelli , Anne N. Taquet , Ivo Setnikar , Julien Collette , and Christiane Gosset Fluoride for the Treatment of Osteoporosis Valerie P. Weil Fluoride for the Treatment of Osteoporosis Jean Yves Reginster Metrics 2 February 1999Volume 130, Issue 3Page: 240KeywordsBone densityCalciumOsteoporosisSodium Issue Published: 2 February 1999 CopyrightCopyright © 1999 by American College of Physicians. All Rights Reserved.PDF DownloadLoading ...
Histamine release in skin monitored with the microdialysis technique does not correlate with the weal size induced by cow allergen
Authors: L. HORSMANHEIMO, I. T. HARVIMA, R. J. HARVIMA, J. YLÖNEN, A. NAUKKARINEN, M. HORSMANHEIMO
Journal: British Journal of Dermatology (1996)

Summary The purpose of this study was to monitor histamine release in immediate-type hypersensitivity reactions in the skin of 10 atopic patients, sensitive to cow, by using the microdialysis technique. Three healthy subjects, without any atopic features or background, served as the control group. The probe inserted into the forearm dermal skin was perfused with isotonic saline solution. Samples were collected at 15-min intervals. After the first allergen challenge of four prick tests close to the probe with cow allergen extract, the skin was similarly repricked again in five patients and three healthy subjects, and in five other patients, 25μl of 10μmol/l substance P (SP) was injected intracutaneously. The samples were analysed for histamine by radioenzyme assay. The patients were clinically evaluated for allergic symptoms, prick- and scratch-patch test reactivity and for serum cow-specific, and total, IgE levels. The baseline histamine concentration was 7·5±4·0 nmol/l (mean±standard deviation: SD; n=10). After the allergen challenge, the histamine concentration in the consecutive samples was 11·9±11·0 nmol/l, 91·1±127·3 nmol/l, 61·0±94·2 nmol/l and 33·7±53·7 nmol/l. The peak concentration was detected in the 15–30 min fraction, and it varied between 0 and 406 nmol/l regardless of the weal size. The second allergen challenge was unable to induce marked additional histamine release, but SP induced extensive histamine liberation in those patients who did not exhibit histamine release during the preceding prick tests. In three healthy subjects, the baseline histamine concentration was 6·2±3·9 nmol/l. After the allergen challenge, no additional histamine liberation could be measured. Surprisingly, the histamine release was not related to the size of the cow-induced weal nor was it related to any specific allergic symptoms or IgE levels. The results suggest that, in some patients, mast cell mediators other than histamine play a significant part in immediate-type allergic reactions of skin.
Voltage-dependent block of calcium channel current in the calf cardiac Purkinje fiber by dihydropyridine calcium channel antagonists.
Authors: M C Sanguinetti, R S Kass
Journal: Circulation Research (1984)

We have investigated the mechanisms of blockade of calcium channel current by the dihydropyridines, e.g. nisoldipine, nitrendipine, and nicardipine. Membrane current was recorded in isolated calf Purkinje fibers using a two-microelectrode voltage-clamp technique, and voltage protocols were designed to identify voltage- and use-dependent block by these compounds systematically. Our results show that calcium channel blockade by dihydropyridine derivatives is strongly modulated by membrane potential. Block is more pronounced when current is measured from depolarized holding potentials, but in contrast to verapamil, this voltage-dependent block occurs in the absence of repetitive depolarizations. Use-dependent block by dihydropyridines is observed at pulse frequencies greater than 1 Hz. Our results suggest that dihydropyridines bind preferentially to the inactivated state of the calcium channel, and that the development of use-dependent block is related to the ionization constants of the compounds. Furthermore, binding is approximately one thousand times stronger to inactivated channels than to resting channels. This state-dependent difference in binding affinities may account for the previously reported contrast between electrophysiological and binding data for these compounds.
The Physician and Marriage.
Authors: Francis Dean
Journal: JAMA (1902)

Denver, June 10, 1902. <h3>To the Editor:</h3> —At a recent meeting of a medical society the subject of how physicians may aid in securing proper marriage restrictions was discussed. One physician took the ground that the matter lay in the hands of the medical profession; that if the latter would make it a point to advise their constituents, that a great deal could be accomplished. A second thought a health certificate should be required as well as a license to marry. Another physician said his experience would not bear out the statement that the family physician could be of much service to the state by gratuitous advice. He gave it as his judgment that after two young people had determined to marry it was too late for the family physician to offer any suggestion; that whatever the physician could do had to be prior to that time. The experience of
Effectiveness of high‐dose intravenous immunoglobulin in a case of acquired von willebrand syndrome with chronic melena not responsive to desmopressin and factor VIII concentrate
Authors: Giancarlo Castaman, Alberto Tosetto, Francesco Rodeghiero
Journal: American Journal of Hematology (1992)

Abstract A patient with benign monoclonal lgG lambda paraproteinemia, acquired von Willebrand syndrome (AVWS), and chronic melena successfully responding to high‐dose intravenous immunoglobulin (lvlg) is reported. Coagulation parameters at admission were APTT (ratio) 1.68; VIII:C 11 IU/dL; vWF:Ag 7 IU/dL: Ricof &lt;3 IU/dl. RIPA was &gt;1.8 mg/ml, and bleeding time (BT) was prolonged (18 min). No evidence for an in vitro inhibitor against the VIII/vWF complex was observed. VIII/vWF measurements showed a short‐lived increase after both DDAVP and Hemate P, and BT was transiently normalized. After intravenous lg (1 g/kg for 2 days), VIII/vWF measurements, hemostatic parameters and multimeric pattern were completely corrected (VIII/C 106 IU/dl, vWF:Ag 168 IU/dl, RiC of 147 IU/dl, APTT ratio 0.89, BT 5′), with a return to pre‐infusion values after 15 days. Hemoccult test became negative and packed red cell transfusions, of which 130 units were administered during the last year, were no longer required. After 18 months the patient is on maintenance treatment with repeated courses of Ig, at 3 to 4‐week intervals based on VIII/vWF and BT monitoring. © 1992 Wiley‐Liss, Inc.
The use of the double-balloon enteroscope for endoscopic retrograde cholangiopancreatography and biliary stent placement after Roux-en-Y hepaticojejunostomy
Authors: T. Moreels, B. Roth, E. Vandervliet, P. Parizel, J. Dutré, P. Pelckmans
Journal: Endoscopy (2007)

Roux-en-Y biliodigestive reconstruction excludes the biliary tract from conventional endoscopic retrograde cholangiopancreatography (ERCP) [1] [2] [3]. However, the 200-cm-long double-balloon enteroscope (DBE) facilitates intubation of the afferent Roux-en-Y limb and maintains its position in front of the biliodigestive anastomosis. The 2.8-mm working channel allows for the introduction of conventional accessories, provided they have 230 cm length available. Choledochojejunostomy balloon dilation using DBE was first reported after living donor liver transplantation [4]. In one multicenter study, two patients successfully underwent DBE ERCP and biliary balloon dilation after Roux-en-Y reconstruction [5].
Nestle obeys Indian label laws
Authors: D. Briggs
Journal: BMJ (1995)

EDITOR,--It is disappointing that a professional journal like the BMJ should print stories in an obviously slanted way, and the inaccuracies of Claire Lockwood's news report1 need to be corrected.

The Indian court case is a direct result of a conflict between two laws which will have to be determined by the government or by the courts. One law requires the labels to contain the …
Abstract 2061: Implication of NQO1 knockdown on prostate carcinogenesis
Authors: Dinesh Thapa, Addanki P. Kumar, Rita Ghosh
Journal: Cancer Research (2012)

Abstract NAD(P)H-Quinone Oxidoreductase 1 (NQO1) is a key antioxidant enzyme that protects against quinone derived reactive intermediates and maintains a cellular pool of antioxidants. Recent studies from our laboratory and others have been found that the expression levels of NQO1 and several GST family genes are significantly suppressed in prostate tumors in preclinical mouse models. In this study, we investigated the role of NQO1 in prostate carcinogenesis. First, we characterized basal NQO1 expression in normal prostate cells (RWPE-1), initiated prostatic epithelium (BPH-1) and prostate cancer cells (LNCaP, C42B, PC-3 and DU145). Unlike in hormone-induced tumors in vivo, in-vitro study showed increased NQO1 expression in cancer cells compared to normal cells, and that finding was consistent with other cancer cell lines such as bladder, pancreatic and melanoma cells. Stable RNAi-mediated knockdown of NQO1 in prostate cancer cells increased cell proliferation and susceptibility to oxidant treatment. Furthermore, whole genome microarray revealed that NQO1 knockdown in LNCaP cells resulted in 1603 signature genes being deregulated. COL12A1, MCP-1/CCL2, IL-8, IL1R2 mRNA were among those that showed most highly significant increase (&amp;gt;6 fold; p&amp;lt;0.01)., whereas mRNA for nucleoplasmin 2, phosphotyrosine domain interacting protein PID1 and the melanoma tumor specific antigen gene family member MAGEC1 were highly significantly decreased (&amp;gt;12 fold; p&amp;lt;0.01). Investigations are under way to determine whether these genes are direct or indirect targets of NQO1. Thus, antioxidant NQO1 plays a significant but paradoxical role acting as a double-edged sword to regulate cellular processes. However, the mechanism by which the differential expression of these genes is regulated through NQO1 still remains to be elucidated. Blocking NQO1 may regulate ARE-related transcription factors, which in turn, possibly control antioxidant and pro-inflammatory pathways. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2061. doi:1538-7445.AM2012-2061
Gastrointestinal endoscopy in the young.
Authors: L N Forbat, R J Gribble, J H Baron
Journal: BMJ (1987)

Gastrointestinal endoscopy in the youngThe clinical value of standard procedures such as upper gastrointestinal endoscopy needs periodic scrutiny, preferably audit rather than debunking.Open access to endoscopy has been questioned and criteria for selection suggested.'We do not believe that the elderly should be excluded from
Worm infestation and the negative association with eczema (atopic/nonatopic) and allergic sensitization
Authors: T. Schäfer, T. Meyer, J. Ring, H.‐E. Wichmann, J. Heinrich
Journal: Allergy (2005)

Background: Worm infestations may play a role in preventing allergies. There is a lack of epidemiological information from Western countries on the association between worm infestation and eczema. Objective: To investigate the association between worm infestation and eczema in a proper temporal sequence and under consideration of allergic sensitization. Methods: Two surveys were performed in East German school children. Questionnaire data included the history of eczema and worm infestation and their time of onset. Specific IgE antibodies to five common aeroallergens were measured and used to define nonatopic and atopic eczema. Logistic regression analyses were performed to control for relevant confounders (age, sex, parental school education and history of allergies). In order to confirm the findings a corresponding conditional regression analysis was applied on cases and controls matched by age and sex. Results: A total of 4169 children participated (response 75 and 76%) who were, on average, 9.2 years old (47% girls). Overall 17.0% reported a prior worm infestation (Ascaris 44%, Oxyuris 33%) and 18.1% had a history of eczema. Eczema occurred significantly less frequent in children who had a worm infestation (prior to the onset of eczema) compared with children without such a history (8.1% vs 16.5%, OR adj : 0.45, 95% CI: 0.33–0.60). The finding was confirmed by the corresponding matched case–control analysis (OR adj : 0.57, 95% CI: 0.41–0.79). Atopic eczema was affected more by a prior worm infestation (OR adj : 0.31, 95% CI: 0.18–0.56) than the nonatopic eczema (OR adj : 0.58, 95% CI: 0.40–0.84). A total of 29.1% exhibited specific IgE antibodies to at least one aeroallergen. Sensitized children gave significantly less frequent a history of worm infestation (14.2% vs 18.3%, OR adj : 0.74, 95% CI: 0.60–0.92). Stratified analysis revealed that this effect most pronounced for a sensitization to house dust mite. Conclusions: A worm infestation is associated with a reduced frequency of subsequent eczema, especially the atopic type. Furthermore allergic sensitization, especially to house dust mite, and worm infestation are negatively associated. The data support the concept that a lack of immune‐stimulation by parasitic infections contributes to the development of allergies.
Hypnosis and Smoking
Authors: Sheldon B. Cohen
Journal: JAMA (1969)

Continuallyccumulating data<sup>1</sup>link smoking with a significant increase in morbidity and mortality in a variety of disease states. Many efforts have been and are being made to induce individuals to eliminate or reduce smoking. Hypnosis has been one of the psychological treatment methods espoused. Theoretically the hypersuggestibility inherent in hypnosis should make it an ideal vehicle for removal of an unwanted, undesirable, bad habit. Pragmatically, however, we must ask the following: How well does it work? What place does it have in the treatment of people who desire to stop smoking? For consideration two sets of data are presented: first, a survey of the literature; and second, findings from the use of hypnosis on ten patients, most with serious medical illness, who were specifically referred to a psychiatrist for assistance in stopping their smoking. <h3>Literature Survey</h3> While considering the scientific basis for anecdotal hypnotic cures, I studied the world medical
Therapeutic Arteriogenesis by Ultrasound-Mediated VEGF <sub>165</sub> Plasmid Gene Delivery to Chronically Ischemic Skeletal Muscle
Authors: Howard Leong-Poi, Michael A. Kuliszewski, Michael Lekas, Matthew Sibbald, Krystyna Teichert-Kuliszewska, Alexander L. Klibanov, Duncan J. Stewart, Jonathan R. Lindner
Journal: Circulation Research (2007)

Current methods of gene delivery for therapeutic angiogenesis are invasive, requiring either intraarterial or intramuscular administration. A noninvasive method of gene delivery has been developed using ultrasound-mediated destruction of intravenously administered DNA-bearing carrier microbubbles during their microcirculatory transit. Here we show that chronic ischemia could be markedly improved by ultrasound-mediated destruction of microbubbles bearing vascular endothelial growth factor-165 (VEGF 165 ) plasmid DNA. Using a model of severe chronic hindlimb ischemia in rats, we demonstrated that ultrasound mediated VEGF 165 /green fluorescent protein (GFP) plasmid delivery resulted in a significant improvement in microvascular blood flow by contrast-enhanced ultrasound, and an increased vessel density by fluorescent microangiography, with minimal changes in control groups. The improvement in tissue perfusion was attributed predominantly to increases in noncapillary blood volume or arteriogenesis, with perfusion peaking at 14 days after delivery, followed by a partial regression of neovascularization at 6 weeks. Transfection was localized predominantly to the vascular endothelium of arterioles in treated ischemic muscle. RT-PCR confirmed the presence of VEGF 165 /GFP mRNA within treated ischemic muscle, being highest at day 3 postdelivery, and subsequently decreasing, becoming almost undetectable by 6 weeks. We found a modulation of endogenous growth factor expression in VEGF-treated ischemic muscle, consistent with a biologic effect of ultrasound mediated gene delivery. The results of our study demonstrate the utility of ultrasonic destruction of plasmid-bearing microbubbles to induce therapeutic arteriogenesis in the setting of severe chronic ischemia.