tbuckley/cabot-search huggingface dataset:
Abstract 4928: 14-3-3ζ-mediated hypomethylation of c-myc oncogene by downregulation of Dnmts contributes to initiation and progression of breast cancer
Authors: Jia Xu, Dihua Yu
Journal: Cancer Research (2010)

Abstract The development of breast cancer is a multistep process and the molecular mechanisms underlying this process are still elusive. Previously, we discovered that 14-3-3ζ overexpression not only contribute to breast cancer progression but also play an important role in breast cancer initiation, including an early stage of breast disease-atypical ductal hyperplasia (ADH). However, the underlying mechanisms remain unclear. Recently, 14-3-3ζ has been shown to bind to Histone H3, Methylation Binding Proteins (MBPs) and HDAC family members, suggesting 14-3-3ζ may play a role in epigenetic regulation. As an initial effort to investigate whether 14-3-3ζ has a role in epigenetic regulation and whether epigenetic regulation by 14-3-3ζ plays a role in breast cancer initiation, we established 14-3-3ζ stable overexpressing lines (10A.14-3-3ζ) in non-tumorigenic MCF10A human mammary epithelial cells (HMEC) and analyzed molecular alterations by cDNA microarray in the 10A.14-3-3ζ cells versus the 10A.vec control cells in a 3D culture system. Interestingly, we found that c-myc, a well known oncogene which is overexpressed in many types of cancer, was upregulated at the mRNA level in the 10A.14-3-3ζ cells versus the 10A.vec cells. We further confirmed c-Myc upregulation at both mRNA level by RT-PCR and protein level by Western Blotting. Additionally, we performed Reverse Phase Protein Array (RPPA) analysis with a series of validated antibodies, and we found that Dnmt1, the key DNA methyltransferase in mammals, was downregulated in 10A.14-3-3ζ cells compared to 10A.vec cells. We have confirmed Dnmt1 downregulation by western blotting. Since c-myc oncogene are well known to be upregulated by hypomethylation as a consequence of Dnmt1 downregulation, these data suggest that 14-3-3ζ may contribute to breast cancer initiation and progression by upregulation of oncogene c-myc via promoter hypomethylation. We will continue this study to provide a better understanding on how 14-3-3ζ contributes to breast cancer initiation and progression via epigenetic deregulation (demethylation) of c-myc. This study will bring insight into how 14-3-3ζ overexpression elicits the phenotypic changes during the development of breast carcinomas through global deregulation of transcriptome as well as specific gene expressions. Our studies could provide a better understanding of the role of 14-3-3ζ in breast cancer initiation and progression. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4928.
Psychiatric Inpatients: Who, Where, and Future
Authors: LUCY D. OZARIN, CARL A. TAUBE
Journal: American Journal of Psychiatry (1974)

Recent statistics show that an increasing number of people with psychiatric illness are being treated in community settings rather than in hospitals. This shift in treatment locus requires a reexamination of program priorities, staff training and deployment, financial allocations, and plans for construction of facilities. A wide range of community-based therapeutic, rehabilitative, and supportive services appears necessary; the need for additional programs to treat patients with alcohol problems is particulary apparent.
TCT-527 What are the Predictors of Wound Healing in Patients with Critical Limb Ischemia with Tissue Loss following Successful Endovascular Therapy?
Authors: Norihiro Kobayashi, Toshiya Muramatsu, Reiko Tsukahara, Yoshiaki Ito, Hiroshi Ishimori, Keisuke Hirano, Masatsugu Nakano
Journal: Journal of the American College of Cardiology (2013)
Race, Ethnicity, and the Diagnosis of Breast Cancer
Authors: Bobby Daly, Olufunmilayo I. Olopade
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Subtotal splenectomy for the treatment of chronic lymphocytic leukemia
Authors: A Petroianu
Journal: Blood Cancer Journal (2015)

Although splenectomy is helpful in the management of selected patients with chronic lymphocytic leukemia (CLL), in most cases this procedure is accompanied by a greater morbidity and mortality, mainly due to sepsis. Thus, it may be proposed that a conservative procedure that reduces the spleen size may have an effect similar to that of total spleen ablation for the treatment of CLL. The present paper describes our experience with an 81-year-old patient submitted to subtotal splenectomy for treatment of CLL. Indications for surgery were uncontrolled leukemic activity and intense abdominal discomfort due to the huge spleen. The good results obtained with subtotal splenectomy in the present case indicate that this procedure may be a new alternative for the treatment of CLL when removal of the spleen is indicated.
UK doctors unlikely to be able to repay student loans, study finds
Authors: A. Rimmer
Journal: BMJ (2015)

UK doctors are unlikely to be able to fully repay their student loans over the course of their working lives, shows an analysis published in BMJ Open .1

The researchers, from the University of Birmingham, calculated that a medical student graduating in 2014 would have a debt of £40 000 (€55 000; $60 000) for tuition fees. If maintenance loans were factored in, this would add …
High Prevalence of Glucose Abnormalities in Patients With Hepatitis C Virus Infection
Authors: Albert Lecube, Cristina Hernández, Joan Genescà, Joan I. Esteban, Rosend Jardí, Rafael Simó
Journal: Diabetes Care (2004)

OBJECTIVE—The aim of this study was to compare the prevalence of both impaired fasting glucose (IFG) and diabetes between hepatitis C virus (HCV)-infected patients and patients with other liver diseases but anti-HCV−, taking into account the degree of liver damage. RESEARCH DESIGN AND METHODS—A total of 642 consecutive patients attending the outpatient liver unit of a university hospital (498 anti-HCV+ and 144 anti-HCV−) were prospectively recruited. Patients were classified as having chronic hepatitis (n = 472) or cirrhosis (n = 170) by means of the result of either a liver biopsy or by typical clinical features. A logistic regression model was used to determine independent associations of covariates (age, sex, BMI, HCV antibody status, and triglycerides) with the presence of glucose abnormalities. RESULTS—A threefold increase in the prevalence of glucose abnormalities was observed in HCV+ patients with chronic hepatitis in comparison with HCV− subjects (32 vs. 12%; P = 0.0003). In contrast, among patients with cirrhosis, although both diabetes and IFG were more prevalent in anti-HCV+ patients (40%) than in anti-HCV− patients (36%), the differences were not statistically significant. Finally, the logistic regression analysis showed that HCV infection was independently related to glucose abnormalities in those patients with chronic hepatitis (odds ratio 4.26 [95% CI 2.03–8.93]). In contrast, HCV was not an independent predictor of glucose abnormalities in cirrhotic patients. CONCLUSIONS—The high prevalence of IFG and diabetes found in HCV-infected patients observed in our study suggests that screening for glucose abnormalities should be indicated in these patients. In addition, we provide evidence that the genuine connection between HCV infection and diabetes is initiated at early stages of hepatic disease.
Bone marrow lipids in normal and anemic mice
Authors: Jane E. R. Potter, Eric G. Wright
Journal: American Journal of Hematology (1980)

Abstract The lipids in the bone marrow of six strains of normal mice, and mice with genetically determined anemias ( W / W v and S1 / S1 d ) and experimentally induced anemia were analyzed. Cholesterol and phospholipid accounted for 60–75% of total lipid; the remainder was triglyceride and neutral glyceryl ether lipids. In mice with phenylhydrazine (PHZ)‐induced hemolytic anemia there was a threefold increase in neutral glyceryl ether lipids immediately preceding a twofold increase in recognizable erythroid precursors. Comparisons were made between the femoral bone marrow and the cells maintained in the adherent populations of long‐term bone marrow cultures. In cultures of B6D2F 1 bone marrow (which support long‐term proliferation and differentiation of hemopoietic stem cells) the adherent layers contained the same lipids as freshly aspirated bone marrow. In W / W v and S1 / S1 d cultures (which duplicate, respectively, the stem cell and environmental defect found in vivo) the lipid content of the adherent layers differed from bone marrow, particularly in the absence of ether‐containing lipids. The results are consistent with the hypothesis that marrow lipids have an active (as opposed to passive) role in murine hemopoiesis and indicate that long‐term bone marrow culture may prove useful in further biochemical studies of the hemopoietic inductive microenvironment.
Effect of varying load magnitude on diaphragmatic glutathione metabolism during loaded breathing.
Authors: R Ciufo, D Nethery, A DiMarco, G Supinski
Journal: American Journal of Respiratory and Critical Care Medicine (1995)

Some studies have suggested that protective mechanisms downregulate diaphragm activity during loaded breathing so as to prevent respiratory-muscle fatigue. Other work has indicated, however, that loading can sometimes elicit significant diaphragmatic fatigue, and that the development of fatigue may be related to alterations in diaphragmatic glutathione concentrations. One potential explanation for these discrepant observations is that the mechanism of respiratory failure may vary as a function of load magnitude, and that some loads evoke little fatigue whereas others produce substantial fatigue and glutathione alterations. The purpose of this study was to examine this issue by determining the diaphragmatic fatigue and alterations in glutathione concentrations produced by a range of inspiratory resistive loads. Experiments were performed on decerebrate rats divided into a control, unloaded group and a group loaded with small, medium, and large inspiratory resistive loads that were applied until respiratory failure occurred. After respiratory arrest, the animals' diaphragms were excised, an in vitro determination was done of diaphragm contractility characteristics, and samples of muscle were assayed for GSH (reduced glutathione) and GSSG (oxidized glutathione). We found that in vitro diaphragm force generation was severely reduced for loaded breathing, and surprisingly, that the magnitude of the low-frequency fatigue present was similar in the three loaded groups. Reductions in diaphragmatic GSH levels and increases in GSSG levels were found in all three loaded groups. Reductions in diaphragmatic GSH levels and increases in GSSG levels were found in all three loaded groups, but again, the magnitude of these changes were similar.(ABSTRACT TRUNCATED AT 250 WORDS)
On Disorders of Articulate Speech
Authors: L. Stein
Journal: BMJ (1940)

The-aim of this paper is to show why some people, born and brought up in the same environment as ourselves, may yet develop a special form of speech, such as lisping or one of the allied disorders. For our purpose it is, I think, advisable to look first at the way in which Nature has developed articulation. The word  articulation  is of Latin origin ; articulus is derived from the stem ar?  to join. When used to describe sounds the adjective  articulate  means  divided into distinctf and significant parts  :  speaking intelligibly. The verb  to articu late  means the action that leads to the state expressed by the former. The word  significant  implies a conscious intention to make signs, and thus also a relation whose parts are: (i) the individual who makes the signs, and (ii) the individuals to whom they are directed.
Clinical Evaluation of Benzthiazide, an Oral Diuretic
Authors: C. W. H. Havard, P. H. N. Wood
Journal: BMJ (1960)

The synthesis of chlorothiazide has stimulated the search for other substitution derivatives of the benzothiadiazine nucleus in the hope that these newer compounds might be less prone to induce potassium depletion.The newest compound belonging to this group is benzthiazide (3 -benzylthiomethyl -6 -chloro -7 -sulphamyl -1,2,4 - benzothiadiazine-1,1-dioxide) (Fig. 1).The mode of action of chlorothiazide has been studied extensively (Bayliss et al., 1958; Matheson and Morgan, 1958; Watson et al., 1958), and a direct comparison with this drug provides the most satisfactory method of assessing the value of a new compound of this type.
Immunohemolytic Anemia During Therapy With Methyldopa
Authors: Ferid Murad
Journal: JAMA (1968)

Immunohemolytic anemia secondary to methyldopa therapy occurred in an adult. Folate deficiency was also present because of rapid red blood cell turnover induced by the hemolysis. The globulin responsible for the positive direct and indirect Coomb's tests was of the warmacting IgG variety, with specificity for the Rh locus. The degree of hemolysis decreased and the hematocrit reading increased when methyldopa therapy was discontinued and prednisone and folate sodium were given. The Coomb's tests remained positive nine months after treatment was begun.
A Red Blood Cell Agglutination <scp>d</scp>-Dimer Test to Exclude Deep Venous Thrombosis in Pregnancy
Authors: Wee-Shian Chan, Sanjeev Chunilal, Agnes Lee, Mark Crowther, Marc Rodger, Jeffrey S. Ginsberg
Journal: Annals of Internal Medicine (2007)

Background: d-Dimer testing is often used with compression ultrasonography for the diagnosis of deep venous thrombosis (DVT) in nonpregnant patients. The d-dimer test is highly sensitive, and a negative result can obviate the need for further testing for DVT. This test has not been studied for DVT diagnosis in pregnancy because its specificity was deemed too poor. Objective: To determine the sensitivity and specificity and assess the utility of the SimpliRED assay (Agen Biomedical, Brisbane, Australia) for the diagnosis of DVT in pregnant women. Design: Prospective cohort study. Setting: 5 tertiary university-affiliated Canadian hospitals providing care to obstetric patients. Patients: 149 consecutive pregnant women with suspected DVT who presented to 1 of the participating centers over 5 years. Intervention: Participating women were tested with compression leg ultrasonography (single or serially on days 0, 3, and 7) and received 3 months' clinical follow-up for the presence or absence of DVT. Whole blood was tested with the SimpliRED assay at initial presentation, and results were correlated with ultrasonography and clinical findings for the presence or absence of DVT. Measurements: The sensitivity, specificity, and negative predictive value of the SimpliRED assay were calculated, along with the prevalence of false-positive SimpliRED assay results (with 95% CIs). Results: The prevalence of DVT in the cohort was 8.7% (95% CI, 5.2% to 14.4%). The sensitivity of the SimpliRED assay was 100% (CI, 77% to 100% [13 of 13 patients]), the specificity was 60% (CI, 52% to 68% [81 of 135]), and the negative predictive value was 100% (CI, 95% to 100% [81 of 81]). The SimpliRED assay was positive in 0% (CI, 0% to 60%), 24% (CI, 14% to 37%), and 51% (CI, 40% to 61%) of women in the first, second, and third trimesters, respectively, among pregnant patients in whom DVT was not diagnosed. Limitations: The prevalence of DVT in the cohort was low, resulting in wide CIs. The clinician's previous impression was used to determine pretest probability in the absence of a validated clinical prediction rule for pregnant women. Conclusion: The SimpliRED assay may be useful in pregnancy because a normal result excludes DVT and occurs frequently enough to be clinically helpful.
Suicide rate of women doctors in US is twice that of other working women
Authors: Roger Dobson
Journal: BMJ (2007)

The proportion of female doctors who kill themselves is twice that of other working women, finds a study of self inflicted deaths in 26 US states over nine years.

No overall difference was shown between male doctors and men in other jobs in the proportion who committed suicide—although among men over the age of 45 years the proportion was higher in doctors, the study …
Smoking and Acute Respiratory Illness
Authors: Mark D. Aronson
Journal: JAMA (1983)

To the Editor.—   important reference was not included in our article entitled An Association Between Cigarette Smoking and Acute Respiratory Tract Illness in Young Adults (1982;248:181). In 1966, Haynes and co-workers1studied a group of male adolescents in a boys' preparatory school. Their article differed from ours in that they did not study a control group (in our study we used aged-matched women with acute genitourinary illnesses) and in the high smoking rate of 48% reported. (This figure far exceeds the national rate of approximately 10% to 15% smokers for an adolescent age group.) Their findings are consistent with ours and with the notion that cigarette smoking is a substantial risk factor for both the development and increased severity of acute respiratory tract illnesses in young smokers before obvious chronic lung disease has developed.
THE DEKMATOPHYTOSES OF GREAT BRITAIN. REPORT OF A THREE YEARS' SURVEY.
Authors: JACQUELINE WALKER
Journal: British Journal of Dermatology (1950)

Journal Article THE DEKMATOPHYTOSES OF GREAT BRITAIN. REPORT OF A THREE YEARS' SURVEY. Get access JACQUELINE WALKER JACQUELINE WALKER Mycological Reference Laboratory, Public Health Laboratory Service, London School of Hygiene and Tropical Medicine Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology and Syphilis, Volume 62, Issue 6, 1 June 1950, Pages 239–251, https://doi.org/10.1111/j.1365-2133.1950.tb14379.x Published: 01 June 1950
Quality in Cancer Diagnosis
Authors: S. S. Raab, D. M. Grzybicki
Journal: CA A Cancer Journal for Clinicians (2010)

Improving the quality of oncologic pathology diagnosis is immensely important as the overwhelming majority of the approximately 1.6 million patients who will be diagnosed with cancer in 2010 have their diagnoses established through the pathologic interpretation of a tissue sample. Millions more patients have tissue samples obtained to rule out cancer and do not have cancer. The majority of studies on the quality of oncologic pathology diagnoses have focused on patient safety and have documented a variety of causes of error that occur in the clinical and pathology laboratory testing phases of diagnostic testing. The reported frequency of a diagnostic error made by oncologic pathology depends on several factors, such as definitions and detection methods, and ranges from 1% to 15%. The large majority of diagnostic errors do not result in severe harm, although mild to moderate harm in the form of additional testing or diagnostic delays occurs in up to 50% of errors. Clinical practitioners play an essential role in error reduction through several avenues such as effective test ordering, providing accurate and pertinent clinical information, procuring high-quality specimens, providing timely follow-up on test results, effectively communicating on potentially discrepant diagnoses, and advocating second opinions on the pathology diagnosis in specific situations.
FGF signaling facilitates postinjury recovery of mouse hematopoietic system
Authors: Meng Zhao, Jason T. Ross, Tomer Itkin, John M. Perry, Aparna Venkatraman, Jeffrey S. Haug, Mark J. Hembree, Chu-Xia Deng, Tsvee Lapidot, Xi C. He, Linheng Li
Journal: Blood (2012)
MENSTRUATION AND THE SAFE PERIOD-Reply
Authors: Emil Novak
Journal: JAMA (1934)

To the Editor.—  Your correspondent is perfectly right in his first comment, but not in his second. While the context makes my real meaning clear enough, I regret exceedingly that the slip in words escaped me in reading the proof of my paper. The word nondangerous should, of course, have been dangerous. As for the intricacies of which the correspondent speaks, I believe that these apply only to the case of women with irregular cycles, in whom, as I stated in the paper, there is a greater element of fallibility in the determination of the   than there is in women with regular four-weekly cycles. In the latter, since menstruation recurs every twenty-ninth day, there is no difference between the Knaus plan of counting forward ten to seventeen days from the first day of the preceding period to derive the safe span, and the Ogino method of counting back
Alcohol-induced sensitivity and tolerance
Authors: S. Vaisrub
Journal: JAMA (1972)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal