tbuckley/cabot-search huggingface dataset:
364. The Effect of Overexpression of Vascular Endothelial Growth Factor in Mouse Embryonic Stem Cells on Angiogenesis
Authors: Ying Ka Lau, Jennifer L. West
Journal: Molecular Therapy (2006)

Angiogenesis is important in tumor biology, ischemic diseases, wound healing and many other areas. In our study, we studied the effect of overexpression of vascular endothelial growth factor (VEGF) in mouse embryonic stem cells (10T1/2 cells)through retroviral transfection. Blood vessels are made of 2 types of cells namely perictyes/smooth muscle cells and endothelial cells. 10T1/2 cells are considered to be the progenitor cells of pericytes/smooth muscle cells. 10T1/2 cells do not express VEGF when they are cultured alone and our study showed that even after co-culturing them with endothelial cells (ECs) for more than 5 days did not induce VEGF production. We showed that 10T1/2 cells were being able to be transfected by this method which resulted in overexpression of the VEGF transgene as well as the GFP transgene (our control). We embedded the 10T1/2 cells in collagen matrices and co-cultured these with ECs in an insert system with the collagen matrices on top of the insert. Our results showed that proliferation of ECs was significantly increased as compared with the control. In another co- culture system, we seeded 10T1/2 cells on the bottom of the wells and ECs on top of inserts to study the effect of overexpression of VEGF in 10T1/2 cells on migration of ECs. Migration of ECs was determined by the number of cells which had migrated to the other side of the inserts through the pores of the inserts. Migration of ECs was signicantly increased when they are co-cultlured with VEGF- producing 10T1/2 cells. We then studied the change in morphology of ECs in 3D collagen matrices when co-cultured with 10T1/2 cells. ECs formed organized tubular patterns in collagen matrices when co-cultured with VEGF-producing 10T1/2 cells as compared with control which showed no such patterns. We conclude that overexpression of VEGF in 10T1/2 cells could lead to increase in proliferation and migration as well as morphological changes in ECs. This might find applications such as angiogenesis for curing ischemic diseases, increased endothelialization in tissue engineered vascular grafts and vascularization of tissue engineering scaffolds.
Low Immunohistochemical Expression of MGMT in ACTH Secreting Pituitary Tumors of Patients with Nelson Syndrome
Authors: Fateme Salehi, Bernd W. Scheithauer, Veronica J. Moyes, William M. Drake, Luis V. Syro, Branavan Manoranjan, Soniya Sharma, Eva Horvath, Kalman Kovacs
Journal: Endocrine Pathology (2010)
Patient Rejection of Lithium Carbonate Prophylaxis
Authors: Phillip Polatin
Journal: JAMA (1971)

In the milder forms of manic-depressive disorders, many patients refuse to continue with lithium carbonate as a prophylactic agent for the following reasons: (1) Creative patients feel that the lithium carbonate acts as a "brake," preventing productivity and interfering with the earning of a livelihood. (2) The hypomanic phase is so enjoyable that rather than eliminate or limit it, they prefer to take their chances on the possibility that a depression will not recur. (3) Denial of the chronicity of the illness lies behind denial of the need to ingest lithium carbonate indefinitely.
Sleep Onset REM Periods during Multiple Sleep Latency Tests in Patients Evaluated for Sleep Apnea
Authors: RONALD D. CHERVIN, MICHAEL S. ALDRICH
Journal: American Journal of Respiratory and Critical Care Medicine (2000)

Although 2 or more sleep onset rapid eye movement (REM) periods (2omSOREMPs) on a Multiple Sleep Latency Test (MSLT) raise the possibility of narcolepsy, patients with obstructive sleep apnea (OSA) also can have 2omSOREMPs, which may then cause diagnostic uncertainty. To explore what features among OSA patients predict 2omSOREMPs on an MSLT that follows nocturnal polysomnography, we reviewed data from 1,145 consecutively studied patients suspected or confirmed to have OSA rather than narcolepsy. Overall, 4.7% of the subjects had 2omSOREMPs. Variables that were independently predictive of 2omSOREMPs in logistic regression models included male gender (OR = 4.4, 95% CI = 1.9 to 12.7), a 5-min decrease in the MSLT-derived mean sleep latency (OR = 1.9, 95% CI = 1.3 to 2.8), a 90-min decrease in nocturnal latency to REM sleep (OR = 1.6, 95% CI = 1.1 to 2.5), and a 15-unit decrease in minimal recorded oxygen saturation (OR = 1.6, 95% CI = 1.3 to 2.0). We conclude that among patients suspected or confirmed to have OSA, one or more of these four variables-male sex, sleepiness, nocturnal REM sleep latency, and extent of oxygen desaturation-could reflect neurophysiological mechanisms responsible for 2omSOREMPs. Consideration of these variables, and especially gender, may be useful in clinical practice when 2omSOREMPs are found unexpectedly.
Big Particle Biopsy
Authors: R. Ottenjann, G. Lux, M. Henke, M. Strauch
Journal: Endoscopy (1973)

Specimens obtained by conventional forceps biopsy technique are not always adequately large and deeply excised to get an exact histological diagnosis. For this reason big particle biopsy is recommended especially in the differential diagnosis of giant folds including hyperplasia of gastric mucosa and exophytic gastrointestinal tumours. Big particle biopsy was taken in a total of 11 patients. The diameter of the particles was 8 to 20 mm, excision depth was up to 4 mm. In all cases the muscularis mucosae was included, in several cases layers of the submucosa. Additional information was obtained in 7 of 9 cases where comparison with the forceps biopsy was possible. No complications were abserved.
Genetics Manual: Current Theory, Concepts, Terms
Authors: Michael R. Cummings
Journal: Annals of Internal Medicine (1999)

Medical Writings19 January 1999Genetics Manual: Current Theory, Concepts, TermsMichael R. Cummings, PhDMichael R. Cummings, PhDUniversity of Illinois at Chicago, Chicago, Illinois.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-130-2-199901190-00032 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Genetics Manual: Current Theory, Concepts, TermsRedei GP. 1142 pages. River Edge, NJ: World Scientific; 1998. $78.00. ISBN 9810227809. Order phone 800-227-7562.Field of medicine: Genetics.Format: Hardcover book.Audience: Medical and graduate students; scientists; physicians; and nonspecialists seeking clear, concise explanations of genetic concepts, theories, and terms.Purpose: To serve as a comprehensive reference source covering all aspects of modern genetics.Content: This book includes approximately 18 000 entries with concise descriptions of genetic concepts, techniques, and theories. Each entry is presented in a few paragraphs and is cross-referenced to related entries with occasional references to key papers in ... Author, Article, and Disclosure InformationAffiliations: University of Illinois at Chicago, Chicago, Illinois. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 19 January 1999Volume 130, Issue 2Page: 168KeywordsGenetic disordersGenetic techniquesGeneticsGraduate medical educationHuman geneticsPhenotypesRNARiversScientistsTouch ePublished: 15 August 2000 Issue Published: 19 January 1999 Copyright & PermissionsCopyright © 1999 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Clinical disorders of iron metabolism.
Authors: William R. Best
Journal: JAMA (1963)

<b>Iron metabolism</b>. By Thomas H. Bothwell and Clement A. Finch. 440 p. $15. Little, Brown &amp; Co., 34 Beacon St, Boston 6, Mass., 1962 Iron deficiency, the world's most common cause of anemia, has been found in the United States in more than one fourth of young children and in more than one tenth of young and middle-aged women. It always represents a need for iron in excess of the actual amount absorbed. Body needs are greater than average during periods of growth, during pregnancy, and when menstrual losses are excessive. A diet poor in iron may fail to meet such increased needs, but is rarely, if ever, the sole cause of iron deficiency. Occult gastrointestinal blood loss, from malignancy or other lesion, is the most common cause of iron deficiency in patients who demonstrate none of the physiologic reasons for increased iron need. Fully-developed iron deficiency anemia is readily
An inconvenient truth: urgent care is not primary care
Authors: D. Loxterkamp
Journal: BMJ (2015)

Primary care is best at placing illness in context



In February 2014 the biggest seller of prescription drugs in the United States, CVS Caremark, announced that it would end the sale of tobacco and tobacco products. The decision would cost the company an estimated $2bn (£1.3bn; €1.8bn) a year, but that represented only a fraction of the $127bn in total sales.1 The company then changed its name to CVS Health, signaling its desire to become a healthcare company, not just a chain of retail pharmacies. In fact, the transformation of CVS was foreshadowed years earlier when it opened its first in-store medical clinic.

Today CVS is the largest operator of walk-in health centers in the US, with its MinuteClinics in more than 900 stores and 31 states. They are staffed by nurse practitioners and physician assistants who treat minor illnesses and accidents and carry out health screening and preventive examinations, tests, and vaccinations at roughly half the cost of a regular doctor’s visit. Andrew Sussman, president of MinuteClinics and a doctor, sees walk-in centers as filling a void. Half the patients who …
DHMEQ, a new NF-κB inhibitor, induces apoptosis and enhances fludarabine effects on chronic lymphocytic leukemia cells
Authors: R Horie, M Watanabe, T Okamura, M Taira, M Shoda, T Motoji, A Utsunomiya, T Watanabe, M Higashihara, K Umezawa
Journal: Leukemia (2006)
Excess 18-Hydroxydeoxycorticosterone: Most or Some?
Authors: JAMES C. MELBY
Journal: Annals of Internal Medicine (1972)

Letters, Comments, and Corrections1 February 1972Excess 18-Hydroxydeoxycorticosterone: Most or Some?JAMES C. MELBY, M.D.JAMES C. MELBY, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-76-2-334_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: Dr. M. H. Maxwell states in the UCLA Conference, "Hypertension—Primary and Secondary" (Ann Intern Med75:761-776, 1971), that "about 20% of all patients with so-called essential hypertension have suppressed plasma renin activity. This has been unexplained, but it is exciting that investigators are finding that most of these patients have an excess of 18-hydroxydeoxycorticosterone." Although Dr. Maxwell does not refer specifically to the published evidence for this assertion, my colleagues and I have, for the past few years, been measuring metabolites of 18-OH-DOC in the urine of patients with low renin hypertension and find that not more... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Section of Endocrinology and Metabolism University Hospital Boston University Medical Center Boston, Mass. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 February 1972Volume 76, Issue 2Page: 334-334KeywordsHypertensionMetabolitesPlasma renin activityUrine Issue Published: 1 February 1972 PDF DownloadLoading ...
Normal Disposition of Oxazepam in Acute Viral Hepatitis and Cirrhosis
Authors: HARRISON J. SHULL
Journal: Annals of Internal Medicine (1976)

Oxazepam (Serax®) is a tranquilizer-sedative of the benzodiazepine group that is predominantly metabolized to a pharmacologically inactive glucuronide and subsequently excreted by way of the kidneys. We administered this drug as a single oral dose to seven patients with acute viral hepatitis, to six with cirrhosis, and to age-matched control subjects. Elimination half-life (T½) and the apparent oral plasma clearance for the drug in patients with hepatitis and cirrhosis were comparable to values obtained in age-matched controls (P > 0.05). In addition, the apparent volume of distribution of oxazepam, its plasma binding, blood/plasma ratio, and the rate of urinary excretion of oxazepam, predominantly as the glucuronide, were comparable (P > 0.05) in the two groups of patients with liver disease and their respective controls. Unlike many other sedatives, oxazepam is eliminated normally in patients with parenchymal liver disease and therefore, on pharmacokinetic grounds, seems to be an excellent sedative for use in such persons.
Russell body gastritis
Authors: Claudine Habib, David L. Gang, Rony Ghaoui, Liron Pantanowitz
Journal: American Journal of Hematology (2010)

Russell body gastritis, first described in 1998 [1], is a reactive gastric mucosal infiltration of plasma cells filled with cytoplasmic Russell bodies. Russell bodies are immunoglobulins present within dilated rough endoplasmic reticulum cisternae that have accumulated secondary to abnormal secretion [2]. We present a case of Russell body gastritis occurring in a 75-year-old man with a history of alcohol use, renal failure, dyslipidemia, and prior rhabdomyolysis. He was noncompliant with reflux medications and reported intermittent coffee-ground emesis. Esophagogastroduodenoscopy with biopsy revealed esophagitis and nodular chronic active gastritis. The gastric antral mucosa exhibited regenerative changes and a dense chronic inflammatory infiltrate (Image 1) composed of numerous Russell body filled plasma cells, including Mott cells (Image 2). No lymphoepithelial lesions were identified. A CD138 immunostain (Image 3) confirmed that these were plasma cells and in situ hybridization studies for kappa and lambda light chains demonstrated their polyclonal distribution. Gastric biopsies were negative for Helicobacter pylori by immunohistochemistry. Regenerative gastric mucosa showing numerous plasma cells filled with bright eosinophilic Russell bodies (H&E stain; original magnification ×200). Plasma cells shown at higher magnification are filled with cytoplasmic Russell bodies (H&E stain; original magnification ×600). Plasma cells show strong CD138 immunoreactivity (immunohistochemical stain; original magnification ×600). To date, there have been 11 other published case reports of Russell body gastritis [1-10]. The average patient age is 57 years (range, 29–80 years), including seven males and five female patients. These patients presented primarily with gastric related symptoms including nausea, dyspepsia, and epigastric pain. Mucosal swelling and erythema appear to be relatively common endoscopic findings, and only a few cases exhibited erosion, ulceration, or a raised lesion endoscopically. The exact etiology of Russell body gastritis is unclear. However, nine (82%) of these reported cases were H.pylori positive, two individuals were positive for human immunodeficiency virus (HIV), and one also had Candida esophagitis. Another two patients had Epstein Barr virus (EBV)-associated gastric carcinoma. Except for one individual with a concomitant monoclonal gammopathy of undetermined significance (MGUS), none of these patients have manifested a plasma cell disorder. Russell body gastritis is a diagnosis based on microscopic findings. The differential diagnosis on biopsy includes signet ring cell carcinoma, plasmacytoma, B-cell lymphoma with plasmacytic differentiation, and granular cell tumor. As highlighted in this, ancillary studies can be useful to demonstrate that these cells are polytypic plasma cells. Associated gastric carcinoma and infection (H.pylori, HIV, EBV, Candida) should also be excluded.
Modulation of the IgE response to Alternaria after DNA vaccination with the major Alternaria allergens (r Alt a1 and r Alt a2) in a brown Norway rat model
Authors: M.M. Montalbano, H. Sanchez, E.L. Keyes, L.A. Rosenthal, R.L. Sorkness, R.K. Bush
Journal: Journal of Allergy and Clinical Immunology (2003)
The Principles of Ultrasonography in Obstetrics and Gynecology
Authors: Donald L. King
Journal: JAMA (1991)

The editors have compiled an outstanding resource for the professional in the field of ultrasound concerned with obstetrics and gynecology. Sixty-four authors have contributed 44 chapters covering the breadth and depth of this important and dynamic field. New developments such as transvaginal ultrasonography, sonographically directed interventions, and Doppler studies of pelvic circulation are well covered. This edition represents an almost complete revision of the previous edition, with new chapters concerning fetal blood sampling, fetal interventional procedures, and early detection of ovarian carcinoma. Other chapters go beyond the traditional to include sonography of the breast and a discussion of legal concerns related to sonography, particularly with respect to obstetric practice. This is an authoritative reference work covering the field from the basics of ultrasound physics and bioeffects to the subtleties of fetal anomalies. Each chapter is carefully referenced, providing the student with access to primary sources. This book is highly recommended
Operant Conditioning in a Vietnamese Mental Hospital
Authors: LLOYD H. COTTER
Journal: American Journal of Psychiatry (1967)

Operant conditioning was applied in the special circumstances of a Vietnamese mental hospital with a largely untreated and idle patient population. Results demonstrated the remarkable effectiveness of this technique for motivating patients to resume productive activity. Interesting aspects of this treatment program, conducted under the difficulties of war-torn South Viet Nam, are described.
Atypical eruptive histiocytosis—a marker of underlying malignancy?
Authors: B. N. STATHAM, G. M. FAIRRIS, J. A. COTTERILL
Journal: British Journal of Dermatology (1984)

An eruptive histiocytosis was the presenting feature in a patient who developed acute myelomonocytic leukaemia. The differential diagnosis of the skin lesions is discussed and in the light of a recent similar report we propose that the two disorders are associated.
ANCA antigens, proteinase 3 and myeloperoxidase, are not expreΔed in endothelial cells
Authors: William F. Pendergraft, David A. Alcorta, Mårten Segelmark, Jia J. Yang, Robin Tuttle, J. Charles Jennette, Ronald J. Falk, Gloria A. Preston
Journal: Kidney International (2000)
Abstract 5133: Microtentacles and invadopodia: Functionally distinct plasma membrane protrusions of metastatic breast tumor cells separated mechanistically by c-Src
Authors: Eric M. Balzer, Rebecca Whipple-Bettes, Edward H. Cho, Michael A. Matrone, Susette C. Mueller, Stuart S. Martin
Journal: Cancer Research (2010)

Abstract During metastasis, invading cells produce various actin-based membrane protrusions that promote directional migration and proteolysis of extracellular matrix (ECM). Our recent discovery of a filamentous actin (F-actin) component within thin tubulin-based microtentacle (McTN) protrusions in suspended MDA-MB-231 tumor cells prompted an investigation of whether these extensions are structural or functional analogs of invadopodia. We show here that MDA-MB-231s are capable of producing invadopodia and McTNs, both of which contain F-actin. Invadopodium formation was enhanced by expression of a constitutively-active c-Src kinase, and repressed by expression of dominant negative, catalytically inactive form of c-Src. In contrast expression of inactive c-Src significantly increased the McTN formation. Additionally, direct inhibition of c-Src with the SU6656 inhibitor compound significantly enhanced McTN formation, suppressed the appearance of F-actin cores and phospho-cortactin foci, and completely blocked focal degradation of extracellular matrix. These results identify McTNs and invadopodia as separate structures that provide unique and sequential contributions to metastatic progression, namely endothelial attachment and invasion, respectively. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5133.
Performance of the Rheumatoid Arthritis Impact of Disease (RAID) score in relation to other patient-reported outcomes in a register of patients with rheumatoid arthritis
Authors: Turid Heiberg, Cathrine Austad, Tore K Kvien, Till Uhlig
Journal: Annals of the Rheumatic Diseases (2011)

Objective Delegates at the Outcome Measures in Rheumatology (OMERACT) 10 conference (Borneo, 4–8 May 2010) questioned how the new seven-domain Rheumatoid Arthritis Impact of Disease (RAID) score performs as a global measure. Score distributions and associations between the RAID score and patient-reported outcomes (PROs) and demographic variables were examined in a large sample of rheumatoid arthritis (RA) patients. Methods 1086 patients in the Oslo RA Register responded to a postal survey with commonly used PROs. Bivariate associations between the RAID score and other measures are reported as Pearson correlation coefficients. Results The mean RAID was 3.37±2.17. The distribution of the RAID score showed a slight floor effect: 17.5% had a score between 0 and 1, and 14.4% between 1 and 2, whereas only 1.0% and 0.3% had scores between 8 and 9, and 9 and 10, respectively. Correlations between the RAID score and the patient global assessment, Rheumatoid Arthritis Disease Activity Index, Short-Form (SF)-6D and EQ-5D were 0.82, 0.82, −0.77 and −0.73, respectively. Strong correlation was also seen between RAID and pain, the domain with highest weight, whereas correlations to measures of other RAID domains were moderate. The RAID score was higher in women than men (3.49 vs 2.95, p=0.001). Conclusion The RAID score was correlated more strongly to other global measures than to PROs, reflecting single health domains.