tbuckley/cabot-search huggingface dataset:
Risk Factors for Thromboembolism
Authors: Francesco Rodeghiero, Alberto Tosetto
Journal: Annals of Internal Medicine (1999)

Letters16 November 1999Risk Factors for ThromboembolismFrancesco Rodeghiero, MD and Alberto Tosetto, MDFrancesco Rodeghiero, MDS. Bortolo Hospital; 36100 Vicenza, Italy (Rodeghiero)S. Bortolo Hospital; 36100 Vicenza, Italy (Tosetto)Search for more papers by this author and Alberto Tosetto, MDS. Bortolo Hospital; 36100 Vicenza, Italy (Rodeghiero)S. Bortolo Hospital; 36100 Vicenza, Italy (Tosetto)Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-131-10-199911160-00018 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail IN RESPONSE:We thank Dr. Williams for his comment on our test for resistance to activated protein C. As he correctly points out, we measured resistance as originally described by Dahlback in 1993 (1). In fact, our investigation began just a few months before the factor V Leiden mutation was described, and we elected not to change our methods during the study. Dilution of plasma samples with factor V-deficient plasma is an efficient way to identify carriers of the factor V Leiden mutation (2), and many laboratories use this “improved” method to avoid the molecular test. Our findings clearly indicate ...References1. Dahlback B, Carlsson M, Svensson PJ. Familial thrombophilia due to a previously unrecognized mechanism characterized by poor anticoagulant response to activated protein C: prediction of a cofactor to activated protein C. Proc Natl Acad Sci U S A. 1993;90:1004-8. CrossrefMedlineGoogle Scholar2. Tosetto A, Rodeghiero F. Diagnosis of APC resistance in patients on oral anticoagulant therapy. Thromb Haemost. 1995;73:732-3. CrossrefMedlineGoogle Scholar3. Tosetto A, Missiaglia E, Gatto E, Rodeghiero F. The Vita Project: phenotypic resistance to activated protein C and FV Leiden mutation in the general population. Thromb Haemost. 1997;78:859-63. CrossrefMedlineGoogle Scholar Author, Article, and Disclosure InformationAuthors: Francesco Rodeghiero, MD; Alberto Tosetto, MDAffiliations: S. Bortolo Hospital; 36100 Vicenza, Italy (Rodeghiero)S. Bortolo Hospital; 36100 Vicenza, Italy (Tosetto) PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoActivated Protein C Resistance and Factor V Leiden Mutation Are Independent Risk Factors for Venous Thromboembolism Francesco Rodeghiero and Alberto Tosetto Risk Factors for Thromboembolism Eliot Williams Risk Factors for Thromboembolism Stephen J. Jay Metrics 16 November 1999Volume 131, Issue 10Page: 790-791KeywordsBlood plasmaFactor V LeidenMedical risk factorsPhenotypesProteinsThromboembolismThrombosisVenous thromboembolism ePublished: 15 August 2000 Issue Published: 16 November 1999 Copyright & PermissionsCopyright © 1999 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
miRNA-95 Mediates Radioresistance in Tumors by Targeting the Sphingolipid Phosphatase SGPP1
Authors: Xiaoyong Huang, Samira Taeb, Sahar Jahangiri, Urban Emmenegger, Elisa Tran, Jeff Bruce, Aruz Mesci, Elina Korpela, Danny Vesprini, C. Shun Wong, Robert G. Bristow, Fei-Fei Liu, Stanley K. Liu
Journal: Cancer Research (2013)

Radiation resistance poses a major clinical challenge in cancer treatment, but little is known about how microRNA (miR) may regulate this phenomenon. In this study, we used next-generation sequencing to perform an unbiased comparison of miR expression in PC3 prostate cancer cells rendered resistant to fractionated radiation treatment. One miR candidate found to be upregulated by ionizing radiation was miR-95, the enforced expression of which promoted radiation resistance in a variety of cancer cells. miR-95 overexpression recapitulated an aggressive phenotype including increased cellular proliferation, deregulated G2-M checkpoint following ionizing radiation, and increased invasive potential. Using combined in silico prediction and microarray expression analyses, we identified and validated the sphingolipid phosphatase SGPP1, an antagonist of sphingosine-1-phosphate signaling, as a target of miR-95 that promotes radiation resistance. Consistent with this finding, cell treatment with FTY720, a clinically approved small molecule inhibitor of S1P signaling, sensitized miR-95 overexpressing cells to radiation treatment. In vivo assays extended the significance of these results, showing that miR-95 overexpression increased tumor growth and resistance to radiation treatment in tumor xenografts. Furthermore, reduced tumor necrosis and increased cellular proliferation were seen after radiation treatment of miR-95 overexpressing tumors compared with control tumors. Finally, miR-95 expression was increased in human prostate and breast cancer specimens compared with normal tissue. Together, our work reveals miR-95 expression as a critical determinant of radiation resistance in cancer cells.
Endometrial Cancer in Relation to Patterns of Menopausal Estrogen Use
Authors: Noel S. Weiss
Journal: JAMA (1979)

Female residents of King County, Washington, in whom endometrial cancer developed between January 1975 and April 1976 were interviewed concerning prior use of menopausal estrogens. Their responses were compared with those of a random sample of women from the same population. Among current estrogen users, endometrial cancer risk was strongly related to duration of use; although only a minimal elevation of risk was present during the first two years, there was a rapid rise to a 20-fold excess after about ten to 15 years. Cessation of estrogen use led to a decline in incidence of endometrial cancer within several years, but the risk remained higher than in nonusers through the first decade after administration of the drug was stopped. Risk was elevated whether or not the regimen was cyclic and whether conjugated or other types of estrogens had been used. Dosages of less than 0.625 mg/day of conjugated estrogens produced a smaller increase in risk than did other dosages. (<i>JAMA</i>242:261-264, 1979)
Activity of [des-Aspartyl1]-Angiotensin II in Primary Aldosteronism
Authors: Robert M. Carey, Carlos R. Ayers, E. Darracott Vaughan, Michael J. Peach, Steven M. Herf
Journal: Journal of Clinical Investigation (1979)

This study describes the effects of [des-Aspartyl1]-angiotensin II ([des-Asp]-AII) on blood pressure and aldosterone production in patients with primary aldosteronism due to aldosterone-producing adrenal adenoma (APA) and idiopathic adrenal hyperplasia (IHA), and in normotensive control subjects. 10 patients with primary aldosteronism, 7 with APA and 3 with IHA, and 6 normotensive control subjects were placed on a constant 150-meq sodium diet for 4 days. [des-Asp]-AII was infused for 30 min at 6, 12, and 18 pmol/kg per min. Three groups of patients were identified on the basis of aldosterone response to [des-Asp]-AII. Group I, composed of normotensive control subjects, showed incremental increases in plasma aldosterone concentration from 6±1 to 14±3 ng/100 ml (P < 0.01) with [des-Asp]-AII infusion. Group II, composed of patients with primary aldosteronism, showed incremental increases in plasma aldosterone concentration from 33±8 to 65±13 ng/100 ml (P < 0.05) with 12 pmol/kg per min of [des-Asp]-AII. Group III, also composed of patients with primary aldosteronism, showed no increase of plasma aldosterone concentration with [des-Asp]-AII. Groups I and II showed similar percentage increases in plasma aldosterone concentration (P = NS). Group III showed significantly lower aldosterone responses than group I (P < 0.01). Group II included all patients with IHA and two patients with APA. Group III included only patients with APA. The blood pressure responses to [des-Asp]-AII of subjects in group I did not differ significantly from those of groups II or III.
The effects of endothelin-A receptor blockade during the progression of pacing-induced congestive heart failure
Authors: Daniel Saad, Rupak Mukherjee, Patrick B Thomas, Julie P Iannini, Charles G Basler, Latha Hebbar, Seung-Jun O, Suzanne Moreland, Maria L Webb, James R Powell, Francis G Spinale
Journal: Journal of the American College of Cardiology (1998)
Treatment of Malaria
Authors: George Watt
Journal: JAMA (1983)

<h3>To the Editor.—</h3> I refer to the recently published report of<i>Plasmodium falciparum</i>infection treated with exchange blood transfusion (1983; 249:244). Several aspects of the report deserve comment. Although there have been modifications in the treatment of the patient with severe malaria since this report was submitted, the principles have not changed. Attaining therapeutic serum levels of an appropriate antimalarial drug as quickly as possible during the critical first 24 hours seems to be the most important aspect of therapy. For a dangerously ill patient from a chloroquine-resistant area, the treatment of choice seems to be parenteral quinine sulfate, given as a loading dose of 20 mg/kg during four hours and then in maintenance dosages of 10 mg/kg three times daily.<sup>1</sup> With regard to prophylaxis, it is difficult to know what to recommend for someone going to eastern Thailand. There is not only chloroquine and pyrimethamine-sulfadoxine (Fansidar) resistance, but
Nucleic acid-based detection of influenza A virus subtypes H7 and N9 with a special emphasis on the avian H7N9 virus
Authors: D Kalthoff, J Bogs, T Harder, C Grund, A Pohlmann, M Beer, B Hoffmann
Journal: Eurosurveillance (2014)

In 2013, a novel influenza A virus of subtype H7N9 was transmitted from avian sources to humans in China, causing severe illness and substantial mortality. Rapid and sensitive diagnostic approaches are the basis of epidemiological studies and of utmost importance for the detection of infected humans and animals. We developed various quantitative reverse transcriptase PCR (RT-qPCR) assays for (i) the generic detection of the haemagglutinin (HA) gene of H7 viruses or the neuraminidase (NA) gene of N9 viruses, and (ii) the specific detection of HA and NA of the novel avian H7N9/2013 virus. The sensitivity of the newly developed assays was compared with previously published PCRs, and the specificity of all RT-qPCRs was examined using a panel of 42 different H7 and 16 different N9 isolates. Furthermore, we analysed the performance of the RT-qPCR assays with dilution series and diagnostic samples obtained from animal experiments. Our study provides a comprehensive set of RT-qPCR assays for the reliable detection of the novel avian H7N9 virus, with high sensitivity and improved and tailored specificity values compared with published assays. Finally, we also present data about the robustness of a duplex assay for the simultaneous detection of HA and NA of the avian influenza H7N9/2013 virus.
Selling the Medical Practice: The Physicians' and Surgeon's Guide
Authors: Alford C. Diller
Journal: JAMA (1992)

Medical Equity, a medical practice brokerage firm servicing clients all over the United States, was started in 1980. The authors have worked with this firm selling practices, solving practice management problems, and negotiating or arbitrating disputes and contracts. This book relates their experiences in selling the practices of these clients, fictionalized to maintain a confidential professional relationship. Formulas for determining sales price, explicit instructions for assessing the value of saleables, examples of complete practice sales contracts, step-by-step advice on how to negotiate and conclude an ethical and beneficial sale, and the data and method needed to make an informed decision to sell are basically what this book is all about. Medical Equity's fee for arranging a sale is 12% of the selling price, and the going rate in this market is 10% to 18%. This book is of value to any physicians who contemplate selling their practices. It will acquaint
Beneficial Effect of Intracoronary Verapamil on Microvascular and Myocardial Salvage in Patients With Acute Myocardial Infarction
Authors: Yoshiaki Taniyama, Hiroshi Ito, Katsuomi Iwakura, Tohru Masuyama, Masatsugu Hori, Shin Takiuchi, Nagahiro Nishikawa, Yorihiko Higashino, Kenshi Fujii, Takazo Minamino
Journal: Journal of the American College of Cardiology (1997)
Energy-related pollutants in the environment: Use of short-term tests for mutagenicity in the isolation and identification of biohazards
Authors: J. L. Epler, F. W. Larimer, T. K. Rao, C. E. Nix, T. Ho
Journal: Environmental Health Perspectives (1978)

In an effort to gather information on the potential genetic hazards of existing or proposed energy-generating or -conversion systems, we have begun a correlated analytical and genetic analysis of a number of technologies. The work is divided into two phases: one deals with known compounds expected to occur in the environment through energy production, conversion, or use; the other deals with actual samples from existing or experimental processes. To approach the problems of coping with and testing large numbers of compounds, we set up a form of the “tier system.” Operating units utilizing Salmonella, Escherichia coli, yeast, human leukocytes, mammalian cells, and Drosophila have been initiated. Various liquid-liquid extraction methods and column chromatographic separations have been applied to crude products and effluents from oil-shale, coal-liquefaction, and coal-gasification processes. Mutagenicity of the various fractions is assayed by means of reversion of histidine-requiring auxotrophs of Salmonella typhimurium; comparative studies are carried out with the other genetic systems. In order to incorporate metabolic activation of these fractions and compounds, rat liver homogenates (S-9) are used in the various assays. Results implicate chemicals occurring in the basic (ether-soluble) and the neutral fractions as potential genetic hazards. Chemical constituents of these fractions (identified or predicted) were tested individually for their mutagenic activity.
The Presence and Location of Glycogen in the Hypertrophied Right Ventricle of the Canine Heart
Authors: M. M. Laks, S. Van De Velde, H. J. C. Swan
Journal: Cardiovascular Research (1969)

After banding the pulmonary artery in the dog, the hypertrophied right ventricle contained glycogen adjacent to and sharply demarcated by the intercalated disc, and scattered throughout the myocytes. The glycogen was visible to a greater extent at the base than the apex, present in the beta form, and located between the myofibrils.
AUTO-INTOXICATION AND ALLIED INTESTINAL TROUBLESAS A POSSIBLE CAUSE OF CERTAIN VASCULAR AND FUNCTIONAL DISTURBANCES OF THE EYE
Authors: HIRAM WOODS
Journal: JAMA (1910)

This paper is the result of a personal conversation in October last, the participants being our Chairman and Secretary and myself.We met in Philadelphia, at the invitation of the Chairman, to discuss the program for this meeting.Among other matters, ocular disturbances from vari- ous toxic influences were considered.The papers which have already appeared were reviewed, and especially the pioneer work of de Schweinitz with his chemical helpers, Edsall and Fife.These researches, it will be recalled, had to do with inflammatory lesions, spe- cially those of the uveal tract.We thought of lid, con- junctival and various functional anomalies of the ciliary structure, things of daily observation, which resist local treatment, are prone to relapse, and which are not uncommonly associated with those remote symptoms which de Schweinitz has aptly defined as "conjectural."That is, one finds associated with certain lesions of the lid structures or functional derangement of the anterior uvea\p=m-\just the history and symptoms which, if asso- ciated with uvcitis, would lead one to think that the internist (having at command chemical means to inves- tigate defects of metabolism) could throw light on etiol- ogy.Is there more in this than association?If so, how far has the physiologic chemist enabled us to go?This question was put to Dr. Edsall, and he was invited to follow an interrogatory paper with one explaining what we want to' know.His reply was that he felt that, after his contributions, accessible to all, there was nothing more definite to add.We felt that it was useless to seek farther.Still, our own clinical observations led us to think the matter worth presenta¬ tion, and this position has been, since October, con¬ firmed by a paper by Dr. J. F. Shoemaker1 of
Biochemical effects of dietary intakes of different broccoli samples. I. Differential modulation of cytochrome P-450 activities in rat liver, kidney, and colon
Authors: Ole Vang, Henrik Frandsen, Kristian T. Hansen, J[oslash]rn N. S[oslash]rensen, Hilmer S[oslash]rensen, Ole Andersen
Journal: Metabolism (2001)
Cancer Cell Lines as Genetic Models of Their Parent Histology: Analyses Based on Array Comparative Genomic Hybridization
Authors: Joel Greshock, Katherine Nathanson, Anne-Marie Martin, Lin Zhang, George Coukos, Barbara L. Weber, Tal Z. Zaks
Journal: Cancer Research (2007)

Abstract Tumor-derived cell lines are used as in vitro cancer models, but their ability to accurately reflect the phenotype and genotype of the parental histology remains questionable, given the prevalence of documented cell line–specific cytogenetic changes. We have addressed the issue of whether copy number alterations seen in tumor-derived cell lines reflect those observed in studies of fresh tissue by carrying out a meta-analysis of array-based comparative genomic hybridization data that considers both copy number alteration frequencies and the occurrence of cancer gene amplifications and homozygous deletions. Pairwise correlation comparisons between the data sets of seven diagnosis-specific matched tumor and cell line groups indicate that the trends in aberration frequencies are highly correlated between tumors and cell line sets of matched cancer histology relative to unmatched pairings. Despite their similarities, cell lines showed uniformly higher locus-specific alteration frequencies (P = 0.004) and several recurring cell line–specific alterations emerged. These include the previously documented losses of 13q and 9p and gains of 20q, as well as additional undescribed cell line–specific gains of 5p, 7p, and 17q and losses of 18q and 4q. These results indicate that, on average, cell lines preserve in vitro the genetic aberrations that are unique to the parent histology from which they were derived while acquiring additional locus-specific alterations. These data may enable a more predictive understanding of individual cell lines as in vitro models of cancer biology and therapy. [Cancer Res 2007;67(8):3594–600]
Gyrate atrophy of the choroid and retina: amino acid metabolism and correction of hyperornithinemia with an arginine-deficient diet.
Authors: D Valle, M Walser, S W Brusilow, M Kaiser-Kupfer
Journal: Journal of Clinical Investigation (1980)

Four patients with gyrate atrophy of the choroid and retina were studied, all of whom exhibited the hyperornithinemia characteristic of this disorder. Elevated plasma histidine and diminished plasma lysine and branched-chain amino acids were also noted. The renal clearances of these four amino acids were not sufficiently elevated to explain their low plasma levels. In one subject, an arginine-deficient diet led to progressive reduction in plasma ornithine from 13 times normal to the upper limits of normal, along with the disappearance of ornithinuria and lysinuria. Orally administered alpha-aminoisobutyric acid facilitated the fall in plasma ornithine by increasing renal losses of ornithine. It also increased the clearances of most other amino acids. When plasma ornithine approached normal (less than 200 microM), plasma lysine became normal, plasma arginine became subnormal, and renal clearances of basic amino acids decreased. Long-term (1.5 yr) maintenance with a diet containing 10-20 g of protein plus essential amino acids served to keep plasma ornithine at between 55-355 microM; chorioretinal degeneration did not progress and vision apparently improved.
Blood platelet count and function are related to total and cardiovascular death in apparently healthy men.
Authors: E Thaulow, J Erikssen, L Sandvik, H Stormorken, P F Cohn
Journal: Circulation (1991)

Experimental animal and clinical studies indicate that blood platelets have an important role in atherosclerosis and formation of thrombi. Prospective studies presenting evidence of an association between blood platelet count and cardiovascular mortality have not been performed.From 1973 to 1975, blood platelets were counted, and their responsiveness to aggregating agents was studied in healthy middle-aged men. The aim was to assess the possible association between these variables and coronary heart disease. At 13.5 years of follow up, a significantly higher coronary heart disease mortality was observed among the 25% of subjects with the highest platelet counts. Platelet aggregation performed in a random subsample (150 of the 487 men), moreover, revealed that the 50% with the most rapid aggregation response after ADP stimulation had significantly increased coronary heart disease mortality compared with the others. These associations could not be explained by differences in age, lipids, blood pressure, or smoking habits.The present study is the first to present conclusive, prospective evidence of an association between platelet concentration and aggregability and long-term incidence of fatal coronary heart disease in a population of apparently healthy middle-aged men.
Electrocardiogram of the Healthy Adult Negro
Authors: CHARLES R. GREENE, JOHN J. KELLY
Journal: Circulation (1959)

The T waves of the precordial electrocardiograms are upright in healthy adult white subjects. On the other hand, several reports have drawn attention to T-wave inversion "juvenile pattern" in adult Negroes free of organic heart disease. This study demonstrated no increased incidence of the "juvenile pattern" in healthy adult Negroes. The standards for normality are identical for both white and Negro. When abnormalities of the T wave are found in Negroes, an explanation other than that of race should be sought.
Maternal plasma volume and disorders of pregnancy.
Authors: C W Redman
Journal: BMJ (1984)

Maternal plasma volume and disorders of pregnancyMaternal plasma volume increases progressively during the second and third trimesters of pregnancy.1 2 The extent of the increase depends on the size ofthe conceptus, tending to be most in women with multiple pregnancies,3 4 and least in women with babies small for gestational age.1 2 5 Maternal plasma volume