tbuckley/cabot-search huggingface dataset:
Abstract 2622: TMEFF2 inhibits human prostate cancer cell migration through its G protein activating domain.
Authors: Xiaofei Chen, C. Justus, L Yang, M.j. Ruiz-Echevarria
Journal: Cancer Research (2013)

Abstract The type I transmembrane protein with epidermal growth factor and two follistatin motifs 2 (TMEFF2) is expressed mainly in brain and prostate. Expression of TMEFF2 is deregulated in a significant fraction of primary and metastatic prostate, suggesting a role in this disease. In fact, we have previously shown that TMEFF2 can function as a tumor suppressor in vitro, inhibiting monolayer and anchorage-independent cellular growth in HEK293T cells and sarcosine-induced cell migration and invasion in benign prostate epithelial RWPE-1 cells. However, the molecular mechanisms involved in the tumor suppressor phenotype of TMEFF2 are not clear. TMEFF2 has several biologically important features. The extracellular domain contains two follistatin domains and an epidermal growth factor-like (EGF) domain, while the transmembrane domain and short cytoplasmic tail have features resembling a G protein activating domain, suggesting that it may signal through a G protein. We hypothesize that the tumor suppressor activity of TMEFF2 is, at least in part, due to signaling mediated by the putative G protein activating domain. In order to test this hypothesis, here we investigate the role of TMEFF2 and a mutant lacking the cytoplasmic tail on cellular migration of prostate cancer cells, and begin analyzing the molecular mechanisms involved in this effect. Our results demonstrate that overexpression of TMEFF2 in human prostate cancer cell line RWPE-2 causes about 60% decrease in cellular migration as assessed by wound-healing assays. Interestingly, we found that RWPE-2 cells overexpressing TMEFF2 have a defect in cell spreading on culture dishes (∼20% decrease) as well as on vitronectin-coated coverslips (∼30% decrease) and that this defect is accompanied by abolished focal adhesion and stress fiber formation. Moreover, the inhibition of TMEFF2 on cellular migration is dependent on its G protein activating domain as deletion of this domain rescues migration, spreading on vitronectin, and focal adhesion formation. The specific G protein pathway(s) involved in the anti-migratory effect of TMEFF2 is currently under investigation. In summary, the data presented here indicate, for the first time, that the anti-migratory effect of TMEFF2 in human prostate cancer cells is mediated by its G protein activating domain. These results raise the possibility that TMEFF2 agonists may be used as therapeutic agents to mediate tumor suppression via this pathway. Citation Format: Xiaofei Chen, C. Justus, L Yang, M.j. Ruiz-Echevarria. TMEFF2 inhibits human prostate cancer cell migration through its G protein activating domain. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2622. doi:10.1158/1538-7445.AM2013-2622
Epidemic of fractures during period of snow and ice.
Authors: Z A Ralis
Journal: BMJ (1981)

Characterizing the phenotypic heterogeneity in memory T cells generated following antigen encounter is a major challenge in immunology. Tools such as CyTOF flow cytometry or gene expression profiling of T cells can capture increasingly large numbers of parameters. However analysis of high dimensional data with standard techniques such as principal component analysis or hierarchical clustering often fails to capture the biological basis for observed phenotypic heterogeneity. We address this problem by applying a metagene projection strategy based on independent component analysis and consensus non-negative matrix factorization clustering. This approach assumes that the gene expression profile of a population of CD8+ T cells is generated by groups of genes that are representative of different biological processes, and deconvolves the data into individual components (or metagenes) that together comprise some or all of the overall "signal" in the data-space. The metagene projection strategy is able to provide a robust low dimensional description of transcriptional (or other complex) data based on groups of genes that represent known and novel biological mechanisms. It is also able to provide sensitive clustering and classification solutions. We illustrate the ability of this metagene projection strategy to capture novel molecular patterns in T cell differentiation states common to antigen-specific CD8+ T cells responses to HIV, HCV and metastatic melanoma.
Stone in the Cystic Duct Hepatobiliary Scintigraphic Findings
Authors: BELUR CHANDRAMOULY, VASUDEV BHIDE, LINDA SINGLETARY
Journal: Clinical Nuclear Medicine (1995)

Cholescintigraphy is a useful screening procedure for imaging hepatobiliary system in patients with right upper quadrant abdominal pain. The classic finding of cystic duct obstruction is nonvisualizatlon of the gallbladder over 4 hours. The authors report a study with visualization of cystic duct, proximal to the obstruction by a stone, as seen on the ultrasound study and proven at surgery.
The New Informants: The Betrayal of Confidentiality in Psychoanalysis and Psychotherapy
Authors: Mark H. Fleisher
Journal: JAMA (1996)

<i>The New Informants</i>by Bollas and Sundelson is a difficult book to review on several levels. There are some very strong criticisms made of the weakening of confidentiality by the courts, by some therapists themselves, and by third parties who may hold estate executor functions. These criticisms are easy to support. In the end the authors also provide a solution to the problem they define. As a biologically oriented psychiatrist who utilizes some psychotherapeutic techniques and has some involvement in managed care, I was challenged by this book to examine the issues involved. On the other hand the authors' perspectives are so shaped by their adherence to an orthodox psychoanalysis that it is difficult to contemplate their thesis. Their expressed regret for the lost esteem and financial support of their clinical practice in contemporary therapy is so predominant that the message on confidentiality is blurred. The idea that psychoanalysis, a
Robert Edward Duncan Williams
Authors: John Blandy, Bryony Williams
Journal: BMJ (2006)

Founder epidemiologist of the Framingham heart studyIn 1945 the Western world emerged from the second world war, only to be confronted by an epidemic of heart disease.The US government responded by setting up a study to identify the causes and to diagnose and treat early disease, though the latter aim was dropped as there was no treatment available.The town of Framingham in Massachusetts was chosen as the site for this study because it had a stable population of 28 000 and was close to Harvard University.The Framingham study, as it was known, became world famous."Along with Ancel Keys's seven country study, this was the work on which all other epidemiology of heart disease stands.There isn't a study that doesn't owe its origin to Framingham,"
This is Medical Progress?
Authors: Henry E. Simmons
Journal: JAMA (1974)

SINCE the first antibiotic, penicillin G, was marketed in the United States in 1943, these useful and potent antimicrobial agents have been widely adopted and used in the ensuing 30 years. The purpose of this article is to review the trends of antibiotic usage in the United States and try to assess the consequences of the remarkable popularity of this class of drugs. While the value and medical usefulness of appropriate antibiotic employment in clinical practice is unquestioned, there has been a considerable controversy over both the type and extent of antibiotic prescribing and the proper indications for use. Furthermore, the ecologic consequences of this wide usage are still unclear. The following are some of the issues: Has the wide use of antibiotics led to the emergence of new resistant bacterial strains? Has the ecology of "natural" or "hospital" bacterial flora been shifted because of antibiotic use? Has the ecology of
Hypopharyngeal injury caused by overtube insertion during endoscopic submucosal dissection
Authors: N. Muguruma, S. Okamura, Y. Imoto, K. Okamoto, T. Sueuchi, S. Kitamura, T. Kimura, T. Takayama
Journal: Endoscopy (2012)

A 46-year-old man with a gastric lesion was referred to our hospital. Esophagogastroduodenoscopy identified a flat lesion approximately 15 mm in diameter in the gastric body. Biopsies were taken but a final diagnosis could not be reached because of the small size of the specimens. Endoscopic submucosal dissection (ESD) was performed to remove and retrieve the whole lesion and obtain a precise diagnosis. To enable the scope to be inserted and retrieved repeatedly, an overtube was advanced after the endoscope had been inserted into the stomach. During the procedure, the operator felt a strong resistance and immediately aborted the overtube placement.
Is coeliac disease underdiagnosed?
Authors: E Rifkind, A Busuttil, A Ferguson
Journal: BMJ (1980)

the Birmingham findings.The overall pneumonia rate in Birmingham was higher (400h) than in Glasgow (26%°), where pneumonia was taken to mean either consolidation on chest radiograph or a chest infection which in the judgment of the attending physician required antibiotics.It would be interesting to know -what "confirmation" by chest radiography means in the Birminghamstudy, since the difference may be explained by differing definitions.We have been reluctant to draw definite conclusions from the complication rate in those children immunised against pertussis compared with those not immunised, since the numbers are small, and this may also apply to the Birmingham study.However, overall findings are similar, confirming that whooping cough still causes substantial numbers of hospital.admissions for a prolonged and unpleasant illness and that immunisation appears to protect children from being -admitted to hospital with the disease, either by preventing it altogether or by preventing serious complicatioris.
Inhibition of Adenosine-induced Coronary Vasodilatation
Authors: W. G. Nayler, J. M. Price, T. E. Lowe
Journal: Cardiovascular Research (1967)

Isolated spontaneously beating rat and guinea-pig hearts were used to investigate the direct dilator action of adenosine (5 μg./ml.) on the coronary vasculature. Phenoxybenzamine (10 μg./ml.) blocked the coronary dilator effect of adenosine and of another coronary dilator drug, dipyridamole, but failed to block adenosine-induced bradycardia. These results are interpreted to mean that adrenergic receptors may be involved in the coronary-dilator effect of adenosine.
ECTOPIC PREGNANCY: ITS COMPARATIVE SYMPTOMATOLOGY AND TREATMENT.Read before the Section of Obstetrics and Diseases of Women at the Forty-fourth Annual Meeting of the American Medical Association.
Authors: JOSEPH HOFFMAN
Journal: JAMA (1893)

long one but the patient left the table in good condition' and two hours later had a pulse of 80. On examining the mass after its removal a full formed eight pound child was found in its interior.The sac surrounding the child had compressed it until the anatomical landmarks used in diag¬ nosing pregnancy by palpation were completely obliterated -every nook and corner of the sac was filled with baby and placenta.The placenta!site was at the top of the sac, the cord down over the left eye of the child.The face of the child was turned to the mother's right side, the left shoulder and side of the face were the pelvic presenting parts.The
Penetration by a Giant Gastric Ulcer Induced by a Nonsteroidal Anti-Inflammatory Drug
Authors: I. Shimizu, T. Horie, H. Inoue, T. Oshima, M. Fujimoto, Y. Ozaki, K. Yamamoto, A. Iuchi, S. Ito
Journal: Endoscopy (2000)

A patient presented with penetration by a giant gastric ulcer resulting from treatment with a nonsteroidal anti-inflammatory drug. A test for Helicobacter pylori proved negative. Treatment with a combination of an inhibitor of gastric acid secretion and prostaglandin substitution therapy with misoprostol resulted in closure of the perforation and cicatrization of the gastric ulcer without the need for laparotomy.
Avian flu returns to Hong Kong
Authors: J. McManus
Journal: BMJ (1999)

Health officials in Hong Kong have reported two cases of a new strain of an avian influenza virus in humans. It is the first time that the new strain, H9N2, has been isolated from humans in Hong Kong, said Dr Paul Saw, deputy director of the city's department of health, adding that it had been found in ducks and chickens for many years.

According to a statement issued by …
Papaverine in Traumatic Arterial Spasm after Supracondylar Fracture of Humerus
Authors: P. Abbey
Journal: BMJ (1955)

PNEUMATOSIS CYSTOIDES MEDBRIIALThere seems little doubt that violent changes of intraabdominal pressure caused by vomiting have some con- nexion with the production of this condition.Vomiting so often occurs in association with chronic malnutrition that a nutritional factor may also be operative.
Paraneoplastic erythrocytosis associated with an inactivating point mutation of the von Hippel-Lindau gene in a renal cell carcinoma
Authors: Michael S. Wiesener, Melchior Seyfarth, Christina Warnecke, Jan Steffen Jürgensen, Christian Rosenberger, Neil V. Morgan, Eamonn R. Maher, Ulrich Frei, Kai-Uwe Eckardt
Journal: Blood (2002)
Developing a research and development strategy for primary care.
Authors: A Harris
Journal: BMJ (1993)

General practice research has been a minority activity and underfunded in the past. The creation of the purchaser and provider split, the introduction of medical audit, and the new research and development strategy for the NHS provide an opportunity to focus research on the health needs of the population. FHSAs, with the regional health authority, should develop a local strategy for research and development and appoint a lead officer, who may be the medical adviser. When negotiating contracts FHSAs need to back up their arguments with research evidence. NHS development research should cover quality, distribution, accessibility, outcome, and effectiveness. FHSAs should play a part in disseminating knowledge in the interests of achieving an effective and high quality service. GPs should be encouraged to participate in research by relaxing the regulations of compulsory hours of patient service and by creating a practice development allowance.
Tumor-Induced Tolerance and Immune Suppression Depend on the C/EBPβ Transcription Factor
Authors: Ilaria Marigo, Erika Bosio, Samantha Solito, Circe Mesa, Audry Fernandez, Luigi Dolcetti, Stefano Ugel, Nada Sonda, Silvio Bicciato, Erika Falisi, Fiorella Calabrese, Giuseppe Basso, Paola Zanovello, Emanuele Cozzi, Susanna Mandruzzato, Vincenzo Bronte
Journal: Immunity (2010)
Intestinal Bypass for Obesity
Authors: Ralph G. Wieland
Journal: JAMA (1982)

<h3>To the Editor.—</h3> The article by Edward D. Kalat, MD, and David B. Martin, MD (1981;246:982), suggested that a granulomatous reaction in the liver after jejunoileal bypass was favorably influenced by reanastomosis. The accompanying editorial supported this interpretation by stating that "the granulomatous nontuberculous disorder of the liver after J-I [jejunoileal] bypass presumably resolves with restoration of bowel continuity and weight gain." Our recently published article<sup>1</sup>consisting of an evaluation of 30 persons (from a group totaling 84 patients) on whom 101 liver biopsies were obtained at surgery and from three to nine years after jejunoileal bypass would not support the need for reanastomosis. In this study granulomas identical to those found by Drs Kalat and Martin were found initially on four biopsy samples and were not found in the later biopsy specimens, while two patients had granulomas on follow-up biopsies alone, with mild inflammation observed in all cases. Thus,
Severe bleeding disorders in children with normal coagulation screening tests
Authors: P Darbyshire, O B Eden
Journal: BMJ (1982)

treatment it could mean that the patient was receiving months of unnecessary, ineffective, and expensive treatment.Ultrasound is likely to prove useful in detecting spontaneous gall-stone recurrence and in monitor- ing the efficacy of postdissolution treatments given in attempts to prevent recurrence.If recurrent stones are detected, however, and treatment options include the possibility of further dissolution therapy, supplementary oral cholecystography will again be necessary.In our present state of ignorance, we agree that it seems sensible to use both cholecysto- graphy and ultrasonography of the gall bladder in monitoring the response to medical treatment and in the follow-up of patients after "confirmed" complete gall-stone dissolution.Indeed, this has been our policy since reliable ultrasonography first became available at Guy's Hospital.The routine use of both techniques is also part of the protocol of the British National Gall-stone Group in their multicentre, postdissolution trial.But to return to Dr Somerville and colleagues' title question: are we being misled?We do not think so, but we do not know and until the incidence of false-positive and false-negative results with both imaging techniques be- comes established, we believe that we should keep an open mind and avoid dogma.
Beneficial effects of danazol on symptoms and laboratory changes in cholinergic urticaria
Authors: ELIZABETH WONG, N. EFTEKHARI, M.W. GREAVES, A. MILFORD WARD
Journal: British Journal of Dermatology (1987)

In a double-blind placebo-controlled study, danazol, an attenuated androgen, was effective in reducing exercise-induced weals in cholinergic urticaria. Danazol treatment also caused significant elevations of several protease inhibitors, particularly antichymotrypsin which has been reported previously to be low in cholinergic urticaria.
Health Care in World Cities: New York, Paris, and London
Authors: David Chinitz
Journal: JAMA (2011)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Oral immunisation against typhoid fever in Indonesia with Ty21a vaccine
Authors: C.H. Simanjuntak, H. Totosudirjo, P. Haryanto, E. Suprijanto, F.P. Paleologo, N.H. Punjabi, N.D. Witham, R. Darmowigoto, None Soeprawoto, S.L. Hoffman
Journal: The Lancet (1991)
Evidence for Circulating Immune Complexes in Thyroid Disease
Authors: E. A. Calder, W. J. Penhale, E. W. Barnes, W. J. Irvine
Journal: BMJ (1974)

Anti-complementary activity was detected in the sera of 23 (59%) out of 39 patients with Hashimoto thyroiditis; in 10 (29%) out of 34 patients with primary hypothyroidism; in six (17%) out of 36 patients with thyrotoxicosis; and in only six (8%) out of 78 control subjects. Fractionation of Hashimoto sera by Sephadex G-200 chromatography showed that the anti-complementary activity was founding fractions of larger molecular size than monomeric IgG, being localized predominantly in the ascending limb of the second elution peak, suggesting that it was in the form of small immune complexes.
Morphological Studies in Tricuspid Atresia
Authors: BARBARA GULLER, JACK L. TITUS
Journal: Circulation (1968)

Fourteen hearts with tricuspid atresia were reviewed at autopsy. The study included examples of normally related great arteries and of transposition of the great arteries; instances of pulmonary stenosis were present in both groups. In these examples of tricuspid atresia the size of the right ventricle depended on the size of the ventricular septal defect. The ventricular septal defect and the right ventricle usually were smaller in proportion to the size of the heart in specimens with normally related great arteries. In the absence of transposition of the great arteries, the ventricular septal defect was found to be the site of obstruction to pulmonary flow, and its size in relation to the size of the aorta determined the size of the pulmonary artery. Morphologic abnormalities exist in the region in which the proximal portions of the major atrioventricular conduction system (A-V node, bundle of His, origin of bundle branches) normally are located; thus, anatomic abnormalities in the course of the atrioventricular conduction tissue could be expected in tricuspid atresia. Based on histologic evidence, severe elevation of pulmonary vascular resistance, in general, does not occur in infancy in tricuspid atresia.
In vivo analysis of lung cancer metabolism: nothing like the real thing
Authors: Christopher T. Hensley, Ralph J. DeBerardinis
Journal: Journal of Clinical Investigation (2015)

Cancer cells exhibit altered metabolism compared with that of the surrounding tissue. There is hope that these reprogrammed metabolic pathways in tumors hold the key to advances for both cancer imaging and therapy. Translation of observations in cultured cancer cells to live tumors, however, has proven to be highly complex, and robust methods to analyze metabolic activity in primary human tumors are sorely needed. In this issue of the JCI, Sellers et al. use perioperative administration of isotope-labeled glucose to lung cancer patients to differentiate metabolic pathways between tumors and benign lung. They identify pyruvate carboxylation, a reaction that enables glucose-derived carbon to replenish TCA cycle intermediates, as a key component of anabolic metabolism in tumor cells.
Lung Adenocarcinoma with Concurrent Exon 19 EGFR Mutation and ALK Rearrangement Responding to Erlotinib
Authors: Sanjay Popat, Alexandra Vieira de Araújo, Toon Min, John Swansbury, Melissa Dainton, Andrew Wotherspoon, Eric Lim, Andrew G. Nicholson, Mary E.R. O'Brien
Journal: Journal of Thoracic Oncology (2011)
Peritoneal adhesions: Facing the enemy
Authors: Emre Ergul, Birol Korukluoglu
Journal: International Journal of Surgery (2007)

Postsurgical adhesions severely affect the quality of life of millions of people worldwide. Numerous attempts have been made to prevent or reduce the incidence of peritoneal adhesions, but with limited success. An extensive Medline search, textbooks, scientific reports and scientific journals are the data sources. We also reviewed reference lists in all articles retrieved in the search as well as those of major texts regarding postsurgical intraperitoneal adhesion formation. A multifactorial approach including minimizing tissue injury, prophylactic antibiotic usage to reduce infectious morbidity, and biochemical agents with or without biomechanical barriers will reduce the amount and severity of adhesions. However, further research is needed to establish the safety, effectiveness and also the cost/benefit ratio of these substances in human subjects.