tbuckley/cabot-search huggingface dataset:
Abstract LB-213: Acheron augments triple negative breast cancer stem-like cells and exacerbates chemotherapeutic and radiation resistance
Authors: Steve J. Scully, Shannon Compton, Ralph A. Francescone, Lawrence Schwartz, Wei Yan, Rong Shao
Journal: Cancer Research (2011)

Abstract Basal-like breast cancers that are negative for clinically important receptors (estrogen, progesterone, and Her2/neu), carry the worst prognosis for patients that are diagnosed with breast cancer. Acheron is a novel protein that was recently demonstrated to be highly expressed in triple-negative basal-like breast cancers. Previous work in our lab has demonstrated that breast cancer cell lines that are engineered to express ectopic Acheron are more invasive, have higher levels of MMP-9 and VEGF, and develop increased tumor burden in vivo in xenografted SCID/beige mice. In order to better understand the role Acheron may play in these basal-like breast cancers, the triple negative breast cancer cell lines MDA 231 and MDA 435 were engineered to express ectopic Acheron. Engineered cells were cultured in serum free media with EGF and FGF to cultivate them as mammary cancer stem-like cells. Relative to empty vector control cells, Acheron-expressing MDA 435 cells demonstrated a greater ability to form acini-like structures in suspension as well as support anchorage independent growth. Cells that were cultivated in basal media with serum were also analyzed for cell adhesion and expression of the laminin adhesion protein CD29 (Beta-I Integrin). Expression of Acheron in either MDA 231 and 435 cells lead to a marked reduction in substrate adhesion on laminin-coated plates relative to controls. Consistent with this observation, Acheron-expressing cells also expressed reduced levels of CD29. FACS analysis for the apoptosis markers Annexin-V binding and propidium iodide exclusion demonstrated that Acheron-expressing cells were 2–3 times less sensitive to the same concentration of the chemotherapeutic drugs: Doxorubicin and docetaxel, and to a lesser extent to ionizing radiation (1.6 fold). In summary, we have extended the analysis of Acheron action in triple-negative basal-like breast cancer cells. We have found that these cells that express ectopic Acheron are more resistant to chemotherapeutic drugs and radiation and have a greater capacity to grow in an anchorage-independent manner. These data support the hypothesis that Acheron expression in triple-negative breast cancers may confer properties that would be anticipated to result in a poorer prognosis. Therefore, Acheron deserves attention and further investigation as a new cancer target in triple-negative basal-like breast cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr LB-213. doi:10.1158/1538-7445.AM2011-LB-213
Phase I/II clinical trial of anti-OX40, radiation and cyclophosphamide in patients with prostate cancer: immunological analysis
Authors: Magdalena Kovacsovics-Bankowski, Lana Chisholm, Jonna Vercellini, Marka Crittenden, Scot Lary, Brendan Curti, Andrew Weinberg
Journal: Journal for ImmunoTherapy of Cancer (2013)

Meeting abstracts OX40, a member of the Tumor Necrosis Factor Receptor superfamily is a potent co-stimulatory molecule. OX40 engagement increases T cell proliferation, effector function and survival. Pre-clinical studies have shown that OX40 agonist synergizes with radiation and cyclophosphamide to
Light-induced termination of spiral wave arrhythmias by optogenetic engineering of atrial cardiomyocytes
Authors: Brian O. Bingen, Marc C. Engels, Martin J. Schalij, Wanchana Jangsangthong, Zeinab Neshati, Iolanda Feola, Dirk L. Ypey, Saïd F.A. Askar, Alexander V. Panfilov, Daniël A. Pijnappels, Antoine A.F. de Vries
Journal: Cardiovascular Research (2014)

Atrial fibrillation (AF) is the most common cardiac arrhythmia and often involves reentrant electrical activation (e.g. spiral waves). Drug therapy for AF can have serious side effects including proarrhythmia, while electrical shock therapy is associated with discomfort and tissue damage. Hypothetically, forced expression and subsequent activation of light-gated cation channels in cardiomyocytes might deliver a depolarizing force sufficient for defibrillation, thereby circumventing the aforementioned drawbacks. We therefore investigated the feasibility of light-induced spiral wave termination through cardiac optogenetics. Neonatal rat atrial cardiomyocyte monolayers were transduced with lentiviral vectors encoding light-activated Ca2+-translocating channelrhodopsin (CatCh; LV.CatCh∼eYFP↑) or eYFP (LV.eYFP↑) as control, and burst-paced to induce spiral waves rotating around functional cores. Effects of CatCh activation on reentry were investigated by optical and multi-electrode array (MEA) mapping. Western blot analyses and immunocytology confirmed transgene expression. Brief blue light pulses (10 ms/470 nm) triggered action potentials only in LV.CatCh∼eYFP↑-transduced cultures, confirming functional CatCh-mediated current. Prolonged light pulses (500 ms) resulted in reentry termination in 100% of LV.CatCh∼eYFP↑-transduced cultures (n = 31) vs. 0% of LV.eYFP↑-transduced cultures (n = 11). Here, CatCh activation caused uniform depolarization, thereby decreasing overall excitability (MEA peak-to-peak amplitude decreased 251.3 ± 217.1 vs. 9.2 ± 9.5 μV in controls). Consequently, functional coresize increased and phase singularities (PSs) drifted, leading to reentry termination by PS–PS or PS–boundary collisions. This study shows that spiral waves in atrial cardiomyocyte monolayers can be terminated effectively by a light-induced depolarizing current, produced by the arrhythmogenic substrate itself, upon optogenetic engineering. These results provide proof-of-concept for shockless defibrillation.
Blood Pressure in the Unanesthetized Rat
Authors: FREDERICK OLMSTED, A. C. CORCORAN, IRVINE H. PAGE
Journal: Circulation (1951)

Heating is commonly used in indirect determinations of blood pressure in the rat. Its effect on arterial pressure is determined at short sequential intervals by a method which permits rapid measurements. Spontaneous variations in the rat's blood pressure are noted and analyzed. A plan is suggested which minimizes the effect of these variations by setting up criteria of significance of blood pressure change as between control and experimental periods.
Patient-Physician Interactions and Electronic Health Records
Authors: Daniel X. Yang
Journal: JAMA (2013)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
UK Health Commission downgrades four foundation trusts
Authors: Zosia Kmietowicz
Journal: BMJ (2004)

Four foundation hospital trusts have been graded down to two stars in this year's round of annual performance indicators from the healthcare watchdog, throwing into question their future as independent operators.

Peterborough Hospitals NHS Trust, Addenbrooke's NHS Trust, Moorfields Eye Hospital NHS Trust, and Papworth Hospital NHS Trust could face being stripped of their foundation status if they fail to satisfy demands from the independent regulator of NHS foundation trusts to explain the reasons for their demotion by 3 August.

Three …
Auditory Reading Matter For the Blind
Authors: C. Stanley Potter
Journal: JAMA (1969)

<h3>To the Editor:—</h3> I noticed the editorial entitled, "Better Braille for Blind Diabetics" (<b>208</b>:2330, 1969). While I find the material interesting and accurate, I am a little concerned with some of the things it leaves unsaid which I consider highly important. Most diabetics who lose their sight are adults by the time the disease manifests itself in the loss of visual perception. While some persons who become blind in adulthood do learn and use braille effectively, most do not regardless of the cause of blindness, because they prefer auditory reading systems which are widely available. Although it is entirely possible to provide a larger braille cell, the number of persons who would use it is not great. My interest in writing is to remind the physicians that auditory reading matter as well as substantial services designed to assist blind persons to develop compensatory skills and help accommodating attitudes are
Recognition and Killing of Brain Tumor Stem-Like Initiating Cells by CD8+ Cytolytic T Cells
Authors: Christine E. Brown, Renate Starr, Catalina Martinez, Brenda Aguilar, Massimo D'Apuzzo, Ivan Todorov, Chu-Chih Shih, Behnam Badie, Michael Hudecek, Stanley R. Riddell, Michael C. Jensen
Journal: Cancer Research (2009)

Solid tumors contain a subset of stem-like cells that are resistant to the cytotoxic effects of chemotherapy/radiotherapy, but their susceptibility to cytolytic T lymphocyte (CTL) effector mechanisms has not been well characterized. Using a panel of early-passage human brain tumor stem/initiating cell (BTSC) lines derived from high-grade gliomas, we show that BTSCs are subject to immunologic recognition and elimination by CD8(+) CTLs. Compared with serum-differentiated CD133(low) tumor cells and established glioma cell lines, BTSCs are equivalent with respect to expression levels of HLA class I and ICAM-1, similar in their ability to trigger degranulation and cytokine synthesis by antigen-specific CTLs, and equally susceptible to perforin-dependent CTL-mediated cytolysis. BTSCs are also competent in the processing and presentation of antigens as evidenced by the killing of these cells by CTL when antigen is endogenously expressed. Moreover, we show that CTLs can eliminate all BTSCs with tumor-initiating activity in an antigen-specific manner in vivo. Current models predict that curative therapies for many cancers will require the elimination of the stem/initiating population, and these studies lay the foundation for developing immunotherapeutic approaches to eradicate this tumor population.
Characterization of an autosomal dominant bleeding disorder caused by a thrombomodulin mutation
Authors: Yesim Dargaud, Jean Yves Scoazec, Simone J. H. Wielders, Christine Trzeciak, Tilman M. Hackeng, Claude Négrier, H. Coenraad Hemker, Theo Lindhout, Elisabetta Castoldi
Journal: Blood (2015)
Deregulating emergency contraception.
Authors: J O Drife
Journal: BMJ (1993)

Assessment three and a half years later has confirmed a structural as well as a functional benefit from the treatment, although a substantial group of children had severely impaired vision despite adequate cryotherapy.'5Earlier treatment with cryotherapy than that given in the American study has been advocated by some authors,'617 and this might further improve the results.Although cryotherapy is usually not associated with any problems, complications such as bradycardia, apnoea, chemosis, periorbital oedema, scarring, and late onset retinal detachment may occur.Another treatment of advanced retinopathy is laser treatment, which has recently been reported to be at least as effective as cryotherapy and may give rise to fewer systemic and ocular complications."8Finally, some cases, despite cryotherapy or laser treatment, progress to retinal traction and detachment and will need surgery with scleral buckling or vitrectomy.Although this form of surgery may produce good anatomical results, the fumctional results are still poor.920As cryotherapy substantially reduces the visual handicap of premature children, paediatric ophthalmologists should be responsible for identifying those who would benefit from it.
Bone marrow endothelial cells increase the invasiveness of human multiple myeloma cells through upregulation of MMP-9: evidence for a role of hepatocyte growth factor
Authors: I Vande Broek, K Asosingh, V Allegaert, X Leleu, T Facon, K Vanderkerken, B Van Camp, I Van Riet
Journal: Leukemia (2004)
The role of a purinergic P2z receptor in calcium-dependent cell killing of isolated rat hepatocytes by extracellular adenosine triphosphate
Authors: J P Zoetewij, B van de Water, H J de Bont, J F Nagelkerke
Journal: Hepatology (1996)

Extracellular adenosine triphosphate (ATP o) (0.4 mmol/L), a P2-purinergic receptor agonist, induces cytolysis in several cell types including isolated rat hepatocytes. In this study, we investigated the P2-receptor involved in ATP o-induced, Ca2+-dependent cytotoxicity in hepatocytes. Pretreatment of hepatocytes with oxidized ATP, a P2z-receptor antagonist, or complexation of ATP(4-) (the agonist for the P2z-receptor) with an excess of Mg2+, prevented ATP o-induced cell death. Both protective treatments also prevented the development of a sustained high intracellular Ca2+ concentration as well as the subsequent accumulation of inorganic phosphate (Pi). The P2Z-receptor agonist 3'-O-'(4-benzoylbenzoyl)-ATP (BzATP) was twofold more potent than ATP in eliciting cytolysis, which was preceded by a sustained high intracellular Ca2+ concentration; pretreatment with oxidized ATP prevented both the increase in the intracellular Ca2+ concentration and cell death. Prevention of ATP o-induced cell death, as well as the increases in the intracellular Ca2+ concentration and inorganic phosphate (Pi) was also achieved by decreasing the pH o to 6.9. Together the findings indicate that Ca2+-dependent cell killing by extracellular ATP in hepatocytes is mediated by a P2Z-receptor. The cytolytic effects correlated specifically with a secondary "late" increase in the intracellular Ca2+ concentration.
The normal trichogram of pubic hair
Authors: IGNACIO P.L. ASTORE, VICENTE PECORARO, ESTEBAN G. PECORARO
Journal: British Journal of Dermatology (1979)

The parameters of the trichogram of the pubic hair were studied with a previously described technique. Measurements were made on males and on non-pregnant, pregnant and post-partum females. In males and non-pregnant females denisty and rate of growth steadily decrease with age. There was a higher percentage of telogens in women. Hair thickness was not modified. The most important findings are related to pregnancy and post-partum which did not induce any change in the parameters of the trichogram of the public area. These findings indicate a behaviour of the pubic hair different from the scalp and axillary hair.
Molecular Imaging of Thrombus
Authors: Jonathan R. Lindner
Journal: Circulation (2012)

F or some time, scientists with expertise in medical imag- ing, cell biology, and chemical engineering have teamed together with the goal of producing targeted contrast agents for molecular imaging in cardiovascular disease.The goal of these efforts is to bring about techniques for noninvasively detecting and quantifying specific molecular processes that play a role in the pathophysiology of cardiovascular disease.Although there has been a tendency in the scientific literature to focus on how molecular imaging has been achieved, it is equally important to review why. Article see p 3117In the clinical setting, molecular imaging is likely to enhance and expand the diagnostic capabilities of current imaging applications.Nowhere has this been more successful than in cancer imaging, where targeted contrast agents can detect otherwise nonapparent primary or metastatic disease or be used to select appropriate therapies based on tumor or tumor microvascular phenotype. 1There are several areas of focus where molecular imaging could play an important role for early diagnosis and to guide patient management in cardiovascular disease.Imaging the cellular or molecular profile in atherosclerotic disease could yield important information on susceptibility for acute atherothrombotic complications. 2 In the future, it could also potentially be used to determine appropriateness for emerging therapies such as new drugs that interrupt the inflammatory response, which will likely be expensive and have adverse effects.Recent myocardial ischemia can be detected by molecular imaging of either a reduction in myocyte fatty acid utilization or postischemic inflammatory activation of the microvascular endothelium. 3,4This approach could be used for rapid diagnosis in patients with chest pain of unclear origin and allow spatial assessment of the postischemic region even hours after resolution.Large-scale clinical trials with imaging of labeled branched-chain fatty acids have already been completed showing that the technique can accelerate the diagnosis of acute coronary syndrome. 3Molecular imaging of sympathetic nerve activity could potentially be used to evaluate risk for life-threatening ventricular arrhythmia or adverse outcomes
The Effect of Digitalis on Cardiac Hypertrophy.
Authors: Frank B. Wynn
Journal: JAMA (1904)

consanguineous marriages have a tendency to produce mental unsoundness receives little support from the census figures.Visit of French Physicians to London.A visit of leading members of the French profession to Lon¬ don has been arranged, to include Professors Bouchard, Lucas- Champioiinière, Chauffard, Landouzy, Lermoyez, Marie and Sebileau.It is understood that they desire especially to ob¬ serve the system of clinical teaching and study in London.Visits to the Royal College of Surgeons, the Lister Institute, and the laboratories on the Embankment will be arranged.A banquet will be given to the visitors, and committees of enter¬ tainment and escort are appointed.
Obesity does not limit benefits of knee replacement, study shows
Authors: R. Dobson
Journal: BMJ (2008)

Clinically obese people can benefit almost as much as anyone else from knee replacement surgery, research has shown ( Annals of the Rheumatic Diseases 2008; doi 10.1136/ard.2008.093229).

The findings show that there is little justification for policies that deny this type of surgery to obese patients on the grounds of their body mass index (BMI). The researchers report that long term improvements in physical functioning in patients who had undergone knee replacement surgery extended to patients who were clinically obese, with a BMI greater than 30.

“There seems no justification for withholding total knee arthroplasty from obese patients solely …
Year 2000 Health Objectives Stress Early Detection and Intervention
Authors: A. Aubertin
Journal: JNCI Journal of the National Cancer Institute (1990)

Journal Article Year 2000 Health Objectives Stress Early Detection and Intervention Get access Amy Aubertin Amy Aubertin Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 82, Issue 19, 3 October 1990, Pages 1536–1537, https://doi.org/10.1093/jnci/82.19.1536 Published: 03 October 1990
Sexual Abuse of Children
Authors: Alvin A. Rosenfeld
Journal: JAMA (1978)

In recent weeks, the sexual abuse of children has received intensive attention in the media. To a large extent, this concern has centered around congressional hearings into child pornography. While we deplore the existence of this industry and the abuse of children that it entails, we believe that the use of children in pornography is but one tangential aspect of a more general problem—the sexual misuse of children. Numerous authors have become increasingly aware that incest, sexual relations between adults and children, and rape of children are important and not uncommon problems. Most work until now has tried to place blame on a responsible adult. Thus, these situations are often characterized simplistically as violent acts done by an adult perpetrator against an innocent child victim. While this approach is sensible if a person wishes to ascertain legal culpability, it ignores the fact that the psychological situation is far more complex.
TCT-714 Acute Hemodynamic Changes in the Immediate Phase Post Transcatheter Aortic Valve Replacement
Authors: Michalis Chrisoheris, Antonios Chalapas, Ioannis Paizis, Nikolaos Boumboulis, Antonis Ziakas, Stavros Chatzimitliadis, Ioulia Nikolaou, Stratis Pattakos, Konstantinos Spargias
Journal: Journal of the American College of Cardiology (2013)
1079-77 Late angiographic patency of the magnetic vascular port (MVP®) system for coronary artery anastomosis
Authors: Uwe Klima, Sven Martens, Gerhard Wimmer-Greinecker, Jan Gummert, Randall Wolf, Volkmar Falk, Ricardo A Costa, Alexandra J Lansky, Ecaterina Cristea, Mehran Roxana, Filip Casselman, Frank van Praet, Hugo Vanermen
Journal: Journal of the American College of Cardiology (2004)
Rx: A dose of ethics to revive trust in medical practice
Authors: A. Jain
Journal: BMJ (2013)

Gold jewellery? Cars and air conditioners to survive the sweltering Indian summer, or an exotic foreign trip to escape it? These are among the luxury gifts listed in a parliamentary committee report as being used by pharmaceutical companies to coax doctors into prescribing their drugs. In a BMJ feature on this topic (doi:10.1136/bmj.f2635), C M Gulati, editor of the Monthly Index of Medical Specialties , crisply lays out a 3C strategy employed by drug companies: “convince if possible, confuse if necessary, and corrupt if nothing else works.”

Medical representatives queuing up outside doctors’ clinics are a common sight in any Indian city or town. Doctors often rely on them for information on the latest drugs and devices in their specialty. In a relationship that acquires dimensions …
Aggregation of TDP-43 fragments triggers cellular aberrant function: implication in neurodegeneration
Authors: Meixia Che, Leilei Jiang, Chenjie Zhou, Yajun Jiang, Hongyu Hu
Journal: Molecular Neurodegeneration (2012)

TAR DNA binding protein of 43 kDa (TDP-43) is a nuclear factor functioning in RNA processing.It was also reported that TDP-43 aggregates are deposited in motor neurons implicated in the pathogenesis of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with ubiquitin (FTLD-U).To understand the mechanism underlying the inclusion body formation and possible functional alteration of TDP-43, we studied TDP-43 and its fragments and their effects on RNA processing in vitro and in cell models.We have identified a hydrophobic sequence in the C-terminus that is critical for the TDP-43 aggregation and inclusion formation.The synthetic peptide with this sequence forms an α-helical structure in solution but easily transforms into β-sheet structure.The inclusions formed by the C-terminal 35-kDa fragment (TDP-35) can recruit full-length TDP-43 to cytoplasmic deposition from functionally nuclear localization.TDP-35, rather than TDP-43 and the C-terminal 25-kDa fragment, is prone to aggregation in vitro, and it can further serve as a seed to facilitate aggregation of full-length TDP-43.This suggests that fragmentation of TDP-43 leads to cellular redistribution, inclusion body formation, and altered RNA processing, which are implicated in the molecular pathogenesis of ALS and FTLD.