tbuckley/cabot-search huggingface dataset:
Abstract 4521: Differential whole genome methylation profile in a responder and a non-responder to chemotherapy in colorectal cancer patients
Authors: Hassan Ashktorab, Rana Tbaishat, Hamed Rahi, Edward Lee, Duane T. Smoot, Olaide Ajayi, Xueguang Sun, Xi-Yu Jia, Hunter Chung, Eliza Bacon, Ron Leavitt, Hassan Brim
Journal: Cancer Research (2012)

Abstract Background: Colorectal adenoma (CRA) to cancer (CRC) progression involves in epigenetic changes including DNA methylation. Here we performed whole genome Bisulfite Next-Generation sequencing (WGBSNGS) on a normal, a tubular adenoma and a tumor patients’ tissue DNA to elucidate epigenetic drivers in normal to cancer progression. Patients and Methods: Genomic DNA was isolated from fresh frozen tissues from a patient with normal colon, from a tubular adenoma lesion (&amp;lt;0.5 cm) and from a carcinoma. A WGBSNGS at &amp;gt;20X depth was performed on these DNA samples., Alignment, mapping and CpG methylation analyses were done. Pyrosequencing and immunohistochemistry (IHC) was used to confirm selected CpG methylation. Results: We identified 94 unique CpG sites hypermethylated in 4 novel genes in adenoma compared to normal and 180 unique CpG sites in 7 genes in cancer. Ingenuity pathway analysis (IPA) showed that the methylated genes including a novel gene ATXN7L1 were involved in WNT pathway, ubiquotlation process and cross-talk with B-catenin, in the adenoma. Three of the hypermethylated genes in cancer were, Loc100506436: a novel gene with 17 unique CpG sites (EGFR pathway, cRNA CpG site hypermethylated is part of MEK1/2), GPNMB with 7 unique CpG sites (a G protein) and TNFAIP2 with 8 unique CpG sites (its role was noted in Head and neck cancer). IHC confirmed the lack of expression in cancer. Conclusion: This work provides insight into differential CpG island methylation profiles in CRA, CRC vs. normal colon tissue and provides a window into the more complex factors, including alteration of known and novel genes as a multiple CpG methylation drivers in colorectal carcinogenesis. Investigations into the possible roles of the novel gene targets in the context of early and prognostic methylation biomarker are underway. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4521. doi:1538-7445.AM2012-4521
Association of asynchronous protodiastolic segmental wall motion with impaired left ventricular relaxation.
Authors: P A Ludbrook, J D Byrne, A J Tiefenbrunn
Journal: Circulation (1981)

To determine if asynchronous segmental relaxation is associated with altered left ventricular (LV) diastolic function, we examined systolic and diastolic wall motion and function indexes in 16 patients without and 16 with asynchronous relaxation (groups 1 and 2, respectively). The segment with asynchronous relaxation was observed most frequently in the free anterior LV wall and was not consistently related geographically to coronary stenosis, nor to systolic asynergy in the same region, but was frequently accompanied by simultaneous segmental inward motion elsewhere in the ventricle. LV chamber volume stiffness during diastolic filling and at end-diastole was statistically similar in each group. Conversely, both T (58.3 +/- 2.3 msec vs 41.0 +/- 3.6 msec) and the isovolumic relaxation period (140.9 +/- 7.5 msec vs 116 +/- 6 msec) were significantly more prolonged, peak negative dP/dt was lower (1314 +/- 57 mm Hg/sec vs 1604 +/- 114 mm Hg/sec), and the y-axis intercept of the diastolic pressure-volume curve was higher in group 2 patients (7.96 +/- 0.98 mm Hg vs 4.88 +/- 0.93 mm Hg) (p less than 0.05 for each), indicating impaired relaxation and altered diastolic tone. With improved systolic function and relaxation properties after nitroglycerin, both the asynchronous outward relaxation and the inferior segment of simultaneous inward motion were ameliorated. Conversely, with increased ventricular preload and afterload induced by isometric exercise, both the asynchronous segmental outward motion anteriorly and the inward motion inferiorly with exaggerated. Asynchronous segmental relaxation may represent a compensatory mechanism in areas of normal contraction that offsets abnormal inward motion elsewhere, tending to maintain isovolumic status of the ventricle.
Terence Joseph Hypher
Authors: G M Jeremiah
Journal: BMJ (2004)

​FormerFormer consultant ophthalmic surgeon Swansea (b 1932; q Galway 1959, OStJ, FRCS Ed), d 1 March 2004.




Figure 1




Terence gained valuable early experience in eye surgery in Jordan, providing care to the Arab populations of the West Bank, Gaza, and East Jerusalem. He returned to the United Kingdom in 1966, and he was appointed consultant ophthalmologist at Singleton Hospital, Swansea, and Neath General Hospital, South Wales, in 1979. He helped introduce argon laser treatment for diabetic retinopathy, micro eye surgery with lens implantation, and ultrasonic investigation of eye disease. He retired in 1988 following a heart attack. During retirement he studied for a law degree, and took up learning the church organ. He leaves a wife, Valerie Jane, and three sons.
Lung Resection for Pulmonary Tuberculosis
Authors: B. J. Bickford, F. R. Edwards, J. R. Esplen, J. H. Gifford, A. M. Mair, O. F. Thomas
Journal: Thorax (1951)

In spite of the time and endeavour spent upon the treatment of pulmonary tuberculosis, it is far from the truth to say that the results of treatment are generally satisfactory.This statement is underlined by the figures of Thompson (1942Thompson ( , 1943)), who found that 42% of a group of 406 patients with a positive sputum in th-County of Durham were dead within 12 months of diagnosis, while only one patient in eight survived for 10 years.Cox (1936) and Bradbury (1946) give figures from the Lancashire County Council which showed somewhat similar results.Of 1,230 cases of pulmonary tuberculosis notified in 1930, 63.1% had died of their disease within five years, and of the 1,178 cases notified in 1940, 58.3% died in a similar period.When it is considered that 27.9% of the cases notified in 1930 and 33.6% of the cases notified in 1940 were still under treatment at the end of five years, the figures are discouraging indeed.Stocks and Lewis-Faning (1944) conclude that, in spite of a rapid fall in the number of notifications of disease in the period from 1923 to 1939, the average expectation of a patient eventually dying of his disease remained unchanged.It would not be fair to conclude from these figures that the prognosis of certain types of cases has not improved, and, in particular, the study of patients treated by thoracoplasty shows reason for optimism.Hurford (1941) published details concerning a group of 67 patients treated by thoracoplasty and a similar number who declined this treatment when it was offered.On follow-up for a minimum period of one year, 65% of operated cases were quiescent compared with 16% of the controls; only 18% of operated patients, but 45.5% of the controls, were dead.Sellors (1947) recorded sputum conversion in 84% of 633 patients treated by thoracoplasty between 1935 and 1946; 59.2% were fit for full work and 12.1 % were dead.Similar figures are given by other authors.Over a period of years certain views have been developed on the pathology and treatment of this disease which will be discussed, and as a development from these a more direct attack on the diseased area in the lung has been adopted which it is hoped may produce some improvement on these figures.on May 30,
Intracoronary adenosine potentiates coronary flow inhomogeneity produced by intravenous dobutamine between stenotic and adjacent normal coronary arteries
Authors: P. Petropoulakis, G. Pavlides, V. Vassilikos, A. Manginas, S. Papaloannou, D.V. Cokkinos
Journal: Journal of the American College of Cardiology (1998)
Association of Body Composition with Asthma Control in Preschool Children
Authors: T. Itazawa, Y. Adachi, Y.S. Adachi, Y. Ito, Y. Okabe, K. Yoshida, Y. Ohya, H. Odajima, A. Akasawa, T. Miyawaki
Journal: Journal of Allergy and Clinical Immunology (2011)
Monocytes From Peanut-Allergic Patients Express Higher Levels Of RALDH2 In Response To Peanut Protein Than Monocytes From Tolerant Subjects
Authors: Elizabeth Fleming, Bert Ruiter, Barry K. Hurlburt, Soheila J. Maleki, Wayne G. Shreffler
Journal: Journal of Allergy and Clinical Immunology (2014)
Individualized values for the disappearance rate parameter (Kd) in the enzymatic estimation of infarct size. A critique.
Authors: A S Slutsky
Journal: Circulation (1977)

A mathematical model to measure infarct size from serial estimates of CPK has been developed by Shell et al. Norris et al. have recently introduced a modification of this method by calculating an individualized disappearance rate parameter (Kd) from the uniformly monoexponential portion of the CPK vs time curve. This modification is based on the assumption that when CPK is decaying monoexponentially, the amount of CPK appearing in the circulation is zero. This paper presents a mathematical analysis which shows that the method of Norris et al. is theoretically unsound, as the CPK appearance function may be significantly different from zero and yet CPK vs time may still be monoexponential. Their method may lead to erroneous results in the calculation of infarct size.
Distribution of Norepinephrine in the Failing Bovine Heart
Authors: JOHN H. VOGEL, DAVID JACOBOWITZ, CHARLES A. CHIDSEY
Journal: Circulation Research (1969)

Studies were carried out during experimental heart failure induced in calves to compare the depletion of cardiac norepinephrine in the failing heart to the histochemical appearance of the adrenergic innervation in this tissue. A correlation has been established between the reduction of norepinephrine concentration and the changes in the distribution of the adrenergic neurotransmitter in the tissues. Absence of fluorescence in terminal varicose fibers in close association with cardiac muscle cells was characteristic of the failing heart. Little or no change occurred in preterminal fibers or in terminal fibers in connective tissue septa or around blood vessels. In two steers, recovery from heart failure was shown to be associated with a virtual restoration of the norepinephrine concentration and of thehistochemical appearance of the adrenergic nerve distribution in 28 days or less. These findings suggest that a reversible abnormality, possibly of neurotransmitter storage or synthesis, or both, is induced in the terminal portion of the cardiac adrenergic innervation as the result of the development of the failure state.
Increased Serum Creatinine Resulting from Hyperglycemia, Mimicking Transplant Rejection
Authors: ARTHUR J. MATAS
Journal: Annals of Internal Medicine (1975)

Letters1 October 1975Increased Serum Creatinine Resulting from Hyperglycemia, Mimicking Transplant RejectionARTHUR J. MATAS, M.D., RICHARD L. SIMMONS, M.D., FREDERICK C. GOETZ, M.D., CARL M. KJELLSTRAND, M.D., JOHN S. NAJARIAN, M.D.ARTHUR J. MATAS, M.D.Search for more papers by this author, RICHARD L. SIMMONS, M.D.Search for more papers by this author, FREDERICK C. GOETZ, M.D.Search for more papers by this author, CARL M. KJELLSTRAND, M.D.Search for more papers by this author, JOHN S. NAJARIAN, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-83-4-519 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptSerum creatinine elevation in a stable transplant recipient suggests rejection of the transplant and requires further evaluation and management. Eighty-seven patients with juvenile onset diabetes mellitus have received kidney transplants at the University of Minnesota between January 1968 and August 1974. In this patient-population, we have frequently observed significant elevations in serum creatinine levels (> 25%) in association with hyperglycemia. Correction of the hyperglycemia resulted in return of serum creatinine to normal levels without the use of antirejection therapy. Differentiation of these cases from true rejection cases is important in order to prevent unnecessary toxicity due to the therapy used...References1. QUAADE R: Undesirable effects of glucocorticoids. Acta Med Scand 500:77-80, 1969 Google Scholar2. Method N11, in Autoanalyzer Manual. Tarrytown, New York, Technicon Corp., 1962 Google Scholar3. WATKINS P: The effect of ketone bodies on the determination of creatinine. Clin Chem Acta 18:191-196, 1967 CrossrefMedlineGoogle Scholar4. LOEB J: The hyperosmolar state. N Engl J Med 290:1184-1187, 1974 CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Departments of Surgery and Medicine University of Minnesota Hospitals Minneapolis, Minnesota 55455 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByStatus of Renal Transplantation: A Clinical PerspectiveBeta 2-microglobulinaemia as index of diminishing renal function in diabetics.Beta2-microglobulinaemia as index of diminishing renal function in diabeticsHyperglycemia and Renal FunctionMICHAEL N. GOTTLIEB, M.D.Hyperglycemia and Rise in CreatinineBARBARA MCDOUGAL, M.D. 1 October 1975Volume 83, Issue 4Page: 519-520KeywordsCreatinineDiabetes mellitusHyperglycemiaRenal transplantationToxicityTransplant rejection Issue Published: 1 October 1975 PDF DownloadLoading ...
Abstract LB-417: Polymorphisms and haplotypes in <i>XRCC1</i> and risk of advanced non-small cell lung cancer
Authors: In-Suk Kim, Gyeong-Won Lee
Journal: Cancer Research (2010)

Abstract Background: Base excision repair (BER) is the primary DNA damage repair mechanism for repairing small base lesions resulting from oxidation and alkylation damage. Genetic variants in XRCC1, one of the BER genes, may be associated with smoking-related cancers. We tested the hypothesis that single nucleotide polymorphisms (SNPs) and/or hyplotypes in XRCC1 are related with the risk of non-small-cell lung cancer (NSCLC). Methods: We genotyped 3 known coding SNPs (Arg280His (rs25489), Arg399Gln (rs25487), and Gln632Gln (rs3547)) and 6 haplotype-tagging SNPs (htSNP) by Sequenom's (San Diego, CA, USA) high-throughput matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in 140 NSCLC patients and 217 controls from Korea. Analyses of individual SNPs and hyplotypes were performed using unconditional logistic regression adjusted for age, sex, and smoking status. Haplotypes were reconstructed according to the genotyping data and linkage disequilibrium status of these 9 SNPs using the Haploview version 4.1 software (Broad Institute of MIT and Harvard, Cambridge, MA, USA). Results: Minor allele heterozygotes of htSNP rs1799778 was increased risk of advanced NSCLC (adjusted OR, 2.118, 95% CI, 1.175-3.818, P= 0.013) and minor allele homozygote of htSNP rs2293036 was decreased risk of advanced NSCLC (adjusted OR, 0.363, 95% CI, 0.254-0.854, P = 0.020). Seven possible haplotypes were demonstrated in this study (Haplotype frequency &amp;gt;1%). The frequency of GGGGGGGGG haplotype composed of major alleles among selected all SNPs, was 0.109 and this haplotype showed protective effect of NSCLC risk (OR, 0.433; 95% CI, 0.246-0.740; P = 0.002). The association between the GGGGGGGGG haplotype and decreased risk of NSCLC was stable on permutation testing (P = 0.021). According to the smoking status, statistically significant associations between the rs1001581 polymorphisms and increased risk of NSCLC and the GGGGGGGGG haplotype and decreased risk of NSCLC risk were observed among never smokers (Adjusted P for trend = 0.005, P = 0.016), however, no association was noted among ever smokers. Conclusions: These findings suggest that the GGGGGGGGG haplotype composed of major alleles in all selected SNPs showed protective role against development of NSCLC and the rs1001581 polymorphisms showed increased risk of NSCLC in Koreans, especially nonsmokers, and smoking status modifies the associations between the XRCC1 genetic variants and NSCLC risk. Thus, this study presents several novel aspects on genetic susceptibility to NSCLC and larger studies of the role of the rs1001581 and haplotypes for NSCLC risk would be needed. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr LB-417.
Exposure of cotton workers in an experimental cardroom with reference to airborne endotoxins.
Authors: R Rylander, P Haglind
Journal: Environmental Health Perspectives (1986)

Workers from cotton mills were exposed to cotton dust during carding in an experimental cardroom. Cotton from different geographical locations with varying amounts of endotoxin were used. Exposure levels ranged from 0.6 to 3.6 mg dust/m3 (from a vertical elutriator) and from 0.1 to 8.0 micrograms/m3 of endotoxin. No relationship was found between the decrease in FEV1 over the workshift and the amount of airborne dust. Airborne endotoxin correlated with the decrease in FEV1 and the increase in blood neutrophils. The FEV1 decrease was more pronounced among smokers. The data suggest that the amount of airborne endotoxin determines the risk for development of the acute symptoms in the byssinosis syndrome.
Nitric oxide mediates the formation of synaptic connections in developing and regenerating olfactory receptor neurons
Authors: A. Jane Roskams, David S. Bredt, Ted M. Dawson, Gabriele V. Ronnett
Journal: Neuron (1994)

Nitric oxide (NO) is a diffusible free radical that functions as a second messenger and neurotransmitter. NO synthase (NOS) is highly and transiently expressed in neurons of the developing olfactory epithelium during migration and establishment of primary synapses in the olfactory bulb. NOS is first expressed at E11 in cells of the presumptive nervous layer of the olfactory placode. NOS immunoreactivity persists in the descendants of these cells that differentiate into embryonic olfactory receptor neurons (ORNs). Olfactory NOS expression in the ORN and in its afferents rapidly declines after birth and is undetectable by P7. Following bulbectomy, NOS expression is rapidly induced in the regenerating ORN and is particularly enriched in their outgrowing axons. Immunoblot and Northern blot analyses similarly demonstrate an induction of NOS protein and mRNA expression, respectively, the highest levels of which coincide with peaks of ORN regeneration. These data argue against a role for NO in odorant-sensitive signal transduction, but suggest a prominent function for NO in activity-dependent establishment of connections in both developing and regenerating olfactory neurons.
Percutaneous endocardial versus selective coronary venous cellular delivery: Comparisons of transplant efficiency, distribution, and efficacy in reducing infarct size and improving myocardial function
Authors: Erik T. Price, Fumiaki Ikeno, Ralph C. Fenn, Pauline Chu, Jennifer K. Lyons, Peter J. Fitzgerald, Alan C. Yeung, Paul G. Yock, Mehrdad Rezaee
Journal: Journal of the American College of Cardiology (2003)
The Properties of Immune Complexes Formed by Human Antibodies to Factor VIII
Authors: John Lazarchick, Leon W. Hoyer
Journal: Journal of Clinical Investigation (1977)

Although human antibodies to Factor VIII inactivate its procoagulant activity, they do not form immunoprecipitates when tested with this antigen. To understand this observation, we have examined the interaction of normal human Factor VIII with four high-titer human anti-Factor VIII, two from transfused hemophiliacs and two “spontaneous” antibodies from nonhemophilic individuals. An estimate of the size of complexes formed by these antibodies has been obtained by agarose gel filtration of mixtures of anti-Factor VIII with cryoprecipitate. Complexed anti-Factor VIII was detected by the method of Allain and Frommel: acid dissociation of complexes at pH 3.5.
Does obesity influence early target organ damage in hypertensive patients?
Authors: R E Schmieder, F H Messerli
Journal: Circulation (1993)

BACKGROUND Various prospective studies have found that lean hypertensive patients have greater cardiovascular morbidity and mortality than obese hypertensive subjects. It was therefore hypothesized that hypertension is more benign when associated with obesity. In the present study, we evaluated effects of obesity on early target organ damage in patients with essential hypertension. METHODS AND RESULTS In a total of 207 subjects, systemic and renal hemodynamics as well as left ventricular structure and function were assessed by measuring cardiac output (indocyanine green dye dilution), renal blood flow (clearance of 131I paraimmunohippuric acid), and mean arterial pressure (invasively) and by two-dimensionally guided M-mode echocardiographic findings. Systemic and renal vascular resistance, compliance of the large arteries evaluated by the stroke volume/pulse pressure index, and left ventricular mass served as parameters for early target organ damage. All individuals were categorized into four groups: lean and obese normotensive as well as lean and obese hypertensive subjects. In obese hypertensive patients, total peripheral resistance was significantly lower and stroke volume/pulse pressure index was higher than in the lean hypertensive group, almost reaching values of normotensive control subjects. No effect of obesity on the renal circulation was noted, whereas in hypertension, renal vascular resistance was elevated. The degree of left ventricular hypertrophy was more pronounced in the hypertensive groups than in their normotensive counterparts and progressively increased with obesity. Nevertheless, in obese hypertensive patients, left ventricular function, as measured by fractional fiber shortening and velocity of circumferential fiber shortening, was maintained despite the fact that the heart had been exposed to the double burden of an increased preload (obesity) and afterload (hypertension). CONCLUSIONS Obesity had a disparate effect on target organs in hypertension. At rest, obesity seemed to mitigate cardiovascular changes in the systemic vascular bed caused by hypertension. However, no such mitigation was observed in the renal vasculature, and left ventricular hypertrophy was even exacerbated by the presence of obesity. Our findings in part negate the concept that obesity is able to exert a protective effect on early target organ damage in hypertensive patients and, in particular, on the heart.
Ryanodine Receptor Oxidation Causes Intracellular Calcium Leak and Muscle Weakness in Aging
Authors: Daniel C. Andersson, Matthew J. Betzenhauser, Steven Reiken, Albano C. Meli, Alisa Umanskaya, Wenjun Xie, Takayuki Shiomi, Ran Zalk, Alain Lacampagne, Andrew R. Marks
Journal: Cell Metabolism (2011)