tbuckley/cabot-search huggingface dataset:
Affinity binding of antibodies to supermacroporous cryogel adsorbents with immobilized protein A for removal of anthrax toxin protective antigen
Authors: Ganesh C. Ingavle, Les W.J. Baillie, Yishan Zheng, Elzbieta K. Lis, Irina N. Savina, Carol A. Howell, Sergey V. Mikhalovsky, Susan R. Sandeman
Journal: Biomaterials (2015)

Polymeric cryogels are efficient carriers for the immobilization of biomolecules because of their unique macroporous structure, permeability, mechanical stability and different surface chemical functionalities. The aim of the study was to demonstrate the potential use of macroporous monolithic cryogels for biotoxin removal using anthrax toxin protective antigen (PA), the central cell-binding component of the anthrax exotoxins, and covalent immobilization of monoclonal antibodies. The affinity ligand (protein A) was chemically coupled to the reactive hydroxyl and epoxy-derivatized monolithic cryogels and the binding efficiencies of protein A, monoclonal antibodies to the cryogel column were determined. Our results show differences in the binding capacity of protein A as well as monoclonal antibodies to the cryogel adsorbents caused by ligand concentrations, physical properties and morphology of surface matrices. The cytotoxicity potential of the cryogels was determined by an in vitro viability assay using V79 lung fibroblast as a model cell and the results reveal that the cryogels are non-cytotoxic. Finally, the adsorptive capacities of PA from phosphate buffered saline (PBS) were evaluated towards a non-glycosylated, plant-derived human monoclonal antibody (PANG) and a glycosylated human monoclonal antibody (Valortim®), both of which were covalently attached via protein A immobilization. Optimal binding capacities of 108 and 117 mg/g of antibody to the adsorbent were observed for PANG attached poly(acrylamide-allyl glycidyl ether) [poly(AAm-AGE)] and Valortim® attached poly(AAm-AGE) cryogels, respectively, This indicated that glycosylation status of Valortim® antibody could significantly increase (8%) its binding capacity relative to the PANG antibody on poly(AAm-AGE)-protien-A column (p < 0.05). The amounts of PA which remained in the solution after passing PA spiked PBS through PANG or Valortim bound poly(AAm-AGE) cryogel were significantly (p < 0.05) decreased relative to the amount of PA remained in the solution after passing through unmodified as well as protein A modified poly(AAm-AGE) cryogel columns, indicates efficient PA removal from spiked PBS over 60 min of circulation. The high adsorption capacity towards anthrax toxin PA of the cryogel adsorbents indicated potential application of these materials for treatment of Bacillus anthracis infection.
DALTON study: A randomized study comparing outpatient dalteparin administration to inpatient heparin for the initiation of anticoagulation in atrial fibrillation
Authors: François Phillippon, Gilles E. O'Hara, Jean Champagne, Lyne Charbonneau, Hélène Villeneuve, Louis Blier, Franck Molin, Marcel Gilbert
Journal: Journal of the American College of Cardiology (2003)
Ethical Considerations in the Public Policy Laboratory
Authors: Matthew M. Davis
Journal: JAMA (2000)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract P6-02-01: Meta-Analysis of Ductal Carcinoma In Situ (DCIS) on Core Needle Biopsy — Underestimation and Predictors of Invasion
Authors: ME Brennan, RM Turner, S Ciatto, ML Marinovich, JR French, P Macaskill, H. Nehmat
Journal: Cancer Research (2010)

Abstract Background: A diagnosis of ductal carcinoma in situ (DCIS) on core needle biopsy (CNB) may represent an underestimate (or understaging) of invasive breast cancer (IBC). This is identified on excision histology in 4%-35% of CNB diagnoses of DCIS. It is of clinical significance as it may prevent informed discussion about management options for IBC and may mean that more than one surgical procedure is required. We systematically review the literature on CNB to report pooled estimates for underestimation of IBC following CNB diagnosis of DCIS and identify preoperative predictors of invasion. Methods: Studies were identified by searching MEDLINE and were evaluated against predetermined inclusion criteria: data were extracted independently by 2 authors. We calculated study-specific proportions for DCIS underestimates. Using meta-regression (random effects logistic models) we investigated the association between study-level preoperative variables and understaged IBC. Results: 47 studies met eligibility criteria reporting 6,213 cases of DCIS on CNB with excision histology as the reference standard. Table 1: Pooled underestimation rates for There were 1,482 underestimates (DCIS on CNB and IBC on excision); pooled estimate 25.9%(95% CI 22.2, 29.9). Preoperative variables associated with underestimation included ultrasound-guidance (vs stereotaxis), 14Gautomated (vs 11GVAB), high grade CNB lesion (vs nonhigh grade), size &amp;gt;20mm, BIRADS 4/5 (vs BIRADS3), mammographic mass (vs calc only) and palpability. Conclusion: Using available evidence we estimate that around 26% of CNB DCIS diagnoses represent understaged IBC. We identified preoperative variables associated with higher risk of understaging. This information can be used for preoperative discussion of management options, including possible sentinel node biopsy at the time of excision. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr P6-02-01.
Erratum: Corrigendum: Distinct H3F3A and H3F3B driver mutations define chondroblastoma and giant cell tumor of bone
Authors: Sam Behjati, Patrick S Tarpey, Nadège Presneau, Susanne Scheipl, Nischalan Pillay, Peter Van Loo, David C Wedge, Susanna L Cooke, Gunes Gundem, Helen Davies, Serena Nik-Zainal, Sancha Martin, Stuart McLaren, Victoria Goodie, Ben Robinson, Adam Butler, Jon W Teague, Dina Halai, Bhavisha Khatri, Ola Myklebost, Daniel Baumhoer, Gernot Jundt, Rifat Hamoudi, Roberto Tirabosco, M Fernanda Amary, P Andrew Futreal, Michael R Stratton, Peter J Campbell, Adrienne M Flanagan
Journal: Nature Genetics (2014)
Health in the Developing World
Authors: Mehdi Tavassoli
Journal: JAMA (1975)

To the Editor.—  Dr. Ekwo and I appear to agree that social development has lagged too far behind scientific progress. Scientific progress is, of course, an integral aspect of social development, and precisely for this reason, science must be responsive to the needs of society. One may accept that science leads society in a forward movement, but scientific progress cannot gallop too far ahead of social development, for when this occurs, the relevance of science to society is lost. Science is then isolated, functioning within a vacuum.
Live Attenuated Rubella Virus Vaccines Prepared in Duck Embryo Cell Culture
Authors: Eugene B. Buynak
Journal: JAMA (1968)

Merck strain rubella virus and Meyer-Parkman monkey kidney attenuated HPV-77 virus were prepared at various levels of attenuation for investigative use in humans and animals. The vaccines were produced in duck embryo cell culture and represented a spectrum which ranged from underattenuation, causing communicable mild rubella, to overattenuation with apparent nonexcretion and inadequate immunization. Merck strain rubella virus at attenuation level B, or possibly C, and Meyer-Parkman HPV-77 virus in fifth passage in duck embryo cell culture appear to present the attributes of an effective noncontagious vaccine. The antibody, produced in children after administration of level C vaccine, persisted undiminished for at least one year. Duck embryo cell culture appeared to be an attractive material for preparing vaccine because it was of a nonmammalian source and because of the relative freedom of ducks from disease.
Evidence for an antagonist form of the chemokine CXCL10 in patients chronically infected with HCV
Authors: Armanda Casrouge, Jérémie Decalf, Mina Ahloulay, Cyril Lababidi, Hala Mansour, Anaïs Vallet-Pichard, Vincent Mallet, Estelle Mottez, James Mapes, Arnaud Fontanet, Stanislas Pol, Matthew L. Albert
Journal: Journal of Clinical Investigation (2010)

Chronic infection with hepatitis C virus (HCV) is a major public health problem, with nearly 170 million infected individuals worldwide. Current treatment for chronic infection is a combination of pegylated IFN-α2 and ribavirin (RBV); however, this treatment is effective in fewer than 50% of patients infected with HCV genotype 1 or 4. Recent studies identified the chemokine CXCL10 (also known as IP-10) as an important negative prognostic biomarker. Given that CXCL10 mediates chemoattraction of activated lymphocytes, it is counterintuitive that this chemokine correlates with therapeutic nonresponsiveness. Herein, we offer new insight into this paradox and provide evidence that CXCL10 in the plasma of patients chronically infected with HCV exists in an antagonist form, due to in situ amino-terminal truncation of the protein. We further demonstrated that dipeptidyl peptidase IV (DPP4; also known as CD26), possibly in combination with other proteases, mediates the generation of the antagonist form(s) of CXCL10. These data offer what we believe to be the first evidence for CXCL10 antagonism in human disease and identify a possible factor contributing to the inability of patients to clear HCV.
Effect of amiodipine on exercise tolerance and quality of life in mild-to-moderate heart failure with systolic dysfunction
Authors: James E. Udelson, C.Andrew DeAbate, Nampalill Vijay, Steven Chrysant, Krishan Goyle, Irving Loh, Joel Sklar, William Smith, Marvin A. Konstam
Journal: Journal of the American College of Cardiology (1996)
Fear of Rejection — Monitoring the Heart-Transplant Recipient
Authors: John A. Jarcho
Journal: New England Journal of Medicine (2010)

Among patients undergoing heart transplantation, a fundamental clinical concern is the risk of rejection of the new organ. Although modern immunosuppressive regimens have reduced the incidence of rejection substantially, about one quarter of recipients will still have a rejection episode requiring treatment during the first year after transplantation.1 Acute rejection is the cause of 12% of deaths occurring between 1 month and 1 year after transplantation.1 There is no established noninvasive marker of rejection available for heart-transplant recipients; instead they must undergo serial endomyocardial biopsies with histologic evaluation of myocardial tissue to monitor for rejection. Endomyocardial biopsy is an invasive . . .
Don't Share Razors or Toothbrushes
Authors: Craig N. Shapiro
Journal: Annals of Internal Medicine (1994)

Letters15 July 1994Don't Share Razors or ToothbrushesCraig N. Shapiro, MDCraig N. Shapiro, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-121-2-199407150-00028 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail IN RESPONSE:The recommendations presented in the editorial specifically addressed counseling of patients known to be infected with one of the hepatitis viruses. Regarding general recommendations in households or institutions, I concur with Dr. Frothingham that sharing of razors and toothbrushes should be avoided, both to prevent blood exposure and to reduce the risk for transmission of hepatitis viruses and other blood-borne viruses [1].REFERENCE1. Simonds RJ, Rogers MF. HIV prevention—bringing the message home. N Engl J Med. 1993; 329:1883-5. Google Scholar Author, Article, and Disclosure InformationAuthors: Craig N. Shapiro, MD PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 15 July 1994Volume 121, Issue 2Page: 153-154KeywordsBloodHepatitis virusesInfectious hepatitis ePublished: 15 August 2000 Issue Published: 15 July 1994 Copyright & PermissionsCopyright © 1994 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Pleural Cerebrospinal Fluid Input and Output Kinetics Dynamically Demonstrated by In-111 DTPA Myelography in a Patient with Pleural Cerebrospinal Fluid Fistulae
Authors: Daisuke Maeda, Shigeru Kosuda, Shoichi Kusano, Yoshisuke Fujikawa
Journal: Clinical Nuclear Medicine (2004)

A 67-year-old man with thoracic spine metastasis from left renal cell carcinoma complained of dyspnea due to gradually developing bilateral pleural effusions after decompression, tumor excision, and anterior–posterior reconstruction with instrumentation and fusion from T12 to L2. Serial whole-body imaging after intrathecal injection of In-111 DTPA revealed pleural cerebrospinal fluid fistulae at the T12 level, and dynamically exhibited the input and output kinetics into the pleural spaces.
Identification of a lymphocyte surface receptor for low density lipoprotein inhibitor, an immunoregulatory species of normal human serum low density lipoprotein.
Authors: L K Curtiss, T S Edgington
Journal: Journal of Clinical Investigation (1978)

The present study demonstrates the existence on human peripheral blood lymphocytes of a saturable cell surface receptor for low density lipoprotein inhibitor (LDL-In), a subset of normal human serum low density lipoprotein (LDL) that has been previously demonstrated to suppress selected lymphocyte functions in vivo and in vitro. The binding of radioiodinated LDL-In of demonstrable biological activity occurs rapidly and is quantitatively augmented by prior cultivation of the lymphocytes in lipoprotein-depleted serum, suggesting regulation of receptor density by lipoproteins in vivo. Binding is temperature dependent, facilitated by calcium ions, saturable at 4 degrees C within 40-60 min, and blocked by prior exposure to unlabeled LDL-In. The lymphocyte receptor is trypsin sensitive and regenerates in vitro with a t1/2 of 3.6 h. LDL-In receptors are calculated to have a maximum density of 4,860 +/- 460 per cell if uniformly distributed on all lymphocyte subsets. These receptors have an estimated average association constant of 1.47 X 10(7) liters/mol. When considered in context of the estimated concentration of LDL-In in blood, the receptors should be partially occupied in vivo by endogenous plasma LDL-In. Prior site occupancy inhibition experiments designed to analyze the specificity of LDL-In binding demonstrate that (a) LDL-In is 13.7-fold more effective than whole LDL in blocking the subsequent binding of 125I-LDL-In to cells; and that (b) LDL is 11-fold more effective than LDL-In in blocking the binding of 125I-LKL. This is consistent with the degree of contamination of each lipoprotein with the other lipoprotein. An independent identity of the LDL-In receptor is also supported by observations that in contrast to the previously described LDL receptor, synthesis and expression of the LDL-In receptor on lymphocytes are not suppressed by cultivation of the cells in the presence of 25-hydroxycholesterol and cholesterol. These findings suggest the existence of a previously undescribed and discrete receptor on lymphocytes for LDL-In, and that the modulation of lymphocyte function by LDL-In may be mediated by a specific cell surface receptor pathway.
Medical Student Abuse
Authors: Donna A. Rosenberg
Journal: JAMA (1984)

IN A Jan 15, 1982, COMMENTARY in<i>The Journal of the American Medical Association</i>, Silver<sup>1</sup>remarked on the unfortunate transformation that took place in some medical students. These students, while eager and enthusiastic at the time of admission to medical school, became cynical, frightened, depressed, or frustrated men and women after they had been in medical school for a while. He speculated that the changes that occurred could, to a substantial degree, be the result of medical student abuse. He wondered how we might deal with medical student abuse. How should this problem be brought to the attention of the faculty? Would the faculty acknowledge or deny the possibility of abuse? Would they minimize the changes in the students by attributing them to a variety of other causes? Would some faculty members declare that it was inevitable for medical students to feel as they did? Was the abuse, when it
Military Makes 'House Calls' in 100 Countries
Authors: Phil Gunby
Journal: JAMA (1997)

ABSTRACT MILITARY MEDICAL personnel of the US Air Force, Army, Navy, Air National Guard, and Air Force Reserve have just completed their first deployment to a former Soviet republic. Moldova, where the medical exercise was held, thus becomes the 100th country to which US forces have been deployed this year.Working with Moldovan forces, the US military medical teams took part in rescuing, treating, and evacuating simulated victims of a hypothetical earthquake in Balti, a city of nearly 300 000 in north central Moldova. Additional joint medical training exercises are expected to be held in Moldova—which obtained its independence in 1991—in the future.The US military, and particularly its medical personnel, have been used increasingly in humanitarian and peacekeeping deployments. In the first 6 months of 1996, by comparison, US forces deployed to about 80 countries.The US Army has 33152 soldiers deployed at the moment, with about 8000 of
Vulnerable Plaque: Pathobiology and Clinical Implications
Authors: Iftikhar J. Kullo
Journal: Annals of Internal Medicine (1998)

Purpose: To review the pathobiology and clinical implications of vulnerable coronary atherosclerotic plaques and to discuss the identification of vulnerable plaques and mechanisms of plaque stabilization. Data Sources: English-language articles in the MEDLINE database that were published from 1966 to the present, identified by using the terms atherosclerotic plaque, myocardial revascularization, and plaque stabilization. Selected references cited in identified articles were reviewed. Study Selection: Experimental, clinical, and basic research studies related to coronary atherosclerotic plaques. Data Synthesis: Rupture at the site of a vulnerable atherosclerotic plaque is the most frequent cause of acute coronary syndromes. Typically, such plaque does not cause high-grade stenosis and has a large lipid core and a thin fibrous cap that is often infiltrated by inflammatory cells. Mechanical stresses contribute to plaque vulnerability, and certain triggers may cause plaque disruption directly. The most important consequence of plaque rupture is thrombosis. No method reliably identifies plaques prone to rupture. The reduction of coronary events by lipid-lowering agents despite only modest luminal changes suggests that these agents have a plaque-stabilizing effect. Surgical or percutaneous revascularization does not address the basic biology of coronary atherosclerosis and therefore may have little effect on plaque vulnerability. Conclusions: Improved understanding of the biology of atheromatous plaques has led to the concept of plaque vulnerability. Identification and stabilization of vulnerable plaques are important new directions in the treatment of coronary atherosclerosis. The relative benefits of aggressive medical therapy aimed at plaque stabilization should be compared with those of revascularization in the management of chronic coronary artery disease.
Is Interleukin-13 Critical in Maintaining Airway Hyperresposiveness in Allergen-challenged Mice?
Authors: Richard Leigh, Russ Ellis, Jennifer Wattie, Debra D. Donaldson, Mark D. Inman
Journal: American Journal of Respiratory and Critical Care Medicine (2004)

Interleukin (IL)-13 is regarded as being a central effector in the pathophysiology of airway hyperresponsiveness. We have described a mouse model in which chronic allergen exposure results in sustained airway hyperresponsiveness and aspects of airway remodeling, and here sought to demonstrate that this component of airway hyperresponsiveness is independent of biologically active IL-13. Sensitized mice were subjected to either brief or chronic periods of allergen exposure and studied 24 hours after brief or 4 weeks after chronic allergen inhalation. A soluble murine anti-IL-13 receptor fusion protein that specifically binds to and neutralizes IL-13 was given daily during the 4 days before the day of outcome measurements in both protocols. Outcome measurements included airway responses to intravenous methacholine, bronchoalveolar lavage fluid cell counts, and airway morphometry. Compared with the saline control, brief allergen challenge resulted in airway hyperresponsiveness, which was prevented by anti-IL-13 treatment. Chronic allergen challenge resulted in sustained airway hyperresponsiveness and indices of airway remodeling; IL-13 blockade failed to reverse this sustained airway hyperresponsiveness. These results confirm that IL-13 is critical for the development of airway hyperresponsiveness associated with brief allergen exposure, but is not necessary to maintain the sustained airway hyperresponsiveness associated with airway remodeling.
Controlled Evaluation of a Bandage Contact Lens and a Topical Nonsteroidal Anti-inflammatory Drug in Treating Traumatic Corneal Abrasions
Authors: Eric D. Donnenfeld, Bryan A. Selkin, Henry D. Perry, Ken Moadel, Gilbert T. Selkin, Adam J. Cohen, Lawrence T.B. Sperber
Journal: Ophthalmology (1995)
The Migratory Needle Story.
Authors: J. G. Gray
Journal: JAMA (1906)

Stillwater, Okla., Dec. 4, 1906. <h3>To the Editor:</h3> —I have read with interest the tales of foreign bodies in the flesh published inThe Journal. I had a very peculiar case of this kind. A married woman, 35 years old, stuck a needle in the middle finger of the left hand. Sixteen months later she gave birth to twins and one-half of that needle was in the corresponding finger of each twin at birth. [Several other communications have been received, but when the above letters from Drs. Morris and Gray arrived we concluded that it was time to end the discussion.—Ed.]
LETTER ON THE DEATH OF KEATS
Authors: J. R. Clemens
Journal: JAMA (1922)

<h3>To the Editor:</h3> —The following extract from the letter of Joseph Severn, dated Rome, Jan. 11, 1821, written in the sickroom of John Keats (whom Severn was nursing in his last illness) may prove of interest as showing how the Italian medical authorities viewed tuberculosis a hundred years ago: News was brought me the other day that our gentle landlady had reported to the police that my friend was dying of consumption. Now their law is — that every individual thing, even to the paper on the walls in each room the patient has been in, shall, without reserve, be destroyed by fire, the loss to be made good by his friends. This startled me not a little, for in our sitting room where I wanted to bring him, there is property worth about £150, besides all our books, etc.—invaluable. <h3>Mosquito Destruction by a Plant.</h3> —According to investigations carried out