tbuckley/cabot-search huggingface dataset:
Abstract A63: Rsf-1, a chromatin remodeling protein, interacts with shelterin protein hRap1 and induces telomere shortening
Authors: Ren-Chin Wu, Bin Guan, Yusuke Kobayashi, Tian-Li Wang, Ie-Ming Shih
Journal: Cancer Research (2013)

Abstract Background: Rsf-1, a chromatin remodeling factor that forms an ISWI chromatin-remodeling complex with SNF2H, has been implicated in the oncogenesis of a variety of human cancers, yet its functions in promoting tumor development remains unclear. In this study, we found Rsf-1 interacted with hRap1, a telomere binding protein involved in telomere length regulation. We aimed at characterizing the interaction between Rsf-1 and hRap1 and exploring the effect of Rsf-1 overexpression on telomere length in ovarian cancer cells. Methods: Protein microarray and co-immunoprecipitation were employed to screen and validate Rsf-1 interaction proteins. Deletion mutants of both Rsf-1 and hRap1 were constructed to map the binding domains responsible for their interaction. To determine whether RSF complex was enriched in the telomeric regions, we performed chromatin immunoprecipitation (ChIP) -qPCR in two ovarian cancer cell lines (OVCAR3 with high RSF-1 gene amplification and Rsf-1 inducible SKOV3) with antibodies against Rsf-1 and SNF2H. A ChIP-sequencing dataset performed with Rsf-1 and SNF2H antibodies using OVCAR3 cells was also examined for enrichment of telomeric sequences. To investigate the effect of Rsf-1 overexpression on telomere length, we evaluated by qPCR the telomere length in two Rsf-1 inducible cell lines (SKOV3 and BRK). Results: We identified hRap1, a telomere binding protein, as an interaction partner of Rsf-1 by protein microarray and co-immunoprecipitation. Protein interaction domain mapping revealed that C-terminal domain of hRAP1 and PHD domains and arginine-rich region of RSF1 were critical for this interaction. Both ChIP-qPCR and ChIP-sequencing demonstrated that Rsf-1 and SNF2H were enriched in the telomeres, suggesting that RSF complex was recruited by hRAP1 to the telomeres. Induction of RSF1 expression resulted in shortened telomere length in two different cell lines. The results suggest that RSF complex may be involved in the telomere length regulation mediated by hRAP1. Conclusions: Rsf-1 interacted with hRap1 through the PHD domain and the arginine-rich region. RSF complex bound telomeres, probably through the interaction with hRap1. Rsf-1 overexpression led to telomere length shortening, which may contribute to genomic instability in cancer cells. Citation Format: Ren-Chin Wu, Bin Guan, Yusuke Kobayashi, Tian-Li Wang, Ie-Ming Shih. Rsf-1, a chromatin remodeling protein, interacts with shelterin protein hRap1 and induces telomere shortening. [abstract]. In: Proceedings of the AACR Special Conference on Chromatin and Epigenetics in Cancer; Jun 19-22, 2013; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2013;73(13 Suppl):Abstract nr A63.
Validation de la biopsie échoguidée par échoendoscopie dans le diagnostic des adénopathies suspectes de lymphome
Authors: D Bernardini, P Grandval, H Fezoui, C Bresson, O Ramuz, C Sohn
Journal: Endoscopy (2010)

Introduction: Le diagnostic des adénopathies profondes est parfois difficile en l'absence de lésion primitive ou de ganglion accessible à une exérèse. Ce diagnostic impose habituellement un abord chirurgical coelioscopique ou non. La technique et la rentabilité de la ponction par échoendoscopie des ganglions médiastinaux et abdominaux ont été validées. Certains auteurs considèrent que la ponction à l'aiguille n'est pas contributive dans le diagnostic des lymphomes, et peut conduire à un traitement inapproprié, les limites étant l'évaluation du typage cellulaire. D'autres ont montré que la ponction à l'aiguille aboutit à un diagnostic rapide et sensible de lymphome quand la cytologie, la cytométrie de flux et l'immuno-histochimie sont utilisées en combinaison. Enfin, pour d'autres le diagnostic de lymphome par ponction est possible dans 96% des cas et bien classé dans 88% des cas malgré l'absence de cytométrie de flux, les prélèvements étant réalisés à l'aiguille 19G, sans compromettre la sécurité.
Surgical Technique and Peripheral Artery Disease
Authors: David Paul Slovut, Evan C. Lipsitz
Journal: Circulation (2012)

I nvasive treatment for patients with peripheral artery disease (PAD) has changed dramatically.Medicare claims data from 1996 to 2006 reveal an almost doubling of lower-extremity vascular procedures: The use of endovascular repair increased Ͼ3-fold, bypass surgery decreased 42%, and the amputation rate decreased by 29%. 1 For patients admitted to hospital for PAD between 1996 and 2005, the likelihood of undergoing surgery decreased from 34.5% to 26.3%. 2 Although the rate of bypass is decreasing, surgery remains an important component in the armamentarium for treating patients with advanced vascular disease.Surgical revascularization is indicated in patients with acceptable surgical risk who require a more durable repair, in those with lesions technically unsuitable for endovascular repair, and in patients who experienced failure of endovascular repair.Despite significant increases in patient morbidity, surgical bypass has become safer over the past 2 decades.Nationally, mortality after surgical bypass decreased from 7% to 4% from the 1980s to mid-1990s.From 1998 to 2003, mortality for open and combined procedures stabilized at 3% to 4%. 3 Data from the National Inpatient Survey comparing outcomes in 1998 and 2007 show significant reductions in complication rates across all categories except infection. 4Several factors may account for improved outcomes: a shift to endovascular therapy or "limited" open surgery to achieve revascularization in high-risk patients; better patient selection; more effective medication regimens consisting of a statin, an antiplatelet agent, and ␤-blocker; and improved intraoperative care and postoperative management.This review examines the risk of adverse events after vascular surgery, identifies strategies for determining and decreasing cardiac risk, and describes contemporary surgical approaches to treating patients with PAD.
Radionuclide Imaging in Extraskeletal Myxoid Chondrosarcoma
Authors: WAN-YU LIN, SHYH-JEN WANG, SHIN-HWA YEH
Journal: Clinical Nuclear Medicine (1995)

The authors present a case of extraskeletal myxoid chondrosarcoma. The MR study clearly showed the extent of the tumor and a ”rings and arcs“ pattern appeared after Gadolinium-DTPA administration in the T1 weighted images. Dynamic Tc-99m MDP and Tc-99m DTPA scintigraphy showed increased blood flow in the mass. The delayed 3-hour images demonstrated moderate Tc-99m MDP uptake, but intense Tc-99m DTPA accumulation in the tumor. Surprisingly, the Ga-67 scan failed to demonstrate the tumor.
Electrocardiographic Leads and Techniques in Mass Screening
Authors: Norman Makous
Journal: JAMA (1965)

Electrocardiographic methodology was evaluated during the multiphasic screening of 5,795 volunteers from the Philadelphia area. Approximately 20% of the electrocardiograms were abnormal or borderline. An electrocardiographic screening unit could record 100 twelve-lead tracings or more in eight hours. Each unit consisted of a technician and two volunteer assistants, one machine, and two patient electrode cables. The six limb leads were recorded four times as fast as the 12 leads and the three standard leads were recorded five times as fast. The six leads were in 96% agreement and the three leads in 93% agreement with the 12-lead classifications. The effectiveness was calculated from this agreement and from the recording speed. The six leads were found 3.8 times as effective and the three leads 4.7 times as effective as the 12 leads in mass electrocardiographic screening.
Minimization and management of wastes from biomedical research.
Authors: E H Rau, R J Alaimo, P C Ashbrook, S M Austin, N Borenstein, M R Evans, H M French, R W Gilpin, J Hughes, S J Hummel, A P Jacobsohn, C Y Lee, S Merkle, T Radzinski, R Sloane, K D Wagner, L E Weaner
Journal: Environmental Health Perspectives (2000)

Several committees were established by the National Association of Physicians for the Environment to investigate and report on various topics at the National Leadership Conference on Biomedical Research and the Environment held at the 1--2 November 1999 at the National Institutes of Health in Bethesda, Maryland. This is the report of the Committee on Minimization and Management of Wastes from Biomedical Research. Biomedical research facilities contribute a small fraction of the total amount of wastes generated in the United States, and the rate of generation appears to be decreasing. Significant reductions in generation of hazardous, radioactive, and mixed wastes have recently been reported, even at facilities with rapidly expanding research programs. Changes in the focus of research, improvements in laboratory techniques, and greater emphasis on waste minimization (volume and toxicity reduction) explain the declining trend in generation. The potential for uncontrolled releases of wastes from biomedical research facilities and adverse impacts on the general environment from these wastes appears to be low. Wastes are subject to numerous regulatory requirements and are contained and managed in a manner protective of the environment. Most biohazardous agents, chemicals, and radionuclides that find significant use in research are not likely to be persistent, bioaccumulative, or toxic if they are released. Today, the primary motivations for the ongoing efforts by facilities to improve minimization and management of wastes are regulatory compliance and avoidance of the high disposal costs and liabilities associated with generation of regulated wastes. The committee concluded that there was no evidence suggesting that the anticipated increases in biomedical research will significantly increase generation of hazardous wastes or have adverse impacts on the general environment. This conclusion assumes the positive, countervailing trends of enhanced pollution prevention efforts by facilities and reductions in waste generation resulting from improvements in research methods will continue.
Cardiothoracic surgeons do a good job
Authors: A. N J Graham
Journal: BMJ (2002)

EDITOR—Partridge provides no evidence to support his claims that cardiothoracic surgeons are less likely than thoracic surgeons to stage the extent of lung cancer objectively and assess the patients' fitness; he points out that they have “competing demands from a waiting list for coronary artery surgery.”1 He also says that relying on cardiothoracic surgeons to do lung resections may mean that some do too few to maintain competence. …
Evolution and the cardiac patient
Authors: P. HARRIS
Journal: Cardiovascular Research (1983)

Journal Article Evolution and the cardiac patient Get access PETER HARRIS PETER HARRIS From the Department of Cardiac Medicine, Cardiothoracic Institute, 2 Beaumont Street, WIN2DX Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 17, Issue 8, August 1983, Pages 437–445, https://doi.org/10.1093/cvr/17.8.437 Published: 01 August 1983
Brentuximab Vedotin in the Front-Line Treatment of Patients With CD30<sup>+</sup> Peripheral T-Cell Lymphomas: Results of a Phase I Study
Authors: Michelle A. Fanale, Steven M. Horwitz, Andres Forero-Torres, Nancy L. Bartlett, Ranjana H. Advani, Barbara Pro, Robert W. Chen, Andrew Davies, Tim Illidge, Dirk Huebner, Dana A. Kennedy, Andrei R. Shustov
Journal: Journal of Clinical Oncology (2014)

Front-line treatment of peripheral T-cell lymphomas (PTCL) involves regimens such as cyclophosphamide, doxorubicin, vincristine, prednisone (CHOP) and results in a 5-year overall survival (OS) rate of less than 50%. This phase I open-label study evaluated the safety and activity of brentuximab vedotin administered sequentially with CHOP or in combination with CHP (CHOP without vincristine) as front-line treatment in patients with CD30(+) PTCL.Patients received sequential treatment (once every 3 weeks) with brentuximab vedotin 1.8 mg/kg (two cycles) followed by CHOP (six cycles) or brentuximab vedotin 1.8 mg/kg plus CHP (BV+CHP) for six cycles (once every 3 weeks). Responders received single-agent brentuximab vedotin for eight to 10 additional cycles (for a total of 16 cycles). The primary objective was assessment of safety; secondary end points included objective response rate, complete remission (CR) rate, progression-free survival rate (PFS), and OS. There were no prespecified comparisons of the two treatment approaches.After sequential treatment, 11 (85%) of 13 patients achieved an objective response (CR rate, 62%; estimated 1-year PFS rate, 77%). Grade 3/4 adverse events occurred in eight (62%) of 13 patients. At the end of combination treatment, all patients (n = 26) achieved an objective response (CR rate, 88%; estimated 1-year PFS rate, 71%). All seven patients without anaplastic large-cell lymphoma achieved CR. Grade 3/4 adverse events (≥ 10%) in the combination-treatment group were febrile neutropenia (31%), neutropenia (23%), anemia (15%), and pulmonary embolism (12%).Brentuximab vedotin, administered sequentially with CHOP or in combination with CHP, had a manageable safety profile and exhibited substantial antitumor activity in newly diagnosed patients with CD30(+) PTCL. A randomized phase III trial is under way, comparing BV+CHP with CHOP (clinical trial No. NCT01777152).
Prioritizing Phase I Treatment Options Through Preclinical Testing on Personalized Tumorgraft
Authors: M. Pia Morelli, Emiliano Calvo, Esther Ordoñez, Michael J. Wick, Belen-Rubio Viqueira, Pedro P. Lopez-Casas, Elizabeth Bruckheimer, Antonio Calles-Blanco, David Sidransky, Manuel Hidalgo
Journal: Journal of Clinical Oncology (2011)
Atrial pacing in the prevention of atrial fibrillation after cardiac surgery: results of the second post operative pacing study (POPS-2)
Authors: R.A. Schweikert, T.A. Grady, N. Gupta, R.S. Augostini, V.I. Horiatis, S.N. French, P.M. McCarthy, M.K. Chung
Journal: Journal of the American College of Cardiology (1998)
ON THE DISPOSAL OF THE DEAD, WITH SPECIAL REFERENCE TO THE PREVALENT PRACTICE OF EMBALMING.
Authors: A. WALTER SUITER
Journal: JAMA (1895)

From remotest antiquity to the present time, topics relating to the disposal of dead bodies have been universally interesting. No subject has been more carefully considered from all points ofview, and yet it is not inconsistent with facts to state that no material advancement as to methods of preservation or final disposition has been made over those which were practiced more than twenty centuries ago. Indeed, there is a growing belief in the public mind that to return to the custom generally in vogue previous to the Christian era (cremation), would solve many perplexing problems with which sanitarians are not unfamiliar. Anciently, in Egyptian countries where the surrounding atmospheric conditions were favorable, the dead bodies of human beings were embalmed and the mummified remains indefinitely preserved. The Jewish nations entombed their dead in rock-bound receptacles called sepulchers. In Greece and Rome the practice of cremation obtained, while it appears that
ON THE FREQUENCY OF SPONTANEOUS TUMORS IN THE DOMESTIC FOWL
Authors: Margaret Schneider
Journal: The Journal of Experimental Medicine (1926)

1. Analysis of records from the Storrs Contest, the Harper Adams Trials, and a farm flock indicate an increase of tumors in the second half of the pullet year (May through October) over the first half (November through April). 2. Some evidence seems to indicate that heavy antecedent egg production is associated with a rise in tumor rate. 3. The normal annual tumor rate for fowls between the ages of 6 and 18 months is about 2 or 3 per cent. 4. The appearance of tumors in "epidemic" form probably indicates that the neoplasms found differ in etiology from those upon which the normal rate is based.
Mutilating palmoplantar keratoderma with periorificial keratotic plaques (Olmsted's syndrome)
Authors: D.J. ATHERTON, CAROLINE SUTTON, B.M. JONES
Journal: British Journal of Dermatology (1990)

We report the case of a boy, now aged 4 years, with a progressive, painful and disabling palmoplantar keratoderma, associated with well-defined hyperkeratotic plaques around the mouth and nostrils. His mother has an identical palmoplantar keratoderma. The palmoplantar keratoderma failed to improve with etretinate, but considerable benefit resulted from full-thickness excision of the skin of both palms, followed by skin grafting. We believe that he has a distinctive but extremely rare form of hereditary palmoplantar keratoderma, first described by Olmsted.
Development of ischemia/reperfusion tolerance in the rat small intestine. An epithelium-independent event.
Authors: D L Osborne, T Y Aw, G Cepinskas, P R Kvietys
Journal: Journal of Clinical Investigation (1994)

In stable organ systems, such as the heart and kidneys, an oxidant stress induces an increase in endogenous antioxidant systems resulting in an increased resistance of the tissue to a subsequent oxidant challenge. The development of this oxidant tolerance requires 1.5-6 d. The aim of the present study was to determine whether oxidant tolerance can be induced in the small intestinal mucosa, a labile system whose epithelium turns over every 2-3 d. Ischemia/reperfusion-induced epithelial barrier dysfunction of the small intestinal mucosa was monitored in Sprague-Dawley rats whose intestines had been exposed to an ischemic insult 1, 24, or 72 h previously. At 24 h, but not 1 or 72 h after the initial ischemic insult, the mucosa was more resistant to ischemia/reperfusion-induced barrier dysfunction. The antioxidant status of the mucosa was enhanced at 24 h, but not at 1 or 72 h after the initial ischemic insult. This adaptation appears to be specific for oxidants, since an initial ischemic insult imposed 24 h earlier also protected against H2O2-induced, but not acid- or ethanol-induced, barrier dysfunction. Further studies indicated that the increase in antioxidant status of the mucosa observed 24 h after the initial ischemic insult was a result of adaptational changes in the lamina propria, rather than the epithelium. In vitro studies with isolated epithelial cells also indicated that epithelial cells do not develop oxidant tolerance. We conclude that the development of oxidant tolerance in the small intestinal mucosa does not involve an active participation of the epithelial lining.
Why the GMC is right to appeal over life prolonging treatment
Authors: Raanan Gillon
Journal: BMJ (2004)

Unless a high court judgment is overturned it will skew medical care 

The General Medical Council, Britain's regulatory body for doctors, is surely right to have appealed against a high court ruling that its current guidance on withholding and withdrawing life prolonging treatment is unlawful.1 2 If not overturned the judgment is likely severely to tilt the balance of medical practice towards non-beneficial and wasteful provision of life prolonging treatment in general and of artificial nutrition and hydration in particular.

The specific case on which the high court made this ruling is uncontroversial. If the existing GMC guidance were followed Mr Burke, who took the case to the high court and who has degenerative spinocerebellar ataxia, would have been treated with artificial nutrition and hydration for rather longer than Mr Justice Munby has now ruled that he must be treated. But the judgment itself extends far beyond the particular case and can be predicted to …
Assessment of Myocardial Perfusion in Coronary Artery Disease by Magnetic Resonance
Authors: J. Schwitter, D. Nanz, S. Kneifel, K. Bertschinger, M. Büchi, P. R. Knüsel, B. Marincek, T. F. Lüscher, G. K. von Schulthess
Journal: Circulation (2001)

Background —Monitoring contrast medium wash-in kinetics in hyperemic myocardium by magnetic resonance (MR) allows for the detection of stenosed coronary arteries. In this prospective study, the quality of a multislice MR approach with respect to the detection and sizing of compromised myocardium was determined and compared with positron emission tomography (PET) and quantitative coronary angiography. Methods and Results —A total of 48 patients and 18 healthy subjects were studied by MR using a multislice hybrid echo-planar pulse sequence for monitoring the myocardial first pass kinetics of gadolinium-diethylenetriamine pentaacetic acid bismethylamide (Omniscan; 0.1 mmol/kg injected at 3 mL/s IV) during hyperemia (dipyridamole 0.56 mg/kg). Signal intensity upslope as a measure of myocardial perfusion was calculated in 32 sectors per heart from pixelwise parametric maps in the subendocardial layer and for full wall thickness. Before coronary angiography, coronary flow reserve (hyperemia induced by dipyridamole 0.56 mg/kg) was determined in corresponding sectors by 13 N-ammonia PET. Receiver-operator characteristic analysis of subendocardial upslope data revealed a sensitivity and specificity of 91% and 94%, respectively, for the detection of coronary artery disease as defined by PET (mean coronary flow reserve minus 2SD of controls) and a sensitivity and specificity of 87% and 85%, respectively, in comparison with quantitative coronary angiography (diameter stenosis ≥50%). The number of pathological sectors per patient on PET and MR studies correlated linearly (slope, 0.94; r =0.76; P &lt;0.0001). Conclusions —The presented MR approach reliably identifies patients with coronary artery stenoses and provides information on the amount of compromised myocardium, even when perfusion abnormalities are confined to the subendocardial layer. This modality may qualify for its clinical application in the management of coronary artery disease.
Linkage analysis for ATM in familial B cell chronic lymphocytic leukaemia
Authors: S Bevan, D Catovsky, A Marossy, E Matutes, S Popat, P Antonovic, A Bell, A Berrebi, EJ Gaminara, K Quabeck, I Ribeiro, FR Mauro, P Stark, H Sykes, J van Dongen, J Wimperis, S Wright, MR Yuille, RS Houlston
Journal: Leukemia (1999)
Reply to Konstantinos P. Economopoulos, Aliki Stamou, and Theodoros N. Sergentanis’ Letter to the Editor re: Luis Felipe Brandao, Riccardo Autorino, Humberto Laydner, et al. Robotic Versus Laparoscopic Adrenalectomy: A Systematic Review and Meta-analysis. Eur Urol 2014;65:1154–61
Authors: Luis Brandao, Homayoun Zargar, Riccardo Autorino
Journal: European Urology (2014)
Delayed targeting of CD39 to activated platelet GPIIb/IIIa via a single-chain antibody: breaking the link between antithrombotic potency and bleeding?
Authors: Jan David Hohmann, Xiaowei Wang, Stefanie Krajewski, Carly Selan, Carolyn A. Haller, Andreas Straub, Elliot L. Chaikof, Harshal H. Nandurkar, Christoph E. Hagemeyer, Karlheinz Peter
Journal: Blood (2013)