tbuckley/cabot-search huggingface dataset:
Abstract 2766: DNA methylation status of MLH1, p16 and SOCS1 in Puerto Rican patients with acute myeloid leukemia
Authors: Maribel Tirado-Gomez, Cristina Munoz, Paul R. Cordero, Raul Bernabe, Mercedes Lacourt, Noridza Rivera, Maribel Cotto
Journal: Cancer Research (2011)

Abstract Background: DNA methylation of gene promoters have been show to play a role in the pathogenesis of acute myeloid leukemia. We present in this study a preliminary report of the methylation status of MLH1, p16 and SOCS1 in Puertorrican patients with acute myeloid leukemia Methods: Peripheral blood was obtained from 24 patients with diagnosis of acute myeloid leukemia before their induction treatments. DNA was isolated and further modified with sodium bisulfite. Methylation-specific polymerase (MSP) chain reaction was performed to detect aberrant promoter methylation of MLH1, p16 and SOCS1. Results: A total of 24 samples were analyzed. The mean age was 49 years (range 22-71) with 17/24 patients younger than 60 years (71%). Most of the patients were female (13/24, 54%). Six patients had diploid cytogenetics (25%), 6 had low risk cytogenetics (25%) and 12 had high risk cytogenetics (50%). Fifteen patients were de Novo AML (63%). Nine patients (38%) presented with WBC counts higher than 10 × 109/L. Thirteen patients (54%) achieved a complete response at the end of the induction therapy. Methylation of MLH1 was detected in 3/24 patients (13%). Methylation of p16 was detected in 5/24 patients (21%). Methylation of SOCS1 was detected in 2/24 patients (8%). Conclusions: A tendency toward an association of methylated p16 with treatment resistance was noted. Also, methylation of SOCS1 was more common among relapsed AML patients. No correlation between methylated MLH1 gene and clinicopathological characteristics was noted. Collection of more patient's samples and correlation with clinical data and patient's ultimate outcome is ongoing. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2766. doi:10.1158/1538-7445.AM2011-2766
Successful treatment of Barrett’s esophagus with radiofrequency ablation in a patient with severe reflux esophagitis following fundoplication
Authors: Stephen Oh, John Gilliam, Jason Conway
Journal: Endoscopy (2014)

We read with interest the article by van Vilsteren et al. titled “Predictive factors for initial treatment response after circumferential radiofrequency ablation for Barrett’s esophagus with early neoplasia: a prospective multicenter study,” published in a recent issue of Endoscopy [1]. The authors found the presence of reflux esophagitis to be the strongest predictor of a poor initial response after circumferential radiofrequency ablation (RFA) and suggested either optimizing medical therapy or fundoplication prior to RFA in order to increase the success rates of RFA [1].
A fully human anti-hepcidin antibody modulates iron metabolism in both mice and nonhuman primates
Authors: Keegan S. Cooke, Beth Hinkle, Hossein Salimi-Moosavi, Ian Foltz, Chadwick King, Palaniswami Rathanaswami, Aaron Winters, Shirley Steavenson, C. Glenn Begley, Graham Molineux, Barbra J. Sasu
Journal: Blood (2013)
Maternal Connective Tissue Disease and Congenital Heart Block
Authors: Stephen E. Litsey, Jacqueline A. Noonan, William N. O'Connor, Carol M. Cottrill, Bonnie Mitchell
Journal: New England Journal of Medicine (1985)

A RECENT report by Scott et al. described antibodies to a soluble tissue ribonucleoprotein antigen called Ro(SS-A) in the serum of 34 of 41 mothers who had given birth to infants with congenital complete heart block.1 Less than half the mothers had clinical evidence of connective tissue disease. The same antibody was found in 7 of 8 serum samples collected from affected infants under three months of age but in none of 13 samples obtained from older infants. The authors suggested that maternal antibody to Ro(SS-A) might serve as a marker to identify women at risk of having an infant . . .
Social deprivation and diabetic patients
Authors: J Christensen, J M Moller
Journal: BMJ (1994)

EDITOR, - William F Kelly and colleagues link social deprivation and illness in diabetic patients.1 It would have been informative to know whether the patients living in the deprived wards were not using insulin simply because they had non-insulin dependent diabetes, which would be in agreement with their older age. Was the prevalence of insulin dependent diabetes comparable in the wards? In an earlier Danish study that linked diabetic ketoacidosis to social deprivation, patients from the lowest social classes had …
How Important Is DNA Replication for Mutagenesis?
Authors: Gavin A. Huttley, Ingrid B. Jakobsen, Susan R. Wilson, Simon Easteal
Journal: Molecular Biology and Evolution (2000)

Rates of mutation and substitution in mammals are generally greater in the germ lines of males. This is usually explained as resulting from the larger number of germ cell divisions during spermatogenesis compared with oogenesis, with the assumption made that mutations occur primarily during DNA replication. However, the rate of cell division is not the only difference between male and female germ lines, and mechanisms are known that can give rise to mutations independently of DNA replication. We investigate the possibility that there are other causes of male-biased mutation. First, we show that patterns of variation at approximately 5,200 short tandem repeat (STR) loci indicate a higher mutation rate in males. We estimate a ratio of male-to-female mutation rates of approximately 1.9. This is significantly greater than 1 and supports a greater rate of mutation in males, affecting the evolution of these loci. Second, we show that there are chromosome-specific patterns of nucleotide and dinucleotide composition in mammals that have been shaped by mutation at CpG dinucleotides. Comparable patterns occur in birds. In mammals, male germ lines are more methylated than female germ lines, and these patterns indicate that differential methylation has played a role in male-biased vertebrate evolution. However, estimates of male mutation bias obtained from both classes of mutation are substantially lower than estimates of cell division bias from anatomical data. This discrepancy, along with published data indicating slipped-strand mispairing arising at STR loci in nonreplicating DNA, suggests that a substantial percentage of mutation may occur in nonreplicating DNA.
Matrix Metalloproteinase-19 Is a Key Regulator of Lung Fibrosis in Mice and Humans
Authors: Guoying Yu, Elisabetha Kovkarova-Naumovski, Paul Jara, Anil Parwani, Daniel Kass, Victor Ruiz, Carlos Lopez-Otín, Ivan O. Rosas, Kevin F. Gibson, Sandra Cabrera, Remedios Ramírez, Samuel A. Yousem, Thomas J. Richards, Lara J. Chensny, Moisés Selman, Naftali Kaminski, Annie Pardo
Journal: American Journal of Respiratory and Critical Care Medicine (2012)

Rationale: Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by epithelial phenotypic changes and fibroblast activation. Based on the temporal heterogeneity of IPF, we hypothesized that hyperplastic alveolar epithelial cells regulate the fibrotic response.Objectives: To identify novel mediators of fibrosis comparing the transcriptional signature of hyperplastic epithelial cells and conserved epithelial cells in the same lung.Methods: Laser capture microscope and microarrays analysis were used to identify differentially expressed genes in IPF lungs. Bleomycin-induced lung fibrosis was evaluated in Mmp19-deficient and wild-type (WT) mice. The role of matrix metalloproteinase (MMP)-19 was additionally studied by transfecting the human MMP19 in alveolar epithelial cells.Measurements and Main Results: Laser capture microscope followed by microarray analysis revealed a novel mediator, MMP-19, in hyperplastic epithelial cells adjacent to fibrotic regions. Mmp19−/− mice showed a significantly increased lung fibrotic response to bleomycin compared with WT mice. A549 epithelial cells transfected with human MMP19 stimulated wound healing and cell migration, whereas silencing MMP19 had the opposite effect. Gene expression microarray of transfected A549 cells showed that PTGS2 (prostaglandin–endoperoxide synthase 2) was one of the highly induced genes. PTGS2 was overexpressed in IPF lungs and colocalized with MMP-19 in hyperplastic epithelial cells. In WT mice, PTGS2 was significantly increased in bronchoalveolar lavage and lung tissues after bleomycin-induced fibrosis, but not in Mmp19−/− mice. Inhibition of Mmp-19 by siRNA resulted in inhibition of Ptgs2 at mRNA and protein levels.Conclusions: Up-regulation of MMP19 induced by lung injury may play a protective role in the development of fibrosis through the induction of PTGS2.
Periodic fever, aphthous stomatitis, pharyngitis, cervical adenitis syndrome is linked to dysregulated monocyte IL-1β production
Authors: Laeticia Kolly, Nathalie Busso, Annette von Scheven-Gete, Nathaliane Bagnoud, Isabelle Moix, Dirk Holzinger, Gregoire Simon, Annette Ives, Greta Guarda, Alexander So, Michael A. Morris, Michaël Hofer
Journal: Journal of Allergy and Clinical Immunology (2012)
[347] THYROID HORMONE INDUCES GROWTH ARREST AND ACTIVATION OF HEPATIC STELLATE CELLS OVEREXPRESSING THYROID HORMONE RECEPTOR ALPHA1, BUT NOT THYROID HORMONE RECEPTOR BETA 1
Authors: I. Zvibe, A. Phillips, D. Alias, S. Abramovitch, S. Vigoodman, S. Ringelstein, Z. Kraiem, Z. Halpern, R. Oren
Journal: Journal of Hepatology (2007)
733-4 Induction of Inducible Nitric Oxide Synthase (iNOS) mRNA and Protein in the Human Cardiac Allograft is Associated with Increased Myocardial cGMP Levels and Echocardiographic LV Dysfunction
Authors: Neil P. Lewis, Philip S. Tsao, Peter R. Rickenbacher, Guy A. Haywood, Heiko von der Leyen, Sharon A. Hunt, Margaret E. Billingham, Hannah A. Valantine, Michael B. Fowler
Journal: Journal of the American College of Cardiology (1995)
Breast-milk Jaundice and Oral Contraceptives
Authors: Y. K. Wong, B. S. B. Wood
Journal: BMJ (1971)

Among breast-fed infants in the normal lying-in wards of a maternity hospital a significantly higher incidence of “idiopathic” jaundice was found in infants of mothers who had been receiving the contraceptive pill before the present pregnancy than in the infants whose mothers never had the pill. The pill became widely used in society at about the same time as breast-milk jaundice was first reported.
Exquisite food allergy without eating
Authors: S. L. Bahna
Journal: Allergy (1994)

The past few decades have witnessed an increasing understanding of the role of food allergy in human disease. Nevertheless, controversies still exist in several aspects of this area. One such controversy concerns the body systems that can be the target of food hypersensitivity reactions. Another is the role of noningestant exposure to food in a nonoccupational setting. Both of these aspects are vividly demonstrated in the case presented here.
Variceal pressure is a strong predictor of variceal haemorrhage in patients with cirrhosis as well as in patients with non-cirrhotic portal hypertension
Authors: E A El Atti, F Nevens, K Bogaerts, G Verbeke, J Fevery
Journal: Gut (1999)

Variceal pressure is a strong predictor for a first variceal bleed in patients with cirrhosis.To evaluate whether variceal pressure is also a determinant of the risk of a first variceal bleed in patients with non-cirrhotic portal hypertension.Variceal pressure was measured non-invasively in 25 patients with non-cirrhotic portal hypertension and large varices while receiving a stable therapeutic regimen. Factors predictive of bleeding were compared with those observed in 87 cirrhotics.The one year incidence of variceal bleeding was 32% (n=28) for the cirrhotic and 20% (n=5) for the non-cirrhotic patients. There was no difference in factors predicting the risk of bleeding between the groups, except for variceal pressure. For the same level of variceal pressure, the risk of variceal bleeding was lower in patients with non-cirrhotic portal hypertension. Multiple logistic regression analysis revealed the following variables as having a significant predictive power: variceal pressure (p=0.0001), red spots (p=0.004), and the time interval between the first observation of the varices and the moment of variceal pressure measurement (p=0. 0046). For the non-cirrhotics the risk of bleeding increased with higher Child-Pugh score (p=0.0024); this was not the case for the cirrhotic patients (p=0.9521).Variceal pressure is a major predictor of variceal bleeding in patients with cirrhosis as well as in patients with non-cirrhotic portal hypertension. The risk of bleeding in non-cirrhotics is less than in cirrhotics for the same level of variceal pressure. In patients with non-cirrhotic portal hypertension the risk of variceal bleeding increases more with advancing disease.
High-Frequency Oscillatory Ventilation on Shaky Ground
Authors: Atul Malhotra, Jeffrey M. Drazen
Journal: New England Journal of Medicine (2013)

We thought it was impossible. Physiological principles maintained that ventilation at tidal volumes less than the anatomical deadspace should be ineffective (i.e., inspired air not reaching the alveolae). Data from a 1980 study dispelled that myth, showing unequivocally that ventilation with tidal volumes as small as 20 to 30 ml in dogs, a mere fraction of the anatomical deadspace, could maintain adequate ventilation.1 These unexplained observations sparked transport and mixing theories predicting that CO2 removal should vary in direct proportion to breathing frequency (although the relationship with tidal volume is more complex2,3), and these predictions were later . . .
Lack of effect of lovastatin therapy on the parameters of whole-body cholesterol metabolism.
Authors: I J Goldberg, S Holleran, R Ramakrishnan, M Adams, R H Palmer, R B Dell, D S Goodman
Journal: Journal of Clinical Investigation (1990)

The effects of lovastatin therapy on the parameters of body cholesterol metabolism were explored in nine hypercholesterolemic patients. Long-term cholesterol turnover studies were performed before therapy, and were repeated after 15 mo of lovastatin therapy (40 mg/d) while continuing on therapy. The major question addressed was whether a reduction in plasma cholesterol level with lovastatin would be associated with a reduction in the whole-body production rate of cholesterol or with the sizes of exchangeable body cholesterol pools as determined by the three-pool model of cholesterol turnover. The mean plasma cholesterol level decreased 19.4% (from 294 to 237 mg/dl), and low-density lipoprotein cholesterol decreased 23.8% (from 210 to 159 mg/dl) with lovastatin therapy. Changes in high-density lipoprotein cholesterol level were not significant. The cholesterol production rate did not change significantly with therapy (1.09 +/- 0.10 [mean +/- S.D.] vs. 1.17 +/- 0.09 g/d). By comparison, colestipol and niacin treatment in three other subjects more than doubled the cholesterol production rate (1.14 +/- 0.28 vs. 2.42 +/- 0.34 g/d). Thus, hydroxymethylglutaryl-coenzyme A (HMG-CoA) reductase inhibition by lovastatin at the therapeutic dose used here did not change the steady-state rate of whole-body cholesterol synthesis. Despite the changes in plasma cholesterol levels, no significant changes were seen in the values of M1, of M3 or of Mtot, the sizes of the pools of rapidly, of slowly, and of total body exchangeable cholesterol. Conclusion: lovastatin therapy to lower plasma cholesterol does not lead to corresponding reductions in body cholesterol pools or to a reduction in the rate of whole-body cholesterol synthesis. In the new steady state that exists during long-term lovastatin therapy, along with increased expression of the genes for HMG-CoA reductase and the LDL receptor, the body compensates for the effects of the drug so that cholesterol production rate and tissue pool sizes are not changed from pretreatment values.
Resuscitation and Artificial Hypothermia
Authors: John B. Stetson
Journal: JAMA (1963)

The author's preface suggested an interesting book which covers the areas of cardiac massage, intra-arterial transfusion, defibrillation of the heart, restoration of breathing, maintenance of the viability of the brain, role of hypothermia, clinical application of experimental findings, use of this material by anesthesiologists, and a comparison of the author's results with reports in the literature. To the reviewer this book has three intermingled phases. They are historical development, a survey of the then current (1961) literature, and a polemic for the author's ideas, especially with regard to intra-arterial transfusions. The historical development is interesting and yields to the American reader many European source references. Like all surveys of the current literature, this book was out of date the day it was written. The reader will also find many references in the English language missing, references that most readers would consider important (this despite a 39 page bibliography). The translator
Trends in Milk, Egg, and Peanut IgE Levels Over Time in an Observational Study of Infants with Food Allergy (COFAR2): One Year Follow Up
Authors: R.A. Wood, S. Sicherer, D. Stablein, S. Jones, A.W. Burks, A. Liu, D. Fleischer, D.Y.M. Leung, R. Lindblad, H. Sampson
Journal: Journal of Allergy and Clinical Immunology (2009)
Abstract 943: Characterization of RET and integrin signaling in thyroid tumors
Authors: Eric Lian, Jessica Cockburn, Sonal Varma, Katayoon Kasaian, Steven J.M. Jones, Victor Tron, Sam Wiseman, Lois Mulligan
Journal: Cancer Research (2012)

Abstract The REarranged in Transfection (RET) tyrosine kinase is important during development of neural crest derived tissues such as the enteric and sympathetic nervous systems, but is also activated in multiple human tumour types. Activating mutations of RET play an important role in thyroid cancers including both papillary thyroid carcinoma (PTC) and medullary thyroid carcinoma (MTC). More broadly, recent studies have also shown that RET may be active in other tumour types such as pancreatic and breast cancers, and that the overexpression and activation of RET may be linked to more aggressive disease. In order to explore this link, we have investigated the expression of RET in a large cohort of over 400 thyroid tumour samples of multiple histologies, ranging from benign lesions through to aggressive, metastatic disease by immunhistochemistry using a thyroid tissue microarray. Our data suggest that RET expression may correlate with more aggressive phenotype across a broader spectrum of tumours than previously predicted. We are currently investigating this association further; first by examining the expression of two major RET protein isoforms, RET9 and RET51, in this panel of tumours, and second, by investigating the coexpression of RET with another group of proteins associated with tumour progression, the integrin family. In preliminary studies, we have evaluated RET coexpression with two integrin subunits, ITGB1 and ITGB3. Our data suggest correlation between tumour aggressiveness and coexpression of RET with these integrin subunits. Together, our data provides insight on the role of RET in increasing aggressiveness over a range of tumours. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 943. doi:1538-7445.AM2012-943
Lipid Deposits (Islands) in Gastric Mucosa
Authors: G. Clémençon, F. Gloor
Journal: Endoscopy (1974)

The knowledge of the existence of lipid islands in gastric mucosa is of some diagnostic value, since the lesions may be mistaken for superficial gastric cancer gastroscopically as well as histologically. Lipid islands seem to occur more frequently than hitherto assumed. 3 cases with tiny lipid islands in gastric mucosa are described.
Blackburn Schoolgirl Outbreak
Authors: J. Ardley, P. Grime
Journal: BMJ (1967)

BRITIS 363 on 50 tablets per Week.Unlike the two cases reported, however, she had underlying rheumatic carditis.I attempted substitution with dummy tablets, but these were recognized as such immediately, and rejected.It would appear that to an addict only dextromoramide tastes like dextromoramide.Similarly, as with the five cases reported, she develops pain (posteriorly in the base of her right lung) if administration is delayed, and wakes approximately two-hourly for further dosage.She is now a registered addict taking 190 5-mg.tablets per week.-Iam, etc., Yardley, B. A. JuBY. Birmingham. BPhenothiazines in Early Labour SIR,-We read with interest your leading article on sedation in early labour (26 November, p. 1282), in particular the use of perphenazine (Fentazin).We agree that the drug is safe, but would like to report two patients we have recently seen who were treated with perphenazine for hyperemesis gravidarum, and who developed dyskinetic side-effects which in each case led to an initial diagnosis of tetany.A 24-year-old lady on admission was seven and a half weeks pregnant and had had hyperemesis for four weeks.This had been treated with a variety of tablets with no success.Two
Book: Essentials of Telemedicine and Telecare
Authors: E. Coiera
Journal: BMJ (2002)

A C Norris John Wiley and Sons, £27.50, pp 177 ISBN 0 471 53151 0 Rating: ![Graphic][1]</img>![Graphic][2]</img>![Graphic][3]</img> Telemedicine is the use of information and communication technology to transfer information in the support of health care, and has for much of its history been curiosity or conviction driven. While the early roots of telemedicine arose out of the need to support remote communities, much of the modern endeavour, like telesurgery, is almost all about extending the boundaries of the possible. It is only in the last few years that … [1]: /embed/inline-graphic-1.gif [2]: /embed/inline-graphic-2.gif [3]: /embed/inline-graphic-3.gif