tbuckley/cabot-search huggingface dataset:
Abstract A34: A mechanism for acquired resistance to estrogen deprivation in breast cancer cells
Authors: Helena Aguilar, Nuria Bonifaci, Xavier Sole, Jordi Serra Musach, Victor Moreno, Conxi Lazaro, Ander Urruticoechea, Miguel Angel Pujana
Journal: Clinical Cancer Research (2010)

Abstract Endocrine therapies that target the proliferative effect of 17β-estradiol (17βE2) through estrogen receptor α (ERα) are the most effective form of systemic treatment of ERα-positive breast cancer. Nevertheless, through mechanisms that are not fully understood, most tumors that initially respond to therapy will recur. The long-term estrogen deprived (LTED) MCF7 cell model has been proposed to recapitulate acquired resistance to aromatase inhibition (AI) in postmenopausal women. To comprehensively elucidate this resistance, genomic, transcriptomic and molecular analyses were integrated into the time course of MCF7-LTED adaptation. Extensive and dynamic genomic changes were observed, including amplification of the ESR1 locus linked to an increase in ERα and its non-genomic role. Dynamic transcriptomic profiles were also observed that significantly correlated with genomic changes and that were influenced by transcription factors known to be involved in acquired resistance (IRF-1) and cell proliferation (E2F1) but not by canonical ERα genomic function. Evaluation of MCF7-LTED profiles association with pathological and clinical data shown consistent correlations with ERα-negative tumor status, letrozole effects and recurrence after tamoxifen treatment. This study describes the coordinated genomic, transcriptomic and molecular changes that mediate acquired resistance to endocrine therapies, suggesting a key role for the non-genomic function of ERα. Citation Information: Clin Cancer Res 2010;16(7 Suppl):A34
Common genetic variation of the cholesteryl ester transfer protein gene strongly predicts future cardiovascular death in patients with coronary artery disease
Authors: Stefan Blankenberg, Hans J Rupprecht, Christoph Bickel, Xian-Cheng Jiang, Odette Poirier, Karl J Lackner, Jürgen Meyer, François Cambien, Laurence Tiret
Journal: Journal of the American College of Cardiology (2003)
Brokering a deal to replace Kyoto will take years, EU ministers say
Authors: J. Vidal
Journal: BMJ (2011)

Hopes of a breakthrough to unblock the stuttering United Nations talks on climate change remained slim this week as ministers from 190 countries flew to Durban, South Africa.

With Canada, Russia, and Japan refusing to sign up to a second period of the Kyoto protocol, and the United States stating it would not be legally bound to any treaty unless poor countries were too, it was left to the European Union to try to broker a compromise. The Kyoto protocol, which legally binds all rich countries except the US to cuts, runs out in 2012.

The EU’s Durban “roadmap” …
Risk Factors for Infusion-related Phlebitis with Small Peripheral Venous Catheters
Authors: Dennis G. Maki, Marilyn Ringer
Journal: Annals of Internal Medicine (1991)

Objective: To identify risk factors for infusion-related phlebitis with peripheral intravenous catheters. Design: A randomized trial of two catheter materials, with consideration of 21 potential risk factors. Setting: A university hospital. Patients: Hospitalized adults without granulocytopenia who received a peripheral intravenous catheter. Interventions: House officers or ward nurses inserted the catheters, and each insertion was randomized to a catheter made of tetrafluoroethylene-hexafluoropropylene (FEP-Teflon) or a novel polyetherurethane without teachable additives (PEU-Vialon). Measurements: Research nurses scored insertion sites each day for inflammation and cultured catheters at removal. Results: The Kaplan-Meier risk for phlebitis exceeded 50% by day 4 after catheterization. Intravenous antibiotics (relative risk, 2.00), female sex (relative risk, 1.88), prolonged (> 48 hours) catheterization (relative risk, 1.79), and catheter material (PEU-Vialon: FEP-Teflon) (relative risk, 0. 73) strongly predicted phlebitis in a Cox proportional hazards model (each, P < 0.003). The best-fit model for severe phlebitis identified the same predictors plus catheter-related infection (relative risk, 6.19), phlebitis with a previous catheter (relative risk, 1.54), and anatomic site (hand:forearm, relative risk, 0.71; wrist:forearm, relative risk, 0.60). The low incidence of local catheter-related infection was comparable with the two catheter materials (5.4% [95% CI, 3.8% to 7.6%] and 6.9% [CI, 4.9% to 9.6%]); none of the 1054 catheters prospectively studied caused bacteremia. Conclusions: Multiple factors, including the Infusate and the duration of cannulation, contribute to the development of infusion-related phlebitis. The use of peripheral intravenous catheters made of PEU-Vialon appears to pose the same risk for catheter-related infection as the use of catheters made of FEP-Teflon, and PEU-Vialon can permit longer cannulation with less risk for phlebitis. The risk for catheter-related bacteremia with FEP-Teflon and PEU-Vialon catheters is sufficiently low that it no longer seems justifiable to recommend the use of small steel needles for most peripheral intravenous therapy. Annals of Internal Medicine. 1991;114:845-854.
Protein Kinase B Controls Transcriptional Programs that Direct Cytotoxic T Cell Fate but Is Dispensable for T Cell Metabolism
Authors: Andrew N. Macintyre, David Finlay, Gavin Preston, Linda V. Sinclair, Caryll M. Waugh, Peter Tamas, Carmen Feijoo, Klaus Okkenhaug, Doreen A. Cantrell
Journal: Immunity (2011)
Gemtuzumab ozogamicin as postconsolidation therapy does not prevent relapse in children with AML: results from NOPHO-AML 2004
Authors: Henrik Hasle, Jonas Abrahamsson, Erik Forestier, Shau-Yin Ha, Jesper Heldrup, Kirsi Jahnukainen, Ólafur Gísli Jónsson, Birgitte Lausen, Josefine Palle, Bernward Zeller
Journal: Blood (2012)
Different Costimulation Signals Used by CD4+ and CD8+ Cells That Independently Initiate Rejection of Allogenic Hepatocytes in Mice
Authors: Donghong Gao, Jiashun Li, Charles G. Orosz, Ginny L. Bumgardner
Journal: Hepatology (2000)

The current study evaluated the role of CD40/CD40 ligand (CD40L) and CD28/B7 costimulation signals during alloimmune responses independently mediated by CD4 + or CD8 + T cells. Allogeneic hepatocytes were transplanted into CD8 or CD4 knock out (KO) mice under cover of costimulatory blockade. Rejection of FVB/N (H–2 q ) hepatocytes occurred by day 10 posttransplant in untreated CD8 or CD4 KO (H–2 b ) mice. Treatment of CD8 or CD4 KO mice with anti–CD40L monoclonal antibody (mAb; MR1) resulted in significant prolongation of hepatocyte survival indicating that CD40/CD40L interactions were critical in both CD4 + and CD8 + T–cell initiated hepatocyte rejection. Anti–CD40L mAb also prolonged hepatocyte survival in B–cell KO (H–2 b ) mice, indicating that the efficacy of CD40/CD40L blockade in preventing hepatocyte rejection was B–cell (and antibody) independent. In contrast, treatment with CTLA4 fusion protein (CTLA4Ig), prolonged hepatocyte survival in CD8 KO but not CD4 KO mice, showing that CD28/B7 interactions were important in CD4 + but not CD8 + T–cell initiated hepatocyte rejection. Under selected circumstances, such as in CD40 KO mice, both CD4 + and CD8 + T cells mediate hepatocyte rejection in the absence of CD40/CD40L costimulation and without a significant contribution from CD28/B7 costimulation signals. These results highlight the disparate roles of CD40/CD40L and CD28/B7 costimulation signals in CD4 + versus CD8 + T–cell mediated immune responses to allogeneic hepatocytes. The CD4 + T–cell independent, CD40L–sensitive, CD28/B7–independent pathway of CD8 + T–cell activation in response to transplantation antigens is novel.
Are Active Video Games Useful in Increasing Physical Activity and Addressing Obesity in Children?—Reply
Authors: Stephen R. Smallwood, Michael M. Morris, Stephen J. Fallows, John P. Buckley
Journal: JAMA Pediatrics (2013)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Pediatrics HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Pediatrics journal
Scintigraphic and CT Findings of Tietze??s Syndrome: Report of a Case and Review of the Literature
Authors: NORINARI HONDA, KIKUO MACHIDA, TOSHIO MAMIYA, TAKU TAKAHASHI, TERUO TAKISHIMA, NORIKO HASEGAWA, TSUYOSHI KAMANO, MASANORI HASHIMOTO, KEN OHNO, SHINJI ITOYAMA, NOBUYUKI TSUZUKI
Journal: Clinical Nuclear Medicine (1989)

A case of Tietze's syndrome is reported. A 43-year-old woman, who had experienced right anterior chest pain and tender swelling of the right first costosternal junction for seven months, showed increased accumulation of the right first, the right fourth, and the left first costochondral junction on bone imaging. Ga-67 imaging showed increased accumulation at the right first costosternal junction. CT showed sclerosis of the sternal manubrium, partial calcification of costal cartilage, and soft tissue swelling. Biopsy of the right first costal cartilage showed chronic inflammation with fibrosis and ossification. Increased uptake of bone gallium imaging is consistent with Tietze's syndrome.
Detection of a protein-acetaldehyde adduct in the liver of rats fed alcohol chronically.
Authors: R C Lin, R S Smith, L Lumeng
Journal: Journal of Clinical Investigation (1988)

We report here the formation in vivo of a protein-acetaldehyde adduct (protein-AA) in liver when rats were fed alcohol chronically. This chemically modified protein was demonstrated by electroimmunotransblot technique and with rabbit polyclonal antibodies that recognize acetaldehyde adduct as an epitope (i.e., both anti-hemocyanin-AA IgG and anti-myoglobin-AA IgG). It has a molecular weight of 37,000. It can be detected in the liver of rats fed the alcohol-containing American Institute of Nutrition 1976 liquid diet for only 1 wk. Since the protein profiles of soluble hepatic proteins from alcohol-fed and control rats were identical on SDS-PAGE, the peroxidase-positive band demonstrated by electroimmunotransblot was most likely not a new protein synthesized de novo. Borohydride reduction was not necessary to stabilize this protein-AA. Intraperitoneal injections of ethanol (2 g/kg body wt) at 8-h intervals to rats over a 24-h period did not produce any detectable protein-AA in the liver. Incubation of the liver homogenate from a control liver with acetaldehyde without sodium cyanoborohydride for 4 h also failed to generate any protein-AA. Therefore, the formation of the 37-kD protein-AA in vivo reported here is dependent on chronic alcohol consumption.
Internalized Antigens Must Be Removed to Prepare Hypoimmunogenic Mesenchymal Stem Cells for Cell and Gene Therapy
Authors: Jeffrey L. Spees, Carl A. Gregory, Harpreet Singh, H.Alan Tucker, Alexandra Peister, Patrick J. Lynch, Shu-Ching Hsu, Jason Smith, Darwin J. Prockop
Journal: Molecular Therapy (2004)

Adult stem cells from human bone marrow stroma, referred to as mesenchymal stem cells or marrow stromal cells (hMSCs), are attractive candidates for clinical use. The optimal conditions for hMSC expansion require medium supplemented with fetal calf serum (FCS). Some forms of cell therapy will involve multiple doses, raising a concern over immunological reactions caused by medium-derived FCS proteins. By a sensitive fluorescence-based assay we determined that 7 to 30 mg of FCS proteins are associated with a standard preparation of 100 million hMSCs, a dosage that probably will be needed for clinical therapies. Here we present ex vivo growth conditions for hMSCs that reduce the FCS proteins to less than 100 ng per 100 million hMSCs, approximately a 100,000-fold reduction. The cells maintain their proliferative capacity and sustain their ability for multilineage differentiation. Experiments in rats demonstrate that rat MSCs grown in 20% FCS induce a substantial humoral response after repeated administrations, whereas cells grown under the conditions described in this study reduce the immunogenicity in terms of IgG response over 1000-fold to barely detectable levels. Our results have the potential to dramatically improve cellular and genetic therapies using hMSCs and perhaps other cells.
Fate and Complex Pathogenic Effects of Dioxins and Polychlorinated Biphenyls in Obese Subjects before and after Drastic Weight Loss
Authors: Min-Ji Kim, Philippe Marchand, Corneliu Henegar, Jean-Philippe Antignac, Rohia Alili, Christine Poitou, Jean-Luc Bouillot, Arnaud Basdevant, Bruno Le Bizec, Robert Barouki, Karine Clément
Journal: Environmental Health Perspectives (2010)

In humans, persistent organic pollutants (POPs) are stored primarily in adipose tissue. Their total body burden and their contribution to obesity-associated diseases remain unclear.We characterized POP total body burden and their redistribution in obese individuals before and after drastic weight loss and compared these values with a variety of molecular, biological, and clinical parameters.Seventy-one obese subjects were enrolled and underwent bariatric surgery. Blood and adipose tissue samples were obtained at different times from these individuals as well as from 18 lean women.POP content (17 dioxins/furans and 18 polychlorinated biphenyl congeners) in different adipose tissue territories was similar, allowing us to assess total POP body burden from a single biopsy. Total POP body burden was 2 to 3 times higher in obese than in lean individuals. We also found increased expression of some POP target genes in obese adipose tissue. Drastic weight loss led to increased serum POPs and, within 6-12 months, to a significant 15% decrease in total polychlorinated biphenyl body burden. Importantly, serum POP levels were positively correlated with liver toxicity markers and lipid parameters, independently of age and body mass index.POP content in adipose tissue and serum correlate with biological markers of obesity-related dysfunctions. Drastic weight loss leads to a redistribution of POPs and to a moderate decrease of their total body burden.
Smoking Makes Knaves Little. Kills Them, Too.
Authors: Allen J. Wilcox
Journal: JAMA (1994)

To the Editor.  —In 1547, Thomas Seymour gave the following advice to his pregnant wife, Queen Catherine (widow of Henry VIII): I do desire your Highness to keep the little knave so lean and gaunt with your good diet and walking, that he may be so small that he may creep out of a mousehole.1 If Thomas Seymour were alive today, perhaps he would also recommend that his wife smoke. Cigarette labels carry the message that [s]moking by pregnant women may result in fetal injury, premature birth and low birth weight. Health professionals know low birth weight is associated with perinatal death, but a layperson can too easily misinterpret small fetal size as an advantage.  The current warning to pregnant women is completely obsolete. It dates from an era when the effects of cigarette smoking during pregnancy were less clear. For a while, the picture was muddled by the paradoxical
Drug-Eluting Stenting Followed by Cilostazol Treatment Reduces Late Restenosis in Patients With Diabetes Mellitus
Authors: Seung-Whan Lee, Seong-Wook Park, Young-Hak Kim, Sung-Cheol Yun, Duk-Woo Park, Cheol Whan Lee, Myeong-Ki Hong, Hyun-Sook Kim, Jae-Ki Ko, Jae-Hyeong Park, Jae-Hwan Lee, Si Wan Choi, In-Whan Seong, Yoon Haeng Cho, Nae-Hee Lee, June Hong Kim, Kook-Jin Chun, Seung-Jung Park
Journal: Journal of the American College of Cardiology (2008)
Bleeding Due to Increased Intravascular Blood Coagulation
Authors: F. Rodríguez-Erdmann
Journal: New England Journal of Medicine (1965)

THROMBOSIS and hemorrhage are generally considered as diametrically opposed conditions. There is, however, a group of disorders in which generalized thrombosis and hemorrhagic tendency are intimately related. They are characterized by a hemorrhagic diathesis of acute onset and a clinical picture dominated by severe circulatory collapse. The pathogenesis of this syndrome is poorly understood, and only recently has it become clear that the hemorrhagic tendency is a consequence of the acute activation of the clotting mechanism, which results in the intravascular consumption of plasma clotting factors. In other words, the circulating plasma is transformed into circulating serum.This group . . .
Reentrant and nonreentrant mechanisms contribute to arrhythmogenesis during early myocardial ischemia: results using three-dimensional mapping.
Authors: S M Pogwizd, P B Corr
Journal: Circulation Research (1987)

The present study assessed the mechanisms responsible for the initiation and maintenance of premature ventricular complexes (PVCs) and ventricular tachycardia (VT) during early ischemia using a unique computerized mapping system capable of recording simultaneously from 232 individual intramural sites. In the chloralose-anesthetized cat, during normal sinus rhythm prior to ischemia, ventricular activation was rapid with a total activation time of 25 +/- 2 msec. Five minutes after occlusion of the left anterior descending (LAD) coronary artery, activation was delayed during sinus rhythm (64 +/- 6 msec) (p less than 0.001 vs. control) and was characterized by slow conduction in the same plane and block in both the same plane and in the endocardial-to-epicardial direction. In 76% of cases (16 of 21), initiation of single PVCs and the first beat of VT occurred through intramural reentry. In all but one case, initiation occurred in the subendocardium, adjacent to the site of delayed subendocardial and midmyocardial activation of the preceding sinus beat. The activation time of the sinus beat preceding the PVC or VT was significantly prolonged (149 +/- 7 msec, p less than 0.001 vs. sinus beats during ischemia not followed by a PVC or VT) with most of the delayed activity occurring in the subendocardium and midmyocardium, a finding that would not have been apparent by epicardial mapping alone. The length of the reentrant pathway ranged from 1.8-3.0 cm. Marked delay was a necessary, but not a sufficient, condition for reentry to occur since, in some cases, delays as large as 220 msec was found without initiation of reentry or the occurrence of nonreentrant PVCs or VT. Maintenance of VT by intramural reentry arose in either the subendocardium or the subepicardium and was primarily dependent on the continued presence of marked transmural delay (159 +/- 8 msec). In contrast, in 24% of cases (5 of 21), initiation of the first beat of VT arose in either the subendocardium or subepicardium by a mechanism other than reentry as evidenced by the lack of intervening electrical activity between the end of the preceding sinus beat and the initiation of the ectopic beat. The preceding sinus beat was characterized by delay (129 +/- 12 msec) comparable to that of sinus beats preceding reentrant ectopic beats (p = NS), but the marked delay was distant from the site of nonreentrant initiation. Ventricular tachycardia could be initiated by one mechanism (reentrant or nonreentrant) and maintained or terminated by another mechanism.(ABSTRACT TRUNCATED AT 400 WORDS)
A CD46-binding Chimpanzee Adenovirus Vector as a Vaccine Carrier
Authors: Nia Tatsis, Ariella Blejer, Marcio O Lasaro, Scott E Hensley, Ann Cun, Lello Tesema, Yan Li, Guang-Ping Gao, Zhi Q Xiang, Dongming Zhou, James M Wilson, Hildegund C J Ertl
Journal: Molecular Therapy (2007)

A replication-defective chimeric vector based on the chimpanzee adenovirus serotype C1 was developed and tested as a vaccine carrier in mice. The AdC1 virus is closely related to human adenoviruses of subgroup B2 and uses CD46 for cell attachment. To overcome poor growth of E1-deleted AdC1 vectors on cell lines that provide the E1 of adenovirus of the human serotype 5 (AdHu5) virus in trans, the inverted terminal repeats and some of the early genes of AdC1 were replaced with those from AdC5, a chimpanzee origin adenovirus of subfamily E. The chimeric AdC1/C5 vector efficiently transduces CD46-expressing mouse dendritic cells (DCs) in vitro and initiates their maturation. Transduction of DCs in vivo is inefficient in CD46 transgenic mice. The AdC1/C5 vector induces transgene product-specific B- and CD8(+) T-cell responses in mice. Responses are slightly higher in wild-type mice than in CD46 transgenic mice. Transgene product-specific T-cell responses elicited by the AdC1/C5 vector can be increased by priming or boosting with a heterologous adenovirus vector. Pre-existing immunity to adenovirus of the common human serotype 5 does not affect induction of cell-mediated immune responses by the AdC1/C5 vector. This vector provides an additional tool in a repertoire of adenovirus-based vaccine vectors.
Some studies with a monoclonal antibody directed against human fibrinogen
Authors: Susan E. Francis, Douglas E. Joshua, Thomas Exner, Harry Kronenberg
Journal: American Journal of Hematology (1985)

Some properties of a monoclonal antibody generated against the fibrinogen component of a factor VIII preparation were investigated. The antibody bound with equal affinity in solid phase radioimmunoassays to fibrinogens isolated from both normal patients and patients with von Willebrand disease. It reacted in a sensitive immunoassay of plasma fibrinogen. The specificity of the antibody was confined to the parent molecule with no significant inhibition of fibrinogen binding by the fibrinogen degradation products (FDPs) X, Y, D, or E. The antibody had no significant effect on the activated partial thromboplastin time and prothrombin time of normal plasma. However, it prolonged the thrombin time as determined by the Clauss chronometric fibrinogen method. During fibrinogen lysis by plasmin immunoreactivity of fibrinogen to the antibody was lost at the same rate as the clottable fibrinogen content determined by the Clauss assay. The lack of reactivity of the antibody with FDPs makes it a suitable reagent for investigating plasmin activity as well as studying fibrinogen and fibrin. These findings suggest that the epitope of the antibody lies within the polar protruberance of the carboxy terminal end of the A alpha chain of fibrinogen and is destroyed by plasmin cleavage.