tbuckley/cabot-search huggingface dataset:
RECONSTITUTION OF 7S MOLECULES FROM L AND H POLYPEPTIDE CHAINS OF ANTIBODIES AND γ-GLOBULINS
Authors: D. E. Olins, G. M. Edelman
Journal: The Journal of Experimental Medicine (1964)

Admixture of separated L and H polypeptide chains of 7S gamma-globulins under appropriate conditions has been found to result in the reconstitution of 7S molecules. The chains were mixed in 0.5 N propionic acid and when dialyzed into neutral aqueous buffers interacted to form reconstituted material in greater than 30 per cent yield. This material had sedimentation coefficients of 6S to 7S, a weight average molecular weight of 160,000, and its antigenic structure and electrophoretic properties were the same as those of 7S gamma-globulin. By the use of I(131) and I(125) labels on the different types of chains, combined with ultracentrifugation of chain mixtures in sucrose density gradients, the 7S product was found to contain both isotopes in ratios consistent with the presence of two L and two H chains. After hydrolysis with papain, the reconstituted material yielded products resembling S and F fragments. All of the isotope corresponding to L chains was found in the counterpart of the S fragment; the isotope corresponding to the H chain fraction was present in both fragments. The activity reconstituted from chains of a purified guinea pig antibody to f1 phage was found to be associated mainly with the 7S material. Hybrid molecules containing rabbit L chains and human H chains and of human L chains and rabbit H chains were formed by the same techniques of reconstitution. It was found that the interchain disulfide bonds of native 7S gamma-globulins could be broken and reoxidized, as could those of reconstituted 7S material. Reduced L and H chains mixed in propionic acid, dialyzed against neutral buffers containing mercaptan, then against neutral buffers in the absence of mercaptan, formed stable 7S molecules of molecular weight 160,000, which were dissociable only after reduction.
Prognostic Role of the Histologic Subtypes of Renal Cell Carcinoma after Slide Revision
Authors: Vincenzo Ficarra, Guido Martignoni, Antonio Galfano, Giacomo Novara, Stefano Gobbo, Matteo Brunelli, Maurizio Pea, Filiberto Zattoni, Walter Artibani
Journal: European Urology (2006)
Update in Geriatrics
Authors: William J. Hall
Journal: Annals of Internal Medicine (1996)

Updates1 September 1996Update in GeriatricsWilliam J. Hall, MDWilliam J. Hall, MDFrom the University of Rochester, Rochester, New York.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-125-5-199609010-00006 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Every internist recognizes that the medical care of older persons is becoming increasingly central to clinical practice. Keeping up in this discipline requires somewhat unconventional approaches because the reports of exciting and useful clinical trials are scattered throughout the medical and surgical literature. This Update discusses a representative group of studies that focus on common problems seen in medical offices, care of hospitalized older persons, and clinical problems frequently encountered in nursing homes.Common Problems in Medical OfficesAdvances in office practices for the care of elderly patients have focused on preventing osteoporosis, fractures, and coronary artery disease and on ...References1. Cummings SR, Black DM, Rubin SM. Lifetime risks of hip, Colles', or vertebral fracture and coronary heart disease among white postmenopausal women. Arch Intern Med. 1989; 149:2445-8. Google Scholar2. Harris ST, Gertz BJ, Genant HK, Eyre DR, Survill TT, Ventura JN, et al. The effect of short term treatment with alendronate on vertebral density and biochemical markers of bone remodeling in early postmenopausal women. J Clin Endocrinol Metab. 1993; 76:1399-406. Google Scholar Author, Article, and Disclosure InformationAffiliations: From the University of Rochester, Rochester, New York.Corresponding Author: William J. Hall, MD, Department of Medicine, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Avenue, Box MED, Rochester, NY 14642.Current Author Addresses: Dr. Hall: Department of Medicine, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Avenue, Box MED, Rochester, NY 14642. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByAn implication of the short physical performance battery (SPPB) as a predictor of abnormal pulmonary function in aging peopleThe short physical performance battery as a predictor for long term disability or institutionalization in the community dwelling population aged 65 years old or olderPhysical Performance Measures in the Clinical Setting 1 September 1996Volume 125, Issue 5Page: 390-397KeywordsBone densityDisabilitiesElderlyHip fracturesHospitalizationsMedical risk factorsMedicareNSAIDsNursing homesWeight loss Issue Published: 1 September 1996 CopyrightCopyright © 1996 by American College of Physicians. All Rights Reserved.PDF DownloadLoading ...
Translating MicroRNA Discovery Into Clinical Biomarkers in Cancer
Authors: Scott A. Waldman, Andre Terzic
Journal: JAMA (2007)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Breast Cancer: Minimalists, Maximalists, Other Neoplasms
Authors: Michael J. Quinn
Journal: JAMA (1991)

<h3>To the Editor. —</h3> Schapira and Urban<sup>1</sup>raise an important question regarding whether our intuition and desire to do<i>more</i>is leading us to serve our patients well. I found the Wertheimer<sup>2</sup>commentary in response extremely disappointing. A factual error in the first paragraph casts doubt on the reliability of other data presented: "Approximately 10% of women in the United States (150 000) will be newly diagnosed in 1990 as having breast cancer...." (The number 150 000 is correct, but the percentage quoted would suggest that there are 1.5 million women in the United States, a number not consistent with a US population of 250 million.<sup>3</sup>) Wertheimer mounts arguments against straw men. For example, he lists reasons for follow-up of patients with breast cancer other than for assessment of the original breast cancer. Citing such a list suggests that Schapira and Urban discouraged such care, which is
Distinguishing Hemorrhagic Stroke From Ischemic Stroke—Reply
Authors: Shauna Runchey, Steven McGee
Journal: JAMA (2010)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
THIRD CASE OF MYCETOMA
Authors: Gustav A. Pagenstecher
Journal: JAMA (1924)

In 1922 I<sup>1</sup>reported two cases of mycetoma. Since then I have seen a third: A married Mexican woman, aged 36, born in Guanajuato of parents who were born and lived there, resided in Mexico until 21, when she moved to Lockhardt, Texas, and engaged in farm labor. Five years after coming to Texas, while picking cotton she fell and injured her right foot in the region of the lateral aspect of the ankle, and from this injury she dates the onset of the disease. The injury did not break the skin, and there was no visible evidence of injury. Two months after the injury, the patient's foot began to swell and pain her. These symptoms had been progressive since that time. After two years of gradual swelling, nodules appeared on the dorsum of the foot, and two years after the nodules appeared, the sinuses made their appearance in
Norfloxacin and Neutropenia
Authors: LUCIO PATOIA
Journal: Annals of Internal Medicine (1987)

Letters and Corrections1 November 1987Norfloxacin and NeutropeniaLUCIO PATOIA, M.D., ROBERTO GUERCIOLINI, M.D., FRANCESCO MENICHETTI, M.D., GlAMPAOLO BUCANEVE, M.D., ALBANO DEL FAVERO, M.D.LUCIO PATOIA, M.D.Search for more papers by this author, ROBERTO GUERCIOLINI, M.D.Search for more papers by this author, FRANCESCO MENICHETTI, M.D.Search for more papers by this author, GlAMPAOLO BUCANEVE, M.D.Search for more papers by this author, ALBANO DEL FAVERO, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-107-5-788_3 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: Norfloxacin is a fluoro-quinolone used to treat urinary tract infections (1). Although decreased leukocyte or neutrophil counts have been reported in about 1% of patients receiving norfloxacin (2), single case reports documenting this type of side effect have not been published.A 24-year-old woman was hospitalized because of anorexia nervosa. The biochemical analysis at admission showed slight normochromic normocytic anemia (hemoglobin, 12g/100 mL), normal leukocyte count (5600/mm3, with normal differential count), hypoalbuminemia (3g/100 mL), and raised alanine and aspartate aminotransferase levels (two to three times the normal values). No other laboratory abnormalities were seen.Because of urinary...References1. Norfloxacin. In: MC EVOY GK, DI PIETRO-HEYDORN J, PERRY MD, SHUPALA MK, eds. American Hospital Formulary Service. 24th ed. Bethesda: American Society of Hospital Pharmacists; 1987: supplement A, 18A-23A. Google Scholar2. SCHWABELIPPECHANGPOPSYAGER ABRMJ. Anorexia nervosa. Ann Intern Med. 1981;94:371-81. LinkGoogle Scholar3. BOWERSECKERT TE. Leukopenia in anorexia nervosa: lack of increased risk of infection. Arch Intern Med. 1978;138:1520-3. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: University of Perugia Perugia Italy PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByA Case of Severe Neutropenia From Short-Term Exposure to MoxifloxacinFluoroquinolonesMoxifloxacin-associated neutropeniaANTIBACTERIAL DRUGSMoxifloxacin-Associated Neutropenia in a Cirrhotic Elderly Woman with Lower Extremity CellulitisFluoroquinolonesTrovafloxacin-Associated LeukopeniaOfloxacin versus Vancomycin/Polymyxin for prevention of infections in granulocytopenic patientsFluoroquinolones 1 November 1987Volume 107, Issue 5Page: 788-789KeywordsAlanineAnemiaAnorexia nervosaHemoglobinNeutropeniaNeutrophilsResearch laboratoriesSerum glutamic oxaloacetic transaminase Issue Published: 1 November 1987 PDF DownloadLoading ...
Original antigenic sin and apoptosis in the pathogenesis of dengue hemorrhagic fever
Authors: Juthathip Mongkolsapaya, Wanwisa Dejnirattisai, Xiao-ning Xu, Sirijitt Vasanawathana, Nattaya Tangthawornchaikul, Aroonrung Chairunsri, Siraporn Sawasdivorn, Thaneeya Duangchinda, Tao Dong, Sarah Rowland-Jones, Pa-thai Yenchitsomanus, Andrew McMichael, Prida Malasit, Gavin Screaton
Journal: Nature Medicine (2003)
The Influence of Human Immunodeficiency Virus (HIV) Infection on Antibody Responses to Influenza Vaccines
Authors: Kenrad E. Nelson
Journal: Annals of Internal Medicine (1988)

Study Objective: To ascertain whether subjects infected with human immunodeficiency virus (HIV) generally develop protective hemagglutination inhibition antibody responses to inactivated influenza vaccines. Design: Prospective study of 104 persons before and after immunization. Setting: Outpatient clinic and hospital ward. Patients: Persons with the acquired immunodeficiency syndrome (AIDS) (n = 25), AIDS-related complex (n = 14), and HIV-seropositive men with only lymphadenopathy or no symptoms (n = 27). Controls were HIV-seronegative homosexual men (n = 22) and HIV-seronegative heterosexuals (n = 16). Interventions: Subjects were immunized with inactivated vaccines containing 15 μg of each of the following influenza virus hemagglutinins: A/Taiwan/1/86 (HINI), A/Mississippi/1/85(H3N2), A/Chile/1/83 (HINI), and B/Ann Arbor/1/86. Measurements and Main Results: Fourfold or greater antibody responses occurred less frequently in subjects with HIV infections than in HIV-seronegative controls. Protective levels (1:64 or greater) of hemagglutination inhibition antibodies were attained by 94% to 100% of HIV-seronegative controls, 52% to 89% of HIV-seropositive asymptomatic subjects, and 13% to 50% of subjects with AIDS or AIDS-related complex. No increase in the prevalence or level of serum HIV p24 antigen or clinical deterioration was detected among HIV-infected persons after influenza immunization. Conclusions: Because of the poor antibody responses to influenza vaccines among HIV-infected subjects, even in many with no or minimal symptoms, alternative strategies for preventing influenza, such as booster doses of influenza vaccine, prophylaxis with amantidine, or both should be considered.
Male-Driven Evolution of Mitochondrial and Chloroplastidial DNA Sequences in Plants
Authors: Carrie-Ann Whittle, Mark O. Johnston
Journal: Molecular Biology and Evolution (2002)

Although there is substantial evidence that, in animals, male-inherited neutral DNA evolves at a higher rate than female-inherited DNA, the relative evolutionary rate of male- versus female-inherited DNA has not been investigated in plants. We compared the substitution rates at neutral sites of maternally and paternally inherited organellar DNA in gymnosperms. The analysis provided substantial support for the presence of a higher evolutionary rate in both the mitochondrial and chloroplastidial DNA when the organelle was inherited paternally than when inherited maternally. These results suggest that, compared with eggs, sperm tend to carry a greater number of mutations in mitochondrial and chloroplastidial DNA. The existence of a male mutation bias in plants is remarkable because, unlike animals, the germ-lines are not separated from the somatic cells throughout an individual's lifetime. The data therefore suggest that even a brief period of male and female germ-line separation can cause gender-specific mutation rates. These results are the first to show that, at least in some species, germ-lines influence the number of mutations carried in the gametes. Possible causes of male mutation bias in plants are discussed.
COMPOSITION OF IMPORTED BOTTLED WATERS
Authors: A. E. MIX
Journal: JAMA (1925)

During the fifteen years prior to the beginning of the World War in 1914, the annual receipts of foreign mineral waters amounted to about 3,000,000 gallons, valued at about $1,000,000. There was a marked decrease in receipts during the World War and for several years thereafter, but since 1920 there has been a gradual increase so that at the present time we are. receiving about one-fourth the quantity imported prior to the World War. During the twelve months ending in June, 1924, there were imported 753,831 gallons, valued at $281,402.<sup>1</sup>The value of domestic waters sold annually during the last twenty-five years has remained about the same, that is, in the neighborhood of $5,000,000, so that possibly the value of imported waters may in the course of time rise to the former figure of $1,000,000 annually. In a study of the composition of a large number of mineral waters,
Helper T cell-dependent human B cell differentiation mediated by a mycoplasmal superantigen bridge.
Authors: J R Tumang, D N Posnett, B C Cole, M K Crow, S M Friedman
Journal: The Journal of Experimental Medicine (1990)

Experimentally induced murine graft-vs.-host disease may be characterized by hypergammaglobulinemia, autoantibody formation, and immune complex-mediated organ system damage that mimics SLE. These autoimmune phenomena are mediated by abnormal Th-B cell cooperation, across MHC disparities, in which donor-derived allospecific Th cells recognize and interact with MHC class II antigens on the surface of recipient B cells. Microbial toxins, termed superantigens, which bind to MHC class II molecules and activate selected T cells based on TCR variable gene usage, may induce a similar form of Th-B cell interaction. In the present study, we generated and characterized human Th cell lines reactive with the Mycoplasma arthritidis superantigen (MAM). The essential observation is that resting human B cells bind MAM and present it to superantigen-reactive autologous or allogeneic Th cells, resulting in both Th cell activation and a consequent polyclonal Ig response by the superantigen-bearing B cells.
Diagnostic Cytology and Its Histopathologic Bases
Authors: George L. Wied
Journal: JAMA (1961)

The outstanding features of this book are the comparative cytological and histological findings, the concise and instructive text, and the numerous and generally excellent photomicrographs. It is the sincere feeling of this reviewer that no cytopathologist and no cytology laboratory, as well as no cytologically interested clinician, should be without this book. The publishers should be congratulated for the excellent composition and illustrations (black-and-white as well as colored photographs). Translations into other languages should be seriously considered in the near future. The authors have given us a needed and lasting addition to our cytological literature with this important book.
High ICAM-1 gene expression in pulmonary fibroblasts of COPD patients: a reflection of an enhanced immunological function
Authors: A. Zandvoort, Y. M. van der Geld, M. R. Jonker, J. A. Noordhoek, J. T. W. M. Vos, J. Wesseling, H. F. Kauffman, W. Timens, D. S. Postma
Journal: European Respiratory Journal (2006)

Chronic obstructive pulmonary disease (COPD) is characterised by destruction of extracellular matrix (ECM) in parenchymal areas, whereas the bronchial walls can show fibrosis. In addition, an extensive inflammatory process is observed. CD8+ T-cells, located throughout the lung, and epithelial cells in centrally located airways, produce cytokines involved in the inflammatory process. These cytokines may influence the present fibroblasts, the key effectors in the defective ECM repair and maintenance in COPD. The current authors explored the effects of the cytokine microenvironment on cell–cell interaction gene expression in pulmonary fibroblasts of controls (n = 6), and Global Initiative for Chronic Obstructive Lung Disease stage II (n = 7) and stage IV (n = 7) COPD patients. The current authors simulated the in vivo microenvironment using supernatants of CD3/CD28 stimulated CD8+ T-cells isolated from peripheral blood of COPD patients, supernatant of a bronchial-epithelial cell line, or a combination of both. The present data show that fibroblasts of chronic obstructive pulmonary disease patients display an altered response to the cytokine microenvironment, depending on both the disease stage and the central or peripheral location in the lung. Especially adhesion-related genes are upregulated in fibroblasts of chronic obstructive pulmonary disease patients, which can indicate a more pronounced role of fibroblasts in the inflammatory process in chronic obstructive pulmonary disease, possibly resulting in reduced function as effectors of extracellular matrix repair.
Farnesyltransferase Inhibitors Reverse Taxane Resistance
Authors: Adam I. Marcus, Aurora M. O'Brate, Ruben M. Buey, Jun Zhou, Shala Thomas, Fadlo R. Khuri, Jose Manuel Andreu, Fernando Díaz, Paraskevi Giannakakou
Journal: Cancer Research (2006)

Abstract The combination of farnesyltransferase inhibitors (FTIs) and taxanes has been shown to result in potent antiproliferative and antimitotic synergy. Recent phase I and II clinical trials have shown that this combination shows clinical activity in taxane-refractory or taxane-resistant cancer patients. To understand the mechanism behind these clinical observations, we used a cancer cell model of paclitaxel resistance and showed that the FTI/taxane combination retains potent antiproliferative, antimitotic, and proapoptotic activity against the paclitaxel-resistant cells, at doses where each drug alone has little or no activity. To probe the mechanistic basis of these observations, paclitaxel activity was monitored in living cells using the fluorescently conjugated paclitaxel, Flutax-2. We observed that all FTIs tested increase the amount of microtubule-bound Flutax-2 and the number of microtubules labeled with Flutax-2 in both paclitaxel-resistant and paclitaxel-sensitive cells. Importantly, we observed a consequential increase in microtubule stability and tubulin acetylation with the combination of the two drugs, even in paclitaxel-resistant cells, confirming that the enhanced taxane binding in the presence of FTI affects microtubule function. Furthermore, this mechanism is dependent on the function of the tubulin deacetylase, HDAC6, because in cells overexpressing a catalytically inactive HDAC6, FTIs are incapable of enhancing Flutax-2–microtubule binding. Similar results were obtained by using an FTI devoid of farnesyltransferase inhibitory activity, indicating that functional inhibition of farnesyltransferase is also required. Overall, these studies provide a new insight into the functional relationship between HDAC6, farnesyltransferase, and microtubules, and support clinical data showing that the FTI/taxane combination is effective in taxane-refractory patients. (Cancer Res 2006; 66(17): 8838-46)
Targeting ATM ameliorates mutant Huntingtin toxicity in cell and animal models of Huntington’s disease
Authors: Xiao-Hong Lu, Virginia B. Mattis, Nan Wang, Ismael Al-Ramahi, Nick van den Berg, Silvina A. Fratantoni, Henry Waldvogel, Erin Greiner, Alex Osmand, Karla Elzein, Jingbo Xiao, Sipke Dijkstra, Remko de Pril, Harry V. Vinters, Richard Faull, Ethan Signer, Seung Kwak, Juan J. Marugan, Juan Botas, David F. Fischer, Clive N. Svendsen, Ignacio Munoz-Sanjuan, X. William Yang
Journal: Science Translational Medicine (2014)

Reducing ATM signaling is neuroprotective in cell and animal models of Huntington’s disease.