tbuckley/cabot-search huggingface dataset:
Local Delivery of Human Tissue Kallikrein Gene Accelerates Spontaneous Angiogenesis in Mouse Model of Hindlimb Ischemia
Authors: Costanza Emanueli, Alessandra Minasi, Antonella Zacheo, Julie Chao, Lee Chao, Maria Bonaria Salis, Stefania Straino, Maria Grazia Tozzi, Robert Smith, Leonardo Gaspa, Giuseppe Bianchini, Francesco Stillo, Maurizio C. Capogrossi, Paolo Madeddu
Journal: Circulation (2001)

Background —Human tissue kallikrein (HK) releases kinins from kininogen. We investigated whether adenovirus-mediated HK gene delivery is angiogenic in the context of ischemia. Methods and Results —Hindlimb ischemia, caused by femoral artery excision, increased muscular capillary density ( P &lt;0.001) and induced the expression of kinin B 1 receptor gene ( P &lt;0.05). Pharmacological blockade of B 1 receptors blunted ischemia-induced angiogenesis ( P &lt;0.01), whereas kinin B 2 receptor antagonism was ineffective. Intramuscular delivery of adenovirus containing the HK gene (Ad.CMV-cHK) enhanced the increase in capillary density caused by ischemia (969±32 versus 541±18 capillaries/mm 2 for control, P &lt;0.001), accelerated blood flow recovery ( P &lt;0.01), and preserved energetic charge of ischemic muscle ( P &lt;0.01). Chronic blockade of kinin B 1 or B 2 receptors prevented HK-induced angiogenesis. Conclusions —HK gene delivery enhances the native angiogenic response to ischemia. Angiogenesis gene therapy with HK might be applicable to peripheral occlusive vascular disease.
Abnormal Parasympathetic Vagal Function in Patients with Spasmodic Dysphonia
Authors: MARK FELDMAN
Journal: Annals of Internal Medicine (1984)

Gastric vagal function was assessed in 15 patients with spasmodic dysphonia by measuring gastric acid output in response to sham feeding. Patients secreted significantly less acid than controls (p < 0.001). Cardiac vagal function was assessed in 11 patients by measuring heart rate during deep respiration and also during and after Valsalva maneuver. Patients with spasmodic dysphonia had a significantly reduced fluctuation of heart rate during deep respiration (sinus arrhythmia). The expiratory to inspiratory R-R interval averaged 1.08 ± 0.08 (mean ± SD) in patients and 1.22 ± 0.10 in controls (p < 0.005). The ratio of tachycardia during Valsalva maneuver to bradycardia after Valsalva manuever was also lower in patients than in controls (p < 0.005). The auditory brainstem response was abnormal in 11 of 15 patients. Our results show either a central brainstem abnormality or several cranial nerve abnormalities in some patients with spasmodic dysphonia.
Vitamin D3–Triggered Antimicrobial Response—Another Pleiotropic Effect beyond Mineral and Bone Metabolism
Authors: P.T. Liu, S. Stenger, H. Li, L. Wenzel, B.H. Tan, S.R. Krutzik, M.T. Ochoa, J. Schauber, K. Wu, C. Meinken, D.L. Kamen, M. Wagner, R. Bals, A. Steinmeyer, U. Zugel, R.L. Gallo, D. Eisenberg, M. Hewison, B.W. Hollis, J.S. Adams, B.R. Bloom, R.L. Modlin
Journal: Journal of the American Society of Nephrology (2006)

![Figure][1]</img> Eberhard Ritz Feature Editor Niels Ryberg Finsen, who received the third Nobel prize in medicine in 1903, was honored because in 1895 he had discovered a cure for a disease that had been incurable before, i.e. , skin tuberculosis known as lupus vulgaris ([1][2]–[3][3]).
Immunotherapy for Human Renal Cell Carcinoma by Adoptive Transfer of Autologous Transforming Growth Factor β–Insensitive CD8+ T Cells
Authors: Longxin Wang, Weihong Wen, Jianlin Yuan, Brian Helfand, Yu Li, Changhong Shi, Feng Tian, Jia Zheng, Fuli Wang, Lin Chen, Lili Liang, Liqun Zhou, Chung Lee, Zhinan Chen, Yinglu Guo, He Wang, Qiang Zhang, Weijun Qin
Journal: Clinical Cancer Research (2009)

Transforming growth factor-beta (TGF-beta) is a potent immunosuppressor that has been associated with tumor evasion from the host immune surveillance and, thus, tumor progression. We tested a novel immunotherapy for human renal cell cancer (RCC) using a technique that involves the adoptive transfer of autologous tumor-reactive, TGF-beta-insensitive CD8(+) T cells into human RCC-challenged immunodeficient mice to identify its potent antitumor responses.The present study was conducted using a one-to-one adoptive transfer strategy to treat tumor-bearing severe combined immunodeficient (SCID/beige) mouse. The SCID/beige mice were humanized with peripheral blood mononuclear cells from patients with RCC (Hu-PBMC-SCID) before adoptive transfer. Autologous CD8(+) T cells were expanded ex vivo using autologous patient's dendritic cells pulsed with the tumor lysate and rendered TGF-beta insensitive by dominant-negative TGF-beta type II receptor. In addition, human RCC cell lines were generated using patients' tumor cells injected into SCID/beige mice.Using flow cytometry analysis, we confirmed the expression of the tumor-reactive, TGF-beta-insensitive CD8(+) T cells were the effector CD8(+) cells (CD27(-)CDRA(+)). Adoptive transfer of autologous TGF-beta-insensitive CD8(+) T cells into tumor-bearing Hu-PBMC-SCID mice induced robust tumor-specific CTL responses in vitro, were associated with tumor apoptosis, suppressed lung metastasis, and prolonged survival times in vivo.The one-to-one adoptive transfer strategy is an ideal in vivo murine model for studying the relationship between TGF-beta and immunosurveillance in RCC in vivo. Furthermore, this technique may offer the promise of a novel therapeutic option for the treatment of human patients with RCC.
Bereavement: Its Psychosocial Aspects
Authors: Juliana Geran Pilon
Journal: JAMA (1976)

This collection of articles—funded by the Foundation of Thanatology and various government grants—is intended primarily to persuade us of the need for more research and "professional" counseling in thanatology. To educate the public on the problems related to this field, the essays deal with issues such as the importance of grief in the growth process, the physiological effects of bereavement, special problems of widows and widowers, withdrawal in orphaned children, and reactions to unexpected or untimely death. An entire section is devoted to the role of health professionals and another to therapeutic intervention in bereavement. Its volume and range of subjects notwithstanding, this book is fast reading. The findings, in all too many instances, amount to little more than common sense. Item: "more spouses and parents tended to have intense reactions [to the death] than did other relatives"; or "sleep disturbances and weight loss [were observed] in the widowed in
Dovitinib synergizes with oxaliplatin in suppressing cell proliferation and inducing apoptosis in colorectal cancer cells regardless of RAS-RAF mutation status
Authors: Shikha Gaur, Linling Chen, Vincent Ann, Wei-Chen Lin, Yafan Wang, Vincent HS Chang, Nan Yong Hsu, Her-Shuyong Shia, Yun Yen
Journal: Molecular Cancer (2014)

Cancer is the result of a multistep process of genomic alterations, including mutations in key regulatory proteins that result in loss of balanced gene expression and subsequent malignant transformation. Throughout the various stages of colorectal carcinoma (CRC), complex genetic alterations occur, of which over-expression of growth factors, such as vascular endothelial growth factor, fibroblast growth factor and platelet-derive growth factor and their corresponding receptor tyrosine kinases, have been shown to correlate with invasiveness, tumor angiogenesis, metastasis, recurrence, and poor prognosis of colorectal cancer. To evaluate the therapeutic effect, we combined Dovitinib, an orally bioavailable, potent inhibitor of class III-V receptor tyrosine kinases with chemotherapeutic drug, oxaliplatin in preclinical models of colon cancer.Human colon cancer cells with different RAS-RAF mutation status (HCT-116, HT-29, SW-480, CaCO2 and LS174T) were treated with a combination of Dovitinib and Oxaliplatin at low dosage followed by assays to investigate the effect of the combination on cell proliferation, cell migration, cell apoptosis and signaling pathways involved in molecular mechanism of drug(s). The antitumor effects of either of the drugs were compared to the combination using human colon carcinoma cell line HT-29 xenograft model. Treated vs untreated tumor sections were also compared for proliferation and angiogenesis markers by immunohistochemistry.The combination of dovitinib and oxaliplatin showed higher in vitro cytotoxicity in colon cell lines irrespective of their RAS-RAF status as compared to either of the drugs alone. Simultaneous inhibition of MAP kinase and AKT pathways and induction of apoptosis via activation of caspases 9/caspases 3 contributed to the synergistic effect of this combination therapy. In the xenograft model, the combination showed a significantly higher antitumor activity. Immunohistochemistry of post treatment tumors showed a significant decrease in proliferation and angiogenesis as compared to either of the treatments alone.This study demonstrates the synergistic antitumor activity of combination of dovitinib and oxaliplatin against colon cancer with different RAS-RAF status. The combination also showed its antitumor efficacy in a multidrug resistant phenotype xenograft model. This provides a basis for further investigation for its potential in clinical setting for colorectal cancer.
Impaired atrial receptor modulation or renal nerve activity in dogs with chronic volume overload1
Authors: I. H ZUCKER, A. J GORMAN, K. G CORNISH, M. LANG
Journal: Cardiovascular Research (1985)

Left atrial receptors with vagal afferents are reset and become less sensitive in dogs with chronic high output congestive heart failure (Zucker et al J Clin Invst 1977;60:323). We determined if the normal reflex inhibition of renal sympathetic nerve activity (RNA) which occurs in response to left atrial distension is altered in dogs with chronic volume overload produced by an aorto-vena caval fistula (AVF). In addition, we examined the ability of the arterial baroreflex to alter RNA. In normal dogs, left atrial distension decreased RNA by 6.8±1.8% per mmHg change in left atrial pressure (LAP). After carotid sinus denervation (CSD), this inhibition was increased to 10.9±1.4% per mmHg change in RNA in response to left atrial distension. Vagotomy completely abolished any change in RNA in response to left atrial distension. In contrast, dogs with chronic AVF's demonstrated an increase in RNA in response to left atrial distension by 4.5 ±3.9% per mmHg change in LAP. The response of the AVF dogs was significantly different from the normal dogs (p<0.005). This was also the case after CSD. Loading and unloading of the arterial baroreceptors with norepinephrine and nitroprusside demonstrated that the modulation of RNA by arterial baroreceptors was similar in AVF dogs compared with normal dogs. The present study demonstrates that chronic volume overload results in a decrease in the peripheral sympathetic inhibitory effects of left atrial receptor stimulation at a time when arterial baroreceptor function is preserved.
COLORED CHILDREN: A PSYCHOPHYSICAL STUDY.
Authors: ARTHUR MACDONALD
Journal: JAMA (1899)

In all new lines of inquiry it is a general principle to regard the results as more or less tentative, according to the number of persons experimented upon. In this study the results depend upon averages which are valuable according to the whole numbers from which the averages are made. In many instances these numbers are not as large as one would desire. We hope this may induce some investigator to make experiments upon larger numbers. <h3>SPECIAL STUDY OF NINETY-ONE COLORED CHILDREN.</h3> Instruments used: The calipers (Fig. 1) are used for measuring the length and width of head. The right end of the calipers (R) is held at the root of the nose; the one measuring watches the scale while he brings the left point of the calipers over the back and middle of the head, thus finding the maximum length of head. The width of the head is measured
Orderly arrayed deposit of urate crystals in gout suggest epitaxial formation
Authors: E. PASCUAL, S. ORDONEZ
Journal: Annals of the Rheumatic Diseases (1998)

hypothalamus or hypophysis stalk.8][9] It could be that the patients presented here had vascular impairment, not because of inflammatory causes, but because of thrombotic events.Finally we cannot rule out a direct autoimmune lesion.Schgeerbaum and Botazzo 10 published the existence of autoantibodies against vasopressin producing neurons in some patients suVering from NDI.However, the antibodies against hypophysis structures were negative by IIF in our two cases.
A Simple Position to Provide Better Imaging of Upper Ureteral Stones Close to the Crista Iliaca during Extracorporeal Shock Wave Lithotripsy Using the Siemens Lithostar
Authors: Nejat Tansu, Can Öbek, Bülent Önal, Veli Yalçın, Armağan Öner, Vural Solok
Journal: European Urology (2003)
Oral acylovir in herpes simplex virus associated erythema multiforme
Authors: Renata Strumia
Journal: British Journal of Dermatology (1988)

Journal Article Oral acylovir in herpes simplex virus associated erythema multiforme Get access Renata Strumia Renata Strumia Clinica Dermatologica, Università di Ferrara, 44100 Ferrara, Italy Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 118, Issue 3, 1 March 1988, Page 449, https://doi.org/10.1111/j.1365-2133.1988.tb02443.x Published: 01 March 1988
Allergy to topical corticosteroids in inflammatory bowel disease
Authors: B E Monk
Journal: Gut (2003)

A 57 year old Caucasian man presented with an eight week history of loose bowel motions 10 times daily, with blood, slime, and mucus. Sigmoidoscopy showed an abnormal vascular pattern and a granular mucosa. Rectal biopsy was reported histologically to show inflammatory bowel disease, and treatment was initiated with oral Asacol (mesalazine) 800 mg three times daily and Predfoam (prednisolone metasulphobenzoate sodium) enema twice daily. This treatment was continued over a period of about eight weeks, initially with some improvement but latterly relapsing to the pretreatment state. Fibreoptic colonoscopy was then performed, revealing inflammatory changes up to the distal sigmoid colon. Biopsies were suggestive of Crohn’s disease. Treatment was continued for a further three months without improvement. The patient decided on his own initiative to stop …
Intratracheal instillation versus intratracheal inhalation: influence of cytokines on inflammatory response.
Authors: M Osier, R B Baggs, G Oberdörster
Journal: Environmental Health Perspectives (1997)

Our laboratory has developed a method of particle exposure whereby anesthetized rats intratracheally inhale, at a regulated breathing rate and pressure, an aerosolized test material. This method is capable of delivering considerable doses in a short time period and, unlike the commonly used method of intratracheal instillation, does so with an even particle distribution throughout the lung. Early studies comparing the response of male Fischer 344 rats exposed to TiO2 particles of two differing primary particle sizes showed that at similar particle doses animals exposed by the two methods showed differences in response, as measured by bronchoalveolar lavage (BAL) parameters. Building on this, we sought to study the roles that macrophage inflammatory protein-2 (MIP-2) and tumor necrosis factor alpha (TNF-alpha), two cytokines thought to have proinflammatory roles in the lung, may play in the differences observed. Increases in MIP-2 protein levels in the lavaged cells, but not the supernatant, were observed in those groups where increased polymorphonuclear cells (PMN) in the lung lavage were found, but not in those where no increase in PMN levels was observed. BAL TNF-alpha levels, measured by enzyme-linked immunosorbent assay, showed no apparent correlation with cellular or biochemical BAL parameters for either particle size or dosing method. Increases in immunocytochemical staining for TNF-alpha, compared to unexposed controls, were observed in several particle-exposed groups. Thus, it appears that increased BAL MIP-2 protein levels, but not TNF-alpha, correlate well with the inflammatory response, as measured by PMN numbers in lavaged cells, for both exposure systems.
Causes and Control of Dental Caries
Authors: James H. Shaw
Journal: New England Journal of Medicine (1987)

DENTAL caries (tooth decay) is a chronic infectious disease in which the active agent or agents are members of the indigenous oral flora. Carious lesions result primarily from the dissolution of mineral in enamel and dentin by acids produced during metabolism of food residues by microorganisms colonizing tooth surfaces.1 2 3 When dentin, with its 20-fold higher protein content than enamel, is invaded, proteolysis and possibly chelation also occur.Numerous archaeological reports indicate that carious lesions were infrequent in humans during antiquity.4 , 5 In England, the frequency of caries was low as recently as Saxon times.6 Major increases occurred during the Middle Ages, . . .
Japanese B Encephalitis in an American Soldier Returning from Korea
Authors: Florentino Perez Pina, Urho R. Merikangas
Journal: New England Journal of Medicine (1953)

TO the best of our knowledge, no case of Japanese B encephalitis has previously been reported as occurring in personnel among Far East troops after evacuation to the United States. That this possibility is not far fetched is illustrated by the case reported below. Rapid evacuation by air from an endemic or epidemic zone of Japanese B encephalitis may result in recognition of other cases of this type in the continental United States.Case ReportA 25-year-old sergeant was admitted to Murphy Army Hospital on September 13, 1952, while on leave. His chief complaints were headache, beginning 4 days before . . .
Dilemma of a surgical trainee
Authors: Bettina Lieske
Journal: BMJ (2005)

On finishing my basic surgical training and after gaining membership of the Royal College of Surgeons, I was keen to proceed in my chosen career in general surgery as quickly as possible. I was shortlisted for specialist interviews in early summer and offered a training number in the Oxford Deanery. Everybody who has been through this process can imagine how happy I was. Proud of my achievement, I was looking forward to my training as an SpR.

My first appraisal went well. I am currently attached to the vascular firm, but since I am not aspiring to a career in vascular surgery and I am at the beginning of my training, the targets that my trainers identified for me include genuine surgical experience: By the end of my vascular attachment I “should be able to expose the groin vessels unsupervised, to have refined the performance of common femoral …
RATES OF ANTIBODY SYNTHESIS DURING FIRST, SECOND, AND HYPERIMMUNE RESPONSES OF RABBITS TO BOVINE GAMMA GLOBULIN
Authors: Frank J. Dixon, Paul H. Maurer, William O. Weigle, Maria P. Deichmiller
Journal: The Journal of Experimental Medicine (1956)

Determinations of the rates of antibody synthesis during first, second, and hyperimmune responses to bovine gamma globulin using S(35)-labelle aminod acids indicate the following: 1. In all three responses the rate of antibody synthesis increases while antigen is circulating and then begins to decline rapidly after elimination of detectable circulating antigen. 2. The initial rates of decline of antibody synthesis are approximately the same for all three responses. 3. There is a relatively persistent source of antibody production which appears after repeated stimulation and increases in proportion to the number of repeated stimuli.
Abstract 1449: Chronic consumption of a high fat diet increases solid tumor growth and lung metastasis in BALB/c mice subcutaneously injected with CT26 colon cancer cells
Authors: Heesook Park, Hyerim Song, Jung Han Yoon Park
Journal: Cancer Research (2010)

Abstract Epidemiological and laboratory animal studies suggest that dietary factors in general and specifically high dietary fat and calories increase the risk of colon cancer. In the present study, we determined whether dietary fat increases solid tumor growth and metastasis of colon cancer using a CT26 mouse colon cancer xenograft model. Four-week old, male BALB/c mice was fed a purified diet containing 34.9% (w/w) fat (high fat diet, HFD), or 4.3% fat (low fat diet, LFD) for a period of 16 weeks. After 16 weeks, CT26 colon cancer cells were injected subcutaneously into the animals’ right flank. 5 weeks after cell injection, animals were killed. The mean body weight was slightly increased in the HFD group (33.9 ± 0.46 g) as compared to the LFD group (31.9 ± 0.37 g). The weights of the liver, lung, and epididymal fat pad were also increased in animals fed the high fat diet. Solid tumor growth (tumor volumes and weights) and the number and volume of tumor nodules in the lung were markedly increased in the HFD group as compared to the LFD group. Immunohistochemical staining of tumor tissues revealed that the expression of proliferating cell nuclear antigen (PCNA), platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31, an angiogenesis marker), vascular endothelial growth factor, and phospho-tyrosine phosphatase (CD 45, a monocytes/macrophage marker) was markedly increased in the HFD group as compared to those in the LFD group. Results from cytokine array revealed that the expression of IFN-γ, IL-1ra, IL-16, CXCL9, MIP-2, CCL5, and TNF-α was up-regulated in the sera of the HFD group as compared to the LFD group. These results indicate that increases in angiogenesis, infiltration of monocytes and cytokine expression probably contribute to increased tumor growth and lung metastasis in mice fed the high fat diet. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1449.
Effect of genetic counselling on the prevalence of Huntington's chorea.
Authors: C O Carter, K A Evans, M Baraitser
Journal: BMJ (1983)

The relative fertility of sons and daughters of patients with Huntington's chorea was found to be a little under 0.5 if they had been told of their risk of transmitting the disease before they had started their families. The effect was much the same in those who had attended the genetic clinic at The Hospital for Sick Children on a single occasion and those who had been told of their risk directly, or indirectly through the patient's spouse or family doctor, by the neurologist who was looking after their affected parent at the National Hospital for Nervous Diseases. If all offspring of patients were informed of their risk the effect on the prevalence of the disorder would be substantial, especially if the mutation rate is low and the reproductive fitness of patients in the past has been close to 1.0. Men and women at risk of developing the disease should not be seen on just one occasion, however: they need continued support by being seen regularly at a special neurological genetic clinic.