tbuckley/cabot-search huggingface dataset:
The Impact of Angiotensin-Converting Enzyme Inhibitor Therapy on the Extracellular Collagen Matrix During Left Ventricular Assist Device Support in Patients With End-Stage Heart Failure
Authors: Stefan Klotz, A.H. Jan Danser, Robert F. Foronjy, Mehmet C. Oz, Jie Wang, Donna Mancini, Jeanine D’Armiento, Daniel Burkhoff
Journal: Journal of the American College of Cardiology (2007)
Reduced Dose of Primaquine Effective in Blocking Malaria Transmission
Authors: M. J. Friedrich
Journal: JAMA (2014)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Motion of the interatrial septum in acute mitral regurgitation. Clinical and experimental echocardiographic studies.
Authors: C Tei, H Tanaka, S Nakao, H Yoshimura, S Minagoe, T Kashima, T Kanehisa
Journal: Circulation (1980)

The interatrial septal echocardiograms from 15 patients with acute mitral regurgitation due to ruptured chordae tendineae were compared with those from 14 normal subjects. On the cross-sectional echocardiogram, the interatrial septal configuration in patients with chordal rupture showed a characteristic pattern in which the interatrial septum (IAS) was flat or slightly convex toward the left atrium at end-diastole and became markedly convex toward the right atrium at end-systole. On the M-mode echocardiogram, the interatrial septal amplitude was greater in patients with chordal rupture (12.4 +/- 1.9 mm) than in normal subjects (9.4 +/- 0.9 mm). Systolic fluttering of the IAS was found in five of 10 patients with rupture of the chordae attached to the posterior mitral leaflet. This finding was thought to be specific for acute mitral regurgitation due to ruptured chordae to the posterior mitral leaflet. After operation, the amplitude of the IAS became normal or diminished and systolic fluttering of the IAS disappeared. Animal experiments performed to clarify the mechanism of these findings showed that increased systolic motion of the IAS resulted from an increased in the systolic left atrial-to-right atrial pressure gradient due to acute mitral regurgitation. The systolic fluttering of the IAS was thought to represent a jet stream against the IAS due to rupture of the chordae tendineae to the lateral half of the posterior mitral leaflet. We conclude that the interatrial septal echocardiogram reflects the hemodynamic changes due to acute mitral regurgitation and direction of the regurgitant jet against the IAS. This finding may prove to be important in diagnosing acute mitral regurgitation secondary to ruptured chordae tendineae.
Development of Subaortic Stenosis After Pulmonary Artery Banding
Authors: MICHAEL D. FREED, AMNON ROSENTHAL, WILLIAM H. PLAUTH, ALEXANDER S. NADAS
Journal: Circulation (1973)

Palliation of children with large ventricular septal defects (VSD) by pulmonary artery banding (PAB) is associated with a variety of unfavorable sequelae. We document another possible complication of PAB, the development of subaortic stenosis, which occurred in 4 of 40 infants with large VSD and PAB. Prior to surgery all had congestive heart failure, cardiomegaly, pulmonary plethora, and biventricular or right ventricular hypertrophy on electrocardiogram. At cardiac catheterization all had large VSD with pulmonary artery hypertension. No gradient between left ventricle and systemic artery was demonstrated. PAB was followed by improvement in all four children. Repeat catheterization at 4 to 11 years of age revealed that they had developed a significant (30 to 100 mm Hg) gradient across the left ventricular outflow tract. Although the etiology of the subaortic stenosis is not known, it is felt that the hypertrophied and possibly leftward deviated conal septum was impinging on the left ventricular outflow tract in three of the four. Since the subaortic stenosis was not suspected prior to the second catheterization, we feel that preoperative evaluation of children with VSD and PAB should include evaluation of the left ventricular outflow tract to be certain that no obstruction is present.
Phase II randomized, double-blind, placebo-controlled study of AMG 386 (trebananib) in combination with cisplatin and capecitabine in patients with metastatic gastro-oesophageal cancer
Authors: M.M. Eatock, N.C. Tebbutt, C.L. Bampton, A.H. Strickland, M. Valladares-Ayerbes, A. Swieboda-Sadlej, E. Van Cutsem, N. Nanayakkara, Y.-N. Sun, Z.D. Zhong, M.B. Bass, A.H. Adewoye, G. Bodoky
Journal: Annals of Oncology (2012)
Definition of Primary and Secondary Glioblastoma—Letter
Authors: Peter Werner, Dominik Fritzsch, Heidrun Holland, Manfred Bauer, Wolfgang Krupp, Karl-Titus Hoffmann, Dorothee Saur, Florian Then Bergh, Osama Sabri, Henryk Barthel
Journal: Clinical Cancer Research (2014)

We read with great interest the article by Ohgaki and Kleihues (1) in which the molecular genetic concept of primary and secondary glioblastoma was reviewed. In response to this most interesting article, we want to present the first case in which the occurrence of an IDH1-negative glioblastoma was monitored over time by combined [11C] methionine (MET) amino acid transport PET/MRI (Fig. 1).This case underlines that the uncertainty in differential diagnosis between primary and secondary glioblastoma, despite the clinical definition and advanced imaging, can be overcome with molecular genetics; although in this case, there was evidence of a less malignant precursor lesion in positron emission tomography (PET), a secondary glioblastoma could be ruled out on the basis of the IDH1 profile.Ohgaki and Kleihues outline the high clinical importance of predicting malignant transformation in primary brain tumors. We would like to expand the molecular genetic perspective of their article by that of molecular imaging. In the present case, the 47% increase in amino acid transport observed over 6 weeks allowed ruling out a low-grade tumor. This is in keeping with other literature reporting high accuracy of amino acid brain tumor imaging for grading actrocytomas and evaluating their malignant transformation (2, 3). For the future, an incremental diagnostic value in the assessment of asctrocytomas can even be expected from multiparametric analyses of different imaging information as they are now for the first time obtainable within one session by combined PET/MRI (4, 5).See the Response, p. 2013M. Bauer, W. Krupp, and K.-T. Hoffmann have received speakers bureau honoraria for Bracco Imaging and Bayer Healthcare. O. Sabri and H. Barthel have received speakers bureau honoraria from Bayer Healthcare and Siemens Healthcare, and are consultant/advisory board members for Bayer Healthcare and Piramal Imaging. No potential conflicts of interest were disclosed by the other authors.This study was supported by the German Research Foundation (SA 669/9-1).
813. Distribution of Transgene Expressing Cells Following Intramarrow Injection to Transduce Hematopoietic Stem Cells In Situ Using rSV40 Vectors
Authors: Jean-Pierre Louboutin, Bianling Liu, David S. Strayer
Journal: Molecular Therapy (2005)

In situ gene transfer to hematopoietic stem/progenitor cells by intramarrow injection of viral vectors has been rarely reported, despite the potential promise of such an approach and the distribution of transgene-expressing cells in the body after intramarrow gene delivery has never been studied. We transduced the femoral bone marrow of rats by intramarrow injection of a Tag-deleted recombinant SV40 vector carrying a marker gene (FLAG epitope appended to HIV-1 Nef as a carrier protein). Control animals received a control rSV40 vector. Transgene expression in the blood endured throughout the 18 months of the study: 4-12% (average, 5%) of blood nucleated cells of all lineages were positive for FLAG through the end of the study. Circulating peripheral blood mononuclear cells and granulocytes expressed the transgene. The distribution of FLAG-positive cells was studied in different organs (lung, skeletal muscle, heart, kidney, liver, spleen, brain) at different times (4, and 18 months) after the injection of SV40 (Nef-FLAG) into the bone marrow. Very rare FLAG-positive cells were observed outside the muscle fibers in the skeletal muscles studied (TA, Soleus, EDL), as well as in the heart. FLAG-positive cells were seen in the kidney, liver, and spleen. Numerous FLAG-positive cells were seen in the lungs, more particularly in alveoli. By double labeling, it was found that these FLAG-positive cells were not epithelial. By double immunostaining, FLAG-positive cells in the alveoli mainly marked with CD68, which identifies macrophages. The percentages of FLAG-positive cells in alveoli were between 2.5 and 4 %, respectively 4 and 18 months after the injection of SV40 (Nef-FLAG) into the bone marrow cavity. No FLAG expression was seen in the control animals. Similar results were observed when using 3 different antibodies against FLAG. These results show that: 1) efficient gene transfer of hematopoietic stem/progenitor cells can be achieved by intramarrow injection of SV40 (Nef-FLAG); 2) differentiated derivatives of transduced bone marrow cells can migrate to different organs; 3) although rSV40 vectors can transduce many epithelial organs effectively, intramarrow injection of these vectors does not lead to appreciable transgene expression beyond blood forming cells and their derivatives.
The added value of quality of life (QoL) for prognosis of overall survival in patients with palliative hepatocellular carcinoma
Authors: Momar Diouf, Thomas Filleron, Jean-Claude Barbare, Loïc Fin, Carl Picard, Olivier Bouché, Laetitia Dahan, Xavier Paoletti, Franck Bonnetain
Journal: Journal of Hepatology (2012)
Abstract LB-208: Notch2 <i>in vivo</i> genetic labeling reveals novel epithelial subtypes within mammary luminal lineage
Authors: Sanja Sale, Daniel Lafkas, Spyros Artavanis-Tsakonas
Journal: Cancer Research (2012)

Abstract Identification and characterization of normal epithelial subtypes in mammary gland is a fundamental step in understanding mammary development as well as carcinogenesis. In this study we utilized an inducible lineage tracing approach in combination with reconstitution assays and conditional in vivo cell ablation to systematically map the fate and define cellular hierarchy of normal mammary epithelial cells (MECs) in postnatal mammary gland. We used a set of novel genetically engineered mice designed to follow Notch receptor expressing cell lineages given that Notch activity has been associated with early precursor and stem cells in many tissues. We focused our attention on Notch2 expressing cells, which allowed us to define two novel epithelial subtypes within ductal and alveolar lineage displaying distinct morphological and functional characteristics. We operationally term them as LumS (Luminal Small) and LumL (Luminal Large) cells. LumS cells are positioned in regular intervals alongside ductal longitudinal axis and are grouped in a chain formation around a single large luminal cell forming a stereotypical “pit”. LumL cells are large, oval shaped and appear as either singlets or doublets. They are mostly, although not exclusively, positioned in close proximity to branching points. In lactating glands LumL cells also appear as singlets or doublets within each alveolus. Functional assays demonstrate that LumS and LumL cells are not multipotent stem cells but rather differentiated luminal subtypes that are involved in determining location of the future tertiary alveolar structures as well as branching morphogenesis. It remains to be determined whether LumS and LumL populations are the cells of origin for breast cancer subtypes. We note with interest however that a single nucleotide polymorphism (SNP) in Notch2 locus has recently been identified as a novel genetic risk factor for breast cancer and Notch2 mRNA was found to be enriched in ER+ luminal tumors suggesting the possibility of clonal expansion of one or more Notch2+ lineages. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-208. doi:1538-7445.AM2012-LB-208
A Simple Test for Interatrial Communication
Authors: G. d. J. Lee, T. M. D. Gimlette
Journal: BMJ (1957)

Atrial septal defect -is one of the commoner congenital heart lesions.Wood (1956) found it in 18% of 900 cases of congenital heart disease that he studied.A large proportion of patients with this defect remain symptom-free for many years, but the average age of those seen by Wood was 23 years, and 84.5% were under 40, which suggests that these individuals tend to die young.Therefore it is not surprising that methods for surgical closure of the defect have now been developed, and, although the operative risks are being reduced, the
Angiogenic effect of intramuscular administration of basic and acidic fibroblast growth factor on skeletal muscles and influence of exercise on muscle angiogenesis * Commentary
Authors: A Efthimiadou, B Asimakopoulos, N Nikolettos, A Giatromanolaki, E Sivridis, D N Papachristou, E Kontoleon
Journal: British Journal of Sports Medicine (2005)

Angiogenic factors which control the angiogenic process represent a promising strategy for restoration of blood flow, but require further evaluation before clinical use. Exercise has also been reported to induce neovascularisation in muscles.To evaluate the angiogenic effects of basic fibroblast growth factor (b-FGF) and acidic fibroblast growth factor (a-FGF) on rat gastrocnemius muscle, when administered intramuscularly, and to compare them with those obtained by daily exercise.Forty nine rats were allotted to the following groups: A, controls; B, exercise by swimming; C1 and C2, intramuscular injection of b-FGF and a-FGF respectively; D1 and D2, b-FGF and a-FGF injection in combination with exercise. The antibody mouse anti-rat CD31 was used to evaluate the numbers of blood vessels present in histological preparations of gastrocnemius muscle.Significant increases in the numbers of blood vessels of the right gastrocnemius muscles in groups C1 and D1 were observed compared with controls (p<0.05). There was only a slight increase in angiogenesis in the left gastrocnemius muscle of groups C1 and D1 compared with controls (p>0.05), and there was a decrease in angiogenesis in the gastrocnemius muscle of the swimming group compared with controls.The intramuscular administration of b-FGF, but not a-FGF, induced significant local angiogenesis in gastrocnemius muscle at the site of injection.
Prof. Osler and the Assistant Surgeon in the Spanish American War.
Authors: Alexander Nettelroth
Journal: JAMA (1901)

Louisville, Ky., March 25, 1901. <h3>To the Editor:</h3> —In view of the many adverse criticisms of the diagnostic abilities of the surgeons in charge of the sick during the Spanish-American War, I consider it a duty to show that only an entire misapprehension of the facts could possibly excuse such severe and unjustifiable indictments as exemplified in the following quotation from Dr. Osler's paper on "Medicine in the Nineteenth Century." The quotation, which is only one of many similar in tone, is as follows: "The worst indictment ever brought against the medical schools of this country is contained in a report by Reed, Vaughan and Shakespeare on the prevalence of typhoid fever during the Spanish-American War. Shades of W. W. Gerhard and of Austin Flint! The young doctors to whom we intrusted scores of valuable lives, had practically not gotten beyond the nosology of Rush. Of the total number of
Studies of Pulmonary Function
Authors: B. G. Ferris
Journal: New England Journal of Medicine (1960)

IN clinical medicine before World War II, pulmonary-function studies, if done at all, were limited to measuring the vital capacity and possibly the maximal breathing capacity. The arterial oxygen saturation and the acid–base relations were also occasionally determined to estimate the adequacy of respiratory function. During World War II practical problems encountered in high-altitude flying, notably those associated with pressure breathing, led to an increased interest and more extensive investigations requiring the development of new technics and improvements in the existing methods. The usefulness of the new technics in clinical medicine was soon realized. More recently, pulmonary-function studies have been . . .
Regional myocardial blood flow and coronary vascular reverse in unanesthetized vascular reserve in unanesthetized young calves with severe concentric right ventricular hypertrophy.
Authors: M Manohar, J C Thurmon, W J Tranquilli, M D Devous, M C Theodorakis, R V Shawley, D L Feller, J G Benson
Journal: Circulation Research (1981)
Activation of Purinergic Receptors Triggers Oscillatory Contractions in Adult Rat Ventricular Myocytes
Authors: Bin-Xian Zhang, Xiuye Ma, Bradley K. McConnell, Derek S. Damron, Meredith Bond
Journal: Circulation Research (1996)

Extracellular ATP is an important neurotransmitter that modulates cardiac function by activation of purinergic receptors. In this study, the effect of P2 purinergic receptor activation on contractions and on [Ca2+]i was investigated in adult rat ventricular myocytes. Fura 2 was used to measure [Ca2+]i, and video edge detection was used to measure contraction. Superfusion of 2-methylthio-adenosine-5'-triphosphate (2-M-S-ATP) over quiescent myocytes induced oscillations in contraction and in [Ca2+]i. The frequency of the oscillatory contractions increased with increasing concentrations of 2-M-S-ATP, but the amplitude of contractions varied from cell to cell and was independent of the concentration of 2-M-S-ATP. During electrical stimulation, activation of purinergic receptors in myocytes potentiated the amplitude of contraction and induced arrhythmias. In populations of quiescent myocytes, the plateau phase of the [Ca2+]i signal evoked by 2-M-S-ATP could be shown to represent summed oscillations in [Ca2+]i in individual cells. Pretreatment of quiescent myocytes with thapsigargin or caffeine reduced or abolished the oscillations in contractions and in [Ca2+]i triggered by 2-M-S-ATP, indicating a dependence of the oscillations on uptake and release of Ca2+ by the sarcoplasmic reticulum. These data demonstrate the novel phenomenon that activation of purinergic receptors in quiescent myocytes stimulates oscillations in [Ca2+]i and contraction. In electrically stimulated myocytes, activation of purinergic receptors triggers oscillatory contractions and potentiates the amplitude of electrically triggered contractions.
P4-18-03: Basal-Like Phenotype of BRCA Associated Ductal Carcinoma In-Situ.
Authors: K Kristjansdottir, J Laprise, J Scalia-Wilbur, R Simon, M Steinhoff, R Legare
Journal: Cancer Research (2011)

Abstract Background: Molecular profiling has identified different subtypes of invasive carcinoma. The basal-like tumors represent a subgroup that is known for aggressive behavior. The NCCN guidelines recommend genetic testing for patients with triple negative breast cancer as over 80% of breast cancers in BRCA1 mutation carriers are triple negative tumors, and 70% of those fulfill criteria for basal-like tumors. Ductal carcinoma in-situ (DCIS) is thought to represent a non-obligate precursor to invasive breast cancer. Little is known about basal-like precursor lesions and their association with germline BRCA mutations. The aims of this study were to characterize the immunohistochemical phenotype of BRCA associated DCIS and evaluate the clinical importance of such lesions. Material and Methods: We identified 32 cases of DCIS from 28 women with a known BRCA1/2 mutation, from our genetics database. Tumor samples were reviewed to identify areas of DCIS for staining. Five micron sections were stained on the Dako Autostainer with antibodies to estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER-2), epidermal growth factor receptor (EGFR), and CK 5/6. Results: 44% of the DCIS tumors were from BRCA1 mutation carriers and 56% from BRCA2 mutation carriers. 53% of the samples were triple negative (staining negative for ER, PR, HER-2) and 69% of the triple negative tumors were basal-like tumors (staining positive for either EGFR or CK5/6). The basal-like DCIS specimens were all high grade, and they all came from BRCA1 positive patients. 71% of BRCA1 positive DCIS had basal-like features. Conclusions: DCIS in BRCA1 mutation carriers shows basal-like features that are in line with historical control for invasive carcinomas in the same group, suggesting that DCIS is a precursor lesion for BRCA1 positive cancer. Although further investigation is needed, our data suggest that patients with basal-like DCIS should be offered genetic testing. Citation Information: Cancer Res 2011;71(24 Suppl):Abstract nr P4-18-03.
An alpha 2-macroglobulin receptor-dependent mechanism for the plasma clearance of transforming growth factor-beta 1 in mice.
Authors: J LaMarre, M A Hayes, G K Wollenberg, I Hussaini, S W Hall, S L Gonias
Journal: Journal of Clinical Investigation (1991)

Radioiodinated transforming growth factor-beta 1 (TGF-beta 1) bound to the plasma proteinase inhibitor, alpha 2-macroglobulin (alpha 2M), as determined by chromatography on Superose-6 and native polyacrylamide gel electrophoresis. When alpha 2M conformational change was induced with methylamine, 125I-TGF-beta 1 binding significantly increased. Intravenously injected 125I-TGF-beta 1 cleared from the circulation of mice rapidly at first; however, intravascular radioactivity stabilized near 20% of the initial level. At necropsy, radioactivity was recovered predominantly in the liver (65%); however, the density of radioactivity (disintegrations per minute/g organ wt) was highest in the lungs. Markedly different results were obtained with purified 125I-TGF-beta 1-alpha 2M-methylamine complex. Clearance of the complex occurred as a first-order process with a t1/2 of 4 min. Greater than 90% of the radioactivity was recovered in the liver. The clearance and distribution of 125I-TGF-beta 1-alpha 2M-methylamine were equivalent to those observed with 125I-alpha 2M-methylamine and 125I-alpha 2M-trypsin. The latter two radioligands clear via specific alpha 2M receptors in the liver. Large molar excesses of alpha 2M-trypsin or alpha 2M-methylamine competed with 125I-TGF-beta 1-alpha 2M-methylamine for plasma clearance. Native alpha 2M, which does not bind to the alpha 2M receptor, did not compete. The receptor binding domain of alpha 2M-methylamine was blocked by chemical modification or enzyme treatment. The resulting alpha 2M preparations still bound 125I-TGF-beta 1; however, the complexes did not clear when injected intravenously in mice. The studies presented here demonstrate that alpha 2M can mediate the plasma clearance of a growth factor via the alpha 2M receptor system. We propose that alpha 2M, the alpha 2M receptor, and proteinases may function as a concerted system to regulate TGF-beta 1 activity and the activity of related factors in vivo.