tbuckley/cabot-search huggingface dataset:
Light‐Mediated Activation of siRNA Release in Diblock Copolymer Assemblies for Controlled Gene Silencing
Authors: Abbygail A. Foster, Chad T. Greco, Matthew D. Green, Thomas H. Epps, Millicent O. Sullivan
Journal: Advanced Healthcare Materials (2014)

Controllable release is particularly important for the delivery of small interfering RNA (siRNA), as siRNAs have a high susceptibility to enzymatic degradation if release is premature, yet lack silencing activity if they remain inaccessible within the cytoplasm. To overcome these hurdles, novel and tailorable mPEG‐ b ‐poly(5‐(3‐(amino)propoxy)‐2‐nitrobenzyl methacrylate) (mPEG‐ b ‐P(APNBMA)) diblock copolymers containing light‐sensitive o ‐nitrobenzyl moieties and pendant amines are employed to provide both efficient siRNA binding, via electrostatic and hydrophobic interactions, as well as triggered charge reversal and nucleic acid release. In particular, siRNA/mPEG‐ b ‐P(APNBMA) 23.6 polyplexes show minimal aggregation in physiological salt and serum, and enhanced resistance to polyanion‐induced unpackaging compared to polyethylenimine preparations. Cellular delivery of siRNA/mPEG‐ b ‐P(APNBMA) 23.6 polyplexes reveals greater than 80% cellular transfection, as well as rapid and widespread cytoplasmic distribution. Additionally, UV irradiation indicates ≈70% reduction in targeted gene expression following siRNA/mPEG‐ b ‐P(APNBMA) 23.6 polyplex treatment, as compared to 0% reduction in polyplex‐treated cells without UV irradiation, and only ≈30% reduction for Lipofectamine‐treated cells. The results here highlight the potential of these light‐sensitive copolymers with a well‐defined on/off switch for applications including cellular patterning for guided cell growth and extension, and cellular microarrays for exploring protein and drug interactions that require enhanced spatiotemporal control of gene activation.
THE WORK AND PAY OF HEALTH OFFICERS.Published by permission of the Michigan State Medical Society.
Authors: HENRY B. BAKER
Journal: JAMA (1892)

<i>Mr. President and Members of the State Medical Society:</i>—I wish, first of all, to thank the Section of Practice of Medicine for this opportunity, to place before you what I have to offer. I have assumed that I would be expected to deal with some subject likely to be of interest to the general practitioners, and which my own occupation might enable me to have more than ordinary opportunities for studying. Inasmuch as the State law now requires that, whereever it is practicable, every health officer shall be a physician, and there are now fifteen hundred health officers chosen in Michigan every year, there is a possibility of my subject being of interest to a considerable proportion of the general practitioners in the State, because many of them may, at some time, be health officers, and because, if my views were to be carried out, nearly fifteen hundred practitioners would
Doughnut Phenomenon in Bone Scintigraphy
Authors: DOV FRONT, RUTH HARDOFF
Journal: Clinical Nuclear Medicine (1978)

A doughnut phenomenon was observed in bone scintigraphy in three cases of metastatic thyroid carcinoma to the skull. Additional dynamic and static studies were performed to evaluate the relative roles of vascularity, vessel permeability and new bone formation in producing this phenomenon. This was accomplished through the use of 99Tc-diphosphonate, 99Tc-labeled red blood cells and 99Tc-pertechnetate. The important factor was found to be a new bone formation. Late uptake of diphosphonate was shown only in the periphery of the lesion, where formation of new bone takes place. This occurred even in the presence of increased vascularity and vessel permeability in the lesion as a whole.
INCIDENCE OF LEAD POISONING IN THE CITY OF BALTIMORE
Authors: JOHN M. McDONALD
Journal: JAMA (1942)

Since 1935 the Bureau of Laboratories of the Baltimore City Health Department has provided a routine laboratory service for the quantitative estimation of lead in blood and other body fluids as an index of abnormal lead absorption in cases of suspected lead poisoning.<sup>1</sup>The cooperation of those physicians and hospitals who made use of this service was solicited in order to make available the clinical histories of the persons whose bloods were examined for lead. In this way considerable information was obtained about the local occurrence of both industrial and nonindustrial lead poisoning. A summary of the incidence of lead poisoning in the city of Baltimore during the period 1931-1940 is shown in table 1. These data were obtained from the followingsources: (1) reports of occupational lead poisoning, (2) reports of deaths from lead poisoning and (3) cases ascertained in a follow-up of the blood lead laboratory
Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged antigens: Preparation, structural study and immune response towards rHsp90
Authors: Josef Mašek, Eliška Bartheldyová, Pavlína Turánek-Knotigová, Michaela Škrabalová, Zina Korvasová, Jana Plocková, Štěpán Koudelka, Petra Škodová, Pavel Kulich, Michal Křupka, Kateřina Zachová, Lýdie Czerneková, Milada Horynová, Irena Kratochvílová, Andrew D. Miller, Daniel Zýka, Jaroslav Michálek, Jana Vrbková, Marek Šebela, Miroslav Ledvina, Milan Raška, Jaroslav Turánek
Journal: Journal of Controlled Release (2011)
Intractable heart failure, conduction disturbances and myocardial infarction by massive myocardial invasion of malignant lymphoma
Authors: Toshihisa Miyazaki, Teruhiko Yoshida, Hidezo Mori, Kazuto Yamazaki, Shunnosuke Handa, Yoshiro Nakamura
Journal: Journal of the American College of Cardiology (1985)
Housing in London
Authors: S. Carne
Journal: BMJ (1961)

About a year ago I was consulted by a patient who suffers from psoriasis.He is employed as a carpenter by a teaching hospital (not in this area of London), and he had been attending the dermatology out-patient department there for some time.At his last attendance he had been given treatment which involved a daily bath-but he had no bath at his home.I was not surprised that this was so, for I knew tha-t bathrooms were a relative luxury in Shepherd's Bush; but when I mentioned this story to a friend at hospital he seemed surprised at the lack of what are regarded as essential amenities.I therefore thought it would be worth while making a more detailed analysis of the living conditions of my patients.My practice is mainly in Shepherd's Bush, part of the Metropolitan Borough of Hammersmith in the west of London.Local landmarks are the B.B.
Hominoid chromosomal rearrangements on 17q map to complex regions of segmental duplication
Authors: Maria Francesca Cardone, Zhaoshi Jiang, Pietro D'Addabbo, Nicoletta Archidiacono, Mariano Rocchi, Evan E Eichler, Mario Ventura
Journal: Genome biology (2008)

Chromosomal rearrangements, such as translocations and inversions, are recurrent phenomena during evolution, and both of them are involved in reproductive isolation and speciation. To better understand the molecular basis of chromosome rearrangements and their part in karyotype evolution, we have investigated the history of human chromosome 17 by comparative fluorescence in situ hybridization (FISH) and sequence analysis.Human bacterial artificial chromosome/p1 artificial chromosome probes spanning the length of chromosome 17 were used in FISH experiments on great apes, Old World monkeys and New World monkeys to study the evolutionary history of this chromosome. We observed that the macaque marker order represents the ancestral organization. Human, chimpanzee and gorilla homologous chromosomes differ by a paracentric inversion that occurred specifically in the Homo sapiens/Pan troglodytes/Gorilla gorilla ancestor. Detailed analyses of the paracentric inversion revealed that the breakpoints mapped to two regions syntenic to human 17q12/21 and 17q23, both rich in segmental duplications.Sequence analyses of the human and macaque organization suggest that the duplication events occurred in the catarrhine ancestor with the duplication blocks continuing to duplicate or undergo gene conversion during evolution of the hominoid lineage. We propose that the presence of these duplicons has mediated the inversion in the H. sapiens/P. troglodytes/G. gorilla ancestor. Recently, the same duplication blocks have been shown to be polymorphic in the human population and to be involved in triggering microdeletion and duplication in human. These results further support a model where genomic architecture has a direct role in both rearrangement involved in karyotype evolution and genomic instability in human.
Infektionskrankheiten, Vol II: Krankheiten durch Bakterien
Authors: Norman Roberg
Journal: JAMA (1970)

<b>Die Infektionskrankheiten des Menschen und ihre Erreger,</b>by Arthur Grumbach and Otto Bonin, 2 vol, ed 2; 1,958 pp, 172 illus., $92.50, 370 marks, Stuttgart, Georg Thieme Verlag (New York, Intercontinental Medical Book Corp), 1969. Gsell and Mohr present two excellent volumes on bacterial disease. These volumes, the second of a three-part series on infectious disease, belong to a new edition of<i>Handbuch der Inneren Medizin</i>. Like the first two volumes on viral disease already reviewed (<i>JAMA</i><b>102:</b>169, Dec 4, 1967), each chapter is a monograph dealing with a group of organisms and the diseases which they cause. Authorship is international. The organization, printing, and format are consistent and excellent, and the monographs are suited for quick reference as well as for thorough and enjoyable reading. The sections on microbiology, immunology, prophylaxis, epidemiology, and therapy are balanced, but with a wholesome emphasis upon clinical course and complications. The many
The zinc-finger protein MAZR is part of the transcription factor network that controls the CD4 versus CD8 lineage fate of double-positive thymocytes
Authors: Shinya Sakaguchi, Matthias Hombauer, Ivan Bilic, Yoshinori Naoe, Alexandra Schebesta, Ichiro Taniuchi, Wilfried Ellmeier
Journal: Nature Immunology (2010)
Incidence and predictors of severe extra-articular disease manifestations in an early rheumatoid arthritis inception cohort
Authors: C Turesson, K Eberhardt, L T H Jacobsson, E Lindqvist
Journal: Annals of the Rheumatic Diseases (2007)

Neuroinflammation is an important contributor to Alzheimer9s disease (AD) pathogenesis, as underscored by the recent identification of immune-related genetic risk factors for AD, including coding variants in the gene <i>triggering receptor expressed on myeloid cells 2 (TREM2</i>). Understanding TREM2 function promises to provide important insights into how neuroinflammation contributes to AD pathology. However, studies so far have produced seemingly conflicting results, with reports that amyloid pathology can be both decreased and increased in TREM2 deficient AD mouse models. In this study, we unify these previous findings by demonstrating that TREM2 deficiency ameliorates amyloid pathology early, but exacerbates it late in disease progression in the APPPS1-21 mouse model of AD. We also demonstrate that TREM2 deficiency decreases plaque-associated myeloid cell accumulation by reducing cell proliferation, specifically late in pathology. In addition, TREM2 deficiency reduces myeloid cell internalization of amyloid throughout pathology, but selectively decreases inflammation-related gene transcript levels late in disease progression. Together, these results suggest that TREM2 plays distinct functional roles at different stages in AD pathology. <b>SIGNIFICANCE STATEMENT</b> Alzheimer9s disease (AD) is a devastating neurodegenerative disorder, and there are currently no effective treatments which modify disease progression. However, the recent identification of genetic risk factors for AD promise to provide new insight into AD biology and possible new therapeutic targets. Among these risk factors, variants in the gene <i>TREM2</i> confer greatly elevated risk for developing the disease. We demonstrate that TREM2 deficiency has opposing effects on AD-related pathologies at early and late stages of disease progression, unifying previous work in the field. In addition, we examine how TREM2 effects the function of the brain immune cell populations in which it9s expressed throughout disease progression to understand possible mechanisms underlying its differential impacts on pathology.
Freedom, fallibility, and the energy of motor vehicles
Authors: J. Francis
Journal: BMJ (1981)

is not simply an exercise effect is indicated by similar responses at rest in the laboratory setting.Although the laboratory procedures were not presented in a random order they were interrupted by rest periods, particularly before the discotheque when refreshments were served.We do not claim to provide a definitive basis for advice to epileptic children regarding the safety of their attending discotheques, but rather question the tendency to advise them to avoid discotheques.Furthermore, we suggest that some laboratory procedures might be helpful in advising an individual patient.
Manual of Childhood Infections
Authors: D. Isaacs
Journal: BMJ (1996)

E Graham Davies, David A C Elliman, C Anthony Hart, Angus Nicoll, Peter T Rudd Saunders, pounds sterling17.95, pp 393 ISBN 0 7020 1832 5

“The only book written by a committee that is beyond criticism is the King James Version of the Bible.” Thus Stan Plotkin in his introduction to the red book. The red book is for many of us the Bible of paediatric infectious diseases, produced as a Report of the Committee on Infectious Diseases for the American Academy of Pediatrics, and revised every two to three years. We may not agree with all the recommendations, but the scientific basis behind them is likely to …
Purple Urine Bag Syndrome
Authors: Taro Kojima
Journal: JAMA (2012)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Oxidative stress in asymptomatic subjects with hereditary hemochromatosis
Authors: Yukitaka Shizukuda, Charles D. Bolan, Tammy T. Nguyen, Gilberto Botello, Dorothy J. Tripodi, Yu‐Ying Yau, Myron A. Waclawiw, Susan F. Leitman, Douglas R. Rosing
Journal: American Journal of Hematology (2006)
Primary Type V Hyperlipoproteinemia
Authors: BARRY H. GREENBERG
Journal: Annals of Internal Medicine (1977)

We have studied 32 kindreds identified by propositi with primary type V hyperlipoproteinemia. The clinical presentation, metabolic associations, and natural history confirm the distinctiveness of primary type V hyperlipoproteinemia from other lipoprotein abnormalities. Although the underlying defect(s) remains unknown, several factors such as obesity, alcohol, drugs, and diet are able to modify the glyceridemia, the major manifestation of this disorder. Abnormalities of postheparin lipolytic activity or its subfractions do not appear to be involved in the pathogenesis of primary type V. The prevalence of hyperuricemia, diabetes, pancreatitis, and xanthomatosis appears high among the 32 propositi; the last two entities are much less prevalent in the relatives, even among those relatives classified as hyperglyceridemic. There is no evidence in these families of excessive coronary artery disease prevalence. Triglyceride levels are positively associated with age in this population, especially among women. Average triglyceride levels were lower for women than for men before age 50.
Otosclerosis associated with osteopoikilosis
Authors: J. M. Strosberg
Journal: JAMA (1981)

<h3>To the Editor.—</h3> The letter by Jack R. Harnes, MD (1981;245:342), entitled "Seventy Percent Physicians" sets up an untenable dichotomy of competence vs incompetence. Attempts to define physician competence show its complexity. We usually deal with varying degrees of competence dependent on the patient, the illness, and the context in which the interaction occurs. Neither licensure nor certification attests to life-long, unvarying competence of an absolute nature. These credentials should provide some assurance to patients of an expectable degree of relevant knowledge, skills, and judgment, essential for the safe practice of medicine. By no means can we rightfully expect perfection. The letter further confuses the issue by not recognizing the function of a written, objective, multiplechoice type of examination. No one has ever demonstrated a near-perfect degree of correlation between scores on such examinations and actual clinical performance. However, poor grades that reflect a pervasive lack of knowledge across the
SOME INTERESTING AUTOPSY FINDINGS IN EPILEPTICS.
Authors: B. ONUF (ONUFROWICZ)
Journal: JAMA (1905)

When I assumed the duties of pathologist to the Craig Colony, a year ago, the task before me of studying the pathology of epilepsy seemed a hard one, promising but little fruit. That such a pessimistic feeling had justification will be seen from the following passage by an author of such repute as Gowers,<sup>1</sup>and whose name is so intimately associated with the subject of epilepsy: Of the histologie changes revealed by the microscope, most of those which are not common, apart from symptoms, are apparently of secondary origin, the result and not the cause of the convulsions; and the various changes that have been described and have been supposed to be related to the origin of the disease have probably no connection with it, and do not even deserve enumeration. No greater significance can be ascribed to the induration of the cornua ammonis (pes hippocampi) to which weight has been attached by Meynert.
Hip-Joint Disease and Its Early Treatment
Authors: J. Croft
Journal: BMJ (1885)

FmsrsT I woul(d deline the class of hip-disease to which my observa- tions particularly apply.I (lassify hip-joint disease primarily under two heads, acute and chronic.WVe meet with acute synovitis and acute arthritis ; acute syneovitis of simple ser-ous catarrlsal character; acute suppurative synov-itis; and the infective suppurative inflamma- tions, as the gonorrlical and pys,emic.I do not refer to any of these.We meet with acuite arthritis when other articular structures beyond the synovial menmbrane are afrected.This acute variety commonly ends in acute necrosis of the epiphysis of the femur, or portion of the acetabuilum; it is sometimes designated acute epiphysitis.I dlo nlot refer specially to this.Mly observations are linsited to the chronic variety of arthritis, and witlh respect to this I shall confine mnyself to parts of the subject.First, with respeit to the st-umous, orscrofulous, or tuberculai- variety or forms of the disease, I am quite convinced, by examiination of parts removed from-i mlly own cases of excision, now amounting to sixty-eight in number, thalt clhronic hip-joint disease in cllildren is met with in thlree several kinlds: 1, siInple * 2, strumous or scrofu-.ons,which are synionymous ; 3, tuberculous.Each kind may coIi- tinuc distinct from its commencement to its termination, but the second and third may be the sources of other or general tuberculosis.
Sex Differences in Career Development Awardees' Subsequent Grant Attainment
Authors: Reshma Jagsi, Peter A. Ubel
Journal: Annals of Internal Medicine (2010)

Letters4 May 2010Sex Differences in Career Development Awardees' Subsequent Grant AttainmentReshma Jagsi, MD, DPhil and Peter A. Ubel, MDReshma Jagsi, MD, DPhilFrom University of Michigan, Ann Arbor, MI 48109.Search for more papers by this author and Peter A. Ubel, MDFrom University of Michigan, Ann Arbor, MI 48109.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-152-9-201005040-00020 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail IN RESPONSE:Dr. Pohlhaus and colleagues have provided useful data supporting our identification of a leakage problem, especially for women, in the academic pipeline. However, we wish to clarify a few points.First, there is no reason to expect that female K awardees have less research desire or talent than male peers; thus, the lower rate of R01 applications among women is a cause for concern. We need to understand why a lower proportion of promising women reach the point of applying for an R01 award.Second, the rates of R01 attainment we presented were calculated through actuarial analysis, censored ... Author, Article, and Disclosure InformationAffiliations: From University of Michigan, Ann Arbor, MI 48109.Disclosures: None disclosed. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoSex Differences in Attainment of Independent Funding by Career Development Awardees Reshma Jagsi , Amy R. Motomura , Kent A. Griffith , Soumya Rangarajan , and Peter A. Ubel Sex Differences in Career Development Awardees' Subsequent Grant Attainment Jennifer Reineke Pohlhaus , Hong Jiang , and Jennifer Sutton Metrics Cited ByAssessing Gender Equity in a Large Academic Department of Pediatrics 4 May 2010Volume 152, Issue 9Page: 617KeywordsCareers in researchConflicts of interest ePublished: 4 May 2010 Issue Published: 4 May 2010 CopyrightCopyright © 2010 by American College of Physicians. All Rights Reserved.PDF DownloadLoading ...
Medical Futility: Response to Critiques
Authors: Lawrence J. Schneiderman
Journal: Annals of Internal Medicine (1996)

Six years ago, we proposed a patient benefit-centered definition of medical futility that included both quantitative and qualitative components. We distinguished between an effect of a treatment that is limited to some part of a patient's body and a benefit that improves the patient as a whole. The quantitative portion of our definition stipulated that physicians should regard a treatment as futile if empirical data show that the treatment has less than a 1 in 100 chance of benefiting the patient. The qualitative portion of our definition stipulated that if a treatment merely preserves permanent unconsciousness or cannot end dependence on intensive medical care, physicians should consider the treatment futile. In this paper, we clarify and modify our original proposal and respond to the following major criticisms: 1) Medical futility is simply an attempt to increase the power of the physician over the patient and to repeal recent hard-gained advances in patient autonomy; 2) no professional or societal consensus has been achieved about the definition of futility; 3) futility is a value-laden determination, the usurpation of which by medicine is inappropriate unless only a so-called value-free or strict physiologic definition of futility is used; 4) the concept of futility is not practically useful because empirical treatment data cannot be applied with certainty to any given patient; 5) futility undermines our pluralistic society and threatens, among other things, the free exercise of religion; and 6) because cost considerations will ultimately dictate all such decisions, futility is an unnecessary concept.