tbuckley/cabot-search huggingface dataset:
Rapid diagnosis in varicella and herpes zoster: re-evaluation of direct smear (Tzanck test) and electron microscopy including colloidal gold immuno-electron microscopy in comparison with virus isolation
Authors: E. FOLKERS, J. VREESWIJK, A.P. ORANJE, J.N. DUIVENVOORDEN
Journal: British Journal of Dermatology (1989)

Journal Article Rapid diagnosis in varicella and herpes zoster: re‐evaluation of direct smear (Tzanck test) and electron microscopy including colloidal gold immuno‐electron microscopy in comparison with virus isolation Get access E. FOLKERS, E. FOLKERS Department of Dermatology, Hospital 'de Heel', Zaandam, The Netherlands Dr E.Folkers, Department of Dermatology, Hospital 'de Heel', Postbox 210, NL‐1500 EE Zaandam, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar J. VREESWIJK, J. VREESWIJK Department of Virology, Laboratory of Electron Microscopy, Central Veteinary Institute, Lelystad, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar A.P. ORANJE, A.P. ORANJE Department of Dermatology and Venereology (Pediatric Dermatology), Erasmus University Rotterdam and University Hospital Rotterdam/Sophia Children's Hospital, Rotterdam, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar J.N. DUIVENVOORDEN J.N. DUIVENVOORDEN Department of Pathological Anatomy (Cytology), Hospital 'de Heel', Zaandam, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 121, Issue 3, 1 September 1989, Pages 287–296, https://doi.org/10.1111/j.1365-2133.1989.tb01421.x Published: 01 September 1989
The B cell antigen CD75 is a cell surface sialytransferase.
Authors: I Stamenkovic, H C Asheim, A Deggerdal, H K Blomhoff, E B Smeland, S Funderud
Journal: The Journal of Experimental Medicine (1990)

In this work we have isolated a cDNA clone encoding the B cell antigen CD75. The amino acid sequence of CD75 is shown to be identical to that of human alpha 2,6 sialyltransferase, believed to be primarily associated with the Golgi complex. This is the first demonstration of cell surface expression of sialytransferase which, in B cells, may play an important role in intercellular adhesion and antigen presentation events.
Reestablishment of the Endothelial Lining by Endothelial Cell Therapy Stabilizes Experimental Abdominal Aortic Aneurysms
Authors: Grégory Franck, Jianping Dai, Alexandre Fifre, Saravuth Ngo, Claire Justine, Stéphanie Michineau, Eric Allaire, Marianne Gervais
Journal: Circulation (2013)

Background— Loss of the endothelium and its replacement by a thick thrombus are structural features of human abdominal aortic aneurysms (AAAs). In AAAs, the relationship between aortic diameter expansion, the presence of thrombus, and the lack of endothelial cells (ECs) remains unexplored. We hypothesized that reendothelialization by cell therapy would modulate aortic wall destruction and ultimately stabilize AAAs. We evaluated the impact of local seeding of rat aortic ECs or peripheral blood–derived outgrowth ECs on AAA evolution. Methods and Results— Rat aortic ECs (n=30) or serum-free medium (controls; n=29) were seeded endovascularly immediately (day 0) or 14 days after surgery in the rat xenograft model. Rat aortic EC seeding prevented AAA formation and stabilized formed AAAs at 28 days (diameter increase at day 0+28, 51±6% versus 83±6%; day 14+28, −1±4% versus 22±6% in rat aortic ECs and controls, respectively; P &lt;0.01). This stabilizing effect was associated with the reestablishment of the endothelial lining, the suspension of proteolysis, and the reconstitution of new aortic wall rich in smooth muscle cells and extracellular matrix. Transplanted rat aortic ECs did not participate directly in aortic wall repair but exerted their healing properties through paracrine mechanisms involving the upregulation of endothelium-derived stabilizing factors and the recruitment of resident vascular cells. In rats, the transplantation of outgrowth ECs (n=7) significantly reduced by 30% the progression of AAAs and restored the abluminal endothelium at 28 days compared with controls (n=9). Conclusion— Our study demonstrates the potential of restoring the endothelial lining to control AAA dynamics and designates ECs as an efficient therapy to stop AAA expansion.
Impact of Chronic Obstructive Pulmonary Disease on the Mortality of Patients with Non–Small-Cell Lung Cancer
Authors: Seung Jun Lee, Jinwoo Lee, Young Sik Park, Chang-Hoon Lee, Sang-Min Lee, Jae-Joon Yim, Chul-Gyu Yoo, Sung Koo Han, Young Whan Kim
Journal: Journal of Thoracic Oncology (2014)
Abstract LB-368: Exploiting the mechanism of intron-splicing to produce viral vectors harboring toxin genes for cancer therapy
Authors: Haifeng Chen
Journal: Cancer Research (2012)

Abstract Suicide gene therapy has been widely investigated for the treatment of human immunodeficiency virus (HIV) infection, for controlling graft-versus-host disease, and also for the treatment of cancer. While the production of viral vectors carrying suicide genes such as the herpes simplex virus thymidine kinase (HSV-TK) or cytosine deaminase are easily achievable, the generation of viral vectors containing toxin genes such as diphtheria toxin A fragment (DT-A), Pseudomonas exotoxin (PE), and barnase has always been a challenge very difficult to fulfill due to the extreme toxicity of these toxins. We developed a novel way to produce in insect cells baculoviral and adeno-associated viral (AAV) vectors harboring toxin genes such as DT-A or Barnase by exploiting the different intron-splicing mechanisms between insect and mammalian cells. Since most of the mammalian introns are not functional in insect cells, we cloned two mammalian introns to interrupt the DT-A or Barnase coding sequences and produced recombinant baculoviral and AAV vectors at the normal level as compared with the baculoviral or AAV vectors carrying just the green fluorescent protein (GFP) gene. After transduction of the toxin gene-harboring baculoviral or AAV vectors into mammalian HEK293 cells, the mammalian introns were spliced out and toxins expressed and killed the HEK293 cells. This is the first report so far as we know that the differences in intron-splicing between insect and mammalian cells can be utilized to manufacture viral vectors harboring toxin genes. Our finding indicates that this novel method provides a valuable new tool for the development of toxin-based suicide gene cancer therapy. Bac, baculovirus; AAV, adenoassociated virus; inDTA (hGH), DT-A gene interrupted by human growth hormone intron; inBar (SV40)-GFP, Barnase gene interrupted by SV40 LT antigen intron and fused in-frame with GFP; vg, vector genome copy number; pfu, plaque-forming unit; ND, not done. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-368. doi:1538-7445.AM2012-LB-368
Abstract B18: MiR-205 puts the brakes on the malignant interplay between prostate cancer cells and associated fibroblasts
Authors: Paolo Gandellini, Elisa Giannoni, Anna Casamichele, Maria Letizia Taddei, Maurizio Callari, Riccardo Valdagni, Nadia Zaffaroni, Paola Chiarugi
Journal: Cancer Research (2013)

Abstract Tumor microenvironment is a strong determinant for the acquisition of metastatic potential of cancer cells. We have recently demonstrated that cancer associated fibroblasts (CAFs) elicit an epithelial-mesenchymal transition (EMT) in prostate cancer (PCa) cells, leading to enhanced tumor cell aggressiveness. Here, we investigated the involvement of microRNAs (miRNA) in such malignant tumor-stroma interplay, to identify possible tools to prevent metastasis dissemination. We found that miR-205 is the most down-modulated miRNA in PCa cells upon CAF stimulation and acknowledged HIF-1 to be a direct repressor of miRNA transcription. Rescue experiments demonstrated that ectopic miR-205 over-expression in PCa cells counteracts CAF-induced EMT, thus impairing enhancement of cell invasion, acquisition of stem cell traits, tumorigenicity and metastatic dissemination. In addition, miR-205 can block tumor-driven activation of surrounding fibroblasts, by reducing pro-inflammatory cytokine secretion. Overall, these findings suggest a key role of miR-205 in the bidirectional interplay between PCa cells and fibroblasts. In addition, the evidence that miR-205 replacement in PCa cells is able not only to prevent but also to revert EMT induced by CAFs sets up the rationale for developing miRNA-based approaches for the prevention and treatment of aggressive tumors. Citation Format: Paolo Gandellini, Elisa Giannoni, Anna Casamichele, Maria Letizia Taddei, Maurizio Callari, Riccardo Valdagni, Nadia Zaffaroni, Paola Chiarugi. MiR-205 puts the brakes on the malignant interplay between prostate cancer cells and associated fibroblasts. [abstract]. In: Proceedings of the AACR Special Conference on Tumor Invasion and Metastasis; Jan 20-23, 2013; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2013;73(3 Suppl):Abstract nr B18.
Approaching Hysteria: Disease and its Interpretations
Authors: S. Wessely
Journal: BMJ (1995)

Mark S Micale Princeton University Press, pounds sterling24.95, pp 327 ISBN 0 691 03717 5

For some medical specialties historical inquiry might seem to have little to offer, conjuring up images of elderly consultants writing the history of their hospitals. Not so in psychiatry, and not so in Mark Micale's enthralling account of the history of one particular disease—hysteria.

Micale, a professional historian, takes as his task not the illness itself but the way in which authors past and present have approached it. He uses hysteria to illuminate the history of ideas, tracing it through its diverse appearances in the psychiatric, neurological, literary, feminist, psychoanalytic, sociological, and historical literatures. He pays particular attention to French publications, for hysteria has always been the great neurosis of not just French medicine, but French art, science, literature, and culture. French society has conducted a longstanding and continuing love affair with hysteria. Guy de Maupassant said that “we are all hysterics.” Jules Clarette wrote that “the illness …
Whole-cell SELEX aptamer-functionalised poly(ethyleneglycol)-poly(ε-caprolactone) nanoparticles for enhanced targeted glioblastoma therapy
Authors: Huile Gao, Jun Qian, Zhi Yang, Zhiqing Pang, Zhangjie Xi, Shijie Cao, Yuchen Wang, Shuaiqi Pan, Shuang Zhang, Wei Wang, Xinguo Jiang, Qizhi Zhang
Journal: Biomaterials (2012)
Exacerbation of Chronic Renovascular Hypertension and Acute Renal Failure in Heme Oxygenase-1–Deficient Mice
Authors: Philippe Wiesel, Anand P. Patel, Irvith M. Carvajal, Zhi Yuan Wang, Andrea Pellacani, Koji Maemura, Nicole DiFonzo, Helmut G. Rennke, Matthew D. Layne, Shaw-Fang Yet, Mu-En Lee, Mark A. Perrella
Journal: Circulation Research (2001)

Heme oxygenase (HO) is a cytoprotective enzyme that degrades heme (a potent oxidant) to generate carbon monoxide (a vasodilatory gas that has anti-inflammatory properties), bilirubin (an antioxidant derived from biliverdin), and iron (sequestered by ferritin). Because of properties of HO and its products, we hypothesized that HO would be important for the regulation of blood pressure and ischemic injury. We studied chronic renovascular hypertension in mice deficient in the inducible isoform of HO (HO-1) using a one kidney-one clip (1K1C) model of disease. Systolic blood pressure was not different between wild-type (HO-1(+/+)), heterozygous (HO-1(+/-)), and homozygous null (HO-1(-/-)) mice at baseline. After 1K1C surgery, HO-1(+/+) mice developed hypertension (140+/-2 mm Hg) and cardiac hypertrophy (cardiac weight index of 5.0+/-0.2 mg/g) compared with sham-operated HO-1(+/+) mice (108+/-5 mm Hg and 4.1+/-0.1 mg/g, respectively). However, 1K1C produced more severe hypertension (164+/-2 mm Hg) and cardiac hypertrophy (6.9+/-0.6 mg/g) in HO-1(-/-) mice. HO-1(-/-) mice also experienced a high rate of death (56%) within 72 hours after 1K1C surgery compared with HO-1(+/+) (25%) and HO-1(+/-) (28%) mice. Assessment of renal function showed a significantly higher plasma creatinine in HO-1(-/-) mice compared with HO-1(+/-) mice. Histological analysis of kidneys from 1K1C HO-1(-/-) mice revealed extensive ischemic injury at the corticomedullary junction, whereas kidneys from sham HO-1(-/-) and 1K1C HO-1(+/-) mice appeared normal. Taken together, these data suggest that chronic deficiency of HO-1 does not alter basal blood pressure; however, in the 1K1C model an absence of HO-1 leads to more severe renovascular hypertension and cardiac hypertrophy. Moreover, renal artery clipping leads to an acute increase in ischemic damage and death in the absence of HO-1.
Learning to Hear
Authors: Neil Scheurich
Journal: JAMA (1992)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Mechanism, Experiment, Disease: Marcello Malpighi and Seventeenth-Century Anatomy
Authors: Helen Bynum
Journal: JAMA (2012)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
700. Formulation of Chemically Modified Short Interfering RNA (siRNA) for Local Delivery to the Respiratory Tract
Authors: Paul B. McCray, Brent A. Dickinson, Kim C. Hartsough, Anna M. Gomez, Sharon F. Jamison, Tim R. Shadel, Jennifer A. Lockridge, Pam A. Pavco, Wei-min Wang, Tongqian Chen, Chandra Vargeese, David K. Tung, Christine L. Wohlford-Lenane, Ivan M. Richards
Journal: Molecular Therapy (2006)

RNA interference using synthetic siRNAs to inhibit gene expression in a sequence-specific manner offers a novel opportunity for treating pulmonary diseases including asthma, airway inflammation, and respiratory viral infections. Developing siRNAs as therapeutics will however require chemical modifications to avoid degradation by nucleases, and delivery formulations to improve lung exposure. In the studies summarized here, we compared the time course of exposure in lung tissue of unmodified siRNA and greater than 95% stabilized siRNA. As part of a program to evaluate siRNAs that target inflammatory cytokines and their receptors, IL-4Ra siRNA, 20 μg total administered dose (1.0 mg/kg), was given intratracheally in anesthetized mice. siRNA in lung tissues was assessed at multiple time points after dosing using a hybridization assay. In addition, the time course of exposure in lung tissue of siRNA in proprietary lipid nanoparticle formulations were also evaluated. Chemical modification increased the half life of lung exposure of unstabilized siRNA from less than 2.0 min to 2.7h. Formulation of chemically modified siRNA increased the half life of lung exposure to 22h. Direct intratracheal adminstration of non- formulated Cy3-labeled siRNAs resulted in diffuse, dose-dependent uptake by respiratory epithelia and macrophages. Chemically modified, formulated siRNAs against specific targets will provide the tools for exploring the pathophysiology of lung diseases and have the potential to provide novel therapies. This work represents a collaboration between the University of Iowa and Sirna Therapeutics.
Periconceptional Use of Multivitamins and the Occurrence of Neural Tube Defects
Authors: Joseph Mulinare
Journal: JAMA (1988)

We studied the association between multivitamin use during the periconceptional period and the occurrence of neural tube defects using data from the Atlanta Birth Defects Case-Control Study. There were 347 babies with neural tube defects who were live born or stillborn to residents of metropolitan Atlanta from 1968 through 1980. The 2829 control-babies born without birth defects were randomly selected through birth certificates. Periconceptional multivitamin use was defined as reported use for each of the three months before conception through the first three months of pregnancy. Mothers who reported not using multivitamins any time during the six-month period were defined as nonusers. Fourteen percent of mothers reported periconceptional multivitamin use and 40% reported nonuse. Multivitamin users were different from nonusers in a number of demographic, health-related, and life-style characteristics. We found an overall apparent protective effect of periconceptional multivitamin use on the occurrence of neural tube defects, with a crude estimated relative risk of 0.40 (95% confidence interval, 0.25 to 0.63). At this time, it is not possible to determine whether this apparently lower risk is the direct result of multivitamin use or the result of other characteristics of women who use multivitamins.
Recent advances in minimal access surgery
Authors: A. Darzi, S. Mackay
Journal: BMJ (2002)

Minimally invasive surgery is the most important revolution in surgical technique since the early 1900s.Its development was facilitated by the introduction of miniaturised video cameras with good image reproduction.Laparoscopic cholecystectomy was the first procedure to be widely accepted, and several others are now well established.Other procedures are being validated, but further use of the technique may partly depend on the development of new enabling technologies.For example, a virtual reality laparoscopic simulator was recently used to assess the value of a three dimensional laparoscopic camera system. 1 We present an overview of advances in minimal access surgery, concentrating on procedures that have most recently become established in everyday surgical practice. MethodsWe selected the topics to be discussed after consultation with the other surgeons in our department.We then conducted a literature search (Medline
Exaggerated arthropod-bite lesions in patients with chronic lymphocytic leukemia: A clinical, histopathologic, and immunopathologic study of eight patients
Authors: Mark D.P. Davis, Charles Perniciaro, Patrick R. Dahl, Henry W. Randle, Marian T. McEvoy, Kristin M. Leiferman
Journal: Journal of the American Academy of Dermatology (1998)
Time to think Ebola: a message from NHS England to frontline clinical staff
Authors: R. P. Finn, C. Smith, S. Ghafur, A. Zarkali, K. Adlington, B. Winter, B. E. Keogh
Journal: BMJ (2014)

Professor Sir Bruce Keogh, the national medical director, and Dr Bob Winter, the national clinical director for emergency preparedness, have released an important announcement on Ebola to frontline staff.1 2 In line with the key messages from the guidance on Ebola from Public Health England and the guidelines from the College of Emergency Medicine,3 4 it highlights the utmost importance …
An ambulant porcine model of acid reflux used to evaluate endoscopic gastroplasty
Authors: S S Kadirkamanathan, E Yazaki, D F Evans, C C Hepworth, F Gong, C P Swain
Journal: Gut (1999)

There is a lack of suitable models for testing of therapeutic procedures for gastro-oesophageal reflux disease. Endoscopic sewing methods might allow the development of a new less invasive surgical approach to treatment of gastrointestinal disorders.To develop an animal model of gastro-oesophageal reflux for testing the efficacy of a new antireflux procedure, endoscopic gastroplasty, performed at flexible endoscopy without laparotomy or laparoscopy.At endoscopy a pH sensitive radiotelemetry capsule was sewn to the oesophageal wall, 5 cm above the lower oesophageal sphincter, in six large white pigs. Ambulant pH recordings (48-96 hours; total 447 hours) were obtained. The median distal oesophageal pH was 6.8 (range 6.4-7.3); pH was less than 4 for 9.3% of the time. After one week, endoscopic gastroplasty was performed by placing sutures below the gastro-oesophageal junction, forming a neo-oesophagus of 1-2 cm in length. Postoperative manometry and pH recordings (24-96 hours; total 344 hours) were carried out.Following gastroplasty, the median sphincter pressure increased significantly from 3 to 6 mm Hg and in length from 3 to 3.75 cm. The median time pH was less than 4 decreased significantly from 9.3% to 0.2%.These are the first long term measurements of oesophageal pH in ambulant pigs. The finding of spontaneous reflux suggested a model for studying treatments of reflux. Endoscopic gastroplasty increased sphincter pressure and length and decreased acid reflux.