tbuckley/cabot-search huggingface dataset:
Abstract 4210: An osteopontin promoter polymorphism is associated with aggressiveness in breast cancer.
Authors: Georg F. Weber, Divya Ramchandani
Journal: Cancer Research (2013)

Abstract Background: Like cancer initiation, cancer progression and metastasis are genetically programmed. However, unlike oncogenes metastasis genes are deregulated in cancer by aberrant expression or splicing. We have previously described splicing of the metastasis gene osteopontin in cancers. Here we have analyzed polymorphic sites in the osteopontin promoter as potential contributors to aberrant expression in breast cancers. Methods: This study comprised 241 breast cancer specimens, for 111 of which DNA from normal surrounding tissue was available, and 65 healthy breast samples. One probe set tested the polymorphic promoter sites -66, -443, -1748 and -1776. For comparison, we also investigated non-synonymous DNA sequence variations in the coding region positions 305, 367, 794 and 1025. Results: The polymorphic sites in positions -66 and 305 were not in Hardy-Weinberg equilibrium and hence were not included in further analyses. The polymorphic site in position -443 of the promoter was associated with tumor grade. Expectedly, there was no association of the promoter SNPs with tumor stage or with in situ carcinoma versus cancer, as stage and early transformation are determined by the sampling time more than by tumor genetics. In a subset of samples, osteopontin expression levels had previously been obtained. The allelic distribution in positions -443 and -1748 was distinct between high and low expressors, confirming the importance of promoter SNPs. These two sites also form a haplotype. Osteopontin expression has been associated with breast cancer progression, regardless of the histologic subtype of the cancer. Remarkably, the polymorphic site at -443, but not -1748 or -1776, showed differences between ER positive and ER negative breast cancers and between PR positive and PR negative breast cancers, but there was no association with HER2 status. In five cases, the genotype of the tumor was different from the genotype of the host, implying the possibility of somatic mutations in the osteopontin promoter that may affect expression. The polymorphic sites in positions 367, 794, and 1025 showed one homozygous genotype for all specimens in this study. Conclusion: Our results show that the osteopontin promoter SNPs -443 (rs11730582) and -1748 (rs2728127) are important for gene expression and tumor aggressiveness. Citation Format: Georg F. Weber, Divya Ramchandani. An osteopontin promoter polymorphism is associated with aggressiveness in breast cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 4210. doi:10.1158/1538-7445.AM2013-4210
An Anatomy of Addiction: Sigmund Freud, William Halsted, and the Miracle Drug Cocaine
Authors: A. Mark Clarfield
Journal: JAMA (2011)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Genotype dependence of hepatitis C virus antibodies detectable by the first-generation enzyme-linked immunosorbent assay with C100-3 protein.
Authors: R Nagayama, F Tsuda, H Okamoto, Y Wang, T Mitsui, T Tanaka, Y Miyakawa, M Mayumi
Journal: Journal of Clinical Investigation (1993)

Hepatitis C virus (HCV) samples in 155 sera, from patients with chronic non-A, non-B liver disease and blood donors, were grouped into four genotypes (I, II, III, and IV) by amplification of core-gene sequences by polymerase chain reaction with type-specific primers. HCV genotypes were compared with various HCV-associated antibodies detectable by the first-generation ELISA (ELISA-1) with C100-3 protein and a second-generation immunoblot assay with four recombinant HCV proteins. Antibodies to C100-3 protein and those to its subsequence (5-1-1) were detected in 13 (93%) and 12 (86%), respectively, of 14 sera with genotype I HCV; 56 (79%) and 58 (82%) of 71 sera with genotype II; 13 (34%) and 6 (16%) of 38 sera with genotype III; and 11 (34%) and 4 (13%) of 32 sera with genotype IV. Amino acid sequences of C100-3 of genotype I HCV are conserved by approximately 90% in genotype II, but only by approximately 75% in genotypes III and IV. The sensitivity of ELISA-1, therefore, would be influenced by heterogeneity in C100-3 sequences of different genotypes.
Woodchuck hepatitis virus-induced carcinoma as a relevant natural model for therapy of human hepatoma
Authors: C. Gouillat, D. Manganas, F. Zoulim, D. Vitrey, G. Saguier, M. Guillaud, J.F. Ain, R. Duque-Campos, C. Jamard, M. Praves, C. Trepo
Journal: Journal of Hepatology (1997)
Clinical and histological features retain their prognostic impact under interferon therapy of CML: A pilot study
Authors: Juergen Thiele, Hans Michael Kvasnicka, Norbert Niederle, Otto Kloke, Marcus Schmidt, Heiko Lienhard, Thomas Zirbes, Raoul Boris Meuter, Lutz Dietrich Leder, Robert Fischer
Journal: American Journal of Hematology (1995)

Abstract In 55 patients with Ph 1+ CML under interferon (IFN) monotherapy, an immunohistochemical and morphometric study on pretreatment bone marrow biopsies was performed to evaluate the prognostic impact of clinical as well as histological disease features. For identification of megakaryocytes we used the PAS stain and CD61 to calculate the subtraction of precursors (pro‐ and megakaryoblasts). Demonstration of macrophages and their different subsets was carried out by PG‐M1 (CD68) and the GSA‐I lectin. The erythroid precursors were stained by Ret40f (anti‐glycophorin C). Density of argyrophilic (reticulin plus collagen) fibers was determined by applying Gomori's silver impregnation method. Clinical variables like state of hematological response to IFN administration, age, spleen and liver size, myeloblasts plus promyelocytes, basophils as well as basophils and eosinophils exerted a predictive capacity by univariate statistical analysis. However, when entering these factors into previously published risk models, i.e., the so‐called Sokal score and its modifications, to assess subgroups with different survival patterns or relative risk groups, a clear‐cut discrimination was not feasible. Bone marrow features of prognostic value consisted of megakaryocytes and their precursors, fibers, and pro‐ and erythroblasts. Only when including histological variables into a formerly reported Cox model, could a significant separation of patients into the different categories or relative risk groups be computated. In conclusion, the present data emphasize the prognostic impact of histological parameters to be considered in all clinical trials on CML.
451 EVALUATION OF PHARMACOKINETIC DRUG–DRUG INTERACTION (DDI) BETWEEN BMS-791325, AN NS5B NON-NUCLEOTIDE POLYMERASE INHIBITOR, DACLATASVIR AND ASUNAPREVIR IN TRIPLE COMBINATION IN HCV GENOTYPE 1-INFECTED PATIENTS
Authors: X. Wang, W. Li, S.-P. Huang, B. He, E. Chung, A. Griffies, E. Cooney, E. Hughes, H. Kandoussi, K. Sims, D. Gardiner, R. Bertz, T. Eley
Journal: Journal of Hepatology (2013)
Effect of inhaled piriprost (U-60, 257) a novel leukotriene inhibitor, on allergen and exercise induced bronchoconstriction in asthma.
Authors: J S Mann, C Robinson, A Q Sheridan, P Clement, M K Bach, S T Holgate
Journal: Thorax (1986)

The leukotrienes, a group of oxidative metabolites of arachidonic acid, have potent pharmacological actions on human airways. We have investigated the effects of a leukotriene synthesis inhibitor, piriprost (U-60, 257) administered by inhalation on allergen and exercise induced bronchoconstriction in 12 subjects with allergic asthma. Subjects underwent diagnostic challenges with allergen and treadmill exercise to define the strengths of the stimuli required to reduce the FEV1 to about 25% of baseline (PS25). On separate study days subjects inhaled either piriprost 1 mg or vehicle placebo, followed 15 minutes later by the PS25 allergen or exercise. The FEV1 was measured at regular intervals before and after challenge up to 60 minutes. After allergen challenge in six subjects peak expiratory flow (PEF) was measured for the following 20 hours. When compared with placebo, inhalation of piriprost had no significant protective effect on the fall in FEV1 at any time point within 60 minutes of allergen or exercise challenge. In the four subjects with a documented late asthmatic reaction 2-12 hours after allergen challenge piriprost had no protective effect when compared with placebo. In the subjects who recorded PEF over 20 hours after allergen challenge there was no significant difference between piriprost and placebo. Piriprost was appreciably more irritant to the respiratory tract than was placebo. On the assumption that inhaled piriprost was bioavailable in the airways, this study casts doubt on any theory of a pivotal role for leukotrienes in the pathogenesis of acute exercise and allergen induced airway bronchoconstriction in asthma.
Properties of the hyperpolarization-activated current (If) in isolated mouse sino-atrial cells
Authors: Matteo E. Mangoni, Joël Nargeot
Journal: Cardiovascular Research (2001)

Objective: We have investigated the properties of the hyperpolarization-activated (If) current in pacemaker cells from the mouse sino-atrial node (SAN). Methods: The If current was studied in cells isolated enzymatically from the SAN region of adult C57BL6/J mice. The whole-cell variation of the patch-clamp technique was employed to investigate the basic properties of If. Results: In mouse SAN cells, the If current density at −120 mV was 18±2 pA/pF (n=23). If was not detected in cells showing atrial-like morphology that were also found in SAN preparations (n=7). If was blocked by 5 mM Cs+, was inhibited by application of 5 μM acetylcholine, and was increased by 10 μM noradrenaline. The If current reversal potential was −31±2 mV under physiological concentration of Na+ and K+ ions. Lowering the extracellular Na+ concentration reduced If amplitude, while increased when the extracellular K+ concentration was augmented. If voltage for half activation was −87±1 mV (n=6). Conclusions: We conclude that the native If current in mouse SAN cells shows functional properties that are similar to If described in rabbit SAN tissue. This study opens the possibility of investigating the involvement of If in the regulation of heart rate in genetically modified mice.
Infusion of ex vivo expanded T regulatory cells in adults transplanted with umbilical cord blood: safety profile and detection kinetics
Authors: Claudio G. Brunstein, Jeffrey S. Miller, Qing Cao, David H. McKenna, Keli L. Hippen, Julie Curtsinger, Todd DeFor, Bruce L. Levine, Carl H. June, Pablo Rubinstein, Philip B. McGlave, Bruce R. Blazar, John E. Wagner
Journal: Blood (2010)
T-Cell/Histiocyte-Rich Large B-Cell Lymphoma: A Distinct Clinicopathologic Entity
Authors: R. Achten, G. Verhoef, L. Vanuytsel, C. De Wolf-Peeters
Journal: Journal of Clinical Oncology (2002)

PURPOSE: Although it has proven difficult to delineate diagnostically reproducible and clinically relevant subgroups, the heterogeneity of diffuse large B-cell lymphomas (DLBCL) is widely acknowledged. In 1992, we reported on six cases that suggested that large B-cell lymphoma rich in stromal histiocytes and T cells may be identified as a distinct clinicopathologic entity within DLBCL. PATIENTS AND METHODS: An integrated clinicopathologic study of 40 cases of this DLBCL subtype is presented. RESULTS: Distinguishing a DLBCL rich in histiocytes and reactive T cells, designated T-cell/histiocyte–rich large B-cell lymphoma (THR-BCL), may be justified from a clinical point of view. The disease typically affects middle-aged male patients who usually present with advanced-stage disease that is not adequately managed with current therapeutic strategies. Whereas proliferation fraction and p53 overexpression, in addition to the clinical variables incorporated in the International Prognostic Index (IPI), significant...
THE CLEAN INUNCTION TREATMENT OF SYPHILIS WITH MERCURY
Authors: H. N. COLE
Journal: JAMA (1924)

In a preliminary paper, we 1 pointed out that the mercury in the practice of mercurial inunction is probably absorbed only from that part of the ointment which has been rubbed into the hair follicles and into the entrance of the sebaceous and sweat glands; and that therefore any mercury remaining on the surface of the skin, after the rubbing is stopped, would be only an objectionable nuisance, without any therapeutic effect.  In order to test this assumption, a series of forty-four patients was treated with mercury rubs in the usual manner, except that the skin was carefully cleansed with benzin at the end of the half-hour rubs, so as to remove all mercury that had remained on the surface.  The results bore out our expectations: Mercury absorption, as judged by gingivitis, etc., occurred in the same proportion of cases, and after the same average number of rubs, as one
Nanofluidic Digital PCR for Improved Selection of Metastatic Colorectal Cancer Patients to Anti-EGFR Therapies
Authors: Daniel Azuara, Cristina Santos, Adriana Lopez-Doriga, Robert Montal, Marga Nadal, Xavier Sanjuan, Victor Moreno, Josep Tabernero, Clara Montagut, Ramon Salazar, Gabriel Capellá
Journal: Annals of Oncology (2013)
Cardiopulmonary Resuscitation— Potential Danger of Cross-Infection
Authors: Abdul H. Khan
Journal: JAMA (1979)

<h3>To the Editor.—</h3> Cardiopulmonary resuscitation (CPR) training is conducted with the aid of a manikin using the guidelines of the American Heart Association.<sup>1</sup>The procedure entails mouth-to-mouth breathing and cardiac compression, performed either as a single-person or as a twoperson effort. The latter requires frequent switching of roles by the two "rescuers" from breathing to cardiac compression without allowing time to clean the manikin's oral surface. Thus, the contamination of manikins by saliva poses a potential threat of infection. In a previous report, the Center for Disease Control<sup>2</sup>indicated that the chances of transmitting hepatitis via manikins contaminated with hepatitis B surface antigenpositive saliva were remote. We address ourselves to the potential danger of bacterial infection during a CPR training session. The initial study consisted of 30 physicians and six instructors (six persons to a manikin). Cultures taken from the manikin lips at the conclusion of the CPR
Comments on "exposing ourselves to art".
Authors: A L Weiss, R J Jaeger
Journal: Environmental Health Perspectives (1997)

We believe that it is important for creative individuals to be encouraged in their expression.The driving force behind an artists' quest for new and innovative means to express his or her creativity should not be unnecessarily impeded.On the other hand, a proper understanding of the materials and their hazards when used in the creation of art is necessary.Since the proper expression of likely risks is vitally important, we are concerned that individual examples may have been presented in a manner that makes them appear sensational or of concern beyond the risks expected.In particular, we believe that formula review and supervision by a toxicologist is a sound means to pre- vent harm to the public.Such supervision and review should be more widely practiced for a greater variety of materials.The toxic effects exhibited by improper or excessive exposure to art materials are as varied as the materials themselves.As toxi- cologists responsible for art materials label- ing under the Labeling of Hazardous Art Materials Act (LHAMA), we know that cer- tain toxic chemicals cause acute effects (immediate reaction after one exposure).These are easy to spot, and warnings about acute hazards are defined by the Federal Hazardous Substances Act (FHSA), among others.Other effects, more subtle or less timely, can be delayed, and these chronic reactions that occur after multiple periods of use constitute the area of chronic health concern mandated by LHAMA.Perhaps justifiably, the author cites Monona Rossol and Michael McCann in their expressions of concern for the chronic hazards in the artistic community.Both are known in the art hazard field and have been vocal advocates of art safety for many years.As practicing toxicologists, we under- stand that some effects are reversible, while others may not be unless caught in time.Some materials provide intrinsic warnings,
Effects of N-methyl hexanoylhydroxamic acid (NMHH) and myoglobin on endothelial damage by hydrogen peroxide
Authors: D P D. Bono, W D Yang, M J Davies, C S Collis, C A R. Evans
Journal: Cardiovascular Research (1994)

Objective: The aim was to investigate the interaction of the novel antioxidant N-methyl hexanoylhydroxamic acid (NMHH) with myoglobin in protecting endothelial cells against H2O2 mediated damage. Methods: Cultured bovine aortic endothelial cells were exposed to 50–100 μM H2O2 for 10–60 min with and without NMHH and/or myoglobin, and immediate or delayed damage was assessed by lactate dehydrogenase release, 3H adenine uptake, a tetrazolium reduction assay, and microscopy. Results: Brief exposure to low concentrations of H2O2 caused cell damage, for which the tetrazolium reduction assay was the most sensitive assay, and inhibited subsequent cell division. NMHH in concentrations from 50 to 200 μM protected against damage provided it was present at the time of adding H2O2, and the effect was markedly potentiated by 10 μM oxymyoglobin, which had little protective effect alone. Conclusions: NMHH is an effective antioxidant which is markedly potentiated by low concentrations of oxymyoglobin. Oxymyoglobin may potentiate NMHH by scavenging H2O2 through the rapid formation of ferrylmyoglobin, which is then reduced by NMHH. This synergism may be particularly relevant to the protection of myoglobin-rich cells such as myocytes. Cardiovascular Research 1994;28:1641-1646
Promoting partnerships: challenges for the internet age
Authors: A. R Jadad
Journal: BMJ (1999)

The internet is transforming health care. It is creating a new conduit not only for communication but also in the access, sharing, and exchange of information among people and machines. Although it is impossible to predict its evolution, recent developments indicate that the internet will have a profound effect on the way that patients and clinicians interact. It will also foster a new level of knowledge among patients, enable them to have input into making decisions about their health care, and allow them to participate in active partnerships with many groups of decision makers such as clinicians, policymakers, and researchers.

This article describes the 10 key challenges that must be met to allow optimal partnerships to develop between patients and clinicians. This list of challenges, which is by no means comprehensive, is intended to stimulate discussion of the issues that require immediate attention to maximise the potential benefits of the internet for health care. Access to technology and information must be equitably distributed.

#### Summary points

The internet provides opportunities to build strong partnerships between patients and clinicians

There should be more collaboration between consumer groups and professional organisations

To be effective, information systems must be easy to access and use and must provide rapid access to appropriate information

There needs to be better integration of information and the values of the people making decisions about their healthcare needs

In the internet era it is important for patients and clinicians to work together as partners. However, there is little evidence in the peer reviewed literature that we are moving beyond the political correctness of stating these sentiments. A search of Medline, CINAHL, and Healthstar from the year of the first issue to May 1999 and of the Cochrane Library's first issue for 1999 using the terms “consumer or public or patient” and “Internet …
A neglected but prevalent tragedy
Authors: F. Diadhiou, N. Goyaux, O. Faye, P. Thonneau
Journal: BMJ (1999)

Aminata, aged 24, was admitted as an emergency suffering from fever and severe pelvic pain in February 1998. Medical examination showed signs of peritonitis and foetid leucorrhoea. She told the doctor that she had had an illegal abortion after 12 weeks of amenorrhoea. An emergency laparotomy detected an abdominal cavity filled with pus (>300 ml), many adhesions, and a 3 cm perforation of the uterus around the isthmus.

She had a hysterectomy, a left annexectomy, and a right salpingectomy. Careful peritoneal cleaning, prolonged drainage, and a long course of strong antibiotics simplified the postoperative procedure. She returned home two weeks later.

Aminata was born in Dakar. She received several years of primary education, married at the age of 17, and had …
Penicillinase-producing Neisseria gonorrhoeae in Great Britain, 1977-81: alarming increase in incidence and recent development of endemic transmission.
Authors: J A McCutchan, M W Adler, J R Berrie
Journal: BMJ (1982)

Since penicillinase-producing Neisseria gonorrhoeae appeared five years ago in West Africa and South-east Asia reported cases have doubled annually in Great Britain, primarily as a result of increasing importation. Importation of penicillinase-producing Neisseria gonorrhoeae has increased exponentially because dramatic expansion of these strains in their regions of origin has led to increasing infection of male air travellers. From 1977 to 1980 infections acquired in Great Britain played only a minor part in the exponential increase. During 1981 the number of indigenous cases increased much more rapidly than imported cases, indicating that these strains have become truly endemic in Great Britain. Currently, identification of patients at high risk and initial treatment with penicillinase-resistant antibiotics offers the best hope of containing the strains. The emergence and rapid spread of penicillinase-producing Neisseria gonorrhoeae shows the international consequences of the abuse of antibiotics.
A Critical Role for Notch Signaling in the Formation of Cholangiocellular Carcinomas
Authors: Steffen Zender, Irina Nickeleit, Torsten Wuestefeld, Inga Sörensen, Daniel Dauch, Przemyslaw Bozko, Mona El-Khatib, Robert Geffers, Hueseyin Bektas, Michael P. Manns, Achim Gossler, Ludwig Wilkens, Ruben Plentz, Lars Zender, Nisar P. Malek
Journal: Cancer Cell (2013)