tbuckley/cabot-search huggingface dataset:
Analysis and Correction of Pressure Wave Distortion in Fluid-Filled Catheter Systems
Authors: HERMAN L. FALSETTI, ROBERT E. MATES, ROBYN J. CARROLL, RAMJI L. GUPTA, ADAM C. BELL
Journal: Circulation (1974)

Dynamic characteristics of a variety of catheters and their dependence on various operating parameters are presented. Careful flushing of the catheter with degassed water or saline considerably improves the performance of these systems. The operating temperature, number of catheter uses, and average transmural pressure difference do not have a significant effect. The error due to catheter distortion in commonly used left ventricular performance indices (end diastolic pressure, peak systolic pressure, maximum dp/dt and V max ) was assessed. On the basis of these results, it is shown that the natural frequency of the catheter system must be greater than 40 Hz to produce results accurate to 10%. The experimental data were used to develop a semi-empirical model for the catheter transducer system. This model was used in the design of an analog compensator to further improve the dynamic characteristics of the catheter transducer system. The compensator requires only two parameter settings, catheter natural frequency and damping ratio.
Myofibrillar calcium sensitivity of isometric tension is increased in human dilated cardiomyopathies: role of altered beta-adrenergically mediated protein phosphorylation.
Authors: M R Wolff, S H Buck, S W Stoker, M L Greaser, R M Mentzer
Journal: Journal of Clinical Investigation (1996)

To examine the role of alterations in myofibrillar function in human dilated cardiomyopathies, we determined isometric tension-calcium relations in permeabilized myocytesized myofibrillar preparations (n = 16) obtained from left ventricular biopsies from nine patients with dilated cardiomyopathy (DCM) during cardiac transplantation or left ventricular assist device implantation. Similar preparations (n = 10) were obtained from six normal hearts used for cardiac transplantation. Passive and maximal Ca2+-activated tensions were similar for the two groups. However, the calcium sensitivity of isometric tension was increased in DCM compared to nonfailing preparations ([Ca2+]50=2.46+/-0.49 microM vs 3.24+/-0.51 microM, P < 0.001). In vitro treatment with the catalytic subunit of protein kinase A (PKA) decreased calcium sensitivity of tension to a greater degree in failing than in normal preparations. Further, isometric tension-calcium relations in failing and normal myofibrillar preparations were similar after PKA treatment. These findings suggest that the increased calcium sensitivity of isometric tension in DCM may be due at least in part to a reduction of the beta-adrenergically mediated (PKA-dependent) phosphorylation of myofibrillar regulatory proteins such as troponin I and/or C-protein.
Pathologic Fractures in a Patient with Renal Osteodystrophy
Authors: RICHARD J. CAMPEAU, RICHARD D. BELLAH, DATLA G.K VARMA
Journal: Clinical Nuclear Medicine (1987)

A case of false–negative Tc–99m MDP bone scintigrams, taken at one and two weeks for pathologic fractures in a patient with metabolic bone disease and a super–scan appearance, is described. The patient had renal osteodystrophy, and postparathyroidectomy hypocalcemia. Postoperative seizures caused multiple pathologic fractures. Initial scans were negative for focal tracer localization in the presence of a continued super-scan appearance. After months of calcium and vitamin D replacement therapy, fracture sites became positive on Tc–99m MDP imaging. The observations in this case lend credence to the hypothesis of Tc–99m MDP binding by immature collagen in the production of a super scan in metabolic bone disease, as well as that of Tc-99m MDP chemisorption to calcium hydroxyapatite crystal in fracture healing. In addition, aluminum toxicity, common in chronic renal osteodystrophy, may have played a role in the delayed fracture healing.
The heat shock cognate protein 70 is associated with hepatitis C virus particles and modulates virus infectivity
Authors: Romain Parent, Xiaoyu Qu, Marie‐Anne Petit, Laura Beretta
Journal: Hepatology (2009)

There is growing evidence that virus particles contain host cell proteins. These proteins may provide viruses with means to evade the immune system or with mechanisms for cell entry and release. A proteomic analysis performed on highly purified hepatitis C virus (HCV) J6/JFH virions identified the heat shock cognate protein 70 (HSC70) as part of the viral particles. These results were further validated via immunogold electron microscopy. The HSC70 interaction HPD motif was found present on the E2 envelope of the J6/JFH strain, as well as in over 50% of genotype 2 clinical HCV isolates. In addition, HSC70 was found associated with viral particles from an HCV genotype 2a-infected patient. Preincubation of HCV particles with anti-HSC70 antibodies decreased viral infectivity. Within infected cells, colocalization of HSC70 with the HCV core and E2 proteins was observed around lipid droplets. Reduction of HSC70 expression using an RNA interference approach decreased the volume of lipid droplets as well as viral release without affecting HCV replication levels.These results suggest that HSC70 modulates HCV infectivity and lipid droplet-dependent virus release.
Detection and identification of tumor-associated protein variants in human hepatocellular carcinomas
Authors: Evelyn Zeindl-Eberhart, Sibylle Haraida, Sibylle Liebmann, Peter Roman Jungblut, Stephanie Lamer, Doris Mayer, Gundula Jäger, Stephen Chung, Hartmut Manfred Rabes
Journal: Hepatology (2004)

The proteomic approach is a valuable tool to detect and identify proteins that are associated with cancer. In previous investigations on experimentally induced rat hepatomas, we detected aldose reductase-like protein (ARLP) as a highly significant marker protein. Our present study was intended to look for the presence of similar tumor-associated marker proteins on human hepatocellular carcinomas (HCC). We found several novel tumor-associated protein variants that represent members of the aldo-keto reductase (AKR) superfamily. Human aldose reductase-like protein-1 (hARLP-1) was the most prominent tumor-associated AKR member detected in HCC by 2-dimensional electrophoresis (2-DE) and identified by mass spectrometric fingerprinting. The enzyme was found in 4 distinct forms (hARLP-1, 36/7.4 (kd/pI); hARLP-2, 36/7.2; hARLP-3, 36/6.4; and hARLP-4, 33/7.35). In addition, a human aldose reductase-like protein (hARLP-5, 36/7.6) was identified that differed from hARLP-1 by 1 amino acid (D313N), indicating 2 allelic forms of the human aldose reductase-like gene. A novel antibody directed against common parts of the hARLPs revealed hARLP reactivity in human HCC by immunohistochemistry. Furthermore, aldose reductase (AR) was identified and characterized as a tumor-associated variant. In conclusion, in all investigated human HCCs at least one of the various types of the described tumor-associated proteins of the AKR superfamily was clearly present. Of these HCC samples, 95% were positive for hARLPs as proven by 2-DE analysis and/or by use of the antibody directed against hARLP. Thus, hARLP is a strong candidate for use as an immunohistochemical diagnostic marker of human HCC.
Melanoma Antigen-11 Inhibits the Hypoxia-Inducible Factor Prolyl Hydroxylase 2 and Activates Hypoxic Response
Authors: Olga Aprelikova, Silvia Pandolfi, Sean Tackett, Mark Ferreira, Konstantin Salnikow, Yvona Ward, John I. Risinger, J. Carl Barrett, John Niederhuber
Journal: Cancer Research (2009)

Abstract Activation of hypoxia-inducible factors (HIF), responsible for tumor angiogenesis and glycolytic switch, is regulated by reduced oxygen availability. Normally, HIF-α proteins are maintained at low levels, controlled by site-specific hydroxylation carried out by HIF prolyl hydroxylases (PHD) and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitin ligase. Using a yeast two-hybrid screen, we identified an interaction between melanoma antigen-11 (MAGE-11) cancer-testis antigen and the major HIF-α hydroxylating enzyme PHD2. The interaction was confirmed by a pull-down assay, coimmunoprecipitation, and colocalization in both normoxic and hypoxic conditions. Furthermore, MAGE-9, the closest homologue of MAGE-11, was also found to interact with PHD2. MAGE-11 inhibited PHD activity without affecting protein levels. This inhibition was accompanied by stabilization of ectopic or endogenous HIF-1α protein. Knockdown of MAGE-11 by small interfering RNA results in decreased hypoxic induction of HIF-1α and its target genes. Inhibition of PHD by MAGE-11, and following activation of HIFs, is a novel tumor-associated HIF regulatory mechanism. This finding provides new insights into the significance of MAGE expression in tumors and may provide valuable tools for therapeutic intervention because of the restricted expression of the MAGE gene family in cancers, but not in normal tissues. [Cancer Res 2009;69(2):616–24]
Increased Risk for Spondylitis Stigmata in Apparently Healthy HL-AW27 Men
Authors: LEWIS M. COHEN
Journal: Annals of Internal Medicine (1976)

Evidence for ankylosing spondylitis was sought by clinical, radiologic, and ophthalmologic examination in HL-A W27-positive men, aged 18 or older, selected from a tissue-donor population. Back pain of 3 months' duration or longer (P < 0.05), back stiffness, restricted lumbar flexion and chest expansion, sacroiliac erosions (P < 0.05) and sclerosis, and ophthalmologic sequels of anterior uveitis were found more often in the 24 men of the W27 group than in a control group of 31 men lacking this antigen. Based upon accepted criteria, 3 W27 persons had definite spondylitis and an additional 3 W27 persons and one control subject had findings strongly suggestive of spondylitis (P < 0.05). This striking frequency, if extrapolated to the general population, would place approximately 1 of 4 W27-positive men at risk for this disease.
The Serum Leucine Aminopeptidase Test
Authors: J. Harkness, B. W. Roper, J. A. Durant, H. Miller
Journal: BMJ (1960)

CARCINOMABRrnsH 1787 in the uterine preparation is indeed present in the serum of 95% of patients with cancer, as Makari claims. SummaryA preliminary attempt to use the Schultz-Dale technique in the diagnosis of cancer has been successful in 23 cases of carcinoma, and unsuccessful in 26.The claims of Makari and of Burrows that the test has an accuracy of over 95% have not been substan- tiated.On the other hand, the results can be taken to confirm the immunological basis of the method.We are grateful to the consulting physicians and surgeons of the Royal Adelaide Hospital and of the Queen Elizabeth Hospital, Adelaide, who suggested that we should test serum from their cases; to Professor R. F.
Influence of Apoptosis and Cell Cycle Regulator Proteins on Chemotherapy Response and Survival in Stage IIIA/IIIB NSCLC Patients
Authors: José L. Morero, Claudia Poleri, Claudio Martín, Maximiliano Van Kooten, Reynaldo Chacón, Moisés Rosenberg
Journal: Journal of Thoracic Oncology (2007)
Cognitive Measures of Vietnam-Era Prisoners of War
Authors: D. Williams
Journal: JAMA (2002)

Abstract : Experience as a prisoner of war (POW) could lead to cognitive impairment because of the injuries, including head trauma, and other stressors endured preceding and during capture, and during incarceration. Some research has reported decreased cognitive performance in repatriated POWs (RPOWs), while other research has found no cognitive impairment. The objective of this research is to assess the relative cognitive status of U.S. Navy Vietnam-era RPOWs using extant data from the Naval Operational Medicine Institute. Performance on 3 cognitive batteries was compared between RPOWs and a control group. These batteries were the Haistead-Reitan Neuropsychological Battery (HRNB), the Wechsler Adult Intelligence Scale (WMS), and the CogScreen-Aeromedical Edition (AE). The HRNB and WMS were each administered 3 times across 20 years, and the CogScreen-AE was administered once. The few significant differences between the cognitive performance of RPOWs and their matched controls did not indicate evidence of performance decrement for RPOWs on any cognitive measure. Results suggest that this group of RPOWs did not experience cognitive impairment as a result of their experience as POWs.
Experience with diazepam in tetanus.
Authors: D. Femi-Pearse
Journal: BMJ (1966)

Group A Group B Mild to Moderate P.U.O.; sudden death more than Overt kwashiorkor and multipie 1 week after tetanus symptoms abscesses . .I controlled .. . 1 Staphylococcal septicaemia, severe Bronchopneumonia 2 bronchopneumonia, Hb 30", and dehydration on admission.. Purulent laryngo-tracheobronchitis on admission; died within 36 hours I Died suddenly at home on day of discharge from hospital 1 Severe Bronchopneumonia and cardiac failure... 1Comment on Action of Diazepam Diazepam appeared to be of most value in cases of tetanus in which tonic spasm of muscles was marked but convulsive spasms were infrequent.It was effective in relieving trismus and opisthotonos in the majority of cases, at the dose of 4.4 mg./kg./24hours.The oral rout of administration was used almost exclusively in this trial and appeared to be perfectly satisfactory.Our limited experience of the intramuscular route of administration does not suggest that this has any special advantage over oral administration.The intravenous route of administration was tried on a few occasions and did not appear to produce a dramatic onset of action of the drug.No toxic effects attributable to diazepam were observed in either neonates or older children.The lower temperatures revealed among neonates who received diazepam, however, suggest that it tends to induce hypothermia.Mortality was not adversely affected by this action of the drug. Summary and ConclusionsResults of therapeutic trial of diazepam in tetanus are reported.The trial included 149 patients-104 neonates and 45 older children.Patients were randomly allocated into two treatment groups, one of which received diazepam in addition to the standard treatment used in both groups.In neonatal tetanus the mortality rate was unaffected by the use of diazepam, but the drug was of value in relieving tonic muscle spasm, in particular trismus.A significant number of patients who received diazepam had minimum temperatures of 95°F.(350 C.) and lower, but did not appear to be adversely affected by this.In older children with tetanus the overall mortality rate was lower among those who received diazepam than among those who did not, but the difference is not statistically significant.As in neonates, the drug was of value in relieving tonic spasm but did not seem to have any direct effect on convulsive spasms.Findings in this trial indicate that diazepam is a useful drug in the management of tetanus, principally because of its ability to reduce tonic muscle spasm.Its use appears to be free of toxic effects or unpleasant side-actions.In older children it may have favourably influenced mortality from the disease.
Cancer of the Prostate
Authors: Peter Goodwin
Journal: JAMA (1992)

To the Editor.  —We were confused by the recommendations contained in the Editorial by Oesterling1concerning the evaluation of older males for prostatic carcinoma.  The article by Johansson et al2demonstrated a 10-year disease-specific survival rate of 86.8%, with 19 of 223 patients dying of their prostatic cancer, while 105 died from other causes. The authors mentioned ongoing trials in Scandinavia comparing radiation with no initial treatment, and radical prostatectomy with deferred treatment. They contend that without evidence of benefit, the radical treatment of early stage prostatic cancer remains highly controversial.  In a Preliminary Communication, Carter et al3provide evidence that the annual rate of change in PSA levels was significantly greater in subjects with prostatic cancer compared with normal subjects and those with benign prostatic hypertrophy. They emphasize the lack of specificity (60%) and the relative insensitivity (78%) of PSA testing on a onetime basis, when
Lymphogranuloma Venereum Outbreak in a University Community
Authors: Bruce A. McLelland
Journal: JAMA (1976)

One aspect of the current epidemic of venereal diseases is the spread of lymphogranuloma venereum (LGV). Formerly confined to the lower echelons of society, it now affects more affluent classes as well. A cluster of four cases was seen in a small college town. Serial LGV complement fixation tests, the most valuable diagnostic tool, may reveal many more cases than reported previously. Early treatment is important. We recommend therapy for all persons sexually exposed to LGV who have any LGV complement fixation test anomaly, whether or not clinical signs develop.
Common Genetic Variation in <i>GLP1R</i> and Insulin Secretion in Response to Exogenous GLP-1 in Nondiabetic Subjects
Authors: Airani Sathananthan, Chiara Dalla Man, Francesco Micheletto, Alan R. Zinsmeister, Michael Camilleri, Paula D. Giesler, Jeanette M. Laugen, Gianna Toffolo, Robert A. Rizza, Claudio Cobelli, Adrian Vella
Journal: Diabetes Care (2010)

OBJECTIVE Glucagon-like peptide (GLP)-1 receptor is encoded by GLP1R. The effect of genetic variation at this locus on the response to GLP-1 is unknown. This study assessed the effect of GLP1R polymorphisms on insulin secretion in response to hyperglycemia and to infused GLP-1 in nondiabetic subjects. RESEARCH DESIGN AND METHODS Eighty-eight healthy individuals (aged 26.3 ± 0.6 years, fasting glucose 4.83 ± 0.04 mmol/l) were studied using a hyperglycemic clamp. GLP-1 was infused for the last 2 h of the study (0.75 pmol/kg/min over 121–180 min, 1.5 pmol/kg/min over 181–240 min). β-Cell responsivity (ΦTotal) was measured using a C-peptide minimal model. The effect of 21 tag single nucleotide polymorphisms (SNPs) in GLP1R on ΦTotal was examined. RESULTS Two SNPs (rs6923761 and rs3765467) were nominally associated with altered β-cell responsivity in response to GLP-1 infusion. CONCLUSIONS Variation in GLP1R may alter insulin secretion in response to exogenous GLP-1. Future studies will determine whether such variation accounts for interindividual differences in response to GLP-1–based therapy.
Murine hepatocyte cell lines promote expansion and differentiation of NK cells from stem cell precursors
Authors: Veronica Bordoni, Tonino Alonzi, Chiara Agrati, Fabrizio Poccia, Giovanna Borsellino, Giorgio Mancino, Gian Maria Fimia, Mauro Piacentini, Antonio Fantoni, Marco Tripodi
Journal: Hepatology (2004)

While fetal liver is a major hematopoietic organ, normal adult liver provides a suitable microenvironment for a variety of immune cells and, in several pathological conditions, may become a site of extramedullary hematopoiesis. The direct influence of hepatocytes on hematopoietic cell differentiation is poorly understood. We have previously reported that the Met murine hepatocyte (MMH) untransformed hepatocytic lines retain several morphological and functional features of hepatocytes in vivo and are able to support the survival, self-renewal, and differentiation of hematopoietic precursors in a cell-cell contact system. Here we report the effects of soluble factors released by MMH lines on bone marrow-derived cells. Lymphohematopoietic cells were cultured in two different cell contact-free systems: transwell inserts on MMH feeder layers, and MMH conditioned medium (MMH-CM). Both culture systems were able to promote a substantial expansion of bone marrow-derived cells and their differentiation to natural killer (NK) cells that express the NK1.1 and U5A2-13 markers. Purified hematopoietic stem cells (Sca-1+Lin-), either plated as a bulk population or as single cells, were also able to differentiate into NK cells, when cultured in MMH-CM; thus, soluble factors secreted by MMH lines promote the expansion and differentiation of NK precursor cells. MMH-CM-derived NK cells are functionally active; stimulation by interleukin (IL)-12 together with IL-18 was required to induce interferon-gamma (IFNγ) expression and to enhance their cytotoxic activity. In conclusion , our findings may imply a direct role of hepatocytes in NK cell development, and the system we have used may provide a tool for studying the molecular mechanisms of NK cell differentiation. (Hepatology 2004;39:1508-1516.)
Reversal of pulmonary vascular remodelling following hypoxic exposure: no effect of infusion of atrial natriuretic factor and neutral endopeptidase inhibitor
Authors: L. Zhao, J M B Hughes, R. J D Winter
Journal: Cardiovascular Research (1994)

Journal Article Reversal of pulmonary vascular remodelling following hypoxic exposure: no effect of infusion of atrial natriuretic factor and neutral endopeptidase inhibitor Get access Lan Zhao, Lan Zhao Department of Medicine (Respiratory Division), Royal Postgraduate Medical School, Hammersmith Hospital, London W12 0NN, United Kingdom: L Zhao, J M B Hughes, R J D Winter. Correspondence to Dr Zhao. Search for other works by this author on: Oxford Academic PubMed Google Scholar J M B Hughes, J M B Hughes Department of Medicine (Respiratory Division), Royal Postgraduate Medical School, Hammersmith Hospital, London W12 0NN, United Kingdom: L Zhao, J M B Hughes, R J D Winter. Search for other works by this author on: Oxford Academic PubMed Google Scholar Robert J D Winter Robert J D Winter Department of Medicine (Respiratory Division), Royal Postgraduate Medical School, Hammersmith Hospital, London W12 0NN, United Kingdom: L Zhao, J M B Hughes, R J D Winter. Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 28, Issue 4, April 1994, Pages 519–523, https://doi.org/10.1093/cvr/28.4.519 Published: 01 April 1994 Article history Received: 08 July 1993 Accepted: 16 November 1993 Published: 01 April 1994
Effect on Neonatal Jaundice of Oestrogens and Progestogens Taken before and after Conception
Authors: J. B. McConnell, J. F. T. Glasgow, R. McNair
Journal: BMJ (1973)

Study of 182 newborn babies has shown that among bottle-fed infants the mean plasma bilirubin concentration was slightly but significantly higher (P <0.01) in those whose mothers had previously used steroid contraceptives. A similar finding was not noted among breast-fed infants.A further 16 infants whose mothers had received progestogen therapy during their pregnancy had a mean plasma bilirubin concentration which was significantly higher than each of the four other groups of infants studied (P <0.01). Icterus occasionally reached clinically important levels in these babies.