tbuckley/cabot-search huggingface dataset:
Abstract 3197: Gedunin inactivates the co-chaperone p23 causing cancer cell death by apoptosis
Authors: Chaitanya A. Patwardhan, Laura B. Peterson, Brian Blagg, Ahmed Chadli
Journal: Cancer Research (2014)

Abstract Pharmacological inhibition of Hsp90 is an exciting option for cancer therapy. The clinical efficacy of Hsp90 inhibitors is, however, less than expected. Binding of the co-chaperone p23 to Hsp90, and induced overexpression of anti-apoptotic proteins, Hsp70 and Hsp27, are thought to contribute to this outcome. Herein, we report that the natural product, gedunin, may provide a new alternative to inactivate the Hsp90 machine. We show that gedunin directly binds to p23 and inactivates it, without overexpression of Hsp27 and relatively modest induction of Hsp70. Using molecular docking and mutational analysis, we mapped the gedunin-binding site on p23. Functional analysis shows that gedunin inhibits p23 chaperoning activity, blocks its cellular interaction with Hsp90 and interferes with p23-mediated gene regulation. Cell treatment with gedunin leads to cancer cell death by apoptosis through inactivation of p23 and activation of caspase 7, which cleaves p23 at the C-terminus. These results provide important insight into the molecular mechanism of action of this promising lead compound. Citation Format: Chaitanya A. Patwardhan, Laura B. Peterson, Brian Blagg, Ahmed Chadli. Gedunin inactivates the co-chaperone p23 causing cancer cell death by apoptosis. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3197. doi:10.1158/1538-7445.AM2014-3197
Anatomical and Functional Evaluation of Pulmonary Veins in Children by Magnetic Resonance Imaging
Authors: Lars Grosse-Wortmann, Abdulmajeed Al-Otay, Hyun Woo Goo, Christopher K. Macgowan, John G. Coles, Leland N. Benson, Andrew N. Redington, Shi-Joon Yoo
Journal: Journal of the American College of Cardiology (2007)
Long term survival after treatment of disseminated T cell lymphoma presenting with tracheal obstruction in a patient with coeliac disease.
Authors: D J Seddon, K F Chung, F J Paradinas, E S Newlands, P D Snashall
Journal: Thorax (1989)

A 46 year old man with coeliac disease and upper airway obstruction was thought to have undifferentiated carcinoma of the trachea. Subsequent immunohistological examination showed that the neoplasm was a T cell lymphoma. He had combination chemotherapy and is alive and well seven years after diagnosis.
A PROSPECTIVE ANALYSIS OF ATRIAL FIBRILLATION EPISODE NUMBER AND DURATION AFTER PULMONARY VEIN ISOLATION: FINDINGS FROM 12 MONTHS OF CONTINUOUS ECG LOOP RECORDINGS
Authors: Evgeny Pokushalov, Aleksandr Romanov, Sergey Artemenko, Alex Turov, Vitaliy Shabanov, Vera Selina, Aleksandr Karaskov, Giorgio Corbucci
Journal: Journal of the American College of Cardiology (2011)
THE ACUTE TOXICITY OF MERCURIAL DIURETICS
Authors: ARTHUR C. DeGRAFF
Journal: JAMA (1942)

Several human fatalities have recently been reported for which the intravenous injection of a mercurial diuretic was believed to be the causative agent.<sup>1</sup>It therefore seemed desirable to make a comparison of toxicity of the mercurial diuretics by this route and to study the influence of the drugs commonly used in the treatment of heart disease on this toxicity. The drugs used for the comparison were esidrone, mercupurin and salyrgan-theophylline and the three parent mercurials, esidrone without theophylline, mercurin and salyrgan. <h3>PROCEDURE AND RESULTS</h3><h3>Comparative Acute Lethal Dose.—</h3> The cat was selected as the experimental animal. The drugs were injected without dilution so that the concentration of mercury was approximately 40 mg. per cubic centimeter in every case. Injections were given into the femoral vein by means of a micro buret connected to a 19 gage needle with a short piece of rubber catheter.No anesthesia was used and
A False-Positive Hepatobiliary Scan in a Patient with Cystic Fibrosis
Authors: JOHN I. LANE, DAVID SACKS, RANDALL S. WINN, MARILYN S. KERSHNER
Journal: Clinical Nuclear Medicine (1988)

A case of nonvisualization of the gallbladder in an adult patient with cystic fibrosis in the absence of acute cholecystitis is reported. Delayed images to 20 hours showed persistent nonvisualization. Review of the literature on cystic fibrosis suggests that nonvisualization of the gallbladder may be secondary to inspissated mucus rather than acute cholecystitis, and therefore a positive hepatobiliary scan in these patients should be interpreted with caution.
"FALLACIES OF THE 'YEAST METHOD' OF DETECTING GLUCOSE IN THE URINE"
Authors: Aldo Castellani
Journal: JAMA (1926)

<h3>To the Editor:</h3> —The ordinary yeast method may tell us that the urine contains a fermentable substance; the new mycologic method tells us this and in addition tells us what the fermentable substance [ill] Dr. Einhorn states that the type of sugar present is of secondary importance. With this statement I am unable to agree. It seems to me that also from a practical point of view it is of importance to differentiate between glucose, maltose, galactose, lactose and pentose, and a method that enables us to do this in a fairly easy and rapid manner is perhaps of some utility.
Dual Antiplatelet Therapy and Heparin “Bridging” Significantly Increase the Risk of Bleeding Complications After Pacemaker or Implantable Cardioverter-Defibrillator Device Implantation
Authors: Christine Tompkins, Alan Cheng, Darshan Dalal, Jeffrey A. Brinker, Charles T. Leng, Joseph E. Marine, Saman Nazarian, David D. Spragg, Sunil Sinha, Henry Halperin, Gordon F. Tomaselli, Ronald D. Berger, Hugh Calkins, Charles A. Henrikson
Journal: Journal of the American College of Cardiology (2010)
Prognostic Role of Plasma Insulin-Like Growth Factor (IGF) and IGF-Binding Protein 3 in Metastatic Colorectal Cancer
Authors: Xavier Garcia-Albeniz, Rosa Gallego
Journal: Clinical Cancer Research (2009)

To the Editors: Fuchs et al. ([1][1]) report convincing evidence documenting the predictive and prognostic capacity of a single baseline determination of insulin-like growth factor-binding protein 3 (IGFBP3) and IGF-I plasma levels in advanced colorectal cancer patients undergoing first-line
FRACTURE OF THE SPINE OF THE TIBIA
Authors: RICHMOND STEPHENS
Journal: JAMA (1923)

The condition with which we are concerned in this paper is one of rather extreme rarity. In reviewing the literature, it is difficult to find very much written on the subject. In all probability, the injury occurs more frequently than the records would indicate, as in the last six years I have seen two cases.  REPORT OF CASES  Case 1.  —In the fall of 1917, at Base Hospital No. 9, American Expeditionary Forces, a patient was brought in with a severe knee injury. He was about 19 years old, and while playing football was tackled round the knees and thrown to the ground. He experienced a severe twist in his knee as his body lunged, and when he attempted to get up the knee was found to be severely injured. Several hours after the accident he came under our observation, and at this time presented an extremely painful, swollen and
Spurious Elevation of Urinary Catecholamines During Therapy with Alpha-Methyl-Dopa
Authors: Ray W. Gifford
Journal: JAMA (1962)

REFINEMENTS in techniques for the diagnosis of various forms of secondary hypertension and the introduction of effective hypotensive drugs have brightened the outlook for the hypertensive patient, but have compounded the problems of the physician. He must become increasingly aware of and wary of the possibility that the drugs which he is administering may spuriously influence the results of clinical tests on which the decision for surgical intervention may depend. For example, therapy with thiazide diuretics is frequently accompanied by hypokalemia that may be profound enough to cause the physician to suspect primary aldosteronism. Low sodium diets, sometimes employed in the treatment of hypertension, can cause an increase in the urinary excretion of aldosterone and, in certain circumstances, this too might lead to the erroneous diagnosis of primary aldosteronism. It has been shown that treatment with most of the antihypertensive drugs presently available may cause falsely positive or falsely negative
An Enzymatic Basis for Variation in Response to Allopurinol
Authors: William N. Kelley, Frederick M. Rosenbloom, John Miller, J. Edwin Seegmiller
Journal: New England Journal of Medicine (1968)

Although allopurinol appears to decrease the rate of de novo purine biosynthesis, this effect is variable in gout, possibly because uric acid production is increased in patients who are relatively deficient in hypoxanthine-guanine phosphoribosyltransferase (HG-PRTase). To test this possibility, allopurinol was given to 11 patients with excessive uric acid production. In seven allopurinol had no effect on total excretion of purine catabolites. The same seven had a marked decrease in the activity of HG-PRTase and were also resistant to the effects of allopurinol on purine synthesis. These observations suggest that HG-PRTase activity is necessary for allopurinol to suppress purine synthesis de novo in man. This effect of allopurinol was also absent in four additional gouty patients with normal uric acid production and normal HG-PRTase activity. Hence, deficiency of this enzyme does not account for all cases of resistance to suppression of de novo purine biosynthesis by allopurinol.
Randomized Controlled Trial of Fluticasone in Preschool Children with Intermittent Wheeze
Authors: Caroline S. Pao, Sheila A. McKenzie
Journal: American Journal of Respiratory and Critical Care Medicine (2002)

Section:ChooseTop of pageAbstract <<PURPOSE OF STUDYMETHODSRESULTSDISCUSSIONReferencesCITING ARTICLES
Bone regeneration in a massive rat femur defect through endochondral ossification achieved with chondrogenically differentiated MSCs in a degradable scaffold
Authors: Noriko Harada, Yoshinobu Watanabe, Kenji Sato, Satoshi Abe, Katsuyuki Yamanaka, Yuhiro Sakai, Tadashi Kaneko, Takashi Matsushita
Journal: Biomaterials (2014)
Abstract 5205: CCND1 is a miR-193b target in prostate cancer
Authors: Kirsi M. Tuppurainen, Hanna E. Rauhala, Mauro Scaravilli, Robert L. Vessella, Tapio Visakorpi
Journal: Cancer Research (2014)

Abstract Prostate cancer is the most frequent cancer of men in Western countries and causes second most of the cancer-related deaths. In addition to genetic changes also epigenetic alterations and micro-RNAs (miRNAs) are important in prostate cancer development. Our group has previously shown that miR-193b is hypermethylated in 22Rv1 prostate cancer cell line and that it is putative tumor suppressor in prostate cancer. In melanoma and hepatocellular carcinoma, it has been shown that CCND1, encoding cyclin D1 protein, is among the target genes of miR-193b. The aim of this project was to study if CCND1 is a target of miR-193b in prostate cancer. According to qRT-PCR and microarray analyses, the expression of CCND1 and miR-193b were inversely correlated in prostate cancer cell lines (PC-3, VCaP, 22Rv1, LAPC4, DU145, LNCaP) and in LuCaP xenografts. Using a luciferase reporter gene assay in 22Rv1 cells, we showed that miR-193b targets CCND1 3′UTR. In 22Rv1 cells with low endogenous miR-193b expression, transient pre-miR-193b transfection reduced CCND1 mRNA levels and cyclin D1 and phospho-RB protein levels. By rescue experiment we confirmed that the downregulation of cyclin D1 and phospho-RB proteins is due to miR-193b. When prostate cancer cell lines were treated with cdk4/6 inhibitor, 22Rv1 and VCaP cells with low miR-193b and high CCND1 expression showed significant growth retardation. In contrast, the inhibitor did not have any effect on the growth of PC-3 and DU145 cells with high miR-193b and low CCND1 expression. Furthermore, immunohistochemical analysis of 337 specimens showed that cyclin D1 is expressed significantly higher in castration resistant compared to hormone-naïve prostate cancers. Therefore, we conclude that CCND1 is one of the miR-193b targets in prostate cancer. Citation Format: Kirsi M. Tuppurainen, Hanna E. Rauhala, Mauro Scaravilli, Robert L. Vessella, Tapio Visakorpi. CCND1 is a miR-193b target in prostate cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5205. doi:10.1158/1538-7445.AM2014-5205
Journal of Pain | Pain Medicine
Authors: John C. Rowlingson, Kelly Near, Karen Grandage
Journal: JAMA (2003)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Mayo Adhesive Probability Score: An Accurate Image-based Scoring System to Predict Adherent Perinephric Fat in Partial Nephrectomy
Authors: Andrew J. Davidiuk, Alexander S. Parker, Colleen S. Thomas, Bradley C. Leibovich, Erik P. Castle, Michael G. Heckman, Kaitlynn Custer, David D. Thiel
Journal: European Urology (2014)
Loss of <i>p53</i> and <i>Ink4a/Arf</i> Cooperate in a Cell Autonomous Fashion to Induce Metastasis of Hepatocellular Carcinoma Cells
Authors: Ya-Wen Chen, David S. Klimstra, Michelle E. Mongeau, Jessica L. Tatem, Victor Boyartchuk, Brian C. Lewis
Journal: Cancer Research (2007)

Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide. HCC patients frequently present with disease that has metastasized to other regions of the liver, the portal vein, lymph nodes, or lungs, leading to poor prognoses. Therefore, model systems that allow exploration of the molecular mechanisms underlying metastasis in this disease are greatly needed. We describe here a metastatic HCC model generated after the somatic introduction of the mouse polyoma virus middle T antigen to mice with liver-specific deletion of the Trp53 tumor suppressor locus and show the cell autonomous effect of p53 loss of function on HCC metastasis. We additionally find that cholangiocarcinoma also develops in these mice, and some tumors display features of both HCC and cholangiocarcinoma, suggestive of origin from liver progenitor cells. Concomitant loss of the Ink4a/Arf tumor suppressor locus accelerates tumor formation and metastasis, suggesting potential roles for the p16 and p19 tumor suppressors in this process. Significantly, tumor cell lines isolated from tumors lacking both Trp53 and Ink4a/Arf display enhanced invasion activity in vitro relative to those lacking Trp53 alone. Thus, our data illustrate a new model system amenable for the analysis of HCC metastasis.
Incidental, Early Diagnosis Of Urinoma By F-18 FDG PET/CT
Authors: Mar??a J. Sanchez, Vicente J. Caride
Journal: Clinical Nuclear Medicine (2005)

An F-18 FDG positron emission tomography/computed tomography (PET/CT) scan was performed for staging purposes in a 51-year-old asymptomatic man 3 weeks after hand-assisted laparoscopic surgery (HALS) for colorectal cancer. The whole-body F-18 FDG PET/CT demonstrated a left retroperitoneal hyperintense focus of F-18 FDG. The degree of radioactivity was comparable with the bladder, suggesting a urine collection. A CT/IVP obtained after the PET/CT study found a left hydronephrosis and a dilated ureter to the visible level of the bilobed fluid mass. The urinoma was subsequently drained percutaneously. Follow up with CT scan of the abdomen and pelvis showed no reaccumulation of urine. A PET/CT performed 5 months later was negative for retroperitoneal reaccumulation of urine. With PET alone, a hypermetabolic retroperitoneal focus of FDG may have been interpreted as lymphadenopathy if a contemporaneous CT was not available. The integrated PET and CT displayed the structural and functional aspects of the fluid collection, correctly indicating a urine collection.
GRANULAR DEPOSITS OF IMMUNOGLOBULINS IN THE SKIN OF PATIENTS WITH DERMATITIS HERPETIFORMIS. AN IMMUNOFLUORESCENT STUDY
Authors: J. B. MEER
Journal: British Journal of Dermatology (1969)

SUMMARY.— Direct immunofluorescent technique applied to skin sections revealed the occurrence of granular deposits of immunoglobulins in the basement membrane zone of the uninvolved skin from 10 of 12 patients with dermatitis herpetiformis, whereas sections from all 12 patients with pemphigoid yielded a homogeneous immunofluorescent staining pattern. By indirect immunofluorescent technique "basement membrane antibodies" could be demonstrated in the sera from only 7 of 12 patients with pemphigoid. The implications of the in vivo occurrence of immunoglobulins with regard to the pathogenesis of dermatitis herpetiformis and the possibilities for diagnostic applications of the presented findings are discussed.
Management of blood loss in Jehovah's Witnesses
Authors: D. Busuttil, A. Copplestone
Journal: BMJ (1995)

Recombinant human erythropoietin helps but is expensive

The Jehovah's Witness religion was founded in the late 1870s by Charles Russell in Pittsburgh, Pennsylvania. There are 5 million Jehovah's Witnesses worldwide and 125000 in the United Kingdom. Members of this sect do not accept blood transfusions, a stand based on passages from the Bible, such as this from Leviticus: (xvii) “As for any man who eats any sort of blood, I shall certainly set my face against the soul that is eating the blood, and I shall indeed cut him off from among his people.” Blood transfusion is interpreted as the eating of blood, and Jehovah's Witnesses believe that all hope of eternal life will be forfeited if transfusion is accepted. Autologous transfusion is also prohibited because they believe that once blood has left the body, it is then unclean. These prohibitions present ethical and clinical challenges to doctors who look after seriously ill or injured Jehovah's Witnesses and prompt a search for alternatives to blood.

The courts have consistently upheld Jehovah's Witnesses' decisions to refuse transfusions on the grounds that any adult of sound mind has the right to determine what shall be done with his or her own body. Nevertheless, two thirds of European doctors working in intensive care units would give transfusions to an unconscious Jehovah's Witness …
Primary Aortoduodenal Fistula
Authors: Rolf L. Schapiro
Journal: JAMA (1976)

SPONTANEOUS or primary aortoenteric fistula is an uncommon aortomality. It has a considerably greater incidence as a complication of reconstructive aortic surgery. The first report of successful surgical salvage of a patient with primary aortoduodenal fistula appeared in 1961.<sup>1</sup>Preservation of the life of patients with either primary or secondary aortoduodenal fistula depends on prompt diagnosis and treatment.<sup>2-6</sup>The increasing incidence and possibility of successful surgical treatment suggest that this abnormality can no longer be considered a rare clinical curiosity with an obligatory fatal outcome. While the various roentgenographic manifestations of abdominal aortic aneurysm have been reviewed in the literature,<sup>7</sup>there apparently are no reports of the roentgenographic appearance of aortoduodenal fistula on gastrointestinal examinations. We have had the opportunity to perform a gastrointestinal examination on two patients with primary aortoduodenal fistula. Unfortunately, of the improbability of occurrence of primary fistula prevented the diagnosis, although it was
Duplication and Selection on Abalone Sperm Lysin in an Allopatric Population
Authors: N. L. Clark, G. D. Findlay, X. Yi, M. J. MacCoss, W. J. Swanson
Journal: Molecular Biology and Evolution (2007)

While gene duplication is a major source of evolutionary novelty, the importance of this process in reproductive protein evolution has not been widely investigated. Here, we report the first known case of gene duplication of abalone sperm lysin in an allopatric subspecies found in the Eastern Atlantic, Haliotis tuberculata coccinea. Mass spectrometry identified both copies of the lysin protein in testis tissue, and 3-dimensional structural modeling suggests that both proteins remain functional. We also detected positive selection acting on both paralogs after duplication and found evidence of a recent selective sweep. Because H. t. coccinea occurs in geographic isolation from other abalone species, these findings suggest that the evolution of lysin is not driven to create reproductive barriers to unfit hybrid formation with an overlapping species. Instead, sexual selection or sexual conflict acting during abalone fertilization could be responsible for the recent positive selection on this protein. The presence of multiple, rapidly evolving lysin genes in H. tuberculata presents an opportunity to study the early stages of diversification of a protein whose function is well understood.
Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development
Authors: Fu-Jung Lin, Xinpu Chen, Jun Qin, Young-Kwon Hong, Ming-Jer Tsai, Sophia Y. Tsai
Journal: Journal of Clinical Investigation (2010)

The lymphatic system plays a key role in tissue fluid homeostasis. Lymphatic dysfunction contributes to the pathogenesis of many human diseases, including lymphedema and tumor metastasis. However, the mechanisms regulating lymphangiogenesis remain largely unknown. Here, we show that COUP-TFII (also known as Nr2f2), an orphan member of the nuclear receptor superfamily, mediates both developmental and pathological lymphangiogenesis in mice. Conditional ablation of COUP-TFII at an early embryonic stage resulted in failed formation of pre-lymphatic ECs (pre-LECs) and lymphatic vessels. COUP-TFII deficiency at a late developmental stage resulted in loss of LEC identity, gain of blood EC fate, and impaired lymphatic vessel sprouting. siRNA-mediated downregulation of COUP-TFII in cultured primary human LECs demonstrated that the maintenance of lymphatic identity and VEGF-C-induced lymphangiogenic activity, including cell proliferation and migration, are COUP-TFII-dependent and cell-autonomous processes. COUP-TFII enhanced the pro-lymphangiogenic actions of VEGF-C, at least in part by directly stimulating expression of neuropilin-2, a coreceptor for VEGF-C. In addition, COUP-TFII inactivation in a mammary gland mouse tumor model resulted in inhibition of tumor lymphangiogenesis, suggesting that COUP-TFII also regulates neo-lymphangiogenesis in the adult. Thus, COUP-TFII is a critical factor that controls lymphangiogenesis in embryonic development and tumorigenesis in adults.