tbuckley/cabot-search huggingface dataset:
917. GM-CSF Gene Transfer to Acute Limb Ischemic Mouse for Limb Recuperation
Authors: Mariana Grings, Chester B. Sacramento, José C. Baptista-Silva, Christina M. Takiya, Abram Beutel, Cassia T. Bergamaschi, Ruy R. Campos, Jane Z. Moraes, Sang W. Han
Journal: Molecular Therapy (2006)

Introduction Peripheral arterial diseases may be caused by any occlusive leg artery lesion that interferes with blood flow. When these conditions occur, there is an increase in vessel resistance that can lead to a reduction in distal perfusion pressure and blood flow. This process forces collateral vessel remodeling and it is known as arteriogenesis. Inflammation is the main triggering factor of arteriogenesis and several molecular factors are involved. One way to prolong the arteriogenic activity is extending monocytes half-life by apoptosis inhibition with GM-CSF (granulocyte and macrophage colony stimulating factor). This factor is also responsible for proliferation and differentiation of inflammatory cells, which are important growth factors producers and chemoattractants for arteriogenesis. The main objective of this study was to assess the capacity of reverting surgery-induced acute limb ischemia in mice by in vivo transference of plasmid vector with GM-CSF gene and to compare with the VEGF gene transfer. Materials and Methods Two plasmid vectors were used in this study: uP-GM and uP- VEGF to express GM-CSF and VEGF, respectively. To induce acute limb ischemia in mice BALB/c, the femoral artery was excised from its origin of the external iliac artery to the distal point, where it bifurcates into saphenous and popliteal arteries, without damaging the vein and femoral nerve. All branches of femoral artery were ligated. Gene therapy was performed by injecting 100 μg of plasmid vector at the thigh soon after the ischemia surgery. Results and Discussion About 30 % of animals that underwent ischemic surgery lost their limbs after 1 week, meanwhile none of uP-GM treated animals lost their limbs, even after 4 weeks of the surgery. Functional tests of the GM-CSF gene treated muscles revealed about 70 % of recovery of force, meanwhile the VEGF gene treated animals and the non- treated animals recovered only 25 % and 5 % of force respectively after 4 weeks of the gene transfer. Weight of gastrocnemius reached 100 % of recovery with ischemic animals treated with uP-GM after 4 weeks in comparison to the non-ischemic animals, but the VEGF gene treated or non-treated animals showed recovery of only 60 %. Capillary density had a significant increase in the VEGF treated animals after 1 week of surgery, but those treated with GM-CSF did not change in comparison to the non-treated ischemic animals, and this profile continued for 4 weeks. Histological data from the animals treated with GM-CSF for 4 weeks revealed that the muscle organization returned to the normal form. Therefore, we conclude that GM-CSF gene transference therapy in the ischemic muscle promoted good recovery of physical and physiological parameters in the long term meanwhile the use of VEGF gene promoted rapid recovery but it was not sustainable for 4 weeks.
799 EFFICACY AND SAFETY OF TRIPLE-THERAPY WITH PEGINTERFERON-RIBAVIRIN, AND BOCEPREVIR AS COMPASSIONATE-USE IN SPANISH PATIENTS WITH HEPATITIS C GENOTYPE 1 WITH SEVERE FIBROSIS: INTERIM-ANALYSIS AT 12 WEEKS
Authors: J.L. Calleja, J.M. Pascasio, B. Ruiz-Antoran, J.R. Larrubia, F. Gea, J. de la Revilla, X. Forns, R. Barcena, M. Romero-Gomez, J. Crespo, R. Solá, C. Fernández, I. Fernández, M. Buti
Journal: Journal of Hepatology (2013)
Metabolic Disturbances And Seizures
Authors: Micheline Maccario
Journal: JAMA (1972)

<h3>To the Editor.—</h3> I read with interest the case of "Brain Tumor With Hyperosmolar Hyperglycemic Nonketotic Diabetic Coma," reported by Denton et al (218:256, 1971). Interpretation of the sequence of events does not appear logical in the light of what is known about this metabolic disturbance. The authors stated that "the syndrome was precipitated by a seizure related to the meningioma." We described focal seizures as a manifestation of nonketotic hyperglycemia<sup>1</sup>and demonstrated in an experimental model that seizure activity can be stimulated from a previously established epileptogenic focus by the administration of hypertonic glucose solutions.<sup>2</sup>This model is the experimental replica of the clinical situation encountered by Denton et al. It would seem more likely that the seizure the authors report resulted from the combination of a focal lesion rendered epileptogenic by the occurrence of the metabolic imbalance rather than being the cause of the latter. The
Functional Characterization of Atrial Electrograms in Sinus Rhythm Delineates Sites of Parasympathetic Innervation in Patients With Paroxysmal Atrial Fibrillation
Authors: Nicolas Lellouche, Eric Buch, Andrew Celigoj, Carin Siegerman, David Cesario, Carlos De Diego, Aman Mahajan, Noel G. Boyle, Isaac Wiener, Alan Garfinkel, Kalyanam Shivkumar
Journal: Journal of the American College of Cardiology (2007)
Artificial ventilation for neurological disease: retrospective analysis 1972-81.
Authors: J G Douglas, R J Fergusson, G K Crompton, I W Grant
Journal: BMJ (1983)

During the 10 year period 1972-81, 39 patients with neurological disorders referred to a respiratory unit required artificial ventilation, seven on two occasions. The decision to undertake ventilation was usually made on clinical grounds because of deteriorating respiratory effort, ineffective cough, or inability to swallow. Arterial blood gas studies were of limited value in assessing the need for ventilation. The most frequent complication was bronchopulmonary infection which occurred in almost every patient, Staphylococcus pyogenes, Pseudomonas pyocyanea, and coliforms being the organisms most commonly isolated. In contrast, serious complications of tracheostomy and pulmonary thromboembolism occurred infrequently. There were 10 deaths among the 39 patients ventilated on 46 occasions; six were directly attributable to the neurological disease itself but four resulted from complications of artificial ventilation.
Cardiac Nucleotides and Derivatives in Acute and Chronic Ventricular Failure of the Dog Heart
Authors: NANCY M. BUCKLEY, KENNETH K. TSUBOI
Journal: Circulation Research (1961)

The levels and proportionate distribution of nucleotides, nucleosides, and bases were determined in myocardial samples from two freshly excised hearts, three acutely failing left ventricle preparations in which the nonworking right ventricle served as control, and two hearts from dogs in which aortic regurgitation was produced surgically as a source of chronic overload of the left ventricle. Ventricular performance was evaluated from cardiac function studies, including measurements of cardiac output, stroke work, and contractility. The compounds identified by chromatographic resolutions were AMP, ADP, ATP, DPN, UPP, and GPP; inosine and hypoxanthine; and uridine and uracil. Qnantitative analyses revealed no consistent difference in specific composition of nonfailing and acutely or chronically failing ventricles.
Monitoring bile acid transport in single living cells using a genetically encoded Förster resonance energy transfer sensor
Authors: Lieke M. van der Velden, Misha V. Golynskiy, Ingrid T. G. W. Bijsmans, Saskia W. C. van Mil, Leo W. J. Klomp, Maarten Merkx, Stan F.J. van de Graaf
Journal: Hepatology (2012)

Bile acids are pivotal for the absorption of dietary lipids and vitamins and function as important signaling molecules in metabolism. Here, we describe a genetically encoded fluorescent bile acid sensor (BAS) that allows for spatiotemporal monitoring of bile acid transport in single living cells. Changes in concentration of multiple physiological and pathophysiological bile acid species were detected as robust changes in Förster resonance energy transfer (FRET) in a range of cell types. Specific subcellular targeting of the sensor demonstrated rapid influx of bile acids into the cytoplasm and nucleus, but no FRET changes were observed in the peroxisomes. Furthermore, expression of the liver fatty acid binding protein reduced the availability of bile acids in the nucleus. The sensor allows for single cell visualization of uptake and accumulation of conjugated bile acids, mediated by the Na+-taurocholate cotransporting protein (NTCP). In addition, cyprinol sulphate uptake, mediated by the putative zebrafish homologue of the apical sodium bile acid transporter, was visualized using a sensor based on the zebrafish farnesoid X receptor. The reversible nature of the sensor also enabled measurements of bile acid efflux in living cells, and expression of the organic solute transporter αβ (OSTαβ) resulted in influx and efflux of conjugated chenodeoxycholic acid. Finally, combined visualization of bile acid uptake and fluorescent labeling of several NTCP variants indicated that the sensor can also be used to study the functional effect of patient mutations in genes affecting bile acid homeostasis. Conclusion: A genetically encoded fluorescent BAS was developed that allows intracellular imaging of bile acid homeostasis in single living cells in real time.
INFERIOR VENA CAVA MEASUREMENT BY FOCUSED CARDIAC ULTRASOUND IN ACUTE DECOMPENSATED HEART FAILURE PREVENTS HOSPITAL READMISSIONS
Authors: Luke J. Laffin, Amit Patel, Narayan Saha, Julian Barbat, James K. Hall, Matthew Cain, Kishan Parikh, Jay Shah, Kirk Spencer
Journal: Journal of the American College of Cardiology (2014)
Le Médecine de l'Amerique Précolombienne
Authors: Lester S. King
Journal: JAMA (1970)

In the field of medical history probably the most beautiful books published today are the volumes of Les Éditions Roger Dacosta. Several of their books, dealing for example with ancient and modern surgery (up to the 18th century) or gynecological disorders, have been reviewed in these pages in the last few years. The latest volume in their series deals with precolumbian medicine. The author, professor of the history of medicine (as well as surgery) at the University of Paris, covers Mexico, Central America, South America, and includes some material on the North American Indians. But the greater part of the book has to do with Aztec, Mayan, and Inca culture. The available sources fall into several categories. There are the artefacts—the pottery, statuettes, figurines, carvings, and other art works. Of crucial value is the study of the human remains, from archeological excavations, and the whole broad field of paleopathology. Then,
A NEW URINE COLLECTION TUBE HOLDER
Authors: Edward William White
Journal: JAMA (1930)

In this communication I am presenting to the profession a practical and inexpensive device to be used during the act of ureteral catheterization and separate urine collections. The holder is an especially designed stand or retort made of materials sufficiently strong to insure long life and frequent sterilization. The test tube clips are constructed of well tempered spring steel which will firmly grasp any size collecting container. A set screw is conveniently placed in order to fix the apparatus to the waste receptacle of the cytoscopic table, hence avoiding the danger of upsetting or contaminating the urine collection. I have found after considerable experimentation that the new holder is indeed helpful and practical.
Strain-specific activation of the NF-κB pathway by GRA15, a novel <i>Toxoplasma gondii</i> dense granule protein
Authors: Emily E. Rosowski, Diana Lu, Lindsay Julien, Lauren Rodda, Rogier A. Gaiser, Kirk D.C. Jensen, Jeroen P.J. Saeij
Journal: The Journal of Experimental Medicine (2011)

NF-κB is an integral component of the immune response to Toxoplasma gondii. Although evidence exists that T. gondii can directly modulate the NF-κB pathway, the parasite-derived effectors involved are unknown. We determined that type II strains of T. gondii activate more NF-κB than type I or type III strains, and using forward genetics we found that this difference is a result of the polymorphic protein GRA15, a novel dense granule protein which T. gondii secretes into the host cell upon invasion. A GRA15-deficient type II strain has a severe defect in both NF-κB nuclear translocation and NF-κB–mediated transcription. Furthermore, human cells expressing type II GRA15 also activate NF-κB, demonstrating that GRA15 alone is sufficient for NF-κB activation. Along with the rhoptry protein ROP16, GRA15 is responsible for a large part of the strain differences in the induction of IL-12 secretion by infected mouse macrophages. In vivo bioluminescent imaging showed that a GRA15-deficient type II strain grows faster compared with wild-type, most likely through its reduced induction of IFN-γ. These results show for the first time that a dense granule protein can modulate host signaling pathways, and dense granule proteins can therefore join rhoptry proteins in T. gondii’s host cell–modifying arsenal.
Resolution of Postvagotomy Dysphagia
Authors: Stephen Lee Carter
Journal: JAMA (1978)

A PATIENT with severe dysphagia following vagotomy and antrectomy led me to review the literature concerning the frequency, cause, and treatment of postvagotomy dysphagia. The patient described illustrates the difficulty encountered in this rare complication and also demonstrates the satisfactory results obtained with conservative management. <h3>Report of a Case</h3> A 32-year-old woman had a year's history of duodenal ulcer confirmed by gastrointestinal (GI) tract roentgenograms and endoscopy. Her gastric analysis showed a basal acid output of 2.1 mEq/hr and a maximum stimulated output of 17.4 mEq/hr. Because of the failure of her medical treatment, she underwent vagotomy and antrectomy with a Billroth II anastomosis. She had an unremarkable postoperative course except for a wound hematoma, possibly due to prophylactic therapy with subcutaneous heparin sodium, 5,000 units every 12 hours. She left the hospital one week postoperatively able to eat soft foods. However, she returned three days after discharge, vomiting, stating
Impact of the Extent of Coronary Artery Disease on Outcomes After Revascularization for Unprotected Left Main Coronary Artery Stenosis
Authors: Young-Hak Kim, Duk-Woo Park, Won-Jang Kim, Jong-Young Lee, Sung-Cheol Yun, Soo-Jin Kang, Seung-Whan Lee, Cheol Whan Lee, Myeong-Ki Hong, Seong-Wook Park, Seung-Jung Park
Journal: Journal of the American College of Cardiology (2010)
A New Electronic Theory of Life (1925)
Authors: J. Stark
Journal: BMJ (2012)

Electrotherapy is most often associated with its modern manifestations, yet it has a tradition that stretches back to at least the mid-18th century. One of the major advocates of this technique was Otto Overbeck (1852-1937), an eccentric industrial scientist and inventor. He studied chemistry at University College London and worked for a brewery in Grimsby as scientific director—a common career path for chemists in the late 19th century. While there, Overbeck patented a number of devices in relation to his work, but he is best remembered for contributions to electrotherapy in his later life.

His health deteriorated during the early 1920s, and he became increasingly desperate in his …
TCT-21 Prognosis of Clinical Outcomes in Chinese Patients Receiving XIENCE V Everolimus-Eluting Stent with Baseline and Residual Syntax Score: One-Year Results from the Prospective Multicenter SEEDS Study
Authors: Bo Xu, Yuejin Yang, Yaling Han, Bao Li, Qiang Liu, Guoying Zhu, Junyu Cui, Lang Li, Yelin Zhao, Shuzheng Lu
Journal: Journal of the American College of Cardiology (2013)
Understanding food allergen thresholds requires careful analysis of the available clinical data
Authors: Steve L. Taylor, Geert F. Houben, Joseph L. Baumert, Rene R.W.R. Crevel, Katrina J. Allen, Anthony E.J. Dubois, Andre C. Knulst, Benjamin C. Remington, Astrid G. Kruizinga, W. Marty Blom, Simon Brooke-Taylor
Journal: Journal of Allergy and Clinical Immunology (2014)
In-111 Ibritumomab Scintigraphy (Planar and SPECT) and FDG PET/CT Before Y-90 Ibritumomab Treatment in a Patient With Mantle Cell Lymphoma
Authors: Julio Francisco Jiménez-Bonilla, Remedios Quirce, Ignacio Banzo, Isabel Martínez-Rodríguez, Aurora Sainz-Esteban, Jorge Enrique Barragán, Juan José López-Cordovilla, José Manuel Carril
Journal: Clinical Nuclear Medicine (2007)

We report the case of a 75-year-old man, in whom Y-90 ibritumomab was requested because of relapse of blastoid variant mantle cell lymphoma diagnosed in 1995. Before Y-90 ibritumomab treatment, FDG PET and In-111 ibritumomab scintigraphy with planar views at 24 hours, 48 hours, and 5 days, including SPECT, were performed. Discordant information between both examinations was observed as, in addition to the lesions detected by In-111 ibritumomab imaging, FDG PET detected lesions that did not take up the ibritumomab. The discrepancy shown by both radiotracers has to be kept in mind before planning treatment with Y-90 ibritumomab, and for the correct evaluation of treatment response.
Undisclosed Conflicts of Interest
Authors: Daniel M. Musher
Journal: Annals of Internal Medicine (2006)

Letters7 February 2006Undisclosed Conflicts of InterestDaniel M. Musher, MDDaniel M. Musher, MDFrom the Baylor College of Medicine, Houston, TX 77005.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-144-3-200602070-00028 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:As an infectious disease specialist, I was surprised that 3 (20%) of the 15 articles that were cited in the recent Update in Cardiology (1) dealt with 1 specific drug, atorvastatin. Dr. Peter H. Stone, the author of the Update, claimed that he had no conflict of interest. However, with the vast number of drug studies and the intense competition in the cardiology field, I found 3 citations on atorvastatin to be remarkably disproportionate.My simple search of PubMed for “PH Stone” and “atorvastatin” yielded 3 articles (2-4). The research for all of these articles was supported ...References1. Stone PH. Update in cardiology. Ann Intern Med. 2005;143:737-43. [PMID: 16287795] LinkGoogle Scholar2. Kinlay S, Timms T, Clark M, Karam C, Bilodeau T, Ridker PM, et al. Comparison of effect of intensive lipid lowering with atorvastatin to less intensive lowering with lovastatin on C-reactive protein in patients with stable angina pectoris and inducible myocardial ischemia. Am J Cardiol. 2002;89:1205-7. [PMID: 12008177] CrossrefMedlineGoogle Scholar3. Stone PH, Lloyd-Jones DM, Johnstone M, Carlson W, Rubenstein J, Creager M, et al. Vascular Basis for the Treatment of Myocardial Ischemia Study: trial design and baseline characteristics. Am Heart J. 2004;147:875-82. [PMID: 15131545] CrossrefMedlineGoogle Scholar4. Stone PH, Lloyd-Jones DM, Kinlay S, Frei B, Carlson W, Rubenstein J, et al. Effect of intensive lipid lowering, with or without antioxidant vitamins, compared with moderate lipid lowering on myocardial ischemia in patients with stable coronary artery disease: the Vascular Basis for the Treatment of Myocardial Ischemia Study. Circulation. 2005;111:1747-55. [PMID: 15809368] CrossrefMedlineGoogle Scholar Author, Article, and Disclosure InformationAffiliations: From the Baylor College of Medicine, Houston, TX 77005.Disclosures: None disclosed. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoUpdate in Cardiology Peter H. Stone Undisclosed Conflicts of Interest Peter H. Stone Metrics Cited byConflicts of interest: Bias or boon? 7 February 2006Volume 144, Issue 3Page: 225KeywordsConflicts of interestDrugsInfectious diseases ePublished: 7 February 2006 Issue Published: 7 February 2006 Copyright & PermissionsCopyright © 2006 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
659 BLEEDING RISK OF ENDOSCOPIC VARICEAL LIGATION (EVL) IN PATIENTS WITH PORTAL VEIN THROMBOSIS (PVT) AND ANTICOAGULATION: AN ANALYSIS OF 1235 PROCEDURES
Authors: C. Christol, A. Plessier, M. Corbic, J.M. Peron, J.P. Vinel, D. Valla, C. Bureau
Journal: Journal of Hepatology (2012)
Alterations of surface receptors on intralesional neutrophils in pustular psoriasis and palmo-plantar pustulosis
Authors: K. IWATSUKI, S. IMAIZUMI, M. TSUGIKI, M. YAMADA, H. TAGAMI
Journal: British Journal of Dermatology (1985)

Journal Article Alterations of surface receptors on intralesional neutrophils in pustular psoriasis and palmo‐plantar pustulosis Get access K. IWATSUKI, K. IWATSUKI Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu, Japan Dr Keiji Iwatsuki, Department of Dermatology, Hamamatsu University School of Medicine, 3600 Handacho, Hamamatsu 431‐31, Japan. Search for other works by this author on: Oxford Academic Google Scholar S. IMAIZUMI, S. IMAIZUMI Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu, Japan Search for other works by this author on: Oxford Academic Google Scholar M. TSUGIKI, M. TSUGIKI Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu, Japan Search for other works by this author on: Oxford Academic Google Scholar M. YAMADA, M. YAMADA Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu, Japan Search for other works by this author on: Oxford Academic Google Scholar H. TAGAMI H. TAGAMI Department of Dermatology, Tohoku University School of Medicine, Sendai, Japan Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 112, Issue 1, 1 January 1985, Pages 53–56, https://doi.org/10.1111/j.1365-2133.1985.tb02290.x Published: 01 January 1985 Article history Accepted: 30 May 1984 Published: 01 January 1985
Phospholipase A and Lysophospholipase activity of the epidermis
Authors: V.J.W. LONG
Journal: British Journal of Dermatology (1975)

Phospholipase A and lysophospholipase activities have been demonstrated in cow snout epidermis. The phospholipase A activity was dependent on Ca2+ ions and the pH for optimum activity was between 6-1 and 7-4.
Epigenetic gene silencing in cancer
Authors: Benjamin Tycko
Journal: Journal of Clinical Investigation (2000)

The classical theory of recessive oncogenesis predicted a mutational mechanism for the inactivation of tumor suppressor (TS) genes. This prediction has been amply confirmed, but an alternative, nonmutational, pathway for loss of TS gene activity has also come into focus. For some TS genes, this epigenetic pathway is more frequent than the mutational one. The best-studied DNA modification that correlates with epigenetic gene silencing is methylation of cytosine residues in CpG sequences, and CpG methylation has recently been linked to an even more general mechanism of epigenetic silencing, histone deacetylation. From a combination of descriptive studies and manipulative experiments, some hints of mechanisms for epigenetic silencing of TS genes in cancer cells are beginning to emerge. Here, I discuss several well-documented examples of epigenetic gene silencing in human cancers. I then consider potential mechanisms for de novo methylation of TS genes in cancer. These include spreading of DNA methylation from repetitive sequences into promoterassociated CpG islands secondary to loss of transcriptional activator proteins, gain of methylation secondary to hyperexpression of transcriptional repressors, primary hypermethylation due to hyperexpression of methyltransferases, and interallelic transfer of methylation via gene pairing. In some cancers, environmental pressures that select for a hypermethylating cellular phenotype may drive these processes. DNA methylation and heritable gene silencing Epigenetic gene silencing refers to nonmutational gene inactivation that can be faithfully propagated from precursor cells to clones of daughter cells. The addition of methyl groups to cytosine residues in CpG dinucleotides in DNA is a biochemical modification that meets this requirement. A very striking example of the potential for CpG methylation to be heritable is provided by epimutations in plants. Genes carrying epimutations cause morphological phenotypes to be transmitted from generation to generation, not based on any alteration in the