tbuckley/cabot-search huggingface dataset:
Abstract 2780: Bortezomib induces autophagy in hepatocellular carcinoma through the inhibition of CIP2A independent of proteasome inhibition.
Authors: Hui-Chuan Yu, Duen-Ren Hou, Chun-Yu Liu, Ann-Lii Cheng, Pei-Jer Chen, Kuen-Feng Chen
Journal: Cancer Research (2012)

Abstract Previously, we have reported that cancerous inhibitor of protein phosphatase 2A (CIP2A) mediates the apoptotic effect of bortezomib in hepatocellular carcinoma (HCC) (Oncogene, 2010). Here, we report the mechanism by which bortezomib induces autophagy in HCC. Our data indicated that bortezomib activated autophagy in a dose- and time- dependent manner in HCC cell lines, including Huh-7, Sk-Hep1, and Hep3B. Bortezomib down-regulated CIP2A, phospho-Akt (P-Akt) and phospho-4EBP1 (P-4EBP1) in a dose- and time- dependent manner in all tested HCC cells. Ectopic expression of CIP2A abolished the effect of bortezomib on autophagy. Cotreatment of bortezomib and calculin A, a PP2A inhibitor, reduced the effect of bortezomib on P-Akt, P-4EBP1, and autophagy. Over-expression of either Akt or 4EBP1 protected cells from bortezomib-induced autophagy. Moreover, we examined the effect of αBtz, a bortezomib derivative that closely resembles bortezomib structurally but with no proteasome activity, in HCC. Interestingly, αBtz demonstrated similar effects to bortezomib on autophagy, CIP2A, P-Akt and P-4EBP1. Furthermore, our in vivo data showed that both bortezomib and αBtz inhibited tumor growth, down-regulated CIP2A, P-Akt and induced autophagy in Huh-7 tumors, suggesting that bortezomib activates autophagy in HCC and this effect is independent to its effect on proteasome. In conclusion, bortezomib induces autophagy in HCC through CIP2A-PP2A-Akt-4EBP1 pathway. Supported by NTUH100P04, NSC99-2314-B-002-017-MY2, NSC100-3112-B-002-013, and NSC100-2325-B-002-036. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2780. doi:1538-7445.AM2012-2780
Abnormal expression and function of Dectin-1 receptor in type 2 diabetes mellitus patients with poor glycemic control (HbA1c &gt; 8%)
Authors: Nancy Cortez-Espinosa, Mariana H. García-Hernández, Elizabeth Reynaga-Hernández, J. Diego Cortés-García, Nancy E. Corral-Fernández, J. Guillermo Rodríguez-Rivera, Anamaría Bravo-Ramírez, Roberto González-Amaro, Diana P. Portales-Pérez
Journal: Metabolism (2012)
The Origins of Human Disease
Authors: William H. McNeill
Journal: JAMA (1989)

"Origins," as used in the title of this book, has two distinct meanings. It may refer to the time, place, and social circumstances under which various kinds of diseases arise among human communities, but it may also mean the circumstances under which diseases become likely to affect individuals. McKeown addresses both of these themes in this posthumous work. Part I, "Disease History" establishes the proposition that human society has passed through three stages—hunting and gathering, agriculture, and industry—and that each stage has its corresponding array of characteristic diseases. Hunters and gatherers suffered mainly from lack of food. Infectious diseases became the predominant causes of sickness and death in the agricultural age. Noncommunicable diseases have taken over pride of place in the age of industry. Throughout this typology, McKeown emphasizes nutrition as the main, ruling factor in human encounters with disease. But his claim that hunger was the main cause of
Allorestricted T cells with specificity for the FMNL1-derived peptide PP2 have potent antitumor activity against hematologic and other malignancies
Authors: Ingrid G. Schuster, Dirk H. Busch, Elfriede Eppinger, Elisabeth Kremmer, Slavoljub Milosevic, Christine Hennard, Christina Kuttler, Joachim W. Ellwart, Bernhard Frankenberger, Elfriede Nößner, Christoph Salat, Christian Bogner, Arndt Borkhardt, Hans-Jochem Kolb, Angela M. Krackhardt
Journal: Blood (2007)
Expression and subcellular distribution of basic fibroblast growth factor are regulated during migration of endothelial cells.
Authors: S Biro, Z X Yu, Y M Fu, G Smale, J Sasse, J Sanchez, V J Ferrans, W Casscells
Journal: Circulation Research (1994)

Migration of endothelial cells is involved in normal and pathological angiogenesis and in re-endothelialization after vascular injury or rupture of atherosclerotic plaques. Several types of endothelial cells are known to synthesize basic fibroblast growth factor (bFGF); in some of these, migration is increased by exogenous bFGF and inhibited by anti-bFGF antibodies. Using immunocytochemical techniques and RNase protection analysis, we studied endothelial cells from bovine coronary arteries and veins as well as from adrenal microvessels. We found that bFGF mRNA and peptide were present in confluent endothelial cells and were upregulated during migration stimulated by removal of some cells from the monolayer. During migration, extracellular matrix stores of bFGF were depleted, and bFGF immunoreactivity began to accumulate in the cytoplasm of endothelial cells between 2 and 6 hours. After migration had begun, but before the initiation of DNA synthesis, bFGF immunoreactivity increased in the nuclei and nucleoli. Exogenous bFGF stimulated endothelial migration, and antibodies to bFGF markedly inhibited migration, suggesting that an intracrine function of nuclear bFGF is not sufficient for cell migration. In all three types of endothelial cells studied, bFGF was identified as an endogenous regulator, but not as the sole regulator, or migration. Moreover, bFGF expression and subcellular localization were found to be regulated during endothelial cell migration.
Editorial de la SEED
Authors: E Vázquez-Sequeiros, E Brullet, JM Bordas
Journal: Endoscopy (2011)

The Spanish Society of Digestive Endoscopy (SEED) is happy to present the abstracts presented to the Spanish Annual Meeting of Endoscopy (Jornada Nacional de la SEED) in this issue. The improvements made by the Board of the Society have been taken into account by the members of the Society, promoting a great increase in the number and quality of the abstracts. In fact, the Meeting has received a total of 249 abstracts including communications, case reports and videos for discussion.
Titanium Microbead‐Based Porous Implants: Bead Size Controls Cell Response and Host Integration
Authors: Nihal Engin Vrana, Agnès Dupret‐Bories, Philippe Schultz, Christian Debry, Dominique Vautier, Philippe Lavalle
Journal: Advanced Healthcare Materials (2013)

Abstract Openly porous structures in implants are desirable for better integration with the host tissue. Sintered microbead‐based titanium implants for oto‐rhinolaryngology applications, which create an environment where the cells can migrate in the areas between the microbeads, are developed. This structure promotes fibrovascular tissue formation within the implant in vivo. In this study, it is determine to what extent these events can be controlled by changing the physical environment of the implants both in vitro and in vivo. By cell tracking, it is observed that the size of the beads and the distance between the neighboring beads significantly affect the ability of cells to develop cell‐to‐cell contacts and to bridge the pores. Live cell staining shows that as the bead size gets smaller, the probability to observe cells that fill the porous areas is higher. This also affects the initial attachment and distribution of the cells and collagen secretion by fibroblasts. Obtaining a fast coverage of the system also enables co‐culture systems where, the number and the distribution of the second cell type are boosted by the presence of the first. This concept is utilized to increase the attachment of vascular endothelial cells by an initial layer of fibroblasts. By decreasing the bead diameter, the overall colonization of the implant can be significantly increased in vivo. The effect of bead size has a similar pattern both in rats and rabbits, with faster colonization of smaller bead‐based structures. Using smaller beads would improve clinical outcomes as faster integration facilitates the attainment of functionality by the implant.
Monocytes control natural killer cell differentiation to effector phenotypes
Authors: Katrina Soderquest, Nick Powell, Carmelo Luci, Nico van Rooijen, Andrés Hidalgo, Frederic Geissmann, Thierry Walzer, Graham M. Lord, Alfonso Martín-Fontecha
Journal: Blood (2011)

Abstract Natural killer (NK) cells play a major role in immunologic surveillance of cancer. Whether NK-cell subsets have specific roles during antitumor responses and what the signals are that drive their terminal maturation remain unclear. Using an in vivo model of tumor immunity, we show here that CD11bhiCD27low NK cells migrate to the tumor site to reject major histocompatibility complex class I negative tumors, a response that is severely impaired in Txb21−/− mice. The phenotypical analysis of Txb21-deficient mice shows that, in the absence of Txb21, NK-cell differentiation is arrested specifically at the CD11bhiCD27hi stage, resulting in the complete absence of terminally differentiated CD11bhiCD27low NK cells. Adoptive transfer experiments and radiation bone marrow chimera reveal that a Txb21+/+ environment rescues the CD11bhiCD27hi to CD11bhiCD27low transition of Txb21−/− NK cells. Furthermore, in vivo depletion of myeloid cells and in vitro coculture experiments demonstrate that spleen monocytes mediate the terminal differentiation of peripheral NK cells in a Txb21- and IL-15Rα–dependent manner. Together, these data reveal a novel, unrecognized role for Txb21 expression in monocytes in promoting NK-cell development and help appreciate how various NK-cell subsets are generated and participate in antitumor immunity.
The Doctor-Activist: Physicians Fighting for Social Change
Authors: James C. Cobey
Journal: JAMA (1996)

This book contains the autobiographies of 10 physicians who have tried to change the world around them. The basic theme is that "doctors care for others." This care may be on a personal therapeutic basis or a larger public health basis, in which physicians use all their energies to change health and living conditions for those around them. The foreword by Julius Richmond, MD, and the first chapter by the editor are possibly the most enjoyable parts of the book. They find the common threads of the ensuing autobiographies: concern for social problems over the business of medicine, inventiveness in interventions in medical and social problems, courage in the face of criticism, and high energy levels. Activist physicians often incur stress in their personal lives in order to fight seemingly unwinnable battles for a common good. Their lives are often not as clearly directed as those of physicians in clinical
Sperm swim singly after vitamin C therapy
Authors: Elizabeth Rasche González
Journal: JAMA (1983)

Swallow a gram of ascorbic acid daily and restore fertility in just four days? It sounds implausible, to say the least. But Earl B. Dawson, PhD—with colleagues William A. Harris and William J. McGanity, MD, Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston—has recently shown that this may be possible in men with infertility secondary to nonspecific sperm agglutination. At the recent meeting of the Federation of American Societies for Experimental Biology in Chicago, Dawson reported on a study of 35 male patients who could not impregnate their wives because more than 20% (mean, 37%) of their sperm clumped together, as demonstrated by microscopic studies of semen samples. The men's serum ascorbic acid levels averaged 0.2 mg/dL, which is considered borderline by the US National Nutrition Research Council, in contrast to a normal level of 0.6 to 0.8 mg/dL. Semen levels of the vitamin averaged 4.2 mg/dL.
Influence of Bedsharing Activity on Breastfeeding Duration Among US Mothers
Authors: Yi Huang, Fern R. Hauck, Caroline Signore, Airong Yu, Tonse N. K. Raju, Terry T.-K. Huang, Sara B. Fein
Journal: JAMA Pediatrics (2013)

Some professional associations advocate bedsharing to facilitate breastfeeding, while others recommend against it to reduce the risk of sudden infant death syndrome and suffocation deaths. A better understanding of the quantitative influence of bedsharing on breastfeeding duration is needed to guide policy.To quantify the influence of bedsharing on breastfeeding duration.Longitudinal data were from the Infant Feeding Practices Study II, which enrolled mothers while pregnant and followed them through the first year of infant life. Questionnaires were sent at infant ages 1 to 7, 9, 10, and 12 months, and 1846 mothers answered at least 1 question regarding bedsharing and were breastfeeding at infant age 2 weeks.Bedsharing, defined as the mother lying down and sleeping with her infant on the same bed or other sleeping surfaces for nighttime sleep or during the major sleep period.Survival analysis to investigate the effect of bedsharing on duration of any and exclusive breastfeeding.Longer duration of bedsharing, indicated by a larger cumulative bedsharing score, was associated with a longer duration of any breastfeeding but not exclusive breastfeeding, after adjusting for covariates. Breastfeeding duration was longer among women who were better educated, were white, had previously breastfed, had planned to breastfeed, and had not returned to work in the first year postpartum.Multiple factors were associated with breastfeeding, including bedsharing. Given the risk of sudden infant death syndrome related to bedsharing, multipronged strategies to promote breastfeeding should be developed and tested.
TRICUSPID ANNULAR PLANE SYSTOLIC EXCURSION: CORRELATION WITH HEMODYNAMIC PARAMETERS IN CHRONIC THROMBOEMBOLIC PULMONARY HYPERTENSION
Authors: Unnati Sampat, Brian Kolski, William R. Auger, Karen McClure, Michael Gibson, Michael M. Madani, George Mesa, Laura Mortensen, Therese Romero, Lori B. Daniels, Daniel G. Blanchard
Journal: Journal of the American College of Cardiology (2011)
Intercapillary distance and capillary reserve in hypertrophied rat hearts beating in situ.
Authors: L Henquell, C L Odoroff, C R Honig
Journal: Circulation Research (1977)

Functional intercapillary distance (ICD) was measured in stop-motion photomicrographs of hypertrophied, normally compensated, well oxygenated rat hearts beating in situ. Left ventricular hypertrophy was produced by salt loading and unilateral nephrectomy. Minimum ICD (when all capillaries are open) also was measured. Ventricular weight increased by 30-40% within 8-9 weeks after nephrectomy. To compare the effect of normal and pathological growth, ICD was also measured in normal rats. In normal animals, minimum ICD and functional ICD increased linearly and proportionately with left ventricular weight. Consequently, the extent to which capillary recruitment could decrease ICD was the same in large and small normal hearts (about 2 micrometer). In the hypertrophied hearts, capillary recruitment could have maintained ICD within normal limits at rest for several weeks. After 8-9 weeks, however, the capillary reserve in hypertrophy was fully utilized at rest, and mean functional ICD was 1.5-2.0 micrometer greater than normal for the age of the animal. An analysis of O2 transport indicates that anoxic foci would exist throughout the hypertrophied heart and particularly in subendocardium when the capillary reserve is exhausted. The calculated amount of anoxic tissue appears sufficient to account for the focal necrosis and fibrosis observed in hypertrophy and for the development of circulatory failure.
Macromolecular Lactic Acid Dehydrogenase
Authors: DAVID W. THOMAS
Journal: Annals of Internal Medicine (1974)

Increased serum lactate dehydrogenase activity (LDH) in a 62-year-old woman, found on routine screening, that persisted for 3 years has been investigated. Gel chromatography showed 99% of the LDH was in a form larger than normal; the remaining 1%, as well as the LDH in saliva and duodenal fluid, was apparently of normal molecular size. Serum protein studies showed increased immunoglobulin A (IgA) concentration, predominantly of the kappa light chain type. Immunoelectrophoretic and immunoprecipitation studies showed that nearly all the LDH was associated with the IgA. Lactic dehydrogenase isoenzyme separation showed an additional band between the normal third and fourth isoenzyme bands, corresponding to the region of IgA migration. The persistently high serum LDH activity may reflect slower clearance of the IgA-bound LDH. Macromolecular LDH must be included in the differential diagnosis of increased serum LDH.
'Ticking Clocks' and Changing Mores
Authors: Chris Anne Raymond
Journal: JAMA (1987)

IN A RECENT BEST-SELLER,<i>The Handmaid's Tale</i>(Atwood M, New York, Simon &amp; Schuster Inc Publishers, 1986), surrogate motherhood was taken to a chilling conclusion—a caste of women whose sole function was to make their wombs available for impregnation by ruling-class men. A medical ethicist and a sociologist have completed a seven-year study of what may be a feminist reversal on this tale—women who seek out a sperm donor in order to have a child before their "biological clocks" cease ticking. Daniel Wikler, PhD, professor of philosophy at the University of Wisconsin, Madison, discovered this practice when his sister, Norma Wikler, PhD, professor of sociology at the University of California, Santa Cruz, was interviewing career women about their decisions on childbearing. One woman admitted she had inseminated herself because she was neither married nor involved in a serious relationship, but wished to have a child. Intrigued, the Wiklers sought out
TCT- 509 Treatment of Acute Pulmonary Embolism with Rheolytic Thrombectomy
Authors: Taida Ivanauskiene, Valdas Bilkis, Giedrius Davidavicius, Michailas Jerdiakovas, Mindaugas Mataciunas, Vytautas Abraitis, Diana Zakarkaite, Sigute Aidietiene, Audrius Aidietis
Journal: Journal of the American College of Cardiology (2014)
Obstructive sleep apnoea with Arnold-Chiari malformation.
Authors: M J Doherty, D P Spence, C Young, P M Calverley
Journal: Thorax (1995)

A case of obstructive sleep apnoea associated with the Arnold-Chiari malformation is described, in which the loss of pharyngeal sensation seems to have played an important part in the aetiology of the obstruction of the upper airway.
Exercise-Induced Bronchospasm and Atopy in Ghana: Two Surveys Ten Years Apart
Authors: Emmanuel O. D Addo-Yobo, Ashley Woodcock, Adorkor Allotey, Benjamin Baffoe-Bonnie, David Strachan, Adnan Custovic
Journal: PLoS Medicine (2007)

Asthma and allergic diseases have increased in the developed countries. It is important to determine whether the same trends are occurring in the developing countries in Africa. We aimed to determine the time trend in the prevalence of exercise-induced bronchospasm (EIB) and atopic sensitisation over a ten-year period in Ghanaian schoolchildren.Two surveys conducted using the same methodology ten years apart (1993 and 2003) among schoolchildren aged 9-16 years attending urban rich (UR), urban poor (UP), and rural (R) schools. Exercise provocation consisted of free running for six minutes. Children were skin tested to mite, cat, and dog allergen. 1,095 children were exercised in 1993 and 1,848 in 2003; 916 were skin tested in 1993 and 1,861 in 2003. The prevalence of EIB increased from 3.1% (95% CI 2.2%-4.3%) to 5.2% (4.3%-6.3%); absolute percentage increase 2.1% (95% CI 0.6%-3.5%, p < 0.01); among UR, UP, and R children EIB had approximately doubled from 4.2%, 1.4%, and 2.2% to 8.3%, 3.0% and 3.9% respectively. The prevalence of sensitisation had also doubled from 10.6%, 4.7%, and 4.4% to 20.2%, 10.3%, and 9.9% (UR, UP, and R respectively). Mite sensitisation remained unchanged (5.6% versus 6.4%), but sensitisation to cat and dog increased considerably from 0.7% and 0.3% to 4.6% and 3.1%, respectively. In the multiple logistic regression analysis, sensitisation (odds ratio [OR] 1.77, 95% CI 1.12-2.81), age (OR 0.88, 95% CI 0.79-0.98), school (the risk being was significantly lower in UP and R schools: OR 0.40, 95% CI 0.23-0.68 and OR 0.54, 95% CI 0.34-0.86, respectively) and year of the study (OR 1.73, 95% CI 1.13-2.66) remained significant and independent associates of EIB.The prevalence of both EIB and sensitisation has approximately doubled over the ten-year period amongst 9- to 16-year-old Ghanaian children irrespective of location, with both EIB and atopy being more common among the UR than the UP and R children.
Not on My Shift
Authors: Lynn A. Crosby
Journal: JAMA (1985)

She was a 72-year-old woman who had been recently transferred by helicopter from a smaller hospital. She had started bleeding from a duodenal ulcer and was in serious condition. The attending surgeon and medical consultant both agreed to try aggressive medical management because of the risks of surgery. But she continued to bleed despite treatment and was taken to surgery that evening. It was a Saturday night, and I was getting a report from the surgical resident who had been on call the previous shift. "We had quite a time with your patient last night," he said. "She survived the operation, but I think she'll probably die sometime today." "Not on my shift," I blurted out without thinking. As I went through my surgical rotation in medical school, and now in residency, this situation had come up many times. There was always one patient on a service who was close
GW25-e2369 Lipoic acid inhibits oxidative stress-induced premature senescence via upregulation of Sirt1 in human umbilical vascular endothelial cells
Authors: Wu Lin, Yong Liu, Bin Zhou, Dinghui Liu, Baoshun Hao, Ming Wang, Xiaoxian Qian
Journal: Journal of the American College of Cardiology (2014)
Full symptomatic recovery does not ensure full recovery of muscle-tendon function in patients with Achilles tendinopathy
Authors: Karin Grävare Silbernagel, Roland Thomeé, Bengt I Eriksson, Jon Karlsson
Journal: British Journal of Sports Medicine (2007)

Objective: To assess the relationship between muscle-tendon function and symptoms in patients with Achilles tendinopathy using a validated test battery. Design: A prospective non-randomised trial. Setting: Orthopaedic Department, Sahlgrenska University Hospital, Sweden. Patients: 37 patients with a clinical diagnosis of Achilles tendinopathy in the midportion of the tendon, with symptoms for &gt;2 months, were evaluated at the initiation of the study and after 1 year. Intervention: The patients were treated using a rehabilitation programme, under the supervision of a physical therapist, for 6 months. Main outcome measurements: The patients were evaluated using the Swedish version of the Victorian Institute of Sports Assessment—Achilles questionnaire (VISA-A-S) for symptoms, and a test battery for evaluation of the lower leg muscle-tendon function. Results: There were significant improvements in the VISA-A-S score (p&lt;0.00, n = 37) and the test battery (p&lt;0.02, n = 19) at the 1-year follow-up. The VISA-A-S questionnaire had an effect size of 2.1 and the test battery had an effect size of 0.73. A low correlation (r = 0.178, p&gt;0.05) was found between the VISA-A-S score and the test battery. A high correlation (r = 0.611, p&lt;0.05) was found between the drop counter movement jump and the VISA-A-S score. All other tests in the test battery had low correlations (r = −0.305 to 0.155, p&gt;0.05) with the VISA-A-S score. Only 25% (4/16) of the patients who had full symptomatic recovery had achieved full recovery of muscle–tendon function as measured by the test battery. Conclusion: Full symptomatic recovery in patients with Achilles tendinopathy does not ensure full recovery of muscle–tendon function. The VISA-A-S questionnaire and the test battery are sensitive to clinically relevant changes with treatment and can be recommended for use in both the clinic and research.