tbuckley/cabot-search huggingface dataset:
P741Transcriptome-based identification of new anti-anti-inflammatory and vasodilating properties of the omega-3 polyunsaturated fatty acid docosahexaenoic acid in vascular endothelial cells under proinfla
Authors: M Massaro, E Scoditti, MA Carluccio, R Martinelli, T Buonuomo, V Gatta, C Storelli, R De Caterina
Journal: Cardiovascular Research (2014)

A high intake of the omega-3 polynsaturated fatty acids (PUFA) docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) has been associated with anti-inflammatory effects and cardiovascular protection, but the underlying molecular basis remains incompletely defined. Using a DNA microarray technology we investigated the early gene expression profile of human vascular endothelium conditioned by DHA under pro-inflammatory conditions. Methods: Human umbilical vein endothelial cells (HUVEC) were treated with 50 μmol/L DHA for 48 hours and then stimulated with 5 ng/mL IL-1β for 3 hours. Total RNA was extracted, and qualitatively/quantitatively analyzed with a NanoDrop spectrophotometer and an Agilent Bioanalyzer before RNA labeling and purification. Gene expression profile was performed with an Agilent Whole Human Genome Oligo Microarray covering 41 000 unique genes and transcripts. Slides were scanned with the Agilent's scanner and images processed using Agilent Feature Extraction software. The raw data were further processed with the GeneSpring® 10 software and differentially expressed RNA identified using Benjamini and Hochberg False Discovery Rate with a P-value <0.05. Functional and network analyses were identified by the Ingenuity Pathways version 8.0 Analysis. Results: Fixing a significance threshold at 1.5 fold of change, DHA alone significantly altered the expression of 188 genes, down-regulating 92 and up-regulating 96. IL-1β significantly changed the expression of 2031 genes, down-regulating 997 and up-regulating 1034. Treatment with DHA, before IL-1 significantly affected the expression of 116 IL-1β -deregulated genes. Immunological, inflammatory and metabolic pathways were the most affected. In particular, newly identified DHA-regulated genes were involved in stemness, cellular growth and proliferation, cardiovascular system development and function, and cancer. These included cytochrome p450 4F2 (CYP4F2), transforming growth factor (TGF)-β2, caspase recruitment domain (CARD)11, and the junctional adhesion molecule (JAM)-A. Conclusions: Endothelial exposure to DHA regulates several novel genes and related pathways. Such unbiased identification improves our understanding of mechanisms by which omega-3 PUFAs may affect human diseases.
Abstract 1033: Inhibiting bladder cancer metastasis with a dietary supplement
Authors: Kelly T. Hoye, Travis Yates, David Alonzo, Vinata B. Lokeshwar
Journal: Cancer Research (2014)

Abstract INTRODUCTION AND OBJECTIVE: Hyaluronic acid (HA) and HYAL-1 hyaluronidase (HAase) family molecules are potential markers for BCa diagnosis and prognosis and promote tumor growth and metastasis. 4-Methylumbelliferone (4-MU) is an orally bioavailable dietary supplement that inhibits HA synthesis. Epithelial-to-mesenchymal transition (EMT) is the hallmark of invasion and metastasis. Since HA promotes tumor metastasis and EMT, we evaluated the expression of EMT markers in bladder tissues and antitumor effects of 4-MU in BCa models. METHODS: Quantitative PCR was used to measure mRNA expression of EMT genes (β-catenin, Twist, and Snail) in 66 bladder tissue specimens (27 normal; 39 tumor); follow-up: 26±4.3 months; median 20 months. The effect of 4-MU (40 - 120 µg/ml) on cell proliferation, apoptosis, intracellular signaling, and the expression of HA receptors and EMT genes were examined in BCa cell lines, 253J-Lung and HT1376. Effect of 4-MU on tumor growth was analyzed in subcutaneous xenografts. RESULTS: Among the EMT genes, Snail and Twist were differentially expressed in BCa tissues when compared to normal bladder (P&amp;lt;0.001). Furthermore, elevated Twist expression significantly correlated with metastasis (2-fold increase; P=0.028). β-catenin expression negatively correlated with survival (chi square=4.3, p=0.038). At IC50 for HA synthesis, 4-MU inhibited proliferation (∼ 2.5-fold) and induced apoptosis (3-fold) in BCa cells. 4-MU induced caspase-8, -9 and -3 activation and up-regulation of Fas and FADD. 4-MU caused 4-10-fold downregulation of HA-receptors (CD44, RHAMM), and EMT promoters - β-catenin, Snail and Twist, but increased E-cadherin expression by 2-fold. In xenograft studies, 4-MU significantly decreased tumor growth (&amp;gt; 3-fold) when treatment was started either on the day of tumor cell injection or after tumors became palpable. No weight loss or serum or organ toxicity was observed in treated mice. Tumors showed reduced microvessel density (∼3-fold) and HA expression but increased TURNEL positive cells. CONCLUSION: This study shows that expression of EMT determinants correlates with clinical outcome. Furthermore, the non-toxic dietary supplement 4-MU has potent antitumor activity and reverses EMT. Support: R01 CA 123063-04 (VBL); R01 CA 72821-11 (VBL). Citation Format: Kelly T. Hoye, Travis Yates, David Alonzo, Vinata B. Lokeshwar. Inhibiting bladder cancer metastasis with a dietary supplement. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1033. doi:10.1158/1538-7445.AM2014-1033
Handbook of Autopsy Practice
Authors: Syed A. Hoda
Journal: JAMA (2009)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Unusual Parathyroid Adenoma Avid for Both Tc-99m Sestamibi and I-123 Iodine
Authors: Anne-Sophie Pirson, Salima Bouazza, Bruno Krug, Simona Bostan, Marie-Cécile Nollevaux, Luc Michel, Julian Donckier, Thierry Vander Borght
Journal: Clinical Nuclear Medicine (2010)

Parathyroid scintigraphy has been recommended in hyperparathyroidism for preoperative localization of parathyroid adenoma to avoid long and invasive neck exploration. Dual isotope scintigraphy (Tc-99m sestamibi/Tc-99m pertechnetate or I-123 iodine) can differentiate accurately a parathyroid from a thyroid adenoma, only the latter takes up both tracers. We report an unusual case where both Tc-99m sestamibi and I-123 iodine accumulated in a pathologically proven parathyroid adenoma.
AVERTIN ANAESTHESIA IN CHILDBIRTH
Authors: G. M. B. Morgan
Journal: BMJ (1932)

admission.The spleeni was palpable one inch below the costal margin; haemoglobin was. 38per cent.She was be- tween seven and eight months pregnant, and obviously very ill; she seemed confused.A course of atebrin and plasmoquine wvas begun on January 17th.At the end of this treatment she was very much improved.Mental symptoms cleared up forty-eight hours after admission.On the fifth day haemo globin was* 45 per cent.The course was repeated on January 26th.In addition she received 1 per cent.arsenical solution -3 minims, rising to 5 minims, three times a day.She is still in hospital awaiting parturition.On February 9th her haemoglobin was 75 per cent.She is walking about and is apparently quite well.Blood findings shown on temperature chart. CASE VIIA male European, aged 34 years, was admitted on January 17th, 1932, with a history of numerous attacks of malaria.Present illness over a week.Complained of usual symptoms of fever, especially very severe headache.He was slightly jaundiced ; the spleen was just palpable ; and he had very septic teeth.Blood findings indicated on chart.At the date of wxriting (March lst) he is still in hospital awaiting dental attention, but is otherwise well.A course of atebrin and plasmoquine was given, and a second similar course was started on February 27th.CASE VIII A male European, aged 28 years, was admitted on February 19th, 1932, with a history of malaria for over a month and of previous attacks.Mentally somewhat dull, verging on coma.Decidedly jaundiced.Pulse bad.Spleen was palpable 2 cm.below costal margin.Complained of very severe headache.Blood examiination showed a mixed infection, with practically every blood cell containing a parasite.Many blood cells contained two parasites.Atebrin and plasmoquine were given as above.The results of the blood examination are recorded on the chart.H-Iaevnoglobin estimation was done with the Dare haemoglobinometer.A second course was started on February 27th.He is now convalescent.
Molecular Diagnosis of the Hematologic Cancers
Authors: Louis M. Staudt
Journal: New England Journal of Medicine (2003)

Hematologic cancers — that is, leukemias and lymphomas — usually result from somatic mutations in hematopoietic cells rather than heritable mutations. Many of these cancers have been classified on the basis of their morphology and the presence of tumor marker antigens. This review explains complementary DNA expression profiling, a genomic approach to the diagnosis of such tumors, with certain leukemias and lymphomas used as examples.
Echocardiography of the tricuspid valve in congenital left ventricular-riht atrial communication.
Authors: N C Nanda, R Gramiak, J A Manning
Journal: Circulation (1975)

Two patients, ages 14 and 18, with congenital left ventricular-right atrial communication through a defect in the tricuspid valve were studied by echocardiography. Proof was obtained by angiocardiography and surgery in one and ultrasonic contrast injection and angiocardiography in the other. Both presented clinically as uncomplicated ventricular septal defects. Echocardiography consistently demonstrated a high frequency, low amplitude flutter of the tricuspid valve in systole and none in diastole. Following surgical correction of the defect in one patient, there was complete disappearance of the systolic flutter. Systolic flutter has not been observed with tricuspid incompetence nor with other forms of ventricular septal defects. Fistulous communication from the aorta to the right atrium just above the tricuspid valve did not demonstrate systolic flutter in one patient studies. Tricuspid valve systolic flutter appears to be caused by the passage of the left ventricular jet blood into the right atrium through a defect in the tricuspid valve. Echocardiographic study of the tricuspid valve is of value in the recognition of the congenital left ventricular-right atrial communication.
Fluoxetin-induced pulmonary granulomatosis
Authors: E de Kerviler, J Tredaniel, G Revlon, O Groussard, G Zalcman, JM Ortoli, M Espie, A Hirsch, J Frija
Journal: European Respiratory Journal (1996)

A patient treated with fluoxetin for a manic depressive disorder developed pulmonary inflammatory nodules with noncaseating giant cell granulomas, interstitial pneumonia and non-necrotizing vasculitis, whilst remaining asymptomatic. A progressive resolution of pulmonary nodules occurred after withdrawal of the offending agent, and the chest radiograph returned to normal in 9 months. The diagnosis was assessed by an open lung biopsy.
399 Granulocyte-macrophage colony-stimulating factor (GM-CSF) is essential for enhancing airway responsiveness in mice with the liquid transformation product (LTP) of NO2
Authors: K OHTA, T MIIYASAKA, M NISHIZAWA, S KUBOTA, M KUWABARA, R ITO, A ISHII, T HORIUCHI, K MANO, T MIYAMOTO
Journal: Journal of Allergy and Clinical Immunology (1996)
Epidermal Growth Factor Receptor Blockade in Combination with Conventional Chemotherapy Inhibits Soft Tissue Sarcoma Cell Growth <i>In vitro</i> and <i>In vivo</i>
Authors: Wenhong Ren, Borys Korchin, Quan-Sheng Zhu, Caimiao Wei, Adam Dicker, John Heymach, Alexander Lazar, Raphael E. Pollock, Dina Lev
Journal: Clinical Cancer Research (2008)

Abstract Purpose: The epidermal growth factor receptor (EGFR) is highly expressed in many human soft tissue sarcomas (STS). However, EGFR blockade has not apparently been used for human STS therapy; therefore, we examined the in vitro and in vivo effects and the underlying mechanisms before considering EGFR blockade as a therapy for STS patients. Experimental Design: Human STS tissues and cell lines were used to study EGFR expression and activation. Western blot analysis was used to evaluate effects of EGFR activation on downstream signaling. Cell culture assays were used to assess the effect of EGF stimulation as well as EGFR blockade (using an EGFR tyrosine kinase inhibitor, Iressa; AstraZeneca) on STS cell growth, apoptosis, and chemosensitivity. An in vivo study (HT1080 human fibrosarcoma cell line in nude/nude mice: Iressa, doxorubicin, Iressa + doxorubicin, vehicle) was used to examine tumor growth; pEGFR, proliferating cell nuclear antigen, and terminal deoxyribonucleotide transferase–mediated nick-end labeling staining helped assess the effect of therapy in vivo on STS EGFR activation, proliferation, and apoptosis. Results: EGFR was expressed and activated in STS cell lines and tumors, probably due to ligand binding rather than EGFR mutation. Stimulation caused activation of AKT and mitogen-activated protein kinase pathways. EGFR blockade inhibited these effects and also caused increased apoptosis, a p53-independent G0-G1 cell cycle arrest, and decreased cyclin D1 expression. In vivo, Iressa + doxorubicin had markedly synergistic anti-STS effects. Conclusion: EGFR blockade combined with conventional chemotherapy results in anti-human STS activity in vitro and in vivo, suggesting the possibility that combining these synergistic treatments will improve anti-STS therapy.
Tumor promoters and cocarcinogens in tobacco carcinogenesis.
Authors: D Hoffmann, S S Hecht, E L Wynder
Journal: Environmental Health Perspectives (1983)

Cigarette smoke induces carcinoma of the larynx in Syrian golden hamsters and is active as a tumor promoter in hamsters pretreated with a low dose of a PAH, nitrosamine, or nitrosamide. These tumorigenic effects are only observed with total smoke, but not with the gas phase alone. This demonstrates that the tumorigenic agents reside primarily in the particulate phase. According to fractionation experiments, a number of four- and five-ring aromatic hydrocarbons serve as the major tumor initiators in tobacco smoke. Tumor promoters reside primarily in weakly polaric neutral subfractions and in the weakly acidic portion of the particulate matter and include certain unsaturated hydrocarbons and phenolic compounds. Cocarcinogenic activity is a characteristic feature of tobacco smoke and its particulates. Among the cocarcinogens formed during combustion are catechols and certain nontumorigenic aromatic hydrocarbons and terpenes. Nicotine may also serve as a cocarcinogen as is indicated by preliminary data. The action of tumor promoters and cocarcinogens in tobacco carcinogenesis, the precursors for tobacco smoke promoters and cocarcinogens, and methods for their reduction in smoke are discussed.
The Biomedical Aspects of Lasers
Authors: Leon Goldman, Donald J. Blaney, Alan Freemond
Journal: JAMA (1964)

THERE IS much to interest the physician in the development of that fascinating instrument of modern physics, the laser. Although the first working laser was produced as late as July, 1960,<sup>1</sup>laser development is now one of the greatest research fields of modern science. However, except for a few exceptions in the biomedical and microwelding fields, it remains a laboratory tool for scientific research. It is to medicine that most research workers in laser technology look for immediate practical developments, although up to the present there has not been much investigation of lasers in the field of medicine. The laser produces a beam of coherent electromagnetic radiation of a sharply defined wavelength in that region of the spectrum broadly described as optical. This range includes the near-violet, the visible, and the near and far regions in the infrared. The term "coherence" is to be emphasized because it is an
ECG Patterns Useful in the Diagnosis of Intermittent Heart Block
Authors: Jacob I. Haft
Journal: JAMA (1972)

Blockages in any of the three intraventricular conduction pathways (the right bundle branch, and anterior superior radiations and posterior inferior radiations of the left bundle branch) have specific effects on the QRS pattern as seen on the standard electrocardiogram. The presence of block in two of the three pathways (manifested by right bundle branch block and left axis deviation, right bundle branch block and right axis deviation, alternating complete right and left bundle branch block, or to a lesser extent left bundle branch block) has been found in many patients with documented intermittent heart block or in patients who subsequently develop permanent complete heart block. The presence of this QRS pattern in patients with histories of syncope or dizziness suggests that their symptoms may be due to intermittent heart block. Such patients should be monitored to document heart block, or should have permanent demand pacemakers implanted, or both.
Abstract P6-12-01: A Meta Analysis of Risk of Cardiovascular Events in Patients with Metastatic Breast Cancer (MBC) Treated with Anti Vascular Endothelial Growth Factor (VEGF) Therapy — Bevacizumab
Authors: S Nasim, BA Sousa, RA Dent, KI. Pritchard
Journal: Cancer Research (2010)

Abstract Background and objectives: Bevacizumab, used in combination with chemotherapy has demonstrated efficacy in randomized trials in MBC. Hypertension, congestive heart failure and cardiomyopathy have been reported in trials of bevacizumab in breast cancer. The aim of this systematic review and meta analysis is to determine the overall risk of grade 3-4 hypertension (HTN), left ventricular dysfunction (LVD) and venous (VT) and arterial thromboembolic events (ATE) related to Bevacizumab in patients with MBC. Methods: We selected randomized phase III trials which compared chemotherapy with or without bevacizumab in MBC as first or second line therapy. Data extraction was carried out from results published in the literature or from conference proceedings of the selected studies. For the analysis, we used a fixed-effects and random effects models to calculate the pooled event-based relative risk ratios (RR) with 95% confidence interval (CI). The Cochran's Q statistic and I2 statistics were first calculated to assess the heterogeneity among the proportions of the included trials. We collected all grade 3-4 events reported in these trials in relation to cardiovascular events: HTN, LVD, congestive heart failure, cardiomyopathy, VT and ATE. Results: Five trials were included in the meta-analysis: E2100, AVADO, RIBBON-1, RIBBON-2 and Miller et al study (capecitabine alone or plus bevacizumab in previously treated MBC). In total 2126 patients received chemotherapy in combination with bevacizumab and 1444 chemotherapy alone. When there was tendency for heterogeneity, random-effects models were used. Thromboembolic events were pooled together as VT and ATE as information was not clear in some of the reported studies. CHF and cardiomyopathy were considered for LVD events in the Miller study as LVD was not reported. Patients treated with bevacizumab-containing regimens had a RR for HTN of 10.32 (95% CI, 4.23- 24.79; p&amp;lt;.0001), RR for LVD 2.58 (95% CI, 1.06 - 6.32; p=0.04) and RR for VT and ATE of 1.71 (95% CI, 0.81- 3.60; p=0.16) as shown in figure 1. Conclusion: HTN is a recognized side effect of bevacizumab therapy. The risk of LVD is also significantly higher with bevacizumab therapy (RR 2.58) as shown in this pooled analysis, but the risk of thrombotic events is not increased. Figure available in online version. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr P6-12-01.
Transfemoral Pacing With Balloon-Tipped Catheters
Authors: Steven G. Meister
Journal: JAMA (1973)

A method for rapid, bedside institution of temporary pacing has been developed utilizing floating, balloon-tipped, bipolar pacing catheters inserted percutaneously via a femoral vein. Successful pacing was accomplished without fluoroscopy in 30 of 31 patients. Ease and rapidity of insertion have been remarkable. The preformed "J" configuration of the catheter plus the transfemoral route of insertion aid in the attainment of a stable pacing position at the apex of the right ventricle.
IDIOPATHIC EPILEPSY.OBSERVATIONS ON ITS TREATMENT.
Authors: DANIEL R. BROWER
Journal: JAMA (1905)

This paper is not intended to be an exhaustive treatise on the therapeutics of this disease. The ideal treatment for epilepsy is that of the Craig colony at Sonyea, and our best efforts should be given to establishing such colonies at the earliest possible moment in every state in which they do not already exist. After we thus make provision for many of these unfortunate people, however, there will still remain a large number who will require treatment at their own homes or in our offices.  In considering the therapeutics of epilepsy in general, it is well to bear in mind the fact that there are spontaneous cures, and one of these cases was brought to my attention for the first time about twenty years ago. I was called to see a man of 60 in convulsions. He had had an attack of grip, with high temperature and suppression of urine;
REMOVAL OF SLENDER FOREIGN BODIES FROM THE STOMACH AND DUODENUM WITHOUT GASTROTOMY OR DUODENOTOMY
Authors: Paul W. Greeley
Journal: JAMA (1933)

Recently two articles<sup>1</sup>have appeared inThe Journalconcerning the removal of foreign bodies from the stomach and duodenum. The methods described were very similar and, while not claimed to be original, are not often used and deserve further mention so as to emphasize the value of this type of procedure. Briefly, the treatment consists in the passage of a catheter or small stomach tube through the mouth down into the stomach. Through an abdominal incision, the stomach is exposed and the foreign body "milked" into the tube. The tube is then withdrawn, taking the foreign body with it. The abdomen is then closed without the necessity of opening any hollow viscus. This method is chiefly applicable to slender objects such as "Bobby Pins," hairpins and safety pins. An open safety pin can be closed by pressure through the stomach wall and converted into a slender body. A slight