tbuckley/cabot-search huggingface dataset:
Achondrogenesis type IB is caused by mutations in the diastrophic dysplasia sulphate transporter gene
Authors: Andrea Superti-Furga, Johanna Hästbacka, William R. Wilcox, Daniel H. Cohn, Hans J. van der Harten, Antonio Rossi, Nenad Blau, David L. Rimoin, Beat Steinmann, Eric S. Lander, Richard Gitzelmann
Journal: Nature Genetics (1996)
Proliferating or interleukin 1-activated human vascular smooth muscle cells secrete copious interleukin 6.
Authors: H Loppnow, P Libby
Journal: Journal of Clinical Investigation (1990)

The cells that make up blood vessel walls appear to participate actively in local immune and inflammatory responses, as well as in certain vascular diseases. We tested here whether smooth muscle cells (SMC) can produce the important inflammatory mediator IL6. Unstimulated SMC in vitro elaborated 5 X 10(3) pg recIL6/24h (i.e., biological activity equivalent to 5 X 10(3) pg recombinant IL6 (recIL6), as determined in B9-assay with a recIL6 standard). Several pathophysiologically relevant factors augmented IL6 release from SMC including 10 micrograms LPS/ml (10(4) pg recIL6), 10 ng tumor necrosis factor/ml (4 X 10(4) pg recIL6), and most notably 10 ng IL1/ml (greater than or equal to 3.2 X 10(5) pg recIL6). Production of IL6 activity corresponded to IL6 mRNA accumulation and de novo synthesis. SMC released newly synthesized IL6 rapidly, as little metabolically labeled material remained cell-associated. In supernatants of IL1-stimulated SMC, IL6 accounted for as much as 4% of the secreted proteins. In normal vessels SMC seldom divide, but SMC proliferation can occur in hypertension or during atherogenesis. We therefore tested the relationship between IL6 production and SMC proliferation in response to platelet-derived growth factor (PDGF) in vitro. Quiescent SMC released scant IL6 activity, whereas PDGF (1-100 ng/ml) produced concentration-dependent and coordinate enhancement of SMC proliferation and IL6 release (linear regression of growth vs. IL6 release yielded r greater than 0.9). IL6 itself neither stimulated nor inhibited SMC growth or IL6 production. Intact medial strips studied in short-term organoid culture produced large quantities of IL6, similar to the results obtained with cultured SMC. These findings illustrate a new function of vascular SMC by which these cells might participate in local immunoregulation and in the pathogenesis of various important vascular diseases as well as in inflammatory responses generally.
Digitalis after AMI.
Authors: D Shander
Journal: Circulation (1977)

Clearly eq (5) is analytically equivalent to eq (4).When im- plemented on computers of adequate precision, the relationships used by Shell and our group also lead to numerically equivalent results.Shell states that the correlation (r) between histologic and en- zymatic estimates can be improved from .2 to .5 by adjusting for control enzyme levels (regression between columns 1 and 2 in Shell's letter).Table 10, in fact, demonstrates that the correlation can be improved to .84 (r 2 = 0.71) by limiting analysis to the small infarcts and adjusting for control enzyme levels.This table was apparently overlooked by Shell.Also overlooked was the analysis of the influence of estimates from defibrillated animals on the correlation with histologic infarct size.Our approach to such problems involves assessment of correla- tion with and without that data rather than elimination from the study on the basis of pre-conceptions.Concerning the suggested methodological difference, it is impor-
Detection of Epstein-Barr virus in primary cutaneous amyloidosis
Authors: Y.T. CHANG, H.N. LIU, C.K. WONG, K.C. CHOW, K.Y. CHEN
Journal: British Journal of Dermatology (1997)

To determine the association of Epstein-Barr virus (EBV) with primary cutaneous amyloidosis (PCA), a retrospective study was conducted on skin tissue from 27 Chinese patients with lichen amyloidosus and macular amyloidosis. In situ hybridization with oligonucleotide probes was used to detect the expression of EBV-encoded RNAs (EBERs). Eleven of 27 cases (40.7%) were found to contain the EBV genome. No EBV genome was detected in the skin of the control groups, including three cases of secondary cutaneous amyloidosis, two cases of primary systemic amyloidosis, and four cases of lichen simplex chronicus. Our study showed no correlation between the presence of EBV in PCA patients and the patients'age, sex, clinical type or severity of the skin lesions. Although our results suggest that EBV may be associated with some cases of PCA, the true aetiological role of EBV in PCA remains unknown.
Changes in the expression of surface receptors on lymphocyte subsets in the elderly: Quantitative flow cytometric analysis
Authors: Lia Ginaldi, Massimo De Martinis, Annamaria D'Ostilio, Lucia Marini, Francesca Loreto, Marco Modesti, Dennis Quaglino
Journal: American Journal of Hematology (2001)

The immunophenotype of circulating lymphocytes, including the intensity expression of surface receptors, changes with ageing. Until now, no results of systematic studies on age-dependent changes with respect to the expression of the major lymphocyte surface receptors in healthy elderly subjects have been reported. In order to identify age-related changes in both representation and immunophenotype of lymphocyte populations, we investigated, by means of triple-color whole-blood immunostaining and quantitative flow cytometry, the percent values and the absolute numbers, as well as the levels of surface antigen expression or antigen molecules per cell (ABC values x 10(3)), of different peripheral blood lymphocyte subsets from 23 healthy elderly subjects and 13 young donors. Naive (CD45RA+CD3+) T cells, total B cells, and CD5+ B lymphocytes are decreased (22%, 6%, 0.8% vs. 30%, 12%, 1.4%, respectively), whereas activated (HLA-DR+CD3+) and memory (CD45RO+CD3+) T cells, CD3+CD7- T lymphocytes, and lymphocytes expressing the NK marker CD56 are expanded in the elderly (2%, 53%, 13%, 6% vs. 0.8%, 45%, 8%, 8%, respectively). Moreover, T lymphocytes from elderly individuals express lower CD3 (61 +/- 10) compared to young (69 +/- 10). Considering the different T-cell populations, CD3 antigen is respectively decreased on CD45RO+ T cells (55 +/- 14 vs. 66 +/- 14) and up-regulated on CD56+ T lymphocytes (62 +/- 21 vs. 45 +/- 20). Increased CD8 expression characterizes CD3+CD7- lymphocytes (70 +/- 34 vs. 44 +/- 17) while HLA-DR on activated T cells is lower in old (39 +/- 7) than young (46 +/- 9) donors. CD7 is down-regulated both in T (22 +/- 3 vs. 28 +/- 3) and NK (48 +/- 18 vs. 71 +/- 18) cells, whereas CD2 expression, unchanged on NK cells, is up-regulated on T lymphocytes (54 +/- 10 vs. 41 +/- 8). Age-related changes in B-cell antigen expressions were also found: CD20 is increased (124 +/- 23 vs. 105 +/- 16) whereas, despite the unchanged CD5 expression of T cells, CD5 intensity on the B-cell subset co-expressing this antigen is higher in old (49 +/- 37) than in young (22 +/- 4) people. The observed changes in the expression of functionally important cellular receptors can contribute to the remodeling of immune function characteristic of the elderly. Moreover, since quantitative flow cytometry is becoming widely employed in clinical practice, our results also contribute to the assessment of specific age-dependent antigen expression changes to be considered for diagnostic approaches in the elderly.
Abstract 19: Identification of a novel mode of kinase inhibitor resistance: An F604S exchange in FIP1L1-PDGFRA modulates FIP1L1-PDGFRA protein stability in a SHP-2 and SRC-dependent manner
Authors: Gorantla P. Sivahari, Nikolas Von Bubnoff, Ana Lena Illert, Nicolas Schneider, Christian Peschel, Justus Duyster
Journal: Cancer Research (2010)

Abstract FIP1L1-PDGFR alpha is a constitutively activated protein kinase which was reported in chronic eosinophilic leukemia (CEL) and in cases of hypereosinophilic syndrome and mastocytosis with eosinophilia. Imatinib is clinically active against FIP1L1-PDGFRA positive disease. However, clinical resistance to imatinib has been observed in FIP1L1-PDGFRA positive leukemia and was shown to occur due to a secondary mutation (T674I) in the PDGFR alpha kinase domain. Using a screening strategy to identify imatinib resistant mutations, we generated numerous imatinib resistant cell clones. Analysis of the PDGFRA kinase domain in these cell clones revealed a broad spectrum of resistance mutations including the clinically reported exchange T674I. Interestingly, one of the abundant mutations was a Phe to Ser exchange at position 604 (F604S), which occurred alone or in combination with other exchanges. Surprisingly, FIP1L1-PDGFRA/F604S in contrast to D842H and F604+D842H did not increase the biochemical or cellular IC50 value to imatinib when compared to wild-type (wt). However, F604S and F604S+D842H transformed Ba/F3, NIH3T3 and mouse bone marrow more efficiently compared to wt and D842H, respectively. Immunoprecipitation and immunoblotting indicated increased amounts of FIP1L1-PDGFRA protein in F604S versus wt cells. Pulse chase analysis revealed that FIP1L1-PDGFRA/F604S is strongly stabilised compared to wt. SRC coimmunoprecipitated with FIP1L1-PDGFRA in wt, but not F604S cells. Co-expression of SRC in 293T cells augmented degradation of wt, but not F604S FIP1L1-PDGFRA, indicating that SRC is a negative regulator of FIP1L1-PDGFRA protein stability. Importantly both, the SRC inhibitor PD166326 and SRC siRNA mimicked the F604S phenotype and resulted in stabilization of the wt protein. Importantly, phosphatase inhibitor treatment of FIP1L1-PDGFRA/F604S led to destabilization and SRC recruitment indicating that phosphatases might be responsible for the enhanced stability of FIP1L1-PDGFRA/F604S. In fact, coimmunuprecipitaion experiments identified the phosphatase SHP2 as a specific binding partner of F604S mutated FP. Together, these results suggest that stabilization of FIP1L1-PDGFRA/F604S is due to dephosphorylation by SHP-2 leading to lesser activation of the SRC and Cbl mediated ubiquitination machinery. In summary, imatinib resistance screening identified a novel class of resistance mutations in FIP1L1-PDGFRA, that do not act by impeding drug binding to the target, but increased target stability and abundance by interfering with SRC- mediated degradation. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 19.
Multifunctional pH-Disintegrable micellar nanoparticles of asymmetrically functionalized β-cyclodextrin-Based star copolymer covalently conjugated with doxorubicin and DOTA-Gd moieties
Authors: Tao Liu, Xiaojie Li, Yinfeng Qian, Xianglong Hu, Shiyong Liu
Journal: Biomaterials (2011)
EXERCISE Tc99m-TEB0R0XIME CARDIAC SPECT: RESULTS OF A CANADIAN MULTICENTRE TRIAL
Authors: R J Burns, A Fung, S Iles, R Davies, L Daigneault, L Lalonde, F Hong Tai
Journal: Clinical Nuclear Medicine (1991)

S of the Society of Nuclear Medicine in Canada and Prairie Provinces Chapter, SNM Conjoint Meeting: PDF Only
Hepatitis B Immunity in Hospital Staff Targeted for Vaccination
Authors: George F. Grady
Journal: JAMA (1982)

To estimate how many hospital workers might be exempted from immunization with newly available but expensive hepatitis B vaccine, baseline serum samples were analyzed to determine prevalence and titer of hepatitis B (HB) surface antibody (anti-HBs) and prevalence of core antibody (anti-HBc). Among 1,370 persons tested, anti-HBs was present in 279 (20.4%), including 192 (14.0%) with titers of 10 ratio units or greater. In a subset of 746 subjects who denied recent exposures, and who were tested for anti-HBc and anti-HBs, at least one of the two antibodies was found in 172 (23.1%). There were 47 (6.3%) who had anti-HBs alone and 27 (3.6%) with anti-HBc alone. Prevalence was related more closely to clinical service (eg, renal dialysis or surgery) than to title (eg, physician or technician).
Functional characteristics of intraepithelial lymphocytes from mouse small intestine. III. Inability of intraepithelial lymphocytes to induce a systemic graft-versus-host reaction is because of failure to migrate in vivo.
Authors: M E Baca, A M Mowat, S MacKenzie, D M Parrott
Journal: Gut (1987)

In this study we have investigated whether addition of bone marrow accessory cells or concurrent administration of recombinant IL-2 would allow intraepithelial lymphocytes (IEL) to induce a systemic, lethal GvHR in irradiated hosts. In addition we have studied the ability of IEL to migrate into lymphoid tissues after intravenous injection and compared this with their locomotor capacity in vitro.
Holidays, Birthdays, and Postponement of Cancer Death
Authors: Donn C. Young
Journal: JAMA (2004)

ContextArticles in the medical literature and lay press have supported a belief that individuals, including those dying of cancer, can temporarily postpone their death to survive a major holiday or other significant event, but results and effects have been variable.ObjectiveTo determine whether, for the patient dying of cancer, a "death takes a holiday" effect showing a reduction in deaths in the week before a significant event was associated with Christmas, the US holiday of Thanksgiving, or the date of the individual's birthday.Design, Setting, and SubjectsAnalysis of death certificate data for all 1 269 474 persons dying in Ohio from 1989-2000, including 309 221 persons dying with cancer noted as the leading cause of death.Main Outcome MeasureWe measured the total number of cancer deaths in the 2 weeks centered on the event of interest and the proportion of these deaths that occurred in the week before the event to determine whether this proportion was significantly different from 0.5 by using an exact binomial test.ResultsThe proportion of persons dying of cancer in the week before Christmas, Thanksgiving, and the individual's birthday was not significantly different from the proportion dying in the week after the event (P = .52, .26, and .06, respectively). However, among black individuals there was an increase in cancer deaths in the week before Thanksgiving (P = .01), whereas women showed an increase in cancer deaths in the week before their birthday (P = .05). There was no statistically significant excess of deaths in the postevent week in any subgroup.ConclusionWe found no evidence, in contrast to previous studies, that cancer patients are able to postpone their deaths to survive significant religious, social, or personal events.
1148-224 Abnormalities in magnetocardiographic atrial signals in patients with focally triggered lone atrial fibrillation
Authors: Rajia Koskinen, Heikki Väänänen, Mika Lehto, Juha Montonen, Lasse Lehtonen, Liisa-Maria Voipio-Pulkki, Lauri Toivonen
Journal: Journal of the American College of Cardiology (2004)
Maternal Low-Level Lead Exposure and Fetal Growth
Authors: Motao Zhu, Edward F. Fitzgerald, Kitty H. Gelberg, Shao Lin, Charlotte M. Druschel
Journal: Environmental Health Perspectives (2010)

BackgroundLimited epidemiologic studies have examined the association between maternal low-level lead exposure [blood lead (PbB) < 10 μg/dL] and fetal growth.ObjectiveWe examined whether maternal low-level lead exposure is associated with decreased fetal growth.MethodsWe linked New York State Heavy Metals Registry records of women who had PbB measurements with birth certificates to identify 43,288 mother–infant pairs in upstate New York in a retrospective cohort study from 2003 through 2005. We used multiple linear regression with fractional polynomials and logistic regression to relate birth weight, preterm delivery, and small for gestational age to PbB levels, adjusting for potential confounders. We used a closed-test procedure to identify the best fractional polynomials for PbB among 44 combinations.ResultsWe found a statistically significant association between PbB (square root transformed) and birth weight. Relative to 0 μg/dL, PbBs of 5 and 10 μg/dL were associated with an average of 61-g and 87-g decrease in birth weight, respectively. The adjusted odds ratio for PbBs between 3.1 and 9.9 μg/dL (highest quartile) was 1.04 [95% confidence interval (CI), 0.89–1.22] for preterm delivery and 1.07 (95% CI, 0.93–1.23) for small for gestational age, relative to PbBs ≤ 1 μg/dL (lowest quartile). No clear dose–response trends were evident when all of the quartiles were assessed.ConclusionsLow-level PbB was associated with a small risk of decreased birth weight with a supralinear dose–response relationship, but was not related to preterm birth or small for gestational age. The results have important implications regarding maternal PbB.
Role of splenectomy in the management of hemophilic patients with human immunodeficiency virus‐associated immunopathic thrombocytopenic purpura
Authors: Cindy A. Leissinger, W. Abe Andes
Journal: American Journal of Hematology (1992)

Abstract Immunopathic thrombocytopenic purpura (ITP) can be a life‐threatening complication of human immunodeficiency virus (HIV) infection in patients with hemophilia and can seriously compromise quality of life if not managed effectively. We report here complete response to splenectomy in four severe hemophiliacs with HIV‐associated ITP. All patients were symptomatic, had platelet counts less than 25,000/mm 3 , and had failed at least one non‐surgical therapy prior to splenectomy. All patients tolerated surgery well and obtained an immediate and durable complete response. In addition to our experience, a review of the literature shows that splenectomy is well tolerated and provides the most effective long‐term solution for hemophiliacs with HIV‐ITP. © 1992 Wiley‐Liss, Inc.
Low-Dose Intravenous Infusion of Insulin in Diabetic Coma
Authors: Samuel Vaisrub
Journal: JAMA (1974)

Despite available methods of monitoring the clinical and metabolic state of a diabetic in ketoacidotic coma, uncertainties remain about the optimum dosage of required insulin and the frequency of its administration. Even though the half-life of subcutaneously injected insulin is known to be four to five hours, its absorption rate in ketoacidotic coma is often unpredictable. Equally unforeseeable are the vagaries of absorption by way of the intramuscular route. Added to these imponderables is apprehension that insulin may become less effective as coma deepens. Recent reports suggest that much uncertainty can be eliminated by continuous low-dose intravenous infusion of insulin. First reported by Sönksen<sup>1</sup>in 1972, this approach has been tried recently in several medical centers. Three reports, published in the June 29 issue of the<i>British Medical Journal</i>, attest to its success. From England comes the report of a collaborative project of four hospitals.<sup>2</sup>Thirty-eight comatose diabetics
Aerosol-induced Immunoglobulin (Ig)-E Unresponsiveness to Ovalbumin Does Not Require CD8+ or T Cell Receptor (TCR)-γ/δ+ T Cells or Interferon (IFN)-γ in a Murine Model of Allergen Sensitization
Authors: Brian W.P. Seymour, Laurel J. Gershwin, Robert L. Coffman
Journal: The Journal of Experimental Medicine (1998)

Mice exposed for 20 min daily to aerosolized ovalbumin (OVA) for 10 d at concentrations from 1 to 0.01% OVA made greatly reduced immunoglobulin (Ig)-E responses to subsequent immunogenic OVA challenges, given either intraperitoneally or by aerosol. This IgE-specific unresponsiveness lasted for at least four months. However, these aerosol-treated mice were primed for larger OVA-specific IgG1 and IgG2a responses. The specific reduction in IgE responses was not due to preferential induction of a T helper (Th)-1 response as aerosol OVA– primed mice made greatly reduced Th2 and no detectable Th1 response after rechallenge in vitro. Consistent with this, the increase in circulating eosinophils observed in control Th2-primed mice was absent in aerosol OVA–treated animals. Interferon (IFN)-γ was not required for this unresponsiveness, as IFN-γ knockout mice and anti–IFN-γ antibody-treated wild-type mice had greatly reduced levels of IgE similar to wild-type controls. CD8+ T cells played a relatively small role as IgE responses were reduced to about the same extent in β2 microglobulin-deficient, or in anti-CD8–treated wild-type mice as in normal mice after aerosol OVA treatment. Similarly, T cell receptor (TCR)-γ/δ T cells were not required for maximal inhibition of the IgE response. These results demonstrate that exposure to inhaled protein antigens can induce a state of unresponsiveness of CD4+ T cells that results in a prolonged loss of IgE and eosinophil responses to subsequent challenges. This T cell unresponsiveness was shown not to require CD8+ or TCR-γ/δ+ T cells or IFN-γ.
Changes in blood pressure during isometric contractions to fatigue in the cat after brain stem lesions: effects of clonidine
Authors: C. A Williams, J. R Roberts, D. B Freels
Journal: Cardiovascular Research (1990)

Journal Article Changes in blood pressure during isometric contractions to fatigue in the cat after brain stem lesions: effects of clonidine Get access Carole A Williams, Carole A Williams Department of Physiology, College of Medicine, East Tennessee State University, Johnson City, TN 37614-0002, USA Correspondence to: Dr Williams Search for other works by this author on: Oxford Academic PubMed Google Scholar Jon R Roberts, Jon R Roberts Department of Physiology, College of Medicine, East Tennessee State University, Johnson City, TN 37614-0002, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Douglas B Freels Douglas B Freels Department of Physiology, College of Medicine, East Tennessee State University, Johnson City, TN 37614-0002, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 24, Issue 10, October 1990, Pages 821–833, https://doi.org/10.1093/cvr/24.10.821 Published: 01 October 1990 Article history Received: 23 November 1989 Accepted: 04 May 1990 Published: 01 October 1990
<i>Cabeza (Back of Head)</i>
Authors: Carrie A. Butt
Journal: JAMA (2014)

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