tbuckley/cabot-search huggingface dataset:
Enhancement by norepinephrine of automaticity in sheep cardiac Purkinje fibers exposed to hypoxic glucose-free Tyrode's solution: a role for alpha-adrenoceptors?
Authors: A Mugelli, S Amerini, G Piazzesi, E Cerbai, A Giotti
Journal: Circulation (1986)

A period of drive in the presence of norepinephrine (NE) may be followed by the induction or acceleration of spontaneous activity. Experiments were carried out in sheep cardiac Purkinje fibers to determine whether the effects of NE on automaticity were modified during superfusion with hypoxic glucose-free Tyrode's solution and to assess the possible contribution of alpha-adrenergic influences on automaticity under these conditions. The following results were obtained: Low concentrations of NE (10(-7) and 3 X 10(-7)M) were able to induce automaticity after a period of drive in normal oxygenated (97% O2, 3% CO2) Tyrode's solution. Superfusion with hypoxic (97% N2, 3% CO2) glucose-free Tyrode's solution enhanced NE-induced automaticity. Practolol, in concentrations able to block the effects of NE in normal oxygenated solution, did not counteract the effects of NE in hypoxic glucose-free solution. Yohimbine, but not prazosin, antagonized the effects of NE in hypoxic glucose-free solution. At the same concentration, yohimbine did not affect transmembrane potentials or automaticity induced by isoproterenol. It is concluded that alpha-adrenergic responsiveness appears to be enhanced during superfusion in vitro with hypoxic glucose-free solution, and that alpha-adrenoceptors belonging to the alpha 2-subtype in sheep cardiac Purkinje fibers might influence abnormal automaticity, possibly through an effect on oscillatory potentials.
Hidradenitis suppurativa. Complications resulting in death
Authors: S. L. Moschella
Journal: JAMA (1966)

HIDRADENITIS suppurativa is a chronic, recurrent, indolent, suppurative infection of the apocrine glands of the axillae, areolae of the breast, inguinal regions, external genitalia, and perianal areas. The early lesion resembles a furuncle without a necrotic core. Later in the disease, subcutaneous abscesses, fistulas, intercommunicating sinus tracts, and irregular hypertrophic scarring are seen. The loose areolar tissue present in the involved sites facilitates the burrowing characteristic of the disease.  This disease occurs equally in both sexes at or after puberty. Menstrual and premenstrual exacerbations have been reported.1Axillary involvement is more common in females; the perianal involvement is more frequent in males. One in six patients with axillary involvement has perianal lesions; however, only one in 13 cases of this disease has only perianal involvements.2  Brunsting3emphasized the association of hidradenitis suppurativa with acne conglobata and dissecting cellulitis of the neck and scalp. He pointed out that
Homeodomain Protein Otp and Activity-Dependent Splicing Modulate Neuronal Adaptation to Stress
Authors: Liat Amir-Zilberstein, Janna Blechman, Yehezkel Sztainberg, William H.J. Norton, Adriana Reuveny, Nataliya Borodovsky, Maayan Tahor, Joshua L. Bonkowsky, Laure Bally-Cuif, Alon Chen, Gil Levkowitz
Journal: Neuron (2012)
How Do Practicing Physicians Use New Drugs?
Authors: Harry F. Dowling
Journal: JAMA (1963)

WE LAUGH AT Molière's humor, yet we recognize the kernel of truth in his quip. Most visits to the doctor do result in the patients' swallowing pills, and what is more, the patients seem to want it that way. As Osler put it, "The desire to take medicine is one feature which distinguishes man... from his fellow creatures."<sup>1</sup>Not only are many drugs used, but every drug of significance is prescribed or administered by a physician. Thus, the fruits of the labor of thousands of scientists in laboratories, the mountains of data amassed by these and other investigators, the millions of words spawned by the advertising copywriters, all must be beamed to a single spot—to the brain of the individual physician, who must decide whether or not to give a particular drug to a particular patient. Experts may advise, salesmen may cajole, and patients may plead. But the physician must decide.
Pulmonary Diseases and Disorders
Authors: Herbert P. Wiedemann
Journal: JAMA (1989)

In this second edition,<i>Pulmonary Diseases and Disorders</i>assumes almost monumental proportions, having expanded to 2563 pages in three volumes. The initial flicker of foreboding experienced by this reviewer upon receiving the textbook was quickly extinguished by its attractive cover design, clean page format, and lavish use of color illustrations. I subsequently found that the value of<i>Pulmonary Diseases and Disorders</i>is not limited to its considerable visual appeal. The textbook is comprehensive, authoritative, and current in its coverage of pulmonary medicine. Furthermore, sections of this textbook are entertaining, engaging, and worthy of casual, nondirected reading in a manner rarely found in leading academic textbooks. Particularly noteworthy in this regard are the early sections that trace the historical development of our understanding of pulmonary physiology and remind us of the seminal contributions of the early pioneers, including Harvey, Laennec, Haldane, Bernard, Henderson, Barcroft, Fick, and Krogh. Pulmonary Diseases and Disorders
Induction of HSP70 in cultured rat neonatal cardiomyocytes by hypoxia and metabolic stress.
Authors: K Iwaki, S H Chi, W H Dillmann, R Mestril
Journal: Circulation (1993)

BACKGROUND A cultured neonatal rat cardiomyocyte model is used to investigate the expression of the inducible heat shock protein 70 (HSP70i) during hypoxia/reoxygenation and metabolic stress. METHODS AND RESULTS The major HSP70i is increased in its expression at the mRNA and protein level in myocytes exposed to hypoxia/reoxygenation and metabolic stress by the addition of 2-deoxyglucose and sodium cyanide, which are inhibitors known to block ATP production. Surprisingly, the appearance of HSP70 mRNA precedes the intracellular ATP depletion caused by hypoxia, which is contrary to what we observe when the cardiomyocytes are subjected to metabolic stress. CONCLUSIONS It has been postulated recently that the decrease in intracellular ATP content in cells under stress may be the trigger that leads to the induction of HSP70i by reducing the pool of free HSP70, thus activating the stress response. Our results indicate that although this may be the case during metabolic stress, another route of activation must be used during the early stages of hypoxia in cardiomyocytes. The induction of HSP70i also appears to precede the onset of cellular damage as measured by the release of cytoplasmic enzymes and preincorporated arachidonic acid. This indicates that cardiomyocytes are able to respond to hypoxia/reoxygenation and metabolic stress with increased HSP70i production and points to a potential protective role of heat shock proteins during ischemia/reperfusion injury.
Validation of ERCC1-XPF Immunodetection – Response
Authors: Laura J. Niedernhofer, Nikhil R. Bhagwat, Richard D. Wood
Journal: Cancer Research (2010)

Immunodetection and quantification of ERCC1-XPF in tumors may well develop into a clinically relevant technique, but it requires investigation with an antibody specific for the nuclease. Our article emphasizes that the reliability of immunohistochemistry depends upon rigorous selection of a specific antibody (1). As we pointed out, the antibody 8F1 does recognize ERCC1 without complications in HeLa cells, and this is apparently also the case for some other cancer cell lines as noted by Olaussen and Soria (in their Fig. 1A and B). Nevertheless, the existence of a strong cross-reacting protein in four human fibroblast cell lines raises great concern about the specificity of 8F1 and the validity of using it to draw conclusions about ERCC1 expression in tissue samples. The antibody FL297 is highly specific for ERCC1, if imperfect for immunohistochemistry. FL297 seems to stain nuclei in the control sample and the cytoplasm in the ERCC1 knockdown sample of Olaussen and Soria (their Fig. 1C), the same staining pattern we reported in Fig. 5 of our publication using genetic mutants (1). In contrast, 8F1 gives a varying degree of nuclear staining in all samples. We interpret the differential staining of normal and ERCC1-depleted cells with FL297 as an opportunity to test whether immunodetection of ERCC1 is clinically relevant. Readers are encouraged to examine the evidence presented in our article for the cross-reactivity of 8F1. It will indeed be of interest to identify the cross-reacting protein detected by 8F1. In the meantime, we and others are developing new antibodies and subjecting them to thorough quality control.

Disclosure of Potential Conflicts of Interest R.D. Wood: coinventor with Cancer Research UK of antibody 8F1; advisory board, DNAR. The other authors disclosed no potential conflicts of interest.
SERIAL CHANGES OF NEOINTIMAL COVERAGE CONDITIONS OF SIROLIMUS-ELUTING STENTS IMPLANTATION: FOLLOW-UP OPTICAL COHERENCE TOMOGRAPHY STUDY
Authors: Daisuke Nakamura, Masami Nishino, Yasuharu Lee, Takahiro Yoshimura, Masayuki Taniike, Nobuhiko Makino, Hiroyasu Kato, Yasuyuki Egami, Ryu Shutta, Jun Tanouchi, Yoshio Yamada
Journal: Journal of the American College of Cardiology (2011)
Psychiatry in the Undergraduate Medical Curriculum
Authors: MORTON F. REISER
Journal: American Journal of Psychiatry (1973)

Psychiatry in its teaching activities has the obligation to develop effective ways of bridging vast theoretical, empirical, and philosophical gaps that exist between nonpsychiatric physicians and biological scientists (without backgrounds in behavioral science) on the one hand, and behavioral and social scientists and other nonmedical professionals engaged in health care (without backgrounds in biology and medicine) on the other hand.
A memorable patient: Sometimes a detailed examination is warranted
Authors: A. Hamon
Journal: BMJ (1997)

A 17 year old woman presented to the accident and emergency department with a letter from her GP requesting a fast track physiotherapy referral. She had a four month history of a painful stiff neck, unresponsive to analgesia, which the GP thought might be relieved by intermittent traction or ultrasound treatment.

My initial thought as the casualty senior house officer was that a trial of diazepam, as a muscle relaxant, might break the pain-spasm-pain cycle, but my second …
859 Unexpected identification of the B lymphocyte transcription factor PAX5 in mouse immortalized biliary epithelial cells (IBECS) stimulated with lipopolysaccharide or proinflammatory cytokines
Authors: H WANG, M BRAUN, J VIERLING
Journal: Hepatology (2003)

Biliary epithelial cells (BECs) of chronic non-suppurative destructive cholangitis (CNSDC) in primary biliary cirrhosis (PBC) reportedly express p21WAF1/Cip1 and p16INK4a, which may induce cell cycle arrest and are related to progressive loss of BECs of PBC. Given that the ATM pathway plays a role in the induction of p21WAF1/Cip1, we examined its possible involvement in bile duct damage of PBC. The expression of phosphorylated-ATM (p-ATM) reflecting the activation of ATM, p21WAF1/Cip1 and 8-hydroxy-deoxyguanosine (8-OHdG), an oxidative stress marker, was examined immunohistochemically in the liver tissues of 20 cases of stage 1/2 PBC, 9 extrahepatic biliary obstruction (EBO), 35 chronic viral hepatitis (CVH), 17 nonalcoholic steatohepatitis (NASH), and 18 histologically normal liver. p21WAF1/Cip1, p-ATM and 8-OHdG were frequently and extensively co-expressed in the nuclei of CNSDC in PBC, and their expressions were correlated. In contrast, the expression of these three molecules was absent or faint in small bile ducts in normal livers, CVH, and EBO, and these molecules were clearly expressed in the nuclei of hepatocytes of NASH, in which oxidative stress is involved in hepatocellular damage. In conclusion, oxidative stress-induced p21WAF1/Cip1 expression in BECs in PBC is closely associated with activation of the ATM pathway and the resultant reduced regeneration or cell cycle arrest of BECs may be related to the progressive loss of small bile ducts of PBC.
NATURAL HISTORY OF TIC DOULOUREUX, WITH REMARKS ON TREATMENT.
Authors: CHARLES L. DANA
Journal: JAMA (1900)

If we hope to judge wisely as to how much good certain forms of treatment do for tic douloureux, we must know its natural history, which is not yet very fully understood. For though the disease is common, its origin is often unexplainable and there is none in which so much desperate effort is made to break up the course of the attacks and interfere with the natural symptoms. I have thought, therefore, that I could serve the purposes of this discussion best by devoting a good part of my time to this side of the subject. It is one of the advantages of somewhat mature years that we are able to take a wider view of the course and of the history of the diseases as shown in our patients, and I have been able to make a close study of a number of my own cases from the
TCT-763 A Risk Prediction Algorithm for 1-Year Mortality after Transcatheter Aortic Valve Implantation
Authors: Moritz Seiffert, Jan-Malte Sinning, Sandra Wilde, Lenard Conradi, Mariuca Vasa-Nicotera, Alexander Ghanem, Christoph Hammerstingl, Francisco Ojeda, Dietmar Koschyk, Johannes Schirmer, Stephan Baldus, Eberhard Grube, Hermann Reichenspurner, Georg Nickenig, Stefan Blankenberg, Patrick Diemert, Nikos Werner, Hendrik Treede, Renate Schnabel
Journal: Journal of the American College of Cardiology (2013)
UK must learn from US's mistakes in tackling research misconduct
Authors: Clare Dyer
Journal: BMJ (2007)

The United Kingdom should learn from mistakes made by the United States in setting up mechanisms to tackle research malpractice, a leading US expert told delegates at the UK's first multidisciplinary conference on research fraud last week.

Nicholas Stenek, a consultant to the US Office of Research Integrity (ORI) and professor emeritus of the University of Michigan, said that the key policy question was to determine what needs to be done to ensure that improper practices don't compromise the public's investment in research.

Stressing that his comments were his own views and not those of the ORI, he said that the US systems …
Severe and Prolonged Renal Insufficiency
Authors: Bruce R. Bacon
Journal: JAMA (1978)

RECOVERY of renal function in patients with malignant hypertension complicated by severe renal insufficiency has been reported previously, but is rare.<sup>1</sup>When dialysis is instituted in such patients, it usually has to be maintained for life even though the hypertension may be controlled. Our patient had malignant hypertension and severe renal insufficiency; with control of hypertension and with regular dialysis, he has recovered sufficient renal function so that he no longer requires dialysis. <h3>Report of a Case</h3> A 44-year-old man was admitted to Cleveland Metropolitan General Hospital on Aug 13, 1975, with dizziness, headaches, confusion, blurred vision, shortness of breath, and marked oliguria of recent onset. There was no past history of renal disease. He had been told that he had hypertension in the past, but he was never treated. On admission, blood pressure was 270/170 mm Hg. Examination of the fundi showed bilateral papilledema, arteriolar narrowing, hemorrhages, and
A Note on Methylene and other Anaesthetics
Authors: S. Wells
Journal: BMJ (1888)

with little expense or danger, and some important discoveries might have been made" (The Doql, by Williim Youatt, 1851).Surely the public, at any r.te, whlo place themselves in our hands, under these the wxorst of circumstatnces, woutld unlhesitatingly choose to be helped to die patinlessly.And in following such a course no practitioner would he violitting, by neglect, tlhe great ethical principle of his profession, that he shotull en(leaLoLir as far as lies in his power to preserve life as well as alleviate pain.
LIGNEOUS PHLEGMON OF THE ABDOMINAL WALL
Authors: W. W. GRANT
Journal: JAMA (1913)

It is probable that this disease has been observed under some name or form more frequently than has been reported. Prior to Reclus' publication and report, in 1893, of five cases which he denominated ligneous phlegmon of the neck, nothing seems to have been known of this severe, protracted and still little understood disease. Since that time professional interest has been enlisted and broadened, and in the last five or six years, especially, substantial additions have been made to the subject in case reports and contributions. Still the literature, though it is interesting and instructive, is not replete in quantity, nor entirely satisfactory as to etiology, bacteriologic findings and treatment.  These reports and investigations lead the way to a final explanation of the disease, which at the present time is the most interesting and unusual, if not curious and erratic, manifestation of infection, or inflammation, with which we have to
Spontaneous regression of chronic lymphocytic leukemia: clinical and biologic features of 9 cases
Authors: Ilaria Del Giudice, Sabina Chiaretti, Simona Tavolaro, Maria Stefania De Propris, Roberta Maggio, Francesca Mancini, Nadia Peragine, Simona Santangelo, Marilisa Marinelli, Francesca Romana Mauro, Anna Guarini, Robin Foà
Journal: Blood (2009)
Follicles, fungi and scalp conditions
Authors: A.G. Messenger
Journal: British Journal of Dermatology (2011)

Towards the end of the 19th century seborrhoeic dermatitis and dandruff (SD ⁄D) were ascribed to a small micro-organism, originally thought to be a bacterium but almost certainly a small lipophilic yeast of the genus Malassezia, previously known as Pityrosporum.In the 1960s and 1970s this idea was replaced by the concept of SD ⁄D as a hyperproliferative disorder in which microbial colonization is a secondary event.However, in the late 1970s and early 1980s the observation that SD ⁄D responds to treatment with azole antifungal agents led to a revival of interest in a causative role for Malassezia.Although difficult to prove due to its commensal status on human skin, the evidence that Malassezia plays a key role in the pathogenesis of SD ⁄D is convincing and widely accepted.In recent years there have been major advances in the biology of Malassezia, for example through the identification of multiple subspecies and the sequencing of the M. globosa and M. restricta genomes.This work has led to significant advances in our understanding of the pathogenesis of SD ⁄D and its treatment, which are highlighted in the reviews published here.Following an update on human cutaneous fungal infections focusing on Malassezia, new data are presented on the role of the hair follicle infundibulum, both as a key anatomical region in the pathogenesis of SD ⁄D and as a target for drug delivery.Novel information on the mechanism of action of zinc pyrithione, the most widely used antidandruff agent, and its effective delivery to the infundibulum is also presented.
Further Perspectives on Concierge Medicine
Authors: James Bowers
Journal: Annals of Internal Medicine (2010)

Letters17 August 2010Further Perspectives on Concierge MedicineJames Bowers, MDJames Bowers, MDFrom Primary Care Partners Northwest, Seattle, WA 98133.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-153-4-201008170-00018 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:In his Perspective essay, Stillman (1) misses an important point. I sense that today's primary care physicians, especially those in direct employment with a hospital or health group, have a “shift mentality”—that is, they work their 8-to-5 shift and go home. Concierge doctors fill the need for customers seeking time and access. Also, instead of only coming in to see a doctor when a patient has a “complaint,” a concierge practice fosters wellness through prevention. I admit I do a better job when I get to spend time with each patient, rather than getting rushed from patient ...Reference1. Stillman M. Concierge medicine: a “regular” physician's perspective. Ann Intern Med. 2010;152:391-2. [PMID: 20231570] LinkGoogle Scholar Author, Article, and Disclosure InformationAffiliations: From Primary Care Partners Northwest, Seattle, WA 98133.Disclosures: None disclosed. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoConcierge Medicine: A “Regular” Physician's Perspective Michael Stillman Further Perspectives on Concierge Medicine Harry M. Walen Further Perspectives on Concierge Medicine Marcy Zwelling-Aamot Further Perspectives on Concierge Medicine Robert A. Swerlick Further Perspectives on Concierge Medicine Robert P. Fields Further Perspectives on Concierge Medicine Christine Harter Metrics 17 August 2010Volume 153, Issue 4Page: 275KeywordsConflicts of interest ePublished: 17 August 2010 Issue Published: 17 August 2010 CopyrightCopyright © 2010 by American College of Physicians. All Rights Reserved.PDF DownloadLoading ...
Ivabradine Treatment Blunts Dobutamine-Induced Increase in Heart Rate in Patients with Acute Decompensated Heart Failure: A Comparative Study with Beta-Blocker Therapy
Authors: Yüksel Çavuşoğlu, Kadir Uğur Mert, Aydın Nadir, Fezan Mutlu, Erkan Gencer, Bektas Morrad, Taner Ulus
Journal: Journal of the American College of Cardiology (2013)
Immunization with Irradiated Tumour Cells and Specific Lymphocyte Cytotoxicity in Malignant Melanoma
Authors: G. A. Currie, F. Lejeune, G. H. Fairley
Journal: BMJ (1971)

A microculture technique has been adapted to assay the cytotoxic properties of the lymphocytes from patients with malignant melanoma when cultured with their autologous tumour cells. In a series of patients with established melanoma specific autologous cytotoxicity was extremely uncommon, being detectable in only 3 out of the 22 cases studied. This cytotoxicity did not correlate with clinical staging of the disease but may well have been related to tumour volume. By autoimmunization of patients with an irradiated suspension of their own tumour cells the appearance of cytotoxic lymphocytes could be provoked in 5 out of the 12 patients studied. This cytotoxicity was detectable at the end of the first week after the autograft and disappeared by the third week. Cytotoxic lymphocytes did not correlate in any obvious way with the appearance of specific antitumour antibodies detected by immunofluorescence. So far there has been no evidence of a serum factor capable of blocking the lymphocyte cytotoxicity in these patients. The presence and possible significance of cytotoxic lymphocytes in patients with malignant disease is discussed.
1087-106 Early serum levels of tissue inhibitor of metalloproteinases-1 but not matrix metalloproteinase-1 are associated with cardiovascular death after complicated myocardial infarction
Authors: Cord A Manhenke, Stein Ørn, Faiez Zannad, Torbjørn Aarsaland, Kenneth Dickstein
Journal: Journal of the American College of Cardiology (2004)
Switching prescription drugs to over the counter: Consumers may benefit financially in examples from the US
Authors: Patrick W Sullivan
Journal: BMJ (2005)

EDITOR—Cohen et al say that switching drugs to over the counter increases costs for most insured patients in the United States because of resultant changes in drug benefits.1 Most health plans restrict drug coverage and increase copayments when one member of the class is made available over the counter. However, evidence from recent switches from prescription to over the counter shows that …
Persistent and therapeutic concentrations of human factor IX in mice after hepatic gene transfer of recombinant AAV vectors
Authors: Richard O. Snyder, Carol H. Miao, Gijsbert A. Patijn, S. Kaye Spratt, Olivier Danos, Dea Nagy, Allen M. Gown, Brian Winther, Leonard Meuse, Lawrence K. Cohen, Arthur R Thompson, Mark A. Kay
Journal: Nature Genetics (1997)