tbuckley/cabot-search huggingface dataset:
Effects of the C57BL/6 strain background on tauopathy progression in the rTg4510 mouse model
Authors: Rachel M Bailey, John Howard, Joshua Knight, Naruhiko Sahara, Dennis W Dickson, Jada Lewis
Journal: Molecular Neurodegeneration (2014)

Abstract Background Cross-breeding of transgenic mice is commonly used to assess gene-gene interactions, particularly in the context of disease. Strain background changes can influence the phenotype of mouse models and can confound crossbreeding studies. We sought to determine if changing the strain background of a commonly used mouse model of tauopathy (rTg4510) would significantly impact the originally reported phenotype. On the original F1 FVB/N x 129S6 background, rTg4510 mice present with progressive cognitive decline, increased insoluble tau, robust tau pathology and age-dependent neurodegeneration. One of the most common strains in mouse modeling is C57BL/6. We and others have previously reported that this strain background alters the phenotypes of various models, including the JNPL3 model of tauopathy. To determine if the phenotype of rTg4510 mice was similarly affected by the introduction of the C57BL/6 background, we compared rTg4510 mice on the original F1 FVB/N x 129S6 background to rTg4510 mice on an F1 FVB/N x C57BL/6NTac (B6/NTac) background, herein termed rTg4510 B6 . Results Despite a small, but significant increase in soluble human tau levels, young rTg4510 B6 mice had equivalent levels of tau phosphorylation, aggregation and cognitive impairments as age-matched rTg4510 mice. At 6.5 months of age, rTg4510 B6 mice displayed hyperphosphorylated insoluble tau and robust cortical tau neuropathology that was equivalent to age-matched rTg4510 mice; however, 10.5-month-old rTg4510 B6 mice had greater amounts of phospho-tau in the cortex and hippocampus when compared to age-matched rTg4510 mice. Non-transgenic (NT) littermates of rTg4510 B6 (NT B6 ) mice also had greater amounts of cortical and hippocampal phospho-tau at 10.5 months of age when compared to NT littermates of rTg4510 mice. Additionally, older rTg4510 B6 mice had gross forebrain neurodegeneration that was equivalent to age-matched rTg4510 mice. Conclusions Overall, our data shows that introduction of the C57BL/6 strain into the rTg4510 mouse background modestly alters the tau pathology that was originally reported in rTg4510 on the F1 FVB/129 background. In contrast, behavioral and neurodegenerative outcomes were not altered. These studies support the use of the rTg4510 mouse model on a partial C57BL/6 strain background without losing fidelity of the phenotype and suggest that the C57BL/6 background does not inherently protect against tauopathy.
Biliary Leak With Normal Gallbladder Appearance on Cholescintigraphy
Authors: Steve B. Losik, Yana Studentsova, Mahmoud Dakhel, Donald Margouleff
Journal: Clinical Nuclear Medicine (2003)

Laparascopic cholecystectomy is the current standard of care for acute cholecystitis. Biliary leak resulting from common or hepatic bile duct injuries is a well-know complication. The authors report an atypical appearance of a biliary leak into the gallbladder fossa, simulating the normal anatomy and physiology of the gallbladder, which in the absence of clinical history was initially interpreted as normal by a nuclear medicine physician. This case emphasizes the importance of obtaining accurate clinical history prior to interpreting scintigraphic studies
Abstract 2358: Molecular chaperones as a common set of proteins that regulate the invasion phenotype of head and neck cancer
Authors: Ching-Chi Chiu, Ann-Joy Cheng
Journal: Cancer Research (2011)

Abstract Purpose: The goal of this study was to establish a common set of molecules that regulate cell invasion in head and neck cancer (HNC). Experimental design: Five invasive sublines derived from HNC cell lines were established using the Matrigel selection method. Proteomic technology, MetaCore™ algorithm, and RT-PCR methods were used to search for molecules that contribute to the invasion phenotype. Cellular functional analyses and clinical association studies were applied to examine the significance of the molecules. Results: Fifty-two proteins were identified in more than two of the four independent proteomic experiments, including 10 (19%) molecular chaperones. Seven chaperones were confirmed to be differentially expressed in five sublines: Hsp90α, Hsp90β, Hsp90-B1/Gp96, Hsp70-A5/Grp78, HYOU1 up-regulation, while Hsp60 and GANAB down-regulation. Four molecules with the highest differential expressions (&amp;gt; 4-fold) were further investigated. In all cell lines, knockdown of Hsp60 or GANAB and silencing of Gp96 or Grp78 considerably enhanced or reduced cell migration and invasion, respectively. Clinical association studies consistently revealed that low levels of Hsp60 or GANAB and high levels of Gp96 or Grp78 are significantly associated with advanced cancer (P&amp;lt;0.001 to P = 0.047, respectively, for the four molecules) and poor survival (P&amp;lt;0.001 to P=0.025, respectively, for the four molecules). Conclusion: In conclusion, our study defined molecular chaperones as a common set of proteins that regulate the invasion phenotype of head and neck cancer. Loss of the tumor suppression function of Hsp60 or GANAB and acquisition of the oncogenic function of Gp96 or Grp78 contribute to aggressive cancers. These molecules may serve as prognostic markers and targets for cancer drug development. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2358. doi:10.1158/1538-7445.AM2011-2358
Combined effect of the 5-HT2 receptor antagonist DV-7028 and aspirin or heparin on coronary cyclic flow reductions in dogs
Authors: T. Tanaka, Y. Morishima, K. Watanabe, T. Shibutani, M. Yasuoka, T. Shibano
Journal: Cardiovascular Research (1993)

Journal Article Combined effect of the 5-HT2 receptor antagonist DV-7028 and aspirin or heparin on coronary cyclic flow reductions in dogs Get access Tsuyoshi Tanaka, Tsuyoshi Tanaka Exploratory Research Laboratories II, Daiichi Pharmaceutical Co Ltd, 1-16-13, Kitakasai, Edogawa-ku, Tokyo 134, Japan: T Tanaka, Y Morishima, K Watanabe, T Shibutani, M Yasuoka, T Shibano. Correspondence to Dr Shibano. Search for other works by this author on: Oxford Academic PubMed Google Scholar Yoshiyuki Morishima, Yoshiyuki Morishima Exploratory Research Laboratories II, Daiichi Pharmaceutical Co Ltd, 1-16-13, Kitakasai, Edogawa-ku, Tokyo 134, Japan: T Tanaka, Y Morishima, K Watanabe, T Shibutani, M Yasuoka, T Shibano. Search for other works by this author on: Oxford Academic PubMed Google Scholar Kazuo Watanabe, Kazuo Watanabe Exploratory Research Laboratories II, Daiichi Pharmaceutical Co Ltd, 1-16-13, Kitakasai, Edogawa-ku, Tokyo 134, Japan: T Tanaka, Y Morishima, K Watanabe, T Shibutani, M Yasuoka, T Shibano. Search for other works by this author on: Oxford Academic PubMed Google Scholar Tomoko Shibutani, Tomoko Shibutani Exploratory Research Laboratories II, Daiichi Pharmaceutical Co Ltd, 1-16-13, Kitakasai, Edogawa-ku, Tokyo 134, Japan: T Tanaka, Y Morishima, K Watanabe, T Shibutani, M Yasuoka, T Shibano. Search for other works by this author on: Oxford Academic PubMed Google Scholar Megumi Yasuoka, Megumi Yasuoka Exploratory Research Laboratories II, Daiichi Pharmaceutical Co Ltd, 1-16-13, Kitakasai, Edogawa-ku, Tokyo 134, Japan: T Tanaka, Y Morishima, K Watanabe, T Shibutani, M Yasuoka, T Shibano. Search for other works by this author on: Oxford Academic PubMed Google Scholar Toshiro Shibano Toshiro Shibano Exploratory Research Laboratories II, Daiichi Pharmaceutical Co Ltd, 1-16-13, Kitakasai, Edogawa-ku, Tokyo 134, Japan: T Tanaka, Y Morishima, K Watanabe, T Shibutani, M Yasuoka, T Shibano. Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 27, Issue 7, July 1993, Pages 1374–1379, https://doi.org/10.1093/cvr/27.7.1374 Published: 01 July 1993 Article history Received: 10 September 1992 Accepted: 02 February 1993 Published: 01 July 1993
HMOs: America today, Britain tomorrow? Nuts, bolts, and the customers.
Authors: T Richards
Journal: BMJ (1986)

One doctor I met described health maintenance organizations (HMOs) as more of a basket of fruit than a bunch of grapes but it is possible to get a taste of the whole by looking at one in detail.In this article the Harvard Community Health Plan in Boston acts as the model for the day to day running ofan HMO and the advantages and disadvantages of these organisations from a patient's point of view.
CCG funding could be based on population age, not deprivation, says Lansley
Authors: H. Jaques
Journal: BMJ (2012)

The funding allocated to England’s new clinical commissioning groups (CCGs) could be based on the age of their patient population rather than levels of deprivation, the health secretary, Andrew Lansley, has suggested.

Speaking at a conference for CCG leaders in London last month, Lansley said that age is the principal determinant of healthcare need and could drive the allocation of funding to CCGs. The funding will be determined by the NHS Commissioning Board and the Advisory Committee on Resource Allocation, an independent expert body, which will look at factors in a CCG’s population data that are likely to give rise to demand for NHS services. “What is likely to make the biggest difference is elderly populations who are not in substantial deprivation,” …
Quantitative Determination of Regional Left Ventricular Wall Dynamics by Roentgen Videometry
Authors: JEAN G. DUMESNIL, ERIK L. RITMAN, ROBERT L. FRYE, GERALD T. GAU, BARRY D. RUTHERFORD, GEORGE D. DAVIS
Journal: Circulation (1974)

The use of roentgen videometry as a means of studying regional left ventricular wall dynamics and performance was evaluated in 32 patients undergoing coronary arteriography. Nine patients had normal coronary arteriograms and hemodynamic findings (group 1), 8 patients had generalized decrease in left ventricular contraction and abnormal hemodynamic findings (group 2), and 15 patients with coronary artery disease had regional wall dynamics abnormalities (group 3). Sixty-per-second determinations of wall thickness were performed in selected sites of the left ventricle. Measurements performed included end-diastolic wall thickness (EDTw), mean and peak rates of systolic wall thickening (m and p dTw/dt), and fractional systolic increase in wall thickness (ΔTw/EDTw). In patients with uniformly performing ventricles (groups 1 and 2), these parameters correlated well with other parameters of ventricular function. Best distinction between the "normal" group (group 1) and the "abnormal" group (group 2) was achieved when the rates of thickening (m and p dTw/dt) were utilized ( P &lt; 0.001). In patients of group 3, three types of abnormal regional wall dynamics could be determined and quantified objectively: hypokinesia (decreased p dTw/dt), akinesia (p dTw/dt = 0), and dyskinesia (p dTw/dt &lt; 0). The severity of the abnormality of the wall dynamics correlated well with the presence or absence of a previous infarction on the electrocardiogram, and the anatomic location was strongly correlated with the distribution and severity of coronary artery lesions within a given ventricle.
New Method for Calculating Right Ventricular Ejection Fraction Using Gated Myocardial Perfusion Studies
Authors: ISIS GAYED, FERNANDO BOCCALANDRO, BING FANG, DONALD PODOLOFF
Journal: Clinical Nuclear Medicine (2002)

Background Quantification of right ventricular ejection fraction (RVEF) is important in patients who have right heart failure or cor pulmonale. When Tl-201 was the primary radiotracer used to evaluate myocardial perfusion, the outline of the right ventricle could vary and was not visualized in most patients. However, visualization of the right ventricle has become easier with the use of Tc-99m–labeled myocardial perfusion agents. Purpose This study describes a new method for quantifying RVEF using gated stress myocardial perfusion (GMP) slices. The results are compared with those of first-pass radionuclide ventriculography (FPRNA) in the same patients. Methods Fifty-two consecutive patients referred for routine GMP imaging were included. After administration of Tc-99m tetrofosmin, all patients underwent FPRNA using a single crystal gamma camera and a GMP study. Regions of interest (ROI) were drawn to outline the right ventricular cavity at end diastole and end systole from three pairs of GMP slices. The RVEF was calculated from the number of pixels within the ROIs. The mean RVEF obtained using FPRNA and GMP imaging was 51.8 ± 10.8% and 51.9 ± 12.3%, respectively. The two methods showed good correlation with r = 0.81. In addition, there was no significant difference in the RVEFs calculated using these methods (P = 0.85). Bland-Altman analysis also showed good agreement between the two methods (limits of agreement +14.4% to -14.0%, slope = 0.19). Intraobserver and interobserver correlation were evaluated by reanalyzing 12 patients using the new RVEF quantification method and were good at r = 0.87 and 0.82, respectively. Therefore, this is a new convenient method for evaluating RVEF as part of a routine tomographic gated myocardial perfusion study.
LYSOSOMAL α-N-ACETYLGALACTOSAMINIDASE DEFICIENCY: A NEW INHERITED METABOLIC DISEASE
Authors: O.P. Van Diggelen, D. Schindler, W.J. Kleijer, J.M.G. Huijmans, H. Galjaard, H.U. Linden, J. Peter-Katalinic, H. Egge, U. Dabrowski, M. Cantz
Journal: The Lancet (1987)
Eosinophils mediate the pathogenesis of halothane-induced liver injury in mice
Authors: William R. Proctor, Mala Chakraborty, Lynette S. Chea, Jeffrey C. Morrison, Julia D. Berkson, Kenrick Semple, Mohammed Bourdi, Lance R. Pohl
Journal: Hepatology (2012)

Drug-induced liver injury (DILI) is a major health issue, as it remains difficult to predict which new drugs will cause injury and who will be susceptible to this disease. This is due in part to the lack of animal models and knowledge of susceptibility factors that predispose individuals to DILI. In this regard, liver eosinophilia has often been associated with DILI, although its role remains unclear. We decided to investigate this problem in a murine model of halothane-induced liver injury (HILI). When female Balb/cJ mice were administered halothane, eosinophils were detected by flow cytometry in the liver within 12 hours and increased thereafter proportionally to liver damage. Chemokines, eotaxin-1 (CCL11) and eotaxin-2 (CCL24), which are known to attract eosinophils, increased in response to halothane treatment. The severity of HILI was decreased significantly when the study was repeated in wildtype mice made deficient in eosinophils with a depleting antibody and in eosinophil lineage-ablated ΔdblGata−/− mice. Moreover, depletion of neutrophils by pretreating animals with Gr-1 antibody prior to halothane administration failed to reduce the severity of HILI at antibody concentrations that did not affect hepatic eosinophils. Immunohistochemical staining for the granule protein, major basic protein, revealed that eosinophils accumulated exclusively around areas of hepatocellular necrosis. Conclusion : Our findings indicate that eosinophils have a pathologic role in HILI in mice and suggest that they may contribute similarly in many clinical cases of DILI.
Combined Live Rubella-Mumps Virus Vaccine Findings in Clinical-Laboratory Studies
Authors: Robert E. Weibel
Journal: JAMA (1971)

Four lots of combined bivalent HPV-77 duck rubella-Jeryl Lynn mumps vaccine (Biavax) were administered to a total of 415 children 7 months to 7 years of age who were initially without antibody against either virus. Overall, 94% of the children developed rubella hemagglutination-inhibiting antibodies and 96% developed mumps neutralizing antibodies. The geometric mean antibody titers were 1:35.9 and 1:6.8, respectively. The antibody responses approximated those obtained in previous studies when the individual vaccines were given alone. Clinical reactions including joint involvement and fever were notably absent among the children during the 28-day follow-up. The combined rubella-mumps vaccine provides a simple means for immunizing against both these diseases without impairment of immune response and without significant clinical reaction.
Presence of beta human papillomaviruses in nonmelanoma skin cancer from organ transplant recipients and immunocompetent patients in the West of Scotland
Authors: L.J. Mackintosh, M.N.C. de Koning, W.G.V. Quint, J. ter Schegget, I.M. Morgan, R.M. Herd, M.S. Campo
Journal: British Journal of Dermatology (2009)

Journal Article Presence of beta human papillomaviruses in nonmelanoma skin cancer from organ transplant recipients and immunocompetent patients in the West of Scotland Get access L.J. Mackintosh, L.J. Mackintosh Division of Pathological Sciences, Institute of Comparative Medicine, University of Glasgow, Glasgow, U.K.Department of Dermatology, Western Infirmary, Glasgow, U.K. Correspondence: Lorna Mackintosh. E‐mail: L.Mackintosh@clinmed.gla.ac.uk Search for other works by this author on: Oxford Academic Google Scholar M.N.C. De Koning, M.N.C. De Koning DDL Diagnostic Laboratory, Voorburg, the Netherlands Search for other works by this author on: Oxford Academic Google Scholar W.G.V. Quint, W.G.V. Quint DDL Diagnostic Laboratory, Voorburg, the Netherlands Search for other works by this author on: Oxford Academic Google Scholar J. Ter Schegget, J. Ter Schegget DDL Diagnostic Laboratory, Voorburg, the Netherlands Search for other works by this author on: Oxford Academic Google Scholar I.M. Morgan, I.M. Morgan Division of Pathological Sciences, Institute of Comparative Medicine, University of Glasgow, Glasgow, U.K. Search for other works by this author on: Oxford Academic Google Scholar R.M. Herd, R.M. Herd Department of Dermatology, Western Infirmary, Glasgow, U.K. Search for other works by this author on: Oxford Academic Google Scholar M.S. Campo M.S. Campo Division of Pathological Sciences, Institute of Comparative Medicine, University of Glasgow, Glasgow, U.K. Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 161, Issue 1, 1 July 2009, Pages 56–62, https://doi.org/10.1111/j.1365-2133.2009.09146.x Published: 01 July 2009
Textbook of Disorders and Injuries of the Musculoskeletal System: An Introduction to Orthopaedics, Fractures and Joint Injuries, Rheumatology, Metabolic Bone Disease and Rehabilitation
Authors: B. F. MORREY
Journal: JAMA (1984)

This second edition appears 13 years after the initial effort to organize and teach injury and disorders of the musculoskeletal system on a level intelligible to the orthopedic student. While the first edition was well received and represents a notable contribution to this field, the second edition has followed in this tradition and has been substantially updated according to current orthopedic thought. The strengths of this text parallel those of its predecessor. Probably one of the most impressive features is the masterful organization of the broad, complex, and difficult scope of orthopedics. The synthesis and organization of this body of knowledge are superb.  The first section deals with the scientific approach to understanding orthopedic pathology. Not only is the structure and development of bone and cartilage concisely related, but troublesome clinical terms, such as varus and valgus, anteversion and retroversion, are clearly defined in this section; this difficult terminology might
Lichen planus and primary biliary cirrhosis
Authors: R. A. C. GRAHAM-BROWN, I. SARKANY, S. SHERLOCK
Journal: British Journal of Dermatology (1982)

The association of lichen planus and primary biliary cirrhosis in five patients is reported. The coexistence of the two diseases is probably more than coincidental and may be due to the fact that both conditions are based on an alteration of mechanisms. There is great similarity between primary biliary cirrhosis and chronic graft-versus-host disease and a common pathogenesis for the two entities has been postulated. Since lichen planus is one of the most common manifestations of graft-versus-host disease, its association with primary biliary cirrhosis is of significance, although not unexpected.
Minor units take a growing share of emergency department attendances
Authors: G. Iacobucci
Journal: BMJ (2013)

Attendances at emergency departments in England are rising faster than population growth, new figures have shown.

Statistics for 2012-13 from the Health and Social Care Information Centre (HSCIC) show an 11% growth in emergency department attendances since four years ago (2008-09), compared with a population growth of 3.2% during the same period.

There were 21.7 million emergency department attendances in 2012-13, compared with an estimated 340 million consultations in general practice.

But although the HSCIC identified “striking consistencies” in patterns of emergency department activity over time, the latest figures show a higher proportion of cases being dealt with by minor units than was the case four years ago. Minor units dealt with almost 32% of attendees in 2012-13, up from 28% in …
Arterial Blood Gases and Pulmonary Function Testing in Acute Bronchial Asthma
Authors: Richard M. Nowak, Michael C. Tomlanovich, Diane D. Sarkar, Paul A. Kvale, John A. Anderson
Journal: JAMA (1983)

Pretreatment and posttreatment arterial blood gas and pulmonary function testing measurements were prospectively compared as to their ability to assess asthma severity accurately and, thus, predict the outcome in 102 episodes of acute bronchial asthma initially seen in the emergency department. The Pao2, Paco2, or pH was unable to separate these patients requiring admission from those that could be confidently discharged, while the 1-s forced expiratory volume (FEV1) and peak expiratory flow rate (PEFR) did so both before and after treatment. Furthermore, virtually all patients with hypercarbia (Paco2 greater than 42 mm Hg) and/or severe hypoxemia (Pao2 less than 60 mm Hg) had a PEFR below 200 L/min, or an FEV1 below 1.0 L. Thus, selective use of arterial blood gas analysis should substantially decrease both diagnostic cost and patient discomfort without jeopardizing health care.
Absence of antiphospholipid antibodies in Behcet's disease.
Authors: M Karmochkine, M C Boffa, B Wechsler, J C Piette, P Godeau
Journal: Annals of the Rheumatic Diseases (1993)

Letters to the editor/Matters arising that it does cause significant morbidity.Furthermore, although in this series cases were drawn from multiple disciplines, six of the 12 patients presented to the rheumatology unit, highlighting the need for rheumatologists to be aware of the condition.On plain x ray films, sacral fractures are notoriously difficult to detect, particularly in association with osteopenia.The diagnosis hinges on clinical suspicion and the correct interpretation of technetium-99m bone scanning.The diagnosis can be confirmed by computed tomography, but in the typical setting this is not mandatory.