tbuckley/cabot-search huggingface dataset:
Accurate proteome-wide protein quantification from high-resolution 15N mass spectra
Authors: Zia Khan, Sasan Amini, Joshua S Bloom, Cristian Ruse, Amy A Caudy, Leonid Kruglyak, Mona Singh, David H Perlman, Saeed Tavazoie
Journal: Genome biology (2011)

In quantitative mass spectrometry-based proteomics, the metabolic incorporation of a single source of 15N-labeled nitrogen has many advantages over using stable isotope-labeled amino acids. However, the lack of a robust computational framework for analyzing the resulting spectra has impeded wide use of this approach. We have addressed this challenge by introducing a new computational methodology for analyzing 15N spectra in which quantification is integrated with identification. Application of this method to an Escherichia coli growth transition reveals significant improvement in quantification accuracy over previous methods.
Release of somatostatin-like immunoreactivity from the perfused canine thyroid. Selective stimulatory effect of calcium ions.
Authors: P Laurberg, H Orskov
Journal: Journal of Clinical Investigation (1981)

It is well accepted that the C cells of the thyroid contain somatostatin, but the role in local endocrine function has not yet been firmly established in this organ, and it has not been proved that thyroidal somatostatin is released into the circulation. We have measured the contents of somatostatin-like immunoreactivity in the effluent of canine thyroid glands perfused without recirculation with a synthetic buffer medium. During basal conditions a definite release was consistently found in the order of 10 pg/ml corresponding to 12 pg/min. The somatostatin-like immunoreactivity was studied in dilution experiments and by gel-filtration chromatography, and found to have properties identical to those of synthetic cyclic somatostatin, which was also recovered quantitatively when added to sampling tubes. Various compounds were infused in concentrations that are highly active in pancreas perfusion experiments. 14-min infusion of arginine, 5 and 11.5 mmol/liter; isoproterenol, 10 and 23.7 nmol/liter and 68.7 mumol/liter; acetylcholine, 5 mumol/liter, carbamylcholine, 10 and 100 mumol/liter; glucagon, 1 and 30 nmol/liter; and porcine calcitonin, 1 and 100 ng/ml did not affect the basal release of somatostatin-like immunoreactivity significantly. Neither did an increase from the control level of 4 mmol/liter glucose of 10 or 20 mmol/liter, nor an increase in the control level of 4.4 mmol/liter K+ to 7.5 or 14.4 mmol/liter. Each of these compounds were tested in three or four dogs. The effect of an increase in Ca++ from the control level of 1.5 mmol/liter to 2.25, 3.0, and 4.5 mmol/liter was tested in random order in five thyroid lobes. All three doses elicited an immediate increase in effluent somatostatin-like immunoreactivity. In most experiments the response was biphasic with an early spike, followed by a stable level that was maintained during prolonged Ca++ infusion. The secretory response was not diminished through a series of repeated short pulses of calcium infusion. The response to 3.0 mmol/liter Ca++ (control period 8.4 +/- 1.5, test period 337 +/- 110 pg/ml, mean +/- SE) and 4.5 mmol/liter Ca++ (control period 9.5 +/- 1.4, test period 386 +/- 125) were significantly higher than 2.25 mmol/liter Ca++ (control period 7.2 +/- 1.0 test period 140 +/- 39), while there was no significant difference between responses to the two high doses. Infusion of salmon calcitonin, 10 ng/ml and 1 microgram/ml; or porcine calcitonin, 1 microgram/ml during calcium stimulation (2.25 mmol/liter of Ca++) did not induce alterations in the release of somatostatin-like immunoreactivity. The results demonstrate that thyroidal somatostatin is mobilizable, and it appears to be selectively sensitive to calcium stimulation, indicating a possible role in calcitonin release control.
Inhibition of Dll4-mediated signaling induces proliferation of immature vessels and results in poor tissue perfusion
Authors: Jeffrey S. Scehnet, Weidong Jiang, S. Ram Kumar, Valery Krasnoperov, Alexandre Trindade, Rui Benedito, Dusan Djokovic, Cristina Borges, Eric J. Ley, Antonio Duarte, Parkash S. Gill
Journal: Blood (2007)
US charges 107 medical professionals with $452m in fraudulent bills to Medicare
Authors: J. H. Tanne
Journal: BMJ (2012)

<b>Objective:</b> This study was to evaluate the effect of the five-element music therapy based on the midnight-noon ebb-flow theory to improve the sleep quality among COPD patients. <b>Methods:</b> A total of 60 patients were randomly divided into the experimental group and the control group, with 30 patients in each group by the random number table method from March 2016 to November. The control group received the routine nursing care, while the experimental group received midnight-noon ebb-flow of five-element music therapy based on routine nursing care. Play the music for experimental group:1, It is advisable to volume in 50-60 dB.2, Play the Yang of Zhi music between 11:00-13:00(the heart time), and play the Yang of Shang music between 3:00-5:00(the lung time) for 30min each time. 3, We made 8 weeks in the intervention.Follow-up made after 4 weeks of the intervention . The Pittsburgh sleep quality index scale (PSQI) and Flinders fatigue scale(FFS) were used to evaluate sleep quality and daytime fatigue before intervention, and 4,8 and 12 weeks after intervention. Adopting SPSS19.0 for data analysis. <b>Results:</b> The score of FFS, PSQI in the E group were lower than those in the C group after intervention (p&lt; 0.05). Further comparisons were made in the E group after the intervention in terms of PSQI and FFS, which showed the latter score was lower than the former score with significant difference(p&lt;0.05). <b>Conclusion:</b> The midnight-noon ebb-flow of five-element music therapy can effectively improve the sleep quality and daytime fatigue among patients with COPD by anxiety.
Strong selection of virus-specific cytotoxic CD4+ T-cell clones during primary human cytomegalovirus infection
Authors: Ester M. M. van Leeuwen, Ester B. M. Remmerswaal, Mirjam H. M. Heemskerk, Ineke J. M. ten Berge, Rene A. W. van Lier
Journal: Blood (2006)
Great Clinical Neurologist
Authors: H. Miller
Journal: BMJ (1970)

The year was 1959 and the patient a nubile young woman who had suddenly developed paroxysms of severe nausea and vertigo on holiday in the South of France.A physician in Nice had diagnosed a virus infection.The patient drove her sports car home to New- castle, stopping to vomit every couple of hundred miles.The symptoms cleared up, and all seemed well-until they recurred, as they did several times during the ensuing
A New Endoscopic Method for Treatment of Malignant Oesophagobronchial Fistulas
Authors: C. A. Bates, D. Beckly, J. Rahamim
Journal: Endoscopy (1991)

A short malignant oesophagobronchial fistula which could not be sealed using adhesive agents was successfully treated using a new endoscopic technique. The procedure provided good palliation and the results withstood the test of time in the patient. The method, which is described in detail, provides a useful modification to existing methods.
The Challenge of Child Abuse
Authors: Leon Tec
Journal: JAMA (1978)

Child abuse, a tragic phenomenon of life, is the subject of this book from England. However, in the United States, more than a million children a year are victims of abuse, and 2,000 children die annually in America from mistreatment. Physically abused children sent home without intervention are at a 25% to 50% risk of permanent injury or death, as reported by Kempe in 1972. Emotional abuse, almost always present with physical abuse, is even more prevalent and often has more profound effects on the child's development than physical abuse. In the volume edited by Alfred White Franklin, more than 20 British authors contribute to a comprehensive discussion of the subject. The book contains specific examples that touch on relations between deviant behavior and battering and nonaccidental injury. Abused children in England are at a high risk for damage to the CNS and maldevelopment of the personality. Severe brain damage,
Opiate receptor inhibition improves the blunted baroreflex function in conscious dogs with right-sided congestive heart failure.
Authors: S Sakamoto, C S Liang
Journal: Circulation (1989)

The endogenous opiate system is activated in congestive heart failure. because endogenous opioids are known to depress the baroreflex function, we conducted studies to determine whether the increased endogenous opioids play a role in causing the reduced baroreflex function that occurs in heart failure. Right-sided congestive heart failure was produced in 16 dogs by tricuspid avulsion and progressive pulmonary artery constriction. Seven sham-operated dogs were included for comparison. Baroreflex function was measured in the conscious dogs after pretreatment with either normal saline or an opiate-receptor antagonist by bolus administration of phenylephrine. The slope of the regression line relating systolic blood pressure to cardiac cycle (R-R) interval was taken as an index of baroreflex sensitivity. Plasma beta-endorphin was elevated in the dogs with heart failure (15.3 +/- 2.5 pmol/l) compared with the sham-operated dogs (4.2 +/- 0.4 pmol/l, p less than 0.001). The dogs with heart failure also exhibited a reduced baroreflex sensitivity (3.84 +/- 0.19 msec/mm Hg) after saline pretreatment when compared with the sham-operated dogs (10.86 +/- 1.20 msec/mm Hg, p less than 0.001). Administration of naloxone hydrochloride increased the baroreflex sensitivity of dogs with heart failure to 5.16 +/- 0.26 msec/mm Hg (p less than 0.01) but produced no significant effects in sham-operated dogs (11.36 +/- 1.42 msec/mm Hg). To further study the site of action for the effect of naloxone, we measured baroreflex sensitivity in the dogs with heart failure after pretreatment with naloxonazine, a selective mu-receptor antagonist, with ICI 154,129, a selective delta-receptor antagonist, or with naloxone methobromide, a quaternary analogue of naloxone that does not penetrate the blood-brain barrier.(ABSTRACT TRUNCATED AT 250 WORDS)
Relationship between viral antibodies and bronchial hyperresponsiveness in 495 unselected children and adolescents
Authors: V. Backer, C. S. Ulrik, N. Bach‐Mortensen, G. Glikmann, C.‐H. Mordhorst
Journal: Allergy (1993)

The purpose of this study was to investigate whether recent and previous subclinical viral respiratory infection can explain the presence of increased bronchial responsiveness to histamine. We studied a randomly selected population of 495 children and adolescents, aged 7–16 years, from Copenhagen. If the subjects had had symptoms of respiratory infection recently, the examination was postponed for at least 6 weeks. Bronchial hyperresponsiveness (BHR) to inhaled histamine was found in 79 (16%) of the subjects, of whom 28 had asthma. Forty‐eight subjects (10%) had increased levels of serum IgM antibodies against either parainfluenza, influenza, adenovirus, or respiratory syncytial virus (RSV), reflecting a recently acquired infection. No association between BHR and antibodies against respiratory viruses was found, as 7 (8.9%) of the 79 subjects with BHR and 41 (9.9%) of the 416 subjects without BHR had viral antibodies. Furthermore, no association between degree of bronchial responsiveness and viral antibodies was found. Moreover, 251 individuals (51%) had signs of earlier RSV infection, i.e. IgG antibodies against RSV. No relationship was found between age of the subjects and the presence of antibodies against either respiratory viruses in general or IgG‐RSV. No relationship was found between the presence of antibodies against RSV and BHR; furthermore, evidence of earlier RSV infection was unrelated to the level of lung function and degree of bronchial responsiveness. We conclude that increased bronchial responsiveness in asymptomatic, unselected schoolchildren and adolescents is not likely to be caused by recent or previous viral respiratory infections.
BK virus nephropathy after allogeneic stem cell transplantation: A case report and literature review
Authors: Lazaros J. Lekakis, Valentina Macrinici, Ioannis G. Baraboutis, Bonnie Mitchell, Dianna S. Howard
Journal: American Journal of Hematology (2009)

Polyomaviruses are increasingly recognized as important human pathogens. Among those, BK virus has been identified as the main cause of polyomavirus-associated nephropathy (PVAN), a major cause of renal allograft failure. PVAN has also been well described in the setting of non-renal solid organ transplantation. The reports of PVAN after hematopoietic stem cell transplantation (HCT) are surprisingly very few. Here, we describe a patient with treatment-related myelodysplastic syndrome who received an unrelated donor HCT after ablative conditioning and in vivo T cell depletion with alemtuzumab. He developed a biopsy-proven BK nephropathy, which contributed to his renal failure. Leflunomide as well as cidofovir were given at different times, both in combination with intravenous immunoglobulin. Both treatments were effective in reducing the BK viral load, the cystitis symptoms and both stabilized but did not really improved the renal function. The patient was still dialysis-dependent when he died from Pseudomonas sepsis 13 months after HCT. A critical review of the literature and the treatment modalities for post-HCT PVAN are provided.
Acute Myeloid Leukemia in First Remission: To Choose Transplantation or Not?
Authors: Richard M. Stone
Journal: Journal of Clinical Oncology (2013)

A 42-year-old woman presented with bruising and fatigue. Her WBC count was 10,370/μL, with a differential showing 5% polys, 5% monos, 10% lymphocytes, and 80% myeloid-appearing blasts, some of which contained Auer rods (Fig 1). Bone marrow examination revealed 90% infiltration with myeloid-appearing blasts, and flow cytometry analysis confirmed the diagnosis of acute myeloid leukemia (AML) with expression of CD33, CD13, and CD117. Cytogenetics revealed a normal female karyotype; molecular testing for NPM1, FLT3-ITD, and CEBPαmutations revealed wild-type status for each gene. The patient received induction therapy with daunorubicin 90 mg/m 2 per day for 3 days and continuous-infusion cytarabine 100 mg/m 2 per day for 7 days. After an induction course complicated by Gram-negative bacterial sepsis, her counts recovered by day 32, and bone marrow examination 6 weeks after diagnosis showed a complete remission. One week later she feels well and has normal physical and laboratory examinations. She is an only child (but has a common HLA type) and presents for discussion of postremission therapy options.
Multiple superficial basal cell carcinomas (basalomatosis) following cobalt irradiation
Authors: A. WOLLRNBERG, R.U. PETER, B. PRZYBILLA
Journal: British Journal of Dermatology (1995)

Basalomalosis is an uncommon skin condition characterized by the occurrence of multiple basal cell carcinomas. Many cases reported in the literature have been attribuled to arsenic treatment in psoriasis patients. We report a patient with basalomatosis caused by cobalt‐60 (60Co) irradiation. A 55‐year‐old farmer developed 43 basal cell carcinomas 20 years after treatment of an immuno‐blastoma with 60Co irradiation. All the tumours were located within the radiation fields. Other possible causes of basalomatosis, such as arsenic intoxication and basal cell naevus syndrome, were excluded. The patient's multiple superficial basal cell carcinomas probably represent a late adverse effect of the 60 irradiation.
We Are One
Authors: JAMES W. HAVILAND
Journal: Annals of Internal Medicine (1971)

Medicine is troubled by many divisive forces, external as well as internal. Apparently irreconcilable differences can be adjudicated when men of good will, earnest intentions, and integrity try. Medicine's success in harnessing scientific knowledge has also produced scientific and social problems, the solutions to which have been prematurely promised by others, thus raising undue expectations. Medicine has singleness of purpose and dedication to maintain the highest quality in education, research, service, tradition, and ethics. To achieve these goals will take all our efforts, and the American College of Physicians has the potential to aid in this achievement. The College is well suited to assume this leadership role and responsibility and to participate in this challenging struggle.
Massive Pulmonary Embolism
Authors: Nils Kucher, Elisa Rossi, Marisa De Rosa, Samuel Z. Goldhaber
Journal: Circulation (2006)

Background— Acute massive pulmonary embolism (PE) carries an exceptionally high mortality rate. We explored how often adjunctive therapies, particularly thrombolysis and inferior vena caval (IVC) filter placement, were performed and how these therapies affected the clinical outcome of patients with massive PE. Methods and Results— Among 2392 patients with acute PE and known systolic arterial blood pressure at presentation, from the International Cooperative Pulmonary Embolism Registry (ICOPER), 108 (4.5%) had massive PE, defined as a systolic arterial pressure &lt;90 mm Hg, and 2284 (95.5%) had non–massive PE with a systolic arterial pressure ≥90 mm Hg. PE was first diagnosed at autopsy in 16 patients (15%) with massive PE and in 29 patients (1%) with non–massive PE ( P &lt;0.001). The 90-day mortality rates were 52.4% (95% CI, 43.3% to 62.1%) and 14.7% (95% CI, 13.3% to 16.2%), respectively. In-hospital bleeding complications occurred in 17.6% versus 9.7% and recurrent PE within 90 days in 12.6% and 7.6%, respectively ( P &lt;0.001). In patients with massive PE, thrombolysis, surgical embolectomy, or catheter embolectomy were withheld in 73 (68%). Thrombolysis was performed in 33 patients, surgical embolectomy in 3, and catheter embolectomy in 1. Thrombolytic therapy did not reduce 90-day mortality (thrombolysis, 46.3%; 95% CI, 31.0% to 64.8%; no thrombolysis, 55.1%; 95% CI, 44.3% to 66.7%; hazard ratio, 0.79; 95% CI, 0.44 to 1.43). Recurrent PE rates at 90 days were similar in patients with and without thrombolytic therapy (12% for both; P =0.99). None of the 11 patients who received an IVC filter developed recurrent PE within 90 days, and 10 (90.9%) survived at least 90 days. IVC filters were associated with a reduction in 90-day mortality (hazard ratio, 0.12; 95% CI, 0.02 to 0.85). Conclusions— In ICOPER, two thirds of the patients with massive PE did not receive thrombolysis or embolectomy. Counterintuitively, thrombolysis did not reduce mortality or recurrent PE at 90 days. The observed reduction in mortality from IVC filters requires further investigation.
Linking self-experimentation to past and future science: Extended measures, individual subjects, and the power of graphical presentation
Authors: Sigrid S Glenn
Journal: Behavioral and Brain Sciences (2004)

The case for the value of self-experimentation in advancing science is convincing. Important features of the method include (1) repeated measures of individual behavior, over extended time, to discover cause/effect relations, and (2) vivid graphical presentations. Large-scale research on Pavlovian conditioning and weight control is needed because verification could result in easy and inexpensive mitigation of a serious public health problem.
Informed Consent and Approval by Institutional Review Boards in Published Reports on Clinical Trials
Authors: Miguel Ruiz-Canela, Miguel Ángel Martínez-González, Enrique Gómez-Gracia, Joaquín Fernández-Crehuet
Journal: New England Journal of Medicine (1999)

To the Editor: The Long-Term Intervention with Pravastatin in Ischaemic Disease (LIPID) trial (Nov.5 issue) 1 is the third study, after the Scandinavian Simvastatin Survival Study 2 and the Cholesterol and Recurrent Events study, 3 on the use of 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors for secondary prevention of coronary disease.The positive results of this trial are as marginal as those of the studies that preceded it.The authors calculated relatively high values for risk re-*Trials whose authors originally acknowledged not having obtained IRB approval or informed consent are included with trials whose authors did not originally disclose information about these issues.For comparisons of the failure to disclose information on one or both issues, the percentages of authors who failed to report on both must be added to the percentages of authors who failed to report on one.
Dextrocardia with Situs Inversus
Authors: Loh Keng Yin, Abdul Latiff Mohamed
Journal: New England Journal of Medicine (2005)

On routine physical examination, a 35-year-old healthy gardener was found to have right-sided heart sounds. Radiography of the chest showed the cardiac apex pointing to the right (Panel A). Both the aortic arch and the stomach bubble were located on the right side as well. Typical left-sided electrocardiography (Panel B) revealed an inversion of the electrical waves in leads I, II, aVR, V4, V5, and V6. Right-sided electrocardiography demonstrated a normal pattern (Panel C). Dextrocardia with situs inversus was diagnosed in this otherwise healthy man.
Clinical, Hemodynamic, and Cardiopulmonary Exercise Test Determinants of Survival in Patients Referred for Evaluation of Heart Failure
Authors: Jonathan Myers
Journal: Annals of Internal Medicine (1998)

Background: Accurate prognosis in chronic heart failure has become increasingly important in assessing the efficacy of treatment and in appropriately allocating scarce resources for transplantation. Previous studies of severe heart failure have been limited by short follow-up periods and few deaths. Objective: To establish clinical, hemodynamic, and cardiopulmonary exercise test determinants of survival in patients with heart failure. Design: Retrospective study. Setting: Hospital-based outpatient heart failure clinic. Participants: 644 patients referred for evaluation of heart failure over 10 years. Measurements: Age, cause of heart failure, body surface area, cardiac index, ejection fraction, pulmonary capillary wedge pressure, left ventricular dimensions, watts achieved during exercise, heart rate, maximum systolic blood pressure, and oxygen uptake (Vo 2) at the ventilatory threshold and at peak exercise were measured at baseline. Univariate and multivariate analyses were done for clinical, hemodynamic, and exercise test predictors of death. A Cox hazards model was developed for time of death. Results: During a mean follow-up period of 4 years, 187 patients (29%) died and 101 underwent transplantation. Actuarial 1-year and 5-year survival rates were 90.5% and 73.4%, respectively. Resting systolic blood pressure, watts achieved, peak Vo 2, Vo 2 at the ventilatory threshold, and peak heart rate were greater among survivors than among nonsurvivors. Cause of heart failure (coronary artery disease or cardiomyopathy) was a strong determinant of death (relative risk for coronary artery disease, 1.73; P < 0.01). By multivariate analysis, only peak Vo 2 was a significant predictor of death. Stratification of peak Vo 2 above and below 12, 14, and 16 mL/kg per minute demonstrated significant differences in risk for death, but each cut-point predicted risk to a similar degree. Conclusions: Peak Vo 2 outperforms clinical variables, right-heart catheterization data, exercise time, and other exercise test variables in predicting outcome in severe chronic heart failure. Direct measurement of Vo 2 should be included when clinical or surgical decisions are being made in patients referred for evaluation of heart failure or those considered for transplantation.
Effects of Modified E.C.T. on the Electrocardiogram
Authors: R. Green, A. Woods
Journal: BMJ (1955)

The results of this small series are encouraging, and seem to merit the further trial of hydrocortisone in de Quervain's disease and in tenosynovitis occurring at other sites.I wish to thank Dr. A. C. Boyle for permission to quote these cases and for his interest and many helpful criticisms