Epidemiologic Implications of the First Isolation and Cultivation of<i>Tropheryma whipplei</i>From a Saliva Sample
Authors: Bernard La Scola, Florence Fenollar, Céline Perreal, Didier Raoult
Journal: Annals of Internal Medicine (2011)

Letters15 March 2011Epidemiologic Implications of the First Isolation and Cultivation of Tropheryma whipplei From a Saliva SampleBernard La Scola, MD, PhD, Florence Fenollar, MD, PhD, Céline Perreal, and Didier Raoult, MD, PhDBernard La Scola, MD, PhDFrom Université de la Méditerranée, 13385 Marseille Cedex 05, France., Florence Fenollar, MD, PhDFrom Université de la Méditerranée, 13385 Marseille Cedex 05, France., Céline PerrealFrom Université de la Méditerranée, 13385 Marseille Cedex 05, France., and Didier Raoult, MD, PhDFrom Université de la Méditerranée, 13385 Marseille Cedex 05, France.Author, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-154-6-201103150-00018 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Background:Tropheryma whipplei causes acute diseases, such as gastroenteritis, bacteremia, and pneumonia (1), as well as chronic Whipple disease (2). It can be cultivated from stool samples, which are a potential source of infection (3). Detection of T. whipplei DNA in saliva also suggests an oral–oral method of transmission. However, cultivation of T. whipplei from saliva has been impossible because of heavy contamination by other bacteria.Methods and Findings: Examination of a T. whipplei–positive sputum sample by immunofluorescence testing revealed that it is smaller than other bacteria (Figure, left). Saliva samples were filtered, and the filtrates were inoculated on axenic medium ...References1. Fenollar F, Mediannikov O, Socolovschi C, Bassene H, Diatta G, Richet H, et al. Tropheryma whipplei bacteremia during fever in rural West Africa. Clin Infect Dis. 2010;51:515-21. [PMID: 20658941] CrossrefMedlineGoogle Scholar2. Fenollar F, Puéchal X, Raoult D. Whipple's disease. N Engl J Med. 2007;356:55-66. [PMID: 17202456] CrossrefMedlineGoogle Scholar3. Raoult D, Fenollar F, Birg ML. Culture of T. whipplei from the stool of a patient with Whipple's disease [Letter]. N Engl J Med. 2006;355:1503-5. [PMID: 17021332] CrossrefMedlineGoogle Scholar4. Renesto P, Crapoulet N, Ogata H, La Scola B, Vestris G, Claverie JM, et al. Genome-based design of a cell-free culture medium for Tropheryma whipplei. Lancet. 2003;362:447-9. [PMID: 12927433] CrossrefMedlineGoogle Scholar5. Rolain JM, Fenollar F, Raoult D. False positive PCR detection of Tropheryma whipplei in the saliva of healthy people. BMC Microbiol. 2007;7:48. [PMID: 17535423] CrossrefMedlineGoogle Scholar Author, Article, and Disclosure InformationAffiliations: From Université de la Méditerranée, 13385 Marseille Cedex 05, France.Grant Support: By the Grant of Programme Hospitalier de Recherche Clinique 2009, Assistance Publique–Hôpitaux de Marseille.Disclosures: None disclosed. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited BySeit 3 Wochen bestehende intestinale Beschwerden bei einem 67-jährigen Patienten mit chronischen ArthralgienTropheryma Whipplei Agent of Self-Limiting Infections and Whipple's DiseaseClinical Manifestations, Treatment, and Diagnosis of Tropheryma whipplei InfectionsTropheryma whipplei associated with diarrhoea in young childrenTropheryma whippleiHigh Prevalence of Tropheryma whipplei in Lao Kindergarten ChildrenTropheryma whippleiCurrent and Past Strategies for Bacterial Culture in Clinical MicrobiologyRepublished: Whipple's diseaseLooking for Tropheryma whipplei Source and Reservoir in Rural SenegalTropheryma whipplei as a commensal bacteriumFirst isolation of Tropheryma whipplei from bronchoalveolar fluid and clinical implicationsIntrafamilial Circulation of Tropheryma whipplei , FranceTropheryma whipplei: A Common Bacterium in Rural Senegal 15 March 2011Volume 154, Issue 6Page: 443-444KeywordsBacteriaChildrenDNAGastroenteritisImmunofluorescenceRabbitsResearch laboratoriesRotavirusSalivaSputum ePublished: 15 March 2011 Issue Published: 15 March 2011 CopyrightCopyright © 2011 by American College of Physicians. All Rights Reserved.PDF DownloadLoading ...