tbuckley/cabot-search huggingface dataset:
Abstract 3360: Loss of sulf-1 reprograms energy metabolism in ovarian cancer
Authors: Susmita Mondal, Debarshi Roy, Juliana Camacho Pereira, Eduardo N. Chini, Vijayalakshmi Shridhar
Journal: Cancer Research (2014)

Abstract We have previously shown that loss of HSulf-1, an endosulfatase that removes 6-O sulfation of HSPGs augmented heparin binding growth factor (HB-GF) signaling resulting in enhanced tumorigenesis, angiogenesis and metastasis in ovarian cancer. However, the role of HSulf-1 in energy metabolism and how the increased growth factor signaling in cells with loss of HSulf-1 altered cellular metabolism in cancer yet to be investigated. Here, we show for the first time that batch clonal lines of HSulf-1 knockdown cells [OV202 (Sh1/2)] and HSulf-1 knock out (KO) MEFs showed a significantly higher glycolytic flux (glucose uptake and lactate production) and reduced oxygen consumption rate (OCR) compared to a non-transduced control (OV202NTC) and wild-type (WT) MEF cells respectively. Conversely, enhanced expression of HSulf-1 in TOV21G cells resulted in lower glycolytic flux and higher OCR. Microarray analysis revealed that several of the genes in the glycolytic pathway such as Glut1, HK2, PFKFB3, ALDOC, PGAM, PKM2 and LDHA were upregulated in Sh1/2, cells compared to NTC cells. Expression of these genes and their respective products were validated both at the mRNA and protein levels in OV202Sh1/2, TOV21G Vec and MEFs KO cells compared to OV202NTC, TOV21G Cl13 and MEF WT respectively. Importantly, we found that loss of HSulf-1 decouples the energy flow from glycolysis to mitochondria by lowering the PDH and up-regulating PDK1 expression. We further demonstrated that loss of HSulf-1 reduced the total cellular ATP levels in Sh1/Sh2, TOV21G VEC, and MEFs KO cells, all consistent with lower mitochondrial activity as evidenced by decreased total number of mitochondria, its cross sectional area and perimeter in Sh1/Sh2, TOV21G Vec and MEF KO cells. This augmented aerobic glycolysis with loss of HSulf-1 was further corroborated by metabolomics study which showed significantly higher glycolytic metabolites such as G-6P, F1,6BP, F2,6BP, 3PG. While most of the TCA intermediates were downregulated in Sh1 and Sh2 cells; glutamate, glutamine, α-ketoglutarate and 2-hydroxyglutarate were higher upon loss of HSulf-1. These results suggests that loss of HSulf-1 plays a pivotal role in reprogramming the energy metabolism towards the Warburg effect which are critical to sustain cancer cells proliferation, transformation and angiogenesis. Citation Format: Susmita Mondal, Debarshi Roy, Juliana Camacho Pereira, Eduardo N. Chini, Vijayalakshmi Shridhar. Loss of sulf-1 reprograms energy metabolism in ovarian cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3360. doi:10.1158/1538-7445.AM2014-3360
The influence of enzyme detergent on skin reactions to beryllium
Authors: F.RAY BETTLEY, ALAN O'SHEA
Journal: British Journal of Dermatology (1974)

Journal Article The influence of enzyme detergent on skin reactions to beryllium Get access F.RAY BETTLEY, F.RAY BETTLEY The Institute of Clinical Research, The Middlesex Hospital, London Search for other works by this author on: Oxford Academic Google Scholar ALAN O'SHEA ALAN O'SHEA The Institute of Clinical Research, The Middlesex Hospital, London Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 91, Issue 1, 1 July 1974, Pages 33–38, https://doi.org/10.1111/j.1365-2133.1974.tb06714.x Published: 01 July 1974 Article history Accepted: 25 October 1973 Published: 01 July 1974
Molecular, structural, and immunologic relationships between different families of recombinant calcium-binding pollen allergens
Authors: Raffaella Tinghino, Anna Twardosz, Bianca Barletta, Eleonora M.R. Puggioni, Patrizia Iacovacci, Cinzia Butteroni, Claudia Afferni, Adriano Mari, Brigitte Hayek, Gabriella Di Felice, Margarete Focke, Kerstin Westritschnig, Rudolf Valenta, Carlo Pini
Journal: Journal of Allergy and Clinical Immunology (2002)
Mismatch on Tc-99m DTPA Aerosol Ventilation-Perfusion Lung Scan Caused by Achalasia
Authors: ROBERT R. BUTLER, L. HABELSON WILF
Journal: Clinical Nuclear Medicine (1994)

Tc-99m DTPA aerosol ventilation in conjunction with Tc-99m MAA perfusion scintigraphy is widely used in the evaluation of pulmonary embolism. The authors describe a case that demonstrates the importance of comparing any ventilation-perfusion study to the chest radiograph and clinical history to accurately evaluate the probability of pulmonary emboli.
Child-Resistant Containers and the Elderly
Authors: Matilda S. McIntire
Journal: JAMA (1979)

<h3>To the Editor.—</h3> We applaud the comments by Sherman et al (241:1001, 1979) on the problem of child-resistant containers for the elderly. However, while 33% of those persons older than 60 years had difficulty, only 9% discontinued use of the product, vs 15% for those 31 to 60 years and 20% younger than 30 years. This emphasizes the need for information to the elderly and testing their ability to use child-resistant containers properly. We strongly endorse the authors' suggestion that all health professionals caring for the elderly should demonstrate the container's proper use and that the patient's ability to comply should be tested. Further technological improvement in safety caps is also suggested.
Availability of polychlorinated biphenyls (PCBs) and lindane for uptake by intestinal Caco-2 cells.
Authors: A G Oomen, J Tolls, M Kruidenier, S S Bosgra, A J Sips, J P Groten
Journal: Environmental Health Perspectives (2001)

Children may ingest contaminated soil from hand to mouth. To assess this exposure route, we need to know the oral bioavailability of the contaminants. Two determining steps in bioavailability of soil-borne contaminants are mobilization from soil during digestion, which is followed by intestinal absorption. The first step has been investigated in previous studies that showed that a substantial fraction of PCBs and lindane is mobilized from soil during artificial digestion. Furthermore, almost all contaminants are sorbed to constituents of artificial human small intestinal fluid (i.e., chyme), whereas only a small fraction is freely dissolved. In this study, we examine the second step using intestinal epithelial Caco-2 cells. The composition of the apical exposure medium was varied by addition of artificial chyme, bile, or oleic acid at similar or increasing total contaminant concentrations. The uptake curves were described by rate constants. The uptake flux seemed to be dose-dependent. Furthermore, different exposure media with similar total contaminant concentrations resulted in various uptake rates. This can be attributed to different freely dissolved concentrations and carrier effects. In addition, the large fractions of contaminants in the cells indicate that PCBs and lindane sorbed to bile, oleic acid, and digestive proteins contributed to the uptake flux toward the cells. These results can be extrapolated qualitatively to in vivo conditions. Because the sorbed contaminants should be considered available for absorption, the first step of mobilization from soil is the most important step for oral bioavailability of the presently investigated soil-borne contaminants.
C-Reactive Protein Colocalizes With Complement in Human Hearts During Acute Myocardial Infarction
Authors: Wim K. Lagrand, Hans W.M. Niessen, Gert-Jan Wolbink, Lies H. Jaspars, Cees A. Visser, Freek W.A. Verheugt, Chris J.L.M. Meijer, C. Erik Hack
Journal: Circulation (1997)

Background Rises in circulating C-reactive protein (CRP), the prototypical acute-phase protein in humans, correlate with clinical outcome in patients with myocardial ischemia and infarction. We hypothesized that these correlations might reflect active participation of CRP in the local inflammatory response ensuing in the jeopardized myocardium because on binding to a ligand, CRP is able to activate the classic pathway of complement, and in addition, complement activation has been shown to occur locally in infarcted myocardium. Methods and Results To verify our hypothesis, we investigated localization of CRP in relation to deposition of complement in tissue specimens of infarcted and healthy heart tissue obtained from 17 patients who had died after acute myocardial infarction. CRP was found to be deposited only in infarcted regions and not in normal-appearing areas of the myocardium, being colocalized with depositions of C4 and C3 activation fragments of the complement system. Deposition of CRP and complement in infarcted myocardium appeared to be time dependent, because it was found in all infarctions except for one of young age (&lt;12 hours old) and two of greater age (&gt;1 year old), whereas another tissue specimen of an infarct &lt;12 hours old showed only moderate but positive staining for both CRP and complement in comparison with older infarctions. Conclusions We conclude that in humans, CRP may localize in infarcted heart tissue and suggest that this acute-phase protein promotes local complement activation, and hence tissue damage, in acute myocardial infarction.
Aminophylline and Chronic Obstructive Pulmonary Disease
Authors: DAWNA GILCHRIST
Journal: Annals of Internal Medicine (1988)

Letters and Corrections1 March 1988Aminophylline and Chronic Obstructive Pulmonary DiseaseDAWNA GILCHRIST, M.D., CHARLES K. CHAN, M.D.DAWNA GILCHRIST, M.D.Search for more papers by this author, CHARLES K. CHAN, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-108-3-488_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: Rice and associates (1) have done a well-designed, rigorous clinical trial on one of the common uses of aminophylline. The authors address the question of whether a widely used treatment strategy in acute exacerbation of chronic obstructive pulmonary disease is efficacious. According to their conclusions, supported by an analysis of power of 0.95, we should consider significantly altering our approach. However, we are reluctant to do so on the basis of a trial that includes only 28 patients.We would like to know how the authors arrived at their power value of 0.95. Specifically, which outcome variables...References1. RICELEATHERMANDUANE KJP. Aminophylline for acute exacerbations of chronic obstructive pulmonary disease: a controlled trial. Ann Intern Med. 1987;107:305-9. LinkGoogle Scholar2. MEINERT C. Sample size and power estimates. In: MEINERT CL, ed. Clinical Trials, Design, Conduct, and Analysis. New York: Oxford University Press; 1986: 71-89. CrossrefGoogle Scholar1. DAVIES O. Table E. In: Davies OL, ed. The Design and Analysis of Industrial Experiments. Edinburgh: Oliver and Boyd; 1956. Google Scholar2. EATONMACDONALDCHURCHNIEWOEHNER MFTD. Effects of theophylline on breathlessness and exercise tolerance in patients with chronic airflow obstruction. Chest. 1982;82:538-42. CrossrefMedlineGoogle Scholar3. RICEKRONENBERGHEDEMARKNIEWOEHNER KRLD. Effects of chronic administration of codeine and promethazine on breathlessness and exercise tolerance in patients with chronic airflow obstruction. Br J Dis Chest. 1987;81:287-92. CrossrefMedlineGoogle Scholar4. MC GAVINARTVINLINAOEMCHARDY CMHG. Dyspnoea, disability and distance walked: comparison of estimates of exercise performance in respiratory disease. Br Med J. 1978;2:241-3. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: University of Toronto The Wellesley Hospital Toronto, Ontario M4Y 1J3 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 March 1988Volume 108, Issue 3Page: 488-488KeywordsChronic obstructive pulmonary diseaseClinical trials Issue Published: 1 March 1988 PDF DownloadLoading ...
Amiodarone and Pneumonitis
Authors: MICHAEL DAN
Journal: Annals of Internal Medicine (1983)

Letters and Corrections1 November 1983Amiodarone and PneumonitisMICHAEL DAN, M.D., JOEL GREIF, M.D.MICHAEL DAN, M.D.Search for more papers by this author, JOEL GREIF, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-99-5-732_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: The question of whether amiodarone-induced pneumonitis should be treated with corticosteroids will be resolved only by a controlled trial comparing steroid treatment with placebo. Until then, the sole source of information is cumulative experience with scattered patients. Our recent experience with a patient with amiodarone-induced pneumonitis does not support the suggestion made by Dake and associates (1) that steroid treatment for this disorder may not be needed.A 63-year old woman was maintained on amiodarone, 200 mg/d, to prevent paroxysmal atrial fibrillation. After 3 years of therapy, she began to develop increasing fatigue, mild dyspnea...References1. DAKEGOLDEN MJ. Amiodarone and pulmonary effects [Letter]. Ann Intern Med. 1983;98:1028. LinkGoogle Scholar2. MARCHLINSKIGANSLERWAXMANJOSEPHSON FTHM. Amiodarone pulmonary toxicity. Ann Intern Med. 1982;97:938-45. LinkGoogle Scholar3. HUNNINGHAKEGADEKKAWANAMIFERRENSCRYSTAL GJOVA. Inflammatory and immune processes in the human lung in health and disease: evaluation by bronchoalveolar lavage. Am J Pathol. 1979;97:149-206. MedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Tel Aviv Medical Center Tel Aviv Israel PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byAmiodarone-induced pulmonary toxicity mimicking acute pulmonary edemaDRUG-INDUCED PLEURAL DISEASEBronchoalveolar Lavage Cell Data in Amiodarone-Associated PneumonitisAmiodarone Pulmonary ToxicityDrug-induced pulmonary disease 1 November 1983Volume 99, Issue 5Page: 732-732KeywordsAtrial fibrillationDyspneaFatiguePneumonitis Issue Published: 1 November 1983 PDF downloadLoading ...
Association between obesity and asthma in a twin cohort
Authors: S. F. Thomsen, C. S. Ulrik, K. O. Kyvik, T. I. A. Sørensen, D. Posthuma, L. R. Skadhauge, I. Steffensen, V. Backer
Journal: Allergy (2007)

Background: Obesity is linked to asthma in a yet poorly understood manner. We examined the relationship between obesity and asthma in a population‐based sample of twins. Methods: From the cohorts born between 1953 and 1982, who were enrolled in The Danish Twin Registry, a total of 29 183 twin individuals participated in a nationwide questionnaire study, where data on height, weight and asthma were collected. Latent factor models of genetic and environmental effects were fitted using maximum likelihood methods. Results: The age‐adjusted risk of asthma was increased both in obese females, OR = 1.96 (1.45–2.64), P ≤ 0.001 and in obese males, OR = 1.59 (1.08–2.33), P = 0.02. According to best‐fitting models, the heritability for obesity was 81% in males and 92% in females, whereas the heritability for asthma was 78% and 68% in males and females respectively. The age‐adjusted genetic liabilities to obesity and asthma were significantly correlated only in females, r = 0.28 (0.16–0.38). Conclusions: Obese subjects have an increased risk for asthma, which in females seems partly because of common genes.
UK considers banning the export to the US of three execution drugs
Authors: C. Dyer
Journal: BMJ (2011)

The UK government is urgently considering putting export controls on three drugs used to execute death row prisoners in the US, amid fears that a prisoner may have died an “excruciating” death after being inadequately anaesthetised with drugs imported from the UK.

Tom Smith, head of export control at the Department for Business, Innovation and Skills, told a hearing at the Houses of Parliament in London that the UK was urgently looking at controlling the export of pentobarbital, pancuronium bromide, and potassium chloride, three drugs used in execution protocols in the US.

Controls were placed last November on thiopental sodium, the drug usually used to …
Increases in Creatine Kinase After Exercise in Patients Treated With HMG Co-A Reductase Inhibitors
Authors: P. D. Thompson, A. M. Nugent, P. N. Herbert
Journal: JAMA (1990)

<h3>To the Editor.—</h3> Creatine kinase (CK) elevations of at least twice normal occurredin 11% of patients during clinical trials of lovastatin (J. H. Milander, MD, personal communication, 1989), and rhabdomyolysis with renal failure has occurred with lovastatin in combination with other medications.<sup>1</sup>Physical exertion can also injure muscle. Average CK levels over 3000 U/L have been noted in runners after a 42-km marathon.<sup>2</sup> We recently participated in a study using fluvastatin, a new 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase inhibitor, lovastatin, and placebo. Twenty-three men and 13 women received placebo for 6 weeks followed by 6 weeks of placebo or fluvastatin. Thirty-four patients subsequently received lovastatin. Four men receiving reductase inhibitors developed elevated CK levels after exercise, suggesting that these drugs exacerbate the muscle injury associated with exertion (Table). Patient 1 (Table) began jogging and weight lifting several days before phlebotomy. Patient 2 played squash for
DERMATITIS DUE TO DEXTRINS USED AS AN ADHESIVE ON TAX STAMPS
Authors: J. D. Walters
Journal: JAMA (1941)

It is a fact that dextrin products used in numerous occupations, such as paper hanging and bookbinding, can often produce a dermatitis. What is not so well known, however, is that dextrin appearing on the adherent surfaces of stamps or labels can have the same effect. There are establishments in which employees affix tax stamps, by hand, to cigaret packages. Some of these workers do not have the opportunity to change the stamp water as frequently as it should be done. The continuous contact of the hands (and fingers) with the dextrin in the stamp water and with the cloths used to dry the excess fluid off the packages sometimes produces a dermatitis. Two such cases have come to our attention. <h3>REPORT OF CASES</h3><h3>Case 1.—</h3> M. P., a woman aged 26, was employed in March 1938 to affix tax stamps. Two months later a dermatitis appeared on the palmar
Compensating Physicians for Telephone Calls-Reply
Authors: Paul Clay Sorum
Journal: JAMA (1995)

<h3>In Reply.</h3> —I share Dr Balon's annoyance at the time spent talking with third-party payers. Nonetheless, it is important conceptually and strategically to keep the two issues separate. The case in favor of compensating physicians for telephone encounters with patients is broader and stronger than that for calls with third-party payers. Greater medical judgment and risk are involved in calls with patients. Furthermore, compensating for calls with patients would result in more efficient medical care, more timely follow-up, and better documentation of telephone encounters. The computerized patient record of Dr Churgin provides important data on the frequency of telephone encounters in primary care. Moreover, this system will enable them to document the impact on the numbers of telephone and face-to-face encounters if their HMO decides to count telephone encounters when measuring productivity. I appreciate Dr Heath's support. He and Dr Spitz point out that lawyers charge for telephone calls. This
Public–Private Partnerships and International Health Policy-making: How can Public Interests be Safeguarded?
Authors: Andrew Herxheimer
Journal: Journal of the Royal Society of Medicine (2005)

The concept of public-private partnerships (PPPs) became prominent in the Reagan-Thatcher era. It grew from the notion that hierarchical bureaucracies are inefficient and that market mechanisms could make them more efficient, and that politicians and bureaucrats do not always act in the public interest, but may pursue either their own interests or those of powerful interest groups. The first PPPs were local and national, but since the turn of the century many international ones have developed, including major PPPs concerned with public health. Judith Richter's compact review examines the effects of these partnerships on international health policies, and particularly on WHO.
Increasing the sensitivity of the rodent uterotrophic assay to estrogens, with particular reference to bisphenol A.
Authors: J Ashby
Journal: Environmental Health Perspectives (2001)

The gravimetric uterotrophic assay is currently the most well-established, short-term rodent estrogenicity assay. Increasing attention is being paid to the extent to which use of morphometric or molecular changes in the uterus could act as surrogates for the gravimetric end point of the assay, thereby perhaps increasing the sensitivity of the assay. In this paper I discuss the available data, paying particular attention to studies on bisphenol A (BPA) because it offers the largest database for consideration. I conclude that the case has yet to be made for augmenting the gravimetric end point of the uterotrophic assay. To resolve this important question, it will be necessary to conduct detailed dose-response studies where the no-observed-effect level (NOEL) for the proposed surrogate end points are compared with the NOEL for the gravimetric end point. Currently, few such studies exist, and among those that do no clear message emerges. The general trend to increasing use of molecular assays in toxicology (multigene microarrays and real-time polymerase chain reaction) emphasizes the need for clear criteria for comparing the performance of individual markers of toxicity.
The Normal Child: Some Problems of the First Five Years and Their Treatment
Authors: Marietta M. Henry
Journal: JAMA (1969)

Normal children fare poorly in this day of enzyme chemistry and exotic syndromes. But they cannot be ignored. Mothers' questions about normal children and about child-rearing must still be answered by their physicians. This English book, very worthwhile, is indeed a treasury of practical answers to common pediatric problems. Breast feeding, rarely taught in current medical education, finds a welcome place here, and in several chapters, the author discusses the problems and mechanics of successful breast feeding. He presents the developmental milestones of infancy and childhood in thorough form, dwelling upon the individual variation so often found. His chapters concerning personality development and discipline contain wit and humor and also provide a firm philosophy of child-rearing. Preparation and selection of formulas are oriented toward English brand names, but that chapter is a minor bit of provincialism in an otherwise universally applicable book.