tbuckley/cabot-search huggingface dataset:
Anti‐annexin V antibody in systemic lupus erythematosus patients with lupus anticoagulant and/or anticardiolipin antibody
Authors: Juzo Matsuda, Noriko Saitoh, Kengo Gohchi, Moritaka Gotoh, And Miyo Tsukamoto
Journal: American Journal of Hematology (1994)

Abstract We investigated anti‐annexin V antibody (aANX) in patients with systemic lupus erythe‐matosus (SLE), and correlated to positivity with lupus anticoagulant (LA)/anticardiolipin antibody (aCL). aANX was positive in 12/47 SLE patients (26%), including 7 with β 2 ‐glycoprotein 1 (GPl)‐dependent aANX. The positivity of aANX was higher in patients with aCL (19%) and LA/aCL (50%) than in those without LA/aCL (10%). From these results, it is concluded that aANX is an autoantibody closely related to LA/aCL, and can be a possible new risk marker for thrombosis. © 1994 Wiley‐Liss, Inc.
HLA-matched sibling transplantation with G-CSF mobilized PBSCs and BM decreases GVHD in adult patients with severe aplastic anemia
Authors: Sun Zi-Min, Liu Hui-Lan, Geng Liang-Quan, Wang Xin-Bing, Yao Wen, Liu Xin, Ding Kai-Yang, Han Yong-Sheng, Yang Hui-Zhi, Tang Bo-lin, Tong Juan, Zhu Wei-Bo, Wang Zu-Yi
Journal: Journal of Hematology & Oncology (2010)

Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is an effective treatment for severe aplastic anemia (SAA). However, graft failure and graft-versus-host disease (GVHD) are major causes of the early morbidity in Allo-HSCT.To reduce graft failure and GVHD, we treated fifteen patients with SAA using high- dose of HSCT with both G-CSF mobilized PB and BMSCs from HLA-identical siblings to treat patients with SAA.All patients had successful bone marrow engraftment. Only one patient had late rejection. Median time to ANC greater than 0.5 × 10(9)/L and platelet counts greater than 20 × 10(9)/L was 12 and 16.5 days, respectively. No acute GVHD was observed. The incidence of chronic GVHD was 6.67%. The total three-year probability of disease-free survival was 79.8%.HSCT with both G-CSF mobilized PB and BMSCs is a promising approach for heavily transfused and/or allo-immunized patients with SAA.
Discovery of active enhancers through bidirectional expression of short transcripts
Authors: Michael F Melgar, Francis S Collins, Praveen Sethupathy
Journal: Genome biology (2011)

Long-range regulatory elements, such as enhancers, exert substantial control over tissue-specific gene expression patterns. Genome-wide discovery of functional enhancers in different cell types is important for our understanding of genome function as well as human disease etiology. In this study, we developed an in silico approach to model the previously reported phenomenon of transcriptional pausing, accompanied by divergent transcription, at active promoters. We then used this model for large-scale prediction of non-promoter-associated bidirectional expression of short transcripts. Our predictions were significantly enriched for DNase hypersensitive sites, histone H3 lysine 27 acetylation (H3K27ac), and other chromatin marks associated with active rather than poised or repressed enhancers. We also detected modest bidirectional expression at binding sites of the CCCTC-factor (CTCF) genome-wide, particularly those that overlap H3K27ac. Our findings indicate that the signature of bidirectional expression of short transcripts, learned from promoter-proximal transcriptional pausing, can be used to predict active long-range regulatory elements genome-wide, likely due in part to specific association of RNA polymerase with enhancer regions.
FIP1L1-PDGFRα D842V, a novel panresistant mutant, emerging after treatment of FIP1L1-PDGFRα T674I eosinophilic leukemia with single agent sorafenib
Authors: E Lierman, L Michaux, E Beullens, P Pierre, P Marynen, J Cools, P Vandenberghe
Journal: Leukemia (2009)
Dutch GPs call for ban on Novartis products
Authors: T. Sheldon
Journal: BMJ (2002)

Three Dutch GPs are calling on doctors not to prescribe Novartis products in protest at the Swiss based pharmaceutical company's “information campaign” urging people with onychomycosis, or ringworm of the nails, to visit their GP.

In a row reflecting concerns across Europe, Dutch doctors and the government's advertising code commission had criticised the Novartis campaign, claiming that it promotes the company's prescription only product terbinafine (Lamisil). The Dutch Society of General Practitioners shares the concern that such information campaigns are an unnecessary burden, threatening the efficiency of prescribing and focusing attention on unimportant …
Rheumatic Heart Disease From Ages 71 to 98
Authors: James H. Currens
Journal: JAMA (1967)

THE NATURAL HISTORY of any chronic disease is always a subject of interest and importance to the clinician. Unusual cases, therefore, deserve recording when they represent or illustrate the exception that also tends to alter or broaden the rule.<sup>1</sup> <h3>Report of Cases</h3> The two cases reported here illustrate the course that rheumatic heart disease may take in the aged. <h3>Case 1.—</h3> A 46-year-old single, white woman was first examined in 1933 for questionable anemia. The family history indicated that her father died of a stroke at age 70 and her mother died of stomach ulcers at age 69. Two sisters were living and well, although one had rheumatic fever and nephritis at age 43 following severe tonsillitis. The sister's blood pressure was also elevated. There were no brothers. The patient gave no history of scarlet fever, rheumatic fever, or chorea but there was a history of recurrent tonsillitis prior
Abstract 1613: Delta-tocotrienol delays the progression of pancreatic intraepithelial neoplasia (PanIN) lesions in a conditional KrasG12D mouse model
Authors: Kazim Husain, Barbara Centeno, Marta Perez, Guo Lee, Sabiha Kazim, Dung-Tsa Chen, Said Sebti, Mokenge Malafa
Journal: Cancer Research (2012)

Abstract Background: We have shown that natural vitamin E γ-tocotrienol is the most bioactive tocotrienols in inhibiting the cell proliferation and induction of apoptosis in pancreatic cancer cells as well as in xenograft models. Therefore in this study we evaluated the chronic 12-month feeding of γ-tocotrienol on the prevention of the progression of PanIN lesions in a conditional KrasG12D mouse model. Methods: Offspring of LSLKRASG12D x PDX-1-Cre intercrosses (double positive by genotyping) were randomized to three groups: 1) No treatment (NT), 2) Vehicle (ethanol extracted olive oil, 1.0 ml/kg x 2/ day, PO) and 3) γ-tocotrienol (200 mg/kg x 2/day, PO). The treatment was started at the age of 10 weeks and continued for 12 months. The survival of the mice in three groups was plotted by Kaplan-Meir graph. The pancreas of KRASG12D; PDX-1-Cre mice was analyzed for the presence of murine PanINs. The apoptosis, cell cycle and signaling markers were determined by Western blotting and immunostaining. Results: No significant difference in food intake and body weight gain between γ-tocotrienol and control groups was observed during 12 months feeding. γ-tocotrienol treatment significantly increased survival of mice compared to control groups (p&amp;lt;0.025). Mice fed γ-tocotrienol had a significant delay in the progression of mPanIN lesions. The proportion of mPanIN-3 lesion was significantly decreased (P&amp;lt;0.001), whereas the relative numbers of all other lesions combined were slightly increased in the γ-tocotrienol-fed animals. γ-tocotrienol feeding also inhibited the pMEK and pERK signaling and increased p27Kip1 expression in the pancreatic tumor tissues. The apoptosis markers such as caspase-3, PARP1 cleavage, and Bax expression were increased in tumor tissues of mice treated with γ-tocotrienol compared to controls. NF-κB/p65 and the NF-κB transcriptional targets Bcl-XL was down-regulated by γ-tocotrienol treatment. The ratio of the proapoptotic (Bax) and antiapoptotic (Bcl-XL) proteins was increased almost 4-folds in the pancreatic tumor tissues compared to controls. Conclusion: γ-tocotrienol delays the progression of pancreatic cancer precursor lesions in a preclinical animal model hence warrant it's clinical use for the prevention and treatment of pancreatic cancer in high-risk patients. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 1613. doi:1538-7445.AM2012-1613
Lymphangiography
Authors: James F. Crispen, Peter F. Jeffries
Journal: JAMA (1962)

LYMPHANGIOGRAPHY is a method which permits visualization of the lymphatic system by the injection of radiopaque material directly into a lymphatic vessel. Information gained by such studies has diagnostic, therapeutic, and prognostic value, as well as interest to the anatomist and physiologist. Although great strides have been made in the past decade in this field, the true value of lymphangiography is merely "suggested," as it is still in its infancy and available to clinicians in only a few centers. It is our hope that the method outlined here will lead to a more widespread use of this valuable adjunct in the evaluation of certain patients. The early studies in animals and cadavers were conducted in the 1930's, and are well outlined by Sheehan et al., and Gergel. Kinmoth et al., in 1952, initiated the modern technique of direct lymphangiography in living humans. They suggested its clinical usefulness and demonstrated incompetency
In vivo comparison of cerebral tissue PO2 and cytochrome aa3 reduction-oxidation state in cats during hemorrhagic shock.
Authors: K Kariman, F G Hempel, F F Jöbsis, S R Burns, H A Saltzman
Journal: Journal of Clinical Investigation (1981)

To assess the adequacy of oxygen availability and utilization within the cerebral cortex in vivo, we have measured the partial pressure of oxygen in tissue (PtO2), as well as the reduction oxidation state of cytochrome c oxidase (cyt aa3) during shock induced by slow or rapid hemorrhage in anesthetized cats. PtO2 was measured with pyrenebutyric acid-generated fluorescence in cerebral cortical cells. Cyt aa3 redox state was measured by the absorption of monochromatic light at 605 nm absorption peak of the enzyme reflected from the same cortical field. The PtO2 remained within the normal range until either 30 +/- 1.5 ml blood/kg was removed or the mean arterial pressure fell by 70 +/- 5% of base line. Beyond either point, the PtO2 fell rapidly to a low value approximating zero. By contrast, the reduction of cyt aa3 began early when as little as 5 ml blood/kg was removed. Thereafter, the shift toward reduction was progressive and continuous with a slow rate at first and a rapid rate later. This accelerated rate of cyt aa3 reduction preceded the rapid fall of PtO2. We concluded that, under these experimental conditions, cyt aa3 reduction is a much earlier and more sensitive indicator of perturbed intracellular aerobic metabolism due to hemorrhage that is PtO2.
BLOOD PLASMA PROTEIN PRODUCTION AND UTILIZATION
Authors: S. C. Madden, C. A. Finch, W. G. Swalbach, G. H. Whipple
Journal: The Journal of Experimental Medicine (1940)

When blood plasma proteins are depleted by bleeding with return of the washed red blood cells (plasmapheresis) it is possible to bring dogs to a steady state of hypoproteinemia and a uniform plasma protein production on a basal low protein diet. These dogs are clinically normal. Introduction of variables into their standardized life gives insight into the production of plasma protein. Casein retested as the basal protein in the ration may show high yield of plasma protein, equal to 33 per cent of the protein fed. This equals the potency of liver protein (17 to 33 per cent) and approaches the utilization of plasma protein by mouth (40 per cent). Zein has no effect upon plasma protein regeneration but when it is supplemented with cystine, tryptophane, lysine, and glycine, there is a doubling of the liver basal plasma protein production and a retention of the fed protein nitrogen. Threonine does not modify the above reaction. Liver protein supplemented with cystine, leucine, glutamic acid, and glycine in the basal diet yields double the amount of new formed plasma protein compared with liver alone. This combination is then as potent as plasma protein itself when given by mouth-40 per cent utilization. Tyrosine or lysine, arginine, and isoleucine do not modify the above responses. Methionine is not as effective as cystine in supplementing gelatin and tyrosine to produce plasma protein. Cystine, leucine, and glutamic acid appear to be of primary importance in the building of new plasma protein in these experiments. Plasma protein formation is dependent upon materials coming from the body reserve and from the diet. Given an exhaustion of the reserve store there is very little plasma protein produced during a protein fast (3 to 6 gm. per week). A turpentine abscess does not modify this fasting plasma protein reaction. Homologous plasma given by vein will promptly correct experimental hypoproteinemia due to bleeding. It will maintain nitrogen equilibrium and replenish protein stores. Even during hypoproteinemia plasma protein may promptly pass out of the circulation to supply body needs for protein. Perhaps the most significant concept which derives from all these experiments is the fluidity of the body protein (including plasma protein)-a ready give and take between the protein depots-a "dynamic equilibrium" of body protein.
Fluid Resuscitation After Major Burns
Authors: Robert H. Demling
Journal: JAMA (1983)

IN THE 1940s, hypovolemic shock in the early postburn period was the leading cause of death after severe burn injury. Today, with our current knowledge of the massive fluid shifts and vascular changes that occur, early mortality has decreased considerably. The failure rate for adequate volume restoration is less than 5%, even for deep burns involving up to 85% of the total body skin surface.<sup>1</sup>The problem of nonreversible shock has now been replaced by that of generalized burn edema formation, a problem clearly less lethal but one that can result in serious morbidity. Tissue ischemia due to an increase in interstitial pressure is one complication of edema.<sup>2</sup>Pulmonary insufficiency, as a result of both a decrease in chest wall compliance from tissue edema and an increase in blood volume during edema mobilization, is another potential complication. Hypovolemia can be corrected and edema at least partially controlled, however,
Premature expression of the macrophage colony-stimulating factor receptor on a multipotential stem cell line does not alter differentiation lineages controlled by stromal cells used for coculture.
Authors: T Kinashi, K H Lee, M Ogawa, K Tohyama, K Tashiro, R Fukunaga, S Nagata, T Honjo
Journal: The Journal of Experimental Medicine (1991)

We are interested to know whether expression of a lineage-specific growth factor receptor is deterministic to lineage commitment during hematopoiesis. For this purpose, we introduced the human c-fms gene into the multipotential stem cell clone LyD9 and two myeloid progenitor clones, L-GM3 and L-G3, cells that differentiate in response to granulocyte/macrophage colony-stimulating factor (GM-CSF) and granulocyte (G)-CSF, respectively. Although LyD9 cells have differentiation potential to become macrophages, c-fms transfectants of LyD9 and L-GM3 cells did not differentiate in response to human macrophage (M)-CSF. However, c-fms transfectants of L-G3 cells differentiated to neutrophils in response to human M-CSF. These results indicate that the M-CSF receptor requires a specific signal transduction pathway to exert its differentiational and proliferative effects. Furthermore, the M-CSF receptor can convey a granulocyte-type differentiation signal possibly by cooperating with the G-CSF receptor signal transduction pathway. The c-fms-transfected LyD9 cells as well as the original LyD9 cells differentiated predominantly into GM-CSF- and G-CSF-responsive cells by coculturing with PA6 and ST2 stromal cells, respectively. The results indicate that differentiation lineage is not affected by premature expression of the M-CSF receptor. Instead, the stromal cell used for coculture apparently controls lineage-selective differentiation of the multi-potential stem cell line.
Curling's Ulcer: An Experiment of Nature
Authors: Claude E. Welch
Journal: JAMA (1972)

Owen Wangensteen in his introduction to this monograph describes it as a "fascinating" work, and this reviewer will use exactly the same adjective, despite any charge of plagiarism. The authors describe in detail the galaxy of definitions, causes, contributions of experimental surgery, and clinical problems that surround the syndrome of "Curling's ulcer." Not only are the facts presented, but the critical comments and philosophic peregrinations of the senior author combine to effect a clear and interesting perspective of this perplexing lesion. The authors would replace the term "Curling's ulcer" by "gastrointestinal ulceration following burns," and indicate that the site of bleeding and classification as either an ulcer or an erosion must be specified. Their clinical studies confirm the frequency of this disease, since it has been noted in about 25% of all patients. Prophylactic therapy to prevent serious complications therefore is necessary. In only about 1% of patients are the
Precordial electrocardiographic mapping. A technique to assess the efficacy of interventions designed to limit infarct size.
Authors: J E Muller, P R Maroko, E Braunwald
Journal: Circulation (1978)

THE QUANTITY OF MYOCARDIUM which becomes necrotic following coronary occlusion has been shown to influence both the acute and long-term conse- quences of myocardial infarction.1 2 Fortunately, ex- periments now indicate that the size of a myocardial infarct is not irrevocably determined immediately following a cor- onary occlusion, but can be altered substantially by a number of interventions.3-1-However, the clinical assess- ment of interventions designed to protect ischemic myocar- dium has posed considerable difficulty.Precordial electro- cardiographic mapping, including analysis of both the ST segment and the QRS complex, which has been developed over the past several years, is now being used in studies on patients with acute myocardial infarction.In this review evidence will be presented which indicates that electrocar- diographic mapping, when employed properly and with ap- propriate awareness of its limitations, can yield valid results and indicate whether or not an intervention modifies either the severity of ischemia itself or the eventual size of an in- farction. Epicardial Electrocardiographic MappingThe electrocardiogram has been used in the study of myocardial ischemia and infarction for half a century.In 1918, in an effort to establish the utility of the electrocardiogram as an aid in the diagnosis of myocardial infarction, Smith conducted a systematic study in dogs of the changes in the electrocardiogram following coronary occlusion.12
Immunotherapy and Asthma
Authors: J. Mark Fitzgerald
Journal: JAMA (1989)

<h3>To the Editor.—</h3> I note with interest the recent article<sup>1</sup>regarding the role of intravenous corticosteroids in status asthmaticus. The article makes a useful contribution in elucidating the appropriate dose and mode of administration of corticosteroids in acute asthma. Despite this, I think it would be appropriate for the authors to comment on the fact that at the time of entry to the study, 21% of the patients in the oral group as against 11% of the patients in the intravenous group were taking steroids (Table 1 of the article). I think this could introduce a bias in the study, as patients who had started therapy before randomization might have had a significant effect on outcome. In addition, the authors fail to mention a previous study that supports their hypothesis.<sup>2</sup>
THE IMMUNOLOGICAL SIGNIFICANCE OF COLOSTRUM
Authors: Theobald Smith
Journal: The Journal of Experimental Medicine (1930)

Renal lesions chiefly the result of obstructive processes were associated with intranuclear bodies in the epithelium of straight and convoluted tubules. In one animal these bodies were found in large numbers in otherwise still normal organs. The affected animals had been fed cow serum in place of colostrum.
Electrocardiographic Monitoring— Bad News and Good News
Authors: Samuel Vaisrub
Journal: JAMA (1975)

Unlike long-range surveillance of chronic diseases by periodic follow-ups, short-term monitoring of cardiac arrhythmias does not depend on a patient's compliance with therapy and his observance of return appointments, but on the observation of his rhythm disorders by a physician or nurse. Theoretically, such observation by trained professionals should be more reliable than compliance by a layman whose attitudes and capabilities are uncertain. But, apparently, it is not necessarily so. Prompted by the observation<sup>1</sup>that conventional monitoring in acute myocardial infarction often fails to detect transient rhythm disturbances, Lindsay and Bruckner (p 51) investigated 86 cases of proved or suspected myocardial infarction during 32 monitoring periods—a total of 754 patient-hours—in a coronary care unit. Heart beats were observed on an oscilloscopic display in the usual manner and were logged hourly by a team of three to five nurses. The logs were compared by the investigators with scans of electrocardiographic
Effects of zoloft® on platelet/endothelial biomarkers in depressed patients after acute coronary events: Sertraline Anti-Depressant Heart Attack Randomized Trial (SADHART platelet substudy)
Authors: Victor Serebruany, Alexander Glassman, Alex Malinin, Ranga Krishnan, Mitch Finkel, Charles Nemeroff, Paul Gurbel, Cathryn Clary, Wilma Harrison, Christopher O'Connor
Journal: Journal of the American College of Cardiology (2002)
AORTIC OBSTRUCTION SECONDARY TO NON-SPECIFIC AORTOARTERITIS IN CHILDREN MASQUERADING AS DILATED CARDIOMYOPATHY
Authors: Anita Saxena, Saurabh K. Gupta, Shyam Kothari, Rajneesh Juneja, S. Ramakrishnan, Sanjiv Sharma, Gurpreet Gulati
Journal: Journal of the American College of Cardiology (2013)
Why did it take 19 months to retrieve clinical trial data from a non-profit organisation?
Authors: A. Hrobjartsson
Journal: BMJ (2013)

Missing data in clinical trials is a major problem. One would expect the situation to be better for trials funded by large non-profit organisations that are free of commercial bias, and with policies for transparency and data sharing. However, in our experience with a non-profit trial funder we found this was not the case. Indeed, it was so bureaucratic that, despite the good intentions of the authors and others involved, accessing the data proved to be very difficult.

We wanted to study how treatment effects in randomised clinical trials were affected by a failure to blind the outcome assessors. We therefore systematically reviewed trials where the same outcome had been assessed by both a blind and a non-blind assessor within the same trial.1 2 One eligible study was a large, well performed trial of the effect of electrical deep brain stimulation for patients with advanced Parkinson disease, published in JAMA in January 2009.3 The trial was partially funded by the Cooperative Studies Program of the Department of Veterans Affairs’ Office of Research and Development. JAMA reported UPDRS motor scores (Unified Parkinson’s Disease Rating Scale III) evaluated by blind assessors, but did not …