tbuckley/cabot-search huggingface dataset:
Activation of mitogen-activated protein kinases and p90 ribosomal S6 kinase in failing human hearts with dilated cardiomyopathy
Authors: Y Takeishi
Journal: Cardiovascular Research (2002)

Objective: A new member of the MAP kinase family, big MAP kinase-1 (BMK1), has been recently identified to promote cell growth and attenuate apoptosis. P90 ribosomal S6 kinase (p90RSK), one of the potentially important substrates of extracellular signal regulated kinase (ERK), regulates gene expression in part via phosphorylation of CREB and the Na+/H+ exchanger. Recently, we have demonstrated that the activity of BMK1, Src (the upstream regulator of BMK1) and p90RSK was increased in hypertrophied myocardium induced by pressure-overload in the guinea pig. However, the abundance and activity of these kinases in human hearts are unknown. Methods: In addition to the three classical MAP kinases (ERK, p38 kinase, and c-Jun NH2-terminal kinase (JNK)), we examined the protein expression and activity of Src, BMK1, and p90RSK in explanted hearts from patients with dilated cardiomyopathy (n=9). Normal donor hearts, which were not suitable for transplant for technical reasons, were used as controls (n=5). Results: There were no significant differences in the levels of protein expression of these kinases between normal and failing hearts. ERK1/2 and p90RSK were activated in heart failure compared to control (P<0.01 and P<0.03, respectively), while the activity of p38 kinase was decreased (P<0.05) and the activity of JNK was unchanged in heart failure. By contrast, the activities of Src and BMK1 were significantly reduced in end-stage heart failure compared to normal donor hearts (P<0.05). Conclusion: These data suggest that multiple MAP kinases, p90RSK, and Src are differentially regulated in human failing myocardium of patients with idiopathic dilated cardiomyopathy and may be involved in the pathogenesis of this complex disease.
Russia is to crack down on sales of alcohol
Authors: A. Osborn
Journal: BMJ (2010)

Russia is to ban the sale of spirits in Moscow’s shops after 10 pm as part of the toughest anti-alcohol campaign since the fall of the Soviet Union.

The move, part of a government strategy to more than halve the nationwide consumption of alcohol in the next decade, will take effect on 1 September.

The law will make it illegal for the Russian capital’s supermarkets, shops, and kiosks to sell vodka and other spirits containing 15% or more of alcohol from 10 pm to 10 am. Current rules in Moscow ban the sale of vodka from 11 …
METABOLIZABLE IN-111 CHELATE CONJUGATED ANTI-IDIOTYPE MONOCLONAL ANTIBODY FOR RADIOIMMUNODETECTION OF LYMPHOMA IN MICE
Authors: Michael K. Haseman, David A. Goodwin, Claude F. Meares, Mark S. Kaminski, Theodore Wensel, Michael J. McCall, Ronald Levy
Journal: Clinical Nuclear Medicine (1984)

We investigated the relative biological properties of In-111 labeled monoclonal antibodies (MOAB) coupled with a conventional bifunctional chelate (BC) and with a new enzyme metabolizable bifunctional chelate (BCM). A rat IgG2A MOAB (7D4) against idiotype from a mouse B-cell lymphoma (38C-13) was chelate coupled then In-111 labeled just prior to use. C3H mice bearing 38C-13 flank tumors 100–500 mg in size were injected I.V. with labeled MOAB and imaged and/or sacrificed for organ counting at 24 or 48 hours. Control tumor mice were given In-111 chelate labeled rat anti-dinitrophenol IgG2A MOAB (DNP-BC). All tumors were clearly visualized with 7D4-BC and 7D4-BCM but not with DNP-BC. Organ distributions at 24 hours were as follows: % DOSE/GRAM TUMOR/BLOOD RATIO (TBR) 7D4-BC 7D4-BCM DNP-BC Blood 3.0 1.4 7.9 7D4-BC 1.8 Tumor 5.0 3.5 3.7 7D4-BCM 2.4* Liver 18.9 12.4 14.8 DNP-BC 0.5 Spleen 10.9 4.8 7.4 *(TBR's as high as 8.3 were seen at 48 hours) Kidneys 4.0 7.9 14.4 Use of BCM resulted in a substantial lowering of blood background, a shorter biological half life (43% excretion in 24 hours vs. 16%) and an increase in the TBR at the expense of a moderate decrease in absolute tumor uptake. The versatile chemistry of our C-1 substituted EDTA BC's provides for a variety of other possible enzyme cleavable groups (e.g., peptide bonds) for further investigation.
Exercise training improves left ventricular systolic function in older men.
Authors: A A Ehsani, T Ogawa, T R Miller, R J Spina, S M Jilka
Journal: Circulation (1991)

To determine whether endurance exercise training can improve left ventricular systolic function in older men, 10 healthy sedentary men (64 +/- 3 years old; mean +/- SD) were studied. Training consisted of endurance exercise 4 +/- 0.3 days per week for 11.8 +/- 2.5 months at a progressively increasing intensity of 60-80% of maximal O2 uptake (Vo2max) with additional brief bouts of exercise equal to 93 +/- 13% of Vo2max. Vo2max increased from 29.6 +/- 4.1 to 37.2 +/- 5.7 ml/kg/min (p less than 0.001). Percent body fat was decreased (17.8 +/- 3.6% versus 15.6 +/- 3.6%; p less than 0.001). Before training, left ventricular ejection fraction, determined by electrocardiographic-gated equilibrium blood pool imaging, increased only modestly during exercise (from 66.3 +/- 6.7% at rest to 70.6 +/- 6.9% at peak exercise). After training, the increase in ejection fraction during exercise was significantly greater (from 67 +/- 4.8% at rest to 77.6 +/- 7.5% at peak exercise) than that observed before training and was similar to that in young sedentary men (64 +/- 7% at rest versus 74 +/- 9% at peak exercise). Although the changes in systolic pressure from rest to exercise were similar, end-systolic volume decreased significantly at peak exercise after (51 +/- 12 versus 38 +/- 13 ml; p less than 0.005) but not before (46 +/- 8 versus 43 +/- 13 ml; p = NS) training with a shift in the end-systolic volume-systolic blood pressure relation to the left compatible with enhanced inotropic state.(ABSTRACT TRUNCATED AT 250 WORDS)
The Lymphatics: Diseases, Lymphography and Surgery
Authors: Edward A. Edwards
Journal: JAMA (1973)

Nowhere does one appreciate more the inadequacy of our art than when faced with a patient suffering from lymphedema, especially if it is of elephantiasic proportions. No person has done more than John Kinmonth in clarifying the pathology of lymphedema, thus giving a basis for the development of knowledge in this field. Lymphography, the method he developed, has become widely used not only in the study of lymphedema, but even more in the diagnosis of lymphatic malignancy and the evaluation of its treatment. Offshoots of lymphography include the identification of lymph nodes at operation through the injection of dyes, and endolymphatic therapy with radioactive isotopes. The present book is a more extensive report on his work than Kinmonth has previously published. Chapters on normal lymphographic appearances and on endolymphatic treatment are contributed by N. L. Browse, B. T. Jackson, and J. M. Edwards—the rest are written by Kinmonth himself. The
Chondroma of the Diaphragm - Report of a Case
Authors: M. Itakura, K. Shiraishi, T. Kadosaka, S. Matsuzaki
Journal: Endoscopy (1990)

A rare case of chondroma of the diaphragm is reported. The patient was a 23-year-old female who had had recurrent upper abdominal pain for 4 years. Her symptoms and physical findings on admission suggested an intra-abdominal tumor. Roentgenological study disclosed a mass with a thick calcified wall in the upper abdomen. Laparoscopy revealed a multinodular white mass protruding from the left hemidiaphragm. The mass was surgically enucleated from the diaphragm. The resected tumor had a thick, calcified wall and a narrow lumen containing colorless fluid. Microscopic examination disclosed a hyaline chondroid matrix with mature chondrocytes, as well as extensive ossification. Primary tumor of the diaphragm is an uncommon disease. Only one case of chondroma of the diaphragm has been reported in the literature. Laparoscopy was useful in confirming the tumor's location and in speculating on its nature.
Implications of Microscopic Satellites of the Primary and Extracapsular Lymph Node Spread in Patients With High-Risk Melanoma: Pathologic Corollary of Eastern Cooperative Oncology Group Trial E1690
Authors: U.N.M. Rao, J. Ibrahim, L.E. Flaherty, J. Richards, J.M. Kirkwood
Journal: Journal of Clinical Oncology (2002)

PURPOSE: To correlate the presence of extracapsular spread (ECS) of regional nodal metastases, and micrometastasis near the primary tumor, with disease outcome in the intergroup study E1690 in relation to the impact of recombinant interferon-alfa (rIFNα)-2b. PATIENTS AND METHODS: E1690 included 642 patients with American Joint Committee on Cancer stage IIB or III cutaneous melanoma. Patients were randomized into high- and low-dose rIFNα-2b treatment arms and an observation arm. Pathologic slides were reviewed for selected parameters from at least half of the subjects in all three arms. Evaluation of the primary tumor included notations regarding ulceration, mitotic activity, thickness, microscopic satellites (MS), and nodal ECS on a standardized pathology form. These data were collated in relation to relapse-free survival (RFS) and overall survival (OS) at 50 months’ follow-up and studied using Cox regression analysis. RESULTS: Ulceration, mitotic activity, thickness, and size of tumor-bearing lymph nodes did not show a statistically significant correlation with either OS or RFS across all treatment arms. The presence of MS was correlated with RFS (P = .0008) and OS (P = .05). ECS correlated with RFS (hazard ratio = 1.44, P = .032) but not OS (P = .11). CONCLUSION: The presence of MS (in 6% [18 of 308 patients]) had a significant adverse impact on both RFS (P = .0008) and OS (P = .053). Ulceration, mitotic activity, thickness, and number of positive lymph nodes had no significant effect on OS in this subset study (univariate or multivariate Cox analysis). The presence of ECS in lymph nodes had a significant adverse effect on RFS (P = .032) but not on OS.
Regulation of lipoprotein lipase in primary cultures of isolated human adipocytes.
Authors: P A Kern, S Marshall, R H Eckel
Journal: Journal of Clinical Investigation (1985)

To study the regulation of adipose tissue lipoprotein lipase (LPL) in human adipocytes, omental adipose tissue was obtained from healthy subjects and digested in collagenase. The isolated adipocytes thus obtained were suspended in Medium 199 and cultured at 37 degrees C. Cell viability was demonstrated in adipocytes cultured for up to 72 h by constancy of cell number, cell size, trypan-blue exclusion, and specific 125I-insulin binding. In addition, chloroquine induced an increase in cell-associated 125I-insulin at 24, 48, and 72 h after preparation. Thus, isolated adipocytes retained their ability to bind, internalize, and degrade insulin. LPL was measured as activity secreted into the culture medium (CM), released from cells by heparin (HR), and extracted from cell digests. A broad range of heparin concentrations produced a prompt release of LPL from a rapidly replenishable pool of cellular activity. When cells were cultured in medium containing 10% fetal bovine serum, there was a marked stimulation of CM and HR. The secretory response to serum (CM) correlated strongly with HR 24 h after preparation (rs = 0.731, P less than 0.001). In addition, HR was found to correlate logarithmically and inversely with body mass index (r = -0.731, P less than 0.001). Insulin, at 400 ng/ml only, increased HR by 36 +/- 10%, an effect simulated by lower concentrations of insulin-like growth factor-1 (IGF1). Thus, LPL is produced and regulated in isolated human adipocytes. The degree of adiposity and serum are important regulators of HR activity, whereas insulin is stimulatory only at a pharmacologic concentration. This effect of insulin may be mediated through the IGF1 receptor. Isolated human adipocytes represent a novel and useful system for the study of LPL and lipid metabolism as well as for other aspects of adipocyte biology.
Enzymes Might Cut Celiac Symptoms
Authors: Bridget M. Kuehn
Journal: JAMA (2006)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Interpretation of Biochemical Multitest Profiles: An Analysis of 100 Important Conditions
Authors: George D. Lundberg
Journal: JAMA (1977)

This is a simple, completely unpretentious book about interpretation of serum biochemical multitest profiles performed by two specific instruments, the SMA 12/60 and 6/60. Fundamentally the book consists of 100 case reports, with a copy of the strip chart in each instance. The 18 determinations are chloride, bicarbonate, potassium, sodium, urea nitrogen, glucose, total protein, albumin, calcium, inorganic phosphate, cholesterol, uric acid, creatinine, total bilirubin, alkaline phosphatase, creatine phosphokinase, lactate dehydrogenase, and aspartate transaminase. Each patient report includes a history (with physical examination), listing of laboratory results, analysis of the meaning of the results, appropriate references, and a depiction of the actual strip chart. The charts contain comprehensive, helpful information regarding normal (mean ±2 SD), adjacent ±3 SD zones, male-female differences, the most important causes of elevation and decrease, and other helpful notes. In short, there is a world of useful interpretive information on each one of Dr Wolf's famous
Indirubin-3′-monoxime exerts a dual mode of inhibition towards leukotriene-mediated vascular smooth muscle cell migration
Authors: Tina Blažević, Anja M. Schaible, Katharina Weinhäupl, Daniel Schachner, Felix Nikels, Christina Weinigel, Dagmar Barz, Atanas G. Atanasov, Carlo Pergola, Oliver Werz, Verena M. Dirsch, Elke H. Heiss
Journal: Cardiovascular Research (2013)

The small molecule indirubin-3'-monoxime (I3MO) has been shown to inhibit vascular smooth muscle cell (VSMC) proliferation and neointima formation in vivo. The influence of I3MO on VSMC migration and vascular inflammation, two additional key players during the onset of atherosclerosis and restenosis, should be investigated.We examined the influence of I3MO on VSMC migration, with focus on monocyte-derived leukotrienes (LTs) and platelet-derived growth factors (PDGFs) as elicitors. Exogenous LTB4 and cysteinyl leukotrienes as well as LT-enriched conditioned medium of activated primary human monocytes induced VSMC migration, which was inhibited by I3MO. I3MO also blunted migration of VSMC stimulated with the PDGF, the strongest motogen tested in this study. Induction of haem oxygenase 1 accounted for this anti-migratory activity of I3MO in VSMC. Notably, I3MO not only interfered with the migratory response in VSMC, but also suppressed the production of pro-migratory LT in monocytes. Conditioned media from monocytes that were activated in the presence of I3MO failed to induce VSMC migration. In cell-based and cell-free assays, I3MO selectively inhibited 5-lipoxygenase (5-LO), the key enzyme in LT biosynthesis, with an IC50 in the low micromolar range.Our study reveals a novel dual inhibitory mode of I3MO on LT-mediated VSMC migration: (i) I3MO interferes with pro-migratory signalling in VSMC and (ii) I3MO suppresses LT biosynthesis in monocytes by direct inhibition of 5-LO. These inhibitory actions on both migratory stimulus and response complement the previously demonstrated anti-proliferative properties of I3MO and may further promote I3MO as promising vasoprotective compound.
THE VITAL CAPACITY OF THE NEGRO CHILD
Authors: FRANK L. ROBERTS
Journal: JAMA (1927)

The purpose of this study is to ascertain the vital capacity of a group of normal negro children and compare these vital capacities with those of a group of white children living in the same territory. The negro child forms an integral part of our population and must be considered in any health program. It has been shown that negro children are heavier than white children of the same age and height,<sup>1</sup>and in many other ways the negro physical standard differs from that of the white. For this reason, therefore, it was thought advisable to determine the vital capacity of these children. In a careful review of the literature but little was found dealing with the vital capacity of negroes. Wilson and Edwards<sup>2</sup>measured thirty-eight normal colored children, and stated that these children had vital capacities definitely lower than white children. Dr. W. G. Smillie of the
Pilot study to examine the association between angiotensin-converting enzyme genotype and fluid retention in humans
Authors: G Evans, A Bevan, A Evans, T Kinsey, A Steel, J Ashworth
Journal: British Journal of Sports Medicine (2010)

Following ingestion of a variety of drinks, subsequent urine excretion in humans shows large variability.1 The reasons for this are currently unknown. Aldosterone, the end product of the renin angiotensin aldosterone system (RAAS), increases sodium and water re-absorption in the kidney. Circulating concentrations of angiotensin-converting enzyme (ACE), a key component of the RAAS, are affected by a polymorphism in the ACE gene involving the deletion (D) or insertion (I) of a 287 base pair sequence, with DD genotypes resulting in higher circulating concentrations of ACE.2 Following ethical approval, 29 healthy Caucasian males participated in this investigation. Participants arrived at the laboratory hydrated after an overnight fast. Following arrival, participants' height and body mass were measured before a 15-min supine rest period. Total body water was measured before a fingerprick blood sample was obtained in order to determine ACE genotype. Participants then provided a urine sample before ingesting 600 ml of a commercially available sports drink over a period of 5 min. A further urine sample was obtained 60 min later. Urine samples were analysed for osmolality and sodium concentration. The I allele (corresponding to either ID or II genotypes) was found in 12 participants and 17 participants were found not to have the I allele (corresponding to a DD genotype). Baseline characteristics of the two groups were the same (p&gt;0.05) with the exception of height. Pre-ingestion urine volume and osmolality were the same for both groups (p&gt;0.05). Following ingestion of the drink, urine volume amounted to 420±258 ml for those with the I allele and 337±160 ml for those without the I allele (p=0.293). This pilot study found that, although not statistically significant, participants with the I allele had a lower urine volume than those without the I allele, amounting to a reduction of approximately 80 ml (19%). Further investigations are now warranted in a larger participant cohort to determine whether the ACE genotype is statistically associated with fluid retention after ingestion.
The bacteriology of skin cysts*
Authors: BARBARA J. LEPPARD, JAMES P.S. THOMSON, W.C. NOBLE
Journal: British Journal of Dermatology (1977)

Thirty-nine clinically uninflamed cysts of the three most common varieties, epidermoid cysts, trichilemmal cysts and steatocystoma multiplex were removed under sterile conditions and the contents cultured under aerobic and anaerobic conditions. Seventy-three percent of epidermoid cysts grew significant numbers of organisms whereas none of the trichilemmal cysts did so. The organisms found were the common skin commensals, Staphylococcus epidermidis biotype I, anaerobic Gram positive cocci of the Gaffya type and Corynebacterium acnes Type I. This indicates that cysts which clinically have a punctum, namely epidermoid cysts, may become colonized with surface bacteria under normal circumstances. If they become colonized with pathogenic bacteria they become inflamed, a not uncommon clinical picture. The results from patients with steatocystoma multiplex were difficult to interpret because of the small numbers of patients involved and the unusual clinical appearance they presented.
508 EPIGENETIC METHYLATIONS AND EXPRESSIONS OF CASPASE 8 AND SURVIVIN IN HEPATOCELLULAR CARCINOMA
Authors: S. Cho, S.B. Cho, W.S. Lee, Y.E. Joo, H.S. Kim, J.H. Lee, J.S. Rew, S.K. Choi
Journal: Journal of Hepatology (2009)
New Paths of Investigation for Diagnosis and Therapy
Authors: E. Freund
Journal: Annals of the Rheumatic Diseases (1943)

THE RHEUMATIC DISEASES that these pains, which tend to arise in a less acute manner, are also generally of the focal type.5. The final suggestion is therefore that the myalgic spots which it is now widely agreed form the basis of many of the rheumatic syndromes of later life are sometimes a legacy from acute infections in earlier life.It is important to remember that the victims are often unaware of their presence until they become activated by factors such as chill, trauma, or focal sepsis.These factors should therefore be regarded as secondary and no longer causative in such cases.
Mechanisms of ventricular parasystole.
Authors: S Kinoshita
Journal: Circulation (1978)

Eight cases of ventricular parasystole are reported. In all these cases, regardless of whether parasystole seems intermittent or "continuous," the presence of second degree entrance block of the Mobitz type I was suggested. Parasystole alternated with concealed extrasystolic bigeminy showing occasional reentrant extrasystoles. Such intermittent parasystole appears to originate in the reentrant path of extrasystoles. Reentrant extrasystolic bigeminy was seen in a comparatively rapid sinus rhythm, whereas parasystolic bigeminy was seen in a comparatively slow sinus rhythm. The difference between the interectopic intervals during parasystolic bigeminy and during (manifest or concealed) extrasystolic bigeminy was comparatively small so that occasionally the difference was not distinct; on such an occasion the case showed a seemingly "continuous" parasystole. These observations strongly suggest the possibility that most cases of parasystole, whether intermittent or "continuous," may be governed by incomplete entrance block of the second degree.