tbuckley/cabot-search huggingface dataset:
Delayed Rectifier Channels in Human Ventricular Myocytes
Authors: Marieke W. Veldkamp, Antoni C.G. van Ginneken, Tobias Opthof, Lennart N. Bouman
Journal: Circulation (1995)

Previous studies have shown that in heart there are two kinetically distinct components of delayed rectifier current: a rapidly activating component (IKr) and a more slowly activating component (IKs). The presence of IKr and/or IKs appears to be species dependent. We studied the nature of the delayed rectifier current in human ventricle in whole-cell and single-channel experiments.Ventricular myocytes were obtained from hearts of patients with ischemic or dilated cardiomyopathy. Single-channel currents and whole-cell tail currents were recorded at negative potentials directly after return from a depolarizing step. Single-channel currents were measured in the cell-attached patch configuration with 140 mmol/L K+ in the pipette. In the present study, we identified a voltage-dependent channel with a single-channel conductance of 12.9 +/- 0.8 pS (mean +/- SEM, n = 5) and a reversal potential near to the K+ equilibrium potential, suggesting that the channel is selective to K+ ions. Channel activity was observed only after a depolarizing step and increased with the duration and amplitude of the depolarization, indicating time- and voltage-dependent activation. Activation at +30 mV was complete within 300 milliseconds, and the time constant of activation, determined in the whole-cell configuration, was 101 +/- 25 milliseconds (mean +/- SEM, n = 4). The voltage dependence of activation could be described by a Boltzmann equation with a half-activation potential of -29.9 mV and a slope factor of 9.5 mV. The addition of the class III antiarrhythmic drug E-4031 completely blocked channel activity in one patch. No indications for the presence of IKs were found in these experiments.The conformity between the properties of IKr and those of the K+ channel in the present study strongly suggests that IKr is present in human ventricle.
Recovery of Endothelial Cell Prostacyclin Production after Inhibition by Low Doses of Aspirin
Authors: Eric A. Jaffe, Babette B. Weksler
Journal: Journal of Clinical Investigation (1979)

Endothelial cells synthesize prostacyclin (PGI2), an unstable prostaglandin that inhibits platelet aggregation and serotonin release. Because cyclooxygenase, which is necessary for synthesis of PGI2, is inactivated by aspirin, we examined the effect of aspirin on PGI2 production by cultured human endothelial cells. Endothelial cells synthesize PGI2 (20.1±7.2 ng/106 cells, mean±SD) when stimulated with 20 μM sodium arachidonate for 2 min. PGI2 production is inhibited by low-dose aspirin (5 μM); the t½ of inactivation is 6.0±1.3 min (mean±SEM, n = 3). Thus, endothelial cell cyclooxygenase is as sensitive to aspirin as the enzyme in platelets. After 1 h incubation with aspirin, endothelial cell PGI2 production was inhibited 50% by 2.1±0.4 μM aspirin and was inhibited 90% by 6.2±0.9 μM aspirin (mean±SEM, n = 4). When endothelial cells were incubated with 100 μM aspirin, washed, and recultured, their ability to synthesize PGI2 returned to control levels in 35.6±1.0 h (mean±SEM, n = 4). Recovery of endothelial PGI2 production after aspirin depended on de novo protein synthesis because treatment with cycloheximide (3 μg/ml) inhibited recovery by 92%.
Quantitation of regional cardiac function by two-dimensional echocardiography. I. Patterns of contraction in the normal left ventricle.
Authors: R V Haendchen, H L Wyatt, G Maurer, W Zwehl, M Bear, S Meerbaum, E Corday
Journal: Circulation (1983)

Regional differences in wall motion and wall thickening were quantitated in the normal left ventricle using two-dimensional echocardiography (2-D echo). Using a computer-aided system, the left ventricle was subdivided in a standardized manner into 40 segments of five 2-D echo short-axis cross sections from the mitral valve level to the low left ventricle or apex. Measurements of sectional and segmental cavity areas, muscle areas and endocardial as well as epicardial perimeters, allowed assessment of contractile function using such indexes as endocardial systolic fractional area change (FAC), wall thickening (WTh), and circumferential fiber shortening (shortening). In 50 normal anesthetized, closed-chest dogs (including 10 studies in the conscious state) and in 32 normal humans, left ventricular contractile function increased significantly from base to apex. Thus, in anesthetized dogs, sectional FAC, WTh and shortening increased from left ventricular base to apex as follows: 39.4 +/- 5.1% to 61.6 +/- 7.2%, 20.5 +/- 6.6% to 46.7 +/- 11.5% and 22.7 +/- 3.4% to 35.4 +/- 5.9%, respectively. Similar trends were noted in conscious dogs. In man, sectional FAC, WTh and shortening also increased from the mitral valve to the low left ventricular level: 38.8 +/- 3.3% to 60.7 +/- 4.5%, 23.9 +/- 5.6% to 28.9 +/- 7.6% and 21.4 +/- 5.0% to 30.6 +/- 5.6%, respectively. Detailed segmental analysis in individual cross sections also revealed regional differences in contraction. Generally, contraction was most vigorous in posterior regions of the left ventricle. The septal regions exhibited lowest contraction at the base, but also the greatest increase from base to apex, both in the canine and human. Lateral regions did not show significant changes along the length of the left ventricle. Diastolic wall thickness also varied. We conclude that contraction in the normal left ventricle cannot be assumed to be uniform or symmetrical. These normal regional differences in function should be taken into account when evaluating altered physiologic states and in studying effects of therapeutic interventions.
TCT-484 Immunologic Basis of Clopidogrel Hypersensitivity and Diagnosis by Lymphocyte Toxicity Assay
Authors: Asim Cheema, Kushal J. Dighe, Henry R. Jakubovic, Radu M. Nanau, Ivana Kandic, Laura R. Finken
Journal: Journal of the American College of Cardiology (2014)
American Surgical Association Desires Articles on Fractures
Authors: John B. Roberts
Journal: JAMA (1913)

<h3>To the Editor:</h3> —The Committee on Fractures of the American Surgical Association desires to have two reprints of any paper dealing with the non-operative or operative treatment of open or compound fractures or the medicolegal relations of roentgenography to the diagnosis and treatment of fractures which has been published within the last five years. If authors have no reprints, the committee would be pleased to receive memoranda of the places of publication.
The Activated Stroma Index Is a Novel and Independent Prognostic Marker in Pancreatic Ductal Adenocarcinoma
Authors: Mert Erkan, Christoph W. Michalski, Simon Rieder, Carolin Reiser–Erkan, Ivane Abiatari, Armin Kolb, Nathalia A. Giese, Irene Esposito, Helmut Friess, Jörg Kleeff
Journal: Clinical Gastroenterology and Hepatology (2008)
Polyarteritis and Intrarenal Renal Artery Aneurysms
Authors: Leslie Dornfeld
Journal: JAMA (1971)

The diagnosis of polyarteritis nodosa is frequently difficult. Renal biopsy is often hazardous due to severe hypertension or azotemia. Renal arteriographic changes described herein are considered specific for polyarteritis nodosa. These include intrarenal renal artery aneurysms confined to the distal segmental, interlobar, and occasionally the arcuate arteries.
133. Comparison of Adeno-Associated Viral Vector Serotypes 1, 2, 5 and 6 for Gene Transfer to Neurons and Glia In Vitro
Authors: Doug B. Howard, Juan Li, Yun Wang, Brandon K. Harvey
Journal: Molecular Therapy (2005)

One prominently used viral vector, adeno-associated virus (AAV), successfully transduces cells of the central nervous system in vivo. Studies have compared the in vivo transduction efficiency of several AAV serotypes, primarily 1, 2 and 5. Here, we compare the transduction of cells in primary neuronal cultures derived from rat embryonic brain tissue using the AAV serotypes 1, 2, 5 and 6, purified by CsCI centrifugation. A self-complementing AAV vector containing AAV2 terminal repeats flanking a green fluorescent protein expressed from the CMV promoter was packaged using the cap gene from AAV serotypes 1, 2, 5 or 6. Cultures were treated on DIV6 then examined by immunofluorescence 7 days later. Compared to untreated cultures, all four serotypes did not alter cell viability as measured by cell permeability assays (LDH release and nuclear dye exclusion). At comparable MOIs, serotypes 1 and 6 yielded a higher number of GFP+ cells than types 2 and 5. At an MOI of 20,000, serotypes 1, 2 and 6 expressed GFP in NeuN+ or GFAP+ cells with similar efficiency. At the same MOI, serotype 5 expressed GFP in more GFAP+ than NeuN+ cells. However, with all four serotypes tested, approximately 10|[ndash]|20% of the GFP+ cells were negative for both GFAP and NeuN. These results demonstrate that AAV serotypes 1, 2, 5 and 6 can transduce both neurons and astrocytes in vitro with minimal toxicity and exhibit a similar transduction profile to that observed in vivo.
Book Review Manual of Clinical Microbiology Fifth edition. Edited by Albert Balows, with William J. Hausler, Jr., Kenneth L. Herrmann, Henry D. Isenberg, and H. Jean Shadomy. 1364 pp., illustrated. Washington, D.C., American Society for Microbiology, 1991. $84. ISBN 1–55581–029–2 .
Authors: David R. Snydman
Journal: New England Journal of Medicine (1992)
Neonatal Jaundice in Glucose - 6 - Phosphate - Dehydrogenase - Deficient Infants
Authors: Ph. Fessas, S. A. Doxiadis, T. Valaes
Journal: BMJ (1962)

The findings are reported of a small survey of the attitudes of mothers using local authority infant and child health and welfare services in three widely contrasted areas of England and Wales.The results are compared and contrasted with those of the National Child Health Survey and the Newcastle-on-Tyne 1,000 Family Study.Special reference is made to the fact that over 60 % consultations are made with the health visitor-a circumstance the mothers seem readily to accept; and to the fact that her services are most popular with mothers of all ages who have a young first-born infant.Some conclusions are drawn on the possible effects these findings may have on the shape of infant and child preventive care that general practitioners are developing within their own practices ; in particular it is pointed out that such ventures are unlikely to be successful unless the assistance of a health visitor is obtained.In addition to the Study Group, whose names are listed above, many people gave invaluable assistance in this survey.
P1082 TO COMPARE THE EFFICACY OF URSO-DEOXYCHOLIC ACID, ONDANSETRON AND NALTREXONE IN THE SEVERE PRURITUS OF ACUTE CHOLESTATIC VIRAL HEPATITIS; A RANDOMIZED TRIAL
Authors: A.K. Jain, C. Waghmare, S. Adkar, S. Sircar, M. Jain, S.P. Jaiswal
Journal: Journal of Hepatology (2014)
Referral from Delores
Authors: Jack Coulehan
Journal: Annals of Internal Medicine (2004)

Ad Libitum16 November 2004Referral from DeloresJack Coulehan, MDJack Coulehan, MDFrom Setauket, NY 11733.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-141-10-200411160-00011 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Aside from the fleshy mole on her chin,her face is featureless—which isn't truebut seems so, so late in the afternoonwith a storm about to come through.She doesn't have a complaint, but rathera note from Delores Hernandez,her aide at the home, who says the odorsickens the residents and causes usto wonder if she suffers from gangreneor something. Maybe it's intestinal gasshe leaks, it's so persistent. Anythingwe try is futile. Mary was homeless.We had to delouse her when she cameat Thanksgiving, but now she's continentof urine, except at night. We give her cleanundergarments in the morning. We senther ... Author, Article, and Disclosure InformationAffiliations: From Setauket, NY 11733.Current Author Address: Jack Coulehan, MD, 4 Townsend Court, Setauket, NY 11733. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 16 November 2004Volume 141, Issue 10Page: 800KeywordsSmellStormsUrine ePublished: 16 November 2004 Issue Published: 16 November 2004 Copyright & PermissionsCopyright © 2004 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
IMPACT OF PACLITAXEL-ELUTING STENTS IN DIABETIC PATIENTS WITH ACUTE MYOCARDIAL INFARCTION UNDERGOING PRIMARY ANGIOPLASTY: ANALYSIS FROM THE HORIZONS AMI TRIAL
Authors: Bernhard Witzenbichler, Jochen Woehrle, Giulio Guagliumi, Jan Peruga, Bruce Brodie, Dariusz Dudek, Ran Kornowski, Franz Hartmann, Helen Parise, Alexandra Lansky, Eugenia Nikolsky, Roxana Mehran, Gregg Stone
Journal: Journal of the American College of Cardiology (2010)
Plasma Adiponectin, Body Mass Index, and Mortality in Patients With Chronic Heart Failure
Authors: Caroline Kistorp, Jens Faber, Søren Galatius, Finn Gustafsson, Jan Frystyk, Allan Flyvbjerg, Per Hildebrandt
Journal: Circulation (2005)

Recent studies have suggested that higher body mass index (BMI) is associated with improved prognosis in chronic heart failure (CHF). The adipocytokine adiponectin is inversely associated with BMI, and in healthy subjects, low adiponectin is a predictor of mortality. In a prospective study, we therefore evaluated the association between plasma adiponectin levels and mortality among patients with CHF.In 195 CHF patients (age 69.3+/-10.2 years, BMI 27.3+/-5.2 kg/m2, left ventricular ejection fraction 30+/-8.9%, mean+/-SD), plasma adiponectin and N-terminal pro brain natriuretic peptide (NT-proBNP) were measured at baseline. Adiponectin was positively associated with NT-proBNP (beta=0.47, P<0.001), and both biomarkers were negatively associated with BMI (beta=-0.43, P<0.001 for adiponectin and beta=-0.38, P<0.001 for NT-proBNP, respectively) During a median follow-up of 2.6 years, 46 (23.5%) of the patients died. After adjustment for clinical variables associated with CHF severity (age, systolic blood pressure, left ventricular ejection fraction <25%, duration of CHF, and creatinine clearance) and for NT-proBNP, the hazard ratio of mortality for values in the 2 upper tertiles relative to the lowest tertile of adiponectin was 3.23 (P=0.032). BMI predicted mortality independently of clinical parameters of CHF severity (hazard ratio=0.63, P=0.012), but this association became insignificant after additional adjustment for NT-proBNP (hazard ratio=0.74, P=0.13).A high adiponectin level was a predictor of mortality, independent of risk markers of CHF severity, presumably because of its role as a marker for wasting. BMI was also associated with mortality, but a part of this relation may be mediated by adiponectin and NT-proBNP levels.
Anaphylaxis to 5-methoxypsoralen during photochemotherapy.
Authors: F.J. Legat, P. Wolf, B. Kranke
Journal: British Journal of Dermatology (2001)

Journal Article Anaphylaxis to 5‐methoxypsoralen during photochemotherapy Get access F.J. Legat, F.J. Legat Department of Dermatology, Karl‐Franzens‐University Graz, Auenbruggerplatz 8, A‐8036 Graz, Austria Dr F.J.Legat. E‐mail: franz.legat@kfunigraz.ac.at Search for other works by this author on: Oxford Academic Google Scholar P. Wolf, P. Wolf Department of Dermatology, Karl‐Franzens‐University Graz, Auenbruggerplatz 8, A‐8036 Graz, Austria Search for other works by this author on: Oxford Academic Google Scholar B. Kränke B. Kränke Department of Dermatology, Karl‐Franzens‐University Graz, Auenbruggerplatz 8, A‐8036 Graz, Austria Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 145, Issue 5, 1 November 2001, Pages 821–822, https://doi.org/10.1046/j.1365-2133.2001.04464.x Published: 01 November 2001
Injectable and thermo-sensitive PEG-PCL-PEG copolymer/collagen/n-HA hydrogel composite for guided bone regeneration
Authors: ShaoZhi Fu, PeiYan Ni, BeiYu Wang, BingYang Chu, Lan Zheng, Feng Luo, JingCong Luo, ZhiYong Qian
Journal: Biomaterials (2012)
[556] PREVALENCE OF EACH FIBROSIS STAGE IS A MAJOR FACTOR OF VARIABILITY WHEN ASSESSING THE AREA UNDER THE ROC CURVES IN THE EVALUATION OF LIVER FIBROSIS MARKERS
Authors: T. Povnard, P. Halfon, L. Castera, M. Munteanu, F. Imbert Bismut, V. Ratziu, M. Bourliere, V. de Ledinghen, G. Fibropaca
Journal: Journal of Hepatology (2007)
Effect of histamine on cardiac sarcolemmal calcium binding as indicated by use of ionic lanthanum
Authors: S. A BARMAN, M. D OLSON, J. T SAARI
Journal: Cardiovascular Research (1987)

To determine whether the inotropic effect of histamine might be associated with enhanced calcium binding to the sarcolemmal-glycocalyx complex, the effect of histamine on lanthanum binding to the cell membrane in the presence of verapamil was assessed. Rabbit hearts were perfused firstly with histamine, secondly with histamine and verapamil, and subsequently with histamine in the presence of lanthanum and verapamil. Subsequent transmission electron microscopy showed the presence of bound lanthanum on both the cellular surface and cytoplasmic vesicles of most cardiac myocytes, which is in contrast to the absence of lanthanum binding that occurs in the presence of verapamil alone. Furthermore, this histamine mediated enhancement of lanthanum binding is not affected by the H1 antagonist diphenhydramine but is reduced by the H2 antagonist cimetidine. This visual evidence of an apparent enhancement of lanthanum binding under histamine and subsequent attenuation with cimetidine supports the hypothesis that histamine may exert its positive inotropic effect by causing increased calcium binding to the sarcolemmal-glycocalyx complex of cardiac muscle and that the effect is mediated at least in part by H2 receptors.
Spironolactone Diuresis in Patients with Cirrhosis and Ascites
Authors: R. C. Eggert
Journal: BMJ (1970)

Increased aldosterone levels with consequent or diuretic-potentiated electrolyte abnormalities are an important consideration when patients with cirrhosis and ascites undergo diuresis. A simple clinical method using the urinary Na/K ratio as a guide to spironolactone dosage is outlined. Patients with a ratio greater than 1 responded well to 100 mg. of spironolactone a day; those when it was one or less responded well to 200 to 1,000 mg. a day. Administration of spironolactone alone (11 patients) or as the main diuretic (three patients) was a safe and effective means of inducing sustained uncomplicated diuresis in all these patients.
Idelalisib — A PI3Kδ Inhibitor for B-Cell Cancers
Authors: David A. Fruman, Lewis C. Cantley
Journal: New England Journal of Medicine (2014)

The phosphoinositide 3-kinase (PI3K) signal transduction pathway is highly active in tumors and drives many of the hallmarks of cancer. Efforts to develop PI3K inhibitors for oncology have been complicated by the existence of four distinct PI3K catalytic isoforms with partially overlapping functions. In addition, diverse resistance mechanisms and on-target toxic effects have been observed with many PI3K inhibitors.1,2 In this issue of the Journal, two clinical teams report that idelalisib, a selective inhibitor of the delta isoform of PI3K, provides impressive efficacy and has an acceptable side-effect profile when used to treat patients with certain B-cell cancers.3, . . .
p53 Independent epigenetic-differentiation treatment in xenotransplant models of acute myeloid leukemia
Authors: K P Ng, Q Ebrahem, S Negrotto, R Z Mahfouz, K A Link, Z Hu, X Gu, A Advani, M Kalaycio, R Sobecks, M Sekeres, E Copelan, T Radivoyevitch, J Maciejewski, J C Mulloy, Y Saunthararajah
Journal: Leukemia (2011)
THE RECOGNITION AND PROMPT REMOVAL OF POSTNASAL ADENOIDS IN CHILDREN.
Authors: LOUIS J. LAUTENBACH
Journal: JAMA (1903)

The importance of the consideration of our subject is evident when we observe the divergent views presented by different authors as to the instruments to be used in operations and the varying ideas as to anesthesia. That the subject is still uppermost in the mind of the specialist is evident from a consideration of this year's programs of the Section on Laryngology and Otology, where three papers out of a total of thirty-three are presented on the growths in the nasopharynx, and of this Section on Diseases of Children, in which three papers out of a total of thirty-nine are devoted to the same subject. <h3>FREQUENCY AND CAUSES.</h3> As to its frequency, opinions of authors are much at variance. Ingals of Chicago reports but 2 per cent. out of ten thousand private chest, throat and nose patients, while Frankenberg reports 59 per cent. in the 158 inmates of
A survey of hospital toilet facilities.
Authors: A. F. Travers, E. Burns, N. D. Penn, S. C. Mitchell, G. P. Mulley
Journal: BMJ (1992)

To assess the quality of toilet facilities available for disabled people in a large provincial teaching hospital.Survey of toilet facilities for patients on the wards and in the outpatient department.Teaching hospital in Leeds.Although the quality of toilet facilities varied, none met the standards recommended by the British Standards Institution. The worst facilities were found on a ward accommodating elderly patients, where the toilets were unsuitable for use by disabled people and bedside commodes had to be used instead.Toilet provision within a major hospital failed to meet standards required for disabled people. Admission to hospital may therefore result in loss of independence and dignity. If hospitals are to be centres of excellence, greater consideration must be given to the requirements of disabled people in the design of new wards, and current inadequate facilities should be upgraded.
Time-Related Changes in Left Ventricular Function After Double Valve Replacement for Combined Aortic and Mitral Regurgitation in a Young Rheumatic Population
Authors: Daniel Skudicky, Mohammed R. Essop, Pinhas Sareli
Journal: Circulation (1997)

Background The long-term effects of double valve replacement on left ventricular function in patients with combined severe rheumatic aortic and mitral regurgitation have not been reported previously. Furthermore, the importance of chordal preservation in this group of patients is unknown. Methods and Results Serial clinical and echocardiographic evaluations were performed prospectively in 44 patients who underwent double valve replacement for combined aortic and mitral regurgitation. Chordae to the posterior mitral leaflet were preserved in 27 patients. Mean follow-up was 40±19 months. Left ventricular end-diastolic diameter decreased significantly 3 months after surgery (from 66±10 to 52±11 mm; P &lt;.001) without a substantial change in end-systolic diameter, resulting in a significant decline in ejection fraction (from 60±9% to 48±15%; P &lt;.001). At 1 year, a significant reduction in end-systolic dimension was observed without a concomitant decline in end-diastolic diameter, thus normalizing the ejection fraction (55±12%; P =.17 versus baseline). No further changes were seen at latest follow-up. Multivariate regression analysis identified baseline end-systolic diameter and ejection fraction as independent predictors of postoperative systolic performance. Chordal preservation did not emerge as a univariate or multivariate predictor. Conclusions After an initial postoperative decline in ejection fraction, normalization in left ventricular systolic function may be expected 1 year after double valve replacement for combined rheumatic mitral and aortic regurgitation. End-systolic diameter and ejection fraction are the only independent predictors of postoperative left ventricular performance.