tbuckley/cabot-search huggingface dataset:
Robust and Efficient Regulation of Transgene Expression in Vivo by Improved Tetracycline-Dependent Lentiviral Vectors
Authors: Elisa Vigna, Simona Cavalieri, Laurie Ailles, Massimo Geuna, Rainer Loew, Hermann Bujard, Luigi Naldini
Journal: Molecular Therapy (2002)

We developed a panel of lentiviral vectors that displayed tetracycline-regulated transgene expression over two orders of magnitude in bulk, non-selected populations of transduced cells in vitro and in vivo. The robust expression and homogeneous response indicated that most transduced vector genomes were transcription competent and responsive to regulation, providing the lentiviral vector with a novel competitive advantage for gene transfer. After ex vivo transduction and transplantation of cord blood CD34+ cells into NOD/SCID mice, reporter gene expression could be switched "on" and "off" in human hematopoietic cells in vivo for prolonged times, proving integration of the regulated expression system into long-term repopulating cells. By vector injection into established tumor grafts, we achieved efficient delivery and quantitative regulation of transgene expression in vivo. By these approaches, gene function studies can now be performed in in vivo models of human hematopoiesis and cancer. In the future, regulated lentiviral vectors will improve the safety and efficacy of gene therapy.
Behçet’s Disease and Psychosomatic Patterns of Thinking
Authors: Franco Orsucci
Journal: Psychotherapy and Psychosomatics (1996)

<i>Background:</i> A single case of Behçet's disease was studied throughout several consultations during a period of 15 years. <i>Methods:</i> The evolution of the disease was related to important events in the subject's life, cognitive patterns, psychopathological features and, particularly, his relationships to women and to his family. <i>Results:</i> Some interesting complex correlations between illness course and psychosocial events and states were highlighted. <i>Conclusions:</i> Future prospective studies could better elucidate this multiple feedback.
Submucosal tumour in a young girl
Authors: N. Hayashi, K. Mima, Y. Nagai, Y. Baba, Y. Imamura, Y. Hiyoshi, T. Nakamura, N. Yoshida, E. Toyama, N. Miyanari, M. Watanabe, H. Baba
Journal: Gut (2009)

A 15-year-old girl was referred to our hospital for further investigation because of an abnormal shadow detected on chest x ray (fig 1A) obtained for her annual physical examination at high school.



Figure 1 
(A) Chest x ray reveals an abnormal shadow in the stomach. (B) Upper gastrointestinal endoscopy shows …
Unpalatable results force cleft surgeons to rethink
Authors: H. Bower
Journal: BMJ (1998)

A damning report on cleft lip and palate repair has forced British surgeons to confront a longstanding disagreement over which specialty should carry out the technically challenging surgery.

After investigating 297 children aged 5 and 277 aged 12 who had all undergone cleft repair in Britain, the Clinical Standards Advisory Group found that two out of five had poor dental bite, less than a third had a good lip appearance at age 12, and under half could speak with normal intelligibility at that age. The group's committee said that of the 57 units currently carrying out cleft palate repair, only six …
Nazi origins of an anatomy text: the Pernkopf atlas
Authors: H. A. Israel
Journal: JAMA (1996)

To the Editor.  —The abuses of medicine perpetrated during the Hitler regime pervaded the entire medical profession of the Third Reich including the academic elite. One legacy of that tragic era endures today through the continued publication of a critically acclaimed atlas, Pernkopf Anatomy , 1 named after the anatomist Professor Eduard Pernkopf of the University of Vienna. The original Pernkopf work contains more than 800 detailed paintings of dissections. 2 A leading Nazi, Pernkopf became dean of the University of Vienna Faculty of Medicine after Germany annexed Austria in 1938. 3 From that position he led the purge against the Jewish faculty in Vienna. 4 Pernkopf s artists expressed their political sympathies by incorporating Nazi icons (such as the letters SS or a swastika) as part of their signatures in the original volume. Recent editions include paintings from the earlier volumes with most of the Nazi icons eliminated.  The precise
Myocardial Metabolism Studied in the Isolated Perfused Rat Heart with Radioisotopes: Importance of Free Fatty Acids.
Authors: J. C. Shipp
Journal: Annals of Internal Medicine (1963)

s1 April 1963Myocardial Metabolism Studied in the Isolated Perfused Rat Heart with Radioisotopes: Importance of Free Fatty Acids.J. C. Shipp, M.D., L. H. Opie, M.D., O. E. Matos, B.S., L. Crevasse Jr., M.D. (Associate)J. C. Shipp, M.D.Search for more papers by this author, L. H. Opie, M.D.Search for more papers by this author, O. E. Matos, B.S.Search for more papers by this author, L. Crevasse Jr., M.D. (Associate)Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-58-4-716_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThis approach permits a direct study of the uptake and cellular fat of a particular substrate. The uptake of free fatty acid and glucose, lactate production, residual and labeled heart glycogen, and the amount of label appearing in CO2and in tissue fatty acids were measured. When present alone, the uptake of glucose-u-C14and of palmitate-1-C14was a linear function of concentration over the physiologic range. Each was promptly oxidized to CO2. Glucose was incorporated into glycogen, and a significant fraction appeared as lactate. Palmitate was also present as tissue fatty acids; when combined, fatty acid was oxidized in preference... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Gainesville, Fla. (BS) PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 April 1963Volume 58, Issue 4Page: 716-716KeywordsAttentionFatsFatty acidsGlucoseGlycogensHeartLactates Issue Published: 1 April 1963 PDF downloadLoading ...
Abstract 4662: Genetic predictors of clinical outcomes for patients with non-small cell lung cancer
Authors: Xia Pu, Margaret R. Spitz, Chris Amos, Yuanqing Ye, Michelle A. Hildebrandt, David Chang, Charles Lu, David Stewart, Jack A. Roth, Scott M. Lippman, David Christiani, Xifeng Wu
Journal: Cancer Research (2011)

Abstract Background: Emerging evidence has shown that genetic factors can interact with host factors to influence patient's clinical outcomes. Inflammatory biomarkers have been identified as prognostic factors independent of tumor stage. To test the hypothesis that genetic variants in inflammation-related genes may modulate clinical outcome in non-small cell lung cancer (NSCLC) patients, we conducted a three-stage study, including an initial discovery phase and two validation phases. Methods: In the discovery phase, we analyzed the association between 19,458 SNPs in inflammation-related genes and SNPs from GWAS risk analysis, and clinical outcomes in 1,408 NSCLC patients. In the validation phases, the significant SNPs (p&amp;lt;0.05) were first validated in an additional 1,104 NSCLC patients from MD Anderson and then validated in 978 NSCLC patients from Harvard University. Meta-analysis was used to summarize the combined effect of each SNP in all three stages. Results: A total of 16 SNPs were identified as top candidate predictors of respective outcomes after showing consistent associations in all three stages. Among them, 5 SNPs were associated with overall survival in the entire populations. The most significant SNP is located on chromosome 6 (discovery: HR=1.49, 95%CI=1.10-2.01, P=0.010; combined validation: HR=1.45, 95%CI=1.17-1.78, P=0.001; all combined: HR=1.46, 95% CI=1.23-1.73, P=1.59×10-5). Five SNPs were associated with overall survival in late stage unresectable patients receiving chemotherapy. The most significant SNP, located on chromosome 13, consistently showed increased risk of death in all three phases (discovery: HR=1.40, 95%CI=1.09-1.81, P=0.010; combined validation: HR=1.36, 95%CI=1.14-1.63, P=0.001; all combined: HR=1.37, 95% CI=1.19-1.59, P=2.26×10-5). Six SNPs were consistently associated with progression in early stage patient receiving surgery only. The top SNP was on chromosome 1 with protective effect against developing progression in all three phases (discovery: HR=0.52, 95%CI=0.32-0.86, P=0.033; combined validation: HR=0.63, 95%CI=0.49-0.82, P=0.001; all combined: HR=0.62, 95% CI=0.5-0.79, P=5.47×10-5). To our knowledge, this is the first study to systematically evaluate the association of genetic variations in major inflammation genes and NSCLC patients’ clinical outcomes with a multi-stage design using two of the largest lung studies in the US. Our study provides evidence that genetic variations in inflammation genes may affect clinical outcomes in NSCLC patients. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4662. doi:10.1158/1538-7445.AM2011-4662
Mandating Vaccination: What Counts as a “Mandate” in Public Health and When Should They Be Used?
Authors: Matthew K. Wynia
Journal: The American Journal of Bioethics (2007)

Recent arguments over whether certain public health interventions should be mandatory raise questions about what counts as a "mandate." A mandate is not the same as a mere recommendation or the standard of practice. At minimum, a mandate should require an active opt-out and there should be some penalty for refusing to abide by it. Over-loose use of the term "mandate" and the easing of opt-out provisions could eventually pose a risk to the gains that truly mandatory public health interventions, such as childhood vaccines, have provided over the last 50 years. Already, confusion about what counts as a mandate, and about what criteria should be used to determine when a public health intervention should be implemented as a mandate, has led to some inappropriate public policy decisions. For instance, by any reasonable criteria, the yearly influenza vaccine should be mandatory for health care workers. To enforce this mandate, those who refuse vaccination should be required to sign a waiver, and patients—especially those at high risk from flu—should be informed when they receive care from unvaccinated practitioners.
Interleukin-10 controls neutrophilic infiltration, hepatocyte proliferation, and liver fibrosis induced by carbon tetrachloride in mice
Authors: Hubert Louis, Jean-Luc Van Laethem, Wei Wu, Eric Quertinmont, Chantal Degraef, Kit Van den Berg, Anne Demols, Michel Goldman, Olivier Le Moine, Albert Geerts, Jacques Devière
Journal: Hepatology (1998)

The role of the anti–inflammatory cytokine interleukin–10 (IL–10) was investigated in the mouse model of liver injury induced by carbon tetrachloride (CCl 4 ). To address the role of endogenous IL–10 production, acute hepatitis was induced by CCl 4 in C57Bl/6 IL–10 gene knock out (KO) and wild–type (WT) mice. After CCl 4 challenge, serum and liver levels of tumor necrosis factor–alpha (TNF–α) and serum levels of transforming growth factor–beta 1 (TGF–β1) increased and were significantly higher in IL–10 KO mice, whereas IL–6 serum levels were only slightly increased compared with WT mice. At histological examination, the livers disclosed a significantly more prominent neutrophilic infiltration in IL–10 KO mice 12 and 24 hours after CCl 4 injection. In contrast, hepatocyte necrosis, evaluated by histological examination and serum alanine aminotransferase levels, was only marginally affected. The proliferative response of hepatocytes, assessed by the proliferating cell nuclear–antigen labeling index, was significantly increased in IL–10 KO mice, compared with WT mice 48 hours after CCl 4 injection. Finally, repeated CCl 4 injections led to more liver fibrosis in IL–10 KO mice after 7 weeks. In conclusion, endogenous IL–10 marginally affects the hepatocyte necrosis although it controls the acute inflammatory burst induced by CCl 4 . During liver repair, it limits the proliferative response of hepatocytes and the development of fibrosis.
Interleukin-2-induced survival of natural killer (NK) cells involving phosphatidylinositol-3 kinase-dependent reduction of ceramide through acid sphingomyelinase, sphingomyelin synthase, and glucosylceramide synthase
Authors: Yoshimitsu Taguchi, Tadakazu Kondo, Mitsumasa Watanabe, Michihiko Miyaji, Hisanori Umehara, Yasunori Kozutsumi, Toshiro Okazaki
Journal: Blood (2004)
Thallium-201 myocardial tomography with intravenous infusion of adenosine triphosphate in diagnosis of coronary artery disease
Authors: Masao Miyagawa, Seishi Kumano, Michihito Sekiya, Kouki Watanabe, Hiroshi Akutzu, Tsuneo Imachi, Shuji Tanada, Ken Hamamoto
Journal: Journal of the American College of Cardiology (1995)
Evidence That Plasma Leptin and Insulin Levels are Associated With Body Adiposity Via Different Mechanisms
Authors: Michael W Schwartz, Ronald L Prigeon, Steven E Kahn, Marjery Nicolson, Jason Moore, Andrew Morawiecki, Edward J Boyko, Daniel Porte
Journal: Diabetes Care (1997)

OBJECTIVE Like insulin, the adipocyte hormone, leptin, circulates at levels proportionate to body adiposity. Because insulin may regulate leptin secretion, we sought to determine if plasma leptin levels are coupled to body adiposity via changes in circulating insulin levels or insulin sensitivity and whether leptin secretion from adipocytes is impaired in subjects with NIDDM. RESEARCH DESIGN AND METHODS We used multiple linear regression to analyze relationships between BMI (a measure of body adiposity) and fasting plasma levels of leptin and insulin in 98 nondiabetic human subjects (68 men/30 women) and 38 subjects with NIDDM (27 men/11 women). The insulin sensitivity index (S1) was also determined in a subset of nondiabetic subjects (n = 38). RESULTS Fasting plasma leptin concentrations were correlated to both BMI (r = 0.66, P = 0.0001) and fasting plasma insulin levels (r = 0.65, P = 0.0001) in nondiabetic men and women (r = 0.58, P = 0.0009 for BMI; r = 0.47, P = 0.01 for insulin). While the plasma leptin level was also inversely related to S1 (r = −0.35; P = 0.03), this association was dependent on BMI, whereas the association between insulin and S1 was not. Conversely, the relationship between plasma leptin and BMI was independent of S1, whereas that between insulin and BMI was dependent on S1. The relationship between plasma leptin levels and BMI did not differ significantly among NIDDM subjects from that observedin nondiabetic subjects. CONCLUSIONS We conclude that 1) body adiposity, sex, and the fasting insulin level are independently associated with plasma leptin level; 2) because NIDDM doesnot influence leptin levels, obesity associated with NIDDM is unlikely to result from impaired leptin secretion; and 3) insulin sensitivity contributes to the association between body adiposity and plasma levels of insulin, but not leptin. The mechanisms underlying the association between body adiposity and circulating levels of these two hormones, therefore, appear to bedifferent.
Benchmarking patient experiences in colonoscopy using the Global Rating Scale
Authors: J. Sint Nicolaas, V. de Jonge, I. Korfage, F. ter Borg, J. Brouwer, D. Cahen, W. Lesterhuis, R. Ouwendijk, E. Kuipers, M. van Leerdam
Journal: Endoscopy (2012)

The Global Rating Scale (GRS) is a quality assurance program that was developed in England to assess patient-centered care in endoscopy. The aim of the current study was to evaluate patient experiences of colonoscopy using the GRS in order to compare different departments and to provide benchmarks. The study also evaluated factors associated with patient satisfaction.A GRS questionnaire was used both before and after the procedure in outpatients undergoing colonoscopy. The questionnaire assessed the processes associated with the colonoscopy, from making the appointment up until discharge. Mean values and ranges of 12 endoscopy departments were calculated together with P values in order to assess heterogeneity.In total, 1904 pre-procedure and 1532 (80 %) post-procedure questionnaires were returned from 12 endoscopy departments. The mean time patients had to wait for their procedure was 4.3 weeks (range 3.1 - 5.8 weeks), and 54 % (range 35 - 64 %; P < 0.001) reported being given a choice of appointment dates/times. Discomfort during colonoscopy was reported by 20 % (range 8 - 40 %; P < 0.001). Recovery room privacy was satisfactory for 76 % of patients (range 66 - 90 %; P < 0.05). The majority of patients reported being sufficiently informed about what to do in case of problems after discharge (79 %, range 43 - 98 %; P < 0.001), and 85 % of individuals stated that they would be willing to repeat the colonoscopy procedure (range 72 - 92 %; P < 0.001). Factors associated with a decreased willingness to return were the burdensome bowel preparation (odds ratio [OR] = 0.25; P < 0.001), "rushing staff" attitude (OR = 0.57; P < 0.05), low acceptance of the procedure (OR = 0.42; P < 0.01), and more discomfort than expected (OR = 0.54; P < 0.05).Overall patient experiences with colonoscopy were satisfactory, but they also showed considerable variation. This study shows that use of a GRS patient questionnaire is feasible in the Dutch endoscopy setting for the assessment of patient experience. The significant variability between endoscopy units can be used to benchmark services and enable shortcomings to be identified.
Acyclovir and Ganciclovir in Cytomegalovirus
Authors: Gary B. Moss
Journal: Annals of Internal Medicine (1994)

Letters1 September 1994Acyclovir and Ganciclovir in CytomegalovirusGary B. Moss, MDGary B. Moss, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-121-5-199409010-00019 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:In their study, Singh and colleagues [1] propose “to assess the efficacy of high-dose oral acyclovir compared with preemptive, short-course ganciclovir therapy … to prevent CMV [cytomegalovirus] disease in live transplant patients.” Although their data strongly support the efficacy of ganciclovir over acyclovir in these patients, they do not directly compare these two medications.Each patient was randomly assigned to receive either high-dose oral acyclovir or no prophylaxis. If patients in either group later developed evidence of active CMV infection, they received a short course of gancyclovir to prevent progression of disease.Because all patients in both ...Reference1. Singh N, Yu VL, Mieles L, Wagener MM, Miner RC, Gayowski T. High-dose acyclovir compared with short-course preemptive ganciclovir therapy to prevent cytomegalorvirus disease in liver transplant recipients. A randomized trial. Ann Intern Med. 1994; 120:375-81. Google Scholar Author, Article, and Disclosure InformationAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 September 1994Volume 121, Issue 5Page: 385KeywordsCytomegalovirus infectionInfectious diseasesLiver transplantationProphylaxisRandomized trials Issue Published: 1 September 1994 CopyrightCopyright © 1994 by American College of Physicians. All Rights Reserved.PDF DownloadLoading ...
Cisplatin Dose Intensity in Non-Small Cell Lung Cancer: Phase II Results of a Day 1 and Day 8 High-Dose Regimen
Authors: D. R. Gandara, H. Wold, E. A. Perez, A. B. Deisseroth, J. Doroshow, F. Meyers, K. McWhirter, J. Hannigan, M. W. De Gregorio
Journal: JNCI Journal of the National Cancer Institute (1989)

Journal Article Cisplatin Dose Intensity in Non-Small Cell Lung Cancer: Phase II Results of a Day 1 and Day 8 High-Dose Regimen Get access David R. Gandara, David R. Gandara * Northern California Oncology GroupBlemont, CADivision of hematology/Oncology, University of California, Davis; and Veterans Administration Medical CenterMartinez, CA *Correspondence to: Dr. David R. Gabdara, University of California Service, VA Medical Center 612/111H, 150 Muir Rd., Martinez, CA 94553. Search for other works by this author on: Oxford Academic PubMed Google Scholar Howard Wold, Howard Wold Northern California Oncology GroupBlemont, CAHematology/Oncology Service, Letterman Army Medical CenterSan Francisco Search for other works by this author on: Oxford Academic PubMed Google Scholar Edith A. Perez, Edith A. Perez Northern California Oncology GroupBlemont, CADivision of hematology/Oncology, University of California, Davis; and Veterans Administration Medical CenterMartinez, CA Search for other works by this author on: Oxford Academic PubMed Google Scholar Albert B. Deisseroth, Albert B. Deisseroth Northern California Oncology GroupBlemont, CADivision of Hematology/Oncology, University of California, San Francisco; and Veterans Administration Medical CenterSan Francisco Search for other works by this author on: Oxford Academic PubMed Google Scholar J. Doroshow, J. Doroshow Northern California Oncology GroupBlemont, CACity of Hope National Medical CenterDuarte, CA Search for other works by this author on: Oxford Academic PubMed Google Scholar F. Meyers, F. Meyers Northern California Oncology GroupBlemont, CADivision of hematology/Oncology, University of California, Davis; and Veterans Administration Medical CenterMartinez, CA Search for other works by this author on: Oxford Academic PubMed Google Scholar K. McWhirter, K. McWhirter Northern California Oncology GroupBlemont, CA Search for other works by this author on: Oxford Academic PubMed Google Scholar J. Hannigan, J. Hannigan Northern California Oncology GroupBlemont, CA Search for other works by this author on: Oxford Academic PubMed Google Scholar Michael W. De Gregorio Michael W. De Gregorio Northern California Oncology GroupBlemont, CACancer Research Institute, University of CaliforniaSan Francisco, CA Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 81, Issue 10, 22 May 1989, Pages 790–794, https://doi.org/10.1093/jnci/81.10.790 Published: 22 May 1989 Article history Received: 22 December 1988 Revision received: 15 February 1989 Accepted: 16 February 1989 Published: 22 May 1989
More "cot deaths" occur in day care than at home
Authors: S. Gottlieb
Journal: BMJ (2000)

Many cases of the sudden infant death syndrome (cot deaths) occur in day care settings, where carers may be less aware of the importance of putting babies to sleep on their backs, new research has found.

In a study of 1916 cases of the sudden infant death syndrome in 11 US states, researchers found that about 20 —391 deaths—occurred in day care settings. The biggest risk seemed to be in home day …
Lipid Research Clinics Program
Authors: Evan M. Torch
Journal: JAMA (1984)

<h3>To the Editor.—</h3> The publication of the landmark study by the Lipid Research Clinics Program<sup>1</sup>listed numerous risk variables for CHD, including smoking, systolic and diastolic blood pressure, body weight, alcohol, and physical activity. The authors reported up to 83 different initial risk factors, but there was no mention of personality type. Did the authors and chief consultants discount this variable because it was not believed to be a possible valid confounding factor? In view of the voluminous literature on this risk factor and the effect of stress on serum lipid levels, how can this factor be discounted?
Deoxycytidine-kinase knockdown as a novel myeloprotective strategy in the context of fludarabine, cytarabine or cladribine therapy
Authors: N Lachmann, K Czarnecki, S Brennig, R Phaltane, M Heise, N Heinz, H Kempf, D Dilloo, V Kaever, A Schambach, M Heuser, T Moritz
Journal: Leukemia (2015)