tbuckley/cabot-search huggingface dataset:
Abstract 3025: Detection of prostatic fluid micro-RNA species associated with prostate cancer
Authors: Holly B. Lewis, Raymond S. Lance, Richard R. Drake, Aurora E. Kerscher
Journal: Cancer Research (2010)

Abstract There is a need to develop more accurate and specific biomarkers that can be used in the early detection and determination of aggressiveness of prostate cancers. Collection of expressed prostatic secretions (EPS) in urine can be done following digital rectal exam (DRE) and gentle massage of each side of the prostate gland. This massage stimulates the release and movement of prostatic fluids and detached epithelial cells into the urethra. EPS is currently being used to develop genetic screens for expression of PCA3 non-coding RNA and TMPRSS2/Erg fusion transcripts, and thus could also provide a rich source of prostate-derived microRNAs (miRNAs). This collection is easily obtained, not disruptive to a standard urological exam and can be collected from men at all stages of prostate cancer, thus making EPS an ideal material to develop diagnostic screens for prostate cancer. Proteins and nucleic acids obtained from these fluids are characteristic of the prostate because the fluid is largely devoid of seminal vesicle-derived proteins or sperm. An additional prostatic fluid from cancer patients, termed direct EPS, has been collected via the penis following a vigorous pre-prostatectomy exam while the patient is under anesthesia. Our laboratory has begun to profile the expression of EPS-derived miRNAs to identify novel prostate cancer biomarkers. Large sample numbers of pre-DRE and post-DRE EPS urine specimens from prostate cancer patients and non-cancer controls have been collected to facilitate identification of cancer-specific as well as prostate-specific miRNA biomarkers. We have optimized procedures to isolate and profile the expression of EPS-derived miRNAs using stem-loop RT followed by TaqMan PCR analysis from 0.2-0.5 mls of sample. Several miRNAs linked to cancer progression (i.e., let-7, miR-16, miR-17, miR-21) have been successfully profiled and show differential expression patterns in post-DRE EPS urine samples obtained from prostate cancer patients compared to non-cancer controls. For comparison, we have also identified a subset of miRNA genes that are enriched in prostate cancer cell lines exhibiting invasive metastatic characteristics as well as miRNAs that are enriched in non-invasive prostate cell lines. We are testing if the expression of these miRNA subclasses correlates with the disease states of our patient EPS specimens, with the goal of developing miRNA signatures that could be used as new diagnostic and prognostic biomarkers for prostate cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3025.
Human Serum Paraoxonases (PON1) Q and R Selectively Decrease Lipid Peroxides in Human Coronary and Carotid Atherosclerotic Lesions
Authors: Michael Aviram, Emiliya Hardak, Jacob Vaya, Saeed Mahmood, Simcha Milo, Aaron Hoffman, Scott Billicke, Dragomir Draganov, Mira Rosenblat
Journal: Circulation (2000)

Human serum paraoxonase (PON1) exists in two polymorphic forms: one that differs in the amino acid at position 192 (glutamine and arginine, Q and R, respectively) and the second one that differs in the amino acid at position 55 (methionine and leucine, M and L, respectively). PON1 protects LDL from oxidation, and during LDL oxidation, PON1 is inactivated.The present study compared PON1 isoforms Q and R for their effect on lipid peroxide content in human coronary and carotid lesions. After 24 hours of incubation with PON1Q or PON1R (10 arylesterase units/mL), lipid peroxides content in both coronary and carotid lesion homogenates (0.1 g/mL) was reduced up to 27% and 16%, respectively. The above incubation was associated with inactivation of PON1Q and PON1R by 15% and 45%, respectively. Lesion cholesteryl linoleate hydroperoxides and cholesteryl linoleate hydroxides were hydrolyzed by PON1 to yield linoleic acid hydroperoxides and linoleic acid hydroxides. Furthermore, lesion and pure linoleic acid hydroperoxides were reduced to yield linoleic acid hydroxides. These results thus indicate that PON1 demonstrates esterase-like and peroxidase-like activities. Recombinant PON1 mutants in which the PON1-free sulfhydryl group at cysteine-284 was replaced with either alanine or serine were no longer able to reduce lipid peroxide content in carotid lesions.We conclude that PON1 may be antiatherogenic because it hydrolyzes lipid peroxides in human atherosclerotic lesions.
Albumin Microbubble Persistence During Myocardial Contrast Echocardiography Is Associated With Microvascular Endothelial Glycocalyx Damage
Authors: Jonathan R. Lindner, Suad Ismail, William D. Spotnitz, Danny M. Skyba, Ananda R. Jayaweera, Sanjiv Kaul
Journal: Circulation (1998)

Background —We hypothesized that the persistence of albumin microbubbles within the myocardium during crystalloid cardioplegia (CP) infusion and ischemia-reperfusion (I-R) occurs because of endothelial injury. Methods and Results —The myocardial transit rate of albumin microbubbles was measured in 18 dogs perfused with different CP solutions and in 12 dogs undergoing I-R. Electron microscopy with cationized ferritin labeling of the glycocalyx was performed in 9 additional dogs after CP perfusion and in 3 additional dogs undergoing I-R. Microbubble transit was markedly prolonged during crystalloid CP perfusion. The addition of whole blood to the CP solution accelerated the transit rate in a dose-dependent fashion ( P &lt;0.05), which was greater with venous than with arterial blood ( P &lt;0.05). The addition of plasma or red blood cells to CP solutions was less effective in improving transit rate than addition of whole blood ( P &lt;0.05). Microbubble transit rate was independent of the temperature, K + content, pH, P o 2 , osmolality, viscosity, and flow rate of the perfusate. Similarly, a proportion of microbubbles persisted in the myocardium after I-R, which was related to the duration of ischemia ( P &lt;0.01) but not of reflow. Crystalloid CP perfusion and I-R resulted in extensive loss of the endothelial glycocalyx without other ultrastructural changes. This effect was partially reversed in the case of crystalloid CP when it was followed by blood CP. Conclusions —Sonicated albumin microbubbles persist within the myocardium in situations in which the endothelial glycocalyx is damaged. The measurement of the myocardial transit rate of albumin microbubbles may provide an in vivo assessment of endothelial glycocalyx damage.
833. A Preclinical Safety Study of Simian Immunodeficiency Virus (SIV)-Based Lentivirus Vector for Retinal Gene Transfer in Non-Human Primates
Authors: Yasuhiro Ikeda, Yoshikazu Yonemitsu, Masanori Miyazaki, Riichiro Kono, Toshinori Murata, Yoshinobu Goto, Toshiaki Tabata, Yasuji Ueda, Naohide Ageyama, Keiji Terao, Mamoru Hasegawa, Tatsuro Ishibashi, Katsuo Sueishi
Journal: Molecular Therapy (2005)

Top of pageAbstract
Purpose: Recently, we have demonstrated the efficient and stable retinal gene transfer mediated by the non-pathogenic simian immunodeficiency virus (SIV)-based lentivirus vector as well as the therapeutic outcome in some animal models of retinal degeneration using recombinant SIV vectors carrying neurotrophic factors, such as pigment epithelium-derived factor (PEDF) and fibroblast growth factor-2 (FGF-2). Here, we report the acute ophthalmic toxicity following intraocular administration of SIV-PEDF in Macaca fascicularis, as a preclinical safety study.

Methods: Eleven Macaca fascicularis (body weight=2.35-5.63 kg) were enrolled in this study. Approximately 20 |[mu]|l of SIV-PEDF (low titer: 2.5|[times]|107 transducing units [TU]/ml, equal to clinically available titer, high titer: 2.5|[times]|108 TU)/ml, and max titer: 1.0|[times]|109 TU/ml, n=3, respectively) or control (balanced salt solution: BSS, n=2) were injected into subretinal space via a glass capillary tube. We undertook an ophthalmic examination including slitlamp biomicroscopy, intraocular pressure (IOP) measurement, fundoscopic examination, and fluorescein anigiography, and assessed transgene expression, retinal function with electroretinogram (ERG) and histology for 3 months.

Results: SIV vector was efficiently transferred mainly to the retinal pigment epithelium and transgene expression was detected within 3 months. Transient inflammatory cell infiltration to anterior ocular segment and elevation of IOP were observed in some animals, but not dose-dependent. ERGs including multi-focal ERGs revealed no remarkable change of the retinal functions. Retinas treated with both SIV-PEDF and BSS showed no significant inflammatory infiltration and retinal structural destruction. Furthermore, neither dead animal nor serious side effect was found during experimental course.

Conclusions: The current study indicated the acute local and systemic safety of intraocular administration of SIV-PEDF, even if it was given at 40-times higher titer than clinical available titer. We are now conducting the long-termed safety study in non-human primates.
Insulin sensitivity and vascular disease in maturity-onset diabetics
Authors: A. E. Stocks, F. I. R. Martin
Journal: BMJ (1969)

In 27 maturity-onset diabetics of more than 10 years' duration there was a significant tendency for both atherosclerosis and microangiopathy to be associated with a reduction in the acute hypoglycaemic effect of intravenous beef insulin. This finding is identical to that in a group of 51 insulin-dependent diabetics previously reported.It is suggested that insulin insensitivity probably precedes the appearance of vascular disease in diabetes and may be implicated in its production.
Oxygen-Saturation Targets and Outcomes in Extremely Preterm Infants
Authors: Lisa Maree Askie, David John Henderson-Smart, Les Irwig, Judy Margaret Simpson
Journal: New England Journal of Medicine (2003)

Physiological studies have shown that chronic hypoxemia may occur in preterm infants who require supplemental oxygen for extended periods and that this hypoxemia may contribute to poor growth and development. Anecdotal reports and uncontrolled observational studies have suggested that a higher oxygen-saturation range may be beneficial in terms of growth and development.
A Course in Epidemiology
Authors: Gaylord W. Anderson
Journal: JAMA (1961)

This is a translation of a Russian text on epidemiology that was published in 1958. Part 1, headed "General Epidemiology" and written by Professor Elkin, consists of 15 chapters on general principles of epidemiology and methods of infectious disease control. Part 2, labelled "Special Epidemiology," consists of a series of sections written by some half dozen collaborators and deals with 65 different infections, grouped as intestinal, respiratory tract, blood, and external surface infections. Notably missing are sections on staphylococcal infections, the common cold, adenovirus and ECHO virus infections, and the pneumonias, whether of viral or pneumococcal origin. The chapters on specific diseases are well done. While his collaborators have limited themselves to scientific observations, Professor Elkin has missed no opportunity to incorporate Communistic philosophy and glorification of the Soviet system, which he finds "is the best guarantee of successful solution of the problems which arise in the prophylaxis of infectious
Response to Letter Regarding Article, “Quantification of Incomplete Revascularization and Its Association With Five-Year Mortality in the Synergy Between Percutaneous Coronary Intervention With Taxus and Cardiac Surgery (SYNTAX) Trial: Validation of the Residual SYNTAX Score”
Authors: Vasim Farooq, Patrick W. Serruys, Christos V. Bourantas, Yaojun Zhang, Takashi Muramatsu, Ted Feldman, David R. Holmes, Michael Mack, Marie Claude Morice, Elisabeth Ståhle, Antonio Colombo, Ton de Vries, Marie-angèle Morel, Keith D. Dawkins, Arie Pieter Kappetein, Friedrich W. Mohr
Journal: Circulation (2014)
Tumors of the Patella
Authors: Paul H. Johanson
Journal: JAMA (1968)

To  Editor:—  In  QUESTION AND ANSWER section (205:193, 1968) David Dahlin, MD, responded to a question by Leonard Wachs, MD, regarding a possible osteochondroma of  patella. Dr. Dahlin quoted Jaffe's statement that cartilage-capped exostoses develop only in relation to bones preformed in cartilage. Dr. Dahlin then stated that the patella is not in this category and implies that for this reason  lesion in question was not an osteochondroma.  There is a cartilaginous precursor of  patella. Standard anatomy texts indicate that  cartilaginous precursor is formed during  third intra-uterine month and usually ossifies by about  third year of life.1-3  Jaffe also states that  enchondroma occurs only in bones preformed in cartilage.4Linscheid and Dahlin reported a chondroma (enchondroma) of  patella in 1966.5  Possibly osteochondromata do not occur on  patella but certainly not for  reason given by
Blood Groups in Man
Authors: Frances K. Widmann
Journal: JAMA (1976)

A golden era, a silver anniversary. The first edition of<i>Blood Groups in Man</i>appeared in 1950. The 1975 edition, the sixth of its line, is described by the authors as "the last...: the subject has grown to need more than our two pencils." Within its steadily expanding covers, this volume has, for a quarter century, compiled, analyzed, and enriched the world literature on the entire field of red blood cell antigens. In 1950, two hundred seventy-six pages were sufficient to discuss all the known blood group systems: ABO, Lewis, Rh, Kell, Lutheran, P, and MNS. The newest edition devotes chapter titles to 16 blood systems, not to mention innumerable antigens, surface characteristics, and serologic phenomena organized under other headings. There may well be one or two topics or studies the authors have overlooked, but<i>Blood Groups in Man</i>remains the all-inclusive, remarkably comprehensible reference work no blood banker, immunohematologist,
POST PARTUM HÆMORRHAGE.Read before the Milwaukee Medical Societv, May 10. 1892.
Authors: S. W. FRENCH
Journal: JAMA (1892)

Perhaps I should apologize to the society for bringing before them a subject which at first sight seems so simple; but post partem hæmorrhage does not come under our hands', fortunately, very often, but when we do encounter it, it is a condition that needs immediate attention, and no delaying or expectant plan whatsoever. I therefore bring up the subject more for discussion than for any intrinsic value that the paper may have to the society. It has been my fortune during a practice of thirteen years in this city to meet with but few cases of post partem hæmorrhage. Of course we often meet with cases that are of a slight character, which after a thorough massage of the uterus, or a good free injection of hot water, stop within a short time. But I refer to cases where the hæmorrhage is particularly severe. The only really serious case
Which Colonic Adenomas Became Malignant?
Authors: Alfred I. Neugut
Journal: Annals of Internal Medicine (1993)

Letters1 August 1993Which Colonic Adenomas Became Malignant?Alfred I. Neugut, MD and Judith S. Jacobson, MDAlfred I. Neugut, MDSearch for more papers by this author and Judith S. Jacobson, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-119-3-199308010-00027 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:We agree with Koretz [1] that the evidence regarding the adenoma-carcinoma sequence is circumstantial, that malignant polyps may have a long latency period, that some colorectal cancer appears to arise de novo, and that the similarity in the distribution of adenomas and cancer may be caused by a field defect in the colorectal mucosa that gives rise to both.However, other factors need to be considered as well. The prevalence of adenomas found at autopsy in different populations is highly correlated with cancer incidence (r = 0.67). The mean age for cancer is approximately 7 years older ...References1. Koretz RL. Malignant polyps: are they sheep in wolves' clothing? Ann Intern Med. 1993; 118:63-8. Google Scholar2. Neugut AI, Jacobson JS, DeVivo I. The epidemiology of colorectal adenomatous polyps. Cancer Epidemiol Biomarkers Prev. 1993; 2: 159-76. Google Scholar3. Neugut AI, Garbowski G, Lee WC, Murray T, Nieves JW, Forde KA, et al. Dietary risk factors for the incidence and recurrence of colorectal adenomatous polyps. Ann Intern Med. 1993; 118:91-5. Google Scholar4. Stryker SJ, Wolff BG, Culp CE, Libbe SD, Ilstrup DM, MacCarty RL. Natural history of untreated colonic polyps. Gastroenterology. 1987; 93:1009-13. Google Scholar Author, Article, and Disclosure InformationAuthors: Alfred I. Neugut, MD; Judith S. Jacobson, MD PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byProspektive klinische Studie zur Sicherheit von Anthranoidlaxanzien 1 August 1993Volume 119, Issue 3Page: 250-252KeywordsAdenomasCancer preventionCancer risk factorsCancer screeningColorectal cancerMedical risk factorsMucous membranesPolypectomiesPopulation statisticsRelative risk ePublished: 15 August 2000 Issue Published: 1 August 1993 Copyright & PermissionsCopyright © 1993 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Clinicians' roles in management of arsenicosis in Bangladesh: interview study
Authors: Rubaiul Murshed, Robert M Douglas, Geetha Ranmuthugala, Bruce Caldwell
Journal: BMJ (2004)

mon midwifery practice.This multicentre randomised controlled trial did not support the effectiveness of this intervention.Given the study design involving seven different units, these results would probably be applicable to other populations.Therefore, in the absence of evidence of a beneficial effect, we would suggest that this advice should be discontinued, at least as a way of changing the fetal position.We thank the patients and the secretarial and medical staff of the participating hospitals.We also thank Di Wilton for help in recruitment and in collecting and entering the data, the midwives for their support, Alan Brnabic for his help with the analyses, and Jack Cheng for his advice on our database.
Appendix abscess with intestinal haemorrhage.
Authors: P J Milewski
Journal: BMJ (1977)

of gross marrow activity.A few days after the child was admitted to hospital his mother developed a sore throat and follicular tonsillitis.Group A r- haemolytic streptococci were isolated from the mother's throat, the grandmother's nose, and the nose of a nursery nurse in the maternity department.The streptococci from the patient, his mother, and his grandmother were indistinguishable by typing (M3.R3.T3/13/B3264) but were different from the nurse's strain (M4.R-T4).
IL-25 Induces IL-4, IL-5, and IL-13 and Th2-Associated Pathologies In Vivo
Authors: Madeline M. Fort, Jeanne Cheung, David Yen, Joana Li, Sandra M. Zurawski, Sylvia Lo, Satish Menon, Teresa Clifford, Brisdell Hunte, Robin Lesley, Tony Muchamuel, Stephen D. Hurst, Gerard Zurawski, Michael W. Leach, Daniel M. Gorman, Donna M. Rennick
Journal: Immunity (2001)
The epidemiology of scabies in an impoverished community in rural Brazil: Presence and severity of disease are associated with poor living conditions and illiteracy
Authors: Hermann Feldmeier, Anne Jackson, Liana Ariza, Cláudia Maria Lins Calheiros, Valquiria de Lima Soares, Fabíola A. Oliveira, Ulrich R. Hengge, Jörg Heukelbach
Journal: Journal of the American Academy of Dermatology (2008)
Use of lectins for the study of human epidermal differentiation
Authors: A. REANO, M. FAURE, Y. JACQUES, U. REICHERT
Journal: British Journal of Dermatology (1982)

Journal Article Use of lectins for the study of human epidermal differentiation Get access A. REANO, A. REANO U. 209 INSERM, Clinique Dermatologique, Hôspital E. Herriot, Lyon Search for other works by this author on: Oxford Academic Google Scholar M. FAURE, M. FAURE U. 209 INSERM, Clinique Dermatologique, Hôspital E. Herriot, Lyon Search for other works by this author on: Oxford Academic Google Scholar Y. JACQUES, Y. JACQUES CIRD, Sophia Antipolis, Valbonnc, France Search for other works by this author on: Oxford Academic Google Scholar U. REICHERT U. REICHERT CIRD, Sophia Antipolis, Valbonnc, France Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 107, Issue s23, 1 November 1982, Pages 143–146, https://doi.org/10.1111/j.1365-2133.1982.tb01047.x Published: 01 November 1982
Spontaneous abortion after hand-foot-and-mouth disease caused by Coxsackie virus A16.
Authors: M M Ogilvie, C F Tearne
Journal: BMJ (1980)

Azoospermia after iodine-131 treatment for thyroid carcinoma Male infertility from gonadal damage caused by irradiation and cyto- toxic chemotherapy is seldom reversible,' 2 and therapeutic strategies include cryostorage of semen, gonadal shielding, and surgical oophoro- pexy.Thyroid carcinoma may occur at any age,3 and operation, thyroxine suppression, and treatment with radioiodine (13"I) usually result in long-term survival.The efficacy and safety of 131I are well established, though leukaemia, marrow depression, and radiation pneumonitis are rare complications.3Infertility after 131I for carcinoma of the thyroid has not been reported.
The Bus at Night
Authors: George N. Braman
Journal: Annals of Internal Medicine (2007)

Ad Libitum20 March 2007The Bus at NightGeorge N. Braman, MDGeorge N. Braman, MDFrom Bronx, NY 10471.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-146-6-200703200-00009 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail “It is the blight man was born for,It is Margaret you mourn for.”—To a Young Child, Gerard Manley Hopkins... Author, Article, and Disclosure InformationAffiliations: From Bronx, NY 10471.Current Author Address: George N. Braman, MD, 6142 Delafield Avenue, Bronx, NY 10471. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 20 March 2007Volume 146, Issue 6Page: 442KeywordsFearGames ePublished: 20 March 2007 Issue Published: 20 March 2007 Copyright & PermissionsCopyright © 2007 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Polymyositis as a cause of total gut failure
Authors: A J Hughes
Journal: Annals of the Rheumatic Diseases (2002)

Gastrointestinal manifestations are seen in systemic sclerosis and mixed connective tissue disorders but are rare in pure polymyositis.A 44 year old woman with polymyositis who developed total gut failure requiring treatment with total parenteral nutrition is described.The patient's polymyositis is now fully controlled biochemically, but her gastrointestinal symptoms persist.