tbuckley/cabot-search huggingface dataset:
P739Phosphodiesterase 5A expression is up-regulated in vascular endothelium under pro-inflammatory conditions: a newly disclosed anti-inflammatory activity by the omega-3 fatty acid docosahexaenoic acid
Authors: M Massaro, E Scoditti, MA Pellegrino, N Calabriso, MA Carluccio, C Storelli, R De Caterina
Journal: Cardiovascular Research (2014)

Endothelial inflammatory dysfunction, reflected by reduced NO bioavailability, is recognized as promoting atherosclerosis. NO activates the soluble guanylate cyclase (sGC) and the subsequent generation of cyclic guanosine monophosphate (cGMP), which in turn, serves as a final modulator of vascular relaxation. PDE5A, is a phosphodiesterase that catalyses the hydrolysis of cGMP into GMP, thus curtailing NO signalling and favouring pro-angiogenic effects. The ω-3 fatty acids DHA and eicosapentaenoic acid (EPA) are considered health-promoting nutrients. However, molecular mechanisms underlying their effects remain incompletely understood. Since recent data suggest a role for endothelial phosphodiesterase (PDE)5A in the modulation of endothelial-dependent vasodilation and angiogenesis, we investigated whether inflammatory stimuli known to be involved in the inflammation-mediated endothelial dysfunction and angiogenesis affect the endothelial PDE5A expression, and whether cell exposure to DHA modifies such PDE5A expression. Methods: Human umbilical vein endothelial cells (HUVEC) were treated with inflammatory or pro-angiogenic stimuli interleukin (IL)-1, tumour necrosis factor (TNF), IL-6, vascular endothelial growth factor (VEGF) and phorbol myristate acetate (PMA) for 0-24 hours. After this time, PDE5A expression was assessed by Western analysis, while mRNA expression was investigated by quantitative PCR (qPCR). In a subset of experiments HUVEC were treated with 0-50 μmol/L DHA for 48 hours before stimulation with IL-1, and PDE5A expression was then evaluated by Western analysis and qPCR. Results: PDE5A protein expression increased significantly after stimulation with 10 ng/mL IL-1 and TNF for 12 h (P<0.01 vs control), but not after stimulation with IL-6, PMA and VEGF. At the same concentrations, IL-1 also increased mRNA expression by 40% (P<0.05 vs control). DHA treatment of HUVEC before IL-1 stimulation reduced PDE5A induction at both protein and mRNA levels (P<0.05 vs IL-1). Conclusions: Classical pro-inflammatory - but not specifically pro-angiogenic - stimuli significantly induce PDE5A expression in endothelial cells, thus suggesting the involvement of an inflammatory signaling in the mechanisms leading to PD5A expression. Since PDE5A inhibitors are now approved for use in erectile dysfunction and pulmonary hypertension and have a potential in treating other disease states featuring endothelial dysfunction, downregulating the inflammation-mediated expression of PDE5A by DHA may positively impact the NO/sGC/cGMP axis and reproduce, at least partially, the therapeutic potential of PDE5 inhibitors.
Registration of Clinical Trials
Authors: Christine Laine, Harold C. Sox
Journal: Annals of Internal Medicine (2005)

Letters1 February 2005Registration of Clinical TrialsChristine Laine, MD, MPH, Senior Deputy Editor and Harold C. Sox, MD, EditorChristine Laine, MD, MPH, Senior Deputy EditorSearch for more papers by this author and Harold C. Sox, MD, EditorSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-142-3-200502010-00027 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail IN RESPONSE:Drs. Braillon, Dubois, and Slama raise 2 concerns about the policy on trial registration that the ICMJE announced in September 2004: the ICMJE definition of a clinical trial and the criteria of acceptable registries. For the purposes of the policy, the ICMJE defines a clinical trial as “any research project that prospectively assigns human subjects to intervention or comparison groups to study the cause-and-effect relationship between a medical intervention and a health outcome.” This definition focuses the policy on studies with the most immediate potential relevance to clinical practice, which are also the studies for which selective reporting ... Author, Article, and Disclosure InformationAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoClinical Trial Registration: A Statement from the International Committee of Medical Journal Editors Catherine De Angelis , Jeffrey M. Drazen , Frank A. Frizelle , Charlotte Haug , John Hoey , Richard Horton , Sheldon Kotzin , Christine Laine , Ana Marusic , A. John P.M. Overbeke , Torben V. Schroeder , Harold C. Sox , and Martin B. Van Der Weyden Registration of Clinical Trials Alain Braillon , Gérard Dubois , and Michel Slama Metrics 1 February 2005Volume 142, Issue 3Page: 228KeywordsClinical trials ePublished: 1 February 2005 Issue Published: 1 February 2005 CopyrightCopyright © 2005 by American College of Physicians. All Rights Reserved.PDF DownloadLoading ...
"SYMPTOMLESS ROOT-CANAL-FILLED TEETH"
Authors: H. A. Tuckey
Journal: JAMA (1928)

<h3>To the Editor:</h3> —The interesting paper presented by Dr. A. G. Gould on "Symptomless Root-Canal-Filled Teeth," inThe Journal, May 12, bears answering from an unprejudiced, scientific point of view. The medical profession, and also the dental profession for that matter, should take the position that every pulpless tooth, with or without symptoms, is a potential if not an actual source of infection. The use of the roentgen ray, generally, merely shows whether the tooth is vital or nonvital. It should be obvious that mere macroscopic conditions, seen with the roentgen ray, mean nothing. One cannot observe bacteriologic or pathologic changes without the aid of a microscope; hence the rendering of opinions from roentgen-ray evidence that teeth are negative or positive is worthless. Dr. Gould in his summary makes the statement: "Dentists do not agree as to the treatment of pulpless teeth." There can be no scientific method evolved for
Influence of biliary cirrhosis on the detoxification and elimination of a food derived carcinogen
Authors: C G Dietrich, A Geier, H E Wasmuth, S Matern, C Gartung, D R de Waart, R P J O Elferink
Journal: Gut (2004)

<b>Background and aims:</b> The liver is the central organ for the detoxification of numerous xenobiotics, including carcinogens. We studied the influence of cholestasis and biliary cirrhosis on the detoxification, elimination, and tissue distribution of a model compound and food derived carcinogen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). <b>Methods:</b> Wistar rats were injected with <sup>14</sup>C-PhIP into the portal vein one or six weeks after common bile duct ligation (CBDL). Bile flow was reconstituted, bile and urine were collected over 120 minutes, and metabolites were analysed using high performance liquid chromatograpy. Total tissue radioactivity levels in several organs as well as tissue bound (ethanol insoluble tissue fraction) radioactivity levels were determined. <b>Results:</b> Significant downregulation of the transport proteins multidrug resistance associated protein 2 and breast cancer resistance protein was observed in biliary cirrhosis. Biliary excretion of radioactivity was significantly reduced in cholestasis and biliary cirrhosis compared with controls (15 (2.9)% and 3.2 (1)% of the dose <i>v</i> 36.5 (2)%, respectively). Phase II metabolism was severely reduced in cirrhotic rats, resulting in a twofold increase in tissue radioactivity levels in the liver, kidney, and colon. Biliary cirrhosis increased tissue binding of reactive metabolites, as expressed in cpm/100 mg tissue in the liver and the colon (3267 (1218) <i>v</i> 1191 (429) in the liver, 3044 (1913) <i>v</i> 453 (253) in the colon). <b>Conclusions:</b> Biliary cirrhosis induced by CBDL causes impaired metabolism and elimination of PhIP, and leads to higher tissue levels of potentially genotoxic metabolites in the liver and colon of rats. These data may explain the increased incidence of hepatic and extrahepatic cancers in cholestasis and liver cirrhosis.
Royal college is forced to retreat on star rating system for GP clinics
Authors: Madeleine Brettingham
Journal: BMJ (2006)

The Royal College of General Practitioners announced last week that it was suspending talks with the Department of Health after an outcry over an article in The Times (6 Jun, p 1) that claimed that the college was planning to introduce a star rating system for general practices.

The chairman of the college, Mayur Lakhani, said: “In light of the recent feedback we have had and the anxieties that GPs feel, I have today decided to review the project and suspend discussions with the Department of Health.”

Professor Lakhani expressed dismay that the leaks—which claimed that …
Rheumatoid arthritis: extra-articular manifestations.
Authors: A. Calin
Journal: BMJ (1969)

Important circumstantial evidence in support of the theory of passive dilatation of the ventricle is provided by consideration of the abnormal but characteristic ventricular cardiographic silhouettes after mitral valve replacement using low-profile disc and central-flow homograft valves.As a result of some preliminary observations we would distinguish the " turnip " heart outline that characterizes the disc valves from the " pear " heart of the homograft valve, and suggest that the shape adopted by the ventricle is determined by the grossly abnormal filling patterns in each case."It seems logical that the reduction in cardiac output that is uni- formly observed in these patients is related directly to these changes.-Iam, etc.,
Norwegian Scabies — Report and Lessons of a Case
Authors: Stephen B. Kurtin, Morris Leider
Journal: New England Journal of Medicine (1968)

SO-CALLED Norwegian scabies is an infestation with Sarcoptes scabiei var. hominis that is clinically different from ordinary scabies in its extensiveness, the kind of lesion produced and the type of patient who suffers it. First described in 1848 by Danielssen and Boeck1 in a leper, the condition was named "scabies norvegica Boeckii" by Hebra2 in 1851 when he was shown another case, which he and Boeck proved, by a simple transfer experiment, to be caused by the same mite that causes ordinary scabies. In the United States, only five cases of Norwegian scabies had been reported to 1964.3 4 5 6 7 Case Report . . .
[664] DETECTION OF MND AND RL/M ANTI-NUCLEAR ANTIBODIES PATTERNS: A RELIABLE DIAGNOSTIC TOOL IN ANTI-MITOCHONDRIAL ANTIBODIES NEGATIVE PRIMARY BILIARY CIRRHOSISd
Authors: A. Granito, G. Pappas, P. Muratori, L. Muratori, S. Ferri, C. Quarneti, C. De Molo, V. Cipriano, F. Cassani, J. Worthington, R.W. Chapman, F.B. Bianchi
Journal: Journal of Hepatology (2007)
Intravenous Digital Subtraction Angiography and Lung Imaging: Compared Value in the Diagnosis of Pulmonary Embolism
Authors: JEAN ROSSO, DOMINIQUE MUSSET, PATRICK PETITPRETZ, JANINE MENSCH, PIERRE DUROUX, PIERRE GALLE, MICHEL MEIGNAN
Journal: Clinical Nuclear Medicine (1989)

In a prospective study, 42 consecutive patients with clinically suspected pulmonary embolism underwent ventilation-perfuston (V-Q) lung Imaging and digital subtraction anglography (DSA) concurrently with selective conventional pulmonary anglography (CPA). Thirty-eight studies achieved within 24 hours were reviewed independently by two pairs of observers. The findings were compared using CPA as the gold standard. V-Q lung Imaging had a high percentage of indeterminate results, but none were false negative nor false positive. DSA had a lower percentage of indeterminate results but missed four of the 25 positive cases and erroneously affirmed the presence of pulmonary embolism In three cases. Therefore, the authors think that V-Q lung imaging should remain the screening examination of choice for evaluating patients with suspected pulmonary embollsm.
Disc Space Infections: Early Reports in French
Authors: M. F. KAHN
Journal: Annals of Internal Medicine (1978)

Letters and Corrections1 September 1978Disc Space Infections: Early Reports in FrenchM. F. KAHN, M.D.M. F. KAHN, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-89-3-428_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: When I read in the excellent paper of Drs. Churchill, Geraci, and Hunder (Ann Intern Med 87:754-759, 1977) that "disc space infections have not been reported in endocarditis previously," I only smiled and decided to not react. But the letter by W. J. Untereker and B. A. Hanna (Ann Intern Med 88:578, 1978) asking for recognition of an earlier description prompted me to write.To my knowledge, the first complete description of disc space infection in endocarditis was given by our team as early as 1965 (1) with the report of two cases. Further, no less than...References1. SEZERYCKEWAERTKAHNSIMONINHEULEU SDAMJJ: L'endocardite d'Osler en rhumatologie. Rev Rhum Mal Osteoartic 32:739-744, 1965 MedlineGoogle Scholar2. CHOUBRACMATHÉHAASCRICKXROBINDYANDUBOIS PGCBPAG: Spondylodiscites révélatrices d'endocardites bactériennes. Ann Med Interne (Paris) 127:117-121, 1976 MedlineGoogle Scholar3. DESHAYESDURANDHOUDENTHUMBERTBRETON PJGGG: A propos des manifestations rhumatologiques des endocardites bactériennes. Rev Rhum Mal Osteoartic 41:135-141, 1974 MedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Hopital Bichat 170, bd Ney; 75018 Paris France PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited bySevere Backache as a Presenting Sign of Bacterial Endocarditis 1 September 1978Volume 89, Issue 3Page: 428-428KeywordsEndocarditis Issue Published: 1 September 1978 PDF downloadLoading ...
Chronic monotherapy with extended release metoprolol succinate attenuates mRNA gene expression for MMP2 and MMP9 in dogs with heart failure
Authors: Sharad Rastogi, Ramesh C. Gupta, Sudhish Mishra, Hideaki Morita, George Suzuki, Sidney Goldstein, Hani N. Sabbah
Journal: Journal of the American College of Cardiology (2003)
Traditional rulers in northern Nigeria call for halt to polio vaccination
Authors: Abiodun Raufu
Journal: BMJ (2004)

The controversy over the administration of poliomyelitis vaccines in northern Nigeria has taken a new turn as traditional rulers from the region have asked the Nigerian government to stop administering the vaccine because of fears that it is contaminated.

Speaking under the aegis of Jama'atul Nasril Islam (JNI), the umbrella organisation of Muslims in northern Nigeria, the rulers said their advice is based on the report of a medical team sent to India by the Muslim organisation to test …
Ciprofloxacin in primary prophylaxis of spontaneous bacterial peritonitis: A randomized, placebo-controlled study
Authors: Rubén Terg, Eduardo Fassio, Mónica Guevara, Mariano Cartier, Cristina Longo, Romina Lucero, Cristina Landeira, Gustavo Romero, Nora Dominguez, Alberto Muñoz, Diana Levi, Carlos Miguez, Raquel Abecasis
Journal: Journal of Hepatology (2008)
Chaos in induced rhythms of the brain – the value of ERP studies
Authors: Márk Molnár
Journal: Behavioral and Brain Sciences (1996)

Abstract Event-related potentials (ERPs) – neglected almost entirely by Wright &amp; Liley – allow objective investigation of information processing in the brain. The application of chaos theory to such an analysis broadens this possibility. Through the use of the point correlation dimension (PD2) accurate dimensional analysis of different Event-Related Potential components such as the P3 wave is possible.
Surgery for Reflux Disease — Reflections of a Gastroenterologist
Authors: Joel E. Richter
Journal: New England Journal of Medicine (1992)

Gastroesophageal reflux is one of the most prevalent clinical conditions affecting the gastrointestinal tract. Its spectrum ranges from the postprandial heartburn experienced at times by most adults to severe, complicated disease characterized by ulcerative esophagitis, esophageal strictures, and Barrett's esophagus (replacement of squamous epithelium with columnar epithelium) with its tendency to become malignant. Cross-sectional surveys of presumably healthy subjects in the United States have found that 7 to 10 percent have heartburn daily and 15 to 40 percent have it monthly.1 , 2 Approximately one third of the patients with heartburn who seek medical care have endoscopic evidence of esophagitis, and about . . .
Development of an 125I-postlabeling assay as a simple, rapid, and sensitive index of DNA-protein cross-links.
Authors: Z Zhuang, M Costa
Journal: Environmental Health Perspectives (1994)

A rapid, simple, and sensitive 125I-postlabeling technique has been developed to allow detection of DNA-protein cross-links induced by environmental contaminants and carcinogens. This method is based on specific incorporation of 125I into tyrosine residues associated with DNA. Cultured Chinese hamster ovary cells were exposed to various crosslinking agents, e.g., UV light, K2CrO4, or NiCl2. DNA was isolated by proteinase K/phenol/chloroform. The residual peptides cross-linked to DNA were radioiodinated with Na125I and chloramine T. After repeated precipitation with ethanol, the radioactivity was determined. The 125I method was compared with a 3[H]-tyrosine prelabeling method and found to be of similar sensitivity.
Epidemic Neuromyasthenia
Authors: David C. Poskanzer, Donald A. Henderson, E. Charles Kunkle, Seymour S. Kalter, Walter B. Clement, James O. Bond
Journal: New England Journal of Medicine (1957)

AN obscure epidemic illness occurred in a small Florida community in the spring of 1956. It was characterized principally by fatigue, headache, nuchal pain, alterations in emotional status and mentation, nausea and vomiting, paresthesias, aching muscular pain and a prolonged, relapsing course. Symptoms were protean, and the problems of case and epidemic delineation of this illness were manifold.The entity is similar to that described by various authors, particularly in recent years, from many different parts of the world. It has been termed "Iceland disease,"1 "benign myalgic encephalomyelitis,"2 "Akureyri disease"3 and "epidemic vegetative neuritis."4 It is reported as having occurred . . .
Human Leptospirosis from Immunized Dogs
Authors: RALPH D. FEIGIN
Journal: Annals of Internal Medicine (1973)

Leptospira icterohaemorrhagiae was isolated from the urine of a critically ill patient from suburban St. Louis. Additional cases among close contacts of the patient and the animal source of the infection were sought. Four additional human infections with L. icterohaemorrhagiae were discovered. Four healthy immunized dogs that were pets of the infected patients and that had access to land on which rats had been seen were shown to be infected with the same organism. Two of these animals were leptospiruric when they were sampled. Nine rats were trapped in the sewers on the street where the human and canine cases were discovered. All nine rats were infected with L. icterohaemorrhagiae. When infection of rats with L. icterohaemorrhagiae is prevalent, canine infection and leptospiruria may occur even in healthy immunized animals, with subsequent development of the disease in humans.
965. Lymphatic and Systemic Metastasis of Prostate Cancer Induced by VEGF-C
Authors: Jeremy B. Burton, Ebba Brakenhielm, Saul J. Priceman, Nelson Chavarria, Kari Alitalo, Lily Wu
Journal: Molecular Therapy (2006)

Introduction: Metastatic disease is a major cause of prostate cancer mortality. Lymph node involvement is an important early prognostic indicator of patient survival. The extent of tumor- associated lymphangiogenesis can dictate lymphatic metastasis in many tumors. Limited experience on this issue in prostate cancer leads us to study tumor models, which recapitulate many of characteristics of the human disease including androgen regulation and PSA expression. Results: By applying in vivo bioluminescence imaging, we observed that prostate cancer xenografts display distinctive metastatic potentials. Whereas LAPC-4 and CWR22RV- 1 tumors metastasized to lymph nodes and lungs, LAPC-9 tumors did not. Quantitative real-time PCR revealed that the expression of pro-lymphangiogenic factor, VEGF-C, was elevated in LAPC-4 and CWR22RV-1 compared to LAPC-9 tumors. Immunohistochemistry showed that LAPC-4 tumors contained patent intratumoral lymphatics, often filled with tumor cells. To examine the correlation of tumor lymphangiogenesis and metastasis, we over-expressed VEGF-C in the non-metastatic LAPC-9 model, and we down- regulated lymphangiogenesis in CWR22RV-1 by expressing soluble VEGF-R3 to sequester VEGF-C. Recombinant lentiviral vectors were applied to express luciferase and vascular growth factor genes in the tumors. The effects of VEGF-C, VEGF-CC156S (a point mutant of VEGF-C that induces lymphangiogenesis but not angiogenesis), or VEGF-A were compared to control vector. While VEGF-A increased the number of blood vessels and the rate of growth, we observed limited enhancement of lymph node (signals 10-fold greater photons/s/cm2/sr over control) and lung metastasis. On the other hand, VEGF-CC156S and VEGF-C over-expression resulted in enhanced intratumoral lymphangiogenesis and significant dissemination to ipsilateral lymph nodes (200-to 1000-fold over control) and lungs (25-fold enhancement). The VEGF-CC156S tumors exhibited no increase in blood vessels. The expression of soluble VEGF-R3 in CWR22RV-1 resulted in a significant reduction in intratumoral vasculatures as well as metastasis to both regional lymph node and lung. The extent of metastasis in all tumor models assessed by optical imaging correlated well with immunocytochemical analyses of metastasis by cytokeratin marker and GFP. Conclusion: Bioluminescence imaging is a valuable tool to track metastasis in both the VEGF-C and VEGF-A expressing tumors. Our results indicate that tumor lymphangiogenesis is a key factor contributing to not only local regional lymph nodes but also to lungs in prostate cancer models. Inhibiting tumor lymphangiogenesis may be a promising therapeutic strategy to suppress the deadly consequence of systemic metastasis.
TCT-241 Value of Optical Coherence Tomography beyond the Napkin-Ring Sign in CT Angiography for Detecting Coronary Lipid-Core Plaques as Determined by Histology: A Multimodality Imaging Study in Human Donor Hearts
Authors: Christopher Schlett, Pál Maurovich-Horvat, Fabian Bamberg, William Warger, Masataka Nakano, Atsushi Tanaka, Marc Vorpahl, Harald Seifarth, Maros Ferencik, Renu Virmani, Guillermo Tearney, Udo Hoffmann
Journal: Journal of the American College of Cardiology (2012)