tbuckley/cabot-search huggingface dataset:
Lentiviral Vector Platform for Production of Bioengineered Recombinant Coagulation Factor VIII
Authors: H Trent Spencer, Gabriela Denning, Richard E Gautney, Boro Dropulic, Andre J Roy, Lajos Baranyi, Bagirath Gangadharan, Ernest T Parker, Pete Lollar, Christopher B Doering
Journal: Molecular Therapy (2010)

Patients with hemophilia A present with spontaneous and sometimes life-threatening bleeding episodes that are treated using blood coagulation factor VIII (fVIII) replacement products. Although effective, these products have limited availability worldwide due to supply limitations and product costs, which stem largely from manufacturing complexity. Current mammalian cell culture manufacturing systems yield around 100 µg/l of recombinant fVIII, with a per cell production rate of 0.05 pg/cell/day, representing 10,000-fold lesser production than is achieved for other similar-sized recombinant proteins (e.g. monoclonal antibodies). Expression of human fVIII is rate limited by inefficient transport through the cellular secretory pathway. Recently, we discovered that the orthologous porcine fVIII possesses two distinct sequence elements that enhance secretory transport efficiency. Herein, we describe the development of a bioengineered fVIII product using a novel lentiviral-driven recombinant protein manufacturing platform. The combined implementation of these technologies yielded production cell lines that biosynthesize in excess of 2.5 mg/l of recombinant fVIII at the rate of 9 pg/cell/day, which is the highest level of recombinant fVIII production reported to date, thereby validating the utility of both technologies.
Sudden collapse of a young female cross country runner
Authors: A S Kashyap, K P Anand, S Kashyap
Journal: British Journal of Sports Medicine (2006)

The case is reported of a young previously healthy female cross country runner who collapsed on completion of a cross country run. The cause of the collapse was non-cardiogenic pulmonary oedema as a manifestation of hyponatraemic encephalopathy. The concurrent occurrence of non-cardiogenic pulmonary oedema and encephalopathy due to hyponatraemia is unusual.
Differences in the Allergic Response Induced by Live or Dead Anisakis Simplex Larvae in C3H/HeJ Mice
Authors: M.L. Baeza, Y. Higaki, L. Conejero, A. Torrecilla, M. Vazquez de la Torre, M. Rubio, J.M. Zubeldia
Journal: Journal of Allergy and Clinical Immunology (2007)
Abstract 492: Hepatic oxidative stress activates the Gadd45b gene via degradation of the transcriptional repressor STAT3
Authors: Jung-Hwan Kim, Aijuan Qu, Janardan K. Reddy, Bin Gao, Frank J. Gonzalez
Journal: Cancer Research (2014)

Abstract Growth arrest and DNA damage-inducible beta (GADD45b) plays an important role in many intracellular events, such as cell cycle arrest, DNA repair, cell survival, apoptosis, and senescence. However, its mechanism of transcriptional regulation remains unclear. In this study the mechanism of proliferator-activated receptor a (PPARa) ligand induction of the Gadd45b gene in mouse liver was investigated. Gadd45b messenger RNA (mRNA) was markedly induced by the PPARa agonist, Wy-14,643, in wild-type mice but not in Ppara-null mice. Signal transducer and activator of transcription 3 (STAT3) was found to be a repressor of the Gadd45b gene through binding to upstream regulatory elements. The role of STAT3 in control of Gadd45b was confirmed using liverspecific Stat3-null mice. Wy-14,643 treatment stimulated STAT3 ubiquitination leading to activation of the Gadd45b gene as a result of loss of Gadd45b repression by STAT3. STAT3 degradation was induced by forced overexpression of the PPARa target geneencoded enzyme ACOX1, which produces increased H2O2 as a byproduct of fatty acid b-oxidation. H2O2 also stimulated expression of Gadd45b in cultured cells. In conclusion, these studies revealed that PPARa indirectly induces the Gadd45b gene in liver through promoting degradation of the repressor STAT3 as a result of elevated oxidative stress. Note: This abstract was not presented at the meeting. Citation Format: Jung-Hwan Kim, Aijuan Qu, Janardan K. Reddy, Bin Gao, Frank J. Gonzalez. Hepatic oxidative stress activates the Gadd45b gene via degradation of the transcriptional repressor STAT3. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 492. doi:10.1158/1538-7445.AM2014-492
Loss of P2Y14 results in an arresting response to hematological stress
Authors: Brian S. Garrison, Derrick J. Rossi
Journal: Journal of Clinical Investigation (2014)

The regenerative capacity of tissues to recover from injury or stress is dependent on stem cell competence, yet the underlying mechanisms that govern how stem cells detect stress and initiate appropriate responses are poorly understood. In this issue of the JCI, Cho and Yusuf et al. demonstrate that the purinergic receptor P2Y14 may mediate the hematopoietic stem and progenitor cell regenerative response.
A perfect death?
Authors: J. Morris
Journal: BMJ (2003)

This is my experience of death. My husband, David, died in November 2002 in his home at the age of 66. I am 54. He died on his bed, alone. The day before he died was beautiful. We had walked along a steep part of Offa's dyke, near Knighton. He had no angina and was quite fit.

David had retired in 2000 from running a stressful architectural practice. I was doing locum work, which enabled us to travel widely. He walked the dog each day and was turning into an expert in vegetable growing. He did not smoke …
GROWTH RECOVERY AFTER INHIBITION BY LEAD
Authors: F. S. Hammett
Journal: BMJ (1929)

IHIITOBYLA.IMDA JORM adrenialine 1 in 1,000 were giveni intramusculaily.In spite of these nmeasures he passed, half anl hour later (1.10 p.m.), witlhout fits, into a state of counplete comw.The pulse was slow and irregular, gLid tlle breathipig stertorous, No aceton,e could be detected in tlhe breath.Tlle pupils reacted to ligist, anid were isot dilatef.The knee-jerks were brisk, anid the plaiutar response of botlh feet was defllitely extensor.A catlheteer was passed, anid tlle urine found to be free of sugar.He was givenl 50 c.cm. of 25 per cenit.glucose initr aveniously.This led to rapid recovery, the patient changing in tive miniutes from a coi;dition of coma to complete consciousness and intelli- gese.Examissed half an hour later the plantar reflexes were equivocal, the definite extensor response beinig no longer obtained.During the niiglht of Dem ber 4tl the patient had a secoid 41tack of insulin cona.As before, this was heralded by shiverinig and a feeliing of coldniess.He was easily revived by rectal glucose followed by a drink of tea with sugar.Examnined onl December 14th, the knee-jerks were brisk, ankle- jerks slight, and the plantar responise was absent in both feet.No further attempt to lower the blood sugar to the accepted niormal value was deemed advisable at this stage, and the patienit was discharged on De-ember 18th with instrPictions to attend the out-patient departmenit.The dose of insulin was theni 15 units morning anid evening.This was increased later, anid when last examiied OnI February 6th, 1929, he was taking 25 units morning aIld evening; the blood sugar was 0.16 per cent., and the urine was sugar-free, CAss IT.Tlhe patient, a womnan aged 66, was admiitted to Ulniversity College Hospital on February 20th, 1929, suffering from severe diabetes.Emaciation was extreme, her weiglht beinig only 4! st., and the urine contained *a large amounit of sugar and acetonie bodies.The knee-jerks were absent and the planitar reflexes flexor.Shle was given a diet of 1,0W calories with 20 uniits of insulin twice daily.With this dosage of insulin the glycosuria persisted, and the blood sugar was 0.23 per cent.; in consequence tlhe dose was in- creased, together with the diet, until she was taking 100 units a day on a diet of 1,500 calories.The urine then became sugar-free.
Multiple Complications and Death Following Protriptyline Overdose
Authors: David J. Greenblatt
Journal: JAMA (1974)

MOST cases of fatal tricyclic antidepressant poisoning involve imipramine hydrochloride or amitriptyline hydrochloride. In the present case, protriptyline hydrochloride overdose produced serious intoxication with multiple complications and was ultimately fatal. <h3>Report of a Case</h3> A 28-year-old white man was brought to the emergency ward of the Massachusetts General Hospital in a delirious, agitated state, having ingested an unknown quantity of protriptyline (Vivactyl) several hours previously. In the three years before admission, the patient had been hospitalized several times for depressive illness. He had received electroconvulsive therapy and tricyclic antidepressants. Protriptyline had been prescribed shortly before the present admission by a physician at another hospital. On arrival, the patient was agitated and combative, with a blood pressure of 80/40 mm Hg and a rectal temperature of 41 C (106 F). Naloxone hydrochloride (0.8 mg) was given intravenously with no response. Shortly after the patient's arrival, repetitive grand mal epileptic seizure activity
WORDS TO THE WISE
Authors: G. Hutchison
Journal: BMJ (1995)

The vermiform appendix is shaped like a worm, the adjective being derived from the Latin vermis, a word that is also used to describe the long, thin, middle lobe of the cerebellum. Vermis crops up in odd places. In Middle English the idea of a worm encompassed all small things that crept, crawled, or wriggled; reptiles and insects were worms, too. Since small, wriggling creatures are often unpleasant to associate with, the word began to be used for any creature that seemed unpleasant, and by the 15th century we find marmosets being referred to as worms. A Latinate alternative, vermin, …
Rapid Dexamethasone Suppression Test for Cushing's Syndrome
Authors: Joseph R. Tucci
Journal: JAMA (1967)

The practical application of the overnight dexamethasone suppression test for preliminary screening for Cushing's syndrome can be extended by the utilization of a steroid-creatinine ratio calculated from urine collected in a five-hour period, ie, 7:00 AM to 12:00 noon. The use of the urine ratio obviates the necessity of a quantitatively accurate urine collection, and hence makes it a more feasible outpatient screening procedure. A ratio of less than 4 makes the diagnosis of Cushing's syndrome highly unlikely, whereas a ratio greater than 4 requires that further studies be performed to establish a definitive diagnosis of Cushing's syndrome.
Structure of murine Ia antigens. Two dimensional electrophoretic analyses and high pressure liquid chromatography tryptic peptide maps of products of the I-A and I-E subregions and of an associated invariant polypeptide.
Authors: M McMillan, J A Frelinger, P P Jones, D B Murphy, H O McDevitt, L Hood
Journal: The Journal of Experimental Medicine (1981)

We demonstrate that an invariant polypeptide, first described by Jones et al. (21), co-immunoprecipitates with our Ia molecules, that its interaction with Ia polypeptides varies with haplotype, and that it is not a precursor of the Aalpha, Abeta, Ealpha, or Ebeta. polypeptides. We also show that the polypeptides that we have previously characterized are contaminated with very little, if any, invariant protein. Further, we have used our high-pressure liquid chromatography tryptic peptide map technique to formally map the genes encoding Aalpha, Abeta, and Ebeta to the I-A subregion using recombinant and F1 hybrid mice.
To a Workshopped Poem
Authors: Jane Schapiro
Journal: JAMA (1992)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Antitobacco Project Begins
Authors: Ken Sternberg
Journal: JAMA (1988)

ABSTRACT ABOUT 54 000 INFORMATION kits offering reasons not to use tobacco will be distributed to the teachers of 1 million first grade students throughout the United States by the end of the year. By doing this, the American Heart and Lung associations and the American Cancer Society hope to successfully launch the Smoke-Free Class of 2000, a collaborative effort they believe will reduce the number of future smokers (JAMA 1988;260:13-14).The program, officially inaugurated Aug 30 in Washington, DC, seeks to foster a more health-conscious attitude among the next generation, says Tim Elsner, a spokesman for the Dallas-based heart association. The precise number of schools involved in the program has not yet been determined, but Elsner says that the three organizations are already involved in related classroom-based education projects, so the new program should be widely accepted. Local chapters have already paid for the information kits, so their staffs will
WHO targets environmental impact on child health
Authors: R. Watson
Journal: BMJ (2002)

A publication that looks at how the environment affects children's health will be released in Brussels on Monday (15 April), at the start of the European Commission's Green Week.

Children, it says, might be the “canaries in the coalmines”—the first people to show adverse health effects resulting from environmental factors, with possible lifelong implications for adults and children.

The 225 page document, a collaborative venture between the WHO's Regional Office for Europe and the European Environment Agency, is intended to promote a better understanding among scientists and professionals working in child health and environmental …
Microporation Is a Valuable Transfection Method for Gene Expression in Human Adipose Tissue–derived Stem Cells
Authors: Yan-Hsiung Wang, Mei-Ling Ho, Je-Ken Chang, Hai-Chang Chu, Shu-Chin Lai, Gwo-Jaw Wang
Journal: Molecular Therapy (2008)

Stem cells are a promising resource for gene therapy. Adipose tissue–derived stem cells (ADSCs) offer advantages because of their abundance and ease of isolation. However, it is difficult to transduce genes into ADSCs by common transfection methods, especially nonviral methods. We report here the use of a new electroporation method, termed "microporation," to transduce plasmids into human ADSCs (hADSCs). We determined optimal conditions that led to efficient transfection of >76.1% of the microporated hADSCs with only minimal cell damage or cytotoxicity. We demonstrated the expression of both enhanced green fluorescent protein (EGFP) and luciferase from different promoters in microporated hADSCs. More important, the microporated hADSCs retained their multipotency and reporter gene expression was maintained for >2 weeks in vitro and in vivo. We further showed that a Tet-ON-inducible gene expression system could be microporated into hADSCs and that this system was functional following transplantation of the microporated cells into nude mice. Taken together, our data demonstrate that microporation allows a highly efficient transfection of hADSCs, without impairing their stem cell properties. Stem cells are a promising resource for gene therapy. Adipose tissue–derived stem cells (ADSCs) offer advantages because of their abundance and ease of isolation. However, it is difficult to transduce genes into ADSCs by common transfection methods, especially nonviral methods. We report here the use of a new electroporation method, termed "microporation," to transduce plasmids into human ADSCs (hADSCs). We determined optimal conditions that led to efficient transfection of >76.1% of the microporated hADSCs with only minimal cell damage or cytotoxicity. We demonstrated the expression of both enhanced green fluorescent protein (EGFP) and luciferase from different promoters in microporated hADSCs. More important, the microporated hADSCs retained their multipotency and reporter gene expression was maintained for >2 weeks in vitro and in vivo. We further showed that a Tet-ON-inducible gene expression system could be microporated into hADSCs and that this system was functional following transplantation of the microporated cells into nude mice. Taken together, our data demonstrate that microporation allows a highly efficient transfection of hADSCs, without impairing their stem cell properties.
The Legacy of Well-Water Methemoglobinemia
Authors: John N. Lukens
Journal: JAMA (1987)

THE LANDMARK ARTICLE<sup>1</sup>reprinted in this issue ofThe Journalwas written by a pediatric resident, Hunter Comly, MD, who was encouraged by Robert Jackson, MD, to seek out the cause of cyanosis in two infants referred to Iowa City from the surrounding countryside. Central to the mystery's solution was a theory advanced by the father of the first patient that implicated well water in his daughter's recurrent episodes of cyanosis. Respecting Dr Jackson's "open minded attitude" toward the "cock and bull theory," the young Comly had water from the suspect well analyzed and in so doing demonstrated that well-water nitrates were a cause of methemoglobinemia in infants. A survey of the nitrate content of water from Iowa wells clearly demonstrated the public health dimension of the problem. Neither Comly nor Jackson pursued their interest in methemoglobinemia: Comly became a child psychiatrist, while Jackson distinguished himself as a pediatric
Evidence for the transmission of Salmonella from reptiles to children in Germany, July 2010 to October 2011
Authors: M Pees, W Rabsch, B Plenz, A Fruth, R Prager, S Simon, V Schmidt, S Münch, P G Braun
Journal: Eurosurveillance (2013)

This study examines the Salmonella status in reptiles kept in households with children suffering from gastroenteritis due to an exotic Salmonella serovar, to obtain information on possible transmission paths. A number of affected households (n=79) were contacted, and almost half (34/79) comprised at least one reptile in the home. Of the households, 19 were further studied, whereby a total of 36 reptiles were investigated. Samples were taken from the reptiles including the oral cavity, the cloaca, the skin and, in the case of lizards, the stomach, and isolation of Salmonella strains was performed using repeated enrichment and typing. Where the Salmonella serovars of the infected child and the reptile were identical, typing was followed by pulsed-field gel electrophoresis (PFGE). Bearded dragons (Pogona vitticeps) constituted 19 of 36 examined reptiles. Altogether 319 Salmonella isolates were investigated and 24 different serovars identified in the reptiles. In 15 of 19 households, an identical serovar to the human case was confirmed in at least one reptile (including 16 of all 19 bearded dragons examined). The results demonstrate that reptiles and especially bearded dragons shed various Salmonella serovars including those isolated from infected children in the respective households. Hygiene protocols and parents' education are therefore highly necessary to reduce the risk of transmission. From a terminological point of view, we propose to call such infections 'Reptile-Exotic-Pet-Associated-Salmonellosis' (REPAS).
Human major histocompatibility complex class II-restricted T cell responses in transgenic mice.
Authors: A Woods, H Y Chen, M E Trumbauer, A Sirotina, R Cummings, D M Zaller
Journal: The Journal of Experimental Medicine (1994)

Transgenic mice expressing human major histocompatibility complex (MHC) class II molecules would provide a valuable model system for studying human immunology. However, attempts to obtain human class II-restricted T cell responses in such transgenic mice have had only limited success, possibly due to an inability of mouse CD4 to interact efficiently with human MHC class II molecules. To circumvent this problem, we constructed recombinant MHC class II genes in which the peptide-binding domain was derived from human DR sequences whereas the CD4-binding domain was derived from mouse I-E sequences. Purified chimeric human/mouse MHC class II molecules were capable of specifically binding DR-restricted peptides. Human B cell transformants that expressed these chimeric MHC class II molecules could present peptide antigens to human T cell clones. Expression of these chimeric class II molecules in transgenic mice led to the intrathymic deletion of T cells expressing superantigen-reactive V beta gene segments, indicating that the chimeric class II molecules could influence the selection of the mouse T cell repertoire. These transgenic mice were fully capable of mounting human DR-restricted immune responses after challenge with peptide or whole protein antigens. Thus, the chimeric class II molecules can serve as functional antigen presentation molecules in vivo. In addition, transgenic mice expressing chimeric class II molecules could be used to generate antigen-specific mouse T cell hybridomas that were capable of interacting with human antigen-presenting cells.
Coexistence of Southeast Asian ovalocytosis and β‐thalassemia: A molecular and hematological analysis
Authors: Goonnapa Fucharoen, Supan Fucharoen, Sanita Singsanan, Kanokwan Sanchaisuriya
Journal: American Journal of Hematology (2006)

Abstract We describe hematological and molecular characterization of a Thai famale who had Southeast Asian ovalocytosis (SAO) associated with β + ‐thalassemia trait. The proband had mild microcytosis with Hb 12.9 g/dl, Hct 35.8%, MCV 74.4 fl, MCH 26.8 pg, MCHC 36.0 g/dl, and elevated Hb A 2 (5.6%), characteristics of β‐thalassemia trait. Peripheral blood film examination revealed prominent ovalocytosis. However, a one‐tube osmotic fragility (OF) test commonly used for thalassemia screening was negative and a normal OF curve was observed. Further polymerase chain reaction (PCR) analyses identified the β ‐28A‐G mutation in the β‐globin gene and a 27 bp deletion in erythrocyte band 3 protein gene, indicating a genetically compound heterozygote. Hematological data of the proband was comparatively presented with those of eight female and 15 male carriers of pure β‐thalassemia with the same mutation. The finding demonstrates that although the association of the SAO and β‐thalassemia does not produce a more severe clinical picture, this could lead to a mis‐screening of β‐thalassemia using an OF test as a primary screening test. Additional blood film examination followed by PCR could help in the detection of this unusual genetic interaction in the region. Am. J. Hematol., 2007. © 2006 Wiley‐Liss, Inc.