tbuckley/cabot-search huggingface dataset:
Abstract 4206: Identification of transcriptional regulatory motifs that control gene expression in premenopausal women with previous history of breast cancer
Authors: Oscar D. Cano, Yesim Gökmen-Polar, Goswami Chirayu, Lang Li, Usha Sirimalle, Xiaoping Gu, Harikrishna Nakshatri, Sunil Badve
Journal: Cancer Research (2012)

Abstract Background: Age is an important risk factor for Breast Cancer. Older women have a 50 times greater risk for breast cancer as compared to younger women (under the age of 30 years). Aging is associated with activation of specific transcription factor networks. We hypothesized that premature activation of these programs may predispose to cancer. The aims of this study were 1) to identify active transcription factor networks controlling the expression of specific cancer-related genes in the normal breast and 2) to analyze whether premature activation of this network is associated with neoplastic transformation. Methods: Fresh frozen normal breast tissue of women, under the age of 50 years, with and without history of breast cancer was obtained (ncancer=8, nnon cancer=4). Total RNA was extracted, cDNA annealed and Whole-genome DASL assay (Illumina Corp) was conducted to identify genes that correlated with cancer outcome in premenopausal women. Differential expression analysis was performed using Partek® software program to identify the up or down regulated genes in women with cancer compared to those without. Transcription factors affecting the regulation of genes in patients with cancer were predicted using MotifModeler program (Liu et al). The resulting data was confirmed using qPCR analysis. Results: Whole genome DASL analysis showed differential expression of 34 genes (p-value &amp;lt;0.01). MotifModeler analysis predicted 18 transcription factors as major regulators of gene expression in premenopausal women with previous history of breast cancer. JUNB and FOS were the most significantly altered in this comparison. qPCR pre-validated the over expression of JUNB and FOS in association with cancer development. Further validation studies in an independent cohort are ongoing using Formalin-fixed Paraffin-embedded (FFPE) samples. Conclusion: Gene expression analysis identified transcriptional features of cancer outcome. Differential expression of the JUNB and FOS family of proteins, which are part of AP-1 transcription factor complex, may be correlated with neoplastic transformation in premenopausal women. Breast Cancer Program of the IU Simon Cancer Center funded this project. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4206. doi:1538-7445.AM2012-4206
Treatment of Brain Ischemia after Cardiac Angiography.
Authors: Gary Wise
Journal: Annals of Internal Medicine (1972)

Meeting Abstracts1 May 1972Treatment of Brain Ischemia after Cardiac Angiography.Gary Wise, M.D., Richard Leighton, M.D., F.A.C.P., Mary E. Fontana, M.D., James Burkholder, M.D., John Robinson, M.D., Richard Lewis, M.D., F.A.C.P.Gary Wise, M.D., Richard Leighton, M.D., F.A.C.P., Mary E. Fontana, M.D., James Burkholder, M.D., John Robinson, M.D., Richard Lewis, M.D., F.A.C.P.Author, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-76-5-868_4 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptFive normotensive patients developed severe brain dysfunction during cardiac angiography. Four had angiography because of recurrent, severe angina pectoris, and one patient had valvular aortic stenosis. Hemiplegia and other focal neurologic signs developed after coronary arteriography and left ventricular angiography. Hypotension, cardiac arrhythmias, chest pain, ECG changes, or clinical evidence of heart failure did not occur. The neurologic deficit disappeared when the blood pressure was raised to 160 to 180 mm Hg systolic by an intravenous infusion of either levarterenol bitartrate or phenylephrine. During the immediate postarteriogram period the focal neurologic dysfunction returned whenever the blood pressure was allowed to... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: Gary Wise, M.D.; Richard Leighton, M.D., F.A.C.P.; Mary E. Fontana, M.D.; James Burkholder, M.D.; John Robinson, M.D.; Richard Lewis, M.D., F.A.C.P.Affiliations: Columbus, Ohio PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byScanning electron microscopy of surface irregularities and thrombogenesis of polyurethane and polyethylene coronary catheters.Percutaneous catheter emboli: Sources and preventionComplication rate of coronary arteriography. A review of 5250 cases studied by a percutaneous femoral technique.Percutaneous Transfemoral Coronary Arteriography; Prevention of Morbid ComplicationsSystemic Heparinization for Femoral Percutaneous Coronary Arteriography 1 May 1972Volume 76, Issue 5Page: 868-869KeywordsAngiographyBlood pressureBrainHeart failureHypotensionIschemiaNorepinephrinePhenylephrineStable coronary artery diseaseStenosis ePublished: 1 December 2008 Issue Published: 1 May 1972 PDF downloadLoading ...
The role of microstructured and interconnected pore channels in a collagen-based nerve guide on axonal regeneration in peripheral nerves
Authors: Ahmet Bozkurt, Franz Lassner, Dan O’Dey, Ronald Deumens, Arne Böcker, Tilman Schwendt, Christoph Janzen, Christoph V. Suschek, Rene Tolba, Eiji Kobayashi, Bernd Sellhaus, S. Tholl, Lizette Eummelen, Frank Schügner, Leon Olde Damink, Joachim Weis, Gary A. Brook, Norbert Pallua
Journal: Biomaterials (2011)
The Nitrous Oxide Test
Authors: ANDREW G. MORROW, RICHARD J. SANDERS, EUGENE BRAUNWALD
Journal: Circulation (1958)

The diagnosis of a left-to-right shunt generally depends upon the demonstration of significant differences in oxygen content among the venae cavae, the chambers of the right heart, and the pulmonary artery. The inconclusive or misleading results sometimes obtained with this method have stimulated interest in improved technics. The wide arteriovenous difference that normally exists during the inhalation of nitrous oxide has been studied in the development of a more accurate diagnostic approach. The superiority of the nitrous oxide test over the oxygen method is demonstrated.
Soft Clinical Research on an Inpatient Unit: Effect of Findings on Treatment
Authors: Howard E. Book
Journal: Psychotherapy and Psychosomatics (1986)

This paper describes the value of identifying and processing intuitive hunches and impressionistic ideas that arose amongst psychiatrist/clinicians on an inpatient unit. Through semistructured meetings, these hunches generated psychodynamic formulations and therapeutic approaches that were integrated into already existing models of treatment to yield a better understanding of, and a more tailored approach to, the borderline patient and his/her impact on staff.
Effect of tissue shrinkage on histological tumour-free margin after excision of basal cell carcinoma
Authors: C. Blasdale, F.G. Charlton, S.C. Weatherhead, P. Ormond, C.M. Lawrence
Journal: British Journal of Dermatology (2009)

Histology reports of skin tumour excisions frequently describe a histological margin significantly less than the planned surgical excision margin.A novel method of marking visible tumour margin was devised. This allowed us to evaluate the accuracy of tumour detection and to compare tissue contraction of the clinically normal perilesional skin with that of tumour tissue following excision and fixation.Forty-four well-defined basal cell carcinomas were excised from 42 patients. The visible tumour edge was marked by scoring with a blade around its circumference prior to excision. This allowed comparison of visible and true histological tumour margin. The excision margin was carefully measured from the scored line and the tumour excised. After tissue fixation and processing the histological dimensions of tumour and perilesional margin skin were compared with the pre-excision measurements.The tumour edge was accurately identified to within 1 mm in 67% of margins and was underestimated in only 4%. The whole specimen contracted by a mean of 14%. Skin containing tumour contracted by a mean of 11% but adjacent tumour-free skin in the same plane contracted by a mean of 19%. There was no significant effect of age and site on difference in percentage shrinkage between tumour and margin.We underestimated tumour extent at only 4% of margins. Tissue shrinkage was the most important factor affecting eventual histological margin. Our novel technique allowed us to demonstrate that this shrinkage is not uniform across the specimen, but is disproportionately high in the tumour-free margin. This suggests that previous estimates of margin shrinkage, based on whole-specimen contraction measurements, may have been erroneously low.
Emergency Psychiatry: Principles and Practice
Authors: Walter A. Brown
Journal: JAMA (2009)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Journals JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Effect of Regular Salmeterol Treatment on Albuterol-induced Bronchoprotection in Mild Asthma
Authors: DEBORAH H. YATES, MIIN WORSDELL, PETER J. BARNES
Journal: American Journal of Respiratory and Critical Care Medicine (1997)

There is increasing evidence for the development of tolerance to the bronchoprotective effects of inhaled beta 2-agonists against bronchoconstrictor stimuli in asthma. With short-acting beta 2-agonists, this is more readily demonstrable using indirectly acting agents such as adenosine monophosphate (AMP), which may act via mast cell degranulation, than using methacholine (MCh), implying more rapid mast cell than smooth muscle desensitization. Desensitization may be greater with the long-acting beta 2-agonist, salmeterol, given its greater duration of receptor occupancy. In a double-blind, placebo-controlled crossover study, we investigated the effect of regular salmeterol on the protection conferred by albuterol using MCh- and AMP-induced bronchoconstriction. Sixteen mild asthmatic subjects not using inhaled glucocorticoids were randomized to treatment for 2 wk with inhaled salmeterol (50 micrograms b.i.d. via diskhaler) or identical placebo. Provocative concentrations of MCh and AMP causing a 20% fall in FEV1 (PC20) were measured 15 min after 200 micrograms albuterol, both before and after treatment. Mean MCh PC20 after albuterol decreased significantly after 2 wk of salmeterol treatment (mean 2.2 mg/ml before to 1.1 +/- 1.2 mg/ml after) compared with placebo (2.9 +/- 1.3 mg/ml before to 2.6 +/- 1.3 mg/ml after; p < 0.05), but this fell just short of statistical significance when analyzed as change in doubling dilutions (1.1 +/- 0.4 versus 0.18 +/- 0.4; p = NS). Mean PC20 to AMP was not significantly affected (mean 27.5 +/- 1.5 mg/ml prior to salmeterol treatment and 9.5 +/- 1.5 mg/ml after treatment; p = NS compared with placebo). Thus, regular salmeterol treatment led to loss of bronchoprotection by albuterol to MCh but not to AMP challenge, implying an absence of mast cell beta 2-adrenoceptor downregulation with regular salmeterol therapy.
BCL6 controls neurogenesis through Sirt1-dependent epigenetic repression of selective Notch targets
Authors: Luca Tiberi, Jelle van den Ameele, Jordane Dimidschstein, Julie Piccirilli, David Gall, Adèle Herpoel, Angéline Bilheu, Jerome Bonnefont, Michelina Iacovino, Michael Kyba, Tristan Bouschet, Pierre Vanderhaeghen
Journal: Nature Neuroscience (2012)
Application of mass spectrometry for target identification and characterization
Authors: Joseph A. Loo, Dana E. DeJohn, Ping Du, Tracy I. Stevenson, Rachel R. Ogorzalek Loo
Journal: Medicinal Research Reviews (1999)

Mass spectrometry-based methodologies span the vast expanse of drug discovery. Both electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) support proteomics-based research projects by identifying proteins separated and isolated by polyacrylamide gel electrophoresis. MALDI-MS-based surface scanning of one-dimensional isoelectric focusing gels, "virtual 2-D gel electrophoresis," represents a potentially high throughput means to map proteins and to determine protein profiles. Mass spectrometry can also be used to directly study the covalent and noncovalent interactions of drug molecules and biomolecule targets. Drug binding examples discussed include the binding of covalent and noncovalent inhibitors to src SH2 domain protein, and the interaction of aminoglycoside antibiotic neomycin and HIV Tat peptide-TAR RNA. © 1999 John Wiley & Sons, Inc. Med Res Rev, 19, No. 4, 307–319, 1999.
Associations of blood levels of PCB, HCHS, and HCB with numbers of lymphocyte subpopulations, in vitro lymphocyte response, plasma cytokine levels, and immunoglobulin autoantibodies.
Authors: V Daniel, W Huber, K Bauer, C Suesal, C Conradt, G Opelz
Journal: Environmental Health Perspectives (2001)

Pentachlorophenol (PCP), hexachlorocyclohexane-[alpha], -beta, and -[gamma] (HCH-[alpha], -beta, and -[gamma]), polychlorinated biphenyls (PCBs), and hexachlorobenzene (HCB) are widely distributed industrial chemicals. They are suspected to induce immunologic impairments in exposed individuals. We examined dose-response relationships of blood levels of these chemicals with cellular (numbers of lymphocyte subpopulations, in vitro lymphocyte response) or humoral (plasma cytokine levels, immunoglobulin autoantibodies) immunologic dysfunctions. We studied 146 patients who had been occupationally exposed primarily to PCBs for more than 6 months. Lymphocyte subpopulations, in vitro responses to mitogens and allogeneic stimulator cells, plasma neopterin, cytokines, soluble cytokine receptors, soluble adhesion molecules, anti-Ig autoantibodies, and liver transaminases were determined. Blood levels of the different compounds were strongly correlated with one another. There were only weak dose-response relationships between blood levels of PCBs with cellular immune parameters, and of HCHs and HCB with humoral immune parameters. An exception was the statistically significant negative association of HCB with interferon-[gamma] (IFN-[gamma]), indicating that HCB has a significant impact on Th1 lymphocytes. Patients with HCB blood levels above the mean of 1,109 ng/L more often had undetectable IFN-[gamma] blood levels than patients below the mean. Patients with increased PCB 138 (> 710 ng/L) had more frequently undetectable interleukin-4 blood levels than patients with PCB 138 below the mean, and patients with increased PCB 101 (> 31 ng/L) more often had low DR+ cell counts in the blood (< 190/microL) than patients with PCB 101 below the mean. To assess possible cumulative effects, we compared patients who had blood levels of all compounds below background with patients who had blood levels of all compounds above background. Patients with low or absent blood levels of the compounds studied had higher IFN-[gamma] plasma levels, providing some evidence for a cumulative effect of several weakly active compounds. In conclusion, exposure to PCBs, HCB, or HCHs is associated with weak immunologic abnormalities. These results contrast with those obtained in earlier studies of blood levels of PCP, which showed a strong dose-dependent relationship with immunologic impairments. Our data suggest that long-term exposure of patients to HCB suppresses IFN-[gamma] production.
Oxygen deprivation-induced injury to isolated rabbit kidney tubules.
Authors: J M Weinberg
Journal: Journal of Clinical Investigation (1985)

The utility of freshly isolated suspensions of rabbit tubules enriched in proximal segments for studying the pathogenesis of oxygen deprivation-induced renal tubular cell injury was evaluated. Oxygenated control preparations exhibited very good stability of critical cell injury-related metabolic parameters including oxygen consumption, cell cation homeostasis, and adenine nucleotide metabolism for periods in excess of 2 h. Highly reproducible models of oxygen deprivation-induced injury and recovery were developed and alterations of injury-related metabolic parameters in these models were characterized in detail. When oxygen deprivation was produced under hypoxic conditions, tubules sustained widespread lethal cell injury and associated metabolic alterations within 15-30 min. However, when oxygen deprivation was produced under simulated ischemic conditions, tubules tolerated 30-60 min with only moderate amounts of lethal cell injury occurring, a situation similar to that seen with ischemia in vivo. Like ischemia in vivo, simulated ischemia in vitro was characterized by a fall in pH during oxygen deprivation. No such fall in pH occurred in the hypoxic model. To test whether this fall in pH could contribute to the protection seen during simulated ischemia in vitro, tubules were subjected to hypoxia at medium pHs ranging from 7.45 to 6.41. Striking protection from hypoxic injury was seen as pH was reduced with maximal protection occurring in tubules made hypoxic at pHs below 7.0. Measurements of injury-associated metabolic parameters suggested that the protective effect of reduced pH may be mediated by pH-induced alterations of tubule cell Ca++ metabolism. This study has, thus, defined and characterized in detail a new and extremely versatile model system for the study of oxygen deprivation-induced cell injury in the kidney and has established that pH alterations play a major role in modulating such injury.
Pharmacoepidemiology of Niacin
Authors: Paul W. Jungnickel
Journal: Annals of Internal Medicine (1995)

Letters1 March 1995Pharmacoepidemiology of NiacinPaul W. Jungnickel and Pierre A. MaloleyPaul W. JungnickelSearch for more papers by this author and Pierre A. MaloleySearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-122-5-199503010-00014 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:We feel that Gray and colleagues' data [1] do not support their claim that controlled-release and immediate-release niacin have similar toxic effects when used in equipotent doses. Because their study did not include a control group, it did not provide a basis for evaluating differences between the controlled-release and immediate-release forms. The study design probably also resulted in an underestimation of the incidence of niacin-associated hepatotoxicity (the most serious niacin toxicity). Hepatotoxicity was identified by notations on the medical record or by evidence of elevated alkaline phosphatase or aminotransferase levels. However, the authors did not report the ...References1. Gray DR, Morgan T, Chretien SD, Kashyap ML. Efficacy and safety of controlled-release niacin in dyslipoproteinemic veterans. Ann Intern Med. 1994; 121:252-8. Google Scholar2. McKenney JM, Proctor JD, Harris S, Chinchili VM. A comparison of the efficacy and toxic effects of sustained-vs immediate-release niacin in hypercholesterolemic patients. JAMA. 1994; 271:672-7. Google Scholar3. Rader JI, Calvert RJ. Hepatic toxicity of unmodified and time-release preparations of niacin. Am J Med. 1992; 92:77-81. Google Scholar4. Etchason JA, Miller TD, Squires RW, Allison TG, Gau GT, Martialla J, et al. Niacin-induced hepatitis: a potential side effect with low-dose time-release niacin. Mayo Clin Proc. 1991; 66:23-8. Google Scholar Author, Article, and Disclosure InformationAuthors: Paul W. Jungnickel; Pierre A. MaloleyAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 March 1995Volume 122, Issue 5Page: 392-393KeywordsAminotransferasesClinical trialsEnzymesLiverNiacinPharmacoepidemiologyPhosphatasesResearch laboratoriesSerum glutamic oxaloacetic transaminaseToxicity ePublished: 15 August 2000 Issue Published: 1 March 1995 Copyright & PermissionsCopyright © 1995 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Taking Stock of Volume-Outcome Studies
Authors: Colin B. Begg, Peter T. Scardino
Journal: Journal of Clinical Oncology (2003)

Article Tools EDITORIALS Article Tools OPTIONS & TOOLS Export Citation Track Citation Add To Favorites Rights & Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/JCO.2003.11.022 Journal of Clinical Oncology - published online before print September 21, 2016 PMID: 12560422 Taking Stock of Volume-Outcome Studies Colin B. BeggxColin B. BeggSearch for articles by this author , Peter T. ScardinoxPeter T. ScardinoSearch for articles by this author Show More Memorial Sloan-Kettering Cancer Center, New York, NY https://doi.org/10.1200/JCO.2003.11.022 Full Text PDF Figures and Tables "Taking Stock of Volume-Outcome Studies." Journal of Clinical Oncology, 21(3), pp. 393–394© 2003 by American Society of Clinical Oncology
Transrectal Ultrasound–Guided Implantation of Adjustable Continence Therapy (ProACT): Surgical Technique and Clinical Results After a Mean Follow-Up of 2 Years
Authors: Andrea Gregori, Ai Ling Romanò, Francesco Scieri, Francesco Pietrantuono, Giacomo Piero Incarbone, Antonio Salvaggio, Antonio Granata, Franco Gaboardi
Journal: European Urology (2009)