tbuckley/cabot-search huggingface dataset:
Abstract 1318: Mps1 is modified by sumoylation during the cell cycle
Authors: Agnese Restuccia, Feikun Yang, Yao Yixin, Wei Dai
Journal: Cancer Research (2014)

Abstract Mps1 is a dual specificity protein kinase with crucial roles in regulating the spindle assembly checkpoint and chromosome microtubule attachment. Molecular mechanisms that regulate Mps1 levels and its activity during mitosis remain unclear. Given that sumoylation plays an important role in mitotic progression, we investigated if Mps1 could be modified by SUMO conjugation. Endogenous sumoylated Mps1 was detected by nickel affinity pull-down from HeLa cells that constitutively expressed His6-SUMO1 or His6-SUMO2. When HEK293T cells transfected with a plasmid construct expressing Myc-Mps1, along with plasmids expressing His-SUMO-1 and FLAG-UBC9, were analyzed sumoylated Mps1 was also detected in both asynchronized and mitotic cell populations. Expression of wild-type FLAG-SENP1 completely abrogated sumoylated Mps1 compared with cells transfected with enzymatically defective FLAG-SENP1, confirming that Mps1 is modified by sumoylation. We also identified potential lysine residues of Mps1 that were sumoylated by the mutagenesis approach. Our study reveals that Mps1 is modified via sumoylation and that future experiments will be centered on understanding the biological function of this modification during the cell cycle. Citation Format: Agnese Restuccia, Feikun Yang, Yao Yixin, Wei Dai. Mps1 is modified by sumoylation during the cell cycle. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1318. doi:10.1158/1538-7445.AM2014-1318
A study of the cellular infiltrate of the proximal jejunal mucosa in ulcerative colitis and Crohn's disease.
Authors: R Ferguson, R N Allan, W T Cooke
Journal: Gut (1975)

The cellular infiltrate of the proximal jejunum has been compared between 20 subjects without gastrointestinal disease, 20 subjects with ulcerative colitis, and 26 subjects with Crohn9s disease. A significant increase (P smaller than 0.001) in the plasma cell infiltrate was noted in subjects with ulcerative colitis and a more marked increase in subjects with Crohn9s disease (P smaller than 0.001). Such increases may be relevant to raised serum IgA levels following panproctocolectomy for ulcerative colitis or recurrence in the small intestine in Crohn9s disease.
Radiation and Life
Authors: Jane M. Orient
Journal: JAMA (1986)

Although many pollutants of our civilization are new, radiation has been experienced by living organisms since their very beginning. Human activity—most significantly in medicine—has simply added to the dose. In a concise and lucid fashion, Hall explains the physics and the biologic effects of radiation, and its uses and dangers. Although written for the layperson, the book is also instructive for physicians, most of whom are unfamiliar with the radiation hazards faced by astronauts (and possibly by travelers on the Concorde), the indoor radon problem in energy-efficient homes, and the technique of positron emission tomography. The book is an antidote to the prevailing misinformation about the dangers of radiation. Hall continually emphasizes the need for perspective, ie, for balancing risks and benefits, and for comparing risks with the risks of practical alternatives (rather than with a utopian risk-free state). For example, Hall contrasts the relatively small public health problems resulting
Oral lichenoid reactions associated with amalgam: improvement after amalgam removal
Authors: A. Dunsche, I. Kastel, H. Terheyden, I.N.G. Springer, E. Christophers, J. Brasch
Journal: British Journal of Dermatology (2003)

The pathogenetic relationship between oral lichenoid reactions (OLR) and dental amalgam fillings is still a matter of controversy.To determine the diagnostic value of patch tests with amalgam and inorganic mercury (INM) and the effect of amalgam removal in OLR associated with amalgam fillings.In 134 consecutive patients 467 OLR were classified according to clinical criteria. One hundred and fifty-nine biopsies from OLR lesions were histologically diagnosed according to the World Health Organization criteria for oral lichen planus (OLP) and compared with 47 OLP lesions from edentulous patients without amalgam exposure. One hundred and nineteen patients were patch tested with an amalgam series. In 105 patients (357 of 467 lesions) the amalgam fillings were removed regardless of the patch test results and OLR were re-examined within a follow-up period of about 3 years. Twenty-nine patients refused amalgam removal and were taken as a control group.Eleven patients with OLR (8.2%) had skin lesions of lichen planus (LP). Histologically, the lesions in the OLR group could not be distinguished from those seen in the OLP group. Thirty-three patients (27.7%) showed a positive patch test to INM or amalgam. Amalgam removal led to benefit in 102 of 105 patients (97.1%), of whom 31 (29.5%) were cured completely. Of 357 lesions, 213 (59.7%) cleared after removal of amalgam, whereas 65 (18.2%) did not improve. In the control group without amalgam removal (n = 29) only two patients (6.9%) showed an improvement (P < 0.05). Amalgam removal had the strongest impact on lesions of the tongue compared with lesions at other sites (P < 0.05), but had very little impact on intraoral lesions in patients with cutaneous LP compared with patients without cutaneous lesions (P < 0.05). Patients with a positive patch test reaction to amalgam showed complete healing more frequently than the amalgam-negative group (P < 0.05). After an initial cure following amalgam removal, 13 lesions (3.6%) in eight patients (7.6%) recurred after a mean of 14.6 months.Of all patients with OLR associated with dental amalgam fillings, 97.1% benefited from amalgam removal regardless of patch test results with amalgam or INM. We suggest that the removal of amalgam fillings can be recommended in all patients with symptomatic OLR associated with amalgam fillings if no cutaneous LP is present.
A Placebo-Controlled Randomized Trial of Warfarin in Idiopathic Pulmonary Fibrosis
Authors: Imre Noth, Kevin J. Anstrom, Sara Bristol Calvert, Joao de Andrade, Kevin R. Flaherty, Craig Glazer, Robert J. Kaner, Mitchell A. Olman
Journal: American Journal of Respiratory and Critical Care Medicine (2012)

Animal and human studies support the importance of the coagulation cascade in pulmonary fibrosis.In a cohort of subjects with progressive idiopathic pulmonary fibrosis (IPF), we tested the hypothesis that treatment with warfarin at recognized therapeutic doses would reduce rates of mortality, hospitalization, and declines in FVC.This was a double-blind, randomized, placebo-controlled trial of warfarin targeting an international normalized ratio of 2.0 to 3.0 in patients with IPF. Subjects were randomized in a 1:1 ratio to warfarin or matching placebo for a planned treatment period of 48 weeks. International normalized ratios were monitored using encrypted home point-of-care devices that allowed blinding of study therapy.The primary outcome measure was the composite outcome of time to death, hospitalization (nonbleeding, nonelective), or a 10% or greater absolute decline in FVC. Due to a low probability of benefit and an increase in mortality observed in the subjects randomized to warfarin (14 warfarin versus 3 placebo deaths; P = 0.005) an independent Data and Safety Monitoring Board recommended stopping the study after 145 of the planned 256 subjects were enrolled (72 warfarin, 73 placebo). The mean follow-up was 28 weeks.This study did not show a benefit for warfarin in the treatment of patients with progressive IPF. Treatment with warfarin was associated with an increased risk of mortality in an IPF population who lacked other indications for anticoagulation.
Contribution of endothelium derived relaxing factors to acetylcholine induced vasodilatation in the rat kidney
Authors: F. Vargas, J M. Sabio, J. d. D. Luna
Journal: Cardiovascular Research (1994)

The aim was to evaluate the contribution of nitric oxide (NO) and endothelium derived hyperpolarising factor (EDHF) to the endothelium dependent, acetylcholine induced vasodilatation in isolated perfused rat kidney.The renal response to acetylcholine was compared in phenylephrine preconstricted renal vasculature under basal conditions and after the infusion of N omega-nitro-L-arginine (L-NAME), an inhibitor of NO synthesis, and tetraethylammonium, a non-specific blocker of potassium channels that inhibits acetylcholine induced hyperpolarisation. These inhibitors were given alone and together. In another experiment, the renal response to acetylcholine was compared when the vasculature was preconstricted with phenylephrine or with 40 and 80 mM KCl under basal conditions and after the infusion of L-NAME. All experiments were done in the presence of indomethacin.Inhibition of NO generation with L-NAME reduced the vasodilator responses to acetylcholine by approximately 50%, and enhanced the response to sodium nitroprusside in the isolated perfused kidney preconstricted with phenylephrine. Infusion of tetraethylammonium also decreased the response to acetylcholine by approximately 50% and increased vasodilatation responses to sodium nitroprusside. The simultaneous administration of both inhibitors (L-NAME and tetraethylammonium) had a summational effect which almost completely suppressed acetylcholine induced vasodilatation. Increasing concentrations of extracellular potassium produced a dose related decrease in acetylcholine induced vasodilatation. These attenuated responses were almost abolished after the infusion of L-NAME.Our results suggest that the vasodilator response to acetylcholine in isolated perfused rat kidneys is subserved by EDHF and nitric oxide, both endothelium derived mediators participating to a similar extent.
Erythropoietic precursors in mice with phenylhydrazine‐induced anemia
Authors: Hiroshi Hara, Makio Ogawa
Journal: American Journal of Hematology (1976)

Abstract Using a methylcellulose cell culture technique, we studied the serial changes in erythropoietic precursors in the femur, spleen, and blood of mice under erythropoietic stimuli. Phenylhydrazine hydrochloride, in the dosage of 60 mg/kg, was injected into mice subcutaneously on days 0, 1, and 3, and mice were sacrificed on days 0, 2, 4, 7, and 10 for assessment of erythropoietic precursors. Significant changes were observed for all hemopoietic organs in the number of erythrocytic burst‐forming units (BFU‐E) and erythrocytic colony‐forming units (CFU‐E). Only BFU‐E were present in blood, and their maximal increase was noted on day 2. While marrow BFU‐E continuously decreased, maximal increase of CFU‐E noted on day 4. Splenic BFU‐E and CFU‐E increased until day 4 and declined subsequently. These observations suggest the presence of significant migration of BFU‐E in mice under erythropoietic stimuli and stress the importance of studies on all hemopoietic organs in the assessment of murine hemopoiesis.
Clostridial species in the pathogenesis of necrotizing enterocolitis in patients with neutropenia
Authors: D. Gregg Hopkins, James P. Kushner
Journal: American Journal of Hematology (1983)

Abstract The role of gram‐negative bacteria in the pathogenesis of necrotizing enterocolitis has been stressed. We describe a 21‐year‐old woman with chronic neutropenia with cyclic fluctuations who died of necrotizing enterocolitis due to Clostridium septicum. Seven patients with cyclic neutropenia and necrotizing enterocolitis have been described in the literature and in each case in which bacteriologic data were reported, a Clostridial infection was documented. Patients with cyclic neutropenia should be treated with antibiotics effective against Clostridial species when abdominal symptoms complicate neutropenic periods.
Profound hypophosphataemia in patients collapsing after a "fun run".
Authors: G Dale, J A Fleetwood, J S Inkster, J R Sainsbury
Journal: BMJ (1986)

Health of juvenile delinquentsIntermediate treatment centres were established in 1969 to enable delin- quent adolescents to remain within their communities under supervision, avoiding the imposition of a custodial sentence.'This study compares the physical health of a group of delinquent boys who attended Parkway, an intermediate treatment centre in a deprived inner city area, with that of non- delinquents. Subjects, methods and resultsTwenty four delinquents admitted during one year were eligible for the study.They were matched with 24 boys who were at the same school and in the same year and who lived in the same postal district.A medical history was obtained, general physical examination was performed, and hearing and vision were assessed.Student's t and the X2 test were used as appropriate.The table shows the physical and social characteristics of the boys.There was no difference in birth weight, the incidence of perinatal problems, or the number ofhospital admissions between the two groups.Urinalysis showed normal results in all cases.Smoking was more common among delinquents (p<0-01).The Physical and social characteristics ofdelinquent and non-delinquent boys. (Figures are means (SD))
Control of systemic B cell-mediated autoimmune disease by nonmyeloablative conditioning and major histocompatibility complex-mismatched allogeneic bone marrow transplantation
Authors: Roelof Flierman, Hendrik J. Witteveen, Ellen I. H. van der Voort, Tom W. J. Huizinga, René R. P. de Vries, Willem E. Fibbe, René E. M. Toes, Jacob M. van Laar
Journal: Blood (2004)
TP53, SF3B1, and NOTCH1 mutations and outcome of allotransplantation for chronic lymphocytic leukemia: six-year follow-up of the GCLLSG CLL3X trial
Authors: Peter Dreger, Andrea Schnaiter, Thorsten Zenz, Sebastian Böttcher, Marianna Rossi, Peter Paschka, Andreas Bühler, Sascha Dietrich, Raymonde Busch, Matthias Ritgen, Donald Bunjes, Matthias Zeis, Michael Stadler, Lutz Uharek, Christof Scheid, Ute Hegenbart, Michael Hallek, Michael Kneba, Norbert Schmitz, Hartmut Döhner, Stephan Stilgenbauer
Journal: Blood (2013)
Effect of penicillamine treatment on immune complexes in two cases of seropositive juvenile rheumatoid arthritis
Authors: F. P. Bresnihan, B. M. Ansell
Journal: Annals of the Rheumatic Diseases (1976)

<b>Bresnihan, F. P., and Ansell, B. M. (1976).</b><i>Annals of the Rheumatic Diseases,</i><b>35</b>, 463-465. <b>Effect of penicillamine treatment on immune complexes in two cases of seropositive juvenile rheumatoid arthritis.</b> A correlation has previously been observed between the presence of enhancing complexes and cutaneous vasculitis in rheumatoid arthritis. Two patients with seropositive juvenile rheumatoid arthritis are described in whom enhancing complexes were detected before the appearance of cutaneous vasculitis. Their contrasting response to penicillamine is discussed in relation to the role of rheumatoid factor and antinuclear antibodies.
Housing conditions and ill health.
Authors: C J Martin, S D Platt, S M Hunt
Journal: BMJ (1987)

Lack of empirical evidence that living in damp houses has detrimental effects on health may partly be due to inadequate research. A preliminary study was therefore carried out of a random sample of council owned residences in a deprived area of Edinburgh, a respondent from consenting households being interviewed to obtain a profile of the physical and mental health of all adults and children. In addition, information was gathered about other factors that might be important, particularly smoking and selective bias in the allocation of tenants to houses. Independent measures of dampness were made by environmental health officers. No conclusive effects of damp on the health of adults were identified. Nevertheless, children living in damp houses, especially where fungal mould was present, had higher rates of respiratory symptoms, which were unrelated to smoking in the household, and higher rates of symptoms of infection and stress. Housing should remain an important public health issue, and the effects of damp warrant further investigation.
Mast Cells and Scleroderma
Authors: Pascal Chanez
Journal: JAMA (1990)

To the Editor.—  We read with great interest the article by Dr Claman 1 on the putative importance of mast cells in the integrated view of the pathogenesis of scleroderma. He noted increased numbers of mast cells in the skin of patients with active scleroderma and related this finding to skin fibrosis. Many patients with scleroderma experience lung involvement, usually characterized by fibrosing alveolitis with variable inflammation. 2 Therefore, it may be expected that mast cells would be increased in the lungs of those patients with scleroderma who have interstitial inflammation, since mast cells may be increased in lung fibrosis 3 and there is an increase in mast cells in the skin of patients with scleroderma.  Study. —  We studied 8 patients (40 to 63 years old) who had been suffering from skin scleroderma for the previous 15 to 29 years. Four of these patients had an interstitial lung disease
Seriously deficient professional performance
Authors: S. Brearley
Journal: BMJ (1996)

The Medical (Professional Performance) Act, which became law last November, has given powers to the General Medical Council (GMC) to take action when it seems that a doctor's professional performance may be seriously deficient. The council has been concerned for some time about its inability to intervene when consistently poor care seems to result not from misconduct but from a doctor's inadequate knowledge and skills, and it sees the new powers as essential if it is to fulfil its duty to protect the public. There has also been external pressure for a means of addressing poor performance among doctors, and if the task had not been given to the GMC an alternative mechanism would almost certainly have been demanded.

Consultations with medical organisations, patients' representatives, and politicians before enactment of the legislation showed wide support for the proposed measure, but doctors are clearly worried that the resulting “performance procedures” could prove inquisitorial and oppressive.1 The GMC now has the task of devising assessment procedures that are reliable, fair, and enjoy public confidence but avoid being cumbersome or onerous. This is a complex and difficult task, given the wide variation in types of medical work and the very different skills …
Laparoscopic Findings in Two Adults with Chickenpox
Authors: R. Uribarrena, F. Garcíia-Bragado, C. Jiménez, C. Pérez, A. Rivero-Puente
Journal: Endoscopy (1988)

We report the laparoscopic findings in two previously healthy adults with chickenpox and hepatic involvement. Both patients were admitted to hospital with severe upper right quadrant abdominal pain coinciding with the skin rash. Liver function tests were abnormal. In case 1, round lesions with a necrotic center, 2 mm in diameter, in both hepatic lobes were observed. Morphologically they resembled the rash on the skin. Numerous granular lesions were also demonstrated. In case 2, laparoscopic examination was performed recently when the cutaneous rash had faded. Granular images were scarce; there was enlargement of the liver capsule with small depressed areas of pink color. These lesions were considered similar to those found in case 1, although in a more advanced stage of scarring and resolution. Both patients made a good recovery.
Wilfrid Gerald Harding
Authors: W. Harding, T. Harding
Journal: BMJ (2010)

Born in Berlin as Wilfrid Hoffmann, Wilfrid Gerald Harding came to Britain in 1933 and studied medicine at University College and University College Hospital Medical School. His studies were hampered by being interned as an enemy alien for many months, after the outbreak of the second world war. In 1943 he joined the British army as a medical officer while still a German national (two of his brothers fought for the German …
P235Viral-delivered gene therapy for treatment of CPVT
Authors: E Kurtzwald-Josefson, E Hochauser, S Khun, A Shainberg, D Aravot, M Eldar, M Arad
Journal: Cardiovascular Research (2014)

Catecholaminergic polymorphic ventriculartachycardia (CPVT) is a lethal human arrhythmia provoked by exercise or emotional stress. It is mediated by abnormal calcium release from the sarcoplasmic reticulum through "leaky" ryanodine channels. Beta-adrenergic blockers are the therapy of choice for human CPVT but they fail to achieve complete arrhythmia control in some of the cases. Gene therapy is a potential treatment for genetic and other diseases associated with inadequate expression of a key protein.We established a new gene-delivery system to target CASQ2 knock-out mice suffering from CPVT2. CASQ2 gene was cloned into pAAV-IRES-hrGFP plasmid. AAV9 recombinant vectors were generated by co-transfecting the expression plasmid into the AAV-293 cells with pHelper (carrying adenovirus-derived genes) and pAAV-RC (carrying AAV-9 replication and capsid genes), which express the transacting factors required for AAV replication and packaging in the AAV-293 cells. Viral particles were purified from crude cell lysates, concentrated and injected into the left ventricle of 12-14-week-old mice. Mice underwent provocation testing for arrhythima 7 weeks post viral infection. Cryosections staining demonstrated that cardiac muscle and lung tissues were CASQ2 and GFP positive while liver and spleen were GFP negative in the infected mice. Calsequestrin protein expression was elevated in the hearts of infected CASQ2 knock-out mice (n=10) compared to controls. A dose-dependent reduction in abnormal ventricular beats was observed in mice that expressed AAV9-delivered myocardial CASQ2 protein (R2=0.723, p<0.005).Viral gene-delivery eliminated sustained ventricular tachycardia in all infected mice (p=0.012) while more than 33% of the normal protein level was required to prevent non-sustained VT (n=5, p=0.003). In conclusion, we assembled a vector useful for cardiac gene delivery, thus creating a platform for gene therapy studies in inherited heart diseases. In vivo viral delivery of CASQ2 cDNA attenuated ventricular arrythmia in mice with CPVT2.
Female-to-Male Transmission of Human Immunodeficiency Virus
Authors: Nancy S. Padian
Journal: JAMA (1991)

--To examine rates of heterosexual transmission of human immunodeficiency virus (HIV) and associated risk factors and to determine the relative efficiency of female-to-male and male-to-female transmission.--Survey of infected individuals and their heterosexual partners recruited since 1985.--Participants were recruited from various HIV counseling and testing sites throughout California but were generally interviewed and tested in their homes.--Data from 379 couples at entry to the study are reported: 72 male partners of infected women and 307 female partners of infected men. The infected index case had a well-established source of risk; couples were eliminated if the direction of transmission could not be established. The majority of couples were monogamous since 1978, white, and in their 30s. Most partners did not know their serostatus at entry into the study.--HIV serostatus in the exposed sexual partner.--We observed one probable instance (1%) of female-to-male transmission compared with 20% transmission rates in the female partners of infected men. All couples were sampled in the same way. Male index cases were more likely to be symptomatic than female index cases.--The odds of male-to-female transmission were significantly greater than female-to-male transmission. The one case of female-to-male transmission was unique in that the couple reported numerous unprotected sexual contacts and noted several instances of vaginal and penile bleeding during intercourse.