tbuckley/cabot-search huggingface dataset:
Isolation and characterization of lipid microdomains from apical and basolateral plasma membranes of rat hepatocytes
Authors: Amelia Mazzone, Pamela Tietz, John Jefferson, Richard Pagano, Nicholas F. LaRusso
Journal: Hepatology (2006)

Canalicular bile is formed by the osmotic filtration of water in response to osmotic gradients generated by active transport at the apical and basolateral plasma membrane domains of hepatocytes. We recently demonstrated that mixed plasma membrane fractions isolated from rat hepatocyte couplets contain lipid microdomains ("rafts") enriched in cholesterol and sphingolipids and AQP8 and 9. We isolated lipid microdomains from hepatocyte apical and basolateral plasma membrane domains using Triton X-100 as detergent, and characterized their lipid and protein composition. A Triton-insoluble band ("raft fraction") at the 5%/30% sucrose interface in both apical and basolateral fractions was enriched for alkaline phosphatase (apical) and Na/K ATPase (basolateral) and was negative for amino peptidase-N. This detergent-insoluble band was also positive for caveolin-1 (a "raft" associated protein) and negative for clathrin (a "raft" negative protein). Lipid analysis showed that, the Triton-insoluble fraction was highly enriched in cholesterol and sphingolipids. Immunofluorescence staining on hepatocyte couplets for both caveolin-1 and cholera toxin B showed a punctate distribution on both the apical and basolateral plasma membranes, consistent with localized membrane microdomains. Dot blot analysis showed that the "raft" associated ganglioside GM1 was enriched in the detergent-insoluble fraction both domains. Furthermore, exposure of isolated hepatocytes to glucagon, a choleretic agonist, significantly increased the expression of AQP8 associated with the apical microdomain fractions but had no effect on AQP9 expression in the basolateral microdomain fractions. In conclusion, "rafts" represent target microdomains for exocytic insertion and retrieval of "flux proteins", including AQPs, involved in canalicular bile secretion.
Keeping Crohn's Disease Quiet
Authors: Humphrey J. Hodgson
Journal: New England Journal of Medicine (1996)

Fifteen years ago, after the National Cooperative Crohn's Disease Study (NCCDS)1 had found that patients in remission who were given placebo fared better than those given corticosteroids, azathioprine, or a 5-aminosalicylate–sulfonamide compound, the approach to preventing relapse in patients with Crohn's disease was nihilistic. Now, the view among physicians is very different. New 5-aminosalicylates delivering larger amounts of the drug to the bowel can delay relapse,2 as can low-dose corticosteroids,3 and new, poorly absorbed corticosteroids, which have minimal side effects, are becoming available. Immunosuppressant drugs, at least in combination with a corticosteroid, have been proved to prolong corticosteroid-induced remissions.4 These . . .
Lead exposure at an early age substantially increases lead retention in the rat.
Authors: S Han, X Qiao, F W Kemp, J D Bogden
Journal: Environmental Health Perspectives (1997)

It has been hypothesized that the high rate of bone remodeling during childhood and the consequent high calcium and lead turnover result in a substantial reduction in bone lead stores so that much of the lead incorporated in bone during childhood does not persist into adulthood. We studied the effect of age at lead exposure on blood and organ concentrations of lead, calcium, and zinc 1-5 months after termination of lead ingestion. Blood and organ lead concentrations and contents 4 weeks after lead exposure ceased were significantly higher in the rats exposed beginning at 5 weeks of age than in those exposed beginning at 10 or 15 weeks old. Bone lead declined as the time since exposure increased. Despite this trend, the rats exposed when youngest had bone lead concentrations at 20 weeks after the termination of lead exposure that were higher than those of the other rats only 4 weeks after cessation of lead ingestion. Multiple regression analysis demonstrated that age at lead exposure remained a significant predictor of blood and organ lead concentrations and contents even after the inclusion of total lead consumed, body weight, and age at organ harvesting in the regression analysis. There were only small differences in organ calcium and zinc concentrations among treatment groups except for kidney calcium. The results do not support the hypothesis of rapid depletion of bone lead stores in young animals, but rather suggest that younger age at lead exposure is associated with greater lead retention and toxicity even in the absence of continued lead exposure.
Misuse of hypnosis.
Authors: H B Gibson
Journal: BMJ (1978)

given that of five patients with deteriorating renal function, three were on gentamicin alone and two on gentamicin plus a cephalosporin.Of the three on gentamicin alone they say, "All three patients were also suffering from transplant rejection and toxaemia, and interpretation of these results is therefore difficult."Of the two on gentamicin plus a cephalosporin they say, "Although in both cases rejection was occurring, we could not exclude nephrotoxicity."They do not present any evidence to explain why in the one situation they conclude, "attributable nephrotoxicity not observed," while in the other they conclude, "may have produced nephrotoxicity."Furthermore, in view of the accumulating evidence that gentamicin appears to be potentially more nephrotoxic than some other aminoglycosidesl-3 attention might have been drawn to the fact that, whether or not it was attributable, all five patients who showed deteriorating renal function were actually receiving gentamicin and not one of the other aminoglycosides included in the study.
A PAPER THAT CHANGED MY PRACTICE
Authors: D E Morris
Journal: BMJ (1995)

I do not think that the paper I want to praise was intended by its author to be erudite or particularly original. It was, however, the trigger for an important change that has benefited me and my patients.

Brian Goss argued that most out of hours consulting in general practice can more appropriately be carried out on medical premises than by home visits.1 Dr Goss illustrated his thesis by saying that he was writing the piece on the evening of a Saturday on call. “I could,” he wrote, “have done 30 visits today, but short surgeries at 9 00 …
Is AFP increase accompanyng HBeAg/antiHBe seroconversion in cirrhosis due to a special hepatocyte phenotype synthesizing and secreting AFP?
Authors: P. Fabris, D. Infantolino, M. Chiramonte, R. Barbazza, S. Fagiuoli, A. Floreani, R. Naccarato
Journal: Journal of Hepatology (1990)
RNASEL Arg462Gln variant is implicated in up to 13% of prostate cancer cases
Authors: Graham Casey, Phillippa J. Neville, Sarah J. Plummer, Ying Xiang, Lisa M. Krumroy, Eric A. Klein, William J. Catalona, Nina Nupponen, John D. Carpten, Jeffrey M. Trent, Robert H. Silverman, John S. Witte
Journal: Nature Genetics (2002)
556 COMPARISON OF DIAGNOSTIC CAPABILITY OF CONTRAST-ENHANCED CT, SONAZOID CONTRAST-ENHANCED US, GD-EOB-DTPA MRI AND CT ARTERIOPORTAL ANGIOGRAPHY IN DETECTING HISTOLOGICALLY PROVEN HCC NODULES SMALLER THAN 2CM
Authors: S.R. Kim, K. Mita, Y. Maekawa, M. Kudo, S. Imoto, T. Nakajima, K. Ando, K. Fukuda, T. Matsuoka, Y. Hayashi
Journal: Journal of Hepatology (2010)
New Tools Aid Violence Risk Assessment
Authors: Lynne Lamberg
Journal: JAMA (2007)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Planning Parental Leave
Authors: William D. Odell, H. James Williams, Barry M. Stults
Journal: Annals of Internal Medicine (1990)

Letters and Corrections1 April 1990Planning Parental LeaveWilliam D. Odell, MD, PhD, H. James Williams, MD, Barry M. Stults, MDWilliam D. Odell, MD, PhDSearch for more papers by this author, H. James Williams, MDSearch for more papers by this author, Barry M. Stults, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-112-7-553_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the Editor:The American College of Physicians position paper on parental leave for residents (15 December 1989) (1) appropriately calls attention to the desirability of residency programs having stated policies on this issue. At the University of Utah, we have had such policies for several years and have altered postdelivery schedules, switching mothers to less stressful and lighter rotations. Although we agree with the discussion and direction of the position paper, it omits another side of the story. The resident physician historically has not been identified as an employee or worker, but as a student participating in a learning...Reference1. . Parental leave for residents. Ann Intern Med. 1989;111:1035-8. LinkGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByConflict With Physician Pregnancy RevisitedParenting during ResidencyKwan Kew Lai, MD 1 April 1990Volume 112, Issue 7Page: 553-553KeywordsResidency Issue Published: 1 April 1990 PDF DownloadLoading ...
Effect of Chemokine Receptors CXCR4 and CCR7 on the Metastatic Behavior of Human Colorectal Cancer
Authors: Carl C. Schimanski, Stefan Schwald, Nektaria Simiantonaki, Caren Jayasinghe, Ursula Gönner, Vanessa Wilsberg, Theodor Junginger, Martin R. Berger, Peter R. Galle, Markus Moehler
Journal: Clinical Cancer Research (2005)

Abstract Purpose: The expression of chemokine receptors CXCR4 and CCR7 has been associated with tumor dissemination and poor prognosis in a limited number of tumor entities. However, no data are currently available on the impact of chemokine receptor expression on disease progression and prognosis in human colorectal cancer. Experimental Design: The expression of CXCR4 and CCR7 was evaluated in 96 patients with histologically confirmed colorectal cancers and in four colorectal cancer cell lines by immunohistochemical staining. Furthermore, cell migration assays were done with SW480, SW620, and LS174T cancer cells to confirm the effect of the CXCR4 ligand stromal cell–derived factor 1α on migration. Results: Human colorectal cancer specimens and cell lines displayed a CXCR4 and CCR7 expression with variable intensities. Interestingly, strong expression of CXCR4, but not of CCR7, was significantly associated with higher Union International Contre Cancer stages 3/4 (P = 0.0017), lymph node metastasis (P = 0.00375), and distant metastasis (P = 0.00003) and further correlated with a reduced 3-year survival rate (P = 0.1). Strong CXCR4 and CCR7 expression positively correlated with the location of the primary tumor in the rectum (P &amp;lt; 0.01). Furthermore, activation of CXCR4-expressing cancer cells by stromal cell–derived factor 1α resulted in a significant increase of cell migration (P &amp;lt; 0.014). Conclusion: Strong expression of CXCR4 by colorectal cancer cells is significantly associated with lymphatic and distant dissemination in patients with colorectal cancer as well as with cancer cell migration in vitro.
A novel interplay between Epac/Rap1 and mitogen-activated protein kinase kinase 5/extracellular signal-regulated kinase 5 (MEK5/ERK5) regulates thrombospondin to control angiogenesis
Authors: Robert C. Doebele, Frank T. Schulze-Hoepfner, Jia Hong, Alexandre Chlenski, Benjamin D. Zeitlin, Kushboo Goel, Suzana Gomes, Yuru Liu, Mark K. Abe, Jacques E. Nor, Mark W. Lingen, Marsha Rich Rosner
Journal: Blood (2009)
Pneumococcal Vaccine-Reply
Authors: Benjamin A. Lipsky
Journal: JAMA (1982)

In Reply.—  The demonstration that pneumococcal vaccine protects young, healthy people in unique high-risk settings, such as South African gold mines, hardly justifies licensing the product specifically for use in the elderly or the chronically ill, a population comprising more than 54 million Americans. For this group there is as yet no evidence that the vaccine prevents pneumococcal disease. 1,2 Among the 26 studies that the Bureau of Biologics used to evaluate the pneumococcal vaccine, only eight tested its efficacy; only one of these, in which 77 children with sickle cell anemia were immunized, demonstrated protection against pneumococcal infections for any of the targeted American populations. Since no responsible authority has recommended pneumococcal vaccination for the entire American public, it seems only reasonable, before licensing the vaccine, to demand evidence of its efficacy in the groups for whom it is intended.  The vaccine's approval by the FDA initiated a predictable
ESWL-assisted removal of a calcified pancreatic stent
Authors: V. Bove, A. Tringali, I. Boškoski, G. Gigante, G. Costamagna
Journal: Endoscopy (2013)

A 31-year-old man with calcified chronic pancreatitis underwent extracorporeal shock wave lithotripsy (ESWL) and endoscopic retrograde cholangiopancreatography (ERCP) with pancreatic sphincterotomy and extraction of stones in 2002. A pancreatic stent was inserted because of the presence of pancreatic strictures in the head and body of the pancreas (Cremer type III) [1]. The stent was inserted through the minor papilla owing to a dominant dorsal duct anatomy. The patient experienced symptom relief and underwent symptomatic stent exchange. The last stent exchange was performed in November 2009, when a 9-cm-long, 11.5-French plastic stent was inserted.
Excretion of Alcohol in the Urine as a Guide to Alcoholic Intoxication
Authors: H. W. Southgate, G. Carter
Journal: BMJ (1926)

<h3>Abstract</h3> Long-term memory is sensitive to both intrinsic and extrinsic motivation, but little is known about the relative influence of these two sources of motivation on memory performance across the adult lifespan. The study examined the effects of extrinsic motivation, manipulated via monetary reward, and curiosity, a form of intrinsic motivation, on long-term memory in healthy younger and older adults. During the incidental encoding phase on Day 1, 60 younger and 53 older participants viewed high- and low-curiosity trivia items as well as unrelated face stimuli. Half of the participants in each age group received financial rewards for correctly guessing trivia answers. On Day 2, participants completed a trivia recall test and an old-new recognition test for the face stimuli. Both curiosity and reward were associated with enhanced trivia recall, but the effects were interactive, such that only low-curiosity items benefitted from monetary reward. Neither curiosity nor reward affected face recognition performance in either age group. These findings indicate that individual and joint effects of intrinsic and extrinsic motivation on long-term memory are relatively preserved in healthy aging, a finding that highlights the viability of motivational strategies for memory enhancement into old age. Identifying conditions under which memory for unrelated information benefits from motivational spillover effects in younger and older adults is a priority for future research.
Bericht über den Verlauf einer psychotherapeutischen Behandlung bei einer an Katatonie leidenden Patientin
Authors: W.Th. Winkler
Journal: Psychotherapy and Psychosomatics (1957)

Research Articles| February 10 2010 Bericht über den Verlauf einer psychotherapeutischen Behandlung bei einer an Katatonie leidenden Patientin Subject Area: Psychiatry and Psychology W.Th. Winkler W.Th. Winkler Tübingen Search for other works by this author on: This Site PubMed Google Scholar Acta Psychotherapeutica et Psychosomatica (1957) 5 (2-4): 162–195. https://doi.org/10.1159/000285287 Article history Published Online: February 10 2010 Content Tools Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation W.Th. Winkler; Bericht über den Verlauf einer psychotherapeutischen Behandlung bei einer an Katatonie leidenden Patientin. Acta Psychotherapeutica et Psychosomatica 31 December 1957; 5 (2-4): 162–195. https://doi.org/10.1159/000285287 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsActa Psychotherapeutica et Psychosomatica Search Advanced Search Article PDF first page preview Close Modal This content is only available via PDF. 1957Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. You do not currently have access to this content.
The SHEDS-Wood Model: Incorporation of Observational Data to Estimate Exposure to Arsenic for Children Playing on CCA-Treated Wood Structures
Authors: Leila M. Barraj, Joyce S. Tsuji, Carolyn G. Scrafford
Journal: Environmental Health Perspectives (2007)

Lumber treated with chromated copper arsenate (CCA) compounds has been used in residential outdoor wood structures and public playgrounds. The U.S. Environmental Protection Agency (EPA) has conducted a probabilistic assessment of children's exposure to arsenic using the Stochastic Human Exposure and Dose Simulation model for the wood preservative scenario (SHEDS-Wood). The assessment relied on data derived from an experimental study conducted using adult volunteers and designed to result in maximum hand and wipe loadings to estimate the residue-skin transfer efficiency. Recent analyses of arsenic hand-loading data generated by studies of children actively involved in playing on CCA-treated structures indicate that the transfer efficiency coefficient and hand-loading estimates derived from the experimental study significantly overestimate the amount that occurs during actual play.Our goal was to assess the feasibility of using child hand-loading data in the SHEDS-Wood model and their impact on exposure estimates.We used data generated by the larger of the studies of children in SHEDS-Wood, instead of the distributions used by U.S. EPA. We compared our estimates of the lifetime average daily dose (LADD) and average daily dose (ADD) with those derived by the U.S. EPA.Our analysis indicates that data from observational studies of children can be used in SHEDS-Wood. Our estimates of the mean (and 95th percentile) LADD and ADD were 27% (10%) and 29% (15%) of the estimates derived by U.S. EPA.We recommend that the SHEDS-Woods model use data from studies of children actively playing on playsets to more accurately estimate children's actual exposures to CCA.
Dual Hormonal Replacement With Insulin and Recombinant Human Insulin-Like Growth Factor I in IDDM: Effects on glycemic control, IGF-I levels, and safety profile
Authors: Teresa Quattrin, Kathryn Thrailkill, Lester Baker, Jean Litton, Karen Dwigun, Melissa Rearson, Mary Poppenheimer, David Giltinan, Neil Gesundheit, Paul Martha, None Jr
Journal: Diabetes Care (1997)

To examine if dual replacement with insulin and rhIGF-I, recombinant human insulin-like growth factor I (rhIGF-I) may be safe and result in improved metabolic control and reduced insulin usage.Forty-three patients with IDDM were randomized to receive a daily injection of rhIGF-I (80 mcg/kg s.c.) or placebo while on conventional insulin therapy for 4 weeks. Insulin was adjusted in the attempt to achieve predetermined goal glycemic values. Free and total IGF-I, four daily blood glucoses, and HbA1c were measured.Before randomization, placebo and rhIGF-I groups exhibited low plasma levels of free and total IGF-I, which increased toward normal levels during the treatment period only in the rhIGF group. The regression curve obtained from the average of daily blood glucose measurements indicated that the glycemic profile, overlapping in the lead-in period, exhibited a downward trend in the rhIGF-I group during the treatment period. Mean blood glucose level during the last 10 days of treatment was lower in the rhIGF-I groups (174 +/- 37 vs. 194 +/- 32 mg/dl). HbA1c level was reduced by more than one-half percent more in the rhIGF-I group (-1.85%) than in the control group (-1.3%). The dose of regular insulin was significantly lower in the rhIGF-I group (0.2 +/- 0.1 vs. 0.28 +/- 0.1 U. kg-1. 10 days-1 in the placebo group; P < 0.05).rhIGF-I in combination with conventional insulin treatment ameliorated the low plasma total and free IGF-I levels and was well tolerated in IDDM. There was a trend toward improved glycemic control, while the regular insulin dose was significantly decreased.
Screening for resistant gram-negative microorganisms to guide empiric therapy of subsequent infection
Authors: Evangelos Papadomichelakis, Flora Kontopidou, Anastasia Antoniadou, Garifalia Poulakou, Evangelos Koratzanis, Petros Kopterides, Irini Mavrou, Apostolos Armaganidis, Helen Giamarellou
Journal: Intensive Care Medicine (2008)
393 ISCHEMIA MODIFIED ALBUMIN AS A EARLY PREDICTOR OF TREATMENT RESPONSE AFTER TRANSARTERIAL CHEMOEMBOLIZATION IN PATIENTS WITH HEPATOCELLULAR CARCINOMA
Authors: C.W. Kim, C.D. Lee, J.W. Jang, S.W. Nam, S.H. Bae, J.Y. Choi, S.H. Cho, S.K. Yoon, J.M. Yang, S.W. Choi, Y.S. Lee
Journal: Journal of Hepatology (2008)