tbuckley/cabot-search huggingface dataset:
Acute effects of amiodarone upon the canine sinus node and atrioventricular junctional region.
Authors: H O Gloor, F Urthaler, T N James
Journal: Journal of Clinical Investigation (1983)

Amiodarone was selectively perfused into the sinus node artery and atrioventricular node artery of 51 dogs. Amiodarone had an immediate negative chronotropic and dromotropic effect. Threshold concentration was 2.5 micrograms/ml. 25 and 50 micrograms/ml of amiodarone injected into the sinus node artery slowed the heart by 25.6 +/- 3.1 and 33.7 +/- 2.6 beats/min (mean +/- 1 SEM), respectively. Amiodarone 25 and 50 micrograms/ml injected into the AV node artery during AV junctional rhythm slowed the AV junctional pacemaker by 12.2 +/- 1.8 and 17.4 +/- 1.7 beats/min, respectively. Injections of amiodarone into the AV node artery during sinus rhythm regularly increased AV conduction time sometimes causing 2 degrees AV block at the highest concentration used. Impaired conduction was exclusively measured at the level of the A-H interval in the His electrogram. Neither atropine nor propranolol prevented the negative chronotropic effects of amiodarone. Amiodarone had no significant effect on sinus node response to either stellate stimulation or intranodal administration of norepinephrine. The negative chronotropic action of amiodarone was significantly enhanced when amiodarone was administered in a perfusate containing low (0.6 mM) instead of normal calcium. Taken collectively these observations indicate that amiodarone has immediate depressant electrophysiologic effects on both the sinus node and the AV junction and that these early effects might involve the blockade of the slow channel.
F-18 FDG Brain Positron Emission Tomography and Tl-201 Early and Delayed SPECT in Distinguishing Atypical Cerebral Tumor from Cerebral Infarction
Authors: HONG-GANG LIU, JAMES M. MOUNTZ
Journal: Clinical Nuclear Medicine (2003)

From the Division of Nuclear Medicine, Department of Radiology, The University of Alabama at Birmingham Medical Center, Birmingham, Alabama Received for publication June 17, 2002. Revision accepted September 12, 2002. Reprint requests: James M. Mountz, Department of Radiology, University of Alabama at Birmingham, 619 19th Street South, JT J260 Birmingham, Alabama 35249-6835. E-mail: [email protected]
BACTERIAL ENDOCARDITIS IN PATIENTS WITH ADULT CONGENITAL HEART DISEASE: THE IMPACT OF LESION TYPE AND RECENT VALVE REPLACEMENT SURGERY
Authors: Darren Mylotte, Dinela Rushani, Liming Guo, Mohammed Kaouache, Kenneth Guo, Judith Therrien, Giuseppe Martucci, Andrew Mackie, Ariane Marelli
Journal: Journal of the American College of Cardiology (2013)
Gynecology in Primary Care: A Step-by-Step Approach
Authors: Nita Walker
Journal: Annals of Internal Medicine (2000)

Medical Writings: Book Notes18 July 2000Gynecology in Primary Care: A Step-by-Step ApproachNita Walker, MDNita Walker, MDUniversity of Cincinnati Medical Center, Cincinnati, Ohio. (Walker)Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-133-2-200007180-00020 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Barbieri RL, Berga SL, DeCherney AH, Hade J, Sheets EE. 105 pages. New York: Scientific American; 1999. $24.95 to subscribers to Scientific American; $34.95 to nonsubscribers. ISBN 0894540327. Order phone 800-545-0554.Field of medicine: General internal medicine.Format: Softcover book.Audience: General internists, family physicians, obstetrician-gynecologists, and trainees.Purpose: To provide a step-by-step approach to diagnosis and cost-effective treatment of problems in ambulatory gynecology.Content: The 20 sections of the book cover the common problems that account for 95% of ambulatory gynecology visits at Brigham and Women's Hospital, Boston, Massachusetts. Each section consists of a detailed, numbered algorithm addressing diagnosis ... Author, Article, and Disclosure InformationAffiliations: University of Cincinnati Medical Center, Cincinnati, Ohio. (Walker) PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 18 July 2000Volume 133, Issue 2Page: 160KeywordsAlgorithmsInfertilityObstetrics and gynecologyPremenstrual syndromeSoftware tools ePublished: 15 August 2000 Issue Published: 18 July 2000 Copyright & PermissionsCopyright © 2000 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Possible Tobramycin Delirium
Authors: Cheryl F. McCartney
Journal: JAMA (1982)

TOBRAMYCIN sulfate and a related aminoglycoside, gentamicin sulfate, are valued for their efficacy in treating serious Gram-negative infections despite their narrow therapeutic ratio.<sup>1</sup>Their widespread use is reflected in 1978 projections from demographic and clinical data that estimated that 3.9 million patients received one course of treatment with an aminoglycoside that year.<sup>2</sup>Reports of serious adverse reactions to these drugs have usually described nephrotoxicity or ototoxicity. Though we can find no published description of tobramycin's corticoneuronal toxicity, there are several case reports of acute toxic psychosis with gentamicin.<sup>3,4</sup>These led us to suspect tobramycin as the cause of delirium in the patient described here. <h3>Report of a Case</h3> The patient was a 66-year-old woman with urinary incontinence. Following radical hysterectomy and radiation therapy for cervical cancer, she had required three surgical procedures for stress urinary incontinence. Intermittent self-catheterization was then offered to relieve the resultant increased residual
Doctors told to shun rewards from industry as size of payments becomes clear
Authors: Fred Charatan
Journal: BMJ (2006)

Doctors in teaching hospitals should lead the way in refusing to take gifts from the pharmaceutical and medical device industries, because it damages the reputation of the profession, a leading article in JAMA said this week (2006;295: 429-33).

The article was published in the month that, during a whistleblower court case originating in Memphis, Tennessee, some spine surgeons were exposed as having accepted hundreds of thousands of dollars from a medical devices company for undertaking limited amounts of work.

The JAMA article, which generated widespread publicity, with articles in the Wall Street Journal , the New York Times , and the Washington Post , pointed …
Inhibition of Rat Brain Na+,K+−ATPase Activity by Serum from Patients with Fulminant Hepatic Failure†
Authors: Hosny W. M. Seda, Robin D. Hughes, Christopher D. Gove, Roger Williams
Journal: Hepatology (1984)

Among the toxins accumulating in the circulation of patients with fulminant hepatic failure (FHF) are substances which inhibit leucocyte ouabain-sensitive sodium transport. A similar inhibition of brain Na+,K+-ATPase could lead to both coma and cerebral edema found in these patients which are associated with high mortality. In this study, we have investigated the effect of sera from FHF on normal rat brain Na+,K+-ATPase activity in vitro. Serum from patients with FHF significantly decreased the ouabain-sensitive Na+,K+-ATPase activity (13.58 +/- S.D. 2.60 mumoles Pi mg protein-1 hr-1) in the rat brain membrane preparation in vitro as compared to normal serum (20.33 +/- 3.24 mumoles Pi mg protein-1 hr-1, p less than 0.001). A final serum dilution of 1 in 40 was required to abolish the inhibition of Na+,K+-ATPase activity. Cerebrospinal fluid obtained at postmortem from FHF patients also contained the inhibitory substances. Serum from patients in coma due to decompensated chronic liver disease inhibited the Na+,K+-ATPase activity (17.25 +/- 1.37 mumoles Pi mg protein-1 hr-1), but this was less marked than with FHF serum. Hence, the inhibition of brain Na+,K+-ATPase by substances accumulating in the serum in FHF may be important in the pathogenesis of hepatic coma.
The effect of prolonged administration of hydroxyurea on morbidity and mortality in adult patients with sickle cell syndromes: results of a 17-year, single-center trial (LaSHS)
Authors: Ersi Voskaridou, Dimitrios Christoulas, Antonios Bilalis, Eleni Plata, Konstantinos Varvagiannis, George Stamatopoulos, Klio Sinopoulou, Aggeliki Balassopoulou, Dimitris Loukopoulos, Evangelos Terpos
Journal: Blood (2009)
Isolated Splenic Metastasis of Ovarian Cancer Detected With 18F-FDG PET/CT
Authors: Dong Hyun Lee, Joon-Kee Yoon, Su Jin Lee, Kyung Sook Jo, Seok-Ho Yoon, Young-Sil An
Journal: Clinical Nuclear Medicine (2013)

We report a case of isolated splenic metastasis from ovarian cancer detected with 18F-FDG PET/CT. A 66-year-old woman with ovarian cancer was referred for 18F-FDG PET/CT to determine the cause of elevated serum CA-125 levels about 48 months after surgery. 18F-FDG PET/CT showed focal, intense uptake in the spleen, with no other abnormal findings. A splenectomy was performed, and the lesion was confirmed as metastasis from ovarian cancer pathologically.
Mutation of TP53 gene is involved in carcinogenesis of hepatic undifferentiated (embryonal) sarcoma of the adult, in contrast with Wnt or telomerase pathways: an immunohistochemical study of three cases with genomic relation in two cases
Authors: Sébastien Lepreux, Sandra Rebouissou, Brigitte Le Bail, Jean Saric, Charles Balabaud, Bertrand Bloch, Marie-Laure Martin-Négrier, Jessica Zucman-Rossi, Paulette Bioulac-Sage
Journal: Journal of Hepatology (2004)
Successful engraftment of HLA-haploidentical related transplants using nonmyeloablative conditioning with fludarabine, busulfan and anti-T-lymphocyte globulin
Authors: H Tamaki, K Ikegame, M Kawakami, T Fujioka, A Tsuboi, Y Oji, Y Oka, H Sugiyama, I Kawase, H Ogawa
Journal: Leukemia (2003)
Temporal pattern of cysteine endopeptidase (cathepsin B) expression in cartilage and synovium from rabbit knees with experimental osteoarthritis: gene expression in chondrocytes in response to interleukin-1 and matrix depletion
Authors: F. Mehraban, M. H Tindal, M. M Proffitt, R. W Moskowitz
Journal: Annals of the Rheumatic Diseases (1997)

<h3>OBJECTIVE</h3> To determine the temporal pattern of expression of cathepsin-B in chondrocytes and synovium in experimental osteoarthritis, and to determine possible mechanisms for upregulation and secretion of cathepsin-B from chondrocytes. <h3>METHODS</h3> Experimental osteoarthritis was induced with partial medial meniscectomy (PM); sham operated (SH) and normal (N) rabbits were used as controls. Cathepsin-B mRNA expression was assessed with northern blotting with a <sup>32</sup>P labelled cDNA probe. Cathepsin-B was measured in conditioned media or cell extracts using a fluorogenic substrate Z-Arg-Arg-AMC. Chondrocyte monolayers were used to determine cathepsin-B expression in response to interleukin-1β (IL-1β). Cartilage explants were used to test the effect of matrix depletion on cathepsin-B release. <h3>RESULTS</h3> Chondrocytes obtained from experimental osteoarthritis knees did not show cathepsin-B mRNA upregulation. However, isolated chondrocytes secreted cathepsin-B into the culture medium. Enzyme release was significantly higher at 8 weeks relative to controls, but not at 12 weeks or 4 weeks. Enzyme released from synovium was significantly higher in PM group compared with SH group at 4 and 8 weeks. IL-1β was ineffective in upregulating steady state cathepsin-B mRNA in chondrocytes; however, it upregulated the intracellular enzyme, and this was blocked with cycloheximide. Enzymatic depletion of cartilage matrix after exposure of explants to IL-1 resulted in release of significantly higher amounts of cathepsin-B into the medium by matrix depleted chondrocytes compared with intact explants. <h3>CONCLUSIONS</h3> In experimental osteoarthritis, cathepsin-B is upregulated in synovial tissue during the early degenerative phase. Progression of experimental osteoarthritis is accompanied by upregulation of cathepsin-B in cartilage. Cartilage and synovial cathepsin-B levels decline as experimental osteoarthritis advances to more degenerative states. IL-1 upregulates intracellular cathepsin-B by increasing cathepsin-B protein synthesis; it is not an effective stimulus for enzyme secretion. Depletion of cartilage matrix during progression of experimental osteoarthritis may contribute to secretion of cathepsin-B and perpetuation of cartilage destruction.
Percutaneous Trephine Lung Biopsy.
Authors: J. R. Wilson
Journal: Annals of Internal Medicine (1972)

Meeting Abstracts1 May 1972Percutaneous Trephine Lung Biopsy.Authors: J. R. Wilson, B.Sc.M., B. Mielke, M.D., and E. G. King, F.R.C.P.Author, Article, & Disclosure InformationPublication: Annals of Internal MedicineVolume 76, Number 5https://doi.org/10.7326/0003-4819-76-5-880_4 Get AccessSubmit a CommentContributors must reveal any conflict of interest. Comments are moderated. Please see our information for authorsregarding comments on an Annals publication.Name (if multiple authors, separate names by comma)Name field is requiredComment TitleTitle field is requiredAffiliation & Institution (max characters: 200)Affiliations field is requiredCommentComment field is requiredPlease disclose any conflicts of interest or leave the box unchecked if you have noneDisclosure of Any Conflicts of InterestCancelSubmit
Electronic patient records: reasons to be cheerful
Authors: Jonathan Gornall
Journal: BMJ (2008)

Like many people, I use the internet for banking, email, shopping, and the transfer of sensitive personal documents, including tax returns. I take sensible precautions—such as declining to give my bank details to phishing fraudsters—and I trust it.

Likewise, I’m relaxed about the prospect of my medical records being available digitally throughout the National Health Service. In fact, as the NHS Summary Care Record pilot scheme approaches its first birthday and enters its evaluation phase, in anticipation of national roll-out, I’m positively excited.

I find it reassuring to think that should I ever find myself in an emergency department, the complete strangers fighting to save my life will have access to any vital personal information that could govern how they treat me.

Also, I find it inspiring to think that the information harvested each year from millions of such encounters will be aggregated and analysed to ensure that the NHS is being managed as safely and as efficiently as possible for the benefit of all.

Helen Wilkinson, a former general practice manager from Hampshire, does not share my equanimity. A few years ago, when she first got a whiff of NHS plans to centralise patient records, she took a look at her own file and discovered that a surgical procedure she had …
Seprase Promotes Rapid Tumor Growth and Increased Microvessel Density in a Mouse Model of Human Breast Cancer
Authors: Yan Huang, Sophie Wang, Thomas Kelly
Journal: Cancer Research (2004)

Abstract Seprase is a cell surface serine protease that is expressed to high levels by invading human breast carcinoma cells. To investigate the role of seprase in breast cancer, MDA MB-231 human mammary adenocarcinoma cells were engineered to express active seprase to high levels. All cells grow rapidly in cell culture. But differences are discovered when the cells are tested for tumorigenicity, growth, and microvessel density by implantation into the mammary fat pads of female severe combined immunodeficient mice. Control transfectants that do not express seprase grow slowly whereas cells that express seprase to high levels form fast-growing tumors that are highly vascular. Microvessel density is elevated in tumors of two different lines of seprase transfectants to 146 ± 67.4 and 144 ± 33.42 vessels/mm2 as compared with 50.5 ± 12.9 vessels/mm2 for tumors of control-transfected cells that do not express seprase. Seprase-expressing cells are better able to attract blood vessels and exhibit rapid tumor growth.
O125 HEPATOCELLULAR CARCINOMA (HCC) INCIDENCE IN CHRONIC HEPATITIS C PATIENTS (CHC) ACCORDING TO SUSTAINED VIROLOGIC RESPONSE (SVR) AND BASELINE CHARACTERISTICS
Authors: T. Purevsambuu, S. Bota, F. Hucke, H. Hofer, P. Ferenci, W. Sieghart, M. Peck-Radosavljevic
Journal: Journal of Hepatology (2014)
Endothelial production of C-type natriuretic peptide and its marked augmentation by transforming growth factor-beta. Possible existence of "vascular natriuretic peptide system".
Authors: S Suga, K Nakao, H Itoh, Y Komatsu, Y Ogawa, N Hama, H Imura
Journal: Journal of Clinical Investigation (1992)

C-type natriuretic peptide (CNP), the third member of the natriuretic peptide family, is thus far known to be distributed mainly in the central nervous system and is considered to act as a neuropeptide, in contrast to atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), which act as cardiac hormones. Recently, we and others have demonstrated that the ANP-B receptor, which is selectively activated by CNP, is localized not only in the central nervous system but in peripheral tissues, including blood vessels. This finding has made us speculate regarding the peripheral production of CNP. In the present study, cultured endothelial cells were examined for CNP production by RIA and Northern blot analysis. CNP-like immunoreactivity was detected in the conditioned media of endothelial cells. Northern blot analysis detected CNPmRNA with a size of 1.2 kb. In addition, transforming growth factor (TGF)-beta, one of the key growth factors for vascular remodeling, markedly stimulated the expression of CNPmRNA and induced a tremendous increase in CNP secretion. We could also detect CNP transcript in the bovine thoracic aorta using the reverse transcription-polymerase chain reaction method. The present study demonstrates the endothelial production of CNP and suggests that a member of the natriuretic peptide family may act as a local regulator in vascular walls. Since evidence for the pathophysiological importance of the vascular renin-angiotensin system has been accumulating and the natriuretic peptide system is known to be antagonistic to the renin-angiotensin system, the possible existence of "vascular natriuretic peptide system" may prove to be of physiological and clinical relevance.
Homogeneity in Pattern of Decline of β-Cell Function in IDDM: Prospective study of 204 consecutive cases followed for 7.4 yr
Authors: Ole Snorgaard, Lassen H Lassen, Christian Binder
Journal: Diabetes Care (1992)

OBJECTIVE To study the natural history of β-cell function from onset of IDDM to expected deterioration of insulin (C-peptide) secretion and to identify different patterns of decline, if any. RESEARCH DESIGN AND METHODS A cohort of 204 consecutive newly diagnosed IDDM (clinical criteria) patients were followed prospectively for 7.4 yr (range 6–9 yr), measuring fasting C-peptide at onset, 1, 3, 6, 9, 12, and then every 6 mo until 106 wk (range 104–135 wk). Then, postprandial C-peptide was measured. RESULTS Fasting C-peptide was 0.17 nM (range 0.11–0.25 nM) at onset followed by an annual increase rate of 0.16 nM/yr (range 0.06–0.48 nM/yr) to a peak of 0.28 nM (range 0.23–0.34 nM/yr) after 25 wk (range 12–39 wk). The subsequent annual decline rate of fasting C-peptide was 0.08 (0.05–0.12) and of postprandial C-peptide 0.03 nM/yr (range 0.02–0.06 nM/yr). None of these parameters showed bimodality in their distribution. However, some parameters were important. In men, fasting C-peptide at onset was lower, but the initial C-peptide increase rate was more pronounced compared to women. Furthermore, insulin-free remission was related to higher C-peptide levels throughout the study. C-peptide was higher during the 1st yr of diabetes in subjects &amp;gt; 30 yr of age at onset compared with younger diabetic patients. Stepwise multiple regression analysis showed that age, male sex, and fasting C-peptide at onset were of some predictive value for the C-peptide levels at 5 yr. However, simple group comparisons revealed no significant differences. CONCLUSIONS No major heterogeneity exists in the pattern of decline of β-cell function in IDDM, although small differences in pattern could be identified in both sexes, in different age-groups, and in relation to achieving insulin-free remission.
Updated Meta-Analysis of Septal Alcohol Ablation Versus Myectomy for Hypertrophic Cardiomyopathy
Authors: Shikhar Agarwal, E. Murat Tuzcu, Milind Y. Desai, Nicholas Smedira, Harry M. Lever, Bruce W. Lytle, Samir R. Kapadia
Journal: Journal of the American College of Cardiology (2010)
Unique Case of Extrauterine Pregnancy
Authors: C. M. Macquibban
Journal: BMJ (1890)

visceral peritoneum covering the tumour, which was then opened and drained by a glass tube.Three deep silk sutures closed the remainder of the incision.From the time of operation the temperature became and re- mained normal, the pain ceased, the appetite returned, and with it the cachexia disappeared and flesh was gained.The stitches were removed on the seventh day, and the tube on the ninth.From the time of operation to the healing of the wound not a drop of pus was seen.On examination per vaginam at the end of a fortnight the uterus was much less fixed, and the cedema of the cervix had disappeared.She was allowed to get up at the end of three weeks, and was discharged cured within a month of the operation.RENEARKS.-Thecases 1 have related were all serious, as these cases usually are, and without active treatment I believe they would all have died, either immediately or remotely, as the result of the pelvic disease; whereas, under the operative treatment here recommended, relief was given in all, and complete and lasting cure was effected for several of the patients.The first, second, and sixth cases are the most remarkable as they are the
Abstract 3913: Establishing a genomic profile of four gastric carcinoma cell lines using array CGH, FISH and cytogenetic analyses
Authors: Xianglan Lu, Gene Hallford, Xianfu Wang, Shibo Li, Ji-Yun Lee
Journal: Cancer Research (2011)

Abstract Four gastric carcinoma cell lines (CRL-1739TM, CRL-5822TM, CRL-5971TM and CRL-5973TM), established in the 1970s and 1980s, were purchased from ATCC. These cell lines have been distributed throughout the world and are regularly used for biomedical research. Cytogenetic information is available for three of these cell lines, and two of the three are known to have complex chromosomal rearrangements, though these have not been well characterized. In part, this is due to the complexity of chromosomal changes and the limited ability of G-banded chromosome analysis. Establishing a genomic profile of these cell lines is vitally important, so that their genotypes and phenotypes can be compared. In the current study, G-banded chromosome analysis, array CGH and FISH analyses were performed on these 4 cell lines. The G-banded chromosome analysis was performed and followed by array CGH using 720k oligonucleotide based array chip. The imbalanced regions and chromosome rearrangements, detected by G-banded chromosome and array CGH analyses, were confirmed by FISH using the appropriate DNA probes. Based on the combined data generated by these assays, we established genomic profiles of each cell line; deletions, duplications, translocations and insertions and a chromosomal homogenous staining region. Deletions of 3p and 18q, duplication of 8q, 19q and extra chromosome 20 were found to be common. These findings are consistent with array CGH analyses of fresh gastric tumor samples (data to be presented separately). The results of this study are important as they will provide important information on the status of genomic profiles, allow the expression patterns of gene(s) to be compared, and provide a mechanism for monitoring the genomic changes in these cell lines. This information, we believe, will allow laboratories to make more informed decisions about the need for replacing existing cell lines due to changes in their biological characteristics. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3913. doi:10.1158/1538-7445.AM2011-3913