tbuckley/cabot-search huggingface dataset:
Systematic use of automated fluorescence‐based sequence analysis of amplified genomic DNA for rapid detection of point mutations
Authors: H. Tamary, S. Surrey, H. Kirschmann, L. Shalmon, R. Zaizov, E. Schwartz, E. F. Rappaport
Journal: American Journal of Hematology (1994)

Abstract Several approaches are now available for screening populations for known mutations in a given gene. However, for detection of multiple mutations in a population that has not been characterized or for detection of new mutations, the value and efficiency of these screening procedures decreases. Although more than 100 different β‐thalassemia mutations have so far been described, the spectrum of mutations in the Eastern Mediterranean and Israel has not been defined in detail. We have used automated fluorescence‐based DNA sequence analysis of PCR‐amplified genomic DNA employing a cycle‐sequencing strategy coupled with advanced analysis software to rapidly detect β‐thalassemia mutations in Israeli patients. This method enabled rapid identification of eight different mutations in 10 patients, including two rare mutations, one of which has never been described in this geographic region. Our results show that automated fluorescence‐based DNA sequence analysis of amplified genomic DNA is a rapid and reliable method for detection of point mutations and small deletions or insertions in both heterozygous and homozygous states. This approach is particularly effective for a relatively small gene such as β‐globin, but it can also be used for rapid detection of mutations in large genes by first sequencing clusters of exons and intron/exon borders. © 1994 Wiley‐Liss, Inc.
Deep brain stimulation restores frontostriatal network activity in obsessive-compulsive disorder
Authors: Martijn Figee, Judy Luigjes, Ruud Smolders, Carlos-Eduardo Valencia-Alfonso, Guido van Wingen, Bart de Kwaasteniet, Mariska Mantione, Pieter Ooms, Pelle de Koning, Nienke Vulink, Nina Levar, Lukas Droge, Pepijn van den Munckhof, P Richard Schuurman, Aart Nederveen, Wim van den Brink, Ali Mazaheri, Matthijs Vink, Damiaan Denys
Journal: Nature Neuroscience (2013)
Demonstration of Collateral Pathways in Superior Vena Cava Syndrome by Means of Radionuclide Venography
Authors: TOSHIYUKI KIDA
Journal: Clinical Nuclear Medicine (1985)

Radionuclide venography was used to study lesions producing obstruction in the superior vena caval system. This procedure is a simple and safe diagnostic method; it offers precise information regarding the site and degree of venous obstruction and the extent of the collaterals. It is useful not only in diagnosis, but also in radiotherapy planning and follow-up evaluation.
BBC accused of unethical journalism
Authors: O. Dyer
Journal: BMJ (2003)

Britain's most prominent assisted reproduction specialist, Professor Ian Craft, has accused the BBC investigative news programme Panorama of using unethical journalism in an attempt to smear him as an irresponsible doctor.

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Professor Ian Craft: BBC has breached its own guidelines

Credit: TOBY MELVILLE/PA

Professor Craft says that Panorama 's producers sent an undercover journalist posing as a patient to his Harley Street clinic, the London Fertility Centre, in contravention of BBC guidelines. The visit was …

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Fortnightly review: Intracoronary stents
Authors: M. M Gandhi, K. D Dawkins
Journal: BMJ (1999)

Thank you for your interest in spreading the word about The BMJ.NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address. Username * Your Email * Send To * You are going to email the following Intracoronary stents Your Personal Message CAPTCHA This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Acute transverse myelopathy complicating systemic lupus erythematosus.
Authors: D J Propper, R C Bucknall
Journal: Annals of the Rheumatic Diseases (1989)

A sixteen year old girl with systemic lupus erythematosus developed acute transverse myelopathy. She was treated with high dose steroids, cyclophosphamide, and plasma exchange and regained partial neurological function. Previous descriptions of transverse myelopathy complicating systemic lupus erythematosus are reviewed, with particular reference to the efficacy of high dose steroid treatment.
Gene Transfer to Repopulating Human CD34+ Cells Using Amphotropic-, GALV-, or RD114-Pseudotyped HIV-1-Based Vectors from Stable Producer Cells
Authors: Thomas Relander, Maria Johansson, Karin Olsson, Yasuhiro Ikeda, Yasuhiro Takeuchi, Mary Collins, Johan Richter
Journal: Molecular Therapy (2004)

A novel, stable human immunodeficiency virus type 1 vector packaging system, STAR, was tested for its ability to transduce human cord blood CD34+ progenitor cells assayed both in vitro and after transplantation into NOD/SCID mice. Vectors pseudotyped with three different gammaretrovirus envelopes were used: the amphotropic MLV envelope (MLV-A), a modified gibbon ape leukemia virus envelope (GALV+), and a modified feline endogenous virus RD114 envelope (RDpro). Gene transfer to freshly thawed CD34+ cells in the absence of cytokines was very low. Addition of cytokines increased gene transfer efficiency significantly and this was further augmented if the cells were prestimulated for 24 h. Concentration of the vectors (15-fold) by low-speed centrifugation increased gene transfer to CD34+ cells in vitro even further. More than 90% of cells were transduced with a single exposure to the RDpro vector as determined by GFP expression using flow cytometry. The two other pseudotypes transduced approximately 65-70% of the cells under the same conditions. Transplantation of CD34+ cells prestimulated for 24 h and then transduced with a single exposure to concentrated vector revealed that the RDpro vector transduced 55.1% of NOD/SCID repopulating human cells, which was significantly higher than the MLV-A (12.6%)- or GALV+ (25.1%)-pseudotyped vectors.
Two New Causes of Alopecia
Authors: Stuart M. Copperman
Journal: JAMA (1984)

To the Editor.—  In any generation, new life-styles produce new afflictions. Two young men recently came to my office with patchy baldness in the occiptoparietal area. These boys, aged 17 years, had been practicing breakdancing. The constant spinning on the top of the head had eroded the hair shafts down to the scalp.  A young woman was noted by her family to have linear transverse hair loss in the same general area. She had been jogging daily, using a rather tight-banded, wide-stripped heavy headphone. Switching to a lighter headpiece stopped the hair loss.  May we contribute to the medical lexicon two new diseases—alopecia breakdancia and alopecia walkmania?
Uncertain benefit: investigators' views and communications in early phase gene transfer trials
Authors: Gail E. Henderson, Arlene M. Davis, Nancy M.P. King, Michele M. Easter, Catherine R. Zimmer, Barbra Bluestone Rothschild, Benjamin S. Wilfond, Daniel K. Nelson, Larry R. Churchill
Journal: Molecular Therapy (2004)

We report on a study of potential sources of therapeutic misconception in early phase gene transfer research, examining how investigators and their consent forms represent the prospect for direct benefit. Our analysis demonstrates that even though half of PIs said they expected direct medical benefit for their subjects, they did not necessarily convey this to their subjects. What they reported telling subjects resembled what was written in their consent form, which suggests that, far from being irrelevant, the consent form is an influential component of the consent process. We also demonstrate that the language used to describe direct benefit in consent forms and PIs' discussions was mostly vague, ambiguous, and indeterminate about benefit, rather than clearly negative. This was especially true for cancer and vascular disease trials. Our respondents found the problem of balancing hopes and expectations, for themselves and for their subjects, extraordinarily challenging. In the current era, investigators face such challenges without consistent normative guidance or agreed-upon standards for how to talk about scientific promise and uncertainty in early phase trials. This dilemma cannot be effectively addressed by individual investigators alone, but must be acknowledged and openly discussed by the scientific community at large.
OPTIC AND PERIPHERAL NEURITIS
Authors: Robert J. T. Joy
Journal: JAMA (1960)

Chloramphenicol is one of the most valuable antibiotics available to the physician,<sup>1</sup>although in rare instances it may cause severe hematopoietic reactions.<sup>2</sup>It is the purpose of this paper to emphasize another apparent severe toxic reaction—the occurrence of optic and peripheral neuritis. <h3>Report of a Case</h3> The patient was a 20-year-old man who had been in good health until Oct. 21, 1957, when he was treated with penicillin for a "flu-like" syndrome. Three days later genitourinary symptoms occurred; he became systemically ill and was hospitalized. Physical examination at that time showed only costovertebral angle tenderness. He had marked pyuria, and his chest roentgenogram showed a small infiltrate in the upper lobe of his left lung. He was treated with chlortetracycline and his genitourinary symptoms subsided. On Nov. 7, pain developed in his left shoulder, but roentgenograms were normal. A nonproductive arthrocentesis was performed, with the instillation of penicillin.
The Location of New Medical Schools
Authors: Robert D. Tschirgi
Journal: JAMA (1971)

Given the social ambience and economic structure of the United States now and for the coming decades, it appears most likely that the location of new medical schools will be decided through a combination of local initiative and national design, but with increasing emphasis on national design. Before this general statement can be meaningfully discussed, however, it is necessary to consider the definitions of the three key elements: medical schools, local initiative, and national design. <h3>Medical Schools</h3> The answer to "What is a medical school?" like the answer to "What is beauty?" seems to lie in the eye of the beholder, and different beholders have widely varying and vigorously espoused opinions. At one end of the spectrum, a medical school is defined as a constellation of teachers and undergraduate MD candidates, supported by classrooms, teaching laboratories, and hospital beds. The function of such a school is largely and, in the
STUDIES ON TUBERCULIN FEVER
Authors: William J. Hall, Lorraine Francis, Elisha Atkins
Journal: The Journal of Experimental Medicine (1970)

Utilizing techniques of passive transfer, we have investigated the factors responsible for production of fever when tuberculin is given intravenously to specifically sensitized rabbits. The ability to develop a febrile response to tuberculin could be passively transferred to normal recipients with viable mononuclear cells from peritoneal exudates, spleen, or lymph nodes of donor rabbits sensitized with BCG. Sensitivity was usually apparent 48 hr after transfer, maximal at 7 to 14 days, and rapidly declined thereafter. Granulocytes and nonviable, sonicated, mononuclear cells from similarly sensitized donors were unable to transfer this form of reactivity. Passive transfer of reactivity was also effected with plasma and serum, suggesting that the reaction of antibody with antigen contained in tuberculin is one of the initial steps by which the host cells are activated to release the endogenous pyrogen (EP) that mediates this form of hypersensitivity fever. An intravenous infusion of granulocytes, as well as of several types of mononuclear cells from sensitized donors, made most recipients responsive to the pyrogenic effect of old tuberculin (OT) given 2 hr later. Some of these passively transferred cells, such as the granulocyte and alveolar macrophage, may be activated in vivo by OT, as they are in vitro. However, in the case of splenic and lymph node cells that cannot be activated by OT to produce EP in vitro, it seems likely that an intravenous injection of OT causes these transferred, sensitized cells to liberate an intermediate substance that either directly, or in association with antigen, activates the host's normal cells to produce EP. In support of previous suggestions that leukocytes of several types, as well as phagocytic cells of the reticuloendothelial system, serve as potential sources of EP in tuberculin-induced fever, evidence was presented that OT also activates both granulocytes and mononuclear cells from sterile exudates of BCG-sensitized donors to produce EP in vitro.
The Certitudes and Uncertainties of Health Care Reform
Authors: Robert B. Doherty
Journal: Annals of Internal Medicine (2010)

The Patient Protection and Affordable Care Act (PPACA) of 2010 was signed into law by President Obama on March 23. This legislation has elicited much debate among policy experts and the public alike. No one knows exactly how this new complex law will play out, and objective evaluation of its effects is important. The American College of Physicians hopes that the legislation will advance key priorities on coverage, workforce, and payment and delivery system reform. The goal of the PPACA is to help provide affordable health insurance coverage to most Americans, improve access to primary care, and lower costs. This article discusses what the chances are that it will accomplish these objectives. It also explains many of the key provisions in the legislation and how they will affect both physicians and patients. Despite considerable uncertainty about the effects of this act, when compared with the status quo, it is an extraordinary achievement that will continue to evolve through its implementation.
Absorption of phenol red from the human lung.
Authors: J L Maddocks
Journal: Thorax (1975)

Pulmonary absorption of phenol red was studied in normal subjects. Phenol red was administered by intratracheal injection and its urinary excretion was used as an index of pulmonary absorption. Doses ranging from 3 to 30 mg were given to two subjects and urinary phenol red excretion was found to be rate limited. That this effect occurred in the lung was shown by giving the dye intravenously to one subject. A linear relationship between dose and urinary excretion was then observed. Intratracheal p-aminohippurate did not reduce pulmonary absorption of phenol red in one subject. Pulmonary absorption of phenol red dissolved in 0-9% saline and 0-18% saline was compared in nine subjects. Dye absorption was three times greater when it was given in 0-18% saline. When the saline concentration of phenol red doses was held constant there was a linear relationship between the intratracheal dose and urinary excretion in one subject. These results suggest that phenol red is absorbed from the lung by passive diffusion. They also show the importance of solvent effects when studying pulmonary absorption of a substance.
Abstract 381: Non-invasive histology for early detection of cutaneous melanoma and pigmented lesions <i>in vivo</i>
Authors: Simone Degan, Jesse W. Wilson, Tanya E. Mitropoulos, Jennifer Y. Zhang, Maria A. Selim, Warren S. Warren
Journal: Cancer Research (2012)

Abstract Rationale The diagnosis of melanoma is based on clinical identification followed by histopathologic confirmation. Surgical excision remains the mainstay for a suspicious lesion that will be evaluated for the morphological and cytological signatures of melanomas. However, in some patients such as those with atypical mole syndrome, multiple biopsies of each lesion can be burdensome. We have previously reported that imaging the distribution of eumelanin and pheomelanin can be used to detect melanomas from benign lesions (Matthews TE et al. Sci Transl Med Feb 23;3(71)). In this pre-clinical study we extend our investigation in vivo using nonlinear pump-probe microscopy. We assess cellular morphology and detect the expression of eumelanin and pheomelanin, the two forms of melanin found in skin as markers of melanoma progression. Methods Two melanoma cells lines (A2058; SK-MEL-28) were incubated in human skin dermis. Grafts were xenotransplanted onto SCID mice (n=13, A2058; n=6, SK-MEL-28; n=2 control) and imaged every 8-10-days for cancer progression and morphological changes. Pump-probe microscopy was performed concurrently with two other established laser scanning microscopy methods, multiphoton autofluorescence (MPAF) and reflectance confocal scanning laser microscopy (RCSLM). After 4-8 weeks the subjects were euthanized and the tumor excised for histological examination. Hematoxylin and eosin staining was performed to confirm the presence of the tumor and to correlate with the morphological findings after laser image acquisition. Results The in vivo pump-probe images of the cancer subjects showed an irregular distribution of the pigment, lacking the organized structure typical of skin. Pump-probe images of both melanoma cell lines indicated pigmentation that was either primarily eumelanin or a mixture of eumelanin and pheomelanin with marked spatial heterogeneity. Pigmentation was observed in the two control mice but localized on the basal cells with a well-organized cellular architecture. At the end of the cancer subjects’ life the histological examination showed atypical melanocytes mainly present as single cells in the dermal area. Hyperplasia was also present in the dermal compartment and in the dermal-epidermal junction of the skin. Conclusions The present work may open new avenues for the non-invasive detection of cellular and pigment lesions to prevent melanoma development and invasiveness. Such method can play a role in the diagnosis of melanoma in those patients with multiple lesions or in the areas of the body where the lesion is very exposed such as the face or neck and in ocular region. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 381. doi:1538-7445.AM2012-381
[390] WHAT IS THE HEMATOLOGICAL POTENTIAL OF ADULT NON HUMAN PRIMATE HEPATIC CELLS?
Authors: J.F. Mayol, M. Drouet, C. Delaunay, F. Mourcin, P. Garrigou, N. Grenier, C. Letoublon, F. Norol, I.P. Zarski, F. Herodin
Journal: Journal of Hepatology (2007)
Improving Survival After Renal Transplantation for Diabetic Patients with Severe Coronary Artery Disease
Authors: John A D'Elia, Larry A Weinrauch, Antoine Kaldany, John A Libertino, O Stevens Leland, Robert W Healy, Donald G Miller
Journal: Diabetes Care (1981)

After successful renal transplantation, seven diabetic renal failure patients with severe coronary artery disease returned to productive employment. Despite the requirement for additional peripheral vascular or ophthalmologic surgery in four patients, their renal function remained adequate. Following transplantation, diabetic complications included angina in three, myocardial infarction in three, and cerebrovascular accident in two patients. Two patients with adequate renal function died suddenly at 29 and 62 mo. Despite severe coronary artery disease, an increasing number of diabetic dialysis patients may be able to return to work after a successful kidney transplant.
INTRA-EPIDERMAL CARCINOMA OF THE SKIN.*
Authors: LOUIS SAVATARD
Journal: British Journal of Dermatology (1931)

Journal Article INTRA‐EPIDERMAL CARCINOMA OF THE SKIN. Get access LOUIS SAVATARD LOUIS SAVATARD Manchester Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology and Syphilis, Volume 43, Issue 4, 1 April 1931, Pages 161–168, https://doi.org/10.1111/j.1365-2133.1931.tb09484.x Published: 01 April 1931
Benefit of initial resection of hepatocellular carcinoma followed by transplantation in case of recurrence: An intention-to-treat analysis
Authors: David Fuks, Safi Dokmak, Valérie Paradis, Momar Diouf, François Durand, Jacques Belghiti
Journal: Hepatology (2011)

Liver resection (LR) for hepatocellular carcinoma (HCC) as the first–line treatment in transplantable patients followed by “salvage transplantation” (ST) in case of recurrence is an attractive concept. The aim was to identify patients who gain benefit from this approach in an intention–to–treat study. From 1998 to 2008, among 329 potential candidates for liver transplantation (LT) with HCC within the Milan criteria (MC), 138 with good liver function were resected (LR group) from a perspective of ST in case of recurrence, and 191 were listed for LT first (LT group). The two groups were compared on an intention–to–treat basis with special reference to management of recurrences and transplantability after LR. Univariate and multivariate analyses were performed to identify resected patients who developed recurrence beyond MC. Five–year overall and disease–free survival was similar in both groups: LT versus LR group, 60% versus 77% and 56% versus 40%, respectively. Among the 138 patients in the LR group, 20 underwent LT before recurrence, 39 (28%) had ST, and 51 (37%) with recurrence were not transplanted including 21 within MC who were excluded for advanced age, acquired comorbidities, or refusal and 30 (22%) with recurrence beyond MC. Predictive factors for nontransplantability due to recurrence beyond MC included microscopic vascular invasion (hazard ratio [HR] 2.38 [range, 1.10–7.29]), satellite nodules (HR 2.46 [range, 1.01–6.68]), tumor size &gt; 3 cm (HR 1.34 [range, 1.03–3.12]), poorly differentiated tumor (HR 3.18 [range, 1.31–7.70]), and liver cirrhosis (HR 1.90 [range, 1.04–3.12]). Conclusion : The high risk of failure of ST after initial LR for HCC within MC suggests the use of tissue analysis as a selection criterion. The salvage LT strategy should be restricted to patients with favorable oncological factors. (Hepatology 2012;;55:132–140)
Coexpression of CD9 augments the ability of membrane-bound heparin-binding epidermal growth factor-like growth factor (proHB-EGF) to preserve renal epithelial cell viability
Authors: Tsukasa Takemura, Satoshi Hino, Yuka Murata, Hidehiko Yanagida, Mitsuru Okada, Kazuo Yoshioka, Raymond C. Harris
Journal: Kidney International (1999)
Risk Factors for Amputation in Diabetics
Authors: Gayle E. Reiber
Journal: Annals of Internal Medicine (1993)

Letters1 February 1993Risk Factors for Amputation in DiabeticsGayle E. Reiber, MD and Thomas D. Koepsell, MDGayle E. Reiber, MDSearch for more papers by this author and Thomas D. Koepsell, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-118-3-199302010-00020 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail IN RESPONSE:Our inclusion criterion was an “initial amputation of the affected lower extremity” [1]. We did not study the preamputation treatment strategy and factors leading to the decision to amputate. We did, however, address many health care provider and patient risk factors where intervention could minimize the risk for limb loss.As for infection in the cases and controls, including osteomyelitis, 68% of cases had an active infection of the affected lower extremity [1]. Infection was dichotomized as present or absent, based on physical examination, microbiologic culture results, radiologic studies, and pathology reports. The criteria for control selection excluded ...REFERENCE1. Reiber GE, Pecoraro RE, Koepsell TD. Risk factors for amputation in patients with diabetes mellitus: a case–control study. Ann Intern Med. 1992; 117:97-105. Google Scholar Author, Article, and Disclosure InformationAuthors: Gayle E. Reiber, MD; Thomas D. Koepsell, MDAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 February 1993Volume 118, Issue 3Page: 231KeywordsHealth care providersMedical risk factorsOsteomyelitisUlcers ePublished: 15 August 2000 Issue Published: 1 February 1993 Copyright & PermissionsCopyright © 1993 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...