tbuckley/cabot-search huggingface dataset:
Comparison of direct immunostaining and electroimmunoassay for analysis of airborne grass‐pollen antigens
Authors: E. Yli‐Panula, Y. Takahashi, A. Rantio‐Lehtimäki
Journal: Allergy (1997)

Yli‐Panula E, Takahashi Y, Rantio‐Lehtimäki A. Comparison of direct immunostaining and electroimmunoassay for analysis of airborne grass‐pollen antigens. Sensitive pollen‐allergic patients have been reported to show allergic symptoms not only during the pollen release of allergenic plants but also both before and after the pollen season. Symptoms before the season are evidently provoked by small‐sized particles originating partly from developing pollen grains, partly from other plant parts. After the pollen season, antigenic material settles on various surfaces, which thus form a new source of allergenic material. Measuring the allergen concentrations in indoor and outdoor environments demands an effective sampling method and a rapid and sensitive immunochemical analysis, especially for particles of small‐sized fractions which are not detected in microscopic analyses. The efficiency of an ELISA and an immunochemical staining method was tested with monoclonal IgG against Phl p 5, the main grass allergen. The Burkard trap and MPC impactor ‘Marple personal cascade impactor with six‐stage particle size fractionation’ were compared. The sampling was carried out in southwestern Finland in the summer of 1994. The number of grass‐pollen antigen spots greatly exceeded the simultaneous pollen count, indicating considerable antigen activity outside the pollen grains. The counts were especially high in small‐sized fractions after the pollen season, when hardly any airborne pollen was found. Spots and pollen divided according to size were highly intercorrelated, indicating that the threshold values used were appropriate.
HHS Public Records
Authors: Mike Mitka
Journal: JAMA (2013)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
ASSOCIATION OF PELVIC DISEASES AND INSANITY IN WOMEN, AND THE INFLUENCE OF TREATMENT OF THE LOCAL DISEASE UPON THE MENTAL CONDITION.
Authors: H. A. TOMLINSON
Journal: JAMA (1899)

In January, 1894, there was begun in the St. Peter State Hospital a systematic inquiry into the relation between insanity in our women patients and whatever disturbance of function or disease of the generative organs there might be present. In order to accomplish this purpose, a careful record was made of the incidents of the menstrual period in each case; with reference to the amount and character of the discharge, the length of the period, the presence or absence of pain, headache, or general disturbance; and finally as to whether there was any change or exaggeration of the mental disturbance during the period. Then each patient was subjected to a careful physical examination and a record made of the conditions found. In all of the cases where pelvic disease was present and apparent, and in those cases in which there was menstrual disorder, a systematic study of the conditions found,
Kidney Transplantation: A Comparison of Results Using Cadaver and Related Living Donors.
Authors: R. Goldman
Journal: Annals of Internal Medicine (1968)

s1 May 1968Kidney Transplantation: A Comparison of Results Using Cadaver and Related Living Donors.R. Goldman, M.D., F.A.C.P., C. E. Reeve, M.D., D. C. Martin, M.D., H. C. Gonick, M.D., F.A.C.P., J. J. Kaufman, M.D., M. E. Rubini, M.D., F.A.C.P., M. M. Mims, M.D., W. E. Goodwin, M.D.R. Goldman, M.D., F.A.C.P.Search for more papers by this author, C. E. Reeve, M.D.Search for more papers by this author, D. C. Martin, M.D.Search for more papers by this author, H. C. Gonick, M.D., F.A.C.P.Search for more papers by this author, J. J. Kaufman, M.D.Search for more papers by this author, M. E. Rubini, M.D., F.A.C.P.Search for more papers by this author, M. M. Mims, M.D.Search for more papers by this author, W. E. Goodwin, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-68-5-1180_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptNinety-four renal homotransplants have been performed at the UCLA and Los Angeles Veterans Administration Hospitals since January 1964; 47 from living donors (LD) and 47 from cadaver donors (CD). Patients were selected according to the usual criteria and were divided into the two groups depending on the availability of a suitable donor. Because of this the average age of the CD group (36.2 years) was greater than that of the LD group (27.5 years). Most of the LD group underwent bilateral nephrectomy at the time of transplant, while the CD group did not. Otherwise, operative technique, posttransplant treatment, and follow-up... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Los Angeles, California PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 May 1968Volume 68, Issue 5Page: 1180-1181KeywordsNephrectomyRenal transplantation ePublished: 1 December 2008 Issue Published: 1 May 1968 PDF downloadLoading ...
Laboratory control of oral anticoagulants.
Authors: L Poller
Journal: BMJ (1987)

year at school, as well as to their parents.A few genetic clinics are necessary to provide this service; ophthalmological support is essential for an accurate diagnosis, itself a prerequisite for meaningful genetic counselling.The "new genetics," with prenatal diagnosis by chorion biopsy of genetically determined disorders, has dramatically changed the prospects of secondary prevention.Among the conditions that may now be diagnosed early in pregnancy are X linked retinitis pigmentosa,9 choroideraemia,'0 and retinoblastoma."IThis list should increase over the next few years, with additional-autosomal recessive, autosomal dominant, and X linked conditions.Hence secondary prevention of genetically determined diseases will become increasingly important.This is yet another reason why genetic counselling should be made available to all visually handicapped children and their families.
How Large Employers Are Shaping the Health Care Marketplace
Authors: T Bodenheimer, K Sullivan
Journal: New England Journal of Medicine (1998)

During the past 15 years, the U.S. health care system has undergone breathtaking changes. It is no exaggeration to call this process a revolution. Authority was wrested from physicians and hospitals and conferred on health maintenance organizations (HMOs) and other managed-care insurers. The revolutionaries are not the downtrodden — low-income and disabled patients or underpaid hospital and nursing home workers. The revolutionaries are America's multibillion-dollar Fortune 500 companies. They set off the insurrection in the 1980s by inducing or requiring their employees to obtain health insurance from managed-care insurers, especially HMOs. Whether employers will allow power to remain in the . . .
Identification of Mechanisms Underlying Transporter Associated with Antigen Processing Deficiency in Metastatic Murine Carcinomas
Authors: A. Francesca Setiadi, Muriel D. David, Susan S. Chen, John Hiscott, Wilfred A. Jefferies
Journal: Cancer Research (2005)

Abstract Expression of transporter associated with antigen processing (TAP) is often lost in metastatic carcinomas, resulting in defective antigen processing and presentation and escape of the cancer cells from immune surveillance. In this study, the nature of TAP deficiencies in tumors was investigated. By chromatin immunoprecipitation assay, we showed that the recruitment of RNA polymerase II to the TAP-1 gene was impaired in TAP-deficient cells derived from murine melanoma, prostate, and lung carcinomas, compared with TAP-expressing fibroblasts and lymphoma cells. This suggested that the deficiency in TAP-1 expression resulted, at least partially, from a relatively low level of transcription of the TAP-1 gene. Furthermore, levels of TAP-1 promoter activity, as assessed by stable transfections with a reporter construct containing the TAP-1 promoter, were relatively low in TAP-deficient cells. To examine genetic heritability of regulators of TAP-1 promoter activity, TAP- and MHC class I–deficient cells of H-2b origin were fused with wild-type fibroblasts of H-2k origin. Fusion with TAP-expressing cells complemented the low levels of TAP-1 promoter activity in TAP-deficient cells. However, these fused cells exhibited lower levels of TAP-1 mRNA and H-2k than unfused fibroblasts. Further analysis showed that TAP-1 mRNA stability was lower in fused carcinoma fibroblasts than in unfused fibroblasts. Based on these results, we propose that TAP deficiency in many carcinomas is caused by a decrease in activity/expression of trans-acting factors regulating TAP-1 promoter activity, as well as a decrease in TAP-1 mRNA stability. These results have significant implications for understanding immune evasion mechanisms in tumors.
Sodium Output—Blood Pressure Relationships and Their Modification by Treatment
Authors: D. M. GREEN, EUGENE J. ELLIS
Journal: Circulation (1954)

This study summarizes relationships among blood pressure, 24-hour sodium output, basal output and output under load, in patients on self-selected diets. Additional evidence was secured for the existence of a general correlation between blood pressure and sodium output. This correlation persisted when the blood pressure of hypertensive patients was chronically reduced by medical or surgical therapy.
The effects of JNJ-26481585, a novel hydroxamate-based histone deacetylase inhibitor, on the development of multiple myeloma in the 5T2MM and 5T33MM murine models
Authors: S Deleu, M Lemaire, J Arts, E Menu, E Van Valckenborgh, P King, I Vande Broek, H De Raeve, B Van Camp, P Croucher, K Vanderkerken
Journal: Leukemia (2009)
Viral infection may lead to vessel wall problems
Authors: Phil Gunby
Journal: JAMA (1984)

A link was drawn between viruses, vasculitis, and possibly thrombosis in other research on the inflammatory process reported at the American Heart Association's Florida forum Douglas B. Cines, MD, University of Pennsylvania School of Medicine, Philadelphia, says he and colleagues used human umbilical vein endothelial cell cultures to "establish that infection with herpes simplex type 1 virus programs the endothelial cell to produce large quantities of viral proteins." As the virus replicates within the endothelium, he says, "these viral proteins become inserted into the surface of the endothelial cell, where they bind antibodies and/or complement-coated immune complexes. This enhanced binding of immune complexes could attract activated complement proteins and leukocytes from the circulation. In turn, these two systems are capable of initiating inflammation." Furthermore, Cines says, the resulting "immunologic endothelial cell injury could initiate platelet adherence. In turn, this adherence may lead to the thromboses that occur in certain vasculitic
p73 Isoforms Can Induce T-Cell Factor–Dependent Transcription in Gastrointestinal Cells
Authors: Katarina Tomkova, Abbes Belkhiri, Wael El-Rifai, Alexander I. Zaika
Journal: Cancer Research (2004)

Abstract A new p53 family member, p73, and its isoform ΔNp73 are increasingly recognized in cancer research as important players in tumorigenesis, as well as in chemotherapeutic drug sensitivity. Despite substantial structural similarities to p53, accumulating evidence suggests that p53 and p73 may play different roles in human tumorigenesis. In this study, we have investigated the role of p73 and ΔNp73 in upper gastrointestinal tumorigenesis. Our results indicate that p73 and ΔNp73 are frequently overexpressed in &amp;gt;60% of primary adenocarcinomas of the stomach and esophagus. We have demonstrated that this overexpression can lead to the suppression of p73 transcriptional and apoptotic activity in gastrointestinal cells. Moreover, it induces β-catenin up-regulation and T-cell factor/lymphocyte enhancement factor–dependent transcription. Wild-type p53, but not mutant p53, can inhibit this effect. Our results demonstrate a novel mechanism for activation of β-catenin in gastrointestinal tumors and support the concept that overexpression of p73 isoforms can play an important role in tumorigenesis.
The Effect of Glucose Variability on the Risk of Microvascular Complications in Type 1 Diabetes
Authors: Irl B. Hirsch, Michael Brownlee
Journal: Diabetes Care (2006)

P ϭ 0.05) was observed when SDs of seven-point glycemic profiles were substituted for MAGE values.Even though the MAGE determination requires continuous glucose monitoring, we believe that this parameter should be the "gold standard" for assessing glucose fluctuations in all prospective interventional studies designed to estimate glucose variability.We therefore believe that additional studies are required to definitively determine the role of glycemic variability in the pathogenesis of the micro-and macrovascular complications of diabetes.Even though the technology of continuous measurements of glucose in interstitial fluid remains
Risk Factors for Cutaneous Melanoma
Authors: Arthur R. Rhodes
Journal: JAMA (1987)

Cutaneous melanoma is rapidly becoming a potentially curable cancer if it is detected and properly treated in an early phase of development. Unlike other cancers, which are usually hidden from detection until they are relatively large or metastatic disease has occurred, cutaneous melanoma is readily detectable simply by examining the skin. Information is now available that will be useful in selecting individuals at greatest risk. The most important melanoma risk factors (in decreasing order of importance) for a given individual are as follows: a persistently changed or changing mole, adulthood, irregular varieties of pigmented lesions (including dysplastic moles and lentigo maligna), a congenital mole, Caucasian race, a previous cutaneous melanoma, a family history of cutaneous melanoma, immunosuppression, sun sensitivity, and excessive sun exposure. Selective screening and appropriate treatment of individuals who have these risk factors may reduce the morbidity and mortality of cutaneous melanoma.
Relations of Diseases of the Eye to General Diseases.
Authors: Max Knies Knies, Max Knies
Journal: JAMA (1895)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Novel Mutants Define Genes Required for the Expression of Human Histocompatibility Leukocyte Antigen DM: Evidence for Loci on Human Chromosome 6p
Authors: Steven P. Fling, Jennifer Rak, Kimberly A. Muczynski, Benjamin Arp, Donald Pious
Journal: The Journal of Experimental Medicine (1997)

We and others have shown that the products of the HLA-DM locus are required for the intracellular assembly of major histocompatibility complex class II molecules with cognate peptides for antigen presentation. HLA-DM heterodimers mediate the dissociation of invariant chain (Ii)-derived class II–associated Ii peptides (CLIP) from class II molecules and facilitate the loading of class II molecules with antigenic peptides. Here we describe novel APC mutants with defects in the formation of class II–peptide complexes. These mutants express class II molecules which are conformationally altered, and an aberrantly high percentage of these class II molecules are associated with Ii-derived CLIP. This phenotype resembles that of DM null mutants. However, we show that the defects in two of these new mutants do not map to the DM locus. Nevertheless, our evidence suggests that the antigen processing defective phenotype in these mutants results from deficient DM expression. These mutants thus appear to define genes in which mutations have differential effects on the expression of conventional class II molecules and DM molecules. Our data are most consistent with these factors mapping to human chromosome 6p. Previous data have suggested that the expression of DM and class II genes are coordinately regulated. The results reported here suggest that DM and class II can also be differentially regulated, and that this differential regulation has significant effects on class II–restricted antigen processing.
Are patients on treatment for pulmonary TB who stop expectorating sputum genuinely culture negative?
Authors: F M R Perrin, R A M Breen, T D McHugh, S H Gillespie, M C I Lipman
Journal: Thorax (2009)

We previously reported the basic performance of a prototype small cadmium telluride (CdTe) γ-camera (SSGC) intended for use in radioguided surgeries. In this study, we sought to confirm the favorable previous results and to extend the preliminary findings to examine the efficacy of the SSGC in an animal study and a clinical setting for sentinel lymph node biopsy. <b>Methods:</b> The prototype SSGC (1,024 pixels; field of view, 44.8 × 44.8 mm), equipped with a parallel-hole collimator, was used in both animal and clinical studies. <sup>99m</sup>Tc-phytate (18.5 MBq) was injected into the tongues and legs of 6 rabbits. In the clinical study, 74 MBq of <sup>99m</sup>Tc-phytate was injected into peritumoral regions in 8 patients with oral cancer. The detection of hot nodes by the SSGC was compared with that by a conventional scintillation γ-camera (CGC). <b>Results:</b> The SSGC detected 29 hot nodes in images of 6 rabbits after injection. The number of hot nodes was the same as the number seen in CGC studies, but the CGC required a longer acquisition time to produce comparable images. There were no differences between the SSGC and the CGC in terms of activity ratios and hot node-to-background ratios. The biodistribution of <sup>99m</sup>Tc-phytate in removed tissues was evaluated by contact radiography, and radioactivity was assayed with a γ-well counter. The mean ± SD radioactivity in specimens was 0.15% ± 0.15%, with a range of 0.01%–0.62%. In the clinical study, the SSGC detected 30 hot nodes with a 5- to 60-s acquisition time at 4 h after injection. The SSGC documented all hot nodes depicted by the CGC at 4 h after injection. <b>Conclusion:</b> The SSGC showed significant potential for the detection of sentinel lymph nodes in lymphoscintigraphy. The results of the studies suggested that the SSGC facilitates the exploration of hot nodes in sentinel lymph node biopsy.
A Cre-Expressing Cell Line and an E1/E2a Double-Deleted Virus for Preparation of Helper-Dependent Adenovirus Vector
Authors: Heshan Zhou, Tiejun Zhao, Lucio Pastore, Maged Nageh, Wendy Zheng, X.Mei Rao, Arthur L. Beaudet
Journal: Molecular Therapy (2001)

Adenoviral vectors are attractive for the delivery of transgenes into mammalian cells because of their efficient transduction, high titer, and stability. The major concerns with using E1-deleted adenoviral vectors in gene therapy are the pathogenic potential of the virus backbone and the leaky viral protein synthesis that leads to host immune responses and a short duration of transgene expression. Helper-dependent (HD) adenoviral vectors that are devoid of all viral protein-coding sequences have significantly increased the safety and reduced the immunogenicity of these vectors. Currently available HD vectors depend on an E1-deleted adenovirus as a helper to provide viral proteins in trans. As a consequence, contamination with helper virus cannot be avoided in the HD vector preparation though it can be decreased to 0.01% using a Cre/loxP mechanism. Since the presence of E1-deleted helper virus may have substantial unwanted effects, we have developed a new Cre-expressing cell line based on an E1- and E2a-complementing cell. This new cell line can efficiently cleave the packaging region in the helper virus genome. We have also developed an E1 and E2a double-deleted helper virus. By using the CreE cell with the helper virus deleted in both the E1 and the E2a genes it may be possible to further improve the safety of the vectors.
Orchestration of Iron Homeostasis
Authors: Robert E. Fleming, Bruce R. Bacon
Journal: New England Journal of Medicine (2005)

The number of newly identified genes participating in the regulation of iron homeostasis has increased at a remarkable pace. The characterization of these genes has led investigators to challenge previous models of the regulation of iron homeostasis in health and its dysregulation in disease. There is now substantial evidence that the liver plays a central role in determining how much iron is absorbed from the gut and in influencing the release of iron from sites of storage. The discovery of the iron regulatory hormone hepcidin has provided a cohesive theory to explain the pathophysiology of such common disorders as hereditary . . .
A comparison of the accuracy of peritoneoscopy and liver biopsy in the diagnosis of cirrhosis
Authors: M. Bruguera, J. M. Bordas, P. Mas, J. Rodes
Journal: Gut (1974)

The accuracy of peritoneoscopy and liver biopsy in the diagnosis of hepatic cirrhosis was compared in 473 consecutive patients submitted to both procedures. One hundred and fifty-two of them had cirrhosis diagnosed by one or both methods. There was 73% agreement between the two procedures. `Apparent9 false-negative results were 17·7% for peritoneoscopy and 9·3% for liver biopsy. The incidence of false-negative results in the diagnosis of cirrhosis can be reduced by combining both procedures.