tbuckley/cabot-search huggingface dataset:
Abstract 2424: Conditioned media enhances proliferation and mobility in triple negative breast cancer cells
Authors: KiTani A. Parker-Johnson, Letitia Yearby, Gabriel Beverly, Josiah Ochieng
Journal: Cancer Research (2012)

Abstract Background. Triple negative breast cancer (TNBC) is one of the most aggressive forms of breast cancer and disproportionally affects African American women. This subtype of breast cancer is characterized by the absence of the estrogen, progesterone, and HER2/neu receptors. Our research has provided insight on understanding that the microenvironment, specifically noncancerous cells, play in enhancing cancer progression and metastasis. However, the role of adjacent noncancerous cells in the metastatic phenomenon is poorly understood. Therefore, the aims of this project were: 1) to determine if conditioned media from MCF-10A cells would enhance proliferation of MDA-MB-231 and MDA-MB-468 cells and 2) to evaluate the ability of the effects of migration of the conditioned media on the MDA-MB-231 and MDA-MB-468 cells. Methods. Crystal violet and alamar blue dye exclusion assays were performed to measure proliferation in the different medias. The trans well migration assay was used to evaluate motility enhancement based on the media used, either conditioned media and the Cellometer was used to evaluate whether the MDA-MB-468 and MCF-10A cells shared the same microenvironment. Results. Conditioned media is made from base medium with a concentration of 1.0 g/L glucose with .5% FBS exposed to non-cancerous MCF-10A cells that have been driven into crisis. This conditioned media encouraged robust and continuous proliferation of the tumorigenic MDA-MB-231 and MDA-MB-468 cell lines. HGCM was extracted in our lab using sterile technique and reused to analyze the proliferative effects on cancerous and non-cancerous cells. MDA-MB-231 cells demonstrated significant proliferation when compared to the control. MDA-MB-468 cells demonstrated a similar response. The trans well migration assay demonstrated that the GFP labeled MDA-MB-468 cells had a higher percentage of motility than with MCF-10A cells in standard media. Conclusions: Taken together, these data indicate that factors secreted into the microenvironment from noncancerous cells may contribute to the mobility of TNBC cells and the conditioned media contains unknown factors that promote the cell mobility of the MDA-MB-231 and MDA-MB-468 cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2424. doi:1538-7445.AM2012-2424
Phospholipase D produces increased contractile force in rabbit ventricular muscle.
Authors: G A Langer, T L Rich
Journal: Circulation Research (1985)

Inclusion of phospholipase D (Streptomyces chromofuscus, 5 U/ml) in the medium perfusing rabbit papillary muscles increased peak force development by 78% and peak dF/dt by 89%. The maximal contractile response occurred 40-50 minutes after addition of the enzyme to the perfusate. As peak contractile response developed, aftercontractions appeared in most muscles. The inotropic response was slowly reversible, with disappearance of aftercontractions, upon discontinuation of the enzyme. Phospholipase D produces a specific increase of phosphatidic acid in the sarcolemma and, therefore, an increase in net anionic charge on the membrane. It has been shown previously that phospholipase D induces a large increase in sarcolemmal calcium binding. We propose, on the basis of the present study, that phospholipid-bound calcium may play a significant role in the control of contractile force in mammalian myocardium.
Determination of right atrial and right ventricular size by two-dimensional echocardiography.
Authors: W Bommer, L Weinert, A Neumann, J Neef, D T Mason, A DeMaria
Journal: Circulation (1979)

No data are available on determining right atrial and right ventricular size by two-dimensional echocardiography. We performed two-dimensional echocardiograms on eight human right-heart casts obtained at autopsy and on 50 patients who underwent complete left- and right-heart catheterization. Measurement of individual dimensions of the long and short axes of the right atrium and ventricle from right heart casts closely correlated with the volume of these structures as determined by water displacement. Further, individual dimensions by cross-sectional echo correlated well with actual casts dimensions. Subsequently, echocardiographic measurements of right atrial and ventricular long and short axes were obtained in the apical four-chambered view in a group of normals and compared with a group of patients with right ventricular volume overload states. Mean values for right atrial short-axis and long-axis measurements were greater in right ventricular volume overload patients than in normals: 6.5 +/- 0.3 vs 3.6 +/- 0.1 cm, and 6.0 +/- 0.3 vs 4.2 +/- 0.1 cm, respectively (both p less than 0.001). In addition, measurements of both individual dimensions as well as planed area of the right ventricle were greater in right ventricular volume overload patients than in normals: maximal short axis 6.1 +/- 0.3 vs 3.5 +/- 0.2 cm, mid-short axis 6.1 %/- 0.4 vs 2.8 +/- 0.2 cm, and area 40 +/- 2.6 vs 18 +/- 1.2 cm2 (all p less than 0.001). There were no differences in right ventricular long-axis measurement. Two-dimensional echocardiography provided better separation of normals from right ventricular volume overload patients than did M-mode techniques. Thus, two-dimensional echocardiography, with the apical four-chambered view, enables accurate visualization of the right atrium and ventricle in almost all patients. Futher, measurements of right atrial and right ventricular size by two-dimensional echocardiography readily distinguish normal patients from those with right ventricular volume overload.
Recurrence After Endoscopic Mucosal Resection for Superficial Esophageal Cancer
Authors: T. Nomura, N. Boku, A. Ohtsu, M. Muto, S. Matsumoto, H. Tajiri, S. Yoshida
Journal: Endoscopy (2000)

We present the results of endoscopic mucosal resection (EMR) for superficial esophageal cancer in patients treated at the National Cancer Center Hospital East since March 1993, and discuss the factors involved in local recurrence.The study consisted of 51 patients with a total of 57 superficial esophageal cancers which were treated by EMR at the National Cancer Center Hospital East between March 1993 and March 1998. EMR was performed with a two-channel fiberscope or with the assistance of the endoscopic esophageal mucosal resection tube. Follow-up examinations by means of endoscopy with iodine staining and biopsy were repeated every 3-6 months.A total of 19 patients had double cancers; 12 had head and neck cancers (HNC), six had stomach cancers, and one had lung cancer. The patients with HNC tended to have multiple iodine-unstained areas, and multiple cancers in the esophagus. Local recurrence was detected in two out of five patients (40%) with multiple esophageal cancers, and in two out of 46 patients (4%) with solitary cancer (P=0.0433). There was no difference in the rate of local recurrence between patients with HNC and those without HNC. Three out of four patients with recurrent cancers were given additional treatment, EMR for two and radiotherapy alone for one; no further recurrence occurred except in the patient who underwent radiotherapy alone.Multiplicity of cancer is a risk factor for local recurrence. Appropriate additional treatment should be indicated for recurrent lesions.
In vitro ischaemic preconditioning of isolated rabbit cardiomyocytes: effects of selective adenosine receptor blockade and calphostin C
Authors: Stephen Armstrong, Charles E Ganote
Journal: Cardiovascular Research (1995)

The aim was to determine if in vitro ischaemic preincubation can precondition cardiomyocytes and if the responses to adenosine receptor antagonists are similar to those previously determined during "metabolic" preconditioning with glucose deprivation or adenosine agonists.Isolated rabbit cardiomyocytes were preconditioned with 10 min of ischaemic preincubation, followed by a 30 min postincubation before the final sustained ischaemic period. The protein kinase C inhibitor calphostin C or the adenosine receptor antagonists 8-sulphophenyltheophylline (SPT), BW 1433U, and 1,3-dipropyl-8-cyclopentylxanthine (DPCPX) were added either during the preincubation or into the final ischaemic pellet. Adenosine deaminase (10 U.ml-1) was added during ischaemic preincubation. Rates of contracture and extent of injury were determined by sequential sampling and assessment of trypan blue permeability following 85 mOsM swelling.Myocytes were preconditioned by a 10 min in vitro ischaemic preincubation. Preincubation with 100 microM SPT or with adenosine deaminase, or addition of 200 nM calphostin C into the final ischaemic pellet did not alter rates of rigor contracture but nearly abolished protection. A significant degree of protection was maintained following ischaemic preincubation with the highly selective adenosine A1 receptor blocker DPCPX (10 microM), while the A1/A3 antagonist BW 1433U (1 microM) severely limited protection. SPT and BW 1433U added only into the final ischaemic pellet of preconditioned cells significantly blocked protection, while protection was maintained in the presence of DPCPX.Ischaemic preconditioning of cardiomyocytes is blocked by adenosine receptor antagonists known to bind to A3 receptors but not by DPCPX which has high affinity for A1 receptors, but little affinity for A3 receptors. Maintenance of protection during the final ischaemic phase has a similar receptor specificity. Blockade of protein kinase C activity abolishes protection. Ischaemic and metabolic preconditioning in vitro appear to occur through similar pathways.
Mouse allergen exposure, wheeze and atopy in the first seven years of life
Authors: W. Phipatanakul, J. C. Celedón, E. B. Hoffman, H. Abdulkerim, L. M. Ryan, D. R. Gold
Journal: Allergy (2008)

Little is known about mouse allergen exposure in home environments and the development of wheezing, asthma and atopy in childhood.
A Nowhere Near-Death Experience: Heavenly Choirs Interrupt Myelography
Authors: Francis O. Walker
Journal: JAMA (1989)

<h3>To the Editor.—</h3> Descriptions of the experience of surviving near-death events are entertaining,<sup>1</sup>but should be interpreted with caution. <h3>Report of a Case.—</h3> A 40-year-old woman with normal findings on physical examination except for breakaway weakness and nonanatomic sensory loss, underwent myelography for long-term lower back pain. Immediately after subarachnoid instillation of iohexol she became light-headed, then unresponsive, with labored breathing. Respiratory resuscitation was initiated by a radiologist, but when the code team arrived, her breathing, arterial blood values, and blood pressure were normal. They found fluctuating stiffness and weakness in her legs, normal reflexes, and defensive behavior. Although alert and talkative, she refused to cooperate with sensory testing or to answer questions about her paraparesis. Unexpectedly, labored respirations and unresponsiveness recurred. Intubation was attempted, which provoked a prompt return of consciousness and normal breathing; leg function returned to normal. The spell was judged to be a conversion reaction
"INTERNATIONAL HEALTH INSTITUTE"
Authors: J. Severy Hibben
Journal: JAMA (1927)

To the Editor:  —I was much surprised and humiliated in looking throughThe Journal, April 30, to find on page 1435, under the Propaganda for Reform, your investigation of the International Health Institute, with my name listed as on the advisory board.  It is true that I did send in my name, signifying my willingness to be examiner for them, but of course had no idea that it was any such a layout asThe Journalhas proved it to be. I took this step without investigating the matter at all for which I am certainly sorry, and I am enclosing a letter which I have written to the International Health Institute asking them to take my name off the reference board.
Mentally disordered or lacking capacity? Lessons for management of serious deliberate self harm
Authors: A. S. David, M. Hotopf, P. Moran, G. Owen, G. Szmukler, G. Richardson
Journal: BMJ (2010)

Most post transplantation lymphoproliferative disorders (PTLDs) are Epstein-Barr virus (EBV) associated B cell proliferations. We report a case of aggressive anaplastic large cell lymphoma expressing the anaplastic lymphoma kinase (ALK) protein in a 58 year old man who had previously undergone liver transplantation. A definite diagnosis was not possible on histopathological examination. Immunostaining clearly showed a predominant population of small irregular lymphocytes, admixed with large cells strongly positive for CD30, epithelial membrane antigen, and the ALK protein. Neoplastic cells were of the T/cytotoxic phenotype. In situ hybridisation with EBV encoded early RNA probes showed only a few scattered positive non-neoplastic small lymphocytes. Polymerase chain reaction analysis of immunoglobulin and T cell receptor rearrangements was negative. The NPM–ALK fusion transcript associated with the t(2;5) translocation was detected by reverse transcription polymerase chain reaction. A review of the literature revealed 76 cases of T cell PTLD, showing a broad spectrum of morphological features and clinical behaviour. Most of these cases were EBV negative (61 of 76) and occurred after renal transplantation (48 of 76). To our knowledge, this is the first case of ALK positive lymphoma occurring in the setting of organ transplantation. This observation stresses the need for accurate immunostaining for diagnosing this rare, apparently aggressive, lymphoma in immunosuppressed patients.
Streptococcal infection may make psoriasis worse but do antibiotics help?
Authors: C.M. Owen, R.J.G. Chalmers, C.E.M. Griffiths
Journal: British Journal of Dermatology (2004)

Journal Article Streptococcal infection may make psoriasis worse but do antibiotics help? Get access C.M. Owen, C.M. Owen Department of Dermatology, Burnley General Hospital, Burnley, and *Dermatology Centre, University of Manchester, Hope Hospital, Salford, Manchester, U.K. E‐mail: carolineo@doctors.org.uk Search for other works by this author on: Oxford Academic Google Scholar R.J.G. Chalmers, R.J.G. Chalmers Department of Dermatology, Burnley General Hospital, Burnley, and *Dermatology Centre, University of Manchester, Hope Hospital, Salford, Manchester, U.K. E‐mail: carolineo@doctors.org.uk Search for other works by this author on: Oxford Academic Google Scholar C.E.M. Griffiths C.E.M. Griffiths Department of Dermatology, Burnley General Hospital, Burnley, and *Dermatology Centre, University of Manchester, Hope Hospital, Salford, Manchester, U.K. E‐mail: carolineo@doctors.org.uk Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 151, Issue 1, 1 July 2004, Pages 244–245, https://doi.org/10.1111/j.1365-2133.2004.06059.x Published: 01 July 2004
Should life be longer?
Authors: Claus A Pierach
Journal: BMJ (2004)

whole will bear an opportunity cost when resources are not allocated so that benefits will be maximised to all.Thus it could be argued that at a certain level of response, the costs of minimising the risk of food anaphylaxis will outweigh the likely benefits.However, the point at which such a threshold lies depends on how the consequences are viewed, and the societal values inherent in such evaluations.Children occupy a special place in modern society, such that their safety and access to education and health care are viewed as priorities.Accordingly, the public has much sympathy for attempts to reduce risks to children and with parental predicaments created by such attempts.An argument can therefore be made on sociocultural grounds that we all gain from efforts to prevent a child's death, justifying the liberal provision of adrenaline autoinjectors and public health measures to restrict exposure to peanuts.A precautionary approach could then be acceptable, even if costs are borne by many and the benefits are unquantifiable.
Differentiation-associated localization of small prolne-rich rotein in normal and diseased human skin
Authors: H. KOIZUMI, T. KARTASOVA, H. TANAKA, A. OHKAWARA, T. KUROKI
Journal: British Journal of Dermatology (1996)

The expression of SPRR (small proline‐rich protein) was investigated in normal human skin and in diseased skin from patients with psoriasis, squamous cell carcinoma, basal cell epithelioma. Naevus pigmentosus, ichthyosis vulgaris and several inflammatory skin diseases, by immunohistochemical staining. A polyclonal antibody was raised against a synthetic peptide for a C‐terminal common region for SPRR l and SPRR 3. In immunoblot analysis, a positive band of 18kDa was detected, which showed the presence of SPRR l in human epidermal keratinocytes. In normal epidermis, positive staining for SPRK was observed in keratinocytes in the granular layer and the uppermost or two spinous cell layers, with no staining of the other spinous or basal layers. The staining was obvious at the cell periphery, weak at the cytoplasm, and absent in the nucleus. Staining was observed in several outer layers of the follicular infundibulum to the isthmus. No staining was detected in the inner root sheath of the hair follicles, hair matrix, sebaceous gland, eccrine gland, eccrine duct, melanocytes. Langerhans cells or fibroblasts. The arrectores pilorum, striated muscles, muscle layers of vessels, and myoepithelia of eccrine gland, were weakly stained. In psoriatic skin, stained keratinocytes were distributed in the spinous cell layers except for the basal layer, in ichthyosis vulgaris. SPRR was barely expressed in the uppermost living cell layers of the epidermis in epidermolytic hyperkeratosis. degenerated squamous cells widely expressed SPRR. In Darier's disease, dyskeratolic cells were clearly stained. In squamous cell carcinoma, staining was observed in keratotic cells around horny pearls. In basal cell epithelioma, naevus pigmentosus, and malignant melanoma, the tumour cells or naevus cells were not stained. The distribution of SPRR was similar to that of involucrin in normal and several diseased skin, except for ichthyosis vulgaris. We conclude that SPRR is expressed in close association with epidermal differentiation in normal skin and skin diseases. The alteration of the expression of the proteins correlated to terminal differentiation, and differs from disease to disease.
COX-2-dependent PGE2 acts as a growth factor in mycosis fungoides (MF)
Authors: K L M Kopp, C S Kauczok, B Lauenborg, T Krejsgaard, K W Eriksen, Q Zhang, M A Wasik, C Geisler, E Ralfkiaer, J C Becker, N Ødum, A Woetmann
Journal: Leukemia (2010)
A Medical Student on Call
Authors: Adria Burrows
Journal: JAMA (1983)

It was the first night on call of my medicine rotation and my intern handed me a couple of tubes and told me to draw blood from Mrs Matthews, a patient with advanced glioblastoma multiforme. Her room was dim, lighted only by a streetlamp outside. I could see her shaved head and obese body, a huge mound under the sheets. Her wrists were tied to the bed at her sides. Snoring loudly, mouth half open, she lay on her back, and I shook her shoulder gently and called out her name, but the snores continued. Nevertheless, I talked to her, mostly because I was nervous on the second day of my new rotation and I felt like talking to someone. "Hello, Mrs Matthews," I said. "I just want to get some blood from you. This will just take a few minutes and then you can go back to sleep." The
Effect of circulation time on plasma high density lipoprotein cholesterol in rats
Authors: T. ISHIZUKA
Journal: Cardiovascular Research (1988)

Journal Article Effect of circulation time on plasma high density lipoprotein cholesterol in rats Get access TAKEHIKO ISHIZUKA TAKEHIKO ISHIZUKA From the Department of the First Pathology, Dokkyo University School of Medicine, Mibu, Tochigi, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 22, Issue 5, May 1988, Pages 368–371, https://doi.org/10.1093/cvr/22.5.368 Published: 01 May 1988 Article history Received: 28 July 1987 Accepted: 13 January 1988 Published: 01 May 1988
Abstract 4911: NSCLC cells demonstrate differential mode of cell death in response to the combined treatment of radiation and a DNA-PKcs inhibitor
Authors: Yu Lan, Zengfu Shang, Feng-Ming Hsu, Vasu Tumati, Benjamin P. Chen, Debabrata Saha
Journal: Cancer Research (2014)

Abstract Introduction: Lung cancers demonstrate the highest number of p53 mutations of any cancer with 70% of small cell lung cancers and 33% of adenocarcinomas containing a mutation. Loss of p53 function has been correlated with therapeutic resistance and poor clinical outcomes. Therefore, developing therapeutic strategies that specifically target p53 loss-of-function mutants is of the utmost importance. The purpose of this investigation is to explore whether p53 status has impact on the radio-sensitizing effect of a DNA-PKcs inhibitor (NU7441). Methods: Clonogenic surviving fraction analysis, DNA double strand break repair kinetics, cell cycle analysis, immunofluorescence, immunoblot, apoptosis and autophagy assays were used to investigate the mechanism of radiosensitization of NU7441 in NSCLC cell lines (A549, H1299, and H460) with differential levels of p53 expression. Results: NU7441 substantially inhibited the repair of IR-induced DNA double strand break (DSB) in all three cell lines. Clonogenic surviving fraction analysis reveals that NU7441 is a better radio-sensitizer in p53-deficient cells (H1299) when compared to p53-wild type cells (A549, H460). Combination of ionizing radiation (IR) and NU7441 leads to a more robust G2 arrest in A549 and H460 cells when compared to radiation treatment only. However, NU7441 and radiation preferentially induced mitotic arrest in H1299 consequently leading to a significant increase in mitotic catastrophe. Furthermore, NU7441 led to an increase in apoptosis rather than autophagy as the predominant cell death pathway in p53-deficient cells. Moreover, knockdown of p53 using siRNA sensitized A549 to NU7441+IR treatment. Conclusion: Our study clearly shows that NU7441, a novel DNA-PKcs inhibitor, is a potent radio-sensitizer in p53-dificient lung cancer cells and highlights the promise of NU7441 in targeted cancer treatment. Citation Format: Yu Lan, Zengfu Shang, Feng-Ming Hsu, Vasu Tumati, Benjamin P. Chen, Debabrata Saha. NSCLC cells demonstrate differential mode of cell death in response to the combined treatment of radiation and a DNA-PKcs inhibitor. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4911. doi:10.1158/1538-7445.AM2014-4911
Abortion Law Reform
Authors: Fred Rosner
Journal: JAMA (1971)

<h3>To the Editor.—</h3> I must assume that an oversight is responsible for the omission of dates for the recently amended abortion laws of Hawaii ( 1970), Oregon (1969), South Carolina (1970), and Virginia (1970) in the table (<b>214:</b>2238, 1970). In the following week's issue, (<b>214:</b>2405), it is hard to understand why Illinois and Texas are not mentioned in the discussion of the constitutional questions relating to abortion laws. A three-judge Federal court ruled Texas' abortion law unconstitutional in that it is too "vague and overbroad" and that the "fundamental right of single women and married persons to choose whether to have children" is protected by the Ninth Amendment of the Constitution (<i>AMA News</i>, Aug 10, 1970 p 13). Similarly, in Chicago, a criminal court judge ruled that the Illinois abortion statute is unconstitutional because it is vague and "infringes upon a woman's right to control her own body" (<i>AMA</i>