tbuckley/cabot-search huggingface dataset:
Abstract 5533: Imaging stromal recruitment during liver metastasis formation in the GFP-MUP-uPA mouse
Authors: Atsushi Suetsugu, Hisanobu Ogata, Hisataka Moriwaki, Shigetoyo Saji, Michael Karin, Robert M. Hoffman
Journal: Cancer Research (2012)

Abstract Major urinary protein-urokinase type plasminogen activator (MUP-uPA) transgenic mice develop liver disease resembling that described for albumin promoter-uPA transgenic mice. We have developed the GFP-MUP-uPA mice, by crossing MUP-uPA mouse with transgenic C57/BL6 mouse expressing GFP. Hepatocyte fluorescence in this model is relatively weak. In contrast, GFP fluorescence of non-parenchymal cells such as stellate cells, Kupper cells and endotherial cells is very bright green more than parenchymal cells enabling the portal vein and sinusoid structure to be clearly visualized Huh7 liver cancer cells expressing red fluorescent protein (RFP) were injected in the spleen of MUP-uPA transgenic mice. By day 7, the Huh7-RFP celsl were visualized in the liver with the Olympus FV1000 conforcal microscope. The hepatoma cells grew around the sinusoids of the host liver. The liver-metastatic Huh7-RFP cells recruited bright GFP-expressing non-parenchymal cells. This model enables imaging the effects of uPA on liver metastasis and stromal recruitment enabling the metastasis to progress. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5533. doi:1538-7445.AM2012-5533
Primary Acute Lymphoblastic Leukemia Cells Use a Novel Promoter and 5′Noncoding Exon for the Human Reduced Folate Carrier That Encodes a Modified Carrier Translated from an Upstream Translational Start
Authors: Robin M. Flatley, Scott G. Payton, Jeffrey W. Taub, Larry H. Matherly
Journal: Clinical Cancer Research (2004)

Abstract The human reduced folate carrier (hRFC) is reported to be regulated by up to seven alternatively spliced noncoding exons (A1, A2, A, B, C, D, and E). Noncoding exon and promoter usage was analyzed in RNAs from 27 childhood acute lymphoblastic leukemia (ALL) specimens by real-time PCR and/or 5′ rapid amplification of cDNA ends (5′ RACE) assay. By real-time PCR, total hRFC transcripts in ALL spanned a 289-fold range. Over 90% of hRFC transcripts were transcribed with A1, A2, and B 5′ untranslated regions (UTRs). Analysis of 5′ RACE clones showed that the A1 + A2 5′UTRs contained A1 sequence alone or a fusion of A1 and A2, implying the existence of a single, alternatively spliced 1021-bp A1/A2 noncoding region. High frequency sequence polymorphisms (AGG deletion, C/T transition) identified in the A1/A2 region by 5′RACE were confirmed in normal DNAs. By reporter assays in HepG2 hepatoma and Jurkat leukemia cells, A1/A2 promoter activity was localized to a 134-bp minimal region. Translation from an upstream AUG in the A1/A2 noncoding region in-frame with the normal translation start resulted in synthesis of a larger (∼7 kDa) hRFC protein with transport properties altered from those for wild-type hRFC. Although there was no effect on transcript or protein stabilities, in vitro translation from A1/A2 transcripts was decreased compared with those with the B 5′UTR. Our results document the importance of the hRFC A1/A2 upstream region in childhood ALL and an intricate transcriptional and posttranscriptional regulation of hRFC-A1/A2 mRNAs. Furthermore, they suggest that use of the A1/A2 5′UTR may confer a transport phenotype distinct from the other 5′UTRs due to altered translation efficiency and transport properties.
The Lazarus Complex Revisited
Authors: THOMAS P. HACKETT
Journal: Annals of Internal Medicine (1972)

Editorials1 January 1972The Lazarus Complex RevisitedTHOMAS P. HACKETT, M.D.THOMAS P. HACKETT, M.D.Author, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-76-1-135_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe cycle of death and resurrection has intrigued men for centuries, but in our time social scientists have shown remarkably little interest in those few individuals who have gone through that perilous transition. The survivors of cardiac arrest (probably more than any other single group of patients) qualify as latter-day versions of Lazarus. In contrast to their biblical forebear who apparently suffered no ill effects as the result of his return from the dead, patients successfully saved from heart stop-page, as reported by Druss and Kornfeld (1), have a more hazardous course. These two psychiatric investigators published the first study...References1. DRUSS R and KORNFELD D: Survivors of cardiac arrest: a psychiatric study. JAMA 201:296, 1967 CrossrefGoogle Scholar2. LIFTON R: Death in Life. New York, Random House, 1967 Google Scholar3. DOBSON M, TATTERSFIELD A, ADLER M, and MCNICOL M: Attitudes in long-term adjustment of patients surviving cardiac arrest. Br Med J 3:207-212, 1971 CrossrefMedlineGoogle Scholar4. HACKETT T, CASSEM N, and WISHNIE H: The coronary care unit: an appraisal of its psychological hazards. N Engl J Med 279:1365-1370, 1968 CrossrefMedlineGoogle Scholar5. FEIFEL H: The Meaning of Death. New York, McGraw-Hill Book Co., Inc., 1959 Google Scholar6. CASSEM N, WISHNIE H, and HACKETT T: How coronary patients respond to last rites. Postgrad Med 45:147-152, 1969 CrossrefMedlineGoogle Scholar7. LAWRIE D: Long term survival after ventricular fibrillation complicating acute myocardial infarction. Lancet 2:1085-1087, 1969 CrossrefMedlineGoogle Scholar8. MCNAMEE B, ROBINSON T, and ADGEY A: Long term prognosis following ventricular fibrillation in acute ischaemic heart disease. Br Med J 4:204-206, 1970 CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: THOMAS P. HACKETT, M.D.Affiliations: Massachusetts General Hospital Boston, Mass. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byLazarus-PhänomenIntraoperatives „Lazarus-Phänomen“?The Lazarus phenomenonNeurology in Holy ScriptureUnexpected return of spontaneous circulation after cessation of resuscitation (Lazarus phenomenon)Near death experiences and death-related visions in children: Implications for the clinicianLaboratory Evaluation of Newly Admitted Psychiatric PatientsAttitude Change Following Near-Death ExperiencesThe Gift of Life: A Review of the Psychological Aspects of Kidney TransplantationSurvivors of Cardiac Arrest 1 January 1972Volume 76, Issue 1Page: 135-137KeywordsHeartScientists ePublished: 1 December 2008 Issue Published: 1 January 1972 PDF downloadLoading ...
Hazards of fiberoptic bronchoscopy.
Authors: R Stalker, R L Ward
Journal: BMJ (1979)

patients was the thrombus confined to the calf.This is at variance with a large study,' in which thrombus was confined to the calf in 4900 of 493 legs containing thrombus.To say that "there was a general reluctance on the part of individual doctors to perform phlebography on patients with negative 99mTc-red-cell scans" merely emphasises the bias of investigation in favour of their own technique.Secondly, this technique apparently lacks the facility of radionuclide venography, its nearest rival in providing a simultaneous lung scan.Finally, the acid test of all new diagnostic procedures is whether they may be applied in the district general hospital as readily as the teaching hospital.Conventional phlebography fulfils this criterion.We remain unconvinced that this new technique can alter the situation in any way, being more demanding in time, expertise, skilled man- power, and expensive equipment.
Feedback regulation of bile acid synthesis in the rat by dietary vs. Intravenous cholate or taurocholate
Authors: Eduard F. Stange, Jürgen Scheibner, Christine Lutz, Hans Ditschuneit
Journal: Hepatology (1988)

The regulation of bile acid synthesis was studied (i) in intact or colectomized rats receiving cholate or taurocholate as a dietary supplement and (ii) in experiments using chow-fed animals with a graded intravenous or intraduodenal taurocholate infusion. After the 2-week diet period a bile fistula was established and rates of taurocholate, tauromuricholate and taurochenodeoxy-cholate secretion were quantitated by high-performance liquid chromatography. During the infusion experiments taurocholate production was calculated from the difference in specific activity of [ 14 C]taurocholate between infusate and bile, whereas tauromuricholate and taurochenodeoxycholate synthesis was derived directly from their secretion rates after pool depletion. Both the 0.5% cholate and taurocholate diet suppressed tauromuricholate and taurochenodeoxycholate secretion nearly totally, but only cholate led to a prolonged inhibition taurocholate synthesis. The diets stimulated total bile acid secretion and expanded the total bile acid pool size 2- to 3-fold, but they also prompted a dramatic increase in the biliary secretion of taurodeoxycholate. In contrast, colectomized animals did not secrete taurodeoxycholate following the cholate diet and, despite a comparable increase in bile acid pool size, tauromuricholate and taurochenodeoxycholate secretion was inhibited to a lesser extent. In addition, the rate of bile acid secretion and synthesis was significantly enhanced when compared to that of intact rats. To determine whether taurocholate affected bile acid synthesis directly, the bile acid was infused intravenously or intraduodenally at varying rates up to 300 μmoles per kg per hr for 54 hr, i.e. a rate exceeding normal total bile acid secretion in these acute bile fistula animals nearly 3-fold. However, there was no decrease in both tauromuricholate, and taurocholate synthesis and taurochenodeoxycholate was lowered only by the highest dose of taurocholate and only during the derepressed phase. When a taurocholate infusion was given to rats prefed 0.5% cholate there was no evidence for an enhanced feedback regulation by a preceding pool expansion. It is suggested that the suppressive effect of dietary cholate or taurocholate or bile acid formation may not be mediated by the primary bile acid directly, but rather by secondary bile acids formed in the colon.
Pathophysiological significance of coronary collaterals for preservation of the myocardium during coronary occlusion and reperfusion in anaesthetised dogs
Authors: M. TANABE, S. FUJIWARA, N. OHTA, N. SHIMAMOTO, M. HIRATA
Journal: Cardiovascular Research (1980)

The significance of pre-existing coronary collaterals for preservation of the myocardium during coronary occlusion and reperfusion was studied in anaesthetised open-chest dogs. The extent of myocardial ischaemic damage was assessed by the accumulation of calcium in the central zone of the ischaemic area and the size of myocardial infarct by the p-nitroblue tetrazolium staining method. Myocardial creatine kinase release was considered as an index reflecting both the extent of ischaemic myocardial damage and the size of the infarct. Reperfusion with the arterial blood of a main branch of the left coronary artery following the 60 min occlusion aggravated the ischaemic myocardial damage, as evidenced by exaggerated cardiac irregularity, myocardial calcium accumulation, and creatine kinase release. The myocardial calcium content and the logarithm of the creatine kinase release during reperfusion were linearly related to the infarct size. These parameters of myocardial damage were inversely related to the retrograde blood flow through the peripheral end of the occluded artery or the ratio of the retrograde-to-orthograde flow, indicating that the pre-existing collaterals influence preservation of the ischaemic myocardium. Thus, it is suggested that the collateral flow available during an ischaemic episode determines the viability of the myocardial cells during ischaemia, and in turn during reperfusion.
Prognostic Validation of the Body Mass Index, Airflow Obstruction, Dyspnea, and Exercise Capacity (BODE) Index in Inoperable Non–Small-Cell Lung Cancer
Authors: Linda Denehy, Whitney E. Hornsby, James E. Herndon, Samantha Thomas, Neal E. Ready, Catherine L. Granger, Lauren Valera, Aarti A. Kenjale, Neil D. Eves, Lee W. Jones
Journal: Journal of Thoracic Oncology (2013)
Diurnal Variation in Glucose Tolerance: Associated Changes in Plasma Insulin, Growth Hormone, and Non-esterified
Authors: P. Z. Zimmet, J. R. Wall, R. Rome, L. Stimmler, R. J. Jarrett
Journal: BMJ (1974)

Oral glucose tolerance tests were performed in the morning and afternoon of separate days on 31 people derived from a normal population sample. Blood sugar levels were higher in the afternoon test from and including 60 minutes after the glucose load. The degree of diurnal variation was similar in men and women, but greater in the older half of the group. It was negatively correlated with the degree of obesity. The plasma insulin response was less at the 30 minute time point in the afternoon, but significantly exceeded the morning values at 120 and 150 minutes after the glucose load. Growth hormone levels were similar in morning and afternoon tests. Fasting non-esterified fatty acid levels were significantly higher before the afternoon test. The relatively impaired glucose tolerance in the afternoon is associated with a delayed insulin response to the glucose load. This seems unlikely to be the sole explanation, however, and increased non-esterified fatty acid metabolism with a consequent decrease in glucose disposal may also contribute.
Mesenchymal stem cells inhibit natural killer–cell proliferation, cytotoxicity, and cytokine production: role of indoleamine 2,3-dioxygenase and prostaglandin E2
Authors: Grazia Maria Spaggiari, Andrea Capobianco, Heba Abdelrazik, Flavio Becchetti, Maria Cristina Mingari, Lorenzo Moretta
Journal: Blood (2007)
Interventions for Autism
Authors: Alan R. Gill
Journal: JAMA (2001)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Electrical potentials in epithelial neoplasms
Authors: N. Melczer, J. Kiss
Journal: British Journal of Dermatology (1977)

Journal Article Electrical potentials in epithelial neoplasms Get access N. Melczer, N. Melczer Department of Dermatology, University of Pécs and United Hospitals of Municipal Council, Esztergom, Hungary Search for other works by this author on: Oxford Academic Google Scholar J. Kiss J. Kiss Department of Dermatology, University of Pécs and United Hospitals of Municipal Council, Esztergom, Hungary Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 96, Issue 5, 1 May 1977, Pages 572–573, https://doi.org/10.1111/j.1365-2133.1977.tb07166.x Published: 01 May 1977
Prevention of bleeds in hemophilia patients with inhibitors: Emerging data and clinical direction
Authors: Cindy A. Leissinger
Journal: American Journal of Hematology (2004)

In patients with hemophilia, the development of high-responding inhibitors to factor VIII prevents adequate replacement therapy and results in increased risk of serious bleeding episodes, poor control of joint bleeding, and progressive, debilitating joint disease. Immune tolerance therapy can eradicate inhibitors, but it is not uniformly successful. Emerging data suggest that prophylaxis using activated prothrombin complex concentrates may be effective and safe in reducing the incidence of joint bleeding during immune tolerance therapy and for patients in whom immune tolerance induction fails. However, only controlled clinical trials will ultimately demonstrate whether prophylaxis can prevent joint bleeding and damage, and improve quality of life in patients with inhibitors.
Public Health Law in a New Century
Authors: Lawrence O. Gostin
Journal: JAMA (2000)

Public health interventions need justification because they intrude on individual rights and incur economic costs. Coercive interventions can be justified in only 3 cases: to avert a risk of serious harm to other persons, to protect the welfare of incompetent persons, and, most controversially, to prevent a risk to the person himself/herself. This article proposes a systematic evaluation of public health regulation. The article recommends that public health authorities should bear the burden of justification and, therefore, should demonstrate (1) a significant risk based on scientific evidence; (2) the intervention's effectiveness by showing a reasonable fit between means and ends; (3) that economic costs are reasonable; (4) that human rights burdens are reasonable; and (5) that benefits, costs, and burdens are fairly distributed. The 3 articles in this series have sought to provide a fuller understanding of the varied ways in which law can advance the public's health. Public health law should be seen broadly as the government's power and responsibility to ensure the conditions for the population's health. As such, public health law has transcending importance in how we think about government, politics, and policy. JAMA. 2000.
When 7 transmembrane receptors are not G protein-coupled receptors
Authors: Keshava Rajagopal, Robert J. Lefkowitz, Howard A. Rockman
Journal: Journal of Clinical Investigation (2005)

Classically, 7 transmembrane receptors transduce extracellular signals by coupling to heterotrimeric G proteins, although recent in vitro studies have clearly demonstrated that they can also signal via G protein-independent mechanisms. However, the physiologic consequences of this unconventional signaling, particularly in vivo, have not been explored. In this issue of the JCI, Zhai et al. demonstrate in vivo effects of G protein-independent signaling by the angiotensin II type 1 receptor (AT1R) (see the related article beginning on page 3045). In studies of the mouse heart, they compare the physiologic and biochemical consequences of transgenic cardiac-specific overexpression of a mutant AT1R incapable of G protein coupling with those of a wild-type receptor. Their results not only provide the first glimpse of the physiologic effects of this newly appreciated mode of signaling but also provide important and previously unappreciated clues as to the underlying molecular mechanisms.
"Give-Away Journals"
Authors: Herbert Silver
Journal: JAMA (1972)

To the Editor.—  I am frankly quite tired of the indignant physician who feels it is his duty to protect others from give-aways. Perhaps, if these physicians spent more time reading and less time writing to editors they would soon realize that give-aways contain a wealth of information. This enables those of us who are interested in doing so to read about concepts, ideas, and findings which are outside our specific field of interest and may not be presented in the numerous  we read. It is through this type of material that I learn of activities at those professional meetings I was unable to attend and I also become acquainted with work reported in  I do not routinely read.  Although Greenwald ( 217 :1705, 1971) and Silverman ( 217 :1574, 1971) found it so important that giveaway medical journals be done away with, I hope that they read the excellent
Stimulation of Fc gamma RIIIA results in phospholipase C-gamma 1 tyrosine phosphorylation and p56lck activation.
Authors: L Azzoni, M Kamoun, T W Salcedo, P Kanakaraj, B Perussia
Journal: The Journal of Experimental Medicine (1992)

Binding of ligand to the alpha subunit of Fc gamma RIIIA(CD16), expressed at the natural killer (NK) cell membrane in association with homo or heterodimers of proteins of the zeta family, results in phosphorylation of several proteins on tyrosine residues. We have analyzed the role of protein tyrosine phosphorylation in the regulation of molecular events induced upon stimulation of Fc gamma RIIIA in NK cells and in T cells expressing the Fc gamma RIII alpha chain in association with endogenous zeta 2 homodimers and devoid of other (CD3, CD2) transducing molecules. Our data indicate that treatment of these cells with protein tyrosine kinase inhibitors prevents not only Fc gamma RIIIA-induced protein tyrosine phosphorylation but also phosphatidylinositol 4,5 diphosphate hydrolysis and increased intracellular Ca2+ concentration, indicating a primary role of tyrosine kinase(s) in the induction of these early activation events. Occupancy of Fc gamma RIIIA by ligand results in phospholipase C (PLC)-gamma 1 tyrosine phosphorylation in NK cells and in Fc gamma RIIIA-transfected CD3-/CD2- T cells, and induces functional activation of p56lck in Fc gamma RIIIA alpha/zeta 2-transfected T cells, suggesting the possibility that the receptor-induced PLC-gamma 1 activation occurs upon phosphorylation of its tyrosine residues mediated by this kinase and is, at least in part, responsible for the signal transduction mediated via CD16 upon ligand binding.
Metabolic Responses to Oral Glucose in the Kalahari Bushmen
Authors: B. I. Joffe, W. P. U. Jackson, M. E. Thomas, M. G. Toyer, P. Keller, B. L. Pimstone, R. Zamit
Journal: BMJ (1971)

The plasma glucose, immunoreactive insulin, and growth hormone levels after a 50-g oral glucose load have been measured in 15 adult Bushmen subjects living in the Kalahari region of Southern Africa. Compared with 10 non-obese white controls, they showed relative glucose intolerance and significantly impaired insulin secretion. Growth hormone responses showed no significant differences between the two groups. Factors such as inadequate or unusual nutrition and stress do not appear to account completely for the abnormalities in carbohydrate metabolism observed in the Bushmen. Of interest are the clinical and hormonal similarities that seem to exist between the Bushmen and the Central African Pygmies.
A CASE OF EXCISION OF THE CERVICAL PORTION OF THE ESOPHAGUS.
Authors: WALLACE I. TERRY
Journal: JAMA (1905)

<h3>History.</h3> —The patient, M. K., aged 44 years, is a native of California and a farmer by occupation. Two years ago he was referred to me by Dr. George H. Powers, of San Francisco, on account of suspected malignant tumor of the larynx. A laryngo-fissure was done and the masses which sprang from the cords were removed, together with much of the mucous membrane of the larynx. The tumor was found to be an epithelioma. Four months later a recurrence necessitated another laryngo-fissure, at which time the wound was left wide open and<i>x</i>-ray treatment of the interior of the larynx was instituted. Despite this, the growth recurred, and in September, 1903, a total laryngectomy was done and the stump of the trachea sutured to the skin just above the sternum. Again, after five months, i. e., in February, 1904, a mass appeared in the scar and was excised.
A Post-Hoc Analysis Of Asthma Control And Lung Function Following Treatment With Ciclesonide 80 μg HFA-MDI Twice Daily In Subjects With Mild-to-Moderate Persistent Asthma Previously Receiving Low Dose Fluticasone Propionate/Salmeterol
Authors: B. Prenner, E.O. Meltzer, S.Y. Desai, J. Karafilidis, J. Walsh
Journal: Journal of Allergy and Clinical Immunology (2011)