tbuckley/cabot-search huggingface dataset:
1053. Novel Exogenously Regulated Stem Cell–Mediated Gene Therapy Platform for Spinal Fusion
Authors: Gadi Pelled, Amir Hasharoni, Yoram Zilberman, Gadi Turgeman, Gregory A. Helm, Meir Libergall, Dan Gazit
Journal: Molecular Therapy (2005)

Cell-mediated gene therapy involving mesenchymal stem cells (MSCs) and bone morphogenetic protein (BMP) genes can provide a local supply of precursor cells and osteoinductive molecules that may promote bone formation. Spinal fusion has become a popular surgical technique for different pathological spinal conditions. We hypothesized that MSCs that overexpress the human (h)BMP-2 gene under the tetracycline (Tet)-controlled expression system (Tet-Off) can induce regulated bone formation leading to spinal fusion. We used a Tet-Off system based on genetically engineered MSCs containing an inducible hBMP-2 expression vector (ptTATop-BMP2) that had a bidirectional promoter (TATA sequence) utilizing elements of the tetracycline regulatory system. The system was regulated by the administration of doxycycline (Dox), a tetracycline analogue. In C3H/HeN mice 2 |[times]| 10^6 genetically engineered MSCs were injected into the paravertebral muscle of the lumbar spine (L2|[ndash]|6). Ten animals served as a negative control group (Dox was added to their drinking water) and 20 animals constituted the experimental group (Dox was supplied for different time periods leading to different duration of hBMP-2 expression). A longitudinal quantitative analysis of vertebrae fusion was performed using micro|[ndash]|computerized tomography (|[mu]|CT) to observe bone formation. In addition, engineered MSCs were injected in a similar manner to transgenic mice expressing the luciferase gene under human osteocalcin promoter. In these mice measuring the luciferase signal using a bioluminescence imaging system can noninvasively and quantitatively monitor osteogenic activity. From Week 1 postinjection the |[mu]|CT analysis demonstrated extensive bone formation leading to vertebrae fusion (Fig. 1A). No bone formation was observed in the control animals, in which hBMP-2 was inhibited. A minimum of 7 days of hBMP-2 expression was required to induce bone formation (Fig. 1B). Detection of luciferase gene expression in the transgenic mice 2 and 4 weeks postinjection indicated an increase in the osteogenic activity in vivo, which could be attributed to the paracrine effect of the engineered MSCs (Fig. 2). Our data demonstrate a regulated system for the induction of spinal fusion. We conclude that exogenously controlled gene therapy for spinal fusion can be achieved using the Tet-regulated hBMP-2 system.
Impact of stent length on restenosis rate in small vessels
Authors: Konstantinos Toutouzas, Goran Stankovic, Takuro Takagi, Vassilis Spanos, Carlo Di Mario, Remo Albiero, Nicola Corvaja, Alaide Chieffo, Ginevra Sivieri, Antonio Colombo
Journal: Journal of the American College of Cardiology (2002)
TNF-α Mediates PKR-Dependent Memory Impairment and Brain IRS-1 Inhibition Induced by Alzheimer’s β-Amyloid Oligomers in Mice and Monkeys
Authors: Mychael V. Lourenco, Julia R. Clarke, Rudimar L. Frozza, Theresa R. Bomfim, Letícia Forny-Germano, André F. Batista, Luciana B. Sathler, Jordano Brito-Moreira, Olavo B. Amaral, Cesar A. Silva, Léo Freitas-Correa, Sheila Espírito-Santo, Paula Campello-Costa, Jean-Christophe Houzel, William L. Klein, Christian Holscher, José B. Carvalheira, Aristobolo M. Silva, Lício A. Velloso, Douglas P. Munoz, Sergio T. Ferreira, Fernanda G. De Felice
Journal: Cell Metabolism (2013)
Abstract LB-10: Distribution and potency of anti-proliferative arachidonic acid metabolites
Authors: Sonia D. Revello, Nathaniel W. Snyder, Xiaojing Liu, Suhong Zhang, Jasbir S. Arora, Ian A. Blair
Journal: Cancer Research (2012)

Abstract Eicosanoids participate in the dysregulation of cell proliferation during tumorigenesis. The up-regulation of cyclooxygenase-2 (COX-2) and down-regulation of 15-prostaglandin dehydrogenase (15-PGDH) have been linked to the switch for a pro-proliferative and pro-angiogenic tumor microenvironment. Previous studies have characterized the effects of increased prostaglandin E2 in tumor progression but the role of many other endogenous lipid mediators has yet to be determined. COX-2 can metabolize arachidonic acid (AA) to hydroxyeicosatetraenoic acids (HETEs), which in turn can be further oxidized by dehydrogenases into oxo-eicosatetraenoic acids (oxo-ETEs). We have previously reported that 11-oxo-ETE and 15-oxo-ETE are major COX-2/15-PGDH derived metabolites formed from human cells expressing COX-2 treated with AA. Although no known G-protein coupled receptor has been reported for either of these metabolites, both 11-oxo- and 15-oxo-ETE have been described from human clinical samples. This study provides a description of the uptake and distribution of 11-oxo-ETE and 15-oxo-ETE as well as their anti-proliferative activity in both human umbilical vein endothelial cells (HUVECs) and LoVo human colon adenocarcinoma cells. Quantification of the intracellular and extracellular levels of 11-oxo- and 15-oxo-ETE was conducted using liquid chromatography electron capture atmospheric pressure chemical ionization mass spectrometry with [13C]-labeled 15-oxo-ETE as an internal standard. The intracellular concentrations of 11-oxo- and 15-oxo-ETE reached peak amounts within 1h followed by a rapid decline. Earlier studies have indicated that glutathione (GSH)-S-transferase further metabolized 11-oxo-ETE to form the 11-oxo-ETE-GSH (OEG)-adduct. In additional experiments, pretreatment with ethacrynic acid (EA), a known inhibitor of GSTs, increased recovery from the cellular extract. In addition, the methyl-ester of both 11-oxo- and 15-oxo-ETE was used for the targeted intracellular delivery of these COX-2/15-PGDH derived eicosanoids. Finally, we have shown that both 11-oxo- and 15-oxo-ETE as well as their methyl ester derivatives significantly inhibit cell proliferation as determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell proliferation assay and Bromodeoxyuridine (BrdU) incorporation in multiple human cell lines. The present study has established the cellular uptake of the short-lived COX-2/15-PGDH derived eicosanoids 11-oxo- and 15-oxo-ETE and potent inhibition of new DNA synthesis and proliferation. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-10. doi:1538-7445.AM2012-LB-10
Potentiated Angiogenic Effect of Scatter Factor/Hepatocyte Growth Factor via Induction of Vascular Endothelial Growth Factor
Authors: Eric Van Belle, Bernhard Witzenbichler, Donghui Chen, Marcy Silver, Ling Chang, Ralph Schwall, Jeffrey M. Isner
Journal: Circulation (1998)

Background —Scatter factor/hepatocyte growth factor (SF/HGF) is a pleiotropic growth factor that stimulates proliferation and migration of endothelial cells (ECs) via the c- Met receptor, present on ECs as well as other cell types, including smooth muscle cells (SMCs). We studied the effects of recombinant human (rh) SF/HGF in vitro and in vivo in a rabbit model of hindlimb ischemia. We further compared these effects with those of recombinant human vascular endothelial growth factor (rhVEGF 165 ), an EC–specific mitogen. Methods and Results —In vitro, rhSF/HGF and rhVEGF 165 exhibited similar effects on proliferation and migration of ECs. When both cytokines were administered together, the result was an additive effect on EC proliferation and a synergistic effect on EC migration. Application of rhSF/HGF to cultures of human SMCs resulted in the induction of VEGF mRNA and protein. In vivo, administration of rhSF/HGF (500 μg×3) was associated with significant improvements in collateral formation ( P &lt;.001) and regional blood flow ( P &lt;.0005) and with a significant reduction in muscle atrophy ( P &lt;.0001). These effects were significantly more pronounced than those of rhVEGF 165 administered according to the same protocol ( P &lt;.05). Neither remote angiogenesis nor other pathological sequelae were observed with either rhSF/HGF or rhVEGF 165 . Conclusions —The pleiotropic effects of certain growth factors may potentiate angiogenesis via a combination of direct effects on EC proliferation and migration and indirect effects that result in the generation of other potent EC mitogens from non-EC populations. The synergistic effects demonstrated when SF/HGF and VEGF are administered together in vitro may be reproduced in vivo by SF/HGF-induced upregulation of VEGF in vascular SMCs.
Eyad Rajab el-Sarraj
Authors: S. Arie
Journal: BMJ (2014)

Palestinian psychiatrist, rights campaigner, and self styled diplomat



MAHMUD HAMS/AFP/Getty Images

In late 2008, when Israel began a bombing campaign of Gaza in response to years of rocket attacks by Palestinian militants, Eyad el-Sarraj could have stayed with his wife and their 3 year old son in the safety and comfort of their Geneva home. He was 65 and had been diagnosed as having leukaemia two years earlier, but he chose to be in the Gaza Strip during three of the most violent weeks in its recent history.

El-Sarraj, a Palestinian who trained in Egypt and Britain, spent much of his career living and working in the poverty and turmoil of Gaza—despite having British citizenship, which could have allowed him a more comfortable life. He began to practise in Gaza in the 1980s and is thought to have been the first psychiatrist to do so. In 1990 he established the Gaza Community Mental Health Programme—a non-governmental organisation providing clinical support to a society traumatised by the first Intifada, but with no culture of talking …
Review: Acamprosate increases abstinence in patients with alcohol dependence
Authors: Jennifer McNeely, Scott Sherman
Journal: Annals of Internal Medicine (2011)

ACP Journal Club18 January 2011Review: Acamprosate increases abstinence in patients with alcohol dependenceJennifer McNeely, MD, MS, Scott Sherman, MD, MPHJennifer McNeely, MD, MSNew York University School of Medicine, New York, New York, USA (J.M., S.S.)Search for more papers by this author, Scott Sherman, MD, MPHNew York University School of Medicine, New York, New York, USA (J.M., S.S.)Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-154-2-201101180-02010 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Source CitationRösner S, Hackl-Herrwerth A, Leucht S, et al. Acamprosate for alcohol dependence. Cochrane Database Syst Rev. 2010;(9):CD004332. https://pubmed.ncbi.nlm.nih.gov/20824837Clinical Impact RatingsGIM/FP/GP: Mental Health: References1 Mokdad AH, Marks JS, Stroup DF, Gerberding JL. Actual causes of death in the United States, 2000. JAMA. 2004;291:1238-45. [PMID: 15010446] Google Scholar2 Substance Abuse and Mental Health Services Administration, Office of Applied Studies. Results from the 2007 National Survey on Drug Use and Health: national findings. 2008. www.oas.samhsa.gov/NSDUH/2k7NSDUH/2k7results.cfm#Ch7 (accessed 25 Nov 2010). Google Scholar3 Anton RF, O’Malley SS, Ciraulo DA, et al. Combined pharmacotherapies and behavioral interventions for alcohol dependence: the COMBINE study: a randomized controlled trial. JAMA 2006;295:2003-17. [PMID: 16670409] Google Scholar4 Mason BJ, Goodman AM, Chabac S, Lehert P. Effect of oral acamprosate on abstinence in patients with alcohol dependence in a double-blind, placebo-controlled trial: the role of patient motivation. J Psychiatr Res. 2006;40:383-93. [PMID: 16546214] Google Scholar Author, Article, and Disclosure InformationAuthors: Jennifer McNeely, MD, MS; Scott Sherman, MD, MPHAffiliations: New York University School of Medicine, New York, New York, USA (J.M., S.S.)This article was published at Annals.org on 4 January 2011. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byThe cost-effectiveness of tailored, postal feedback on general practitioners’ prescribing of pharmacotherapies for alcohol dependenceBaclofen has opposite effects on escalation of cocaine self-administration: Increased intake in rats selectively bred for high (HiS) saccharin intake and decreased intake in those selected for low (LoS) saccharin intake 18 January 2011Volume 154, Issue 2Page: JC1-10KeywordsAlcoholismDiarrheaDrug dependenceDrugsNumber needed to harmNumber needed to treatOutpatientsPatientsPsychiatry and mental healthRelative risk ePublished: 18 January 2011 Issue Published: 18 January 2011 Copyright & PermissionsCopyright © 2011 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
The Man Who Worked Too Hard
Authors: Andrew D. Coates
Journal: Annals of Internal Medicine (2013)

On Being a DoctorSeptember 17, 2013The Man Who Worked Too HardAndrew D. Coates, MDAndrew D. Coates, MDFrom Albany Medical College, Albany Medical College, Albany, New York.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-159-6-201309170-00014 Audio Reading - “The Man Who Worked Too Hard” Audio. Michael A. LaCombe, MD, Annals Associate Editor, reads "The Man Who Worked Too Hard," by A.D. Coates. Your browser does not support the audio element. Audio player progress bar Step backward in current audio track Play current audio trackPause current audio track Step forward in current audio track Mute current audio trackUnmute current audio track 00:00/ SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Influenza rocked our community hospital for weeks this winter just as the first snowstorms came. Our hospital medicine service was swamped with patients. I was working the night shift.One emergency physician dramatically recounted how a queue of ambulances had clogged the parking lot. Once all of the emergency department beds, hallway spaces, and waiting room seats were taken, patients waited outside in ambulances. With the usual triage system in gridlock, he had climbed into ambulances one by one to help triage or stabilize patients.Such are the undignified consequences of a system that encourages hospitals to run themselves like ... Author, Article, and Disclosure InformationAffiliations: From Albany Medical College, Albany Medical College, Albany, New York.Corresponding Author: Andrew D. Coates, MD, Department of Medicine, Albany Medical College, 47 New Scotland Avenue, MC 57, Albany, NY 12208; e-mail, [email protected]. PreviousarticleNextarticle Advertisement Audio Reading - “The Man Who Worked Too Hard” Audio. Michael A. LaCombe, MD, Annals Associate Editor, reads "The Man Who Worked Too Hard," by A.D. Coates. Your browser does not support the audio element. Audio player progress bar Step backward in current audio track Play current audio trackPause current audio track Step forward in current audio track Mute current audio trackUnmute current audio track 00:00/ FiguresReferencesRelatedDetails Metrics September 17, 2013Volume 159, Issue 6Page: 433-434KeywordsAbscessesAllied health care professionalsAmbulancesBeesCardiovascular therapyComputed axial tomographyEatingEmotionsFoodHealth insuranceHospital medicineHospitalizationsMyocardial infarctionPreventive medicineResearch laboratoriesRunningStrokeTriageUrticariaVital signs ePublished: 17 September 2013 Issue Published: September 17, 2013 Copyright & PermissionsCopyright © 2013 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Japan moves towards legal ban on human cloning
Authors: J. Lamar
Journal: BMJ (1999)

Japan moved closer to imposing a legal ban on human cloning when an advisory body to its prime minister, Keizo Obuchi, ruled last week that current guidelines were inadequate.

The panel, a subcommittee of the Science and Technology Council, argued that cloning has no positive utility, runs counter to respect for human life, and poses …
Child vaccination campaigns are suspended in Syria after 15 infants die
Authors: S. Arie
Journal: BMJ (2014)

Vaccination campaigns in Syria face serious disruption after at least 15 infants aged under 2 died from receiving a heavy dose of anaesthetic by mistake, when they were supposed to receive a measles vaccine.

The infants in the northern, opposition controlled provinces of Idlib and Deir el Zour showed signs of shock and then suffocated shortly after receiving atracurium, a neuromuscular blocking agent used to temporarily paralyse the respiratory muscles …
British Society of Gastroenterology guidelines on the diagnosis and management of coeliac disease
Authors: Peter Hill, Andrew Austin, Julia Forsyth, Geoffrey Holmes
Journal: Gut (2014)

The British Society of Gastroenterology (BSG) guidelines1 on diagnosis and management of coeliac disease (CD) contain much useful information, but in our opinion are badly misleading on the important topic of diagnosis and the relative merits of small intestinal biopsy and serology tests. The authors recommend that a duodenal biopsy is essential for diagnosis in all patients and cannot be replaced by serology alone even in a subset of patients.

Other studies have now confirmed earlier work showing that using an appropriate cut-off for IgA-class anti-tissue transglutaminase antibody (IgA-tTG), the diagnosis can be made with a positive predictive value …
THE FUNDAMENTAL PROPERTIES OF THE FIBROBLAST AND THE MACROPHAGE
Authors: Alexis Carrel, Albert H. Ebeling
Journal: The Journal of Experimental Medicine (1928)

1. A pure strain of fibroblasts has been isolated from the Jensen rat sarcoma. The cells give rise to tumors on transplantation into animals and during several months of life in vitro have maintained their malignancy unchanged. 2. The malignant cells are generally coarser and more refringent than normal cells. They possess the cytological characteristics of fibroblasts without showing any morphological abnormality. They can be considered as healthy cells. The texture of their colonies is looser than that of normal fibroblasts and Sarcoma No. 10 fibroblasts. Their residual activity does not differ markedly from the normal. They proliferate unlimitedly in a nutrient medium. 3. They liquefy the fibrin of rat plasma and turn phenol red golden yellow. They do not liquefy the fibrin of chicken plasma. 4. They multiply in chick embryo juice, calf liver digest, and also in rat serum. Their growth activity is increased by the presence of bone marrow. 5. The unlimited growth of Jensen sarcoma within the body may possibly be attributed to the ability of the fibroblasts to maintain themselves upon the substances present in rat serum. This property itself probably depends upon the increased enzyme activity of the cells.
Dramatic reduction of clindamycin serum concentration in staphylococcal osteoarticular infection patients treated with the oral clindamycin-rifampicin combination
Authors: Aurélie Bernard, Gwénolé Kermarrec, Perrine Parize, Thibaut Caruba, Anaïs Bouvet, Jean-Luc Mainardi, Brigitte Sabatier, Christophe Nich
Journal: Journal of Infection (2015)
Patterns of Breast Feeding
Authors: Eva J. Salber, Pauline G. Stitt, Joan G. Babbott
Journal: New England Journal of Medicine (1958)

THE frequency of breast feeding and the factors that affect it were investigated in 114 women who attended the Family Health Clinic in Boston between 1950 and 1956. The material will be presented in two papers — the present article and one to be published later on entitled "Duration of Breast Feeding."The Family Health ClinicThe organization of the Family Health Clinic has been discussed in several publications,1 2 3 4 and need not be dealt with in great detail here. The scheme encompassed both a service and a research project and explored the possibility of a team approach to family health . . .
Constraining the brain: The role of developmental psychology in developmental cognitive neuroscience
Authors: David Estes, Karen Bartsch
Journal: Behavioral and Brain Sciences (1997)

Developmental psychology should play an essential constraining role in developmental cognitive neuroscience. Theories of neural development must account explicitly for the early emergence of knowledge and abilities in infants and young children documented in developmental research. Especially in need of explanation at the neural level is the early emergence of meta-representation.
Retinoic Acid Embryopathy: Timing of Exposure and Effects on Fetal Development
Authors: Joseph H. Hersh
Journal: JAMA (1985)

<h3>To the Editor.—</h3> Inadvertent use of isotretinoin (13-<i>cis</i>-retinoic acid), an oral synthetic vitamin A analogue, in the treatment of cystic acne in utero, has resulted in fetal wastage and congenital malformations primarily characterized by ear and eye anomalies, cardiac malformations including conotruncal lesions, and central ner
Immunoreactive Forms of Circulating Parathyroid Hormone in Primary and Ectopic Hyperparathyroidism
Authors: Ralph C. Benson, B. Lawrence Riggs, Barbara M. Pickard, Claude D. Arnaud
Journal: Journal of Clinical Investigation (1974)

The immunoreactive forms of parathyroid hormone (iPTH) in the plasma of six patients with primary, adenomatous hyperparathyroidism and six patients with ectopic hyperparathyroidism due to non-parathyroid cancer were compared by using gel filtration on columns of Bio-Gel P-150 and radioimmunoassay of iPTH in eluted fractions after concentration. We found much less (p<0.001) small (mol wt<9,500) COOH-terminal fragments of iPTH in plasma samples from ectopic hyperparathyroid patients (0.52+/-0.13 ng eq/ml) than in samples from primary hyperparathyroid patients (3.70+/-1.15 ng eq/ml). The quantity of iPTH eluting with or before native bovine PTH [1-84] was the same in both syndromes (ectopic hyperparathyroidism, 0.82+/-0.22 ng eq/ml; primary hyperparathyroidism, 0.73+/-0.09 ng eq/ml), and these values correlated positively with plasma calcium concentration (ectopic hyperparathyroidism, r=0.908; primary hyperparathyroidism, r=0.919). In both syndromes, plasma samples had an iPTH component that eluted well before PTH [1-84] (mol wt 9,500), but this component was present in much larger quantities in three patients with ectopic hyperparathyroidism. We conclude that (a) the decreased quantity of biologically inactive COOH-terminal fragments of iPTH circulating in ectopic hyperparathyroidism accounts for the previously reported relatively lower total serum iPTH values in this syndrome as compared with primary hyperparathyroidism (Riggs et al. 1971. J. Clin. Invest. 50: 2079); (b) there appears to be sufficient iPTH with presumed biologic activity to account for the hypercalcemia in both syndromes; (c) a large PTH component, not previously recognized in plasma, is present in both ectopic and primary hyperparathyroidism and may exist as the predominant immunoreactive form of the hormone in some patients with ectopic hyperparathyroidism.
Who Will Give Primary Care?
Authors: Lynn P. Carmichael
Journal: JAMA (1971)

The Association of American Medical Colleges met in Los Angeles Oct 29 to Nov 2, 1970. A large portion of the meeting was devoted to the health manpower crisis in primary health care and the vitalized family practice movement. The papers presented often mentioned the physician's assistant and the role of the physician. Throughout the discussions it was evident that many thought primary care was less demanding and should be assigned to those of lesser training and status.  There is a widespread assumption that creating the physician was what the Lord did on the eighth day. Not so! Society begat physicians to meet their needs and demands in a particular area. As society rewards those who meet its wishes with prestige, financial success and other advantages, so has society rewarded the medical profession. If physicians abandon primary care for the categorical specialties, will these rewards continue? It seems that, if