tbuckley/cabot-search huggingface dataset:
Abstract 2594: 15-Deoxy-Δ12,14-prostaglandin J2 induces apoptosis through covalent interaction with STAT3: structure-activity relationship and underlying molecular mechanisms.
Authors: Su-Jung Kim, Young-Il Hahn, Bu Young Choi, Young-Ger Suh, Hye-Kyung Na, Young-Joon Surh
Journal: Cancer Research (2013)

Abstract Our previous studies demonstrated that 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2), a natural ligand for peroxisome proliferator-activated receptor γ, inhibited cell proliferation and induces apoptosis in several cancerous and transformed cell lines. 15d-PGJ2 contains α,β-unsaturated carbonyl groups that can form reversible adducts with thiol nucleophiles. In the present work, we synthesized a series of 15d-PGJ2 analogues by saturating an electrophilic α,β-unsaturated carbonyl moiety in the cyclopentenone ring. Among these, 14,15-dihydro-PGJ2 with a more electrophilic carbon exhibited a potent growth inhibitory activity and induced apoptosis to a greater extent than did 15d-PGJ2. 14,15-Dihydro-PGJ2 as well as 15d-PGJ2 inhibited activation of STAT3, which appeared to be associated with their direct binding to this transcription factor. In contrast, both 9,10-dihydro-15d-PGJ2 and 12,13-14,15-tetrahydro-PGJ2 failed to induce apoptosis and STAT3 activation due to lack of the electrophilic α,β-unsaturated carbonyl moiety. Molecular docking studies suggested that the electrophilic carbon atom of 14,15-dihydro-PGJ2 is placed well in the vicinity of Cys259 of STAT3 to form a covalent Michael adduct. Site-directed mutation of Cys259 of STAT3 abolished its interaction with 15d-PGJ2. 14,15-dihydro-PGJ2 can stably interacted with STAT3, this synthetic cyclopentenone analogue can hence be utilized as a lead compound to develop STAT3 specific inhibitors with anti-tumorigenic activity. Citation Format: Su-Jung Kim, Young-Il Hahn, Bu Young Choi, Young-Ger Suh, Hye-Kyung Na, Young-Joon Surh. 15-Deoxy-Δ12,14-prostaglandin J2 induces apoptosis through covalent interaction with STAT3: structure-activity relationship and underlying molecular mechanisms. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2594. doi:10.1158/1538-7445.AM2013-2594
Terrorism and Public Health: A Balanced Approach to Strengthening Systems and Protecting People
Authors: Amesh Ashok Adalja
Journal: JAMA (2012)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Pyogenic lymphoma of the skin: a peculiar variant of primary cutaneous neutrophil-rich CD30+ anaplastic large-cell lymphoma. Clinicopathological study of four cases and review of the literature
Authors: G Burg, W Kempf, D.V Kazakov, R Dummer, P.J Frosch, S Lange-Ionescu, T Nishikawa, M.E Kadin
Journal: British Journal of Dermatology (2003)

Journal Article Pyogenic lymphoma of the skin: a peculiar variant of primary cutaneous neutrophil‐rich CD30+ anaplastic large‐cell lymphoma. Clinicopathological study of four cases and review of the literature Get access G Burg, G Burg Department of Dermatology, University Hospital Zurich, CH‐8091 Zurich, Switzerland Günter Burg. E‐mail: burg@derm.unizh.ch Search for other works by this author on: Oxford Academic Google Scholar W Kempf, W Kempf Department of Dermatology, University Hospital Zurich, CH‐8091 Zurich, Switzerland Search for other works by this author on: Oxford Academic Google Scholar D.V Kazakov, D.V Kazakov Department of Dermatology, University Hospital Zurich, CH‐8091 Zurich, Switzerland Search for other works by this author on: Oxford Academic Google Scholar R Dummer, R Dummer Department of Dermatology, University Hospital Zurich, CH‐8091 Zurich, Switzerland Search for other works by this author on: Oxford Academic Google Scholar P.J Frosch, P.J Frosch Department of Dermatology, Clinics of Dortmund, University of Witten/Herdecke, Germany Search for other works by this author on: Oxford Academic Google Scholar S Lange‐Ionescu, S Lange‐Ionescu Department of Dermatology, Clinics of Dortmund, University of Witten/Herdecke, Germany Search for other works by this author on: Oxford Academic Google Scholar T Nishikawa, T Nishikawa Department of Dermatology, Keio University School of Medicine, Tokyo, Japan Search for other works by this author on: Oxford Academic Google Scholar M.E Kadin M.E Kadin Department of Pathology, Division of Hematopathology, Beth Israel Deaconess Medical Center, Boston, MA, U.S.A Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 148, Issue 3, 1 March 2003, Pages 580–586, https://doi.org/10.1046/j.1365-2133.2003.05248.x Published: 01 March 2003 Article history Accepted: 09 October 2002 Published: 01 March 2003
We cannot afford an AIDS epidemic.
Authors: M Bailey
Journal: BMJ (1991)

Pharmacological activators of peroxisome proliferator-activated receptor-γ (PPARγ) inhibit growth of non-small-cell lung cancer (NSCLC) cell lines in vitro and in xenograft models. Because these agents engage off-target pathways, we have assessed the effects of PPARγ by overexpressing the protein in NSCLC cells. We reported previously that increased PPARγ inhibits transformed growth and invasiveness and promotes epithelial differentiation in a panel of NSCLC expressing oncogenic K-Ras. These cells express high levels of cyclooxygenase-2 (COX-2) and produce high levels of prostaglandin E<sub>2</sub> (PGE<sub>2</sub>). The goal of these studies was to identify the molecular mechanisms whereby PPARγ inhibits tumorigenesis. Increased PPARγ inhibited expression of COX-2 protein and promoter activity, resulting in decreased PGE<sub>2</sub> production. Suppression of COX-2 was mediated through increased activity of the tumor suppressor phosphatase and tensin homolog, leading to decreased levels of phospho-Akt and inhibition of nuclear factor-κB activity. Pharmacological inhibition of PGE<sub>2</sub> production mimicked the effects of PPARγ on epithelial differentiation in three-dimensional culture, and exogenous PGE<sub>2</sub> reversed the effects of increased PPARγ activity. Transgenic mice overexpressing PPARγ under the control of the surfactant protein C promoter had reduced expression of COX-2 in type II cells and were protected against developing lung tumors in a chemical carcinogenesis model. These data indicate that high levels of PGE<sub>2</sub> as a result of elevated COX-2 expression are critical for promoting lung tumorigenesis and that the antitumorigenic effects of PPARγ are mediated in part through blocking this pathway.
A Randomized Clinical Trial Comparing Behavior Modification and Individual Counseling in the Nutritional Therapy of Non-insulin-dependent Diabetes Mellitus: Comparison of the Effect on Blood Sugar, Body Weight, and Serum Lipids
Authors: Simon W Rabkin, Evelyn Boyko, Allan Wilson, Dan A Streja
Journal: Diabetes Care (1983)

To determine whether a group behavior modification approach might be preferable to individual counseling in the nutritional therapy of non-insulin-dependent diabetes mellitus, 40 adults younger than 65 yr of age with diabetes mellitus who were not receiving insulin were randomized to either a program of individualized dietary review and recommendations or a program of group meetings aimed at controlling the signals leading to overeating and noncompliance with a diabetic dietary regimen. Statistically significant (P &amp;lt; 0.05) decreases in body weight, sum skin-fold thickness, fasting serum glucose, and serum triglycerides but not LDL-C or HDL-C were observed. The individual counseling group had a greater amount of weight loss than the behavior modification group. There were no significant (P &amp;lt; 0.05) differences between the two groups with respect to the biochemical outcome variables. Patient characteristics assessed at entry—namely anxiety, internal versus external locus of control and perceived disease severity, and compliance with advice—were significantly associated with weight loss in the behavior modification group while only the latter index was of value in the individual counseling group. Thus, our use of these programs does not identify a clear advantage of either approach in the nutritional therapy of non-insulin-dependent diabetic patients.
Radioimmunoassay of human parathyroid hormone in serum
Authors: Claude D. Arnaud, Hang S. Tsao, Travis Littledike
Journal: Journal of Clinical Investigation (1971)

A new radioimmunoassay for human parathyroid hormone (PTH) in serum, which can measure the hormone present in 94% of the normal sera tested, is described. It is based on the ability of human PTH to compete with (131)I-labeled bovine PTH for binding to an antiserum directed against porcine PTH. This antiserum distinguishes between human PTH extracted from parathyroid adenomata and that present in hyperparathyroid sera. Evidence is given to suggest that this is due to immunochemical changes in the hormone extracted from adenomata and not to immunochemical heterogeneity of the hormone present in serum. Physiologic data supporting the validity and specificity of the assay are presented. Induced episodes of hypercalcemia and hypocalcemia resulted in appropriate responses in serum immunoreactive PTH (IPTH) in normal subjects and in patients with Paget's disease of bone. In normals, there was a progressive increase in serum IPTH in the late afternoon and evening, suggesting a diurnal secretory rhythm. A negative correlation was found between the serum calcium and serum IPTH over the normal range of serum calcium values; a positive correlation was found between these variables in patients with primary hyperparathyroidism. There was apparent overlap between serum IPTH values in normal subjects and patients with primary hyperparathyroidism, but formal discriminate analysis of values for serum calcium and IPTH demonstrated separation of these two groups, without overlap.
A novel peptide therapeutic targeting PD1 immune checkpoint with equipotent antagonism of both ligands and a potential for better management of immune-related adverse events
Authors: Pottayil Sasikumar, Rajeev Shrimali, Sreenivas Adurthi, Raghuveer Ramachandra, Leena Satyam, Amit Dhudashiya, Dodheri Samiulla, K B Sunilkumar, Murali Ramachandra
Journal: Journal for ImmunoTherapy of Cancer (2013)

Meeting abstracts Recent advances in achieving highly durable clinical responses via inhibition of immune checkpoint proteins including CTLA-4 and PD1 have revolutionized the outlook for cancer therapy. However, along with impressive clinical activity (response rate of ~25% with either anti-CTLA-4
Use of specific fluorescent antibodies for the identification of hemoglobin C in erythrocytes
Authors: TH. Papayannopoulou, V. Ahern, G. Stamatoyannopoulos, G. Lim, P. E. Nute, T. C. McGuire
Journal: American Journal of Hematology (1977)

Antibodies against hemoglobulin C (alpha2beta2 6Glu leads to Lys) were produced by immunizing horses and were purified by affinity chromatography. As expected from the bivalency of both the antibody and the antigen, the purified antibodies failed to produce immunoprecipitates upon reaction with the corresponding antigens. Identification of hemoglobin C in individual erythrocytes was achieved by reacting the fluorescein isothiocyanate-conjugated antibodies with the hemoglobin antigen in fixed smears of peripheral blood. Red cells from persons having a hemoglobin C gene were labeled strongly upon reaction with anti-Hb C-FITC; there was no labeling of red cells containing normal hemoglobins or Hb S, suggesting that the anti-Hb C antibodies recognize only the amino-terminal segment of the beta chains that contain lysine in position beta6.
Abstract 1092: Insulin-like growth factors modulate chemosensitivity to cisplatin and 5-fluorouracil in human esophageal cell lines
Authors: Ronghua Zhao, Alan G. Casson
Journal: Cancer Research (2011)

Abstract Resistance to chemotherapy remains a major obstacle to survival of patients with esophageal adenocarcinoma (EADC). The aim of this study was to evaluate whether insulin-like growth factors (IGFs), recently implicated in the molecular pathogenesis of EADC, modulate chemosensitivity to Cisplatin (CDDP) and 5-Fluoruracil (5-Fu), two agents widely used in current clinical practice. Expression of IGF1, its receptor (IGF1R), and IGF2 (mRNA and protein) were studied using quantitative (real-time) PCR and Western blot in human esophageal cell lines: Het1A (derived from immortalized normal esophageal epithelium), OE33 and JHEsoAd1 (each derived from a primary EADC). For each cell line, the effect of various concentrations of IGFs, CDDP and 5-Fu, alone and in combination, on cell proliferation and clonogenicity were evaluated by standard MTT and colony formation assays, respectively. Changes in expression of 84 critical genes downstream from IGF1R were studied by PCR-array. Relative to Het1A, IGF1 and IGF2 mRNA were significantly (P&amp;lt;0.05) underexpressed by OE33; by contrast, JHEsoAd1 underexpressed IGF1 but overexpressed IGF2. Administration of either IGF1 (250ng/ml) or IGF2 (500ng/ml) to cell cultures resulted in increased cellular proliferation of JHEsoAD1 cells (after IGF1: 1.24+/-0.05 vs. 1.00+/-0.00 untreated, P&amp;lt;0.01; after IGF2: 1.15+/-0.06 vs. 1.00+/-0.06 untreated, P&amp;lt;0.05). As expected, cell proliferation and clonogenicity of all cell lines were inhibited by CDDP and/or 5-FU (at all dosages ranging from 1-4ug/ml). However, these cytotoxic effects were overcome by the administration of either IGF1 or IGF2 with the exception of: 1) JHEsoAd1 cells treated with CDDP, where IGF2 administration further reduced cellular proliferation (0.35+/-0.06 vs. 0.53+/-0.03 for cells treated with CDDP alone; P&amp;lt;0.05), and 2) JHEsoAd1 cells treated with 5-Fu, where IGF1 administration significantly reduced clonogenicity (0.07+/-0.07 vs. 0.49+/-0.06 for cells treated with 5-Fu alone; P&amp;lt;0.05). PCR-array analysis revealed up-regulation (Het1A and JHEsoAd1) and down-regulation (OE33 and JHEsoAd1) of selected PI3K/AKT pathway genes following IGF1 and IGF2 therapy. These pre-clinical studies confirm a central role for the IGF axis in esophageal tumor biology, with potential for IGFs to enhance the cytotoxic efficacy of standard chemotherapeutic agents in esophageal cell lines. The identification of novel molecular regulatory pathways downstream from IGF1R modulated by IGF therapy may well inform future cytotoxic and targeting strategies. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1092. doi:10.1158/1538-7445.AM2011-1092
William Dameshek and Frederick Gunz's Leukemia
Authors: D. P. Shreiner
Journal: JAMA (1975)

<b>Modern Trends in Human Leukemia: Biological, Biochemical, and Virological Aspects,</b>edited by Rolf Neth et al (workshop, June 1973), 366 pp, with illus, $29.50, New York, Grune &amp; Stratton, 1974. The third edition of<i>Leukemia</i>, originally edited by Dameshek and Gunz, has doubled in size since the first edition. It is quite readable and up-to-date, at least as much as any textbook in this rapidly changing field can be. An attempt has been made to make this volume even more current by adding a final chapter on recent advances in this field, but the title is somewhat misleading in that paraproteinemias and lymphomas are also covered. There are relatively few errors for a book of this size (over 800 pages), and the small number of contributors (six) provides a sense of continuity. However, it suffers from needless repetition that may tend to make each chapter more complete but unnecessarily lengthens
An Appeal: To the Scientists of the American Medical Association
Authors: G. T. Sushko
Journal: JAMA (1982)

To the Editor.—  Respected colleagues, I work as a physician in the Soviet Union, and I appeal to you with regard to the extremely complex international situation that is balancing on the edge of a war destructive to all living things on our planet through the use of thermonuclear, chemical, biologic, meteorological, and other varieties of qualitatively new weapons of mass annihilation. Such war, never yet seen on earth, can break out at any moment, even completely by accident, which is aided by the already present huge arsenals of weapons of mass annihilation (equivalent to 20 tons of conventional explosives for each of the earth's inhabitants!) and the means of their lightninglike delivery to their targets.  As witnessed by our numerous scientific investigations during the course of the last 15 to 20 years, the courses of many diseases in people have changed. A series of inflammatory diseases run their course
Polyethylene glycol modification of interleukin-6 enhances its thrombopoietic activity
Authors: Yasuo Tsutsumi, Tetsunari Kihira, Shin-ichi Tsunoda, Naoki Okada, Yoshihisa Kaneda, Yoshiyuki Ohsugi, Masaharu Miyake, Shinsaku Nakagawa, Tadanori Mayumi
Journal: Journal of Controlled Release (1995)
Total Serum IgE Levels in Normals and Patients with Chronic Non–Specific Lung Diseases
Authors: R. Pauwels, M. Van Der Straeten
Journal: Allergy (1978)

Total serum IgE was measured, using a sensitive sandwich method, in a healthy control population, in healthy persons with a familial history of chronic non‐specific lung disease and in patients with chronic non‐specific lung disease (CNSLD). In the control population a median total serum IgE of 39 1.U./ml was found. Total serum IgE levels were significantly lower in women that in men. Healthy persons with a familial history of CNSLD had significantly increased total IgE levels compared with the control population. The IgE level was even higher in the group of patients with CNSLD The presence of specific IgE antibodies and/or an early onset of the symptoms of CNSLD was accompanied by higher total serum IgE levels compared with the IgE values in the other CNSLD patients. But the total IgE levels were increased in all subgroups of CNSLD patients. A significant decrease of the IgE level with age was observed in CNSLD patients.
Cardiac progenitor cell sheet regenerates myocardium and renews hope for translation
Authors: P. W. M. Fedak
Journal: Cardiovascular Research (2010)

This editorial refers to ‘Transplantation of cardiac progenitor cell sheet onto infarcted heart promotes cardiogenesis and improves function’ by L. Zakharova et al ., pp. 40–49, this issue. 

The goal of myocardial regeneration strategies is to transform dead scar tissue into viable functional muscle. If achieved, we could eradicate congestive heart failure. Early work using cell suspensions with delivery by injection showed promise, but substantial regeneration has remained elusive for our patients, mostly due to poor viability and donor cell retention after transplantation. The enthusiasm for this strategy is fading.

The innovative work of Zakharova et al .1 could restore hope and help guide us in the right direction. The authors assembled cardiac progenitor cells into a tissue-like sheet of cells and then delivered the construct onto the epicardial surface of an acute rat infarction. This unique approach enhanced stem cell engraftment and survival, stimulated a substantial cardiogenesis, and preserved left ventricular function to an extent that could prevent the transition to overt heart failure. Importantly, the donor cells did more than survive in the host myocardium; after engraftment, they showed an encouraging combination of controlled proliferation, purposeful migration, and targeted differentiation. The authors then extended, confirmed, and reproduced these results using human progenitor cells obtained from surgical biopsy. The potential for translation to our patients using this strategy is exciting.

Progress in using cell …
Heparin in Subacute Bacterial Endocarditis
Authors: G. C. Dockeray, E. Kawerau
Journal: BMJ (1940)

MEBRITAISH 703The exacting routine of repeated applications of gentian violet, silver nitrate, and tannic acid solutions to produce a dry smooth coagulum is well justified by results.We are indebted to Major H. G. Hobson, R.A.M.C., for permission to record these cases, for much help in carrying out their treatment, and for advice and many hints in recording them.
Human auto-antiidiotypes regulating T cell-mediated reactivity to tetanus toxoid.
Authors: A Saxon, E Barnett
Journal: Journal of Clinical Investigation (1984)

While investigating the effect on B cells of repetitive in vivo immunization with tetanus toxoid (TT), we observed the subsequent development of specific anergy for T cell delayed hypersensitivity (DTH) to TT. This appeared approximately 35 d after a series of five booster immunizations. Concurrently, in vitro T cell blastogenic responses were preserved. Serum obtained when the skin tests were nonreactive demonstrated a profound inhibitory activity on T cell reactivity. This activity was shown to be anti-antibody activity that was both anti-F(ab)'2 and, specifically, anti-TT F(ab)'2. It blocked binding of TT to a pool of allogeneic antibodies and also inhibited allogeneic antigen-specific T cell blastogenesis. Thus, we could identify activity in the serum of hyperimmunized individuals that appeared auto-anti-idiotypic (anti-id) and represented a single or family of major crossreacting idiotypes (id) for TT. The expression of the auto-anti-id correlated with the loss of T cell reactivity in vivo and in vitro. Subsequent examinations revealed persistent, specific cutaneous anergy beyond six months, which was then associated with a failure of T cells to react with antigen in vitro. Mixing experiments with cells from these later times and cryopreserved autologous cells obtained prior to hyperimmunization revealed there had been the development of antigen-specific T suppressor cells. Thus, in vivo DTH tolerance following hyperimmunization was associated with an inhibitory serum activity that appeared to be anti-id. Persistence of tolerance (greater than 6 mo) occurred with the development of T suppressor cells.