tbuckley/cabot-search huggingface dataset:
Novel and effective gene transfer technique for study of vascular renin angiotensin system.
Authors: R Morishita, G H Gibbons, Y Kaneda, T Ogihara, V J Dzau
Journal: Journal of Clinical Investigation (1993)

Vascular renin angiotensin system (RAS) has been reported to exist in vascular wall. However, there is no direct evidence whether the vascular RAS per se can modulate growth of vascular smooth muscle cells (VSMC), because there is no suitable method to investigate the effect of endogenously produced vasoactive substances on growth of these cells. In this study, we transferred angiotensin-converting enzyme (ACE) and/or renin cDNAs into cultured VSMC using the efficient Sendai virus (hemagglutinating virus of Japan) liposome-mediated gene transfer method, to examine their relative roles in VSMC growth in vitro. Within 35 min or 6 h, the transfection of ACE cDNA into VSMC by hemagglutinating virus of Japan method resulted in a twofold higher ACE activity than control vector, whereas a cationic liposome (Lipofectin)-mediated method failed to show any effect. This in vitro system provided us with the opportunity to investigate the influence of endogenous vascular RAS on VSMC growth. Transfection of ACE or renin cDNA resulted in increased DNA and RNA synthesis, which was inhibited with the specific angiotensin II receptor antagonist (DuP 753: 10(-6) M). Angiotensin I added to ACE-transfected VSMC increased RNA synthesis in a dose-dependent manner. Cotransfection of renin and ACE cDNAs stimulated further RNA synthesis as compared to ACE or renin cDNA alone. These results showed that transfected components of RAS can modulate VSMC growth through the endogenous production of vascular angiotensin II, and that ACE as well as renin are rate limiting in determining the VSMC RAS activity. We conclude that the hemagglutinating virus of Japan liposome-mediated gene transfer technique provides a new and useful tool for study of endogenous vascular modulators such as vascular RAS.
Woman loses fight for parents' right to know of abortion advice
Authors: Clare Dyer
Journal: BMJ (2006)

A mother of two teenage daughters this week lost her legal battle in the High Court in London for parents' right to know if girls under 16 are being advised on abortion.

Mr Justice Silber ruled that parents' rights under human rights legislation were over-ridden by children's right to confidentiality, if they were mature enough to understand the implications and therefore competent to take such decisions.

The ruling upholds the legality of guidance issued by the Department of Health in 2004 to try to stem the rise in teenage pregnancies and sexually transmitted diseases. This states that children should be urged to talk to their parents or …
A prospective study of the impact of laser treatment on vascular lesions
Authors: G. Gupta, D. Bilsland
Journal: British Journal of Dermatology (2000)

Background Vascular lesions, especially on exposed sites, can be unsightly and may cause significant psychological distress. Lasers are effective in treating such lesions, but relatively few studies have been performed looking at psychological scoring before and after laser therapy. Objectives To assess the change in psychological distress in patients with vascular lesions following laser treatment. Patients and methods A prospective study was performed with patients recruited over a 3-month period. Psychological distress was measured using subjective scores on a standard questionnaire before treatment and at a 6-month follow-up. Each patient was treated every 4–8 weeks with an appropriate laser by the same dermatologist until discharge. Forty-two patients were recruited with one of four diagnoses: telangiectasia, port wine stain (PWS), vascular spider or cherry angioma, which occurred mainly on facial or exposed sites. Results Following laser treatment, there was a significant decrease in subjective scores of patients with telangiectasia and vascular spiders. Patients with PWS showed objective improvement but this was not reflected in their subjective scores. Psychological distress had reduced significantly in patients with less severe vascular lesions. Conclusions Laser treatment of minor vascular lesions leads to objective improvement, which is paralleled by psychological benefit, but objective benefit in PWS may not be perceived as beneficial by patients.
Distribution of gastrointestinal hormones in the adaptive response after small bowel transplantation.
Authors: E Deltz, J H Gebhardt, C Preissner, P Schroeder, M L Hansmann, E Kaiserling, H K Muller-Hermelink, A Thiede
Journal: Gut (1987)

Enteroendocrine regulatory peptides may play an important role in the adaptation of small bowel mucosa, and it is likely that they act interdependently with neural and luminal stimuli. We assessed their action in rats by morphometric evaluation of enteroendocrine cells after heterotopic accessory small bowel transplantation (SBT), in which the graft is shunted off from the intestinal passage and is entirely deprived of neural connections, and after orthotopic SBT with normal intestinal passage. Sections of the jejunum and the ileum of the graft were immunostained with antibodies to cholecystokinin (CKK), neurotensin (NT) and vasoactive intestinal peptide (VIP). The amount and distribution of positive cells was assessed semiquantitatively. Three weeks after SBT in the heterotopic graft there was a marked decrease in CCK and NT positive cells and VIP positive fibres in the entire organ, compared with the controls. Histological examination revealed that villi and crypts had atrophied. After orthotopic SBT the number of CCK and NT positive cells increased and exceeded normal values by 20-40%. VIP positive fibres did not reach normal amounts. No mucosal atrophy was detected. These findings support the view that the intrinsic neurohormonal system is reestablished in the grafted small bowel and that enteroendocrine regulatory peptides may act as trophic factors that are responsible for adaptation after SBT.
High prevalence of Neisseria meningitidis hypervirulent lineages and emergence of W135:P1.5,2:ST-11 clone in Southern Brazil
Authors: Luciana Weidlich, Ludmila F. Baethgen, Leonard W. Mayer, Camile Moraes, Cecília C. Klein, Luciana S. Nunes, Sílvia da S. Rios, Claudete I. Kmetzsch, Maria L.R. Rossetti, Arnaldo Zaha
Journal: Journal of Infection (2008)
"Crisis" in Chinese
Authors: L. K. HSU
Journal: Annals of Internal Medicine (1974)

Comments1 March 1974"Crisis" in ChineseL. K. HSU, M.B., M.R.C.psych.L. K. HSU, M.B., M.R.C.psych.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-80-3-422_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: The recent article, "Psychiatry and Medicine, 1973," by Dr. John Romano (Ann Intern Med 79:582-588, 1973), mentioned that the word "crisis" in Chinese was written in two characters, one meaning "danger," the other, "opportunity." Having taken the two words apart, he then proceeded to say, "Our opportunity is to examine our role. . . . "I have nothing against Dr. Romano's style of writing, however, I think what he said showed a lack of understanding of the Chinese language.It is true that "crisis" in Chinese is made up of two characters. The first can be translated... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Department of Psychological Medicine Hospital For Sick Children Great Ormond Street London WC1, England PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited BySynthesis of cyanopyridine and pyrimidine analogues as new anti-inflammatory and antimicrobial agents 1 March 1974Volume 80, Issue 3Page: 422-422 Issue Published: 1 March 1974 PDF DownloadLoading ...
Diffuse Thymic Enlargement in Hodgkin's Disease
Authors: Norma E. Tartas
Journal: JAMA (1985)

DIFFUSE thymic hyperplasia following chemotherapy has rarely been reported in Hodgkin's disease, but its presence has been proposed as a favorable sign for the control of the illness.<sup>1</sup> We report herein a case of a 33-year-old woman with mixed cellular Hodgkin's disease who went into complete remission after chemotherapy and radiotherapy. While she was in complete remission, a mediastinal mass appeared that was proven histologically to be an enlarged thymus. <h3>Report of a Case</h3> A 33-year-old woman had been healthy until 1979, when she started to suffer from intermittent fever and night sweats. In August 1980, a physical examination detected left laterocervical, supraclavicular, and right axillar lymphadenopathies. The diagnosis of stage IIB mixed cellular Hodgkin's disease was made by a biopsy of the supraclavicular node and by staging procedures. She was treated with chemotherapy (cyclophosphamide, vincristine, procarbazine, and prednisone) and radiotherapy (total nodal irradiation).<sup>2</sup>She went into complete
Abstract 4334: Tracking of leukemic stem cells from frozen and fresh samples in a mouse model of human AML using bioluminescent imaging
Authors: Amanda Graham, Robert Stuart, Amanda LaRue, Michael Rosol
Journal: Cancer Research (2010)

Abstract The aim of this study was to develop and use in vivo bioluminescent imaging to track luciferase-labeled acute myelogenous leukemia (AML) stem cells in immunedeficient mice using AML blood samples collected from patients and subsequently frozen. The ability to utilize frozen pools as viable reservoirs for AML stem cells will allow the freezing back of samples from the clinic for later use as well as the use of previously frozen samples for engraftment and therapeutic studies. Using thawed, isolated cells, we assessed AML stem cell engraftment and quantitatively compared it to fresh AML stem cell engraftment in a xenogeneic transplant model using in vivo bioluminescent imaging. Frozen AML stem cell engraftment was also compared to both fresh and frozen normal human hematopoietic stem cell (HSC) engraftment. Human hematopoietic stem cells (CD34+CD38-) were isolated from both normal frozen cord blood and from peripheral blood of AML patients using modified Miltenyi Biotec (Auburn, CA) magnetic bead isolation kits. Isolated cells were transduced with a lentiviral vector (HIV-luc) pseudotyped with the VSV envelope able to express the firefly luciferase (fluc) gene in human HSC. Using a Caliper Life Sciences/Xenogen IVIS 200 optical imaging system, cells were assayed for fluc expression 48 hours after transduction. Transduced HSC and AML cells (6 × 104 each) were transplanted intravenously into sublethally irradiated adult NOD/SCID/IL2Rγnull mice and mice were imaged serially from day 1 up to day 160 post transplantation. Observed bioluminescent signal and patterns from thawed AML stem cell engraftment are similar to our results obtained using fresh AML stem cells in mice. Luminescent signal from peripheral blood and spleen engraftment of frozen AML stem cells was detectable within one day post-transplantation. Both fresh and frozen AML stem cell populations exhibited diffuse whole body signal, indicating luciferase-labeled stem cells in the peripheral blood, similar to a hallmark of the disease in humans. The presence of blast cells consistent with AML disease was observed by blood smears taken from mice engrafted with frozen AML stem cells. To our knowledge, we have demonstrated for the first time that frozen AML stem cells can be efficiently labeled with a luciferase enzyme for dynamic tracking in vivo. This work has resulted in a useful animal model that will enable us to elucidate the engraftment patterns of AML cells in living animals, learn the dynamics of the interplay of different stem cell populations, and provide information on the efficacy of new treatments in the future. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4334.
DISPARITIES IN ASSOCIATION OF VARIOUS LIPID RATIOS ON THE LOCATION AND SEVERITY OF ATHEROSCLEROSIS IN DIFFERENT VASCULAR BEDS
Authors: Palaniappan Manickam, Ankit Rathod, Apurva Badheka, Pawan Hari, Vikas Veeranna, Jyotiranjan Pradhan, Sony Jacob, Luis Afonso
Journal: Journal of the American College of Cardiology (2010)
Does B-Type Natriuretic Peptide Testing Affect Outcome and Management of Patients With Acute Dyspnea?
Authors: Mihály Tapolyai
Journal: Annals of Internal Medicine (2009)

Letters18 August 2009Does B-Type Natriuretic Peptide Testing Affect Outcome and Management of Patients With Acute Dyspnea?Mihály Tapolyai, MDMihály Tapolyai, MDFrom Louisiana State University Health Sciences Center—Shreveport, Shreveport, LA 71103-4228.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-151-4-200908180-00019 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:Schneider and colleagues (1) describe how a group of Australian emergency department physicians treat patients with severe shortness of breath. They evaluate their patients on the basis of their symptoms and promptly institute therapy based on their clinical impression.Their study examined whether advising physicians that “a BNP [B-type natriuretic peptide] level less than 100 ng/L made the diagnosis of heart failure unlikely, whereas a BNP level greater than 500 ng/L made heart failure likely,” would alter management of those patients (1). No other decision methodology, tree, or algorithm was instituted. The results show no difference between ...Reference1. Schneider HG, Lam L, Lokuge A, Krum H, Naughton MT, De Villiers Smit P, et al. B-type natriuretic peptide testing, clinical outcomes, and health services use in emergency department patients with dyspnea: a randomized trial. Ann Intern Med. 2009;150:365-71. [PMID: 19293069] LinkGoogle Scholar Author, Article, and Disclosure InformationAffiliations: From Louisiana State University Health Sciences Center—Shreveport, Shreveport, LA 71103-4228.Disclosures: None disclosed. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoB-Type Natriuretic Peptide Testing, Clinical Outcomes, and Health Services Use in Emergency Department Patients With Dyspnea Hans-Gerhard Schneider , Louisa Lam , Amaali Lokuge , Henry Krum , Matthew T. Naughton , Pieter De Villiers Smit , Adam Bystrzycki , David Eccleston , Jacob Federman , Genevieve Flannery , and Peter Cameron Does B-Type Natriuretic Peptide Testing Affect Outcome and Management of Patients With Acute Dyspnea? Ajay Pachika , Malek Numeir , and Kiranmayi Adimoolam Does B-Type Natriuretic Peptide Testing Affect Outcome and Management of Patients With Acute Dyspnea? Franca Dipaola , Francesca Perego , Monica Solbiati , Giovanni Casazza , and Does B-Type Natriuretic Peptide Testing Affect Outcome and Management of Patients With Acute Dyspnea? Hans-Gerhard Schneider , Louisa Lam , and Peter Cameron Metrics 18 August 2009Volume 151, Issue 4Page: 288KeywordsBehaviorBrain natriuretic peptidesConflicts of interestDecision treesDyspneaHeart failureLongitudinal studiesNatriuretic peptidesPatients ePublished: 18 August 2009 Issue Published: 18 August 2009 CopyrightCopyright © 2009 by American College of Physicians. All Rights Reserved.PDF DownloadLoading ...
REPORT OF FIFTEEN CASES OF PUERPERAL ECLAMPSIA.Read in the Section of Obstetrics and Diseases of Women, at the Forty-first Annual Meeting of the American Medical Association, Nashville, Tenn., May, 1890.
Authors: JOHN G. MEACHEM
Journal: JAMA (1890)

There is hardly a subject connected with obstetrics, or medical practice, that has been spoken and written upon so much as puerperal eclampsia, and yet the profession seems to be as unsettled in regard to its treatment, as it was forty years ago. One practitioner adheres to the long taught plan of bleeding by venesection, another pins his faith upon opium, chloral, chloroform, veratrum, etc. The first discards opiates and narcotics, as a rule, while the other would think it almost criminal to open a vein. Success has without doubt attended both modes of practice, but there are cases where the exclusive adoption of either, would prove disastrous. I propose to give to this Section the history of a number of cases, fifteen in all, that have come under my own observation and management, where venesection has been resorted to in all of them but one, and the favorable results
Aldosterone in Urine of Normal Man and of Patients with Oedema
Authors: B. J. Axelrad, J. E. Cates, B. B. Johnson, J. A. Luetscher
Journal: BMJ (1955)

HAEMOLYTIC DISEASE OF NEWBORN MEDICJOURNALperfusion has to be repeated will be influenced by the speed with which the liver develops the capacity to excrete bilirubin.Because of the underlying hepatic deficit in the new- born baby the importance of using recently drawn blood for transfusion is stressed.The significance of the large amounts of direct-reacting bile pigment that have been shown to occur in the plasma of some cases of haemolytic disease is discussed.This study has been made possible by the close co-operation of Miss M. Ashforth in following the clinical condition of the infants and in securing specimens for analysis.She has also been responsible for most of the exchange transfusions.I am grateful to Dr. A. White Franklin for opportunities of studying infants under his care.The serological and haematological ex- aminations have been supervised by Dr. John Murray.
How do the EQ-5D, SF-6D and the well-being rating scale compare in patients with ankylosing spondylitis?
Authors: A. Boonen, D. van der Heijde, R. Landewe, A. van Tubergen, H. Mielants, M. Dougados, S. van der Linden
Journal: Annals of the Rheumatic Diseases (2007)

To compare aspects of validity of EuroQol-5 Dimensions (EQ-5D) and Short-Form-6 Dimensions (SF-6D), two indirect utility instruments, and the well-being rating scale (RS) in ankylosing spondylitis (AS).EQ-5D, SF-6D and RS were available for 254 patients fulfilling modified New York criteria. 134 patients were part of an observational cohort and 120 were part of a randomised controlled trial (RCT). Aspects of validity assessed were truth (agreement and correlation with external health measures) and discrimination (differentiation between health states, repeatability and detection of treatment effect).Median (range) values were 0.69 (-0.08-1.00) for the EQ-5D, 0.65 (0.35-0.95) for the SF-6D and 0.65 (0.14-1.00) for the RS. Agreement (intraclass correlation coefficient) was moderate (0.46-0.55). Instruments correlated equally with disease activity, functioning and quality of life. The SF-6D showed smaller average differences in utility between patients with better and worse disease compared with the EQ-5D and the RS. The smallest detectable difference (SDD) (in the control group of RCT) was 0.36, 0.17 and 0.33 for EQ-5D, SF-6D and RS, respectively. The ability to detect treatment effect (in the intervention trial) showed standardised effect sizes that were moderate for EQ-5D and SF-6D (0.63 and 0.64) and low for the RS (0.23).In patients with AS, EQ-5D, SF-6D and the RS correlate equally well with external measures of health, but have different psychometric properties. The SDD is most favourable for the SF-6D, but it discriminates less well between patients with different disease severities. The RS has a poorer ability to detect treatment effects. It is difficult to recommend one of the instruments.
MeRIP-PF: An Easy-to-Use Pipeline for High-Resolution Peak-Finding in MeRIP-Seq Data
Authors: Yuli Li, Shuhui Song, Cuiping Li, Jun Yu
Journal: Genomics Proteomics & Bioinformatics (2013)

Abstract RNA modifications, especially methylation of the N6 position of adenosine (A)—m6A, represent an emerging research frontier in RNA biology. With the rapid development of high-throughput sequencing technology, in-depth study of m6A distribution and function relevance becomes feasible. However, a robust method to effectively identify m6A-modified regions has not been available yet. Here, we present a novel high-efficiency and user-friendly analysis pipeline called MeRIP-PF for the signal identification of MeRIP-Seq data in reference to controls. MeRIP-PF provides a statistical P-value for each identified m6A region based on the difference of read distribution when compared to the controls and also calculates false discovery rate (FDR) as a cut off to differentiate reliable m6A regions from the background. Furthermore, MeRIP-PF also achieves gene annotation of m6A signals or peaks and produce outputs in both XLS and graphical format, which are useful for further study. MeRIP-PF is implemented in Perl and is freely available at http://software.big.ac.cn/MeRIP-PF.html.
Targeting the Urokinase Plasminogen Activator Receptor Inhibits Ovarian Cancer Metastasis
Authors: Hilary A. Kenny, Payton Leonhardt, Andras Ladanyi, S. Diane Yamada, Anthony Montag, Hae Kyung Im, Sujatha Jagadeeswaran, David E. Shaw, Andrew P. Mazar, Ernst Lengyel
Journal: Clinical Cancer Research (2010)

To understand the functional and preclinical efficacy of targeting the urokinase plasminogen activator receptor (u-PAR) in ovarian cancer.
Work of 125 aid agencies failed to create lasting rehabilitation services in Haiti, study shows
Authors: S. Arie
Journal: BMJ (2012)

Two years after the devastating earthquake that left over 300 000 people injured in Haiti, international aid agencies have failed to work with the government to create rehabilitation services that will last, a study has concluded.

“Thanks to the earthquake, for the first time this country has proper rehabilitation services,” said Karl Blanchet, a coauthor of the study. “But the quality depends on the international organisations. If they leave, the services will go too.” The study was conducted by a team at the International Centre for Evidence in Disability at the London School of Hygiene and Tropical Medicine.

Haiti received a far greater rehabilitation response after the January 2010 earthquake than previous disasters such as the 2004 Indian Ocean tsunami, possibly because of its proximity to the United States. But in many ways the sheer number of organisations involved in rehabilitation alone (125, including United Nations agencies and government, international, and Haitian non-governmental organisations) led …