tbuckley/cabot-search huggingface dataset:
16. Direct Selection of Targeted Vector from a Random Peptide Library Displayed on Adenoviral Fiber Knob
Authors: Kazunori Aoki, Yoshiaki Miura, Kimiko Yoshida, Kazuteru Hatanaka, Takeshi Nishimoto, Shumpei Ohnami, Joanne T. Douglas, David T. Curiel, Teruhiko Yoshida
Journal: Molecular Therapy (2006)

Recombinant adenovirus vectors have been widely used for gene delivery to a range of cell types and employed in a number of gene therapy approaches. However, a selective delivery of a therapeutic gene to target cells by adenovirus vectors is precluded by the widespread distribution of the primary cellular receptors for adenoviruses. Recently, retargeting adenovirus vectors has been achieved by direct genetic modifications of the capsid proteins: targeting ligands can be incorporated into the C-terminal and HI-loop of fiber proteins ablated for native tropism through loss of binding with CAR and av integrins, and these vectors provide an important platform for evaluating the targeting potential of selected peptide ligands. However, cell-type specific lligand-receptor systems for targeted adenovirus vectors are generally unknown, which impedes the wider application of fiber-modified adenovirus vectors for targeted therapies. Moreover, because the insertion of a pre-selected peptide into a fiber knob often fails to generate an adenovirus vector as shown in this study, the selection of targeting peptides is highly useful in the context of the adenoviral capsid. We reported previously a simple and efficient method for constructing an adenovirus cDNA expression library by in vitro Cre-loxP recombination (K. Hatanaka, et al. Mol Ther 8;158, 2003). Based on that technology, here we developed a novel system for adenoviral libraries which display a wide variety of peptides on the HI-loop of the fiber knob. A plasmid-based library containing a GFP clone at an abundance of 0.01% was readily converted to the adenovirus library in a convenient 60-mm dish scale, suggesting that the complexity of the virus library could be more than 1 × 104 per a 60-mm dish by the present method. As proof-of-concept, we screened the 2 × 105 complexity level of adenovirus display library on a human gliomacell line and observed selection of a particular peptide sequence. The gene transduction efficiency of a targeted vector carrying the peptide was 4- to 5-fold higher than that of an untargeted adenovirus in the glioma cell line but not in other cell lines. A substantial competitive inhibition by the cognate peptide but not by a control unrelated peptide was observed, confirming that the enhanced transduction of the targeted vector in the cells is mediated by the insertion of the selected peptide. Our system may allow the selection of specific targeted vectors to any given cell type of interest, and may have broad implications for the development of targeted therapies.
MRI bone oedema is the strongest predictor of subsequent radiographic progression in early rheumatoid arthritis. Results from a 2-year randomised controlled trial (CIMESTRA)
Authors: M L Hetland, B Ejbjerg, K Hørslev-Petersen, S Jacobsen, A Vestergaard, A G Jurik, K Stengaard-Pedersen, P Junker, T Lottenburger, I Hansen, L S Andersen, U Tarp, H Skjødt, J K Pedersen, O Majgaard, A J Svendsen, T Ellingsen, H Lindegaard, A F Christensen, J Vallø, T Torfing, E Narvestad, H S Thomsen, M Østergaard
Journal: Annals of the Rheumatic Diseases (2008)

Objective: To identify predictors of radiographic progression in a 2-year randomised, double-blind, clinical study (CIMESTRA) of patients with early rheumatoid arthritis (RA). Methods: Patients with early RA (n = 130) were treated with methotrexate, intra-articular betamethasone and ciclosporin/placebo-ciclosporin. Baseline magnetic resonance imaging (MRI) of the wrist (wrist-only group, n = 130) or MRI of wrist and metacarpophalangeal (MCP) joints (wrist+MCP group, n = 89) (OMERACT RAMRIS), x -ray examination of hands, wrists and forefeet (Sharp/van der Heijde Score (TSS)), Disease Activity Score (DAS28), anti-cyclic citrullinated peptide antibodies (anti-CCP), HLA-DRB1-shared epitope (SE) and smoking status were assessed. Multiple regression analysis was performed with delta-TSS (0–2 years) as dependent variable and baseline DAS28, TSS, MRI bone oedema score, MRI synovitis score, MRI erosion score, anti-CCP, smoking, SE, age and gender as explanatory variables. Results: Baseline values: median DAS28 5.6 (range 2.4–8.0); anti-CCP positive 61%; radiographic erosions 56%. At 2 years: DAS28 2.0 (0.5–5.7), in DAS remission: 56%, radiographic progression 26% (wrist+MCP group, similar for wrist-only group). MRI bone oedema score was the only independent predictor of delta-TSS (wrist+MCP group: coefficient = 0.75 (95% CI 0.55 to 0.94), p&lt;0.001; wrist-only group: coefficient = 0.59 (95% CI 0.40 to 0.77), p&lt;0.001). Bone oedema score explained 41% of the variation in the progression of TSS (wrist+MCP group), 25% in wrist-only group (Pearson’s r = 0.64 and r = 0.50, respectively). Results were confirmed by sensitivity analyses. Conclusion: In a randomised controlled trial aiming at remission in patients with early RA, baseline RAMRIS MRI bone oedema score of MCP and wrist joints (and of wrist only) was the strongest independent predictor of radiographic progression in hands, wrists and forefeet after 2 years. MRI synovitis score, MRI erosion score, DAS28, anti-CCP, SE, smoking, age and gender were not independent risk factors. Trial registration number: NCT00209859.
Piroxicam induced submassive necrosis of the liver.
Authors: D Paterson, P Kerlin, N Walker, S Lynch, R Strong
Journal: Gut (1992)

Several widely used non-steroidal anti-inflammatory drugs have been reported as causing severe hepatitis. Three cases of severe acute hepatitis have been reported in association with piroxicam. A fatal submassive necrosis that occurred in a 68 year old lady who had received piroxicam for 15 months is described. A 48 year old man who developed submassive hepatic necrosis six weeks after beginning piroxicam but was successfully treated with orthotopic liver transplantation is also reported. Piroxicam may induce submassive necrosis of the liver, probably as an idiosyncratic reaction.
A common variant of HMGA2 is associated with adult and childhood height in the general population
Authors: Michael N Weedon, Guillaume Lettre, Rachel M Freathy, Cecilia M Lindgren, Benjamin F Voight, John R B Perry, Katherine S Elliott, Rachel Hackett, Candace Guiducci, Beverley Shields, Eleftheria Zeggini, Hana Lango, Valeriya Lyssenko, Nicholas J Timpson, Noel P Burtt, Nigel W Rayner, Richa Saxena, Kristin Ardlie, Jonathan H Tobias, Andrew R Ness, Susan M Ring, Colin N A Palmer, Andrew D Morris, Leena Peltonen, Veikko Salomaa, George Davey Smith, Leif C Groop, Andrew T Hattersley, Mark I McCarthy, Joel N Hirschhorn, Timothy M Frayling
Journal: Nature Genetics (2007)
No Greater Love
Authors: Melanie S. Wayne
Journal: JAMA (1988)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
811 ESTIMATING THE LIKELIHOOD OF SUSTAINED VIROLOGICAL RESPONSE IN CHRONIC HEPATITIS C (CHC) THERAPY
Authors: S. Mauss, D. Hueppe, C. John, J. Goelz, U. Meyer, R. Heyne, B. Moeller, R. Link, G. Teuber, S. Sworszt, A. Herrmann, M. Spelter, A. Wollschlaeger, H. Steffens, A. Baumgarten, K.G. Simon, N. Dikopoulos, T. Witthoeft
Journal: Journal of Hepatology (2008)
Is Diphenylhydantoin Effective in Treatment of Alcohol Withdrawal?
Authors: Peter K. Gessner
Journal: JAMA (1972)

<h3>To the Editor.—</h3> Finer (<b>217</b>:211, 1971) and Adams (<b>218</b>:598, 1971) believe the concern I expressed (<b>216</b>:887,1971) regarding the use of diphenylhydantoin for the treatment of alcohol withdrawal is unwarranted. I am unconvinced. Available evidence does not suggest, in my view, that diphenylhydantoin by itself can prevent or control the seizures and other symptoms of alcohol withdrawal. Given this dichotomy of opinions and its potential therapeutic significance, a review of the facts is warranted. While pharmacology textbooks<sup>1,2</sup>attest to the ability of diphenylhydantoin to control grand mal seizures in the epileptic, they remain silent regarding its effectiveness in controlling the seizures of alcohol withdrawal. The<i>Medical Letter on Drugs and Therapeutics</i><sup>3</sup>comments that there are no well-controlled trials of diphenylhydantoin in the treatment of alcohol withdrawal and the<i>AMA Drug Evalvations</i>,<sup>4</sup>as Adams notes, states "the hydantoin are usually of little value" in preventing "these dangerous
Leukocyte Esterase Tests Detect Pyuria, Not Bacteriuria
Authors: Edward J. Boyko
Journal: Annals of Internal Medicine (1993)

Letters1 February 1993Leukocyte Esterase Tests Detect Pyuria, Not BacteriuriaEdward J. Boyko, MDEdward J. Boyko, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-118-3-199302010-00017 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:The spectrum bias described recently by Lachs and associates[1] with regard to the rapid dipstick test for UTI may be due in whole or in part to the effects of the imperfect reference test for UTI chosen as their “gold standard.” The criterion for a positive urine culture of greater than 105 colony-forming units (CFU)/mL is not 100% sensitive and specific for UTI, as one expects of an ideal “gold standard” that accurately separates those with and those without disease.Use of an imperfect reference test in the evaluation of diagnostic test sensitivity and specificity has predictable ...References1. Lachs MS, Nachamkin I, Edelstein PH, Goldman J, Feinstein AR, Schwartz JS. Spectrum bias in the evaluation of diagnostic tests: lessons from the rapid dipstick test for urinary tract infection. Ann Intern Med. 1992; 117:135-40. Google Scholar2. Boyko EJ, Alderman BW, Baron AE. Reference test errors bias the evaluation of diagnostic tests for ischemic heart disease. J Gen Intern Med. 1988; 3:476-81. Google Scholar3. Schulzer M, Anderson DR, Drance SM. Sensitivity and specificity of a diagnostic test determined by repeated observations in the absence of an external standard. J Clin Epidemiol. 1991; 44:1167-79. Google Scholar Author, Article, and Disclosure InformationAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byDiagnostic test accuracy may vary with prevalence: implications for evidence-based diagnosis 1 February 1993Volume 118, Issue 3Page: 230-231KeywordsColony forming unitsEsterasesSpecificityUrine Issue Published: 1 February 1993 Copyright & PermissionsCopyright © 1993 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Assessment of patients with suspected viral haemorrhagic fever.
Authors: R T Emond, H Smith, P D Welsby
Journal: BMJ (1978)

We reviewed our two-year (1967-7) experience of patients who might have had an African-derived haemorrhagic fever: only two out of 46 suspects had such infections, and these were successfully contained. Malaria was the most frequent diagnosis in these patients recently returned from Africa, especially if malarial prophylaxis had been inadequate. Altogether 26% of patients had become ill before travelling to Britain. It is impossible to ensure absolute safety even with draconian measures, but the degree of isolation recommended (based on epidemiological factors that included return from Africa within the previous three weeks, visiting country districts, exposure to possible cases, or infected material and illness despite adequate malarial prophylaxis) has proved effective and acceptable.
Gastric emptying in control subjects and patients with duodenal ulcer before and after vagotomy.
Authors: P J Howlett, H J Sheiner, D C Barber, A S Ward, C A Perez-Avila, H L Duthie
Journal: Gut (1976)

The emptying of a solid meal labelled with Indium 113mDTPA from the stomach was studied with a gamma camera in 26 normal subjects, 27 patients with duodenal ulcer, on 41 occasions after truncal vagotomy and pyloroplasty and 38 times after highly selective vagotomy. Applying the method of principal component analysis to the results, differences were detected between control and duodenal ulcer subjects and two probable subgroups of duodenal ulcer were observed. Half emptying times did not reveal these patterns. After vagotomy, delayed emptying was general at one week. At one month, patients after highly selective vagotomy had a more normal result than those with truncal vagotomy and pyloroplasty (TV), but by six months no significant difference in overall emptying rate was found, although changes in the pattern of gastric emptying persisted in some patients after TV.
Antibody‐induced von willebrand syndrome: Inhibition of VIII VWF and VIII AGN with sparing of VIII AHF by the autoanti body
Authors: C. Gazengel, C. Jacques, J. Nedellec, F. Josso, D. Buriot, A. M. Prieur
Journal: American Journal of Hematology (1978)

Abstract Acquired von Willebrand syndrome is reported in a 16‐year‐old girl with systemic lupus erythematosus. Routine coagulation studies showed a normal platelet count, prolonged bleeding time, and abnormal glass bead retention. Factor VIII molecular complex respective activities were 8% for VIII AHF and undetectable for VIII VWF (ristocetin aggregation of washed platelets) and VIII AGN (electro‐immunodiffusion). In vitro, the patient's plasma exhibited an inhibitory activity against exogenous VIII AGN and VIII VWF but did not neutralize VIII AHF activity of control plasma, even after a two‐hour incubation at 37°C. This inhibitory activity was supported by the purified plasma IgG fraction. In vivo, following cryoprecipitate administration (20 units VIII AHF/kg), only 50% of the infused VIII AHF activity was recovered after 15 mn, and the original level was reached four hours later. Only a transient peak of VIII VWF activity was observed, and VIII AGN level did not increase at all after the infusion. After the start of prednisone therapy the three factor VIII related activities returned to normal levels in the following order: VIII AHF and VIII VWF (9‐12 days); VIII AGN (3 weeks). These findings could be explained by the formation of a short‐lived circulating immune complex between the antibody and the factor VIII molecular complex. In such an hypothesis the autoantibody would respect the site of VIII AHF activity and would mask the site reacting with anti VIII AGN heteroantibodies.
Integrelin for emergency coronary artery stenting
Authors: James P. Zidar, Kevin R. Kruse, Mark C. Thel, Dean Kereiakes, Joseph B. Muhlestein, Charles J. Davidson, Paul S. Teirstein, Alan Tenaglia, Steven J. Yakubov, Jeffrey J. Popma, Jean-Francois Tanguay, Michael M. Kitt, Todd J. Lorenz, James E. Tcheng, A.Michael Lincoff, Robert M. Calliff, Eric J. Topol
Journal: Journal of the American College of Cardiology (1996)
Hospital group admits that its premises were used for illegal transplantations
Authors: P. Sidley
Journal: BMJ (2010)

The largest of South Africa’s three private hospital groups, Netcare, has admitted in court that St Augustine’s Hospital, Durban, which is owned by a Netcare subsidiary, was used for over 100 illegal kidney transplantations.

The illegal operations, which were carried out between June 2001 and November 2003, involved transplanting healthy kidneys from Brazilian and Romanian donors into Israelis needing kidneys but who were unable to obtain them in Israel under Israeli law. 

St Augustine’s Hospital is owned by Netcare KwaZulu-Natal (NKZ), a wholly owned subsidiary of Netcare.

A government spokesman, Themba Maseko, has said that investigations are continuing.

Netcare, which also operates in the United Kingdom, has been fined 4m rand (£0.36m; €0.42m; $0.6m) for its role …
Doppler Estimation of Left Ventricular Filling Pressure in Sinus Tachycardia
Authors: Sherif F. Nagueh, Issam Mikati, Helen A. Kopelen, Katherine J. Middleton, Miguel A. Quiñones, William A. Zoghbi
Journal: Circulation (1998)

Background —Doppler echocardiography is frequently used to predict filling pressures in normal sinus rhythm, but it is unknown whether it can be applied in sinus tachycardia, with merging of E and A velocities. Tissue Doppler imaging (TDI) can record the mitral annular velocity. The early diastolic velocity (E a ) behaves as a relative load-independent index of left ventricular relaxation, which corrects the influence of relaxation on the transmitral E velocity. Methods and Results —We evaluated 100 patients 64±12 years old with simultaneous Doppler and invasive hemodynamics. Mitral inflow was classified into 3 patterns: complete merging of E and A velocities (pattern A), discernible velocities with A dominance (B), or E dominance (C). The Doppler data were analyzed at the mitral valve tips for E, acceleration and deceleration times of E, and isovolumic relaxation time. In patterns B and C, the A velocity, E/A ratio, and atrial filling fraction were derived. Pulmonary venous flow velocities were also measured, and TDI was used to acquire E a and A a . Weak significant relations were observed between pulmonary capillary wedge pressure (PCWP) and sole parameters of mitral flow, pulmonary venous flow, and annular measurements. These were better for patterns A and C. E/E a ratio had the strongest relation to PCWP [ r =0.86, PCWP=1.55+1.47(E/E a )], irrespective of the pattern and ejection fraction. This equation was tested prospectively in 20 patients with sinus tachycardia. A strong relation was observed between catheter and Doppler PCWP ( r =0.91), with a mean difference of 0.4±2.8 mm Hg. Conclusions —The ratio of transmitral E velocity to E a can be used to estimate PCWP with reasonable accuracy in sinus tachycardia, even with complete merging of E and A velocities.
Laboratory Tests in Rheumatic Diseases: Standardisation in Laboratory and Clinical Practice
Authors: P. H. N. Wood
Journal: Annals of the Rheumatic Diseases (1980)

chapter on rehabilitation aspects, but the sophisticated and annotated approach in both of them tends to obscure the simplicity of such measures as static quadriceps drill and wrist extension exercises, which to an informed patient will make-at the cost of 5 minutes of the physician's time-all the difference between a useful limb and functional incapacity.But these and one or two other points of detail are only minor criticisms of an exhaustive textbook.Exhaustive it must have been.Joe Hollander and I compared notes (I had recently edited the most recent edition of the book's British counterpart) while we cruised up the Rhine together during the Wiesbaden conference of September 1979.'You know, Tom', he reflected, 'I reckon that producing an edition of a book like this takes 2 years out of your life'.I agreed in principle, though admittedly never having considered the matter in actuarial terms.British rheumatologists will also agree with me in congratulating Dr McCarty and his many colleagues on the fruits of their labours, yet another edition of almost impeccable excellence.
Reexamination of the need for treatment in idiopathic thrombocytopenic purpura
Authors: Michael Auerbach, Harold Ballard
Journal: American Journal of Hematology (2006)

A recent article in Blood, ''How I Treat Idiopathic Thrombocytopenic Purpura (ITP)'' by Cines and Bussel, was read with great interest [1].Placing in perspective the new treatment modalities is useful to all hematologists, yet the only published data the authors cited to support their recommendations were a meta-analysis of patients with platelet counts below 30,000, which did not differentiate between treated and untreated patients.There are no publications extant that suggest that treatment-naive, otherwise healthy adult ITP patients with any platelet count are at risk for significant bleeding.In 1988, Brox et al. showed a significant rise in the platelet count with high doses of ascorbic acid in refractory ITP patients [2].Subsequent to Brox's report a study of ascorbic acid for the treatment of refractory ITP was published by Jubilirer [3], which failed to confirm Brox's data; however, it is to be noted that no bleeding episodes occurred in the treatment arm.In 1983 Burns and Saleem [4] published a review of the natural history of refractory ITP patients (steroid and splenectomy failures) and concluded that significant hemorrhagic events were extremely rare.In 2001 Vianelli et al. [5] reported on the long-term follow-up of 310 patients with ITP and concluded that ''prospective studies are required to evaluate whether it may be reasonable to treat only symptomatic patients independently of age.''Since 1988 (Auerbach), steroids have not been used in any of more than 40 patient referrals irrespective of platelet counts without thrombocytopenia-related morbidity.For platelet counts > 10,000, vitamin C alone was prescribed and bruising was virtually eliminated.This result is not unexpected inasmuch as improvement in capillary fragility and wound healing are well-recognized salutary effects with vitamin C. For counts < 10,000 WinRho and/or Rituxan has been universally successful.Therefore, it may not be unreasonable to suggest that our conventional approach to managing ITP in otherwise healthy adults (corticosteroids/ splenectomy) must be reexamined.Even in the pre IVIG, WinRho, and Rituxan era, bleeding was rarely encountered during splenectomies in ''refractory'' ITP patients.Our most important admonition, ''before else, do no harm,'' should be heeded before recommending potentially toxic therapies.
Dobutamine Stress Echocardiography for Risk Stratification of Patients With Low-Gradient Severe Aortic Stenosis Undergoing TAVR
Authors: Salim Hayek, Philippe Pibarot, Arash Harzand, Jeh-Wei Cheng, Hawkins Gay, Christina Chrysohoou, Henrique Ribeiro, Josep Rodés-Cabau, Vasilis Babaliaros, Stamatios Lerakis
Journal: JACC. Cardiovascular imaging (2014)