tbuckley/cabot-search huggingface dataset:
Abstract 5026: Loss of microRNA-143/145 disturbs cell growth of cancers by impairing the MDM2-p53 feedback loop
Authors: Wantao Chen, Jianjun Zhang, Qiang Sun
Journal: Cancer Research (2012)

Abstract BACKGROUND: Dysregulated microRNAs (miRNAs) play an important role in many malignant tumors, including epithelial cancers, but little is known about the molecular mechanisms and clinical significances of miRNAs dysregulation. The aim is to identify and determine the miR-143 and miR-145 how to specifically be involved in controlling MDM2 expression in a tumor tissue-specific manner. METHODS: Using real-time PCR, the expression of miR-143, miR-145 and MDM2 were evaluated and analyzed in epithelial cancer cell lines and oral cancer specimens. The different PCR-derived fragments and mutagenesis from the MDM2 mRNA 3′ UTR were inserted into the pGL3 vector to generate the luciferase reporters and the reporter activities were assayed by luciferase assay kits. The effects of overexpression of miR-143 and miR-145 on MDM2 and p53 levels were analyzed by western blot. The effects of miR-143 and miR-145 on cell proliferation and cell cycle were determined by the EdU assay and flow cytometry analysis. The effects of miR-143 and miR-145 on tumorigenicity in vivo were investigated with HNSCC cell xenograft models and the cell apoptotic rate as well as mechanism were tested by TUNEL and apoptosis-protein expression in the tumor tissues. RESULTS: The results showed that both miR-143 and miR-145, which belong to the same miRNA cluster, can negatively modulate expression of their target gene, MDM2. The miR-143 and miR-145 is post-transcriptionally activated by up-regulated p53, thereby generating a short miRNAs-MDM2-p53 feedback loop. Re-expression of these miRNAs suppresses cellular growth and triggers the apoptosis of epithelial cancer, in vitro and in vivo, by enhancing p53 activity via MDM2 turnover. Moreover, the miRNA-dependent MDM2 turnover contributes to the equilibrium of repeated p53 pulses in response to DNA damage stress. CONCLUSIONS: These findings suggest that MDM2 dysregulation caused by down-regulation of miR-143 and miR-145 contributes to epithelial cancer development and palys a key role in regulating cellular proliferation and apoptosis. Recruitment of miR-143 and miR-145 may be a reasonable strategy for treatment of epithelial cancers. KEYWORDS: miRNA-143, miRNA-145, MDM2, p53, epithelial cancers. ACKNOWLEDGMENTS: This study was supported by the National Natural Science Foundation of China (Grant No. 30973343 and 81101515), Projects of the Shanghai Science and Technology Committee (Grant No. 08JC1414400 and 10XD1402500), the Shanghai Leading Academic Discipline Project (S30206). *The first two authors contributed equally to this work. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5026. doi:1538-7445.AM2012-5026
ALDOSTERONE ANTAGONISM INCREASES POST-EXERCISE RECRUITMENT OF SYSTOLIC MYOCARDIAL MOTION OVER LEFT VENTRICULAR LATERAL WALL IN HYPERTENSIVE PATIENTS WITH DIASTOLIC HEART FAILURE
Authors: Yi-Chih Wang, Chih-Chieh Yu, Fu-Chun Chiu, Chia-Ti Tsai, Ling-Ping Lai, Juey-Jen Hwang, Jiunn-Lee Lin
Journal: Journal of the American College of Cardiology (2010)
&lt;emph type="ital"&gt;HMGA1&lt;/emph&gt;, A Novel Locus for Type 2 Diabetes Mellitus
Authors: Abhimanyu Garg
Journal: JAMA (2011)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Chronic Stress and Mortality Among Older Adults
Authors: Janice K. Kiecolt-Glaser
Journal: JAMA (1999)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Partner Notification: Can It Control Epidemic Syphilis?
Authors: J K Andrus, D W Fleming, D R Harger, M Y Chin, D V Bennett, J M Horan, G Oxman, B Olson, L R Foster
Journal: Annals of Internal Medicine (1990)

Study Objective: During 1987, the rate of syphilis among heterosexual adults in Oregon increased 159%, yet the number of cases of gonorrhea remained stable. Our study was done to evaluate why the same control measures contained gonorrhea but not syphilis in this population. Design: Exploratory case-control study. Setting: Public health clinics in Portland, and Salem, Oregon. Patients: All 146 heterosexual adults with early syphilis and all 164 heterosexual adults with acute gonorrhea reported to the public health clinics during April to July 1988. Intervention: A questionnaire was administered to all syphilis case-patients and control patients (those with gonorrhea) at the beginning of the routine, sexually transmitted disease (STD), investigative interview. Measurements and Main Results: Syphilis case-patients had contact with an average of 5.0 sex partners for whom they could not supply locating information sufficient to initiate partner notification. In contrast, patients with gonorrhea had contact with an average of 0.4 sex partners for whom they could not supply locating information (P < 0.005). The 28% (41 of 146) of syphilis case-patients who reported having exchanged sex for drugs or money accounted for 72% (527 of 729) of the unlocatable partners exposed to syphilis. In contrast, the 17% (28 of 164) of patients with gonorrhea who reported having exchanged sex for drugs or money accounted for 36% (25 of 69) of the unlocatable partners exposed to gonorrhea. Conclusions: Because patients infected with syphilis have relatively large numbers of anonymous sexual encounters, prevention strategies that supplement partner notification are urgently needed to control the syphilis epidemic among adults.
An Inhibitor of Gram-Negative Bacterial Virulence Protein Secretion
Authors: Heather B. Felise, Hai V. Nguyen, Richard A. Pfuetzner, Kathleen C. Barry, Stona R. Jackson, Marie-Pierre Blanc, Philip A. Bronstein, Toni Kline, Samuel I. Miller
Journal: Cell Host & Microbe (2008)
Changing US Health Care: A Study of Four Metropolitan Areas
Authors: Ronald Andersen
Journal: JAMA (1994)

The Reagan era began in 1980 with a policy to limit federal spending, promote competition and the free market, and encourage local control in the health services sector. The authors of this volume attempt to trace the influence of this policy in the decade of the 1980s in the four largest metropolitan areas of the country: New York, Chicago, Los Angeles, and Houston. In fact, the studies of the metropolitan areas were conducted by people other than the primary authors except for Berliner (who authored the chapter on New York): J. Karen Salmon—Chicago; E. Richard Brown and Geraldine Dallek—Los Angeles; and Hardy D. Loe, Jr, Virginia C. Kennedy, and Frank I. Moore—Houston. The plan was to examine similarities and differences in the metropolitan areas. Similar forces acting in all areas included federal efforts to increase Medicaid coverage for pregnant women and children; the growth of the uninsured population and change
Serum Albumin and Urea During States of Anxiety and Depression
Authors: Bernard Ecanow
Journal: JAMA (1973)

<h3>To the Editor.—</h3> We wish to report that automated serum chemistry data of 1,900 psychiatric outpatients we have studied confirm Dr. Albert Casey's finding of elevated serum albumin and lowered serum urea nitrogen levels among various anxiety states. The clinical chemistry data of the depressive patients in our sample also tend to support his observations of the albumin levels characteristic of that group (224:1532,1973). Statistical significances in our sample were 0.05 or less. It is important to emphasize that the techniques used in Dr. Casey's study measured only those substances present in the serum. Since a given total quantity of albumin and urea nitrogen are present in the body, fluctuations in serum levels of these substances must be accompanied by inverse fluctuations of the quantities of each bound to other body tissues. Our chemistry studies of blood drawn from anxious or agitated patients, indicated that serum albumin levels were elevated
SEX AND AGE IN MYASTHENIA GRAVIS AS CRITICAL FACTORS IN INCIDENCE AND REMISSION
Authors: Robert S. Schwab
Journal: JAMA (1953)

Since the communication on removal of the thymus as treatment for myasthenia gravis was presented by Blalock<sup>1</sup>in 1941, there have been a number of other reports on this procedure as a therapy in myasthenia gravis. Some of these have been enthusiastic as to the benefits achieved by removal of thymic tissue, particularly when it was not associated with tumor, in producing complete or partial remission in this disease. Geoffrey Keynes has reported the largest series of such operations, nearly 200. The first 100 of his patients were studied in considerable detail by Ross,<sup>2</sup>who reported that the operation benefited 86 patients. There was statistical evidence that the sooner the operation was done after the onset of the disease the more favorable the result. On the other hand, Eaton and Clagett<sup>3</sup>reported little statistical difference in remission rate between 70 patients treated by this operation and their
Challenges to Improve Coronary Heart Disease Risk Assessment
Authors: Peter W. F. Wilson
Journal: JAMA (2009)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
ADDITIONAL REPRESENTATIVES: A CONSTITUTIONAL QUESTION
Authors: Fred. J. Smith
Journal: BMJ (1912)

exaggerate the importance of his, work in showing the connexion between faulty dietetics and dental disease.I estimate that among the inhabitants of these islands there are upwards of 200 million carious teeth, and as many alveolar abscesses.By following the simple rules laid do,wn by Dr. Wallace both these diseases can be almost entirely prevented.-Iam, etc., London, W., Jan. 20th.
SYMPTOMATIC HERPES, A SEQUELA OF ARTIFICIALLY INDUCED FEVER
Authors: Stafford L. Warren, Charles M. Carpenter, Ruth A. Boak
Journal: The Journal of Experimental Medicine (1940)

1. Symptomatic herpes occurred in 190 (46.2 per cent) of 411 patients treated with fever induced by physical methods. 2. Herpes recurred in only 7, or 5.3 per cent, of 131 patients given subsequent fever treatments, suggesting that some immunity develops with the first attack. 3. An acute encephalitis-like syndrome of short duration and without sequelae developed in a group of patients with severe herpes following fever therapy. 4. A filter-passing virus, recovered from herpetic vesicles on patients treated with artificially induced fever, produced a fatal encephalitis in rabbits when inoculated intracerebrally and by corneal scarification. Intranuclear inclusion bodies were observed in corneal epithelial cells and in motor ganglion cells of the brain similar to those observed in rabbits injected with known strains of herpes virus. 5. Four strains of virus that had been recovered from herpetic vesicles appearing on patients subsequent to artificially induced fever were shown by cross-protection tests on rabbits to be immunologically related to the Frank strain of herpes virus.
Recording of heart movements from the oesophagus
Authors: R. VAS, C. D. THURSFIELD, E. T. MACKENZIE, D. C. KENNIE, A. B. HOUSTON
Journal: Cardiovascular Research (1974)

A further development of the displacement cardiograph has made possible the recording of left atrial and posterior left ventricle movements. A displacement cardiograph sensing element and an ECG electrode were built into a silicon tube to form an oesophageal probe. Records taken at various oesophageal levels have been recovered. Fourteen baboons were examined. Reproducible results of left atrial and posterior left ventricle movements were seen. The technique appears to be harmless and is easily performed.
Mucosal involvement in systemic and chronic cutaneous lupus erythematosus
Authors: S.M. BURGE, P.A. FRITH, R.P. JUNIPER, F. WOJNAROWSKA
Journal: British Journal of Dermatology (1989)

The prevalence and pattern of mucosal involvement in 121 patients with lupus erythematosus (LE) was investigated. Fifty-three patients had systemic LE (SLE) and 68 patients had chronic cutaneous LE (CCLE). Twenty-one per cent (11/53) of patients with SLE and 24% (16/68) of patients with CCLE had signs of mucosal involvement, but the pattern of involvement differed in the two groups. Nasal mucosal lesions were a feature in 2% (1/53) of patients with SLE and 9% (6/68) of patients with CCLE. Hyperkeratotic lichen planus-like plaques on buccal mucosa and the palate occurred in 9% (6/68) of patients with CCLE and 4% (2/53) of patients with SLE. Episcleritis occurred in 9% (5/53) of patients with SLE and was not seen in CCLE. Erythematous plaques on the lower eyelids were present in 6% (4/68) of patients with CCLE and these were associated with conjunctival scarring in two patients. Vulval lesions were present in 5% (2/42) of female patients with CCLE. Oral plaques may occur when the disease is relatively quiescent elsewhere. The prevalence of mucosal involvement in lupus is underestimated as the lesions may be asymptomatic.
Echocardiographic evaluation of ventricular septal aneurysms.
Authors: A R Snider, N H Silverman, N B Schiller, T A Ports
Journal: Circulation (1979)

The spontaneous closure of ventricular septal defects is frequently associated with septal aneurysm formation. In this paper we discuss the M-mode and two-dimensional echocardiographic findings in nine children with aneurysms of the ventricular septum in association with ventricular septal defects. In all patients the diagnosis was confirmed by angiography. The ventricular septal aneurysms were detected by both M-mode and two-dimensional echocardiography. With M-mode echocardiography, septal aneurysms could be recognized by a pattern of multiple systolic echoes within the right ventricle. With two-dimensional echocardiography, the protrusion of the septal aneurysm into the right ventricle could be seen from several views and the location and the relative size of the aneurysm assessed. Echocardiographic techniques useful in the detection of ventricular septal aneurysms are discussed and examples presented.
GMC's proposals for revalidation would not be accurate, economical, or fair
Authors: R. Wakeford
Journal: BMJ (2000)

EDITOR—To anyone involved in assessing medical competence, the General Medical Council's proposal for revalidation is potentially unfair and inaccurate, and very expensive.1

The proposal has two parts. The first—annual appraisal—is uncontroversial; it can be helpful.2 The second is a summative assessment every five years, aggregating the appraisals, which is reviewed by two doctors from the appraisee's field and a lay person; collectively they determine whether to recommend revalidation. This does not lead to de-registration: it acts as a sieve, seeking to identify …
Histopathological study of primary biliary cirrhosis and the effect of ursodeoxycholic acid treatment on histology progression
Authors: Claude Degott, Elie Serge Zafrani, Patrice Callard, Beverley Balkau, Renée Eug Poupon, Raoul Poupon
Journal: Hepatology (1999)

The semiquantitative histopathological analysis of the liver biopsies obtained before and after 4 years of ursodeoxycholic acid (UDCA) therapy in a cohort of primary biliary cirrhosis (PBC) patients is reported. The relationships between elementary histological lesions before treatment and their progression under therapy were assessed. At baseline, two independent groups of lesions, each of which participate in the development of fibrosis, were individualized, i.e. , florid bile duct lesions and ductopenia on one hand and lymphocytic piecemeal necrosis, ductular proliferation, and lobular necroinflammatory changes on the other hand. Four years of UDCA therapy were associated with a significant decrease in the prevalence of florid interlobular bile duct (ILBD) lesions, of epithelioid granuloma ( P &lt; .001) without any aggravation in the severity of bile duct paucity. Lobular inflammation and necrosis markedly improved ( P &lt; .001) whereas the degree of severity of the lymphocytic piecemeal necrosis and ductular proliferation at entry and at 4 years were similar. Worsening of fibrosis was observed in 14 patients (12 of them had a one grade progression) whereas stabilization was noted in 30 of the remaining patients. Severity of both the lymphocytic piecemeal necrosis and lobular inflammation and necrosis at entry was significantly associated with the progression of fibrosis. The results suggest that UDCA therapy influences the process leading to bile duct destruction. Patients with severe lymphocytic piecemeal necrosis and lobular inflammation may need additional therapeutic intervention because they have increased risk of fibrosis progression
Effect of longterm placebo controlled calcium supplementation on sigmoidal cell proliferation in patients with sporadic adenomatous polyps.
Authors: U M Weisgerber, H Boeing, R W Owen, R Waldherr, R Raedsch, J Wahrendorf
Journal: Gut (1996)

A longterm, double blind intervention trial was undertaken in patients with sporadic adenoma treated by polypectomy to investigate the putative role of calcium as a protective factor in colon carcinogenesis. The aim of the study was to assess the effect of a daily dietary supplementation of 2 g calcium over nine months on cell proliferation measured as proliferation index in colonic mucosa. A total of 48 patients were entered into the study of which 30 were fully compliant. After intervention proliferation index % (mean (SEM) in colonic epithelium was decreased in both the calcium (13.5 (1.5) to 11.4 (1.2)) and the placebo group (13.7 (0.9) to 10.8 (1.1)). The difference in the change between the two groups was not significant (p = 0.7). Changes in proliferation index % of crypt compartments were also not significantly different between the two groups. A significantly positive correlation between soluble calcium in faeces and the total proliferation index % in colonic epithelium at baseline and after intervention (r = 0.54, p &lt; 0.01, r = 0.50, p &lt; 0.01 respectively) suggests that an increase of free luminal calcium alone is insufficient for inhibition of cellular proliferation.
Toxic Epidermal Necrolysis Associated With Maternal Use of Heparin
Authors: Alexander Leung
Journal: JAMA (1985)

<h3>To the Editor.—</h3> Heparin-induced cutaneous necrosis unrelated to injection sites<sup>1</sup>as well as following its systemic use<sup>2</sup>have been reported. I describe a case of toxic epidermal necrolysis (TEN) following the maternal use of heparin. <h3>Report of a Case.—</h3> A male neonate weighing 3,350 g was delivered vaginally to a gravida 2, para 0, 34-year-old mother at term. Apgar scores were 7 and 9. During the last two weeks of her pregnancy, the mother had left iliofemoral venous thrombosis and was treated with 1,000 units of heparin per hour intravenously. At birth, the skin of the infant was erythematous. Superficial blisters were noted on the face and trunk. At 48 hours of age, he had peeling of the skin. Nikolsky's sign was positive. Skin culture was negative for bacteria and fungi. Viral study findings were normal. <h3>Comment.—</h3> Two types of TEN are recognized: (1) the staphylococcal "scalded skin"
Werner and Ingbar's The Thyroid: A Fundamental and Clinical Text
Authors: Carl E. Cassidy
Journal: JAMA (1993)

This text is exactly what it purports to be—a fundamental and clinical text. It is divided into nine major parts, which address all aspects of normal thyroid function and disease and laboratory assessment of thyroid function. Each part is divided into sections and chapters. The 95 chapters were written by 116 authors from Australia, Europe, the Middle East, the Far East, and North America.  The volume escapes the varied caliber of individual contributions that plagues some multiauthored books. Instead, the chapters are uniformly excellent, a tribute to the selection of authors combined with superb editing. The contributors are recognized authorities with extensive experience, and the chapters are comprehensive reviews, uncluttered by needless inclusion of all published data. In all but a few instances, the references are extensive but not burdensome. When significant differences of opinion exist, the majority of the authors present fairly the points of view that differ from