tbuckley/cabot-search huggingface dataset:
Abstract 3105: Expression of Wip1, a homeostatic regulator, is competitively controlled by c-Jun and p53 in response to UV irradiation
Authors: Ji-young Song, Ji-young Park, Hyun-mi Kim, Jae-yong Kim, Jene Choi
Journal: Cancer Research (2010)

Abstract Wild-type p53-induced phosphatase (Wip1) is induced by p53 in response to various stresses, resulting in the dephosphorylation of proteins (i.e., p38 MAPK, p53, and uracil DNA glycosylase) involved in DNA repair and cell cycle checkpoint pathways. The p38 MAPK-p53 pathway has been a unique way for Wip1 induction as a stress responder. Here, we show that c-Jun directly binds to and activates the Wip1 promoter in response to UV irradiation. Mutation of the p53 response element (p53RE) or c-Jun consensus sites reduced promoter activities in both non-stressed and stressed A549 cells. Intriguingly, overexpression of p53 significantly decreased Wip1 expression in HCT116 p53+/+ cells but increased Wip1 expression in HCT116 p53−/− cells. Adenovirus-mediated p53 overexpression greatly decreased JNK activity. Up-regulation of Wip1 via the p38 MAPK-p53 and JNK-c-Jun pathways is specific, as demonstrated by our findings that p38 MAPK and JNK inhibitors affected the expression of Wip1 protein, whereas an ERK inhibitor did not. The JNK-c-Jun and p38 MAPK-p53 pathways were activated concomitantly in A549 cells; however, c-Jun activation occurred much more quickly, and to a greater extent, in A549-E6 cells, with delayed but fully induced Wip1 expression. Collectively, these data indicate that Wip1 is activated via both the JNK-c-Jun and p38 MAPK-p53 signaling pathways and that temporal induction of Wip1 largely depends on the balance between c-Jun and p53, because of their competition for JNK binding. Moreover, our results suggest that JNK-c-Jun-mediated Wip1 induction could serve as a major signaling pathway in human tumors, because of frequent p53 mutation. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3105.
Association with the single-nucleotide polymorphism (Glu785Lys) of the granulocyte colony-stimulating factor receptor with myelodysplastic syndromes and acute myeloid leukemia with multlineage dysplasia
Authors: U Platzbecker, U Germing, U Schäkel, T Illmer, S Soucek, M Schaich, G Ehninger, C Thiede
Journal: Leukemia (2006)
397 ROLE OF ACOUSTIC RADIATION FORCE IMPULSE (ARFI) ELASTOGRAPHY FOR THE STAGING OF LIVER FIBROSIS IN CHRONIC HEPATITIS C
Authors: C.G. Fierbinteanu-Braticevici, L. Tribus, A. Petrisor, R. Usvat, C. Busegeanu, I. Stan
Journal: Journal of Hepatology (2010)
Case 76-1961
Authors: Richard C. Cabot, Benjamin Castleman, Betty U. Kibbee
Journal: New England Journal of Medicine (1961)

Presentation of Case A fifty-four-year-old nulliparous woman was admitted to the hospital because of urinary frequency and pain in the abdomen. One year previously a single episode of hematuria occurred. The patient was otherwise asymptomatic until six months before entry, when persistent urinary frequency, urgency and nocturia developed. Two months before admission she began to experience crampy pain in the right lower quadrant of the abdomen before and after urination. The frequency increased, and by the time of admission hourly urination was necessary. There were no chills, fever, loss of weight, nausea, vomiting, anorexia or change in bowel habits. An . . .
A candidate vaccine for hepatitis B containing the complete viral surface protein
Authors: Peter J. Kniskern, Arpi Hagopian, Pamela Burke, Nancy Dunn, Emilio A. Emini, William J. Miller, Shigeko Yamazaki, Ronald W. Ellis
Journal: Hepatology (1988)

The entire surface protein of hepatitis B virus serotype ayw containing the preS (preS1+preS2) and S domains has been expressed in the yeast Saccharomyces cerevisiae. Yeast containing a recombinant plasmid utilizing a constitutive promoter did not express this gene successfully due to the toxicity of the protein. A plasmid using a regulatable promoter directed expression which initiated late in the exponential phase of growth and resulted in the accumulation of high intracellular levels of the complete surface protein. The purified polypeptide aggregates into a form which, although not comprised of typical 20 nm particles, displays antigenic determinants encoded by the preS1, preS2 and S domains. Immunization of rabbits elicited the formation of antibodies directed against all three domains. This candidate vaccine will be useful for studying the contributions to viral immunity of the host response to the preS1 and preS2 domains.
STUDIES ON PRIMARY ATYPICAL PNEUMONIA
Authors: Chien Liu, Monroe D. Eaton, James T. Heyl
Journal: The Journal of Experimental Medicine (1959)

By using the indirect method of fluorescent staining to study the antibody response in patients with primary atypical pneumonai associated with the development of cold agglutinin, it was found that the PAP antibody developed during the 2nd and 3rd week of the illness, and persisted for over a year, and is not related to the cold and streptococcus MG agglutinins. The development of the PAP fluorescent staining antibody paralleled the neutralizing antibody for the PAP virus as tested in cotton rats. The sensitivity of this specific serological test was indicated by the observation that 67 to 92 per cent of the patients in several outbreaks of PAP showed a rise of antibody titer during convalescence. Absorption of the sera with various tissue powders did not affect the PAP antibody detected by this method.
OUTCOMES OF LASER-ASSISTED BALLOON ANGIOPLASTY VERSUS BALLOON ANGIOPLASTY ALONE FOR BELOW KNEE PERIPHERAL ARTERIAL DISEASE
Authors: Chatchawan Piyaskulkaew, Kesav Parvataneni, Hussien Ballout, Tarun Sharma, Mohamed Almahmoud, Lowell Ketron, Susan Szpunar, Thomas LaLonde, Rajendra Mehta, Hiroshi Yamasaki
Journal: Journal of the American College of Cardiology (2014)
Renal Excretion of ^99mTc-Diphosphonate in Osteomalacia
Authors: J. D. MACFARLANE
Journal: Annals of Internal Medicine (1979)

Brief Reports1 March 1979Renal Excretion of 99mTc-Diphosphonate in OsteomalaciaJ. D. MACFARLANE, B.M., M. R. A. KHAIRI, M.D., M. RICCIARDONE, B.S., H. N. WELLMAN, M.D., C. C. JOHNSTON Jr., M.D.J. D. MACFARLANE, B.M.Search for more papers by this author, M. R. A. KHAIRI, M.D.Search for more papers by this author, M. RICCIARDONE, B.S.Search for more papers by this author, H. N. WELLMAN, M.D.Search for more papers by this author, C. C. JOHNSTON Jr., M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-90-3-350 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptInterest in bone scanning in metabolic bone disease has led to the identification of pseudofractures in osteomalacia (1), the assessment of the severity of renal osteodystrophy (2), and the suggestion that calculation of bone to soft-tissue ratios may help distinguish patients with bone disease from normal control persons (3). These techniques are relatively crude and do not necessarily indicate the real skeletal accretion or whole body retention of the radionuclide. During a study of sequential bone to soft-tissue ratios in patients with various bone diseases, we estimated the renal excretion of99mTc-ethane-1-hydroxy-1, 1-diphosphonate (99mTc-EHDP). We report here our findings from...References1. MACFARLANELUTKINBURWOOD JJR: The demonstration by scintigraphy of fractures in osteomalacia. Br J Radiol 50:369-371, 1977 CrossrefMedlineGoogle Scholar2. SYMITTAL WA: Bone scan in chronic dialysis patients with evidence of secondary hyperparathyroidism and renal osteodystrophy. Br J Radiol 48:878-884, 1975 CrossrefMedlineGoogle Scholar3. ROSENTHALLKAYE LM: Technetium-99m-pyrophosphate kinetics and imaging in metabolic bone disease. J Nucl Med 16:33-39, 1975 MedlineGoogle Scholar4. SUBRAMANIANMCAFEEBLAIRKALLFELZTHOMAS GJRFF: Technicum-99m-methylene diphosphonate—a superior agent for skeletal imaging. Comparison with other technetium complexes. J Nucl Med 16:744-755, 1975 MedlineGoogle Scholar5. FOGELMANBESSENTTURNERCITRINBOYLEGREIG IRJDIW: The use of whole body retention of Tc-99m diphosphonate in the diagnosis of metabolic bone disease. J Nucl Med 19:270-275, 1978 MedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Indiana University School of Medicine Indianapolis, Indiana PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByToxische OsteopathienToxische OsteopathienMetabolic bone disease: osteoporosis and osteomalaciaRenal Excretion of Technetium-99m Diphosphonate in OsteomalaciaIGNAC FOGELMAN, RODNEY G. BESSENT 1 March 1979Volume 90, Issue 3Page: 350-351KeywordsBoneExcretionMetabolic disordersRenal diseases Issue Published: 1 March 1979 PDF DownloadLoading ...
Platelet Glycoprotein IIb/IIIa Receptor Antagonists in Cardiovascular Disease
Authors: David A. Vorchheimer
Journal: JAMA (1999)

ContextThrombus formation on disrupted atherosclerotic plaque is the major cause of acute coronary events. Platelet inhibitors are the mainstay of drug therapy to reduce cardiac events in patients with acute coronary syndromes. The platelet glycoprotein (GP) IIb/IIIa receptor is the final common pathway of platelet aggregation.ObjectivesTo review mechanisms of platelet activation and aggregation and the role of the GP IIb/IIIa receptor in the acute coronary syndromes and to summarize completed clinical trials of GP IIb/IIIa receptor antagonists.Data SourcesEnglish-language journal articles, reviews from a MEDLINE search from 1993 through 1998, as well as abstracts and presentations from major national or international cardiology meetings through November 1998.Study Selection/Data ExtractionRandomized, placebo-controlled clinical trials testing intravenous GP IIb/IIIa receptor antagonists and having more than 500 subjects were included. Data quality and validity included publication or presentation venue.Data Synthesis/ConclusionsThe GP IIb/IIIa receptor is the final common pathway of platelet aggregation. Intravenous monoclonal antibody and peptide and nonpeptide antagonists of the GP IIb/IIIa receptor have been tested in randomized, placebo-controlled trials of the acute coronary syndromes and percutaneous coronary interventions. For patients undergoing percutaneous revascularization, these agents have demonstrated efficacy in reducing death, myocardial infarction, or urgent reintervention. Odds ratios of death or myocardial infarction at 30 days range from 0.42 to 0.84 for the drugs in these studies. More modest benefits have been seen in trials of IIb/IIIa receptor antagonists for patients with the acute coronary syndromes, with odds ratios for death or myocardial infarction at 30 days ranging from 0.70 to 0.89. The efficacy of oral agents for chronic GP IIb/IIIa receptor antagonism has not been sufficiently studied.
Effects of sympathetic nerve blockade on vasoconstrictive properties of nitric oxide synthase inhibition in sheep
Authors: M Booke
Journal: Cardiovascular Research (1997)

Objective: Inhibition of nitric oxide synthase causes intense vasoconstriction. This effect is thought to be dependent on sympathetic nerve activity. Thus, we investigated the vasoconstrictive effects of the nitric oxide synthase inhibitor NG-nitro-l-arginine methyl ester (l-NAME) in sheep, in which a reversible sympathetic block was established by thoracic epidural anesthesia. Methods: Sheep (n = 11) were surgically prepared for chronic study. After at least 5 days of recovery, l-NAME was continuously administered and hemodynamics were monitored. This was done in sheep with and without sympathetic blockade in randomized order. Results: The vasoconstrictive effects of l-NAME were similar in sheep with and without sympathetic blockade. Conclusion: The obtained results suggest that the vasoconstrictive properties of nitric oxide synthase inhibitors are independent of sympathetic tone.
Sheffield Medical Society: February 9, 1843
Authors: A. J. Knight
Journal: BMJ (1843)

Churchill to be regarded as the D'Orsay of medical publishers.Nor should the merits of Mr. Bagg be forgotten.He has contributed 246 illustrations of exquisite finish.Well, indeed, would it be, for patients and their surgeons too, if half the jaws and new noses partook of a tithe of the beauty with which Bagg here delineates them.
Coral reef bleaching in the 1980s and possible connections with global warming
Authors: Peter W. Glynn
Journal: Trends in Ecology & Evolution (1991)

Scleractinian corals and their symbiotic dinoflagellate algae build massive, wave-resistant coral reefs that are pre-eminent in shallow tropical seas. This mutualism is especially sensitive to numerous environmental stresses, and has been disrupted frequently during the past decade. Increased seawater temperatures have been proposed as the most likely cause of coral reef bleaching, and it has been suggested that the recent large-scale disturbances are the first biological indication of global warming. This article describes recent bleaching events and their possible link with sea warming and other environmental stresses, and offers some speculation on the fate of coral reefs if the Earth enters a sustained period of warming.
An Outbreak of Hospital-Associated Infections Due to Salmonella Derby
Authors: Eugene Sanders, Francis J. Sweeney, Eli A. Friedman, John R. Boring, Eileen L. Randall, Lewis D. Polk
Journal: JAMA (1963)

An interstate outbreak of hospital-associated infections due to<i>Salmonella derby</i>was first recognized in April, 1963. Between March 1 and Aug 12, 1963, 822 isolations of<i>Sal derby</i>were made from patients, staff, or employees of 53 hospitals in 13 states. Epidemiological investigations indicated that the organism had been introduced into the hospitals by a common source involved in interstate movement. Raw or undercooked eggs were implicated as the common source vehicle of infection. Extensive secondary spread of infection, presumably person to person, has more recently become a major problem in several institutions. Studies to define the mode of spread of secondary infections are currently in progress.
IMPLICATIONS OF CORONARY ARTERY CALCIUM SCORE ON SAMPLE SIZE CALCULATIONS IN CARDIOVASCULAR PRIMARY PREVENTION TRIALS: THE MULTI-ETHNIC STUDY OF ATHEROSCLEROSIS (MESA)
Authors: Marcio Sommer Bittencourt, Ron Blankstein, Jaideep Patel, Michael Blaha, Edward Hulten, Roger Blumenthal, Leslee Shaw, Songtao Liu, John Eng, Arthur Agatston, Matthew Budoff, Khurram Nasir
Journal: Journal of the American College of Cardiology (2014)
Rho is Required for the Initiation of Calcium Signaling and Phagocytosis by Fcγ Receptors in Macrophages
Authors: David J. Hackam, Ori D. Rotstein, Alan Schreiber, Wei-jian Zhang, Sergio Grinstein
Journal: The Journal of Experimental Medicine (1997)

Phagocytosis of bacteria by macrophages and neutrophils is an essential component of host defense against infection. The mechanism whereby the interaction of opsonized particles with Fcγ receptors triggers the engulfment of opsonized particles remains incompletely understood, although activation of tyrosine kinases has been recognized as an early step. Recent studies in other systems have demonstrated that tyrosine kinases can in turn signal the activation of small GTPases of the ras superfamily. We therefore investigated the possible role of Rho in Fc receptor–mediated phagocytosis. To this end we microinjected J774 macrophages with C3 exotoxin from Clostridium botulinum, which ADP-ribosylates and inactivates Rho. C3 exotoxin induced the retraction of filopodia, the disappearance of focal complexes, and a global decrease in the F-actin content of J774 cells. In addition, these cells exhibited increased spreading and the formation of vacuolar structures. Importantly, inactivation of Rho resulted in the complete abrogation of phagocytosis. Inhibition of Fcγ receptor–mediated phagocytosis by C3 exotoxin was confirmed in COS cells, which become phagocytic upon transfection of the FcγRIIA receptor. Rho was found to be essential for the accumulation of phosphotyrosine and of F-actin around phagocytic cups and for Fcγ receptor–mediated Ca2+ signaling. The clustering of receptors in response to opsonin, an essential step in Fcγ-induced signaling, was the earliest event shown to be inhibited by C3 exotoxin. The effect of the toxin was specific, since clustering and internalization of transferrin receptors were unaffected by microinjection of C3. These data identify a role for small GTPases in Fcγ receptor–mediated phagocytosis by leukocytes.
Thymus-homing peripheral dendritic cells constitute two of the three major subsets of dendritic cells in the steady-state thymus
Authors: JiChu Li, JooHung Park, Deborah Foss, Irving Goldschneider
Journal: The Journal of Experimental Medicine (2009)

Many dendritic cells (DCs) in the normal mouse thymus are generated intrathymically from common T cell/DC progenitors. However, our previous work suggested that at least 50% of thymic DCs originate independently of these progenitors. We now formally demonstrate by parabiotic, adoptive transfer, and developmental studies that two of the three major subsets of thymic DCs originate extrathymically and continually migrate to the thymus, where they occupy a finite number of microenvironmental niches. The thymus-homing DCs consisted of immature plasmacytoid DCs (pDCs) and the signal regulatory protein α–positive (Sirpα+) CD11b+ CD8α− subset of conventional DCs (cDCs), both of which could take up and transport circulating antigen to the thymus. The cDCs of intrathymic origin were mostly Sirpα− CD11b− CD8αhi cells. Upon arrival in the thymus, the migrant pDCs enlarged and up-regulated CD11c, major histocompatibility complex II (MHC II), and CD8α, but maintained their plasmacytoid morphology. In contrast, the migrant cDCs proliferated extensively, up-regulated CD11c, MHC II, and CD86, and expressed dendritic processes. The possible functional implications of these findings are discussed.
MEDIAN BAR EXCISOR
Authors: W. F. BRAASCH
Journal: JAMA (1918)

It is generally recognized that urinary obstruction at the vesical orifice may be caused by various conditions other than prostatic hypertrophy. Such conditions may be divided into three groups, namely, contracture of the bladder neck, glandular and inflammatory tissue formation, and congenital obstructions. Methods have been devised for removing the obstruction caused by contraction at the vesical orifice, such as the Chetwood and modified Bottini operations, and the Goldschmit technic. However, n o method short of resection had been definitely suggested for the removal of obstructing glandular and inflammatory tissue until that described by Young in 1912. He demonstrated that such obstructions could be removed by endoscopic methods under local anesthesia, and without incapacitating the patient for more than a few days. The instrument he used was called a median bar excisor, and while the instrument was indeed a very serviceable one, the operator was handicapped by the fact that
Factors Associated With Use of Cardiopulmonary Resuscitation in Seriously Ill Hospitalized Adults
Authors: Sarah J. Goodlin
Journal: JAMA (1999)

ContextThe epidemiology of do-not-resuscitate (DNR) orders for hospitalized patients has been reported, but little is known about factors associated with the use of cardiopulmonary resuscitation (CPR).Objective To identify factors associated with an attempt at CPR for patients who experienced cardiopulmonary arrest.Design Secondary analysis of data collected in 2 prospective cohort studies: the Study to Understand Prognoses and Preferences for Outcomes and Risks of Treatments (SUPPORT, 1989-1994) and the Hospitalized Elderly Longitudinal Project (HELP, 1994). Setting Five teaching hospitals across the United States.Participants A total of 2505 seriously ill hospitalized patients and nonelectively admitted persons aged 80 years or older who experienced cardiopulmonary arrest.Main Outcome Measures Medical records data on CPR efforts, DNR orders, disease severity, age, race, sex, length of stay, and survival; functional status and preferences concerning CPR obtained by interviews with patients or surrogates; and 2-month survival estimates provided by physicians. ResultsFive hundred fourteen study subjects (21%) received CPR during their index hospitalization.Among them, 327 (63.6%) had CPR within 2 days of death and 93 (18.1%) had resuscitation and survived their index hospitalization.Use of CPR was more likely in men (odds ratio [OR], 1.39; 95% confidence interval [CI], 1.12-1.73),younger patients (OR per 10-year increase, 0.90; 95% CI, 0.84-0.96),African Americans (OR, 1.76; 95% CI, 1.33-2.34),patients whose reported preferences were for CPR (OR, 2.60; 95% CI, 1.91-3.55),who reported better quality of life (OR, 1.49; 95% CI, 1.10-2.03),or who had higher physician estimates for 2-month survival (OR per 10% increase, 1.14; 95% CI, 1.09-1.19).Rates varied significantly with geographic location and diagnosis; the adjusted OR for patients with congestive heart failure was 3.31 (95% CI, 2.12-5.15)compared with patients with acute respiratory failure or multiple organ system failure. ConclusionsOur data suggest that a resuscitation attempt is more likely when preferred by patients and when death is least expected.Further study is required to understand variation in use of CPR among sites and for patients with different diagnoses, race, sex, or age.
An Outbreak of Fatal Fluoride Intoxication in a Long-Term Hemodialysis Unit
Authors: Paul M. Arnow
Journal: Annals of Internal Medicine (1994)

To determine the cause of an outbreak of acute illness and death in a long-term hemodialysis unit.A retrospective cohort and case-control study of patients receiving hemodialysis and a laboratory study of a model deionization system to purify water for hemodialysis.An outpatient hemodialysis unit of a university hospital.12 patients who became severely ill after hemodialysis treatment and 20 patients who did not become ill after receiving hemodialysis treatment in the same unit.Medical and dialysis unit records were reviewed to identify and characterize cases. Fluids for dialysis were tested for toxic substances, and fluoride was measured in patients' serum. Resistivity and fluoride were measured in effluent from a model deionization system operated in the same way as the system associated with illness.During five consecutive hemodialysis shifts, 12 of 15 patients receiving dialysis treatment in one room became acutely ill, with severe pruritus, multiple nonspecific symptoms, and/or fatal ventricular fibrillation (3 patients). None of 17 patients treated in the adjacent room became ill (P < 0.0001). Death was associated with longer hemodialysis time and increased age compared with other patients who became ill. Serum concentrations of fluoride in the sick patients were markedly increased to as high as 716 mumol/L, and the source of fluoride was the temporary deionization system used to purify water for hemodialysis only in the affected room. Operation of a model deionization system showed how fluoride was adsorbed and then displaced in a massive efflux.Because deionization systems are used widely in hemodialysis and can cause fatal fluoride intoxication, careful design and monitoring are essential.
Serum enzyme elevation in glutethimide intoxication.
Authors: L W Henderson, M Metz, J H Wilkinson
Journal: BMJ (1970)

While the diagnostic usefulness of serum enzyme tests has un- doubtedly been established the emphasis on their diagnostic specificities is sometimes misplaced.It is often more appropri- ate to interpret serum enzyme activities in the light of prob- able tissue injury rather than of specific diagnoses.This is illustrated by serial studies recently performed on two patients with acute glutethimide intoxication.