tbuckley/cabot-search huggingface dataset:
Abstract 503: Investigating the role of Tks5 and invadopodia in prostate tumor progression
Authors: Karen L. Burger, Brian S. Learman, Amy K. Boucherle, Darren F. Seals
Journal: Cancer Research (2012)

Abstract Much of the morbidity and mortality in prostate cancer patients occurs strictly in response to metastatic disease. While ongoing efforts are examining the therapeutic value of Src kinase inhibitors, the mechanism by which Src activates invasive cell behavior within the context of this disease are not well-understood. Invadopodia are cytoskeletal structures formed by cancer cells that enable binding to, proteolytic degradation of, and invasion through the extracellular matrix. We and others have previously demonstrated that the Src tyrosine kinase substrate and adaptor protein Tks5 is important for invadopodia formation and function. Here we demonstrate that Src is activated and Tks5 up-regulated in prostate tumor specimens and invasive cell lines. Remarkably, overexpression of Tks5 alone is sufficient to form invadopodia structures and enhance invadopodia-associated gelatin degradation and invasion in the LNCaP cell line. We further show that this increase in invasive behavior likely depends on Src kinase activity and Tks5 phosphorylation. Our results predict a role for Src-Tks5 signaling in prostate tumor progression. This hypothesis will be tested in future studies using (i) mouse xenografts to monitor tumor development from Tks5-modified prostate cancer cell lines, and (ii) human prostate tumor tissue sections to measure Tks5 staining relative to tumor grade. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 503. doi:1538-7445.AM2012-503
Cutaneous Venoconstrictor Response to Local Cooling in the Dog
Authors: MICHAEL M. WEBB-PEPLOE
Journal: Circulation Research (1969)

Studies were performed in dogs to determine if inhibition of neuronal reuptake of norepinephrine was the cause of the enhanced response to sympathetic nerve impulses seen in the cutaneous veins when the temperature of the blood perfusing them was decreased. Changes in driving pressure of the saphenous vein perfused with blood at constant flow were used to measure venomotor responses. The increases in venoconstrictor response to norepinephrine infusion caused by cooling the perfusate were similar in both hindlegs, one subjected to acute and the other to chronic sympathectomy. Blocking the reentry of norepinephrine into the sympathetic nerve endings by treating the vein with cocaine did not affect the augmentation by local cooling of the constrictor response to norepinephrine or electric stimulation of the lumbar sympathetic chain. The venoconstrictor responses to norepinephrine, epinephrine, 5-hydroxytryptamine, and molar potassium chloride were all augmented by local cooling. The results suggest that the sensitizing effect of cold on the smooth muscle of the veins cannot be explained by inhibition of norepinephrine re-uptake by the nerve terminals.
Epstein-Barr Virus in Pediatric Multiple Sclerosis
Authors: Suad Alotaibi
Journal: JAMA (2004)

Infection with common viruses, particularly Epstein-Barr virus (EBV), has been postulated to contribute to the pathobiology of multiple sclerosis (MS). Detailed virological studies in pediatric MS have not been previously reported.To evaluate whether children with MS are more likely to be seropositive for EBV or other common viruses than their healthy age-matched peers.Case-control study of viral samples collected from March 1994 to February 2003 from 30 pediatric MS patients, 90 emergency department controls matched 3:1 with the MS patients by year of birth, and 53 healthy control children.Archived serum samples were analyzed for the presence of IgG antibodies directed against EBV viral capsid antigens, nuclear antigens, and early antigens, cytomegalovirus, parvovirus B19, herpes simplex virus, and varicella zoster.Serological evidence for remote EBV infection was present in 83% of pediatric MS patients compared with 42% of emergency department and healthy controls (P<.001). Five pediatric MS patients were negative for all 3 EBV antigens. Pediatric MS patients were less likely than controls to have been exposed to herpes simplex virus (P =.003), while seropositivity for cytomegalovirus, parvovirus B19, and varicella zoster did not differ between MS patients and controls.These results suggest an association between EBV infection and pediatric MS.
Granuloma Inguinale with Hematogenous Dissemination to the Spine
Authors: Bernard M. Kalstone
Journal: JAMA (1961)

GRANULOMA INGUINALE with metastases to bone is a comparatively rare occurrence. In the United States, primary granuloma inguinale has apparently been on the increase in the last few years as have the other venereal diseases. The placing of granuloma inguinale in the venereal category is actually an arbitrary one, as this condition has never been absolutely proved to be of venereal origin. It is the proclivity of this disease for the genital region that qualifies it to be listed as a venereal infection. In this paper granuloma inguinale and its complications will be reviewed, and a case of this disease with unusual sequelae will be presented.  Granuloma inguinale is a chronic granulomatous process that involves the skin and subcutaneous tissue of the genital region. The etiologic agent is the Donovan body. This is an intracellular inclusion body first described by Donovan in 1904. The true nature of the Donovan
Migration of Vδ1 and Vδ2 T cells in response to CXCR3 and CXCR4 ligands in healthy donors and HIV-1–infected patients: competition by HIV-1 Tat
Authors: Alessandro Poggi, Roberta Carosio, Daniela Fenoglio, Sabrina Brenci, Giuseppe Murdaca, Maurizio Setti, Francesco Indiveri, Silvia Scabini, Elisabetta Ferrero, Maria Raffaella Zocchi
Journal: Blood (2003)
Does cutting of mugwort stands affect airborne pollen concentrations?
Authors: A. Rantio‐Lehtimäki, M. L. Helander, K. Karhu
Journal: Allergy (1992)

Pollen of mugwort (Artemisia vulgaris L.) is the most important allergenic pollen in urban areas of south and central Finland in late summer. The purpose of this study was to investigate, experimentally, whether the cutting of mugwort stands affects its airborne pollen concentrations. Experimental plots were either cut (4 plots) or uncut (4 plots) in 2 previous seasons: 4 of them were small (less than 0.5 hectare) and 4 large (greater than 5 hectares). Finally, the plots were divided randomly into 2 groups according to a third variable, cutting in the study season, 1989. Samples were taken on 2 rainless mornings at the peak mugwort flowering time. Two rotorod type samplers were used at heights of 1 and 2 m from ground level, simulating the inhalation heights of children and adults, respectively. The results indicate that cutting mugwort stands significantly reduces airborne pollen concentrations, but the treated areas have to be large, since in the town area there are plenty of mugwort pollen sources. The pollen concentrations at the 2 heights tested did not differ significantly.
A Controlled Study of Adenoviral-Vector–Mediated Gene Transfer in the Nasal Epithelium of Patients with Cystic Fibrosis
Authors: Michael R. Knowles, Kathy W. Hohneker, Zhaoqing Zhou, John C. Olsen, Terry L. Noah, Ping-Chuan Hu, Margaret W. Leigh, John F. Engelhardt, Lloyd J. Edwards, Kim R. Jones, Mariann Grossman, James M. Wilson, Larry G. Johnson, Richard C. Boucher
Journal: New England Journal of Medicine (1995)

Cystic fibrosis is a monogenic disease that deranges multiple systems of ion transport in the airways, culminating in chronic infection and destruction of the lung. The introduction of a normal copy of the cystic fibrosis transmembrane conductance regulator (CFTR) gene into the airway epithelium through gene transfer is an attractive approach to correcting the underlying defects in patients with cystic fibrosis. We tested the feasibility of gene therapy using adenoviral vectors in the nasal epithelium of such patients.
Doctors who own scanning equipment are more likely to order scans, study shows
Authors: B. Roehr
Journal: BMJ (2011)

US doctors with a financial interest in magnetic resonance imaging (MRI) equipment, either owning or leasing it, are more likely to order scans for lower back pain than doctors who have no such stake. And those who are orthopaedic specialists are more likely to go on to order surgery, finds a new study published in Health Services Research (doi:10.1111/j.1475-6773.2011.01265.x).

The study was based on regression modelling of data from Medicare, the national health insurance programme for people 65 or older, for the period 1998 to 2005. Around 2.8 million visits to doctors over that period were for non-specific lower back pain, and the study sampled …
248. A Mouse Model of Type VII Glycogenosis to Assay Gene Therapy Approaches
Authors: Fatima Bosch, Miquel Garcia, Anna Pujol, Albert Ruzo, Anna Arbos, Juan E. Feliu
Journal: Molecular Therapy (2005)

Top of pageAbstract
Type VII glycogenosis, or Tarui's disease, is a muscular disorder characterized by the appearance of muscular fatigue, hemolysis and myoglobinuria after exercise. It is associated with an abnormal accumulation of muscle glycogen, due to an increase in its synthesis and a blockade of its mobilization. The underlying defect is a deficiency in muscle 6-phosphofructo-1-kinase isoenzyme (PFK-1M). In order to obtain a murine model of Tarui's disease, heterozygous and homozygous PFK-1M knock-out mice were generated by standard gene-targeting methods in mouse ES cells. Heterozygous mice showed a 50% decrease in muscle PFK-1M mRNA levels, which led to a 50% decrease in muscle enzyme activity. This decrease did not cause alterations in muscle glycogen levels. Neither expression of the gene nor activity of the enzyme was found in skeletal muscle extracts from homozygous mice. These animals showed a three-fold increase in muscle glycogen levels and morphological abnormalities in muscle fibers such as necrotic and regenerating fibers or altered mitochondrias. Histopathological analysis of other tissues revealed glycogen accumulations in cardiac muscle and diaphragm. Moreover, due to the loss of erythrocyte PFK-1M activity, these animals showed higher levels of circulating reticulocytes. In addition, the spleen of PFK-1M deficient animals was 5 times bigger than those in control animals, due to hemolysis and compensatory extramedullary erythropoiesis. Furthermore, these animals were intolerant to exercise as described in human patients.

The results indicate that mice lacking PFK-1M show pathological alterations similar to those observed in patients with type VII glycogenosis. Thus, these animals are the first murine model of type VII glycogenosis in which the disease can be studied and gene therapy approaches can be assayed and developed.
Selective targeting of the LIGHT-HVEM costimulatory system for the treatment of graft-versus-host disease
Authors: Yanhui Xu, Andrew S. Flies, Dallas B. Flies, Gefeng Zhu, Sudarshan Anand, Sarah J. Flies, Haiying Xu, Robert A. Anders, Wayne W. Hancock, Lieping Chen, Koji Tamada
Journal: Blood (2006)
Recombinant Newcastle Disease Virus as a Vaccine Vector for Cancer Therapy
Authors: Adam Vigil, Osvaldo Martinez, Mark A Chua, Adolfo García-Sastre
Journal: Molecular Therapy (2008)

Naturally occurring strains of Newcastle disease virus (NDV) are currently being investigated in multiple clinical trials for oncolytic cancer therapy in the United States and abroad. We have previously reported, for the first time, the development of recombinant NDVs designed for enhanced cancer therapeutic efficacy. Specifically, we have shown that NDV engineered to express interleukin-2 (IL-2) generates a robust therapeutic response associated with increased tumor-specific T-cell infiltration after intratumoral administration in mice. We have now demonstrated that this therapeutic response is dependent on T cells and we have investigated the potential to focus the NDV-induced immune response toward a tumor-associated antigen (TAA) to enhance the inherent therapeutic efficacy of NDV further. We found that intratumoral treatments of tumor-bearing mice with recombinant NDV expressing a model TAA elicited an enhanced tumor-specific response, resulting in a significant increase in the number of complete tumor regressions compared with control NDV. Additionally, coadministration of NDV expressing a model TAA with NDV expressing IL-2 enhanced the TAA-directed response and led to more complete tumor regressions. Our results show that TAA-directed immunotherapy by oncolytic recombinant NDV alone or in combination with IL-2 results in an enhanced therapeutic efficacy and warrant consideration in the development of cancer therapies based on the use of oncolytic NDV.
Time domain and flow indices of bronchial hyperresponsiveness: association with asthma symptoms, atopy and smoking
Authors: M.R. Miller, T. Sigsgaard, O. Omland, O.F. Pedersen
Journal: European Respiratory Journal (2002)

Conventional measures of bronchial hyperresponsiveness (BHR) are only weakly associated with respiratory symptoms in epidemiological studies. Partial and maximal forced expiratory manoeuvres were recorded during histamine challenge testing in 1,959 young male farmers. Analysis was performed to test whether novel measures of BHR, using alternative flow and time domain indices, are more closely associated with asthma symptoms, smoking status and atopy than forced expiratory volume in one second (FEV 1 ) and conventional measures of BHR. The first moments to 75% and 90% of the forced vital capacity (FVC) were calculated from full (F) and partial (P) forced expiratory manoeuvres ( i.e. α 1 75%F, α 1 75%P), together with the instantaneous flows when 40% and 30% of the FVC remained to be expired (MEF 40 and MEF 30 ). BHR was measured by the provocative dose causing a 20% change (PD 20 ) in the FEV 1 and α 1 75%, and also by the method of log dose slopes (LDS). Asthma was diagnosed from symptoms associated with asthma in 158 (8.1%) of the subjects. PD 20 FEV 1 could only be recorded in 190 subjects (9.7%), of whom only 48 had asthma, whereas LDSFEV 1 was recorded in 1,725 (88%) subjects. From the prechallenge data, α 1 75%, expressed as standardised residuals, showed the largest difference between smokers with and without asthma symptoms, and no indices showed significant differences between nonsmokers with and without asthma symptoms. From BHR data in both smokers and nonsmokers, LDSFEV 1 showed one of the largest differences between those with and without asthma symptoms. With smoking status and atopy accounted for, the greatest partial correlation with asthma diagnosis was found for LDSMEF 40 P, and then for LDSFEV 1 , but LDSMEF 40 P was measurable in only just over one-half of the subjects. The authors conclude that time-domain indices are promising measures for longitudinal epidemiological studies concerning the relationship between bronchial hyperresonsiveness and environmental exposures. However, indices from the partial flow-volume loop suffer from censored data.
Infant-feeding Practices
Authors: E. Stewart
Journal: BMJ (1973)

despite vaccination and a good "take," can develop modified smallpox.With regard to the question of severe chickenpox versus smallpox, I think it is sensible and wise to err on the wrong side and regard all severe cases of chickenpox as potential cases of smallpox until proved otherwise.In such situations, in my ex- perience, mapping of the lesions on a ohart and comparing it with Langer's lines2 as shown in the accompanying figure has been found very useful, as the distribution of lesions in chickenpox follows the Langer's lines very closely, whereas those of smallpox do not.Lastly, with regard to laboratory diagnosis I think agar-gel diffusion based on antigen and antibody reaction, a test devised by Dumbell and Nizamuddin,3 has proved outstandingly successful, giving reliable, rapid results in about six hours.-Iam, etc.,
Abstract 3661: Notch2 and Six1 are upregulated during progression of early-stage lung adenocarcinoma and define its unfavorable subset at advanced stages
Authors: Takahiro Mimae, Morihito Okada, Man Hagiyama, Yoshihiro Miyata, Yasuhiro Tsutani, Takao Inoue, Kenjiro Aogi, Yoshinori Murakami, Akihiko Ito
Journal: Cancer Research (2012)

Abstract Lung adenocarcinoma (LA) often manifests as tumors with mainly lepidic growth (LG) pattern in which cancer cells replace alveolar cells, and is diagnosed, depending on the size of invasive foci, as in situ, minimally invasive, or invasive LA. This suggests that a subset of LAs undergoes malignant progression in this order. The present study examines how transcriptional aberrations during the early-stage of LA cells define the clinical phenotypes of advanced tumors. First, we comprehensively searched differentially expressed genes between preinvasive and invasive cancer cells in a single minimally invasive LA using laser capture microdissection and DNA microarrays, and successfully identified two transcription factors Notch2 and Six1 to be up-regulated in the invasive cancer cells from all of five minimally invasive LA tumors. These two molecules appeared to coordinately contribute to LA progression by inducing epithelial-mesenchymal transition and nuclear atypia, because exogenous Notch2 in NCI-H441 lung epithelial cells caused nuclei to enlarge and transactivated Six1, vimentin, and the TGF-β signal transducers Smad3 and Smad4. We then immunohistochemically stained 64 advanced LA tumors, and found that a third of them were double positive for Notch2 and Six1 in the invasive, but not in the LG component. The invasive potential was higher and 2-year disease-free survival rates were lower in tumors that were double-positive, than double negative in both components. These results suggested that paired up-regulation of Notch2 and Six1 during early LA progression persists in a considerable subset of advanced LAs and confers a more malignant phenotype on the subset. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3661. doi:1538-7445.AM2012-3661
John Leonard Weeks
Authors: Björn Weeks
Journal: BMJ (2007)

Professor of surgery who developed evidence based surgery and medical ethicsAlan Johnson rose rapidly through the ranks of academic surgery to become reader in surgery at Charing Cross Hospital in 1971, a post he held until moving to the chair in surgery in Sheffield in 1979.He combined excellence in surgical skills, sharp scientific aptitude, a rigorous approach to developing evidence based surgery, and a strong interest in medical ethics.He translated these interests into a body of published work which made an important contribution to the disciplines of gastroduodenal motility, hepatobiliary and upper gastrointestinal surgery, randomised surgical trials, and medical ethics.In total, he edited or authored 10 textbooks, over 35 book chapters, and almost 200 original publications.Two of his books were published after his retirement, including a text on surgical palliative care, and his last book, entitled Making Sense of Medical Ethics and written jointly with his son Paul, was completed a month before his death.He published prospective randomised trials evaluating the role of lithotripsy in the treatment of gallstones and a trial comparing laparoscopic with mini-and evaluated new techniques throughout his surgical career and latterly had been a pioneer in the surgical treatment of obesity.
THE RATE OF INTRAPULMONARY BLOOD GAS EXCHANGE IN LIVING ANIMALS*
Authors: Jorge Soni, Khalil A. Feisal, Arthur B. DuBois
Journal: Journal of Clinical Investigation (1963)

The respiratory function of the blood depends on a series of reversible reactions of oxygen and carbon dioxide with blood. The optimal performance of this function requires that the rate of these reactions be such as to permit them to approach completion within the time the blood spends in the vessels of gas exchange. In the case of carbon dioxide, experiments by Roughton (1) and more recently by Forster (2), using different methods, have suggested that the reactions involved may be slow enough to limit the excretion of CO2 in the lung. Most of the work in this field has been performed in vitro, and because of the difficulty in carrying over the information obtained to living systems, we have devised a technique by which some aspects of the reactions leading to CO2 liberation in the lung can be studied in living animals.
Doxorubicin Hypersensitivity and Clindamycin
Authors: Francis P. Arena, Susan Sherlock
Journal: Annals of Internal Medicine (1990)

Letters and Corrections15 January 1990Doxorubicin Hypersensitivity and ClindamycinFrancis P. Arena, MD, Susan Sherlock, MDFrancis P. Arena, MDSearch for more papers by this author, Susan Sherlock, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-112-2-150_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the Editor: Doxorubicin hydrochloride (Adriamycin, Adria Laboratories, Dublin, Ohio) and daunomycin anthracycline antibiotics are derived from Streptomyces peucetius var. caesius. There have been only a few reports (1, 2) of urticarial reactions to intravenous administration of Adriamycin or daunomycin and even fewer of more systemic allergic reactions to Adriamycin administration (3). We report two cases in which patients had a significant allergic reaction to Adriamycin or daunomycin. The patients had either previous exposure or a history of allergy to clindamycin.A 5 8-year-old white woman diagnosed with stage III diffuse histiocytic lymphoma received gentamicin, clindamycin, and cefazolin after laparotomy....References1. SouhamiFeld LR. Urticaria following intravenous doxorubicin administration. JAMA. 1978;240:1624-6. CrossrefMedlineGoogle Scholar2. Vogelzang N. "Adriamycin Flare". A Skin Reaction Resembling Extravasation. Cancer Treatment Reports. 1979;2067-9. MedlineGoogle Scholar3. ArnoldStafford DC. Systematic allergic reactions to Adriamycin. Cancer Treatment Reports. 1979;63:150-1. MedlineGoogle Scholar4. Upjohn Pharmaceutical Publications. No. 8831-07. Kalamazoo, Michigan: Upjohn; February 1983:9. Google Scholar5. Package Insertion for Adriamycin. Dublin, Ohio: Adria Laboratories. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 15 January 1990Volume 112, Issue 2Page: 150-150KeywordsAllergy and immunologyAntibioticsClindamycinDiffuse large B-cell lymphomaHypersensitivityLaparotomy Issue Published: 15 January 1990 PDF downloadLoading ...