tbuckley/cabot-search huggingface dataset:
Hands-on Workshops as An Effective Means of Learning Advanced Technologies Including Genomics, Proteomics and Bioinformatics
Authors: Nichole Reisdorph, Robert Stearman, Katerina Kechris, Tzu Lip Phang, Richard Reisdorph, Jessica Prenni, David J. Erle, Christopher Coldren, Kevin Schey, Alexey Nesvizhskii, Mark Geraci
Journal: Genomics Proteomics & Bioinformatics (2013)

Abstract Genomics and proteomics have emerged as key technologies in biomedical research, resulting in a surge of interest in training by investigators keen to incorporate these technologies into their research. At least two types of training can be envisioned in order to produce meaningful results, quality publications and successful grant applications: (1) immediate short-term training workshops and (2) long-term graduate education or visiting scientist programs. We aimed to fill the former need by providing a comprehensive hands-on training course in genomics, proteomics and informatics in a coherent, experimentally-based framework. This was accomplished through a National Heart, Lung, and Blood Institute (NHLBI)-sponsored 10-day Genomics and Proteomics Hands-on Workshop held at National Jewish Health (NJH) and the University of Colorado School of Medicine (UCD). The course content included comprehensive lectures and laboratories in mass spectrometry and genomics technologies, extensive hands-on experience with instrumentation and software, video demonstrations, optional workshops, online sessions, invited keynote speakers, and local and national guest faculty. Here we describe the detailed curriculum and present the results of short- and long-term evaluations from course attendees. Our educational program consistently received positive reviews from participants and had a substantial impact on grant writing and review, manuscript submissions and publications.
Addiction to temazepam.
Authors: L Ratna
Journal: BMJ (1981)

Case reportA 66-year-old man presented in April 1979 with a flu-like illness and pleuritic chest pain.He was referred for investigation four months later because of persisting fatigue and lassitude.He had developed pain and stiffness of the neck and shoulders but no other symptoms.Examination showed a blood pressure of 220/110 mm Hg, an exudative retinopathy, and albuminuria.The liver was moderately enlarged.Haemo- globin concentration and white cell and platelet counts were normal.Erythrocyte sedimentation rate was 106 mm in first hour.Blood urea con- centration was 6-8 mmol/l (41 mg/100 ml), plasma sodium 141 mmol (mEq)/l, potassium 4-1 mmol(mEq)/l, and creatinine 73 Htmol/l (0-82 mg/ 100 ml).Creatinine clearance was 68 ml/min and urinary protein 6 g/24 h, and red and white cell casts were seen in the urinary deposit.An intravenous pyclogram was normal.A renal biopsy specimen showed focal glomerulonephritis (figure).ThereSingle glomerulus showing focal glomerulonephritis with capsular epithelial proliferation.(Haematoxylin and eosin x 480 (original magnification).)was focal lobular obliteration and fibrinoid deposition, with focal thickening of the peripheral capillary loops, occasional epithelial crescent formation, and a diffuse increase in mesangium.Immunofluorescence staining of fresh material showed heavy granular deposition of IgG, IgA, IgM, C3, and Clq in both the capillary loops and mesangium.Ultrastructural examination of glutaraldehyde-fixed material showed electron-dense immune deposits in the mesangium and beneath the epithelial cells in the capillary loops.There were no subendothelial deposits.The morphological features, except for the absence of subendothelial deposits, were suggestive of systemic lupus erythematosus.Antinuclear factor was subsequently detected in the serum at a dilution of 1 in 128, but DNA binding was only 5 % (thus he had four of the American Rheumatism Association criteria for systemic lupus erythema- tosus).Several tests of liver function were abnormal.Serum alkaline phosphatase activity was 560 U/l (normal 21-94 Ul1), aspartate transferase activity 52 U/l (normal 8-40 U/l), and albumin concentration 31 g/l (normal 35-53 gl1).A liver biopsy specimen showed moderate infiltration of mononuclear cells in the portal tracts with considerable fibrosis and focal mild piecemeal necrosis.There were no granulomas, but the bile duct showed considerable periductal inflammation and epithelial damage.The features were consistent with primary biliary cirrhosis.Tests for antimitochondrial antibody were positive and serum IgM was 360 IU/ml (normal 69-322 IU/ml).
BRIEF PSYCHOTHERAPEUTIC INTERVIEWS IN THE TREATMENT OF EPILEPSY
Authors: OSKAR DIETHELM
Journal: American Journal of Psychiatry (1947)

Brief psychotherapeutic interviews try to utilize the dynamic knowledge of the individual patient's past life and to apply it to a satisfactory adjustment to the present. His hopes and plans for the future are constantly taken into consideration. Difficulties which precipitate maladjustments are reviewed from the same point of view. The treatment considers impaired functions, such as disorders of attention, concentration and thinking, in the technique of the interview. Recurrent resentment and anxiety may be relieved through a necessarily very limited analysis and their intensity reduced. The persistence of these destructive emotions as well as the presence of other psychopathological symptoms may limit or prevent an analysis which would give the patient an understanding of the dynamic factors. In order to proceed one must correct the factors which lead to therapeutic obstructions or attempt the dynamic analysis when the patient becomes ready for it. This type of psychotherapy must use a plan of treatment which covers a long period of time and which is reconsidered and modified whenever psychopathological changes or psychodynamic, somatic or environmental factors make it necessary. The results of the patient's adjustment of, and to, his life problems are encouraging enough to justify his physician's active therapy and guidance during the long period of the epileptic disorder.
Time to Hang Up the White Coat? Epidemiologists Suggest Ways to Prevent Clothing From Spreading Infection
Authors: Bridget M. Kuehn
Journal: JAMA (2014)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
THE SPLANCHNIC REMOVAL IN RABBITS DURING FATAL BACTERIEMIAS OF THE CIRCULATING ORGANISMS AND OF SUPERIMPOSED NON-PATHOGENIC BACTERIA
Authors: Samuel P. Martin, Grace P. Kerby
Journal: The Journal of Experimental Medicine (1950)

1. By a method of hepatic venous catheterization previously described, comparative data have been obtained concerning the removal of pneumococci from the splanchnic circulating blood of the intact rabbit in bacteriemias secondary to a dermal infection and in bacteriemias induced by a continuous infusion of organisms into the blood stream. The average splanchnic removal in secondary pneumococcal bacteriemias was 8 +/- 14 per cent (S.D.), in induced bacteriemias 9 +/- 15 per cent (S.D.). 2. Similar data were obtained in induced M. aureus bacteriemias in normal rabbits and in induced M. aureus bacteriemias superimposed upon pneumococcal bacteriemias secondary to dermal infection. The average splanchnic removal of M. aureus in normal rabbits was 62 +/- 20 per cent (S.D.). The splanchnic removal of M. aureus in rabbits with a simultaneous pneumococcal bacteriemia of infection was of the same order of magnitude as in normal rabbits. 3. The efficiency of the mechanism of splanchnic removal for a given organism is an important factor in the establishment and maintenance of a bacteriemia. Present evidence indicates that the maintenance of a bacteriemia requires a constant introduction into the blood stream of organisms from some source. 4. Pneumococci do not acquire a resistance to removal from the splanchnic circulating blood during the course of fatal infection in rabbits. 5. No final exhaustion of the mechanism of splanchnic removal can be demonstrated in fatal pneumococcal bacteriemias in rabbits.
Influence of loading conditions and contractile state on pulmonary venous flow. Validation of Doppler velocimetry.
Authors: B D Hoit, Y Shao, M Gabel, R A Walsh
Journal: Circulation (1992)

BACKGROUND Although recent studies suggest that pulmonary venous flow velocities may be used to evaluate left ventricular diastolic function, the influence of loading conditions and contractile state on the magnitude and pattern of pulmonary venous flow are poorly understood. METHODS AND RESULTS Fourteen anesthetized open-chest mongrel dogs were instrumented with pulmonary venous flow probes, atrial sonomicrometer crystal paris, and high-fidelity micromanometers; transesophageal Doppler echocardiography was used to obtain simultaneous pulmonary venous flow velocities. Measurements were made over a wide range of left atrial pressure obtained by either intravascular volume infusion and inferior vena caval balloon inflation (n = 8), halothane inhalation (n = 6), or phenylephrine infusion (n = 5). There was an excellent correlation for pulmonary venous systolic (J) to diastolic (K) time integral between the Doppler and flow probe signal (r = 0.94; SEE, 0.18). When left atrial pressure was increased by volume infusion, there was a significant linear relation between mean left atrial pressure and the Doppler J/K peak (r = 0.64; SEE, 3.4 mm Hg) and flow velocity-time integral ratio (r = 0.75; SEE, 2.9 mm Hg). By contrast, when left atrial pressure was elevated by halothane-induced cardiac depression, there was no correlation. The independent determinants of the pattern of pulmonary venous flow (stepwise multiple linear regression analysis) under all conditions were atrial systolic shortening, aortic systolic pressure, heart rate, and left ventricular end-systolic dimension (cumulative r = 0.80). CONCLUSIONS The pattern of pulmonary venous flow can be measured accurately with Doppler velocities and is differentially influenced by loading conditions and myocardial contractile state; in the absence of myocardial contractile dysfunction, the pattern of pulmonary venous flow may provide an estimate of left atrial pressure; and pulmonary venous flow is determined largely by atrial systolic function.
Hemoglobinopathies mimicking Hb S/β‐thalassemia: Hb S/S with α‐thalassemia and Hb S/Volga
Authors: Hong‐Yuan Luo, Matthew Heeney, Winfred C. Wang, Shawn H. Eung, Russell E. Ware, Martin H. Steinberg, David H.K. Chui
Journal: American Journal of Hematology (2006)

Abstract There are approximately 1200 known natural mutations of the human globin genes. In most clinical laboratories, the diagnosis of hemoglobin disorders is based on blood counts, hemoglobin electrophoresis, or column chromatography, which can identify common variant hemoglobins such as Hb S, C, and E, but are unable to definitively diagnose most other hemoglobin variants and thalassemia mutations. We report two unrelated cases, both thought initially to have Hb S/β‐thalassemia. Subsequent mutational analyses revealed that one is Hb S/S with compound heterozygosity for nondeletional α‐thalassemia mutations. The other is the first reported case of compound heterozygosity for Hb S and an unstable hemoglobin, Hb Volga. Correct diagnoses of these hereditary disorders are needed for prognosis and proper management and also for genetic counseling. These studies underscore the importance to correlate clinical course with laboratory diagnosis and to make DNA‐based diagnostics more widely available for patients with unusual or complicated hemoglobin disorders. Am. J. Hematol. 81:361–365, 2006. © 2006 Wiley‐Liss, Inc.
Cholera epidemic hits former Soviet states
Authors: M. Ingram
Journal: BMJ (1995)

The former Soviet Union's collapsing infrastructure has left doctors powerless in the face of an epidemic of cholera that has already claimed hundreds of lives in Ukraine and is threatening to sweep Russia as well, according to Dr Alexander Losoto, who has just returned from the Black Sea town of Nikolayev.

On his return from the contaminated area Dr Losoto said that he travelled on a train laden with fruit and vegetables into the heart of Moscow and that no sanitary inspections were carried out. He said: “The recent handful of cases of cholera in Moscow is being viewed as something of a sensation. But for those who are aware of the true state …
Superimposition of In-111 Platelet SPECT and CT/MR Imaging in Intracardiac Thrombus
Authors: Kazuyoshi Suga, Kazuya Nishigauchi, Norihiko Kume, Gouji Miura, Katsuyuki Takano, Shinji Koike, Naofumi Matsunaga, Takashi Fujii, Masutoku Matsuzaki
Journal: Clinical Nuclear Medicine (1996)

An initial attempt to superimpose in-111-labeled platelet SPECT over CT/MRI in two patients with intracardiac thrombus is presented. One patient was a 65-year-old woman with a history of mitral and tricuspid valve replacement. This patient had a large thrombus measuring 74 x 43 x 34 mm in the right atrium. Fusion imaging with CT showed increased in-111 activity on the surface of the thrombus, which was visualized as circular activity on a planar image. The other patient was a 72-year-old woman who had a thrombus measuring 17 x 14 x 12 mm at the cardiac apex. Planar imaging showed two discrete areas of abnormal in-111 activity. Superimposition of the SPECT image over MR revealed that one of these areas corresponded to the small thrombus, whereas the other related to a previous subendomyocardial biopsy. In both patients, the activity ratio of the abnormal in-111 accumulation versus the cardiac chamber on SPECT was greater than that on planar images. The correlation of in-111 platelet SPECT and CT/MRI was useful in accurately localizing the intracardiac thrombus.
Hypoxia inducible factor (HIF) in rheumatology: low O2! See what HIF can do!
Authors: T Gaber, R Dziurla, R Tripmacher, G R Burmester, F Buttgereit
Journal: Annals of the Rheumatic Diseases (2005)

Maintenance of oxygen homoeostasis is the basic principle in cell proliferation, differentiation, survival, and function in all higher organisms. The transcription factor, HIF (hypoxia inducible factor) has a central role in oxygen homoeostasis, and is indispensably linked to energy metabolism. Abnormally reduced oxygen concentrations leading to dysfunctional cell metabolism are found in rheumatoid arthritis and hence, knowledge of the molecular adaptive responses to hypoxia and the involvement of HIF in the pathogenesis of RA are interesting.
Yellow Fever on the Isthmus of Panama Under the French Régime.
Authors: W. C. Gorgas
Journal: JAMA (1907)

Shac is put up in wafers and each wafer is stated to contain 4 grains of acetanilid.While shae is sold and "pushed" by Frederick Steams & Co., Detroit, it is stated on the package to be "prepared for Steams & Curtius (
Haemophilus influenzae Prostatitis
Authors: Matthew B. Goetz
Journal: JAMA (1982)

CASES of cystitis and pyelonephritis due to<i>Haemophilus influenzae</i>have been infrequently reported since the first decade of this century.<sup>1-5</sup>Recently, we treated a patient for<i>H influenzae</i>prostatitis. This organism was identified only by its growth on chocolate agar, a medium that is not ordinarily used for routine urine cultures.<sup>6</sup> <h3>Report of a Case</h3> A 53-year-old man was in excellent health until Aug 23, 1981, when he had transient myalgias, pharyngitis, and coryza. The following day he experienced urinary urgency and frequency accompanied by suprapubic and urethral pain. On Aug 26 he had a single episode of gross hematuria. When seen at the University of Wisconsin Hospital, Madison, the next day, he denied respiratory tract symptoms, rash, arthralgias, chills, or penile discharge. He had one steady sexual partner. Medical history was remarkable for two episodes of transient hematuria 25 years earlier, following which cystoscopy results and a
Guidelines for colonoscopic screening in acromegaly are inconsistent with those for other high risk groups
Authors: A G Renehan
Journal: Gut (2003)

Caution with the use of cyclosporin in pregnancyWe read the article by Alstead and Nelson-Piercy with great interest. 1 We report a case (submitted for publication) of a woman with fulminant ulcerative colitis in the 29th week of pregnancy.Her disease was refractory to steroids, but she refused to have cyclosporin whilst pregnant.She therefore underwent an emergency Caesarean section and was given intravenous cyclosporin post-operatively.After 48 hours of treatment she developed severe hypertension with hypertensive encephalopathy and seizures.Although cyclosporin has been considered to be safe for both mother and foetus, we would like to highlight concerns that it is associated with potentially life threatening side effects.As a result, patients must be counselled thoroughly about the potential morbidity associated with this treatment and monitored closely.We agree with Dr Alstead in that cyclosporin should be used with extreme caution in pregnancy and the postnatal period.
Severe acute respiratory syndrome from the trenches, at a Singapore university hospital
Authors: Paul A Tambyah
Journal: The Lancet Infectious Diseases (2004)

The epidemiology and virology of severe acute respiratory syndrome (SARS) have been written about many times and several guidelines on the infection control and public health measures believed necessary to control the spread of the virus have been published. However, there have been few reports of the problems that infectious disease clinicians encounter when dealing with the protean manifestations of this pathogen. This is a qualitative account of some of the issues faced by an infectious disease physician when identifying and treating patients with SARS as well as protecting other healthcare workers and patients, including: identification of the chain of contagion, early recognition of the disease in the absence of a reliable and rapid diagnostic test, appropriate use of personal protective equipment, and the use of isolation to prevent super-spreading events. Many issues need to be addressed if clinicians are to be able to manage the virus should it reappear.
Building-Associated Risk of Febrile Acute Respiratory Disease
Authors: Michael J. Hodgson
Journal: JAMA (1988)

To the Editor.—  Brundage et al 1 are to be commended on their fascinating study, although they may understate its importance. Transmission of viral disease through ventilation systems has indeed been well described, but only for measles, rubella, and varicella viruses. Common features of these three viruses include high primary and secondary attack rates. The reference cited by Brundage et al concerning influenza outbreaks in penal institutions and schools 2 does not address the relationship of the epidemics with ventilation systems. Another reference 3 refers to two outbreaks of allergic lung disease, one related to a central air washer 4 and one to cafeteria flooding. 5 Their own data are therefore the first, to my knowledge, to suggest an increased frequency of respiratory tract infections from common viruses related simply to mechanical ventilation.  Two factors may contribute to the recirculation and viability of viruses in the indoor air, leading to
Implications of inflammatory changes on cervical cytology
Authors: N. O'Farrell
Journal: BMJ (1990)

leucocytes, in addition, does not automatically imply inflammation.As is known, these non- discrete criteria lend themselves to extensive interobserver variation in reporting.The statement that the study population was selected solely by returned cytology forms implies that no single observer reported the results of all of the tests.Indeed, the results were probably reported by many observers of varying ability and experience.It would have been prudent to have had a single cvtopathologist to review all of the preparations in this study.Furthermore, the fact that some slides were unsatisfactory shows that the selection was flawed.It is worth noting that many of the infections discussed may be identified from cytological preparations: Candida albicans, Gardnerella vaginalis, Trichomonas vaginalis, and herpes simplex virus.If identified these may be listed as
Effect of exercise on perfusion of collateral-dependent myocardium in dogs with chronic coronary artery occlusion.
Authors: P R Lambert, D S Hess, R J Bache
Journal: Journal of Clinical Investigation (1977)

Since the ability of mature intercoronary collateral channels to increase myocardial blood flow in response to drug-induced coronary vasodilation has been questioned, the present study was undertaken to evaluate the response of coronary collateral circulation to the stress of exercise. Studies were performed at rest and during two levels of treadmill exercise in six dogs a minimum of 6 mo after placement of an Ameroid constrictor on the left circumflex coronary artery. Regional myocardial blood flow was estimated in normally perfused anterior and predominantly collateral-dependent posterior left ventricular wall with left atrial injections of radio-nuclide-labeled microscheres 7-10 mum in diameter. At rest, heart rate was 87 +/- 7 beats/min and mean myocardial blood flow was comparable in control and collateral-dependent regions (0.96 +/- 0.13 and 0.97 +/- 0.14 ml/min-g, respectively). During exercise, heart rates increased to 180 +/- 13 and 228 +/- 14 beats/min and myocardial blood flow (MBF) in the anterior control region increased linearly with heart rate (HR), (MBF = 0.133 HR - 0.202, r = 0.88). MBF to the posterior collateral-dependent region was similarly augmented during exercise (MBF = 0.140 HR - 0.252, r = 0.89), so that the linear correlation between HR and MBF was similar for the control and collateral-dependent regions. In addition, the transmural distribution of MBF was uniform at rest and during exercise in both the anterior control and posterior collateral-dependent regions. Thus, not only could the mature intercoronary collateral vasculature supply adequate flow at rest, but when subjected to the natural stress of exercise, the increase in flow to the predominantly collateral-dependent area was similar to that in the normally perfused area.
Imaging for Sinusitis-Reply
Authors: Mahmood F. Mafee
Journal: JAMA (1993)

In Reply.  —I appreciate the comments by Drs Harrie, Howe, and Adcock on my response to a question concerning imaging methods for sinusitis.  I agree with Harrie that unfortunately, disease of the ethmoid sinuses is often missed on plain roentgenograms. It is important to realize that not only diseases of ethmoid but diseases of sphenoid, frontal, and even maxillary sinuses can be missed on a plain roentgenogram.  Harrie states that he has found  to be a valuable screening technique for this blind spot between roentgenographic and CT detection of ethmoid sinus disease.  We know that orbital and cranial complications of sinusitis are very serious. Postseptal orbital cellulitis generally is seen with signs and symptoms of chemosis, proptosis, and restricted eye movement; however, clinical examinations alone cannot distinguish early inflammation from abscess or extraconal from intraconal. 1 Although ultrasound may be used as a screening technique, most investigators agree that