tbuckley/cabot-search huggingface dataset:
Golgi phosphoprotein 2 (GOLPH2) expression in liver tumors and its value as a serum marker in hepatocellular carcinomas
Authors: Marc-Oliver Riener, Frank Stenner, Heike Liewen, Christopher Soll, Stefan Breitenstein, Bernhard Cornelius Pestalozzi, Panagiotis Samaras, Nicole Probst-Hensch, Claus Hellerbrand, Beat Müllhaupt, Pierre-Alain Clavien, Marcus Bahra, Peter Neuhaus, Peter Wild, Florian Fritzsche, Holger Moch, Wolfram Jochum, Glen Kristiansen
Journal: Hepatology (2009)

Abstract Hepatocellular carcinomas (HCCs) and bile duct carcinomas (BDCs) have a poor prognosis. Therefore, surveillance strategies including sensitive and specific serum markers for early detection are needed. Recently, Golgi Phosphoprotein 2 (GOLPH2) has been proposed as a serum marker for HCC, but GOLPH2 expression data in liver tissues was not available. Using tissue microarrays and immunohistochemistry, we semiquantitatively analyzed GOLPH2 protein expression in patients with HCC (n = 170), benign liver tumors (n = 22), BDC (n = 114) and normal liver tissue (n = 105). A newly designed sandwich enzyme-linked immunoassay (ELISA) was used to analyze GOLPH2 levels in the sera of patients with HCC (n = 62), hepatitis C virus (HCV) (n = 29), BDC (n = 10), and healthy control persons (n = 12). By immunohistochemistry 121/170 (71%) of HCC showed strong GOLPH2 expression, which was significantly associated with a higher tumor grade ( P = 0.01). A total of 97/114 (85%) BDCs showed a strong GOLPH2 expression which proved to be an independent prognostic factor for overall survival ( P &lt; 0.05). Serum levels of GOLPH2 measured by ELISA were significantly elevated in patients with HCC with underlying HCV infection (median 18 mg/L, P &lt; 0.05) and patients with BDC (median = 14.5 mg/L, P &lt; 0.01) in comparison to healthy controls (median 4 mg/L). Conclusion: GOLPH2 protein is highly expressed in tissues of HCC and BDC. GOLPH2 protein levels are detectable and quantifiable in sera by ELISA. In patients with hepatitis C, serial ELISA measurements in the course of the disease appear to be a promising complementary serum marker in the surveillance of HCC. GOLPH2 should be further evaluated as a serum tumor marker in BDC on a larger scale. (Hepatology 2009.)
Valproic Acid Bibliography
Authors: J. KIFFIN PENRY
Journal: Annals of Internal Medicine (1979)

Letters and Corrections1 April 1979Valproic Acid BibliographyJ. KIFFIN PENRY, M.D.J. KIFFIN PENRY, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-90-4-724_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: Valproic acid (Depakene®) is a newly approved drug for the treatment of seizures. Its availability is a significant advance because valproic acid is effective in many types of seizures and is relatively free of serious side effects. The Epilepsy Branch of the National Institute of Neurological and Communicative Disorders and Stroke (NINCDS) has prepared a classified bibliography on valproic acid to aid in its continued investigation and its application to clinical practice.Copies of this publication are available at no cost from the Office of Scientific and Health Reports, NINCDS, National Institutes of Health, Building 31, Room... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: J. KIFFIN PENRY, M.D.Affiliations: National Institute of Neurological and Communicative Disorders and Stroke Bethesda, MD 20014 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 April 1979Volume 90, Issue 4 Page: 724-724 Keywords Drugs Epilepsy Epileptic seizures Stroke ePublished: 1 December 2008 Issue Published: 1 April 1979 PDF downloadLoading ...
Diagnostic Value of HLA-B27 Testing in Ankylosing Spondylitis and Reiter's Syndrome
Authors: Muhammad A. Khan, Mohammad K. Khan
Journal: Annals of Internal Medicine (1982)

Typing for histocompatibility antigen (HLA)-B27 has been suggested as a clinically valuable diagnostic test for ankylosing spondylitis and Reiter's syndrome, although some decry its use for this purpose. Diagnoses can be made in most patients with these diseases on the basis of the history, physical examination, and roentgenographic findings. The B27 test cannot be used to screen an asymptomatic population to detect these diseases and should not be thought of as a routine diagnostic test. We present probability graphs derived from Bayes' theorem, which show that for certain patients the B27 test, when used properly, is of clinical value as an aid to diagnosis. Proper application of the B27 test in clinical medicine is discussed. The test result does not absolutely confirm or exclude the presence of these diseases; it merely provides a probability statement on their existence in the patient. The test is therefore most useful to physicians who understand the use of probability reasoning in clinical decision making.
Imatinib sensitivity as a consequence of a CSF1R-Y571D mutation and CSF1/CSF1R signaling abnormalities in the cell line GDM1
Authors: A Chase, B Schultheis, S Kreil, J Baxter, C Hidalgo-Curtis, A Jones, L Zhang, F H Grand, J V Melo, N C P Cross
Journal: Leukemia (2008)
CHROMOSOME ABERRATIONS AND SISTER-CHROMATID EXCHANGE IN WORKERS IN CHEMICAL LABORATORIES AND A ROTOPRINTING FACTORY AND IN CHILDREN OF WOMEN LABORATORY WORKERS
Authors: F. Funes-Cravioto, B. Kolmodin-Hedman, J. Lindsten, M. Nordenskjöld, C. Zapata-Gayon, B. Lambert, E. Norberg, R. Olin, Å. Swensson
Journal: The Lancet (1977)
Pharmacology of the high-affinity apamin receptor in rabbit heart
Authors: J. A. Schetz, P. A.V. Anderson
Journal: Cardiovascular Research (1995)

Apamin is a potent blocker of calcium-activated small conductance potassium (SK) channels in neurons, liver, skeletal muscle and ileum smooth muscle, but not in cardiac muscle. Cardiac muscle is devoid of SK channels; however, in isolated, single ventricular myocytes apamin is an extremely potent blocker of the L-type calcium current, and the anti-arrhythmic drug quinidine reverses apamin block.To characterize the receptor binding properties and pharmacology of the apamin receptor in heart.The binding properties of the apamin receptor were determined by rapid filtration of purified rabbit heart membranes.Monoiodinated apamin binds to a labile, membrane-bound protein in heart membranes at a single, high-affinity site (KD = 8.07 +/- 2.14 pM and Bmax = 686 +/- 167 fmoles/mg protein, significant run test at P = 0.05 for a one site fit). 125I-apamin binding is dose-dependently inhibited by apamin, scyllatoxin, quinidine, amiloride, as well as a variety of di- and trivalent cations that are classical blockers of L-type calcium channels (e.g. Co2+, Cd2+, Mn2+, La3+, Gd3+). The cardiac apamin receptor is also critically dependent upon pH, temperature and KCl, and co-purifies in the same membrane fraction as L-type cardiac Ca2+ channels.The apamin receptor in rabbit heart P2 membranes has pharmacological and biochemical properties in common with both an SK channel and an L-type Ca2+ channel.
THE METABOLISM OF HUMAN ERYTHROBLASTS
Authors: Walter Kempner
Journal: Journal of Clinical Investigation (1936)

The metabolism of human nucleated red blood cells has not previously been determined because of difficulties in procuring material. In most anemias the number of nucleated erythrocytes in the blood is so small in relation to the number of non-nucleated erythrocytes that the metabolism of the former is obscured by that of the latter. Moreover, it is impossible without damaging their very sensitive metabolic reactions to separate in sufficient amount the nucleated from the nonnucleated red blood cells and from the leukocytes. The opportunity recently presented itself through the kindness of the Department of Pediatrics of examining at intervals of from three to five months the blood of a patient with erythroblastic anemia who had an extremely high proportion of nucleated red blood cells, 130,000 to 364,000 erythroblasts to 1.8 to 3.5 million nonnucleated erythrocytes in 1 c.mm. of blood. The patient was an eight year old boy of Greek parentage. One sister died at 2 years with symptoms similar to the patient's. Parents and a 3 year old brother are in good health. Since 1932 the patient has been given blood transfusions for his anemia regularly every few months. Splenectomy in 1933 was not followed by any evident improvement. At the time this study was begun there were 245,000 erythroblasts and 3.25 million non-nucleated red blood cells in 1 c.mm., that is, a percentage of 7 per cent of erythroblasts in his total red cell count. The percentage of reticulocytes was below 1 per cent. The leukocytes varied between 5,000 and 7,000 per c.mm.
Intravenous Administration of Self-complementary AAV9 Enables Transgene Delivery to Adult Motor Neurons
Authors: Sandra Duque, Béatrice Joussemet, Christel Riviere, Thibaut Marais, Laurence Dubreil, Anne-Marie Douar, John Fyfe, Philippe Moullier, Marie-Anne Colle, Martine Barkats
Journal: Molecular Therapy (2009)

Therapeutic gene delivery to the whole spinal cord is a major challenge for the treatment of motor neuron (MN) diseases. Systemic administration of viral gene vectors would provide an optimal means for the long-term delivery of therapeutic molecules from blood to the spinal cord but this approach is hindered by the presence of the blood–brain barrier (BBB). Here, we describe the first successful study of MN transduction in adult animals following intravenous (i.v.) delivery of self-complementary (sc) AAV9 vectors (up to 28% in mice). Intravenous MN transduction was achieved in adults without pharmacological disruption of the BBB and transgene expression lasted at least 5 months. Importantly, this finding was successfully translated to large animals, with the demonstration of an efficient systemic scAAV9 gene delivery to the neonate and adult cat spinal cord. This new and noninvasive procedure raises the hope of whole spinal cord correction of MN diseases and may lead to the development of new gene therapy protocols in patients. Therapeutic gene delivery to the whole spinal cord is a major challenge for the treatment of motor neuron (MN) diseases. Systemic administration of viral gene vectors would provide an optimal means for the long-term delivery of therapeutic molecules from blood to the spinal cord but this approach is hindered by the presence of the blood–brain barrier (BBB). Here, we describe the first successful study of MN transduction in adult animals following intravenous (i.v.) delivery of self-complementary (sc) AAV9 vectors (up to 28% in mice). Intravenous MN transduction was achieved in adults without pharmacological disruption of the BBB and transgene expression lasted at least 5 months. Importantly, this finding was successfully translated to large animals, with the demonstration of an efficient systemic scAAV9 gene delivery to the neonate and adult cat spinal cord. This new and noninvasive procedure raises the hope of whole spinal cord correction of MN diseases and may lead to the development of new gene therapy protocols in patients.
SmithKline Beecham fined in Hungary
Authors: C. Kovac
Journal: BMJ (1997)

SmithKline Beecham Pharmaceuticals has been fined one million Hungarian florints (£3065) by the Hungarian Consumer Protection Authority and ordered to withdraw advertisements that promote vaccinations against hepatitis B.

The advertisements started appearing in August in magazines targeted at teenagers and on television. According to the national public health commissioner, Ilona Molnar, the advertisements exaggerated the …
Transmission of Herpes Simplex
Authors: George J. Pazin
Journal: JAMA (1984)

To the Editor.—  We have several objections to the article entitled Survival of Herpes Simplex Virus in Water Specimens Collected From Hot Tubs in Spa Facilities and on Plastic Surfaces, by Nerurkar et al. 1   The most important objections center around the authors' repeated statements that it is important to consider possible nonvenereal modes of transmission of herpes simplex virus [HSV] infections, that those sources [tap water and plastic surfaces] must be considered as important possible routes for nonvenereal spread of HSV, and, finally, that further epidemiologic studies will be needed to clarify the  recognized  of fomites in the spread of HSV. Neither the studies cited 2-3 nor the study reported 1 support an increasingly recognized role for fomites in the spread of genital herpes. The theoretical possibility of nonsexual transmission of genital herpes may seem reasonable to laboratory investigators, the press, the general public, or to physicians not
Electrocardiographic abnormalities and associated factors in Chinese living in Beijing and in Mauritius. The Mauritius Non-Communicable Disease Study Group.
Authors: N. Li, J. Tuomilehto, G. Dowse, K. G. Alberti, P. Zimmet, Z. Min, P. Chitson, H. Gareeboo, Y. Chonghua, D. Fareed
Journal: BMJ (1992)

OBJECTIVE--To compare the prevalence of electrocardiographic abnormalities and to evaluate the association between these abnormalities and the levels of coronary heart disease among Chinese living in different environments. DESIGN--Cross sectional surveys. SETTING--Beijing, China, and the island of Mauritius. SUBJECTS--Random samples of people aged 35-64 years in Beijing (621 men, 642 women) in 1984 and in Mauritius among Chinese (137 men, 130 women) and non-Chinese (1265 men, 1432 women) in 1987. MAIN OUTCOME MEASURES--Prevalence of electrocardiographic abnormalities suggesting coronary heart disease and of associated risk factors. RESULTS--Prevalence of electrocardiographic abnormalities suggesting coronary heart disease was significantly lower in Beijing (4.0%) than in Mauritian Chinese (24.3%) and Mauritian non-Chinese (24.5%). Mean serum concentrations of total and non-high density lipoprotein cholesterol were lower in Beijing Chinese than in Mauritian Chinese, but smoking and hypertension were slightly more prevalent. Overall, men with electrocardiographic abnormalities had higher risk factor levels than those with a normal electrocardiogram regardless of ethnic origin. CONCLUSIONS--The prevalence of coronary heart disease and associated risk factors was different among Chinese living in two different environments: in Beijing in the People9s Republic of China and in Mauritius. Chinese, who traditionally have a very low frequency of coronary heart disease, are by no means protected against coronary heart disease and other non-communicable diseases. Therefore, primary prevention of coronary heart disease is a major challenge for preventive medicine in China, as well as in many other developing countries.
Number, type, and availability of new drugs are on the increase in Europe
Authors: R. Watson
Journal: BMJ (2013)

Europe’s drugs scene is evolving as the use of established illicit substances such as heroin declines, but the number, type, and availability of new drugs continues to increase. The new challenges facing policy makers are set out in the latest annual report from the Lisbon based European Monitoring Centre for Drugs and Drug Addiction.1

The report confirms fewer new users of heroin, less injecting, and declining use of cannabis and cocaine in some European countries. However, cannabis use remains high by historical standards: some 77 million European adults have …
A thymic pathway of mouse natural killer cell development characterized by expression of GATA-3 and CD127
Authors: Christian A J Vosshenrich, Marcos E García-Ojeda, Sandrine I Samson-Villéger, Valerie Pasqualetto, Laurence Enault, Odile Richard-Le Goff, Erwan Corcuff, Delphine Guy-Grand, Benedita Rocha, Ana Cumano, Lars Rogge, Sophie Ezine, James P Di Santo
Journal: Nature Immunology (2006)
The Evaluation of Therapeutic Agents and Cosmetics
Authors: Joseph B. Jerome
Journal: JAMA (1964)

This book is based on a conference held in August, 1962, just prior to the passage of the drug amendments of 1962 and the subsequent issuance of regulations relative to investigational drugs. As a result of the late appearance of this publication and the failure to revise the papers to reflect the current situation, seven (of the 26) chapters are to a large degree outdated. Unfortunately, these are the chapters addressed to the conference theme, "Evaluation." The other chapters, principally in basic scientific areas, are uneven in content and presentation but have survived the passage of time with smaller loss in value. Even this material could have been improved by more careful editing, as for example, the legends on many graphs. The principal value of this book is to serve as a record of the conference; participants and possibly others may find it an interesting historical record of drug-evaluation procedures
Extrahepatic transcription of human C-reactive protein.
Authors: T M Murphy, L L Baum, K D Beaman
Journal: The Journal of Experimental Medicine (1991)

We synthesized and cloned cDNA from human peripheral blood mononuclear cell (PBMC) transcripts that were hybrid selected by pCRP5, a liver C-reactive protein (CRP)-specific cDNA (Woo, P.,J.R. Korenberg, and A.S. Whitehead. 1985. J.Biol. Chem. 260:13384). Three hybrid-selected cDNA clones, HScDNA1, HScDNA3, and HScDNA8, were isolated and characterized. Nucleotide sequence analysis of the 5' end of the smaller clones, HScDNA1 and HScDNA8, demonstrated that these two PBMC clones are homologous to the 3' and 5' ends, respectively, of pCRP5. Our largest clone, HScDNA3, is larger than pCRP5, extending beyond both the 5' and 3' limits of pCRP5. Therefore, HScDNA3 was coded by human PBMC and not by the hybrid selection vehicle, pCRP5. HScDNA3 lacks the intervening sequence verifying that this clone is DNA made from a PBMC mRNA and not genomic DNA. The complete nucleotide sequence revealed that HScDNA3 is greater than 99% homologous to the CRP gene. These results demonstrate that PBMC express the CRP gene. Based on our previous report, which shows that peripheral blood cells synthesize a peptide recognized by anti-CRP (Kuta, A.E., and L.L. Baum. 1986. J. Exp. Med. 164:321), in conjunction with the data presented here, we conclude that human PBMC can synthesize CRP.
The senses of the newborn.
Authors: D. A. Curnock
Journal: BMJ (1989)

also support patients' councils in psychiatric hospitals and social and recreational activities in day units.'4In London a consortium of patients, mental health workers, and carers has influenced statutory planning'" and hospital practice.'6Health professionals can learn from feedback, but patients' expressed satisfaction with services varies according to who asks the questions.Studies initiated by providers of services yield high ratings for satisfaction.Quantitative assessment of satisfaction with treatment is readily attainable,'7 even from severely disabled patients."'In the United States community mental health centres have been required by law since 1975 to evaluate patients' satisfaction.'9Results, however, may be artificially improved by low response rates and biased assessment techniques.2"2'Qualitative surveys conducted by patients' groups are more critical'6 but may also be biased: such groups set out to find and solve problems and they may overgeneralise without being representative of all patients.22Methodological factors also exaggerate this contrast between quantitative and qualitative research.2AHow can psychiatry respond to the growth in these groups?The second Griffiths report does not mention user participation directly.24Neither does the Royal College of Psychiatry's paper on community care.29But the psychiatric profession must learn more about the scope and utility of user participation and encourage it: the persistence of patients' groups seems to underlie many of the most innovative programmes in mental health care.The King's Fund has called for increased collaboration between patients, planners, and managers in mental health care.26This could improve clinical outcome, encourage agreement about priorities in treatment, and resolve an embarrassing divergence between providers and users of services.
Clonidine may relieve opiate withdrawal symptoms
Authors: May Annexton
Journal: JAMA (1978)

In cases of drug withdrawal, the cure may be little better than the disease. The use of methadone as a withdrawal agent for heroin addiction is a case in point. However, an antihypertensive drug, clonidine hydrochloride, may help to drive a wedge into this clinical paradox. According to Mark S. Gold, MD, director of research, Fair Oaks Hospital, Summit, NJ, and vice president, basic research, Psychiatric Institute of America, clonidine is the first nonopiate treatment for drug withdrawal. In both animal and double-blind crossover human studies, he says, clonidine provided "dramatic relief of subjective distress due to withdrawal." Eleven patients, addicted to opiates for six to ten years and to methadone for six to 60 months, received 5 μg/kg of clonidine or placebo orally in two doses two hours apart in the hospital. (The patients were volunteers who had expressed interest in withdrawing from methadone therapy and had fulfilled the
Royal College expresses concern over transfer of out of hours GP services
Authors: Bruno Rushforth
Journal: BMJ (2004)

Professor David Haslam, chairman of the council of the Royal College of General Practitioners, has expressed concerns to the parliamentary health select committee over the “potential crisis” in GP out of hours services when responsibility for night and weekend cover is transferred to primary care trusts on 31 December.

Commenting on the readiness of primary care trusts for their new role, Professor Haslam said, “I think there is a general underestimation of the complexity of what goes on in an out of hours consultation.”

Dr Mark Reynolds, chairman of the National Association of GP Co-operatives, voiced similar concerns: “People in primary care trusts are taking this job on with no experience of it and …
Ankylosing spondylitis: evidence for a non-HLA-B*27 protective effect
Authors: S. VAN DER LINDEN, D. VAN DER HEIJDE;, M. BROWN, P. WORDSWORTH
Journal: Annals of the Rheumatic Diseases (1998)

The question whether or not there is evidence for a non-HLA-B*27 protective effect is still open to debate.1-3 The likelihood of finding cases of ankylosing spondylitis (AS) depends on assumptions on the risk for HLA-B27 positive persons to develop the disease. Prevalence figures as low as 1.3% and 1.4% have been reported for population based studies in Zoetermeer (the Netherlands) and Busselton (Australia). These figures are based on populations where most people underwent pelvic radiography.4 5 Estimates based on completion of questionnaires and random sampling provide … Dr M A Brown.
Suicide Risk and Coping Styles in Posttraumatic Stress Disorder Patients
Authors: M. Amir, Z. Kaplan, R. Efroni, M. Kotler
Journal: Psychotherapy and Psychosomatics (1999)

&lt;i&gt;Background:&lt;/i&gt; Suicide and suicidal behavior have been found to be increased among posttraumatic stress disorder (PTSD) patients. The present study examined suicide risk and Plutchik’s coping styles in PTSD patients. &lt;i&gt;Method:&lt;/i&gt; 47 PTSD patients were compared with 42 patients with mixed non-PTSD anxiety disorders and 50 healthy control subjects, matched for age and gender, on a measure of suicide risk. &lt;i&gt;Results:&lt;/i&gt; The PTSD patients scored significantly higher than the two control groups on the suicide risk measure. Furthermore, in the PTSD group, suicide risk was significantly negatively correlated with the coping mechanisms of mapping, minimization and replacement and positively correlated with the coping style of suppression. Furthermore, the coping styles significantly explained the variance of the suicide risk measure for all three groups. &lt;i&gt;Conclusions:&lt;/i&gt; The cognitive map of PTSD patients highly resembles other populations with high suicide risk. Clinicians treating victims of traumatic events should focus on problem-solving therapies in order to help these patients deal less rigidly with everyday stresses and by this decrease the suicide risk.