tbuckley/cabot-search huggingface dataset:
Cytoplasmic receptor for glucocorticoids in lung of the human fetus and neonate.
Authors: P L Ballard, R A Ballard
Journal: Journal of Clinical Investigation (1974)

In fetal animals, glucocorticoids accelerate development of the lung and cause precocious appearance of alveolar surfactant. To determine if the human lung also can respond to corticosteroids, we examined lungs of the human fetus and neonate for both cytoplasmic binding and nuclear uptake of glucocorticoids. In slices of fetal lung incubated with [3H]dexamethasone at 2 degrees C, specific macromolecular binding occurs primarily in the "cytoplasmic" fraction. After further incubation at 37 degrees C. nearly 75% of the radioactivity localizes in the "nuclear" fraction with a concentration of 0.3 pmol/mg DNA at apparent dexamethasone saturation (47 nM). The cytoplasmic receptor binds dexamethasone in vitro with high affinity (dissociation constant = 8.9 nM), and the affinity of various other steroids correlates with their glucocorticoid potency. Receptor was present in lungs of fetuses and neonates of gestational age 12-43 wk, with a mean concentration in hysterotomy specimens of 0.24 pmol sites/mg cytosol protein. Similar binding activity was present at lower concentration in fetal liver, gut, kidney, heart, muscle, and skin. Cytoplasmic receptor was not detected in lung and liver of premature infants with respiratory distress syndrome. This deficit appears to result from increased levels of endogenous steroids (mean cortisol 45.5 micrograms/100 ml cytosol) as well as inactivation of receptor secondary to the illness. Thus, the lung of the human fetus and neonate contains the receptor mechanism necessary for direct responsiveness to glucocorticoids. These findings support the potential usefulness of these hormones in prevention of respiratory distress syndrome in the premature infant.
Abstract 1271: Human non small cell lung cancer tissue has enhanced expression of pyruvate carboxylase, but not glutaminase
Authors: Matthew P. Fox, Michael Bousamra, Stephen P. Slone, Andrew N. Lane, Teresa W-M Fan
Journal: Cancer Research (2011)

Abstract Altered cellular metabolism in tumors is well established, particularly an increased rate of aerobic glycolysis [1]. However, increased glycolysis alone is insufficient to sustain the enhanced cell growth and anabolic metabolism characteristic of cancer. Another key source of these precursors is the citric acid cycle (CAC). Removal of CAC intermediates for anabolism requires replenishment by other sources; these anaplerotic reactions are vital to cancer development. The two major pathways of anaplerosis are glutaminolyis (deamidation and transamination of glutamine to 2-oxoglutarate) [2] and carboxylation of pyruvate to oxaloacetate (OAA), catalyzed by pyruvate carboxylase (PCB) [2]. We have coupled 13C-labeled glucose tracer with stable isotope resolved metabolomics (SIRM), gene microarray, and western blotting to track the anaplerotic PCB pathway in human lung cancer patients. The resected cancer tissue showed increased flux through the PCB pathway, along with increased transcription of the PCB gene and PCB protein expression compared with the adjacent benign lung tissue [3]. We have extended these findings to 50 paired tissue samples, and have correlated them with histological and clinical data. The protein expression of PCB was on average ten-fold higher in the tumor than in the benign lung tissue (p=0.00001, paired t-test). The normalized protein expression correlated with the fraction of the tumor that was identified as cancer cells, indicating that the increased expression was from the cancer cells rather than other lung cell types. Glutaminase was active in the lung tissue, but its expression was not different from, or lower in the tumors than in the benign lung tissue. These data indicate that in NSCLC, PCB is important in anaplerosis and tumor metabolism. The balance between glycolysis and the choice of different anaplerotic pathways may depend on the tissue types. References 1. Gillies, R.J. and R.A. Gatenby, Adaptive landscapes and emergent phenotypes: why do cancers have high glycolysis? Journal of Bioenergetics and Biomembranes, 2007. 39(3): p. 251-257. 2. Portais, J.C., P. Voisin, M. Merle, and P. Canioni, Glucose and glutamine metabolism in C6 glioma cells studied by carbon 13 NMR. Biochimie, 1996. 78(3): p. 155-164. 3. Fan, T.W., A.N. Lane, R.M. Higashi, M.A. Farag, H. Gao, M. Bousamra, and D.M. Miller, Altered Regulation of Metabolic Pathways in Human Lung Cancer Discerned by 13C Stable Isotope-Resolved Metabolomics (SIRM)) Molecular Cancer 2009. 8 p. 41. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1271. doi:10.1158/1538-7445.AM2011-1271
THE INCIDENCE AND OUTCOME OF DEVICES ‘STUCK’ IN THE CORONARY ARTERY DURING PCI: A TOYOHASHI EXPERIENCE
Authors: Masashi Kimura, Etsuo Tsuchikane, Takahiko Suzuki, Tomohiko Teramoto, Kenya Nasu, Maoto Habara, Mitsuyasu Terashima, Yasushi Asakura, Yoshihisa Kinoshita, Tetsuo Matsubara
Journal: Journal of the American College of Cardiology (2013)
Atrial fibrillation: Edited by D. Martin, M.E. Mendelsohn, R.M. John and J. Loscaizo. 146 pp., 35.00 (hardback), Cambridge, MA, USA: Blackwell Scientific Inc., 1993. ISBN 0-86542-100-5
Authors: L. Szekeres
Journal: Cardiovascular Research (1995)

Atrial fibrillation: Edited by D. Martin, M.E. Mendelsohn, R.M. John and J. Loscaizo. 146 pp., £35.00 (hardback), Cambridge, MA, USA: Blackwell Scientific Inc., 1993. ISBN 0-86542-100-5 Laszlo Szekeres Laszlo Szekeres Szeged, Hungary Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 30, Issue 1, July 1995, Pages 159–160, https://doi.org/10.1016/S0008-6363(95)90144-2 Published: 01 July 1995
Abstract 5282: Promotion of skin tumorigenesis by deletion of Ppp6c gene
Authors: Katsuhisa Hayashi, Honami Ogoh, Toshio Watanabe, Nobuhiro Tanuma, Hiroshi Shima
Journal: Cancer Research (2014)

Abstract Protein phosphatase-6 (PP6) is a member of type-2A protein-Ser/Thr phosphatases, and reportedly regulates several cellular functions including DNA damage responses, NF-kB activation, and mitotic spindle formation. Additionally, some recent papers reported somatic mutations of PPP6c gene, encoding the catalytic subunit of PP6, in about 10 % of melanoma patients, suggesting its potential role as either driver or suppressor of melanoma development. In this study, we generated mice, in which the PPP6C gene is deleted in keratinocyte-specific manner (PP6-KKO). PP6-KKO mice were predisposed to formation of skin tumor upon DMBA-treatment. Our results demonstrate PPP6c has a tumor-suppressive function at least in skin tumorigenesis. Citation Format: Katsuhisa Hayashi, Honami Ogoh, Toshio Watanabe, Nobuhiro Tanuma, Hiroshi Shima. Promotion of skin tumorigenesis by deletion of Ppp6c gene. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5282. doi:10.1158/1538-7445.AM2014-5282
Role of In-111 Labeled CYT-103 Immunoscintigraphy in the Evaluation of Patients With Recurrent Colorectal Carcinoma
Authors: ADELINE MARKOWITZ, KHALID SALEEMI, LEONARD M. FREEMAN
Journal: Clinical Nuclear Medicine (1993)

An immunoconjugate of the whole antibody, B72.3, has been approved by the FDA. It is labeled with indium-111, which allows imaging studies intended for the diagnosis and staging of colorectal and recurrent ovarian carcinoma. The new diagnostic imaging agent (CYT-103), Oncoscint CR/OV, has found particular use in evaluating patients with recurrent carcinoma. The three major indications for the study are: 1) occult disease--unexplained rise in serum tumor markers (e.g. CEA), with negative work-up including CT; 2) known local recurrence (e.g. liver or rectal area)--surgery is planned, but there is a need to rule out other areas of involvement; 3) clarify equivocal CT or MRI finding--to distinguish whether an abnormality is due to recurrent tumor or scar tissue, fibrosis, or unopacified bowel loop. Oncoscint has proven to be more sensitive than CT in the detection of disease in the pelvis and extrahepatic abdomen. CT remains the modality of choice for the detection of liver metastases. The combined sensitivity of the two modalities is 88% on a per-patient basis.
Assessment of biliary fibrosis by transient elastography in patients with PBC and PSC
Authors: Christophe Corpechot, Ahmed El Naggar, Armelle Poujol-Robert, Marianne Ziol, Dominique Wendum, Olivier Chazouillères, Victor de Lédinghen, Daniel Dhumeaux, Patrick Marcellin, Michel Beaugrand, Raoul Poupon
Journal: Hepatology (2006)

Noninvasive measurement of liver stiffness with transient elastography has been recently validated for the evaluation of hepatic fibrosis in chronic hepatitis C. The current study assessed the diagnostic performance of liver stiffness measurement (LSM) for the determination of fibrosis stage in chronic cholestatic diseases. One hundred one patients with primary biliary cirrhosis (PBC, n = 73) or primary sclerosing cholangitis (PSC, n = 28) were prospectively enrolled in a multicenter study. All patients underwent liver biopsy (LB) and LSM. Histological and fibrosis stages were assessed on LB by two pathologists. LSM was performed by transient elastography. Efficiency of LSM for the determination of histological and fibrosis stages were determined by a receiver operating characteristics (ROC) curve analysis. Analysis failed in six patients (5.9%) because of unsuitable LB (n = 4) or LSM (n = 2). Stiffness values ranged from 2.8 to 69.1 kPa (median, 7.8 kPa). LSM was correlated to both fibrosis (Spearman's ρ = 0.84, P &lt; .0001) and histological (0.79, P &lt; .0001) stages. These correlations were still found when PBC and PSC patients were analyzed separately. Areas under ROC curves were 0.92 for fibrosis stage (F) ≥2, 0.95 for F ≥ 3 and 0.96 for F = 4. Optimal stiffness cutoff values of 7.3, 9.8, and 17.3 kPa showed F ≥ 2, F ≥ 3 and F = 4, respectively. LSM and serum hyaluronic acid level were independent parameters associated with extensive fibrosis on LB. In conclusion , transient elastography is a simple and reliable noninvasive means for assessing biliary fibrosis. It should be a promising tool to assess antifibrotic therapies in PBC or PSC. (Hepatology 2006;43:1118–1124.)
Neonatal hyperbilirubinemia associated with Southeast Asian ovalocytosis
Authors: Vichai Laosombat, Supaporn Dissaneevate, Chayanon Peerapittayamongkol, Masafumi Matsuo
Journal: American Journal of Hematology (1999)

We report, herein, an infant who is twin A of a dizygotic twin, with premature birth and both twins having hemoglobin (Hb) E heterozygosity. Twin A who had Southeast Asian ovalocytosis (SAO) developed neonatal jaundice at the age of 2 days and needed phototherapy at the age of 3 days. The microbilirubin level was rapidly rising up to 535.2 μmol/L (31.3 mg/dl) with the hematocrit value of 38% at the age of 4 days prior to exchange blood transfusion. Exchange blood transfusion was done by 220 ml of O, Rh positive packed red blood cell reconstituted with 180 ml of O, Rh positive fresh plasma to lower the bilirubin level. Twin A received phototherapy from about 8 hr prior to exchange blood transfusion until 3 days later. Twin B, who did not have SAO, developed neonatal hyperbilirubinemia and needed only phototherapy. Twin A received a deletion of 27 basepairs in the erythroid band 3 gene and Hb E heterozygosity from his father. Am. J. Hematol. 60:136–139, 1999. © 1999 Wiley-Liss, Inc.
Isolation and characterization of granulocyte lysosomal proteins and study of their effects on the clotting system
Authors: John C. Herion, John R. Bucher, Ralph Penniall, Richard I. Walker, Mary Baker, Harold R. Roberts
Journal: American Journal of Hematology (1979)

Abstract Lysosomes (granules) of rabbit PMN leukocytes were extracted with either HCl or H 2 SO 4 , and the extracts were chromatographed over Sephadex to separate protein constituents. Some of the low molecular weight cationic proteins homogeneous on SDS PAGE (8% and 12.5% gels) were characterized by electrophoretic mobility in acid gels and by amino acid analysis. A 3,700 dalton polypeptide, rich in arginine and cysteine, prolonged the partial thromboplastin time of normal plasma. In low concentration, this protein shortened the clotting time of pure fibrinogen by thrombin. In high concentration this lysosomal cationic protein precipitated fibrinogen from solution; no fibrinopeptides were released to suggest cleavage of fibrinogen. Fibrinolytic protease activity was detected in crude H 2 SO 4 extracts but not in crude HCl extracts. Two separate plasminogen activators, differing from kallikrein or prekallikrein, were isolated from the H 2 SO 4 lysosomal extract and were partially characterized; neither exhibited proteolytic activity on fibrinogen free of plasminogen.
Clinical Lecture on Cholera and its Treatment
Authors: G. Johnson
Journal: BMJ (1866)

<h3>Abstract</h3> Novel coronavirus pneumonia (NCP) is an emerging, highly contagious community acquired pneumonia (CAP) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Highly efficient and accurate microbiological laboratory assay is essential to confirm the SARS-CoV-2 infection, rule out other pathogens that can cause CAP, and monitor secondary infections. Here, we enrolled and provide microbiological analysis for 129 suspected and 52 transferred confirmed NCP patients hospitalized in the First Affiliated Hospital of University of Science and Technology of China (USTC) from Jan 21 to Feb 29, 2020. By analyzing the dual swab samples (sputum and pharyngeal) from 129 suspected patients with realtime RT-PCR, we confirmed 33 SARS-CoV-2 infections, with two co-infection cases with adenovirus or rhinovirus. We also used multiplex PCR to detect 13 common respiratory tract pathogens in 96 non-NCP patients, and found that 30 patients (31.25%) were infected with at least one respiratory tract pathogen that may cause CAP. Further, we performed bacterial and fungal cultures as well as fungal serologic tests and found that there is no secondary bacterial/fungal infections in confirmed NCP patients. Our studies suggest that, during the epidemic of NCP in Anhui province, there was a certain proportion of infection and co-infection of other common pathogens of CAP, and the secondary bacterial and fungal infection is not detectable in NCP patients. In comparison with SARS-CoV-2 detection alone, this optimized strategy combining multiple pathogen detection for identification of NCP and other CAP patients as well as cultures and serologic tests for confirmed patients increases the diagnosis efficiency and facilitates the personalized medication.
Physical properties of five grain dust types.
Authors: C B Parnell, D D Jones, R D Rutherford, K J Goforth
Journal: Environmental Health Perspectives (1986)

Physical properties of grain dust derived from five grain types (soybean, rice, corn, wheat, and sorghum) were measured and reported. The grain dusts were obtained from dust collection systems of terminal grain handling facilities and were assumed to be representative of grain dust generated during the handling process. The physical properties reported were as follows: particle size distributions and surface area measurements using a Coulter Counter Model TAII; percent dust fractions less than 100 micron of whole dust; bulk density; particle density; and ash content.
Danazol and Thyroid Function
Authors: JACOBO WORTSMAN
Journal: Annals of Internal Medicine (1979)

Letters1 August 1979Danazol and Thyroid FunctionJACOBO WORTSMAN, M.D., JACK HIRSCHOWITZ, M.D., NORMAN SOLER, M.D.JACOBO WORTSMAN, M.D.Search for more papers by this author, JACK HIRSCHOWITZ, M.D.Search for more papers by this author, NORMAN SOLER, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-91-2-321_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: Dr. Spaulding has reported in the May 1979 issue (1) a patient with hereditary angioedema who developed a rise in the level of creatine phosphokinase during treatment with danazol. We have recently reported our experience with this drug in 19 patients with endometriosis (2) but our total experience to date covers 27 patients. When using danazol in doses ranging from 400 to 800 mg daily, clinically significant side-effects were few and affected only three women. One patient developed severe acne, another a transient rash, and a third patient complained of hot flashes and a decrease in the...References1. SPAULDING W. Myalgia and elevated creatine phosphokinase with Danazol® in hereditary angioedema. Ann Intern Med. 1979;90:854. Letter. LinkGoogle Scholar2. HIRSCHOWITZSOLERWORTSMAN JNJ. Sex steroid levels during treatment of endometriosis. Obstet Gynecol. 1979. In press. MedlineGoogle Scholar3. HIRSCHOWITZSOLERWORTSMAN JNJ The galactorrhea-endometriosis syndrome. Lancet. 1970;1:896-8. Google Scholar4. POTTS G. Pharmacology of danazol. J Int Med Res. 1977; 5(suppl 3):1-14. MedlineGoogle Scholar5. GOLDMANMATZMORTIMERFREEMAN JRRR. High elevations of creatine phosphokinase in hypothyroidism. An isoenzyme analysis. JAMA. 1977;238:325-6. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: JACOBO WORTSMAN, M.D.; JACK HIRSCHOWITZ, M.D.; NORMAN SOLER, M.D.Affiliations: Southern Illinois University School of Medicine Springfield, IL 62708 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byDanazol and Thyroid FunctionROBERT L. BARBIERI, M.D. 1 August 1979Volume 91, Issue 2Page: 321-321KeywordsAcneCreatineDrugsHereditary angioedemaRashesThyroid ePublished: 1 December 2008 Issue Published: 1 August 1979 PDF downloadLoading ...
Prosthetic valve endocarditis.
Authors: J L Gayet, J Delaye, J Etienne, L D Gruer, J Fleurette
Journal: BMJ (1983)

Prosthetic valve infective endocarditis (PVE) remains a devastating complication of valve replacement resulting in valvular and paravalvular destruction leading to heart failure, embolism, sepsis and ultimately death. Surgical management is frequently required for PVE due to the resistant nature of infection of prosthetic materials and the fact that infection more commonly extends beyond the affected valve proper, resulting in abscess and fistula formation. The mainstay of PVE surgery is complete debridement of infective materials and repair or replacement of all damaged structures including any abscess or fistulous connections and re-replacement of valves. This chapter provides an overview of prosthetic valve endocarditis along with a review of our surgical approach to aortic, mitral and tricuspid prosthetic valve infective endocarditis with the use of demonstrative cases.
Proliferation of human HCC cells and chemically induced mouse liver cancers requires JNK1-dependent p21 downregulation
Authors: Lijian Hui, Kurt Zatloukal, Harald Scheuch, Ewa Stepniak, Erwin F. Wagner
Journal: Journal of Clinical Investigation (2008)

JNK proteins have been shown to be involved in liver carcinogenesis in mice, but the extent of their involvement in the development of human liver cancers is unknown. Here, we show that activation of JNK1 but not JNK2 was increased in human primary hepatocellular carcinomas (HCCs). Further, JNK1 was required for human HCC cell proliferation in vitro and tumorigenesis after xenotransplantation. Importantly, mice lacking JNK1 displayed decreased tumor cell proliferation in a mouse model of liver carcinogenesis and decreased hepatocyte proliferation in a mouse model of liver regeneration. In both cases, impaired proliferation was caused by increased expression of p21, a cell-cycle inhibitor, and reduced expression of c-Myc, a negative regulator of p21. Genetic inactivation of p21 in JNK1-/- mice restored hepatocyte proliferation in models of both liver carcinogenesis and liver regeneration, and overexpression of c-Myc increased proliferation of JNK1-/- liver cells. Similarly, JNK1 was found to control the proliferation of human HCC cells by affecting p21 and c-Myc expression. Pharmacologic inhibition of JNK reduced the growth of both xenografted human HCC cells and chemically induced mouse liver cancers. These findings provide a mechanistic link between JNK activity and liver cell proliferation via p21 and c-Myc and suggest JNK targeting can be considered as a new therapeutic approach for HCC treatment.
MRI-GUIDED APPROACH TO LOCALIZE AND ABLATE GAPS IN REPEATED ATRIAL FIBRILLATION ABLATION PROCEDURE: A PILOT STUDY
Authors: Felipe Bisbal, Esther Guiu, Antonio Berruezo, Pilar Cabanas, Susana Prat-Gonzalez, Cesar Garrido, David Andreu, Juan Fernandez-Armenta, Barbara Vidal, Teresa M. Caralt, Josep Brugada, Lluis Mont
Journal: Journal of the American College of Cardiology (2013)
Eccrine porocarcinoma with melanocyte colonization
Authors: None Nakanishi, None Matsuno, None Shimoda, None Wada, None Yamazaki, None Yamamoto, None Hirohashi
Journal: British Journal of Dermatology (1998)

We describe a 74-year-old Japanese man with a pigmented eccrine porocarcinoma (malignant eccrine poroma). The patient had a brown-black nodule measuring 9 × 7 mm on his leg. Microscopically, the tumour, which had invaded the reticular dermis, was composed of poroid cells with large and small intracytoplasmic lumina. The tumour cells showed cytological pleomorphism and frequent, often abnormal, mitotic figures. Many dendritic melanocytes containing melanin were intermingled with the tumour cells. The poroid cells were diffusely positive for cytokeratin, while the dendritic cells containing melanin were positive for S-100 protein and HMB-45. Ten months of follow-up revealed no recurrence or metastasis of the tumour.
Skin testing of the pruritogenic activity of histamine and cytokines (interleukin-2 and tumour necrosis factor-α) at the dermal-epidermal junction
Authors: U. DARSOW, E. SCHAREIN, B. BROMM, J. RING
Journal: British Journal of Dermatology (1997)

Cytokines have been proposed as histamine-independent itch mediators. To investigate this hypothesis, single doses of interleukin-2 (IL-2, 10 MU/mL) and tumour necrosis factor alpha (TNF-alpha, 10 micrograms/mL) were delivered to the epidermis of 10 healthy volunteers with a controlled skin-prick model; 1% histamine and solvent controls were included in a double-blind, randomized crossover design. Itch ratings (computerized visual analogue scale) were obtained every 20 s for 15 min and cutaneous reactions (weal, flare and temperature) were measured. Reactions were also recorded after 2, 24 and 48 h. The mean itch ratings were: histamine 35.5, IL-2 3.3 (both P < 0.01 compared with control), TNF-alpha 1.6 and solvent control 1.75 (not significant). Weal and flare occurred only with histamine. In two volunteers, an inflammatory papule with transient pruritus developed 12-18 h after applying IL-2. In conclusion, IL-2 showed a rapid, low pruritogenic effect, which may be followed by an inflammatory response. TNF-alpha induced no itching in this setting. Skin-prick testing with appropriate doses of potential pruritogens provides a safe and sensitive model for further chemoreceptor studies.