tbuckley/cabot-search huggingface dataset:
Secretion of Apolipoprotein E From Macrophages Occurs via a Protein Kinase A– and Calcium-Dependent Pathway Along the Microtubule Network
Authors: Maaike Kockx, Dongni Lily Guo, Thierry Huby, Philippe Lesnik, Jason Kay, Tharani Sabaretnam, Eve Jary, Michael Hill, Katharina Gaus, John Chapman, Jennifer L. Stow, Wendy Jessup, Leonard Kritharides
Journal: Circulation Research (2007)

Macrophage-specific expression of apolipoprotein (apo)E protects against atherosclerosis; however, the signaling and trafficking pathways regulating secretion of apoE are unknown. We investigated the roles of the actin skeleton, microtubules, protein kinase A (PKA) and calcium (Ca2+) in regulating apoE secretion from macrophages. Disrupting microtubules with vinblastine or colchicine inhibited basal secretion of apoE substantially, whereas disruption of the actin skeleton had no effect. Structurally distinct inhibitors of PKA (H89, KT5720, inhibitory peptide PKI(14-22)) all decreased basal secretion of apoE by between 50% to 80% (P<0.01). Pulse-chase experiments demonstrated that inhibition of PKA reduced the rate of apoE secretion without affecting its degradation. Confocal microscopy and live cell imaging of apoE-green fluorescent protein-transfected RAW macrophages identified apoE-green fluorescent protein in vesicles colocalized with the microtubular network, and inhibition of PKA markedly inhibited vesicular movement. Chelation of intracellular calcium ([Ca2+]i) with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetate-acetoxymethyl ester (BAPTA-AM) inhibited apoE secretion by 77.2% (P<0.01). Injection of c57Bl6 apoE+/+ bone marrow-derived macrophages into the peritoneum of apoE-/- C57Bl6 mice resulted in time-dependent secretion of apoE into plasma, which was significantly inhibited by transient exposure of macrophages to BAPTA-AM and colchicine and less effectively inhibited by H89. We conclude that macrophage secretion of apoE occurs via a PKA- and calcium-dependent pathway along the microtubule network.
Notification of drug misuse--whose responsibility?
Authors: J D Knox
Journal: BMJ (1984)

1091 patients, which are used for purposes of comparison, have been carried over from the data previously thrice published.1 3 No mention is made of this in this article.This illustrates a problem not raised in your leading article.Multiple reporting of this kind may mislead readers or reviewers into regarding repetitive publications as cumulative evidence.Two of the reports2 3 were quoted in one review as forming part of "formidable" scientific evidence in favour of a particular point.5Another reviewer6 cites two of the reports1 3 as though they were separate items of scientific evidence.
276 Antigen-specific humoral and cellular immune responses during treatment of adenosine deaminase deficient severe combined immunodeficiency (ADA-SCID) with polyethylene glycol-modified bovine adenosine deaminase (PEG-ADA)
Authors: J.L. Claassen, R.H. Kobayashi, A.L. Kobayashi, M.S. Hershfield, R.I. Schiff, R.H. Buckley
Journal: Journal of Allergy and Clinical Immunology (1988)
Effect of early diastolic loading on myocardial relaxation in the intact canine left ventricle.
Authors: S Nikolic, E L Yellin, K Tamura, T Tamura, R W Frater
Journal: Circulation Research (1990)

Early transmitral flow (MiF) patterns depend strongly on the rate of fall of measured left ventricular pressure (Pm) determined by both the active decay of pressure (Pa) due to myocardial relaxation and the increase in pressure due to stretch of passive elements during filling (Pp). This study was designed to uncouple passive forces from deactivation in order to reveal the instantaneous rate and duration of myocardial relaxation. We assumed a parallel combination of passive and active elements: Pm(t,V) = Pa(t) + Pp(V), with no constraints on the form of Pa(t), where t is time and V is ventricular volume. Pp(V) was determined by a retrospective analysis of data obtained in 11 anesthetized dogs instrumented for volume clamping with a remote-controlled mitral valve, with left atrial and left ventricular micromanometers, and with an electromagnetic probe to measure MiF. The passive pressure-volume relation (both positive and negative portions) was determined by clamping at end-systolic volume or after various filling volumes, and fit to logarithmic functions. Pp(t) was then calculated from Pp(V) and V(t) (integral of MiF). Time to end relaxation (Ter) was defined as time when Pa = 0. During isovolumic relaxation, when dPp/dt = 0, dPm/dt is equal to the relaxation rate, dPa/dt. In completely isovolumic relaxations, asymptote P infinity = -7 +/- 6 mm Hg (thus, Pa is 7 mm Hg greater than Pm) and Ter = 40 +/- 15 msec, compared with 175 +/- 53 msec during normal filling. In high versus low inotropic state, Pa at the beginning of filling was greater (18.1 +/- 6.1 vs. 12.2 +/- 3.9 mm Hg), and Ter was shorter (170 +/- 42 vs. 228 +/- 43 msec). Active pressure Pa(t) during filling is not an exponential function, and at any time, it was always greater after filling than in nonfilling beats, which indicates an increase in the relaxation duration. We conclude that myocardial relaxation is modulated by filling, which slows its rate and increases its duration, and is therefore a function of both ventricular volume and time. Such a mechanism may have an important role in regulating the diastolic pressure-volume relation.
Diagnostic Testing and Immunotherapy for Allergy
Authors: Doris J. Rapp
Journal: JAMA (1988)

To the Editor.—  I was rather surprised to see all the inaccuracies in the COUNCIL REPORT that was published inTHE JOURNAL.  In the second paragraph, the report states that provocation tests use small volumes of allergens (usually ∓0.01 mL).  usual volume is actually 0.05 mL rather than 0.01; however, 0.01 mL may occasionally be used.  At the end of that same paragraph, it says, The end point is a dose that produces either symptoms or a wheal that enlarges 2 mm or more in diameter within 15 minutes. That is not the end point. In provocation testing, this is the dilution that provokes symptoms.  provocation/neutralization end point is the dilution that eliminates a patient's symptoms, and this is called theneutralization dosage.  In the fifth paragraph of the article, it says, Many investigators have attempted to perform controlled studies of these procedures, but until now none of
Ileocolonic Problems after Cadaveric Renal Transplantation
Authors: S. J. A. Powis, A. D. Barnes, P. Dawson-Edwards, H. Thompson
Journal: BMJ (1972)

Fatal colonic complications in five patients after cadaveric renal transplantation were all due to ischaemia of the bowel wall, which in two cases had led to multiple perforation. In two the lesions involved the whole colon and in three they were segmental.
The position of health in sustainable development negotiations: a survey of negotiators and review of post-Rio+20 processes
Authors: Michael W Kalmus Eliasz, Neil De Laplante, Anneleen Boel, Gerald J Makuka, Kimberly G Williams, Rispah J Walumbe, Laura Bertani, Claudel Petrin-Desrosiers, Aliye Runyan, Elizabeth Wiley, Usman Mushtaq, Arthur Mello, Sudvhir Singh, Jonathan Meldrum, Roopa Dhatt
Journal: The Lancet (2013)
The French human Salmonella surveillance system: evaluation of timeliness of laboratory reporting and factors associated with delays, 2007 to 2011
Authors: G Jones, S Le Hello, N Jourdan-da Silva, V Vaillant, H de Valk, F  X Weill, Y Le Strat
Journal: Eurosurveillance (2014)

Binary file ES_Abstracts_Final_ECDC.txt matches
Convulsions in Childhood
Authors: Hanan Costeff
Journal: New England Journal of Medicine (1965)

IN 1962 I undertook a survey of the incidence of seizures and anemia in childhood, on the suspicion that the two were associated. This hypothesis remained unproved, but two unexpected findings emerged from the study. The first was the remarkably high incidence of early convulsive episodes found in a normal population. The second was the large proportion of these seizures for which no medical attention had ever been sought. Analysis of these and other findings led to a view of childhood seizures and their treatment different from the one that dominates the recent literature. It seems worthwhile to present this . . .
EFFECT OF DICHLOROMETHYLENE DIPHOSPHONATE IN PAGET'S DISEASE OF BONE AND IN HYPERCALCÆMIA DUE TO PRIMARY HYPERPARATHYROIDISM OR MALIGNANT DISEASE
Authors: DavidL. Douglas, R. Graham G, ChristopherJ. Preston, MichaelA. Prenton, Tom Duckworth, JohnA. Kanis, F. Eric Preston, J. Stuart Woodhead
Journal: The Lancet (1980)
HbA1c Levels Increase in Patients with Non-Dipper Hypertension without Diabetes Mellitus
Authors: Salaheddin Akçay, Mehmet Ali Kobat, Fatih Inci, Zulfu Birkan, Mustafa Yıldız, Necati Dagli, Nurullah Cetin, Ali Riza Bilge
Journal: Journal of the American College of Cardiology (2013)
Influences of variation in total energy intake and dietary composition on regulation of fat cell lipolysis in ideal-weight subjects.
Authors: H Kather, E Wieland, A Scheurer, G Vogel, U Wildenberg, C Joost
Journal: Journal of Clinical Investigation (1987)

Weight-maintaining fat-rich, "prudent," carbohydrate-rich, as well as energy-restricted diets (300 kcal/d) were fed in succession for 7 d to 12 healthy males of ideal body weight under metabolic ward conditions. At the end of each period isolated fat cells were prepared from subcutaneous abdominal adipose tissue and incubated in vitro in the absence or presence of adenosine deaminase, either alone or in combination with various lipolytic or antilipolytic hormones and agents. Variations in total energy intake and dietary composition had characteristic and specific effects on fat cell lipolysis in vitro. High carbohydrate and prudent diets resulted in low rates of nonstimulated glycerol release and impaired insulin action in the presence of adenosine deaminase (320 mU/ml). High-fat and energy restricted diets were characterized by high rates of nonstimulated glycerol release. Sensitivity of antilipolysis to insulin and prostaglandin E2 was 10 to 200 times lower respectively on energy-restricted than on fat-rich diets. The effects of alpha 2- and beta-adrenergic catecholamines and of N6-phenylisopropyladenosine were not affected by the preceding diets.
Family doctors share vision of a "medical home" for every Canadian
Authors: B. Kermode-Scott
Journal: BMJ (2009)

Canada’s family doctors say that every citizen should have access to a family practice that acts as their personal “medical home” to meet their medical needs. Currently, about four million Canadians (12% of the population) do not have a family doctor.

The College of Family Physicians of Canada, which represents more than 22 000 members, described the concept of a patient centred medical home in a discussion paper issued on 29 October 2009. 

The college’s vision is that the Canadian medical home would be a medical office or clinic where patients would have a personal family doctor who provides and directs their medical care and allows patients timely access to team based care supported by electronic medical …
Abstract 1562: PYK2 and FAK in breast cancer
Authors: Ling Zhai, Rongbao Li, Zhican Qu
Journal: Cancer Research (2010)

Abstract Proliferation and invasion of malignant tumor cells are mediated by multiple events, including activation of focal adhesion signalings and cytoskeleton remodeling. Focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (Pyk2) are cytoplasmic tyrosine kinases identified as key mediators in regulation of cytoskeleton proteins in focal adhesion. Pyk2 were found to be overexpressed in Glioblastoma and played a central role in brain tumor invasion. Studies also showed that FAK increased expression and activation in various human cancers. Recently, studies with patients’ tumor samples have demonstrated that Pyk2 and FAK are co-overexpressed in over 70% human breast cancers from the very early disease stage of in situ carcinoma to the advanced metastatic stage. Pyk2 was also involved in the cytoskeleton changes initiated by heregulin through the ErbB-2 receptor in breast cancer cell lines. To further investigate role of Pyk2 and FAK in breast cancer progression and metastasis, we studied protein expression and activation of Pyk2 and FAK, as well as their substrates and interacting proteins in human breast cancer and normal breast epithelial cell lines. Our results showed that activation of both Pyk2 and FAK are marked increased in breast cancer cell lines in comparison with normal breast epithelial cells. In addition, serum stimulation was able to further activate Pyk2 and FAK and to increase tyrosine phosphorylation of their substrates and interacting proteins, including paxillin, Src, and p130CAS. The activated Pyk2 and FAK formed protein complex with paxillin, Src, and p130CAS. Our results and other published studies support the cooperative function of Pyk2 and FAK in breast cancer progression and suggest that dual inhibition of Pyk2 and FAK is a potential therapeutic approach for invasive breast cancer. Lead compounds of Pyk2 and FAK inhibitors have been identified by Southern Research drug discovery team and will be further evaluated in vitro and in vivo as potential breast cancer treatment. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1562.
Autosomal Recessive Cerebellar Ataxia Type 3 Due to<i>ANO10</i>Mutations
Authors: Mathilde Renaud, Mathieu Anheim, Erik-Jan Kamsteeg, Martial Mallaret, Fanny Mochel, Sascha Vermeer, Nathalie Drouot, Jean Pouget, Claire Redin, Emmanuelle Salort-Campana, Hubertus P. H. Kremer, Corien C. Verschuuren-Bemelmans, Jean Muller, Hans Scheffer, Alexandra Durr, Christine Tranchant, Michel Koenig
Journal: JAMA Neurology (2014)

ANO10 mutations have been reported to cause a novel form of autosomal recessive cerebellar ataxia (ARCA). Our objective was to report 9 ataxic patients carrying 8 novel ANO10 mutations to improve the delineation of this form of ARCA and provide genotype-phenotype correlation.The ANO10 gene has been sequenced in 186 consecutive patients with ARCA. The detailed phenotype of patients with ANO10 mutations was investigated and compared with the 12 previously reported cases. The mean age at onset was 33 years (range, 17-43 years), and the disease progression was slow. Corticospinal tract signs were frequent, including extensor plantar reflexes and/or diffuse tendon reflexes and/or spasticity. No patient in our series had peripheral neuropathy. Magnetic resonance imaging of the brains of our patients revealed marked cerebellar atrophy. The most frequent mutation, a mononucleotide expansion from a polyA repeat tract (c.132dupA) that causes protein truncation, was never observed in homozygosity. Only 2 truncating mutations were reported in homozygosity, one of which (c.1150-1151del) was associated with juvenile or adolescent onset and mental retardation, whereas we show that the presence of at least 1 missense or in-frame mutation is associated with adult onset and slow progression.An ANO10 mutation is responsible for ARCA that is mainly characterized by cerebellar atrophy and lack of peripheral neuropathy. We therefore suggest naming this entity autosomal recessive cerebellar ataxia type 3 (ARCA3).
Comparison of 18F Fluoride PET/CT and 99mTc-MDP Bone Scan in the Detection of Skeletal Metastases in Urinary Bladder Carcinoma
Authors: Dhritiman Chakraborty, Anish Bhattacharya, Uttam Kumar Mete, Bhagwant Rai Mittal
Journal: Clinical Nuclear Medicine (2013)

The aim of this study was to compare (18)F-fluoride PET/CT and (99m)Tc-MDP bone scintigraphy in the detection of skeletal metastases in urinary bladder carcinoma.In this prospective study, 48 patients with urinary bladder carcinoma (44 male and 4 female patients, aged 35-80 years) underwent (99m)Tc-MDP planar and SPECT/CT bone scan (BS) followed by (18)F-fluoride PET/CT within 48 hours. Skeletal metastasis diagnosed on each of these techniques was compared against a final diagnosis based on contrast-enhanced CT, MRI, skeletal survey, clinical follow-up, and histological correlation.(18)F-fluoride PET/CT identified bony metastases and changed the management in 17 of 48 patients (35%). The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of (99m)Tc-MDP planar BS were 82.35%, 64.51%, 56%, 86.95%, and 70.83%; of (99m)Tc-MDP SPECT/CT were 88.23%, 74.19%, 65.21%, 92%, and 79.16%; and of (18)F-fluoride PET/CT were 100%, 87.09%, 80.95%, 100%, and 91.66%, respectively. Fair agreement between (99m)Tc-MDP planar BS and F-fluoride PET/CT (κ = 0.42) and excellent agreement between SPECT/CT and (18)F-fluoride PET/CT (κ = 0.74) were found.( 18)F-fluoride PET/CT has higher sensitivity, specificity, positive predictive value, negative predictive value, and accuracy in detecting bone metastases in urinary bladder carcinoma than conventional (99m)Tc-MDP planar BS. SPECT/CT improves all these parameters compared with planar BS and may serve as a cost-effective screening procedure for the detection of skeletal metastases in high-risk patients.
Goodbye. Introducing the new Chief Editors: Peter Sterk and Klaus Rabe
Authors: M. Decramer
Journal: European Respiratory Journal (2002)

At the end of this year my 3 yrs mandate as a Chief Editor expires. For a variety of reasons I have decided not to seek renewal of my mandate. As a consequence, I will be leaving the European Respiratory Journal ( ERJ ) and the European Respiratory Society (ERS) at the end of the year. It has been a great pleasure to lead this Journal into the new millennium and into an era of novelty. Indeed, in the past 3 yrs we were able to introduce novelty into the ERJ . There has been major novelty in the technical, logistical field as well as in the contents of the ERJ , which remain of the highest importance for a scientific journal.

In the logistical field we realised on-line distribution and on-line submission. We were the first respiratory journal to introduce a fully developed on-line submission system and were definitely the first journal to shift to full on-line submission at the beginning of this year. This has opened the way to on-line editing and publishing, which led to a complete reorganisation of the Sheffield office. We are indebted to Archie Turnbull, the ERS Executive Manager, Linda Arnold, the Publications Manager, and H. Charlesworth & Co. Ltd, our printers, who made all of this possible. The end result is that the distribution of the ERJ is now on time and that …
More methodological concerns
Authors: M. Ockendon, J. Gregory, G. Cribb, P. Cool
Journal: BMJ (2010)

We were surprised that only six out of 195 papers (or 20 infections) were considered suitable for inclusion in the meta-analysis of Smith and colleagues on sutures v staples for wound closure.1

The papers selected are a mixture of trauma and elective orthopaedics. These are two heterogeneous …
Comparison of outcome an average of 10 years after valve replacement with a mechanical versus a bioprosthetic valve. Results of the VA randomized trial
Authors: Karl E. Hammermeister, Gulshan K. Sethi, Charles Oprian, William G. Henderson, Edward Folland, Shukri Khuri, Shahbudin Rahimtoola
Journal: Journal of the American College of Cardiology (1991)
Oesophageal stricture and dysphagia after endoscopic sclerotherapy for bleeding varices.
Authors: T Sorensen, F Burcharth, M L Pedersen, F Findahl
Journal: Gut (1984)

Oesophageal stricture and dysphagia after endoscopic sclerotherapy of oesophageal varices were assessed with regard to occurrence and severity and the relation to the treatment. We followed 34 patients for three to 47 months who had two to 25 treatments with submucosal, paravenous injections of polidocanol (3%). Twenty patients (59%) developed stricture or dysphagia; 14 both dysphagia and endoscopically verified stricture, two dysphagia without stricture, and four stricture without dysphagia. Both phenomena occurred intermittently and often independent of each other, but occupied median 38% of the observation time in these 20 patients. The patients developing strictures had received significantly more treatments and greater amount of sclerosant, and they had significantly more preceding mucosal necroses. The varices were eradicated to about the same degree and the incidence of recurrent haemorrhage was the same as in the patients who had not developed stricture.
Mechanisms of Unstable Angina
Authors: Valentin Fuster, James H. Chesebro
Journal: New England Journal of Medicine (1986)

The hallmark of unstable angina is its unpredictability. The symptoms, which occur without provocation, sometimes stabilize or resolve but sometimes progress to myocardial infarction or sudden death. This pattern contrasts with that of stable angina.Early data suggested that transient increases in myocardial oxygen demand might explain episodes of pain that occurred at rest in patients with unstable angina.1 In the late 1970s, however, clinical, electrocardiographic, radionuclide, and myocardial bloodflow studies suggested that episodes of myocardial ischemia and pain at rest were the result of abrupt reductions in coronary blood flow. The emphasis had changed from the demand to the . . .
Diastolic filling dynamics in patients with aortic stenosis.
Authors: T Murakami, O M Hess, J E Gage, J Grimm, H P Krayenbuehl
Journal: Circulation (1986)

Left ventricular filling dynamics were investigated in 24 patients with aortic stenosis (AS). Biplane cineangiography was performed with simultaneous micromanometry in these 24 patients and in six control subjects. Twelve of the patients with AS had moderate hypertrophy with a left ventricular muscle mass index of less than 180 g/m2 (ASI group) and 12 had severe hypertrophy with an index of 180 g/m2 or more (AS2 group). Filling dynamics were also evaluated postoperatively in eight patients in the AS1 and six patients in the AS2 group. Preoperatively, end-diastolic and end-systolic volume indexes were larger and ejection fraction was lower in the AS2 compared with the control or AS1 group. Percent volume increase during the first half of diastole (%V1) was smaller in the AS1 than in the AS2 group. Peak filling rate in the first half of diastole (PFR 1) was higher in the AS2 than in the control or in AS1 group, while peak filling rate in the second half of diastole (PFR2) was considerably greater in the AS1 group than in the other two groups. The time constant of left ventricular pressure decline, an index of the rate of relaxation, was prolonged in the AS2 group. In contrast, mitral valve opening pressure (MVOP) was significantly higher in this group than in the other two groups. The constant of left ventricular chamber stiffness was slightly but not significantly greater in both AS groups than in the control subjects. After surgery in patients in the AS1 group, preoperatively reduced %V1 had increased and preoperatively enhanced PFR2 had decreased. In patients in the AS2 group, excluding one with a persistent low ejection fraction after surgery, preoperatively enhanced PFR1 decreased in association with a decrease in MVOP. Thus, left ventricular filling dynamics vary in patients with AS depending on the degree of left ventricular hypertrophy and systolic function. In patients with AS and moderate hypertrophy %V1 is slightly reduced but is compensated for by a forceful atrial contraction. In those with severe hypertrophy and systolic dysfunction increased driving pressure allows %V1 to remain within normal limits, despite prolonged left ventricular relaxation and decreased elastic recoil. Both changes in left ventricular filling dynamics tend to normalize after surgery in association with a reduction in left ventricular hypertrophy and/or an improvement of systolic function.