tbuckley/cabot-search huggingface dataset:
Substrates for Insulin-Receptor Kinase
Authors: Masato Kasuga, Tetsuro Izumi, Kazuyuki Tobe, Teruo Shiba, Kaoru Momomura, Yuko Tashiro-Hashimoto, Takashi Kadowaki
Journal: Diabetes Care (1990)

Several studies suggest that the tyrosine-specific protein kinase activity of the β-subunit of the insulin receptor is necessary to mediate the biological effects of insulin. This conclusion leads to the hypothesis that the effect of insulin is mediated through the tyrosine phosphorylation of cellular substrates by the insulin-receptor tyrosine kinase. In this review, the experimental evidence regarding insulin-stimulated phosphorylation of proteins both in vitro and in vivo is evaluated. In a cell-free system, tubulin, microtubuleassociated protein 2, tau, fodrin, calmodulin-dependent kinase, calmodulin, and lipocortins 1 and 2 were reported to be good substrates for insulin-receptor kinase. However, none were found to be tyrosine phosphorylated in an intact-cell system. In intact-cell systems, proteins of M, 185,000 (pp185), 120,000 (pp120), 240,000 (pp240), 15,000 (pp15), 60,000 (pp60), and 62,000 (pp62) as well as several others were reported to be tyrosine phosphorylated in an insulin-dependent fashion. However, the function or functional alteration of these proteins induced by insulin-stimulated tyrosine phosphorylation is not clear. Therefore, physiologically relevant substrates for the insulin-receptor kinase have not been established, and more work is necessary to verify the phosphorylation cascade hypothesis of insulin action.
Hazards of fibreoptic bronchoscopy.
Authors: P R Lanham
Journal: BMJ (1979)

patients was the thrombus confined to the calf.This is at variance with a large study,' in which thrombus was confined to the calf in 4900 of 493 legs containing thrombus.To say that "there was a general reluctance on the part of individual doctors to perform phlebography on patients with negative 99mTc-red-cell scans" merely emphasises the bias of investigation in favour of their own technique.Secondly, this technique apparently lacks the facility of radionuclide venography, its nearest rival in providing a simultaneous lung scan.Finally, the acid test of all new diagnostic procedures is whether they may be applied in the district general hospital as readily as the teaching hospital.Conventional phlebography fulfils this criterion.We remain unconvinced that this new technique can alter the situation in any way, being more demanding in time, expertise, skilled man- power, and expensive equipment.
Tumor necrosis factor-α stimulates fractalkine production by mesangial cells and regulates monocyte transmigration: Down-regulation by cAMP
Authors: Yung-Ming Chen, Shuei-Liong Lin, Ching-Wen Chen, Wen-Chih Chiang, Tun-Jun Tsai, Bor-Shen Hsieh
Journal: Kidney International (2003)
Safe Use of Latex Rubber
Authors: Gillian M. Shepherd
Journal: Annals of Internal Medicine (1995)

Letters1 August 1995Safe Use of Latex RubberGillian M. ShepherdGillian M. ShepherdAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-123-3-199508010-00014 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:In their recent review of latex allergy, Sussman and Beezhold [1] recommend that patients with a positive latex challenge use nonlatex gloves. This recommendation raises the question of the effectiveness of the substitutes—vinyl or synthetic latex (for example, neoprene). The Centers for Disease Control and Prevention and the Occupational Safety and Health Administration have stated that there are no significant differences in barrier protection between intact latex and vinyl gloves. During regular use, however, vinyl appears to tear more easily and is therefore substantially more permeable [2, 3]. Are the authors aware of data on the effectiveness ...References1. Sussman GL, Beezhold DH. Allergy to latex rubber. Ann Intern Med. 1995; 122:43-6. Google Scholar2. Korniewicz DM, Laughton BE, Cyr WH, et al. Leakage of virus through used vinyl and latex examination gloves. J Clin Microbiol. 1990; 28:787-8. Google Scholar3. DeGroot-Kosolcharoen J, Jones JM. Permeability of latex and vinyl gloves to water and blood. Am J Infect Control. 1989; 17:196-201. Google Scholar4. Yunginger JW, Jones RT, Fransway AF, Kelso JM, Warner MA, Hunt LW. Extractable latex allergens and proteins in disposable medical gloves and other rubber products. J Allergy Clin Immunol. 1994; 93:836-42. Google Scholar5. Swanson MC, Bubak ME, Hunt LE, Yunginger JW, Warner MA, Reed CE. Quantification of occupational latex aeroallergens in a medical center. J Allergy Clin Immunol. 1994; 94:445-51. Google Scholar Author, Article, and Disclosure InformationAuthors: Gillian M. Shepherd PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byBiocompatibilidade dos materiais em Ortodontia: mito ou realidade? 1 August 1995Volume 123, Issue 3Page: 234-235KeywordsAllergensAntigensAsthmaHealth care providersIntensive care unitsLatexPatientsPrevention, policy, and public healthProteinsResearch laboratories ePublished: 15 August 2000 Issue Published: 1 August 1995 Copyright & PermissionsCopyright © 1995 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Electrocardiographic Findings in Epidemic Hemorrhagic Fever
Authors: CARL M. PEARSON, ROBERT J. HALL
Journal: Circulation (1954)

Mounting experiences with the newly discovered disease, epidemic hemorrhagic fever indicate that the basic pathologic lesion affects the vascular system. An electrocardiographic survey in 53 cases has disclosed that during the acute phase of the illness more than 50 per cent of patients show alterations. These, however, are nonspecific and resemble changes recorded in other acute infections. There was no clinical or electrocardiographic evidence of residual cardiac injury in many cases re-evaluated up to six months after the acute illness.
Clinical and prognostic significance of in vivo differentiation in acute myeloid leukemia
Authors: Azra Raza, Harvey Preisler, Beatrice Lampkin, Joseph Lykins, Cathy Kukla, Peter Gartside, Yasin Sheikh, Naveed Yousuf, Michael White, Maurice Barcos, John Bennett, George Browman, Jack Goldberg, Hans Grunwald, Richard Larson, James Vardiman, Ralph Vogler
Journal: American Journal of Hematology (1993)

Abstract Bromodeoxyuridine (BrdU) was administered to 86 newly diagnosed patients with standard risk acute myeloid leukemia (AML) prior to starting induction therapy and the labeling index (LI), durations of S‐phase (Ts), and the cell cycle (Tc) of myeloblasts were determined. Induction therapy with cytosine arabinoside and daunomycin was subsequently started. Bone marrow biopsies were obtained on days 6 and 17 and weekly thereafter, and were treated with a monoclonal anti‐BrdU antibody to determine the fate of cells labeled on day 0 by BrdU. BrdU labeled granulocytes indicating the presence of in vivo differentiation (Diff+) were identified in 48 patients ranging from 1+ (1–10 labeled cells) to 4+ (greater than 31 labeled granulocytes). When compared to 38 differentiation negative (Diff‐) patients, Diff+ group had longer Ts (14.5 hr vs. 10.95 hr, P = 0.015) and Tc (59.7 hr vs. 41.7 hr, P = 0.017). Remission duration was significantly longer (no median) for 3–4+ Diff+ as compared to Diff‐ (median = 220 days) patients (Wilcoxon P = 0.04). We conclude that the detection of in vivo differentiation in AML patients indicates a favorable long‐term prognosis either due to the presence of a substantial amount of normal residual hematopoiesis prior to starting induction therapy or due to the ability of leukemic cells to undergo differentiation. © 1993 Wiley‐Liss, Inc.
Absorption Kinetics of Semisynthetic Human Insulin and Biosynthetic (recombinant DNA)Human Insulin
Authors: F W Kemmer, G Sonnenberg, H J Cüppers, M Berger
Journal: Diabetes Care (1982)

Absorption kinetics of subcutaneously injected human insulin (recombinant DNA) and semisynthetic human regular insulin were investigated and compared to the respective porcine insulin preparations in normal volunteers. The absorption of all regular human insulin preparations tested was accelerated as compared to porcine insulins. The clinical relevance of these findings in regard to the treatment of diabetes mellitus appears to be doubtful. As for mixtures of porcine regular and intermediate-acting insulin preparations, the absorption of human regular insulin is not altered when mixed with intermediate-acting human insulin.
Insulin delivery rate into plasma in normal and diabetic subjects
Authors: Michael P. Stern, John W. Farquhar, Abraham Silvers, Gerald M. Reaven
Journal: Journal of Clinical Investigation (1968)

Removal of insulin-(131)I from plasma was studied in normal and diabetic subjects with both single injection and continuous infusion of isotope techniques. Patients were studied either in the fasting state or during steady-state hyperglycemia produced by a continuous intravenous glucose infusion. Steady-state plasma insulin concentration during these studies ranged from 10 to 264 muU/ml. Labeled insulin specific activity time curves consisted of more than one exponential, indicating that a multicompartmental system for insulin metabolism exists. A mathematical technique which is applicable to non-first order processes was used to calculate the rate at which insulin was lost irreversibly from the plasma insulin pool. A direct, linear relationship was found between insulin irreversible loss rate and plasma insulin concentration over the range of concentrations studied. This linearity implies lack of saturability of the insulin removal mechanism. Since the plasma insulin pool was in a steady state during these studies, insulin irreversible loss rate was equal to the rate at which newly secreted insulin was being delivered to the general circulation. Therefore, these results indicate that changes in plasma insulin concentration result from parallel changes in the rate of insulin delivery and not from changes in the opposite direction of the rate of insulin removal. A wide range of insulin delivery rates was found among patients with similar plasma glucose concentrations, suggesting that there exists considerable variability in responsiveness to endogenous insulin among these patients.
Leukoreduction vs Buffy-Coat Depletion and the Safety of Blood Transfusion
Authors: J. P. Wallis
Journal: JAMA (2003)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Determination of 20(S)-protopanaxadiol in human plasma by HPLC–MS/MS: application to a pharmacokinetic study
Authors: Feifan Xie, Sanwang Li, Zeneng Cheng, Xingling Liu, Hong Zhang, Peijiong Li, Zhi Liu, Xin Guo, Peng Yu
Journal: Acta Pharmaceutica Sinica B (2013)

A rapid, specific and sensitive HPLC–MS/MS method was developed and validated for the determination of 20(S)-protopanaxadiol (PPD) in human plasma. PPD and the internal standard PD were extracted from plasma by liquid–liquid extraction with cyclohexane–methylene dichloride (2:1, v/v). The separation was performed on a HyPURIYTY C18 column using methanol–5 mM ammonium formate (90:10, v/v) as mobile phase at a flow rate of 0.35 mL/min. Mass spectrometric detection was carried out by electrospray ionization (ESI) in the positive ion mode using multiple reaction monitoring (MRM). The monitored transitions were m/z 425.4→217.2 for PPD and at m/z 461.4→425.5 for PD. The method was linear over the range 0.512–100 ng/mL with a lower limit of quantification (LLOQ) of 0.512 ng/mL. The mean extraction recovery of PPD was greater than 78.2% and no significant matrix effect was detected. The intra- and inter-day precisions were less than 10% and the biases below 4% for PPD. The validated method was applied to a three-level single-dose clinical pharmacokinetics study of 12 healthy Chinese volunteers and the main pharmacokinetic parameters of PPD were obtained.
Low-Level Gestational Lead Exposure Increases Retinal Progenitor Cell Proliferation and Rod Photoreceptor and Bipolar Cell Neurogenesis in Mice
Authors: Anand Giddabasappa, W. Ryan Hamilton, Shawntay Chaney, Weimin Xiao, Jerry E. Johnson, Shradha Mukherjee, Donald A. Fox
Journal: Environmental Health Perspectives (2010)

BackgroundGestational lead exposure (GLE) produces novel and persistent rod-mediated electroretinographic (ERG) supernormality in children and adult animals.ObjectivesWe used our murine GLE model to test the hypothesis that GLE increases the number of neurons in the rod signaling pathway and to determine the cellular mechanisms underlying the phenotype.ResultsBlood lead concentrations ([BPb]) in controls and after low-, moderate-, and high-dose GLE were ≤ 1, ≤ 10, approximately 25, and approximately 40 μg/dL, respectively, at the end of exposure [postnatal day 10 (PND10)]; by PND30 all [BPb] measures were ≤ 1 μg/dL. Epifluorescent, light, and confocal microscopy studies and Western blots demonstrated that late-born rod photoreceptors and rod and cone bipolar cells (BCs), but not Müller glial cells, increased in a nonmonotonic manner by 16–30% in PND60 GLE offspring. Retinal lamination and the rod:cone BC ratio were not altered. In vivo BrdU (5-bromo-2-deoxyuridine) pulse-labeling and Ki67 labeling of isolated cells from developing mice showed that GLE increased and prolonged retinal progenitor cell proliferation. TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) and confocal studies revealed that GLE did not alter developmental apoptosis or produce retinal injury. BrdU birth-dating and confocal studies confirmed the selective rod and BC increases and showed that the patterns of neurogenesis and gliogenesis were unaltered by GLE.ConclusionsOur findings suggest two spatiotemporal components mediated by dysregulation of different extrinsic/intrinsic factors: increased and prolonged cell proliferation and increased neuronal (but not glial) cell fate. These findings have relevance for neurotoxicology, pediatrics, public health, risk assessment, and retinal cell biology because they occurred at clinically relevant [BPb] and correspond with the ERG phenotype.
Targeting 4-1BB Costimulation to Disseminated Tumor Lesions With Bi-specific Oligonucleotide Aptamers
Authors: Fernando Pastor, Despina Kolonias, James O McNamara II, Eli Gilboa
Journal: Molecular Therapy (2011)

The paucity of costimulation at the tumor site compromises the ability of tumor-specific T cells to eliminate the tumor. Here, we show that bi-specific oligonucleotide aptamer conjugates can deliver costimulatory ligands to tumor cells in situ and enhance antitumor immunity. In poorly immunogenic subcutaneously implanted tumor and lung metastasis models, systemic delivery of an agonistic 4-1BB aptamer ligand conjugated to a prostate specific membrane antigen (PSMA)-binding tumor-targeting aptamer led to inhibition of tumor growth, was more effective than, and synergized with, vaccination, and exhibited a superior therapeutic index compared to costimulation with 4-1BB antibodies. Tumor inhibition was dependent on homing to PSMA-expressing tumor cells and 4-1BB costimulation. Aptamer targeted costimulation is a broadly applicable and clinically feasible approach to enhance the costimulatory environment of disseminated tumor lesions. This study suggests that potentiating naturally occurring antitumor immunity via tumor-targeted costimulation could be an effective approach to elicit protective immunity to control tumor progression in cancer patients.
Pseudohypoxemia Secondary to Leukemia and Thrombocytosis
Authors: Charles E. Hess, Allen B. Nichols, William B. Hunt, Paul M. Suratt
Journal: New England Journal of Medicine (1979)

IN the interval between drawing of arterial blood and analysis of blood-gas composition, partial pressure of oxygen (PO2) and pH fall, and the partial pressure of carbon dioxide (PCO2) rises; the rate of these changes is dependent on the temperature.1 , 2 In addition, the rate of fall in partial pressure of oxygen in arterial blood (PaO2) depends on the initial level of PaO2 and also roughly correlates with the white-cell count.2 3 4 There are no data, however, concerning the effect of the specific cellular composition of blood on values of arterial blood gas. This investigation . . .
Fetal PCB syndrome: clinical features, intrauterine growth retardation and possible alteration in calcium metabolism.
Authors: F Yamashita, M Hayashi
Journal: Environmental Health Perspectives (1985)

Pregnant mothers with yusho in Fukuoka, Nagasaki and Kochi Prefectures delivered babies with a peculiar clinical manifestation which will be called fetal PCB syndrome (FPS). The birth rate incidences were 3.6% (Fukuoka Prefecture), 4% (Nagasaki Prefecture), 2.9% (Kochi Prefecture) and 3.9% (total). The manifestations consisted of dark brown pigmentation of the skin and the mucous membrane, gingival hyperplasia, exophthalmic edematous eye, dentition at birth, abnormal calcification of the skull as demonstrated by X-ray, rocker bottom heel and high incidence of light for date (low birth weight) babies. We suggest that there may be a possible alteration in calcium metabolism in these babies, related to the fragile egg shells observed in PCB-contaminated birds and to the female hormone-enhancing effect of PCB. The high incidence of low birth weight among these newborns and two other similar studies indicated that PCBs suppress fetal growth.
Gene transfer into the airway epithelium of animals by targeting the polymeric immunoglobulin receptor.
Authors: T Ferkol, J C Perales, E Eckman, C S Kaetzel, R W Hanson, P B Davis
Journal: Journal of Clinical Investigation (1995)

Genes of interest can be targeted specifically to respiratory epithelial cells in intact animals with high efficiency by exploiting the receptor-mediated endocytosis of the polymeric immunoglobulin receptor. A DNA carrier, consisting of the Fab portion of polyclonal antibodies raised against rat secretory component covalently linked to poly-L-lysine, was used to introduce plasmids containing different reporter genes into airway epithelial cells in vivo. We observed significant levels of luciferase enzyme activity in protein extracts from the liver and lung, achieving maximum values of 13,795 +/- 4,431 and 346,954 +/- 199,120 integrated light units (ILU) per milligram of protein extract, respectively. No luciferase activity was detected in spleen or heart, which do not express the receptor. Transfections using complexes consisting of an irrelevant plasmid (pCMV lacZ) bound to the bona fide carrier or the expression plasmid (pGEMluc) bound to a carrier based on an irrelevant Fab fragment resulted in background levels of luciferase activity in all tissues examined. Thus, only tissues that contain cells bearing the polymeric immunoglobulin receptor are transfected, and transfection cannot be attributed to the nonspecific uptake of an irrelevant carrier-DNA complex. Specific mRNA from the luciferase gene was also detected in the lungs of transfected animals. To determine which cells in the lungs are transfected by this method, DNA complexes were prepared containing expression plasmids with genes encoding the bacterial beta-galactosidase or the human interleukin 2 receptor. Expression of these genes was localized to the surface epithelium of the airways and the submucosal glands, and not the bronchioles and alveoli. Receptor-mediated endocytosis can be used to introduce functional genes into the respiratory epithelium of rats, and may be a useful technique for gene therapy targeting the lung.
The Effects of Cycle Length on Cardiac Refractory Periods in Man
Authors: PABLO DENES, DELON WU, RAMESH DHINGRA, RAYMOND J. PIETRAS, KENNETH M. ROSEN
Journal: Circulation (1974)

The effects of pacing-induced changes in cycle length on the refractory periods of the atrium, A-V node and His-Purkinje system were studied in 24 patients using the extra stimulus technique. Refractory period determinations were made at two or more cycle lengths in all patients. Slopes relating cycle length and refractory periods were calculated using the least squares method. Both the effective and functional refractory periods (ERP and FRP) of the atrium shortened with decreasing cycle lengths, with a mean slope of +0.155 and +0.129 respectively. A-V nodal ERP lengthened (mean slope, –0.177) while A-V nodal FRP shortened slightly (mean slope, +0.126). Bundle branch refractory periods as well as relative refractory periods of the His-Purkinje system also decreased, with mean slopes of +0.270 and +0.360, respectively. The ERP of the A-V node at any cycle length was related to the A-H at that cycle length ( r = +0.646, P &lt; 0.001). The responses of the human heart to changes in cycle length are generally similar to those previously described in the animal laboratory. Such information contributes to our understanding of electrocardiographic phenomena such as aberrant conduction.
WHAT IS THE BEST METHOD TO ASSESS MITRAL VALVULAR OBSTRUCTION AFTER MITRAL ANNULOPLASTY?
Authors: Heping Deng, Dalane Kitzman, Gregory Hundley, John Hoyle, Gretchen Wells, Pamela Burgess, Takahiro Ohara, Meng Meng, Neal Kon, Min Pu
Journal: Journal of the American College of Cardiology (2011)
The regeneration of transected sciatic nerves of adult rats using chitosan nerve conduits seeded with bone marrow stromal cell-derived Schwann cells
Authors: Qiang Ao, Chun-Kit Fung, Alex Yat-Ping Tsui, Sa Cai, Huan-Cong Zuo, Ying-Shing Chan, Daisy Kwok-Yan Shum
Journal: Biomaterials (2010)
Pneumonitis Due to<i>Cryptostroma corticale</i>(Maple-Bark Disease)
Authors: Dean A. Emanuel, Frederick J. Wenzel, Ben R. Lawton
Journal: New England Journal of Medicine (1966)

MAPLE-bark disease is an unusual disorder that affects both trees and man. The fungus, Cryptostroma corticale (Fig. 1), can cause death to the sycamore tree1 and also a totally disabling pulmonary disease in man.2 , 3 There are only 2 reports in the literature of its effect on man, the first by Towey, Sweany and Huron2 (1932) and the second by Emanuel, Lawton and Wenzel3 (1962).Since 1961 we have seen 5 patients in whom the diagnosis has been verified by pulmonary biopsy (Table 1). This report contains the clinical and laboratory studies of the men in whom clinical disease developed after . . .
Predictive value of oxygen saturation in emergency evaluation of asthmatic children.
Authors: G. C. Geelhoed, L. I. Landau, P. N. LeSouef
Journal: BMJ (1988)

Etude chez 52 enfants atteints d'asthme aigu traites en service d'urgence. La saturation d'oxygene du sang arteriel au depart est un meilleur indice de pronostic que la reponse au traitement. Les enfants dont la saturation d'oxygene est faible au debut devraient etre hospitalises meme si les premieres reponses au traitement semblent bonnes