tbuckley/cabot-search huggingface dataset:
Abstract 5672: Mibefradil increases paclitaxel's effect on MCF-7 cell proliferation <i>in vitro</i> and <i>in vivo</i>
Authors: Jonathan Pottle, Chengrong Sun, Lloyd Gray, Ming Li
Journal: Cancer Research (2012)

Abstract T-type Ca2+ channel mediated Ca2+ influx is important for tumor cell proliferation. In order to understand the role of this influx in tumor cell cycling, we conducted experiments to block T-type calcium channels in MCF7 breast cancer cells using pharmacological inhibitors, mibefradil or its more T-type calcium channel specific derivative NNC-55-0396, and examined the effects of this blockade on the passage through the cell cycle of MCF7 cells and whether T-type calcium channel inhibition can be used in conjunction with paclitaxel chemotherapy to increase the efficacy of the chemotherapy. Flow cytometry shows that blocking T-type calcium channels with the specific inhibitor NNC-55-0396 resulted in an increase in MCF7 cells in the G1 phase and a decrease in cells in the S phase of the cell cycle. Treatment with paclitaxel for 72 hours in vitro yielded lower MCF7 cell numbers than treatment with NNC-55-0396, as measured by a cell proliferation assay. However, a treatment regimen alternating NNC-55-0396 with paclitaxel over 72 hours was found to reduce MCF7 cell number to a larger degree than either agent alone for the same time period. For in vivo modeling, MCF7 xenografts were implanted in the mammary fat pads of nude mice, with tumor size monitored via caliper measurements. Similar to the in vitro findings, a treatment regiment that alternated paclitaxel chemotherapy with the calcium channel inhibitor mibefradil reduced tumor size to a larger degree than either agent alone, though that difference did not rise to a statistically significant level. These results indicate that blocking T-type calcium channels halts proliferating MCF7 cells at the G1/S checkpoint, and that this phenomenon may lead to an increased sensitivity to paclitaxel chemotherapy. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5672. doi:1538-7445.AM2012-5672
Assessment of a Bronchoscopy Simulator
Authors: DAVID OST, ANDREW DE ROSIERS, E. JAMES BRITT, ALAN M. FEIN, MARTIN L. LESSER, ATUL C. MEHTA
Journal: American Journal of Respiratory and Critical Care Medicine (2001)

The study objective was to validate a flexible bronchoscopy simulator by determining if it could differentiate between expert and novice bronchoscopists. A subsequent evaluation phase was then done to determine whether use of the simulator would improve the rate of bronchoscopy skill acquisition for new pulmonary fellows. A multicenter prospective cohort study was performed using a bronchoscopy simulator. Three cohorts were evaluated based on the number of bronchoscopies previously performed: "experts" ( > 500, n = 9), "intermediates" (25 to 500, n = 8), and "novices" (none, n = 11). Each participant performed two simulated cases with performance measures being recorded by the simulator. Performance measures that distinguished between groups were then used to evaluate the learning curve for new fellows training on the simulator. A randomized-controlled trial was then conducted comparing the quality of bronchoscopy performance for new pulmonary fellows who were trained either with conventional methods or with the simulator. Expert bronchoscopists performed better on the simulator than intermediates who performed better than novices in terms of procedure time, percentage of segments visualized, time in red-out, and wall collisions. Training of new fellows demonstrated that after performing 20 bronchoscopic simulations, the skill level acquired with the simulator significantly improved in terms of speed, percentage of segments visualized, time in red-out, and collisions. Fellows trained on the simulator performed better than fellows trained using conventional methods during their first actual bronchoscopies as assessed by procedure time (815 versus 1,168 s, p = 0.001), a bronchoscopy nurse's subjective quality assessment score (7.7 ± 0.3 versus 3.7 ± 2.5, p = 0.05), and by a quantitative bronchoscopy quality score (percentage of segments correctly identified/procedure time, 0.119 ± 0.015 versus 0.046 ± 034, p = 0.03). In conclusion, the bronchoscopy simulator was able to accurately assess bronchoscopy experience level. Training new fellows on the bronchoscopy simulator leads to more rapid acquisition of bronchoscopy expertise compared with conventional training methods. This technology has the potential to facilitate bronchoscopy training and to improve objective evaluations of bronchoscopy skills.
Treatment of Craniocerebral Injuries
Authors: Edward L. Spatz
Journal: New England Journal of Medicine (1961)

NEWSPAPERS daily accent the magnitude of the problem of craniocerebral trauma. In the twenty years from 1934 to 1954 more than 700,000 persons were killed in automobile crashes. In 1953 deaths from poliomyelitis numbered less than 2000, whereas automobile fatalities numbered some 38,000.1 By a conservative estimate 50 to 70 per cent of these deaths were from head injury.It is the purpose of this paper to present the problem of craniocerebral trauma in broad perspective. It is hoped that this will afford a frame of reference in which progress in treatment may be considered. Of necessity, many areas will . . .
Is ε-Aminocaproic Acid as Effective as Aprotinin in Reducing Bleeding With Cardiac Surgery?
Authors: John J. Munoz, Nancy J. O. Birkmeyer, John D. Birkmeyer, Gerald T. O’Connor, Lawrence J. Dacey
Journal: Circulation (1999)

Although aprotinin is known to be effective in reducing postoperative hemorrhage after cardiac surgery, epsilon-aminocaproic acid, an alternative antifibrinolytic, is considerably less expensive. Because the results of 3 small randomized clinical trials comparing these 2 agents directly were inconclusive, we performed a meta-analysis to compare the relative effectiveness and adverse-effect profile of these 2 agents against placebo.Data from 52 randomized clinical trials published between 1985 and 1998 involving the use of epsilon-aminocaproic acid (n=9) or aprotinin (n=46) in patients undergoing cardiac surgery were abstracted. Our primary outcomes were total blood loss, red blood cell transfusion rates and amounts, reexploration, stroke, myocardial infarction, and mortality. The meta-analysis revealed substantial reductions in total blood loss with epsilon-aminocaproic acid and low-dose aprotinin (each with a 35% reduction versus placebo, P<0.001) and high-dose aprotinin (53% reduction, P<0.001). There were identical reductions in total postoperative transfusions with epsilon-aminocaproic acid (61% reduction versus placebo, P<0. 010) and high-dose aprotinin (62% reduction, P<0.001). The proportion of patients transfused was similarly reduced with epsilon-aminocaproic acid (OR, 0.32; 95% CI, 0.15 to 0.69) and high-dose aprotinin (OR, 0.28; 0.22 to 0.37). Although both drugs reduced rates of reexploration to similar degrees, this effect was statistically significant only with high-dose aprotinin (OR, 0.39; 0. 24 to 0.61). epsilon-Aminocaproic acid and aprotinin had no effect on risks of postoperative myocardial infarction or overall mortality.Because the 2 antifibrinolytic agents appear to have similar efficacies, the considerably less-expensive epsilon-aminocaproic acid may be preferred over aprotinin for reducing hemorrhage with cardiac surgery.
Comment on: Leeds et al. High Prevalence of Microvascular Complications in Adults With Type 1 Diabetes and Newly Diagnosed Celiac Disease. Diabetes Care 2011;34:2158–2163
Authors: Noemi Malandrino, Esmeralda Capristo
Journal: Diabetes Care (2012)

We read with interest the article by Leeds et al. (1) describing the prevalence of increased microvascular complications in patients with type 1 diabetes and newly diagnosed celiac disease (CD).

Leeds et al. stated that adults with type 1 diabetes and undetected CD have worse glycemic control with more renal disease and more retinopathy compared with patients with type 1 diabetes alone. The newly diagnosed CD patients in the cohort of type 1 diabetes patients are matched for age, duration of type 1 …
Changes in Quality of Life Through the Intervention by a Palliative Care Team for Patients with Advanced Lung Cancer
Authors: T. Yokoyama, H. Kunikane, H. Okamoto, N. Hida, T. Shimokawa, A. Sato, Y. Misumi, Y. Agemi, M. Ishii, H. Nishibeppu, Y. Fukushima, R. Ooishi
Journal: Annals of Oncology (2013)
IMPACT OF MITRAL ANNULUS DIMENSIONS ON EFFECTIVENESS OF PERCUTANEOUS EDGE-TO-EDGE MITRAL VALVE REPAIR AND PREDICTION OF LEFT ATRIAL AND LEFT VENTRICULAR REVERSED REMODELING: ANALYSIS BY 2D AND 3D ECHOCARDIOGRAPHY
Authors: Ertunc Altiok, Michael Becker, Christian Zwicker, Sandra Hamada, Nikolaus Marx, Rainer Hoffmann
Journal: Journal of the American College of Cardiology (2012)
Spectrum of antibiotic-associated diarrhoea.
Authors: A H Lishman, I J Al-Jumaili, C O Record
Journal: Gut (1981)

In an attempt to find the extent to which Clostridium difficile could be implicated as the cause of antibiotic-associated diarrhoea, the stools of 53 patients who had diarrhoea after a course of antibiotics were investigated for the presence of C. difficile toxin. Ten of the patients (19%) were found to be positive, but the stools of four out of 53 patients without diarrhoea after a course of antibiotics were also found to contain C. difficile toxin (7.5%). The titre of toxin in patients both with and without diarrhoea fell within the same range (up to 10(-5)). Neither the organism nor its toxin was found in the stool of 26 patients with ulcerative colitis, eight with Crohn's disease, 49 with non-specific diarrhoea, and 27 normal controls. We conclude that, while C. difficile is responsible for a proportion of cases of antibiotic-associated diarrhoea, the concentration of toxin is not the sole factor affecting the severity of this disorder.
The missing link's missing link: Syllabic vocalizations at 3 months of age
Authors: Kathleen Bloom
Journal: Behavioral and Brain Sciences (1998)

If syllables are the link between nonhuman calls and human speech, as MacNeilage suggests, then that link is actually revealed in the “syllabic” sounds of the 3-month-old infant, well before the reduplicative babbling of the 8-month-old. Anatomical, acoustic, cognitive, and social perceptual evidence supports this earlier landmark.
Obituary Listing
Authors: Gregory A. Hood
Journal: JAMA (1998)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
The neural substrates of recollection and familiarity
Authors: Andrew P. Yonelinas, Neal E. A. Kroll, Ian G. Dobbins, Michele Lazzara, Robert T. Knight
Journal: Behavioral and Brain Sciences (1999)

Aggleton &amp; Brown argue that a hippocampal-anterior thalamic system supports the “recollection” of contextual information about previous events, and that a separate perirhinal-medial dorsal thalamic system supports detection of stimulus “familiarity.” Although there is a growing body of human literature that is in agreement with these claims, when recollection and familiarity have been examined in amnesics using the process dissociation or the remember/know procedures, the results do not seem to provide consistent support. We reexamine these studies and describe the results of an additional experiment using a receiver operating characteristic (ROC) technique. The results of the reanalysis and the ROC experiment are consistent with Aggleton &amp; Brown's proposal. Patients with damage to both regions exhibit severe deficits in recollection and smaller, but consistent, deficits in familiarity.
Hypovolemia in the Nephrotic Syndrome — A Contraindication to Renal Biopsy
Authors: Hiroshi Yamauchi, James Hopper, Kenneth McCormack, Katherine Lambert
Journal: New England Journal of Medicine (1960)

TO date, we have performed a total of 150 percutaneous renal biopsies in patients with various types of kidney disease, including the nephrotic syndrome. The 1 fatal case in our series occurred in 1957, when a young woman with systemic lupus erythematosus and the nephrotic syndrome died shortly after the biopsy procedure. Although blood-volume measurements were not done in this case, a review of the clinical course, together with the findings at autopsy, led us to believe that hypovolemia had been present and was a contributory factor in the patient's death. Since that time we have routinely performed blood-volume measurements . . .
Positron Emission Tomography Imaging of Atherosclerosis
Authors: Hakan Orbay, Hao Hong, Yin Zhang, Weibo Cai
Journal: Theranostics (2013)

Atherosclerosis-related cardiovascular events are the leading causes of death in the industrialized world.Atherosclerosis develops insidiously and the initial manifestation is usually sudden cardiac death, stroke, or myocardial infarction.Molecular imaging is a valuable tool to identify the disease at an early stage before fatal manifestations occur.Among the various molecular imaging techniques, this review mainly focuses on positron emission tomography (PET) imaging of atherosclerosis.The targets and pathways that have been investigated to date for PET imaging of atherosclerosis include: glycolysis, cell membrane metabolism (phosphatidylcholine synthesis), integrin α v β 3, low density lipoprotein (LDL) receptors (LDLr), natriuretic peptide clearance receptors (NPCRs), fatty acid synthesis, vascular cell adhesion molecule-1 (VCAM-1), macrophages, platelets, etc.Many PET tracers have been investigated clinically for imaging of atherosclerosis.Early diagnosis of atherosclerotic lesions by PET imaging can help to prevent the premature death caused by atherosclerosis, and smooth translation of promising PET tracers into the clinic is critical to the benefit of patients.
Systemic Blastomycosis
Authors: Francis J. Marx, Arthur A. Berenbaum
Journal: New England Journal of Medicine (1954)

THE occurrence of a case of an infectious disease in a geographic location outside an area recognized as endemic is always of clinical interest. Diseases hitherto associated with arbitrary geographic boundaries must now be considered more frequently in the differential diagnosis of obscure infections.1 , 2 The purpose of this presentation is to report a case of systemic blastomycosis in Philadelphia and to demonstrate some of the unusual features concerning the difficulties in diagnosis and management, the rapidity of the clinical course and the failure of response to specific therapy.Case ReportJ.F., an 18-year-old Negro, was admitted to the St. Luke's . . .
AP2 adaptor complex mediates bile salt export pump internalization and modulates its hepatocanalicular expression and transport function
Authors: Hisamitsu Hayashi, Kaori Inamura, Kensuke Aida, Sotaro Naoi, Reiko Horikawa, Hironori Nagasaka, Tomozumi Takatani, Tamio Fukushima, Asami Hattori, Takashi Yabuki, Ikuo Horii, Yuichi Sugiyama
Journal: Hepatology (2012)

The bile salt export pump (BSEP) mediates the biliary excretion of bile salts and its dysfunction induces intrahepatic cholestasis. Reduced canalicular expression of BSEP resulting from the promotion of its internalization is one of the causes of this disease state. However, the molecular mechanism underlying BSEP internalization from the canalicular membrane (CM) remains unknown. We have shown previously that 4-phenylbutyrate (4PBA), a drug used for ornithine transcarbamylase deficiency (OTCD), inhibited internalization and subsequent degradation of cell-surface-resident BSEP. The current study found that 4PBA treatment decreased significantly the expression of α- and μ2-adaptin, both of which are subunits of the AP2 adaptor complex (AP2) that mediates clathrin-dependent endocytosis, in liver specimens from rats and patients with OTCD, and that BSEP has potential AP2 recognition motifs in its cytosolic region. Based on this, the role of AP2 in BSEP internalization was explored further. In vitro analysis with 3×FLAG-human BSEP-expressing HeLa cells and human sandwich-culture hepatocytes indicates that the impairment of AP2 function by RNA interference targeting of α-adaptin inhibits BSEP internalization from the plasma membrane and increases its cell-surface expression and transport function. Studies using immunostaining, coimmunoprecipitation, glutathione S - transferase pulldown assay, and time-lapse imaging show that AP2 interacts with BSEP at the CM through a tyrosine motif at the carboxyl terminus of BSEP and mediates BSEP internalization from the CM of hepatocytes. Conclusion: AP2 mediates the internalization and subsequent degradation of CM-resident BSEP through direct interaction with BSEP and thereby modulates the canalicular expression and transport function of BSEP. This information should be useful for understanding the pathogenesis of severe liver diseases associated with intrahepatic cholestasis.
Autoimmune hepatitis (AIH): clinical spectrum of disease defined by revised international autoimmune hepatitis group (IAHG) scoring system
Authors: Anna Baragiotta, Oliver F.W. James, Wendy Craig, Harriet Mitchison, Denis Burke, Malcolm Bateson, Peter Trewby, Antony Macklon, Margaret F. Bassendine
Journal: Journal of Hepatology (2002)
Metabolic Abnormalities Associated with Intentional Theophylline Overdose
Authors: KEVIN W. HALL
Journal: Annals of Internal Medicine (1984)

To verify the frequency and severity of metabolic abnormalities occurring as a result of intentional theophylline overdose, we retrospectively analyzed admission laboratory data from 22 cases of theophylline poisoning. Hypokalemia, hyperglycemia, and leukocytosis were frequent findings, and the magnitude of these abnormalities was related to the corresponding serum theophylline level. Although measurements were available for a smaller number of patients, respiratory alkalosis, hypophosphatemia, and hypomagnesemia were also common findings. Given the potential contribution of these abnormalities to the cardiovascular and neurologic toxicities of theophylline, it is important that their presence in patients with overdose be anticipated.
Radioiodine for Graves Disease
Authors: DANIEL GLINOER
Journal: Annals of Internal Medicine (1987)

Letters and Corrections1 April 1987Radioiodine for Graves DiseaseDANIEL GLINOER, M.D., Ph.D.DANIEL GLINOER, M.D., Ph.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-106-4-637_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: The recent review by Graham and Burman (1) proposes greater use of radioiodine as primary treatment in Graves disease. Similar views have recently been proposed by Hennemann and associates (2). The proposal made by these authors to extend the indications for radioiodine treatment is, however, contradicted to a large extent by the facts.In 1986, members of the European Thyroid Association were invited to participate in a survey on the management of hyperthyroidism due to Graves disease (3). The aim of the survey was to assess the use of diagnostic procedures and therapeutic practices in European centers...References1. GRAHAMBURMAN GK. Radioiodine treatment of Graves' disease. Ann Intern Med. 1986;105:900-5. LinkGoogle Scholar2. HENNEMANNKRENNINGSANKARANARAYANAN GEK. Place of radioactive iodine in treatment of thyrotoxicosis. Lancet. 1986;1:1369-72. CrossrefMedlineGoogle Scholar3. GLINOERHESCHLAGASSELAURBERG DRRP. The management of hyperthyroidism due to Graves' disease: results of an international survey by the European Thyroid Association. Acta Endocrinol. 1987. (In press). Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: DANIEL GLINOER, M.D., Ph.D.Affiliations: University Hospital St-Pierre Brussels Belgium PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 April 1987Volume 106, Issue 4Page: 637-637KeywordsHyperthyroidismThyroid ePublished: 1 December 2008 Issue Published: 1 April 1987 PDF downloadLoading ...
Liquid Marbles for High‐Throughput Biological Screening of Anchorage‐Dependent Cells
Authors: Nuno M. Oliveira, Clara R. Correia, Rui L. Reis, João F. Mano
Journal: Advanced Healthcare Materials (2014)

Stable liquid marbles (LM) are produced by coating liquid droplets with a hydrophobic powder. The used hydrophobic powder is produced by fluorosi­lanization of diatomaceous earth, used before to produce superhydrophobic structures. Here, the use of LM is proposed for high‐throughput drug screening on anchorage‐dependent cells. To provide the required cell adhesion sites inside the liquid environment of LM, surface‐modified poly( l ‐lactic acid) microparticles are used. A simple method that takes advantage from LM appealing features is presented, such as the ability to inject liquid on LM without disrupting (self‐healing ability), and to monitor color changes inside of LM. After promoting cell adhesion, a cytotoxic screening test is performed as a proof of concept. Fe 3+ is used as a model cytotoxic agent and is injected on LM. After incubation, AlamarBlue reagent is injected and used to assess the presence of viable cells, by monitoring color change from blue to red. Color intensity is measured by image processing and the analysis of pictures takes using an ordinary digital camera. The proposed method is fully validated in counterpoint to an MTS (3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carbo​xymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐te​trazolium) colorimetric assay, a well‐known method used for the cytotoxicity assessment.
Generalizing Lung-Cancer Screening Results
Authors: Marlies E. Heuvers, Bruno H. Stricker, Joachim G. Aerts
Journal: New England Journal of Medicine (2012)

Investigators in the Rotterdam Study note that only about 30% of their study population met the entry criteria for the National Lung Screening Trial (NLST). Others at some risk were excluded; whether the 20% reduction in mortality seen in the NLST will transfer to other risk groups is unclear.
Aspirin can continue to reduce blood clot risk after anticoagulants for venous thromboembolism, study finds
Authors: I. Torjesen
Journal: BMJ (2014)

Aspirin should be considered as a follow-on treatment in patients who have finished a course of treatment with a vitamin K antagonist or a newer anticoagulant after venous thromboembolism, to continue to reduce their risk of further blood clots, research published in Circulation has found.1

Patients who have venous thromboembolism with no obvious cause have an average 10% risk of another clot within one year and a 5% risk each year thereafter. After the event patients usually receive warfarin or a newer anticoagulant for six to 12 months …