tbuckley/cabot-search huggingface dataset:
Abstract 2256: PDEF regulation and role as a survival factor in ER positive breast cancer cells
Authors: Gilles Buchwalter, Myles Brown
Journal: Cancer Research (2012)

Abstract PDEF (Prostate Derived Ets Factor) is a member of the ‘Ets’ family. These transcription factors regulate a number of biological processes, including cell proliferation, differentiation, invasion and are thought to play an important role in oncogenesis. PDEF is expressed exclusively in tissues with a high epithelial content, especially hormone-regulated epithelium, such as prostate, mammary gland, endometrium and ovary. Furthermore, several studies showed PDEF to be one of the most highly over expressed mRNA in human and mouse mammary tumors. PDEF is interestingly significantly co-expressed with ER in ER positive breast carcinomas, together with known ER cofactors such as FOXA1, XBP1 and GATA3. PDEF was previously characterized as an Androgen Receptor cooperating factor in prostate, however its potential function as an ER cofactor is not explored yet. We investigated the mechanism of this ER/PDEF co expression and the role of PDEF in breast cancer cells. Using siRNA downregulations, mRNA quantification, and Chromatin IP, we discovered that the mechanism of ER/PDEF co expression implies a critical role for GATA3 as a negative regulator of PDEF expression. Moreover PDEF and GATA3 expressions are inversely correlated in ER positive Breast Cancer and cell lines. Kaplan-Meier survival analyses showed that these two factors could be useful prognosis markers for patients. We performed growth curves, cell cycle (FACs) and apoptosis (Annexin V) analyses of MCF7 cells transfected with a PDEF targeting siRNAs. The growth curves revealed an impaired proliferation rate of PDEF downregulated cells. Quantification of apoptosis in various conditions (basal, stress induced) demonstrated that PDEF is an essential survival factor for breast cancer cells. The loss of PDEF expression indeed triggers an increased proportion of apoptotic cells, especially in stress conditions such as hormone depletion or tamoxifen / fulvestrant treatments. The findings presented in this poster are evidences that designate PDEF as a potential new cofactor of ER and as an interesting therapeutic target, since its down regulation makes breast cancer cells more prone to undergo apoptosis in stress conditions. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2256. doi:1538-7445.AM2012-2256
Response to Letter Regarding Article, “Intracoronary Compared With Intravenous Bolus Abciximab Application in Patients With ST-Elevation Myocardial Infarction Undergoing Primary Percutaneous Coronary Intervention”
Authors: Holger Thiele, Josef Friedenberger, Ingo Eitel, Georg Fürnau, Eigk Grebe, Sandra Erbs, Axel Linke, Sven Möbius-Winkler, Gerhard Schuler, Kathrin Schindler, Dietmar Kivelitz
Journal: Circulation (2009)
Decline in Diarrhea Mortality and Admissions after Routine Childhood Rotavirus Immunization in Brazil: A Time-Series Analysis
Authors: Greice Madeleine Ikeda do Carmo, Catherine Yen, Jennifer Cortes, Alessandra Araújo Siqueira, Wanderson Kleber de Oliveira, Juan José Cortez-Escalante, Ben Lopman, Brendan Flannery, Lucia Helena de Oliveira, Eduardo Hage Carmo, Manish Patel
Journal: PLoS Medicine (2011)

In 2006, Brazil began routine immunization of infants <15 wk of age with a single-strain rotavirus vaccine. We evaluated whether the rotavirus vaccination program was associated with declines in childhood diarrhea deaths and hospital admissions by monitoring disease trends before and after vaccine introduction in all five regions of Brazil with varying disease burden and distinct socioeconomic and health indicators.National data were analyzed with an interrupted time-series analysis that used diarrhea-related mortality or hospitalization rates as the main outcomes. Monthly mortality and admission rates estimated for the years after rotavirus vaccination (2007-2009) were compared with expected rates calculated from pre-vaccine years (2002-2005), adjusting for secular and seasonal trends. During the three years following rotavirus vaccination in Brazil, rates for diarrhea-related mortality and admissions among children <5 y of age were 22% (95% confidence interval 6%-44%) and 17% (95% confidence interval 5%-27%) lower than expected, respectively. A cumulative total of ~1,500 fewer diarrhea deaths and 130,000 fewer admissions were observed among children <5 y during the three years after rotavirus vaccination. The largest reductions in deaths (22%-28%) and admissions (21%-25%) were among children younger than 2 y, who had the highest rates of vaccination. In contrast, lower reductions in deaths (4%) and admissions (7%) were noted among children two years of age and older, who were not age-eligible for vaccination during the study period.After the introduction of rotavirus vaccination for infants, significant declines for three full years were observed in under-5-y diarrhea-related mortality and hospital admissions for diarrhea in Brazil. The largest reductions in diarrhea-related mortality and hospital admissions for diarrhea were among children younger than 2 y, who were eligible for vaccination as infants, which suggests that the reduced diarrhea burden in this age group was associated with introduction of the rotavirus vaccine. These real-world data are consistent with evidence obtained from clinical trials and strengthen the evidence base for the introduction of rotavirus vaccination as an effective measure for controlling severe and fatal childhood diarrhea.
German private medical school to reform in face of criticism
Authors: Annette Tuffs
Journal: BMJ (2005)

Germany's first private university has announced that it will reform its medical school within the next three years, after severe criticism by the German Science Council (Wissenschaftsrat).

However, with the agreement of the Science Ministry of North Rhine-Westphalia, the Witten/Herdecke University (UWH), based near Dortmund and Bochum, will accept new medical students in summer 2006 despite the council's recent recommendation to stop accreditation of new students next year. Although there are now other private universities in Germany, UWH is the only one which has a medical faculty.

In July 2005, the science council had accredited all five faculties of UWH as academic institutions, but had pointed out that the medical faculty had severe deficits and either should undergo a fundamental reform in the next three years or close down (www.wissenschaftsrat.de).

“The main problem is the …
Ten-year survival in ursodeoxycholic acid-treated patients with primary biliary cirrhosis
Authors: Renée Eugénie Poupon, Anne-Marie Bonnand, Yves Chrétien, Raoul Poupon
Journal: Hepatology (1999)

Ursodeoxycholic acid (UDCA) treatment has been shown to increase survival without orthotopic liver transplantation (OLT) in patients with primary biliary cirrhosis (PBC) at 4 years. Whether this beneficial effect was maintained over the long term remained to be established. In a large cohort of UDCA-treated patients with PBC, we aimed to determine the 10-year outcome of these patients using two endpoints: (1) survival without OLT, and (2) survival. The cohort was comprised of 225 patients with PBC treated with UDCA (13-15 mg/kg/d) monitored from the beginning of treatment until time of last follow-up, OLT, or death. Because of the absence of a control group, survival without OLT was compared with survival predicted by the Mayo model (first 7 years), and observed 10-year survival with an estimation of survival of a standardized control cohort of the French population. Observed survival without OLT of UDCA-treated patients was significantly higher (P <.04) than survival predicted by the Mayo model. Observed survival was significantly lower (P <. 01) than survival predicted from the French population. Observed survival of noncirrhotic patients was not different (P >.9) from that of the French control population but survival of cirrhotic patients was significantly lower (P <.0001). Twenty-two patients died; 13 patients died of hepatic causes and 4 patients died after OLT. In conclusion, survival without OLT among patients treated with UDCA for PBC is higher than that of untreated patients, as predicted by the Mayo model. Ten-year survival among UDCA-treated patients is slightly lower than that of an age- and sex-matched general population, the difference mainly being explained by mortality among cirrhotic patients.
Taking a Sexual History
Authors: Domeena C. Renshaw
Journal: JAMA (1987)

This instructional 18-minute videotape by Marian Glasgow, a social worker at Boston University, is an interesting introduction to sexual history taking for all clinicians. Several simulated patients ask sexual questions; the narrator also neatly presents appropriate questions, should a sexual problem surface. A two-minute commentary by urologist Robert Krane, MD, stresses the changing ratio of etiologies of impotence, with emphasis today less on the psychogenic and more on the physical. A responsive, attentive audience interacts with Glasgow to highlight clinicians' acknowledged ambivalence about asking sexual questions, concerns about their own sexual ignorance, and difficulty in dealing with sexual behavior contrary to their own. These realities persist in 1987, and continuing education is needed from freshman to postgraduate level. However, the lampooning of physicians who make excuses for not taking a sexual history is offensive, overdone, and discouraging. One statement surprised me: that taking a sex history with the spouse present
Overview of ultraviolet radiation and cancer: what is the link? How are we doing?
Authors: M A Weinstock
Journal: Environmental Health Perspectives (1995)

Sun exposure has now been established as the most important avoidable cause of nonmelanoma skin cancer (NMSC) and melanoma. With specific reference to melanoma, there are several key issues that remain to be resolved. These include definition of the action spectrum, the importance of systemic effects of sun exposure, whether a tan is protective, the risk of tanning booth exposures, and the efficacy of sunscreens. Also the role, if any, of sun exposure in noncutaneous malignancies remains to be established. Melanoma incidence and mortality have increased dramatically over the past several decades, but these increases have now slowed, and for mortality among those 15 to 45 years of age, decreasing rates are now observed. Improving the coverage of the Surveillance, Epidemiology, and End Results (SEER) registries by requiring pathology laboratories in non-SEER areas to report cancers among SEER area residents will allow correct interpretation of these trends in the future at minimal cost. The available data on trends in NMSC incidence and mortality are suboptimal but suggest a pattern of declining mortality despite increasing incidence. Trends in NMSC morbidity have not been defined. Establishing NMSC registries in a few diverse sentinel areas would allow more reliable inference and monitoring. Techniques are being developed for reducing sun exposures and increasing early detection of skin cancers in the general population, but improved monitoring of incidence, mortality, and morbidity is required to monitor the effects of current and future ozone depletion and to evaluate prevention and early detection measures.
Assessment of Quality of Life in Outpatients With Advanced Cancer: The Accuracy of Clinician Estimations and the Relevance of Spiritual Well-Being—A Hoosier Oncology Group Study
Authors: Michael J. Fisch, Michael L. Titzer, Jean L. Kristeller, Jianzhao Shen, Patrick J. Loehrer, Sin-Ho Jung, Steven D. Passik, Lawrence H. Einhorn
Journal: Journal of Clinical Oncology (2003)

Purpose: To evaluate the association between quality-of-life (QOL) impairment as reported by patients and QOL impairment as judged by nurses or physicians, with and without consideration of spiritual well-being (SWB). Patients and Methods: A total of 163 patients with advanced cancer were enrolled onto a therapeutic trial, and cross-sectional data were derived from clinical and demographic questionnaires obtained at baseline, including assessment of patient QOL and SWB. Clinicians rated the QOL impairment of their patients as mild, moderate, or severe. Clinician-estimated QOL impairment and patient-derived QOL categories were compared. Correlation coefficients were estimated to associate QOL scores using different instruments. The analysis of variance method was used to compare Functional Assessment of Cancer Therapy–General scores on categorical variables. Results: There was no significant association between self-assessment scores and marital status, education level, performance status, or predicted life expectancy. However, a strong relationship between SWB and QOL was noted (P &lt; .0001). Clinician-estimated QOL impairment matched the level of patient-derived QOL correctly in approximately 60% of cases, with only slight variation depending on the method of categorizing patient-derived QOL scores. The accuracy of clinician estimates was not associated with the level of SWB. Interestingly, a subset analysis of the inaccurate estimates revealed an association between lower SWB and clinician underestimation of QOL impairment (P = .0025). Conclusion: Clinician estimates of QOL impairment were accurate in more than 60% of patients. SWB is strongly associated with QOL, but it is not associated with the overall accuracy of clinicians’ judgments about QOL impairment.
The Rorschach test in clinical psychology and law
Authors: H. N. Morse
Journal: JAMA (1967)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Intrasplenic pH in normal and phenylhydrazine‐induced anemic rats
Authors: L. T. Chen, P. K. Chiang
Journal: American Journal of Hematology (1981)

Abstract Direct measurement of the intrasplenic pH with a microcombination glass pH probe was performed in rats. The pH probe was inserted into the splenic pulp during measurements. In the normal rats, the pH was 7.21. However, in rats with hemolytic anemia induced by phenylhydrazine treatment, the pH decreased to 7.01. The level of lactic acid in the spleen was also determined. The lactic acid content in the spleen of anemic rats was found to be higher (1.20–1.44 μmoles/gm spleen) than that of normal rats (0.70–0.74 μmoles/gm spleen). The results suggest that the lower pH in the spleens of anemic rats may result from an increase in the lactic acid level in the spleen.
Climate Change Puts Children in Jeopardy
Authors: Rebecca Voelker
Journal: JAMA (2009)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Site-specific data confirm arsenic exposure predicted by the U.S. Environmental Protection Agency.
Authors: S Walker, S Griffin
Journal: Environmental Health Perspectives (1998)

The EPA uses an exposure assessment model to estimate daily intake to chemicals of potential concern. At the Anaconda Superfund site in Montana, the EPA exposure assessment model was used to predict total and speciated urinary arsenic concentrations. Predicted concentrations were then compared to concentrations measured in children living near the site. When site-specific information on concentrations of arsenic in soil, interior dust, and diet, site-specific ingestion rates, and arsenic absorption rates were used, measured and predicted urinary arsenic concentrations were in reasonable agreement. The central tendency exposure assessment model successfully described the measured urinary arsenic concentration for the majority of children at the site. The reasonable maximum exposure assessment model successfully identified the uppermost exposed population. While the agreement between measured and predicted urinary arsenic is good, it is not exact. The variables that were identified which influenced agreement included soil and dust sample collection methodology, daily urinary volume, soil ingestion rate, and the ability to define the exposure unit. The concentration of arsenic in food affected agreement between measured and predicted total urinary arsenic, but was not considered when comparing measured and predicted speciated urinary arsenic. Speciated urinary arsenic is the recommended biomarker for recent inorganic arsenic exposure. By using site-specific data in the exposure assessment model, predicted risks from exposure to arsenic were less than predicted risks would have been if the EPA's default values had been used in the exposure assessment model. This difference resulted in reduced magnitude and cost of remediation while still protecting human health.
Effect of Temperature on Hospital Admissions for Myocardial Infarction in a Subarctic Area
Authors: E. Sotaniemi, U. Vuopala, E. Huhti, J. Takkunen
Journal: BMJ (1970)

In a study of the effect of mean daily temperature on the admission and fatality rates of 771 patients with myocardial infarction the admission rate was found to be significantly higher on cold (temperature below 0 degrees C.) than on warm (temperature above 0 degrees C.) days. The fatality rate was almost equal in cold and warm weather. Thus the seasonal fluctuation in the admission rate of patients with myocardial infarction is probably due to a direct effect of environmental temperature.
Bone marrow involvement in mantle cell lymphoma
Authors: Kikkeri Naresh, Sarah Hartley, Donald Macdonald
Journal: American Journal of Hematology (2010)

A 68-year-old man presented with generalized lymphadenopathy.Lymph node biopsy showed mantle cell lymphoma.Staging bone marrow aspirate showed >50% small lymphoid cells (Top left Image and Top right Image-1003 objective).These cells had scanty weakly basophilic cytoplasm and minimally indented or cleaved nuclei.Bone marrow trephine biopsy section showed intertrabecular nodular infiltrates and low-volume interstitial infiltrate of small cleaved lymphoid cells with coarse chromatin (Middle left Image-103 objective; Middle right Image-603 objective).The cells expressed CD20 (Bottom left Image-203 objective), cyclin D1 (Bottom right Image-203 objective), CD5 and BCL2.They were negative for CD3, CD23, CD10, and BCL6.The features are typical of mantle cell lymphoma [1].
Ventricular Fusion Beats
Authors: William S. Sheils
Journal: JAMA (1967)

<h3>To the Editor:—</h3> I would like to take issue with the conclusion in the "Ventricular Fusion Beats"<b>201</b>:566, 1967). Dr. Yu-Chen Lee says, "The fusion beats should not be confused with... ventricular depolarization due to supraventricular rhythm." It is my contention that this is exactly what he has done. The tracing shows a high degree of atrioventricular (AV) block, but not complete. The beats having the configuration of right bundle branch block are probably conducted with a PR interval varying from (approx) 0.48 to 0.70 seconds. The absolute refractory period of the AV junctional tissue is somewhere between 1.08 and 1.28 seconds. Any P wave occurring with an R-P interval greater than 1.28 seconds is conducted. Any P wave occurring with an R-P interval less than 1.28 seconds is not conducted but is followed by a ventricular escape beat. Beats 4, 6, and 10 are typical fusion beats from
Upregulation of Angiotensin II Type 1 Receptor, Inflammatory Mediators, and Enzymes of Arachidonate Metabolism in Obese Zucker Rat Kidney
Authors: Zhong-Gao Xu, Linda Lanting, Nosratola D. Vaziri, Zhen Li, Lili Sepassi, Bernardo Rodriguez-Iturbe, Rama Natarajan
Journal: Circulation (2005)

Background— Severe obesity can result in proteinuria and progressive glomerulosclerosis in humans and experimental animals. The associated renal disease is ameliorated by weight reduction and/or blockade of the renin-angiotensin system. Various growth factors, cytokines, and lipid mediators are implicated in the pathogenesis of renal disease. To explore the possible involvement of these mediators in obesity-induced renal disease, we examined the expression of key enzymes of arachidonate metabolism and inflammatory genes in untreated and losartan-treated obese Zucker rats, a model of obesity, insulin resistance, and renal injury. Methods and Results— Seven-week-old male obese Zucker rats were randomized to losartan-treated (100 mg/L drinking H 2 O) and untreated groups, with lean Zucker rats as controls. After 4 months, RNA and protein were obtained from renal cortical tissue for relative reverse transcription–polymerase chain reaction, Western blots, and immunohistochemistry. Compared with the lean controls, obese Zucker rats showed significant glomerular matrix expansion and increased mRNA expression of the extracellular matrix protein fibronectin, inflammatory mediators interleukin-6 and monocyte chemoattractant protein-1, and 2 major enzymes of arachidonate metabolism, namely, 12/15-lipoxygenase and cyclooxygenase-2. This was associated with significant increases in p38 and extracellular signal-regulated kinase (ERK) 1/2 mitogen-activated protein kinase activities and marked upregulation of angiotensin II type 1 receptor (AT1R) mRNA and protein expression. These abnormalities and the associated glomerulopathy and proteinuria were prevented by administration of the AT1R blocker losartan. Conclusions— These findings indicate that obesity-induced glomerulopathy is associated with upregulation of key inflammatory mediators. These events are associated with and perhaps in part due to upregulation of AT1R, as evidenced by their reversal with AT1R blocker treatment.