tbuckley/cabot-search huggingface dataset:
518. Production of Recombinant Type 2 Adeno-Associated Virus in Bioreactors
Authors: Yves Durocher, Phuong Lan Pham, Gilles St-Laurent, Danielle Jacob, Brian Cass, Josephine Nalbantoglu, Amine Kamen
Journal: Molecular Therapy (2005)

Top of pageAbstract
Recombinant adeno-associated viruses (rAAV) represent a promising gene therapy vector that may be used in the treatment of diverse human diseases. The major obstacle to broaden their usage is the availability of a production process able to provide sufficient quantities for preclinical and human trials. We present here a successful process for rAAV-2 production in low-serum and serum-free medium performed in a 3L stirred-tank bioreactor. The process is based on the triple transfection of suspension-growing HEK293 cells employing polyethylenimine (PEI) as the DNA carrier. Production of AAV-GFP in a 3L serum-free medium bioreactor yielded titers of 5.1 |[times]| 1011 infectious viral particles (IVP), corresponding to 2 |[times]| 1012 viral genome (VG) or 6.8 |[times]| 1012 viral particles (VP). The cell-specific and total viral titers obtained in suspension culture were about three-fold higher to those obtained with adherent cells. The process is very simple and robust as it does not require a medium exchange, either before or after transfection, making it particularly attractive for the generation of large and homogeneous stocks of rAAV vectors.
Myocardial distribution of coronary blood flow in the isolated supported heart preparation
Authors: D. C. WARLTIER, H. F. HARDMAN, A. R. LADDU, P. SOMANI, G. J. GROSS
Journal: Cardiovascular Research (1975)

The regional distribution of myocardial blood flow was studied by use of radioactive microspheres (15 micro-meters [μm]) under control conditions and during an intracoronary infusion of norepinephrine (2 μg/min). In control experiments endocardial blood flow was generally greater than epicardial flow; during administration of norepinephrine the endocardium received significantly less blood flow. It is proposed that normally the endocardium is well perfused, but under conditions of stress, eg, norepinephrine infusion in the presence of a constant coronary blood flow, decreased endocardial blood flow results.
852-1 Clinical outcomes in coronary angioplasty centers with off-site versus on-site cardiac surgery capabilities: A preliminary report from the American College of Cardiology — National Cardiovascular Data Registry
Authors: Michael A Kutcher, Lloyd W Klein, Thomas P Wharton, Robert J Applegate, Ralph G Brindis, Richard E Shaw, William S Weintraub
Journal: Journal of the American College of Cardiology (2004)
P218tPA in combination with MMP-10 a new effective strategy for thrombolysis in ischemic stroke with neuroprotective effect
Authors: J Orbe, C Roncal, JA Rodriguez, A Salicio, J Montaner, A Rosell, S Martinez De Lizarrondo, D Vivien, JA Paramo
Journal: Cardiovascular Research (2014)

Purpose: Early reperfusion using tissue-type plasminogen activator (tPA) is the only therapeutic strategy to treat focal cerebral ischemia with proven efficacy in stroke patients. Moreover, efficacy of tPA is not sufficient since only a third of tPA-treated patients recover and are free from disability. tPA has also shown neurotoxicity in experimental models of cerebral ischemia. Therefore, a combination treatment that would reduce the deleterious effects of tPA, while maintaining or improving its efficacy, might extend its usability and efficacy.
(23) Nickel dermatitis—a follow-up study
Authors: H. Schubert, Erika Prater
Journal: British Journal of Dermatology (1987)

Nickel dermatitis has become the most frequent allergic contact dermatitis in the region of Erfurt (Germany). Between 1982 and 1986 the incidence in our patch test clinic increased from 13·7% (18·3% female, 5·7% male) to 21·5% (27·4% female, 80% male). Eighty per cent of the female patients were under 30 years of age and 42% under 20 years of age. Primary nickel sensitization was due to fancy jewellery and wrist watches in 62% and dressing buckles in 14%. Nickel sensitivity was occupationally acquired in 25%; half of the workers were galvanizers. Three years after initial patch testing all cases of 2 + and 3 + reactions to nickel sulphate in 5% petrolatum remained positive. Twenty out of 32 patients were free of skin lesions. Eight still had mild eczema of the hands. Twenty-three have changed their job because of the nickel allergy and are now working as secretary, referee, textile shop assistant, telephone operator, woodworker and textile manufacturer. A positive patch test to nickel can become negative but this is rare. Normally rehabilitation is possible by avoidance of numerous and intensive contacts and is easier than in chromate dermatitis. Polysensitization renders or prevents healing and occupational rehabilitation.
Pulmonary Disease Complicating Intermittent Therapy With Methotrexate
Authors: Albert M. Clarysse
Journal: JAMA (1969)

A severe but self-limited respiratory illness characterized by fever, cough, dyspnea, cyanosis, and bilateral pulmonary infiltrates developed in seven consecutive patients with acute lymphoblastic leukemia who received intermittent therapy with methotrexate, while in clinical and hematologic remission of the leukemia. No infectious agent could be identified, and a moderate blood eosinophil count accompanied the illness in five of the seven patients. Lung biopsy performed on one patient revealed an allergic type granulomatous reaction. It is suggested but not proved that the pulmonary disease represented an adverse reaction to methotrexate therapy.
Recommendations from the findings by the RCGP oral contraception study on the mortality risks of oral contraceptive users.
Authors: E V Kuenssberg, C R Kay, J Dewhurst, R J Booth
Journal: BMJ (1977)

A study conducted by the Royal College of General Practitioners turned up evidence that oral contraceptive users compared with nonusers have an increased risk of cardiovascular death of 1/5000 annually.  This heightened risk applies particularly to women over 35 women who smoke and women who have used orals for 5 years or more continuously.  The risk for women under 35 is not great enough that they should change their contraceptive practices.  It is assumed that age 35 is an arbitrary turning point;  the risk is assumed to increase gradually at earlier ages.  Present data do not indicate whether lower dosages of estrogen offer advantages over pills containing 50 mcg.
Nurse is arrested over deaths at Stepping Hill hospital
Authors: J. Wise
Journal: BMJ (2011)

A registered nurse has been arrested on suspicion of murder after the deaths of three patients at Stepping Hill hospital in Stockport, Greater Manchester.

At the time of going to press Rebecca Jane Leighton remains in police custody for questioning, and the investigation is ongoing. 

Greater Manchester police were first called to the hospital on 12 July after an experienced nurse noticed abnormally high numbers of patients on her ward with low blood sugar levels and discovered that ampoules of saline solution had been tampered with. It is understood that insulin had been used to contaminate a …
Time to evaluate focal therapy
Authors: P. Cathcart, H. U. Ahmed, C. Moore, M. Emberton
Journal: BMJ (2010)

Smith and colleagues’ findings on quality of life after diagnosis and treatment of localised prostate cancer1 are neither new nor unexpected.2 3

While much resource has gone into refining both radiotherapeutic and surgical approaches to care, the key question is: has increased specification decreased the toxicity of prostate cancer …
Swearing to care: the resurgence in medical oaths
Authors: B. Hurwitz, R. Richardson
Journal: BMJ (1997)

We are witnessing a resurgence of professional interest in medical oaths and codes of conduct. In the United Kingdom the General Medical Council has reissued its professional code and, together with the BMA, the royal colleges, and other organisations, has published a document on the “core values” of medical practice.1 2 There has been discussion of the role of oath taking at the end of medical training, and the BMA has drafted a new Hippocratic Oath on behalf of the World Medical Association (see third box).3 4 5 6 7 8 9 10 11 The American Medical Association has this year commemorated the 150th anniversary of its 1847 Code of Ethics with an extensive debate on the relevance of oaths and codes to modern practice.12 13 14

#### Declaration of Geneva

“At the time of being admitted as a Member of my Profession:

I solemnly pledge myself to consecrate my life to the service of humanity;

I will give to my teachers the respect and gratitude which is their due;

I will practice my profession with conscience and dignity;

The health of those in my care will be my first consideration;

I will respect the secrets that are confided in me, even after the patient has died;

I will maintain by all the means in my power, the honour and the noble traditions of my profession;

My colleagues will be my sisters and brothers;

I will not permit considerations of age, disease or disability, creed, ethnic origin, gender, nationality, political affiliation, race, sexual orientation, or social standing to intervene between my duty and my patient;

I will maintain the utmost respect for human life from its beginning, even under threat, and I will not use my specialist knowledge contrary to the laws of humanity;

I make these promises solemnly, freely, and …
Two Scottish surgeons suspended
Authors: C. Dyer
Journal: BMJ (1998)

Two of the three consultant general surgeons at a Scottish hospital have been suspended from duty pending investigations into their clinical practices after concerns were raised by colleagues.

The unrelated suspensions, which happened within three weeks of each other, have plunged Stracathro Hospital, near Brechin in Angus, into chaos, causing management to suspend emergency operations for at least three months. A surgeon from Ninewells Hospital in Dundee has been seconded to Stracathro to plug the gap.

Mr Douglas Irving, who …
The peripheral metabolism of triiodothyronine in normal subjects and in patients with hyperthyroidism
Authors: Kenneth A. Woeber, Richard J. Sobel, Sidney H. Ingbar, Kenneth Sterling
Journal: Journal of Clinical Investigation (1970)

In order to assess the contribution of 3.3',5-triiodo-L-thyronine (T(3)) to overall thyroid hormone economy, conjoint measurements of the kinetics of peripheral T(3) metabolism and the total concentration of T(3) in serum were made in a group of normal subjects and in a group of patients with hyperthyroid Graves' disease. As judged from the disappearance of trichloroacetic acid-precipitable (131)I from serum after a single intravenous dose of labeled T(3), the following mean values were obtained in the normal subjects: volume of distribution, 43 liters or 0.62 liter/kg; fractional turnover rate. 52% per 24 hr: clearance rate, 22.3 liters/24 hr: and absolute disposal rate, 60 mug/24 hr. In the patients with untreated hyperthyroidism, values for all these functions were greatly increased. After treatment, the volume of T(3) distribution returned to normal but the fractional turnover rate remained abnormally rapid.
A NEW URETHROSCOPE TO BE USED WITH THE AID OF WATER DISTENSION.
Authors: GEORGE WALKER
Journal: JAMA (1907)

The accompanying cut represents a urethroscope based on the broad principles of the cystoscope, but so modified as to enable one to get a lateral view of the urethra and not that of the collapsed wall which we ordinarily see. At first I employed the ordinary tubes in which a window had been cut immediately opposite the prism, but this proving unsatisfactory, it occurred to me to distend the urethra with water and to discard the tube entirely. I then devised the metal cap to be fitted into the meatus. Water distension has proved to be very advantageous and superior in many ways to the tube. <h3>DESCRIPTION OF INSTRUMENT.1</h3> The urethroscope is No. 15 French; its full length is 20 cm. (8 in.); its length from the outer lens to the prism is 17.5 cm. (7 in.); it focuses at ⅛ inch, at which distance the amplification is 3 diameters.
Examining the anterior right kidney. Frequent lack of appreciation in examination of the right upper quadrant
Authors: A. Walzer
Journal: JAMA (1979)

An anteriorly positioned lower pole of the right kidney is often unappreciated on physical examination. Yet, on review of a large number of abdominal sonograms, more than 12% of the patients had a right kidney with a lower pole sufficiently anterior in position so as to lie at the same depth as the anterior surface of the liver. In a group of patients referred for ultrasound evaluation of hepatomegaly, 20% proved to have a normal liver and an anteriorly positioned lower pole of the right kidney. (<i>JAMA</i>242:2320-2321, 1979)
Thalidomide may be a mutagen
Authors: W G McBride, A P Read
Journal: BMJ (1994)

address the issue of selection bias in their study population.Hypertension in black people may be diagnosed at a more advanced stage than in the general population, and referrals to a specialist clinic may also differ by ethnic group.This would mean that the true degree of hypertension was greater in the black people studied.These selection biases can be overcome by a population based study.In such a study we showed that average resting systolic blood pressure is 6 mm Hg higher in men of black African descent and 17 mm Hg higher in women of black African descent than in the general population.2Further, we showed that the nocturnal fall in blood pressure is less in black than white people, even when resting blood pressure is taken into account.The overall burden of hypertension for a similar blood pres- sure would therefore be greater in black than in white people.People of black African descent have a greater mortality from stroke,3 more left ventricular hypertrophy,4 and more renal damage5 than white people.Racial differences in cardiac structure alone cannot account for this.A more plausible explanation is that ethnic differences in hypertensive load are inadequately characterised by current methods of blood pressure measurement.
Is Acute Hemodynamic Response a Predictor of Long-Term Outcome in Cardiac Resynchronization Therapy?
Authors: Frits W. Prinzen, Patrick Houthuizen, Margot D. Bogaard, Berry van Gelder, Frank Bracke, Maarten J.M. Cramer, Geert E. Leenders, Mathias Meine
Journal: Journal of the American College of Cardiology (2012)
Hot Topics in Infection and Immunity in Children VIII
Authors: Myles B. Abbott
Journal: JAMA (2012)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Chemical Toxicity Screening
Authors: Bridget M. Kuehn
Journal: JAMA (2012)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Sodium Zirconium Cyclosilicate in Hyperkalemia
Authors: David K. Packham, Henrik S. Rasmussen, Philip T. Lavin, Mohamed A. El-Shahawy, Simon D. Roger, Geoffrey Block, Wajeh Qunibi, Pablo Pergola, Bhupinder Singh
Journal: New England Journal of Medicine (2014)

Hyperkalemia (serum potassium level, >5.0 mmol per liter) is associated with increased mortality among patients with heart failure, chronic kidney disease, or diabetes. We investigated whether sodium zirconium cyclosilicate (ZS-9), a novel selective cation exchanger, could lower serum potassium levels in patients with hyperkalemia.
Erythrodermic Epidermolysis Bullosa Acquisita
Authors: Stefanie Häfliger, Hans-Wilhelm Klötgen, Michael Horn, Helmut Beltraminelli, Luca Borradori
Journal: JAMA Dermatology (2015)

Our case was striking because the patient initially showed features suggestive of either a severe drug reaction or a paraviral eruption, but immunopathological studies were diagnostic for EBA.Our observation provides a further example about the polymorphous and misleading presentations of EBA.Hence, EBA should be considered as a rare cause of erythroderma.
Pharmacotherapy and Community Psychiatric Practice
Authors: H. G. WHITTINGTON, RORRY ZAHOUREK, LESLIE GREY
Journal: American Journal of Psychiatry (1969)

Thirty-two outpatients at a community mental health center were treated in a double-blind study using thioridazine and a placebo. The target symptom of anxiety was observed and measured prior to treatment and at weekly interviews for four weeks. It was found that treatment with thioridazine, compared to placebo, produced reduction of anxiety and increased motivation to remain in treatment and follow through with treatment recommendations. The authors stress the implications of their findings for community psychiatric practice.
Combined Instrument and Sponge Stand and Sterilizing Apparatus.
Authors: F. F. LAWRENCE
Journal: JAMA (1900)

The accompanying photographs show two satisfactory additions to the operating-room. The first is a combined instrument and sponge-tray stand. It consists of an enameled-iron leaf or table on one side for the instruments, and on the opposite of two rings which support two porcelain basins of large size. One of these is used for small sponges, the other for large ones. At the bottom of the stand is another ring supporting a deep basin, which is also of enameled iron. To the surgeon, who operates with little or no assistance, this table will be of especial advantage; the basin at the bottom is a receptacle for soiled sponges. In my work I handle all instruments, sponges, in fact everything, using no assistant. I place my instruments either in trays on the solid leaf or on a sterilized towel spread over the leaf—the table standing at my right —with the small
Detection of Peripheral Arterial Disease in Primary Care
Authors: Kenneth Ouriel
Journal: JAMA (2001)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Sequence of Fibrinogen Proteolysis and Platelet Release after Intrauterine Infusion of Hypertonic Saline
Authors: H. L. Nossel, J. Wasser, K. L. Kaplan, K. S. Lagamma, I. Yudelman, R. E. Canfield
Journal: Journal of Clinical Investigation (1979)

Plasma fibrinopeptide B (Bβ1-14 or FPB) immunoreactivity was studied by radioimmunoassay in patients who received intrauterine infusion of hypertonic saline to terminate pregnancy. FPB immunoreactivity increased with thrombin treatment (TIFPB) suggesting the presence of a larger FPB-containing peptide, since purified FPB is not altered by thrombin, whereas thrombin increases the immunoreactivity of Bβ1-42 (which includes FPB) 10-fold. TIFPB immunoreactivity in plasma, drawn 4 h after hypertonic saline infusion eluted from Sephadex G-50 similarly to isolated Bβ1-42. Streptokinase, incubated with normal plasma progressively generated TIFPB immunoreactivity, which showed a major component which eluted from Sephadex G-50 similarly to Bβ1-42. Streptokinase generated TIFPB much more rapidly in reptilase-treated plasma that contains fibrin I, (which still includes FPB), indicating that fibrin I is preferred over fibrinogen as a substrate for plasmin cleavage of arginine (Bβ42)-alanine (Bβ43). Serial studies were then made in 10 patients receiving intrauterine hypertonic saline. Fibrinopeptide A (FPA) levels rose immediately, reached a peak between 1 and 2 h, were declining at 4 h, and were normal at 24 and 48 h. TIFPB levels rose slightly in the 1st h, reached a peak at 4 h, and had returned to base-line values at 24 h. Serum fibrinogen degradation product levels were unchanged at 1 h, reached their highest level at 4 h, and were still markedly elevated at 24 and 48 h. Fibrinogen levels dropped slightly being lowest at 4 and 24 h. Platelet counts declined in parallel with the fibrinogen levels over the first 4 h, but continued to decrease through 48 h. Beta thromboglobulin (βTG) levels generally paralleled FPA levels whereas platelet factor 4 (PF4) levels showed only slight changes. The data indicate that immediately after intrauterine hypertonic saline infusion thrombin is formed that cleaves FPA from fibrinogen to produce fibrin I and releases βTG and PF4 from platelets. Later plasmin cleaves Bβ1-42 from fibrin I to produce fragment X, which is further degraded to form serum fibrinogen degradation products. This sequence of proteolysis indicates that plasmin action on fibrin I serves as a mechanism that regulates fibrin II formation by removing the Bβ chain cleavage site, which is required for thrombin action in converting fibrin I to fibrin II.