From Finger Pricks to Point-and-Click
Authors: Manikandan Narayanan, John S. Tsang
Journal: Immunity (2013)

In this issue of Immunity, Obermoser et al., 2013Obermoser G. Presnell S. Domico K. Xu H. Wang Y. Anguiano E. Thompson-Snipes L. Ranganathan R. Zeitner B. Bjork A. et al.Immunity. 2013; 38 (this issue): 831-844Abstract Full Text Full Text PDF PubMed Scopus (210) Google Scholar systematically analyze and compare the blood-transcriptomic response of the pneumococcal and influenza vaccines in humans over multiple time points spanning hours to tens of days. They then present web-based interactive figures that facilitate exploration of this large, complex data set. In this issue of Immunity, Obermoser et al., 2013Obermoser G. Presnell S. Domico K. Xu H. Wang Y. Anguiano E. Thompson-Snipes L. Ranganathan R. Zeitner B. Bjork A. et al.Immunity. 2013; 38 (this issue): 831-844Abstract Full Text Full Text PDF PubMed Scopus (210) Google Scholar systematically analyze and compare the blood-transcriptomic response of the pneumococcal and influenza vaccines in humans over multiple time points spanning hours to tens of days. They then present web-based interactive figures that facilitate exploration of this large, complex data set. Advances in high-throughput multiplexed technologies for measuring states of biological systems from molecules, to cells, to whole organisms are rapidly turning biology into a “big data” science. Immunology is no exception. Although the immune system and its response to perturbations such as infection and cancer are invariably complex, involving intricate molecular and cellular orchestrations over space and time, the increasingly comprehensive view offered by global studies of immunity complements more traditional approaches to chip away at this complexity. Microarray profiling of peripheral blood before and after yellow fever and influenza vaccination, for instance, has revealed both expected (e.g., plasmablast) and new response signatures and has led to novel hypotheses on the genetic regulation of antibody and CD8+ T cell responses, which in turn could help facilitate rational design of new vaccines (Pulendran et al., 2010Pulendran B. Li S. Nakaya H.I. Immunity. 2010; 33: 516-529Abstract Full Text Full Text PDF PubMed Scopus (289) Google Scholar). The extent to which immune responses vary among different vaccines, however, remains largely unexplored at the genome-wide scale. In this issue of Immunity, Obermoser et al., 2013Obermoser G. Presnell S. Domico K. Xu H. Wang Y. Anguiano E. Thompson-Snipes L. Ranganathan R. Zeitner B. Bjork A. et al.Immunity. 2013; 38 (this issue): 831-844Abstract Full Text Full Text PDF PubMed Scopus (210) Google Scholar systematically and comprehensively compare the blood-transcriptomic responses of two widely used vaccines: the pneumococcal vaccine, composed of polysaccharide extracts, and the influenza split vaccine, which comprises inactivated viral components. In addition to providing a comprehensive analysis of the shared and distinct transcriptome signatures induced by two important vaccines, this work contributes a rich data set for the investigation of vaccine responses in humans. However, exploration of such large data sets requires custom bioinformatics analysis, which is typically accessible only to individuals with computational and statistical expertise. Here, the authors provide an innovative solution: for the first time in Immunity (and to the best of our knowledge, in any scientific journal), readers can further explore the data (not just the images) presented in all of the figures through an aesthetically appealing and functionally rich set of web tools called interactive figures (iFigures). These interactive data-browsing and analysis tools render such complex data sets immediately accessible to a diverse biological audience. The design of this study is noteworthy (Figure 1). In addition to the typical time points (days 0, 1, 3, and 7) profiled in previous studies of yellow fever and influenza vaccination, this work investigates longer-term responses by sampling from days 10, 14, 21, and 28. To examine innate responses over a dense set of early time points in the scale of hours after vaccination, the authors profile minute amounts of blood from finger-prick samples in a separate cohort of individuals. Also included is a parallel placebo arm with the same set of time points wherein saline was administered in lieu of the vaccine. The lack of substantial response activity following saline injection provides reassurance that the data-generation platforms are robust; it also indicates that intramuscular injection elicits minimal transcriptomic activities in the blood; thus, those observed in the main arms are vaccine specific. Another nice feature is the inclusion of an extra prevaccination time point (day −7) for obtaining more robust baseline estimates. The cohort sizes are relatively small, making robust inference of correlates of immunogenicity and control for confounding by outlier individuals difficult. However, the sample size is sufficient for deriving robust vaccine signatures, and the careful design and methodology of the study will serve as an invaluable reference for future blood-transcriptomic studies in humans, such as ones involving pediatric subjects, dense time points, and minute blood volumes. A major challenge in any study involving large-scale data is the transformation of such data sets into biological insights, and one of the main obstacles is the curse of the high dimensionality of such data sets. The authors address this in a couple of ways. First, they reduce the dimensionality of transcriptomic responses from tens of thousands of genes to 62 functionally annotated gene modules, which are sets of coexpressed genes derived from multiple independent blood-transcriptomic data sets (Chaussabel et al., 2008Chaussabel D. Quinn C. Shen J. Patel P. Glaser C. Baldwin N. Stichweh D. Blankenship D. Li L. Munagala I. et al.Immunity. 2008; 29: 150-164Abstract Full Text Full Text PDF PubMed Scopus (490) Google Scholar). Similarly, they draw insights from a host of early responding genes by comparing them to a manageable set of innate transcriptomic signatures, which are obtained from the stimulation of in vitro blood cell culture with diverse stimuli, including cytokines, Toll-like receptor (TLR) ligands, live viruses, and killed bacteria. Second, iFigures provide a point-and-click web interface to expand the static views of traditional figures and allow the reader to explore the data from different perspectives, thus offering a great level of flexibility and transparency. The reader can now test the robustness of the authors’ conclusions to different p value or fold-change cutoffs, inspect the flow-cytometry gating details when examining figures depicting cell-population frequencies, and select and further investigate the genes inside modules of interest (Figure 1)—these are but a few of the myriad exploratory possibilities. Although releasing the raw data, as the authors are also doing with this work, is crucial for enabling unrestricted exploratory analysis, model building, integration with data from other studies, and further hypothesis generation, iFigures extend the reach of many of these exercises from computational experts to biologists with limited bioinformatics expertise. The comparative exercise undertaken in the paper revealed that within hours both vaccines can induce meaningful transcriptomic responses in the blood, with interferon and inflammatory/TLR-inducible genes predominant for the influenza and pneumococcal vaccines, respectively. For both vaccines plasmablast responses were apparent by day 7, but the pneumococcal vaccine had a more sustained response that lasted until day 10, as seen in both microarray and flow-cytometry data. These interesting observations raise further questions: e.g., how do differences in early responses affect the duration and level of the plasmablast response? Answers to this question can point to strategies for designing more potent vaccines. The current data also leave open the question of the mechanisms that drive the marked differences between the vaccines. Expression profiling of sorted cell subsets from the influenza vaccinees indicated that the interferon signature originated from neutrophils and monocytes, but which cell subset produced the interferon is less clear. A related question is where neutrophil stimulation took place—at the site of injection or further downstream? To begin to answer these questions, future studies could consider simultaneous profiling of blood and tissue biopsies from the injection site; utilizing mouse models from which tissue samples can be obtained more readily could also prove valuable. In general, correlating responses in tissues to those in blood would be highly informative and could start to reveal molecular and cellular mechanisms that give rise to blood response signatures. Data from this and other large-scale studies of human immunity together with ongoing efforts (e.g., those from the Human Immunology Project Consortium and the National Institutes of Health’s Center for Human Immunology, Autoimmunity, and Inflammation [Leslie, 2010Leslie M. Science. 2010; 327: 1573Crossref PubMed Scopus (18) Google Scholar]) will begin to provide increasingly comprehensive characterizations of the human immune system, e.g., before and after vaccine and drug intervention in healthy and disease-affected individuals. Proper integration of these dense data sets will start to provide a human “immune state” atlas—akin to the Connectivity Map for functional genomics and drug discovery (Lamb et al., 2006Lamb J. Crawford E.D. Peck D. Modell J.W. Blat I.C. Wrobel M.J. Lerner J. Brunet J.P. Subramanian A. Ross K.N. et al.Science. 2006; 313: 1929-1935Crossref PubMed Scopus (3510) Google Scholar)—useful for probing and comparison of immune statuses under diverse settings and for reverse engineering functional interactions among immune-system components based on correlations among the observed phenotypes across individuals (Marbach et al., 2012Marbach D. Costello J.C. Küffner R. Vega N.M. Prill R.J. Camacho D.M. Allison K.R. Kellis M. Collins J.J. Stolovitzky G. DREAM5 ConsortiumNat. Methods. 2012; 9: 796-804Crossref PubMed Scopus (1099) Google Scholar). In addition to proper design (e.g., sufficiently large cohort sizes) and execution at the individual study level, achieving this goal requires overcoming several obstacles, such as the standardization of experimental practices and technology platforms, the identification of confounding covariates, and the development of approaches for normalization across studies, as well as agreeing to common data-dissemination and replication standards. Some of these, such as data-processing standards and sharing policies, can be partially borrowed from similar efforts in related fields (e.g., The Cancer Genome Atlas [Chin et al., 2011Chin L. Andersen J.N. Futreal P.A. Nat. Med. 2011; 17: 297-303Crossref PubMed Scopus (454) Google Scholar]); others need further research innovation as well as collaboration and dialog across the research community. The biggest challenge and opportunity, however, lies in the development of novel concepts and approaches to turn such data compendia into immunological insights and predictive constructs for unseen perturbations. For example, predictive models have been built for disease prognosis (van ’t Veer et al., 2002van ’t Veer L.J. Dai H. van de Vijver M.J. He Y.D. Hart A.A. Mao M. Peterse H.L. van der Kooy K. Marton M.J. Witteveen A.T. et al.Nature. 2002; 415: 530-536Crossref PubMed Scopus (7765) Google Scholar) and are being explored at levels ranging from molecular interactions to whole cells (Bonneau et al., 2007Bonneau R. Facciotti M.T. Reiss D.J. Schmid A.K. Pan M. Kaur A. Thorsson V. Shannon P. Johnson M.H. Bare J.C. et al.Cell. 2007; 131: 1354-1365Abstract Full Text Full Text PDF PubMed Scopus (244) Google Scholar; Karr et al., 2012Karr J.R. Sanghvi J.C. Macklin D.N. Gutschow M.V. Jacobs J.M. Bolival Jr., B. Assad-Garcia N. Glass J.I. Covert M.W. Cell. 2012; 150: 389-401Abstract Full Text Full Text PDF PubMed Scopus (854) Google Scholar). Extending these by incorporating key elements of immunity (e.g., cell-cell interactions; spatial and temporal considerations) and proper abstraction and modeling across different biological scales will help usher in an era of immunology in which the systematic collection of large-scale data sets such as the one presented in this study is combined with computational and mathematical modeling to systematically chip away at the complexity of the human immune system in health and disease.