tbuckley/cabot-search huggingface dataset:
P08.02 * IDENTIFICATION AND QUANTIFICATION OF CSF MALIGNANT CELLS BY THE CELLSEARCH(R) TECHNOLOGY IN PATIENTS WITH LUNG LEPTOMENINGEAL METASTASIS
Authors: M. Blonski, B. Wittwer, G. Faure, L. Simon, M. D. C. Bittencourt, Q. Tu, D. Larrieu, E. Le Rhun, L. Taillandier
Journal: Neuro-Oncology (2014)

INTRODUCTION: Diagnosis of lung leptomeningeal metastasis (LM) remains difficult. The usual diagnostic methods of cytomorphological assessment of cerebro-spinal fluid (CSF) and gadolinium-enhanced MRI lack both specificity and sensitivity. The Veridex CellSearch® technology is validated in detection of circulating tumor cells (CTC) in blood and in the follow-up of breast, prostate and colorectal cancer. Our aim was to adapt this technology for detection and quatification of tumor cells in the CSF of lung cancer patients with LM. METHODS: Patients with suspected lung LM from a french neuro-oncological center (Nancy) were prospectively included. The CSF samples were collected on traditional cytology tubes (1mL) and on CellSave® Preservative tubes (4 mL). RESULTS: 12 patients with clinical symptoms and/or radiological criteria suggestive of lung LM (5 at diagnosis/7 at recurrence) underwent CSF analysis with conventional cytology and with Veridex Cellsearch® technology. Conventional CSF cytomorphological analysis was positive in 5 patients (with a median of 13 tumor cells/mm3; range 10 to 25 tumor cells/mm3) whereas the assessment with Veridex Cellsearch® technology was positive in 12 patients. Quantitative analysis with the Veridex CellSearch® technology showed a median of 203 tumor cells/5mL of CSF (range 1 to 1500 tumor cells per 5 mL of CSF). CONCLUSIONS: In contrast to the current gold standard cytomorphological analysis, this new approach seems more sensitive and allowed a quantification of CSF tumor cells in lung LM. This methodology could be useful for earlier diagnosis of lung LM and for follow-up. A large prospective study is required.
MITRAL E/E&amp;apos; RATIO AS AN INDEPENDENT PREDICTOR FOR CARDIOVASCULAR EVENTS IN PATIENTS WITH ATRIAL FIBRILLATION
Authors: Satoshi Tsujimoto, Yoko Miyasaka, Yoshinobu Suwa, Fumio Yuasa, Hirofumi Maeba, Toshiji Iwasaka
Journal: Journal of the American College of Cardiology (2012)
Leiomyoma of the Bronchus
Authors: S. I. Turkington, G. A. Scott, T. B. Smiley
Journal: Thorax (1950)

The bronchial tree is a most unusual site for a leiomyoma, and the rarity of such a tumour justifies the presentation of this case. CASE REPORTThe patient was a housewife aged 57 years.In 1942 she suffered from severe metrorrhagia stated to be caused by uterine fibroids.A subtotal hysterectomy was performed in a country hospital, but the pathological details of this are not available.Since this operation and until the onset of the illness to be described, the patient was in excellent health.In July 1949, whilst a front-seat passenger in one of two colliding motor-cars, the patient's left side came into violent contact with the dashboard.Sustaining apparently minor abrasions of the left arm and recovering from the shock, she went home feeling well.That evening she noticed gradually increasing breathless- ness and, in the early hours of , , _ F .w , ._
In vitro and in vivo activation of endothelial cells by colony-stimulating factors.
Authors: F Bussolino, M Ziche, J M Wang, D Alessi, L Morbidelli, O Cremona, A Bosia, P C Marchisio, A Mantovani
Journal: Journal of Clinical Investigation (1991)

This study was designed to identify the set of functions activated in cultured endothelial cells by the hematopoietic growth factors, granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage-colony-stimulating factor (GM-CSF), and to compare them with those elicited by prototypic cytokines active on these cells. Moreover, indications as to the in vivo relevance of in vitro effects were obtained. G-CSF and GM-CSF induced endothelial cells to proliferate and migrate. In contrast, unlike appropriate reference cytokines (IL-1 and tumor necrosis factor, IFN-gamma), G-CSF and GM-CSF did not modulate endothelial cell functions related to hemostasis-thrombosis (production of procoagulant activity and of platelet activating factor), inflammation (expression of leukocyte adhesion molecule-1 and production of platelet activating factor), and accessory function (expression of class II antigens of MHC). Other colony-stimulating factors (IL-3 and macrophage-colony-stimulating factor) were inactive on all functions tested. In comparison to basic fibroblast growth factor (bFGF), G-CSF and GM-CSF induced lower maximal proliferation of endothelial cells, whereas migration was of the same order of magnitude. G-CSF and GM-CSF stimulated repair of mechanically wounded endothelial monolayers. Exposure to both cytokines induced shape changes and cytoskeletal reorganization consistent with a migratory phenotype. To explore the in vivo relevance of the in vitro effects of these cytokines on endothelium, we studied the angiogenic activity of human G-CSF in the rabbit cornea. G-CSF, but not the heat-inactivated molecule, had definite angiogenic activity, without any sign of inflammatory reactions. G-CSF was less active than bFGF. However, the combination of a nonangiogenic dose of bFGF with G-CSF resulted in an angiogenic response higher than that elicited by either individual cytokines. Thus, G-CSF and GM-CSF induce endothelial cells to express an activation/differentiation program (including proliferation and migration) related to angiogenesis.
Resistance to fever induction and impaired acute-phase response in interleukin-1β-deficient mice
Authors: Hui Zheng, Daniel Fletcher, Wieslaw Kozak, Minghao Jiang, Kathryn J. Hofmann, Carole A. Corn, Darlusz Soszynski, Christina Grabiec, Myrna E. Trumbauer, Alan Shaw, Matthew J. Kostura, Karla Stevens, Hugh Rosen, Robert J. North, Howard Y. Chen, Michael J. Tocci, Matthew J. Kluger, Lex H.T. Van der Ploeg
Journal: Immunity (1995)
Patients' satisfaction with the NHS is growing, survey shows
Authors: Andrew Cole
Journal: BMJ (2006)

At a time of growing concern about the future of the NHS, patients' satisfaction with the care they get in hospital has risen, shows the latest survey by the NHS watchdog body, the Healthcare Commission.

The survey, of more than 80 000 inpatients in 169 trusts in England, found that 92% rated their care as excellent, very good, or good. The previous year this percentage was 90%.

Nearly four in five patients (79%) said they were always treated with dignity and respect. Furthermore, the number waiting more than four hours to be admitted to a ward or bed from emergency departments has dropped.

The survey, which …
Trend tests for proportional responses in developmental toxicity experiments.
Authors: V M Chinchilli, B C Clark
Journal: Environmental Health Perspectives (1989)

The data from developmental toxicity experiments usually are very difficult to analyze statistically because of the lack of independence among littermates and the random nature of the litter size. Only a few of the models that have been proposed in the literature have accounted for both of these features. One of the models proposed by Van Ryzin is invoked to construct a test of trend (dose response). The construction is achieved via a statistical technique called isotonic regression, which is applied to the moment estimators derived by Van Ryzin. The trend test based on isotonic regression is relatively straightforward to calculate, and when the number of dose groups (including control) is four or less, the significance of the observed result is easily determined. An example, in which fetolethality is the end point of interest, demonstrates the test.
Male cell microchimerism in normal and diseased female livers from fetal life to adulthood
Authors: Catherine Guettier, Mylène Sebagh, Jérôme Buard, Danielle Feneux, Monique Ortin-Serrano, Michele Gigou, Viviane Tricottet, Michel Reynès, Didier Samuel, Cyrille Féray
Journal: Hepatology (2005)

Abstract Male microchimerism is frequent in the adult female liver and is attributed to fetal cells originating from previous male offspring. It has never been studied in pregnant women, female children, or fetuses. We examined its frequency and cellular nature in normal and diseased female livers from fetal life to adulthood. Forty-six liver samples from 29 women, 6 female children, and 11 female fetuses were screened for the Y chromosome via polymerase chain reaction (PCR) assay and fluorescent in situ hybridization (FISH). The X chromosome was used as an internal control. A third PCR assay was used for Y genotyping. The Y chromosome was detected in 5 of 6 children, 7 of 11 fetuses, 3 of 9 women with normal liver, 7 of 10 women with chronic hepatitis C, 5 of 6 women with acute liver disease during pregnancy with male offspring, and 2 of 4 nonpregnant women with fulminant hepatitis. In positive samples, the mean XY/XX ratio was 0.012 (±0.004). In women, male microchimerism was correlated with previous male offspring. Male hepatocytes, detected via FISH combined with anti-hepatocyte immunohistochemistry, were observed only in fetuses (4/9) and in postpartem women (4/6). Y genotypes were different from each other in 4 of 5 female livers. In conclusion , male liver microchimerism is frequent in normal and diseased female livers. The presence of male cells in the liver of female children and fetuses is probably due to the transplacental transmission of fetal cells preexisting in the mother and acquired either from previous pregnancy with male offspring or during the mother's own fetal life. (Hepatology 2005;42:35–43.)
Diminished dendritic cell (DC) IL-10 production in children with allergic rhinitis (AR) and/or asthma
Authors: R.L. Schreiber, D.A. Gentile, J. Howe-Adams, J. Trecki, A. Patel, P. Fireman, D.P. Skoner
Journal: Journal of Allergy and Clinical Immunology (2003)
High Serum Potassium Concentrations after Recentrifugation of Stored Blood Specimens
Authors: Kenji Hira, Takuro Shimbo, Tsuguya Fukui
Journal: New England Journal of Medicine (2000)

To the Editor: We have encountered several patients who were found to have hyperkalemia only when serum potassium concentrations were measured at a certain community hospital. At that hospital, blood samples were collected from patients into gel-separator tubes and centrifuged 20 to 60 minutes later, but the serum was not removed. Later that day or the next day, the specimens were transported to a commercial laboratory, where they were centrifuged again along with uncentrifuged specimens collected just before transport to the laboratory. We therefore explored the possibility that the handling process caused pseudohyperkalemia. In a time-series study of 5700 specimens . . .
Effect of antiarrhythmic drugs on choline uptake in cardiac cells in culture
Authors: S. W Rabkin
Journal: Cardiovascular Research (1992)

Objective: The aim was to examine the effect on choline uptake of various antiarrhythmic drugs: lignocaine, tocainide, encainide, flecainide, propafenone, procainamide, N-acetylprocainamide, bretylium, and amiodarone. Methods: Cardiac ventricular myocytes from 7 d old chick embryos were exposed in culture to these drugs, at concentrations ranging from 10−6 to 10−4 M, for 24 h. Myocyte choline uptake was assessed by the addition of methyl [3H] choline to media. After 120 min, media were removed, the cells were harvested, and intracellular [3H] actively was counted. Results: Lignocaine and tocainide produced a significant (p<0.05) dose dependent increase in intracellular [methyl 3H] choline. Encainide produced a small increase and flecainide a small decrease in choline, neither of which was dose dependent. Propafenone significantly (p<0.05) altered choline uptake: at 10−6 M and 10−5 M choline uptake was increased and at 10−4 M it was decreased. Amiodarone produced a marked and significant (p<0.05) dose dependent reduction in choline uptake. Bretylium, procainamide, and N-acetylprocainamide did not alter myocyte choline. Conclusions: Choline incorporation into cardiac myocyte is altered by some antiarrhythmic drugs, suggesting this may be part of their antiarrhythmic properties.
Omega-3 Fatty Acids for CHD With Depression—Reply
Authors: Robert M. Carney
Journal: JAMA (2010)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Angiotensin II signal transduction and the mitogen-activated protein kinase pathway
Authors: J. L. Duff, M. B. Marrero, W. G. Paxton, B. Schieffer, K. E. Bernstein, B. C. Berk
Journal: Cardiovascular Research (1995)

In this review, the angiotensin-II-mediated signal transduction pathways involved in vascular smooth muscle cell growth are discussed. Classical pathways involving phospholipase C and protein kinase C, as well as the mitogen-activated protein kinase pathway, are common signal transduction pathways activated by a variety of growth factors to stimulate cell growth. Besides its vasoconstrictor activity, angiotensin II stimulates hypertrophy of vascular smooth muscle cells and is involved in neointimal proliferation following balloon angioplasty. Understanding angiotensin-II-stimulated signaling events, as well as the crosstalk among signaling pathways, may form the basis for the development of new therapies for hypertension and restenosis.
A base mutation of the C-erbA beta thyroid hormone receptor in a kindred with generalized thyroid hormone resistance. Molecular heterogeneity in two other kindreds.
Authors: S J Usala, G E Tennyson, A E Bale, R W Lash, N Gesundheit, F E Wondisford, D Accili, P Hauser, B D Weintraub
Journal: Journal of Clinical Investigation (1990)

Generalized thyroid hormone resistance (GTHR) is a disorder of thyroid hormone action that we have previously shown to be tightly linked to one of the two thyroid hormone receptor genes, c-erbA beta, in a single kindred, A. We now show that in two other kindreds, B and D, with differing phenotypes, there is also linkage between c-erbA beta and GTHR. The combined maximum logarithm of the odds score for all three kindreds at a recombination fraction of 0 was 5.77. In vivo studies had shown a triiodothyronine (T3)-binding affinity abnormality in nuclear receptors of kindred A, and we therefore investigated the defect in c-erbA beta in this kindred by sequencing a major portion of the T3-binding domain in the 3'-region of fibroblast c-erbA beta cDNA and leukocyte c-erbA beta genomic DNA. A base substitution, cytosine to adenine, was found at cDNA position 1643 which altered the proline codon at position 448 to a histidine. By allelic-specific hybridization, this base substitution was found in only one allele of seven affected members, and not found in 10 unaffected members of kindred A, as expected for a dominant disease. Also, this altered base was not found in kindreds B or D, or in 92 random c-erbA beta alleles. These results and the fact that the mutation is predicted to alter the secondary structure of the crucial T3-binding domain of the c-erbA beta receptor suggest this mutation is an excellent candidate for the genetic cause of GTHR in kindred A. Different mutations in the c-erbA beta gene are likely responsible for the variant phenotypes of thyroid hormone resistance in kindreds B and D.
Levels of toxic metals in marine organisms collected from Southern California coastal waters.
Authors: B A Fowler, R C Fay, R L Walter, R D Willis, W F Gutknecht
Journal: Environmental Health Perspectives (1975)

Emission of toxic trace metals into southern California coastal waters has resulted in the extensive accumulation of the elements within marine sediments. The current study was undertaken to evaluate concentrations of trace metals in bottom-dwelling marine fauna collected from two sampling areas. Analyses carried out on muscle samples of the dover sole (Microstomus pacificus) and the crab (Cancer anthonyi) by proton-induced x-ray emission analysis showed considerable concentrations of arsenic and selenium. Samples of gonads, digestive gland, and muscle from the crab Mursia gaudichaudii analyzed by atomic absorption spectroscopy showed elemental concentrations in muscle similar to the crab Cancer anthonyi and much higher metal levels in gonad and digestive gland. These findings suggest the need for further studies concerning the relationship between emission of metals into the marine environment and their abundance in marine fauna.
Accidental percutaneous hexachlorophane intoxication in children.
Authors: F Goutieres, J Aicardi
Journal: BMJ (1977)

Eighteen children with normal skin were accidentally intoxicated by a talc powder containing 6% hexachlorophane. Four died and two remained paraplegic. The clinical picture was intracranial hypertension, eight patients developing signs of spinal cord damage. The condition seemed to result from massive intramyelinic oedema. In the spinal cord vascular disturbances may occur as mechanical complications of oedema, giving rise to permanent sequelae.
Left Ventricular Hypertrophy Regression and Atrial Fibrillation Incidence—Reply
Authors: Peter M. Okin, Richard B. Devereux
Journal: JAMA (2007)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
RT-13 * OPTIMIZING RADIATION THERAPY FOR GLIOBLASTOMA PATIENTS: A COMPARATIVE STUDY OF USING DIFFERENT MRI MODALITIES TO MINIMIZE RADIATION INJURY
Authors: A. Kim, A. W. Lee, A. Lee, T. Yanagihara, A. Jani, S. R. Isaacson, S. K. Cheng, J. N. Bruce, M. B. Sisti, G. M. McKhann, F. Iwamoto, A. Lassman, T. J. C. Wang
Journal: Neuro-Oncology (2014)

Glioblastoma multiforme is the most common and lethal primary malignant brain tumor and radiation therapy is considered the standard of care in the adjuvant setting. Current radiation treatment planning guidelines recommend FLAIR MRI sequence with a 2 cm margin to encompass the subclinical tumor spread. However, the FLAIR modality extensively visualizes the surrounding edema, possibly leading to unnecessary radiation toxicity to healthy brain tissue. We hypothesize that we can optimize radiation therapy by using alternative MRI modalities or by decreasing clinical tumor volume margins to minimize toxicity without compromising accurate tumor targeting. We retrospectively collected data for 21 patients with pathology confirmed recurrence and created radiation treatment plans using ADC, ADC without FLAIR shine-through (ADCst), DWI, T1, and FLAIR. For the FLAIR both a 1 cm and 2 cm margin was used (FLAIR1 and FLAIR2). Boolean operators were used to calculate the accuracy of targeting tumor recurrence and excessive radiation volume compared to the standard FLAIR2 treatment plan. All MRI modalities had complete coverage of the recurrent tumor and the mean differences in accuracy between the different MRI modalities and FLAIR2 was not significant. However, there was a significant reduction in the excessive radiation volume compared to FLAIR2. ADCst had a 51.3% reduction, DWI 42.3%, T1 42.6%, and FLAIR1 44.6% reduction of excessive radiation volume compared to FLAIR2 (p < 0.05). ADC did not have a significant reduction of excessive radiation volume compared to FLAIR2. Our data support the hypothesis that using MRI modalities other than the standard FLAIR or decreasing the margin by 1cm may optimize radiation therapy for GBM patients by reducing unnecessary radiation dose to healthy brain tissue without compromising accuracy. By using new MRI modalities in radiation treatment planning or modifying clinical tumor volume margins we can decrease radiation toxicity to patients to improve their quality of life.
Risk factors for complications of ERCP in primary sclerosing cholangitis
Authors: S. Ismail, L. Kylänpää, H. Mustonen, J. Halttunen, O. Lindström, K. Jokelainen, M. Udd, M. Färkkilä
Journal: Endoscopy (2012)

<b>Background and study aims:</b> Endoscopic retrograde cholangiographic pancreatography (ERCP) is the most accurate technique for surveillance of patients with primary sclerosing cholangitis (PSC). Our aim was to evaluate risk factors for complications of ERCP in patients with PSC. <b>Patients and methods:</b> In 2007 – 2009 we performed 441 ERCPs in patients with PSC. The primary tools for ERCP were a guide wire and papillotomy knife to gain access into the biliary duct. If the primary cannulation failed, and the wire went only into the pancreatic duct, pancreatic sphincterotomy was performed. If necessary, a further oblique cut with a needle knife was done in order to expose the biliary duct. <b>Results:</b> Primary cannulation was successful in 389 patients (88.2 %). Of these, 147 (37.8 %) had had biliary sphincterotomy performed previously. In the group with failed primary cannulation, access into the biliary duct was achieved after pancreatic sphincterotomy in 52 patients. In 11 of these, a further cut with a needle knife was performed. Post-ERCP pancreatitis (PEP) was diagnosed in 31 patients (7.0 %). Factors predicting PEP were female sex (odds ratio [OR] 2.6, <i>P</i> = 0.015) and a guide wire in the pancreatic duct (OR 8.2, <i>P</i> < 0.01). Previous biliary sphincterotomy was a protective factor (OR 0.28, <i>P</i> = 0.02). The risk of PEP increased with the number of times the wire accidentally passed into the pancreatic duct (<i>P</i> < 0.001). Cholangitis developed in 6 patients (1.4 %). <b>Conclusions:</b> In patients with PSC the incidence of ERCP complications remained relatively low. The complication risk increased with the complexity of cannulation. In a patient with PSC in whom follow-up ERCP is planned, biliary sphincterotomy should be considered, as it may protect against PEP.