tbuckley/cabot-search huggingface dataset:
Human T cell gamma genes are frequently rearranged in B-lineage acute lymphoblastic leukemias but not in chronic B cell proliferations.
Authors: Z Chen, D Le Paslier, J Dausset, L Degos, G Flandrin, D Cohen, F Sigaux
Journal: The Journal of Experimental Medicine (1987)

T cell rearranging gene gamma (TRG gamma) and T cell antigen receptor beta (TCR beta) chain gene rearrangement and transcription were studied in a series of patients with B-lineage acute lymphoblastic leukemia (ALL), in which the Ig H chain genes are rearranged and the surface phenotype reproduces the stages of normal pre-B maturation. For comparison, polyclonal T cells from peripheral blood of healthy donors and blast cells from 19 cases of T lineage ALL were also studied. In this study we demonstrate the presence of a clonal rearrangement of the TRG gamma in 18 of the 22 B-lineage ALL cases and establish that this rearrangement, which generally involves the J gamma 1 region, is often monoallelic and appears different from the biallelic J gamma 2 rearrangement frequently seen in T-cell ALLs. In 9 of 22 cases, we found rearrangement of the genes of the TCR beta chain, which never involved the J beta 1 region. Conversely, the TRG gamma were seen in germline configuration in all 19 cases of B chronic lymphoid malignancies. In none of the 9 AML cases studied was TRG gamma and TCR beta chain gene rearrangement found. The TCR beta chain genes were rearranged in one B cell chronic lymphocytic leukemia (CLL). We also show that in B-lineage ALL, the cells probably use the same V gamma genes for TRG gamma rearrangements as the malignant cells in T-ALL and the polyclonal T cells. In none of the 13 B-lineage ALL cases investigated by Northern analysis was TCR beta mRNA expression detected, whereas a weak expression of TRG gamma transcripts was found in two of these cases. The correlations between surface phenotype, rearrangement of TRG gamma, TCR beta, and Ig H chain genes were analyzed. The significance of rearrangement of TRG gamma and TCR beta chain genes in B or pre-B cells is also discussed.
Comparison of the Biologic Activity of Porcine and Semisynthetic Human Insulins Using the Glucose-controlled Insulin Infusion System in Insulin-dependent Diabetes
Authors: Agnes Szekeres, Beverly Worcester, Michael S Ascher, Dee Tuxen, Richard Heyendal, Kathleen M Walsh, M Arthur Charles
Journal: Diabetes Care (1983)

Semisynthetic human insulin is prepared from porcine pancreas by chemical methods involving the substitution of porcine insulin B-30 alanine with threonine. To compare the effectiveness of porcine and semisynthetic human insulins, eight insulin-dependent diabeticpatients were evaluated during two separate periods using a glucose-controlled insulin infusion system. During each 36-h period, patients received either porcine or semisynthetichuman insulin. Patients ingested mixed meals. The mean daily insulin requirements for porcine and semisynthetic human insulins were 84 ± 9 U and 85 ± 6 U (± SEM), respectively (P = NS). Mean blood glucose values were similar at 95 ±1 mg/dl for porcine and 101 ± 3 mg/dl with semisynthetic human insulin (P = NS). Prior metabolic control or insulin antibody levels did not correlate with intravenous insulin requirements. These studies indicate that semisynthetic human insulin is as effective as porcine insulin in maintaining near-normal blood glucose control in short-term intravenous studies using artificial pancreas techniques in insulin-dependent diabetes.
Assessment of myocardial viability in patients with left ventricular dysfunction using contrast-enhanced magnetic resonance imaging: Comparison to 201-Thallium single photon emission computed tomography
Authors: Matthias Regenfus, Christian Schlundt, Johannes von Erffa, Robert Krähner, Dieter Ropers, Stephan Achenbach, Michaela Schmidt, Niels Oesingmann, Kristin Schmiedehausen, Günther Platsch, Torsten Kuwert, Werner G. Daniel
Journal: Journal of the American College of Cardiology (2003)
Abstract 684: Trifluoperazine modulates DNA damage-induced cell death in human lung cancer cells through inhibition of DNA repair, cell cycle delays, and augmentation of pro-apoptotic signaling
Authors: Dali Zong, Petra Hååg, Ihor Yakymovych, Rolf Lewensohn, Kristina Viktorsson
Journal: Cancer Research (2010)

Abstract Trifluoperazine modulates DNA damage-induced cell death in human lung cancer cells through inhibition of DNA repair, cell cycle delays, and augmentation of pro-apoptotic signaling Resistance of human tumors to DNA damaging chemotherapy poses severe limitations to the efficacy of current available treatment. Small molecule compounds of the phenothiazine class, such as trifluoperazine (TFP), have documented chemosensitizing activities. We exposed human non-small cell lung cancer (NSCLC) cell lines to the DNA damaging drug bleomycin in the presence or absence of TFP, and studied potential differences with regard to DNA repair and cell death signaling. A colony formation assay showed that TFP significantly enhanced the cytotoxic potential of bleomycin, at doses achievable clinically. Bleomycin treatment resulted in a reversible arrest at the G2-M phase of the cell cycle. Notably, TFP delayed the repair of bleomycin-induced DNA double strand breaks (DSBs), causing higher residual levels of γH2AX 24 h after treatment. Despite the presence of unrepaired DNA DSBs, cells co-treated with bleomycin and TFP overcame the G2-M checkpoint arrest, as evidenced by the reappearance of the mitosis marker histone H3 phospho-Ser10 and reduction in CFSE fluorescence. This process, which is reminiscent of checkpoint adaptation, was associated with increased micronucleation and secondary arrest in G2-M phase during subsequent rounds of the cell cycle. Furthermore, the onset of secondary checkpoint arrest in cells co-treated with bleomycin and TFP coincided with the activation of pro-apoptotic Bak and Bax, and was followed by activation of caspase-3, which occurred preferentially in cells in the G2-M phase. In conclusion, we demonstrate here that the chemosensitizing effect of TFP in NSCLC cells involves interference with DNA DSBs repair, resulting in secondary G-M checkpoint arrest after initial checkpoint adaptation, and concomitant increases in pro-apoptotic signaling. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 684.
Serum Ctla-4 In Hymenoptera Venom Allergy And Its Modulation By Specific Immunotherapy
Authors: D. Saverino, A. Riccio, A. Rogkakou, M. Bagnasco, P. Bonadonna, R. Simone, A. Chiappori, C. Gamalero, G. Passalacqua, G.W. Canonica
Journal: Journal of Allergy and Clinical Immunology (2009)
Angioscopy and OCT in Repeated In-Stent Restenosis in Saphenous Vein Graft
Authors: Tetsuya Fusazaki, Tomonori Itoh, Tatsuhiko Koeda, Takumi Kimura, Yoshinobu Ogino, Hiroki Matsui, Shoma Sugawara, Motoyuki Nakamura
Journal: JACC. Cardiovascular imaging (2010)
Abortion Law in the Supreme Court
Authors: William J. Curran
Journal: New England Journal of Medicine (1971)

"The subject of abortions — like cases involving obscenity — is one of the most inflammatory ones to reach the Court. People instantly take sides and the public, from which juries are drawn, makes up its mind one way or the other before the case is even argued." [Justice William O. Douglas]ON April 21, 1971, the Supreme Court of the United States joined the abortion law debate. The "treacherous ground" has been entered, and the Justices have begun to speak about morality, health and the preservation of life. (The words are from Justice Jackson,1 who in dissent, warned of . . .
A Decision Analysis of Mandatory Compared with Voluntary HIV Testing in Pregnant Women
Authors: Inaam A. Nakchbandi, J. Craig Longenecker, M. Ann Ricksecker, Richard A. Latta, Cheryl Healton, David G. Smith
Journal: Annals of Internal Medicine (1998)

Background: The benefit of antiretroviral therapy in reducing maternal-fetal transmission of HIV during pregnancy has caused a public policy debate about the relative benefits of mandatory HIV screening and voluntary HIV screening in pregnant women. Objective: To evaluate the benefits and risks of mandatory compared with voluntary HIV testing of pregnant women to help guide research and policy. Design: A decision analysis that incorporated the following variables: acceptance and benefit of prenatal care, acceptance and benefit of zidovudine therapy in HIV-infected women, prevalence of HIV infection, and mandatory compared with voluntary HIV testing. Measurements: The threshold deterrence rate (defined as the percentage of women who, if deterred from seeking prenatal care because of a mandatory HIV testing policy, would offset the benefit of zidovudine in reducing vertical HIV transmission) and the difference between a policy of mandatory testing and a policy of voluntary testing in the absolute number of HIV-infected infants or dead infants. Results: Voluntary HIV testing was preferred over a broad range of values in the model. At baseline, the threshold deterrence rate was 0.4%. At a deterrence rate of 0.5%, the number of infants (n = 167) spared HIV infection annually in the United States under a mandatory HIV testing policy would be lower than the number of perinatal deaths (n = 189) caused by lack of prenatal care. Conclusions: The most important variables in the model were voluntary HIV testing, the deterrence rate associated with mandatory testing compared with voluntary testing, and the prevalence of HIV infection in women of childbearing age. At high levels of acceptance of voluntary HIV testing, the benefits of a policy of mandatory testing are minimal and may create the potential harms of avoiding prenatal care to avoid mandatory testing.
Severe alport phenotype in a woman with two missense mutations in the same COL4A5 gene and preponderant inactivation of the X chromosome carrying the normal allele.
Authors: C Guo, B Van Damme, Y Vanrenterghem, K Devriendt, J J Cassiman, P Marynen
Journal: Journal of Clinical Investigation (1995)

The X-linked form of Alport disease, caused by mutations in the COL4A5 or the COL4A6 gene, usually leads to terminal renal failure in males, while affected females have a more variable and moderate phenotype. We detected in a female patient, with a severe Alport phenotype, two new missense mutations. One mutation (G289V) occurred in exon 15 and converted a glycine in a collagenous domain of COL4A5 to a valine. The second mutation, located in exon 46, substituted a cysteine proximal to the NC1 domain of COL4A5 for an arginine. In white blood cells and kidney both mutations were present on > 90% of the mRNA, while at the genomic level the patient was heterozygous for both mutations. The two mutations therefore occurred in the same COL4A5 allele. No mutation was found in the COL4A5 promoter region by sequencing nor was a major rearrangement of the normal allele detected. A skewed pattern of X inactivation was demonstrated in DNA isolated from the patient's kidney and white blood cells: > 90% of the X chromosomes with the normal COL4A5 allele was inactivated. It is suggested that this skewed inactivation pattern is responsible for the absence of detectable normal COL4A5 mRNA and hence the severe phenotype in this woman.
Jordans' anomaly in a new neutral lipid storage disease
Authors: Elisa Piva, Rachele Pajola, Gianni Binotto, Mario Plebani
Journal: American Journal of Hematology (2008)

Image.Peripheral blood smear: neutrophil (A) eosinophil (B) and monocyte (C) showing lipid vacuoles (Jordans' anomaly) (May Grunwald Giemsa stain, x1000).Same smear, SBB stain: neutrophils (D) with scattered granules and positive reaction in lipid droplets.The arrows indicate two cytoplasmic areas of 1 mm of diameter, similarly to the neutrophil vacuoles seen in the MGG stain.
E. Ratcliffe Anderson, MD, Is New EVP of AMA
Authors: Phil Gunby
Journal: JAMA (1998)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Value of a new screening test for respiratory allergy
Authors: J. Duc, R. Peitrequin, A. Pécoud
Journal: Allergy (1988)

Phadiatop (Pharmacia) is a new in vitro screening test for respiratory allergy which detects in serum the presence of specific IgE to a mixture of common inhalant allergens; results are either positive or negative. Among 100 patients suffering from rhinitis and/or asthma, investigated with 15 skin prick tests (SPT) and RAST for frequent allergens, the Phadiatop was positive in 63: all 63 had at least one positive SPT and RAST (specificity: 100%). Conversely, ther were 67 patients who had a positive RAST for at least one allergen: among these 67 the total IgE concentration in serum was high (greater than 100 kU/l) in 43 (64%) and the Phadiatop was positive in 63 (94%). Phadiatop was negative in serum obtained from 10 cord bloods and 10 non-allergic subjects (disease-free, 15 negative SPT and RAST). Results of the radio-immunoassay originally proposed by the manufacturer were similar to those obtained with a modified enzymo assay using a fluorometric substrate. In the prediction of respiratory allergy, Phadiatop was found to be specific and more accurate than the determination of total IgE in serum.
The Convergent Development of Molecular-Targeted Drugs for Cancer Treatment and Prevention
Authors: Scott M. Lippman, John V. Heymach
Journal: Clinical Cancer Research (2007)

Abstract Advances in our understanding of multistep and field carcinogenesis are erasing the clear demarcation of intraepithelial neoplasia from invasive neoplasia. The growing ability to define a very high risk of cancer is forging important commonalities between prevention and therapy, such as in potential prognostic/predictive markers, agents, and side effects that patients would be willing to tolerate, and the logistics of definitive trials. The emergence of promising new molecular-targeted agents and new technologies for screening and early detection provides new opportunities for applying clinical trial designs that integrate therapy and prevention end points. Such trials may be used to facilitate targeted drug development and help identify strategies for both cancer prevention and advanced cancer therapy. These several advances are creating a convergence of cancer therapy with cancer prevention that promises to streamline the development of targeted drugs and improve the control of major cancers.
Methadone Maintenance in Primary Care
Authors: David A. Fiellin, Patrick G. O'Connor, Marek Chawarski, Juliana P. Pakes, Michael V. Pantalon, Richard S. Schottenfeld
Journal: JAMA (2001)

ContextMethadone maintenance is an effective treatment for opioid dependence, yet its use is restricted to federally licensed narcotic treatment programs (NTPs). Office-based care of stabilized methadone maintenance patients is a promising alternative but no data are available from controlled trials regarding this type of program.ObjectiveTo determine the feasibility and efficacy of office-based methadone maintenance by primary care physicians vs in an NTP for stable opioid-dependent patients.DesignSix-month, randomized controlled open clinical trial conducted February 1999-March 2000.SettingOffices of 6 primary care internists and an NTP.PatientsForty-seven opioid-dependent patients who had been receiving methadone maintenance therapy in an NTP without evidence of illicit drug use for 1 year and without significant untreated psychiatric comorbidity were randomized; 1 patient refused to participate after treatment assignment to NTP.InterventionsPatients were randomly assigned to receive office-based methadone maintenance from primary care physicians, who received specialized training in the care of opioid-dependent patients (n = 22), or usual care at an NTP (n = 24).Main Outcome MeasuresIllicit drug use, clinical instability (persistent drug use), patient and clinician satisfaction, functional status, and use of health, legal, and social services, compared between the 2 groups.ResultsEleven of 22 (50%; 95% confidence interval [CI], 29%-71%) patients in office-based care compared with 9 of 24 (38%; 95% CI, 21%-57%) of NTP patients had a self-report or urine toxicology test result indicating illicit opiate use (P = .39). Hair toxicology testing detected an additional 2 patients in each treatment group with evidence of illicit drug use, but this did not change the overall findings. Ongoing illicit drug use meeting criteria for clinical instability occurred in 4 of 22 (18%; 95% CI, 7%-39%) patients in office-based care compared with 5 of 24 (21%; 95% CI, 9%-41%) NTP patients (P = .82). Sixteen of the 22 (73%; 95% CI, 54%-92%) office-based patients compared with 3 of the 24 (13%; 95% CI, 0%-26%) NTP patients thought the quality of care was excellent (P = .001). There were no differences over time within or between groups in functional status or use of health, legal, or social services.ConclusionsOur results support the feasibility and efficacy of transferring stable opioid-dependent patients receiving methadone maintenance to primary care physicians' offices for continuing treatment and suggest guidelines for identifying patients and clinical monitoring.
Effect of isotretinoin on carbamazepine pharmacokinetics
Authors: J. R. Marsden
Journal: British Journal of Dermatology (1988)

Journal Article Effect of isotretinoin on carbamazepine pharmacokinetics Get access J. R. Marsden J. R. Marsden Department of Dermatology General Hospital Steelhouse Lane Birmingham 64 6NH U.K. Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 119, Issue 3, 1 September 1988, Pages 403–404, https://doi.org/10.1111/j.1365-2133.1988.tb03236.x Published: 01 September 1988
CaseMaster: The Database Solution for General and Thoracic Surgery Residents
Authors: Roger Wotkyns
Journal: JAMA (1993)

An operative list must be prepared for submission to the American Board of Surgery (ABS) as a requirement for certification. Many residents and computer buffs have prepared packages for this purpose. CaseMaster is one of these packages specifically designed for the Macintosh personal computer.  The accumulation of data concerning a resident's experience is an enormously powerful tool in monitoring performance, not only of the resident but also of the residency program. A properly designed computer program could facilitate this. CaseMaster is designed to record either single or multiple resident operative experience. One of us, Dr Sun, has reservations about the capacity of CaseMaster to accept multiple resident input because of the limited database.  Resident input is user friendly and produces information that can be retrieved in the ABS format. The Resident Review Committee (RRC) and the ABS have recently agreed to change the format of case reporting. The new format
Efficacy and safety of tacrolimus ointment 0·1% vs. betamethasone 17-valerate 0·1% in the treatment of chronic paronychia: an unblinded randomized study. Reply from authors
Authors: D. Rigopoulos, S. Gregoriou, H. Belyayeva, G. Larios, G. Kontochristopoulos, A. Katsambas
Journal: British Journal of Dermatology (2010)

Journal Article Efficacy and safety of tacrolimus ointment 0·1% vs. betamethasone 17‐valerate 0·1% in the treatment of chronic paronychia: an unblinded randomized study. Reply from authors Get access D. Rigopoulos, D. Rigopoulos Department of Dermatology, Andreas Sygros Hospital, University of Athens, Athens, Greece Search for other works by this author on: Oxford Academic Google Scholar S. Gregoriou, S. Gregoriou Department of Dermatology, Andreas Sygros Hospital, University of Athens, Athens, Greece S. Gregoriou. E‐mail: stamgreg@yahoo.gr Search for other works by this author on: Oxford Academic Google Scholar H. Belyayeva, H. Belyayeva Department of Dermatology, Andreas Sygros Hospital, University of Athens, Athens, Greece Search for other works by this author on: Oxford Academic Google Scholar G. Larios, G. Larios Department of Dermatology, Andreas Sygros Hospital, University of Athens, Athens, Greece Search for other works by this author on: Oxford Academic Google Scholar G. Kontochristopoulos, G. Kontochristopoulos Department of Dermatology, Andreas Sygros Hospital, University of Athens, Athens, Greece Search for other works by this author on: Oxford Academic Google Scholar A. Katsambas A. Katsambas Department of Dermatology, Andreas Sygros Hospital, University of Athens, Athens, Greece Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 163, Issue 1, 1 July 2010, Pages 208–209, https://doi.org/10.1111/j.1365-2133.2010.09785.x Published: 01 July 2010
BMA meeting: BMA passes vote of no confidence in health secretary
Authors: N. Hawkes
Journal: BMJ (2013)

Thank you for your interest in spreading the word about The BMJ.NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address. Username * Your Email * Send To * You are going to email the following BMA meeting: BMA passes vote of no confidence in health secretary Your Personal Message CAPTCHA This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Echinococcal Disease of the Lung
Authors: Louis Pierucci, Noel H. Fishman, John J. McKeown
Journal: JAMA (1964)

A CASE OF hydatid disease is presented which represents some unusual aspects of a disease encountered infrequently in this country. Judged by world standards, the pulmonary cyst in this patient was quite large. In addition, the best available serologic tests for echinococcosis were negative in this patient. A short description of the disease, its natural history, symptoms, and treatment are included. <h3>Report of a Case</h3> A 21-year-old Creek sailor was referred to Jefferson Medical College Hospital on Sept 19, 1961, after x-rays revealed a large density in his right hemithorax. A farmer and shepherd in his homeland, he had gone to sea two years previously and for the last year was bothered by intermittent right subscapular pain for which he was unable to receive medical attention. When he reached the port of Philadelphia, his complaint of back pain prompted x-ray examination. He was then referred to this hospital for definitive
On Spinal Epilepsy
Authors: A. M. McAldowie
Journal: BMJ (1878)

THE BRITISA MEDICAL 7OURKAL.[(c.21, 1z878 pa-like body on the extensor surface of the left forearm.The teeth d a tendency to a serrated condition of the lower border of the two upper incisors; the gums were inclined to bleed ; the tongue clean.His appetite was fair; he was thirsty, His bowels were open four or five