tbuckley/cabot-search huggingface dataset:
Interleukin-6 (IL-6) modulates migration and matrix metalloproteinase function in dermal fibroblasts from IL-6KO mice
Authors: L.R. Luckett, R.M. Gallucci
Journal: British Journal of Dermatology (2007)

Summary Background Interleukin-6-deficient (IL-6KO) mice display significantly delayed cutaneous wound healing characterized by decreased re-epithelialization, granulation tissue and wound closure. Dermal fibroblasts are one of the principal cell types found in granulation tissue and mediate numerous processes during healing. Objectives To investigate the effects that IL-6 might have on granulation tissue formation and fibroblast motility. As fibroblast motility is associated with matrix metalloproteinase (MMP) activity, the expression of MMP-2 and the tissue inhibitors of metalloproteinase (TIMP)-1 and -2 were assessed. Methods Punch biopsies (4 mm) were performed in the skin of IL-6KO and C57BL/6 mice. The expression of MMP-2, TIMP-1 and -2 in wound tissue was monitored over time. Cellular infiltration and granulation tissue formation was monitored by subcutaneous implantation of polyvinyl alcohol (PVA) sponges. A free-floating collagen lattice model was also used to investigate the direct effects of IL-6 treatment on isolated IL-6KO fibroblasts. The expression of MMP-2, and the inhibitors TIMP-1 and -2, were assessed via real-time quantitative polymerase chain reaction and enzyme-linked immunosorbent assay. Results IL-6KO wounds showed impaired granulation tissue formation 5 days postwounding and fewer fibroblasts had populated the PVA matrices 7 days after implantation in IL-6KO mice compared with wild-type C57BL/6. The mRNA and protein expression of MMP-2 and TIMP-2 mRNA was increased in IL-6KO mice compared with wild-type mice beyond 1 day postwounding, while the expression of TIMP-1 mRNA was transiently higher in IL-6KO only 3 days postwounding. Treatment of collagen lattices with various concentrations of rmIL-6 again showed a dose-response decrease in mRNA and protein expression of MMP-2 and TIMP-2 protein expression, compared with saline control, while TIMP-1 did not appear to be significantly modulated. Conclusions These results indicate that IL-6 influences the function of fibroblasts in wounds, and one mechanism of this regulation may be through the modulation of MMP-2 and TIMP proteins.
Adolescent With 68Ga DOTATATE–Avid Vertebral Hemangioma Mimicking Metastasis in PET Imaging
Authors: Evangelia Skoura, Alshaima Alshammari, Rizwan Syed, Rakesh Sajjan, Jamshed Bomanji
Journal: Clinical Nuclear Medicine (2015)

68Ga DOTATATE PET/CT is a well-established method in the diagnostic workup of neuroendocrine tumors. We report the case of a 15-year-old adolescent boy with histologically proven appendiceal carcinoid tumor referred for 68Ga DOTATATE PET/CT to identify residual or metastatic disease. PET images showed increased tracer uptake in the body of T4 vertebra. This uptake could be misdiagnosed for bone metastasis, but CT characteristic appearance was in keeping with vertebral hemangioma. Both bone metastasis in carcinoid tumor and bone hemangiomas in adolescents are rare conditions, but the combined metabolic and morphological information on PET/CT can lead to the correct diagnosis.
Simple finger tourniquet.
Authors: A Karev
Journal: BMJ (1973)

BY using metacarpal block anaesthesia many surgical procedures on the finger, such as suture of cut wounds, tip injuries, reconstruction of nail deformities, excision of benign tumours and treatment of scar contractures, may be performed. The application of this simple tourniquet provides the desired bloodless field. After the hand has been cleaned and a sterile operating field obtained, local anaesthetic is injected. The patient’s hand is covered with a sterile rubber glove, and the glove tip of the finger to be operated on is snipped off. By rolling back the glove finger to its base (Fig. I) the finger itself is progressively compressed from tip to base proximally and becomes exsanguinated (Fig. 2). The rolled rubber band acts as a tourniquet throughout the operation and is cut transversely at the end of the procedure. The pressure of the rubber band does not appear to jeopardise the neurovascular bundles, as some 200 routine operations have proven. The technique is contra-indicated in the event of infection of the finger as the squeezing of the blood proximally may cause spread of the infection. The same applies to malignant tumours.
618 Introduction of NS5A mutations enables subgenomic HCV-replicon derived from chimpanzee-infectious HC-J4 isolate to replicate efficiently in HUH-7 cells
Authors: S MAEKAWA, N ENOMOTO, N SAKAMOTO, M KUROSAKI, E UEDA, T KOHASHI, H WATANABE, C CHEN, T YAMASHIRO, Y TANABE
Journal: Hepatology (2003)
Results of a United States and Soviet Union joint project on nervous system effects of microwave radiation.
Authors: C L Mitchell, D I McRee, N J Peterson, H A Tilson, M G Shandala, M I Rudnev, V V Varetskii, M I Navakatikyan
Journal: Environmental Health Perspectives (1989)

During the course of a formal program of cooperation between the United States and the Soviet Union concerning the biological effects of physical factors in the environment, it was concluded that duplicate projects should be initiated with the general goal of determining the most sensitive and valid test procedures for evaluating the effects of microwave radiation on the central nervous system. This report details an initial step in this direction. Male rats of the Fischer 344 strain were exposed or sham exposed to 10 mW/cm2 continuous wave microwave radiation at 2.45 GHz for a period of 7 hr. Animals were subjected to behavioral, biochemical, or electrophysiological measurements during and/or immediately after exposure. Behavioral tests used were passive avoidance and activity in an open field. Biochemical measurements were ATPase (Na+, K+; Mg2+, Ca2+) and K+ alkaline phosphatase activities. Electrophysiological measurements consisted of EEG frequency analysis. Neither group observed a significant effect of microwave irradiation on open field activity. Both groups observed changes in variability of the data obtained using the passive avoidance procedure, but not in the same parameters. The U.S. group, but not the USSR group, found significantly less Na+,K+-ATPase activity in the microwave-exposed animals compared to the sham exposed animals. Both groups found incidences of statistically significant effects in the power spectral analysis of EEG frequency, but not at the same frequency. The failure of both groups to substantiate the results of the other reinforces our contention that such duplicate projects are important and necessary.
Preliminary report: pharmacologic 11β-hydroxysteroid dehydrogenase type 1 inhibition increases hepatic fat oxidation in vivo and expression of related genes in rats fed an obesogenic diet
Authors: Magalie Berthiaume, Mathieu Laplante, William T. Festuccia, Joel P. Berger, Rolf Thieringer, Yves Deshaies
Journal: Metabolism (2009)
BACTERIOLOGIC AND BLOOD FINDINGS IN THIRTY-SIX CASES OF BOWEL INFECTION OF INFANTS.
Authors: J. C. COOK
Journal: JAMA (1906)

The 36 cases which I am about to present have been selected from 350 cases of diseases of children, which came under my supervision in the Jackson Park Sanitarium during the summer of 1905. Those cases, the clinical aspect of which showed more or less severe type of anemia, are the ones selected for the special work to which I wish to call your attention. The work was taken up with the hope of obtaining findings pointing to the relation of the condition of the blood, the enlargement of the glands and spleen and the bacterial and metabolic intoxications. The most important data obtained in each case are incorporated in the table. The cases have been arranged with regard to the red blood count, the highest to the lowest, that being the most logical order on which to make comparisons. In every case of examination of the blood, including a
A Foreign Body in the Kidney
Authors: D. Macaulay, T. Moore
Journal: BMJ (1955)

The clinical and immunological aspects of 16 children with the syndrome of hypogammaglobulinaemia associated with normal or increased IgM (the hyper IgM syndrome) and their responses to treatment are reviewed. Increased concentrations of IgM, neutropenia, and recurrent infections could usually be controlled by antimicrobial and intravenous immunoglobulin treatment. Together with the bacterial infections characteristic of hypogammaglobulinaemia, these patients often developed opportunistic infections, including Pneumocystis carinii pneumonia, often presenting in the first year of life. The occurrence of sclerosing cholangitis, neurological complications, and neutropenia may be a result of an underlying cell mediated immune deficiency, autoimmunity, or infection. Despite a high incidence of opportunistic infections, immunological investigations did not show any abnormality of T cell function. These findings are discussed in the light of the recent demonstration that the lack of expression of a T lymphocyte activation antigen is the molecular basis of the X linked form of the disorder.
Inflammatory cytokines up-regulate intercellular adhesion molecule-1 expression on primary cultured mouse hepatocytes and T-lymphocyte adhesion
Authors: Masahiko Morita, Yoshifumi Watanabe, Toshihiro Akaike
Journal: Hepatology (1994)

We investigated the effect of inflammatory cytokines on the intercellular adhesion molecule-1 expression on primary cultured murine hepatocytes. Tumor necrosis factor-alpha, interferon-gamma and interleukin-1 alpha up-regulated the intercellular adhesion molecule-1 expression on hepatocytes in a dose-dependent fashion; however, interleukin-6 did not. On the basis of kinetic analysis, the expression level reached a peak 24 hr after stimulation, and both cycloheximide and actinomycin D inhibited the expression. Furthermore, T lymphocytes bind more to interferon-gamma-stimulated hepatocytes than to unstimulated hepatocytes. The binding was dependent on the concentration of interferon-gamma. The binding was also up-regulated by stimulating T lymphocytes with phorbol myristate acetate. Tumor necrosis factor-alpha and interleukin-1 alpha demonstrated the same effect as interferon-gamma, whereas interleukin-6 did not increase T-lymphocyte adhesion to the hepatocytes. The adhesion induced by interferon-gamma or tumor necrosis factor-alpha was inhibited by antibody against either intercellular adhesion molecule-1 or lymphocyte function-associated antigen-1, a ligand for intercellular adhesion molecule-1, but was not inhibited by CD44 antibodies. These results demonstrate that inflammatory lymphokines enhance the T-lymphocyte adhesion to primary cultured hepatocytes by up-regulating the intercellular adhesion molecule-1 expression on the stimulated hepatocytes by activating the de novo pathway. This mechanism may play an important role in the pathogenesis of hepatitis.
Chronic Fatigue Syndrome Among Adolescents
Authors: Megan Moreno
Journal: JAMA Pediatrics (2014)

Dag Sulheim, MD; Even Fagermoen, MD; Anette Winger, RN, MA; Anders Mikal Andersen, BSc; Kristin Godang, BSc; Fredrik Müller, MD, PhD; Peter C. Rowe, MD, PhD; J. Philip Saul, MD; Eva Skovlund, PhD; Merete Glenne Øie, PhD; Vegard Bruun Wyller, MD, PhD
Priorities to Improve Health Outcomes
Authors: John Kvasnicka
Journal: JAMA (2007)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Effects of Agent Orange exposure
Authors: F. DeStefano
Journal: JAMA (1995)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Cardiovascular and Subjective Responses to Inhalation of Carbon Dioxide
Authors: M.A. van den Hout, E. Griez
Journal: Psychotherapy and Psychosomatics (1982)

Cardiovascular and subjective responses to inhalation of a 35% CO2 mixture were studied in a sample of anxious neurotics. Inhalation of air was used as a placebo control treatment. In comparison with the inhalation of air, CO2 produced a significant drop in diastolic blood pressure. A drop in heart rate frequency was observed both after inhalation of CO2 and air. CO2 inhalation did not produce an average decline in subjective feelings of distress. Explanations of these data are offered and they are compared with earlier findings. Some speculations are made on the clinical usefulness of CO2 inhalation in the treatment of anxious neurotics.
The Metric System
Authors: G. Mouchot
Journal: BMJ (1953)

<b>Objective</b>&nbsp;To examine whether dietary intake of specific flavonoid subclasses (including flavonols, flavones, flavanones, flavan-3-ols, anthocyanins, and flavonoid polymers) is associated with weight change over time. <b>Design</b>&nbsp;Three prospective cohort studies. <b>Setting</b>&nbsp;Health professionals in the United States. <b>Participants</b>&nbsp;124 086 men and women participating in the Health Professionals Follow-up Study (HPFS), Nurses’ Health Study (NHS), and Nurses’ Health Study II (NHS II). <b>Main outcome measure</b>&nbsp;Self reported change in weight over multiple four year time intervals between 1986 and 2011. <b>Results</b>&nbsp;Increased consumption of most flavonoid subclasses, including flavonols, flavan-3-ols, anthocyanins, and flavonoid polymers, was inversely associated with weight change over four year time intervals, after adjustment for simultaneous changes in other lifestyle factors including other aspects of diet, smoking status, and physical activity. In the pooled results, the greatest magnitude of association was observed for anthocyanins (−0.23 (95% confidence interval −0.30 to −0.15) lbs per additional standard deviation/day, 10 mg), flavonoid polymers (−0.18 (−0.28 to −0.08) lbs per additional SD/day, 138 mg), and flavonols (−0.16 (−0.26 to −0.06) lbs per additional SD/day, 7 mg). After additional adjustment for fiber intake, associations remained significant for anthocyanins, proanthocyanidins, and total flavonoid polymers but were attenuated and no longer statistically significant for other subclasses. <b>Conclusions</b>&nbsp;Higher intake of foods rich in flavonols, flavan-3-ols, anthocyanins, and flavonoid polymers may contribute to weight maintenance in adulthood and may help to refine dietary recommendations for the prevention of obesity and its potential consequences.
Sponsors meet scientists to speed pediatric medicines development
Authors: Jonathan M. Davis, William E. Smoyer, Edward M. Connor, W. Charles Huskins, Cindy Pastern, Mary Purucker, Elisabeth C. Schrader, Cynthia R. Jackson, Frederick J. Kaskel, Steven Hirschfeld
Journal: Science Translational Medicine (2015)

Child-health research initiative enables rapid response to opportunities for participation in multicenter pediatric clinical trials.
Reversible "Fanconi Syndrome" Caused by Degraded Tetracycline
Authors: George W. Frimpter
Journal: JAMA (1963)

A syndrome of nausea, vomiting, proteinuria, acidosis, glycosuria, and aminoaciduria was observed in three patients. The clinical picture at first resembled that of diabetes mellitus. The three patients, a 54-yr-old woman, a 13-yr-old girl, and a 13-yr-old boy, were found to have taken deteriorated preparations of tetracycline as treatment, respectively, for maxillary sinusitis, pharyngitis, and respiratory infection. The two children had received tetracycline on previous occasions without bad effects. All three patients recovered in about a month. A degradation product of tetracycline such as epi-anhydrotetracycline or anhydrotetracycline was probably the etiologic factor.
Abstract 2361: SNP (-617G&amp;gt;A) in ARE-like loci of the <i>NRF2</i> gene: a new biomarker for prognosis of lung adenocarcinoma.
Authors: Yasuko Okano, Yasushi Ichikawa, Yohei Miyagi, Yutaka Natsumeda, Toshihisa Ishikawa
Journal: Cancer Research (2013)

Abstract Purpose: Accumulating evidence suggests nuclear factor (erythroid-derived 2)-like 2 (NRF2) plays an important role as cellular defense, tumor suppression, and oncogenesis. However, little information is available as to the genetic polymorohisms of the NRF2 gene and their clinical relevance. We aimed to validate the NRF2 gene as a prognostic biomarker in lung cancer. Experimental Design: We prepared genomic DNA from 392 Japanese patients with primary lung cancer and detected a SNP (c.-617C&amp;gt;A) in the ARE-like loci of the human NRF2 gene by the rapid genetic testing method we developed in this study. We analyzed an association between the NRF2 gene and overall survival. Results: We detected homozygous (A/A) and heterozygous (C/A) alleles with the SNP (c.-617C&amp;gt;A) in 24 (6.2%) and 147 (38.0%) patients, respectively. The frequency of the homozygote (A/A) was four-fold higher in females than in males (11.0% versus 2.7%). Multivariate logistic regression models including standard lung cancer factors revealed that the homozygous alleles (c.-617A/A) were significantly related to gender. Interestingly, lung cancer patients carrying the homozygous alleles (c.-617A/A) exhibited remarkable survival over 1,700 days after surgical operation (log-rank p=0.021). Conclusion: The homozygous allele (c.-617A/A) in the NRF2 gene is considered to be a prognostic biomarker for assessing both the gender-related risk of lung adenocarcinoma in the non-smoking sub-population and their overall survival. Citation Format: Yasuko Okano, Yasushi Ichikawa, Yohei Miyagi, Yutaka Natsumeda, Toshihisa Ishikawa. SNP (-617G&amp;gt;A) in ARE-like loci of the NRF2 gene: a new biomarker for prognosis of lung adenocarcinoma. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2361. doi:10.1158/1538-7445.AM2013-2361
Open label trials
Authors: P. Sedgwick
Journal: BMJ (2011)

Researchers assessed the effectiveness of a supervised exercise programme for the treatment of patellofemoral pain syndrome. An open label randomised controlled trial was performed. Participants with a new episode of patellofemoral pain syndrome were recruited by general practitioners or sport physicians. The intervention group received a six week standardised exercise programme tailored to individual performance and supervised by a physical therapist; people in this group were instructed to practise the personalised exercises at home for three months. The control group received usual care, comprising a “wait and see” approach of rest …
Seasonal variation of IgE synthesis in vitro by human peripheral blood mononuclear cells
Authors: B. Björkstén, A. Gamkrelidze, T. Vanto, M. Kjellman
Journal: Allergy (1990)

Seasonal variations in IgE antibody synthesis in vitro were studied in cultures of blood mononuclear cells (MNC) from 11 pollen allergic individuals. The IgE levels were significantly higher in two summer seasons than in the winter and spring between them. Net synthesis was confined to the summer in all but one of the patients. All the IgE in the cultures outside the pollen season represented preformed IgE which was present mainly (59%) in the monocyte fraction. Thus, preformed IgE seems to persist in monocytes at times when there is little de novo synthesis of IgE.
Metabolic alkalosis in the rat. Evidence that reduced glomerular filtration rather than enhanced tubular bicarbonate reabsorption is responsible for maintaining the alkalotic state.
Authors: M G Cogan, F Y Liu
Journal: Journal of Clinical Investigation (1983)

Maintenance of chronic metabolic alkalosis might occur by a reduction in glomerular filtration rate (GFR) without increased bicarbonate reabsorption or, alternatively, by augmentation of bicarbonate reabsorption with a normal GFR. To differentiate these possibilities, free-flow micropuncture was performed in alkalotic Munich-Wistar rats with a glomerular ultrafiltrate total CO2 concentration of 46.5 +/- 0.9 mM (vs. 27.7 +/- 0.9 mM in controls). Alkalotic animals had a markedly reduced single nephron GFR compared with controls (27.4 +/- 1.5 vs. 51.6 +/- 1.6 nl/min) and consequently unchanged filtered load of bicarbonate. Absolute proximal bicarbonate reabsorption in alkalotic animals was similar to controls (981 +/- 49 vs. 1,081 +/- 57 pmol/min), despite a higher luminal bicarbonate concentration, contracted extracellular volume, and potassium depletion. When single nephron GFR during alkalosis was increased toward normal by isohydric volume expansion or in another group by isotonic bicarbonate loading, absolute proximal bicarbonate reabsorption was not substantially augmented and bicarbonaturia developed. To confirm that a fall in GFR occurs during metabolic alkalosis, additional clearance studies were performed. Awake rats were studied before and after induction of metabolic alkalosis associated with varying amounts of potassium and chloride depletion. In all cases, the rise in blood bicarbonate concentration was inversely proportional to a reduction in GFR; filtered bicarbonate load remained normal. In conclusion, a reduction in GFR is proposed as being critical for maintaining chronic metabolic alkalosis in the rat. Constancy of the filtered bicarbonate load allows normal rates of renal bicarbonate reabsorption to maintain the alkalotic state.
German hospital directors accused of bribery
Authors: H L Karcher
Journal: BMJ (1994)

Directors of 50 out of 51 heart centres in Germany have accepted bribes from producers of artificial heart valves for choosing their products, the news magazine Der Spiegel reported last month.

The scandal began when Karl Kaula, director of the Verband der Angestellten- Krankenkassen (the association of employees' health insurances), wrote to the minister of health, …