tbuckley/cabot-search huggingface dataset:
Abstract 4972: Putative mechanism of TAP63α regulation of chondrocyte maturation during long bone development
Authors: Feifei Li, Yaojuan Lu, Sam Abbassi, Ming Ding, Guojun Wu, Siying Wang, Qiping Zheng
Journal: Cancer Research (2010)

Abstract p63 is the mouse homologue of the well-known tumor suppressor P53. While it remains controversial as to whether P63 functions as a tumor suppressor gene or an oncogene, mouse genetic studies and mutation analysis of human SHFM syndrome (Split Hand/Foot Malformation) have clearly demonstrated that P63 plays an important role in skin and limb development. The truncated limbs observed in p63 null mice suggest that P63 may contribute to long bone development by playing a role during endochondral bone formation. However, how P63 regulates target gene expression and is linked to endochondral ossification remains largely unknown. Here we present some preliminary data suggesting that P63 may regulate type X collagen gene (Col10a1) expression, thereby impacting the process of chondrocyte maturation. The type X collagen gene (Col10a1) is specifically expressed in hypertrophic chondrocytes, a critical cell stage linking both skeletal development and multiple skeletal diseases. We have previously shown that a 150-bp Col10a1 cis-enhancer is sufficient to mediate its cell-specific expression in vivo. Our further in vitro studies suggest that Runx2, a master transcription factor for osteoblast differentiation as well as for chondrocyte maturation, is one of the major factors that regulate Col10a1 expression via interaction with its tandem repeat Runx2 binding sites. Interestingly, by yeast one-hybrid approach using this cis-enhancer as bait, we identified p53 related proteins as candidate factors that may contribute to the regulation of Col10a1 expression. We have performed Real-time RT-PCR to examine p63 and Runx2 expression in MCT cells, a cell model that shows significant upregulation of Col10a1 upon growth arrest. Both p63 and Runx2 showed around 2-fold upregulation in hypertrophic MCT cells. These data suggest that P63 may collaborate with Runx2 and together regulate Col10a1 expression during chondrocyte maturation. To further explore the putative function of P63 during skeletal development, we have successfully established transgenic mouse lines in which HA and Flag tagged human TAP63α cDNA (the longest P63 isotype) is driven by previously described hypertrophic chondrocyte-specific Col10a1 control element (Col10a1-TAP63α). By phenotypic analysis of these transgenic mice during different developmental stages, we will be able to define the consequence and the potential mechanism of P63 on skeletal development and skeletal disorders. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4972.
Short-Term Estrogen Administration Ameliorates Dobutamine-Induced Myocardial Ischemia in Postmenopausal Women With Coronary Artery Disease
Authors: Mete Alpaslan, Hiroaki Shimokawa, Mari Kuroiwa-Matsumoto, Yasuhiko Harasawa, Akira Takeshita
Journal: Journal of the American College of Cardiology (1997)
Involvement of fusion activity of ultraviolet light-inactivated sendai virus in formation of target antigens recognized by cytotoxic T cells
Authors: K Sugamura, K Shimizu, FH Back
Journal: The Journal of Experimental Medicine (1978)

Mice inoculated with ultraviolet light-inactivated Sendai virus mount a cell- mediated immune response to the virus. Cytotoxic T cells specific for Sendai virus can be obtained by in vitro secondary stimulation of primed spleen cells with syngeneic stimulator cells coated with UV-inactivated Sendai virus. Neither in vivo nor in vitro stimulation alone is sufficient to generate specific cytotoxic T cells. Sharing of the H-2 haplotype between cytotoxic T cells and target cells is required for the Sendai virus-specific lysis to occur. The fusion (F) glycoprotein of Sendai virus has been implicated in target antigen formation (20). Ethanol treatment of Sendai virus causes complete inactivation of the cell-fusion and hemolytic activities of the envelope, but does not affect the antigenicity of the F glycoprotein; furthermore, hemagglutinin and neuraminidase activities of the envelope HANA glycoprotein are also left intact after ethanol treatment. Target cells can be prepared by coating them with various numbers of UV-inactivated Sendai virus that have been treated with ethanol or, as a control, phosphate-buffered saline (PBS). The amount of virus adsorbed to target cells during the cytotoxicity reaction time using either ethanol-treated or untreated (PBS "treated") virions is essentially identical, but target cells coated with ethanol-treated Sendai virus fail to serve as targets for cytotoxic T cells. These results indicate that fusion activity of the Sendai virus envelope is essential to the formation of the target antigen and that virus adsorption to cell surfaces without fusion of the envelope with cell membranes is not sufficient to allow killing by virus-specific cytotoxic T cells.
An Improved Method of Performing the 'Boutonniere' Operation
Authors: W. F. Teevan
Journal: BMJ (1876)

Although we are thus led to the conclusion that the sounds of the heart originate, not in the blood, but in the solids, it is nevertheless certain that the presence of that fluid, its quantity, and its quality, have ai imuch share in modifying the sounds as they have in in uencing the action of the heart.To this I shall recur; but, to enforce my chief conclusion, I will contrast the difficulty of producing sound in liquids with the readiness with which they are caused by solids under water.The slightest knock, rub, or scratch of one solid against another makes quite a little noise, which the stethoscope transmits from the water to the ear; and the sudden tightening of a tape or of a bit of cloth or mem- brane under water gives a similar result, the sounds in the latter ex- amples often closely resembling those of the heart.The noise of such motions under water is less than in air, because the liquid somewhat deadens the sound; but they are quite distinct, and the more exactly represcut the rounds of the heart, inasmuch as the motions of this organ are enveloped in liquid and wet membrane.The chest-walls, or a stomach distended with gas, sometimes act like a sounding-board to the heart-sounds, and _make them as loud as if they wvere produced in air.I wfill conclude wvith a summary of the most conclusive observations in my experiments on asses in iS35.The animals were poisoned with woora.ra-dead, in fact, except the heart, which, by aid of artificial resipjration, continued to contract vigorously for more than an hour.EXPERIMENT 1.-i.The first sound was equally audible on all parls of the *ventricles, simultaneously with the shock of their sudden tightening in the systole.2. The second sound was most distinct near t'e roots of the large arteries, being audible there in the .weakerpulsa- tiolls, wshen it could not be heard elsewhere. 3Pressure on the arte- risk roots, by the fingers or the stethoscope, invariably stopped the seconld sound.Slight pressure caused a bellows-murmur with the first sound.4. On pushing the auricles by the end of a finger into each auriculo-ventricula-opening, the ventricular contractions became weak andI irregular; but the first sound, although weak, was still heard alone.6.The left auricle was cut open, and the mitral valve par- t;lal!y Gti-eroyed.The blood issued in jets at each systole; yet the first sounld still accoimpanied the systole, but the second sound ceased.The rihlit auricle xt-as freely laid open ; still the first sound continued.S. I pu!lshed my finger through the mitral orifice into the left ventricle, andl pressed on the right so as to prevent the influx of blood into either ventricle.The ventricles continued to contract strongly ; and the first sound was still distinct, but weak.
Traveler's Ankle
Authors: H. Daintree Johnson
Journal: JAMA (1973)

<h3>To the Editor.—</h3> Many people have had to give up long journeys by coach or airplane due to the discomfort of ankle swelling. Passengers in cars and trains appear to be immune, and it would seem to be the unrelieved knee flexion that must be to blame. Similar conditions, as in a crowded air-raid shelter or prolonged tight flexion of knees over the rail of a deck chair, occasionally give rise to deep venous thrombosis (shelter leg, steamer leg). These have often been attributed to imagined kinking of the popliteal vein with obstruction, but no evidence of any such obstruction can be seen when the leg is flexed during phlebography. When blood flow through veins is at a minimum, there must be segments, the contents of which become completely stagnant. This would be particularly apt to happen in the numerous, wide sinusoidal veins of calf muscle, and these are indeed
Pneumococcal Vaccination and Cardiovascular Events in Men—Reply
Authors: Hung Fu Tseng
Journal: JAMA (2010)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
The effect of a polyurethane-based reverse thermal gel on bone marrow stromal cell transplant survival and spinal cord repair
Authors: Gaby J. Ritfeld, Britta M. Rauck, Tabitha L. Novosat, Daewon Park, Pavan Patel, Raymund A.C. Roos, Yadong Wang, Martin Oudega
Journal: Biomaterials (2013)
Diagnostic Value of Bone and Ga-67 Imaging in Skeletal Tuberculosis
Authors: WAN-YU LIN, SHYH-JEN WANG, KAI-YUAN CHENG, YEH-YOU SHEN, SHENG-PING CHANGLAI
Journal: Clinical Nuclear Medicine (1998)

Tuberculosis (TB) remains a major cause of skeletal infection in many parts of the world. Bone scintigraphy is an excellent screening test for bone lesions and Ga-67 scintigraphy is a useful tool for detecting inflammatory lesions. This study determined the value of bone and Ga-67 scans in patients with skeletal TB. Tc-99m MDP and Ga-67 whole-body scans were performed in 24 patients with proved skeletal TB. Twenty-six TB lesions were found in these 24 patients, 8 in the spine, 5 in the knee, 5 in the wrist, 4 in the ankle, 2 in the elbow, 1 in the finger, and 1 in the sacroiliac joint. Of the 26 TB lesions, Tc-99m MDP bone imaging detected 24 and Ga-67 scans revealed 23. The sensitivity rates were 92% (24 of 26) and 88.5% (23 of 26) for bone scans and Ga-67 scans, respectively. When the scans were evaluated in combination, the detection sensitivity was 96.1% (25 of 26). Two lesions that the bone scan failed to detect were in the spine. Ga-67 imaging failed to detect two spinal lesions and one lesion in the knee. The detection sensitivities of bone and gallium scans were high for skeletal TB, although neither scan was specific for TB lesions. Either the bone or Ga-67 scan can be used in the clinical setting as a convenient screening test to detect multiple sites of bone involvement in TB, and to indicate the sites for further detailed evaluation by CT, MRI, or biopsy.
SMOKING AND PHARMACODYNAMIC PROFILING OF ASPIRIN VERSUS CLOPIDOGREL MONOTHERAPY IN PATIENTS WITH ATHEROSCLEROTIC DISEASE
Authors: Fabiana Rollini, Francesco Franchi, Jung Rae Cho, Christopher DeGroat, Mona Bhatti, Elisabetta Ferrante, Antonio Tello-Montoliu, Ronakkumar Patel, Andrew Darlington, Bhaloo Desai, Jose’ Luis Ferreiro, Ana Muniz-Lozano, Martin Zenni, Luis Guzman, Theodore Bass, Dominick Angiolillo
Journal: Journal of the American College of Cardiology (2014)
The effects of triamcinolone acetonide aqueous nasal spray on adrenocortical function in children with allergic rhinitis☆☆☆★★★
Authors: Anjuli S. Nayak, Mark H. Ellis, Gary N. Gross, Louis M. Mendelson, Eric J. Schenkel, Bobby Q. Lanier, Brandon Simpson, Mary Ellen Mullin, Joseph A. Smith
Journal: Journal of Allergy and Clinical Immunology (1998)
Prescription charges: are they worth it?
Authors: J. Appleby
Journal: BMJ (2014)

John Appleby assesses whether the policy of charging for prescriptions in England is helping or harming the NHS

While Wales, Northern Ireland, and Scotland have abandoned charges for prescriptions, in April the English saw prescription charges rise once again, to £8.05 (€10; $13.5) per prescribed item. Labour peer David Lipsey has suggested that the charges are, “a dog’s dinner lacking any basis in fairness or logic and stuffed with anomalies and inconsistencies.”1 But apart from unfairness, illogicality, and anomalies, is there a fundamental problem with such charges?

Although prescription charges have been rising, the latest data show that the number of pharmaceutical items prescribed in England reached its highest level yet—over one billion in 2012—equivalent to nearly 19 per person and an increase of 62% since 2002 (fig 1⇓).2 But as fig 1 also shows, the proportion of prescribed items that attracted a charge was small (around 10%) because …
Intra-arterial Radionuclide Infusion as an Aid to Assess Chemotherapy Perfusion Patterns
Authors: J HUYS, M BORMS, M TROCH
Journal: Clinical Nuclear Medicine (1983)

Regional treatment of advanced head and neck cancer by intra-arterial infusion has been tried for several years. The aim of such treatment is to increase the concentration of the drug in the tumor area while decreasing systemic toxicity. The exact localization of the intra-arterial catheter is thereby the key to effective treatment. This report describes the results of radionuclide imaging as an aid to defining the perfusion pattern in comparison with vital dye injection and contrast angiography. It is concluded that radionuclide infusion imaging is the best method to monitor the localization of the catheter.
Depressed-Type Hyperplastic Lesion in the Colon
Authors: S. Tamura, H. Ueta, T. Miyamoto, H. Mizuta, S. Onishi
Journal: Endoscopy (2004)

A 77-year-old man was referred for evaluation of anal bleeding. He had no history of any cancer and no family history of colorectal cancer. Colonoscopy using a magnifying videoscope (CF240ZI; Olympus, Tokyo, Japan) was performed and an advanced colon cancer at the rectum and a slightly reddish flat lesion at the ascending colon were detected. The depressed lesion in the ascending colon became obvious when sprayed with 0.2 % indigo carmine solution (Figure [1]). The lesion was about 6 mm in diameter, and the clarified margin of depression was well demarcated. The magnified view of the depressed lesion after spraying with 0.2 % indigo carmine showed a type II pit pattern and a normal round pit pattern (type I) along the marginal elevation according to the classification proposed by S. Kudo et al., indicating that this lesion was hyperplastic [1]. The patient underwent an endoscopic mucosal resection [2]. Histological examination revealed the characteristic non-neoplastic proliferation of serrated tubular profiles of a hyperplastic polyp (Figure [2]).
Safety and Efficacy of Endoscopic Ultrasound-Guided Drainage of Pancreatic Fluid Collections With Lumen-Apposing Covered Self-Expanding Metal Stents
Authors: Raj J. Shah, Janak N. Shah, Irving Waxman, Thomas E. Kowalski, Andres Sanchez-Yague, Jose Nieto, Brian C. Brauer, Monica Gaidhane, Michel Kahaleh
Journal: Clinical Gastroenterology and Hepatology (2014)
SIK2 Is a Centrosome Kinase Required for Bipolar Mitotic Spindle Formation that Provides a Potential Target for Therapy in Ovarian Cancer
Authors: Ahmed Ashour Ahmed, Zhen Lu, Nicholas B. Jennings, Dariush Etemadmoghadam, Luisa Capalbo, Rodrigo O. Jacamo, Nuno Barbosa-Morais, Xiao-Feng Le, Pablo Vivas-Mejia, Gabriel Lopez-Berestein, Geoffrey Grandjean, Geoffrey Bartholomeusz, Warren Liao, Michael Andreeff, David Bowtell, David M. Glover, Anil K. Sood, Robert C. Bast
Journal: Cancer Cell (2010)
Erythroplasia vs. Leukoplakia in the Diagnosis of Early Asymptomatic Oral Squamous Carcinoma
Authors: Arthur Mashberg
Journal: New England Journal of Medicine (1977)

Although oral squamous cancer in the United States accounts for approximately 5 per cent of neoplasms in men and 2 per cent in women,1 the teaching of oral-cancer concepts, and especially its diagnosis, remains unsatisfactory. Even though the oral mucosa is easily accessible for visual examination, and squamous carcinoma is readily discernible in its early asymptomatic stages, long before ulceration, bleeding, induration and lymphadenopathy are present, 60 per cent2 of these intraoral lesions are well advanced (at least Stage III-T2 N1 MO)* at the time of discovery. The greatest concern of therapists is with the treatment of the symptomatic lesion. . . .
Essential thrombocythemia terminating in pure erythroleukemia
Authors: Andreas Kreft, Jürgen Burg, Thomas Fischer, Charles James Kirkpatrick
Journal: American Journal of Hematology (2004)

Abstract Transformation into acute leukemia is a rare event in essential thrombocythemia (ET). The blasts are usually of myeloid, rarely of megakaryoblastic differentiation. We present the case of a patient with pure erythroleukemia after a nearly 10‐year course of ET, which was treated with hydroxyurea. The patient, a 58‐year‐old male, presented with an elevated thrombocyte count (926,000/μL) and normal values of hemoglobin and leukocytes. After 10 years of therapy with hydroxyurea, the patient developed acute leukemia of solely erythroid differentiation. Chemotherapy with cytarabine and daunorubicin resulted in incomplete remission. The patient died 2 months after diagnosis of acute erythroleukemia. Transformation of ET into erythroleukemia may demonstrate the pluripotent potential of the neoplastic hemopoietic stem cell, with the ability to cause acute leukemia not only of myeloid or megakaryoblastic but also of erythroid lineage. Am. J. Hematol. 77:140–143, 2004. © 2004 Wiley‐Liss, Inc.
The Little Black Book of Primary Care
Authors: Allison Oler
Journal: Annals of Internal Medicine (1999)

Medical Writings: Book Notes1 June 1999The Little Black Book of Primary CareAllison Oler, MDAllison Oler, MDHospital of the University of Pennsylvania, Philadelphia, Pennsylvania. (Oler)Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-130-11-199906010-00026 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Third edition. Onion DK; ed. 923 pages. Malden, MA: Blackwell Science; 1999. $39.95. ISBN 0865424896. Order phone 800-759-6102.Field of medicine: General internal medicine and family practice.Format: Spiralbound book.Audience: Medical students and practitioners who are new to seeing patients in a primary care setting.Purpose: To act as a starter notebook for students and residents; more experienced clinicians may use it as a source for updating references.Content: The book contains 117 short chapters grouped into 21 sections. The first chapter gives brief synopses of management of emergent situations, including cardiac arrest, poisoning, and overdose. Subsequent chapters focus ... Author, Article, and Disclosure InformationAffiliations: Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania. (Oler) PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 June 1999Volume 130, Issue 11Page: 952KeywordsAlgorithmsComputer softwareDecision makingDisabilitiesEpidemiologyHeartLiver function testsMini mental state examinationOutpatientsToxic shock syndrome ePublished: 15 August 2000 Issue Published: 1 June 1999 Copyright & PermissionsCopyright © 1999 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
High incidence of sputum smear negative tuberculosis during HAART in Burkina Faso
Authors: M. Dembele, N. Saleri, G. B. Migliori, H. Ouedraogo, A. C. C. Carvalho, M. Ouedraogo, G. Badoum, A. Matteelli
Journal: European Respiratory Journal (2008)

treatment provided for patients with TB, even though the quality of critical care has improved dramatically since the beginning of this study [2].In Korea, directly observed therapy (DOT) is not routinely followed [1].Instead, daily therapy is given, even during the continuation phase, in almost 100% of patients.Ethambutol is usually prescribed for the entire 6 months.These are probably the reasons why TB has been controlled successfully in Korea, despite the fact that DOT has not been adopted.The prevalence of TB dropped dramatically from 5.1% in 1965 to 1.0% in 1995 [3].In our present study [2], because all patients were supported by mechanical ventilation, anti-TB drugs were taken via nasogastric tube or gastrostomy tube, and it was not possible to skip medications.