tbuckley/cabot-search huggingface dataset:
(30) Conjunctival melanoma
Authors: G. Dootson, I. Sarkany
Journal: British Journal of Dermatology (1988)

Journal Article (30) Conjunctival melanoma Get access G. Dootson, G. Dootson Search for other works by this author on: Oxford Academic Google Scholar I. Sarkany I. Sarkany Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 119, Issue s33, 1 July 1988, Page 87, https://doi.org/10.1111/j.1365-2133.1988.tb05433.x Published: 01 July 1988
A Context Study of Psychological Conditions Prior to Shifts in Blood Pressure
Authors: Rolf Adler, Jürg M. Herrmann, Nikolaus Schäfer, Thomas Schmidt, Othmar W. Schonecke, Thure v. Uexküll
Journal: Psychotherapy and Psychosomatics (1976)

Shifts in intra-arterially registered blood pressure during an initial interview were studied in 4 young patients with essential hypertension by means of the Symptom Context Method. The relationships found between both formal variables and thematic categories of speech anteceding blood pressure shifts were in terms of patient and interviewer. All patients showed an increased rate of speech before blood pressure elevations, and in 3 of them the thematic content of 'personal failing, personal failure', 'loss of status of being useful and needed', and 'failure of others to meet the standards of the patient' was more often present before pressure elevations than decreases.
Detection of α-fetoprotein mRNA, an indicator of hematogenous spreading hepatocellular carcinoma, in the circulation: A possible predictor of metastatic hepatocellular carcinoma
Authors: Masayuki Matsumura, Yasuro Niwa, Naoya Kato, Yutaka Komatsu, Shuichiro Shiina, Takao Kawabe, Takeo Kawase, Hiroshi Toyoshima, Masashi Ihori, Yasushi Shiratori, Masao Omata
Journal: Hepatology (1994)

We attempted to detect circulating hepatocellular carcinoma by demonstrating hepatocyte-associated mRNA in the nuclear cell component of peripheral blood using nested reverse transcription—polymerase chain reaction because of the extremely small number of tumor cells in the circulation. Albumin mRNA was demonstrated not only in the liver tissue (hepatocytes) and HepG2 cells but also in nuclear cells of the blood from normal healthy volunteers (neutrophils and lymphocytes) by reverse transcription—polymerase chain reaction. In contrast, α-fetoprotein mRNA was demonstrated in the liver tissue, as well as in HepG2 cells, but not in peripheral blood of normal healthy volunteers, indicating the possibility of using α-fetoprotein mRNA for detection of benign and malignant hepatocytes among the population of neutrophils and lymphocytes. α-Fetoprotein mRNA in peripheral blood was detected in 17 of 33 cases of hepatocellular carcinoma (52%), 2 of 13 cases of cirrhosis (15%) and 2 of 17 cases of chronic hepatitis (12%). α-Fetoprotein mRNA was not demonstrated in 26 cases of normal healthy volunteers (0%). Among the patients with hepatocellular carcinoma, total volume of tumor tissue, maximum size of tumor and serum α-fetoprotein level were markedly increased in the patients with α-fetoprotein mRNA in blood. In addition, α-fetoprotein mRNA was detected in the blood of all 6 patients showing metastasis at extrahepatic organs (100%), in contrast to 11 of 27 cases without metastasis (41%). From these results, we conclude that the presence of α-fetoprotein mRNA in peripheral blood may be an indicator of circulating malignant or benign hepatocytes, which might predict hematogenous spreading metastasis of tumor cells in patients with hepatocellular carcinoma. (Hepatology 1994;20:1418–1425).
Picking Up the Pace — Scale-Up of MDR Tuberculosis Treatment Programs
Authors: Salmaan Keshavjee, Paul E Farmer
Journal: New England Journal of Medicine (2010)

Multidrug-resistant (MDR) tuberculosis is a treatable, airborne infectious disease that killed an estimated 1.5 million people between 2000 and 2009 — an annual rate 10 times that of the H1N1 influenza virus.1,2 During this period, barely 0.5% of the estimated 5 million people who became ill with MDR tuberculosis received treatment with quality-assured second-line drugs. The rest continued to transmit resistant bacteria to others — in their homes, communities, workplaces, and other places where people congregate. The results: an increase, in a number of locales, in the proportion of tuberculosis cases that were MDR; a frightening increase in the . . .
Abstract P3-09-04: Exosome-Specific microRNA Signatures in Combination with Characteristic Surface Markers on the Circulating Exosomes Themselves Provide New Insights into the EMT
Authors: M Paulaitis, N Guzman, K Agarwal, M. Saji
Journal: Cancer Research (2010)

Abstract Background: Recent discoveries have established that cancer tumors exhibit distinct microRNA (miR) expression profiles compared to normal tissues. Detection of miRs in the peripheral blood of cancer patients is possible due to their high stability. This stability is attributed to encapsulation of the miRs inside microvesicles (MVs) where they are protected from endogenous RNase activity in circulation. The tumor cell-secreted MVs of primary interest are circulating exosomes, a subpopulation of MVs distinguished by their relatively small size: 40 to 100 nm in diameter. The isolation of circulating tumor-derived exosomes from the other cell-secreted MVs in peripheral blood is the critical barrier to successful development of a robust assay for cancer-specific miR signatures. We describe the development of such an assay to: (1) capture/isolate circulating exosomes based on characteristic surface markers that correlate with different cell types, and (2) characterize exosome-specific miR signatures based on these surface markers. Materials and Methods: We apply sequential ultracentrifugation to isolate cell-secreted MVs, followed by asymmetric flow field-flow fractionation to selectively separate exosomes based on their intrinsically small size and characteristic surface markers. Isolated exosomes are then screened for surface markers by selectively capturing them on microarrays printed with antibodies against a library of known cell-surface markers for breast cancer. For the assessment of exosome-specific miR signatures, we have devised an antibody microarray assay that also enables the in situ characterization of miR profiles of the captured exosomes locally by qRT-PCR analysis confined to subarrays of printed spots in 50-µl microwells of a custom-designed, multi-well microarray. Assay development has been carried out using two human cancer cell lines (MCF 7 and MDA MB 231) and a non-malignant cell line (MCF 10a) representing pre-malignant cells and cancer of the breast at different epithelial and mesenchymal states. Results: We have used light scattering and cryo-transmission electron microscopy to characterize the size, size distribution, and morphology of exosomes as a sub-population of the secreted MVs from each of the three cell lines. We have also screened for surface markers by selective capturing the exosome sub-populations derived from these cell lines on antibody microarrays, and correlated these surface markers with the miR content of MVs using qRT-PCR analysis. Discussion: Our results demonstrate that correlating the miR signatures of circulating exosomes with characteristic surface markers on these exosomes leads to robust distinctions between the three cell lines that could not be achieved using either analysis or characterization alone. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr P3-09-04.
Pleiotrophin is highly expressed by myeloma cells and promotes myeloma tumor growth
Authors: Haiming Chen, Melinda S. Gordon, Richard A. Campbell, Mingjie Li, Cathy S. Wang, Hee Jin Lee, Eric Sanchez, Steven J. Manyak, Dorina Gui, Dror Shalitin, Jonathan Said, Yunchao Chang, Thomas F. Deuel, Stavroula Baritaki, Benjamin Bonavida, James R. Berenson
Journal: Blood (2007)
Marked Elevation of Brain Natriuretic Peptide Levels in Pericardial Fluid Is Closely Associated With Left Ventricular Dysfunction
Authors: Terumitsu Tanaka, Koji Hasegawa, Masatoshi Fujita, Shun-Ichi Tamaki, Ario Yamazato, Yasuki Kihara, Ryuji Nohara, Shigetake Sasayama
Journal: Journal of the American College of Cardiology (1998)
Measurement of internal left ventricular diameter by a tracking sonomicrometer
Authors: E. B. STINSON, G. RAHMOELLER, P. L. TECKLENBERG
Journal: Cardiovascular Research (1974)

Journal Article Measurement of internal left ventricular diameter by a tracking sonomicrometer Get access EDWARD B. STINSON, EDWARD B. STINSON 1 From the Clinic of Surgery, National Heart and Lung Institute, Bethesda, Maryland, USA 1Present address and address for reprints: Department of Surgery, Cardiovascular Section, Stanford University Medical Centre, Stanford, California 94305, USA. Search for other works by this author on: Oxford Academic PubMed Google Scholar GLENN RAHMOELLER, GLENN RAHMOELLER From the Clinic of Surgery, National Heart and Lung Institute, Bethesda, Maryland, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar PAUL L. TECKLENBERG PAUL L. TECKLENBERG From the Clinic of Surgery, National Heart and Lung Institute, Bethesda, Maryland, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 8, Issue 2, March 1974, Pages 283–289, https://doi.org/10.1093/cvr/8.2.283 Published: 01 March 1974
Impact of the Affordable Care Act on health insurance access, coverage, and cost for refugees resettled in the USA
Authors: P Agrawal, A K Venkatesh
Journal: The Lancet Global Health (2015)

BackgroundRefugees arriving in the USA are a vulnerable population with limited access to health insurance. The Affordable Care Act (ACA) has been heralded as an opportunity to reduce health-care disparities by improving access to health insurance. However there is substantial between-state variation with respect to enactment of Medicaid expansion and implementation of health-care insurance exchanges. We sought to describe the relation between 2012 refugee resettlement patterns and health-care insurance improvements created by the ACA.MethodsWe conducted a cross-sectional analysis of 2012 refugee resettlement data from all 50 states and the District of Columbia. We obtained data from the Office of Refugee Resettlement, the Kaiser Family Foundation, and the US Government health insurance website healthcare.gov. We compared resettlement data for states that have expanded Medicaid and implemented Health Insurance Exchange schemes and those that have not. We used the Wilcoxon rank sum test to investigate whether there was a relation between the price of insurance policies in each state and refugee resettlement rates there.FindingsIn 2012, 58 238 refugees from 85 countries were resettled in the USA. 32 389 refugees were resettled to 27 states that expanded Medicaid coverage under the ACA (median 555, range 0–5173 refugees per state). This rate did not differ from that for the 25 849 refugees who were resettled to 24 states not expanding Medicaid coverage (median 775 [0–5293]; p=0·7341). The median number of refugees resettled to the 17 states creating health insurance exchanges was 817 (1–5173), which was no higher than the median number of refugees resettled to the 34 states using the federal insurance exchange (601 [0–5923]; p=0·4906). The median monthly premium for a bronze health insurance plan did not differ between states with insurance exchanges ($232) and states with only the federal exchange ($264; p=0·1365).InterpretationIn 2012, more refugees were resettled to states that have not expanded Medicaid or created state health insurance exchanges. Because many states have small or no refugee resettlement programmes, the difference in distribution of resettlement based on state insurance policies does not reach statistical significance. Future research should explore the effect of these differences in state policies on refugee health outcomes. Policymakers should consider between-state variation in access to insurance when devising refugee resettlement strategies.FundingNone.
Hepatic Hydatid Cyst causing Suprarenal Haemorrhage
Authors: L. W. Godfrey
Journal: BMJ (1947)

Hydatid disease in the British Isles is comparatively rare, and almost all the cases that have occurred originated in a small endemic area in South Wales.The following case presented a very unusual clinical picture.I have been unable to trace any similar case in the literature, although Barnett (1941) records haemorrhage as a cause of death in six cases of abdo- minal hydatid cyst, and Dew (1928) describes a case of haemorrhage due to anaphylaxis. Case ReportThe patient, a fitter and tester aged 29, was born in Abertillery, Monmouthshire.He was described as being delicate from birth, and at the age of 14 was suspected of having tuberculosis and went to live on a farm at Gilwern, Monmouthshire, where he stayed for three years.He left South Wales at the age of 21 and had since lived in Ipswich.He had never been abroad.In July, 1944, he had an attack of jaundice, with vomiting and abdominal pain, lasting six Weeks.A similar attack occurred in December, 1944, and lasted eight weeks.Following these attacks his general health was impaired, he appeared languid, fainted fre- quently, and had considerable flatulence.On May 27, 1946, he developed upper abdominal pain, diarrhoea, and flatulence, followed next day by jaundice and vomiting, with pale-yell.owstools and dark urine.He was seen by Mr. Langley as an out-patient on May 30, when he was noted to be a well-built man, with moderate jaundice, clean tongue, and good teeth.The liver was enlarged to 4 in.(10 cm.) below the costal margin, with slight tenderness in the gall-bladder region, but the gall-bladder was not palpable.Rectal examination revealed no abnormality.At 8 p.m. on June 1 he was admitted to the Ipswich Borough General Hospital, having become dyspnoeic the previous evening and having passed no urine for twelve hours.On examination he was found to be deeply jaundiced and moderately dyspnoeic, but not distressed or cyanosed.The temperature was 1030 F. (39.4°C.), pulse 132, respirations 36.His pulse was of poor volume and his blood pressure was 80/50.No abnormality was found in the heart or chest on clinical examination.His liver was enlarged, as above, and very tender, with tenderness spreading down to the umbilicus.The spleen was not palpable.Investigations.-The following results were found on May 30.Urine: S.G. 1030, bile +++.Blood count: Hb, 118%; R.B.C., 5,240,000 per c.mm.; reticulocytes, 0.5%; W.B.C., 10,300 per c.mm. (neutrophil polymorphs 52%, lymphocytes 40%, monocytes 8%).Van den Bergh: strongly positive direct reaction.Serum bilirubin, 17.1 mg. per 100 ml.Fragility test normal.W.R. negative.Radiographs of the chest on admission showed congestion of both lung bases and raised diaphragm.
107. Sustained Stable Gene Expression Induced by a Cytoplasmic RNA-Virus Vector Based on Mutant Sendai Virus Strain cl.151
Authors: Ken Nishimura, Hiroaki Segawa, Takahiro Goto, Mariko Morishita, Takemasa Sakaguchi, Tetsuya Yoshida, Kozo Takayama, Mahito Nakanishi
Journal: Molecular Therapy (2005)

Top of pageAbstract
Sendai virus (SeV)|[mdash]|mouse para-influenza virus type 1|[mdash]|is a prototype virus of the Paramyxoviridae with a non-segmented negative strand RNA genome. SeV can infect a wide variety of mammalian cells in vitro and in vivo, and directs high-level gene expression from cytoplasmic viral genome RNA. As it is not pathogenic to humans, it promises to be a unique potential viral vector for human gene therapy while avoiding genotoxicity. However, current SeV vectors induce gene expression only transiently, partly because they are based on the SeV Z strain with lytic replication. To overcome this transient nature of gene expression, we have developed a vector based on the mutant SeV strain, which is capable of persistent infection.

The temperature-sensitive mutant cl.151 strain, originally isolated from an SeV carrier culture (Yoshida et al., Virology 92, 139|[ndash]|154, 1979), has a defect in the late step of viral assembly (Kondo, et al., J. Biol. Chem., 268, 21924|[ndash]|21930, 1993). This mutant form readily establishes persistent infection in various cultured cells at the nonpermissive temperature (38 |[deg]|C), and expresses the viral antigen stably. We cloned the cDNA covering the entire RNA genome of this strain (15,384 nt). It has 50 nucleotide substitutions and 35 amino acid substitutions compared with the genome of the parental Nagoya strain. Using this cDNA as a starting material, we prepared recombinant SeVs carrying marker genes (for green fluorescent protein, GFP, and luciferase, Luc) by standard reverse genetics. These marker genes, flanked by transcription initiation and termination signals, were inserted immediately upstream of the NP (nucleocapsid protein) gene.

The resulting cl.151-based recombinant SeV forms (r151-Luc and r151-GFP) showed temperature-dependent replication and infected various cultured cells persistently. Thus, this cloned cDNA contains all the information required for these characteristics of the cl.151 strain. These recombinant SeVs expressed the marker genes strongly as the Z strain-based vectors, and showed sustained gene expression stably for more than one month. These results strongly suggest the potential utility of this cl.151-based SeV vector as a valuable and non-toxic tool for human gene therapy. Characteristics of marker gene expression induced by cl.151-based SeV vectors are now under examination in animal tissue cells in vivo.
Why are operations cancelled?
Authors: S. Morrissey, T. Alun-Jones, S. Leighton
Journal: BMJ (1989)

and vomiting some mothers were preoccupied and irritable and had to absent themselves.In addition, some resisted overeating by keeping limited amounts of food at home, and this complicated their child's feeding.The mothers' overconcern with shape and weight seemed also to have had an effect.It militated against breast feeding and was associated with undue concern about the children's shape and weight, which in turn affected how they were fed.Furthermore, the mothers' general psychiatric and personality disturbance may have influenced the children's development.'This small case series suggests that further research is needed on the children of mothers with bulimia nervosa.If our findings are confirmed doctors and other health workers should be especially mindful of the feeding and development of such children.We are grateful to the Wellcome Trust for its support and to Helen Woolley, Robert Peveler, and Pavlos Anastasiades for their help.
Morphological and biochemical analyses on fibroblasts and self-produced collagens in a novel three-dimensional culture
Authors: O. ISHIKAWA, A. KONDO, K. OKADA, Y. MIYACHI, M. FURUMURA
Journal: British Journal of Dermatology (1997)

The addition of L-ascorbic acid 2-phosphate (Asc 2-P), which is active and stable under a conventional culture condition, could render dermal fibroblasts to the organization of a dermis-like structure on a plastic dish without any prior treatment. The cell layer was composed of multilayered fibroblasts surrounded by dense extracellular matrices. Confocal microscopic examination disclosed that the fibroblasts in the upper layer were spindle-shaped and those in the lower layer were polygonal. Electron microscopic examination revealed the accumulation of mature collagen fibrils in the intercellular space. These morphological observations suggest that the cell layer may resemble the dermis-like structure. Biochemical analyses revealed that the hydroxyproline content of the cell layer increased in a time-dependent manner, while the monolayer culture system without. Asc 2-P yielded no measurable amount of hydroxyproline. On sodium dodecylsulphate-polyacrylamide gel electrophoresis, neutral insoluble collagens extracted from the cell layer showed the identical electrophoretic pattern to those from the human dermis. In addition, these bands were completely digested by bacterial collagenase. This novel culture system could provide a simple tool with which to investigate the collagen metabolism by fibroblasts under more physiological conditions.
Diagnosing cancer in general practice: when is cancer suspected?
Authors: M Nylenna
Journal: BMJ (1986)

Of 10,278 encounters with patients recorded by 33 general practitioners in a defined Norwegian population during a two month period, 429 (4.2%) were classified as resulting in a follow up for suspected cancer. The patient's fear of cancer was the most important indicator of suspicion of cancer among the doctors. Patients who consulted for digestive and gynaecological problems were most frequently suspected of having cancer. Comparing the pattern of suspicion of cancer with the incidence of cancer in the area showed a relative oversuspicion in young patients and women.
Mutational pattern of hepatitis B virus on sequential therapy with famciclovir and lamivudine in patients with hepatitis B virus reinfection occurring under hbig immunoglobulin after liver transplantation
Authors: Hans L. Tillmann, Christian Trautwein, Thomas Bock, Klaus H. Böker, Elmar Jäckel, Medea Glowienka, Karl Oldhafer, Insa Bruns, Josee Gauthier, Lynn D. Condreay, Hans-Rudolf Raab, Michael P. Manns
Journal: Hepatology (1999)

Famciclovir (FCV) and lamivudine (LAM) reduce viral replication in patients with recurrent hepatitis B virus (HBV) infection after orthotopic liver transplantation (OLT). Eighteen of 20 patients with insufficient response to FCV were treated with 100 mg LAM daily after OLT. These patients had shown nonresponse (n = 5), partial response (n = 7), or breakthrough (n = 6) during FCV therapy. Despite passive immunoprophylaxis with hepatitis B immunoglobulin after liver transplantation, HBV reinfection had occurred in 14 of 15 transplanted patients. HBV-DNA levels and the regions A to E of the HBV-DNA polymerase gene were analyzed before and after treatment failure to either therapy. Within 4 weeks on LAM, all but 1 patient showed a 95% average reduction of the HBV-DNA level. As with FCV, we did not observe any severe side-effects attributable to LAM. However, 7 patients developed a breakthrough within 12, 29 (n = 2), 32, 37, 54, and 145 weeks under treatment with LAM associated with the methionine-to-valine signature mutation (M552V) in the YMDD motif in all. With FCV, no unique, but a dominant, resistance pattern with the L528M mutation was identified for patients with breakthrough under FCV. In contrast, nonresponders or patients with partial response to FCV did not exhibit such mutations. Our results indicate that the L528M mutation is a risk factor for LAM breakthrough, because breakthrough during LAM occurred earlier in patients with this mutation (50 ± 10 weeks vs. 120 ± 21 weeks). Because breakthrough on either treatment is frequent for this specific group of patients, the use of combination therapy should be explored.