Negative Rapid HIV Antibody Testing during Early HIV Infection
Authors: Joanne Stekler, Robert W. Wood, Paul D. Swenson, Matthew Golden
Journal: Annals of Internal Medicine (2007)

Letters17 July 2007Negative Rapid HIV Antibody Testing during Early HIV InfectionJoanne Stekler, MD MPH, Robert W. Wood, MD, Paul D. Swenson, PhD, and Matthew Golden, MD, MPHJoanne Stekler, MD MPHFrom Public Health - Seattle and King County, and University of Washington, Seattle, WA 98195., Robert W. Wood, MDFrom Public Health - Seattle and King County, and University of Washington, Seattle, WA 98195., Paul D. Swenson, PhDFrom Public Health - Seattle and King County, and University of Washington, Seattle, WA 98195., and Matthew Golden, MD, MPHFrom Public Health - Seattle and King County, and University of Washington, Seattle, WA 98195.Author, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-147-2-200707170-00022 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Background: Rapid HIV tests are generally considered to be as sensitive as second-generation enzyme immunoassays (EIAs) in detecting recent HIV antibody seroconversion.Objective: To describe 3 individuals who had early HIV infection that was not identified by rapid HIV antibody testing but would have been detected by an EIA.Case Report: The first patient had last tested negative for HIV 2 months earlier. He sought HIV testing at the Public Health–Seattle & King County (PHSKC) Sexually Transmitted Disease Clinic, Seattle, Washington, 1 week after having unprotected anal intercourse with an HIV-positive male partner. His fingerstick blood specimen was HIV-negative by ...References1. Stekler J, Swenson PD, Wood RW, Handsfield HH, Golden MR. Targeted screening for primary HIV infection through pooled HIV-RNA testing in men who have sex with men. AIDS. 2005;19:1323-5. [PMID: 16052089] CrossrefMedlineGoogle Scholar2. Kuun E, Brashaw M, Heyns AD. Sensitivity and specificity of standard and rapid HIV-antibody tests evaluated by seroconversion and non-seroconversion low-titre panels. Vox Sang. 1997;72:11-5. [PMID: 9031494] CrossrefMedlineGoogle Scholar3. Samdal HH, Gutigard BG, Labay D, Wiik SI, Skaug K, Skar AG. Comparison of the sensitivity of four rapid assays for the detection of antibodies to HIV-1/HIV-2 during seroconversion. Clin Diagn Virol. 1996;7:55-61. [PMID: 9077430] CrossrefMedlineGoogle Scholar4. Beelaert G, Vercauteren G, Fransen K, Mangelschots M, De Rooy M, Garcia-Ribas S, et al. Comparative evaluation of eight commercial enzyme linked immunosorbent assays and 14 simple assays for detection of antibodies to HIV. J Virol Methods. 2002;105:197-206. [PMID: 12270653] CrossrefMedlineGoogle Scholar5. Wawer MJ, Gray RH, Sewankambo NK, Serwadda D, Li X, Laeyendecker O, et al. Rates of HIV-1 transmission per coital act, by stage of HIV-1 infection, in Rakai, Uganda. J Infect Dis. 2005;191:1403-9. [PMID: 15809897] CrossrefMedlineGoogle Scholar Author, Article, and Disclosure InformationAuthors: Joanne Stekler, MD MPH; Robert W. Wood, MD; Paul D. Swenson, PhD; Matthew Golden, MD, MPHAffiliations: From Public Health - Seattle and King County, and University of Washington, Seattle, WA 98195.Note added in proof: In the 5 months between acceptance and publication of this letter, 3 additional individuals have been identified who tested HIV-negative by OraQuick but HIV-positive by second-generation EIA.Acknowledgments: The authors thank the University of Washington Primary Infection Clinic and the second individual for consenting to share follow-up HIV testing results. They also thank the AIEDRP AIN503/ACTG A5217 protocol team for allowing us to present results of the detuned EIA.Grant Support: Dr. Stekler was funded by a National Institutes of Health grant (K23 AI65243).Disclosures: None disclosed. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoRepeatedly False-Negative Rapid HIV Test Results in a Patient with Undiagnosed Advanced AIDS Patricia Brown , Joseph R. 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Merline, PhD, Diane Levine, MD, and Lucio Román Minces, MDRepeat Confirmatory Testing for Persons with Discordant Whole Blood and Oral Fluid Rapid HIV Test Results: Findings from Post Marketing Surveillance 17 July 2007Volume 147, Issue 2Page: 147-148KeywordsHematologic testsHIVHIV infectionsHIV-1PatientsPrevention, policy, and public healthRibosomal DNARNASexually transmitted diseasesWestern blot ePublished: 17 July 2007 Issue Published: 17 July 2007 Copyright & PermissionsCopyright © 2007 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...