tbuckley/cabot-search huggingface dataset:
Clonal expansion and somatic hypermutation of V(H) genes of B cells from cerebrospinal fluid in multiple sclerosis.
Authors: Y Qin, P Duquette, Y Zhang, P Talbot, R Poole, J Antel
Journal: Journal of Clinical Investigation (1998)

The cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients is characterized by increased concentrations of immunoglobulin (Ig), which on electrophoretic analysis shows restricted heterogeneity (oligoclonal bands). CSF Ig is composed of both serum and intrathecally produced components. To examine the properties of intrathecal antibody-producing B cells, we analyzed Ig heavy-chain variable (V(H)) region genes of B cells recovered from the CSF of 12 MS patients and 15 patients with other neurological diseases (OND). Using a PCR technique, we could detect rearrangements of Ig V(H) genes in all samples. Sequence analysis of complementarity-determining region 3 (CDR3) of rearranged VDJ genes revealed expansion of a dominant clone or clones in 10 of the 12 MS patients. B cell clonal expansion was identified in 3 of 15 OND. The nucleotide sequences of V(H) genes from clonally expanded CSF B cells in MS patients demonstrated the preferential usage of the V(H) IV family. There were numerous somatic mutations, mainly in the CDRs, with a high replacement-to-silent ratio; the mutations were distributed in a way suggesting that these B cells had been positively selected through their antigen receptor. Our results demonstrate that in MS CSF, there is a high frequency of clonally expanded B cells that have properties of postgerminal center memory or antibody-forming lymphocytes.
Sex Education in Schools— A Polemic
Authors: Florence M. Lewis
Journal: JAMA (1969)

<h3>To the Editor:—</h3> The American Medical Association does a disservice to itself and the American people by attempting to label opponents of sex education programs as political extremists or uninformed. You have neglected to mention a third category—namely, well-informed psychiatrists and at least one well-known child psychoanalyst. As an AMA and APA member, who served for a year on a medical society's Family Life Education (FLE) committee, and as a mother of four children in the public school system, I cannot plead ignorance, nor am I now, nor have I ever been a member of the John Birch Society. I am an active opponent of my county's FLE program, and, I believe, for sound psychiatric reasons. I weary of the effort to shut off debate by the use of labels. The AMA may or may not be a tool of the Communist party. It is, however, in my opinion, lacking
THE EFFECT OF ATROPINE UPON THE OUTPUT OF THE HEARTS OF NORMAL MEN
Authors: W. Carter Smith, C. Sidney Burwell, Michael J. DeVito
Journal: Journal of Clinical Investigation (1928)

20 a.m.76 0.74 -21 144 3.27 4,400 58 May 6, 11:50 a.m.66 0.77 -15 158 3.45 4,580 69 * 1 to 2 minutes before this time the subject received an intravenous injection of 1.2 mgm.atropine sulphate.Immediately thereafter the determination of the cardiac output was begun.
Association between female sex, IL28B genotype, but also DQB1*0301 allele and the outcome of acute hepatitis C virus infection
Authors: Laurent Alric, Delphine Bonnet, Marylise Fort
Journal: Hepatology (2014)

We thank Grebely et al.1 for their recent InC study confirming that the IL28B genotype and female sex are associated with the spontaneous clearance of hepatitis C virus (HCV). However, we and others showed that spontaneous HCV resolution is associated with another genetic factor more than 10 years ago. This factor is the DQB1*0301 allele of the major histocompatibility complex class II.2 The IL28B genotype was shown to be the most important factor associated with a sustained virologic response to pegylated-interferon ribavirin therapy3 and also with the spontaneous outcome of HCV infection.4 However, the study4 showing that genetic variations in IL28B is associated with spontaneous clearance of HCV focused on a single nucleotide polymorphism and was not a genome-wide association study (GWAS). A new multicenter collaborative study5 on 919 North American and European subjects whose HCV was resolved spontaneously was conducted to explain the conflicting results.2, 4 It was shown5 that IL28B rs12979860 and DQB1*0301 rs4272729 alleles were independently associated with the spontaneous resolution of HCV. Surprisingly, these results are not discussed by Grebely et al.1 The InC study1 is important because the authors confirmed prospectively the well-known IL28B polymorphism and reported that being a female is also associated with the clearance of an acute HCV infection. Our previous cohort studies2, 6 also found this statistically significant association with female sex (P < 0.004), but it was not an independent factor in multivariate analysis. We also reported that viral genotype plays no role in spontaneous resolution of HCV infection.2, 6 We are disappointed that the InC study did not test the DQB1*0301 allele in each subject. It is technically easy to do so and not costly. We believe that they would have found that this host factor is also associated with spontaneous HCV resolution. We suggest that female sex will not be an independent predictor of spontaneous HCV clearance when both these genetic factors are included in a multivariate analysis. Laurent Alric, Ph.D., M.D.1 Delphine Bonnet, M.D.1 Marylise Fort, M.D.2 1Internal Medicine Digestive Department Purpan Hospital UMR152, IRD Toulouse III University, Toulouse, France 2Immunology Unit Rangueil Hospital, Toulouse, France
670 Morbidly obese patients with or whitout nash and type 2 diabetes have increased inflammatory C-reactive protein in both liver and adipose tissue
Authors: R. Anty, S. Bekri, N. Luciani, M.C. Saint Paul, M. Dahman, A. Iannelli, I. Ben Amor, A. Staccini-Myx, P.M. Huet, J. Gugenheim, J.L. Sadoul, Y. Le Marchand-Brustel, A. Tran, P. Guall
Journal: Journal of Hepatology (2006)
THE GRAVES SYNDROME
Authors: Israel Bram
Journal: JAMA (1931)

To the Editor:  —Your editorial on the Graves syndrome in the issue of Dec. 27, 1930, and its criticism by Percy Fridenberg, January 31, page 376, merit further comment. Fridenberg asserts that the psychic factor  certainly not of prime importance and probably not essential to the development of the  The emotional symptoms are possibly a result, not a cause, of the disease.  The average clinician must heartily agree with the implications in your editorial and with the views of Eli Moschcowitz (The Nature of Graves' Disease, Arch. Int. Med . 46 :610 [Oct.] 1930), A. S. Warthin (The Constitutional Entity of Exophthalmic Goiter and So-Called Toxic Adenoma, Ann. Int. Med . 2 :553 [Dec.] 1928) and others in the opinion that exophthalmic goiter, whatever its ultimate cause, is a constitutional disorder in which the psyche is of major etiologic importance. Life is too limited to comprehend the ultimate cause of
Derivation of functional mature neutrophils from human embryonic stem cells
Authors: Yasuhisa Yokoyama, Takahiro Suzuki, Mamiko Sakata-Yanagimoto, Keiki Kumano, Katsumi Higashi, Tsuyoshi Takato, Mineo Kurokawa, Seishi Ogawa, Shigeru Chiba
Journal: Blood (2009)
The addition of sirolimus to tacrolimus/methotrexate GVHD prophylaxis in children with ALL: a phase 3 Children’s Oncology Group/Pediatric Blood and Marrow Transplant Consortium trial
Authors: Michael A. Pulsipher, Bryan Langholz, Donna A. Wall, Kirk R. Schultz, Nancy Bunin, William L. Carroll, Elizabeth Raetz, Sharon Gardner, Julie M. Gastier-Foster, Denise Howrie, Rakesh K. Goyal, James G. Douglas, Michael Borowitz, Yvonne Barnes, David T. Teachey, Candace Taylor, Stephan A. Grupp
Journal: Blood (2014)
Gene Expression Changes Related to Endocrine Function and Decline in Reproduction in Fathead Minnow ( <i>Pimephales promelas</i> ) after Dietary Methylmercury Exposure
Authors: Rebecca Klaper, Christopher B. Rees, Paul Drevnick, Daniel Weber, Mark Sandheinrich, Michael J. Carvan
Journal: Environmental Health Perspectives (2006)

Methylmercury (MeHg) is a known neurotoxic agent, but the mechanisms by which MeHg may act on reproductive pathways are relatively unknown. Several studies have indicated potential changes in hormone levels as well as declines in vertebrates with increasing dietary MeHg exposure.The purpose of this study was to identify alterations in gene expression associated with MeHg exposure, specifically those associated with previously observed changes in reproduction and reproductive biomarkers. Fathead minnows, Pimephales promelas, were fed one of three diets that were similar to documented concentrations of MeHg in the diets of wild invertivorous and piscivorous fish. We used a commercial macroarray in conjunction with quantitative polymerase chain reaction to examine gene expression in fish in relation to exposure to these environmentally relevant doses of MeHg.Expression of genes commonly associated with endocrine disruption was altered with Hg exposure. Specifically, we observed a marked up-regulation in vitellogenin mRNA in individual Hg-exposed males and a significant decline in vitellogenin gene expression in female fish with increasing Hg concentrations. Other genes identified by the macroarray experiment included those associated with egg fertilization and development, sugar metabolism, apoptosis, and electron transport. We also observed differences in expression patterns between male and female fish not related to genes specifically associated with reproduction, indicating a potential physiological difference in the reaction of males and females to MeHg.Gene expression data may provide insight into the mechanisms by which MeHg affects reproduction in fish and indicate how MeHg differs in its effect from other heavy metals and endocrine-disrupting compounds.
Abstract LB-110: Establishment of a chordoma xenograft and in vivo testing of compounds identified by high-throughput screening
Authors: Matteo Trucco, Breelyn A. Wilky, Ola Awad, Preeti Shah, Naheed Gul, Menghang Xia, Ruili Huang, David M. Loeb
Journal: Cancer Research (2012)

Abstract Chordoma is a rare primary bone malignancy that arises in the skull base, spine and sacrum and originates from remnants of the notochord. Therapy primarily consists of surgical resection and radiation. These tumors are typically resistant to conventional chemotherapy, and to date there are no FDA-approved agents for chordoma. The lack of in vivo models of chordoma has impeded the development of new therapies for this tumor. We have established a chordoma xenograft growing in NOD/SCID/IL-2R gamma-null mice. The histology of the xenograft is consistent with a dedifferentiated chordoma. The xenograft expresses the chordoma marker T brachyury, which is aberrantly localized in the cytoplasm. SNP array shows loss of heterozygosity at the p16 locus, and gene expression profiling shows high expression of numerous cancer-related genes, such as ROS1 and CPA4 The NIH Chemical Genomics Center performed high-throughput screening of 2,816 compounds. Two established chordoma cell lines, U-CH1 and UCH2B, were treated and cell viability measured by CellTiter-Glo assay. Cells were incubated for 48 hours with drug concentrations ranging from 0.5nM to 46uM. The screen yielded several compounds that showed activity and two were tested in the xenograft. Compared with mice treated with vehicle alone, both drugs showed a dramatic inhibition of tumor growth. In conclusion, we have established a xenograft model of dedifferentiated chordoma. High-throughput screening of compounds identified compounds that show activity against chordoma. In vivo testing of two identified compounds showed a dramatic reduction of tumor growth. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-110. doi:1538-7445.AM2012-LB-110
Skin grafts as pharmacological agents: pre-wounding of the donor site
Authors: R.S. KIRSNER, V. FALANGA, F.A. KERDEL, M.H. KATZ, W.H. EAGLSTEIN
Journal: British Journal of Dermatology (1996)

Journal Article Skin grafts as pharmacological agents: pre‐wounding of the donor site Get access R.S. KIRSNER, R.S. KIRSNER University of Miami School of Medicine, Department of Dermatology and cutaneous surgery, Miami, Florida, U.S.A. Robert S.Kirsner, University of Miami Department of Dermatology, PO Box 016250 (R250), Miami, Florida 33136, U.S.A. Search for other works by this author on: Oxford Academic Google Scholar V. FALANGA, V. FALANGA University of Miami School of Medicine, Department of Dermatology and cutaneous surgery, Miami, Florida, U.S.A. Search for other works by this author on: Oxford Academic Google Scholar F.A. KERDEL, F.A. KERDEL University of Miami School of Medicine, Department of Dermatology and cutaneous surgery, Miami, Florida, U.S.A. Search for other works by this author on: Oxford Academic Google Scholar M.H. KATZ, M.H. KATZ University of Miami School of Medicine, Department of Dermatology and cutaneous surgery, Miami, Florida, U.S.A. Search for other works by this author on: Oxford Academic Google Scholar W.H. EAGLSTEIN W.H. EAGLSTEIN University of Miami School of Medicine, Department of Dermatology and cutaneous surgery, Miami, Florida, U.S.A. Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 135, Issue 2, 1 August 1996, Pages 292–296, https://doi.org/10.1111/j.1365-2133.1996.tb01164.x Published: 01 August 1996 Article history Accepted: 24 October 1995 Published: 01 August 1996
Abstract 116: Methylcytosine binding domain protein 2(MBD2) dependent proliferation and survival of breast cancer cells
Authors: Omar Y. Mian, Shou Z. Wang, Sheng Z. Zhu, Harry D. Bear, Gordon D. Ginder
Journal: Cancer Research (2011)

Abstract Methyl Cytosine Binding Domain Protein 2 (MBD2) functions as an interpreter of DNA methylation by binding promoter sequences with high methylated CpG content and recruiting a Nuclear Remodeling and Deacetylase (NuRD) co-repressor complex. MBD2 has been shown to bind to and mediate repression of methylated tumor suppressor genes in cancer cells where re-patterning of CpG methylation and associated gene silencing is common. We have investigated the role of MBD2 in breast cancer cell growth and tumor suppressor gene expression. We show that stable shRNA mediated knockdown of MBD2 leads to significant growth suppression of cultured human mammary epithelial cancer lines, SK-BR-3 (92%); MDA-MB-231 (77%); MDA-MB-435 (94%); with the peak anti-proliferative effect occurring only after sustained, stable knockdown and lasting over 4 weeks. MBD2 knockdown also leads to markedly decreased propensity for these aggressive breast cancer cell lines to form in vivo xenograft tumors in BALB/C nu/nu mice. The growth effects of MBD2 knockdown are accompanied by de-repression of specific tumor suppressor genes including DAPK1 and KLK10. Chromatin immunoprecipitation assays and bisulfite sequencing demonstrate MBD2 binding directly to the hyper-methylated CpG-rich promoters of both DAPK1 and KLK10. Expression of an shRNA-resistant MBD2 protein results in restoration of growth and re-silencing of the MBD2 dependent tumor suppressor genes. Our data suggest that uncoupling CpG-methylation from repressive chromatin remodeling and histone modifications by removing MBD2 is sufficient to initiate and maintain tumor suppressor gene transcription and suppress neoplastic cell growth. These results demonstrate a role for MBD2 in aggressive breast cancer cell growth and provide support for the prospect of targeting MBD2 therapeutically. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 116. doi:10.1158/1538-7445.AM2011-116
Case 2-1971
Authors: Richard C. Cabot, Benjamin Castleman, Betty U. McNeely, George P. Baker, John B. Blennerhassett
Journal: New England Journal of Medicine (1971)

Presentation of CaseA 27-year-old woman was admitted to the hospital because of weakness.She had been well until five and a half years previously, when bilateral cervical lymphadenopathy developed. Four months later fever and fatigue occurred, and a biopsy of a cervical lymph node disclosed Hodgkin's lymphoma, nodular-sclerosis type; a bone-marrow biopsy was negative. After a course of radiotherapy in a dose of 3500 rads delivered to the mediastinum and 1600 rads to each supraclavicular region no lymph nodes were palpable, and the patient was asymptomatic. Four and a half years before admission an appendectomy was performed; no evidence . . .
Bronchial and Peripheral Murine Lung Carcinomas Induced by T790M-L858R Mutant EGFR Respond to HKI-272 and Rapamycin Combination Therapy
Authors: Danan Li, Takeshi Shimamura, Hongbin Ji, Liang Chen, Henry J. Haringsma, Kate McNamara, Mei-Chih Liang, Samanthi A. Perera, Sara Zaghlul, Christa L. Borgman, Shigeto Kubo, Masaya Takahashi, Yanping Sun, Lucian R. Chirieac, Robert F. Padera, Neal I. Lindeman, Pasi A. Jänne, Roman K. Thomas, Matthew L. Meyerson, Michael J. Eck, Jeffrey A. Engelman, Geoffrey I. Shapiro, Kwok-Kin Wong
Journal: Cancer Cell (2007)
Association between chronic hepatitis C infection and hepatocellular carcinoma in a Scottish population.
Authors: G H Haydon, L M Jarvis, P Simmonds, D J Harrison, O J Garden, P C Hayes
Journal: Gut (1997)

BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common cancers in the world. The geographical prevalence varies considerably in different countries and Scotland is regarded as an area of low risk for the disease. AIMS: To assess the association between chronic hepatitis C infection (HCV) and HCC in a population of patients presenting to a single hospital. PATIENTS: One hundred and fourteen cases of histologically confirmed liver cancer presenting to the Royal Infirmary of Edinburgh between 1985 and 1994 were examined. METHODS: Of 114 cases of HCC, 80 samples of stored sera were available. Samples positive for HCV Ab were genotyped by restriction fragment length polymorphism analysis of HCV c-DNA. A population of 29 cirrhotic patients (diagnosed between 1985 and 1994) with chronic HCV infection was also genotyped. RESULTS: Chronic HCV infection was a major risk factor (30% of tested HCC patients) identified. HCV genotype 1b was predominant (16 of 20 patients). The time from HCV transmission to development of cancer ranged from 10 to 50 years (median 30). In the cirrhotic patient population, a broader distribution of genotypes was present (genotype 1a: 7; genotype 1b: 8; genotype 2b: 3; genotype 3a: 8 and genotype 4: 2). However, this population was significantly younger. (Mean (SD) 52 (14.5) years) (p = 0.0002) and demonstrated a significantly shorter duration of infection: range 10-40 years (median: 19). CONCLUSION: There is a strong association between chronic HCV infection, cirrhosis, and hepatocarcinogenesis in this Scottish population. The study was unable to distinguish whether the high prevalence of genotype 1b in the HCC population reflected increased oncogenicity in itself, or whether 1b was simply the most prevalent genotype in Scotland when these patients were infected.
THEORETICAL MODEL FOR THE RELATIONSHIP BETWEEN THE NUMBER OF TACKLES A PLAYER ENGAGES IN, TACKLE INJURY RISK AND PERFORMANCE IN RUGBY UNION
Authors: S Hendricks, L Micheal
Journal: British Journal of Sports Medicine (2014)

Background The tackle in rugby union is a physical contest between opposing players contending for territory and ball possession. During an 80-minute rugby match, a player will physically engage in the tackle contest between 10 to 35 times. For a player to repeatedly engage safely and effectively in the tackle, a high level of skill and physical tolerance is required. Objective Understanding the physical demands of a tackle is necessary for the design and development of proper training drills, equipment, planning and management of training, recovery between training sessions and matches, minimising the risk of injury., and to replicate the event in the laboratory for research. Design Theoretical model. Setting Worldwide rugby playing population. Participants All levels of play. Risk factor assessment Magnitude of impact (energy load), number of tackles (during a match or over a season), muscle damage (micro trauma). Main outcome measurements Tackle injury risk and tackle performance. Results It is theorised that players have a capacity to endure repeated high energy impact tackle situations. However, there is an upper limit, beyond which, the risk of injury is substantially increased, and tackle performance is noticeably decreased. This upper limit is reached either through one or two very high-energy impact contact situations or, accumulates over a match or season. Effective tackle skill training, proper physical conditioning, strength, power, equipment and attitude/motivation can offset the upper limit. Conclusions Building on previous work in conjunction with the findings of Hendricks et al, a theoretical model for the relationship between the number of tackles a player engages in (acute or chronic fatigue), magnitude of impact (energy load), markers of muscle damage (micro trauma) and how this relationship interacts with tackle injury risk (tolerance overload or reduction) and tackle performance is proposed.
The Iraqi Refugee Crisis
Authors: Hani Mowafi, Paul Spiegel
Journal: JAMA (2008)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
The Role of Compulsiveness in the Normal Physician
Authors: Glen O. Gabbard
Journal: JAMA (1985)

This article presents some observations from a workshop setting about the role of compulsiveness in the normal physician. Case examples illustrate the effect of this character trait on the professional, personal, and family life of the typical physician. Doubt, guilt feelings, and an exaggerated sense of responsibility form a compulsive triad in the personality of the physician. This triad manifests itself in both adaptive and maladaptive ways. This article focuses primarily on the maladaptive, including difficulty in relaxing, reluctance to take vacations from work, problems in allocating time to family, an inappropriate and excessive sense of responsibility for things beyond one's control, chronic feelings of "not doing enough," difficulty setting limits, hypertrophied guilt feelings that interfere with the healthy pursuit of pleasure, and the confusion of selfishness with healthy self-interest.
HMG-CoA reductase inhibition by atorvastatin reduces neointimal inflammation in a rabbit model of atherosclerosis
Authors: Carmen Bustos, Miguel A Hernández-Presa, Mónica Ortego, José Tuñón, Luis Ortega, Fernando Pérez, Cristina Díaz, Gonzalo Hernández, Jesús Egido
Journal: Journal of the American College of Cardiology (1998)