tbuckley/cabot-search huggingface dataset:
Partial repression of human γ-globin genes by LCR element HS3 when linked to β-globin genes and LCR element HS2 in MEL cells
Authors: Christian J. Stoeckert, Hui Cheng
Journal: American Journal of Hematology (1996)

Clues for overcoming fetal (γ-) globin gene repression in adult human erythroid cells may come from understanding why repression of isolated γ-globin genes has not previously been achieved in the adult erythroid environment of mouse erythroleukemia cells (MEL). Repression of human γ-globin genes has been demonstrated in MEL cells when transferred as part of the entire β-globin gene cluster packaged in chromatin. Major differences in these approaches are prior packaging into chromatin and the presence of additional sequences, notably from the locus control region (LCR). In this report we focus on the contribution to γ-globin gene repression that multiple elements of the LCR may have. We first show preferential activation of β-globin genes over γ-globin genes in MEL cells when linked to each other and to LCR sequences containing the core elements of DNase I hypersensitive sites 4, 3, and 2. Removal of the HS4 element had no effect, however, removal of the 225 bp HS3 core element resulted in a five-fold increase in γ-globin gene expression. The enhancer 3′ to the Aγ-globin gene also had no apparent effect on γ-globin gene expression. These results provide first evidence of γ-globin gene repression involving the core region of HS3 in the presence of the core region of HS2 and a β-globin gene. A mechanism for repression involving sequestration of the γ-promoter away from the strong enhancer activity of HS2 is proposed. © 1996 Wiley-Liss, Inc.
Abstract P4-05-07: Molecular profiles of screen detected breast cancers: Final results of Turkish Bahcesehir breast cancer screening project
Authors: N Cabioglu, S Ozkan-Gurdal, A Kayhan, N Ozaydin, B Ozcinar, E Aribal, V Ozmen
Journal: Cancer Research (2013)

Abstract Objectives: Turkish Bahcesehir Breast Cancer Screening Project is a 10-year organized population based screening program carried out in one of the largest counties of Istanbul, Turkey. The aim of our study is to examine the biological features of screen detected breast cancers detected during the initial 4-year study period as an interim analysis. Study Design and Methods: Between January 2009 and May 2013, a total of 6298 women with ages 40–69 years were recruited in this prospective study. Two-view mammographies were obtained by 2-year intervals, and classified according to Breast Imaging Reporting and Data System of the American College of Radiology (ACR). Clinicopathological and biological tumor characteristics were analysed for those diagnosed with breast cancer. Tumors were stained for estrogen (ER) and progesterone receptors (PR), HER2-neu and Ki-67 by immunohistochemistry. Results: A total of 57 breast cancers (%0.9) were detected during the study period. The median age was 50 (40-70). The majority of patients (72%) were stage 0 or 1, whereas 28 patients (49%) were &amp;lt;50 age. Of 57 tumors, 45 (79%) were invasive cancers and 12 (21%) were ductal carcinoma in situ. Forty-eight patients (86%) underwent breast conservation, whereas 37 patients (65%) had sentinel lymph node biopsy only as axillary procedure. Of 45 invasive cancers, 31 (69%) were ductal carcinoma and 8 (18%) were pure lobular cacinoma. Among 45 invasive cancers stained for ER, PR, HER2-neu and Ki67, the majority of them (91%) were ER or PR receptor positive, whereas 11% were HER2-neu positive and 58% had low Ki67 levels (≤15). As molecular subtypes, the majority of them were found to be either luminal A (57%) or luminal B type (34%), whereas other nonluminal HER2 (4%) and triple negative cancers (5%) were less frequently detected. Conclusions: Our findings suggest that the majority of screen-detected breast cancers exhibit either luminal A or B subtype. However, more aggressive subtypes such as nonluminal HER2-neu or triple negative cancer are less likely to be detected by mammographic screening programs, requiring other preventive strategies. Citation Information: Cancer Res 2013;73(24 Suppl): Abstract nr P4-05-07.
[32] MODERATE EXPANSION OF MILAN CRITERIA FOR TRANSPLANTATION FOR HCC DOES NOT IMPACT SURVIVAL NOR RECURRENCE RATES
Authors: H.M. Badran, C. Meyer, R. Adam, A. Plessier, F. Durand, O. Boillot, J. Dumortier, J. Dharancy, H.M. Hilleret, T. Decaens, D. Cherqui, C. Duvoux
Journal: Journal of Hepatology (2007)
Impaired TH2 response in patients with Vav1-deficient common variable immunodeficiency with T-cell defects
Authors: Nagaja Capitani, Amedeo Amedei, Silvia Rossi Paccani, Andrea Matucci, Alessandra Vultaggio, Gianfranco Del Prete, Cosima T. Baldari, Mario M. D'Elios
Journal: Journal of Allergy and Clinical Immunology (2010)
Barrier function parameters in various keratinization disorders: transepidermal water loss and vascular response to hexyl nicotinate
Authors: A.P.M. LAVRIJSEN, E. OESTMANN, J. HERMANS, H.E. BODDE, B.J. VERMEER, M. PONEC
Journal: British Journal of Dermatology (1993)

Journal Article Barrier function parameters in various keratinization disorders: transepidermal water loss and vascular response to hexyl nicotinate Get access A.P.M. LAVRIJSEN, A.P.M. LAVRIJSEN Department of Dermatology, University Hospital Leiden, The Netherlands Dr A.P.M. Lavrijsen. Department of Dermatology. University Hospital, Rijnsburgerweg 10, 2333 AA Leiden. The Netherlands. Search for other works by this author on: Oxford Academic Google Scholar E. OESTMANN, E. OESTMANN Department of Dermatology, University Hospital Leiden, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar J. HERMANS, J. HERMANS Department of Medical Statistics, University of Leiden, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar H.E. BODDE, H.E. BODDE Centre of Bio‐Pharmaceutical Sciences, University of Leiden, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar B.J. VERMEER, B.J. VERMEER Department of Dermatology, University Hospital Leiden, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar M. PONEC M. PONEC Department of Dermatology, University Hospital Leiden, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 129, Issue 5, 1 November 1993, Pages 547–554, https://doi.org/10.1111/j.1365-2133.1993.tb00482.x Published: 01 November 1993 Article history Accepted: 28 April 1993 Published: 01 November 1993
Abstract 3086: The genetic landscape of the childhood liver cancer hepatoblastoma
Authors: Roland Kappler, Melanie Eichenmüller, Franziska Trippel, Tim M. Strom, Dietrich von Schweinitz
Journal: Cancer Research (2014)

Abstract Hepatoblastoma (HB) is the most common malignant liver tumor in children with a histology that resembles various stages of the developing liver. To explore the genetic origin of this cancer, we performed whole-exome sequencing of 8 tumors and matched germline tissue. In average, we detected only three sequence variants per tumor, which is the lowest mutation rate ever reported for any cancer. Mutations of ß-catenin (CTNNB1) displayed the only recurrent event occurring in all tumors, thereby underscoring the significance of activated Wnt signaling in the genesis of HB. Gene ontology analysis revealed a predominance of candidate genes involved in the regulation of gene expression and transcription, including the nuclear factor (erythroid-derived 2)-like 2 (NFE2L2). Targeted resequencing of a cohort of 50 HB cases and cell lines revealed NFE2L2 mutations in 10% of all tumors. Interestingly, all mutations were located in or adjacent to the DLG and ETGE motifs of the NFE2L2 protein that are known to be recognized from the KEAP1/CUL3 complex for proteasomal degradation. In line with this, cells transfected with mutant NFE2L2 were insensitive to KEAP1-mediated downregulation of NFE2L2 signaling. Clinically, overexpression of the NFE2L2 target gene NQO1 in tumor tissues was significantly associated with high tumor stage, metastasis, and poor outcome. These data suggest that activation of NFE2L2-KEAP1 signaling through mutations in NFE2L2 is a prominent characteristic in pediatric liver tumors, thereby advocating inhibition of aberrant NFE2L2 activity as a therapeutic option, especially in high-risk patients with advanced disease. Citation Format: Roland Kappler, Melanie Eichenmüller, Franziska Trippel, Tim M. Strom, Dietrich von Schweinitz. The genetic landscape of the childhood liver cancer hepatoblastoma. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3086. doi:10.1158/1538-7445.AM2014-3086
Abstract 4495: Sym013, novel pan-HER monoclonal antibody mixture, augments radiation response in human lung and head and neck tumors
Authors: David Francis, Shyhmin Huang, Lauryn Werner, Johan Lantto, Ivan D. Horak, Michael Kragh, Paul M. Harari
Journal: Cancer Research (2014)

Abstract Sym013 represents a novel HER family targeting approach consisting of a mixture of six monoclonal antibodies against EGFR, HER2, and HER3. Sym013 effectively induces rapid, simultaneous down-regulation of all HER members. Compared with single receptor or dual receptor targeting of the HER family, Sym013 provides broader inhibition and greater down-regulation efficacy. In this current study, we examined the capacity of Sym013 in combination with ionizing radiation to augment tumor response in lung (NSCLC) and head and neck (HNSCC) cancer model systems. The anti-proliferative effects of Sym013 were confirmed showing 30-60% growth inhibition across a variety of NSCLC and H&amp;N cancer cell lines that express EGFR, HER2, and/or HER3. Furthermore, Sym013 inhibited the growth of tumor cells that exhibit resistance to cetuximab. Western blot analysis showed a dose-dependent down-regulation of HER family members and downstream MAPK and AKT signaling pathways following 24-hour treatment with Sym013. Clonogenic survival analysis revealed that Sym013 enhanced radiosensitivity in tumor cells following radiation exposure. We characterized the DNA damage profile following radiation via flow cytometric analysis of phosphorylated histone 2AX (γH2AX) foci. This revealed a significant increase of γH2AX following treatment with Sym013 and 2 Gy radiation when compared with Sym013 or radiation alone. Furthermore, combined treatment of Sym013 and radiation induced a significant G1 and G2 arrest. Using Annexin V/PI staining, we also found a significant increase in the apoptotic cell population following treatment of Sym013 and 6 Gy radiation. These results suggest that Sym013 augments radiation response via the induction of cell cycle arrest followed by the induction of apoptosis and cell death, likely reflecting inhibitory effects on DNA damage repair. Additional studies to examine the impact of Sym013 to augment radiation response as well as overcome acquired resistance to cetuximab in xenograft models are in progress. Overall, these data suggest the significant capacity of Sym013 to augment radiation response in lung and head and neck cancers. This unique antibody mixture warrants additional investigation as a promising novel HER family targeting strategy in cancer therapy. Citation Format: David Francis, Shyhmin Huang, Lauryn Werner, Johan Lantto, Ivan D. Horak, Michael Kragh, Paul M. Harari. Sym013, novel pan-HER monoclonal antibody mixture, augments radiation response in human lung and head and neck tumors. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4495. doi:10.1158/1538-7445.AM2014-4495
Hamilton Bailey's Demonstrations of Physical Signs in Clinical Surgery
Authors: Alvin M. Cotlar
Journal: JAMA (1974)

The genius of English authorship is again demonstrated in Allan Clain's edition of Hamilton Bailey's textbook of physical diagnosis. This famous work, first published in 1927, is a "must" companion to Hamilton Bailey's<i>Emergency Surgery (JAMA</i>224:252, 1973). The first 50 pages cleverly introduce the reader to the basics of physical diagnosis. The text then continues with an orderly arrangement according to anatomical location. The section on the head and neck has nine individual chapters devoted to such organs as the ear, salivary glands, mouth, and thyroid, respectively. There are approximately 1,000 illustrations with the usual vivid color representations characteristic of Hamilton Bailey's books. These beautifully illustrate not only signs on inspection, but extensive techniques in palpation, often ignored in contemporary textbooks. The title, which refers to "Signs in Clinical Surgery," is misleading since the book is of quite general medical interest and value. Numerous chapters are devoted to orthopedics,
Systematic review of randomized trials on vasoconstrictor drugs for hepatorenal syndrome
Authors: Lise L. Gluud, Kurt Christensen, Erik Christensen, Aleksander Krag
Journal: Hepatology (2009)

Vasoconstrictor drugs may improve renal function in hepatorenal syndrome (HRS), but the effect on mortality has not been established. We therefore performed a systematic review of randomized trials on vasoconstrictor drugs for type 1 or type 2 HRS. Mortality was the primary outcome measure. Eligible trials were identified through electronic and manual searches. Intention-to-treat random effects meta-analyses were performed. Ten randomized trials on terlipressin alone or with albumin, octreotide plus albumin, and noradrenalin plus albumin were included. The total number of patients was 376. Overall, vasoconstrictor drugs used alone or with albumin reduced mortality compared with no intervention or albumin (relative risk [RR], 0.82; 95% confidence interval [CI], 0.70–0.96). In subgroup analyses, the effect on mortality was seen at 15 days (RR, 0.60; 95% CI, 0.37–0.97) but not at 30 days (RR, 0.74; 95% CI, 0.40–1.39), 90 days (RR, 0.89; 95% CI, 0.66–1.22), or 180 days (RR, 0.83; 95% CI, 0.65–1.05). Subgroup analyses stratified by the treatments assessed showed that terlipressin plus albumin reduced mortality compared with albumin (RR, 0.81; 95% CI, 0.68–0.97). The effect was seen in subgroup analyses of type 1 but not type 2 HRS. The remaining trials were small and found no beneficial or harmful effects of the treatments assessed. Conclusion: Terlipressin plus albumin may prolong short-term survival in type 1 HRS. The duration of the response should be considered when making treatment decisions and in the timing of potential liver transplantations. Considering the small number of patients included, the evidence does not allow for treatment recommendations regarding type 2 HRS or any of the remaining treatment comparisons assessed. (Hepatology 2009.)
Management of Ingested Foreign Bodies in Childhood
Authors: L. Spitz
Journal: BMJ (1971)

A total of 660 patients with ingested foreign bodies admitted to the general surgical services in two children9s hospitals in Liverpool are reviewed. Endoscopic removal (205 cases) is recommended for all foreign objects impacted in the oesophagus, with the exception of rounded or blunt objects in the lower third, which should be observed for a maximum of 12 hours. The indications for laparotomy for removal of a foreign body (43 cases, 6·5%) are the danger of perforation and failure of progression. The ingestion of a long slender object—for example a hair-grip—in a child under 2 years of age, is an absolute indication for prophylactic operative removal owing to the high incidence of impaction and perforation of the duodenum.
Specialty Cardiac Hospitals and Coronary Revascularization Rates
Authors: Jeffrey Stensland, Julian Pettengill, Ariel Winter, Mark Miller
Journal: JAMA (2007)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Gentamicin Dosing Errors With Four Commonly Used Nomograms
Authors: Timothy S. Lesar, John C. Rotschafer, Linda M. Strand, Lynn D. Solem, Darwin E. Zaske
Journal: JAMA (1982)

Four methods for calculating gentamicin sulfate dosage requirements were evaluated in 96 patients and compared with an individualized method. The pharmacokinetic parameters of gentamicin were determined from serum concentration time data and used to calculate individualized dosage regimens. Doses were determined in each patient using the "predictive methods" (Sarubbi-Hull, Dettli, "rule of eights," and Chan). Resultant serum concentrations were calculated from doses arrived at by each method. These dosing methods resulted in a large proportion of patients with subtherapeutic or potentially toxic concentrations, or both. The Dettli and Chan methods produced therapeutic concentrations in more patients than the Sarubbi-Hull and rule of eights methods. Desired therapeutic concentrations were attained in significantly more patients with the individualized method than with the predictive methods, and, in addition, larger doses were required. The use of predictive dosage methods should be followed with serum concentration determinations and dosage adjustment to ensure therapeutic concentrations early in treatment. (<i>JAMA</i>1982;248:1190-1193)
Anti-ischemic effects and long-term survival during ranolazine monotherapy in patients with chronic severe angina
Authors: Bernard R. Chaitman, Sandra L. Skettino, John O. Parker, Peter Hanley, Jaroslav Meluzin, Jerzy Kuch, Carl J. Pepine, Whedy Wang, Jeanenne J. Nelson, David A. Hebert, Andrew A. Wolff
Journal: Journal of the American College of Cardiology (2004)
Abstract 1435: Analyzing the role of CXCR and IGF-IR in modulating actin dynamics in neuroblastoma cells
Authors: James Muller, Chandran Sabanayagam, Cynthia M. van Golen
Journal: Cancer Research (2011)

Abstract The sensory cues that developing neurons use to control the pattering of neurite outgrowth are currently not well defined. CXCR4 and the insulin like growth factor receptor (IGF1r) have been implicated in the regulation of chemotaxis, neuronal migration, and axonal guidance. In addition to these chemokine receptors role in the guidance of neuronal development, an increasing body of evidence shows they are up-regulated in many late stage and metastatic cancers. Neuroblastoma, a cancer of neural crest origin, often expresses high levels of CXCR4 and IGF1r, and depending upon the level of differentiation, is capable of producing fully extended axons. The mediator of the cytoskeletal changes seen in axonal extension is primarily carried out by actin polymerization. In order to better understand the role of CXCR4 and IGF1r role in mediating these cytoskeletal changes we used western blots and immunocytochemistry / confocal microscopy to analyze the neuroblastoma cell line SHSY-5Y. We were able to show that indeed both receptors are involved in neuronal outgrowth. However, the individual effects of treatment with the receptors ligand resulted in different cellular morphologies. CXCR4 stimulation resulted in a more differentiated neuronal form, while IGF1r stimulation resulted in a very immature neuronal morphology with shorter broader processes. Finally, we were able to quantitate the effects of treatment to a wide array of phenotypic patterns that shows the connection between our observed morphological changes to show that these observations are real effects of treatment. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1435. doi:10.1158/1538-7445.AM2011-1435
De novo synthesis and secretion of a 36-kD protein by cells that form lupus inclusions in response to alpha-interferon.
Authors: S A Rich
Journal: Journal of Clinical Investigation (1995)

In response to the pure recombinant human alpha-IFN, IFLrA, Raji and Daudi were the only two cell lines among 19 human lymphoblastoid cell lines tested that formed the human lupus inclusions (LI) to a high frequency. Raji, Daudi, and five other cell lines were examined for protein changes that might accompany LI formation. Their selection was based upon T or B origin, association with Epstein-Barr virus, and ability to form LI. A trace protein of an estimated molecular mass of 36 kD (p36) and an isoelectric point of 5.6 was detected on two-dimensional gels only of alpha-IFN-treated Raji and Daudi cells. Gamma-IFN did not induce p36 or LI in any of these seven cell lines. In Daudi cells p36 and LI formed simultaneously in response to IFLrA, and persisted until the alpha-IFN-induced death of the culture. In Raji cells, p36 and LI appearance and disappearance coincided with the addition and removal of alpha-IFN. Fractionation of Raji cells with nonionic-detergent buffer placed p36 with the inclusions in the cytoplasmic supernatant. With detergent-free buffer p36 and LI were distributed evenly between the nuclear and cytoplasmic fractions. Pulse-chase experiments revealed that p36 was secreted. The de novo synthesis of p36 with alpha-IFN treatment was shown by labeling the cell proteins with [35S] methionine before and after the addition of alpha-IFN. These results along with previous results on the de novo synthesis of LI in the endoplasmic reticulum (which is involved in the processing and secretion of proteins) suggest a role for LI in the synthesis and secretion of p36.
Serum fibroblast growth factor 21 levels in gestational diabetes mellitus in relation to insulin resistance and dyslipidemia
Authors: Sebastian Stein, Holger Stepan, Jürgen Kratzsch, Michael Verlohren, Hans-Joachim Verlohren, Kathrin Drynda, Ulrike Lössner, Matthias Blüher, Michael Stumvoll, Mathias Fasshauer
Journal: Metabolism (2009)