tbuckley/cabot-search huggingface dataset:
Successful Immunization with a Single Injection of Non-integrating Lentiviral Vector
Authors: Donatella RM Negri, Zuleika Michelini, Silvia Baroncelli, Massimo Spada, Silvia Vendetti, Viviana Buffa, Roberta Bona, Pasqualina Leone, Mary E Klotman, Andrea Cara
Journal: Molecular Therapy (2007)

We evaluated the ability of an integrase (IN)-defective self-inactivating lentiviral vector (sinLV) for the delivery of human immunodeficiency virus-1 (HIV-1) envelope sequences in mice to elicit specific immune responses. BALB/c mice were immunized with a single intramuscular injection of the IN-defective sinLV expressing the codon optimized HIV-1(JR-FL) gp120 sequence, and results were compared with those for the IN-competent counterpart. The IN-defective sinLV elicited specific and long-lasting immune responses, as evaluated up to 90 days from the immunization by enzyme-linked immunosorbent spot (ELISPOT) and intracellular staining (ICS) for interferon-gamma (IFN-gamma) assays in both splenocytes and bone marrow (BM) cells, chromium release assay in splenocytes, and antibody detection in sera, without integration of the vector into the host genome. These data provide evidence that a single administration of an IN-defective sinLV elicits a significant immune response in the absence of vector integration and may be a safe and useful strategy for vaccine development.
BCG Vaccine Abscesses Are Unrelated to HIV Infection
Authors: Robert L. Colebunders
Journal: JAMA (1988)

<h3>To the Editor.</h3> —Concern has been expressed about the administration of live vaccines to young children with human immunodeficiency virus (HIV) infection.<sup>1</sup>There is a theoretical possibility that these children will have an adverse response to these vaccines (ie, to oral poliovirus, measles virus, and BCG vaccines). We were therefore concerned when, between September and December 1986, we noted an outbreak of axillary and epicondylar abscesses in children in Kinshasa vaccinated with BCG vaccine. We wondered if the development of these abscesses had been favored by an underlying HIV infection in the vaccinated children. <h3>Study.</h3> —All children vaccinated with BCG vaccine presenting with abscesses at Mama Yemo Hospital during October and November 1986 were screened for HIV antibodies using an enzyme-linked immunosorbent assay. Nineteen children (ten boys and nine girls) aged from 2 to 7 months were enrolled in the study. Abscesses were axillary and/or epicondylar and were localized
Effect of Permethrin–Impregnated Underwear on Body Lice in Sheltered Homeless Persons
Authors: Samir Benkouiten, Rezak Drali, Sékéné Badiaga, Aurélie Veracx, Roch Giorgi, Didier Raoult, Philippe Brouqui
Journal: JAMA Dermatology (2013)

The control of body lice in homeless persons remains a challenge.To determine whether the use of long-lasting insecticide-treated underwear provides effective long-term protection against body lice in homeless persons.A randomized, double-blind, placebo-controlled trial was conducted in February and December 2011 in 2 homeless shelters (Madrague Ville and Forbin) in Marseille, France. Of the 125 homeless persons screened for eligibility, 73 body lice-infested homeless persons, 18 years or older, were enrolled.Body lice-infested homeless persons were randomly assigned to receive 0.4% permethrin-impregnated underwear or an identical-appearing placebo for 45 days, in a 1:1 ratio, with a permuted block size of 10. Visits were scheduled at days 14 and 45. Data regarding the presence or absence of live body lice were collected.The primary and secondary end points were the proportions of homeless persons free of body lice on days 14 and 45, respectively. Mutations associated with permethrin resistance in the body lice were also identified.Significantly more homeless persons receiving permethrin-impregnated underwear than homeless persons receiving the placebo were free of body lice on day 14 in the intent-to-treat population (28% vs 9%; P = .04), with a between-group difference of 18.4 percentage points (95% CI, 1.4-35.4), and in the per-protocol population (34% vs 11%; P = .03), with a between-group difference of 23.7 percentage points (95% CI, 3.6-43.7). This difference was not sustained on day 45. At baseline, the prevalence of the permethrin-resistant haplotype was 51% in the permethrin group and 44% in the placebo group. On day 45, the permethrin-resistant haplotype was significantly more frequent in the permethrin group than in the placebo group (73% vs 45%, P < .001).Permethrin-impregnated underwear is more efficient than placebo at eliminating body louse infestations by day 14; however, this difference was not sustained on day 45. The use of permethrin may have increased the resistance to permethrin in body lice and thus must be avoided.clinicaltrials.gov Identifier: NCT01287663.
Hybridization of the Primary Care Disciplines
Authors: Norman D. Ferrari
Journal: JAMA (1986)

To the Editor.—  The articles and editorials in your May 16,1986, issue dealing with a better way to train primary care physicians fascinate me. 1-4 The authors of one article proposed a four-year combined training program in family practice and internal medicine, so that the strengths and weaknesses of each field might complement and enhance the other. 1   There are already in the United States more than 75 programs offering a combined four-year residency leading to potential board certification in two primary disciplines—internal medicine and pediatrics. 5 These programs were alluded to in Dr Geyman's 2 option 2. The program I have personally been affiliated with for the last four years has a primary care emphasis and is partially funded by a federal grant designed to train primary care physicians. The combined medicine/pediatric residency program at West Virginia University (and our sister programs across the country) has experienced increasing growth
Blood markers of early and late airway responses to allergen in asthmatic subjects. Relationship with functional findings
Authors: L Bancalari, F. L. Dente, S. Cianchetti, C. Prontera, M. Taccola, E. Bacci, A. Carletti, A. Di Franco, D. Giannini, B. Vagaggini, M. Ferdeghini, P. L. Paggiaro
Journal: Allergy (1997)

We evaluated the relationship between blood markers of mast‐cell (plasma histamine and serum level of heat‐stable neutrophil chemotactic activity [NCA]) and eosinophil (serum eosinophil cationic protein [ECP]) activation during early airway response (EAR) and late airway response (LAR) to allergen inhalation in 24 asthmatic subjects. After EAR, 14 subjects showed significant LAR (FEV 1 fall: 25%), while 10 subjects showed equivocal LAR (FEV 1 fall: 15–20%). A significant increase from baseline value was observed in plasma histamine and in serum NCA during both EAR and LAR, while serum ECP significantly increased only during LAR. The sensitivity of different markers to detect significant FEV 1 fall during EAR and LAR was low, except for NCA. Changes in blood mediators were similar in both groups with significant and equivocal LAR. There was a significant relationship between the increase in NCA during EAR and the severity of LAR. Stepwise regression between changes in different blood markers showed a significant relationship between histamine increase during EAR and ECP increase during LAR. Thus, serum NCA is a more sensitive marker of EAR and LAR than plasma histamine and serum ECP, and its increase during EAR seems predictive of the severity of the subsequent LAR.
Coproporphyrin I and III excretion in bile and urine
Authors: Neil Kaplowitz, Norman Javitt, Attallah Kappas
Journal: Journal of Clinical Investigation (1972)

The excretion of coproporphyrin isomers I and III was studied in the rat. Both isomers were found to bind equally to rat plasma and liver cytosol in vitro and to disappear from plasma at equal rates after single injections in vivo. During equimolar infusions of isomers into bile fistula animals, both the I and III isomers were excreted in bile in a concentration ratio of 2:1, respectively. Pretreatment of animals with ethinylestradiol or simultaneous infusions of phenoldibromophthalein disulfonate caused a reduction in total hepatic excretion with no change in the 2:1 ratio in bile. As hepatic excretion fell, excretion of both isomers in urine rose, with an increase in the proportion of the I isomer. The findings mimic those reported to occur in man and can be explained by inhibition of a common carrier which requires a stereospecific configuration that statistically favors the hepatic transport of the symmetrical coproporphyrin I isomer.
Midline Catheterization in Hospitalized Patients
Authors: Abba I. Terr
Journal: Annals of Internal Medicine (1996)

Letters15 October 1996Midline Catheterization in Hospitalized PatientsAbba I. Terr, MD and Daniel A. Bloch, PhDAbba I. Terr, MDStanford University Medical Center, Stanford, CA 94305Search for more papers by this author and Daniel A. Bloch, PhDStanford University Medical Center, Stanford, CA 94305Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-125-8-199610150-00017 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:Mermel and colleagues [1] encountered two unexpected adverse events in 238 patients in whom Landmark catheters had been inserted, and they concluded that a toxin or allergen was responsible. In the accompanying editorial, Maki [2] interpreted these events as anaphylactoid reactions to elastomeric hydrogel.As consultants to Menlo Care, the manufacturer of Landmark catheters, we reviewed all 140 adverse events reported to the company through early 1995, including those observed by Mermel and colleagues. The conclusions of Mermel and colleagues are unsubstantiated. Their two cases involved no urticaria, angioedema, bronchospasm, hypotension, or laryngeal edema. Symptoms resolved within ...References1. Mermel LA, Parenteau S, Tow SM. The risk of midline catheterization in hospitalized patients. A prospective study. Ann Intern Med. 1995; 123:841-4. Google Scholar2. Maki DG. Reactions associated with midline catheters for intravenous access [Editorial]. Ann Intern Med. 1995; 123:884-6. Google Scholar3. Pavlin DJ, Links S, Rapp SE, Nessly ML, Keyes HJ. Vasovagal reactions in an ambulatory surgery center. Anesth Analg. 1993; 76:931-5. Google Scholar4. Koritos HA, Richardson DW, Raper AJ, UI-Hassan Z, Patterson JL. Mechanisms of action of hypocapnic alkalosis on limb blood vessels in man and dog. Am J Physiol. 1972; 223:1296-307. Google Scholar5. Block M, Szidon P. Hyperventilation syndromes. Comprehensive Therapy. 1994; 20:306-11. Google Scholar Author, Article, and Disclosure InformationAffiliations: Stanford University Medical Center, Stanford, CA 94305 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byRe‐evaluating expanding intravenous catheters in medical practice 15 October 1996Volume 125, Issue 8Page: 696KeywordsAdverse eventsAllergy and immunologyCathetersNauseaPatientsRandomized trialsSyncopeToxinsUrticariaVomiting Issue Published: 15 October 1996 Copyright & PermissionsCopyright © 1996 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
A Case of Gastric Fistula: Operation: Death
Authors: C. F. M. Althorp
Journal: BMJ (1901)

THE following case is of interest because it raises the ques- tion of the surgical treatment of hiematemesis due to gastric ulcer.S. S., aged i9, a domestic servant, vas admiiitted into the Newcastle Royal Infirnmary on Decem-ber x5th, I900, suffering from hnematemesis.History.-She had suffered from epigastric pain, coming on two hours
MicroRNA-mediated loss of ADAR1 in metastatic melanoma promotes tumor growth
Authors: Yael Nemlich, Eyal Greenberg, Rona Ortenberg, Michal J. Besser, Iris Barshack, Jasmine Jacob-Hirsch, Elad Jacoby, Eran Eyal, Ludmila Rivkin, Victor G. Prieto, Nitin Chakravarti, Lyn M. Duncan, David M. Kallenberg, Eitan Galun, Dorothy C. Bennett, Ninette Amariglio, Menashe Bar-Eli, Jacob Schachter, Gideon Rechavi, Gal Markel
Journal: Journal of Clinical Investigation (2013)

Some solid tumors have reduced posttranscriptional RNA editing by adenosine deaminase acting on RNA (ADAR) enzymes, but the functional significance of this alteration has been unclear. Here, we found the primary RNA-editing enzyme ADAR1 is frequently reduced in metastatic melanomas. In situ analysis of melanoma samples using progression tissue microarrays indicated a substantial downregulation of ADAR1 during the metastatic transition. Further, ADAR1 knockdown altered cell morphology, promoted in vitro proliferation, and markedly enhanced the tumorigenicity in vivo. A comparative whole genome expression microarray analysis revealed that ADAR1 controls the expression of more than 100 microRNAs (miRNAs) that regulate many genes associated with the observed phenotypes. Importantly, we discovered that ADAR1 fundamentally regulates miRNA processing in an RNA binding–dependent, yet RNA editing–independent manner by regulating Dicer expression at the translational level via let-7. In addition, ADAR1 formed a complex with DGCR8 that was mutually exclusive with the DGCR8-Drosha complex that processes pri-miRNAs in the nucleus. We found that cancer cells silence ADAR1 by overexpressing miR-17 and miR-432, which both directly target the ADAR1 transcript. We further demonstrated that the genes encoding miR-17 and miR-432 are frequently amplified in melanoma and that aberrant hypomethylation of the imprinted DLK1-DIO3 region in chromosome 14 can also drive miR-432 overexpression.
Cost Containment and the Physician
Authors: Marcia Angell
Journal: JAMA (1985)

The rapid rise in health care costs is receiving a good deal of attention these days. Proposed responses include the deliberate rationing of expensive medical technologies, such as organ transplantation, and a redirection of our efforts toward preventive care. Although preventive care may improve our health, it cannot be assumed to reduce medical costs, since a later death may be as expensive as an earlier one. I suggest that a major and rapidly growing component of medical costs stems from the widespread application of tests and procedures when they are of no demonstrated benefit and may even be harmful. Identifying and curtailing such unnecessary medical care, rather than rationing beneficial technologies, should be the thrust of cost-containment efforts. Fee schedules should be revised so that they neither encourage nor discourage the use of tests and procedures; we should undertake systematic studies to assess technologies and practices; and we should make every effort to discourage the practice of defensive medicine. The involvement of physicians in rationing is not only premature; it is also inconsistent with our role as advocates for the health of our patients.Angell, senior deputy editor of the New England Journal of Medicine, rejects controlling health care costs either by the rationing of expensive medical technologies or by the redirection of efforts toward preventive care that may only postpone expenditures. She suggests that a major component of health care costs is consumed by nonbeneficial, even harmful, procedures. Identifying, assessing, and curtailing of such procedures by neutrality in fee schedules and by discouraging the practice of defensive medicine should be the thrust of cost containment efforts. Physicians are urged to reject involvement in rationing as inconsistent with their role as patient advocates and to support technology assessment, fee revisions, and more stringent self regulation as ways to discourage malpractice suits.
Barium block of Kir2 and human cardiac inward rectifier currents: evidence for subunit-heteromeric contribution to native currents
Authors: G SCHRAM, M POURRIER, Z WANG, M WHITE, S NATTEL
Journal: Cardiovascular Research (2003)

Kir2 subunits are believed to underlie the cardiac inwardly rectifying current I(K1). The subunit composition of native I(K1) currents is uncertain, and it has been suggested that heteromultimer formation may play a role.We studied Ba(2+) block of homo- and heteromeric Kir2 channels in Xenopus oocytes and compared the properties observed to those of human cardiac I(K1) in cells isolated from myocardial biopsies of normal human hearts.Homomeric expression of Kir2.1 and Kir2.3 produced currents with similar Ba(2+) sensitivities (e.g. IC(50) at -120 mV: 16.2+/-3.4, n=11 and 18.5+/-2.1, n=10, respectively), but these were less sensitive to Ba(2+) than native I(K1) (4.7+/-0.5 microM, n=10, P=0.001, P<0.001, respectively) and had different Ba(2+) blocking kinetics from cardiac I(K1). Kir2.2 sensitivity was similar to cardiac I(K1) (e.g., 2.8+/-0.4 microM, Kir2.2, n=9, vs. 4.7+/-0.5 microM for I(K1)), but the blocking kinetics of Kir2.2 were faster than those of I(K1). Currents resulting from co-expression of Kir2 subunits had similar Ba(2+) sensitivities and blocking kinetics among groups and were similar to I(K1) in both Ba(2+) sensitivity (e.g., IC(50) at -120 mV: 4.5+/-1.0, 2.5+/-0.5, and 2.3+/-0.4 microM for co-injected Kir2.1/2.2, n=6, Kir2.1/2.3, n=5, and Kir2.2/2.3, n=4, respectively) and blocking kinetics.Co-injection of Kir2 subunits results in currents with Ba(2+) blocking properties different from homomeric Kir2 expression but similar to cardiac I(K1). These observations suggest that a substantial proportion of native I(K1) may result from heteromultimer formation among diverse Kir2 family subunits.
Flt3 tandem duplication mutations cooperate with Wnt signaling in leukemic signal transduction
Authors: Lara Tickenbrock, Joachim Schwäble, Markus Wiedehage, Björn Steffen, Bülent Sargin, Chunaram Choudhary, Christian Brandts, Wolfgang E. Berdel, Carsten Müller-Tidow, Hubert Serve
Journal: Blood (2005)
Thrombocytosis in Multiple Myeloma
Authors: ABRAHAM P. ZIMELMAN
Journal: Annals of Internal Medicine (1973)

Letters1 June 1973Thrombocytosis in Multiple MyelomaABRAHAM P. ZIMELMAN, M.D.ABRAHAM P. ZIMELMAN, M.D.Author, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-78-6-970 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: In multiple myeloma the platelet count is usually normal or decreased (1). Thrombocytosis is unusual. This report describes three cases of multiple myeloma associated with thrombocytosis.Patient 1, a 63-year-old man, presented with a 24-hour history of shaking chills. On physical examination he had a temperature of 38.9 °C [102 °F], rales in both bases, and an apical systolic murmur. Laboratory studies showed the following values: hemogloblin, 7.6 g/100 ml; hematocrit, 22; leukocyte count, 15 000/mm3, with a normal differential; and platelet count, 800 000/mm3(2). Blood smear showed rouleau formation and increased platelets (Figure 1A). Serum...References1. WINTROBE M: Clinical Hematology, 6th ed., Philadelphia, Lea & Febiger, 1967, pp. 1191-1192 Google Scholar2. REESECKER HE: An improved method for counting blood platelets. JAMA 80:621-622, 1923 CrossrefGoogle Scholar3. MOROWITZALLENKIRSNER DLJ: Thrombocytosis in chronic inflammatory bowel disease. Ann Intern Med 68:1013-1021, 1968 LinkGoogle Scholar4. CROSBY W: Thrombocytosis, in The Platelet. Monograph no. 11 in International Academy of Pathology Series. Edited by BRINKHOUS KM, SHERMER RW, MOSTOFI FK. Baltimore, The Williams & Wilkins Co., 1971, pp. 309-314 Google Scholar5. WALDENSTRÖM J: Diagnosis and Treatment of Multiple Myeloma. New York, Grune & Stratton, 1970, p. 70 Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Department of Medicine Veterans Administration Hospital Boston, Massachusetts PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByEssential Thrombocythemia and Multiple Myeloma: Two Rare Diseases in One PatientPseudoerythrocytosis in myeloma with associated peripheral neuropathy.LiteraturverzeichnisImmunoglobulin D Multiple Myeloma with ThrombocytosisJOHN E. NESTLER, M.D.Thrombocytosis, coronary thrombosis and acute myocardial infarctionCANCER OF GALLBLADDER WITH SEVERE THROMBOCYTOSISBiology, Clinical Patterns, and Treatment of Multiple Myeloma and Related Plasma–Cell DyscrasiasCase 44-1977Thrombocythaemia and Multiple MyelomaPlättchenfunktion bei Paraproteinosen 1 June 1973Volume 78, Issue 6Page: 970-971KeywordsBloodHematocritMultiple myelomaPlateletsResearch laboratoriesSystolic ejection murmurTemperatureThrombocytosis Issue Published: 1 June 1973 PDF DownloadLoading ...
Birth in the ER
Authors: Bonnie Salomon
Journal: Annals of Internal Medicine (1993)

Ad Libitum1 January 1993Birth in the ERBonnie Salomon, MDBonnie Salomon, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-118-1-199301010-00015 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Grins around the roomfrom tough and weathered nurseswho “plug in” the nursing homers,bloodied drunks, assorted comersto this place of first resort.Congratulationsfrom the doctor,words not used here—ever—in this place of panicover decay and brutality.The baby comes quickly,ruptured membranes and splash,the gush of rich fluids.Hardly time to open the OB set-up.Hardly time at all.Behind other curtainssit the wounded and scarred.Infirmities untreated, losttime with lost looks.“Waiting for labs …”“Waiting for a bed”.“Hanging antibiotics now”.They only heard the screams of birth.Infuse hope and health into them(if only, if only)they absorbed the grins around this room. Author, Article, and Disclosure InformationAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 January 1993Volume 118, Issue 1Page: 75KeywordsAntibioticsBirth ePublished: 15 August 2000 Issue Published: 1 January 1993 Copyright & PermissionsCopyright © 1993 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Low dose BMP-2 treatment for bone repair using a PEGylated fibrinogen hydrogel matrix
Authors: Dror Ben-David, Samer Srouji, Keren Shapira-Schweitzer, Olga Kossover, Eran Ivanir, Gisela Kuhn, Ralph Müller, Dror Seliktar, Erella Livne
Journal: Biomaterials (2013)
IL-18 deficiency selectively enhances allergen-induced eosinophilia in mice
Authors: Taku Kodama, Tomohiro Matsuyama, Kozo Kuribayashi, Yasuhiro Nishioka, Minoru Sugita, Shizuo Akira, Kenji Nakanishi, Haruki Okamura
Journal: Journal of Allergy and Clinical Immunology (2000)
THE IDENTITY OF NERVE FORCE AND ELECTRICITY.
Authors: J. EMMET O'BRIEN
Journal: JAMA (1903)

There has been in recent years such advance by physiologists in knowledge of nerve mechanisms, though not of nerve force, and such advance by electricians in knowledge of electricity in its protean phases, that, I think, the view of the relation of nerve force to electricity ought to be taken up anew by physiologists and restudied in the light of modern electricity. Advances by physiologists appear in minutely studied anatomy of neurons, relaying nerve mechanisms, conduction paths, terminal mechanisms, etc., and advances in this direction by electricians have been in better knowledge of the electric phenomena of cables, learned since physiologic views of the electrical phenomena of nerves became rather arbitrarily fixed; of fine currents and the wide field of induction, as revealed by the telephone; new light on the merely relative value of insulation; the phenomena of high frequency currents and their apparent reversal of electric law in insulation;