tbuckley/cabot-search huggingface dataset:
Honeybee venom allergy: immunoblot studies in allergic patients after immunotherapy and before sting challenge
Authors: S. Jeep, M. Paul, U. Müller, G. Kunkel
Journal: Allergy (1996)

By immunoblot techniques, detailed antibody studies were performed with sera of 20 honeybee-venom-allergic patients during or at the end of specific immunotherapy (median duration: 3 years) and before honeybee sting challenge. Before immunotherapy, all patients had experienced systemic allergic reactions to a honeybee sting, with a mean severity of 3.5 +/- 0.5 according to the Müller classification. After the sting challenge, 10 patients (reactors) reacted again with a systemic allergic reaction, whereas 10 patients (nonreactors) did not. No differences were observed between reactors and nonreactors in total serum IgE and specific IgE to honeybee venom at the time of challenge. For immunoblot, honeybee venom (RELESS) was separated on 7.5-20% SDS-PAGE. For detection of specific IgE, IgG, IgG1, IgG4, and IgM, an alkaline phosphatase-linked second antibody was used. Both groups showed 11 antibody-binding bands: at 52, 46, 40, 31, 18.7, 16.9, 13, 11, 10, 9, and 8 kDa; however, the antibody-binding pattern was individual. The reactors differed from nonreactors in showing intense IgE and less IgG4 binding to at least one single component of the venom extract. For nonreactors, the inverse relationship was observed. The hypothesis, "intensity of IgE > or = IgG4 leads to allergic symptoms", was highly significant (P = 0.00026; chi-square). These immunoblot findings could offer predictive value in distinguishing reactors from nonreactors.
KIR and HLA‐Cw genotypes of donor‐recipient pairs influence the rate of CMV reactivation following non‐T‐cell deleted unrelated donor hematopoietic cell transplantation
Authors: Xiaojin Wu, Jun He, Depei Wu, Xiaojing Bao, Qiaocheng Qiu, Xiaoni Yuan, Yue Han, Ainig Sun, Guanghua Chen, Yang Xu
Journal: American Journal of Hematology (2009)
The Prevalence and Mortality of Cancer
Authors: Henry Power
Journal: JAMA (1913)

VOLit.AfE LX NUAI13EP.I CORRPSPOA7DE.ATCE 67 fundamental changes in the conduct of health departments, both inullicipal and state, and to clearer ideas regarding the 8Phere Of usefulness of leading foreign health resorts.It is also confidently believed that not only will the advantages offered be greater, but, if properly conducted, the cost pOer c4Pita Will be much less than -if a, single individual were to make a similar tour.The organization would, therefore, work to the decided 46irantage of the individual members of the profession.
Peer Review and the NCI's Clinical Alert on Node-Negative Breast Cancer
Authors: B. A. Feinberg
Journal: JAMA (1989)

<h3>To the Editor.—</h3> On May 16, 1988, the National Cancer Institute (NCI) released a media statement in conjunction with a letter to 13 000 cancer specialists regarding new recommendations on adjuvant therapy for breast cancer. The letter, entitled "NCI Clinical Alert Urgent," heralds a major change in longstanding policy, recommending adjuvant systemic therapy for node-negative breast cancer.<sup>1</sup> The unorthodox method by which this information was disseminated has been explained as an effort to reach physicians quickly rather than waiting months for the data from the three referenced studies to be published. However, the NCI was unusually quiet in releasing the information to the public, choosing to rely on a mailed press release rather than a formal news conference. Furthermore, the mailing to cancer specialists disregards the fact that most women with node-negative breast cancer are evaluated solely by their primary care physician and general surgeon. There are several inconsistencies
Abstract 2207: SUMOylation of EAPII/TTRAP/TDP2 modulates its function
Authors: Chunyang Li, Fadlo R. Khuri, Shi-yong Sun, Runzhao Li
Journal: Cancer Research (2012)

Abstract ETS1 Associated Protein II (EAPII, also called TTRAP, TDP2) was originally identified by two-hybrid screening as a novel protein that interacts with both ETS1 and the cytoplasmic domain of CD40 receptor. EAPII may represent a novel component in signal transduction processes that regulates eukaryotic gene expression in response to extracellular signals. In support of this hypothesis, we have demonstrated that EAPII negatively modulates ETS1 transcriptional activity and attenuates synergistic transactivation by ETS1 and NFκB. However, the mechanism by which EAPII transduces signals is elusive. In this report, we provide the evidence of EAPII protein sumoylation in vivo. Sumoylation is an important posttranslational modification that involves conjugation of the small ubiquitin-like modifier (SUMO) to lysine residues of the targeting proteins. EAPII is sumoylated by both SUMO-1 and SUMO-2/3. Sumoylation analysis of cell fractions revealed that sumoylation by SUMO-2/3 mainly occurs in the nucleus while that by SUMO-1 in cytoplasm. Since EAPII protein possesses no SUMO consensus motif (≥KxE), we constructed a series of EAPII truncated mutations to determine the potential sumoylation site(s). Our data indicated that the noconsensus sumoylation site(s) may reside between amino acid residues 68 and 279. Interestingly, C-terminal deletion (Del 279-362) significantly increases the sumoylation, suggesting that the C-terminus might contain an inhibitory domain for EAPII sumoylation. It has been hypothesized that sumoylation may mediate extracellular signals in response to stresses, hypoxia and cytokines. Whether EAPII sumoylation interferes with its repressive activity on ETS1 transcription remains to be elucidated, and determination of the signaling pathways that may modulate EAPII sumoylation will provide a better understanding of the modulation of ETS1 functions in specific pathphysiological microenvironments. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2207. doi:1538-7445.AM2012-2207
Temporal relationship between hepatitis B virus enhancer II/basal core promoter sequence variation and risk of hepatocellular carcinoma
Authors: Y-C Chou, M-W Yu, C-F Wu, S-Y Yang, C-L Lin, C-J Liu, W-L Shih, P-J Chen, Y-F Liaw, C-J Chen
Journal: Gut (2007)

<h3>Background and aims:</h3> To investigate the temporal relationship between sequence variation in the enhancer II (EnhII), basal core promoter (BCP), and precore regions of hepatitis B virus (HBV) and the risk of hepatocellular carcinoma (HCC), we conducted a nested case–control study within a cohort of 4841 male HBV carriers who were recruited during the period 1988–1992. <h3>Methods:</h3> The HBV DNA sequence was determined in baseline blood samples taken from 132 incident cases and 204 controls. Base exchanges during follow-up in 71 cases were compared with 81 controls with samples taken during a similar length of follow-up. <h3>Results:</h3> Nine single nucleotide polymorphisms in the EnhII/BCP regions (six of which were genotype C HBV related) were associated with subsequent risk of HCC. The strength of these associations decreased as the lag time between baseline measurement and diagnosis increased over 3 years. However, an increased disease risk in subjects with BCP double variants (mostly T1762/A1764) or genotype C HBV-related variants was evident 9 years or more before diagnosis. The BCP double variants (odds ratio, 1.92 (95% confidence interval, 1.14 to 3.25)) were statistically significantly associated with HCC risk even after adjusting for alanine aminotransferase levels, antibodies against HBV e antigen, HBV genotype, HBV viral load, and other sequence variants. Longitudinal analysis indicated that the increased HCC risks for at-risk sequence variants were attributable to the persistence of these variants. <h3>Conclusions:</h3> HCC risk is associated with sequence variation in the EnhII/BCP regions of HBV, and persistence of at-risk sequence variants is critical for HCC development.
Temporal regulation of endothelial ET-1 and eNOS expression in intact human conduit vessels exposed to different intraluminal pressure levels at physiological shear stress
Authors: Li-ming Gan, Lena Selin-Sjögren, Roya Doroudi, Sverker Jern
Journal: Cardiovascular Research (2000)

By using a computerized vascular perfusion model, we investigated temporal effects of sub-acute pressure elevation on vasomotor behavior and expression of endothelin-1 (ET-1) and endothelial nitric oxide synthase (eNOS) in intact human conduit vessels.Paired umbilical veins were perfused during 1.5, 3 and 6 h under high/low intraluminal pressure (40/20 mmHg) and at identical shear stress level of 10 dyn/cm(2). ET-1 and eNOS gene and protein expression was quantified with real-time reverse-transcribed polymerase chain reaction and quantitative immunohistochemistry, respectively.Pressure induced differential temporal regulation patterns of ET-1 and eNOS gene expression. During the high pressure condition, eNOS mRNA was upregulated after 3 h and leveled off after 6 h of perfusion, while ET-1 mRNA was elevated after 6 h perfusion. Immunohistochemistry verified synchronal changes at the protein level. Significant vasodilation was observed after 3 h in the high-pressure system.Thus, subacute pressure elevation exerts differential effects on the endothelial eNOS/ET-1 expression, which dynamically regulate the vasomotor tone.
Sgk: an old enzyme revisited
Authors: Nicolette Farman, Sheerazed Boulkroun, Nathalie Courtois-Coutry
Journal: Journal of Clinical Investigation (2002)

The elucidation of a key role for the serum- and glucocorticoid-regulated kinase (Sgk) in the regulation of the epithelial Na+ channel (ENaC) has led to substantial advances in the understanding of the signaling pathways involved in corticosteroid hormone action (1, 2). Indeed it is well established that the mineralocorticoid hormone aldosterone is a major regulator of body fluid homeostasis and blood pressure levels, mainly because of its capacity to modulate renal sodium reabsorption. However many aspects of this regulation pathway are not fully understood. Aldosterone binds to the mineralocorticoid receptor (MR), a member of the nuclear receptor superfamily which controls a network of genes in tight epithelia, ultimately leading to regulation of sodium entry into epithelial cells via the amiloride-sensitive ENaC (3). Most of the early steps of aldosterone action following MR-mediated transcriptional events are still incompletely elucidated. Differential hybridization studies have identified the serine/threonine kinase Sgk as a major transcriptional target of aldosterone. At least two other isoforms of Sgk, Sgk2 and Sgk3, have been identified after the initial characterization of Sgk (1), now referred to as Sgk1 (4). In this issue of the JCI, Wulff and colleagues (5) generated sgk1-deficient mice and examined the effects of dietary salt-restriction on renal water and electrolyte excretion.
Induction of metallothionein and its localization in the nucleus of rat hepatocytes after partial hepatectomy
Authors: Chiharu Tohyama, Junko Sayato Suzuki, Jack Hemelraad, Noriko Nishimura, Hisao Nishimura
Journal: Hepatology (1993)

Metallothioneins, a group of cysteine-rich heavy-metal binding proteins, are induced in the regenerating rat liver in response to the stimuli evoked by partial hepatectomy. We have investigated the expression of metallothionein genes and proto-oncogenes (c-fos, c-jun and c-myc), as well as specific localization of metallothionein in the liver cells after partial hepatectomy. Metallothionein mRNA was detected as early as 3 hr and reached a maximal level by 6 hr. Expression of the proto-oncogenes apparently preceded the elevation of metallothionein protein because the latter was maximal 18 hr after partial hepatectomy, followed by a decrease until 70 hr. Hepatocytes of the intact rat liver have metallothionein in the cytoplasm only. Interestingly, metallothionein was localized predominantly in the nucleus as early as 6 hr after partial hepatectomy, and the staining intensity of metallothionein became maximal at 15 hr, followed by detection in both the cytoplasm and nucleus at 24 hr or longer. The use of a confocal laser scanning microscope with both tissue sections and isolated nuclei has clearly shown that metallothionein immunofluorescence exists inside hepatocyte nuclei after partial hepatectomy. Expression of the proto-oncogenes c-fos and c-jun is elevated after partial hepatectomy, and the resultant heterodimer of gene products may contribute to the observed metallothionein gene induction. However, the observation that metallothionein protein levels were elevated until 18 hr after partial hepatectomy suggests that an alternative pathway for the induction of metallothionein gene expression may also be present.(ABSTRACT TRUNCATED AT 250 WORDS)
Points: Thesis by questionnaire
Authors: J P Lester
Journal: BMJ (1984)

This described both the numerals themselves atid their use in calculation.Al-Khwarizmi's book was translated into Latin by an Englishrman, Robert of Chester, in 1120.This was the first of several Latin translations by means of which the Hindu-Arabic numerals became known to Western scholars.The methods of calculation using these numerals were given the general heading, algorithmus, from which our word "algorithm" is derived.Al- Khwarizmi's name has thus been preserved for us, in effect through a confusion between the name of the methods and the author of the principal work through which they became known.Algorithmus is just a corruption of the name Al-Khwarizmi.
Qa-1 restriction of CD8+ suppressor T cells
Authors: Stefanie Sarantopoulos, Linrong Lu, Harvey Cantor
Journal: Journal of Clinical Investigation (2004)

There is increasing evidence that the immune response can be inhibited by several T cell subsets, including NK T cells, CD25+CD4+ T cells, and a subpopulation of CD8+ T cells. Animal model studies of multiple sclerosis have suggested an important role for suppressor CD8+ T cells in protection against disease recurrence and exacerbation. The molecular lynchpin of CD8+ suppressive activity is the murine MHC molecule Qa-1, termed HLA-E in humans. Here we summarize findings from work on Qa-1 that have begun to delineate suppressor CD8+ T cells and their mechanisms of action in the context of self tolerance and autoimmune disease.
Determination of Serum Creatinine Level Prior to Administration of Radiographic Contrast Media
Authors: Sachiko T. Cochran
Journal: JAMA (1997)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Lithium and motor vehicle crashes
Authors: Tom R Dening
Journal: BMJ (2004)

Research on preventing road traffic injuries in developing countries is neededEditor-The dedication of the World Health Organization's World Health Day 2004 to road safety is recognition of the global threat of road traffic injuries.Increasing population size, vehicle ownership, road infrastructure, and transnational transportation have created a substantial public health burden.However, little epidemiological research has been conducted into preventing road traffic injuries in developing countries.Currently global research and development funding per disability adjusted life year for HIV, asthma, and blindness are $26.2,$10.8, and $5.4, respectively, while only $0.40 for road traffic injuries. 1 Epidemiological studies of successful interventions preventing road traffic injuries have been conducted in developed countries. 2But to assume the same effectiveness in developing countries is inappropriate because of behavioural, vehicular, and environmental differences.A pooled analysis of street lighting intervention data from developed countries shows a protective effect, 3 particularly in pedestrian injury (relative risk 0.56), the most common road traffic injuries in developing countries.Annually, 1.2 million deaths are attributed to road traffic crashes, 85% occurring in developing countries. 4If an intervention such as street lighting could reduce deaths by as much as 30%, there is a potential to save thousands of lives.Various international aid agencies are investing in road infrastructure development.These initiatives could be coupled with the need to develop concrete epidemiological data.Innovative strategies, such as step wedge study design, 5 could lead to the evolution of an evidence based public health approach to this problem.
A courageous plan
Authors: M Turner, P McCrory
Journal: British Journal of Sports Medicine (2006)

How can the general population be encouraged to exercise enough to improve overall health?

In the corridors of Whitehall and similar government institutions world wide, new ideas are often viewed with suspicion. Consider this quote from the legendary TV series Yes, Prime Minister (Series 2, Episode 7: “The National Education Service”, BBC1, 21 Jan 1988) when Sir Humphrey Applebee responds to an idea from his Minister by suggesting the formation of a committee to consider a proposal:

Sir Humphrey: “I mean they’ll give it the most serious and earnest consideration and insist on a thorough and rigorous examination of all the proposals, allied with detailed feasibility study and budget analysis, before producing a consultative document for consideration by all interested bodies and seeking comments and recommendations to be included in a brief, for a series of working parties who will produce individual studies which will provide the background for a more wide ranging document, considering whether or not the proposal should be taken forward to the next stage.”Jim Hacker: “You mean they’ll block it?”Sir Humphrey: “ Yeah .” 

This sums up the feeling of many sports physicians who, armed with the knowledge that exercise is good for you, find the pathways to achieving government action blocked by interminable red tape. How then can we convince our political lords and masters to move forward on these ideas?

First, make it obvious.

The case for increasing exercise at a population level should be crystal clear even to a politician. Obesity has increased to the point where nearly two thirds of men and over …
Recording the Electrocardiogram during the Performance of the Master Two-Step Test: I
Authors: ISADORE ROSENFELD, ARTHUR M. MASTER, CAMILLA ROSENFELD
Journal: Circulation (1964)

Various technics for recording the electrocardiogram while the patient was exercising on the Master two-step apparatus were evaluated. Satisfactory tracings were obtained both by radiotelemetry (when the apparatus was properly aligned) and a direct hook-up technic that requires only standard equipment. Recommended electrodes are described, electrode positions discussed, and possible pitfalls of the technics enumerated. It is concluded that for the specific purpose of monitoring the two-step test, radiotelemetry is unnecessary and perhaps less desirable than direct hook-up monitoring.
Testosterone in Dwarfism
Authors: R. M. Acheson
Journal: BMJ (1957)

<h3>Background</h3> There is uncertainty regarding the predictors of early neurological deterioration (END) after endovascular thrombectomy in patients with acute ischemic stroke (AIS). Limited studies have focused on the effect of END on functional outcome. Our aim was to determine the predictors of END after endovascular thrombectomy in AIS and its effect on functional outcome at 90 days. <h3>Methods</h3> This is a secondary analysis of the DIRECT-MT trial. Patients who failed to complete endovascular thrombectomy were additionally excluded. END was defined as ≥4-point increase in National Institutes of Health Stroke Scale score between admission and 24 hours after endovascular thrombectomy. Multivariable logistic regression was used to identify predictors for END and its effect on the modified Rankin Scale (mRS) score at 90 days. <h3>Results</h3> Of 591 patients enrolled, 111 (18.8%) had postoperative END, which was associated with higher ordinal mRS score at 90 days (adjusted common OR (aOR) 6.968, 95% CI 4.444 to 10.926). Non-modifiable factors included baseline Alberta Stroke Program Early CT Score (aOR 0.883, 95% CI 0.790 to 0.987), systolic blood pressure (aOR 1.017, 95% CI 1.006 to 1.028), glucose level (aOR 1.178, 95% CI 1.090 to 1.273), collateral status (aOR 0.238, 95% CI 0.093 to 0.608), occlusion site (aOR 0.496, 95% CI 0.290 to 0.851) and the presence of an anterior communicating artery (aOR 0.323, 95% CI 0.148 to 0.707). Admission-to-groin puncture time (aOR 1.010, 95% CI 1.003 to 1.017), general anesthesia (aOR 2.299, 95% CI 1.193 to 4.444), number of passes (aOR 1.561, 95% CI 1.243 to 1.961) and contrast extravasation (aOR 6.096, 95% CI 1.543 to 24.088) were modifiable predictors for END. <h3>Conclusions</h3> Postoperative END is associated with adverse functional outcome. Several non-modifiable and modifiable factors can predict END and support future treatment decision-making to improve the potential utility of endovascular thrombectomy. <h3>Trial registration number</h3> DIRECT-MT ClinicalTrials.gov NCT03469206.
422. Dominant-Negative Interference in the Pahenu2 Mouse Model of PKU: Effectiveness of Vectors Expressing Either Modified Forms of Phenylalanine Hydroxylase (PAH) or Ribozymes Plus a Hardened PAH mRNA
Authors: Catherine E. Charron, O.P. Perera, Stacy L. Porvasnik, Mandy Blackburn, Alfred S. Lewin, Philip J. Laipis
Journal: Molecular Therapy (2005)

The common human genetic disorder Phenylketonuria (PKU) is primarily caused by defects in the enzyme phenylalanine hydroxylase (PAH). An animal model for PKU, the BTBR Pahenu2 mouse, has a missense mutation (F263S) that inactivates PAH; the mouse exhibits classic PKU, with elevated blood Phe levels, cognitive deficiencies, and maternal PKU syndrome. Like the Pahenu2 mouse, a majority of human PKU patients also have missense mutations. We have corrected both the serum Phe levels and maternal PKU in Pahenu2 mice using recombinant AAV vectors containing the mouse PAH gene. Although successful, unusually high vector doses were needed to normalize serum Phe levels. More critically, there was a gradual loss of therapeutic effect 20|[ndash]|24 weeks after vector delivery in many animals, particularly females.