tbuckley/cabot-search huggingface dataset:
983. Repeat Intra-Articular Administration of AAV2 Vectors to Rat Joints
Authors: Haim Burstein, Kurt Lustig, Ziv Sandalon
Journal: Molecular Therapy (2006)

Rheumatoid arthritis is a chronic autoimmune disease characterized by synovial inflammation, leading to the destruction of cartilage and bone. Previously, we demonstrated that a single intra-articular administration of AAV2-TNFR:Fc vector to rats with experimental arthritis resulted in suppression of disease. For treatment of chronic inflammatory arthritis using intra-articular delivery of AAV2- TNFR:Fc vector, intra-articular re-administrations may be required. In this study, we evaluated if serum anti-AAV2 capsid neutralizing antibodies, elicited after intra-articular administration of AAV2- TNFR:Fc vector to rat joints, affect joint transduction following intra-articular re-administration of an AAV2-FlagLuc vector. AAV2- FlagLuc was administered approximately one, three, nine and twelve months after injection of either vehicle or AAV2-TNFR:Fc vector. Two weeks post intra-articular injection of the AAV2-FlagLuc vector, we measured and compared luciferase enzyme activity in joint tissues of vehicle-treated animals with animals treated first with AAV2- ratTNFR:Fc vector. Intra-articular administration of AAV2-TNFR:Fc vector to rat joints elicited serum anti-AAV2 capsid neutralizing antibody response. Following re-administration with AAV2-FlagLuc, the anti- AAV2 capsid neutralizing antibody levels in the serum were up to 10-fold higher compared with those detected prior to vector re- administration. Luciferase enzyme activity, measured following re- administration of AAV2-FlagLuc vector to vehicle-treated animals, was 25-fold higher compared with the assay background in normal joints, and 12-fold higher in inflamed joints. In contrast, luciferase enzyme activity was not detected in normal or arthritic rat joints that were injected first with AAV2-ratTNFR:Fc and then re- administered with AAV2-FlagLuc. However, using RT-PCR analyses, we observed sustained expression of TNFR:Fc for at least 378 days. These results demonstrate that in rats the levels of serum anti- AAV2 capsid neutralizing antibodies, elicited one to twelve months after AAV2 vector administration to the rat joint, are sufficiently high to inhibit transduction following intra-articular re-administration with a vector of the same serotype. The evidence that TNFR:Fc is expressed in the joints for more than a year suggests that frequent repeat administrations to the joints may not be needed.
Discrimination between Crigler-Najjar type I and II by expression of mutant bilirubin uridine diphosphate-glucuronosyltransferase.
Authors: J Seppen, P J Bosma, B G Goldhoorn, C T Bakker, J R Chowdhury, N R Chowdhury, P L Jansen, R P Oude Elferink
Journal: Journal of Clinical Investigation (1994)

Crigler-Najjar (CN) disease is classified into two subtypes, type I and II. The molecular basis for the difference between these types is not well understood. Several mutations in the bilirubin UDP-glucuronosyl-transferase (B-UGT) gene of six CN type I and two CN type II patients were identified. Recombinant cDNAs containing these mutations were expressed in COS cells. B-UGT activity was measured using HPLC and the amount of expressed protein was quantitated using a sandwich ELISA. This enabled us to determine the specific activities of the expressed enzymes. All type I patients examined had mutations in the B-UGT1 gene that lead to completely inactive enzymes. The mutations in the B-UGT1 gene of patients with CN type II only partially inactivated the enzyme. At saturating concentrations of bilirubin (75 microM) CN type II patient A had 4.4 +/- 2% residual activity and CN type II patient B had 38 +/- 2% residual activity. Kinetic constants for the glucuronidation of bilirubin were determined. The affinities for bilirubin of B-UGT1 expressed in COS cells and B-UGT from human liver microsomes were similar with Km of 5.1 +/- 0.9 microM and 7.9 +/- 5.3 microM, respectively. B-UGT1 from patient B had a tenfold decreased affinity for bilirubin, Km = 56 +/- 23 microM. At physiological concentrations of bilirubin both type II patients will have a strongly reduced conjugation capacity, whereas type I patients have no B-UGT activity. We conclude that CN type I is caused by a complete absence of functional B-UGT and that in CN type II B-UGT activity is reduced.
Neuroendocrine responses to glucose ingestion in man. Specificity, temporal relationships, and quantitative aspects
Authors: Thomas F. Tse, William E. Clutter, Suresh D. Shah, J. Philip Miller, Philip E. Cryer
Journal: Journal of Clinical Investigation (1983)

The mechanisms of postprandial glucose counterregulation—those that blunt late decrements in plasma glucose, prevent hypoglycemia, and restore euglycemia—have not been fully defined. To begin to clarify these mechanisms, we measured neuroendocrine and metabolic responses to the ingestion of glucose (75 g), xylose (62.5 g), mannitol (20 g), and water in ten normal human subjects to determine for each response the magnitude, temporal relationships, and specificity for glucose ingestion. Measurements were made at 10-min intervals over 5 h. By multivariate analysis of variance, the plasma glucose (P < 0.0001), insulin (P < 0.0001), glucagon (P < 0.03), epinephrine (P < 0.0004), and growth hormone (P < 0.01) curves, as well as the blood lactate (P < 0.0001), glycerol (P < 0.001), and β-hydroxybutyrate (P < 0.0001) curves following glucose ingestion differed significantly from those following water ingestion. However, the growth hormone curves did not differ after correction for differences at base line. In contrast, the plasma norepinephrine (P < 0.31) and cortisol (P < 0.24) curves were similar after ingestion of all four test solutions, although early and sustained increments in norepinephrine occurred after all four test solutions. Thus, among the potentially important glucose regulatory factors, only transient increments in insulin, transient decrements in glucagon, and late increments in epinephrine are specific for glucose ingestion. They do not follow ingestion of water, xylose, or mannitol.
Detection of hepatitis C virus RNA in paraffin‐embedded tissues from patients with non‐Hodgkin's lymphoma
Authors: Semra Paydas, Melek Ergin, Kahraman Tanriverdi, Sinan Yavuz, Umut Disel, Nil Banu Kilic, Seyda Erdogan, Berksoy Sahin, İlhan Tuncer, Refik Burgut
Journal: American Journal of Hematology (2004)

Abstract The aim of this study is to detect the possible role of hepatitis C Virus (HCV) in lymphomagenesis. HCV‐RNA and anti‐HCV antibodies were studied in tissue and serum samples taken from patients with non‐Hodgkin's Lymphoma (NHL). The prevalence of HCV, the clinical presentation of these cases, and association with histologic subtypes were determined. RT‐PCR was used to detect the HCV‐RNA in serum and tissue samples. The anti‐HCV antibodies were tested with microparticle enzyme immunoassay. Immunohistochemistry with the ABC method was used to detect the HCV core protein in HCV‐RNA + cases. RNA could be detected in 30 of 35 cases, and other tests were performed in these 30 samples. HCV‐RNA was detected in 11 tissue samples (11/30, 37%). HCV core protein was studied in 10 of 11 HCV‐RNA + cases, and 1–3% nuclear staining was found in only 2 samples. Serologically, HCV‐RNA was detected in 7 of 30 samples (23.3%) and anti‐HCV antibody was detected in 3 of 30 samples (10%). Detection of HCV‐RNA in 37% of the lymphoma tissue samples suggests that HCV may have a role or is a contributing factor in the pathogenesis of lymphoma. The very low HCV core protein in lymphoma tissues may be due to the low viral load in lymphoid tissues and/or higher sensitivity of the PCR method. Detection of anti‐HCV antibody in only three cases may be associated with undetectable levels of antibodies due to the immune deficiency in cases with NHL. Am. J. Hematol. 76:252–257, 2004. © 2004 Wiley‐Liss, Inc.
Immunotherapy with cat- and dog-dander extracts II. In vivo and in vitro immunologic effects observed in a 1-year double-blind placebo study
Authors: G HEDLIN, V GRAFFLONNEVIG, H HEILBORN, G LILJA, K NORRLIND, K PEGELOW, B SUNDIN, H LOWENSTEIN
Journal: Journal of Allergy and Clinical Immunology (1986)
PSYCHIC PAIN AND THE PSYCHOSES
Authors: H. Devine
Journal: BMJ (1931)

Warthin" has discussed this factor and speaks of it as the Graves' constitution, but at present it must be regardvd as theoretical, because it cannot yet be recognized in the absence of the disease.It appears as if this factor is strong in some patients so that no other factor is needed to produce the disease, but that in other patients it is less strong, and in them some additional factors are necessary.The prognosis will be influenced by these considerations, and the restoration to health, even after thyroidectomy, will be less complete the stronger the constitutional factor.In some of the cases in which the result is recorded as " poor," the patients gave histories of nervousness and excitability all their lives, and appeared to have been below the normal standard of physical efficiency before the onset of symptoms of thyroid intoxication.This was clearly a factor in the results in the two following cases: Mlrs. A. B., aged 55 years.Nervous and excitable all her life.In 1923, aged 47 years, developed exophthalmic goitre; symptoms more severe following tonsillitis in I922.January, 1923, tonsillectomy; continued to be nervous and excitable, and unable to do her wvork.April, 1928, B.M.R.+20 per cent., very emotional and mentally depressed, and these synmptoms were more prominent than those of thyroid intoxication, which were mild.April, 1928, partial thyroidectomy.I931, im- proved, no evidence of thyroid intoxication, but always grumbling and unhappy, and not able to do her house-work.Miss F. B., aged 46 years.Never strong, always tired, numerous fainting attacks, frequent tonsillitis.In 1905, aged 20 years, developed exophthalmic goitre; remissions and re- lapses frequent, and very emotional. 1926-1929,repeated attacks of tetany caused by emotion, but no disturbance of calcium metabolism, no alkalosis.July,
The Minnesota living with heart failure questionnaire correlates with disability and clinical parameters in elderly patients with heart failure
Authors: G. Pulignano, D. Del Sindaco, A.P. Maggioni, D. Lucci, D. Fortuna‐Grillo, S. Maletti, I. Tuveri, R. Melecchi, C. Martinelli, C. Opasich
Journal: European Journal of Heart Failure (2000)
New year, new health?
Authors: W. Moore
Journal: BMJ (2011)

Whether it’s quitting smoking or forsaking whoring, eschewing snuff or joining the gym, more resolutions have probably been made—and broken—in pursuit of health than any other cause.

When the Georgian rake James Boswell arrived in London in 1762 he firmly resolved to “have nothing to do with whores,” to avoid a repeat of “the loathsome distemper” after first contracting gonorrhoea in his teens. But willpower was not his strong point. After a grand tour of casual sex throughout Europe he had 18 more visits from his old friend “Signor Gonorrhoea” before he died at 54 from the complications of venereal disease.

More successful was the 16th century …
Relationship of selected kinematics variables with the performance of back court spike in volleyball
Authors: Harish Kumar Tiwari, None Jaiprakash, None Amar
Journal: British Journal of Sports Medicine (2010)

The purpose of this study was to examine the relationship of selected kinematic variables with the performance of back court spike in volleyball. The subjects (5 M, age 20–24 years) participated in All India Inter University Volleyball. Pearson product moment correlation was used to analyse the linear (height of centre of gravity (CG) of the body and ball) and angular kinematic (angles of shoulder, elbow, hip, knee and ankle joints) variables at the moment of ball contact. The results have shown that the values of the co-efficient of correlation are at right ankle joint (−0.843), left ankle joint (−0.651), right knee joint (0.879), left knee joint (−0.593), right hip joint (−0.298), left hip joint (−0.081), right elbow joint (0.884), left elbow joint (−0.720), right shoulder joint (−0.560), left shoulder joint (−0.815), height of CG of body (0.112) and height of CG of ball (0.497) whereas tabulated value of 3 degree of freedom at 0.05 level of significance is 0.87. Value of coefficient of correlation in case of the angle at right elbow and right knee joint has shown the significant relationship whereas other selected biomechanical variables show an insignificant relationship at the 0.05 level of significance.
Are we providing doctors with the training and tools for lifelong learning?
Authors: A. F Shaughnessy, D. C Slawson
Journal: BMJ (1999)

<h3>Abstract</h3> Visuomotor feedback responses vary in intensity throughout a reach, commonly explained by optimal control. Here, we show that the optimal control for a range of movements with the same goal can be simplified to a time-to-target dependent control scheme. We measure our human participants’ visuomotor responses in five reaching conditions, each with different hand or cursor kinematics. Participants only produced different feedback responses when these kinematic changes resulted in different times-to-target. We complement our experimental data with a range of finite and non-finite horizon optimal feedback control (OFC) models, finding that the model with time-to-target as one of the input parameters best replicates the experimental data. Overall, this suggests that time-to-target is a critical control parameter in online feedback control. Moreover, we propose that for a specific task and known dynamics, humans can instantly produce a control signal without any additional online computation allowing rapid response onset and close to optimal control.
U.S. Health Care and the Future Supply of Physicians
Authors: B. S. Bloom
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Cardioversion and Digitalis
Authors: STEPHEN M. WITTENBERG, BERNARD LOWN
Journal: Circulation (1969)

Experiments were performed in anesthetized dogs to study some effects of propranolol, dextro-propranolol and ICI50,172 on digitalis-induced arrhythmias and digitalis-induced sensitization to electrical shock. When given in beta-blocking doses, propranolol did not abbreviate ouabain-induced ventricular tachycardia (VT) or decrease ouabain-induced sensitization to electrical shock. In larger doses, propranolol first controlled the arrhythmia without affecting shock sensitivity and then abolished both arrhythmia and shock sensitivity. Dextro-propranolol, a compound with nonspecific antiarrhythmic action equal to that of propranolol but with virtually no beta-blocking effect, abolished shock sensitivity, whereas ICI50,172, a pure beta-blocking agent, did not. Beta-blocking doses of propranolol given to dogs bordering on digitalis toxicity but in normal sinus rhythm caused recurrence of sustained ventricular tachycardia. Reappearance of toxicity appeared to be related to a propranolol-induced decrease in heart rate, since it was averted during continuous electrical pacing of the atrium and reversed by isoproterenol administration.
373 THE EVALUATION OF EFFECTIVENESS AND SAFETY FOR NEW THERAPY WITH BONE MARROW DERIVED AUTOLOGOUS MESENCHYMAL STEM CELL FOR HEPATIC FAILURE CAUSED BY ALCOHOLIC LIVER CIRRHOSIS; PHASE II STUDY
Authors: M.Y. Kim, S.K. Baik, S.Y. Park, J.H. Hong, S.O. Kwon, G.J. Cheon, Y.D. Kim, Y.-O. Jang, H.K. Hwang, H.A. Kim, H.W. Kang, J.M. Jo
Journal: Journal of Hepatology (2012)
Bone Dysplasias: An Atlas of Constitutional Disorders of Skeletal Development
Authors: Jonathan Cohen
Journal: JAMA (1975)

The dysplasias of bone collectively affect a large number of individuals, but each dysplasia, if not rare, is certainly uncommon. This book lists 82 separate diseases and illustrates each one. For each entity, a one-page characterization is included along with a bibliography of a few articles. The authors are such acknowledged experts in diagnosis in this restricted field that one may be tempted to ask too much of their book. For instance, one would look in vain for a roentgenographic classification of dysplasias that is logical or more useful than any other previously proposed. Their radiological classification groups the syndromes according to eight criteria: skeletal dysplasias with predominantly epiphyseal involvement; skeletal dysplasias with predominantly metaphyseal involvement; skeletal development with major involvement of the spine; mucopolysaccharidoses and mucolipidoses; skeletal dysplasias due to anarchic development of bone constituents; osteolyses; skeletal dysplasias with predominant involvement of single sites or segments; and skeletal dysplasias
Unilateral hyperhidrosis associated with underlying intrathoracic neoplasia.
Authors: D C Lindsay, J G Freeman, C O Record
Journal: Thorax (1986)

Intrathoracic neoplasia is notable for the many ways in which it may present.We would like to report two cases demonstrating a rare association between unilateral local- ised hyperhidrosis of the thoracic cage and underlying intra- thoracic neoplasm. Case reports CASE 1A 67 year old retired shotblaster complained of a 3 kg weight loss, mild dyspnoea, chest pain localised to the right costal margin, and profuse sweating localised to an area below the right scapula.He smoked 15 cigarettes per day.Examination confirmed a right sided localised band of sweating at the level of T6-9 posteriorly.Apart from minimal winging of the right scapula and some wasting of the right suprascapular muscles no abnormal neurological signs were detected.A chest radiograph showed a large right pleural effusion with collapse of the underlying lung.Analysis of the pleural fluid revealed no malignant cells or acid fast bacilli, but a pleural biopsy confirmed the presence of poorly differentiated car- cinoma.A technetium radioisotope bone scan showed increased uptake in the anterior ends of the right 4th and 5th ribs.The abnormal sweating continued for a further six months but the patient's condition deteriorated with the development of intra-abdominal metastases and recurrent pleural effusions.He died at home; necropsy was not per- formed .CASE 2
Abstract 1290: Survival in familial breast cancer: Novel findings based on a nationwide prospective cohort study
Authors: Elham Kharazmi, Asta Försti, Kristina Sundquist, Kari Hemminki
Journal: Cancer Research (2014)

Abstract Objective: To find out whether breast cancer (BC) patients with positive family history of concordant cancer are diagnosed at earlier TNM stages and to explore the survival in familial breast cancer compared to sporadic cases who were diagnosed at identical TNM status and staging. Design: Nationwide prospective cohort study. Setting and method: The nationwide Swedish Family-Cancer Database including all Swedes born after 1931 and their biological parents, totaling &amp;gt;14.7 million individuals. Hazard ratios (HR) were calculated for 4,709 women with BC in a first-degree relative (FDR) versus 25,366 BC women without such a family history. Results: Familial BC cases were not diagnosed at earlier stages compared to sporadic cases. There was no difference in survival of familial BC patients who were diagnosed at high TNM staging (T2-T4, N1-N3, M1) compared to sporadic cases diagnosed at the same late stage. Young BC patients (age&amp;lt;40) in early stages (T0-T1, N0, M0, Stage 0-I) had the worst survival when their FDR were diagnosed with single (HR: 2-3.7) or multiple (HR: 2.4-7.1) BC at any age. Conclusion: Young BC patients (age&amp;lt;40), diagnosed at early stages (T0-T1, N0, M0, stage 0-I), whose FDR were diagnosed with BC at any age had the higher risk of mortality compared to sporadic cases at the same stages. Our results offer novel information for personalized genetic counseling and treatment modalities and urge the need for identifying the underling genetic component for such a difference in survival of familial breast cancer. Citation Format: Elham Kharazmi, Asta Försti, Kristina Sundquist, Kari Hemminki. Survival in familial breast cancer: Novel findings based on a nationwide prospective cohort study. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1290. doi:10.1158/1538-7445.AM2014-1290
AUTOIMMUNE DISEASE IN NZB/BL MICE
Authors: Robert C. Mellors
Journal: The Journal of Experimental Medicine (1966)

The transplantation of spleen cells from old NZB/Bl mice with renal disease induced both the structural and the functional changes of membranous glomerulonephritis in young NZB/Bl mice within a few weeks and well in advance of its usual spontaneous occurrence. The development of hypergammaglobulinemia and lymphoid cell hyperplasia in the young mice indicated that immunologically competent cells, derived from either the transplant or the recipient, proliferated during this process. These experiments, together with other findings, provide further support for the view that membranous glomerulonephritis in NZB/Bl mice is produced by immunological, and probably autoimmune, mechanisms and that the renal disease is apparently almost wholly unrelated to the hemolytic process.