tbuckley/cabot-search huggingface dataset:
Mutation of the tumour suppressor <i>p33</i> <sup> <i>ING1</i> <i>b</i> </sup> is rare in melanoma
Authors: M. Stark, J.A. Puig-Butille, G. Walker, C. Badenas, J. Malvehy, N. Hayward, S. Puig
Journal: British Journal of Dermatology (2006)

Background The p33ING1b gene is involved in the p53-dependent response to DNA damage following exposure to ultraviolet radiation, and has recently been reported to be mutated in 20% of melanoma tumours. Objectives We sought to assess the p33ING1b mutation rate in our large panels of fresh melanomas and melanoma cell lines. Methods We screened 83 primary melanomas and 55 melanoma cell lines for mutations in p33ING1b by single-strand conformational polymorphism analysis and by direct sequencing. Results In contrast to previous reports, we found no somatic p33ING1b mutations in our panel of melanomas. We found that some of the discrepancy between our results and previously published studies may be due to inadvertent amplification of the ING1 pseudogene (INGX), and/or contamination of some samples with murine Ing1. Conclusions p33 ING1b mutations in melanoma are rare. We have highlighted the importance of allele-specific primer design to avoid pseudogene amplification, and also the necessity to confirm the genetic identity and species of origin of individual cell lines. Further studies are needed to clarify the possible role of p33ING1b in melanoma tumorigenesis.
Acellular Vaccines and Resurgence of Pertussis
Authors: Eugene D. Shapiro
Journal: JAMA (2012)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Asbestos fibers, plasma and inflammation.
Authors: J A Hamilton
Journal: Environmental Health Perspectives (1983)

Fibrin clots have been detected at sites of inflammation, and kinins have been implicated as mediators of the vascular phenomena of acute inflammation, systemic shock, and disseminated intravascular coagulation. It is now reported that both negatively and positively charged asbestos fibers shorten the partial thromboplastin time of human plasma, indicating coagulation of the plasma. A sample containing short (less than 5 micron in length) chrysotile fibers is ineffective. Only the negatively charged amphiboles (crocidolite and amosite) are able to activate factor XII (Hageman factor). This particular effect of the amphiboles is enhanced by high molecular weight kininogen and leads to kinin formation.
Mice that lack the interferon-gamma receptor have profoundly altered responses to infection with Bacillus Calmette-Guérin and subsequent challenge with lipopolysaccharide.
Authors: R Kamijo, J Le, D Shapiro, E A Havell, S Huang, M Aguet, M Bosland, J Vilcek
Journal: The Journal of Experimental Medicine (1993)

Mice with a targeted disruption of the interferon gamma receptor gene (IFN-gamma R0/0 mice) and control wild-type mice were inoculated with the Bacillus Calmette-Guérin (BCG) strain of Mycobacterium bovis. BCG infection was not lethal for wild-type mice whereas all IFN-gamma R0/0 mice died approximately 7-9 wk after inoculation. Histological examination at 2 and 6 wk after BCG inoculation showed that livers of IFN-gamma R0/0 mice had higher numbers of acid-fast bacteria than wild-type mice, especially at 6 wk. In parallel, the livers of IFN-gamma R0/0 mice showed a reduction in the formation of characteristic granulomas at 2 wk after inoculation. Injection of lipopolysaccharide (LPS) 2 wk after BCG inoculation was significantly less lethal for IFN-gamma R0/0 mice than for wild-type mice. Reduced lethality of LPS correlated with a drastically reduced production of tumor necrosis factor alpha (TNF-alpha) in the IFN-gamma R0/0 mice. Interleukin 1 alpha (IL-1 alpha) and IL-6 levels in the serum were also significantly reduced in the IFN-gamma R0/0 mice after BCG infection and LPS challenge. The greatly reduced capacity of BCG-infected IFN-gamma R0/0 mice to produce TNF-alpha may be an important factor in their inability to resist BCG infection. These results show that the presence of a functional IFN-gamma receptor is essential for the recovery of mice from BCG infection, and that IFN-gamma is a key element in the complex process whereby BCG infection leads to the sensitization to endotoxin.
Depolarization-Transcription Signals in Skeletal Muscle Use Calcium Flux through L Channels, but Bypass the Sarcoplasmic Reticulum
Authors: Jakob Schmidt
Journal: Neuron (1998)

We have previously (C.-F. Huang, B. E. Flucher, M. M. Schmidt, S. K. Stroud, and J. Schmidt Neuron 13, 167–177 [1994].) reported data suggesting that in C2 cells, a mouse muscle cell line, calcium ions from the extracellular medium but not from intracellular stores inhibit activity of the genes coding for the subunits of the nicotinic acetylcholine receptor. However, we have not been able more recently, despite considerable effort, to reproduce the gene activity experiments described in the article and originally conducted by the first author, C.-F. Huang. In particular, we can no longer find evidence that C2 cells respond to membrane depolarization with a rapid inactivation of acetylcholine receptor genes. Consequently, whether and to what extent the sarcoplasmic reticulum is involved in signal transduction from the sarcolemma to these genes remain unknown. I apologize for any difficulities the publication of the unsubstantiated report may have caused.
Exercise-associated hyponatraemia after a marathon: case series
Authors: A M Goudie, D S Tunstall-Pedoe, M Kerins, J Terris
Journal: Journal of the Royal Society of Medicine (2006)

ObjectivesTo review the presentation, treatment and response of those runners from the London Marathon who presented to St Thomas' Hospital with exercise induced hyponatraemia. SettingSt Thomas' Hospital, a tertiary hospital situated near the finish line of the 2003 London Marathon. ParticipantsAll runners who presented to St Thomas' Hospital on the day of the 2003 London Marathon with altered mental state whose serum sodium concentration was less than 135 mmol/L. Main outcome measuresPresenting symptoms, volume and type of fluids administered and response to treatment (biochemical and clinical). ResultsFourteen patients were diagnosed with exercise associated hyponatraemia with serum sodium concentrations ranging from 116 to 133 mmol/L. Eleven presented with confusion. There were long delays between the finish time and presentation time for some runners. Anecdotal descriptions suggested some runners finished the race with normal mental state then became confused. There was no correlation between running time and serum sodium level. All patients received 0.9% saline and six received 1.8% saline. Despite this, some patients demonstrated falls in serum sodium concentrations. Thirteen to fourteen patients were symptomatically well the following morning, with the remaining patient significantly improved. ConclusionPresentation of exercise associated hyponatraemia may be delayed. Optimal treatment is controversial, but the use of isotonic saline may not result in rises of serum sodium and we would suggest the early use of hypertonic fluids in symptomatic patients.
Genetic Counseling for the Older Pregnant Woman: New Data and Questions
Authors: Lewis B. Holmes
Journal: New England Journal of Medicine (1978)

It has become accepted medical practice to inform pregnant women 35 years of age and older of their increased risk of having a child with chromosome abnormality in comparison to younger women, and to offer prenatal diagnosis to determine whether the fetus is affected. The focus has been on the older pregnant woman because the autosomal trisomies 21 (the Down syndrome), 18 and 13 have been found to be more common in the children of older mothers and because most of the affected infants have been born to women over 35.1 It has therefore been postulated that the extra chromosome . . .
Localization of the site of ventricular preexcitation with body surface maps in patients with Wolff-Parkinson-White syndrome.
Authors: D W Benson, R Sterba, J J Gallagher, A Walston, M S Spach
Journal: Circulation (1982)

Forty-nine patients with Wolff-Parkinson-White syndrome, ages 7 weeks to 51 years, were studied with isopotential body surface maps during normal sinus rhythm, atrial pacing or induced atrial fibrillation. The location of the accessory pathway was determined by multicather electrophysiologic study or surgical ablation of the accessory pathway. When fusion was minimized and ventricular activation primarily controlled by a single accessory pathway, the distribution of positive and negative potentials on the anterior and posterior torso during QRS (observed at 40 msec) and the ST segment were an excellent index of the location of the site of the accessory pathway. The relationship between a specific sequence of QRS-T wave body surface maps and a specific preexcitation site was similar from patient to patient in the presence of marked differences in age, size, and different cardiac status due to structural congenital cardiac defects. The localization of the site of the accessory pathway using distributions too early in QRS (before 40 msec) was unreliable because the early distributions varied from patient to patient for the same preexcitation site; however, the potential distributions during the ST segment were both stable and consistent from patient to patient for the same preexcitation site. The presence of significant fusion of ventricular activation initiated via a single accessory pathway and the normal conduction system or via multiple accessory pathways complicated the interpretation of body surface distributions. Thus, one can predict accurately at least seven preexcitation sites by the combined use of QRS and ST-segment body surface maps.
RUNX1 DNA-binding mutations and RUNX1-PRDM16 cryptic fusions in BCR-ABL+ leukemias are frequently associated with secondary trisomy 21 and may contribute to clonal evolution and imatinib resistance
Authors: Catherine Roche-Lestienne, Lauréline Deluche, Sélim Corm, Isabelle Tigaud, Sami Joha, Nathalie Philippe, Sandrine Geffroy, Jean-Luc Laï, Franck-Emmanuel Nicolini, Claude Preudhomme
Journal: Blood (2008)
Antibacterial antibody responses associated with the development of asthma in house dust mite-sensitised and non-sensitised children
Authors: Belinda J Hales, Lee Ying Chai, Claire E Elliot, Leigh J Pearce, Guicheng Zhang, Tatjana K Heinrich, Wendy-Anne Smith, Merci M Kusel, Patrick G Holt, Peter D Sly, Wayne R Thomas
Journal: Thorax (2011)

<h3>Background</h3> Infants who develop house dust mite (HDM) allergy and HDM-sensitised children with severe persistent asthma have low antibody responses to the P6 antigen of <i>Haemophilus influenzae</i>. <h3>Objective</h3> To measure the development of antibody to two ubiquitous bacteria of the respiratory mucosa in a prospective birth cohort at high risk of allergic disease and to assess which responses are associated with asthma and atopy. <h3>Methods</h3> IgG1 and IgG4 antibody to <i>H influenzae</i> (P4 and P6) and <i>Streptoccocus pneumoniae</i> (PspA and PspC) surface antigens was measured in yearly blood samples of children aged 1–5 years. IgE to the P6 antigen was examined for the 5-year group. The children were stratified based on HDM sensitisation and asthma at 5 years of age. <h3>Results</h3> HDM-sensitised children had lower IgG1 antibody titres to the bacterial antigens, and early responses (&lt;3 years and before the development of HDM sensitisation and asthma) corrected for multiple antigens were significantly reduced for P4, P6 and PspC (p=0.008, p=0.004 and p=0.028, respectively). Similar associations with asthma were also found (p=0.008, p=0.004 and p=0.032 for P4, P6 and PspC, respectively). The IgG4 antibody titre and prevalence were similar in both HDM-sensitised and non-sensitised groups, but sensitised children had a slower downregulation of the IgG4 response. Children with asthma (27/145 at 5 years) had lower anti-P6 IgE responses (p&lt;0.05). <h3>Conclusions</h3> HDM-sensitised children have early defective antibody responses to bacteria that are associated with asthma. Surprisingly, antibacterial IgE was associated with a reduced risk for asthma.
Queer Science: The Use and Abuse of Research on Homosexuality
Authors: Domeena C. Renshaw
Journal: JAMA (1996)

The author of<i>Queer Science</i>, until recently a neuroscientist at the Salk Institute in San Diego, Calif, researched and in 1991 published in<i>Science</i>his admittedly inconclusive report. In a small postmortem study of the brains of 13 homosexual men with AIDS, he found the interstitial nucleus of the anterior hypothalamus (INAH) was smaller in some than the INAH of comparable heterosexual males. Although limiting factors were the small sample size, the diagnosis of AIDS, and medications used, the as yet unreplicated findings did stir up renewed interest and longstanding academic, social, personal, philosophical, moral, media, and legal questions about a possible anatomic cause of homosexual orientation (gender identity and feelings). The 295-page text has 50 additional pages of references and notes. It attempts to gather the research objectively, while the message comes through strongly: gays are born that way. The title's "Queer" may perhaps be trendy, cute, or tongue-in-cheek
Functional renal failure and haemorrhagic gastritis associated with endotoxaemia in cirrhosis.
Authors: C Clemente, J Bosch, J Rodes, V Arroyo, A Mas, S Maragall
Journal: Gut (1977)

Forty-three patients with cirrhosis and ascites, 21 with normal renal function, 10 with a progressive functional renal failure (FRF), and 12 with a steady FRF, were investigated for the presence of endotoxaemia by the Limulus lysate test. Endotoxaemia was found in nine patients with FRF and in none of the 21 with normal renal function (P less than 0-01). A positive Limulus test was almost exclusively associated with a progressive FRF (eight of 10 patients) and all but one of them died. Renal function improved as endotoxaemia disappeared in the survivor. Endotoxaemia was also associated with haemorrhage due to acute erosions of the gastric mucosa, being present in six of the seven patients who had this complication. Intravascular coagulation was not found in any patient. The Limulus test was positive in the ascitic fluid in 18 of 21 patients tested, although only two of them had peritonitis. These results suggest that endotoxaemia may play a critical role in the development of progressive renal failure and haemorrhagic gastritis in cirrhosis, and emphasise the potential risk of procedures involving reinfusion of ascitic fluid.
GASTRIC ULCER AND GASTRIC CANCER
Authors: W. Gordon
Journal: BMJ (1921)

Have a career or related problem that needs answering? Can9t find the right person to point you in the right direction? Log on to the Advice Zone (www.bmjcareers.com/advicezone) for reliable medical careers advice. You can post a question or see if one of our 300 advisers has already answered a similar question. Here is a selection of questions and answers posted on the site.
Ahead of the game
Authors: Rebecca Coombes
Journal: BMJ (2008)

Private hospital group Bupa, now owned by Spire Healthcare, has been collecting patient reported outcome (PROMS) data for the past decade. In this sense, they are ahead of the NHS, which has recently agreed to collect data in four areas: hip replacement, knee replacement, inguinal hernia, and varicose veins.

The initial motivation was to make systems more robust in the wake of the serious malpractice of NHS consultant gynaecologist Rodney Ledward, who also treated patients in the private sector. Andrew Vallance-Owen, Bupa group medical director, explains: “I went through [Ledward’s] details and there were no deaths, no readmissions, no complaints—there was nothing to tell us about him. …
Cesarean Delivery for Women Presenting With Genital Herpes Lesions
Authors: Adrienne G. Randolph
Journal: JAMA (1993)

<h3>Objective.</h3> —To assess the effect of cesarean delivery on neonatal and maternal morbidity and mortality and their associated costs for two populations of women presenting with genital herpes lesions at delivery: those with and those without a history of genital herpes. <h3>Data Sources.</h3> —MEDLINE (search for herpes simplex virus and neonatal, cesarean, and mortality) and recognized experts. <h3>Data Extraction.</h3> —The quality of the overall data used for baseline values was graded using a predetermined scale. <h3>Results.</h3> —The practice of cesarean delivery for women with a history of genital herpes lesions that recur at delivery results in more than 1580 excess cesarean deliveries performed for every poor neonatal outcome prevented, a cost per neonatal herpes case averted of $2.5 million and a cost per quality-adjusted life-year gained of $203 000. For these women, lowering the efficacy of cesarean delivery or the herpes simplex virus vertical transmission rate could result in maternal deaths outnumbering neonatal deaths prevented. In contrast, cesarean delivery for women with no history of genital herpes simplex virus who have lesions at delivery has low maternal costs per neonatal benefit and saves money. <h3>Conclusions.</h3> —Women who present with their first clinical episode of genital herpes at delivery should have a cesarean section performed. However, the current practice of cesarean delivery for women with a history of genital herpes lesions that recur at delivery results in high maternal morbidity and mortality at substantial financial expense, underscoring the urgency of examining alternative management strategies. (<i>JAMA</i>. 1993;270:77-82)
Psychodynamic Aspects in Young Patients Suffering from Peripheral Vascular Occlusions
Authors: K. Köhle, C. Simons
Journal: Psychotherapy and Psychosomatics (1970)

We found in the patients suffering from peripheral vascular occlusions typical psychodynamic processes, similar action- and reaction-patterns, a characteristic parental constellation and according to drive- and defense-structure specific conflict situations that occurred before manifestation of the disease. We assume our results, which are similar to those concerning coronary patients as reported by other authors, to be specific for patients suffering from early manifestations of arteriosclerosis, and to allow the deduction and formulation of more specific questions.
Cardiology for the Primary Care Physician
Authors: Samuel Zoneraich
Journal: JAMA (1997)

Cardiology, Primary Care The restructuring of medical care requires the primary care physician to apply more efficient methods and judiciously refer the patient to the specialist and the hospital. Practitioners need solid, up-to-date knowledge in many medical specialties. Keeping the primary care physician well informed is not an easy task. To that end,<i>Cardiology for the Primary Care Physician</i>, edited by Alpert, fills a special role. The text's principal asset is its condensed, authoritative, and well-written chapters. Berg and Sebastian in their opening chapter on physical diagnosis demonstrate its central role in patient evaluation,<sup>1</sup>demonstrating the value of the older, noninvasive techniques in diagnosing heart disease. As the chapter is brief, one cannot expect a thorough discussion of all features of bedside cardiology, such as the pericardial rub, loudness of the first and second heart sounds, an apical heave detected by palpation pointing to left ventricular hypertrophy,<sup>2</sup>and
Helping Smokers Quit
Authors: Joan Stephenson
Journal: JAMA (1998)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Hospital investigation of men and women treated for angina
Authors: I. Spencer, N. Unwin, G. Pledger
Journal: BMJ (1995)

prevent the further diversion of scarce resources into pointless clerical tasks that have nothing to do with improving health care or the management of the NHS.I thank the extracontractual referrals manager for generous help with data collection.This work was carried out while I was a commissioning manager for acute services in a health authority.
The Editor, 'The British Journal of Dermatology'.
Authors: G. H. Findlay
Journal: British Journal of Dermatology (1965)

Journal Article The Editor, 'The British Journal of Dermatology'. Get access G. H. Findlay G. H. Findlay Pretoria Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 77, Issue 1, 1 January 1965, Pages 63–64, https://doi.org/10.1111/j.1365-2133.1965.tb14569.x Published: 01 January 1965