tbuckley/cabot-search huggingface dataset:
Adenovirus 5 Fibers Mutated at the Putative HSPG-binding Site Show Restricted Retargeting with Targeting Peptides in the HI Loop
Authors: Angelika B Kritz, Campbell G Nicol, Kate L Dishart, Ruth Nelson, Susan Holbeck, Dan J Von Seggern, Lorraine M Work, John H McVey, Stuart A Nicklin, Andrew H Baker
Journal: Molecular Therapy (2007)

Adenoviral vectors are commonly used for liver-directed gene therapy following systemic administration owing to their strong propensity for hepatocyte transduction. However, many disease applications would benefit from the delivery of adenoviruses to alternate tissues via this route. Research has thus focused on stripping the virus of native hepatic tropism in conjunction with modifying virus capsid proteins to incorporate novel tropism. Recently, the KO1S* adenovirus serotype 5 fiber mutant, devoid of both coxsackie and adenovirus receptor binding in the fiber knob domain and mutated at the putative heparan sulphate proteoglycan binding site in the fiber shaft, was shown to possess strikingly poor hepatic tropism in mice, rats, and non-human primates. Thus, it is an ideal candidate for retargeting strategies. We therefore assessed the ability of peptide-modified KO1S* fibers to retarget adenovirus. Peptide insertions were well tolerated and virions produced to high titers. However, expected retargeting at the level of transduction was not observed, despite cell-binding studies showing enhanced vector targeting at the cell surface. Cy3 labeling studies showed retarded trafficking of S*-containing fibers. Taken together, our data demonstrates that KO1S* mutant fibers are ineffective for cell retargeting strategies. Adenoviral vectors are commonly used for liver-directed gene therapy following systemic administration owing to their strong propensity for hepatocyte transduction. However, many disease applications would benefit from the delivery of adenoviruses to alternate tissues via this route. Research has thus focused on stripping the virus of native hepatic tropism in conjunction with modifying virus capsid proteins to incorporate novel tropism. Recently, the KO1S* adenovirus serotype 5 fiber mutant, devoid of both coxsackie and adenovirus receptor binding in the fiber knob domain and mutated at the putative heparan sulphate proteoglycan binding site in the fiber shaft, was shown to possess strikingly poor hepatic tropism in mice, rats, and non-human primates. Thus, it is an ideal candidate for retargeting strategies. We therefore assessed the ability of peptide-modified KO1S* fibers to retarget adenovirus. Peptide insertions were well tolerated and virions produced to high titers. However, expected retargeting at the level of transduction was not observed, despite cell-binding studies showing enhanced vector targeting at the cell surface. Cy3 labeling studies showed retarded trafficking of S*-containing fibers. Taken together, our data demonstrates that KO1S* mutant fibers are ineffective for cell retargeting strategies.
Radiation, not enucleation, for choroidal melanoma?
Authors: Milan Korcok
Journal: JAMA (1983)

An iridium 192 wire mold that can bracket and irradiate a large malignant melanoma of the choroid may provide an alternative to enucleation of the affected eye. The mold, discussed at the recent annual meeting of the American Society of Therapeutic Radiologists in Orlando, Fla, is being used experimentally at the Princess Margaret Hospital in Toronto. Its present application is in patients whose tumors are too large or inappropriately located to be treated with external beam radiation or cobalt 60 plaques. Generally, large tumors anterior to the equator of the eye are hard to treat effectively with more conventional radiation sources because external radiation from an anterior aspect may damage the lens itself. Clinical physicist Barbara Japp, BSc, told the meeting that the wire mold—surgically implanted over the base of the tumor (which usually is growing pyramid-like into the eye) and left in place for five to seven days—can be
A direct radioimmunoassay for human renin substrate and identification of multiple substrate types in plasma.
Authors: P Eggena, J D Barrett, H Hidaka, C L Chu, C Thananopavarn, M S Golub, M P Sambhi
Journal: Circulation Research (1977)

Plasma renin substrate, a widely measured parameter of the renin reaction, is quantitated indirectly by the measurement of liberated angiotensin I upon exhaustive incubation of plasma with added renin. To overcome methodological problems of this assay system, we have developed a direct radioimmunoassay for this plasma protein using renin substrate purified from pooled plasma of normotensive subjects as the antigen. Comparison of substrate quantitated by the two assay systems (direct and indirect) indicates a 1:1 correlation with the exception of certain subjects with elevated substrate levels induced by estrogen therapy. To study the possibility of multiple substrate forms, we have made a comparison of substrate quantitated by both radioimmunoassays in conjunction with electrophoresis of plasma on polyacrylamide gel. One major form of substrate with a retardation factor (Rf) = 0.60 was found in normotensive and essential hypertensive subjects which gave a 1:1 correspondence on quantitation by the two methods. In contrast, six of 16 women on oral contraceptives demonstrated three forms of substrate (Rf = 0.16, 0.35, and 0.60) on electrophoresis. Substrate with Rf = 0.16 and 0.35 did not cross-react with the antiserum prepared against substrate from normotensive subjects, implying structural differences in these proteins.
Gallbladder Disease and Angina Pectoris
Authors: Arthur B. French
Journal: Annals of Internal Medicine (1992)

Letters15 July 1992Gallbladder Disease and Angina PectorisArthur B. French, MDArthur B. French, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-117-2-172_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the Editors: The article by Ryan and colleagues (1) calls to mind the occasional occurrence of high substernal chest pain, clearly related to exercise and relieved promptly by nitroglycerine in patients who also have gallstones. In some patients, removal of the gallstones is followed by disappearance of the angina. The late Dr. Chester Jones at Massachusetts General Hospital, who may have been the first to note this phenomenon, proposed that an overflow of autonomic nervous stimuli from the gallbladder raised the level of autonomic activity in the coronary vessels. Consequently, a smaller exercise stimulus would be needed to exceed...Reference1. Ryan E, Pak P, and DeSanctis R. Myocardial infarction mimicked by acute cholecystitis. Ann Intern Med. 1992;116:218-20. LinkGoogle Scholar1. Miller C. The gall-bladder and cardiac pain. Lancet. 1932;1:767-72. CrossrefGoogle Scholar2. Ryan E, Pak P, and DeSanctis R. Myocardial infarction mimicked by acute cholecystitis. Ann Intern Med. 1992;116:218-20. LinkGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: Arthur B. French, MD PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byGastrocardiac Afferent Convergence in Upper Thoracic Spinal Neurons: A Central Mechanism of Postprandial Angina Pectoris 15 July 1992Volume 117, Issue 2Page: 172-172KeywordsAnginaExerciseGallbladderIschemia ePublished: 1 December 2008 Issue Published: 15 July 1992 PDF downloadLoading ...
COLORADO TICK FEVER AND DENGUE
Authors: Lloyd Florio, William McD. Hammon, Angela Laurent, Mabel O. Stewart
Journal: The Journal of Experimental Medicine (1946)

Six human beings were inoculated with dengue and developed typical disease. Two of these were reinoculated and proved immune. The remaining four were later inoculated with Colorado tick fever. Three developed typical disease. The fourth, who remained well, has previously lived in an endemic area (Colorado). One patient was inoculated with Colorado tick fever first and later with dengue. He developed both diseases. Colorado tick fever and dengue do not give a cross-immunity. Hamsters can be infected with Colorado tick fever but not with dengue. Colorado tick fever and dengue appear to be distinct disease entities.
Irradiation-Mediated Rescue of T Cell–Specific V(D)j Recombination and Thymocyte Differentiation in Severe Combined Immunodeficient Mice by Bone Marrow Cells
Authors: Chiyu Wang, Molly A. Bogue, Jonathan M. Levitt, David B. Roth
Journal: The Journal of Experimental Medicine (1999)

In SCID (severe combined immunodeficient) mice, proper assembly of immunoglobulin and T cell receptor (TCR) genes is blocked by defective V(D)J recombination so that B and T lymphocyte differentiation is arrested at an early precursor stage. Treating the mice with gamma irradiation rescues V(D)J rearrangement at multiple TCR loci, promotes limited thymocyte differentiation, and induces thymic lymphomas. These effects are not observed in the B cell lineage. Current models postulate that irradiation affects intrathymic T cell precursors. Surprisingly, we found that transfer of irradiated SCID bone marrow cells to unirradiated host animals rescues both TCR rearrangements and thymocyte differentiation. These data indicate that irradiation affects precursor cells at an earlier stage of differentiation than was previously thought and suggest new models for the mechanism of irradiation rescue.
Hypomagnesemia Causes Coronary Artery Spasm
Authors: Manning Hanline
Journal: JAMA (1985)

<h3>To the Editor.—</h3> Low extracellular magnesium (Mg) levels may cause coronary artery spasm.<sup>1</sup>Increased influx of calcium across the arterial smooth-muscle cell membrane is the postulated mechanism.<sup>2</sup>Nevertheless, one authoritative review of calcium channel-blocking agents does not mention Mg.<sup>3</sup>Furthermore, I know of no case reports of hypomagnesemia causing cardiac ischemia. Therefore, the following brief report may be of interest. <h3>Report of a Case.—</h3> A 74-year-old woman was hospitalized because of syncope followed by chest pain. Past history included a myocardial infarction one month previously. At that time, her serum Mg level was 1.1 mg/dL (reference range, 1.4 to 2.0 mg/dL). Her chest pain had continued until she was given diltiazem, 30 mg orally three times daily. Coronary angiography one month before her myocardial infarction had shown no obstructive lesions, but ergonovine challenge was not performed. Twelve years prior to this, she had had a partial small-bowel
Esophageal Spasm and Psychiatric Disorder
Authors: Marvin M. Schuster
Journal: New England Journal of Medicine (1983)

The increasing complexity of scientific knowledge and investigative techniques has fostered a tendency for research to focus on smaller and smaller components because compartmentalization makes the task more manageable. Unfortunately, this trend has sometimes led to the view that the more finely dissected a study is, the more scientific it is. In lamenting the state of medical research in the 19th century, William James complained, many people nowadays seem to think that any conclusion must be very scientific if the arguments in favor of it are all derived from the twitching of frogs' legs — especially if the frogs are . . .
The spectranetics laser sheath reduces extraction time for nonthoracotomy defibrillator leads
Authors: M.J. Niebauer, A. Al-Khadra, M.K. Chung, G.A. Kidwell, V. Morant, H. Smith, P.J. Tchou, B.L. Wilkoff
Journal: Journal of the American College of Cardiology (1998)
ASXL1 and SETBP1 mutations and their prognostic contribution in chronic myelomonocytic leukemia: a two-center study of 466 patients
Authors: M M Patnaik, R Itzykson, T L Lasho, O Kosmider, C M Finke, C A Hanson, R A Knudson, R P Ketterling, A Tefferi, E Solary
Journal: Leukemia (2014)
Vasopressin and renin in glycerol-induced acute renal failure in the rat.
Authors: K G Hofbauer, A Konrads, K Bauereiss, B Möhring, J Möhring, F Gross
Journal: Circulation Research (1977)

Acute renal failure was induced in dehydrated rats by intramuscular injection of glycerol (50%, 10 ml/ kg).The total period of observation after glycerol injection was 8 hours.Hematocrit and plasma osmolality increased transiently and reached maximum values between 1 and 2 hours; plasma urea concentration rose progressively during the 8-hour period.Plasma levels of arginine-vasopressin had increased 40-fold by 2 hours after glycerol injection, whereas plasma renin concentrations were only 2-3 times higher than in controls.Plasma renin substrate concentrations had doubled by 8 hours.In rats with acute renal failure, blood pressure was higher than in controls injected with saline.Injection of vasopressin antiserum lowered blood pressure by 10 ± 2 (SE) mm Hg, while a competitive antagonist of angiotensin II, saralasin, had no effect.It is concluded that after glycerol injection the increased plasma concentrations of vasopressin induced systemic vasoconslriction.The renin-angiotensin system does not significantly contribute to the rise in systemic vascular resistance.IN ACUTE RENAL failure induced by the injection of glycerol, renal blood flow is markedly reduced. 1 " 3 In addition to intrarenal mechanisms, 4 ' 5 cardiovascular changes also might be responsible for the impairment of renal hemodynamics and excretory function.Blood pressure is not lowered in this experimental model, 6 despite a decrease in plasma volume 7 and cardiac output; 8 the mechanisms underlying these changes in the systemic circulation have not yet been investigated.In the present study we have analyzed the early phase of glycerol-induced acute renal failure.Since in former investigations the renin-angiotensin system has been considered to be of pathogenetic significance for the increase in intrarenal resistance , 9 " 12 the plasma concentrations of renin and its substrate were measured.The accumulating evidence for a vasoconstrictor role of antidiuretic hormone in shock and hypovolemia 13 " 15 prompted us to determine the plasma levels of arginine-vasopressin.By means of specific blockade, attempts were made to establish the role of angiotensin II and arginine-vasopressin in the maintenance of blood pressure during the development of glycerol-induced acute renal failure. Methods GENERAL EXPERIMENTAL PROTOCOLMale Sprague-Dawley rats (SIV-50 strain, Ivanovas) weighing 180-240 g were kept at constant temperature (23 ± 1°C) and humidity (60 ± 3%) in a room lighted automatically from 6 a.m. to 6 p.m.They were given a commercial diet (ssniff) and demineralized water ad libitum.
THE LONG-TERM EFFECTS OF AEROBIC TRAINING VERSUS COMBINED TRAINING ON PHYSICAL FITNESS AND CARDIOVASCULAR DISEASES RISK FACTORS IN OVERWEIGH ELDERLY MEN WITH HIGH BLOOD PRESSURE
Authors: Nelson Sousa, Romeu Mendes, Catarina Abrantes, Jaime Sampaio, José Oliveira
Journal: British Journal of Sports Medicine (2013)

Background Risk factors of cardiovascular diseases (CVD) tend to aggregate with aging. Several studies showed the effectiveness of exercise on physical fitness and cardiovascular health; however, research on the long-term effects of different modes of exercise in subjects at risk is still lacking. Therefore, the purpose of this study was to compare different modes of exercise on physical fitness and CVD risk factors in overweigh elderly men with high blood pressure. Methods Forty-eight overweigh elderly men (BMI≥25 kg/m2) with high blood pressure (SBP≥130 and/or DBP≥85 mmHg) were randomly assigned to an aerobic training group (ATG, n=15, 71.7±4.7 years), a combined aerobic and resistance training group (CTG, n=16, 68.5±3.5 years), and a control group (n=17, 67.0±5.8 years). Both training programs were of moderate-to-vigorous intensity, three days per week for nine months. Six independent physical fitness tests (back scratch, chair sit-and-reach, 30-s chair stand, arm curl, 8-foot up-and-go, 6-min walk), body fat mass, fat-free mass, systolic and diastolic blood pressure were measured on five different occasions. The data were analyzed using a mixed-model analysis of variance. Results ANOVA indicated a significant main effect of group (p≤0.001) for all physical fitness tests, with significant differences between both training groups and control. However the ATG only improved flexibility (back scratch and chair sit-and-reach), whereas CTG improved all physical fitness components. ANOVA also identified a significant main effect of time for 8-foot up-and-go (p=0.031) in the CTG. A significant main effect of group (p=0.000) and time (p=0.029) was observed in body fat mass percentage, with a 2.3% decrease in CTG. A significant main effect of time was observed in SBP (p=0.005) with reductions of 15 mmHg and 24 mmHg (in ATG and CTG, respectively) and in DBP (p=0.011) with reductions of 6 mmHg and 12 mm Hg (in ATG and CTG, respectively). Conclusions Both training programs increased physical fitness, however the effects are more extensive with the combination of aerobic and resistance training. exercise training is a non-pharmacological safe and effective tool in reducing blood pressure, but larger changes are observed in the presence of body fat loss. Therefore, the combination of aerobic training with resistance training in the same program is more effective in the promotion of physical fitness and in the prevention of CVD risk factors than aerobic training alone.
Experimental Coronary Occlusion in Dogs and Its Effect Upon the Cardiac Glycogen Fractions
Authors: ARTHUR W. MERRICK
Journal: Circulation Research (1957)

A chemical quantitative analysis of the acid-soluble and acid-insoluble fractions of myocardial glycogen following an episode of acute infarction is presented. Both glycogen forms are depleted from the more seriously occluded area. The labile fraction similarly is decreased in the ischemic zone surrounding the infarct while the acid-insoluble moiety maintains a normal level.
58 Hepatocarcinoma cells stimulate proliferation and migration and inhibit fibrogenic actions in human hepatic stellate cells: Regulation by hepatitis C virus proteins
Authors: P. Sancho-Bru, R. Bataller, E. Juez, V. Khurdayan, M. Moreno, J. Colmenero, V. Arroyo, D.A. Brenner, P. Ginès
Journal: Journal of Hepatology (2006)
Hybrid natural orifice transluminal endoscopic surgery: endoscopic full-thickness resection of early gastric cancer and laparoscopic regional lymph node dissection - 14 human cases
Authors: W. Cho, Y. Kim, J. Cho, G. Bok, S. Jin, T. Lee, H. Kim, J. Kim, J. Lee
Journal: Endoscopy (2010)

See also: Commentaire de travail de W. Y. Cho et al., pp. 134Endoscopy 2011; 43(02): 174-175DOI: 10.1055/s-0031-1291818
Prosper Menière's Syndrome and Disease
Authors: Henry L. Williams
Journal: JAMA (1965)

<h3>To the Editor:—</h3> I was amused lately to have sought Dr. Gordon Gilbert's communication inThe Journal(<b>191</b>:691-694 [March 1] 1965). I found it quite easily. In the subject index in the March 29 issue it was plainly listed under the heading "Ménière's Disease." The article itself is entitled "Meniere's Syndrome." In an exchange of correspondence Dr. Gilbert told me that in his original article he had used "Menière's disease," but that owing to importunities by the editorial department ofThe Journal, he had reluctantly changed the term to Menière's Syndrome. Aside from the incongruousness of the index department's not knowing what the editorial department does, I do not believe that the AMA book of nomenclature is clear in its use. Stothers<sup>1</sup>discusses the issue: A syndrome is defined as a composite group of signs and symptoms which occur in combinations sufficiently often to be recognized as an
Nondetected Tumor (pT0) after Prolonged, Neoadjuvant Treatment of Localized Prostatic Carcinoma
Authors: J. K&ouml;llermann, U. Feek, H. M&uuml;ller, U. Kaulfuss, U. Oehler, B. Helpap, M.W. K&ouml;llermann
Journal: European Urology (2000)
Abstract 2468: A p53 and microRNA-mediated pathway regulates mesenchymal stem cell differentiation
Authors: Yunlong He, Shunlin Jiang, Wendy Dubois, Ling Ren, Chand Khanna, Jing Huang
Journal: Cancer Research (2014)

Abstract The tumor suppressor p53 plays important roles in bone density regulation and osteosarcoma suppression. However, the relationship between p53-mediated bone density regulation and osteosarcoma suppression is unclear mainly because the underlying molecular and cellular mechanisms are elusive. Here, we reported that p53 controls differentiation of mouse mesenchymal stem cells (mMSCs) in a cell cycle and apoptosis independent manner. The loss of p53 predisposes mMSCs towards osteogenic lineage but not adipogenic and chondrogenic lineages. This pro-osteogenic effect caused by p53 loss is associated with the up-regulation of Runt related transcription factor 2, a factor required for the osteogenic differentiation of mMSCs. Without extrinstic stress, studies show that p53 does not directly repress the transcription of Runt related transcription factor 2. Instead, it indirectly represses Runt related transcription factor 2 through activating microRNAs (miRs), which suppress Runt related transcription factor 2 at a post-transcriptional level. In osteosarcoma cells, the loss of p53 correlates with high levels of Runt related transcription factor 2 and the oncogene addiction of these cells to Runt related transcription factor 2. Together, our results discover a p53 and miR-mediated pathway regulating the differentiation of MSCs and this pathway is dysregulated in osteosarcoma cells. This lineage controlling role links the role of p53 in the osteogenic differentiation of MSC to its suppressive functions in osteosarcoma. Citation Format: Yunlong He, Shunlin Jiang, Wendy Dubois, Ling Ren, Chand Khanna, Jing Huang. A p53 and microRNA-mediated pathway regulates mesenchymal stem cell differentiation. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2468. doi:10.1158/1538-7445.AM2014-2468
Managing Liability Risk in the Office Laboratory
Authors: Richard Belsey
Journal: JAMA (1986)

New technology has created new responsibilities and new risks for office practitioners who develop and use an office laboratory. Mistakes are bound to occur, but a system organized along the lines suggested is a workable way to minimize the number of mistakes and lessen the consequences of any mistakes that do occur. If a significant problem occurs and these guidelines have been ignored, it is likely they will be used as a framework for inquiry. The benefit to the physician and patient far outweighs the risk of using the new technology and procedures. Any risk is manageable because the physician has the opportunity to control the technology selected, the procedures and protocols used, and the persons involved in the testing process. (<i>JAMA</i>1986;256:1338-1341)