Heat Inactivation in the Study of Human Alkaline Phosphatases
Authors: SOLOMON POSEN
Journal: Annals of Internal Medicine (1965)

Article1 June 1965Heat Inactivation in the Study of Human Alkaline PhosphatasesSOLOMON POSEN, M.B., FRANCIS C. NEALE, PH.D., JOHN S. CLUBB, M.B.SOLOMON POSEN, M.B.Search for more papers by this author, FRANCIS C. NEALE, PH.D.Search for more papers by this author, JOHN S. CLUBB, M.B.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-62-6-1234 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptOver the past few years, several attempts have been made to identify the sources of human serum alkaline phosphatase (or phosphatases) by immunological (1), electrophoretic (2-4), or chemical (5) techniques. The methods employed are frequently too cumbersome for routine clinical use, while the results in many instances fail to answer whether, in a given patient, alkaline phosphatase is of hepatic, bony, or other origin. Indeed, some workers (6) have doubted the existence of consistent qualitative differences between the serum enzyme of patients with skeletal disorders and that of patients with hepatic disease and have attributed serum alkaline phosphatase elevation in...References1. SCHLAMOWITZBODANSKY MO: Tissue sources of human serum alkaline phosphatase as determined by immunochemical procedures. J. Biol. Chem. 234: 1433, 1959. CrossrefMedlineGoogle Scholar2. CHIANDUSSIGREENESHERLOCK LSFS: Serum alkaline phosphatase fractions in hepato-biliary and bone diseases. Clin. Sci. 22: 425, 1962. MedlineGoogle Scholar3. BOYER SH: Human organ alkaline phosphatases: discrimination by several means including starch gel electrophoresis of antienzyme— enzyme supernatant fluids. Ann. N. Y. Acad. Sci. 103: 938, 1963. CrossrefMedlineGoogle Scholar4. HAIJEDE JONG WGM: Isoenzyme patterns of serum alkaline phosphatase in agar-gel electrophoresis and their clinical significance. Clin. Chim. Acta 8: 620, 1963. CrossrefMedlineGoogle Scholar5. PEACOCKREEDHIGHSMITH ACRAEM: Ethanol fractionation of human serum alkaline phosphatase. Clin. Chim. Acta 8: 914, 1963. CrossrefMedlineGoogle Scholar6. GUTMAN AB: Serum alkaline phosphatase activity in diseases of the skeletal and hepatobiliary systems: a consideration of the current status. Amer. J. Med. 27: 875, 1959. CrossrefMedlineGoogle Scholar7. DALGAARD JB: Serum phosphatase after hepatectomy in dogs. Acta Physiol. Scand. 16: 308, 1949. CrossrefMedlineGoogle Scholar8. NEALECLUBBHOTCHKISPOSEN FCJSDS: The heat stability of human placental alkaline phosphatase. J. Clin. Path. In press. Google Scholar9. MCMASTERTENNANTCLUBBNEALEPOSEN YRJSFCS: The mechanism of the elevation of serum alkaline phosphatase in pregnancy. J. Obstet. Gynaec. Brit. Comm. 71: 735, 1964. CrossrefMedlineGoogle Scholar10. STEINBACH HL: Some roentgen features of Paget's disease. Amer. J. Roentgen. 86: 950, 1961. Google Scholar11. ALBRIGHTREIFENSTEIN FEC: The Parathyroid Glands and Metabolic Bone Disease. The Williams and Wilkins, Baltimore, 1948, p. 205. Google Scholar12. SISSONS HA: Histological changes encountered in some generalized skeletal diseases. Proc. Roy. Soc. Med. 51: 485, 1958. MedlineGoogle Scholar13. POSEN S: Renal osteodystrophy. Med. J. Aust. 2: 264, 1964. CrossrefMedlineGoogle Scholar14. KINGARMSTRONG EJAR: Convenient method for determining serum and bile phosphatase activity. Canad. Med. Ass. J. 31: 376, 1934. MedlineGoogle Scholar15. MOSSKING DWEJ: Properties of alkaline phosphatase fractions separated by starch-gel electrophoresis. Biochem. J. 84: 192, 1962. CrossrefMedlineGoogle Scholar16. BODANSKY O: Are the phosphatases of bone, kidney, intestine and serum identical? The use of bile acids in their differentiation. J. Biol. Chem. 118: 341, 1937. CrossrefGoogle Scholar17. BURKE JO: Serum alkaline phosphatase in liver disease. A concept of its significance. Gastroenterology 16: 660, 1950. CrossrefMedlineGoogle Scholar18. HEPPELHARKNESSHILMOE LADRRJ: A study of the substrate specificity and other properties of the alkaline phosphatase of Escherichia coli. J. Biol. Chem. 237: 841, 1962. CrossrefMedlineGoogle Scholar19. POSENCLUBBNEALEHOTCHKIS SJSFCD: The measurement of plasma volume by enzyme dilution. J. Lab. Clin. Med. 65: 530, 1965. MedlineGoogle Scholar20. BIRNSMASEKAUERBACH MBO: The effects of acute biliary obstruction and release on the rat liver. Amer. J. Path. 40: 95, 1962. MedlineGoogle Scholar21. CLUBBNEALEPOSEN JSFCS: The behaviour of infused placental alkaline phosphatase in human subjects. J. Lab. Clin. Med. in press. Google Scholar22. SHERLOCK S: Jaundice. Brit. Med. J. 1: 1359, 1962. CrossrefMedlineGoogle Scholar23. COHENFREEMANMCFARLANE STAS: Metabolism of 131I labelled human albumin. Clin. Sci. 20: 161, 1961. MedlineGoogle Scholar24. JEEJEEBHOY KN: Cause of hypoalbuminaemia in patients with gastrointestinal and cardiac disease. Lancet 1: 343, 1962. CrossrefMedlineGoogle Scholar25. POLINSPELLBERGTEITELMANOKUMARA SGMALM: The origin of elevation of serum alkaline phosphatase in hepatic disease. Gastroenterology 42: 431, 1962. CrossrefMedlineGoogle Scholar26. BRAUER RW: Mechanisms of bile secretion. JAMA 169: 1462, 1959. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Sydney, N.S.W.,From the Department of Medicine, University of Sydney, and the Department of Biochemistry, Sydney Hospital, Sydney, N.S.W., Australia.This work was supported by the Post-Graduate Medical Foundation, University of Sydney, Messrs. Farmer Hill Pty. Ltd., and Messrs. Geigy (Australasia) Ltd., Sydney, N.S.W., Australia.Requests for reprints should be addressed to Solomon Posen, M.B., Department of Medicine, Sydney Hospital, Sydney, N.S.W., Australia. 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