tbuckley/cabot-search huggingface dataset:
Abstract 4727: Prognostic roles of DDB2 and XPC mRNA expressions in treatment responses and overall survivals of head and neck cancer patients
Authors: Chang-Shen Lin, Ka-Wo Lee, Jau-Ling Huang, Yu-Chu Wang, Yi-Shan Tsai
Journal: Cancer Research (2014)

Abstract Background: DNA repair genes function in the maintenance of genome integrity and prevent tumorigenesis. On another hand, DNA repair genes modulate cancer cell responses to DNA-damaging therapeutics. We hypothesize that altered expression of DNA repair genes are correlated with therapeutic outcomes of head and neck cancer patients. Patients and methods: Messenger RNA (mRNA) expressions of DDB2 and XPC were examined in specimens of 95 head and neck cancer patients using real-time quantitative RT-PCR. Patients were dichotomized by ROC curve analysis based on their DDB2 and XPC mRNA levels. Overall survival (OS) rates were measured using Kaplan-Meier estimates and the log-rank tests. The adjusted hazard rate ratios (HRRs) were computed by multivariate Cox regressions. Results: The levels of DDB2 and XPC mRNA expression in tumor tissues were reduced (tumor/normal &amp;lt; 0.9) in 84 and 80 cases, respectively, when compared with those in adjacent normal parts. Lower DDB2 (tumor/normal &amp;lt; 0.24) and XPC (tumor/normal &amp;lt; 0.36) mRNA expressions were correlated with larger tumor, positive lymph node invasion, advanced stage, and poor 5-yr OS rates (26.7% vs. 54.3%, and 28.1% vs. 53.0%, respectively). Multivariate Cox model showed that, in addition to T and N stages, lower XPC mRNA level (&amp;lt; 0.36) was an independent risk factor for patient's survival (HRR = 2.68, 95% CI 1.12-6.40). Concurrent chemotherapy and radiotherapy (CCRT) did not improve OS of patients with lower XPC mRNA (&amp;lt; 0.36) and showed even worse outcomes within 5 years for patients with lower DDB2 expression (&amp;lt; 0.24). However, patients with higher DDB2 (&amp;gt; 0.24) and XPC (&amp;gt; 0.36) mRNA levels had improved 5-yr OS rates if they received CCRT (64.4% vs. 41.8% and 73.3% vs. 39.2%). Conclusion: mRNA expressions of XPC and DDB2 might be useful biomarkers for prognosis and efficacy of CCRT medication in head and neck cancer patients. Citation Format: Chang-Shen Lin, Ka-Wo Lee, Jau-Ling Huang, Yu-Chu Wang, Yi-Shan Tsai. Prognostic roles of DDB2 and XPC mRNA expressions in treatment responses and overall survivals of head and neck cancer patients. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4727. doi:10.1158/1538-7445.AM2014-4727
A STUDY OF CONDITIONS FOR THE OPTIMUM PRODUCTION OF PR8 INFLUENZA VIRUS IN CHICK EMBRYOS
Authors: Gail Lorenz Miller
Journal: The Journal of Experimental Medicine (1944)

In order to determine the conditions for the optimum production of PR8 influenza virus in chick embryos, a study has been made of the róles of concentration of virus in the inoculum, temperature of incubation of infected embryos, length of time of incubation of infected embryos, and age of embryos at the time of inoculation. Relative amounts of virus in different preparations were measured indirectly by means of determinations of chicken red blood cell agglutination titers. Frozen infectious allantoic fluid which produced infection in chick embryos at a maximum dilution of 10(-7) was employed as a stock inoculum. Best results were obtained with an amount of stock inoculum of 0.1 cc. of a 10(-5) dilution, a temperature of incubation of 35 degrees C., a length of time of incubation of 36 to 48 hours, and with embryos brought to 10 or 11 days of age at 37 degrees C. or 9 or 10 days of age at 39 degrees . An uncontrolled factor arising from inherent variations in the properties of different embryos and different batches of embryos was discussed.
Decreased apoptosis and distinct profile of infiltrating cells in the nasal polyps of patients with aspirin hypersensitivity
Authors: M. L. Kowalski, J. Grzegorczyk, R. Pawliczak, T. Kornatowski, M. Wagrowska‐Danilewicz, M. Danilewicz
Journal: Allergy (2002)

Background: Patients with aspirin‐hypersensitive rhinosinusitis/asthma suffer from a severe form of hyperplastic rhinosinusitis with recurrent polyposis. We aimed to assess the presence of apoptotic cells in nasal polyps from aspirin‐hypersensitive (AH) and aspirin‐tolerant (AT) patients with rhinosinusitis as related to the characteristics of local inflammation. Methods: Nasal polyps obtained from 16 AH patients and 36 AT patients (17 atopic and 19 nonatopic) were stained for eosinophils and metachromatic cells, and in parallel immunocytochemistry was performed to detect CD45RO+, HLA‐DR+, CD8+ and CD68+ positive cells. Apoptotic cells were detected by a nick‐end labelling technique, TUNEL. Results: The density of apoptotic cells in AH polyps (5.5 + 1.5 cells/mm 2 ) was significantly lower as compared to both atopic (18.7 + 3.8 cells/mm 2 ; P &lt; 0.02;) and nonatopic (21.3 + 5.2 cells/mm 2 ; P &lt; 0.01) AT polyps. The number of eosinophils, mast cells, and CD45RO+ cells were significantly increased in AH compared to AT polyps ( P &lt; 0.001), and the density of HLA‐DR+ cells in AH patients was higher than in nonatopic ( P &lt; 0.02), but not in atopic AT patients. While in AH patients the duration of rhinosinusitis correlated inversely with the number of apoptotic cells ( r = − 0.67; P &lt; 0.04), in contrast, in AT atopic patients the duration of rhinosinusitis showed positive correlation with apoptosis ( r = 0.89; P &lt; 0.003). Conclusions: We conclude, that decreased apoptosis of inflammatory cells in nasal polyps from ASA‐hypersensitive patients, reflects a distinct mechanisms of local inflammation and may be related to persistence and severity of the disease in these patients.
HIV-1 infection impairs cell cycle progression of CD4+ T cells without affecting early activation responses
Authors: Scott F. Sieg, Clifford V. Harding, Michael M. Lederman
Journal: Journal of Clinical Investigation (2001)

Failure of CD4+ T cells to proliferate in response to antigenic stimulation is a characteristic of HIV infection. Analysis of the proliferation defect has been hampered by an inability to identify CD4+ cells with T cell receptor specificity for antigen. To focus only on cells that had been stimulated through the T cell receptor, CD4+ T cells were stimulated with an anti-Vβ3 Ab that activates approximately 3–5% of peripheral blood T cells. This approach revealed proliferation defects in cells from HIV-infected patients that were not appreciated using anti-CD3 Ab stimulation and provided the capacity to examine responses on a single cell basis. After anti-Vβ3 Ab stimulation, CD4+Vβ3+ cells from HIV-infected patients demonstrated defects in expression of cell cycle–associated proteins, D-type cyclins, and cyclin A. However, the expression of early activation markers, CD69 and CD25, was not significantly impaired in cells from most patients. Thus, CD4+ T cell proliferation failure in HIV disease is characterized by dysregulated activation that precludes cell cycle progression. This proliferation defect was most apparent in patients with diminished CD4+ T cell numbers and higher plasma HIV RNA levels. CD4+ T cell proliferation failure may be a key determinant of immune impairment in HIV disease.
Abstract 16625: Neuronal Sodium Channels Contribute Significantly to Cardiac Repolarization During Ischemia
Authors: Michael Biet, Anh-Tuan Ton, Jean-Francois Delabre, Nathalie Morin, Robert Dumaine
Journal: Circulation (2011)

Introduction: It has been demonstrated that neuronal sodium channels (nNavs) are overexpressed around infracted areas and during ischemia. The role of nNavs expression during cardiac ischemia is st...
Echocardiographic assessment of the level of cardiac compensation in valvular heart disease.
Authors: A Rosenblatt, R Clark, J Burgess, K Cohn
Journal: Circulation (1976)

The level of cardiac compensation in valvular disease was studied by relating echocardiographic and cardiac catheterization measurements. Three groups -- compensated, intermediately compensated, and decompensated -- were defined according to the left ventricular angiographic pattern and cardiac output. The echocardiographic ejection indices, percent left ventricular minor diameter shortening, ejection fraction, and fiber shortening rate were significantly higher than normal in compensated mitral regurgitation, lower then normal in compensated aortic stenosis, and within normal limits in compensated aortic insufficiency. In the decomposed state these indices were depressed. Intermediate compensation was best recognized by combining several echocardiographic variables into an echocardiographic score based on multivariate discriminant function analysis. Thus, the compensated volume overload states (aortic and mitral regurgitation) and pressure overload state (aortic stenosis) have separate sets of "normal" echocardiographic values; low ejection indices characterize the decompensated group, while recognition of intermediate compensation requires analysis of multiple echocardiographic variables.
Value of Long-Term Anticoagulant Therapy in Coronary Disease
Authors: A. B. Thomes
Journal: JAMA (1961)

One hundred fifty-six patients with coronary disease were observed on an initial uninterrupted course of anticoagulant therapy. Of these, 84 were observed after discontinuation of treatment. First, nonfatal recurrence rates and death rates were determined at successive time periods by the survivorship table method. An estimated 803 per 1,000 patients on continuous therapy remained recurrence-free after 60 months compared with 365 per 1,000 remaining recurrence-free for 60 months after discontinuance of treatment. This difference was shown to be statistically significant throughout the period of observation. The recurrence rate after discontinuance is higher in those with more prolonged initial treatment, suggesting the inadvisability of stopping treatment. An estimated 736 per 1,000 survived after 60 months of treatment. Exclusion of patients with diabetes and heart failure increased the 5-year survival rate to 912 per 1,000.
THE DIAGNOSIS OF HEART DISEASE IN CHILDREN.
Authors: J. P. CROZER GRIFFITH
Journal: JAMA (1900)

This paper has been prepared on such short notice as to give me no opportunity to consult medical journals and text-book literature. What I have to say is, therefore, purely the result of my own experience with affections of the heart in children. The first question which naturally arises in diagnosis is, is heart disease present, and if so, is it congenital or postnatal? Let us take up first the study of congenital heart disease. The principal diagnostic symptoms of this condition are: cyanosis, clubbing of the fingers, thrill, characteristic murmurs, the absence of any great enlargement of the heart. Cyanosis is peculiarly marked in congenital heart disease; in fact, I do not know of any other condition in which it is so intense. Even in severe forms of postnatal heart disease, with entire lack of compensation and decided blueness of the lips, I never have seen the blue-red tongue,
Short Term Outcomes of High Power (80 W) Potassium-titanyl-phosphate Laser Vaporization of the Prostate
Authors: Tugcu Volkan, Tasci Ali Ihsan, Ordekci Yilmaz, Ozbek Emin, Sahin Selcuk, Kantay Koray, Ozbay Bedi
Journal: European Urology (2005)
Pneumatosis intestinalis. A complication of jejunoileal bypass
Authors: S. N. Martyak
Journal: JAMA (1976)

AS MORE experience is gained with jejunoileal bypass, more unanticipated complications are being discovered. A complication not yet reported is pneumatosis intestinalis in the defunctionalized small-bowel segment. This complication occurred in one patient in our series. <h3>Report of a Case</h3> A 46-year-old man weighing 154.5 kg (340 lb) underwent jejunoileal bypass for morbid obesity. He had prior episodes of severe cardiopulmonary failure. No endocrine abnormalities were discovered during preoperative evaluation. Upper gastrointestinal and small-bowel roentgenographic series, barium enema examination, and oral cholecystogram were normal. After treatment of the cardiac and pulmonary failure with digitalis, diuretics, and respirator therapy, the patient underwent a jejunoileal bypass with 35.5 cm of jejunum, anastomosed end-to-end to the terminal 10 cm of ileum. The defunctionalized small-bowel segment was closed proximally and fixed to the root of the small-bowel mesentery, and the distal segment anastomosed end-to-side to the sigmoid colon. The postoperative course was complicated by
Partial Pituitary Ablation with Implants of Gold-198 and Yttrium-90 for Acromegaly
Authors: M. Hartog, F. Doyle, R. Fraser, G. F. Joplin
Journal: BMJ (1965)

The ideal method for the treatment of acromegaly remains uncertain. Evidence of local pressure from a pituitary tumour offers an absolute indication for treatment, and this has usually been surgical decompression ; however, when treatment has been given by external deep x-ray therapy the majority of such patients have shown improvement in their visual-field defects (Fraser and Joplin, 1961). Where the presenting syndrome comprises only acromegaly and the other effects of excessive growth-hormone secretion the need for treatment may seem less obvious. The activity of acromegaly is difficult to assess, and the natural history of the untreated disease is not adequately known. Bishop and Briggs (1958) reported that 80 % of their patients had died by the age of 60 predominantly from cardiovascular complications, but this high mortality rate awaits confirmation. Acromegaly without symptoms of a pituitary tumour has often been left untreated ; however, we have implanted such cases as well as those with tumour signs or symptoms, so long as no evidence was found of any considerable extrasellar extension.
Analysis shows "postcode lottery" in access to GPs in England
Authors: I. Torjesen
Journal: BMJ (2014)

Patients in some of the most deprived areas of England find it four times as hard to get an appointment with their GP as patients living in more affluent areas, an analysis by the Royal College of General Practitioners has found. And the areas where making an appointment is hardest also tend to have the fewest GPs, it showed.

RCGP researchers compared data from the GP Patient Survey on how easy patients found it to make an appointment with their GP with levels of social deprivation and the number of GPs in each clinical commissioning group (CCG) area. They found that the patients most likely to find it difficult to make an appointment tended to live in more deprived areas and to have the lowest number of full time equivalent GPs …
Epileptic seizures in a population of 6000. II: Treatment and prognosis.
Authors: D M Goodridge, S D Shorvon
Journal: BMJ (1983)

Treatment and prognosis were studied in 122 patients with non-febrile seizures in a population of 6000. Phenytoin and phenobarbitone were the most commonly prescribed drugs, although the popularity of phenobarbitone had declined over time. The average duration of treatment was relatively short, and most patients received single drug treatment. Treatment patterns were erratic, and the surveillance and audit of treatment generally poor. Recurrence after a first attack was found in four fifths of the patients. Generally the total number of seizures suffered by each patient was small, the period of active epilepsy short, and remission when it occurred was usually permanent. The cumulative probability of continuing activity fell and the proportion of patients in remission rose over time. Patients with partial or mixed seizure types had a poorer overall prognosis. The course of the epilepsy in the early years of treatment proved to be a useful guide to the long term prognosis, and the possibility that effective treatment might influence long term prognosis is raised.
A standardised method of estimating KCO on exercise.
Authors: E Neville, A H Kendrick, G J Gibson
Journal: Thorax (1984)

This study was designed to standardise a progressive exercise test for the assessment of change in carbon monoxide transfer coefficient (KCO) with exercise and to examine the variation between subjects and the reproducibility within subjects. Normal subjects exercised on a bicycle ergometer while ventilation, heart rate, and expired gas concentrations were recorded continuously. Preliminary studies showed that reduction of the breath hold time to six seconds made measurements of KCO during heavy exercise more comfortable without affecting the result. When KCO was measured immediately after exercise it was lower than when measured during exercise. KCO was measured in 50 normal subjects at rest and at three different work loads maintained for three minutes with a pause of five minutes between each. The relationships between KCO and both oxygen consumption and work load were linear in all subjects but the relationship between KCO and heart rate was distorted by high resting heart rates in some subjects. The mean slope of the relationship between KCO and oxygen consumption (VO2) was steeper in women than in men (mean slopes 0.627 and 0.348 mmol min-1 kPa-1 l-1 per 1 min-1 respectively), and the same was true for the relationship between KCO and work rate. The heart rate rose more steeply in relation to VO2 in women, so that the relationship of KCO to heart rate was similar in men and women (mean slope 0.01 mmol min-1 kPa-1 l-1 per beat min-1). Repeat studies on five occasions in five individuals gave coefficients of variation for the slopes of the relationships between KCO and VO2, work rate, and heart rate of 5-10%.
Interobserver reliability in measuring flexion, internal rotation, and external rotation of the hip using a plurimeter.
Authors: P R Croft, E S Nahit, G J Macfarlane, A J Silman
Journal: Annals of the Rheumatic Diseases (1996)

To determine reliability of the measurement of hip movements (flexion, internal rotation, and external rotation) between medical practitioners.Six clinicians carried out measurements of hip movements on each of six patients with osteoarthritis of one hip, using a specifically designed plurimeter.There was no evidence of any systematic difference between medical practitioners in the measurement of hip flexion, internal rotation, or external rotation. The degree of agreement was greatest for hip flexion.This study has shown that measurement of range of movement at the hip is repeatable between practitioners using a simple plurimeter and may represent an examination that is suitable for monitoring progress and treatment.
Matrix metalloproteinase-2 activation in human hepatic fibrosis regulation by cell-matrix interactions
Authors: Anne-Marie Préaux, Ariane Mallat, Jeanne Tran Van Nhieu, Marie-Pia d'Ortho, Rosalind M. Hembry, Philippe Mavier
Journal: Hepatology (1999)

Matrix metalloproteinase-2 (MMP-2) is involved in extracellular matrix remodeling. It is secreted as a proenzyme and activated by membrane type-MMPs (MT-MMP), such as MT1-MMP. In liver fibrosis, MMP-2 is highly expressed in myofibroblasts and may have a profibrogenic role. The mechanisms of its activation in the liver are still unclear. The aim of this work was to show that pro-MMP-2 is efficiently activated in human fibrotic liver and to investigate the role of cell-matrix interactions in this process. Liver specimens obtained from patients with active cirrhosis were compared to normal liver specimens. Human hepatic myofibroblasts were cultured either on plastic, fibronectin, laminin, or on collagen I gels. MMP-2 activity was visualized by gelatin zymography. MMP-2 active form (59 kd) was detected in active cirrhosis but not in normal liver. Myofibroblasts cultured on plastic, fibronectin, or laminin predominantly expressed inactive pro-MMP-2 (66 kd). In contrast, myofibroblasts cultured on collagen I markedly activated the enzyme. Similar results were obtained using membrane fractions from cells previously cultured on collagen or plastic. Activation was inhibited by the tissue inhibitor of metalloproteinases-2 but not by tissue inhibitor of metalloproteinases-1, implicating a MT-MMP-mediated process. Culture on collagen I up-regulated MT1-MMP protein detected by Western blotting, but decreased MT1-MMP mRNA. This study shows that MMP-2 is activated in fibrotic liver. It suggests that interactions between collagen I and myofibroblasts promote this process through a post-translational increase of MT1-MMP expression in these cells.
APPARATUS FOR IMMOBILIZING UPPER EXTREMITY DURING INTRAVENOUS THERAPY
Authors: Lawrence J. Knox
Journal: JAMA (1952)

The majority of patients in general medical and surgical hospitals receive some type of intravenous medication. The technical success of the administration of fluid depends to a large extent on the proper immobilization of the upper extremity. Flexion at the elbow and pronation and supination of the forearm are the movements that displace the needle. Either the needle is withdrawn from the vein or the needle is driven deeper in the tissues. In both cases formation of a hematoma is frequent and reinsertion of the needle is necessary usually at a different site. Watching the patient during intravenous administration does not solve the problem, because a conscious, partially conscious, or comatose patient will displace the needle in a sudden fast movement before the attendant can stop him. This is especially true of agitated patients or those coming out from under an anesthetic, following surgery, or patients in a state of
Medical Management of Cardiac Transplants.
Authors: Robert D. Leachman
Journal: Annals of Internal Medicine (1969)

s1 May 1969Medical Management of Cardiac Transplants.Robert D. Leachman, M.D., F.A.C.P., Louis L. Leatherman, M.D., Donald G. Rochelle, M.D., John R. Montgomery, M.D., Gerald M. Lemole, M.D.Robert D. Leachman, M.D., F.A.C.P.Search for more papers by this author, Louis L. Leatherman, M.D.Search for more papers by this author, Donald G. Rochelle, M.D.Search for more papers by this author, John R. Montgomery, M.D.Search for more papers by this author, Gerald M. Lemole, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-70-5-1078_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTransplantation of the human heart is now a A possible means of therapy for those patients with advanced heart disease that is not amenable to other forms of therapy. This paper is based on experience gained in the clinical management of 14 adult heart transplant patients.The preferred donor is one who has sustained a fatal head injury, intracerebral hemorrhage, or is in the terminal stage of a localized intracerebral neoplasm. Careful assessment of the potential recipient's renal, hepatic, pulmonary, and bone marrow status is carried out before transplantation. Cultures of the skin, urine, stool, and throat are also performed.... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: Robert D. Leachman, M.D., F.A.C.P.; Louis L. Leatherman, M.D.; Donald G. Rochelle, M.D.; John R. Montgomery, M.D.; Gerald M. Lemole, M.D.Affiliations: Houston, Texas PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 May 1969Volume 70, Issue 5Page: 1078-1078KeywordsBone marrowCardiac transplantationCardiovascular therapyHead injuryHeartHemorrhagePharynxTransplantationUrine ePublished: 1 December 2008 Issue Published: 1 May 1969 PDF downloadLoading ...