tbuckley/cabot-search huggingface dataset:
Abstract 1149: Genetic analysis of single cells
Authors: Jian-Bing Fan, Jing Chen, Craig April, Brandy Klotzle, Thomas Royce, Gordon Cann, AmirAli H. Talasaz, Saiful Islam, Una Kjallquist, Sten Linnarsson
Journal: Cancer Research (2010)

Abstract We are developing highly sensitive and quantitative genomic technologies for genetic and expression analysis of single cells or trace amount of DNA/RNA materials, using microarray and next-generation sequencing platforms. We have tested various Multiple Displacement Amplification (MDA)-based protocols under a variety of reaction conditions. With our current protocol and a 300K-SNP chip readout, we were able to obtain 88.3% and 93.9% call rate, and 97.4% and 99.9% call accuracy with direct cell lysis from 1 cell and 5 cells, respectively. We are also developing RNA amplification methods for high-throughput expression profiling of single cells. With our current protocol, we were able to generate reproducible expression profiles, R2 = 0.73 and 0.77, using 10 pg and 50 pg total RNA input, respectively. In addition, the profiles correlated well with those obtained with standard 100 ng total RNA input, R2 = 0.61 and 0.77, respectively. Our data show that sequencing of single-cell transcriptomes can clearly distinguish embryonic stem cells from embryonic fibroblasts and tumor cells. We are currently using these technologies to study medical specimens such as circulating tumor cells and cancer stem cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1149.
<i>Horizon</i> : Does the MMR Jab Cause Autism?: We cannot win
Authors: Neville W Goodman
Journal: BMJ (2005)

Calcium and vitamin D in preventing fractures Vitamin K supplementation has powerful effectEditor-Porthouse et al conducted a good randomised controlled trial of calcium and supplementation with cholecalciferol (vitamin D 3 ) for prevention of fractures in primary care. 1 However, vitamin D and calcium are not enough.No mention was made of the extensive research from Japan and the Netherlands, which shows that vitamin K supplementation has a powerful effect in decreasing osteoporosis and osteoporosis related fractures.Combining vitamin K, vitamin D, and calcium seems ideal.Researchers from Osaka Medical College showed that vitamin K and vitamin D together increased bone density much better than vitamin K alone. 2hen comparing calcium and vitamin D alone with placebo, researchers at the University of Maastricht found little benefit on bone loss.But those randomised to take vitamin K in addition to calcium and vitamin D had significantly less femoral neck bone loss after three years. 3he Yamaguchi osteoporosis prevention study showed that vitamin K alone reduced vertebral fractures by 56% compared to placebo, comparable to the benefit found from etidronate. 4 Researchers at Hirosaki University in Japan showed that vitamin K lowered bone fractures in elderly female patients with Parkinson's by 90%. 5 The same research team showed an 86% decrease in fractures in elderly patients with Alzheimer's treated with a combination of vitamin K, vitamin D, and calcium compared with placebo.w1 If the medical standard became to first use vitamins D and K with calcium before using bisphosphonates or selective oestrogen receptor modulators, the public would save billions of dollars a year.
Dickens and doctors: vignettes of Victorian medicine.
Authors: J. E. Cosnett
Journal: BMJ (1992)

broad outline of the picture.The next day, involving the most challenging part of the commission, painting the head and face, was the most trying day for me because I was unable to move my head without upsetting the pose.The artist often consulted the photographs and to my surprise used a tape measure to determine the relationships between various parts of my face.The technique of facial measurement derives from Holbein, the sixteenth century Dutch portraitist.Quick facial sketches are satisfactory for street corner artists using crayons on paper but not for a serious portrait.The artist's equipment consists of a palette and many brushes of various thicknesses, but most fasci- nating was his maulstick.This is a pole-like device that allowed him to maintain a steady hand for fine detail.The third day, spent painting the hands, was a mnore relaxed time for me as I could move my head, but I could not read until this part of the painting was completed.The next two days were spent painting the body and the clothes.These were easy days for me and it was satisfying to see progress in the painting after each session.After five days the painting was almost complete.Adding the finishing touches to the figure and painting the props and background took about two weeks, after I had returned home.The artist agreed that he would supervise the framing of the picture, and the lettering on the label was decided.There are only three profes- sional framers in London; framers are also responsible for varnishing the picture.Two months after the sitting the finished portrait arrived in Dublin.It was a pleasing likeness, even if the figure was somewhat portly.The sitting was made tolerable by the courtesy of the artist, whose cheerful manner kept me amused for five days.It was a learning experience, and I gained an artist as a friend.
Jaundice due to carbimazole.
Authors: M Lunzer, S N Huang, J Ginsburg, M Ahmed, S Sherlock
Journal: Gut (1975)

On three occasions, a 63 year old housewife with hyperthyroidism developed a reaction which included fever, pruritus, malaise, and, on one occasion, jaundice one to 17 days after taking carbimazole. Challenge with carbimazole was followed within 12 hours by abdominal pain, pruritis, and increased serum transaminase levels. Light microscopy of a liver biopsy showed increase of portal zone cellularity over the control and the electron microscopy revealed fine structural changes compatible with drug-related liver injury.
Lymphokines (MIF) in the serum of patients with sarcoidosis and cutaneous granuloma annulare
Authors: PABLO UMBERT, RICHARD W. BELCHER, R.K. WINKELMANN
Journal: British Journal of Dermatology (1976)

Serum MIF activity was studied in ten patients with sarcoidosis, fourteen with granuloma annulare, four with necrobiosis lipoidica, and nine with various dermatological diseases. Positive MIF activity was found in the sera of nine of the ten patients with sarcoidosis and eleven of the fourteen patients with granuloma annulare. The delayed hypersensitivity tests were negative in all nine of the patients with sarcoidosis who had serum MIF activity and were positive in only three patients with cutaneous sarcoid lesions. One of four patients with necrobiosis lipoidica demonstrated minimal serum MIF activity. Data on serum lymphokine activity in sarcoidosis and granuloma annulare suggest that these two diseases are related to delayed hypersensitivity mechanisms.
Comparison of the safety and efficacy of enoxaparin with unfractionated heparin and phenprocoumon as anticoagulation in cardioversion of nonvalvular atrial fibrillation
Authors: Uwe Nixdorff, Christoph Stellbrink, Thomas Hofmann, Peter Hanrath, Walter Lehmacher, Andre Schmidt-Lucke
Journal: Journal of the American College of Cardiology (2003)
Molecular remission and reversal of myelofibrosis in response to imatinib mesylate treatment in patients with the myeloproliferative variant of hypereosinophilic syndrome
Authors: Amy D. Klion, Jamie Robyn, Cem Akin, Pierre Noel, Margaret Brown, Melissa Law, Dean D. Metcalfe, Cynthia Dunbar, Thomas B. Nutman
Journal: Blood (2003)
An Improved Pappenheim Stain for Detection of Gonococci in Smears of Pus
Authors: M. M. Barritt
Journal: BMJ (1943)

Although the aetiology of the condition is not clear, further trial of sulphanilamide therapy is urged for the acute forms of lupus erythematosus without clinical evidence of tuberculosis.In view of the leucopenia accompanying the condition, the simultaneous administration of pentnucleotide seems to be indicated.
SPLENIC ENLARGEMENT IN HYPERTHYROIDISM
Authors: C. W. BALDRIDGE
Journal: JAMA (1927)

Splenic enlargement in hyperthyroidism has been very little mentioned in the voluminous literature on diseases of the thyroid gland. The occurrence of nodules, clumps and diffusely scattered lymphoid cells within the thyroid gland in exophthalmic goiter is an observation of long standing. A moderate lymphocytosis in the circulating blood of these cases has also been generally observed. MacCallum1speaks of a generalized hyperplasia of the fixed lymphatic tissue. Foster2includes the spleen in the generalized lymphoid reaction but does not convey an accurate notion as to the degree of splenic enlargement in exophthalmic goiter. Cameron and Sedziak3found that the feeding of thyroid extract to adult white rats resulted in a relative and absolute increase in the weight of most visceral organs. The increase in weight of the spleen and lymph nodes was proportionally more than that of other organs.    We have recently become impressed by the
Satisfied customers grow for NHS
Authors: C. Court
Journal: BMJ (1994)

Public dissatisfaction with the health service is falling steadily, according to the annual British survey of social attitudes. The number of people who believe that health should be the first priority for extra government spending has also fallen.

The survey shows that the proportion of people who were dissatisfied with the NHS fell to 38% in 1993, compared with 47% in 1990, when the health service market was poised to begin. The proportion of people expressing satisfaction with the NHS rose to 44% in 1993, compared with …
Arsenic trioxide-based therapy of relapsed acute promyelocytic leukemia: registry results from the European LeukemiaNet
Authors: E Lengfelder, F Lo-Coco, L Ades, P Montesinos, D Grimwade, B Kishore, S M Ramadan, M Pagoni, M Breccia, A J G Huerta, A M Nloga, J D González-Sanmiguel, A Schmidt, J-F Lambert, S Lehmann, E Di Bona, B Cassinat, W-K Hofmann, D Görlich, M-C Sauerland, P Fenaux, M Sanz
Journal: Leukemia (2015)
878-6 Stenting under intravascular ultrasound guidance of coronary bifurcation lesions with a new device allowing provisional side branch treatment
Authors: Wim J Vander Giessen, Jurgen R Ligthart, Pieter C Smiths, Pim J de Feyter, George Sianos, Thierry Lefèvre, Patrick W Serruys
Journal: Journal of the American College of Cardiology (2004)
The Safety and Efficacy of the Estrogen Patient Package Insert
Authors: Gail P. Udkow
Journal: JAMA (1979)

IN MARCH 1975, several consumer organizations petitioned the Food and Drug Administration, requesting that written information containing pertinent warnings be included with certain prescription drugs. Following solicitation of comments from interested persons (American Medical Association and consumer groups), the FDA issued a regulation,<sup>1</sup>effective October 1977, requiring distribution of a patient package insert (PPI) with all estrogenic drug products. Excluded were estrogen-containing oral contraceptives, for which a PPI has existed since 1970, and diethylstilbestrol products intended for postcoital contraception. According to J. Richard Crout, MD, director of the Bureau of Drugs of the FDA, "These [estrogen agents] were chosen because they are used by normal women as contraceptive and postmenopausal agents for which reasonable alternatives exist."<sup>2</sup>The current PPI must additionally be distributed to all women receiving postpartum estrogen therapy for suppression of lactation. Few studies have evaluated patients' interest in PPIs. Joubert and Lasagna<sup>3</sup>assessed the
Abstract 17191: Bolus Administration T1 Mapping as a Marker of Interstitial Myocardial Fibrosis in Severe Aortic Stenosis
Authors: Andrew Jabbour, Callum Ettles, Tevfik Ismail, Ankur Gulati, Pedro Ferreira, Ricardo Wage, Saman Zaman, Pierre Croisille, Carl Shakespeare, Raad Mohiaddin, Taigang He, Dudley Pennell, David Firmin, Sanjay Prasad
Journal: Circulation (2011)

Introduction: Aortic stenosis (AS) is associated with increased interstitial myocardial fibrosis (IMF). This is detectable by cardiovascular magnetic resonance (CMR) after gadolinium infusion. T1-mapping-derived partition coefficients (λ) and extracellular volume fractions (Ve) have also been shown to correlate with IMF after a simple bolus administration of gadolinium. We hypothesized that the λ and Ve would also detect interstitial expansion in severe AS patients compared to healthy controls and that these measures would correlate with abnormal myocardial strain using a high temporal-resolution tagging sequence. Methods: A Modified Look-Locker Inversion Recovery (MOLLI) sequence was used to generate eleven T1-weighted images. Myocardial and blood pool T1 values were derived by fitting a signal intensity-time curve using CMR42®. The λ was determined by plotting (1/T1 myo vs. 1/T1 blood pool ) at various time points once contrast equilibrium was reached. Ve was derived accounting for the hematocrit. Short-axis T1 maps were acquired using a 1.5T scanner (Siemens) before and 1,2,5,8,15,20,25 and 30 minutes after contrast. Myocardial tagging images were acquired using single- and multiple-breath-hold CSPAMM sequences in multiple planes and analysed with inTag® (Lyon, France). Results: Twenty-three subjects (age 57 (16), mean (SD); male n=13; severe AS n= 8, healthy control n=15) were recruited. Subjects with severe AS displayed higher λ (0.35 (0.11) vs 0.27 (0.04); P=0.02) and Ve (0.19 (0.08) vs 0.13 (0.03)).The λ and Ve correlated with indices of reduced myocardial function including reduced circumferential δ strain (r=-0.69, p=0.001), angle peak strain (r=-0.48, p=0.04) and radial δ strain (r=-0.59, p=0.03); and increased left atrial dilatation (r=0.64, p=0.001). Conclusions: T1-mapping-derived λ and Ve are significantly elevated in patients with AS compared to healthy controls and correlate well with indices of reduced myocardial performance. This difference was quantifiable after a simple bolus administration of gadolinium. Further histological validation is required; however, T1-mapping λ and Ve derivation after bolus gadolinium administration is clinically practical and holds promise for the detection of IMF in severe AS.
NEED FOR RESEARCH IN THE TREATMENT OF EPIDERMOPHYTOSES
Authors: JAMES HERBERT MITCHELL
Journal: JAMA (1927)

Cultural studies of the pathogenic fungi have offered so much of interest and promise that other phases of the epidermophytoses have been relatively neglected. I do not minimize the importance of determinative mycology, nor would I suggest that it give way to other investigation; but it is evident to many dermatologists that our present methods of treatment are far from satisfactory. During the past decade widespread interest in the fungous infections has been stimulated by an ever increasing number of papers on the subject from all parts of the civilized world, and by an ever increasing number of clinical cases, both in public and in private practice. Very little attention, however, has been devoted to the purely therapeutic side of the question. It is a noteworthy fact that the first intensive laboratory attack on the problem was made in this country by Schamberg and Kolmer,<sup>1</sup>who determined the fungicidal
Population analysis of cellular responses to synthetic peptides of <i>Der p</i> II, a major allerg molecule of <i>Dermatophagoides pteronyssinus</i>, in allergic and nonallergic subjects
Authors: M. Okano, T. Nagano, K. Kino, H. Yasueda, Y. Baba, C. Saito, Y. Masuda, N. Ohta
Journal: Allergy (1994)

Okano M, Nagano T, Kino K, Yasueda H, Baba Y, Saito C, Masuda Y, Ohta N. Population analysis of cellular responses to synthetic peptides of Der p II, a major allergen molecule of Dermatophagoides pteronyssinus , in allergic and nonallergic subjects. Responses of peripheral blood mononuclear cells to synthetic oligopeptides of Der p II, one of the major allergen molecules of Dermatophagoides pteronyssinus , were compared between allergic and nonallergic subjects. Healthy subjects showed positive responses to crude extracts of D. pteronyssinus , but only allergic subjects showed elevated cellular responses to Der p II. We synthesized three oligopeptides of Der p II in which motifs of a possible T‐cell epitopc were included. Of 14 subjects with positive response to Der p II, three responded to all three peptides, while five did not respond to any peptide tested. In 11 allergic patients who showed positive response to Der p II, responsiveness to the peptide K33‐T47 was significantly higher than that to other peptides ( P &lt;0.05). All the responding patients were also positive for scratch test to Der p II, suggesting that those epitopcs induced IgE‐promoting helper T‐cell response in allergic persons. On the other hand, the in vitro cellular responses were not necessarily correlated to IgE production against Der p II in healthy subjects.
HEMODYNAMIC EFFECTS OF 1-HYDRAZINOPHTHALAZINE (APRESOLINE) IN HUMAN PREGNANCY: RESULTS OF INTRAVENOUS ADMINISTRATION 1
Authors: N. S. Assali, S. Kaplan, S. Oighenstein, R. Suyemoto
Journal: Journal of Clinical Investigation (1953)

Gross, Druey and Meier (1) first demonstrated that 1-hydrazinophthalazine (Apresoline) 8 given intravenously or orally to experimental animals, produces a marked and prolonged hypotensive ef- fect, with simultaneous increases in pulse rate, auricular pressure, and renal blood flow.Further animal experiments showed that this drug is effective against both neurogenic and renal hypertension (2, 3, 4), and that its hemodynamic effects are different from those of typical auto- nomic or adrenergic blocking agents (1, 5).Marked inhibition and even reversal of the vaso- pressor effects of epinephrine by Apresoline have been observed by some investigators (1, 2, 6, 7), whereas others, despite the use of large doses of the latter drug, have obtained only slight if any inhibition of such effects (8, 9).Apresoline does not seem to alter the pressor response to hypoxia or to the carotid sinus reflex (6, 9).It has been suggested that it may act through the hypothala- mus or that it may counteract the effects of cir- culating vasopressor substances (1, 3, 4, 10).The drug increases cardiac output and renal blood flow in unanesthetized animals, whereas renal blood flow remains unaltered in anesthetized animals (7).Patients with hypertension of diverse etiology seem to respond to the vasodepressor action of Apresoline, even when hypertension has persisted or recurred after sympathectomy (3, 4, 10).The induced fall in arterial blood pressure is usually
Review: Low-dose corticosteroids improve outcomes in acute lung injury and the acute respiratory distress syndrome
Authors: Niall D. Ferguson
Journal: Annals of Internal Medicine (2009)

Source Citation Tang BM, Craig JC, Eslick GD, Seppelt I, McLean AS. Use of corticosteroids in acute lung injury and acute respiratory distress syndrome: a systematic review and meta-analysis. Crit Care Med. 2009;37:1594-603. 19325471
Pulmonary Edema During Fluid Infusion in the Absence of Heart Failure
Authors: Leon Stein
Journal: JAMA (1974)

Six critically ill patients with clinical and hemodynamic signs of hypovolemia developed roentgenographic evidence of pulmonary edema (PE) without cardiac enlargement after infusion of an average of 6.4 liters of crystalloids. Left ventricular filling pressures remained normal. However, colloid osmotic pressure (COP) decreased to 15 torr, substantially lower than the normal of 25 torr. The low COP may account for the development of PE in the absence of pulmonary hypertension occurring singly or concurrently with changes in interstitial pressure or capillary permeability. (<i>JAMA</i>229:65-68, 1974)
THE RESPONSE TO RESPIRATORY RESISTANCE
Authors: Richmond L. Moore, Carl A. L. Binger
Journal: The Journal of Experimental Medicine (1927)

1. A study has been made of the effects of resistance to respiration in the inspiratory and expiratory phases. 2. Resistance to inspiration caused an increase in respiratory rate, a decrease in tidal air, and in most instances a severe limitation of the minute volume of pulmonary ventilation. Anoxemia and acidosis accompanied these changes. 3. When resistance was removed the respiratory rate continued to be rapid, but the tidal air and minute volume increased. As a result of this there was a fall in pCO(2), a rise in pH, and in some cases a complete disappearance of anoxemia. 3. Resistance to expiration slowed the respiratory rate and produced a constant decrease in the minute volume of pulmonary ventilation. Anoxemia and carbon dioxide retention occurred, but were less pronounced than in the inspiratory experiments. Release of resistance to expiration resulted in a return of all functions to their normal, or approximately normal, levels. 4. A difference in the gross pulmonary pathology found at autopsy in these two types of experiments has been described, and an attempt has been made to correlate changes in function with changes in structure. 5. No direct evidence has been supplied for the liability to fatigue of the respiratory center.
ABSCESS OF FIBROID UTERUS PERFORATING INTO GENERAL PERITONEAL CAVITY
Authors: Herbert E. Stein
Journal: JAMA (1923)

The danger of fibroid tumors of the uterus lies not so much in their presence per se, as in the secondary changes that they may undergo, their remote effects on the cardiovascular system, and their pressure on neighboring organs and tissues. Of 2,274 cases of uterine fibroids reported by Noble, these degenerative changes and complications were noted: necrosis of tumor; myxomatous degeneration; hyaline degeneration; cystic degeneration; fatty degeneration; calcareous infiltration; malignancy; edema; twisted pedicle; uncontrollable hemorrhage, and aberrant sites. In this large series, suppuration was absent, although its presence has been noted by other authors. However, its infrequency is apparent. In a fairly extensive review of the literature, I have failed to come across the report of an abscess of a fibroid perforating into the general peritoneal cavity. A report of the case seems worth while. <h3>REPORT OF CASE</h3> Mrs. S., aged 44, admitted to the gynecologic service of Harlem