tbuckley/cabot-search huggingface dataset:
Abstract A79: Elucidating the role of E2F2 transcription factor in mediating human breast cancer metastasis
Authors: Inez Yuwanita, Danielle Barnes, Eran Andrechek
Journal: Cancer Research (2013)

Abstract In human breast cancer metastasis, it is not the primary tumor that is the cause of mortality but metastasis to distant sites. Therefore, it is important to elucidate the biological mechanism that underlies the metastatic processes. To address this, we utilized bioinformatic methods in combination with biochemical assays and genetic studies. Specifically, we crossed a MMTV-Myc mouse model with various activator E2Fs mutants and found that the tumor incidence decreased in the MMTV-Myc mice crossed with E2F2 and E2F3 mutant background. Surprisingly, we also found that the loss of E2F2 sharply increased lung metastasis in MMTV-Myc mice. Here, we test whether these observations in mice translate to human breast cancer and identify the genes under the control of E2F2 transcription factor that are responsible for mediating breast cancer metastasis. To achieve these aims, we examined the probability of activation of the E2F2 transcription factors in human breast cancer. We calculated the probability of E2F2 activation and used this data to stratify the human breast cancer samples and their associated metastatic clinical data. We performed hierarchical clustering of human and mouse gene expression datasets and uncovered two clusters of human breast tumors in which E2F2 played distinctly different roles in mediating metastasis. The human breast cancer samples that clustered with our mouse gene expression data exhibited the same role for E2F2 whereby it increased time to distant metastasis. We then examined genes that were differentially regulated within this cluster and found that there was a set of genes that were associated with a difference in human metastasis survival times and with the E2F2 knockout in the mouse model. To further explore the mechanism by which E2F2 mediates human breast cancer metastasis, we have identified four genes asputative E2F2 target genes including KLK1, MYH2, PTPRD and TNNC2. We found that partial knockdown of KLK1 promotes the migratory capacity of MCF7 cell in vitro. In addition, partial knockdown of PTPRD affected cellular morphology, where they were found to grow in discreet clusters while having little effect on the migration capacity in a scratch assay. We are currently exploring the effects of MYH2 and TNNC2 ablation on these cells. Taken together, we have begun to elucidate the roles of E2F2 transcription factor in mediating breast cancer metastasis as well as establishing a mechanism by which E2F2 regulates metastasis. Citation Format: Inez Yuwanita, Danielle Barnes, Eran Andrechek. Elucidating the role of E2F2 transcription factor in mediating human breast cancer metastasis. [abstract]. In: Proceedings of the AACR Special Conference on Tumor Invasion and Metastasis; Jan 20-23, 2013; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2013;73(3 Suppl):Abstract nr A79.
US pacemaker manufacturer faces British lawsuit
Authors: O. Dyer
Journal: BMJ (2003)

St Jude Medical, a major manufacturer of heart valves and pacemakers, is to face a lawsuit in Britain over its Silzone heart valves, which were discontinued after a recall in January 2000.

The Minnesota based company, which is already the target of class action lawsuits over Silzone in the United States and Canada, is to be sued in London's High Court by Billy McCombe of Northern Ireland. Mr McCombe's wife, Myrtle, died in July 1998, aged 44, 10 weeks after receiving a Silzone mitral valve implant.

The Silzone valve is a variant of the standard St Jude valve, with a …
An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid
Authors: Mathias Chamaillard, Masahito Hashimoto, Yasuo Horie, Junya Masumoto, Su Qiu, Lisa Saab, Yasunori Ogura, Akiko Kawasaki, Koichi Fukase, Shoichi Kusumoto, Miguel A Valvano, Simon J Foster, Tak W Mak, Gabriel Nuñez, Naohiro Inohara
Journal: Nature Immunology (2003)
Should C Be a Passing Grade for the USPSTF?
Authors: Barry G. Saver
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Runx1 Expression Marks Long-Term Repopulating Hematopoietic Stem Cells in the Midgestation Mouse Embryo
Authors: Trista E North, Marella F.T.R de Bruijn, Terryl Stacy, Laleh Talebian, Evan Lind, Catherine Robin, Michael Binder, Elaine Dzierzak, Nancy A Speck
Journal: Immunity (2002)
SENSITIVITY TO HYDROCORTISONE ACETATE OINTMENT
Authors: RONALD CHURCH
Journal: British Journal of Dermatology (1960)

Journal Article SENSITIVITY TO HYDROCORTISONE ACETATE OINTMENT Get access RONALD CHURCH, M.A., M.D., M.R.C.P.E. RONALD CHURCH, M.A., M.D., M.R.C.P.E. The Rupert Hallam Department of Dermatology, The Royal Infirmary, Sheffield Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 72, Issue 10, 1 October 1960, Pages 341–344, https://doi.org/10.1111/j.1365-2133.1960.tb13814.x Published: 01 October 1960
Risk factors and impact of major bleeding in critically ill patients receiving heparin thromboprophylaxis
Authors: François Lauzier, Donald M. Arnold, Christian Rabbat, Diane Heels-Ansdell, Ryan Zarychanski, Peter Dodek, Betty Jean Ashley, Martin Albert, Kosar Khwaja, Marlies Ostermann, Yoanna Skrobik, Robert Fowler, Lauralyn McIntyre, Joseph L. Nates, Tim Karachi, Renato D. Lopes, Nicole Zytaruk, Simon Finfer, Mark Crowther, Deborah Cook
Journal: Intensive Care Medicine (2013)
Relationship of blood pressure in 20-39-year-old men to subsequent blood pressure and incidence of hypertension over a 30-year observation period.
Authors: S W Rabkin, F A Mathewson, R B Tate
Journal: Circulation (1982)

The objective of this investigation was to determine the relationship of blood pressure (BP) in young men, ages 20-39 years, to their subsequent BP from the perspective of BP tracking, position in BP distribution and later evidence of hypertensive BP values. Since 1948, the Manitoba Study group has followed 3983 men, 90% of whom were 20-39 years old at entry. BP in persons not prescribed antihypertensive medications was examined at 5-year intervals during the 30-year observation period to 1978. To adjust for age, BP was examined within 5-year age groups at entry. The correlations between entry and subsequent BP at the same length of follow-up were greater for systolic than diastolic BP and increased with older ages. The correlation decreased wtih every 5-year examination after entry for all ages. Men whose BP was below the mean at entry were less likely to have a BP greater than 1 standard deviation (SD) above the mean at any of the examinations. Men with an entry BP greater than 1 SD above the mean were more likely to have BP greater than 1 SD above the mean later, but the relationship decreased considerably after 20 years, especially in 20-24-year age group. The results were similar for the probability of hypertension values (systolic BP greater than or equal to 140 or 150 mm Hg, diastolic BP greater than or equal to 90 or 95 mm Hg) at later examinations. Thus, BP in later life can be predicted from BP at ages 20-39 years and can identify groups at high or low risk for hypertension.
Selective Bronchial Catheterization
Authors: Walter H. Thiede, Edward F. Banaszak
Journal: New England Journal of Medicine (1972)

THE technic of selective bronchial catheterization and brushing has been described by authors in a number of medical centers.1 2 3 4 The procedure was popularized by Fennessy,1 who used preformed catheters to attain placement of the catheter in a selected area. Fennessy1 , 2 has chosen a nasotracheal approach, whereas oratracheal4 and transcricoid approaches3 have also been used. Bronchial brushing has been described4 as the most accurate diagnostic tool short of thoracotomy in the diagnosis of lung cancer. It is especially valuable since it allows access to peripheral lesions.This procedure should be considered for any hospital that is provided with an image intensifier . . .
Response to: ‘Concerns about report suggesting glucosamine and chondroitin protect against cartilage loss’ by Felson
Authors: Johanne Martel-Pelletier, Camille Roubille, François Abram, Marc Hochberg, Marc Dorais, Philippe Delorme, Jean-Pierre Raynauld, Jean-Pierre Pelletier
Journal: Annals of the Rheumatic Diseases (2015)

We wish to address the comments by Felson1 regarding our recently published article . 2

This study used the osteoarthritis initiative (OAI) cohort database, which offers a unique opportunity to explore the onset and progression of knee osteoarthritis (OA) and the potential effects of treatments on the natural progression of the disease. Obviously, as does any observational study, such cohort has limitations. The rationale behind the referenced work was to build a study design that circumvented some of the limitations of the OAI cohort by selecting a subpopulation of the participants. We wanted to study, as reliably as possible, the potential disease-modifying osteoarthritis drug (DMOAD) effects of glucosamine (Glu) and chondroitin sulfate (CS) in a design that would mimic a randomised controlled trial (RCT) with all of the restrictions that such a study design could impose in the context of an observational study. From the first-line analysis it was obvious that the patient population was heterogeneous from a clinical standpoint, with one of the main factors being related to disease treatment (±analgesics/non-steroidal anti-inflammatory drugs (NSAIDs)) and the implicit severity of the disease based on symptoms and joint space width (JSW). This observation was interesting and, importantly, clinically relevant.

As outlined in the study design section, we …
Touching on the Keys to Visual Memory
Authors: Neil D. Ravin
Journal: JAMA (1991)

<h3>To the Editor.</h3> —I have made a clinical observation about memory, which I cannot explain but invite others to try. For 30 years, I have taken notes on my patients using a typewriter and the "touch-typing" system without looking at the keyboard or losing eye contact with my patients. Today, I tried to draw a picture of the keyboard from memory but could not go beyond figuring out a few of the home keys. An informal survey of secretaries at the hospital who touch type revealed that when their keyboards were covered, none was any better than I. After looking at the keyboard and studying it briefly, I was able to reconstruct it, but was unable to visualize the board with which I was so familiar without "re-encoding." How can this inability to visually construct the keyboard be squared with the functional facility that allows me to use it? Is
Rapid Single-Step Induction of Functional Neurons from Human Pluripotent Stem Cells
Authors: Yingsha Zhang, ChangHui Pak, Yan Han, Henrik Ahlenius, Zhenjie Zhang, Soham Chanda, Samuele Marro, Christopher Patzke, Claudio Acuna, Jason Covy, Wei Xu, Nan Yang, Tamas Danko, Lu Chen, Marius Wernig, Thomas C. Südhof
Journal: Neuron (2013)
On-Pump and Off-Pump Bypass Surgery
Authors: Bruce W. Lytle, Joseph F. Sabik
Journal: Circulation (2004)

ardiopulmonary bypass (CPB) has been fundamental to the development of coronary bypass surgery.Although some early coronary operations were performed without CPB, widespread coronary surgery only occurred with the aid of perfusion, a support system that has allowed complex coronary reconstruction to be carried out with effective myocardial protection, end organ protection, and consistent and relative safety.
Twelve Brown Eggs
Authors: Jack L. Mayer
Journal: JAMA (1987)

Twelve brown eggs. Every Wednesday they're on my desk like a dozen inflated pennies. Mrs Labelle leaves them as payment on her bill in my pediatrics office on the Canadian border. She has little money but lots of chickens. In the northeast kingdom of Vermont the "economic recovery" doesn't mean much in terms of cold cash. When I first came to this town ten years ago, filled with the romance of rural New England, I posted a sign inviting barter: "Times are tough. If you are having trouble paying your bill, I would be happy to discuss bartering goods or services." It wasn't long before Mrs Boisonneau began crocheting a series of blankets, hats, and sweaters that far exceeded the number of beds, heads, and bodies in my family. Anthony and Edward Delain seemed to pick up every croup, otitis, and bronchitis plaguing the county, but Mr Delain, an independent
STUDIES ON THE RELATIONSHIP OF PTEROYLGLUTAMIC ACID TO THE GROWTH OF PSITTACOSIS VIRUS (STRAIN 6BC)
Authors: Herbert R. Morgan
Journal: The Journal of Experimental Medicine (1948)

p-Aminobenzoic and pteroic acids antagonize the inhibition of the growth of psittacosis virus (strain 6BC) by sulfadiazine in a competitive manner. Pteroylglutamic acid exerts a non-competitive type sulfonamide antagonism. The effects of certain analogues of pteroylglutamic acid and of pteroic acid on the growth of psittacosis virus and its inhibition by sulfadiazine are reported. The implications of these findings with regard to the possible synthesis of pteroylglutamic acid by psittacosis virus and its suggested rôle as an essential factor for growth of this virus are discussed.
P260Quantification of systemic cardiac troponin overestimates infarct size in presence of left ventricular hypertrophy: insights into the underlying mechanisms
Authors: R Fernandez-Jimenez, S Martinez-Martinez, D Lopez-Maderuelo, J Silva, M Nuno-Ayala, G Javier-Lopez, J Garcia-Prieto, V Fuster, JM Redondo, B Ibanez
Journal: Cardiovascular Research (2014)

Introduction and Purpose: Myocardial infarct size estimation using biomarkers -creatine kinase (CK) and troponin (cTn) - is commonly used as the primary endpoint in many experimental studies and clinical trials. We recently described for the first time in a retrospective study that peak of cTnI could overestimate infarct size in presence of left ventricular hypertrophy (LVH). Here we sought to decipher the mechanism underlying this clinical observation. Methods: Pigs (n=10) with previously induced LVH by aortic banding and sham (non-LVH) control pigs (n=8) underwent myocardial infarction. Cardiac magnetic resonance (CMR) was performed 7 days after infarction, and CK and cTnI systemic values were serially measured. Histological sections from LVH and sham pigs were analyzed to detect cTnI expression by immunofluorescence confocal microscopy. Fluorescence intensity and size in cardiomyocytes were quantified, and the comparison of data was performed using the two-tailed Student's t-test. The clinical prospective validation was done by evaluating CMR from 140 patients with anterior STEMI to quantify LVH and infarct size correlating these parameters with release of damage biomarkers by multiple lineal regression analyses. Results: Both in the experimental and clinical studies, LVH was associated with a significant increase in peak and area under the curve (AUC) of cTnI. These results were observed either when LVH was categorized or when indexed LV mass was evaluated as a continuous variable (p<0.05 for all analyses). No significant differences were found in peak/AUC of total CK between LVH vs. no-LVH neither in the experimental nor the clinical study. Analysis of the fluorescence intensity revealed a significant increase in the content of cTnI in hypertrophic cardiomyocytes compared to regular cardiomyocytes derived from LVH and non-LVH pigs, respectively (p<0.05). Conclusions: Quantification of released cardiac troponin-I overestimates infarct size in presence of LVH due to an increase in its content in the hypertrophic cardiomyocyte. Left ventricular hypertrophy should be taken into consideration when infarct size is estimated by troponin release.
Correction of Anemia with Epoetin Alfa in Chronic Kidney Disease
Authors: Ajay K. Singh, Lynda Szczech, Kezhen L. Tang, Huiman Barnhart, Shelly Sapp, Marsha Wolfson, Donal Reddan
Journal: New England Journal of Medicine (2006)

Anemia, a common complication of chronic kidney disease, usually develops as a consequence of erythropoietin deficiency. Recombinant human erythropoietin (epoetin alfa) is indicated for the correction of anemia associated with this condition. However, the optimal level of hemoglobin correction is not defined.
Australia changes confidentiality code
Authors: C. Zinn
Journal: BMJ (1996)

The Australian Medical Association (AMA) is under attack from AIDS groups and civil libertarians for making changes to the general practice code of ethics, which will mean that doctors may breach patient confidentiality (see also p78). The radical new clause, to take effect from February, has formalised the circumstances in which doctors may compromise their relationship with patients when the “health of others is at risk.”

The association's new code of ethics says: “Keep in confidence information derived from your patient, or from a colleague regarding your patient, and divulge it only with the patient's permission. Exceptions may arise where the health of others is at …
Abstract 5172: Role of CEACAM1 on cancer cell endothelial adhesion and liver metastasis in vivo
Authors: Carlos H.F. Chan, Azadeh Arabzadeh, Luisa DeMarte, Jonathan Spicer, Claire Turbide, Pnina Brodt, Nicole Beauchemin, Lorenzo E. Ferri
Journal: Cancer Research (2010)

Abstract Background: There are emerging data to suggest that tumor over-expression of the carcinoembryonic antigen-related cell adhesion molecules (CEACAM1, 5 and 6) may influence cancer metastasis; however, the mechanisms for this are unclear. CEACAM1 was shown to bind homotypically to itself and heterotypically to CEACAM5 and CEACAM6. Since CEACAM1 is the only known CEACAM family member expressed on the hepatic endothelium, we hypothesized that CEACAM1 promotes cancer metastasis by directly mediating binding between circulating tumor cells and the sinusoidal endothelium. Methods: Using intra-vital microscopy (IVM), a physiologically relevant model system to assess the early stages of liver metastasis in vivo, we have compared the hepatic endothelial adhesion of intra-splenically injected MC38 cells (mouse colon cancer cell line with minimal CEACAM1 expression) between Ceacam1+/+ and Ceacam1−/− mice. A CEACAM1-negative population of MC38 cells (MC38-null) was sorted out by FACS using specific anti-CEACAM1 antibodies. MC38-null cells were then infected with retroviruses produced from ψ2 packaging cells carrying pLXSN-CEACAM1 expression construct and infected cells were selected using neomycin-containing media. A CEACAM1-positive population of MC38 cells (MC38-CC1) was sorted out by FACS using specific anti-CEACAM1 antibodies. The migratory ability of MC38-null and MC38-CC1 cells were compared by IVM in Ceacam1+/+ mice as mentioned above. Liver metastasis assays were performed by means of intra-splenic injection of MC38 cells into the Ceacam1+/+ and Ceacam1−/− mice. Data expressed as mean ± SEM, student t-test determined significance (*p&amp;lt;0.05). Results: By using IVM, we observed a 3-fold decrease in hepatic sinusoidal endothelial adhesion of intra-splenically injected MC38-null cells in Ceacam1−/− mice compared to Ceacam1 +/+ mice (0.8±0.2 vs 2.5±0.3 adhered cells)*. Forced expression of CEACAM1 in MC38 cells however did not significantly increase hepatic endothelial adhesion in the Ceacam1+/+ mice (2.7±0.9 adhered MC38-CC1 cells vs 2.5±0.2 adhered MC38-null cells). Development of liver metastasis after intra-splenic injection of MC38-null cells was significantly reduced in the Ceacam1−/− mice (81% reduction in comparison to Ceacam1+/+ mice)*. Conclusion: Sinusoidal endothelial cell, but not cancer cell, CEACAM1 expression supports liver metastasis by increasing the adherence of circulating tumor cells to hepatic endothelium. Further investigation will be required to dissect the exact mechanism of how endothelial CEACAM1 influence hepatic endothelial adhesion of cancer cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5172.
Alteration of a Single Hydrogen Bond between Class II Molecules and Peptide Results in Rapid Degradation of Class II Molecules after Invariant Chain Removal
Authors: Stephanie Ceman, Shenhong Wu, Theodore S. Jardetzky, Andrea J. Sant
Journal: The Journal of Experimental Medicine (1998)

To characterize the importance of a highly conserved region of the class II β chain, we introduced an amino acid substitution that is predicted to eliminate a hydrogen bond formed between the class II molecule and peptide. We expressed the mutated β chain with a wild-type α chain in a murine L cell by gene transfection. The mutant class II molecule (81βH−) assembles normally in the endoplasmic reticulum and transits the Golgi complex. When invariant chain (Ii) is coexpressed with 81βH−, the class II–Ii complex is degraded in the endosomes. Expression of 81βH− in the absence of Ii results in a cell surface expressed molecule that is susceptible to proteolysis, a condition reversed by incubation with a peptide known to associate with 81βH−. We propose that 81βH− is protease sensitive because it is unable to productively associate with most peptides, including classII–associated invariant chain peptides. This model is supported by our data demonstrating protease sensitivity of peptide-free wild-type I-Ad molecules. Collectively, our results suggest both that the hydrogen bonds formed between the class II molecule and peptide are important for the integrity and stability of the complex, and that empty class II molecules are protease sensitive and degraded in endosomes. One function of DM may be to insure continuous groove occupancy of the class II molecule.