tbuckley/cabot-search huggingface dataset:
The Sea Urchin sns5 Insulator Protects Retroviral Vectors From Chromosomal Position Effects by Maintaining Active Chromatin Structure
Authors: Danilo D'Apolito, Elena Baiamonte, Mariella Bagliesi, Rosalba Di Marzo, Roberta Calzolari, Leda Ferro, Vito Franco, Giovanni Spinelli, Aurelio Maggio, Santina Acuto
Journal: Molecular Therapy (2009)

Silencing and position-effect (PE) variegation (PEV), which is due to integration of viral vectors in heterochromatin regions, are considered significant obstacles to obtaining a consistent level of transgene expression in gene therapy. The inclusion of chromatin insulators into vectors has been proposed to counteract this position-dependent variegation of transgene expression. Here, we show that the sea urchin chromatin insulator, sns5, protects a recombinant gamma-retroviral vector from the negative influence of chromatin in erythroid milieu. This element increases the probability of vector expression at different chromosomal integration sites, which reduces both silencing and PEV. By chromatin immunoprecipitation (ChIP) analysis, we demonstrated the specific binding of GATA1 and OCT1 transcription factors and the enrichment of hyperacetylated nucleosomes to sns5 sequences. The results suggest that this new insulator is able to maintain a euchromatin state inside the provirus locus with mechanisms that are common to other characterized insulators. On the basis of its ability to function as barrier element in erythroid milieu and to bind the erythroid specific factor GATA1, the inclusion of sns5 insulator in viral vectors may be of practical benefit in gene transfer applications and, in particular, for gene therapy of erythroid disorders.
Potential Health and Economic Consequences of Misplaced Priorities
Authors: Steven H. Woolf
Journal: JAMA (2007)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
<i>In vitro</i>activity of the lipopeptide derivative (Pal-lys-lys-NH<sub>2</sub>), alone and in combination with antifungal agents, against clinical isolates of dermatophytes
Authors: O. Simonetti, D. Arzeni, G. Ganzetti, C. Silvestri, O. Cirioni, E. Gabrielli, S. Castelletti, W. Kamysz, E. Kamysz, G. Scalise, A. Offidani, F. Barchiesi
Journal: British Journal of Dermatology (2009)

An increasing number of antimycotics have become available for the treatment of dermatophytoses; however, there are reports suggesting recalcitrance to therapy or resistance of a dermatophyte against conventional treatment. Lipopeptides represent novel therapeutic drugs with a new mode of action.The aim of this study was to investigate the in vitro effects of the lipopeptide Pal-Lys-Lys-NH(2) (PAL) alone and in combination with standard antifungal agents, such as fluconazole (FLU), itraconazole (ITRA) and terbinafine (TER) against 24 clinical isolates of dermatophytes belonging to four species.A broth microdilution method following the Clinical and Laboratory Standards Institute recommendations (M38-A) was used for testing drugs alone and in combination.PAL minimum inhibitory concentrations (MICs) ranged from < or = 0.25 to > 16 microg mL(-1) and they were similar to those of FLU and higher than those of either ITRA or TER. Synergy, defined as a fractional inhibitory concentration (FIC) index of < or = 0.50, was observed in 67%, 52% and 15% of PAL/ITRA, PAL/TER and PAL/FLU interactions, respectively. None of these combinations yielded antagonistic interactions (FIC index > 4). When synergy was not achieved, there was still a decrease in the MIC of one or both drugs used in the combination.Our study demonstrates that PAL has potential activity against dermatophytes. In addition, the in vitro activity of PAL can be enhanced upon combination with standard drugs. This lipopeptide applied in the form of lacquer, spray or ointment, could represent an interesting new therapy, particularly when combined with conventional treatment in recalcitrant or resistant dermatophyte infections.
Rheumatic Fever: Its Dangers and its Treatment
Authors: J. Roberton
Journal: BMJ (1864)

ModicalJouruaLthe arachnoid cavity in fourteen; and in all these, with the exception of one, consciousness was cornpletely abolished.Again, of the fourteen cases, there was no paralysis in nine, and of these the hiemorrhage was entirely confined to the arachnoid in four; in five, there existed partial paralysis, but there was lesion in other parts, besides the arachnoid, in all ex- cept one.In most of the cases the coma continued until death-an event which very rapidlysupervened.Oc- casionally, however, the patients lapsed from a state of complete coma into that of partial unconsciousness.In others, again, consciousness was completely re-esta- blished; but in these cases the memory was feeble,
NUP98 rearrangements in hematopoietic malignancies: a study of the Groupe Francophone de Cytogénétique Hématologique
Authors: S P Romana, I Radford-Weiss, R Ben Abdelali, C Schluth, A Petit, N Dastugue, P Talmant, C Bilhou-Nabera, F Mugneret, M Lafage-Pochitaloff, M-J Mozziconacci, J Andrieu, J-L Lai, C Terre, K Rack, P Cornillet-Lefebvre, I Luquet, N Nadal, F Nguyen-Khac, C Perot, J Van den Akker, S Fert-Ferrer, C Cabrol, C Charrin, I Tigaud, H Poirel, M Vekemans, O A Bernard, R Berger
Journal: Leukemia (2006)
Psychiatry's Drift Away from Medicine
Authors: RICHARD A. SCHWARTZ
Journal: American Journal of Psychiatry (1974)

The author discusses a number of trends in current psychiatric theory and practice that he believes have tended to separate psychiatry from its traditional medical orientation. These include an overly broad concept of psychiatric illness, overinvolvement by psychiatrists in nonmedical service programs, delegation of diagnosis and treatment to nonmedical personnel, and physically separate facilities for psychiatric and general medical services. Believing that these trends are having adverse consequences, he makes some specific suggestions that will enable psychiatry to resume closer ties with the rest of medicine.
Cish attenuates proximal TCR-signaling and CD8+ T cell immunity
Authors: Douglas Palmer, Geoffery Guittard, Shashank Patel, Lawrence E Samelson, Nicholas P Restifo
Journal: Journal for ImmunoTherapy of Cancer (2014)

Meeting abstracts CD8+ T cells are potent killers of infected cells and tumors, specifically recognizing their targets through the T cell receptor (TCR). While much has been revealed about the activation of TCR signaling, negative regulation of this pathway remains incompletely elucidated. We
Identification of truncated RUNX1 and RUNX1-PRDM16 fusion transcripts in a case of t(1;21)(p36;q22)-positive therapy-related AML
Authors: M-J P L Stevens-Kroef, E F P M Schoenmakers, M van Kraaij, E Huys, S Vermeulen, B van der Reijden, A Geurts van Kessel
Journal: Leukemia (2006)
Isotope Scanning for Diagnosing Lesions Projecting Into the Lower Chest
Authors: Giomar Gonzalez
Journal: JAMA (1971)

Intra-abdominal masses, particularly those arising from the liver and spleen, may project into the lower part of the chest and thus necessitate an extensive diagnostic work-up. The use of liver and spleen isotope scans early in the evaluation of such cases will frequently provide the correct diagnosis and save significant time and cost.
Ecological Uptake and Depuration of Carbon Nanotubes by <i>Lumbriculus variegatus</i>
Authors: Elijah J. Petersen, Qingguo Huang, Walter J. Weber
Journal: Environmental Health Perspectives (2008)

Carbon nanotubes represent a class of nanomaterials having broad application potentials and documented cellular uptake and ecotoxicological effects that raise the possibility that they may bioaccumulate in living organisms.Radioactively labeled nanotubes were synthesized using a novel methane chemical vapor deposition procedure. Single-walled carbon nanotubes (SWNTs), multiwalled carbon nanotubes (MWNTs), and pyrene were spiked to sediment samples, and the respective uptake and depuration of these nanotubes and pyrene were assessed by the oligochaete, Lumbriculus variegatus.(14)C-labeled carbon nanotubes were developed for these experiments to overcome significant previous limitations for quantifying nanotube materials in environmental and biological media. Biota-sediment accumulation factors for SWNTs and MWNTs were observed to be almost an order of magnitude lower than those for pyrene, a four-ringed polycyclic aromatic hydrocarbon (PAH). The depuration behaviors of the oligochaete suggested that the nanotubes detected in these organisms were associated with sediments remaining in the organism guts and not absorbed into cellular tissues as was the pyrene. The results suggest that, unlike PAHs, purified carbon nanotubes do not readily absorb into organism tissues.
HARE-LIP AND CLEFT PALATE: A WAR INFLUENCE
Authors: J. L. Aymard
Journal: BMJ (1921)

M ITJOURNAL 405 left maxillary antra, both ethmoids, both frontal sinuses, and one sphenoidal sinus.There was also a discharging opening at the inner angle of the right orbit which comrn mnunicated with the frontal sinus.<The teeth were removed, the antra opened, and the ethmoids curetted.Each frontal sinus was ionized twice, and an autogenous vaccine given.The sphenoidal sinus was ionized and suppuration in it ceased at once.The -opening into the orbit closed without any further treatment, and in a slhort time all discharge from the nose ceased and did not recur.
NHS Direct will close down in March next year
Authors: I. Torjesen
Journal: BMJ (2013)

England’s NHS Direct service has announced that it will close completely by the end of this financial year and that all its remaining business will be transferred to other providers.

The provider of telephone and online advice, which was set up 15 years ago, announced in July that it was withdrawing from all its contracts to run the NHS’s 111 urgent advice telephone service in England, because the contracts were not financially sustainable.1

NHS Direct was set up under Labour in 1998 as a professionally staffed 0845 number (usually free to callers) to provide health information and advice. In 2010 the Conservative government decided that the 0845 service was too expensive for NHS Direct and would be scrapped, to be replaced by …
Study finds why hospitals are failing to confront medical errors
Authors: B. Roehr
Journal: BMJ (2012)

Researchers have identified why a model that helps to promote patients’ safety and reduce malpractice costs is not being more widely adopted by hospitals.

The University of Michigan Health System pioneered the three principle approach of “disclose, apologize, offer” more than a decade ago. The model proactively identifies unanticipated clinical outcomes, monitors their investigation and changes in practice, communicates with patients and manages remedial care, and offers compensation when warranted.

A new study published …
Baby milk company fined for advertising direct to consumers
Authors: C. Laurent
Journal: BMJ (2003)

SMA Nutrition, part of Wyeth, one of the world's biggest manufacturers of infant formula milk, was last week fined a total of £26 000 ($41 900; €37 200) and ordered to pay costs of more than £34 000 after being convicted of six separate breaches of illegal advertising direct to consumers.

In what is thought to be the first case of its kind, the company was prosecuted by Birmingham Trading Standards at Birmingham Magistrates Court. Judge Rod Ross described the company's breaches as “cynical and deliberate.”

A mother in Birmingham drew the Trading Standards' attention to the advertisement in …
Epidemiology of Blunt Head Trauma in Children in U.S. Emergency Departments
Authors: Kimberly S. Quayle, Elizabeth C. Powell, Prashant Mahajan, John D. Hoyle, Frances M. Nadel, Mohammed K. Badawy, Jeff E. Schunk, Rachel M. Stanley, Michelle Miskin, Shireen M. Atabaki, Peter S. Dayan, James F. Holmes, Nathan Kuppermann
Journal: New England Journal of Medicine (2014)

Among more than 43,000 children treated in 25 emergency departments for blunt head trauma, traumatic brain injury was identified on CT scan in 7% of the patients. Falls were the most frequent injury mechanism for children under the age of 12 years.
TCT-229 Efficacy of an Embolic Protection Stent as a Function of Symptom Onset to Balloon Time in STEMI: The MASTER Trial
Authors: Dariusz Dudek, Alexandre Abizaid, Sigmund Silber, Elad Yaacoby, Joe Dizon, Ricardo A. Costa, Akiko Maehara, Ovidiu Dressler, Sorin Brener, Gregg Stone
Journal: Journal of the American College of Cardiology (2013)
Elevated Ambulatory and Normal Office Blood Pressure
Authors: Thomas G. Pickering, Jennifer E. Liu, Mary J. Roman
Journal: Annals of Internal Medicine (2000)

Letters16 May 2000Elevated Ambulatory and Normal Office Blood PressureThomas G. Pickering, MD, Jennifer E. Liu, MD, and Mary J. Roman, MDThomas G. Pickering, MDSearch for more papers by this author, Jennifer E. Liu, MDSearch for more papers by this author, and Mary J. Roman, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-132-10-200005160-00020 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail IN RESPONSE:Dr. Goldman raises an interesting point about the clinical utility of postural changes in blood pressure. As he is no doubt aware, measuring blood pressure in the standing position is not a routine part of the clinical examination, but perhaps it should be. The current U.S. guidelines from the Joint National Committee on the Prevention, Detection, Evaluation, and Treatment of High Blood Pressure only recommend measuring the effects of postural change on blood pressure “under special circumstances” (mainly in elderly patients and diabetic patients, to look for postural hypotension) (1). The exaggerated increase in diastolic blood pressure (postural ...References1. . The sixth report of the Joint National Committee on the Prevention, Detection, Evaluation, and Treatment of High Blood Pressure. Arch Intern Med. 1997;157:2413-46. CrossrefMedlineGoogle Scholar2. Sparrow D, Tifft CP, Rosner B, Weiss ST. Postural changes in diastolic blood pressure and the risk of myocardial infarction: the Normative Aging Study. Circulation. 1984;70:533-7. CrossrefMedlineGoogle Scholar Author, Article, and Disclosure InformationAuthors: Thomas G. Pickering, MD; Jennifer E. Liu, MD; Mary J. Roman, MDAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoCardiac and Arterial Target Organ Damage in Adults with Elevated Ambulatory and Normal Office Blood Pressure Jennifer E. Liu , Mary J. Roman , Riccardo Pini , Joseph E. Schwartz , Thomas G. Pickering , and Richard B. Devereux Elevated Ambulatory and Normal Office Blood Pressure Arthur G. Goldman Metrics 16 May 2000Volume 132, Issue 10Page: 842KeywordsBlood pressureElderlyHypertensionHypotensionMedical risk factorsMyocardial infarctionPrimary hypertensionTreatment guidelinesVasoconstriction ePublished: 15 August 2000 Issue Published: 16 May 2000 Copyright & PermissionsCopyright © 2000 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
US lawyers investigate trial that secured license for AstraZeneca's antiplatelet drug
Authors: D. Cohen
Journal: BMJ (2013)

The US Department of Justice is investigating the UK drug company AstraZeneca over a clinical trial that was used to gain marketing approval for its antiplatelet drug ticagrelor (marketed in the United States as Brilinta and in the European Union as Brilique).

The US authorities have asked for documents and information about the Platelet Inhibition and Patient Outcomes (PLATO) trial, published in the New England Journal of Medicine in September 2009.1

This 18 000 participant trial, conducted in 43 countries, reported that ticagrelor with aspirin, when compared with clopidogrel with aspirin, significantly reduced the rate of a composite of deaths from vascular causes, myocardial infarction, or stroke (death rate 9.8% versus 11.7% (hazard ratio 0.84 (95% confidence interval 0.77 to 0.92)).

AstraZeneca said it …
Mediastinal and Retropharyngeal Hemorrhage
Authors: Behrooz Eshaghy
Journal: JAMA (1973)

Upper mediastinal and retropharyngeal hemorrhage complicated retrograde left ventricular catheterization in two patients. The clinical signs of shock associated with upper airway obstruction were dramatic and presented diagnostic problems. In one patient, recognition of an enlarging neck circumference and visual examination of the oral pharynx aided in establishing the proper diagnosis, which was later confirmed by x-ray films of the chest and lateral aspects of the neck. Both patients were successfully treated with blood replacement and maintenance of an adequate airway. Anticoagulant therapy may be a cause of serious hemorrhage following arterial perforation.
Quantitative autoradiographic delineation of the distribution of beta-adrenergic receptors in canine and feline left ventricular myocardium.
Authors: S S Murphree, J E Saffitz
Journal: Circulation Research (1987)

The distribution of adrenergic receptors in specific components of the heart such as vessels and myocytes cannot be determined easily with assays of membranes prepared from homogenates of whole tissue. Accordingly, we characterized the binding of the potent nonsubtype selective antagonist [125iodo]cyanopindolol to beta-receptors in unfixed transmural slices of feline and canine left ventricle. Specific binding ratios greater than 90% were achieved at radioligand concentrations near Kd and greater than 80% at saturating ligand concentrations. Binding of radioligand to receptors in transmural slices was rapid, saturable, stereoselective, and displaceable by antagonists and agonists with the rank order of potency expected of beta-adrenergic receptors. Analysis of binding isotherms indicated maximum binding capacities of 27.8 +/- 6.6 and 40.6 +/- 5.1 fmol/mg tissue protein and dissociation constants of 10.1 +/- 1.8 and 21.3 +/- 1.6 pM in feline and canine ventricular slices, respectively. The distribution of beta-receptors in myocytes and selected vascular components of the heart was determined with quantitative film autoradiography and high resolution computer-based analysis and display of the density of binding sites, maximum binding capacity, and binding affinity measurements. The results of autoradiographic analysis revealed a uniform transmural distribution of receptors in regions composed primarily of ventricular myocytes but an inverse relation between the density of beta-receptors and the diameter of coronary vessels. Large epicardial conductance arteries had half the receptor density of subjacent myocytes; small mural arteries had approximately 60% of the beta-receptor density of nearby myocytes, and the coronary resistance arterioles had the highest receptor density of any vascular compartment, which was equivalent to that of myocytes. The methods developed should be of particular value in characterizing the distribution and function of receptor subtypes and mechanisms of regulation of adrenergic responsiveness in intact myocardium.
Timing of Atropine and Neostigmine in the Reversal of Muscle Relaxants
Authors: J. G. Hannington-Kiff
Journal: BMJ (1969)

The antimuscarinic effects of atropine were studied in 46 patients to whom neostigmine had been given after operation to reverse the action of a muscle relaxant. Neostigmine was given to alternate patients three minutes after, or together with, atropine, and the effects of the two procedures were compared by measuring the secretions which collected in the buccal and oropharyngeal cavities and observing the heart rate.It was found that the glands of the oral cavity were stimulated to a greater extent when neostigmine was given with atropine than after atropine. Any dose of atropine sufficient to inhibit peristaltic movements of the bowel is more than enough to block completely secretion by the salivary glands, and the appearance of some secretion in all cases after the administration of neostigmine suggests that the bowel was at liberty to react to the neostigmine in every case, but perhaps particularly so when atropine and neostigmine were given mixed. The integrity of an anastomosis of the bowel could be endangered by vigorous peristalsis in the early postoperative period.Electrocardiograms in about half the patients from each group confirmed earlier work that the muscarinic effects of neostigmine on the heart can be prevented by giving the atropine either before or together with the neostigmine.