tbuckley/cabot-search huggingface dataset:
Abstract 1324: Modulation of IR induced NFKB activity by manipulation of NOS coupling
Authors: Chris Rabender, Ross Mikkelsen
Journal: Cancer Research (2010)

Abstract The transcriptional activity of NFκβ is in part regulated by nitric oxide (NO) and reactive nitrogen species (RNS). Previously we demonstrated that low doses of ionizing radiation (IR) activate constitutive, Ca2+ dependent NO Synthases (NOS) and the generation of RNS, resulting in protein Tyr nitration and/or Cys s-nitrosylation. Previous studies in our lab demonstrated that nitration of IκBα is critical for the IR-induced activation of NFκβ whereas others have shown that s-nitrosylation of the p65 subunit leads to the inhibition of NFκβ. Here we show that exposing MCF-7 cells to 5Gy IR results not only in Tyr nitration of IκBα but also Cys s-nitrosylation of NFκβ both with a maximum at 30min. post IR. One explanation for the discordant results is that NOS activity is uncoupled, resulting in the production of increased levels of superoxide anion as opposed to NO. Tetrahydrobiopterin (BH4) is a necessary cofactor that stabilizes the enzyme and allows the generation of NO through oxidation of L-arginine. However, if BH4 levels become limiting as found in chronic inflammatory or oxidative conditions, electron transfer is instead shuttled to molecular oxygen resulting in the production of superoxide anion, a scenario in which NOS is described as “uncoupled.” When NOS is uncoupled the resulting increase in superoxide can lead to enhanced levels of peroxynitrite formation, resulting in enhanced IκBα Tyr nitration and NFκβ activity. This evidence has led to the hypothesis that by altering cellular levels of BH4, the levels of Tyr nitration and Cys s-nitrosylation can be manipulated, leading to changes in NFκβ activity. To determine the effects of exogenous BH4 on IκBα nitration in MCF-7 cells, cells were treated with varying concentrations of cell permeable sepiapterin (SP), a precursor of BH4, and exposed to 5Gy IR. The results show that between 100nM-500nM IκBα nitration is all but completely eliminated, resulting in decreased NFκβ activity as assessed by luciferase assay. Initial experiments looking at the effects of SP on p65 s-nitrosylation have been variable. At a minimum, levels of s-nitrosylation remain the same as untreated cells; however, enhancement of s-nitrosylation is seen in some experiments. Studies are ongoing to clarify SP's effects on p65 s-nitrosylation as well as studies to determine SP's effects on tumor cell survival post IR. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1324.
Identification of Genetically Modified Maraba Virus as an Oncolytic Rhabdovirus
Authors: Jan Brun, Dan McManus, Charles Lefebvre, Kang Hu, Theresa Falls, Harold Atkins, John C Bell, J. Andrea McCart, Douglas Mahoney, David F Stojdl
Journal: Molecular Therapy (2010)

To expand our current array of safe and potent oncolytic viruses, we screened a variety of wild-type (WT) rhabdoviruses against a panel of tumor cell lines. Our screen identified a number of viruses with varying degrees of killing activity. Maraba virus was the most potent of these strains. We built a recombinant system for the Maraba virus platform, engineered a series of attenuating mutations to expand its therapeutic index, and tested their potency in vitro and in vivo. A double mutant (MG1) strain containing both G protein (Q242R) and M protein (L123W) mutations attenuated Maraba virus in normal diploid cell lines, yet appeared to be hypervirulent in cancer cells. This selective attenuation was mediated through interferon (IFN)-dependent and -independent mechanisms. Finally, the Maraba MG1 strain had a 100-fold greater maximum tolerable dose (MTD) than WT Maraba in vivo and resulted in durable cures when systemically administered in syngeneic and xenograft models. In summary, we report a potent new oncolytic rhabdovirus platform with unique tumor-selective attenuating mutations. To expand our current array of safe and potent oncolytic viruses, we screened a variety of wild-type (WT) rhabdoviruses against a panel of tumor cell lines. Our screen identified a number of viruses with varying degrees of killing activity. Maraba virus was the most potent of these strains. We built a recombinant system for the Maraba virus platform, engineered a series of attenuating mutations to expand its therapeutic index, and tested their potency in vitro and in vivo. A double mutant (MG1) strain containing both G protein (Q242R) and M protein (L123W) mutations attenuated Maraba virus in normal diploid cell lines, yet appeared to be hypervirulent in cancer cells. This selective attenuation was mediated through interferon (IFN)-dependent and -independent mechanisms. Finally, the Maraba MG1 strain had a 100-fold greater maximum tolerable dose (MTD) than WT Maraba in vivo and resulted in durable cures when systemically administered in syngeneic and xenograft models. In summary, we report a potent new oncolytic rhabdovirus platform with unique tumor-selective attenuating mutations.
Abstract 5196: Different miRNAs expression in leiomyosarcoma and undifferentiated pleomorphic sarcoma
Authors: Laura Pazzaglia, Mohamed Guled, Ioana Borze, Maria Serena Benassi, Piero Picci, Sakari Knuutila
Journal: Cancer Research (2014)

Abstract Rare and highly aggressive adult soft tissue sarcomas, leiomyosarcoma (LMS) and undifferentiated pleomorphic sarcoma (UPS), contain complex genomics characterized by a multitude of rearrangements, amplifications and deletions. Since differential diagnosis between the two sarcoma histotypes continues to remain a challenge, we attempted to identify a specific signature useful for improving the differential diagnosis, and we performed miRNA profiling on series of LMS and UPS samples stored at Rizzoli biobank. First, we investigated the different miRNA expression profile in 20 primary sarcoma samples, 10 high-grade LMS and 10 UPS, MFH-like, using miRNA array technique. The top 5 differentially expressed (p&amp;lt;0.01) miRNAs (miR-199b-5p, miR-320a, miR-199a-3p, miR-126, miR-22) were validated by RT-PCR on a series of further 27 UPS and 21 LMS. Confirming our microarray data, an higher expression of miR-199-5p and a lower expression of miR-320a was seen in UPS when compared to LMS. To evaluate the potential role of the above selected miRNAs, the gene targets recognized by three different target prediction databases (miRBase, TargetScan , miRanda) were combined with a list of LMS- and UPS-associated genes reported in literature. From an integrated analysis, IMP3, ROR2, MDM2, CDK4, and UPA genes were identified. By immunohistochemistry analysis, most of them showed strong protein expression in LMS group. Our data suggest a potential role of miR-320a and miR-199-5p in LMS and UPS development and expand our knowledge about these complex sarcoma histotypes. Association analysis between expression and chromosomal locations of miRNAs are ongoing to identify chromosomal regions involved in these tumors. Citation Format: Laura Pazzaglia, Mohamed Guled, Ioana Borze, Maria Serena Benassi, Piero Picci, Sakari Knuutila. Different miRNAs expression in leiomyosarcoma and undifferentiated pleomorphic sarcoma. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5196. doi:10.1158/1538-7445.AM2014-5196
MRC trial of treatment of mild hypertension
Authors: S. Peart, G. Greenberg
Journal: BMJ (1985)

Thank you for your interest in spreading the word about The BMJ.NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address. Username * Your Email * Send To * You are going to email the following MRC trial of treatment of mild hypertension Your Personal Message CAPTCHA This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Transplantation of Human Bone Marrow Mesenchymal Stem Cell Ameliorates the Autoimmune Pathogenesis in MRL/lpr Mice
Authors: Kangxing Zhou, Huayong Zhang, Ouyang Jin, Xuebing Feng, Genhong Yao, Yayi Hou, Lingyun Sun
Journal: Cellular and Molecular Immunology (2008)
CD: Cardiovascular Medicine: Enhanced Multimedia CD-ROM
Authors: B. D Prendergast
Journal: BMJ (2000)

Ed Eric J Topol Lippincott Raven, £182.50 ISBN 0 7817 1681 0 Rating: ![Graphic][1]</img> ![Graphic][2]</img> ![Graphic][3]</img> As we embrace, with varying enthusiasm, the era of desktop publishing, electronic journals with collaborative online links, and the ubiquity of email and the internet, what role remains for the textbook and, specifically, the electronic mega-text? This question is echoed in Professor Topol's preface: “Another textbook in Cardiology—why?” Cardiovascular medicine is a rapidly evolving specialty and has witnessed radical changes in … [1]: /embed/inline-graphic-1.gif [2]: /embed/inline-graphic-2.gif [3]: /embed/inline-graphic-3.gif
THE FATE OF ORALLY ADMINISTERED SOLUBLE SPECIFIC SUBSTANCE OF PNEUMOCOCCUS TYPES I, II AND III
Authors: Victor Ross
Journal: The Journal of Experimental Medicine (1932)

1. Rats, when fed the soluble specific substance of Types I, II and III pneumococcus, excrete a very large proportion in the feces. 2. Following ingestion of these substances, tests sensitive enough to detect 1 part in 2,000,000 of serum, and 1 part in 3,000,000 of urine, were negative. 3. The polysaccharide of Type I, when recovered from the feces, is as active an immunizing agent as it was originally.
The Comparative Effect of Administration of Substances Via the Hepatic Artery or Portal Vein on Hepatic Arterial Resistance, Liver Blood Volume and Hepatic Extraction in Cats
Authors: Wayne W. Lautt, Dallas J. Legare, Timothy R. Daniels
Journal: Hepatology (1984)

Compounds reaching the liver do so via either the hepatic artery or the portal vein. This paper reports on the effectiveness of administration of compounds into these alternate routes for their effects on the hepatic parenchymal cells, the hepatic arterial resistance vessels (blood flow) and hepatic capacitance (blood volume responses). All tests were done on cats under pentobarbital anesthesia. Perfusion of the parenchymal cell mass was assessed by comparing the hepatic elimination of indocyanine green (ICG) administered via the two vascular routes. The ICG uptake was assessed by measuring relative areas under the hepatic venous outflow curve obtained following bolus injections of ICG into the artery and portal vein. In a separate series, using different methods, the hepatic venous levels reached early (2 min) and later (5 min) during a constant infusion were compared during administration via the two routes and found to be equal. Parenchymal cell functions (ICG extraction, bile salt stimulation of bile flow) indicate that blood from the artery and portal vein supplies the hepatic parenchymal cells equally well. This suggests a well-mixed blood supply prior to exposure of either blood stream to parenchymal cells. Substances being processed by the liver are thus equally well handled if reaching the liver via either the arterial or portal blood stream. This has significance in validating the use of some isolated liver perfusion methods that perfuse only via the portal vein. Access of vasoactive compounds in the two blood streams to hepatic arterial resistance vessels was assessed using electromagnetic flow probes. The infusion of adenosine and angiotensin produced dilation and constriction respectively of the hepatic artery with the effect being about 3 times as large at a given dose when infused via the artery. The data show that vasoactive substances in the portal blood can affect the hepatic arterial blood flow by “transvascular” route and that portal blood has quite a high access to hepatic arterial resistance vessels. It does indicate, however, that the arterial resistance sites are not being perfused with mixed portal and arterial blood. The hepatic blood volume changes in responses to intraportal and intraarterial infusions of angiotensin and noradrenaline were measured using an in vivo plethysmograph assembled around the intact liver. Constriction of hepatic blood volume was equal with administration via the two routes indicating that the capacitance vessels are perfused by well-mixed blood and are, therefore, likely to consist mostly of sinusoidal and postsinusoidal vessels with presinusoidal roles being minor or absent.
Spanish doctors on trial for drug fraud
Authors: X. Bosch
Journal: BMJ (1998)

The trial has opened in Barcelona of 13 doctors, three pharmacists, and two pharmaceutical company directors charged with defrauding the Catalonian Health Department of £60000 ($100000). The fraud involved dispensing cheap drugs to patients and claiming back from the state for expensive ones.

The scheme was allegedly masterminded by two brothers, Agustin and Juan Carlos Font Mas, who ran a small pharmaceutical company called …
Points: Renal artery thrombosis caused by antihypertensive treatment
Authors: L. E Ramsay
Journal: BMJ (1983)

December, p 1617) of a patient in whom atenolol treatment apparently precipitated renal artery thrombosis.Recent reports suggest that the angiotensin converting enzyme inhibitor captopril may cause the same complication in patients with pre-existing renal artery stenosis.Oelkers' has described two patients with renovascular hypertension who developed renal artery occlusion during treatment with captopril, and, according to Hollenberg,' about 10% of patients with bilateral renal artery stenosis or stenosis of the artery to a solitary kidney developed rapidly progressive renal failure during the first few weeks of treatment with captopril.Half of these patients recovered renal function with a reduction in dosage of captopril.No details of the remaining patients were given, but one might speculate that some or all of them may also have developed renal artery thrombosis.
British government steps up fight against drugs
Authors: C Court
Journal: BMJ (1994)

The government has unveiled a new strategy for tackling the problems of drug misuse in Britain. It will involve a continued tough approach to those who peddle drugs but will offer more information and education to young people.

The backbone of the campaign, which was launched as part of European drugs prevention week, is a document backed by ministers from the departments of health and education, the Home Office, the Foreign …
DOUBLE TUBAL PREGNANCY WITH RUPTURE OF BOTH TUBES
Authors: Fred E. Clow
Journal: JAMA (1926)

This case is reported because of two unusual features: the simultaneous occurrence of pregnancy in the two fallopian tubes, and rupture of both tubes with a considerable interval separating the two accidents. H. U., an unmarried woman, aged 41, was taken suddenly ill, June 26, with severe cutting pain in the right lower quadrant of the abdomen, with faintness and nausea. The pain occurred while lifting a heavy chair. The physician who was called did little, evidently, beyond giving a hypodermic injection of a narcotic. The patient stayed in bed for three days and then returned to work. She noticed only some tenderness of the abdomen and general muscular weakness. July 10, a similar seizure occurred with pain in the left lower quadrant of the abdomen, faintness and vomiting. There was a slight vaginal discharge of dark blood. The same physician was consulted, but no treatment was prescribed save rest
Report: FDA Exerts Too Little Oversight of Researchers’ Conflicts of Interest
Authors: Bridget M. Kuehn
Journal: JAMA (2009)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Household Pesticides and the Risk of Wilms Tumor
Authors: Maureen A. Cooney, Julie L. Daniels, Julie A. Ross, Norman E. Breslow, Brad H. Pollock, Andrew F. Olshan
Journal: Environmental Health Perspectives (2006)

Previous epidemiologic studies have suggested that exposure to pesticides in utero and during early childhood may increase the risk for development of childhood cancer, including Wilms tumor, a childhood kidney tumor.In this analysis we evaluated the role of residential pesticide exposure in relation to the risk of Wilms tumor in children using data from a North American case-control study.The National Wilms Tumor Study Group (NWTSG) collected information on exposure to residential pesticides from the month before pregnancy through the diagnosis reference date using detailed phone interviews from 523 case mothers and 517 controls frequency matched on child's age and geographic region and identified by list-assisted random digit dialing. Pesticides were grouped according to type of pesticide and where they were used.A slightly increased risk of Wilms tumor was found among children of mothers who reported insecticide use [odds ratio (OR) = 1.4, 95% confidence interval (CI), 1.0-1.8; adjusted for education, income, and the matching variables]. Results from all other categories of pesticides were generally close to the null.This study is the largest case-control study of Wilms tumor to date. We were unable to confirm earlier reports of an increased risk for Wilms tumor among those exposed to residential pesticides during pregnancy through early childhood.
Urine culture in the diagnosis of colovesical fistula.
Authors: A Sandison, P A Jones
Journal: BMJ (1993)

£31 353.66.This is the sum earned for the practice by the trainee.On most days the trainee had a midmorning break, when he drank a cup of coffee provided by the practice.The trainee drank about 210 cups of coffee in one year.A 300 g jar of coffee makes 165 cups at a cost of £3.85; 210 cups of coffee will have cost £4.89.The trainee added 25 ml of milk to each cup, a total of 9-2 pints of milk over the year at a cost of £3.33.The total cost of the trainee to the practice was £8.22.The net financial benefit to the practice ofthe trainee was £31 345.44.
The Acquired Immunodeficiency Syndrome and Nonmenstrual Toxic Shock Syndrome
Authors: JOSEPH SPARANO
Journal: Annals of Internal Medicine (1986)

Letters and Corrections1 August 1986The Acquired Immunodeficiency Syndrome and Nonmenstrual Toxic Shock SyndromeJOSEPH SPARANO, M.D., ELLEN FERRANTI, M.D.JOSEPH SPARANO, M.D.Search for more papers by this author, ELLEN FERRANTI, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-105-2-300_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: We recently saw a patient with the acquired immunodeficiency syndrome (AIDS) in whom we have strong evidence to suspect nonmenstrual toxic shock syndrome, an association that previously has not been described (1).A 26-year-old black homosexual man with a history of furuncles was well until August 1985, when he presented to another hospital withPneumocystis cariniipneumonia. He did well until January 1986, when 3 days before admission he developed pain and swelling on the inner side of his left thigh. One day before admission he developed fever, generalized severe myalgias, pharyngitis, conjunctivitis, abdominal cramps, diarrhea, nausea,...References1. ARMSTRONGGOLDDRYJANSKI DJJ. Treatment of infections in patients with the acquired immunodeficiency syndrome. Ann Intern Med. 1985;103:738-43. LinkGoogle Scholar2. SMITHJACOBSON CJ. Toxic shock syndrome. DM. 1986;32:77-118. MedlineGoogle Scholar3. REINGOLDHARGRETTSHANDS ANK. Toxic shock syndrome surveillance in the United States, 1980 to 1981. Ann Intern Med. 1982;96(6 pt 2):875-80. LinkGoogle Scholar4. REINGOLDHARGRETTDANSHANDSSTRICKLANDBROOME ANBKBC. Nonmenstrual toxic shock syndrome: a review of 130 cases. Ann Intern Med. 1982;96(6 pt 2):871-4. LinkGoogle Scholar5. TODDRESSMANCOSTON JMS. Corticosteroid therapy for patients with toxic shock syndrome. JAMA. 1984;252:3399. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: St. Vincent's Hospital and Medical Center New York, NY 10011 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByTOXIC SHOCK SYNDROMELack of muco-cutaneous signs of toxic shock syndrome when T cells are absent: S. aureus shock in immunodeficient adults with multiple myelomaINFECTIOUS DISEASE UPDATEToxin-mediated streptococcal and staphylococcal diseaseDIAGNOSIS AND TREATMENT OF INFECTIOUS DISEASES IN HIV-INFECTED HOSTSAbdominal Pain and Anorectal Disease in AIDSStaphylococcus and staphylococcal infections. 5. Toxins of Staphylococcus aureus and toxin related diseases. 1 August 1986Volume 105, Issue 2Page: 300-300KeywordsAIDSDiarrheaFeversHomosexualsInflammationMyalgiaNauseaPharyngitisPneumocystis carinii pneumoniaToxic shock syndrome Issue Published: 1 August 1986 PDF DownloadLoading ...
Relationship Between the Occurrence of Cysteine in Proteins and the Complexity of Organisms
Authors: Attila Miseta, Peter Csutora
Journal: Molecular Biology and Evolution (2000)

The occurrence and relative positions of cysteine residues were investigated in proteins of various species. Considering random mathematical occurrence for an amino acid coded by two codons (3. 28%), cysteine is underrepresented in all organisms investigated. Representation of cysteine appears to correlate positively with the complexity of the organism, ranging between 2.26% in mammals and 0. 5% in some members of the Archeabacteria order. This observation, together with the results obtained from comparison of cysteine content of various ribosomal proteins, indicates that evolution takes advantage of increased use of cysteine residues. In all organisms studied except plants, two cysteines are frequently found two amino acid residues apart (C-(X)(2)-C motif). Such a motif is known to be present in a variety of metal-binding proteins and oxidoreductases. Remarkably, more than 21% of all of cysteines were found within the C-(X)(2)-C motifs in ARCHEA.: This observation may indicate that cysteine appeared in ancient metal-binding proteins first and was introduced into other proteins later.