tbuckley/cabot-search huggingface dataset:
Abstract 1084: c-Crk proto-oncogene and TGF-β signaling pathway contribute to transcriptional repression of <i>p120-catenin</i> in non-small cell lung cancer cells
Authors: Fariborz (Fred) Mortazavi, Steven M. Dubinett, Matthew B. Rettig
Journal: Cancer Research (2011)

Abstract As a member of adherens junction, p120-catenin (p120ctn) plays a major role in cell adhesion through stabilization of E-cadherin. p120ctn is transcriptionally down-regulated in non-small cell lung cancer (NSCLC), although the molecular mechanisms underlying p120ctn repression are incompletely defined. Here we further investigated transcriptional regulation of p120ctn in NSCLC. We prepared a promoter reporter plasmid construct that contained p120ctn promoter region from position -1082 to +320 relative to transcription start site. Through serial deletion mutation analysis of the p120ctn promoter, we pinpointed cis-acting elements involved in regulation of p120ctn. We identified transcription factor SP1 as a transcriptional repressor of p120ctn that directly binds to segment (−9 to +36) of the p120ctn promoter. SP1 can receive multiple signals from several intracellular signaling pathways. Through examination of SP1 binding partners, we identified proto-oncogene c-Crk and TGF-β signaling pathway to be involved in transcriptional down-regulation of p120ctn. RNAi mediated silencing of CRK in A549, H157 and H358 cells increased p120ctn protein levels. On the other hand, over-expression of CRK-I in H358 cells down-regulated p120ctn, an effect that was abrogated by simultaneous silencing of SP1. We also observed that CRK-II serine phosphorylation was inversely correlated with p120ctn expression level. This finding suggests a role for serine/threonine kinases in promoting metastasis through phosphorylation of CRK I/II and subsequently down-regulation of p120ctn. In summary, our data provide evidence for the role of c-Crk proto-oncogene and TGF-β signaling in transcriptional repression of p120ctn that further clarifies the mechanism by which these biochemical signals promote metastasis in NSCLC. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1084. doi:10.1158/1538-7445.AM2011-1084
Treatment of chronic actinic dermatitis with azathioprine
Authors: I.M. LEIGH, J.L.M. HAWK
Journal: British Journal of Dermatology (1984)

Fourteen patients with severe unremitting chronic actinic dermatitis were treated with oral azathioprine in a dosage of 100-200 mg daily for a mean 11.5 months. Clinical improvement, apparently permanent, occurred after a few weeks to several months and continued for up to 2 years. Nine patients cleared or improved markedly, one patient cleared and then relapsed again on treatment, two patients showed no response and two patients needed to discontinue therapy because of gastrointestinal side-effects. We feel that in many patients azathioprine can be a very effective therapy for this extremely incapacitating disease.
Indinavir and Interstitial Nephritis
Authors: Mario Sarcletti
Journal: Annals of Internal Medicine (1998)

Letters15 February 1998Indinavir and Interstitial NephritisMario Sarcletti, MD, Anton Petter, MD, and Robert Zangerle, MDMario Sarcletti, MDUniversity of Innsbruck; 6020 Innsbruck, AustriaSearch for more papers by this author, Anton Petter, MDUniversity of Innsbruck; 6020 Innsbruck, AustriaSearch for more papers by this author, and Robert Zangerle, MDUniversity of Innsbruck; 6020 Innsbruck, AustriaSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-128-4-199802150-00021 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:Kopp and colleagues [1] report that indinavir crystalluria is associated with a novel syndrome that consists primarily of back or flank pain with crystalluria but without nephrolithiasis. In four of the patients who had this syndrome, renal parenchymal defects were shown on computed tomography. The authors argue that it is unknown whether patients with this complication subsequently develop chronic deficits in renal function, because serum creatinine levels were only mildly and transiently elevated in two of the patients. However, therapy was discontinued permanently in two patients and continued at a lower dose in another. Only one male ...References1. Kopp JB, Miller KD, Mican JA, Feuerstein IM, Vaughan E, Baker C, et al. Crystalluria and urinary tract abnormalities associated with indinavir. Ann Intern Med. 1997; 127:119-25. Google Scholar2. Tashima KT, Horowitz JD, Rosen S. Indinavir nephropathy [Letter]. N Engl J Med. 1997; 336:138-40. Google Scholar Author, Article, and Disclosure InformationAffiliations: University of Innsbruck; 6020 Innsbruck, Austria PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byAcute interstitial nephritis caused by lopinavir/ritonavir in a surgeon receiving antiretroviral postexposure prophylaxisAntiviral agentsNephrotoxicity of antiretroviral therapy in an HIV-infected patientPersistent Leukocyturia and Loss of Renal Function in a Prospectively Monitored Cohort of HIV-Infected Patients Treated with IndinavirAntiviral agentsIndinavir nephropathy revisited: A pattern of insidious renal failure with identifiable risk factorsIs indinavir crystalluria an indicator for indinavir stone formation?Symptomatic Crystalluria Associated with IndinavirProspective study of urinalysis abnormalities in HIV-positive individuals treated with indinavirRenal toxicity of protease inhibitors 15 February 1998Volume 128, Issue 4Page: 320KeywordsBiopsyComputed axial tomographyCreatinineInflammationIntravenous pyelographyKidneysLower back painNephritis Issue Published: 15 February 1998 Copyright & PermissionsCopyright © 1998 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
CII-DC-AdTRAIL cell gene therapy inhibits infiltration of CII-reactive T cells and CII-induced arthritis
Authors: Zhongyu Liu, Xin Xu, Hui-Chen Hsu, Albert Tousson, Ping-Ar Yang, Qi Wu, Cunren Liu, Shaohua Yu, Huang-Ge Zhang, John D. Mountz
Journal: Journal of Clinical Investigation (2003)

Previously, we described an APC-adenovirus (APC-Ad) FasL cell gene therapy method which could be used to deplete autoreactive T cells in vivo. FasL was toxic, however, and controlled regulation of FasL was not achieved. Here we describe an improved approach to delivering TNF-related apoptosis-inducing ligand (TRAIL) in vivo in which collagen II–induced (CII-induced) arthritis–susceptible (CIA-susceptible) DBA/1j mice were treated with CII-pulsed DCs that had been transfected with a novel Ad system. The Ad was engineered to exhibit inducible TRAIL under the control of the doxycycline-inducible (DOX-inducible) tetracycline response element (TRE). Four groups of mice were treated with CII-DC-AdTRAIL+DOX, CII-DC-AdTRAIL (no DOX), CII-DC-AdGFP+DOX, or DC-AdTRAIL+DOX (no CII), beginning 2 weeks after priming with CII in CFA. The incidence of arthritis and infiltration of T cells in the joint was significantly decreased in CII-DC-AdTRAIL+DOX–treated mice. The in vitro splenic T cell proliferative response and induction of IFN-γ to bovine CII stimulation were also significantly reduced in mice treated with CII-DC-AdTRAIL+DOX. AdTRAIL+DOX was not toxic to DCs or mice but could induce activated T cells to undergo apoptosis in the spleen. Our results suggest that CII-DC-AdTRAIL+DOX cell gene therapy is a safe and effective method for inhibiting the development of CIA.
The Residency II
Authors: C. H. Burnett
Journal: New England Journal of Medicine (1964)

THE department of medicine of a university teaching hospital has a variety of educational responsibilities. These include the provision of basic education in; clinical medicine to undergraduate medical students; internship and residency training for the next generation of community practitioners of internal medicine and of consultants, teachers and investigators in the medical subspecialties; and continuing education for practicing physicians. The ultimate aim of all these educational endeavors is to achieve the maximum positive effect on the availability and quality of medical care for the future. Therefore, it is the obligation of these departments periodically to re-examine the extent of their . . .
Induced-Tolerogenic Dendritic Cells That Promote Tolerance And De Novo Differentiation Of Regulatory T Cells
Authors: R.A. Maldonado, L.C. Perdomo, F. Vascotto, L. Francisco, A.T. Bauquet, V.K. Kuchroo, A.H. Sharpe, U.H. von Andrian
Journal: Journal of Allergy and Clinical Immunology (2012)
1348 VIRAL HETEROGENEITY IN HCV NS5A GENOME IS LOW IN PATIENTS WITH FAVORABLE IL28B GENOTYPES
Authors: M. von Wagner, C. Welsch, E. Herrmann, C. Sarrazin, A. Vermehren, S. Bibert, J.-M. Pawlotsky, A. Neumann, C. Ferrari, S. Schalm, S. Zeuzem
Journal: Journal of Hepatology (2011)
The Electrocardiographic Pattern of Right Ventricular Hypertrophy in Mitral Valve Disease
Authors: RALPH C. SCOTT, SAMUEL KAPLAN, NOBLE O. FOWLER, WILLIAM J. STILES
Journal: Circulation (1955)

The electrocardiograms in 32 patients with mitral valve disease have been compared with the pulmonary resistances, the pulmonary artery pressures, and the size of the mitral valve orifice. In patients with pure stenosis if the electrocardiogram showed the pattern of right ventricular hypertrophy the total pulmonary resistance was usually 1000 dynes seconds cm. –5 or greater. If mitral insufficiency and/or aortic valve lesions were present in addition to mitral stenosis the total pulmonary resistance often exceeded 1000 dynes seconds cm. –5 without right ventricular hypertrophy being evident in the electrocardiogram.
How Does the Ureteric Bud Branch?
Authors: Sanjay K. Nigam, Mita M. Shah
Journal: Journal of the American Society of Nephrology (2008)

Many genes that modulate kidney development have been identified; however, the molecular interactions that direct arborization of the ureteric bud (UB) remain incompletely understood. This article discusses how "systems" approaches may shed light on the structure of the gene network during UB branching morphogenesis and the mechanisms involved in the formation of a branched collecting system from a straight epithelial tube in the context of a stage model. In vitro and genetic studies suggest that the stages seem to be governed by a conserved network of genes that establish a "tip-stalk generator"; these genes sustain iterative UB branching tubulogenesis through minimal alterations in the network architecture as a budding system shifts to one that autocatalytically branches through budding. The differential expression of stage-specific positive and inhibitory factors in the mesenchyme, likely presented in the context of heparan sulfate proteoglycans, and effector molecules in the epithelium seems to regulate advancement between stages; similar principles may apply to other branching epithelia such as the lung, salivary gland, pancreas, mammary gland, and prostate. Active mesenchymal interactions with the UB seem to govern vectorial arborization and tapering of the collecting system and its terminal differentiation. Cessation of branching correlates with induction of mesenchyme as well as local extracellular matrix changes. Perturbations of these mechanisms and/or single-nucleotide polymorphisms in genes regulating UB branching may predispose to a variety of renal diseases (e.g., hypertension and chronic kidney disease) by altering nephron number. Decentralization of the gene-protein interaction network may explain the relative paucity of branching phenotypes in mutant mice and in human disease.
COMPARATIVE EFFICACY OF CEFTRIAXONE AND RIFAMPICIN IN ERADICATING PHARYNGEAL CARRIAGE OF GROUP A NEISSERIA MENINGITIDIS
Authors: Benjamin Schwartz, Abdullah Al-Ruwais, Jalal A'Ashi, ClaireV Broome, Abubakar Al-Tobaiqi, RobertE Fontaine, AllenW Hightower, StanleyI Music
Journal: The Lancet (1988)
Effects of oxygen tension on endothelium dependent responses in canine coronary microvessels
Authors: P. R Myers, J. M Muller, M. A Tanner
Journal: Cardiovascular Research (1991)

Journal Article Effects of oxygen tension on endothelium dependent responses in canine coronary microvessels Get access Paul R Myers, Paul R Myers Correspondence to: Dr Myers Search for other works by this author on: Oxford Academic PubMed Google Scholar Judith M Muller, Judith M Muller Search for other works by this author on: Oxford Academic PubMed Google Scholar Miles A Tanner Miles A Tanner Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 25, Issue 11, November 1991, Pages 885–894, https://doi.org/10.1093/cvr/25.11.885 Published: 01 November 1991 Article history Received: 20 February 1991 Accepted: 02 July 1991 Published: 01 November 1991
Soluble Mesothelin–related Peptides in the Diagnosis of Malignant Pleural Mesothelioma
Authors: Arnaud Scherpereel, Bogdan Grigoriu, Massimo Conti, Thomas Gey, Marc Grégoire, Marie-Christine Copin, Patrick Devos, Bachar Chahine, Henri Porte, Philippe Lassalle
Journal: American Journal of Respiratory and Critical Care Medicine (2006)

Diagnosis of malignant pleural mesothelioma is a challenging issue. Potential markers in mesothelioma diagnosis include soluble mesothelin-related peptides (SMRPs) and osteopontin, but no subsequent validation has been published yet.We prospectively evaluated SMRPs in serum and pleural effusion from patients with mesothelioma (n = 74), pleural metastasis of carcinomas (n = 35), or benign pleural lesions associated with asbestos exposure (n = 28), recruited when first suspected for mesothelioma.Mean serum SMRP level was higher in patients with mesothelioma (2.05 +/- 2.57 nM/L [median +/- interquartile range]) than in patients with metastasis (1.02 +/- 1.79 nM/L) or benign lesions (0.55 +/- 0.59 nM/L). The area under the receiver operating characteristic curve (AUC) for serum SMRP was 0.872 for differentiating mesothelioma and benign lesions, cut-off = 0.93 nM/L (sensitivity = 80%, specificity = 82.6%). The AUC for serum SMRP differentiating metastasis and mesothelioma was 0.693, cut-off = 1.85 nM/L (sensitivity = 58.3%, specificity = 73.3%). SMRP values in pleural fluid were higher than in serum in all groups (mesothelioma: 46.1 +/- 83.2 nM/L; benign lesions: 6.4 +/- 11.1 nM/L; metastasis: 6.36 +/- 21.73 nM/L). The AUC for pleural SMRP-differentiating benign lesions and mesothelioma was 0.831, cut-off = 10.4 nM/L (sensitivity = 76.7%, specificity = 76.2%). The AUC for pleural SMRP-differentiating metastasis and mesothelioma was 0.793.We show that SMRPs may be a promising marker for mesothelioma diagnosis when measured either in serum or pleural fluid. The diagnostic value of SMRPs was similar in both types of samples, but pleural fluid SMRPs may better discriminate mesothelioma from pleural metastasis.
Serum hyaluronan is increased in malignant lymphoma
Authors: Hans Hasselbalch, Doris Hovgaard, Nis Nissen, Peter Junker
Journal: American Journal of Hematology (1995)

Abstract The serum concentration of hyaluronan (HYA) was measured in 41 patients with malignant lymphoma, including 21 patients with non‐Hodgkin's malignant lymphoma and 20 patients with Hodgkin's disease. Thirty‐four patients were studied at diagnosis. The remaining 7 patients had relapsing or resistant disease. The patients were categorized into four stages according to conventional staging procedures. The median serum HYA concentration in patients with malignant lymphoma was significantly higher (median 40.7 ng/ml; 95% confidence limits 26.1–57.6 ng/ml) than in an age‐matched healthy reference group (median 14.5 ng/ml, 95% confidence limits 11–19.4 ng/ml) ( P = 0.00032). The highest serum HYA levels were found in patients with relapsing/resistant disease, all being in stages III and IV (median 181.5; range 11.9–500 ng/ml), as compared to previously untreated patients (median 29.8; range 9.1–108) ( P = 0.0002) and controls (median 14.2; range 6.7–51.2). Decreased uptake and degradation of HYA owing to malignant transformation of lymphatic tissue is the most plausible explanation to these findings.
Gynaecologist botched cosmetic vaginal operations and a termination, GMC hears
Authors: C. Dyer
Journal: BMJ (2011)

A London gynaecologist with a private practice in cosmetic vaginal surgery botched laser operations on four women and left another seriously ill after a termination, a General Medical Council panel was told.

Phanuel Dartey, who came from Ghana and qualified in the former Soviet Union, had operating privileges at two London clinics, the Queen Anne Street Medical Centre, near Harley Street, and the Marie Stopes International Clinic in South Ealing.

He used laser vaginal rejuvenation to heighten …
Low Protein Diets in the Management of Renal Failure
Authors: ROBERT K. MADDOCK
Journal: Annals of Internal Medicine (1968)

Uremic symptoms can be controlled and nitrogen equilibrium established by means of low protein diets, but certain principles of nutrition must be observed. Only "high biologic value proteins" (meat, eggs, and milk) supply balanced quantities of essential amino acids vital for new protein synthesis. "Low biologic value proteins" (starches, cereals, fruits, and legumes) while good calorie sources, impose an unusable nitrogen load. The decrease of dietary protein to a minimum demands a marked increase in calories, vitamin supplementation, and almost exclusive use of "high biologic value protein" at each meal in order to provide enough essential amino acids in proper proportions. A varied, nonformula, 40-g diet that will maintain nitrogen equilibrium is feasible in a hospital. However, outpatients who purchase or prepare their own meals cannot easily reduce their daily protein intake below 60 g unless they are willing to subsist on ice cream.
Stanley Andrew Thomson
Authors: Robin S Thomson
Journal: BMJ (2006)

​FormerFormer general practitioner Stourbridge and Lye (b 1921; q Glasgow 1945), died from pneumonia on 7 February 2006.




Figure 1




After qualifying in Glasgow, Stanley served on troop ships before moving to general practice in Congleton. From 1953 to 1995 he was a vocational general practitioner in Lye and Stourbridge. He was a keen golfer throughout his life and also played university rugby. He leaves a wife, Peggy, and two children.
Outcome of breech delivery at term
Authors: M. Grifftths
Journal: BMJ (1992)

psychological and physical consequences of abdominal delivery other than those directly affect- ing subsequent pregnancies are often overlooked.From a review of the literature Bingham and Lilford concluded that planned abdominal delivery of fetuses presenting by the breech at term might be a safer option for the mother even if only 17% of trials of vaginal breech delivery were unsuccessful.'However, the authors used estimates of maternal mortality and morbidity, and postpartum hysterectomy was the only measure of serious postpartum morbidity.Long term morbidity after discharge from hospital was not addressed.Feldman and Freiman, using a mathematical analysis of American data (Commission on Profes- sional and Hospital Activities 1974 and 1978), calculated that routine elective caesarean section might be safer when the incidence of failed trial of labour was greater than 26%./.They also concluded that the excess maternal mortality resulting from a complete shift of all women in labour to prophy- lactic caesarean section would be 1 in 18000.No measure of morbidity was included in this analysis, and the authors concluded that extrapolating retro- spectively accumulated data to projections in hypothetical new approaches could lead to un- reliable conclusions.Unfortunately, Thorpe-Beeston and colleagues have not told us why the nine "normal" term infants died; possibly, breech presentation was undiagnosed, the mothers presented in advanced labour, or there was cord prolapse.Such accidents would occur even if a policy of wholesale caesarean section was introduced.Certainly, delivery in breech presentations at term should be made safer, but before a shift away from vaginal breech delivery is advocated the impact this would almost certainly have on both morbidity and mortality for the delivering mother must be addressed.
Myocardial Salvage Through Coronary Collateral Growth by Granulocyte Colony-Stimulating Factor in Chronic Coronary Artery Disease
Authors: Pascal Meier, Steffen Gloekler, Stefano F. de Marchi, Andreas Indermuehle, Tobias Rutz, Tobias Traupe, Hélène Steck, Rolf Vogel, Christian Seiler
Journal: Circulation (2009)

The efficacy of granulocyte colony-stimulating factor (G-CSF) for coronary collateral growth promotion and thus impending myocardial salvage has not been studied so far, to our best knowledge.In 52 patients with chronic stable coronary artery disease, age 62+/-11 years, the effect on a marker of myocardial infarct size (ECG ST segment elevation) and on quantitative collateral function during a 1-minute coronary balloon occlusion was tested in a randomized, placebo-controlled, double-blind fashion. The study protocol before coronary intervention consisted of occlusive surface and intracoronary lead ECG recording as well as collateral flow index (CFI, no unit) measurement in a stenotic and a > or =1 normal coronary artery before and after a 2-week period with subcutaneous G-CSF (10 microg/kg; n=26) or placebo (n=26). The CFI was determined by simultaneous measurement of mean aortic, distal coronary occlusive, and central venous pressure. The ECG ST segment elevation >0.1 mV disappeared significantly more often in response to G-CSF (11/53 vessels; 21%) than to placebo (0/55 vessels; P=0.0005), and simultaneously, CFI changed from 0.121+/-0.087 at baseline to 0.166+/-0.086 at follow-up in the G-CSF group, and from 0.152+/-0.082 to 0.131+/-0.071 in the placebo group (P<0.0001 for interaction of treatment and time). The absolute change in CFI from baseline to follow-up amounted to +0.049+/-0.062 in the G-CSF group and to -0.010+/-0.060 in the placebo group (P<0.0001).Subcutaneous G-CSF is efficacious during a short-term protocol in improving signs of myocardial salvage by coronary collateral growth promotion.
Smoking and Cardiac Events After Venous Coronary Bypass Surgery
Authors: Adriaan A. Voors, Ben L. van Brussel, H.W. Thijs Plokker, Sjef M.P.G. Ernst, Nicolette M. Ernst, Egbert M. Koomen, Jan G.P. Tijssen, Freddy E.E. Vermeulen
Journal: Circulation (1996)

Background The long-term clinical effects of smoking and smoking cessation after venous coronary bypass surgery have not been well established. Methods and Results Four hundred fifteen patients who underwent venous coronary bypass surgery between April 1976 and April 1977 were followed up prospectively for 15 years. Multivariate Cox survival analysis revealed that patients who smoked at the time of surgery had no elevated risks for clinical events compared with nonsmokers. However, smoking behavior at 1 and 5 years after surgery appeared to be an important predictor of clinical events during the subsequent follow-up period. Compared with patients who stopped smoking since surgery, smokers at 1 year after surgery had more than twice the risk for myocardial infarction and reoperation. Patients who were still smoking at 5 years after surgery had even more elevated risks for myocardial infarction and reoperation and a significantly increased risk for angina pectoris compared with patients who stopped smoking since surgery and patients who never smoked. Patients who started to smoke again within 5 years after surgery had increased risks for reoperation and angina pectoris. No differences in outcome were found between patients who stopped smoking since surgery and nonsmokers. Conclusions Our results show that smoking cessation after coronary bypass surgery may have important beneficial effects on clinical events during long-term follow-up.