tbuckley/cabot-search huggingface dataset:
Extracellular matrix promotes the growth and differentiation of murine hematopoietic cells in vitro.
Authors: A Campbell, M S Wicha, M Long
Journal: Journal of Clinical Investigation (1985)

We have developed a long-term culture system in which murine marrow cells are cultured on a complex extracellular matrix (ECM) that is derived from marrow and extracted with guanidine hydrochloride and dithiothreitol. Marrow cultures were established with fresh murine marrow cells and recharged at 2 wk (week 0). Phase microscopy showed a dramatically increased adherent cell layer development on ECM compared with controls within a week after recharge. By electron microscopy, this adherent layer was composed of numerous reticular cells apparently attached to the ECM which extended cytoplasmic projections to the surrounding hematopoietic cells. Adherent cellularity on ECM-coated dishes increased to 30 times the control values by week 2. Cumulative suspension cells on ECM dishes were eight times controls. ECM influenced both hematopoietic progenitor cell proliferation and differentiation. Adherent colony-forming unit-granulocyte/macrophage and colony-forming unit-megakaryocyte were greater than 30 and 15 times the control values, respectively, by week 2 (P less than or equal to 0.05). There were more mature granulocytic and megakaryocytic cells in ECM-coated dishes than in controls at all time points. This new culture system directly demonstrates that ECM is an important component of the hematopoietic microenvironment.
Corpus luteum hemorrhage. Cause of acute abdominal pain in patients receiving anticoagulant therapy
Authors: M. Waxman
Journal: JAMA (1978)

Intra-abdominal bleeding from the corpus luteum developed in three women receiving anticoagulant treatment. In one of these patients, no other hemorrhagic manifestations were present. Bleeding from corpus luteum should be considered in differential diagnosis of low abdominal pain in women of reproductive age receiving anticoagulant therapy. The ovary has not been widely recognized as a site of hemorrhage complicating anticoagulant therapy, although abdominal bleeding in this location may be more common than previously thought. Thorough clinical studies are needed to establish the precipitating factors and true incidence of this complication.
Ethological and experimental approaches to behavior analysis: implications for ecotoxicology.
Authors: J Cohn, R C MacPhail
Journal: Environmental Health Perspectives (1996)

Laboratory research in toxicology has progressed far beyond reliance on measures of mortality to make use of sophisticated behavioral preparations that can evaluate the consequences of sublethal toxicant exposure. In contrast, field studies have not evolved as rapidly. Approaches developed by experimental psychologists and ethologists provide powerful and complementary methodologies to the study of environmental pollutants and behavior. Observational data collection techniques can easily be used to broaden the number of questions addressed regarding sublethal exposure to toxic agents in both field and laboratory environments. This paper provides a background in such techniques, including construction of ethograms and observational methodologies, and the use of laboratory analogues to naturally occurring activities such as social behavior, predation, and foraging. Combining ethological and experimental approaches in behavior analysis can result in a more comprehensive evaluation of the effects of environmental contaminants on behavior.
Ultrasound-sensitive siRNA-loaded nanobubbles formed by hetero-assembly of polymeric micelles and liposomes and their therapeutic effect in gliomas
Authors: Tinghui Yin, Ping Wang, Jingguo Li, Rongqin Zheng, Bowen Zheng, Du Cheng, Ruitang Li, Jieyi Lai, Xintao Shuai
Journal: Biomaterials (2013)
<i>In vitro</i> and Clinical Studies of Gene Therapy with Recombinant Human Adenovirus-<i>p53</i> Injection for Oral Leukoplakia
Authors: Yi Li, Long-Jiang Li, Song-Tao Zhang, Li-Juan Wang, Zhuang Zhang, Ning Gao, Yuan-Yuan Zhang, Qian-Ming Chen
Journal: Clinical Cancer Research (2009)

Oral leukoplakia is a well-recognized precancerous lesion of squamous cell carcinoma. When accompanied with abnormal p53 expression, it suffered a higher risk of canceration. The present study was carried out to test whether the recombinant human adenovirus-p53 could introduce wild-type p53 gene to oral leukoplakia cells and induce cell cycle arrest and apoptosis.We select p53(-) oral dysplastic keratinocyte POE-9n, to observe the growth inhibition, cell cycle change, apoptosis-induced effects, and elaborate the corresponding molecular mechanism of recombinant adenovirus-p53 on POE-9n cells. Meanwhile, we evaluate the feasibility, safety, and biological activity of multipoints intraepithelial injections of recombinant adenovirus-p53 in 22 patients with dysplastic oral leukoplakia.Exogenous p53 could be successfully transduced into POE-9n cells by recombinant adenovirus-p53. The optimal infecting titer in this study was multiplicity of infection (MOI) = 100. Recombinant adenovirus-p53 could strongly inhibit cell proliferation, induce apoptosis, and arrest cell cycle in stage G(1) in POE-9n cells by inducing p21(CIP/WAF) and downregulating bcl-2 expression. In the posttreatment patients, p53 protein and p21(CIP/WAF) protein expression were significantly enhanced, yet bcl-2 protein presented low expression. Sixteen patients showed clinical response to the treatment, and 14 patients showed obvious histopathologic improvement.Intraepithelial injections of recombinant human adenovirus-p53 were safe, feasible, and biologically active for patients with dysplastic oral leukoplakia.
Calcified Soft-Tissue Mass in the Medial Aspect of the Thigh
Authors: Thomas E. Shea
Journal: JAMA (1967)

Dr. Kook Sang Oh: This is the case of a 26-year-old sales manager who had enjoyed excellent health. Three years prior to admission he received a severe blow to the medial aspect of his left thigh from an opponent's foot during a touch football game. One year prior to admission he noted a swelling in the same area which was persistent and occasionally tender. A physician was consulted and conservative treatment to relieve the swelling was started but without success. Roentgenograms of the left thigh (Figure) were then obtained. A mass was locally resected from the thigh, and the patient was referred to this hospital for further management. Physical examination was not remarkable except for small, nontender, palpable nodes in the groin and a fresh transverse scar with a drain site on the medial aspect of the left thigh. Laboratory data were within normal limits. <h3>Discussion</h3> Dr. Laurence L. Robbins
Remission of diarrhoea due to cryptosporidiosis in an immunodeficient child treated with hyperimmune bovine colostrum.
Authors: S Tzipori, D Roberton, C Chapman
Journal: BMJ (1986)

A boy aged 6 months who presented with poor weight gain, diarrhoea, and infection with Pneumocystis carinii was found to have congenital hypogammaglobulinaemia, which did not improve despite monthly treatment with intravenous gammaglobulin. At the age of 3 years and 2 months he developed severe vomiting and diarrhoea due to cryptosporidiosis, which failed to respond to conventional treatment. Infusion of hyperimmune bovine colostrum produced against parasite antigen, given by nasogastric tube, was started after symptoms had persisted for three weeks. His vomiting and diarrhoea resolved within five days of treatment, and oocysts were no longer seen in the stools after eight days. Later, however, he developed a rare complication, and oocysts were found in the common bile duct. Hyperimmune bovine colostrum may be useful in the treatment of many patients with immunodeficiency disorders.
Primary Disseminated Multifocal Ewing Sarcoma: Results of the Euro-EWING 99 Trial
Authors: Ruth Ladenstein, Ulrike Pötschger, Marie Cécile Le Deley, Jeremy Whelan, Michael Paulussen, Odile Oberlin, Henk van den Berg, Uta Dirksen, Lars Hjorth, Jean Michon, Ian Lewis, Alan Craft, Heribert Jürgens
Journal: Journal of Clinical Oncology (2010)

To improve the poor prognosis of patients with primary disseminated multifocal Ewing sarcomas (PDMES) with a dose-intense treatment concept.From 1999 to 2005, 281 patients with PDMES were enrolled onto the Euro-EWING 99 R3 study. Median age was 16.2 years (range, 0.4 to 49 years). Recommended treatment consisted of six cycles of vincristine, ifosfamide, doxorubicin, and etoposide (VIDE), one cycle of vincristine, dactinomycin, and ifosfamide (VAI), local treatment (surgery and/or radiotherapy), and high-dose busulfan-melphalan followed by autologous stem-cell transplantation (HDT/SCT).After a median follow-up of 3.8 years, event-free survival (EFS) and overall survival (OS) at 3 years for all 281 patients were 27% +/- 3% and 34% +/- 4% respectively. Six VIDE cycles were completed by 250 patients (89%); 169 patients (60%) received HDT/SCT. The estimated 3-year EFS from the start of HDT/SCT was 45% for 46 children younger than 14 years. Cox regression analyses demonstrated increased risk at diagnosis for patients older than 14 years (hazard ratio [HR] = 1.6), a primary tumor volume more than 200 mL (HR = 1.8), more than one bone metastatic site (HR = 2.0), bone marrow metastases (HR = 1.6), and additional lung metastases (HR = 1.5). An up-front risk score based on these HR factors identified three groups with EFS rates of 50% for score <or= 3 (82 patients), 25% for score more than 3 to less than 5 (102 patients), and 10% for score >or= 5 (70 patients; P < .0001).PDMES patients may survive with intensive multimodal therapy. Age, tumor volume, and extent of metastatic spread are relevant risk factors. A score based on these factors may facilitate risk-adapted treatment approaches.
569 RISK OF SEEDING AND METASTASIS IN THE THORACIC OR ABDOMINAL WALL DUE TO RFA AND BIOPSY OF HCC ON CIRRHOSIS. SINGLE CENTRE EXPERIENCE. CAUTIONARY NOTE
Authors: L.G. Lupo, R.M. Notarnicola, P. Panzera, M. Colasuonno, C. Bizzoca, V. Memeo
Journal: Journal of Hepatology (2010)
IX. Thyrotoxic Storm
Authors: Sidney H. Ingbar
Journal: New England Journal of Medicine (1966)

IN contrast to other endocrine disorders, such as diabetes mellitus and Addison's disease, which not uncommonly constitute acute medical emergencies, diseases of the thyroid gland tend to progress slowly and rarely pose an acute threat to the life of the patient, exceptions being the clinical syndromes of thyrotoxic crisis or storm and myxedema coma. These disorders, despite their relative rarity, hold great interest because their pathophysiology is poorly understood, they are dramatic in their manifestations, and they carry a poor prognosis. This discussion concerns the recognition and treatment of the more common of the two disorders, thyrotoxic storm. This clinical . . .
Lack of Effect of Improved Glycemic Control on C-Peptide Secretion in Patients Without Residual B-Cell Function
Authors: Deborah E Seigler, Michael L Reeves, Jay S Skyler
Journal: Diabetes Care (1982)

Ten patients with insulin-dependent diabetes mellitus underwent a program of intensification of therapy that resulted in marked improvement in glycemic control. C-peptide secretion in response to a test meal and to an intravenous glucagon challenge was used to assess pancreatic B-cell function in terms of residual endogenous insulin secretion. Patients, who had diabetes for a mean (±SEM) of 8.4 ± 1.9 yr, had minimal or no C-peptide response at baseline evaluation. C-peptide responses remained unchanged when reassessed after 2, 4, and 6 mo of improved glycemic control.
Quantitative Evaluation of Circulatory Left-to-right Shunts by Ascorbate Dilution Using a Single Venous Catheter for Injection and Measurement
Authors: B. Oeseburg, E. Meijer, J. Stutterheim, W. G. Zijlstra
Journal: Cardiovascular Research (1969)

Journal Article Quantitative Evaluation of Circulatory Left-to-right Shunts by Ascorbate Dilution Using a Single Venous Catheter for Injection and Measurement Get access B. Oeseburg, B. Oeseburg From the Laboratory of Chemical Physiology, and the Division of Paediatric Cardiology, University of Groningen, the Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar E. Meijer, E. Meijer From the Laboratory of Chemical Physiology, and the Division of Paediatric Cardiology, University of Groningen, the Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar J. Stutterheim, J. Stutterheim From the Laboratory of Chemical Physiology, and the Division of Paediatric Cardiology, University of Groningen, the Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar W. G. Zijlstra W. G. Zijlstra From the Laboratory of Chemical Physiology, and the Division of Paediatric Cardiology, University of Groningen, the Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 3, Issue 2, April 1969, Pages 235–240, https://doi.org/10.1093/cvr/3.2.235 Published: 01 April 1969 Article history Received: 19 August 1968 Published: 01 April 1969
Reply to Dr. Matshushita et al.
Authors: P. Mensink, J. Haringsma
Journal: Endoscopy (2007)

We thank Dr. Matshushita and his colleagues for their interest in our article on complications and double balloon enteroscopy (DBE). In this article we conclude that diagnostic DBE is a relatively safe procedure with an overall complication rate of 0.8 % [1]. Acute pancreatitis was found to be the most common complication after diagnostic DBE (0.3 %). All cases of acute pancreatitis after diagnostic DBE procedures presented in our article occurred after peroral procedures.
Philadelphia chromosome-negative very high-risk acute lymphoblastic leukemia in children and adolescents: results from Children’s Oncology Group Study AALL0031
Authors: K R Schultz, M Devidas, W P Bowman, A Aledo, W B Slayton, H Sather, H W Zheng, S M Davies, P S Gaynon, M Trigg, R Rutledge, D Jorstad, A J Carroll, N Heerema, N Winick, M J Borowitz, S P Hunger, W L Carroll, B Camitta
Journal: Leukemia (2014)
The Legacy of Neglect: An Appraisal of the Implications of Emotional Disturbances in the Business Environment
Authors: Marjorie C. Meehan
Journal: JAMA (1965)

<h3>The Mentally Ill Employee: His Treatment and Rehabilitation.</h3> A Guide for Management. By the Committee on Occupational Psychiatry of the American Psychiatric Association. 110 pp. $2.95. Hoeber Medical Division, Harper &amp; Row, 49 E 33rd St, New York 10016, 1965 Although, as early as 1916, Beulingame studied the effects of emotional maladjustment of employees, there was little real interest in the application of psychiatry to industry until World War II. Since then, a growing number of companies have shown concern about the mental health of their workers and have made various attempts to cope with psychiatric problems. This interest is reflected in two recent publications, both the work of committees, both addressed to executives, personnel workers, and industrial physicians. <i>The Legacy of Neglect</i>was prepared by a group of ten students at the Harvard Graduate School of Business Administration, young men interested in management problems, with, presumably, no significant knowledge
More Good (i.e., Practical) Reviews?
Authors: Franz J. Ingelfinger
Journal: New England Journal of Medicine (1969)

Dear "Old Timer,"Your two suggestions sent to the Journal on a page torn from the Massachusetts Physician raise issues that chronically aggravate the editor's unease. Your first suggestion is that the Journal carry more "good review articles" dealing with "diseases seen daily." In support of this request, you cite respected and knowledgeable backers,1 , 2 but I am not exactly clear what you have in mind. Surely you and Dr. Cummings do not refer, if we take medicine as an example, to any material that can be found in such fine texts as Beeson-McDermott, Harrison, or Harvey's updated Principles and Practice . . .
A novel role for HMGB1 in TLR9-mediated inflammatory responses to CpG-DNA
Authors: Stanimir Ivanov, Ana-Maria Dragoi, Xin Wang, Corrado Dallacosta, Jennifer Louten, Giovanna Musco, Giovanni Sitia, George S. Yap, Yinsheng Wan, Christine A. Biron, Marco E. Bianchi, Haichao Wang, Wen-Ming Chu
Journal: Blood (2007)
RELATIVE DEFICIENCY OF ENDOGENOUS ERYTHROPOIETIN AND INCREASED MUSCLE SYMPATHETIC NERVE ACTIVITY IN PATIENTS WITH CARDIO-RENAL ANEMIA SYNDROME
Authors: Shuji Joho, Yoshitaka Oda, Daisuke Harada, Tadakazu Hirai, Hiroshi Inoue
Journal: Journal of the American College of Cardiology (2010)
Varied Appearance of Cardiac Pseudoaneurysms on Blood Pool Images
Authors: FERRIS GINSBERG, RONALD J. ROSENBERG, JOHN J. SZIKLAS, RICHARD P. SPENCER
Journal: Clinical Nuclear Medicine (1987)

Three cases of cardiac pseudoaneurysms were presented, with findings at the time of gated blood pool imaging. In only one of three was there the finding of a small neck connecting the ventricular cavity with the pseudoaneurysm. In the other two patients, there was a considerably larger communication. The size of the pseudoaneurysm itself was also of little diagnostic assistance. In one case it was small, while in a second patient it was larger in size. The third case revealed a pseudoaneurysm of enormous size (larger than the ventricular cavity). Gated blood pool imaging apparently can be called upon to demonstrate the presence of an abnormality. It may not be able to definitely establish the diagnosis of pseudoaneurysm, but should suggest the use of cardiac angiography to distinguish these life threatening lesions from true aneurysms.
Pulmonary Artery Stenosis Associated with Ductus Arteriosus Following Maternal Rubella
Authors: GEORGE C. EMMANOUILIDES, LEONARD M. LINDE, I. HUNTER CRITTENDEN
Journal: Circulation (1964)

Nine patients with pulmonary artery stenosis associated with patent ductus arteriosus following maternal rubella are described. Five of the patients had bilateral pulmonary artery stenosis and four cases involved only the right pulmonary artery. Mild pulmonary valvular stenosis was present in five and a ventricular septal defect in one. The persistence of a systolic murmur transmitted to the lateral chest wall, after ligation or division of a patent ductus arteriosus, should arouse suspicion of the presence of pulmonary artery stenosis. This report supports the recently described implication of maternal rubella as a cause of pulmonary artery stenosis. Careful auscultation in patients with history of maternal rubella may discover the presence of such an arterial anomaly, but cardiac catheterization and angiocardiography are necessary for substantiation of diagnosis. The natural history of these lesions is not known.
Erectile incontinence post radical prostatectomy
Authors: F C L Banks
Journal: Journal of the Royal Society of Medicine (2006)

A patient who underwent a nerve-sparing radical retropubic prostatectomy, who was post-operatively continent, developed urinary incontinence associated with erectile recovery. The bladder was stable and minimal distortion of the bladder neck was observable on videourodynamics. We suggest that the mechanism of incontinence is urethral traction, induced by an erect penis causing an anatomically minor, but functionally significant opening of the bladder neck/sphincter mechanism. This case highlights the importance of a stable sphincter mechanism in maintaining continence. It also emphasizes the fine line between success and failure in post radical retropubic prostatectomy continence; and is a reminder of the importance of surgical technique in achieving the goal of a cancer free, potent, continent patient.
Review: Combined oral contraceptives are associated with venous thrombosis
Authors: Diane M. Harper, Lauren E.S. Wilfling, Christopher F. Blanner
Journal: Annals of Internal Medicine (2013)

Source Citation Stegeman BH, de Bastos M, Rosendaal FR, et al. Different combined oral contraceptives and the risk of venous thrombosis: systematic review and network meta-analysis. BMJ. 2013;347:f5298. 24030561
Should folic acid fortification be mandatory? No
Authors: Richard A Hubner, Richard D Houlston, Kenneth R Muir
Journal: BMJ (2007)

The UK9s Food Standards Agency recently recommended mandatory folic acid fortification of some foods. <b>Nicholas Wald</b> and <b>Godfrey Oakley</b> argue that it9s a safe effective way of preventing spina bifida and anencephaly—but <b>Richard Hubner and colleagues </b>say that more research into the harms is needed
Myometrial angiotensin II receptor subtypes change during ovine pregnancy.
Authors: B E Cox, M A Ipson, P W Shaul, K E Kamm, C R Rosenfeld
Journal: Journal of Clinical Investigation (1993)

Although regulation of angiotensin II receptor (AT) binding in vascular and uterine smooth muscle is similar in nonpregnant animals, studies suggest it may differ during pregnancy. We, therefore, examined binding characteristics of myometrial AT receptors in nulliparous (n = 7), pregnant (n = 24, 110-139 d of gestation), and postpartum (n = 21, 5 to > or = 130 d) sheep and compared this to vascular receptor binding. We also determined if changes in myometrial binding reflect alterations in receptor subtype. By using plasma membrane preparations from myometrium and medial layer of abdominal aorta, we determined receptor density and affinity employing radioligand binding; myometrial AT receptor subtypes were assessed by inhibiting [125I]-ANG II binding with subtype-specific antagonists. Compared to nulliparous ewes, myometrial AT receptor density fell approximately 90% during pregnancy (1,486 +/- 167 vs. 130 +/- 16 fmol/mg protein) and returned to nulliparous values > or = 4 wk postpartum; vascular binding was unchanged. Nulliparous myometrium expressed predominantly AT2 receptors (AT1/AT2 congruent to 15%/85%), whereas AT1 receptors predominated during pregnancy (AT1/AT2 congruent to 80%/20%). By 5 d postpartum AT1/AT2 congruent to 40%/60%, and > 4 wk postpartum AT2 receptors again predominated (AT1/AT2 congruent to 15%/85%). In studies of ANG II-induced force generation, myometrium from pregnant ewes (n = 10) demonstrated dose-dependent increases in force (P < 0.001), which were inhibited with an AT1 receptor antagonist. Postpartum myometrial responses were less at doses > or = 10(-9) M (P < 0.05) and unaffected by AT2 receptor antagonists. Vascular and myometrial AT receptor binding are differentially regulated during ovine pregnancy, the latter primarily reflecting decreases in AT2 receptor expression. This is the first description of reversible changes in AT receptor subtype in adult mammals.
BMJ was out of touch with grass roots doctors on wellbeing
Authors: A. Fogarty
Journal: BMJ (2003)

EDITOR—I resent the way Cross and Brownscombe portrayed me as a “pariah” in their article on the British and Australian view of wellbeing.1 When I was interviewed I had just worked for the previous three nights until past midnight, having also worked Christmas Day and New Year's Day, and without holidays for six …