tbuckley/cabot-search huggingface dataset:
Abstract 3493: Expression of VEGFR and FRGFR on endothelial cells isolated from hepatocellular carcinoma and adjacent liver tissue
Authors: Sun Hui-Chuan, Ling-Qun Kong, Xiao-Dong Zhu, Zong-Tao Chai, Wen-Quan Wang, Wei-Zhong Wu
Journal: Cancer Research (2011)

Abstract Background Heterogenity in gene expression profiles in tumor endothelial cells (TEC) has been reported reported. Our previous studies showed TEC is also different from endothelial cells isolated from adjacent normal liver tissue (NEC) in term of response to chemotherapeutics and anti-angiogenesis drugs. Methods TEC and NEC were isolated by CD31 and CD105 antibody conjuaged magnetic beads from tumor and adjacent nontumor liver tissues from 5 patients who were diagnosed as hepatocellular carcinoma with a positive alpha fetoprotein (&amp;gt; 20ng/ml). The purity of ECs was proved by LDL internalization assay, tube formation, immunostaining and RT-PCR to exclude tumor cell contamination. VEGFR1, VEGFR2, FGFR-2, CD31 and CD105 expression on the isolated ECs were assayed by flow cytometry within 3-5 passages. Response of different types of ECs to sorafenib treatment was observed, including cell proliferation, VEGFRs and FGFR expression. Results The isolated ECs were proved by tumor formation and LDL internalization. More than 97% cells are LDL positive. AFP was not detectable on the isolated ECs in both flow cytometry assay and immunostaining assay. VEGFRs and FGFR expression were different on CD105+TEC and CD31+TEC among different patients, ranging from 18% to 67% for VEGFR1, 1% to 8% for VEGFR2, and 18% to 25% for FGFR-2 on CD105+TEC, and 18% to 90% for VEGFR1, 10% to 40% for VEGFR2, and 18% to 30% for FGFR-2 on CD31+TEC. Expression of VEGFRs and FGFR is higher on CD105+ECs than those on CD31+ECs. While between TEC and NEC, the expression of VEGFRs and FGFR is also heterogeneous. Sorafenib at 5 mM inhibted proliferation of TEC and NEC in a dose-dependent manner, while NEC is more sensitive to sorafenib treatment. Conclusions VEGFRs and FGFR expression were different among different patients; CD105+EC and CD31+EC are probably two types of ECs with different biological features. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3493. doi:10.1158/1538-7445.AM2011-3493
Microanalysis of the First Interview with Psychosomatic Patients
Authors: J. Bastiaans
Journal: Psychotherapy and Psychosomatics (1979)

Microanalysis of the first interview is a technique developed by the author for the subtle translation of the verbal aspects of the doctor-patient contact. Every bit of information, derived from the patient, is translated in the traditional historical dimension, e.g., when did the complaint start, and in the here and now dimension, e.g., in how far does the information imply a cry for help and understanding. Psychosomatic patients, who are usually well adapted, suppress or repress their problems and emotions so much that they are hardly able to express these quite openly. Psychosomatic symptoms reflect the effort of the patient not to behave in a hysterical way. This means that the overt cry for help and attention, which is so characteristic for hysterical patients, is warded off. Especially the microanalytic translation of this hidden cry may be used by the interviewer to detect what the basic problems of the patient are and how the optimal climate for an efficient therapeutic contact can be established. In the Leyden Department of Psychiatry the technique is used to facilitate the training in interview technique. In the paper models will be given for the understanding of the interrelationship of psychiatric and psychosomatic symptoms.
Which type of hospital discharge report reaches general practitioners most quickly?
Authors: A. R. Kendrick, D. J. Hindmarsh
Journal: BMJ (1989)

The introduction of home monitoring of blood glucose concentrations in the 1970s was a major advance.'A ntmber of patients, however, become obsessed with the results and develop a disruptive neurosis.We describe three such patients. Case reportsCase 1-A 53 year old woman was referred with
Outcomes of kidney transplantation are better in Spain than the US, a study finds
Authors: A. G. Rada
Journal: BMJ (2011)

Patients who receive a kidney transplant in Spain are less likely to lose the graft and to die than those in the US, a study has found. 

The researchers, transplant experts from both countries, say that the risk of losing a graft six to 11 years after transplantation was 75% to 89% higher in US patients compared with Spanish patients (P<0.001) while the risk of the recipient dying was 114% to 145% higher in the US cohort (P<0.001). The findings were presented at two national scientific congresses of transplant experts and nephrologists in Spain.

The study, which has been led by Akinlolu Ojo, from the University of Michigan Health System, concludes that …
A Novel Mechanism of Synergistic Cytotoxicity with 5-Fluorocytosine and Ganciclovir in Double Suicide Gene Therapy
Authors: Paul D. Boucher, Michael M. Im, Svend O. Freytag, Donna S. Shewach
Journal: Cancer Research (2006)

Abstract The combination of cytosine deaminase (CD) and herpes simplex virus thymidine kinase (HSV-TK) suicide gene protocols has resulted in enhanced antitumor activity in cultured tumor cells and animal models. In this study, we show that concurrent addition of prodrugs 5-fluorocytosine (5-FC) and ganciclovir (GCV) was less efficacious than sequential treatment in human DU145 prostate carcinoma cells infected with an adenovirus containing a CD/HSV-TK fusion gene. If cells were incubated for 24 hours with 5-FC followed by a 24-hour GCV treatment, GCV triphosphate levels were 2-fold higher, incorporation of GCV monophosphate into DNA was 2.5-fold higher, and growth inhibition was increased 4-fold compared with simultaneous treatment. As expected, cellular dTTP levels were reduced during the 5-FC preincubation. However, dGTP pools also declined parallel to the dTTP decrease. Similar results were obtained when 5-fluorouracil or 5-fluoro-2′-deoxyuridine was used instead of CD/5-FC. These data allowed us to propose a novel hypothesis for the synergistic interaction between CD/5-FC and HSV-TK/GCV treatments. We suggest that the CD/5-FC–mediated reduction of dTTP results in a concurrent decrease of dGTP due to allosteric regulation of ribonucleotide reductase. Because dGTP is the endogenous competitor of GCV triphosphate, depleted dGTP at the time of GCV addition results in increased GCV in DNA and cell kill. In fact, addition of deoxyguanosine during the 5-FC incubation reverses the dGTP depletion, reduces the amount of GCV monophosphate incorporated into DNA, and prevents the CD/5-FC–mediated enhancement of HSV-TK/GCV cytotoxicity. Understanding this mechanistic interaction may help recognize better strategies for creating more efficacious clinical protocols. (Cancer Res 2006; 66(6): 3230-7)
Abstract A14: Understanding the role of ATRX in the development and progression of glioma
Authors: Hannah E. Goldberg, Kristin D. Kernohan, Nathalie G. Berube
Journal: Cancer Research (2013)

Abstract Gliomas are tumors that arise from glial cells within the brain and spinal cord and account for approximately 30% of all central nervous system neoplasms. Emerging evidence indicates that unique molecular and genetic footprints define glioma subtypes. The Atrx gene is commonly mutated in a subset of gliomas across all tumor grades and within both the pediatric and adult populations. ATRX is a chromatin remodeling protein that is necessary for proper neural development. Loss of ATRX conditionally in the forebrain of the mouse starting at embryonic day 8.5 leads to an increase in replication stress and p53-mediated cortical cell death. Microarray analysis of control and Atrx-null neonatal forebrains indicates a significant increase in the expression of many cancer-related genes. Many of these overexpressed genes are involved in cellular migration, invasion, and epithelial-mesenchymal transition — important cellular phenotypes known to contribute to glioma development. Our data highlight oncogenic signaling pathways that become activated in the absence of the ATRX protein in the brain that may promote tumorigenesis when combined with additional genetic mutations, such as p53 mutations. Citation Format: Hannah E. Goldberg, Kristin D. Kernohan, Nathalie G. Berube. Understanding the role of ATRX in the development and progression of glioma. [abstract]. In: Proceedings of the AACR Special Conference on Chromatin and Epigenetics in Cancer; Jun 19-22, 2013; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2013;73(13 Suppl):Abstract nr A14.
P247Molecular alterations in human urine reveal atherosclerosis development, cardiovascular event at onset and follow-up
Authors: G Alvarez-Llamas, M Martin-Lorenzo, I Zubiri, AS Maroto, L Gonzalez-Calero, F De La Cuesta, LF Lopez-Almodovar, LR Padial, MG Barderas, F Vivanco
Journal: Cardiovascular Research (2014)

Purpose: Search of progression and recovery markers in atherosclerosis disease. Methods: An animal model of early atherosclerosis was done in rabbits fed with different diets, regular diet and cholesterol-rich (control and pathological group). Urine samples from animals were analyzed in the search for alterations of the proteome and metabolome (by 2DE-DIGE and NMR, respectively). Confirmation analyses were performed by SRM-(LC-MS/MS). In a pilot translational study, human urine samples from healthy donors and NSTEACS patients at the onset and discharge were analyzed. Results: Four proteins were found significant altered between the two animal groups. Cathepsin D is increased in urine from pathological animals and Hemopexin, Kallikrein 1 (KLK1) and Zymogen granule protein 16B (ZG16B) are decreased. After translational studies KLK1 and ZG16B are proposed to be markers of both, progression and recovery. NMR pops up twenty metabolites significantly varied at early stages of atherosclerosis between animal groups. Eight metabolites increased in urine from pathological animals and twelve metabolites showed the opposite trend (decreased). Two panels of six and three metabolites are found as responders to atherosclerosis progression or recovery from an acute event, respectively. Metabolism changes of particular sugars, hydroxy acids, amino acids, cyclic alcohols, polyamines and imidazolidines are here shown to be associated to atherosclerosis. Conclusions: These data confirm that omics approaches are extremely valuable for the diagnosis and monitoring of atherosclerotic pathologies. Four proteins and twenty metabolites are significantly altered in an early animal model of atherosclerosis. In human samples, two novel urinary panels of proteins and metabolites are here first shown able to monitor atherosclerosis progression and recovery from an acute event.
Abstract 485: Epidermal growth factor-like repeats of Tenascin C promote melanoma cell invasion, possibly by enabling mesenchymal to ameboid migration transition
Authors: Jelena Grahovac, Alan Wells
Journal: Cancer Research (2012)

Abstract In malignant melanoma, the most dangerous skin cancer, Tenascin-C (TNC) is overexpressed, especially at the invasive fronts of invasive melanomas. We found that the migration and invasiveness of a set of melanoma cell lines correlates with levels of TNC expression suggesting, surprisingly, that the melanoma cells themselves provide much of this matrix component. Adding TNC to matrices enhanced individual cell migration in cells that did not express much TNC, whereas it did not enhance the rapid motility of the cells expressing high levels. As TNC is a multidomain protein, the signaling pathways that promote this motogenic invasiveness are not obvious. Herein, we examined the potential role of the EGF-like repeats of TNC that have been shown to preferentially activate motogenic-signaling cascades. To analyze the effects of EGFL signaling on melanoma migration and invasion, we overexpressed TNC fragment containing the assembly and EGFL domains of TNC (TNCEGFL). Interestingly, this led to impaired cell migration in in vitro wound healing assays and live cell tracking experiments. This was likely due to a shift in the adhesion-contractility balance during cell migration. TNCEGFL expressing cells presented a spindle-shaped morphology and formed cords in culture. Expression of TNCEGFL delayed melanoma cell attachment and spreading, as determined in cell adhesion and inverted centrifugation assays. This anti-adhesive phenotype was concomitant with increased MLC2 phosphorylation. Inhibition of ROCK activity, which drives transcellular contractility, restored adhesion of TNCEGFL expressing cells and improved migration in 2D. In distinction to the findings in 2D, TNCEGFL expressing cells had higher invasion potential in Matrigel invasion assays, with cells expressing high TNC having rounded “ameboid” morphology, underlying the importance of morphological shifts, and possibly ROCK signaling, in 3D movement through barrier matrices during invasion and dissemination. These results imply that TNCEGFL repeats are involved in melanoma invasion both by directly influencing cell migration and indirectly changing the tumor microenvironment composition, possibly allowing transition from mesenchymal to ameboid migrational mode. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 485. doi:1538-7445.AM2012-485
Role of chronic ryanodine receptor phosphorylation in heart failure and β-adrenergic receptor blockade in mice
Authors: Jian Shan, Matthew J. Betzenhauser, Alexander Kushnir, Steven Reiken, Albano C. Meli, Anetta Wronska, Miroslav Dura, Bi-Xing Chen, Andrew R. Marks
Journal: Journal of Clinical Investigation (2010)

Increased sarcoplasmic reticulum (SR) Ca2+ leak via the cardiac ryanodine receptor/calcium release channel (RyR2) is thought to play a role in heart failure (HF) progression. Inhibition of this leak is an emerging therapeutic strategy. To explore the role of chronic PKA phosphorylation of RyR2 in HF pathogenesis and treatment, we generated a knockin mouse with aspartic acid replacing serine 2808 (mice are referred to herein as RyR2-S2808D+/+ mice). This mutation mimics constitutive PKA hyperphosphorylation of RyR2, which causes depletion of the stabilizing subunit FKBP12.6 (also known as calstabin2), resulting in leaky RyR2. RyR2-S2808D+/+ mice developed age-dependent cardiomyopathy, elevated RyR2 oxidation and nitrosylation, reduced SR Ca2+ store content, and increased diastolic SR Ca2+ leak. After myocardial infarction, RyR2-S2808D+/+ mice exhibited increased mortality compared with WT littermates. Treatment with S107, a 1,4-benzothiazepine derivative that stabilizes RyR2-calstabin2 interactions, inhibited the RyR2-mediated diastolic SR Ca2+ leak and reduced HF progression in WT and RyR2-S2808D+/+ mice. In contrast, β-adrenergic receptor blockers improved cardiac function in WT but not in RyR2-S2808D+/+ mice.Thus, chronic PKA hyperphosphorylation of RyR2 results in a diastolic leak that causes cardiac dysfunction. Reversing PKA hyperphosphorylation of RyR2 is an important mechanism underlying the therapeutic action of β-blocker therapy in HF.
Photochemical Finish: Assessing the Genomic Impact of Secondary Air Pollutants
Authors: Bob Weinhold
Journal: Environmental Health Perspectives (2011)

The Relationship between Maternal Stress and Offspring DiseaseStudies have suggested a potential association between maternal stress during pregnancy and an increased risk of specific diseases in offspring, prompting calls for a closer examination of stress as an environmental health factor.A new comprehensive study based on a large population-based cohort in Denmark explores this association further by examining maternal stress during pregnancy and the development of a wide spectrum of pediatric diseases [EHP 119(11):1647-1652; Tegethoff et al.].The researchers examined prospective data for 66,203 mother-child pairs enrolled in the Danish National Birth Cohort.The mothers gave birth between 1996 and 2003, and the median child age at the end of followup was 6.2 years.The researchers assessed two types of stress during each woman's pregnancy-"life stress" and "emotional stress"-based on information reported by mothers at about 30 weeks' gestation.Life stress refers to the mother's perceived life burdens, e.g., work, housing, and human relations.Emotional stress refers to the mother's feelings, e.g., irrational fear, hopelessness, and irritation.The researchers also collected data on disease diagnoses reported to the Danish National Hospital Register for the study children.
Review Body's Report: Implementing the Charter
Authors: K. H. Gillison
Journal: BMJ (1966)

SIR,-While not disputing that all doctors deserve an increase in their remuneration, it is surely quite incredible that a senior regis- trar and consultant, after many years of highly competitive and specialized training involving great personal sacrifice in time and money, and of family and home, constantly on the move and without any security what- ever, should now reap an ultimate reward less than that of the family doctor.This surely is the ultimate negation of personal effort to achieve a specialist position, and the opposite of any premise in industry, com- merce, or any other profession.That a one- year house-surgeon can now go into general practice and earn more than his own hospital chief, a man who took about 15 years to reach this position, must surely mean by even the simplest logic that the death knell of future hospital services has been sounded loud and clear.-Iam, etc., London N. 10.
Fibrinogen: a possible link between social class and coronary heart disease.
Authors: H L Markowe, M G Marmot, M J Shipley, C J Bulpitt, T W Meade, Y Stirling, M V Vickers, A Semmence
Journal: BMJ (1985)

Mortality from coronary heart disease in civil servants in the lowest grade of employment has been found to be about three times that of men in the highest grade of employment. As part of an investigation of this finding several haemostatic variables were measured in a sample of 29 men in lower grades of employment and 45 men in higher grades. There was a significant difference in plasma fibrinogen concentrations between men in lower grades of employment and those in higher grades (mean 3.39 g/l v 2.95 g/l, respectively; p less than 0.01) but not in other haemostatic variables. Multiple regression analyses showed significant independent associations of fibrinogen concentration with smoking (p less than 0.05) and grade of employment (p less than 0.05). The size of the observed difference between the grades of employment was similar to that between those dying of coronary heart disease or surviving during longitudinal study; it may therefore be an important part of the mechanism underlying social class differences in coronary heart disease. The statistical relation between fibrinogen concentrations and other characteristics that may be concerned in the aetiology of coronary heart disease was examined. A summary measure of job stress was significantly related to fibrinogen concentration (p less than 0.01) and made a substantial contribution to explaining the differences between grades of employment. Behaviour type and a score of physical activity were not significantly related to fibrinogen concentration.
Predicting the Outcome From Hypoxic-Ischemic Coma: Ethical Implications-Reply
Authors: Peter McL. Black
Journal: JAMA (1986)

<h3>In Reply.—</h3> I am grateful to Dr Brody for correcting any impression I may have left that consequentialist analysis is necessary to withhold treatment in comatose patients. Nonconsequentialist analysis can certainly lead to the same conclusion, for example by deciding that the irreversibly comatose patient is not a "person." Consequentialist analysis is, however, implicit in the article by Levy et al<sup>1</sup>that was under discussion. The fundamental ethical stance of the President's Commission<sup>2</sup>may or may not be consequentialist; their claim that continued maintenance of a permanently unconscious patient can constitute "an irresponsible stewardship of society's resources" (p186) appears to rest on consequentialist cost-benefit considerations. I do not find convincing support for the preconditions for their analysis that Dr Brody mentions in his penultimate sentence; moreover, the "incompetent but noncomatose" patient is not the patient under consideration here. Consequentialist analysis is not necessarily bad and nonconsequentialist analysis is
The Sex-Conversion Controversy
Authors: Donald Hayes Russell
Journal: New England Journal of Medicine (1968)

THE surgical treatment of the conditions of hermaphrodism and pseudohermaphrodism1 to correct nature's mistakes, that the sexual identity and function of such persons may be established, has long been accepted as a contribution of medical science to suffering mankind. Such procedures are constructive in intent, usually do not destroy the patient's procreative ability and do not differ in the legal, moral and ethical considerations involved generally in surgery. More recently, especially since the highly publicized Jorgensen case in 1952, the issue of medical and surgical intervention to affect a change of external sexual characteristics in persons so desiring, but who . . .
Isolated chemotherapeutic perfusion as neoadjuvant therapy for advanced/unresectable plevic malignancy.
Authors: Harold J. Wanebo, Michael Ross DiSiena, James Belliveau, Eric Gustafson
Journal: Journal of Clinical Oncology (2012)

e13561 Background: Isolated pelvic perfusion (IPP) may have value in therapy of recurrent pelvic malignancy following previous surgery and chemoradiation. We performed 113 IPP in 78 such pts (34 palliation, and 44 pre op) using a simplified balloon occlusion technique. Methods: Of 42 patients (pts) with recurrent rectal cancer (ca), 26 had preop and 16 had palliative IPP. Other pelvic cancers included anal canal (8 pts), pelvic sarcoma (5 pts), melanoma (M) (4 pts), endometrial ca (EC) 2 pts, ovarian ca (OC) 2 pts, and bladder ca (BC) 1 pt. Chemo agents included Paclitaxel, 5FU, cisplatinum, or Oxaliplatin and mitomycin for epithelial cancer and (Doxorubicin, Ifosamide, Phenyl Alanine Mustard (PAM) for remaining tumors. High dose IPP with PAM, Paclitaxel and Cisplatin was given in 6 pts, 3 with stem cell support. Results: Palliative IPP in advanced rectal cancer (AdRca) pts relieved narcotic resistant pain (2-4 mos) in 11/16 pts (69%). Preop IPP in 26 AdRca achieved path CR in 2 pts and partial regression in 11 pts; 7 had RO resection. Of 5 other pts, 3 refused resection, 2 were inoperable. Median survival was 17 mos in 12 resectable pts and 30 mos in 7 resected pts and 8 mos in 12 non resectable pts. It was 30 months in 8 pts with anorectal ca (1&gt;90 mos), 20 mos in 4 endometrial/ovarian ca pts, (1 died NED &gt;48 mos), 13 mos in 4 M pts and 5 (4-34) mos in 5 pelvic sarcoma pts. Overall 17 of 44 (39%) were resected and 24 were palliated with IPP. Conclusions: IPP has value in palliating or augmenting resectability and survival in advanced pelvic cancer patients not amenable to conventional chemoradiation and surgery.
Tropical dermatology.
Authors: A. Bryceson
Journal: British Journal of Dermatology (1976)

Journal Article Tropical dermatology: CUTANEOUS LEISHMANIASIS Get access A. Bryceson A. Bryceson Hospital for Tropical Diseases, 4 St Pancras Way, London NW1 oPE Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 94, Issue 2, 1 February 1976, Pages 223–226, https://doi.org/10.1111/j.1365-2133.1976.tb04375.x Published: 01 February 1976