tbuckley/cabot-search huggingface dataset:
Abstract 3515: Gefitinib-resistant cell line derived from PC-9 has high sensitivity to SN-38
Authors: Asao Sakai, Hideharu Kimura, Takashi Sone, Tohru Ohmori, Kazuo Kasahara, Masaki Fujimura
Journal: Cancer Research (2010)

Abstract Most non-small cell lung cancer (NSCLC) tumors with EGFR mutations are responsive to EGFR tyrosine kinase inhibitors such as gefitinib. Preliminary experiments with PC-9/MET, a gefitinib resistant cell line derived from PC-9 showed a higher sensitivity to SN-38, which is an active metabolite of CPT-11, in comparison to parental PC-9. The mechanisms of the collateral sensitivity to SN-38 were investigated in the resistant cell line. The WST-1 assay showed that PC-9/MET was more sensitive, with an IC50 of 0.027 ± 0.014 μM, to SN-38 than that to PC-9 with an IC50 of 0.093 ± 0.049 μM. Topoisomerase-I (Topo-I) mRNA expression value in PC-9/MET to increase 9.2 times in comparison to that of PC-9. The Topo-I activity of PC-9/MET was higher than that of PC-9 and the Topo-I protein expression was also higher in PC-9/MET. PC-9/MET has a greater expression of MET in comparison to PC-9. The suppression of MET expression by an MET-specific siRNA resulted in a decrease in the Topo-I activity and Topo-I protein expression in PC-9/MET. In conclusion, the Topo-I activity and protein expression in PC-9/MET is elevated via the mRNA expression, thus resulting in collateral sensitivity to SN-38. The MET over-expression in PC-9/MET may therefore be one of the mechanisms which cause an increase in the Topo-I activity and expression. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3515.
Whipple's disease presenting with symmetrical panniculitis
Authors: A.C. Friedmann, G.K. Perera, A. Jayaprakasam, I. Forgacs, J.R. Salisbury, D. Creamer
Journal: British Journal of Dermatology (2004)

Specific cutaneous involvement in Whipple's disease is extremely rare. The condition usually runs a chronic course, with symptoms preceding diagnosis by years or even decades. We report a 44-year-old man who presented with a rapid onset of progressive, extensive, symmetrical plaques of panniculitis affecting the inner thighs and forearms. He had accompanying large joint arthritis and was profoundly anaemic. Biopsy of the subcutis revealed a florid septal panniculitis with infiltration of the septa by foamy macrophages containing intracellular granules that stained strongly with periodic acid-Schiff reagent. A similar but more intense infiltrate was seen in the small bowel lamina propria, and a diagnosis of Whipple's disease was made. Symmetrical panniculitis has not previously been reported as a manifestation of Whipple's disease.
PERSPIRATION AND THE ELECTRIC FAN
Authors: J. F. Baldwin
Journal: JAMA (1925)

To the Editor:  —InThe Journal, October 31, page 1420, a correspondent asks as to the employment of an electric fan for the surgeon in the operating room who is given to perspiration. His question is partially answered by Kirschner, on page 1439 of the same issue. He distinctly advises the use of electric fans to play on the operating staff above the table.  For a good many years I have used in the operating room a pretty fair sized electric fan, tilted at such an angle that the breeze strikes the head and shoulders of myself and my assistants, but is above the level of the patient. The experience has been in every way exceedingly satisfactory, as such a fan adds materially to the comfort and well being of those within reach. There has not been the slightest evidence of any ill effect on the patients.
Drosophila Neuroligin 1 Promotes Growth and Postsynaptic Differentiation at Glutamatergic Neuromuscular Junctions
Authors: Daniel Banovic, Omid Khorramshahi, David Owald, Carolin Wichmann, Tamara Riedt, Wernher Fouquet, Rui Tian, Stephan J. Sigrist, Hermann Aberle
Journal: Neuron (2010)
Adriamycin and the Heart
Authors: I. Craig Henderson, Emil Frei
Journal: New England Journal of Medicine (1979)

Doxorubicin, or adriamycin, is one of the most effective chemotherapeutic agents for cancer. It has an established role in the treatment of the acute leukemias and the lymphomas. In contrast to most other drugs, it has substantial activity in solid tumors as well. It is the single most effective agent in the treatment of breast cancer and soft-tissue sarcomas, and it has substantial activity in the childhood tumors, testicular cancer, gastric cancer, ovarian cancer and oat-cell carcinoma of the lung.1 The acute dose-limiting toxicity of this drug, as with most chemotherapeutic agents, is myelosuppression. Other side effects, including nausea, vomiting . . .
Antigen-induced monocyte procoagulant activity. Requirement for antigen presentation and histocompatibility leukocyte antigen-DR molecules.
Authors: B S Schwartz
Journal: Journal of Clinical Investigation (1985)

The present study explores the interactions between lymphocytes and monocytes that are required for expression of procoagulant activity (PCA) by monocytes in response to purified protein derivative of the tubercle bacillus (PPD) or tularemia antigen. The PCA response was antigen specific: peripheral blood mononuclear cells (PBM) from donors sensitive to PPD or tularemia showed an increase in PCA only in response to the sensitizing antigen. The PCA was tissue factorlike in that Factors VII and X were required for expression of the activity, whereas Factor VIII was not. Maximum PCA developed only after 36 to 72 h. Fractionation of PBM into lymphocytes and monocytes after antigenic stimulation demonstrated that greater than 90% of the PCA was associated with monocytes. Isolated monocytes or lymphocytes incubated with sensitizing antigen had the same PCA as control cells. Purified lymphocytes that had been pulsed with antigen were unable to elicit a PCA response from monocytes to which they were added. However, adherent monocytes incubated with antigen, then washed free of unbound protein, were able to trigger lymphocytes to become stimulatory for PCA toward responding monocytes. The development of antigen-specific PCA in PBM could be blocked by including a monoclonal antibody to HLA-DR antigen in the incubation. The antibody had no effect on the clotting assay, on preformed PCA, cell viability, or on stimulatory antigen itself. These results indicate that elaboration of PCA by mononuclear cells may be an intrinsic part of the classical immune response to antigen, and may explain the presence of fibrin in immune lesions, as well as the occurrence of thrombotic complications in many immune disorders.
Head of German medicines body likens HRT to thalidomide
Authors: J. Burgermeister
Journal: BMJ (2003)

Doctors in Germany should drastically reduce the amount of hormone replacement therapy (HRT) that they prescribe to older women, a key commission has recommended.

Issuing its first guidelines on HRT to doctors in Germany, the country's Commission on the Safety of Medicines said that new international studies had shown that the risks of HRT clearly outweighed the benefits.

It recommended that doctors offer the treatment only to women with particularly severe menopausal symptoms. It also recommended that treatment …
Randomised trial of an inhaled beta 2 agonist, inhaled corticosteroid and their combination in the treatment of asthma
Authors: R J Hancox, J O Cowan, E M Flannery, G P Herbison, C R McLachlan, C S Wong, D R Taylor
Journal: Thorax (1999)

<h3>BACKGROUND</h3> Although many asthmatic patients are treated with a combination of β<sub>2</sub> agonist and corticosteroid inhalers, the clinical effects of combining the drugs are unknown. Studies on the early asthmatic response to allergen suggest that β<sub>2</sub> agonists may reduce the benefit of inhaled corticosteroids. A study of the effects of combining the drugs on asthma control was undertaken. <h3>METHODS</h3> Sixty one subjects with mild to moderate asthma were randomised to a double blind crossover comparison of inhaled budesonide (200–400 μg twice daily), terbutaline (500–1000 μg four times daily), combined treatment, and placebo. Each treatment was given for six weeks following a four week washout period. Ipratropium was used for symptom relief. Treatments were ranked from worst (1) to best (4) based on need for oral steroid, mean morning peak flow, nocturnal awakening, ipratropium use, and asthma symptoms. Lung function and bronchial hyperresponsiveness were measured before and after each treatment. <h3>RESULTS</h3> Evaluable data for all four treatments were obtained from 47 subjects. The mean rank of each treatment was: placebo = 2.05; terbutaline = 2.13; budesonide = 2.48; combined treatment = 3.34. Combined treatment was ranked significantly better than any other treatment (p&lt;0.01). Mean (95% CI) morning and evening peak flows were 14 (5 to 23) and 24 (15 to 34) l/min higher, respectively, during combined treatment than during budesonide, and 27 (17 to 37) and 15 (7 to 23) l/min higher than during terbutaline. Asthma symptoms tended to be least frequent during combined treatment but were not significantly different from budesonide alone. There was no significant difference between combined treatment and budesonide alone for lung function and bronchial hyperresponsiveness. <h3>CONCLUSIONS</h3> In this group of mild to moderate asthmatic subjects the combination of β<sub>2</sub> agonist and corticosteroid gave better asthma control than either treatment alone. There was no evidence that regular β<sub>2</sub> agonist treatment impaired the beneficial effect of inhaled corticosteroid.
CARDIOMYOPATHY IN ACUTE ISCHEMIC STROKE
Authors: Gavin Hickey, Omar Batal, Nadeem Kolia, Sandeep Patel, Bhavna Balaney, Zeina Dardari, Vivek Reddy, Tudor Jovin, Maxim Hammer, Mark Schmidhofer, Hunter Champion
Journal: Journal of the American College of Cardiology (2013)
Endoscopic Mucosal Resection in the Stomach Using the Insulated-Tip Needle-Knife
Authors: M. Muto, S. Miyamoto, A. Hosokawa, T. Doi, A. Ohtsu, S. Yoshida, Y. Endo, K. Hosokawa, D. Saito, C.-S. Shim, L. Gossner
Journal: Endoscopy (2005)

Endoscopic mucosal resection (EMR) is now accepted as a standard treatment for intramucosal gastric cancer in Japan. Since Tada et al. described the strip biopsy method in 1983 [1], this technique has been widely used not only in Japan but also in other countries and has been improved in a variety of ways [2]. According to the guidelines for the treatment of gastric cancer from by the Japanese Gastric Cancer Association, the indications for EMR are: intramucosal cancer; a diameter of 20 mm or less; intestinal type, histologically; and no findings of ulcer. However, with conventional techniques such as the strip biopsy method, it is difficult to obtain a large specimen as a "one-piece" or "en bloc" resection.
Fluoxetine Versus Placebo in Advanced Cancer Outpatients: A Double-Blinded Trial of the Hoosier Oncology Group
Authors: Michael J. Fisch, Patrick J. Loehrer, Jean Kristeller, Steven Passik, Sin-Ho Jung, Jianzhao Shen, Matthew A. Arquette, Mary J. Brames, Lawrence H. Einhorn
Journal: Journal of Clinical Oncology (2003)

To determine whether fluoxetine improves overall quality of life (QOL) in advanced cancer patients with symptoms of depression revealed by a simple survey.One hundred sixty-three patients with an advanced solid tumor and expected survival between 3 and 24 months were randomly assigned in a double-blinded fashion to receive either fluoxetine (20 mg daily) or placebo for 12 weeks. Patients were screened for at least minimal depressive symptoms and assessed every 3 to 6 weeks for QOL and depression. Patients with recent exposure to antidepressants were excluded.The groups were comparable at baseline in terms of age, sex, disease distribution, performance status, and level of depressive symptoms. One hundred twenty-nine patients (79%) completed at least one follow-up assessment. Analysis using generalized estimating equation modeling revealed that patients treated with fluoxetine exhibited a significant improvement in QOL as shown by the Functional Assessment of Cancer Therapy-General, compared with patients given placebo (P =.01). Specifically, the level of depressive symptoms expressed was lower in patients treated with fluoxetine (P =.0005), and the subgroup of patients showing higher levels of depressive symptoms on the two-question screening survey were the most likely to benefit from treatment.In this mix of patients with advanced cancer who had symptoms of depression as determined by a two-question bedside survey, use of fluoxetine was well tolerated, overall QOL was improved, and depressive symptoms were reduced.
Can dermopscopy improve the poor reliability of clinical approach in the diagnosis of lentigo maligna of the face?
Authors: M. Stante, V. De Giorgi, P. Carli
Journal: British Journal of Dermatology (2005)

Journal Article Can dermopscopy improve the poor reliability of clinical approach in the diagnosis of lentigo maligna of the face? Get access M. Stante, M. Stante Pigmented Lesions Clinic, Department of Dermatology, University of Florence, Via della Pergola 58–60, 50121 Florence Italy E‐mail: drstante@aliceposta.it Search for other works by this author on: Oxford Academic Google Scholar V. De Giorgi, V. De Giorgi Pigmented Lesions Clinic, Department of Dermatology, University of Florence, Via della Pergola 58–60, 50121 Florence Italy E‐mail: drstante@aliceposta.it Search for other works by this author on: Oxford Academic Google Scholar P. Carli P. Carli Pigmented Lesions Clinic, Department of Dermatology, University of Florence, Via della Pergola 58–60, 50121 Florence Italy E‐mail: drstante@aliceposta.it Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 153, Issue 5, 1 November 2005, Page 1084, https://doi.org/10.1111/j.1365-2133.2005.06927.x Published: 01 November 2005
The kidney deficit: a lingering problem
Authors: Elizabeth Rasche González
Journal: JAMA (1980)

Neither hemodialysis nor renal transplantation is any bed of roses for the patient.  However, transplantation enables more patients with  disease to return to full employment and normal lives. Moreover, after a critical postoperative period of 18 to 24 months, a successful transplant group is less expensive than a dialysis group—a salient fact given the estimated $1.5 billion bill that the federal government will pick up this year for dialysis and transplantation.  But some patients on waiting lists never get suitable donor kidneys. Although hard data concerning the kidney deficit are impossible to come by, Steven Teutsch, MD, MPH, chief, Kidney Donor Activity, Center for Disease Control (CDC), Atlanta, believes that a 60% increase in available kidneys in the United States—from the 3,000 per year that are currently transplanted—would probably take care of all the presumed potential recipients.  In addition, Teutsch toldJAMAMEDICAL NEWS, the results of renal transplantation
Detection of autonomic sympathetic dysfunction in diabetic patients. A study using laser Doppler imaging.
Authors: S Bornmyr, J Castenfors, H Svensson, M Wroblewski, G Sundkvist, P Wollmer
Journal: Diabetes Care (1999)

OBJECTIVE: To study signs of the disturbed reflex autonomic sympathetic nerve function in type 1 and type 2 diabetic patients. RESEARCH DESIGN AND METHODS: Measurements were made on 15 type 1 (duration 13-32 years) and on 50 recently diagnosed type 2 diabetic patients (duration 3-4 years). The vasoconstrictor responses in the distal phalanx of the middle finger (locally heated to 40 degrees C) to the cooling of the contralateral arm were measured using Laser Doppler Imaging (LDI). A vasoconstriction index (VAC) was calculated taking age into account and was compared with reference values obtained in 80 control subjects. The diabetic patients were also studied with deep-breathing tests (i.e., the heart-rate variation expressed as the expiration-to-inspiration [E/I] ratio, a test of parasympathetic nerve function). RESULTS: The vasoconstrictor responses to indirect cooling (VAC) were significantly reduced in the fingers of the diabetic patients, both type 2 (0.77 +/- 0.02 V; P &amp;lt; 0.01) and type 1 (0.83 +/- 0.04 V; P &amp;lt; 0.001), compared with the healthy control subjects (0.65 +/- 0.01); the age-corrected VAC (VACz) was slightly more impaired in type 1 than in type 2 diabetic patients. The frequency of an abnormal VACz corresponded well to the frequency of an abnormal E/I ratio in type 1 diabetic patients (approximately 50%), whereas the frequency of an abnormal VACz was significantly higher than an abnormal E/I ratio among type 2 diabetic patients (11/50 vs. 4/50; P &amp;lt; 0.05). CONCLUSIONS: Both type 1 and type 2 diabetic patients have impaired cutaneous blood flow regulation. The VAC index seems to be a promising tool for detection of subclinical changes in autonomic sympathetic function.
Recommendations on the Use of Botulinum Toxin in the Treatment of Lower Urinary Tract Disorders and Pelvic Floor Dysfunctions: A European Consensus Report
Authors: Apostolos Apostolidis, Prokar Dasgupta, Pierre Denys, Sohier Elneil, Clare J. Fowler, Antonella Giannantoni, Gilles Karsenty, Heinrich Schulte-Baukloh, Brigitte Schurch, Jean-Jacques Wyndaele
Journal: European Urology (2008)
Interleukin-7 and interleukin-15 regulate the expression of thebcl-2 and c-myb genes in cutaneous T-cell lymphoma cells
Authors: Jian-Zhong Qin, Chun-Lei Zhang, Jivko Kamarashev, Reinhard Dummer, Günter Burg, Udo Döbbeling
Journal: Blood (2001)
Membrane cofactor protein mutations in atypical hemolytic uremic syndrome (aHUS), fatal Stx-HUS, C3 glomerulonephritis, and the HELLP syndrome
Authors: Celia J. Fang, Veronique Fremeaux-Bacchi, M. Kathryn Liszewski, Gaia Pianetti, Marina Noris, Timothy H. J. Goodship, John P. Atkinson
Journal: Blood (2007)
Ultrasound Imaging of Acute Cardiac Transplant Rejection With Microbubbles Targeted to Intercellular Adhesion Molecule-1
Authors: Gregory E.R. Weller, Erxiong Lu, Melissa M. Csikari, Alexander L. Klibanov, David Fischer, William R. Wagner, Flordeliza S. Villanueva
Journal: Circulation (2003)

Noninvasive techniques for detecting acute cardiac transplant rejection are limited. We hypothesized that ultrasound contrast microbubbles targeted to the endothelial cell (EC) inflammatory marker intercellular adhesion molecule-1 (ICAM-1) would selectively bind to rejecting versus nonrejecting myocardium and that myocardial contrast echocardiography can therefore detect acute rejection.Lipid-based microbubbles were conjugated to anti-rat ICAM-1 (MBICAM) or isotype control antibody (MBControl). In vitro MBICAM adhesion to cultured rat ECs, as assessed in a parallel plate flow apparatus, was greater to inflammatory versus normal ECs (11+/-3 versus 3+/-2 microbubbles/EC, P<0.005). In vivo abdominal heterotopic heart transplantation was performed in rats (rejection group: Brown Norway to Lewis strain; control group: Lewis to Lewis or Brown Norway to Brown Norway). Triggered myocardial contrast echocardiography was performed during intravenous MBICAM or MBControl (2.5x10(6)) injection on postoperative day 5. Myocardial videointensity from adhered MBICAM was significantly higher in rejecting (n=8) versus control (n=7) rats (10+/-4 versus 1+/-4 U, P=0.01). Postmortem histology showed normal myocardium in control rats, whereas allograft myocardium demonstrated grade III to IV rejection and strong immunohistochemical ICAM-1 staining.Preferential adherence of ICAM-1-targeted microbubbles to rejecting versus nonrejecting rat cardiac transplant myocardium can be detected ultrasonically. Targeted microbubbles may thus offer a noninvasive ultrasound imaging technique for the detection of acute cardiac transplant rejection and other processes characterized by endothelial dysfunction.
Thrombocytopenia Caused by Abciximab or Tirofiban and Its Association With Clinical Outcome in Patients Undergoing Coronary Stenting
Authors: Piera Angelica Merlini, Marco Rossi, Alberto Menozzi, Silvia Buratti, Danielle M. Brennan, David J. Moliterno, Eric J. Topol, Diego Ardissino
Journal: Circulation (2004)

Thrombocytopenia is a possible complication of treatment with glycoprotein (GP) IIb/IIIa antagonists during percutaneous coronary interventions, but it is not clear whether different GP IIb/IIIa inhibitors carry a different risk of thrombocytopenia, and its relation to clinical outcome is unknown.We analyzed data from the Do Tirofiban and Reopro Give Similar Efficacy Outcomes (TARGET) study, which compared the safety and efficacy of abciximab and tirofiban in patients undergoing coronary stenting. Platelets were measured at baseline and 6 and 24 hours after the beginning of treatment. Thrombocytopenia (nadir platelet count <100x10(9) cells/L) developed in 2.4% of patients treated with abciximab and 0.5% of those treated with tirofiban (P<0.001). The variables independently associated with thrombocytopenia were treatment with abciximab within the previous 6 months (OR, 4.4; 95% CI, 1.7 to 11.2), baseline creatinine levels of > or =0.8 mg/dL (OR, 3.8; 95% CI, 1.7 to 8.8), previous transient ischemic attack (OR, 3.2; 95% CI, 1.4 to 7.6), female gender (OR, 1.9; 95% CI, 1.2 to 3.1), and history of peripheral vascular disease (OR, 1.78; 95% CI, 1.0 to 3.1). Severe bleeding occurred more frequently in patients with thrombocytopenia (5.1% versus 0.7%, P=0.001), who also more frequently received blood transfusions (6.1% versus 1.4%, P=0.001). At the 30-day follow-up, 2.0% of patients with thrombocytopenia and 0.4% of those without (P=0.022) had died; myocardial infarction occurred in 9.13% versus 6.11% (P=NS); and target vessel revascularization occurred in 6.07% versus 0.60% (P<0.001).During coronary stenting, abciximab and other risk factors are independently associated with thrombocytopenia. Regardless of the cause, thrombocytopenia is associated with more ischemic events, bleedings, and transfusions.
Comparative estrogenic activity of wine extracts and organochlorine pesticide residues in food.
Authors: K Gaido, L Dohme, F Wang, I Chen, B Blankvoort, K Ramamoorthy, S Safe
Journal: Environmental Health Perspectives (1998)

The human diet contains industrial-derived, endocrine-active chemicals and higher levels of naturally occurring compounds that modulate multiple endocrine pathways. Hazard and risk assessment of these mixtures is complicated by noadditive interactions between different endocrine-mediated responses. This study focused on estrogenic chemicals in the diet and compared the relative potencies or estrogen equivalents (EQs) of the daily consumption of xenoestrogenic organochlorine pesticides in food (2.44 micrograms/day) with the EQs in a single 200-ml glass of red cabernet wine. The reconstituted organochlorine mixture contained 1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl)ethane, 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane, 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene, endosulfan-1, endosulfan-2, p,p'-methoxychlor, and toxaphene; the relative proportion of each chemical in the mixture resembled the composition reported in a recent U.S. Food and Drug Administration market basket survey. The following battery of in vitro 17 beta-estradiol (E2)-responsive bioassays were utilized in this study: competitive binding to mouse uterine estrogen receptor (ER); proliferation in T47D human breast cancer cells; luciferase (Luc) induction in human HepG2 cells transiently cotransfected with C3-Luc and the human ER, rat ER-alpha, or rat ER-beta; induction of chloramphenicol acetyltransferase (CAT) activity in MCF-7 human breast cancer cells transfected with E2-responsive cathepsin D-CAT or creatine kinase B-CAT plasmids. For these seven in vitro assays, the calculated EQs in extracts from 200 ml of red cabernet wine varied from 0.15 to 3.68 micrograms/day. In contrast, EQs for consumption of organochlorine pesticides (2.44 micrograms/day) varied from nondetectable to 1.24 ng/day. Based on results of the in vitro bioassays, organochlorine pesticides in food contribute minimally to dietary EQ intake.