tbuckley/cabot-search huggingface dataset:
Frequent rearrangements of retinoic acid receptor alpha gene and myl gene, and rare mutations of RAS and FMS genes in acute promyelocytic leukemia
Authors: Koh Yamamoto, Shinsaku Hirosawa, Hisashi Sakamaki, Nobuo Aoki
Journal: American Journal of Hematology (1992)

To investigate leukemogenesis of acute promyelocytic leukemia (APL), we studied the involvements of retinoic acid receptor alpha (RAR alpha) and myl genes, and also the frequency of N-RAS, K-RAS, H-RAS, and FMS point mutations in sixteen patients with APL. By Southern blot analysis, the rearrangements of RAR alpha gene were detected in 13 patients (81.2%), and myl gene in 14 (87.5%). Either RAR alpha or myl gene rearrangements were found in all patients including one with normal karyotype. Breakpoints of both genes were clustered. By direct sequencing, no point mutations were found at codons 12, 13, and 61 of N-, K-, and H-RAS genes, and at codons 301 and 969 of FMS gene. These data indicate that myl-RAR alpha translocation occurs frequently in APL, whereas RAS and FMS mutations are rare in APL. It may be suggested that leukemogenesis of APL is different from other subtypes of acute myelogenous leukemia, and multistep leukemogenesis may not be a prevalent feature in APL.
AN ECONOMICAL COVER FOR LABORATORY INSTRUMENTS
Authors: R. H. McClellan
Journal: JAMA (1933)

In localities in which there is a large amount of dust in the air, a protective covering for such instruments as the microscope, colorimeter and microtome becomes a necessity. The usual glass bell jar is heavy, awkward, expensive and easily broken. It is in the hope that others will find it advantageous to use that a description of a cover I have devised is presented in the accompanying illustrations. Discarded noninflammable roentgenograms are placed in warm solution of sodium bicarbonate and the photosensitive film is removed, care being taken not to scratch the base. Accurate measurements of the proposed covers are made and a design worked out on paper, so as to utilize the size of film on hand and to make as few joints as possible. The film is ruled with a glass marking pencil and cut to size. At the joints it is necessary to provide for a
Renin angiotensin system and gender differences in dopaminergic degeneration
Authors: Ana I Rodriguez-Perez, Rita Valenzuela, Belen Joglar, Pablo Garrido-Gil, Maria J Guerra, Jose L Labandeira-Garcia
Journal: Molecular Neurodegeneration (2011)

Abstract Background There are sex differences in dopaminergic degeneration. Men are approximately two times as likely as premenopausal women of the same age to develop Parkinson's disease (PD). It has been shown that the local renin angiotensin system (RAS) plays a prominent role in sex differences in the development of chronic renal and cardiovascular diseases, and there is a local RAS in the substantia nigra and dopaminergic cell loss is enhanced by angiotensin via type 1 (AT1) receptors. Results In the present study, we observed that intrastriatal injection of 6-hydroxydopamine induced a marked loss of dopaminergic neurons in the substantia nigra of male rats, which was significantly higher than the loss induced in ovariectomized female rats given estrogen implants (i.e. rats with estrogen). However, the loss of dopaminergic neurons was significantly lower in male rats treated with the AT1 antagonist candesartan, and similar to that observed in female rats with estrogen. The involvement of the RAS in gender differences in dopaminergic degeneration was confirmed with AT1a-null mice lesioned with the dopaminergic neurotoxin MPTP. Significantly higher expression of AT1 receptors, angiotensin converting enzyme activity, and NADPH-oxidase complex activity, and much lower levels of AT2 receptors were observed in male rats than in female rats with estrogen. Conclusions The results suggest that brain RAS plays a major role in the increased risk of developing PD in men, and that manipulation of brain RAS may be an efficient approach for neuroprotective treatment of PD in men, without the feminizing effects of estrogen.
High-Density Lipoprotein and Dietary Fatty Acids
Authors: Dae Hyun Kim
Journal: JAMA (2007)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
CHOICE OF A TIME OF ELECTION IN MASTOID OPERATIONS.SOME CONSIDERATIONS ARISING FROM THE DIFFICULTIES OF THE CHOICE—PROSPECTIVE RESULTS.
Authors: D. A. KUYK
Journal: JAMA (1905)

tion into the bowel of air or water, when properly done, is sometimes followed by reduction, and Dr. Kelley does not think the method should be altogether dispensed with.Very little is said in regard to the way in which it should be applied, the amoLnt of pressure that should be used, nor the length of time the pressure should be maintained.Dr. Kelley thinks the figures that Morris gave some years ago placed the pressure at 8 or 9 pounds, which appears to be too high.He believes that air pressure is preferable to water pressure in very many cases, but it is so difficult to measure that he finds it succeeds
Maple syrup urine disease. Complete primary structure of the E1 beta subunit of human branched chain alpha-ketoacid dehydrogenase complex deduced from the nucleotide sequence and a gene analysis of patients with this disease.
Authors: Y Nobukuni, H Mitsubuchi, F Endo, I Akaboshi, J Asaka, I Matsuda
Journal: Journal of Clinical Investigation (1990)

A defect in the E1 beta subunit of the branched chain alpha-ketoacid dehydrogenase (BCKDH) complex is one cause of maple syrup urine disease (MSUD). In an attempt to elucidate the molecular basis of MSUD, we isolated and characterized a 1.35 kbp cDNA clone encoding the entire precursor of the E1 beta subunit of BCKDH complex from a human placental cDNA library. Nucleotide sequence analysis revealed that the isolated cDNA clone (lambda hBE1 beta-1) contained a 5'-untranslated sequence of four nucleotides, the translated sequence of 1,176 nucleotides and the 3'-untranslated sequence of 169 nucleotides. Comparison of the amino acid sequence predicted from the nucleotide sequence of the cDNA insert of the clone with the NH2-terminal amino acid sequence of the purified mature bovine BCKDH-E1 beta subunit showed that the cDNA insert encodes for a 342-amino acid subunit with a Mr = 37,585. The subunit is synthesized as the precursor with a leader sequence of 50 amino acids and is processed at the NH2 terminus. A search for protein homology revealed that the primary structure of human BCKDH-E1 beta was similar to the bovine BCKDH-E1 beta and to the E1 beta subunit of human pyruvate dehydrogenase complex, in all regions. The structures and functions of mammalian alpha-ketoacid dehydrogenase complexes are apparently highly conserved. Genomic DNA from lymphoblastoid cell lines derived from normal and five MSUD patients, in whom E1 beta was not detected by immunoblot analysis, gave the same restriction maps on Southern blot analysis. The gene has at least 80 kbp.
Abstract 1677: Expression of lysophosphatidic acid acyltransferase-beta in human pancreatic ductal adenocarcinoma
Authors: Michelle A. Blaskovich, Domenico Coppola, James Helm, Said Sebti, Gregory M. Springett
Journal: Cancer Research (2011)

Abstract Lysophosphatidic acid acyltransferase beta (LPAAT-β) is a new therapeutic target and prognostic marker in human cancer. The enzymatic function of LPAAT- β is to convert Lysophosphatidic Acid (LPA) to Phosphatidic Acid (PA), a lipid second messenger that functions to regulate the proliferation, adhesion, migration and survival of cancer cells. PA is an essential cofactor needed by the oncogenes c-Raf, BRAF, PKC-ζ and mTOR for their enzymatic and growth promoting activities in cancer. LPAAT-β protein over expression has been detected in tumors compared to normal tissue in ovarian, endometrial, breast and lung cancers. The over expression of LPAAT-β in ovarian tumors is associated with poor clinical outcome. Methods: In this study we determined LPAAT-β expression in paired specimens of pancreatic ductal adenocarcinoma and adjacent non-malignant pancreas obtained from 102 patients who underwent pancreatectomy with curative intent (stage IA-IIB). Formalin-fixed, paraffin-embedded core sections in a tissue microarray were immunostained using the avidin-biotin-peroxidase method with rabbit anti- LPAAT-β antibody. A semi-quantitative measure of LPAAT-β expression was determined as the product of immunostain intensity and percentage of cells stained, where intensity and percentage were each scored on a scale of 0 to 3, with 3 being maximal. Results: LPAAT-β expression (product score) in 57% (58/102) of pancreatic cancers was lower than in paired non-malignant pancreas, equal in 20% (20/102) of cancers, and greater in 23% (24/102) of cancers as compared to non-malignant pancreas. LPAAT-β expression in cancer (median product score = 3) was less than in non-malignant pancreas (median score = 4) (Wilcoxon signed-rank test, p &amp;lt; 0.0001). Conclusions: Low LPAAT-β expression in pancreatic cancer as compared to non-malignant pancreas stands in contrast to previous studies showing increased LPAAT-β expression in several other cancers. This finding indicates that there may be tissue-specific differences in the biologic activity of LPAAT-β. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1677. doi:10.1158/1538-7445.AM2011-1677
Community involvement in the ethical review of genetic research: lessons from American Indian and Alaska Native populations.
Authors: Richard R Sharp, Morris W Foster
Journal: Environmental Health Perspectives (2002)

The National Bioethics Advisory Commission has proposed that regulatory oversight for research with human subjects be extended beyond the protection of individual research participants to include the protection of social groups. To accomplish this, the commission recommends that investigators and ethics review boards a) work directly with community representatives to develop study methods that minimize potential group harms, b) discuss group implications as part of the informed consent process, and c) consider group harms in reporting research results. We examine the utility of these recommendations in the context of research with American Indian and Alaska Native communities. Because much attention has been given to the question of how best to consult with members of these communities in the design and conduct of research, we believe it behooves investigators to consider the lessons to be learned from research involving American Indians and Alaska Natives. After describing several difficulties surrounding the application of the commission's approach to these research contexts, we propose a research agenda to develop best practices for working with local communities in the ethical assessment of epidemiologic and environmental health research.
A4.16 High Molecular Weight Adiponectin and Tumor Necrosis Factor-A Influence Rheumatoid Adipose-Derived Mesenchymal Stem Cells Function
Authors: U Skalska, W Maslinski, E Kontny
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background and Objectives</h3> Adipose-derived mesenchymal stem cells (ASCs) are promising target in autoimmune diseases therapy. Adipocytokines are known to influence immune system and their role in rheumatoid arthritis (RA) is intensively studied. Objectives of our work were: to investigate whether leptin, low (LMW) and high (HMW) molecular weight adiponectin isoforms and TNF affect IL-6, IL-8, VEGF and TGFβ secretion by RA-ASCs. to determine if conditioned media from RA-ASCs treated with above mentioned adipocytokines, influence function of rheumatoid FLS: IL-6 and MMP-3 secretion, proliferation and apoptosis. <h3>Materials and Methods</h3> Articular adipose tissue and synovial membrane were obtained from 18 RA patients during total knee joint replacement surgery. ASCs were isolated and cultured with/without human recombinant leptin, TNF, adiponectin (LMW, HMW) and IFNγ. After 24 h, secretion of cytokines was measured by ELISA. Conditioned media from ASCs cultures were used to stimulate FLS. After stimulation, FLS proliferation and apoptosis was determined (by incorporation of BrdU and flow cytometry, respectively). Cytokine concentration in FLS culture supernatants was determined by ELISA. The Wilcoxon signed-rank test was used for statistical analysis. <h3>Results</h3> HMW adiponectin enhanced IL-6, IL-8, VEGF (p &lt; 0.001) and TGFβ (p &lt; 0.01) production. LMW adiponectin increased secretion of IL-6 and VEGF (p &lt; 0.001), but its influence was much weaker than HMW9s. TNF was the most potent in stimulating IL-6 and IL-8 production by ASCs (p &lt; 0.001). The influence of adipocytokine treated-ASC-conditioned media on FLS was assesed by comparison with two controls: untreated ASC-conditioned medium and control FLS medium. ASC-conditioned medium treated with HMW caused down-regulation of IL-6 secretion by FLS comparing to both controls (p &lt; 0.05). TNF-treated ASC-conditioned medium induced significant increase in MMP-3 production by FLS comparing to both controls (p &lt; 0.05). Proliferation of FLS was up-regulated after untreated ASC-conditioned medium (p &lt; 0.01) and this was partly reversed by HMW and TNF-treated ASC-conditioned media (p &lt; 0.05). HMW-treated ASC-conditioned medium increased percentage of FLS in late apoptosis (p &lt; 0.05). <h3>Conclusions</h3> HMW and TNF seem to be the most potent in altering RA-ASCs properties. Leptin had no effect and LMW exerted only slight effect. HMW was able to up-regulate factors thought to mediate ASCs immunosupression (TGFβ, IL-6). In addition, its effect on FLS shows that this adipokine may exert positive effect on immunosupressive ASCs function. However, because HMW up-regulated also proangiogenic cytokines (IL-8, VEGF), it is difficult to determine its real impact on RA-ASCs. TNF role in modulating RA-ASCs also need to be elucidated. <h3>Funding</h3> Grant No. N/NZ5/00932, National Science Center, Poland.
Dwarfism with Elevated Levels of Plasma Growth Hormone
Authors: Samir S. Najjar, Avedis K. Khachadurian, Michel N. Ilbawi, Robert M. Blizzard
Journal: New England Journal of Medicine (1971)

Abstract The elevated plasma levels of human growth hormone of two siblings with severe proportional dwarfism did not decrease after glucose administration and rose further on stimulation with argi...
optimization through lateralization: the evolution of handedness
Authors: robert l. sainburg, robert b. eckhardt
Journal: Behavioral and Brain Sciences (2005)

the target article proposes that behavioral asymmetries evolved in response to social pressures, accounting for the unequal distribution of handedness across the population. in contrast, we provide evidence that human handedness reflects individual adaptations that enhance movement skill, and that the distribution across the population is best explained by a genetic polymorphism, either balanced or tending toward fixation for right-handedness.
(27) Porphyria cutanea tarda, systemic lupus erythematosus and vitiligo
Authors: S. Walton, E.H. Wyatt
Journal: British Journal of Dermatology (1987)

Journal Article (27) Porphyria cutanea tarda, systemic lupus erythematosus and vitiligo Get access S. Walton, S. Walton Hull Royal Infirmary Search for other works by this author on: Oxford Academic Google Scholar E.H. Wyatt E.H. Wyatt Hull Royal Infirmary Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 117, Issue s32, 1 June 1987, Pages 75–76, https://doi.org/10.1111/j.1365-2133.1987.tb12067.x Published: 01 June 1987
Idiopathic Unconjugated Hyperbilirubinemia (Gilbert's Syndrome)
Authors: L. W. Powell, Elaine Hemingway, Barbara H. Billing, Sheila Sherlock
Journal: New England Journal of Medicine (1967)

IN 1901 Gilbert and Lereboullet1 described the syndrome of chronic, benign, intermittent jaundice, which was later shown to be distinct from hemolytic disease2 and to be associated with a retention of unconjugated bilirubin in the plasma. It is now known that there are many causes of unconjugated hyperbilirubinemia in addition to hemolytic disease. These include an inherited deficiency of hepatic glucuronyl transferase,3 , 4 shunt hyperbilirubinemia,5 drugs such as rifamycin6 and novobiocin,7 cholecystographic agents8 and portacaval-shunt surgery.9 It is also said to follow viral hepatitis and a wide variety of acquired diseases.4 , 10 , 11 When these conditions have been excluded, there is still the . . .
Tainted Diet Drugs
Authors: Bridget M. Kuehn
Journal: JAMA (2009)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
What is fetal nuchal translucency?
Authors: A. Berger
Journal: BMJ (1999)

In the fetus fluid collects behind the neck, much like it does in dependent ankle oedema in later life. This occurs partly because of the fetus's tendency to lie on its back and partly because of the laxity of the skin of the neck. As with ankle oedema this accumulation of fluid can represent the end point of several pathological processes, including heart failure. Fluid collecting behind the neck can be detected as nuchal translucency by ultrasound scanning, and it can be measured. …
Improvement in myocardial performance without a decrease in high-energy phosphate metabolites after isoproterenol in Syrian cardiomyopathic hamsters.
Authors: S A Camacho, J Wikman-Coffelt, S T Wu, T A Watters, E H Botvinick, R Sievers, T L James, G Jasmin, W W Parmley
Journal: Circulation (1988)

To determine the effect of isoproterenol on cardiac energetics and function in an animal preparation of cardiomyopathy, we studied Langendorff perfused hearts from Syrian cardiomyopathic hamsters. High-energy phosphate metabolites (phosphocreatine [PCr], ATP, inorganic phosphate [Pi]) and intracellular pH (pHi) were measured by 31P nuclear magnetic resonance spectroscopy and correlated with left ventricular developed pressure, coronary flow, and O2 consumption before and during a 10(-6)M infusion of isoproterenol. Total intracellular calcium was also determined by atomic absorption spectroscopy with the use of potassium ethylenediamine tetra-acetate cobaltate as a marker for extracellular space. In cardiomyopathic hamsters, isoproterenol infusion increased mean developed pressure by 300% (p less than .005 compared with control; n = 5), O2 consumption eightfold (p less than .0005), and PCr by 40% (p less than .05). PCr/Pi ratio, which is analogous to phosphorylation potential, improved 100% (p = .05). In normal hamsters, isoproterenol infusion resulted in an 83% increase in developed pressure (p less than .001) and a 25% increase in O2 consumption (NS). However, mean PCr and PCr/Pi decreased by 30% and 50%, respectively (p less than .05 for both), during isoproterenol infusion. pHi decreased in normal animals (p less than .01), but tended to improve in diseased animals (NS) during isoproterenol infusion. Freeze-clamp measurements of phosphate metabolites correlated well with the nuclear magnetic resonance data. Intracellular calcium increased from 0.0102 +/- 0.002 to 0.144 +/- 0.030 mumol/ml heart water in normal hamsters during isoproterenol infusion. Cardiomyopathic hamsters had a markedly elevated baseline calcium content of 60.82 +/- 5.85 mumol/ml heart water due to the presence of dystrophic calcification.(ABSTRACT TRUNCATED AT 250 WORDS)
A Page from My Oncology Fellowship
Authors: Mansoina Baweja
Journal: Annals of Internal Medicine (2005)

On Being a Doctor20 September 2005A Page from My Oncology FellowshipMansoina Baweja, MDMansoina Baweja, MDFrom Mayo Clinic, Jacksonville, FL 32224.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-143-6-200509200-00012 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Lauren was 26 years old when she received the diagnosis of advanced, aggressive lymphoma. She started chemotherapy at my institution when I was a first-year hematology–oncology fellow. She traveled 2 hours to get to the hospital, where she would be admitted for a few days at a time to complete each chemotherapy cycle. Most people took an instant liking to Lauren. Basically cheerful, she remained upbeat despite the difficult chemotherapy, which required intrathecal injections every other week. She had an 8-month-old son, and she was in and out of the hospital in a few days every time. In all those ... Author, Article, and Disclosure InformationAffiliations: From Mayo Clinic, Jacksonville, FL 32224.Corresponding Author: Mansoina Baweja, MD, Mayo Clinic, 4500 San Pablo, Jacksonville, FL 32224; e-mail, Baweja.[email protected]edu. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 20 September 2005Volume 143, Issue 6Page: 463KeywordsBlood countsChemotherapyEmotionsFellowshipsHematology and oncologyLungsLymphomaMemoryResidencyTransplantation ePublished: 20 September 2005 Issue Published: 20 September 2005 Copyright & PermissionsCopyright © 2005 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...