tbuckley/cabot-search huggingface dataset:
Stat3 mediates myeloid cell–dependent tumor angiogenesis in mice
Authors: Maciej Kujawski, Marcin Kortylewski, Heehyoung Lee, Andreas Herrmann, Heidi Kay, Hua Yu
Journal: Journal of Clinical Investigation (2008)

The underlying molecular mechanisms that cause immune cells, mediators of our defense system, to promote tumor invasion and angiogenesis remain incompletely understood. Constitutively activated Stat3 in tumor cells has been shown to promote tumor invasion and angiogenesis. Therefore, we sought to determine whether Stat3 activation in tumor-associated inflammatory cells has a similar function. We found that Stat3 signaling mediates multidirectional crosstalk among tumor cells, myeloid cells in the tumor stroma, and ECs that contributes to tumor angiogenesis in mice. Myeloid-derived suppressor cells and macrophages isolated from mouse tumors displayed activated Stat3 and induced angiogenesis in an in vitro tube formation assay via Stat3 induction of angiogenic factors, including VEGF and bFGF. Stat3-regulated factors produced by both tumor cells and tumor-derived myeloid cells also induced constitutive activation of Stat3 in tumor endothelium, and inhibiting Stat3 in ECs substantially reduced in vitro tumor factor-induced endothelial migration and tube formation. In vivo assays demonstrated the requirement for Stat3 signaling in tumor-associated myeloid cells for tumor angiogenesis. Our results indicate that, by virtue of the ability of Stat3 in tumor cells and tumor-derived myeloid cells to upregulate expression of factors that activate Stat3 in ECs, Stat3 mediates multidirectional crosstalk among tumor cells, tumor-associated myeloid cells, and ECs that contributes to tumor angiogenesis.
A New Luminescence Assay for Autoantibodies to Mammalian Cell–Prepared Insulinoma-Associated Protein 2
Authors: Peter D. Burbelo, Hiroki Hirai, Hannah Leahy, Ake Lernmark, Sten A. Ivarsson, Michael J. Iadarola, Abner Louis Notkins
Journal: Diabetes Care (2008)

OBJECTIVE—Insulinoma-associated protein 2 (IA-2) is a major autoantigen in type 1 diabetes, and IA-2 autoantibodies are routinely detected by a liquid-phase radioimmunoprecipitation assay. The present experiments were initiated to develop a new assay that does not require the use of radioisotopes or autoantigens prepared in bacteria or by in vitro transcription/translation. RESEARCH DESIGN AND METHODS—IA-2 luciferase fusion protein was expressed in mammalian cells and assayed for autoantibodies by liquid-phase luciferase immunoprecipitation. RESULTS—Our study showed that there was no significant difference between the luciferase immunoprecipitation and the radioimmunoprecipitation assays in sensitivity and specificity, and comparison of the two assays revealed a high correlation coefficient (R2 = 0.805). CONCLUSIONS—The luciferase system offers a robust, inexpensive, nonradioactive method for the detection of autoantibodies to mammalian cell–prepared IA-2 and could be of practical value at the clinical level.
How to Use Patient Safety Indicators to Monitor Vascular Surgery Technologies
Authors: Paul N. Suding, Lamont D. Paxton
Journal: JAMA Surgery (2014)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal
Law &amp; medicine. Diabetes. 2
Authors: A. R. Holder
Journal: JAMA (1973)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Changes in Bile Lipids Accompanying Oophorectomy in a Premenopausal Woman
Authors: Lynn J. Bennion
Journal: New England Journal of Medicine (1977)

That ovarian secretions may influence the formation of gallstones is suggested by several observations. In the first place, the excess prevalence of gallstones among women appears shortly after the menarche.1 2 3 Secondly, estrogen-replacement therapy in postmenopausal women and oral-contraceptive treatment in premenopausal women increase the risk of gallbladder disease,4 5 6 7 as does estrogen administration in men.8 Thirdly, oophorectomy in the rat is accompanied by a decrease in the ratio of cholesterol to its solubilizing lipids — bile acids and phospholipids — in bile.9 Fourthly, administration of exogenous estrogens and progestins to animals and human beings raises the ratio of biliary cholesterol to . . .
Practical Parthenote Policy and the Practice of Science
Authors: Lisa Campo-Engelstein, Sarah Rodriguez, Candace Tingen, Teresa Woodruff
Journal: The American Journal of Bioethics (2011)

We wrote our article, “An Obscure Rider Obstructing Science: The Conflation of Parthenotes with Embryos in the Dickey-Wicker Amendment,” out of concern for the practical effects of the Dickey-Wicke...
THE ILL-HEALTH OF RICHARD WAGNER.
Authors: GEORGE M. GOULD
Journal: JAMA (1903)

The following quotations are from the life by Glasenapp and Ellis, and from the volumes of letters to Heckel, Wesedonck and Liszt. I regret exceedingly that the reproduction here of so large a number of excerpts is necessary. I have omitted many that seemed possible to be spared and not spoil the clinical picture. Only by the careful reading can one get a clear idea of Wagner's sufferings and symptoms, and be thus enabled to come to a sound diagnosis of the one malady that resulted in such various and lifelong symptoms. Richard Wagner was born in 1813. His delicate constitution required peculiar care, for he was already troubled with that irritating form of erysipelas (erythema or eczema?) which recurred at intervals throughout life... Down to his sixth year he had no regular lessons; the mother wished to give him time to pick up strength, and would not have plagued
Management of persistent gastric bleeding in a patient with Glanzmann's thrombasthenia
Authors: Suzanne Bakdash, John M. Lyons, Sheldon I. Bastacky, Michael A. Pezzone, James B. McGee, Robert E. Schoen, Miguel Regueiro, Kenneth K. Lee, Franklin A. Bontempo
Journal: American Journal of Hematology (2008)

presentation and evolution of a real clinical case, reacting to clinical information and data (boldface type).
984 Molecular profiles of drug response in HCV infected patients during the first 4 weeks of therapy for chronic hepatitis C virus with pegylated interferon containing regimens or albuferon™
Authors: V BALAN, D NELSON, M SULKOWSKI, M ROSATI, C BIRSE, J PRAESTGAARD, B OSBORN, H MESGHALI, L NOVELLO, O PICKERAL
Journal: Hepatology (2003)

Interferons (IFNs) exert their anti-viral effects by inducing the expression of hundreds of IFN-stimulated genes (ISGs). The activity of known ISGs is insufficient to account for the antiretroviral effects of IFN, suggesting that ISGs with antiretroviral activity are yet to be described. We constructed an arrayed library of ISGs from rhesus macaques and tested the ability of hundreds of individual macaque and human ISGs to inhibit early and late replication steps for 11 members of the retroviridae from various host species. These screens uncovered numerous ISGs with antiretroviral activity at both the early and late stages of virus replication. Detailed analyses of two antiretroviral ISGs indicate that indoleamine 2,3-dioxygenase 1 (IDO1) can inhibit retroviral replication by metabolite depletion while tripartite motif-56 (TRIM56) accentuates ISG induction by IFNα and inhibits the expression of late HIV-1 genes. Overall, these studies reveal numerous host proteins that mediate the antiretroviral activity of IFNs.
Repair of aortico-left ventricular tunnel in the neonate: surgical, anatomic and echocardiographic considerations.
Authors: K Turley, N H Silverman, D Teitel, C Mavroudis, R Snider, A Rudolph
Journal: Circulation (1982)

Repair of aortico--left ventricular tunnel was accomplished for the first time in the neonatal period. The diagnosis was made with two-dimensional echocardiography. Repair was accomplished using an open-patch aortoplasty technique, which prevented valvular insufficiency, a significant problem in older patients in whom the repair has been attempted.
The danger of denial
Authors: Des Spence
Journal: BMJ (2004)

Have you ever caught a glimpse of yourself in a mirror and thought, “Oh, that's just a bad angle.” How many of us men have seen the top of our head in the monitor of a security camera and said to ourselves, “It's just a very bright light.” In psychobabble this is known as denial, one of the most popular of the “coping strategies.” I like using denial as a strategy, as it is easy, convenient, effective, and applicable to almost every life situation. My vanity about my baldness and weight is a source of amusement to my friends and family and is harmless. However, what happens when our denial affects other people, even lots of other people, such as our patients?

I used denial in my relationship with the pharmaceutical industry and how this relationship affected my practice and patients. I wanted to believe that all the “freebies” and “fees” were justifiable and that my frequent contact with the industry did not affect my practice. Yes, I tended …
Heparin and Thrombocytopenia
Authors: MANUEL RAMIREZ-LASSEPAS
Journal: Annals of Internal Medicine (1984)

Letters and Corrections1 April 1984Heparin and ThrombocytopeniaMANUEL RAMIREZ-LASSEPAS, M.D., ROBERT J. CIPOLLE, PHARM, D.MANUEL RAMIREZ-LASSEPAS, M.D., ROBERT J. CIPOLLE, PHARM, D.Author, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-100-4-613_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: In his report on heparin-associated thrombosis without thrombocytopenia Phelan (1) reports the case of a patient who had three ischemic events associated with large drops in platelet counts (59%, 46%, and 93% of pretreatment counts) that occurred during continuous intravenous heparin therapy. In the first two instances, even though platelet counts dropped by more than 100 000/mm3, counts did not reach a "thrombocytopenic" level (110 000 and 187 000).We have recently called attention to this phenomenon in patients treated with continuous intravenous heparin for ischemic cerebrovascular disease (2). We saw an association between poor outcome (ischemic...References1. PHELAN B. Heparin-associated thrombosis without thrombocytopenia. Ann Intern Med. 1983;99:637-8. LinkGoogle Scholar2. RAMIREZ-LASSEPASCIPOLLERODVOLD MRK. Heparin-induced thrombocytopenia in patients with cerebrovascular ischemic disease. Neurology. 1984. (In press). CrossrefGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: St. Paul-Ramsey Medical Center St. Paul, MN 55101 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByHeparin-induced thrombocytopenia.Heparin-Induced Thrombocytopenia and Thrombosis in Ischemic Stroke 1 April 1984Volume 100, Issue 4Page: 613-613KeywordsCerebrovascular diseasesHeparinPlateletsThrombosis Issue Published: 1 April 1984 PDF DownloadLoading ...
18F-FDG and 11C-Methionine PET/CT Findings in a Case With Anti-NMDA (NR2B) Receptor Encephalitis
Authors: Miyako Morooka, Kazuo Kubota, Ryogo Minamimoto, Mariko Furuhata, Tadashi Abe, Kimiteru Ito, Momoko Okasaki, Kenji Ishii, Kiichi Ishiwata
Journal: Clinical Nuclear Medicine (2012)

A 70-year-old man was brought to the emergency room in an unconscious state. A cerebrospinal fluid analysis demonstrated strongly positive autoantibody glutamate receptor epsilon 2 (GluRε2) titer, corresponding to anti-N-methyl-D-aspartate receptor (anti-NMDAR) NR2B autoantibody titers. The patient was diagnosed with limbic encephalitis. Brain MRI showed a high intensity in the left anterior-temporal lobe on T2WI/fluid attenuated inversion recovery (FLAIR) images, without enhancement. In this area, 18F-FDG PET showed a relatively decreased uptake, but 11C-methionine PET showed mildly increased uptake. Despite the patient's near-full functional recovery, similar PET findings were confirmed 6 months later. The mechanism of regional cerebral increased 11C-methionine uptake in anti-NMDAR (NR2B) receptor encephalitis is unknown.
James Wilson Harkess
Authors: Donald A L Dick
Journal: BMJ (2007)

Born June 1925 in Edinburgh, James Wilson Harkess attended George Heriot School and then Edinburgh University, graduating in 1948.

A lifelong scholar, he was most grateful for his education and to his teachers. In turn, he inspired his students and graduates with his enthusiasm, conscientiousness, attention to detail, and a desire to know how and why things worked in science and medicine.

Completing house appointments, James was drafted for UK military service, serving mostly in Malaya and Singapore. He returned to Princess Margaret Rose Hospital to continue his interest in orthopaedics, and from there he sought further experience in the United States at the Albany Hospital, Albany, New York. As an outstanding resident, he was asked to stay on and complete its surgical programme.

Ironically, …
Traumatic Fibrosis of the Rectus Femoris Muscle
Authors: Michael R. Rask
Journal: JAMA (1972)

Although injury to the rectus femoris is a very common occurrence, resultant fibrosis of the muscle is rare. The pathogenesis of the injury is in effect a third-degree tear of the muscle with retraction of the muscle and hemorrhage with a resultant fibrotic mass. Of five patients with traumatic fibrosis of the rectus femoris muscle, three patients had surgical extirpation of the fibrotic mass with good return of quadriceps function, and one had acute partial rupture of the rectus femoris muscle with spontaneous recovery.
Breast feeding does not always work
Authors: Jenny Bennison
Journal: BMJ (1997)

I was 38 weeks pregnant and had finished my last surgery earlier the previous day. At 4 am I was admitted to hospital and less than an hour later Sapphire had arrived. Her Apgar scores were 9 and we had a memorable hour on our own with this alert and happy little person. Her birth weight was 2450 g but her blood sugars were fine, and I made sure that she received only my colostrum.

Sapphire came home after 48 hours. By now she was beautiful, although beginning to turn yellow, and at home she seemed particularly small …
Tight Control of Exogenous SERCA Expression Is Required to Obtain Acceleration of Calcium Transients With Minimal Cytotoxic Effects in Cardiac Myocytes
Authors: J. Michael O’Donnell, Carlota M. Sumbilla, Hailun Ma, Iain K. G. Farrance, Marco Cavagna, Michael G. Klein, Giuseppe Inesi
Journal: Circulation Research (2001)

Abstract —Collateral effects of exogenous sarcoendoplasmic reticulum Ca 2+ ATPase (SERCA) expression were characterized in neonatal rat and chicken embryo cardiac myocytes, and the conditions required to produce acceleration of Ca 2+ transients with minimal toxicity were established. Cultured myocytes were infected with adenovirus vector carrying the cDNA of wild-type SERCA1, an inactive SERCA1 mutant, or enhanced green fluorescence protein under control of the cytomegalovirus promoter. Controls were exposed to empty virus vector. Each group was tested with and without phenylephrine (PHE) treatment. Under conditions of limited calf-serum exposure, the infected rat myocytes manifested a more rapid increase in size, protein content, and rate of protein synthesis relative to noninfected controls. These changes were not accompanied by reversal to fetal transcriptional pattern (as observed in hypertrophy triggered by PHE) and may be attributable to facilitated exchange with serum factors. SERCA virus titers &gt;5 to 6 plaque-forming units per cell produced overcrowding of ATPase molecules on intracellular membranes, followed by apoptotic death of a significant number of rat but not chicken myocytes. Enhanced green fluorescence protein virus and empty virus also produced cytotoxic effects but at higher titers than SERCA. Expression of exogenous SERCA and enhancement of Ca 2+ transient kinetics could be obtained with minimal cell damage in rat myocytes if the SERCA virus titer were maintained within 1 to 4 plaque-forming units per cell. Expression of endogenous SERCA was unchanged, but expression of exogenous SERCA was higher in myocytes rendered hypertrophic by treatment with PHE than in nontreated controls.
A guide to the radiographic identification of prosthetic heart valves.
Authors: D J Mehlman, L Resnekov
Journal: Circulation (1978)

To facilitate the radiographic identification of prosthetic heart valves, radiographic silhouettes of various aortic, mitral and tricuspid valve prostheses were analyzed and shematized. Forty different prosthetic valve models (heterograft and mechanical) were obtained from seven domestic manufacturers. Each prosthesis was positioned to approximate the aortic, mitral or tricuspid valve anulus plane. The valves were then radiographed and photographed as they would appear in posteronanterior and left lateral chest radiographs. The radiographic silhouettes were examined and a scheme was designed to enable easy, yet accurate, differentiation of the many heart valve prostheses. Illustrations of the various valve types are presented as they would appear in routine chest radiographs.
Liposuction does not achieve metabolic benefits of weight loss
Authors: Scott Gottlieb
Journal: BMJ (2004)

Abdominal liposuction does not significantly improve metabolic abnormalities associated with obesity, and removing adipose tissue mass alone does not achieve the metabolic benefits of weight loss, a new research report says ( New England Journal of Medicine 2004;350:2549-57).

Abdominal obesity is associated with insulin resistance and other metabolic risk factors for coronary heart disease. Although both the abdominal subcutaneous fat mass and the visceral fat mass are associated with insulin resistance, it is not known whether one or both of these fat depots are actually involved in the pathogenesis of insulin resistance or whether they are simply associated with the metabolic complications of obesity. …
Human megakaryocytes. V. Changes in the phenotypic profile of differentiating megakaryocytes.
Authors: R B Levene, J M Lamaziere, H E Broxmeyer, L Lu, E M Rabellino
Journal: The Journal of Experimental Medicine (1985)

Human megakaryocytes were studied for phenotypic changes occurring throughout differentiation using a panel of monoclonal antibodies raised against marrow megakaryocytes and blood platelets. 11 monoclonal antibody preparations were selected for restricted specificity against megakaryocytes and/or platelets after screening by immunofluorescence, complement-mediated cytolysis, and solid phase enzyme-linked immunosorbent assay. The expression of the cellular epitopes recognized by these reagents enabled the identification of three levels of megakaryocyte maturation characterized by distinct immunologic phenotypes. Based upon their reactivities against megakaryocytic cells at different ontogenetic levels, monoclonal antibodies were operationally categorized into three groups. Group A consisted of six different monoclonal antibodies that recognized antigens on the colony-forming unit-megakaryocyte (CFU-Mk), in vitro grown colony megakaryocytes, and early immature marrow megakaryocytes, only, and did not detect their respective epitopes on either mature megakaryocytes or platelets. A monoclonal antibody categorized in group B detected a cell antigen expressed by megakaryocytic cells at all maturational levels, but which is lost or suppressed during terminal differentiation and is not expressed on blood platelets. Group C included four different monoclonal antibodies raised against platelets that recognized antigenic determinants expressed on the CFU-Mk, colony megakaryocytes, early and mature megakaryocytes, and platelets. Three group C monoclonal antibodies (PC-1, PC-3, and PC-4) were specific for platelet glycoprotein IIb/IIIa. Additionally, group C monoclonal antibody PC-2 was unique in that it showed partial reactivity against the clonable progenitor for the erythroid series (BFU-E). Recognition of discrete phenotypic changes in differentiating megakaryocytes will enable multiparameter analyses of these cells as well as the study of factors regulating the dynamics of megakaryocytopoiesis in health and disease.
Junctional Adhesion Molecule-A Is Required for Hematogenous Dissemination of Reovirus
Authors: Annukka A.R. Antar, Jennifer L. Konopka, Jacquelyn A. Campbell, Rachel A. Henry, Ana L. Perdigoto, Bruce D. Carter, Ambra Pozzi, Ty W. Abel, Terence S. Dermody
Journal: Cell Host & Microbe (2009)
Roles of Diversifying Selection and Coordinated Evolution in the Evolution of Amphibian Antimicrobial Peptides
Authors: Thomas F. Duda, Damien Vanhoye, Pierre Nicolas
Journal: Molecular Biology and Evolution (2002)

Antimicrobial peptides are expressed in the skin of amphibians and are used to prevent infection by microorganisms. Frog species store distinct collections of antimicrobial peptides that show variation in size, charge, conformation, and bactericidal activity, and so the evolution of antimicrobial peptide gene families may reflect the adaptive diversification of these loci. We examined the molecular evolution of antimicrobial peptide transcripts from hylid and ranid frog species. Our results show that after the gene family arose in the common ancestor of the Hylidae and Ranidae, before the divergence of these families in the Mesozoic, it subsequently diversified within these groups with numerous duplication events and divergence of loci. Moreover, we provide evidence that suggests that members of the antimicrobial peptide gene family have been subject to diversifying selection within both propiece and mature domains of hylids and solely within the mature domain of ranids. Finally, our results suggest that coordinated and compensatory amino acid replacements have occurred within the acidic propiece and cationic mature domain of hylid antimicrobial peptide precursors, as has been observed for mammalian defensin genes, but not among those of ranid precursors.