Sensory innervation around immediately vs. delayed loaded implants: a pilot study
Authors: Yan Huang, Jeroen van Dessel, Wendy Martens, Ivo Lambrichts, Wei-Jian Zhong, Guo-Wu Ma, Dan Lin, Xin Liang, Reinhilde Jacobs
Journal: International Journal of Oral Science (2014)

Although neurophysiological and psychophysical proof of osseoperception is accumulating, histomorphometric evidence for the neural mechanisms of functional compensation following immediate and delayed implant loading is still lacking. For this randomized split-mouth study, six mongrel dogs randomly received one of four treatment protocols at 36 implant-recipient sites over 16 weeks (third maxillary incisor, third and fourth mandibular premolar): immediate implant placement and immediate loading (IIP+IL); delayed implant placement and delayed loading (DIP+DL); delayed implant placement and immediate loading (DIP+IL); and natural extraction socket healing (control). Histomorphometry was performed in the peri-implant bone and soft tissues within 300 µm around the implants. Immunocytochemistry and transmission electron microscopy were used to confirm the presence of neural structures and to reveal their ultrastructural characteristics, respectively. Myelinated nerve fibres densely populated the peri-implant crestal gingival and apical regions, although they were also identified in the woven bone and in the osteons near the implant threads. Compared with the control group in the mandible, the group that received IIP+IL showed a higher innervation (in N⋅mm−2, 5.94±1.12 vs. 3.15±0.63, P<0.001) and smaller fibre diameter (in µm, 1.37±0.05 vs. 1.64±0.13, P=0.016), smaller axon diameter (in µm, 0.89±0.05 vs. 1.24±0.10, P=0.009) and g-ratio (0.64±0.04 vs. 0.76±0.05, P<0.001) in the middle region around the implants. Compared with DIP+IL in the mandible, IIP+IL had a higher nerve density (in N⋅mm−2, 13.23±2.54 vs. 9.64±1.86, P=0.027), greater fibre diameter (in µm, 1.32±0.02 vs. 1.20±0.04, P=0.021), greater axon diameter (in µm, 0.92±0.01 vs. 0.89±0.03, P=0.035) and lower g-ratio (0.69±0.01 vs. 0.74±0.01, P=0.033) in the apical region around the implants. It may be assumed that the treatment protocol with IIP+IL is the preferred method to allow optimized peri-implant re-innervation, but further functional measurements are still required. Researchers in China and Belgium show that immediate implant placement and loading favours sensory nerve regrowth in dogs. Tooth extraction can damage nerve endings that signal information about tooth loads and which are essential for chewing but little is known about the influence of implant loading on their recovery. A randomized study led by Xin Liang at Dalian Medical University, China, examined the effects of immediate and delayed implant placement and loading on sensory reinnervation in six dogs. They found that compared with control (natural socket healing after extraction) and delayed implant placement (eight weeks after tooth extraction), immediate implant placement and loading led to the highest nerve density and largest nerve fiber diameters 16 weeks after surgery. These findings highlight the importance of timely implant placing and loading in optimizing nerve recovery.