Amyloid in Urinary Sediments as a Diagnostic Technique
Authors: JOSHUA SHEMER
Journal: Annals of Internal Medicine (1979)

Brief Reports1 January 1979Amyloid in Urinary Sediments as a Diagnostic TechniqueJOSHUA SHEMER, M.D., GLENDA YAEL MESSER, Ph.D., MORDECHAI PRAS, M.D., JOSEPH GAFNI, M.D.JOSHUA SHEMER, M.D.Search for more papers by this author, GLENDA YAEL MESSER, Ph.D.Search for more papers by this author, MORDECHAI PRAS, M.D.Search for more papers by this author, JOSEPH GAFNI, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-90-1-61 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThree separate reports describing the detection of amyloid fibrils in urinary sediment, each in a single amyloidotic patient, suggested a noninvasive technique for the diagnosis of amyloidosis (1-3). Others, however, have reported amyloid fibrils in nonamyloidotic nephrotic patients and normal persons as well (4).To evaluate the diagnostic usefulness of the occurrence of amyloid in the urine, we screened the urines of 20 proteinuric amyloidotic patients and, as control subjects, 10 nonamyloidotic nephrotic patients.Five hundred-millilitre morning urine specimens were centrifuged in a Sorval superspeed RC-2-B refrigerated centrifuge (Sorval, Newtown, Connecticut) at 15 000gfor 1 h. The sediment...References1. DEROSENAKOSSPIRANI RMC: Demonstration of amyloid fibrils in the urinary sediment. N Engl J Med 293:1131-1133, 1975 CrossrefMedlineGoogle Scholar2. NEALE T: Amyloid fibrils in urinary sediment (letter). N Engl J Med 294:444, 1975 Google Scholar3. NIMOITYNLASKERSORIANO PMR: Detection of urinary amyloid in Familial Mediterranean fever. Br Med J 2:284-285, 1976 CrossrefMedlineGoogle Scholar4. SHIRAHAMASKINNERCOHENBENSON TMAM: Uncertain value of urinary sediments in the diagnosis of amyloidosis. N Engl J Med 297:821-823, 1977 CrossrefMedlineGoogle Scholar5. WEBEROSBORN KV: The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem 244:4406-4412, 1969 CrossrefMedlineGoogle Scholar6. PRASRESHEF MT: The acid-soluble fraction of amyloid—a fibril forming protein. Biochim Biophys Acta 27:193-203, 1972 CrossrefGoogle Scholar7. FRANKLINPRAS EM: Immunologic studies of water-soluble human amyloid fibrils. J Exp Med 130:797-808, 1969 CrossrefMedlineGoogle Scholar8. LEVINPRASFRANKLIN MME: Immunologic studies of the major nonimmunoglobulin protein of amyloid. I. Identification and partial characterization of a related serum component. J Exp Med 138:373-380, 1973 CrossrefMedlineGoogle Scholar9. PRASGAFNI MJ: The nature of amyloid, in Immunochemistry, edited by GLYNN LE, STEWARD MW, John Wiley and Sons, Chichester, 1977, pp. 509-533 Google Scholar10. LINKE R: Urinary amyloid fibrils in the absence of amyloidosis (letter). Br Med J 2:1259, 1976 CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Chaim Sheba Medical Center at Tel-Hashomer Tel-Aviv University School of Medicine Tel-Aviv Israel PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByAmyloidosisGlomerular DiseaseLa néphropathie amyloïde Etude clinique de 135 observationsMorphologic and clinical correlates in renal amyloidosis 1 January 1979Volume 90, Issue 1Page: 61-62KeywordsAmyloid proteinsAmyloidosisDiagnostic techniquesUrine Issue Published: 1 January 1979 PDF DownloadLoading ...