Globulin Synthesis by Human Peripheral Lymphocytes
Authors: I. J. FORBES
Journal: Annals of Internal Medicine (1966)

Article1 July 1966Globulin Synthesis by Human Peripheral LymphocytesIn Vitro Measurements Using Lymphocytes from Normals and Patients with DiseaseI. J. FORBES, M.D., D. W. HENDERSON, M.B.I. J. FORBES, M.D.Search for more papers by this author, D. W. HENDERSON, M.B.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-65-1-69 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptRecent studies have provided evidence that circulating lymphocytes may synthesize globulin in vitro (1-3). Furthermore, there is good evidence that at least some of this globulin may be antibody (4-6). Globulin synthesis may be stimulated by phytohemagglutinin (2-3).Protein synthesis by human lymphocytes may be quantitated in vitro by measuring, under standard conditions, the incorporation of14C-labeled amino acids into protein (3). These studies have indicated that the capacity of lymphocytes to synthesize globulin in vitro may differ considerably in certain disease states from that observed in normal subjects. Observations are reported in this paper on the function of leukocytes...References1. BACHHIRSCHHORN FK: γ-globulin production by human lymphocytes in vitro. Exp. Cell Res. 32: 592, 1963. CrossrefMedlineGoogle Scholar2. HIRSCHHORNBACHKOLODNYFIRSCHEINHASHEM KFRLILN: Immune response and mitosis of human peripheral blood lymphocytes in vitro. Science 142: 1185, 1963. CrossrefMedlineGoogle Scholar3. FORBESTURNER IJKJ: Synthesis of protein by human lymphocytes in vitro. I. Clinical Studies. Aust. Ann. Med. 14: 304, 1965. MedlineGoogle Scholar4. HULLIGERSORRIN LE: Synthesis of antibodies by blood leucocytes in the rabbit. Nature (London) 198: 299, 1963. CrossrefMedlineGoogle Scholar5. LANDYSANDERSONBERNSTEINJACKSON MRPMTAL: Antibody production by leucocytes in peripheral blood. Nature (London) 204: 1320, 1964. 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VAN FURTH R: The Formation of Immuno-globulins by Human Tissues in Vitro (doctoral thesis). University of Leiden, 1964. Google Scholar14. WILSONKIRKPATRICKTALMAGE WECHDW: Suppression of immunologic responsiveness in uremia. Ann. Intern. Med. 62: 1, 1965. LinkGoogle Scholar15. AISENBERG AC: Studies of lymphocyte transfer reactions in Hodgkin's disease. J. Clin. Invest. 44: 555, 1965. CrossrefMedlineGoogle Scholar16. SOLOMONWALDMANNFAHEY ATAJL: Metabolism of normal 6-6S γ-globulin in normal subjects and in patients with macroglobulinaemia and multiple myeloma. J. Lab. Clin. Med. 62: 1, 1963. MedlineGoogle Scholar17. FORBESMILAZZO IJSC: A metabolic defect of the lymphocytes in a case of hypogammaglobulinaemia. Aust. Ann. Med. 14: 311, 1965. MedlineGoogle Scholar18. BERNARDGERALDESBOIRON CAM: [Effects of phytohaemagglutinin on blood-cultures of chronic lymphocytic leukaemias (letter).] Lancet 1: 667, 1964. CrossrefMedlineGoogle Scholar19. WINTEROSMONDYOFFEYMAHY GCDGJMDJ: Leucocyte cultures with phytohaemagglutinin in chronic lymphatic leukaemia. Lancet 2: 563, 1964. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: I. J. FORBES, M.D.; D. W. HENDERSON, M.B.Affiliations: Woodville, South AustraliaFrom the Department of Medicine, University of Adelaide, The Queen Elizabeth Hospital, Woodville, South Australia.Dr. Forbes is the recipient of a grant from the National Health and Medical Research Council of Australia.Requests for reprints should be addressed to I. J. Forbes, M. D., Department of Medicine, University of Adelaide, The Queen Elizabeth Hospital, Woodville, South Australia. 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Stimulation of hyaluronate synthesis by live and dead leukocytesDie Lymphocytenkultur und ihre Beziehung zu klinischen ProblemenCase 23-1968Synthesis of some Serum Proteins in Lymphocytes stimulated by PhytohaemagglutininEFFECTS OF ANTI-INFLAMMATORY DRUGS ON LYMPHOCYTES 1 July 1966Volume 65, Issue 1Page: 69-79KeywordsAmino acidsAplastic anemiaBlood plasmaDrug therapyGlobulinsHospital medicineLymphocytesPhytohemagglutininsProtein synthesisProteins ePublished: 1 December 2008 Issue Published: 1 July 1966 PDF downloadLoading ...