tbuckley/cabot-search huggingface dataset:
19. rAAV-2 Transduction of Primitive Human Hematopoietic Stem Cells Capable of Serial Engraftment in Immune Deficient Mice
Authors: Helicia Paz, Leah Santat, Lijing Li, Christie Wong, Stephen J. Forman, K.K. Wong
Journal: Molecular Therapy (2005)

An rAAV-2 vector encoding antisense RNA targeting sequences in the HIV-1 LTR including the TAR and polyadenylation sequences common to all HIV transcripts was tested for gene transfer to human cord blood CD34+CD3//Q hematopoietic stem/progenitor cells (HSC) following serial engraftment in immune-deficient NOD/SCID mice. Flow cytometrically purified cord blood stem cells were transduced with rAAV overnight at low cytokine concentrations and transplanted into sub-lethally irradiated recipients. Long-term multi-lineage marking of myeloid, lymphoid and CD34 progenitor cells was readily detected in primary and secondary recipients following transplantation with purified human HSC. Notably, the frequency of rAAV marking was found to be higher in secondary recipients, indicating efficient transduction of primitive HSC. Parallel serial analyses performed in vitro with clonogenic progenitor cells derived from the marrow of transplant recipients confirmed stable and efficient rAAV marking of HSC. Transgene function was investigated in the macrophage progeny of transduced CD34 stem/progenitor cells. Antisense and control transduced CD34 cells were cultured with cytokines to induce macrophage differentiation but were not subjected to positive selection. Three weeks later adherent macrophages were challenged with HIV-1 JR-FL, a monocytotropic strain of HIV, at a multiplicity of 0.01. Long-term inhibition of HIV-1 replication was noted in every donor tested relative to either untransduced or control rAAV transduced cultures, indicating stable and functional transgene expression in the progeny of transduced stem/progenitor cells. Previous reports of rAAV transduction of hematopoietic stem cells in short term in vitro studies have shown conflicting results, possibly due to the recently defined limitations to initiation of transgene expression, including nuclear entry, uncoating and second strand synthesis. However, demonstration of long term rAAV transduction of human stem cells capable of serial engraftment and transgene expression in differentiated progeny supports the use of these vectors for genetic modification of stem cells.
Abstract 548: CEP 3/CEP 17 in the genetic diagnosis and its prognostic relevance of Kazakh esophageal cancer patients
Authors: Edris Awut
Journal: Cancer Research (2014)

Abstract To investigate the clinical application value of centromere of chromosome 3 and 17 (CEP 3, CEP 17) DNA probes in Kazakh esophageal squamous cell carcinoma (ESCC) patients’ molecular pathological diagnosis and prognosis. Forty patients with ESCC who received radical surgical treatment and 10 cases of normal specimen in control group were enrolled in this study. Touch preparations of fresh cancer and normal tissue were made and fluorescence in situ hybridization (FISH) technique were used to count the copy numbers of CEP 3 and CEP 17, abnormalities were analyzed comparing with routine pathological diagnosis. FISH analysis revealed that abnormal copy numbers of CEP 3, CEP 17 (aneuplody) were observed in 40 cases. CEP 3 and CEP 17 polyploidy rates were 42%, 40.5% in well-differentiated, 62.9%, 46.8% in moderately differentiated, and 76.95%, 63.5% in poorly differentiated ESCC respectively. These were significantly different between poorly differentiated, moderately differentiated and well-differentiated groups (p&amp;lt;0.05, p&amp;lt;0.05), and the polyploidy rates were also significantly higher in the group with lymph node metastasis comparing to the group without lymph node metas-tasis (CEP 3, p=0.0001; CEP 17, p=0.012). The CEP 3, 17 variations were shown to be correlated with the level of differentiation and lymph node metastasis in ESCC. Therefore, there DNA probes may be the predictive biological marker in the prognosis of patients with ESCC. In addition, being an objective and qualitative method, the technology of FISH can detect CEP 3, 17 variations in the genetic diagnosis of Kazakh ESCC. Note: This abstract was not presented at the meeting. Citation Format: Edris Awut. CEP 3/CEP 17 in the genetic diagnosis and its prognostic relevance of Kazakh esophageal cancer patients. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 548. doi:10.1158/1538-7445.AM2014-548
Intermittent torsion of the testis.
Authors: T. A. Creagh, T. E. McDermott, P. A. McLean, A. Walsh
Journal: BMJ (1988)

Relative n'sks of disorders related to cotntact lenisesjor caclh tvpe of letns No of No of Relative 95"O Confidence Significance Lens type cases controls risk interval of trend Mecabolic disorders
Young Athletes With Low Back Pain: Skeletal Scintigraphy of Conditions Other Than Pars Interarticularis Stress
Authors: Leonard P. Connolly, Laura A. Drubach, Susan A. Connolly, S Ted Treves
Journal: Clinical Nuclear Medicine (2004)

Skeletal scintigraphy is an important method for showing evidence of stress injuries affecting the partes interarticulares of young athletes with low back pain. Other etiologies of low back pain may also cause uptake abnormalities in these patients. How often do the results of skeletal scintigraphy support diagnoses other than stress injuries to the partes interarticulares and what are these diagnoses?We retrospectively reviewed the records of 209 young patients (149 females, 60 males; age range: 8-21 years, mean: 15.7 years) with low back pain and no previously treated vertebral condition who were consecutively referred from a sports medicine clinic to skeletal scintigraphy.Sites of high uptake supportive of diagnoses other than pars interarticularis stress were shown in 36 (17%) of the 209 patients. Other diagnoses supported by skeletal scintigraphy included stress at the articulation between a transitional vertebra and the sacrum, injuries to the vertebral body ring apophysis, sacral fracture, spinous process injury, and sacroiliac joint stress.Skeletal scintigraphy shows uptake abnormalities supportive of diagnoses other than pars interarticularis stress in a significant number of young patients with low back pain. The uptake abnormalities shown are usually stress-related in this select population.
Correlation of inhaled nitric-oxide induced reduction of pulmonary artery pressure and vascular changes
Authors: B.H. Jiang, J. Maruyama, A. Yokochi, H. Amano, Y. Mitani, K. Maruyama
Journal: European Respiratory Journal (2002)

The purpose of the present study was to determine the relationship between hypertensive pulmonary vascular remodelling and the changes in mean pulmonary artery pressure (mPAP) during low-dose nitric oxide (NO) inhalation. Rats were exposed to chronic hypobaric hypoxia (air at 50.5 kPa (380 mmHg), 10% oxygen, for 5–29 days) to induce chronic pulmonary hypertension (PH) with pulmonary vascular structural changes. After the chronic hypoxic exposure, the rats had an indwelling pulmonary artery catheter inserted and changes in mPAP with NO were correlated to morphometrical analysis of pulmonary vascular changes. All concentrations of inhaled NO (0.1–2.0 parts per million) reduced mPAP with a similar per cent reduction from baseline mPAP in PH rats, while no changes were observed in control rats. During NO inhalation in PH rats, the absolute value of the decrease in mPAP, but not per cent reduction in mPAP, significantly correlated with baseline mPAP, the percentage of muscularised arteries at the alveolar wall level and at the alveolar duct level, and the per cent medial wall thickness of muscularised arteries. In the chronic hypoxic pulmonary hypertension model, the severity of pulmonary vascular remodelling did not alter the reactivity of the pulmonary arteries to nitric oxide and might, in part, determine the magnitude of nitric-oxide induced absolute reduction in mean pulmonary artery pressure.
Depressive Symptoms in Cancer Patients Undergoing Chemotherapy: A Psychometric Analysis
Authors: Thomas Middelboe, Lars Ovesen, Erik Lykke Mortensen, Per Bech
Journal: Psychotherapy and Psychosomatics (1994)

Depressive psychopathology was measured in a prospective follow-up study of 36 cancer patients. The psychometric properties of the Hamilton rating scales for depression and anxiety and the Melancholia Scale were examined. The scales proved useful, indicating that the symptomatic structure of depression in cancer patients is rather identical to that seen in primary depression. Depressive states were found in about 40% of patients before the start of chemotherapy. The frequency of intermediate and high scores on the depression scales showed a low to moderate decrease after 6 months. Aspects of depression in the medically ill are discussed, and screening routines suggested.
Improving the Accuracy of Abstracts in Scientific Articles
Authors: Heather Lodge
Journal: JAMA (1998)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Autoantigen, innate immunity, and T cells cooperate to break B cell tolerance during bacterial infection
Authors: Pauline Soulas, Anne Woods, Benoit Jaulhac, Anne-Marie Knapp, Jean-Louis Pasquali, Thierry Martin, Anne-Sophie Korganow
Journal: Journal of Clinical Investigation (2005)

Autoantibody production during infections is considered to result from nonspecific activation of low-affinity autoreactive B cells. Whether this can lead to autoimmune disease remains uncertain. We show that chronic infection by Borrelia burgdorferi of Tg animals expressing human rheumatoid factor (RF) B cells (of low or intermediate affinities) in the absence or in the constitutive presence of the autoantigen (represented here by chimeric IgG with human constant region) breaks their state of immunological ignorance, leading to the production of RFs. Surprisingly, this production was more pronounced in intermediate-affinity RF Tg mice coexpressing the autoantigen. This overproduction was mediated by immune complexes and involved synergistic signaling between the B cell receptor and Toll-like receptors and T cell help. These findings indicate that chronic infection can activate autoreactive B cells with significant affinity and creates conditions that can drive them to differentiate into memory cells. Such cells may have some physiological yet undetermined role, but in autoimmune-prone individuals, this scenario may initiate autoimmunity.
Plastic Draping Materials
Authors: C. H. Frazier
Journal: JAMA (1971)

To the Editor.—  We recently had a problem with the use of transparent plastic drapes, said to be impermeable to moisture, which I think should be generally known. The patient was a 71-year-old lady with some skin changes on her leg resulting from venous stasis. The drape was in contact with the skin. When the drape was removed, a full-thickness portion of skin also was removed. She had a very good result from a split-thickness skin graft. I simply want to call attention to the possibilities of this problem with the use of this or other similar plastic draping materials.
Life With Dignity
Authors: Robert Raskind
Journal: JAMA (1974)

<h3>To the Editor.—</h3> We would like to add our support to Dr. Levine's concept of "The Chance to Live with Dignity" (226:672,1973). Our efforts to promote this idea have been somewhat less than successful. It is our belief that the "die with dignity" idea has become popular because it is easier to "go along" with a diagnosis already in the record than to insist on rigid criteria for its documentation or to make a new investigation. Less work is involved in allowing a patient to die in the middle of the night than in getting oneself out of bed to start an infusion or some other such "heroic measure." In the health maintenance organization (HMO) and prepaid plans of medical practice, economic saving results from letting the individual go to his reward without "expensive procedures" and an accumulation of days in the hospital. It is our plea to medical practitioners
Two decades of change: Glenside Hospital population surveys 1960-80.
Authors: D F Early, M Nicholas
Journal: BMJ (1981)

Results of the fifth quinquennial survey of the patients of Glenside Hospital, Bristol, when compared with five yearly surveys over the past 20 years, show that the "run-down" of the hospital continues. There is still an accumulation of long-stay patients, mainly for non-medical reasons. They remain in hospital because of the failure to provide community facilities adequate to their needs. It is suggested that local authorities will never be able to cope with them and that the NHS should be enabled to extend their hospital services into "nursing homes" in the community.
THE CONTRIBUTION OF INDTVIDUAL PATIENTS TO THE SPREAD OF INFECTION
Authors: W. C. NOBLE
Journal: British Journal of Dermatology (2006)

SUMMARY.— The existence of “dispersers” of micro-organisms in a skin hospital is described. There appeared to be no single factor, such as the diagnosis or the treatment received, which might be responsible, although all dispersers were amongst the most heavily colonized of patients. One strain of staphylococcus appeared to be selectively acquired by patients with mycosis fungoides.
JunD is a profibrogenic transcription factor regulated by Jun N-terminal kinase-independent phosphorylation
Authors: David E. Smart, Karen Green, Fiona Oakley, Jonathan B. Weitzman, Moshe Yaniv, Gary Reynolds, Jelena Mann, Harry Millward-Sadler, Derek A. Mann
Journal: Hepatology (2006)

JunD is implicated in the regulation of hepatic stellate cell (HSC) activation and liver fibrosis via its transcriptional regulation of the tissue inhibitor of metalloproteinases-1 (TIMP-1) gene. In the present study we found in vivo evidence of a role for JunD in fibrogenesis. Expression of JunD was demonstrated in alpha-SMA-positive activated HSCs of fibrotic rodents and human livers. The junD −/− mice were protected from carbon tetrachloride–induced fibrosis. The livers of injured junD −/− mice displayed significantly reduced formation of fibrotic crosslinked collagen and a smaller number of alpha-SMA-positive HSCs compared with those of wild-type (wt) mice. Hepatic TIMP-1 mRNA expression in injured junD −/− mice was 78% lower and in culture activated junD −/− HSCs was 50%-80% lower than that in wt mice. In examining the signal transduction mechanisms that regulate JunD-dependent TIMP-1 expression, we found a role for phosphorylation of the Ser100 residue of JunD but ruled out JNK as a mediator of this event, suggesting ERK1/2 is utilized. In conclusion , a signaling pathway for the development of fibrosis involves the regulation of TIMP-1 expression by phosphorylated JunD. Supplementary material for this article can be found on the HEPATOLOGY website (http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html).
Treating elderly patients with breast cancer.
Authors: S. M. Sagar, P. Lopez
Journal: BMJ (1992)

Preventing children drowning SIR,-Three summers ago our 2 year old son wandered from our garden.He was found in the swimming pool of a neighbouring garden: the pool was unfenced and had a non-weightsbearing cover.Resuscitation was not successful.We know of three families within 16 km who have suffered similar tragedies: as Alison Kemp and J R Sibert point out, drowning is a common cause of acci- dental death in children.'In our society, where there is increased emphasis on out ofdoors activities, the incidence ofdrowning in children will surely increase unless effective preventive measures are taken.We have written on this matter to our member of parliament without success.We support Kemp and Sibert's recom- mendations that domestic pools should have toddler proof fences with self locking doors and covers that can bear the weight of a child.People may argue that this interferes with personal liberty: the same was said about seat belt legislation.The children whose lives are at risk are too young to appreciate such abstract arguments.
Small-Cell Carcinoma of the Cervix: Fourteen Years of Experience at a Single Institution Using a Combined-Modality Regimen of Involved-Field Irradiation and Platinum-Based Combination Chemotherapy
Authors: P.J. Hoskins, K.D. Swenerton, J.A. Pike, P. Lim, C. Aquino-Parsons, F. Wong, N. Lee
Journal: Journal of Clinical Oncology (2003)

To determine the efficacy and toxicity of a combined-modality regimen of irradiation with platinum-based combination chemotherapy in small-cell carcinoma of the cervix (SCCC).Thirty-four patients with SCCC were seen and treated at the British Columbia Cancer Agency between May 1988 and November 2002. Two protocols were used, SMCC (May 1988 to December 1995) and SMCC2 (January 1996 to November 2002). Both protocols used cisplatin, etoposide, and involved-field irradiation (essentially pelvis plus or minus para-aortics) with concurrent chemotherapy. In addition, SMCC2 included carboplatin and paclitaxel, and the para-aortics were irradiated routinely.Thirty-one patients received either SMCC (n = 17) or SMCC2 (n = 14), and three patients did not (disease too extensive, n = 1; patient refusal, n = 1; and alternative regimen, n = 1). For the 31 patients treated on one of the protocols, the 3-year overall and failure-free survival (FFS) rates were 60% and 57%, respectively. The results were equivalent for SMCC and SMCC2. Radiologic stage was the only independent predictor for FFS (80% at 3 years for stage I and II patients v 38% at 3 years for stage III and IV patients). Distant failure (28%) was the most common cause of failure, with local failure occurring in 13% of patients. The switch to SMCC2 did not improve efficacy but did lessen the toxicity.SCCC can be successfully treated in approximately 55% of patients with a combination of irradiation and platinum-based chemotherapy. Disease extent predicts for chance of curability.
The importance of ontogenetic change in typical and atypical development
Authors: Tessa M. Dekker, Annette Karmiloff-Smith
Journal: Behavioral and Brain Sciences (2010)

Abstract The compelling case that Anderson makes for neural reuse and against modularity as organizing principle of the brain is further supported by evidence from developmental disorders. However, to provide a full evolutionary-developmental theory of neural reuse that encompasses both typical and atypical development, Anderson's “massive redeployment hypothesis” (MRH) could be further constrained by considering brain development across ontogeny.
Hypercalcaemia during resolution of calcinosis in juvenile dermatomyositis.
Authors: M L Wilsher, I M Holdaway, J D North
Journal: BMJ (1984)

Hypercalcaemia during resolution of calcinosis in juvenile dermatomyositisCalcinosis is common in juvenile dermatomyositis and may regress spontaneously.We report a case of juvenile dermatomyositis in which regression of pelvic calcinosis was associated with life threatening hypercalcaemia.
Pilot Clinical Trial of Hedgehog Pathway Inhibitor GDC-0449 (Vismodegib) in Combination with Gemcitabine in Patients with Metastatic Pancreatic Adenocarcinoma
Authors: Edward J. Kim, Vaibhav Sahai, Ethan V. Abel, Kent A. Griffith, Joel K. Greenson, Naoko Takebe, Gazala N. Khan, John L. Blau, Ronald Craig, Ulysses G. Balis, Mark M. Zalupski, Diane M. Simeone
Journal: Clinical Cancer Research (2014)

Abstract Purpose: The hedgehog (HH) signaling pathway is a key regulator in tumorigenesis of pancreatic adenocarcinoma and is upregulated in pancreatic adenocarcinoma cancer stem cells (CSCs). GDC-0449 is an oral small-molecule inhibitor of the HH pathway. This study assessed the effect of GDC-0449–mediated HH inhibition in paired biopsies, followed by combined treatment with gemcitabine, in patients with metastatic pancreatic adenocarcinoma. Experimental Design: Twenty-five patients were enrolled of which 23 underwent core biopsies at baseline and following 3 weeks of GDC-0449. On day 29, 23 patients started weekly gemcitabine while continuing GDC-0449. We evaluated GLI1 and PTCH1 inhibition, change in CSCs, Ki-67, fibrosis, and assessed tumor response, survival and toxicity. Results: On pretreatment biopsy, 75% of patients had elevated sonic hedgehog (SHH) expression. On posttreatment biopsy, GLI1 and PTCH1 decreased in 95.6% and 82.6% of 23 patients, fibrosis decreased in 45.4% of 22, and Ki-67 in 52.9% of 17 evaluable patients. No significant changes were detected in CSCs pre- and postbiopsy. The median progression-free and overall survival for all treated patients were 2.8 and 5.3 months. The response and disease control rate was 21.7% and 65.2%. No significant correlation was noted between CSCs, fibrosis, SHH, Ki-67, GLI1, PTCH1 (baseline values or relative change on posttreatment biopsy), and survival. Grade ≥3 adverse events were noted in 56% of patients. Conclusion: We show that GDC-0449 for 3 weeks leads to downmodulation of GLI1 and PTCH1, without significant changes in CSCs compared with baseline. GDC-0449 and gemcitabine were not superior to gemcitabine alone in the treatment of metastatic pancreatic cancer. Clin Cancer Res; 20(23); 5937–45. ©2014 AACR.
Combined Inhibition of PLCγ-1 and c-Src Abrogates Epidermal Growth Factor Receptor–Mediated Head and Neck Squamous Cell Carcinoma Invasion
Authors: Hiroshi Nozawa, Gina Howell, Shinsuke Suzuki, Qing Zhang, Yanjun Qi, Judith Klein-Seetharaman, Alan Wells, Jennifer R. Grandis, Sufi M. Thomas
Journal: Clinical Cancer Research (2008)

Abstract Purpose: Mortality from head and neck squamous cell carcinoma (HNSCC) is usually associated with locoregional invasion of the tumor into vital organs, including the airway. Understanding the signaling mechanisms that abrogate HNSCC invasion may reveal novel therapeutic targets for intervention. The purpose of this study was to investigate the efficacy of combined inhibition of c-Src and PLCγ-1 in the abrogation of HNSCC invasion. Experimental Design: PLCγ-1 and c-Src inhibition was achieved by a combination of small molecule inhibitors and dominant negative approaches. The effect of inhibition of PLCγ-1 and c-Src on invasion of HNSCC cells was assessed in an in vitro Matrigel-coated transwell invasion assay. In addition, the immunoprecipitation reactions and in silico database mining was used to examine the interactions between PLCγ-1 and c-Src. Results: Here, we show that inhibition of PLCγ-1 or c-Src with the PLC inhibitor U73122 or the Src family inhibitor AZD0530 or using dominant-negative constructs attenuated epidermal growth factor (EGF)–stimulated HNSCC invasion. Furthermore, EGF stimulation increased the association between PLCγ-1 and c-Src in HNSCC cells. Combined inhibition of PLCγ-1 and c-Src resulted in further attenuation of HNSCC cell invasion in vitro. Conclusions: These cumulative results suggest that PLCγ-1 and c-Src activation contribute to HNSCC invasion downstream of EGF receptor and that targeting these pathways may be a novel strategy to prevent tumor invasion in HNSCC.