tbuckley/cabot-search huggingface dataset:
Abstract 4452: Regulation of histone deacethylase 8 expression by inhibitory GTP binding proteins signaling via JNK pathway in H1299 non-small-cell lung cancer cells
Authors: Hwa-jeong Hahn, Yong-Sung Juhnn
Journal: Cancer Research (2014)

Abstract Histone deacetylases (HDACs) have been reported to regulate the cell growth, proliferation, and apoptosis, and HDAC8 seems to be involved in tumor growth and progression. Inhibitory heterotrimeric GTP-binding proteins (Gαi) are activated by seven transmembrane receptors, and regulate cell proliferation by regulating the activity of adenylate cyclase, phospholipase C beta, and ion channels. This study investigated the effects of Gαi signaling system on the expression of HDAC8 and the underlying mechanism in lung cancer cells. When Gi signaling system was activated by expressing Gαi1Q204L, a constitutively active mutant Gαi1, the expression of HDAC8 protein and mRNA was increased in H1299 non-small-cell lung cancer (NSCLC) cells. Inhibition of transcription by treatment with Actinomycin D did not block the increase in mRNA, and the degradation of HDAC8 mRNA was facilitated in Gαi1Q204L -expressing cells, suggesting Gαi1 signaling system increases HDAC8 expression by stabilizing mRNA. In addition, treatment with adenosine or an adenosine receptor agonist, CCPA (2-chloro-N(6)-cyclopentyladenosine), which activate Gαi-coupled receptors, also increased HDAC8 mRNA and protein levels. The effect of adenosine was blocked by treatment with DPCPX (8-Cyclopentyl-1,3-dipropylxanthine), an adenosine A1 receptor antagonist. JNK phosphorylation was inhibited by treatment with the adenosine and CCPA. Treatment with a JNK inhibitor, SP600125 increased HDAC8 expression. Adenosine inhibited the activation of MKK4 and CDC 42, which act upstream of JNK. Activation of Gαi signaling stimulated proliferation of H1299 cells, and the stimulating effect was abolished by knockdown of Gαi1. Thus, we conclude that Gαi signaling increases HDAC8 expression via JNK pathway, resulting in stimulation of cell proliferation of H1299 lung cancer cells. This study suggests that Gαi signaling may be involved in epigenetic control of gene expression by regulating HDAC8 expression. Citation Format: Hwa-jeong Hahn, Yong-Sung Juhnn. Regulation of histone deacethylase 8 expression by inhibitory GTP binding proteins signaling via JNK pathway in H1299 non-small-cell lung cancer cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4452. doi:10.1158/1538-7445.AM2014-4452
Reflections at a Palliative Care Unit
Authors: Steven J. McPhee
Journal: JAMA (2002)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Assisted Suicide Compared with Refusal of Treatment: A Valid Distinction?
Authors: Franklin G. Miller, Joseph J. Fins, Lois Snyder
Journal: Annals of Internal Medicine (2000)

The continuing debate over the deeply controversial issue of physician-assisted suicide has been complicated by confusion about how this practice resembles or differs from refusal of life-sustaining treatment. Perspectives on ethics and policy hinge on the contested issue of whether a valid distinction can be made between assisted suicide and withdrawal of treatment. This paper uses three illustrative cases to examine leading arguments for and against the recognition of a fundamental distinction between these practices. The first case involves assisted suicide by ingestion of prescribed barbiturates, the second involves withdrawal of artificial nutrition and hydration, and the third involves a decision to stop eating and drinking. On theoretical and practical grounds, this paper defends the position that there is a valid distinction between assisted suicide and refusal of treatment.
The Origins of the World Health Organization: A Personal Memoir, 1945-1948
Authors: Swailem S. Hennein
Journal: JAMA (1983)

This short monograph is an account of what took place behind the scenes from the time the World Health Organization was conceived as an idea in 1945 until its birth in 1948. The author, a Chinese physician (known for the important role he played during those formative years), was then on the staff of the United Nations' Relief and Rehabilitation Administration. Subsequently, he filled several key positions and, with a missionary zeal, took up the cause of the WHO. Being one of the few charter participants still living, he felt impelled to open his diary and share with the reader his recollections, thereby filling a gap in knowledge and setting records straight. The idea of a single health organization that was to be part of the UN framework and at the same time, independent of it was difficult to sell. Nevertheless, it has proved extremely foresighted, for, against all odds,
Histologic Predictors of Fibrosis Progression in Liver Allografts in Patients With Hepatitis C Virus Infection
Authors: Zina Meriden, Kimberly A. Forde, Theresa L. Pasha, Jia–Ji Hui, K. Rajender Reddy, Emma E. Furth, Rebecca G. Wells
Journal: Clinical Gastroenterology and Hepatology (2009)
Suivi prospectif de 100 panniculites mésentériques diagnostiquées au scanner abdomino-pelvien: quel suivi pour nos patients?
Authors: C Briquez, L Vuitton, N Badet, M Lavy, S Valmary-Degano, AL Parmentier, B Heyd, F Mauny, S Koch, E Delabrousse
Journal: Endoscopy (2014)

La panniculite mésentérique (PM) est un processus inflammatoire aspécifique affectant le pédicule adipeux du mésentère, asymptomatique ou parfois révélé par de la fièvre, des douleurs abdominales, des troubles du transit ou des vomissements. Son diagnostic est scannographique et se défini par une augmentation de la densité de la graisse mésentérique appelée « misty mesentery » associée à des nodules tissulaires au sein de cette infiltration. Une pseudocapsule hyperdense qui circonscrit le mésentère inflammatoire et/ou un halo graisseux autour des vaisseaux et des nodules appelé « fat ring sign » peuvent également être présents. Des études antérieures suggèrent une association entre la PM et des pathologies néoplasiques, notamment le lymphome, mais aussi des affections abdominales aigues, des pathologies infectieuses ou inflammatoires. Cependant les données sont hétérogènes et majoritairement rétrospectives. Finalement, la prise en charge immédiate et le suivi des patients sont mal codifiés. L'objectif de notre étude était de connaître la prévalence des cancers chez les patients présentant une PM au scanner abdominopelvien, et d'étudier l'évolution clinique et radiologique chez les patients.
Existence and quality of written antenatal screening policies in the United Kingdom: postal survey
Authors: B. Lane
Journal: BMJ (2001)

The assessment of antenatal care of women at increased risk of having a baby with Down's syndrome, neural tube defect, thalassaemia, and cystic fibrosis that was undertaken by the national confidential inquiry into counselling by non-geneticists revealed several problems, including poor record keeping.1 These problems were reported to the Department of Health in five specific papers and four summary, peer reviewed papers and on the internet.2 The Royal College of Obstetricians and Gynaecologists then issued guidelines recommending that antenatal units have written policies for screening for Down's syndrome and for neural tube defect and that haemoglobinopathy screening be offered to all patients whose ethnic origin makes them susceptible.3 There is less consensus on screening for cystic fibrosis (UK National Screening Committee, joint meeting of the antenatal and child health screening subgroups, cystic fibrosis workshop, London, 2 June 1999). We assessed the response among antenatal staff to the outcomes of …
Gentamicin in Ascitic Fluid
Authors: SHIV K. SETH
Journal: Annals of Internal Medicine (1979)

Letters and Corrections1 July 1979Gentamicin in Ascitic FluidSHIV K. SETH, PH.D., TIMOTHY D. MOORE, B. PHARM.SHIV K. SETH, PH.D.Search for more papers by this author, TIMOTHY D. MOORE, B. PHARM.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-91-1-134_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: We wish to point out a potential hazard incurred with gentamicin therapy not addressed in the review article on gentamicin by Appel and Neu in the October 1978 issue (1).Concentrations of antibiotics in ascitic fluid have been shown to be adequate in patients with bacterial peritonitis who receive systemic therapy (2, 3). Because information on transperitoneal movement of antibiotics is limited, drug dosage and route of administration in such patients are usually chosen empirically. The following case presents an unusual gentamicin dosing problem.A 63.2-kg 45-year-old black man with a long history of alcohol abuse presented...References1. APPELNEU GH. Gentamicin in 1978. Ann Intern Med. 1978;89:528-38. LinkGoogle Scholar2. WILSONCHALMERSMADATT DTM. The passage of cephalothin into and out of ascitic fluid. Am J Med Sci. 1967;253:449-52. CrossrefMedlineGoogle Scholar3. GERDINGWENDELLSCHIERL DHE. Antibiotic concentrations in ascitic fluid of patients with ascities and bacterial peritonitis. Ann Intern Med. 1977;86:708-13. LinkGoogle Scholar4. CHANBENNERHOEPRICH REP. Gentamicin therapy in renal failure: A nomogram for dosage. Ann Intern Med. 1972;76:773-8. LinkGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: The Ohio State University Hospitals Columbus, OH 43210 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByPeritoneal fluid concentrations of gentamicin in patients with spontaneous bacterial peritonitis 1 July 1979Volume 91, Issue 1Page: 134-135KeywordsAlcoholismAntibioticsAttentionDrug administrationDrugsPeritonitisRoutes of administration Issue Published: 1 July 1979 PDF DownloadLoading ...
Case 18-1983
Authors: Richard C. Cabot, Robert E. Scully, Eugene J. Mark, Betty U. McNeely, Philip C. Amrein, Nancy L. Harris
Journal: New England Journal of Medicine (1983)

Presentation of Case First admission. A 24-year-old man was admitted to the hospital because of fever.The patient was malformed and mildly mentally retarded from birth; neurologic evaluation elsewhere led to a diagnosis of cerebral palsy. He resided with his family and attended a special school. Thirty-three months before admission a grand-mal seizure occurred; medical evaluation resulted in the diagnosis of end-stage renal disease of uncertain cause. Peritoneal dialysis was performed, and the urea nitrogen fell from 161 mg to 65 mg per 100 ml (57.5 mmol to 23 mmol per liter). An arteriovenous fistula was constructed in the . . .
INVESTIGATION RELATIVE TO B. WELCHII INFECTION OF THE INTESTINAL TRACT AS THE ETIOLOGICAL FACTOR IN PERNICIOUS ANEMIA 1
Authors: Robert N. Nye
Journal: Journal of Clinical Investigation (1927)

This work was originally undertaken with the purpose of confirming Herter's (1) and Simonds' (2) observations on the prevalence of B. Welchii in the stools of patients with pernicious anemia.In view of the toxin produced by all strains, the marked hemolytic properties of many strains of B. Welchii and the presence of anemia associated with B. Welchii infection as reported by Klotz and Holman (3) and of hemoglobinuria as noted by Herter (1) and by Schumm (4), chronic infections with B. Welchii of some portion of the gastro-intestinal tract with consequent absorption of hemolysins seemed to offer an attractive and quite plausible etiology for the disease, pernicious anemia.The possibility of intestinal absorption as an etiological fac- tor in pernicious anemia was seriously considered a number of years ago by Hunter (5), and more recently the idea has been mentioned by van der Reis (6) and upheld by Seyderhelm (7).Within the past year several articles have appeared which correlate B. WdIchii with pernicious anemia.Kahn (8) examined bacteriologically the stools from patients suffering from various diseases in which chronic intestinal infection was a possible etiological factor, and re- ports as the only consistent finding the presence of considerable num- bers of B. Welchii spores and the absence of spores of the other common anaerobes in all the stools from cases of pernicious anemia.Moench, Kahn and Torrey (9) write in more detail on the bacteriological ex- amination of 72 stool specimens from 33 cases of pernicious anemia.They found uniformly high B. coli counts and B. Welchii spore counts
Sniffing Up Trouble: Inhalation of Volatile Substances-Reply
Authors: William G. Troutman
Journal: JAMA (1985)

<h3>In Reply.—</h3> We acknowledge the interest of the writers and their corporation in the problems posed by inhalation of manufactured substances. Their action to include mustard oil in the preparation of typewriter correction fluid should prove extremely effective in deterring abuse of this product. The industry's efforts in development of the Solvent Abuse Foundation for Education should be encouraged. It is through public awareness of these potential dangers that responsible attitudes can be developed. The conclusions and opinions contained in our article recently published inThe Journal<sup>1</sup>were based on the data collected under the jurisdiction of the New Mexico Office of the Medical Investigator. Drs Greer and Giovacchini refer to data contained in an article that was collected from a different geographic population and from sources lacking any standardized method of identification of such cases.<sup>2</sup>Nevertheless, when one examines the specific agents identified in the various categories,
Ductal Carcinoma in Situ — The Focus for Prevention, Screening, and Breast Conservation in Breast Cancer
Authors: David L. Page, Jean F. Simpson
Journal: New England Journal of Medicine (1999)

We entered this century with a confusion of terms for benign conditions of the breast that are possibly associated with cancer, summarized by J.C. Warren in 1905.1 We exit this century with a broad consensus that largely fulfills Warren's call for consistent terminology, not only for pathologists and surgeons but also for other specialists involved in the diagnosis and treatment of breast disease. We now have general agreement about criteria with respect to histologic diagnoses, epidemiologic associations, and prognostic implications. The various subtypes of the group of conditions known as ductal carcinoma in situ are the pivotal conditions between benign . . .
Prediction of Medium-Term Outcome in Cluster B Personality Disorder following Residential and Outpatient Psychosocial Treatment
Authors: Marco Chiesa, Peter Fonagy
Journal: Psychotherapy and Psychosomatics (2007)

&lt;i&gt;Background:&lt;/i&gt; There is a paucity of research concerning the identification of individual characteristics predictive of outcome in the treatment of personality disorders (PDs). &lt;i&gt;Methods:&lt;/i&gt; In this study, we carried out a predictor analysis of a sample of 73 hospitalized patients with a primary diagnosis of cluster B PD admitted to two different psychosocial programs for PD: (a) long-term inpatient treatment, and (b) a step-down program. &lt;i&gt;Results:&lt;/i&gt; Younger age, higher Global Assessment Scale intake scores, longer length of treatment, absence of self-mutilation and avoidant PDs were a significant predictor of outcome at 24-month follow-up. Self-harming patients allocated to the step-down program had higher rates of improvement compared with patients allocated to the long-term inpatient model. &lt;i&gt;Conclusions:&lt;/i&gt; The findings may carry potential clinical implications concerning patient selection and treatment delivery for inpatient and outpatient psychosocial programs for cluster B PD. Limitations include a relatively low sample size for a regression analysis, and a larger sample of cluster B patients may be needed to ensure greater reliability of results.
PARTIAL OCCLUSION OF THE AORTA WITH SILK SUTURES, AND COMPLETE OCCLUSION WITH FASCIAL PLUGS. THE EFFECT OF LIGATURES ON THE ARTERIAL WALL
Authors: Mont R. Reid
Journal: The Journal of Experimental Medicine (1924)

1. Partial occlusion of the aorta of dogs may be produced by Halsted mattress sutures of silk. This occlusion persists. 2. A method of completely occluding the aorta of dogs by means of anchoring fascial plugs in the lumen is described. 3. The vessel wall below and at the site of an occluding fascial plug undergoes marked atrophy. 4. Ligatures used for ligating or plicating large arteries cause death of the arterial wall. The same is true for encircling metallic bands. 5. Catgut is dangerous to use for the ligation of large arteries, since it may give way before the necrosed vessel wall becomes completely substituted by fibrous tissue.
Assessment of mutations in the best macular dystrophy (VMD2) gene in patients with adult-onset foveomacular vitelliform dystrophy, age-related maculopathy, and bull's-eye maculopathy
Authors: Johanna M Seddon, Mehran A Afshari, Sanjay Sharma, Paul S Bernstein, Sandy Chong, Amy Hutchinson, Konstantin Petrukhin, Rando Allikmets
Journal: Ophthalmology (2001)
CXCR2 and CXCR4 antagonistically regulate neutrophil trafficking from murine bone marrow
Authors: Kyle J. Eash, Adam M. Greenbaum, Priya K. Gopalan, Daniel C. Link
Journal: Journal of Clinical Investigation (2010)

Neutrophils are a major component of the innate immune response. Their homeostasis is maintained, in part, by the regulated release of neutrophils from the bone marrow. Constitutive expression of the chemokine CXCL12 by bone marrow stromal cells provides a key retention signal for neutrophils in the bone marrow through activation of its receptor, CXCR4. Attenuation of CXCR4 signaling leads to entry of neutrophils into the circulation through unknown mechanisms. We investigated the role of CXCR2-binding ELR+ chemokines in neutrophil trafficking using mouse mixed bone marrow chimeras reconstituted with Cxcr2(-/-) and WT cells. In this context, neutrophils lacking CXCR2 were preferentially retained in the bone marrow, a phenotype resembling the congenital disorder myelokathexis, which is characterized by chronic neutropenia. Additionally, transient disruption of CXCR4 failed to mobilize Cxcr2(-/-) neutrophils. However, neutrophils lacking both CXCR2 and CXCR4 displayed constitutive mobilization, showing that CXCR4 plays a dominant role in neutrophil trafficking. With regard to CXCR2 ligands, bone marrow endothelial cells and osteoblasts constitutively expressed the ELR+ chemokines CXCL1 and CXCL2, and CXCL2 expression was induced in endothelial cells during G-CSF-induced neutrophil mobilization. Collectively, these data suggest that CXCR2 signaling is a second chemokine axis that interacts antagonistically with CXCR4 to regulate neutrophil release from the bone marrow.
Hyperbaric Treatment of Cerebral Air Embolism During Cardiopulmonary Bypass
Authors: Peter M. Winter
Journal: JAMA (1971)

Hyperbaric treatment was used in three patients with extensive neurologic damage from massive cerebral air embolism occurring during cardiopulmonary bypass. Exposure to high pressure (6 atmospheres absolute) was followed by prolonged decompression over a period of 38 hours. All patients began to respond during compression, and improvement continued later. In two patients eventual recovery was complete, and there were no signs of cerebral damage. The third patient may yet make a full recovery.
Hierarchical and cybernetic nature of biologic systems and their relevance to homeostatic adaptation to low-level exposures to oxidative stress-inducing agents.
Authors: J E Trosko
Journal: Environmental Health Perspectives (1998)

During evolution in an aerobic environment, multicellular organisms survived by adaptive responses to both the endogenous oxidative metabolism in the cells of the organism and the chemicals and low-level radiation to which they had been exposed. The defense repertoire exists at all levels of the biological hierarchy--from the molecular and biochemical level to the cellular and tissue level to the organ and organ system level. Cells contain preventive antioxidants to suppress oxidative damage to membranes. Cells also contain proteins and DNA; built-in redundancies for damaged molecules and organelles; tightly coupled redox systems; pools of reductants; antioxidants; DNA repair mechanisms and sensitive sensor molecules such as nuclear factor kappa beta; and signal transduction mechanisms affecting both transcription and post-translational modification of proteins needed to cope with oxidative stress. The biologic consequences of the low-level radiation that exceeds the background level of oxidative damage could be necrosis or apoptosis, cell proliferation, or cell differentiation. These effects are triggered by oxidative stress-induced signal transduction mechanisms--an epigenetic, not genotoxic, process. If the end points of cell proliferation, differentiation, or cell death are not seen at frequencies above background levels in an organism, it is unlikely that low-level radiation would play a role in the multistep processes of chronic diseases such as cancer. The mechanism linked to homeostatic regulation of proliferation and adaptive functions in a multicellular organism could provide protection of any one cell receiving deposited energy by the radiation tract through the sharing of reductants and by triggering apoptosis of target stem cells. Examples of the role of gap junctional intercellular communication in the adaptive response of cells and the bystander effect illustrate how the interaction of cells can modulate the effect of radiation on the single cell.