tbuckley/cabot-search huggingface dataset:
493. High Efficiency Gene Targeting to Mammalian Joint Tissue Using Self- Complementary Adeno-Associated Viral Vector Serotypes
Authors: Laurie R. Goodrich, Vivian W. Choi, Beth C. DudaCarbone, C. Wayne McIlwraith, Richard J. Samulski
Journal: Molecular Therapy (2006)

Introduction: Gene therapy for joint diseases relies on a non-immunogenic gene delivery vector that can efficiently and persistently transduce joint specific tissues (eg. chondrocytes and synoviocytes). Recombinant adeno-associated virus (rAAV) is an emerging and promising vector for joint diseases due to its potential to efficiently express therapeutic genes for long periods of time and its purported low incidence of immune reactions and cell toxicity. With the increasing availability of different AAV-serotype vectors for tissue targeting, we investigated the best AAV serotype to deliver a self-complementary AAV (scAAV) genome to chondrocytes and synoviocytes for future gene therapy applications in joint diseases. Materials and methods: Chondrocytes and synoviocytes were harvested from joints of immature horses. Cell monolayers were cultured in a chondrogenic media. Two days after seeding, various scAAV vector serotypes carrying a GFP expression cassette were used to infect cells in a dose-response manner. Fluorescence intensity was measured using a fluorometer and the number of transduced cells was measured by fluorescent microscope daily for a total of 10 weeks. Cell viability was determined using Trypan blue and vector toxicity was measured by quantitative PCR and relative gene expression of equine MMP-1, MMP-3, MMP-13 and Aggrecanase. Results: Our results demonstrated that chondrocytes had a transduction efficiency of 85% at day 3 post-infection with a particular scAAV serotype, as compared to 70%, 65%, 50%, 20% and <20% for other serotypes of scAAV, respectively. In addition, prolonged gene expression was achieved with over 50% of chondrocytes remaining GFP-positive 6 weeks post-infection, which has not been previously demonstrated by other vectors used for this purpose. Similar results were obtained for synoviocytes as over 80% of cells remained successfully transduced by a specific scAAV serotype two weeks post infection and 40-50% of cells continued to express transgene 6 weeks later. Cell viability of both chondrocytes and synoviocytes was determined to be over 80% throughout the course of the experiment and there were no elevations in expression of inflammatory molecules detected for the optimal serotypes determined. Discussion: We established the use of specific scAAV serotypes for efficient tissue targeting with persistent transgene expression on mammalian chondrocytes and synoviocytes, which enhances the likelihood of successful gene therapy for joint diseases such as osteoarthritis and rheumatoid arthritis. Further investigation on animal models and clinical applications of our system will be developed for therapeutic uses.
Making a stronger case for comparative research to investigate the behavioral and neurological bases of three-dimensional navigation
Authors: Daniele Nardi, Verner P. Bingman
Journal: Behavioral and Brain Sciences (2013)

Abstract The rich diversity of avian natural history provides exciting possibilities for comparative research aimed at understanding three-dimensional navigation. We propose some hypotheses relating differences in natural history to potential behavioral and neurological adaptations possessed by contrasting bird species. This comparative approach may offer unique insights into some of the important questions raised by Jeffery et al.
Low Prevalence of Diabetic Retinopathy in a Chinese Population
Authors: Ming-Xia Yuan, Zhi-Hui Peng, Zhong Xin, Jian-Ping Feng, Lin Hua, Jing Shi, Kun Geng, Zhi-Xin Xu, Xiao-Rong Zhu, Xi Cao, Chang Liu, Jin-Kui Yang
Journal: Diabetes Care (2012)

As a typical microvascular complication and a leading cause of blindness in working-age individuals, diabetic retinopathy (DR) is a serious threat to the quality of life for millions worldwide. The prevalence of diabetic retinopathy is 22–37% in individuals with known diabetes (1,2) and 6–13% in individuals with newly diagnosed diabetes (3). However, current estimates of the prevalence and risk factors for DR are mostly derived from studies of Western populations. Population-based data on DR in Asians remain limited.

It has been hypothesized that ethnic differences may result in varying susceptibilities to diabetic microvascular complications (4). For example, Greek ethnicity may confer some protection against DR (5).

From July 2010 through March 2011, the 2010 Health Examination Survey in Beijing, a …
Kinetics of serological responses in influenza A(H7N9)-infected patients correlate with clinical outcome in China, 2013
Authors: A Zhang, Y Huang, D Tian, E H Lau, Y Wan, X Liu, Y Dong, Z Song, X Zhang, J Zhang, M Bao, M Zhou, S Yuan, J Su, Z Zhu, Y Hu, L Chen, C Y Leung, J T Wu, Z Zhang, X Zhang, J S Peiris, J Xu
Journal: Eurosurveillance (2013)

Binary file ES_Abstracts_Final_ECDC.txt matches
Tidal ventilation at low airway pressures can augment lung injury.
Authors: J G Muscedere, J B Mullen, K Gan, A S Slutsky
Journal: American Journal of Respiratory and Critical Care Medicine (1994)

Intermittent positive pressure ventilation with large tidal volumes and high peak airway pressures can result in pulmonary barotrauma. In the present study, we examined the hypothesis that ventilation at very low lung volumes can also worsen lung injury by repeated opening and closing of airway and alveolar duct units as ventilation occurs from below to above the infection point (Pinf) as determined from the inspiratory pressure-volume curve. We ventilated isolated, nonperfused, lavaged rat lungs with physiologic tidal volumes (5 to 6 ml/kg) at different end-expiratory pressures (above and below Pinf) and studied the effect on compliance and lung injury. In the groups ventilated with positive end-expiratory pressure (PEEP) below Pinf compliance fell dramatically after ventilation. It did not change in either the control group or the group ventilated with PEEP above Pinf. Lung injury assessed morphologically was significantly greater in the groups ventilated with a PEEP below Pinf, and in these groups the site of injury was dependent on the level of PEEP. The group ventilated without PEEP had significantly greater respiratory and membranous injury to bronchioles, while the group ventilated with PEEP of 4 cm H2O had significantly greater alveolar duct injury. In conclusion, ventilation at lung volumes below those found at Pinf caused a significant decrease in lung compliance and progression of lung injury. Therefore, in addition to high airway pressures, end-expiratory lung volume is an important determinant of the degree and site of lung injury during positive-pressure ventilation.
Pancreaticojejunostomy by reinforcing the pancreas without covering the anastomotic line reduces pancreatic fistula
Authors: Masafumi Kuramoto, Satoshi Ikeshima, Shinya Shimada, Kenichiro Yamamoto, Toshiro Masuda, Kenichi Nakamura, Toichiro Takeguchi, Hideo Baba
Journal: International Journal of Surgery (2013)
Stop the silent misdiagnosis: patients' preferences matter
Authors: A. G. Mulley, C. Trimble, G. Elwyn
Journal: BMJ (2012)

Correct treatment recommendations require accurate diagnosis not only of the medical condition but of patients' treatment preferences. <b>Al Mulley</b>, <b>Chris Trimble</b>, and <b>Glyn Elwyn </b>outline how to ensure that preferences are not misdiagnosed
Treating stroke: home or hospital?
Authors: B Isaacs
Journal: BMJ (1978)

11 November, p 1321) rightly draw attention to the need to evaluate claims for the effectiveness of stroke rehabilitation in hospital.However, I would challenge their statement that stroke rehabilitation units "make great demands on the health service in both money and staff."It is at least arguable that the demands arise not from the units but from the needs of the severely disabled patients, who require a great deal of care wherever they are located.Indeed, these needs may well be met more economically and more effectively when the patients are grouped in a unit dedicated to multidisciplinary rehabilitation than when they are scattered throughout the hospital.The 30-bedded stroke rehabilitation ward at Lightburn Hospital, Glasgow, obtained good results' in the rehabilitation of severely disabled stroke patients with no more medical and nursing staff than in the geriatric wards of the hospital and with the part-time services of only two physiotherapists, one occupational
Educating Australian physiotherapists: striving for excellence in sport and exercise medicine
Authors: Kim Bennell, Gillian Webb
Journal: British Journal of Sports Medicine (2000)

Physiotherapists play a major role in sports medicine delivery and research in Australia, a country renowned for its sporting achievements and sporting culture. Sports medicine in this country is a relatively young specialty which has undergone rapid development particularly since the 1956 Melbourne Olympic Games. Educating physiotherapists in sports and exercise begins in undergraduate training where a large proportion of the physiotherapy course focuses on theoretical knowledge and practical skills in the neuromusculoskeletal area. After graduation, further sports physiotherapy experience can be gained through formal postgraduate university qualifications or a variety of professional development activities.

The first formal education programme for students in the area of physiotherapy began in Australia at the University of Melbourne in the early 1900s. Initially, registration to practice physiotherapy in Australia was licensed under the Massage Registration Board and the Massage Act. In the 1970s, under the auspices of the Physiotherapy Registration Board, physiotherapists in Australia were granted primary contact practitioner status. This was a first in the world for physiotherapy. The responsibility associated with primary practitioner status had a profound effect on undergraduate education requiring an emphasis on differential diagnosis by way of clinical reasoning and clinical decision making.

In Australia in the year 2000, seven universities offer physiotherapy undergraduate programmes with about 650 students in total graduating each year. Two more programmes have started and will have graduates in the near future. A further university has advertised for a new chair of physiotherapy, and other universities are considering establishing programmes. This is a clear demonstration of the popularity of physiotherapy as a choice of career for Australian students. In particular, many students are interested in the area of sports physiotherapy. The programmes are very competitive to enter and the students entering the programmes come from the top 2–5% of secondary school leavers. There …
Abstract 3278: Novel knock-in mice expressing human/mouse chimeric endoglin (ENG; CD105) develop normally and will be valuable for evaluating clinical application of anti-human ENG antibodies
Authors: Hirofumi Toi, Jill Duzen, Kanako Fujita, Masanori Tsujie, Hilda Tsai, Ben K. Seon
Journal: Cancer Research (2011)

Abstract The major objective of this study is to develop a novel animal model to facilitate optimal clinical application of anti-human ENG (huENG) monoclonal antibodies (mAbs) to treat cancer patients. ENG is a proliferation-associated homodimeric cell membrane antigen of endothelial cells and leukemia cells. It is strongly expressed on angiogenic vascular endothelium in tumors and also expressed in lymphatic vessels in tumors. Furthermore, ENG is a TGF-β coreceptor and essential for angiogenesis/vascular development. Mice lacking ENG die from defective vascular development. Mutations in the huENG gene are associated with hereditary hemorrhagic telangiectasia type 1 (HHT1) which is characterized by vascular malformations and recurrent bleeding. huENG shows a relatively restricted expression pattern in tissues and cells. We hypothesized that huENG is a safe and effective target in vascular-targeting antiangiogenic therapy (VTAT). Clinical trials of a chimeric anti-huENG mAb c-SN6j (also known as TRC105) are in progress (ClinicalTrials.gov Identifier: NCT00582985 and NCT01090765). The major obstacle in animal model studies of anti-huENG mAbs is that most anti-huENG mAbs do not react with mouse ENG or mouse endothelial cells. To overcome this obstacle, we generated knock-in mice expressing human/mouse chimeric ENG. We designed the chimeric vectors to contain huENG gene segments of desired location and length so that the expressed huENG protein contains desired epitopes. Nevertheless, the major concern was that the generated knock-in mice might suffer from defective vascular development or HHT-like symptoms if the chimeric ENG does not function properly in mice. We generated two types of knock-in mice containing different sizes of huENG gene. In both cases, mice develop normally and show no sign of HHT-like symptoms in the homozygous mice. Tumors of 4T1 breast cancer cells and Colon26 colon cancer cells were generated in the knock-in mice to test expression of chimeric ENG protein in the tumor microvessels. Immunohistochemical staining of the tumors using several anti-huENG mAbs showed that the tumor microvessels express desired epitopes of huENG protein which are candidate targets for VTAT. The epitopes defined by these mAbs have been mapped. This novel knock-in mouse model will be useful for evaluating antitumor efficacy of many anti-huENG mAbs. In addition, this model will be useful for evaluating appropriate combination of an anti-huENG mAb with other anticancer agents to achieve optimal antitumor efficacy. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3278. doi:10.1158/1538-7445.AM2011-3278
224 REMARKABLE INCREASE IN CYP24A1 MRNA LEVEL AFTER 1,25-DIHYDROXYVITAMIN D3 TREATMENT OF HEPATOCELLULAR CARCINOMA CELL LINES
Authors: E. Horvath, P. Lakatos, B. Balla, J. Kosa, I. Kovalszky, F. Szalay
Journal: Journal of Hepatology (2011)
Points: What is "serum albumin"?
Authors: I B Holbrook
Journal: BMJ (1986)

SIR,-The president of the Hospital Consultants and Specialists Association states (18 January, p 206) that his association "was very surprised to read the evidenqe of the Short select, committee given by the spokesmen for the medical profession, including the consultant representative on, the BMA delegation, and felt obliged to conduct a survey to.find out if there had been a change of attitude to the hospital staffing structure."As'the "consultant representative" referred to above, I should like to put the-record straight.In 1982 the Central Committee for Hospital Medical Services supported the concept of a controlled.expansion of consultants in general surgery, general medicine, and obstetrics and gynaecology; a reduction in the number of UK graduate registrars in these specilties; and the introduction of an overseas doctors training scheme.These proposals were put to the con- sultant body t large, and 85% of the m,any respondents supported these proposals.This information was included in the select conputtee's *minutes of evidence for Wednesday 27 March 1985, to which the president of the HCSA referred in his letter.Indeed, it states quite clearly i'n those minutes (p 6, paragraph 16) that " HC:MS did not support the Social Servies Committee's geieral notion of a consultant provided service."-In oral evidence used the-phrase "consultant based," and from the subsequent paragraphs itis clear that I was using the phrase.todescribe the proposals tharhad been accepted by the profession in 1982.This includes an adequate numnber of
Epidemiology and the 1980 Census
Authors: MATTHEW M. ZACK
Journal: Annals of Internal Medicine (1980)

Editorials1 April 1980Epidemiology and the 1980 CensusMATTHEW M. ZACK JR., M.D., CLARK W. HEATH JR., M.D.MATTHEW M. ZACK JR., M.D.Search for more papers by this author, CLARK W. HEATH JR., M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-92-4-562 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe constitutional purpose of the first decennial Census in 1790 was to provide states with a basis for fair apportionment among seats in the U.S. House of Representatives. Although this primary purpose has not changed, new needs for information about the nation's population have developed as the country has grown. The first Census asked five questions of 4 million U.S. residents. This month, the 20th decennial Census of population and housing will be asking 19 questions of an estimated 222 million U.S. residents in 80 million households, with a sample of the latter being asked 46 additional questions. The answers...References1. MACMAHONPUGH BT. Epidemiology: Principles and Methods. Boston: Little, Brown and Co.; 1970. Google Scholar2. MASONMCKAYHOOVERBLOTFRAUMENI TFRWJ. Atlas of Cancer Mortality for U.S. Counties: 1950-1969. Washington, D.C.: U.S. Government Printing Office; 1975. (DHEW Publication No. (NIH) 75-780). Google Scholar3. HOLLANDKARHAUSENWAINWRIGHT WLA eds. Health Care and Epidemiology. Boston: G. K. Hall and Co.; 1979. Google Scholar4. . Smoking and Health: a Report of the Surgeon General. Rockville, Maryland: United States Department of Health, Education, and Welfare; 1979; (DHEW Publication No. (PHS) 79-50066). Google Scholar5. . Healthy People: the Surgeon General's Report on Health Promotion and Disease Prevention, 1979. Washington, D.C.: U.S. Government Printing Office; 1979. (DHEW Publication No. (PHS) 79-55071). Google Scholar6. . Current Estimates from the Health Interview Survey: United States—1978. Hyattsville, Maryland: National Center for Health Statistics; 1979. (Vital and Health Statistics. Series 10: Data from the National Health Survey, No. 130) (DHEW Publication No. (PHS) 80-1551). Google Scholar7. HARRINGTONBLOTHOOVERHOUSWORTHHEATHFRAUMENI JWRWCJ. Lung cancer in coastal Georgia: a death certificate analysis of occupation. J Natl Cancer Inst. 1978;60:295-8. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Chronic Diseases Division Bureau of Epidemiology Center for Disease Control Atlanta, Georgia PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByCore Competencies in Geriatric Dentistry Fellowship Programs: a Delphi Study 1 April 1980Volume 92, Issue 4Page: 562-563KeywordsEpidemiology Issue Published: 1 April 1980 PDF DownloadLoading ...
1024-54 Low repeat revascularization rates following drug-eluting stent implantation in de novo bifurcation lesions
Authors: Angela Hoye, Pedro A Lemos, Kengo Tanabe, Chourmouzios Arampatzis, Francesco Saia, Jiro Aoki, Muzaffer Degertekin, Andrew T.L Ong, Carlos van Mieghem, Sjoerd H Hofma, Georgios Sianos, Pieter C Smits, Willem van der Giessen, Eugene McFadden, Pim de Feyter, Patrick W Serruys
Journal: Journal of the American College of Cardiology (2004)
NORMAL CEREBROSPINAL FLUID DYNAMICS IN SPINAL CORD TUMOR SUSPECTS
Authors: JAMES L. POPPEN
Journal: JAMA (1934)

As the result of the efforts of Ayer<sup>1</sup>and Stookey,<sup>2</sup>the technic of eliciting abnormal changes in cerebrospinal fluid dynamics has become so refined that an obstruction in the spinal canal can be detected earlier in the disease than formerly. Cases have been reported, however, in which tumors were found that appeared large enough to block the entire canal but normal dynamics were shown by lumbar puncture. The question therefore arises, What size must a tumor be in relation to the spinal canal before there are any demonstrable changes in dynamics? Patients have frequently been known to suffer from pains for years which were thought due to rheumatism, sciatica, sacro-iliac disease or neuritis, before any objective symptoms were detected to suspect an intraspinal lesion. In such instances a problem as to the value of a lumbar puncture always arises, especially when the tumor does not obliterate the subarachnoid
Activation of platelet function in Fabry's disease
Authors: Takashi Igarashi, Hitoshi Sakuraba, Yoshiyuki Suzuki
Journal: American Journal of Hematology (1986)

Abstract Increased platelet aggregation and high plasma concentration of β‐thromboglobulin were observed in hemizygotes and heterozygotes of Fabry's disease. Carbamazepine and phenytoin administered for the treatment of pains in these patients showed no significant effect on platelet aggregation. No activation of platelets was observed after the addition of ceramide trihexoside, the storage lipid of this disease. Mitral valve prolapse was found in eight of 12 patients. Although the pathogenesis of platelet activation and mitral valve prolapse are not known, the platelet activation could be an early indicator and an accelerating factor of thromboembolic vascular change in this disease.
Dosage of Aminopterin
Authors: Eugene H. Swanzey
Journal: JAMA (1962)

<h3>To the Editor:—</h3> It was called to our attention (<i>JAMA</i><b>178</b>:46 [Oct. 21] 1961) that a misstatement had been made in a correction. In this notation Dr. Kushner commented on an article, Treatment of Reticulosis (<i>JAMA</i><b>175</b>:84 [Jan. 14] 1961), wherein the dosage of aminopterin was incorrectly stated. We concur that this dosage is incorrect. However, Dr. Kushner is in error in stating that aminopterin has been taken off the market. Aminopterin is available for the treatment of acute leukemia in children, and we have no present intention of removing it from the market. Dr. Kushner, however, commits a misstatement which has caused considerable confusion in that he states "It so happens that amethopterin...." Dr. Kushner is referring to methotrexate. Methotrexate was formally known as amethopterin, but this name was discontinued because it is readily confused with aminopterin. Methotrexate has one, and only one, name. Methrotrexate is the
Scintigraphy in Pediatric Urinary Tract Lymphoma
Authors: JOHN R. STY, DONALD P. BABBIT, KUMUDCHANDRA J. SHETH
Journal: Clinical Nuclear Medicine (1978)

Excretory urography is the standard examination used to evaluate structural abnormalities of the urinary tract in children. However, renal imaging offers advantages over excretory urography in the functional evaluation and in certain morphologic patterns of lymphoma.
The Surgical Treatment of Migraine
Authors: W. Whitehead
Journal: BMJ (1901)

During the last five and twenty years I have never failed to treat successfully the most inveterate and severe cases of migraine by the introduction of an ordinary tape seton through the skin at the back of the neck.

My last case, and the one which has induced me to make this communication, is that of a lady who has never for the last six years escaped many weeks without “a brain storm” of such severity that …
Sublingual grass allergen tablet immunotherapy provides sustained clinical benefit with progressive immunologic changes over 2 years
Authors: Ronald Dahl, Alexander Kapp, Giselda Colombo, Jan G.R. de Monchy, Sabina Rak, Waltraud Emminger, Bente Riis, Pernille M. Grønager, Stephen R. Durham
Journal: Journal of Allergy and Clinical Immunology (2008)
Predicting Early Mortality After Acute Variceal Hemorrhage Based on Classification and Regression Tree Analysis
Authors: Salvador Augustin, Laura Muntaner, José T. Altamirano, Antonio González, Esteban Saperas, Joan Dot, Monder Abu–Suboh, Josep R. Armengol, Joan R. Malagelada, Rafael Esteban, Jaime Guardia, Joan Genescà
Journal: Clinical Gastroenterology and Hepatology (2009)