Detection of Deficient Erythrocyte Regeneration of Reduced Triphosphopyridine Nucleotide from Glucose-6-Phosphate
Authors: A. P. KRAUS
Journal: Annals of Internal Medicine (1962)

Article1 May 1962Detection of Deficient Erythrocyte Regeneration of Reduced Triphosphopyridine Nucleotide from Glucose-6-PhosphateEvaluation of a Rapid Screening TestA. P. KRAUS, M.D., F.A.C.P., C. L. NEELY, M.D., F. T. CAREY, M.D., L. M. KRAUS, PH.D.A. P. KRAUS, M.D., F.A.C.P.Search for more papers by this author, C. L. NEELY, M.D.Search for more papers by this author, F. T. CAREY, M.D.Search for more papers by this author, L. M. KRAUS, PH.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-56-5-765 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe treatment of malaria by the 8-aminoquinoline compounds was accompanied by a small incidence of hemolytic anemia (1, 2) particularly in the pigmented races. Dernet al. (3), in 1954, utilizing chromium-51 tagged erythrocytes demonstrated the abnormality to be an intracorpuscular defect. Fegler (4) noted that erythrocytes did not hemolyzein vitrountil the reduced glutathione (GSH) fell below 40 mg per 100 ml red blood cells. Benesch and Benesch (5) demonstrated that blockage of erythrocyte sulfhydryl groups by arsenite resulted in hemolysis. Since glutathione constitutes the principal source of sulfhydryl groups in the erythrocyte, this finding suggested that it...References1. MANSON-BAHR P: The action of plasmochin on malaria. Proc. Roy. Soc. Med. 20: 919, 1927. CrossrefMedlineGoogle Scholar2. CORDES W: Zwischenfälle bei der Plasmochinbehandlung. Arch. Schiffs Tropen. Hyg. 32: 143, 1928. Google Scholar3. DERNWEINSTEINLE ROYTALMAGEALVING RJIMGVDWAS: The hemolytic effect of primaquine. I. The location of the drug-induced hemolytic defect in primaquine-sensitive individuals. J. Lab. Clin. Med. 43: 303, 1954. MedlineGoogle Scholar4. FEGLER G: Relation between reduced glutathione content and spontaneous hemolysis in shed blood. Nature 170: 624, 1952. CrossrefMedlineGoogle Scholar5. BENESCHBENESCH RER: Relation between erythrocyte integrity and sulfhydryl groups. Arch. Biochem. 48: 38, 1954. CrossrefMedlineGoogle Scholar6. BEUTLERDERNALVING ERJAS: The hemolytic effect of primaquine. VI. An in vitro test for sensitivity of erythrocytes to primaquine. J. Lab. Clin. Med. 45: 40, 1955. MedlineGoogle Scholar7. CARSONFLANNAGANICKESALVING PECLCEAS: Enzymatic deficiency in primaquine-sensitive erythrocytes. Science 124: 484, 1956. CrossrefMedlineGoogle Scholar8. STANBURYWYNGAARDENFREDRICKSON JBJBDS: The Metabolic Basis of Inherited Disease, McGraw-Hill Book Company, Inc., New York, 1960, chap. 32. Google Scholar9. BEUTLER E: The glutathione instability of drug sensitive red cells. A new method for the in vitro detection of drug sensitivity. J. Lab. Clin. Med. 49: 84, 1957. MedlineGoogle Scholar10. ZINKHAMLENHARDCHILDS WHREB: A deficiency of glucose-6-phosphate dehydrogenase activity in erythrocytes from patients with favism. Bull. Johns Hopkins Hosp. 102: 169, 1958. MedlineGoogle Scholar11. MOTULSKYCAMPBELL AGJM: A screening test for glucose-6-phosphate dehydrogenase deficiency of the red cell suitable for genetic surveys. Possible relationship of the enzyme defect to malaria. To be published. Google Scholar12. MOTULSKYPAULDURRUM AGMHEL: Paper electrophoresis of abnormal hemoglobins and its clinical applications. Blood 9: 897, 1954. CrossrefMedlineGoogle Scholar13. KOHN J: Personal communication. Google Scholar14. A Textbook of Clinical Pathology, 6th ed., ed. by Miller, S. E., The Williams & Wilkins Co., Baltimore, chaps. I and II. Google Scholar15. WOODWARD GE: Glyoxalase. III. Glyoxalase as a reagent for the quantitative micro estimation of glutathione. J. Biol. Chem. 109: 1, 1935. CrossrefGoogle Scholar16. HSIA DY: Inborn Errors of Metabolism, The Year Book Publishers, Inc., Chicago, 1959, p. 331. Google Scholar17. NEELYKRAUS CLAP: The accidental transfusion of TPNH deficient erythrocytes into hospitalized patients. To be published. Google Scholar18. NAYLORROSENTHALGROSSMANSCHULMANHSIA JIAIDY: Glucose-6-phosphate dehydrogenase activity in patients with various abnormal hemoglobins. J. Dis. Child. 98: 622, 1959. Google Scholar19. CHILDSZINKHAM BW: A genetic study of a defect in glutathione metabolism of the erythrocyte. Bull. Johns Hopkins Hosp. 102: 21, 1958. MedlineGoogle Scholar20. RAMOTASHKENAZIRIMONADAMSHEBA BIAAC: Activation of glucose-6-phosphate dehydrogenase of enzyme-deficient subjects. II. Properties of the activator and the activator reaction. J. Clin. Invest. 40: 611, 1961. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: A. P. KRAUS, M.D., F.A.C.P.; C. L. NEELY, M.D.; F. T. CAREY, M.D.; L. M. KRAUS, PH.D.Affiliations: Memphis, TennesseeFrom the Department of Medical Laboratories and Division of Chemistry, University of Tennessee Medical Units, and City of Memphis Hospitals, Memphis, Tennessee.This investigation was supported by Public Health Service Research Grant A3803 from the Institute of Arthritis and Metabolic Diseases, and a research grant from American Lebanese Syrian Aslociated Charities-St. Jude.Requests for reprints should be addressed to A. P. Kraus, M. D., The University of Tennessee College of Medicine, Department of Medical Laboratories, 42 North Dunlap Street, Memphis 3, Tennessee. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byEnzymatic Methods Used for DiagnosisGlucose-6-Phosphate Dehydrogenase DeficiencyClinical and Hematological Studies in a Family with Hemoglobin VancouverCongenital hemolytic anemias and red cell enzyme deficienciesPrevalence of anemia among preadolescent and young adolescent urban black americansG-6-PD Deficiency Gene Dynamics in a Brazilian PopulationAn evaluation of screening procedures for red cell glucose-6-phosphate dehydrogenase deficiency in the newborn infantA Simple Visual Screening Test for Glucose-6-Phosphate Dehydrogenase Deficiency Employing Ascorbate and CyanideMedical genetics 1962 1 May 1962Volume 56, Issue 5_Part_1Page: 765-773KeywordsGlutathioneHealth services researchHemoglobinHemolytic anemiaMalariaMetabolic disordersRacial and ethnic issuesRed blood cellsRegenerative medicineResearch grants ePublished: 1 December 2008 Issue Published: 1 May 1962 PDF downloadLoading ...