tbuckley/cabot-search huggingface dataset:
Assay for Antibodies to Double-Stranded DNA
Authors: CHARLES R. STEINMAN
Journal: Annals of Internal Medicine (1979)

Letters and Corrections1 January 1979Assay for Antibodies to Double-Stranded DNACHARLES R. STEINMAN, M.D.CHARLES R. STEINMAN, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-90-1-128 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: I read with interest the report of Chubick and associates (1) comparing the crithidial immunofluorescence (CL-IF) method for detecting antibodies to double-stranded DNA (dsDNA) with selected alternate assays. As is increasingly recognized, at least two sorts of information about the DNA antigen used in such assays are necessary for adequate evaluation of data and for duplication of results. First is careful definition of the antigenic sites present (particularly with regard to possible contamination, chiefly with single-stranded DNA [ssDNA] and perhaps nucleoprotein) as well as of the DNA size. Although the authors say they recognize the importance of...References1. CHUBICKSONTHEIMERGILLIAMZIFF ARJM: An appraisal of tests for native DNA antibodies in connective tissue diseases. Clinical usefulness of Crithidia luciliae assay. Ann Intern Med 89:186-192, 1978 LinkGoogle Scholar2. STEINMANDEESOMCHOKSPIERA CUH: Detection of anti-DNA antibody using synthetic antigens. Characterization and clinical significance of binding of poly (deoxyadenylate-deoxythymidylate) by serum. J Clin Invest 57:1330-1341, 1976 CrossrefMedlineGoogle Scholar3. AARDENLAKMAKERFELTKAMP LFT: Immunology of DNA II. The effect of size and structure of the antigen on the Farr assay. J Immunol Methods 10:39-48, 1976. CrossrefMedlineGoogle Scholar1. STEINMANDEESOMCHOKSPIERA CUH: Detection of anti-DNA antibody using synthetic antigens. Characterization and clinical significance of binding of poly (deoxyadenylate-deoxythymidylate) by serum. J Clin Invest 57:1330-1341, 1976 CrossrefMedlineGoogle Scholar2. AARDENLAKMAKERDEGROOTSUAAKFELTKAMP LFEAT: Detection of antibodies to DNA by radioimmunoassay and immunofluorescence. Scand J Rheumatol 11(Suppl):12-19, 1975 CrossrefGoogle Scholar3. LAURENVAN ASSELSTEINERT MSM: Kinetoplast DNA. A unique macromolecular structure of considerable size and mechanical resistance. Biochem Biophys Res Comm 43:278-284, 1971 CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Mount Sinai School of Medicine of The City University of New York New York, NY 10029 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byThe role of antibodies directed against doublestranded DNA in the manifestations of systemic lupus erythematosus in childhood 1 January 1979Volume 90, Issue 1Page: 128-129KeywordsAntibodiesAntigensDNAImmunofluorescence Issue Published: 1 January 1979 PDF downloadLoading ...
COMBINED DRUG THERAPY COMPARED WITH ELECTRIC SHOCK IN PSYCHOTIC DEPRESSIONS
Authors: F. A. LINGL
Journal: American Journal of Psychiatry (1964)

One hundred patients hospitalized for psychotic types of depression were divided into two comparable groups. Half of the patients received 9 EST over a 3-week period. The other half received tranylcypromine and trifluoperazine b.i.d. The clinical symptoms were rated quantitatively prior to treatment and 4 weeks later. The response time to drugs was shorter, averaging 6.6 days versus 9.2 days in the EST group. The scores of the repeat evaluations and the average length of stay in the hospital were almost equal in both groups. A larger number of patients in the drug group engaged in activities spontaneously. No serious side effects were encountered on drugs and acceptance by the patients was much better than that of EST.
Reflections on the Doctor's Anguish
Authors: Dorothy R. Stewart
Journal: Annals of Internal Medicine (1993)

Letters1 January 1993Reflections on the Doctor's AnguishJoan Weinryb, MDJoan Weinryb, MDTemple University; Albert Einstein Medical Center; Philadelphia, PA 19141Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-118-1-199301010-00024 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:I read with interest the article by Edwards and Stolle [1] and felt great sympathy for the physicians. To usher people comfortably out of life is an important part of the physician's job. This ideal is often agreed to in principle but is often not fulfilled in practice.It appears that, after careful planning, the specialist and the attending physician delegated this delicate task to an intern. There is no one to whom such a responsibility should be shifted, especially an unattended trainee.The authors appeared to require the concurrence of all family members in the decision ...REFERENCE1. Edwards MJ, Tolle SW. Disconnecting a ventilator at the request of a patient who knows he will die: the doctor's anguish. Ann Intern Med. 1992; 117:254-7. Google Scholar Author, Article, and Disclosure InformationAffiliations: Temple University; Albert Einstein Medical Center; Philadelphia, PA 19141 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 January 1993Volume 118, Issue 1Page: 78-80KeywordsGeriatricsPatient advocacyVentilators Issue Published: 1 January 1993 CopyrightCopyright © 1993 by American College of Physicians. All Rights Reserved.PDF DownloadLoading ...
Factor Analysis of the Toronto Alexithymia Scale: Elucidation of a Polythetic Construct
Authors: Lee S. Mann, Thomas N. Wise, Laurel Shay
Journal: Psychotherapy and Psychosomatics (1992)

The Toronto Alexithymia Scale (TAS) offers a reliable method to measure alexithymia, a personality construct describing individuals endorsing the inability to identify and report emotions, processing a minimal fantasy life, utilizing an analytic cognitive style, and tending to somatize. Utilizing four cohorts of both patients and controls, a three-factor solution emerged from a principal-components analysis utilizing a varimax rotation. The factors of inability to identify and report feelings, an analytic cognitive style, and paucity of fantasy life were independent and internally reliable. These data are reviewed in the context of previous research and offer three subscales for future investigations utilizing the Toronto Alexithymia Scale.
Observations on an Alkali Resistant Thermostable Hemopoietic Principle in Liver.
Authors: C. M. Leevy
Journal: Annals of Internal Medicine (1962)

s1 April 1962Observations on an Alkali Resistant Thermostable Hemopoietic Principle in Liver.C. M. Leevy, M.D., F.A.C.P., H. Baker, Ph.D., O. Frank, Ph.D., H. Ziffner, M.D.C. M. Leevy, M.D., F.A.C.P.Search for more papers by this author, H. Baker, Ph.D.Search for more papers by this author, O. Frank, Ph.D.Search for more papers by this author, H. Ziffner, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-56-4-686_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptPrevious studies in our laboratory showed flushing doses of cyanocobalamine displaced hepatic B12 and an alkaline resistant thermostable substance (Factor X), both of which supported growth of microorganisms requiring B12. The present communication reports results of further investigations on the occurrence, nature, and clinical effects of Factor X.Hepatic venous serum exhibited no B12activity following alkaline hydrolysis, but despite such treatment, serum obtained after administering 100 micrograms of B12permitted growth ofLactobacillus leichmannii, Escherichia coli 113-3, Euglena gracilis, andOchromonas malhamensis. Maximum displacement of Factor X occurred at 30 minutes; its serum level rapidly declined during the succeeding... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Jersey City, N. J. (CI) PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 April 1962Volume 56, Issue 4Page: 686-686KeywordsEscherichia coliLactobacillusLiverResearch laboratories Issue Published: 1 April 1962 PDF downloadLoading ...
Treating the Enemy
Authors: Dean L. Winslow
Journal: Annals of Internal Medicine (2007)

On Being a Doctor21 August 2007Treating the EnemyDean L. Winslow, MDDean L. Winslow, MDFrom Stanford University School of Medicine and Santa Clara Valley Medical Center, San Jose, California.Author, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-147-4-200708210-00013 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail The telephone rings at the front desk at our U.S. Air Force EMEDS hospital in Baghdad and is promptly answered by the Master Sergeant enlisted medic. "Sir, they need you over at the morgue—2 casualties—U.S." It is early spring in Baghdad and a light rain is falling. I grab a radio, pick up the keys to one of our assigned vehicles and drive the 500 meters to the ramshackle building near the flight line. Coalition casualties are brought here in heavy black body bags to be processed; after that, they are transferred to sturdy metal caskets covered by an American ... Author, Article, and Disclosure InformationAffiliations: From Stanford University School of Medicine and Santa Clara Valley Medical Center, San Jose, California.Disclaimer: The views expressed in this article represent those of the author alone and are not official statements of the Air National Guard, the U.S. Air Force, or the U.S. Department of Defense.Corresponding Author: Dean L. Winslow, MD, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, 300 Pasteur Drive, Grant Building, Room S-169, Stanford, CA 94305-5107; e-mail, [email protected]edu. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byProviders' roles in enhancing patients' adherence to pain self managementMedical Care for Terrorists—To Treat or Not to Treat?Increased Mortality Rates of Young Children With Traumatic Injuries at a US Army Combat Support Hospital in Baghdad, Iraq, 2004 21 August 2007Volume 147, Issue 4Page: 278-279KeywordsBloodElectrocardiographyEmergency departmentHeart rateInfectious diseasesIschemiaOxygenSurgeonsTroponinUltrasound imaging ePublished: 21 August 2007 Issue Published: 21 August 2007 Copyright & PermissionsCopyright © 2007 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Serum lipid elevation during isotretinoin therapy for acne in the west of Scotland
Authors: C. S. Munro, J. R. Marsden
Journal: British Journal of Dermatology (1991)

Journal Article Serum lipid elevation during isotretinoin therapy for acne in the west of Scotland Get access C. S. Munro, C. S. Munro Department of Dermatology, Royal Victoria Infirmary, Newcastle upon Tyne NE1 4LP, UK. Search for other works by this author on: Oxford Academic Google Scholar J. R. Marsden J. R. Marsden Department of Dermatology, The General Hospital, Steelhouse Lane, Birmingham B4 6NH, U.K. Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 124, Issue 3, 1 March 1991, Page 301, https://doi.org/10.1111/j.1365-2133.1991.tb00583.x Published: 01 March 1991
High-Dose Vitamin E Does Not Decrease the Rate of Chronic Hemolysis in Glucose-6-Phosphate Dehydrogenase Deficiency
Authors: Gerhard J. Johnson, Govind T. Vatassery, Barbara Finkel, David W. Allen
Journal: New England Journal of Medicine (1983)

Several observations made during in vitro and in vivo studies of animal and human erythrocytes have indicated that vitamin E may protect erythrocytes from oxidant-induced damage.1 Previous reports from our laboratory have presented evidence indicating that chronic hemolysis secondary to glucose-6-phosphate dehydrogenase deficiency is attributable to oxidant-induced erythrocyte-membrane injury.2 3 4 Since vitamin E may function as an antioxidant in erythrocytes, its use in patients with glucose-6-phosphate dehydrogenase deficiency accompanied by chronic hemolytic disease has been investigated.1 , 5 , 6 Two of these studies indicated that high-dose vitamin E reduced the rate of hemolysis in patients with glucose-6-phosphate dehydrogenase deficiency,1 , 5 whereas no change was observed . . .
Mutations in HFE, the Hemochromatosis Candidate Gene, in Patients With NIDDM
Authors: Danièle Dubois-Laforgue, Sophie Caillat-Zucman, Idriss Djilali-Saiah, Etienne Larger, Anne Mercadier, Christian Boitard, Jean-François Bach, José Timsit
Journal: Diabetes Care (1998)
RECOMBINANT HUMAN GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR PLUS THE BEAM REGIMEN INSTEAD OF AUTOLOGOUS BONE MARROW TRANSPLANTATION
Authors: L. Fouillard, N.C. Gorin, J.Ph. Laporte, L. Douay, F. Isnard, A. Najman
Journal: The Lancet (1989)
Changes in gene expression following short coronary occlusions studied in porcine hearts with run-on assays
Authors: R. Knoll, M. Arras, R. Zimmermann, J. Schaper, W. Schaper
Journal: Cardiovascular Research (1994)

Journal Article Changes in gene expression following short coronary occlusions studied in porcine hearts with run-on assays Get access Ralph Knöll, Ralph Knöll Max-Planck-Institute, Department of Experimental Cardiology, Benekestrasse 2, D-61231 Bad Nauheim, Germany: R Knöll, M Arras, R Zimmermann, J Schaper, W Schaper. Correspondence to Professor W Schaper. Search for other works by this author on: Oxford Academic PubMed Google Scholar Margarete Arras, Margarete Arras Max-Planck-Institute, Department of Experimental Cardiology, Benekestrasse 2, D-61231 Bad Nauheim, Germany: R Knöll, M Arras, R Zimmermann, J Schaper, W Schaper. Search for other works by this author on: Oxford Academic PubMed Google Scholar René Zimmermann, René Zimmermann Max-Planck-Institute, Department of Experimental Cardiology, Benekestrasse 2, D-61231 Bad Nauheim, Germany: R Knöll, M Arras, R Zimmermann, J Schaper, W Schaper. Search for other works by this author on: Oxford Academic PubMed Google Scholar Jutta Schaper, Jutta Schaper Max-Planck-Institute, Department of Experimental Cardiology, Benekestrasse 2, D-61231 Bad Nauheim, Germany: R Knöll, M Arras, R Zimmermann, J Schaper, W Schaper. Search for other works by this author on: Oxford Academic PubMed Google Scholar Wolfgang Schaper Wolfgang Schaper Max-Planck-Institute, Department of Experimental Cardiology, Benekestrasse 2, D-61231 Bad Nauheim, Germany: R Knöll, M Arras, R Zimmermann, J Schaper, W Schaper. Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 28, Issue 7, July 1994, Pages 1062–1069, https://doi.org/10.1093/cvr/28.7.1062 Published: 01 July 1994 Article history Received: 16 November 1993 Accepted: 24 February 1994 Published: 01 July 1994
Partial purification of a high molecular weight renin from hog kidney.
Authors: M Levine, K E Lentz, J R Kahn, F E Dorer, L T Skeggs
Journal: Circulation Research (1976)

Previous investigators have reported finding a high molecular weight (HMW) renin in various species which is activated by acidification. Two laboratories have reported that a decrease in molecular weight of the renin accompanies the acidification step. This report describes the partial purification of an HMW renin from hog kidney extracts which had previously been acidified to pH 2.5. The HMW renin, with a molecular weight of 57,000-59,000, constitutes only a small, relatively constant fraction of the total renin isolated. Omission of the acidification step or initial acidification to pH 1.6 does not change the amount of HMW renin significantly. The HMW renin attacks the protein substrate to produce angiotensin at about one-fourth the rate expected, based upon the rate at which it cleaves the tetradecapeptide substrate or a model nonapeptide substrate. It is inactivated by antiserum to hog kidney renin prepared in dogs. It is less stable than hog renin when stored at 0 degrees C. The HMW renin has a pH optimum between 6.5 and 7.0. It is not activated by acidification to pH 3, nor by tryptic or peptic digestion. We have been unable to activate HMW renin or to change its molecular weight by procedures reported by other investigators and therefore conclude that the HMW renin differs from material previously reported and may be an isoenzyme of renin.
Expression of Matrix Metalloproteinase (MMP)-2 and MMP-9 in Breast Cancer with a Special Reference to Activator Protein-2, HER2, and Prognosis
Authors: Johanna M. Pellikainen, Kirsi M. Ropponen, Vesa V. Kataja, Jari K. Kellokoski, Matti J. Eskelinen, Veli-Matti Kosma
Journal: Clinical Cancer Research (2004)

Abstract Purpose: In the present study, we investigated the expression and prognostic value of matrix metalloproteinase (MMP)-2 and MMP-9 in breast cancer as well as their relation to transcription factor activator protein (AP)-2 and HER2 oncogene. The role of invasion and metastasis-promoting MMPs and their potential regulators, AP-2 and HER2, is currently still unclear in breast cancer. Experimental Design: MMP-2 and MMP-9 expressions were analyzed immunohistochemically in a large prospective series of 421 breast cancer patients diagnosed and treated between 1990 and 1995 at Kuopio University Hospital (Kuopio, Finland). The relation of MMP-2 and MMP-9 expressions to AP-2, HER2, clinicopathological data, and survival was investigated. Results: Both MMP-2 and MMP-9 were expressed in the cytoplasm of malignant and stromal cells. High expression of MMPs in carcinoma cells was related to small tumors (T1, stage I), whereas positive stromal expression of MMPs was associated with aggressive factors. High expression of MMP-2 and MMP-9 in carcinoma cells, but not in stromal cells, was related to high AP-2 expression. Positive stromal MMP-2 expression was associated with HER2 overexpression in the whole patient group and in the node-negative patient subgroup. Positive stromal MMP-9 expression was related to HER2 overexpression in estrogen receptor (ER)-positive disease. In the univariate survival analysis, positive stromal MMP-9 predicted shorter recurrence-free survival (RFS; P = 0.0389) and breast cancer-related survival (BCRS; P = 0.0081) in ER+ disease, especially in the subgroup of ER+ tumors of ≤2 cm in diameter (T1; P = 0.0031 for RFS, and P = 0.0089 for BCRS). High MMP-9 expression in cancer cells predicted longer RFS (P = 0.0351) in the whole patient group. In the multivariate analysis of the whole patient group, the independent predictors of shorter RFS were reduced MMP-9 expression in carcinoma cells (P = 0.0248), HER2 overexpression (P = 0.0001), and advanced-stage disease (P = 0.0002). Shorter BCRS was predicted by advanced-stage disease (P &amp;lt; 0.0001). Conclusions: Expression of MMP-2 and MMP-9 in breast cancer seems to be partly related to expression of AP-2 and HER2. Positive stromal MMP-9 expression predicts poor survival in the hormone-responsive small tumors, whereas MMP-9 expression in carcinoma cells favors survival. Evaluation of MMP-9 expression seems to add valuable information on breast cancer prognosis.
Head and Neck Cancer Arising After Endoscopic Mucosal Resection for Squamous Cell Carcinoma of the Esophagus
Authors: Y. Shimizu, H. Tsukagoshi, M. Fujita, M. Hosokawa, A. Watanabe, S. Kawabori, M. Kato, T. Sugiyama, M. Asaka
Journal: Endoscopy (2003)

It is well known that patients with head and neck cancer often have synchronous or metachronous squamous cell carcinoma of the esophagus. However, the prevalence of subsequent head and neck cancer in patients with early-stage esophageal cancer is still unknown. The aims of this study were to analyze the frequency of metachronous head and neck cancer after endoscopic mucosal resection (EMR) for esophageal cancer and to investigate whether minute iodine-unstained areas, often associated with squamous cell carcinomas, would be an index for metachronous head and neck cancer.99 patients with esophageal squamous cell carcinoma who underwent EMR were studied. Based on the iodine-staining pattern at initial EMR, they were categorized into those with uniform (group U) and scattered (group S) types of background mucosa. Patients were monitored endoscopically and otolaryngologically (group U, median 46 months, range 12-83 months; group S, median 44 months, range 13-80 months).In total, 5/99 patients (5.1 %) were found to have metachronous head and neck cancer during the follow-up, including 4/20 patients (20 %) in group S. In three cases laryngeal or hypopharyngeal cancer was found by endoscopic examination. The cumulative proportion of metachronous head and neck cancer-free subjects was significantly lower in group S than group U (P = 0.0007).Among patients who undergo EMR for esophageal carcinoma, those with scattered-type iodine staining of the background mucosa have an increased risk of metachronous head and neck cancer, and should therefore be closely observed. Careful endoscopic observation led to early detection of laryngeal and hypopharyngeal cancer.
Counting the cost of medical negligence
Authors: P. Fenn
Journal: BMJ (2002)

The financial cost of medical negligence is a topic that rarely recedes from the headlines. In part this is due to a perception that the money paid out to patients is a measure of the adverse health consequences of medical errors, but in part it is due to a concern over the impact such payments will have on healthcare providers themselves. Each million pounds paid in damages is a million pounds that otherwise could be spent on patient care. What is sometimes overlooked is that this financial impact on providers can fulfil a positive role—it gives a signal of where things are going wrong, and an incentive to put them right. For these reasons it is important for consistent data to be collected on the frequency and cost of medical incidents.

Given the interest generated by the topic, it is perhaps surprising that so little is known with confidence about the cash cost of clinical negligence to the English health service. In a recent article in this journal,1 my colleagues and I attempted to use information from hospitals in the Oxford region to extrapolate a national figure for cash paid out by the NHS in 1998-9. We arrived at a figure in the range of £48m to £130m, which we now believe may be an underestimate owing to the small number of very large claims in our sample. The National Audit Office, in its recent review of claims …