tbuckley/cabot-search huggingface dataset:
CELL SURFACE ANTIGENS OF A MOUSE TESTICULAR TERATOMA
Authors: Linda R. Gooding, Michael Edidin
Journal: The Journal of Experimental Medicine (1974)

Rabbit antisera to a mouse testicular teratoma, absorbed with normal mouse tissues, react by immunofluorescence with plasma membrane antigens of a variety of transplantable mouse tumor cells and transformed fibroblast cell lines including Clone 1D, SV-40-3T3, and 3T12. Trypsin treatment of cells of "normal" lines, 3T3 and FR-SV-3T3, uncovers reactivity on these as well. Early passage mouse embryo fibroblast cell cultures do not react even after trypsinization. By cross-absorbtion studies, the anti-teratoma serum appears to react with an antigen common to most tumor cells investigated thus far. When this antigen on Clone 1D cells is "capped," H-2 antigens collect with the teratoma antigens in the cap indicating a physical association between the molecules. Molecules specified by both the H-2D and H-2K regions are bound to the teratoma antigens in the Clone 1D plasma membrane. This antigen is also found in soluble tumor cell fractions where it is believed to be free of H-2. A second cell surface antigen defined by anti-teratoma serum is expressed only by hepatoma and teratoma itself. This second antigen is apparently a secretory product of teratoma cells. A third surface antigen defined by anti-teratoma serum appears to be specific for the teratoma.
EXTRACTION OF PEMPHIGUS ANTIBODIES FROM A LYMPHOID NEOPLASM AND ITS POSSIBLE RELATIONSHIP TO PEMPHIGUS VULGARIS
Authors: N. K. SAIKIA
Journal: British Journal of Dermatology (1972)

Summary.— A patient is described who had a malignant lymphoid neoplasm and later developed pemphigus vulgaris. Tumour homogenate from the neoplasm contained a high titre of pemphigus antibodies.
OPTIMIZATION OF DEMETHYLATING THERAPY FOR IDH1 MUTANT GLIOMAS
Authors: G. J. Riggins, A. Borodovsky
Journal: Neuro-Oncology (2014)

BACKGROUND: Mutations in Isocitrate Dehydrogenase 1 (IDH1) are found in the majority of grade II and III gliomas and the subsequent progressive GBMs. The mutant IDH1 enzyme produces 2-hydroxyglutarate (2-HG), an “oncometabolite” that inhibits α-ketogluterate dependent histone and DNA demethylases resulting in characteristic hypermethylation of genomic DNA and suppression of cellular differentiation as a key mechanism of tumor progression and maintenance. METHODS: To accurately test therapeutics for IDH1 mutant gliomas, we developed a patient derived IDH1 mutant anaplastic astrocytoma model which produces 2-HG and exhibits characteristic DNA hypermethylation. This IDH1 mutant glioma grows only as a xenograft and not in cell culture. We measured tumor growth using a flank model in 5-azacytidine treated vs. untreated animals, passaging both sets of tumors from animal to animal in order to increase the exposure time to the drug. Tumor size was measured for treated vs. untreated mice and tumors were analyzed for gene expression, differentiation markers and promoter DNA methylation. Methylation status was by pyrosequencing analysis of bisulfate-converted genomic DNA at five genetic loci, with subsequent studies assessing global gene promoter methylation status. RESULTS: Long term administration of 5-azacytidine resulted in reduction of DNA methylation of promoter loci, induction of glial differentiation, reduction of cell proliferation and significantly reduced tumor growth. Nearly complete tumor regression was observed by 14 weeks, resulting in a sustainable response and significant survival benefit. When therapy was discontinued, tumors did not show any signs of re-growth for at least an additional 8 weeks. Statistical analyses were performed using a student t-test for comparisons between the treatment groups. Following one cycle of 5-azacytidine treatment, GFAP expression was strongly increased and protein levels were maintained throughout subsequent cycles. 5-azacytidine treatment significantly reduced the fraction of Ki-67 positive cells in a time dependent manner and loss of DNMT1 expression in vivo following one treatment cycle. CONCLUSIONS: Using this genetically and molecularly accurate model we have demonstrated the preclinical efficacy and mechanism of action of the FDA approved demethylating drug 5-azacytidine in vivo. The goal of this work is to optimize treatment efficacy and to precisely define the molecular alterations responsible for successful therapy. Long term administration of 5-azacytidine resulted in tumor regression acting through the expected mechanism of reversal of DNA methylation, and promoting a more normal cellular differentiation process. Optimization of demethylating therapy shows promise for IDH1 mutant gliomas. SECONDARY CATEGORY: Tumor Biology.
HL-A antigens and vitiligo*
Authors: G. RETORNAZ, H. BETUEL, J.P. ORTONNE, J. THIVOLET
Journal: British Journal of Dermatology (1976)

HL-A antigens were found with similar frequency in ninety vitiligo patients and in 341 healthy controls. However there was a significant correlation between HL-A13 and vitiligo with antithyroid antibodies. This supports the concept that vitiligo is a syndrome rather than a disease.
CICATRIZATION OF WOUNDS
Authors: Alexis Carrel
Journal: The Journal of Experimental Medicine (1921)

As long as the wounds were protected by a connective tissue dressing against mechanical, chemical, and bacterial irritations, no evidence of cicatrization was found. The complete or partial failure of four experiments was due to the slipping of the inner dressing from the wound, mechanical irritation by the gauze, and infection. In the two experiments in which the connective tissue was maintained at the surface of the wound, there was no beginning of cicatrization, although 25 and 18 days respectively had elapsed since the operation, while in the control wound the duration of the latent period did not exceed 5 or 6 days. The experiments were interrupted after the second or third inspection, on account of the technical impossibility of again applying to the wounds a non-irritant dressing. It is probable that the wounds could have been kept for a much longer time in a condition of quiescence. While it is not known whether cicatrization could be prevented for an indefinite period, there is no doubt that the mechanism of regeneration is not set in motion at the usual time, when all external irritations are suppressed. It appears, therefore, that under ordinary conditions, cicatrization is not initiated by an internal factor. On the contrary, the application of turpentine, chick embryo pulp, and staphylococci decreased markedly the length of the latent period, which was often reduced to less than 2 days. This fact demonstrated the importance of external factors in the initiation of cicatrization. It seems that the mechanism of regeneration has become adapted to the ordinary conditions of life of the animals. A small wound will begin to cicatrize sooner if slightly infected, as practically always happens, than if it were thoroughly protected by a non-irritant dressing.
Anna Stephenie Wilson (nee Kilner)
Authors: M. Wilson, F. Coyne, P. Burrows
Journal: BMJ (2010)

Anna qualified in London in 1971 and passed her MRCGP in 1979. During 1976-8 she was company doctor in Kitwe, Zambia, and in 1980-1 she was student health physician at Universiti Malaya in Kuala Lumpur. After working in south east London and Wolverhampton, she moved to Winchester and worked as a community child health physician. In 1991 she re-entered general practice at the Gratton Surgery in Sutton Scotney, a rural dispensing practice which provided medical services to the military, and she rapidly became a much-loved general practitioner.

She joined the board of the Wessex Faculty of the Royal College of General Practitioners in 1992 and became its chairman …
Guidelines of the French Society for Digestive Endoscopy (SFED) 
Authors: B. Napoléon, B. Boneu, L. Maillard, C.-M. Samama, J.-F. Schved, G. Gay, T. Ponchon, D. Sautereau, J.-M. Canard
Journal: Endoscopy (2006)

Managing patients who are taking Vitamin K Antagonists (VKA) or antiplatelet agents (APA) is a common problem before performing digestive endoscopy. Although risks concerning bleeding are the remit of both the gastroenterologist and anaesthetist, the potential risk of interruption of antithrombotic treatment for the patient should not be underestimated. Faced with the wide variety of situations, it is difficult to lay down rules which cover all circumstances and there are few reference guidelines [1] [2] [3]. Ideally, changes of treatment ought to be made in consultation with the doctor who initially prescribed the treatment with estimation of the risk/benefit ratio bearing in mind alterations to the antithrombotic treatment. This has to be tailored to individual patients on a case-by-case basis.This ideal situation is rare. Often the patient cannot remember the reference physician or the latter cannot be contacted. Other factors rendering such strategies difficult and complex include differences of opinion or lack of knowledge about the respective risks of stopping antithrombotic treatment or of not performing the endoscopy, lack of data in the literature, etc. However, in practice there are some data on which to base a decision. Three factors should be taken into account: the risk of haemorrhage from the procedure, the antithrombotic treatment used and the risk of thromboembolism when the treatment is withdrawn. In an attempt to reach the broadest possible consensus, the French Society of Digestive Endoscopy (SFED), the Study Group on Haemostasis and Thrombosis (GEHT) of the French Society of Haematology, the French Society of Cardiology (SFC) and the French Society of Anaesthesiology and Intensive Care Medicine (SFAR) have joined forces and put forward these recommendations. Of course, they are still only temporary guidelines and are likely to change over time as scientific knowledge accrues.
Naloxone and Blood Pressure
Authors: RICHARD STRAUSS
Journal: Annals of Internal Medicine (1983)

Letters and Corrections1 May 1983Naloxone and Blood PressureRICHARD STRAUSS, M.D.RICHARD STRAUSS, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-98-5-672_3 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: I found the article by Higgins and colleagues (1) interesting and agree that their preliminary data provide evidence for the possible clinical use of naloxone in shock states. However, there are two points with which I take issue.First, the authors point to a pulmonary capillary wedge pressure of 14 mm Hg as adequate evidence to exclude hypovolemia as a contributing factor to their patient's shock state without data on the amounts and results of their fluid challenges. It is difficult to know if this patient was euvolemic. Chronic and acute changes in ventricular compliance can manifest...References1. HIGGINSSIVAKO'NEILLGRAVESFOUTCH TEDJD. Reversal of hypotension by continuous naloxone infusion in a ventilator-dependent patient. Ann Intern Med. 1983;"98:47-8. LinkGoogle Scholar2. SIBBALDCALVINDRIEDGES WJA. Right and left ventricular preload, and diastolic ventricular compliance: implications for therapy in critically ill patients. In: SHOEMAKER WC, THOMPSON WL, eds. Critical Care State of the Art. Fullerton, California: Society of Critical Care Medicine; 1982:(F) 1-33. Google Scholar3. FLACKEFLACKEWILLIAMS JWG. Acute pulmonary edema following naloxone reversal of high-dose morphine anesthesia. Anesthesiology. 1977;47:376-8. CrossrefMedlineGoogle Scholar4. TANAKA G. Hypertensive reaction to naloxone. JAMA. 1974;228:25-6. CrossrefMedlineGoogle Scholar5. ESTILOCOTTRELL AJ. Naloxone, hypertension, and ruptured cerebral aneurysm. Anesthesiology. 1981;54:352. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByAdvances in Clinical Toxicology 1 May 1983Volume 98, Issue 5_Part_1Page: 672-673KeywordsBlood pressurePulmonary capillary wedgeShock Issue Published: 1 May 1983 PDF DownloadLoading ...
ELECTRO-THERMIC HEMOSTASIS IN ABDOMINAL AND PELVIC SURGERY.
Authors: ANDREW J. DOWNES
Journal: JAMA (1901)

With the development of aseptic surgery arose the necessity in hemostasis, not only of a certain method of occluding the vessels, but of doing it with an agent which would not add a complication to the case either immediately or remotely. No method of hemostasis generally used fulfills this definition. The use of the actual cautery leaves carbonized tissue, a slough and the danger of subsequent hemorrhage. The objections to silk ligatures, especially the size required for large blood vessels and pedicles in the pelvis, are only too well known. The introduction of sterile absorbable ligatures bid fair to solve the problem; yet we have lately the angiotribe, which is also uncertain, for Doyen and those following him use it now simply to compress a groove in which the animal ligature is applied without danger of its slipping. The angiotribe alone, without the addition of the ligature, is slow and
Long-term follow-up of patients after transcatheter direct current ablation of the atrioventricular junction
Authors: Mårten Rosenqvist, Michael A. Lee, Laurence Moulinier, Michael J. Springer, Joseph A. Abbott, Joan Wu, Jonathan J. Langberg, Jerry C. Griffin, Melvin M. Scheinman
Journal: Journal of the American College of Cardiology (1990)
T Lymphocyte Rosette Formation After Major Burns
Authors: Barbara A. Neilan
Journal: JAMA (1977)

The ability of lymphocytes to form rosettes with sheep erythrocytes was studied in the acute burn period to quantitate T lymphocytes. Seventeen adult patients with burns of greater than 15% body surface area were studied serially and compared to normal controls. The absolute number of total rosette-forming cells (RFC) averaged 1,229.1±92.8/cu mm (SEM) for controls. In patients, a conspicuous decrease in absolute number of total RFC occurred at days 3 to 5 postburn (578.0±144.2/cu mm) (<i>P</i>&lt;.001) and during days 6 to 10 (799.8±106.1/cu mm) (<i>P</i>&lt;.01). Similarly, the percentage total RFC in patients at days 6 to 10 (48.9%±3.3%) was significantly lower than controls (73.9%±0.7%) (<i>P</i>&lt;.01). The decrease in total RFC did not correlate with the total lymphocyte count or the cortisol level. These findings suggest that T lymphocytes are decreased or have altered rosette-forming ability in the early postburn period and are in accordance with other reports of impaired cellular immunity following burns. (<i>JAMA</i>238:493-496, 1977)
In vitro studies of poison oak immunity. I. In vitro reaction of human lymphocytes to urushiol.
Authors: V S Byers, W L Epstein, N Castagnoli, H Baer
Journal: Journal of Clinical Investigation (1979)

Poison oak, ivy, and sumac dermatitis is a T-cell-mediated reaction against urushiol, the oil found in the leaf of the plants. This hapten is extremely lipophilic and concentrates in cell membranes. A blastogenesis assay employing peripheral blood lymphocytes obtained from humans sensitized to urushiol is described. The reactivity appears 1--3 wk after exposure and persists from 6 wk to 2 mon. The dose-response range is narrow, with inhibition occurring at higher antigen concentrations. Urushiol introduced into the in vitro culture on autologous lymphocytes, erythrocytes and heterologous erythrocytes produces equal results as measured by the optimal urushiol dose, the intensity of reaction, and the frequency of positive reactors. This suggests that the urushiol is passed from introducer to some other presenter cell. Although the blastogenically reactive cell is a T cell, there is also a requirement for an accessory cell, found in the non-T-cell population, for reactivity. Evidence is presented that this cell is a macrophage.
hOCT1 transcript levels and single nucleotide polymorphisms as predictive factors for response to imatinib in chronic myeloid leukemia
Authors: A Bazeos, D Marin, A G Reid, G Gerrard, D Milojkovic, P C May, H de Lavallade, P Garland, K Rezvani, J F Apperley, J M Goldman, L Foroni, J S Khorashad
Journal: Leukemia (2010)
Prediction of cardiac events after uncomplicated myocardial infarction: a prospective study comparing predischarge exercise thallium-201 scintigraphy and coronary angiography.
Authors: R S Gibson, D D Watson, G B Craddock, R S Crampton, D L Kaiser, M J Denny, G A Beller
Journal: Circulation (1983)

The ability of predischarge quantitative exercise thallium-201 (201T1) scintigraphy to predict future cardiac events was evaluated prospectively in 140 consecutive patients with uncomplicated acute myocardial infarction; the results were compared with those of submaximal exercise treadmill testing and coronary angiography. High risk was assigned if scintigraphy detected 201T1 defects in more than one discrete vascular region, redistribution, or increased lung uptake, if exercise testing caused ST segment depression greater than or equal to 1 mm or angina or if angiography revealed multivessel disease. Low risk was designated if scintigraphy detected a single-region defect, no redistribution, or no increase in lung uptake, if exercise testing caused no ST segment depression or angina, or if angiography revealed single-vessel disease or no disease. By 15 +/- 12 months, 50 patients had experienced a cardiac event; seven died (five suddenly), nine suffered recurrent myocardial infarction, and 34 developed severe class III or IV angina pectoris. Compared with that of patients at low risk, the cumulative probability of a cardiac event was greater in high-risk patients identified by scintigraphy (p less than .001), exercise testing (p = .011), or angiography (p = .007). Scintigraphy predicted low-risk status better than exercise testing (p = .01) or angiography (p = .05). Each predicted mortality with equal accuracy. However, scintigraphy was more sensitive in detecting patients who experienced reinfarction or who developed class III or IV angina. When all 50 patients with events were combined, scintigraphy identified 47 high-risk patients (94%), whereas exercise-induced ST segment depression or angina detected only 28 (56%) (p less than .001). The presence of multivessel disease as assessed by angiography identified nine more patients with events than exercise testing (p = .06). However, the overall sensitivity of angiography was lower than that of scintigraphy (71% vs 94%; p less than .01) because three patients who experienced reinfarction and 10 who developed class III or IV angina had single-vessel disease. Importantly, 12 (92%) of these 13 patients with single-vessel disease who had an event exhibited redistribution on scintigraphy. These results indicate that (1) submaximal exercise 201T1 scintigraphy can distinguish high- and low-risk groups after uncomplicated acute myocardial infarction before hospital discharge; (2) 201T1 defects in more than one discrete vascular region, presence of delayed redistribution, or increased lung thallium uptake are more sensitive predictors of subsequent cardiac events than ST segment depression, angina, or extent of angiographic disease; and (3) low-risk patients are best identified by a single-region 201T1 defect without redistribution and no increased lung uptake.
Histopathologic comparison of culprit vs. non-culprit lesions in the same patient: Multivessel atherectomy tissue analysis in acute coronary syndromes
Authors: Samin K. Sharma, Saeed Siddiqui, Billie Fyfe, Ram Bongu, Srinivas Duvvuri, Jonathan D. Marmur, Thomas P. Cocke, John A. Ambrose
Journal: Journal of the American College of Cardiology (1996)
Composition of bronchopulmonary secretions from patients with bronchiectasis.
Authors: T D Brogan, B H Davies, H C Ryley, P J Ross, L Neale
Journal: Thorax (1980)

Pulmonary function tests were carried out on 17 patients with bronchiectasis and six indices were selected to grade severity. Average concentrations of nine plasma proteins were determined by quantitative immunoelectrophoresis in specimens of sputum and serum from each patients. Wide patient-to-patient variation in concentration was encountered which appeared to follow a continuous progression unrelated to clinical severity. Patients at the upper end of the scale appeared to be responding by exudation and a pulmonary hypersensitivity reaction may be occurring in their lungs. There was evidence of selective concentration in sputum of alpha 1-antichymotrypsin and IgA although IgA concentrations were lower than would be expected in patients with chronic bronchitis. It is suggested that sputum IgA levels could be explored as a diagnostic criterion in those patients who could equally be suffering from chronic bronchitis.
Endoscopic submucosal dissection (ESD) section: An overview of articles in this issue
Authors: T. Rösch
Journal: Endoscopy (2006)

This issue of ”Endoscopy” contains a series of articles highlighting different aspects of the technique of endoscopic submucosal dissection (ESD) in the treatment of selected gastric tumors (early gastric cancers and a minority of gastric adenomas). ESD was developed to enable operators to resect lesions en bloc, even the larger ones, in contrast to the conventional technique of endoscopic mucosal resection (EMR), which is often performed piecemeal, resulting in difficulties in making a histopathological assessment of the completeness of resection.
Activation of antigen-induced lymphocyte proliferation by interleukin-15 without the mitogenic effect of interleukin-2 that may induce human immunodeficiency virus-1 expression.
Authors: A H Patki, M E Quiñones-Mateu, D Dorazio, B Yen-Lieberman, W H Boom, E K Thomas, M M Lederman
Journal: Journal of Clinical Investigation (1996)

The newly identified cytokine, IL-15 enhanced antigen-induced proliferation of PBMC obtained from HIV-1-seropositive subjects. When compared to IL-2 which enhanced both spontaneous and antigen-induced lymphocyte proliferative responses, IL-15 rarely increased spontaneous lymphocyte proliferation. Additionally, in cultures of lymphocytes obtained from 15 HIV-1-infected patients with < 300 circulating CD4- lymphocytes/microliter IL-15 induced significant HIV-1 expression (46, 21, and 71 pg/ml) in only 3 of 15 experiments and IL-2 induced significant HIV-1 expression (range 16- > 5000 pg/ml) in 11 of 15 experiments (P < 0.01, Fischer's exact test). Simultaneous assays of cytokine-induced spontaneous lymphocyte proliferation and HIV-1 expression revealed similar dose-response relationships for induction of HIV-1 and lymphocyte proliferation by IL-2. Thus, IL-15 helps to correct the impaired proliferative response of CD4+ lymphocytes from HIV-1-infected persons without the mitogenic effect of IL-2 that also may induce HIV-1 expression.