tbuckley/cabot-search huggingface dataset:
Transgenic Enrichment of Cardiomyocytes From Human Embryonic Stem Cells
Authors: David Anderson, Tim Self, Ian R Mellor, Gareth Goh, Stephen J Hill, Chris Denning
Journal: Molecular Therapy (2007)

To realize the full scientific and clinical potential of human embryonic stem cell (hESC)-cardiomyocytes, strategies to overcome the high degree of heterogeneity of differentiated populations are required. Here we demonstrate the utility of two transgenic approaches in enrichment of cardiomyocytes derived from HUES-7 cells: (i) negative selection of proliferating cells with the herpes simplex virus thymidine kinase/ganciclovir (HSVtk/GCV) suicide gene system; and (ii) positive selection of cardiomyocytes expressing a bicistronic reporter [green fluorescent protein (GFP)-internal ribosome entry site (IRES)-puromycin-N-acetyltransferase (PAC)] from the human alphamyosin heavy chain promoter. Parental and transgenic HUES-7 cells were similar with regard to morphology, pluripotency marker expression, differentiation, and cardiomyocyte electrophysiology. Whereas immunostaining of dissociated cardiomyocyte preparations expressing HSVtk or PAC contained <7% cardiomyocytes, parallel cultures treated with GCV or puromycin, respectively, contained 33.4 +/- 2.1% or 91.5 +/- 4.3% cardiomyocytes corresponding to an enrichment factor of 6.7- or 14.5-fold. Drug-selected cardiomyocytes responded to chronotropic stimulation and displayed cardiac-specific action potentials, demonstrating that functionality was retained. Both transgenic strategies will be generically applicable and should readily translate to the enrichment of many other differentiated lineages derived from hESCs.
Stem Cell Tourism and The Role of Health Professional Organizations
Authors: G. K. D. Crozier, Kyle Thomsen
Journal: The American Journal of Bioethics (2010)

Click to increase image sizeClick to decrease image size Notes 1. It is also worth noting that all information must be gathered in a noncoercive, voluntary fashion. It is likely that this will not be difficult, given that the patients are as starved for information as the physicians. Regardless, confidentiality must be respected. 2. AHRC's initiative was developed in conjunction with its January 2009 symposium: the "First Invitational International Forum on Cross-Border Reproductive Care: Quality and Safety" (www.ahrc-pac.gc.ca/doc.php?did=35&lang=eng).
Rocky Mountain Spotted Fever in the Mid-South.
Authors: J. Torres
Journal: Annals of Internal Medicine (1972)

Meeting Abstracts1 May 1972Rocky Mountain Spotted Fever in the Mid-South.J. Torres, M.D., E. Humphreys, M.D., A. Bisno, M.D., F.A.C.P.J. Torres, M.D.Search for more papers by this author, E. Humphreys, M.D.Search for more papers by this author, A. Bisno, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-76-5-873_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptContrary to the implication in its name, Rocky Mountain spotted fever occurs more frequently in the southeastern United States than in the mountain states. From 1 July 1965 to 31 August 1971, 42 patients with Rocky Mountain spotted fever were hospitalized in Memphis, Tenn.; this report is based on a retrospective analysis of their hospital records. Despite the occurrence of these cases during the warmer months and in an area where spotted fever is endemic; the correct diagnosis was often missed initially. In only 11 cases (26%) was Rocky Mountain spotted fever considered when the patients first came to medical... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Memphis, Tenn. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byInfectious diseases: annual review of significant publications. 1 May 1972Volume 76, Issue 5Page: 873-873KeywordsFeversRocky Mountain spotted fever Issue Published: 1 May 1972 PDF downloadLoading ...
THE EFFECT OF HIGH PROTEIN DIETS ON EXPERIMENTAL RENAL HYPERTENSION
Authors: H. Philipsborn, L. N. Katz, S. Rodbard
Journal: The Journal of Experimental Medicine (1941)

The effect of high and low protein diets were studied on fourteen dogs in twenty-four different experiments. In only two of these animals, both with moderate renal excretory failure, was a reversible rise in blood pressure elicited by a high protein diet. The possible mechanisms involved in meeting an increased excretory load are discussed.
BLOOD PHENOL LEVEL AFTER TOPICAL APPLICATION OF PHENOL-CONTAINING PREPARATIONS
Authors: Rudolph Ruedemann
Journal: JAMA (1953)

Calamine lotion, containing 1 or 2% phenol, and a preparation composed of phenol (4.75%) and camphor (10.86%) in aromatized liquid petrolatum<sup>1</sup>(camphophenique<sup>®</sup>) have been favorites for several decades for the treatment of disorders of the skin. Both preparations have been applied extensively with only a single incident of harmful effect reported for each. This is in contrast to the local changes, such as gangrene, and systemic effects that resulted frequently from application of aqueous solutions containing similar concentrations of phenol. Recently a fatality occurred because of the indiscriminate application of large volumes of a proprietary preparation containing 2.3% phenol in corn oil (foille<sup>®</sup>). This material was applied to a dressing covering 25 or 30% of a boy's body surface for the treatment of a kerosene burn.<sup>1</sup>The object of this investigation was to follow the level of "free" and of "conjugated" phenol of the blood after
Review of <i>Ethics in Nursing: Cases, Principles, and Reasoning</i>
Authors: Susan B. Fowler
Journal: JAMA (2011)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Journals JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2024 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
α Thalassemia—Caused by Gene Deletion
Authors: Z. Danilevicius
Journal: JAMA (1974)

The thalassemia syndromes are due to quantitative abnormalities of hemoglobin peptide synthesis. Thalassemia is manifested only by hypochromia and microcytosis, with little or no anemia in the heterozygous patient (minor form); however, it causes severe hypochromic, microcytic anemia with features of hemolysis in the homozygous patient (major form). Actually, thalassemia refers to a whole spectrum of genetically determined diseases of red blood cells (RBC). The primary defect is in the rate of hemoglobin synthesis. When the genetic anomaly depresses only β-globin chain synthesis, the disorder is termed β thalassemia. This type of thalassemia is expressed in both heterozygous and homozygous forms and comprises more than 90% of all cases. β thalassemia in homozygous patients has been known as Cooley anemia. From 5% to 10% of persons carrying the thalassemia trait have hemoglobin A<sub>2</sub>levels affected. These patients have an abnormal synthesis of hemoglobin due to a-globin deficiency or abnormality. The
[185] CENTRILOBULAR NECRO-INFLAMMATORY CHANGES IN HCV-NEGATIVE LIVER TRANSPLANT RECIPIENTS RESPOND TO TREATMENT WITH STEROIDS AND MYCOPHENOLATE MOFETIL
Authors: R. Vigano, S. Fagiuoli, A. Sonzogni, L. Pasulo, G. Gaffuri, M. Colledan, M. Strazzabosco
Journal: Journal of Hepatology (2007)
Interleukin-6–dependent survival of multiple myeloma cells involves the Stat3-mediated induction of microRNA-21 through a highly conserved enhancer
Authors: Dennis Löffler, Katja Brocke-Heidrich, Gabriele Pfeifer, Claudia Stocsits, Jörg Hackermüller, Antje K. Kretzschmar, Renate Burger, Martin Gramatzki, Conny Blumert, Kay Bauer, Helena Cvijic, A. Kerstin Ullmann, Peter F. Stadler, Friedemann Horn
Journal: Blood (2007)
CDC Plays Cat-and-Mouse With Flu Virus
Authors: Teri Randall
Journal: JAMA (1990)

AN INTERNATIONAL network of laboratories, coupled with a multifaceted surveillance program in the United States, is helping the CDC anticipate next year's dominant influenza virus strains in time for pharmaceutical firms to make an effective vaccine. The pharmaceutical companies' lengthy vaccine-manufacturing process mandates that researchers decide which strains should be included in the following year's vaccine while the nation still is in the midst of the current year's flu season. By mid-February, experts from the CDC and Food and Drug Administration provide their data to the Food and Drug Administration's vaccine advisory panel that makes the final recommendations for the US vaccine. The World Health Organization (WHO), an autonomous unit within the United Nations system with headquarters in Geneva, Switzerland, usually echoes these recommendations, based on CDC data as well as data collected by Mill Hill in London, the other WHO influenza collaborating center. Although the recommendations still harbor an
Reform of investigation of deaths
Authors: Richard Baker, Stephen Cordner
Journal: BMJ (2006)

example of the aesthetically pleasing vulva.We went to our patients for the answer and they said the playmates of Playboy." 10 In other words, women are being mutilated to fit male masturbation fantasies, in what Faith Wilding calls "the full-scale consumer spectacle of the cyborg porn babe." 11This burgeoning industry is able to operate without the slightest attention being paid to it by medical researchers.There is not a single reference to laser vaginoplasty on PubMed.The WHO definition of female genital mutilation is "all procedures involving partial or total removal of the external female genitalia or other injury to the female genital organs whether for cultural, religious, or other non-therapeutic reasons."It is Western medicine which, by a process of disease mongering, 12 is driving the advance of female genital mutilation by promoting the fear in women that what is natural biological variation is a defect, a problem requiring the knife.
THE SUPPLY OF MORPHINE, ETC., TO ARMY MEDICAL OFFICERS
Authors: W. Martin
Journal: BMJ (1917)

<h3>Abstract</h3> Studying viral antibody neutralization data is a complex task and knowledge relating to the effectiveness of a particular antibody to particular strains of viruses cannot easily be extrapolated to other new, related strains. We have developed ANNAVP, a software that uses neural networks to model viral protein data. ANNAVP uses supervised or unsupervised learning and viral protein sequence data to form correlations between different strains and to predict the effectiveness of neutralizing agents against them.
Tumor‐primed natural killer cells from patients with multiple myeloma lyse autologous, NK‐resistant, bone marrow‐derived malignant plasma cells
Authors: Eirini Katodritou, Evangelos Terpos, Janet North, Panagiotis Kottaridis, Evgenia Verrou, Vasiliki Gastari, Christina Chadjiaggelidou, Shivajanani Sivakumaran, Samuel Jide‐Banwo, Maria Tsirogianni, Dimitrios Kapetanos, Konstantinos Zervas, Mark W. Lowdell
Journal: American Journal of Hematology (2011)

Natural killer (NK) cells are cytotoxic lymphocytes able to kill tumor cells and virus-infected cells. Human-resting NK cells can be activated by co-culture with NK-resistant CTV-1a cells. These tumor-activated cells (TaNKs) are cytotoxic to a range of NK-resistant tumor cells in vitro. This potential, however, has not been explored in multiple myeloma (MM). In this study, we demonstrate that TaNK cells from 21 MM patients lyse a variety of myeloma targets, including primary isolates of autologous and allogeneic CD138+ myeloma cells whilst sparing CD138-ve bone marrow cells. Myeloma patients' TaNK-induced lysis of the U266 cell line was significantly higher compared to normal controls (median-specific lysis 79.1% vs. 69.5%) (P = 0.003). In addition, TaNKs induced substantial lysis of autologous and allogeneic CD138+ myeloma cells (median-specific lysis 52.5% and 37.4%, respectively). The percentage of specific lysis did not correlate with important disease characteristics (ISS, age, and high-risk molecular abnormalities) or with the disease status and antimyeloma treatment, including novel agents and dexamethasone. In conclusion, tumor-primed NK cells are able to induce substantial lysis of myeloma targets including autologous and allogeneic CD138+ myeloma plasma cells and could be an additional therapeutic approach in MM, particularly in the era of novel agents.
ASYMPTOMATIC INDIVIDUALS WITH NON-CALCIFIED PLAQUE ALONE BY CORONARY CT ANGIOGRAPHY: ASSOCIATION TO CORONARY ARTERY DISEASE RISK FACTORS AND PROGNOSTIC IMPLICATIONS FOR PATIENTS IN THE PROSPECTIVE MULTICENTER INTERNATIONAL CONFIRM REGISTRY
Authors: Stephan Achenbach, Allison Dunning, Daniel Berman, Matthew Budoff, Tracy Callister, Hyuk-Jae Chang, Victor Cheng, Augustin DeLago, Martin Hadamitzky, Joerg Hausleiter, Ronald Karlsberg, Philipp Kaufmann, Fay Lin, Mouaz Al-Mallah, Khurram Nasir, Leslee Shaw, Todd Villines, James Min
Journal: Journal of the American College of Cardiology (2012)
Malignant Glioma and ABO Blood Group
Authors: P. O. Yates
Journal: BMJ (1964)

MEDIC"OURNAL of synergistic gangrene is by a wide excision ,of the affected area.In contrast to the acutely ill patient with streptococcal gangrene, the victim of synergistic gangrene has no _pyrexia, nor a toxic state until a very late stage.The main features of synergistic gan- grene are severe pain, exquisite tenderness, and marked psychological depression.As the lesion develops the appearance is that of a necrotic area of skin and subcutaneous fat enclosed by a rim of blackened skin.In turn the black rim is encompassed by a zone of hyperaemia.Coloured films of the established lesion were taken in a patient who came under my -care.As the condition is rare, anyone inter- ested in the appearances is welcome to a loan of the slides.-Iam, etc.,
High Levels of High Sensitivity C-Reactive Protein and Uric Acid can Predict Disease Severity in Patients with Mitral Regurgitation
Authors: Yasin Türker, Ismail Ekinozu, Yusuf Aslantas, Yasemin Turker, Mehmet Akkaya, Melih Engin Erkan
Journal: Journal of the American College of Cardiology (2013)
Vitamin C and vascular disease
Authors: C. J Bulpitt
Journal: BMJ (1995)

Chamberlain, who was also Pearce's head of department and editor of the journal, had twice asked for his name to be removed.Of all the abuses of scientific research, gift authorship is the most common and the most lightly regarded.Even the royal college's report, in comments that I disagree with, states that "Mrs Hamid's contributions...in the way of literature searches and writing of introduction and discussion components...justified her acceptance of coauthorship" and "Mr Manyonda's contribution . . .was at an intellectual level with significant contribution to the discussion ... there is no
Biomagnetometry provides a new compass for exploring the brain and heart
Authors: A. Skolnick
Journal: JAMA (1990)

AMONG  more than 1400 papers presented at  75th Scientific Assembly and Annual Meeting of  Radiological Society of North America (RSNA), there was only one on biomagnetic imaging. Nevertheless, in  opinion of a number of radiologists there—including Derek Harwood-Nash, MD, who is  RSNA's program committee chair— this fledgling field is one of  most exciting areas in radiology today, with many potential diagnostic applications in cardiology, neurology, and psychiatry.  At  next few gatherings of the world's largest medical meeting, he expects to see many more papers on biomagnetic imaging that should provide a clearer view of what this latest round of ultra-high technology (and ultra-high cost) imaging can do for biomedical research and patient care.  Detailing Electrical Activity  Unlike ultrasound, computed tomography, and magnetic resonance imaging (MRI) scans (which provide anatomic information) and positron emission tomography and single-photon computed tomography scans (which show metabolic information),
Tenofovir discontinuation could predispose to urolithiasis in atazanavir-treated patients
Authors: Massimiliano Fabbiani, Laura Bracciale, Maria Doino, Alessandro D’Avino, Angela Marzocchetti, Pierluigi Navarra, Roberto Cauda, Andrea De Luca, Simona Di Giambenedetto
Journal: Journal of Infection (2011)
Prognostic Value of the Human Kallikrein Gene 15 Expression in Ovarian Cancer
Authors: George M. Yousef, Andreas Scorilas, Dionyssios Katsaros, Stefano Fracchioli, Lisa Iskander, Carla Borgono, Irene A. Rigault de la Longrais, Manuela Puopolo, Marco Massobrio, Eleftherios P. Diamandis
Journal: Journal of Clinical Oncology (2003)

KLK15 is a newly cloned human kallikrein gene. Many kallikreins were found to be differentially expressed in ovarian cancer. Like other kallikreins, KLK15 is regulated by steroid hormones in cancer cell lines. KLK15 is upregulated mainly by androgens and to a lesser extent by progestins. The purpose of this study was to examine the prognostic value of KLK15 in ovarian cancer tissues.We studied KLK15 expression by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in 168 consecutive patients with epithelial ovarian cancer. Ten patients with benign ovarian tumors were also included in the study. An optimal cutoff point equal to the 50th percentile was defined based on the ability of KLK15 to predict progression-free survival and overall survival of the study population.KLK15 expression levels were significantly higher in cancerous tissues compared with benign tumors. Kaplan-Meier survival curves showed that KLK15 overexpression is a significant predictor of reduced progression-free survival (PFS; P <.001) and overall survival (OS; P <.009). Univariate and multivariate analyses indicate that KLK15 is an independent prognostic factor for PFS and OS. A weak positive correlation was found between KLK15 expression and serum CA-125 levels.KLK15 expression, as assessed by quantitative RT-PCR, is an independent marker of unfavorable prognosis for ovarian cancer.
Paget's disease and primary hyperparathyroidism.
Authors: E Lester
Journal: BMJ (1978)

HLA patterns and diabetic retinopathyThe major cauise of morbidity in insulin-dependent diabetic patients is vascular complications-particularly microangiopathy, as reflected by retinopathy and nephropathy.The factors influencing the development of these complications remain poorly understood.Studies in diabetic identical twins have suggested that genetic factors may have a role not only in the liability to diabetes but also in the likelihood of diabetics developing retinopathy.1The pattern of HLA antigens is genetically determined and an association between the HLA pattern and several diseases, including insulin-dependent diabetes, has been well established.This investigation aimed at seeking an association between the HLA pattern and liability to or protection from severe diabetic retinopathy in insulin-dependent diabetic patients.Because of a recent report in abstract form,2 we directed our attention to the antigens HLA-A1 and HLA-B8.
Prevalence of atopic disorders among adolescents in Turku, Finland
Authors: E. Varjonen, K. Kalimo, K. Lammintausta, P. Terho
Journal: Allergy (1992)

A study of the prevalence atopic disorders among 15–16‐year‐old teenagers was carried out in a coastal urban town in south‐western Finland. Altogether, 1712 children were found in that age group, all previously examined by a pediatrician. Each child who had present or previous allergic diseases was invited for a detailed study, a total of 434 (25%) pupils. Of these patients 416 (95.8%) participated in clinical examination and skin testing. The prevalence of atopic diseases was 21% in the studied group; atopic eczema was found in 9.7%, allergic rhinitis in 14%, and asthma in 2.5%. Of subjects who had rhinitis, 38% also had atopic eczema, while rhinitis ‐ as the only symptom ‐was found in 8.8%. Figures obtained from this survey suggest that the prevalence rates of atopic diseases are about the same as found 10 years age in Finland and they correspond also with other recent reports.
Molecular Mechanisms of Immunosuppression and Anti-inflammatory Activities by Glucocorticoids
Authors: Joseph A. Didonato, Fahri Saatcioglu, Michael Karin
Journal: American Journal of Respiratory and Critical Care Medicine (1996)

"Molecular Mechanisms of Immunosuppression and Anti-inflammatory Activities by Glucocorticoids." American Journal of Respiratory and Critical Care Medicine, 154(2_pt_2), pp. S11–S15
Fetal hemoglobin in women with normal and with hydatidiform molar pregnancy
Authors: John C. Lee, Robert H. Hayashi, Marguerite K. Shepard
Journal: American Journal of Hematology (1982)

Abstract The quantity and the rate of synthesis of fetal hemoglobin (HbF); the level of HbF‐containing erythrocytes (F cells); and the levels of progesterone, human chorionic gonadotropin (HCG), and human chorionic somatomammotropin (HCS) in serial blood samples from women at different stages of pregnancy were determined. An increase was observed in the synthesis and the quantity of maternal HbF, reaching a peak at about 9–12 weeks gestation. A major peak in F‐cell level was also detected at about 9–12 weeks, with two minor peaks appearing at about 16–20 and 22–24 weeks. Levels of HCG, HCS, and progesterone also varied during pregnancy. Timing of the rise and fall of HCG levels corresponded to that of maternal HbF levels, whereas levels of HCS and progesterone did not reach their peak until later stages of gestation when HbF level had returned to normal. Similar analysis conducted on patients with hydatidiform molar pregnancy revealed that the majority (&gt;95%) of these patients showed significant increases in HbF synthesis at the time of molar evacuation, but decreased to normal levels in approximately 30 days postevacuation. A similar profile in the levels of HCG, but not progesterone and HCS, was observed. A positive correlation between levels of HCG and HbF can be established (correlation coefficient = 0.94). These data are in agreement with the hypothesis that HCG may act as a stimulus for the enhanced HbF synthesis in adults.
Endoscopic Management of Rare Congenital Malformations of the Male Urethra
Authors: C. Schulman, F. D'Udekem
Journal: Endoscopy (1977)

Partial duplication of the urethra and dilatation of Cowper's duct are very rare congenital anomalies of the lower urinary tract which can be diagnosed and located by endoscopy. Treatment consists in transurethral resection of the septum between the urethra and the accessory channel.