tbuckley/cabot-search huggingface dataset:
MBASED: allele-specific expression detection in cancer tissues and cell lines
Authors: Oleg Mayba, Houston N Gilbert, Jinfeng Liu, Peter M Haverty, Suchit Jhunjhunwala, Zhaoshi Jiang, Colin Watanabe, Zemin Zhang
Journal: Genome biology (2014)

Allele-specific gene expression, ASE, is an important aspect of gene regulation. We developed a novel method MBASED, meta-analysis based allele-specific expression detection for ASE detection using RNA-seq data that aggregates information across multiple single nucleotide variation loci to obtain a gene-level measure of ASE, even when prior phasing information is unavailable. MBASED is capable of one-sample and two-sample analyses and performs well in simulations. We applied MBASED to a panel of cancer cell lines and paired tumor-normal tissue samples, and observed extensive ASE in cancer, but not normal, samples, mainly driven by genomic copy number alterations.
Why pay comparisons do not always help
Authors: W. Russell
Journal: BMJ (1982)

Industrial muscle is usually provided by numbers, but in the case of top people it is the reverse.Giving them their money does' not actually cost all that much.The point was clearly made by the Top Salaries Review Body (TSRB) in its recent report.It pointed out that the grades of civil servants that it deals with account for about 0 5%', of the basic salary bill of the non-industrial Civil Service as a whole, or about L17m out of £3300m.The rises proposed by the TSRB for top civil servants, senior members of the armed Forces, and the judiciary ranged on average between 19-4'%, and 24 3',.They were cut by the
Combined use of narrow-band imaging magnification endoscopy and miniprobe confocal laser microscopy in neoplastic Barrett's esophagus
Authors: S. Miehlke, A. Morgner, D. Aust, A. Madisch, M. Vieth, G. Baretton
Journal: Endoscopy (2007)

The identification of intraepithelial neoplasia in Barrett’s esophagus in cases where there are no visible focal lesions remains a challenge. Novel techniques such as narrow-band imaging-assisted magnification endoscopy or confocal laser microscopy could, however, improve the endoscopic detection of neoplasia in Barrett’s esophagus [1,2]. A miniprobe-based confocal laser microscopy system (Cellvizio-GI; Mauna Kea Technologies, Paris, France) became available recently which allows the application of laser microscopy with any conventional endoscope and mosaic imaging [3,4].
Modest increase seen in US health spending in 2010
Authors: B. Roehr
Journal: BMJ (2012)

Total health spending in the United States reached US $2.6 trillion (£1.7 trillion; €2 trillion) in 2010, or US $8402 per person, according to the latest annual report from the actuary office of the federal government’s Centers for Medicare and Medicaid Services (CMS). That is an increase from US $2.5 trillion (US $8086 per person) the previous year.

A report, published in the journal Health Affairs , shows that the rate of increase last year was 3.9%, compared with 3.8% in 2009. The rates of increase in 2009 and 2010 were the lowest in the five decades that the report has been issued.

Health spending as a percentage of the …
Identifying HIV patients with an unfavorable cardiovascular risk profile in the clinical practice: Results from the SIMONE study
Authors: Giuseppe Vittorio L. De Socio, Giustino Parruti, Tiziana Quirino, Elena Ricci, Giuseppe Schillaci, Beatrice Adriani, Patrizia Marconi, Marzia Franzetti, Canio Martinelli, Francesca Vichi, Giovanni Penco, Claudio Sfara, Giordano Madeddu, Paolo Bonfanti
Journal: Journal of Infection (2008)
Adherence of Malassezia isolates to human keratinocytes in vitro — a study of HIV-positive patients with seborrhoeic dermatitis
Authors: R.C. SCHECHTMAN, G. MIDGLEY, J.S. BINGHAM, R.J. HAY
Journal: British Journal of Dermatology (1995)

Adherence of Malassezia yeast cells to human keratinocytes was assessed by a novel technique using double‐aided Sellotape. Although adherence using double‐sided Sellotape is still merely a model for in vivo adherence, it approximates to the conditions found on the skin surface. There were no differences in adhesive properties to human keratinocytes between Malassezia strains originating from HIV‐positive and HIV‐negative patients with seborrhoeic dermatitis, nor was there a relationship between the severity of seborrhoeic dermatitis and in vitro adherence to human keratinocytes.
Evidence of Bacterial Metabolic Activity in Culture-Negative Otitis Media With Effusion
Authors: Mark G. Rayner
Journal: JAMA (1998)

Context.—Otitis media with effusion (OME) can lead to significant hearing loss in children. Although previous studies have shown that bacterial DNA is present in a significant percentage of effusions sterile by culture, whether the DNA represents viable organisms or "fossilized remains" is unknown.Objective.—To determine if bacterial messenger RNA (mRNA), as detected by a reverse transcriptase–polymerase chain reaction (RT-PCR)–based assay, is present in chronic pediatric middle ear effusions that contain bacterial DNA but are sterile by standard cultural methods. Bacterial mRNAs have a half-life measured in seconds to minutes; therefore, detection of bacteria-specific mRNAs would be evidence that metabolically active organisms are present.Design.—Blinded comparative study.Patients.—A total of 93 effusions from pediatric outpatients seen for myringotomy and tube placement for chronic (&gt;3 months) OME (median age of children, 17 months).Setting.—Tertiary care pediatric hospital.Main Outcome Measures.—Percentage of positive test results for RT-PCR–based assays compared with culture for Haemophilus influenzae and concordance between RT-PCR and PCR-based findings for bacterial nucleic acids.Results.—Eleven (11.8%) of the 93 specimens tested positive by culture, PCR, and RT-PCR for H influenzae. A total of 29 specimens (31.2%) were positive by PCR but negative by culture for H influenzae. All 29 specimens were positive by RT-PCR for H influenzae–specific mRNA.Conclusions.—The RT-PCR–based assay system can detect the presence of bacterial mRNA in a significant percentage of culturally sterile middle ear effusions, establishing the presence of viable, metabolically active, intact organisms in some culture-negative OME.
PROBLEMS IN APPLICATION OF THE BASIC CRITERIA OF SCHIZOPHRENIA
Authors: HOWARD N. COOPER
Journal: American Journal of Psychiatry (1960)

Four cases were diagnosed as schizophrenic by careful observation and documentation. The patients had the following in common: 1. By all symptoms no schizophrenia was immediately apparent. It required time and attention to establish the diagnosis in all cases. But then it remained firmly established by the basic accepted criteria. 2. The patients were persons of good intelligence and had other assets as well. They tended to mobilize many mechanisms of defense as "ego" structure threatened to "weaken" further. These consisted both of additional neurotic defenses and some tendency toward "acting-out," but no change was manifested in areas considered schizophrenic. Therefore, we are considering a category of individuals who belong to the group of schizophrenias according to legitimate definition, but apparently no more capable of disorganization, or display of blatant "accessory" symptoms, than the next person. In each of these instances, schizophrenic psychopathology comprised one part of the total picture, but did not thereby characterize the case. This concept differs from Hoch's "pseudoneurotic schizophrenia" (9) in that the latter describes an illness which is schizophrenic for all important clinical purposes: disorganization potential, therapeutic technique required, goals of therapy, and prognosis. By way of contrast, perhaps the best diagnostic approach to the above "pseudoschizophrenic neurotic" cases is the one or two paragraph description of psychopathology, with all factors evaluated on an individual basis.
Effects of phenobarbital and rose bengal on the ATPases of plasma membranes of rat and rabbit liver
Authors: Y. Laperche, A. Launay, P. Oudea
Journal: Gut (1972)

The effects of drugs which change the bile-salt-independent fraction of bile flow on Na+Ka+ and Mg2+ activated ATPases were studied in membrane fractions rich in bile canaliculi. The administration of phenobarbital caused no induction of these enzymes which could explain the increased bile flow observed in the rat. Rose bengal, in addition to its strong photoxidative inhibition of both ATPases, inhibits the Na+K+ ATPase of rat and rabbit bile canaliculi in the absence of light. A closely related substance, uranine, inhibits neither bile flow nor Na+K+ATPase. Inhibition of this enzyme by rose bengal may therefore be responsible for the observed effects of this dye on bile flow independent of bile salts.
JFK's assassination. Conspiracy, forensic science, and common sense
Authors: R. R. Artwohl
Journal: JAMA (1993)

The Journalinterviews with Humes and Boswell,<sup>1</sup>the Dallas physicians,<sup>2</sup>and Finck<sup>3</sup>cleared up many of the events surrounding the attempted resuscitation and subsequent autopsy of President Kennedy. However, correspondence toThe Journalindicates many physicians are still sympathetic to a key proconspiracy tenet regarding the Kennedy assassination: that the autopsy physicians conspired with the military, the Central Intelligence Agency (CIA), the Federal Bureau of Investigation (FBI), the Secret Service, and other agencies of government to disguise and suppress medical evidence that would show President Kennedy was publicly executed in Dealey Plaza on November 22,1963, by multiple gunmen. Unfortunately, many of the arguments raised in the letters supporting such a complex conspiracy went unanswered. These arguments should be addressed in<i>JAMA</i>for several reasons. See also pp 1507, 1544 and 1552. First, for many physicians, the only contact they will have with arguments for and against conspiracy
ANTIHISTAMINES AS LOCAL ANESTHETIC AGENTS FOR URETHRAL MANIPULATION
Authors: Raymond J. Fitzpatrick
Journal: JAMA (1952)

Not infrequently patients are encountered who manifest sensitivity to local anesthetic agents. The urethral mucous membrane is a very effective absorbing surface, and poisoning has frequently occurred from the application of local anesthetics. The rapidity of absorption is well illustrated by the fact that the injection of 1% apomorphine into the urethra of the male dog results in vomiting in three to five minutes. In the past many patients have been seen who demonstrated atopic responses to the extent that it became necessary to carry out urethral manipulations without the aid of any anesthetic agents. Having learned of the anesthetic action produced by the antihistamines when applied topically in other specialized fields of work such as gastroenterology and dentistry, we made an effort to determine their usefulness from the standpoint of surface anesthesia in the urological patient. <h3>PRIOR STUDIES</h3> Rosenthal and Minard<sup>1</sup>are credited with being the first to
Insulin Therapy for the Management of Diabetic Ketoacidosis
Authors: Andrea Granados, Joyce M. Lee
Journal: JAMA Pediatrics (2014)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Pediatrics HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Pediatrics journal
Influence of highly purified eicosapentaenoic acid ethyl ester on insulin resistance in the Otsuka Long-Evans Tokushima Fatty Rat, a model of spontaneous non-insulin-dependent diabetes mellitus
Authors: Yutaka Mori, Yūichi Murakawa, Shūichi Katoh, Shōichi Hata, Junichi Yokoyama, Naoko Tajima, Yoshio Ikeda, Hidefumi Nobukata, Tsutomu Ishikawa, Yasunori Shibutani
Journal: Metabolism (1997)
Role of p21-activated kinase pathway defects in the cognitive deficits of Alzheimer disease
Authors: Lixia Zhao, Qiu-Lan Ma, Frédéric Calon, Marni E Harris-White, Fusheng Yang, Giselle P Lim, Takashi Morihara, Oliver J Ubeda, Surendra Ambegaokar, James E Hansen, Richard H Weisbart, Bruce Teter, Sally A Frautschy, Greg M Cole
Journal: Nature Neuroscience (2006)
The morphological and histological findings of restenosis after self-expanding biodegradable coronary stent implantation
Authors: Takafumi Tsuji, Hideo Tamai, Keiji Igaki, Eisho Kyo, Kunihiko Kosuga, Tatsuhiko Hata, Masaharu Okada, Takuji Nakamura, Sinya Fujita, Sinsaku Takeda, Seiichiro Motohara, Hiromu Uehata
Journal: Journal of the American College of Cardiology (2002)
Adult linear IgA disease and chronic bullous disease of childhood: the association with human lymphocyte antigens Cw7, B8, DR3 and tumour necrosis factor influences disease expression
Authors: P.M. Collier, F. Wojnarowska, K. Welsh, W. Mcguire, M.M. Black
Journal: British Journal of Dermatology (1999)

Linear IgA disease and chronic bullous disease of childhood are both subepidermal autoimmune blistering diseases. Class I and II major histocompatibility locus (MHC) antigen typing was performed on 60 patients (26 chronic bullous disease of childhood, 34 adult linear IgA disease), and the findings were correlated with the clinical course. The typing was performed using a lymphocyte microcytotoxicity assay, and the results were compared with a reference population of U.K. organ donors. Analysis of the tumour necrosis factor (TNF) locus was performed using sequence-specific oligonucleotides on a dot blot in 51 patients and compared with a random control population and human lymphocyte antigen (HLA) DR3 matched controls. The disease was found to be significantly associated with HLA Cw7 (chi2 = 19.24, P = 0.001), B8 (chi2 = 9.89, P = 0.04) and DR3 (chi2 = 10.47, P = 0.014), all components of the common Caucasian haplotype. There was also a close association between the disease and possession of HLA DR2 or 3 (chi2 = 16.34, P = 0.001). A reduction in the incidence of DR1 and DR4 (alleles carrying the rheumatoid motif) was observed, which is more marked in the children (chi2 = 8.34, P = 0.039). In the childhood group there was an increased frequency of B8, DR3 and DQ2 compared with the adults which included five of 26 who were homozygous for these antigens, a feature not seen in the adults, which may account for the differences seen between the two groups. Possession of HLA B8, DR3 and DQ2 probably facilitates earlier presentation of the disease as there is no evidence from our results that the adults and children differ fundamentally in their MHC associations. The rare TNF2 allele was found in 29 of 51 patients (expected 8.2, chi2 = 18. 3, P = 0.0001). This was more marked in the children (19 of 26). Patients with the TNF2 allele had a longer disease duration (5.3 years TNF2, 3.0 years TNF1).
Correction of B-cell development in Btk-deficient mice using lentiviral vectors with codon-optimized human BTK
Authors: Y Y Ng, M R M Baert, K Pike-Overzet, M Rodijk, M H Brugman, A Schambach, C Baum, R W Hendriks, J J M van Dongen, F J T Staal
Journal: Leukemia (2010)
The Code Revision.—An Appeal to Men of Good Sense.
Authors: John G. Truax
Journal: JAMA (1894)

away.Its revival may be due to the genius of the Majority Committee.Not one sentence in this first Article has a legit¬ imate place in any code of ethics.So much for the Com¬ mittee's method of "revising" the American Code of Ethics! 7. Xs, these two letters, long ago united in good literary faith, were finally discovered to be incompatible and were divorced by an act of literature.They are now recoupled for the sole purpose of symbolizing the unknown quantity and superfluity in concubinage.Xs would be an appropri¬ ate device for the signet of the Code "revising " committee
Depression Screening in Patients With Heart Disease—Reply
Authors: Brett D. Thombs
Journal: JAMA (2009)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Non-doctor in the house
Authors: I. Iheanacho
Journal: BMJ (2009)

What exactly is a doctor? Beyond their common gestation in medical school, what description embraces the roles and responsibilities of, say, the paediatric oncologist and the rural GP, the forensic psychiatrist and the hand surgeon, while strictly excluding other healthcare professions? Coming up with a succinct definition for something so seemingly fundamental is difficult.

Maybe this didn’t matter as much when the place of doctors was easier to recognise or assume, and harder to challenge. Once upon a time, such people were more easily identifiable …
Delayed Air Embolism After Removal of Venous Catheters
Authors: PETER C. HANLEY
Journal: Annals of Internal Medicine (1984)

Letters and Corrections1 September 1984Delayed Air Embolism After Removal of Venous CathetersPETER C. HANLEY, M.D., ROGER L. CLICK, M.D., ROBERT G. TANCREDI, M.D.PETER C. HANLEY, M.D.Search for more papers by this author, ROGER L. CLICK, M.D.Search for more papers by this author, ROBERT G. TANCREDI, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-101-3-401_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: We wish to alert physicians and others who are involved in central venous catheterization and catheter care to the possibility of venous air embolism occurring along the subcutaneous track after removal of the catheter from the central vein.Recently, we cared for a 76-year-old man who had had an anterolateral myocardial infarction and required hemodynamic monitoring. A catheter sheath was easily positioned at the first attempt in the right internal jugular vein (by the anterior approach) without complication, and a Swan-Ganz catheter was introduced through this sheath. Three days after insertion, the catheter and sheath were removed...References1. PASKINHOFFMANTUDDENHAM DWW. A new complication of subclavian vein catheterization. Ann Surg. 1974;179:266-8. CrossrefMedlineGoogle Scholar2. O'QUINLAKSHMINARAYAN RS. Venous air embolism. Arch Intern Med. 1982;142:2173-6. CrossrefMedlineGoogle Scholar3. BRECHNERBETHUNE VR. Recent advances in monitoring pulmonary air embolism. Anesth Analg. 1971;50:255-61. CrossrefMedlineGoogle Scholar4. TUNNICLIFFESTEBBING FG. The intravenous injection of oxygen gas as a therapeutic measure. Lancet. 1916;2:321-3. CrossrefGoogle Scholar5. YEAKEL A. Lethal air embolism from plastic blood-storage container. JAMA. 1968;204:267-9. CrossrefMedlineGoogle Scholar6. CAMPS F. Gradwohl's Legal Medicine. 3rd ed. Bristol: John Wright & Sons; 1976:461-2. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Mayo Clinic and Mayo Foundation Rochester, MN 55905 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByNear-fatal Air Embolism: Fibrin Sheath as the Portal of Air EntryLethal air embolism following removal of a double lumen jugular vein catheterEmbolie gazeuse cérébrale après retrait d'un cathéter jugulaire interneEmbolie gazeuse iatrogèneCentral venous cannulationInternal Jugular Vein Cannulation for HemodialysisCentral Venous Catbeterization: A Comprehensive Review, Part II 1 September 1984Volume 101, Issue 3Page: 401-402KeywordsCatheterizationCathetersHemodynamicsJugular veinMyocardial infarctionVeins Issue Published: 1 September 1984 PDF DownloadLoading ...
K+ currents and K+ channel mRNA in cultured atrial cardiac myocytes (AT-1 cells).
Authors: T Yang, M S Wathen, A Felipe, M M Tamkun, D J Snyders, D M Roden
Journal: Circulation Research (1994)

Atrial tumor myocytes derived from transgenic mice (AT-1 cells) maintain a well-differentiated cardiac biochemical and histological phenotype. In addition, they beat spontaneously in culture and exhibit long action potentials whose repolarization resembles that observed in native mammalian myocytes. In this study, we identified the major depolarization-activated outward currents in AT-1 cells; also, the presence of mRNAs that encode outwardly conducting ion channels was determined by cloning from an AT-1 cDNA library or by Northern hybridization. Among K+ channel isoforms, Kv2.1, minK, and Kv1.4 were readily detected in tumors and at 1 day in culture. Their abundance remained relatively stable (twofold or less change) after 14 days. The major outward current in AT-1 cells is a delayed rectifier that displays prominent inward rectification, activates rapidly (eg, 182 +/- 27 milliseconds [mean +/- SEM] at + 20 mV, n = 12), exhibits biexponential deactivation kinetics, and is extremely sensitive to the methanesulfonanilide dofetilide (IC50, 12 nmol/L). These characteristics identify this current as IKr, a delayed rectifier observed only in cardiac cells. IKr in AT-1 cells displayed slow inactivation: dofetilide-sensitive deactivating tails were greater after 1-second than after 5-second pulses. When IKr was blocked by &gt; or = 0.5 mumol/L dofetilide, time-independent current was usually recorded (50 of 65 experiments); rapidly inactivating (6 of 65) or slowly inactivating (9 of 65) outward currents were occasionally observed. We conclude that AT-1 cells express mRNAs encoding cardiac K+ channels and display a cardiac electrophysiological phenotype.(ABSTRACT TRUNCATED AT 250 WORDS)