tbuckley/cabot-search huggingface dataset:
Cell cycle effects resulting from inhibition of hepatocyte growth factor and its receptor c-Met in regenerating rat livers by RNA interference
Authors: Shirish Paranjpe, William C. Bowen, Aaron W. Bell, Kari Nejak-Bowen, Jian-Hua Luo, George K. Michalopoulos
Journal: Hepatology (2007)

Hepatocyte growth factor (HGF) and its receptor c-Met are involved in liver regeneration. The role of HGF and c-Met in liver regeneration in rat following two-thirds partial hepatectomy (PHx) was investigated using RNA interference to silence HGF and c-Met in separate experiments. A mixture of 2 c-Met-specific short hairpin RNA (ShRNA) sequences, ShM1 and ShM2, and 3 HGF-specific ShRNA, ShH1, ShH3, and ShH4, were complexed with linear polyethylenimine. Rats were injected with the ShRNA/PEI complex 24 hours before and at the time of PHx. A mismatch and a scrambled ShRNA served as negative controls. ShRNA treatment resulted in suppression of c-Met and HGF mRNA and protein compared with that in controls. The regenerative response was assessed by PCNA, mitotic index, and BrdU labeling. Treatment with the ShHGF mixture resulted in moderate suppression of hepatocyte proliferation. Immunohistochemical analysis revealed severe suppression of incorporation of BrdU and complete absence of mitosis in rats treated with ShMet 24 hours after PHx compared with that in controls. Gene array analyses indicated abnormal expression patterns in many cell-cycle- and apoptosis-related genes. The active form of caspase 3 was seen to increase in ShMet-treated rats. The TUNEL assay indicated a slight increase in apoptosis in ShMet-treated rats compared with that in controls. Conclusion: The data indicated that in vivo silencing of c-Met and HGF mRNA by RNA interference in normal rats results in suppression of mRNA and protein, which had a measurable effect on proliferation kinetics associated with liver regeneration. (Hepatology 2007.)
TWO CASES OF PERIPHERAL GANGRENE ASSOCIATED WITH LOCALIZED DISEASE OF THE ARTERIES.
Authors: HARRY M. SHERMAN
Journal: JAMA (1902)

<h3>HISTORY OF CASE ONE.</h3><h3>Case 1.</h3> —By Dr. Sherman. The patient is a man aged about 40. He has had no serious illness since childhood, when he had an attack of scarlatina, following which he was unable to walk for four months. He has not had syphilis. He has some dry eczema upon his forehead and about the joints. He eats well. His bowel and bladder functions are normal and he sleeps well. He has no sugar nor albumin in his urine. He gives a long account of the conditions which led to the removal of his left leg. About 1878, when it was the fashion to wear low shoes with large buckles, he had a severe pain at the top of the left instep and so discarded his buckle shoes. For some time he was then free from pain, but after awhile it returned with greater severity; it was
Idiopathic chondrolysis of the hip: a case report and review of the literature.
Authors: A W Hughes
Journal: Annals of the Rheumatic Diseases (1985)

A case of idiopathic chondrolysis of the hip in an adolescent Indian girl is reported. The association between idiopathic chondrolysis of the hip and primary protrusio acetabuli (Otto9s pelvis) is discussed.
Another deadly Zairian disease
Authors: J Burdon
Journal: BMJ (1996)

I first saw him on a Thursday afternoon, newly admitted from the clinic that morning. He was 22 and looked as if the was about to die. He weighed 40 kg and had lost 33% of his body weight over the previous 12 months. He was suffering from severe chronic diarrhoea, and when I examined him he had oral candida. The nurse who had admitted him from the outpatient department had written “Bangui” as the likely diagnosis in the notes. Bangui is a local euphemism for HIV infection, so named after the capital city of the Central African republic where one of the early international meetings on HIV was held. I sighed at seeing another life ending prematurely and at the prospect of having to spend time in an already busy day counselling him before and after performing the HIV test that would undoubtedly show him to be infected.

Each week we see about 10 new adult patients with HIV admitted to the ward. They present in a variety of ways but mostly with weight loss, fever, cough, or diarrhoea. Some, particularly those with tuberculosis, respond well to treatment and it is satisfying to follow them in the clinic as their weight increases, their cough diminishes, and their confidence is restored. But it is unrewarding work on the whole, and you feel that you are scratching the surface of the problem–indeed, …
The Synthesis of Acute Rheumatism
Authors: B. W. Foster
Journal: BMJ (1871)

IT is well known that, in cases of aneurism of the arch of the aorta, symptoms referable to the air-passages not unfrequently arise.The diagnosis of these cases is often much facilitated by the use of the laryngoscope.Such a case I saw lately with my friend and former pupil, Dr. Richards; the patient being under the joint care of Drs.Butler and Richards, of Winchester.A gentleman, Mr. H., aged 33, had noticed occasionally for a year past that, after running hard, his breathing became short, and attended with a noise in the throat.About six weeks before I saw him, while running after a wounded hare, his breathing became so difficult that he could run no longer, and he was nearly suffocated.At the time of his visit to me, on the 30th October, the:chief symptoms were cough, stridu-
Interleukin-10 prevents spontaneous death of germinal center B cells by induction of the bcl-2 protein.
Authors: Y Levy, J C Brouet
Journal: Journal of Clinical Investigation (1994)

In this study, we show that IL-10 enhances in vitro the viability of purified splenic B cells. There was a two- to threefold increase in recovery of viable cells during a 15-d culture period in the presence of IL-10. This effect was abolished by neutralizing antibodies to IL-10. The survival of large splenic B cells, which mostly represent follicular center cells, was similarly increased. The in vitro rescue from spontaneous death of the latter cells is known to involve a bcl-2-dependent pathway. We therefore investigated whether IL-10 might affect bcl-2 expression. Unseparated B cells as well as large splenic B cells displayed a strong expression of bcl-2 protein by immunofluorescence at days 2-7 of culture in the presence of IL-10. Other lymphokines such as IL-2 and IL-4 were able to trigger only a transient and faint expression of bcl-2; moreover, this effect was abolished by anti-IL-10 mAb. Inasmuch as activated B cells can produce their own IL-10, this lymphokine may play a crucial role in relieving from apoptosis those B cells that encounter their antigen in B cell follicles.
Polyarthritis and Neutropenia
Authors: KAMAL M. BAKRI
Journal: Annals of Internal Medicine (1986)

Letters and Corrections1 January 1986Polyarthritis and NeutropeniaKAMAL M. BAKRI, M.D.KAMAL M. BAKRI, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-104-1-127_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: In the September issue, Wallis and colleagues (1) describe the association of polyarthritis and neutropenia associated with circulating large granular lymphocytes. They state, "Polyarthritis has been described in only 3 patients." However, they have not accounted for two previous reports, namely, a patient described by Hooks and colleagues (2) and one reported by us (3). While studying our patient, we realized that this disease is a new and interesting entity, yet to be explored. We named it "RATS" syndrome (rheumatoid arthritis and T-suppressor lymphocytosis with neutropenia) and stressed its similarity to Felty's syndrome (3).Peripheral blood lymphocytes...References1. WALLISLOUGHRANKADINCLARKSTARKEBAUM WTMEG. Polyarthritis and neutropenia associated with circulating granular lymphocytes. Ann Intern Med. 1985;103:357-62. LinkGoogle Scholar2. HOOKSHAYNESHOOKS JBB. Gamma (immune) interferon production by leukocytes from a patient with a TG cell proliferative disease. Blood. 1982;59:198-201. CrossrefMedlineGoogle Scholar3. BAKRIEZDINLIWASSER KEL. T-suppressor cell chronic lymphocytic leukemia: phenotypic characterization by monoclonal antibodies. Cancer. 1984;54:284-92. CrossrefMedlineGoogle Scholar4. KRONENBERG L. Cellular effects of interferon, pp 80-2. In: STIEHM ER, moderator. Interferon: immunobiology and clinical significance. Ann Intern Med. 1982;96:80-93. MedlineGoogle Scholar1. WALLISLOUGHRANKADINCLARKSTARKEBAUM WTMEG. Polyarthritis and neutropenia associated with circulating granular lymphocytes. Ann Intern Med. 1985;103:357-62. LinkGoogle Scholar2. HOOKSHAYNESHOOKS JBB. Gamma (immune) interferon production by leukocytes from a patient with a TG cell proliferative disease. Blood. 1982;59:198-201. CrossrefMedlineGoogle Scholar3. DRAVESLOUGHRANCLARK KTE. Induction of natural killer activity in large granular lymphocyte leukemia with anti-C3 monoclonal antibodies [Abstract]. Fed Proc. 1985;44:592. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Cape Fear Valley Medical Center Fayetteville, NC 28302 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 January 1986Volume 104, Issue 1Page: 127-127KeywordsArthritisBloodFelty syndromeLymphocytesNeutropeniaRheumatoid arthritis Issue Published: 1 January 1986 PDF DownloadLoading ...
Human growth hormone prevents the protein catabolic side effects of prednisone in humans.
Authors: F F Horber, M W Haymond
Journal: Journal of Clinical Investigation (1990)

Prednisone treatment causes protein wasting and adds additional risks to a patient, whereas human growth hormone (hGH) treatment causes positive nitrogen balance. To determine whether concomitant administration of hGH prevents the protein catabolic effects of prednisone, four groups of eight healthy volunteers each were studied using isotope dilution and nitrogen balance techniques after 7 d of placebo, hGH alone (0.1 mg.kg-1.d-1), prednisone alone (0.8 mg.kg-1.d-1), or prednisone plus hGH (n = 8 in each group). Whether protein balance was calculated from the leucine kinetic data or nitrogen balance values, prednisone alone induced protein wasting (P less than 0.001), whereas hGH alone resulted in positive (P less than 0.001) protein balance, when compared to the placebo-treated subjects. When hGH was added to prednisone therapy, the glucocorticoid-induced protein catabolism was prevented. Using leucine kinetic data, negative protein balance during prednisone was due to increased (P less than 0.05) proteolysis, whereas hGH had no effect on proteolysis and increased (P less than 0.01) whole body protein synthesis. During combined treatment, estimates of proteolysis and protein synthesis were similar to those observed in the placebo treated control group. In conclusion, human growth hormone may have a distinct role in preventing the protein losses associated with the administration of pharmacologic doses of glucocorticosteroids in humans.
Cardiovascular Problems in Patients on Long-Term Hemodialysis
Authors: John P. Merrill
Journal: JAMA (1974)

Singh and his colleagues have recorded in this issue their experience with cardiac tamponade in chronic renal failure (p 1132). They conclude that elective partial pericardiectomy is the treatment of choice in all patients with uremic cardiac tamponade, thus confirming the observations and conclusions made by Ghavamian and his colleagues 1 in an identical patient population.  The problem of uremic pericardial tamponade is a classic example of the confusion that exists in evaluation of the efficacy of dialysis in the treatment of the cardiovascular complications of chronic renal failure by dialysis. Nowhere is this confusion better exemplified than in the problem of pericardial effusion and tamponade. Excellent observers, with thousands of patient-hours to their credit, disagree on (1) whether or not adequate dialysis prevents pericardial effusion and tamponade 2-5 and (2) whether pericardiocentesis alone is adequate 2,6,7 or whether pericardiectomy is necessary. 1,4 Singh and his colleagues point out that
Neurobehavioral Effects of Anticonvulsants
Authors: Michael R. Trimble
Journal: JAMA (1991)

A controversial issue in epilepsy research involves the effects of anticonvulsant drugs on cognitive function and behavior. The article by Dikmen and colleagues<sup>1</sup>in this issue ofThe Journalis an important contribution to the subject, especially as it addresses the issue in nonepileptic subjects. Thus, one problem of much work in this area relates to experimental design when the illness in question contaminates the results of any study. Seizures thus impair cognition, and anticonvulsant drugs, by changing seizure frequency, can render interpretation of data difficult. There are several ways to tackle this; one relates to using nonepileptic populations. An earlier attempt in this direction was made by Thompson and colleagues.<sup>2</sup>They used healthy volunteers who were prescribed anticonvulsant drugs in clinically equivalent doses for 14 days, testing cognitive function in a double-blind crossover study against placebo. Phenytoin significantly impaired performance on a variety of tasks, and in
THE EFFECT OF FIFA 11+ AS AN INJURY PREVENTION PROGRAM IN YOUTH FUTSAL PLAYERS
Authors: MI Zein, J Kurniarobbi, N Agung
Journal: British Journal of Sports Medicine (2014)

Background Futsal is a high risk sport, especially in youth level. But none has studied the application of FIFA 11+ , the football injury prevention program among youth futsal players in Indonesia. Objective To determine the effectiveness of FIFA 11+ in improving physical fitness components affecting injury risks among youth futsal players. Design This experimental study was recruiting two groups of youth futsal players by doing purposive/judgmental random sampling to the senior high school futsal teams in Yogyakarta, Indonesia. Setting High school futsal league. Participants The subjects were high school students participating in the school futsal team. From the total of 28 subjects (15 subjects in the experiment group and 13 in control group), 8 subjects were dropping out, leaving 9 subjects in the experiment group and 11 in the control group for the final analysis. Risk factor assessment The experiment (EXP) group underwent FIFA 11+ training twice per week for four weeks while the control (CON) group underwent routine futsal training. Both groups performed physical fitness tests before and after the intervention. Changes in performance (pre- vs. post-intervention) for each group were analyzed using dependent t-test (P&lt;.05). Change in performance of each group were compared using independent t-test (P&lt;.05). Main outcome measurements Core strength (evaluated using plank test), leg power (vertical jump test) and agility (Illinois agility test). Results: The core strength (P=.007) and agility (P=.01) of the EXP group increased significantly, while no change were observed in the CON group. The increase of agility in EXP group was found to be significantly different compared to the one in CON group (P=.039). Conclusions: FIFA 11+ can improve certain physical fitness components that contribute in preventing injury. Thus it is recommended to add FIFA 11+ to the routine futsal training as an injury prevention program Table 1 Pre-post intervention result. .
Dietary Estrogens Act through Estrogen Receptor-Mediated Processes and Show No Antiestrogenicity in Cultured Breast Cancer Cells.
Authors: S Makela, VL Davis, WC Tally, J Korkman, L Salo, R Vihko, R Santti, KS Korach
Journal: Environmental Health Perspectives (1994)

Dietary estrogens are believed to exert their estrogenic or antiestrogenic (chemopreventive) action in estrogen responsive cells by interacting with the estrogen receptor (ER). The present study was undertaken to evaluate a direct role of ER in estrogenic or antiestrogenic activities of three dietary estrogens (coumestrol, genistein and zearalenone). HeLa cells were transiently co-transfected with an expression vector for ER and an estrogen-responsive reporter gene construct. Coumestrol, genistein, and zearalenone all increased the activity of the reporter gene, only in the presence of the ER, and the activation was blocked with the ER antagonist ICI 164,384, demonstrating an ER-specific, agonist response. In addition, in MCF-7 cells, coumestrol and zearalenone increased the expression of the estrogen-responsive pS2 gene. Coumestrol and genistein inhibited the purified estrogen-specific 17ß-hydroxysteroid oxidoreductase enzyme and the conversion of estrone to 17ß-estradiol in T-47D cells, which contain this enzyme. However, they did not inhibit the estrone-induced proliferation of T-47D cells. In conclusion, coumestrol, genistein, and zearalenone are all potent estrogens in vitro, and they act through ER mediated mechanism. Our findings give no evidence to support the idea that these compounds act as antiestrogens through competition for the binding sites of ER or by inhibition of the conversion of estrone to 17ß-estradiol in breast cancer cells, since this effect was nullified by their agonist action on cell proliferation. Therefore, their suggested chemopreventive action in estrogen-related cancers must be mediated through other mechanisms.
Linear melanonychia due to subungual keratosis of the nail bed: a report of two cases
Authors: None BARAN, None PERRIN
Journal: British Journal of Dermatology (1999)

Longitudinal melanonychia displaying features of keratinized acanthoma is described in two patients. In both cases, a pigmented band consisting of a subungual keratinized epithelial ridge originated in the nail bed. We have been able to show, using the clinical and histological findings, that the origin of the pigment is linked to its synthesis within the acanthoma of the nail bed. These lesions are reminiscent of pigmented seborrhoeic keratosis. The horny cysts are replaced, because of the special physiological longitudinal arrangement of the ridges in the nail bed, by a single, prominent, longitudinal, keratinized and pigmented mass.
Plasmapheresis in the initial treatment of insulin-dependent diabetes mellitus in children.
Authors: J Ludvigsson, L Heding, G Lieden, B Marner, A Lernmark
Journal: BMJ (1983)

Several factors indicate that autoimmune mechanisms may play a part in the aetiology of insulin-dependent diabetes mellitus. At the onset of the disease in 10 children (aged 11-16 years) plasmapheresis was performed four times over one to two weeks. Seventeen age-matched children with the same clinical features served as controls. The C-peptide concentrations at onset were the same in the two groups, but after one month the children treated with plasmapheresis had significantly higher values. This difference became even more pronounced after three, nine, and 18 months, both during fasting and at the maximum response to a standardised meal. The study group also had a significantly more stable metabolism, longer partial remission, and no higher insulin requirement. Of the 10 treated children islet-cell cytoplasmic antibodies were present in seven before plasmapheresis and in nine during treatment. The antibodies remained detectable in five and six out of nine patients at one and six months respectively after plasmapheresis. Although the mechanisms are obscure, plasmapheresis performed at the onset of insulin-dependent diabetes mellitus may help to preserve beta-cell function.
Future Medical Practice in the United States
Authors: John P. Geyman
Journal: JAMA (1981)

IN 1966, Creech<sup>1</sup>published a provocative article entitled "Medical Practice in 1990," wherein he projected the likely features of the medical care system based on apparent trends in medical education and practice since 1890 in this country. He painted a bleak scenario for 1990, illustrated by the following excerpts: The private practice of medicine will no longer exist as we know it. Physicians will be geographic full-time employees of the medical center complex, within which they will provide total medical care for the residents of the community, preferably on an annual fee basis, but perhaps as salaried employees of the federal government.... Medicine will be practiced on an assembly line basis.... [Physicians] will no longer concern themselves with the routine practice of medicine, which will be done by others whose training is more vocationally oriented. In just 14 years since 1966, major changes have taken place in medical education
286 Dendritic cells in mononuclear cells from peripheral blood in patients with autoimmunological liver diseases (PBC, AIH) and alcoholic liver disease (ALD)
Authors: J. Daniluk, A. Zwolak, B. Kasztelan-Szczerbinska, J. Rolinski, A. Surdacka, C.T. Lozowski
Journal: Journal of Hepatology (2006)
Israel's health maintenance organisations will have to publish their performance data
Authors: J. Siegel-Itzkovich
Journal: BMJ (2011)

After four years of litigation, an Israeli freedom of information group has won a battle to make the health ministry provide data on the performance of the four public health maintenance organisations. 

The group, called the Movement for the Freedom of Information, was victorious in the Jerusalem district court, which ruled that starting in April 2012 the ministry must publish comparative data on as many as 50 health indicators.

Until now the ministry has said that the “health funds,” which both insure all 7.5 million Israelis and provide medical services, could release …
Quebec doctors must tell patients about medical "accidents"
Authors: D. Spurgeon
Journal: BMJ (2002)

A new ethics code announced this month by the Quebec College of Physicians states that doctors in Quebec must tell their patients about all “incidents,” “accidents,” or “complications” that occur during treatment and that could have a substantial impact on the patient's health. Doctors who do not reveal such events to their patients could be subject to fines or suspension. But the code does not use the term “medical errors.”

“When one speaks of errors there is an implication of …
Inhibition of Invasion of Invasive Human Bladder Carcinoma Cells by Protein Kinase C Inhibitor Staurosporine
Authors: Gary K. Schwartz, S. Mark Redwood, Takao Ohnuma, James F. Holland, Michael J. Droller, Brian C.–S. Liu
Journal: JNCI Journal of the National Cancer Institute (1990)

To study the effect of the protein kinase C (PKC) inhibitor staurosporine on invasion, we selected the invasive human bladder carcinoma cell line EJ. Total PKC activity was more than twofold higher in the EJ cells than in RT4 cells (superficial human bladder carcinoma cells), which do not pass through an artificial basement membrane. There was more PKC activity in the cytosol than in the membrane of EJ cells. Staurosporine, at nontoxic concentrations, inhibited the invasion of EJ cells through an artificial basement membrane in a dose-dependent manner. Staurosporine caused a dose-dependent inhibition of cell motility but did not inhibit cell attachment. Staurosporine represents a new agent for the inhibition of tumor cell invasion and may prove useful in studying the mechanisms responsible for this phenomenon.