tbuckley/cabot-search huggingface dataset:
Abstract 3825: Inhibitory effect of PID1 in medulloblastomas, gliomas and ATRT.
Authors: Anat Erdreich-Epstein, Xiuhai Ren, Hong Zhou, Richard Sposto, Lingyun Ji, Stefan Pfister, Marcel Kool, Shahab Asgharzadeh
Journal: Cancer Research (2013)

Abstract The recently identified PID1 (Phosphotyrosine Interaction Domain containing 1) is a modulator of insulin signaling in adipocytes and muscle cells. Using a number of independent datasets we show that mean PID1 mRNA was lower in unfavorable medulloblastomas (subgroups 3 and 4) and in glioblastomas compared with favorable medulloblastomas (SHH and WNT subgroups) or normal brain, respectively. Higher PID1 mRNA correlated with longer progression-free survival and overall survival in medulloblastoma patients and with longer overall survival in glioma patients. Ectopic expression of PID1 in medulloblastoma, glioblastoma and ATRT (atypical teratoid rhabdoid tumor) cell lines inhibited colony formation by 68% ± 3.5 (p&amp;lt;0.0001 in total of 24 experiments in triplicates) compared with controls. PID1 effects on apoptosis and proliferation were demonstrated by increased sub-G0/G1 and annexinV staining, decreased S-phase. Additionally, PID1 induced mitochondrial depolarization in the medulloblastoma, glioblastoma, and ATRT cell lines. In keeping with PID1 having a PTB (phosphotyrosine binding) domain, endogenous PID1 co-immunoprecipitated with the low density lipoprotein receptor-related protein-1 (LRP1, which is a potential binding partner for PID1 via its C-terminal NPXY motif). However, PID1 with mutated PTB domain retained its inhibitory effects on colony formation, suggesting that other domains of PID1 mediated (or contributed to) PID1’s inhibitory effect. Analysis of PID1 domains comprising its N-terminal region (D1), C-terminal region (D3) and middle region (D2), each comprising approximately 1/3 of the protein, revealed that indeed, each 1/3 of PID1 inhibited colony formation at least as efficiently as full length PID1. Interestingly, although by itself D2 was more inhibitory than full length PID1, when it was combined with D3, which was also inhibitory by itself, we observed marked mitigation of the inhibitory effect of the combined D2+D5 fragment on colony formation, suggesting functional intramolecular interactions in PID1. These data suggest for the first time that PID1 has a tumor suppressor-like effect in brain tumors and shed light on the molecular mechanism of its inhibitory action. Citation Format: Anat Erdreich-Epstein, Xiuhai Ren, Hong Zhou, Richard Sposto, Lingyun Ji, Stefan Pfister, Marcel Kool, Shahab Asgharzadeh. Inhibitory effect of PID1 in medulloblastomas, gliomas and ATRT. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3825. doi:10.1158/1538-7445.AM2013-3825
Gene Therapy Using Adenovirus-Mediated Full-length Anti-HER-2 Antibody for HER-2 Overexpression Cancers
Authors: Minghong Jiang, Wenfang Shi, Qi Zhang, Xinhua Wang, Minggao Guo, Zhenfu Cui, Changqin Su, Qing Yang, Yuemin Li, Jonathan Sham, Xinyuan Liu, Mengchao Wu, Qijun Qian
Journal: Clinical Cancer Research (2006)

Abstract Purpose: Therapeutic monoclonal antibody is increasingly applied in many clinical applications, although complicated technologies and high cost still limit their wide applications. To obtain the sustained serum antibody concentration with one single injection and lower the cost of antibody protein therapy, an adenovirus-mediated full-length antibody gene therapy was developed. Experimental Design: Full-length antibody light-chain and heavy-chain sequences were linked with internal ribosome entry site and constructed into adenoviral vector under the control of cytomegalovirus promoter. Antibody expression in vitro and in vivo were tested with ELISA, and its antitumor efficacy was evaluated in SKOV-3-inoculated nude mice. Results: Ad5-TAb–generated anti-HER-2 antibody presented the similar binding specificity with commercial trastuzumab. A single i.v. injection of 2 × 109 plaque-forming units of Ad5-TAb per mouse resulted in not only a sustained over 40 μg/mL serum antibody level for at least 4 weeks but also significant tumor elimination in the ovarian cancer SKOV-3-inoculated nude mice. Conclusions: An in vivo full-length antibody gene delivery system allows continuous production of a full-length antibody at high concentration after a single administration. Bioactive antibody macromolecules can be generated via gene transfer in vivo. All the data suggest that this novel adenovirus-mediated antibody gene delivery can be used for the exploitation of antibodies, without being hampered by the sophisticated antibody manufacture techniques and high cost, and, furthermore, can shorten the duration and reduce the expense of antibody developments.
Thirst Fever, with a Characteristic Temperature Pattern in Infants in Kuwait
Authors: Y. Shaker
Journal: BMJ (1966)

Oral Contraceptives-Thulin and Nermark JOU?4 LC performed in six of the cases with histological changes very similar to those seen in chlorpromazine-induced jaundice.The laboratory findings were a normal or only slightly raised alkaline phosphatase value and high transaminase levels.Certain features in the history were present in several cases: two had suffered from icterus and pruritus gravidarum, and this should be regarded as a contraindication to the use of contraceptive pills.The widespread use of oral contraceptives leads us to the supposition that this cause for jaundice is sometimes overlooked.
The Hazards of Fallout or of Epidemiologic Research?
Authors: Charles E. Land
Journal: New England Journal of Medicine (1979)

A paradox that is likely to remain with us for some time is that although we probably know more about the carcinogenic effects of ionizing radiation than about those of any other environmental agent, our knowledge about radiation effects at low-dose levels is inadequate. Risk estimates now available depend heavily on low-dose extrapolation of observed cancer rates corresponding to higher-dose levels. In this issue of the Journal, Lyon et al. report finding increased leukemia mortality among children in areas of Utah exposed to radioactive fallout from the atmospheric tests of nuclear weapons carried out in Nevada from 1951 through . . .
Anemia in Erythropoietic Protoporphyria
Authors: Micheline M. Mathews-Roth
Journal: JAMA (1974)

<h3>To the Editor.—</h3> During the course of our recently reported study on β-carotene treatment of erythropoietic protoporphyria (EPP) (228:1004, 1974), we found that some patients had some degree of anemia as defined by the criteria established by the AMA Council on Foods and Nutrition (203:407, 1968). Since EPP was thought to have no hematological abnormality,<sup>1,2</sup>we present our findings to suggest that a mild degree of anemia may be more widespread among patients with EPP than had been thought. The Table shows the incidence of anemia in our patients. Calculation of red blood cell indices in 12 of the 19 patients classified as anemic showed that the mean corpuscular volume was decreased in seven patients, and the mean corpuscular hemoglobin count was within the lower half of the normal range of 32 to 36 (31 to 33); this would suggest the presence of a microcytic-normochronic anemia. Examination of blood
Doctor sentenced for manslaughter of leukaemia patient
Authors: C. Dyer
Journal: BMJ (2003)

A specialist registrar who supervised the mistaken injection of a cytotoxic drug into the spine of a teenager with leukaemia was sentenced to eight months in prison on Tuesday after pleading guilty to manslaughter.

Feda Mulhem changed his plea as a retrial was about to begin at Nottingham Crown Court. An earlier trial had to be abandoned because of his ill health ( BMJ 2003;327: 123). …
CHANGES IN BACTERIAL VOLUME AS THE RESULT OF SPECIFIC AGGLUTINATION
Authors: F. S. Jones, Ralph B. Little
Journal: The Journal of Experimental Medicine (1933)

Measurements indicate that bacterial antigens increase in volume as the result of specific agglutination. There is a general parallelism between the increase in antigenic volume and the concentration of the immune serum. The phenomenon is specific. There is no increase with normal serum; with absorbed serum the increase is slight and it can be correlated with the presence of unabsorbed antibody. The effect is enduring as shown by volumetric determinations upon repeatedly washed, agglutinated bacteria.
How can selection-for-perception be decoupled from selection-for-action?
Authors: Cécile Beauvillain, Pierre Pouget
Journal: Behavioral and Brain Sciences (2003)

Evidence is presented for the notion that selection-for-perception and selection-for-action progress in parallel to become tightly coupled at the saccade target before the execution of the movement. Such a conception might be incorporated in the E-Z Reader model of eye-movement control in reading.
Government responds to Stafford inquiry with new "whistleblower in chief" to rate hospitals
Authors: C. Dyer
Journal: BMJ (2013)

NHS hospital trusts in England will be placed under a statutory duty of candour to be open with patients and regulators if they believe that treatment or care has caused death or serious injuries, and organisations that flout the duty will incur criminal penalties.

The measure is part of a package of reforms aimed at creating a new culture of openness and safety in the NHS, after widespread institutional failings were laid bare by the public inquiry into events at Mid Staffordshire NHS Foundation Trust between 2005 and 2009. 

The public inquiry, which reported in February, painted a damning picture of a top-down culture in which patients were subjected to “appalling” care in the pursuit of management targets, while various regulators failed to pick up that the system was failing.1

Robert Francis QC, who chaired the inquiry, recommended that it should be made a criminal offence for any doctor, nurse, or director of a healthcare organisation to provide intentionally misleading information to …
CTLA-4 dysregulation of self/tumor-reactive CD8+ T-cell function is CD4+ T-cell dependent
Authors: Luca Gattinoni, Anju Ranganathan, Deborah R. Surman, Douglas C. Palmer, Paul A. Antony, Marc R. Theoret, David M. Heimann, Steven A. Rosenberg, Nicholas P. Restifo
Journal: Blood (2006)
Growth, development, and reassessment of hypothyroid infants diagnosed by screening.
Authors: J A Hulse, D B Grant, D Jackson, B E Clayton
Journal: BMJ (1982)

Thirty]six neonates in whom hypothyroidism was diagnosed after thyroid stimulating hormone screening were reassessed at 1 year. All had grown satisfactorily and the mental development scores were normal in all except two. Treatment was withdrawn in 32 and persistent hypothyroidism was confirmed in 31 cases. Thyroid stimulating hormone concentrations were raised in one-third of cases before the withdrawal of treatment and this was associated with generally lower concentrations of serum thyroxine (T4) and smaller doses of L-thyroxine than in those cases with normal concentrations of thyroid stimulating hormone. In treating congenital hypothyroidism, serum T4 concentrations should be monitored regularly and the dose of thyroxine adjusted to maintain serum T4 in the upper part of the reference range.
Repeat Dilation of Intravascular Stents in Congenital Heart Defects
Authors: Frank F. Ing, Ronald G. Grifka, Michael R. Nihill, Charles E. Mullins
Journal: Circulation (1995)

Intravascular (Palmaz) stents have been successfully implanted in patients with congenital and acquired branch pulmonary stenosis. Early results are excellent; however, there is no information on restenosis and repeat dilation in patients with congenital heart disease. The purpose of this study is to review the incidence of restenosis and demonstrate the safety and efficacy of repeat dilation of stents in this group of patients.Of 94 patients with 163 implanted stents in this single-center study, 43 patients with 73 implanted stents underwent recatheterization. Only 2 of 73 restudied stents (3%) developed significant restenosis. In 20 patients, 30 stents were redilated. At stent implantation, the mean age of this subgroup was 14.2 years, the mean intraluminal diameter increased from 4.9 to 10.7 mm (P = .0001), and the systolic gradient (mean) across the stent decreased from 52 to 11 mm Hg (P = .0001). At recatheterization (mean, 13 months), all stents were patent. The mean diameter decreased by 1.2 mm (P = .0001), but the increase in the gradient (mean, 3 mm Hg) was not significant (P = .11). After repeat dilation, the diameter increased from 9.5 to 12.2 mm (P = .0001), and the gradient decreased from 14 to 8 mm Hg (P = .0003). The 2 stents with restenosis were redilated successfully. Two patients underwent a successful second redilation of 3 stents at 18 and 26 months. There were no complications.All stents remained patent. The occurrence of significant restenosis is low (3%), and these restenoses can be redilated and/or restented. Repeat dilation of the Palmaz stent implanted in branch pulmonary artery stenosis can be performed with safety and efficacy (94% success rate) up to 3 years after stent implantation.
Reply to David R. Staskin and Michael G. Oefelein's Letter to the Editor re: Giacomo Novara, Antonio Galfano, Silvia Secco, et al. A Systematic Review and Meta-Analysis of Randomized Controlled Trials with Antimuscarinic Drugs for Overactive bladder. Eur Urol 2008;54:740–64
Authors: Giacomo Novara, Walter Artibani
Journal: European Urology (2008)
Abstract 14874: Low Cardiorespiratory Fitness is Associated with Smaller Heart Size, Increased Concentricity and Worsening Diastolic Function: The Cooper Center Echocardiographic Sub-Cohort
Authors: Stephanie K Brinker, Colby R Ayers, Carolyn E Barlow, Laura F DeFina, Benjamin L Willis, Nina B Radford, Ramin Farzaneh-Far, James A de Lemos, Mark H Drazner, Jarett D Berry
Journal: Circulation (2012)

Background: Higher physical activity is inversely associated with heart failure risk. However, the mechanism by which exercise lowers this risk is not fully understood. We sought to determine the c...
345. Gene Therapy for Wiskott-Aldrich Syndrome Using Lentiviral Vectors: Evidence for Efficacy and Safety after Transduction of Human T Cells and Hematopoietic Stem Cells
Authors: Loïc Dupré, Samantha Scaramuzza, Francesco Marangoni, Sara Trifari, Silvana Martino, Shigeru Tsuchiya, Adrian Thrasher, Anne Galy, Luigi Naldini, Alessandro Aiuti, Maria Grazia Roncarolo
Journal: Molecular Therapy (2005)

Wiskott-Aldrich syndrome (WAS) is a X-linked primary immunodeficiency with a median survival of 14 years of age due to infections, severe hemorrhage, and lymphomas. Transplantation of hematopoietic stem cells (HSC) from HLA-identical sibling donors is a resolutive treatment, but is available for a minority of patients. Transplantation of genetically corrected autologous HSC could represent an alternative treatment applicable to all patients. We investigated the efficacy and safety of WAS gene transfer with HIV-based lentiviral vectors using untransformed T cells from WAS patients and HSC.
MEPROBAMATE AND SMALL AMOUNTS OF ALCOHOL
Authors: George A. Zirkle
Journal: JAMA (1960)

This study was designed to determine whether a combination of meprobamate and alcohol interferes with human ability, coordination, and judgment more than either of these drugs taken singly. Eight psychological tests were used to measure the effects of the drugs, which were administered blind to 22 normal subjects. Sufficient alcohol was used to produce a concentration of 0.05 % in the blood. Meprobamate dosage was 400 mg. four times daily for a week before the test. Four rotated drug conditions were provided: (1) placebo tablet and placebo drink, (2) meprobamate tablet and placebo drink, (3) alcohol drink and placebo tablet, and (4) meprobamate tablet and alcohol drink. Over-all test performance was best in condition (1) and worst in (4). It is recommended that physicians who prescribe meprobamate warn their patients of the increased danger of consuming alcoholic beverages.
Crohn's Disease in the Esophagus - Report of a Case
Authors: H. Kuboi, K. Yashiro, H. Shindou, H. Sasaki, N. Hayashi, K. Nagasako
Journal: Endoscopy (1988)

A case of esophageal Crohn's disease is described. Crohn's lesion was observed in the lower esophagus, and sarcoid-like granulomas were found in the biopsied specimen. A clinical consideration of this phenomenon is presented.
Morphine Receptor Cloned—Improved Analgesics, Addiction Therapy Expected
Authors: Teri Randall
Journal: JAMA (1993)

ENDING A QUEST that has engaged some of the most productive minds in pain research for two decades, researchers last month unveiled the genetic sequence of the molecular receptor for morphine. With the sequence now published, experts are predicting a burgeoning of knowledge about the molecular mechanisms of pain and its concomitant mysteries of analgesia, tolerance, and addiction. It is also hoped that the discovery will facilitate the development and testing of new pain relievers that are more potent than morphine and have fewer undesirable effects. Of the three major classes of opioid receptors—μ, δ, and κ—it is the μ receptor, named for morphine's initial letter, that has drawn the most attention from scientists. It is through this receptor on the surface of nerve cells, this gateway, that the body experiences the analgesic and euphoric effects of almighty morphine. Although the μ receptor was first identified in the 1970s, no
Spilker's Guide to Clinical Trials on CD-ROM
Authors: Charles Weijer
Journal: JAMA (1997)

Clinical Trials Spilker's Guide to Clinical Trials on CD-ROM includes two of Spilker's books,<i>Guide to Clinical Trials</i>(1991) and<i>Patient Recruitment in Clinical Trials</i>(1992), plus 11 chapters of previously unpublished material. In total, the volume includes 157 chapters on practical aspects of the planning and conduct of clinical trials, particularly pharmaceutical industry trials. The bulk of the material included in the CD-ROM has already been reviewed in the pages of<i>JAMA</i>.<sup>1</sup>The reviewer concluded that Spilker's work was "... unique in its scope and breadth... an essential component of the library of anyone involved in conducting clinical trials." Given this finding, I will restrict my review to the question, should one buy the CD-ROM or the printed book? The CD is compatible with both PC and Macintosh systems—PCs must have at least a 486 processor, 2MB RAM, and 5 MB free space on the hard drive; Macs must
Antimicrobial Selection by a Computer
Authors: Victor L. Yu
Journal: JAMA (1979)

An evaluation of a computer-based consultation system called MYCIN was made. Eight independent evaluators with special expertise in the management of meningitis compared MYCIN's choice of antimicrobials with the choices of nine human prescribers for ten test cases of meningitis. MYCIN received an acceptability rating of 65% by the evaluators; the corresponding ratings for acceptability of the regimen prescribed by the five faculty specialists ranged from 42.5% to 62.5%. The system never failed to cover a treatable pathogen while demonstrating efficiency in minimizing the number of antimicrobials prescribed. The study design may be useful in assessing the performance of other computer-based clinical decision-making systems. (<i>JAMA</i>242:1279-1282, 1979)
Cells expressing a major histocompatibility complex class I molecule with a single covalently bound peptide are highly immunogenic.
Authors: E Mottez, P Langlade-Demoyen, H Gournier, F Martinon, J Maryanski, P Kourilsky, J P Abastado
Journal: The Journal of Experimental Medicine (1995)

The major histocompatibility complex (MHC) class I molecules expressed at the cell surface are associated with a large number of different peptides so that the density of a given MHC-peptide complex is relatively low. Here we describe the properties of MHC class I molecules genetically attached to a single antigenic peptide. Cells expressing these fusion proteins are recognized by T cells specific for the particular MHC-peptide complex. Coculture of naive splenocytes with cells expressing these MHC-peptide fusion proteins and the B7.1 antigen allows the induction of primary cytotoxic T lymphocytes (CTL) in vitro. Injection of these cells into naive mice enhances the frequency of specific CTL precursors and protects against a subsequent challenge with a tumor or a virus bearing the antigenic peptide. Soluble MHC-peptide fusions were also produced in which all three components, that is, the heavy chain, beta 2-microglobulin and the peptide, have fused into a single-chain protein. The availability of MHC class I molecules bound to a single peptide provides valuable tools for the manipulation of CTL responses and the analysis of the selection processes in the thymus.
The Cost-Coverage Trade-off
Authors: Ezekiel J. Emanuel
Journal: JAMA (2008)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
UK audit will review pathologists' reports on adult autopsies
Authors: Susan Mayor
Journal: BMJ (2004)

The National Confidential Enquiry into Patient Outcome and Death (NCEPOD)—an independent UK body that does audits designed to assess and improve patient care—announced plans last week to review reporting procedures for adult autopsies ordered by coroners.

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An autopsy in progress 

Credit: MEDISCAN

The study will assess the quality of pathologists' reports on adult autopsies requested by coroners, which account for 90% of postmortem examinations in the United Kingdom. It will …

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Stuck in the middle?
Authors: D. Lock
Journal: BMJ (2001)

I have just emerged from three years stuck between the medical profession and NHS managers on one side and the public, the media, and local politicians on the other. The only issue was whether or not the NHS could continue to run a blue light accident and emergency department at Kidderminster Hospital, one of the smallest district general hospitals in the country, with no paediatric department and just a single consultant in emergency medicine.

> Outwardly popular but dangerous services simply cannot be allowed to continue 

I was the local member of parliament, a government minister, and the husband of a general practitioner. What could an MP do in these circumstances? Well there were only two choices—either back the changes recommended by professionals or back a futile, misguided but popular (and populist) campaign against them. No MP could try to sit in the middle of this storm.

Arguments for scaling down the emergency department to a minor injuries unit were overwhelming. The local consultants said that the department wasn't safe and the King's Fund, brought in …