tbuckley/cabot-search huggingface dataset:
PCR‐based cloning, isolation, and IgE‐binding properties of recombinant latex profilin (rHev b 8)
Authors: H‐P. Rihs, Z. Chen, P. Rozynek, X. Baur, M. Lundberg, R. Cremer
Journal: Allergy (2000)

Profilin (Hev b 8) in natural rubber latex (NRL) has been assumed to be an important allergen. Since latex profilin has a molecular mass similar to two other latex allergens (Hev b 1 and Hev b 6.03) in the 14-kDa range, it is difficult to obtain sufficient amounts of purified native profilin for investigations and diagnostics. The present study aimed to produce recombinant latex profilin (rHev b 8) and study its IgE-binding reactivity.A profilin-specific cDNA encoding the latex profilin from Hevea brasiliensis leaves was synthesized and subcloned, and the rHev b 8 was overexpressed in fusion with the maltose-binding protein (MBP) in E. coli. The IgE-binding reactivity of rHev b 8 was studied by immunoblotting, immunoblot inhibition experiments, and the Pharmacia CAP method, with 25 sera from health-care workers with latex allergy and 17 sera from latex-sensitive spina bifida patients.rHev b 8 was found to have 131 amino acids and a sequence identity of 75% with birch profilin (Bet v 2). Analysis by the CAP system revealed the presence of rHev b 8-specific IgE antibodies in two out of 17 sera from spina bifida patients and in five out of 25 sera (20%) from health-care workers. Two subjects of the latter group with rHev b 8-specific IgE showed negative results in the skin prick tests with tree-pollen extracts and had no IgE to rBet v 2, indicating the presence of IgE-binding epitopes on the Hev b 8-molecule which do not cross-react with birch profilin. Immunoblot inhibition assays using MBP-rHev b 8 as inhibitor confirmed the presence of latex profilin in the NRL extract. IgE binding to the native latex profilin could be completely inhibited by the MBP-rHev b 8.Latex profilin represents a minor allergen in NRL and may have IgE-binding epitopes different from Bet v 2.
Fusobacterium necrophorum Septicemia
Authors: James Adams
Journal: JAMA (1983)

<h3>To the Editor.—</h3> We wish to add to the list of serious complications associated with<i>Fusobacterium necrophorum</i>septicemia as reported by Seidenfeld, Sutker, and Luby (1982; 248:11:1348). <h3>Report of a Case.—</h3> A 16-year-old girl was admitted to the hospital was suspected meningitis. Five weeks earlier, she had been seen when results from a slide test for infectious mononucleosis heterophil antibodies were positive. One week before admission, she experienced an upper respiratory tract infection. Two days before admission, she noted thick nasal discharge and a severe, unrelenting headache. At the time of admission, she had moderate nuchal rigidity and a WBC count of 23,600/cu mm. Admission spinal tap and CSF examination findings were unremarkable. Because of increasing nuchal rigidity, a syncopal spell, and a new left-sided Horner's syndrome, the spinal tap was repeated two days later, revealing an elevated CSF WBC count of 830/cu mm, 73% polymorphonuclear leukocytes, and 27% monocytes.
Immunologic hierarchy and promiscuity of melanoma helper peptides
Authors: Yinin Hu, Gina R Petroni, Walter C Olson, Andrea Czarkowski, Mark Smolkin, William W Grosh, Patrice K Rehm, Cheryl F Murphy, Elizabeth J Coleman
Journal: Journal for ImmunoTherapy of Cancer (2013)

Meeting abstracts Melanoma vaccines have been designed to expand specific CD8+ T-cells, but melanoma-reactive helper T-cells also can have antitumor activity. We previously observed clinical activity of a vaccine incorporating 6 melanoma helper peptides (6MHP), and found associations between CD4+ T
Death of Marat I: Artsy Smut? Plain Brown Wrapper?
Authors: Linda Gragg Smith
Journal: JAMA (1993)

To the Editor.  —I am not a physician, though I did work as an administrative assistant for risk management at University of Washington Hospital while my husband attended medical school; he is the physician in the house. His JAMA is delivered to our home where we live with our two young (aged 6 and 3 years) children. Perhaps most physicians' wives screen their husbands' mail more thoroughly than I do. Ours is usually dumped all together in a chair at the top of the stairs and then sorted through sooner or later.  Mine was not a knee-jerk reaction on first seeing the cover of the June 10, 1992, issue. 1 But it perturbed me when I imagined how traumatic an image it could have been for my children had they chanced to see it first.  Our family enjoys having JAMA delivered to our home; the kids and I especially so
Valvular Dysfunction and Carotid, Subclavian, and Coronary Artery Disease in Survivors of Hodgkin Lymphoma Treated With Radiation Therapy
Authors: Matthew C. Hull
Journal: JAMA (2003)

The majority of patients with Hodgkin lymphoma are young and highly curable. This necessitates concern for prevention, diagnosis, and optimal management of potential treatment-related complications.To identify and quantify the incidence of and factors contributing to long-term cardiac and vascular complications after radiation therapy for Hodgkin lymphoma.Retrospective study comparing patients treated from 1962 to 1998 at a university-based referral center with a matched general population.Four hundred fifteen consecutive patients who fulfilled the inclusion criteria of a minimum 2-year follow-up (median, 11.2 years) and whose radiation fields included the heart or carotid or subclavian arteries.Multivariable analyses of potential risk factors and observed-to-expected ratios for cardiac valve surgery, coronary artery bypass graft surgery, percutaneous coronary intervention, or both based on Surveillance, Epidemiology, and End Results (SEER) and National Hospital Discharge Survey (NHDS) data.Forty-two patients (10.4%) developed coronary artery disease at a median of 9 years after treatment, 30 patients (7.4%) developed carotid and/or subclavian artery disease at a median of 17 years after treatment, and 25 patients (6.2%) developed clinically significant valvular dysfunction at a median of 22 years. The most common valve lesion was aortic stenosis, which occurred in 14 valves. The observed-to-expected ratio for valve surgery was 8.42 (95% confidence interval [CI], 3.20-13.65) and the observed-to-expected ratio for coronary artery bypass graft surgery or percutaneous coronary intervention was 1.63 (95% CI, 0.98-2.28). At least 1 cardiac risk factor was present in all patients who developed coronary artery disease. The only treatment-related factor associated with the development of coronary artery disease was utilization of a radiation technique that resulted in a higher total dose to a portion of the heart (relative risk, 7.8; 95% CI, 1.1-53.2; P =.04). No specific treatment-related factor was associated with carotid or subclavian artery disease or valvular dysfunction. Freedom from any cardiovascular morbidity was 88% at 15 years and 84% at 20 years.Among patients treated with radiation therapy for Hodgkin lymphoma, there are statistically higher than expected rates of valve surgery and coronary revascularization procedures over the next 10 to 20 years. Coronary vascular disease is associated with higher radiation doses and traditional coronary heart disease risk factors. Noncoronary vascular disease and clinically important valvular dysfunction are less well understood complications at 15 to 20 years after radiation, requiring surveillance and further study.
Patch test results from the Mayo Clinic Contact Dermatitis Group, 1998-2000
Authors: David A. Wetter, Mark D.P. Davis, James A. Yiannias, Janet F. Cheng, Suzanne M. Connolly, Rokea A. el-Azhary, Sara A. Farmer, Debra D. Fett, Janis S. Johnson, Diane L. Nordberg Linehan, Donna M. Richardson, Arnold L. Schroeter
Journal: Journal of the American Academy of Dermatology (2005)
On the Good Effect of the Ergot of Rye in a Case of Retention of Urine
Authors: T. Jeffreys
Journal: BMJ (1844)

A KNIFE-BLADE. and speaking loudly into his ear, could be mnade to take medicine or thin fluid food.The pupil of the right eye was dilated to about double the size of the left, and I could not observe any contractile movement in it when the light was suddenly admitted.The left iris was in its normal state, though perhaps rather more
Lymphocyte Subpopulations in Sarcoidosis: Correlation with Disease Activity and Duration
Authors: RONALD P. DANIELE
Journal: Annals of Internal Medicine (1976)

We studied lymphocytes from the peripheral blood of 29 sarcoid patients to relate T- and B-cell populations with disease activity and duration. In patients with acute (< 1 year) and chronic (≥ 9 years) active disease, the absolute lymphocyte count was reduced; the absolute number of T cells was reduced; and the proliferative response of lymphocytes to phytohemagglutinin was depressed compared with control subjects. Ten of 21 patients with active disease had 5% to 32% atypical lymphocytes. The proportion of cells bearing surface immunoglobulin (Ig) was increased in patients with active disease, but the absolute number of cells bearing surface Ig did not differ significantly from controls. However, studies using overnight in-vitro culture indicated that a large fraction of these cells bound exogenous Ig. The number of cells identified by complement receptors was significantly reduced in patients with active disease. In contrast, patients who had complete resolution of their disease showed no significant differences from controls in either T- or B-cell populations or in the proliferative response of their lymphocytes to phytohemagglutinin.
If I'm in JAMA, Is This Heaven? Obituary Disputed
Authors: Dwight L. Evans
Journal: JAMA (1990)

To the Editor.—  Odd, that after returning from a marvelous talk reflecting on aging, life, and death, I should receive several remarkable telephone calls regarding my own death. It seems my obituary—name, age, schools, certifications, and the like—had been published in a medical journal.  Wanting to maintain a scientific posture (not a horizontal one), I asked: What were the circumstances? What are the competing hypotheses? Where was it published?  I soon left my meeting and returned home to the University of North Carolina at Chapel Hill. I researched the recently published finding and learned that the citation was JAMA . 1990;263: 1022; I had died 8 months earlier in June 1989. Yes? No?  Well, the scientific process and the report of scientific findings should be progressively self-correcting, approaching our best understanding regarding the truth of a given matter—so I always thought. Therefore, I offer the following:  It is well known that
A Medical Lesson
Authors: Carolyn Theresa Cleary
Journal: Annals of Internal Medicine (2000)

Ad Libitum16 May 2000A Medical LessonCarolyn Theresa Cleary, MDCarolyn Theresa Cleary, MDRochester, NY 14620 (Theresa)Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-132-10-200005160-00015 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail The words ... Author, Article, and Disclosure InformationAffiliations: Rochester, NY 14620 (Theresa)Corresponding Author: Carolyn T. Cleary, MD, Elmwood Pediatric Group, 125 Lattimore Road, Rochester, NY 14620. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 16 May 2000Volume 132, Issue 10Page: 840KeywordsHeartMouthReflexesStroke ePublished: 15 August 2000 Issue Published: 16 May 2000 Copyright & PermissionsCopyright © 2000 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Safety and Efficacy of Dacomitinib in Korean Patients with KRAS Wild-Type Advanced Non–Small-Cell Lung Cancer Refractory to Chemotherapy and Erlotinib or Gefitinib: A Phase I/II Trial
Authors: Keunchil Park, Byoung C. Cho, Dong-Wan Kim, Myung-Ju Ahn, Sang-Yoon Lee, Diana Gernhardt, Ian Taylor, Alicyn K. Campbell, Hui Zhang, Nagdeep Giri, Stephen P. Letrent, Joseph O’Connell, Dae S. Heo
Journal: Journal of Thoracic Oncology (2014)
The Physician Shortage, 1972
Authors: Walter C. Bornemeier
Journal: JAMA (1973)

Almost everyone agrees that the United States could use more physicians. In identifying the specific problems in American health care, the public assigned first place to the physician shortage.<sup>1</sup>Physicians listed it in second place.<sup>1 (p41)</sup>There are a few knowledgeable physicians, however, who warn that constantly urging the universities to produce more doctors might lead to an overproduction. Maybe they are correct. They cite the overproduction of teachers and doctors of philosophy. In spite of the cautious attitude of these physicians, all of the evidence points to a greatly increasing need for more practitioners. A look at the records is convincing. Going back only a few years as a starting point, we find a great increase in almost every physician's ability to cope with disease. The increase in cardiac surgery is phenomenal. Cardiac catheterization is routine today. Electroencephalography is commonplace. Amniocentesis provides much needed information in pregnancy.
234. Long Term Effects of pcDNA3_VEGF165 Intramyocardial Transfer after Experimental Myocardial Infarction in the Rat Scar Tissue Extracellular Matrix
Authors: Fabio D. Mataveli, Sang W. Han, Aline Mendes, Rose Kanashiro, Paulo Tucci, Helena B. Nader, Maria Aparecida S. Pinhal
Journal: Molecular Therapy (2006)

Introduction: After the acute occlusion of a coronary artery, a considerable number of growth factors (GF) are released at the infarct site, triggered by the inflammatory process and the ischemic condition. In this process to compensate regional ischemia, glycosaminoglycans (GAGS) and proteoglycans (PGs) play an important role by stabilizing and protecting GFs from inactivation, promoting GFs binding to its receptors, and facilitating receptor dimerization and signaling. The expression of the Condroitin, Dermatan and Heparan Sulfate (CS, DS and HS), Hyaluronan (HA), the HSPG Syndecan-4 and the enzymes Heparanase and Cathepsin- B in the infarction scar tissue were studied.
Lower socioeconomic status and shorter survival following HIV infection
Authors: R.S Hogg, S.A Strathdee, K.J.P Craib, M.V O'Shaughnessy, J.S.G Montaner, M.T Schechter, R.S Hogg, S.A Strathdee, J.S.G Montaner
Journal: The Lancet (1994)
Hereditary Gustatory Sweating
Authors: J. C. Mailander
Journal: JAMA (1967)

REFLEX SWEATING can be classified broadly into three clearly recognizable categories: emotional, thermal, and gustatory. In this last category are several subgroupings ranging from postencephalitic and syringomyelia gustatory sweating, mentioned in most texts on neurology,<sup>1</sup>to the morefamiliar auriculotemporal syndrome<sup>2-5</sup>and other less-common entities, such as the crocodile tear syndrome,<sup>6,7</sup>submental sweating,<sup>8</sup>and postsympathectomy sweating.<sup>3,9</sup> The auriculotemporal syndrome and most of the less-common entities mentioned above can be explained by the "aberrant regeneration theory" proposed by Ford<sup>6</sup>and reexamined by Glaister et al.<sup>5</sup>In these cases there is always a history of surgery, trauma, or suppurative infection which in some manner damages the nerve axons responsible for salivation and sweating. Subsequently, the regeneration of these axons is aberrant; in other words, the nerve supply to the salivary gland in part grows out to innervate the sweat gland, and vice versa. With this nerve
An unusual case of hepatosplenomegaly
Authors: D Joshi
Journal: Gut (2005)

Background and aims: Although external anal sphincter dysfunction is the major cause of urge faecal incontinence, approximately 50% of such patients have evidence of rectal hypersensitivity and report exaggerated stool frequency and urgency.The contribution of rectosigmoid contractile activity to the pathophysiology of this condition is unclear, and thus the relations between symptoms, rectal sensation, and rectosigmoid motor function were investigated.Methods: Fifty two consecutive patients with urge faecal incontinence, referred to a tertiary surgical centre, and 24 volunteers, underwent comprehensive anorectal physiological investigation, including prolonged rectosigmoid manometry.Patients were classified on the basis of balloon distension thresholds into those with rectal hypersensitivity (n = 27) and those with normal rectal sensation (n = 25).Automated quantitative analysis of overall rectosigmoid contractile activities and, specifically, high amplitude contractions and rectal motor complex activity was performed.Results: External anal sphincter dysfunction was similar in both patient groups.Overall, phasic activity and high amplitude contraction frequency were greater, and rectal motor complex variables significantly altered, in those with rectal hypersensitivity.Symptoms, more prevalent in the rectal hypersensitivity group, were also more often associated with rectosigmoid contractile events.For individuals, reduced compliance and increased rectal motor complex frequency were only observed in patients with rectal hypersensitivity.Conclusions: We have identified a subset of patients with urge faecal incontinence-namely, those with rectal hypersensitivity-who demonstrated increased symptoms, enhanced perception, reduced compliance, and exaggerated rectosigmoid motor activity.Comprehensive assessment of rectosigmoid sensorimotor function, in addition to evaluation of anal function, should be considered in the investigation of patients with urge faecal incontinence.
Role of oxygen radicals generated by NADPH oxidase in apoptosis induced in human leukemia cells.
Authors: W Hiraoka, N Vazquez, W Nieves-Neira, S J Chanock, Y Pommier
Journal: Journal of Clinical Investigation (1998)

We have used a human leukemia cell line that, after homologous recombination knockout of the gp91-phox subunit of the phagocyte respiratory-burst oxidase cytochrome b-558, mimics chronic granulomatous disease (X-CGD) to study the role of oxygen radicals in apoptosis. Camptothecin (CPT), a topoisomerase I inhibitor, induced significantly more apoptosis in PLB-985 cells than in X-CGD cells. Sensitivity to CPT was enhanced after neutrophilic differentiation, but was lost after monocytic differentiation. No difference between the two cell lines was observed after treatment with other apoptosis inducers, including etoposide, ultraviolet radiation, ionizing radiation, hydrogen peroxide, or 7-hydroxystaurosporine. After granulocytic differentiation of both cell lines, CPT still induced apoptosis, suggesting independence from replication in fully differentiated and growth-arrested cells. Pyrrolidine dithiocarbamate (an antioxidant inhibitor of NF-kappaB) and catalase partially inhibited CPT-induced DNA fragmentation in granulocytic-differentiated PLB-985 cells, but had no effect in X-CGD cells. Flow cytometry analysis revealed that reactive oxygen intermediates were generated in CPT-treated PLB-985 cells. These data indicate that oxygen radicals generated by NADPH oxidase may contribute directly or indirectly to CPT-induced apoptosis in human leukemia and in neutrophilic-differentiated cells.
Gallium-67 scanning in pyrexia of unknown origin.
Authors: A J Hilson, M N Maisey
Journal: BMJ (1979)

We believe that hypercalcaemia after tamoxifen may signify tumour response and be a reason for continuing with tamoxifen and treating the hypercalcaemia.This is supported by a report of tamoxifen "flare" in metastatic breast cancer and the observation that acute exacerbation of bone pain in patients with breast cancer after tamoxifen may be associated with tumour response.'5The mechanism of tamoxifeninduced tumour response needs further investigation, since acute hypercalcaemia is not seen in patients responding to chemotherapy or oophorectomy.
Amino Acid Metabolism in Human Cancer Cachexia
Authors: Peter W. T. Pisters, Murray F. Brennan
Journal: Annual Review of Nutrition (1990)

Cancer cachexia is a complex syndrome that occurs with variable incidence in patients with solid tumors and those with hematologic malignancies. It is associated with characteristic physical and laboratory findings, and at a more fundamental level, with significant abnormalities in carbohydrate, lipid, and protein metabolism. These alterations in intermediary metabolism are demonstrable early in the syndrome, even before the onset of weight loss, when the more characteristic features of cancer cachexia are evident. Progressive wasting of peripheral protein stores is a major feature of cancer cachexia and often one of the most graphic realities of malignancy for patients and their families. Unfortunately, significant problems with the animal models of cancer cachexia make conclusions derived from animal studies difficult to extrapolate to humans. Data from human studies indicate that human cancer cachexia is associated with minimal aberrations in circulating free amino acid concentrations; increased whole-body protein turnover, synthesis, and catabolism; reduced rates of skeletal muscle protein synthesis; and increased rates of hepatic protein synthesis. Whether or not these alterations represent pathologic responses or physiologic adaptation by the host to the presence of malignancy remains to be seen. Future investigations must focus on more careful evaluation of interorgan amino acid metabolism, investigation of skeletal muscle protein catabolic rates in cancer cachexia, and definition of the roles of altered hormonal and cytokine regulation of these processes. Such studies will more precisely define the level at which amino acid metabolism is altered significantly and, we hope, permit more specific therapeutic intervention designed to reverse the debilitating effects of cancer cachexia.
Bone Scintigraphy of Hip Prostheses
Authors: D J LI, K A MILES, E P WRAIGHT
Journal: Clinical Nuclear Medicine (1994)

The patterns of abnormality (both site and intensity of uptake) on bone scintigraphy in 76 total hip replacements were studied and graded to determine whether specific patterns of abnormality were associated with greater accuracy in deciding the need for revision surgery. Patients were divided into three groups: 1) 45 hips were considered to have required surgery, 2) in 15 hips surgery was retrospectively considered unnecessary, and 3) 16 prostheses were painless. There was a trend for fewer abnormal sites and a lower intensity of abnormality from group 1 to group 3. By considering patterns of uptake, the specificity of bone scintigraphy could be improved from 65% to 95%, but at the expense of lower sensitivity, falling from 90% to 75%. The most accurate criteria for determining the need for revision surgery (sensitivity 88%, specificity 85%) were increased uptake at the tip or a total scintigraphy score of greater than 3.
Letter: Forecasting subarachnoid haemorrhage.
Authors: B D Olivarius, M Buhl, H H Hansen
Journal: BMJ (1975)

Keratoconjunctivitis Caused by AdenovirusType 19 SIR,-We were interested in the cases of adenovirus type 19 keratoconjunctivitis reported from Belgium and Holland by Dr. J. Desmyter and others (16 November, p. 406).Though a few strains of this virus have been isolated elsewhere in Britain during 1973-4, it was not until July 1974 that it was first detected in Scotland.The patient, a male anaesthetist aged 52 years, first developed photophobia and "gritty" discomfort of the right eye and five to six days later the left eye became affected.Ophthalmological examination confirmed the diagnosis of keratoconjunctivitis with deep scleral inflammation.A conjunctival swab was submitted for virological examination and meanwhile treatment with chloram- phenicol was instituted, with no effect.Though the episode lasted one month, the patient continued his normal duties throughout.An adenovirus, subsequently identified as type 19, was isolated in human amnion cell cultures.This appeared to be an isolated incident.There was no relevant history of recent travel abroad or contact with a similar case.However, this does raise the question whether anaesthetists may be at special risk from their proximity to the face and oronasal secretions of their patients.-Weare, etc.,