tbuckley/cabot-search huggingface dataset:
FLT3-ITD-TKD dual mutants associated with AML confer resistance to FLT3 PTK inhibitors and cytotoxic agents by overexpression of Bcl-x(L)
Authors: Ksenia Bagrintseva, Stefanie Geisenhof, Ruth Kern, Sabine Eichenlaub, Carola Reindl, Joachim W. Ellwart, Wolfgang Hiddemann, Karsten Spiekermann
Journal: Blood (2004)
Clindamycin-resistant Staphylococcus aureus in foot ulcers of patients with diabetes
Authors: M.V. Regeer, A.C. van Bon, L. Spanjaard, T.E. Busch-Westbroek, C.H.M. van Schie, S.E. Geerlings, J.H. DeVries
Journal: Journal of Infection (2010)
Gastrointestinal Bleeding in an Incarcerated Hiatal Hernia
Authors: SAM KRINSKY, RUDOLPH SCHILLI
Journal: Clinical Nuclear Medicine (1985)

An example of upper Gl bleeding in an incarcerated hiatal hernia is presented. The patient was sent, while bleeding, to Nuclear Medicine for a radioisotope bleeding scan that revealed a circular collection of radioactivity posterior to the myocardium. The impression concerning this accumulation was that it might be bleeding in a hiatal hernia, bleeding esophageal varices, or an aneurysm of the descending aorta. An esophagram and upper Gl series demonstrated a definite incarcerated hiatal hernia corresponding to the nuclear scintigraphic finding. In addition, upper endoscopy showed a large hiatal hernia containing several discrete erosions that were not bleeding actively.
Disulfide-Bond Reshuffling in the Evolution of an Ape Placental Ribonuclease
Authors: Jianzhi Zhang
Journal: Molecular Biology and Evolution (2006)

Disulfide bonds play important roles in the folding and stability of proteins and are evolutionarily conserved. A classic example is RNase A (also known as bovine pancreatic ribonuclease), which contains 4 conserved disulfide bonds among 8 cysteines. However, human RNase 8, a paralog of RNase A uniquely expressed in the placenta, has lost one of the conserved cysteines but gained another, when compared with RNase 8 of various monkeys and with RNase A. We here show that both the loss and gain of the cysteines in human RNase 8 occurred in the common ancestor of African great apes (humans, chimps, and gorillas) 7-13 MYA. Computational predictions suggest changes of disulfide bonding by these cysteine substitutions. Site-directed mutagenesis indicates that if the ribonucleolytic activity is essential for RNase 8's function, the gain of the cysteine must have preceded the loss. Human RNase 8 represents one of the first examples in which the presumable evolutionary change of a disulfide bond involves 1 loss and 1 gain of cysteine, instead of 2 losses or 2 gains. Our results provide the foundation for detailed analysis toward understanding the impact of disulfide-bond reshuffling on the structure, function, and evolution of proteins in general and human RNase 8 in particular.
Importance of Blinding in Randomized Trials
Authors: Sheri A. Strite, Michael E. Stuart
Journal: JAMA (2010)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Mycophenolate mofetil (MMF) monotherapy improves renal dysfunction in orthotopic liver transplant (OLT) recipients without increased risk of rejection
Authors: Maria Luisa Raimondo, Lucy Dagher, George Papatheodoridis, Fulya Gunsar, Valerio Turchi, Keith Rolles, Brian Davidson, David Patch, Andrew Burroughs
Journal: Journal of Hepatology (2002)
Circulating Tumor Microemboli Diagnostics for Patients with Non–Small-Cell Lung Cancer
Authors: Anders Carlsson, Viswam S. Nair, Madelyn S. Luttgen, Khun Visith Keu, George Horng, Minal Vasanawala, Anand Kolatkar, Mehran Jamali, Andrei H. Iagaru, Ware Kuschner, Billy W. Loo, Joseph B. Shrager, Kelly Bethel, Carl K. Hoh, Lyudmila Bazhenova, Jorge Nieva, Peter Kuhn, Sanjiv S. Gambhir
Journal: Journal of Thoracic Oncology (2014)
Bathing in hospital.
Authors: M A Chamberlain, J Stowe
Journal: BMJ (1982)

On entering hospital the person becomes the patient, loses most of his independence, and is subjected to numerous investigations. To what extent do patients retain their dignity and independence in the use of bathroom and lavatory facilities while in hospital ? Does the ward environment aid or impede the maintenance or development of independence of those with disabilities (whether longstanding or of recent origin) ? To answer these questions we investigated the facilities at a large teaching hospital in Leeds to discover whether patients can bath and go to the lavatory as independently as possible.
Abstract 348: LKB1 deficiency confers lung adenocarcinoma phenotypic plasticity with squamous transdifferentiation potential.
Authors: Xiangkun Han, Fuming Li, Zhaoyuan Fang, Yijun Gao, Rong Fang, Fei Li, Yihua Sun, Jufeng Xia, Shun Yao, Li Li, Haiquan Chen, Yingyong Hou, Xinyuan Liu, Hongbin Ji
Journal: Cancer Research (2013)

Abstract The existence and related mechanisms of phenotypic plasticity and dynamic lineage conversion of lung cancers, as implicated by clinical observation of mixed adenocarcinoma (ADC) and squamous cell carcinoma (SCC) pathologies in adenosquamous cell carcinoma (Ad-SCC), remain as fundamental yet unsolved questions. Here we find that about 13% human lung Ad-SCC harbor genetic alterations of tumor suppressor LKB1. Through lineage tracing experiments and pathological analyses in mouse models of human lung cancer, we further show that LKB1 deficiency confers lung adenocarcinoma phenotypic plasticity to progressively transdifferentiate into squamous cell carcinoma (SCC), via pathologically mixed Ad-SCC as intermediate. Down-regulation of lysyl oxidase (LOX) following reduced hypoxia level in LKB1-deficient lung adenocarcinoma decreases collagen disposition and triggers extracellular matrix remodeling and eventually up-regulates p63 expression, the SCC lineage survival oncogene. Moreover, pharmacological inhibition of Lox enzymatic activity promotes the transdifferentiation, whereas ectopic Lox expression conversely increases collagen deposition and significantly inhibits this process. ADC and SCC show differential response to pharmacological Lox inhibition. Notably, human lung Ad-SCC with LKB1 genetic alterations show pathology-specific patterns of LOX expression and collagen deposition in adenomatous and squamous portions as seen from animal models. These data uncover an essential role and related molecular mechanism of LKB1 deficiency in determining lung adenocarcinoma phenotypic plasticity. Citation Format: Xiangkun Han, Fuming Li, Zhaoyuan Fang, Yijun Gao, Rong Fang, Fei Li, Yihua Sun, Jufeng Xia, Shun Yao, Li Li, Haiquan Chen, Yingyong Hou, Xinyuan Liu, Hongbin Ji. LKB1 deficiency confers lung adenocarcinoma phenotypic plasticity with squamous transdifferentiation potential. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 348. doi:10.1158/1538-7445.AM2013-348
Abstract 48: A novel method for live cell sorting based on RNA expression levels of cancer biomarkers maintains normal cellular function as confirmed by downstream functional testing
Authors: Don Weldon, Victor Koong, Yuko Williams
Journal: Cancer Research (2014)

Abstract Enrichment of cells within a mixed population has been confined to fixed cells or in the case of live cell sorting to a limited set of cell surface markers which are typically not specific to the target population. Furthermore, cell surface antibodies may activate cell surface receptors altering the state of the cells such that they are no longer representative of the original population. Intracellular staining requires fixation and permeabilization and thus renders the cells unusable in downstream functional assays. Through the use of a unique nanoparticle sequence based detection technology we have shown the ability to detect specific RNA within live cells which does not affect or alter the cells and allows further testing of the target population following the enrichment. Both microRNA and mRNA species have been used to enrich for specific target populations through Fluorescence Activated Cell Sorting (FACS). Sorting cells based on the expression level of any specific RNA allows researchers the ability to utilize targets of biological interest within isolated populations of cells. Since this method is non-destructive to live cells the enriched cells can then be used for downstream functional assays. Here we present data showing the live cell enrichment of a target cell population within a heterogeneous mixture of cells based on their RNA expression levels. RNA probes to miR-221 and miR-222 were generated for the enrichment of invasive cells from the non-invasive. A mixture of breast cancer cells (MD-MB-231 and T47D) were sorted based on their expression levels of the RNA probes through FACS. The high and low expressing sort products were then each subjected to a collagen invasion assay. As expected the fractions which showed high expression of miR-221 and miR-222 showed a high level of invasion through the matrix while the low expressing fractions showed extremely low invasion. Live cell sorting based on RNA levels of cancer biomarkers in a non-destructive manner enables researchers the ability to study the expression of a diverse group of targets beyond the traditional surface markers with the added benefit of utilizing the enriched cells for downstream functional assays. Citation Format: Don Weldon, Victor Koong, Yuko Williams. A novel method for live cell sorting based on RNA expression levels of cancer biomarkers maintains normal cellular function as confirmed by downstream functional testing. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 48. doi:10.1158/1538-7445.AM2014-48
Hypocapnia-induced ventilation/perfusion mismatch: a direct CO2 or pH-mediated effect?
Authors: K B Domino, E R Swenson, M P Hlastala
Journal: American Journal of Respiratory and Critical Care Medicine (1995)

The purpose of this study was to determine whether the increased ventilation/perfusion (VA/Q) mismatch caused by hypocapnic hyperventilation in dogs (J. Appl. Physiol. 1993; 74:1306-1314) is a direct CO2 or a pH-mediated effect. From an initial state of hyperventilated respiratory alkalosis (FIO2 = 0.21, VT = 18 ml/kg, RR = 35), we studied the changes in VA/Q distributions, respiratory gas exchange, and hemodynamics when the acid-base status of the dogs was manipulated by combinations of acid or alkali infusion with or without CO2 inhalation. In this manner, we studied respiratory alkalosis (high pH, low PCO2), normalized acid-base status (normal pH, normal PCO2), metabolic acidosis (low pH, normal PCO2), metabolic alkalosis (high pH, normal PCO2), and a mixed respiratory alkalosis and metabolic acidosis (normal pH, low PCO2). Gas exchange was evaluated using the multiple inert gas elimination technique. PaO2 was reduced and VA/Q heterogeneity was increased in all conditions defined by a high pH, independent of the PCO2 (respiratory alkalosis and metabolic alkalosis). In contrast, PaO2 and VA/Q heterogeneity was unchanged in conditions defined by either a normal or low pH (normalized acid-base status, mixed respiratory alkalosis and metabolic acidosis, and metabolic acidosis). Therefore, we conclude that hypocapnia-induced VA/Q mismatch in hyperventilated dogs is pH-mediated and is not a function of PCO2 per se.
Diagnostic Accuracy of Cytology in Oat-Cell Carcinoma
Authors: WILLIAM J. FRABLE
Journal: Annals of Internal Medicine (1984)

Letters and Corrections1 June 1984Diagnostic Accuracy of Cytology in Oat-Cell CarcinomaWILLIAM J. FRABLE, M.D.WILLIAM J. FRABLE, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-100-6-918_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: I have just finished giving expert testimony in a medical malpractice case involving a pathologist who apparently made an erroneous diagnosis of oat-cell carcinoma of the lung from a single bronchial brushing from a patient who had a peripheral lung mass. On the basis of that single positive report, an oncologist had submitted the patient for 14 months of chemotherapy. At that time, I reviewed the slides on referral and believed that they showed reserve cell hyperplasia.I am writing specifically in regard to the expert testimony given by the plaintiff's witness, an assistant professor in pulmonary...References1. GRINERMAYEWSKIMUSHLINGREENLAND PRAP. Selection and interpretation of diagnostic tests and procedures: principles and applications. Ann Intern Med. 1981;94(4 pt 2):553-600. Google Scholar2. PAYNESTOVINBARKERMCVITTIESTARK CPVSJ. Diagnostic accuracy of cytology and biopsy in primary bronchial carcinoma. Thorax. 1979;34:294-9. CrossrefMedlineGoogle Scholar1. GRINERMAYEWSKIMUSHLINGREENLAND PRAP. Selection and interpretation of diagnostic tests and procedures: principles and applications. Ann Intern Med. 1981;94:553-600. Google Scholar2. PAYNESTOVINBAKERMCVITTIESTARK CPVSJ. Diagnostic accuracy of cytology and biopsy in primary bronchial carcinoma. Thorax. 1979;34:294-9. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 June 1984Volume 100, Issue 6Page: 918-919KeywordsCell biologyChemotherapyLung and intrathoracic tumorsPathologists Issue Published: 1 June 1984 PDF downloadLoading ...
Combination Chemotherapy for Advanced Breast Cancer: Response and Effect on Survival
Authors: GEORGE P. CANELLOS
Journal: Annals of Internal Medicine (1976)

Forty patients with metastatic breast cancer who had received no previous cytotoxic therapy were treated with a combination chemotherapy program CMF (P), which included methotrexate, 60 mg/m2, and 5-fluorouracil, 700 mg/m2, intravenously on the first and eighth days, in addition to cyclophosphamide, 100 mg/m2, and prednisone, 40 mg/m2, by mouth daily from the first to the fourteenth day of a 28-day cycle. Only 2 of 25 patients responded to hormonal therapy or endocrine ablation. Twenty-seven of the 40 patients (68%) had a complete response (8 patients) or partial response (19 patients). Lung, soft tissue, and nodal metastases were the most responsive sites. The median duration of antitumor response was 8 months, with a median survival of 18 months for the responding group. The nonresponders had a median survival of 4 months. The toxicity was primarily hematologic and was especially severe in patients with functional liver impairment due to metastatic disease.
Awareness during Caesarean Section under General Anaesthesia
Authors: J. Wilson, D. J. Turner
Journal: BMJ (1969)

Investigation of a series of 150 obstetric patients, the majority undergoing caesarean section, showed the expected figure of 2% with factual recall. There was, however, a 17·3% occurrence of unpleasant recall—associated in 10 cases (6·6% of the total) with recall of pain. There was a negative correlation between the giving of a narcotic within six hours of the operation and the occurrence of unpleasant recall. Several other aetiological factors—age, parity, preoperative emotional tension, ventilation, nitrous oxide wash-out with oxygen, and nitrous oxide concentration—were investigated and no relation was found between them and unpleasant recall. It is suggested, therefore, that premedication still has an important function in light anaesthesia, using muscle relaxants, to prevent any form of unpleasant operative awareness.
Abstract 4372: Chronic myeloid leukemia (CML) exosomes promote angiogenesis in a Src-dependent fashion in vitro and in vivo
Authors: Marco Mineo, Simona Taverna, Anna Flugy, Giacomo De Leo, Riccardo Alessandro, Elise C. Kohn
Journal: Cancer Research (2012)

Abstract CML is an uncontrolled proliferation of bone marrow myeloid cells driven by the constitutively active fusion product tyrosine kinase BCR/ABL. Angiogenesis, the formation of new blood vessels from pre-existing vasculature, is newly recognized as a factor in CML progression. Exosomes, released by a broad spectrum of cells, are microvesicles that play an important role in cell-to-cell communication both in physiological and pathological conditions. The role of exosomes released by CML cells in angiogenesis is emerging; however, little is known about the mechanisms involved in this process. We first isolated and characterized exosomes released by K562 CML cells and we demonstrated their ability to stimulate human vascular endothelial cells (HUVECs) tube differentiation on Matrigel. K562 exosomes induced an increase of the cumulative tube length in a dose-dependent manner, with a maximum effect at 10ug/ml (p=0.003). Next, we evaluated the effect on exosome behavior of imatinib and dasatinib, two tyrosine kinase inhibitors in use in CML treatment. K562 CML cell treatment with either imatinib or dasatinib reduced exosome release by 58% and 56%, respectively (p&amp;lt;0.01). Equivalent exosome concentrations isolated from imatinib or dasatinib treated K562 cells maintained their ability to stimulate HUVEC tube formation, compared to those isolated from untreated cells. Dasatinib treatment of HUVECs strongly reduced exosome-induced vascular differentiation (p=0.0002). On the contrary, little effect was observed following treatment with imatinib. K562 exosomes stimulated vascularization when added to Matrigel plug in vivo; angiogenesis was markedly inhibited by oral administration of dasatinib (p&amp;lt;0.01), but not imatinib. Consistent with the differential effects of dasatinib and imatinib, exosome-induced HUVEC Src and FAK phosphorylation was only inhibited by dasatinib. Confocal images showed that both FAK and Src phosphorylation were increased at points of membrane-matrix contact. Immunoblot analysis confirmed that K562 exosomes induced a dasatinib-sensitive phosphorylation of Src and FAK and their downstream effectors, Erk and Akt. Again, imatinib was minimally active against exosome stimulation of HUVEC cell signaling. Thus, K562 CML exosomes stimulate angiogenesis in vitro and in vivo in a dasatinib-sensitive fashion. This credentials exosomes and angiogenesis as molecular targets in CML via activation of Src both in leukemia and its microenvironment. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4372. doi:1538-7445.AM2012-4372
TCT-813 A Novel Method for Evaluation of Polymer Absorption from Sirolimus Eluting Stent and its Influence on Biological Effects
Authors: Bartłomiej Orlik, Piotr P. Buszman, Janusz Kasperczyk, Adam Janas, Michal Jelonek, Agata Krauze, Stefan Samborski, Bogdan Gorycki, Wojciech Wojakowski, Buszman E. Pawel, Krzysztof P. Milewski
Journal: Journal of the American College of Cardiology (2013)
Erythrocyte Reinfusion and Maximal Aerobic Power
Authors: Michael N. Sawka
Journal: JAMA (1987)

Induced erythrocythemia is associated with a variable increase in maximal oxygen uptake. To examine the roles of the magnitude of change in hemoglobin concentration and the individual's initial aerobic fitness in this effect, we combined individual data from our own research and three other studies. In each study freeze-preserved erythrocytes from the product of 2 units of blood were reinfused, and maximal oxygen uptake was measured within 24 to 72 hours after reinfusion. The 30 subjects had an initial aerobic power of 36 to 88 mL of oxygen per kilogram per minute. The combined results from these studies indicate that after erythrocyte reinfusion the increase in hemoglobin concentration is fairly homogeneous (mean +/- SD, 1.36 +/- 0.6 g/dL [13.6 +/- 6 g/L]), nearly all individuals demonstrate an increase in maximal oxygen uptake (0.357 +/- 0.216 L/min), the magnitude of increase in hemoglobin concentration is not related to the magnitude of increase in maximal oxygen uptake, and the magnitude of increase in maximal oxygen uptake is related to the individual's initial aerobic fitness. Individuals with an initial aerobic fitness between 50 and 65 mL X kg-1 X min-1 experience approximately twice the increase in maximal oxygen uptake after erythrocyte reinfusion of individuals with greater fitness and also of lesser fitness.
The George E. Brown Memorial Lecture
Authors: EDGAR V. ALLEN
Journal: Circulation (1950)

In the course of this presentation I hope it has become evident that the professional life of George Brown could well serve as a model for the activities of the American Heart Association, which has made astonishing and gratifying progress in a few years. The advances in physiology, anatomy, surgery and medicine, as related to cardiovascular diseases, are justifiable reasons for pride. Yet pride in the past can produce no results in the future. Progress in the future must be supported by a tripod, such as has supported it in the past; one leg of the tripod is zeal, another is utilization of skill and knowledge, and another is money. We must furnish all three. The unsolved problems are unsolved not only because they are difficult but because we have not yet achieved a coordinated program of investigation. We must abandon all feelings of futility and avoid tedium. I can find no logical reason to believe that any important cardiovascular disease cannot be largely or wholly vanquished. If the conquest of these diseases continues at the rate which characterized the fifteen years just past, within the lifetime of physicians now living there will be no cardiovascular disease which cannot be prevented or corrected. If we remain imbued with untiring resolution to attack vigorously and with persistent zeal the greatest destroyers of health and life in America, such a program will be continued. We must hold forever before ourselves the truism that scientific research is progress. Because this is the George Brown Memorial Lecture, I may appropriately quote from another "Brown," although spelled Browne, Sir Thomas, the author of "Religio Medici," who, in 1643, wrote as follows: "The World which took but six days to make, is like to take six thousand to make out: meanwhile old Truths voted down begin to resume their places, and new ones rise upon us.... Men disparage not Antiquity, who prudently exalt new Enquiries, and make not them the Judges of Truth, who were but fellow Enquirers of it.... Despise not the obliquities of younger ways nor despair of better things whereof there is yet no prospect."