tbuckley/cabot-search huggingface dataset:
Abstract 4107: Human guanylate-binding protein-1 (GBP-1) in colorectal carcinoma
Authors: Nathalie Britzen-Laurent, Karoline Lipnik, Elisabeth Naschberger, Elisabeth Kuhn, Christine Hohenadl, Michael Stürzl
Journal: Cancer Research (2010)

Abstract The human guanylate binding protein 1 (hGBP-1) is a large GTPase which mediates the anti-proliferative and anti-angiogenic effects of pro-inflammatory cytokines (IC), such as IFNγ, TNFα and IL1α/β, on endothelial cells in vitro. The expression of GBP-1 has been investigated in human colorectal carcinoma (CRC). We could show that GBP-1 is expressed in stromal endothelial cells of 32% of the CRC. This expression was correlated with a better outcome for patients with GBP-1 expression in the tumour stroma. A transcriptome analysis revealed that in the tumours, IFN-γ-induced genes including the major antiangiogenic chemokines CXCL9, CXCL10 and CXCL11 were coexpressed with GBP-1, indicating the presence of an active Th-1-like angiostatic immune reaction. However, the presence of GBP-1 was only detected in the stroma, suggesting that in tumour cells the expression of GBP-1 may be suppressed. In order to investigate the effects of GBP-1 on tumour cells, we reintroduced the protein in the DLD-1 colorectal carcinoma cell line, which does not produce GBP-1 in absence or in presence of IFN-γ. The proliferation rate was lower in GBP-1 expressing DLD-1 cells as compared to control cells. In order to better characterize the in vivo anti-tumourigenic effects of GBP-1, we developed a tumour mouse model using the DLD-1 cell line stably expressing GBP-1. Stable DLD-1 clones, which expressed GBP-1, and respective control cells (transfected with the empty vector plasmid) were xenotransplanted into immunodeficient mice. After 36 days, tumour volumes in the GBP-1 group were significantly smaller than in the control group (p&amp;lt;0.001). This experiment was repeated with identical outcome in two different mouse strains. Immunohistochemistry stainings and Western blot analyses of the tumours confirmed that GBP-1 was highly expressed in the GBP-1 group and absent in control tumours. These results show that GBP-1 expression can inhibit tumour growth in an animal mouse model, indicating that tumour cells may have developed mechanisms to inhibit GBP-1 expression in an IFN-γ dominated microenvironment. The responsible mechanisms are investigated in ongoing studies. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4107.
Gates donates $750m, but Global Fund still needs more backers
Authors: A. Gulland
Journal: BMJ (2012)

Supporters of the Global Fund to Fight Aids, Tuberculosis and Malaria are making a renewed call for financial support, to supplement Microsoft founder Bill Gates’s donation of $750m (£480m; €570m).

Mr Gates made his pledge alongside that of Naoto Kan, the former Japanese prime minister, who confirmed that his country, despite last year’s earthquake, tsunami, and nuclear accident, would still donate the $800m it had already promised.

In November the fund announced it would have to suspend its 11th funding round after donor countries scaled back their contributions because of the financial crisis ( BMJ 2011;343:d7755, doi:10.1136/bmj.d7755). The fund’s minimum target was $13bn, but only $11.7bn was pledged.

An emergency conference of donors, hosted by the United States and to be held in July, has been called for by Joanne Carter, a …
Abstract 5385: A novel cell-based kinase assay panel paired with high content phenotypic profiling to correlate kinase inhibitor specificity and cellular activity
Authors: Tom S. Wehrman, Erika A. O'Donnell, M. Jared Lumpe, Benjamin E. Osetek, Peter O. Krutzik
Journal: Cancer Research (2014)

Abstract In many cancers, drugs that target a single kinase often lack efficacy due to the presence of alternate oncogenic signaling pathways that may compensate for the inhibition of the targeted kinase. Although completely non-selective kinase inhibitors are highly toxic, selective and simultaneous inhibition of a number of kinases can lead to greater responses. Here we describe the development of a multiplexed cellular kinase assay platform in which compounds are simultaneously profiled against more than 12 kinase targets in a single sample. Using this high-throughput platform, target activity and selectivity in cells can be optimized concurrently, enabling the selection of specific multi-targeted compounds. Using this platform, 300 kinase inhibitors were fully characterized for cellular activity and potency across a large panel of tyrosine kinases. These inhibitors were then tested across a panel of diverse cancer cell lines in a phenotypic screen for their ability to affect viability, cell cycle, and apoptosis under different growth conditions. The resulting comprehensive data set of existing in vitro data, cellular kinase data, and phenotypic screening results provided novel insights into the relationship between inhibitor selectivity, cell cycle effects, and the correlation to anti-cancer activity. Citation Format: Tom S. Wehrman, Erika A. O'Donnell, M. Jared Lumpe, Benjamin E. Osetek, Peter O. Krutzik. A novel cell-based kinase assay panel paired with high content phenotypic profiling to correlate kinase inhibitor specificity and cellular activity. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5385. doi:10.1158/1538-7445.AM2014-5385
Positron Emission Tomography Analysis of [1- <sup>11</sup> C]Acetate Kinetics in Short-term Hibernating Myocardium
Authors: Rainer Schulz, Christian Kappeler, Heinz Coenen, Andreas Bockisch, Gerd Heusch
Journal: Circulation (1998)

Background —Modeling of the time-[1- 11 C]acetate activity curve assumes a constant concentration of labeled tricarboxylic acid cycle intermediates and associated metabolites, such as glutamate and aspartate, which may, however, decrease in short-term hibernating myocardium. Methods and Results —In 12 anesthetized pigs, [1- 11 C]acetate was injected as a bolus into the cannulated left anterior descending coronary artery during normoperfusion, inotropic stimulation, and early (5 to 45 minutes) and prolonged ischemia (60 to 90 minutes). Regional myocardial oxygen consumption (MV̇ o 2 , microliters per minute per gram) was measured, and the absence of necrosis was verified by triphenyl tetrazolium chloride staining. Inotropic stimulation increased MV̇ o 2 from 52.5±7.4 to 195.4±36.2 (mean±SD) and the rate constant (k mono , minutes −1 ) of [1- 11 C]acetate clearance from 0.094±0.018 to 0.322±0.076. During early ischemia, MV̇ o 2 and k mono were decreased to 24.3±8.5 and 0.061±0.011, respectively. K mono closely correlated to MV̇ o 2 during normoperfusion, inotropic stimulation, and early ischemia. In short-term hibernating myocardium, however, at an unchanged MV̇ o 2 , k mono increased toward control values (0.080±0.014). Myocardial glutamate and aspartate concentrations (biopsies) decreased to 47±26% and 77±18%; the peak count rate decreased to 66±22% of its respective control value. After correction for the decreases in glutamate and aspartate or in peak count rate, k mono was again decreased (0.050±0.016 or 0.052±0.014, respectively), and a close relationship to MV̇ o 2 was restored. Conclusions —K mono correlates to MV̇ o 2 in short-term hibernating myocardium when the decreases in aspartate and glutamate or in peak count rate are considered.
Abstract S5-1: Prospective Outcomes for Patients with Micro-Metastases and Macro-Metastases In Sentinel Nodes: NSABP B-32 Sentinel Node Trial
Authors: TB Julian, SJ Anderson, N Golesorkhi, V Fourchotte, EP Mamounas, N. Wolmark
Journal: Cancer Research (2010)

Abstract Background: Sentinel node biopsy (SNB) allows for a more detailed analysis of axillary nodes, increasing the detection of nodal metastatic disease. Prospective outcome results related to patients with micro-and macro-metastases in sentinel nodes from the NSABP B-32 trial are presented here. Methods: After stratification, women with operable invasive breast cancer and clinically negative nodes were randomly assigned to sentinel node resection (SNR) with immediate conventional axillary dissection (AD) [Group 1] or to SNR without AD [Group 2]. Group 2 patients with positive SNs underwent AD. Sentinel nodes underwent analysis by hematoxylin and eosin. Systemic therapy was at the discretion of the physician. Regional nodal irradiation for node positive lumpectomy patients and chest wall/regional node irradiation for node positive mastectomy patients was permitted. Univariate and multivariate analyses were carried out via Cox PH models to identify predictors of overall survival (OS) and disease-free survival (DFS). Results: From May 1999 through February 2004, 5,611 patients were entered into NSABP B-32. A total 1,390 patients with positive SN were identified; 1,389 of these patients had follow-up data. There were 422 patients with macro-metastases (&amp;gt;2mm), 312 with micro-metastases (&amp;gt;0.2, ≥2.0mm), and 626 with unknown status. Median time in study was 94 months. 97% of SN-positive patients received systemic adjuvant therapy. Significant univariate predictors of OS and DFS were age, receptor status, clinical tumor size, histologic grade, number of positive SNs, SN metastasis size, number of positive nodes, lymphovascular invasion, and systemic therapy. Multivariate analysis identified histologic grade (HR: 2.30, P&amp;lt;0.0001), SN macrometastasis (HR: 2.44, p=0.0003), systemic therapy (HR: 0.22, P&amp;lt;0.0001), age, and clinical tumor size (CTS) as significant predictors of OS (Table 1) with similar predictors for DFS. For DFS, a significant quadratic effect due to age (p=0.015) was observed where younger and older women had higher risk of having an event than did middle aged women. Conclusion: The most striking factors associated with poor OS and DFS were poor histologic tumor grade and macro-versus micro-SN metastases. Patients with micro-or macro-SN metastases who received systemic therapy had a 78% reduction in mortality and a 76% improvement of DFS. Supported by: NCI U10-CA-69651, -12027, -37377, -69974. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr S5-1.
A Case of Traumatic Tetanus Treated by Antitoxin and Morphine: Recovery
Authors: G. P. Goldsmith
Journal: BMJ (1897)

467and most other authors do not mention the paralysis.Hyde, of Chicago, would seem to have seen many cases, and gives an account of the severe symptoms often present-agonising neuralgia, formidable inflammation of all parts of the eye, and falling out of teeth and hair, scars from suppuration of the vesicles, paresis and paralysis with resulting muscular atrophy, years of intense suffering, leaving the patient a physical wreck; and, finally, a rare termination in mening- itis and pywmia.That herpes of the face may thus result in most serious illness and permanent deformity is most im- portant for practitioners to know.These cases are probably much more frequent than the i8 Eichhorst refers to.Two were reported in the Lancet, I894, and I think I have seen others in the medical papers.The two following cases occurred in my practice recently, and may be of interest.CASE I.-Mrs.R., aged 68, complained of severe neuralgic pain on the right side of head, face, and neck, on September 26th, x896.There was no cause for it that she could assign.A red patch, the size of a half-crown, was visible near the centre of right sterno-mastoid, and another further
The Pathology of Hepatitis C
Authors: Peter J. Scheuer, Parvin Ashrafzadeh, Sheila Sherlock, David Brown, Geoffrey M. Dusheiko
Journal: Hepatology (1992)

To determine the histologic pattern of hepatitis C, 54 liver biopsy specimens from 45 patients with a clinicopathological diagnosis of hepatitis C were studied. All patients were seropositive for antibody to hepatitis C virus by second-generation testing. Both transfusion-related and sporadic cases were included. More than half the samples showed chronic hepatitis without cirrhosis, whereas 44% showed developing or fully established cirrhosis. A histological pattern of mild chronic hepatitis with portal lymphoid follicles and varying degrees of lobular activity was found in many of the patients. Lymphoid aggregates or follicles were seen in 78% of biopsy specimens, but aggregates, less prominent than in hepatitis C, were also seen in 14 of 27 samples (52%) from patients with hepatitis B. We conclude that a characteristic histological pattern exists in chronic hepatitis C, that this pattern is not always found and that prominent lymphoid follicles, though not unique to hepatitis C, provide a useful diagnostic clue.
Gallium-67 Localization in a Mycotic Aneurysm of the Thoracic Aorta
Authors: JULIO V. RIVERA, GUMERSINDO BLANCO, MANUEL P??REZ, WILLIAM RU??Z
Journal: Clinical Nuclear Medicine (1985)

Localization of Ga-67 in a mycotic aneurysm of the thoracic aorta in a renal transplant recipient is reported. This procedure complements other imaging methods in the diagnosis of this serious condition and may be preferable to ln-111 leukocytes because of its ready availability.
Labile and Systolic Hypertension: A Reappraisal
Authors: Samuel Vaisrub
Journal: JAMA (1981)

As contrasted with diabetes, in which lability is a serious liability, lability in hypertension is generally viewed as a mitigating factor. Labile hypertension has long been regarded either as a relatively benign manifestation of an exaggerated response to stress or, at worst, as a possible prelude to "fixed" hypertension. Most clinicians thus tend to disregard a single casual elevated blood pressure (BP) reading in favor of a normal basal pressure obtained when the patient is relaxed. Similarly, most epidemiologists exclude the labile variant from their studies on hypertension. In their report on a 20-year biennial follow-up study of a Framingham cohort of 5,209 men and women, Kannel et al<sup>1</sup>cast serious doubts on the innocuousness of labile hypertension. Indeed, they challenge the concept of labile hypertension as a separate entity. In their study, nearly all patients with hypertension demonstrated lability; in fact, the more severe the hypertension, the greater
Growth Hormone in Relation to Growth Disturbances.
Authors: Mary L. Parker
Journal: Annals of Internal Medicine (1964)

s1 February 1964Growth Hormone in Relation to Growth Disturbances.Mary L. Parker, M.D.Mary L. Parker, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-60-2-331_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptPituitary dwarfism is suspected in individuals exhibiting immature skeletal proportions, retardation of linear growth, and comparable retardation of bone maturation. For several years, sulfation factor measurements have been employed in this laboratory in the evaluation of proportionate dwarfism. More recently, the metabolic responses (nitrogen retention and hydroxyproline excretion) to short-term administration of graded doses of human growth hormone, and the serum growth hormone level in response to insulin-induced hypoglycemia have shown promise in establishing the diagnosis of pituitary dwarfism.Treatment of pituitary dwarfism with pituitary growth hormone has been strikingly successful in reducing normal growth rates. Bone maturation is stimulated... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: St. Louis, Missouri PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 February 1964Volume 60, Issue 2_Part_1Page: 331-331KeywordsBoneExcretionGrowth hormoneHuman growth hormoneHypoglycemiaInsulinResearch laboratories Issue Published: 1 February 1964 PDF downloadLoading ...
More than half of NHS trusts need to perform better, says report
Authors: Rebecca Coombes
Journal: BMJ (2007)

The Healthcare Commission, the independent NHS watchdog, has delivered its annual “health check,” which shows an increase in the number of trusts in England providing good or excellent services. However, the performance of more than half of NHS trusts is still either fair or weak. 

As this is the second year of the health check, the report provides the first opportunity to compare NHS performance with the results for 2005-6. All 394 trusts are scored in two main categories: quality of services and financial management. The commission was “concerned” that 20 trusts (5% of the total) were ranked weak in both categories.

On quality of services (such as meeting waiting list targets) 16% of trusts were judged excellent and 30% good, an improvement on the previous year. At the lower end …
PRIMARY ADENOCARCINOMA OF THE APPENDIX
Authors: Stanley E. Lawton
Journal: JAMA (1952)

Primary adenocarcinoma of the appendix is a disease rarely seen by individual surgeons and seldom encountered in large clinics. It is perhaps even rarer than is generally supposed, since many cases presented in the literature may not have been carefully screened. Included in some reports are carcinomas arising from the adjacent cecum, while in other instances carcinoid tumors have not been differentiated. The true rarity of the disease was emphasized in 1942 by Young and Wyman, 1 who found in the literature only four cases of what they considered unquestionable adenocarcinomas primary in the appendix; to these they added a fifth. A study by Uihlein and McDonald, 2 covering a 30 year period at the Mayo Clinic, produced 5 adenocarcinomas, 12 mucoceles, and 127 carcinoids of the appendix. In Army personnel Ehrlich and Hunter 3 discovered 441 carcinomas of the gastrointestinal tract. Seventy-two of these had arisen in the appendix;
Charcoal and Drug Adsorption
Authors: BRUCE S. CHANG
Journal: Annals of Internal Medicine (1981)

Letters and Corrections1 March 1981Charcoal and Drug AdsorptionBRUCE S. CHANG, M.D.BRUCE S. CHANG, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-94-3-416_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: The report by Pederson and colleagues (1) of pruritus in chronic hemodialysis patients relieved by activated charcoal therapy is welcome news. The authors correctly note that the symptom is common, and, as suggested by the number of other therapies previously reported, treatment remains imperfect. Activated charcoal appears to be a benign substance that will have widespread application if others find it to be as efficacious as the authors.I wish, however, to call attention to a potential problem the authors did not examine. While I was inquiring as to the availability of activated charcoal preparations in our...References1. PEDERSONMATTERCZERWINSKILLACH JBAF. Relief of idiopathic generalized pruritus in dialysis patients treated with activated oral charcoal. Ann Intern Med. 1980;93:446-8. LinkGoogle Scholar2. SWINYARD E. Surface-acting drugs. In: GOODMAN LS, GILMAN A, eds. The Pharmacological Basis of Therapeutics. New York: MacMillan Publishing Co.; 1975:949. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: BRUCE S. CHANG, M.D.Affiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 March 1981Volume 94, Issue 3Page: 416-416KeywordsDrug therapyPruritus ePublished: 1 December 2008 Issue Published: 1 March 1981 PDF downloadLoading ...
Chlorphenesin for Trigeminal Neuralgia
Authors: Donald J. Dalessio
Journal: JAMA (1973)

To the Editor.—  I wish to call attention to the beneficial effects of chlorphenesin carbamate (Maolate) in the medical treatment of trigeminal neuralgia. This is a centrally acting drug that depresses internuncial spinal neurons and presumably reduces multisynaptic spinal reflexes.  In four patients with trigeminal neuralgia, use of this medication has resulted in relatively prompt reduction in the sensitivity of facial trigger zones. These events were followed in 24 to 48 hours by loss of the typical lancinating pain that characterizes trigeminal neuralgia. The usual dose of chlorphenesin was 400 mg on awakening, 400 mg at midday, and 800 mg at bedtime. After the pain syndrome was suppressed, the dosage was gradually tapered to the lowest dosage consonant with pain relief.  In addition, two patients with trigeminal neuralgia only partially controlled with carbamazepine or diphenylhydantoin, or both, have had marked improvement after addition of chlorphenesin to their regimens. It is
The Role of mel-18, a Mammalian Polycomb Group Gene, during IL-7–Dependent Proliferation of Lymphocyte Precursors
Authors: Takeshi Akasaka, Koh-ichiro Tsuji, Hiroshi Kawahira, Masamoto Kanno, Ken-ichi Harigaya, Lina Hu, Yasuhiro Ebihara, Tatsutoshi Nakahata, Osamu Tetsu, Masaru Taniguchi, Haruhiko Koseki
Journal: Immunity (1997)
The care of children in hospital.
Authors: G Komrower
Journal: BMJ (1979)

The Radcliffe hospitals, Oxford Si?n G Rces, bm;WB Wright, frcped. . . . 673 The younger disabled unit at Fazakerley Hospital R Mulroy, mb. 673 Tuberculosis N Taylor. 673 A case of haemolytic uraemic syndrome A T Coopland, md. 674 Too few necropsies G A C Summers, frcpath. 674 Abnormal cilia B A Afzelius, md. 674 Umbilical hernia and abdominal cramp in swimmers H N C Bleasdale, MB; P T Penny, MB. 674 Man Against Disease D W Costain, mrcpsych. 675 Social factors in marital pathology D A Jennings, bm. 675 Muslims, Ramadan, and diabetes mellitus S G Barber, mrcp, and A D Wright, rcp(c) . 675 Training post, disillusioned registrars and a glut of doctors M G A Palazzo, mrcp. 675 CCHMS proposals J W Stephenson, frcs; B A Ross, frcs. . . . 675 Private referrals J D Williamson, mrcgp. 676 Survival of the fittest? C A Wells, frcs. 676