tbuckley/cabot-search huggingface dataset:
Virus Infection of Endothelial Cells Increases Granulocyte Adherence
Authors: Rob Roy MacGregor, Harvey M. Friedman, Edward J. Macarak, Nicholas A. Kefalides
Journal: Journal of Clinical Investigation (1980)

Adherence of human granulocytes was measured on endothelial monolayers of human and bovine origin, grown in 35-mm Diam petri dishes and in cluster wells. Adherence to human endothelium in petri dishes using 1.0 ml of whole blood averaged 17.9±3.7%, and to bovine endothelium was 20.3±3.7%. Cluster wells required only 1/5 the endothelial cells needed for petri dishes, and 0.25 ml of whole blood yielded average adherence of 26.2±3.4 to human cells and 28.0±3.7 to bovine in the wells. The impact of infection of the endothelium by different viruses on subsequent granulocyte adherence was measured. Polio virus produced an acute lytic infection of human endothelial cells, with associated increased adherence to 185.4% of control 24 h after inoculation. Significantly increased adherence was noted at 6 h, before detectable cytopathic effect. Herpes simplex type I caused a similar rapidly lytic infection of bovine endothelium associated with increased adherence to 213.7% of control 6 h after inoculation. This augmented adherence could be demonstrated when granulocytes were suspended in physiologic saline solution, showing that antibody and complement need not be present. Trypsin treatment of infected monolayers did not prevent the augmentation, and supernate from infected monolayers increased the adherence of polymorphonuclear leukocytes to normal, uninfected monolayers. Chronic, slowly lytic infections, lasting 7 d or more, were induced with adenovirus in human endothelium and with measles virus in bovine cells. Adherence increased as virus was noted in the cell cultures on day 4, several days before cytotoxicity was seen. Thus, chronic viral infection of the endothelium appears possible, and results in increased granulocyte adherence. In naturally occurring disease, such an infection may act synergistically with adherent granulocytes to damage the endothelium, and may represent an in vitro model of vasculitis.
Isolation and characterization of mutations in the human holocarboxylase synthetase cDNA
Authors: Yoichi Suzuki, Yoko Aoki, Yoshinori Ishida, Yasushi Chiba, Akihiro Iwamatsu, Tatsuya Kishino, Norio Niikawa, Yoichi Matsubara, Kuniaki Narisawa
Journal: Nature Genetics (1994)
Vaccinia DNA in Blood After Smallpox Vaccination
Authors: Mary M. Klote, Renata J. M. Engler, Bryan L. Martin, James F. Cummings, Glenn W. Wortmann, George V. Ludwig
Journal: JAMA (2006)

To the Editor: In their Research Letter, Dr Savona and colleagues1 reported on the detection of vaccinia virus DNA in blood and oropharyngeal specimens by polymerase chain reaction (PCR) assay. We believe that to put their results into context, results of other studies must also be considered.
How to Keep Up with the Medical Literature: II. Deciding Which Journals to Read Regularly
Authors: R. BRIAN HAYNES
Journal: Annals of Internal Medicine (1986)

For practitioners, one of the major objectives for reading the medical literature is to maintain clinical competence. Ideally, this task is accomplished through efficiently extracting from the literature properly validated advances in medical knowledge of direct relevance to the reader's own practice. Practically, the extraction process is a difficult one because reports describing such advances are disseminated through a multitude of general and specialty journals. We describe a preemptive strategy for clinicians to determine which journals to read on a regular basis. General and specialty journals of potential relevance to the reader's practice should be selected initially on the basis of circulation or citation impact, and then consecutive issues surveyed to determine the journals' yields of articles that are both directly relevant and of high quality. Subsequent reading should concentrate on the journals that produce the highest yield on this personal survey.
Danazol and Myelodysplasic Syndromes
Authors: ABRAHAM KORNBERG
Journal: Annals of Internal Medicine (1986)

Letters and Corrections1 March 1986Danazol and Myelodysplasic SyndromesABRAHAM KORNBERG, M.D., RIVKA YONA, M.D., SANDA IUKLEA, M.D., SUZANA KAUFMAN, M.D.ABRAHAM KORNBERG, M.D.Search for more papers by this author, RIVKA YONA, M.D.Search for more papers by this author, SANDA IUKLEA, M.D.Search for more papers by this author, SUZANA KAUFMAN, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-104-3-445_3 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: The successful treatment with danazol of three patients with myelodysplastic syndromes and severe thrombocytopenia was described recently by Cines and coworkers (1). After administration of danazol, the platelet count of each patient rose and clinical bleeding stopped. We have had a similar experience with one patient.During 1985 we treated seven patients with myelodysplastic syndromes with danazol, 600 mg/d, for 2 to 4 months. Hemoglobin levels were 5 to 10.1 g/dL, leukocyte counts were 1500 to 6400/mm3, and platelet counts were 14 000 to 132 000/mm3. Previous treatment with oxymetholone, nandrolone phenpropionate, folic acid, pyridoxine phosphate, and...Reference1. CINESCASSILETHKISS DPJ. Danazol therapy in myelodysplasia. Ann Intern Med. 1985;103:58-60. LinkGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: "Assaf Harofeh" Medical Center Zerifin, Beer-Yakov 70300 Israel PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byDanazol for the treatment of thrombocytopenia in patients with myelodysplastic syndromeTherapeutic efficacy of danazol in myelodysplastic syndromesDanazol and Erythema MultiformeLeonard E. Gately III, MD, W. Abe Andes, MDDanazol in the treatment of myelodysplastic syndromes 1 March 1986Volume 104, Issue 3Page: 445-446KeywordsFolic acidHemoglobinHemorrhageMyelodysplastic syndromesPhosphatesPlateletsPyridoxine Issue Published: 1 March 1986 PDF downloadLoading ...
FOXOs and Sirtuins in Vascular Growth, Maintenance, and Aging
Authors: Mark F. Oellerich, Michael Potente
Journal: Circulation Research (2012)

Blood vessels form the first organ in the developing embryo and build extensive networks that supply all cells with nutrients and oxygen throughout life. As blood vessels get older, they often become abnormal in structure and function, thereby contributing to numerous age-associated diseases including ischemic heart and brain disease, neurodegeneration, or cancer. First described as regulators of the aging process in invertebrate model organisms, Forkhead box "O" (FOXO) transcription factors and sirtuin deacetylases are now emerging as key regulators of mammalian vascular development and disease. The integration of individual FOXO and sirtuin family members into various aspects of vessel growth, maintenance, and function provides new perspectives on disease mechanisms of aging, the most important risk factor for medical maladies of the vascular system.
DISPOSITION OF URETERS IN CERTAIN ABNORMAL CONDITIONS OF THE URINARY BLADDER
Authors: WILLIAM E. LOWER
Journal: JAMA (1923)

In the majority of cases, the condition for the relief of which transplantation of the ureters is indicated is a congenital anomaly known as exstrophy of the bladder; this condition is not progressive, and regarding its causation no information which will affect the condition itself may be expected to be developed. It is estimated that exstrophy of the bladder occurs once in every 30,000 to 50,000 births. If this is a correct estimate, there must be about 2,000 cases of exstrophy in the United States. With one or two exceptions, no single surgeon has had more than a very limited series of cases, so that when the occasional case comes to him he has no basis of judgment as to the best mode of procedure. It follows that in many cases only a plastic operation is performed which closes the external defect but does not relieve the chief source of
Estimation of arterial oxygen tension in adult subjects using a transcutaneous electrode.
Authors: D C Hutchison, G Rocca, D Honeybourne
Journal: Thorax (1981)

Transcutaneous oxygen tension (tcPO2) measured with a heated electrode was compared with arterial oxygen tension (PaO2) in three groups of adult patients-group A: 20 patients with chronic respiratory disease; group B: eight hypothermic patents studied immediately after cardiopulmonary bypass surgery; and group C: 14 patients in an intensive care unit who had in all 35 comparisons of tcPO2 and PaO2. For group A, the relationship of the two methods is given by the regression equation (in mmHg): tcPO2 = 0.88 PaO2 + 5.0 (standard error = 5.2: r = 0.93: p less than 0.001) and for group C tcPO2 = 0.69 PaO2 + 14.9 (standard error =12.0, r = 0.90: p less than 0.001). A method of in vivo calibration using a single arterial blood sample improved the reliability of the method in group C. In the group B patients there was no correlation between tcPO2 and PaO2 even when supplementary heating was applied to the skin. Continuous monitoring can reveal large fluctuations in arterial PO2 which would be missed by the use of intermittent arterial samples. The transcutaneous electrode can be employed usefully in intensive care monitoring of adult patients and in physiological studies where repeated arterial samples would otherwise be required.
Case 14-1971
Authors: Richard C. Cabot, Benjamin Castleman, Betty U. McNeely, Murray L. Janower, John B. Blennerhassett
Journal: New England Journal of Medicine (1971)

Presentation of Case First admission. A 64-year-old man entered the hospital because of progressive dyspnea.He had habitually smoked several packages of cigarettes daily and had a chronic cough productive of small quantities of white-to-gray sputum. For many years he had been occupied with the installation of steam generators, and 36 years before admission he had experienced heavy exposure to silica during the digging of a tunnel. Twenty-four years before entry he underwent a laparotomy for the closure of a perforated peptic ulcer, and shortly thereafter a subtotal gastrectomy was performed. Eleven years before admission palpitation developed abruptly, and . . .
FACTORS IN PLASMA CONCERNED IN NATURAL RESISTANCE TO AN AVIAN MALARIA PARASITE (PLASMODIUM LOPHURAE)
Authors: William Trager, R. Barclay McGhee
Journal: The Journal of Experimental Medicine (1950)

The plasma of adult chickens, when injected into young chicks or chick embryos infected with Plasmodium lophurae, lessened the parasitemia. The substances responsible for this effect were inactivated or removed by the heating of adult chicken plasma for (1/2) hour at 65 degrees C., followed by centrifugation to remove the coagulated material; but they were not affected by heating for (1/2) hour at 56 degrees C. The active materials were present in the euglobulin fraction of hen plasma. In similar experiments with ducks, the plasma from each of a series of adult ducks was tested for its effect on the course of infection in young ducklings. The adult ducks were then inoculated with a large dose of parasites. There was a positive correlation between the effectiveness of a plasma in lessening the parasitemia of ducklings treated with it and the resistance on infection exhibited by the duck from which the plasma had been obtained. More than half of the adult female ducks with an active ovary which were tested, but only one of the males, had effective plasmas and also showed relative resistance to the infection.
THE RESPONSIBILITY OF MEDICAL SCHOOLS TO TEACH PHYSICAL THERAPY
Authors: EBEN CAREY
Journal: JAMA (1942)

The administrators of medical schools have a tremendous responsibility during these times of World War II. They now face an accelerated program to shorten the medical course from four to three years and demands for hours allocated for certain additional courses. These problems are presented in the face of an overcrowded medical curriculum which includes the present four years of educating medical students. We are faced both with a reevaluation and a reemphasis on basic essentials of teaching methods and of the content of the medical courses. During this period of stress it is not my desire to propose an additional burden but to point out the great need that our graduates have, who were yesterday our students, for practical instruction in the indications and contraindications of physical therapeutic methods. Notwithstanding the venerable age of physical therapy and its many worthy uses, it gradually fell into disrepute, and at the
Minimal residual disease levels in bone marrow and peripheral blood are comparable in children with T cell acute lymphoblastic leukemia (ALL), but not in precursor-B-ALL
Authors: VHJ van der Velden, DCH Jacobs, AJM Wijkhuijs, WM Comans-Bitter, MJ Willemse, K Hählen, WA Kamps, ER van Wering, JJM van Dongen
Journal: Leukemia (2002)
Osteopontin/Eta-1 upregulated in Crohn's disease regulates the Th1 immune response
Authors: T Sato
Journal: Gut (2005)

<b>Background and aims:</b> The pathogenesis of Crohn’s disease (CD), a chronic inflammatory bowel disease characterised by a Th1 immune response, remains unclear. Osteopontin (OPN) is a phosphoprotein known as an adhesive bone matrix protein. Recent studies have shown that OPN plays an important role in lymphocyte migration, granuloma formation, and interleukin 12 (IL-12) production. The present study investigated expression and the pathophysiological role of OPN in CD. <b>Methods:</b> Plasma OPN concentration was measured by enzyme linked immunosorbent assay. Expression of OPN in human intestinal mucosa was determined using reverse transcription-polymerase chain reaction and western blot, and localisation of OPN was examined by immunohistochemistry. Expression of integrin β<sub>3</sub>, an OPN receptor, on lamina propria mononuclear cells (LPMC) was assessed by flow cytometry. Functional activation of OPN in LPMC was investigated by measuring the production of cytokines. <b>Results:</b> Plasma OPN concentration was significantly higher in patients with CD compared with normal controls or patients with ulcerative colitis (UC). OPN was upregulated in intestinal mucosa from UC and CD patients. OPN producing cells were epithelial or IgG producing plasma cells, or partial macrophages. OPN was detected in areas surrounding granuloma from mucosa in CD. Integrin β<sub>3</sub> expressing macrophages infiltrated inflamed mucosa in UC and CD; in contrast, there was no expression of integrin β<sub>3</sub> on intestinal macrophages in normal mucosa. OPN induced production of IL-12 from LPMC in CD but not in normal controls or UC. <b>Conclusions:</b> Increased OPN expression facilitates cytokine production and is closely involved in the Th1 immune response associated with CD.
Abstract 2563: Upregulation of miR-155 is associated with cisplatin resistance in the head and neck cancer UMSCC-10B/15S cells
Authors: Ashim Gupta, Shoumin Zhu, Mohit Sachdeva, Krishna Rao, Thomas Robbins, Yin Yuan Mo
Journal: Cancer Research (2010)

Abstract Introduction: Cisplatin combination chemotherapy is the cornerstone of treatment for many cancers including head and neck cancer. These complexes bind to the DNA and form adducts such that they ultimately trigger apoptosis. Although initial platinum responsiveness is usually high, the majority of cancer patients will eventually relapse with cisplatin-resistant disease. Thus, chemotherapy resistance is a major clinical obstacle in the successful treatment of head and neck cancer. This study aims to investigate the role of microRNAs (miRNAs) in cisplatin resistance and to better understand the underlying mechanism of cisplatin resistance in the head and neck cancer UMSCC-10B/15S cells. Methods: To determine whether aberrant miRNA expression is associated with cisplatin resistance in human head and neck cancer cells, we profiled miRNA expression in the cisplatin resistant UMSCC-10b/15S and the parental line UMSCC-10B cells by real-time PCR. Luciferase reporter assays and western blot were used to confirm targets for miR-155. We also suppressed miR-155 by anti-sense locked nucleic acid (LNA) oligo to further determine the role of miR-155 in cisplatin resistance. Results: We showed that several miRNAs were altered in UMSCC-10B/15S and among them, miR-155 was the most significantly upregulated miRNA. This was also confirmed by in situ hybridization. Furthermore, ectopic expression of miR-155 was able to increase the resistance of UMSCC-10B cells to cisplatin, which is likely in part through targeting DMTF1, a tumor suppressor that directly binds to the Arf promoter to activate its expression, thereby inducing p53-dependent cell cycle arrest and apoptosis. To determine whether miR-155 directly targets DMTF1, we cloned 1.2 kb 3′-untranslated region (UTR) of DMTF1 into a luciferase reporter and demonstrated that miR-155 was able to suppress luciferase activity by over 70%. We also showed that the endogenous DMFT1 protein was reduced by over 50% in the miR-155 highly expressed resistant cells compared to the parental sensitive cells. Experiments are under way to determine whether ectopic expression of DMTF1 or its suppression by RNAi impacts cisplatin resistance in these cells. Conclusion: These findings suggest that deregulation of miRNAs is in resistant head and neck cancer cells and miR-155 may play a causal role in cisplatin resistance through targeting the DMTF1 gene. As a result, suppression of miR-155 may provide a strategy to overcome the resistance in head and neck cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2563.
Metabolism of 35S-labelled Antithyroid Drugs in Man
Authors: W. D. Alexander, V. Evans, A. MacAulay, T. F. Gallagher, J. Londono
Journal: BMJ (1969)

Differences in the metabolic fate of antithyroid drugs influence the optimal frequency of administration and their therapeutic efficacy. (35)S propylthiouracil differed from the (35)S imidazoles (carbimazole and methimazole) in the more rapid absorption and excretion and the shorter biological half-life in the plasma of the former. Renal function may have a more important influence on the biological half-life of the drugs than thyroid status. Further work is required to determine the optimal frequency of administration for each compound.
DIABETIC PATIENTS HAVE SIMILAR VEIN GRAFT FAILURE AT 1 YEAR, BUT HIGHER 5-YEAR MORTALITY THAN NON-DIABETIC PATIENTS UNDERGOING CORONARY ARTERY BYPASS SURGERY: INSIGHTS FROM THE PREVENT-IV TRIAL
Authors: Masaya Koshizaka, Gail Hafley, Renato Lopes, Adrian Hernandez, Jennifer Green, Nicholas Kouchoukos, Robert Califf, T. Ferguson, John Alexander
Journal: Journal of the American College of Cardiology (2012)
Abstract 3555: LncRNA-21a is a potential tumor suppressor in breast cancer
Authors: Ramesh Singh, Yin-yuan Mo
Journal: Cancer Research (2014)

Abstract Long non-coding RNA (lncRNA)-21A is a polymerase III driven non-coding RNA consisting of 324 nucleotides in length. LncRNA-21A was originally identified through in silico analysis and its expression was then confirmed in human cell lines. Further study suggested that lncRNA-21A can control the proliferation of human tumor cell lines, possibly through regulation of CENP-F expression. However, little is known whether lncRNA-21A plays a role in breast cancer. In this study, we reported that lncRNA-21A is downregulated in breast cancer, as detected by RT-PCR analysis of breast cancer tissue scan, suggesting its possible role as a tumor suppressor. In consistent with this finding, lncRNA-21A promotes apoptosis and attenuates cell invasion ability in breast cancer cells. Western blot revealed that overexpression of lncRNA-21A can reduce the phosphorylation of Akt in MCF-7 cells. To determine its underlying mechanism, we performed RNA precipitation assays with the synthetic biotin labeled lncRNA-21A RNA probe followed by mass spectrometry analysis, and we identified hnRNP U and EWS as binding partners for lncRNA-21A. Suppression of EWS by RNAi leads to a reduced level of phosphorylated Akt, suggesting its role in lncRNA-21A-medited Akt activity. Of great interest, we found that like microRNAs, lncRNA-21A can also be secreted through exosomes into conditioned medium. Furthermore, the secreted lncRNA-21A from normal breast epithelial cells can be functionally taken up by recipient metastatic breast cancer cells, suppressing the cell invasion. Together, these results suggest that as a potential tumor suppressor, lncRNA-21A may be used by the host through exosome-mediated gene transfer as a defense mechanism against invasive tumor cells. Citation Format: Ramesh Singh, Yin-yuan Mo. LncRNA-21a is a potential tumor suppressor in breast cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3555. doi:10.1158/1538-7445.AM2014-3555
Functional effects of protein kinase C-mediated myofilament phosphorylation in human myocardium
Authors: J VANDERVELDEN, N NAROLSKA, R LAMBERTS, N BOONTJE, A BORBELY, R ZAREMBA, J BRONZWAER, Z PAPP, K JAQUET, W PAULUS
Journal: Cardiovascular Research (2005)

Objective: In human heart failure β-adrenergic-mediated protein kinase A (PKA) activity is down-regulated, while protein kinase C (PKC) activity is up-regulated. PKC-mediated myofilament protein phosphorylation might be detrimental for contractile function in cardiomyopathy. This study was designed to reveal the effects of PKC on myofilament function in human myocardium under basal conditions and upon modulation of protein phosphorylation by PKA and phosphatases.
Flice-Inhibitory Protein Expression during Macrophage Differentiation Confers Resistance to FAS-Mediated Apoptosis
Authors: Harris Perlman, Lisa J. Pagliari, Constantinos Georganas, Toshiaki Mano, Kenneth Walsh, Richard M. Pope
Journal: The Journal of Experimental Medicine (1999)

Macrophages differentiated from circulating peripheral blood monocytes are essential for host immune responses and have been implicated in the pathogenesis of rheumatoid arthritis and atherosclerosis. In contrast to monocytes, macrophages are resistant to Fas-induced cell death by an unknown mechanism. FLICE (Fas-associated death domain–like interleukin 1β–converting enzyme)–inhibitory protein (Flip), a naturally occurring caspase-inhibitory protein that lacks the critical cysteine domain necessary for catalytic activity, is a negative regulator of Fas-induced apoptosis. Here, we show that monocyte differentiation into macrophages was associated with upregulation of Flip and a decrease in Fas-mediated apoptosis. Overexpression of Flip protected monocytes from Fas-mediated apoptosis, whereas acute Flip inhibition in macrophages induced apoptosis. Addition of an antagonistic Fas ligand antibody to Flip antisense–treated macrophages rescued cultures from apoptosis, demonstrating that endogenous Flip blocked Fas-induced cell death. Thus, the expression of Flip in macrophages conferred resistance to Fas-mediated apoptosis, which may contribute to the development of inflammatory disease.
NUT Rearrangement is Uncommon in Human Thymic Epithelial Tumors
Authors: Iacopo Petrini, Christopher A. French, Arun Rajan, Michael J. Cameron, Elaine S. Jaffe, Paolo A. Zucali, Jianwu Xie, Yisong Wang, Giuseppe Giaccone
Journal: Journal of Thoracic Oncology (2012)
Coronary endothelial function in hyperhomocysteinemia: improvement after treatment with folic acid and cobalamin in patients with coronary artery disease
Authors: Frank F Willems, Wim R.M Aengevaeren, Godfried H.J Boers, Henk J Blom, Freek W.A Verheugt
Journal: Journal of the American College of Cardiology (2002)