tbuckley/cabot-search huggingface dataset:
Synergistic antitumor effects of lenalidomide and rituximab on mantle cell lymphoma in vitro and in vivo
Authors: Liang Zhang, Zhengzi Qian, Zhen Cai, Luhong Sun, Huaqing Wang, J. Blake Bartlett, Qing Yi, Michael Wang
Journal: American Journal of Hematology (2009)

Rituximab (RTX), a chimeric anti-CD20 antibody, is associated with direct induction of apoptosis and antibody-dependent cell-mediated cytotoxicity (ADCC) with clinical efficacy in mantle cell lymphoma (MCL). Lenalidomide (LEN), a novel immunomodulatory agent, sensitizes tumor cells and enhances ADCC. Our study attempted to elucidate the mechanism of LEN-enhanced RTX-mediated cytotoxicity of MCL cells. We found that LEN and RTX induced growth inhibition of both cultured and fresh primary MCL cells. LEN enhanced RTX-induced apoptosis via upregulating phosphorylation of c-Jun N-terminal protein kinases (JNK), Bcl-2, Bad; increasing release of cytochrome-c; enhancing activation of caspase-3, -8, -9 and cleavage of PARP. Meanwhile, LEN activated NK cells and increased CD16 expression on CD56(low)CD16(+) NK cells. Whole PBMCs but not NK cell-depleted PBMCs treated with LEN augmented 30% of RTX-dependent cytotoxicity. Daily treatment with LEN increased NK cells by 10-folds in SCID mice, and combination of LEN and RTX decreased tumor burden and prolonged survival of MCL-bearing SCID mice. Taken together, our study demonstrates that LEN plus RTX provides a synergistically therapeutic effect on MCL cells by enhancing apoptosis and RTX-dependent NK cell-mediated cytotoxicity and may be an optimal combination in the clinical trial of relapsed or refractory MCL.
5-Azacitidine in aggressive myelodysplastic syndromes regulates chromatin structure at PU.1 gene and cell differentiation capacity
Authors: N Curik, P Burda, K Vargova, V Pospisil, M Belickova, P Vlckova, F Savvulidi, E Necas, H Hajkova, C Haskovec, J Cermak, M Krivjanska, M Trneny, P Laslo, A Jonasova, T Stopka
Journal: Leukemia (2012)
HUMAN AND BOVINE TUBERCULOSIS: THE QUESTION OF MUTATION OF THE TUBERCLE BACILLUS
Authors: N. Raw
Journal: BMJ (1932)

The above summary of etiology anid diagnosis is given, since there will now be described a patient to whom none of the mentioned causes of origin appear to apply, and in whom diagnosis was made only during operation.A man, aged 66, a road sweeper by occupation, presented himself in January, 1929, with the symptoms and signs of benign enlargement of the prostate.This was enucleated in the usual manner by the suprapubic route in a one-stage operation, use being made of a Pilcher bag, which was removed forty-eight hours later.This bag was maintained in position by a cord passing over a pulley to a weight of 1 lb.Five days later severe bleeding occurred, which was controllecl by reintroducing the bag for several hours.The patient made a good recovery, and returned to his work as a road sweeper.
Herb Drinks: Camomile Tea
Authors: Norman R. Farnsworth
Journal: JAMA (1972)

To the Editor.—  The question asked inThe Journal(219:626, 1972), Do herb drinks have less effect on the nervous system than coffee or tea? received, for the most part, a cursory answer. We would like to point out, especially as regards the biological activity of camomile tea ( Matricaria chamomilla ), that from readily accessible literature, M chamomilla contains chamazulene, which has been shown to have antiallergenic as well as anti-inflammatory properties. 1 Further, an en-yn-dicycloether isolated from M chamomilla , has also been shown to elicit anti-inflammatory, anti-anaphylactic, spasmolytic, and bacteriostatic activity. 2 Apigenin-7-D-glucoside has also been shown to contribute to the spasmolytic activity of chamomile preparations. 3   The pure substances mentioned above probably would not exert a significant biological effect at concentrations obtained in a normal human dose of a camomile tea. However, such teas are usually used by the laity over a long period of time, during which a
Targeting of human and mouse T-lymphocytes by monoclonal antibody-HPMA copolymer–doxorubicin conjugates directed against different T-cell surface antigens
Authors: M Jelı́nková, J Strohalm, D Plocová, V Šubr, M Št'astný, K Ulbrich, B Řı́hová
Journal: Journal of Controlled Release (1998)
Reply to E.S. Wang et al
Authors: Mark J. McKeage, Primo N. Lara
Journal: Journal of Clinical Oncology (2012)

We read with interest the correspondence from Wang et al. 1 They and other authors suggest that the optimization of doses, scheduling, and sequencing may have been overlooked during the early develop-ment of ASA404, particularly when the agent was combined with cytotoxic chemotherapy.The ASA404 dose selected for additional development was established after a series of carefully conducted early phase trials. [2]][4] It must be pointed out that the dose (1,800 mg/m 2 ) of ASA404 used
TETANUS WITHOUT EVIDENT PORTAL OF ENTRY
Authors: George W. Goler
Journal: JAMA (1922)

E. W., a man, aged 27, with a negative history, had a root filling put in a tooth, Jan. 3, 1922. January 15, the neck became stiff, and he was unable to open his mouth. On the 16th, he began to have tonic and clonic spasms, involving all the muscles of the body, particularly the extremities and abdomen. Thirst increased, sweating became profuse, and there was great irritability. On the 19th, 11,000 units of tetanus antitoxin was given subcutaneously by a local physician. January 22, he was removed to the Park Avenue Clinical Hospital, Rochester, where a diagnosis of tetanus was made. The spinal canal was tapped, and he was given 4,000 units of tetanus antitoxin intraspinally, 11,000 intravenously and 20,000 subcutaneously. The filling was immediately taken from the dead tooth, which was extracted on the following day. He was given 200 c.c. of a 25 per cent. solution of
Infrared exposure induces an angiogenic switch in human skin that is partially mediated by heat
Authors: M-S. Kim, Y.K. Kim, K.H. Cho, J.H. Chung
Journal: British Journal of Dermatology (2006)

Journal Article Infrared exposure induces an angiogenic switch in human skin that is partially mediated by heat Get access M‐S. Kim, M‐S. Kim Department of Dermatology, Seoul National University College of Medicine, Laboratory of Cutaneous Aging Research, Clinical Research Institute, Seoul National University Hospital, 28 Yongon‐dong, Chongno‐gu, Seoul 110‐744, Korea and Institute of Dermatological Science, Seoul National University, Seoul, Korea Search for other works by this author on: Oxford Academic Google Scholar Y.K. Kim, Y.K. Kim Department of Dermatology, Seoul National University College of Medicine, Laboratory of Cutaneous Aging Research, Clinical Research Institute, Seoul National University Hospital, 28 Yongon‐dong, Chongno‐gu, Seoul 110‐744, Korea and Institute of Dermatological Science, Seoul National University, Seoul, Korea Search for other works by this author on: Oxford Academic Google Scholar K.H. Cho, K.H. Cho Department of Dermatology, Seoul National University College of Medicine, Laboratory of Cutaneous Aging Research, Clinical Research Institute, Seoul National University Hospital, 28 Yongon‐dong, Chongno‐gu, Seoul 110‐744, Korea and Institute of Dermatological Science, Seoul National University, Seoul, Korea Kwang Hyun Cho. E‐mail: khcho@snu.ac.kr and Jin Ho Chung E‐mail: jhchung@snu.ac.kr Search for other works by this author on: Oxford Academic Google Scholar J.H. Chung J.H. Chung Department of Dermatology, Seoul National University College of Medicine, Laboratory of Cutaneous Aging Research, Clinical Research Institute, Seoul National University Hospital, 28 Yongon‐dong, Chongno‐gu, Seoul 110‐744, Korea and Institute of Dermatological Science, Seoul National University, Seoul, Korea Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 155, Issue 6, 1 December 2006, Pages 1131–1138, https://doi.org/10.1111/j.1365-2133.2006.07510.x Published: 01 December 2006 Article history Accepted: 18 May 2006 Published: 01 December 2006
Stroke work or systolic d<i>P</i>/d<i>t</i><sub>max</sub> to evaluate acute response to cardiac resynchronization therapy: are they interchangeable?
Authors: Gerjan de Roest, Paul Knaapen, Marco Götte, Thijs Hendriks, Cor Allaart, Carel de Cock, Albert van Rossum
Journal: European Journal of Heart Failure (2009)
662. The Tracking of Exogenous Cells in the Injured Central Nervous System: Viral Vector Labeling Versus an Intrinsic Genetic Marker
Authors: Bas Blits, Brandon M. Kitay, Andre R. Sanchez, Christian M. Andrade, Damien D. Pearse
Journal: Molecular Therapy (2005)

Top of pageAbstract
Transplantation of exogenous cells into the injured central nervous system and subsequent examination of how these cells survive, integrate and perform physiological functions within the host environment requires that the cells be clearly identifiable from host cells. As many of the cells used for transplantation studies are derived from cultures of cells indistinguishable from the host using immunochemical approaches, numerous dyes or genetic markers have been devised and used for this purpose. These markers used to label and track these transplanted cells need to be specific, reliable, resistant to leakage or cellular transfer, and remain chemically stable once inside the host to allow long-term tracking. In the current study we compared two labeling methods for long-term tracking of cells following transplantation into the injured rat spinal cord; lentiviral vector transduction with green fluorescent protein (GFP) and DNA in situ hybridization for the Y chromosome after male cell transplantation in female rats. Examination of labeled cells at 12 wk post-injury demonstrated that both labels did not exhibit significant fading or loss of the signal due to instability over time. The visualization procedure for the DNA in situ reaction product was significantly more labor intensive than simple identification of GFP by fluorescent microscopy. Y chromosome positive cells were, however, easier to definitely count for assessment of cell survival than GFP labeled cells, due to the presence of the label as a single point within the nucleus rather than diffuse labeling throughout the entirety of the cell. The limited presence of the signal in Y chromosome positive cells though, made examination of their ability to integrate within the injured spinal cord, migrate and associate with axons impossible without additional immunochemical methods for labeling the entire cell. The combination of immunochemistry with DNA in situ however proved to be unsuccessful with the many antibody combinations tested. In conclusion GFP delivery by lentiviral vectors was far superior to DNA in situ detection of the Y chromosome for labeling of transplanted cells due to its rapid visualization and its applicability to a wide range of assessment techniques.
CHRONIC OBSTRUCTIVE MIDDLE-EAR DEAFNESS
Authors: GEORGE E. SHAMBAUGH
Journal: JAMA (1913)

Dr. llaskin's work, which seems to me irrelevant to the sub- ject in baud.Concerning the point that Dr. Loeb raises regarding the diagnosis, it seems to me that there is no reason to assume that the point of irritation is some place distant from that which shows the irritation.This point was once raised by Dr. Scholtz of St. Louis, and since that time I have made the experiment many times, namely, cocainized some other point in the nose which showed no irritation.The result was uniformly a failure.Cocainization of the ganglion proper was uniformly successful in stopping the pain.Concerning what Dr. Dwight asked regarding glaucoma and its association with the sphenopalatine ganglion neural- gia: 1 have been on the lookout for it since 1008 and have seen it in each of the twenty-six cases that I have had the chance to observe.This observation was made primarily by Dr. A. E. ICwing of St. Louis in 1008, but since that time both Dr. Ewing and I have been exceedingly cautious in our statements bearing on these points.In all of these case» the pain of the glaucoma, however, has been uniformly con- trollable from the ganglion.
Language Barriers in Medicine
Authors: Alan Kwasman
Journal: JAMA (1995)

<h3>To the Editor.</h3> —I read with great interest the article by Dr Woloshin and colleagues.<sup>1</sup> However, I believe it should be the patient's responsibility to have an interpreter accompany him or her to each physician visit. Instruction could be written in English, and a copy could be kept in the patient's chart. In my practice in Riverside, Calif, I have patients who speak Spanish, Vietnamese, and Chinese. I cannot afford to hire interpreters for these languages. One alternative physicians have is to deny non—English-speaking patients care. I do not believe that is a reasonable alternative, but I do not feel that the physician should supply the interpreter.
Reducing Cholesterol Levels in Elderly Persons-Reply
Authors: Richard Benfante
Journal: JAMA (1990)

In Reply.—  Dr Brotons and Dr Goldstein have made some excellent points that need to be considered carefully by both public health practitioners and clinicians involved in the prevention of coronary heart disease. It is definitely true that the attributable risk, ie, the absolute difference in rates of disease at a higher level of a risk factor compared with a lower value, provides more information in terms of the actual impact of that risk factor on disease incidence than does the relative risk. In fact, in our study, the attributable risk for the difference between the upper quartile and lower quartiles of serum cholesterol level was approximately 8 per 1000 person-years in the elderly compared with 4 per 1000 person-years in the middleaged men, while the relative risks were about the same.  In reply to Dr Goldstein, we did not mean to suggest how elderly individuals with elevated serum cholesterol
Increased Survival with Prophylactic Quinidine After Experimental Myocardial Infarction
Authors: VICTOR W. HURST, JAMES J. MORRIS, HOWARD J. ZEFT, DONALD B. HACKEL, HENRY D. MCINTOSH
Journal: Circulation (1967)

Experimental myocardial infarction was produced in 60 farm pigs by gradual coronary occlusion with an Ameroid constrictor placed around the left anterior descending coronary artery. Twenty animals were treated with 10 mg/kg/6 hr of quinidine, 10 with 5 mg/kg/6 hr, and 10 with 2.5 mg/kg/6 hr for a total period of 72 hours. Twenty animals served as controls. Serum quinidine levels were measured at 6-hour intervals. Significantly increased survival, when compared to controls, were observed in those animals treated with 5 and 10 mg/kg/6 hr of quinidine, but not in those receiving 2.5 mg/kg/6 hr. Under the described experimental conditions, designed to cause a reproducible, consistent infarction, quinidine treatment in nontoxic and well-tolerated doses significantly increased survival. Furthermore, there is a dose level below which this effect is lost. Whether the results of this study are applicable to man in preventing sudden death is unknown.
Pancreatic β-Cell Dysfunction and Risk of New-Onset Diabetes After Kidney Transplantation
Authors: Dorien M. Zelle, Eva Corpeleijn, Jolijn Deinum, Ronald P. Stolk, Rijk O.B. Gans, Gerjan Navis, Stephan J.L. Bakker
Journal: Diabetes Care (2013)

Chronic exposure to calcineurin inhibitors and corticosteroids poses renal transplant recipients (RTR) at high risk for development of new-onset diabetes after transplantation (NODAT). Pancreatic β-cell dysfunction may be crucial to the pathophysiology of NODAT and specific markers for β-cell dysfunction may have additive value for predicting NODAT in this population. Therefore, we prospectively investigated whether proinsulin, as a marker of pancreatic β-cell dysfunction, is associated with future development of NODAT and improves prediction of it.All RTR between 2001 and 2003 with a functioning graft for ≥1 year were considered eligible for inclusion, except for subjects with diabetes at baseline who were excluded. We recorded incidence of NODAT until April 2012.A total of 487 RTR (age 50 ± 12 years, 55% men) participated at a median time of 6.0 (interquartile range [IQR], 2.6-11.5) years after transplantation. Median fasting proinsulin levels were 16.6 (IQR, 11.0-24.2) pmol/L. During median follow-up for 10.1 (IQR, 9.1-10.4) years, 42 (35%) RTR had development of NODAT in the highest quartile of the distribution of proinsulin versus 34 (9%) in the lowest three quartiles (P < 0.001). In Cox regression analyses, proinsulin (hazard ratio, 2.29; 95% CI, 1.85-2.83; P < 0.001) was strongly associated with NODAT development. This was independent of age, sex, calcineurine inhibitors, prednisolone use, components of the metabolic syndrome, or homeostasis model assessment.In conclusion, fasting proinsulin is strongly associated with NODAT development in RTR. Our results highlight the role of β-cell dysfunction in the pathophysiology of NODAT and indicate the potential value of proinsulin for identification of RTR at increased risk for NODAT.
744-3 Inhibition of Nitric Oxide Synthesis does not Increase Cardiac Contractile Response but Reduces Coronary Blood Flow Response to β-Adrenergic Stimulation in Normal Dogs
Authors: Haruo Kaneko, Takao Endo, Shinsuke Fujita, Kaname Kiuchi, Ikuyo Suzuki, Kuniya Asai, Jun Nejima, Yoshihiro Suzuki, Hirokazu Hayakawa
Journal: Journal of the American College of Cardiology (1995)
Pericardial Effusion Secondary to Severe Hypothyroidism Demonstrated by TI-201 Myocardial Scintigraphy
Authors: CHAN H. PARK, SUNG M. KIM, CHARLES M. INTENZO, JACK MCEWAN
Journal: Clinical Nuclear Medicine (1993)

Pericardial effusion is an uncommon finding in hypothyroidism. A 36-year-old man was noted to have a large pericardial effusion secondary to severe hypothyroidism. He was successfully treated synthroid and gradual disappearance of the pericardial effusion was noted on follow-up examinations.
Oxyphenbutazone-Induced Parotitis
Authors: LUDWIG GROSS
Journal: Annals of Internal Medicine (1969)

Recurrent, febrile parotitis in a 57-year-old woman was associated with repeated administration of oxyphenbutazone (Tandearil®). Only virus serology study may definitely distinguish this malady from epidemic parotitis. Possible mechanism and therapy of this condition are discussed.
Abstract A13: Discovery of selective inhibitors of the kinase and metastatic activities of the activated Cdc42-associated kinase (ACK1)
Authors: Oyenike Olabisi, Vladyslav Kholodovych, Alam Nur-E- Kamal, Safirah Stewart-Singleton, Christina Andayah, Richard Wood, William Welsh
Journal: Clinical Cancer Research (2010)

Abstract Activated Cdc42-associated kinase (ACK1) is a non-receptor tyrosine kinase and an effector protein of Cdc42. Previous reports have demonstrated that ACK1 is a critical factor in the cellular transformation of a number of cancer cell lines. ACK1 also contributes to cellular motility, and overexpression in certain cell lines results in enhanced motility. The significance of tyrosine kinase proteins such as ACK1 in cancer-related signaling pathways, and the implications of metastasis in the prognosis of cancer patients, point to the importance of the discovery of a potent yet selective inhibitor for this protein. As a result, we are engaged in a study focused on applying computational approaches to identify novel inhibitor molecules of ACK that will also hamper its ability to promote motility of cancer cells. We employed customized in-house computational tools to retrieve approximately 50 small-molecule hits from our chemical databases that may bind with high affinity to the ACK1 ATP binding pocket. These hits were further screened for drug-like properties (Lipinski's Rule of 5) and rescored using GOLD docking to the ACK1 crystal structure (PDB3EQR). After rescoring, the top 25 hits were then tested for in vitro cytotoxicity in a variety of cancer cell lines. Compounds were specifically examined for their impact on the kinase activity of purified ACK1 and for their ability to modulate its metastatic properties in wound healing and transwell migration assays. Our screening has revealed that several compounds have the ability to inhibit the in vitro kinase activity of ACK1 and also inhibit the in vitro growth of cancer cells. Ongoing immunofluorescence, cellular transformation, and cellular motility studies suggest that the selected compounds may modulate the effect of ACK1 on the motility of cancer cells. We are actively proceeding with specific structural modifications of our initial inhibitor compounds in order to find molecules which possess increased cytotoxicity to cancer cells and improved specificity for the inhibition of ACK. Citation Information: Clin Cancer Res 2010;16(14 Suppl):A13.
No-Reflow Phenomenon Persists Long-Term After Ischemia/Reperfusion in the Rat and Predicts Infarct Expansion
Authors: Thorsten Reffelmann, Sharon L. Hale, Joan S. Dow, Robert A. Kloner
Journal: Circulation (2003)

No-reflow after reperfusion therapy for myocardial infarction is a strong predictor of clinical outcome. But its fate on a long-term basis and potential significance for infarct healing are not yet known.Twenty-nine female Fisher rats were subjected to 60 minutes of coronary occlusion followed by reperfusion. At 4 weeks, 15 survivors were euthanized after measurement of regional myocardial blood flow (radioactive microspheres) and in vivo staining of perfused tissue (0.5 mL 50% Uniperse blue IV). Infarct size (34.3+/-3.4%), scar thickness (1.19+/-0.10 mm), and infarct expansion index (0.51+/-0.04) were assessed from histological sections (2 additional exclusions because of failed occlusion). Regional myocardial blood flow in the reperfused infarct was reduced significantly compared with noninfarcted tissue (1.98+/-0.47 versus 4.55+/-0.86 mL x min(-1) x g(-1), P<0.003, apical slice, and 1.77+/-0.44 versus 5.34+/-0.38 mL x min(-1) x g(-1), P<0.0001, second slice), accompanied by a striking reduction of perfused capillaries within the infarct (n=23+/-4 versus 163+/-8 in the noninfarcted tissue, P<0.0001, microscopically assessed as capillaries containing blue particles per high-power field). Macroscopically, no-reflow areas were visible in 9 of 13 hearts. The number of perfused capillaries within the infarct correlated significantly with infarct expansion index (r=-0.76, P<0.003), infarct thickness (r=0.60, P<0.03), and the ratio of infarct to septum thickness (r=0.74, P<0.004).The no-reflow phenomenon persists for 1 month after reperfusion and predicts worse scar thinning and infarct expansion. Thus, one might shift the "open-artery" hypothesis downstream to an "open-microvessel" hypothesis, relating infarct healing, infarct expansion, and outcome to the completeness of microvascular reperfusion above and beyond epicardial artery patency.
Atypical or Suspicious Cervical Smears
Authors: Istvan Nyirjesy
Journal: JAMA (1972)

In a study of 26,745 screening examinations, 434 patients with atypical and 95 patients with suspicious cervical cytosmears were identified. With specific antivaginitis treatment 84% of the findings from smears reverted from atypical to negative; patients with second smears showing atypical results were subjected to biopsies that yielded cancer in 18 and dysplasia in 26 of 57 patients. Biopsy specimens verified cancer in 40 and dysplasia in 26 of the 79 patients with initial suspicious smears. Repeat smears taken immediately prior to biopsies were false-negative in 19 of these patients.