tbuckley/cabot-search huggingface dataset:
Abstract 2469: ATM inhibitor 55933 abolishes the radiation resistance of CD44+/CD24−or low subsets of breast cancer cells
Authors: Hong Yin, Jonathan Glass
Journal: Cancer Research (2011)

Abstract CD44+/CD24− subsets of breast cancer cells are enriched in cancer stem-like cells (CSC) and are radiation resistant. We isolated ESA+/CD44+/CD24− or low subsets by flow cytometry from MCF7, MDA-MB231, and HCC1937 breast cancer cell lines and primary cultures of breast cancer cells from two patients. The isolated CD44+/CD24−orlow subsets showed a 50 to 70% increase of sphere formation by in suspension culture in serum-free medium compared to CD44−/CD24high subsets. CD44+/CD24−or low subsets of the cell lines and primary cultures showed a significantly increased radiation resistance compared to the CD44−/CD24high cell line subsets or the unsorted primary cells with &amp;gt; 40% increased survival at 2Gy. Post-radiation survival showed that the radiation resistance was dependent on the CD44+/CD24 −or low cell phenotype. The correlation of CD44/CD24 status with radiation resistance was demonstrated by culture of the subsets. After 2 weeks of culture the following transitions occurred: 1) CD44+/CD24−or low cells changed to CD44−/CD24high cells (MCF-7 and HCC1937) or to CD44+/CD24+ (MDA-MB-231); and 2) CD44−/CD24 high (MCF-7 and HCC1937) and CD44+/CD24+ (MDA-MB-231) cells became CD44+/CD24−or low cells. Cells that lost the CD44+/CD24 −or low phenotype were no longer radiation resistant. The mechanisms of increased radiation resistance in CD44+/CD24−orlow subsets was not a function of non-homologous end joining (NHEJ) as there was no in vivo and in vitro change in NHEJ activity in the resistant cells nor altered expression of the NHEJ related proteins Ku 80, Ku70, PARP-1, and DNA-PK. However, there were differences ATM activity between CD44+/CD24−or low and the CD44−/CD24 high (MCF-7 and primary breast cancer) or CD44+/CD24+ (MDA-MB-231) subsets. Depending on the cell type and radiation dose, ATM phsophorylation increased by 2-5 fold in radiated CD44+/CD24−or low subsets of breast cancer cell lines and primary cultured breast cancer cells compared to the control subsets. The ATM inhibitor 55933 in a dose-dependent manner increased radiation sensitivity of all subsets including significantly abolishing the radiation resistance of CD44+/CD24−or low subsets of cells. After 2Gy of radiation, the survival fractions of cells treated with10μM of 55933 were 8-10 fold lower than that of untreated cells and were now similar between CD44+/CD24−or low subsets and control subsets for the cell lines and primary cultures. In conclusion, ATM is a candidate target for eliminating the radiation resistance of CSC's of breast cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2469. doi:10.1158/1538-7445.AM2011-2469
US healthcare reform is in the dustbin
Authors: Douglas Kamerow
Journal: BMJ (2010)

Can one state’s senatorial election really scuttle the whole thing?

The initial conventional wisdom about the race for the late Edward Kennedy’s US Senate seat was that there was no chance that the Republican candidate, Scott Brown, could defeat the Massachusetts attorney general, the Democrat Martha Coakley. It’s a heavily Democratic state, it was Kennedy’s seat for almost 50 years, and it was crucial for the Democrats to maintain their 60-40 advantage in the US Senate.

As everyone now knows, this conventional wisdom was wrong. In the fading days of the campaign Coakley’s large lead evaporated and Brown won handily, with 53% of the vote.

The second bit of conventional wisdom about this race, explaining the amazing upset, was that it was a vote against President Obama’s policies, especially healthcare reform. A perfect storm, pundits said: a weak Democratic candidate, a poorly run campaign, and voters impatient for economic recovery, all in a state that already had passed its own universal health coverage law and thus “didn’t need” a new federal law.

As everyone may not know, however, the answer to …
Case 38-1977
Authors: Richard C. Cabot, Robert E. Scully, James J. Galdabini, Betty U. McNeely, Sheldon D. Kaufman, John C. Long
Journal: New England Journal of Medicine (1977)

Presentation of CaseA 36-year-old woman was admitted to the hospital because of a mass in the parotid gland.She was well until one year previously, when a hard lump was felt at the left mandibular angle. She was evaluated at a hospital in Georgia, where she was said to have a benign swelling. A routine x-ray film of the chest, taken three months earlier, was reported to be normal. During the next few months the mass became smaller but did not disappear. Seven months before admission she returned to Boston after a stay of seven years in Georgia. She . . .
Sterility after Vasectomy
Authors: C. Maddox, V. Patel
Journal: BMJ (1973)

We consider that a self-administered questionnaire can be used to detect depression, although a single direct question such as we asked would not be very sensi- tive.However, it is noteworthy that 29 °/, of the replies to the question were positive.There was no excess of positive replies in patients taking methyldopa compared with patients taking other drugs.Patients who became severely depressed on any treatment regimen would have their treatment altered.These patients, however, represent only a stnall proportion of the patients studied.Drs. R. P. Snaith and M. McCoubrie (personal communication) have given the Wakefield Self-Assessment Depression In- ventory to 134 patients receiving me-thyldopa, and have also failed to demonstrate an increase in depression while taking this drug.-Weare, etc.,
Relations Between Psychiatry and Psychology
Authors: Sidney Berman
Journal: JAMA (1966)

<h3>To the Editor:—</h3> The issue of diagnosis and therapy is of concern to physicians specializing in psychiatry, for this is the primary responsibility of a licensed practitioner of medicine. Therefore I would question the medical ethics of any physician who refers a patient to a clinical psychologist in private practice for diagnosis or unsupervised treatment. There are a number of state medical societies and district branches of the American Psychiatric Association concerned with the expansion of the nonmedical treatment of the mentally ill. Herman A. Dickel, MD (<b>195</b>:365, 1966) speaks in direct opposition to the overwhelming majority of physicians who specialize in psychiatry. It is fantastic to say that only recently have "physicians realized that 'psychosomatic' illness is one of their responsibilities...." I have been teaching this in elaborate programs in a medical school for the past 25 years. I fear that Dr. Dickel is the spokesman for the
A deadly web
Authors: Alexandra M Nanzer, Simon Jordan, Simon Padley, Mark Griffiths, Matthew Hind
Journal: Thorax (2014)

An 18-year-old patient with metabolic myopathy due to long-chain fatty acid coenzyme A dehydrogenase-deficiency1 ,2 presented with worsening positional dyspnoea. He had required invasive ventilation via tracheostomy 6 months earlier. Examination revealed respiratory distress, a prolonged inspiratory phase and …
Disparate Innate Immune Responses to Persistent and Acute<i>Chlamydia pneumoniae</i>Infection in Chronic Obstructive Pulmonary Disease
Authors: Daniel Droemann, Jan Rupp, Torsten Goldmann, Ulrike Uhlig, Detlev Branscheid, Ekkehard Vollmer, Peter Kujath, Peter Zabel, Klaus Dalhoff
Journal: American Journal of Respiratory and Critical Care Medicine (2007)

Chlamydia pneumoniae (Cpn) infection may play a role in the pathogenesis of chronic obstructive pulmonary disease (COPD). Few data are available comparing persistent and acute infection of this pathogen in the human respiratory tract.To study Cpn-induced innate immune responses in lung tissue from patients with COPD and control subjects ex vivo and in vitro.Cpn detection was done by nested polymerase chain reaction, in situ hybridization, and immunohistochemistry ex vivo in unstimulated tissue and in vitro using an acute Cpn infection model. As main endpoints for the assessment of early cellular responses, nuclear factor (NF)-kappaB activation and CXC chemokine ligand (CXCL)-8 expression were evaluated. The role of Toll-like receptors (TLRs) as recognition molecules in Cpn-induced innate responses was tested by blocking experiments.Fifteen percent of patients with COPD were chronically infected with Cpn in contrast to 0% of control subjects (p < 0.05). There were no differences in CXCL-8 and NF-kappaB expression between infected and noninfected COPD tissue ex vivo. In contrast, acute in vitro infection induced an intense innate immune response including up-regulation of TLR2. Blocking experiments demonstrated the predominant role of TLR2 in induction of the early immune response, whereas no influence on chlamydial infection rates was observed.Acute in vitro infection of human lung tissue with Cpn elicited a marked innate response via TLR2, whereas chronic chlamydial infection in patients with COPD was not associated with enhanced cellular activation. These findings suggest different roles of Cpn during acute and chronic stages of pulmonary infection.
Physician Performance Measurement
Authors: Joseph P. Drozda
Journal: JAMA Internal Medicine (2013)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Internal Medicine HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Internal Medicine journal
Aminophylline Improves Diaphragmatic Contractility
Authors: Michel Aubier, André De Troyer, Michael Sampson, Peter T. Macklem, Charis Roussos
Journal: New England Journal of Medicine (1981)

In an attempt to explain the clinical efficacy of aminophylline, we studied its effect on diaphragmatic function in eight normal subjects. The relation between the electrical activity of the diaphragm and the pressure generated by the diaphragm was assessed during voluntary contractions before and after aminophylline infusion. Aminophylline shifted the electrical activity/pressure curve to the left; the pressure at a given electrical activity increased an average of 15 per cent (P less than 0.001). In four subjects, pressure was also measured during stimulation of the phrenic nerve at various frequencies before and after diaphragmatic fatigue was produced by resistive breathing, with and without aminophylline infusion. Pressure increased after fatigue at all stimulation frequencies with aminophylline, as compared with the pressure after identical fatigue runs at the same stimulation frequencies without aminophylline. The mean plasma aminophylline concentration associated with these responses was 13 +/- 0.9 mg per liter. We conclude that aminophylline improves the diaphragm's contractility and renders it less susceptible to fatigue.
Quality Control in Blood Banking
Authors: Frances K. Widmann
Journal: JAMA (1975)

The blood banker seeking a quality control program sometimes feels like Goldilocks at the bears' home: some suggested programs are Too Big, others are clearly Too Small. Myhre's handbook outlines procedures and suggests combinations that can be Just Right for each individual laboratory. Sensibly cautioning against spending more time on quality control than on getting things done, the author discusses which areas need continuous surveillance, which procedures are particularly prone to subtle errors, and which operations or equipment can safely be spot-checked. Ingenious suggestions are given for such problems as standardizing what "1+ " or "3+ " means to many different workers, determining the completeness of washing for antiglobulin tests, and evaluating the degree to which albumin or enzymes enhance agglutination. Although a brief chapter considers automated techniques, the manual is clearly people-oriented; sympathy for human frailty is combined with determination to protect patients, laboratory workers, and administrators from inevitable errors of
Combination of Adjuvant Hormonal and Radiation Therapy Significantly Prolongs Survival of Patients With pT2–4 pN+ Prostate Cancer: Results of a Matched Analysis
Authors: Alberto Briganti, R. Jeffrey Karnes, Luigi Filippo Da Pozzo, Cesare Cozzarini, Umberto Capitanio, Andrea Gallina, Nazareno Suardi, Marco Bianchi, Manuela Tutolo, Andrea Salonia, Nadia Di Muzio, Patrizio Rigatti, Francesco Montorsi, Michael Blute
Journal: European Urology (2011)
Risnk of Myocardial Infarction With Elective Vascular Surgery
Authors: Jerry Goldstone
Journal: JAMA (1978)

<h3>To the Editor.—</h3> The recent article by Steen et al (239:2566, 1978) is timely and an excellent follow-up to the earlier report by Tarhan et al (220:1451, 1972). All surgeons are aware of the risk of myocardial infarction with surgery; however, there is little information available on the risk of primary myocardial infarction with elective surgical procedures. Interestingly enough, the data presented by the authors substantiate the data we previously published regarding the risk of primary myocardial infarction postoperatively in patients undergoing elective aortobilateral femoral bypass grafting.<sup>1</sup>The incidence in 180 patients was 3%. Analysis of the incidence of associated risk factors, including preexisting hypertension, diabetes mellitus, abnormal ECG, angina pectoris, and history of prior myocardial infarction more than six months before surgery, demonstrated that the only one showing a statistically significant correlation (x<sup>2</sup>, corrected for continuity) with the incidence of postoperative myocardial infarction was preoperative hypertension (P&lt;.01).
Upper gastrointestinal endoscopy in the elderly.
Authors: S P Lockhart, P M Schofield, R J Gribble, J H Baron
Journal: BMJ (1985)

Nevertheless, antibody activity against insulin and penicillin has been associated with IgD,5 which led us to wonder whether this immuno- globulin molecule represents a septor for the antigenic dete ts in dapsone, although we could not confirm this in our patient.1 Carter A, Tatarsky I.The physiopathological significance of benign monoclonal gammopathy: a study of 64 cases.
OCCIPITO-POSTERIOR PRESENTATION
Authors: G. Geddes
Journal: BMJ (1906)

perature was 1010 F., and the piulse 110.A fortnighlt before this she noticed a lump oIn her left side, whlich slhe leclared moved about and chlanged its position fr-om time to time.The lump, about the size of the fist, was hard, smooth, very tender oni lhandling, dull oni percussioni, and tympanitic all round, with a well-marked sulcus between it and tlle pregnant uterus, wllich it had puslhed to tlhe * Part of an address to th6 Leeds UniversitV Medical Society on November 28th, 1903.Poitions, deating witth the Leeds Infirmary and its medical staff of the past, as well as with that of Moorfields Ophtlhalmic Hlospital in thlose days, are omitted.
QT interval dispersion analysis in patients with acute myocardial infarction
Authors: Bernadino Tranchesi, Roberto Kalil, Neuza Lopes, Cicero de Albuquerque, Carlos Rochitte, Beatriz Ferreira, Ricardo Lima, Cesar Gruppi, Giovanni Bellotti, Fulvio Pileggi
Journal: Journal of the American College of Cardiology (1996)
Effect of Angiotensin Infusion on Regional Blood Flow and Regional Vascular Resistance in the Rat
Authors: Morris J. Mandel, Leo A. Sapirstein
Journal: Circulation Research (1962)

The effects of infusions of angiotensin of 0.05 to 0.5 µg./Kg./min. on the cardiac output, arterial blood pressure, and regional blood flow in the rat have been measured. Angiotensin increases the blood pressure without increasing the cardiac output; the total peripheral resistance is increased. Territorial resistances show varying degrees of increase, ranging from the cerebral vascular resistance, which is reduced or unchanged, to the renal vascular resistance, which shows a 100 to 200 per cent increase. The smallest increases in resistance in other organs are seen in the heart and lungs, which are unchanged, and the spleen and adrenal, which show minimal elevations. Larger changes are seen in the carcass, gut, and skin. The overall appearance of the changes in the distribution of blood flow in the animal infused with angiotensin suggests that blood flow is diverted from the kidneys to those organs most concerned with the immediate response of the organism to an emergency.
BRONCHIECTASIS WITH UNUSUAL COMPLICATIONS
Authors: I. J. Davies
Journal: BMJ (1923)

__19231_ _I____MEDI JoumxaSRe-amputation May 5th, 1916.The fibula was excised and flaps were dissected from the anterior and posterior aspects of the amputation stump; the anterior and posterior tibial nerves were cut short and the flaps brought together with as little tension as possible.Four months later the stump was soundly healed.He was fitted with an artifloial limb in.December, 1916, and the fol- lowing May he was able to walk and stand with ease and comfort and could play a round of golf over a course of eighteen holes.In this case without removal of the fibula it would have been impossible to cover the ends of the stump witlh a satisfactory skin flap.Removal of the fibula enabled a satisfactory stump to be obtained below the knee-joinit, and a limb could be fitted which gave the patient full use of the kilee.CASE II.-Gunitshot Wound of Right Leg: Amiipuitationt belowv the Knee with Excisiont of the 'ibulta.Result, S'erviceaible Stumip. A. G., a fish curer, aged 25, was wounded in the right leg in May, 1916.The leg was'amputated by the guillotine method, and he was transferred to Enigland.The stump below the knee measured 2& inches, and its extremity was covered by a layer of healthy granulation.Three months after the primary amputation a second amputation was done: the fibula was excised, the external and posterior popliteal nerve dissected out and cut as short as possible, and skin flaps were freed and brought over the stump with as little tensioni as possible.In six months' time the stump was completely healed and ready for fitting with an artificial limb.The patienit was given his first limb in January, 1917, and his seconid limb in September, 1919.He lhas returned to his previous
Human immunodeficiency virus-1 infection of macrophages in vitro neither induces tumor necrosis factor (TNF)/cachectin gene expression nor alters TNF/cachectin induction by lipopolysaccharide.
Authors: J R Munis, D D Richman, R S Kornbluth
Journal: Journal of Clinical Investigation (1990)

The synthesis of tumor necrosis factor (TNF)/cachectin was assessed in primary monocyte-derived macrophage (MDM) cultures after in vitro infection with a macrophage-tropic strain of HIV-1 (HTLV-IIIBa-L/85). Productive and cytopathic infections in MDM cultures were established using a high multiplicity of infection (m.o.i. = 3) under conditions that minimized endotoxin contamination. Culture supernatants were tested for TNF/cachectin activity by L929 cell cytotoxicity assay, and TNF/cachectin mRNA was assessed by a sensitive PCR amplification technique that could detect between 1 and 10 cells fully activated for TNF/cachectin expression. Unstimulated MDM cultures produced no detectable levels of TNF/cachectin activity or mRNA, consistent with previous demonstrations that production of this cytokine by macrophages is an inducible and not a constitutive event. HIV-1 infection failed to induce detectable TNF/cachectin activity or mRNA in these unstimulated cultures. In addition, the responsiveness of macrophages to lipopolysaccharide (LPS) induction of TNF/cachectin production was assessed in dose-response and kinetic experiments. No differences between infected and uninfected cultures were discernable. These results demonstrate that productive and cytopathic infection with a macrophage-tropic strain of HIV-1 does not alter the regulation of TNF/cachectin expression in macrophages.
Interleukin-10 Signaling in Regulatory T Cells Is Required for Suppression of Th17 Cell-Mediated Inflammation
Authors: Ashutosh Chaudhry, Robert M. Samstein, Piper Treuting, Yuqiong Liang, Marina C. Pils, Jan-Michael Heinrich, Robert S. Jack, F. Thomas Wunderlich, Jens C. Brüning, Werner Müller, Alexander Y. Rudensky
Journal: Immunity (2011)
Synovial Hemangioma as a Cause of Recurrent Knee Effusions
Authors: Marc A. Linson
Journal: JAMA (1979)

SYNOVIAL hemangioma is an unusual cause of knee effusions, but must be considered in the individual who has recurrent nontraumatic knee effusions. The diagnosis is best made by eliciting a characteristic history and physical examination, finding grossly bloody fluid on joint aspiration, and pursuing with arthrography or arthroscopy and then arthrotomy. The case described here was of 20 years' duration. <h3>Report of a Case</h3> A 22-year-old man was seen with the latest in a long series of recurrent nontraumatic left knee effusions. His first effusion had occurred at age 2 years when a swollen, painful left knee had suddenly developed without inciting trauma. On examination, he had been found to have a temperature of 39 °C, a moderate effusion of his left knee, and marked tenderness, especially over the medial femoral condyle. Roentgenograms had been normal; WBC count, 11,400/cu mm, with a normal differential cell count; and erythrocyte sedimentation rate,
Needle Track Implantation of Tumor After Percutaneous Lung Biopsy
Authors: HARVEY WOLINSKY
Journal: Annals of Internal Medicine (1969)

The second documented case of needle track implantation of tumor after percutaneous biopsy of an adenocarcinoma of the lung is presented. This complication is so rare that a return to the prior skepticism that surrounded this procedure is not justified. On the other hand, the currently prevailing atmosphere of widespread enthusiasm should not lead to adoption of the use of percutaneous needle biopsy in clearly operable patients in view of the real hazard of local dissemination of tumor.