tbuckley/cabot-search huggingface dataset:
Predictive Value of Microvolt T-Wave Alternans in Patients With Left Ventricular Dysfunction
Authors: Daniel J. Cantillon, Kenneth M. Stein, Steven M. Markowitz, Suneet Mittal, Bindi K. Shah, Daniel P. Morin, Eran S. Zacks, Matthew Janik, Shaun Ageno, Andreas C. Mauer, Bruce B. Lerman, Sei Iwai
Journal: Journal of the American College of Cardiology (2007)
Protection of Immuno-Compromised Mice from Lethal Infection of Klebsiella Pneumonia by rAAV2-BPI23-Fcγ1 Gene Transfer
Authors: Jing Li, Qingli Kong, Zhe Lv, Yuanzhi Guan, Yong Qiu, Chen Li, Mingjie Sun, Zhenlong Liu, Yunqing An
Journal: Cellular and Molecular Immunology (2008)
Cell surface expression of murine, rat, and human Fc receptors by Xenopus oocytes.
Authors: E Pure, A D Luster, J C Unkeless
Journal: The Journal of Experimental Medicine (1984)

We report that Xenopus laevis oocytes can efficiently translate and insert heterologous membrane receptors into the oocyte plasma membrane, where they can be detected by the binding of either monoclonal antibodies or ligands. Thus, oocytes injected with mRNA from the mouse J774 macrophage-like cell line, the rat RBL-1 basophilic leukemia, and the U937 promonocyte cell line, bound 2.4G2 Fab, rat IgE, and mouse IgG2a, respectively. The increase in the high avidity Fc gamma R observed after gamma-interferon induction of U937 cells was also observed after injection of mRNA from gamma-interferon-induced U937 cells into oocytes. This suggests either much greater message stability or a greater rate of transcription of Fc gamma Rhi mRNA in the gamma-interferon-induced cells. The assay affords a sensitive method for the detection of rare mRNA species that code for plasma membrane proteins.
Seroconversion to hepatitis C virus alternate reading frame protein during acute infection #
Authors: Yoann Morice, Maxime Ratinier, Ahmed Miladi, Stéphane Chevaliez, Georgios Germanidis, Heiner Wedemeyer, Syria Laperche, Jean-Pierre Lavergne, Jean-Michel Pawlotsky
Journal: Hepatology (2009)

The existence of hepatitis C virus (HCV) proteins encoded by alternate reading frames overlapping the core-encoding region has been suggested. Several mechanisms of production have been postulated, and the functions of these proteins in the HCV life cycle remain unknown. We analyzed cases of seroconversion to an alternate reading frame protein in a group of 17 patients infected by one of the two HCV genotype 1b strains during an outbreak in a hemodialysis unit. Three patients seroconverted, and antibodies were transiently detected in another patient. Three of these patients were infected by one of the two HCV strains, whereas the strain infecting the remaining patient could not be identified. Quasispecies sequence analysis of the core-coding region showed no differences in the core or +1 reading frame sequences that could explain alternate reading frame protein seroconversion in some but not all of the patients infected by one of the HCV strains, and no such difference was found between the two strains. Because differences in the structure of RNA elements could play a role in frameshift events, we conducted a predictive analysis of RNA folding. No difference was found between the patients who did and did not seroconvert to alternate reading frame protein.Our findings prove that alternate reading frame proteins can be produced during acute HCV infection. However, seroconversion does not occur in all patients for unknown reasons. Alternate reading frame protein could be generated by minority quasispecies variants or variants that occur transiently.
Novel multimodality biologic response modifier therapy, including bexarotene and long-wave ultraviolet A for a patient with refractory stage IVa cutaneous T-cell lymphoma
Authors: Michael Shapiro, Alain H. Rook, Michael S. Lehrer, Jacqueline M. Junkins-Hopkins, Lars E. French, Carmela C. Vittorio
Journal: Journal of the American Academy of Dermatology (2002)
Heat stroke in a "run for fun".
Authors: T J Bassler
Journal: BMJ (1979)

The routine use of PET/CT fusion imaging in a large oncology practice has led to the realization that <sup>18</sup>F-FDG uptake into normal fat and muscle can be a common source of potentially misleading false-positive PET imaging in the neck, thorax, and abdomen. The goal of this study was to characterize this normal variant of <sup>18</sup>F-FDG uptake in terms of incidence and characteristic extent. <b>Methods:</b> All body scans done on our PET/CT scanners in July and August 2002 were retrospectively reviewed. All cases in which increased <sup>18</sup>F-FDG uptake in the neck was not localized to lymph nodes or other obvious anatomic sites on the CT scans were included in this study. Sites of any unexplained <sup>18</sup>F-FDG uptake in the rest of the body were also recorded. <b>Results:</b> A total of 863 PET scans (476 males, 387 females; age, 2–88 y; mean age, 57 y) were reviewed. The following distinctive patterns of nonpathologic <sup>18</sup>F-FDG activity were observed: (a) neck fat, 20 patients (2.3%); (b) paravertebral uptake, 12 patients (1.4%); (c) perinephric fat, 7 patients (0.8%); (d) mediastinal fat, 8 patients (0.9%); (e) normal musculature, 12 patients (1.4%). Patients showing paravertebral uptake, perinephric fat, and mediastinal fat were all associated with the neck fat pattern, singly or in combination. On the other hand, patients showing the normal musculature pattern did not show any of the other uptake. In this analysis, the incidence of <sup>18</sup>F-FDG uptake in sites other than the neck is restricted to the patient population with neck fat uptake and may be an underestimation of the true incidence. Neck fat is found predominantly in females, whereas normal musculature is usually seen in males (<i>P</i> &lt; 0.01, Fisher exact test). Neck fat is also seen significantly more in the pediatric population (4/26 = 15%) than in the adult population (16/837 = 1.9%) (<i>P</i> &lt; 0.01, Fisher exact test). <b>Conclusion:</b> Increased <sup>18</sup>F-FDG uptake is sometimes seen in individual muscles and in adipose tissue in the neck and shoulder region, axillae, mediastinum, and perinephric regions. There is also associated <sup>18</sup>F-FDG uptake in the intercostal spaces in the paravertebral regions. <sup>18</sup>F-FDG uptake in neck fat is more commonly seen in female patients and the pediatric population. The pattern of uptake as well as the age and sex distribution suggest that the <sup>18</sup>F-FDG in fat is in the brown adipose tissue. It is important to recognize this uptake pattern to avoid false interpretation of this benign normal variant as a malignant finding on <sup>18</sup>F-FDG PET scans.
Hemibody Uptake on Bone Scintigraphy in Polyostotic Fibrous Dysplasia
Authors: Boris Rudenko, Sebastien Klingelschmitt, Sebastien Aubry, Moussa Bamba, Hatem Boulahdour
Journal: Clinical Nuclear Medicine (2003)

Increased uptake of bone radiopharmaceuticals is usually observed on bone scintigraphy in fibrous dysplasia. In accordance with the literature, most cases were monostotic, leading to only 1 focus of increased uptake. Approximately 15% of cases are polyostotic, leading to multifocal areas of increased uptake. This case demonstrates unusual hemibody uptake on bone scintigraphy.
Leukemia treatment in severe combined immunodeficiency mice by antisense oligodeoxynucleotides targeting cooperating oncogenes.
Authors: T Skorski, M Nieborowska-Skorska, K Campbell, R V Iozzo, G Zon, Z Darzynkiewicz, B Calabretta
Journal: The Journal of Experimental Medicine (1995)

Transformation of hematopoietic cells by the p210bcr/abl tyrosine kinase appears to require the expression of a functional MYC protein, suggesting that simultaneous targeting of BCR-ABL and c-myc might be a rational strategy for attempting treatment of Phil-adelphia leukemia. To test this hypothesis, severe combined immunodeficiency mice injected with Philadelphia leukemic cells were treated systemically with equal doses of bcr-abl or c-myc antisense oligodeoxynucleotides (ODNs) or with both ODNs in combination. Compared with the mice treated with individual agents, the disease process was much slower in the group treated with both ODNs, as revealed by flow cytometry, clonogenic assay, and reverse transcriptase-polymerase chain reaction analysis to detect leukemic cells in mouse tissue cell suspensions, and by enumeration of liver metastases. The retardation of the disease process was positively correlated with a markedly increased survival of leukemic mice treated with both ODNs. These data demonstrate the therapeutic potential of targeting multiple cooperating oncogenes.
Microfilament-dependent activation of Na+/K+/2Cl- cotransport by cAMP in intestinal epithelial monolayers.
Authors: J B Matthews, C S Awtrey, J L Madara
Journal: Journal of Clinical Investigation (1992)

cAMP-mediated stimulation of Cl- secretion in the human intestinal cell line T84 is accompanied by significant remodeling of F-actin, and both the secretory and cytoskeletal responses may be largely ablated by previous cell loading with phalloidin derivatives, reagents that prevent dynamic reordering of microfilaments (1991. J. Clin. Invest. 87:1903-1909). In this study, we examined the effect of phalloidin loading on the cAMP-elicited activity of the individual membrane-associated transport proteins involved in electrogenic Cl- secretion. Efflux of 125I and 86Rb was used to assay forskolin-stimulated Cl- and K+ conductances, respectively, and no inhibitory effect of phalloidin could be detected. Na+/K(+)-ATPase pump activity, assessed as bumetanide-insensitive 86Rb uptake and the ability of monolayers to generate a Na+ absorptive current in response to apical addition of a Na+ ionophore, was not different between control and phalloidin-loaded monolayers. Forskolin was found to stimulate Na+/K+/2Cl- cotransport (bumetanide-sensitive 86Rb uptake) in time-dependent fashion. In the absence of any agonist, cotransporter activity was markedly decreased in phalloidin-loaded monolayers. Furthermore, under phalloidin-loaded conditions, the forskolin-elicited increase in bumetanide-sensitive 86Rb uptake was markedly attenuated. These findings suggest that cAMP-induced activity of Cl- channels, K+ channels, and the Na+/K(+)-ATPase are not influenced by F-actin stabilization. However, cAMP-induced activation of the Na+/K+/2Cl- cotransporter appears to be microfilament-dependent, and ablation of this event is likely to account for the inhibition of cAMP-elicited Cl- secretion seen in the phalloidin-loaded state. Such findings suggest that Na+/K+/2Cl- cotransporter is functionally linked to the cytoskeleton and is a regulated site of cAMP-elicited electrogenic Cl- secretion.
Hepatic cytochrome P450 is directly inactivated by nitric oxide, not by inflammatory cytokines, in the early phase of endotoxemia
Authors: Shigekazu Takemura, Yukiko Minamiyama, Susumu Imaoka, Yoshihiko Funae, Kazuhiro Hirohashi, Masayasu Inoue, Hiroaki Kinoshita
Journal: Journal of Hepatology (1999)
Must be trained in geriatric medicine
Authors: D. O'Neill, K. Andersen-Ranberg, A. Cherubini, T. Strandberg, J. Petermans, J.-P. Michel
Journal: BMJ (2012)

It is important to focus on how we deliver the most acute stages of hospital care when acute medical admissions are likely to comprise older patients with multimorbidity, frailty, and polypharmacy.1 A recent Cochrane review showed that the acute geriatric medicine model was extremely efficient, with substantial reductions in death, disability, and institutionalisation compared with …
Invited commentary on this month's Controversy articles: From P G Hugenholtz
Authors: P G Hugeholtz
Journal: Cardiovascular Research (1994)

Journal Article Invited commentary on this month's Controversy articles: From P G Hugenholtz Get access P G Hugeholtz P G Hugeholtz Erasmus University, Rotterdam, The Netherlands. Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 28, Issue 4, April 1994, Pages 576–577, https://doi.org/10.1093/cvr/28.4.576 Published: 01 April 1994
TCT-270 Non-Cocktail Strategy for Transradial Procedures. A Sub-Analysis of an International Multicenter Registry
Authors: Pablo E. Kantor, Surya Dharma, Andres Dini, Ian C. Gilchrist, Alejandro Goldsmit, Sasko Kedev, Ferdinand Kiemeneij, Tak Kwan, Fabricio Monacci, Tejas Patel, Gustavo Tamashiro, Leon Valdivieso, Bernhard Westerberg
Journal: Journal of the American College of Cardiology (2013)
Cost of Drugs in Australia's Pharmaceutical Benefit Scheme
Authors: Rebecca A. Derby
Journal: JAMA (1997)

<h3>To the Editor.</h3> —The article by Dr Peabody and colleagues<sup>1</sup>on the Australian health care delivery system and its relevance to the United States offered much interesting information, but their statement that pharmaceutical costs per capita in Australia are 20% greater than in the United States may be misleading to those trying to gain an understanding of Australia's Pharmaceutical Benefit Scheme (PBS). If accurate, this figure demonstrates more about lack of access to medicines in the United States than expense of drugs with the PBS. It has been suggested that prescription drug use rates are lower in the United States than Australia. This is primarily due to 2 factors: first, lack of prescription drug insurance coverage in the United States and, second, the higher prices that manufacturers charge in the United States compared with the rest of the industrialized world. Manufacturers' prices for the same prescription drug are generally
Transfusion Practices in the 1990s
Authors: J E Menitove
Journal: Annual Review of Medicine (1991)

Transfusion practice in the 1990s is expected to continue the trend established in the 1980s toward assuring appropriate use of transfusion therapy. Autologous blood collection is expected to increase further, and pharmacologic agents that promote hemostasis or cell production will be integrated into ongoing efforts to reduce homologous blood usage. Although the risk of transfusion-transmitted diseases should continue to decrease, procedures to inactivate viruses in cellular blood components will be evaluated and artificial oxygen-carrying components may become available.
Incidence of Asymptomatic Intracranial Embolic Events After Pulmonary Vein Isolation
Authors: Claudia Herrera Siklódy, Thomas Deneke, Mélèze Hocini, Heiko Lehrmann, Dong-In Shin, Shinsuke Miyazaki, Susanne Henschke, Peter Fluegel, Jochen Schiebeling-Römer, Paul M. Bansmann, Thomas Bourdias, Vincent Dousset, Michel Haïssaguerre, Thomas Arentz
Journal: Journal of the American College of Cardiology (2011)
Abstract 1197: The role of mitochondrial permeability transition pore complex proteins VDAC1, ANT, and cyclophilin D in prednisolone-induced apoptosis in B-Lineage acute lymphoblastic leukemia (ALL)
Authors: Nan Jiang, Grace Shi Min Koh, Shirley Kow Yin Kham, Fook Tim Chew, Allen Eng Juh Yeoh
Journal: Cancer Research (2010)

Abstract The loss of mitochondrial membrane permeability is central to apoptosis. The mitochondrial permeability transition pore (PTP) complex, comprising of at least voltage-dependent anion channel 1 (VDAC1), adenine nucleotide transporter (ANT) and cyclophilin D (CyD), mediates the permeability of the mitochondrial membranes. We investigated the involvement of VDAC1, ANT and CyD in prednisolone (PRED)-induced apoptosis in 2 ALL cell lines: Sup-B15 (sensitive) and REH (resistant). After 24h exposure to PRED (10.0µg/ml), VDAC1 protein was consistently upregulated only in PRED-sensitive cell line, but remained unchanged in the resistant one. Co-administration of non-specific VDAC1 inhibitor, 4, 4′-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) with PRED, blocked PRED-induced apoptosis and caspase-3 activity only in sensitive cell line. Surprisingly, complete VDAC1 silencing using siRNA (Dharmacon) failed to abrogate PRED-induced apoptosis, suggesting that VDAC1 is not critical. We next investigated the other members of the PTP complex i.e., ANT and CyD. Co-administration of PRED and bongkrekic acid (BA) a specific inhibitor of ANT, also resulted in a dose-dependent inhibition of apoptosis and the caspase-3 activity. The combination of DIDS and BA, even at reduced doses by 60% and 96% respectively, is highly synergistic in inhibiting PRED-induced apoptosis and surprisingly reduced VDAC1 upregulation in PRED-induced apoptosis, unlike each agent alone. PRED did not alter the level of CyD protein and administration of specific CyD inhibitor, cyclosporine A (CsA), did not affect PRED-induced apoptosis. We conclude that ANT protein is central in the mitochondrial PTP mediated apoptosis during PRED treatment of ALL; VDAC1 and CyD are dispensable. ANT is upstream to VDAC1 and it upregulates VDAC1 during PTP activation in PRED-induced apoptosis. Our investigation provides the basis for looking at the role of mitochondrial PTP complex proteins especially ANT in PRED treatment in childhood ALL. This may provide new avenues to reverse resistance to therapy in patients with relapsed ALL. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1197.
Thiazide-lnduced Hypomagnesemia
Authors: Michael J. Moore
Journal: JAMA (1978)

<h3>To the Editor.—</h3> A recent issue had an interesting article entitled "Thiazide-Induced Hypokalemia: Association With Acute Myocardial Infarction and Ventricular Fibrillation" (239:43, 1978). I saw nowhere in it references to serum magnesium in such patients or discussions of the importance of it. This has been written about for several years,<sup>1,2</sup>but the information does not appear to have had wide dissemination. Burch and Giles<sup>3</sup>have recently reviewed this topic, and Flink and associates<sup>4-6</sup>have done extensive research and writing on magnesium deficiency and toxicity. Diuretics can cause both hypokalemia and hypomagnesemia, and if hypomagnesemia is present, it is essentially impossible to correct the hypokalemia. As a matter of fact, it is a routine order for the coronary care unit nurses in our hospital to obtain the serum magnesium level of new patients who have been taking diuretics for any appreciable length of time. This applies to patients
Misclassification Problems in Diagnosis-Related Groups
Authors: Susan D. Horn, Roger A. Horn, Phoebe D. Sharkey, Robert J. Beall, John S. Hoff, Beryl J. Rosenstein
Journal: New England Journal of Medicine (1986)

Abstract Under the Medicare prospective payment system, which is based on diagnosis-related groups, patients with certain diseases may be inappropriately classified. To study this problem using cystic fibrosis as an example, we examined discharge-abstract data from 14 cystic fibrosis centers in a comparison of resource-use requirements by patients with cystic fibrosis and other patients in the same diagnosis-related group. There were 1763 patients with cystic fibrosis and 25,628 other patients in the 87 diagnosis-related groups that contained at least one patient with cystic fibrosis. For the eight diagnosis-related groups in which patients with cystic fibrosis were classified most often, the average length of stay of patients with cystic fibrosis was 14.9 days, as compared with an average of 8.3 days for the other patients (P<0.001). For three hospitals, we were able to convert charges to costs. The average cost of treating patients with cystic fibrosis was $7,262, as compared with $2,908 for all other patients in the same diagnosis-related group (P<0.001). The ratio between the costs of treating patients with cystic fibrosis and other patients (2.5) was greater than the ratio between the lengths of stay for the two groups (1.8), reflecting the more intense use of resources by the patients with cystic fibrosis. A possible solution to the problem of misclassification is to define one or more new diagnosis-related groups for cases of cystic fibrosis or determine a new location within the diagnosis-related group system so that patients with cystic fibrosis can be classified with patients who use similar amounts of resources. (N Engl J Med 1986; 314:484–7.)
Patient and Specialist — Future of General Practice — Fifi, the Hippopotamus
Authors: John Lister
Journal: New England Journal of Medicine (1962)

THE professional reputation of seven leading plastic surgeons was vindicated when the Disciplinary Committee of the General Medical Council concluded that none of the surgeons were guilty of infamous conduct, in a professional respect, for having advertised for the purpose of obtaining patients.The case had arisen as the result of two articles, which appeared in popular women's magazines, on the benefits of plastic surgery and recommended application to the British Association of Plastic Surgeons for the names of specialists. In connection with the second article, a leaflet on the scope of plastic surgery had been prepared and seen by . . .
A COMPARISON OF THE EFFICIENCY OF SOME METHODS OF OXYGEN ADMINISTRATION
Authors: R. Hilton
Journal: BMJ (1928)

MEDICcL JouURN4A reactioi, in 1 agreemenit with the Sigma, in 1 Ipartial agreement 'witlh the Sigmiia, an(d in 2 partial agreemnent .with the Wassermann reactioni.False positives may tlherefore be reduced to 3 (in 2 of which the readings were N P, P, and D P P respectively).There were 6 apparently false positive Wassermann reactions.In 2 the Kahni agreed; in 2 the Kahn partially agreed; in 1 the Sigma partially *agreed, anid one case was probably syphilis.There were thus 3 " false positives," which gave the following read- ings: + ± (test repeated with the same result), + (test -repeated with the same result), and +.There was 1 apparently false positive Sigma test with which the Kahn agreed.Firom the above it woould appear that whilst there was a high percentage of agr-eement betweeni thte tliree tests the results were slightly, but definitely, in favour of the Kalhn.It is not suggested that this test could or should relplace the Wasserimauii, but it is a very useful additional test, especially i"n cases w-hei'e the diagnosis is in doubt or where the Wassermann reaction gives a + resut.The Kahn test appears to give a definitely greater p'roportion of positives in cases of treate(l syphilis; in other worids,
Use of labelled triolein, vitamin A, and D-xylose in the diagnosis of malabsorption
Authors: K. G. Wormsley
Journal: Gut (1963)

This paper discusses the type of results given by a group of test procedures used in the study of small intestinal dysfunction. While the chemical estimation of faecal fat remains the most valuable criterion for the laboratory detection of malabsorption, the occurrence of abnormal faecal or blood radioactivity also denotes the presence of malabsorption, although some patients with staetorrhoea appear to `absorb9 labelled triolein normally. Analysis of data from the other tests shows that steatorrhoea is not necessarily present in patients who give abnormal results and that the presence of malabsorption does not preclude a normal capacity for handling the test substances. These reservations impair the value of the vitamin A and xylose `tolerance9 tests and serum carotene levels as screening procedures for the determination of malabsorption states. On the other hand, these tests show patterns of results which are useful in differentiating idiopathic from secondary steatorrhoea, since patients with secondary malabsorption often handle one or more of these test substances normally.