tbuckley/cabot-search huggingface dataset:
Abstract 5189: The transcriptional regulation of the long non-coding RNA HOTAIR in ovarian cancer.
Authors: Ali R. Ozes, Dave Miller, Cong Guo, Anurag Bhattrai, Yunlong Liu, Kenneth P. Nephew
Journal: Cancer Research (2013)

Abstract The Polycomb Group (PcG) Repressive Complexes 1 and 2 (PRC1 and PRC2) maintain gene expression patterns by epigenetically modifying chromatin. PRC2 suppresses transcription by catalyzing the trimethylation of histone H3 at lysine 27 (H3K27me3), a hallmark of repressed chromatin. Although overexpression of PRC2 components has been implicated in cancer initiation, progression and metastasis how PRC2 is recruited to its target genes remains poorly understood. Recent work suggests the interaction of PRC2 with the long-non coding (lnc) RNA HOX transcript antisense intergenic RNA (HOTAIR) could serve as a potential guide to silence target genes, but the mechanism has yet to be defined. In this study, we performed whole transcriptome RNA-seq analysis of isogenic cisplatin- sensitive and -resistant A2780 ovarian cancer (OC) cell lines. Of the lncRNA examined, HOTAIR was markedly induced in the cisplatin resistant cells (5-fold upregulation compared to the cisplatin-sensitve A2780 parental line). shRNA depletion of HOTAIR resensitized the drug-resistant cells to cisplatin. Based on analysis of the HOTAIR promoter using luciferase assays, we identified two putative NF-κB binding sites and three E-box binding sites. To further investigate whether these sites contributed to HOTAIR expression, platinum-sensitive cells were treated with different growth factors and cytokines (0 to 48hrs) and analyzed for changes in HOTAIR expression. Treatment with TNF-α, a well-known inflammatory cytokine and established activator of NF-κB, resulted in 16 fold-induction of HOTAIR. In accord with these results, we observed significant enrichment of both NF-κB and cMYC on the HOTAIR promoter using ChIP assay. Pharmacological inhibition of cMYC and NF-κB reduced HOTAIR expression by 2-fold, and siRNA depletion of the NF-κB p65 subunit or cMYC similarly reduced (P&amp;lt;0.01) HOTAIR expression. Based on these results, we hypothesize a positive feedback loop for HOTAIR transcriptional regulation: HOTAIR inhibits Iκ-Bα (an inhibitor of NF-κB) allowing the lncRNA to enter the nucleus and activate not only its own expression but also cMYC expression to further drive HOTAIR expression as well as cMYC-inducible genes. We are currently testing this hypothesis. We believe this novel pathway, whose components have been implicated in numerous other cancers, may be involved in cisplatin-resistant OC. Citation Format: Ali R. Ozes, Dave Miller, Cong Guo, Anurag Bhattrai, Yunlong Liu, Kenneth P. Nephew. The transcriptional regulation of the long non-coding RNA HOTAIR in ovarian cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5189. doi:10.1158/1538-7445.AM2013-5189
Prognosis of children with acute lymphoblastic leukemia (ALL) and intrachromosomal amplification of chromosome 21 (iAMP21)
Authors: Anthony V. Moorman, Susan M. Richards, Hazel M. Robinson, Jon C. Strefford, Brenda E. S. Gibson, Sally E. Kinsey, Tim O. B. Eden, Ajay J. Vora, Christopher D. Mitchell, Christine J. Harrison
Journal: Blood (2006)
Lung Cancer Etiology
Authors: Habibul Ahsan, Duncan C. Thomas
Journal: JAMA (2004)

Chi-Ling Chen, PhD; Lin-I Hsu, PhD; Hung-Yi Chiou, PhD; Yu-Mei Hsueh, PhD; Shu-Yuan Chen, PhD; Meei-Maan Wu, PhD; Chien-Jen Chen, ScD; for the Blackfoot Disease Study Group
Intramural Hemorrhage of the Small Intestine
Authors: Morgan Eiland
Journal: JAMA (1978)

IN 1954, Felson and Levin<sup>1</sup>described certain roentgenographic findings that were considered characteristic of intramural hemorrhage in the gastrointestinal tract. Since then others have described additional typical findings and have suggested pathophysiological processes to account for them. Although there is some disagreement as to how specific the diagnosis can be, there is agreement on the general appearance of intramural hemorrhage on plain films and barium contrast studies. With increasing awareness of this entity has come an increase in the number of pathological conditions shown to predispose to intramural hemorrhage. These conditions include trauma, anticoagulation therapy, hemophilia, Henoch-Schönlein purpura, idiopathic thrombocytopenic purpura, leukemia, lymphoma, cytotoxic therapy for neoplasm, polyarteritis nodosa, and myeloma. We will discuss the roentgenographic features of intramural hemorrhage, its course, and its mode of management, and will present illustrative cases. <h3>Report of Cases</h3><h3>Case 1.—</h3> An 80-year-old woman was admitted to the hospital with a three-day history
The mTOR inhibitor rapamycin significantly improves facial angiofibroma lesions in a patient with tuberous sclerosis
Authors: G.F.L. Hofbauer, A. Marcollo-Pini, A. Corsenca, A.D. Kistler, L.E. French, R.P. Wthrich, A.L. Serra
Journal: British Journal of Dermatology (2008)

Tuberous sclerosis complex (TSC) is an autosomal dominant disorder with an incidence of approximately one in 6000. It arises from a genetic abnormality involving either the TSC1 gene on chromosome 9 or the TSC2 gene on chromosome 16. The protein product of TSC1 is hamartin and that of TSC2 is tuberin. In cells, hamartin and tuberin form a complex which inhibits the mammalian target of rapamycin (mTOR), a central controller of cell growth and proliferation. Angiofibroma affects 70-80% of patients with TSC, typically on the face. We report a patient with TSC with recurrent life-threatening haemorrhage from both kidneys due to extensive angiomyolipoma formation leading to bilateral nephrectomy and renal transplantation. Immunosuppressive treatment with rapamycin, a specific mTOR inhibitor, initiated because of renal transplantation, reduced facial angiofibroma dramatically.
Chronic Hepatitis C Virus Infection
Authors: Robert M. Kleinhaus
Journal: Annals of Internal Medicine (2003)

Letters2 September 2003Chronic Hepatitis C Virus InfectionRobert M. Kleinhaus, MDRobert M. Kleinhaus, MDFrom Crouse Hospital; Syracuse, NY 13210.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-139-5_Part_1-200309020-00020-w1 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:I have noticed an interesting phenomenon that was not mentioned in Steven Herrine's excellent review of chronic hepatitis C virus (HCV) infection (1). I have several patients (and one husband and wife) who had positive results on HCV enzyme immunoassay and recombinant immunoblot assay. Although results of tests for both HCV RNA (reverse transcriptase polymerase chain reaction) and signal amplification (branched-chain DNA) were negative, HCV genotyping revealed a specific HCV genotype. (Initially, the genotyping was done by accident!) Some of the specialists to whom I referred the patients told them they do not have HCV infection, but ...Reference1. Herrine SK. Approach to the patient with chronic hepatitis C virus infection. Ann Intern Med. 2002;136:747-57. [PMID: 12020143] LinkGoogle Scholar Author, Article, and Disclosure InformationAffiliations: From Crouse Hospital; Syracuse, NY 13210. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoApproach to the Patient with Chronic Hepatitis C Virus Infection Steven K. Herrine Metrics 2 September 2003Volume 139, Issue 5_Part_1Page: W-67KeywordsChronic hepatitis CDNADNA amplificationEnzyme immunoassayGenotypingInfectious hepatitisRNAReverse transcriptase polymerase chain reaction ePublished: 2 September 2003 Issue Published: 2 September 2003 Copyright & PermissionsCopyright © 2003 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Platelet-derived microparticles induce angiogenesis and stimulate post-ischemic revascularization
Authors: A BRILL, O DASHEVSKY, J RIVO, Y GOZAL, D VARON
Journal: Cardiovascular Research (2005)

Platelet activation is accompanied by the release of microparticles. However, little is known about the role of platelet-derived microparticles (PMP) in the regulation of angiogenesis and related clinical situations. The aim of our study was to evaluate the effect of PMP on angiogenesis and to analyze its mechanisms.Both in vitro (rat aortic ring model, cell invasion test) and in vivo (agarose bead transplantation, artificial cardiac ischemia in Sabra rats) approaches were used in the study.A dose-dependent pro-angiogenic effect of PMP was observed in the rat aortic ring model. This effect could be eliminated by inhibition of VEGF, bFGF, and PDGF, but not heparanase. PMP exerted their effect via PI 3-kinase, Src kinase, and ERK, whereas protein kinase C and p38 were not involved. Moreover, PMP induced invasion of endothelial cells through a layer of matrigel. This effect was mediated by VEGF, heparanase, and PDGF, but not bFGF. Furthermore, PMP induced angiogenesis in an in vivo model in which agarose beads containing PMP were transplanted subcutaneously into mice. In addition, the effect of PMP on angiogenesis was evaluated in the model of in vivo chronic myocardial ischemia in rats. Ischemia induced a decrease in the number of functioning capillaries (34+/-21.5 vs. 157+/-42.0 per view field), but their amount increased after injection of PMP into the myocarium (97+/-27.3; p<0.001 vs. ischemia without PMP).PMP induce angiogenesis both in vitro and in vivo. Injection of PMP into the ischemic myocardium might improve the process of revascularization after chronic ischemia.
Kathleen Jessie Rigg
Authors: Michael Herbertson
Journal: BMJ (2005)

Concentration camp survivor and psychiatrist who became a high profile campaigner on child abuseThe Dutch psychiatrist and psychotherapy professor Dries van Dantzig once confessed to a colleague that he survived Neuengamme concentration camp "by making myself invisible"-which makes his survival seem all the more remarkable, as later he was rarely out of the public arena as a tireless and hard hitting promoter of mental health care.Imprisonment followed by years in a tuberculosis sanatorium may have sharpened the sense of injustice that reportedly drove Van Dantzig in his later public life.Today he is remembered as a merciless debater, publicist, political lobbyist, and film critic.His refusal to take "no for an answer" ensured that mental health and especially child abuse had a higher place on the political agenda of postwar Holland than it otherwise might have had.Van Dantzig was renowned for glaring from the front row at conference speakers with whom he disagreed.He made countless television appearances, and seemed to know a remarkable number of government ministers personally.The simple theme that underpinned his career was that, compared with somatic illness, psychiatric illness was largely ignored and underfunded.He illustrated this by arguing that every year more children would be killed by abuse than would ever die from Creutzfeldt-Jakob disease, which attracted millions more in funding.He suggested that up to 30% of the population had a neurosis, and that cases of child abuse could result in 50 to 80 deaths a year.Dries van Dantzig was born in 1920 into a progressive and left leaning family in Gouda.His education in Amsterdam was disrupted as the second world war broke out while he was still in his teens.He was eventually arrested for his involvement with the resistance and sent to Neuengamme near Hamburg.Of the 5500 Dutchmen sent there only 600 survived.Van Dantzig was among the few who did because, he later claimed, he was able to blend into the background amid such horrors as collecting dead bodies to take to the ovens or eating the food of those who had died.Yet all the time he was sharpening both his observational skills and his acute sense of injustice.
614 SPLENIC STIFFNESS ASSESSED BY ARFI (ACOUSTIC RADIATION FORCE IMAGING) CORRELATES WITH PORTAL PRESSURE IN CIRRHOSIS
Authors: A. Borghi, F. Piscaglia, L. Venerandi, C. Palama, V. Salvatore, M. Renzulli, C. Mosconi, R. Schiumerini, P. Zappoli, T. Carolina, L. Ricciardiello, A. Pecorelli, A. Collecchia, D. Festi, P. Andreone, L. Bolondi
Journal: Journal of Hepatology (2012)
Type-1 hepatorenal syndrome associated with infections in cirrhosis: Natural history, outcome of kidney function, and survival
Authors: Rogelio Barreto, Claudia Fagundes, Mónica Guevara, Elsa Solà, Gustavo Pereira, Ezequiel Rodríguez, Isabel Graupera, Marta Martín-Llahí, Xavier Ariza, Andrés Cárdenas, Javier Fernández, Juan Rodés, Vicente Arroyo, Pere Ginès
Journal: Hepatology (2013)

Type-1 hepatorenal syndrome (HRS) is a common complication of bacterial infections in cirrhosis, but its natural history remains undefined. To assess the outcome of kidney function and survival of patients with type-1 HRS associated with infections, 70 patients diagnosed during a 6-year period were evaluated prospectively. Main outcomes were no reversibility of type-1 HRS during treatment of the infection and 3-month survival. Forty-seven (67%) of the 70 patients had no reversibility of type-1 HRS during treatment of the infection. [Correction to previous sentence added March 10, 2014, after first online publication: "Twenty-three (33%)" was changed to "Forty-seven (67%)."] The main predictive factor of no reversibility of type-1 HRS was absence of infection resolution (no reversibility: 96% versus 48% in patients without and with resolution of the infection; P < 0.001). Independent predictive factors of no reversibility of type-1 HRS were age, high baseline serum bilirubin, nosocomial infection, and reduction in serum creatinine <0.3 mg/dL at day 3 of antibiotic treatment. No reversibility was also associated with severity of circulatory dysfunction, as indicated by more marked activity of the vasoconstrictor systems. In the whole series, 3-month probability of survival was only 21%. Factors associated with poor prognosis were baseline serum bilirubin, no reversibility of type-1 HRS, lack of resolution of the infection, and development of septic shock after diagnosis of type-1 HRS.Type-1 HRS associated with infections is not reversible in two-thirds of patients with treatment of infection only. No reversibility of type-1 HRS is associated with lack of resolution of the infection, age, high bilirubin, and no early improvement of kidney function and implies a poor prognosis. These results may help advance the management of patients with type-1 HRS associated with infections.
Parathyroid cysts--medical diagnosis and management
Authors: S. S. Stoffer
Journal: JAMA (1979)

To the Editor.—  We would like to augment the data in the provocative article by Ginsberg et al entitled Parathyroid Cysts: Medical Diagnosis and Management (240:1506, 1978). We have aspirated seven cystic nodules overlying the thyroid area and measured their parathyroid hormone content. In five cases, the cyst intact parathyroid hormone level 1 (x± SD, 281.0±107.2 pg Eq/mL) was approximately the same as the serum level (x±SD, 284.5±60.5 pg Eq/mL; normal range, 163 to 347 pg Eq/mL). One of these aspirates had a clear, watery color suggestive of a parathyroid cyst. In another of these cases later surgically shown to be benign microfollicular thyroid adenoma, both intact parathyroid hormone and carboxyl-terminal parathyroid hormone 2 were similar in serum and cyst fluid.  In two cases, the cystic fluid intact parathyroid hormone level was sharply greater than the serum level. In one case, the serum intact parathyroid hormone level was 192 pg Eq/mL and
Use of vaccinia virus vectors to study protein processing in human disease. Normal nerve growth factor processing and secretion in cultured fibroblasts from patients with familial dysautonomia.
Authors: R H Edwards, W J Rutter
Journal: Journal of Clinical Investigation (1988)

Familial dysautonomia is a hereditary disorder that affects autonomic and sensory neurons. Nerve growth factor (NGF) is required for the normal development of sympathetic and sensory neurons and it has been postulated that an abnormality involving NGF may be responsible for familial dysautonomia. Previous studies have shown that the beta-NGF gene is not linked to the disease. However, NGF appears to be abnormal by immunochemical assays; the putative altered form of NGF could result from a disturbance in the processing pathway. To study the processing of the 35-kD glycosylated NGF precursor and the secretion of NGF in familial dysautonomia, we have employed a recombinant vaccinia virus vector to express high levels of NGF mRNA in primary fibroblast cultures from patients with the disorder; the processing pathway was then studied directly. Cells from several unrelated patients all produce the same 35-kD NGF precursor, process this normally to NGF within the cell, and release NGF into the medium. There are no differences in the ability of cells from patients and from unaffected relatives to process and secrete NGF. The use of similar recombinant vaccinia virus vectors to express proteins at high level in primary cell lines should facilitate the detection of posttranslational processing defects in a variety of human disorders.
Medical Missionary Service
Authors: Kenneth M. Scott
Journal: JAMA (1967)

THIS INQUIRY WAS SUBMITTED TO KENNETH M. SCOTT, MD, DIRECTOR, CHRISTIAN MEDICAL COLLEGE AND HOSPITAL, PUNJAB, INDIA, AND ROBERT B. WHITE, MD, FORMERLY MISSIONARY PHYSICIAN, BELGIAN CONGO. Whether you plan to become a short-term or a career medical missionary, you should have acquired professional knowledge and skills at least as good as the best available in the country to which you will go. The day has long passed when<i>any</i>medicine could be said to be better than no medicine at all. Prior experience in general practice or, better, full specialty training is a requisite for most service overseas. If you live in America, this means certification by an American specialty board, to be completed before going overseas or at least during the first furlough. This is important because of the increasingly prominent place that teaching has in most overseas medical institutions today. Whether you serve in a developing country
Topical rubefacient ointment: studies on haemostasis.
Authors: C R Singer, H Hambley, I D Walker, J F Davidson
Journal: BMJ (1985)

Campylobacter colitis with intestinal aphthous ulceration mimicking obstructionWe report what we believe to be the first case of aphthous ulceration of the intestine in a child with colitis due to campylobacter.This child had the characteristic mucosal lesions of herpetic gingivostomatitis, X ray film showing numerous aphthous ulcers (arrowed) present in colon in campylobacter colitis.which also have not been recorded before with this particular bowel infection.The stools did not contain blood or mucus, and the initial tentative diagnosis was of bowel obstruction. Case reportA 7 year old boy presented with a five day history of intermittent perumbilical pain and was referred to us because of suspected obstruction.Two other children attending his school and using the same dining room had acute enteritis.Within this cluster one child was eventually found to have campylobacter in his stool.Our patient had severe vomiting and became more than 10%O dehydrated.A swinging fever with a peak at 40'C was observed.As the colitis resolved clinically so did the oral problem.
Imaging and Quantification of Myocardial Perfusion Using Real-Time Three-Dimensional Echocardiography
Authors: Eran Toledo, Roberto M. Lang, Keith A. Collins, Georgeanne Lammertin, Ursula Williams, Lynn Weinert, Gil Bolotin, Patrick D. Coon, Jai Raman, Lawrence D. Jacobs, Victor Mor-Avi
Journal: Journal of the American College of Cardiology (2005)
Rheumatoid arthritis: an antigenic chameleon
Authors: Eugen Feist, Guenter Steiner
Journal: Annals of the Rheumatic Diseases (2014)

Nowadays, rheumatologists and immunologists have to be absorptive like a sponge—from a breathtaking progress in the basic research field to emerging approaches in diagnosis and treatment of complex disorders like rheumatoid arthritis (RA), there is always exciting and sometimes unexpected news to digest.

For those who are particularly interested in the autoimmune response in RA, we have to realise that we are engaged with a true chameleon. Since the discovery more than 15 years ago of humoral autoimmune reactivities to epitopes containing the unusual amino acid citrulline,1 many new autoimmune targets have been discovered and, even more important, also different post-translational modifications to push their antigenic properties. Meanwhile the best understood process represents enzymatic deimination of peptide—or protein-bound arginine to citrulline. In genetically prone individuals, this activity creates highly specific recognition motives for auto-antibodies (anti-citrullinated protein/peptide antibodies, ACPAs), which serve as excellent diagnostic markers, especially in the early phases of disease. Since citrullination, as a physiological process, is also triggered by …
Detection of specific IgE antibodies towards cat flea (<i>Ctenocephalides felis felis</i>) in patients with suspected cat allergy
Authors: W. Rlfsen, H. Schröder, C. Tibell, M. Tibell
Journal: Allergy (1987)

Cat flea sensitivity is considered one of the most important skin diseases in cats and dogs. Cat fleas, however, are also a growing allergen problem for humans. Cat flea-specific IgE antibodies were studied in serum samples from 70 patients with suspected cat allergy, using RAST-based techniques and the nitrocellulose immunoblotting method. Results from RAST studies, using cat and cat flea as allergosorbents, showed that 46% of the patients were RAST positive against both cat and cat flea. 9% of the patients were RAST positive only against the cat flea. The nitrocellulose immunoblotting experiments were in agreement with the RAST results showing specific IgE to cat flea. The results indicate that some cat-allergic patients have specific IgE both towards cat and cat flea but also that some of the patients with suspected cat allergy might have specific IgE towards the cat flea and not the cat. RAST-inhibition and immunoblotting experiments also indicate that the allergen composition of cat flea extract differs from that of cat extract, even if common allergens have been detected, leading to cross-reactivity in some sera.