tbuckley/cabot-search huggingface dataset:
Abstract LB-293: Perfluorocarbon labeling of human α-type-1-polarized dendritic cells and conventional dendritic cells for in vivo trafficking by fluorine MRI
Authors: Brooke M. Helfer, Anthony Balducci, Pawel Kalinski, Amy Wesa
Journal: Cancer Research (2011)

Abstract Human dendritic cells (DC) have yet to live up to their clinical promise as an immunotherapeutic intervention against cancer, in spite of their in vitro profiles of their ability to effectively prime tumor-specific T cell responses. It is still unclear which is the best route of administration for the most efficient interaction of DC with tumor-specific T cells in patients, and whether the migratory capacity and localization of DC in patients correlates with clinical responsiveness. Here we utilize a perfluorocarbon (PFC) magnetic resonance imaging (MRI) tracer agent which may enable the assessment of the migration and localization of α-type-1-polarized dendritic cells (αDC1) in the clinic. αDC1 producing high levels of interleukin-12p70 with putative strong lymph node homing capabilities, are currently being evaluated clinically for anti-cancer activity. In vivo results demonstrate the capability of 19F MRI to detect and quantify labeled cells with moderate sensitivity, detecting fewer than 1×104 cells per voxel. The lack of endogenous fluorine in the body allows highly-specific 19F MR detection of label and when paired with proton MRI provides clear and quantitative cell localization within their anatomical context. The inertness of this tracking agent on the functional capacity of DC was examined in a panel of in vitro studies. The use of the MRI tracer with both conventional DC and αDC1 indicated similar labeling efficiencies with no observed effect on the DC's ability to stimulate T cell expansion, produce IL-12p70, process antigen or express co-stimulatory and polarizing factors. These data support the clinical translation of this tracer agent to safely track and quantify the administration and migration of human αDC1 by non-invasive 19F MRI in the treatment of cancer patients. An imminent clinical trial of PFC-labeled αDC1 will provide cell tracking data in patients, facilitating advances in greater efficacy in the next generation of DC-based cellular vaccines. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr LB-293. doi:10.1158/1538-7445.AM2011-LB-293
High prevalence of mitochondrial diabetes mellitus in Japanese patients with major risk factors
Authors: M. Fukui, K. Nakano, H. Obayashi, Y. Kitagawa, N. Nakamura, H. Mori, S. Kajiyama, S. Wada, M. Fujii, K. Yoshimori, T. Kanaitsuka, H. Shigeta, M. Kondo
Journal: Metabolism (1997)
Personality Types and Coronary Artery Disease
Authors: Ernest H. Friedman
Journal: JAMA (1972)

<h3>To the Editor.—</h3> In a recent article (<b>217</b>:929, 1971), Meyer Friedman and his colleagues reported a difference in growth hormone responses in 12 subjects with extreme type A behavior pattern compared with 12 subjects with type B extreme behavior pattern. They previously published similar findings with regard to serum cholesterol, triglycerides, phospholipids, and serum beta and other low-density lipoprotein lipids (<b>169</b>:1286-1296, 1959;<b>184</b>:934-938, 1963). Available data suggest that differences in serum cholesterol only can be demonstrated in the extreme behavior pattern subtypes and are not different in the milder expressions (<b>195</b>:86-92, 1966); the reader gains the impression that extreme type A compared with extreme type B subjects are clearly at higher coronary risk. A 4 1/2-year follow-up report of their prospective study demonstrates that the higher incidence of coronary artery disease occurs among subjects exhibiting the milder expressions of type A behavior pattern, but not in
Fourteenth Biennial Report of the Board of State Commissioners of Public Charities for the Years 1895 and 1896.
Authors: Arthur R. Reynolds
Journal: JAMA (1897)

For the last biennial period Illinois appropriated for State charities the sum of $2,962,550. This is a large amount, yet it is only one factor in the incalculable sum which the State by private and public contribution devotes to the care of the dependent classes. Scientific investigation has demonstrated its economic value in discovering sources of disease and disability and in suggesting methods of prevention. We submit that the State can not afford to conduct its charitable institutions merely for the immediate and direct alleviation of suffering and the protection of the inmates, primary and vital as this purpose is. The State has in these institutions great resources for research and study. Their proper use in furthering medical education, in promoting scientific investigation is due to the future of society and to the taxpayers of Illinois. Such use means no lack of consideration for the sick and helpless charges of
Abstract P4-09-06: No inverse association between breast cancer risk and n-3 unsaturated fatty acids in erythrocytes among Japanese women
Authors: C Koriyama, Y Kijima, S Natsugoe, S Akiba
Journal: Cancer Research (2013)

Abstract Dietary intake of fish, a major source of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), has been proposed to reduce the cancer risk. In contrast to the animal studies, their protective effects for breast cancer are equivocal in epidemiological studies. In a case-control study, we examined the association between the ratio of DHA (EPA) to aracidonic acid (AA) in erythrocytes and breast cancer risk in Kagoshima, Japan. All subjects, 147 breast cancer cases and 253 controls, were interviewed and information on lifestyles including smoking and alcohol drinking habits, menstrual and reproductive history, family history of cancer, and physical activity, was collected using a questionnaire. Erythrocyte fatty acids were measured by gas-liquid chromatography. According to the results of questionnaire survey, there was no inverse association between the frequency of fish intake and the risk of breast cancer. In pre-menopausal women, there was no significant association between DHA / AA and breast cancer risk (P for trend = 0.953). For post-menopausal women, although the first quartile of DHA / AA showed the lowest breast cancer risk, the risk was the highest in the second quartile and decreased after that. There was no association between EPA / AA and breast cancer risk in both pre- and post-menopausal women. These results suggested no inverse linear association between DHA(EPA) / AA in erythrocytes and breast cancer risk. Citation Information: Cancer Res 2013;73(24 Suppl): Abstract nr P4-09-06.
Harry Berrington (“Berry”) Stoner
Authors: Miles Irving
Journal: BMJ (2005)

Former director Medical Research Council trauma unit, Manchester (b 1919; q Sheffield 1942; MD, FRCPath, FRCS), d 9 July 2004.​2004.




Figure 1




Professor Harry Berrington Stoner was a world authority in trauma research. In 1953 he became head of the MRC experimental pathology of trauma unit in Carshalton, Surrey. The work on shock carried out at Carshalton was the focus of worldwide attention, but after 20 years was reaching its limits because it was confined to animal experimentation. The chance to transfer it to a clinical environment came with the creation at Manchester University of the MRC trauma unit. Major clinical outcomes of Stoner's work here included the development of an acclaimed sepsis scoring system. He leaves a daughter and two grandchildren.
Histopathological Changes in Sarcolemma and Sarcoplasm in Coxsackie Virus Infection
Authors: B. Strandberg
Journal: Annals of the Rheumatic Diseases (1954)

contributed towards the elucidation of its epidemiology and serology.Moreover, in acute anterior poliomyelitis the virus could be shown in Bornholm disease (myalgia epidemica;Sylvest, 1933) and in certain forms of gastro- enteritis.Two reports on the symptomatology of pure Coxsackie virus infection appeared in 1950.Shaw and others (1950) described a laboratory infection in six assistants engaged on the isolation of C.-virus.The symptoms were raised temperature, feeling of general weakness, lack of appetite, pains in the throat, single instances with stiffness of the neck, and in some cases diarrhoea, and 2 days later pains localized in the muscles of the abdomen, thorax, and extremities.Hueber and others (1951) reported the appearance of an epidemic C.-virus infection.The C.-virus has since been isolated during epidemics in Switzerland (Thelin and Wirth, 1951), and during a typical outbreak of Bornholm disease in England and Scotland (Brown, Liddle, and Tobin, 1952;Davies and Warin, 1951).Up till now, fourteen antigenically different strains of varying toxicity and tissue affinity have been isolated.One strain (Texas) has shown a decided affinity for the striated skeletal musculature on July 12,
394. Neuron-Specific, In Vivo Gene Delivery Mediated by Non-Viral Vectors
Authors: In-Kyu Park, Jurate Lasiene, Jennifer M. Knox, Shinn-Huey Chou, Philip J. Horner, Suzie H. Pun
Journal: Molecular Therapy (2006)

The development of nucleic acid delivery vehicles for the central nervous system (CNS) is an important technology goal in the advancement of molecular therapies for neurological diseases. An existing challenge to CNS delivery is efficient and targeted uptake of vehicles by neurons. In this work, two peptide-based targeting ligands were evaluated for their ability to mediate neuron-specific delivery. The neurotensin (NT) peptide undergoes rapid internalization via its high-affinity neurotensin receptor, which is predominantly expressed by neurons. A novel peptide sequence identified by phage display with potential neuron targeting abilities identified was also evaluated. Peptides were conjugated to cationic polymers and the modified polymers were complexed with plasmid DNA. The resulting nanoparticles were characterized by gel electrophoresis, dynamic light scattering and zeta potential measurements. In vitro gene delivery to differentiated, neuron-like PC-12 cells was demonstrated. Nanoparticles containing fluorescently-labeled (Cy3) plasmids were injected unilaterally into murine spinal cord. In this procedure, 8-12 week old C57Bl/6 female mice anesthesized with Avertin and the T10-T11 inter-laminar space exposed. Then, mice were fitted into a stereotactic frame and nanoparticles were injected at the junction of the intermediate gray matter and the lateral funiculus with a stereotactically guided Hamilton's syringe fitted with a 33 gauge Hamilton's needle. After injecting for 2 min, the needle was left in place for another 1 min and withdrawn slowly to prevent reflux. Optimized formulations resulted in migration of nanoparticles from the injection site in both caudal and rostral directions. The distribution of nanoparticles in the CNS was assessed by confocal microscopy with staining for microglia (Iba-1), mature and reactive astrocytes (GFAP), and neurons (NeuN) 3 days and 7 days post-injection. Specific co-localization of nanoparticles in neurons was observed with limited uptake in ramified microglia. Current work includes evaluation of transgene expression efficiency in neurons. Thus, these targeted nanoparticles can potentially be used in vivo to deliver nucleic acids to neurons with potential applications in treatment of neurodegenerative diseases.
Iron deficiency anaemia and febrile convulsions: case-control study in children under 2 years
Authors: A. Pisacane, R. Sansone, N. Impagliazzo, A. Coppola, P. Rolando, A. D'Apuzzo, C. Tregrossi
Journal: BMJ (1996)

Febrile convulsions are the most common type of seizure and occur in 2-4% of all children.1 A family history of convulsions; maternal smoking; and alcohol consumption during pregnancy have been associated with febrile seizures,2 3 but the risk factors remain largely unknown.

Iron is involved in the metabolism of several neurotransmitters, and monoamine and aldehyde oxidase are reduced in iron deficiency anaemia4, which is common during the second and the third year of life and has been associated with behavioural and development disturbances.5 Thus we investigated the association between iron deficiency anaemia and febrile seizures by a case-control study.

All 156 children …
BLOODLETTING IN CHILDREN
Authors: HEINRICH STERN
Journal: JAMA (1910)

<h3>HISTORY OF BLOODLETTING</h3> The history of bloodletting prior to the nineteenth century gives but scant mention of the employment of this therapeutic measure in children. The very fact, however, that ancient and medieval medicine would under ordinary circumstances not dispense with depletion justifies us in assuming that the children were subjected to the same procedure, although little special reference to this custom can be found. In Stöffler's "Calendarium romanum magnum" (1518) and in the "Medicina magica" (1568) it is stated that astrology, which influenced and pervaded the entire theory and practice of medicine of the times, accepted the theory that, according to situation and conjunction of the heavenly bodies, there existed favorable, dubious and unfavorable depletion days; the various periods of life had different days on which bloodletting was to be preferentially performed. The phase between half and full moon was deemed especially to favor depletion of young persons, but
For Three Quarters of Century, Safety Experts Work to Keep Trains, Vehicles From Colliding
Authors: Phil Gunby
Journal: JAMA (1992)

THIS WEEK, in New Orleans, La, Operation Lifesaver is holding its seventh biannual symposium. Its theme, as the nation tries to cope with its traffic violence, is "Blueprint for the Future." Part of that blueprint certainly will involve trying to reduce train-vehicle collisions at the nation's railroad grade crossings. There were 5350 such collisions in the past year—an average of nearly 15 a day—and they claimed more than 600 lives, nearly 4½ times the toll from commercial airliner crashes. <h3>Many Crossings, High Risk</h3> At that, the number of grade crossing collisions generally has been declining from an average of about 8000 a year at the beginning of the 1980s. But there are about 300 000 such crossings in the nation, 40% of which are on private property, where warning markings are not required. This form of traffic violence is so serious that the Transportation Research Board, part of the National
Abstract 4144: Identification of molecules involved in PpIX accumulation in brain tumor induced by oral administration of 5-ALA
Authors: Tomonari Suzuki, Satoru Wada, Hidetaka Eguchi, Kenji Wakiya, Junichi Adachi, Kazuhiko Mishima, Ryo Nishikawa, Masahiko Nishiyama
Journal: Cancer Research (2011)

Abstract [Background and Purpose] 5-aminolevulinic acid (5-ALA) is one of leading compounds widely used in photodynamic diagnosis and therapy of solid tumors: 5-ALA is metabolically converted to protoporphyrin IX (PpIX), a fluorescent molecule in response to excitation with a laser beam of 405 nm wavelength, that accumulates specifically in tumor lesions. This is found to be very effective for intra-operative detection of malignant brain tumors such as glioblastoma multiforme (GBM). On the other hand, we occasionally encountered cancerous lesions that are apparently malignant under a white light, while showing no fluorescence of the PpIX during cyto-reductive operation of the GBM. In order to overcome this problem, we hereby searched for factors associated with either presence or absence of the PpIX fluorescence by means of comprehensive gene expression analysis. [Materials and Methods] Thirty-one patients of brain tumor (21 GBM, 3 pilocytic astrocytoma and 7 oligodendroglial tumors), orally-administrated with 5-ALA followed by operation under a fluorescence-guide, were enrolled in this study. Tumor specimens with or without the 5-ALA induced-fluorescence were collected from these patients and snap-frozen in liquid nitrogen. Total RNAs were extracted from the frozen tissues and were subjected to a comprehensive gene expression analysis using Whole Human Genome 4×44K Oligo Microarray (Agilent). We then compared gene expression levels between specimens with or without the fluorescence among individuals and selected candidate genes of which gene expression levels significantly differ between these. For validation, quantitative gene expression analyses were conducted for the candidates with real-time RT-PCR using gene specific-primers and probes. Knockdown experiments using specific siRNAs were also performed in cell lines derived from GBM to test their biological significance. [Results and Discussion] A total of 14 genes were initially selected as candidates that were associated with PpIX accumulation in GBM in the microarray analysis. Among these, CDH13 (cadherin 13) gene was expressed significantly higher in the fluorescence-positive GBMs than in negative ones. Knockdown of CDH13 gene expression in GBM cells treated with 5-ALA, attenuated the fluorescence intensity, indicating its possible role in PpIX accumulation. Further experiments are underway to elucidate molecular mechanisms of CDH13 in 5-ALA-induced PpIX fluorescence in brain tumor. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4144. doi:10.1158/1538-7445.AM2011-4144
THE EFFECT OF INITIAL TUBERCULOUS INFECTION ON SUBSEQUENT TUBERCULOUS LESIONS
Authors: J. ARTHUR MYERS
Journal: JAMA (1934)

The effects of tubercle bacilli on experimental animals under various conditions have been reported on, but the lifetime of the animals used is so short compared with that of man and the resistance and susceptibility of the different species vary so much that it is impossible to determine in them all that is needed to be known about tuberculosis in the human body. It has been believed for some time that tuberculous lesions that appear in the bodies of children are of two types, namely, the first infection and the reinfection types, but very little work has been done to determine the effect of one on the other. In 1921 we undertook a longitudinal study of tuberculosis in the bodies of children. Since that time we have examined and studied 12,095 children and have observed each type of tuberculosis in its various stages of development, as well as the relationship
The Interval-Force Relationship of the Heart: Bowdich Revisited.
Authors: G. WRIGHT
Journal: Cardiovascular Research (1993)

The Interval-Force Relationship of the Heart: Bowdich Revisited. GORDON WRIGHT GORDON WRIGHT Keele, UK Eds M I M Noble and W A Seed. (Pp 368; £70.00.) Cambridge: Cambridge University Press, 1992. ISBN 0-521-40022-8. Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 27, Issue 3, March 1993, Page 539, https://doi.org/10.1093/cvr/27.3.539 Published: 01 March 1993
Potassium and Diuretics
Authors: A. Eisinger
Journal: BMJ (1966)

<h3>Objectives</h3> Exposure assessment for patients with occupational lung cancer is challenging because of long latency period. Mineralogical study on lung tissue provided information of exposure in previous working environment. We tried to apply LA-ICP-MS to assess the metal content in the lung tissue. <h3>Methods</h3> Two workers suspected to have occupational lung cancers participated, one working in a nickel refinery industry for 25 years and the other working in a rubber industry for 15 years. After surgical treatment by lobectomy, the lung tissue was fixed by formalin and wax. Pulmonary meterologic studies were done directly on the fixed pathologic slides by LA-ICP-MS. Urine concentration of relevant metals of co-workers was also evaluated. <h3>Results</h3> The ore used in the nickel refinery industry is nickel oxide, which is contaminated with 2% of cobalt. Urinary metal analysis of co-workers in the nickel refinery industry showed elevated nickel and cobalt. LA-ICP-MS detected spatially paralleled peaks of nickel and cobalt in lung tissue from the worker in the nickel refinery industry. The rubber industry used chromium trioxide as pigment. The urinary chromium level of co-workers in the rubber industry showed elevated chromium. However, LA-ICP-MS did not identify peak of chromium in lung tissue from the worker of the rubber industry. <h3>Conclusions</h3> LA-ICP-MS is a convenient and feasible way to assess lung metal content directly on pathologic slide without sample preparation, especially for insoluble dusts of metal compounds.
Detection of latent function in acutely ischemic myocardium in the dog: comparison of pharmacologic inotropic stimulation and postextrasystolic potentiation.
Authors: S H Dyke, C W Urschel, E H Sonnenblick, R Gorlin, P F Cohn
Journal: Circulation Research (1975)

In poorly perfused myocardium with resultant ischemic dysfunction, augmentation of contractility can, under certain conditions, be used to detect viable but ordinarily noncontracting muscle. Two methods of inotropic augmentation, pharmacologic inotropic stimulation and postextrasystolic potentiation (PESP), were studied in acutely ischemic canine myocardium with controlled coronary blood flow. A caliper length gauge to record segmental shortening and left ventricle pressure was used to construct pressure-length loops. Acute regional ischemia depressed segmental function: early segmental shortening decreased (-20 plus or minus 0.02% [SE]) and frequent dyskinesia occurred. Restoring coronary blood flow corrected segmental shortening to control levels. During acute regional ischemia, PESP consistently augmented segmental function (+49 plus or minus 0.03%) and abolished dyskinesia. Pharmacologic inotropic stimulation with isoproterenol or calcium administered into the coronary arteries did not produce a comparable improvement in segmental function (+9 plus or minus 0.05%). Although early shortening markedly increased with pharmacologic stimulation, there was no consistent change in total shortening, and the area of the pressure-length loop decreased. Due to late dyskinesia, there was a decrease in injection shortening. Systemically administered pharmacologic agents accentuated early dyskinesia but caused no consistent change in total shortening. Unlike PESP, pharmacologic agents either worsened segmental function or caused responses that were minimum and inconsistent; such responses clearly cannot be used to identify viable ischemic myocardium.
Semen Donors as the Source of Sexually Transmitted Diseases in Artificially Inseminated Women: The Saga Unfolds
Authors: Laurene Mascola
Journal: JAMA (1987)

Some things in life are not as straightforward as they used to be. With the latest medical technology, sexual intercourse is no longer a prerequisite for a woman to acquire a sexually transmitted disease. Artificial insemination must now be included as a possible source of these infections. In 1986 in the United States, an estimated 60 000 inseminations by donor semen were performed in women who conceived and were delivered of a child<sup>1</sup>through this technique. Additionally, an unknown number of inseminations were performed in women who either did not conceive or did not carry their pregnancy to completion. No national data are available to estimate precisely the number of inseminations performed each year, the number of semen donors used, the number of practitioners using the procedure, or the risk of sexually transmitted diseases in the recipients. The report of Berry et al,<sup>2</sup>in this issue ofThe