tbuckley/cabot-search huggingface dataset:
Reliability of Angiotensin-Converting Enzyme Gene Polymorphism in Testing
Authors: Bernhard R. Winkelmann
Journal: Annals of Internal Medicine (1997)

Letters15 May 1997Reliability of Angiotensin-Converting Enzyme Gene Polymorphism in TestingBernhard R. Winkelmann, MD, Markus Nauck, MD, and Winfried Marz, MDBernhard R. Winkelmann, MDHerzzentrum Ludwigshafen, 67063 Ludwigshafen, GermanyKlinikum der Albert Ludwigs-Universitat, 79106 Freiburg, GermanySearch for more papers by this author, Markus Nauck, MDHerzzentrum Ludwigshafen, 67063 Ludwigshafen, GermanyKlinikum der Albert Ludwigs-Universitat, 79106 Freiburg, GermanySearch for more papers by this author, and Winfried Marz, MDHerzzentrum Ludwigshafen, 67063 Ludwigshafen, GermanyKlinikum der Albert Ludwigs-Universitat, 79106 Freiburg, GermanySearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-126-10-199705150-00022 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail IN RESPONSE:Odawara and Yamashita question the reliability of the methods we used for genotyping the insertion/deletion polymorphism of the ACE gene [1, 2]. It has been emphasized that using primers that flank the polymorphic region may result in overestimation of the DD genotype because of preferential amplification of the D allele [2, 3]. We therefore reassessed all our samples using the method proposed by Shanmugan and colleagues [2], as described in the Methods section of our report. We also used the method of Lindpaintner and associates [3] to retype the samples that were previously found to be DD homozygous; ...References1. Rigat B, Hubert C, Corvol P, Soubrier F. PCR detection of the insertion/deletion polymorphism of the human angiotensin converting enzyme gene (DCP1) (dipeptidyl carboxypeptidase 1). Nucleic Acids Res. 1992; 20:1433. Google Scholar2. Shanmugan V, Sell KW, Saha BK. Mistyping ACE heterozygotes. PCR Methods Appl. 1993; 3:120-1. Google Scholar3. Lindpaintner K, Pfeffer MA, Kreutz R, Stampfer MJ, Grodstein F, LaMotte F, et al. A prospective evaluation of an angiotensin-converting-enzyme gene polymorphism and the risk of ischemic heart disease. N Engl J Med. 1995; 332:706-11. Google Scholar Author, Article, and Disclosure InformationAuthors: Bernhard R. Winkelmann, MD; Markus Nauck, MD; Winfried Marz, MDAffiliations: Herzzentrum Ludwigshafen, 67063 Ludwigshafen, GermanyKlinikum der Albert Ludwigs-Universitat, 79106 Freiburg, Germany PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 15 May 1997Volume 126, Issue 10Page: 828KeywordsAllelesAngiotensin converting enzymeGenotypingResearch design ePublished: 15 August 2000 Issue Published: 15 May 1997 Copyright & PermissionsCopyright © 1997 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Allergic reactions to cyclophosphamide: Delayed clinical expression associated with positive immediate skin tests to drug metabolites in five patients
Authors: N.Andrei Popescu, Michael G. Sheehan, Peter A. Kouides, John E. Loughner, John J. Condemi, Richard J. Looney, John P. Leddy
Journal: Journal of Allergy and Clinical Immunology (1996)

BACKGROUND: Cyclophosphamide (CP) is one of the relatively few drugs implicated in systemic allergic reactions for which the metabolites are well known. Formation of CP metabolites is a multistep, time-dependent process (hours) with significant interindividual differences. Although allergic reactions to CP have been recorded in 17 previous reports, skin testing with CP or its metabolites has been included in only five. We now describe five patients receiving monthly cycles of intravenous CP whose allergic reactions included clinical features of type I hypersensitivity but were atypical in their markedly delayed onset (i.e., 8 to 16 hours in patients 1 to 4 and 10 days in patient 5). OBJECTIVE: The objective was to investigate these late-developing clinical reactions by skin testing with CP and two of its major metabolites. METHODS: The five patients and a control group receiving intravenous CP uneventfully were studied by the same skin test protocol. RESULTS: The four individuals in the control group were unreactive to CP or its metabolites. All five patients with late-onset allergic reactions had positive immediate skin test results to CP metabolites but not to CP itself. We propose that the allergic reactions in patients 1 to 4 were mediated, wholly or in major part, by IgE antibodies reactive with allergens derived from time-dependent drug metabolites. The 10-day lag time in patient 5 is unexplained. Immunomodulation by the underlying malignancies or by the immunosuppressive drugs could have contributed. CONCLUSION: IgE-mediated allergic drug reactions may have a delayed onset if the allergen is a time-dependent drug metabolite, illustrated in this study by CP. (J ALLERGY CLIN IMMUNOL 1996;97:26-33.)
Combined dopamine and nitroprusside therapy in congestive heart failure. Greater augmentation of cardiac performance by addition of inotropic stimulation to afterload reduction.
Authors: R R Miller, N A Awan, J A Joye, K S Maxwell, A N DeMaria, E A Amsterdam, D T Mason
Journal: Circulation (1977)

The hemodynamic benefits of combining administration of dopamine with nitroprusside (NP) were evaluated in nine patients with chronic congestive heart failure due to ischemic, idiopathic myocardial or valvular cardiac disease. NP alone (68 microng/min) produced decline in left ventricular end-diastolic pressure (LVEDP) from 25.4 to 14.1 mm Hg (p less than 0.01) but modest increase in cardiac index (CI) from 2.41 to 3.02 L/min/m2 (P less than 0.05). Dopamine alone (6 microng/kg/min) caused an elevation of CI to 3.36 (P less than 0.01) but without decrease of LVEDP. Simultaneous infusion of the two agents resulted in favorable alterations in both hemodynamic variables: LVEDP decreased to 15.7 (P less than 0.01) and CI increased to 3.52 (P less than 0.01). It is concluded that dopamine substantially enhances the effectiveness of nitroprusside therapy in congestive heart failure by providing concomitantly the principal beneficial actions of the vasodilator and dopamine used separately. Thus combined dopamine with NP treatment considerably raises low CI while markedly reducing elevated LVEDP and provides a potentially efficacious pharmacologic modality for the treatment of severe congestive heart failure due to left ventricular dysfunction.
A NEW TONSIL SCISSORS, FORCEPS AND TONGUE DEPRESSOR.
Authors: C. C. STEPHENSON
Journal: JAMA (1903)

The operation for tonsillotomy with these instruments, and the advantages they possess, are briefly as follows: The forceps are made to seize the tonsil, when the handles are locked with fixation ratchet; the tonsil is then drawn out, the beak of scissors is passed behind, and the gland cut off entirely, as in cutting with an ordinary scissors. The beak prevents the tonsil from slipping from between the blades of scissors, and so much of the tonsil can be removed as is desired. While holding it with the forceps the tonsil is completely under control of the operator; thus there is no receding of the gland as in the ring of a tonsillotome. The simplicity and ease in operating commend the scissors and forceps, while with them the removal of entire gland without trouble is a desideratum. The handle of the tongue depressor is octagon, and curves outward, thus allowing an examination of the
Prospective isolation and molecular characterization of hematopoietic stem cells with durable self-renewal potential
Authors: David G. Kent, Michael R. Copley, Claudia Benz, Stefan Wöhrer, Brad J. Dykstra, Elaine Ma, John Cheyne, Yongjun Zhao, Michelle B. Bowie, Yun Zhao, Maura Gasparetto, Allen Delaney, Clayton Smith, Marco Marra, Connie J. Eaves
Journal: Blood (2009)
Answer is still out regarding BCG's possible anticancer role
Authors: Phil Gunby
Journal: JAMA (1982)

Bacillus of Calmette-Guérin (BCG) vaccine's possible role in the prophylaxis of some cancers is no less controversial than its longtime use in the attempt to prevent tuberculosis. The most recent forum to address the BCG-againstcancer question was the Seventh Chicago Cancer Symposium, sponsored by the Institute for Tuberculosis Research (ITR) of the University of Illinois at Chicago, the International BCG Committee, and the Illinois Cancer Council. The attenuated (BCG) strain of<i>Mycobacterium bovis</i>prepared by French scientists Albert Calmette and Camille Guérin first was administered to a human in Paris in May 1921. But controversy developed after that as a series of studies over the years produced varying results, indicating that BCG's protective efficacy against tuberculosis was not always great. Contamination of the vaccine with the virulent Kiel strain of<i>Mycobacterium tuberculosis</i>in a German laboratory caused 72 deaths in Lübeck in 1930, further adding to the debate (<i>Semin Oncol</i>
Re-epithelialisation of Barrett's oesophagus
Authors: C KELTY
Journal: Gut (2001)

Editor,—We were interested to read the case report by Van Laethem and colleagues of a carcinoma arising under a re-epithelialised segment of Barrett's oesophagus ((2000) Gut 46:574–577; [OpenUrl][1][CrossRef][2][PubMed][3][Web of Science][4]). This raises issues in … [1]: {openurl}?query=rft.jtitle%253DThe%2BAmerican%2Bjournal%2Bof%2Bgastroenterology%26rft.stitle%253DAm%2BJ%2BGastroenterol%26rft.aulast%253DByrne%26rft.auinit1%253DJ.%2BP.%26rft.volume%253D93%26rft.issue%253D10%26rft.spage%253D1810%26rft.epage%253D1815%26rft.atitle%253DRestoration%2Bof%2Bthe%2Bnormal%2Bsquamous%2Blining%2Bin%2BBarrett%2527s%2Besophagus%2Bby%2Bargon%2Bbeam%2Bplasma%2Bcoagulation.%26rft_id%253Dinfo%253Adoi%252F10.1111%252Fj.1572-0241.1998.525_b.x%26rft_id%253Dinfo%253Apmid%252F9772036%26rft.genre%253Darticle%26rft_val_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Ajournal%26ctx_ver%253DZ39.88-2004%26url_ver%253DZ39.88-2004%26url_ctx_fmt%253Dinfo%253Aofi%252Ffmt%253Akev%253Amtx%253Actx [2]: /lookup/external-ref?access_num=10.1111/j.1572-0241.1998.525_b.x&link_type=DOI [3]: /lookup/external-ref?access_num=9772036&link_type=MED&atom=%2Fgutjnl%2F48%2F4%2F580.2.atom [4]: /lookup/external-ref?access_num=000076315900005&link_type=ISI
Prevalence of hepatitis G virus (HGV) RNA and of anti-E2 antibody in various populations at low or high risk of transmitted viruses
Authors: F. Roudot-Thoraval, L. Morand-Joubert, Y. Brossard, M. Mariotti, M. Parnet, R. Girot, P. Loiseau, J.J. Lefrère
Journal: Journal of Hepatology (1998)
Bladder Tumor Infiltrating Mature Dendritic Cells and Macrophages as Predictors of Response to Bacillus Calmette-Guérin Immunotherapy
Authors: Cherifa Ayari, Hélène LaRue, Hélène Hovington, Marc Decobert, François Harel, Alain Bergeron, Bernard Têtu, Louis Lacombe, Yves Fradet
Journal: European Urology (2009)
Predictive impact of allele-matching and EBMT risk score for outcome after T-cell depleted unrelated donor transplantation in poor-risk acute leukemia and myelodysplasia
Authors: T Lodewyck, M Oudshoorn, B van der Holt, E Petersen, E Spierings, P A von dem Borne, A Schattenberg, W Allebes, M Groenendijk-Sijnke, L Duinhouwer, R Willemze, B Lowenberg, L F Verdonck, E Meijer, J J Cornelissen
Journal: Leukemia (2011)
Military Sexual Trauma
Authors: Mike Mitka
Journal: JAMA (2010)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Shaving can be safer head lice treatment than insecticides
Authors: Anthony Lwegaba
Journal: BMJ (2005)

Health inequalities under New Labour Relative rather than absolute gaps are important over time and placeEditor-We agree with Shaw et al that the method of measuring inequality in health outcomes used in setting the government targets is problematic (A Low, UKPHA (UK Public Health Association), 13th annual public health forum, Gateshead, April 2005). 1 However, the government's targets are based on reducing relative gaps in life expectancy and infant mortality. 2 The slope index of inequality (SII) used by Shaw et al measures absolute gaps.
Alterations of Lactate Dehydrogenase Isoenzymes During Therapy Directed at Leukemia.
Authors: Herbert H. Spencer
Journal: Annals of Internal Medicine (1967)

s1 May 1967Alterations of Lactate Dehydrogenase Isoenzymes During Therapy Directed at Leukemia.Herbert H. Spencer, M.D. (Associate), C. J. D. Zarafonetis, M.D., F.A.C.P., W. H. Starkweather, M.S.Herbert H. Spencer, M.D. (Associate)Search for more papers by this author, C. J. D. Zarafonetis, M.D., F.A.C.P.Search for more papers by this author, W. H. Starkweather, M.S.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-66-5-1044_3 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptNormal human lymphocytes and granulocytes have specific lactate dehydrogenase (LDH) isoenzyme patterns. When leukemic cells were studied, changes in the isoenzymes were observed that appeared to be of prognostic value during chemotherapy. In 15 cases, during the transition of chronic granulocytic leukemia to the acute phase, a significant drop in LDH-5 occurred, with a concomitant increase in the relative amounts of LDHl, -2, and -3. The pattern of change in the isoenzymes depended upon the degree of cellular differentiation present while the total cellular LDH activity decreased. In the terminal blast phase of the disease, an intermediate isoenzyme pattern with... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: Herbert H. Spencer, M.D. (Associate); C. J. D. Zarafonetis, M.D., F.A.C.P.; W. H. Starkweather, M.S.Affiliations: Ann Arbor, Michigan PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 May 1967Volume 66, Issue 5Page: 1044-1045KeywordsCellsChemotherapyGranulocytesLactate dehydrogenaseLymphocytes ePublished: 1 December 2008 Issue Published: 1 May 1967 PDF downloadLoading ...
Outbreaks of E coli
Authors: R L Salmon
Journal: BMJ (1997)

Verotoxin producing Escherichia coli causes severe gastrointestinal disease, often with frank blood in the stool (haemorrhagic colitis). In about 2-7% of cases infection is complicated by haemolytic uraemic syndrome,1 which has a mortality of 3-5%. The current outbreak of infection with E coli 0157 in Scotland,2 described as “the second largest recorded outbreak anywhere,” is the subject of an interim report by Professor Hugh Pennington's expert group, produced with commendable speed.

Perhaps inevitably, the group has been able to discharge some parts of its remit better than others. With various legal proceedings pending, the first part of its remit—to examine the specific “circumstances which led to the outbreak”—receives only a brief reference. There are no descriptive or other data. Regarding the second part of its remit—“the implications for food safety and …
Expression of Snail in Upper Urinary Tract Urothelial Carcinoma: Prognostic Significance and Implications for Tumor Invasion
Authors: Takeo Kosaka, Eiji Kikuchi, Shuji Mikami, Akira Miyajima, Suguru Shirotake, Masaru Ishida, Yasunori Okada, Mototsugu Oya
Journal: Clinical Cancer Research (2010)

Abstract Purpose: There are few molecular markers known to predict upper urinary tract urothelial carcinomas (UTUC) prognosis. Snail, which contributes to epithelial–mesenchymal transition (EMT), has been documented in cancer progression, but not clear yet in UTUC. We therefore addressed the expression and biological significance of Snail in UTUC. Experimental Design: To elucidate the biological significance of Snail in UTUC, we examined the immunohistochemical expression of snail in UTUC and analyzed its clinical significance in 150 patients with UTUC. Biological effects of Snail in EMT and invasion were evaluated by using small interfering RNA (siRNA) specific for Snail in urothelial carcinoma cell lines and the Matrigel invasion assay. Results: Nuclear Snail staining was very weak in superficial UTUC. In contrast, strong Snail staining was observed in many of the nucleus of invasive UTUC. Snail expression was significantly higher in the high tumor stage, high grade, and in tumors showing lymphovascular invasion (LVI). Multivariate Cox regression analysis revealed that elevated Snail expression was a significant and an independent prognostic predictor of recurrence-free survival and cancer-specific survival. Patients with positive LVI and high Snail expression showed the worse outcome. Targeting of Snail mRNA expression in UMUC-3 cells with Snail-specific siRNA downregulated the mRNA expression of Snail, Vimentin, MMP2, and MMP9. Furthermore, the cells with siRNA for Snail showed decreased invasion activity in comparison with the cells transfected with a nontargeting siRNA. Conclusion: Snail-induced EMT represents a clinically relevant mechanism of UTUC progression and an attractive target for the treatment of patients with UTUC. Clin Cancer Res; 16(23); 5814–23. ©2010 AACR.
Type 2 iodothyronine deiodinase is highly expressed in human thyroid.
Authors: D Salvatore, H Tu, J W Harney, P R Larsen
Journal: Journal of Clinical Investigation (1996)

Type 2 iodothyronine deiodinase (D2) is a recently cloned selenodeiodinase thought to provide intracellular 3,5,3' triiodothyronine (T3) to a restricted group of tissues. We report here the presence of D2 mRNA in human thyroid at levels 50-150-fold higher than in placenta. Surprisingly, while type 1 deiodinase (D1) is known to be present in human thyroid, D2 has not been evaluated previously. D2 mRNA was especially high in thyroids from Graves' patients and in follicular adenomas. Stimulated thyroids had higher D2 to D1 mRNA ratios than normal or multinodular glands suggesting differential regulation of D1 and D2 expression. Microsomes from normal, Graves', and TSH-stimulated thyroids contained low Km D2 activity resistant to propylthiouracil (1 mM) or to inactivation by N-bromoacetyl T3, agents which block or inactivate D1. At 2 nM thyroxine (T4), 100 times the physiological-free T4 levels, 60-80% of T4 to T3 conversion in stimulated, but only 27% of that in normal thyroids, is catalyzed by D2. We conclude that intrathyroidal T4 to T3 conversion by D2 may contribute significantly to the relative increase in thyroidal T3 production in patients with Graves' disease, toxic adenomas, and, perhaps, iodine deficiency.
Patient passports would alert doctors to previous bone marrow transplantation
Authors: A. Smith, H. Somerville
Journal: BMJ (2001)

EDITOR—Hammadieh et al intended their letter to alert doctors to the fact that patients who have had successful bone marrow transplantation have peripheral blood cells originating from the donated bone marrow and that results of cytogenetic studies of these blood cells will relate to the donor.1

Unfortunately, only one of the two cases in the letter (case 1) was a cytogenetic case, involving a bone marrow transplant from a donor of the opposite sex. …
HOME CARE OF RHEUMATIC FEVER PATIENTS
Authors: Dennison Young
Journal: JAMA (1953)

In October, 1948, a two-and-a-half-year pilot project for home care of children with active rheumatic fever was instituted at Montefiore Hospital, New York City. This project was sponsored by the New York Heart Association and was an extension of the home care program begun at Montefiore Hospital in 1947.1The purpose of the project was to determine whether a standard of care comparable to that given in a general hospital or in a specialized institution for the care of patients with rheumatic fever could be given in the home with perhaps greater over-all benefit to the child. The program also covered the emotional and social problems of the rheumatic fever patient and his family. This report, however, is limited to the medical aspects of the program.  ORGANIZATION  The children included in the program were drawn from the geographical area of upper Manhattan and Bronx County of New York City,
Remarks on the Use of Carbolised Catgut in the Ligature of Arteries in their Continuity
Authors: J. R. Lane
Journal: BMJ (1877)

fie ¶ting that due supply of venous blood to a lung would seem to be quite as essential a condition for the physiological action of a lung as due supply of air can be, and that the dyspncea which people generally connect with insufficient air-supply may equally come from interference with blood-supply.Your contraction of capillaries, if of a spasmodic * Read at the Conjoint Meeting of the East and West Surrey Districts of the Soth-Eastern Branch.0