tbuckley/cabot-search huggingface dataset:
Abstract 2853: Monoclonal antibodies derived in neuN mice following successful T cell therapy of spontaneous breast tumors are specific for tumor progenitor cells.
Authors: Li-Xin Wang, Mona Patel, Michael Berk, Gregory E. Plautz
Journal: Cancer Research (2013)

Abstract There is considerable interest in developing approaches to specifically target tumor progenitor cells. Our previous results demonstrated that adoptive transfer of CD4+ and CD8+ T cells primed with a breast cancer progenitor vaccine inhibited the progression of spontaneous primary tumors in neuN mice. Interestingly, post-treatment serum but not pre-treatment serum was reactive with surface molecules on the vaccine cells. This indicates that the transferred T cells resulted in uptake of tumor antigens and stimulation of host B cells. To characterize the antigenic targets, we generated hybridomas from spleen cells derived from treated mice. Three candidate clones which were strongly reactive with a neu-negative variant tumor line were subjected to two rounds of limiting dilution cloning. The mAb prepared from hybridoma #1 (IgG1) and hybridoma #2 (IgG2b) were each reactive against two independent tumor lines derived from spontaneous breast tumors in neuN mice (C2D1 and MMC5) when these cells were maintained in serum-free culture medium as tumorspheres. There was no reactivity against the same tumor cell lines when cultured as adherent monolayers in serum-containing medium nor was there reactivity against 3T3 adherent or tumorsphere cultures. Our previous analysis of tumorsphere cultures of C2D1 and MMC5 indicated that they express a panel of markers associated with tumor progenitor cells and have markedly enhanced per-cell tumorigenicity. Two of these antibodies have been purified by protein G or Protein A columns and retain binding to C2D1-tumorsphere cells, but not to C2D1 adherent cells. Importantly, these two antibodies can also bind to cultured 4T1-tumorsphere cells, an additional breast cancer cell line arising initially in BALB/c mice. These studies will allow us to identity corresponding antigens and determine expression on a broader array of spontaneous breast carcinomas in neuN mice. The epitopes recognized by these mAbs, which are expressed on progenitor cells from multiple tumor lines, might have importance as an immunotherapy target. Citation Format: Li-Xin Wang, Mona Patel, Michael Berk, Gregory E. Plautz. Monoclonal antibodies derived in neuN mice following successful T cell therapy of spontaneous breast tumors are specific for tumor progenitor cells. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2853. doi:10.1158/1538-7445.AM2013-2853
C–11 Methionine Uptake in Granulomatous Disease
Authors: Sharon F. Hain, Andrew D. Beggs
Journal: Clinical Nuclear Medicine (2004)

Although uptake of F-18 FDG is well documented in granulomatous disease, there is little literature available regarding C-11 methionine positron emission tomography (PET) in these conditions. We present a case in which C-11 methionine imaging was performed for localization of a probable parathyroid adenoma, but other areas of uptake identified were subsequently found to be the result of sarcoidosis. Clinicians must therefore take care when interpreting C-11 methionine PET scans because granulomatous disease could cause false-positives.
"Anemia" of Renal Failure: A Physiological Adaptation.
Authors: B. von Hartitzsch
Journal: Annals of Internal Medicine (1973)

Meeting Abstracts1 May 1973"Anemia" of Renal Failure: A Physiological Adaptation.B. von Hartitzsch, M.D., J. W. Eaton, Ph.D., C. M. Kjellstrand, M.D., T. J. Buselmeier, M.D.B. von Hartitzsch, M.D.Search for more papers by this author, J. W. Eaton, Ph.D.Search for more papers by this author, C. M. Kjellstrand, M.D.Search for more papers by this author, T. J. Buselmeier, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-78-5-822_4 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptRecent information indicates that blood transfusions, which may induce hepatitis, bone marrow suppression, and isoimmune stimulation are unwarranted in the treatment of anemia of renal failure. Elevated serum phosphate levels, as well as hypoxia, stimulate enhanced red cell 2, 3-diphosphoglycerate (DPG) synthesis, with DPG concentration higher than those reported for similar anemias of other causes. Despite their low hemoglobin concentrations, the blood of these patients gives up almost as much oxygen per volume of whole blood as the blood of normal persons. The anemia of renal failure may be owing to the lack of hypoxic stimulus due to enhanced oxygen... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Minneapolis, Minnesota PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 May 1973Volume 78, Issue 5Page: 822-823KeywordsAnemiaAttentionBlood volumeBone marrowHemoglobinHepatitisHypoxiaOxygenPhosphatesRenal failure ePublished: 1 December 2008 Issue Published: 1 May 1973 PDF downloadLoading ...
GP is struck off after exposure by television documentary
Authors: C. Dyer
Journal: BMJ (2013)

A GP who was secretly filmed telling an undercover actor who presented with signs and symptoms typical of bowel cancer to “eat some mangoes” has been struck off by a panel of the Medical Practitioners Tribunal Service.

Inayat Inayatullah, 73, was investigated by Channel 4’s Dispatches programme as part of a documentary examining doctors who had returned to work after being previously disciplined by the General Medical Council. The programme aired in October 2011.

A fitness to practise hearing in 2006 …
Donough O'Brien
Authors: David C Morley
Journal: BMJ (2004)

Former professor of paediatrics University of Colorado, United States (b 1923; q Cambridge/St Thomas's Hospital, London, 1947), died from muscular wasting on 6 March 2004.​2004.




Figure 1




After qualifying and military service Donough specialised in paediatrics in London, where he was involved in the introduction of exchange transfusions for the newborn. Through a Harvard fellowship in 1953 he saw the potential for working in the United States. He moved to Denver in 1957 as assistant professor, before becoming professor in 1964. He retired as emeritus in 1991. In Colorado he founded and headed the Kennedy Stolinsky Center for Metabolic Diseases and the Barbara Davis Center for Childhood Diabetes. One of his many contributions to paediatrics was developing micromethods that made unpleasant venepuncture in small children rarely necessary. He leaves a wife, Madeline; two sons; and two grandchildren.
P5-13-02: Prediction of Dasatinib Sensitivity of Breast Cancer Based on a Novel Tyrosine Kinase-Activity Profiling Assay.
Authors: M Kawai, Y Torikoshi, M Notoya, K Gohda, NT Ueno, H Ishihara
Journal: Cancer Research (2011)

Abstract Background: Receptor tyrosine kinases and other membrane-associated tyrosine kinases are frequently overexpressed, activated, or mutated in cancer cells and cause aberrant signal transduction, which leads to cellular proliferation, angiogenesis, metastasis, and antiapoptosis. Blockage of these signals is considered an efficient strategy in cancer therapy, and to date, several tyrosine kinase inhibitors (TKIs) and antibody drugs targeting such kinases have been developed and are routinely used in clinics. However, the efficacy rates of the drugs are, unfortunately, limited. To provide optimum care for individual patients, an accurate prediction method for drug efficacy is now strongly demanded. Recent studies have shown that alteration of multiple kinases is involved in both primary and acquired drug resistance. Advantages of multi-targeted TKIs have also been reported. These findings indicate the need for a comprehensive evaluation of multiple kinases for predicting drug response. To achieve this, we developed a novel method of comprehensively profiling kinase activity. Materials and methods: We characterized the membranous tyrosine kinases of breast cancer cell lines using a newly established profiling assay. Briefly, crude membrane fractions were prepared and directly subjected to the assay with nonspecific substrate (Poly(Glu4-Tyr)). The profile of the kinases in the crude membrane fraction was obtained by inhibiting the total activity with 13 selected kinds of adenosine triphosphate antagonists, independently. The residual activity (RA) of membranous kinases was defined as the percentage of activity with/without TKI. Results: Nineteen breast cancer cell lines were classified as “sensitive” (n = 6) and “resistant” (n = 13) to dasatinib according to the definition in the publication (Cancer Res., 67 2226–38. 2007). The RA of tyrosine kinases targeted by different types of TKIs was determined by our assay, and we found that two RAs (src inhibitor 1 and PP1) showed statistically significant differences between the “sensitive” and “resistant” groups (p=0.017, and p=0.002, respectively, Student's t-test). The RA of the two TKIs were also significant predictors for dasatinib sensitivity in a receiver operating characteristic curve analysis (area under the curve: 0.846 for src inhibitor 1 and 0.910 for PP1). Since the major target of dasatinib are src family kinases, protein expressions of the src family of the cell lines were quantified by a Western blotting and compared between the groups. We found that the protein expression is not a statistically significant predictor of dasatinib sensitivity. Conclusion: We have shown that a comprehensive tyrosine kinase activity profiling assay of cancer cells can predict dasatinib sensitivity. We plan to validate this assay prospectively in patients with breast cancer who receive dasatinib. Citation Information: Cancer Res 2011;71(24 Suppl):Abstract nr P5-13-02.
Involvement of interleukin-21 in the epidermal hyperplasia of psoriasis
Authors: Roberta Caruso, Elisabetta Botti, Massimiliano Sarra, Maria Esposito, Carmine Stolfi, Laura Diluvio, Maria Laura Giustizieri, Valentina Pacciani, Annamaria Mazzotta, Elena Campione, Thomas T MacDonald, Sergio Chimenti, Francesco Pallone, Antonio Costanzo, Giovanni Monteleone
Journal: Nature Medicine (2009)
Abstract 2326: The first crystal structure of a telomeric quadruplex DNA with a metal-containing ligand
Authors: Nancy H. Campbell, Nurul H. Abd Karim, Gary N. Parkinson, Ramon Vilar, Stephen Neidle
Journal: Cancer Research (2011)

Abstract The inhibition of telomerase function in cancer cells via induction of a higher-order four-stranded quadruplex arrangement in the telomeric DNA substrate, requires the participation of a quadruplex-stabilizing ligand. We showed several years ago that certain metal complexes could fulfil this role and not only have selectivity for a quadruplex arrangement but had significant biological activity. We now report on the crystal structures of square-planar nickel (II) and copper (II) complexes containing salphen ligands with a human telomeric quadruplex. The structures show that the metal complexes are bound on an external G-quartet face of the quadruplex, and that the metal itself is positioned above the potassium-containing ion channel of the quadruplex, providing important stabilization. The planar groups of the ligand are stacked onto guanine bases, and the charged amino side-chains are positioned in the grooves of the quadruplex. These features are enabling the available structure-activity data to be rationalized and will be discussed in terms of the design of future complexes with enhanced activity. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2326. doi:10.1158/1538-7445.AM2011-2326
BONE-MARROW TRANSPLANTATION FOR INBORN ERROR OF PHAGOCYTIC CELLS ASSOCIATED WITH DEFECTIVE ADHERENCE, CHEMOTAXIS, AND OXIDATIVE RESPONSE DURING OPSONISED PARTICLE PHAGOCYTOSIS
Authors: A. Fischer, B. Descamps-Latscha, I. Gerota, C. Scheinmetzler, J.L. Virelizier, PhamHuu Trung, B. Lisowska-Grospierre, N. Perez, A. Durandy, C. Griscelli
Journal: The Lancet (1983)
Cecal Lithiasis Secondary to Cecal Stenosis
Authors: Adolf L. Gundersen
Journal: JAMA (1968)

A review of the causes and locations of intestinal stenosis revealed that it rarely is found in the colon and that none has been reported in the cecum below the ileocecal valve. Cecal lithiasis, below a cecal web, occurred in a 54-year-old man. Treatment consisted of linear colotomy and incision of the web, removal of stones, and transverse closure. Formation of stones and the late appearance of symptoms from the poorly drained cecal reservoir were due to the location of the stenosis.
Abstract 5510: Assessing the level of breast cancer awareness among recently diagnosed patients in Ain Shams University Hospital
Authors: Mohamed El-Shinawi, AlMoataz Youssef, Mohamed K. Aly, Mohamed Mostafa, Mohamed Alsara, Ahmed Yehia, Marc Hurlbert, Mona M. Mohamed, Reda Abd El-Tawab
Journal: Cancer Research (2012)

Abstract Introduction: 97% of Egyptian breast cancer patients are diagnosed at advanced stage, 70.6% possess positive lymph node metastasis on diagnosis while only 29.4% are free of metastasis reflecting late presentation of Egyptian patients. PURPOSE: Assess the level of knowledge about breast cancer among recently diagnosed breast cancer patients at Ain Shams University Hospitals. METHODS: In a cross-sectional, analytical observational study, we randomly enrolled 289 breast cancer patients with 45 of them who were recently diagnosed at Ain Shams University Hospitals meeting our inclusion criteria.Recurring patients or those unaware of diagnosis were excluded. Patients were interviewed using a specially designed questionnaire -of 75 questions over 60 minutes. Questionnaire gathered socio-demographic information, maternity history and tested knowledge of risk factors, signs and symptoms, screening options, management and prognosis of breast cancer was tested. Data was correlated to TNM staging. RESULTS: Patients’ age ranged between 30-72 years (mean 48±10.19). 40% were illiterate and only 15.6% finished higher education with 6-month average delay in seeking medical attention. Among the 45, TNM staging was assessed in 19 patients. Although 93% knew painless mass was symptom of breast cancer, 69% presented with painless mass and 47% knew someone with breast cancer prior to diagnosis, yet 24% thought they might have breast cancer. Regarding regular clinical breast exam 58% agreed to have it but 60% didn't know what was mammography, 56% didn't hear about breast self-exam and only 22% knew breast cancer had risk factors. Moreover, 58% thought treatment caused patients to be very ill, 29% thought breast cancer had good survival, yet 75% thought breast cancer is curable. Total breast cancer knowledge scores were low, with a median of 13 (out of 35) and mean of 12.64 ±5.12. CONCLUSIONS: Patients had acceptable awareness about breast cancer symptoms yet displayed gross ignorance about risk factors and poor awareness about screening, management and prognosis, correlating with their advanced TNM score. Therefore, raising breast cancer awareness and promoting education and early detection among our community is important. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5510. doi:1538-7445.AM2012-5510
Difference in the structural features of streptococcal M proteins from nephritogenic and rheumatogenic serotypes.
Authors: K M Khandke, T Fairwell, B N Manjula
Journal: The Journal of Experimental Medicine (1987)

The association of only certain M protein serotypes of group A streptococci with acute glomerulonephritis is very well recognized. Structural information on the M protein, a dimeric alpha-helical coiled-coil molecule, has come so far from three rheumatogenic serotypes, 5, 6, and 24. However, M proteins from the nephritogenic serotypes have not been well characterized. In the present study, we have isolated a biologically active 20,000 Mr pepsin fragment of type 49 M protein (PepM49), a nephritogenic serotype, and purified it to homogeneity using DEAE-Sephadex and gel filtration. The amino acid composition of PepM49 is similar to those of the rheumatogenic M protein serotypes PepM5, PepM6, and PepM24. However, the sequence of the NH2-terminal 60 residues of PepM49 shows little homology to any of these M protein serotypes, although the latter have significant homology among themselves. Nevertheless, PepM49 exhibits a strong heptad periodicity in its nonpolar residues, suggesting its overall conformational similarity with the other M molecules. During the course of the present studies, Moravek et al. (17) reported the NH2-terminal sequence of another M protein serotype, PepM1, which also does not exhibit much homology with the PepM5, PepM6, and PepM24 proteins. Our analysis of this sequence revealed that the PepM1 protein also exhibits a heptad periodicity of the nonpolar amino acids. A closer examination has revealed that the pattern of heptad periodicity in PepM49 and PepM1 proteins is more regular and more similar to each other than has been previously seen for the PepM5, PepM6, and PepM24 proteins. PepM1 is also a nephritogenic serotype. Taken together, these findings indicate an underlying conservation of the tertiary structure of the various M protein serotypes, despite the complexity in their antigenic variation and suggest that the nephritogenic M protein serotypes M1 and M49 may be further apart evolutionarily from the rheumatogenic serotypes 5, 6, and 24. The distinct differences in the structural features of the PepM1 and PepM49 proteins relative to the PepM5, PepM6, and PepM24 proteins are also suggestive of a correlation with the earlier broader classification of the group A streptococci into rheumatogenic and nephritogenic serotypes.
Gallium Imaging in a Patient With Tumor Lysis Syndrome
Authors: Pinar Ozgen Kiratli
Journal: Clinical Nuclear Medicine (2007)

Tumor lysis syndrome is a potentially life threatening disorder, characterized by hyperkalemia, hypermagnesaemia, hyperphosphatemia, elevated levels of serum creatinine and urea, indicating renal dysfunction because of rapid lysis of a large number of tumor cells. It is most likely to occur after chemotherapy of cancers highly responsive to chemotherapeutic drugs, such as malignant lymphomas. These patients should receive prophylactic treatment especially during their first course of chemotherapy, and laboratory tests should be performed daily to avoid this serious complication. Here, a patient with malignant lymphoma referred for gallium scan is presented. The imaging findings with association to the laboratory results and clinical progress suggested the diagnosis of tumor lysis syndrome.
The Cancer Syndrome
Authors: Philip Rubin
Journal: JAMA (1974)

THE TERM syndrome is applied to a constellation of features that result in a recognizable pathologic and clinical symptom complex. It is an association of characteristics that often alert the physician that certain disparate physical findings or complaints may not be chance events. Furthermore, seemingly remote pathophysiologic occurrences may offer a clue about the pathogenesis of the neoplastic state. The increasing awareness of syndromes in cancer patients has allowed for new diagnostic and investigative pathways. The debilitation of a cancer patient may not be related to the invasion of normal and vital organs by the neoplasm, but to the tumor's capability to create or be causally related to certain distant functional aberrations. The patterns of production of such features are distinctive and should be known in their relationship to alimentary-tract growth. Most syndromes described in medicine are related to genetic factors, and are recognizable mainly through identifiable hereditary transmission patterns.
The Primary Care Physician and Health Care Reform
Authors: Robert H. Brook
Journal: JAMA (2010)

WHATEVER FORM IT TAKES, HEALTH CARE REform will increase the number of Americans covered by health insurance. But there is concern that the legislation will not bend the cost curve—that is, will not reduce the growth of health care costs so that it more closely resembles the growth of the US gross domestic product (GDP). Currently, health care consumes about 16% of the GDP; advocates of bending the cost curve hope that in 2020 it will still consume roughly the same proportion. Increased health care coverage raises issues in addition to cost containment. Increased coverage will mean increased demand for primary care physicians. Virtually everyone would like to have a primary care physician—a trusted physician who provides comprehensive, continuous care. Many studies, including many with international comparisons, have established the benefits of primary care. However, it is increasingly difficult to convince graduates of US medical schools to choose primary care as their professional career path. Why is there such a disconnect between the demand for and supply of primary care physicians? Two reasons appear to predominate: scope of practice and salary.
SUBMUCOUS LIPOMA OF THE CECUM
Authors: H. R. DeLuca
Journal: JAMA (1933)

This case is presented because of its infrequent occurrence. F. R., a white woman, aged 45, admitted to St. Vincent's Hospital, Bridgeport, Conn., April 10, 1932, complained of sharp pains in the epigastrium which were intermittent, belching of gas, nausea, and a sour taste in the mouth. She stated that she had never vomited. She had been operated on fifteen years before for a ruptured ectopic pregnancy. Recovery was good. Eighteen years before, she had had malaria. Otherwise her past history was negative. The present condition began about four months before admission with a dull ache in the epigastrium and a sour taste in the mouth. This ache gradually became worse and finally sharp pain was experienced which was intermittent in character. Although at first the pain was confined to the epigastrium, it later radiated to the lower right quadrant. The pain had no definite relation to food; however, she
Book Review Women under the Influence The National Center on Addiction and Substance Abuse at Columbia University. 292 pp. Baltimore, Johns Hopkins University Press, 2006. $50 (cloth); $20 (paper). 0-8018-8227-3 (cloth); 0-8018-8228-1 (paper). Alcohol Problems in Adolescents and Young Adults: Epidemiology, Neurobiology, Prevention, and Treatment Edited by Marc Galanter. 456 pp. New York, Springer-Verlag, 2006. $49.95. 0-387-29215-2
Authors: Jack H. Mendelson
Journal: New England Journal of Medicine (2006)