tbuckley/cabot-search huggingface dataset:
Improved Preservation of Saphenous Vein Grafts by the Use of Glyceryl Trinitrate–Verapamil Solution During Harvesting
Authors: Nick Roubos, Franklin L. Rosenfeldt, Stephen M. Richards, Robert A.J. Conyers, Bruce B. Davis
Journal: Circulation (1995)

Background High-pressure distension during harvesting damages the saphenous vein (SV) and may contribute to subsequent coronary artery bypass graft (CABG) occlusion. Application of vasodilator agents to the SV during harvesting may reduce the need for high-pressure distension and improve graft quality. We tested the effects of a vasodilator solution containing glyceryl trinitrate and verapamil (GV) or the conventional agent papaverine (Pap) on the pressure necessary to overcome SV spasm and on the structure and biochemistry of the SV graft. Methods and Results Thirty-six patients undergoing CABG were randomly allocated to receive an application of either topical and intraluminal GV solution, topical Pap, or topical and intraluminal Ringer’s solution (untreated) to the SV during harvesting. The peak and mean pressures required to distend the vein were recorded. Samples of SV were taken for microscopy and biochemical analysis just before we performed the anastomosis. The percentage of endothelial coverage was calculated by area measurements of stained en face preparations of the vein intima. The results for peak pressures (mm Hg) were: untreated, 479.2±27.5; Pap, 384.8±29.0; and GV, 309.5±28.3 ( P &lt;.001, GV plus Pap versus untreated); and the results for mean pressures (mm Hg) were untreated, 136.2±9.6; Pap, 102.2±10.8; and GV, 98.0±8.3 ( P &lt;.01, GV plus Pap versus untreated). The results for endothelial cover (%) were: untreated, 43.7±7.0; Pap, 44.1±9.2; and GV, 68.7±7.0 ( P &lt;.05, GV versus Pap); and the results for ATP (nmol/g wet wt) were: untreated, 67.3±12.7; Pap, 112.0±19.4; and GV, 132.5±22.7 ( P &lt;.05, GV plus Pap versus untreated). Conclusions First, pharmacological treatment of SV during harvesting, especially with GV solution, allows the use of a lower distension pressure and reduces the breakdown of high-energy phosphates in the vein wall. Second, topical and intraluminal use of GV solution during vein harvesting improves endothelial coverage compared with the topical use of Pap or no pharmacological treatment.
Physicochemical and immunologic characterization of low‐molecular‐weight allergoids of <i>Dactylis glomerata</i> pollen proteins
Authors: TD Ćirković, MN Bukilica, MD Gavrovi, ZM Vuji, S Petrovi, RM Jankov
Journal: Allergy (1999)

Orchard grass (Dactylis glomerata) pollen proteins were chemically modified by means of acid anhydrides (maleic and succinic anhydride) to obtain low-molecular-weight allergoids. Chemical modification in both cases led to the replacement of one positive charge (epsilon amino group of Lys) by one negative charge, yielding proteins with changed physicochemical properties in comparison to the native orchard grass-pollen proteins.Physicochemical characterization of derivatives was done by gel chromatography, SDS-PAGE, and isoelectric focusing. To examine the IgE-binding properties of these derivatives, we carried out immunoblotting. To examine the ability of derivatives to induce IgG production, we immunized rabbits. Skin prick testing with the allergoids was performed on 15 individuals allergic to orchard grass pollens and on two healthy subjects.It was shown that the modified proteins retain their original molecular weights, but change pI to more acidic values. In the case of allergoids, a strong reduction in IgE binding was found. Immunization of rabbits with allergoids showed that the derivatives retain the ability to induce IgG production, and that the antisera obtained in such a way react to native (unmodified) extract. The ability of derivatives to induce allergic reaction was significantly reduced. The patients (86.6%) included in our study exhibited less than 50% of native extract response. Among them, 53.3% had no response to one or both allergoids.These modification procedures yield allergoids with a reduced allergenic activity and preserved immunogenic potential suitable for use in immunotherapy.
Long-Term Efficacy of Two Cycles of BEP Regimen in High-Risk Stage I Nonseminomatous Testicular Germ Cell Tumors with Embryonal Carcinoma and/or Vascular Invasion*1
Authors: Christine Chevreau, Catherine Mazerolles, Michel Soulié, Marie-Hélène Gaspard, Loı̈c Mourey, Louis Bujan, Pierre Plante, Pascal Rischmann, Jean-Marc Bachaud, Bernard Malavaud
Journal: European Urology (2004)
Lymphatic Dysfunction in the Apparently Clinically Normal Contralateral Limbs of Patients With Unilateral Lower Limb Swelling
Authors: Katherine M. Burnand, Daphne M. Glass, Peter S. Mortimer, Adrien Michael Peters
Journal: Clinical Nuclear Medicine (2011)

Purpose: To determine how often lymphatic dysfunction is bilateral when, clinically, lymphedema appears unilateral. Methods: Lymphoscintigraphy was performed after subcutaneous Tc-99m-nanocolloid injection in the first webspaces of both feet. The percentage of injected radioactivity accumulating in the ilioinguinal regions was recorded in dedicated images separately acquired at 60 and 180 minutes after injection. Results: Within a consecutive series of 204 patients, 74 had unilateral clinical lymphedema of whom 68 had abnormal scintigraphy. Of these 68 patients, 46 had unilateral abnormal scintigraphy affecting the clinically abnormal limb, but 20 patients had bilateral abnormal scintigraphy and 2 had unilateral abnormal scintigraphy in the clinically unaffected limb. Thus, 32% (22/68) of patients in whom clinical lymphedema appeared to be unilateral, nevertheless, had abnormal scintigraphy in the clinically normal limb. Twenty-nine patients had no clinical evidence of lymphedema in either limb and were scintigraphically normal bilaterally. Mean ilioinguinal nodal accumulation at 180 minutes in the 44 limbs of 22 of these clinically and scintigraphically normal patients (dedicated ilioinguinal imaging was not performed in all patients) was 13.1% (standard deviation, 8.8%), higher (P = 0.02) than the mean value of 9.3% (standard deviation, 5.0%) in the clinically and scintigraphically normal contralateral limbs of 39 patients with unilateral clinical lymphedema. Conclusions: In the presence of unilateral lymphedema, the contralateral limb is often also abnormal. On lymphoscintigraphy, therefore, care should be taken before diagnosing unilateral lymphatic dysfunction. Quantification should be included in routine lymphoscintigraphy, as reduced ilioinguinal nodal accumulation may be the only apparent abnormality.
Family Outbreak of Primary Atypical Pneumonia Associated with the Eaton Agent
Authors: Richard T. Johnson, M. Katherine Cook, Robert M. Chanock, Edward L. Buescher
Journal: New England Journal of Medicine (1960)

THE possible role of the Eaton agent in the etiology A of primary atypical pneumonia is presented in a previous report.1 A family outbreak of primary atypical pneumonia, involving one of us (R.T.J.), is described to exemplify the clinical manifestations, the antibody response to the Eaton agent as measured by the fluorescent-antibody method and the inconsistency in development of cold and Streptococcus MG agglutinins during infection and convalescence.Case ReportsThe household unit consisted of 2 adults, a medical officer working in a virology laboratory and his wife, who was employed as a school teacher. Neither had known exposure to . . .
Induction of major histocompatibility complex antigens within the myocardium of patients with active myocarditis: A nonhistologic marker of myocarditis
Authors: Ahvie Herskowitz, Aftab Ahmed-ansari, David A. Neumann, William E. Beschorner, Noel R. Rose, Lisa M. Soule, C. Lynne Burek, Kenneth W. Sell, Kenneth L. Baughman
Journal: Journal of the American College of Cardiology (1990)
A systematic analysis of recombination activity and genotype-phenotype correlation in human recombination-activating gene 1 deficiency
Authors: Yu Nee Lee, Francesco Frugoni, Kerry Dobbs, Jolan E. Walter, Silvia Giliani, Andrew R. Gennery, Waleed Al-Herz, Elie Haddad, Francoise LeDeist, Jack H. Bleesing, Lauren A. Henderson, Sung-Yun Pai, Robert P. Nelson, Dalia H. El-Ghoneimy, Reem A. El-Feky, Shereen M. Reda, Elham Hossny, Pere Soler-Palacin, Ramsay L. Fuleihan, Niraj C. Patel, Michel J. Massaad, Raif S. Geha, Jennifer M. Puck, Paolo Palma, Caterina Cancrini, Karin Chen, Mauno Vihinen, Frederick W. Alt, Luigi D. Notarangelo
Journal: Journal of Allergy and Clinical Immunology (2013)
Safety and Efficacy of Repaglinide in Type 2 Diabetic Patients With and Without Impaired Renal Function
Authors: Christoph Hasslacher
Journal: Diabetes Care (2003)

OBJECTIVE—To evaluate the influence of renal impairment on the safety and efficacy of repaglinide in type 2 diabetic patients. RESEARCH DESIGN AND METHODS—This multinational, open-label study comprised a 6-week run-in period, continuing prestudy antidiabetic medication, followed by a titration period (1–4 weeks) and a 3-month maintenance period. Patients with normal renal function (n = 151) and various degrees of renal impairment (n = 130) were treated with repaglinide (maximal dose of 4 mg, three times daily). Safety and efficacy assessments were performed at baseline (end of run-in) and at the end of study treatment. RESULTS—The type and severity of adverse events during repaglinide treatment were similar to the run-in period. The number of patients with adverse events was not significantly related to renal function during run-in or repaglinide treatment. Percentage of patients with hypoglycemic episodes increased significantly (P = 0.007) with increasing severity of renal impairment during run-in but not during repaglinide treatment (P = 0.074). Metabolic control (HbA1c and fasting blood glucose) with repaglinide was unchanged from that on previous antidiabetic medication. Final repaglinide dose tended to be lower for patients with severe and extreme renal impairment than for patients with less severe renal impairment or normal renal function (P = 0.032). CONCLUSIONS—Repaglinide has a good safety and efficacy profile in type 2 diabetic patients complicated by renal impairment and is an appropriate treatment choice, even for individuals with more severe degrees of renal impairment.
PREVENTION OF TUBERCULOSIS IN BABES BORN OF TUBERCULOUS PARENTS.
Authors: CLIFTON SCOTT
Journal: JAMA (1901)

In the presentation of this subject, I desire to present two cases and from them draw my conclusions and upon them base my arguments.  Case 1.  —Mrs. A. H., a young woman, married at 19 years of age, had suffered with chronic pulmonary tuberculosis for five years previous, the existence of which, she and her mother denied. During the first two years of her married life she had frequent mild attacks of hemoptysis. She continually consulted her old, family physician, a homeopath, for these troubles, until Oct. 15, 1896, when she had a severe hemorrhage and I was called in haste. By putting her in bed and applying ice to the chest, the hemorrhage was controlled. I was then invited to take charge, of the case.  I found a pulse of 96, temperature of 101, some moist rales in the lungs, quite marked emaciation; cough was not serious, except a
Abstract 2846: Safety and efficacy of a genetic vaccine targeting telomerase <i>plus</i> chemotherapy for the therapy of canine B-cell lymphoma .
Authors: Alessandra Gavazza, George Lubas, Arthur Fridman, Daniela Peruzzi, Joseph A. Impellizeri, Laura Luberto, Emanuele Marra, Giuseppe Roscilli, Gennaro Ciliberto, Luigi Aurisicchio
Journal: Cancer Research (2013)

Abstract Background: We have recently shown that a genetic vaccine targeting dog telomerase (dTERT) and based on Ad/DNA-EGT technology can induce strong cell-mediated immune responses against this tumor antigen and increase overall survival of dogs affected by B-cell lymphosarcoma (LSA) in comparison with historical controls when combined with COP chemotherapy regimen. Objectives: Here, we have conducted a double arm clinical trial with an extended number of LSA patients, measured the antigen-specific immune response and evaluated potential toxic effects of the immunotherapy along with a follow up of patients survival for three years and half. Methods: The immune response was measured by ELISPOT. The expression of dTERT was quantified by quantitative PCR. Changes in hematological parameters, local/systemic toxicity or organic dysfunction and fever were monitored over time during the treatment. Results: dTERT-specific cell mediated immune responses were induced in almost all treated animals No adverse effects were observed in any dog patient that underwent treatment. The overall survival time of vaccine/COP treated dogs was significantly increased over the COP-only treated cohort (&amp;gt;76.1 vs 29.3 weeks, respectively, p&amp;lt;0.0001). There was a significant association between dTERT expression levels and overall survival (OS) among vaccinated patients. Conclusions and Clinical relevance: Ad/DNA-EGT-based cancer vaccine against dTERT in combination with COP chemotherapy is safe and significantly prolongs the survival of LSA canine patients. These data confirm the therapeutic efficacy of dTERT vaccine and support the evaluation of this approach in human trials and for other cancer types. Citation Format: Alessandra Gavazza, George Lubas, Arthur Fridman, Daniela Peruzzi, Joseph A. Impellizeri, Laura Luberto, Emanuele Marra, Giuseppe Roscilli, Gennaro Ciliberto, Luigi Aurisicchio. Safety and efficacy of a genetic vaccine targeting telomerase plus chemotherapy for the therapy of canine B-cell lymphoma . [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2846. doi:10.1158/1538-7445.AM2013-2846
Defibrillation of 100 kg calves with asymmetrical, bidirectional, rectangular pulses
Authors: J. C SCHUDER, W. C McDANIEL, H. STOECKLE
Journal: Cardiovascular Research (1984)

The effectiveness in reversing ventricular fibrillation of 30 s duration of asymmetrical, bidirectional, rectangular waveforms in which the lagging half-cycle has the same duration but lower amplitude than the leading portion of the waveform was evaluated in a 2160-episode study involving anaesthetised calves. An additional 480-episode auxiliary study involved the interlacing of unidirectional and bidirectional wave episodes. The leading half-cycles of the 18 bidirectional waveforms evaluated were 35 A at 8 and 16 ms, 50 A at 4 and 8 ms, and 70 A at 2 and 4 ms. Associated with each of the six leading half-cycle configurations were lagging half-cycles having reverse current levels of l/8th, l/4th, and 1/2 of the leading half-cycle current amplitudes. Six waveforms were successful in 97% or more of the transthoracic episodes. Of these, three were 100% successful. Our data, when combined with those from earlier unidirectional and symmetrical, bidirectional, rectangular waveform studies, suggest that a broad category of bidirectional rectangular shocks are superior to the most favourable unidirectional rectangular shock.
Virus-induced airway hyperresponsiveness in guinea pigs is related to a deficiency in nitric oxide.
Authors: G Folkerts, H J van der Linde, F P Nijkamp
Journal: Journal of Clinical Investigation (1995)

Intratracheal inoculation of parainfluenza type 3 virus to guinea pigs induces a marked increase in airway responsiveness in vivo and in vitro. In spontaneously breathing anesthetized guinea pigs inhalation of an aerosol containing the nitric oxide (NO) precursor L-arginine (2.0 mM) completely prevented the virus-induced airway hyperresponsiveness to histamine. In addition, perfusion of L-arginine (200 microM) or the direct NO-donor S-nitroso-N-acetyl-penicillamine (SNAP, 1 microM) through the lumen of tracheal tubes from infected animals prevented the increase in airway responsiveness to histamine or the cholinoceptor agonist methacholine. The NO synthase inhibitor N omega-nitro-L-arginine methyl ester (L-NAME, 120 microM) did not further increase the virus-induced airway hyperresponsiveness. In additional experiments, NO was measured with an Iso-NO nitric oxide meter and sensor. Stimulation of control tissues in vitro with histamine (10(-3) M) resulted in a contraction with a simultaneous release of NO (44.5 +/- 5.4 nM). The release of NO was markedly reduced by 75% (P < 0.01, 11.4 +/- 3.1 nM) in tracheas from virus-infected animals that demonstrated enhanced contractile responses. Preincubation of tissues from virus-treated guinea pigs with L-arginine (200 microM) completely prevented the enhanced contraction and simultaneously returned the NO production to control values (51.2 +/- 3.4 nM). An NO deficiency might be causally related to the development of airway hyperresponsiveness after a viral respiratory infection.
Effect of sodium aurothiomalate on carrageenan induced inflammation of the air pouch in mice.
Authors: Y M Sin, M K Wong
Journal: Annals of the Rheumatic Diseases (1992)

Acute inflammation was induced by injecting carrageenan into a 6 day old air pouch in mice. Sodium aurothiomalate was then given twice to each of three groups of mice via different routes. It was found that the mice injected intravenously with sodium aurothiomalate showed the most striking reduction in the number of exudate leucocytes in the inflammatory cavity, although the amount of gold found in their inflamed pouch lining tissue was the least. The amount of gold in plasma was highest in the mice injected intravenously with sodium aurothiomalate and the least amount of gold was found in the mice injected directly into the air pouch with sodium aurothiomalate. The amount of gold in the inflamed pouch lining tissue reached its peak at 24 hours after injection and a significant decrease of exudate leucocytes was only seen 24 and 72 hours after injection. The amount of gold in the exudate fluid was negligible at all the times studied. No significant difference was noted in the degree of inflammatory suppression when increasing doses of sodium aurothiomalate were injected into the air pouch. These findings show that there is no direct correlation between the gold concentration in the inflamed tissue and suppression of the inflammatory reactions in the cavity. Chemotactic and phagocytic analysis of leucocytes in the exudate showed that there was a significant suppression of the neutrophil activities in all the mice treated with sodium aurothiomalate. It is therefore concluded that the significant reduction in the number of exudate leucocytes at the carrageenan induced inflammatory site after treatment with sodium aurothiomalate is most likely due to the direct action of gold on the functional activities of circulating neutrophils.
Renal Concentrating Mechanisms.
Authors: R. W. Berliner
Journal: Annals of Internal Medicine (1962)

s1 April 1962Renal Concentrating Mechanisms.R. W. Berliner, M.D.R. W. Berliner, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-56-4-696_4 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe formation of concentrated urine is unique, not in the transport processes which are involved, but in the functional organization of these processes. The underlying mechanism appears to be the same sodium transport process which has been studied in many other tissues and organs. This process, together with the unusual anatomical distribution of mammalian nephron segments into cortex and medulla and the varying permeability of these segments to water, makes possible the formation of markedly hypotonic or hypertonic urine as the situation may require. The salt transport most pertinent to dilution and concentration is that which occurs in the ascending... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: R. W. Berliner, M.D.Affiliations: Bethesda, Md. (BS) PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 April 1962Volume 56, Issue 4Page: 696-697KeywordsAttentionNephronsSaltsSodiumUrine ePublished: 1 December 2008 Issue Published: 1 April 1962 PDF downloadLoading ...
Adequate Enzyme Replacement After Total Pancreatectomy
Authors: André Robert
Journal: JAMA (1989)

<h3>To the Editor.—</h3> Two years ago, at age 60 years, I developed multifocal adenocarcinoma of the pancreas. The only signs were some abdominal discomfort 2 to 3 hours after meals, two bowel movements per day instead of one, and softer stools. They persisted for about 3 weeks. Then, jaundice (without pain) was noticed by a colleague 1 week prior to hospitalization. The surgical treatment consisted of Whipple's operation (total pancreatectomy, duodenectomy, gastrojejunostomy, splenectomy, and cholecystectomy). Quite a job. This was followed by chemotherapy (fluorouracil, doxorubicin [Adriamycin], and mitomycin) and abdominal radiation. The pancreatectomy produced instant type I diabetes and total lack of digestive enzymes. To compensate for the latter, I first took three capsules of pancrelipase (Pancrease) before each meal. Later on, I switched to pancreatin and then Entolase HP. The insulin doses have been 20 U of insulin zinc suspension (Lente insulin) in the morning and 7 U of
TRAUMATIC STRICTURE OF THE VAGINA.
Authors: BAYARD HOLMES
Journal: JAMA (1896)

A bride of three days consulted me on account of unendurable pain at the first attempt at sexual intercourse. She was a young woman of apparently perfect health. She said she had never been sick in her life and that she had worked constantly as a nurse for several years after taking training in a small hospital. In her girlhood she had fallen on a picket fence and injured the vagina and bladder, but she believed she had entirely recovered from that injury. At each menstrual period she bloated some but had no pain. There was always a rather long flow, sometimes lasting six or eight days. Clots were never seen. There had always been some leucorrhea. On making an examination the vagina was found closed just beyond the remnants of the hymen. A large tumor could be felt through the rectum about an inch and a half behind this
Glucomannan and risk of oesophageal obstruction.
Authors: D A Henry, A S Mitchell, J Aylward, M T Fung, J McEwen, A Rohan
Journal: BMJ (1986)

When all results were considered together the urine tube immunoassay (Model) gave 100% sensitivity and specificity as well as 100% predictive values for normal and abnormal results.The serum tube enzyme linked immunosorbent assay, while showing 100% sensitivity, was less specific, and the dipsticks were intermediate in value.
Autotransfusion for surgery: A comeback?
Authors: May Annexton
Journal: JAMA (1978)

Replacement of blood loss during surgery with the patient's own blood—certainly not a new idea— currently is attracting increased attention from anesthesiologists and surgeons. Why the second look? First, autotransfusion gives the patient the best blood product he can use: his own. Second, use of autologous blood reduces demand on local and national blood reserves. Several reports on intraoperative autotransfusion were presented at the recent Chicago meeting of the American Society of Anesthesiologists. Eric Furman, MB, BCh, of the Department of Anesthesiology, Children's Orthopedic Hospital and Medical Center and the University of Washington School of Medicine, Seattle, employs the hemodilution technique of autotransfusion. A predetermined amount of blood is withdrawn from the patient at the time of surgery and replaced with an electrolyte solution. When surgery is completed, the blood is returned. An intravenously administered diuretic promotes the elimination of the electrolyte solution. The procedure is indicated, Furman states, in