tbuckley/cabot-search huggingface dataset:
STUDIES ON ANTIBODY-PRODUCING CELLS
Authors: Fred G. Gudat, T. N. Harris, Susanna Harris, Klaus Hummeler
Journal: The Journal of Experimental Medicine (1971)

Mice injected with sheep RBC and then, 4 days later with thymidine-3H, were sacrificed on the day of thymidine-3H injection or 1 or 2 days later. Hemolytic antibody plaque preparations were made of cells from the draining lymph nodes by a thin-plating procedure permitting collection of isolated PFC for electron microscopic examination and radioautography. Of cells obtained on the day of thymidine-3H injections, 65% of the labeled PFC were in the lymphocytic category, in comparison with 13% found previously in the entire population of such cells. The remaining 35% were plasmablasts in early stages of differentiation. Cells obtained 1 day after the thymidine-3H injections showed a shift to a majority of labeled cells in the plasmacytic category. Also, the plasmablasts were substantially more differentiated than those of the previous day, and some mature plasma cells were now seen. The labeled PFC obtained on day 2 gave no indication of further differentiation. Cells of rabbit lymph nodes labeled in vitro with thymidine-3H showed a range of labeled PFC. The majority were in the plasmacytic category, including some mature plasma cells. The data from the experiments with in vivo labeling suggest a direct differentiation from antibody-synthesizing lymphocytes to plasma cells. Further, the in vivo experiments indicated that differentiation from nascent lymphocyte to plasma cell could be essentially completed within 1 day.
Heavy drinking costs the NHS 1.7bn a year, says report
Authors: R. Dobson
Journal: BMJ (2003)

Treating illnesses and injuries caused by alcohol misuse is costing the NHS up to £1.7bn ($2.8bn; €2.4bn) a year, according to a new report.

Heavy drinking is resulting in an increasing number of premature deaths, health problems, social disorder, and injuries, says the report, which says that in the United Kingdom up to 40% of men's drinking sessions can be classed as binge drinking.

But the total costs, including the costs of absenteeism, alcohol related crime, and human suffering, may be as high as £20bn–almost three times the estimated £7bn in excise duty on alcohol that the government receives each year.

The …
748 BLOOD LIPIDOMIC PROFILING OF HEPATOCELLULAR CARCINOMA IN HUMAN AND ANIMAL STUDIES IDENTIFIES LYSOPHOSPHATIDYLCHOLINE (24, 0, 0) AS A DISCRIMINATORY BIOMARKER
Authors: M.I.F. Shariff, M.R. Lewis, E.J. Want, H.C. Keun, F. Mohamed, R. Jalan, N.G. Ladep, M.M.E. Crossey, S.A. Khan, E. Holmes, S.D. Taylor-Robinson
Journal: Journal of Hepatology (2012)
Stem cell mobilization in patients with newly diagnosed multiple myeloma after lenalidomide induction therapy
Authors: F Cavallo, S Bringhen, G Milone, D Ben-Yehuda, A Nagler, E Calabrese, N Cascavilla, V Montefusco, B Lupo, A M Liberati, C Crippa, F Rossini, R Passera, F Patriarca, A M Cafro, P Omedè, A M Carella, J Peccatori, L Catalano, T Caravita, P Musto, M T Petrucci, M Boccadoro, A Palumbo
Journal: Leukemia (2011)
Influence of inflation and atelectasis on the hypoxic pressor response in isolated dog lung lobes
Authors: E. J. QUEBBEMAN, C. A. DAWSON
Journal: Cardiovascular Research (1976)

Journal Article Influence of inflation and atelectasis on the hypoxic pressor response in isolated dog lung lobes Get access EDWARD J. QUEBBEMAN, EDWARD J. QUEBBEMAN From the Research Service, Veterans Administration Center, Wood (Milwaukee), Wisconsin 53193, USA and the Departments of Surgery and Physiology, The Medical College of Wisconsin, Milwaukee, Wisconsin 53233, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar CHRISTOPHER A. DAWSON CHRISTOPHER A. DAWSON From the Research Service, Veterans Administration Center, Wood (Milwaukee), Wisconsin 53193, USA and the Departments of Surgery and Physiology, The Medical College of Wisconsin, Milwaukee, Wisconsin 53233, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 10, Issue 6, November 1976, Pages 672–677, https://doi.org/10.1093/cvr/10.6.672 Published: 01 November 1976
Sonic Hedgehog Expands Diaphyseal Trabecular Bone Altering Bone Marrow Niche and Lymphocyte Compartment
Authors: Maija Kiuru, Chisa Hidaka, Ralf-Harto Hubner, Jason Solomon, Anja Krause, Philip L Leopold, Ronald G Crystal
Journal: Molecular Therapy (2009)

Bone marrow contains distinct microenvironments that regulate hematopoietic stem cells (HSCs). The endosteal HSC niche includes osteoblasts, mineral, and extracellular matrix proteins that interact through various molecular signals to control HSCs. Sonic hedgehog (Shh) is a morphogen involved in the regulation of skeletal development and hematopoiesis, but the effects of Shh on bone in relation to the HSC niche are not well understood. We demonstrate that systemic overexpression of Shh in mice increases osteoblast number with the resultant formation of new trabeculae in the femoral diaphysis. Suggestive of a functional change in the hematopoietic niche, numbers of Lin(-) Sca-1(+) c-Kit(+) cells with hematopoietic progenitor function expand, although cells with in vivo repopulating capacity in the wild-type environment do not increase. Instead, Shh mediates a decrease in number of bone marrow lymphocytes accompanied by a decreased expression of stromal-derived growth factor 1 (SDF-1) and a decrease in Flk2-expressing Lin(-) Sca-1(+) c-Kit(+) cells, indicating a modulation of early lymphopoiesis. This is caused by a microenvironment-induced mechanism as Shh treatment of bone marrow recipients, but not donors, results in a dramatic depletion of lymphocytes. Together, these data suggest that Shh mediates alterations in the bone marrow hematopoietic niche affecting the early lymphoid differentiation.
Hb regina or α<sub>2</sub>β<sub>2</sub>96(FG3)LEU → VAL: A high oxygen affinity variant discovered by cation‐exchange HPLC
Authors: R. Devaraj, J. B. Wilson, T. H. J. Huisman
Journal: American Journal of Hematology (1985)

Abstract Results are reported of studies of the hemoglobin from one member of a Canadian family with a mild erythrocytosis. This variant, which accounted for about 40% of the hemoglobin, could be separated from Hb A and Hb A 2 by cation‐exchange chromatography. Micro methodology allowed the characterization of a Leu → Val replacement at position β96(FG3). Hb Regina has an increased oxygen affinity, which adequately explains the hematological observations.
Myocardial morphometrics in pressure overload left ventricular hypertrophy and regression
Authors: E. A BREISCH, A. A BOVE, S. J PHILLIPS
Journal: Cardiovascular Research (1980)

The ultrastructural effects of pressure overload hypertrophy and its subsequent regression were studied in three regions of the left ventricular myocardium of ferrets. Quantitative analysis of electron micrographs was carried out by computer analysis. Four animals were studied 14 days after a 70% constriction of the ascending aorta. Five animals were similarly constricted for 14 days but were studied 6 weeks after the aortic band had been removed. Five animals served as controls. 70% aortic constriction produced a 45% increase in LV mass. Ultrastructural examination showed no qualitative difference between hypertrophy and control groups. Quantitative analysis showed an increased mass of mitochondria and myofibrils, but the fractional cell volume of mitochondria and myofibrils was unchanged in hypertrophy as was the mitochondria/myofibril ratio. In the hypertrophy group, regional differences in fractional cell volume of myofibrils were noted. After 6 weeks of debanding, left ventricular mass was not different from controls. No qualitative ultrastructural changes were found in the regression group, and fractional cell volume of mitochondria and myofibrils showed no difference between regression and controls. However, regional differences were again noted in the fractional cell volume of myofibrils for regression animals. The mitochondria / myofibril ratio of regression and control groups was not different, nor was the mitochondrial and myofibrillar mass different. For this hypertrophy model normal ultrastructure is maintained in hypertrophy and regression. Quantitative morphometry suggests that a normal balance between mitochondria and myofibrils is maintained in the myocardium after 14 days of hypertrophy and 6 weeks of recovery from hypertrophy.
GONORRHEA AS A FACTOR IN PUERPERAL FEVER.
Authors: ALBERT H. BURR
Journal: JAMA (1898)

The methods which have revolutionized modern surgery as the direct results of the teachings of Lister have been more or less efficiently carried out in the practice of modern obstetrics. The new science of bacteriology and the accumulating evidences of the pathogenic nature and far-reaching effects of certain micro-organisms have invested the subject of the prophylactic, aseptic and antiseptic protection of the parturient with increasing interest and importance. Notwithstanding the advancements along these lines, the query arises, why is there not greater freedom from septic complications and fatal issues in the service of those who carry out the most approved measures in the practice of midwifery? In other words, are there not frequently sources of danger within the mother herself, antedating childbed, which ordinary methods fail to avert? I desire to emphasize the importance of these vital questions. Since the discovery of the gonococcus which Neisser announced as the specific
UK population enjoys a healthier older age
Authors: M. Limb
Journal: BMJ (2012)

More people in the UK are staying healthy into later life, according to figures from the Office for National Statistics (ONS), but disparities between countries are concerning public health specialists.

Between the periods 2005-07 and 2008-10 there were “significant increases” of between 2.1 and 3.5 years in healthy life expectancy for males and females at birth in the UK as a whole. The proportion of life spent in very good or good general health is increasing in England and Wales but, on the whole, falling in Scotland and Northern Ireland, says the ONS.

Ed Jessop, vice president of the Faculty of Public Health, called for action to tackle the root causes of health inequalities, saying, “The gap between the …
Immunochemical characterization of Russian thistle (<i>Salsola kali</i>) pollen extracts. Purification of the allergen Sal k 1
Authors: J. Carnés, E. Fernández‐Caldas, A. Marina, C. Alonso, C. Lahoz, C. Colás, A. Lezaun
Journal: Allergy (2003)

Salsola kali (Russian thistle) is a weed which belongs to the Chaenopodiacea family. It is widely distributed along the coasts of Europe, North Africa, USA and Australia. The objectives of this study were to study the allergenic composition of S. kali pollen and to purify an important allergen from the pollen extracts of this plant.A population of 66 individuals with specific IgE-mediated allergic symptoms and positive skin tests to S. kali were included in the study. Specific IgE to S. kali was determined by direct enzyme-linked immunosorbent assay (ELISA). The antigenic and allergenic profile of S. kali was evaluated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focussing (IEF) and immunoblot. Allergen purification was conducted by preparative SDS-PAGE. The allergenicity of the protein was evaluated by skin testing, direct ELISA, ELISA inhibition and immunoblots.Specific IgE to S. kali was detected in 39 of the 66 individuals (59%). An allergen with a molecular weight of approximately 43 kDa was purified. This allergen was termed Sal k 1. A partial sequencing was obtained and no homology was found with other known proteins/allergens. The allergenicity of Sal k 1 was tested in vitro and in vivo. Of the 39 individuals with a positive specific IgE determination to S. kali, 26 (66.6%) had detectable specific IgE to Sal k 1. Twenty of these 39 individuals were skin-prick tested with the purified allergen (0.5 mg/ml) and all of them had a positive skin test to the purified allergen. Ten additional individuals, used as negative controls, had a negative response.Sal k 1, an important allergen of S. kali, is recognized, in vitro, by approximately 67% of the patients sensitized to S. kali. Twenty patients with a positive skin test to a standardized S. kali extract had a positive reaction to the purified allergen.
RESUSCITATION OF NEWBORN INFANTS
Authors: J. P. Greenhill
Journal: JAMA (1953)

<h3>To the Editor:</h3> —InThe Journal, May 16, 1953, there is an article "Resuscitation of the Asphyxiated Newborn Infant." The first sentence reads, "It is generally agreed that responsibility for resuscitation of the newborn infant belongs to the anesthesiologist." May I ask, agreed by whom? Surely obstetricians have not agreed on this. Would the authors of the article have the anesthetist, who is keeping the mother asleep with an inhalation anesthetic, stop administering the anesthetic and resuscitate the baby for the obstetrician, or would they perhaps recommend that there be two anesthetists available in every delivery room, one to care for the mother and the other for the baby? Anesthetists (at least some) are not the only ones who consider it their prerogative to resuscitate newborn infants. The officials of the Chicago health department are of the firm opinion that a pediatrician should be in the labor room at the
Does the effect of immunotherapy last after termination of treatment?
Authors: H. Mosbech, O. ØTerballe
Journal: Allergy (1988)

In a previous study, 39 adults with grass pollen allergy were hyposensitized for approximately 2 1/2 years. Treatment was performed in a double-blind fashion with extract made from timothy grass--either Alutard SQ 20-component extract or a purified 2-component extract, including only the two major allergens Phl p V and VI. Standardized symptom + medicine scores and challenge tests demonstrated a clinical effect, most markedly in the group receiving 20-component extract. Six years after termination of treatment, 38 patients could be approached and 16 in each group were examined and repeated symptoms scoring during the subsequent season. When adjustment for variations in pollen counts were made, medicine + symptom scores stayed low during the follow-up period. Specific IgE-antibodies against timothy showed an increase to initial values during the same period, whereas total IgE antibodies remained low. Skin prick test reactions with timothy allergen tended to increase but were still smaller than before treatment. Retrospectively, the patients reported symptoms to have stabilized or even further decreased after termination of treatment, with no significant difference between groups. In conclusion, the clinical effect was still present more than 6 years after termination of treatment. Some in vitro parameters tended to return to pretreatment level. The spontaneous course of the disease in non-hyposensitized patients was not investigated.
European guidelines for quality assurance in colorectal cancer screening and diagnosis. First Edition – Management of lesions detected in colorectal cancer screening
Authors: R. Steele, C. Pox, E. Kuipers, G. Minoli, R. Lambert
Journal: Endoscopy (2012)

Multidisciplinary, evidence-based guidelines for quality assurance in colorectal cancer screening and diagnosis have been developed by experts in a project coordinated by the International Agency for Research on Cancer. The full guideline document covers the entire process of population-based screening. It consists of 10 chapters and over 250 recommendations, graded according to the strength of the recommendation and the supporting evidence. The 450-page guidelines and the extensive evidence base have been published by the European Commission. The chapter on management of lesions detected in colorectal cancer screening includes 32 graded recommendations. The content of the chapter is presented here to promote international discussion and collaboration by making the principles and standards recommended in the new EU Guidelines known to a wider professional and scientific community. Following these recommendations has the potential to enhance the control of colorectal cancer through improvement in the quality and effectiveness of the screening process, including multi-disciplinary diagnosis and management of the disease.
Nitroglycerin Tolerance in Human Vessels
Authors: Peter R. Sage, Ivan S. de la Lande, Irene Stafford, Catherine L. Bennett, George Phillipov, John Stubberfield, John D. Horowitz
Journal: Circulation (2000)

Background —The basis for progressive attenuation of the effects of organic nitrates during long-term therapy (nitrate tolerance) remains controversial; proposed mechanisms include impaired nitrate bioconversion resulting in decreased release of nitric oxide (NO) from nitrates and/or increased NO clearance through a reaction with incrementally generated superoxide (O 2 – ). Methods and Results —Patients undergoing elective coronary artery bypass were randomized to receive 24 hours of intravenously infused nitroglycerin (NTG; nitrate group) or no nitrate therapy (control group). Discarded segments of the internal mammary artery and saphenous vein were used to examine (1) vascular responsiveness to NTG, sodium nitroprusside, and the calcium ionophore A23187; (2) bioconversion of NTG to 1,2- and 1,3-glyceryl dinitrate; and (3) the generation of O 2 – . Responses to NTG were reduced 3- to 5-fold in vessels from the nitrate group compared with control vessels ( P &lt;0.01 for both types of segments), whereas responses to sodium nitroprusside and A23187 were unchanged. Tissue content of 1,2-glyceryl dinitrate was lower ( P =0.012) in the saphenous veins from the nitrate group than in those from the control group. O 2 – generation was greater ( P &lt;0.01) in internal mammary artery samples from the nitrate group than in those from the control group. However, incremental O 2 – generation induced by an inhibitor of superoxide dismutase did not affect NTG responses. Conclusions —NTG tolerance in patients with coronary artery disease is nitrate-specific and is associated with evidence of impaired NTG bioconversion. Tolerance was associated with incremental O 2 – generation, but short-term elevation of O 2 – did not affect NTG responsiveness, suggesting increased NO clearance by O 2 – has a minimal contribution to tolerance.
Television documentary highlights epilepsy
Authors: Phil Gunby
Journal: JAMA (1985)

This is National Epilepsy Month. A documentary—introduced at the American Neurological Association meeting in Chicago last month—will be shown on the Lifetime Cable Network (which telecasts the "AMA Medicine Today" program on "Doctors' Sunday" each week) Nov 6 at 11 am Eastern time, Nov 16 at 8 pm, and Nov 22 at 3:30 pm. It will be telecast for a final time Dec 2 at 8 pm, after which viewers, including physicians, will have an opportunity to telephone in questions. The televised documentary is entitled "Epilepsy: Quality of Life." It is a key feature of a program designed to help improve quality of life for patients with epilepsy. The physician and public awareness program is being conducted by the Epilepsy Foundation of America, which says that about 2 million Americans have epilepsy. Emphasis now is on quality of life, as well as control of seizures, clinicians in this field say
Memory CD4+ T cells do not induce graft-versus-host disease
Authors: Britt E. Anderson, Jennifer McNiff, Jun Yan, Hester Doyle, Mark Mamula, Mark J. Shlomchik, Warren D. Shlomchik
Journal: Journal of Clinical Investigation (2003)

Graft-versus-host disease (GVHD) remains a major cause of morbidity and mortality in allogeneic stem cell transplantation (alloSCT). Donor T cells that accompany stem cell grafts cause GVHD by attacking recipient tissues; therefore, all patients receive GVHD prophylaxis by depletion of T cells from the allograft or through immunosuppressant drugs. In addition to providing a graft-versus-leukemia effect, donor T cells are critical for reconstituting T cell–mediated immunity. Ideally, immunity to infectious agents would be transferred from donor to host without GVHD. Most donors have been exposed to common pathogens and have an increased precursor frequency of memory T cells against pathogenic antigens. We therefore asked whether memory CD62L–CD44+ CD4+ T cells would induce less GVHD than unfractionated or naive CD4+ T cells. Strikingly, we found that memory CD4 cells induced neither clinical nor histologic GVHD. This effect was not due to the increased number of CD4+CD25+ regulatory T cells found in the CD62L–CD44+ fraction because memory T cells depletion of these cells did not cause GVHD. Memory CD4 cells engrafted and responded to antigen both in vivo and in vitro. If these murine results are applicable to human alloSCT, selective administration of memory T cells could greatly improve post-transplant immune reconstitution.
Effect of Various Adrenal Steroids on the Electrocardiogram of Adrenalectomized Dogs
Authors: JOHN P. DAVANZO, H. C. CROSSFIELD, W. W. SWINGLE
Journal: Circulation Research (1961)

The effects of large amounts of various adrenal steroids on the electrocardiogram of the adrenalectomized dog are described. The changes which occurred most frequently fell into 2 major groups: (1) those following excess of mineralo-corticoids, e.g., desoxycorticosterone and aldosterone, and (2) those appearing after gluco-corticoid administration. A characteristic type of diphasic T wave frequently followed treatment with hydrocortisone and its analogs, and its correction was hastened by substituting mineralo-corticoid therapy. The 3 analogs of hydrocortisone (dexamethasone, prednisolone and triam$$$inolone) induce similar electrocardiographic patterns and after prolonged administration alter the P-Q interval in varying degrees, but more frequently produce a diphasic T. Dexamethasone preserves the normal pattern longer than the other steroids tested.
Diagnosis of Squamous Cell Carcinoma Metastasis With 18F-FDG PET/CT in Stoma After Percutaneous Endoscopic Gastrostomy
Authors: Erwan de Monès, Vincent Castetbon, Laurence Digue, Bruno Baltazart, Philippe Fernandez
Journal: Clinical Nuclear Medicine (2013)

Patients undergoing percutaneous endoscopic gastrostomy (PEG) for pharyngoesophageal malignancies are subject to develop malignant seeding at the PEG stoma. Risk factors include an untreated or recurrent advanced stage squamous cell carcinoma at the time of the PEG procedure and an endoscopic pull-string gastrostomy. Diagnosis is frequently late because this rare complication is poorly known. Prognosis remains poor despite aggressive treatment. Physicians should be aware of this complication in the event of hypermetabolic uptake around or close to a PEG stoma in patients monitored for a pharyngoesophageal squamous cell carcinoma.
Association between cardiovascular vs. non‐cardiovascular co‐morbidities and outcomes in heart failure with preserved ejection fraction
Authors: Lars H. Lund, Erwan Donal, Emmanuel Oger, Camilla Hage, Hans Persson, Ida Haugen‐Löfman, Pierre‐Vladimir Ennezat, Catherine Sportouch‐Dukhan, Elodie Drouet, Jean‐Claude Daubert, Cecilia Linde
Journal: European Journal of Heart Failure (2014)