Persistence of Hepatitis B Virus DNA Demonstrated by Polymerase Chain Reaction in Serum and Liver after Loss of HBsAg Induced by Antiviral Therapy
Authors: Patrick Marcellin
Journal: Annals of Internal Medicine (1990)

Brief Reports1 February 1990Persistence of Hepatitis B Virus DNA Demonstrated by Polymerase Chain Reaction in Serum and Liver after Loss of HBsAg Induced by Antiviral TherapyPatrick Marcellin, MD, Michèle Martinot-Peignoux, BS, Marie-Anne Loriot, PhD, Emile Giostra, MD, Nathalie Boyer, MD, Valérie Thiers, BS, Jean-Pierre Benhamou, MDPatrick Marcellin, MD, Michèle Martinot-Peignoux, BS, Marie-Anne Loriot, PhD, Emile Giostra, MD, Nathalie Boyer, MD, Valérie Thiers, BS, Jean-Pierre Benhamou, MDAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-112-3-227 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe polymerase chain reaction is a method of amplification of nucleic acids (1) that allows the detection of a very small amount of hepatitis B virus DNA (HBV-DNA), not detected by usual slot-blot or dot-blot hybridization (2-4). The presence of HBV-DNA has been previously shown using the polymerase chain reaction in the serum of patients with chronic hepatitis in the absence of serum hepatitis B surface antigen (HBsAg) (3, 4). We looked for HBV-DNA using the polymerase chain reaction in the serum, the mononuclear blood cells, and liver of two patients with chronic hepatitis B who seroconverted from HBsAg to...References1. SaikiGelfandStoffel RDS. Primer-directed enzymatic amplification of DNA with thermostable DNA polymerase. Science. 1988;239:487-91. CrossrefMedlineGoogle Scholar2. LarzulGuigueSninskyMackBrechotGuesdon DFJDCJ. Detection of hepatitis B virus sequences in serum by using in vitro enzymatic amplification. J Virol Methods. 1988;20:227-37. CrossrefMedlineGoogle Scholar3. ThiersNakajimaKremsdorf VED. Transmission of hepatitis B from hepatitis-B-seronegative subjects. Lancet. 1988;2:1273-6. CrossrefMedlineGoogle Scholar4. KanekoMillerFeinstone SRS. Detection of serum hepatitis B virus DNA in patients with chronic hepatitis using the polymerase chain reaction assay. Proc Natl Acad Sci USA. 1989;86:312-6. CrossrefMedlineGoogle Scholar5. ScottoHadchouelHeryYvartTiollaisBrechot JMCJPC. Detection of hepatitis B virus DNA in serum by a simple spot hybridization technique: comparison with the results of other viral markers. Hepatology. 1982;3:279-84. CrossrefGoogle Scholar6. PasquinelliLaureChatenoud CFL. Hepatitis B virus DNA in mononuclear blood cells. J Hepatol. 1986;3:95-103. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: From the Hôpital Beaujon, Clichy, France; and the Institut Pasteur, Paris, France. For current author addresses, see end of text. 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Potential for therapeutic interventionHepatic Histologic and Immunohistochemical Changes in Chronic Hepatitis B after Prolonged Clearance of Hepatitis B e Antigen and Hepatitis B Surface AntigenRobert P. Perrillo, MD, Elizabeth M. Brunt, MDIncidence of hepatitis B viraemia, detected using the polymerase chain reaction, after successful therapy of hepatitis B virus carriers with interferon–αRedevelopment of Hepatitis B Surface Antigen After Renal TransplantationThe hepatitis B virusAcute viral hepatitis 1 February 1990Volume 112, Issue 3Page: 227-228KeywordsAntiviral therapyBlood cellsChronic hepatitisChronic hepatitis BDNAHepatitis B surface antigenHepatitis B virusLiverNucleic acidsPolymerase chain reaction Issue Published: 1 February 1990 PDF DownloadLoading ...