tbuckley/cabot-search huggingface dataset:
Reduced Connexin43 Expression Inhibits Atherosclerotic Lesion Formation in Low-Density Lipoprotein Receptor–Deficient Mice
Authors: Brenda R. Kwak, Niels Veillard, Graziano Pelli, Flore Mulhaupt, Richard W. James, Marc Chanson, François Mach
Journal: Circulation (2003)

Gap junctions allow the direct exchange of ions and small molecules between cells in contact, thus coordinating physiological processes such as cell growth and differentiation. We have recently demonstrated increased expression of the gap junction protein connexin43 (Cx43) in specific subsets of cells in atherosclerotic lesions. Because the development of atherosclerosis depends critically on paracrine cell-to-cell interactions, we hypothesized that direct intercellular communication via gap junctions may be another factor controlling atherogenesis.The role of Cx43 in atherogenesis was examined by use of both a genetic and a pharmacological approach. First, atherosclerosis-susceptible LDL receptor-deficient (LDLR-/-) mice with normal (Cx43+/+) or reduced (Cx43+/-) levels of Cx43 were fed a cholesterol-rich diet for 14 weeks. The progression of atherosclerosis was reduced by 50% (P<0.01) in the thoracoabdominal aorta and in the aortic roots of Cx43+/-LDLR-/- mice compared with Cx43+/+LDLR-/- controls. Atheroma in Cx43+/-LDLR-/- mice contained fewer inflammatory cells and exhibited thicker fibrous caps with more collagen and smooth muscle cells. Next, we observed that HMG-CoA reductase inhibitors, or "statins," lipid-lowering drugs well known for their pleiotropic antiatherogenic effects, reduced Cx43 expression in primary human vascular cells in vitro. Atheroma of LDLR-/- mice treated orally with pravastatin contained fewer inflammatory cells and exhibited thicker fibrous caps than controls. This was associated with reduced Cx43 expression in lesions of statin-treated mice.These data indicate a critical role for Cx43-mediated gap junctional communication in atherosclerotic plaque formation.
The fractional disappearance rate of the separate isoenzymes of creatine phosphokinase in the dog
Authors: E. RAPAPORT, E. L. Dugan, B. Emilson
Journal: Cardiovascular Research (1975)

Separation of creatine phosphokinase (CPK) isoenzymes from canine tissue was accomplished successfully with column chromatography. Intravenous injection of each isoenzyme into six dogs resulted in a mono-exponential loss with differing fractional disappearance rates which averaged 0.65%, 0.86%, and 1.68%/min for the MM, MB, and BB isoenzymes, respectively. The distribution volume also varied: MM averaged 6.7%/body weight, MB 7.8%, and BB 10.4%.
Douglas Neil Sinclair
Authors: E C B Keat
Journal: BMJ (2005)

Concentration camp survivor and psychiatrist who became a high profile campaigner on child abuseThe Dutch psychiatrist and psychotherapy professor Dries van Dantzig once confessed to a colleague that he survived Neuengamme concentration camp "by making myself invisible"-which makes his survival seem all the more remarkable, as later he was rarely out of the public arena as a tireless and hard hitting promoter of mental health care.Imprisonment followed by years in a tuberculosis sanatorium may have sharpened the sense of injustice that reportedly drove Van Dantzig in his later public life.Today he is remembered as a merciless debater, publicist, political lobbyist, and film critic.His refusal to take "no for an answer" ensured that mental health and especially child abuse had a higher place on the political agenda of postwar Holland than it otherwise might have had.Van Dantzig was renowned for glaring from the front row at conference speakers with whom he disagreed.He made countless television appearances, and seemed to know a remarkable number of government ministers personally.The simple theme that underpinned his career was that, compared with somatic illness, psychiatric illness was largely ignored and underfunded.He illustrated this by arguing that every year more children would be killed by abuse than would ever die from Creutzfeldt-Jakob disease, which attracted millions more in funding.He suggested that up to 30% of the population had a neurosis, and that cases of child abuse could result in 50 to 80 deaths a year.Dries van Dantzig was born in 1920 into a progressive and left leaning family in Gouda.His education in Amsterdam was disrupted as the second world war broke out while he was still in his teens.He was eventually arrested for his involvement with the resistance and sent to Neuengamme near Hamburg.Of the 5500 Dutchmen sent there only 600 survived.Van Dantzig was among the few who did because, he later claimed, he was able to blend into the background amid such horrors as collecting dead bodies to take to the ovens or eating the food of those who had died.Yet all the time he was sharpening both his observational skills and his acute sense of injustice.
Application of Ultrafast Papanicolaou Stain in EUS-FNA Cytology
Authors: Cheng Tsu-Yao, Sun Meng-Shun, Yang Chang-Shyue, Chen Jiann-Hwa, Wang Hsiu-Po
Journal: Endoscopy (2011)

On-site cytology evaluation of EUS-FNA specimens is useful for quality assurance with improved diagnostic yield. Currently, the method for rapid assessment of FNA smears would be rapid Romanowsky-type stain such as Diff-Quik, Hemacolor stain or its equivalent. However, Romanowsky-type stain is inferior to Papanicolaou stain in subtle nuclear presentations. The objective of this study was to evaluate whether Ultrafast Papanicolaou (UFP) stain with 90-second protocol could be helpful in EUS-FNA on-site cytology.
Plastic Bags and Permanent Paper: <i>Annals</i> and Its Environments
Authors: Kathleen Case
Journal: Annals of Internal Medicine (1992)

Editorials1 August 1992Plastic Bags and Permanent Paper: Annals and Its EnvironmentsKathleen Case, MS Executive EditorKathleen Case, MS Executive EditorSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-117-3-261 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptYou may have noticed something missing from your Annals—the bag in which it is mailed. Starting with this issue, Annals will no longer come in a mailing bag or envelope; the address label will be put directly on the journal. This change will save approximately 1.6 million plastic bags per year. (Issues mailed with ACP Journal Club, and our international editions, will continue to be in bags.)Because the bags do protectAnnals, we first considered alternatives to eliminating them. Most of the so-called biodegradable plastics do not, in fact, degrade unless exposed to light, air, and water—conditions not guaranteed... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: Kathleen Case, MS Executive EditorAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 August 1992Volume 117, Issue 3Page: 261-261 ePublished: 1 December 2008 Issue Published: 1 August 1992 Copyright & Permissions© 1992 American College of PhysiciansPDF downloadLoading ...
Adrenal Ablation by Venous Catheter
Authors: Richard L. Eddy, James F. Mcmurry, Edward B. Best, Billy W. Henderson, V. Taylor Smith
Journal: Annals of Internal Medicine (1973)

Letters1 August 1973Adrenal Ablation by Venous CatheterRICHARD L. EDDY, M.D., F.A.C.P., JAMES F. MCMURRY JR., M.D., EDWARD B. BEST, M.D., BILLY W. HENDERSON, M.D., V. TAYLOR SMITH, M.D.RICHARD L. EDDY, M.D., F.A.C.P.Search for more papers by this author, JAMES F. MCMURRY JR., M.D.Search for more papers by this author, EDWARD B. BEST, M.D.Search for more papers by this author, BILLY W. HENDERSON, M.D.Search for more papers by this author, V. TAYLOR SMITH, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-79-2-273 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: When adrenalectomy is indicated for palliation of certain malignancies and for some patients with adrenocortical hyperfunction because of their poor surgical risk, a less threatening nonsurgical approach would be desirable. We report the successful ablation of adrenal function by instillation of a sclerosing substance through an angiographic catheter. The patient had Cushing's syndrome, caused by ectopic production of adrenocorticotrophin (ACTH) in metastatic carcinoma of the prostate.A 58-year-old man with stable diabetes mellitus of 10 years' duration and prostatic carcinoma (treated with orchiectomy and diethylstilbestrol) was admitted to the Endocrine Study Unit on 24 July 1972, with...References1. BAYLISSEDWARDSSTARER ROF: Complications of adrenal venography. Br J Radiol 43:531-533, 1970 CrossrefMedlineGoogle Scholar2. EAGANPAGE RM: Adrenal insufficiency following bilateral adrenal venography. JAMA 215:115-116, 1971 CrossrefMedlineGoogle Scholar3. FISHERTURNERHORTON CFR: Remission of primary hyperaldosteronism after adrenal venography. N Engl J Med 285:334-336, 1971 CrossrefMedlineGoogle Scholar4. FELLERMANDALAKOSSTREETEN HTD: Remission of Cushing's syndrome after unilateral adrenal phlebography. Ann Intern Med 73:585-589, 1970 LinkGoogle Scholar5. LECKYPLOTKIN JD: Adrenal function ablation utilizing the angiographic catheter. JAMA 218:1438, 1971 CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByUse of adrenal arterial embolization in severe ACTH-dependent Cushing's syndrome.Therapeutic adrenal venous infarction in ACTH-dependent Cushing's syndromeEctopic ACTH production in disseminated prostatic adenocarcinoma 1 August 1973Volume 79, Issue 2Page: 273-274KeywordsAdrenalectomyAdrenocorticotropic hormoneCathetersOrchiectomyType 2 diabetes Issue Published: 1 August 1973 PDF DownloadLoading ...
An outbreak of leptospirosis in cattle and man.
Authors: R J Hart, J Gallagher, S Waitkins
Journal: BMJ (1984)

On 20 May 1983 a 23 year old dairy farmer became ill with head? ache and aching joints. His symptoms worsened during the next week, and he was admitted to hospital on 27 May because he was vomiting and had neck stiffness. He was thought to have viral meningitis; a lumbar puncture produced cerebrospinal fluid containing 300 lymphocytes and 30 polymorphs per ul; protein and glucose were within normal limits. He was much better the next morning and was discharged on 29 May. He has remained well. His brother aged 20 took over the milking, and within the first few days he noted that five cows had an atypical mastitis, with a sudden drop in milk yield and clots in two or more quarters of the udder. There was, however, no pain or swelling of the udder. On 8 June a cow aborted. The fetus was submitted to Starcross Veterinary Investigation Centre, where infection with Leptospira interrogans serogroup Hebdomadis serotype hardjo was con? firmed by fluorescence antibody test in fetal lung and kidney, with the help of the Central Veterinary Laboratory, Weybridge. Subsequently the cow was found to have an antibody titre to L hardjo on the microscopic agglutination test of 1/1600 at abortion declining to 1/400 after two weeks, confirming recent infection. The farmer's mother read in the farming press a description of the human disease caused by L hardjo which closely matched her son's illness and communicated her sus? picions to their general practitioner. A specimen of her son's blood was collected on 5 July and compared with the specimen taken on 25 May. Rising titres from <1/10 to 1/40 to the lepto? spira group antigen were shown by complement fixation test. The Leptospira Reference Unit reported titres rising from < 1/40 to 1/1280 to serogroup Hebdomadis, serovar hardjo predominat? ing, in the microscopic agglutination test. On 1 July the farmer's brother developed an influenza-like illness. He was treated at home by the general practitioner with oral phenoxymethylpenicillin 500 mg four times daily, and his symptoms improved at first, but on 6 July he was admitted to hospital with headache, vomiting, and photophobia. No abnor? mal physical signs were observed, and his cerebrospinal fluid contained 240 red cells, three polymorphs, and three lympho? cytes per [?. He was treated with intravenous penicillin for five days followed by oral phenoxymethylpenicillin (500 mg four times daily) for a week. He was discharged from hospital on 12 July still suffering from mild frontal headaches. Sera collected on 2 and 12 July showed a rising complement fixation titre from <1/10 to 1/160 to leptospira. Microagglutination tests at the Leptospira Reference Unit indicated infection by L hardjo, with antibody titres rising from < 1/40 to 1/1280. He has since made a complete recovery and has remained well. Both patients have been confirmed serologically as suffering from L hardjo infection. Another cow aborted on 5 July, and infection with L hardjo was again confirmed by fluorescence antibody test.
851. Development of Organ Homing AAV-2 Vectors
Authors: Lorraine M. Work, Stuart A. Nicklin, Nicola Britton, Michael Hallek, Hildegard Buning, Andrew H. Baker
Journal: Molecular Therapy (2005)

Vascular endothelial heterogeneity in vivo has been defined using phage display in normal animals and in disease models. We performed in vivo phage biopanning to isolate organ targeting peptides in Wistar Kyoto (WKY) rats. A C7C-mer phage library was infused intravenously and allowed to circulate for 5 mins before saline perfusion and organ harvesting. Phage were extracted, amplified and re-infused over 2 subsequent rounds. Positive enrichment for phage pools homing to target organs was demonstrated (e.g. 130 and 1700-fold increases in lung homing phage between rounds 1-2 and 2-3). Sequencing phage clones for each target organ (brain, heart, lung, kidney; >130/organ) identified between 9 and 38 consensus peptides sequences for each. Subsequent studies using homogenous phage populations defined the targeting capacity of each peptide. Four candidate peptides [2 of which were found only in the lung (termed V and H), one from the brain (P) and one in all target organs but predominantly the lung and brain (Q)] were incorporated into the capsid of AAV-2 after position 587. Heparin sensitivity assays determined that 2 of the 4 modified vectors (Q- and V-modified AAVs) showed markedly diminished heparin sensitivity profiles compared to control. Organ biodistribution after systemic administration of these peptide-modified AAVs was investigated in WKY rats 1 hr and 28 days post virus infusion. The 2 heparin-independent AAV vectors showed altered 1 hr biodistribution profiles (determined by real-time PCR) vs. control. For example, Q-modified AAV showed a 6-fold and 2.5-fold increase in viral DNA levels in the lung and brain respectively, with a concomitant reduction in the liver level. In contrast, the V-modified AAV showed an 11-fold increase in viral DNA levels in the lung, however, levels present in the liver also increased. Neither the P- nor H-modified vectors showed altered biodistribution profiles compared to AAV CTL. Animals sacrificed 28 days post-viral infusion also demonstrated re-targeting for the 2 heparin-independent vectors using real-time PCR, with Q-modified AAV showing approximately 19- and 90-fold increases in viral DNA levels in the lung and brain respectively compared to control. Animals receiving V-modified AAV had an approximate 2.5 fold-increase in viral DNA in the lung. Immunohistochemical staining for |[beta]|-galactosidase detected transgene expression in cells lining the lumenal surface of blood vessels in the lung in the V-treated animals, no such positive staining was seen with either Q-modified or control AAV treated animals. Targeting peptides identified using in vivo biopanning drive organ-selective targeting of AAV-2 and may have potential for the development of novel targeted therapies for diverse diseases.
College Student Emergencies.
Authors: Dana L. Farnsworth
Journal: Annals of Internal Medicine (1967)

s1 May 1967College Student Emergencies.Dana L. Farnsworth, M.D.Dana L. Farnsworth, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-66-5-1066_3 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe role of a college psychiatric service in dealing with emergencies is described, since it can be a prototype of how general hospitals with psychiatric units may function.Crisis intervention is discussed. Situations precipitating crises include "acting out" behavior, anti-social acts by the emotionally disturbed, unwanted pregnancy, hallucinogenic drug ingestion, homosexual panic, or serious rupture in family relations. In a crisis it may not be clear who is the patient or whether anyone can be assigned that role.The most serious of all psychiatric emergencies among college students are those connected with suicidal attempts. Although exact data are not available... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: Dana L. Farnsworth, M.D.Affiliations: Cambridge, Massachusetts PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 May 1967Volume 66, Issue 5Page: 1066-1066KeywordsBehaviorDrugsEmotionsHomosexualsIngestionPregnancy ePublished: 1 December 2008 Issue Published: 1 May 1967 PDF downloadLoading ...
The human cytokine TSLP triggers a cell-autonomous dendritic cell migration in confined environments
Authors: Maria-Isabel Fernandez, Mélina L. Heuzé, Carolina Martinez-Cingolani, Elisabetta Volpe, Marie-Helene Donnadieu, Matthieu Piel, Bernhard Homey, Ana-Maria Lennon-Duménil, Vassili Soumelis
Journal: Blood (2011)
Glycosylations in the Globular Head of the Hemagglutinin Protein Modulate the Virulence and Antigenic Properties of the H1N1 Influenza Viruses
Authors: Rafael A. Medina, Silke Stertz, Balaji Manicassamy, Petra Zimmermann, Xiangjie Sun, Randy A. Albrecht, Hanni Uusi-Kerttula, Osvaldo Zagordi, Robert B. Belshe, Sharon E. Frey, Terrence M. Tumpey, Adolfo García-Sastre
Journal: Science Translational Medicine (2013)

Hemagglutinin glycosylations modulate the virulence and antigenicity of H1N1 influenza viruses.
Intrapulmonary Administration of CCL21 Gene-Modified Dendritic Cells Reduces Tumor Burden in Spontaneous Murine Bronchoalveolar Cell Carcinoma
Authors: Seok-Chul Yang, Raj K. Batra, Sven Hillinger, Karen L. Reckamp, Robert M. Strieter, Steven M. Dubinett, Sherven Sharma
Journal: Cancer Research (2006)

Abstract The antitumor efficiency of dendritic cells transduced with an adenovirus vector expressing secondary lymphoid chemokine (CCL21) was evaluated in a murine model of spontaneous bronchoalveolar cell carcinoma. The transgenic mice (CC-10 TAg) express the SV40 large T antigen (TAg) under the Clara cell promoter, develop bilateral, multifocal, and pulmonary adenocarcinomas, and die at 4 months as a result of progressive pulmonary tumor burden. A single intratracheal administration of CCL21 gene-modified dendritic cells (DC-AdCCL21) led to a marked reduction in tumor burden with extensive mononuclear cell infiltration of the tumors. The reduction in tumor burden was accompanied by the enhanced elaboration of type 1 cytokines [IFN-γ, interleukin (IL)-12, and granulocyte macrophage colony-stimulating factor] and antiangiogenic chemokines (CXCL9 and CXCL10) but a concomitant decrease in the immunosuppressive molecules (IL-10, transforming growth factor-β, prostaglandin E2) in the tumor microenvironment. The DC-AdCCL21 therapy group revealed a significantly greater frequency of tumor-specific T cells releasing IFN-γ compared with the controls. Continuous therapy with weekly intranasal delivery of DC-AdCCL21 significantly prolonged median survival by &amp;gt;7 weeks in CC-10 TAg mice. Both innate natural killer and specific T-cell antitumor responses significantly increased following DC-AdCCL21 therapy. Significant reduction in tumor burden in a model in which tumors develop in an organ-specific manner provides a strong rationale for further evaluation of intrapulmonary-administered DC-AdCCL21 in regulation of tumor immunity and genetic immunotherapy for lung cancer.(Cancer Res 2006; 66(6): 3205-13)
Desmoplastic Neurotropic Melanoma in a Patient With Trigeminal Neuralgia
Authors: Gerald Moulin-Romsee, Myriam Benamor, Sylvia Neuenschwander
Journal: Clinical Nuclear Medicine (2008)

A 51-year-old man presented with a hard subcutaneous nodule adhering to the underlying bone structures of the left eyelid in 2004. Histopathology showed a desmoplastic neurotropic melanoma (DNM) with perineural invasion. Patient presented with a first recurrence in October 2005, which was treated by surgery. In August 2006 he presented with trigeminal neuralgia of the left face in the area innervated by the first and second branches of the trigeminal nerve. A PET scan clearly shows the tumoral hypermetabolism of the subcutaneous recurrence of the neurotropic melanoma with invasion of the second or maxillary branch that follows the nerve up to the trigeminal ganglion, which was detected despite the physiological high uptake in the temporal lobe. MRI confirms the invasion and a second PET and MRI 6 months later done to evaluate resectability showed progressive disease. DNM is a rare subtype of spindle cell melanoma. It corresponds to dermal proliferation of desmoplastic cells of neural differentiation. Unlike other melanomas, however, survival for DNM may be better compared with other forms of melanoma. This rare case report presents PET imaging involving cranial nerve invasion by this uncommon melanoma subtype.
Treatment of Morphine-Type Dependence
Authors: John N. Chappel
Journal: JAMA (1972)

<h3>To the Editor.—</h3> The AMA Council Report "Treatment of Morphine-Type Dependence by Withdrawal Methods" (219:1611, 1972) is an excellent treatment of the subject. No references are given, which leads to the impression that the report is intended to stand as a definitive position by the AMA at this time. Clinical experience with large numbers of heroin addicts in the Illinois Drug Abuse Program provides the basis for the following suggested alterations in the Council's report. Deep aches, described as "being in my bones," are a common symptom of the methadone withdrawal syndrome. In addition, insomnia, often of the depressive type with early morning awakening, may persist for months following withdrawal from methadone hydrochloride. Decreasing the daily dose of methadone by 50% when the level of 20 mg is reached is too drastic a reduction. Subjective distress may be severe and lead to a discharge against medical advice.