tbuckley/cabot-search huggingface dataset:
Vi capsular polysaccharide-protein conjugates for prevention of typhoid fever. Preparation, characterization, and immunogenicity in laboratory animals.
Authors: S C Szu, A L Stone, J D Robbins, R Schneerson, J B Robbins
Journal: The Journal of Experimental Medicine (1987)

The Vi has proven to be a protective antigen in two double masked, controlled clinical trials in areas with high rates of typhoid fever (approximately 1% per annum). In both studies the protective efficacy of the Vi was approximately 70%. Approximately 75% of subjects in these areas responded with a fourfold or greater rise of serum Vi antibodies. In contrast, the Vi elicited a fourfold or greater rise in 95-100% of young adults in France and the United States. Methods were devised, therefore, to synthesize Vi-protein conjugates in order to both enhance the antibody response and confer T-dependent properties to the Vi (and theoretically increase its protective action in populations at high risk for typhoid fever). We settled on a method that used the heterobifunctional crosslinking reagent, N-succinimidyl-3-(2-pyridyldithio)-propionate (SPDP), to bind thiol derivatives of the Vi to proteins. This synthetic scheme was reproducible, provided high yields of Vi-protein conjugates, and was applicable to several medically relevant proteins such as diphtheria and tetanus toxoids. The resultant conjugates were more immunogenic in mice and juvenile Rhesus monkeys than the Vi alone. In contrast to the T-independent properties of the Vi, conjugates of this polysaccharide with several medically relevant proteins induced booster responses in mice and in juvenile Rhesus monkeys. Clinical studies with Vi-protein conjugates are planned. This scheme is also applicable to synthesize protein conjugates with other polysaccharides that have carboxyl functions.
Allergy prevalence increase in latex-sensitized spina bifida patients
Authors: A.M. Romeira, G. Pires, A. Gaspar, N. Godinho, V. Loureiro, V. Matos, E. Calado, M. Morais-Almeida, J. Rosado-Pinto
Journal: Journal of Allergy and Clinical Immunology (2003)
Letters: Questions Patients Should Ask about Laparoscopic Cholecystectomy
Authors: Raphael P. Nenner
Journal: Annals of Internal Medicine (1994)

Corrections15 May 1994Correction: Questions Patients Should Ask about Laparoscopic CholecystectomyFREERaphael P. Nenner, MDRaphael P. Nenner, MDIsland Peer Review Organization; Lake Success, NY 11042Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-120-10-199405150-00024 SectionsAboutVisual AbstractPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:Two sentences in a recently published letter [1] conveyed meanings other than those intended. In the sentence, “Ransohoff and Gracie appropriately advise caution in interpreting the published series, given the small number of open cholecystectomies,” the word “open” should be replaced by “laparoscopic”.In the fifth paragraph, the Island Peer Review Organization hotline described was not uniquely established for inquiries from beneficiaries regarding laparoscopic cholecystectomy but for inquiries on various medical care issues, including laparoscopic cholecystectomy. Thus, the sentence “To assist patients in assessing the experience and competency of their potential laparoscopic surgeon, in 1992 we instituted a toll-free hotline on laparoscopic cholecystectomy for Medicare and Medicaid beneficiaries,” should read “To assist patients with medical care issues, in 1992 we instituted a toll-free hotline for Medicare and Medicaid beneficiaries”.REFERENCE1. Nenner RP, Imperato PJ, Will TO. Questions patients should ask about laparoscopic cholecystectomy (Letter). Ann Intern Med. 1994; 120:443. Google Scholar Comments0 CommentsSign In to Submit A Comment Author, Article, and Disclosure InformationAffiliations: Island Peer Review Organization; Lake Success, NY 11042 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 15 May 1994Volume 120, Issue 10Page: 894KeywordsCholecystectomyLaparoscopic cholecystectomyMedicarePatientsPeer reviewSurgeons Issue Published: 15 May 1994 CopyrightCopyright © 1994 by American College of Physicians. All Rights Reserved.PDF DownloadLoading ...
Long-term follow-up in diabetic Charcot feet with spontaneous onset.
Authors: J Fabrin, K Larsen, P E Holstein
Journal: Diabetes Care (2000)

To assess the long-term results after Charcot breakdown with spontaneous onset in diabetic feet.This study was retrospective. A total of 115 patients (140 feet), 107 with acute deformity and 8 with chronic Charcot deformity, were followed for a median of 48 months (range 6-114). The routine treatment for acute cases was a weight-off regimen with crutches and foot protection with therapeutic shoes until skin temperature had normalized followed by increased weightbearing and the use of bespoke shoes or modification of conventional shoes.The incidence of Charcot deformity was 0.3%/year in the diabetic population investigated. About half of the patients were active in their jobs. Major complications were encountered in 5 (4%) of the patients that required surgical intervention: arthrodesis for unstable malaligned ankles in 3 subjects (1 bilaterally) and major amputation in 2 subjects for unstable ankle and pressure sores. Minor complications were recorded in 43% of subjects: new attacks of Charcot breakdown in 41 patients (36%) and/or foot ulceration in 43 patients (37%) that required minor surgical procedures for 11 patients. All healed except in 2 patients: 1 patient died before the Charcot fractures had healed, and 1 patient died with an unhealed ulcer. No patient lost the ability to walk independently.Major surgical procedures in only 4% were particularly related to patients with Charcot deformities in the ankle. Minor complications were recorded in about half of the patients. Lifelong foot care is required for diabetic patients with Charcot feet.
Mutations in VPS33B, encoding a regulator of SNARE-dependent membrane fusion, cause arthrogryposis–renal dysfunction–cholestasis (ARC) syndrome
Authors: Paul Gissen, Colin A Johnson, Neil V Morgan, Janneke M Stapelbroek, Tim Forshew, Wendy N Cooper, Patrick J McKiernan, Leo W J Klomp, Andrew A M Morris, James E Wraith, Patricia McClean, Sally A Lynch, Richard J Thompson, Bryan Lo, Oliver W Quarrell, Maja Di Rocco, Richard C Trembath, Hanna Mandel, S Wali, Fiona E Karet, A S Knisely, Roderick H J Houwen, Deirdre A Kelly, Eamonn R Maher
Journal: Nature Genetics (2004)
Abstract 5009: Thymoquinone downregulates n-cadherin, twist and snail expression and inhibits migration and invasion in cancer cells
Authors: Md. Asaduzzaman Khan, Manman Yang, Chunli Wei, Lin Gan, Junjiang Fu
Journal: Cancer Research (2014)

Abstract Background: Metastasis is the major characteristic of malignant carcinomas, which has been found to be linked with epithelial to mesenchymal transitions (EMT). Regulation of EMT process can provide a significant beneficiary effect to control metastasis, as well as to treat cancers. In this study we have investigated the effects of natural product Thymoquinone in metastatic cancer cell lines to evaluate their role in metastatic characteristics linked to EMT process. Methods: We have treated HeLa and MDA-MB-435 human cancer cell lines with Thymoquinone, and analyzed the cell proliferation, migration and invasion, which are the major metastatic characteristics, by using the real-time cell analyzer. The real-time PCR and Western blot analysis was employed for the determination of the cellular expression of major EMT-associated proteins N-cadherin and E-cadherin; their transcription factors Twist, Snail, Slug and Zeb; and another important metastatic protein Vimentin. Results: Thymoquinone was found to inhibit the migratory and invasive characteristics in both cancer cell lines dose dependently, and effective in a low dose eventually (1-5 μM). Its cytotoxicity has also been confirmed by MTT assay for cell viability. Genetic expression analysis showed that Thymoquinone significantly down-regulates the mRNA expression of cell adhesion protein N-cadherin in HeLa cell. Thymoquinone also down-regulates the transcription factor Twist, which is being considered as an oncoprotein. Thymoquinone has mild to moderate effect on the expression pattern of other metastatic proteins in HeLa cells. In MDA-MB-435, Thymoquinone treatment downregulates Twist, Snail, Vimentin and N-cadherin. However, N-cadherin expression was found in extremely low in both untreated and treated MDA-MB-435. Conclusions: We report that the prospective anticancer molecule Thymoquinone down-regulates N-cadherin and its transcription factors like Twist and Snail, which might have a significant influence on the effects of Thymoquinone in controlling cell migration and invasion. Further research is necessary for the investigation of this compound in specific cancer types with mechanisms in detail. Fund support: This work was supported in part by the National Natural Science Foundation of China (81172049, 30371493), Science and Technology Innovation Team of Colleges and Universities of Sichuan Province (13TD0032). Citation Format: Md. Asaduzzaman Khan, Manman Yang, Chunli Wei, Lin Gan, Junjiang Fu. Thymoquinone downregulates n-cadherin, twist and snail expression and inhibits migration and invasion in cancer cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5009. doi:10.1158/1538-7445.AM2014-5009
Effects of trifluoperazine on function and structure of toad urinary bladder. Role of calmodulin vasopressin-stimulation of water permeability.
Authors: S D Levine, W A Kachadorian, D N Levin, D Schlondorff
Journal: Journal of Clinical Investigation (1981)

Calcium ion plays a major regulatory role in many hormone-stimulated systems. To determine the site of calcium's action in the toad urinary bladder, we examined the effect of trifluoperazine, a compound that binds specifically to the calcium binding protein, calmodulin, and thereby prevents activation of enzymes by the calcium- calmodulin complex. 10 microM trifluoperazine inhibited vasopressin stimulation of water flow, but did not alter vasopressin's effects on urea permeability or short-circuit current. Trifluoperazine also blocked stimulation of water flow by cyclic AMP and methylisobutylxanthine, implying a "postcyclic AMP" site of action. Consistent with these results, trifluoperazine did not decrease epithelial cyclic AMP content or the cyclic AMP-dependent protein kinase activity ratio. Assay of bladder epithelial supernate demonstrated calmodulin-like activity of 1.5 U/microgram protein. Morphologic studies of vasopressin-treated bladders revealed that trifluoperazine did not alter the volume density of cytoplasmic microtubules or significantly decrease the number of fusions between cytoplasmic, aggregate-containing, elongated vesicles and the luminal membrane. Nonetheless, the frequency of luminal membrane aggregates, structures that correlate well with luminal membrane water permeability, was decreased by greater than 50%. Thus, trifluoperazine appears to inhibit the movement of intramembranous particle aggregates from the fused intracellular membranes to the luminal membrane, perhaps by blocking an effect of calcium on microfilament function.
A CLINICAL STUDY IN PELVIC MASSAGE.
Authors: HOMER C. BLOOM
Journal: JAMA (1898)

The purpose and scope of this paper is not to discuss the merits or demerits of pelvic massage. This is a matter which will necessarily follow the adoption of any new treatment, and especially one that is in any way open to objection. At the very threshold of this subject it is easy to anticipate the objections which are likely to be raised relative to the handling of organs and tissues which are the local seat of sexuality and passion. Yet that liberality which characterizes the true physician and surgeon will always seek to find the level by which any new treatment can be taken. Therefore, it is not for any one to emphasize or declare the status of a treatment which must be judged entirely upon those results which accrue from its practical application. Accordingly, this paper will not only deal with the positive but with the negative results,
Increased Sensitivity to N-Methyl-D-Aspartate Receptor-Mediated Excitotoxicity in a Mouse Model of Huntington's Disease
Authors: Melinda M Zeron, Oskar Hansson, Nansheng Chen, Cheryl L Wellington, Blair R Leavitt, Patrik Brundin, Michael R Hayden, Lynn A Raymond
Journal: Neuron (2002)
An in vivo animal model of gene therapy for leukocyte adhesion deficiency.
Authors: J C Krauss, L A Mayo-Bond, C E Rogers, K L Weber, R F Todd, J M Wilson
Journal: Journal of Clinical Investigation (1991)

Leukocyte adhesion deficiency (LAD) is an inherited disorder of leukocyte function that is caused by defects in the CD18 gene and is associated with diminished cell surface expression of CD11/CD18 proteins. We have developed an in vivo model for gene therapy of LAD. Recombinant retroviruses were used to transduce a functional human CD18 gene into murine bone marrow cells which were transplanted into lethally irradiated syngeneic recipients. A reliable flow cytometric assay for human CD18 in transplant recipients was developed based on: (a) the availability of human specific CD18 monoclonal antibodies and (b) the observation that human CD18 can form chimeric heterodimers with murine CD11a on the cell surface. Human CD18 was detected on leukocytes in a substantial number of transplant recipients for at least 6 mo suggesting that the gene had been transduced into stem cells. Expression was demonstrated in several lineages of a variety of hematopoietic tissues, but was consistently highest and most frequent in granulocytes. Murine granulocytes demonstrated appropriate posttranscriptional regulation of human CD18 in response to activation of protein kinase C. No apparent untoward effects of human CD18 expression were noted in transplant recipients. These studies suggest a specific strategy for LAD gene therapy that may be effective and safe.
P558 IMPACT OF SCREENING ON SURVIVAL OF HEPATOCELLULAR CARCINOMA (HCC) IN HIV/HBV-COINFECTED PATIENTS
Authors: A. Aytaman, R.K. Fox, M. Núñez, M. Jain, E. Vispo, L. Kikuchi, E. Page, L. Taylor, B. Mínguez, M. Ventura, N. Merchante, D.E. Kaplan, M. Harris, H. Klinker, M.D. Hernández, K.K. Hunt, J.A. Pineda, M. Nelson, P. Barreiro, N. Bräu
Journal: Journal of Hepatology (2014)
Left ventricular hypertrophy induces an increase in N-terminal pro-brain natriuretic peptide levels in subjects with and without hypertension: A population study
Authors: Miguel Rivera, Fernando Garcia de Burgos, Francisco Sogorb, Vicente Miro, Vicente Betomeu, Antonio Salvador, Rafael Paya, Juan Cosin, Guillermo Grau, Alejandro Jordan, Vicente Climent, Jose Luis Bosca, Jose Luis Diago, Araceli Frutos, Luis Mainar, Vicente Mora, Alicia Ibañez, Juan Moreno, Jose Valencia, Roberto Elosua, Iidelfonso Roldan, Ricardo Gomez
Journal: Journal of the American College of Cardiology (2003)
C7 is expressed on endothelial cells as a trap for the assembling terminal complement complex and may exert anti-inflammatory function
Authors: Fleur Bossi, Lucia Rizzi, Roberta Bulla, Alessandra Debeus, Claudio Tripodo, Paola Picotti, Elena Betto, Paolo Macor, Carlo Pucillo, Reinhard Würzner, Francesco Tedesco
Journal: Blood (2009)
Molecular epidemiology in cancer risk assessment and prevention: recent progress and avenues for future research.
Authors: G N Wogan
Journal: Environmental Health Perspectives (1992)

Molecular epidemiology is increasingly being applied in studies of cancer risks derived from exposure to environmental carcinogens of both endogenous and exogenous origins. Analytical methods have been developed that are capable of detecting and quantifying levels of covalent adducts of several important classes of carcinogens with cellular DNA and blood proteins. Methods of sufficient sensitivity and specificity to detect ambient levels of exposure are in current use. These are being used in studies related to tobacco use (polycyclic aromatic hydrocarbons, aromatic amines, tobacco-specific nitrosamines); dietary exposures (aflatoxins, N-nitrosamines, heterocyclic amines); medicinal exposures (cisplatin, alkylating agents, 8-methoxypsoralen, ultraviolet photoproducts); occupational exposures (aromatic amines, polycyclic aromatic hydrocarbons, oxides of ethylene and styrene, and vinyl chloride); and oxidative damage (8-hydroxyguanine, thymine glycol). Methodologic improvements together with their expanded use in feasibility studies continue to produce results that support the validity of this approach for detecting and quantifying exposure to carcinogens. Genetic markers are also being used to detect early biological responses in efforts to link carcinogen exposure to initiating events in the carcinogenesis process. These include, in addition to traditional cytogenetic markers (e.g., chromosomal aberrations, sister chromatid exchange, micronuclei), other alterations in chromosomal structure such as restriction fragment length polymorphisms, loss of heterozygosity, and translocation markers. Specific genetic changes have recently been identified as critical molecular events in the initiation and development of many cancers. Important among these are activation of oncogenes, especially those of the ras family, and inactivation of tumor-suppressor genes (e.g., p53 and Rb) by point mutations and/or chromosomal deletions and other structural changes. Although some of these changes are known to occur in chemically induced tumors of experimental animals, the possible role of chemical carcinogens in the induction of genetic abnormalities in human cancers has yet to be determined. Continuing investigations employing the methods of molecular epidemiology promise to provide further evidence concerning these relationships. Future investigations employing newly developed molecular biological methods, in particular those based on polymerase chain reaction amplification of DNA, to identify alterations in DNA and chromosomal structure, combined with methods for characterizing exposure to carcinogens and early effects, have great potential for further elucidating the role of genotoxic agents in the etiology of human cancers and also for the development of strategies for their prevention.
Massive Hemobilia From Ruptured Hepatic Artery Aneurysm
Authors: John H. Hughes
Journal: JAMA (1970)

<h3>To the Editor.—</h3> Massive upper gastrointestinal tract bleeding can be a difficult problem, but recurrent massive hemobilia can be even more challenging. Such a case diagnosed in a small community hospital as a rupture of an hepatic artery aneurysm into the common bile duct was recently successfully treated. In addition, it is an example of what one can achieve by cooperative regional medical planning. <h3>Report of a Case.—</h3> This 67-year-old white woman was admitted to Hardin Memorial Hospital on Jan 24, 1970, for pain in the right upper quadrant of the abdomen. Two years previously she had had similar pains and no abnormality had been seen on cholecystograms. She was afebrile on admission with a pulse rate of 80 beats per minute and blood pressure of 150/90 mm Hg. She was nauseated but tolerated a low fat diet for a day with a fever of 101 F appearing 12 hours
Total Exchangeable Sodium in Depressive Illness
Authors: A. Coppen, D. M. Shaw, A. Mangoni
Journal: BMJ (1962)

intake in 24 hours was 900 calories, and the majority of intakes were much lower than this.The mean daily rise of serum urea in this group remained unchanged throughout the duration of their illness, contrasting with the reductions obtained in the 12 patients studied while receiving the prepared diets.It is possible that the improvement could be due merely to the provision of a higher intake of calories, but, nevertheless, it is apparent that the inclusion of some protein into the diet had no adverse effect, nor did it increase overall nitrogen catabolism.We believe that provision of exogenous protein may well be a vital factor in the survival of those cases of acute renal failure of surgical aetiology, and of those cases in which repeated haemodialysis may be required before recovery of renal function occurs.Appendix Protein Sodium Potassium Calories _. mg.mg.i pt.(300 ml.) milk daily.. 9 0 144-0 460 0 190 Breakfast: i oz.(7.5 g.) oatmeal .. 085 24 26-0 28 1,, (30,, ) lactose .. 112 1,, (30,,) cream .. 04 7-6 22-0 130 100 ml.water Dinner: 1 oz.(30 g.) white fish .. 51 340 790 26 j.,, (5 ,,)butter .. 30 0 2-0 112 , (15, )potatoes .. 0-25 6-0 47-0 10 1 (30,,) vegetables .. 6-4 31-0 6 Tea: 1 oz.(30 g.) bread .. 22 147-0 30-0 69 i ,, (7 5,, ) butter ..
Molecular Basis of Giant Cells in Tuberous Sclerosis Complex
Authors: David J. Kwiatkowski, Brendan D. Manning
Journal: New England Journal of Medicine (2014)

The clinical observation of giant-cell brain tumors in a 15-year-old girl with epilepsy (the first patient noted to have tuberous sclerosis) has led to elucidation of the mTOR pathway, a critical regulator of cell size and growth.
Telomerase activation by hTRT in human normal fibroblasts and hepatocellular carcinomas
Authors: Jun-ichi Nakayama, Hidetoshi Tahara, Eiji Tahara, Motoki Saito, Kaori Ito, Hideo Nakamura, Toshio Nakanishi, Eiichi Nakanishi, Toshinori Ide, Fuyuki Ishikawa
Journal: Nature Genetics (1998)
Histocompatibility Antigens in Active Chronic Hepatitis and Primary Biliary Cirrhosis
Authors: R. M. Galbraith, A. L. W. F. Eddleston, M. G. M. Smith, R. Williams, R. N. M. McSween, G. Watkinson, H. Dick, L. A. Kennedy, J. R. Batchelor
Journal: BMJ (1974)

The frequency of antigens HL-A 1 (48%) and HL-A 8 (52%) in 54 patients with active chronic hepatitis from south-east England was significantly higher than in 89 control subjects from the same region (22% and 17% respectively). No correlation could be detected with the age and sex of the patients or with the presence of a particular immunological abnormality but the frequency of HL-A 1 and HL-A 8 was much lower in the nine patients who were positive for HBAg than in the 45 HBAg-negative cases. These results provide further evidence of the importance of genetic factors in active chronic hepatitis. In contrast the frequency of HL-A 1 and HL-A 8 in primary biliary cirrhosis, both in 45 patients from south-east England and in 28 patients from western Scotland, was not significantly different from that found in control groups from the same regions.
A phase I study of lenalidomide in combination with fludarabine and rituximab in previously untreated CLL/SLL
Authors: Jennifer R Brown, Jeremy Abramson, Ephraim Hochberg, Evgeny Mikler, Virginia Dalton, Lillian Werner, Hazel Reynolds, Christina Thompson, Sean M McDonough, Yanan Kuang, Jerome Ritz, Donna Neuberg, Arnold S Freedman
Journal: Leukemia (2010)