tbuckley/cabot-search huggingface dataset:
Abstract 3271: The RNA binding protein HuR controls angiogenesis in triple negative breast cancer
Authors: Ulus Atasoy, Matthew Gubin, Bob Calaluce, Wade Davis, Joseph Magee, Connie Strouse, Daniel Shaw, Timothy Hoffman, Tammy Rold
Journal: Cancer Research (2010)

Abstract Due to the poor correlation between steady state mRNA levels and protein products, traditional microarray analysis may miss many genes which are regulated primarily at the level of mRNA stability and translation. Posttranscriptional gene regulation mediated by microRNAs and RNA binding proteins (RBPs) is being recognized as an important form of gene regulation. The elav (embryonic lethal abnormal vision) family of RBPs, are paraneoplastic antigens, over-expressed in a variety of malignancies, including breast cancer. Antibodies against elav family members are believed to be cancer-protective. The elav family binds to the AU-rich elements (AREs) found in the 3’ untranslated regions (UTRs) of many early-response genes, including proto-oncogenes and cell cycle regulators. HuR, the ubiquitously expressed family member, has been described to play a role in cancer progression by stabilizing and translationally up regulating expression of its target mRNAs. Elevated levels of cytoplasmic HuR directly correlate with increased invasiveness of malignancy and poor prognosis for many cancers, including those of the breast. HuR has been described to positively control the expression of multiple genes in the acquired capabilities model, such as VEGF and HIF1α. Hence, it has been suggested that HuR may serve as a tumor maintenance gene which allows for cancers to proliferate. Therefore, it is of interest to discover in vivo HuR targets, as these genes may play vital roles in transformed cells. We have developed methods called RNA immunoprecipitations applied to microarrays, RIP-Chip. We used RIP-Chip to identify distinct subsets of HuR associated mRNAs in MDA-MB-231 and MCF-7 breast cancer cell lines and validated several novel targets. To further investigate the role of HuR in triple negative breast cancer, we over expressed HuR in MDA-MB-231 cells, which results in accelerated growth and alterations in cell cycle kinetics. Surprisingly, when employed in orthotopic mouse models of cancer, HuR over expression significantly inhibited growth of triple negative tumors by 90%. Putative mechanisms appear to be anti-angiogenic, as HuR over expression increases anti-angiogenic factors, but surprisingly, also down regulates pro-angiogenic factors such as VEGF. These results are highly significant because they implicate HuR as a master regulator of angiogenesis in triple negative breast cancer tumor formation and suggest potentially novel treatment methods for this aggressive form of breast cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3271.
Quantitative Studies in Man of the Cardiovascular Effects of Reflex Vagal Stimulation Produced by Carotid Sinus Pressure
Authors: ARTHUR J. LINENTHAL, LEE CAPLITZ
Journal: Circulation (1954)

In each of three individuals, carotid sinus pressure produced a shift of the control of the heart beat from the dominant pacemaker to an ectopic, slower or faster, site of impulse formation. In each instance, it was possible to measure the fundamental rates of the two practically simultaneous pacemakers over fairly wide ranges of variation in rate. A direct, linear relationship was found to exist.
Oxidized LDL Decreases <scp>l</scp> -Arginine Uptake and Nitric Oxide Synthase Protein Expression in Human Platelets
Authors: L.Y. Chen, P. Mehta, J.L. Mehta
Journal: Circulation (1996)

Oxidized LDL (ox-LDL) promotes vasoconstriction and platelet activation. The present study was undertaken to determine the involvement of the L-arginine-nitric oxide (NO) pathway in ox-LDL-mediated platelet activation.Washed human platelets were incubated with native LDL or ox-LDL for 1 hour at 37 degrees C followed by measurement of platelet function and indexes of the L-arginine-NO pathway. Ox-LDL but not native LDL caused a concentration-dependent increase in thrombin-induced platelet aggregation and 14C-serotonin release. These effects of ox-LDL were inhibited by pretreatment of platelets with L-arginine, the precursor of NO. Ox-LDL also caused a concentration-dependent reduction in the uptake of 3H-L-arginine by platelets. In addition, NO synthase activity, measured as conversion of 3H-L-arginine to 3H-L-citrulline, decreased on incubation of platelet cytosol with ox-LDL. Nitrite production was also reduced by treatment of platelets with ox-LDL. These effects of ox-LDL on NO synthase activity and nitrite production were reversed by pretreatment of platelets with L-arginine. Concurrent with the decrease in NO production, cytosolic cGMP was inhibited in ox-LDL-treated platelets. The inhibitory effects of ox-LDL were dependent in part on the increase of cholesterol in the platelets. Western blot analysis demonstrated approximately 50% reduction in the expression of NO synthase protein in platelets treated with ox-LDL.These observations indicate that the L-arginine-NO pathway is involved in the effects of ox-LDL on platelet function and that ox-LDL stimulates platelet function primarily by diminishing NO synthase expression as well as decreasing the uptake of L-arginine.
Picasso, Velasquez, and Dwarfs
Authors: James G. Ravin
Journal: JAMA (1974)

ARTISTS often turn to the works of others for themes and ideas. Pablo Picasso, the foremost artist of this century, did so to a great extent. He incorporated into his works varying themes from primitive African sculpture to the paintings of Cranach, Poussin, El Greco, Manet, Delacroix, and Toulouse-Lautrec. Picasso's most extensive use of another artist's theme is his series of 45 versions on Velasquez' "The Maids of Honor" (Fig 1). <h3>Velasquez</h3> Velasquez was court painter to King Philip IV of Spain and held exclusive rights to portrayal of the king. In addition, he acted as an ambassador for Spain. "The Maids of Honor" is considered by some critics to be the finest masterpiece of Spain's greatest painter. The painting is admired for its composition, which revolves around five-year-old Princess Margarita attended by her maids of honor. Critics have praised the work for its sensation of depth, the graceful portrayal
Only the CD62L+ subpopulation of CD4+CD25+ regulatory T cells protects from lethal acute GVHD
Authors: Joerg Ermann, Petra Hoffmann, Matthias Edinger, Suparna Dutt, Francis G. Blankenberg, John P. Higgins, Robert S. Negrin, C. Garrison Fathman, Samuel Strober
Journal: Blood (2004)
Cytophilic Antibodies in Man
Authors: MALCOLM S. MITCHELL
Journal: Annals of Internal Medicine (1973)

Antibodies cytophilic for macrophages, mediating attachment of immunologically naive macrophages to leukemic cells, were found in the serum of 25 patients with acute myelocytic or lymphocytic leukemia, regardless of their clinical status. These antibodies recognized not only the patient's own (autochthonous) tumor cells but also second-party (allogenic) cells of the same histological type. There was no cross-reactivity between the two types of leukemia except in one instance. Sorption with leukemic cells but not normal leukocytes removed the cytophilic antibody activity, and there was no reactivity against normal leukocytes derived from a patient in remission from lymphocytic leukemia. Thus, the cytophilic antibodies were directed against leukemia-associated antigens common to a histological class of leukemia. The presence of cytophilic antibodies can be shown in man, paralleling studies in mice showing the pivotal importance of this class of antibody in the rejection of tumors by macrophages.
OBSERVATIONS ON THE EFFECTS OF ANTI-HISTAMINE DRUGS ON THE REACTIONS OF THE SKIN VESSELS TO CARBAMINOYL CHOLINE
Authors: E. J. MOYNAHAN
Journal: British Journal of Dermatology (1952)

Journal Article OBSERVATIONS ON THE EFFECTS OF ANTI‐HISTAMINE DRUGS ON THE REACTIONS OF THE SKIN VESSELS TO CARBAMINOYL CHOLINE Get access E. J. MOYNAHAN E. J. MOYNAHAN Departments of Chemical Pathology and Dermatology, Guy's Hospital Medical School, London Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 64, Issue 1, 1 January 1952, Pages 1–9, https://doi.org/10.1111/j.1365-2133.1952.tb16155.x Published: 01 January 1952
ADDRESS ON STATE MEDICINE.
Authors: HENRY D. HOLTON
Journal: JAMA (1895)

<h3>Mr. President, Members of the American Medical Association:</h3> —Hygiene is as old as the profession of medicine. But "State Medicine" is of comparatively recent origin. We find the leader of Israel promulgating sanitary regulations for his people that can hardly be improved to-day. Greeks and Romans honored the sanitarian and gave much time to the study of subjects connected with matters pertaining to public health. Pure air, pure water were deemed by them to be a great desideratum. The Aztec erected aqueducts to supply the City of Mexico with pure water long before Columbus sailed from Palos. But amid the wreck of the Dark Ages the little that had been taught regarding the necessities of hygiene to public health was buried, and only in the last sixty years has its resurrection and reëstablishment upon a firm and more enduring basis been attempted. The early pioneers of our profession in this
Overexpression of Fibroblast Growth Factor-10 during Both Inflammatory and Fibrotic Phases Attenuates Bleomycin-induced Pulmonary Fibrosis in Mice
Authors: Varsha V. Gupte, Suresh K. Ramasamy, Raghava Reddy, Jooeun Lee, Paul H. Weinreb, Shelia M. Violette, Andreas Guenther, David Warburton, Barbara Driscoll, Parviz Minoo, Saverio Bellusci
Journal: American Journal of Respiratory and Critical Care Medicine (2009)

Fibroblast growth factor-10 (FGF10) controls survival, proliferation, and differentiation of distal-alveolar epithelial progenitor cells during lung development.To test for the protective and regenerative effect of Fgf10 overexpression in a bleomycin-induced mouse model of pulmonary inflammation and fibrosis.In SP-C-rtTA; tet(O)Fgf10 double-transgenic mice, lung fibrosis was induced in 2-month-old transgenic mice by subcutaneous delivery of bleomycin (BLM), using an osmotic minipump for 1 week. Exogenous Fgf10 expression in the alveolar epithelium was induced for 7 days with doxycycline during the first, second, and third weeks after bleomycin pump implantation, and lungs were examined at 28 days.Fgf10 overexpression during Week 1 (inflammatory phase) resulted in increased survival and attenuated lung fibrosis score and collagen deposition. In these Fgf10-overexpressing mice, an increase in regulatory T cells and a reduction in both transforming growth factor-beta(1) and matrix metalloproteinase-2 activity were observed in bronchoalveolar lavage fluids whereas the number of surfactant protein C (SP-C)-positive, alveolar epithelial type II cells (AEC2) was markedly elevated. Analysis of SP-C and TUNEL (terminal deoxynucleotidyltransferase dUTP nick end labeling) double-positive cells and isolation of AEC2 from lungs overexpressing Fgf10 demonstrated increased AEC2 survival. Expression of Fgf10 during Weeks 2 and 3 (fibrotic phase) showed significant attenuation of the lung fibrosis score and collagen deposition.In the bleomycin model of lung inflammation and fibrosis, Fgf10 overexpression during both the inflammatory and fibrotic phases results in a greatly reduced extent of lung fibrosis, suggesting that FGF10 may be useful as a novel approach to the treatment of pulmonary fibrosis.
Improvement in clinical work through feedback: intervention study
Authors: R. Jorde, A. Nordoy
Journal: BMJ (1999)

P = 0.04 and raised dust there (15.7, 2.4 to 651; P = 0.01.)Two variables were constructed to refine the logistic regression analysis.For forests the variable was defined by those who spent more than 16 hours a month in forests, who went to forests for wood, or who picked up wood or were exposed to dust or earth during a leisure visit.For exposure in buildings where they may have been rodents the variable was defined by those who spent more than 2 hours there and who cleaned, raised dust, or made a vigorous physical effort.In the final model of the conditional logistic regression analysis, cases were more likely to live less than 50 metres from a forest and have seen rodents in or around their home, to have been digging, to have spent long periods in forests and been in contact with wood or disturbed earth or dust (table).Rodent control was more common among controls.Cases were more likely than controls to both live near a forest and see rodents at home (66.1, 5.7 to 768.9).
T-Lymphocyte Subpopulations in Homosexual Men
Authors: Hardy Kornfeld, Robert A. Vande Stouwe, Michael Lange, M. Mohan Reddy, Michael H. Grieco
Journal: New England Journal of Medicine (1982)

IN the past two years there has been an unprecedented outbreak of Kaposi's sarcoma, Pneumocystis carinii pneumonia, and other opportunistic infections in male homosexuals and, to a lesser extent, in drug addicts in the United States.1 , 2 These cases have been concentrated in New York City and California, and as of January 13, 1982, 216 cases had been reported to the Centers for Disease Control.3 Nineteen cases with immunologic evaluation were reported in December 1981.4 5 6 All patients had evidence of cellular immunodeficiency with cutaneous anergy, reduced numbers of T lymphocytes, and impaired lymphocyte transformation. Four patients described by Gottlieb et al. . . .
Smiley's People
Authors: Beth Henneman
Journal: JAMA (1989)

To the Editor.—  Hospitals are stressful places, not only for patients and families, but for staff as well. There are many methods of  reduction available to the health care professional, but few are readily accessible. We suggest the use of a simple but poorly studied method of decreasing stress, the smile. Smiling is an unsophisticated yet effective means of social contact. Selye 1 noted that a lack of socialization plays a role in perpetuating the stress  Increased smiling between health care professionals could, therefore, play a role in decreasing stress. The following study was undertaken to determine the effectiveness of a smile in eliciting a smiling response.  A multicenter trial using a prospective, randomized design was undertaken to test the following hypothesis: medical personnel who are smiled at will smile more than those who are not smiled at, or, in medical terms, a smile is contagious. Three hundred
DECREASED PLASMA ADIPONECTINE LEVELS IN ASYMPTOMATIC DIABETIC PATIENTS WITH OBSTRUCTIVE CORONARY DISEASE
Authors: Roxana Djaberi, Joanne D. Schuijf, Cornelis J. Roos, Alberto M. Pereira, Jan W. Smit, Arthur J. Scholte, Johannes A. Romijn, Ernst E. van der Wall, Jeroen J. Bax, J. Wouter Jukema
Journal: Journal of the American College of Cardiology (2010)
Ophthalmic Clinics in Industry
Authors: M. G. Aird
Journal: BMJ (1966)

MEDICALJOURNAIThere are now enough facilities in India for postgraduate study in nearly every branch of medicine ; moreover, Indian postgraduate qualifications are essential for higher appoint- ments in our teaching hospitals.In the circumstance, the only legitimate study over- seas would be for acquiring special skills and not degrees or diplomas.This would entail the availability of clinical appoint- ments in specialist teaching hospitals, which is not the case in Britain from all available accounts, including Dr. Chakrabartty's.The restrictions on obtaining foreign exchange for overseas study are designed to make the best use of this scarce resource by ensuring that only those fitted for such study are able to proceed.A beneficial " side- effect" is that since the end of last year the medical influx into Britain has come to an end.-Iam, etc.,
Oral ulceration: a diagnostic problem.
Authors: C E Grattan, C Scully
Journal: BMJ (1986)

Oral ulceration: a diagnostic problem Most mouth ulcers are caused by trauma or are aphthous.Nevertheless, they may be a manifestation of a wide range of mucocutaneous or systemic disorders, including infections, drug reactions, and disorders of the blood and gastro- intestinal systems, or they may be caused by malignant disease.The term mouth ulcers should not, therefore, be used as a final diagnosis.
Magnetic resonance imaging in osteoarthritis of the knee: correlation with radiographic and scintigraphic findings.
Authors: T E McAlindon, I Watt, F McCrae, P Goddard, P A Dieppe
Journal: Annals of the Rheumatic Diseases (1991)

Twelve knees with a range of severity of knee osteoarthritis were assessed by magnetic resonance imaging (MRI) and technetium-99m labelled hydroxymethylene diphosphonate scintigraphy. Five magnetic resonance pulse sequences were evaluated. Proton density (TR = 1000, TE = 26 ms) and STIR (TR = 1500, TI = 100, TE = 30 ms) were chosen for further use. Abnormalities shown by MRI included joint effusions, meniscal disruption, hyaline cartilage thinning, subchondral signal changes, pseudocysts, and heterogeneity of signal from osteophytes. Certain MRI and scintigraphic appearances correlated: (a) 'hyperintense osteophytosis' and ipsilateral 'tramline' scintigraphic uptake, suggesting increased fat content in 'active' osteophytes; (b) subchondral signal change and 'extended' pattern, possibly reflecting inflammation, synovial leak, or fibrovascular repair; (c) patellofemoral joint signal changes and patellar isotope uptake.
Depressive Symptom Clusters and 5-Year Incidence of Coronary Artery Calcification
Authors: Jesse C. Stewart, Desiree J. Zielke, Misty A.W. Hawkins, David R. Williams, Mercedes R. Carnethon, Sarah S. Knox, Karen A. Matthews
Journal: Circulation (2012)

Because depression is a multidimensional construct and few studies have compared the relative importance of its facets in predicting cardiovascular risk, we evaluated the utility of depressive symptom clusters in predicting the 5-year incidence of coronary artery calcification (CAC).
Barrett's Esophagus
Authors: Stuart Jon Spechler
Journal: New England Journal of Medicine (2002)

A 55-year-old man has had frequent heartburn for more than 10 years. Endoscopy reveals columnar epithelium lining the distal 5 cm of the esophagus. Biopsy specimens show specialized intestinal metaplasia with inflammation and possible dysplasia. How should this patient's condition be managed?
Noninvasive Imaging of Enhanced Prostate-Specific Gene Expression Using a Two-Step Transcriptional Amplification-Based Lentivirus Vector
Authors: Meera Iyer, Felix B. Salazar, Xiaoman Lewis, Liqun Zhang, Michael Carey, Lily Wu, Sanjiv S. Gambhir
Journal: Molecular Therapy (2004)

Noninvasive evaluation of gene transfer to specific cells or tissues will allow for long-term, repetitive monitoring of transgene expression. Tissue-specific promoters that restrict the expression of a transgene to tumor cells play a vital role in cancer gene therapy imaging. In this study, we have developed a third-generation HIV-1-based lentivirus vector carrying a prostate-specific promoter to monitor the long-term, sustained expression of the firefly luciferase (fl) reporter gene in living mice. The fl gene in the transcriptionally targeted vector is driven by an enhanced prostate-specific antigen promoter in a two-step transcriptional amplification (TSTA) system. The efficiency of the lentivirus (LV-TSTA)-mediated gene delivery, cell-type specificity, and persistence of gene expression were evaluated in cell culture and in living mice carrying prostate tumor xenografts. In vivo bioluminescence imaging with a cooled charge-coupled device camera revealed significantly high levels of fl expression in prostate tumors. Injection of LV-TSTA directly into the prostate of male nude mice revealed efficient and long-term fl gene expression in the prostate tissue for up to 3 months. These studies demonstrate the significant potential of TSTA-based lentivirus vectors to confer high levels of tissue-specific gene expression from a weak promoter, while preserving cell-type specificity and the ability to image noninvasively the sustained, long-term expression of reporter genes in living animals.
Cytosolic Ca2+ concentration decreases in diabetic rat myocytes
Authors: Hideharu Hayashi, Naohisa Noda
Journal: Cardiovascular Research (1997)

Time for primary review 24 days.

Diabetes mellitus has been shown to be associated with heart failure of unknown origin, which is termed ‘diabetic cardiomyopathy’ [1]. Regan et al. [2]have shown that the small vessel lesions in diabetes mellitus have little or no relation to diabetic cardiomyopathy, suggesting that the contractile function of the diabetic cardiomyopathy was primarily defective. Although many studies have been performed to reveal cellular and subcellular derangement in diabetic cardiomyopathy [3–6], the precise mechanism is still unknown.

It has been reported that Ca2+ metabolism in diabetic myocardium has several abnormalities, including a decreased activity of Ca2+-ATPase in sarcoplasmic reticulum (SR) [7, 8]and sarcolemma [9, 10]. It has also been reported that the activity of Na+/Ca2+ exchange is lower in diabetic myocardium [10]. These reports have suggested that Ca2+ overload may be involved in the pathogenesis of diabetic cardiomyopathy [3]. Although it has been shown that certain mechanical and electrophysiological features in diabetic rats correlate well with conditions of intracellular Ca2+ overload [11, 12], direct measurement of the cytosolic Ca2+ concentration ([Ca2+]i) has not been made previously. The most popular method for measuring [Ca2+]i uses fluorescent probes such as fura-2 and indo-1 [13]. We have reported that [Ca2+]i of unstimulated rat myocytes isolated from insulin-dependent diabetes mellitus (IDDM) was lower than that of normal myocytes using fura-2 (53±3 nM in diabetic myocytes and 75±5 nM in normal myocytes, mean±s.e.; P <0.01) [14]. Recently, Lagadic-Gossmann et al. [15]also reported that basal [Ca2+]i level was lower in diabetic myocytes using indo-1. Horackova and Murphy [16]have reported that the Ca2+ content is decreased in diabetic rat … 

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National survey of use of hospital beds by adolescents aged 12 to 19 in the United Kingdom
Authors: R M Viner
Journal: BMJ (2001)

tions clinicians have adopted a more selective EEG requesting policy.Doctors may have a lower risk of making, and patients may have lower risk of receiving, a misdiagnosis of epilepsy.The reduction in the number of unnecessary procedures releases technical capacity which can be used in the conduct of other investigations.Systematic replication of this work, possibly on a regional basis, would yield savings which would permit development of accessible local neurophysiology services.Julie Jones and Clare Jowett in the audit departments of Wrexham Maelor and Royal Shrewsbury Hospitals facilitated the meetings and distribution of guidelines.We specially thank the users of the service for listening and changing their practice Contributors: DS had the original idea for the audit, helped with data collection and analysis, and is guarantor for the study.RB did most of the data collection.RP reported on all the EEGs.The paper was jointly