tbuckley/cabot-search huggingface dataset:
747 Cytokine production and bone mineral density in patients with primary biliary cirrhosis
Authors: C LEVY, P ANGULO, M NEUMAN, K LINDOR
Journal: Hepatology (2003)

A dual isotype (IgG, IgA) enzyme-linked immunosorbent assay (ELISA) designed to provide enhanced detection of primary biliary cirrhosis (PBC)-specific autoantibodies against both major mitochondrial and nuclear antigens has been developed and recently become commercially available. The assay (PBC Screen) simultaneously detects IgG and IgA autoantibodies to the immunodominant portions of the 3 major mitochondrial (MIT3) and nuclear (gp210, and sp100) antigens. The aim of this study was to compare the performance of the PBC Screen to the combined performance obtained with individual IgG ELISAs to MIT3, gp210, and sp100 on a large group of selected patients from multiple centers. A total of 1175 patients with PBC and 1232 subjects without PBC were evaluated. Non-PBC groups included healthy controls (624) as well as individuals with autoimmune hepatitis (281), primary sclerosing cholangitis (77), viral hepatitis (91 hepatitis B and 98 hepatitis C), other liver diseases (31), and other infectious or autoimmune diseases (30). The PBC Screen at the receiver operator characteristic optimized cutoff of 27.8 units, had an overall sensitivity of 83.8%, specificity of 94.7% and area under curve of 0.9212. This was similar to the specificity of 96.1% obtained by the combined results of individual MIT3, sp100, and gp210 IgG ELISAs (kappa index at 0.898). Of the 253 PBC patients without AMA detectable by immunofluorescence, 113 (44.7%) were interpreted as positive for PBC-specific autoantibodies. In conclusion, the PBC Screen is an appropriate first-line test for the diagnosis of PBC, including for patients negative for markers assessed using conventional methods.
Antifungal activity of silver nanoparticles against Candida spp.
Authors: Aleš Panáček, Milan Kolář, Renata Večeřová, Robert Prucek, Jana Soukupová, Vladimír Kryštof, Petr Hamal, Radek Zbořil, Libor Kvítek
Journal: Biomaterials (2009)
Biochemical and Molecular Studies of Mitochondrial Function in Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness
Authors: Margaret J Jackson, Laurence A Bindoff, Katharina Weber, Jonathan N Wilson, Paul Ince, K George M M Alberti, Douglass M Turnbull
Journal: Diabetes Care (1994)

OBJECTIVE To determine if diabetes insipidus, diabetes mellitus, optic atrophy, and deafness (DIDMOAD) combined with a cerebellar syndrome is associated with a systemic disorder of respiratory chain function as found in similar genetic syndromes. CASE A muscle biopsy was taken from a patient with DIDMOAD, and a mitochondrial fraction was prepared. Respiratory chain function was assessed by analysis of intermediary metabolites, histochemical analysis of muscle biopsy, measurement of the activity of individual respiratory chain complexes, oxidative flux through the respiratory chain, and cytochrome concentration and compared with a population with normal respiratory chain function. Mitochondrial DNA from skeletal muscle, brain, and pancreas was examined for major rearrangements and specific point mutations. Brain tissue was examined neuropathologically for abnormalities, particularly those previously described in association with DIDMOAD. RESULTS No abnormality was found in mitochondrial oxidation, individual complex activity, or cytochrome concentration. Histochemical analysis and electron microscopy showed no abnormality known to be associated with mitochondrial dysfunction. A single-base substitution at position 12308 of the mitochondrial genome was found, but no major rearrangement of mitochondrial DNA was demonstrated. Neuropathological examination revealed severe demyelination and gliosis in the optic nerves and loss of Purkinje cells associated with gliosis in the white matter in the cerebellum. CONCLUSIONS We have found no evidence that DIDMOAD is associated with a systemic abnormality of respiratory chain function. The mitochondrial DNA single-base substitution noted is likely to be a polymorphism rather than a pathogenic point mutation. We have confirmed that DIDMOAD may be associated with a neurodegenerative disorder, but the cause of this remains undetermined.
Evaluation of Physical Examination Skills
Authors: Diane L. Elliot
Journal: JAMA (1987)

Effective teaching of physical examination skills requires an evaluation system that can identify trainees who need further instruction. To study the evaluation of physical examination skills by faculty observers and patient instructors, the reliability of their observation of medical students performing a general physical examination was measured. Faculty observers did not reliably evaluate 32% of examination skills. Faculty agreement was lowest when observing components of the head, neck, and abdominal examinations. Patient instructors, with limited faculty training, reliably evaluated 83% of those skills that were reliably assessed by the faculty. These findings demonstrate limitations in the current methods of evaluating performance and point out the need to examine systems of trainee assessment.
P211 Fev1/fiv1 Index In Amyotrophic Lateral Sclerosis Patients
Authors: G. Kaltsakas, M. Rentzos, T. Alexakis, V. Zouvelou, I. Evdokimidis, N. Koulouris, S. Gennimata, A. Palamidas
Journal: Thorax (2014)

<h3></h3> Amyotrophic lateral sclerosis (ALS) is a relentlessly progressive, presently incurable, neurodegenerative disorder that causes muscle weakness, disability, and eventually death. The ALS Functional Rating Scale (ALSFRS-R) is a validated rating instrument for monitoring the progression of disability in patients with ALS. The ALSFRS-R incorporates questions for the assessment of dyspnoea, orthopnea, and the need for ventilatory support. However, studies on the relationship of ALSFRS-R with objective measurements of respiratory function are scanty. Therefore, we set out to investigate the relationship of ALSFRS-R with respiratory function indices in ALS patients. We studied 33 consecutive, ambulatory, Caucasian patients (21 men) with ALS. Seventeen patients had bulbar involvement. Two patients did not satisfactorily perform the lung function testing and they were excluded. ALSFRS-R was assessed. Routine lung function tests, maximum static expiratory (Pemax) and inspiratory (Pimax) mouth pressures were measured. Respiratory muscle strength (RMS) was also calculated. ALSFRS-R (mean±SD) was 35 ± 9. Patients had: age, y=60 ± 10, FEV<sub>1</sub>,%pred=83 ± 18, FVC,% pred=86 ± 20, TLC,% pred=94 ± 11, and DL<sub>CO</sub>,% pred=92 ± 18. Pemax,% pred was 80 ± 28, Pimax,% pred was 73 ± 31, and RMS,% pred was 77 ± 27. These pressures were below the normal limits in 15, 20, and 17 patients, respectively. ALSFRS-R was significantly correlated with Pemax%pred, RMS%pred, and FEV<sub>1</sub>/FIV<sub>1</sub> (r = 0.46, p &lt; 0.01; r = 0.38, p = 0.035; n = 20, r=-0.71, p &lt; 0.001, respectively). In conclusion, FEV<sub>1</sub>/FIV<sub>1 </sub>index has a good correlation with ALSFRS-R (n = 20, r=-0.71, p &lt; 0.001, FEV<sub>1</sub>/FIV<sub>1</sub> = 1.630 - [0.018 * ALSFRS-R] ± 0.165).
Blood pressure and mortality in healthy old people: the r shaped curve
Authors: J. M Starr, S. Inch, S. Cross, I. J Deary, W. J MacLennan
Journal: BMJ (1996)

Table 1-Number of emergency plans that contained specified elements of planning Arrangements specified In guidance No of plans (%) Action cards 119 (84) Standard alerting messages 67 (47) Handling relatives 123 (87) Handling press 132 (93) Liaising with police 126 (89) Handling volunteers 105 (74) Handling important visitors 35 (25) Audit of plan 58 (41) Training requirements 35 (25) Managing injured children 44 (31) All guidance 6 (4)incidents,' yet few plans specified plans for audit or the training of staff.Few plans specified procedures for the management of children, and when they were included they typically consisted of the use of a paediatrician to help in the triage and treatment of paediatric casualties.This is unlikely to be adequate in an incident resulting in large numbers of injured children.Health service guidelines state that plans should be reviewed at regional level.The small number of plans conforming with current guidance shows that this review procedure is not effective.Most hospital major incident plans need revision, and methods of reviewing plans should be strengthened in the light of these results.
Differential display and integrin alpha 6 messenger RNA overexpression in hepatocellular carcinoma
Authors: Nasim Ara Begum, Masaki Mori, Takashi Matsumata, Kenji Takenaka, Keizo Sugimachi, Graham F. Barnard
Journal: Hepatology (1995)

Our aim was to isolate potentially important differentially expressed gene products from paired human hepatocellular carcinoma (HCC) and normal liver samples using the differential messenger RNA (mRNA) display technique. Total RNA samples were reverse transcribed with anchoring oligonucleotide primers and then amplified by the polymerase chain reaction (PCR) with additional upstream random primers. Differentially expressed complementary DNA (cDNA) products were subsequently used as probes in Northern blot analysis. One such cDNA product, present in tumor but absent in normal displays, showed identity with the adhesion molecule integrin alpha 6. In Northern blots of 16 HCC pairs, the ∼5.5 kb signal of integrin alpha 6 mRNA was overexpressed in seven tumors, with a weak signal in the normal livers. For those patients with versus without integrin alpha 6 mRNA overexpression: (1) grade III (or IV) histology was noted in seven of seven versus three of nine tumors, respectively (P = .03); (2) tumor recurrence or death (at mean follow-up of 18 months) was noted in six of seven versus three of eight patients, respectively (P = .17). Similar results were obtained using semiquantitative PCR co-amplification with glyceraldehyde 3-phosphate dehydrogenase as a control; ±50% of the tumors had stronger integrin alpha 6 bands than their paired normals. Both A and B variants of integrin alpha 6 mRNA were detectable in the tumor and normal liver samples. The B variant was more pronounced than the A variant by 8.9-fold in the tumors (n = 10) compared with threefold in the normal livers (n = 10), suggesting that the overexpression of integrin alpha 6 may be more reflective of abnormalities of B variant levels than of A variant levels. Important genes whose expression correlates with significant patient variables may be isolated by differential display; based on this small series there is a trend for integrin alpha 6 mRNA to be overexpressed in high-grade HCC and to predict a tendency toward a poorer outcome. (Hepatology 1995; 22:1447–1455).
Abstract 3503: A method for quantifying soluble folate receptor 1 in IMGN853 0401 clinical trial patients.
Authors: Nathan Testa, Laura Bartle, Olga Ab, Judy Cheng, Gillian Payne, Robert J. Lutz, Ben Wolf, Christina Carrigan
Journal: Cancer Research (2013)

Abstract IMGN853, an antibody-“drug” conjugate (ADC), consists of a human folate receptor 1 (FOLR1)-binding monoclonal antibody, M9346A, conjugated to the maytansinoid, DM4, via the cleavable sulfo-SPDB linker. A phase I clinical study has been initiated to identify the maximum tolerated dose, safety, pharmacokinetics (PK), and preliminary efficacy of IMGN853 in patients with FOLR1-positive tumors, including ovarian cancer, non-small cell lung cancer, and other epithelial malignancies. The levels of soluble FOLR1 antigen (sFOLR1) in plasma may vary depending on factors such as tumor antigen expression level, tumor type, and disease stage, and may affect the PK of IMGN853and response to treatment. Previously reported studies describing the detection of sFOLR1 in patient samples are limited and not quantitative. The aim of this study was to develop a sandwich ELISA to quantify levels of sFOLR1 present in plasma samples from cancer patients for use as a potential biomarker of IMGN853 PK and/or clinical response. A panel of murine anti-FOLR1 antibodies was generated at ImmunoGen by standard hybridoma technology. Two clones were chosen for use as capture (FR1-9) and detector (FR1-13) antibodies in the ELISA due to their high affinity and ability to bind to FOLR1 even in the presence of IMGN853 or the FOLR1 ligand, folate. The extracellular domain of FOLR1 fused to murine IgG2a Fc region (FOLR1-Fc) was used as a reference standard. A sandwich ELISA was developed using FR1-9 antibody to capture sFOLR1 from patient plasma, and bound sFOLR1 was detected using biotinylated FR1-13 antibody and streptavidin-HRP with TMB as the substrate. The ELISA was used to quantify sFOLR1 in ovarian carcinoma patient plasma using a reference standard curve generated with FOLR1-Fc antigen. A robust sandwich ELISA was developed to quantify sFOLR1 in patient plasma. The quantitation range of the assay was determined to be 0.2 to 90 nanomolar. Coated plates stored frozen from 0-14 days and protein reagents freeze-thawed over two cycles gave repeatable results. The ELISA was able to detect sFOLR1 in 5 of 10 ovarian cancer patient plasma samples, where the levels of detectable sFOLR1 ranged from 0.7-31 nM. This ELISA is considered suitable to quantify levels of sFOLR1 in patients enrolled in the phase 1 trial over the course of IMGN853 treatment, and resulting data will be assessed for correlations with IMGN853 PK and with clinical response. Citation Format: Nathan Testa, Laura Bartle, Olga Ab, Judy Cheng, Gillian Payne, Robert J. Lutz, Ben Wolf, Christina Carrigan. A method for quantifying soluble folate receptor 1 in IMGN853 0401 clinical trial patients. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3503. doi:10.1158/1538-7445.AM2013-3503
Relation of global and regional left ventricular function to tomographic thallium-201 myocardial perfusion in patients with prior myocardial infarction
Authors: John R. Stratton, Sarah M. Speck, James H. Caldwell, Gary V. Martin, Manuel Cerqueira, Charles Maynard, Kathryn B. Davis, J.Ward Kennedy, James L. Ritchie
Journal: Journal of the American College of Cardiology (1988)
Effects of cyclosporin A and FK-506 on stem cell factor–induced histamine secretion and growth of human mast cells
Authors: Wolfgang R. Sperr, Hermine Agis, Klaus Czerwenka, Irene Virgolini, Hans C. Bankl, Michael R. Müller, Krisztina Zsebo, Klaus Lechner, Peter Valent
Journal: Journal of Allergy and Clinical Immunology (1996)
Percutaneous Myocardial Biopsy of the Left Ventricle
Authors: EARL K. SHIREY, WILLIAM A. HAWK, DEVOBROTO MUKERJI, DONALD B. EFFLER
Journal: Circulation (1972)

A thin-walled Silverman needle has been used for 254 percutaneous punch biopsies of the left ventricle in 198 patients with closed chests at the Cleveland Clinic. The technic is described. The biopsy specimens were adequate for diagnosis in 192 patients. In all but three patients (who had lupus erythematosus, scleroderma, and chronic glomerulonephritis with congestive heart failure) cardiac catheterization and selective cardioangiographic studies were performed. There was angiographic evidence of primary myocardial disease, coronary atherosclerosis, or both, or rheumatic valvular disease in 175 patients. Cardiac catheterization and angiographic studies demonstrated no evidence of organic heart disease in 20 patients. Cardiac tamponade was a complication of myocardial biopsy in eight patients. Post-pericardiotomy syndrome occurred in four patients and ventricular fibrillation in one patient. Myocardium with no pathologic diagnosis and interstitial fibrosis or myocardial hypertrophy or both were the light microscopic findings in 165 patients. Representative sections of the biopsy in 27 patients demonstrated small-vessel disease, basophilic degeneration, focal interstitial myocarditis, amyloidosis, Aschoff's nodules, or vacuolar degeneration. The current experience suggests that myocardial biopsy combined with selective cardioangiography is of experimental value, improves the accuracy of diagnosis, and plays a role in the management of some patients.
John Phillips Memorial Lecture: Observations on Metabolism of Aldosterone in Man.
Authors: John A. Luetscher
Journal: Annals of Internal Medicine (1963)

s1 April 1963John Phillips Memorial Lecture: Observations on Metabolism of Aldosterone in Man.John A. Luetscher, M.D.John A. Luetscher, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-58-4-719_3 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe circulating level of aldosterone reflects the balance between rate of secretion by the adrenals and metabolic clearance rate by the liver. Although the concentration of aldosterone in plasma is too low to be measured accurately, the mean plasma level can be calculated. Secretion rate is estimated from measured specific activity of a purified metabolite after injection of labeled aldosterone. In most instances, the quantity released from strongly acidified urine serves as a fair index of secretion rate; but in pregnancy, renal insufficiency, or hepatic cirrhosis, the fraction of secreted aldosterone metabolized to this form is abnormal. Metabolic clearance rate... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: John A. Luetscher, M.D.Affiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 April 1963Volume 58, Issue 4Page: 719-719KeywordsAldosteroneAttentionBlood plasmaCirrhosisLiverMetabolitesPregnancyUrine ePublished: 1 December 2008 Issue Published: 1 April 1963 PDF downloadLoading ...
Cardiac-specific effects of parathyroid hormone-related peptide: Modification by aging and hypertension
Authors: G ROSS, K SCHLUTER
Journal: Cardiovascular Research (2005)

Objective: Parathyroid hormone-related peptide (PTHrP) improves heart function of post-ischemic and stunned myocardium and is released from the heart under ischemic conditions. Hypertrophic hearts from spontaneously hypertensive rats (SHR) develop a reduced ischemic tolerance, show reduced expression of PTHrP and develop paradoxical effects in regard to PTHrP. We hypothesized that PTHrP is causally involved in reduced ischemic tolerance of hypertrophied hearts. This hypothesis was tested by addition of a cardiac-specific PTHrP agonist or antagonist during ischemia and investigation of the functional recovery during the early phase of reperfusion.
The Transcription Factor Pax6 Regulates Survival of Dopaminergic Olfactory Bulb Neurons via Crystallin αA
Authors: Jovica Ninkovic, Luisa Pinto, Stefania Petricca, Alexandra Lepier, Jian Sun, Michael A. Rieger, Timm Schroeder, Ales Cvekl, Jack Favor, Magdalena Götz
Journal: Neuron (2010)
The Medical Library as a Patient Aid-Reply
Authors: Katherine Lindner
Journal: JAMA (1992)

In Reply.  —My colleagues, Mss Rothbaum and Eisenberg, are certainly correct that I did not address the problem of financing consumer health information services. I wrote the article to increase awareness of the value of the service and to encourage physicians, hospital administrators, nurses, librarians, and the public to increase their efforts to find financial resources for consumer information services.  About 300 medical libraries in the United States have succeeded in gathering enough funds through grants, private contributions, or from their institutions to provide consumer health information services. It is up to the individual medical librarians to continually advocate resources and access for the consumer in order to develop further funds for such services.  Fortunately, the Joint Commission on Accreditation of Healthcare Organizations is revising its standards for 1994 concerning information and will recognize the importance of information support in patient care and patient education. Now is the time for
The effect of overexpression of Dlx2 on the migration, proliferation and osteogenic differentiation of cranial neural crest stem cells
Authors: Jiewen Dai, Ying Kuang, Bing Fang, Hui Gong, Shunyuan Lu, Zhifang Mou, Hao Sun, Yuefu Dong, Jingting Lu, Wenbin Zhang, Jianfei Zhang, Zhugang Wang, Xudong Wang, Guofang Shen
Journal: Biomaterials (2012)
End-systolic pressure-volume relations of isolated ejecting rabbit left ventricles after quick diastolic volume changes
Authors: P. J M KIL, P. SCHIERECK
Journal: Cardiovascular Research (1985)

Journal Article End-systolic pressure-volume relations of isolated ejecting rabbit left ventricles after quick diastolic volume changes Get access PAUL J M KIL, PAUL J M KIL From the Department of Medical Physiology, State University Utrecht, 3521 GG Utrecht, The Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar PIET SCHIERECK PIET SCHIERECK * From the Department of Medical Physiology, State University Utrecht, 3521 GG Utrecht, The Netherlands *Correspondence address and requests for reprints: P Schiereck at the above address. Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 19, Issue 12, December 1985, Pages 782–792, https://doi.org/10.1093/cvr/19.12.782 Published: 01 December 1985
Cardiopulmonary complications leading to premature deaths in adult patients with sickle cell disease
Authors: Courtney D. Fitzhugh, Naudia Lauder, Jude C. Jonassaint, Marilyn J. Telen, Xiongce Zhao, Elizabeth C. Wright, Francis R. Gilliam, Laura M. De Castro
Journal: American Journal of Hematology (2009)

Sickle cell disease (SCD) is associated with early mortality. We sought to determine the incidence, cause, and risk factors for death in an adult population of patients with SCD. All patients aged >/=18 years seen at the Adult Sickle Cell Center at Duke University Medical Center between January 2000 and April 2005 were enrolled. Forty-three patients (21 males and 22 females) died during the study period. The median age of survival was 39 years for females (95% CI: 34-56), 40 years for males (95% CI: 34-48), and 40 years overall (95% CI: 35-48). Cardiac causes of death accounted for 25.6% (11/43 patients); pulmonary, 14.0% (six patients); other SCD related, 32.6% (14 patients); unknown, 14.0% (six patients); and others, 14.0% (six patients). Pulseless electrical activity arrest, pulmonary emboli, multiorgan failure, and stroke were the most frequent causes of death. Among the deceased patients, the most common premorbid conditions were cardiopulmonary: acute chest syndrome/pneumonia (58.1%), Pulmonary hypertension (pHTN; 41.9%), systemic HTN (25.6%), congestive heart failure (25.6%), myocardial infarction (20.9%), and arrhythmias (14.0%). Tricuspid regurgitant jet velocity was significantly higher (3.1 m/sec vs. 2.6 m/sec, P < 0.001) and hemoglobin significantly lower (8.3 g/dL vs. 9.2 g/dL, P < 0.05) in deceased patients when compared with patients who lived, respectively. With improved preventive and therapeutic advances, including hydroxyurea therapy, acute complications such as infection are no longer the leading cause of death; instead, causes of death and premorbid conditions are shifting to chronic cardiopulmonary complications. Further, arrhythmia leading to premature death is under-recognized in SCD and warrants further investigation.
TCT-242 Accuracy of Optical Coherence Tomography Measurement Compared to Intravascular Ultrasound: OPUS-CLASS Study
Authors: Takashi Akasaka, Junya S. hite, Mitsuyasu Terashima, Shiro Uemura, Bo Yu, Nobuaki Suzuki, Takashi Kubo, Shaosong Zhang
Journal: Journal of the American College of Cardiology (2012)
Reason is normative, and should be studied accordingly
Authors: David Spurrett
Journal: Behavioral and Brain Sciences (2011)

Abstract Reason aims at truth, so normative considerations are a proper part of the study of reasoning. Excluding them means neglecting some of what we know or can discover about reasoning. Also, the normativist position we are asked to reject by Elqayam &amp; Evans (E&amp;E) is defined in attenuated and self-contradictory ways.