tbuckley/cabot-search huggingface dataset:
Preparation and Properties of Thyroxine-Binding Alpha Globulin (TBG)
Authors: Kenneth Sterling, Satoshi Hamada, Yoshihiro Takemura, Milton A. Brenner, Edward S. Newman, Mitsuo Inada
Journal: Journal of Clinical Investigation (1971)

Thyroxine-binding alpha globulin (TBG) in human serum was isolated from Cohn fractions IV-5,6 and IV-4 by (1) chromatography on carboxymethyl (CM) cellulose, (2) gel filtration on Sephadex G-200, (3) chromatography on diethylaminoethyl-Sephadex, (4) a novel procedure of "double-gel" electrophoresis, and (5) preparative polyacrylamide gel electrophoresis. The protein was homogeneous by analytical disc gel electrophoresis, immunoelectrophoresis, and ultracentrifugal analyses (sedimentation velocity and sedimentation equilibrium), and after addition of thyroxine-(125)I showed a constant specific radioactivity on polyacrylamide electrophoresis. The sedimentation and diffusion coefficients were s(20, w), 3.0 x 10(-13) sec, and D(20, w), 8.05 x 10(-7) cm(2).sec(-1), and the molecular weight obtained by sedimentation equilibrium was 36,500. Gel filtration studies on Sephadex G-200 demonstrated that the protein had the same elution volume as that of native TBG in serum, apparently excluding the possibility of a subunit of the native protein. Chemical composition was ascertained by amino acid and carbohydrate analyses. The maximal thyroxine (T4)-binding capacity measured by reverse flow paper electrophoresis was 15,000 mug per g of protein, representing more than 2100 times that of the starting material, or about 5000 times that of whole serum. Based on the molecular weight obtained, the TBG preparation could bind 0.7 mole T4 per mole of protein, suggesting a single binding site. The association constant for T4 was estimated to be of the order of 10(10) by competitive binding studies employing TBG and T4-binding prealbumin (TBPA).
Case of ectopic pregnancy after postcoital contraception with ethinyloestradiol-levonorgestrel.
Authors: A A Kubba, J Guillebaud
Journal: BMJ (1983)

A 34-year old para 0+1 requesting postcoital treatment 34 hours afer a single episode of unprotected intercourse on day 12 of her menstrual cycle had a history of salpingitis at age 20 and termination of pregnancy at 24.  Vaginal examination showed no abnormality.  She was given a combination of .1 mg ethinylestradiol and .5 mg levonorgestrel according to the Yuzpe regimen.  She did not experience nausea or vomiting.  At follow-up 4 weeks later she reported an episode of light vaginal bleeding which began 12 days after treatment and lasted for 8 days.  4 days afer the bleeding she began bleeding again and experienced severe lower abdominal pain.  Vaginal examination in an emergency room showed no mass or tenderness and she was discharged with no arrangement for follow-up.  The pelvic pain persisted and when she was again seen 32 days after the original treatment there was a tender left adnexal mass about 3 cm in diameter.  A left tubal pregnancy was diagnosed at laparoscopy.  The tube was distended with a hemosalpinx complicating a preexisting hydrosalpinx along with a small hemoperitoneum.  The right tube and ovary were normal.  A left salpingectomy was done and the patient made an uneventful recovery.  Because the Yuzpe regimen is thought to act primarily at the uterine level rather than to inhibit ovulation an ectopic pregnancy is more likely when the treatment fails especially if there is preexisting tubal damage.  The total number of ectopic pregnancies observed is roughly equal to what would be expected if no contraception had been used.  Patients requesting postcoital treatment should be warned of the need for prompt examination if they develop severe pelvic pain.  The history of previous tubal pregnancy should be a strong contraindication to postcoital treatment until more is known about the causative relation between postcoital hormonal treatment and ectopic pregnancy.
DIASTOLIC HYPERTENSION WITH INCREASED BASAL METABOLIC RATE
Authors: ERNST P. BOAS
Journal: JAMA (1925)

Although the cause and nature of hypertension are still unknown, certain groups of cases have been differentiated from the general mass of those with high blood pressure, and certain clinical associations have been established. Thus, it is generally accepted that patients with glomerulonephritis and hypertension, or with the vascular crises of lead, eclampsia or tabes, must be sharply distinguished from those with so-called essential hypertension. Among those belonging to the latter category, certain subgroups have been described: hypertension associated with myxedema,<sup>1</sup>and hypertension associated with tumors of the suprarenal cortex.<sup>2</sup> There is another syndrome, in which hypertension is one of the presenting signs, but in which there is a concomitant group of symptoms which serve to separate it sharply from the ordinary picture of hypertension, and which warrant its classification as a distinct disease entity. The signs and symptoms characteristic of this syndrome may briefly be enumerated as
Altered Hematopoietic Cell Gene Expression Precedes Development of Therapy-Related Myelodysplasia/Acute Myeloid Leukemia and Identifies Patients at Risk
Authors: Liang Li, Min Li, Canlan Sun, Liton Francisco, Sujata Chakraborty, Melanie Sabado, Tinisha McDonald, Janelle Gyorffy, Karen Chang, Shirong Wang, Wenhong Fan, Jiangning Li, Lue Ping Zhao, Jerald Radich, Stephen Forman, Smita Bhatia, Ravi Bhatia
Journal: Cancer Cell (2011)
Aortic Aneurysm Dissection Causing V/Q Mismatch
Authors: SERAFIN TIU, PATRICK J. TOTH, HOWARD J. BANNER, ELISSA L. KRAMER, JOSEPH J. SANGER
Journal: Clinical Nuclear Medicine (1988)

A case of unilateral mismatch on a ventilation/perfusion lung scan due to aortic aneurysm dissection is presented. Pulmonary embolism should not be considered the sole cause of unilateral lung mismatch. Clinical evaluation and pulmonary angiography should be used for a definitive diagnosis.
Diagnosis of Neuropathic vs Myopathic Disease
Authors: Joseph B. Rogoff
Journal: JAMA (1978)

To the Editor.—  A CLINICAL NOTE (239:2018, 1978) concerning renal failure following intravenous pyelography describes a patient with apparent diabetic amyotrophy. The workup is described as having been undertaken  find out the cause of his proximal amyotrophy and weight loss. Very complete laboratory investigations were carried out (including the fatal intravenous pyelogram), but the single, most important investigation necessary in any neuromuscular involvement was not done. I refer to electrodiagnostic evaluation. If the patient indeed had diabetic amyotrophy, the electrodiagnositc findings might have been of diagnostic importance.1Certainly, there is no apparent justification to have labeled the disorder a profound myopathy when the involvement more probably was neuropathic in nature.
GLYCATED HEMOGLOBIN ASSOCIATES WITH SEVERITY OF THE SYNTAX SCORE EVEN IN NON-DIABETIC ADULTS
Authors: Nobutaka Ikeda, Norihiro Kogame, Raisuke Iijima, Masaya Yamamoto, Hisao Hara, Masao Moroi, Kaoru Sugi
Journal: Journal of the American College of Cardiology (2012)
Stimulation of Fecal Steroid Excretion After Infusion of Recombinant Proapolipoprotein A-I
Authors: Mats Eriksson, Lars A. Carlson, Tatu A. Miettinen, Bo Angelin
Journal: Circulation (1999)

Background —Apolipoprotein (apo) A-I is the major protein component of HDL, a cholesterol transport particle that protects against atherosclerosis. Apo A-I is believed to promote reverse cholesterol transport, transferring cholesterol from peripheral cells to the liver for subsequent elimination. To test this hypothesis in humans, we measured fecal steroid excretion before and after the intravenous infusion of human proapo A-I (precursor of apo A-I) liposome complexes. Methods and Results —Four subjects with heterozygous familial hypercholesterolemia were studied under standardized conditions. The fecal excretion of bile acids and neutral sterols was determined for 9 days before and 9 days after an intravenous infusion of recombinant human proapo A-I (4 g protein) liposome complexes. Plasma apoA-I and HDL cholesterol levels increased transiently (mean peak concentrations were 64% and 35% above baseline, respectively) during the first 24 hours. Mean lipoprotein lipid and apolipoprotein levels were not different during the 2 collecting periods, however. Serum lathosterol, a precursor of cholesterol whose concentration reflects the rate of cholesterol synthesis in vivo, was also unchanged. The fecal excretion of cholesterol (neutral sterols and bile acids) increased in all subjects (mean increase, +39% and +30%, respectively), corresponding to the removal of ≈500 mg/d excess cholesterol after infusion. Control infusions with only liposomes in 2 of the patients did not influence lipoprotein pattern or cholesterol excretion. Conclusions —Infusion of proapoA-I liposomes in humans promotes net cholesterol excretion from the body, implying a stimulation of reverse cholesterol transport. This mechanism may prove useful in the treatment of atherosclerosis.
Anti-tumour necrosis factor monoclonal antibody treatment for ocular Behcet's disease
Authors: G Triolo
Journal: Annals of the Rheumatic Diseases (2002)

Blockade of tumour necrosis factor alpha (TNFα) using infliximab, a chimeric monoclonal antibody against TNFα, is an effective treatment in rheumatoid arthritis and Crohn's disease. 1 2 Sifikakis reported success using infliximab in sight threatening Behçet's disease. 3 A preliminary study has also reported clinical improvements in the primary systemic vasculitis, Wegener's granulomatosis, with the soluble TNFα receptor etanercept. 4 The benefit of lenercept, a soluble p55 TNFα receptor fusion protein, on digital vasculitis in rheumatoid arthritis has also been reported. 5 We report the compassionate treatment of six patients with refractory vasculitis using infliximab.Diagnoses were Wegener's granulomatosis in three and microscopic polyangiitis in three.Three patients were positive for proteinase-3 antineutrophil cytoplasmic antibodies (PR3-ANCA) and one for myeloperoxidase (MPO)-ANCA.Four were female, with a mean age of 58 years (range 23-77) and mean disease duration of 3.5 years.All had had at least three clinical relapses and had received prolonged treatment with corticosteroids and at least four immunosuppressive drugs.At the time of infliximab treatment the eyes were affected in four patients and the lung in three; in addition, five had profound constitutional symptoms.The mean prednisolone dose was 17 mg.Three intravenous doses of infliximab 200 mg were given at monthly intervals for three months.One patient complained of fatigue, myalgia, and blurred vision 24 hours after the first infusion, which did not recur on rechallenge.Infliximab was otherwise well tolerated.Five patients had remission of their disease, four within two weeks of treatment.This allowed steroid withdrawal in three and reduction by more than 50% in two.Disease activity assessed by the Birmingham Vasculitis Activity Scores (BVAS) improved from a mean of 6.3 to 0.8 at three months (fig 1). 6 One patient receiving continued infliximab for six months relapsed when the treatment interval was extended to two months.Mean falls in erythrocyte sedimentation rate and C reactive protein were 17 mm/1st h and 13 mg/l, respectively.The ANCA status was unchanged.Anti-TNFα treatment heralds a new wave of specifically targeted biological interventions of potential value in the treatment of vasculitis.It offers the hope of improved therapeutic efficacy over current agents and the possibility of reducing exposure to steroids and immunosuppressive drugs.Further studies are warranted to confirm these observations and explore the role of infliximab as a component of initial protocols.. . . . . .
TRANSFUSION OF BLOOD IN NEPHRITIS
Authors: J. Ramsay
Journal: BMJ (1920)

~~~~~~~~~~~~MEDI CAL JOUIINASI have traced the cause of several cases of enuresis to hereditary syphilis, and have cured them by a course of mercury.Skin Diseases.I lhave treated several cases of severe psoriasis with mercury.My first case caine under observation at an early age, and slhowed an excellent result:A boy of 12 or 13 had widespread psoriasis with abundant raslh.Treatmenit witli Fowler's solution in large doses caused improvement, but there were three relapses.I treated bim with mercury perchloride and he made a good recovery, having no relapse during the many years in which I continued to come into conitact wvith him.Subsequently his father consulted me for paresis of the right arm, and admitted having had a Hunterian chancre before the boy was born.
Induction of immunosuppressive molecules and regulatory T cells counteracts the antitumor effect of interleukin-12-based gene therapy in a transgenic mouse model of liver cancer
Authors: Maider Zabala, Juan José Lasarte, Christine Perret, Josu Sola, Pedro Berraondo, Maite Alfaro, Esther Larrea, Jesús Prieto, M. Gabriela Kramer
Journal: Journal of Hepatology (2007)
Contact stomatitis and lithium carbonate tablets
Authors: C. E. Muniz
Journal: JAMA (1978)

To the Editor.—  Contact stomatitis has been associated with a wide variety of metals, whether these metals are used in dental fillings and prostheses or as therapeutic agents. The metals known to produce a stomatitis include gold, mercury, bismuth, chromium, nickel, copper, and zinc. 1,2 In fact, contact stomatitis has been reported in up to 7.5% of patients treated with gold. 3   Report of a Case.—  A 36-year-old man with a 12-year history of schizo-affective schizophrenia recently complained of a sore mouth and severe burning when attempting to eat warm food or drink warm liquids. During the past four years the patient had been taking therapeutic doses of both chlorpromazine and lithium carbonate. There had been no previous history of any inflammation of the oral mucosa. When these symptoms developed, the tongue and buccal mucosa appeared to have a bright red erythema associated with several scattered erosions of the mucosa.
A human immunodeficiency caused by mutations in the PIK3R1 gene
Authors: Marie-Céline Deau, Lucie Heurtier, Pierre Frange, Felipe Suarez, Christine Bole-Feysot, Patrick Nitschke, Marina Cavazzana, Capucine Picard, Anne Durandy, Alain Fischer, Sven Kracker
Journal: Journal of Clinical Investigation (2014)

Recently, patient mutations that activate PI3K signaling have been linked to a primary antibody deficiency. Here, we used whole-exome sequencing and characterized the molecular defects in 4 patients from 3 unrelated families diagnosed with hypogammaglobulinemia and recurrent infections. We identified 2 different heterozygous splice site mutations that affect the same splice site in PIK3R1, which encodes the p85α subunit of PI3K. The resulting deletion of exon 10 produced a shortened p85α protein that lacks part of the PI3K p110-binding domain. The hypothetical loss of p85α-mediated inhibition of p110 activity was supported by elevated phosphorylation of the known downstream signaling kinase AKT in patient T cell blasts. Analysis of patient blood revealed that naive T and memory B cell counts were low, and T cell blasts displayed enhanced activation-induced cell death, which was corrected by addition of the PI3Kδ inhibitor IC87114. Furthermore, B lymphocytes proliferated weakly in response to activation via the B cell receptor and TLR9, indicating a B cell defect. The phenotype exhibited by patients carrying the PIK3R1 splice site mutation is similar to that of patients carrying gain-of-function mutations in PIK3CD. Our results suggest that PI3K activity is tightly regulated in T and B lymphocytes and that various defects in the PI3K-triggered pathway can cause primary immunodeficiencies.
Side Effects of Endobronchial Laser Treatment
Authors: R. Dierkesmann, A. Huzly
Journal: Endoscopy (1985)

Side effects that have occurred in over 250 endobronchial laser treatments are reported. The laser has been used in severe tracheal and in bronchial obstructions. In two cases significant bleeding had to be treated with tamponade. In one patient an emphysema of the mediastinum developed, in 2 further patients a small pneumothorax. Perforation of the airway was not detected and no special treatment was necessary. Three patients with life-threatening tracheal stenoses and one patient with severe stenosis of both the mainstem bronchi due to metastasis in the bifurcation lymph node had a large defect in the tracheal or bronchial wall with a deep necrosis five to twelve weeks after laser treatment; all these patients had extensive radiotherapy in addition. One patient died due to severe respiratory insufficiency, probably caused by smoke intoxication. After the laser treatment, rubber-like fibrin-rich plaques may develop, which can lead to life-threatening obstructions of the airway.
A Prospective, Randomized, Double-Blind Multicenter Trial of a Single Bolus Injection of the Novel Modified t-PA E6010 in the Treatment of Acute Myocardial Infarction: Comparison With Native t-PA
Authors: Chuichi Kawai, Yoshiki Yui, Saichi Hosoda, Masakiyo Nobuyoshi, Shin Suzuki, Hikaru Sato, Fumimaro Takatsu, Takeshi Motomiya, Katsuo Kanmatsuse, Kazuhisa Kodama, Yoshimasa Yabe, Takazo Minamino, Shin-ichi Kimata, Mitsuyoshi Nakashima
Journal: Journal of the American College of Cardiology (1997)
Acute arsenic poisoning: clinical and histopathological features
Authors: B. Bartolome, S. Cordoba, S. Nieto, J. Fernandez-Herrera, A. Garcia-Diez
Journal: British Journal of Dermatology (1999)

We report a woman with acute arsenic poisoning, who developed an erythroderma with vesicles and pustules after the ingestion of 8-16 g of sodium arsenite. Simultaneously, she presented a herpes simplex virus infection. Skin biopsies showed unique features which included multiple small pigment granules inside and outside the histiocytes. In our opinion, these findings are consistent with acute arsenic poisoning, and constitute the first histological description of this entity in skin.
Targeting the Eukaryotic Translation Initiation Factor 4E for Cancer Therapy
Authors: Jeremy R. Graff, Bruce W. Konicek, Julia H. Carter, Eric G. Marcusson
Journal: Cancer Research (2008)

The eukaryotic translation initiation factor 4E (eIF4E) is frequently overexpressed in human cancers in relation to disease progression and drives cellular transformation, tumorigenesis, and metastatic progression in experimental models. Enhanced eIF4E function results from eIF4E overexpression and/or activation of the ras and phosphatidylinositol 3-kinase/AKT pathways and selectively increases the translation of key mRNAs involved in tumor growth, angiogenesis, and cell survival. Consequently, by simultaneously and selectively reducing the expression of numerous potent growth and survival factors critical for malignancy, targeting eIF4E for inhibition may provide an attractive therapy for many different tumor types. Recent work has now shown the plausibility of therapeutically targeting eIF4E and has resulted in the advance of the first eIF4E-specific therapy to clinical trials. These studies illustrate the increased susceptibility of tumor tissues to eIF4E inhibition and support the notion that the enhanced eIF4E function common to many tumor types may represent an Achilles' heel for cancer.
Abstract 5458: Clinical and biological significance of CCR9-CCL25 axis in prostate cancer
Authors: Praveen K. Sharma, Rajesh Singh, William E. Grizzle, Shailesh Singh
Journal: Cancer Research (2010)

Abstract Bone metastasis is major cause of prostate cancer (PCa) associated deaths and handful treatment alternatives are available to treat this condition. Therefore, better understating of metastatic process and identification of molecular signature on metastatic cells are urgently needed to develop therapeutic for metastatic PCa. We have shown that CCR9, a CC chemokine receptor is highly expressed by the PCa tissue and cells and play an important role in regulating the metastatic process under the influence of its natural ligand CCL25. CCL25 is also known as a thymus-expressed chemokine (TECK) and primarily expressed by the thymus, which involutes with age. Interestingly, we have observed higher serum CCL25 in PCa patients in comparison to normal healthy donors. For the first time, we show secretion of CCL25 within the primary prostate cancer tissue in a paracrine manner. Interestingly, CCL25 was highly produced by the bone marrow cells isolated from tumor bearing mice in comparison to non-tumor bearing mice. Furthermore, inhibition of CCR9-CCL25 interaction in vivo using CCL25 neutralization antibodies, showed significant inhibition of bone metastasis after intra-cardiac injection of PC3 cells and tumor establishment after intra-tibial injection. These results provide clinical and biological significance of CCR9-CCL25 axis in prostate cancer progression and metastasis. We have also demonstrated that CCR9-CCL25 axis plays an important role in lowering the chemotherapeutic efficacy by supporting anti-apoptotic and survival signals in prostate cancer cells. Interestingly, significant inhibition in tumor burden was observed, when etoposide was given in combination CCL25 neutralization antibody in comparison to both as a single agent. Our data show strong clinical and biological significance of CCR9-CCL25 axis in prostate cancer. Furthermore, these results also show that activation of CCR9-CCL25 axis may be the possible cause behind low apoptosis rate in prostate cancer cells and could be a possible cause for unsatisfactory chemotherapeutic outcomes. Therefore, developing CCR9-CCL25 directed therapies, may improve the therapeutic outcome of prostate cancer either as a single agent or in combination with other therapeutic modalities. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5458.
Screening for the small-for-dates fetus: a two-stage ultrasonic examination schedule.
Authors: J P Nielson, C R Whitfield, T C Aitchison
Journal: BMJ (1980)

To find an effective routine screening method for small-for-dates fetuses 474 women with singleton pregnancies participated in a two-stage ultrasonic examination schedule. At the first-stage examination, which was conducted in early pregnancy, fetal crown-rump length or biparietal diameter was measured for an accurate assessment of gestational age, which was essential for interpreting the results of the second-stage examination. The second-stage examination was performed at 34-36 weeks and entailed measuring seven fetal variables, the results of which were assessed singly and in combination after delivery to identify the best indicator of small-for-dates fetuses. Fetal head measurements proved to be the least sensitive indicators of growth retardation, correctly identifying only 56-59% of cases. Measurements of trunk area and circumference, however, correctly identified 81% and 83% of cases respectively, but the most effective screening index was the product of crown-rump length and trunk area: with this index 34 out of 36 small-for-dates fetuses (94%) were correctly identified. Calculating the product of crown-rump length and trunk area from ultrasonic displays is quick and simple, and combined with the first-stage examination is a highly reliable screening method for small-for-dates fetuses.