tbuckley/cabot-search huggingface dataset:
Biliary proteins. Unique inhibitors of cholesterol crystal nucleation in human gallbladder bile.
Authors: R T Holzbach, A Kibe, E Thiel, J H Howell, M Marsh, R E Hermann
Journal: Journal of Clinical Investigation (1984)

The onset time for cholesterol crystal nucleation of supersaturated normal human gallbladder biles is consistently prolonged when compared with biles from patients with cholesterol gallstone disease. Investigation of the factor(s) responsible for the suspended supersaturation (metastability) of normal human biles revealed that model bile solutions of cholesterol saturation index (CSI) and molar lipid composition identical to individual gallbladder bile specimens had much shorter crystal nucleation times, i.e., exhibited decreased metastability. Unsaturated normal biles, after supplementation with lecithin, cholesterol, and sodium taurocholate to a 'standard' supersaturated lipid composition, also demonstrated nucleation times three- to 15-fold longer than the comparable standard model bile. Total lipid extracts of normal biles, however, when similarly supplemented, did not differ in nucleation time from the control model solution. Gallbladder biles were fractionated by gel chromatography and the eluted fractions were pooled into two fractions. The fractions eluting in about the first 25% of the included volume when mixed with the supersaturated standard model bile induced a modest increase in nucleation time of approximately 1.5 times the control value. The fractions eluting in the second 25% of the included volume and which contained all of the bile lipids, were concentrated and supplemented with lipids to the standard composition. The nucleation times of these supplements were 3-10 times longer than the control nucleation times. Delipidated bile protein mixtures, purified by discontinuous sucrose gradient centrifugation, were recombined with purified lipids at the standard composition used previously. The nucleation times of these mixtures were significantly prolonged to the same extent as those associated with the second chromatographic fraction. These observations demonstrate that the delayed onset (inhibition) of cholesterol crystal nucleation observed in normal human gallbladder bile is produced by a factor(s) present in the biliary protein fraction.
Follicular spicules of the nose: A peculiar cutaneous manifestation of multiple myeloma with cryoglobulinemia
Authors: Luis Requena, Jose Luis Sarasa, Fernando Ortiz Masllorens, Lucia Martín, Enric Piqué, Mercedes Olivares, María Carmen Fariña, Elena Prieto, Eloy Pacho, Juan Gomez Octavio
Journal: Journal of the American Academy of Dermatology (1995)
Abstract 5496: Determination of drug-kinase residence time in a homogenous, low-volume format
Authors: Bryan Marks, Connie Lebakken, Steve Riddle, Richard Somberg, Sara Hereley
Journal: Cancer Research (2010)

Abstract In addition to drug-target affinity, drug-target residence time is becoming recognized as a critical parameter influencing drug efficacy. Desired compound action can only take place when a drug is interacting with its target protein, which is governed both by the affinity of the compound for the target, as well as the dissociative off-rate of the compound from the drug-target complex. A compound with a slower dissociative off-rate may require reduced dosing schedule relative to a compound with a rapid off-rate, and effective target specificity may be increased if the off-rate for the desired drug-target complex is greater than that for off-target complexes. With respect to kinase inhibitors, a slow off-rate for Tykerb relative to the related EGFR inhibitors Tarceva and Iressa has been proposed to explained longer lasting effects in cell culture. Currently, off rates for kinases can be measured by surface plasmon resonance (SPR)-based instruments or by detailed enzyme kinetics experiments. While SPR yields highly quantitative data, it is low-throughput and requires costly instrumentation. Furthermore, it often involves covalent conjugation of protein targets to surfaces, which can lead to loss of protein function. Off-rate measurements by classical enzyme kinetics methods are established, but fairly labor-intensive. We have developed and will present data on a fully homogenous (in-solution) method for determining kinase-compound off-rates that is simple to perform in a standard fluorescence-based plate reader. In this method, a drug-kinase complex is first formed at supra-Kd concentrations of drug, and then rapidly diluted into a solution containing a fluorescent, ATP-site directed small molecule probe. As the compound dissociates from the kinase the fluorescent probe associates with the kinase, causing an increase in the fluorescence signal that can be monitored in real time. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5496.
Antidrinking campaigns target preadolescents
Authors: T. F. Kirn
Journal: JAMA (1986)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Toll-like receptor–mediated induction of type I interferon in plasmacytoid dendritic cells requires the rapamycin-sensitive PI(3)K-mTOR-p70S6K pathway
Authors: Weiping Cao, Santhakumar Manicassamy, Hua Tang, Sudhir Pai Kasturi, Ali Pirani, Niren Murthy, Bali Pulendran
Journal: Nature Immunology (2008)
Fluticasone propionate administered via metered-dose inhaler with valved holding chamber and attached facemask is effective in improving asthma control by producing at least a 2-step improvement in asthma staging in pre-school age children with asthma*1
Authors: C. Crim, C.A. Scott, C.J. Maden, W. Wu
Journal: Journal of Allergy and Clinical Immunology (2004)
Balancing medicine with a life
Authors: S. Falder
Journal: BMJ (1998)

In 1987 Glin Bennet asked: “Why are so many doctors unhappy, despite their interesting work for which they are well rewarded; and why are their patients unhappy about the care they receive?”(1) These questions are still relevant today. The high rate of suicide and drug and alcohol misuse among doctors testifies to their continuing unhappiness.(2) Stress counselling services are well used and welcome, but they have become our societyos instinctive panacea to personal problems. It is reassuring to know that there is a friendly ear to listen to our problems, but we would do better to address not only why we have these problems but what we can do to prevent them.

Eleven years on, Bennet's own answer needs revisiting. His thesis was that the wounded doctor is a better healer. He believed that doctors fail to recognise their vulnerabilities and appreciate themselves as ordinary human beings; instead, they devote their entire lives to the pursuit of medical goals and self advancement, to the detriment of patient care. By the time they reach the middle aged “plateau” and realise the lack of meaning in their lives, they are too emotionally immature to seek help. He argued that the “wound” is the doctor's eventual acknowledgment of his or her inadequacies and that accepting and dealing with these inadequacies leads not only to a happier life but also to being a better doctor.

But why do these bright, broadly gifted individuals who enter medical school fail to mature as “ordinary human beings”? Perhaps the answer lies in the authoros later statement that “most doctors have relatively simple lives in these early years, so it is possible, if they want, to give all their waking hours to the work in hand.”(3) It …
A SCHEME FOR MEDICAL BENEFIT
Authors: L. J. Picton
Journal: BMJ (1912)

FORMALIN, a 40 per cent.solution in water of the gas formaldehyde, acts, when taken internally, as an irritant poison, but its toxicity and the synmptornatology are still little known.Of the few cases described in the literature, an imbecile, aged 26, died thirty-two hours after swallow- ing between 1 and 3 oz.(30 to 89 c.cm.) of a 4 per cent.solution of formaldehyde.'Gerlach's patient," a girl, aged 21, survived a dose of 2 to.21 oz.(60 to 70 c.cnil.) of 35 per cent.formaldehyde; and Zorn's case,3 a man of 44 years, about I oz.(15 c.cm.) of a 35 to 40 per cent.solution.Klfiber I described the symptoms of a man who drank an unknown quantity of formalin and recovered.These last two cases were referred to pretty fully in the BRITISH MEDICAL JOURNAL, 1901, vol.i, EPITOnIE, Nos.42 and 300.Levison h ad a fatal case of a miian, aged 60, who lived only twenty minutes after swallowing an unknown quantity (estimaated at 2 to 3 oz.) of commercial foriualin.The case now recorded is as follows:Aimarried man, a brushmaker, aged 63 years, who had been a heavy drinker for about forty years, was off work and drinkinga cn April 15th and 16th, 1912, anid left home about 8 a.m. on April 17th without taking any breakfast.He was
ON THE NATURE OF THE HEAT-LABILE SUBSTANCE ("V") REQUIRED FOR THE GROWTH OF THE BACILLUS OF PFEIFFER
Authors: Th. Thjötta
Journal: The Journal of Experimental Medicine (1924)

It has been shown in several experiments that the heat-labile, growth-promoting factor "V" found in the blood of animals does not diminish or disappear when the animals are kept on vitamine B-free diet. The titer of the "V" substance present in the blood of normal animals is undiminished in animals showing severe signs of polyneuritis even to the time of their death. The antipolyneuritic factor of vegetable food, designated as vitamine B, is found to be considerably more heat-stable than is the "V" substance concerned in promoting the growth of Pfeiffer's bacillus.
Transgenic animals may help resolve a sticky situation in cystic fibrosis
Authors: Jonathan H. Widdicombe
Journal: Journal of Clinical Investigation (2010)

Cystic fibrosis (CF) is caused by defects in the CFTR, a cAMP-activated Cl- channel of epithelia. The resulting reduction in epithelial fluid transport creates abnormally viscous secretions from airway mucous glands that may be a major factor in CF pathology. Mouse airways have few mucous glands, and the mouse model of CF exhibits no significant airway disease. Pigs and ferrets, however, have approximately the same number of airway mucous glands as humans. In this issue of the JCI, three independent research groups conclude that changes in airway mucous gland function in CFTR-deficient animals of these species resemble the changes seen in human CF. It is expected, therefore, that these animals will develop lung disease similar to human CF and prove to be valuable models on which to test potential therapies.
Molecular evolution of glutamate receptors: a primitive signaling mechanism that existed before plants and animals diverged
Authors: J. Chiu, R. DeSalle, H. M. Lam, L. Meisel, G. Coruzzi
Journal: Molecular Biology and Evolution (1999)

We performed a genealogical analysis of the ionotropic glutamate receptor (iGluR) gene family, which includes the animal iGluRs and the newly isolated glutamate receptor-like genes (GLR) of plants discovered in Arabidopsis. Distance measures firmly placed the plant GLR genes within the iGluR clade as opposed to other ion channel clades and indicated that iGluRs may be a primitive signaling mechanism that predated the divergence of animals and plants. Moreover, phylogenetic analyses using both parsimony and neighbor joining indicated that the divergence of animal iGluRs and plant GLR genes predated the divergence of iGluR subtypes (NMDA vs. AMPA/KA) in animals. By estimating the congruence of the various glutamate receptor gene regions, we showed that the different functional domains, including the two ligand-binding domains and the transmembrane regions, have coevolved, suggesting that they assembled together before plants and animals diverged. Based on residue conservation and divergence as well as positions of residues with respect to functional domains of iGluR proteins, we attempted to examine structure-function relationships. This analysis defined M3 as the most highly conserved transmembrane domain and identified potential functionally important conserved residues whose function can be examined in future studies.
Serum from patients with restenosis after PTCA stimulates proliferation of bovine vascular smooth muscle cells under low extracellular calcium condition
Authors: M. Shirotani, Y. Yui, R. Hattori, M. Takahashi, T. Aoyama, Y. Murohara, H. Morishita, K. Kadota, C. Kawai
Journal: Journal of the American College of Cardiology (1991)
Endoscopie Procedures Series.
Authors: DONALD H. KUIPER
Journal: Annals of Internal Medicine (1984)

Audiovisual Materials1 August 1984Endoscopie Procedures Series.DONALD H. KUIPER, M.D.DONALD H. KUIPER, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-101-2-285_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe grandview osteopathic hospital staff has prepared a color audiovisual cassette to be used for patient self-instruction in preparation for endoscopy. Concise instructions are given for the inpatient setting, such as the fact that the patient will receive an injection the evening before the examination. There is an excellent description of the light source and the fiberoptic endoscope. The live patient model appears unkempt and disheveled, and this might be distressing to other patients. Drawings and diagrams are used to show endoscopic biopsy and polypectomy technique. The section on endoscopic retrograde cholangiopancreatography is particularly well done, but that on colonoscopy... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byCorrection: Review of Videotapes on EndoscopyJACK ROUTLEDGE 1 August 1984Volume 101, Issue 2Page: 285-286KeywordsBiopsyColonoscopyEndoscopic retrograde cholangiopancreatographyEndoscopyInpatientsPolypectomies Issue Published: 1 August 1984 PDF downloadLoading ...
Expression and activation of pro-MMP-2 and pro-MMP-9 during rat liver regeneration
Authors: Tae-Hyoung Kim, Wendy M. Mars, Donna B. Stolz, George K. Michalopoulos
Journal: Hepatology (2000)

Partial hepatectomy triggers a variety of biological phenomena, which culminate in regeneration of the liver mass. Hepatocyte proliferation is a major feature of the regenerating liver after partial hepatectomy. Previous studies in our laboratory suggested that hepatic matrix remodeling might be a prerequisite process for hepatocyte proliferation in the regenerating liver. In the present study we use immunohistochemical staining, Western blot analysis, and gelatin zymography to show that the inactive matrix metalloproteinases (MMPs), pro-MMP-2 and pro-MMP-9, are elevated at 30 minutes and activated at 6 to 12 hours and at 3 to 6 hours, respectively, after hepatectomy. Sham-operated livers did not show an increase in inactive pro-MMP-2 and pro-MMP-9 and did not contain active MMP-2 or MMP-9. To examine whether tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) is changed to regulate the activities of MMPs after partial hepatectomy, the level of TIMP-1 protein was analyzed by both immunohistochemistry and Western blot analysis. The level of TIMP-1 protein appears to increase by 6 to 18 hours, implying that an increase in TIMP-1 regulates activities of active MMP-2 and MMP-9. Taken together, these results suggest that hepatic matrix remodeling is mediated by activated MMPs, which contribute to modulation of the environment surrounding hepatocytes during rat liver regeneration.
A Simple "Curve-fit" Method of Calculating Area Under the Terminal Downslope of Indicator Dilution Curves
Authors: D. A. Killen, R. France
Journal: Cardiovascular Research (1969)

A simple visual method by which the decay constant of indicator dilution curves can be determined is described. The area under the terminal downslope is calculated from the appropriate decay constant eliminating the need to replot the downslope on semilogarithmic paper. The method saves time and is accurate.
Inguinal Hernia: A Handicapping Condition?-Reply
Authors: T. Forrest Fisher
Journal: JAMA (1983)

<h3>In Reply.—</h3> I would agree with Dr Berliner that company policy should recognize the need for individual consideration in each case. For this reason, the occupational physician should have a leadership role in the development of company policy in the medical area. The fact that a medical condition is determined to be a handicap does not dictate that correction of the handicap must be undertaken or that placement at work is impossible without correction of the handicap. The occupational physician should make appropriate recommendations to management for modification of activities and exposures based on the findings of the individual's medical evaluation. There is no need to disclose a specific medical diagnosis or handicap to management. Confidentiality should be maintained in each case.
Screening for Hypertension in the Emergency Department
Authors: Roger I. M. Glass
Journal: JAMA (1978)

Review of emergency department charts at three university-affiliated hospitals showed that less than half of all patients who had elevated blood pressure (BP) recorded were recognized by physicians to be hypertensive. At the primary teaching hospital, less than one third of patients with the greatest BP elevation (&gt;20 mm Hg above normal) were sent for some type of hypertension follow-up care. (<i>JAMA</i>240:1973-1974, 1978)
Advice From Some Health Food Stores
Authors: Sheldon S. Stoffer
Journal: JAMA (1980)

To the Editor.—  We have seen increasingly large numbers of our patients taking preparations of sometimes dubious value purchased from the health food industry. Our interest was aroused when one of our patients was advised to discontinue her regimen of digoxin, a diuretic, and levothyroxine sodium by a nutritionist at a health food store and was further advised to take kelp and several vitamin supplements instead.  This incident caused us to wonder about the nature of information being provided by the health food industry. We surveyed ten health food distributors by having some of our employees seek out the supervisor or nutritionist of each establishment. Our investigators mentioned that their physician was treating them with thyroid hormone because they had a goiter and asked if they had any products that would help. All ten advised that they had such useful products. Two advised stopping treatment with thyroid hormone, six advised
Lower Gastrointestinal Bleeding Diagnosed by Red Blood Cell Scintigraphy in a Patient With a Left Ventricular Assist Device
Authors: Mark Tulchinsky
Journal: Clinical Nuclear Medicine (2008)

A patient with ischemic congestive heart failure, managed with a left ventricular assist device (LVAD) as a bridge to cardiac transplantation, developed hematochezia. This article illustrates the utility of Tc-99m-labeled red blood cell scintigraphy for identification of the lower gastrointestinal bleeding site in a patient with LVAD. The study initially showed a normal biodistribution of radiotracer, with expected activity in the sac of the LVAD and its cannulae, and later identified the bleeding from the sigmoid, permitting selective angiography and therapeutic embolization. There is a prior limited illustration of red blood cell scintigraphy in the presence of LVAD.
Combination of vitamin K2 and angiotensin-converting enzyme inhibitor ameliorates cumulative recurrence of hepatocellular carcinoma
Authors: Hitoshi Yoshiji, Ryuichi Noguchi, Masahisa Toyohara, Yasuhide Ikenaka, Mitsuteru Kitade, Kosuke Kaji, Masaharu Yamazaki, Junichi Yamao, Akira Mitoro, Masayoshi Sawai, Motoyuki Yoshida, Masao Fujimoto, Tatsuhiro Tsujimoto, Hideto Kawaratani, Masahito Uemura, Hiroshi Fukui
Journal: Journal of Hepatology (2009)
Case 20-1982
Authors: Richard C. Cabot, Robert E. Scully, Eugene J. Mark, Betty U. McNeely
Journal: New England Journal of Medicine (1982)

Presentation of Case A 42-year old woman was admitted to the hospital because of dyspnea and edema. There was a history of ulcerative colitis of 19 years' duration. Several episodes of activity had been treated with adrenocorticosteroids and sulfasalazine. Two months before entry she began to have bouts of irregular cardiac rhythm that lasted 15 to 30 seconds. A physician found no abnormality on physical examination. An electrocardiogram revealed incomplete right-bundle-branch block. Periodic sigmoidoscopic examination disclosed evidence of mildly active colitis. The patient was advised to discontinue caffeine and felt improved. One month before admission she became fatigued and experienced . . .
Diurnal variability---time to change asthma guidelines?
Authors: H. Reddel, C. Jenkins, A. Woolcock
Journal: BMJ (1999)

Peak expiratory flow varies throughout the day in normal subjects, and this diurnal variation is increased in people with asthma.1 Current asthma guidelines recommend that diurnal variability of the peak expiratory flow rate should be calculated when diagnosing asthma and assessing its severity,2–7 including during exacerbations.34 Diurnal variability of peak flow has been used as a marker of airway responsiveness,8 9 particularly in epidemiological studies,10 11 and as an outcome measure in clinical asthma trials.12 However, there are problems associated with its use.

#### Summary points

Variation in peak flow over days or weeks provides helpful information about asthma control

Asthma guidelines recommend that diurnal peak flow variability is calculated to provide an index of airway lability

These calculations are too time consuming for normal clinical practice

Factors such as the time of recording or recent use of β2 agonist drugs result in minor changes in peak flow, but can cause large errors in diurnal variability

Diurnal variability may fail to detect important changes in lung function

An alternative, simpler index of peak flow variation such as the lowest morning peak flow expressed as percentage of the patient's personal best peak flow value should be evaluated for inclusion in asthma guidelines

Although diurnal variability in peak flow has been included in asthma guidelines for many years, doctors in primary and secondary care settings rarely use it, because of the cumbersome calculations involved. Several alternative equations may be used. The most common are the amplitude percentage mean ((maximum—minimum)/mean) or the amplitude percentage maximum ((maximum—minimum)/maximum), calculated for each day, and then averaged over a period of 1 to 2 weeks.13 Determining the amplitude percentage mean from as few as 7 days of twice daily peak flow readings for one patient (see fig 1) is …