tbuckley/cabot-search huggingface dataset:
A8.15 The Focal Contact Protein Lasp-1 Modulates the Migration Capacity of Synovial Fibroblasts
Authors: Adelheid Korb-Pap, Denise Beckmann, Jan Hillen, Marianne Heitzmann, Catherine S Chew, Stefan Butz, Dietmar Vestweber, Hermann Pavenstädt, Thomas Pap
Journal: Annals of the Rheumatic Diseases (2013)

<h3>Background and Objectives</h3> RA synovial fibroblasts (SF) have been suggested to contribute to the spreading of disease through their ability to leave cartilage destruction sites, migrate via the bloodstream and re-initiate the destructive process at distant articular cartilage surfaces. In this context, the actin-crosslinking protein Lasp-1 is of interest, because it is localised at leading edges of migrating cells and regulates metastatic dissemination of different tumours. Therefore, it is particularly important to investigate the role of Lasp-1 in SF migration and its effects on RA. <h3>Materials and Methods</h3> To identify different Lasp-1 expression levels in the hind paws of wt and hTNFtg mice, an established model for human RA, Western- blot analyses were performed. In parallel, Lasp-1 expression and its sub-cellular distribution was investigated in SF from wt and hTNFtg mice by Western-blot analyses and immunofluorescence. The migratory capacity of SFs derived from wild-type, Lasp-1<sup>-/-</sup>, hTNFtg and Lasp1<sup>-/-</sup>/hTNFtg mice was studied in a modified scratch assay as well as in live cell imaging studies. Furthermore, a transmigration assay using SF from all four genotypes and murine endothelioma cells (bEnd.5) as an endothelial barrier was carried out. For more detailed information, SF transmigration was evaluated when endothelial cells were also pre-treated with TNF-alpha, mimicking inflammatory conditions. <h3>Results</h3> Lasp-1 expression is upregulated in SF from hTNFtg mice and localises to structures of cell adhesion and invasion. In the scratch assay, a significantly reduced migration rate was detected in Lasp-1<sup>-/-</sup> SFs after 24 hrs (–43.7% versus wt, p &lt; 0.05) and in Lasp1<sup>-/-</sup>/hTNFtg, respectively (–69.11% versus hTNFtg, p &lt; 0.05). Live cell imaging studies showed a slower migration and striking differences in migration morphology of Lasp1<sup>-/-</sup>/hTNFtg compared to hTNFtg SF. Furthermore, analyses showed a significant reduction of transmigration of Lasp1<sup>-/-</sup>/hTNFtg compared to hTNFtg SF that was even enhanced by TNF-alpha stimulation of the endothelial cells. Interestingly, interbred Lasp1<sup>-/-</sup>/hTNFtg mice presented milder clinical symptoms and analyses of histopathology revealed less cartilage degradation and less attachment of synovial tissue to the cartilage than hTNFtg mice at an age of 14 weeks. <h3>Conclusions</h3> Our data provide that the migratory capacity of SF is regulated by Lasp-1 and influences the severity of arthritis in hTNFtg mice. SF – when activated – migrate through the formation of invasive and adhesive membrane structures such as invadopodia, where Lasp-1 is prominently localised. Thus, targeting Lasp-1 may be a promising strategy to reduce the invasive and migratory behaviour of synovial fibroblasts in RA.
FAB L3 Type of B‐cell acute lymphoblastic leukemia (B‐ALL) without chromosome abnormalities
Authors: Nobutaka Imamura, Deodatus M. Mtasiwa, Hiromi Ota, Tominari Inada, Atsushi Kuramoto
Journal: American Journal of Hematology (1990)

Abstract Acute lymphocytic leukemias (ALLs) are morphologically classified into L1, L2, and L3. The former two types are phenotypically constituted of quite heterogeneous ALLs. In the present study, phenotypes of cells from five L3 type ALL were analysed in FACS‐IV using a panel of monoclonal antibodies (MAbs). The leukemic cells of all these patients coexpressed la (HLA‐DR), CD19, CD20, CD21, CD24, CD38, and surface immunoglobulins, whereas a negative reaction with MAbs CD1, CD2, CD3, CD4, CD7, CD8, and Ti (WT31), TiγA, δTCS 1, and anti TCR‐γ/δ was observed. Neither myeloid‐monocyte‐erythroid nor megakaryocyte related cell surface antigens were detected in these cases with L3 type ALL. Chromosomal analysis of the ALL cells from two cases revealed a normoploid karyotype with specific translocation t(8;14)(q24;q32), whereas it was normal (46XY or 46XX) for the remaining three cases. Expression of myc oncogene was high in the former group, but low in the later one. Basing from our findings, we conclude that L3 type ALL is heterogeneous with respect to immunophenotypes, cytogenetics and oncogene analysis.
The Competition Strategy
Authors: Alain C. Enthoven
Journal: New England Journal of Medicine (1981)

The 96th Congress saw the introduction of five procompetition bills for reform of the nation's health-services system. In order of appearance, their authors were Senator David Durenberger (R.-Minn.), a member of the Health Subcommittee of the Finance Committee,1 Senator Richard Schweiker (R.-Penn.), ranking minority member of the Labor and Human Resources Committee,2 Representative Al Ullman (D.-Ore.), Chairman of the Ways and Means Committee,3 Representatives Richard Gephardt (D.-Mo.) of the Ways and Means Committee and David Stockman (R.-Mich.) of the Health and Environment Subcommittee of the Interstate and Foreign Commerce Committee,4 and Representatives James Jones (D.-Okla.) and Sam Gibbons (D-Fla.) of . . .
How Could Antidepressants Worsen Unipolar Depression?
Authors: Franco Benazzi
Journal: Psychotherapy and Psychosomatics (2003)

Letters| March 03 2003 How Could Antidepressants Worsen Unipolar Depression? Subject Area: Psychiatry and Psychology Franco Benazzi Franco Benazzi Outpatient Psychiatry Center, University of California, San Diego, Calif., USA; Collaborating Center, Ravenna and Forlì; Department of Psychology, University of Bologna, and Department of Psychiatry, National Health Service of Forlì, Italy Search for other works by this author on: This Site PubMed Google Scholar Psychother Psychosom (2003) 72 (2): 107–108. https://doi.org/10.1159/000068683 Article history Published Online: March 03 2003 Content Tools Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Franco Benazzi; How Could Antidepressants Worsen Unipolar Depression?. Psychother Psychosom 1 April 2003; 72 (2): 107–108. https://doi.org/10.1159/000068683 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsPsychotherapy and Psychosomatics Search Advanced Search This content is only available via PDF. 2003Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Article PDF first page preview Close Modal You do not currently have access to this content.
Effects of the angiotensin converting enzyme inhibitor enalapril on the long-term progression of left ventricular dysfunction in patients with heart failure. SOLVD Investigators.
Authors: M A Konstam, M F Rousseau, M W Kronenberg, J E Udelson, J Melin, D Stewart, N Dolan, T R Edens, S Ahn, D Kinan
Journal: Circulation (1992)

BACKGROUND In patients with heart failure, activation of the renin-angiotensin system is common and has been postulated to provide a stimulus for further left ventricular (LV) structural and functional derangement. We tested the hypothesis that chronic administration of the angiotensin converting enzyme (ACE) inhibitor enalapril prevents or reverses LV dilatation and systolic dysfunction among patients with depressed ejection fraction (EF) and symptomatic heart failure. METHODS AND RESULTS We examined subsets of patients enrolled in the Treatment Trial of Studies of Left Ventricular Dysfunction (SOLVD). Fifty-six patients with mild to moderate heart failure underwent serial radionuclide ventriculograms, and 16 underwent serial left heart catheterizations, before and after randomization to enalapril (2.5-20 mg/day) or placebo. At 1 year, there were significant treatment differences in LV end-diastolic volume (EDV; p less than 0.01), end-systolic volume (ESV; p less than 0.005), and EF (p less than 0.05). These effects resulted from increases in EDV (mean +/- SD, 136 +/- 27 to 151 +/- 38 ml/m2) and ESV (103 +/- 24 to 116 +/- 24 ml/m2) in the placebo group and decreases in EDV (140 +/- 44 to 127 +/- 37 ml/m2) and ESV (106 +/- 42 to 93 +/- 37 ml/m2) in the enalapril group. Mean LVEF increased in enalapril patients from 0.25 +/- 0.07 to 0.29 +/- 0.08 (p less than 0.01). There was a significant treatment difference in LV end-diastolic pressure at 1 year (p less than 0.05), with changes paralleling those of EDV. The time constant of LV relaxation changed only in the placebo group (p less than 0.01 versus enalapril), increasing from 59.2 +/- 8.0 to 67.8 +/- 7.2 msec. Serial radionuclide studies over a period of 33 months showed increases in LV volumes only in the placebo group. Two weeks after withdrawal of enalapril, EDV and ESV increased to baseline levels but not to the higher levels observed with placebo. CONCLUSIONS In patients with heart failure and reduced LVEF, chronic ACE inhibition with enalapril prevents progressive LV dilatation and systolic dysfunction (increased ESV). These effects probably result from a combination of altered remodeling and sustained reduction in preload and afterload.
Evidence for a splanchnic sodium input monitor regulating renal sodium excretion in man. Lack of dependence upon aldosterone.
Authors: R M Carey
Journal: Circulation Research (1978)

Eight normal male subjects were placed on a constant 10 mEq sodium, 60 mEq potassium diet for 5 days. At 8:00 a.m. on the 5th day, the subjects were given a standard dose of 100 mEq of sodium orally or intravenously. Subjects receiving oral sodium also received 200ml of 5% dextrose in water intravenously, and those receiving intravenous sodium also received placebo capsules orally. Water intake and posture were controlled. The subjects then returned to a free diet for 1 month and subsequently were restudied by using the opposite route of sodium administration. The subjects given the oral sodium load excreted greater quantities of sodium in their urine than those repleted intravenously. The differential natriuresis was significant as early as 2 hours after sodium loading. Plasma aldosterone concentration was similar irrespective of the route of sodium administration. Six patients with primary adrenocortical insufficiency and documented hypoaldosteronism were studied with the same protocol after 5 days of 50 mEq sodium, 60 mEq potassium intake. They also had significantly greater natriuresis after oral than intravenous sodium administration. The data suggest the presence of a splanchnic input monitor for sodium which partially regulates renal sodium excretion and is not dependent upon a turn-off mechanism for aldosterone secretion.
892. GeneJammer® Enhances Cellular Transduction with Recombinant Adenovirus
Authors: Christine M. Dilling, Pablo Bosh, Alan R. Davis, Jessica A. Shafer, Steven Stice, Malavosklish Bikram, Jennifer L. West, Elizabeth A. Olmsted-Davis
Journal: Molecular Therapy (2005)

Viral vectors are extensively used to deliver exogenous DNA in eukaryotic cells for a broad range of applications from basic research to potential clinical uses. Adenoviruses are particularly attractive vectors due to their ability to infect a wide range of cell types. A limiting step in recombinant adenovirus uptake in certain cell types is the internalization into the target cells which is mediated by membrane receptors. Although polyamines have been widely studied regarding their ability to enhance transfer of DNA into cells, little is known about their potential role in viral transduction of cells.
Wholemeal versus wholegrain breads: proportion of whole or cracked grain and the glycaemic response.
Authors: D. J. Jenkins, V. Wesson, T. M. Wolever, A. L. Jenkins, J. Kalmusky, S. Guidici, A. Csima, R. G. Josse, G. S. Wong
Journal: BMJ (1988)

STUDY OBJECTIVE--To determine the effect on the glycaemic response to bread of the ratio of whole cereal grains to milled flour. DESIGN--Randomised assignment of groups of diabetic volunteers to test and control meals, taken after an overnight fast. Test foods were also analysed for in vitro digestion with human saliva. SETTING--Tertiary care centre. PATIENTS--Groups of six drawn from pool of 16 volunteers with diabetes mellitus (11 men, five women; mean age 64 (SE 3); 10 taking insulin, five taking oral agents, one controlled by diet; other characteristics comparable). INTERVENTIONS--All patients took standard white bread control meals on three occasions spanning the study and on different mornings took test meals containing varying ratios of whole cereal grains (barley or cracked wheat) to milled flour (75:25, 50:50, 0:100). All meals contained 50 g available carbohydrate and were eaten in 15 minutes. Capillary blood samples were taken for determination of glucose concentrations every 30 minutes for three hours. END POINT--Glycaemic index of foods (= increase in area under blood glucose concentration curve for test food divided by increase in area under curve for white bread control X 100). MEASUREMENTS AND MAIN RESULTS--Significant trend to lower glycaemic index with increasing proportion of whole cereal grains in test bread (p less than 0.05) and lower in vitro digestibility (p less than 0.001). Breads containing up to 75% whole grain were considered palatable. CONCLUSIONS--Breads containing a high proportion of whole cereal grains may be useful in reducing the postprandial blood glucose profile in diabetics because they are more slowly digested. These breads should be called "wholegrain" in distinction to "wholemeal" breads made from milled flour.
Improved Adeno-Associated Virus Vector Production with Transfection of a Single Helper Adenovirus Gene, E4orf6
Authors: James M. Allen, Christine L. Halbert, A.Dusty Miller
Journal: Molecular Therapy (2000)

Recent advances in adeno-associated virus (AAV) vector production have eliminated the need for adenovirus infection by transfection of plasmids encoding the adenovirus E2A, E4orf6, and VA RNA transcription units. We report here the generation of significantly higher AAV vector titers with transfection of the single adenovirus gene, E4orf6, when used in conjunction with the split AAV packaging plasmids MTrep and CMVcap. Transduction in a murine lung model with these higher titer vector stocks was greater than that observed with traditional preparation methods. The generation of higher titer AAV vector stocks with fewer adenovirus gene products and free of replication-competent AAV will enhance the potential for AAV in clinical applications.
The Effect of Erythrocyte Lipoprotein Complexes on Thromboplastin Generation in Normal and Diseased States.
Authors: Leonard J. Stutman
Journal: Annals of Internal Medicine (1966)

s1 May 1966The Effect of Erythrocyte Lipoprotein Complexes on Thromboplastin Generation in Normal and Diseased States.Leonard J. Stutman, M.D., George Y. Shinowara, Ph.D.Leonard J. Stutman, M.D.Search for more papers by this author, George Y. Shinowara, Ph.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-64-5-1163_3 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThrombosis and an increased rate of hemolysis are major features of SS sickle cell disease. Previous investigations from this laboratory have implicated the release of erythrocyte lipoprotein and the increased rate of hemolysis in sickle cell crisis. Lipoprotein complexes were isolated from blood drawn via the citrate-in-syringe technique from five normal people, five people with sickle cell (SS) disease, one person with SC disease, and three essential hyperlipemics. The blood was centrifuged at 1,650 times gravity for 30 minutes at 1 C, and subsequently the red cells were washed three times at 1 C with buffered saline, pH 7.4. Aliquots... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: New York, New York PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 May 1966Volume 64, Issue 5Page: 1163-1164KeywordsBlood cellsLipoproteinsResearch laboratoriesSickle cell diseaseThrombosis Issue Published: 1 May 1966 PDF DownloadLoading ...
Adhesion Pain and The Peritoneoscopist
Authors: Eddy D. Palmer
Journal: JAMA (1967)

<h3>To the Editor:—</h3> Uncertainty over how much abdominal pain can properly be blamed on peritoneal adhesions has added to the difficulties of abdominal diagnosis for years. It is my understanding that in most centers undergraduate teaching continues to deemphasize adhesions as a source of chronic and recurrent pain. Certainly surgical lysis of adhesions for any reason other than bowel obstruction continues to be in disfavor. The matter is extraordinarily difficult to study in any precise way. The peritoneoscopist is likely to be a believer in adhesion pain, provided it is carefully and conservatively defined. Certainly, at least, he is in a good position to judge adhesion sensitivity in the individual patient: he can manipulate and stretch certain adhesions under direct vision, simultaneously observing the subjective pain response. Only adhesions that have one attachment to the anterior abdominal wall or to the anterior portion of the diaphragm can be so studied.
Activated regulatory T cells are the major T cell type emigrating from the skin during a cutaneous immune response in mice
Authors: Michio Tomura, Tetsuya Honda, Hideaki Tanizaki, Atsushi Otsuka, Gyohei Egawa, Yoshiki Tokura, Herman Waldmann, Shohei Hori, Jason G. Cyster, Takeshi Watanabe, Yoshiki Miyachi, Osami Kanagawa, Kenji Kabashima
Journal: Journal of Clinical Investigation (2010)

Tregs play an important role in protecting the skin from autoimmune attack. However, the extent of Treg trafficking between the skin and draining lymph nodes (DLNs) is unknown. We set out to investigate this using mice engineered to express the photoconvertible fluorescence protein Kaede, which changes from green to red when exposed to violet light. By exposing the skin of Kaede-transgenic mice to violet light, we were able to label T cells in the periphery under physiological conditions with Kaede-red and demonstrated that both memory phenotype CD4+Foxp3– non-Tregs and CD4+Foxp3+ Tregs migrated from the skin to DLNs in the steady state. During cutaneous immune responses, Tregs constituted the major emigrants and inhibited immune responses more robustly than did LN-resident Tregs. We consistently observed that cutaneous immune responses were prolonged by depletion of endogenous Tregs in vivo. In addition, the circulating Tregs specifically included activated CD25hi Tregs that demonstrated a strong inhibitory function. Together, our results suggest that Tregs in circulation infiltrate the periphery, traffic to DLNs, and then recirculate back to the skin, contributing to the downregulation of cutaneous immune responses.
Ice-Water Enteroclysis in Typhoid Fever.
Authors: J. S. Tracy
Journal: JAMA (1898)

Toledo, Ohio, Aug. 1, 1998. <h3>To the Editor:</h3> —After having had favorable results with icewater flushings of the bowels in the lowering of high temperatures in typhoid fever, I have wondered that greater stress is not laid upon the use of the measure by those who write upon the treatment of that disease. The cases are few in number in which I have had the opportunity to test the practice, and it may be that these few cases were such as would have done well under the Brand method, the wet pack, or any other plan of refrigeration; indeed, such is doubtless true. For several reasons though, my practice, limited as it is, leads me to look upon ice-water enteroclysis as superior to any other method of lowering the temperature which I have seen used. In the first place, fever patients need much more water than they in their semiconscious
Perceiving and describing motion events
Authors: Shulan Lu, Donald R. Franceschetti
Journal: Behavioral and Brain Sciences (2003)

According to Hurford, PREDICATE (x) is correlated with deictic object variables during event perception. This claim is inconsistent with some core literature on the perception of motion events. We point out that the perception of events involves the activation of the modal properties and amodal properties of underlying event structure, for which Hurford's target article fails to account.
Update in Asthma 2010
Authors: Shamsah Kazani, Elliot Israel
Journal: American Journal of Respiratory and Critical Care Medicine (2011)

The prevalence of asthma has been increasing, especially in children (1). An article reported that in Canada one in three patients would have a physician diagnosis of asthma in their lifetime (2). Work in the field of asthma this year included evaluation of new therapies, reevaluation of the benefits and risks of available therapy, and research into clinical and biomarker patterns that might allow us to differentiate among different phenotypes of asthma. There were also reports on advances in the understanding of potential immune, cellular, and biochemical mechanisms that might contribute to asthma pathobiology, work in defining genetic contributors to asthma, and elucidation of environmental factors that can influence the development or expression of the disease.
A Comparison of Eating Behaviors in Newly Diagnosed NIDDM Patients and Case-Matched Control Subjects
Authors: Justin Kenardy, Melba Mensch, Kerry Bowen, Sallie-Anne Pearson
Journal: Diabetes Care (1994)

OBJECTIVE To determine whether disordered eating may be problematic in non-insulin-dependent diabetes mellitus (NIDDM). RESEARCH DESIGN AND METHODS We contrasted the eating behaviors and attitudes in 50 newly diagnosed NIDDM patients with 50 age-, sex-, and weight-matched control subjects. RESULTS Although 14% of diabetic subjects versus 4% of nondiabetic subjects reported episodes of binge eating (P &amp;lt; 0.10), there was no difference between diabetic and nondiabetic subjects in the prevalence with which they met criteria for binge eating disorder. Diabetic patients with a history of binge eating were significantly heavier, had younger age at diagnosis, and had more problems with eating in response to situational and emotional cues than did diabetic patients who did not binge. CONCLUSIONS No support was found for greater prevalence of binge eating disorder in newly diagnosed NIDDM patients than in matched nondiabetic control subjects.