tbuckley/cabot-search huggingface dataset:
Abstract 3840: Development of an innovative 3D cell culture system to study tumor-stroma interactions in cancer cells.
Authors: Arno Amann, Marit Zwierzina, Gabriele Gamerith, Mario Bitsche, Julia M. Huber, Georg Vogel, Michael Blumer, Stefan Koeck, Jens Kelm, Wolfgang Hilbe, Heinz Zwierzina
Journal: Cancer Research (2013)

Abstract Introduction: We describe a novel 3D co-culture method using non-small cell lung cancer (NSCLC) cell lines in combination with a lung fibroblast cell line in the hanging drop technology. This method allows the investigation of tumour-stroma interactions and addresses the importance of having a more in vivo like cell culture model. Compared to other 3D models, it better reflects tumour growth in its microenvironment since no addition of growth factors or artificial extracellular matrix (ECM) substitutes are required. Methods: 96-well hanging drop microtiter plates (InSphero AG, Zürich, Switzerland) were used for the production of 3D mono- and co-cultures. In these hanging drops the two NSCLC cell lines A549 and Colo699 were cultivated either alone or co-cultured with lung fibroblasts. The viability of tumour spheroids was confirmed after five and ten days by using Annexin V/Propidium Iodide staining for flow-cytometry. Tumour fibroblast spheroid aggregation was displayed either by scanning electron microscope, semi-thin sections, immunofluorescence or by immunohistochemistry (IHC). In addition to conventional histology (H&amp;E), protein expression of e-cadherin, vimentin, Ki67 and α-smooth muscle actin (ASMA) was investigated by IHC. Results: Lower viability was seen in A549 monocultures compared to co-cultures, whereas Colo699 monocultures showed better viability compared to co-cultures. Ki67 expression varied significantly between mono- and co-cultures in both tumour cell lines. The co-cultivation with fibroblasts led to an increased expression of this protein indicating increased proliferation. Furthermore, a vimentin upregulation and an e-cadherin downregulation could be detected during the course of the cultivation indicating a transition to a more mesenchymal phenotype. Conclusion: We demonstrate that the hanging drops technology is a promising technique for the generation of tumour spheroid co-cultures. Furthermore, these spheroids allow the investigation of tumour-stroma interactions and a better reflection of in vivo conditions of cancer cells in their microenvironment. Our method holds potential to contribute to the development of anti-cancer agents and support the search for predictive biomarkers. Citation Format: Arno Amann, Marit Zwierzina, Gabriele Gamerith, Mario Bitsche, Julia M. Huber, Georg Vogel, Michael Blumer, Stefan Koeck, Jens Kelm, Wolfgang Hilbe, Heinz Zwierzina. Development of an innovative 3D cell culture system to study tumor-stroma interactions in cancer cells. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3840. doi:10.1158/1538-7445.AM2013-3840
Confocal laser endomicroscopy for the in vivo detection of intraepithelial neoplasia in Peutz–Jeghers polyps
Authors: H. Neumann, M. Vieth, M. Raithel, J. Mudter, R. Kiesslich, M. Neurath
Journal: Endoscopy (2010)

Peutz–Jeghers syndrome (PJS) is a rare, autosomal dominant condition that is characterized by hamartomatous polyps in the gastrointestinal tract and mucocutaneous melanin pigmentation [1] [2]. Confocal laser endomicroscopy (CLE) is rapidly emerging as a valuable tool for gastrointestinal endoscopic imaging, enabling the endoscopist to obtain an “optical biopsy” of the gastrointestinal mucosa during the endoscopic procedure [3] [4] [5].
Commentary: Ethics in conduct of trials in developing countries
Authors: C. Weijer
Journal: BMJ (2010)

The paper by Roth and colleagues1 on a randomised controlled trial of BCG revaccination in Guinea-Bissau highlights the importance of conducting vaccine trials in developing countries as well as the ethical difficulties faced by investigators in these settings. Guinea-Bissau has an infant mortality rate of 117 per 1000 live births, the fifth highest rate in the world.2 The best hope for substantial improvements in child health comes from public health interventions, including effective vaccine programmes. Vaccine efficacy might be lower in developing countries because of poverty and malnutrition. Once the efficacy of a vaccine is established, however, it is commonly assumed that it will confer a proportional survival benefit. The results of the BCG revaccination trial show that non-vaccine factors might undermine this assumption, and the authors conclude correctly that trials should examine the impact …
Quality of life in elderly subjects with a diagnostic label of asthma from general practice registers
Authors: C.A.E. Dyer, S.l Hill, R.a Stockley, A.J. Sinclair
Journal: European Respiratory Journal (1999)

The aim of this study was to assess health-related quality of life (QoL) in elderly subjects with a diagnostic label of asthma from a general practice population, and to determine the main contributory factors. Sixty people aged &gt; or =70 yrs with a primary care diagnostic label of asthma, and 43 control subjects were recruited. Assessment of bronchodilator response, and oral steroid trials were conducted where possible. The main outcome measures were QoL scores for the Short Form (SF)-36 and the St George9s Respiratory Questionnaire (SGRQ). In the asthma group, 29 subjects demonstrated a significant airway response to bronchodilators or steroids. Mean SF-36 scores were significantly worse in the total asthma group for components of physical function, physical role limitation, and general health, although psychological scores were similar. QoL remained worse than controls in those subjects with a significant bronchodilator response. Dyspnoea and depression accounted for 61% of the variance in the SGRQ, but forced expiratory volume in one second was not an independent variable. Quality of life is impaired in elderly people with a diagnosis of asthma, including those with demonstrable airway variability. Many older subjects with asthma note a variety of symptoms, highlighting the need for further research into the adequacy and efficacy of their treatment.
The Physician's Role in Areawide Planning
Authors: James H. Cavanaugh
Journal: JAMA (1966)

The majority of our population now lives in metropolitan areas, and the hospital and health problems of these areas constitute the problems of physicians, local and state government, national government, and society generally. The ability of physicians to cope with future hospital and related health facility planning problems will depend upon their ability to work towards effective solutions to the many hospital and health planning problems facing the nation today. Is it possible to overcome unrelated, individual, and sometimes selfish action on the part of some physicians and some hospital boards of trustees? Is it possible to attain overall hospital planning at the metropolitan and regional level? Is it possible to develop within the medical profession a vigorous and dedicated leadership that will let those persons responsible for patient care help our cities and metropolitan areas control their destinies? Many believe that it is possible, but that it will require
Materialism and Spiritualism: the Dualistic Way of Western Thinking
Authors: Johanna Margaretha Tamm
Journal: Psychotherapy and Psychosomatics (1979)

Ever since the beginning of Western philosophy in ancient Greece there have been two fundamental approaches to life in general: materialism and spiritualism. In the Renaissance this basic question came into discussion again. In the 19th century the real fight began. On the one hand we have had the materialistic 'leftists', namely Marx, with the political consequence of the Russian revolution. On the other hand we had Kierkegaard, Bergson, etc. searching for the fundamental facts in human life, enabling us to survive in dignity. The moment has come, where we must see that human emotion is by far more important in the question of life and death, namely in psychosomatic illness, than all the riches of the world.
Therapy of B-cell malignancies by anti–HLA-DR humanized monoclonal antibody, IMMU-114, is mediated through hyperactivation of ERK and JNK MAP kinase signaling pathways
Authors: Rhona Stein, Pankaj Gupta, Xiaochuan Chen, Thomas M. Cardillo, Richard R. Furman, Susan Chen, Chien-Hsing Chang, David M. Goldenberg
Journal: Blood (2010)
Clinical Tests of Oesophageal Function
Authors: D. A. W. Edwards
Journal: Gut (1976)

Six patients had isolated hemiataxia and ipsilateral sensory loss, as a manifestation of thalamic infarction in the thalamogeniculate territory. Acute hemiataxia-hypesthesia was not found in 1075 other patients from the Lausanne Stroke Registry who were admitted during the same period. Stroke onset was progressive in five patients and immediately complete in one. Five patients had an objective sensory loss. In two patients this affected light touch, pain and temperature sense, and in another three light touch, pain temperature, position and vibration sense. One patient had a purely subjective sensory disturbance. The sensory deficit cleared or was clearing although the ataxia persisted in all patients. On lesion mapping on CT or MRI, all patients had involvement of the lateral part of the thalamus (ventral posterior nucleus and ventral lateral nucleus). The presumed causes of stroke were cardioembolism in one patient, posterior cerebral artery occlusion in one patient and meningovascular syphilis in one patient, hypertensive small vessel disease in two patients, and undetermined in one patient. Hemiataxia-hypesthesia is a new stroke syndrome involving the perforating branches to the lateral thalamus, but in which small vessel disease may not be the leading cause.
P237 A Review Of The Steroid Sparing Impact Of Mycophenolate Mofetil In The Severe Asthma Population At The North West Lung Centre, University Hospital South Manchester
Authors: K. George, H. Brice, S. Fowler, L. Holmes, R. Daly, R. Niven
Journal: Thorax (2014)

<h3>Objectives</h3> To determine whether Mycophenolate mofetil (MMF) is an effective steroid sparing agent in a single centre cohort of severe asthma patients. <h3>Background</h3> MMF is a powerful inhibitor of purine and pyrimidine synthesis via the <i>de novo</i> pathway, upon which lymphocyte production is dependent. It is currently licensed for use in transplant rejection prophylaxis. Other immunosuppressant therapies have been used off licence in difficult-to-treat asthma patients under specialist supervision in an effort to reduce corticosteroid use. MMF is currently a third line immunosuppressant after methotrexate and azathioprine at the North West Lung Centre. <h3>Methods</h3> A retrospective data analysis was performed including all patients under specialist asthma care at UHSM that were previously or currently treated with MMF. Annualised average daily steroid dose was calculated from the available data in patient case notes. This was calculated for 12 months prior to commencing any immunosuppressant therapy and during MMF treatment. Exacerbation and hospital admission rates were also recorded. <h3>Results</h3> A total of 34 patients were identified as being on MMF for severe asthma for at least 8 weeks. 11 did not tolerate MMF or had no response and subsequently stopped. The primary analysis was carried out on 23 patients and a secondary post hoc analysis was performed on all patients who had been on treatment for a minimumof 6 months at the time of the study (N=12). The average yearly steroid sparing impact of MMF was 5.9 mg per day, (p &lt; 0.005). 74% had an overall reduction and 35% achieved a reduction of 10 mg or more. This value was lower in those who had been on treatment for &gt;6 months (Δ 3.9 p = 0.20). There was no statistically significant reduction in admission or exacerbation rates. <h3>Conclusion</h3> MMF has shown a small steroid sparing effect in this retrospective analysis, although the effect appeared less positive in the sub-group of those analysed after being on treatment for at least 6 months further analysis of the potential benefits of MMF in this patient population is required.
ELECTRON MICROSCOPIC OBSERVATIONS ON SKELETAL MUSCLE IN DIFFUSE SCLERODERMA.
Authors: R. MICHALOWSKI, J. KUDEJKO
Journal: British Journal of Dermatology (1966)

Journal Article ELECTRON MICROSCOPIC OBSERVATIONS ON SKELETAL MUSCLE IN DIFFUSE SCLERODERMA. Get access R. MICHALOWSKI, M.D., M.Ph., R. MICHALOWSKI, M.D., M.Ph. Dermatological Clinic, Dymitrowa 11, Lublin, Poland. Head: Ass. Prof. R. Michalowski, M.D., M.Ph Search for other works by this author on: Oxford Academic Google Scholar J. KUDEJKO, M.D. J. KUDEJKO, M.D. Laboratory of Electron Microscopy, Medical School, Lublin. Head: Prof. S. Grzycki, M.D Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 78, Issue 1, 1 January 1966, Pages 24–28, https://doi.org/10.1111/j.1365-2133.1966.tb12129.x Published: 01 January 1966
TCT-670 Impact of Intravascular Ultrasound Tissue Characterization, in Addition to Plaque Burden and Local Endothelial Shear Stress, on the Prediction of New Adverse Cardiac Events in Humans
Authors: Shingo Mizuno, Michail I. Papafaklis, Saeko Takahashi, Masaya Tsuda, Antonios Antoniadis, Ahmit U. Coskun, Shigeru Saito, Charles Feldman, Peter Stone
Journal: Journal of the American College of Cardiology (2013)
Effects on the Canine P Wave of Discrete Lesions in the Specialized Atrial Tracts
Authors: Albert L. Waldo, Harry L. Bush, Henry Gelband, George L. Zorn, Kari J. Vitikainen, Brian F. Hoffman
Journal: Circulation Research (1971)

The role of the specialized atrial tracts in determining the polarity, morphology, and duration of normal and ectopic P waves in the canine heart was studied by producing discrete surgical lesions in selected portions of these tracts. Effects of these lesions were correlated with changes in the polarity, morphology, and duration of the P waves and with conduction time to selected atrial sites when the atria were paced from the sinoatrial (SA) node, the left atrial portion of Bachmann's bundle, the low interatrial septum near the atrioventricular (AV) node, and the posterior-inferior left atrium. A lesion in the anterior internodal tract where it leaves the head of the SA node prolonged conduction time between the SA and AV nodes and significantly increased P wave duration when the atria were paced from the SA node and low interatrial septum sites. A lesion in the branch of the anterior internodal tract running through Bachmann's bundle significantly changed P wave polarity, morphology, and duration when the atria were paced from the SA node, Bachmann's bundle, and low interatrial septum sites. A lesion in the posterior internodal tract failed to change P wave polarity, morphology, or duration. When the atria were paced from the posterior-inferior left atrium, the lesions had no significant effect on P-wave polarity, morphology, or duration. It is concluded that specialized atrial tracts play a functionally important role in the sequence of atrial activation during many normal and ectopic rhythms.
Menstruation and Systemic Disease
Authors: Joseph Rogers
Journal: New England Journal of Medicine (1958)

Thyroid DisordersDisorders of thyroid function are frequently associated with disturbances of menstruation. In thyrotoxicosis, if a change in the menstrual cycle does occur, it almost invariably consists of oligomenorrhea or amenorrhea.119 In the series of Benson and Dailey,120 130 of 221 patients with diffuse toxic goiter had oligomenorrhea or amenorrhea. Ten patients experienced an increase in flow, and 81 had no change in menstrual pattern. It was of interest that amenorrhea seemed to have a relation to exophthalmos. In 18 of 31 patients with hypothyroidism that followed treatment of thyrotoxicosis, menorrhagia or polymenorrhea was present. It is generally agreed . . .
Extra-auricular Mitral Valvotomy
Authors: H. Baden, F. Therkelsen
Journal: Thorax (1954)

<h3>Abstract</h3> Timely and accurate knowledge of Dengue incidence is of value to public health professionals because it helps to enable the precise communication of risk, improved allocation of resources to potential interventions, and improved planning for the provision of clinical care of severe cases. Therefore, many national public health organizations make local Dengue incidence data publicly available for individuals and organizations to use to manage current risk. The availability of these data has also resulted in active research into the forecasting of Dengue incidence as a way to increase the public health value of incidence data. Here, we robustly assess time-series-based forecasting approaches against a null model (historical average incidence) for the forecasting of incidence up to four months ahead. We used publicly available data from multiple countries: Brazil, Mexico, Singapore, Sri Lanka, and Thailand; and found that our time series methods are more accurate than the null model across all populations, especially for 1-and 2-month ahead forecasts. We tested whether the inclusion of climatic data improved forecast accuracy and found only modest, if any improvements. We also tested whether national timeseries forecasts are more accurate if made from aggregate sub-national forecasts, and found mixed results. We used our forecasting results to illustrate the high value of increased reporting speed. This framework and test data are available as an R package. The non-mechanistic approaches described here motivates further research into the use of disease-dynamic models to increase the accuracy of medium-term Dengue forecasting across multiple populations. <h3>Author summary</h3> Dengue is a mosquito-borne disease caused by the Dengue virus. Since the Second World War it has evolved into a global problem, securing a foothold in more than 100 countries. Each year, hundreds of millions of people become infected, and upwards of 10,000 die from the disease. Thus, being able to accurately forecast the number of cases likely to emerge in particular locations is vital for public health professionals to be able to develop appropriate plans. In this study, we have refined a technique that allows us to forecast the number of cases of Dengue in a particular location, up to four months in advance. We test the approach using state-level and national-level data from Brazil, Mexico, Singapore, Sri Lanka, and Thailand. We found that the model can generally make useful forecasts, particularly on a two-month horizon. We tested whether information about climatic conditions improved the forecast, and found only modest improvements to the forecast. Our results highlight the need for both timely and accurate reports. We also anticipate that this approach may be more generally useful to the scientific community; thus, we are releasing a framework, which will allow interested parties to replicate our work, as well as apply it to other sources of Dengue data, as well as other infectious diseases in general.
Postoperative Evaluation of Myocardial Blood Flow in Aorta-to-Coronary Artery Vein Bypass Grafts Using the Xenon-Residue Detection Technic
Authors: P. LICHTLEN, T. MOCCETTI, J. HALTER, M. SCHÖNBECK, A. SENNING
Journal: Circulation (1972)

By the use of the xenon-residue detection technic, myocardial blood flow (MBF) was assessed 3-17 months after surgery in nine patients with an aorta-to-left anterior descending bypass (LAD-BP), in nine patients with an aorta-to-right coronary artery bypass (RCA-BP), and in two patients with both LAD-and RCA-BP. In addition, MBF was registered in the corresponding main coronary artery. In each case coronary angiography revealed a patent vein graft. LAD-BP flow averaged 58 ml/min/100 g, this being slightly lower than the average flow value recorded after injection into the main left coronary artery of these patients (64 ml/min/100 g). LAD-BP flow did not differ from the value recorded in 20 normal subjects (63.9 ml/min/100 g). In contrast, average RCA-BP flow amounted to 47.1 ml/min/100 g and was significantly higher than the values recorded in the obstructed main RCA of these patients (22.1 ml/min/100 g) ( P &lt; 0.0025) or those observed in 20 patients with severe disease of the right coronary artery (34.9 ml/min/100 g) ( P &lt; 0.025); no difference was seen between RCA-BP flow and the average of 20 normal subjects (46.1 ml/min/100 g). In six patients a two-to threefold increase of BP flow was found during exercise (50-100 w). Total coronary vascular resistance showed a similar behavior to MBF, not differing in the bypass area from the values obtained in the normal control groups. It is concluded that the venous bypass procedure substantially increases perfusion at rest and during exercise and that a good correlation exists between MBF and postoperative angiography.
Cell surface characterization of T lymphocytes and allergen-specific T cell clones: Correlation of CD26 expression with T H1 subsets
Authors: Martin Willheim, Christof Ebner, Karin Baier, Wolfgang Kern, Karl Schrattbauer, Ralf Thein, Dietrich Kraft, Heimo Breiteneder, Walter Reinisch, Otto Scheiner
Journal: Journal of Allergy and Clinical Immunology (1997)
Hemodynamic effects of the somatostatin analog lanreotide in humans: Placebo-controlled, cross-over dose-ranging echo-doppler study
Authors: Christian Mottet, Cornel C. Sieber, André Nauer, Jürgen Drewe, Ronald Fried, Finn Larsen, Christoph Beglinger
Journal: Hepatology (1998)

Because of their vasoactive effects, somatostatin and its analogs are increasingly used in the management of complications of chronic liver diseases such as variceal bleeding. Postprandial hyperemia augments splanchnic blood flow, subsequently increasing portal pressure. The aim of this study was to explore effects of the somatostatin analog, lanreotide, on food-stimulated hemodynamic parameters in healthy human subjects. A dose-response curve was constructed in eight healthy male subjects in a placebo-controlled cross-over study. On 4 different days, either 0 (placebo), 50, 100, or 200 μg/h of lanreotide was infused intravenously in random order, starting at 45 minutes for 7 hours. On each day, a liquid test meal (Ensure plus, 1.5 kcal/mL) was perfused intraduodenally at a rate of 3 mL/min over 7 hours after a 45-minute basal period. Diastolic arterial pressure (dBP), heart rate (HR), superior mesenteric arterial (SMA) average flow velocity (SMA-V), SMA pulsatility index (SMA-PI), portal venous volume flow (PV-F), and renal artery (RA) resistance index (RA-RI) were measured on regular intervals (flows using Echo-Doppler technology). Lanreotide at all doses abolished food-stimulated splanchnic hyperemia both in the SMA and PV over 7 hours. The fall in dBP and increase in HR after food perfusion were blunted under lanreotide. Food as well as lanreotide did not modify RA-RI. In summary: 1) lanreotide inhibits food-induced splanchnic hyperemia in normal subjects; 2) in parallel, systemic hemodynamic alterations to food stimulation are abolished with lanreotide; and 3) renal vascular resistance is unchanged. Because of its persistent splanchnic vasoconstrictive effect, lanreotide should be tested in patients with portal hypertension.
Prediction of severe coronary artery disease with Tc-99m sestamibi perfusion and function studies: A comparison with clinical history, physical examination, and electrocardiographic data
Authors: Salvador Borges-Neto, Leslee J. Shaw, Karen L. Kesler, Michael W. Hanson, Eric D. Peterson, Robert H. Jones, Edward Morris, R.Edward Coleman
Journal: Journal of the American College of Cardiology (1996)