tbuckley/cabot-search huggingface dataset:
Abstract 4364: Posttranscriptional regulation of tetraspanins CD151 &amp; CD9 in breast &amp; prostate cancers
Authors: Danielle R. Bond, Crystal Passfield, Murray Cairns, Leonie K. Ashman, Judith Weidenhofer
Journal: Cancer Research (2014)

Abstract Rationale The tetraspanins CD151 &amp; CD9 are known to play a prominent role in cancer progression by modulating cell adhesion and migration/invasion. CD151 is commonly overexpressed (metastasis enhancer) and tends to predict poor prognosis in many solid malignancies, whereas the metastasis suppressor CD9 is typically down-regulated which correlates with poor patient outcomes. However, progress in developing CD151 and CD9 as prognostic biomarkers &amp; therapeutic targets has been hampered by a lack of understanding of how they are regulated in normal cells and how they are deregulated in cancers. Objective Many micro-RNAs are deregulated in prostate and breast cancers. Bio-informatic analysis has identified 250 miRNAs that potentially regulate CD151 or CD9. Therefore, we investigated whether miRNAs regulate CD151 and CD9 in a range of normal and cancerous breast and prostate cell lines in vitro. Methods &amp; Results A dual luciferase reporter assay using the 3′UTR of CD151 and CD9 provided evidence that breast &amp; prostate cancer cell lines endogenously express miRNA that regulate tetraspanins and that the extent of regulation varies across the cell lines. Analysis of miRNA expression in normal and cancer cell lines using miRNA arrays and qPCR has confirmed that some miRNAs which are predicted to target CD9, are up-regulated in many prostate and/or breast cancer cell lines compared to non-tumourigenic cells. Furthermore, miRNAs were identified with altered expression that were predicted to target CD151. These miRNA have also been shown to directly regulate CD151 or CD9 using the dual luciferase 3′UTR reporter assay with miRNA mimics. Conclusion Altered miRNA expression may contribute to prostate and breast cancers by manipulating tetraspanins to allow cancer progression and metastasis. Therefore, miRNA may be effective prognostic and/or therapeutic targets for prostate and breast cancers in the future. Citation Format: Danielle R. Bond, Crystal Passfield, Murray Cairns, Leonie K. Ashman, Judith Weidenhofer. Posttranscriptional regulation of tetraspanins CD151 &amp; CD9 in breast &amp; prostate cancers. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4364. doi:10.1158/1538-7445.AM2014-4364
Abstract 20164: Profiling of Circulating MicroRNAs After Heart Transplantation and During Acute Cellular and Antibody-Mediated Rejection
Authors: Raymond Givens, Danielle Templeton, Ruiping Ji, Peter Kennel, Donna Mancini, Yoshifumi Naka, Hiroo Takayama, Isaac George, P. C Schulze
Journal: Circulation (2014)

Introduction:  Heart transplantation (HTx) is the definitive treatment for advanced heart failure. Despite universal immunosuppressive therapy, the threat of allograft rejection is persistent. Rejection is generally diagnosed by histology of graft myocardium obtained by biopsy which is invasive and prone to sampling error. Study of novel biomarkers such as circulating microRNAs (miRs) may allow noninvasive diagnosis of rejection.   Methods:  We studied serum levels of miRs associated with immune cell function (miR-155, 125b, 142-3p, 144, 223-3p, 27a and 101), myocardial damage (miR-1) and immunosuppression (let-7a). Blood was obtained from HTx recipients >2 months post-HTx. Rejection was defined by histology of cardiac biopsy specimens and graded according to ISHLT guidelines. MiRs were analyzed with RT-PCR and normalized to 5S rRNA.   Results:  We analyzed samples from 12 healthy volunteers (44±6 yrs), 12 HTx recipients without evidence of rejection (54±11 yrs), 11 patients with acute cellular rejection (ACR; 48±12 yrs), and 6 patients with antibody-mediated rejection (AMR; defined by C4D+ immunohistochemistry; 48±13 yrs). The ACR group included 7 patients with ISHLT 1R/1B rejection and 4 with 2R/3A. 6 of 11 ACR and 5 of 6 AMR patients had evidence of donor-specific antibodies. Let-7a levels were significantly lower among the HTx group vs. controls (0.09±0.13 vs. 1.00±1.53 RU; p=0.02) and increased only among the ACR group (0.44±0.64, p=0.03) vs. the HTx group. MiR-223-3p was lower among HTx (0.31±0.38), ACR (0.33±0.37) and AMR (0.38±0.37) vs. control (1.00±0.87, p   Conclusion:  Circulating miRNAs related to immune function may aid in diagnosis of cardiac allograft rejection. Our data show that miR-101 and 223-3p and let-7a have diagnostic potential for identifying patients with ACR and AMR. Our ongoing studies will analyze roles for miRs as ancillary markers of graft rejection following HTx.
Inflammation in traffic professional from Sao Paulo after pollutants emission control implementation
Authors: Maria Lucia Bueno Garcia, Lourdes Conceição Martins, Alfesio Braga, Miriam Lemos, Luis Pereira, Paulo Afonso, Paulo Saldiva, Paula Santos Ubiratan
Journal: European Respiratory Journal (2012)

In the last decade, ambient particles have decreased from 150ug/m3 to 40ug/m3 in Sao Paulo city (SP) because of public policies to control fuel emissions Traffic professional are more exposed to air pollution. We tested if ambient air from SP is still deleterious to traffic professionals. Non or ex-smokers (≥1 year) cab drivers (N=46) and traffic controllers (N=23) were evaluated 4 times. We checked clinical symptoms and blood inflammatory markers (HDL/LDL/total cholesterol, triglycerides, blood cell counting, clot tests, ultra sensitive c reactive protein- us CRP and erythrocyte sedimentation rate-ESR) on the day after workshift. Pollutants were collected during 24h exposure by individual samplers for fine particles (reflectance) and NO2(colorimetry). Clinical and blood data were tested against pollutants by linear regression model for repeated measures through generalized estimated equation (GEE). Alpha was 5%. PM2.5 was 40,33±20.83ug/m3 and NO2 197±43,47ugm3. Traffic professional referred cough (19%), coryza (21,4%), sneezing (35,6%), nasal stuffiness (34,5%)and itch(28,6%), rhinitis (35,6%), wheezing (14,3%), dyspnea (10,7%) which improved during non working periods (60,7%)(holidays or weekends). ESR, clot tests (prothrombin INR, activated partial thromboplastin time and thromboplastin time), LDL chol and mononuclear cells in blood were associated to both PM2.5 (p
Abstract 3975: PID1, a new growth-inhibitory gene, sensitizes brain tumor cell lines to chemotherapy
Authors: Jingying Xu, Xiuhai Ren, Anthony Tran, Gregory M. Shackleford, Anat Erdreich-Epstein
Journal: Cancer Research (2014)

Abstract BACKGROUND: Phosphotyrosine Interaction Domain containing protein-1 (PID1) is a gene discovered in 2006 in the context of obesity, which we recently showed inhibits growth of brain tumor cell lines and its mRNA level is directly correlated with survival of patients with gliomas and medulloblastomas (Erdreich-Epstein et al, Clin Cancer Res, In Press). PID1 mRNA was lower in more aggressive medulloblastomas and gliomas, and patients whose tumor had higher PID1 mRNA had longer survival. Moreover, overexpression of PID1 markedly decreased 2D colony formation in cell lines of medulloblastomas, GBM and atypical teratoid rhabdoid tumors (ATRT). The growth-inhibitory effect was due to both decreased proliferation and increased cell death. METHODS/RESULTS: COSMIC showed no mutations of PID1 in brain tumors, suggesting that PID1 was not a formal tumor suppressor gene. Therefore, we hypothesized that PID1 may sensitize brain tumor cells to therapy, and thus account for the longer survival in patients with higher PID1 mRNA. Indeed, while both cisplatin (10µg/ml) or transient PID1 overexpression increased apoptosis of glioma and medulloblastoma cell lines (indicated by increased AnnexinV and mitochondrial depolarization), combining cisplatin with PID1 caused a markedly higher apoptosis. Moreover, knockdown of PID1 by siRNA inhibited the cisplatin-induced mitochondrial membrane depolarization and apoptosis (AnnexinV), suggesting that PID1 may be required for cisplatin-induced apoptosis. Taken together, this suggests a sensitizing effect of PID1 to chemotherapy in both glioma and medulloblastoma cell lines. Intriguingly, PID1 mRNA and protein both increased in response to etoposide (5µg/ml), vincristine (50ng/ml) or cisplatin (5µg/ml) in a time- and dose-dependent manner in the brain tumor cell lines. This chemotherapy-induced increase in PID1 mRNA was blocked by inhibitors of NFKB, suggesting that regulation of PID1 may be an NFKB-dependent mechanism. Ongoing work seeks to decipher the mechanism of these effects and test them in vivo in mouse models. CONCLUSIONS: Our data suggest that PID1 acts to sensitize glioma and medulloblastoma cells to chemotherapy, possibly explaining the correlation between higher PID1 mRNA and survival in patients. Citation Format: Jingying Xu, Xiuhai Ren, Anthony Tran, Gregory M. Shackleford, Anat Erdreich-Epstein. PID1, a new growth-inhibitory gene, sensitizes brain tumor cell lines to chemotherapy. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3975. doi:10.1158/1538-7445.AM2014-3975
Contemporary Issues in Bioethics.
Authors: Tom L. Beauchamp, LeRoy Walters
Journal: Annals of Internal Medicine (1978)

The strength of this book on contemporary issues in bioethics is that it examines many of the critical medical-ethics issues that confront all of us today.Bioethics has become the business of everyone because it touches the lives of everyone.The ethical implications of issues like genetic engineering, genetic therapy, physician-assisted suicide, nanotechnology, reproductive technologies, stem cell research, cloning, endof-life issues, organ transplantation, and health care reform go beyond individual hospitals, research centers and nursing homes.How these issues are resolved will determine for years and even decades who we are, not only as individuals, but who we are as a global community.These issues need to be examined broadly by individuals of varying talents, because these issues are far too important and lifechanging to be left in the hands of a few medical professionals and researchers.The main strength of this book is that this international exchange of ideas will not only highlight many of these crucial issues but will strengthen the discipline of bioethics globally.A critical exchange of ideas allows everyone to learn and benefit from the insights gained through others' experiences.Analyzing and understanding real bioethics issues and cases and how they are resolved is the basis of education in bioethics for those who will have to make these decisions in the future.The more we examine, analyze, and debate these bioethics issues and cases, the more knowledge will be gained and hopefully, we will all gain more practical wisdom for the benefit of humankind.
Plasma angiotensin-converting enzyme activity and carotid wall thickening.
Authors: M N Payne, F McDonald, R G Murray, W A Bartlett, A F Jones, J M Beattie
Journal: Circulation (1994)

with echocardiographs, including ours; the frequency area was 20 to 600 Hz, but the sensitivity was optimal at higher frequencies (>100 Hz).Finally, in defense of Circulation's "eagle-eyed custo- dians," we would like to add that one of the reviewers commented, like Dr Spodick, that there should have been two, preferably three, independent auscultators in our study.We admit that this would have improved our work, but we have no reason to believe that the results would have been different.
Common Diagnostic Tests: Use and Interpretation.
Authors: Harold C Sox
Journal: Annals of Internal Medicine (1991)

The second edition of this guide contains revised and updated chapters from the first edition and completely new chapters on diagnostic reasoning, thyroid function tests, and glycemia tests. Among the tests covered are routine chest radiographs, blood cultures, carcinoembryonic antigen tests, and throat cultures and rapid tests for diagnosis of A streptococcal pharyngitis.
Non-Destructive Analysis of the Nuclei of Transgenic Living Cells Using Laser Tweezers and Near-Infrared Raman Spectroscopic Technique
Authors: Wei Tang, Ronald J. Newton, Chang-An Xie, Yong-Qing Li, Nicki Whitley
Journal: Genomics Proteomics & Bioinformatics (2005)

Transgenic cell lines of loblolly pine (Pinus taeda L.) were analyzed by a compact laser-tweezers-Raman-spectroscopy (LTRS) system in this investigation. A low power diode laser at 785 nm was used for both laser optical trapping of single transgenic cells and excitation for near-infrared Raman spectroscopy of the nuclei of synchronized cells, which were treated as single organic particles, at the S-phase of the cell cycle. Transgenic living cells with gfp and uidA genes were used as biological samples to test this LTRS technique. As expected, different Raman spectra were observed from the tested biological samples. This technique provides a high sensitivity and enables real-time spectroscopic measurements of transgenic cell lines. It could be a valuable tool for the study of the fundamental cell and molecular biological process by trapping single nucleus and by providing a wealth of molecular information about the nuclei of cells.
A Novel Prostate-Specific Adenovirus for Gene Expression in Hormone-Sensitive and Hormone-Refractory Prostate Cancer Cells
Authors: Wing-Shing Cheng, Magnus Essand
Journal: Molecular Therapy (2004)

To develop a transcriptional regulatory sequence for prostate-specific transgene expression that is more potent then the ones currently used in experimental and clinical prostate cancer gene therapy, we constructed various recombinant expression control sequences comprising the TARP promoter (TARPp) and the PSA enhancer (PSAe) and/or the PSMA enhancer (PSMAe). Transcriptional activities of the expression control sequences were examined by luciferase reporter gene assays on transiently transfected cells. We found that gene expression controlled by a chimeric regulatory sequences comprising PSAe linked to PSMAe, in turn linked to TARPp (PPT) is highly prostate-specific and active in prostate cancer cells both in the presence and absence of testosterone. Since prostate cancer patients are often treated by androgen withdrawal, it may be beneficial to the patient to use a regulatory sequence that results in high transgene expression also in the absence of testosterone. Recombinant adenoviruses with the PPT sequence in the E1 position of an E1/E3-deleted adenovirus serotype 5 vectors were constructed. A DNA insulator from the mouse H19 imprinting control region was inserted directly upstream of the expression cassette to protect the transcriptional integrity of PPT from interfering adenoviral sequences. The produced viruses confer prostate-specific transgene expression when screened on cell lines of various origins. We believe that an adenovirus with E1A and/or other therapeutic gene expression, controlled by the H19 insulator-shielded PPT regulatory sequence, is a promising candidate for prostate cancer gene therapy.
Stroke: Pathophysiology, Diagnosis, and Management.
Authors: H. J. M. Barnett
Journal: Annals of Internal Medicine (1988)

Section I: Pathophysiology of Stroke. Epidemiology of Stroke. Atherosclerosis. Vascular Biology of Cerebral Arteries. Neurochemistry and Molecular Biology. Pathophysiology of White Matter Anoxic/Ischemic Injury. Positron Emission Tomography. Functional Magnetic Resonance Imaging. Pathology. Basic Science of Fibrinolysis: Coronary and Cerebral Thrombolysis. Section Ii: Diagnostic Studies for Stroke. Overview of Laboratory Studies. Computed Tomography Scanning. Magnetic Resonance Scanning. Cerebral Angiography. Ultrasound Imaging and Doppler Sonography. Single-Photon Emission Computed Tomography. Section Iii: Clinical Manifestations of Stroke. Classification of Ischemic Stroke. Internal Carotid Artery Disease. Anterior Cerebral Artery Disease. Middle Cerebral Artery Disease. Posterior Cerebral Artery Disease. Choroidal Artery Disease. Vertebrobasilar Occlusive Disease. Lacunes. Cerebral Venous Thrombosis. Intracerebral Hemorrhage. Intracranial Aneurysms. Brain Vascular Malformations. Spinal Cord Ischemia. Section Iv: Specific Medical Diseases and Stroke. Dissections and Trauma of Cervicocerebral Arteries. Stroke in the Setting of Collagen Vascular Disease. Moyamoya Disease. Cerebrovascular Fibromuscular Dysplasia. Migraine and Stroke. Hypertensive Encephalopathy. Multi-Infarct Dementia. Atherosclerotic Disease of the Aortic Arch. Binswanger's Disease. Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (Cadasil). Cerebral Amyloid Angiopathy. Coagulation Abnormalities in Stroke. Stroke and Substance Abuse. Stroke in Young Adults. Patent Foramen Ovale and Ischemic Stroke. Section V: Stroke Therapy. Principles of Hemostasis and Antithrombotic Therapy. Platelet Function and Antiplatelet Agents. Antithrombotic Therapy in Diseases of the Cerebral Vasculature. Pharmacologic Modification of Acute Cerebral Ischemia. Cardiogenic Brain Embolism: Incidence, Varieties, And Treatment. Medical Complications of Stroke. The Intensive Care of the Stroke Patient. Thrombolytic and Defibrino
SUBACUTE BACTERIAL ENDOCARDITIS OF THE MITRAL VALVE PREVIOUSLY RENDERED INCOMPETENT BY INFARCTION OF THE PAPILLARY MUSCLE AND SHORTENING OF THE CHORDAE TENDINAE
Authors: I. I. Lemann
Journal: Annals of Internal Medicine (1936)

Case Reports1 May 1936SUBACUTE BACTERIAL ENDOCARDITIS OF THE MITRAL VALVE PREVIOUSLY RENDERED INCOMPETENT BY INFARCTION OF THE PAPILLARY MUSCLE AND SHORTENING OF THE CHORDAE TENDINAEI. I. LEMANN, M.D., F.A.C.P.I. I. LEMANN, M.D., F.A.C.P.Author, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-9-11-1587 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe present communication is made as a contribution to the study of two problems: first, the production of murmurs in coronary infarction; and second, the pathogenesis of subacute bacterial endocarditis. The occurrence of murmurs and of subacute bacterial endocarditis in relation to coronary infarction have both been repeatedly reported. The significance which I wish to attribute to them in the light of the case to be reported is new.CASE REPORTMale, aged 53 (at the time of the attack), suffered a coronary infarction on January 2, 1931. The evidence was convincing. He had pain in his chest and numbness...References1. WHITE P: Heart disease, 1931, MacMillan Co., New York, p. 420 and p. 423. Google Scholar2. LEVINE S: Coronary thrombosis: its various clinical features, Medicine, 1929, viii, 266. Google Scholar3. CASTEX MR: Merosystolic souffles, Prenza méd. Argent., 1931, xviii, 781. Google Scholar4. CASTEX MR: Souffles of the heart, Prenza méd. Argent., 1932, xix, 1153. Google Scholar5. DICIOBATTRO AA: Value of the merosystolic souffle in the diagnosis of infarct of the myocardium, Prenza méd. Argent., 1933, xx, 150. Google Scholar6. HYMANPARSONNET ASAE: The failing heart of middle age, 1932, F. A. Davis Co., Philadelphia, p. 175. Google Scholar7. SMITH FM: Diseases of the heart, in MUSSER'S Internal Medicine (Chapter XI), 2 Ed., 1934, Lea and Febiger, Philadelphia, p. 377. Google Scholar8. TORRES CM: On thrombosis of heart and mural endocarditis of non-valvular origin, Mem. d. Instit. Oswaldo Cruz, 1928, xxi, 268. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: New Orleans, Louisiana*Read by title at the Meeting of the Association of American Physicians, May 1935.From the Department of Medicine, School of Medicine, Tulane University, and The Medical Service, Touro Infirmary. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited bySubacute Bacterial Endocarditis Arising in Mural Thrombi Following a Myocardial Infarction: A Case ReportNichtrheumatische Endokarditis 1 May 1936Volume 9, Issue 11Page: 1587-1595KeywordsBacterial pathogensEndocarditisInfarctionMedical servicesPapillary musclesPathogenesisThorax ePublished: 1 December 2008 Issue Published: 1 May 1936 PDF downloadLoading ...
Tattoo inks in general usage contain nanoparticles
Authors: T. Høgsberg, K. Loeschner, D. Löf, J. Serup
Journal: British Journal of Dermatology (2011)

To our knowledge tattooing has never been thought of as a method of introducing nanoparticles (NPs) into the human body by the intradermal route, and as such it has never been a topic of research in nanotoxicology. The content of NPs in tattoo inks is unknown.To classify the particle sizes in tattoo inks in general usage.The particle size was measured by laser diffraction, electron microscopy and X-ray diffraction.The size of the pigments could be divided into three main classes. The black pigments were the smallest, the white pigments the largest and the coloured pigments had a size in between the two. The vast majority of the tested tattoo inks contained significant amounts of NPs except for the white pigments. The black pigments were almost pure NPs, i.e. particles with at least one dimension <100 nm.The finding of NPs in tattoo inks in general usage is new and may contribute to the understanding of tattoo ink kinetics. How the body responds to NP tattoo pigments should be examined further.
Case History: Persephone
Authors: Peter Pereira
Journal: JAMA (2003)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Quality of Cardiopulmonary Resuscitation—Reply
Authors: Lars Wik
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal