tbuckley/cabot-search huggingface dataset:
RELATION OF MYOCARDIAL STRAIN WITH LEFT VENTRICULAR GEOMETRY AFTER ACUTE MYOCARDIAL INFARCTION
Authors: Maria Louisa Antoni, Victoria Delgado, Jael Z. Atary, Eduard R. Holman, Ernst E. Van der Wall, Martin J. Schalij, Jeroen J. Bax
Journal: Journal of the American College of Cardiology (2010)
Comparison of different miR-21 inhibitor chemistries in a cardiac disease model
Authors: Thomas Thum, Nelson Chau, Balkrishen Bhat, Shashi Kumar Gupta, Peter S. Linsley, Johann Bauersachs, Stefan Engelhardt
Journal: Journal of Clinical Investigation (2011)

We would like to comment on a recent study that analyzed the role of microRNA-21 (miR-21) in a mouse model of cardiac disease (1). Using miR-21–deficient mice and novel, very short, 8-nucleotide anti–miR-21 oligonucleotides, the authors failed to detect any modulation of pressure overload-induced myocardial hypertrophy and fibrosis and concluded that miR-21 plays no role in cardiac disease. In contrast, we and others reported that inhibition of miR-21 with highly specific, 22- and 15-nucleotide-long anti–miR-21 oligonucleotides effectively inhibits myocardial and pulmonary fibrosis (2, 3). While genetic deletion of a target may lead to compensation during development and is often different from pharmacological inhibition of this target in the adult organism, the discrepancy between the therapeutic trials using long versus short 8-mer oligonucleo­tides is striking. We therefore carried out a direct head-to-head comparison of three different oligonucleotide chemistries (Figure ​(Figure1A)1A) in the same model of pressure overload-induced cardiac hypertrophy (transaortic constriction [TAC]). The two 22-mer oligonucleotides were complementary to the full-length miR-21, while the 8-mer was complementary to nucleotides 2 to 9 of miR-21, locked nucleic acid modified (LNA modified), and identical to the oligonucleotide used in the report by Patrick et al. (Figure ​(Figure1A). 1A). 



Figure 1

Comparison of different miR-21 oligonucleotide inhibitors.
Herpes viruses induce atherosclerosis in chickens
Authors: Elizabeth Rasche González
Journal: JAMA (1980)

Domestic birds who have not run afoul of the Colonel may face an even harder fate at the hands of New York Hospital-Cornell University Medical Center investigators. Determined to learn more about human coronary disease by studying fowl, these researchers have recently induced atheromatous plaque formation in specific-pathogen-free White Leghorns by inoculating them intratracheally with Marek's disease herpes virus (MDV). C. Richard Minick, MD, professor of pathology at Cornell, discussed the group's findings (<i>Am J Pathol</i>96:673-706, 1979) at the recent American Heart Association science writers' forum at St Petersburg Beach, Fla. Minick and associates divided more than 100 male chicks with normal cholesterol levels into four groups. Two of the groups were inoculated with MDV two days after birth; two were not. All groups were fed a low-cholesterol diet for 15 weeks. For 15 additional weeks, one infected group and one uninfected group received a 2% cholesterol supplement to
PM-11 * DEVELOPMENT OF A NOVEL GENE TARGETING AND CLONE SCREENING PLATFORM TO ENGINEER COMMON PEDIATRIC GLIOMA MUTATIONS INTO MODEL CELL LINES
Authors: N. Fons, R. Sundaram, R. Bindra
Journal: Neuro-Oncology (2015)

The utility of CRISPR/Cas9 gene targeting tools to alter endogenous genomic loci has revolutionized biomedical research across numerous disease focus areas, with especially large gains made in the development of disease models. However, the introduction of specific mutations using donor DNA templates can be associated with low yields of single cell clones harboring homozygous versus heterozygous mutations. Conventional clone screening methods can be a labor intensive and expensive process, and thus better approaches are needed to identify cells with the desired genotype(s). In parallel, there is a dearth of human tumor cell model systems for pediatric cancers. To address these needs, we recently designed and validated a platform which utilizes a novel, inducible Cas9 expression system coupled with a high-resolution DNA melt (HRM) analysis protocol to rapidly identify mutant clones. We applied our platform to model several common DIPG mutations, including the Lys27Met missense mutation within histone variant H3.3 (H3F3A), and truncating mutations within protein phosphatase 1D (PPM1D) exon 6. Using our HRM protocol, both heterozygous point mutations in H3F3A, and indels in PPM1D were detectable and grouped distinctly from wild-type samples. These findings were validated using conventional Sanger sequencing and topo cloning, which confirmed that this approach could differentiate between mutant and wild-type clones. Multiple clones were generated in under four weeks using this process-flow. These findings highlight the utility and power of our unique approach to rapidly create engineered mutant cell lines. Our platform likely will become an invaluable tool for the development of better pediatric glioma cell line models in the future.
Some Practical Aspects of the Smoking-Cancer Problem
Authors: Ernest L. Wynder, Dietrich Hoffmann
Journal: New England Journal of Medicine (1960)

THE majority of those who have investigated the epidemiology of lung cancer are in agreement that smoking, particularly of cigarettes, represents one of the causes of cancer of the lung. Several health authorities have publicly supported this view. These include the United States Surgeon General, Leroy Burney, the Director of the National Cancer Institute, John Heller, the American Cancer Society, the Health Commissioner of the State of New York, Herman Hilleboe, and the public-health services of Great Britain and the Netherlands.1 2 3 4 5 6 It is no longer an argument whether smoking affects the development of lung cancer, but rather a question of . . .
NEW METHODS FOR BLOOD TRANSFUSION AND SERUM THERAPY
Authors: FRANK W. HARTMAN
Journal: JAMA (1918)

<h3>1. A ONE MAN CLOT PROOF TRANSFUSION APPARATUS</h3> In working more or less constantly during the past year with blood transfusion by the citrate method, we have found numerous pitfalls and have tried to simplify the apparatus, eliminating chances of failure. The principal difficulty has been to use a needle small enough so that one trial is enough to get well into the vein and still prevent clotting long enough to get the desired amount of blood. A 17-gage needle (<i>g</i>), preferably platinum, is large enough and causes the donor no marked discomfort. However, as the blood reaches the tube leading to the container there is a slowing of the stream because of the large bore of the tube compared with that of the needle. The walls of this tubing soon become coated with blood cells and a clot filling the lumen results, often before the desired amount of blood
A COMPARATIVE STUDY OF ANTIHY ALURONIDASE, ANTISTREPTOLYSIN “O,” ANTISTREPTOKINASE, AND STREPTOCOCCAL AGGLUTINATION TITERS IN PATIENTS WITH RHEUMATIC FEVER, ACUTE HEMOLYTIC STREPTOCOCCAL INFECTIONS, RHEUMATOID ARTHRITIS AND NON-RHEUMATOID FORMS OF ARTHRITIS 1
Authors: Robert W. Quinn, Sung J. Liao
Journal: Journal of Clinical Investigation (1950)
Role of Diabetologist in Evaluating Diabetic Retinopathy
Authors: David M Nathan, Howard A Fogel, John E Godine, Peter L Lou, Donald J D'Amico, Charles DJ Regan, Trexler M Topping
Journal: Diabetes Care (1991)

objective To evaluate the ability of diabetologists to screen diabetic patients for diabetic retinopathy. Research Design and Methods Comparison of eye examination performed by diabetologists with direct ophthalmoscopy through an undilated pupil and by ophthalmologists through a dilated pupil with seven-field stereoscopic fundus photography (gold standard). The study consisted of 67 insulin-dependent and non-insulin-dependent diabetic outpatients attending a diabetes clinic. Results On the basis of fundus photography, patients were classified as having no or insignificant (30%), minimal (31%), moderate (24%), or severe (15%) retinopathy. The diabetologists and ophthalmologists performed similarly in their ability to classify severity of diabetic retinopathy accurately. When no or insignificant retinopathy (isolated microaneurysms only) was detected by examination, clinically significant retinopathy detected by fundus photography was highly unlikely (&amp;lt; 5%). On the other hand, if more than isolated microaneurysms were seen on examination, all examiners missed more severe lesions detected by fundus photography. Patients with corrected visual acuity worse than 20/30 had a high likelihood (100%) of moderate or severe retinopathy. Conclusions Motivated well-trained diabetologists can screen for diabetic retinopathy. The absence of detectable lesions by direct ophthalmoscopy indicates that automatic referral to an ophthalmologist is not necessary. However, if any level of retinopathy is detected or corrected acuity is worse than 20/30, referral to an ophthalmologist is required. In this setting, fundus photography is advised because it is the most sensitive means of detecting clinically significant retinopathy. If other nonophthalmologists can be trained to achieve similar results, current recommendations for ophthalmologic referral that require annual ophthalmologic examinations for most diabetic patients may need to be reconsidered.
Alternative providers of GP services perform worse than traditional practices
Authors: Z. Kmietowicz
Journal: BMJ (2015)

Researchers have warned that the quality of care in the NHS in England may suffer from the influx of private and voluntary sector providers, after finding that the performance of alternative providers of GP services was worse than that of traditional practices on a range of indicators.

The researchers, from Imperial College London, compared the quality of primary care services provided under new alternative provider contracts with that in traditional models of general practice. In the open cohort study they examined data on how all general practices in England operating between 2008-09 and 2012-13 performed on 17 established quality indicators covering clinical effectiveness, safety, patients’ experience, timeliness …
PON-dering differences in HDL function in coronary artery disease
Authors: Chieko Mineo, Philip W. Shaul
Journal: Journal of Clinical Investigation (2011)

HDL cholesterol activates endothelial cell production of the atheroprotective signaling molecule NO, and it promotes endothelial repair. In this issue of the JCI, Besler et al. provide new data indicating that HDL from stable coronary artery disease (CAD) or acute coronary syndrome patients inhibits rather than stimulates endothelial NO synthesis and endothelial repair. This may be related to decreased HDL-associated paraoxonase 1 (PON1) activity. These observations support the concept that the cardiovascular impact of HDL is not simply related to its abundance, and the translation of the present findings to prospective studies of CAD risk and to evaluations of HDL-targeted therapeutics is a logical future goal.
Esophageal Eosinophilic Infiltration Responds to Proton Pump Inhibition in Most Adults
Authors: Javier Molina–Infante, Lucia Ferrando–Lamana, Cristina Ripoll, Moises Hernandez–Alonso, Jose M. Mateos, Miguel Fernandez–Bermejo, Carmen Dueñas, Nuria Fernandez–Gonzalez, Eva M. Quintana, Maria Angeles Gonzalez–Nuñez
Journal: Clinical Gastroenterology and Hepatology (2010)
Relation of Diet to Caloric Expenditure
Authors: James O. Hill
Journal: Annals of Internal Medicine (1994)

Letters15 March 1994Relation of Diet to Caloric ExpenditureJames O. Hill, PhD, Holly Drougas, RD, and John C. Peters, PhDJames O. Hill, PhDUniversity of Colorado Health Sciences Center; Denver, CO 80262Vanderbilt University; Nashville, TN 37232The Procter & Gamble Company; Ross, OH 45601Search for more papers by this author, Holly Drougas, RDUniversity of Colorado Health Sciences Center; Denver, CO 80262Vanderbilt University; Nashville, TN 37232The Procter & Gamble Company; Ross, OH 45601Search for more papers by this author, and John C. Peters, PhDUniversity of Colorado Health Sciences Center; Denver, CO 80262Vanderbilt University; Nashville, TN 37232The Procter & Gamble Company; Ross, OH 45601Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-120-6-199403150-00017 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail IN RESPONSE:We have reviewed the letter about our article on diet composition. Dr. Citkowitz makes two points in this letter. The first is that we underestimated the importance of the thermic effect of food between a high- and low-fat diet. Unfortunately, Table 1 contained a typographical error. In column 1, the thermic effect of fat should be listed as 10 kcal rather than 90 kcal. Thus, Dr. Citkowitz's argument that the difference in the thermic effect of food between a high- and low-fat diet is substantial is not true. As we state in the text, this difference is small ... Author, Article, and Disclosure InformationAffiliations: University of Colorado Health Sciences Center; Denver, CO 80262Vanderbilt University; Nashville, TN 37232The Procter & Gamble Company; Ross, OH 45601 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 15 March 1994Volume 120, Issue 6Page: 522-523KeywordsBioenergeticsBody weightDietEatingExerciseFatsFoodWeight gain ePublished: 15 August 2000 Issue Published: 15 March 1994 Copyright & PermissionsCopyright © 1994 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Normal erythrocyte sedimentation rate in the elderly.
Authors: R A Griffiths, W R Good, N P Watson, H F O'Donnell, P J Fell, J M Shakespeare
Journal: BMJ (1984)

Two hundred subjects aged 60-89 were selected for a study aimed at defining a reference range for the erythrocyte sedimentation rate in the elderly. The study extended a previous survey in subjects aged 20-65. The results confirmed that the sedimentation rate increases with age and that women have higher values than men but suggested that over half of elderly patients with disease would have rates within the previously defined "normal" range. It is therefore suggested that an erythrocyte sedimentation rate exceeding 19 mm in the first hour in elderly men and 22 mm in the first hour in elderly women warrants investigation.
Judge overrules mothers' objections to MMR vaccine
Authors: C. Dyer
Journal: BMJ (2003)

A High Court judge in London last week ordered two girls aged 4 and 10 to be given the combined measles, mumps, and rubella (MMR) vaccination, overruling their mothers' objections.

Mr Justice Sumner said the court would not normally force parents to vaccinate their children. However, he said, in the two cases before him, brought by fathers who were separated from their daughters' mothers, the court had to decide what would be in the children's best interests because the parents were in dispute. None of the parties to the case can be identified.

The 4 year old's mother, …
Role of Ca2+ availability to myofilaments and their sensitivity to Ca2+ in myocyte contractile dysfunction in heart failure
Authors: S Kinugawa
Journal: Cardiovascular Research (1999)

Objective: Contractile function is depressed at the isolated myocyte level in heart failure (HF), which could result from the decreased availability of intracellular calcium ([Ca2+]i) to the myofibrils and/or the depressed sensitivity of myofilaments to [Ca2+]i. However, the cellular basis of contractile dysfunction remains unestablished. Methods: We isolated left ventricular myocytes from dogs with rapid pacing-induced HF. Cell shortening and [Ca2+]i transients were measured by indo-1 fluorescence and the myofilament Ca2+ sensitivity was analyzed by the shortening–[Ca2+]i relation in intact myocytes as well as by the pCa–tension relation in skinned cells. Results: Peak cell shortening magnitude was depressed in HF, associated with a parallel decrease of [Ca2+]i transient amplitude. There was a significant positive correlation between these two variables (r=0.71, P<0.01). In contrast, myofibrillar sensitivity to Ca2+, determined by both intact and skinned myocytes, was comparable between control and HF. Further, there was no significant difference in Ca2+ sensitivity between control and HF even at shorter (1.8 μm) or longer (2.2 μm) sarcomere length. Conclusions: Using both intact and skinned cellular preparations, a potential defect in myocyte contractile function in HF was a reduction in Ca2+ availability to the myofilaments, rather than the inherent defects in myofilament sensitivity to Ca2+.
Screening for abdominal aortic aneurysm
Authors: Paul E Norman, Konrad Jamrozik, Michael M Lawrence-Brown, Max T Q Le
Journal: BMJ (2005)

Editor—We reported the study in a transparent fashion and were deliberately cautious in our conclusions. Australia and the United Kingdom are very different with regard to arrangements for primary care, which did not permit us to undertake a preliminary assessment of the eligibility of men for screening before we randomised them and issued half invitations to attend for the ultrasound examination.
AG-221 offers a survival advantage in a primary human IDH2 mutant AML xenograft model
Authors: K. Yen, F. Wang, J. Travins, Y. Chen, H. Yang, K. Straley, S. Choe, M. Dorsch, S. Agresta, D. Schenkein, S. Biller, M. Su
Journal: Annals of Oncology (2015)
Proteomic profiling in schizophrenia: enabling stratification for more effective treatment
Authors: Paul C Guest, Daniel Martins-de-Souza, Emanuel Schwarz, Hassan Rahmoune, Murtada Alsaif, Jakub Tomasik, Christoph W Turck, Sabine Bahn
Journal: Genome Medicine (2013)

Schizophrenia is a heterogeneous psychiatric disorder characterized by an array of clinical manifestations. Although the best known manifestations include serious effects on mood and behavior, patients can also display co-morbidities, including immune system or metabolic abnormalities. Thorough characterization of these conditions using proteomic profiling methods has increased our knowledge of these molecular differences and has helped to unravel the complexity and heterogeneity of this debilitating condition. This could lead to patient stratification through characterization of biochemically different subtypes of the disease. In addition, proteomic methods have recently been used for molecular characterization of the mechanism of action of antipsychotic medications in both preclinical models and patients. This has resulted in identification of molecular panels that show some promise for prediction of response or for monitoring treatment outcome. This review describes how proteomic profiling methods can impact the future of schizophrenia diagnosis and therapeutics, and facilitate personalized medicine approaches for more effective treatment management of schizophrenia patients.
The role of genetic polymorphisms in environmental health.
Authors: Samir N Kelada, David L Eaton, Sophia S Wang, Nathaniel R Rothman, Muin J Khoury
Journal: Environmental Health Perspectives (2003)

Interest is increasing in the role of variations in the human genome (polymorphisms) in modifying the effect of exposures to environmental health hazards (often referred to as gene-environment interaction), which render some individuals or groups in the population more or less likely to develop disease after exposure. This review is intended for an audience of environmental health practitioners and students and is designed to raise awareness about this rapidly growing field of research by presenting established and novel examples of gene-environment interaction that illustrate the major theme of effect modification. Current data gaps are identified and discussed to illustrate limitations of past research and the need for the application of more robust methods in future research projects. Two primary benefits of incorporating genetics into the existing environmental health research framework are illustrated: a) the ability to detect different levels of risk within the population, and b) greater understanding of etiologic mechanisms. Both offer opportunities for developing new methods of disease prevention. Finally, we describe a basic framework for researchers interested in pursuing health effects research that incorporates genetic polymorphisms.
The treatment of port-wine stains with the pulsed-dye laser at 2-week and 6-week intervals: a comparative study
Authors: N. Tomson, S.P.R. Lim, A. Abdullah, S.W. Lanigan
Journal: British Journal of Dermatology (2006)

Journal Article The treatment of port‐wine stains with the pulsed‐dye laser at 2‐week and 6‐week intervals: a comparative study Get access N. Tomson, N. Tomson Department of Dermatology, Birmingham Skin Centre and LaserCare, City Hospital, Birmingham B18 7QH, U.K Search for other works by this author on: Oxford Academic Google Scholar S.P.R. Lim, S.P.R. Lim Department of Dermatology, Birmingham Skin Centre and LaserCare, City Hospital, Birmingham B18 7QH, U.K Su Ping Regina Lim. E‐mail: reglim@doctors.org.uk Search for other works by this author on: Oxford Academic Google Scholar A. Abdullah, A. Abdullah Department of Dermatology, Birmingham Skin Centre and LaserCare, City Hospital, Birmingham B18 7QH, U.K Search for other works by this author on: Oxford Academic Google Scholar S.W. Lanigan S.W. Lanigan Department of Dermatology, Birmingham Skin Centre and LaserCare, City Hospital, Birmingham B18 7QH, U.K Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 154, Issue 4, 1 April 2006, Pages 676–679, https://doi.org/10.1111/j.1365-2133.2005.07113.x Published: 01 April 2006 Article history Accepted: 28 September 2005 Published: 01 April 2006
Relationship of Body Fat Distribution Pattern to Atherogenic Risk Factors in NIDDM: Preliminary Results
Authors: Luc Van Gaal, Eddy Rillaerts, Wouter Creten, Ivo De Leeuw
Journal: Diabetes Care (1988)

Because recent knowledge indicates that the distribution of fat deposits in men may be a better predictor of cardiovascular disease than the degree of obesity alone, some risk factors for atherosclerosis were evaluated in 51 middle-aged men with non-insulin-dependent diabetes mellitus. Abdominal adiposity (waist/hip ratio, WHR) was related to parameters of metabolic control, lipid parameters, and known vascular complications in three different groups. In groups with abdominal obesity, mean annual hemoglobin A1 was significantly (P less than .01) higher than in patients without an abdominal fat distribution. Atherogenic index was significantly increased in the group with the highest WHR and high-density lipoprotein cholesterol (HDL-chol) levels were significantly decreased in both groups with upper-body fat distribution. The frequency of peripheral vascular disease, coronary ischemic heart disease, and hypertension was most prominent in diabetic subjects with an abdominal fat mass distribution. A highly significant (P less than .001) correlation was present between WHR and HDL-chol and WHR and the total-cholesterol/HDL-chol ratio; this significant correlation remains after correction for body mass index. A similar correlation could be found between WHR and systolic and diastolic blood pressures. These results demonstrate an association of excess abdominal fat, even without manifest obesity, with worse diabetes metabolic control, cardiovascular complications, and blood lipid levels actually considered to play an important role in atherogenesis.