tbuckley/cabot-search huggingface dataset:
Role of nociceptin orphanin FQ peptide - receptor system in mast cell migration
Authors: Shailendra Singh, Nikol Sullo, Peter Bradding, Bruno D'Agostino, Christopher Brightling, David Lambert
Journal: European Respiratory Journal (2013)

Asthma is characterised by airway inflammation, airflow obstruction and bronchial hyper-responsiveness. Mast cells play a key role in the pathophysiology of asthma. The heptadecapeptide nociceptin/orphanin FQ (N/OFQ) is the endogenous ligand for N/OFQ peptide (NOP) receptor. Animal studies suggest that N/OFQ-NOP system modulates airway inflammation. Whether mast cells express functional N/OFQ-NOP is unknown.  We investigated NOP and ppN/OFQ mRNA expression in human lung mast cells (HLMC) relative to GAPDH mRNA (housekeeping gene) by qRT-PCR and the findings were expressed as Δ CT. NOP protein expression was determined by [125I]N/OFQ saturation binding assays and expressed as receptor density (Bmax) in terms of fmol bound/mg protein. The role of N/OFQ on HLMC migration and proliferation was investigated.  No ppN/OFQ mRNA expression was detected. HLMC and HMC-1 cells expressed NOP mRNA with mean Δ CT values of 6.99 ± 0.64 (n=5) and 6.58 ± 1.14 (n=5) respectively. Saturation binding assays detected low NOP protein expression in HMC-1 cells (mean Bmax = 16.99 ± 5.92, n=3) relative to a cell line expressing recombinant human NOP (mean Bmax = 1321.38 ± 60.26, n=4). Transwell migration assays demonstrated that stem cell factor (SCF) induced a 2.29 ± 0.4 (n=5) fold increase in HLMC migration over the control and this was significantly inhibited by N/OFQ (0.98 ± 0.08, n=5, p  Our findings demonstrated that HLMC express NOP. N/OFQ significantly inhibited SCF-induced HLMC migration suggesting a potential role for N/OFQ in regulating airway inflammation.
Asthma medication use in the general population of West Sweden
Authors: Linda Ekerljung, Anders Bjerg, Göran Wennergren, Bo Lundbäck
Journal: European Respiratory Journal (2013)

Background: Many studies focus on the use of asthma medication in clinical cohorts of asthmatics, but very few study the use of asthma medication in the general population. The aim of the study was to identify patterns of asthma medication use in a general adult population.  Methods: From a randomly selected population, aged 16-75 years, who participated in a postal survey on respiratory symptoms, a random sample of 2000 was selected for clinical examinations and interview, including questions on asthma medication use. In total 1172 participated.  Results: Physician-diagnosed asthma (asthma) was reported by 11% and physician-diagnosed COPD (COPD) by 2%. Recurrent wheeze was reported by 9%, attacks of shortness of breath by 8% and chronic productive cough by 7%. Asthma medication was used by 11% of the population. The most common asthma medication was short-acting beta-2 agonists (SABA) which was used by 8%. A majority of SABA users, 63%, also used inhaled corticosteroids (ICS). Most users of SABA used their medication occasionally, but 17% of subjects only using SABA used it most days. ICS was used by 4% and an additional 4% used a combination treatment with ICS and LABA. Of users of combination treatment, 71% used their medication on most days, compared with 58% among users of ICS. Asthma medication was used by 71% of subjects with asthma, 92% of subjects who had visited an asthma specialist and 68% of subjects reporting COPD. ICS was used by 55% of subjects with asthma and 53% of subjects reporting COPD.  Conclusion: Use of asthma medication is common. Half of the asthmatic subjects in a general population used ICS. One fifth of subjects who only use SABA used them most days and could probably benefit from use of ICS.
Decametoxine influence on bronchial hyperreactivity in patients with bronchial asthma
Authors: Mykola Gumeniuk, Svitlana Panchuk
Journal: European Respiratory Journal (2014)

Background. In vitro 0,02% sol. decametoxine has bactericidal and virucidal action against the main viral and bacterial pathogens of infectious exacerbation of asthma, suggesting its clinical effectiveness in patients with infectious exacerbation of asthma.  At the same time, in vivo decametoxine inhalation may enhance bronchial hyperresponsiveness and bronchial obstruction. Therefore, the aim of this study was to evaluate the influence of 0.02% sol. decametoxine on bronchial hyperreactivity in patients with asthma.  Methods. Spirography was conducted in 26 patients with bronchial asthma (11 men and 15 women aged from 29 to 63 years) in whom confirmation of obstruction reversibility was performed with an inhaled bronchodilator (salbutamol 400 mcg). Next day respiratory function was evaluated before 0.02% sol. decametoxine inhalation (4 ml via nebulizer) and on 15 min and 45 min after inhalation. To determine the reliability of changes in respiratory function ( FVC, FEV1, FEV1/ FVC ) the difference between their absolute values, the initial data and expressed as a percentage of the proper size was calculated. The results are compared with a range of reproducibility of these indicators. Dynamics studies considered significant (positive or negative), if the shift parameters FVC , FEV1 , FEV1/FVC exceeded their reproducibility.  All measurements were performed in the first half of the day on the same apparatus by the same investigator.  Results: Respiratory function parameters showed that decametoxine inhalation did not significantly altere respiratory function.  Conclusions: Decametoxine inhalation do not affect bronchial hyperreactivity, which allows for the possibility of its use in patients with asthma.
Humoral Immune Responses to Tumor-Specific Antigens in Strain-2 Guinea Pigs
Authors: Howard G. Smith, Edward J. Leonard
Journal: JNCI Journal of the National Cancer Institute (1974)

Serum antibody against tumor-specific antigens on the surface of line-10 guinea-pig hepatoma cells was demonstrated by equilibrating serum with viable tumor cells and detecting bound antibody by indirect immunofluorescence. Summation of fluorescence intensity grades of individual cells led to a semiquantitative assay of antibody activity sufficiently precise for studies on the magnitude and time course of antibody response. Antibody was found not only in guinea pigs immunized against line-10 tumor with a line-10 BCG vaccine, but also in animals with progressively growing and metastasizing tumors. All animals studied had a primary humoral response to line-10 BCG vaccine and generally developed higher antibody levels after multiple inoculations of tumor cells. Immunization with line-10 antigen extracts in complete Freund adjuvant produced delayed cutaneous hypersensitivity responses, but no humoral antibody was detected until after challenge with viable tumor cells, when an apparent anamnestic antibody response was seen. Guinea pigs with progressively growing line-10 tumors developed antibody after 4-5 weeks of tumor growth. Before antibody was detected, their serum inhibited the binding of antitumor antibody to line-10 cells.
Effect of a Low-Fat Diet on Hormone Levels in Women With Cystic Breast Disease. II. Serum Radioimmunoassayable Prolactin and Growth Hormone and Bioactive Lactogenic Hormones&lt;xref ref-type="fn" rid="FN2"&gt;2&lt;/xref&gt;
Authors: D P Rose, L A Cohen, B Berke, A P Boyar
Journal: JNCI Journal of the National Cancer Institute (1987)

For investigation of the bioactivity of circulating prolactin and growth hormone (lactogenic hormones) in symptomatic benign breast disease, serum was assayed by the Nb2 lymphoma cell method in premenopausal patients with cystic breast disease and cyclic mastalgia and in normal premenopausal women. The results were compared with serum prolactin and growth hormone concentrations determined by radioimmunoassay. The serum bioassayable hormone levels in the benign breast disease patients (74.0±77.6 ng/ml) were significantly higher (P<.001) than in normal women (23.8±10.7 ng/ml). There were no significant differences in the radioimmunoassayable prolactin or growth hormone levels between the 2 groups. When 16 cystic breast disease patients were placed on a low-fat (20% of total kilocalories) diet for 3 months, there were significant reductions in the serum bioassayable hormone levels (P<.02). It is concluded that the bioactivity of prolactin may be elevated in the serum of patients with cystic breast disease and cyclic mastalgia, without corresponding increases in levels determined by radioimmunoassay; that this abnormality is reversible by a reduction in dietary fat consumption to 20% of the total kilocalories; and that serum prolactin may provide a valuable biomarker in clinical trials of a low-fat diet in women at high breast cancer risk.
Abstract 15763: FoxO1 Drives VCAM1 Expression to Govern Chorioallantoic Fusion and Early Cardiac and Vascular Development
Authors: Anwarul Ferdous, Jesse Morris, Md. Joynal Abedin, Shandon C Collins, James A Richardson, Joseph A Hill
Journal: Circulation (2010)

Introduction: Targeted disruption of FoxO1, a member of the Forkhead box-containing O family of transcription factors, leads to embryonic lethality on embryonic day 10.5 (E10.5) due to abnormal car...
Serum Action on Radiation Response in Tissue Culture
Authors: Hector Montes Oca, Edward L. Schilling, Jay C. Bryant, Virginia J. Evans, Wilton R. Earle
Journal: JNCI Journal of the National Cancer Institute (1963)

This study explores the action of serum in the radiation response of strain NCTC 2071 cells cultured in a medium free of serum or protein through more than 294 subculture generations for over 6 years. Since it is difficult, if not impossible, to wash serum completely from cells, even with multiple saline rinses, cells of this strain were ideal for this study. Cells in agitated fluid-suspension cultures on the Brunswick shaker for 28 days were used. It was shown that serum modified the radiation response by speeding recovery of the irradiated cultures when serum was added either before or immediately after irradiation; such cells began to recover at 10 days after irradiation. Cells irradiated in medium NCTC 109 without serum, however, usually did not resume growth, or if they did, it was only after 28 days. The mechanism of this action of the serum is discussed.
Spontaneous Tumors and Common Diseases in Two Colonies of Syrian Hamsters. IV. Vascular and Lymphatic Systems and Lesions of Other Sites2
Authors: Parviz Pour, Ulrich Mohr, Jürgen Althoff, Antonio Cardesa, Norbert Kmoch
Journal: JNCI Journal of the National Cancer Institute (1976)

Journal Article Spontaneous Tumors and Common Diseases in Two Colonies of Syrian Hamsters. IV. Vascular and Lymphatic Systems and Lesions of Other Sites Get access Parviz Pour, Parviz Pour Search for other works by this author on: Oxford Academic PubMed Google Scholar Ulrich Mohr, Ulrich Mohr Search for other works by this author on: Oxford Academic PubMed Google Scholar Jürgen Althoff, Jürgen Althoff Search for other works by this author on: Oxford Academic PubMed Google Scholar Antonio Cardesa, Antonio Cardesa Search for other works by this author on: Oxford Academic PubMed Google Scholar Norbert Kmoch Norbert Kmoch Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 56, Issue 5, May 1976, Pages 963–974, https://doi.org/10.1093/jnci/56.5.963 Published: 01 May 1976 Article history Received: 12 May 1975 Revision received: 09 October 1975 Accepted: 21 November 1975 Published: 01 May 1976
Abstract 2600: Serum Proteomics Identifies Vitamin D-Binding Protein And Prothrombin As Novel Fingerprints of Coronary Thrombosis in Patients with STEMI
Authors: Cosimo Gasparri, Antonio Curcio, Marco Gaspari, Paola Mercurio, Carla Vicinanza, Valentina Galuppo, Angelo Leone, Anna Lucia Cavaliere, Aglaia Niola, Duino Boncompagni, Daniele Torella, Giovanni Cuda, Ciro Indolfi
Journal: Circulation (2007)

One of the intriguing possibilities offered by proteomics analysis is to define new diagnostic and prognostic biomarkers of coronary artery disease. Accordingly, the aim of the present study was to analyze modifications in the serum proteome after acute myocardial infarction. Serum samples were collected from patients (age 63.8±12.6) with ST-Elevation Myocardial Infarction (STEMI) at 2–11 hours after the onset of typical chest pain and before standard therapy was initiated. The control group included 10 age- and sex-matched normal donors. The serum samples were processed to obtain albumin- and IgG-depleted serum. Then, isotope-coded affinity tag (ICAT) method was employed to label cysteine residues and liquid chromatography-Tandem Mass Spectrometry (LC-MS/MS) analysis was performed to measure the labelled proteins. Ten out of 300 defined and analyzed proteins were identified in 100% of the samples. Interestingly, haptoglobin, alpha-1-antitrypsin and ceruloplasmin were significantly increased in the samples from patients with acute STEMI (N=10) compared to controls (N=10). These data well reproduce similar findings reported in recent studies. However, two novel proteins were identified in patients with AMI. Remarkably, we observed a significant increase of vitamin D-binding protein precursor (VDB) and heavy chain prothrombin precursor in the serum from acute STEMI patients compared to control donors. Western blot analysis confirmed the higher amount of the latter two proteins after STEMI. Interestingly, fresh thrombotic plaques, obtained during primary angioplasty, showed high expression of VDB and prothrombin by RT-PCR and immunohistochemistry, postulating a potential role of VDB and prothrombin in coronary plaque instability and thrombosis. In additional studies in ex vivo human platelets VDB, as well as prothrombin, significantly increased ADP-induced platelet aggregation. In conclusion, the serum proteomics analysis employed in this study was able to identify VDB and prothrombin protein variation as novel indicators of acute STEMI. These data could be relevant to establish specific proteomics-based serum fingerprints for diagnosis and prognosis of acute coronary syndromes.
A multimodality treatment approach to post transplant airway stenosis
Authors: Lonny Yarmus, John McDyer, Ashish Shah, David Feller-Kopman
Journal: European Respiratory Journal (2011)

Purpose:  Anastomotic airway complications are a significant cause for morbidity and mortality after lung transplantation (LT). The aim of this study was to investigate post LT anastomotic airway complications to evaluate both the need for and modalities used for treatment.   Methods and materials:  All patients undergoing LT performed between August 2005 and May 2010 by a single surgeon at our institution were reviewed retrospectively. All airway complications were managed by the Interventional Pulmonary team. Airway stenoses were managed with rigid bronchoscopy, balloon dilation, observation and or stent placement.   Results:  A total of 98 patients underwent LT (86 bilateral, 7 left, 5 right). 46 female and 53 male, with a mean age 49.7. 28 patients (28.6%) developed pneumonia within three months of transplantation, 8 of which were identified as a result of pseudomonas (28.6%). 28 (28.6%) patients also reported having evidence of acute cellular rejection within three months after transplantation. The median length of mechanical ventilation after transplant was 3 days (range 1 to 183). Of the 98 patients, 35 (35.7%) developed airway stenosis at a median of 16 weeks (range 1 to 164 weeks following surgery). Of these patients, 23 (65.7%) patients received at least one airway intervention.   Conclusions:  Airway stenosis after LT can be successfully managed with a variety of airway interventions. Further studies are needed to determine the best modalities.
Abstract 3658: The Prevalence of Depression and Anxiety among Persons Aged 45 Years and Older by Cardiovascular disease Status, 2006
Authors: Amy Z Fan, Tara W Strine, Ruth Jiles, Ali H Mokdad
Journal: Circulation (2007)

Depression and anxiety commonly occur after nonfatal cardiovascular events. It is imperative to learn the extent of depression and anxiety among middle-aged and elder adults with cardiovascular disease at the population level. The Behavioral Risk Factor Surveillance System (BRFSS) is an ongoing, state-based, telephone survey of non-institutionalized adults. In 2006, an Anxiety and Depression Module (ADM) was administered in 38 States, Puerto Rico, the US Virgin Islands, and the District of Columbia. Eight ADM questions were adapted from the validated Patient Health Questionnaire 8. A standardized summary depression severity score from the eight questions was dichotomized as ≥10 or &lt;10. A score ≥10 has been shown to have an 88% sensitivity and specificity for major depression. Two questions assessed lifetime diagnosis of depressive and anxiety disorders. Cardiovascular disease (CVD) was assessed using three questions on coronary heart disease (including angina and myocardial infarction) and stroke. The analysis was performed using SUDAAN 9.0 to account for the complex sampling design of BRFSS. Adjusted prevalence ratio (APR) was obtained by the LOGLINK procedure after adjustment for age, sex, race/ethnicity, education level, marital status and employment status. Results showed among persons aged 45 years and older (n=129,499), the prevalence of a CVD history was 15.3% (95% CI=14.9%-15.7%). The prevalence of CHD and stroke was 12.4% (95% CI=12.0%-12.7%) and 5.0% (95% CI=4.8%-5.2%), respectively. The prevalence of a current depression severity score ≥10 was 7.7% (95% CI=7.3%-8.0%). The prevalence of a lifetime diagnosis of depressive and anxiety disorders was 16.2% (95% CI=15.8%-16.6%) and 11.0% (95% CI=10.6%-11.3%) respectively. Persons with a CVD history were more likely to have a depression severity score ≥10 (15.8% vs. 7.1%, APR [95% CI] = 1.69 [1.54 –1.85]) or have a lifetime diagnosis of depressive (22.3% versus 15.1%, APR [95% CI] = 1.56 [1.45–1.67]) or anxiety disorders (16.6% versus 10.0%, APR [95% CI] = 1.46 [1.37–1.54]). The findings underscore the importance of assessing mental health status in chronic care and management of cardiovascular disease.
Complex Intrathoracic Tracheal Injury
Authors: Michael W. Wandling, Andrew W. Hoel, Shari L. Meyerson, Heron E. Rodriguez, Michael B. Shapiro, Mamta Swaroop, Ankit Bharat
Journal: American Journal of Respiratory and Critical Care Medicine (2015)

tracheal repair, the endotracheal tube was replaced and positioned just proximal to the anastomosis.Subsequently, innominate artery resection and creation of ascending aorta to distal innominate bypass was performed using a rifampin-soaked polyester graft.A vascularized pedicled flap of pericardium with accompanying fat pad was interposed.Tracheobronchial injuries are rare but life-threatening injuries in blunt trauma (1).Approximately 90% are intrathoracic and occur in the distal trachea or mainstem bronchi within 2.5 cm of the carina (2).Signs and symptoms of tracheobronchial injury include subcutaneous emphysema, dyspnea, hemoptysis, pneumomediastinum, and pneumothorax (2).Presentation ranges from asymptomatic, as observed in this case, to severe respiratory distress or death.Securing an airway in the setting of tracheobronchial injury is challenging but of utmost importance.Intubation should be performed under bronchoscopic guidance to minimize additional airway damage.If possible, the patient should be awake, and neuromuscular blockade should be avoided.Cardiopulmonary bypass can be life saving if difficulty is encountered in securing the airway (3) and extremely valuable in the surgical repair of complex tracheobronchial injuries (4).Treatment options include surveillance, bronchoscopic stenting, t-tube placement, primary repair, and tracheal resection (5, 6).In cases of complete disruption, as illustrated here, debridement of devascularized edges with end-to-end anastomosis is preferred (5).Nonoperative management is reserved for asymptomatic patients with injuries limited to less than one-third of the trachea (7).
Deaths From Brain Tumors in Eastern Kentucky, 1950–69
Authors: Edward T. Creagan, Joseph F. Fraumeni
Journal: JNCI Journal of the National Cancer Institute (1973)

Journal Article Deaths From Brain Tumors in Eastern Kentucky, 1950–69 Get access Edward T. Creagan, M.D., Edward T. Creagan, M.D. Epidemiology Branch, National Cancer Institute, National Institutes of Health, Public Health Service, U.S. Department of Health, Education, and Welfare, Bethesda, Md. 20014 Search for other works by this author on: Oxford Academic PubMed Google Scholar Joseph F. Fraumeni, Jr., M.D. Joseph F. Fraumeni, Jr., M.D. 4 Epidemiology Branch, National Cancer Institute, National Institutes of Health, Public Health Service, U.S. Department of Health, Education, and Welfare, Bethesda, Md. 20014 4Address reprint requests to Dr. Fraumeni, Landow Bldg., Room A521, National Cancer Institute. Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 51, Issue 5, November 1973, Pages 1717–1718, https://doi.org/10.1093/jnci/51.5.1717 Published: 01 November 1973 Article history Received: 31 July 1973 Accepted: 31 July 1973 Published: 01 November 1973
Primary Nonadherence in Acne Treatment: The Importance of Cost Consciousness
Authors: Courtney B. Rubin, Jules B. Lipoff
Journal: JAMA Dermatology (2015)

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