tbuckley/cabot-search huggingface dataset:
657. A Nucleic Acid-Based Transgene Reporter for In Vivo MRI
Authors: Eric T. Ahrens, Clinton S. Robison, William F. Goins
Journal: Molecular Therapy (2005)

Top of pageAbstract
The ability to non-invasively image the duration and location of gene expression in vivo is important for the future of biomedical research and molecular medicine. We describe a new platform technology for visualizing transgene expression in living subjects using MRI. We introduced an MRI reporter, the human ferritin (FT) metalloprotein, into host neural tissues using a replication-defective adenovirus (AdV) vector. The reporter was made potent as the transduced cells sequestered endogenous iron from the organism, resulting in detectable MRI contrast. In this novel approach, the cells constructed the MRI contrast agent in situ via genetic instructions introduced by the vector. No exogenous metal-complexed contrast agent was required, thereby simplifying intracellular delivery. We initially characterized the biological effects of the vector-mediated MRI reporter expression in a detailed series of assays in A549 cells in culture. ELISA demonstrated high-level FT expression from the AdV-FT transduced cells. The AdV-FT transduced cells consistently showed increased 59Fe uptake compared to AdV-lacZ controls following the addition of 59Fe-enriched transferrin to the medium, indicative of greater iron uptake and storage capacity. To detect relative Fe loading in transduced cells in vitro, we measured 1/T2 in pelleted A549 cells and found significantly enhanced 1/T2 by approximately 2.5-fold in the MRI reporter-transduced cells compared to the controls. T2-weighted MRI of A549 cell pellets displayed easily discernable contrast between AdV-FT and AdV-lacZ transduced cells. MTT cellular proliferation assays and G6PD cytotoxicity assays demonstrated no statistically significant difference in cell viability or toxicity compared to controls. Following focal inoculation of virus into the striatum of the C57Bl/6J mouse brain, we monitored the time-lapse reporter activity for several weeks in vivo using T2- and T2|[ast]|-weighted anatomical MRI. We observed robust contrast in virus-transduced neurons and glia. Histology and immunohistochemistry was used to confirm the results in the same animals after MRI. This technology is adaptable to monitor transgene expression in vivo in many tissue types and has numerous applications, such as visualizing preclinical therapeutic gene delivery to assess dosage and delivery protocols.
The benefit of an early invasive strategy in women versus men with non-ST-elevation acute coronary syndromes: A collaborative meta-analysis of randomized trials
Authors: M O'Donoghue, Sabatine, WE Boden, E Braunwald, CP Cannon, TC Clayton, RJ de Winter, KA Fox, PA McCullough, SA Murphy, R Spacek, E Swahn, L Wallentin, F Winchausen
Journal: Circulation (2007)
Abstract 12205: Saving Lives in Schools — School-based CPR-AED Programs: Awareness, Education, Planning and Partnerships
Authors: Stuart Berger, Robert Campbell, Jon Drezner, John Wilson, Allison Ellison, Maryanne Kessel, Debra Klich, Rebecca Neumann-Schwabe
Journal: Circulation (2010)

Background: Sudden cardiac death (SCD) in the young is devastating. 20% of the U.S. adult and pediatric population spends time in schools each week, making schools a strategic location for secondary prevention through CPR-AED programs. Purpose: We sought to describe the impact of school based CPR-AED programs. Methods: Project ADAM (PA) (Milwaukee, WI) and Project SAVE (Atlanta, GA) are community programs designed to prevent SCD in schools. The goals are directed toward schools, including (1) education/awareness of signs, symptoms, treatment of SCA, (2) implementation of CPR-AED programs in schools (3) advocacy for students to learn CPR/AED use prior to graduation. SCA events are monitored at existing participating schools. A comprehensive program includes a coordinator, EMS, first responder team development/training, purchase/maintenance of AED(s) and an emergency response plan. Results: WI: 850 schools have CPR-AED programs (35% of all WI schools). All schools in the Milwaukee Public School system have ...
A case of false-negative monochromator phototesting in a patient with chronic actinic dermatitis taking prednisolone
Authors: J. Ferguson, S.H. Ibbotson
Journal: British Journal of Dermatology (2012)

Journal Article A case of false‐negative monochromator phototesting in a patient with chronic actinic dermatitis taking prednisolone Get access J. Ferguson, J. Ferguson Department of Dermatology, Photobiology Unit, Ninewells Hospital and Medical School, Dundee DD1 9SY, U.K. E‐mail: j.ferguson@dundee.ac.uk Search for other works by this author on: Oxford Academic Google Scholar S.H. Ibbotson S.H. Ibbotson Department of Dermatology, Photobiology Unit, Ninewells Hospital and Medical School, Dundee DD1 9SY, U.K. E‐mail: j.ferguson@dundee.ac.uk Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 167, Issue 1, 1 July 2012, Pages 214–215, https://doi.org/10.1111/j.1365-2133.2012.10834.x Published: 01 July 2012
Electrocardiographic Findings in Non–ST-Segment Elevation Myocardial Infarction
Authors: Amnon Schlegel
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract P2-13-10: Assessment of diagnostic and therapeutic value of ductoscopy biopsy in single intraductal papillary lesion compared with open surgery
Authors: Zheli Xu, Wanying Xing, Qiang Li, Deli Xing, Yiqi Gu
Journal: Cancer Research (2015)

Abstract Purpose Papillary lesions (PL) account for 1–2 % of all breast neoplasms and fiberoptic ductoscopy is a practical and direct method compared with open biopsy in diagnosis and treatment of intraductal papillary lesions. This study aimed to assess efficacy of ductoscopy in the diagnosis and management of single intraductal papillary lesion. Methods A total of 232 patients at China-Japan Union Hospital of Jilin University who were diagnosed with single intraductal papillary lesion by fiberoptic ductoscopy were enrolled from March 2011 to November 2013. All patients underwent ductoscopic papillomectomy before open surgery. The final pathologic diagnoses were made by using specimen from both ductoscopic papillomectomy and surgeries. Any intraductal papillomatous lesion or surface abnormalities were considered as positive findings during ductoscopy. Results Histopathologic investigations of surgically excised or ductoscopically removed lesions revealed that 217 out of 232 cases were positive. In 187 cases pathological changes were only found in ductoscopic papillomectomy specimen, while the number of cases which was only found in open biopsy was 24. In 6 cases ductoscopic papillomectomy and surgeries specimen both showed positive. Conclusions The accuracy of ductoscopy biopsy in diagnosis was 88.9% (193/217) and specificity was 100%. In 187 out of 217 cases (86.2%) ductoscopic papillomectomy alone could completely remove the lesion. Ductoscopy is an effective tool for diagnosis and treatment of single intraductal papillary lesion. Citation Format: Zheli Xu, Wanying Xing, Qiang Li, Deli Xing, Yiqi Gu. Assessment of diagnostic and therapeutic value of ductoscopy biopsy in single intraductal papillary lesion compared with open surgery [abstract]. In: Proceedings of the Thirty-Seventh Annual CTRC-AACR San Antonio Breast Cancer Symposium: 2014 Dec 9-13; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2015;75(9 Suppl):Abstract nr P2-13-10.
Abstract 352: Juvenile Doxorubicin Treatment Impairs Recovery and Promotes Apoptosis in Adult Hearts via failure of up-regulation of Mcl-1 after Myocardial Infarction
Authors: Chengqun Huang, Melissa N Quinsay, Lucy Kim, Dieter A Kubli, Åsa B Gustafsson
Journal: Circulation (2008)

The anthracycline doxorubicin (DOX) is an effective chemotherapeutic agent used to treat pediatric cancers. However, it is associated with cardiotoxicity which can manifest many years after the initial exposure. Very little is known about the mechanisms of this late-onset cardiotoxicity. To understand this problem, we have developed a model of pediatric DOX cardiotoxicity where mouse pups were injected with 4 low doses of DOX or saline at 5, 10, 15, and 20 days of age. After completion of treatment, all mice were healthy and had normal cardiac function as adults. However, when mice were subjected to myocardial infarction (MI), DOX treated mice developed severe cardiac fibrosis and had increased mortality compared to saline treated mice (67% vs. 20%), suggesting that these hearts do not tolerate stress well. Assessment of infarct size revealed that infarct size was greater in DOX-treated hearts compared to saline. Anthracyclines are known to affect the Bcl-2 family proteins which are also important regulators of cell death in the heart. We found that Mcl-1, an anti-apoptotic Bcl-2 protein, is expressed in the heart and localizes to the mitochondria. Overexpression of Mcl-1 in HL-1 myocytes reduced simulated I/R-mediated cell death (58±8% vs. 26±7%, n=3, p&lt;0.05), suggesting that Mcl-1 promotes survival of cardiac cells in response to stress. Immunostaining analysis of Mcl-1 expression in mouse hearts revealed that Mcl-1 was significantly increased in myocytes in the border zone after acute MI. Myocytes with enhanced Mcl-1 expression were negative for apoptosis as assessed by TUNEL staining, whereas cells with reduced Mcl-1 expression were TUNEL positive. Since anthracyclines have been shown to reduce Mcl-1 expression in cancer cells, we investigated whether juvenile DOX treatment would suppress Mcl-1 expression in adult heart in response to stress. We found that cardiac myocytes in the border zone of DOX treated hearts failed to upregulate Mcl-1 in response to MI which likely impaired recovery and contributed to increased injury and mortality. These studies suggest upregulation of Mcl-1 is an important salvage response after MI and that juvenile DOX treatment affects Mcl-1 expression in adulthood resulting in a heart that is more susceptible to stress. This research has received full or partial funding support from the American Heart Association, AHA Western States Affiliate (California, Nevada &amp; Utah).
Abstract P306: Increase of the Inflammatory Burden at a Young Age in Patients with Coronary Artery Disease
Authors: Enkhmaa Byambaa, Anuurad Erdembileg, Wei Zhang, Kyoungmi Kim, Lars Berglund
Journal: Circulation (2015)

Introduction: Age and inflammation both are considered risk factors for coronary artery disease (CAD) and the levels of inflammatory markers increase with aging. However, the role of age-induced inflammatory burden in CAD development is not fully understood. We investigated the trajectories of inflammatory markers with increasing age in subjects with or without CAD. Hypothesis: We hypothesized that the rate of increase in inflammatory markers as a function of age would depend on the presence of CAD and differ between subjects with and without CAD. Methods: Systemic inflammatory burden was assessed by levels of C-reactive protein (CRP), serum amyloid A (SAA) and fibrinogen in Caucasians (n=184) and African-Americans (n=154) (age range: 20-60 years). The presence of CAD was defined as the presence of ≥50% stenosis in any one of 15 coronary artery segments. Results: The prevalence of CAD was 46% (85 of 184) and 38% (58 of 154) in Caucasians and African-Americans, respectively. In both ethnic groups, subjects with CAD were older and had an atherogenic lipid profile compared to subjects without CAD. For both subjects with and without CAD, HDL cholesterol level was significantly higher and triglyceride level was significantly lower in African-Americans compared to Caucasians. The pattern of inflammatory burden over age differed across CAD status and ethnicity. Among Caucasians, the trend patterns of CRP (p&lt;0.05), SAA (p&lt;0.01) and fibrinogen (p&lt;0.001) over age differed significantly between subjects with and without CAD. Levels of all three markers were elevated already at young ages and remained relatively constant over age in Caucasians with CAD. In contrast, in Caucasians without CAD, the levels of these markers were lower at young ages with a gradual increase over age. The findings for African-Americans differed from that of Caucasians. The levels of inflammatory markers increased with age with no significant differences in trends between subjects with and without CAD. In multivariate analyses adjusting for covariates, the trend pattern of fibrinogen over age differed significantly between Caucasians with and without CAD. Conclusions: There was a diverging pattern in age-induced inflammatory burden associated with CAD, with an early start and faster rate of increase in Caucasian patients with CAD. The findings suggest an accelerated inflammation over age in individuals with CAD with a possible modulatory role of ethnicity/race.
Vismodegib in patients with advanced basal cell carcinoma (STEVIE): a pre-planned interim analysis of an international, open-label trial
Authors: Nicole Basset-Seguin, Axel Hauschild, Jean-Jacques Grob, Rainer Kunstfeld, Brigitte Dréno, Laurent Mortier, Paolo A Ascierto, Lisa Licitra, Caroline Dutriaux, Luc Thomas, Thomas Jouary, Nicolas Meyer, Bernard Guillot, Reinhard Dummer, Kate Fife, D Scott Ernst, Sarah Williams, Alberto Fittipaldo, Ioannis Xynos, Johan Hansson
Journal: The Lancet Oncology (2015)
Treatment of verrucous carcinoma of vulva with acitretin
Authors: R.K. Mehta, E. Rytina, J.C. Sterling
Journal: British Journal of Dermatology (2000)

Abstract We describe a 60-year-old patient with verrucous carcinoma of the vulva, which recurred 6 years after simple vulvectomy and radiotherapy. Treatment with acitretin led to significant improvement and ongoing disease control has been achieved with low-dose maintenance therapy of 10 mg acitretin daily.
IOM Report on Cognitive Aging
Authors: Julie Jacob
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Journals JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2024 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Estimation of Life-Years Saved by Solid-Organ Transplant
Authors: Meredith C. Foster, Narittaya Varothai, Daniel E. Weiner
Journal: JAMA Surgery (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal
Abstract 1207: In Vivo Molecular Imaging Links Macrophage Burden and Microcalcifications in Early Atherosclerosis
Authors: Elena Aikawa, Matthias Nahrendorf, Jose-Luiz Figueiredo, Filip K Swirski, Timur Shtatland, Rainer Kohler, Farouc A Jaffer, Masanori Aikawa, Ralph Weissleder
Journal: Circulation (2007)

Backround Microcalcifications may provoke plaque rupture leading to acute cardiovascular events. However, the mechanisms that initiate arterial calcification remain obscure. Methods and Results We tested the hypothesis that inflammation promotes osteogenesis in atherosclerotic plaques using in vivo molecular imaging in apoE −/− mice (n=40). A bisphosphonate-derivatized near-infrared fluorescent (NIRF) imaging agent (750 nm) visualized osteogenic activity that was otherwise undetectable by X-ray computed tomography. Flow cytometry validated target cells as osteoblasts. A spectrally distinct NIRF nanoparticle (680 nm) was coinjected to simultaneously image macrophages. Fluorescence reflectance mapping associated osteogenic activity with inflammation in aortas of apoE −/− mice. Intravital dual-channel fluorescent microscopy further monitored osteogenic changes in carotid plaques at 20 and 30 weeks of age and revealed that both macrophage burden and osteogenesis concomitantly increased during plaque progression and decreased after statin treatment (Figure ) . Fluorescence microscopy on cryosections colocalized NIRF osteogenic signals with alkaline phosphatase activity, bone-regulating proteins and hydroxyapatite nanocrystals detected by electron microscopy, while von Kossa staining showed no evidence of calcification. Real-time RT-PCR revealed that macrophage-conditioned media induced alkaline phosphatase mRNA expression in vascular smooth muscle cells. Conclusion This serial in vivo study detects osteogenic activity within macrophage-rich plaques, offering a cellular resolution tool to identify preclinical microcalcifications.
Abstract 15181: Predictors of Mortality and Morbidity in Patients With Chronic Heart Failure - Analysis of Red-HF Trial
Authors: Inder S Anand, Changhong Yu, Marc A Pfeffer, John J McMurray, Akshay S Desai, Scott D Solomon, Dirk J Veldhuisen, Sunfa Cheng, Kurt Olson, Aldo P Maggioni, Karl Swedberg, James B Young
Journal: Circulation (2014)

Background: A number of prognostic models have been developed to risk stratify patients with heart failure (HF) and reduced ejection fraction (EF). However, few have included medications, device therapy and natriuretic peptides. Methods and Results: We analyzed data from 2278 patients in Reduction of Events with Darbepoetin Alfa in Heart Failure (RED-HF) trial that tested the effects raising hemoglobin in high-risk anemic patients with HF (median age 72 years; Hg 11.2 g/dL; EF 31%; and GFR 46 ml/min/1.73m2; annualized mortality 14.1%). Multivariable Cox regression models were developed to predict the primary endpoint of all-cause mortality or hospitalization for HF, and all-cause mortality, starting with 43 baseline demographic, clinical, treatment and biochemical variables including NT-proBNP. Nonlinear relationships with continuous variables were examined using restricted cubic splines. Final predictor variables were selected using backward stepwise selection in 1000 bootstrap samples. For the primary outcome, NT-proBNP, hospitalization for HF in past 6-months, heart rate, diastolic BP and bilirubin were the strongest of the 12 final predictors that provided good discrimination (Harrell’s C=0.74) with excellent calibration (Figure). Surprisingly, age, diabetes and EF were not selected. Forcing them in the model did not change discrimination (Harrell’s C=0.74). For all-cause mortality, NT-proBNP, diastolic BP, beta-blocker use, bilirubin and albumin were among the strongest of the 11 final predictors (Harrell’s C=0.71). Again, diabetes and EF were not in the model and forcing them in the model did not improve the C-statistics. Conclusions: In a contemporary well-treated population of severe HF with reduced EF, a multivariable risk model that included medications, devices and natriuretic peptide provided good discrimination and prediction of the primary outcome and all-cause mortality. NT-proBNP was the strongest predictor of both outcomes.