tbuckley/cabot-search huggingface dataset:
Abstract 15440: Dynamics of Adipocyte and Endothelial Progenitor Cell Pools in Expanding Adipose Tissue
Authors: Candice R Holden, Marcin Wysoczynski, Brian Sansbury, Jason Hellmann, Nagma Zafar, Andrew Gibb, Matthew Spite, Aruni Bhatnagar, Bradford Hill
Journal: Circulation (2014)

Objective: Obesity is a major risk factor for the development of several chronic diseases including type 2 diabetes and cardiovascular disease. Proper fat storage in white adipose tissue (WAT) is required to maintain insulin sensitivity and to preserve (cardio)vascular health. We hypothesize that endothelial and adipocyte progenitor cell populations (EPCs and APCs, respectively) must be appropriately balanced for physiological, as opposed to pathological, remodeling of WAT. Methods and Results: To determine the impact of nutrient excess on stem/progenitor cells in epididymal WAT, male C57BL/6J mice were placed on a high fat diet (HFD; 60% fat) for 12 weeks and changes in WAT stem cell populations were measured in the stromal vascular fraction by flow cytometry. Although the APC (CD24+/CD29+/Sca+/CD14-/CD45-) population, which has the capacity to differentiate into adipocytes both in vitro and in vivo , was not significantly changed with diet, Flk+/Sca+ EPCs were diminished, promoting a 4-fold decrease in the EPC/APC ratio (p &lt;0.05, n = 6/group). To determine whether this deficit may be due to poor stem cell recruitment, mice were irradiated, and the bone marrow was repopulated with GFP+ donor marrow. The transplanted mice were then placed on a low fat diet (LFD; 10% fat) or HFD for 12 weeks, and WAT progenitor cells were again measured. Greater than 95% of the putative APCs in the WAT of HF-fed mice were GFP+ (p&lt;0.0001, n=7-8/group), indicating a bone marrow-derived origin. Unexpectedly, less than 1% of the EPCs were GFP+ (p&lt;0.001, n=7-8/group), which suggests that EPCs present in WAT are not derived from bone marrow in adult mice. Confocal analysis of WAT from HF-fed, bone marrow-transplanted mice showed little evidence of significant APC differentiation into triglyceride-laden adipocytes, suggesting that conditions associated with nutrient excess may impair the ability of the adipose organ to store fat properly. Conclusions: These results demonstrate that putative APCs, and not EPCs, in epididymal WAT are derived from bone marrow. Furthermore, our data suggest that conditions of nutrient excess promote an imbalance in EPCs and APCs, the stoichiometry of which may be critical for the development of new adipocytes and for proper storage of fat.
Abstract 3083: Implantation of the Medtronic Melody Transcatheter Pulmonary Valve in Patients with Dysfunctional Right Ventricular Outflow Tract (RVOT) Conduits: Procedural and 6-Month Results from the U.S. Feasibility Trial
Authors: Doff B McElhinney, William E Hellenbrand, James E Lock, Thomas K Jones, John P Cheatham, Evan M Zahn
Journal: Circulation (2008)

RVOT conduit dysfunction can now be treated with transcatheter pulmonary valve placement (TPV). We report procedural and 6-mo outcomes of the prospective multicenter U.S. feasibility trial for TPV using the Melody valved stent. Outcomes included safety, procedural success and short-term effectiveness. Entry criteria included: weight ≥30 kg, RVOT conduit ≥16mm when implanted, and ≥moderate RVOT obstruction (RVOTO; mean gradient ≥40mmHg if NYHA class I, ≥35mmHg if higher) and/or regurgitation (PR; severe if class I, moderate if higher) by echo. Exercise testing, MRI, and chest CT were performed before catheterization (cath). The cath and TPV protocol was standardized, including high pressure balloon angioplasty when needed and balloon sizing of the conduit prior to TPV. In this 5-center study, 58 pts (median age 18 yrs) were enrolled and underwent cath from 1/07–5/08. The mean RVOT gradient on echo was 28±11mmHg and PR was severe or moderate in all but 5 pts. 5 pts were excluded at cath due to risk of coronary compression (2) or other predefined anatomic/hemodynamic factors (3). A Melody was implanted in the RVOT conduit in the other 53 pts. The peak RVOT gradient at cath was reduced from 36±16 to 15±7mmHg (p&lt;0.001) and all pts had no/trivial PR. There were no deaths. In 1 pt, TPV was complicated by conduit rupture, which was managed surgically. Non-surgical complications included atrial tachycardia (1) and PA perforation by a guidewire (1). In 30 pts with 6-mo follow-up data, PR was none (29) or trivial (1) by echo, and mean RVOT gradient was 20±8mmHg, unchanged from discharge (19±8mmHg, p=0.9 by paired analysis). On 6-mo MRI (n=26), median PR fraction was 0%, down from 29% pre-TPV (p&lt;0.001). 1 pt had a second Melody placed 3 mo after TPV for stent fracture and recurrent RVOTO. 8 other pts had stent fractures. There were no other adverse events through 6-mo follow-up, but 2 pts had a second Melody placed for stent fracture with RVOTO ~1 yr post-TPV. In this prospective multicenter study, TPV was safe and technically successful overall, with encouraging acute and 6-mo outcomes. Stent fracture with recurrent RVOTO, observed in ~10% of pts, may be overcome by pre-TPV RVOT angioplasty with or without stent implantation.
Out of hours services are not facing a crisis, minister says
Authors: Karen Hebert
Journal: BMJ (2004)

Changes to out of hours services should not cause a mass exodus of GPs from out of hours work. Nor should it effect emergency departments or care by GPs in normal hours, health minister John Hutton told MPs last week.

Mr Hutton was being questioned by the House of Commons Health Committee in the second session of its inquiry into GPs' out of hours services. Previous witnesses had expressed fears over the “potential crisis” in out of hours services when responsibility is transferred to primary care trusts ( BMJ 2004;329:13).

Responding to the …
Küçük Yassı Mide Kanseri Tanısında Yeni Odaklayıcı Endoskopi Tekniği: Bir Prospektif, Kör Çalışma
Authors: Kenshi Yao, Akinori Iwashita, Hiroshi Tanabe, Takashi Nagahama, Toshiyuki Matsui, Toshiharu Ueki, Suketo Sou, Yosuke Kikuchi, ve Makoto Yorioka
Journal: Clinical Gastroenterology and Hepatology (2007)
Qualitative accounts of living with cutaneous T-cell lymphoma
Authors: J. Barke
Journal: British Journal of Dermatology (2015)

Journal Article Qualitative accounts of living with cutaneous T‐cell lymphoma Get access J. Barke J. Barke University of the West of England Bristol BS16 1QY U.K Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 173, Issue 1, 1 July 2015, Pages 10–11, https://doi.org/10.1111/bjd.13841 Published: 01 July 2015
Abstract 13522: Vascular Healing in Drug-Eluting Stents: Differential Response of Limus-Eluting Stents in a Preclinical Model of Stent Implantation
Authors: Kazuko Tajiri, Akira Sato, Tomoya Hoshi, Taizo Kimura, Yoshihiro Seo, Satoshi Sakai, Michiaki Hiroe, Kazutaka Aonuma, Shigeyuki Watanabe
Journal: Circulation (2014)

Introduction: Recently, several clinical studies suggest that second-generation cobalt-chromium everolimus-eluting stents (CoCr-EES) lead to more favorable vascular healing than first-generation sirolimus-eluting stents (SES). However, it remains unclear the difference of vascular responses between CoCr-EES and SES. Methods: A total of 42 stents (14 bare-metal stents [BMS], 13 CoCr-EES, and 15 SES) were deployed in the iliac arteries of 31 Japanese White rabbits. Qquantitative real-time reverse-transcription polymerase chain reaction (QRT-PCR) analyses of the neointima were performed on days 30 and 90 after the stent implantation. Results: On day 30, the gene expression of the angiogenic growth factors (hepatocyte and basic fibroblast growth factors) and proteoglycans (decorin, biglycan and lumican) were more upregulated in the BMS than in the SES. However, at 90 days after stenting, the gene expression of proteoglycans and collagen was more upregulated in the SES than in the CoCr-EES and BMS. Conclusions: In contrast to CoCr-EES and BMS, SES strongly suppresses the vascular healing-related gene expression in the early phase, and then upregulates it in the late phase. These differences may lead to different clinical outcomes in humans.
Survival After Pancreas Transplantation—Reply
Authors: David M. Harlan, Maureen A. McBride
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
THE BLOOD SEDIMENTATION TEST: ITS USE AS A ROUTINE, ESPECIALLY IN PULMONARY TUBERCULOSIS
Authors: Paul H. Ringer, Mary Roach
Journal: Annals of Internal Medicine (1934)

Excerpt In 1918 Fåhraeus1 first noted an increase in the sedimentation rate of red cells in citrated blood in pregnant women. At once this was hailed as a new test for pregnancy; but it was soon found to be unreliable in the early months when, of course, its importance was greatest. The test is reliable for pregnancy after the twelfth week if no concurrent infection exists, but by that time its diagnostic value has necessarily been lost. Furthermore, it was soon discovered that acute and chronic infectious processes and malignant new growths had a marked effect upon the sedimentation rate. TECHNIC
International Collaborative Ovarian Neoplasm Trial 1 and Adjuvant ChemoTherapy In Ovarian Neoplasm Trial: Two Parallel Randomized Phase III Trials of Adjuvant Chemotherapy in Patients With Early-Stage Ovarian Carcinoma
Authors: J Baptist Trimbos, Mahesh Parmar, Ignace Vergote, David Guthrie, Giorgio Bolis, Nicoletta Colombo, Jan B Vermorken, Valter Torri, Constantino Mangioni, Sergio Pecorelli, Andrea Lissoni, Ann Marie Swart
Journal: JNCI Journal of the National Cancer Institute (2003)

Background: Adjuvant chemotherapy has been suggested as a possible strategy to improve survival in women with early-stage ovarian cancer; however, all randomized studies to date have been too small to answer this question reliably. Methods: We performed a preplanned combined analysis of two parallel randomized clinical trials (International Collaborative Ovarian Neoplasm 1 [ICON1] and Adjuvant ChemoTherapy In Ovarian Neoplasm [ACTION]) in early-stage ovarian cancer that compared platinum-based adjuvant chemotherapy with observation following surgery. Between November 1990 and January 2000, 925 patients (477 in ICON1 and 448 in ACTION) who had surgery for early-stage ovarian cancer were randomly assigned to receive platinum-based adjuvant chemotherapy (n = 465) or observation (n = 460) until chemotherapy was indicated. Kaplan–Meier analysis was used to compare overall and recurrence-free survival by treatment allocation. In subgroup analyses of pretreatment age, tumor stage, histologic cell type, and differentiation grade, the differences in relative size of effect were tested using a chi-square test for interaction or a chi-square test for trend. All tests of statistical significance were two-sided. Results: After a median follow-up of over 4 years, 245 patients had died or had a recurrence (ICON1: 133, ACTION: 112). Overall survival at 5 years was 82% in the chemotherapy arm and 74% in the observation arm (difference = 8% [95% confidence interval (CI) = 2% to 12%]; hazard ratio [HR] = 0.67, 95% CI = 0.50 to 0.90; P = .008). Recurrence-free survival at 5 years was also better in the adjuvant chemotherapy arm than it was in the observation arm (76% versus 65%, difference = 11% [95% CI = 5% to 16%]; HR = 0.64, 95% CI = 0.50 to 0.82; P = .001). Subgroup analyses provided no evidence of a difference in the size of effect of chemotherapy on survival in any pretreatment subcategory. Conclusions: Platinum-based adjuvant chemotherapy improved overall survival and recurrence-free survival at 5 years in this combined group of patients with early-stage ovarian cancer defined by the inclusion criteria of the ICON1 and ACTION trials.
Physician-Robot Makes the Rounds
Authors: Paul D Thacker
Journal: JAMA (2005)

SURGEON GARTH BALLANTYNE, MD, prefers to personally oversee his post-op patients. So when he had to travel to Europe last year, he turned on his laptop, hopped on the Internet, powered up a robot in Hackensack, NJ, and drove it into the recovery room. Soon, he was chatting with his patient, thousands of miles away. “Obviously, you have someone covering back home, but it’s nicer if you can keep in direct contact with the patients,” says Ballantyne, chief of minimally invasive surgery at the Hackensack University Medical Center. “And since the hospital has allelectronic records, not only can I see the patients but I can pull up the vital signs and results from blood tests and CT scans.”
Abstract 12446: Association of the Red Cell Distribution Width--A Component of the Intermountain Mortality Risk Scores--With In-Hospital Mortality in PCI Patients
Authors: Benjamin D Horne, Donald L Lappé, Raymond O McCubrey, Heidi T May, Stacey Knight, Viet Le, Tami L Bair, Joseph B Muhlestein, Jeffrey L Anderson
Journal: Circulation (2014)

Background: Post-hospitalization mortality is predicted by the red cell distribution width (RDW) in both CV and non-CV subjects. The Intermountain Mortality Risk Scores (IMRS) are sex-specific tools for 30-day mortality stratification composed of RDW and other Complete Blood Count (CBC) and/or basic metabolic profile (BMP) components. Whether RDW or IMRS predict in-hospital mortality is unknown.

Methods: Patients were included if treated by PCI at Intermountain Healthcare (males: N=6,007, females: N=2,165). In-hospital all-cause mortality was the primary endpoint (IMRS was derived for 30-day mortality). Secondary endpoints included hospital length of stay (LOS) and 30-day mortality. Logistic regression adjusted for age, in-hospital medications, HAS-BLED score, and (for RDW analyses) BMP and CBC components.

Results: The RDW predicted in-hospital mortality in both males and females (Table), and 30-day mortality in males (weakly in females). IMRS (using CBC only and CBC+BMP) strongly predicted in-hospital and 30-day mortality (Table). IMRS also predicted LOS for males (p<0.0001, β=0.299, CI=0.274, 0.323) and females (p=0.010, β=0.341, CI=0.081, 0.601).

Conclusions: RDW and IMRS predicted in-hospital mortality, even after multivariable adjustment. Early in-hospital use of IMRS may improve personalization of medical care through use of commonly-ordered, inexpensive, electronically-available admission labs. Whether use of IMRS improves in-hospital outcomes requires future randomized trials.

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Risk of intrapleural hemorrhage following thoracocenthesis
Authors: Marie-Ève Bureau, Yves Lacasse, Simon Martel
Journal: European Respiratory Journal (2012)

Background: Diagnostic or therapeutic thoracocenthesis is usually a simple and safe procedure. The extent to which coagulation abnormalities induced by antithrombotic therapy (ATT) increase the risk of of intrapleural hemorrhage following thoracocenthesis is unknown.  Objective: To examine the risk of intrapleural hemorrhage following diagnostic or therapeutic thoracocenthesis in patients with or without ATT.  Methods: We conducted a retrospective cohort study of all the diagnostic or therapeutic thoracocentheses performed in our institution over a three-year period (2007-2009). We considered only the procedures performed without ultrasonic guidance. Antiplatelets and anticoagulant therapy at the time of the procedure were recorded. Coagulation parameters (international normalized ratio, partial thromboplastin time, platelet count) and hemoglobin level were also noted. We defined intrapleural hemorrhage as either a recurrent pleural effusion with a fall in hemoglobin >20 grams/liter, or a hemothorax necessitating pleural drainage.  Results: A total of 695 patients underwent 955 thoracocentheses; data were available from 940 of them. 738 procedures (78.5%) were performed on patients on either antiplatelets or anticoagulant therapy. Overall, 7 intrapleural hemorrhages were noted (overall incidence rate: 0.7%), all in patients with antithrombotic therapy (ATT: 7/738 vs. no ATT: 0/202; Fisher9s exact test: p = 0.22).  Conclusion: The incidence of intrapleural hemorrhage following thoracocenthesis was low. Although the event rates in patients with or without ATT were not statistically different, all intrapleural hemorrhages occurred in patients with antithrombotic therapy.
Hydrogel Encapsulation of Cells in Core–Shell Microcapsules for Cell Delivery
Authors: Duy Khiem Nguyen, Young Min Son, Nae‐Eung Lee
Journal: Advanced Healthcare Materials (2015)

A newly designed 3D core-shell microcapsule structure composed of a cell-containing liquid core and an alginate hydrogel shell is fabricated using a coaxial dual-nozzle electrospinning system. Spherical alginate microcapsules are successfully generated with a core-shell structure and less than 300 μm in average diameter using this system. The thickness of the core and shell can be easily controlled by manipulating the core and shell flow rates. Cells encapsulated in core-shell microcapsules demonstrate better cell encapsulation and immune protection than those encapsulated in microbeads. The observation of a high percentage of live cells (≈80%) after encapsulation demonstrates that the voltage applied for generation of microcapsules does not significantly affect the viability of encapsulated cells. The viability of encapsulated cells does not change even after 3 d in culture, which suggests that the core-shell structure with culture medium in the core can maintain high cell survival by providing nutrients and oxygen to all cells. This newly designed core-shell structure can be extended to use in multifunctional platforms not only for delivery of cells but also for factor delivery, imaging, or diagnosis by loading other components in the core or shell.
Multi-centre evaluation of mass spectrometric identification of anaerobic bacteria using the VITEK® MS system
Authors: O. Garner, A. Mochon, J. Branda, C.-A. Burnham, M. Bythrow, M. Ferraro, C. Ginocchio, R. Jennemann, R. Manji, G.W. Procop, S. Richter, J. Rychert, L. Sercia, L. Westblade, M. Lewinski
Journal: Clinical Microbiology and Infection (2013)

Accurate and timely identification of anaerobic bacteria is critical to successful treatment. Classic phenotypic methods for identification require long turnaround times and can exhibit poor species level identification. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is an identification method that can provide rapid identification of anaerobes. We present a multi-centre study assessing the clinical performance of the VITEK(®) MS in the identification of anaerobic bacteria. Five different test sites analysed a collection of 651 unique anaerobic isolates comprising 11 different genera. Multiple species were included for several of the genera. Briefly, anaerobic isolates were applied directly to a well of a target plate. Matrix solution (α-cyano-4-hydroxycinnamic acid) was added and allowed to dry. Mass spectra results were generated with the VITEK(®) MS, and the comparative spectral analysis and organism identification were determined using the VITEK(®) MS database 2.0. Results were confirmed by 16S rRNA gene sequencing. Of the 651 isolates analysed, 91.2% (594/651) exhibited the correct species identification. An additional eight isolates were correctly identified to genus level, raising the rate of identification to 92.5%. Genus-level identification consisted of Actinomyces, Bacteroides and Prevotella species. Fusobacterium nucleatum, Actinomyces neuii and Bacteroides uniformis were notable for an increased percentage of no-identification results compared with the other anaerobes tested. VITEK(®) MS identification of clinically relevant anaerobes is highly accurate and represents a dramatic improvement over other phenotypic methods in accuracy and turnaround time.
Abstract P3-02-04: The contribution of the national health system to mammographic screening in Brazil
Authors: Ruffo Freitas-Junior, Danielle CN Rodrigues, Rosangela S Correa, João-Emilio Peixoto, Rosemar MS Rahal
Journal: Cancer Research (2015)

Abstract INTRODUCTION: In Brazil, access to mammography is provided through the National Health System (SUS), the Supplemental Health System, or is paid for directly by the patient. SUS is the official government system and was established to conform to the constitutional requirement that health is a right of all Brazilian citizens and a duty of the state. In recent years SUS breast cancer control policies have advanced and government strategies for early detection have strengthened. However, evaluations are needed to monitor the effectiveness of these actions. OBJECTIVE: To describe the coverage of mammography in breast cancer screening conducted by the National Health System in Brazilian macro-regions and states in 2012. METHODS: An ecological study, where the estimate of coverage was the number of exams performed expressed as a percentage of the number of exams expected in the target population of women 50-69 years old. The exams performed refer to target population mammography production data from the Outpatient Information System (CIS) of DATASUS. To calculate the expected number of exams for this population, the biennial screening recommendations of the National Cancer Institute (NCI) and an estimate of the female population based on the census of the Brazilian Institute of Geography and Statistics (IBGE) were used. RESULTS: The coverage estimate for SUS-performed mammography in Brazil in 2012 was 26.6%. Stratified by macro region, the lowest coverage was in the northern region (12.1%) and highest in the South (34.6%). National coverage of screening mammography in Brazil, performed by the National Health System (SUS), according to the geographical regions.Macro-regionsCoverageMinimumMaximump*North Region12.10.227.2Northeast Region23.910.337.20.001Southeast Region28.515.532.5South Region34.630.341.0Midwest Region14.810.319.7* Chi-square test Units of the Federation an estimated coverage ranged from 0.2% to 41%, the lowest in the state of Amapa and the largest in Santa Catarina. 48% of states were in the range of 10.1% to 20% coverage. CONCLUSION: The results imply that the contribution of SUS to mammography screening in Brazil is higher in both macro-regions and states with higher income and better organised health system. Citation Format: Ruffo Freitas-Junior, Danielle CN Rodrigues, Rosangela S Correa, João-Emilio Peixoto, Rosemar MS Rahal. The contribution of the national health system to mammographic screening in Brazil [abstract]. In: Proceedings of the Thirty-Seventh Annual CTRC-AACR San Antonio Breast Cancer Symposium: 2014 Dec 9-13; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2015;75(9 Suppl):Abstract nr P3-02-04.