tbuckley/cabot-search huggingface dataset:
453. Reproductive Safety Evaluation and Quantification of Adenoviral Vectors in the Mouse
Authors: Gyu Seek Rhee, Hyun Joo Lee, Soon Sun Kim, Seung Jun Kwack, Rhee Da Lee, Ji Hyun Seok, Soo Yeong Chae, Jae Woo Kim, Young Hyuk Won, Seung Hoon Lee, Kui Lea Park
Journal: Molecular Therapy (2004)

Expression of transgene other than in the target tissue may cause side effect and safety problems in gene therapy. This is particularly important if vector distributes to gonads, raising the possibility of inadvertent germ-line transmission. In addition, for indications such as prostate cancer and ovarian cancer, the proximity of the point of viral administration to organs of the reproductive system raises concerns regarding inadvertent germ-line transmission of genes carried by the virus. To evaluate the reproductive toxicity of in vivo adenovirus mediated gene transfer, we studied the biodistribution and potential germ-line transmission of p53-expressing adenovirus (Ad-CMV-p53). Both male and female Balb/c mice were injected with 1x 109PFU of Ad-CMV-LacZ or Ad-CMV-p53. DNA and RNA extracted from major organs including gonadal tissues were analyzed for vector sequences and expression. The PCR analysis showed that there were detectable vector sequences in liver, kidney, spleen, seminal vesicle, epididymis, prostate, ovary, and uterus. The RT-PCR analysis showed that Ad-CMV-LacZ or Ad-CMV-p53 viral RNA were present in spleen, prostate and ovary. Direct injected male and female mice of adenovirus vector into testis and ovary were mated and their offspring were evaluated for germ-line transmission of the adenoviral vector. The PCR analysis showed no evidence of germ-line transmission, although vector sequences were detected in DNA extracted from gonadal tissues. Quantitative PCR was used to determine adenovirus level over period of several weeks. Real-time PCR result confirmed a significant decrease of adenovirus in all tissues tested 1 week after injection. We have also developed for the cell specific localization of viral DNA in gonad tissues by using in-situ PCR. No positive signals were detected in germ cell related cells inside seminiferous tubules and follicles. These data provide strong evidence that the risk of the inadvertent germ-line transmission of vector sequences following intraperitoneal or direct injection into genito-urinary system of adenovirus is extremely low, although vector distributed to gonadal tissues.
Human Embryology and Morphology
Authors: Arthur Keith
Journal: JAMA (1949)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Addressing Overuse of Medical Services One Decision at a Time
Authors: Grace A. Lin, Rita F. Redberg
Journal: JAMA Internal Medicine (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Internal Medicine HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Internal Medicine journal
Photodynamic therapy and immunosuppression
Authors: S.H. Ibbotson
Journal: British Journal of Dermatology (2012)

Journal Article Photodynamic therapy and immunosuppression Get access S.H. Ibbotson S.H. Ibbotson Photobiology Unit, University Department of Dermatology, Ninewells Hospital and Medical School, Dundee DD1 9SY, U.K. E‐mail: s.h.ibbotson@dundee.ac.uk Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 167, Issue 3, 1 September 2012, Pages 465–467, https://doi.org/10.1111/j.1365-2133.2012.11189.x Published: 01 September 2012
NOLLE NOCERE THE RESPONSIBILITY OF THE PHARMACEUTICAL INDUSTRY
Authors: G E FARRAR
Journal: Annals of Internal Medicine (1959)

Editorial1 March 1959NOLLE NOCERE THE RESPONSIBILITY OF THE PHARMACEUTICAL INDUSTRYGEORGE E. FARRAR JR., M.D., F.A.C.P.GEORGE E. FARRAR JR., M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-50-3-829 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptNolle Nocere, primum nolle nocere: the first principle of therapeutics. "Do no harm"—literally, "To be unwilling to do harm." This is the principle on which rests public confidence in the physician.This first principle has stood firm throughout the centuries; only the connotation has changed. But this change, largely the result of the many outstanding advances in pharmacology which we have seen in our time, has been so great as to nearly obscure the principle. The lines of Pope, "Be not the first by whom the new are tried, nor yet the last to lay the old aside," are still...1 Dowling HF: Twixt the cup and the lip, J. A. M. A. 165: 657 (Oct. 12) 1957. CrossrefMedlineGoogle Scholar2 Sollman T: Manual of pharmacology, 7th Ed., 1948, W. B. Saunders Company, Philadelphia, p. 3. Google Scholar3 Editorial: Pitfalls of prepublication, Science 127: 623 (Mar. 21) 1958. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: *From a Morning Lecture presented at the Thirty-ninth Annual Session of The American College of Physicians, Atlantic City, New Jersey, April 30, 1958, by George E. Farrar, Jr., M.D., F.A.C.P., Associate Professor of Medicine, Temple University School of Medicine; Medical Director, Wyeth Laboratories, Philadelphia, Pennsylvania. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 March 1959Volume 50, Issue 3Page: 829-834KeywordsPharmacology ePublished: 1 December 2008 Issue Published: 1 March 1959 PDF downloadLoading ...
A potential role for α-actinin in inside-out αIIbβ3 signaling
Authors: Seiji Tadokoro, Tsuyoshi Nakazawa, Tsuyoshi Kamae, Kazunobu Kiyomizu, Hirokazu Kashiwagi, Shigenori Honda, Yuzuru Kanakura, Yoshiaki Tomiyama
Journal: Blood (2010)
Preliminary inferences on the age-specific seriousness of human disease caused by avian influenza A(H7N9) infections in China, March to April 2013
Authors: B J Cowling, G Freeman, J Y Wong, P Wu, Q Liao, E H Lau, J T Wu, R Fielding, G M Leung
Journal: Eurosurveillance (2013)

Between 31 March and 21 April 2013, 102 laboratory-confirmed influenza A(H7N9) infections have been reported in six provinces of China. Using survey data on age-specific rates of exposure to live poultry in China, we estimated that risk of serious illness after infection is 5.1 times higher in persons 65 years and older versus younger ages. Our results suggest that many unidentified mild influenza A(H7N9) infections may have occurred, with a lower bound of 210–550 infections to date.
Abstract 5709: The Pediatric Cardiac Quality of Life Inventory: A New Reliable and Valid Disease-specific Quality of Life Measure for Children and Adolescents with Congenital and Acquired Heart Disease
Authors: Bradley S Marino, Ryan Tomlinson, Gil Wernovsky, Dennis D Drotar, Kim W Hart, Elaine Buchakjian, Philip R Khoury, Aimee Baker, Kaleigh Coughlin, David Shera, Jane Newburger, Lynn Mahony, Kathleen Mussatto, Elizabeth Tong, Mitchell Cohen, Jo Wray, James W Gaynor, Mark A Helfaer, Anne E Kazak, Judy A Shea
Journal: Circulation (2008)

The Pediatric Cardiac Quality of Life Inventory (PCQLI) is a self-administered disease-specific quality of life (QOL) tool with patient and parent-proxy reporting (Child form 8 –12 yrs; Adolescent form 13–18 yrs), designed to assess the QOL of pediatric patients with congenital and acquired heart disease (CHD, AHD). Our purpose was to evaluate the reliability and validity of the PCQLI Seven centers recruited CHD and AHD patients age 8 –18 yrs. Patient-parent pairs completed the PCQLI, a generic QOL tool (PedsQL), and non-QOL questionnaires [Self Perception Profile for Children/Adolescents (SPPC/A); Youth Self Report and Child Behavioral Checklist (Achenbach)]. Test-retest reliability was evaluated by administering the PCQLI at 2 time points, and was assessed by Spearman correlation coefficient. Validity was tested by assessing: differences in PCQLI score among heart disease subgroups; associations between PCQLI score and the number of cardiac surgeries, catheterizations, hospitalizations, and doctor visits; the correlation between patient and parent PCQLI scores; and correlations between PCQLI score and the scores generated by generic QOL and non-QOL tools. The study was completed by 1486 patient-parent pairs. Heart disease subgroups included: biventricular repair (45.4%); Fontan palliation (12.6%); unrepaired CHD (12.0%); heart transplant (4.4%); and AHD (25.6%). Correlations evaluating test-retest reliability were high (r=0.78 – 0.90). Lower PCQLI score was associated with Fontan palliation (p&lt;0.001), as well as a greater number of cardiac surgeries, catheterizations, hospitalizations, and doctor visits (all p&lt;0.001). Disattenuated correlations between patient and parent PCQLI scores were moderate (r = 0.51– 0.69). The PCQLI score and generic QOL score were highly associated (r = 0.62– 0.77). Lower PCQLI score was strongly associated with lower Global Self-Worth score on the SPPC/A (p&lt;0.001), and lower Total Competency score (p&lt;0.001), higher Syndrome Scale scores, and higher DSM Oriented Scale scores on the Achenbach (p&lt;0.001). The PCQLI is a new disease-specific pediatric cardiac QOL tool that produces reliable and valid scores in children and adolescents age 8 –18 yrs. This research has received full or partial funding support from the American Heart Association, AHA Great Rivers Affiliate (Delaware, Kentucky, Ohio, Pennsylvania &amp; West Virginia).
Life long frustration: Tea for two and two for tea
Authors: L.-S. Teh
Journal: BMJ (1998)

Over 20 years ago, when I was reading Shakespeare's Romeo and Juliet in school, I did not realise that Juliet's lament, “What's in a name? that which we call a rose. By any other name would smell as sweet,” would also conjure up such bittersweet experiences for me.

I was green and timid when I entered Aberdeen University Medical School straight from cosmopolitan Malaysia, petrified that I would have difficulty following the lectures and understanding the patients. Scottish names were new to me—like an entirely different language. And as I stumbled over the pronunciation of the names of fellow classmates, they equally had difficulty over my Chinese name.

“I'm Lee-Suan Teh,” I volunteered again, without much hope that it would be remembered. “Yes, Suan, pronounced like swan and Teh, like the River Tay,” I …
The DST as a predictor of outcome in depression: a meta-analysis
Authors: Saulo C.M. Ribeiro, Rajiv Tandon, Leon Grunhaus, John F. Greden
Journal: American Journal of Psychiatry (1993)

OBJECTIVE: The authors undertook a review of the literature on the dexamethasone suppression test (DST) as a predictor of course and outcome in major depression, to illustrate clinical and pathophysiological implications of studying the relation between biological measures and course of illness in psychiatry. METHOD: In computerized searches and cross-references, 144 articles were found that related DST results to prediction of treatment response or outcome. Meta-analysis was performed on pooled data from all of the studies and separately on data from selected studies that had used stricter methodology. RESULTS: 1) Baseline DST status did not predict response to antidepressant treatment or outcome after hospital discharge. 2) Non-suppression of cortisol on the baseline DST predicted poorer response to placebo. 3) Persistent nonsuppression of cortisol on the DST after treatment was associated with high risk of early relapse and poor outcome after discharge. CONCLUSIONS: Baseline DST results may be devoid of prognostic value, but posttreatment nonsuppression of cortisol on the DST is strongly associated with poor outcome. Persistent nonsuppression may reflect a partially treated index episode or identify sicker patients who are unresponsive to treatment. The findings reiterate the importance of studying biological measures in relation to multiple validators of psychiatric illness beyond cross- sectional diagnosis.
Abstract MP72: Apolipoprotein B and Apolipoprotein B, LDL-C Discordance in Young Adults Are Associated With Coronary Artery Calcification at 25 Year Follow-Up: The Coronary Artery Disease in Young Adults (CARDIA) Study
Authors: Ron C Li, Cheeling Chan, Allan Sniderman, Kiang Liu, Donald Lloyd-Jones, John T Wilkins
Journal: Circulation (2014)

Introduction: High ApoB has been shown to predict cardiovascular disease (CVD) in adults even in the context of low LDL-C. It is not known, however, if high ApoB and high ApoB, low LDL-C discordance in young adults are associated with coronary artery calcium (CAC) in mid-life. Methods: Data were derived from CARDIA, a multicenter study of the development and determinants of CVD risk factors in young adults recruited at ages 18 to 30. All participants with complete baseline CVD risk factor data, ApoB, and year-25 CAC score were included in this study. Baseline lipid fractions and ApoB were measured by standard assays. Year-25 CAC was assessed using two consecutive CT scans with presence of CAC defined as having a positive, non-zero Agatston score using the average of two scans. Baseline ApoB values were divided into tertiles. Four mutually exclusive concordant/discordant groups were created based on median ApoB and LDL-C. Logistic regression was performed for unadjusted and adjusted models. Results: 3496 participants were included [mean age=25±3.6, BMI=24.5±5Kg/m2, 44.4% male, and the following mean lipid values (mg/dL): total cholesterol=177.3±33.1, LDL-C=109.9±31.1, HDL-C=53±12.8, ApoB=90.7±24, median triglycerides=61(IQR 46-83)]. Compared with the lowest ApoB tertile, the middle [OR=1.55 (95% CI 1.22-1.95)] and high [OR=2.35 (95% CI 1.87-2.97)] tertiles exhibited increased odds of developing year-25 CAC in traditional risk factor-adjusted models. High ApoB, low LDL-C discordance was also associated with year-25 CAC in adjusted models [OR=1.57 (95% CI 1.12-2.20)]. Conclusions: These data suggest a dose-response association between ApoB in young adults and presence of mid-life CAC independent of baseline traditional CVD risk factors. High ApoB, low LDL-C discordance was also associated with year-25 CAC, suggesting that ApoB in young adults may help identify individuals with modest LDL-C levels who are at increased risk for subclinical atherosclerosis in mid-life.
ACCF/AHA/AMA–PCPI 2011 Performance Measures for Adults With Coronary Artery Disease and Hypertension
Authors: Joseph Drozda, Joseph V Messer, John Spertus, Bruce Abramowitz, Karen Alexander, Craig T Beam, Robert O Bonow, Jill S Burkiewicz, Michael Crouch, David C Goff, Richard Hellman, Thomas James, Marjorie L King, Edison A Machado, Eduardo Ortiz, Michael O'Toole, Stephen D Persell, Jesse M Pines, Frank J Rybicki, Lawrence B Sadwin, Joanna D Sikkema, Peter K Smith, Patrick J Torcson, John B Wong
Journal: Journal of the American College of Cardiology (2011)
Inactivating mutations and overexpression of BCL10, a caspase recruitment domain-containing gene, in MALT lymphoma with t(1;14)(p22;q32)
Authors: Quangeng Zhang, Reiner Siebert, Minhong Yan, Bernd Hinzmann, Xiaoli Cui, Liquan Xue, Karen M. Rakestraw, Clayton W. Naeve, Georg Beckmann, Dennis D. Weisenburger, Warren G. Sanger, Hadwiga Nowotny, Michael Vesely, Evelyne Callet-Bauchu, Gilles Salles, Vishva M. Dixit, André Rosenthal, Brigitte Schlegelberger, Stephan W. Morris
Journal: Nature Genetics (1999)
Abstract 13799: Electrical Pacing Inhibits Gap Junction-Mediated Cardiac Cell -Cell Communication by Promoting Cx43-Acetylation
Authors: Valerio Azzimato, Viviana Meraviglia, Claudia Colussi, Maria Cristina Florio, Alice Panariti, Khaled Qanud, Carlo Gaetano, Peter P Pramstaller, Maurizio C Capogrossi, Giulio Pompilio, Fabio A Recchia, Ilaria Rivolta, Alessandra Rossini
Journal: Circulation (2014)

Background: Communication among cardiomyocytes depends upon Gap Junction (GJ) protein expression and conductance. Previous studies demonstrated that electrical stimulation can induce GJ remodeling and evidences from neurons also indicate that electrical pacing modifies Lysine acetylase (KAT) and deacetylases (KDAC) activities. Objectives: Aim of the present work was to establish whether electrical stimulation modulates GJ-mediated cardiac cell-cell communication by acetylation dependent mechanisms. Methods and Results: Neonatal rat cardiomyocytes (NRCM; n=3) and in HL-1 atrial cells (n=20) were exposed to electrical field stimulation for 24 hours (Ionoptix C-Pace®; 0.5 Hz, 20 V, 0.5 msec pulses). Connexin 43 (Cx43) expression decreased almost 50% in NRCM and 40 % in HL-1; in contrast Cx40 and Cx45 expression was unchanged. Further, confocal microscopy revealed that electrical stimulation induced Cx43 accumulation in the cytoplasm of HL-1 cells. Electrical stimulation significatly down-regulated KDAC activity up to the 30% (n=3), whereas KAT activity was not modified; the net effect was a general increase of cell protein acetylation, confirmed by western blot analysis. Specifically, the pacing-dependent acetylation of Cx43 was proven by immunoprecipitation assay (n=5). Interestingly, our model mimicked the action of the KDAC pan-inhibitors TSA and SAHA on Cx43 expression and intracellular distribution, although we did not observe Cx43 mRNA significant reduction in electrically stimulated cells. In agreement, MG132 proteasome inhibitor (10 μM) restored Cx43 expression level. Finally, also the treatment of paced cells with the KAT inhibitor Anacardic Acid (0.5 μM) was able to rescue Cx43 level (n=4). Intriguingly, preliminary results also indicate lateralization and increased acetylation of Cx43 in the left ventricles of dogs with pacing-induced dilated cardiomyopathy (n=2). Conclusions: In vitro electrical stimulation of cardiac cells promotes Cx43 acetylation, which results in Cx43 down-modulation and intracellular relocalization. These findings suggest that electrical activity-dependent increase in acetylation may represent a novel mechanism for the regulation of cardiomyocyte communication.
Safe and successful bone marrow biopsy: An anatomical and CT‐based cadaver study
Authors: Bhavana Konda, Swati Pathak, Inga Edwin, Priti Mishall, Sherry A. Downie, Todd R. Olson, Louis J. Reed, Ellen W. Friedman
Journal: American Journal of Hematology (2014)

Bone marrow biopsy is generally a safe procedure. However, infrequently the procedure is associated with serious injuries that are attributed to inadvertent needle penetration of the iliac bone's inner cortex. An evidence-based approach to needle orientation during iliac crest biopsy does not exist. In our study, the posterior to anterior path of the bone marrow needle from the posterior superior iliac spine (PSIS) was studied in human cadavers in two orientations: (1) perpendicularly to the coronal plane (the perpendicular approach) and (2) laterally toward the ipsilateral anterior superior iliac spine (ASIS) (the lateral approach). The biopsy needle was deliberately advanced through the inner ilial cortex in both approaches. Dissections and imaging studies were done to identify the relationship of the penetrating needle to internal structures. Both approaches begin with a perpendicular puncture of the outer cortex at the PSIS. The perpendicular approach proceeds anteriorly whereas in the lateral approach the needle is reoriented toward the ipsilateral ASIS before advancing. The lateral approach caused less damage to neurovascular structures and avoided the sacroiliac joint compared to the perpendicular approach. This procedure is best done in the lateral decubitus position. Proper use of the lateral approach should obviate many of the complications reported in the literature.
Differential Development of Systemic Lupus Erythematosus in NZM 2328 Mice Deficient in Discrete Pairs of BAFF Receptors
Authors: Chaim O. Jacob, Ning Yu, Vishal Sindhava, Michael P. Cancro, Rahul D. Pawar, Chaim Putterman, William Stohl
Journal: Arthritis & Rheumatology (2015)

To determine the development of systemic lupus erythematosus (SLE) in NZM 2328 (NZM) mice deficient in 2 BAFF receptors.NZM.BR-3(-/-) .BCMA(-/-) , NZM.BR-3(-/-) .TACI(-/-) , and NZM.BCMA(-/-) .TACI(-/-) mice were evaluated on the clinical, pathologic, serologic, and cellular levels. BAFF receptor expression and lymphocyte phenotype were assessed by flow cytometry, IgG-secreting cells by enzyme-linked immunospot assay, B cell responsiveness to BAFF and generation of Treg cells by in vitro culture, serum BAFF and total IgG and IgG autoantibody levels by enzyme-linked immunosorbent assay, renal immunopathology by immunofluorescence and histologic analyses, and clinical disease by assessment of proteinuria and mortality.Renal immunopathology and clinical disease were attenuated in NZM.BR-3(-/-) .BCMA(-/-) and NZM.BR-3(-/-) .TACI(-/-) mice but were accelerated in NZM.BCMA(-/-) .TACI(-/-) mice. Accelerated disease was associated with increases in B cells, IgG-secreting cells, serum autoantibody levels, and T cells (especially CD4+ activated memory cells), whereas attenuated disease was associated with reductions in many of these parameters. Serum BAFF levels were increased in all double-deficient NZM mice. Exogenous BAFF promoted the in vitro survival of B cells from NZM.BCMA(-/-) .TACI(-/-) or NZM wild-type mice but not those from NZM.BR-3(-/-) .BCMA(-/-) or NZM.BR-3(-/-) .TACI(-/-) mice. In vitro generation of Treg cells was reduced in NZM.BCMA(-/-) .TACI(-/-) mice, but not in NZM.BR-3(-/-) .BCMA(-/-) or NZM.BR-3(-/-) .TACI(-/-) mice.Elimination of B lymphocyte stimulator receptor 3 (BR-3) and TACI or BR-3 and BCMA inhibits the development of SLE in NZM mice. Selective targeting of BR-3 plus TACI or BR-3 plus BCMA may be an efficacious therapeutic approach in human SLE.