tbuckley/cabot-search huggingface dataset:
Protection against Mycobacterium tuberculosis infection by adoptive immunotherapy. Requirement for T cell-deficient recipients.
Authors: I M Orme, F M Collins
Journal: The Journal of Experimental Medicine (1983)

The results of this study demonstrate that spleen cells taken from mice at the height of the primary immune response to intravenous infection with Mycobacterium tuberculosis possess the capacity to transfer adoptive protection to M. tuberculosis-infected recipients, but only if these recipients are first rendered T cell-deficient, either by thymectomy and gamma irradiation, or by sublethal irradiation. A similar requirement was necessary to demonstrate the adoptive protection of the lungs after exposure to an acute aerosol-delivered M. tuberculosis infection. In both infectious models successful adoptive immunotherapy was shown to be mediated by T lymphocytes, which were acquired in the donor animals in response to the immunizing infection. It is proposed that the results of this study may serve as a basic model for the subsequent analysis of the nature of the T cell-mediated immune response to both systemic and aerogenic infections with M. tuberculosis.
Anti-neutrophil cytoplasmic antibodies in type 1 and 2 autoimmune hepatitis
Authors: D Zauli, S Ghetti, A Grassi, C Descovich, F Cassani, G Ballardini, L Muratori, F B Bianchi
Journal: Hepatology (1997)

Perinuclear anti-neutrophil cytoplasmic antibodies (pANCA) have been recently defined as the most sensitive autoantibody of type 1 autoimmune hepatitis (AIH-1). Their prevalence in type 2 autoimmune hepatitis (AIH-2) has not yet been evaluated. The aim of the present study was to verify the association of pANCA with AIH-1 in an Italian series and to investigate the prevalence of the antibodies in AIH-2 and in proper control groups represented by cases of chronic hepatitis C (CH-C) with similar autoimmune features. pANCA were found in 30 of 46 (65%) AIH-1 and in 4 of 30 (13%) ANA/smooth muscle antibody (SMA) positive CH-C ( P = .0000006). Nineteen AIH-2, 29 liver kidney microsomal antibody type 1/liver cytosol antibody type 1 (LKM1/LC1) positive CH-C cases and 50 healthy controls were all negative. In AIH- 1, pANCA were significantly ( P = .009) more frequent in males (8 of 9, 89%) than in females (22 of 37, 59%). All pANCA positive sera showed SMA of the antiactin type. The present data confirm that pANCA, although less prevalent in our series than in other reports, do associate with AIH-1 also in the Mediterranean area and show that it can identify a small subgroup (13%) of ANA/SMA positive chronic hepatitis C, in which autoimmune reactions might play a more relevant role than viral infection. They also show the antibodies are absent in AIH-2. In conclusion, pANCA appear to be mutually exclusive of LKM1 positivity, either hepatitis C virus-related or not, thus representing a further valuable tool to differentiate the two types of autoimmune hepatitis.
A piece of my mind. Connections
Authors: G. A. Thomas
Journal: JAMA (1991)

My baby is sick. I fall into a terror so disorienting that it feels like my old nightmare of stepping into a twilight world patterned like an abstract expressionist painting. Silence and sharp, angular structures are everywhere, hurting me when I try to find my way home. I cannot afford to take a deep breath, but I also cannot cry yet. It's very early in the morning—a Texas winter. The trees are dark and green against our bedroom windows; the air is cold and still. When I woke him for his bottle I still wanted to believe he only had viral gastroenteritis. In my arms he vomits propulsively, begins to wail, then abruptly falls silent. The physician inside this terrified mother notes in a flash of insight that I am observing the joint clinical manifestations of lethargy and irritability—remembering how strange I used to think it was that such polar
Intracranial Suppuration Secondary to Trauma
Authors: Donald M. Poretz
Journal: JAMA (1975)

INTRACRANIAL suppuration is a potential complication of infection caused by<i>Nocardia asteroides</i>. When the central nervous system is involved by this process, hematogenous dissemination from a pulmonary focus is assumed to have occurred, although this is not always possible to prove. Traumatic nocardial infection has been reported but is extremely unusual. This is a case report of a patient who sustained an injury to the head that eventually led to an infection caused by<i>Nocardia asteroides</i>. <h3>Report of a Case</h3> A 36-year-old, healthy construction worker was admitted to The Fairfax Hospital on Dec 26, 1972, because of an injury sustained at work when he was struck in the face by a metal wedge. He suffered a trimallar fracture on the left side and had violation of the left maxillary and ethmoidal sinuses. At surgery, it was noted that there was destruction to part of the left frontal lobe and that
The Reduced Expression of 6ckine in the <i>plt</i> Mouse Results from the Deletion of One of Two 6ckine Genes
Authors: Galya Vassileva, Hortensia Soto, Albert Zlotnik, Hideki Nakano, Terutaka Kakiuchi, Joseph A. Hedrick, Sergio A. Lira
Journal: The Journal of Experimental Medicine (1999)

6Ckine is an unusual chemokine capable of attracting naive T lymphocytes in vitro. It has been recently reported that lack of 6Ckine expression in lymphoid organs is a prominent characteristic of mice homozygous for the paucity of lymph node T cell (plt) mutation. These mice show reduced numbers of T cells in lymph nodes, Peyer's patches, and the white pulp of the spleen. The genetic reason for the lack of 6Ckine expression in the plt mouse, however, has remained unknown. Here we demonstrate that mouse 6Ckine is encoded by two genes, one of which is expressed in lymphoid organs and is deleted in plt mice. A second 6Ckine gene is intact and expressed in the plt mouse.
Phase I Clinical Trial of Autologous Ascites-derived Exosomes Combined With GM-CSF for Colorectal Cancer
Authors: Shengming Dai, Dong Wei, Zhen Wu, Xiangyang Zhou, Xiaomou Wei, Haixin Huang, Guisheng Li
Journal: Molecular Therapy (2008)

Exosomes are small membrane vesicles that are secreted by a multitude of cell types. The exosomes derived from dendritic cells (Dex), tumor cells (Tex), and malignant effusions demonstrate immunomodulatory functions, and are even under clinical trial for cancer treatments. In this study we report the phase I clinical trial of the ascites-derived exosomes (Aex) in combination with the granulocyte–macrophage colony-stimulating factor (GM-CSF) in the immunotherapy of colorectal cancer (CRC). The Aex isolated by sucrose/D2O density gradient ultracentrifugation are 60–90-nm vesicles that contain the diverse immunomodulatory markers of exosomes and tumor-associated carcinoembryonic antigen (CEA). Totally 40 patients (HLA-A0201+CEA+) with advanced CRC were enrolled in the study, and randomly assigned to treatments with Aex alone or Aex plus GM-CSF. Patients in both groups received a total of four subcutaneous immunizations at weekly intervals. We found that both therapies were safe and well tolerated, and that Aex plus GM-CSF but not Aex alone can induce beneficial tumor-specific antitumor cytotoxic T lymphocyte (CTL) response. Therefore, our study suggests that the immunotherapy of CRC with Aex in combination with GM-CSF is feasible and safe, and thus can serve as an alternative choice in the immunotherapy of advanced CRC. Exosomes are small membrane vesicles that are secreted by a multitude of cell types. The exosomes derived from dendritic cells (Dex), tumor cells (Tex), and malignant effusions demonstrate immunomodulatory functions, and are even under clinical trial for cancer treatments. In this study we report the phase I clinical trial of the ascites-derived exosomes (Aex) in combination with the granulocyte–macrophage colony-stimulating factor (GM-CSF) in the immunotherapy of colorectal cancer (CRC). The Aex isolated by sucrose/D2O density gradient ultracentrifugation are 60–90-nm vesicles that contain the diverse immunomodulatory markers of exosomes and tumor-associated carcinoembryonic antigen (CEA). Totally 40 patients (HLA-A0201+CEA+) with advanced CRC were enrolled in the study, and randomly assigned to treatments with Aex alone or Aex plus GM-CSF. Patients in both groups received a total of four subcutaneous immunizations at weekly intervals. We found that both therapies were safe and well tolerated, and that Aex plus GM-CSF but not Aex alone can induce beneficial tumor-specific antitumor cytotoxic T lymphocyte (CTL) response. Therefore, our study suggests that the immunotherapy of CRC with Aex in combination with GM-CSF is feasible and safe, and thus can serve as an alternative choice in the immunotherapy of advanced CRC.
Identification of HLA-DR–bound peptides presented by human bronchoalveolar lavage cells in sarcoidosis
Authors: Jan Wahlström, Jörn Dengjel, Bengt Persson, Hüseyin Duyar, Hans-Georg Rammensee, Stefan Stevanoviδc, Anders Eklund, Robert Weissert, Johan Grunewald
Journal: Journal of Clinical Investigation (2007)

Sarcoidosis is an inflammatory disease of unknown etiology, most commonly affecting the lungs. Activated CD4+ T cells accumulate in the lungs of individuals with sarcoidosis and are considered to be of central importance for inflammation. We have previously shown that Scandinavian sarcoidosis patients expressing the HLA-DR allele DRB1*0301 are characterized by large accumulations in the lungs of CD4+ T cells expressing the TCR AV2S3 gene segment. This association afforded us a unique opportunity to identify a sarcoidosis-specific antigen recognized by AV2S3+ T cells. To identify candidates for the postulated sarcoidosis-specific antigen, lung cells from 16 HLA-DRB1*0301pos patients were obtained by bronchoalveolar lavage. HLA-DR molecules were affinity purified and bound peptides acid eluted. Subsequently, peptides were separated by reversed-phase HPLC and analyzed by liquid chromatography-mass spectrometry. We identified 78 amino acid sequences from self proteins presented in the lungs of sarcoidosis patients, some of which were well-known autoantigens such as vimentin and ATP synthase. For the first time, to our knowledge, we have identified HLA-bound peptides presented in vivo during an inflammatory condition. This approach can be extended to characterize HLA-bound peptides in various autoimmune settings.
Alcohol and Drug Problems in Women
Authors: Margaret Bean
Journal: JAMA (1981)

<i>Alcohol and Drug Problems in Women</i>is an excellent, well-organized book. Ambitious in scope, it is impressive in its accomplishment, which is to assess and report the state of knowledge about a badly neglected area, to clarify directions for further work, and to pose questions about why some beliefs persist and some areas are never studied. The book consists of 17 chapters, each extensively referenced with a summary by different authors on different topics. But it is more than a collection of papers. Dr Kalant's introduction and overview ties together the different sections and gives the whole coherence. Cohesion is further reinforced by the selection of authors and the framework of critical vision of the editorial advisory board of the Addiction Research Foundation, Toronto. The central point of the book is that drug and alcohol use and abuse are different for women than for men, that women's drug and alcohol
Deriving the Genomic Tree of Life in the Presence of Horizontal Gene Transfer: Conditioned Reconstruction
Authors: James A. Lake, Maria C. Rivera
Journal: Molecular Biology and Evolution (2004)

The horizontal gene transfer (HGT) being inferred within prokaryotic genomes appears to be sufficiently massive that many scientists think it may have effectively obscured much of the history of life recorded in DNA. Here, we demonstrate that the tree of life can be reconstructed even in the presence of extensive HGT, provided the processes of genome evolution are properly modeled. We show that the dynamic deletions and insertions of genes that occur during genome evolution, including those introduced by HGT, may be modeled using techniques similar to those used to model nucleotide substitutions that occur during sequence evolution. In particular, we show that appropriately designed general Markov models are reasonable tools for reconstructing genome evolution. These studies indicate that, provided genomes contain sufficiently many genes and that the Markov assumptions are met, it is possible to reconstruct the tree of life. We also consider the fusion of genomes, a process not encountered in gene sequence evolution, and derive a method for the identification and reconstruction of genome fusion events. Genomic reconstructions of a well-defined classical four-genome problem, the root of the multicellular animals, show that the method, when used in conjunction with paralinear/logdet distances, performs remarkably well and is relatively unaffected by the recently discovered big genome artifact.
Different HLA associated gene combinations contribute to susceptibility for coeliac disease and dermatitis herpetiformis.
Authors: J A Sachs, J Awad, D McCloskey, C Navarrete, H Festenstein, E Elliot, J A Walker-Smith, C E Griffiths, J N Leonard, L Fry
Journal: Gut (1986)

Forty two white patients of British or Irish descent with coeliac disease and 28 with dermatitis herpetiformis were typed for class I HLA-A, B, and C, and class II DR and DQ antigens. In coeliac disease there was a significant increase in the frequencies of A1, B8, DR3, DR7, and DQw2 compared with controls but no increase of DR2. In dermatitis herpetiformis there were similarly increased frequencies of A1, B8, DR3, and DQw2. In contrast with coeliac disease, however, the frequency of DR7 (18%) was no different from the control group but there was an increased frequency of DR2.
Atropine Sulfate vs Atropine Methyl Bromide
Authors: Constante B. dePadua
Journal: JAMA (1969)

Effects of atropine sulfate and atropine methyl bromide on maternal and fetal heart rates were observed in 12 women at term. Both drugs were given in graded doses to a total dose of 0.8 mg/70 kg body weight. Both drugs caused significant acceleration of the mothers' heart rates, but only atropine sulfate accelerated fetal heart rates. We assume that atropine methyl bromide, because of its charge, did not penetrate the placental barrier as readily as atropine sulfate.
Abstract 3549: Synthetic triterpenoid CDDO-Me induces apoptosis in pancreatic cancer cells by inducing oxidative stress
Authors: Dorrah Deeb, Xiaohua Gao, Scott A. Dulchavsky, Subhash C. Gautam
Journal: Cancer Research (2011)

Abstract Pancreatic ductal adenocarcinoma (PDA) is a highly lethal human malignancy with dismal prognosis and few effective therapeutic options. Novel agents that are safe and effective are urgently needed. Oleanolic acid-derived synthetic triterpenoids are potent antitumorigenic agents, but their efficacy for pancreatic cancer has not been investigated. We have found that methyl-2-cyano-3,12-dioxooleana-1,9(11)-dien-28-oate (CDDO-Me), a oleanane-derived synthetic triterpenoid exhibits potent anticancer activity against human pancreatic cancer cell lines. CDDO-Me inhibited the growth of both K-ras mutated (MiaPaca2, Panc1 and Capan2) and wild-type K-ras (BxPC3) pancreatic cancer cells at very low concentrations. The growth inhibitory activity of CDDO-Me was due to the induction of apoptosis characterized by increased annexin-V-FITC and cleavage of PARP-1 and procaspases-3, -8 and-9. Killing of PDA cells by CCDO-Me involved the generation of reactive oxygen species (ROS) as determined from the oxidation of H2DCFD by flow cytometry and its inhibition by general purpose antioxidant NAC. CDDO-Me was also highly active against gemcitabine resistant MiaPaCa2 cells expressing markers of tumor-initiating cells (cancer stem-like cells?). An ongoing pilot experiment has shown the antitumor activity of CDDO-Me against BxPC3 xenograft in vivo. Together, these data demonstrated potential of CDDO-Me for treating pancreatic cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3549. doi:10.1158/1538-7445.AM2011-3549
USE OF BISHYDROXYCOUMARIN (DICUMAROL®) IN THE PRESENCE OF IMPAIRED RENAL FUNCTION
Authors: Julius J. Sachs
Journal: JAMA (1952)

The concept that impaired renal function is a contraindication to bishydroxycoumarin (dicumarol®) therapy had its origin in early animal experiments. Bollman and Preston 1 produced renal damage in dogs by subcutaneous injection of uranium acetate or by bilateral ligation of the ureters and found a marked sensitivity to bishydroxycoumarin. They noted a similar effect after bilateral nephrectomy. Richards and Steggerda 2 found that bilateral nephrectomy in rats greatly increased the effect of bishydroxycoumarin. An early clinical report by Allen, Barker, and Waugh 3 stated that the effect of bishydroxycoumarin on prothrombin time is excessive in cases of renal insufficiency and advised that the drug be used very cautiously or not at all in these patients. Allen, Barker, and Hines 4 cited cases of two patients treated with this drug, in whom suppression of urine developed owing to the accumulation of crystals of sulfonamide compounds in the renal pelvis. The prothrombin time, which was increased in association with the anuria, returned to normal when urine again was excreted by the kidneys.
Neonatal cases of infantile myofibromatosis do not derive from maternal cells transferred during pregnancy
Authors: P. Yousefi, K. Khosrotehrani, M. Oster, Y. de Prost, S. Fraitag, S. Aractingi
Journal: British Journal of Dermatology (2009)

Journal Article Neonatal cases of infantile myofibromatosis do not derive from maternal cells transferred during pregnancy Get access P. Yousefi, P. Yousefi UPMC Univ Paris 6, EA4053, Paris, FranceINSERM UMR_S893, Saint‐Antoine Research Center, Team 15, Paris, FranceDepartment of Dermatology, Assistance Publique‐Hôpitaux de Paris (AP‐HP), Hôpital Tenon, 4, rue de la Chine, 75020, Paris, France Search for other works by this author on: Oxford Academic Google Scholar K. Khosrotehrani, K. Khosrotehrani UPMC Univ Paris 6, EA4053, Paris, FranceINSERM UMR_S893, Saint‐Antoine Research Center, Team 15, Paris, FranceDepartment of Dermatology, Assistance Publique‐Hôpitaux de Paris (AP‐HP), Hôpital Tenon, 4, rue de la Chine, 75020, Paris, France Correspondence: Kiarash Khosrotehrani. E‐mail: kiarash.khosrotehrani@tnn.aphp.fr Search for other works by this author on: Oxford Academic Google Scholar M. Oster, M. Oster UPMC Univ Paris 6, EA4053, Paris, FranceINSERM UMR_S893, Saint‐Antoine Research Center, Team 15, Paris, FranceDepartment of Dermatology, Assistance Publique‐Hôpitaux de Paris (AP‐HP), Hôpital Tenon, 4, rue de la Chine, 75020, Paris, France Search for other works by this author on: Oxford Academic Google Scholar Y. De Prost, Y. De Prost Departments of Dermatology Search for other works by this author on: Oxford Academic Google Scholar S. Fraitag, S. Fraitag Pathology, AP‐HP, Hôpital Necker‐Enfants‐Malades, Paris, France Search for other works by this author on: Oxford Academic Google Scholar S. Aractingi S. Aractingi UPMC Univ Paris 6, EA4053, Paris, FranceINSERM UMR_S893, Saint‐Antoine Research Center, Team 15, Paris, FranceDepartment of Dermatology, Assistance Publique‐Hôpitaux de Paris (AP‐HP), Hôpital Tenon, 4, rue de la Chine, 75020, Paris, France Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 160, Issue 6, 1 June 2009, Pages 1356–1357, https://doi.org/10.1111/j.1365-2133.2009.09135.x Published: 01 June 2009
DOBUTAMINE STRESS ECHOCARDIOGRAPHY IN THE RISK STRATIFICATION OF PATIENTS WITH LOW AORTIC TRANSVALVULAR GRADIENT UNDERGOING TRANSCATHETER AORTIC VALVE REPLACEMENT: THE IMPACT OF CONTRACTILE RESERVE ON OUTCOMES
Authors: Salim Hayek, Mahmoud Abdou, Vasilis Babaliaros, Vinod Thourani, Emir Veledar, Sharon Howell, Stamatios Lerakis
Journal: Journal of the American College of Cardiology (2013)
524. Adenovirus-Mediated Gene Delivery of Decoy Death Receptors Protects Mice from Hepatitis
Authors: Delphyne Descamps, Frédéric Vigant, Stéphanie Esselin, Elisabeth Connault, Paule Opolon, Michel Perricaudet, Karim Benihoud
Journal: Molecular Therapy (2005)

Top of pageAbstract
Death receptors are involved in various diseases such as hepatitis, sepsis or graft rejection. Neutralizing antibodies against apoptotic ligands or soluble death receptors are currently used to inhibit apoptosis but side effects due to their systemic action can be encountered. Therefore, to specifically restrict the inhibition of death signaling to target cells, we have developped a strategy based on membrane-anchored death receptors lacking death domains (DD).

Using adenovirus-mediated gene delivery, we showed that mice expressing Fas decoy receptor (Fas|[Delta]|DD) in the liver survived to fulminant hepatitis induced by anti-Fas Jo2 challenge as efficiently as mice expressing a Fas soluble antagonist. This protection stems from an inhibition of apoptosis as documented by measurement of TUNEL-positive cells and PARP cleavage. Moreover, in a model of T cell-mediated (concanavalin A-induced) hepatitis, Fas|[Auml]|DD antagonist prevents hepatocytes damages and transaminases release. Finally, we showed that gene transfer by a recombinant adenovirus of a Tumor necrosis factor receptor 1 lacking the death domain (TNFR1|[Delta]|DD) efficiently protects mice from sepsis shock.

Such receptors lacking a death domain exert a dominant negative effect, avoiding a systemic inhibition of apoptosis. These inhibitors of apoptosis may be of therapeutical interest in different pathologies and they are currently being evaluated for their ability to protect cells transduced by adenovectors from destruction by the immune system.