tbuckley/cabot-search huggingface dataset:
Analysis of the glycoprotein and protein composition of Bernard-Soulier platelets by single and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Authors: A T Nurden, D Dupuis, T J Kunicki, J P Caen
Journal: Journal of Clinical Investigation (1981)

Previous reports have described conflicting results concerning the glycoprotein (GP) and protein composition of Bernard-Soulier platelets. In view of this controversy we have analyzed the platelets of four Bernard-Soulier patients using improved single and two-dimensional sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis procedures. An absence of staining for carbohydrate of membrane GP Ib was characteristic for the platelets of each patient. Major periodate-Schiff staining bands corresponding to membrane GP IIb, IIIa, and IIIb were clearly detected and their presence was confirmed by two-dimensional SDS-polyacrylamide gel electrophoresis. The protein content of the Bernard-Soulier platelets was increased two- to fourfold. However, analysis of their protein composition using 7-12% acrylamide gradient gels showed normal polypeptide profiles. Lactoperoxidase-catalyzed 125I-labeling of the Bernard-Soulier platelet surface proteins was followed by SDS-polyacrylamide gel electrophoresis and autoradiography. No labeling in the Ib position was detected whereas the other major membrane GP, including Ia and IIa, were normally located. In contrast, GP Ib was clearly detected by periodate-Schiff staining and autoradiography when normal human platelets that had been exhaustively treated with neuraminidase before the lactoperoxidase-catalyzed iodination were analysed. No abnormalities were detected in the GP patterns of membranes isolated from the patients' erythrocytes. Only a severe molecular abnormality or possible deletion of GP Ib could account for this major platelet lesion in the Bernard-Soulier syndrome.
Neuropsychiatry and Pituitary Disease: An Overview
Authors: Michael Weitzner
Journal: Psychotherapy and Psychosomatics (1998)

It has been recognized for some time that psychiatric symptoms, such as depression, anxiety, and behavioral alterations, may occur in patients who have pituitary disease. From other research focused on endocrine abnormalities seen in patients with psychiatric illness, it is understood that there is a significant interrelationship between the endocrine system and mental health. More recent research focusing on neural circuits in the brain and the impact of alterations in neurotransmission and neurohormonal modulation has shown that the prefrontal cortex can be affected by perturbations in functioning occurring in distant sites. Such is the situation with the hypothalamic-pituitary axis. Through its rich connections with other limbic structures, the hypothalamic-pituitary axis may affect the behavioral control exerted by the prefrontal cortex, causing mood and personality alterations. In the more severe cases, an apathy syndrome may develop which must be carefully differentiated from depression and other cognitive disorders. This report will review: (1) the neuroanatomical components that cause the behavioral changes observed in many patients with pituitary disease; (2) the current concept of apathy syndrome; (3) the differentiation of apathy syndrome from major depression; (4) the underlying neurobiology of apathy, and (5) potential treatments.
Effect of Cigarette Smoking on Coronary Blood Flow and Myocardial Metabolism
Authors: L. M. BARGERON, D. EHMKE, F. GONLUBOL, A. CASTELLANOS, A. SIEGEL, R. J. BING
Journal: Circulation (1957)

The effect of smoking on the heart has been a controversial subject. The present studies were undertaken to investigate the effect of cigarette smoking on the coronary flow, myocardial usage of oxygen and myocardial extraction of glucose, pyruvate, lactate, and ketones. Catheterization of the coronary sinus revealed that cigarette smoking in patients without heart disease results in a significant rise in coronary blood flow and heart rate and a significant decline in coronary vascular resistance and myocardial extraction of oxygen and glucose. No evidence of coronary vascular constriction was detected.
Arousal responses from apneic events during non-rapid-eye-movement sleep.
Authors: K Rees, D P Spence, J E Earis, P M Calverley
Journal: American Journal of Respiratory and Critical Care Medicine (1995)

Patients with obstructive sleep apnea (OSA) experience severe sleep disruption and consequent daytime sleepiness. Current arousal scoring criteria show that some obstructive apneic events do not end in a recognizable cortical electroencephalographic (EEG) arousal. It is not known whether events that end in an obvious EEG arousal differ from those that do not, in terms of EEG frequency changes during the apneic event, the respiratory effort developed prior to apnea termination, the degree of the postapneic increase in blood pressure, or changes in CO2 tensions. We studied 15 patients with OSA in early Stage 2 sleep and analyzed obstructive apneic events with and without typical EEG arousals, defining an arousal as a frequency shift to waking alpha rhythm of 1 s or longer. EEG signals were digitized and analyzed by fast Fourier transform during and immediately after each apnea. The median EEG frequency and mean pleural pressure of the first and second halves of the apneic episode were compared with that of the first breath. Peak pleural pressure was measured just before the end of the apneic episode. Systolic and diastolic blood pressures and CO2 tensions were measured at the onset and termination of apnea. For each patient, 10 events that ended in EEG arousal were compared with 10 events that did not. Mean apnea duration did not differ for the two groups of events. Median EEG frequency and pleural pressure increased significantly from 8.14 to 9.25 Hz and 15.4 to 22.1 cm H2O, respectively, as the apnea progressed, but there was no difference between the groups nor any difference in the peak pleural pressure.(ABSTRACT TRUNCATED AT 250 WORDS)
Response To Nasal Challenge With Lysine-aspirin In Subjects With Hypersensitivity Reactions To Nsaids With Respiratory Versus Cutaneous Involvement
Authors: M. Blanca, P. Campo, C. Rondon, J. Cornejo, I. Doña, L. Haggeman, M. Torres, L. Melendez, J. Rodriguez-Bada, G. Canto
Journal: Journal of Allergy and Clinical Immunology (2011)
Abstract 242: Dietary PUFA's alter the visceral adipose secretome, which modulates mammary stem cell self-renewal
Authors: Ray Esper, Michael Dame, Djuric Zora, William Smith, Wicha Max, Dean Brenner
Journal: Cancer Research (2014)

Abstract Rationale: Dietary PUFA's alter the expression and secretion of a variety of adipokines. Diets rich in omega-6 fatty acids shift the leptin:adiponectin ratio in favor of leptin, whereas diets rich in omega-3 fatty acids have the opposite effect. We tested the hypothesis that different ratios of adipose-derived factors differentially drive or inhibit normal mammary stem cell self-renewal. Methods: F344 rats were fed an obesogenic diet supplemented with arachidonic acid (AA) and eicosapentanoic acid (EPA) in either a 1:0 or 1:0.4 ratio. At 20 weeks, the animals were sacrificed and the visceral adipose explants were maintained in culture for up to 10 days. Conditioned media was collected at 48 hours. Adipokine concentrations were measured with the Proteome Profiler Adipokine Array and normalized to a leptin ELISA. Primary human mammary epithelial cells and the MCF10A breast cell line were expanded in a non-adherent sphere-forming assay as a measure of stem cell self-renewal. Cell suspensions were exposed to adipose conditioned media (1 mg/ml total protein concentration). After 10 days, spheres were counted and sphere size was measured using ImageJ. Results: Adipose explants were maintained in culture for up to 10 days with &amp;gt;99% viability. The secretome from the rats fed omega-6 PUFA's was enriched for leptin, resistin, serpin E1, and lipocalin-2. The omega-3 rich diet led to enrichment for adiponectin, pentraxin, and RBP4 in the adipose secretome. The adipose conditioned media from the omega-6 fed rats increased mammary stem cell self-renewal by 190%, whereas that from the rats fed omega-3 PUFA's attenuated this effect. Conclusion: The n3:n6 dietary ratio promotes a shift in the ratios of multiple secreted adipokines. These ratios have a differential effect on mammary stem cell self-renewal. A diet rich in omega-6 PUFA's leads to increased stem cell self-renewal, whereas a diet supplemented with EPA attenuated this effect. Further studies will clarify the role of specific adipokines on mammary stem cell self-renewal. Selected adipokine levels in adipose conditioned mediaOmega-3 rich dietOmega-6 rich dietn6:n3 dietary ratio1:0.41:0.0Adiponectin1864.7778.3Leptin181.2208.7Lipocalin-244.1323.1Pentraxin-277.00.8Pentraxin-319.29.3Pref-122.98.8Resistin737.01372.3Serpin E125.877.4RBP41136.0318.1 Citation Format: Ray Esper, Michael Dame, Djuric Zora, William Smith, Wicha Max, Dean Brenner. Dietary PUFA's alter the visceral adipose secretome, which modulates mammary stem cell self-renewal. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 242. doi:10.1158/1538-7445.AM2014-242
Shire Hall Communications and the case for hepatitis B immunisation
Authors: D. Carnall
Journal: BMJ (1996)

Thank you for your interest in spreading the word about The BMJ.NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address. Username * Your Email * Send To * You are going to email the following Shire Hall Communications and the case for hepatitis B immunisation Your Personal Message CAPTCHA This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Pay-for-Performance and Accountability
Authors: Christine A. Sinsky
Journal: Annals of Internal Medicine (2007)

Letters5 June 2007Pay-for-Performance and AccountabilityChristine A. Sinsky, MDChristine A. Sinsky, MDFrom Medical Associates Clinic and Health Plan, Dubuque, IA 52001.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-146-11-200706050-00019 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:In his article, Rowe (1) makes several important points about value-based purchasing, especially the need to develop meaningful measures for complex patients and the tension between rewarding the absolute level of achievement versus the degree of improvement. Four assumptions in the paper, however, deserve comment: the assumptions that health information technology is the cure for quality, that extra patients aren't really extra work, that primary care physicians are not specialists, and that performance measurement should be focused at the physician level.First, the author refers to the “obvious advantage” of an electronic health record (EHR) in achieving ...Reference1. Rowe JW. Pay-for-performance and accountability: related themes in improving health care. Ann Intern Med. 2006;145:695-9. [PMID: 17088584] LinkGoogle Scholar Author, Article, and Disclosure InformationAuthors: Christine A. Sinsky, MDAffiliations: From Medical Associates Clinic and Health Plan, Dubuque, IA 52001.Disclosures: None disclosed. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoPay-for-Performance and Accountability: Related Themes in Improving Health Care John W. Rowe Pay-for-Performance and Accountability Mahesh Krishnamurthy Pay-for-Performance and Accountability Marcy Zwelling Pay-for-Performance and Accountability John W. Rowe Pay-for-Performance and Accountability Christine K. Cassel Metrics 5 June 2007Volume 146, Issue 11Page: 823KeywordsComputersConflicts of interestElectronic medical recordsHealth careHealth information technologyHospitalsOphthalmologyPrimary care physiciansVeins ePublished: 5 June 2007 Issue Published: 5 June 2007 Copyright & PermissionsCopyright © 2007 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Atlas-based whole-body segmentation of mice from low-contrast Micro-CT data
Authors: Martin Baiker, Julien Milles, Jouke Dijkstra, Tobias D. Henning, Axel W. Weber, Ivo Que, Eric L. Kaijzel, Clemens W.G.M. Löwik, Johan H.C. Reiber, Boudewijn P.F. Lelieveldt
Journal: Medical Image Analysis (2010)
Gastric Hypersecretion, Peptic Ulceration and Islet-Cell Tumor of the Pancreas (the Zollinger–Ellison Syndrome)
Authors: Robert M. Donaldson, Paul R. vom Eigen, Richard W. Dwight
Journal: New England Journal of Medicine (1957)

THE association of noninsulin-producing islet-cell tumors of the pancreas and progressive peptic ulceration, recorded from time to time in the medical literature, until recently was thought to be coincidental. Zollinger and Ellison1 described 2 patients with such findings, and reviewed 4 other cases collected from the literature. They suggested that there might be an etiologic relation between the islet-cell tumors and the occurrence of peptic ulcer. More recently Ellison2 collected 24 cases from various sources and postulated the existence of a clinical entity consisting of gastric hypersecretion and hyperacidity, severe, recurrent and often atypically located peptic ulceration, and the presence . . .
Using rapid appraisal to assess need Inappropriate in inner cities
Authors: T Hares, K Finch, P Naish
Journal: BMJ (1994)

EDITOR,- The endeavour to link community perceptions of need to local provision of services is laudable, but there are difficulties with the general applicability of the modified rapid appraisal technique outlined by S A Murray and colleagues.1 The practice based model described was used in an estate with a homogeneous, stable community, half of which was served by a single general practice. In our experience of needs assessment in two small inner city wards in the west end of Newcastle we have encountered highly mobile populations (21% of residents had moved house in the previous year)2 and diverse ethnic populations served by 16 general practices.

Given …
Is the USA's problem ours too?
Authors: M. McCartney
Journal: BMJ (2012)

The United Kingdom is fortunate that it is insulated against some of the needless overtesting, overtreating, and overdiagnosing that doctors in the United States are rising up against. Instead, we have the National Institute for Health and Clinical Excellence (NICE), which offers evidence based appraisals of healthcare interventions and screening and which protects against treatments of little or no efficacy being offered on the NHS. We also have the UK National Screening Committee, which provides critical reviews and recommendations of what screening is effective and useful.

Does this mean that we can relax? Unfortunately not. The general practice contract has improved the quantification of what we do, but the price of this is that more patients are exposed to guidelines rich in pharmaceutical recommendations. Some may be beneficial. However, most lack guidance about when …
Mutations causing medullary cystic kidney disease type 1 lie in a large VNTR in MUC1 missed by massively parallel sequencing
Authors: Andrew Kirby, Andreas Gnirke, David B Jaffe, Veronika Barešová, Nathalie Pochet, Brendan Blumenstiel, Chun Ye, Daniel Aird, Christine Stevens, James T Robinson, Moran N Cabili, Irit Gat-Viks, Edward Kelliher, Riza Daza, Matthew DeFelice, Helena Hůlková, Jana Sovová, Petr Vylet'al, Corinne Antignac, Mitchell Guttman, Robert E Handsaker, Danielle Perrin, Scott Steelman, Snaevar Sigurdsson, Steven J Scheinman, Carrie Sougnez, Kristian Cibulskis, Melissa Parkin, Todd Green, Elizabeth Rossin, Michael C Zody, Ramnik J Xavier, Martin R Pollak, Seth L Alper, Kerstin Lindblad-Toh, Stacey Gabriel, P Suzanne Hart, Aviv Regev, Chad Nusbaum, Stanislav Kmoch, Anthony J Bleyer, Eric S Lander, Mark J Daly
Journal: Nature Genetics (2013)
Thomas Leopold Venables
Authors: B. Holmes
Journal: BMJ (2013)

Thomas Leopold Venables (“Tom”) was born in Manchester, and went to Cambridge to read medicine, taking up his place in 1958 after national service in the Royal Air Force. After Westminster Hospital he returned to the RAF, and obtained his MRCP.

He came to Nottingham in 1970, where he practised until retirement. He was held in enormous regard by his patients, many of whom continued to …
Gluten sensitivity: real or not?
Authors: F. Godlee
Journal: BMJ (2012)

Does non-coeliac gluten sensitivity exist? The author of this week’s Patient Journey has no doubt of it (doi:10.1136/bmj.e7982). After years of unexplained ill health, a chance conversation on an internet forum led him to try an exclusion diet. The results were dramatic, he says. “Within a week of excluding gluten and lactose from my diet, all my symptoms had dramatically improved in just the same way as when I previously starved myself.” Accidental rechallenge brought the symptoms back “within hours.”

Despite symptomatic relief he wanted a “proper diagnosis.” He saw Kamran Rostami, whose account of the condition accompanies the patient’s story. “Patients have negative immunoallergy tests to wheat and negative coeliac …
Book: Safe Food: Bacteria, Biotechnology and Bioterrorism
Authors: Richard Mayon-White
Journal: BMJ (2003)

There is much distrust of both the food industry and the ability of government agencies to protect the public from unsafe food. The current debate over genetically modified (GM) crops is a good example of the concern. One of the reasons for the distrust is that scientific risk assessments do not convince the many people who make value based judgments on the safety of consumer products. Another factor is that safety, whether of food or another product or service, has a political dimension that cannot be ignored, a theme throughout Safe Food and a help in understanding people's concerns.​concerns.




Figure 1



Marion Nestle

University of California Press, £19.95/$27.50, pp 356 ISBN 0 520 23292 5 www.ucpress.edu

Rating: ★★★




There are interesting transatlantic similarities and differences in food safety. In both the United States and the United Kingdom, there have been scares caused by salmonella and Escherichia coli O157 food poisoning. In both countries, the agencies responsible for food safety have been perceived to put the interests of food producers ahead of public health. There have been calls for a single organisation that has overall responsibility for food safety in the United States, something that the United Kingdom has gained from the Food Standards Agency. A difference is that the American public has been much more accepting of GM crops than Europeans.

True stories about outbreaks and legal actions can be exciting as fiction. In this book, the stories enlighten the more analytical sections, which are scholarly. The episodes of food contamination in the United States reveal the complex links in the food and pharmaceutical industries, and the diffusion of responsibility that followed takeovers, mergers, and sales of parts of companies with their patents. Matching this scattering of accountability is the labyrinth of American government supervision and control. An impression left by the ineffectiveness of government agencies is that consumer organisations must exercise their powers if food is to be kept safe.

A stimulus for the book was the terrorist destruction of the World Trade Center on 11 September 2001. This brings the discussion back from consumerism to the importance of the proper governmental organisations to monitor food safety and to respond to suspected contamination, whether accidental or deliberate. The book concludes with a clear statement of what needs to be done to ensure safe food, listing actions to be taken by the food industry, by the US federal government, and the public (for example, GM foods should be labelled in America, just as they have to be in Europe). This book is a good read for anyone trying to decide about GM food.
Delay to invasive investigation and revascularisation for coronary heart disease in south west Thames region: a two tier system?
Authors: M Marber, C MacRae, M Joy
Journal: BMJ (1991)

OBJECTIVES--To assess the accessibility of invasive investigation and revascularisation procedures in the management of coronary heart disease in a defined population in the South West Thames region over 10 years, and to audit the performance of both the NHS and the private sector. DESIGN--Analysis of all patient referrals to the regional cardiothoracic centres for coronary heart disease during 1979-88. SETTING--North West Surrey District Health Authority, which had a mean catchment population of 205,000 during the study period. The health district is one of the 13 in the South West Thames region. PATIENTS--823 patients aged 34-80 years with suspected coronary heart disease, 204 of whom were referred for private investigation and 619 were referred within the NHS. The NHS referrals were mainly to St Thomas9s Hospital during 1979-83 and to St George9s Hospital during 1984-8. MAIN OUTCOME MEASURES--Difference in time to investigation and intervention between the NHS and the private patients. RESULTS--After some variation in earlier years the mean (SD, range) waiting times from referral to cardiac catheterisation and then revascularisation increased progressively in NHS patients, to 115.8 (126.5, 22-482) days and 305.9 (164.4, 22-620) days respectively in 1988. There was no significant change over the 10 years in waiting times within the private sector, with a mean of 17.2 (18.2, 1-62) days to angiography and 22.8 (14.5, 2-152) days to surgery. Fifteen people on the NHS waiting list died of probable cardiac causes. No people on the private waiting list died. CONCLUSIONS--The performance of the NHS system in South West Thames region in response to emergency referral is adequate. The waiting time to routine investigation and revascularisation is prolonged and seems to be worsening despite increased investigative and surgical activity by the regional centre. The delays may subject NHS patients to unnecessary risk, which is not shared by private patients.
Oxidative stress induces the endoplasmic reticulum stress and facilitates inclusion formation in cultured cells
Authors: Shinichiro Hanada, Masaru Harada, Hiroto Kumemura, M. Bishr Omary, Hironori Koga, Takumi Kawaguchi, Eitaro Taniguchi, Takafumi Yoshida, Takao Hisamoto, Chikatoshi Yanagimoto, Michiko Maeyama, Takato Ueno, Michio Sata
Journal: Journal of Hepatology (2007)
Pheochromocytoma. Treatment with alpha- and beta-adrenergic blocking drugs
Authors: R. M. Crago
Journal: JAMA (1967)

A 21-year-old Negro woman with a suprarenal pheochromocytoma on the left side was treated preoperatively with orally administered phenoxybenzamine hydrochloride and propranolol hydrochloride which are, respectively, α- and β-adrenergic blocking agents. The patient had had hypertension for six months prior to admission. Findings from physical examination were not remarkable except that blood pressure when the patient was supine was 180/120 mm Hg and the cardiac rate average 85 beats per minute. A 47 gm tumor was removed from the left suprarenal area at operation. Throughout the operation the cardiac rate generally ranged from 80 to 90 beats per minute. No significant arrhythmias occurred at any time. We suggest that the patient was protected from β-adrenergic mediated cardiac arrhythmias with the use of propranolol. Phenoxybenzamine and propranolol should be given further evaluation in the preoperative treatment of patients with a functioning phenochromocytoma.