tbuckley/cabot-search huggingface dataset:
Abstract 12531: Post-Discharge Statin Withdrawal Had a Deleterious Impact on Long-Term Clinical Outcomes in Patients With Acute Myocardial Infarction
Authors: Min Chul Kim, Hae Chang Jeong, Keun Ho Park, Doo Sun Sim, Young Joon Hong, Ju Han Kim, Myung Ho Jeong, Jeong Gwan Cho, Jong Chun Park, Youngkeun Ahn
Journal: Circulation (2014)

Introduction: Many patients discontinue statin after acute myocardial infarction (AMI) despite its necessity. However, limited data are available describing the clinical impact of statin withdrawal after AMI. Hypothesis:The aim of this study was to evaluate the clinical outcomes of statin withdrawal in surviving patients after acute myocardial infarction (AMI). Methods: A total of 3807 patients in the COnvergent REgistry of cAtholic and chonnAm university for Acute MI (COREA-AMI) from 2004 to 2009 who survived for 1 year after AMI were enrolled. All patients were prescribed statin at discharge and were divided into two groups on the basis of statin withdrawal history: 603 patients had a history of statin discontinuation and 3204 patients continued statin therapy for 1 year after AMI. The primary outcome was mortality from any cause. We also analyzed the incidence of cardiac death, nonfatal MI, any revascularization and stroke from 1 year after AMI. The duration of follow-up was 4 years after AMI. Results:...
Transmural Migration of Intestinal Bacteria
Authors: Fritz B. Schweinburg, Arnold M. Seligman, Jacob Fine
Journal: New England Journal of Medicine (1950)

IN UREMIC patients subjected to peritoneal dialysis with a slightly hypertonic fluid1 peritonitis due to Escherichia coli developed frequently in spite of meticulous sterile technic. The evidence suggested that bacteria entered the peritoneal cavity by transmural migration.1 Preliminary experiments designed to test this assumption showed that Esch. coli can be recovered in small numbers from the peritoneal cavity of normal dogs after continued intraperitoneal injection of sterile irritating agents such as Monolate or gum tragacanth.2 In uremic dogs, prolonged exposure of the peritoneum to dialyzing fluid was followed by the appearance of Esch. coli in pure culture or as the . . .
Drug points: Angio-oedema associated with risperidone
Authors: C Cooney, A Nagy
Journal: BMJ (1995)

Drs C COONEY and A NAGY (Psychiatric Unit, Barnet General Hospital, Barnet, Hertfordshire EN5 5QD) write: A 30 year old woman with a history of schizoaffective disorder was admitted to this psychiatric unit. She had been treated with a combination of intramuscular depot flupenthixol and oral thioridazine, and paroxetine treatment had been started two months previously. On admission she was receiving thioridazine 100 mg nightly and paroxetine …
<i>In vitro</i> Characterization of Synthesized Thyroglobulin Immune Complexes (IC)
Authors: Ulla Feldt‐Rasmussen, Steffen Husby, Ole Blärbjerg, John Date, Henrik Nielsen
Journal: Allergy (1981)

Human thyroglobulin (Tg) immune complexes (TgIC) were preformed at different ratios from purified Tg, and Tg antibodies (TgAb) obtained from six patients with autoimmune thyroid diseases. TgIC were characterized by precipitation with polyethylene glycol, and sedimentation in sucrose gradient. TgIC from one patient were further characterized by concanavalin A (con A), and binding to the complement factor Clq and rheumatoid factor (RF). Tg and TgIC had almost identical binding to con A, no binding to Clq, but could be partially separated by ultracentrifugation and polyethylene glycol. The highest degree of separation was obtained by a RF‐coated plastic tube radiometric assay, using 125‐I‐rabbit TgAb as indicator. Tg showed no binding to RF, and a dose‐response curve of TgIC in serum could be established. There was a dependence of the Tg‐TgAb ratio, TgIC at equilibrium and in antibody excess being detected most efficiently. The method may serve as an aid in the evaluation of the fate of Tg, TgAb and TgIC following thyroid damage (surgery, radioiodine) and may be extended as a model system in the investigation of immune complexes in connection with autoimmune disorders.
Teledermatology applied following patient selection by general practitioners in daily practice improves efficiency and quality of care at lower cost
Authors: J.P. van der Heijden, N.F. de Keizer, J.D. Bos, P.I. Spuls, L. Witkamp
Journal: British Journal of Dermatology (2011)

Summary Background Teledermatology, the application of telemedicine in the field of dermatology, has similar accuracy and reliability as physical dermatology. Teledermatology has been widely used in daily practice in the Netherlands since 2005 and is fully reimbursed. Objectives This study prospectively investigated the effect of teledermatology on efficiency, quality and costs of care when integrated in daily practice and applied following patient selection by the general practitioner (GP). Methods Teledermatology consultations between GP and regional dermatologist were performed in daily GP practice in the Netherlands. Efficiency of care was measured by the decrease in the number of physical referrals to the dermatologist. Quality of care was measured by the percentage of teleconsultations for second opinion, physical referrals resulting from these teleconsultations, the response time of the dermatologists and educational effect experienced by the GP. Costs of conventional healthcare without teledermatology were compared with costs with teledermatology. Results One thousand, eight hundred and twenty GPs and 166 dermatologists performed teledermatology, and 37 207 teleconsultations performed from March 2007 to September 2010 were included. In the group of patients where the GP used teleconsultation to prevent a referral (n =26 596), 74% of physical referrals were prevented. In the group of patients where the GP used teleconsultation for a second opinion (n =10 611), 16% were physically referred after teleconsultation. The prevented referral rate in the total population was 68%. The mean response time of dermatologists was 4·6 h (median 2·0). GPs indicated that there was a beneficial educational effect in 85% of the teleconsultations. The estimated cost reduction was 18%. Conclusions Teledermatology can lead to efficient care probably at lower cost. We are therefore of the opinion that teledermatology following GP selection should be considered as a possible pathway of referral to secondary care.
Abstract 18592: Anti-arrhythmic Effect of a Novel Rycal, S44121 / Arm036, in a Post-myocardial Infarction Mouse Model of Heart Failure
Authors: Jerome Thireau, Charlotte Farah, Muriel Bouly, Jerome Roussel, Alain Lacampagne, Sylvain Richard
Journal: Circulation (2014)

Introduction: Targeting leaky cardiac ryanodine receptors (RyR2) to prevent diastolic Ca2+ release from the sarcoplasmic reticulum (SR) is a promising pharmacological approach, to rescue the impaired cardiac contraction and prevent Ca2+-dependent arrhythmias in heart failure (HF) and disease. Hypothesis: Based on prior work from the Marks group, the Rycal S44121 (also known as ARM036) is an experimental small molecule stabilizer of RyR. We investigated the effects of S44121 in a post-myocardial infarction (PMI) mouse model of HF. Methods and results: Mice were randomly assigned to 3 groups: Sham, PMI (subjected to left coronary artery ligation), and PMI-S (treated for 3 weeks with S44121 by subcutaneous osmotic pumps on day 7 post-MI, 10 mg/kg/day). Intracellular Ca2+ was measured on single left ventricular myocytes. PMI mice exhibited a 4-fold increase in the frequency of spontaneous Ca2+ release events, Ca2+ sparks, as measured in quiescent cells using the fluorescent Ca2+ indicator Fluo-4. PMI mice also exhibited higher global diastolic Ca2+, measured with the ratiometric fluorescent probe, Indo-1 AM, and increased the occurrence of ectopic diastolic Ca2+ waves. Acute application of S44121 (10 μM for 15 min) reduced Ca2+ sparks frequency. Chronic treatment of mice with S44121 also normalized the frequency of Ca2+ sparks and of ectopic Ca2+ waves, and corrected diastolic cellular Ca2+ overload. Effects were maximal at 20 mg/kg/day. Furthermore, treatment with S44121 abolished Ca2+ waves promoted by β-adrenergic challenge (acute application of isoproterenol, 10 nM). The potential anti-arrhythmic benefit of S44121 was assessed in vivo using telemetric surface electrocardiograms. S44121 had no effect on ECG intervals and did not alter the heart rate. However, anti-arrhythmic effects were confirmed by observation of a dose-dependent reduction of spontaneous ventricular extrasystoles and ventricular tachycardia. Near maximum benefits were observed at 10 mg/kg/day, both in basal conditions or following a challenge with acute treatment of isoproterenol (0.5 mg/kg, dosed ip). Conclusion: In mice with post-ischemic HF, treatment with S44121 prevented the abnormal diastolic SR Ca2+ leak and ectopic Ca2+ waves, and reduced ventricular arrhythmias.
Effect of increasing pump speed during exercise on peak oxygen uptake in heart failure patients supported with a continuous‐flow left ventricular assist device. A double‐blind randomized study
Authors: Mette Holme Jung, Peter Bo Hansen, Kaare Sander, Peter Skov Olsen, Kasper Rossing, Soeren Boesgaard, Stuart D. Russell, Finn Gustafsson
Journal: European Journal of Heart Failure (2014)
Expression of α-defensin 1-3 in T cells from severe cutaneous drug-induced hypersensitivity reactions
Authors: E. Morel, L. Álvarez, R. Cabañas, A. Fiandor, R. Díaz, S. Escamochero, N. Prior, M. Blanca, T. Bellón
Journal: Allergy (2010)

Cytotoxic T cells seem to be the main effector cells in Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN). However, recent data support a role of the innate immune system in the etiopathology of drug-induced cutaneous reactions. In this study, we analyzed the expression of α-defensins 1-3 in mononuclear cells from patients with SJS/TEN, drug-induced maculopapular exanthema (MPE), and healthy donors.DEFA1A3 gene expression was analyzed by quantitative and end-point RT-PCR. Intracellular flow cytometry, immunofluorescence and immunohistochemistry were carried out to verify α-defensin 1-3 protein expression in mononuclear cells from peripheral blood and skin infiltrates. α-Defensin 1-3 concentration was evaluated in plasma and blister fluid samples by ELISA.We herein describe DEFA1A3 gene expression in peripheral blood mononuclear cells (PBMCs) from patients with drug-induced cutaneous diseases. Gene expression analysis unveiled transcription in CD4 and CD8 peripheral blood T cells. Protein expression was confirmed by intracellular flow cytometry in mononuclear cells from the patients, including monocytes, NK cells, and T cells from peripheral blood and blister fluid. Further analysis of protein content by flow cytometry revealed higher protein levels in CD56(+) CD3(+) lymphocytes from patients with SJS/TEN when compared to MPE and healthy donors. Immunohistological analysis was used to confirm expression in dermal infiltrates. α-Defensin levels were estimated by ELISA to be 3- to 175-fold higher in blister fluid when compared to simultaneously drawn plasma samples.Upregulation of innate immune molecules such as α-defensins 1-3 in T cells from patients with SJS/TEN may be involved in the etiopathology of these life-threatening diseases induced by medications.
Biological clock dysfunction exacerbates contact hypersensitivity in mice
Authors: E. Takita, S. Yokota, Y. Tahara, A. Hirao, N. Aoki, Y. Nakamura, A. Nakao, S. Shibata
Journal: British Journal of Dermatology (2012)

Immediate-type skin allergic reactions, such as passive cutaneous anaphylactic reaction, are associated with circadian rhythm, but the role of circadian mechanisms on delayed-type skin allergic reactions, such as contact hypersensitivity (CHS), remains uncertain. In mice, CHS, a T-cell-mediated immune response, is a classic model of human allergic contact dermatitis. We investigated whether biological clock dysfunction affects CHS pathogenesis in CLOCK mutant mice compared with wild-type (WT) mice. Mice were treated with 2,4,6-trinitro-1-chlorobenzene (TNCB) on the abdominal skin on day 0 (sensitization) and then treated with TNCB on the ears on day 5 (challenge).We found that biological clock dysfunction resulted in severe inflammation. Ear swelling, serum immunoglobulin E level and mast cell number were significantly increased in CLOCK mutant mice compared with WT mice. These results provide evidence that CLOCK mutation promotes the T-helper type 2 immune response and exacerbates CHS. Corticosterone has a protective effect on CHS. The serum corticosterone level lost rhythmicity and showed a decreased daily level in CLOCK mutant mice compared with WT mice, supporting the exacerbating effect of CLOCK mutation on CHS. Adrenalectomy markedly worsened TNCB-induced CHS in WT mice but not in CLOCK mutant mice. In addition, dramatic dexamethasone-induced protection of CHS was observed in CLOCK mutant mice compared with WT mice. The present results suggest that circadian rhythm might be an important factor in the regulation of CHS via corticosterone rhythmicity and/or level.
Abstract P022: Uncontrolled Hypertension and Lifestyle Factors among US Adults with Disabilities
Authors: Alissa Stevens, Elizabeth Courtney-Long, Dianna Carroll, Cathleen Gillespie, Brian Armour
Journal: Circulation (2015)

Introduction: While hypertension is a key treatable risk factor for cardiovascular disease, it is not controlled in an estimated 36 million US adults. Previous research has shown that nearly half of adults with disabilities have hypertension and that adults with disabilities are more likely to have hypertension than those without disabilities. However, no study has documented the prevalence of uncontrolled hypertension among the disability population. Our objectives were 1) to determine the prevalence of uncontrolled hypertension among adults with a disability, and 2) estimate the prevalence of awareness, treatment with blood pressure (BP)-lowering medication, and lifestyle factors among adults with disabilities who have uncontrolled hypertension. Methods: Using nationally-representative data from the 2001-2010 National Health and Nutrition Examination Survey for 10,805 participants aged ≥20 years with a disability (self-reported limitation in cognition, hearing, vision, or mobility), we examined the prevalence of hypertension (measured systolic BP ≥140, diastolic BP ≥90 or self-reported use of BP-lowering medication) and uncontrolled hypertension (systolic BP ≥140 or diastolic BP ≥90). Among those with uncontrolled hypertension, we estimated the prevalence of awareness (ever told by a doctor that had hypertension), treatment (self-reported use of BP-lowering medication), and lifestyle factors (measured body mass index and dietary sodium intake and self-reported aerobic physical activity and cigarette smoking). Results: Nearly 38% of US adults have a disability. Overall 46.0% (nearly 37 million) of US adults with disabilities have hypertension. Of those, nearly 20 million (52.4%) had uncontrolled hypertension. Over half of those with uncontrolled hypertension were aware and treated (52.9%), 13.6% were aware but untreated, and 33.4% were unaware. Among those with uncontrolled hypertension 40.5% were obese, 52.1% were physically inactive (had no bouts of aerobic physical activity per week that lasted ≥10 minutes), 18.2% were current smokers, and 62.0% had an average sodium intake of ≥2,300 mg per day. Conclusion: Over half of the 37 million adults with disabilities who have hypertension do not have it controlled; and of those, one third are unaware they have hypertension. This study highlights the need to regularly measure and monitor blood pressure among adults with disabilities. It also identifies adults with disabilities as an important population to include in public health efforts that support and encourage healthy behaviors that might improve BP control and lower risk for cardiovascular disease.
Identification of radiosensitivity predictive genes in gastric cancer cell line using gene expression profiling
Authors: Han Sang Kim, Sun Young Rha, Sun Jeong Kim, Chan Hee Park, Jae Kyung Roh, Hyun Cheol Chung, Joong Bae Ahn, Hei-Cheul Jeung, Sang Joon Shin, Yong Bae Kim
Journal: Clinical Cancer Research (2010)

Abstract Background: In the post-genome era, the development of a radiation sensitivity predictive assay is significant as it could lead to better patient and dose selection in radiotherapy. Methods: To identify radiosensitivity predictive genes in gastric cancer cells, 12 gastric and 4 colorectal cancer cell lines were used. Oligonucleotide microarray was performed using a human oligo chip containing 22,740 oligonucleotide probes of 70 bases with a reference design before radiation. Clonogenic survival assays with 2Gy of radiation were performed and survival fraction at 2 Gy (SF2) was measured. Differentially expressed genes were identified through Significant analysis of microarrays (SAM) between radiosensitive and radioresistant cell lines and functional study through Ingenuity pathway analysis was conducted. Results: According to SF2, SNU-638 (0.127±0.038), MKN-1 (0.143±0.032), and HCT-116 (0.313±0.034) were classified as radiosensitive cells, whereas YCC-2 (0.609±0.046), YCC-16 (0.62±0.091), HT29 (0.641±0.070), and YCC-7 (0.667±0.192) were determined as radioresistant cells. Eighty-two up-regulated and one hundred and forty-two down-regulated genes were identified in radiosensitive cell lines compared to radioresistant cell lines. Cell death (43 genes), cellular growth and proliferation (36 genes), cell-to-cell signaling (16 genes), cell cycle (13 genes), and DNA repair (6 genes) were related with identified gene set. Genetic network via TGFB1 and TP53 showed important role in radiosensitivity. Conclusions: Our results suggest that identified genes are candidates for radiosensitivity biomarker discovery and in vitro clonogenic assay with expression analysis may assist to predict responsiveness to radiotherapy.
From Bird to Boy
Authors: Rebecca Voelker
Journal: JAMA (1998)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 1790: Small molecule inhibitors of QSOX1 suppress tumor cell growth and invasion
Authors: Paul D. Hanavan, Douglas O. Faigel, Chen-Ting Ma, Eduard Sergienko, Nathalie Meurice, Joachim L. Petit, Yvette W. Ruiz, Benjamin A. Katchman, Douglas F. Lake
Journal: Cancer Research (2014)

Abstract Introduction: QSOX1 is a flavin-dependent sulfhydryl oxidase and the only known human enzyme with both disulfide generating and transferring activities. Using tissue microarrays, we previously demonstrated that QSOX1 is over-expressed in multiple tumor types including breast, pancreas, prostate and myeloma. Loss of QSOX1 leads to a significant decrease in tumor cell proliferation and invasion. The over-expression of QSOX1 in tumor cells compared to non-malignant cells combined with its role in tumor cell proliferation and invasion make it an attractive target for potential anti-neoplastic agents. Methods: Using a high throughput screening assay to detect sulfhydryl oxidase activity, we screened a library of compounds for inhibition of the QSOX1 enzyme. We used ROS-GloTM, a luciferase-based assay that detects H2O2, to determine if any compounds in the library inhibited QSOX1-mediated disulfide bond formation using reduced RNAse A as a substrate. QSOX1 generates H2O2 as a by-product of disulfide bond formation. To determine if compounds that showed activity in the enzymatic assay were also active against tumor cells, we treated pancreatic, breast, and myeloma cells with the compounds in a dose-dependent fashion, using non-malignant cells as controls. We assayed growth/viability by MTT and CellTiter-Glo assays. Invasive activity of tumor cells was assessed using matrigel-coated inserts in a modified Boyden chamber assay. Results: High throughput library screening identified 4 compounds that specifically inhibited QSOX1 activity (designated A-D) compared to a glucose oxidase counter-screen. Growth/viability assays showed that compound D suppressed the growth of tumor cells with IC50s between 300nM-750nM, depending on tumor cell line. In non-malignant cells the IC50s for compound D were more than 10-fold higher than in tumor cells. Compounds A and D also inhibited invasion of breast and pancreatic tumor cells through Matrigel. Conclusion: This is the first report of chemical compounds that inhibit QSOX1 enzymatic activity. The discovery of QSOX1 inhibitors with cellular efficacy establishes the chemical tractability of the target. Our data demonstrate that these First-In-Class compounds have potential as anti-neoplastic therapeutics via inhibition of QSOX1. Citation Format: Paul D. Hanavan, Douglas O. Faigel, Chen-Ting Ma, Eduard Sergienko, Nathalie Meurice, Joachim L. Petit, Yvette W. Ruiz, Benjamin A. Katchman, Douglas F. Lake. Small molecule inhibitors of QSOX1 suppress tumor cell growth and invasion. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1790. doi:10.1158/1538-7445.AM2014-1790