tbuckley/cabot-search huggingface dataset:
971. New Generation Conditionally Replicative Adenovirus for Hormone-Refractory Prostate Cancer
Authors: Tatyana Gavrikova, Julia Davydova, Long P. Le, Hidetaka A. Ono, Pedro J. Ramirez, Victor Krasnykh, David T. Curiel, Masato Yamamoto
Journal: Molecular Therapy (2004)

Top of pageAbstract
Although anti-androgen therapy is initially effective for advanced prostate cancer, all patients eventually progress to hormone-refractory (HR) prostate cancer (PC) due to biological selection. In this stage, a median survival is only 6-12 months. Therefore, new therapeutic approaches for hormone-refractory prostate carcinoma (HRPC) are needed. Conditionally replicative adenoviruses (CRAds) are attractive anti-cancer agents. These agents are designed to replicate specifically in tumor cells followed by spread of the viral progeny to neighboring cancer cells. We used the cyclooxygenase-2 (COX-2) promoter for transcriptional targeting of tumor, which is active in many cancers but minimally active in normal tissues, including the liver. The COX-2 promoter (-1432/+59: COX-2L) was tested with luciferase (Luc) expression vectors in PC-3, Du-145, and LnCap prostate cancer cell lines. Very strong COX-2 promoter activity comparable to the CMV promoter was observed in PC-3 and Du-145 HRPC cell lines. We next examined infectivity enhancement in the PC cells by using fiber modification of Ad5 vectors, including incorporation of an RGD4C motif into the HI loop (RGD) and replacement of the Ad5 knob region with the Ad3 knob (Ad5/3 chimera),. Three CMV promoter driven Luciferase expression vectors with the wild type fiber (Ad5Luc1), RGD insertion (AdRGDLuc1), and Ad5/3 chimera fiber (Ad5/3Luc1) were used. The RGD-modified vector did not show enhanced infectivity in either of these PC cell lines, while the Ad5/3 chimera demonstrated significantly higher levels of infection in PC-3 and Du-145 HRPC cells. To analyze the effect of fiber modification on the cytocidal effect of CRAds we constructed COX-2 CRAds with wild type, RGD-modified, and Ad5/3 chimeric fibers, respectively. All COX-2 CRAds showed replication and subsequent oncolytic killing in both PC-3 and Du-145 cell lines while the COX-2 negative LnCap cell line was not affected. Ad5/Ad3 chimera CRAds demonstrated the strongest cytocidal effect among COX-2 CRAds in PC3 and Du-145 cell lines. Analysis of in vivo anti-tumor efficacy was performed using Du-145 prostate carcinoma subcutaneous xenografts. COX-2 CRAd agents with unmodified, RGD-modified, or Ad5/3 chimera fibers as well as wild type Ad5 and a non-replicative E1 deleted vector were used for intratumoral administration (5×109 vp/tumor). Our data indicate that the anti-tumor effect of COX-2 CRAd with Ad5/3 chimera fiber was better than unmodified or RGD-modified CRAds. The present results demonstrate that selective replication of CRAd under the control of the COX-2 promoter and infectivity enhancement with Ad5/3 chimeric fiber may be combined to generate promising viral therapeutic agents for the treatment of hormone refractory prostate carcinomas.
Effects of radium-223 dichloride (Ra-223) with docetaxel (D) versus D on prostate-specific antigen (PSA) and bone alkaline phosphatase (bALP) in patients (pts) with castration-resistant prostate cancer (CRPC) and bone metastases (mets): A phase 1/2a clinical trial.
Authors: Michael J. Morris, Celestia S. Higano, Howard I. Scher, Christopher Sweeney, Emmanuel S. Antonarakis, Daniel H. Shevrin, Charles J. Ryan, Yohann Loriot, Karim Fizazi, Neeta Pandit-Taskar, Jose E. Garcia-Vargas, Kari Lyseng, Marianne Bloma, Anne-Kirsti Aksnes, Jorge A. Carrasquillo
Journal: Journal of Clinical Oncology (2015)

202 Background: Ra-223 is an approved α-emitter prolonging survival in CRPC with symptomatic bone mets. We conducted a phase 1/2a study examining the safety and antitumor effects of Ra-223 + D vs D alone, and previously presented data showing that Ra-223 + D is safe and well tolerated (ESMO 2014). Here we report the effect of Ra-223 + D vs D on bALP and PSA dynamics. Methods: D-eligible pts with progressing CRPC and ≥ 2 bone mets were randomized 2:1 to Ra-223 (50 kBq/kg q 6 wk × 5) + D (60 mg/m 2 q 3 wk × 10) vs D (75 mg/m 2 q 3 wk with step-down option to 60 mg/m 2 ). bALP and PSA were recorded q 3 wk during first 6-wk cycle, then q 6 wk and q 3 wk, respectively, and analyzed at a central laboratory. Changes in both markers are described by the % of pts who achieved ≥ 30%, &gt; 50%, and &gt; 80% declines between baseline and the safety follow-up visit (3 wk post last D injection) as their best response; pts with elevated baseline bALP (≥ 21 µg/L) levels were included for the bALP analysis. bALP to below the upper limit of normal (ULN) was also recorded, regardless of % decline. Results: 46 pts (33 Ra-223 + D vs 13 D alone) were enrolled. As of October 2014, 21 (Ra-223 + D) vs 5 (D) pts had received all planned study treatment. Median (range) baseline PSA was 99 µg/L (3-1000) for Ra-223 + D pts and 43 µg/L (4-1042) for D pts. Maximal changes in PSA and bALP levels between baseline and safety follow-up are shown in Table. No pt had a bALP increase. Conclusions: Ra-223 + D appears to favorably impact posttreatment PSA and bALP declines. Ra-223 + D appears particularly effective at normalizing bALP levels vs D alone. The clinical benefits of such changes in serum markers will require validation in larger prospective studies. Clinical trial information: NCT01106352. [Table: see text]
Lymph-Node Bacilliform Bodies Resembling Those of Whipple's Disease in a Patient without Intestinal Involvement
Authors: CHARLES M. MANSBACH
Journal: Annals of Internal Medicine (1978)

A 38-year-old man developed symptoms of arthralgias and arthritis, lymphadenopathy, and weight loss. An axillary lymph-node biopsy was done in the diagnostic study; a periodic acid Schiff stain, done for evidence of fungal infection, showed periodic acid Schiff reagent-positive macrophages. Electron microscopy showed the typical morphologic features of the bacilliform bodies associated with Whipple's disease to be present in the macrophages of the lymph node. The patient had no intestinal symptoms. The absorption of a variety of substrates was found to be normal. Nine intestinal biopsies showed no organisms similar to those found in his lymph node. On tetracycline therapy, he symptomatically improved. The findings raise the question of the route of infection in Whipple's disease and point up the usefulness of periodic acid Schiff staining of lymph-node biopsies.
Fractionated total body irradiation and high-dose VP 16-213 followed by allogeneic bone marrow transplantation in advanced leukemias
Authors: N Schmitz, W Gassmann, M Rister, W Johannson, M Suttorp, F Brix, JJ Holthuis, W Heit, B Hertenstein, J Schaub
Journal: Blood (1988)
Surgical Care of Pediatric Patients in the Humanitarian Setting
Authors: Maeve O’Neill Trudeau, Emmanuel Baron, Patrick Hérard, Amy S. Labar, Xavier Lassalle, Carrie Lee Teicher, David H. Rothstein
Journal: JAMA Surgery (2015)

Little is known about the scope of practice and outcomes in pediatric surgery performed by humanitarian organizations in resource-poor settings and conflict zones. This study provides the largest report to date detailing such data for a major nongovernmental organization providing humanitarian surgical relief support in these settings.To characterize pediatric surgical care provision by a major nongovernmental organization in specialized humanitarian settings and conflict zones.A retrospective cohort study was conducted from August 15, 2014, to March 9, 2015, of 59,928 surgical interventions carried out from January 1, 2012, to December 31, 2013, by the Médecins Sans Frontières Operational Centre Paris (MSF-OCP) program in 20 locations, including South Sudan, Yemen, Syria, Gaza, Pakistan, Nigeria, Central African Republic, Democratic Republic of Congo, and the Philippines. Surgical interventions were primarily for general surgical, traumatic, and obstetric emergencies and were categorized by mechanism, type of intervention, American Society of Anesthesia risk classification, and urgency of intervention.Operative indications, type of intervention, and operative case mortality.Among all age groups, 59,928 surgical interventions were performed in dedicated trauma, obstetric, and reconstructive centers for 2 years. Nearly one-third of interventions (18,040 [30.1%]) involved preteen patients (aged <13 years) and 4571 (7.6%) involved teenaged patients (aged 13-17 years). The proportion of violence-related injuries in the preteen group was significantly lower than in the teenage group (4.8% vs 17.5%; P < .001). Burns (50.1%), other accidental injuries (16.4%), and infections (23.4%) composed the bulk of indications in the preteen group. Interventions in the teenage group were principally caused by trauma-related injuries (burns, 22.9%; traffic accidents, 10.1%; gunshot wounds, 8.0%). Crude perioperative case mortality rates were 0.07% in the preteen group, 0.15% in the teenage group, and 0.22% in the adult group (>17 years) (P = .001). One-third of the cases (33.4%) were deemed urgent, while most of the remaining cases (57.7%) were deemed semielective (surgical intervention to be performed within 48 hours).When examining surgical interventions in a population of pediatric patients cared for in the specialized setting of humanitarian aid and conflict zones, burns, other accidental injuries, and infection composed the bulk of indications in the preteen group; interventions in the teenage group were principally caused by trauma-related injuries. Crude perioperative case mortality rates in the preteen group were significantly lower than in the adult group. Further work is needed to examine long-term outcomes of pediatric operations in these settings and to guide context-specific surgical program development.
Abstract 11515: Characterization of Heart Mitochondrial Proteome Elucidates Significant Changes in Cardiac Energy Metabolism and Antioxidant Prdx3 in Human Dilated Cardiomyopathy
Authors: Miguel Rivera, Esther Rosello, Maria Eugenia Barderas, Estefania Tarazon, Micaela Molina, Ana Ortega, Luis Rodriguez Padial, Manuel Portoles
Journal: Circulation (2014)

Introduction: Dilated cardiomyopathy (DCM) is a public health problem with no available curative treatment, and mitochondrial dysfunction plays a critical role in its development. Methods: LV tissue samples were obtained from 17 patients with DCM. Eight non-diseased donor hearts were used as CNT samples. The protein expressions of purified heart mitochondria from 8 DCM patients and 8 CNT were compared using two-dimensional differential gel electrophoresis (2DDIGE). Results: We encountered 16 significantly upregulated and 5 downregulated spots in DCM hearts. A selection of representative proteins of each mitochondrial function, localization was validated using different techniques that compared its levels among DCM patients (n = 17) and CNT (n = 8). Therefore, using western blot techniques, we determined the levels of some relevant proteins involved in metabolism, including pyruvate dehydrogenase E1 component subunit alpha, somatic form (ODPA); ETFD; DLDH; delta-1-pyrroline-5-carboxylate dehydrogenase (AL4...
Abstract 937: Antigenically-Defined Subsets of Bone Marrow Mesenchymal Stem Cells Exhibit Differential Cardiomyogenic and Angiogenic Potential
Authors: Ewa K Zuba-Surma, Ahmed Abdel-Latif, Robert J Vincent, Smita Ranjan, Sumit Tiwari, Mamdouh Khayat, Roberto Bolli, Buddhadeb Dawn
Journal: Circulation (2006)

Bone marrow mesenchymal stem cells (BMMSCs) are typically isolated as adherent cells; the importance of antigen expression on cardiomyogenic potential of (BMMSCs) remains unclear. Although BMMSCs d...
Distinctions Between CD8+ and CD4+ T-Cell Regenerative Pathways Result in Prolonged T-Cell Subset Imbalance After Intensive Chemotherapy
Authors: Crystal L. Mackall, Thomas A. Fleisher, Margaret R. Brown, Mary P. Andrich, Clara C. Chen, Irwin M. Feuerstein, Ian T. Magrath, Leonard H. Wexler, Dimiter S. Dimitrov, Ronald E. Gress
Journal: Blood (1997)
ERRORS IN CARDIOVASCULAR ROENTGEN-RAY INTERPRETATION
Authors: Hugo Roesler
Journal: Annals of Internal Medicine (1936)

Article1 September 1936ERRORS IN CARDIOVASCULAR ROENTGEN-RAY INTERPRETATIONHUGO ROESLER, M.D.HUGO ROESLER, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-10-3-299 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptErrors in cardiovascular roentgen interpretation are due mostly to the following reasons: Insufficient knowledge of the theoretical and applied physical principles of the roentgen-rays; insufficient knowledge of anatomy, physiology and pathology of the cardiovascular system; and deficiency in the technical outfit and procedures.Facts which pertain to the roentgenological study may not be registered and therefore the first part of the report, which should contain all findings, is inadequate. Or facts are registered but interpreted erroneously and therefore the second part of the report, which should contain the conclusions, is inadequate.The limitations of the roentgenological method permit one to... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Philadelphia, Pennsylvania*Read at the Detroit meeting of the American College of Physicians, March 3, 1936.From the departments of Roentgenology and Medicine, Temple University Hospital, Philadelphia, Pennsylvania. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 September 1936Volume 10, Issue 3Page: 299-305KeywordsCardiovascular physiologyHospital medicine ePublished: 1 December 2008 Issue Published: 1 September 1936 PDF downloadLoading ...
Flow‐Cytometric Analysis of Human Basophil Degranulation
Authors: Takemasa Nakagawa, Beda M. Stadler, Alain L. de Weck
Journal: Allergy (1981)

Quantification of human basophils and their degranulation were performed using a flow‐cytometric system. It was shown that the number of basophils among total leukocytes, before and after the effect of degranulating agents, can be obtained rapidly and reproducibly, following alcian blue, staining. Dose‐response curves of degranulation by anti‐IgE and anti‐IgG 4 were studied, and it was shown that these antibodies can induce basophil degranulation in a Ca ++ dependent manner. It was also shown that degranulation is completed within 10 min. Values obtained by flow‐cytometry and microscopic evaluation, as well as comparison made between percent basophil degranulation and percent histamine release, agreed well with each other in our system. Flow‐cytometric analysis of human basophil degranulation provides a new reliable method to assess in vitro the morphological basis of immediate hyper‐sensitivity.
Pregnant women in poor countries face domestic violence
Authors: Paul Ress
Journal: BMJ (2005)

<h3>BACKGROUND AND PURPOSE:</h3> A lower radiation dose can have a detrimental effect on the quality of head CT images. The aim of this study performed in a pediatric population was to test whether an image-processing algorithm (Correlative Image Enhancement) based on the correlation among intensities of neighboring pixels can improve gray-white differentiation in head CTs. <h3>MATERIALS AND METHODS:</h3> Sixty baseline head CT images with normal findings obtained from scans of 30 children were processed using Correlative Image Enhancement to produce corresponding enhanced images. Gray-white differentiation in baseline and enhanced images was assessed quantitatively by calculating the contrast-to-noise ratio and conspicuity in equivalent ROIs in gray and white matter. Two masked readers rated the images for visibility of gray-white differentiation on a 5-point Likert scale. Differences in both quantitative and qualitative measures of gray-white differentiation between baseline and enhanced images were tested for statistical significance. <i>P</i> values &lt; .05 were considered significant. <h3>RESULTS:</h3> Image processing resulted in improvement in the contrast-to-noise ratio (from 1.86 ± 0.94 to 2.26 ± 1.00, <i>P</i> = .02) as well as conspicuity (from 37.28 ± 11.56 to 46.4 ± 11.5, <i>P</i> &lt; .001). This was accompanied by improved subjective visibility of gray-white differentiation as reported by both readers (<i>P</i> &lt; .01). <h3>CONCLUSIONS:</h3> Image processing using Correlative Image Enhancement had a beneficial effect on quantitative measures of gray-white differentiation. This translated into improved perception of gray-white differentiation by readers. Further studies are needed to assess the effect of such image processing on the detection of disease processes using head CTs.
THE RESPONSIBILITIES OF THE INTERNIST
Authors: D P BARR
Journal: Annals of Internal Medicine (1947)

Article1 August 1947THE RESPONSIBILITIES OF THE INTERNISTDAVID P. BARR, F.A.C.P.DAVID P. BARR, F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-27-2-195 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptFELLOWS OF THE AMERICAN COLLEGE OF PHYSICIANS:By custom I am permitted as your President to address you on this occasion of the Annual Convocation, and in the few moments at my disposal should like to outline a few of the accomplishments of the College and also to indicate some of the responsibilities which we as Internists may assume in our development. Before doing so, however, I should be neglectful of opportunity if I failed to express to you my profound appreciation of the honor of being allowed during the past year to serve in this high office. Like my... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: New York, N. Y.*Presidential Address delivered at the Convocation of the American College of Physicians, Chicago, April 30, 1947. Nextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byCo-morbidity does not reflect complexity in internal medicine patientsInternal medicine: yesterday, today, and tomorrow 1 August 1947Volume 27, Issue 2Page: 195-201 Issue Published: 1 August 1947 Copyright & PermissionsCopyright, 1947, by The American College of PhysiciansPDF downloadLoading ...
A NEW CONCEPT IN THE TREATMENT OF PENICILLIN REACTIONS: USE OF PENICILLINASE
Authors: R M BECKER
Journal: Annals of Internal Medicine (1958)

Article1 June 1958A NEW CONCEPT IN THE TREATMENT OF PENICILLIN REACTIONS: USE OF PENICILLINASER. M. BECKER, M.D., F.A.C.P.R. M. BECKER, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-48-6-1228 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptA previous report1 described the in vivo action of penicillinase on circulating penicillin in guinea pigs and human subjects without reactions. The following is a report of the clinical effectiveness of penicillinase in humans with penicillin reactions.Penicillinase2is an enzyme specific for its substrate penicillin. Its inactivating action against penicillin has been utilized for years in the clinical laboratory in bacteriologic media to inhibit penicillin in body fluids to be cultured. Penicillinase can be obtained from several strains of bacteria, including staphylococci andEscherichia coli. Commercially it is produced in greatest abundance from cultures ofBacillus cereus, strain NRRL-B...Bibliography1. Becker RM: Effect of penicillinase on circulating penicillin, New England J. Med. 254: 952-953 (May 17) 1956. CrossrefMedlineGoogle Scholar2. Levy GB: Automatic measurement of optical rotation, Anal. Chem. 23: 1089-1092, 1951. CrossrefGoogle Scholar3. Schepartz SA: Nature of binding of penicillin by bacterial cells, thesis, State University of Wisconsin, 1955. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Madison, Wisconsin*Received for publication May 17, 1957.†The penicillinase used in these studies was generously supplied by Schenley Laboratories, Inc., New York, N. Y., through the courtesy of Dr. B. Marr Lanman, Director of Clinical Research, and Dr. Bruno Puetzer, Director of Research.Requests for reprints should be addressed to R. M. Becker, M.D., 3414 Monroe Street, Madison 5, Wisconsin. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byThe past, present, and future of enzyme-based therapiesDie AntibioticaRHEUMATISM AND ARTHRITIS: REVIEW OF AMERICAN AND ENGLISH LITERATURE OF RECENT YEARS (THIRTEENTH RHEUMATISM REVIEW)*CHARLEY J. SMYTH, M.D., F.A.C.P., ROGER L. BLACK, M.D., FELIX E. DEMARTINI, M.D., IVAN F. DUFF, M.D., F.A.C.P., EPHRAIM P. ENGLEMAN, M.D., F.A.C.P., DONALD C. GRAHAM, M.D., F.R.C.P.(C), MAX M. MONTGOMERY, M.D., F.A.C.P., HOWARD F. POLLEY, M.D., F.A.C.P., EDWARD F. ROSENBERG, M.D., F.A.C.P., CARLOS F. SACASA, M.D., F.A.C.P., OTTO STEINBROCKER, M.D., HANS WAINE, M.D., GEORGE M. WILSON JR., M.D.Development of sensitivity to penicillinase following its use in penicillin reaction 1 June 1958Volume 48, Issue 6Page: 1228-1242KeywordsAdrenocorticotropic hormoneBacillusBacteriaBody fluidsClinical laboratoriesEnzymesEscherichia coliGuinea pigsPenicillin ePublished: 1 December 2008 Issue Published: 1 June 1958 PDF downloadLoading ...
Abstract 3799: Urokinase kringle-derived peptide UP-7 potently inhibits angiogenesis and tumor growth
Authors: Hyun-Kyung Kim, Purevjargal Naidansuren, Seung Woo Lee, Young Ae Joe
Journal: Cancer Research (2014)

Abstract The recombinant kringle domain of urokinase-type plasminogen activator (UK1) has been shown to inhibit angiogenesis in vitro and in vivo and suppress brain tumor in vivo. UK1 consists of 83 amino acids and has 2 short helices, rudimentary ß-sheets and one extended antiparallel ß-sheets regions. In order to identify functional core sequence of the UK1 for angiogenesis inhibition, we examined the anti-angiogenic and anti-tumor activities for the seven UK1-derived peptides. Among the tested peptides, UP-7 potently inhibited the proliferation and migration of endothelial cells in vitro and suppressed vessel formation in Matrigel plugs in vivo. However, such anti-angiogenic activities were not exerted by the scrambled peptide UP7-SC7 or UP7-derived shorter peptides. UP-7 also suppressed VEGF-induced phosphorylation of FAK and ERK1/2 in endothelial cells. In a NCI-H460 lung cancer xenografted animal model, UP-7 effectively inhibited tumor growth. In addition, treatment of mice bearing MDA-MB231 tumor with UP-7 peptide resulted in a dose-dependent inhibition of tumor growth. Taken together, these results suggest that UK1- derived novel peptide UP-7 can be used for treatment of cancers. (NRF-2012R1A1A2007175). Citation Format: Hyun-Kyung Kim, Purevjargal Naidansuren, Seung Woo Lee, Young Ae Joe. Urokinase kringle-derived peptide UP-7 potently inhibits angiogenesis and tumor growth. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3799. doi:10.1158/1538-7445.AM2014-3799