tbuckley/cabot-search huggingface dataset:
Expression profile of cornified envelope structural proteins and keratinocyte differentiation-regulating proteins during skin barrier repair
Authors: H.D. de Koning, E.H. van den Bogaard, J.G.M. Bergboer, M. Kamsteeg, I.M.J.J. van Vlijmen-Willems, K. Hitomi, J. Henry, M. Simon, N. Takashita, A. Ishida-Yamamoto, J. Schalkwijk, P.L.J.M. Zeeuwen
Journal: British Journal of Dermatology (2012)

Recent studies have emphasized the importance of heritable and acquired skin barrier abnormalities in common inflammatory diseases such as psoriasis and atopic dermatitis (AD). To date, no comprehensive studies on the effect of experimental barrier disruption on cornified envelope protein expression have been performed.To analyse the effect of experimental skin barrier disruption on the expression of cornified envelope structural proteins and keratinocyte differentiation-regulating proteins.We examined mRNA (day 1, 3 and 7) and protein (day 1, 2, 4 and 9) expression levels of structural proteins and regulatory molecules after sodium dodecyl sulphate (SDS) application on normal skin, and tape stripping of uninvolved epidermis of patients with psoriasis and AD and healthy controls.Upon tape stripping, several structural molecules were significantly downregulated (at the mRNA level as well as the protein level), including LCE5A, LCE2B, FLG, FLG2 and LOR, whereas others were upregulated: IVL, SPRR1, SPRR2, HRNR and most notably LCE3A. The epidermal crosslinking enzymes TGM1, TGM3 and TGM5 were all upregulated, whereas proteases involved in the desquamation process (CTSV, KLK5 and KLK7) were downregulated or unaffected. Most results were similar in SDS-instigated irritant contact dermatitis. There was no significant difference in response between normal epidermis and nonlesional skin of patients with psoriasis and AD.Skin barrier disruption induces a temporary barrier repair response composed of increased expression of several cornification-related proteins, and decreased expression of some structural and desquamation-related proteins.
Tumor-Bound Immunoglobulins: An In Vivo Phenomenon of Masked Specificity 2
Authors: G. Dorval, I. P. Witz, E. Klein, H. Wigzell
Journal: JNCI Journal of the National Cancer Institute (1976)

We examined the specificity of the Ig “coating” on murine tumor cells grown in vivo. Cells were treated in vitro for release of cell-bound Ig from a.scites tumors. Such uncoated cells showed an increased expression of tumor-associated antigens and a parallel decrease in intensity of the Ig coat, but displayed no changes in the expressions of other normal membrane antigens. This was shown by a complement-dependent cytotoxicity assay and radioimmunoassay. These changes were attenuated if the tumor originated from animals that had been irradiated before tumor inoculation. No alterations were found with corresponding cells propagated in vitro and submitted to the same treatments. Our findings and others suggest tumor-specific antibodies among the Ig coats detected on tumor cells grown in vivo.
Cardiac resynchronization therapy in heart failure patients with less severe left ventricular dysfunction
Authors: Ofek Y. Hai, Robert J. Mentz, Faiez Zannad, Maurizio Gasparini, Gaetano M. De Ferrari, Jean‐Claude Daubert, Johannes Holzmeister, Carolyn S.P. Lam, Thierry Pochet, Alphons Vincent, Cecilia Linde
Journal: European Journal of Heart Failure (2014)
A clinical isolate of <i>Leishmania donovani</i> with ITS1 sequence polymorphism as a cause of para-kala-azar dermal leishmaniasis in an Ethiopian human immunodeficiency virus-positive patient on highly active antiretroviral therapy
Authors: T. Gelanew, W. Amogne, T. Abebe, K. Kuhls, A. Hailu, G. Schönian
Journal: British Journal of Dermatology (2010)

The diagnosis of para‐kala‐azar dermal leishmaniasis (PKDL/VL), either as an immune reconstitution inflammatory syndrome (IRIS)‐associated syndrome or as a complication of visceral leishmaniasis (VL) during human immunodeficiency virus (HIV) co‐infection in patients with or without highly active antiretroviral therapy, represents a challenge for prompt treatment. The aim of this study was to identify the causative Leishmania species and to clarify whether the post‐kala‐azar dermal leishmaniasis (PKDL)‐like lesions appeared as a result of IRIS or not. A 31‐year‐old Ethiopian male patient, with stage IV HIV/acquired immune deficiency syndrome (AIDS), was clinically diagnosed with PKDL/VL. He had developed a generalized maculopapular rash on his face after initiation of highly active antiretroviral therapy. The Leishmania isolate obtained from the skin lesions was analysed by polymerase chain reaction–restriction fragment length polymorphism (PCR‐RFLP) and sequencing of the ribosomal DNA internal transcribed spacer 1 (ITS1) and partial coding sequences of the heat shock protein 70 gene (hsp70). Restriction analysis of the ITS1 PCR product gave a unique RFLP pattern not seen before for any Leishmania isolate. Sequencing of both the ITS1 and hsp70 PCR products identified the causative species as Leishmania donovani, and further revealed the existence of five different sequence variants of the ITS1 among the 10 clones sequenced. The results indicate that PKDL/VL resulted from an infection by L. donovani. The sequence variants of ITS1 might be due to the presence of multiple strains/clones or the existence of intragenomic variations in the multicopy ITS1, or a combination of both.
Identification of driver genes in hepatocellular carcinoma by exome sequencing
Authors: Sean P. Cleary, William R. Jeck, Xiaobei Zhao, Kui Chen, Sara R. Selitsky, Gleb L. Savich, Ting-Xu Tan, Michael C. Wu, Gad Getz, Michael S. Lawrence, Joel S. Parker, Jinyu Li, Scott Powers, Hyeja Kim, Sandra Fischer, Maha Guindi, Anand Ghanekar, Derek Y. Chiang
Journal: Hepatology (2013)

Genetic alterations in specific driver genes lead to disruption of cellular pathways and are critical events in the instigation and progression of hepatocellular carcinoma (HCC). As a prerequisite for individualized cancer treatment, we sought to characterize the landscape of recurrent somatic mutations in HCC. We performed whole-exome sequencing on 87 HCCs and matched normal adjacent tissues to an average coverage of 59×. The overall mutation rate was roughly two mutations per Mb, with a median of 45 nonsynonymous mutations that altered the amino acid sequence (range, 2-381). We found recurrent mutations in several genes with high transcript levels: TP53 (18%); CTNNB1 (10%); KEAP1 (8%); C16orf62 (8%); MLL4 (7%); and RAC2 (5%). Significantly affected gene families include the nucleotide-binding domain and leucine-rich repeat-containing family, calcium channel subunits, and histone methyltransferases. In particular, the MLL family of methyltransferases for histone H3 lysine 4 were mutated in 20% of tumors. Conclusion: The NFE2L2 - KEAP1 and MLL pathways are recurrently mutated in multiple cohorts of HCC. (Hepatology 2013;58:1693–1702)
684. Expanded Parvoviral Replicons as Novel Nonviral Vectors for Cancer Gene Therapy
Authors: Boris R. A. Blechacz, Jean Rommelaere, Christiane Dinsart, Brandenburger Annick, Russell J. Stephen
Journal: Molecular Therapy (2004)

Gene therapy is an appealing approach to the treatment of human malignancies but significant barriers exist. The use of viral gene therapy vectors is limited due to immunogenicity and toxicity. Nonviral gene transfer systems achieve only low transduction efficiencies. Autonomous parvoviruses are known for their oncotropism and oncosuppressive potential. In addition, recombinant parvoviruses provide high level transgene expression. We therefore developed and optimized parvoviral replicon vectors based on the autonomous parvovirus Minute Virus of Mice (MVMp) and MVMp/EBV (Epstein Barr virus) hybrid vectors. The use of parvoviral replicon vectors will take advantage of the tumor cell-specific cytotoxicity of parvoviruses. Gene delivery of these replicons as SPLP-liposomes will be nontoxic and non-immunogenic allowing repetitive administration. Transgene size, normally limited by inefficient encapsidation of recombinant parvoviruses exceeding a total size of 106% of the wildtype, is not limited in these parvoviral replicon vectors. The parvoviral genome is excised, replicated and expressed in tumor cells resulting in high plasmid copy numbers and high-level transgene expression. Comparison to other highly potent expression vectors, replicative and non-replicative, showed the desirable characteristics of parvoviral replicon vectors. We expect high level cytotoxicity on tumor cells with major bystander effects by using these MVMp-based replicon vectors for expression of the human sodium iodide symporter gene (hNIS) and the Gibbon ape leukaemia virus fusogenic membrane glycoprotein gene (Galv-FMG). Both of these therapeutic genes have proved their strong antitumoral potential in different tumor-models in the past. By packaging these therapeutic MVMp-replicons in SPLP-liposomes we expect a highly efficient vector for cytoreductive cancer gene therapy. Results: MVMp and MVMp/EBV-hybrid replicon vectors containing the marker genes CEA, luciferase and GFP and the therapeutic transgenes Galv-FMG and hNIS have been cloned. Excision and replication of parvoviral DNA has been shown as early as 24 h after transfection. High-level transgene expression in different cell lines was achieved. Excision, replication and transgene expression has been shown up to an increase of the parvoviral genome size of 355% of the wildtype. Comparison to CMV-promoter driven replicative and non-replicative expression vectors showed higher and longer transgene expression levels by the parvoviral replicon vectors. Transgene expression by MVMp and MVMp/EBV-hybrid vectors increased over time in dependence of the susceptibility of the cell line towards MVMp and EBV. Parvoviral replicons containing Galv-FMG were shown to have a very strong cytotoxic potential resulting in an almost complete destruction of susceptible cell layers. Transduction of cells with hNIS expressing parvoviral replicon vectors resulted in high level 125I-uptake.
Late-breaking abstract: Significant association between protein C promoter region polymorphism and susceptibility to pulmonary thromboembolism in a Chinese Han population
Authors: Jinming Liu, Yafang Miao
Journal: European Respiratory Journal (2011)

Pulmonary thromboembolism (PTE) is a common clinical problem that is associated with substantial morbidity and mortality. Because protein C (PC) play an essential role in regulation of thrombin activity, we investigated the role of PC polymorphism in patients with pulmonary thromboembolism. One hundred and ten cases of PTE and one hundred and ninety healthy control in Chinese Han population were genotyped for three polymorphisms (-1654C/T, -1641A/G and -1476A/T) of PC promoter. Using Binary logistic regression analysis, genetic risk factor, which was homozygous carriers of genotype TT (the SNP site -1654C/T) of the PC gene, and conventional risk factors (“operation and trauma” and “operation and trauma”) were independent predictors of the development of PTE. PC gene SNPs (-1654C/T, -1641A/G and -1476A/T) in control region are probably associated with the susceptibility to PTE in Chinese Han population. The homozygous carriers of genotype TT of -1654C/T was significantly associated with the outcome of PTE, which together with “operation and trauma” and “operation and trauma” were have a great risk of developing serious PTE.
Abstract 16643: Right Ventricular Systolic Dysfunction Assessed by Cardiac Magnetic Resonance is a Strong Predictor of Cardiovascular Death After Coronary Bypass Surgery
Authors: Anne-Catherine M Pouleur, Michel F Rousseau, Sylvie A Ahn, Fabien Demeure, Mihaela Amzulescu, Christophe de Meester, David Vancraeynest, Agnès Pasquet, Jean-Louis Vanoverschelde, Bernhard L Gerber
Journal: Circulation (2014)

BACKGROUND:  Although increased left ventricular (LV) volumes and reduced ejection fraction (EF) are associated with adverse outcomes in heart failure (HF), there are limited data regarding the prognostic value of right ventricular (RV) systolic dysfunction (RVSD) in ischemic cardiomyopathy. The purpose of this study was to evaluate the impact of RV function assessment by cardiac magnetic resonance (CMR) before surgical revascularization on survival in patients with coronary artery disease (CAD) and low EF.   METHODS:  We prospectively evaluated survival of 107 consecutive patients (94 males, age 66 ± 10 years) with CAD and LV dysfunction (EF: 25 ± 7%) undergoing CMR prior complete surgical revascularization.   RESULTS:  Mean RV function parameters were respectively: RVEDVi 84±28mL/m2, RVESVi 46±25mL/m2 and RVEF 45±16%. RVSD, defined by RV EF ≤35%, was present in 32 (30%) pts. Perioperative mortality at 30 days (n=6 CV death) was significantly higher in patients with RVSD (13% vs. 3%, p=0.043). During the median follow-up of 4.2 years, 42 pts died (worsening HF n=10, sudden death n=12, other CV death n=8, non CV death n=11), 1 pt underwent heart transplantation. The primary end point of cardiovascular death or cardiac transplantation was reached by 14 of 32 pts with RVSD and 17 of 75 pts without RVSD (44% versus 23%, p=0.028). In univariate analysis, age (1.05, 95% CI 1.008-1.09, p= 0.017), eGFR (0.98, 95% CI 0.97-0.99, p=0.017), LVEF (0.94, 95% CI 0.90-0.99, p=0.031) and RVSD (2.51, 95% CI 1.21-5.12, p=0.011) were predictors of the primary end-point. In a stepwise multivariable analysis adjusting for age, eGFR, and LVEF, RVSD remained a significant independent predictor of the primary end-point (HR 3.57; 95% CI, 1.69-7.52;  p =0.001).   CONCLUSIONS:  RVSD is a strong independent predictor of cardiovascular mortality in patients with ischemic cardiomyopathy undergoing coronary bypass surgery. Evaluation of RV function should thus be performed in all HF patients prior surgery.
Abstract 60: Genotranscriptomic meta-analysis of centromere protein A (CENPA): An essential role in human cancer progression
Authors: Xia Sun, Sifeng Qu
Journal: Cancer Research (2015)

Abstract Background: As the cancer hallmark, genetic instability caused by abnormal cell division, is characterized with the gain or loss of entire chromosomes. The specific histone-H3 variant, centromere protein-A (CENPA), plays essential role in regulating kinetochore assembly, centromere activity and chromosome stability during cell division. However, the biological function of CENPA in human cancer remains largely uncharacterized. This study aims to determine the prognostic value of CENPA expression in cancer patients and the potential therapeutic target in cancer treatment via the genotranscriptomic meta-analysis. Methods: In order to well address the issue, a comprehensive and unbiased genotranscriptomic meta-analysis of CENPA in human cancers was performed with the clinical patient information databases, i.e. COSMIC, Oncomine and cBioPortal. Results: Based on the analysis, it strongly indicated the widespread cancer promoting role of CENPA. There are no gene fusion, very low point mutation (0.15%) and very rare copy number variation (CNV) gain (0.09%) at the CENPA locus in 15214 patient samples across 33 cancer types. However, transcriptomic analyses indicated high correlation of increased CENPA mRNA levels in various solid cancer tissues compared with normal tissues. Furthermore, the poor clinical outcome among 51 clinical cohorts with different cancer types shows significantly correlation with CENPA up-regulation, especially in breast cancer. More importantly, the CENPA up-regulation is also significantly correlated with the metastasis progression and pathology-assessment in breast cancer patients. The high rank of metastatic related genes correlated with CENPA up-regulation is also confirmed in TCGA breast cancer cohorts. Conclusions: The current study highly indicates the essential cancer promoting role of CENPA in human cancer and its value as prognostic marker in clinical use. It also provides rationale for further investigation of CENPA as potential therapeutic target in breast cancer treatment. Citation Format: Xia Sun, Sifeng Qu. Genotranscriptomic meta-analysis of centromere protein A (CENPA): An essential role in human cancer progression. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 60. doi:10.1158/1538-7445.AM2015-60
A CASE OF FATAL BILE PERITONITIS FOLLOWING LIVER NEEDLE BIOPSY
Authors: A I RUBENSTONE, S S MINTZ, D R MERANZE
Journal: Annals of Internal Medicine (1952)

Case Reports1 January 1952A CASE OF FATAL BILE PERITONITIS FOLLOWING LIVER NEEDLE BIOPSYALBERT I. RUBENSTONE, M.D., SOLOMON S. MINTZ, M.D., DAVID R. MERANZE, M.D.ALBERT I. RUBENSTONE, M.D.Search for more papers by this author, SOLOMON S. MINTZ, M.D.Search for more papers by this author, DAVID R. MERANZE, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-36-1-166 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptIt is the purpose of this paper to present a case of fulminating bile peritonitis and death following liver needle biopsy and to point out the hazard of this procedure in cases of obstructive jaundice.No case of death due to bile peritonitis following liver needle biopsy has as yet been reported. Several authors list this danger as a theoretical possibility (Roholm, Krarup and Iversen,1Hoffbauer2). The former believe that in two instances of liver needle biopsy they aspirated pure bile from either a large dilated bile duct or from the gall-bladder. Since the first use of liver needle biopsy...Bibliography1. RoholmKrarupIversen HNP: Aspiration-biopsie der Leber mit einer Uebersicht über die Ergebnisse bei 297 Biopsien, Ergebn. d. inn. Med. u. Kinderh. 61: 635, 1942. Google Scholar2. Hoffbauer FW: Needle biopsy of the liver, J. A. M. A. 134: 666, 1947. CrossrefMedlineGoogle Scholar3. Lucatello L: Sulla puncture de figato a scopo diagnostico, Lavori del Congressi di medicina interna, Rome, 1895. Google Scholar4. Klofer W: Die Leberpunktion als brauchbare und wertvolle klinische Untersuchungsmethode, Klin. med. 138: 774, 1940. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Philadelphia, Pennsylvania*Received for publication February 22, 1950.From the Laboratories of The Mount Sinai Hospital of Philadelphia. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byCase Series: Biliary Leak After Transjugular Liver BiopsyInjuries of the gall-bladderBile leakage as a complication of liver biopsy in liver transplantsBile peritonitisNeedle Biopsy of the LiverNeedle Biopsy of the Liver 1 January 1952Volume 36, Issue 1Page: 166-168KeywordsBileBile ductsBiopsyLiverPeritonitis ePublished: 1 December 2008 Issue Published: 1 January 1952 PDF downloadLoading ...
Therapy of Anastomotic Leaks by Means of Covered Self-Expanding Metallic Stents after Esophagogastrectomy
Authors: J M Doniec, B Schniewind, V Kahlke, B Kremer, H Grimm
Journal: Endoscopy (2003)

Background and Study Aims: The mortality rate for surgical revision of gastroesophageal anastomotic leakage after resection for cancer approximates 60 %. The efficacy of endoscopically placed covered metallic stents for treatment of gastroesophageal leakage was evaluated. Patients and Methods: Between June 1996 and June 2002 we treated 21 patients with proven gastroesophageal leakage; 18 had anastomotic leakage and three patients had perforation for different reasons. The extent of the leaks ranged from one-quarter of the intestinal circumference to its complete dehiscence. The average time from surgery to detection of leakage was 6.1 days (range 3 - 15 days). Mortality, healing rate, length of hospital stay, and complications were assessed. Results: The insertion of stents was performed endoscopically under radiological guidance without any complication in all patients. In 9.5 % (2/21) of patients complete sealing of the leak was not achieved. The mortality associated with anastomotic leakage was 23.8 % (5/21). In 80.1 % (17/21) patients complete healing of the leakage was achieved. The average hospital stay was 67 days (range 14 - 158 days). Of 23 stents, 13 (56.5 %) were removed, and three patients developed stenosis after removal. Conclusion: The treatment of gastroesophageal leakage with covered stents appears to reduce mortality and the complication rate associated with major leakage. Therefore this technique seems to be a reasonable alternative in the treatment of clinically relevant anastomotic leakage.
Characterization of Peri-Infarct Zone Heterogeneity by Contrast-Enhanced Multidetector Computed Tomography
Authors: Karl H. Schuleri, Marco Centola, Richard T. George, Luciano C. Amado, Kristine S. Evers, Kakuya Kitagawa, Andrea L. Vavere, Robert Evers, Joshua M. Hare, Christopher Cox, Elliot R. McVeigh, João A.C. Lima, Albert C. Lardo
Journal: Journal of the American College of Cardiology (2009)
A common polymorphism in the promoter of UCP2 is associated with decreased risk of obesity in middle-aged humans
Authors: Harald Esterbauer, Clemens Schneitler, Hannes Oberkofler, Christoph Ebenbichler, Bernhard Paulweber, Friedrich Sandhofer, Gunther Ladurner, Emanuel Hell, A. Donny Strosberg, Josef R. Patsch, Franz Krempler, Wolfgang Patsch
Journal: Nature Genetics (2001)
Military Medicine in the Balkans Now
Authors: P Gunby
Journal: JAMA (1999)

KOSOVO—The Bosnian crud has some potential new targets. “The crud” is what US soldiers with the Stabilization Force in Bosnia and Herzegovina call the upper respiratory tract infections that are diagnosed whenever new troops arrive. Its latest targets may be more than 3200 10th Mountain Division troops who completed their deployment to the former Yugoslavian province in October from Fort Drum, New York. They are relieving the Fort Hood, Texas– based 1st Cavalry Division. Army physicians say that the crud’s viral infections require 2 or 3 days of bed rest, fluids, and medication for the symptoms, which include a cough that may persist for nearly a month. After that, most soldiers seem to develop some degree of immunity, which physicians hope again will be the case with the Bosnian winter looming. However, next March, a new group of peacekeepers from the Texas Army National Guard’s 49th Armored Division will arrive and the crud may reappear. In any case, as the North Atlantic TreatyOrganization(NATO)peacekeeping effort in Bosnia nears the close of its fourth year—already four times longer than originally announced—the number of US troops involved and, therefore, exposed to upper respiratory tract or other infections has declined. Sometime this winter, the total of American troopsmaydecline toa lowofabout4000 from a high of about 20 000 in 1995.