tbuckley/cabot-search huggingface dataset:
Abstract 862: Ser517:-phosphorylation Of Sirt1 Is Required For Its Pi3k/akt-mediated Nuclear Translocation And Cardiomyocyte Protection Against Oxidant Stress In Failing Hearts.
Authors: Masaya Tanno, Tetsuji Miura, Takayuki Miki, Toshiyuki Yano, Yoshiyuki Horio, Kazuaki Shimamoto
Journal: Circulation (2007)

[Purpose] We recently found that SIRT1, a protein deacetylase, shuttles between the nucleus and cytoplasm. In this study, we examined the role of nuclear SIRT1 in cardiomyocyte protection against oxidant stress and involvement of PI3K/Akt in the nuclear translocation. [Methods and Results] First, the critical intracellular location of SIRT1 for its anti-apoptotic function was examined. C2C12 cells were transfected with wild-type SIRT1 (WT) or SIRT1 with site-directed mutations in the nuclear localizing signal (mtNLS) and exposed to antimycin A (AA), an oxidative stressor. AA-induced apoptosis was suppressed in WT-transfected cells expressing SIRT1 in the nuclei compared with that in mtNLS-transfected cells expressing SIRT1 in the cytoplasm (TUNEL-positive cells = 4.4±0.7% vs. 34.6±8.0%). AA-induced apoptosis and also angiotensin II-(angII)-induced apoptosis in neonatal rat cardiomyocytes (NRCM) were suppressed by resveratrol, a SIRT1 activator. This protective effect of resveratrol was attenuated by transfection of SIRT1-siRNA but not by transfection of control siRNA. Next, we assessed the role of PI3K/Akt in nuclear translocation of SIRT1. SIRT1 in NRCM was localized in both the nucleus and cytoplasm under baseline conditions, and IGF-1 induced its nuclear translocation. This effect of IGF-1 was suppressed by LY294002 (LY), a PI3K inhibitor. Deletion mutagenesis study showed that LY-induced nuclear exclusion was observed for SIRT1[223–540] but not for SIRT1[223– 489]. Replacement of serine517 with alanine (S517A) increased cytoplasmic SIRT1, and S517A showed attenuated nuclear translocation in response to IGF-1, indicating that serine517 is the target site of PI3K/Akt. Finally, to confirm heart failure-associated SIRT1 translocation in vivo, myocardial infarction was induced in WKY rats. The number of ventricular cardiomyocytes with nuclear SIRT1 at 4 weeks after infarction was significantly larger than that in sham-operated hearts (10.2±2.9% vs. 0.7±0.2%). [Conclusion] The results suggest that phosphorylation of SIRT1 at Ser517 by PI3K/Akt is involved in nuclear translocation of SIRT1, which contributes to cardiomyocyte protection from oxidant stress-mediated injury in failing hearts.
Can an Amulet Cure Leukemia?
Authors: F Rosner
Journal: JAMA (1999)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
FRI0569-HPR The Effectiveness of Education and Aerobic Exercise in Higher Functioning Patients with Fibromyalgia: an Evaluation of A New Service
Authors: C. Cullinane, J.G. McVeigh, C. Sheehy, B. Irundaj
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background</h3> Fibromyalgia syndrome (FMS) is characterised by chronic widespread pain, fatigue and reduced physical function. Exercise has positive effects on physical function, fatigue and well-being in FMS<sup>1</sup>. However, patients find it difficult to engage in exercise and often have low self-efficacy for exercise. <h3>Objectives</h3> The aim of this study was to evaluate a new early access service consisting of a 6-week education and aerobic exercise intervention in patients with FMS, who were classified as "high functioning". <h3>Methods</h3> Participants (n=32) were referred from WRH rheumatology department and attended a 6-week exercise and education intervention delivered by physiotherapist and occupational therapist. Participants completed the Fibromyalgia Impact Questionnaire (FIQ), Hospital Anxiety and Depression Score (HAD) Six-Minute-Walk Test (6MWT) and Exercise Self-Efficacy Scale (ESES). Follow up was carried out post and 3 months post programme. <h3>Results</h3> Participants who completed the intervention had a significant improvement in mean (SD) pain from baseline 7.01 (1.8) to the end of the programme 5.6 (2.9), p=0.03. Mean (SD) 6MWT improved from 386m (96) to 416m (57) at the end of the programme (p=0.05), this was maintained at 3 month follow-up. There was no significant change in total FIQ score or the HAD scale, however, participants9 exercise self-efficacy significantly improved from 58.2 (18.6) to 68 (11.6), p=0.01. <h3>Conclusions</h3> Improvements were recorded post programme in exercise capacity, exercise self-efficacy and VAS pain. Improvement was maintained at 3 months follow up. Anticipated service developments are use of FIQ to subgroup<sup>2</sup>, longer term follow up, vocational rehabilitation and pedometer use to evaluate exercise adherence.<sup>3</sup> <h3>References</h3> Hauser, W., Klose, P., Langhorst, J., Moradi, B., Steinbach, M., Schiltenwolf, M. &amp; Busch, A. (2010) Efficacy of different types of aerobic exercise in fibromyalgia syndrome: a systematic review and meta-analysis of randomized controlled trials. Arthritis Research &amp; Therapy, 12(3), R79. De Souza, (2009) Fibromyalgia Subgroups: Profiling distinct subgroups using the Fibromyalgia Impact Questionnaire. A Preliminary Study. Rheumatology Int.29:509-515 Tudor-Locke C., Craig C., Aoyagi Y., (2011). How many steps/day are enough? For older adults and special populations. A literature Review. International Journal of Behavioural Nutrition and Physical Activity 8:80 <h3>Disclosure of Interest</h3> None declared <h3>DOI</h3> 10.1136/annrheumdis-2014-eular.2931
Abstract 3458: Role of the EphB1 gene in mediating migration, proliferation, and radiosensitization of medulloblastoma cells
Authors: Nimrah A. Baig*, Olga Timofeeva*, Anatoly Dritschilo, Elena Pasquale, Marcel Kool, Brian Rood, Olga Rodriguez, Christopher Albanese^, Sana Karam^
Journal: Cancer Research (2014)

Abstract Eph/ephrin signaling in a variety of cancers has been reported to promote aspects of tumorigenesis, including proliferation, migration, and angiogenesis. Medulloblastoma is an aggressive primitive neuroectodermal tumor originating in the cerebellum. Recently performed integrative genomic approach to a large cohort of medulloblastomas has identified four subtypes (A-D) based on clinical presentation, transcription profiles, genetic abnormalities, and clinical outcome. We show high levels of EphB1 expression in tumors derived from children with group D medulloblastomas, which are known to exhibit a poorer response to current therapies. The purpose of this study is to examine the effect knockdown of EphB1 has on cellular migration, proliferation, and radiosensitization of medulloblastoma tumors. Using a human-derived medulloblastoma cell line (DAOY) and small interfering RNA (siRNA) targeting EphB1, we compared cell migration in an electrical impedance-based transwell Boyden chamber assay (xCELLigence RTCA DP system). We show that siRNA knockdown of EphB1 results in significantly decreased migration. Assessment of proliferation by MTT assay indicates that knockdown of EphB1 affects cell cycle progression in DAOY. Results from cell cycle and sub-G1 analysis by flow cytometry demonstrate that knockdown of EphB1 results in G1 arrest. Our previous systems analysis investigation of global gene expression regulated by an intact ATM gene product also identified EphB1 as a downstream signaling molecule correlating with enhanced cellular radiation resistance. Our data strongly support EphB1 as an unequivocal target for radiation sensitization in human fibroblasts as well as in vitro medulloblastoma cell lines. siRNA knockdown of EphB1 expression in human medulloblastoma cell lines known to express high levels of EphB1 results in sensitization to ionizing radiation by clonogenic survival assays. Preliminary data from an in vivo xenograft medulloblastoma animal model suggest an enhanced response to radiation in tumors lacking the EphB1 gene. Our data indicate that EphB1 is a potential molecular target for medulloblastoma cancer treatment and radiosensitization. The mechanisms and downstream signaling cascades underlying EphB1's action on cell migration, proliferation, and cellular radiosensitization are currently under investigation. Citation Format: Nimrah A. Baig*, Olga Timofeeva*, Anatoly Dritschilo, Elena Pasquale, Marcel Kool, Brian Rood, Olga Rodriguez, Christopher Albanese^, Sana Karam^. Role of the EphB1 gene in mediating migration, proliferation, and radiosensitization of medulloblastoma cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3458. doi:10.1158/1538-7445.AM2014-3458
Abstract 15742: Predicting Outcomes After Percutaneous Mitral Balloon Valvotomy: The Impact of Left Ventricular Strain Imaging
Authors: Sergio Barros-Gomes, Mackram F Eleid, Jordi S Dahl, Cristina Pislaru, Rick A Nishimura, Patricia A Pellikka, Sorin V Pislaru
Journal: Circulation (2014)

Introduction: Presence of subclinical left ventricle (LV) dysfunction may influence outcomes after percutaneous mitral balloon valvotomy (PMBV), but cannot be detected by conventional echocardiography. Hypothesis: Preprocedure LV global longitudinal strain (GLS) by speckle tracking TTE detects subclinical LV dysfunction and predicts long-term outcome after successful PMVB. Methods: Consecutive patients undergoing PMBV between 2003-2011 who had comprehensive baseline TTE were included. Patients with suboptimal immediate results (repeat PMBV or mitral surgery within first 6 months) and those with suboptimal TTE strain were excluded. LV strain was analyzed with commercial software (Syngo VVI), and GLS averaged from 18 segments. The primary end point was all-cause mortality. Secondary end point was the composite of death and mitral valve reintervention. Results: Of 110 patients screened, 29 (26%) had unsuitable strain imaging, and 8 (7%) had poor immediate results, leaving 73 in the final cohort. Median follow-up was 3.7 years (interquartile range 1.3-7.2). There were 10 deaths (14%) and 25 late mitral valve interventions (34%). Univariate predictors of death were GLS, age, EF, stroke volume, pulmonary artery pressure, mitral valve area and coronary artery disease. In a multivariate Cox model, GLS was the strongest predictor of death after adjustment for age [HR=1.40 (1.09-1.84), P=0.008]. For the composite endpoint, GLS, mitral valve gradient, and invasively mean left atrial pressure were univariate predictors of death or reintervention. At multivariate analysis, GLS was the sole predictor of events [HR=1.17 (1.01-1.34), P=0.03]. Event-free survival is shown in figure. Intra and inter observer intra-class correlation for strain analysis was 0.87 (0.66-0.96) and 0.84 (0.53-0.95), respectively. Conclusions: GLS is a powerful predictor of long-term outcome after PMVB and appears to have an incremental prognostic value over traditional parameters.
Less Surgery, Improved Survival From Stage IV Colorectal Cancer?
Authors: Rachel Wong, Peter Gibbs
Journal: JAMA Surgery (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal
THE RELATION OF THE CRUSH SYNDROME TO THAT OF BURNS AND OTHER TYPES OF TRAUMATIC WOUNDS OF HUMAN TISSUES
Authors: Walter Estell Lee
Journal: Annals of Internal Medicine (1943)

Article1 June 1943THE RELATION OF THE CRUSH SYNDROME TO THAT OF BURNS AND OTHER TYPES OF TRAUMATIC WOUNDS OF HUMAN TISSUESWALTER ESTELL LEE, M.D.WALTER ESTELL LEE, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-18-6-991 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe purpose of this paper is to call attention to evidence which is gradually accumulating that such diverse traumatic injuries as (1) blast, (2) burns, (3) crush, and (4) trauma of the soft tissues all probably have at least two etiological factors in common: ( 1 ) The loss or escape of plasma from the circulating blood stream, and (2) a toxemia resulting from degenerative changes and products liberated from dying or dead tissue cells, plasma and bacteria.The subject chosen, the relation of thecrush syndrometo that ofburnsand othertypes of traumatic woundsof human tissues,... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Philadelphia, Pennsylvania*Read before the meeting of the Postgraduate Nights of the American College of Physicians held at the Naval Hospital, Philadelphia, October 22, 1942. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 June 1943Volume 18, Issue 6Page: 991-996KeywordsBacteriaBlood cellsBlood plasmaBurnsCellsRiversSoft tissuesTraumatic injury ePublished: 1 December 2008 Issue Published: 1 June 1943 PDF downloadLoading ...
Abstract P078: Effects Of Individual Dietary Factors On Metabolic Risks Within The Context Of Overall Dietary Patterns: A Systematic Review And Meta-analysis
Authors: Gitanjali M Singh, Marcia C de Oliveira Otto, Dariush Mozaffarian
Journal: Circulation (2014)

Background: Effects of dietary factors on cardiometabolic risk are often studied in isolation. Yet, multiple dietary components are often consumed together, which may alter effects of individual dietary factors. Objective: To quantify effects of 7 diet components (fruits, vegetables, nuts/seeds, whole grains, fish, dietary fiber) when consumed within the context of overall dietary patterns on major metabolic risks. Methods: Using MOOSE and PRISMA guidelines, we systematically searched multiple databases to identify randomized controlled feeding studies evaluating effects of diet patterns on cardiometabolic risk factors. We focused on trials evaluating major diet patterns that included fruits, vegetables, nuts/seeds, whole grains, fish, and dietary fiber as major components and tested effects on BP, lipids, BMI or waist circumference, or glucose-insulin homeostasis (fasting glucose, HbA1c, oral glucose tolerance test, postprandial glucose, fasting insulin). Studies were excluded if &lt;4 weeks, utilized diet advice rather than feeding, involved other major interventions, relied on self-reported outcomes, or included only patients with cachectic or end-stage disease. Multivariate inverse-variance weighted meta-regression estimated the pooled effects of individual diet components, when consumed together, on cardiometabolic risks. Results: Meta-regression analyses of 6 intervention arms in 3 large dietary pattern trials demonstrated significant effects of several individual components (Table) Pooled results including additional dietary pattern trials and results for adiposity and glucose/insulin homeostasis are to be shown. Conclusions: This systematic assessment quantifies the impact of individual dietary factors on cardiometabolic risks within the context of overall diet patterns, more accurately reflecting complementary effects of multiple components and informing magnitudes of benefits for preventive policies and interventions.
Mosquito Eaters Curb Dengue
Authors: Joan Stephenson
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 1586: Ezetimibe Reduces Hepatic Steatosis in Diet-Induced Obese Mice
Authors: Joyce J Hwa, Lu Yang, Melanie Henry, Glen Tetzloff, Lizbeth Hoos, John Cook, Li-Ji Zhu, Margaret van Heek, Nicholas Murgolo, Thomas Hosted, Diane Levitan, Marsha Smith, Scott Altmann, Harry R Davis
Journal: Circulation (2006)

Ezetimibe is a novel cholesterol absorption inhibitor which reduces plasma LDL-cholesterol by selectively binding to the intestinal cholesterol transporter, Niemann-Pick C1-Like 1 (NPC1L1). Mice deficient in NPC1L1 are protected from high fat diet-induced fatty liver as well as hypercholesterolemia. The object of this study was to determine whether ezetimibe treatment can reduce hepatic steatosis in diet-induced obese (DIO) mice and to explore the underlying mechanism(s). C57BL/6J mice were fed a high fat/cholesterol diet (Research Diets, with 45% Kcal fat and 0.12% w/w cholesterol) for 7 months after weaning. After 4 weeks, the body weight of DIO mice treated with ezetimibe (5.6 mg/kg/day in the high fat/cholesterol diet) was not significantly different from control mice. However, liver wet weight and the liver to body weight ratio were significantly reduced by 18% in the ezetimibe-treated DIO mice. Livers from ezetimibe-treated mice had significantly lower cholesteryl esters (−84%), free cholesterol (C, −47%) and triglyceride (TG, −20%). After 4 weeks of ezetimibe treatment, total plasma cholesterol and triglyceride was significantly reduced by 30% and 15%, respectively. There were significant decreases in VLDL-C and LDL-C, while HDL-C was not changed in the ezetimibe-treated group. Although the VLDL-C was significantly reduced after ezetimibe treatment, the VLDL-TG production rates were comparable between ezetimibe-treated and control DIO mice. Unlike human liver, mouse liver did not express NPC1L1 under chow or high fat/cholesterol feeding. Microarray analysis demonstrated a significant up-regulation of genes responsible for cholesterol biosynthesis and a modest but significant up-regulation of genes in the citrate cycle and β-oxidation pathway. In conclusion, chronic ezetimibe treatment can significantly reduce hepatic steatosis in mice fed high fat/cholesterol diet by decreasing liver cholesteryl esters, free cholesterol and TG levels, in addition to the reduction of plasma cholesterol and TG levels. Ezetimibe is not only an effective treatment for dyslipidemia, but may also be useful for reducing high fat diet-induced hepatic steatosis.
Sequential chemoimmunotherapy with cisplatin, interleukin-2, and interferon alfa-2a for metastatic melanoma.
Authors: D Khayat, C Borel, J M Tourani, A Benhammouda, E Antoine, O Rixe, E Vuillemin, P A Bazex, L Thill, R Franks
Journal: Journal of Clinical Oncology (1993)

PURPOSE To evaluate the activity and the toxicity of the combination of cisplatin (CDDP)/recombinant interleukin-2 (rIL-2) and interferon alfa-2a (IFN alpha) in disseminated malignant melanoma (DMM). PATIENTS AND METHODS Between December 1990 and March 1992, 39 patients with biopsy-proven metastatic malignant melanoma (MM), bidimensionally measurable lesions and an Eastern Cooperative Oncology Group (ECOG) performance status &lt; or = 2 entered this protocol. Seventy-nine percent had received previous chemotherapy including platinum complex (15%) and alpha interferon (44%). They received CDDP (100 mg/m2 on day 0) followed by IL-2 18.10(6) IU/m2/d continuous intravenous (IV) infusion from day 3 to day 6 and from day 17 to day 21. The cycle was repeated on day 28. Subcutaneous IFN alpha 9.10(6) IU three times weekly was administered throughout the treatment period. From day 66 or 94, patients were administered a maintenance cycle with CDDP 100 mg/m2, subcutaneous IL-2 5.10(6) IU/m2/d from day 15 to day 19 and from day 22 to day 26 and IFN alpha 9.10(6) IU three times weekly repeated every 5 weeks (maximum four cycles). RESULTS Among 39 assessable patients, five patients achieved complete responses (CRs). Sixteen patients had partial responses (PRs). The overall objective response rate was 53.8%. The number of metastatic sites was the only response-predictive factor. Toxicity was manageable in a routine patient setting and there was no life-threatening toxicity. CONCLUSION These results seem to indicate a possible synergy between CDDP/rIL-2 and IFN alpha in MM.