tbuckley/cabot-search huggingface dataset:
Lack of association between three vascular endothelial growth factor gene polymorphisms and systemic sclerosis: results from a multicenter EUSTAR study of European Caucasian patients
Authors: Y Allanore, D Borderie, P Airo, S Guiducci, L Czirjak, E L Nasonov, G Riemekasten, P Caramaschi, M Majdan, D Krasowska, E Friedl, H Lemarechal, L P Ananieva, T Nievskaya, O G Ekindjian, M Matucci-Cerinic, A Kahan
Journal: Annals of the Rheumatic Diseases (2006)

Systemic sclerosis (SSc) is characterised by disturbed vessel morphology and an overproduction of vascular endothelial growth factor (VEGF). The VEGF gene located on chromosome 6p21.3 has several polymorphisms.To test the hypothesis that disturbed angiogenesis may be related to the genetic background of the VEGF gene.EUSTAR centres included European Caucasian patients with SSc and matched controls with osteoarthritis. The VEGF gene was genotyped by polymerase chain reaction, followed by restriction enzyme analysis. The 634 C/T and 936 C/G mutations and an 18-base pair insertion/deletion at -2549 of the VEGF promoter region were tested.416 patients with SSc and 249 controls were included in the study population. Of the patients with SSc, 42% had a diffuse cutaneous subtype, 16% had increased pulmonary arterial pressure and 61% had decreased carbon monoxide diffusion capacity. The genotype frequencies in the patients with SSc and in controls were in Hardy-Weinberg equilibrium. The allele and genotype frequencies of the polymorphisms did not differ between patients with SSc and controls. No association was found between these polymorphisms and disease phenotypes.This study shows that there is no association between the three selected functional VEGF polymorphisms and SSc.
Codeine Intoxication in Infancy
Authors: Kelley K. Davis
Journal: New England Journal of Medicine (1961)

CODEINE preparations, widely used for the symptomatic treatment of diseases in infants and children, are safe and effective agents in cases selected and controlled, with administration in the recommended dosage. The opium phenanthrene alkaloids, however, all possess therapeutic disadvantages that may create serious difficulties if the fundamentals of drug dosage and usage are ignored. Especially in infants attention should be paid to the deleterious effects of cumulative action, idiosyncrasy, tolerance and addiction. The former two are particularly pertinent. Drug idiosyncrasy is a common phenomenon in the pediatric age group, and codeine is far from an exception; frequent dosage, large dosage . . .
Dog mastocytoma cells secrete a 92-kD gelatinase activated extracellularly by mast cell chymase.
Authors: K C Fang, W W Raymond, S C Lazarus, G H Caughey
Journal: Journal of Clinical Investigation (1996)

Gelatinolytic metalloproteinases implicated in connective tissue remodeling and tumor invasion are secreted from several types of cells in the form of inactive zymogens. In this report, characterization of gelatinase activity secreted by the BR line of dog mastocytoma cells reveals a phorbol-inducible, approximately 92-kD, Ca2+ - and Zn2+ -dependent proenzyme cleaved over time to smaller, active forms. Incubation of cells with the general serine protease inhibitor, PMSF, prevented proenzyme cleavage and permitted its purification free of activation products. The NH2-terminal 13 amino acids of the purified mastocytoma progelatinase are 50-67% identical to those of human, mouse, and rabbit 92-kD progelatinase (gelatinase B; matrix metalloproteinase-9). Degranulation of mastocytoma cells using ionophore A23187 greatly accelerated proenzyme cleavage, suggesting that a serine protease present in secretory granules hydrolyzed the progelatinase to active fragments. To identify the activating protease, cells were coincubated with ionophore and a panel of selective serine protease inhibitors. Soybean trypsin inhibitor and succinyl-L-Ala-Ala-Pro-Phe-chloromethylketone, which inhibit mast cell chymase, prevented progelatinase activation. Inhibitors of tryptase and dog mast cell protease (dMCP)-3, i.e., aprotinin or bis(5-amidino-2-benzimidazolyl) methane (BABIM), did not. In further experiments using highly purified enzymes, mastocytoma cell chymase activated 92-kD progelatinase in the absence of other enzymes or cofactors; tryptase and dMCP-3, however, had no effect. These data demonstrate that dog mastocytoma cells secrete a metalloproteinase related to progelatinase B that is directly activated outside of the cell by exocytosed chymase, and provide the first demonstration of a cell that activates a matrix metalloproteinase it secretes by cosecreting an activating enzyme. In mastocytomas, this pathway may facilitate tumor invasion of surrounding tissues, and in normal mast cells, it could play a role in tissue remodeling and repair.
What is the Value of Tc-99m Hydroxymethylene Diphosphonate Scintigraphy for the Etiological Diagnosis of Mild Rhabdomyolysis?
Authors: Vincent L.M. Esnault, Magued Nakhla, Catherine Delcroix, Marie-Genevieve Moutel, Olivier Couturier
Journal: Clinical Nuclear Medicine (2007)

We tested whether bone scintigraphy could help in the etiological diagnosis in patients with mild rhabdomyolysis, because different patterns of soft tissue radiotracer uptake have been found on bone scintigraphy in patients presenting with severe rhabdomyolysis, with a localized asymmetric pattern in traumatic lesions and a more diffuse and symmetric pattern in nontraumatic lesions. However, the performance of bone scintigraphy in mild rhabdomyolysis is unknown. The etiological diagnosis of rhabdomyolysis can be difficult, particularly in alcohol abusers who deny a recent alcoholic binge.Bone scintigraphy was performed in 16 patients presenting with acute renal failure secondary to mild rhabdomyolysis (creatinine kinase levels < or =36,000 IU), with obvious causes in 10 cases and with uncertain etiologies in the remaining 6 patients.In 5 cases with evident traumatic rhabdomyolysis, and in 4 patients with uncertain etiologies, bone scintigraphy showed localized asymmetric soft tissue radiopharmaceutical uptake compatible with traumatic lesions. None of the 5 patients with nontraumatic rhabdomyolysis had significant soft tissue radiopharmaceutical uptake. In 1 patient with traumatic rhabdomyolysis, bone scintigraphy performed only 11 days after the initial insult was negative. One patient with an uncertain etiology also had a negative scintigraphy.Bone scintigraphy, when performed early in the course of the disease, may contribute to the etiological diagnosis of rhabdomyolysis if it shows asymmetric localized soft tissue radiopharmaceutical uptake compatible with traumatic lesions. However, bone scintigraphy is often negative in mild nontraumatic lesions.
PWE-079 Dexa Rates And Outcomes In An Ibd Population Cohort. Lessons Learnt
Authors: MN Shuhaibar, C O’Morain
Journal: Gut (2014)

<h3>Introduction</h3> Osteoporosis is one of the under diagnosed and undertreated conditions worldwide. It is estimated that 1 in 3 women and 1 in 5 men in Ireland are diagnosed with it above the age of 50 years and will be likely to become more common with the increased life expectancy. In our study, we aimed to assess the rate of screening with DEXA scans for this condition in a prospective IBD cohort and to evaluate the results. We also studied possible factors that may influence DEXA rates. <h3>Methods</h3> As part of two decades follow up study of a multi-centre Irish IBD prospective cohort from the early 1990’s that was part of European Collaborative Study on IBD (EC-IBD), patients were traced and interviewed. There were 126 IBD patients from the Greater Dublin area who consented and completed the study questionnaire, following Ethical committees’ approvals. In this questionnaire we evaluated DEXA frequency and possible factors that may influence it; including patient diagnosis, age, gender, family history of IBD, courses of steroids, social class, total years of education, attending gastroenterologist or a surgeon, years of loss to follow up (LTFU) and membership to the national IBD patient support group (Irish Society of Crohn’s Colitis ISCC). Results were analysed with logistic regression and forward stepwise analysis using R program 3.0.1 software, taking p value &lt;0.05 to be statistically significant. <h3>Results</h3> Of the total 126 patients only 58 (46.03%) patients had DEXA scans performed. Of those 67.44% had CD and 33.73% had UC. When evaluating DEXA results osteoporosis was diagnosed in 26.67 and 21.43% of CD and UC patients respectively. Osteopenia was diagnosed in 43.33% CD and 42.86% of UC patients. About 30% of remaining scanned patients had normal DEXA. Logistic regression analysis showed that patients with positive family history of IBD and longer years of education were more likely to have had a scan with P = 0.024 and 0.049 respectively. Other possible risk factors didn’t reach statistical significance. Patients who were less compliant and LTFU were less likely to get DEXA with p = 0.045 in CD and p = 0.0336 in UC group. <h3>Conclusion</h3> Osteoporosis is an increasingly recognised condition and recently has been considered as one of the extra intestinal manifestations of IBD. Patient and doctor awareness is paramount to screening and diagnosis. Our study showed that CD patients were more likely to have DEXA scans during their disease course. The rate of osteoporosis was higher in CD patients, despite their fewer numbers when compared to UC group. Over 40% of IBD patients had osteopenia. Early detection and appropriate management will help to reduce fracture risk, improve patient quality of life in a cost effective manner. <h3>Disclosure of Interest</h3> None Declared.
Metabolism of cadmium, zinc and copper in the rat kidney: the role of metallothionein and other binding sites.
Authors: D H Petering, J Loftsgaarden, J Schneider, B Fowler
Journal: Environmental Health Perspectives (1984)

Studies were undertaken to determine the effect of host zinc deficiency upon the distribution of Cd, Zn and Cu between and within male rat kidney cytosol and unfractionated cell pellet. In the first experiment male rates were fed stock diets supplemented with 100 micrograms Cd/mL in the drinking water for 30 days. Then Cd-treated rats and controls were segregated into groups, which received semipurified diets adequate or deficient in zinc for 14 days. After this regimen there were comparable concentrations of total Cd and metallothionein bound Cd in cytosol and the supernatant of sonicated, unfractionated pellet on a microgram Cd/g protein basis. Although less than 5% of cytosolic Cd is not bound to metallothionein (MT), 3-5 times as much non-MT Cd is present in the particulate fraction. The zinc-deficient (Zn-) dietary regime increases the non-MT Cd in the pellet. Quantitations were done of the Cu and Zn distribution in high molecular weight, superoxide dismutase, and metallothionein regions of the profiles of metals from Sephadex G-75 chromatography. In animals exposed to Cd and fed a zinc-normal (Zn+) diet, supernatant and pellet metal contents only change in the MT fraction. Similarly, zinc deficiency affects primarily the complement of metals bound to metallothionein: zinc is markedly decreased and Cu is lowered to a smaller extent. Cadmium is unchanged. Control kidney, unexposed to Cd, normally contains a substantial amount of Zn,CuMT. Two-week zinc deficiency greatly reduces MT-Zn and -Cu content without altering the metal content of other cellular pools.(ABSTRACT TRUNCATED AT 250 WORDS)
Diet Therapy of Diabetes: An Analysis of Failure
Authors: KELLY M. WEST
Journal: Annals of Internal Medicine (1973)

A review of the available evidence shows clearly the rarity with which diabetics understand and follow their diet prescriptions. The reasons for these shortcomings and their persistence are many and complex. They include the tendency of physicians to underestimate the formidability of developing, implementing, and adjusting a diet prescription that is both acceptable and effective over a long period of time. Another problem is the limited conceptual and technical knowledge of most physicians concerning dietary principles, strategies, and tactics as they apply to the various types of diabetes. Recent research confirms the important potentials of diet regulation in mitigating diabetes and its complications. But apparently much of our effort in diet counseling is ineffective and wasteful. It seems desirable, therefore, to review in some detail the reasons for this failure and then to use candid appraisals for developing more effective approaches in the diet therapy of diabetes.
928 DEFICIENCY OF PHOSPHATIDYLINOSITOL 3-KINASE (PI3K) AGGRAVATES INFLAMMATION AND FIBROSIS IN EXPERIMENTAL NON ALCOHOLIC STEATOHEPATITIS BUT PROTECTS AGAINST BILE DUCT LIGATION INDUCED FIBROSIS IN VIVO
Authors: K. Dostert, R. Wiest, J. Schölmerich, C. Hellerbrand, E. Gäbele
Journal: Journal of Hepatology (2010)
Postpericardiotomy Syndrome Following Surgery for Nonrheumatic Heart Disease
Authors: TOMIKO ITO, MARY ALLEN ENGLE, HENRY P. GOLDBERG
Journal: Circulation (1958)

Widespread intracardiac surgery of rheumatic heart disease has brought to medical attention a puzzling postoperative complication, usually designated the "postcommissurotomy syndrome" and often considered to represent reactivation of rheumatic fever. Herein are reported instances of an identical complication following intrapericardial surgery of congenital heart disease. It is suggested that the delayed febrile pleuropericardial reaction represents a traumatic pericarditis and that a preferable name for it is the postpericardiotomy syndrome.
Homicide and Suicide in a Metropolitan County
Authors: Charles S. Hirsch
Journal: JAMA (1973)

Because homicide represents the ultimate deterioration of personal interactions, its frequency in a given population furnishes an objective index of violent reactions to the cumulative stresses in the group. Moreover, problems generated by these tragic losses of life are larger than a simple summation of individual misery. Ultimately, a high rate of homicide disrupts almost every facet of society. Since homicide has such extensive social, legal, and medical ramifications, its study possesses an immediacy matched by comparatively few other aspects of life on the current scene in the United States. Previous studies in Cleveland<sup>1</sup>and elsewhere<sup>2-4</sup>all show that the incidence of homicide is correlated with poverty, with an intimate acquaintance between victim and assailant, and with a preponderance of non-white male victims. A marked increase in the local and national<sup>5</sup>homicide rates during the past decade prompted us to restudy homicide in the Cleveland area, partly
Pessimism Is No Poison
Authors: Lidia Schapira, Phyllis Butow, Richard Brown, Frances Boyle
Journal: Journal of Clinical Oncology (2009)

Dee Jones is 35 years old, married, and the mother of three children, all younger than15 years of age. She had been healthy all of her life before she presented with a 2-week history of abdominal discomfort and anorexia. Evaluation revealed moderate jaundice, an enlarged liver, and abnormal liver function tests. An image of the abdomen showed a pancreatic mass and widespread metastatic disease to the liver. A liver biopsy confirmed a diagnosis of metastatic pancreatic carcinoma. She received first-line chemotherapy for pancreatic cancer and returned 6 weeks later for re-evaluation. She had evidence of disease progression, increasing pain, weight loss, and was bedridden more than 50% of her waking hours. A staff oncologist, accompanied by a first-year oncology fellow, visited with the patient. With the patient acknowledging that she wanted to hear prognostic information, the medical oncologist had a heart-to-heart discussion with the patient. The senior oncologist noted that survival is typically measured in weeks. They discussed treatment options ranging from additional chemotherapy to participation in an early-phase clinical trial. They talked about symptom management and hospice care. They agreed that no further cytotoxic chemotherapy would be used. The conversation was difficult for all: the patient and her husband cried and the oncologist was visibly moved. After leaving the room, the staff oncologist and fellow discussed the consultation. The fellow asked the oncologist why he was so pessimistic when talking with the patient. Why did he say things to make the patient and her husband cry? Shouldn’t he have given the patient more hope? Would it not have been better for the patient to hear this dire news in chunks over the course of several clinic visits? After all, the patient was so young she may just beat the odds.
Absence of viral interference and different susceptibility to interferon between hepatitis B virus and hepatitis C virus in human hepatocyte chimeric mice
Authors: Nobuhiko Hiraga, Michio Imamura, Tsuyoshi Hatakeyama, Shosuke Kitamura, Fukiko Mitsui, Shinji Tanaka, Masataka Tsuge, Shoichi Takahashi, Hiromi Abe, Toshiro Maekawa, Hidenori Ochi, Chise Tateno, Katsutoshi Yoshizato, Takaji Wakita, Kazuaki Chayama
Journal: Journal of Hepatology (2009)
Acute Bronchial Asthma—Response to Treatment as Measured by Symptoms, Signs, and Pulmonary Function Studies.
Authors: Roger W. Kiser
Journal: Annals of Internal Medicine (1972)

Meeting Abstracts1 May 1972Acute Bronchial Asthma—Response to Treatment as Measured by Symptoms, Signs, and Pulmonary Function Studies.Roger W. Kiser, M.D., William J. deGroot, M.D., E. R. McFadden Jr., M.D., F.A.C.P.Roger W. Kiser, M.D.Search for more papers by this author, William J. deGroot, M.D.Search for more papers by this author, E. R. McFadden Jr., M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-76-5-880_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptFourteen asthmatic subjects were studied during acute attacks of bronchospasm to determine when clinical manifestations of the disease would disappear in relation to the changes in pulmonary function. After base-line measurements were taken, each patient was given identical therapy, at hourly intervals, for a minimum of 5 hours, and serial observations were made. We recorded subjective complaints (dyspnea and wheezing) and objective findings (retraction and rhonchi) and correlated them with changes in physiology. Loss of subjective complaints occurred with one to three treatments, and the physiologic data improved as follows: airway resistance (RA), 43% change from baseline; specific conductance (SGa),... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Galveston, Tex. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 May 1972Volume 76, Issue 5Page: 880-880KeywordsDyspneaPulmonary diseasesPulmonary functionSigns and symptoms Issue Published: 1 May 1972 PDF DownloadLoading ...
Bioreducible hyaluronic acid conjugates as siRNA carrier for tumor targeting
Authors: Hong Yeol Yoon, Hyun Ryoung Kim, Gurusamy Saravanakumar, Roun Heo, Su Young Chae, Wooram Um, Kwangmeyung Kim, Ick Chan Kwon, Jun Young Lee, Doo Sung Lee, Jae Chan Park, Jae Hyung Park
Journal: Journal of Controlled Release (2013)
Results of cutting balloon angioplasty for stent restenosis. Japanese multicenter registry
Authors: M. Nakamura, T. Suzuki, T. Matsubara, T. Kobayashi, T. Muramatsu, Y. Asakura, W. Chin, H. Nishikawa, M. Takayama, T. Yamaguchi
Journal: Journal of the American College of Cardiology (1998)
Publication Bias
Authors: Drummond Rennie
Journal: JAMA (1992)

The appearance of an article in the pages ofTHE JOURNALis the result of a complex and lengthy process. Scores of people, acting as investigators, funding agents, members of institutional review boards, authors, reviewers, and editors, make decisions that influence what is published. Each decision may be subject to biases: the conscious and subconscious influences that interfere with impartial judgments. The editors are uncomfortably aware that, for reasons of space and timeliness, the good may often be turned down in favor of the best, but when the final product is published, all concerned like to imagine that their own decisions were free of institutional, hierarchical, political, sexist, racist, and other biases. It has been shown for one journal that these sorts of biases may be largely removed from the editorial process by having reviewers blinded to the names, positions, and institutions of the authors,<sup>1</sup>and an effort is
Decreased levels of heat shock proteins in gut epithelial cells after exposure to plant lectins
Authors: J H Ovelgonne
Journal: Gut (2000)

<h3>BACKGROUND</h3> The enterocytes of the intestinal epithelium are regularly exposed to potentially harmful substances of dietary origin, such as lectins. Expression of heat shock proteins (HSPs) by this epithelium may be part of a protective mechanism developed by intestinal epithelial cells to deal with noxious components in the intestinal lumen. <h3>AIM</h3> To investigate if the lectins PHA, a lectin from kidney beans (Phaseolus vulgaris) and WGA, a lectin from wheat germ (Triticum aestivum) could modify the heat shock response in gut epithelial cells and to establish the extent of this effect. <h3>METHODS</h3> Jejunal tissue sections from PHA and WGA fed rats were screened for expression of HSP70, HSP72, and HSP90 using monoclonal antibodies. Differentiated Caco-2 cells, the in vitro counterpart of villus enterocytes, were exposed to 100 μg/ml of PHA-E<sub>4</sub> or WGA for 48 hours and investigated for changes in DNA and protein synthesis by double labelling with [2-<sup>14</sup>C]thymidine and L-[methyl-<sup>3</sup>H]methionine. The relative concentrations of HSP60, HSP70, HSP72, and HSP90 and binding protein (BiP) in these cells exposed to lectins were analysed by polyacrylamide gel electrophoresis and immunoblotting. To establish if lectin exposed differentiated Caco-2 cells were still capable of producing a heat shock response, these cells received a heat shock (40°C, 41°C, and 42°C) for one hour and were allowed to recover for six hours at 37°C. During heat shock and recovery periods, lectin exposure was continued. <h3>RESULTS</h3> Constitutive levels of HSPs were measured in the intestinal cells of lactalbumin fed (control) rats, as may be expected from the function of this tissue. However, in PHA and WGA fed rats a marked decline in the binding of antibodies against several HSPs to the intestinal epithelium was found. These results were confirmed by in vitro experiments using differentiated Caco-2 cells exposed to PHA-E<sub>4</sub> and WGA. However, after exposure to lectins, these cells were still capable of heat induced heat shock protein synthesis, and total protein synthesis was not impaired indicating specific inhibition of HSP synthesis in non-stressed cells. <h3>CONCLUSIONS</h3> We conclude that PHA and WGA decrease levels of stress proteins in rat gut and enterocyte-like Caco-2 cells, leaving these cells less well protected against the potentially harmful content of the gut lumen.
Ethnic differences in asthma and associated phenotypes: Collaborative Study on the Genetics of Asthma
Authors: Lucille A. Lester, Stephen S. Rich, Malcolm N. Blumenthal, Alkis Togias, Shirley Murphy, Floyd Malveaux, Michael E. Miller, Georgia M. Dunston, Julian Solway, Raoul L. Wolf, Jonathan M. Samet, David G. Marsh, Deborah A. Meyers, Carole Ober, Eugene R. Bleecker
Journal: Journal of Allergy and Clinical Immunology (2001)
Response of ETV6-FLT3–positive myeloid/lymphoid neoplasm with eosinophilia to inhibitors of FMS-like tyrosine kinase 3
Authors: Christoph Walz, Philipp Erben, Michael Ritter, Adrian Bloor, Georgia Metzgeroth, Nick Telford, Claudia Haferlach, Torsten Haferlach, Stefan Gesk, Joannah Score, Wolf-Karsten Hofmann, Andreas Hochhaus, Nicholas C. P. Cross, Andreas Reiter
Journal: Blood (2011)