tbuckley/cabot-search huggingface dataset:
Shaker-related potassium channel, Kv1.4, mRNA regulation in cultured rat heart myocytes and differential expression of Kv1.4 and Kv1.5 genes in myocardial development and hypertrophy.
Authors: H Matsubara, J Suzuki, M Inada
Journal: Journal of Clinical Investigation (1993)

The multiple K+ channels are crucial for repolarization and configuration of the action potential in the neuronal and cardiac cells. In this study, we report the regulatory mechanisms of rapidly inactivating Shaker Kv1.4 channel transcript in the rat heart. Quantitative PCR analysis showed that stimulation with high concentration of KCl, BAY-K 8644, or 12-O-tetradecanoyl phorbol-13-acetate resulted in an immediate and substantial increase (two- to threefold) of Kv1.4 mRNA levels in spontaneously beating myocytes prepared from neonatal rat ventricles. The Kv1.4 mRNA in the ventricle remains at a steady state level after birth and gradually declines with maturation. These results suggest that the Kv1.4 mRNA level is not static and undergoes dynamic modulation by multiple factors that activate intracellular signals. In addition, the expression patterns of Kv1.4 as well as the delayed rectifier Shaker K+ channel Kv1.5 mRNAs were examined in hypertrophied ventricles in which a plateau phase of action potential is remarkably prolonged. The Kv1.5 mRNA level was dramatically repressed while the Kv1.4 mRNA level was remarkably increased. This differential regulation was completely reversed by the normalization of hypertrophy, suggesting that the pathological alterations of K+ channel gene regulation may be involved in the occurrence of ventricular arrhythmias in hypertrophic hearts.
Review: Adding corticosteroids to antibiotics improves pain relief in patients with sore throat
Authors: Magdy W. Attia
Journal: Annals of Internal Medicine (2013)

ACP Journal Club19 March 2013Review: Adding corticosteroids to antibiotics improves pain relief in patients with sore throatMagdy W. Attia, MD, FAAP, FACEPMagdy W. Attia, MD, FAAP, FACEPAlfred I. DuPont Hospital for Children, Wilmington, Delaware, USA, Jefferson Medical College, Philadelphia, Pennsylvania, USA (M.W.A.)Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-158-6-201303190-02011 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Source CitationHayward G, Thompson MJ, Perera R, et al. Corticosteroids as standalone or add-on treatment for sore throat. Cochrane Database Syst Rev. 2012;(10):CD008268. https://pubmed.ncbi.nlm.nih.gov/23076943Clinical Impact RatingsGIM/FP/GP: Emergency Med: Author, Article, and Disclosure InformationAffiliations: Alfred I. DuPont Hospital for Children, Wilmington, Delaware, USA, Jefferson Medical College, Philadelphia, Pennsylvania, USA (M.W.A.)This article was published at Annals.org on 5 March 2013. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 19 March 2013Volume 158, Issue 6Page: JC11KeywordsAdverse eventsAntibioticsChildrenDatabasesPeritonsillar abscessesPharyngitisResearch designSafetySteroidsUpper respiratory tract infections ePublished: 19 March 2013 Issue Published: 19 March 2013 CopyrightCopyright © 2013 by American College of Physicians. All Rights Reserved.PDF DownloadLoading ...
115 CD8 T CELL-MEDIATED IMMUNE RESPONSES AGAINST CROSS-PRESENTING LIVER SINUSOIDAL ENDOTHELIAL CELLS LEADS TO HEPATITIS
Authors: D. Stabenow, K. Gärtner, M. Odenthal, M. John von Freyend, H. Dienes, U. Protzer, P. Knolle
Journal: Journal of Hepatology (2009)
Abstract 3871: Per1 is phosphorylated by ATM/ATR and is involved in the G2/M checkpoint response
Authors: Sigal Gery, Takayuki Tabasyhi, Verena Nowak, Rocio Alvarez, Julia Sohn, Phillip H. Koeffler
Journal: Cancer Research (2010)

Abstract Circadian clocks are found in most organisms and influence a large array of cellular, physiological and behavioral processes. Consequently, disruption of circadian function results in severe dysfunctions and has been associated with various pathologies including cancer. Recent findings revealed intriguing links between the circadian system and another highly conserved biological system, the DNA damage response (DDR). Upon DNA damage, eukaryotic cells activate a complex signaling network to regulate cell cycle checkpoint arrest and/or apoptosis. At the core of the DDR are the ATM, ATR and DNA-PK kinases, which phosphorylate numerous downstream targets on preferred SQ motifs. We previously showed that the key circadian factor, Per1, associates with ATM and sensitize cells to DNA-damage induced apoptosis. In a recent screen, human Per1 Ser1263 was identified as a potential substrate for ATM/ATR. Per1 Ser1263 is part of a SQ motif conserved among mammalian Per1 proteins. To confirm that Per1 is phosphorylated on this site, we mutated murine Per1 Ser1264 (corresponding to human Ser1263) to alanine. 293T and HCT116 cells were transfected with either wild-type (WT) or S1264A mutant (SA) myc-tagged Per1 and treated with DNA-damaging agents. Western blot analysis demonstrated that Per1WT was recognized by phospho-specific SQ antibodies, while Per1SA was not. Furthermore, Per1 phosphorylation was significantly stronger in the treated cells. Interestingly, Per1 remained phosphorylated in ATM deficient cells. In contrast, silencing of ATR by shRNA in 293T cells attenuated Per1 phosphorylation. Immunoprecipitations experiments showed that both overexpressed and endogenously expressed Per1 associate with ATR. To examine the functional significance of Per1 phosphorylation, we transfected SKOV3 cells with either myc-Per1WT or myc-Per1SA, and monitored their cell-cycle progression and apoptotic response after DNA damage. Compared to control SKOV3 treated cells, Per1WT expression resulted in significant increased numbers of G2/M arrested and apoptotic cells. This increase was diminished by Per1S1264A mutation. Our findings implicate Per1 as an important ATM/ATR substrate, and emphasize the significance of proper circadian regulation to cell survival upon exposure to genotoxic stress. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3871.
Autoantibodies against nuclear pore complexes are associated with more active and severe liver disease in primary biliary cirrhosis
Authors: Pietro Invernizzi, Mauro Podda, Pier Maria Battezzati, Andrea Crosignani, Massimo Zuin, Eva Hitchman, Marco Maggioni, Pier Luigi Meroni, Edward Penner, Józefa Wesierska-Gadek
Journal: Journal of Hepatology (2001)
The Impact on Infant Birth Weight and Gestational Age of Cotinine-Validated Smoking Reduction During Pregnancy
Authors: Chang Qing Li
Journal: JAMA (1993)

<h3>Objective.</h3> —To evaluate the impact of cotinine-confirmed smoking reduction during pregnancy on infant birth weight and gestational age at birth. <h3>Design.</h3> —Group analyses from a prospective, randomized smoking-cessation intervention trial using cotinine levels to assess smoking cessation and reduction. <h3>Setting.</h3> —Four maternity clinics of Jefferson County Health Department in Birmingham, Ala. <h3>Patients.</h3> —A total of 803 pregnant smokers and 474 never smokers with a fetal gestational age of 32 weeks or less at the first prenatal visit to a clinic. <h3>Main Outcome Measures.</h3> —Infant birth weight and gestational age at birth. <h3>Results.</h3> —Infants who were born to women who quit smoking (quitters) had the highest mean birth weight (3371 ±581 g), followed by infants who were born to women who reduced smoking (reducers) (3120±651 g), and infants who were born to women who did not change smoking behavior (no changers) (3043±587 g). The mean infant birth weight of infants born to the quitters, adjusted by mother's age, race, height, weight at baseline, and gestational age at delivery was 241 g heavier than that among the no changers (<i>P</i>=.0008) and 167 g heavier than the reducers (<i>P</i>=.04). The adjusted mean infant birth weight of infants born to the reducers was 92 g heavier than that among the no changers (<i>P</i>=.08). White reducers with baseline cotinine levels greater than 100 ng/mL had infants who were 241 g heavier than did white no changers. A 220-g difference was also seen in black reducers with a baseline cotinine level of 100 ng/mL or less. Although smoking cessation increased infant gestational age at delivery by 1 week, smoking reduction had little effect. <h3>Conclusion.</h3> —Continine-validated smoking reduction rates were positively associated with an increase in infant birth weight. While smoking cessation must continue to be the primary objective for pregnant smokers, specific intervention methods should also be directed toward smoking reduction for women who cannot quit. (<i>JAMA</i>. 1993;269:1519-1524)
Adherence of Catheterization Laboratory Cardiologists to American College of Cardiology/American Heart Association Guidelines for Percutaneous Coronary Interventions and Coronary Artery Bypass Graft Surgery
Authors: Edward L. Hannan, Michael J. Racz, Jeffrey Gold, Kimberly Cozzens, Nicholas J. Stamato, Tia Powell, Mary Hibberd, Gary Walford
Journal: Circulation (2010)

The American College of Cardiology and the American Heart Association have issued guidelines for the use of coronary artery bypass graft surgery (CABG) and percutaneous coronary interventions (PCI) for many years, but little is known about the impact of these evidence-based guidelines on referral decisions.A cardiac catheterization laboratory database used by 19 hospitals in New York State was used to identify treatment (CABG surgery, PCI, medical treatment, or nothing) recommended by the catheterization laboratory cardiologist for patients undergoing catheterization with asymptomatic/mild angina, stable angina, and unstable angina/non-ST-elevation myocardial infarction between January 1, 2005, and August 31, 2007. The recommended treatment was compared with indications for these patients based on American College of Cardiology/American Heart Association guidelines. Of the 16 142 patients undergoing catheterization who were found to have coronary artery disease, the catheterization laboratory cardiologist was the final source of recommendation for 10 333 patients (64%). Of these 10 333 patients, 13% had indications for CABG surgery, 59% for PCI, and 17% for both CABG surgery and PCI. Of the patients who had indications for CABG surgery, 53% were recommended for CABG and 34% for PCI. Of the patients with indications for PCI, 94% were recommended for PCI. For the patients who had indications for both CABG surgery and PCI, 93% were recommended for PCI and 5% for CABG surgery. Catheterization laboratory cardiologists in hospitals with PCI capability were more likely to recommend patients for PCI than hospitals in which only catheterization was performed.Patients with coronary artery disease receive more recommendations for PCI and fewer recommendations for CABG surgery than indicated in the American College of Cardiology/American Heart Association guidelines.
Mannitol Infusion
Authors: Kevin G. Barry, Arthur Cohen, James P. Knochel, Thomas J. Whelan, William R. Beisel, Cesar A. Vargas, Preston C. LeBlanc
Journal: New England Journal of Medicine (1961)

INCREASED longevity has been paralleled by a rise in the number of patients afflicted with aneurysms of the abdominal aorta. The survival outlook for these patients is bleak unless aneurysmectomy is performed.1 A satisfactory technical surgical result is too frequently nullified by fatal acute renal failure.2 3 4 Crossclamping of the abdominal aorta below the renal arteries and manipulation of the aorta appear causally related to the high incidence of renal failure associated with aneurysmectomy.5 , 6 Suggested possible mechanisms vary from atheromatous embolization to reflex renovascular spasm.3 , 7 In the laboratory animal, trauma applied to areas distant from the kidneys can cause reflex renovascular . . .
The Mechanics of Malabsorption.
Authors: F. J. Ingelfinger
Journal: Annals of Internal Medicine (1961)

s1 May 1961The Mechanics of Malabsorption.F. J. Ingelfinger, M.D., F.A.C.P.F. J. Ingelfinger, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-54-5-1049_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptNormal absorption depends initially on active digestion, and eventually on the presence of healthy mucosal cells. As an intermediate requirement, however, an adequate contact must be established between the products of digestion on the one hand, and a sufficient number of healthy and specifically endowed absorbing cells, on the other. When this contact is inadequate, absorption may suffer in proportion. Mechanisms responsible for preventing adequate contact may act singly or in combination:1. The area of absorbing surface is curtailed by ablation, by by-pass, or by a degenerative loss of villous projections. The effects of such curtailment are determined not... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: F. J. Ingelfinger, M.D., F.A.C.P.Affiliations: Boston, Mass. (ML) PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 May 1961Volume 54, Issue 5 Page: 1049-1049 Keywords Cells Digestion ePublished: 1 December 2008 Issue Published: 1 May 1961 PDF downloadLoading ...
Airway responsiveness to beta-agonist and beta2-adrenoceptor gene polymorphisms
Authors: K. Asano, Y. Suzuki, T. Shiomi, T. Nakajima, H. Kudoh, T. Matsuzaki, W. Yamada, R. Hiraoka, K. Yamaguchi, A. Nagabukuro, Y. Harada
Journal: Journal of Allergy and Clinical Immunology (2003)
Endotracheal Anesthesia for Laryngoscopy and Bronchoscopy
Authors: Thomas K. Lammert
Journal: JAMA (1961)

The MULTIPLICITY of methods suggested in the literature for anesthesia for laryngoscopy and bronchoscopy testifies to the failure to agree on any one good procedure. These endoscopic procedures—of their very nature—present a major problem: how to maintain the patient's respiration and airway safely while operating in the respiratory path.  The requirements for anesthesia for these procedures are (1) absence or minimization of pain for the patient, (2) an adequate degree of relaxation for the insertion and manipulation of the laryngoscope and bronchoscope, and (3) adequate maintenance of the respiration and airway to avoid anoxia or hypoxia.  These endoscopic procedures, in the past, have most often been performed under topical anesthesia—usually accompanied by varying degrees of sedation. All of us are familiar with the anxiety, coughing, straining, and struggling which—all too often—accompany laryngoscopy and bronchoscopy, even if topical anesthesia is adequate. The strain of holding the mouth open, the sensations of
Histological aspects of DNCB sensitization and challenge tests
Authors: A. HARTMAN, PH.J. HOEDEMAEKER, J.P. NATER
Journal: British Journal of Dermatology (1976)

Journal Article Histological aspects of DNCB sensitization and challenge tests Get access A. HARTMAN, A. HARTMAN Departments of Dermatology, State University, Groningen, the Netherlands Search for other works by this author on: Oxford Academic Google Scholar PH.J. HOEDEMAEKER, PH.J. HOEDEMAEKER Departments of Pathology, State University, Groningen, the Netherlands Search for other works by this author on: Oxford Academic Google Scholar J.P. NATER J.P. NATER Departments of Dermatology, State University, Groningen, the Netherlands Reprint requests: Dr J.P.Nater, Department of Dermatology, State University Hospital, Oostersingel 59, Groningen, The Netherlands. Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 94, Issue 4, 1 April 1976, Pages 407–416, https://doi.org/10.1111/j.1365-2133.1976.tb06118.x Published: 01 April 1976
Fetal Risks in Rubella Vaccination
Authors: Murray D. Kuhr
Journal: JAMA (1973)

To the Editor.—  Data of Wyll and Herrmann (225:1472, 1973) regarding fetal risk in inadvertent rubella vaccination might be interpreted differently. The first risk to the fetus is induced abortion; in this group (50%), the mortality rate was 100%. The outlook for the survivors of this risk is excellent; 11% were spontaneous abortions—a rate better than the spontaneous abortion rate in mothers who did not receive inadvertent rubella vaccination. In the cases resulting in live offspring there was not a single documented case of congenital rubella. All 11 viable offsprings had absence of viral isolation; 12 of 12 had normal cord immunoglobulin M levels; 39 of 39 had normal follow-up examinations at 4 to 20 months; and 96 of 96 had normal examinations at birth. The documented morbidity of inadvertent rubella vaccination early in gestation was nil among those not aborted, even in the susceptible group.  Among those aborted, attenuated
Hospital patients should be assessed for risk of thromboembolism
Authors: Susan Mayor
Journal: BMJ (2007)

Every hospital patient should be assessed for their risk of developing venous thromboembolism (VTE), an expert working group has recommended to the Department of Health in England.

The latest figures show that about 30 000 people die from venous thromboembolism a year in English hospitals. The government set up the expert working group to explore how best practice and guidance could be promoted and implemented to reduce the risk of venous thromboembolism.

The group recommends a mandatory documented assessment of the risk of the …
Your practice is you. Reflections of a diagnostician. An interview with B. Harvey Minchew, MD
Authors: B. H. Minchew
Journal: JAMA (1982)

<h3>A Physician's Son</h3> My father was a physician in Waycross, Ga. He was 50 years old when I was born, and at the time he practiced EENT (subsequent to that, he practiced ophthalmology, and before that he had been a general practitioner). I can remember making rounds with him to see children with diphtheria. At that time, there was little that could be done except to perform a tracheostomy and ask the hospital administrators to waive the visiting rules so that the parents could stay with the dying child. But my father enjoyed medicine, and I appreciated very early what satisfaction he got from his relationships with people. I remember comparing his occupation with those of my friends' fathers, and thinking that what he did was preferable. I can't think of anyone other than my father whom I have tried to emulate as a person. Although many people have had
The Global Implications of Influenza in Hong Kong
Authors: Marie R. Griffin, Kathleen M. Neuzil
Journal: New England Journal of Medicine (2002)

Was the disappearance of brooding storks in Germany during the latter half of the last century really responsible for the precipitous decline in the number of newborn babies there? This example illustrates why ecologic studies are considered among the weakest of epidemiologic methods. Nonetheless, in 1847, this relatively crude descriptive approach was sufficient to allow William Farr to show a strong correlation between the incidence of influenza-like illness and the annual excess of deaths in winter. Since then, public health practitioners have used variations on the ecologic approach to quantify the annual burden of influenza (Figure 1). After influenza A . . .
Ethylenediamine and piperazine sensitivity.
Authors: S Wright, R R Harman
Journal: BMJ (1983)

In this preliminary study we assessed the efficiency of glucagon given intranasally in raising blood glucose concentrations in normal subjects. Subjects and methodsSeven normal adult subjects, all doctors or undergraduate medical students at this hospital, volunteered for the study.They were studied while in bed in the morning, after fasting overnight, and not smoking.After 30 minutes to ensure steady blood glucose and plasma glucagon concentrations drops of solutions containing glucagon (1 mg/subject) were inserted in both nostrils and the subjects were asked to take a deep breath.In four subjects control experiments were performed on a different occasion and distilled water was substituted for glucagon, the subjects being unaware of the contents of the drops.Finally, in two of the above subjects glucagon 1 mg was given intramuscularly.Solutions to form nasal drops were obtained from porcine glucagon (Novo, Copenhagen, Denmark) diluted with sodium glycocholate 10 or 15 mg as a surfactant.5 Blood samples were obtained at the times indicated in the figure; placed in tubes containing heparin and aprotinin (10 000 KIU/ml), and centrifuged at 4'C.Blood glucose concentrations were estimated by an enzymatic method.Plasma was stored at -20'C until assayed for immuno- reactive insulin and immunoreactive glucagon.Immunoreactive glucagon was assayed using the antibody R-78, C-terminus directed.Incubation lasted 48 h at 4°C and free immunoreactive glucagon was separated from bound immunoreactive glucagon by centrifugation at 2000 g for 20 minutes.Kits for immunoreactive insulin and immunoreactive glucagon were supplied by Serono Biodata (Rome, Italy).Statistical analyses were performed by Student's t test for paired data and confirmed by the non-parametric Wilcoxon's test.1500
Stable Glomerular Filtration Rate in Normotensive IDDM Patients With Stable Microalbuminuria: A 5-year prospective study
Authors: Elisabeth R Mathiesen, Bo Feldt-Rasmussen, Eva Hommel, Torsten Deckert, Hans-Henrik Parving
Journal: Diabetes Care (1997)

OBJECTIVE To investigate the long-term course of glomerular filtration rate (GFR) in IDDM patients with microalbuminuria in order to identify patients with stable or declining kidney function over a 5-year study. RESEARCH DESIGN AND METHODS Forty normotensive (129 ± 11/80 ± 8 mmHg) IDDM patients with persistent microalbuminuria (mean urinary albumin excretion [UAE] 84 mg/24 h [range 30–300]) were followed prospectively for 5 years of clinical examinations that included the measurement of GFR (51Cr-labeled EDTA clearance) at least once a year. The mean GFR at baseline was 120 ± 18 ml · min−1 · 1.73 m−2. RESULTS Using multiple regression analysis, the rate of decline in GFR was independently correlated to onset of diabetic nephropathy (P &amp;lt; 0.001) and systolic blood pressure (sBP) at baseline (P &amp;lt; 0.05). Increase in UAE was correlated to the mean HbA1c during the observation period. Out of 40 patients, 14 progressed to diabetic nephropathy (UAE &amp;gt; 300 mg/24 h). These patients had a significant reduction in GFR (mean −2.2 ± 3.8 ml · min−1 · year−1; P = 0.05), while GFR remained stable in the remaining 26 patients with nonprogressive microalbuminuria (change in GFR 0.5 ± 2.1 ml · min−1 · year−1; NS). The difference in the rate of decline of GFR was significant (mean 2.7 ml · min−1 · year−1; P &amp;lt; 0.05). CONCLUSIONS Normotensive IDDM patients with nonprogressive microalbuminuria have a stable GFR. Progression of UAE to diabetic nephropathy heralds a progressive loss of kidney function. Efforts should be made to prevent the progression from microalbuminuria to diabetic nephropathy in every IDDM patient with microalbuminuria.