tbuckley/cabot-search huggingface dataset:
Abstract 2483: Transgenic mice to evaluate the role of S-adenosylmethionine decarboxylase in skin carcinogenesis
Authors: Chenxu Shi, Diane E. McCloskey, Anthony E. Pegg, David J. Feith
Journal: Cancer Research (2010)

Abstract To evaluate the role of S-adenosylmethionine decarboxylase (AdoMetDC) and altered polyamine levels in mouse skin carcinogenesis, we utilized the tetracycline-regulated system to produce conditional expression of AdoMetDC in basal keratinocytes. AdoMetDC is a key enzyme in polyamine biosynthesis and it catalyzes the decarboxylation reaction converting AdoMet to dcAdoMet. dcAdoMet provides the aminopropyl groups for the synthesis of the higher polyamines spermidine and spermine from the precursor putrescine. Therefore, elevated AdoMetDC activity may promote the synthesis of higher polyamines at the expense of putrescine, and increased putrescine levels are tightly linked to tumor promotion in mouse skin. AdoMetDC activity is also known to be induced by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). To make the DNA construct for microinjection, human AdoMetDC cDNA was inserted into the TetO vector followed by an internal ribosome entry site and luciferase coding sequence. Potential founder mice were crossed to mice with skin-specific expression of tetracycline-regulated transcriptional activator (tTA) directed by bovine keratin 5 (K5) promoter elements to generate K5-tTA/TetO-AdoMetDC bitransgenic mice. Transgene expression has been characterized by in vivo bioluminescent imaging of luciferase activity, in vitro luciferase activity as well as AdoMetDC activity assay and Western blotting in 7 week old mice. In bitransgenic mice, AdoMetDC is constitutively expressed in the skin and expression can be repressed by exposure to the tetracycline analogue doxycycline (Dox). In untreated bitransgenic mice generated from two founder lines, we saw a massive increase in luciferase activity and these mice have a thin fur phenotype. Minimal transgene expression is detected in either single transgenic TetO-AdoMetDC or Dox-treated bitransgenic mice. Untreated bitransgenic mice from one founder line exhibit a 7.2- fold increase in AdoMetDC activity in epidermis, a 7.8-fold increase in dermis, and dcAdoMet levels are also elevated by 8.0-fold in dermis. Effects of AdoMetDC overexpression on TPA induced polyamine biosynthesis and susceptibility to 7,12-dimethylbenz(a)anthracene/TPA carcinogenesis are now being investigated. These mice with regulated AdoMetDC expression in the skin provide a valuable model system to evaluate the role of AdoMetDC and polyamines in skin carcinogenesis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2483.
Healthy environments for healthy people: bioremediation today and tomorrow.
Authors: C Bonaventura, F M Johnson
Journal: Environmental Health Perspectives (1997)

Increases in environmental contamination lead to a progressive deterioration of environmental quality. This condition challenges our global society to find effective measures of remediation to reverse the negative conditions that severely threaten human and environmental health. We discuss the progress being made toward this goal through application of bioremediation techniques. Bioremediation generally utilizes microbes (bacteria, fungi, yeast, and algae), although higher plants are used in some applications. New bioremediation approaches are emerging based on advances in molecular biology and process engineering. Bioremediation continues to be the favored approach for processing biological wastes and avoiding microbial pathogenesis. Bioremediation may also play an increasing role in concentrating metals and radioactive materials to avoid toxicity or to recover metals for reuse. Microbes can biodegrade organic chemicals; purposeful enhancement of this natural process can aid in pollutant degradation and waste-site cleanup operations. Recently developed rapid-screening assays can identify organisms capable of degrading specific wastes and new gene-probe methods can ascertain their abundance at specific sites. New tools and techniques for use of bioremediation in situ, in biofilters, and in bioreactors are contributing to the rapid growth of this field. Bioremediation has already proven itself to be a cost-effective and beneficial addition to chemical and physical methods of managing wastes and environmental pollutants. We anticipate that it will play an increasingly important role as a result of new and emerging techniques and processes.
THE PHYSIOLOGIC BASIS OF THE MANIC-DEPRESSIVE ILLNESS: A THEORY
Authors: S. H. KRAINES
Journal: American Journal of Psychiatry (1957)

Clinical experience and studies of heredity indicate that the etiology of the manic-depressive illness is physiologic. Examination of the possible sites of the physiopathology implicates diencephalic-rhinencephalic-reticular brain systems. It is the secondary involvement of the cerebral cortex that results in psychopathology. Psychodynamic mechanisms use the patient's experiences and reaction patterns in the formation of symptoms. As the physiopathology spontaneously improves, so do the symptoms.
Using Neurobiological Measures to Predict and Assess Treatment Outcome of Psychotherapy in Posttraumatic Stress Disorder: Systematic Review
Authors: Jasper B. Zantvoord, Julia Diehle, Ramón J.L. Lindauer
Journal: Psychotherapy and Psychosomatics (2013)

&lt;b&gt;&lt;i&gt;Background:&lt;/i&gt;&lt;/b&gt; Trauma-focused cognitive-behavioral therapy (TF-CBT) and eye movement desensitization and reprocessing (EMDR) are effective treatments for posttraumatic stress disorder. However, little is known about their neurobiological effects. The usefulness of neurobiological measures to predict the treatment outcome of psychotherapy also has yet to be determined. &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; Systematic review of randomized controlled trials (RCTs) focused on neurobiological treatment effects of TF-CBT or EMDR and trials with neurobiological measures as predictors of treatment response. &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; We included 23 publications reporting on 16 separate trials. TF-CBT was compared with a waitlist in most trials. TF-CBT was associated with a decrease in heart rate and blood pressure and changes in activity but not in volume of frontal brain structures and the amygdala. Neurobiological changes correlated with changes in symptom severity. EMDR was only tested against other active treatments in included trials. We did not find a difference in neurobiological treatment effects between EMDR and other treatments. Publications on neurobiological predictors of treatment response showed ambiguous results. &lt;b&gt;&lt;i&gt;Conclusion:&lt;/i&gt;&lt;/b&gt; TF-CBT was associated with a reduction of physiological reactivity. There is some preliminary evidence that TF-CBT influences brain regions involved in fear conditioning, extinction learning and possibly working memory and attention regulation; however, these effects could be nonspecific psychotherapeutic effects. Future trials should use paradigms aimed specifically at these brain regions and physiological reactivity. There are concerns regarding the risk of bias in some of the RCTs, indicating that methodologically more rigorous trials are required. Trials with neurobiological measures as predictors of treatment outcome render insufficient results to be useful in clinical practice.
Gray platelet syndrome and defective thrombo-inflammation in Nbeal2-deficient mice
Authors: Carsten Deppermann, Deya Cherpokova, Paquita Nurden, Jan-Niklas Schulz, Ina Thielmann, Peter Kraft, Timo Vögtle, Christoph Kleinschnitz, Sebastian Dütting, Georg Krohne, Sabine A. Eming, Alan T. Nurden, Beate Eckes, Guido Stoll, David Stegner, Bernhard Nieswandt
Journal: Journal of Clinical Investigation (2013)

Platelets are anuclear organelle-rich cell fragments derived from bone marrow megakaryocytes (MKs) that safeguard vascular integrity. The major platelet organelles, α-granules, release proteins that participate in thrombus formation and hemostasis. Proteins stored in α-granules are also thought to play a role in inflammation and wound healing, but their functional significance in vivo is unknown. Mutations in NBEAL2 have been linked to gray platelet syndrome (GPS), a rare bleeding disorder characterized by macrothrombocytopenia, with platelets lacking α-granules. Here we show that Nbeal2-knockout mice display the characteristics of human GPS, with defective α-granule biogenesis in MKs and their absence from platelets. Nbeal2 deficiency did not affect MK differentiation and proplatelet formation in vitro or platelet life span in vivo. Nbeal2-deficient platelets displayed impaired adhesion, aggregation, and coagulant activity ex vivo that translated into defective arterial thrombus formation and protection from thrombo-inflammatory brain infarction following focal cerebral ischemia. In a model of excisional skin wound repair, Nbeal2-deficient mice exhibited impaired development of functional granulation tissue due to severely reduced differentiation of myofibroblasts in the absence of α-granule secretion. This study demonstrates that platelet α-granule constituents are critically required not only for hemostasis but also thrombosis, acute thrombo-inflammatory disease states, and tissue reconstitution after injury.
Intractable Ventricular Tachycardia Due to Ventricular Aneurysm with Surgical Cure
Authors: EMIL R. RITTER
Journal: Annals of Internal Medicine (1969)

A case of intractable ventricular tachycardia associated with a post-infarction ventricular aneurysm is presented. Conversion with drug agents and electroshock were either only briefly successful or unsuccessful. Finally, after 9 days of persistent ventricular tachycardia, resection of the aneurysm was carried out with excellent eventual recovery and correction of ventricular tachycardia. Awareness of this association and its possible surgical correction is stressed. Similar cases may become more frequent since improved coronary care will probably result in more infarction survivors with aneurysms.
Counting our blessings
Authors: Theodore Dalrymple
Journal: BMJ (2008)

It is an old adage that we should count our blessings, and it is an equally ancient failing of human beings that they should fail to do so. For we are as much problem seeking as problem solving creatures, and we soon feel wretched if we have nothing to complain about.

My wife and I once visited a missionary doctor in Haiti called Dr Hodges. I had met him there 10 years earlier, when he told me that he wished to retire soon in Haiti and concentrate on archaeology. (He had discovered the place where Christopher Columbus had first landed and founded a small museum.) But when we met him again the medical …
THE TREATMENT OF OSTEOMYELITIS WITH IODOFORM BONE PLUGGING.
Authors: GEORGE MORRIS DORRANCE
Journal: JAMA (1906)

The usual methods employed in the treatment of osteomyelitis had given such poor results in my own and in the work of others that I was led to try the iodoform plugging recommended by Moorhof. The methods of treating osteomyelitis are practically the same up to the point of filling the cavity. The methods of filling the cavity are as follows: With iodoform gauze. This has given very poor results, usually requires numerous operations and too frequently amputation of the limb. Senn's method of filling the cavity with decalcified bone chips dusted with iodoform powder and allowing it to fill with blood clot. In this method the chips are frequently extruded and the cavity usually becomes infected. Schede's method of filling the cavity with blood clot has had some success, but the clot very frequently becomes infected and the patient is worse than before the operation. Neuber's method of tacking
Breast Cancer Screening With Tomosynthesis and Digital Mammography
Authors: David Seidenwurm, Robert Rosenberg
Journal: JAMA (2014)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Treatment of acute hypernatremia in severely burned patients using continuous veno–venous hemofiltration with gradient sodium replacement fluid: a report of nine cases
Authors: Chen Huang, Peng Zhang, Rui Du, Yangping Li, Yan Yu, Meilan Zhou, Rui Jing, Li Li, Yong Zheng, Hanmin Wang, Hongbao Liu, Lijie He, Shiren Sun
Journal: Intensive Care Medicine (2013)
HO-1–STAT3 axis in mouse liver ischemia/reperfusion injury: Regulation of TLR4 innate responses through PI3K/PTEN signaling
Authors: Bibo Ke, Xiu-Da Shen, Haofeng Ji, Naoko Kamo, Feng Gao, Maria Cecilia S. Freitas, Ronald W. Busuttil, Jerzy W. Kupiec-Weglinski
Journal: Journal of Hepatology (2011)
Role of minimal residual disease monitoring in acute promyelocytic leukemia treated with arsenic trioxide in frontline therapy
Authors: Ezhilarasi Chendamarai, Poonkuzhali Balasubramanian, Biju George, Auro Viswabandya, Aby Abraham, Rayaz Ahmed, Ansu Abu Alex, Saravanan Ganesan, Kavitha M. Lakshmi, Usha Sitaram, Sukesh Chandran Nair, Mammen Chandy, Nancy Beryl Janet, Vivi M. Srivastava, Alok Srivastava, Vikram Mathews
Journal: Blood (2012)
Critical regulation of CD4+ T cell survival and autoimmunity by β-arrestin 1
Authors: Yufeng Shi, Yan Feng, Jiuhong Kang, Chang Liu, Zhenxin Li, Dangsheng Li, Wei Cao, Ju Qiu, Zhengliang Guo, Enguang Bi, Lei Zang, Chuanzhen Lu, Jingwu Z Zhang, Gang Pei
Journal: Nature Immunology (2007)
Nuclear receptor/microRNA circuitry links muscle fiber type to energy metabolism
Authors: Zhenji Gan, John Rumsey, Bethany C. Hazen, Ling Lai, Teresa C. Leone, Rick B. Vega, Hui Xie, Kevin E. Conley, Johan Auwerx, Steven R. Smith, Eric N. Olson, Anastasia Kralli, Daniel P. Kelly
Journal: Journal of Clinical Investigation (2013)

The mechanisms involved in the coordinate regulation of the metabolic and structural programs controlling muscle fitness and endurance are unknown. Recently, the nuclear receptor PPARβ/δ was shown to activate muscle endurance programs in transgenic mice. In contrast, muscle-specific transgenic overexpression of the related nuclear receptor, PPARα, results in reduced capacity for endurance exercise. We took advantage of the divergent actions of PPARβ/δ and PPARα to explore the downstream regulatory circuitry that orchestrates the programs linking muscle fiber type with energy metabolism. Our results indicate that, in addition to the well-established role in transcriptional control of muscle metabolic genes, PPARβ/δ and PPARα participate in programs that exert opposing actions upon the type I fiber program through a distinct muscle microRNA (miRNA) network, dependent on the actions of another nuclear receptor, estrogen-related receptor γ (ERRγ). Gain-of-function and loss-of-function strategies in mice, together with assessment of muscle biopsies from humans, demonstrated that type I muscle fiber proportion is increased via the stimulatory actions of ERRγ on the expression of miR-499 and miR-208b. This nuclear receptor/miRNA regulatory circuit shows promise for the identification of therapeutic targets aimed at maintaining muscle fitness in a variety of chronic disease states, such as obesity, skeletal myopathies, and heart failure.
A Statewide Plan for Care of the Poisoned Patient: The Massachusetts Poison Control System
Authors: Frederick H. Lovejoy, Debra L. Caplan, Thomas Rowland, Louis Fazen
Journal: New England Journal of Medicine (1979)

An estimated five million poisonings occur in the United States each year, causing over 5000 deaths. As a cause of accidental death, poisonings are surpassed only by motor-vehicle accidents, drownings and burns.1 This problem did not begin to attract sufficient attention to initiate professional and legislative activity until the late 1940's and early 1950's.2 As a result of the Cooperative efforts of the Illinois Chapter of the American Academy of Pediatrics, the Illinois State Health Department and seven major hospitals, the first poison control center was established in 1953 as a pilot project in Chicago.3 Others followed, and the poison . . .
Enumeration and identification of airborne viable mould propagules in houses
Authors: A. P. Verhoeff, J. H. van Wijnen, J. S. M. Boleij, B. Brunekreef, E. S. van Reenen‐Hoekstra, R. A. Samson
Journal: Allergy (1990)

A number of techniques for the enumeration and identification of viable mould propagules in the indoor air of houses were evaluated in order to document to what extent different results are obtained when different methods are used. A comparison was made between the results obtained with five commercially available air sampling devices (Slit‐to agar sampler, N6‐Andersen sampler, Surface Air System sampler, Reuter Centrifugal Air sampler. Gelatine Filter sampler) and a non‐volumetric sampler (the Open Petri Dish), in combination with four culture media (malt extract agar, dichloran glycerol‐18 agar, oxytetracycline glucose yeast extract agar and dichloran rose bengal chloramphenicol agar). The coefficients of variation were high (generally &lt; 20%) for all combinations. Statistical analysis showed that the Slit sampler and the N6‐Andersen sampler in combination with DG18 and MEA gave the best precision and the highest yield in terms of colony forming units per square cubic meter of air (CFU/m 3 ) and number of species isolated.
Role of the Anesthesiologist
Authors: James F. Mayhew
Journal: JAMA (1982)

<h3>To the Editor.—</h3> We empathize with T. Cameron MacCaughelty, MD, in his article "An Anesthesiologist's Plea" (1981;246:2692). However, we came to the conclusion years ago that we could not legislate respectability regardless of how hard we or our national organization tried. Regardless of the situation, we have found it the same. Even though we have suggested or have made interventions that have shown both our expertise and our interest in the individual patient's problems and care and have demonstrated by that intervention an improvement in care, we have found later only to be considered a "hand-maiden" to the other physicians involved with the patient. Yes, Dr MacCaughelty, we do have much to offer our patients and do look forward to improved rapport with our physician colleagues, but like you, we have not found the answer despite our continued presence in both the operating room and intensive care areas.
Laboratory Evaluation of New Reusable Blood Glucose Sensor
Authors: Stuart J Updike, Mark C Shults, Chris C Capelli, Don von Heimburg, Rathbun K Rhodes, Nilufer Joseph-Tipton, Brian Anderson, David D Koch
Journal: Diabetes Care (1988)

An enzyme-electrode sensor designed specifically for pocket-portable self-monitoring of blood glucose is described. The sensing device in this instrument is unique because it is reusable for at least 30 days, at which time it is easily replaced by placing a new enzyme-membrane cartridge over the electrode. As little as 7 μl of undiluted whole blood, plasma, or serum is applied directly to the sensor, and glucose is automatically determined in 30 s. No manual timing or wiping step is required after sample application. On eight production instruments, plasma glucose concentration was determined (n = 20) at 57, 125, 246, and 347 mg/dl. The average coefficient of variation for the 80 determinations for each instrument ranged from 2 to 5%, averaging 3.7%. The instrument is inherently linear, independent of hematocrit, and without oxygen limitation when dissolved oxygen concentration is &amp;gt;35 mmHg. No interferences were found from plasma constituents, heparin, or acetaminophen.
METABOLISM OF WOMEN DURING THE REPRODUCTIVE CYCLE. XVII. CHANGES IN ELECTROPHORETIC PATTERNS OF PLASMA PROTEINS THROUGHOUT THE CYCLE AND FOLLOWING DELIVERY 12
Authors: Margaret N. Coryell, Eliot F. Beach, Abner R. Robinson, Icie G. Macy, Harold C. Mack
Journal: Journal of Clinical Investigation (1950)

The present investigation, an electrophoretic study of blood proteins, was planned to obtain information concerning the preconceptional physi- ologic status of women, the physiologic changes in the maternal organism in preparation for the exi- gencies of labor and losses at delivery, for the readjustment during the puerperium, and the de- mands of lactation.The results of this study will provide the basis for a better understanding of electrophoretic determinations of the proteins in the blood of women whose child-bearing was com- plicated by abnormal conditions or disease (1).While this investigation was in progress (2) the Tiselius procedure (3) for electrophoretic sep- aration of blood proteins was applied to maternal, fetal, and infant sera by Longsworth, Curtis and Pembroke (4), Lagercrantz (5), Moore, DuPan and Buxton (6), and Scrimshaw and Alling (7) in studies of placental permeability and toxemias of pregnancy but, to our knowledge, this is the first report of electrophoretic determinations of constituents of the blood of healthy women throughout the reproductive cycle, from the preconcep- tional state through the postpartum readjustment period.The observations on cord blood will be presented in another communication. METHODS AND MATERIALSHealthy, well-nourished women in the early stages of gestation were selected from among his private patients
Letter: Management of acute asthma.
Authors: K B Saunders
Journal: BMJ (1975)

visit Should the first level be above 7 mnnIl/l (270 mg/100 ml) or any subse- quent value exceed this figure, then the pill should normally not be commenced or con- tinued.But even where there are no facilities for such analyses the well-trained presmiber, whether medical or non-medical, and the fully informed user should be able to balance the dangers of the pill against its benefits in each individual case.
Is the brain specified?
Authors: Jean Pailhous, Elodie Varraine, Mireille Bonnard
Journal: Behavioral and Brain Sciences (2001)

How to conceive the place of the brain in the specification of the animal environment relation? Reality is a continuum between external physical energies and brain energy. The global array concept linked to the physical world and its physical energies could be transposed to the brain as a physical object and a dynamical system.