tbuckley/cabot-search huggingface dataset:
712. High Capacity Screen To Select Optimal Pre-Trans-Splicing Molecules for Trans-Splicing Applications
Authors: M. Puttaraju, Yanping Yang, Jonathan E. Spindler, Jun Wang, Colette A. Cote, Weng Ke, Garry P. Nolan, S. Gary Mansfield, Lloyd G. Mitchell, Edward Otto
Journal: Molecular Therapy (2004)

Spliceosome Mediated RNA Trans-splicing (SMaRT™) is a platform technology to reprogram genes at the pre-mRNA level. Applications include RNA therapy and real-time molecular imaging, with each application dependent upon the nature of the sequences encoded by the pre-trans-splicing molecule (PTM). SMaRT utilizes native spliceosomes to carry out trans-splicing reactions between two RNA molecules: a pre-mRNA target present in the cell and a delivered PTM.
Equestrienne on Horse
Authors: M. Therese Southgate
Journal: JAMA (2003)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 19994: No Mortality Advantage of “On” versus “Off”-hours Primary Percutaneous Coronary Intervention for ST-Elevation Myocardial Infarction: Results from the Massachusetts Data Analysis Center Registry
Authors: Roberto Cubeddu, Rodrigo Lago, Ivan Mendoza, Ann F Lovett, Katya Zelevinsky, Ashley M Crane, Christian Witzke, Sharon-Lise Normand, Eugene Pomerantsev, Igor F Palacios
Journal: Circulation (2010)

Background:  A higher mortality rate has been reported in STEMI admissions during off-hours due to lower availability of primary percutaneous coronary intervention (PCI) and longer door-to-balloon-times (DTB). The data however remains controversial, and is non-applicable to a wide range of hospitals that routinely perform primary PCI. The purpose of this study was to establish whether STEMI admissions undergoing primary PCI have worse in-hospital outcomes during off-hours.   Methods:  Using the large, mandatory state-wide database of Massachusetts with consistently audited outcomes, we identified all STEMI patients who between 2006 and 2008 were admitted for primary PCI to an acute care hospital facility where primary PCI is routinely performed. Patients admitted during on-hours where compared to off-hours, which included weekends and holidays. Transfer patients or those previously treated with thrombolytics were excluded. The outcome of in-hospital mortality, cardiogenic shock and recurrent MI was examined.   Results:  Our cohort included 3892 patients (42% on-hours vs. 58% off-hours) with a mean age of 61±13 years. Demographic characteristics were similar between on- and off-hours groups. Overall, among off- and on-hours respectively, there were no differences in the incidence of in-hospital death (p=0.66), recurrent MI (p=0.11), and cardiogenic shock (p=0.84) between both groups. However, off-hours STEMI admissions had significantly longer mean DTB (87.6 min vs. 68.4 min, p   Conclusions:  Our findings, based on large registry data demonstrate that despite longer DTB times, similar rates of in-hospital death, recurrent MI, and periprocedural cardiogenic shock may be expected in STEMI patients admitted during off-hours when primary PCI is performed
Not So Fast: Research on Infectious Links to MS Questioned
Authors: B Vastag
Journal: JAMA (2001)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract P430: Spatial Proximity and Membership to Gyms or Related Facilities Jointly Predict Objectively Measured Physical Activity among Adults in New York City
Authors: Tanya K Kaufman, Daniel Sheehan, Kathryn M Neckerman, Andrew Rundle, Gina S Lovasi
Journal: Circulation (2014)

Background: Over half of the adult population in the United States fails to meet public health recommendations for physical activity. Neighborhood physical activity facilities may help encourage moderate and vigorous physical activity. We hypothesize that commercial physical activity facilities near the home would predict both membership in a gym or other recreation facility in the past 12 months and current physical activity. We further hypothesize the presence of effect modification, such that physical activity facilities would have a stronger association with activity among individuals with a facility membership. Methods: Data were from the New York City Physical Activity and Transit Monitoring Study, a sample of 679 New York City adults aged 18 years and older with physical activity measured by accelerometer. Participants were excluded for incomplete data, extreme values for height, weight or BMI, or if their home address could not be geocoded. The final analytic sample was 625. Counts of commercial physical activity facilities within 1 km of each participant’s home address were generated from the National Establishment Time-Series data for year 2010. Linear and logistic regression models incorporated robust standard errors, sample weights, and adjustment for individual- and neighborhood-level characteristics. Results: Individuals living near more commercial physical activity facilities were more likely to report membership in a gym or other facility (adjusted odds ratio for top versus bottom quartile of facility count: 3.80; 95% CI: 1.60-9.02). The count of facilities was also associated with more physical activity as measured by accelerometer, particularly for those individuals reporting membership in a gym or other recreation facility (see figure). Conclusion: The evaluation of opportunities for physical activity should include accessibility of commercial physical activity facilities, including financial or social barriers to membership.
Genomics and Proteomics May Help Clinicians Individualize Cancer Treatment
Authors: M J Friedrich
Journal: JAMA (2002)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
416. Transient Gene Expression by Non-Integrating Lentiviral (NIL) Vectors
Authors: Sarah Nightingale, Roger P. Hollis, Catherine Yang, Ingrid Bahner, Karen A. Pepper, Donald B. Kohn
Journal: Molecular Therapy (2004)

The ability to express genes transiently in stem cells would have a wide range of useful applications; for example the introduction of genes that could stimulate cell division, induce commitment to pathways of differentiation, or provide transient resistance to chemotherapeutic agents. Despite the potential usefulness of transient gene expression in hematopoietic stem cells (HSCs), there are still few methods that achieve gene delivery to these cells at high levels. Vectors based on attenuated Retroviruses and Lentiviruses have been used to efficiently and stably introduce genes into cells for long term expression. Their ability to stably transduce cells relies on the fact that their genome encodes an integrase protein that specifically recognizes sequences in the viral long terminal repeats (LTRs) and catalyses their insertion into host chromosomes. Due to their high efficiency of gene delivery and relatively low toxicity, we hypothesize that Non-Integrating Lentiviral (NIL) vectors may also be extremely useful in situations where transient gene expression is required. NIL vectors are lentiviral vectors harboring combinations of mutations made to disable the integrase protein itself and to alter the integrase recognition sequences located in the viral LTRs. Therefore, NIL vectors are designed to disable integration without affecting entry into cells, thus promoting transient gene expression. We have used these NIL vectors to transduce both HSCs and cell lines in order to assess their efficacy, as measured through transient eGFP expression. Our data demonstrate that transductions using the most effective NIL vectors harboring both LTR and integrase mutations resulted in initial high level transient eGFP expression (~90%), diminishing towards background in <1 month. This is in contrast to the wild-type vector which causes continued expression of eGFP (up to 3 months in cell lines). Encouragingly, NIL vectors performed equally well in primary human CD34+ cells as they did in cell lines. Southern blot analyses of transduced Jurkat cells depicted the loss of detectable NIL vector sequence after 1 month. Additionally the Southern analyses revealed important information regarding the frequency of formation of 1 or 2 LTR circles in comparison to linear DNA, which are typical molecular species formed by such non-integrating viral vectors. Through PCR and sequencing we have studied the number and nature of integrations of the NIL vectors in pools and colonies of Jurkat cells and compared this information to results from Jurkat cells exposed to the wild-type lentiviral vector. We believe that these studies may lead to the development of a vector system capable of highly efficient, but transient gene expression in human stem and mature cells.
Abstract 12781: NF-κB Activity is Required for Heart Regeneration
Authors: Ravi Karra, Anne Knecht, Kenneth D Poss
Journal: Circulation (2014)

Objectives: In contrast to humans, zebrafish recover from cardiac injury through a robust regenerative response. Recent work suggests that the mammalian heart is able to undergo low-grade cardiomyocyte turnover in response to injury. However, this level of turnover in not sufficient for cardiac recovery. A better understanding of the mechanisms that contribute to zebrafish heart regeneration can instruct approaches to achieve therapeutic heart regeneration in humans. Methods and Results: We performed expression profiling of regenerating zebrafish hearts by RNA-Seq to identify factors that modify heart regeneration. Expression levels for members of the NF-κB (nuclear factor κ-light-chain-enhancer of activated B cells) pathway were significantly enriched in regenerating hearts. Using a transgenic reporter strain for NF-κB activity, we found NF-κB to be induced in cardiomyocytes following injury. Moreover, NF-κB activity overlaps with the activation of gata4 regulatory sequences that are induced in regenerating cardiomyocytes. We subsequently developed a transgenic zebrafish strain to conditionally inhibit NF-κB signaling in cardiomyocytes by expression of mutant IκBα. Zebrafish hearts with loss of NF-κB activity scar following injury, indicative of impaired regeneration. Conclusions: NF-κB mediates an early transcriptional response to injury in cardiomyocytes that is required for heart regeneration.
Biodegradable Porous Hydrogels
Authors: Martin Pradny, Miroslav Vetrik, Martin Hruby, Jiri Michalek
Journal: Advanced Healthcare Materials (2014)

This chapter contains sections titled: Introduction Methods of Preparation of Porous Hydrogels Hydrogels Crosslinked With Degradable Crosslinkers Hydrogels Degradable in the Main Chain Conclusions Acknowledgments
Medical education in the United States
Authors: S. I. Etzel
Journal: JAMA (1970)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Acute exacerbations
Authors: Graeme P Currie, Jadwiga A Wedzicha
Journal: BMJ (2006)

<h3>Introduction</h3> African, Caribbean and Black (ACB) communities are disproportionately infected by HIV in Ontario, Canada. They constitute only 5% of the population of Ontario yet account for 25% of new diagnoses of HIV. The aim of this study is to understand underlying factors that augment the HIV risk in ACB communities and to inform policy and practice in Ontario. <h3>Methods and analysis</h3> We will conduct a cross-sectional study of first-generation and second-generation ACB adults aged 15–64 in Toronto (n=1000) and Ottawa (n=500) and collect data on sociodemographic information, sexual behaviours, substance use, blood donation, access and use of health services and HIV-related care. We will use dried blood spot testing to determine the incidence and prevalence of HIV infection among ACB people, and link participant data to administrative databases to investigate health service access and use. Factors associated with key outcomes (HIV infection, testing behaviours, knowledge about HIV transmission and acquisition, HIV vulnerability, access and use of health services) will be evaluated using generalised linear mixed models, adjusted for relevant covariates. <h3>Ethics and dissemination</h3> This study has been reviewed and approved by the following Research Ethics Boards: Toronto Public Health, Ottawa Public Health, Laurentian University; the University of Ottawa and the University of Toronto. Our findings will be disseminated as community reports, fact sheets, digital stories, oral and poster presentations, peer-reviewed manuscripts and social media.
Abstract 20089: Comparative Efficacy and Safety of Prasugrel and Clopidogrel in Patients With STEMI Undergoing Primary PCI in the Prasugrel Core Population in Clinical Practice. Results of the Prospective ALKK-registry
Authors: Uwe Zeymer, Matthias Hochadel, Anselm Gitt, Ralf Zahn
Journal: Circulation (2014)

Background:  In the TRITON-TIMI 38 trial prasugrel reduced the combined endpoint of cardiovascular death, myocardial infarction in patients with STEMI without an increase in bleeding complications. Therefore we evaluated the impact of therapy with prasugrel on outcome in patients with primary PCI for STEMI in real life in a large number of patients in the so-called prasugrel core population.   Methods:  We used the data of the ongoing prospective German ALKK-PCI registry and included patients with PCI for STEMI    Results:  Between 2009 and 2012 a total of consecutive 6227 patients with PCI for STEMI Of these 1921 (30.8 %) were treated with prasugrel. Baseline characteristics, procedural features and in-hospital outcomes are given in the table.  In a multivariate analysis prasugrel was associated with a reduced mortality (odds ratio 0.66, 95% CI 0.44-0.99).   Conclusion:  In clinical practice prasugrel compared to clopidogrel in the so-called core population is associated with a lower use of GP IIb/IIIa inhibitors and a lower mortality in patients with STEMI undergoing primary PCI. These findings support the findings of the TRITON-TIMI 38 trial.
Abstract P217: Reduced Lipoprotein-Associated Phospholipase A2 Activity Levels are Linked to Apolipoprotein C3 Loss-of-Function Mutations: The Atherosclerosis Risk in Communities Study
Authors: Yashashwi Pokharel, Wensheng Sun, Linda Polfus, Aaron Folsom, Gerardo Heiss, Richey Sharrett, Christie Ballantyne, Ron Hoogeveen
Journal: Circulation (2015)

Introduction: Higher lipoprotein-associated phospholipase A2 (LpPLA2) levels are associated with higher CHD risk. LpPLA2 is primarily transported in LDL particles and was shown to be associated positively with LDL-C but negatively with HDL-C levels. The mechanism for the negative association with HDL-C is unclear. One hypothesis is that LpPLA2 is increased in small dense LDL (sdLDL) generated with delayed clearance of lipoproteins as exhibited in atherogenic dyslipidemia. Apolipoprotein C3 (ApoC3) was shown to inhibit lipoprotein lipase’s lipolytic activity. ApoC3 loss-of-function (LOF) mutations are associated with lower triglycerides and sdLDL levels and higher HDL-C levels, reduced postprandial lipemia and reduced CHD risk. However, the association of ApoC3 LOF mutations with LpPLA2 activity is not known. We hypothesized that LpPLA2 activity levels will be lower with ApoC3 LOF mutation. Methods: In 4453 individuals of European ancestry in the Atherosclerosis Risk in Communities (ARIC) study we examined...
ABC of oxygen: Provision of oxygen at home
Authors: P J Rees, F Dudley
Journal: BMJ (1998)

Before patients in the United Kingdom are given long term oxygen therapy at home they must have arterial blood gas analysis, which is done at a hospital laboratory. The assessment should be supervised by a respiratory physician. In England and Wales this conflicts with the need for home oxygen to be prescribed by the patient's general practitioner. In Scotland arrangements are made by the respiratory specialist. Close liaison between general practice and hospital is necessary to ensure appropriate care for the patient. Often this is best organised by respiratory specialist nurses and practice nurses.



Patient using oxygen at home



The assessments should be made with the patient in a stable state on optimal treatment and must be confirmed by a second assessment at least three weeks later. Several studies show that results from a single estimation are not always confirmed by a repeat analysis 4-12 weeks later. Although the UK criteria specify an arterial carbon dioxide pressure (PaCO2) >6 kPa for treatment, it is accepted that patients with a lower PaCO2 should have the same treatment. Criteria in other countries differ slightly.

View this table:

Criteria for long term oxygen in different countries



The aim of long term oxygen therapy is to maintain arterial oxygen pressure (PaO2) at 9.3-12 kPa. This is usually achieved by a flow rate of 1-3 l/min. Ideally the PaO2 should be over 10.7 kPa to allow for falls during exercise, eating, sleeping, etc.

#### Prescription of long term oxygen in chronic obstructive pulmonary disease

Most doctors would not prescribe home oxygen for people who continue to smoke. The evidence of …
Abstract 711: Salmonella typhimurium A1-R induces quiescent FUCCI-expressing cancer cells to cycle and become chemosensitive
Authors: Shuya Yano, Yong Zhang, Ming Zhao, Yukihiko Hiroshima, Shinji Miwa, Fuminari Uehara, Hiroyuki Kishimoto, Hiroshi Tazawa, Toshiyoshi Fujiwara, Robert M. Hoffman
Journal: Cancer Research (2014)

Abstract Quiescent cancer cells are resistant to cytotoxic agents which target only proliferating cancer cells. Time-lapse imaging showed that Salmonella typhimurium A1-R (A1-R) encircled and invaded quiescent cancer cells in monolayer culture, thereby eliminating them. Moreover A1-R infected and killed quiescent fluorescent ubiquitination-based cell cycle indicator (FUCCI)-expressing cancer cells in tumor spheres. In contrast, cytotoxic agents did not kill quiescent cancer cells in tumor spheres. A1-R infection of FUCCI-expressing subcutaneous tumors growing in nude mice resulted in killing quiescent cancer cells resistant to cytotoxic agents. Furthermore, the combination of A1-R and cisplatin reduced tumor size compared with A1-R monotherapy or cisplatin alone. This study demonstrates that A1-R can kill quiescent cancer cells and also sensitize them to conventional chemotherapy. These results suggest a new therapeutic paradigm potentially more effective than current therapeutics which are ineffective against quiescent cancer cells. Citation Format: Shuya Yano, Yong Zhang, Ming Zhao, Yukihiko Hiroshima, Shinji Miwa, Fuminari Uehara, Hiroyuki Kishimoto, Hiroshi Tazawa, Toshiyoshi Fujiwara, Robert M. Hoffman. Salmonella typhimurium A1-R induces quiescent FUCCI-expressing cancer cells to cycle and become chemosensitive. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 711. doi:10.1158/1538-7445.AM2014-711
Prospective monitoring and quantitation of residual blasts in childhood acute lymphoblastic leukemia by polymerase chain reaction study of delta and gamma T-cell receptor genes
Authors: H Cave, C Guidal, P Rohrlich, MH Delfau, A Broyart, B Lescoeur, C Rahimy, O Fenneteau, N Monplaisir, L d'Auriol
Journal: Blood (1994)