tbuckley/cabot-search huggingface dataset:
Degradation and intracellular accumulation of a residualizing hyaluronan derivative by liver endothelial cells
Authors: Carl T. McGary, Judith Yannariello-Brown, Dennis W. Kim, Tami C. Stinson, Paul H. Weige
Journal: Hepatology (1993)

The release and intracellular accumulation of 125I-hyaluronan degradation products was studied in cultured liver endothelial cells with hyaluronan oligosaccharides (relative molecular mass = approximately 44,000) uniquely modified and radiolabeled at the terminal reducing sugar. Two methods were combined to measure 125I-hyaluronan degradation by liver endothelial cells. (a) Cetylpyridinium chloride precipitation of hyaluronan oligosaccharides was used as a rapid, convenient assay to monitor the appearance of hyaluronan degradation products. Hyaluronan oligosaccharides less than 54 to 60 monosaccharides in length were not precipitated with cetylpyridinium chloride and thus were assessed as degraded. (b) Gel filtration chromatography was used to estimate the size range of oligosaccharides produced by liver endothelial cells. After internalization of 125I-hyaluronan, liver endothelial cells released radioactive degradation products into the culture media after a lag period of 2.5 to 3.0 hr. The intracellular accumulation of degraded 125I-hyaluronan was linear for at least 2 hr even though no degradation products were released. The long lag before release of degraded 125I-hyaluronan is likely caused by the modified chemical structure at the reducing end of the hyaluronan derivative; the derivative acts like a residualizing label. After this lag the release of degraded 125I-hyaluronan proceeded linearly for up to 12 hr. The extracellular 125I-hyaluronan degradation products eluted with a distribution coefficient of 1.3 on a gel filtration column. The major intracellular 125I-labeled degradation product showed the same retardation (distribution coefficient = 1.3). This retention may be caused by the hydrophobic aromatic and alkyl modifications to the former reducing sugar, also characteristics of a residualizing label. In addition, at least two larger minor intermediates were observed intracellularly. The rate of intracellular 125I-hyaluronan degradation was dependent on hyaluronan concentration and reached a maximal rate (159 molecules/cell/sec) at 2 x 10(-7) mol/L. This was about half the maximal rate of endocytosis (285 molecules/cell/sec) at a hyaluronan concentration of 1.3 x 10(-7) mol/L. The apparent ligand concentration that gives half-maximal responses for endocytosis and intracellular degradation was 0.6 x 10(-7) and 1.0 x 10(-7) mol/L, respectively.
Doctors' pay is one reason for NHS debt
Authors: Zosia Kmietowicz
Journal: BMJ (2007)

A report by the government's spending watchdog has blamed poor financial management; lack of input from clinicians on financial matters; and underestimates by the Department of Health of the cost of doctors' recent pay awards for the NHS's current financial troubles.

For its report, the House of Commons' Public Accounts Committee took evidence from the Department of Health on the cause of financial deficits in NHS organisations, their impact, and what steps were being taken to recover deficits.

Errors in costing GPs' and consultants' contracts meant that, in 2005-6, individual NHS bodies were required to fund a £560m (€830m; $1.1bn) shortfall in resources to meet agreed pay awards.

Edward Leigh MP, chairman of the committee, said that there was no one factor that had led to the cumulative deficit for all NHS trusts at the end of …
Demeclocycline-Induced Diabetes Insipidus
Authors: Alberto Hayek
Journal: JAMA (1974)

Two patients developed diabetes insipidus during or following the administration of demeclocycline hydrochloride. The data suggest that this antibiotic interferes with the action of antidiuretic hormone in the renal tubule. Both patients recovered their urinary concentrating ability one week after therapy was discontinued. (<i>JAMA</i>229:676-677, 1974)
Breathing and relaxation technique cut asthma symptoms by one third
Authors: Susan Mayor
Journal: BMJ (2007)

A sequence of breathing and relaxation exercises in patients with mild asthma reduced asthma symptoms by one third, according to results from a randomised controlled trial ( Thorax 2007 Jun 15 doi: 10.1136/thx.2006.076430).

Eighty five people with mild or moderate asthma were recruited from the asthma register of a semirural general practice in the United Kingdom. They were randomly assigned to a breathing and relaxation technique known as the Papworth method, in addition to their usual drug, or simply to remain on their usual drug therapy.

Patients assigned to the breathing method attended five one hour training sessions with a respiratory physiotherapist. It combines diaphragmatic breathing, breathing through the nose, and relaxation exercises to reduce anxiety and symptoms arising from hypocapnia.

Patients using the Papworth …
COMBINATION SEDAN AND AMBULANCE
Authors: John B. Potts
Journal: JAMA (1923)

In many communities where the demand for a regular ambulance is not great, a sedan that has the doors coming together at the center can be converted into an ambulance with very little difficulty. The change is made by removing the center post, which is held at the bottom by metal dowels, and at the top by a wing nut. After the post is removed, the larger half of the front seat and the auxiliary seat is taken out, and a board is laid on the floor to make a level surface, on which a carpet is laid. This is done quickly, and requires little or no skill. In a sedan thus converted, the space between the front and rear of the car is sufficiently wide to hold a Bomgardner cot. The foregoing can be furnished by Mr. Pfeiffer of the Pfeiffer Top and Body Corporation, Omaha.
Grandparental investment facilitates harmonization of work and family in employed parents: A lifespan psychological perspective
Authors: Christiane A. Hoppmann, Petra L. Klumb
Journal: Behavioral and Brain Sciences (2010)

Abstract The target article emphasizes the need to identify psychological mechanisms underlying grandparental investment, particularly in low-risk family contexts. We extend this approach by addressing the changing demands of balancing work and family in low-risk families. Taking a lifespan psychological perspective, we identify additional motivators and potential benefits of grandparental investment for grandparents themselves and for subsequent generations.
Paroxysmal Nocturnal Hemoglobinuria, a Clue Disease
Authors: Carl F. Hinz
Journal: New England Journal of Medicine (1973)

Paroxysmal nocturnal hemoglobinuria has long held a special place among hematologists as a "clue disease" — one of the relatively uncommon disorders that promises insights not only into its own mechanisms but also into biologic reactions of a more general nature. As an example, it has been a major model for exploration of the properdin (or alternate) pathway of complement action, which is responsible for hemolysis of the patient's red cells in acidified normal serum, a hallmark of this disease.The investigation of mechanisms in nocturnal hemoglobinuria spans nearly 40 years. In 1937 Hale Ham, working at the Thorndike Laboratory, . . .
The Changing Managed Care–Public Health Interface
Authors: William L. Roper
Journal: JAMA (1998)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Controlled release of rhBMP-2 loaded poly(dl-lactic-co-glycolic acid)/calcium phosphate cement composites in vivo
Authors: P.Q. Ruhé, O.C. Boerman, F.G.M. Russel, P.H.M. Spauwen, A.G. Mikos, J.A. Jansen
Journal: Journal of Controlled Release (2005)
Occupational asthma due to ceftazidime
Authors: SC Stenton, JH Dennis, DJ Hendrick
Journal: European Respiratory Journal (1995)

A worker employed in the manufacture of the new third generation cephalosporin antibiotic, ceftazidime, developed asthmatic symptoms, and a series of inhalation challenge tests was undertaken to investigate the problem. The inhalation of increasing daily doses of ceftazidime up to 3.2 mg, using a double-blind challenge protocol gave rise to symptoms, late asthmatic reactions, and increases in airway responsiveness to methacholine. A repeat challenge with the 3.2 mg dose additionally gave rise to a clear immediate reaction. Ceftazidime was, thus, shown to be capable of inducing occupational asthma.
No mana for the shaman
Authors: I. Robertson
Journal: BMJ (1995)

A few months ago two members of the rock band KLF burned a million pounds in the fireplace of a bothy on a remote Scottish island. They wanted to be artists, and this was their artistic statement. Then one of their friends tried to hawk a case full of the ashes round some London galleries, “complete with full documentation.” A futile act of vandalism was suddenly transformed into quite a useful social act by showing up a section of the modern art world for the charade that it is.

“Anyone can't just …
Abstract 707: Genetically Modified Mesenchymal stem cells (MSCs) Overexpressing Chemokine Receptor CCR1 Improves Cellular Engraftment and Tissue Repair of Ischemic myocardium
Authors: Jing Huang, Jian Guo, Aiguo Ni, Ajun Deb, Lunan Zhang, Richard Prattre, Victor J Dzau
Journal: Circulation (2007)

Bone marrow-derived mesenchymal stem cells (BM-MSCs) may promote structural and functional repair in the myocardium following acute myocardial infarction (MI). However, the cytotactic factors/receptors responsible for MSC engraftment to the injured myocardial tissue have not been fully elucidated. Chemokines, important agents controlling cell locomotion and trafficking, are upregulated in the heart following MI and have been implicated in regulating engraftment and homing of MSCs to infarcted tissue. We hypothesized that over expression of certain chemokine receptors in MSCs will augment cell engraftment of injected MSCs in infarcted tissue. Our previous experiments indicated that CCL7(/MCP3) and SDF-1 expression was upregulated in infarcted myocardium. However, the level of expression of receptors corresponding to these chemokines in MSCs is low. In this study, we retrovirally transduced murine BM-MSCs with CCR1 or CXCR4 (receptors for CCL7 and SDF-1, respectively) genes under the control of the constitu...
Comparing cannabis with tobacco: Services are needed for acute and chronic effects of cannabis
Authors: R. Robertson
Journal: BMJ (2003)

EDITOR—Henry et al draw attention to the damaging effects of cannabis and the potential problems likely to emerge from its increasing use.1

There are many aspects to this debate, not least the inevitability of the progress to further use, although other countries have shown this to peak in young people and to deteriorate subsequently. It is also, like most addiction problems, …
Stromal CD8+ T-cell Density—A Promising Supplement to TNM Staging in Non–Small Cell Lung Cancer
Authors: Tom Donnem, Sigurd M. Hald, Erna-Elise Paulsen, Elin Richardsen, Samer Al-Saad, Thomas K. Kilvaer, Odd Terje Brustugun, Aslaug Helland, Marius Lund-Iversen, Mette Poehl, Karen Ege Olsen, Henrik J. Ditzel, Olfred Hansen, Khalid Al-Shibli, Yury Kiselev, Torkjel M. Sandanger, Sigve Andersen, Francesco Pezzella, Roy M. Bremnes, Lill-Tove Busund
Journal: Clinical Cancer Research (2015)

Immunoscore is a prognostic tool defined to quantify in situ immune cell infiltrates, which appears to be superior to the tumor-node-metastasis (TNM) classification in colorectal cancer. In non-small cell lung cancer (NSCLC), no immunoscore has been established, but in situ tumor immunology is recognized as highly important. We have previously evaluated the prognostic impact of several immunological markers in NSCLC, yielding the density of stromal CD8(+) tumor-infiltrating lymphocytes (TIL) as the most promising candidate. Hence, we validate the impact of stromal CD8(+) TIL density as an immunoscore in NSCLC.The prognostic impact of stromal CD8(+) TILs was evaluated in four different cohorts from Norway and Denmark consisting of 797 stage I-IIIA NSCLC patients. The Tromso cohort (n = 155) was used as training set, and the results were further validated in the cohorts from Bodo (n = 169), Oslo (n = 295), and Denmark (n = 178). Tissue microarrays and clinical routine CD8 staining were used for all cohorts.Stromal CD8(+) TIL density was an independent prognostic factor in the total material (n = 797) regardless of the endpoint: disease-free survival (P < 0.001), disease-specific survival (P < 0.001), or overall survival (P < 0.001). Subgroup analyses revealed significant prognostic impact of stromal CD8(+) TIL density within each pathologic stage (pStage). In multivariate analysis, stromal CD8(+) TIL density and pStage were independent prognostic variables.Stromal CD8(+) TIL density has independent prognostic impact in resected NSCLC, adds prognostic impact within each pStage, and is a good candidate marker for establishing a TNM-Immunoscore.
THE OPEN-AIR TREATMENT OF PNEUMONIA.
Authors: W. P. NORTHRUP
Journal: JAMA (1906)

For more than eleven years I have been treating pneumonia patients in cool fresh air, gradually placing their beds nearer and nearer the open window. Last winter I put them out on the roof. It is not always practicable to make use of a roof, but what I have to say about the beneficial effects of open-air treatment is founded mainly on last year's experience at the Presbyterian Hospital, New York City. It comprises experience in all months of the winter. Our systematic practice was to put all pneumonia patients during the day, for six hours, on the roof, in the open air, in all weather in which harsh high winds, rain and snow did not prohibit. Indeed, the patients were not always brought in for little sprinkling rains or trivial snowfalls, and many times were out when high snow banks formed a corral about the space in which the
Using Exhaled Nitric Oxide and Serum Periostin as a Composite Marker to Identify Severe/Steroid-Insensitive Asthma
Authors: Tadao Nagasaki, Hisako Matsumoto, Yoshihiro Kanemitsu, Kenji Izuhara, Yuji Tohda, Takahiko Horiguchi, Hideo Kita, Keisuke Tomii, Masaki Fujimura, Akihito Yokoyama, Yasutaka Nakano, Soichiro Hozawa, Isao Ito, Tsuyoshi Oguma, Yumi Izuhara, Tomoko Tajiri, Toshiyuki Iwata, Junya Ono, Shoichiro Ohta, Tetsuji Yokoyama, Akio Niimi, Michiaki Mishima
Journal: American Journal of Respiratory and Critical Care Medicine (2014)
Hospital Readmissions Following Surgery
Authors: Lucian L. Leape
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Bladder Evacuation in Paraplegic Dogs by Direct Electric Stimulation
Authors: Adrian Kantrowitz, Martin Schamaun
Journal: JAMA (1964)

EARLY RESULTS of direct electric stimulation of the urinary bladder in paraplegic dogs have been previously reported.<sup>1,2</sup>Two stainlesssteel wire electrodes were implanted in the smooth muscle itself for application of a train of biphasic square waves generated by a stimulator. Parameters of the most effective stimulus were one to six milliseconds' duration and 15 to 35 cycles per second frequency. The best response was obtained with 4 msec and 20 cps (Fig 1). This corresponds rather well with the findings of Burghele and associates,<sup>3</sup>as well as with those of Bradley et al.<sup>4</sup> In experiments on 12 normal dogs and 17 animals whose lumbar spinal cords had been transected, a catheter was inserted through a small cystotomy during surgery. Intravesical pressure, registered with a strain-gauge and recorder, exceeded 40 cm of water with 2.5- to 10-v stimuli in all but two dogs. In most of the 17 paraplegic
Is there more to visual attention than meets the eye?
Authors: Cyril Latimer
Journal: Behavioral and Brain Sciences (1999)

Models of saccade generation and visual selective attention must explain how and why particular targets are selected. Findlay &amp; Walker do an excellent job of explaining the how of visual selection, but not the why. For a salience map to be more than a description of the relative importance of potential targets, there must be some account of the learning and inheritance that fashion its peaks and troughs. Point of gaze is not necessarily region of attention, and it may be premature to downgrade the role of covert attention in models of visual selection.
Lower Rates of Heart Failure and Death in Acute Coronary Syndromes
Authors: Lydia A. Bazzano
Journal: JAMA (2007)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Role of the H-2 complex in induction of T helper cells in vivo. I. Antigen-specific selection of donor T cells to sheep erythrocytes in irradiated mice dependent upon sharing of H-2 determinants between donor and host.
Authors: J Sprent
Journal: The Journal of Experimental Medicine (1978)

When purified CBA lymph node T cells were mixed with sheep erythrocytes (SRC) and filtered from blood to lymph through irradiated syngeneic mice for 1-2 days, the donor cells lost their capacity to stimulate anti-SRC responses by CBA B cells; the response to a third-party antigen (horse erythrocytes) was unaffected and active suppression was not involved. This process of specific negative selection to SRC also occurred when semiallogeneic mice were used as filtration hosts. By contrast, when allogeneic hosts were used the helper function of the donor cells was not reduced; this applied to both primed and unprimed T cells. Studied with congeneic resistant strains indicated that negative selection to SRC occurred only when the donor and host shared H-2 determinants. Studies with T cells depleted of alloreactive lymphocytes showed that negative selection to SRC in irradiated F1 hybrid mice was followed by a stage of positive selection where the donor cells gave greatly increased responses to the injected antigen. Positive selection did not occur in H-2-different mice, however, and the helper function of the donor cells remained unchanged. By these parameters it was concluded that homozygous T helper cells have no detectable capacity to recognize antigen in an H-2-different environment.
Endotoxemia contributes to the immune paralysis in patients with cirrhosis
Authors: Chun-Yen Lin, I-Fan Tsai, Yu-Pin Ho, Ching-Tai Huang, Yung-Chang Lin, Chun-Jung Lin, Shu-Chin Tseng, Wei-Pin Lin, Wei-Ting Chen, I-Shyan Sheen
Journal: Journal of Hepatology (2007)
'Saw-Tooth' Pattern on a Flow-Volume Loop Following Neck Surgery
Authors: James M. Kohan
Journal: JAMA (1984)

<h3>To the Editor.—</h3> Sanders et al<sup>1</sup>reported the "saw-tooth" sign of the flow-volume loop as indicating a likelihood of significant sleep-associated upper airway obstruction (UAO). They emphasized well-defined, regular oscillations occurring at constant intervals on forced expiratory and/or inspiratory flow-volume tracings as a phenomenon attributable to structural or functional abnormality in the upper airway. In 21 subjects studied, 13 had sleep apnea hypersomnolence syndrome associated with UAO (SAHS-UAO). Eleven of the 13 patients had "saw-toothing" on the flow-volume loop, which correlated with fluttering of tissue visualized in the upper airway via bronchoscopy. Specificity of the saw-toothing was 100% in their study group, but the authors cautioned in their conclusion that this pattern may simply represent an interplay of physical forces within the upper airway that may or may not have clinical manifestations. We recently saw a patient with a saw-tooth pattern whose clinical status and follow-up study findings question