tbuckley/cabot-search huggingface dataset:
829. Preferential Transfection of Tissue Macrophages Following Intravenous Administration of sec-R Targeted hCFTR DNA Enhances Survival of CF Mice Inoculated with Pseudomonas aeruginosa Agar Beads
Authors: Assem G. Ziady, James Poleman, Christiaan van Heeckeren, Anna van Heeckeren, Pamela B. Davis
Journal: Molecular Therapy (2004)

Previously, we have shown that modification of Poly K compacted DNA complexes with a peptide ligand (C105Y) that binds the serpin enzyme complex receptor (sec-R) targets them to lung, liver and spleen after intravenous (IV) administration and to airway epithelial cells after intranasal (IN) administration in CF mice. Predominantly, tissue macrophages were transfected following IV dosing. Since both epithelial cells and macrophages express CFTR, we took advantage of these predilection to test whether CFTR delivery to macrophages or to epithelial cells protected against the consequences of Pseudomonas aeruginosa infection. We tested the effect of different routes of administration of sec-R targeted hCFTR, IN versus IV, on alleviating the consequence of the inoculation of CF mice with Pseudomonas aeruginosa laden agar beads. This method of infection induces inflammation in CF mice that models advanced disease in CF patients. Three groups of S489X/FABP-hCFTR mice (n=14 per group) housed in sterile micro-isolator cages were studied. Group 1 received a 50 μl bolus of a 1.0 M NaCl solution containing 10 μg of sec-R targeted compacted hCFTR plasmid (codes for human CFTR driven by the elongation factor 1 promoter) via the tail vein injection and 50 μl 1.0 M NaCl alone IN. Group 2 received 50 μl sec-R targeted hCFTR IN and 50 μl 1.0 M NaCl alone IV. Control group animals received 50 μl 1.0 M NaCl IV and IN. One day following dosing the mice were inoculated with 5 × 104 cfu P. aeruginosa embedded in agar beads, and their weights recoded. We monitored the mice for 10 days following infection. Ten of fourteen mice that received IV administration of sec-R targeted hCFTR survived the course of the experiment, while 6/14 survived for the IN dosed group and 3/14 survived for the control group (p=0.03, ANOVA). Weight loss that occurs following infection was reversed one day earlier on day 4 for groups 1 and 2 versus day 5 for control animals. These results were reproduced in 2 other experiments. In a fourth experiment, analysis of bronchoalveolar lavage (BAL) from animals similarly dosed and infected, but sacrificed on day 3 following infection revealed no significant differences in cell counts or production of TNF-α, IL-1β, IL-6, or mip-2/KC (mouse IL-8 homologs). Based on previous observations, these data suggest that delivery of hCFTR to tissue macrophages rather than airway epithelial cells allows for better protection against and/or recovery from infection in CF mice. Subtle decreases in cytokine production may be sufficient for this, although other event involved in infection and inflammation not assayed for by us may have been impacted more significantly by hCFTR gene transfer to tissue macrophages.
739. Lentiviruses as Gene Transfer Vehicles for Cancer Gene Therapy
Authors: Riikka Pellinen, Tanja Hakkarainen, Tiina Pasanen, Kirsi Tulimaki, Anna Ketola, Anni Tenhunen, Pekka Timonen, Jarmo Wahlfors
Journal: Molecular Therapy (2004)

Lentiviral vectors are very efficient gene transfer vehicles for most cell types, regardless of the species or tissue of origin. Several reports suggest that these vectors are efficient for most human tumor cells, but their utility in cancer gene therapy has not been examined systematically. To study this, VSV-G pseudotyped HIV-1 vector and E1/E3 deleted adenoviral vector (serotype 5) were compared for their capability to transduce 42 different human cancer cell lines. In most cases MOI 3 yielded 50-90 % transduction efficiency with both vectors. In vivo studies with nude mouse s.c. tumor model (A549 lung cancer cells) revealed that lentiviruses were more efficient vehicles than adenoviruses when same amount of virus was used.
Mutations in the gene encoding neutrophil elastase in congenital and cyclic neutropenia
Authors: David C. Dale, Richard E. Person, Audrey Anna Bolyard, Andrew G. Aprikyan, Cindy Bos, Mary Ann Bonilla, Laurence A. Boxer, George Kannourakis, Cornelia Zeidler, Karl Welte, Kathleen F. Benson, Marshall Horwitz
Journal: Blood (2000)
THE INFLUENCE OF AGE, SEX, HYPOPHYSECTOMY AND VARIOUS HORMONES ON THE COMPOSITION OF THE SKIN SURFACE LIPIDS OF THE RAT.
Authors: T. NIKKARI, M. VALAVAARA
Journal: British Journal of Dermatology (1970)

SUMMARY.— Composition of the skin surface lipids of rats aged 3–14 weeks was analysed with thin‐layer and gas‐liquid chromatography. It was found that hypophysectomy of female rats aged 3 weeks was followed by distinct changes in the relative amounts of both the unhydrolysed lipid fractions and the different fatty acids. The changes in composition brought about by testosterone treatment in the hypophysectomized animals and by age in the intact male rats were in many respects parallel with each other and opposite to the changes caused by hypophysectomy. The pre‐hypophysectomy composition was not, however, completely restored by testosterone or by simultaneous treatment with both testosterone and oestradiol. Oestradiol had no clearly specific effect on the composition, but growth hormone given to the hypophysectomized animals appeared to give partial protection against the changes in composition produced by hypophysectomy. The ratio of palmitic to stearic acid was found to be of value as an indicator of the activity of the sebaceous glands. There was a good positive correlation between this ratio and the production rate of sebum.
New Combination Therapy for Advanced Breast Cancer
Authors: Bernard A. Schwetz
Journal: JAMA (2001)

The FDA has approved capecitabine (Xeloda, Hoffman LaRoche Inc, Nutley, NJ) combined with docetaxel (Taxotere, Aventis Pharmaceuticals, Parsippany, NJ) for treating patients with metastatic breast cancer after failure of prior anthracycline-containing chemotherapy.
Abstract 132: Compliance Changes in the Aorta Following Chest Compressions in a Standard and High Impulse Model
Authors: Matthew Sundermann, David Salcido, James Menegazzi
Journal: Circulation (2014)

Introduction: In cardiopulmonary resuscitation (CPR), chest compressions eject blood from the aorta and into the circulation. Following return of spontaneous circulation (ROSC), the circulation generated by the heart may be hemodynamically compromised, notably in its aortic compliance, or the aorta’s ability to resist blood pressure changes in response to blood volume changes. We investigated the relationship between CPR force delivery and relative change in aortic compliance in standard and high impulse chest compression models. Hypothesis: We hypothesized that average compliance would increase following chest compressions and that high impulse CPR would yield larger compliance changes than compliance changes in standard CPR. Methods: Central aortic pressure (CAP) waveforms were retrospectively analyzed via Millar catheters in female swine separated into standard CPR (n=4) and high impulse CPR (n=5). Ventricular fibrillation was induced and left untreated for 8 minutes, followed by 5 minutes of CPR and drugs. Rescue shocks were then delivered. Compliance was defined as stroke volume divided by the pressure difference between the dicrotic notch and end diastole. Stroke volume was estimated by using a previously validated model, which defined stroke volume as the area beneath the systolic portion of the CAP waveform multiplied by both a calibration constant and a ratio of time in diastole over time in systole. Compliances were calculated from CAP waveforms in ten consecutive beats before and after CPR with custom software built using MATLAB. A 2-way ANOVA was performed on average compliance across ten beats before and after chest compressions in both standard and high impulse CPR. Results: The average compliance (mL/mmHg) at baseline or pre-CPR (203), was significantly different from the average compliance post-CPR (335) (p=.03). The compliance change in standard CPR (151) did not significantly differ from compliance change in high impulse CPR (116) (p=.27). Conclusion: We observed a significant compliance increase in beats post-CPR compared to beats pre-CPR in both standard and high impulse groups. A significant difference in compliance change was not found when comparing standard vs. high impulse CPR.
Abstract 17545: Different Human Mutations in the Myosin Binding Protein C3 (MYBPC3) Produce Specific Cardiac Phenotypes in the Zebrafish
Authors: Sahar I Da’as, Joseph Yu, Jonathan T Butcher, Navaneethakrishnan Krishnamoorthy, Jassim Al Suwaidi Al Suwaidi, Heba Kassem, Kholoud N Al Shafai, Mohammed A Al-Hashemi, Lama Shuayb, Thomas Brand, Magdi H Yacoub
Journal: Circulation (2014)

Mutations in the gene encoding myosin binding protein C3 (MYBPC3) are one of the commonest causes of Hypertrophic cardiomyopathy and can produce varying phenotypes. The exact disease mechanisms responsible remain unknown. Zebrafish model offers unique opportunities to study human cardiovascular disease mechanisms in vivo. We have modeled different human MYBPC3 mutations in zebrafish embryos. Morpholinos targeting zebrafish mybpc3 were injected to model four disease causing missense mutations of domain C1. Mutation1 (Arg177His), Mutation 2 (Ala216Thr), Mutation 3 (Glu258Lys) that were identified in Egypt for the first time by Kassem et al [1] and Mutation 4 (Ser217Gly) that was recently identified in Qatar. The morpholino targets Mutation 1, 2 and 4 at exon 5 and Mutation 3 at exon 6 splice donor site in zebrafish embryos, in order to precisely recapitulate the human mutations. Different MYBPC3 human mutations produced specific cardiac phenotypes in zebrafish embryos. These morphant embryos (3 days old) di...
Abstract 15590: Effect of Home-Based Follow up Intervention on Readmissions and Mortality in Heart Failure Patients - A Meta-analysis of Randomized Controlled Trials
Authors: Aaqib H Malik, Yasir Akram, Suchith Shetty, Senada S Malik, Faizan A Syed, Haq Nawaz
Journal: Circulation (2014)

Introduction: Congestive heart failure (CHF) is associated with significant morbidity, recurrent re-admissions, poor overall prognosis and increased mortality. Our aim was to determine whether a home-based intervention (HBI) improves re-admissions or mortality outcomes compared to usual care for heart failure patients. Methods: Medline Indexed and Non-indexed, Embase, and PsychINFO were searched for randomized controlled trials (RCTs).All RCTs that examined the impact of HBI in CHF patients. The studies with sample size <50 and those that used tele-medicine monitoring were excluded. Two independent investigators assessed the studies against an a priori inclusion criteria and disagreements were resolved by mutual discussion. Results: We used reported event rates for all studies to compute cumulative odds ratio and p-value for re-admission rate and mortality. Pre-specified subgroup analysis was performed to assess the difference in readmission rate and mortality according to the length of follow-up. Summary...
Abstract 16821: Reductions in Vascular Function Correlate with Age in a Novel Non-Human Primate Model for Assessment of Vascular Health
Authors: Richard L Schroeder, Andrew H Smith, David A Beebe, Sharon A Sokolowski, Chunli Huang, Delvin R Knight
Journal: Circulation (2011)

Background: Non human primates develop cardiovascular disease over decades. Whether the progression of vascular dysfunction with age is like that observed in humans is unclear. We hypothesize that, like humans, monkeys show age-related reductions in flow-mediated dilation (FMD, an index of endothelium-dependent vascular reactivity) and increases in pulse-wave velocity (PWV, an index of aortic stiffness). We also explored a potential relationship between the age-related vascular effects and circulating biomarkers of endothelial health, endothelial microparticles (EMP) and endothelial progenitor cells (EPC). Methods and Results: In M. fascicularis monkeys (N=21, 14 males and 7 females, 6 to 25 years of age), blood was sampled for EPC and EMP that were measured using flow cytometry. Monkeys were then sedated (midazolam + torbugesic) and baseline femoral artery diameter (4mm segment) was measured by ultrasonography. FMD was calculated as the peak %change from baseline diameter following a 5-minute cessation of blood flow below the knee. PWV was calculated using a modified SphygmoCor program and pulse waves from the radial and femoral arteries. FMD and PWV ranged from 6 to 33% and from 3 to 7 m/s, respectively, and multiple samplings showed high reproducibility up to 4 months (inter-assay variation &lt;5%). FMD negatively (r=-0.94, p&lt;0.0001), while PWV positively (r=0.60, p&lt;0.002) correlated with age in these monkeys. Numbers of EPC (CD45- CD31+ CD34+ VEGFR2+) and EMP [CD45- CD42a- CD54(ICAM-1)+, CD45- CD42a- CD105(endoglin)+, and CD45- CD42a- CD144(VE-cadherin)+] were highly variable between individuals. Neither the EPC nor any of the EMPs significantly correlated with the age or age-related changes in FMD or PWV. Significance = p&lt;0.05. Conclusion: For the first time in non-human primates, we established two non-invasive, clinically-relevant assessments of vascular function, FMD and PWV. Like humans, monkeys show age-related reductions in endothelium-dependent vascular reactivity and increases in aortic stiffness. The circulating numbers of EMP and EPC, independent of the chronic, age-related changes in FMD and PWV, may better serve as biomarkers of acute changes in vascular health associated with disease or therapeutic interventions.
Quality of Life Improvements after Acceptance and Commitment Therapy in Nonresponders to Cognitive Behavioral Therapy for Primary Insomnia
Authors: Elisabeth Hertenstein, Nicola Thiel, Marianne Lüking, Anne Katrin Külz, Elisabeth Schramm, Chiara Baglioni, Kai Spiegelhalder, Dieter Riemann, Christoph Nissen
Journal: Psychotherapy and Psychosomatics (2014)

years; no relevant comorbidities). All participants had extensive experience with CBT-I according to evidence-based guidelines ( ≥ 6 sessions; period of time between CBT-I and inclusion in the present study: 2.2 ± 3.4 years) and continued suffering from clini-cally relevant insomnia (sleep onset latency or wake time after sleep onset: ≥30 min, ≥3 days/week). Seven participants were free of psychoactive medication; 3 had been on stable medication for ≥ 3 years. All participants were diagnosed in our sleep laboratory based on a clinical interview and standard physical examinations and provided written informed consent prior to the study. The study has been approved by the local ethics committee and has been registered in the German Clinical Trials Register prior to the enrollment of the first participant. The open pilot study comprised 2 baseline measurements [6 weeks before (T
Abstract 1945: Low Endothelial Shear Stress (ESS)-Mediated Elastolytic Activity Promotes the Formation of High Risk Atherosclerotic Plaques in the Coronary Arteries
Authors: Yiannis S Chatzizisis, Aaron B Baker, Galina Sukhova, Roy Beigel, Michael Jonas, Ahmet U Coskun, Benjamin Stone, Charles Maynard, Ross Gerrity, Guo Ping Shi, Peter Libby, Peter H Stone, Charles L Feldman, Elazer R Edelman
Journal: Circulation (2008)
Abstract 3500: NCI's Provocative Questions Initiative
Authors: Michelle A. Berny-Lang, Emily J. Greenspan
Journal: Cancer Research (2014)

Abstract In 2011, the NCI Director, Dr. Harold Varmus, created the Provocative Questions (PQ) initiative to encourage imaginative, bold approaches designed to tackle perplexing and previously neglected areas of cancer research. Based upon workshops and discussions with the extramural cancer research community and NCI program directors, a set of 24 questions and accompanying requests for applications (RFAs) were developed, addressing diverse topics in cancer risk and prevention, tumor development, and cancer detection, diagnosis, and treatment. In order to stimulate innovative hypotheses and approaches, the “power of the ideas” was heavily weighted with a de-emphasis on preliminary data. In the first year of the initiative, the NCI awarded 56 unique proposals, totaling $21.5 million of research support via the R01 and R21 mechanisms. Building upon the 2011 RFAs and adjusting to changes in the research landscape, 24 revised questions were developed for the 2012 RFAs with a commitment of up to $30 million in support. The NCI released the 2013 RFAs in September 2013 with a revised set of 20 questions, including new questions related to clinical effectiveness. Several PQ-supported research projects funded in the first year of the initiative will be highlighted in order to demonstrate the breadth of topics and high level of innovation supported by the initiative. Projects will be selected from the four main PQ thematic areas to provide a sense of the many diverse approaches being used to answer the selected PQ. In addition, trends and criteria from awarded proposals will be presented to provide insight to potential applicants regarding the outcomes of the program to date. Through engagement of the cancer research community, the PQ initiative seeks to continually leverage scientific advances to address currently unsolved, understudied questions in cancer, which may influence the future directions of NCI-sponsored research. Citation Format: Michelle A. Berny-Lang, Emily J. Greenspan. NCI's Provocative Questions Initiative. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3500. doi:10.1158/1538-7445.AM2014-3500
Exercise performance in patients with end-stage heart failure after implantation of a left ventricular assist device and after heart transplantation
Authors: Nicolaas de Jonge, Hans Kirkels, Jaap R. Lahpor, Corinne Klöpping, Erik J. Hulzebos, Aart Brutel de la Rivière, Etienne O. Robles de Medina
Journal: Journal of the American College of Cardiology (2001)
Age as a Risk Factor for Stroke in Atrial Fibrillation Patients
Authors: Ricarda Marinigh, Gregory Y.H. Lip, Nicola Fiotti, Carlo Giansante, Deirdre A. Lane
Journal: Journal of the American College of Cardiology (2010)

The prevalence of atrial fibrillation (AF) is related to age and is projected to rise exponentially as the population ages and the prevalence of cardiovascular risk factors increases. The risk of ischemic stroke is significantly increased in AF patients, and there is evidence of a graded increased risk of stroke associated with advancing age. Oral anticoagulation (OAC) is far more effective than antiplatelet agents at reducing stroke risk in patients with AF. Therefore, increasing numbers of elderly patients are candidates for, and could benefit from, the use of anticoagulants. However, elderly people with AF are less likely to receive OAC therapy. This is mainly due to concerns about a higher risk of OAC-associated hemorrhage in the elderly population. Until recently, older patients were under-represented in randomized controlled trials of OAC versus placebo or antiplatelet therapy, and therefore the evidence base for the value of OAC in the elderly population was not known. However, analyses of the available trial data indicate that the expected net clinical benefit of warfarin therapy is highest among patients with the highest untreated risk for stroke, which includes the oldest age category. An important caveat with warfarin treatment is maintenance of a therapeutic international normalized ratio, regardless of the age of the patient, where time in therapeutic range should be ≥65%. Therefore, age alone should not prevent prescription of OAC in elderly patients, given an appropriate stroke and bleeding risk stratification.
Serum interleukin-10 levels as a prognostic factor in advanced non-small cell lung cancer patients
Authors: Marina Petrovic, Ivan Cekerevac, Zorica Lazic, Vladimir Zdravkovic
Journal: European Respiratory Journal (2013)

Introduction :   IL-10 serum levels were measured before chemotherapy, on completion of therapy, and at follow-up by means of a commercially available enzyme-linked immunoassay.  Methods  :  Sixty consecutive patients,stage III or IV NSCLC undergoing conventional platinum-based regimens. Results  :  Elevated levels of serum IL-10 were found in cancer patients with respect to healthy control subjects (15.2 vs 7.2 pg/mL; p  Conclusions : In conclusion, this study shows that the measurement of pretreatment IL-10 serum levels is of independent prognostic utility in patients with NSCLC and may be useful for detection of disease progression.
Tırnak pigmentasyonlarında tanı ve yaklaşım
Authors: Ralph Peter Braun, Robert Baran, Frederique Anne Le Gal, Stephane Dalle, Sandra Ronger, Roberta Pandolfi, Olivier Gaide, Lars Einar French, Paul Laugier, Jean Hilaire Saurat, Ashfaq Ahmed Marghoob, veLuc Thomas
Journal: Journal of the American Academy of Dermatology (2007)
Nitric Oxide Modulation of Neutrophil-Endothelium Interaction: Difference Between Arterial and Venous Coronary Bypass Grafts 11This work was supported in part by a grant from the Italian Ministry of University and Scientific Research, Rome, Italy.
Authors: Massimo Chello, Pasquale Mastroroberto, Francesco Perticone, Vittoria Celi, Alfredo Colonna
Journal: Journal of the American College of Cardiology (1998)
Telephone Psychotherapy and Care Management for Depression—Reply
Authors: Gregory Simon, Evette Ludman, Steve Tutty, Belinda Operskalski, Michael Von Korff
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Gestational Diabetes Mellitus
Authors: Amanda Bird Hoffert Gilmartin, Serdar H Ural, John T Repke
Journal: Diabetes Care (1993)

DefinitionGestational diabetes mellitus is defined as carbohydrate intolerance of variable severity with onset or first recognition during pregnancy (1).The definition applies whether insulin is used for treatment or the condition persists after pregnancy but does not exclude the possibility that the glucose intolerance may have antedated the pregnancy.