tbuckley/cabot-search huggingface dataset:
Simple and Sensitive Point‐of‐Care Bioassay System Based on Hierarchically Structured Enzyme‐Mimetic Nanoparticles
Authors: Miju Kim, Min Su Kim, Soon Ho Kweon, Sanha Jeong, Mi Hyun Kang, Moon Il Kim, Jinwoo Lee, Junsang Doh
Journal: Advanced Healthcare Materials (2015)

An enzyme-mimetic nanoparticle-based point of care bioassay device is developed for rapid and sensitive detection of analytes. Digital images acquired by smart cellular phones allow quantifying the amounts of analytes. Using this new device, quantitative analysis of liquid sample is performed within 15 min with an order of magnitude enhancement of sensitivity compared with conventional Au nanoparticle-based devices. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
The challenge of integrating genetic medicine into primary care
Authors: J. Emery
Journal: BMJ (2001)

The likely increases in availability of DNA based tests and demand by patients for genetic information and advice mean that primary care practitioners will need to become genetically literate. 1 2Genetic medicine is already beginning to enter the realms of primary care through the availability of testing for predisposition to certain cancers and carrier screening and diagnostic tests for common recessive disorders such as cystic fibrosis and hereditary haemochromatosis.For the near future these issues will probably remain the focus of genetic medicine in primary care, but this could shift if pharmacogenetic research fulfils even some of its early promises.We discuss the implications of genetic advances for primary care, how genetic medicine could be integrated into primary care, and the skills that primary care practitioners will need to provide advice. MethodsWe searched Medline and Embase for relevant papers, combining terms relating to primary care and clinical genetics.We included papers identified from a previous systematic review of primary research on the role of family practice in genetics 3 and relevant papers published subsequently.The papers were heterogeneous in their focus and methods, and, when appropriate, we state the evidence on which our statements are based.
Severe cardiomyopathy following treatment with the tumour necrosis factor‐α inhibitor adalimumab for Crohn's disease
Authors: Maximilian Y. Emmert, Sacha P. Salzberg, Lorenz S. Emmert, Sohil Behjati, Andre Plass, Christian Felix, Volkmar Falk, Juerg Gruenenfelder
Journal: European Journal of Heart Failure (2009)
TREATMENT OF LYMPHOGRANULOMA INGUINALE WITH BOUILLON FILTRATE
Authors: William A. Thomas
Journal: JAMA (1934)

In view of the chronicity of course and the various forms of treatment advised in lymphogranuloma inguinale, we feel justified in reporting a single case because of the unusual rapidity and completeness of healing that followed treatment with autogenous filtrate.  A man, aged 31, seen at the office in October, 1932, had a tender swelling in the right groin draining since an incision had been made seven weeks previously. This had begun nine weeks before, with a small firm lump in the right groin aching somewhat. During the subsequent two weeks it became larger and softer, turned red and was incised, draining thick yellow pus. Fever persisted for ten days, the patient feeling ill; it then improved for a time, but both local and general conditions became worse with increased activity. From five to six weeks preceding the onset, the patient noticed several small red pimples on the penis, which
William Leslie Logie, MD
Authors: E. H. Bensley
Journal: JAMA (1968)

To the Editor:—  I am seeking information about William Leslie Logie, the university's first graduate. We know remarkably little about William Logie. Our records show that he was born in Montreal in 1810, that he was an Anglican, that he attended lectures during five years, following studies under Dr. William Robertson, and that he received his medical degree May 24, 1833.  I would be most grateful for any help which readers ofThe Journalmight provide.
Disease-specific risk of venous thromboembolic events is increased in idiopathic glomerulonephritis
Authors: Sean J. Barbour, Allen Greenwald, Ognjenka Djurdjev, Adeera Levin, Michelle A. Hladunewich, Patrick H. Nachman, Susan L. Hogan, Daniel C. Cattran, Heather N. Reich
Journal: Kidney International (2011)
Histopathology of the Skin
Authors: R. K. Winkelmann
Journal: JAMA (1962)

This is a book that represents dermatology well to all physicians. Since its first edition, the book has been a standard source for the student of dermatology. The young dermatologist found rational classifications of cutaneous disease coupled with succinct clinical profiles. The pathology was clearly outlined and superbly illustrated. These qualities are maintained, but the new edition of 650 pages may no longer be considered a précis, for many subjects are treated fully as in a textbook. As an example of the evolution of this work, it might be noted that the section on genodermatoses was 16 pages long and contained 10 illustrations in the first edition; it now has 25 pages and 16 illustrations. Additional bibliography and the enlarged discussion of many subjects such as pustular and bullous dermatoses, epidermal tumors, the nevus, and vasculitis add much to the general utility of the work. It is possible to forecast
MILK TREATMENT OF DISEASE.
Authors: JAMES TYSON
Journal: JAMA (1884)

(Read before the Section on Practice of Medicine and Materia Medica of the American Medical Association, May, 1884.) In the course of a somewhat extended experience with what is known as the "milk cure," it has occurred to me that the growing popularity of this form of treatment, demanded, at this time, some systematic consideration of the principles and practice on which its use is justified, as well as an outline of the class of cases in which it may be expected to be of advantage. It is now a little more than eighteen years since Dr. Philip Karell read his paper on the "milk Cure" before the Medical Society of St. Petersburgh. To this paper I must refer for a large number of interesting historical facts, of both ancient and modern date, bearing on this subject, beginning with Hippocrates and coming down to the date of the paper itself
Abstract LB-189: Fbxo7 functions in the proliferation and maturation of hematopoietic cells
Authors: Suzanne J. Randle, El Kahina Meziane, Mikhail Lomonosov, Heike Laman
Journal: Cancer Research (2011)

Abstract The F-box protein Fbxo7 specifically enhances the levels of cyclin D-Cdk6 complexes, the predominant G1 kinase active in hematopoietic cells, which is a commonly activated oncogenic pathway in lymphoid neoplasms. In addition, Fbxo7 is itself abundantly expressed in high-grade B and T cell malignancies. Previously we have tested the ability of Fbxo7 to act as an oncogene in the hematopoietic compartment in adoptive transfer experiments using p53 null hematopoietic stem cells over expressing Fbxo7. Animals reconstituted with these cells develop T cell lymphoma, supporting the idea that Fbxo7 can have tumor promoting activity in vivo. However, in experiments presented here, to investigate the transforming capacity of Fbxo7 in B lymphocytes, we found that overexpression of Fbxo7 in the mouse pro-B cell line Ba/F3 did not transform cells nor enhance their proliferation. Instead, the stable reduction of Fbxo7 expression increased proliferation and decreased cell size, which were attributable to shortening of G1 phase, decreased p27 levels and increased Cdk2 activity. These results suggest that Fbxo7 normally restrains proliferation in pro-B cells. Reducing Fbxo7 expression also changed the cell surface marker phenotype to resemble a more immature state of B cell maturation. To investigate the activities of Fbxo7 in hematopoietic cells in a more physiological setting, we have generated an in vivo model of reduced Fbxo7 expression by engineering a mouse with an insertional mutation of the LacZ gene into the FBXO7 locus. Phenotypic characterization of these mice will also be presented, and these data support the idea that Fbxo7 regulates the proliferation and the maturation of different precursor cell populations, including pro-B cells and pro-erythroblasts. We propose that Fbxo7 has the capacity to function as a tumor suppressor and also as an oncogene depending on the cell type and its stage of differentiation. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr LB-189. doi:10.1158/1538-7445.AM2011-LB-189
Lymph node grading for staging of mycosis fungoides may benefit from examination of multiple excised lymph nodes
Authors: Debra L. Breneman, Uma S. Raju, John C. Breneman, Paul E. Steele, David W. McFadden, Hernani D. Cualing, Michael S. Nussbaum, Steven H. Swerdlow
Journal: Journal of the American Academy of Dermatology (2003)
Nephropathy Induced by D-Penicillamine
Authors: ISRAELI A. JAFFE
Journal: Annals of Internal Medicine (1968)

Two patients given D-penicillamine for rheumatoid arthritis and scleroderma developed renal damage. In one patient, a renal biopsy had been obtained before treatment was begun that permitted comparison with subsequent biopsies done after the toxicity appeared. The changes found by electron microscopy in both patients were those of membranous transformation-electron-dense deposits on the epithelial surface of the basement membrane and fusion of epithelial foot processes. Immunofluorescent staining was positive with antisera to IgG and complement. Both patients improved after the drug was discontinued, and the intensity of immunofluorescent staining diminished. The nephropathy is most likely due to an immunological mechanism.
Ventricular Diastolic Stiffness Predicts Perioperative Morbidity and Duration of Pleural Effusions After the Fontan Operation
Authors: Cara A. Garofalo, Santos E. Cabreriza, T. Alexander Quinn, Alan D. Weinberg, Beth F. Printz, Daphne T. Hsu, Jan M. Quaegebeur, Ralph S. Mosca, Henry M. Spotnitz
Journal: Circulation (2006)

Background— We validated the clinical relevance of ventricular stiffness by examining surgical morbidity in children with univentricular hearts undergoing Fontan operation. We hypothesized that ventricular stiffness affects Fontan morbidity, particularly duration of pleural effusions. Methods and Results— Sixteen children with right ventricular (RV) (n =11) or left ventricular (LV) (n =5) dominance were studied intraoperatively at a median age of 3.3 years (1.8 to 5.1). Transesophageal long-axis echocardiograms and ventricular pressure by micromanometer provided end-diastolic pressure (P) area (A) relations during initiation and conclusion of cardiopulmonary bypass. Curve fitting to the equation P=αe βA defined the ventricular stiffness constant, β. Changes in β and clinical correlations were examined. Ventricular stiffness increased after bypass in patients with complete pre-bypass and post-bypass data (n =11, P =0.023, mixed models methodology). Pre-bypass β correlated well with duration of chest tube (CT) drainage ( r =0.90, n =16), net perioperative fluid balance ( r =0.71, n=14), and length of stay (LOS) ( r =0.81, n =16). CT duration and LOS also correlated significantly with post-bypass β ( r =0.77 for both, n=11), but insignificantly with preoperative catheterization pressures. Conclusions— Intraoperative β predicts duration of CT drainage, net perioperative fluid balance, and LOS after the Fontan operation. These observations could improve risk stratification and clinical management of children at high-risk undergoing the Fontan operation.
Abstract P3-04-05: Hypermethylation and Downregulation of Glutathione Peroxidase-3 in Inflammatory Breast Carcinogenesis
Authors: SF Sabet, MM Mostafa, M El-Shinawi, D Peng, MA Nouh, W. El-Rifai
Journal: Cancer Research (2010)

Abstract Background: The breast tumor microenvironment is characterized by the release of endogenous reactive oxygen species (ROS), and has been suggested as being associated with disease aggressiveness. Normal cells have an antioxidant system that controls the balance between production and removal of oxygen radicals, thereby protecting against oxidative damage, such as the Glutathione peroxidases (GPXs) enzymes. Epigenetics and DNA methylation play important roles in several inflammatory disorders. We therefore, analyzed the promoter of GPX3 and found a dense CpG island closest to the transcription start-site. The aim of the present study is to 1) investigate GPX3 expression in breast carcinoma and normal breast tissues, 2) identify whether GPX3 is epigenetically regulated in breast carcinoma tissues versus normal tissue, and 3) compare the levels of expression of GPX3 in Inflammatory Breast Cancer (IBC) versus non-IBC tissues. Material and Methods: We enrolled 40 breast cancer patients with tumor mass range from 1.8-9cm (with median size 4.77 ± 3.9). Patients were sub-grouped as IBC (n = 20) or non-IBC (n = 20), healthy breast tissues from the same patients were used as control. Using real-time PCR and immunohistochemistry, we assessed the level of expression of GPX3 at mRNA and protein levels in breast cancer tissues versus control. To identify whether the CpG island of the GPX3 gene was epigenetically regulated, we analyzed the methylation profile of GPX3 in breast carcinoma versus normal tissues using DNA bisulfate treatment and methylation-specific PCR (MSP). Results: GPX3-mRNA expression was down regulated in breast cancer samples compared to control tissues. There was a significant decrease (P = 0.01) in the mRNA expression level of IBC compared to non-IBC carcinoma tissues. MSP showed that the GPX3 gene was hypermethylated in breast carcinoma tissues compared to control. Moreover, confirmatory semiquantitative immunohistochemical scoring revealed weak or negative immunostain of carcinoma tissues compared to healthy breast tissues. Conclusion: These preliminary data suggest that epigenetic inactivation of GPX3 is a frequent finding in inflammatory breast cancer. Silencing of GPX3 in IBC versus non-IBC carcinoma cells suggested that GPX3 may be critical in the development and progression of IBC. To our knowledge, this is first study to test the role of GPX3 in breast cancer. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr P3-04-05.
"DOES PRIMARY TUBERCULOUS INFECTION AFFORD ADEQUATE PROTECTION AGAINST CONSUMPTION?"
Authors: D. O. N. Lindberg
Journal: JAMA (1933)

To the Editor:  —InThe Journal, April 8, appeared an article with this title by Dr. Chester A. Stewart of Minneapolis. Any attempt at evaluating the interesting material presented should not overlook the fact that certain groups have parted ways with the fundamentalists in phthisiology by rejoining the ranks left meager by the virtual abandonment of the contagionist's attitude toward tuberculosis of a few decades ago.  In view of the continuation of the downward trend, since the late period of the eighteenth century, of the mortality curve from tuberculosis, I believe that it is an unwarranted nullification of the program of public education, from which so much benefit has been derived, to propagandize in favor of the high contagiousness of tuberculosis, especially since there are many of that school who find it necessary or expedient to teach that primary infection by tubercle bacilli, as manifested by the cutaneous allergic response
Mst1 and Mst2 Maintain Hepatocyte Quiescence and Suppress Hepatocellular Carcinoma Development through Inactivation of the Yap1 Oncogene
Authors: Dawang Zhou, Claudius Conrad, Fan Xia, Ji-Sun Park, Bernhard Payer, Yi Yin, Gregory Y. Lauwers, Wolfgang Thasler, Jeannie T. Lee, Joseph Avruch, Nabeel Bardeesy
Journal: Cancer Cell (2009)
A Cost-effectiveness Analysis of Screening and Treatment for Chlamydia trachomatis Infection in Asymptomatic Women
Authors: Mehmet Genc
Journal: Annals of Internal Medicine (1996)

To assess the cost-effectiveness of identifying and treating asymptomatic female carriers of Chlamydia trachomatis.Cost-effectiveness analysis based on previously reported cohort analytic studies and average salaries and costs of medical care in Sweden.Women attending youth, family planning, and gynecology clinics.1000 women and their male sex partners.Screening with tissue cell culture, confirmed enzyme immunoassay, and DNA amplification assays based on either polymerase chain reaction or ligase chain reaction was compared with no screening (no treatment and no tracing of sexual contacts). The effect of antibiotic regimens on the outcome of the screening strategies was also evaluated.When the prevalence of chlamydial infection exceeded 6%, screening of women with DNA amplification assay and treatment of positive patients with a single oral dose of azithromycin given under supervision in the clinic was the most cost-effective intervention strategy. At greater prevalences, screening with enzyme immunoassay also generated savings and improved the cure rates compared with no screening, but such screening was less cost-effective than screening with a DNA amplification assay. Compared with no intervention, tissue cell culture is cost-effective only when the prevalence of infection is greater than 14%. Compared with the azithromycin regimen, the standard 7-day, twice-daily doxycycline regimen resulted in significantly lower cure rates because of patients' poor compliance with this regimen.For asymptomatic female carriers of C. trachomatis, screening with a DNA amplification assay combined with the single-dose azithromycin treatment of positive patients is the most cost-effective strategy when the prevalence is 6%. When the prevalence is lower than 6%, the decision to choose a competing strategy depends on the physician's view of the value of preventing an illness caused by untreated chlamydial infection.
Integration of donor mesenchymal stem cell-derived neuron-like cells into host neural network after rat spinal cord transection
Authors: Xiang Zeng, Xue-Cheng Qiu, Yuan-Huan Ma, Jing-Jing Duan, Yuan-Feng Chen, Huai-Yu Gu, Jun-Mei Wang, Eng-Ang Ling, Jin-Lang Wu, Wutian Wu, Yuan-Shan Zeng
Journal: Biomaterials (2015)
The Infant's Serologic Response to Live Poliomyelitis Virus Vaccine
Authors: Randolph Batson
Journal: JAMA (1962)

Attenuated poliovirus vaccine (Sabin) was given to 90 young infants by 5 schedules of administration in order to determine the degrees of immunologic response and the influence of placentally transferred passive antibodies. Group 1 received 3 vaccine types at 6-week intervals as monovalent vaccine; Group 2 received 3 types administered together on 3 separate occasions; Groups 3, 4, and 5 received the 3 types as a single dose at 6 weeks, 12 weeks, and 18 weeks of age, respectively. A sixth group, as a contratest, did not receive vaccine. Groups 1 and 2 developed the highest antibody levels. High levels of passive antibody of maternal origin did not interfere with production of active antibody.
Self monitoring of glucose by people with diabetes: evidence based practice
Authors: M. Gallichan
Journal: BMJ (1997)

The inappropriate use of self monitoring of glucose is wasteful of NHS resources and can cause psychological harm. Although a few patients find that self monitoring enables them to understand and take control of their diabetes, many people with diabetes are performing inaccurate or unnecessary tests. There is no convincing evidence that self monitoring improves glycaemic control, nor that blood testing is necessarily better than urine testing. It may be appropriate for some patients not to monitor their own glucose but to rely instead on regular laboratory estimations of glycaemic control. Glucose self monitoring should be performed only when it serves an identified purpose. It is widely assumed that glucose self monitoring, preferably of blood glucose concentrations, is desirable or even essential for everyone with diabetes. It is common for patients who have previously tested their urine, or have done no glucose monitoring at home, to be taught to measure their blood glucose when they are admitted to hospital. In the community too, patients are often encouraged to monitor their blood glucose, and newly diagnosed patients of all ages are usually taught to measure their blood glucose concentrations. Self monitoring can sometimes be useful, but evidence is mounting that its indiscriminate use is of questionable value. In 1995, Pounds 42.6 million was spent on home monitoring of glucose in the United Kingdom (Intercontinental Medical Statistics, personal communication). Is this enormous cost justified? Is blood testing necessarily better than urine testing? Is glucose self monitoring always necessary, or is it sometimes a waste of time and money? Are recommendations for self monitoring based on sound evidence?
Perivascular Inflammation After Balloon Angioplasty of Porcine Coronary Arteries
Authors: Ei-ichi Okamoto, Tracey Couse, Hector De Leon, Jakob Vinten-Johansen, Richard B. Goodman, Neal A. Scott, Josiah N. Wilcox
Journal: Circulation (2001)

Background Inflammation has been suggested to play a role in vascular lesion formation after angioplasty. Whereas previous studies have focused on inflammatory reactions in the intima and media, less attention has been paid to adventitial and perivascular responses and their potential role in vascular remodeling. Methods and Results Balloon overstretch injury of porcine coronary arteries was performed with standard clinical angioplasty catheters. Vessels were examined from 0.5 hour to 14 days after injury by immunohistochemistry and in situ hybridization (ISH) for neutrophil and macrophage markers, cell adhesion molecules (P-selectin, E-selectin, and vascular cell adhesion molecule-1), and neutrophil-specific CXC chemokines (alveolar macrophage–derived neutrophil chemotactic factor [AMCF]-I/interleukin-8 and AMCF-II). Neutrophils accumulated in the adventitia surrounding the injury site from 2 hours to 3 days, followed by macrophages from 1 to 7 days after angioplasty. Inflammation was associated temporally with the expression of mRNAs encoding cell adhesion molecules and chemokines. The main inflammatory and proliferative foci were not limited to the adventitia but rather extended many millimeters away from the injured vessel throughout the surrounding adipose and myocardial tissues. Conclusions Inflammatory responses after angioplasty of porcine coronary arteries occurred throughout the entire perivascular tissue. We hypothesize that perivascular inflammatory cells play a role in the recruitment and/or proliferation of adventitial myofibroblasts, possibly through the release of reactive oxygen species and/or cytokines, and thus contribute to vascular remodeling associated with postangioplasty restenosis.
A New Method of Treatment of Inflammatory Lesions of the Female Urethra
Authors: Charles Rieser
Journal: JAMA (1968)

The female urethra is the homologue of the posterior urethra of the male. Vestigial remnants of the prostatic ducts and glands are part of the anatomy of the normal female urethra. These structures are predisposed to infection, both acute and chronic. A simple and successful method of treatment is aimed at complete eradication of the responsible primarily infected paraurethral ducts and glands and the dependent secondary pathologic changes of contiguous structures. Symptomatic relief, elimination of infection, and absence of recurrence in 206 patients have been impressive.