tbuckley/cabot-search huggingface dataset:
Abstract 13798: Ablation of the Hypertension Candidate Gene ATP2B1 Results in Increased Blood Pressure and Cardiac Hypertrophic Remodeling
Authors: Sally K Hammad, Min Zi, Sukhpal Prehar, Robert Little, Ludwig Neyses, Elizabeth J Cartwright
Journal: Circulation (2014)

Introduction: Hypertension is a major risk factor for cardiac hypertrophy and heart failure. Genome wide association studies have recently identified single nucleotide polymorphisms in ATP2B1 , the gene encoding the calcium extrusion pump, plasma membrane calcium ATPase (PMCA1), as having a strong association with hypertension risk. Hypothesis: PMCA1 plays an important role in regulation of blood pressure and protection against hypertension and cardiac hypertrophy. Aims: We aim to examine whether there is a functional link between PMCA1 and blood pressure regulation, and the development of hypertension. And to determine the impact this link may have on cardiac structure and function. Methods and Results: To study the role of PMCA1 we generated a global PMCA1 heterozygous knockout mouse (PMCA1 Ht ). PMCA1 Ht mice had 46% to 52% reduction in PMCA1 protein expression compared to the WT, in aorta, heart, kidney and brain. To study the mice under hypertensive stress conditions, 3 month old PMCA1 Ht and wild type (WT) mice were infused via minipump with angiotensin II (1mg/Kg/daily) or water as a control. Upon angiotensin treatment, PMCA1 Ht mice showed a significantly greater increase in systolic (62.24±3.05 mmHg) and diastolic pressure (52.68±4.67 mmHg), in comparison to the WT (33.37±2.91 mmHg and 23.94±4.56 mmHg, respectively), P&lt;0.001, n=12. Moreover, PMCA1 Ht mice showed a significantly greater hypertrophic response as indicated by a greater heart weight to tibia length ratio, cardiomyocyte cell size (410±18.7 μm 2 ), compared to WT mice (340.4±9.8 μm 2 ), and increased expression of B-type natriuretic peptide (BNP), 2.36 ± 0.25 fold change, n =5-6, P&lt; 0.01. Echocardiography showed no significant changes between PMCA1 Ht and WT mice, in heart rate, and in cardiac function, as indicated by fractional shortening and ejection fraction. In addition, PMCA1 Ht mice showed no sign of lung congestion as indicated by lung weight to body weight ratio. Conclusion: ATP2B1 deletion leads to increased blood pressure and cardiac hypertrophy. This provides functional evidence that PMCA1 is involved in blood pressure regulation and protects against the development of hypertension and cardiac hypertrophy.
Changing Continuing Medical Education
Authors: Ronald M. Cervero
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Theory-testing experiments in the economics laboratory
Authors: Anthony S. Gillies, Mary Rigdon
Journal: Behavioral and Brain Sciences (2001)

Features of experimental design impose auxiliary hypotheses on experimenters. Hertwig &amp; Ortmann rightly argue that the ways some variables are implemented in psychology cloud results, whereas the different implementations in economics provide for more robust results. However, not all design variables support this general conclusion. The repetition of trials may confuse results depending on what theory is being tested. We explore this in the case of simple bargaining games.
Evaluation and Management of Chylous Ascites
Authors: OLIVER W. PRESS
Journal: Annals of Internal Medicine (1982)

Twenty-eight cases of chylous ascites occurring over the past 20 years are analyzed for clinical presentation, cause, yield of diagnostic procedure used, and response to therapy. Malignancies were responsible for 21 of 24 adult cases of chyloperitoneum, with lymphomas predominating (13 cases). The dismal prognosis in adult cases (12 patients died within 3 months) shows the need for appropriate diagnostic assessment including early lymph node biopsy or laparotomy, or both, when indicated. Surgery, chemotherapy, or radiation therapy should be instituted promptly except in cases resulting from surgical trauma to lymphatics, which frequently resolve with conservative management. Three of the four pediatric cases of chylous ascites resulted from congenital lymphatic anomalies; the fourth case resulted from operative trauma. Aggressive diagnostic and therapeutic interventions are not warranted in childhood cases of chylous ascites until conservative management (paracentesis, low-fat diet, medium-chain triglyceride supplementation) has failed; neoplasia is rarely implicated and many cases resolve within a few months.
422. Optimization of AAV Vector Production by Transient Transfection
Authors: Paul P. A. Oranje, Peter Verheesen, Dennis Verbart, Yvonne Mijnsbergen, Hans J. W. Haard, Pim Hermans, Mark R. ten Haaft, Jaap Twisk, Paul A. Dijkhuizen, Wim T. J. M. C. Hermens, Janneke J. M. Meulenberg
Journal: Molecular Therapy (2004)

Recombinant adeno-associated virus (rAAV) vectors hold promise for human gene therapy. Although most preclinical studies and current phase I clinical trials use vectors derived from rAAV serotype 2, other serotypes have been identified which transduce certain cell types more efficiently. The availability of sufficient quantity and quality of rAAV vectors is a key element in obtaining reliable and reproducible data and is essential for the transition to human clinical trials. Furthermore, to allow a fair comparison of the transduction efficiencies of rAAV vectors of different serotypes in vitro and in vivo, their quality and purity should be similar. This stresses the need for a universal purification method for different AAV serotypes. The aim of this study was to develop such a method, making use of single-domain antibody fragments from species of Camelidae. These antibodies have excellent binding characteristics, unusual high physical and thermal stability due to their single domain nature, and are efficiently produced in S.cerevisiae. A single domain antibody fragment with affinity for rAAV2 was selected from a naive Llama phage library. The selected antibody fragment was coupled to chromatography resin and displayed high affinity for rAAV vectors pseudotyped with capsids of serotype 1, 2, 3, and 5 when tested in small scale chromatography experiments. These results indicated that the single chain antibody fragment recognized a conserved epitope in the capsid proteins of these serotypes. The process was further optimized and scaled up to allow purification of clinical grade rAAV1 vector. This resulted in a simple, reproducible, and efficient purification process providing highly purified rAAV1 with recoveries of approximately 50%. Characterization of the purified rAAV1 in vitro and in vivo, demonstrated that this vector induced good levels of transgene expression. From this study we conclude that single domain antibody fragments are extremely powerful for efficient purification of rAAV vectors and will significantly improve the characterization and comparison of rAAV vectors of different serotypes as vectors for gene therapy applications.
Restricted coronary flow reserve in patients with mitral regurgitation improves after mitral reconstructive surgery11There are no financial or other relations that could lead to a conflict of interest.
Authors: Takashi Akasaka, Kiyoshi Yoshida, Takeshi Hozumi, Tsutomu Takagi, Shuichiro Kaji, Takahiro Kawamoto, Yoshiaki Ueda, Yukikatsu Okada, Shigefumi Morioka, Junichi Yoshikawa
Journal: Journal of the American College of Cardiology (1998)
Vulvovaginal photodynamic therapy vs. topical corticosteroids in genital erosive lichen planus: a randomized controlled trial
Authors: A.L.O. Helgesen, T. Warloe, A.H. Pripp, R. Kirschner, Q. Peng, T. Tanbo, P. Gjersvik
Journal: British Journal of Dermatology (2015)

Journal Article Vulvovaginal photodynamic therapy vs. topical corticosteroids in genital erosive lichen planus: a randomized controlled trial Get access A.L.O. Helgesen, A.L.O. Helgesen Norwegian National Advisory Unit on Women's Health Oslo University Hospital N‐0424 Oslo NorwayDepartment of Dermatology Oslo University Hospital N‐0424 Oslo NorwayInstitute of Clinical Medicine Oslo University Hospital N‐0424 Oslo Norway Correspondence Anne Lise Ording Helgesen. E‐mail: anne.lise.helgesen@ous-hf.no Search for other works by this author on: Oxford Academic Google Scholar T. Warloe, T. Warloe Department of Internal Medicine Oslo University Hospital N‐0424 Oslo Norway Search for other works by this author on: Oxford Academic Google Scholar A.H. Pripp, A.H. Pripp Oslo Centre of Biostatistics and Epidemiology Research Support Services Oslo University Hospital N‐0424 Oslo Norway Search for other works by this author on: Oxford Academic Google Scholar R. Kirschner, R. Kirschner Department of Gynaecology Oslo University Hospital N‐0424 Oslo Norway Search for other works by this author on: Oxford Academic Google Scholar Q. Peng, Q. Peng Department of Pathology Oslo University Hospital N‐0424 Oslo Norway Search for other works by this author on: Oxford Academic Google Scholar T. Tanbo, T. Tanbo Institute of Clinical Medicine Oslo University Hospital N‐0424 Oslo NorwayDepartment of Gynaecology Oslo University Hospital N‐0424 Oslo Norway Search for other works by this author on: Oxford Academic Google Scholar P. Gjersvik P. Gjersvik Department of Dermatology Oslo University Hospital N‐0424 Oslo NorwayInstitute of Clinical Medicine Oslo University Hospital N‐0424 Oslo Norway Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 173, Issue 5, 1 November 2015, Pages 1156–1162, https://doi.org/10.1111/bjd.14033 Published: 01 November 2015
Abstract 909: Natural History of Coronary Artery Lesion Morphology Assessed by Serial VH-IVUS Tissue Characterization
Authors: Takashi Kubo, Akiko Maehara, Gary S Mintz, Hiroshi Doi, Kenichi Tsujita, Junqing Yang, Jian Liu, Carlos Oviedo, Anthony Tam, Harpreet Bharaj, Richard Abendroth, Sinan Biro, Rasha Aaskar, Celia Castellanos, Lokesh Dani, Jeffrey W Moses, Geoffrey Vince, Pauliina Margolis, James R Margolis, Gregg W Stone, Martin B Leon
Journal: Circulation (2008)
Abstract 4551: Emerging effects of Chinese herbal medicines on human cancer cells proliferation
Authors: Xufei Zhang, Hao Li, Herschel Angela Espiritu, Anita Kim, Li Zhong
Journal: Cancer Research (2014)

Abstract Chinese herbal medicine is a major aspect of traditional Chinese medicine, which focuses on restoring a balance of energy, body, andspirit to maintain health rather than treating a particular disease or medical condition. Some of Chinese herbs have shown therapeutic effects in cancer patients. Yunnan Baiyao (White Medicine from Yunnan, YNBY) and Snow daisy are popular Chinese herbs for routine clinical usage. YNBY is a Chinese herbal medicinal powder used to stop bleeding, reduce swelling and improve circulation in traumatic injuries. While Snow daisy has effects on maintaining blood pressure and reducing coronary heart diseases and inflammation when consumed in form of tea. Although it has been hypothesized that both YNBY and Snow daisy flower tea (SDFT) may help to prevent tumor proliferation and even reduce tumor growth, yet no systematic study and analysis have been carried out. Therefore, in this study the effects of YNBY and SDFT on tumor cell proliferation were evaluated and the signal transduction pathways mediating the effects were also determined. We tested tumor cells (lung, breast and oral cancer cell lines) and normal human fibroblast cells with different YNBY and SDFT dilution in cultured medium. Then the proliferation ability of these treated cells was tested by MTT assay and visualized via Dil (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine Perchlorate) staining. Subsequently, several apoptosis factors (Fas pathway related, i.e., Fas/CD95, Fas Ligand, Caspase-8) which may be secreted by YNBY and SDFT treated tumor cells were tested using ELISA and Western Blot. The results showed that YNBY and SDFT can significantly inhibit lung tumor cells proliferation when its concentration was above 0.4mg/mL and 2mg/mL, respectively (P&amp;lt;0.01). The data indicated that YNBY could induce lung tumor cells apoptosis through Fas related pathway. The expression of Fas/CD95 and caspase-8 in treated lung tumor cells was much higher than which in control groups (P&amp;lt;0.01) suggesting that YNBY and SDFT may provoke tumor cells death by inducing cell apoptosis through Fas pathway. YNBY and SDFT may constitute potential therapeutic effects for lung cancer patients. Citation Format: Xufei Zhang, Hao Li, Herschel Angela Espiritu, Anita Kim, Li Zhong. Emerging effects of Chinese herbal medicines on human cancer cells proliferation. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4551. doi:10.1158/1538-7445.AM2014-4551
A novel nicastrin mutation in a large Chinese family with hidradenitis suppurativa
Authors: T. Jiao, H. Dong, L. Jin, S. Wang, J. Wang
Journal: British Journal of Dermatology (2013)

Journal Article A novel nicastrin mutation in a large Chinese family with hidradenitis suppurativa Get access T. Jiao, T. Jiao Department of Dermatology, Ningbo No. 2 Hospital, Ningbo 315010, China Search for other works by this author on: Oxford Academic Google Scholar H. Dong, H. Dong Shanghai‐MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai 201203, China Search for other works by this author on: Oxford Academic Google Scholar L. Jin, L. Jin Shanghai‐MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai 201203, China Search for other works by this author on: Oxford Academic Google Scholar S. Wang, S. Wang Shanghai‐MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai 201203, China Shengyue Wang, E‐mail: wangsy@chgc.sh.cn; Jinyan Wang, E‐mail: nbwangjinyan@126.com Search for other works by this author on: Oxford Academic Google Scholar J. Wang J. Wang Department of Dermatology, Ningbo No. 2 Hospital, Ningbo 315010, China Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 168, Issue 5, 1 May 2013, Pages 1141–1143, https://doi.org/10.1111/bjd.12135 Published: 01 May 2013
TEL/AML-1 dimerizes and is associated with a favorable outcome in childhood acute lymphoblastic leukemia
Authors: TW McLean, S Ringold, D Neuberg, K Stegmaier, R Tantravahi, J Ritz, HP Koeffler, S Takeuchi, JW Janssen, T Seriu, CR Bartram, SE Sallan, DG Gilliland, TR Golub
Journal: Blood (1996)
Blast transformation in chronic myelomonocytic leukemia: Risk factors, genetic features, survival, and treatment outcome
Authors: Mrinal M. Patnaik, Emnet A. Wassie, Terra L. Lasho, Curtis A. Hanson, Rhett Ketterling, Ayalew Tefferi
Journal: American Journal of Hematology (2015)

Among 274 patients with chronic myelomonocytic leukemia (CMML) and followed for a median of 17.1 months, blast transformation (BT) occurred in 36 (13%). On multivariable analysis, risk factors for BT were presence of circulating blasts (HR 5.7; 95% CI 2.8-11.9) and female gender (HR 2.6; 95% CI 1.3-5.1); the results remained unchanged when analysis was restricted to CMML-1. ASXL1/SRSF2/SF3B1/U2AF1/SETBP1 mutational frequencies were not significantly different between time of CMML diagnosis and BT. Median survival post-BT was 4.7 months (5-year survival 6%) and better with allogeneic stem cell transplant (SCT) (14.3 months vs. 4.3 months for chemotherapy vs. 0.9 months for supportive care; P = 0.03). Neither karyotype nor mutational status was independently associated with risk of BT or post-BT survival. We conclude that female patients with CMML and those with circulating blasts are at a higher risk of BT. Post-BT survival is dismal and our observations suggest consideration of allogeneic SCT prior to BT.
Abstract 18714: Role of 123 Iodine Metaiodobenzylguanidine Imaging in Prediction of Arrhythmic Events in Heart Failure Patients Candidate to ICD
Authors: Noemi Bruno, Nicolò Salvi, Paola Scarparo, Camilla Calvieri, Alessandra Armato, Alessandra Cinque, Pasqualina Bruno, Massimo Mancone, Giuseppe De Vincentis, Francesco Fedele
Journal: Circulation (2014)

Background: According to guidelines, implantable cardioverter defibrillator (ICD) is recommended in prevention of sudden cardiac death (SCD) in heart failure (HF) patients (pts). Guidelines have several limitations because ICD indication is based mainly on left ventricular ejection fraction (EF). Recent data showed that, independently from EF, 123-iodine metaiodobenzylguanidine imaging (123-I MIBG) could help to identify HF pts at high risk of SCD [heart/ mediastinum (H/M) ratio ≤1.6 and a summed score (SS) &gt; 26], who may benefit of ICD. Aim: Our aim is to assess, in a real world registry, the role of 123-I MIBG for the prediction of ventricular tachyarrhythmia (VT) causing appropriate ICD therapy in HF pts. Methods: We consecutively enrolled 97 patients admitted to our hospital with diagnosis of HF, left ventricular ejection fraction (LVEF) ≤35% and indication to ICD. All patients underwent MIBG imaging. The patients were classified into two groups: Group 1 with H/M≤1.6 , SS&gt; 26; Group2 with H/M&gt;1.6, SS &lt;26. All patients underwent 1 year follow-up. Results: 65 pts were included in group 1 and 32 pts in group 2. All baseline characteristics were similar in 2 groups apart from the etiology (table 1). In group 1, H/M ratio was 1.37±0.3 vs 1.8 ± 0.2 in group 2 (p=0.0002); SS was 37.5± 9.7 vs 16 ±6 in group 2 (p = 0.0001). At 1 year follow-up VTs causing appropriate ICD therapy in group 1 were 13.4% vs 1.28% in group 2(p=0.02); overall cardiac events were in group 1 16.4 % vs 1.92% in group 2 (p=0.02). Conclusion: Our results suggest that 123 I-MIBG can identify patients at increased risk for arrhythmic death and can be useful in the decision-making of ICD implantation independently from ejection fraction.
Rapid chest compression and flow limitation
Authors: J Hammer, CJ Newth
Journal: European Respiratory Journal (1995)

re es ss si io on n a an nd d f fl lo ow w l li im mi it ta at ti io on n6.It has been demonstrated that control over volume history reduces the intrasubject variability of flow measurements by RCT [6].It is possible that the breath-stacking technique reduced the scatter of the flow data.This remains unclear because coefficients of variation of repeated measurements are not reported.All these factors allow forced expiratory flows to differ among different manoeuvres even when wave speed (flow-limitation) conditions are achieved by each technique, and could explain the reported flow differences.Unfortunately, this study cannot resolve the ongoing flowlimitation debate in RCT, but demonstrates that standardization of forced expiratory manoeuvres is essential for flow comparison; a fact well-known in adult lung function laboratories.
Abstract 11285: Heart Failure Rescued by Pharmacological Inhibition of Type 5 Adenylyl Cyclase
Authors: Kosaku Iwatsubo, Erdene Baljinnyam, Lo Lai, Chull Hong, Shumin Gao, Lin Yan, Dorothy Vatner, Junichi Sadoshima, Stephen Vatner, Yoshihiro Ishikawa
Journal: Circulation (2010)
Ablation of PDK1 in pancreatic β cells induces diabetes as a result of loss of β cell mass
Authors: Naoko Hashimoto, Yoshiaki Kido, Tohru Uchida, Shun-ichiro Asahara, Yutaka Shigeyama, Tomokazu Matsuda, Akihiko Takeda, Daisuke Tsuchihashi, Akihiko Nishizawa, Wataru Ogawa, Yoshito Fujimoto, Hitoshi Okamura, Karen C Arden, Pedro L Herrera, Tetsuo Noda, Masato Kasuga
Journal: Nature Genetics (2006)
Abstract 16008: Improved Safety and Efficacy of a Novel Dual cRGD- and Everolimus-Coated Coronary Stent Compared to Everolimus-Eluting Stents in the Porcine Coronary Model
Authors: Felix Vogt, Sebastian Mause, Jörg Schröder, Elisa Liehn, Nicole Krott, Nikolaus Marx, Rüdiger Blindt
Journal: Circulation (2014)

Background: Re-endothelialization after drug-eluting stent implantation is delayed. Integrin-binding cyclic Arg-Gly-As p (cRGD) peptide-loaded stents offer potential for enhanced early and late endothelial recovery by endothelial progenitor cell (EPC) anchorage. Here, a novel dual cRGD and everolimus-eluting stent was compared to an everolimus-eluting stent. Methods: The porcine coronary overstretch model was employed for analysis of Multi-Link Tetra bare metal stents with or without a specific conformal, mechanically robust polymer coating based on an aromatic polyetherurethaneurea with a soft segment of polytetramethyleneoxide and a hard segment of diphenylmethane diisocyanate and mixed diamines covered by a control-release layer. Stents were coated with immobilized cRGD, everolimus or cRGD+everolimus on the biostable PEA-40 polymer surface coating. Isolated porcine EPCs were labeled with cell tracker and infused into coronary arteries immediately after stent implantation. cRGD or everolimus recovered mass was quantified, re-endothelialization was assessed by scanning electron microscopy and area stenosis was quantified. Results: cRGD and everolimus recovered mass from PEA-40 cRGD/everolimus stents possessed a slow release profile. Marked EPC recruitment to stents was evidenced after intracoronary infusion of fluorescence-labeled EPCs. In cRGD+everolimus loaded stents, in-stent cross sectional stenosis was reduced and re-endothelialization above stent struts was accelerated (p&lt;0.05 for both) while re-endothelialization between struts was similar (p&gt;0.05) as compared to both everolimus-loaded and bare-metal stents. Conclusions: PEA-40 is a reliable carrier for cRGD and everolimus; the novel PEA-40 dual cRGD and everolimus stent coating both everolimus-dependently decreases coronary stenosis as well as integrin-dependently accelerates stent strut re-endothelialization as compared to everolimus-eluting stents in the porcine coronary overstretch model. Therefore, the concerted acceleration of vascular healing by facilitated homing of circulating EPCs and distinct antiproliferatory properties of everolimus might transfer to an improvement of stent safety while stent efficacy is maintained.