tbuckley/cabot-search huggingface dataset:
452. Adenoviral Gene Therapy Using CRAd Targeted to Cells with Hyperactive Wnt/β-Catenin Signaling
Authors: Atique Ahmed, Jian Qiao, Jesus Moreno, Richard G. Vile
Journal: Molecular Therapy (2004)

During cell division, the genomic methylation pattern is faithfully maintained by DNA methyltransferase-1 (DNMT-1), which is responsible for transferring a methyl group to 5-position of the cytosine ring. Methylation occurs mostly during DNA replication and the expression of DNMT-1 is tightly regulated with the cell cycle. Previously, it has been demonstrated that although both cycling and growth-arrested cells transcribe DNMT-1 mRNA at a similar rate, the mRNA is detectable only in the cycling cells. The 3′ untranslated region (3′ UTR) of DNMT-1 mRNA contains copies of adelylated-and uridylate-rich elements (AREs), which can regulate stability of its own message as well as a heterologus β-globin mRNA in a growth-dependent manner. Because cancer cells proliferate much faster than most normal adult somatic cells, we hypothesized that cancer cell selective mRNA-stabilization and transgene expression could be achieved by using the DNMT-1 3 ′UTR ligated to a therapeutic gene or viral replicative gene. To test the hypothesis, the human DNMT-1 3′UTR (5090–5408) was amplified by RT-PCR from RNA prepared from the human melanoma cancer Mel-624 cell line and inserted into the luciferase reporter plasmid pGL-3 promoter. The effect of the DNMT-1 3′ UTR insertion was evaluated by comparing the relative luciferase activity in tumor (prostate cancer:PC-3,LnCap; glioma: U118, U87, U251; melanoma: Mel624 and Mel888; colorectal cancer: HCT116) and three normal cell lines (BEAS-2B, HUVAC, HTB-125). In the presence of DNMT1 3′ UTR, luciferase activity was 5-7 fold higher in all the tumor cell lines compared to the normal cells. Therefor, we generated a conditionally replicating adenovirus where the viral E1A gene, which is essential for viral replication, is ligated to the 318bp 3′-untranslated region of the DNMT-1 mRNA (AdDNMT1). In vitro studies with AdDNMT1 virus on a variety of normal and tumor cell lines have shown that productive infection and viral replication is restricted to rapid growing tumor cells. Early (E1A), late (fiber) viral protein expression and lytic replication was not observed in normal primary cell lines tested. These result supports the possibility of targeting tumor cells by using DNMT-1 3′UTR in the context of abnormal growth.
Ventricular enlargement in teenage patients with schizophrenia spectrum disorder
Authors: S. C. Schulz, M. M. Koller, P. R. Kishore, Robert M Hamer, J. J. Gehl, R. O. Friedel
Journal: American Journal of Psychiatry (1983)

Recent research has demonstrated statistically significant differences between the ventricular-brain ratios (VBRs) of schizophrenic patients and control subjects. In this study the VBRs of teenage schizophrenic/schizophreniform patients (N = 15) and borderline patients (N = 8) were measured and compared with those of controls of similar ages (N = 18). The schizophrenic group had significantly larger ventricles than the other two groups (p less than .0001). These findings support the hypothesis of previous investigators that ventricular enlargement is present early in the course of schizophrenia.
Pulsed Dye Laser Treatment of Acne Vulgaris—Reply
Authors: Jeffrey S. Orringer, Sewon Kang, Timothy M. Johnson, Gary J. Fisher, John J. Voorhees
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Satisfaction with nurse specialists in breast care clinics
Authors: J M Dixon, J Lamb, G Stones, A Rahman, D Mitchell, M Bramley, G J Byrne, N J Bundred, L Garvican, P Littlejohns, N P M Sacks
Journal: BMJ (1998)

Editor—Garvican et al conclude that since the results of the fine needle aspiration obtained by clinical nurse specialists in their breast clinic were better than those obtained by other clinicians the nurses’ clinical expertise compared favourably with that of other clinicians.1 In a recent four month survey of fine needle aspiration cytology in our unit, 86 (20%) of 432 aspirates were classed as inadequate (C1). This is significantly lower than the rate of 276 (33.5%) of 825 samples (P<0.0001) classed as inadequate in Garvican et al’s paper. Two thirds of the aspirations of palpable lesions in Edinburgh were performed by consultants; their rate of inadequate samples was 38/233 (16%). This is significantly lower than the 114 (32%) of 362 samples taken by the nurses that were classed as inadequate (P<0.0001). The nurse specialists performed 362 (44%) of 825 aspirations in their clinic; in Edinburgh non-consultant clinicians performed 35 (28%) of 124 (P=0.012). These results confirm that the experience of the clinician performing an aspiration is an important factor in the success of the technique.2

In the breast clinic the ratio of benign samples to malignant samples was 5.1:1, while in Edinburgh it was significantly lower at 1.2:1 (P<0.0001). In Edinburgh all patients are seen by experienced consultant breast surgeons or senior doctors, and immediate access to mammography and ultrasonography is available during the clinic. This may explain the apparently better selection of patients for aspiration cytology, which is the most painful test performed in breast clinics.3

We recently introduced a “one stop” clinic in which women have immediate access to breast imaging and to the results of imaging and aspiration. We assessed the satisfaction of patients who required ultrasound imaging or aspiration with a questionnaire which was completed before leaving the clinic. Before the introduction of the new service only 50/125 (40%) women were completely satisfied with their visits; the most common complaint was about the delay in receiving test results. After the introduction of the one stop clinic, 80/114 (70%) indicated that they were completely satisfied. Reasons for a lack of complete satisfaction with the new service were all non-medical and included problems with car parking (24/114, 21%) and poor signposting to the clinic (11/114, 10%). Difficulties in parking increased patients’ anxiety, and anxiety relates to the pain experienced during investigations.3 In comparison with the breast clinic described by Garvican et al we perform fewer fine needle aspirations in cases of benign disease and obtain fewer inadequate samples. Far from cost benefits accruing from nurse led clinics there may be cost implications because of the extra aspirations performed and the higher rates of obtaining inadequate samples.
Increased exhaled nitric oxide predicts new-onset rhinitis in asymptomatic children
Authors: Andrei Malinovschi, Christer Janson, Pia Kalm-Stephens, Kjell Alving, Lennart Nordvall
Journal: European Respiratory Journal (2011)

Background: The fraction of nitric oxide in exhaled air (FeNO) is increased in rhinitis and asthma. Recent studies have reported that high FeNO levels in asymptomatic subjects can predict later onset of asthma. No studies have analysed if increased FeNO in asymptomatic subjects can predict later onset of rhinitis.

Aim: To investigate in a cohort of schoolchildren if increased FeNO levels at the age of 13-14 years predict new-onset of rhinitis within a 4-years period.

Methods: A total of 959 randomly selected schoolchildren, aged 13-14 years, answered questions on respiratory and allergy symptoms, family history of asthma. Lung function and FeNO were also measured at baseline. A follow-up with the same questions was performed four years later. After exclusion of subjects with asthma or rhinitis symptoms at baseline, 555 participants were eligible for the present study.

Results: Subjects with new-onset rhinitis (n=92) had a trend of higher FeNO than subjects who did not develop rhinitis (p=0.06). Increased FeNO predicted new-onset rhinitis in a multiple logistic regression model (p=0.009) and the risk of new-onset rhinitis was 2.4-fold (1.2, 4.4) elevated if FeNO > 90th percentile of all included subjects (n=555). A similar risk increase for new-onset rhinitis, 2.4 (1.2, 4.9) was found in subjects with no allergy symptoms and 2.3 (1.04, 5.1) after further excluding subjects with a family history of asthma.

Conclusion: Increased FeNO levels predicted new-onset rhinitis in this population-based study of schoolchildren. The predictive value in subjects without allergic symptoms or family history of asthma suggests that these children with increased FeNO should be tested for allergy and followed-up.
Efficacy of Sertraline in the Treatment of Children and Adolescents With Major Depressive Disorder
Authors: Maju Mathews, Manu Mathews, Joanne Mathews
Journal: JAMA (2004)

To the Editor: Dr Wagner and colleagues1 concluded that "sertraline is an effective, safe, and well-tolerated short-term treatment for children and adolescents with MDD [major depressive disorder]." We have some questions about their methods.
Hormone Therapy and Risk of Gynecologic Cancers—Reply
Authors: Garnet L. Anderson, Howard L. Judd, Andrew M. Kaunitz, S. Gene McNeeley, David H. Barad, James Liu, Ana Maria Lopez, Shirley A. A. Beresford, Mary Pettinger
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 17923: Hypoxia Inducible Factor-1{alpha} Dependent miR-210 is a Critical Regulator of Paracrine Activity in Preconditioned Mesenchymal Stem Cells for Angiogenic Growth Factor Expression
Authors: Shazia Durrani, Ha Won Kim, Rafeeq P Ahmed, Muhammad Ashraf, Husnain K Haider
Journal: Circulation (2010)
Ingested foreign bodies and societal wealth: three year observational study of swallowed coins
Authors: P G Firth, H Zheng, J A Biller
Journal: BMJ (2009)

To examine the relation between coins ingested by children and the Dow Jones Industrial Average.Observational study. Main outcome measures Total value of coins ingested and number of incidents of coins versus other objects swallowed, measured before and after the stock market crash of October 2008.Eighteen objects, including 11 coins, were ingested (NASDAQ (numismatic and sundry detritus acquired) composite of 18). The total value of the 11 coins swallowed was $1.03 (FTSE 100 (fraction of the US$ or 100 cents) index of 103). The pecuniary extraction ratio (PE ratio) was 0.57 (9/16). Comparing values for a period before and after October 2008, the mean monthly NASDAQ composite (0.41 (SD 0.67) v 0.5 (0.85), P=0.75), FTSE 100 index in cents (2.3 (6.8) v 3.1 (7.8), P=0.77), and PE ratio (0.54 (0.52) v 0.66 (0.29), P=0.50) did not change. The mean end-of-month closing value of the Dow Jones, however, decreased significantly (12 537 (841.4) v 8388 (699.8), P<0.001)There was no detectable difference in the total value of coins ingested, or ratio of coins to other objects swallowed, before or after a massive stock market crash.
Detection of EGFR gene mutations from daily obtained specimens in NSCLC patients
Authors: Tatsuro Fukuhara, Takuji Suzuki, Akira Inoue, Makoto Maemondo, Takuya Moriya, Hironobu Sasano, Takashi Kondo, Yasuo Saijo, Toshihiro Nukiwa
Journal: Cancer Research (2006)

4547   Purpose:Patients with non-small cell lung cancer (NSCLC) harboring activating somatic mutations of epidermal growth factor receptor (EGFR) are highly sensitive to the EGFR- tyrosine kinase inhibitor: gefitinib. We examined the detection of the EGFR gene mutation prospectively using a variety of daily specimens of NSCLC patients. The response to the gefitinib treatment was also evaluated. Patients and Methods: We prospectively evaluated 104 daily obtained specimens of 91 NSCLC Japnanese patients for EGFR mutations in exon 18-23. Isolated DNA were obtained from surgical direct or paraffin-embedded specimens, biopsied or cytology samples by fine needle biopsy or by transbronchoscopic biopsy, pleural effusions, and pericardial effusion. Results: Thirty-two mutations including deletions in exon 19 in 23 patients and L858R in 9 patients were detected. The frequency of the mutation were 47.2% (paraffin-embedded surgical lung specimens), 42.9% (fresh surgical or biopsied specimens), 38.1% (transbronchoscopic biopsied samples), 30.8% (transbronchoscopic cytology samples), 42.9% (biopsied samples from metastatic lesion) and 30.8% (pleural or pericardial effusion samples). Response rate of gefitinib treatment in patients with EGFR mutation was 65.0% (13 of 20 patients) compared to 37.5% (3 of 8 patients) in patients without mutation. Conclusion: Although lower than from surgical specimens, we could detect the EGFR mutations from daily available specimens. The detection of the EGFR gene mutations from diagnostic tumor specimens may make it possible to induce the first line gefitinib treatment.
Abstract P371: Prevalence of Cardiometabolically Healthy and Cardiometabolically Unhealthy Peruvians by Body Mass Index Categories: The CRONICAS Cohort Study
Authors: Catherine P Benziger, Antonio ` Bernabe-Ortiz, Robert H Gilman, William Checkley, Liam Smeeth, German Malaga, J. Jaime Miranda
Journal: Circulation (2015)

Background: Obesity is associated with a number of cardiometabolic risk factors. However, despite increased adiposity, some obese individuals display normal metabolic features. Conversely, individuals with normal weight can display metabolically unhealthy features. These profiles have not been researched in Latin American populations. We aimed to characterize cardiometabolic status by body mass index (BMI) status with emphasis on unhealthy cardiometabolic profile among normal weight individuals, and healthy cardiometabolic profile among overweight and obese. Methods: The CRONICAS Cohort is a an age- and sex-matched population-based study across four different geographical settings: Lima (Peru’s capital, urban), Tumbes (lowland, semirural), Puno rural and Puno urban (both high altitude). Individuals were classified into two groups: cardiometabolically healthy with (0-1 abnormality) or cardiometabolically unhealthy (≥2 abnormalities). The conditions for cardiometabolic assessment included components of the metabolic syndrome, high-sensitivity C-reactive protein, and insulin resistance. Results: A total of 3088 individuals, mean age 55.6 (SD±12.6) years, 51.3% females, had all measurements for this analysis. Of these, 889 subjects (28.8%), 1359 (44.1%) and 838 (27.1%) had normal weight, overweight and obese, respectively. Among normal weight individuals, 43.1% were cardiometabolically unhealthy (figure), and age ≥65 years, female, and highest wealth index groups were more likely to have this pattern. In contrast, only 16.0% of overweight and 3.6% of obese individuals were cardiometabolically healthy. In the combined overweight/obese group, compared to Lima, rural and urban sites in Puno were more likely of having the cardiometabolically healthier profile. Conclusions: Our results indicate a high prevalence of cardiometabolic abnormalities in Peru, specifically among normal weight individuals. Prevention programs to address cardiovascular risk need to include people of normal weight.
Growing Up Tobacco Free
Authors: Paul R. Torrens
Journal: JAMA (1995)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Identification of anti-renal cell carcinoma agents using pharmacological synthetic lethal screening
Authors: Haiyong Han, Yu Zhao, Aris E. Mosley, Daniel D. Von Hoff
Journal: Cancer Research (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

4699 

Renal cell carcinoma (RCC) is the most common malignant lesion of the kidney, comprising approximately 3% of all adult cancers. The primary treatment for clinically localized RCC is surgical removal of the lesion. There are some promising new approaches with VEGF/PDGF interactive agents. Remissions to these agents have been seen in a significant percentage of patients. One of the most significant advances in the past decade in our understanding of RCC is the identification of the von Hippel-Lindau disease (VHL) gene. The VHL gene is a tumor suppressor gene that has been found to be disabled either through mutations or through hypermethylation in over 75% of all RCC cases. Although it has been proven difficult for small molecule based cancer therapies to exclusively target a loss-of-function mutation like the VHL gene, recent advances in the drug development field provides new prospective for such strategy. In this study, we used a pharmacological synthetic lethal screening approach (Wang et al, Proc. AACR, 45:1421, 2004) to identify new agents that target specifically renal cancer cells with VHL deficiency. To do so, a pair of isogenic cell lines (786-O-vector and 786-O-VHL) for the VHL gene were generated and used to screen chemical libraries using the standard MTS-based cell proliferation assay. After screening about 10,000 compounds (the NCI diversity set and a subset of the NanoSyn Pharm library), we identified 3 potential leads that showed greater than 2 fold selectivity against the renal cancer cell line with inactivated VHL gene (786-O-vector), compared to the cell line with wildtype VHL (786-O-VHL). The IC50s of the compounds in the VHL deficient cell line range from low nanomolar to low micromolar. The selectivity of the compounds was further confirmed in a second pair of VHL isogenic cell lines. We are currently characterizing the potential mechanism of action of these compounds using flow cytometry and apoptosis assays. Further development of these leads can potentially lead to new therapies for the treatment of patients with RCC. (This work was supported by a grant from the Arizona Biomedical Research Commission)
On Being Human
Authors: N A Dimeo
Journal: JAMA (1998)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Acute loss of lung function without wheezing during bee venom immunotherapy
Authors: A. M. Chiriac, F. Gouzi, J. Bousquet, P. Demoly
Journal: Allergy (2011)

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not.The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Acute loss of lung function without wheezing during bee venom immunotherapy