tbuckley/cabot-search huggingface dataset:
Pollen proteases compromise the airway epithelial barrier through degradation of transmembrane adhesion proteins and lung bioactive peptides
Authors: R. Vinhas, L. Cortes, I. Cardoso, V. M. Mendes, B. Manadas, A. Todo-Bom, E. Pires, P. Veríssimo
Journal: Allergy (2011)

To cite this article: Vinhas R, Cortes L, Cardoso I, Mendes VM, Manadas B, Todo-Bom A, Pires E, Veríssimo P. Pollen proteases compromise the airway epithelial barrier through degradation of transmembrane adhesion proteins and lung bioactive peptides. Allergy 2011; 66: 1088–1098. Background: Allergic disorders, such as seasonal rhinitis and asthma, are increasing causes of morbidity worldwide and often result from exposure to airborne pollen. Pollen allergy has a remarkable clinical impact all over Europe. In fact, epidemiological longitudinal studies confirm that pollen species usually considered with low allergenic potential became more recently responsible for intense allergic reactions. In this study, we aimed to characterize major pollen proteolytic activity and evaluate its contribution to the immunologic and inflammatory response to airborne allergens. Methods: Proteolytic activity in four pollen diffusates with distinct allergenicity, Olea europaea, Dactylis glomerata, Cupressus sempervirens and Pinus sylvestris, was evaluated through several enzymatic assays. The action of pollen proteases on the paracellular integrity of Calu-3, grown at the air–liquid interphase, was evaluated through a transepithelial permeability assay. Immunoblot and immunofluorescence experiments were performed to analyse the disruption of intercellular complexes. Degradation of bioactive peptides by pollen crude extracts was assessed by mass spectrometry. Results: All pollen diffusates were shown to have high molecular weight proteases with serine and/or aminopeptidase activity. These proteases increased Calu-3 transepithelial permeability through disruption of transmembrane adhesion proteins: occludin, claudin-1 and E-cadherin. Moreover, they were able to degrade airway bioactive peptides and were not blocked by endogenous protease inhibitors. Conclusion: Pollen grains with distinct allergenic abilities release proteases that might be involved in the sensitization to a range of airborne allergens by facilitating allergen delivery across the epithelium and also contribute directly to the inflammation characteristic of allergic diseases.
Abstract P070: A Low Carbohydrate Diet from Plant or Animal Sources and Mortality Among Myocardial Infarction Survivors
Authors: Shanshan Li, Alan Flint, Jennifer Pai, John P Forman, Frank B Hu, Walter C Willett, Kathryn M Rexrode, Kenneth J Mukamal, Eric B Rimm
Journal: Circulation (2014)

Background: The healthiest dietary pattern for myocardial infarction (MI) survivors is not known. Specific long-term benefits of a low carbohydrate diet (LCD) is unknown, whether it mainly be from animal or vegetable sources. Objective: To examine the associations between post-MI adherence to a low carbohydrate diet (LCD), measured by a total, plant- and animal-based low carbohydrate diet score (LCDS), in relation to all-cause and cardiovascular mortality. Design: We included 2,258 women from the Nurses’ Health Study and 1,840 men from the Health Professional Follow-Up Study, who had survived a first MI during follow-up, provided pre-MI and at least one post-MI food frequency questionnaire (FFQ). Results: Adherence to a LCD high in animal sources of protein and fat was associated with higher all-cause and cardiovascular mortality (HR=1.31, 95% CI: 1.05-1.63 for all-cause mortality; HR= 1.49, 95% CI: 1.08-2.06 for cardiovascular mortality comparing extreme quintiles). An increase in adherence to an animal-based LCD prospectively assessed from the pre- to post-MI period was associated with higher all-cause mortality and cardiovascular mortality (HR=1.29, 95% CI: 1.03-1.63 for all-cause mortality; HR=1.53, 95% CI: 1.10-2.12 for cardiovascular mortality comparing extreme quintiles). An increase in adherence to a plant-based LCD was not associated with lower all-cause or cardiovascular mortality. Conclusions: Greater adherence to a LCD high in animal sources of fat and protein was associated with higher all-cause and cardiovascular mortality post-MI. We did not find a health benefit from greater adherence to an overall LCD post MI.
Donor NK cell licensing in control of malignancy in hematopoietic stem cell transplant recipients
Authors: Jacek Nowak, Katarzyna Kościńska, Renata Mika‐Witkowska, Marta Rogatko‐Koroś, Sylwia Mizia, Emilia Jaskuła, Małgorzata Polak, Monika Mordak‐Domagała, Janusz Lange, Anna Gronkowska, Wiesław Wiktor Jędrzejczak, Sławomira Kyrcz‐Krzemień, Mirosław Markiewicz, Monika Dzierżak‐Mietła, Agnieszka Tomaszewska, Barbara Nasiłowska‐Adamska, Andrzej Szczepiński, Kazimierz Hałaburda, Andrzej Hellmann, Mieczysław Komarnicki, Lidia Gil, Anna Czyż, Jacek Wachowiak, Małgorzata Barańska, Jerzy Kowalczyk, Katarzyna Drabko, Jolanta Goździk, Barbara Wysoczańska, Katarzyna Bogunia‐Kubik, Elżbieta Graczyk‐Pol, Agnieszka Witkowska, Anna Marosz‐Rudnicka, Klaudia Nestorowicz, Joanna Dziopa, Urszula Szlendak, Krzysztof Warzocha, and Andrzej Lange
Journal: American Journal of Hematology (2014)

Among cancers treated with allogeneic hematopoietic stem-cell transplantation (HSCT), some are sensitive to natural killer (NK) cell reactivity, described as the "missing self" recognition effect. However, this model disregarded the NK cell licensing effect, which highly increases the NK cell reactivity against tumor and is dependent on the coexpression of inhibitory killer cell immunoglobulin-like receptor (iKIR) and its corresponding HLA Class I ligand. We assessed clinical data, HLA and donor iKIR genotyping in 283 patients with myelo- and lymphoproliferative malignancies who underwent HSCT from unrelated donors. We found dramatically reduced overall survival (OS), progression free survival (PFS), and time to progression (TTP) among patients with malignant diseases with the lack of HLA ligand cognate with this iKIR involved in NK cell licensing in corresponding donor (events 83.3% vs. 39.8%, P = 0.0010; 91.6% vs. 47.7%, P = 0.00010; and 30.0% vs. 17.3%, P = 0.013, for OS, PFS, and TTP, respectively). The extremely adverse PFS have withstand the correction when patient group was restricted to HLA mismatched donor-recipient pairs. The incidence of aGvHD was comparable in two groups of patients. In malignant patients after HSCT the missing HLA ligand for iKIR involved in NK cell licensing in corresponding donor ("missing licensing proof") induced extremely adverse survival of the patients due to the progression of malignancy and not to the aGvHD. Avoiding the selection of HSCT donors with the "missing licensing proof" in the malignant patient is strongly advisable.
İleri Evre Küçük Hücreli Dışı Akciğer Kanserinin Tedavisinde Birinci Sıra Sistemik Kemoterapi
Authors: John Goffin, Christina Lacchetti, MHSc, Peter M. Ellis, Yee C. Ung, William K. Evans
Journal: Journal of Thoracic Oncology (2010)
MITRAL STENOSIS COMPLICATING SITUS INVERSUS: A CASE SUCCESSFULLY TREATED BY COMMISSUROTOMY
Authors: D BERKOWITZ, W LIKOFF
Journal: Annals of Internal Medicine (1954)

Case Reports1 April 1954MITRAL STENOSIS COMPLICATING SITUS INVERSUS: A CASE SUCCESSFULLY TREATED BY COMMISSUROTOMYDONALD BERKOWITZ, M.D., WILLIAM LIKOFF, M.D., F.A.C.P.DONALD BERKOWITZ, M.D.Search for more papers by this author, WILLIAM LIKOFF, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-40-4-784 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptMitral stenosis complicating situs inversus is an uncommon finding. Since we have been able to find only one other case described in the literature,1 we believe it of sufficient interest to report such a patient successfully treated by mitral commissurotomy.CASE REPORTA 32 year old white housewife was admitted to the Thoracic Surgical Department of Hahnemann Hospital on November 17, 1952. There was no history of rheumatic fever during childhood or thereafter. At the age of 26, during the third trimester of her first pregnancy, the patient developed nocturnal dyspnea with hemoptysis, but nevertheless went on to a normal...Bibliography1. Owen SA: A case of complete transposition of the viscera associated with mitral stenosis, Heart 3: 113, 1911. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Philadelphia, Pennsylvania*Received for publication August 1, 1953.From the Departments of Medicine and Thoracic Surgery, Hahnemann Medical College and Hospital. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byClosed mitral commissurotomy for mitral stenosis with dextrocardia—case seriesSpecifics of technique in percutaneous mitral commissurotomy in a case of dextrocardia and situs inversus with mitral stenosisMITRAL VALVOTOMY IN SITUS INVERSUS WITH ASSOCIATED SKELETAL ANOMALIESRHEUMATIC MITRAL STENOSIS AND AORTIC INCOMPETENCE IN A CASE OF DEXTROCARDIA WITH SITUS INVERSUS 1 April 1954Volume 40, Issue 4Page: 784-788KeywordsDyspneaHospital medicineMitral stenosisPregnancyRheumatic feverThoracic surgery ePublished: 1 December 2008 Issue Published: 1 April 1954 PDF downloadLoading ...
Trbp regulates heart function through microRNA-mediated Sox6 repression
Authors: Jian Ding, Jinghai Chen, Yanqun Wang, Masaharu Kataoka, Lixin Ma, Pingzhu Zhou, Xiaoyun Hu, Zhiqiang Lin, Mao Nie, Zhong-Liang Deng, William T Pu, Da-Zhi Wang
Journal: Nature Genetics (2015)
Myocardial strain measurements are not equivalent across techniques: A validation of two-dimensional and tissue velocity based strain with harmonic phase magnetic resonance imaging
Authors: Manish Bansal, Goo-Yeong Cho, Jonathan Chan, Rodel Leano, Brian A. Haluska, Thomas H. Marwick
Journal: Circulation (2007)

Background: The measurement of myocardial deformation offers a means of quantifying regional LV function. Conventional tissue velocity (TVI) based strain suffers the problem of angle dependence, and 2D echocardiography based strain measurements have been developed to overcome this limitation. The aim of this study was to compare two such techniques (2DS1 & 2DS2) with TVI-strain, using harmonic phase magnetic resonance imaging (MRI, which offers excellent image quality) as the reference standard. Methods: Thirty pts (mean age 62±11yrs) with known or suspected ischemic heart disease were included in the study. Peak-systolic longitudinal (eL) and radial strain (eR) were measured in 16 segments from the three conventional apical views using 2DS1, 2DS2, TVI (only eL) and MRI. Segments were also categorized as normal (n=247) or abnormal (n=102) based on gadolinium-contrast enhancement on MRI.Results: Although there was significant correlation between the echo and MRI measures of eL, all three echo techniques underestimated eL compared to MRI (Table). eL2DS2 was significantly higher than eL2DS1 (p=0.002) and had the best correlation with eLMRI. The correlation between eL2DS2 and eLMRI was better for abnormal than normal segments (p = <0.001). On ROC curve analysis, eL2DS2 was found to have the greatest AUC (0.84) compared to eL2DS1 (0.75; p= <0.001) or eLTVI (0.76; p= <0.001) for identification of dysfunctional segments. In case of radial strain, only eR2DS2 showed significant correlation with eRMRI.Conclusions: Different 2D echocardiographic techniques provide significantly different measures of myocardial strain and the discrepancy is greater for radial strain than the longitudinal strain. These findings imply that strain measurement techniques cannot be used interchangeably.
Abstract 13597: The Association Between Tissue Morphology Assessed With Optical Coherence Tomography and Mid-term Results After Paclitaxel-coated Balloon Dilatation
Authors: Takeshi Tada, Kazushige Kadota, Seiji Habara, Hiroyuki Tanaka, Yasushi Fuku, Tsuyoshi Goto, Kazuaki Mitsudo
Journal: Circulation (2014)

Introduction: It was reported that mid-term results after paclitaxel-coated balloon (PCB) dilatation for in-stent restenosis (ISR) lesions might differ depending on the tissue morphology of ISR lesions assessed with optical coherence tomography (OCT). It was also reported that ISR lesions after drug-eluting stent (DES) implantation was associated with poorer mid-term outcomes compared with those after bare-metal stent (BMS) implantation after PCB dilatation. The aim of this study was to define the impact of OCT findings on recurrence of ISR after PCB dilatation. Methods: Between October 2008 and December 2013, we performed percutaneous coronary intervention for 210 ISR lesions in 180 patients using PCB (143 men, mean age 68.8±9.4 years). The restenotic tissue structure at the minimum lumen area site (homogeneous, heterogeneous, or layered type) was assessed with OCT. We examined the association between tissue structure and acute/mid-term (6-8 months) results including acute gain, late loss, ISR and target lesion revascularization rates. Furthermore, we also examined the distribution of tissue structure types in each previous procedure type including BMS/DES implantation and PCB dilatation. Results: The mean follow-up period was 194±36 days. The association of tissue structure with mid-term results is shown in the figure. The late loss was significantly larger in lesions with heterogeneous structure than in those with homogeneous and layered structures. The prevalence of heterogeneous structure in each previous procedure type is also shown in the figure. The prevalence of heterogeneous structure was significantly lower in lesions after BMS implantation than in those after PCB dilatation. Conclusions: The tissue morphology of ISR lesion assessed with OCT may have an impact on the mid-term efficacy of PCB. The difference in distribution of tissue structure types may also be related to the difference between efficacies of PCB with each previous procedure type.
How many conditions can a GP screen for?
Authors: C. Del Mar
Journal: BMJ (2003)

Evidence based information on diagnostic tests will help 

How many general practitioners will read this week's paper by Arroll et al on diagnosing depression (p 1144)1 and sigh, “Another thing we have to do”? On the face of it we have a simple and accurate “test” for depression that is effective. Does this then impose a burden on the backs of general practitioners, another duty that will attract censure if not done properly? We think not. Rather, we ask what can be dropped, and how we can simplify our clinical work?

Making diagnoses has often been taught as a long and complicated process. “First take a thorough history” is the unhelpful advice from textbooks. What does that mean? Ask what is often a muddle of questions, and listen to a lot of information. Then pursue some lines of inquiry …
Abstract 12364: Circulating Cardiac and Inflammatory Biomarkers to Predict Post-Operative Atrial Fibrillation in the OPERA Trial
Authors: Serge Masson, Jason H Wu, Simona Barlera, Laura Bani, Roberto Marchioli, Alejandro Macchia, Tarcisio Vago, John D Puskas, Roberto René Favaloro, Alejandro Rubén Hershson, Riccardo Sinatra, Giovanni Melina, Liliana Nicolosi, Lorella Dreas, John VanderWoude, Gianni Tognoni, Darisuh Mozaffarian, Roberto Latini
Journal: Circulation (2014)

Objectives: Post-operative atrial fibrillation (POAF) is a common complication after cardiac surgery and predicts increased morbidity and mortality. Identification of patients at high risk of POAF with the help of circulating biomarkers may enable early preventive treatment but data are limited, especially in contemporary surgical patients. Methods: Plasma concentrations of N-terminal probrain natriuretic peptide (NT-proBNP), high sensitivity cardiac troponin T (hs-cTnT) and C-reactive protein (hsCRP) were measured at enrollment, on the morning of cardiac surgery, at end-surgery, and 2 days post-surgery in 562 patients undergoing cardiac surgery, randomized to perioperative supplementation with oral fish oil or placebo in the international clinical trial Omega-3 Fatty Acids for Prevention of Post-Operative Atrial Fibrillation trial (OPERA). The primary endpoint was incident POAF lasting ≥ 30 seconds, centrally adjudicated and confirmed electrocardiographically. Results: Higher levels of NT-proBNP and hs-cTnT before surgery were associated with older age, renal or cardiac dysfunction and higher EuroSCORE, while hsCRP correlated positively with BMI. NT-proBNP (2172 [1238-3758] ng/L) and hsCRP (118 [75-165] mg/L) peaked on post-operative day 2, while hs-cTnT peaked at the end of surgery (373 [188-660] ng/L). Fish oil supplementation did not alter the time course of the cardiac or inflammatory biomarkers. Concentrations of the 3 biomarkers, on the morning of surgery, or changes in their level between morning of surgery and post-surgery, were not significantly associated with POAF after adjustment for clinical and surgical characteristics. Conclusions: Among patients undergoing cardiac surgery, cardiac markers are related to clinical and surgical characteristics, have different perioperative time courses but do not help in predicting the risk of POAF.
A 16-gene assay to predict recurrence after surgery in localised renal cell carcinoma: development and validation studies
Authors: Brian Rini, Audrey Goddard, Dejan Knezevic, Tara Maddala, Ming Zhou, Hakan Aydin, Steven Campbell, Paul Elson, Serge Koscielny, Margarita Lopatin, Christer Svedman, Jean-Francois Martini, J Andrew Williams, Virginie Verkarre, Camelia Radulescu, Yann Neuzillet, Isabelle Hemmerlé, Marc Olivier Timsit, Athanasios C Tsiatis, Michael Bonham, Thierry Lebret, Arnaud Mejean, Bernard Escudier
Journal: The Lancet Oncology (2015)
Sustained Enhancement of Cardiac Function Follows Increased Recruitment of Pro-angiogenic Macrophages to Healing Infarcts of 11 Beta Hydroxysteroid Dehydrogenase Type 1 Deficient Mice
Authors: Sara J. McSweeney, Agnieszka M. Kozak, Hiba Khaled, Patrick W. Hadoke, Brian R. Walker, Gillian A. Gray
Journal: Circulation (2009)
Abstract 593: Rb association with Bax is regulated by phosphorylation
Authors: Lisa Antonucci, Jacklynn V. Egger, Nancy A. Krucher
Journal: Cancer Research (2014)

Abstract The Retinoblastoma protein (Rb) is important in the control of cell proliferation and apoptosis. Its activity is controlled by reversible phosphorylation on several serine and threonine residues that mediate binding to various cellular proteins. The role of Rb phosphorylation in the control of apoptosis is largely unknown, although several apoptotic stimuli result in dephosphorylation of Rb. Our previous work identified Rb (threonine-821) dephosphorylation as required for induction of apoptosis caused by UV light and inhibitions of cyclin dependent kinases. These studies led to the identification of proteins that bind to Rb under apoptotic conditions. One such Rb binding protein is the pro-apoptotic protein Bax. Bax, a member of the Bcl-2 family, undergoes a conformational change during apoptosis that is associated with the permeabilization of the outer mitochondrial membrane. In this study, we examined the role of Rb phosphorylation in the regulation of binding to Bax. In MCF7 cells, by activating the phosphatase responsible for dephosphorylating Rb, PP1, apoptotic death is triggered. We utilize siRNA to block the expression of PNUTS (Phosphatase Nuclear Targeting Subunit) which causes Rb dephosphorylation, and cell death. We have found that Bax binds to Rb under normal cell conditions, but when Rb becomes dephosphorylated on specific amino acid sites, Bax dissociates from Rb. Our cell fractionation studies have shown that phosphorylated Rb co-localizes with the mitochondrial fraction. Further examination of the specific sites of Rb phosphorylation required for binding to Bax is underway. Citation Format: Lisa Antonucci, Jacklynn V. Egger, Nancy A. Krucher. Rb association with Bax is regulated by phosphorylation. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 593. doi:10.1158/1538-7445.AM2014-593
Swanzy's Handbook of the Diseases of the Eye and Their Treatment.
Authors: Henry Rosborough Swanzy, Louis Werner
Journal: JAMA (1920)

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PLA2G2A, a novel β-catenin/TCF target gene, inhibits gastric cancer invasion and metastasis
Authors: Kumaresan Ganesan, Tatiana Ivanova, Yongui Wu, Jeanie Wu, Yu Kun, Ming Hui, Vikneswari Rajasegaran, Heike Grabsch, Gerrit Meijer, Suet Leung, Oi Kon, Patrick Tan
Journal: Clinical Cancer Research (2007)

B62   Carcinoma of the stomach (gastric cancer) is the second highest cause of worldwide cancer mortality. Expression of  PLA2G2A , a secreted phospholipase, in gastric tumors has previously been shown to carry prognostic relevance, but the pathways regulating  PLA2G2A , and how it mediates its pro-survival effect, are currently unknown. In this report, we demonstrate that  PLA2G2A  is a direct target of the Wnt signaling pathway, whose expression is reduced in late stage and metastatic gastric, colon, and prostate tumors compared to early stage carcinomas. We found evidence of  PLA2G2A  genomic deletion and promoter methylation in gastric cancer cell lines, and that  PLA2G2A  can inhibit the invasive ability of gastric cancer cells through the transcriptional down-regulation of metastasis mediator genes such as  S100A4  and  NEDD9 . Our results thus shed light on  PLA2G2A’s  mechanism of action in gastric cancer, and suggest that the induced expression of  PLA2G2A , either by exogenous administration or endogenous reactivation, may represent a novel therapeutic approach to treat late stage and metastatic gastric cancers.