tbuckley/cabot-search huggingface dataset:
Abstract P243: Greater Healthful Dietary Variety and Changes in Adiposity Indicators During a Weight-Loss Intervention: The POUNDS Lost Trial
Authors: Maya Vadiveloo, Frank M Sacks, George A Bray, Catherine Champagne, Josiemer Mattei
Journal: Circulation (2015)

Background: Adhering to a healthy lower energy diet is essential for sustained weight loss. Greater variety within energy-poor, nutrient-dense foods may improve adherence by making the diet less monotonous, and therefore more enjoyable. Greater healthful dietary variety has been inversely associated with adiposity in cross-sectional studies, but it is unclear if it predicts longitudinal changes in body weight. Objective: To examine the longitudinal associations between initial changes in healthful dietary variety (0-6 months) and long-term changes in adiposity over 24-months in a weight-loss trial. Methods: Participants in the POUNDS Lost trial were advised to follow energy-restricted diets. Data for participants with complete dietary assessments at baseline and 6-months were analyzed (n=367). Healthful dietary variety was quantified using the validated US Healthy Food Diversity (US HFD) index, which measures the number of foods consumed and consistency of dietary selections and amounts with recommendations from the 2010 Dietary Guidelines for Americans. Changes in the US HFD index between baseline and 6-months were divided into tertiles (T); adults in T1 had reductions in the US HFD index, T2 maintained stable index values, and T3 had increased index values. Body weight and waist circumference (WC) were measured at 6, 12, 18, and 24-months and percent body fat and trunk fat were measured at 6 and 24-months using DEXA. Repeated measures ANOVA was used to test whether US HFD index change tertiles were significantly associated with adiposity over 24-months. Models were adjusted for age, sex, race, smoking, education, income, diet arm, changes in physical activity and energy intake, baseline adiposity indicator, and the interaction between the US HFD index change tertile and time. Results: Reductions in energy intake were equivalent across US HFD index change tertiles in multivariable models (-425 vs.-265 kcal in T1 vs.T3, p=0.08). An improved variety (T3) as compared to stable variety (T2) was associated with greater weight loss at 24-months (-4.75 vs. -2.63kg, p=0.03; linear p-trend=0.04) and greater decrease in total body fat in T3 vs. T2 (-1.10% vs. -0.006%, p=0.03; p-trend=0.04) and trunk fat (-1.29% vs. 0.08%, p=0.03; p-trend=0.04). An improved variety (T3) as compared to stable variety (T2) or reduced variety (T1) was associated with greater reduction in WC (-6.48cm (T3) vs. -3.98cm (T2), p=0.01; and -4.14cm (T1), p=0.04; linear p-trend=0.01). Reducing variety (T1) did not reduce or increase other adiposity indicators compared to stable or increased variety. Conclusions: Increasing healthful dietary variety while restricting energy intake may improve sustained reductions in adiposity among adults trying to lose weight.
Three cases of carbamazepine toxicity
Authors: N C Moore, B Lerer, E Meyendorff, S Gershon
Journal: American Journal of Psychiatry (1985)

The growing number of psychiatrists who prescribe carbamazepine therapy should be alert to the drug's potential complications. The authors report three such cases: one of hepatitis, one of a fatality due to aplastic anemia, and one of a severe dermatological reaction.
Abstract P029: Sucralose Promotes Increase in Fat Accumulation in Human Mesenchymal Stem Cells
Authors: Sabyasachi Sen, Carol Rouphael, Sara Houston
Journal: Circulation (2015)

Background: Artificial sweeteners are extensively used nowadays as a non-caloric sugar alternative. They are sweeter than sugar, with a presumed high safety profile, hence commonly promoted in weight loss and weight maintenance programs as a sugar substitute. However, recent studies showed that saccharin and acesulfame potassium may actually increase adipogenesis. Here we choose to study the effect of Sucralose on Mesenchymal Stem Cells (MSCs), which was never been tested before. MSCs are multipotent cells which can differentiate to adipocytes, myoblasts, osteoblasts or chondroblasts. We were interested to note if presence of sucralose promotes adipocyte formation. Methods: We cultured MSCs in Normal Glucose DMEM media (5.5mM) or in Adipogenic Media (Lonza Inc., 5.5mM) with or without 0.00mM, 0.45mM or 4.5mM of Sucralose for a total of 6 days. At the end of day 6, cells were stained with Oil Red O stain (lipolysis). Cells were lysed post staining and absorbance of the assimilated dye was measured using a plate reader at 520 nm. Non-stained cells from each media and Sucralose concentration were also lysed as control and RNA was collected for RT-PCR to measure oxidation, inflammation and adipogenesis gene expression estimation. Results: Before lysing the cells for absorbance readings, cells were observed under the microscope for phase contrast image. As we increased the Sucralose concentration, (10-fold) a higher number of fat droplets was observed in MSCs cultured. Moreover, absorbance measurements showed that adipogenesis increased by 1.8 folds as we increased the Sucralose concentration from 0.00mM to 0.45mM. It was also increased by 2.85 folds as we increased the concentrations from 0.00mM to 4.5mM in cells cultured in both Normal Glucose and Adipogenic Media. RT-PCR results for oxidation genes ( Catalase, Superoxide dismutase 1, 2 and 3) , inflammation (TNF, IL-6) and adipogenesis( leptin, adiponectin, PPAR-g, FABP-4, c/EBP alpha and c/EBP beta) are pending. In summary, sucralose appears to promote fat accumulation by Oil Red O stain quantification. RT-PCR studies will confirm if adipogenic genes follow a similar pattern of up-regulation. Conclusion: Our studies indicate that sucralose may promote fat accumulation and warrants further cellular and animal model studies
Abstract 19052: Mitochondrial Spatial and Temporal Resolution Before and After Left Ventricular Assist Device Support in Advanced Heart Failure Patients
Authors: David A Liem, Jan Mulder, Marie Skogs, Shaun Setty, Martin Cadeiras, Jun Zhang, X’avia Chan, Amanda Lin, Caitlin Black, Erik Larsson, Anna Asplund, Emma Lundberg, Fredrik Ponten, James Weiss, Mario Deng, Jacob Odeberg, Mathias Uhlén, Peipei Ping
Journal: Circulation (2014)

Background: Advanced heart failure (HF) is associated with structural and functional perturbations in mitochondria, indicating an impaired oxidative phosphorylation. Left ventricular assist device (LVAD) support in HF patients has demonstrated significant recovery of cardiac function and molecular cellular defects. However, comprehensive imaging technologies, demonstrating the spatial and temporal proximities of organellar molecules that improve diagnostic HF stratification in parallel with conventional clinical markers, are still lacking. Aims: The aim of this study is to explore the recovery of mitochondrial function and molecular structure in advanced HF after LVAD implantation through high-resolution confocal microscopic immunofluorescence (IF) in conjunction with conventional biochemical assays. Methods: Fresh LV biopsies from 6 patients were obtained during LVAD implantation (PRE-VAD) and during their consecutive heart transplantation (POST-VAD); mitochondria were subsequently isolated. Results: Con...
Challenges Facing Family Practice and Primary Care
Authors: Weldon C. Flint
Journal: JAMA (2003)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 5466: The Mdm2 inhibitor, NVP-CGM097, in combination with the BRAF inhibitor NVP-LGX818 elicits synergistic antitumor effects in melanoma
Authors: Hui Qin Wang, Matthew Zubrowski, Erling Emerson, Elina Pradhan, Sébastien Jeay, Marion Wiesmann, Giordano Caponigro, Jens Wuerthner, Robert Schlegel, Z. Alexander Cao, Alan Huang, Ensar Halilovic
Journal: Cancer Research (2014)

Abstract RAF kinase inhibitors have shown substantial therapeutic effects in patients with BRAF-mutant melanoma. However, despite impressive initial responses, therapeutic effects are often temporary due to acquired resistance. This resistance highlights the need to identify therapeutic approaches to improve the durability of responses to RAF inhibitors. In this study, we demonstrate that a novel inhibitor of p53-Mdm2 interaction, NVP-CGM097, causes marked reduction of melanoma cell viability. The activation of p53 signaling and consequent reduction in cell viability required wild-type p53, but was independent of BRAF status. Moreover, combined inhibition of Mdm2, using NVP-CGM097, and BRAF using NVP-LGX818, synergistically inhibited the viability of BRAF mutant melanoma cells in vitro and tumor growth in vivo. NVP-CGM097 caused induction of p21 and Bax, while NVP-LGX818 did not. Instead, NVP-LGX818 caused induction of p27 and Bim. Therefore, together they induced a complementary set of anti-proliferative and apoptosis stimulating molecules, which resulted in strong synergistic antitumor effects. In vivo, the combination treatment resulted in sustained tumor regressions and markedly prolonged survival relative to either single agent. Collectively, these data show that activation of p53, through inhibition of Mdm2, leads to antitumor effects in melanoma. Furthermore, the data show that concomitant activation of tumor suppressor p53 and inhibition of BRAF synergistically suppresses melanoma growth. Therefore, combined inhibition of Mdm2 and BRAF in melanoma may provide an effective therapeutic modality capable of overcoming the resistance observed with the BRAF inhibitor monotherapy and thus lead to more durable responses in the clinic. Citation Format: Hui Qin Wang, Matthew Zubrowski, Erling Emerson, Elina Pradhan, Sébastien Jeay, Marion Wiesmann, Giordano Caponigro, Jens Wuerthner, Robert Schlegel, Z. Alexander Cao, Alan Huang, Ensar Halilovic. The Mdm2 inhibitor, NVP-CGM097, in combination with the BRAF inhibitor NVP-LGX818 elicits synergistic antitumor effects in melanoma. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5466. doi:10.1158/1538-7445.AM2014-5466
Potential vectors and hosts of rickettsia spp: epidemiological studies in the Vale do Paraíba, state of Rio de Janeiro/Brazil
Authors: G.S. Gazeta, E.R. Souza, A.E. Abboud-Dutra, M. Amorim, P.R. Barbosa, A.B. Almeida, V. Gomes, F.S. Gehrke, M.T. Marrelli, T.T.S Schumaker
Journal: Clinical Microbiology and Infection (2009)
THE GREAT NEED FOR INTERNISTS IN THE NAVAL MEDICAL PROGRAM
Authors: Ross T. Mcintire
Journal: Annals of Internal Medicine (1944)

Article1 July 1944THE GREAT NEED FOR INTERNISTS IN THE NAVAL MEDICAL PROGRAMROSS T. MCINTIREROSS T. MCINTIRESearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-21-1-1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptOur outstanding President, Dr. Paullin, has given so much to the war effort that I want to take this opportunity to express to him the thanks and appreciation of the Navy for the splendid work he has done. In addition to his being President of our College, and President of the American Medical Association, he has assumed an added responsibility as Honorary Consultant to the Secretary of the Navy and the Surgeon General of the Navy. I can assure you that as he relinquishes the details of the two positions which he now holds in civil medicine, we in the... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Medical Corps U.S.N. The Surgeon General of the Navy*Presented by Rear Admiral Luther Sheldon, Jr., Medical Corps, U.S.N., at the Scientific War Session, American College of Physicians, Palmer House, Chicago, Illinois, April 1, 1944. Nextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 July 1944Volume 21, Issue 1Page: 1-6KeywordsSurgeons ePublished: 1 December 2008 Issue Published: 1 July 1944 Copyright & PermissionsCopyright, 1944, by The American College of PhysiciansPDF downloadLoading ...
Promoting Balance in the Lives of Resident Physicians
Authors: Arghavan Salles, Cara A. Liebert, Ralph S. Greco
Journal: JAMA Surgery (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal
Impact of Routine Angiographic Follow-Up on the Clinical Benefits of Paclitaxel-Eluting Stents
Authors: Duane S. Pinto, Gregg W. Stone, Stephen G. Ellis, David A. Cox, James Hermiller, Charles O’Shaughnessy, J. Tift Mann, Roxana Mehran, Yingbo Na, Mark Turco, Ronald Caputo, Jeffrey J. Popma, Donald E. Cutlip, Mary E. Russell, David J. Cohen
Journal: Journal of the American College of Cardiology (2006)
The RADAR Project and the FDA
Authors: Anne E Trontell
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 13960: Global Two-Dimensional Strain as a Robust Parameter of Myocardial Fibrosis in Patients With Hypertrophic Cardiomyopathy
Authors: Makoto Saito, Yasuhiro Sasaki, Hiroe Morioka, Toyofumi Yoshii, Go Hiasa, Takumi Sumimoto, Kazuhisa Nishimura, Katsuji Inoue, Hideki Okayama, Jitsuo Higaki
Journal: Circulation (2010)

Background: Myocardial fibrosis can lead to an increase in chamber stiffness, Resultsing in left ventricular (LV) systolic and diastolic dysfunction. Late gadolinium enhancement (LGE) on contrast-e...
Prostaglandin Production in Cultures of BALB/3T3 and SV3T3 Mouse Fibroblasts 2
Authors: Earl M. Ritzi, William A. Stylos
Journal: JNCI Journal of the National Cancer Institute (1976)

The concentration of different prostaglandins (PG's) was determined in both cells and culture medium of growing BALB/3T3 and BALB/3T3 (3T3) cultures transformed by simian virus 40 (SV3T3). Most PG's were found in the culture medium rather than in the cells. Further, the larger PG measurements were PGE and a composite measurement of PGA and PGB. PGF was detected at lower levels. The sum of PGE and the composite measurement (PGA+PGB) was the best indication of PG production in culture. When 2-day medium collections from 3T3 and SV3T3 cells were measured by radioimmunoassay for PGs, higher concentrations of PG were detected in the media of SV3T3 cultures. This difference in PG production was not due merely to differences in cell density, since SV3T3 cells produced higher PG concentrations, even at equal cell densities. PG production for a 2-day interval was more a function of cell type than cell density.
Qualitative Research: Rigour and qualitative research
Authors: N. Mays, C. Pope
Journal: BMJ (1995)

Various strategies are available within qualitative research to protect against bias and enhance the reliability of findings. This paper gives examples of the principal approaches and summarises them into a methodological checklist to help readers of reports of qualitative projects to assess the quality of the research.

In the health field--with its strong tradition of biomedical research using conventional, quantitative, and often experimental methods--qualitative research is often criticised for lacking scientific rigour. To label an approach “unscientific” is peculiarly damning in an era when scientific knowledge is generally regarded as the highest form of knowing. The most commonly heard criticisms are, firstly, that qualitative research is merely an assembly of anecdote and personal impressions, strongly subject to researcher bias; secondly, it is argued that qualiative research lacks reproducibility--the research is so personal to the researcher that there is no guarantee that a different researcher would not come to radically different conclusions; and, finally, qualitative research is criticised for lacking generalisability. It is said that qualitative methods tend to generate large amounts of detailed information about a small number of settings.

The pervasive assumption underlying all these criticisms is that quantitative and qualitative approaches are fundamentally different in their ability to ensure the validity and reliability of their findings. This distinction, however, is more one of degree than of type. The problem of the relation of a piece of research to some presumed underlying “truth” applies to the conduct of any form of social research. “One of the greatest methodological fallacies of the last half century in social research is the belief that science is a particular set of techniques; it is, rather, a state of mind, or attitude, and the organisational conditions which allow that attitude to be expressed.”1 In quantitative data analysis it is possible to generate statistical …
Gut Cryptopatches
Authors: Kenji Suzuki, Takatoku Oida, Hiromasa Hamada, Osamu Hitotsumatsu, Mamoru Watanabe, Toshifumi Hibi, Hiroshi Yamamoto, Eiro Kubota, Shuichi Kaminogawa, Hiromichi Ishikawa
Journal: Immunity (2000)
Abstract 11948: Dominant Negative 14-3-3 Protein Exacerbates Cardiac Dysfunction by Suppressing Cardiac Regeneration After Coronary Artery Ligation in Mice
Authors: Rajarajan Amirthalingam Thandavarayan, Somasundaram Arumugam, Vigneshwaran Pitchaimani, Vengadeshprabhu Karuppa Gounder, Vijayasree V Giridharan, Remya Sreedhar, Meilei Harima, Darukeshwara Joladarashi, Kenji Suzuki, Kenichi Watanabe, Prasanna krishnamurthy
Journal: Circulation (2014)

14-3-3 family members are dimeric phosphoserine-binding proteins participating in signal transduction and checkpoint control pathways. We have previously reported that 14-3-3 protein protects against pressure-overload induced heart failure in mice. However, the role of 14-3-3 protein in myocardial infarction (MI)-induced cardiac dysfunction still remains to be determined. MI was induced by ligating the left anterior descending (LAD) coronary artery in wild type (WT) and cardiac-specific expression of dominant-negative 14-3-3 protein mutant (DN14-3-3) mice. One week later sham surgery; there was no significant difference in cardiac function in WT and DN14-3-3 mice. DN14-3-3 mice with MI showed increased mortality and reduced left ventricular ejection fraction and fractional shortening as compared to WT mice. DN14-3-3 mice showed increased markers of maladaptive cardiac remodeling, cardiac hypertrophy, inflammation, fibrosis and cardiac cell apoptosis as compared to their WT counterparts. Mechanistically, DN14-3-3 mice exhibited increased activation of endoplasmic reticulum (ER) stress. Interestingly, Stem cells marker (C-Kit, ISL-1) expressions were significantly reduced in DN14-3-3 mice with MI when compared to WT mice. In conclusion, depletion of 14-3-3 protein leads to increased mortality associated with increased cardiac dysfunction and adverse cardiac remodeling in mice subjected to MI, possibly, via exacerbation of ER stress and activation of death signaling pathways and suppression of cardiac regeneration. Thus, identification of drugs that can modulate cardiac 14-3-3 protein levels might provide a novel protective therapy for heart failure.