tbuckley/cabot-search huggingface dataset:
Abstract 293: High-throughput methylation sequencing of targeted genes in breast cancer specimens using nanofluidic PCR prepared libraries
Authors: Jun Wang, Zibo Li, Yepeng Wu, Xinwu Guo, Shengyun Li, Zhi Xiao, Feiyu Chen, Zhongping Deng, Lizhong Dai, Wenjun Yi, Lili Tang
Journal: Cancer Research (2014)

Abstract Breast cancer is a global public health issue as it is the most frequently diagnosed malignancy in women in the Western world with over a million new cases every year worldwide. Previous epigenetic analyses have identified aberrant DNA methylation signatures associated with breast cancer. Methylation alterations resulting in aberrant gene expression are key contributors to breast tumorigenesis. There are more than 100 candidate genes reported throughout the literatures as promoter hypermethylated in breast cancers (Pubmeth web resource). More recently, with the application of genome wide array-based methylation profile analysis and next generation sequencing, hundreds of new methylation candidate genes have been reported to be associated with breast cancer. It is crucial to develop an approach for targeted methylaton resequencing to validate these candidate genes in hundreds of patient samples and develop a breast cancer detection panel for early diagnostics. We have developed a targeted gene bisulfite-sequencing approach using a nanofluidic platform, the Access Array™ system, which enables simultaneous amplification of 48 genetic regions (amplicon) from 48 samples in parallel. We have selected 48 breast cancer associated methylation candidate genes, each commonly reported by multiple literatures. Bisulfite-specific-sequencing (BSP) primers were designed for the promoter regions of these genes. In addition, we have applied a primer design strategy that incorporates sample-specific barcodes and Illumina (Miseq) sequencing adaptors into each amplicon, removing the need for additional library preparation before sequencing. We will present methylation sequencing data collected from amplicon libraries generated using the Access Array system. The 48 promoter regions have been amplified in paired tumor/normal tissues and plasma DNA from 48 breast cancer patients. Our results indicate the excellent utilization of this approach in the validation of methylation biomarkers and selection of detection panel for breast cancer. Citation Format: Jun Wang, Zibo Li, Yepeng Wu, Xinwu Guo, Shengyun Li, Zhi Xiao, Feiyu Chen, Zhongping Deng, Lizhong Dai, Wenjun Yi, Lili Tang. High-throughput methylation sequencing of targeted genes in breast cancer specimens using nanofluidic PCR prepared libraries. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 293. doi:10.1158/1538-7445.AM2014-293
Postcodes as useful markers of social class: population based study in 26 000 British households Commentary: Socioeconomic position should be measured accurately
Authors: J. Danesh, S. Gault, J. Semmence, P. Appleby, R. Peto, Y. Ben-Shlomo, G. D. Smith
Journal: BMJ (1999)

with live attenuated micro-organisms. 3 7 8 We believe that only one case of paralytic paralysis associated with oral poliomyelitis vaccine has been reported in a child with HIV infection from Romania. 12 Thus oral poliomyelitis vaccine seems to be safe when given during the first year of life. 1 7 10 13 To our knowledge, our case is the first report of poliomyelitis associated with poliomyelitis vaccination in a child infected with HIV from Africa.In countries where HIV infection is endemic and the risk of infection with wild type poliomyelitis virus is high, the benefits of immunisation outweigh the apparently low risk of paralysis due to vaccination with oral poliomyelitis vaccine.
Identity Matters (or Does It?)
Authors: Z DeFries
Journal: JAMA (1998)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Applications to US medical schools fall by a fifth
Authors: F. Charatan
Journal: BMJ (2000)

Trypanosomiasis, also known as African sleeping sickness, has been reported in the United Kingdom for the first time in four years—in two men returning from Africa.

In the first case, a man in his 30s was admitted with the disease to the Hospital for Tropical Diseases in London on 21 October after returning from a holiday in Zambia. Dr Peter Chiodini and his registrar at the hospital phoned several other hospitals and healthcare institutions in the United Kingdom and elsewhere in search of suramin, the drug used for …
Abstract 2026: Analysis of the impact of compounds derived from marine organisms on 3D co-spheroids generated from multiple myeloma cells and stromal cell types
Authors: Jayanthi Ganesan, Holger Weber, Sarah Umber, Oliver Siedentopf, Daniel Feger, Luis F. García-Fernández, Miguel Aracil Ávila, Michael H.G. Kubbutat, Jan E. Ehlert
Journal: Cancer Research (2014)

Abstract Multiple myeloma (MM) is a severe leukemic disease of plasma B-cells in the bone marrow to which there is currently no cure. Stromal components such as mesenchymal stromal cells (MSC) or endothelial cells (EC) are suspected to play a central role in mediating resistance during treatment of patients. In the attempt to identify novel drugs for the treatment of MM in extracts of marine organisms, we established 3D co-spheroid systems which would allow to factor in micro-environmental signals already in the early phase of drug discovery. We report on the establishment of both an angiogenesis and a proliferation assay using 3D co-spheroids combining MM cells together with either endothelial or mesenchymal stromal cells, respectively. These co-spheroid systems were applied to characterize a variety of compounds for their anti-angiogenic activity on EC or anti-proliferative activity on MM as well as MSC. Funded by European Union Seventh Framework Program (FP7/2007-13) under Grant Agreement Nr.278570 Citation Format: Jayanthi Ganesan, Holger Weber, Sarah Umber, Oliver Siedentopf, Daniel Feger, Luis F. García-Fernández, Miguel Aracil Ávila, Michael H.G. Kubbutat, Jan E. Ehlert. Analysis of the impact of compounds derived from marine organisms on 3D co-spheroids generated from multiple myeloma cells and stromal cell types. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2026. doi:10.1158/1538-7445.AM2014-2026
News
Authors: J Warden
Journal: BMJ (1992)

Euthanasia around the worldEuthanasia arouses strong feelings among both doctors and the public.Last year it reached political significance in the United States, where an attempt to legalise doctor assisted suicide was only narrowly defeated.The media coverage of the debate was enor- mous.This year there are likely to be more attempts worldwide to define legally doctors' obligations to their dying patients.We review the position im several countries.Japan
Abstract 17076: A Novel Polymer-Free Ciglitazone-Eluting Stent Inhibits Neointimal Proliferation Stronger than Sirolimus-Eluting Stents: An Experimental Study Using Optical Coherence Tomography in Rabbit Iliacal Arteries
Authors: Tudor C Poerner, Sylvia Otto, Sabine Bischoff, Diana Muhlstadt, Bjorn Goebel, Christian Jung, Hans R Figulla
Journal: Circulation (2010)

Background: Drug-eluting stents (DES) are currently the best choice to reduce restenosis at long-term follow-up after percutaneous coronary interventions. Optical coherence tomography (OCT) is a high-resolution intravascular imaging technique that accurately distinguishes the various layers of the arterial wall, plaque composition and stent struts, allowing to precisely quantify the extent of neointimal proliferation . Objective: To investigate ciglitazone, a peroxisome proliferator-activated receptor-gamma agonist as a novel stent coating. Methods: Bare metal stents Yukon Choice 2.5/12 mm with microporous surface (BMS) were polymer-free coated with either sirolimus (SES) or ciglitazone (CES) at 225 mcg/cm. Twelve New Zealand White rabbits fed with high cholesterol western diet underwent implantation of different stents in both common iliacal arteries via the carotid artery. The animals were equally divided into 2 groups: BMS vs. SES and BMS vs. CES. After 90 days of dual antiplatelet therapy stents were ...
Abstract 3531: MicroRNA delivery by bionanoparticles: regulation of the liver cancer susceptibility gene MICA expression in hepatocytes
Authors: Motoko Ohno, Motoyuki Otsuka, Takahiro Kishikawa, Takeshi Yoshikawa, Akemi Takata, Kazuhiko Koike
Journal: Cancer Research (2014)

Abstract Hepatocellular carcinoma (HCC) is an important issue to public health of the world. We recently reported by genome wide association study (GWAS) analyses that a single nucleotide polymorphism (SNP) in the promoter region of the MHC class I polypeptide-related sequence A (MICA) gene that is significantly associated with the risk of hepatitis-virus-related HCC. Because there was a correlation between the expression levels of MICA in the serum depending on the different alleles at the SNP site and the risk of HCC, regulation of MICA expression levels may be useful in the prevention of HCC. Here we show that MICA protein expression in HCC cells can be regulated by the microRNA (miR) 25-93-106b cluster. By use of luciferase assay and FACS analyses, it was confirmed that forced expression of the miR 25-93-106b cluster significantly suppressed MICA expression, whereas inhibition of this miR cluster increased MICA expression in cells that stably express MICA. Furthermore, to deliver efficiently miRNAs into hepatocytes specifically, we developed bio-nanocapsules containing miR93 inside, utilizing hepatitis B virus envelope L-protein. MICA expression levels were indeed suppressed after the delivery of miR93 by these bionanoparticles into hepatocytes. Besides, the binding ability of NKG2D (a receptor of MICA) and in vivo cell-killing by NK cells were changed proportionally to miRNA-induced MICA expression levels. These results suggest that the modulation of MICA expression levels in hepatocytes by delivering miRNAs using these bionanocapsules may be a novel and effective approach to prevent hepatitis-virus-related HCC. Citation Format: Motoko Ohno, Motoyuki Otsuka, Takahiro Kishikawa, Takeshi Yoshikawa, Akemi Takata, Kazuhiko Koike. MicroRNA delivery by bionanoparticles: regulation of the liver cancer susceptibility gene MICA expression in hepatocytes. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3531. doi:10.1158/1538-7445.AM2014-3531
Abstract 6162: It is not (just) the Increased Afterload that Causes the Right Heart to Fail in Pulmonary Hypertension
Authors: Harm J Bogaard, Ramesh Natarajan, Donatas Kraskauskas, Kohtaro Abe, Joe M Mc Cord, Norbert F Voelkel
Journal: Circulation (2008)

Background: An increased afterload is generally viewed as the single cause of right ventricular (RV) failure in pulmonary hypertension (PH). We hypothesized that per se pressure-independent feature...
Book: Diabetes: Emergency and Hospital Management
Authors: R. Matz
Journal: BMJ (1999)

Simon R Page, George M Hall BMJ Books, £25, pp 248 ISBN 0 7279 1229 1 Rating: ![Graphic][1]</img>![Graphic][2]</img>![Graphic][3]</img> What a pleasure to read a two authored work that doesn't equivocate and expresses a consistent viewpoint (as opposed to standard multiauthored texts or dry consensus statements). Better yet, it is light and well written and admirably addresses important aspects of emergencies in diabetes care and hospital management. Several unique and attractive features of this publication warrant comment It's obvious that the authors have extensive hands-on experience with many of the problems dealt with. Accordingly, their clinical vignettes are associated with practical management gems at every turn. Their coverage of surgery … [1]: /embed/inline-graphic-1.gif [2]: /embed/inline-graphic-2.gif [3]: /embed/inline-graphic-3.gif
Abstract 4230: 5α-dihydrotestosterone down-regulates HER2 and sensitizes breast cancer cells to Herceptin-induced apoptosis <i>in vitro</i>
Authors: Aisha Sultana, Zulfiqar Ali Naqvi, Shaista Khan, Anwar Ali Siddiqui, El-Nasir Lalani
Journal: Cancer Research (2014)

Abstract Androgen inhibits growth of malignant breast cancer (BCa) cells by mechanisms that remain poorly defined. Here, we have investigated how androgen suppress proliferation and induces apoptosis in BCa cells. Treatment of MCF-7 cells with 1nM 5α-dihydrotestosterone (DHT) inhibited cell proliferation as well as endogenous HER2 expression, both at mRNA and protein levels, suggesting a link between androgen receptor (AR) and HER2 signaling pathways. Expression of HER2 and AR was also determined in patients with BCa. We found a survival advantage of AR expression in patients with HER2 positive BCa, whereas, loss of AR expression was associated with poor survival. This finding would suggest that AR loss may provide a growth advantage to HER2 over-expressing tumors. We showed the functional consequence of the stimulation of AR-signaling and inhibition of HER2-signaling on breast epithelial cell growth and on expression of 84 genes involved in BCa and estrogen-mediated signaling pathway. Exposure to a combination therapy of DHT and Herceptin resulted in additive anti-proliferative effect in human MCF-7 cells as compared to either agent alone. Furthermore, combined treatment of DHT with Herceptin reduces the expression of genes predominantly involved in proliferation, invasion, anti-apoptosis and cell cycle regulation. These findings provide the key insights into how Herceptin and DHT produce anti-proliferative effect in BCa cells. In conclusion, adding DHT to Herceptin may provide a rational treatment option for BCa, not only by direct inhibition of cell proliferation, but also causing changes in the expression of many genes that are critically involved in the control of proliferation. Establishment of any interaction between AR and HER2 may improve the understanding of carcinogenesis and will provide helpful information in the development of novel preventive and therapeutic strategies. Citation Format: Aisha Sultana, Zulfiqar Ali Naqvi, Shaista Khan, Anwar Ali Siddiqui, El-Nasir Lalani. 5α-dihydrotestosterone down-regulates HER2 and sensitizes breast cancer cells to Herceptin-induced apoptosis in vitro. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4230. doi:10.1158/1538-7445.AM2014-4230
Identification of Collaborating Oncogeneic Events Leading to Disease Progression in Myeloma Cases with a t(4;14) and t(11;14) Using SNP and Gene Expression Arrays.
Authors: Matthew W. Jenner, Brian A. Walker, Paola E. Leone, David Gonzalez, Fiona M. Ross, Cheng Li, Faith E. Davies, Gareth J. Morgan
Journal: Blood (2005)
Mutations in NYX, encoding the leucine-rich proteoglycan nyctalopin, cause X-linked complete congenital stationary night blindness
Authors: N.Torben Bech-Hansen, Margaret J. Naylor, Tracy A. Maybaum, Rebecca L. Sparkes, Ben Koop, David G. Birch, Arthur A.B. Bergen, Clemens F.M. Prinsen, Robert C. Polomeno, Andreas Gal, Arlene V. Drack, Maria A. Musarella, Samuel G. Jacobson, Rockefeller S.L. Young, Richard G. Weleber
Journal: Nature Genetics (2000)
Adiponectin and Risk of Coronary Heart Disease—Reply
Authors: Tobias Pischon, Gokhan S. Hotamisligil, Eric B. Rimm
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
649. Repression of Vascular Endothelial Growth Factor-A Over-Expression in Glioblastoma Cells Using Engineered Zinc-Finger Transcription Factors
Authors: Alberto Auricchio, Gabriella Cotugno, Pietro Formisano, Francesco Beguinot, Enrico M. Surace, Roy Pollock
Journal: Molecular Therapy (2004)

Insulin gene replacement therapies for type I diabetes, that result in ectopic insulin production from tissues engineered with viral vectors, lack the precise temporal control associated with natural hormone secretion. A potential solution to this problem is to direct gene transfer to insulin target cells and introduce constructs that activate the insulin signaling pathway in response to an exogenously administered drug. Insulin regulates metabolism and glucose homeostasis through binding to a specific membrane receptor (IR) that displays tyrosine kinase activity. We constructed a chimeric insulin receptor (Fv2IRE) whose dimerization and subsequent activation can be induced by a small molecule dimerizer drug, AP20187. This system should activate the IR signaling pathway in insulin target cells with kinetics similar to those of the natural hormone. The Fv2IRE chimeric receptor, which binds AP20187, was introduced in human HepG2 hepatocytes and primary human fibroblasts using AAV2/1 vectors. AP20187 is able to induce Fv2IRE dimerization and transphosphorylation in a dose dependent way. This activation leads to AP20187 dose dependent tyrosine phosphorylation of the canonic IR substrate IRS1. This occurs independently of the activation of the endogenous IR expressed in HepG2 cells. AP20187 activation of Fv2IRE induces glycogen synthase activation and proliferation in AAV transduced- cells.
Use of Antibiotics and Risk of Cancer
Authors: Spencer E Harpe
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Studies on the Thymus of the Mammal. XII. Histochemistry of the Thymuses of C57BL/6 and AKR Strain Mice&lt;xref ref-type="fn" rid="FN1"&gt;2&lt;/xref&gt;
Authors: Christianna Smith
Journal: JNCI Journal of the National Cancer Institute (1961)

The cortex of the thymus of young AKR strain mice was characterized by a generalized distribution of alkaline phosphatase activity. In unfixed, frozen sections cut in the cryostat, in addition to the reactive arterial endothelium, epithelial and mesenchymal reticular cells and lymphocytes were positive. In contrast, the C57BL/6 cortical cells were mostly negative except for scattered reticular cells and the endothelium. The medulla of thymuses from both strains contained strongly reactive oval cells. These cells were not as prominent in the medulla of strain AKR because it was populated with more reactive cells than in the medulla of strain C57BL/6. Focal areas of deeply colored cells also distinguished the AKR medulla. The cortex and the corticomedullary zone of the AKR thymus were usually characterized by many cells that were browned with the acid phosphatase technique; the medulla was almost devoid of positive cells. With the same technique, the C57BL/6 thymus was distinguished by a medulla and a corticomedullary region with scattered positive cells; the cortex was mostly negative. The thymuses of strain C57BL/6 retired breeders, though more or less involuted, were recognized by a pattern of alkaline phosphatase activity similar to that of the young mice. Those of strain AKR mice varied according to size, whether involuted or hyperplastic, and activity. Approximately half of the lobes of these thymuses from old AKR mice were more strongly reactive than the thymic lobes from young mice. In the 5-week-old mice, thymuses from the male and female AKR mice were heavier than those of the C57BL/6. Thymuses of the males of both strains weighed less than those of the females.