tbuckley/cabot-search huggingface dataset:
Abstract 5425: L-amino acid oxidase from Crotalas adamanteus venom induces caspase-independent apoptosis in human NUGC-3 gastric cancer cells
Authors: Jian Ding, Boon-Huat Bay, P Gopalakrishnakone
Journal: Cancer Research (2014)

Abstract L-amino acid oxidases (LAAOs), which are largely present in snake venoms, are a group of flavoenzymes, known to catalyze oxidative deamination of L-amino acid substrates, resulting in the formation of corresponding α-keto acids, hydrogen peroxide and ammonia. LAAOs have attracted interest in biomedical and pharmacological research because of their antimicrobial, anti-HIV, anticoagulant, inhibition of platelet aggregation and apoptotic-inducing activities. The aim of this study is to evaluate the anticancer effects of LAAO from Crotalas adamanteus snake venom in human NUGC-3 gastric cancer cells in vitro. Cell viability and cytotoxicity were determined by Alamar Blue and Lactate Dehydrogenase assays. Cell cycle phases were analyzed by flow cytometry. Apoptosis was identified by flow cytometric analysis with Annexin-V and PI staining. The morphological changes were observed by fluorescence microscopy, transmission electron microscopy and scanning electron microscopy. The ROS level was quantified by DCFDA staining as well as malondialdehyde formation. The results showed that LAAO reduced cell viability and induced cytotoxicity in a dose-dependent manner. There was associated cell cycle arrest at the G2/M phase. Morphological changes induced by LAAO treatment included membrane blebbing, cell shrinkage and chromatin condensation. Apoptosis induced by LAAO in NUGC-3 gastric cancer cells as evidenced by Annexin-V and PI positive staining, was found to be mediated via a caspase-independent pathway without activating caspase-3 and Poly ADP-ribose polymerase. Moreover, a concomitant increase of intracellular oxidative stress and the translocation of the apoptosis inducing factor (AIF) from mitochondria to the nucleus were observed. It would appear that LAAO derived from the Crotalas adamanteus venom, induced a caspase-independent apoptotic pathway which was incited by ROS and AIF translocation. This study provides new biological insights into the application of LAAO from a snake venom in cancer therapeutics. Citation Format: Jian Ding, Boon-Huat Bay, P Gopalakrishnakone. L-amino acid oxidase from Crotalas adamanteus venom induces caspase-independent apoptosis in human NUGC-3 gastric cancer cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5425. doi:10.1158/1538-7445.AM2014-5425
Abstract 10589: Univentricular Hearts in Denmark 1977-2009: Decreasing Incidence, Improving Survival, and Several Predictors of Survival
Authors: Lars Idorn, Morten Olsen, Soren P Johnsen, Annette Jensen, Klaus Juul, Jesper Reimers, Lars Sondergaard
Journal: Circulation (2011)

Introduction: The purpose of this national population-based study was to describe changes in incidence and survival in functional univentricular heart (UVH) patients in Denmark from 1977 to 2009. H...
PD4-3-5: Phase I/II study of oral TS-1 and gemcitabine in elderly patients with advanced non-small-cell-lung cancer (NSCLS): Thoracic Oncology Research Group Study 0502
Authors: Nobuhiko Seki, Takashi Seto, Hiroaki Okamoto, Takashi Ogura, Masahiko Shibuya, Yuichi Takiguchi, Tetsu Shinkai, Noriyuki Masuda, Koshiro Watanabe
Journal: Journal of Thoracic Oncology (2007)
903-13 Selenium-Containing Antioxidant (Ebselen) Improves the Response to Balloon Injury in the Pig Coronary Model: Effects on Vascular Remodeling
Authors: Ron Waksman, Keith A. Robinson, Joel Schneider, Gustavo Cipolla, Hiroyuki Masayasu, Spencer B. King
Journal: Journal of the American College of Cardiology (1995)
Effective Commissioning: Lessons from Purchasing in American Managed Care
Authors: J. Dixon
Journal: BMJ (1998)

Donald W Light

Office of Health Economics, £7.50, pp 90

ISBN 1 899040 552

In some ways the NHS has changed enormously since 1991. Purchasers were put in the driving seat, needs were assessed, explicit priorities were drawn up, shifts in investment were made, and fundholders wrought changes in areas that had irked them for years. But in other ways, surprisingly little was achieved considering the changes in funding and organisation that went into making the split work.

Why? The instrument of change was purchasing, and this dog not only failed to bite but didn't even bark loudly enough to wake up …
551 Five-year treatment with high-dose UDCA in PSC
Authors: R.G. Olsson, K.M. Boberg, O. Schaffalitzky de Muckadel, S. Lindgren, R. Hultcrantz, G. Folvik, H. Bell, G. Kristiansen, J. Matre, A. Rydning, O. Wikman, A. Danielsson, H. Sandberg-Gertzen, K.A. Ung, A. Eriksson, L. Loof, H. Prytz, U. Broome
Journal: Journal of Hepatology (2004)
Symptoms of irritable bowel syndrome in ulcerative colitis in remission
Authors: B Isgar, M Harman, M D Kaye, P J Whorwell
Journal: Gut (1983)

The prevalence of symptoms suggestive of a motility disturbance similar to the irritable bowel syndrome was investigated in 98 patients with ulcerative colitis in remission, and a similar number of age- and sex-matched healthy controls. Thirty-three per cent of patients compared with 7% of controls fulfilled the criteria for such a syndrome (p&lt;0·01). Contrary to expectations these symptoms were not confined to patients with distal disease. Other symptoms such as constipation were also very common in the colitic group (31%).
Melanomas That Defy Clinical Recognition
Authors: Judd W. Moul
Journal: JAMA (1992)

<h3>To the Editor.</h3> —We read with interest the article by Drs Anderson and Silvers<sup>1</sup>on melanoma that defied clinical recognition before final pathologic examination of the excised lesions. They described 13 cases, most of which were amelanotic, located on the extremities, neck, scalp, back, and ears, and were initially diagnosed as benign lesions or basal cell carcinomas. These were genuine false-negative melanomas in which melanoma was not even remotely considered as a diagnostic possibility before biopsy. The benign-appearing lesions reported by Anderson and Silvers had a hyperkeratotic or verrucous appearance and were diagnosed as seborrheic keratosis or verruca vulgaris. To add to the experience of Anderson and Silvers, we have recently treated an 85-year-old white man who presented with a verrucous-appearing scrotal lesion that on excisional pathology revealed primary scrotal melanoma, although melanoma was not considered before biopsy. The man had a 1-year history of a gradually enlarging hyperkeratotic,
A matter of life and death: cardiac myocyte apoptosis and regeneration
Authors: Bernardo Nadal-Ginard, Jan Kajstura, Piero Anversa, Annarosa Leri
Journal: Journal of Clinical Investigation (2003)

It is also important to note that the proapoptotic Vpr protein is not the only viral mitochondrion-targeted protein to be identified as a virulence factor (9).It will be interesting to learn which other viruses produce similar mitochondrion-targeted, apoptosisregulatory, disease-relevant proteins, all of which could constitute promising pharmacological targets.
IT-11 * A PHASE I/ II CLINICAL TRIAL OF CT-011 (PIDILIZUMAB) IN DIFFUSE INTRINSIC PONTINE GLIOMA AND RELAPSED HIGH GRADE GLIOMA: A PRELIMINARY REPORT
Authors: I. Fried, M. Weintraub, T. Ben Ami, R. Shen, M. Benifla, A. Mordechai, A. Lossos
Journal: Neuro-Oncology (2014)

BACKGROUND: Diffuse intrinsic pontine glioma (DIPG) and relapsed high grade glioma (HGG) are incurable diseases with an event free survival of 6-8 months. Anti-Programmed Death 1 (anti-PD1) antibodies have recently emerged as a promising treatment modality in multiple cancer types. Clinical studies evaluating anti PD1 antibodies in the setting of primary brain tumors have not yet been reported. Pidilizumab (CureTech, Yavne, Israel) is an anti- PD1 humanized immunoglubulin G1 monoclonal antibody. This investigator-initiated clinical trial evaluates pidilizumab in malignant gliomas (NCT01952769). METHODS: Patients older than 3 years treated with not more than 2mg/M2 of dexamethasone per day are included. Patients with newly diagnosed DIPG are recruited for biweekly pidilizumab within the first month after completion of radiotherapy. Patients with HGG relapsed after standard treatment are recruited for biweekly pidilizumab as an add-on therapy to bevacizumab within 3 months of recurrence. The study was opened in February 2014. RESULTS: To date, 6 patients have been enrolled: three with newly diagnosed DIPG, two with HGG and one with relapsed DIPG (bevacizumab + pidilizumab). A total of 30 cycles of Pidilizumab (range 2- 14) have been administered. Treatment was well tolerated with transient fatigue as the main side effect. Administration of pidilizumab less than 10 days after bevacizumab resulted in a grade 3 blood pressure elevation in one patient. This did not recur when the interval between agents was increased. One patient withdrew because of parents' decision. One patient with HGG progressed and died within 2.5 months from enrollment. Two patients had a significant tumor shrinkage along with clinical improvement. Scheduled imaging evaluation is pending in the rest. CONCLUSION: The trial is ongoing. This report is confined to schedule and initial enrollment results.
Biblical Fiery Serpents
Authors: Marianne W. Chermak
Journal: JAMA (1961)

<h3>To the Editor:—</h3> The article on "Moses, Physician for Typhoid" (JAMA<b>177</b>:68 [Sept. 16] 1961) prompts the hope that a question I have harbored for years may be answered by one of the experts in this field. As with all biblical events, there have been numerous attempts to explain and interpret the "fiery serpents," as well as the "serpent of brass," which Moses put upon the pole (Num. 21:6-9). I have always thought that the "burning or fiery serpents" were lightning, that the pole with the brass top served as a lightning rod, and that whoever was close enough "to look upon it" was protected. After all, "Moses was learned in all the wisdom of the Egyptians" (Acts 7:22). Also, the same Hebrew word for "the burning serpents" is translated "seraphim" in Isa. 6:2 and 6. Since these seraphim came from heaven, this would lend weight to my assumption.
Cell cycle inhibition preserves endothelial function in genetically engineered rabbit vein grafts.
Authors: M J Mann, G H Gibbons, P S Tsao, H E von der Leyen, J P Cooke, R Buitrago, R Kernoff, V J Dzau
Journal: Journal of Clinical Investigation (1997)

We have recently shown that ex vivo gene therapy of rabbit autologous vein grafts with antisense oligodeoxynucleotides (AS ODN) blocking cell cycle regulatory gene expression inhibits not only neointimal hyperplasia, but also diet-induced, accelerated graft atherosclerosis. We observed that these grafts remained free of macrophage invasion and foam cell deposition. Since endothelial dysfunction plays an important role in vascular disease, the current study examined the effect of this genetic engineering strategy on graft endothelial function and its potential relationship to the engineered vessels' resistance to atherosclerosis. Rabbit vein grafts transfected with AS ODN against proliferating cell nuclear antigen (PCNA) and cell division cycle 2 (cdc2) kinase elaborated significantly more nitric oxide and exhibited greater vasorelaxation to both calcium ionophore and acetylcholine than did untreated or control ODN-treated grafts. This preservation of endothelial function was associated with a reduction in superoxide radical generation, vascular cell adhesion molecule-1 (VCAM-1) expression, and monocyte binding activity in grafts in both normal and hypercholesterolemic rabbits. Our data demonstrate that AS ODN arrest of vascular cell cycle progression results in the preservation of normal endothelial phenotype and function, thereby influencing the biology of the vessel wall towards a reduction of its susceptibility to occlusive disease.
Metabolic Responses to Exercise: Effects of endurance training and implications for diabetes
Authors: Erik A Richter, Lorraine Turcotte, Peter Hespel, Bente Kiens
Journal: Diabetes Care (1992)

In this study, some important metabolic responses to exercise will be discussed, and aspects of particular interest for patients with diabetes mellitus will be emphasized. Alterations in the metabolic responses to exercise induced by physical endurance training and consequences of training for metabolism of plasma lipids and lipoproteins will be discussed. Glucoregulation during exercise is not perfect in normal subjects and is less so in patients with diabetes mellitus. For instance, during intense exercise, large increases in the plasma glucose concentration occur and a state of insulin resistance exists for a few hours after intense exercise. Even so, increased sensitivity to insulin is found the day after intense exercise and also shortly after more moderate intensity exercise, both in healthy subjects and in patients with diabetes mellitus. Increased sensitivity to insulin is also found after endurance training, whereas insulin sensitivity is decreased after inactivity. Exercise training increases the ability of muscle to take up and oxidize free fatty acids during exercise and also increases the activity of the enzyme lipoprotein lipase in muscle. The activity of lipoprotein lipase in muscle correlates with muscle insulin sensitivity. This might explain why insulin resistance is often associated with hypercholesterolemia, hypertriglyceridemia, and low high-density lipoprotein cholesterol.
TCT-273 Patient Characteristics that Deter use of a Bleeding Avoidance Strategy during PCI
Authors: Sameer J. Khandhar, Helen Vlachos, Catalin Toma, Faith Selzer, Maria M. Brooks, Sun K. Scolieri, Krishnamurty V. Tummalapalli, Joon S. Lee, Oscar C. Marroquin, John T. Schindler, A J Conrad Smith, Suresh Mulukutla
Journal: Journal of the American College of Cardiology (2013)
Alkaline gastro-oesophageal reflux: dual probe pH monitoring.
Authors: S Y Iftikhar, S Ledingham, D F Evans, S W Yusuf, R J Steele, M Atkinson, J D Hardcastle
Journal: Gut (1995)

Although the aetiology of Barrett's oesophagus or columnar line oesophagus (CLO), remains unknown, bile reflux has been implicated as a factor in its pathogenesis. This study aimed to detect alkaline reflux in gastro-oesophageal reflux patients using dual probe pH monitoring. Thirty patients with histologically diagnosed CLO, 15 age and sex matched patients with oesophagitis (grade 1-3), and 15 healthy volunteers were studied by dual probe, 18 hour pH monitoring and analysis of the bile acid content of oesophageal refluxate. Total acid exposure and acid exposure in the upright and supine postures were greater in CLO subjects than in oesophagitis patients and controls. Furthermore, the number of reflux episodes lasting more than five minutes and the duration of the longest reflux episode were significantly greater in the CLO subjects than the oesophagitis and control subjects. Nine subjects with CLO and oesophagitis, however, were not identified as refluxers, although six had a bile acid concentration in their oesophageal aspirate higher than the 95th centile value of the controls. There was no correlation between the oesophageal pH and the bile acid contents of refluxate. It is concluded that dual probe pH monitoring is not useful in detecting alkaline refluxers. pH monitoring, although the only subjective test available to identify acid refluxers, is not a sufficiently sensitive test with which to define alkaline reflux.
What is the criterion for differentiating chronic hepatitis from compensated cirrhosis? A prospective study comparing ultrasonography and percutaneous liver biopsy
Authors: Stefano Gaiani, Laura Gramantieri, Nicola Venturoli, Fabio Piscaglia, Sebastiano Siringo, Antonia D'Errico, Gianni Zironi, Walter Grigioni, Luigi Bolondi
Journal: Journal of Hepatology (1997)
Abstract 4523: Stool proteomics reveals new candidate biomarkers for colorectal cancer screening
Authors: Linda JW Bosch, Meike de Wit, Gideon Oudgenoeg, Annemieke Hiemstra, Sandra Mongera, Sander R. Piersma, Thang V. Pham, Nicole CT van Grieken, Jochim S. Terhaar sive Droste, Frank A. Oort, Sietze T. van Turenhout, Ilhame Ben Larbi, Chris JJ Mulder, Beatriz Carvalho, Connie R. Jimenez, Remond JA Fijneman, Gerrit A. Meijer
Journal: Cancer Research (2012)

Abstract Background Colorectal Cancer (CRC) screening can save many lives. Many invitational large scale screening programs worldwide use stool tests like the fecal immunochemical tests (FIT), which detects human hemoglobin. Tumor-specific biomarkers have the potential to improve the performance of these tests but so far, no protein-based fecal test has proved better than the FIT. Although most biomarker discoveries are done in tumor-tissues, the presence and/or chemical nature of biomarkers may be different in samples that ultimately will be used for screening like stool. Measuring biomarkers directly in stool samples may therefore yield candidate CRC biomarkers that are stable in the fecal environment. Aim The aim of the present study was to identify tumor-specific protein based biomarkers for the early detection of CRC, by applying in-depth proteomics to stool samples from CRC patients and healthy controls. Material and method Stool samples were obtained from 10 subjects with negative colonoscopy and from 12 CRC patients. Proteins were analyzed by in-depth proteomics using gel electrophoresis and nano Liquid Chromatography coupled to tandem mass spectrometry (nano LC-MS/MS). Resulting MS/MS spectra were searched against the human IPI database (version 3.62). Proteins were analyzed by hierarchical cluster analysis and visualized in a heat map. Non-paired statistical analysis of spectral count data from human proteins was performed using a beta-binomial test. Verification of candidate biomarkers was performed by Selected Reaction Monitoring Mass Spectometry (SRM-MS). Results In total 830 human proteins were identified of which 221 were present at different levels in stool samples from CRC patients compared to control subjects. Of these, 134 proteins were significantly enriched in CRC. Unsupervised hierarchical cluster analysis revealed two clusters. One cluster contained nine CRC stool samples, the other cluster contained all ten control stool samples together with three CRC stool samples. SRM-MS analysis of selected candidate biomarkers on the same stool samples verified the results obtained by LC-MS/MS. Conclusion Proteome profiling on stool revealed 134 proteins significantly enriched in CRC compared to control stool samples, of which a sub set could be verified by SRM-MS. Validation in an independent series of stool samples collection (n=200) by SRM-MS is in process. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4523. doi:1538-7445.AM2012-4523
Infantile Methemoglobinemia Caused by Carrot Juice
Authors: James P. Keating, Mary Elizabeth Lell, Arnold W. Strauss, Harold Zarkowsky, George E. Smith
Journal: New England Journal of Medicine (1973)

THE toxic threat of nitrate contained in commonly ingested vegetables has recently been reviewed.1 Concern has been stimulated by reports of methemoglobinemia in German and French infants after ingestion of spinach puree2 and carrot soup.3 In the following case cyanosis developed after ingestion of fresh carrot juice by an American infant.Case ReportA 2-week-old black male infant (S.L.C.H. 72–6245) was taken to the Emergency Room because his grandmother had noted that his lips and nail beds had a "dark color." In the previous 24 hours he had consumed 500 ml of carrot juice. Fever, diarrhea, lethargy and the administration . . .
Intravenous Epinephrine Therapy in Anaphylaxis
Authors: Jerrold H. Levy
Journal: JAMA (1983)

<h3>To the Editor.—</h3> In a recent case report by Sullivan (1982;248:2161), he cautions practitioners against the use of intravenous epinephrine chloride in anaphylaxis. The patients who received epinephrine all had baseline systolic BPs of 128 and 100 mm Hg and then received full resuscitation doses of 0.5 mg. There was no mention of the BP after epinephrine administration and during the arrhythmias that ensued. In the studies cited,<sup>1</sup>two of the three patients who had severe cardiovascular collapse required repeated 0.5-mg boluses of epinephrine and volume expansion to restore BP to baseline values. Although arrhythmias may occur with anaphylaxis, it is not clear whether this is because of the coronary vasoconstrictor effects of mediators such as histamine or leukotrienes, hypotension, or epinephrine.<sup>2,3</sup>If epinephrine is administered to any patient, monitoring is essential irrespective of the condition. We routinely administer 1 to 2 μg/min of epinephrine to separate patients from
Attitudes of Patients with Amyotrophic Lateral Sclerosis and Their Care Givers toward Assisted Suicide
Authors: Linda Ganzini, Wendy S. Johnston, Bentson H. McFarland, Susan W. Tolle, Melinda A. Lee
Journal: New England Journal of Medicine (1998)

Amyotrophic lateral sclerosis (ALS) is a neuromuscular disease that causes gradual paralysis, respiratory failure, and death, usually within three to five years after it has been diagnosed. Between 1995 and 1997, we surveyed patients with this disease in Oregon and Washington, as well as their family care givers, in order to determine their attitudes toward assisted suicide. Patients were considered to be willing to contemplate assisted suicide if they agreed with the statement, "Under some circumstances I would consider taking a prescription for a medicine whose sole purpose was to end my life," and disagreed with the statement, "I would never request or take a prescription for a medication whose sole purpose was to end my life." The Oregon Death with Dignity Act, which legalized physician-assisted suicide, was approved by Oregon voters in 1994 but did not go into effect until October 1997, after data collection for this study had been completed.
REDUCED LYSYL OXIDASE EXPRESSION IS ASSOCIATED WITH REDUCED TYPE I COLLAGEN AND INCREASED TYPE III COLLAGEN DEPOSITION IN MYXOMATOUS MITRAL VALVE DEGENERATION
Authors: Meerarani Purushothaman, Purushothaman K-Raman, David Adams, Federico Milla, Patrick A. Lento, Richard R. Peralta, Samin Sharma, Pedro Moreno
Journal: Journal of the American College of Cardiology (2012)
746 INDUCTION MAINTENANCE THERAPY IN CHRONIC HEPATITIS B; STEP-DOWN FROM COMBINATION LAMIVUDINE AND TENOFOVIR TO LAMIVUDINE MONOTHERAPY – A POTENTIAL NEW TREATMENT STRATEGY
Authors: U.S. Gill, L. Payaniandy, D. Payaniandy, J. Schulz, V.A. Ross, Y.N. Kallis, P. Kooner, R.T.C. Marley, P.T.F. Kennedy, G.R. Foster
Journal: Journal of Hepatology (2013)
Continuous Emotional Support During Labor
Authors: Desmond P. J. Barton
Journal: JAMA (1991)

To the Editor.  — I write concerning the report by Kennell et al1regarding the value of emotional support for women in labor that can be provided by a sympathetic and informed femaledoula. This draws attention to a philosophy of the management of labor to which I had recently been exposed during a 2-year period in the National Maternity Hospital in Dublin, Ireland.  Although the probable importance of emotional support in labor provided by a personal attendant seems to have been confirmed in some populations in recent studies,2it is not a new concept and, in fact, has been a central feature of the management of labor in the Dublin institution.3Reports from the National Maternity Hospital have emphasized the low intervention rate and low cesarean section rate.4This management, applicable to nulliparous patients in spontaneous labor with a single gestation and cephalic presentation,3