tbuckley/cabot-search huggingface dataset:
520. Canine Neural Progenitor Cells: Phenotypic Markers In Vitro and In Situ
Authors: Damien D. Pearse, Bas Blits, Mary B. Bunge, Patrick M. Wood, Andre R. Sanchez, Andres Hurtado, Enrique P. Lopez, Oudega Martin
Journal: Molecular Therapy (2004)

The transplantation of Schwann cells (SCs) and olfactory ensheathing glia (OEG) have shown promise as therapeutic interventions for spinal cord injury (SCI) repair. The current study sought to identify novel sites of transplantation for these glial cells, as opposed to direct intraspinal cord injection, that would be less surgically invasive while permitting their migration to the location of SCI where they could facilitate repair. Adult female Fischer rats first received a moderate contusion of the thoracic (T8) spinal cord. One week later, cultured SCs and OEG, previously ex vivo transduced with lentiviral vectors encoding green fluorescent protein (GFP) to allow their identification, were transplanted by injection (6×105 in media) into the following regions: the (i) injury epicenter, (ii) sub-dural space above the injury site, (iii) dorsal artery immediately rostral to the injury site, (iv) cisterna magna and, (v) lumbar sac. An additional strategy sought to utilize migration of endogenous SCs from the periphery into the injury site, by transducing them through direct injection of lentiviral-GFP into (vi) the dorsal roots innervating the injured spinal cord segment. A 2.5 cm segment of the spinal cord encompassing the injury epicenter was evaluated 10 days post-injection for migration of GFP+ glia to the injury site. Very few GFP-OEG were observed in the injured spinal cord in all transplantation paradigms, indicating either poor survival or migration. Similar results were also obtained with SCs, after transplantation into the cisterna magna, lumbar sac or into the sub-dural space above the injury site. However, transplantation of SC into the dorsal artery did allow modest migration to the injury site, though cell numbers were significantly less in comparison to direct intraspinal cord injection. A substantial number of GFP-SCs were observed within the dorsal columns and dorsal horn following transduction of the dorsal roots; from which they had probably they had migrated. As a control, direct injection of lentiviral-GFP into the injured spinal cord resulted in very little cellular transduction. In conclusion, harnessing the migratory response of endogenous SCs appeared to be the most efficacious of the alternative cellular delivery approaches examined and opens a new possibility for combinatory cellular and gene therapy for SCI repair. (ISRT; CRPF; The Miami Project to Cure Paralysis; NIH/NINDS # 09923)
48. In Vivo and In Situ Delivery of Huntingtin-Specific Single-Chain Fv Intrabody Genes as Therapeutic Agents for Huntington's Disease
Authors: Todd W. Miller, William J. Wolfgang, Kevin Manley, Yangsheng Zhou, Chun Zhou, Jack M. Webster, Nicholas D. Mazarakis, Anne Messer
Journal: Molecular Therapy (2004)

Huntington's disease (HD) is one of several neurodegenerative disorders caused by expansion of a CAG repeat. The resulting expanded polyglutamine tract causes the huntingtin protein to form insoluble aggregates and participate in aberrant protein-protein interactions. Therapeutic intervention to prevent aggregation, reduce abnormal interactions, and allow proper proteolytic processing may reduce HD pathogenesis. Intrabodies (single-chain Fv antibody fragments) can bind with high specificity and affinity to intracellular targets, potentially altering their folding and interactions, and serving as curative agents and/or tools for drug discovery in an array of neurological disorders associated with altered protein structures. We have previously demonstrated that an intrabody targeting the N-terminus of huntingtin (upstream of the polyglutamine tract) dramatically reduces aggregate formation in HEK-293, BHK-21, and COS-7 cell lines, and provides functional protection against expanded polyglutamine toxicity in organotypic brain slice cultures. Transient dual expression of both intrabody and huntingtin exon 1 (httex1)-GFP proteins from a single vector in a rat striatal cell model (ST14A) shows that the intrabody enhances protein turnover, measured as a decrease in the amount of soluble httex1-GFP; effects were even more dramatic in the HEK293T cell line. We are now using recombinant equine infectious anemia virus (EIAV; pONY8.7), pseudotyped with either VSV-G or Rabies-G, encoding intrabodies alone or in bicistronic vectors to stably transduce neuronal cell lines and brain cells. Viral titers are 108-9 IU/ml for all constructs.
Study Evaluates Shortened TB Drug Regimen
Authors: M.J. Friedrich
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Specialized Dementia Care Units
Authors: Dorothy H. Coons
Journal: Annals of Internal Medicine (1991)

Contributors: Kathy Boling, Jaikishan R. Desai, Deborah T. Gold, Lisa Gwyther, Lorraine G. Hiatt, Nancy L. Mace, Laura J. Mathew, Nancy K. Orr-Rainey, Lynn Ritter, Philip D. Sloane, William D. Weissert
Central hemodynamics, pleural pressure and normoxic heliox during exercise in patients with chronic obstructive pulmonary disease
Authors: Bart Boerrigter, Harm-Jan Bogaard, Herman Groepenhoff, Nico Westerhof, Anton Vonk-Noordegraaf
Journal: European Respiratory Journal (2012)

Background . Altered pulmonary mechanics, due to airway obstruction, are thought to hamper cardiac function during exercise in patients with COPD. If so, improving airflow would lead to an enhanced cardiac function.

Methods. Pleural pressure, dynamic hyperinflation and central hemodynamics, by right heart catheterisation, were simultaneously measured in patients with moderate to very severe COPD at rest and during exercise; both in ambient air and while breathing a normoxic helium-oxygen mixture (heliox).

Results. Seventeen patients were included (FEV1: 53 ± 17%, FEV1/VC: 42±10%). Breathing heliox lowered expiratory pleural pressure at rest (3.8±2.6 to 1.8±2.3 mmHg) and during exercise (8.2±3.6 to 6.1±3.3 mmHg), both p<0.05. During exercise we did not find any improvements in stroke volume or cardiac output. At rest we found improvements in cardiac output (6.1±1.4 to 6.6±1.1 L/min/m2), stroke volume (80±22 to 87±19 ml/m2) and mixed venous oxygen saturation (66±6 to 69±6%; all p<0.05). Dynamic hyperinflation did not improve with heliox.

Conclusion. Heliox breathing did not affect cardiac function during exercise. This implies that altered pulmonary mechanics do not substantially affect cardiac function during exercise in COPD.
Chlamydia trachomatis and human papillomavirus infection in Indian women with sexually transmitted diseases and cervical precancerous and cancerous lesions
Authors: V. Gopalkrishna, N. Aggarwal, V.L. Malhotra, R.V. Koranne, V.P. Mohan, A. Mittal, B.C. Das
Journal: Clinical Microbiology and Infection (2000)
978. Optimization of the Tet-on System To Regulate Interleukin 12 Expression in the Liver for the Treatment of Hepatic Tumors
Authors: Maider Zabala, Lin Wang, Ruben Hernandez-Alcoceba, Wolfgang Hillen, Cheng Qian, Jesus Prieto, Maria-Gabriela Kramer
Journal: Molecular Therapy (2004)

Introduction: Intratumor administration of short-term expression vectors encoding interleukin 12 (IL-12) can satisfactory eradicate liver neoplasm in animal models, although little efficacy was shown in pilot clinical trials. Therefore, long-term expression of IL-12 within the liver may be advantageous. Due to toxicity of IL-12, vectors should contain systems for efficient gene regulation. The Tet-on system is composed of a chimeric trans-activator (rtTA2S-M2) and a doxycycline (Dox)-dependent inducible promoter. In this work we pursued: a) the optimization of Tet-on system for tight and liver-specific IL-12 regulation, and b) its application for the treatment of hepatic tumors.
Injury Prevention
Authors: Frederick P. Rivara, David C. Grossman, Peter Cummings
Journal: New England Journal of Medicine (1997)

FallsRates of morbidity and mortality from falls are higher among the elderly than among younger persons. Approximately 60 percent of persons who die from falls are 65 years old or older, and falls account for 87 percent of all fractures in older adults. The most important risk factors for falls and fall-related injuries among the elderly are a history of one or more prior falls,71 cognitive impairment,71 chronic illness,71 balance and gait impairment,71 a low body-mass index,71 female sex,71 general frailty,72 use of diuretics,73 use of psychotropic drugs,74 and hazards in the home.71 Among older adults, hip fracture is . . .
Insertion Site for Central Venous Catheters
Authors: Kleper N. F. de Almeida
Journal: JAMA Internal Medicine (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Internal Medicine HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Internal Medicine journal
Familial Neutrophil Chemotaxis Defect, Recurrent Bacterial Infections, Mucocutaneous Candidiasis, and Hyperimmunoglobulinemia E
Authors: ROBERT E. VAN SCOY
Journal: Annals of Internal Medicine (1975)

A 20-year-old woman and her infant daughter had recurrent bacterial infections and chronic mucocutaneous candidiasis and were found to have extreme hyperimmunoglobulinemia E, defective neutrophil chemotaxis, and diminished lymphocyte responses toCandidaantigen. Studies of members of the mother's family showed mild increases of IgE and mildly depressed chemotactic activity of neutrophils in a brother, the father, and the paternal grandfather. The recurrent bacterial infections in these two patients can be explained by the defective neutrophil chemotaxis. It is not known whether the mucocutaneous candidiasis is related to the neutrophil chemotaxis with the lymphocyte defect being secondary to theCandidainfection or, alternatively, theCandidainfection being secondary to the lymphocyte defect. Furthermore, the family data suggest a familial pattern of hyperimmunoglobulinemia E and defective neutrophil motility.
Coagulation Abnormalities in Fatal Cerebral Lupus Erythematosus<sup>1</sup>
Authors: A Paterson, W Timperley, D Slater
Journal: Journal of the Royal Society of Medicine (1981)

Two cases of fatal systemic lupus erythematosus (SLE) with cerebral involvement are described. Both showed widespread microinfarction of the brain secondary to platelet and fibrin thrombi. There was no evidence of vasculitis. Anticoagulants and antiplatelet drugs may have an important role in the management of cerebral SLE.
The ED and the Uninsured
Authors: Ellen J. Weber, Jonathan A. Showstack, Michael L. Callaham, Kelly A. Hunt, David C. Colby
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Website of the week: Patient power in randomised controlled trials
Authors: C. Martyn
Journal: BMJ (2003)

In his article on the ethics of clinical trials in the BMJ this week (p 980), Richard Lilford argues that patients, not doctors, should decide if there is equipoise between the treatments on offer. Whether or not you agree with him, there is no doubt that providing potential recruits to clinical trials with what they need to make a genuinely informed choice …
Abstract 1637: Difference of Pulmonary Arterial Microvasculature in Patients with Pulmonary Hypertension Using Scanning Electron Microscope
Authors: Aya Miura, Kazufumi Nakamura, Kengo F Kusano, Hiromi Matsubara, Daiji Miura, Ryuji Miura, Aiko Ogawa, Tetsuya Ikeda, Hideki Fujio, Katsumasa Miyaji, Nobuhiro Nishii, Hiroshi Morita, Takefumi Oka, Aiji Ohtsuka, Hiroshi Date, Tohru Ohe
Journal: Circulation (2008)

Background and Objectives: Pulmonary vascular diseases (pulmonary arterial hypertension: PAH, pulmonary veno-occlusive disease: PVOD and pulmonary capillary hemangiomatosis: PCH) are well-known diseases that cause pulmonary hypertension (PH). However, the differences of structural characteristics of pulmonary microvasculature in these diseases have not been elucidated enough. We examined the morphological differences among these diseases by methacrylate resin (Mercox) perfusion using scanning electron microscope. Methods and Results: Ten lung samples were 8 patients with PH and 2 control subjects without PH. These were obtained at lung transplantations or autopsies. Microvascular corrosion replicas were made by Mercox perfusion from the pulmonary artery. Pathological diagnosis revealed 5 PAH, 2 PVOD and 1 PCH in patients with PH. In PAH samples, small arteries or arterioles showed twisted and tortuous courses, but such vessels were not observed in samples from control, PVOD and PCH. Binary branching was observed in control, but not in PVOD and PCH. In PVOD and PCH, several branching was commonly observed. Moreover, the loss of capillary network and the disruption of arterioles were more severe in samples from PVOD and PCH patients than in samples from PAH patients. Conclusion: Pulmonary microvascular morphology is seriously different among the type of underlying diseases in PH patients.
Expression of CEACAM1 in pulmonary adenocarcinoma cells and their metastases
Authors: E. Laack, O. Schult-Kronefeld, I. Burkholder, M. Görn, B. Andritzky, C. Kugler, L. Edler, C. Bokemeyer, U. Schumacher, I. Thöm
Journal: Journal of Clinical Oncology (2006)

17071 Background: The cell adhesion molecule CEACAM1 is involved in intercellular adhesion and is expressed in a variety of normal human tissues such as mammary gland, colonic mucosa and prostate. In cases of malignant transformation of these tissues a down regulation or loss of CEACAM1 has been shown. In contrary, CEACAM1 is not expressed in normal lung tissue or melanocytes and it has been demonstrated that a expression in these tissues is associated with the development of metastatic disease. The aim of the present investigation was to analyze a possible association between the expression of CEACAM1 in pulmonary adenocarcinoma cells and their lymph node and hematogenous metastatic cells. Methods: CEACAM1 expression was immunhistochemically evaluated in primary tumor cells, cells in lymph nodes and in distant metastases of 96 patients with metastatic adenocarcinoma of the lung who had undergone surgery between 1999 and 2002. Results: An expression of CEACAM1 has been shown in 78 of 96 primary tumors. We found a significant positive correlation between the CEACAM1 expression on the cells of the primary tumor and the lymph node metastases (p&lt;0.005) and the hematogenous metastases (p&lt;0.05). Conclusion: As shown before, CEACAM1 is not expressed in normal lung tissue but in most primary adenocarcinomas of the lung. We are the first to demonstrate, that its expression is preserved in the lymph node and hematogenous metastases in metastatic disease implicating that its expression is of functional significance of both metastatic sites. No significant financial relationships to disclose.
Cerebral blood flow changes during sodium-lactate-induced panic attacks
Authors: R S Stewart, M D Devous, A J Rush, L Lane, F J Bonte
Journal: American Journal of Psychiatry (1988)

Dynamic single-photon emission computed axial tomography (CAT) with inhaled xenon-133 was used to measure regional cerebral blood flow in 10 drug-free patients with DSM-III-diagnosed panic disorder and in five normal control subjects. All subjects underwent regional cerebral blood flow studies while at rest or during normal saline infusion and during sodium lactate infusion. Six of the 10 patients and none of the control subjects experienced lactate-induced panic attacks. Lactate infusion markedly raised hemispheric blood flow levels in both control subjects and patients who did not panic. Patients who did panic experienced either a minimal increase or a decrease in hemispheric blood flow.
MicroRNA signature predicts survival and relapse in lung cancer
Authors: Hsuan-Yu Chen, Sung-Liang Yu, Gee-Chen Chang, Huei-Wen Chen, Ker-Chau Li, Jeremy J.W. Chen, Pan-Chyr Yang
Journal: Cancer Research (2008)

4432   Abstract  MicroRNAs are a new class of small non-protein-coding RNAs that function in endogenous negative gene-regulation and tumorigenesis. Lung cancer is the most common cause of cancer deaths worldwide. Current clinical-pathological staging methods are inadequate to predict treatment outcome for lung cancer. Using real-time RT-PCR, we obtained microRNA expressions in 112 NSCLC patients, which were divided into the training and testing sets. Using Cox regression and risk-score analysis, we identified a 5-microRNA signature for the prediction of treatment outcome of NSCLC in the training set. This microRNA signature was validated by the testing set and an independent cohort (62 patients from different medical center). Patients with high-risk score of the microRNA signature had poor overall and disease-free survivals compared to the low-risk score patients the results were consistent across the training, testing, and independent dataset. This microRNA signature is an independent predictor of cancer relapse (hazard ratio=3.29, p value=0.016) and overall survival (hazard ratio=10.31, p value=0.002) of NSCLC patients, as well as in the testing dataset and an independent cohort. In conclusion, we identified a 5-microRNA signature that can predict survival in lung cancer patients and it had been validated twice times. This may have clinical implication in the molecular-pathogenesis of cancer, development of new targeted-therapy or selection of high-risk cancer patients for adjuvant chemotherapy.
Relation Between Clinical, Angiographic and Ischemic Findings at Baseline and Ischemia-Related Adverse Outcomes at 1 Year in the Asymptomatic Cardiac Ischemia Pilot Study fn1fn1This study was funded by the National Heart, Lung, and Blood Institute, Cardiac Diseases Branch, Division of Heart and Vascular Diseases, National Institutes of Health, Bethesda, Maryland, by Research Contracts HV-90-07, HV90-08, HV-91-05 to HV-91-14. Study medications and placebo were donated by Zeneca Pharmaceuticals …
Authors: Carl J. Pepine, Barry Sharaf, Thomas C. Andrews, Sandra Forman, Nancy Geller, Genell Knatterud, John Mahmarian, Pamela Ouyang, William J. Rogers, George Sopko, Richard Steingart, Peter H. Stone, C.Richard Conti
Journal: Journal of the American College of Cardiology (1997)
DE MINIMIS CURAT LEX.
Authors: Brian G Driscoll
Journal: JAMA (1899)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal