CAR T Cells for Acute Myeloid Leukemia: The LeY of the Land
Authors: Malcolm K Brenner
Journal: Molecular Therapy (2013)

Recent studies of CD19-directed chimeric antigen receptor (CAR)-expressing T cells have shown dramatic results in the treatment of acute lymphoblastic leukemia as well as activity in B-cell lymphoma.1Brentjens RJ Davila ML Riviere I Park J Wang X Cowell LG et al.CD19-Targeted T cells rapidly induce molecular remissions in adults with chemotherapy-refractory acute lymphoblastic leukemia.Sci Transl Med. 2013; 5: 177ra38Crossref PubMed Scopus (1498) Google Scholar,2Grupp SA Kalos M Barrett D Aplenc R Porter DL Rheingold SR et al.Chimeric antigen receptor–modified T cells for acute lymphoid leukemia.N Engl J Med. 2013; 368: 1509-1518Crossref PubMed Scopus (2489) Google Scholar,3Kochenderfer JN Dudley ME Feldman SA Wilson WH Spaner DE Maric I et al.B-cell depletion and remissions of malignancy along with cytokine-associated toxicity in a clinical trial of anti-CD19 chimeric-antigen-receptor-transduced T cells.Blood. 2012; 119: 2709-2720Crossref PubMed Scopus (1141) Google Scholar These highly publicized studies have led to the hope that CAR T cells can be used to treat other hematological malignancies and solid tumors as well. The article by Ritchie et al. in this issue4Ritchie DS Neeson PJ Khot A Peinert S Tai T Tainton K et al.Persistence and efficacy of second generation CAR T cell against the LeY antigen in acute myeloid leukemia.Mol Ther. 1999; 21: 2122-2129Abstract Full Text Full Text PDF Scopus (313) Google Scholar shows how the approach can be adapted for the treatment of patients with acute myeloid leukemia (AML) and illustrates that significant obstacles remain before this biological therapy will become a mainstay of treatment. Although CARs directed to the CD19 antigen on lymphoid malignancies are proving to be highly active, there has been concern that it may be difficult to extend this success to many other tumors. The CD19 antigen is restricted to the B-cell lineage in general; it is not limited to the malignant clone. Eliminating a CD19+ malignancy therefore also leads to the eradication of normal B cells. There are few other examples of normal cell lineages that can be safely eliminated along with malignant cells that share the targeted antigen. In particular, targeting an antigen on AML cells that is also present on the normal myeloid lineage would lead to profound neutro­penia, which would have devastating effects on a patient’s health even if his or her AML had been eradicated. Investigators have therefore long sought antigens that are either highly overexpressed on myeloid malignancies compared with normal cells or unique to the malignant myeloid clone. Several such antigens have been identified, but the great majority are internal proteins that are processed and then presented as peptides by the cells’ human leukocyte antigens.5Doubrovina E Carpenter T Pankov D Selvakumar A Hasan A O'Reilly RJ Mapping of novel peptides of WT-1 and presenting HLA alleles that induce epitope-specific HLA-restricted T cells with cytotoxic activity against WT-1(+) leukemias.Blood. 2012; 120: 1633-1646Crossref PubMed Scopus (48) Google Scholar Such peptides can usually be recognized only by the native T-cell receptor, not by an antibody-derived CAR, although monoclonal antibodies that recognize a major histocompatibility complex (MHC)-peptide complex have recently been synthesized.6Dao T Yan S Veomett N Pankov D Zhou L Korontsvit T et al.Targeting the intracellular WT1 oncogene product with a therapeutic human antibody.Sci Transl Med. 2013; 5: 176ra33Crossref PubMed Scopus (132) Google Scholar,7Sergeeva A Alatrash G He H Ruisaard K Lu S Wygant J et al.An anti-PR1/HLA-A2 T-cell receptor-like antibody mediates complement-dependent cytotoxicity against acute myeloid leukemia progenitor cells.Blood. 2011; 117: 4262-4272Crossref PubMed Scopus (98) Google Scholar Ritchie et al.4Ritchie DS Neeson PJ Khot A Peinert S Tai T Tainton K et al.Persistence and efficacy of second generation CAR T cell against the LeY antigen in acute myeloid leukemia.Mol Ther. 1999; 21: 2122-2129Abstract Full Text Full Text PDF Scopus (313) Google Scholar therefore took advantage of one of the defining characteristics of antibody derived CARs, which is that they are MHC-unrestricted and can recognize nonprotein antigens. They developed a CAR that targeted LeY, a difucosylated carbohydrate antigen that is overexpressed by malignant myeloid cells. Following treatment with cytotoxic drugs, they infused T cells expressing this CAR into five patients with relapsed AML, four of whom were evaluable. Modest CAR T-cell expansion and persistence was seen, and two of the four patients had a reduction in their residual disease, although all ultimately relapsed. Although the clinical responses in this study were limited, the results have important implications. The data provide a clinical proof of concept for using CARs to target nonprotein tumor–associated antigens in hematological malignancy, as in solid tumors.8Pule MA Savoldo B Myers GD Rossig C Russell HV Dotti G et al.Virus-specific T cells engineered to coexpress tumor-specific receptors: persistence and antitumor activity in individuals with neuroblastoma.Nat Med. 2008; 14: 1264-1270Crossref PubMed Scopus (920) Google Scholar The data also demonstrate good tolerance of modest levels of CAR T-cell expansion and persistence, although one patient was reported to have neutropenia as an adverse effect. The LeY antigen was not measurably downregulated following CAR T-cell infusion, suggesting that it may indeed serve as a suitable target for long-term immune control of disease. Most important, perhaps, the article demonstrates that the CAR T cells can traffic to the bone marrow and also to disease sites in skin, raising the possibility that the approach may ultimately eliminate both central (e.g., marrow) and peripheral (e.g., skin) reservoirs of disease. Almost all first-in-human proof-of-concept studies raise as many issues as they address, and the work described in the current article is no exception. It is clear that the degree of in vivo expansion of the CAR T cells and the level at which they persist in vivo are likely to be below those required for effective and sustained control of AML. There are many modifications to the CARs themselves that may improve expansion, persistence, and antitumor function in vivo, including the use of alternative costimulatory endodomains and alterations in extracellular spacer and transmembrane sequences.9Ramos CA Dotti G Chimeric antigen receptor (CAR)-engineered lymphocytes for cancer therapy.Expert Opin Biol Ther. 2011; 11: 855-873Crossref PubMed Scopus (112) Google Scholar The procedures for growing the CAR-transduced T cells can also have profound effects on their in vivo performance, not least by selecting subsets that have little long-term engraftment potential or that are “exhausted” and subject to antigen-induced cell death.9Ramos CA Dotti G Chimeric antigen receptor (CAR)-engineered lymphocytes for cancer therapy.Expert Opin Biol Ther. 2011; 11: 855-873Crossref PubMed Scopus (112) Google Scholar In addition, a more sensitive means of tracking transgene expression in vivo will need to be developed for this CAR. The authors were able to follow the CAR T cells only by detecting a transgene-derived PCR signal, which does not by itself show that the transduced T cells express the CAR and can still recognize their intended target. Finally, the CAR T cells may need to be further engineered to resist tumor-immune evasion strategies10Bonini C Brenner MK Heslop HE Morgan RA Genetic modification of T cells.Biol Blood Marrow Transplant. 2011; 17 (1 suppl): S15-S20Abstract Full Text Full Text PDF PubMed Scopus (29) Google Scholar or to be administered with antibodies that serve as checkpoint inhibitors and prevent CAR T-cell inactivation.11Riley JL Combination checkpoint blockade—taking melanoma immunotherapy to the next level.N Engl J Med. 2013; 369: 187-189Crossref PubMed Scopus (60) Google Scholar All these components will need to be optimized before we will be able to accurately evaluate the benefits of the approach. Optimization of this therapy may, of course, reveal new problems. Rapid and substantial in vivo expansion may lead to a systemic inflammatory response syndrome or cytokine storms encountered with other more effective CART-cell therapies,2Grupp SA Kalos M Barrett D Aplenc R Porter DL Rheingold SR et al.Chimeric antigen receptor–modified T cells for acute lymphoid leukemia.N Engl J Med. 2013; 368: 1509-1518Crossref PubMed Scopus (2489) Google Scholar and which were notably absent in the current series. Once the CAR T cells are present at high levels for long periods, we may learn that selective overexpression of LeY is not quite selective enough and that critical normal tissues, such as hemopoietic stem cells, are also damaged. The availability of effective safety or suicide systems may help address this last concern.12Di Stasi A. Tey SK Dotti G Fujita Y Kennedy-Nasser A Martinez C et al.Inducible apoptosis as a safety switch for adoptive cell therapy.N Engl J Med. 2011; 365: 1673-1683Crossref PubMed Scopus (1040) Google Scholar Despite the above limitations, this important clinical proof-of-concept study has shown that CAR T cells can target a nonprotein target antigen in patients with myeloid malignancy; further optimization and extension to other LeY+ tumors may well lead to more striking clinical benefits