tbuckley/cabot-search huggingface dataset:
Heterogeneous transmural gene expression of calcium-handling proteins and natriuretic peptides in the failing human heart
Authors: J Prestle
Journal: Cardiovascular Research (1999)

Objective: Human heart failure is associated with a disturbed intracellular calcium (Ca2+) homeostasis. In this regard, ventricular wall stress is considered to be a determinant for expression of sarcoplasmic reticulum Ca2+-ATPase (SERCA2a). In the present study, we analyzed the transmural protein and/or mRNA levels of SERCA2a, other Ca2+-handling proteins, and of atrial and brain natriuretic peptides (ANP and BNP) in the human heart. Methods: Subepicardial (epi), midmyocardial (mid), and subendocardial (endo) sections of the left ventricular free wall from end-stage failing (n=17) and nonfailing (n=5) human hearts were analyzed by Western blot for immunoreactive protein levels of SERCA2a, phospholamban (PLN), and calsequestrin (CS). Subepi- and subendocardial sections were analyzed by Northern blot for steady-state mRNA levels of SERCA2a, Na+-Ca2+ exchanger (NCX1), ANP, and BNP. Results: SERCA2a protein and mRNA levels were reduced by 40±5% (P<0.01) and 25±7% (P<0.05) in endo compared to epi in the failing heart and by 27±14% and 16±12% (non-significant) in the nonfailing heart, respectively. PLN protein levels were reduced by 23±6% (P<0.05) in endo compared to epi in the failing heart and by 17±25% (non-significant) in the nonfailing heart, whereas CS protein levels and NCX1 mRNA levels were similar across the left ventricular wall. Strikingly, in the failing heart, both BNP and ANP mRNA levels were upregulated predominantly in endo. Conclusions: In the failing human heart, SERCA2a and PLN, as well as natriuretic peptides but not CS and NCX1 are differentially expressed across the left ventricular wall, implicating (1) different susceptibility of subendocardium and subepicardium to factors affecting expression of these proteins and (2) differences in regulation of the distinct calcium-cycling proteins.
Serum Immunoglobulin Levels in Blood Donors Implicated in Transmission of Hepatitis
Authors: Gerald Bevan
Journal: JAMA (1968)

The increased serum immunoglobulin level that occurs in viral hepatitis may persist for at least one to two years after the acute attack. Similar changes have been found in 21 of the expected maximum of 36 carriers of hepatitis virus among 63 blood donors implicated in the transmission of hepatitis. Immunoglobulin assay may provide a useful screening procedure for potential blood donors.
Personal View: An Anthology
Authors: Harry B. Greenberg
Journal: JAMA (1976)

Should a respectable man be imprisoned for smacking the bottoms of willing and erotically excited girls? "Ridiculous," writes an orthopedic surgeon in one of the short essays that make up<i>Personal View</i>. Sex, he observes, is for delight. Or, a professor notes the shifting of capital from men in their 50s who die from their exertions, to their wives, who survive them by 15,20, and 30 years, and concludes: "Blessed are those with two X chromosomes, for they shall inherit the earth." Written by doctors, nurses, and medical workers, as their fancies dictate, the 100 three-page essays in<i>Personal View</i>beggar description. A medical meeting leads the Birmingham consultant to wonder if the traditional marathon of sitting—or sleeping—in soporific gloom through a series of 15-minute papers is the best way for promoting new ideas. A London sister laments the absence of nursing by trained staff outside an intensive care unit;
Abstract 414: Endothelial dysfunction of Epicardial Resistance Arteries in Pigs after Coronary Artery Implant of Paclitaxel-Eluting Stents
Authors: Jinsheng Li, Lakshmana Pendyala, Toshiro Shinke, Jack P Chen, Nicolas Chronos, Keith Robinson, Dongming Hou
Journal: Circulation (2008)

Background: Abnormal endothelium-dependent vasorelaxation of coronary arteries adjacent to drug-eluting stent (DES) implants has been reported and may be associated with late stent thrombosis. However, epicardial resistance vessel vasomotor function in the distal perfusion bed of DES has not been studied. We tested small vessel function 1 mo after coronary artery paclitaxel-eluting stent (PES) implant. Methods: Pigs (n=9) received overlapping bare metal stent (BMS) and PES as well as naïve vessels. One month later, myocardium distal to the stented region, as well as similar location in non-stented vessel (nSV) was harvested. Eighteen epicardial resistance arteries (lumen diameter ~ 250μm) were isolated under a dissecting microscope and mounted on a myograph apparatus. Vasoconstriction (KCl, PGF2α &amp; ET-1), endothelial-dependent relaxation (EDdR) response to substance P (SP), and endothelial-independent relaxation (EDiR) to sodium nitroprosside (SNP) were measured. Results: Angiographic late lumen loss at 1-mo was less for PES than BMS (p=0.002). Epicardial resistance artery relaxation was attenuated in the perfusion distribution of PES, as maximal EDdR in response to SP was 56.0±7.0% compared to vessels isolated from the perfusion distribution of BMS (85.0±7.0%) and non-stented coronary segments (107±2.0%, P=0.034 vs. PES). Maximal EDiR in response to SNP, however, was similar among the groups (90.0±6.0%, PES; 98.0±2.0%, BMS; 123±5.0%, nSV; P=NS respectively). Similarly contraction to endothelin-1 was not affected by stent type (3.95±0.56%, PES; 3.07±0.65%, BMS; 3.01±0.36%, nSV; ratio of contraction ET-1/KCl; P=NS respectively). Conclusions: Epicardial resistance artery endothelial-dependent vasomotor dysfunction is present one month after overlapping coronary PES implant in swine. Derangement of microcirculatory vasomotor function may also be present with DES implant in humans and be associated with a spectrum of adverse clinical sequelae.
“Cold” Reflex Sympathetic Dystrophy in an Adult
Authors: SOPHIE TURPIN, RAYMOND TAILLEFER, RAYMOND LAMBERT, JEAN LEVEILLE
Journal: Clinical Nuclear Medicine (1996)

The classical presentation of acute reflex sympathetic dystrophy (RSD) on triple-phase bone scintigraphy usually consists of increased periarticular uptake in each phase. The authors present a rare case of acute adult RSD characterized by bone hypofixation of Tc-99m MDP. Reflex sympathetic dystrophy in adults and children is reviewed.
Gonadotropin-Releasing Hormone Analogue for Premenopausal Women With Breast Cancer
Authors: Sibylle Loibl
Journal: JAMA (2011)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Changes in plasma urate concentration immediately after acute myocardial infarction.
Authors: P Ghosh, A M Cochrane, P W Gordon, A G Radcliffe
Journal: BMJ (1975)

The plasma urate concentration of 70 patients with acute myocardial infarction increased progressively up to the seventh day and was significantly higher than in a group of 23 patients with ischaemic changes only. Diuretic treatment might have accounted for an early rise in urate levels but the persistent increase seen at seven days could not be so explained.
Succinyl-CoA: 3-Ketoacid CoA-Transferase Deficiency. A CAUSE FOR KETOACIDOSIS IN INFANCY
Authors: J. Tyson Tildon, Marvin Cornblath
Journal: Journal of Clinical Investigation (1972)

To explain the cause of a unique form of severe and intermittent ketoacidosis in an infant who expired after 6 months of life, tissue culture fibroblasts and post mortem tissue were examined for enzyme activities that catalyze glucose and ketoacid oxidation. No measurable succinyl-CoA: 3-ketoacid CoA-transferase (CoA-transferase) activity could be detected in homogenates of the post mortem brain, muscle and kidney tissue, or in the cultured skin fibroblasts. Since seven other enzyme activities involving both glycolysis and ketone body oxidation were present in these same tissues, it was reasonable to conclude that the observed absence of CoA-transferase activity was not an artifact of homogenate preparation. It was concluded that the absence of CoA-transferase activity resulted in a loss of intracellular homeostasis leading to ketoacidosis. In addition, the absence of this enzyme appears to be a reasonable explanation for the alteration in glucose metabolism that was previously reported in fibroblasts from this patient.
Expression of indoleamine 2.3-dioxygenase by tumors induces local and systemic immunosuppressive effects in a murine melanoma model
Authors: Rikke B Holmgaard, Dmitriy Zamarin, David H Munn, Taha Merghoub, Jedd D Wolchok
Journal: Journal for ImmunoTherapy of Cancer (2014)

Meeting abstracts Indoleamine 2,3-dioxygenase (IDO) is found in the majority of human tumors and has been described as an important contributor to the development of an immunosuppressive tumor microenvironment that blocks the action of cytotoxic antitumor effector T cells. In order to delineate the
Indeterminate fulminant hepatitis: Clinico-pathological, immunological and virological reevaluation of the pre and post transplantation period of 31 cases transplanted since 1987
Authors: E. Kimmoun, A.M. Roque-Afonso, C. Johanet, P. Ichai, F. Saliba, B. Roche, R. Adam, D. Azoulay, D. Castaing, F. Yilmaz, M. Gigou, C. Guettier, E. Dussaix, H. Bismuth, D. Samuel, J.C. Duclos-Vallee
Journal: Journal of Hepatology (2003)
1082-180 Compensatory enlargement delay vascular healing of the culprit lesion following acute myocardial infarction
Authors: Takahiro Hayashi, Takashi Kiyoshima, Masayoshi Matsuura, Naoya Kobayashi, Masafumi Ueno, Norihiro Takeda, Hajime Nakamura, Hiroshi Yabushita, Atsuhiro Kurooka, Mitsugu Taniguchi, Masaru Miyataka, Akio Kimura, Kinji Ishikawa
Journal: Journal of the American College of Cardiology (2004)
Abstract 1242: Green tea polyphenols inhibit the growth and induce death of melanoma cells by initiating DNA damage and inhibition of class I histone deacetylases
Authors: Ram Prasad, Santosh K. Katiyar
Journal: Cancer Research (2014)

Abstract Melanoma remains a leading cause of death from skin diseases due to its propensity to metastasis, and is increasing rapidly in children. Although, melanoma is less common than other types of skin cancers, however, it causes the majority (75%) of skin cancer-related deaths. The average survival of patients with advanced stage of melanoma is less than a year because no effective strategies are available for metastatic melanoma. Since melanoma is a highly malignant cancer, an approach that can inhibit its growth and progression may facilitate the development of an effective strategy for its treatment or prevention. As green tea polyphenols (GTPs) have been shown to have anti-skin carcinogenic effects, we have examined the chemotherapeutic effect of GTPs on the growth of different melanoma cell lines and the molecular mechanisms underlying these effects using an in vitro model. Treatment of melanoma cells (A375 and Hs294t) with GTPs (0, 20, 40 and 60 µg/ml) for 24 and 48 h significantly inhibited histone deacetylase (HDAC) activity, reduced the levels of class 1 HDAC proteins (HDAC 1, 2, 3 and 8) and enhanced histone acetyltransferase (HAT) activity in a dose-dependent manner. These effects of GTPs on melanoma cells were associated with DNA damage, which was evident by Comet assay, and significant reduction in the viability of melanoma cells and induction of cell death. Concomitant treatment of melanoma cells with a proteasome inhibitor, MG132, prevented GTPs-induced degradation of class I HDACs, suggesting that GTPs reduced the levels of HDAC proteins in melanoma cells through proteasomal degradation. Valproic acid, an inhibitor of HDACs, exhibited a similar pattern of reduced viability and induction of death of melanoma cells (A375 and Hs294t). Treatment of A375 and Hs294t cells with GTPs resulted in an alteration of cell cycle regulatory proteins of G0/G1 phase, and that is a decrease in the levels of cyclin D1, D2 and E, and cyclin dependent kinases, CDK2, CDK4 and CDK6 in a dose-dependent manner. Simultaneously, it was also observed that the levels of p21 and p53 proteins were also increased in the cells after the treatment with GTPs, which may have a role in G0/G1 phase arrest and DNA damage. Together, our study provides new insights into the role of class I HDACs in the chemotherapeutic effects of green tea polyphenols on melanoma cells. Citation Format: Ram Prasad, Santosh K. Katiyar. Green tea polyphenols inhibit the growth and induce death of melanoma cells by initiating DNA damage and inhibition of class I histone deacetylases. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1242. doi:10.1158/1538-7445.AM2014-1242
Presentation of Diagnostic Test Accuracy
Authors: Daniel J. Brotman
Journal: Annals of Internal Medicine (2006)

Letters7 February 2006Presentation of Diagnostic Test AccuracyDaniel J. Brotman, MDDaniel J. Brotman, MDFrom Johns Hopkins University Hospital, Baltimore, MD 21287.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-144-3-200602070-00020 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:Puhan and colleagues (1) deserve praise for their creative assessment of how well physicians interpret diagnostic test results, but I disagree with their conclusion that likelihood ratios are no more informative than sensitivity and specificity. The authors asked clinicians to estimate the post-test probabilities of various conditions by using pretest probabilities and diagnostic test results. The operating characteristics of each diagnostic test were provided in terms of likelihood ratios, sensitivity and specificity, or a graphic display. There are 2 problems with this approach.First, familiarity with the method is required. When I finished medical school in the ...References1. Puhan MA, Steurer J, Bachmann LM, ter Riet G. A randomized trial of ways to describe test accuracy: the effect on physicians' post-test probability estimates. Ann Intern Med. 2005;143:184-9. [PMID: 16061916] LinkGoogle Scholar2. Brotman DJ, Segal JB, Jani JT, Petty BG, Kickler TS. Limitations of D-dimer testing in unselected inpatients with suspected venous thromboembolism. Am J Med. 2003;114:276-82. [PMID: 12681454] CrossrefMedlineGoogle Scholar Author, Article, and Disclosure InformationAffiliations: From Johns Hopkins University Hospital, Baltimore, MD 21287.Disclosures: None disclosed. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoA Randomized Trial of Ways To Describe Test Accuracy: The Effect on Physicians' Post-Test Probability Estimates Milo A. Puhan , Johann Steurer , Lucas M. Bachmann , and Gerben ter Riet Presentation of Diagnostic Test Accuracy Milo A. Puhan , Johann Steurer , and Gerben ter Riet Presentation of Diagnostic Test Accuracy Mike Broce and Bernardo Reyes Metrics 7 February 2006Volume 144, Issue 3Page: 221KeywordsConflicts of interestErectile dysfunctionLaboratory testsMyocardial infarctionSpecificity ePublished: 7 February 2006 Issue Published: 7 February 2006 Copyright & PermissionsCopyright © 2006 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
OXYGEN TREATMENT OF MALIGNANT EDEMA
Authors: R. P. Roantree
Journal: JAMA (1926)

In reviewing the literature on this subject, I can find no mention of a patient treated in this manner or a description of a simple apparatus that may be used in injecting the oxygen. <h3>REPORT OF CASE</h3> P. J., a muscular man, aged 26, entered Elko General Hospital, Sept, 6, 1924, complaining of a revolver wound of the left elbow. His past and family history are irrelevant. The injury occurred between 9 and 10 p. m., Sept. 3. The arm was cleansed and the wound irrigated with compound solution of cresol. The bullet had been removed from beneath the skin at about the middle of the mesial side of the arm by a layman. The wound was irrigated with compound solution of cresol twice on the 4th and three times, September 5. The pulse rate was 85, the temperature, 99.6 F., and respirations, 22. There was a bullet wound in
Effects of Adrenomedullin Inhalation on Hemodynamics and Exercise Capacity in Patients With Idiopathic Pulmonary Arterial Hypertension
Authors: Noritoshi Nagaya, Shingo Kyotani, Masaaki Uematsu, Kazuyuki Ueno, Hideo Oya, Norifumi Nakanishi, Mikiyasu Shirai, Hidezo Mori, Kunio Miyatake, Kenji Kangawa
Journal: Circulation (2004)

Adrenomedullin (AM) is a potent pulmonary vasodilator peptide. However, whether intratracheal delivery of aerosolized AM has beneficial effects in patients with idiopathic pulmonary arterial hypertension remains unknown. Accordingly, we investigated the effects of AM inhalation on pulmonary hemodynamics and exercise capacity in patients with idiopathic pulmonary arterial hypertension.Acute hemodynamic responses to inhalation of aerosolized AM (10 microg/kg body wt) were examined in 11 patients with idiopathic pulmonary arterial hypertension during cardiac catheterization. Cardiopulmonary exercise testing was performed immediately after inhalation of aerosolized AM or placebo. The work rate was increased by 15 W/min until the symptom-limited maximum, with breath-by-breath gas analysis. Inhalation of AM produced a 13% decrease in mean pulmonary arterial pressure (54+/-3 to 47+/-3 mm Hg, P<0.05) and a 22% decrease in pulmonary vascular resistance (12.6+/-1.5 to 9.8+/-1.3 Wood units, P<0.05). However, neither systemic arterial pressure nor heart rate was altered. Inhalation of AM significantly increased peak oxygen consumption during exercise (peak o(2), 14.6+/-0.6 to 15.7+/-0.6 mL. kg(-1). min(-1), P<0.05) and the ratio of change in oxygen uptake to that in work rate (Deltao(2)/DeltaW ratio, 6.3+/-0.4 to 7.0+/-0.5 mL. min(-1). W(-1), P<0.05). These parameters remained unchanged during placebo inhalation.Inhalation of AM may have beneficial effects on pulmonary hemodynamics and exercise capacity in patients with idiopathic pulmonary arterial hypertension.
F-18 2-Fluoro-2-Deoxy-Glucose Positron Emission Tomography/Computed Tomography for the Detection of Radicular and Peripheral Neurolymphomatosis
Authors: Christian von Falck, Thomas Rodt, Steffen Joerdens, Stephan Waldeck, Holger Kiesel, Wolfram H. Knapp, Michael Galanski
Journal: Clinical Nuclear Medicine (2009)

Neurolymphomatosis is an uncommon extranodal manifestation of non-Hodgkin lymphoma. It may mimic a broad variety of neurologic conditions which renders clinical diagnosis challenging. As blind nerve biopsy is invasive and may be false negative, surrogate criteria for the diagnosis of neurolymphomatosis have been proposed based on magnetic resonance imaging/computed tomography findings. However, these morphologic modalities may suffer from limited sensitivity. Recently, a few reports have been published that discuss a possible advantage of F-18 2-fluoro-2-deoxy-glucose positron emission tomography/computed tomography (F-18 FDG PET/CT) in these patients.We report the case of a 41-year-old man who presented with progressive tetraparesis and dysaesthesia, in which F-18 FDG PET/CT aided to the diagnosis of neurolymphomatosis due to a large B-cell lymphoma. The patient received chemotherapy (R-CHOP) and the neurologic symptoms were clearly regressive. Three months after the end of systemic chemotherapy the patient presented again with progressive neurologic symptoms. A second PET/CT was performed and demonstrated disease recurrence in the right testis as well as widespread neurolymphomatosis. Additional ultrasound and magnetic resonance imaging examinations were performed and confirmed infiltration of the left brachial plexus, the right femoral, and the right sciatic nerve.We present this case to support the hypothesis that F-18 FDG PET/CT is a valuable imaging modality in patients with suspected neurolymphomatosis. It allows one to accurately determine the extent of the disease in a single whole-body examination.
Coarctation of the aorta and baroreceptor resetting. A study of carotid baroreceptor stimulus-response characteristics before and after surgical repair in the dog.
Authors: F O Igler, L E Boerboom, P H Werner, J H Donegan, E J Zuperku, L I Bonchek, J P Kampine
Journal: Circulation Research (1981)

We studied baroreceptor function in dogs before and after surgical repair of coarctation of the aorta by direct recording of multifiber carotid sinus (CS) nerve activity (NA) during alteration of pulsatile arterial pressure with systemic phenylephrine and nitroprusside, and during static pressure changes using a CS pouch preparation. Coarctation was induced by banding the proximal thoracic aorta in ten 3- to 5-day old puppies. One and one-half years later, five of these coarctated animals were studied before, and five were studied 3-7 months after, surgical repair. Five adult animals also were studied 4-6 months after the proximal thoracic aorta had been banded. Controls were eight normal adult dogs. Threshold pressure at which NA began, saturation pressure at which NA reached a maximum, and slope (% Max NA/mm Hg) of the linear portion of the stimulus-response curve were determined. Pulsatile manipulations of pressure elicited normal sensitivity (slope) in dogs with coarctation but static nonpulsatile pressure changes showed depressed sensitivity compared to controls. After surgical repair, threshold and saturation returned toward normal; sensitivity determined with static pressure manipulations returned to control value. Coarctation reset CS baroreceptors to operate at higher pressures in both puppies and adult dogs and repair of coarctation returned function toward normal. We conclude that resolution of hypertension after repair of coarctation may depend upon baroreceptor readaptation.