tbuckley/cabot-search huggingface dataset:
Trends of asthma in adolescents in Novosibirsk
Authors: Elena Kondiurina, Tatiana Elkina, Vera Zelenskaia, Maria Shabliy, Natalia Timinskaia
Journal: European Respiratory Journal (2014)

Background: The latest epidemiological research of adolescents asthma in Novosibirsk was carried out in 2002 due to Three Phase ISAAC.Then such studies in our city were not met.  Aim: To study trends of asthma in children aged 13-14 in Novosibirsk.  Methods: To estimate trends of asthma in adolescents during the period from 2002 to 2012 yrs it was examined 3025 children aged 13-14 yrs in 2012 by standardized questionnaire ISAAC. Among them were49,9% boys. These data were compared with those obtained in 2002.  Results: This study was showed that the number of adolescents reported to have had asthma increased by 0,12% per year (from 3,8% to 6,6%, p   0,05). Severe wheeze limiting speech were also recorded a little less often – from 1,7% in 2002 to 1,2% in 2012 (p > 0,05).  Conclusions: The study showed that significantly has improved diagnosis of asthma in adolescents in Novosibirsk. However, for other asthma symptoms positive trends are observed. Has remained stable percentage of children 13-14 years with persistent asthma and with severe asthma.
Multiplexed detection of protein and phosphorylation variation in response to androgen withdrawal in LNCaP cells
Authors: Yi-Mi Wu, Jennifer Lunger, Gilbert Omenn, Brian Haab
Journal: Cancer Research (2007)

AACR Annual Meeting-- Apr 14-18, 2007; Los Angeles, CA

2825 

The ability to measure protein concentrations and activation states from multiple proteins in a very low volume, multiplexed format would have valuable applications in cancer studies. Here we report the development of an antibody-array platform for the parallel measurement of the abundances and phosphorylation states of numerous signaling proteins using <2 µl of whole-cell extract per assay. With two-to-four antibodies per targeted protein, sandwich assays were developed and tested efficiently for MAP kinases, AKT, GSK3B, SHIP, androgen receptor (AR), Smad family proteins, STAT5, TGFb, and HIF1A in a study of androgen-related signaling. In addition, the phosphorylation levels of those proteins were assessed using either pan-specific phospho-serine, phospho-threonine, or phospho-tyrosine antibodies, or antibodies against specific protein phosphorylation sites. Various slide blocking reagents, cell lysis buffers, and cell lysate concentrations were tested to optimize the specificity and sensitivity of the assay. Changes in the protein levels and phosphorylation states of the targeted proteins were examined in response to androgen withdrawal and di-hydrotestosterone re-stimulation of LNCaP, an androgen-dependent prostate cancer cell line. AR and Smad4 protein levels were steadily repressed after androgen withdrawal (using charcoal/dextran-treated serum), relative to control cells grown in normal serum, and AR protein levels but not Smad4 levels were restored after androgen re-stimulation. AKT protein levels dropped after androgen withdrawal and rose after about nine days, but its phospho-protein levels initially rose, then dropped (in replicate assays). Other proteins showed less dramatic but consistent androgen-related variation. These results give preliminary insights into different pathway responses to androgen withdrawal and re-stimulation and a possible shift from cell-survival activation after extended androgen withdrawal. These results also demonstrate the usefulness of miniaturized, multiplexed methods for measuring both protein and phosphorylation levels in parallel immuno-assays. [Supported by MTTC GR687 and U54DA021519]
Abstract 11090: Atorvastatin Reduces Uptakes of F-18 Fluorodeoxyglucose on Positron Emission Tomography Accumulation in Atherosclerotic Plaques
Authors: Hideki Ishii, Masami Nishio, Hiroshi Takahashi, Toru Aoyama, Miho Tanaka, Takanobu Toriyama, Tsuneo Tamaki, Tetsuya Amano, Tatsuaki Matsubara, Toyoaki Murohara
Journal: Circulation (2010)

Objectives: Vascular inflammatory status would be an important factor to know plaque vulnerability. Recent studies have shown that F-18 fluorodeoxyglucose (18F-FDG) on positron emission tomography ...
Review article: From allergen genes to new forms of allergy diagnosis and treatment
Authors: S. Vrtala
Journal: Allergy (2008)

Type I allergy represents an important health problem that affects more than 25% of the population in industrialized countries. Specific immunotherapy is one of the few causative treatment approaches for type I allergy and is currently performed with crude allergen extracts, which consist of a mixture of allergenic and nonallergenic components, are difficult to standardize and cannot be applied according to the patient’s reactivity profile. With the introduction of molecular biological techniques into allergy research, a large panel of individual recombinant allergens has become available. Recombinant allergens can be used for improved diagnosis of allergy to determine the patient’s sensitization profile, which is a prerequisite to select the allergens for patient‐tailored immunotherapy. They allow the elucidation of the properties of allergens and of the mechanisms of allergy as well as of the mechanisms of immunotherapy. Moreover, recombinant allergens allow the development of hypoallergenic allergen derivatives with reduced allergenic activity and retained immunogenicity. First immunotherapy trials with hypoallergenic allergen derivatives have shown that this treatment might improve immunotherapy in the near future. This review summarizes the results, which were obtained with recombinant allergens and hypoallergenic allergen derivatives. The experiences from the in vitro and in vivo evaluation of the hypoallergenic derivatives and from clinical studies as well as the contribution of hypoallergenic derivatives to develop new treatment strategies and possibly prophylactic vaccination strategies are discussed.
Abstract 1842: Synergistic inhibition of ALK inhibitor-resistant lung cancer cells by dual-targeting <i>EGFR</i> and <i>ALK</i> pathways
Authors: Soyeon Kim, Tae Min Kim, Yong-Oon Ahn, Bhumsuk Keam, Se-Hoon Lee, Dae Seog Heo, Dong-Wan Kim
Journal: Cancer Research (2014)

Abstract Background: Despite the excellent initial responses of ALK-rearranged lung cancer to the ALK tyrosine kinase inhibitors (TKIs), most patients develop acquired resistance through several mechanisms, including ALK amplification, mutation or activation of alternative signaling pathways. In this study, we investigated the effect of dual-targeting EGFR and ALK pathways in NSCLC cells that are resistant to ALK inhibitors. Methods: The cell proliferation, apoptosis and cell cycle analysis were measured by spectrophotometry and flow cytometry. The combination effect of EGFR TKI (afatinib or dacomitinib) and ALK TKI (crizotinib or LDK378) was evaluated using a combination index (CI). We analyzed EGFR ligand levels by ELISA and expression of ALK, EGFR and its downstream molecules were detected by Western blot assay after exposure to TKIs. Results: NCI-H3122CR1 that was resistant to crizotinib and NCI-H3122 cell lines became resistant to LDK378 through the step-wise increase of the drug, resulting in H3122CR1LR1 and H3122LR1 cells, respectively. The resistant cell lines exhibited 10-14-fold higher IC50 values for crizotinib or LDK378 compared with parental cells. The genetic alterations of ALK or EGFR genes were not found in H3122CR1, H3122LR1, and H3122CR1LR1 cells. We analyzed EGFR signaling pathways in these cells to determine their roles in mediating ALK inhibitor resistance. We observed that H3122CR1, H3122LR1 and H3122CR1LR1 cells had higher phospho-EGFR than parental cells. In these cells, mRNA and protein levels of amphiregulin and EGF were up-regulated. Combined treatment with EGFR TKI and LDK378 synergistically inhibited cell growth (CI&amp;lt;1) and induced apoptosis in H3122LR1 and H3122CR1LR1 cells. Similar results were observed in H3122CR1 and SNU-2535 harboring ALK G1269A mutation cells, when exposed to afatinib and crizotinib. The combination of EGFR and ALK TKIs decreased the phosphorylation of several downstream molecules in H3122LR1 and H3122CR1LR1 cells, whereas EGFR or ALK TKI alone did not reduce the phosphorylation levels. Conclusion: Our results suggest that dual inhibition of ALK and EGFR effectively abrogate ALK and its downstream signals and induce apoptosis in ALK TKIs-resistant, ALK-rearranged NSCLC cells. Therefore, dual-targeting of EGFR and ALK might be a promising strategy for ALK-rearranged NSCLC patients who showed acquired resistance to crizotinib and/or LDK378. Citation Format: Soyeon Kim, Tae Min Kim, Yong-Oon Ahn, Bhumsuk Keam, Se-Hoon Lee, Dae Seog Heo, Dong-Wan Kim. Synergistic inhibition of ALK inhibitor-resistant lung cancer cells by dual-targeting EGFR and ALK pathways. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1842. doi:10.1158/1538-7445.AM2014-1842
LSC 2011 Abstract: The relationship of CD4+/CD8+ ratios and toll like receptor-2 expression with COPD and smoking
Authors: Gurol Sahin Ulutas, Aylin Ozgen Alpaydin, Fatma Taneli, Cemile Cetinkaya, Cevval Ulman, Gonul Dinc, Aysin Sakar Coskun
Journal: European Respiratory Journal (2011)

Aim:  Native immune system activates acquired immunity by Toll Like Receptors (TLR). There may be some alterations in T cell profile and TLR expression in peripheral blood monocytes of COPD patients. We aimed to evaluate the utilization of the ratio of CD4+, CD8+ T cells and TLR-2 expression as a marker of lung pathology as well as their relationship between pulmonary function tests in COPD patients and smokers.   Method:  Forty stable COPD patients admitted to our university9s outpatient clinic and 40 volunteers were included in the study. The study population was evaluated in 4 groups according to their smoking status. CD4+, CD8+ T cells and monocyte TLR-2 expression was measured by flow cytometry in patients and control groups. Spirometry was performed in the whole study population except for nonsmoker control group.   Results:  TLR-2 expressions investigated in CD14+ cells were found%56±25 in Group 1 (nonsmoker COPD),%65±23 in Group 2 (smoker COPD),%64±24 in Group 3 (healthy smoker) and%52±25 in Group 4 (healthy nonsmoker), any difference was not observed between the groups. CD4+, CD8+ T cells and CD4+/CD8+ ratios were not found to be different between the groups. CD4+ T cells and FEV 1 , FEV 1 /FVC had a positive correlation (r=0,311, p=0,01; r=0,293, p=0,023, respectively). CD4+/CD8+ ratios and FEV 1 /FVC also showed positive correlation (r=0,295, p=0,022). Smoking amount and CD4+/CD8+ ratios had a negative correlation (r= -0,274, p=0,034).   Conclusion:  The results of our study demonstrated that CD4+/CD8+ ratio may be used as a biomarker to evaluate the pathogenesis of COPD, however TLR-2 is not convenient for this purpose.
A New Inclusion Body in Rheumatoid Synovia
Authors: T. C. Highton, D. E. Caughey, D. G. Rayns
Journal: Annals of the Rheumatic Diseases (1966)

As part of the current research programme these laboratories are investigating the synovia of normal and abnormal human joints by light and electron microscopy.By synovia is meant the material lining joints and tendon sheaths on the interior of the fibrous capsule, including, therefore, synovial cells, loose connective tissue, blood vessels, and inflam- matory cells which may be present.Several descriptions of the fine structure of normal or near normal human synovium have been published (Barland, Novikoff, and Hamerman, 1962, and Coulter, 1962), and also of the synovium in rheumatoid arthritis (Barland, Novikoff, and Hamerman, 1964).However, none of these has included a study of blood vessels.In view of the importance of the alterations in blood vessels seen on light micro- scopy (Kulka, Bocking, Ropes and Bauer, 1955), it was thought worth while to pay these particular attention during this ultrastructural study, and it is the purpose of this paper to draw attention to some endothelial inclusion bodies occurring within, or immediately adjacent to, small synovial blood vessels in rheumatoid arthritis which we think sufficiently interesting to merit further investigation.
Dutch doctors reject government advice on HIV testing in pregnant asylum seekers
Authors: Tony Sheldon
Journal: BMJ (2000)

Dutch doctors have rejected the advice of their chief inspector of health care that, before automatically offering pregnant asylum seekers or illegal immigrants an HIV test, they should first consider their patients' chances of being repatriated.

Writing in the Royal Dutch Medical Association's journal, Medisch Contact , gynaecologist Dr Jules Schagen van Leeuwen argues that the advice seriously violates trust in the medical profession.

“Anybody who comes with a legitimate request for help must do so in the knowledge that they will receive the best possible care.” This includes …
Abstract 17064: Frailty Predicts Mortality and Hospital Readmission Amongst Elderly Heart Failure (HF) Patients Discharged From Hospital or the Emergency Department (ED)
Authors: Penelope Rampersad, Susan E Bronskill, Alice Chong, Peter C Austin, Jack V Tu, Douglas S Lee
Journal: Circulation (2014)

Introduction: Frailty is commonly encountered in elderly patients with HF. However, the impact of both frailty and HF on outcomes in the population has not been delineated. There is a need for operationalizable definitions of frailty to explore variations in care among the elderly. Objective: To assess common indicators of frailty as predictors of mortality and rehospitalisation within 1 year in a population-based study of very elderly HF patients. Methods: Community-dwelling HF patients aged &gt;75 yrs discharged from hospital or ED in Ontario, Canada, were studied using the Enhanced Feedback for Effective Cardiac Treatment and Emergency HF Mortality Risk Grade databases. Surrogates of frailty, including dementia, falls, incontinence, immobility, caregiver dependence, and abnormal weight loss, were identified from chart abstraction and linked hospitalization records. Survival analyses were performed using multiple Cox regression, for frail vs non-frail status and for each frailty component. Results: 9964 patients were identified (age 84±5 yrs). After adjustment for age, sex, vital signs, laboratory variables, and comorbidities, presence of any frailty indicator was associated with mortality: adjusted hazard ratio [HR] 1.18 (95%CI; 1.06-1.32, p=0.003). Frailty increased the risk of readmission and the association was more pronounced than for mortality (see Figure ): adjusted HR 1.29 (95%CI; 1.18-1.41, p&lt;.001). Among the individual frailty components, significant predictors of mortality were: dementia (HR 1.49, 95%CI; 1.34-1.64, p&lt;.001) and care provider dependency (HR 1.17, 95%CI; 1.01-1.36, p=0.038). In contrast, other frailty components increased the risk of readmission, specifically: falls (HR 1.17, 95%CI; 1.06-1.29, p=0.003) and incontinence (HR 1.64, 95%CI; 1.18-2.29, p=0.004). Conclusion: Frailty indicators derived from clinical and administrative data sources are associated with death and readmission in a very elderly HF cohort.
Childhood obesity
Authors: John J Reilly, David Wilson
Journal: BMJ (2006)

Obesity, an excessive body fat content with increased risk of morbidity, has become increasingly common in children and adolescents. Confusion exists, however, over basic questions such as whether paediatric obesity matters, how to diagnose it, and whether it should be treated (and if so, how best to do this). Doctors in many fields need a better understanding of these issues.
Review of Andrew Thompson and Norman Temple, eds., 2001.<i>Ethics, Medical Research, and Medicine: Commercialism versus Environmentalism and Social Justice</i>
Authors: Kathleen Nolan
Journal: The American Journal of Bioethics (2003)

"Review of Andrew Thompson and Norman Temple, eds., 2001. Ethics, Medical Research, and Medicine: Commercialism versus Environmentalism and Social Justice ." , 3(2), pp. 69–70
Surge in Global Cancer Rates?
Authors: Joan Stephenson
Journal: JAMA (2003)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Norovirus outbreaks on three college campuses - California, Michigan, and Wisconsin, 2008.
Authors: C. M. Roberts, J. Archer, T. Renner, P. A. Heidel, D. L. VandeBunte, B. M. Brennan, C. Croker, R. Reporeter, S. Nakagawa-Ota, A. J. Hall
Journal: MMWR Morbidity and Mortality Weekly Report (2009)
Abstract 1580: Age-related difference in the susceptibility phthalate-induced injury in Sprague-Dawley rat
Authors: Kassim Traore
Journal: Cancer Research (2014)

Abstract Di-2-ethylhexyl phthalate (DEHP), a commonly used plasticizer of synthetic polymers causes liver tumorigenesis, and both short and long-term effects on the reproductive system of male rats. Whether initial exposures to DEHP in early developmental stage affect the susceptibility to injury by subsequent exposures later in life remain unknown. Pregnant dams of Sprague Dawley rats were gavage with vehicle, 100 or 750 mg/kg bw/day of DEHP from Gestational Day 14 (GND14) until birth. After resting for twenty days, at postnatal day 21 (PND21) male offspring were gavage with vehicle, DEHP (100 or 750 mg/Kg/d) for 14 days (PND21-35). On PND35, offspring were sacrificed, serum testosterone (T) levels, body and organ (testis, liver and kidney) weight were examined. The data reveals that DEHP 750 mg/Kg/d but not 100mg/Kg/d treatment were sufficient to cause damage to testis when exposures occurs during either gestational or peripubertal (PND21-35) period. Interestingly, our data also indicated that subsequent exposures to DEHP (100 mg/Kg/day) in utero and at peri-pubertal stage were also effective to causing significant injury to testis. The serum T levels were reduced in all DEHP treatment groups. Next, we investigated the effects of peripubertal exposure to DEHP on the susceptibility to injury by subsequent exposure during adulthood. PND21 rats were subjected to oral administration of Oil, DEHP (100mg/kg/day or 750mg/kg/day) for 14 days (PND21-35). The rats were allowed to rest for 25 days until PND60. At PND60, rats were divided into groups and subsequently exposed to oil or DEHP (100 or 750 mg/Kg/d) for 14days (PND60-74). At PND74 the rats were sacrificed, serum T levels, body and organ weights were determined. Interestingly, no significant injury was observed in any of the treatment groups. Instead, and enhanced serum T levels were observed in DEHP-treated adult rats compared to control. Together, this study shows that the susceptibility to injuries following subsequent DEHP exposure is age-dependent, with immature male rats more susceptible while adults are resistant. In addition, the data also indicated that injuries sustained during peri-pubertal exposure are at least in part reversible. This work was supported by NIH-NIGMS-SCORE 2 grant SC2GM099578. Citation Format: Kassim Traore. Age-related difference in the susceptibility phthalate-induced injury in Sprague-Dawley rat. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1580. doi:10.1158/1538-7445.AM2014-1580
The impact of kidney function on outcomes following high risk myocardial infarction: findings from 27 610 patients
Authors: George V. Moukarbel, Zi‐Fan Yu, Kenneth Dickstein, Yingxin (Rachel) Hou, Janet T. Wittes, John J. V. McMurray, Bertram Pitt, Faiez Zannad, Marc A. Pfeffer, Scott D. Solomon
Journal: European Journal of Heart Failure (2013)
Government's plans for private practice are “insulting”
Authors: Linda Beecham
Journal: BMJ (2000)

Consultants' negotiators have criticised the government's proposals in The NHS Plan to make new hospital consultants in England work exclusively for the NHS (5 August, p 317).

The government suggested that consultants could work for the NHS for “perhaps the first seven years of their career” and has proposed restrictions on the conduct of private practice for other consultants.

At a special meeting this week the negotiators who work for the 25 000 consultants in the United Kingdom described the proposals for an exclusive NHS contract as “unnecessary, contradictory, …
Large Bowel Disorders
Authors: Giovanni A. Fava, Luigi Pavan
Journal: Psychotherapy and Psychosomatics (1976)

Stressful life events preceding disease onset were investigated in a consecutive series of 60 patients with large bowel disorders (ulcerative colitis, irritable bowel syndrome and appendicitis), using Paykel's methodology. Ulcerative colitis and irritable bowel syndrome were frequently preceded by events generally regarded as undesirable and involving losses or exits from the social field, which would be specific of a depressed population, while appendicitis seemed to reflect more generic psychosocial difficulties.
Aftermath of a disaster: the collapse of the Hyatt Regency Hotel skywalks
Authors: Charles B. Wilkinson
Journal: American Journal of Psychiatry (1983)

The author provides data regarding psychiatric symptoms reported by 102 persons who had experienced the collapse of two skywalks in the lobby of the Hyatt Regency Hotel in Kansas City, Mo. Those interviewed were injured victims, guests of the hotel who were not injured, and rescue workers. They were interviewed within 5 months of the disaster. Virtually all of the subjects had psychiatric symptoms; only slight differences were found among those who were victims, observers, or rescuers.
Oral Roentgenology. A Roentgen Study of the Anatomy and Pathology of the Oral Cavity.
Authors: Kurt Hermann Thoma
Journal: JAMA (1923)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal