tbuckley/cabot-search huggingface dataset:
Five-year follow-up in healthcare workers tested with tuberculin skin test and interferon gamma assay
Authors: Nittha Oer-Areemitr, Anan Wattanathum
Journal: European Respiratory Journal (2014)

Background : Thailand is a high burden country of tuberculosis and healthcare workers (HCWs) are significantly increased risk of mycobacterium tuberculosis (TB) infection. Treatment of Latent tuberculosis infection (LTBI) in Thailand is not routinely practice. Data about turning from LTBI to active TB among HCWs is limited.

Methods : We retrospectively reviewed the data from 284 HCWs in Phramongkutklao hospital who were tested for LTBI in 2007 and 2008. Both Interferon Gamma Release Assay (IGRA) and Tuberculin Skin Test (TST) were done. These HCWs were contacted in 2012 and 2013 for clinical evaluation and chest radiography to determine whether they had developed tuberculosis disease or not.

Results : Two hundred fifty six HCWs from 284 HCWs (90.1%) were able to contact. None of them received the treatment for LTBI. Eight HCWs were diagnosed TB disease (3.16%), 5 of them were pulmonary TB, 2 HCWs were TB pleuritis and 1 HCW was TB lymphadenitis).

Seventy-seven (30%) HCWs were diagnosed LTBI by IGRA positive (LTBI-IGRA group) and 179 (70%) HCWs were IGRA negative. The incidence of TB disease was 6 HCWs (7.8%) in LTBI-IGRA positive group and 2 HCWs (1.1%) in IGRA negative group (p=0.0049).

While comparing to TST method, 71 HCWs had TST positive (LTBI-TST group) and 185 HCWs had TST negative. Four HCWs in TST positive group had developed TB disease and 4 HCWs in TST negative group had developed TB disease (5.4 percent and 2.1 percent in LTBI-TST positive and negative, respectively), p=0.153.

Conclusions : To determine the LTBI among HCWs, IGRA positive group increased risk of TB disease significantly, whereas TST was unable to demonstrate the risk of TB disease.
Effects of Mitomycin C Alone and in Combination With X-Rays on EMT6 Mouse Mammary Tumors In Vivo&lt;xref ref-type="fn" rid="FN2"&gt;2&lt;/xref&gt;&lt;xref ref-type="fn" rid="FN3"&gt;3&lt;/xref&gt;
Authors: S Rockwell
Journal: JNCI Journal of the National Cancer Institute (1983)

The effects of mitomycin C alone and in combination with radiation on the cells of EMT6 mouse mammary tumors in BALB/cKaRw mice were examined. At doses near the toxic level, approximately 98% of the tumor cells were killed by a single injection of mitomycin C. Both proliferating and quiescent cells and both hypoxic and aerobic cells were killed by the drug. Cytotoxicity with mitomycin C occurred rapidly and was apparently complete within 30 minutes after injection of the drug . No evidence was found for repair of potentially lethal mitomycin C damage or sublethal mitomycin C damage by the tumor cells. Mitomycin C and radiation in combination produced an additive cytotoxicity; neither agent was found to alter significantly the shape of the dose-response curve for the other agent. The cytotoxicity of mitomycin C and radiation in combination depended on the sequence and timing of the treatments; additive toxicities were obtained when mitomycin C was given just after, just before, or up to 24 hours before irradiation, but the combination was less effective when mitomycin C was given 2-12 hours after irradiation.
Heterogeneity in the properties of mutant secreted lymphocyte antigen 6/urokinase receptor-related protein 1 (SLURP1) in Mal de Meleda
Authors: O. Adeyo, M. Oberer, M. Ploug, L.G. Fong, S.G. Young, A.P. Beigneux
Journal: British Journal of Dermatology (2015)

Journal Article Heterogeneity in the properties of mutant secreted lymphocyte antigen 6/urokinase receptor‐related protein 1 (SLURP1) in Mal de Meleda Get access O. Adeyo, O. Adeyo Department of Medicine David Geffen School of Medicine University of California Los Angeles 90095 CA U.S.A Search for other works by this author on: Oxford Academic Google Scholar M. Oberer, M. Oberer Institute of Molecular Biosciences University of Graz Humboldtstrasse 50/3 A‐8010 Graz Austria Search for other works by this author on: Oxford Academic Google Scholar M. Ploug, M. Ploug Finsen Laboratory Rigshospitalet Copenhagen Denmark Search for other works by this author on: Oxford Academic Google Scholar L.G. Fong, L.G. Fong Department of Medicine David Geffen School of Medicine University of California Los Angeles 90095 CA U.S.A Search for other works by this author on: Oxford Academic Google Scholar S.G. Young, S.G. Young Department of Medicine David Geffen School of Medicine University of California Los Angeles 90095 CA U.S.ADepartment of Human Genetics David Geffen School of Medicine University of California Los Angeles 90095 CA U.S.A Correspondence: Anne P. Beigneux and Stephen G. Young. E‐mails: abeigneux@mednet.ucla.edu; sgyoung@mednet.ucla.edu Search for other works by this author on: Oxford Academic Google Scholar A.P. Beigneux A.P. Beigneux Department of Medicine David Geffen School of Medicine University of California Los Angeles 90095 CA U.S.A Correspondence: Anne P. Beigneux and Stephen G. Young. E‐mails: abeigneux@mednet.ucla.edu; sgyoung@mednet.ucla.edu Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 173, Issue 4, 1 October 2015, Pages 1066–1069, https://doi.org/10.1111/bjd.13868 Published: 01 October 2015
Reaction of Immune Complexes With Hodgkin's Disease Tissue Cultures: Radioimmune Assay and Immunoferritin Electron Microscopy&lt;xref ref-type="fn" rid="FN2"&gt;2&lt;/xref&gt;
Authors: J C Long, A M Dvorak, S C Quay, C Stamatos, S Y Chi
Journal: JNCI Journal of the National Cancer Institute (1979)

We examined the binding of soluble immune complexes in sera from patients with Hodgkin's disease to established tissue cultures derived from the tumor. Circulating immune complex levels were determined by the Raji cell assay, and the reaction of serum with cultured cells was examined with a radioimmune assay and by immunoferritin electron microscopy. Serum with elevated immune complexes was found to react with cells of Hodgkin's disease monolayers when tested with radioiodine-labeled antisera against human IgG heavy and light chains and the complement 3 (C3) component. When examined with the electron microscope, monolayers incubated with Hodgkin's disease serum containing immune complex and labeled with ferritin-conjugated antiserum to C3 contained surface-bound ferritin particles with a uniform but discontinuous pattern. Absorption of Hodgkin's disease serum with monolayer cells reduced immune complexes and decreased reactivity of the sample with cultured cells by radioimmune assay. Sera of patients with other disorders and aggregated gamma-globulin with complement, despite markedly elevated immune complex levels, did not react positively with monolayers derived from Hodgkin's disease tumors, and none of the sera reacted with normal cultured spleen. The approximate size of serum components reacting with Hodgkin's disease monolayers was estimated by sucrose density gradient centrifugation. Sedimentation fractions in the 19S region reacted with monolayer cells when tested with 125I-labeled antisera to both IgG and C3 and contained immunoglobulin-complement complexes by gel diffusion and immunoabsorption. A component sedimenting at 7-9S contained immunoglobulin not complexed with complement; this component reacted with monolayer cells when tested with anti-IgG antiserum but did not react when tested with antibody to C3. The reaction of Hodgkin's disease monolayers with serum containing immune complexes differed from that of two suspension culture lines composed of cells with surface complement and IgG Fc receptors. Inasmuch as cells of our long-term Hodgkin's disease monolayers do not contain these surface receptors, possibly the antibody component of the immune complex reacts with antigens on the surface of cultured cells.
The Plasma Cell Signature in Autoimmune Disease
Authors: Katie Streicher, Christopher A. Morehouse, Christopher J. Groves, Bhargavi Rajan, Fernanda Pilataxi, Kim P. Lehmann, Philip Z. Brohawn, Brandon W. Higgs, Kathleen McKeever, Steven A. Greenberg, David Fiorentino, Laura K. Richman, Bahija Jallal, Ronald Herbst, Yihong Yao, Koustubh Ranade
Journal: Arthritis & Rheumatology (2013)

Production of pathogenic autoantibodies by self-reactive plasma cells (PCs) is a hallmark of autoimmune diseases. We undertook this study to investigate the prevalence of PCs and their relationship to known pathogenic pathways to increase our understanding of the role of PCs in disease progression and treatment response.We developed a sensitive gene expression-based method to overcome the challenges of measuring PCs using flow cytometry. Whole-genome microarray analysis of sorted cellular fractions identified a panel of genes, IGHA1, IGJ, IGKC, IGKV4-1, and TNFRSF17, expressed predominantly in PCs. The sensitivity of the PC signature score created from the combined expression levels of these genes was assessed through ex vivo experiments with sorted cells. This PC gene expression signature was used for monitoring changes in PC levels following anti-CD19 therapy, for evaluating the relationship between PCs and other autoimmune disease-related genes, and for estimating PC levels in affected blood and tissue from patients with multiple autoimmune diseases.The PC signature was highly sensitive and capable of detecting a change in as few as 360 PCs. The PC signature was reduced more than 90% in scleroderma patients following anti-CD19 treatment, and this reduction was highly correlated (r = 0.80) with inhibition of collagen gene expression. Evaluation of multiple autoimmune diseases revealed that 30-35% of lupus and rheumatoid arthritis patients had increased levels of PCs.This newly developed PC signature provides a robust and accurate method of measuring PC levels in the clinic. Our results highlight subsets of patients across multiple autoimmune diseases who may benefit from PC-depleting therapy.
In Vivo Inoculation of RNA C-Type Viruses Inducing Regression of Experimental Solid Tumors2
Authors: Joel S. Greenberger, Stuart A. Aaronson
Journal: JNCI Journal of the National Cancer Institute (1973)

Journal Article In Vivo Inoculation of RNA C-Type Viruses Inducing Regression of Experimental Solid Tumors Get access Joel S. Greenberger, Joel S. Greenberger Search for other works by this author on: Oxford Academic PubMed Google Scholar Stuart A. Aaronson Stuart A. Aaronson Viral Carcinogenesis Branch, National Cancer Institute, National Institutes of Health, Public Health Service, U.S. Department of Health, Education, and Welfare, Bethesda, Md. 20014 Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 51, Issue 6, December 1973, Pages 1935–1938, https://doi.org/10.1093/jnci/51.6.1935 Published: 01 December 1973 Article history Received: 04 June 1973 Accepted: 14 August 1973 Published: 01 December 1973
T cell development involves TRAF3IP3-mediated ERK signaling in the Golgi
Authors: Qiang Zou, Jin Jin, Yichuan Xiao, Hongbo Hu, Xiaofei Zhou, Zuliang Jie, Xiaoping Xie, James Y.H. Li, Xuhong Cheng, Shao-Cong Sun
Journal: The Journal of Experimental Medicine (2015)

Generation of T lymphocytes in the thymus is guided by signal transduction from the T cell receptor (TCR), but the underlying mechanism is incompletely understood. Here we have identified a Golgi-associated factor, TRAF3-interacting protein 3 (TRAF3IP3), as a crucial mediator of thymocyte development. TRAF3IP3 deficiency in mice attenuates the generation of mature thymocytes caused by impaired thymocyte-positive selection. TRAF3IP3 mediates TCR-stimulated activation of the mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase (ERK) and its upstream kinase mitogen/extracellular signal-regulated kinase (MEK). Interestingly, TRAF3IP3 exerts this signaling function through recruiting MEK to the Golgi and, thereby, facilitating the interaction of MEK with its activator BRAF. Transgenic expression of a constitutively active MEK rescues the T cell development block in Traf3ip3 knockout mice. These findings establish TRAF3IP3 as a novel regulator of T cell development and suggest a Golgi-specific ERK signaling mechanism that regulates thymocyte development.
Abstract P291: Psychosocial Factors are Associated with Cardiovascular Disease among African Americans in the Jackson Heart Study
Authors: Mario Sims, Marino Bruce, Sharon Wyatt, Tom Mosley, Daniel Sarpong, David Mount, Herman Taylor
Journal: Circulation (2014)

Introduction: African Americans (AA) have a higher risk for cardiovascular disease (CVD) than Whites. This disparity has been attributed to risk factors such as hypertension, diabetes, and obesity. This disparity has also been attributed to psychosocial factors across groups. Using Jackson Heart Study (JHS) data, we examined the associations of negative affect and stress measures with CVD risk factors and prevalent CVD among AA. Hypothesis: Negative affect and stress measures are associated with prevalent CVD risk factors and CVD among men and women in the JHS. Methods: Cross-sectional associations of negative affect (cynicism, anger in, anger out, and depressive symptoms) and stress (global stress, weekly stress, and major life events) with prevalent BMI, hypertension, diabetes and CVD were examined among 5,301 participants 34-85 years old (women=3,360; men=1,941). We estimated mean differences in BMI and prevalence ratios (PR) of hypertension, diabetes and CVD with measures of negative affect and stress and adjusted for demographic and clinical risk factors. Results: Men had higher cynical distrust and anger in scores than women (p&lt;.05). Women had higher depressive symptoms, global stress, weekly stress and major life event scores than men (p&lt;.05). After adjustment for age, sex, education, behaviors and risk factors, each psychosocial factor (except anger out and weekly stress-event) was associated with an increased mean average of BMI. For example, the mean difference in BMI increased by 41% for each 1 SD increase in cynical distrust (p&lt;.01); and the mean difference in BMI increased by 45% for each 1 SD increase in global stress (p&lt;.01). In fully-adjusted models, global perceived stress was associated with prevalent hypertension and diabetes (PR for hypertension: 1.07, 95%CI 1.00,1.07; PR for diabetes: 1.24, 95%CI 1.04, 1.47). Major life events were also associated with hypertension and diabetes. Each psychosocial measure (except cynicism and anger in) was associated with prevalent CVD. Conclusion: Efforts to reduce disparities in CVD may need to address environmental and psychosocial factors that place AA at higher risk.
Volatile organic compounds in lung cancer patients before and after tumour resection
Authors: Kaid Darwiche, Isabella Kurth, Joerg Ingo Baumbach, Urte Sommerwerck, Helmut Teschler, Lutz Freitag
Journal: European Respiratory Journal (2011)

Introduction: Ion mobility spectrometry (IMS) is a promising tool in the detection of volatile organic compounds (VOC) even in small amounts. Whether it can contribute to the diagnosis of non-small-cell cancers (NSCLC) has not been adequately evaluated. Breath analysis with IMS is based on the assessment of multiple volatile organic compound (VOC) peaks considered specific for the disease.

Objectives: We studied bronchoscopically obtained VOCs in exhaled breath with an ion mobility spectrometer coupled to a multi capillary column (MCC/IMS) in patients with NSCLC before and after tumour resection in order to find tumour specific VOCs.

Methods: In 8 patients with histologically proven NSCLC, gas samples were aspirated out of the lungs during the diagnostic bronchoscopy and after tumour resection. Gas samples were aspirated via a Teflon tube introduced in the working channel of the bronchoscope and assessed using IMS.

Results: We found 228 common peaks in the measured data. 17 of them were significantly different before and after surgery. 11 peaks could be found with a lower value after tumour resection whereas 6 had a higher value.

Conclusion: While in a former study (Poli D et al. Acta Biomed 2008; 79; Suppl 1: 64-72) using solid-phase micro-extraction no changes after lung cancer resection could have been found, IMS revealed a change in the composition of exhaled breath after surgery in our work. Therefore some VOC levels may have been influenced by the tumour and these VOCs can be detected by MCC/IMS.
Abstract 3461: Contrast-Induced Nephropathy After Percutaneous Coronary Intervention is Associated With High Long-Term Mortality: Insights From CREDO-Kyoto Registry
Authors: Mitsuru Abe, Ryoji Taniguchi, Natsuhiko Ehara, Naritatsu Saito, Satoshi Shizuta, Yutaka Furukawa, Katsuo Okazaki, Takahiro Sakurai, Kazuaki Kataoka, Hisao Ogawa, Michio Kawasuji, Taku Hirai, Sachiko Maeda, Takeshi Morimoto, Satoshi Teramukai, Masanori Fukushima, Toru Kita, Takeshi Kimura
Journal: Circulation (2007)
Tumor-Specific Antigen(s) in a Spontaneous Mammary Carcinoma of C3H Mice. II. Active Immunization of Mammary-Tumor-Agent-Free Mice&lt;xref ref-type="fn" rid="FN1"&gt;2&lt;/xref&gt;
Authors: H. D. Suit, V. Silobrcic
Journal: JNCI Journal of the National Cancer Institute (1967)

Journal Article Tumor-Specific Antigen(s) in a Spontaneous Mammary Carcinoma of C3H Mice. II. Active Immunization of Mammary-Tumor-Agent-Free Mice Get access H. D. Suit, H. D. Suit Section of Experimental Radiotherapy, The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77025 Search for other works by this author on: Oxford Academic PubMed Google Scholar V. Silobrcic V. Silobrcic Section of Experimental Radiotherapy, The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77025 Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 39, Issue 6, December 1967, Pages 1121–1128, https://doi.org/10.1093/jnci/39.6.1121 Published: 01 December 1967 Article history Received: 09 March 1967 Revision received: 30 June 1967 Published: 01 December 1967
Resistance to Arteriosclerosis in Pigs with von Willebrand's Disease
Authors: Valentin Fuster, E. J. Walter Bowie, Jon C. Lewis, David N. Fass, Charles A. Owen, Arnold L. Brown
Journal: Journal of Clinical Investigation (1978)

The aortas of 11 pigs (aged 1-3 yr) with homozygous von Willebrand's disease (vWd) were compared with those of 11 normal pigs of the same ages. Six of the controls exhibited multiple arteriosclerotic plaques with intimal thickening of 63-130 μm. In contrast, none of the pigs with vWd had multiple plaques, and only one had a lesion >2 mm in diameter.
Active Specific Immunotherapy of Established Micrometastasis: Effect of Cryopreservation Procedures on Tumor Cell Immunogenicity in Guinea Pigs23
Authors: Leony C. Peters, Michael G. Hanna
Journal: JNCI Journal of the National Cancer Institute (1980)

In a transplantable hepatocarcinoma (L10) model of inbred Sewall Wright strain 2 guinea pigs, estabilshed micrometastatic tumor foci could be eliminated from the viscera by specific tumor immunity induced by the systemic effect of a BCG-L10 tumor cell vaccine. The conditions of vaccine preparation and regimen were rigid, and a dose dependency for both BCG and tumor cells existed. Comparison of the influence of cryobiologic preservation procedures on the tumor cells to be used in the vaccine showed that cells frozen by an optimized cryobiologic procedure, improved for maintenance of viable cells, were more effective than cells frozen by a conventional glycerol method. The glycerol method resulted in a lower percentage of viable cells, and the effectiveness of the vaccine was greatly diminished. Thus some past failures of active specific immunotherapy could be associated with acute antigenic exposures resulting from suboptimal cryobiologic preservation of tumor cells in the vaccine preparation instead of prolonged or chronic tumor cell antigenic exposure resulting from optimal cryobiologic procedures.
Aclidinium bromide reduces epithelial-mesenchymal transition of human COPD epithelial cells
Authors: Christopher Lambers, Katrin Hostettler, Nicola Miglino, Elisabeth Schuller, Michael Roth, Lutz-Henning Block
Journal: European Respiratory Journal (2013)

Background:  Epithelial-mesenchymal transition (EMT) is assumed to contribute to COPD pathogenesis leading to changes in tissue structure.   Objective:  The effect of aclidinium and formoterol, alone and in combination, on TGF-β1 and cigarette smoke (CS)-induced EMT in primary human epithelial lung cells from patients with stage II COPD (n=6) and non-COPD controls (n=6).   Methods:  EMT was determined by light microscopy phenotyping of primary cells from lung tissue biopsies. Epithelial cells were defined by knobble-stone shaped and positive staining for E-cadherin; fibroblasts by spindle-shaped cells staining positive for fibronectin. Cells were stimulated over 3 days with either TGF-β1 (5 pg/mL) or with CS-conditioned medium. 1    Results:  TGF-β1 and CS-conditioned medium induced rapid loss of epithelial cell characteristics both in shape and for E-cadherin expression. Within 48 hours >85% of epithelial cells transitioned into fibroblast-like and fibronectin positive cells. Pre-incubation (30 min) with either aclidinium or formoterol (both: 10 -11 -10 -8  M) alone reduced EMT in COPD epithelial cells by 10% and 22%, respectively, but not in cells obtained from non-COPD (control) patients. The drug9s combined effect was dose dependent and significantly synergistic on TGF-β1 and CS-induced EMT than the drugs alone.   Conclusion : The combination of aclidinium and formoterol has a greater effect on reducing EMT in human epithelial cells compared with either drug alone.   Clinical implication:  Adding aclidinium, formoterol, or a combination of aclidinium and formoterol to COPD therapy may reduce EMT and thereby slow down the progression of COPD.   1 Miglino et al. Eur Respir J 2012;39:705-711.
Fibroblasts Rendered Antifibrotic, Antiapoptotic, and Angiogenic by Priming With Cardiosphere-Derived Extracellular Membrane Vesicles
Authors: Eleni Tseliou, Joseph Fouad, Heidi Reich, Leandro Slipczuk, Geoffrey de Couto, Mark Aminzadeh, Ryan Middleton, Jackelyn Valle, Liu Weixin, Eduardo Marbán
Journal: Journal of the American College of Cardiology (2015)
US navy will not discharge nurse who refused to force feed Guantanamo prisoners
Authors: M. McCarthy
Journal: BMJ (2015)

The US navy will not discharge the naval medical officer, a registered nurse, who refused to participate in the force feeding of prisoners engaged in hunger strikes at the US naval station in Guantanamo Bay, Cuba, the officer’s lawyer has said.

“Now he can go back to practicing nursing, finish out his time in the navy, and retire honorably,” said Ron Meister, the New York attorney representing the officer. The officer’s name has not been disclosed.

The case was brought to light last year after …
Role of Bone Marrow Evaluation (Bme) in Primary Central Nervous System Lymphoma (Pcnsl) in a Multiracial Asian Population
Authors: Y.J. Wong, T. Jing Ying, None Tira, K. Lay Poh, Y. Sook Kwin, M. Tao, R.Q. Hong Hui, T.P.L. Tang, M.F. Harunal Rashid, Y.T. Goh, C.P. Diong, L.H.C. Tan, S.Y. Tan, S.T. Lim
Journal: Annals of Oncology (2014)