tbuckley/cabot-search huggingface dataset:
Clinical diagnosis and risk stratification in patients with brugada syndrome∗∗Editorials published in the Journal of the American College of Cardiologyreflect the views of the authors and do not necessarily represent the views of JACCor the American College of Cardiology.
Authors: Ihor Gussak, Preben Bjerregaard, Stephen C Hammill
Journal: Journal of the American College of Cardiology (2001)
Abstract 844: Contribution of dual oxidase 2 (DUOX2) to hyper radiosensitivity in human gastric cancer cells
Authors: Duc Nguyen, Elizabeth Chang, Navesh Sharma, France Carrier
Journal: Cancer Research (2014)

Abstract Whole abdominal radiotherapy (WART) is a primary method for managing gastrointestinal cancers that have disseminated into intra-abdominal tissues. While effective, this approach is limited since combination of WART with full-dose chemotherapy regimens increase toxicity to normal tissue. Recent studies have demonstrated a survival advantage in a novel treatment paradigm that allows for the safe use of full-dose systemic chemotherapy in combination with Low Dose Fractionated Radiotherapy (LDFRT). Traditionally, radiotherapy used doses greater than 120 cGy because it was thought that lower radiation doses would be ineffective for tumor therapy. We now know that LDFRT can produce hyper-radiosensitivity (HRS), a phenomenon where cells undergo apoptosis at radiation doses as low as 15 cGy, in a number of proliferating cells. Here we aimed at determining whether LDFRT can induce HRS in gastrointestinal cancer cells and at identifying biomarkers of chemopotentiation by LDFRT. Our data indicate that three consecutive daily fractions of 15 cGy produced HRS in gastric cancer cells and potentiated a modified regimen of Docetaxel, Cisplatin, and 5’-fluorouracil (mDCF). Colony survival assays indicated that 15 cGy was sufficient to kill 90% of the cells when LDFRT was combined with mDCF whereas an almost ten times higher dose (135 cGy) was needed to achieve the same rate when using conventional radiotherapy alone. RT2 PCR Profiler Array analysis indicated that this combined regimen up-regulated dual oxidase 2 (DUOX2), an enzyme functioning in the production of hydrogen peroxide, without up-regulating genes involved in DNA repair. Moreover, down regulation of DUOX2 increased radiation resistance at every radiation doses tested. In addition, a subset of genes known to be activated by Reactive Oxygen Species were specifically upregulated by LDFRT when combined with mDCF. Taken together these data suggest that chemopotentiation by LDFRT in gastric cells may be due, at least in part, to increased ROS production (DUOX2) without upregulation of the DNA repair machinery. These data thus provide a rationale for further explorations of potential clinical applications of LDFRT, such as in WART, as chemopotentiator for advanced and metastatic gastric cancers. Citation Format: Duc Nguyen, Elizabeth Chang, Navesh Sharma, France Carrier. Contribution of dual oxidase 2 (DUOX2) to hyper radiosensitivity in human gastric cancer cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 844. doi:10.1158/1538-7445.AM2014-844
Computerized quantification of wheezing in neonates: Relationship with conventional lung function parameters
Authors: Hendrik Fischer, Silke Wilitzki, Hans Proquitté, Charles Roehr, Gerd Schmalisch
Journal: European Respiratory Journal (2012)

Objective:  Computerized respiratory sound analysis has been used to evaluate wheeze in infants, but it is not known whether the acoustic detection of wheeze is associated with impaired lung function. The present study aimed to investigate the relationship between wheeze detection and conventional parameters of lung function testing (LFT).   Methods:  Computerized lung sound analysis with quantification of wheezing (PulmoTrack®) was performed in 78 neonates at a median (IQR) postmenstrual age of 48 (44-59) weeks and a body weight of 4818 (3835–6140) g. In the same session, LFT was performed which included bodyplethysmography, tidal breathing measurements, SF6 multiple breath washout, measurement of respiratory mechanics by the occlusion test, forced expiratory flow (V9maxFRC) by rapid thoracoabdominal compression and capillary blood gas analysis.   Results:  Wheezing >5% of the breathing cycle was detected in 41/78 (53%) infants, 18/78 (23%) had inspiratory and 29/78 (37%) expiratory wheezing. Infants with and without wheezing did not significantly differ in postmenstrual age and body weight. Inspiratory wheezing had no relationship with LFT parameters. In contrast, infants with expiratory wheezing had significant changes in their breathing pattern and respiratory mechanics with increased tPTEF/TE (p=0.034), end-expiratory flow (TEF10, p=0.027), airway resistance (p=0.005) and respiratory resistance (p=0.036).   Conclusion:  Computerized wheeze detection is easy to perform in unsedated infants during natural sleep and may help to identify infants at risk of obstructive airway disease.
Abstract 15738: Electrical Stimulation Enhances Efficiency of Cardiac Differentiation of Human Induced Pluripotent Stem Cells by Regulating P38 Map Kinase
Authors: Ruilian Ma, Linlin Guo, Lei Wang, Hideyuki Kondo, Ronald Millard, Ha Won Kim, Yi-Gang Wang
Journal: Circulation (2014)

Background: Induced pluripotent stem cells (iPSC) can efficiently differentiate into functional cardiomyocytes through embryoid body (EB) differentiation in the presence of specific differentiation signals (e.g. TGF-β, Wnts). Recent studies have indicated that electrical stimulation (EleS) can enhance proliferation and differentiation efficacy in various stem cells. This study investigated the effects of EleS on human iPSC differentiation into cardiomyocytes and explored the underlying mechanisms. Methods and Results: The classical hanging-drop method was used to induce EB formation from human iPSCs. EBs were then treated with or without EleS (1-1.5 V/1.8 cm with 5ms biphasic square wave pulses at 5 Hz) for 1 to 30 days. The percentage of contracting EBs as well as contraction rate was examined. EBs started to beat at 2 days after EleS while spontaneous beating cells without EleS appeared at 7 days. In addition, the percentage of contracting cardiac progenitor cells (CPCs) and contraction rate were signif...
How the New Neuroscience Will Advance Medicine
Authors: Cornelia I. Bargmann
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
The synergic effect of vincristine and vorinostat in leukemia in vitro and in vivo
Authors: Min-Wu Chao, Mei-Jung Lai, Jing-Ping Liou, Ya-Ling Chang, Jing-Chi Wang, Shiow-Lin Pan, Che-Ming Teng
Journal: Journal of Hematology & Oncology (2015)

Combination therapy is a key strategy for minimizing drug resistance, a common problem in cancer therapy. The microtubule-depolymerizing agent vincristine is widely used in the treatment of acute leukemia. In order to decrease toxicity and chemoresistance of vincristine, this study will investigate the effects of combination vincristine and vorinostat (suberoylanilide hydroxamic acid (SAHA)), a pan-histone deacetylase inhibitor, on human acute T cell lymphoblastic leukemia cells.Cell viability experiments were determined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, and cell cycle distributions as well as mitochondria membrane potential were analyzed by flow cytometry. In vitro tubulin polymerization assay was used to test tubulin assembly, and immunofluorescence analysis was performed to detect microtubule distribution and morphology. In vivo effect of the combination was evaluated by a MOLT-4 xenograft model. Statistical analysis was assessed by Bonferroni's t test.Cell viability showed that the combination of vincristine and SAHA exhibited greater cytotoxicity with an IC50 value of 0.88 nM, compared to each drug alone, 3.3 and 840 nM. This combination synergically induced G2/M arrest, followed by an increase in cell number at the sub-G1 phase and caspase activation. Moreover, the results of vincristine combined with an HDAC6 inhibitor (tubastatin A), which acetylated α-tubulin, were consistent with the effects of vincristine/SAHA co-treatment, thus suggesting that SAHA may alter microtubule dynamics through HDAC6 inhibition.These findings indicate that the combination of vincristine and SAHA on T cell leukemic cells resulted in a change in microtubule dynamics contributing to M phase arrest followed by induction of the apoptotic pathway. These data suggest that the combination effect of vincristine/SAHA could have an important preclinical basis for future clinical trial testing.
THE TREATMENT OF RHEUMATIC FEVER BY ROENTGEN-RAY IRRADIATION
Authors: Geo. C. Griffith, E. P. Halley
Journal: Annals of Internal Medicine (1946)

Article1 June 1946THE TREATMENT OF RHEUMATIC FEVER BY ROENTGEN-RAY IRRADIATIONGEO. C. GRIFFITH, E. P. HALLEYGEO. C. GRIFFITHSearch for more papers by this author, E. P. HALLEYSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-24-6-1039 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptINTRODUCTIONIt is an accepted medical fact that in the absence of a specific therapeutic agent for a given disease entity there are many non-specific methods of treatment proposed. Because of the prevalence of rheumatic fever and the urgency in wartime to marshall only the best of the available therapeutic armamentaria, an evaluation of roentgen-ray irradiation in rheumatic fever is apropos.From 1926 to 1933 the most significant studies in this field were published by Levy and Golden.1A careful review of the literature concerning the effects of roentgen irradiation on the heart was published by the same authors.1bIn...Bibliography1. LEVYGOLDEN RLR: Roentgen-ray therapy in rheumatic heart disease, Proc. Soc. Exper. Biol. and Med., 1926, xxiii, 351-353. CrossrefGoogle Scholar2. LEVYGOLDEN RLR: Effects of irradiation of heart in rheumatic carditis, Am. Jr. Roent. and Rad. Therapy, 1927, xviii, 103-110. Google Scholar3. LEVYGOLDEN RLR: Treatment of rheumatic carditis by roentgen irradiation of heart, Am. Heart Jr., 1928, iv, 127-144. CrossrefGoogle Scholar4. LEVYGOLDEN RLR: Roentgen therapy of rheumatic heart disease; review of 6 years' experience, Am. Jr. Roent. and Rad. Therapy, 1933, xxix, 79-82. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: *Received for publication September 29, 1945. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byRHEUMATISM AND ARTHRITIS REVIEW OF AMERICAN AND ENGLISH LITERATURE OF RECENT YEARS (TENTH RHEUMATISM REVIEW)* Part IIWILLIAM D. ROBINSON, M.D., F.A.C.P., EDWARD W. BOLAND, M.D., F.A.C.P., JOSEPH J. BUNIM, M.D., F.A.C.P., DARRELL C. CRAIN, M.D., F.A.C.P., EPHRAIM P. ENGLEMAN, M.D., F.A.C.P., WALLACE GRAHAM, M.D., F.R.C.P. (C), L. MAXWELL LOCKIE, M.D., F.A.C.P., MAX M. MONTGOMERY, M.D., F.A.C.P., CHARLES RAGAN, M.D., F.A.C.P., MARIAN W. ROPES, M.D., F.A.C.P., EDWARD F. ROSENBERG, M.D., F.A.C.P., CHARLEY J. SMYTH, M.D. 1 June 1946Volume 24, Issue 6Page: 1039-1042KeywordsHeartHyperthermiaLongitudinal studiesRheumatic fever ePublished: 1 December 2008 Issue Published: 1 June 1946 PDF downloadLoading ...
Relationship of Serum Inflammatory Biomarkers With Plaque Inflammation Assessed by FDG PET/CT
Authors: Raphaël Duivenvoorden, Venkatesh Mani, Mark Woodward, David Kallend, Gabriela Suchankova, Valentin Fuster, James H.F. Rudd, Ahmed Tawakol, Michael E. Farkouh, Zahi A. Fayad
Journal: JACC. Cardiovascular imaging (2013)
Health information on internet is often unreliable
Authors: Scott Gottlieb
Journal: BMJ (2000)

Open access to biomedical information on the internet and through other easily accessible electronic databases has created new opportunities for doctors and patients, but much of the information is subject to manipulation because the ordinary conventions of context and the reliability of provenance are constantly in question.

That was one of the major themes of “Freedom of Information,” a conference held in New York's Academy of Medicine on 6 and 7 July, sponsored by BioMed Central, which publishes peer reviewed clinical research reports that are available through the internet.

“On the internet anything goes and that's all right,” said George Lundberg, editor of the online medical website Medscape.com and former editor in chief of the journal of the American …
Surface topography enhances differentiation of mesenchymal stem cells towards osteogenic and adipogenic lineages
Authors: Giulio Abagnale, Michael Steger, Vu Hoa Nguyen, Nils Hersch, Antonio Sechi, Sylvia Joussen, Bernd Denecke, Rudolf Merkel, Bernd Hoffmann, Alice Dreser, Uwe Schnakenberg, Arnold Gillner, Wolfgang Wagner
Journal: Biomaterials (2015)
Abstract P261: Methylation Status of VKORC1 and Warfarin Dose
Authors: Brian S Finkelman, Luanne Bershaw, Jinbo Chen, Colleen M Brensinger, Ron C Li, Stephen E Kimmel
Journal: Circulation (2014)

Introduction: It is well known that genetic variants, especially in the VKORC1 and CYP2C9 genes, can be associated with warfarin maintenance dose. However, currently known genetic variants fail to explain a large portion of the observed variability in warfarin dose, especially in African American patients. Methylation is a heritable epigenetic modification in which conversion of cytosine residues within “CpG islands” to 5-methylcytosine is associated with reduced gene expression. To our knowledge, the effect of methylation status on warfarin dose has never been studied. Hypothesis: We hypothesized that higher rates of methylation near the start region of VKORC1 in whole blood would be associated with lower required maintenance dose in warfarin patients. Methods: We conducted a prospective cohort study of patients initiating warfarin at three anticoagulation clinics. All individuals with available methylation data who reached maintenance dose were included in the analysis (N = 237). Methylation status was assessed in the region from 1500bp upstream to 500bp downstream of the VKORC1 start site using the Sequenom EpiTYPER assay on DNA extracted from whole blood. Linear regression was used to assess the association between methylation levels at individual CpG sites (66 total) and required warfarin maintenance dose. Visual inspection of univariable results was used to identify potentially important CpG clusters, which were subsequently examined using univariable and multivariable linear regression, with the latter adjusting for the effects of race, presence of VKORC1 variant, and other well-known predictors of warfarin dose. Results: Two of 66 CpG sites were nominally associated with lower maintenance dose requirement on univariable analysis. These CpG sites were identified as part of a CpG cluster located approximately 330 to 226bp upstream of the VKORC1 start site. Median methylation levels in this cluster was 16% (IQR 15%, 17%). A 1% increase in methylation in this cluster was associated with a 2.8 mg/wk reduction in weekly warfarin maintenance dose (P = 0.02). No significant interaction between methylation status and African American race was observed (P = 0.77). This association was substantially attenuated with the inclusion of VKORC1 variant status in the model (P = 0.33). Conclusions: Preliminary evidence suggests that methylation levels in a CpG cluster in VKORC1 may be moderately associated with warfarin maintenance dose, although this association may not be independent of VKORC1 variant status. However, these results require confirmation from additional epidemiological studies and possibly functional assays in liver tissue.
Abstract 20426: Study of S-Nitrosylation in Duchenne Muscular Dystrophy
Authors: Heaseung S Chung, Peter P Rainer, David A Kass, Jennifer E Van Eyk
Journal: Circulation (2014)

Introduction: Severe cardiac myopathy occurs with Duchenne muscular dystrophy (DMD), a fatal disease characterized by absence of functional dystrophin protein. The mechanisms of the disease in cardiac muscle and whether it mimics some of the pathophysiological mechanisms present in skeletal muscle are not clear. The loss of dystrophin was reported to disrupt neuronal nitric oxide synthase mu, presumably altering redox-signaling. Nitric oxide-induced S-nitrosylation (SNO) on cysteine residues is known as a redox-sensor, which responds to cellular dynamic redox-environment. Here we identified and quantified the SNO-modified sites of cardiac tissue in dystrophy model using a novel proteomics approach. Methods: SNO-proteins from cardiac tissues of 10 months-old mdx/utrn+/- (n=3) and C57BL/6 (n=3) mice were detected with our improved TMT (tandem mass tag)-switch technique coupled with liquid chromatography/tandem mass spectrometry. The identified SNO of each site was quantified across DMD and WT samples and the fold change of SNO on each site in DMD compared to WT was displayed as ‘SNO-index (I)’, calculated by SNO DMD /SNO WT . Results: Proteomics analysis identified total 866 SNO-modified peptides on 287 SNO-proteins in the dystrophy cardiac tissue. The magnitudes of SNO-fold change were various across peptides (I=0.1 least to 1702.7 most). 32% of these, 278 peptides were more than two-fold oxidized in DMD than WT. Interestingly, a termed ‘Dilated cardiomyopathy’ protein group by KEGG-pathway analysis has extremely oxidized sites than other groups, which are more than 10-folds S-nitrosylated in DMD. Myosin-6 at Cys949 and Cys1750 (I=11.4, 117.3), Cys432 of myosin-binding protein C (I=135.0) and Cys191 of myosin light chain 3 (I=81.2) displayed a considerable SNO-increase in dystrophy sample and Cys35 of troponin C was also more modified (I=2.9), while other sites such as Cys397 of creatine kinase S-type was less oxidized (I=0.26) in DMD. Conclusions: Our novel TMT-switch assay allowed quantification of the very labile SNO-modification as well as site-identification. More than 500 cysteine residues sensed the oxidative stimuli and each had a specific susceptibility to SNO. The SNO-based redox switches were shifted in DMD compared with WT.
Of the Profession, by the Profession, and for Patients, Families, and Communities
Authors: Lois Margaret Nora, Matthew K. Wynia, Thomas Granatir
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Neutrophil gelatinase B potentiates interleukin-8 tenfold by aminoterminal processing, whereas it degrades CTAP-III, PF-4, and GRO-α and leaves RANTES and MCP-2 intact
Authors: Philippe E. Van den Steen, Paul Proost, Anja Wuyts, Jo Van Damme, Ghislain Opdenakker
Journal: Blood (2000)
Abstract 19725: Prevalence and Clinical Phenotype of Sodium and Calcium Channel Mutations in Brugada Syndrome
Authors: Christian Veltmann, Andrea Krey, Christian Wolpert, Boris Rudic, Torsten Konrad, Florian Streitner, Nina Schoene, Juergen Kuschyk, Ryan Pfeiffer, Elena Burashnikov, Gabriel Caceres, Martin Borggrefe, Rainer Schimpf, Charles Antzelevitch
Journal: Circulation (2010)
Prophylactic cranial irradiation in small cell lung cancer: a systematic review of the literature with meta-analysis.
Authors: Anne-Pascale Meert, Marianne Paesmans, Thierry Berghmans, Benoît Martin, Céline Mascaux, Frédéric Vallot, Jean-Marc Verdebout, Jean-Jacques Lafitte, Jean-Paul Sculier
Journal: European Respiratory Journal (2001)

A systematic review of the literature was carried out to determine the role of prophylactic cranial irradiation (PCI) in small cell lung cancer (SCLC) . To be eligible, full published trials needed to deal with SCLC and to have randomly assigned patients to receive PCI or not. Trials quality was assessed by two scores (Chalmers and ELCWP). Twelve randomised trials (1547 patients) were found to be eligible. Five evaluated the role of PCI in SCLC patients who had complete response (CR) after chemotherapy. Brain CT scan was done in the work-up in five studies and brain scintigraphy in six. Chalmers and ELCWP scores are well correlated (p < 0.001), with respective median scores of 32.6 and 38.8 %. This meta-analysis based on the available published data reveals a decrease of brain metastases incidence (hazard ratio (HR): 0.48; 95 % confidence interval (CI): 0.39 - 0.60) for all the studies and an improvement of survival (HR: 0.82; 95 % CI: 0.71 - 0.96) in patients in CR in favour of the PCI arm. Unfortunately, long-term neurotoxicity was not adequately described . PCI decreases brain metastases incidence and improves survival in CR SCLC patients but these effects were obtained in patients who had no systematic neuropsychological and brain imagery assessments. The long-term toxicity has not been prospectively evaluated. If PCI can be recommended in patients with SCLC and CR documented by a work-up including brain CT scan, data are lacking to generalise its use to any CR situations.
Case report of barbiturate-induced enhancement of methadone metabolism and withdrawal syndrome
Authors: S J Liu, R I Wang
Journal: American Journal of Psychiatry (1984)

A methadone maintenance patient with a decreased plasma methadone level, possibly due to phenobarbital-induced acceleration of methadone biotransformation, experienced opioid withdrawal symptoms. This finding suggests that when phenobarbital is used with methadone the plasma methadone level must be monitored.