tbuckley/cabot-search huggingface dataset:
Boats in Trouville Harbor
Authors: M Therese Southgate
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 13358: Monocyte and Macrophage-Directed Peptide Amphiphile Micelles Modulate Cytoskeletal Organization and Target Atherosclerosis
Authors: Danielle Hyatt, Eun Ji Chung, Matthew Tirrell, Francis J Alenghat
Journal: Circulation (2014)

Background: Atherosclerosis is a multifactorial disease with a well-established inflammatory component. Diagnostic imaging technologies have made great strides to define the degree of atherosclerotic burden. However, there may be additional benefit to detecting vulnerable, more inflammatory plaques. Such an approach could have diagnostic and prognostic value, and by targeting pathological cells of an atheroma, may also provide opportunities to deliver disease-modifying therapy. Results: To this end, we characterized monocyte-targeting, peptide amphiphile micelles (PAMs) that use the chemokine receptor CCR2-binding motif of monocyte chemoattractant protein-1 (MCP-1). These MCP-1 PAMs bind to monocytes and macrophages in vitro and exhibit some biofunctional properties of the MCP-1 native protein. Compared to PAMs containing scrambled peptides, MCP-1 PAMs induced increased F-actin polymerization in a murine macrophage cell line derived from WEHI-274.1 cells (0.99 ± 0.02 vs. 1.24 ± 0.03 units, n=50, p < 5 x 1...
Abstract 17035: Identification of Novel Circulating Multipotent Stem Cells in Human Peripheral Blood, Which Originate From Human Heart
Authors: Han-Mo Yang, Ju-Young Kim, Joo-Eun Lee, Hyun-Jai Cho, Hae-Young Lee, Hyun-Jae Kang, Byung-Hee Oh, Young-Bae Park, Hyo-Soo Kim
Journal: Circulation (2011)

Background: There has been only one study showing the presence of multipotent stem cells in bone marrow, called multipotent adult progenitor cells (MAPCs). These cells can be differentiated not only into mesenchymal lineage cells, but also into cells with neuroectoderm or endoderm features. Until recently, however, there has been no report isolating and characterizing these cells from human peripheral blood. Here, we hypothesized that there might be circulating multipotent stem cells in human peripheral blood. In addition, we tried to find the origin of these cells. Methods and Results: From human peripheral blood mononuclear cells adherent on non-coated dishes, we could identify new population of cells, which are quite distinct from previously reported other stem cells. Newly identified cells have the gene profiles such as Oct3/4, KLF4, and c-Myc, suggesting multipotent stemness. Moreover, FACS analysis demonstrated that these stem cells have the property of mesenchymal stem cells. With diverse differentiation media, we could differentiate these cells into endothelial-like cells or vascular smooth muscle-like cells. These stem cells have the properties of osteogenic, adipogenic, and myogenic differentiation. Moreover, these cells can be differentiated into ectodermal lineage cells such as neuron-like cells as well as endodermal lineage cells such as hepatocyte-like cells. When injected into the mouse heart in vivo , these newly identified stem cells could be differentiated into endothelial cells, smooth muscle cells, and cardiomyocytes. To investigate the origin of these cells, we collected peripheral mononuclear cells from patients receiving the transplantation of bone marrow, liver, heart, or kidney. After culturing these cells and identifying HLA type of each cells, we could confirm that these stem cells are derived from human heart. Conclusion: This study showed the presence of novel circulating multipotent stem cells in human peripheral blood. Interestingly, these cells are derived from human heart. These findings suggest that these cells can be easily obtained from human peripheral blood and used for regenerative medicine in real world.
Sialic acid content of erythrocytes in normal individuals and patients with certain hematologic disorders
Authors: D. Aminoff, J. Anderson, L. Dabich, W.D. Gathmann
Journal: American Journal of Hematology (1980)

Abstract The sialic acid content of erythrocytes from healthy individuals of different blood types and of patients with known hematological disorders has been determined. The sialic acid was completely released enzymatically with sialidase and quantitated by the thiobarbituric acid method. The sialic acid content of erythrocytes was constant irrespective of ABO blood type, or anticoagulant used; viz, 0.85–0.92 m̈moles/ml of packed erythrocytes or 46–53 × 10 6 sialyl residues per cell. Deviations from these normal values were obtained with erythrocytes from patients with a variety of hematological disorders. Patients with the following disorders have significantly (P &lt; 0.01) lower sialic acid values compared to erythrocytes from healthy individuals (given in the order of decreasing sialic acid content): sickle cell anemia, acute lymphocytic leukemia, chronic lymphocytic leukemia, acute myelomonocytic leukemia, non‐Hodgkin lymphocytic lymphoma, chronic granulocytic leukemia, acute myelocytic leukemia, leukemia, and Hodgkin disease.
First outbreak of methicillin-resistant Staphylococcus aureus ST398 in a Dutch hospital, June 2007
Authors: M WH Wulf, A Markestein, F T van der Linden, A Voss, C Klaassen, C M Verduin
Journal: Eurosurveillance (2008)

We describe the first outbreak of non-typable methicillin-resistant Staphylococcus aureus on a surgical ward in the Netherlands in June 2007. Nine cases of infection and/or colonisation were found among patients and healthcare workers.
297. Development of HVJ-E High Throughput Gene Function Screening System: Screening of Endothelial Cell Growth Factors
Authors: Tomoyuki Nishikawa, Hironobu Nakagami, Chiah Yian Yo, Yasufumi Kaneda
Journal: Molecular Therapy (2004)

High Throughput screening system (HTS) is a powerful tool that can screen out gene function from pool of genes. Here, we developed a new HTS system using Hemagglutinating virus of Japan envelope (HVJ-E; Sendai virus) vector, which can be constructed by incorporating plasmid DNA into inactivated HVJ particles. After virus genome of HVJ is inactivated and broken down, the space inside of viral envelope is free to enclose variety of molecules such as pDNA, oligonucleotide, siRNA, antibody drug etc. In addition to the package ability, HVJ-E vector has wide range of target cells, both animal cells (especially primary and ES cells) and various tissues, by cell fusion activity. In this study, we screened endothelial growth genes to find out a new angiogenic factor. Human heart cDNA library (Gibco) was infused into HVJ-E vector and transfected to human aortic endothelial cells (HAEC; Clontech) that had been seeded into 96 well plates. For transtection, plasmid DNAs infused HVJ-E vectors were added to the medium of HAEC, and the plate was centrifuged for 1 hour. The plasmid DNA transfected HAEC were cultured in lower serum concentration medium (1% FBS) to reduce cell viability for seven days. We measured the HAEC viability by MTS assay. From the well of top score, DNA of cells were collected and transformed into E. coli to efficiently collect the candidate plasmids, because only plasmid DNAs, not cellular genomic DNAs, are formed in single clones (1st screening step of this method). We got couple thousands candidate genes from this step. All of the genes were sequenced, and homologies were searched by nucleotide-nucleotide BLAST (blastn). Based on the results of homologies, we have sorted genes that are secretion, signal transduction, transcription or unknown factors. The couple thousands of genes was narrow down to couple hundreds in this step. MTS assay were carried out again (2nd screening step of this method). Finally, we have isolated several novel genes that strongly promote HAEC growth. Further evaluation of newly found novel genes showed that they highly give rise to c-fos and VEGF promoter activation in endothelial cell. Overall, only two rounds of screening, 1st and 2nd screening, are sufficient to obtain target gene(s). In addition, all screened genes are retained in a plasmid form throughout whole screening steps, so that it saves much time and reduces chances of mutation during cloning procedures. HTS using HVJ-E vector will be a useful and powerful tool for screening the function of a lot of genes in four to six weeks Figure 1.
Rondine 5. März 62 g
Authors: M Therese Southgate
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 3680: (R,R')-4′-methoxy-1-naphtylfenoterol inhibits pro-survival signaling, proliferation and motility of select human melanoma cell lines
Authors: Artur Wnorowski, Rajib K. Paul, Lucita Jimenez, Lawrence Toll, Abdelmohsen Kotb, Michel Bernier, Irving W. Wainer
Journal: Cancer Research (2014)

Abstract (R,R')-4′-methoxy-1-naphtylfenoterol (MNF) inhibits proliferation of 1321N1 astrocytoma cell line in a β2-adrenergic receptor (β2AR)-dependent manner, while reducing rat C6 glioma growth in a xenograft mouse model. MNF interferes also with pro-oncogenic signaling induced by the orphan G-protein-linked receptor, GPR55, in human HepG2 hepatocarcinoma and PANC-1 pancreatic carcinoma cells. Here, we utilized a number of cellular and biochemical techniques to investigate the anti-tumorigenic potential of MNF in a panel of human melanoma cell lines. Cell migration, which was performed using scratch wound healing assay, demonstrated the ability of MNF to dose-dependently reduce the motility of EGFR-expressing UACC-647 and M93-047 cell lines in a biphasic (IC50 = 0.32 nM and 1.48 µM) and sigmoidal (IC50 = 7.14 nM) fashion, respectively. In contrast, EGFR-negative UACC-903 cells were unresponsive to MNF. The proliferation rate of UACC-647 cells was diminished by 38 ± 3.7% in the presence of MNF, with an IC50 of 136 nM. Pharmacological inhibition of β2AR with the selective antagonist ICI-118,551 (50 nM) did not confer protection against the actions of MNF on cell motility and proliferation. U-shaped dose-response curves, with a nadir at 1 nM, were observed when the levels of phosphorylated MEK and ERK were determined in MNF-treated UACC-647 cells. A similar dose-response relationship was observed with the β2AR agonists, epinephrine and (R,R')-fenoterol, and cell pretreatment with 50 nM ICI-118,551 abolished MNF-evoked drop in phospho-ERK levels. However, MNF markedly increased inhibitory phosphorylation of eukaryotic elongation factor 2 (eEF2) on Thr56, which was accompanied by global defect in protein biosynthesis as revealed by pulse-labeling experiments with [35S]-methionine. We conclude that the anti-tumorigenic functions of MNF most likely reflect dual effects of MNF through interaction with different receptors in melanoma. This work was supported entirely by the Intramural Research Program of the National Institute on Aging/NIH and NIA/NIH contract N01-AG31009, and the Foundation for Polish Science (TEAM 2009-4/5 programme). Citation Format: Artur Wnorowski, Rajib K. Paul, Lucita Jimenez, Lawrence Toll, Abdelmohsen Kotb, Michel Bernier, Irving W. Wainer. (R,R')-4′-methoxy-1-naphtylfenoterol inhibits pro-survival signaling, proliferation and motility of select human melanoma cell lines. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3680. doi:10.1158/1538-7445.AM2014-3680
Targeting FGF19 binding to its receptor system: A novel therapeutic approach for hepatocellular carcinoma
Authors: Nadia Panera, Sara Ceccarelli, Valerio Nobili, Anna Alisi
Journal: Hepatology (2015)

We carefully read a recent captivating article published in this journal.1 Ahn et al. depicted the possible genetic aberrations in resected hepatocellular carcinomas (HCCs) in order to evaluate the association between genome-wide traits and postoperative outcomes of patients. Because of different patients' features and/or sequencing tools, the authors found a considerable heterogeneity of gene mutations, even if some recurrent homozygous deletions and high copy amplifications were reported. Interestingly, RB1 mutation was associated with a negative prognostic significance after resection, while fibroblast growth factor 19 (FGF19) amplification correlated with cirrhosis in hepatitis B virus–related HCCs. This last finding highlights that HCCs characterized by somatic FGF19 amplification may represent a novel subtype of HCC that could respond to targeted therapy against FGF19 or its receptor system. Fibroblast growth factor 19 belongs to a subfamily of ligands which includes FGF21 and that regulates bile acid, fatty acid, and glucose metabolism in target organs, such as the liver. However, FGF19 and FGF21 require tissue-specific FGF receptors (FGFR1-4) and klotho-beta coreceptor for their functions.2 Interestingly, FGF21 was overexpressed in HCC.1, 3, 4 It should be very interesting to assess if, from the data set of genome-wide analysis, HCC samples in which FGF19 high copy amplifications overlap with copy number variations or single mutations localized at 19q13.33 containing the FGF21 gene may emerge. This could be very relevant to understanding if an anti-FGF19 strategy may be potentially sufficient as a therapeutic approach. In fact, Sawey et al. demonstrated the potential therapeutic benefit of targeting FGF19 with a neutralizing antibody in 11q13.3-amplified HCCs.5 In HCCs displaying FGF19 and FGF21 mutations alternative targeted strategies against the FGFR/klotho-beta receptor system could be more appropriate and efficient, even if this should be considered with caution because of the role of this system in hepatocyte homeostasis.6 Nadia Panera, Ph.D. Sara Ceccarelli, Ph.D. Valerio Nobili, M.D. Anna Alisi, Ph.D. Liver Research Unit Bambino Gesù Children's Hospital IRCCS Rome, Italy Author names in bold designate shared co-first authorship.
Oocyte apoptosis is suppressed by disruption of the acid sphingomyelinase gene or by sphingosine -1-phosphate therapy
Authors: Yutaka Morita, Gloria I. Perez, Francois Paris, Silvia R. Miranda, Desiree Ehleiter, Adrianna Haimovitz-Friedman, Zvi Fuks, Zhihua Xie, John C. Reed, Edward H. Schuchman, Richard N. Kolesnick, Jonathan L. Tilly
Journal: Nature Medicine (2000)
A memorable patient: A case of mistaken diagnosis
Authors: B. B Bhattacharya
Journal: BMJ (2001)

It was around May or June 1977. I had started my house job in psychiatry in India a couple of months before. Psychiatry was a brand new subject for me.

I was attending an outpatient clinic daily. The clinic was extremely busy. There were no set appointments (most of the patients did not have telephones at home), no distinct catchment area, and a referral letter from the general practitioner was not required. It was not surprising that a big crowd gathered in the clinic every morning seeking treatment. It was probably only the stigma of mental illness, which was more prevalent in those days, that prevented …
Most psychiatrists oppose plans for new mental health act
Authors: M J Crawford, W Hopkins, P Thomas, J Moncreiff, J Bindman, A J Gray
Journal: BMJ (2001)

EDITOR—In his editorial Szmukler expressed concerns about government plans to introduce legislation that would enable the preventative detention of people classified as having a dangerous severe personality disorder.1 We believe that most psychiatrists in Britain share these concerns.

This view is supported by the results of a survey that we conducted last year, which entailed sending a brief questionnaire to every consultant psychiatrist in England and Wales. The questionnaire …
Integrated Analysis of Whole-Exome Sequencing and Micrornas Expression in Blast Crisis Transformation of Chronic Myeloid Leukemia
Authors: Vera Magistroni, Nitesh Sharma, Rocco Piazza, Jason G Harb, Roberta Spinelli, Alessandra Pirola, Justin Ellis, Kim Dong-Wook, Danilo Perrotti, Carlo Gambacorti-Passerini
Journal: Blood (2012)
Newly available treatments for nicotine addiction
Authors: T. Coleman
Journal: BMJ (2001)

Smokers wanting help with stopping now have effective treatment options 

Nicotine addiction is recognised as a life threatening but treatable disorder1 and from 17 April, in accordance with the NHS Plan , all forms of nicotine replacement therapy were made available on NHS prescription.2 Britain now has a comprehensive treatment strategy for nicotine addiction, which includes provision of bupropion (Zyban) on prescription3 and the introduction of specialist smoking cessation services to provide behavioural support to people who want to stop smoking.4

In theory therefore, every smoker in the country who wants help with overcoming his or her addiction to nicotine now has access to effective treatments. Many general practitioners, however, are sceptical about the appropriateness of having nicotine replacement therapy or bupropion available on NHS prescription,5 and many are unaware of the part these can play in helping smokers to stop. The Health Development Agency is distributing a reprint of guidelines on smoking cessation …
THE UNUSUAL IN GASTROINTESTINAL ROENTGENOLOGY
Authors: G M TICE
Journal: Annals of Internal Medicine (1945)

Article1 November 1945THE UNUSUAL IN GASTROINTESTINAL ROENTGENOLOGYG. M. TICE, M.D.G. M. TICE, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-23-5-837 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptDuring the last 10 years there has been a marked increase in the number of qualified roentgenologists in this country.†The fact must be recognized that even with this group of specialists available, a large part of our population depends on internist, surgeon and general practitioner for diagnostic radiographic service. It is sincerely believed that the time will come and is not far distant when most cities of 20,000 population will support a qualified radiologist. In the meantime, where this service is not available or where the physician prefers to do his own diagnostic radiology, the greatest degree of skill...Bibliography1. RAIFORD TS: Tumors of the small intestine: their diagnosis with special reference to the x-ray appearance, Radiology, 1931, xvi, 253-279. CrossrefGoogle Scholar2. HERTZLER AE: Surgical pathology of diseases of the gastrointestinal tract, 1936, J. B. Lippincott, Philadelphia, p. 207. Google Scholar3. ABEL I: Acute abdominal catastrophes, Jr. Am. Med. Assoc., 1937, cix, 1241-1245. (GOLDSTEIN in discussing paper by ABEL.) CrossrefGoogle Scholar4. DALZIEL TK: Chronic interstitial enteritis, Brit. Med. Jr., 1913, ii, 1068-1070. Google Scholar5. CROHNGINZBURGOPPENHEIMER BBLGD: Regional ileitis, a pathological and clinical entity, Jr. Am. Med. Assoc., 1932, xcix, 1323-1329. CrossrefGoogle Scholar6. HARRISBELLBRUNN FIGHH: Chronic cicatrizing enteritis. Regional enteritis, a new surgical entity, Surg., Gynec., and Obst., 1933, lvii, 637-645. Google Scholar7. CARRBOECK PWC: Chronic ulcerative enteritis, Am. Jr. Digest. Dis. and Nutr., 1934, i, 161-163. CrossrefGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Kansas City, Kansas*Received for publication March 13, 1945.†In 1930 there were 1,005 radiologists in the United States. In 1940, 2,866 physicians stated that they were radiologists.—Information from American College of Radiology. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 November 1945Volume 23, Issue 5Page: 837-851KeywordsDiagnostic radiologyRadiologistsSurgeons Issue Published: 1 November 1945 PDF downloadLoading ...
The measurement of constitutive and facultative skin pigmentation and estimation of sun exposure in caucasians with basal cell carcinoma and cutaneous malignant melanoma
Authors: None Lock-Andersen, None Drzewiecki, None Wulf
Journal: British Journal of Dermatology (1998)

In two identical and simultaneously performed case-control studies of basal cell carcinoma (BCC) and cutaneous malignant melanoma (CMM) with age-matched, sex-matched and residence-matched controls, skin pigmentation was measured objectively by skin reflectance spectroscopy in 145 BCC patients and 174 matched controls and in 168 CMM patients and 176 matched controls. Measurements were performed at the forehead, the upper chest, the upper back, the lateral and medial aspects of the upper arm, and the buttocks. Self-estimation of sun exposure in childhood, in youth and in adulthood was performed by all subjects. There were no statistically significant differences in constitutive skin pigmentation at the buttocks between BCC patients and controls (P = 0.96) or between CMM patients and controls (P = 0.13). Facultative skin pigmentation in ultraviolet-exposed sites was not significantly different between BCC patients and controls except that women patients had higher pigmentation at the lateral side of the upper arm. For CMM, men patients had higher pigmentation at the lateral side of the upper arm. Self-estimations of sun exposure did not show differences between patients and controls but indicated high exposure levels in childhood and youth and in adult leisure time. Sun exposure estimated by increase in facultative pigmentation above the constitutive level (the Sun Exposure Index) was not significantly different between BCC patients and controls, whereas CMM men patients had higher estimates for the lateral side of the upper arm, the chest and the back.