tbuckley/cabot-search huggingface dataset:
Abstract 1473: Indentification of microRNA signature and deregulation in breast, lung and colon cancer through next generation sequencing and quantitative reverse transcriptase PCR
Authors: Chunmei Liu
Journal: Cancer Research (2014)

Abstract Global alterations of microRNA expression have been described in a large number of malignancies and microRNA signatures have been used as classifier for diagnosis, prognosis and therapeutic response in cancer. In current study, we compared the global patterns of microRNA expression in breast cancer, lung cancer and colon cancer patients with their normal adjacent tissues through next generation sequencing (NGS). Differentially expressed microRNAs such as let-7 family, miR34 family, miR21 and miR29 family, miR17, miR92a, miR205, miR145 as well as other signature microRNAs were further validated through quantitative reverse transcriptase PCR (qRT-PCR). Since microRNAs do not function in isolation, we also compared the expression level of possible targeted mRNAs such as target s for Let-7 family, HMGA2 (high mobility group AT-hook2) , RAS, KRAS; targets for miR-34, SNAIL(zinc finger protein) , Wnt1, SIRT1 and CDK6; targets for miR-21 , tropomyosin (TPM1), PDCD4 and PTEN; targets for MiR29 family, MCL1, KLF4 through qRT-PCR. Note: This abstract was not presented at the meeting. Citation Format: Chunmei Liu. Indentification of microRNA signature and deregulation in breast, lung and colon cancer through next generation sequencing and quantitative reverse transcriptase PCR. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1473. doi:10.1158/1538-7445.AM2014-1473
Abstract 20056: The Correlation Between Coronary Plaque Burden Assessed by Cardiac Computed Tomography and Serum Levels of Cardiac Troponin T
Authors: Johannes Rixe, Joerg Wilhelm, Helge Moellmann, Holger Nef, Christoph Liebetrau, Sebastian Szardien, Damir Erkapic, Thorsten Dill, Christian W. Hamm, Andreas Rolf
Journal: Circulation (2010)

Background:  High sensitive cardiac Troponin T (cTnT) is a predictor of death in patients with stable coronary artery disease (CAD). Thus, it might be an indicator of coronary plaque instability in patients with subclinical atherosclerosis. We sought to investigate the correlation between coronary plaque burden assessed by Computed Tomography (CT) and the serum levels of cTnT quantified by a conventional and a high sensitive assay.   Methods:  95 patients (74 male, mean age 59±11y) underwent Dual Source-CT (Siemens Somatom Definition) for the exclusion of CAD. Before scan, serum levels of cTnT were determined with a conventional (lower detection threshold 0.001 ng/ml) and a high-sensitive assay (lower detection limit 3 pg/ml). CT data sets were assessed with a dedicated software (Siemens Circulation) for presence and quantity of coronary plaques.   Results:  Serum levels are given in the table below. For both assays, presence of coronary plaque lead to elevated serum levels of cTnT. Moreover, in patients with > 1 coronary plaque the highest serum levels of cTnT could be determined. However, this was statistically significant only for the high-sensitive assay (p=0.05 for pts with plaque vs. pts without and 0.03 for pts with > 1 plaque vs. pts without), whereas there was no statistical significance for comparison of any plaque subgroup if measured with the conventional assay (p=0.15 for pts with plaque vs. pts without and 0.1 for pts with > 1 plaque vs. pts without, respectively). Furthermore, patients with only one coronary plaque did not have elevated serum levels of cTnT if compared to those without, irrespective of the assay used.   Conclusion:  The presence of coronary plaque is related to a significant increase of cTnT serum levels, if a high-sensitive immunoassay is used. However, there is no consistent elevation of cTnT in presence of coronary plaque in general, putting the the role of cardiac TnT for the detection of coronary plaque into perspective.
Abstract 2862: Overexpression of nuclear karyopherin alpha2 correlates with poor prognosis in patients with colorectal cancer
Authors: Moo Jun Baek, Dongjun Jeong, Tae Hyun Kim, Chang-Jin Kim, Sang Han Lee, Han J. Kim, Sang Byeong Bae, Tae Sung Ahn
Journal: Cancer Research (2014)

Abstract Objectives: Karyopherin alpha 2 (KPNA2) is a member of the karyopherin family that moves protein molecules into the nucleus by binding to a specific recognition sequence. It has recently been reported to play an important role in carcinogenesis and cancer progression. The aim of the present study was to elucidate the clinicopathological significance of overexpression of nuclear KPNA2 expression in colorectal cancers. Methods: KPNA2 expression was investigated by immunohistochemistry on tissue microarrays of 363 cases of colorectal cancer and the association of KPNA2 expression was examined with clinicopathologic features. Results: Thirty percent (30%) of colorectal cancers demonstrated overexpression of KPNA2. The overexpression of KPNA2 was significantly associated with high pN stages (p=0.005), vascular invasions (p&amp;lt;0.001) and lymphatic invasions (p&amp;lt;0.001). Multivariate Cox regression analysis showed that the overexpression of PKNA2 in pN stage 1, 2 was an independent prognostic factor of decreased survival [HR=2.13 (95% CI: 1.32-3.45, p=0.002)]. The overall survival was significantly decreased in patients of KPNA2 high expression [HR=2.70 (95% CI: 1.74-4.20, p&amp;lt;0.001)]. The cumulative survival was significantly decreased in patients of KPNA2 high expression compared to those of KPNA2 low expression by Kaplan-Meier analysis (log rank test, p&amp;lt;0.001). Conclusion: The overexpression of KPNA2 can be a valuable poor prognostic marker of colorectal cancer and the KPNA2 can be used in therapeutic strategies. Citation Format: Moo Jun Baek, Dongjun Jeong, Tae Hyun Kim, Chang-Jin Kim, Sang Han Lee, Han Jo Kim, Sang Byeong Bae, Tae Sung Ahn. Overexpression of nuclear karyopherin alpha2 correlates with poor prognosis in patients with colorectal cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2862. doi:10.1158/1538-7445.AM2014-2862
Electronic Documentation of Lifestyle Counseling and Glycemic Control in Patients With Diabetes
Authors: Naoshi Hosomura, Saveli I. Goldberg, Maria Shubina, Mary Zhang, Alexander Turchin
Journal: Diabetes Care (2015)

OBJECTIVE To establish quantitative characteristics of lifestyle counseling documentation associated with improved glycemic control in patients with diabetes. RESEARCH DESIGN AND METHODS We retrospectively studied 10,870 hyperglycemic (HbA1c ≥7.0% [53 mmol/mol]) adults with diabetes followed at primary care practices affiliated with two academic hospitals between 2000 and 2010. Documentation intensity was represented by the mean number of characters per note documenting lifestyle counseling. Heterogeneity was calculated as the normalized Levenshtein distance between lifestyle counseling sentences between consecutive notes. Cox proportional hazards model was constructed to assess association of heterogeneity and intensity of lifestyle counseling documentation to time to HbA1c &amp;lt;7.0% (53 mmol/mol) while adjusting for demographics, initial HbA1c level, insulin therapy, medication intensification, and frequency of lifestyle counseling. RESULTS Comparing patients in the highest versus lowest tertile by documentation heterogeneity and documentation intensity, median time to HbA1c &amp;lt;7.0% (53 mmol/mol) was 26 vs. 39 months and 24 vs. 39 months, respectively (P &amp;lt; 0.001 for all). In multivariable analysis, an increase of documentation heterogeneity by 0.15 units and an increase of documentation intensity by 45 characters/note was associated with hazard ratios of 1.08 (95% CI 1.04–1.12; P &amp;lt; 0.001) and 1.27 (95% CI 1.23–1.31; P &amp;lt; 0.001) for time to HbA1c target, respectively. CONCLUSIONS Higher heterogeneity and intensity of lifestyle counseling documentation in provider notes were associated with better glycemic control. Further studies involving direct observation of patient care are needed to establish the nature of the relationship between documentation characteristics and patient outcomes.
Abstract 60: Higher Fitness is Associated with Lower Health Care Costs: The Veterans Exercise Testing Study
Authors: Jonathan Myers, Robert King, Holly Fonda, Joshua Abella, Victor Froelicher, Peter Kokkinos
Journal: Circulation (2014)

Introduction: The association between poor physical fitness and adverse health outcomes is well-established, but few data are available regarding the association between fitness and health care costs. Hypothesis: Higher fitness is associated with lower overall health care costs. Methods: We studied 1,294 patients (mean 64±12 years) who underwent maximal exercise testing for clinical reasons as part of the Veterans Exercise Testing Study (VETS). Fitness was expressed as the percentage of age-predicted peak METs achieved, and categorized in quartiles. Total and annualized health care costs, derived from the VA Allocated Resource Center, were assessed between 2005 and 2010. Health care costs between quartiles of fitness were compared using ANOVA; multiple regression was used to determine clinical and exercise test predictors of health care costs. Follow-up for all-cause mortality (mean 8.5±5 years) was performed through March 2013. Results: A gradient for reduced health care costs was observed with increased fitness, with subjects in the least-fit quartile having approximately twice the overall costs as those in the fittest quartile (Table). Non-survivors were significantly less fit (6.5±5.1 vs. 9.1±3.5 METs, p&lt;0.001) and exhibited roughly 3 times the health care costs of those who survived. In a multivariate model including historical, clinical and exercise test responses, fitness was a significant predictor of health care costs (p&lt;0.01). Conclusions: Low fitness is associated with a significant burden on the health care system. Improving fitness should be encouraged for its potential to lower health care costs.
Ibrutinib In Combination With Bendamustine and Rituximab Is Active and Tolerable In Patients With Relapsed/Refractory CLL/SLL: Final Results Of a Phase 1b Study
Authors: Jennifer R Brown, Jaqueline C. Barrientos, Paul M. Barr, Ian Flinn, Jan A. Burger, Zeena Salman, Fong Clow, Danelle F. James, Thorsten Graef, Jonathan W. Friedberg, Kanti R. Rai, Susan O'Brien
Journal: Blood (2013)
Abstract 1517: Does Angiogram Make A Difference In The Outcome Of Acute Coronary Syndrome? A Study On Sex Difference In Outcomes In South America.
Authors: Raffaele Bugiardini, Florencia Rolandi, Oscar Bazzino, Olivia Manfrini, Andres Pascua, Fernando Botto, Juan Fuselli, Carlos Rojas Matas, Jose L Navarro Estrada
Journal: Circulation (2007)

HYPOTHESIS. Women presenting with acute coronary syndrome are less likely to have significant coronary artery disease (CAD) than men, which could narrow wide differences in sex outcomes when evaluating the study population as a whole. METHODS. The Prognosis in Acute Coronary Syndromes Registry enrolled 823 patients (591 men and 232 women) who had been hospitalized for an acute coronary syndrome without ST-segment elevation and had undergone cardiac catheterization. We explored sex-based differences in presentation and outcomes, sorted by angiographic groups: obstructive (≥50% stenosis, accordingly to quantitative computerized analysis) versus non-obstructive CAD. Patients were followed up for 6 months. RESULTS. In obstructive CAD, women were older than men (71.4 ± 9.7 versus 64.4 ± 11.1 years, p&lt;0.001), and had significantly higher rates of hypertension (51.9% versus 39.7%, p&lt;0.001). Women were less likely to have smoked (19.3% versus 29.8%, p&lt;0.01). A smaller percentage of women than men had non-ST elevation myocardial infarction as an index event (7.7% versus 22.8%, p&lt;0.001) and positive troponin value (51.3% versus 67.4%, p&lt;0.01). At follow-up women showed no differences in myocardial infarction, rehospitalization for unstable angina or revascularization, but they did suffer an increased rate of cardiovascular death (8.4% versus 3.4%, p&lt;0.01), with a hazard ratio 2.34 (95%CI: 1.13– 4.84, p=0.023). Relation between sex and death remained significant even after adjustment for any confounders (hazard ratio 2.48; 95%CI: 1.19–5.15, p=0.015). In non-obstructive CAD group, the clinical characteristics and prognostic end-points (death: 0% men versus 1% women) did not significantly differ between men and women. CONCLUSIONS. In conclusion, women with obstructive CAD suffer an increased rate of cardiovascular death after acute coronary syndrome. Inclusion of large numbers of women with non-obstructive coronary disease in calculations based on the entire cohort may mistakenly shift results toward apparent outcome similarity with men.
Abstract 11964: A Fourth Generation Telehealth Care Program is Associated With Lower Hospitalization Rate, Duration and Lower Medical Cost
Authors: Chi Sheng Hung, Ying-Hsien Chen, Ching-Chang Huang, Deng-Feng Yeih, Yen-Hung Lin, Yi-Lwun Ho, Ming-Fong Chen
Journal: Circulation (2013)

Background: Telehealth management program is a rapid growing field for the care of patients with chronic diseases. The fourth generation of telemedicine involved a synchronous data transfer and ful...
Abstract 4932: CXCR4 Expression on Bone Marrow-derived Cells Was Regulated Positively by Calcium and Negatively by Aging
Authors: Qiyuan Xu, Qiuling Wu, Hongwei Shao, Jiahui Li, Qi Ma, Darwin Eton, Keith A Webster, Hong Yu
Journal: Circulation (2009)

Cell surface receptors play major roles in the mobilization and homing of progenitor cells from the bone marrow to peripheral tissues. CXCR4 is an important receptor that regulates homing of leukocytes and vascular progenitor cells (VPC) in response to the chemokine stromal cell-derived factor-1 (SDF-1). Ionic calcium is also known to regulate chemotaxis of selective bone marrow-derived cells (BMC) through the calcium-sensing receptor CaR. We assessed the hypothesis that calcium regulates CXCR4 expression on BMC; defective CXCR4 expression develops with aging, which results in less VPC homing to vascular lesion site and inefficient repair. We found that CXCR4 surface expression is age-dependent. CXCR4 on the surface of BMC from old mice (24 months old) is significantly lower than young mice. We also found that CaCl 2 treatment of BMC from young mice induced a significant increase in both the transcription and cell surface expression of CXCR4. BMC subpopulations expressing VEGFR2 + and CD34 + , which were considered as endothelial progenitor cells, were especially sensitive to calcium. The effects were blocked by calcium influx inhibitors, anti-CaR antibody, and the protein synthesis inhibitor cycloheximide. Calcium treatment also enhanced SDF-1-mediated CXCR4 internalization. These changes were reflected in significantly improved chemotaxis by SDF-1, which was abolished by the CXCR4 antagonist AMD3100 and by antibody against CXCR4. Calcium pre-treatment improved homing of CD34 + BMC to ischemic muscle in vivo, and enhanced revascularization in ischemic mouse hindlimbs. Such calcium-mediated increase of surface CXCR4 was not observed on BMC from old mice. Pre-treatment of BMC from old mice with calcium did not significantly improve the cellular signaling response to SDF-1, nor enhance homing of injected BMC to ischemic muscle and blood reperfusion in vivo. In conclusion, we identified calcium as a positive regulator of CXCR4 expression that enhances progenitor cell mobility and efficiency of cell-based therapy. Aging negatively affects BMC CXCR4 expression and its interaction with SDF-1. This research has received full or partial funding support from the American Heart Association, Greater Southeast Affiliate (Alabama, Florida, Georgia, Louisiana, Mississippi, Puerto Rico &amp; Tennessee).
Major depression in a community sample of African Americans
Authors: D R Brown, F Ahmed, L E Gary, N G Milburn
Journal: American Journal of Psychiatry (1995)

This study examined demographic, sociocultural, familial background, and health-related risk factors for major depression in a community sample of African Americans.Data came from a probability sample of 865 urban African American adults, 20 years of age and older, who were given the structured National Institute of Mental Health Diagnostic Interview Schedule.The findings indicated a 1-year prevalence of 3.1% for major depression. While age, residential mobility, health status, and stressful life events were significantly associated with major depression, none of the sociocultural and family background factors were. The strongest predictors of major depression were poor or fair health and being 20-29 years of age. In terms of treatment for depression, persons with major depression were significantly more likely than those without to engage in help seeking. However, only 11.1% actually saw a psychiatrist or other mental health professional, and 7.4% saw no one.Young age and fair to poor physical health appear to be more powerful risk factors for major depression among African Americans than other demographic, sociocultural, and family background variables. Few African Americans with major depression actually receive clinical treatment for this illness. The results further suggest the need to increase awareness of major depression among general practitioners and to target outreach to African American communities.
Sleep clinical record: To screen and follow children with obstructive sleep apnea
Authors: Ottavio Vitelli, Anna Sujanska, Luca Brasili, Maria Chiara Supino, Chiara Romano, Peter Banovcin, Maria Pia Villa
Journal: European Respiratory Journal (2013)

Background : Our group has previously validated the Sleep Clinical Record (SCS) to screen patients candidates to perform polysomnography (PSG) for suspected obstructive sleep apnea.

Aim : To assess the utility of SCS as a follow up instrument after treatment.

Methods: Every patients underwent PSG and the SCR was fulfilled. The SCR consists of 3 points: physical examination, subjective symptoms and clinical history, behavioural and cognitive problems. A second PSG and SCR were made after the recommended treatment for each child (local therapy, adenotonsillectomy, orthodontic treatment or adenotomy).

Results: We studied 48 children, mean age 4,7 ± 1,4 years, 52% males. At baseline apnoea hyponea index (AHI) (14.03±10.46 ev/h) and SCR (8,27±2,1) showed a positive correlation (p< 0,05). A positive correlation (p< 0,05) persists even after the treatment between AHI (3.15±3.95 ev/h) and SCR (4.82±1.92).

Conclusions: The SCR is a good paediatric instrument to evaluate the improvement of obstructive sleep apnea after treatment.
Abstract 1987: Molecular characterization of the breast cancer-associated antigen NY-BR-1
Authors: Julia Bitzer, Zeynep Kosaloglu, Niels Halama, Claudia Ziegelmeier, Tina Lerchl, Anna Spille, Maria Pudenz, Eva Koellensperger, Stefan Eichmueller, Wolfram Osen, Andreas Schneeweiss, Frederik Marmé, Inka Zoernig, Dirk Jaeger
Journal: Cancer Research (2015)

Abstract Breast cancer is one of the most common malignancies with increasing incidence every year and a leading cause of death among women. Although early stage breast cancer can be effectively treated, there are limited numbers of treatment options available for patients with advanced and metastatic disease. The breast cancer associated antigen NY-BR-1 was identified by a serological screening strategy (SEREX). NY-BR-1 is expressed in the majority (&amp;gt;70%) of breast tumors as well as metastases, in normal breast tissue, in testis, and occasionally in prostate tissue. NY-BR-1 was confirmed as an immunogenic antigen as we could detect NY-BR-1 specific spontaneous humoral and cellular immune responses in several breast cancer patients. However, biological function, regulatory mechanisms, and interaction partners of NY-BR-1 are still unknown. Therefore, we combined integrative functional analysis (genomics, transcriptomics and epigenetics) and experiments to unravel the transcriptional regulation of NY-BR-1 and to detect its function within the mammary gland. As numerous binding sites for nuclear hormone receptors (e.g. Estrogen Receptor alpha, Progesterone receptor and Glucocorticoid receptor) and CpG islands were predicted within the promoter sequence of NY-BR-1, primary-tissues from breast reductions or tumor cells obtained from pleural effusions were treated with different hormones (Estradiol, Dexamethasone, Progesterone, Retinoic acid, Vitamin D) or with the HDAC inhibitors Valproic acid, Trichostatin A and the demethylating agent 5´Aza-Deoxycytidine in order to analyze the transcriptional regulation of NY-BR-1. Additionally, we analyzed the methylation status at various sites within the promoter region of NY-BR-1 using EpiTyper MassARRAY technology and publicly available resources such as the TCGA database. Here, we present preliminary results of our analysis and evaluate their implication for further investigation of NY-BR-1. Citation Format: Julia Bitzer, Zeynep Kosaloglu, Niels Halama, Claudia Ziegelmeier, Tina Lerchl, Anna Spille, Maria Pudenz, Eva Koellensperger, Stefan Eichmueller, Wolfram Osen, Andreas Schneeweiss, Frederik Marmé, Inka Zoernig, Dirk Jaeger. Molecular characterization of the breast cancer-associated antigen NY-BR-1. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 1987. doi:10.1158/1538-7445.AM2015-1987
Temperature of pool is important
Authors: A. C Deans, P. J Steer
Journal: BMJ (1995)

was screened by a nurse (and possibly many fewer if patient self screening was feasible) this would cost £4000 in a practice of four partners, in which 8-16 melanomas would be diagnosed over 10 years.If one of these melanomas could be prevented or diagnosed earlier, and other costs minimised, 15-20 life years could be saved with a cost effectiveness comparable with screening for that of hypertension.Although Keeley reinforces some valid points, he ignores suitable study design, automatically assumes pessimistic outcomes, and unnecessarily polarises the debate when research is at such an early stage.