tbuckley/cabot-search huggingface dataset:
498. Targeted Gene Transfer to Lung Airway Epithelium Using Plasmid or Adenoviral Vectors Formulated with an Anti-Inflammatory Dexamethasone-Spermine Conjugate
Authors: Amber Price, Maria Limberis, Jeffrey Gruneich, James M. Wilson, Scott L. Diamond
Journal: Molecular Therapy (2004)

Introduction: Gene transfer to the airway epithelium for the treatment of airway diseases such as cystic fibrosis and alpha-1-antitrypsin deficiency has been limited by significant inflammatory responses towards plasmid DNA (pDNA), lipoplexes, and viral vectors. Cationic dexamethasone-spermine (DS) may help improve targeting of AdV vectors to the airways and deliver sustained local glucocorticoid (GC) activity. The potent anti-inflammatory effects of GC's suggest that the GC activity of DS could also reduce the inflammation associated with delivering gene therapy components in vivo
Abstract 5734: Left Ventricular Torsion Reflects Degree of Myocardial Fibrosis in Hypertrophic Cardiomyopathy
Authors: Ayumi Nakaboh, Akiko Goda, Shinji Nakao, Katsumi Oka, Takeshi Tsujino, Masao Yuba, Misato Otsuka, Mika Matsumoto, Chikako Yoshida, Yoshiro Naito, Masaaki Lee-Kawabata, Tohru Masuyama
Journal: Circulation (2008)

Background : LV torsion (LVtor) is reduced in patients with impaired LV systolic function, e.g., dilated cardiomyophaty. It used to be thought that LVtor is reduced in patients with hypertrophic caridiomyopathy (HCM) because of the myocardial disarray; however, recent observation showed that LVtor assessed with tagged magnetic resonance imaging (MRI) even increased in patients with HCM. Recently LVtor is assessable with novel ultrasound speckle tracking imaging (STI) method. This technique may be used to assess intra-ventricular torsion in the LV muscle (intra-LVtor) that occurs due to the different fiber directions between the endocardium and epicardium. In this study, we analyzed LVtor and intra-LVtor in light of LV fibrosis in patients with HCM. Methods : MRI and echo studies were performed in 10 patients with HCM in whom any medication had not been given to HCM. MRI was used to determine LV mass index. In addition, %DEmass was determined as an MRI index of the extent of myocardial fibrosis by dividing the extent of late gadolinium enhancement by LV mass. We acquired basal and apical short-axis LV images with 2D echocardiography for off-line STI analysis to determine LVtor and intra-LVtor. We traced 18 points of the endcardium and epicardium, respectively, and averaged all regional rotation at the basal and apical plane, respectively. LVtor was defined as the difference of LV rotation between the basal and apical plane. Intra-LVtor was defined as the difference in the rotation between the endcardium and epicardium. Results : There was a significant correlation between %DEmass and LVtor(r=−0.72, p&lt;0.05, indicating that LVtor is reduced in HCM patients with larger extent of LV myocardial fibrosis. There was no significant correlation between LVtor and LV mass index (r=−0.15, p=n.s.). %DEmass also correlated with intra-LVtor (r=−0.67, p&lt;0.05, indicating that intra-LVtor is reduced in HCM patients with larger extent of LV myocardial fibrosis. Conclusions : Both LV torsion and intra-LV torsion are reduced in HCM patients with extended LV fibrosis. In other words, the extent of myocardial fibrosis may be assessed with LVtor and intra-LVtor by use of speckle tracking imaging in patients with HCM.
Cerebral blood flow in depression
Authors: R J Mathew, J S Meyer, D J Francis, K M Semchuk, K Mortel, J L Claghorn
Journal: American Journal of Psychiatry (1980)

This section contains 1) new research findings, including preliminary data from pilot studies, either clinical or laboratory; 2) worthwhile replication studies; 3) case reports that describe a truly new syndrome or cast new light on established ones; and 4) case reports that indicate a new therapeutic procedure of potential value or call attention to adverse effects of drugs or previously unreported complications of therapeutic interventions. Program descriptions and literature reviews cannot be printed in this section. Criteria for format are listed in "Information for Contributors" in each issue; papers that do not adhere to these criteria will be returned to the author.
750. AAV-2 Vectors and Protease-Activated Receptors: A Potential Interface between Coagulation and Gene Transfer
Authors: Jianxiang Zou, Joerg Schuettrumpf, Alexander Schalchterman, Kian W. Tian, Jian-Hua Liu, Patricia Andrade-Gordon, Valder R. Arruda
Journal: Molecular Therapy (2004)
Soybean allergy in patients allergic to birch pollen☆Clinical investigation and molecular characterization of allergens
Authors: Diana Mittag, Stefan Vieths, Lothar Vogel, Wolf-Meinhard Becker, Hans-Peter Rihs, Arthur Helbling, Brunello Wüthrich, Barbara K. Ballmer-Weber
Journal: Journal of Allergy and Clinical Immunology (2004)
Abstract P202: Is There a Relationship Between the Psychological Influence of the Highly Palatable Food Environment and Weight Loss Attempts?
Authors: Molly Matthews-Ewald, Phillip J Brantley, Melissa N Harris, Valerie Myers, Robert Newton, Catherine Champagne
Journal: Circulation (2014)

Background: Research suggests that the degree of food desirability may have an impact on successful weight loss. Defined as susceptibility to eating when presented with environmental stimuli, hedonic hunger is related to increased food consumption. Individuals with higher hedonic hunger may be less able to successfully maintain weight loss and, therefore, may have more weight loss attempts (WLA). The purpose was to examine associations between the number of WLA and the PFS total scale and subscale scores, controlling for race, sex, BMI, and age. Methods: Participants were obese adults enrolled in Heads Up, an insurance-sponsored observational study examining surgical and non-surgical weight loss techniques. Individuals completed the Power of Food Scale (PFS) and demographic information, including the number of WLA. The PFS was developed to assess hedonic hunger when food is: 1) available, 2) present, and 3) tasted. Results: Of the 705 participants, 409 (57.8%) were Caucasian, 597 (84.3%) were female, and had attempted to lose weight 9.13 (SD=9.8) times. The number of WLA significantly predicted PFS total scores and subscale scores except for the “Food Tasted” subscale. The full linear regression models accounted for 4.7%, 4.1%, and 6.2% of the variance in the total PFS, Food Available subscale, and Food Present subscale scores, respectively. Conclusions: Results demonstrate that hedonic hunger may be a factor in repeated WLA. Future research should examine the temporal sequence to fully explain this relationship to provide additional tailoring of behavioral weight loss interventions to address hedonic hunger as a hindrance to successful weight maintenance.
Not Breathing Easier With the US FDA’s Ban on Chlorofluorocarbons in Inhalers
Authors: Rita F. Redberg
Journal: JAMA Internal Medicine (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Internal Medicine HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Internal Medicine journal
Analysis of human leukemias using extensive immunophenotypes from an antibody microarray
Authors: Richard I. Christopherson, Larissa Belov, Stephen P. Mulligan, Nicole Barber, Adrian Woolfson, Mike Scott, Kerryn Stoner, Jeremy S. Chrisp, William A. Sewell, Kenneth F. Bradstock, Linda Bendall, Dana S. Pascovici, Mervyn Thomas, Wendy Erber, Graham Ar Young, James S. Wiley, Surender Juneja, William G. Wierda, Anthony R. Green, Michael J. Keating
Journal: Clinical Cancer Research (2006)

A12   A novel microarray containing 82 cluster of differentiation (CD) antibodies has been used to obtain extensive immunophenotypes of leukemia cells. Leukocytes are captured on antibody dots corresponding to particular cell surface molecules to give a dot pattern that is the immunophenotype. Samples from the peripheral blood and bone marrow of 733 leukemia patients were analyzed. Discriminant Function Analysis of the expression profiles from these 733 patients and 63 normal subjects were clustered and showed high levels of consistency with diagnoses obtained using conventional criteria. The overall levels of consensus for classification using the microarray compared with established criteria were 93.9% (495/527 patients) for peripheral blood and 97.6% (201/206 patients) for bone marrow aspirates, showing that the extensive phenotype alone was frequently able to classify the disease when the leukaemic clone was the dominant cell population. Immunophenotypes for neoplastic cells were distinguishable from normal cells when the leukaemic count was at least 5 X 10(9) cells/L in peripheral blood, or 20% of cells obtained from bone marrow aspirates. This technique will be a useful adjunct to flow cytometry and other methods when an extensive immunophenotype of leukemia cells is required. An extensive immunophenotype alone may be sufficient for diagnosis of most leukemias.
728. Baculovirus GP64 Pseudotyped Bovine Immunodeficiency Virus-Based Lentiviral Vectors Efficiently Transduce Retinal Cells In Vivo
Authors: Rene P. Molina, Hongqing Q. Ye, James Brady, Christine A. Mech, Michael Kaleko, Tianci Luo
Journal: Molecular Therapy (2004)

Pseudotyping lentiviral vectors with heterlogous viral envelopes has broadened the tropism of the vectors and made the viral particles more stable. Vesicular stomatitis virus glycoprotein (VSV-G) has been widely used for pseudotyping various lentiviral vectors because its broad tropism. However, VSV-G is cytotoxic, making it problematic to generate a packaging cell line. Progress has been made recently in finding alternative pseudotyping envelopes to VSV-G. Baculovirus GP64 has been shown to efficiently pseudotype human immunodeficiency virus (HIV) based vector. Unlike VSV-G, Baculovirus GP64 is not cytotoxic and can be constitutively expressed in human cells. In this report, we generated Baculovirus GP64 pseudotyped bovine immunodeficiency virus (BIV) based vectors and tested the vectors in vitro and in vivo. The results show that (1) baculovirus GP64 pseudotyped BIV vectors are stable and achieve similar titer as VSV-G pseudotyped vectors, (2) the vectors efficiently transduce non-dividing cells in vitro and rat retinal pigment epithelial cells in vivo. Our results suggest the baculovirus pseudotyped BIV vectors are useful for ocular gene therapy with retinal epithelial cells as targets.
Prevalence of smoking among psychiatric outpatients
Authors: John R. Hughes, Dorothy K. Hatsukami, James E. Mitchell, Lisa A. Dahlgren
Journal: American Journal of Psychiatry (1986)

The prevalence of smoking among psychiatric outpatients (N = 277) was significantly higher than among either local or national population-based samples (N = 1,440 and 17,000) (52% versus 30% and 33%). The higher prevalence was not associated with the age, sex, marital status, socioeconomic status, alcohol use, coffee use, or institutionalization of the psychiatric patients. Smoking was especially prevalent among patients with schizophrenia (88%) or mania (70%) and among the more severely ill patients. Hypotheses about why psychiatric patients are more likely to smoke and why they do not have a high rate of smoking-induced illnesses are presented.
Abstract 2594: Adjuvant cationic nanoliposomes induce anti-cancer immunity in a murine model of breast cancer
Authors: Ismail M. Meraz, David J. Savage, Jianhua Gu, Jessica Rhudy, Rita E. Serda
Journal: Cancer Research (2014)

Abstract Nanoparticles, such as liposomes, provide opportunities to simultaneously present antigens and immune modulators. Using a 4T1 murine model of breast cancer, a cationic nanoliposomal formulation containing monophosphoryl lipid A and the cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane induced anti-tumor activity following intratumoral administration. Addition of recombinant interleukin-12 (IL-12) further suppressed tumor growth and augmented T helper-1 cell (Th-1) polarization, with enhanced tumor infiltration by cytotoxic T cells, dendritic cells, and M1 macrophages, and amplification of interferon gamma secretion. Mice bearing dual tumors displayed arrest of tumor growth in treated tumors as well as distal, untreated tumors following combination therapy with adjuvant nanoliposomes and IL-12. In summary, adjuvant MPL-liposomes combined with localized IL-12 therapy block tumor growth, stimulate a Th-1 bias of the tumor microenvironment, and induce cancer-specific immune responses. Citation Format: Ismail M. Meraz, David J. Savage, Jianhua Gu, Jessica Rhudy, Rita E. Serda. Adjuvant cationic nanoliposomes induce anti-cancer immunity in a murine model of breast cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2594. doi:10.1158/1538-7445.AM2014-2594
A Novel Mechanism of Synergistic Cytoxicity between Prodrugs, 5-FC and GCV in Double Suicide Gene Therapy
Authors: Paul D. Boucher, Jessica O'Konek, Svend O. Freytag, Donna S. Shewach
Journal: Molecular Therapy (2004)

The simultaneous use of CD/5-FC and HSV-TK/GCV suicide gene protocols has resulted in enhanced antitumor activity in cultured tumor cell lines as well as in mouse models. It has been suggested that the CD/5-FC-mediated decrease in dTTP leads to lower thymidine levels and decreased competition for GCV phosphorylation by HSV-TK. In this study, we demonstrate that concurrent addition of prodrugs, 5-FC and GCV, was less efficacious than sequential addition of 5-FC followed by GCV treatment in human DU145 prostate carcinoma cells infected with an adenovirus containing a CD/HSV-TK fusion gene. The level of radiolabeled GCV-TP in cells treated simultaneously with 200 μg/ml 5-FC and 1.0μM GCV for 24 hr was 188 pmol/106 cells compared to 143 pmol/106 cells in cells treated with GCV alone. However, if the cells were pretreated 24 hr with 5-FC and then incubated 24 hr with GCV, GCV-TP levels were 2.6-fold higher compared to GCV treatment alone. [H3]GCV incorporation into DNA was also elevated more than 2-fold in pretreated cells compared to simultaneous addition. In addition, compared to GCV treatment alone, sequential addition of 5-FC and GCV resulted in an additional 60% growth inhibition, whereas only 15% inhibition was observed when both drugs were given together. We measured the effect of 5-FC pre-incubation on endogenous deoxynucleotide concentrations by incubating DU145 cells transduced with the CD/HSV-TK adenovirus with 200 μg/ml 5-FC over a 24 hr period. As expected, cellular dTTP levels were gradually depleted over the 24 hr incubation (from 82 to 11 pmol/106 cells). However, the level of dGTP also decreased (from 9 to 1 pmol/106 cells) concurrently with the dTTP pool decrease over this time period. This reduction of dTTP and dGTP concentrations was not observed when CD/HSV-TK-expressing DU145 cells were treated over 24 hr with both 5-FC and GCV simultaneously. The results from these experiments allowed us to propose and test a novel hypothesis for the synergistic interaction between CD/5-FC and HSV-TK/GCV treatments. We suggest that the CD/5-FC-mediated reduction of dTTP concentrations results in concurrent decrease of dGTP levels due to allosteric regulation of ribonucleotide reductase. Since dGTP is the endogenous competitor of GCV-TP, a depleted dGTP pool at the time of GCV addition can result in increased GCV incorporation into DNA and cell kill. In fact, including increasing amounts of deoxyguanosine during the 5-FC pre-incubation reverses the depletion of endogenous dGTP, reduces the amount of GCV incorporated into DNA and negates the GCV-mediated cytotoxicity associated with 5-FC pre-incubation in DU145 cells expressing CD and HSV-TK. Understanding this mechanistic interaction between these two enzyme/prodrug systems may help recognize better strategies for creating more efficacious clinical protocols.
FDA Official Appointed
Authors: Tracy Hampton
Journal: JAMA (2006)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 3660: Chronic alcohol consumption inhibits iNKT cell activation-induced antitumor response in B16BL6 melanoma-bearing mice
Authors: Hui Zhang, Faya Zhang, ZhaoHui Zhu, Gary G. Meadows
Journal: Cancer Research (2014)

Abstract Alcohol consumption increases the incidence of many types of cancer including melanoma. It is not well understand how chronic alcohol consumption affects tumor progression and survival. We previously found that chronic alcohol consumption activates CD8+ T cells in the non-tumor injected mice and at the early stage after tumor inoculation; however, it accelerates CD8+T cell dysfunction during further tumor growth. The underlying mechanism regarding modulation of CD8+ T cells by alcohol and how the functional changes in the immune response affect tumor growth and survival remain to be elucidated. Herein, we investigated how alcohol modulates iNKT cells in melanoma-bearing mice and how different immune treatment regimens affect tumor growth and survival. We found that alcohol increases iNKT cells in non-tumor injected mice, and that activation of these iNKT cells induces a Th1 response. With tumor progression, alcohol reverses iNKT cell cytokine profile to aTh2-dominant one. Chronic alcohol consumption inhibits melanoma growth, but this is does associated with increased survival. Immunization with a melanoma cell lysate significantly inhibits tumor growth and increases survival compared to the non-immunized alcohol-consuming, tumor-bearing mice. Immunization in the water-drinking control mice did not significantly increase survival compared to respective non-immunized mice. Immunization coupled with iNKT cell activation with alpha GalCer significantly increases the survival of water-drinking melanoma-bearing mice. However, the survival of mice consuming alcohol was significantly decreased compared to their water-drinking counterparts. Collectively, chronic alcohol consumption interacting with melanoma alters the iNKT cell cytokine profile from Th1-dominant to Th2- dominant, which in turn contributes to inhibition of antitumor immunity and decreases survival of melanoma-bearing mice. Supported by NIH grants R01AA07293 and K05AA017149 and funds provided for medical and biological research by the State of Washington Initiative Measure No. 171. Citation Format: Hui Zhang, Faya Zhang, ZhaoHui Zhu, Gary G. Meadows. Chronic alcohol consumption inhibits iNKT cell activation-induced antitumor response in B16BL6 melanoma-bearing mice. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3660. doi:10.1158/1538-7445.AM2014-3660
A model of homelessness among male veterans of the Vietnam War generation
Authors: R Rosenheck, A Fontana
Journal: American Journal of Psychiatry (1994)

This study explored a multifactorial model of vulnerability to homelessness among male veterans of the Vietnam war generation.Data from 1,460 male veterans who participated in the National Vietnam Veterans Readjustment Study were used to evaluate hypotheses about the causes of homelessness grouped into four sets of sequential variables: 1) premilitary risk factors, 2) war related and non-war-related traumatic experiences, 3) lack of social support at the time of discharge from military service, and 4) postmilitary psychiatric disorder and social dysfunction. Structural equation modeling was used to explore the posited model of risk factors for homelessness.Postmilitary social isolation, psychiatric disorder, and substance abuse had the strongest direct effects on homelessness, although substantial indirect effects from stressors related to being in the war zone and from premilitary conduct disorder were observed. Several premilitary factors--year of birth, childhood physical or sexual abuse, other childhood traumas, and placement in foster care during childhood--also had direct effects on homelessness.Individual vulnerability to homelessness is most likely due to a multiplicity of psychiatric and nonpsychiatric factors, with independent influences emerging at each of four discrete time periods. In view of this complex pattern of influences, prevention efforts directed at individuals must address a very broad range of adjustment problems.
Abstract 599: The Effect of Insulin-induced Hypoglycaemia on Myocardial Blood Flow in Patients With Type 1 Diabetes Mellitus
Authors: Omar Rana, Soha Zouwail, David V Coppini, Roxy Senior, Joe Begley, David Kerr, Christopher D Byrne, Kim Greaves
Journal: Circulation (2009)

Background and Purpose: Hypoglycaemia is a frequent complication of insulin therapy in patients with diabetes mellitus (DM) and is associated with increased cardiovascular mortality. This study ass...