tbuckley/cabot-search huggingface dataset:
Abstract 150: Role of reactive oxygen species in doxorubicin-induced apoptosis of myeloid-derived suppressor cells
Authors: Darya Alizadeh, Emmanuel Katsanis, Nicolas Larmonier
Journal: Cancer Research (2014)

Abstract The expansion of myeloid-derived suppressor cells (MDSC) in the blood, secondary lymphoid organs and tumor beds in animal cancer models and in patients with many types of cancers participates to the immunosuppressive environment associated with the development of tumors. MDSC frequency correlates with the disease stage and prognosis. MDSC can not only impair innate and adaptive antitumor immune responses, but these cell also contribute to enhance tumor angiogenesis and metastatic potential. The therapeutic targeting of MDSC (depletion,functional inactivation or differentiation into pro-inflammatory cells) promotes the efficacy of immunotherapeutic interventions. Conventional anticancer agents such as docetaxel, gemcitabine or sunitinib have been used to eliminate tumor-induced MDSC. We have observed that the anthracycline doxorubicin can deplete MDSC that expanded in the murine mammary tumor models 4T1 and EMT6 and in the EL4 thymoma model. Doxorubicin-mediated elimination of MDSC is associated with increased T and NK cell activity and the combination of this drug with adoptive T lymphocyte transfer impairs tumor development and metastatic spreading. We here present results indicating that doxorubicin-mediated apoptosis of MDSC may depend on an increased production of reactive oxygen species (ROS) by these cells. MDSC from gp91-/- mice (lacking the gp91phox glycosylated subunit of the NADPH oxidase flavocytochrome b558, responsible for the production of superoxide ion O2·-) were less sensitive to doxorubicin-induced cell death than their wild-type counterparts. Consistently, the effects of doxorubicin on MDSC were partially impaired in tumor-bearing gp91-/- animals compared to wild-type mice. Interestingly the level of ROS in CD4+ or CD8+ T and NK cells were low and unchanged by doxorubicin administration. These observations may partly explain the selective effects of this chemotherapeutic agent on immunosuppressive MDSC while effector lymphocytes are spared and the subsequent increased in the effector to suppressor cell ratios, resulting in a mitigated immunosuppressive tumor environment. Citation Format: Darya Alizadeh, Emmanuel Katsanis, Nicolas Larmonier. Role of reactive oxygen species in doxorubicin-induced apoptosis of myeloid-derived suppressor cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 150. doi:10.1158/1538-7445.AM2014-150
Abstract 1672: Tumor educated B cells acquire immune suppressive function and promote tumor growth
Authors: Yu Zhang, Richard Morgan, Seung-uon Shin, Hyun-Mi Cho, Ahmed Albayati, Augustin Pimentel, Joseph D. Rosenblatt
Journal: Cancer Research (2014)

Abstract B lymphocytes may play a role in inhibiting the immune response against certain tumors, but the underlying mechanisms by which B cells facilitate tumor growth are poorly understood. Murine EMT-6 mammary tumors grow readily in wild type mice (WT) but are rejected or grow very poorly in B-cell deficient μ-/- BALB/c mice (BCDM). Relative to BCDM, B cell reconstituted BCDM demonstrate enhanced tumor growth, marked expansion of Tregs in response to EMT-6 implantation, increased infiltration of both B cells and Tregs into tumor, and a markedly reduced cytolytic T cell response. Markedly decreased levels of CD49b+ NK cell and CD8+ T cell infiltration into the tumor bed were seen in WT or in B cell reconstituted BCDM, compared to levels of infiltration seen in BCDM. Adoptive transfer of either IL-10-/- B cells or MHC-II-/- B cells into BCDM also promoted tumor growth and expansion of Treg and suppressed cytotoxic CD8+ T cell anti-tumor activity and IFN-γ production in vivo. We hypothesized that tumor infiltrating B cells promote Treg expansion but also may directly suppress T cell activation and proliferation due to acquired regulatory function. In vitro co-culture of EMT-6 tumor cells with naïve B cells generated B cells of a distinct phenotype (PD-L1hiCD86hiIAdhiCD62LhiLAP+CD44lo but CD25-CD69-CD80-). EMT-6 co-cultured B cells markedly suppressed naïve CD4 T cell proliferation. Flow cytometric analysis of tumor infiltrating B cells (TIL-B) revealed that at day 21 post tumor implantation, TIL-B cells are LAPhiCD62LhiCD86hiIAdhiPD-L1hiCD44+CD25-. LAP/TGF-β expression on TIL-B cells was gradually upregulated as tumor burden increased, from 2∼3% on day 8 to ∼50% of infiltrating B cells by day 21. In contrast, splenic B cells at day 21 did not express LAP/TGF-β. TIL-B cells also markedly suppressed CD4+ T cell proliferation in-vitro when compared to splenic B cells. Our studies indicate that the T cell anti-tumor response is enhanced in the absence of B cells, due to acquisition of a suppressive immmunoregulatory phenotype by migrating B cells following tumor infiltration. We are currently looking for evidence of human correlates of B regulatory activity observed in murine models. Citation Format: Yu Zhang, Richard Morgan, Seung-uon Shin, Hyun-Mi Cho, Ahmed Albayati, Augustin Pimentel, Joseph D. Rosenblatt. Tumor educated B cells acquire immune suppressive function and promote tumor growth. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1672. doi:10.1158/1538-7445.AM2014-1672
Abstract 11088: Electrophysiologic and Anatomical Substrates of Atypical Atrial Flutter: Importance of Electrically Abnormal, Low-Amplitude Lesion in the Circuit
Authors: Taihei Itoh, Shingo Sasaki, Masaomi Kimura, Shingen Owada, Horiuchi Daisuke, Kenichi Sasaki, Ken Okumura
Journal: Circulation (2010)

Atrial flutter (AFL) is due to reentry around the tricuspid annulus (typical AFL) or other anatomical obstacle and/or electrically abnormal lesion (atypical AFL). Atypical AFL is considered to have a smaller circuit than typical AFL, but its electrophysiologic characteristics are not fully understood. In 26 patients with typical (n=10) and atypical right atrial AFL with (incisional reentry, n=12) or without surgical scar (non-incisional reentry, n=4), CARTO map during AFL was created by electroanatomical data from 224±88 right atrial sites. Low amplitude electrogram (LAE) was defined as the electrogram <0.5 mV and scar as the electrogram <0.1 mV. In all patients, the reentry circuit confined to the right atrium was identified by CARTO map and by entrainment mapping. AFL cycle lengths (CL) of typical AFL and atypical non-incisional and incisional AFL were 219±25, 282±75, and 290±61 msec, respectively (p<0.05 among 3 groups by Kruskal-Wallis), lengths of reentry path 163±18, 162±42, and 124±46 mm, respectiv...
Abstract 5517: A Novel Tissue Engineered Skeletal Muscle Screening Platform to Assess the Myotoxic Potential of Statins
Authors: Malte Tiburcy, Peter Christalla, Thomas Eschenhagen, Wolfram H Zimmermann
Journal: Circulation (2008)

Our study had two objectives: to develop a novel tissue engineered skeletal muscle drug screening platform and to assess myotoxicity of various statins and identify underlying mechanisms. Methods: Engineered skeletal muscle tissue (SMT) was generated from adult rat skeletal myoblasts, rat fibroblasts (1.25x106 cells/SMT), matrigel, and collagen. Reconstitution-mixtures (450 μl) were poured into circular moulds yielding ring-shaped constructs after 5 days. SMTs were cultured for additional 10 days on stretch devices and treated with increasing concentrations of statins or vehicle for the last 5 culture days. Force of contraction (FOC) was measured and SMTs subjected to either immune fluorescence (IF), HE-staining or Western blotting (WB). Results: SMTs displayed differentiated muscle bundles (actin-IF and HE) and elicited tetanic contractions with maximal force at 60 Hz (1.3±0.2 mN; n=5). Carbachol (1 μM) induced a reversible block of muscle contraction which was antagonized by pancuronium (10 μM) indicating the presence and functionality of nicotinergic acetylcholine receptors. Statin treatment caused a concentration-dependent decrease in FOC in the following order of potency (EC50): cerivastatin (10 nM), simvastatin (100 nM), atorvastatin (350 nM), pravastatin (1860 nM; n=6–8/group and concentration). Contractile failure was paralleled by sarcomere breakdown and apoptosis (IF and WB: reduced actin, increased caspase 3). To assess the mechanism of statin myotoxicity, we investigated the protective effects of mevalonic acid (MEV, 100 μM), farnesyl-pyrophosphate (F-PP, 10 μM), squalene (S, 10 μM), and geranyl-geranyl-pyrophophate (G-PP, 10 μM) in the presence of maximally toxic cerivastatin concentrations (1 μM; complete contractile failure). MEV prevented cerivastatin toxicity completely (FOC: 102.3±16% of vehicle; n=7). GG-PP partially prevented cerivastatin-induced contractile failure (FOC: 53.3±14% of vehicle; n=4). S and F-PP had no protective effect (n=4/group). Conclusion: SMT can be used to identify and compare statin myotoxicity in vitro and may be useful to dissect its underlying mechanisms. In addition, our data suggests that statin myotoxicity is at least in part independent of cholesterol depletion.
Openness in Biomedical Research Collides With Heightened Security Concerns
Authors: Brian Vastag
Journal: JAMA (2003)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Encounters between Patients and Doctors. An Anthology.
Authors: John D. Stoeckle
Journal: Annals of Internal Medicine (1987)

This book of readings offers compelling evidence that even in this age of advanced medical technology and large-scale bureaucracy, the essence of diagnosis, therapy, and management of illness continues to reside in the doctor-patient relationship. It brings together 17 articles published from 1927 to 1978 dealing with the sociological and cultural aspects of clinical and bedside medicine.The articles are grouped into five parts. These cover the ground rules for the doctor-patient relationship, the dynamics of the interchange, the nature of the communication, barriers to communication, case studies, and visions of what the ideal relationship might be. The editor has added an extensive introduction that situates the articles and reviews in the current state of research in the field.Authors included are Emily M. Ahern, Arlene K. Daniels, Milton S. Davis, Sherman Eisenthal, Joan P. Emerson, Vida Francis, Arlene Frank, Eliot Freidson, L. J. Henderson, Marc H. Hollender, Arthur Kleinman, Barbara M. Korsch, Aaron Lazare, John B. McKinlay, Marie J. Morris, Talcott Parsons, Francis W Peabody, Thomas J. Scheff, Thomas S. Szasz, Howard Waitzkin, Harry A. Wilmer, and Irving Kenneth Zola.John D. Stoeckle, M.D., is Professor of Medicine at Harvard Medical School and Director of the Primary Care Program at Massachusetts General Hospital. He is coauthor, with George Abbott White, of Plain Pictures of Plain Doctoring: Vernacular Expression in New Deal Medicine and Photography (MIT Press 1985).
50. AAV-Mediated Expression and Secretion of ADNF-9 Protects Substantia Nigra Neurons from 6-OHDA Damage
Authors: Shin-ichi Muramatsu, Fumiko Ono, Yuko Nara, Mika Kodera, Naomi Takino, Junko Tsuchida, Katsuyoshi Kawasaki, Kunihiko Ikeguchi, Kenichi Fujimoto, Hideo Tsukada, Keiji Terao, Imaharu Nakano, Keiya Ozawa
Journal: Molecular Therapy (2004)
Abstract 12525: Persistent No Reflow Phenomenon After Percutaneous Coronary Intervention in Patients With Acute Myocardial Infarction Was Associated With a Poor In-Hospital Outcome and Increased Long-Term Mortality, Mainly Driven by Increased Cardiac Mortality
Authors: Min Chul Kim, Hae Chang Jeong, Keun Ho Park, Doo Sun Sim, Young Joon Hong, Ju Han Kim, Myung Ho Jeong, Jeong Gwan Cho, Jong Chun Park, Youngkeun Ahn
Journal: Circulation (2014)

Introduction: There is limited information on the transient or persistent no reflow phenomena in patients with acute myocardial infarction (AMI) undergoing percutaneous coronary intervention (PCI). Hypothesis: We aimed to evaluate the long-term clinical outcomes of transient of persistent no reflow phenomenon following PCI in patients with AMI. Methods: The study analyzed 4329 patients with AMI who underwent PCI using coronary stents in the COnvergent REgistry of cAtholic and chonnAm university for Acute MI (COREA-AMI) from 2004 to 2009 (2668 ST-elevation and 1661 non-ST-elevation MI patients): 4071 patients without any no reflow during PCI, 213 patients with transient no reflow (final thrombolysis in myocardial infarction [TIMI] flow grade 3 after adequate management of no reflow), and 45 patients with persistent no reflow (final TIMI flow grade ≤ 2 despite of management for no reflow). The primary endpoint was all-cause mortality during 3-year follow-up. We also analyzed the incidence of cardiac death, ...
The role of the combined assessment of the bronchoalveolar lavage (BAL) and radiological profile in the diagnosis of chronic eosinophilic pneumonia (CEP)
Authors: Stylianos Michaelides, Konstantina Tsakanika, Adamantia Englezopoulou, Constantinos Paraskevopoulos, Angeliki Petoumenou, Eftychia Kourtelelessi, George Goulas
Journal: European Respiratory Journal (2013)

This study aimed to assess the role of the of cytomorphological findings of BAL in combination with patients’ radiological profile in establishment CEP diagnosis. This condition being a relatively uncommon condition is characterized by dense focal alveolar infiltrates and symptoms of dyspnea, cough, malaise and peripheral eosinophilia. We studied the BAL sediments and radiological features of 11 patients (6 male & 5 female) aged 43±5.7 yrs. (mean±SD)) who were hospitalized during the last 5 years. They all presented with compatible symptoms and diffuse bilateral alveolar shadows. Peripheral blood eosinophils, C-reactive protein and erythrocyte sedimentation rate were elevated in 10 of the 11 patients (91%).All patients had a chest CT-scan and underwent fiberoptic bronchoscopy with BAL. The BAL cell profile showed a variable pattern with a pronounced elevation of eosinophil count (>25%) which was observed in all patients. CT-scans revealed bilateral infiltrates with a predilection for upper lung zones. Lower zone predilection was identified in only one patient. The characteristic “photographic negative of pulmonary edema” distribution was observed in 5 (45.5%) of the patients. Our patients were treated with corticosteroids and had a prompt and dramatic clinical snd radiological improvement. It is concluded that in the appropriate clinical context, combination of BAL findings with the radiological characteristics can practically confirm the diagnosis of CEP rendering more interventional procedures (Video-assisted thoracoscopic or open lung biopsy) rarely necessary for accurate diagnosis and leading to successful treatment.
Abstract 63: Early Epinephrine Administration Within 15 Minutes After EMS Call Led to Favorable Neurological Outcome
Authors: Tanaka Hidhearu, Hiroshi Takyu, Takashi Nakagawa, Shirakawa Toru, Soh Gotoh, Eiichi Hasegawa, Tomayo Kinoshi, Hiroyuki Takahashi, Etsuko Sone, Syuji Shimazaki
Journal: Circulation (2014)

Introduction:  Effectiveness of epinephrine administration on OHCA patients has been extensively explored, but there is no definitive evidence to improving improve favorable neurological outcome.   Hypothesis:  We hypothesized if we have successfully given epinephrine early stage of cardiac arrest, it may improve the neurological outcome.   Methods:  A total 925288 OHCA patients registered between January 2005 and December 2012 on Japan’s nationwide registry database. Among these, 100338 patients aged 15-89 with witnessed cardiac arrest due to cardiac cause origin were eligible for our analyses. We excluded all of SGA device applied and Tracheal Intubated case to neglect for their effects, outer timeline data, and ROSC before epinephrine administration. In fact, 6099 patients received prehospital epinephrine administration with Bag Valve Mask (as BVM) ventilation by Emergency lifesaving technician (as Epi group;12.5%) divided into following time span (as t), 9≦t   Results:  In patients with the early Epi- group had a significantly higher CPC-1/2 rate compared with the non-Epi group (12.2% vs.8.0% unadjusted odds ratio 1.59, CI 1.23-2.05) and Also, Early Epi groups indicates significantly higher CPC-1/2 rate than that of the Late-Epi groups (12.1% vs.3.4% unadjusted odds ratio3.98, CI 2.98-5.33).   Conclusions:  We conclude that early epinephrine administration within 15 minute after EMS call, Emergency lifesaving technician could have chance to improves the long term neurological outcome in patients with witness cardiogenic OHCA. Epinephrine for OHCA should be given within 15 minutes after EMS call. In order to carry it out, it prefer to local EMS protocol and re-training curriculum change. Further investigation of effectiveness of early epinephrine administration must be warrant.
Long-term follow-up of remission duration, mortality, and second malignancies in hairy cell leukemia patients treated with pentostatin.
Authors: Ian W. Flinn, Kenneth J. Kopecky, M. Kathryn Foucar, David Head, John M. Bennett, Robert Hutchison, William Corbett, Peter Cassileth, Thomas Habermann, Harvey Golomb, Kanti Rai, Elizabeth Eisenhauer, Frederick Appelbaum, Bruce Cheson, Michael R. Grever
Journal: Blood (2000)
A Directly Spliced Exon 10–Containing CD44 Variant Promotes the Metastasis and Homotypic Aggregation of Aggressive Non-Hodgkin's Lymphoma
Authors: Yoshihiro Yakushijin, Joshua Steckel, Samir Kharbanda, Robert Hasserjian, Donna Neuberg, Wei-meng Jiang, Ian Anderson, Margaret A. Shipp
Journal: Blood (1998)
THE MECHANISMS OF HEALING IN COLLAPSE THERAPY
Authors: Max Pinner
Journal: Annals of Internal Medicine (1935)

Article1 November 1935THE MECHANISMS OF HEALING IN COLLAPSE THERAPYMAX PINNER, M.D., F.A.C.P.MAX PINNER, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-9-5-501 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe results of collapse therapy, despite their spectacular improvement during the last decade, are often inconstant, unpredictable and disappointing. As culled from the literature the reasons for success or failure are manifold and often contradictory. On the other hand, a large number of clinical, pathological and experimental studies provides a sufficient basis for a discussion of the factors that play a beneficial or disturbing rôle in collapse therapy. While many single phases have been studied carefully, I failed to find in the literature a comprehensive discussion of the problem as a whole. A clear conception of the physiology of pulmonary... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: *Read at the Philadelphia meeting of the American College of Physicians, April 30, 1935.From the Laboratories of the Desert Sanatorium and Institute of Research, Tucson, Arizona. Nextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byHEMIDIAPHRAGMATIC PARALYSIS IN THE TREATMENT OF PULMONARY TUBERCULOSISArtificial pneumothorax 1 November 1935Volume 9, Issue 5Page: 501-515KeywordsRespiratory physiology ePublished: 1 December 2008 Issue Published: 1 November 1935 PDF downloadLoading ...
Abstract 15309: Ghrelin Protects Heart Against Ischemia-Induced Arrhythmias by Preserving Connexin43 Protein
Authors: Takeshi Soeki, Toshiyuki Niki, Sachiko Bando, Sahika Hisaoka, Hidekazu Takeuchi, Kenya kusunose, Koji Yamaguchi, Yoichiro Hirata, Noriko Tomita, Yoshio Taketani, Takashi Iwase, Hirotsugu Yamada, Tetsuzo Wakatsuki, Masashi Akaike, Kenji Kangawa, Masataka Sata
Journal: Circulation (2010)

Background: Vagal nerve stimulation has been postulated to contribute to the antifibrillatory effect. Recently we have shown that ghrelin modulates cardiac autonomic activity and attenuates early l...
Children’s Antiretroviral Combo
Authors: Rebecca Voelker
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal