tbuckley/cabot-search huggingface dataset:
Americans' Views on Children's Health
Authors: R J Blendon, J T Young, M C McCormick, M Kropf, J Blair
Journal: JAMA (1998)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Different in vivo reactivity profile in health care workers and patients with spina bifida to internal and external latex glove surface-derived allergen extracts
Authors: C.M. Peixinho, P. Tavares-Ratado, M.F. Gabriel, A.M. Romeira, C. Lozoya-Ibanez, L. Taborda-Barata, C.T. Tomaz
Journal: British Journal of Dermatology (2011)

Allergy to natural rubber latex is a well-recognized health problem, especially among health care workers and patients with spina bifida. Despite latex sensitization being acquired in health institutions in both health care workers and patients with spina bifida, differences in allergen sensitization profiles have been described between these two risk groups.To investigate the in vivo reactivity of health care workers and patients with spina bifida to extracts of internal and external surfaces of latex gloves and also to specific extracts enriched in major allergens for these risk groups.Gloves from different manufacturers were used for protein extraction, and salt precipitation and hydrophobic interaction chromatography (HIC) were applied to obtain the enriched latex extracts. The major latex allergens were quantified by an enzyme immunoassay. The extracts obtained were tested in 14 volunteers using skin prick tests (SPT).Latex glove extracts enriched in the hydrophobic allergens that are most often seen in patients with spina bifida were obtained by selective precipitation, whereas HIC produced extracts enriched in the hydrophilic allergens commonly found in health care workers. The health care workers had positive SPTs to glove extracts from internal surfaces and to the hydrophilic allergen-enriched extracts. By contrast, patients with spina bifida had larger skin reactions both to external glove extracts and to the extracts enriched with the hydrophobic major allergens for this risk group. Despite the protein concentration of these extracts being less than half the concentration of the commercial extract, the weal-and-flare reactions were of similar magnitude.Using novel latex extracts, our study showed a different in vivo reactivity pattern in health care workers and in patients with spina bifida to extracts of the internal and external surfaces of gloves, which suggests that sensitization may occur by different routes of exposure, and that this influences the allergen reactivity profiles of these risk groups.
Peripheral T cell receptor diversity is associated with clinical outcomes following ipilimumab treatment in metastatic melanoma
Authors: Michael A. Postow, Manuarii Manuel, Phillip Wong, Jianda Yuan, Zhiwan Dong, Cailian Liu, Solène Perez, Isabelle Tanneau, Marlène Noel, Anaïs Courtier, Nicolas Pasqual, Jedd D. Wolchok
Journal: Journal for ImmunoTherapy of Cancer (2015)

Ipilimumab improves overall survival in a subset of patients with metastatic melanoma. Peripheral blood T cell receptor (TCR) repertoire diversity has been associated with favorable outcomes in patients with cancer, but its relevance as a biomarker for ipilimumab outcomes remains unknown.In this pilot study, we analyzed the pre-treatment peripheral blood TCR repertoire in 12 patients with metastatic melanoma who received ipilimumab at 3 mg/kg (clinical benefit, n = 4; no clinical benefit, n = 8). TCR diversity was evaluated using a polymerase chain reaction assay which measures TCR combinatorial diversity between V and J genes from genomic DNA. TCR repertoire diversity was studied through richness (observed V-J rearrangements) and evenness (similarity between the frequencies of specific V-J rearrangements). The Wilcoxon rank sum test was used to compare patients with clinical benefit and those without. Association with benefit in a dichotomized analysis was assessed through a Fisher's exact test. Overall survival was studied through log-rank analysis. There was a significant difference in richness (p = 0.033) and evenness (p = 0.028) between patients with and without clinical benefit. Dichotomized analysis showed that none of the patients with low richness (n = 0/5, p = 0.081) nor low evenness (n = 0/7, p = 0.01) achieved clinical benefit. There were no significant differences in overall survival.In this small group of patients, baseline TCR diversity in the peripheral blood was associated with clinical outcomes. Further investigation is ongoing in larger cohorts of patients to explore these preliminary findings and determine whether TCR diversity can be used as a predictive biomarker in cancer immunotherapy.
ApoA-1 Milano and Regression of Atherosclerosis
Authors: Hyuntae Kim, Elaine L. Jacobson, Myron K. Jacobson, Andras G. Lacko
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 18584: Alpha-2 Agonist Attenuates Ischemic Injury in Isolated Spinal Cord Neurons
Authors: Kirsten A Freeman, Ferenc Puskas, Marshall T Bell, Joshua M Mares, Michael J Weyant, Joseph C Cleveland, David A Fullerton, Xianzhong Meng, Paco Herson, Thomas B Reece
Journal: Circulation (2013)

Background: Paraplegia secondary to spinal cord ischemia-reperfusion injury remains a devastating complication of thoracoabdominal aortic surgery. The complex interactions between the injured neurons and activated leukocytes have made investigation of neuron specific injury elusive. We hypothesize that isolated spinal motor neuron cell cultures subjected to oxygen-glucose deprivation (OGD) would simulate ischemia-reperfusion (IR) injury seen in aortic surgery, which could be attenuated by specific alpha-2a agonism, using dexmedetomidine, in an Akt dependent fashion. Methods and Results: Spinal cords from perinatal mice were harvested and neurons cultured in vitro for 7-10 days. Cells were pretreated with 1μM dexmedetomidine (Dex) and subjected to OGD in a humidified hypoxic chamber. Indexed to non-ischemic controls, pretreatment with Dex significantly preserves neuronal viability after one hour of OGD with 88% viability compared to untreated OGD with 59% viability (p&lt;0.05) . TUNEL staining demonstrated pretreatment with Dex significantly decreased the apoptotic cells compared to untreated OGD cells by over 50% (p&lt;0.05). OGD increased IL-6 production nearly three-fold compared to non-ischemic control (p&lt;0.05) with Dex reducing this production by half (p&lt;0.05). Mechanistically, Dex treatment significantly increased phosphorylated Akt (p&lt;0.05), but the protective effects of Dex seen by MTT assay were eliminated using an alpha-2a antagonist or an Akt inhibitor (p&lt;0.05), with viability nearly half of non-ischemic control. Conclusion: Using a novel spinal cord neuron cell culture, oxygen-glucose deprivation mimics neuronal metabolic derangement responsible for paraplegia following aortic surgery. Dexmedetomidine preserves neuronal viability and decreases apoptosis in an Akt dependent fashion. Dexmedetomidine demonstrates clinical promise for reducing the risk of paraplegia following high risk aortic surgery.
Abstract 13520: Sex Differences in Clinical Characteristics and Outcomes of Patients with Heart Failure with Preserved Ejection Fraction -A Report from the CHART-2 Study
Authors: Kanako Tsuji, Yasuhiko Sakata, Masanobu Miura, Satoshi Miyata, Kotaro Nochioka, Soichiro Tadaki, Ryoichi Ushigome, Takeshi Yamauchi, Kenjiro Sato, Takeo Onose, Ruri Abe, Jun Takahashi, Hiroaki Shimokawa
Journal: Circulation (2014)

Background: Several studies have reported that the prognosis of patients with heart failure with preserved ejection fraction (HFpEF) was similar to that of patients with reduced ejection fraction. However, sex differences in clinical characteristics and mortality of HFpEF patients remains to be fully elucidated. Methods: Among the 10,219 patients registered in our Chronic Heart Failure Analysis and Registry in the Tohoku District-2 (CHART-2) Study, we examined sex differences in clinical characteristics, treatment and long-term outcome of 3,124 patients with HFpEF in stage C/D (EF≥50%, mean age 69.4 years, 34.7% female). We constructed the Kaplan-Meier curves and Cox hazard models for all-cause death to evaluate sex difference in mortality. Results: Female patients, as compared with male patients, were characterized by higher age (71.6 vs. 68.3 years, P&lt;0.001), higher prevalence of NYHA class III/IV (14.1 vs. 7.0%, P&lt;0.001), higher left ventricular ejection fraction (67.2 vs. 64.5%, P&lt;0.001), and higher brain natriuretic peptide levels (106 vs. 72.9pg/ml, P&lt;0.001). Furthermore, less female patients received beta-blockers (37.8 vs. 43.9%, P=0.001) and renin-angiotensin system inhibitors (64.7 vs. 71.8%, P&lt;0.001) compared with males. During the median 3.2-year follow-up, 392 patients (12.5%) died. Although Kaplan-Meier curves showed that the crude mortality was comparable between males and females (hazard ratio (HR) 1.18, P=0.11), the adjusted mortality risk was significantly lower in females (HR 0.72, P=0.009) (Figure). Female patients died due to cardiovascular causes more frequently than males (53.7 vs. 39.2%, P&lt;0.01). Conclusions: Although female HFpEF patients had comparable all-cause mortality with males, they had severer HF and higher cardiovascular mortality than males.
FRUCTOSURIA: REPORT OF A CASE
Authors: Alfred R. Lenzner
Journal: Annals of Internal Medicine (1956)

Case Reports1 October 1956FRUCTOSURIA: REPORT OF A CASEALFRED R. LENZNERALFRED R. LENZNERAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-45-4-702 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptAll melituria is not glucosuria. This perfectly sound statement seems acceptable to all. But the fact is easily forgotten. Melituria associated with glycemia exceeding the accepted range of normal is naturally assumed to be true glucosuria. And in time one's thinking may become so tracked clinically as to assume that melituria exhibited simultaneously with a normal glucose tolerance is likewise simple glucosuria, perhaps of the "low threshold" type. Such was the impression after four loading tests in the instance cited below. A family history of diabetes seemed to confirm this deduction.CASE REPORTA 20 year old soldier entered the...Bibliography1. Stoehr R: Ueber eine einfache Methode zur Bestimmung der Fructose im Blut, Klin. Wchnschr. 13: 179-180, 1934 (Abstract). CrossrefGoogle Scholar2. Steinitzvon Riesen HI: Mikrobestimmung der Fructose im Blut, Biochem. Ztschr. 252: 201, 1932. Google Scholar3. Lasker M: Essential fructosuria, Human Biol. 13: 51-63, 1941. Google Scholar4. MillerDruckerOwensCrainWoodward MWRJEJWH: Metabolism of intravenous fructose and glucose in normal and diabetic subjects, J. Clin. Investigation 31: 115-125, 1952. CrossrefMedlineGoogle Scholar5. CoriOchoaSleinCori GTSMWCF: The metabolism of fructose in liver. Isolation of fructose-1-phosphate and inorganic pyrophosphate, Biochim. & Biophys. Acta 7: 304-317, 1951. CrossrefMedlineGoogle Scholar6. SleinCoriCori MWGTCF: A comparative study of hexokinase from yeast and animal tissues, J. Biol. Chem. 186: 763-780, 1950. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: *Received for publication December 21, 1955.From the U. S. Army Hospital, Wurzburg, Germany, March, 1954.Requests for reprints should be addressed to Alfred R. Lenzner, M.D., 685 Delaware Avenue, Buffalo 9, N. Y. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byA mathematical analysis of adaptations to the metabolic fate of fructose in essential fructosuria subjectsMellituria, a paper chromatographic studyEssential Benign FructosuriaAngeborene heredit�re Fructose-IntoleranzDie nichtdiabetischen Melliturien 1 October 1956Volume 45, Issue 4Page: 702-706KeywordsFructosesGlucose toleranceHydrochloric acidIngestion Issue Published: 1 October 1956 PDF downloadLoading ...
Primary Anaplastic Large-Cell Lymphoma in Adults: Clinical Presentation, Immunophenotype, and Outcome
Authors: Hervé Tilly, Philippe Gaulard, Eric Lepage, Charles Dumontet, Jacques Diebold, Isabelle Plantier, Françoise Berger, Michel Symann, Tony Petrella, Pierre Lederlin, Josette Brière
Journal: Blood (1997)
Molecular and prognostic correlates of cytogenetic abnormalities in chronic myelomonocytic leukemia: a <scp>M</scp>ayo <scp>C</scp>linic‐<scp>F</scp>rench <scp>C</scp>onsortium <scp>S</scp>tudy
Authors: Emnet A. Wassie, Raphael Itzykson, Terra L. Lasho, Olivier Kosmider, Christy M. Finke, Curtis A. Hanson, Rhett P. Ketterling, Eric Solary, Ayalew Tefferi, Mrinal M. Patnaik
Journal: American Journal of Hematology (2014)

Four hundred and nine patients with chronic myelomonocytic leukemia (CMML) were included in the international collaborative study (268 (66%) and 141 (34%) from Mayo clinic and French consortium respectively). Thirty percent displayed an abnormal karyotype, including; 72% sole, 16% two, and 11% complex abnormalities. The most common abnormalities included; +8 (23%), -Y (20%), -7/7q-(14%), 20q- (8%), +21 (8%), and der(3q) (8%). Patients with an abnormal karyotype were more likely to be elderly (P = 0.03), be anemic (P = 0.0009), have leukocytosis (P = 0.02) with neutrophilia (P = 0.03), demonstrate increased circulating immature myeloid cells (P = 0.0003), peripheral blood blasts (P < 0.0001), and bone marrow blasts (P < 0.0001). ASXL1 (P = 0.04) and SF3B1 (P = 0.03) mutations clustered with an abnormal karyotype, whereas SRSF2 (P = 0.02) mutations occurred more commonly with a normal karyotype. A step-wise survival analysis resulted in three distinct cytogenetic risk categories: high (complex and monosomal karyotypes), intermediate (all abnormalities not in the high or low risk groups) and low [normal, sole -Y and sole der (3q)] with median survivals of 3 [hazard ratio (HR) = 8.1, 95% confidence interval (CI) = 4.6-14.2], 20 (HR = 1.7, 95% CI = 1.2-2.3) and 41 months, respectively. In multivariable analysis, this particular cytogenetic risk stratification remained significant in the context of the Molecular Mayo Model (P < 0.0001), MD Anderson prognostic model (P < 0.0001), the GFM CMML model (P < 0.0001) and was effective in predicting leukemic transformation (P = 0.004).
New views on RPE65 deficiency: the rod system is the source of vision in a mouse model of Leber congenital amaurosis
Authors: Mathias W. Seeliger, Christian Grimm, Fredrik Ståhlberg, Christoph Friedburg, Gesine Jaissle, Eberhart Zrenner, Hao Guo, Charlotte E. Remé, Peter Humphries, Franz Hofmann, Martin Biel, Robert N. Fariss, T. Michael Redmond, Andreas Wenzel
Journal: Nature Genetics (2001)
1047. BXD Recombinant Inbred Mice Represent a Novel Immune Response Model to AAV-F.IX
Authors: Huang-Ge Zhang, Hui-Chen Hsu, PingAr Yang, Qi Wu, Shaohua Yu, Jianhua Wang, Alexander Schlachterman, Katherine A. High, John D. Mountz
Journal: Molecular Therapy (2004)

The immune response to adeno-associated virus vector (AAV) gene therapy using the coagulation factor IX transgene (AAV-F.IX) can lead to either elimination of the vector or production of inhibitor antibodies to the expressed transgene. Interestingly not all individuals treated with F.IX or animal models of AAV-FIX develop such an immune response. AAV-F.IX gene therapy can also elicit a tolerance response, or no immune response to AAV and/or the F.IX transgene. We hypothesize that the combined effects of genetic diversity and multiple redundant pathways lead to a diverse antibody response to AAV and FIX. This was tested using C57BL/6 xDBA/2 (BXD) recombinant inbred (RI) strains derived from high responder DBA/2 and low responder C57BL/6 parental mice. To determine the cellular and genetic basis for this response, we analyzed strains of C57BL/6 X DBA/2 (BXD) RI mice derived from high responder, D2, and low responder, B6, parental mice and have observed three response patterns to AAV-F.IX. An immunogenic response was associated with high expression of CD40 and BAFF receptor (BAFF-R) on B cells and CD40 ligand (CD40L) on T cells. This occurred in BXD strains 12, 28, 31, and 33, as well as in D2 strain. A tolerogenic response was associated with development of a CD4+CD25+ T regulatory population. This population was strongly evident in BXD strains 6 and 25 at days 18 and 40, after AAV-F.IX. In certain strains, including BXD strains 8, 19, 22, and 23, exhibited no immune response, which was associated with low CD40 and low BAFF-R on B cells. Genetic linkage analysis revealed a highly significant locus at D12Mit150 (59 cM, LRS=42.1), near the Igh (59 cM) and CD40 binding protein (Traf3) loci (55 cM), and a suggestive locus at D17Mit36 (23 cM, LRS=18.1), near the MHC locus. These results indicate that the antibody response to AAV-F.IX exhibited: (i) a predictable strain-dependent pattern in BXD RI strains; (ii) a correlation with expression of CD40-CD40L, BAFF-BAFF-R, and % of CD4+CD25+ T cells; and (iii) significant linkage to a locus on Chr 12 near the Traf3/Igh locus, and on Chr 17 near the MHC locus.
Abstract 17597: Endogenous Del-1 is a Negative Regulator of Ischemia-induced Angiogenesis by Interacting With the Leukocytic LFA-1 Integrin
Authors: Sebastian Cremer, Anne Klotzsche-von Ameln, Alessia Orlandi, Irina Korovina, Bettina Gercken, Andreas M Zeiher, Stefanie Dimmeler, Triantafyllos Chavakis, Emmanouil Chavakis
Journal: Circulation (2014)

Developmental endothelial locus-1 (Del-1) is an endothelial cell-derived secreted protein circulating in blood and associated with the cell surface and the extracellular matrix. As we previously demonstrated, Del-1 restricts leukocyte recruitment by inhibiting the β2-integrin, LFA-1. Leukocytes and progenitor cells (PC) may contribute to angiogenesis. The role of endogenous Del-1 in angiogenesis is elusive. We found, that physiological angiogenesis of the developing retina was not affected in the Del-1-/- mice compared to the wildtype (WT) mice. Surprisingly, Del-1-/- mice displayed a significantly increased angiogenic response compared to WT mice after induction of hind limb ischemia (144 ± 6 % increase of capillary density) and retinal ischemia (retinopathy of prematurity model) suggesting that endogenous Del-1 is an inhibitor of ischemia-induced neovascularization. Silencing of Del-1 with siRNA did not affect the angiogenic sprouting of endothelial cell (EC) spheroids, indicating that Del-1 blocks angiogenesis in a non-endothelial cell autonomous pathway. Soluble Del-1 blocked the adhesion of inflammatory cells on EC monolayers. In line with these results, ischemic muscles and ischemic retinae from Del-1-/- mice displayed an enhanced infiltration with inflammatory cells compared to the WT mice. Since Del-1 blocks inflammatory cell homing by inhibiting the leukocytic LFA-1-integrin, we addressed the role of the Del-1/LFA-1-integrin interaction on the inhibitory function of endogenous Del-1 on angiogenesis. Indeed, Del-1/LFA-1-double deficiency reversed the pro-angiogenic phenotype of the Del-1-/- mice to the level of WT mice in the model of hind limb ischemia. Thus, the inhibitory role of Del-1 on neovascularization is mediated by the interaction of Del-1 with the LFA-1-integrin. Moreover, Del-1-deficiency led to an increased homing of intravenously injected murine fluorescence-labeled WT Lin- BM PC in ischemic muscles in comparison to WT mice after the induction of hind limb ischemia. Taken together, Del-1 acts as a negative regulator of ischemia-induced angiogenesis by interacting with the LFA-1-integrin expressed in hematopoietic cells, thereby inhibiting the homing of hematopoietic cells to ischemic tissues.
EXTREMELY HIGH LYMPHOCYTE COUNT IN LYMPHATIC LEUKEMIA: REPORT OF A CASE
Authors: R L HADEN, U V PORTMANN
Journal: Annals of Internal Medicine (1950)

Case Reports1 September 1950EXTREMELY HIGH LYMPHOCYTE COUNT IN LYMPHATIC LEUKEMIA: REPORT OF A CASERUSSELL L. HADEN, M.D., F.A.C.P., U. V. PORTMANN, M.D.RUSSELL L. HADEN, M.D., F.A.C.P., U. V. PORTMANN, M.D.Author, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-33-3-717 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptSeveral cases of leukemia demonstrating white cell counts as high as 1,000,000 have been reported. According to Forkner,1 "the actual number of leukocytes may vary from a few thousand to a million per cu. mm. of blood." Patients with myeloid leukemia and white blood counts approaching 1,000,000 have been seen at the Cleveland Clinic, but we have found no reference to cases with a white cell count of over 2,000,000, such as the one here presented.CASE REPORTA white man, aged 53, was admitted to the Cleveland Clinic on February 28, 1938, complaining of "gas" in the abdomen, aching...Bibliography1. Forkner CE: Leukemia and allied disorders, 1938, The Macmillan Company, New York, p. 67. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: RUSSELL L. HADEN, M.D., F.A.C.P.; U. V. PORTMANN, M.D.Affiliations: Cleveland, Ohio*Received for publication February 3, 1948.From the Departments of Internal Medicine and Therapeutic Radiology, Cleveland Clinic, Cleveland, Ohio. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 September 1950Volume 33, Issue 3Page: 717-718KeywordsBloodBlood cellsBlood countsDiagnostic radiologyLymphocytesLymphocytic leukemia ePublished: 1 December 2008 Issue Published: 1 September 1950 PDF downloadLoading ...