tbuckley/cabot-search huggingface dataset:
242. A Novel MiniDys-eGFP Fusion Gene for Developing Cell-Based Therapies of Duchenne Muscular Dystrophy
Authors: Sheng Li, En Kimura, Leonard Meuse, Xin Ye, Brent Fall, Steven D. Hauschka, John Faulkner, Jeffrey S. Chamberlain
Journal: Molecular Therapy (2004)

Duchenne muscular dystrophy (DMD) results from mutations in the largest known gene, dystrophin. Gene therapy for DMD will require methods to systemically deliver a therapeutic dystrophin gene to widely distributed muscles. Recent observations indicating that vascularly transplanted adult stem cells can home and become muscle cells in an appropriate micro-environment has suggested one possible method for delivering genes to muscle. A smaller, easily traceable, and functional dystrophin would facilitate experiments aimed at improving ex vivo cell-based strategies. We previously found that large portions of the dystrophin central rod domain (Hinge 2 to Repeat 19) and the C-terminal domain (encoded on exons 71 to Exon 78) are not essential for the full function of dystrophin, as assayed by the ability to prevent and partially reverse morphological and fucnional abnormalities in mdx mouse skeletal muscles. Here, we engineered a 5.7-kb MiniDys-eGFP fusion gene by replacing the C-terminal domain of mini-dystrophin (DH2-R19) with an enhanced green fluorescence gene coding sequence and by trimming down the size of 5' and 3' UTR of dystrophin. This fusion gene can be inserted into lentiviral vectors and efficiently delivered into a variety of cell types in vitro. This MiniDys-eGFP fusion protein was found to fully prevent dystrophy of multiple skeletal muscles in transgenic mdx mice carrying the fusion gene under the control of the human a-skeletal actin (HSA) gene promoter. This green fusion protein was easily observed by fluorescence microscopy on the sarcolemma of skeletal muscle fibers of the transgenic mdx mice. Furthermore, donor-derived GFP-positive myofibers were detected in mdx recipient muscles transplanted with either whole bone marrow cells or primary myoblasts isolated from the MiniDys-eGFP transgenic mice. These data indicate that the smaller, easily traceable, and functional MiniDys-eGFP will be useful for developing ex vivo cell-based gene therapies for DMD.
Abstract P271: Relationship among Dietary Patterns, Apolipoproteins, C Reactive Protein and Other Lipids in Adult Populations in Four Cities of the Southern Cone of Latin America
Authors: Rosana Poggio, Natalia Elorriaga, Laura Gutierrez, Vilma Irazola, Adolfo Rubinstein
Journal: Circulation (2014)

Background: In the last decades, the nutrition transition in Latin America turned to diets with higher total caloric intake and lower consumption of vegetables, cereals and fruits. An unhealthy dietary pattern affects plasma levels of apolipoprotein (Apo) AI, Apo B and C-reactive protein (CRP) which are independently associated with the incidence of coronary heart disease and stroke). There is no data regarding these biomarkers in relation to dietary patterns from the Southern Cone of Latin America. Therefore the objective of this study is to analyze these biomarkers in relation to our traditional dietary patterns. Methods: CESCAS I study is an observational prospective cohort study with a multistage probabilistic sample of 7,600 participants from general population of 4 sites in Argentina, Chile and Uruguay. We conducted a cross-sectional analysis in a randomly selected subsample of 988 subjects. Subjects with previous cardiovascular event or under lipid lowering agents were excluded. Diet was assessed using a previously validated 126-item food frequency questionnaire. We used a factor analysis to derive the major dietary patterns by gender. Linear regression model were used to examine the independent associations among Apo AI, B, CRP, other lipids by tertiles of dietary pattern scores. Results: The population sample included 418 men and 570 women. Higher scores of “Prudent pattern”, characterized by intake of fruits, vegetables, low fat dairy products, fish, seafood and whole cereal was independently and significantly associated in men with the reduction of Apo B (β -4,4), low density lipoprotein (LDL) (β -7,2), Apo B/A ratio (β -0,03) and a non-significant reduction of CRP (β -0,6). In women, we observed a significant reduction in Apo B (β -1,3), LDL (β -2,1), total cholesterol (TC) /high density lipoprotein (HDL) ratio (β -0,1) and a non-significant reduction of triglycerides (β -4 mg/dl). The “Western like pattern” (WLP) was characterized by meat, processed foods, pizza, empanadas, sweets and desserts, refined grains, vegetables oils and other fats. Higher scores of WLP showed in men a non-significant increment of Apo B (β 0,7), TC/HDL ratio (β 0,1), CRP (β 0,1), LDL (β 3), triglycerides (β 1,3) and a non-significant reduction of Apo AI (β -1,7) and HDL (β -0,7). In women, we observed a significant increment of TC/HDL ratio (β 0,2) and triglycerides (β 12). We also found a non-significant increment of Apo B and LDL and to reduce HDL and CRP. Conclusion: Our findings confirm that diet has an important role on health. Prudent diet pattern was associated with a healthier lipid profile especially in men. The WLP showed a trend to adversely affect the lipids profile and CRP.
Abstract 18727: RAGE-HMGB-1 Expression in the Ascending Aorta of Bicuspid Aortic Valve (BAV) Patients Correlates With Circulating SRAGE and May Predict Vascular Dysfunction Associated With BAV Disease
Authors: Emanuela Branchetti, Joseph E Bavaria, Juan Grau, Aseem Updhayay, Eric Lai, Richard Shaw, Joseph Gorman, Robert Gorman, Giovanni Ferrari
Journal: Circulation (2014)

Introduction: Bicuspid Aortic Valve (BAV) is associated with premature valve and vascular complications. Thirty to 50% of BAV patients require surgery for valve diseases and/or ascending aorta (AA) dilatation. Increasing studies suggest that the measure of AA diameter, as criteria for aortic surgery, is an imperfect predictor of dissection and rupture. Therefore there is a need to identify reliable markers of dysfunctional AA to guide the decision for surgery. Recently, Advanced glycation end products (AGEs), such as HMGB-1 and S100A12, and their binding to the Receptor for AGE (RAGE) have been associated to mechanisms of valve and vascular degeneration. We previously demonstrated that BAV patients have higher plasma levels of the soluble form of RAGE (sRAGE) when compared with tricuspid aortic valve (TAV) patients. We also showed that the AA of BAV patients with higher circulating sRAGE is characterized by severe elastin degradation and matrix disorganization when compared to those with lower sRAGE. Hypothesis: We hypothesized that the level of circulating sRAGE may be related to the expression of AGEs and RAGE in the AA. Methods: HMGB-1 and S100A12 plasma values were measured by ELISA in BAV (N=20) and TAV (N=20) patients previously tested for sRAGE. Logistic regression and multivariate analysis were performed. AA tissue from BAV patients with sRAGE concentration ranging from 491 pg/mL to 5978 pg/mL were tested for the expression of RAGE, HMGB-1 and S100A12 by immunohistochemistry and Western blotting. Results: RAGE expression linearly increased in the AA of BAV patients with higher plasma sRAGE (R2=0.5). RAGE and sRAGE levels correlate with the expression of HMGB-1. S100A12 was minimally detected and restricted to inflammatory infiltrates in both BAV and TAV patients with AA dilatation. Plasma level of S100A12 and HMGB-1 were not significantly different in BAV patients when compared to TAV and did not correlate with sRAGE concentration. Conclusions: These results suggest that HMGB1-mediated RAGE activation in the AA of BAV patients may be responsible for tissue degeneration and increased sRAGE release in the bloodstream. The interaction AGE-RAGE in the AA of BAV patients may help in evaluating the risk for rapid AA degeneration independently of dilation.
Histone deacetylase 4 promotes cholestatic liver injury in the absence of prohibitin‐1
Authors: Lucía Barbier‐Torres, Naiara Beraza, Pablo Fernández‐Tussy, Fernando Lopitz‐Otsoa, David Fernández‐Ramos, Imanol Zubiete‐Franco, Marta Varela‐Rey, Teresa C. Delgado, Virginia Gutiérrez, Juan Anguita, Albert Pares, Jesús M. Banales, Erica Villa, Juan Caballería, Luis Alvarez, Shelly C. Lu, Jose M. Mato, María Luz Martínez‐Chantar
Journal: Hepatology (2015)

Prohibitin‐1 (PHB1) is an evolutionarily conserved pleiotropic protein that participates in diverse processes depending on its subcellular localization and interactome. Recent data have indicated a diverse role for PHB1 in the pathogenesis of obesity, cancer, and inflammatory bowel disease, among others. Data presented here suggest that PHB1 is also linked to cholestatic liver disease. Expression of PHB1 is markedly reduced in patients with primary biliary cirrhosis and biliary atresia or with Alagille syndrome, two major pediatric cholestatic conditions. In the experimental model of bile duct ligation, silencing of PHB1 induced liver fibrosis, reduced animal survival, and induced bile duct proliferation. Importantly, the modulatory effect of PHB1 is not dependent on its known mitochondrial function. Also, PHB1 interacts with histone deacetylase 4 (HDAC4) in the presence of bile acids. Hence, PHB1 depletion leads to increased nuclear HDAC4 content and its associated epigenetic changes. Remarkably, HDAC4 silencing and the administration of the HDAC inhibitor parthenolide during obstructive cholestasis in vivo promote genomic reprogramming, leading to regression of the fibrotic phenotype in liver‐specific Phb1 knockout mice. Conclusion : PHB1 is an important mediator of cholestatic liver injury that regulates the activity of HDAC4, which controls specific epigenetic markers; these results identify potential novel strategies to treat liver injury and fibrosis, particularly as a consequence of chronic cholestasis. (H epatology 2015;62:1237‐1248)
Nachbehandlung nach chirurgischen Eingriffen
Authors: Eduard Melchior
Journal: JAMA (1934)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Bosentan is superior to ambrisentan in reducing the expression of asthma- and COPD-related cytokines
Authors: Jurgen Knobloch, Yingfeng Lin, David Jungck, Katja Urban, Justus Strauch, Erich Stoelben, Juergen Behr, Andrea Koch
Journal: European Respiratory Journal (2011)

TNFa substantially contributes to the establishment of chronic airway inflammation. TNFa-induced expression of inflammatory genes in human airway smooth muscle cells (HASMCs) might depend on endothelin-1 (ET-1) signaling.  We investigated inflammatory gene expression in TNFa-exposed HASMCs. We compared the anti-inflammatory effects of the non-selective endothelin receptor A (ETAR)/ETBR antagonist Bosentan with those of the ETAR-selective antagonist Ambrisentan.  The overall response of cultivated HASMCs of n=8 current smokers to TNFa in the absence or presence of Bosentan or Ambrisentan was investigated by gene expression analysis with the Agilent Whole Genome Oligo Microarray technique (40,000 genes). Results of Asthma- and/or COPD-related cytokines/chemokines were verified by quantitative RT-PCR and ELISA.  The expression of 4,948±316 genes was induced twofold or more by TNFα. Among them were GM-CSF, G-CSF, 8 CC and 8 CXC family members and 5 interleukins. Bosentan and Ambrisentan reduced the expression of 310±51 or 396±63 genes, respectively, in TNFα-exposed HASMCs. Among them were CCL2/5/7/8/19/20, CXCL6/10, CX3CL1, IL-6/7/23 and GM-CSF (all p  HASMCs contribute to the establishment of chronic airway inflammation in asthma and COPD. Particularly none-selective ET receptor antagonists might have therapeutic utility in early stages of chronic airway diseases by counteracting the establishment of inflammatory processes.
Development of liposomal anthracyclines: from basics to clinical applications1This paper is based on a lecture presented at the 8th International Symposium on recent Advances in Drug Delivery Systems (Salt Lake City, UT, USA, 1997).1
Authors: Alberto Gabizon, Dorit Goren, Rivka Cohen, Yechezkel Barenholz
Journal: Journal of Controlled Release (1998)
Abstract 1917: Re-thinking the concept of cancer stem cells: Polyploid giant cancer cells as mother cancer stem cells
Authors: Jinsong Liu, Imelda Mercado-Uribe, Na Niu, Baocun Sun, Jian Kuang, Shiwu Zhang
Journal: Cancer Research (2014)

Abstract Polyploid giant cancer cells (PGCCs) have been observed by pathologists for over a century. PGCCs contribute to solid tumor heterogeneity, but their functions are largely undefined. Little attention has been given to these cells, largely because PGCCs have been generally thought to originate from repeated mitosis/cytokinesis failure and have no capacity for long-term survival and cell proliferation. Our group has recently reported that we successfully purified and cultured PGCCs from human ovarian cancer cell lines and primary ovarian cancer. These cells are highly resistant to oxygen deprivation and could form through endoreduplication or cell fusion, generating regular-sized cancer cells quickly through budding or burst. They are positive for normal and cancer stem cell markers, divided asymmetrically and cycled slowly. They can differentiate into adipose, cartilage, and bone. A single PGCC formed cancer spheroids in vitro and generated tumors in immunodeficient mice. PGCC-derived tumor gained a mesenchymal phenotype with increased expression of cancer stem cell markers CD44 and CD133 and become more resistant to the treatment of cisplatin and also form tissue organ-like structure. Together, our results reveal that the PGCCs present a resistant form of human cancer that behaves like mother cancer stem cells that can contribute to generation of daughter cancer stem-like cells and formation of tissue organ structure. These cells can be induced in response to hypoxia and play a fundamental role in regulating tumor heterogeneity, stemness, and chemoresistance in human cancer. In contrast to the phenotype unstable cancer stem cells selected by cell surface markers, PGCCs can be stably passaged and maintained, thus these cells provide reproducible experimental system to study mechanisms involved in generation of cancer stem cells. The key references related this concept are listed below. we will provide a most updated data to support this new concept that PGCCs as mother cancer stem cells at this meeting. Zhang S et al, Oncogene 2013, March online publication; Zhang S et al, Int J Cancer. 2013 Feb 1;134(3):508-18; Zhang S el al, Cancer Lett. 2013 Jun 10;333(2):205-12. Citation Format: Jinsong Liu, Imelda Mercado-Uribe, Na Niu, Baocun Sun, Jian Kuang, Shiwu Zhang. Re-thinking the concept of cancer stem cells: Polyploid giant cancer cells as mother cancer stem cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1917. doi:10.1158/1538-7445.AM2014-1917
PROGRESSIVE PULMONARY HISTOPLASMOSIS WITH BILATERAL RESECTION AND CHEMOTHERAPY
Authors: C R HOWSON, L A BREWER, W H OATWAY, E A ROUFF
Journal: Annals of Internal Medicine (1956)

Case Reports1 May 1956PROGRESSIVE PULMONARY HISTOPLASMOSIS WITH BILATERAL RESECTION AND CHEMOTHERAPYCARL R. HOWSON, M.D., F.A.C.P., LYMAN A. BREWER III, M.D., F.A.C.S., WILLIAM H. OATWAY JR., M.D., ELLIOT A. ROUFF, M.D.CARL R. HOWSON, M.D., F.A.C.P.Search for more papers by this author, LYMAN A. BREWER III, M.D., F.A.C.S.Search for more papers by this author, WILLIAM H. OATWAY JR., M.D.Search for more papers by this author, ELLIOT A. ROUFF, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-44-5-985 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptPulmonary histoplasmosis, like pulmonary coccidioidomycosis, may simulate tuberculosis so closely that the differentiation on physical and radiologic examination may be impossible. While comparatively little is known as to the extent of its geographic dissemination, there is good reason to believe that it is much more widespread than has been suspected. Its recognition is therefore of increasing importance.The case reported here presents several aspects which, to the best of our knowledge, have not yet been mentioned in the literature.CASE REPORTA white male, age 38, employed as foreman of an oil well supply company, first consulted a thoracic surgeon...Bibliography1. FurcolowGrayston MLJT: Occurrence of histoplasmosis in epidemics, Am. Rev. Tuberc. 68: 307, 1953. MedlineGoogle Scholar2. Tucker HA: Histoplasmosis on the Isthmus of Panama, Am. J. Trop. Med. and Hyg. 1: 462, 1952. CrossrefMedlineGoogle Scholar3. PriorSaslowCole JASCR: Experiences with histoplasmosis, Ann. Int. Med. 40: 221, 1954. LinkGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Los Angeles, California*Received for publication June 20 1955.†Unless otherwise stated, the laboratory studies were conducted by R. A. Hammack, M. D. and associates. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByThe therapy of cavitary pulmonary histoplasmosisSURGICAL THERAPY OF CHRONIC PULMONARY HISTOPLASMOSIS WITH AND WITHOUT AMPHOTERICIN BChronic active pulmonary histoplasmosis with cavitationExperiences with the Therapy of Sixty Cases of Deep Mycotic Infections 1 May 1956Volume 44, Issue 5Page: 985-993KeywordsCoccidioidomycosisHistoplasma capsulatumHistoplasmosisLesionsOilsPneumoniaProteinsResearch laboratoriesSurgeonsTuberculosis Issue Published: 1 May 1956 PDF DownloadLoading ...
Eye care utilization by older americans
Authors: Peggy Orr, Yolanda Barrón, Oliver D Schein, Gary S Rubin, Sheila K West
Journal: Ophthalmology (1999)

ObjectiveTo determine eye care utilization patterns among older Americans, particularly characterizing those who sought different types of providers, and the predictive factors for seeking eye care services in general and among those with diabetes and those with visual loss.Design and participantsThe SEE Project, a population-based survey of 2520 persons aged 65 to 84 in Salisbury, Maryland, provided cross-sectional data on eye care use. Questions on eye care use, demographics, medical history, and other factors were asked on the home interview.Main outcome measuresUse of an eye care provider in the previous year, with additional outcomes of use of different types of eye care providers.ResultsBlacks were significantly less likely to see any type of eye care provider over 1 year: 50% versus 69% among whites. Those who reported having a vision problem, those with more education, and those in the older age groups were significantly more likely to see either an ophthalmologist or an optometrist. Diabetes and driving a car were predictive factors for seeing an ophthalmologist but not for seeing an optometrist. Self-report of diabetes and eye care problems, and being a current driver, were predictive of seeing an eye care professional among those with visual impairment.ConclusionsAlthough blacks are known to be at greater risk for several age-related eye diseases, they are much less likely to see an eye care provider. Interventions that remove barriers to eye care services should be considered.
Tumor Promotion by Citrus Oils: Tumors of the Skin and Urethral Orifice in Mice&lt;xref ref-type="fn" rid="FN1"&gt;2&lt;/xref&gt;
Authors: Francis J. C. Roe, Winifred E. H. Peirce
Journal: JNCI Journal of the National Cancer Institute (1960)

Journal Article Tumor Promotion by Citrus Oils: Tumors of the Skin and Urethral Orifice in Mice Get access Francis J. C. Roe, Francis J. C. Roe Cancer Research Department, London Hospital Medical College, London, England Search for other works by this author on: Oxford Academic PubMed Google Scholar Winifred E. H. Peirce Winifred E. H. Peirce Cancer Research Department, London Hospital Medical College, London, England Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 24, Issue 6, June 1960, Pages 1389–1403, https://doi.org/10.1093/jnci/24.6.1389 Published: 01 June 1960 Article history Received: 30 November 1959 Revision received: 22 January 1960 Published: 01 June 1960
Modulation of Cytokine Release and Neutrophil Function by Granulocyte Colony-Stimulating Factor During Endotoxemia in Humans
Authors: Dasja Pajkrt, Annemieke Manten, Tom van der Poll, Monique M.C. Tiel-van Buul, Jaap Jansen, Jan Wouter ten Cate, Sander J.H. van Deventer
Journal: Blood (1997)
Abstract 52: The Heart of the Matter: Cardiac Arrest and Public Health Information on Twitter
Authors: Justin C Bosley, Nina C Zhao, David A Asch, Lance Becker, Raina M Merchant
Journal: Circulation (2011)

Background: Despite the increasing use of social media, little is known about how it is used to communicate health-related information. Twitter's 300 million users communicate a variety of information via their “tweets” (140 character messages). We sought to characterize the cardiac arrest specific information being shared. Methods: A Twitter search engine was used to identify tweets published April 19-May 19 2011 with keywords: “cardiac arrest, CPR, AED, resuscitation, heart arrest, and defib.” A 1% sample of tweets was reviewed independently by three study investigators to characterize content. Tweets were then categorized as relating to cardiac arrest (personal sharing/ information sharing), CPR or AED (personal sharing/ information sharing), health education/news media, or unrelated to resuscitation (e.g., jokes, miscellaneous). Results: Of 61,053 analyzed tweets, 25% (15,324) included cardiac arrest specific information, (i.e. not miscellaneous). Of this cohort: 14% of tweets referenced cardiac arrest events, 29% referenced CPR performance/AED use, and 57% included cardiac arrest related health education/news media references or links. Of tweets referencing cardiac arrest events (n=2,172), 5% represented personal sharing (e.g. “when I or a family member/friend/ had a cardiac arrest”) and 9% represented information sharing (e.g. arrest location, interventions, guidelines). Of tweets referencing CPR performance or AED use (n=4,465), 23% represented personal sharing (e.g. actual or classroom provision of CPR/AED, likes/dislikes regarding CPR/AED courses) and 6% represented information sharing (e.g., observation of CPR delivery or AED use, commentary regarding hands-only CPR). Tweets (n=8,687) regarding resuscitation specific education or news articles included content such as: advocacy group events, CPR/AED training events, heart health surveys, and reports of celebrities, athletes, and young adults affected by cardiac arrest. Conclusion: Cardiac arrest related information is discussed on Twitter in a variety of categories, with unique opportunities for healthcare provider information sharing, therapeutic intervention guidance (e.g., CPR/AED provision, therapeutic hypothermia), and medical education.