tbuckley/cabot-search huggingface dataset:
Late asthmatic response is modulated by TRPA1 antagonists in ovalbumin-induced bronchoconstriction in anaesthetized guinea pigs
Authors: Thierry Bouyssou, Zoe Noakes, Silke Hobbie, Martin Fleck, Andreas Schnapp, Florian Gantner
Journal: European Respiratory Journal (2012)

In animal models of asthma, ovalbumin (OVA) aerosol results in bronchoconstriction characterized by a histamine-related early asthmatic response (EAR) followed by a neuropeptide-related late asthmatic response (LAR) which can be modulated by transient receptor potential (TRP) channel A1 antagonists (Thorax. 2012, 67, 19-25).  The aim of the study was to assess the potential of two TRPA1 antagonists on the EAR and LAR in OVA-induced bronchoconstriction in anaesthetized guinea pigs.  Bronchoconstriction was induced by intra-tracheal administration of a single dose of OVA (50 µg/kg) and lung resistance recorded for 30 minutes. The animals were pre-treated with pyrilamine (2 mg/kg i.v.) or its vehicle (saline) 10 min before OVA. The TRPA1 antagonists HC-030031 and A-967079 or their vehicle (0.5 % methylcellulose) were administered i.p. 1 h before OVA. Without pyrilamine pre-treatment, OVA induced a fast increase in lung resistance (max. 60 ± 13 ml overflow after 1 min) which was not reduced by the TRPA1 antagonists. Under pyrilamine pre-treatment, OVA induced a slow increase in lung resistance (max. 33 ± 10 ml overflow at the end of the recording). HC-030031 (1 µg/kg – 1 mg/kg) dose-dependently inhibited the non-histamine-related OVA- induced bronchoconstriction (ED 50  = 0.01 mg/kg) with a maximum bronchoprotection of 76 % at 0.03 mg/kg (p 50  = 0.01 mg/kg) with a maximum bronchoprotection of 78 % at 0.03 mg/kg (p  This study shows that the EAR is histamine related, while the LAR is modulated by the TRPA1 channel in OVA-induced bronchoconstriction in anaesthetized guinea pigs.
National survey of physicians practice patterns: Fertility preservation and cancer patients
Authors: G. Quinn, S. T. Vadaparampil, P. Jacobsen, J. Lee, J. Lancaster, G. Bepler, D. L. Keefe, T. L. Albrecht
Journal: Journal of Clinical Oncology (2009)

CRA9508 The full, final text of this abstract will be available in Part II of the 2009 ASCO Annual Meeting Proceedings, distributed onsite at the Meeting on May 30, 2009, and as a supplement to the June 20, 2009, issue of the Journal of Clinical Oncology. No significant financial relationships to disclose.
Orotracheal instillation of gastric juice: Species variations in acute lung injury relate to differences in matrix metalloproteinase activity
Authors: Gisella Borzone, Pedro Ayala, Joaquin Araos, Rafael Contreras, Andrea Cutiño, Manuel Meneses
Journal: European Respiratory Journal (2014)

Gastric content aspiration is common in the elderly and people with neurological conditions. It is unknown if early events predict final outcomes after aspiration. To gain further knowledge we studied rat and hamster species variations in acute lung injury (ALI) by a single orotracheal dose of gastric juice (GJ). Under Ketamine/Xylazine, Sprague-Dawley rats and Syrian Golden hamsters received 1.5ml/Kg body wt. of GJ from a pool for each species (similar pH, pepsin activity and particle type). ALI markers (lung wet/dry weight ratio, histological changes, and BALF protein content) and lung matrix metalloproteinase-9 (MMP-9) activity were measured 4, 12, 24h, and 4 days(d) later (n=10/time point/species). Statistical analysis: ANOVA.  In rats, lung wet/dry weight ratio increased at 4h (p  Conclusion: ALI by a single dose of GJ is more severe in hamsters than in rats and is associated with a long-lasting increase in MMP-9 activity. Early species variations in MMP-9 activity could be associated with species differences in chronic damage if aspiration events become repetitive.  Funding: FONDECYT 1120943.
Sponsorship, Authorship, and Accountability
Authors: Davidoff F, DeAngelis Cd, Drazen Jm, Nicholls Mg, Hoey J, Højgaard L, Horton R, Kotzin S, Nylenna M, Overbeke Aj, Sox Hc, Van Der Weyden Mb, Wilkes Ms
Journal: New England Journal of Medicine (2001)

As editors of general medical journals, we recognize that the publication of clinical-research findings in respected peer-reviewed journals is the ultimate basis for most treatment decisions. Public discourse about this published evidence of efficacy and safety rests on the assumption that clinical-
Frequent Dysregulation of the c-maf Proto-Oncogene at 16q23 by Translocation to an Ig Locus in Multiple Myeloma
Authors: Marta Chesi, P. Leif Bergsagel, Oluwatoyin O. Shonukan, Maria Luisa Martelli, Leslie A. Brents, Theresa Chen, Evelin Schröck, Thomas Ried, W. Michael Kuehl
Journal: Blood (1998)
Occupational asthma in two healthcare workers due to an alcohol hand gel
Authors: Alastair Robertson, Vicky Moore, Cedd Burge, Marcus Wong, Sherwood Burge
Journal: European Respiratory Journal (2011)

Alcohol hand gels are increasingly being used by healthcare workers to reduce the risk of hospital-acquired infection. To date they have not been identified as a cause of occupational asthma in healthcare workers. We present two cases of occupational asthma in healthcare workers due to an alcohol hand gel containing a quaternary ammonium bittering agent (denatonium).  Two healthcare workers, a midwife (case 1) and a nurse (case 2), presented with asthma improving on days away from work. Both had developed symptoms in relationship to using the same alcohol hand rub. Both workers completed serial peak flow measures which showed occupational asthma (case 1 OASYS score 3.43, case 2 OASYS score 3.93). A series of bronchial challenge tests were performed to the hand gel and to a control agent. Case 1 had a dual asthmatic reaction and case 2 had a prolonged immediate reaction to the hand gel. Both workers improved on removal from exposure.  Hand gels containing denatonium are used extensively by healthcare workers. There needs to be an awareness of this agent as a potential cause of occupational asthma in this group of workers.
A clinical, hematologic, and immunologic analysis of 21 HTLV-II- infected intravenous drug users [published erratum appears in Blood 1990 Nov 1;76(9):1901]
Authors: JD Rosenblatt, S Plaeger-Marshall, JV Giorgi, P Swanson, IS Chen, E Chin, HJ Wang, M Canavaggio, MA Hausner, AC Black
Journal: Blood (1990)
Occupational asthma to quaternary ammonium compounds: Clinical features of 16 positive bronchial challenge tests using didecyl dimethyl ammonium chloride
Authors: Jean-Marie Renaudin, Frédéric de Blay, Brigitte Sbinné
Journal: European Respiratory Journal (2012)

Introduction: Quaternary ammonium compounds (QACs) are used as detergents or disinfectants and known to induce occupational asthma. Moreover, clinical reports with bronchial challenge tests (BCTs) that demonstrated the role of QACs are rare.  Objectives: To analyse the clinical response obtained during a positive BCT in 16 patients with occupational asthma to QACs.  Methods: A realistic BCT was performed in an experimental chamber with didecyl dimethyl ammonium chloride (DDAC) at 0.1%. This concentration corresponded to the lowest concentration of DDAC to which patients were exposed at workplace. A positive BCT was defined as a 20% drop in FEV 1  or a change of 2 doubling dose in non-specific BCT to methacholine.  Results: 14 women and 2 men (mean age = 47.8 y.) were included from 2008 to 2011. 11 of them (62.5%) were healthcare workers: 1 dentist, 1 dentist-assistant, 2 nurses, 5 auxiliary nurses, 1 janitor. 5 of them (37.5%) were cleaners. 69% had also occupational rhinitis and 19% occupational contact dermatitis to QACs. The mean duration of exposure during BCT was 28 minutes [3-60]. Same BCTs were negative in 6 control patients with work-related asthma due to another cause and exposed to QACs.  Conclusions: Our study demonstrated the usefulness of BCT to confirm an occupational asthma to QACs. As compared to previous publication from our group, bronchial response occurred more rapidly suggesting a sensitisation to DDAC.
Evaluation of lung high resolution Ct scan (HRCT) in PPD positive cases who had close contact with BK positive sputum smear patients
Authors: Kazem Navari, Farzaneh Navari, Najmeh Navari, Mahnaz Vaezi
Journal: European Respiratory Journal (2013)

Background: The goal of This study was to determine the Lung HRCT Scan finding in the PPD positive (PPD>10mm) cases of the family members or person who had close contact and with positive BK sputum Smear of pulmonary TB Patients.  Methods:This was a prospective cohort study on 38 cases PPD Positive who had close contact with positive BK sputum smear from Sep- 2009 to sep2012 In Bandar Abbas,Iran.we checked ESR, smoking,PPD size,AGE and SEX with Lung HRCT Scan Finding.Data were analyzed by software SPSS19 and involved both descriptive and inferential statistics including Chi-Square test and ANOVA.(P  Results:In this study Mean age 32±13,45% Male,55% Female and 37% were Smoker. Lung HRCT Scan finding.  No Significant correlation was not find between PPD size,ESR,AGE,Smoking and sex with Lung HRCT Scan Finding(P>0.05).  Conclusion: In PPD Positive Cases who had close contact with Positive BK Sputum Smear, Lung HRCT Scan findings has not correlation with ESR, PPD Size, Smoking, SEX and Age.
Targeting leukemia stem cells in vivo with antagomiR-126 nanoparticles in acute myeloid leukemia
Authors: A M Dorrance, P Neviani, G J Ferenchak, X Huang, D Nicolet, K S Maharry, H G Ozer, P Hoellarbauer, J Khalife, E B Hill, M Yadav, B N Bolon, R J Lee, L J Lee, C M Croce, R Garzon, M A Caligiuri, C D Bloomfield, G Marcucci
Journal: Leukemia (2015)
Reexamining the concept of adolescence: differences between adolescent boys and girls in the context of their families
Authors: J F McDermott, A B Robillard, W F Char, J Hsu, W S Tseng, G C Ashton
Journal: American Journal of Psychiatry (1983)

As part of a larger study on family functioning, the authors administered a questionnaire on individual attitudes toward family values to 158 Japanese-American and Caucasian families. Differences between the generations on questions of authority and responsibility were predictable; few differences were found between ethnic groups. However, differences were striking between adolescent boys and girls, regardless of ethnicity: Girls valued family affiliation, closeness, and emotional expression significantly more highly than did boys. The authors emphasize the need for families to value girls' needs for closeness and emotional expression as highly as boys' needs for independence and self-differentiation. They suggest that the concept of separation-individuation as the major goal of adolescence be reexamined.
X-linked thrombocytopenia identified by flow cytometric demonstration of defective Wiskott-Aldrich syndrome protein in lymphocytes
Authors: Hirokazu Kanegane, Keiko Nomura, Toshio Miyawaki, Yoji Sasahara, Shin Kawai, Shigeru Tsuchiya, Gyokei Murakami, Takeshi Futatani, Hans D. Ochs
Journal: Blood (2000)
Effects of ciclesonide on the management of stable COPD with airway eosinophilia
Authors: Hiroaki Kume, Osamu Nishiyama, Yuji Higashimoto, Hirokazu Nakajima, Yuji Tohda
Journal: European Respiratory Journal (2013)

Rationale:  Although neutrophil inflammation in airways is generally considered as the principle pathophysiology in COPD, eosinophil inflammation in the airways is observed in some patients with this disease independent of asthma. Little is currently unknown about not only mechanisms but also therapy in this clinical phenotype of COPD. This clinical study was designed to determine whether inhaled glucocorticosteroids (ICS) are useful for airway eosinophilia in COPD.   Methods:  In stable COPD by indacaterol (LABA), when a percentage of eosinophil (Eo) in the induction sputum is >3%, ciclesonide (ICS) was administered because this agent is delivered to distal airways due to smaller particle size. In contrast, ICS was not added when Eo value is    Results:  15 patients with COPD (GOLD 2-3) with airway eosinophilia were enrolled. Symptoms and lung function in all of these cases have been already improved by indacaterol. COPD Assessment Test (CAT) score was decreased to 7.2 and inspiratory capacity was increased by 310 mL. However, ciclesonide was added to 6 cases because of sputum Eo >3% (8.1%). After administration of ciclesonide, values of Eo and CAT were decreased to 2.1, and 4.8, respectively (each  P    Conclusions:  Not only LABA but also ICS is needed to achieve better maintenance in COPD with airway eosinophilia. Ciclesonide is effective for these cases. Eosinophil infiltration to airways is indication of ICS therapy for COPD.
Effect of (-)-gossypol (AT-101) on transcriptional regulation of Noxa and Puma and on Mcl-1-mediated cancer cell resistance to apoptosis
Authors: S. Wang, F. Jiang, L. Bai, J. Jianting Long, S. Qiu, J. Chen, D. McEachern
Journal: Journal of Clinical Oncology (2009)

e14611 Background: AT-101 is being evaluated in Phase II clinical trials for the treatment of human cancers and demonstrates anticancer activity as a single agent and in combination with docetaxel. Despite extensive investigations, the mechanism of action for its antitumor activity is not completely understood. In this study, we have performed detailed investigations using a panel of human cancer cell lines on the mechanism of action for AT-101. Methods: A series of human breast and prostate cancer cell lines were utilized to evaluate the antitumor activity of AT-101. Cell growth inhibition, cell viability and apoptosis were determined by WST assay, trypan blue assay and flow cytometry, respectively. Gene specific siRNAs were used to knockdown target gene expression. Quantitative RT- PCR was performed to detect the mRNA levels of Puma and Noxa. Co-immunoprecipitation was carried out to detect the association between Mcl-1 and Noxa or Puma. Results: AT-101 induced apoptosis in both Bax/Bak-dependent and -independent manners in a variety of human cancer cell lines concomitant with increased expression of Noxa and Puma in a p53-independent manner. The up-regulation of Noxa was not observed in normal primary breast epithelial cells or normal-like human breast epithelial MCF-12F cells. Transcription inhibitor actinomycin D blocked AT-101-induced up-regulation of Puma and Noxa. Knockdown of Noxa or Puma attenuated AT-101-induced apoptosis. Furthermore, AT-101 effectively overcame Mcl-1-mediated cancer cell resistance to apoptosis. Co-immunoprecipitation studies demonstrated that Mcl-1 specifically associated with Noxa but not Puma. Conclusions: Our findings suggest that transcriptional up-regulation of pro-apoptotic Noxa and Puma contributes to the antitumor activity of AT-101, which plays a dominant role in antagonizing Mcl-1 and overcoming Mcl-1-mediated resistance to apoptosis of cancer cells. AT-101 functions as an antagonist of Bcl-2, Bcl-xL and Mcl-1 through direct binding to these proteins, as well as through upregulation of Noxa and Puma. [Table: see text]
Screening instruments for psychiatric morbidity in chronic fatigue syndrome
Authors: Richard K Morriss, Alison J Wearden
Journal: Journal of the Royal Society of Medicine (1998)

Physicians require a screening instrument to detect psychiatric disorders in patients with chronic fatigue syndrome (CFS). Different threshold scores on the Hospital Anxiety and Depression scale (HAD) and the mental health scale of the Medical Outcome Survey (MOS) were compared with two gold standards for the presence or absence of psychiatric disorder, standard diagnostic criteria (DSM-III-R) and a threshold score for the number of psychiatric symptoms at a standardized psychiatric interview (Revised Clinical Interview Schedule total cut-off score of 11/12). They were compared by use of validating coefficients and receiver operating characteristics in 136 consecutive CFS medical outpatients. The HAD scale at cut-off of 9/10 was a valid and efficient screening instrument for anxiety and depression by comparison with both gold standards. The MOS mental health scale at its recommended cut-off score of 67/68 yielded too many false-positives to be recommended as a psychiatric screening instrument in CFS patients.
Abstract 11774: Bi-Directional Vascular Calcifying Progenitor Cells Differentiate Into Decalcifying Cells Via Ppar-γ Activation
Authors: Hyun-Ju Cho, Hyun-Jai Cho, Ho-Jae Lee, Myung-Kang Song, Ju-Young Kim, Hae-Young Lee, Bon-Kwon Koo, Byung-Hee Oh, Young-Bae Park, Hyo-Soo Kim
Journal: Circulation (2011)

Vascular calcification is an advanced feature of atherosclerosis. But, no effective therapy is available. We investigated the characteristics of vascular calcifying progenitor cells and their calcifying/ decalcifying potentials. Cells from aortas of C57 mice were sorted into 4 groups using Sca-1 and PDGFRα. Sca-1+ cells were clonally expanded from single cell. Sca-1+ cells were found to have greater osteoblastic differentiation potentials than Sca-1- cells. Interestingly, under RANKL and M-CSF treated condition, Sca-1+/PDGFRα-cells differentiated into osteoclasts 3 fold more than Sca-1+/PDGFRα+ cells (P ex vivo . Furthermore, PPARγ activation, even under osteoblastic differentiation-inducing conditions, enhanced the osteoclastic differentiation of Sca-1+/PDGFRα- cells. Next, we performed two kinds of  in vivo experiments; ectopic and atherosclerotic calcification. CT scoring of ectopic calcified nodules showed that the injection of Sca-1+/PDGFRα- cells increased bone volume and calcium score compared to PBS injection. Moreover, Sca-1+/PDGFRα- cells with PPARγ agonist decreased bone formation 2 fold, compared to Sca-1+/PDGFRα- cells without PPARγ agonist (P in vivo  bi-directional fate. These findings suggest that a subtype of BM-derived, vessel-residing progenitor cells offer a therapeutic target for the treatment of vascular calcification, and that PPARγ activation may provide a means of reversing calcification.
Abstract 3080: Sex Differences in Peripheral Arterial Disease
Authors: Amir H Sadrzadeh Rafie, Stacy T Sims, Kimberly A Edwards, Tiffany Phan, Marcia L Stefanick, Themistocles Assimes, Jennifer A Tremmel, Jeffrey Olin, John P Cooke
Journal: Circulation (2008)

Background: We aimed to determine whether sex differences exist in the distribution of peripheral arterial disease (PAD) in a group of patients referred for coronary angiography. Methods and Result...
Chemical castration induced by adjuvant cyclophosphamide, methotrexate, and fluorouracil chemotherapy causes rapid bone loss that is reduced by clodronate: a randomized study in premenopausal breast cancer patients.
Authors: T Saarto, C Blomqvist, M Välimäki, P Mäkelä, S Sarna, I Elomaa
Journal: Journal of Clinical Oncology (1997)

PURPOSE In the majority of premenopausal breast cancer patients, an adjuvant chemotherapy-induced early menopause occurs, which is known to be a strong predictor of osteoporosis. We present data on the effect of adjuvant cyclophosphamide, methotrexate, and fluorouracil (CMF) therapy on bone mineral density (BMD) and the efficacy of clodronate on the prevention of bone loss in 148 premenopausal breast cancer patients without skeletal metastases. MATERIALS AND METHODS Patients were randomized to receive oral clodronate 1,600 mg/d or to a control group. In addition, patients were treated with six cycles of CMF therapy. BMD of the lumbar spine and femoral neck was measured by dual-energy x-ray absorptiometry (DEXA) before therapy and at 1 and 2 years. RESULTS Changes in the BMD of lumbar spine and femoral neck were -5.9% and -2.0% without clodronate and -2.2% and +0.9% with clodronate at 2 years (P = .0005 and .017, respectively). Patients who developed amenorrhea after chemotherapy had a rapid bone loss, which was significantly reduced by clodronate. In controls, bone loss was 9.5% in the lumbar spine and 4.6% in the femoral neck, while in the clodronate group, bone loss was 5.9% and 0.4%, respectively, at 2 years. Patients with preserved menstruation had only marginal changes in BMD. CONCLUSION Chemotherapy-induced ovarian failure causes rapid bone loss in premenopausal breast cancer patients. Women older than 40 years are at particularly high risk. Clodronate significantly reduces this bone loss.
Importance of psychotic features to long-term course in major depressive disorder
Authors: W Coryell, A Leon, G Winokur, J Endicott, M Keller, H Akiskal, D Solomon
Journal: American Journal of Psychiatry (1996)

Most efforts to describe the prognostic significance of psychotic features in depression have been limited to single assessments 1 year or less after the initial evaluation. However, the various biological and treatment response differences between patients with psychotic and nonpsychotic depression suggest that prognostic differences may be very long-term.The 787 patients described here entered the study as they sought treatment at one of five academic medical centers; they had either RDC major depressive disorder or schizo-affective depression (other than the mainly schizophrenic subtype) and completed at least 6 months of follow-up. Of these, 144 (18.3%) had psychotic depression as defined here. Patients provided follow-up interviews at 6-month intervals for 5 years and annually thereafter; 98 of those with psychotic depression and 434 of those with non-psychotic depression were followed for 10 years.Those who began follow-up with psychotic depression had fewer weeks with minimal symptoms in each of the 10 years of follow-up and reported more psychosocial impairment at both 5 and 10 years. Both the index episode and the first recurrence of psychotic depression lasted longer than nonpsychotic episodes, but nonpsychotic episodes among previously psychotic individuals were relatively brief. Intervals between episodes were significantly shorter for patients who had ever been psychotic.Together with evidence that psychotic features are highly recurrent, these data show 1) that psychotic features denote a lifetime illness of greater severity and 2) that within individuals, psychotic features may emerge in only the more severe episodes.
Carcinogenicity of lindane, DDT, and 2,4-dichlorophenoxyacetic acid
Authors: Dana Loomis, Kathryn Guyton, Yann Grosse, Fatiha El Ghissasi, Véronique Bouvard, Lamia Benbrahim-Tallaa, Neela Guha, Heidi Mattock, Kurt Straif
Journal: The Lancet Oncology (2015)
Allopurinol Therapy of Gout with Renal Complications
Authors: K.-H. Sjoberg
Journal: Annals of the Rheumatic Diseases (1966)

We have determined the affinity and selectivity of a new nonpeptide antagonist PD156707 (sodium 2-benzo(1,3)dioxol-5-yl-4-(4-methoxy-phenyl)-4-oxo-3-(3,4,5-trimethoxybenzyl)-but-2-enoate) for human endothelin (ET)<sub>A</sub> and ET<sub>B</sub> receptors. In human coronary artery and saphenous vein the affinity of the ET<sub>A</sub> receptor for PD156707 was 0.15 ± 0.06 nM and 0.5 ± 0.13 nM, respectively. Competition experiments in human left ventricle and kidney revealed that PD156707 had 1,000- to 15,000-fold selectivity for the ET<sub>A</sub> receptor over the ET<sub>B</sub> receptor. This selectivity was confirmed autoradiographically. In human coronary artery, mammary artery and saphenous vein PD156707 (3–300 nM) potently antagonized the vasoconstrictor responses to ET-1. The pA<sub>2</sub> values estimated from the Gaddum-Schild equation were 8.07 ± 0.09, 8.45 ± 0.11 and 8.70 ± 0.13, respectively. The concentration-response curves to ET-1 were shifted to the right in parallel fashion, without reduction of the maximum response. However, the regression lines fitted to the resulting Schild data deviated significantly from one. PD156707 appeared to be a more effective antagonist at lower concentrations than at the higher ones. It is possible that PD156707, a sodium salt, was reverting to a less soluble form which results in underestimation of its potency. These data show that PD156707 is a potent and selective antagonist at human ET<sub>A</sub> receptors and will be useful in clarifying the role of the endothelin peptides in human cardiovascular disease.