tbuckley/cabot-search huggingface dataset:
Abstract 2056: Identification of microscopic ovarian tumor foci utilizing a novel imaging device in a murine ovarian cancer model, an opportunity to improve optimal cytoreduction
Authors: Youngjeong Na, Tim Kwok, Christopher Awtrey, David B. Strasfeld, Jorge M. Ferrer, David Lee, Michael J. Birrer
Journal: Cancer Research (2014)

Abstract Survival of ovarian cancer patients is directly related to the amount of residual disease present after debulking surgery. In 80% of the cases, extensive microscopic cancer remains even after the patient is deemed optimally debulked. Hence, detection of sub-mm cancer clusters during tumor excision is a critical unmet need. Recent studies have shown that reducing the dimensions of residual cancer to less than 1 mm significantly improves clinical outcomes. In the present study we evaluate the performance of a fluorescent, molecular imaging agent, LUM015 (Lumicell, Wellesley, MA), which is activated by cathepsin enzymes in the tumor, and a wide-field-of-view imaging device (Lumicell) to detect sub-mm residual cancer clusters in an mouse model for ovarian cancer. In this study, we generated orthotopic ovarian cancer mouse models(n=10) with well characterized serous ovarian cancer cell lines, CP70 and SKOV3. Once the tumor disseminated, the imaging agent LUM015 (3.52 mg/kg) was injected via the tail vein, and after 6 hours, the mice were euthanized. Tumor debulking was performed throughout the abdominal cavity. After debulking, the whole abdominal wall was dissected in 4 quadrants, organs were harvested and all were imaged with the LUM device. Features exhibiting high fluorescence were marked and dissected, prepared into slides, and stained with hematoxylin and eosin for pathologic correlation with LUM015 fluorescence imaging. In 36 tissues from orthotopic ovarian cancer mouse models, LUM015 imaging system could detect the tumor presence with 100% of sensitivity and 60% of specificity. We demonstrated that the imaging system can detect sub-mm cancer clusters that could not be identified with visual inspection. With a cathepsin activated fluorescence imaging molecule (LUM015) and a wide-field-of-view imaging device, we detected microscopic residual ovarian cancer tumors in orthotopic xenograft models after debulking grossly with high sensitivity. Translation of this imaging technology into the clinical setting may help to detect microscopic residual tumor features during the debulking operation in ovarian cancer. Citation Format: Youngjeong Na, Tim Kwok, Christopher Awtrey, David B. Strasfeld, Jorge M. Ferrer, David Lee, Michael J. Birrer. Identification of microscopic ovarian tumor foci utilizing a novel imaging device in a murine ovarian cancer model, an opportunity to improve optimal cytoreduction. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2056. doi:10.1158/1538-7445.AM2014-2056
Review: In type 2 diabetes, GLP-1 agonists plus basal insulin reduce HbA<sub>1c</sub> without increasing hypoglycemia
Authors: Naykky Singh Ospina, Victor M. Montori
Journal: Annals of Internal Medicine (2015)

Source Citation Eng C, Kramer CK, Zinman B, Retnakaran R. Glucagon-like peptide-1 receptor agonist and basal insulin combination treatment for the management of type 2 diabetes: a systematic review and meta-analysis. Lancet. 2014:Sep 11. [Epub ahead of print]. 25220191
Prognostic Value of Epicardial Coronary Artery Constriction to the Cold Pressor Test in Type 2 Diabetic Patients With Angiographically Normal Coronary Arteries and No Other Major Coronary Risk Factors
Authors: Alain Nitenberg, Paul Valensi, Régis Sachs, Emmanuel Cosson, Jean-Raymond Attali, Isabelle Antony
Journal: Diabetes Care (2004)

OBJECTIVE—Endothelium-dependent coronary dilation is impaired in diabetic patients and has been found to independently predict cardiovascular events (CVEs) in patients with multiple coronary risk factors. The aim of this study was to evaluate the outcome of type 2 diabetic patients on the basis of epicardial coronary dysfunction. RESEARCH DESIGN AND METHODS— We examined 56 control subjects (aged 51.7 ± 6.4 years) using coronary artery response to the cold pressor test (quantitative coronary angiography) and compared them with 72 type 2 diabetic patients (aged 50.3 ± 8.5 years) without other major coronary risk factors. RESULTS—Average diameter change was 17.2 ± 10.4% in the control subjects, dilation occurred in 91.1% of subjects, no change occurred in 8.9%, and there was no constriction. Average diameter change was −14.4 ± 12.1% in diabetic patients (P &amp;lt; 0.001 vs. control subjects), constriction occurred in 73.6%, no change occurred in 26.4%, and there was no dilation. CVEs were recorded with a mean follow-up of 45 ± 19 months. There was 1 CVE in the control group and 26 CVEs in 18 of 72 diabetic patients (P &amp;lt; 0.001 vs. control subjects), with 23 events in 16 of 53 diabetic patients with coronary artery constriction (P &amp;lt; 0.001 vs. control subjects), and 3 events in 2 of 19 diabetic patients with no diameter change (NS vs. control subjects). CONCLUSIONS—In type 2 diabetic patients without other major coronary risk factors, constriction of angiographically normal coronary arteries to the cold pressor test is predictive of long-term CVEs.
Abstract 508: Synergistic effect of β-catenin knockdown and radiation combination toward AMC-HN9 head and neck cancer cells is associated with down-regulation of Ku expression.
Authors: Hyo Won Chang, Hyo Jung Kim, So Young Moon, Mi Ra Kim, Seong Who Kim, Sang Yoon Kim
Journal: Cancer Research (2010)

Abstract Based on our previous report showing that up-regulation of Ku70/Ku80 in AMC-HN9 (HN9) cells is required for cell-cycle re-entry and cell proliferation following irradiation, further study to investigate the mechanism by which β-catenin knockdown sensitizes the cells to irradiation have been conducted. β-Catenin, as a multifunctional protein involved in cadherin-mediated cell-cell adhesion and Wnt signaling transduction, have been well known to regulates cell growth and survival following radiation in various types of cancer cells. However, the molecular mechanisms by which β-catenin affects radiation sensitivity are still not fully understood, which led us to explore, with regard to regulation of Ku70/Ku80 expression, the molecular mechanism of the synergistic effects of β-catenin silencing and radiation combination in radio-resistant head and neck cancer cells. β-Catenin silencing using small interfering RNA(siRNA) down-regulated β-catenin expression up to 72 h both in membranous and nuclear fraction of HN9 cells. β-Catenin knockdown induced cell cycle arrest on G1 phase and thus decreased cell proliferation, but didn't cause any DNA damage response and cell death. Whereas expression of Ku70/Ku80 was up-regulated in HN9 cell following irradiation (4Gy), in the cells treated with combination of radiation and β-catenin siRNA, Ku70/Ku80 expression were dramatically decreased. In addition, when β-catenin silenced cells were exposed to single dose of radiation, irradiation-induced cell death was much more increased from 3.1% to 13% and accompanied with caspase-3 activation and PARP cleavage. Taken together, these results suggest that inhibition of Ku expression through β-catenin silencing is associated with its radio-sentitizing effect in HN9 cells and further studies to clarify the pathway linking β-catenin signaling and regulation of Ku70/Ku80 expression are warranted. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 508.
Immunohistochemical localization of transforming growth factor-beta 1 in the nonnecrotizing granulomas of pulmonary sarcoidosis.
Authors: A H Limper, T V Colby, M S Sanders, S Asakura, P C Roche, R A DeRemee
Journal: American Journal of Respiratory and Critical Care Medicine (1994)

Sarcoidosis is a chronic inflammatory disease of unknown cause characterized by the formation of nonnecrotizing granulomas in affected tissues, most notably the lungs. Granuloma healing may result in pulmonary fibrosis and respiratory impairment in some patients. Transforming growth factor-beta 1 (TGF-beta 1) is a potent cytokine that promotes fibrosis by enhancing the synthesis of extracellular matrix components, including fibronectin and the alpha 5 beta 1 fibronectin receptor. The role of TGF-beta 1 in promoting lung fibrosis in the setting of pulmonary sarcoidosis has not yet been investigated. Accordingly, we determined the extent and distribution of TGF-beta 1 in lung tissue obtained from seven patients with clinical and histologic features of pulmonary sarcoidosis. The tissue distributions of TGF-beta 1, the TGF-beta 1 binding proteoglycan decorin, fibronectin, and the alpha 5 beta 1 fibronectin receptor were assessed immunohistochemically. In all cases, the epithelioid histiocytes comprising nonnecrotizing granulomas of pulmonary sarcoidosis contained abundant TGF-beta 1. We further demonstrated decorin, fibronectin, and the alpha 5 beta 1 fibronectin receptor within nonnecrotizing granulomas and in the fibrous tissue surrounding the lesions. TGF-beta 1 staining was also observed in bronchiolar epithelial cells, hyperplastic Type II pneumocytes, and occasional alveolar macrophages. This study demonstrates enhanced tissue localization of TGF-beta 1 and related extracellular matrix proteins associated with the nonnecrotizing granulomas of pulmonary sarcoidosis. Through its actions on matrix protein synthesis, TGF-beta 1 may modulate the fibrotic repair process accompanying granuloma healing in sarcoidosis.
Abstract 944: The effect of endogenous peroxidase activity on cell culture-based drug activity screening.
Authors: Joshua Bakhsheshian, Alexandra D.T. Kwit, Kyle R. Brimacombe, Matthew D. Hall, Michael M. Gottesman
Journal: Cancer Research (2013)

Abstract Reactive oxygen species (ROS) are known to play an intrinsic role in mediating the cancer cell killing of many commonly employed anticancer agents. In many cases this is due to direct production of ROS via interaction with cellular components. This evidence is based partly on observations that ROS-scavenging agents such as N-acetylcysteine or catalase reverse killing in cancer cells. Catalase enzymatically degrades hydrogen peroxide (H2O2) into dioxygen and water. We examined the effect of catalase (5,000 U/mL) supplemented in growth media on the efficacy of ∼800 cytotoxic small molecules. Strong reversal of toxicity was found for 34 compounds with diverse mechanisms, including tubilin-interacting drugs. This implies that cytotoxins may have diminished efficacy in vivo due to the high activity of catalase in blood (∼100,000 U/mL). Surprisingly, we found that assays conducted with supplementation of catalase in media at levels greater than &amp;gt;20,000 U/mL, intended to replicate the endogenous environment, were highly toxic to cells. This phenomenon has been replicated in a number of cell lines from various tissue origins. One implication of this funding is that cancer cell lines require baseline ROS levels for cell survival proliferation, consistent with the cancer ROS addiction theory. Given that cell culture medium lacks peroxidase activity we conclude that the precise cell culture environment is a critical determinant of the results obtained when screening drugs for toxicity to cancer cells. Citation Format: Joshua Bakhsheshian, Alexandra D.T. Kwit, Kyle R. Brimacombe, Matthew D. Hall, Michael M. Gottesman. The effect of endogenous peroxidase activity on cell culture-based drug activity screening. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 944. doi:10.1158/1538-7445.AM2013-944
Serum macrophage colony-stimulating factor (M-CSF) level is elevated in patients with old cerebral infarction related to vascular damage
Authors: Akira Suehiro, Hiroshi Tsujioka, Hiroshi Yoshimoto, Satoshi Higasa, Eizo Kakishita
Journal: American Journal of Hematology (1999)

We measured the serum levels of macrophage colony-stimulating factor (M-CSF) in 37 patients with an old cerebral infarction who had been surmised to have a damaged vessel wall and who had been in a stable condition for over three months after stroke onset, and those of 41 healthy control subjects. The M-CSF levels in the patients were significantly higher (P < 0.01) than those of the controls at 1320.4 +/- 410.6 unit/ml and 853.9 +/- 180.3 unit/ml, respectively. The plasma levels of von Willebrand factor (vWF) antigen (P < 0.01) and thrombomodulin (TM) (P < 0.05), as well as those of thrombin-antithrombin III (TAT) complex (P < 0.05), prothrombin fragment 1+2 (F1+2) (P < 0.02), D-dimer products of crosslinked fibrin degradation products (D-dimer) (P < 0.01), and plasmin-antiplasmin (PAP) complex (P < 0.05) in the patients were also significantly higher than those in the controls. Significant positive correlations (P < 0.01) were found between these parameters and the M-CSF level, but there was no significant correlation between the M-CSF level and the white blood cell count, serum lipids, or blood pressure. Based on these results, we suggest that an increased M-CSF level indicates vascular damage or a thrombotic state in patients with an old cerebral infarction.
Rapid Detection of Cytomegalovirus Pulmonary Infection by Bronchoalveolar Lavage and Centrifugation Culture
Authors: STEPHEN W. CRAWFORD
Journal: Annals of Internal Medicine (1988)

Cytomegalovirus infection remains a major cause of morbidity and mortality in marrow transplant recipients. Results of a rapid centrifugation viral culture of bronchoalveolar lavage specimens from 33 marrow transplant recipients with pneumonia were compared with those for conventional viral culture of concurrently or subsequently obtained lung tissue. The centrifugation culture results were also compared to results of cytologic and immunochemical examination of these specimens. Centrifugation culture was positive within 16 hours of inoculation in 22 of 23 (96%) specimens from patients with positive conventional culture of lung tissue. Detection of cells positive for cytomegalovirus by immunofluorescent antibody staining or cytologic identification was less sensitive (59% and 29%, respectively). There was no evidence of cytomegalovirus in specimens from patients without evidence of cytomegalovirus pulmonary infection by any technique. The sensitivity (96%) and specificity (100%) of centrifugation culture of specimens from marrow transplant recipients approach that of viral culture of lung tissue.
Pulmonary Hypersensitivity Transfusion Reactions.
Authors: Howard N. Ward
Journal: Annals of Internal Medicine (1970)

Meeting Abstracts1 May 1970Pulmonary Hypersensitivity Transfusion Reactions.Howard N. Ward, M.D.Howard N. Ward, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-72-5-796_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptDuring the past 2 years four patients with "non-cardiac pulmonary edema" that occurred as a complication of blood transfusion have been observed. In this report the clinical features of the syndrome are described, and data are presented that suggest that the pulmonary infiltrates represent a hypersensitivity type of pneumonitis possibly secondary to leukoagglutinins in either donor or recipient blood. Typical reactions were characterized by abrupt onset of chills, fever, tachycardia, a non-productive cough, dyspnea, and in some, hypotension and urticaria. The acute symptoms lasted only several hours; fever and roentgenographically visible pulmonary infiltrates persisted up to 48 hr. A postreaction... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: Howard N. Ward, M.D.Affiliations: St. Louis, Mo. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 May 1970Volume 72, Issue 5Page: 796-796KeywordsBlood donorsBlood transfusionDyspneaEdemaFeversHypersensitivityHypotensionPneumonitisTachycardiaUrticaria ePublished: 1 December 2008 Issue Published: 1 May 1970 PDF downloadLoading ...
Adverse Fetal Outcome Following Maternal Rubella After the First Trimes ter of Pregnancy
Authors: Janet B. Hardy, George H. McCracken, Mary Ruth Gilkeson, John L. Sever
Journal: JAMA (1969)

Of the 24 women with clinical and laboratory evidence of rubella between the 14th and 31st weeks of pregnancy, two experienced fetal loss and 22 were delivered of liveborn infants who survived. Rubella virus was recovered from the products of conception in the instances of fetal loss, and from the throats of two and the placenta of another of the 19 live-born tested. Seven of the live-born children are normal, one had an elevated serum IgM level at birth, and none of the seven have detectable rubella antibody after six months of age. Fifteen of the live-born children are suspected to be abnormal; ten of these have problems in communication. Of the 15, six have had elevated serum IgM levels and ten have had detectable levels of rubella antibody after 6 months of age.
Abstract P6-07-04: Evaluation of Adjuvant! Online for primary operable grade 2 breast cancers with 10-year follow-up
Authors: B Van Calster, O Brouckaert, H Wildiers, M-R Christiaens, C Weltens, R Paridaens, E Van Limbergen, P Neven
Journal: Cancer Research (2012)

Abstract Background: In primary operable breast cancers, Adjuvant!Online (AOL) uses demographics and clinic-pathological markers to predict the 10-year breast cancer specific survival (BCSS) with and without adjuvant systemic therapy. Grade 2 BC is a difficult group for adjuvant treatment decisions. We compared the observed with the AOL-estimated BCSS rate in our centre in consecutive women primary operated for a grade 2 BC. Patients and Methods: All patients with grade 2 primary operable breast cancer from UZL diagnosed between 1/1/2000 and 31/3/2002. After 10 years of follow-up, patients were evaluated for BCSS and dead from other cause. For each patient, the adjuvant! Online algorithm (version 8) was applied to predict BCSS taking the patients' adjuvant therapy into account. Adjuvant! Online was validated by assessing the calibration and discrimination. Discrimination was assessed by the area under the ROC curve. The expected BCSS rate was obtained by averaging the predicted probabilities of BCSS. Next, logistic calibration was performed by predicting observed BCSS within 10 years of follow-up with the odds of the predicted probability of BCSS (odds BCSS). In this analysis, the intercept should be 0 and the coefficient for odds BCSS 1 for perfect calibration of the predicted probabilities. A likelihood ratio test is used to test the null hypothesis of perfect calibration. Results: Six patients were lost to follow-up. The median age at diagnosis of the remaining 445 patients was 56 years (IQR 48 to 67 years, range 26 to 89 years). In 433/445 cases (97%), the prediction from AOL was available. The median AOL predicted probability for BCSS was 89.1% (IQR 83.2 to 94.6%, range 20.3 to 98.9%). The observed BCSS rate was 93.3% whereas the AOL-expected rate was 86.7%. The likelihood ratio test for perfect calibration was statistically significant (p &amp;lt; 0.0001), with an intercept of −1.55 (95% CI −2.33 to −0.84) and a coefficient for odds BCSS of 0.55 (95% CI 0.19 to 0.92). AOL strongly underestimated the probability of BCSS. The AUC was 0.65 (95% CI 0.53 to 0.76). For patients that did not die from BC within 10 years, the median estimated BCSS probability by AOL was 89.6% (IQR 83.5 to 94.6 %), whereas for patients that died from BC the median estimated probability was 83.9 % (IQR 77.9 to 94.5%). Conclusion: Although Adjuvant! Online achieved moderate discrimination between those that did and those that did not die from BC within 10 years FU, it clearly underestimated the probability of BCSS in our sample. Citation Information: Cancer Res 2012;72(24 Suppl):Abstract nr P6-07-04.
A New Anthelmintic
Authors: John D. Archer
Journal: JAMA (1974)

Among the intestinal parasitic infestations extant in this country, whipworm disease (trichuriasis) has been one especially difficult to manage. Treatment failures have been common with available drugs. Although dithiazanine iodide (Delvex) apparently was quite effective, it produced some unfortunate toxic effects and is no longer marketed.<sup>1</sup> A notable advance in the treatment of whipworm infestations appears imminent. In this issue ofThe Journal(p 1408, p 1412), Miller et al and Wolfe and Wershing report quite gratifying results with a new drug, mebendazole, in two series of patients with the disease. High reductions in egg counts occurred, and while cures were not uniformly achieved, adequate dosage usually produced them. For inexplicable reasons, infestations that were not eradicated in the Miller series were usually light ones. In the Wolfe and Wershing series, however, from a different geographical area, the cure rate was lower with massive infestations. Contrary to an earlier
Effects of selectively altering collateral driving pressure on regional perfusion and function in occluded coronary bed in the dog.
Authors: S Kaul, N G Pandian, J L Guerrero, L D Gillam, R D Okada, A E Weyman
Journal: Circulation Research (1987)

To determine whether selectively altering the coronary perfusion pressure in the adjacent nonoccluded vessel has any influence on the occluded bed, the effects of alterations in the perfusion pressure of the left anterior descending coronary artery on the perfusion and function of the acutely occluded left circumflex coronary (LC) arterial bed were studied in 10 anesthetized open-chest dogs. Radiolabelled microsphere-assessed regional myocardial perfusion and endocardial excursion determined by two-dimensional echocardiography were measured during control conditions prior to mid-LC occlusion with left anterior descending coronary arterial pressure (LADP) equal to aortic pressure (AoP) (Stage 0) and to 3 randomly performed postocclusion stages. At each postocclusion stage, the perfusion territory of the occluded LC bed (area at risk) was measured in vivo using myocardial contrast two-dimensional echocardiography. During Stage 1 (LADP = AoP), area at risk was 5.1 +/- 0.9 cm2 (x +/- 1 SD) and transmural blood flow to the LC arterial bed decreased from 0.96 +/- 0.50 ml/min/g (Stage 0) to 0.16 +/- 0.12 ml/min/g (p less than 0.01), while endocardial excursion decreased from 28.0 +/- 9.0% to 2.0 +/- 10.0% (p less than 0.01). During Stage 2 (LADP greater than AoP), area at risk decreased to 4.4 +/- 1.0 cm2 compared with Stage 1 (p less than 0.01), and transmural blood flow, endocardial:epicardial blood flow ratio, and endocardial excursion increased to 0.51 +/- 0.39 ml/min/g, 0.64 +/- 0.20, and 14 +/- 6%, respectively (p less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
Effect of icodextrin on volume status, blood pressure and echocardiographic parameters: A randomized study
Authors: Constantijn J.A.M. Konings, Jeroen P. Kooman, Marc Schonck, Ulrich Gladziwa, Joris Wirtz, A. Warmold van den Wall Bake, Paul G. Gerlag, Steven J. Hoorntje, Johannes Wolters, Frank M. Van Der Sande, Karel M.L. Leunissen
Journal: Kidney International (2003)