tbuckley/cabot-search huggingface dataset:
Abstract 4332: Diagnostic applications of fatty acid synthase monoclonal antibodies
Authors: Walter P. Carney, Wendy Zhang, David Jarosz, Patrick Muraca, Sunny TAM
Journal: Cancer Research (2014)

Abstract Fatty acid synthase (FASN), is an enzyme capable of de novo fatty acid synthesis and highly expressed and activated in most human carcinomas. Fatty acid synthase is a multi-enzyme protein that catalyzes fatty acid synthesis and is not a single enzyme but a whole enzymatic system composed of two identical 272 KDa multifunctional polypeptides. Fatty-acid synthesis is now associated with clinically aggressive tumor behavior, tumor-cell growth and is associated with poor prognosis in prostate and breast cancer. Its inhibition is selectively cytotoxic to human cancer cells. Thus, FASN and fatty acid metabolism have become an important focus for the diagnostic and treatment of cancer. We have developed a panel of anti-human FASN Mab for application to several diagnostic platforms. Recombinant full length intact FASN protein (rFASN) was used to immunized C57BL/6 mice, sera were collected from pre- and post-immunized mice and tested by ELISA and Western blot on rFASN protein or rFASN immobilized on Western blot. Spleens from mice with the highest anti-FASN antibody titer was fused with a mouse myeloma cell line Sp2/0-Ag14 for hybridoma production. Among the panel of positive anti-FASN clones selected, two clones with isotype of IgG1kappa have shown strong reactivity to the rFASN and native FASN protein on Western analysis. Upon further Western analyses with cell lysates, we have demonstrated specific detection of FASN protein in human embryonic kidney cells (HEK), HEK cells overexpressing the rFASN protein, HOP-62 cells which express low levels of FASN and MALME cells, which express high levels of FASN. Additional biochemical characterization of these antibodies is ongoing, and includes epitope mapping and reactivity to various FASN protein fragments. These anti-FASN antibodies will be tested by IHC, ELISA to compare tissues expression levels of FAS by IHC or circulating levels of the FASN in both normal and cancer patients. With the increasing development of FAS inhibitors these antibodies will be tested as Companion Diagnostics and tested to see if the rise and fall of the FASN circulating levels correspond with cancer progression or therapy response. Citation Format: Walter P. Carney, Wendy Zhang, David Jarosz, Patrick Muraca, Sunny TAM. Diagnostic applications of fatty acid synthase monoclonal antibodies. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4332. doi:10.1158/1538-7445.AM2014-4332
Evaluation of a multicentre study of synchronous application of narrowband ultraviolet B phototherapy (TL-01) and bathing in Dead Sea salt solution for psoriasis vulgaris
Authors: R. Schiffner, J. Schiffner-Rohe, G. Wölfl, M. Landthaler, A. Gläßl, TH. Walther, F. Hofstädter, W. Stolz
Journal: British Journal of Dermatology (2000)

Journal Article Evaluation of a multicentre study of synchronous application of narrowband ultraviolet B phototherapy (TL‐01) and bathing in Dead Sea salt solution for psoriasis vulgaris Get access R. Schiffner, R. Schiffner Department of Dermatology, University of Regensburg, Germany Search for other works by this author on: Oxford Academic Google Scholar J. Schiffner‐Rohe, J. Schiffner‐Rohe Department of Dermatology, University of Regensburg, Germany Search for other works by this author on: Oxford Academic Google Scholar G. Wölfl, G. Wölfl Cancer Registry Center Regensburg at the University of Regensburg, Germany Search for other works by this author on: Oxford Academic Google Scholar M. Landthaler, M. Landthaler Department of Dermatology, University of Regensburg, Germany Search for other works by this author on: Oxford Academic Google Scholar A. Gläßl, A. Gläßl Department of Dermatology, University of Regensburg, Germany Search for other works by this author on: Oxford Academic Google Scholar TH. Walther, TH. Walther Department of Dermatology, University of Regensburg, Germany Search for other works by this author on: Oxford Academic Google Scholar F. Hofstädter, F. Hofstädter Institute of Pathology, University of Regensburg, 93042 Regensburg, Germany Search for other works by this author on: Oxford Academic Google Scholar W. Stolz W. Stolz Department of Dermatology, University of Regensburg, Germany Professor Wilhelm Stolz, M.D.E‐mail: wilhelm.stolz@klinik.uni‐regensburg.de Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 142, Issue 4, 1 April 2000, Pages 740–747, https://doi.org/10.1046/j.1365-2133.2000.03420.x Published: 01 April 2000
Uncoupling proteins: the unravelling of obesity?
Authors: A. Berger, M. Brand, S. O'Rahilly
Journal: BMJ (1998)

Obesity is becoming a worldwide epidemic, with one in three people in the United States now obese and an increasing prevalence of obesity in developing countries. The discovery of the hormone leptin in 19941 was critical to our understanding of some of the mechanisms of obesity, and an explosion of research into the molecules involved in the normal control of appetite and energy expenditure has resulted. Once these are better defined potential genetic and pharmacological approaches to treating obesity will be opened up. Of particular interest are leptin itself (and how this circulating hormone may regulate appetite via receptors in the hypothalamus) and uncoupling proteins, which are molecules outside the brain that may influence energy expenditure.

Leptin's existence—and the demonstration that leptin deficient mice lose weight if they are given the hormone—represents an intellectual milestone. Obesity should no longer be thought of as a lack of will power: body weight is subject to physiological control. In Cambridge three morbidly obese children have recently been identified as having almost total leptin deficiency.2 One child has been receiving leptin supplements, and the results …
Toward a more accurate estimate of the prevalence of hepatitis C in the United States
Authors: Brian R. Edlin, Benjamin J. Eckhardt, Marla A. Shu, Scott D. Holmberg, Tracy Swan
Journal: Hepatology (2015)

Data from the 2003-2010 National Health and Nutrition Examination Survey (NHANES) indicate that about 3.6 million people in the United States have antibodies to the hepatitis C virus, of whom 2.7 million are currently infected. NHANES, however, excludes several high-risk populations from its sampling frame, including people who are incarcerated, homeless, or hospitalized; nursing home residents; active-duty military personnel; and people living on Indian reservations. We undertook a systematic review of peer-reviewed literature and sought out unpublished presentations and data to estimate the prevalence of hepatitis C in these excluded populations and in turn improve the estimate of the number of people with hepatitis C in the United States. The available data do not support a precise result, but we estimated that 1.0 million (range 0.4 million-1.8 million) persons excluded from the NHANES sampling frame have hepatitis C virus antibody, including 500,000 incarcerated people, 220,000 homeless people, 120,000 people living on Indian reservations, and 75,000 people in hospitals. Most are men. An estimated 0.8 million (range 0.3 million-1.5 million) are currently infected. Several additional sources of underestimation, including nonresponse bias and the underrepresentation of other groups at increased risk of hepatitis C that are not excluded from the NHANES sampling frame, were not addressed in this study.The number of US residents who have been infected with hepatitis C is unknown but is probably at least 4.6 million (range 3.4 million-6.0 million), and of these, at least 3.5 million (range 2.5 million-4.7 million) are currently infected; additional sources of potential underestimation suggest that the true prevalence could well be higher.
Abstract P040: HEALS Lifestyle intervention through African American churches to control hypertension
Authors: Sunita Dodani, Sahel Arora, Carmen Smotherman, Dale Kraemer
Journal: Circulation (2014)

Objective: African Americans (AAs) living in the “Stroke Belt” (Southeastern US including Northeast Florida) are at elevated risk for cardiovascular diseases (CVDs). An approach of proven efficacy is to target hypertension (HTN) as the major modifiable risk factor for CVDs, and to do so by evidence-based lifestyle changes. Here we present the findings of faith-based socio-culturally tailored HEALS HTN control program (modified from PREMIER and DASH diets), offered to AA church parishioners living in the food dessert areas of Duval County, Florida. Methods: Large urban church (membership &gt; 400) volunteered for 9-month HEALS program. Four church members were trained as program leaders called CHAs-Church health Advisors, who after 10 hours of receiving training on HEALS session from PREMIER experts delivered weekly HEALS sessions to high-risk church members. 35 church members who were 25-75 year old, self-described AAs and HTN - as defined by JNC-7 were recruited. Farmer Market trucks from Clara White Mission supplied fresh fruits and vegetables (FFVs) at the church. Outcome measures included change in blood pressure (BP), weight and change in consumption of FFVs (measured by increases in purchases). Results: The study is ongoing. At baseline, 22% members were pre-HTN, 50% had Stage 1 HTN, and 22% with Stage 2 HTN. After receiving 12 wks HEALS program, mean reduction in systolic and diastolic BP were -14.6 mmHg (±14.3, p= 0.0002) and -6 mmHg (± 9.6, p=0.019) respectively. Mean weight reduction was -3.38 Kg (± 2.23, p&lt;0.0001). For the first two months, the total FFVs sales increased from $97.50 in the first month to $117.50 in the second month. Majority of the sale was on fruits such as watermelons (60%). Conclusion: The translation and sustainability of evidence-based lifestyle modification in community-based settings is possible, particularly within churches, which represent the most influential institution in the community lives of AA
De-Escalation of the Alemtuzumab Dose Prior to Nonmyeloablative HLA-Identical Sibling Transplantation: Crucial Role of Timing.
Authors: Ronjon Chakraverty, Maria Dimopoulou, Julie Glanville, Kirsty Thomson, Karl Peggs, Donald Milligan, Gordon Cook, Graham Jackson, Anne Parker, Ann Hunter, Nigel Russell, Rachel Pearce, Stephen Mackinnon
Journal: Blood (2006)
The effect of positive expiratory pressure for airway clearance on ventilation inhomogeneity in individuals with stable COPD and chronic sputum expectoration
Authors: Christian Osadnik, Christopher Stuart-Andrews, Samantha Ellis, Bruce R. Thompson, Christine F. McDonald, Anne E. Holland
Journal: European Respiratory Journal (2013)

Background: Positive expiratory pressure (PEP) is commonly used to promote airway clearance, however little is known about its mechanism of action in individuals with COPD.

Aim: To determine whether PEP mask therapy improves ventilation inhomogeneity and increases functional residual capacity (FRC) more than controlled huffing and coughing.

Methods: Twelve participants with stable COPD (mean FEV1 45% predicted) and chronic sputum expectoration performed PEP mask therapy (10-20 cms H2O) or controlled huffing and coughing in random order on separate days. Measures of acinar and conductive airways ventilation (Sacin, Scond), lung volumes, spirometry and sputum weight were recorded before, immediately after and 90 minutes following treatment. Ease of expectoration (visual analogue scale [VAS], higher = worse) and oxyhaemoglobin saturation were assessed immediately following treatment. Data were analysed via linear mixed model analyses.

Results: No significant difference existed between the two test conditions for any test parameter at any time point. Immediately following the PEP and control conditions, mean Sacin was 0.465L-1 and 0.438L-1, respectively (p = 0.45 for comparison between conditions) and Scond 0.042L-1 and 0.039L-1 (p = 0.55). Mean FRC was 5.07L and 5.03L (p = 0.38), mean sputum wet-weight was 7.06g and 6.15g (p = 0.51), and VAS scores were 4.8cm and 4.1cm (p = 0.53), respectively.

Conclusion: Any therapeutic benefits of PEP mask therapy in individuals with COPD are unlikely to be mediated by improvements in ventilation inhomogeneity or increases in lung volumes.
Paternalism or partnership?
Authors: A. Coulter
Journal: BMJ (1999)

<h3>Abstract</h3> Homo-oligomeric ligand-activated proteins are ubiquitous in biology. The functions of such molecules are commonly regulated by allosteric coupling between ligand binding sites. Understanding the basis for this regulation requires both quantifying the free energy ΔG transduced between sites, and the structural basis by which it is transduced. We consider allostery in three variants of the model ring-shaped homo-oligomeric <i>trp</i>RNA binding attenuation protein, TRAP. First, we developed nearest-neighbor statistical thermodynamic binding models comprising microscopic free energies for ligand binding to isolated sites ΔG<sub><b>N0</b></sub>, and for coupling between one or both adjacent sites, ΔG<sub>N1</sub> and ΔG<sub>N2</sub>. Using the resulting partition function (PF) we explored the effects of these parameters on simulated population distributions for the 2<sup>N</sup> possible liganded states. We then experimentally monitored liganddependent population shifts using conventional spectroscopic and calorimetric methods, and using native mass spectrometry (MS). By resolving species with differing numbers of bound ligands by their mass, native MS revealed striking differences in their ligand-dependent population shifts. Fitting the populations to a binding polynomial derived from the PF yielded coupling free energy terms corresponding to orders of magnitude differences in cooperativity. Uniquely, this approach predicts <i>which</i> of the possible 2<sup>N</sup> liganded states are populated at different ligand concentrations, providing necessary insights into regulation. The combination of statistical thermodynamic modeling with native MS may provide the thermodynamic foundation for a meaningful understanding of the structure-thermodynamic linkage that drives cooperativity. <h3>TOC Figure (draft)</h3>
Physicians as Citizens—Reply
Authors: Russell L. Gruen, Steven D. Pearson, Troyen A. Brennan
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Enoxaparin vs Unfractionated Heparin in Acute Coronary Syndrome—Reply
Authors: Michael A. Blazing
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Mumps outbreak - New York, New Jersey, Quebec, 2009.
Authors: P. High, E. F. Handschur, O. S. Eze, B. Montana, C. Robertson, C. Tan, J. B. Rosen, K. P. Cummings, M. K. Doll, J. R. Zucker, C. M. Zimmerman, T. Dolinsky, S. Goodell, C. Schulte, D. Blog, M. A. Leblanc, Y. A. Li, A. Barskey, G. Wallace, K. Gallagher, G. Armstrong, L. Lowe, R. McNall, J. Rota, P. Rota, C. Hickman
Journal: MMWR Morbidity and Mortality Weekly Report (2009)
Abstract 16886: LDL4- a Novel Predictor of Coronary Artery Disease Severity
Authors: Kevin P Bliden, Udaya S Tantry, Shachi Pandya, Rahil Rafeedheen, Peter P Toth, Ali Tabrichzi, Charles Cummings, Christopher Franzese, Martin Gesheff, Paul A Gurbel
Journal: Circulation (2014)

Background: Hypercholesterolemia is a well-established risk factor for coronary artery disease (CAD). There is a growing focus on preventive cardiology through identification of patients at high risk for CAD. CAD is frequently noted in patients with elevated cholesterol levels but patients with normal levels of total cholesterol and LDL-C are also noted to have CAD including severe CAD. Small-dense LDL particle subtypes (LDL3 and LDL4) have been found to be more oxidizable and atherogenic. We aim to identify if cholesterol particle subtypes are more predictive of CAD than standard lipid testing. Methods: Blood samples were obtained immediately prior to cardiac catheterization in patients with suspected CAD. Detailed lipid profiling was performed using VAP cholesterol test and AtherOx testing was performed using immunoassay in patients not on lipid lowering therapy. 138 patients had CAD severity categorized angiographically as none/minor CAD (less than 20% luminal diameter stenosis [LDS]), and severe CAD (...
Characteristics of alcoholics who attempt suicide
Authors: A Roy, D Lamparski, J DeJong, V Moore, M Linnoila
Journal: American Journal of Psychiatry (1990)

Of 298 alcoholic subjects studied by the authors, 19% (N = 57) had attempted suicide. Compared with nonattempters, suicide attempters were significantly more likely to be female, to have a lower socioeconomic status, to be younger, to have begun heavy drinking and experienced the onset of alcohol-related problems at an earlier age, to consume a greater amount of alcohol when drinking, and to have additional lifetime psychiatric diagnoses of major depression, antisocial personality disorder, substance abuse, panic disorder, phobic disorder, or generalized anxiety disorder. Also, significantly more attempters had first- or second-degree relatives who abused alcohol.
Differentiation of normal human keratinocytes influences hexavalent chromium uptake and distribution and the ability of cells to withstand Cr(VI) cytotoxicity
Authors: None Henshaw, None Morris, None Mac Neil
Journal: British Journal of Dermatology (1999)

The degree of differentiation of normal human keratinocytes determines the biology of the cells to a large extent. We have previously documented that keratinocytes from different donors differ significantly in their ability to withstand hexavalent chromium [Cr(VI)]‐induced cytotoxicity. Several factors may contribute to this differing donor sensitivity to Cr(VI). The aims of this study were to investigate to what extent keratinocyte differentiation might influence Cr(VI) uptake and the ability of cells to withstand Cr(VI)‐induced cytotoxicity. Keratinocytes from different donors were cultured under identical conditions and exposed to Cr(VI) (as potassium dichromate) at different points during their maturation process. The degree of differentiation of the cells was assessed using a quantitative assay for involucrin and related to the Cr(VI) cytotoxicity experienced by the cells. Chromium content was measured in whole cell, cytosolic and particulate fractions. While proliferative keratinocytes exposed to Cr(VI) showed a high degree of cytotoxicity to dichromate exposure, the more differentiated cells showed significantly less cytotoxicity but a higher uptake of the metal ion into the cells. The relative percentage of cytosolic chromium was high in the proliferative cells and decreased as the cells matured, suggesting that differentiated cultures were binding most of the chromium to the particulate fraction. Total chromium also increased during differentiation. The use of the channel‐blocking agent 4,4′‐diisothiocyanate‐2‐2′‐stilbenedisulphonic acid confirmed the spatial differences of chromium accumulation in the phenotypically different cultures, in that it prevented Cr(VI) entry into the proliferative cells and attenuated dichromate cytotoxicity in these cultures, but had no effect on the Cr(VI) uptake in differentiated cells, nor did it reduce its cytotoxicity. These data support the hypothesis that the upper differentiated layers of the epidermis are able to offer considerable physical protection to the lower proliferative layers from chemical pro‐oxidants.
Costs and benefits of a one stop clinic compared with a dedicated breast clinic: randomised controlled trial * Commentary: one stop clinics should not be abandoned
Authors: P. Dey
Journal: BMJ (2002)

To determine the cost to the NHS and the impact on anxiety of a one stop clinic for assessing women with suspected breast cancer.Randomised controlled trial.Women aged 35 or over referred with a breast lump.Teaching hospital, north west England.Women were randomly allocated to attend a one stop clinic or a dedicated breast clinic.Reduction in mean anxiety from baseline at 24 hours after the first visit and at 3 weeks and 3 months after diagnosis; mean cost per patient.670 women were randomised. Compared with women who attended the dedicated clinic, patients attending the one stop clinic were less anxious 24 hours after the visit (adjusted mean change in state anxiety _5.7 (95% confidence interval _8.4 to _3.0)) but not at 3 weeks or 3 months after diagnosis. The additional cost to the NHS of a one stop attendance was pound 32 per woman; this was largely explained by greater cytopathological and radiological staff costs.One stop clinics may not be justified in terms of a reduction in short term anxiety.