tbuckley/cabot-search huggingface dataset:
RESISTANCE OF THE MOUSE'S INTESTINAL TRACT TO EXPERIMENTAL SALMONELLA INFECTION
Authors: Marjorie Bohnhoff, C. Phillip Miller, William R. Martin
Journal: The Journal of Experimental Medicine (1964)

Determinations of pH, Eh, and concentrations of acetic, butyric and lactic acids were made on the content of cecum and transverse colon of groups of mice killed 1, 3, and 5 days after oral administration of 50 mg streptomycin. Control observations on untreated mice are reported in the preceding communication. Heat-killed supenatants of suspensions of bowel content were tested in vitro for their ability to inhibit multiplication of our standard streptomycin-resistant strain of Salmonella enteritidis during aerobic and anaerobic incubation. Also tested in like fashion were series of cultures in broth buffered at various pH levels and containing acetic, butyric, and lactic acids in varying concentrations. In colon content of mice on the 1st day after streptomycin treatment, the pH had risen and the concentrations of the fatty acids fallen, a combination of effects which adequately accounts for its inability to inhibit multiplication of Salmonella in vitro and in vivo. By the 3rd day after streptomycin treatment, pH and fatty acid concentrations had returned to normal levels. The susceptibility of mice to oral challenge on the 3rd day was explained by the finding that lactic acid had accumulated in colon content to levels which, in broth, effectively counteracted the activity of inhibitory concentrations of the fatty acids. Other cocarboxylic acids also antagonized the inhibitory activity of the fatty acids; glucose did not.
Multigene/Multisubtype HIV-1 Vaccine Induces Potent Cellular and Humoral Immune Responses by Needle-Free Intradermal Delivery
Authors: Andreas Bråve, Karl Ljungberg, Andreas Boberg, Erik Rollman, Maria Isaguliants, Björn Lundgren, Pontus Blomberg, Jorma Hinkula, Britta Wahren
Journal: Molecular Therapy (2005)

Gene vaccination encounters problems different from those of gene therapy since both a short half-life of the gene and a strong immune response to the gene product are desirable. We have evaluated a DNA vaccine consisting of seven plasmids encoding nine HIV-1 proteins. Using a needle-free delivery device, the Biojector, together with recombinant mouse GM-CSF, this vaccine induced strong gp160 Env- and p24 Gag-specific cellular and humoral immune responses in mice. The rGM-CSF was crucial for inducing both antibodies and antigen-specific CD8(+) T cell responses against both gp160 and p24. A GMP-produced lot of this vaccine, intended for human use, was delivered intradermally or intramuscularly into BALB/c mice at a GLP-accredited animal facility. This vaccine induced strong cellular responses independent of the route of immunization; moreover, no signs of toxicity were detected after histopathological examination of various tissues. Overall, the results indicate that the intradermal delivery of multigene/multisubtype HIV DNA in combination with recombinant GM-CSF is a safe and efficacious strategy for inducing high levels of specific CD8(+) T cells and unusually high titers of antibodies. This vaccine has been approved by the Swedish Medicinal Products Agency and is currently in a Phase I clinical trial.
Provision of physiotherapy services at the sixth All Africa Games.
Authors: J Jelsma, H Dawson, G Smith, C Satumba, D Madzivire
Journal: British Journal of Sports Medicine (1997)

The provision of physiotherapy services to guest nations by the host nation, Zimbabwe, at the sixth All Africa Games is examined. There was a high rate of pre-existing injury. Ice, the Cryocuff, and ultrasound were the most frequently used tools, and should be made available to physiotherapists at all multisport events. It is recommended that collection of epidemiological data be standardised to allow comparison between events.
Prealbumin bearing lymphocytes as an indicator of hapten protein carrier in certain varieties of dermatitis
Authors: S. HUSZ, F. HAMERLINCK, R.H. CORMANE
Journal: British Journal of Dermatology (1975)

Patients with dermatoses where the antigen is a hapten, such as those with contact eczema and drug allergy, had high mean values of lymphocytes with membrane bound prealbumin detected by immunofluorescence methods. These results support the hypothesis that lymphocytes bind prealbumin. We speculate that this was haptenized prealbumin.
Abstract 4655: Gene expression signatures of microsatellite-stable stage III colorectal adenocarcinomas based on the <i>p53</i> status
Authors: Venkat R. Katkoori, Chandra K. Shanmugam, Dongquan Chen, Harvey L. Bumper, Tom Collens, Ludwine Messiaen, Upender Manne
Journal: Cancer Research (2010)

Abstract Background: Microsatellite (MS) instability and p53 mutations have been implicated in development, progression and metastasis of colorectal cancer (CRCs). Thus, to identify aggressive tumor subsets, the gene expression profiles of Stage- III MS-stable (MSS), sporadic CRCs were analyzed based on their p53 gene status. Methods: DNA samples extracted from 136 Stage-III formalin-fixed paraffin embedded CRC tissues were analyzed for MSS and for p53 mutations. Further, mRNA samples extracted from 20 (10 each of p53-mutated and wild-type) corresponding snap-frozen Stage-III-MSS-CRCs were profiled for differential gene expression. Affymetrix Human Genome U133 Plus 2.0 arrays were used for gene expression profiling; and Affymetrix Microarray Suite (MAS 5.0), gene ontology annotation, and KEGG pathways were used for data analysis. Differentially expressed (DE) genes were confirmed by measuring mRNA levels by RT-PCR and by the quantitative nuclease protection assay. Results: A higher incidence of p53 mutations was found in MSS (65 of 113, 58%) than in MSI (6 of 23, 30%) phenotypes. Kaplan-Meier analyses demonstrated that patients with MSS-CRCs exhibiting p53 mutations had poor overall survival when compared to p53-wt-phenotypes (log rank, p=0.031). Gene expression analyses identified 116 DE genes (73 down-regulated and 43 up-regulated). All of these passed the stringent background filter (intensity p-value ≤0.001 and intensity ≥3 SD above mean background) and DE ratio (χ2 p-value ≤0.01 &amp; fold change ≥2.0). GBP1, PSMB9, TRIM29, BST2 and TFF1, that have prognostic value in CRCs, were up-regulated in p53-mutated-MSS-CRCs but down-regulated in p53-wt-MSS-CRCs. Further, genes that regulate cell-cycle arrest (CDKN1B) and tumor growth suppression (ACVR1B, SESN1, WFDC1 and IRF7) were down-regulated in p53-mutated-MSS-CRCs, but up-regulated in p53-wt-MSS-CRCs. The genes presented here are the top-ranked (top-5) genes in their respective groups. The mRNA expression levels of these DE genes in all 136 CRCs were confirmed on two different platforms. Conclusion: Our findings suggest that mutant- and wt-p53 Stage-III-MSS-CRCs have distinct gene expression profiles and appear to be useful in identifying aggressive Stage III subsets and for the assessment of clinical outcomes. These studies are supported by grants from the National Institute of Health/National Cancer Institute (U54-CA118948, RO1-CA98932, and R03CA139629). Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4655.
Systemic allergic contact dermatitis due to ethylenediamine following administration of oral aminophylline
Authors: S.L. Walker, J.E. Ferguson
Journal: British Journal of Dermatology (2004)

Journal Article Systemic allergic contact dermatitis due to ethylenediamine following administration of oral aminophylline Get access S.L. Walker, S.L. Walker Dermatology Centre, Hope Hospital, Salford M6 8HD, U.K Dr S.L.Walker. E‐mail: drstevewalker@hotmail.com Search for other works by this author on: Oxford Academic Google Scholar J.E. Ferguson J.E. Ferguson Dermatology Centre, Hope Hospital, Salford M6 8HD, U.K Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 150, Issue 3, 1 March 2004, Page 594, https://doi.org/10.1111/j.1365-2133.2004.05796.x Published: 01 March 2004 Article history Accepted: 16 September 2003 Published: 01 March 2004
Enhanced intestinal glucose and alanine transport in cystic fibrosis.
Authors: P Baxter, J Goldhill, J Hardcastle, P T Hardcastle, C J Taylor
Journal: Gut (1990)

The rise in short-circuit current associated with the active transport of glucose and alanine was measured in intestinal biopsy samples from children with cystic fibrosis. The glucose-induced increase in the short-circuit current was greater in the cystic fibrosis tissues than in control samples over the whole range of concentrations tested (2.5-35 mM), a reflection of an increased maximum rate of transport. Similar results were obtained with alanine. These findings suggest that active Na(+)-linked nutrient transport is enhanced in cystic fibrosis.
ADAMTS13 Antibody Depletion by Bortezomib in Thrombotic Thrombocytopenic Purpura
Authors: Jake Shortt, Danielle H Oh, Stephen S Opat
Journal: New England Journal of Medicine (2013)

In some patients with refractory thrombotic thrombocytopenic purpura (TTP), efforts to reduce the causative antibody to ADAMTS13 by depleting B cells with rituximab are often effective. In a patient in whom such therapy failed, bortezomib was effective.
Tumor Antigen Expression in Melanoma Varies According to Antigen and Stage
Authors: Catherine Barrow, Judy Browning, Duncan MacGregor, Ian D. Davis, Sue Sturrock, Achim A. Jungbluth, Jonathan Cebon
Journal: Clinical Cancer Research (2006)

Abstract Purpose: Melanoma cells express antigens that can induce T-cell and antibody responses. Obtaining a detailed understanding of antigen expression in primary and metastatic melanoma is essential if these molecules are to be useful targets for immunotherapy of melanoma. Experimental Design: Malignant melanomas (n = 586) from 426 patients were typed for antigen expression. Multiple samples were available from 86 individuals, enabling analysis of antigen expression patterns over time. Paraffin-embedded samples were tested by immunohistochemistry for the presence of the differentiation antigens: gp100, Melan-A, tyrosinase, and the “cancer/testis” antigens MAGE-A1, MAGE-A4, and NY-ESO-1. Results: Samples were primary tumors (n = 251), lymph node metastases (n = 174), s.c. metastases (n = 71), and distant metastases (n = 90). The differentiation antigens were strongly expressed in 93% to 95% of tumors regardless of stage. In contrast, the frequency of cancer/testis antigen expression in primary tumors for MAGE-A1, MAGE-A4, and NY-ESO-1 was lower (20%, 9%, and 45%, respectively). MAGE-A1 and MAGE-A4 were acquired with advancing disease (to 51% and 44% in distant metastases, respectively) but not NY-ESO-1, which remained positive in 45%. MAGE-A1 expression was twice as prevalent in ulcerated primaries as in nonulcerated primaries (30% versus 15%; P = 0.006) and in thicker as opposed to thin melanomas (26% versus 10%; P = 0.1). Conclusions: This large series describes patterns of antigen expression in melanoma and their evolution over time. This will help inform decisions about selection of patients and target antigens for melanoma immunotherapy clinical trials.
Binding of Insulin to Rat Pancreatic Islets: Comparison Between Pancreatic Human Insulin and Biosynthetic Human Insulin
Authors: E J Verspohl, H P T Ammon
Journal: Diabetes Care (1981)

Human pancreatic insulin, biosynthetic human insulin (BHI), and pork insulin were compared in terms of their binding characteristics to insulin receptors on rat pancreatic islets. There was no difference in binding or on biologic effect, i.e., ability to inhibit insulin secretion.
Laments and Ancient Wisdom
Authors: Ronald C. Michels
Journal: Annals of Internal Medicine (1990)

Ad Libitum15 June 1990Laments and Ancient Wisdom1 May 1981 to 26 October 1989Ronald C. Michels, MDRonald C. Michels, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-112-12-968 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Excerpt1. Restless child. One so young, so full of his person. Uninhibited spirit. Loving and loved. Why, Father?2. Your servant Hannah: "I prayed for this child; and the Lord hath given me my petition which I asked of him." (I Samuel 1:27)3. An angel. He had seen an angel in his room. That's what he told them. She believed it a guardian angel. Why, Father?4. Now he will give his angels charge over thee to keep thee in all thy ways. (Psalm 91:11)5. The joyous birth. The diabetes. Then the seizures, and now, the young life.... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: Ronald C. Michels, MDAffiliations: Parkersburg, West Virginia*From a poem by Hillel Zeithlin (1872-1943). In: Glatzer N, ed. Language of Faith. New York: Schockan Books, Inc.; 1967:104. Previousarticle Advertisement FiguresReferencesRelatedDetails Metrics 15 June 1990Volume 112, Issue 12Page: 968-968KeywordsBirthEpileptic seizures ePublished: 1 December 2008 Issue Published: 15 June 1990 PDF downloadLoading ...
STUDIES ON GASTRIC SECRETION
Authors: Theodore G. Klumpp, Morris A. Bowie
Journal: Journal of Clinical Investigation (1933)

In the voluminous literature on gastric function one traces the gradual destruction of the hope that gastric analysis can give exact diagnostic information.The literature reveals, instead, the enormous variability of functional activity of the normal stomach.Achlorhydria has been observed frequently in persons who continue to be healthy and otherwise entirely normal.In pernicious anemia achlorhydria is not always present, as the findings of Castle (1) and his associates indicate.When so-called hyperacidity is found, it is often regarded as the cause of certain symptoms, but is as often present without symptoms.Despite the fact that we cannot ordinarily establish a definite diagnosis on the basis of gastric analysis alone, it gives supportive information of unquestioned value.The fashion in test meals has, over a period of years, favored foods common to the usual dietary.The idea has been that bread and water, milk, broth or even a full meal offered a physiological stimulus, and such meals as the Ewald, Boas, Sahli and Riegel have enjoyed the favor of many years.In recent years a dilute solution of alcohol has gained vogue as a test substance because it is a clear solution and provokes a significant secretion of gastric juice.In 1920, the action of histamine on gastric secretion was first studied by Popielski (2).In 1922 stimulation of gastric secretion by histamine was applied to man by Carnot, Koskowski and Libert (3).Since then it has been increasingly used in gastric analysis, largely because it was thought to give a more intense, possibly maximum stimulation.At the present time, the Ewald, alcohol, and histamine stimuli are widely used in this country.They have been extensively studied, but these studies have yielded very little.information by means of which their actions can be adequately compared.This is due to the fact that insufficient allowance has been made for the wide range of functional variation of normal gastric secretion.In order to balance this variable in considering two test meals, it is necessary to give both of them to the same subject, under standard conditions, and compare adequate series of such duplicate analyses.With this in mind, a study of gastric function was undertaken.A total of three hundred and fifty-seven analyses were made on ninety-eight subjects under controlled conditions. 1
The Impact of Germline Genetic Variations in Hydroxysteroid (17-Beta) Dehydrogenases on Prostate Cancer Outcomes After Prostatectomy
Authors: Étienne Audet-Walsh, Judith Bellemare, Louis Lacombe, Yves Fradet, Vincent Fradet, Pierre Douville, Chantal Guillemette, Éric Lévesque
Journal: European Urology (2011)
Urokinase Pulmonary Embolism Trial
Authors: Francis Kuthan
Journal: JAMA (1971)

<h3>To the Editor.—</h3> A 12-hour infusion of urokinase was shown to accelerate the resolution rate of pulmonary thromboemboli more than did a similar infusion of heparin (<b>214:</b>2163, 1970). However, "... urokinase regimen did not usually achieve complete thrombolysis...." The lack of dramatic thrombolysis may well be ascribed to the short duration of the urokinase infusion. In a study of treatment of acute thrombophlebitis of the deep iliac, femoral, and leg veins with streptokinase, Widmer<sup>1</sup>et al showed that it was necessary to infuse the agent for as long as three to seven days in a majority of the patients treated. The conclusions of that study were based exclusively on phlebography, rather than upon unreliable clinical signs only. Discontinuation of the streptokinase infusion was effected only after complete thrombus resolution had been found on phlebography, after laboratory tests had shown a considerable decrease in the activity of the fibrinolytic system,
Mortality From Abortion and Childbirth
Authors: Scot A. LeBolt
Journal: JAMA (1982)

Critics have challenged previous comparisons of mortality from legal abortion and childbirth for contrasting population groups with different clinical characteristics. They allege that most women dying from abortion were young, white, and healthy, while those dying from childbirth had serious underlying conditions. To address this question, we calculated standardized abortion and childbirth mortality rates between 1972 and 1978. We also adjusted independently for preexisting medical conditions. These adjustments for demographic and health differences between the two populations actually widened the difference in the mortality risk between abortion and childbirth. Thus, between 1972 and 1978, women were about seven times more likely to die from childbirth than from legal abortion, with the gap increasing in the more recent years. (<i>JAMA</i>1982;248:188-191)
Potential Role of Jun Activation Domain–Binding Protein 1 as a Negative Regulator of p27kip1 in Pancreatic Adenocarcinoma
Authors: Maria A. Kouvaraki, Anita L. Korapati, George Z. Rassidakis, Ling Tian, Qingxiu Zhang, Paul Chiao, Linus Ho, Douglas B. Evans, François X. Claret
Journal: Cancer Research (2006)

Abstract Reduced expression of p27 has been associated with poor prognosis in most human cancers, including pancreatic adenocarcinoma. Jun activation domain–binding protein 1 (JAB1), an activator protein (AP-1) coactivator, previously implicated in p27 degradation, is overexpressed in various tumors and correlates with low p27 expression. We examined JAB1 and p27 in normal and neoplastic pancreatic tissues. Increased JAB1 expression was seen in pancreatic carcinoma samples but not in paired normal pancreatic tissues. Immunohistochemical analysis using tissue microarrays showed that JAB1 was overexpressed in all 32 (100%) pancreatic adenocarcinoma samples tested, predominantly nuclear in 23 (72%) samples and predominantly cytoplasmic in 9 (28%) tumors. When 10% was used as a cutoff for p27 positivity, p27 was expressed in 11 (34%) of tumors; however, p27 expression was localized in the nuclei of tumor cells in only 4 (13%) of the samples. Overexpression of the JAB1 in the pancreatic carcinoma cell lines Panc-1, Mia PaCa-2, and Panc-28 resulted in decreased p27 expression. Conversely, down-regulation of JAB1 by short interfering RNA substantially increased p27 expression and inhibited progression from G1 to S phase of the cell cycle. Interestingly, JAB1-mediated p27 degradation was not impaired when S-phase kinase-interacting protein 2 (Skp2), an F-box protein required for the ubiquitination and consequent degradation of p27, was silenced. Thus, JAB1 may have an Skp2-independent p27 degradation mechanism in pancreatic cancer cells. These findings suggest that JAB1 overexpression is involved in the pathogenesis of pancreatic cancer through JAB1-mediated p27 degradation and that control of JAB1 expression is a novel therapeutic target in patients with pancreatic adenocarcinomas. (Cancer Res 2006; 66(17): 8581-9)
Transient response of pure red cell aplasia to anti‐thymocyte globulin in a patient with T‐cell chronic lymphocytic leukemia
Authors: William Hocking, Richard Champlin, Ronald Mitsuyasu
Journal: American Journal of Hematology (1987)

Abstract Pure red cell aplasia (PRCA) is an unusual complication of chronic lymphoproliferative disorders. A patient with T‐cell chronic lymphocytic leukemia (T‐CLL) had severe anemia and neutropenia. Initial in vitro studies demonstrated no evidence of T‐cell suppression of erythropoiesis. Sequential bone marrow examinations demonstrated progressive red cell aplasia. In vitro studies showed that the T‐cells from the patient suppressed allogeneic but not autologous BFU‐E. Treatment with antithymocyte globulin (ATG) reduced circulating leukemic cells and produced a definite but transient improvement in erythropoiesis.
Medical Education Tomorrow
Authors: Chester S. Keefer
Journal: New England Journal of Medicine (1956)

WHEN this association was founded in 1886, with the object of advancing scientific and practical medicine, there was very little that could be called scientific medicine as it is known today. Practical medicine was based largely upon tradition, magic and empiricism. Medical education was carried out for the most part in schools where the preceptorship and the lecture were emphasized, and university medical education was largely undeveloped or nonexistent.About the time of the first meeting, however, a new ferment was working, and a revolution was being introduced into all science, especially the biologic sciences. This revolution has proved to . . .
B7-H3 expression in donor T cells and host cells negatively regulates acute graft-versus-host disease lethality
Authors: R. G. Veenstra, R. Flynn, K. Kreymborg, C. McDonald-Hyman, A. Saha, P. A. Taylor, M. J. Osborn, A. Panoskaltsis-Mortari, A. Schmitt-Graeff, E. Lieberknecht, W. J. Murphy, J. S. Serody, D. H. Munn, G. J. Freeman, J. P. Allison, T. W. Mak, M. van den Brink, R. Zeiser, B. R. Blazar
Journal: Blood (2015)
"Hot" Spleen on Tc-99m Sulfur Colloid Images
Authors: DOROTHY S. LIN
Journal: Clinical Nuclear Medicine (1983)

In routine Tc-99m sulfur colloid (SC) liver-spleen imaging, relatively increased splenic uptake when compared with liver uptake is frequently seen. Commonly this is due to decreased hepatic uptake secondary to liver disease, but often it is because of increased uptake in the hyperfunctioning spleen. Reported is a case of lymphoma of the tonsil, with a “hot” spleen which was free of tumor. Possible etiology of the “hot” spleen is discussed and the known causes listed according to their prevalence.