tbuckley/cabot-search huggingface dataset:
Effect of Anesthetic Agent on Lung Tumor Induction in Hamsters Given Benzo[a]pyrene-Ferric Oxide2
Authors: M. C. Henry, C. D. Port
Journal: JNCI Journal of the National Cancer Institute (1978)

Journal Article Effect of Anesthetic Agent on Lung Tumor Induction in Hamsters Given Benzo[a]pyrene-Ferric Oxide Get access M. C. Henry, M. C. Henry 3Life Sciences Division, Illinois Institute of Technology, Chicago, III. 60616. 4present address: Environmental Protection Research Division, U.S. Army Medical Bioengineering Research and Development Laboratory, Ft. Detrick, Md. 21701. Address reprint requests to M. C. Henry Search for other works by this author on: Oxford Academic PubMed Google Scholar C. D. Port C. D. Port 3Life Sciences Division, Illinois Institute of Technology, Chicago, III. 60616. 5Present address: Department of Pathology, Northwestern University Medical School, 303 East Chicago Ave., Chicago, Ill. 60611. Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 61, Issue 5, November 1978, Pages 1221–1227, https://doi.org/10.1093/jnci/61.5.1221 Published: 01 November 1978 Article history Received: 05 January 1978 Accepted: 14 June 1978 Published: 01 November 1978
BALB/c and C57BL/6 Mice Differ in Polyreactive IgA Abundance, which Impacts the Generation of Antigen-Specific IgA and Microbiota Diversity
Authors: Floris Fransen, Elena Zagato, Elisa Mazzini, Bruno Fosso, Caterina Manzari, Sahar El Aidy, Andrea Chiavelli, Anna Maria D’Erchia, Maya K. Sethi, Oliver Pabst, Marinella Marzano, Silvia Moretti, Luigina Romani, Giuseppe Penna, Graziano Pesole, Maria Rescigno
Journal: Immunity (2015)
Prostaglandin F2 alpha- and 12-O-tetradecanoylphorbol-13-acetate-induced alterations in the pathways of renal ammoniagenesis.
Authors: A Sahai, I Nissim, R S Sandler, R L Tannen
Journal: Journal of the American Society of Nephrology (1995)

The mechanisms whereby prostaglandin F2 alpha (PGF2 alpha) and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibit ammoniagenesis and the reason why they behave differently at pH 7.4, were examined with (15N)glutamine to assess the metabolic pathways and 2'-7'-bis(2-carboxyethyl)-5-(and-6)-carboxylfluorescein, acetoxymethylester (BCECF-AM) to evaluate Na+/H+ antiporter activity. LLC-PK1 cultures were incubated for 1 h in a Krebs-Hensleit bicarbonate buffer of pH 7.4 and pH 6.8 supplemented either with 5-15N- or 2-15N-labeled glutamine, followed by the assessment of (15N)ammonia and (15N)amino acid formation. Exposure of cells to either PGF2 alpha or TPA completely inhibited the low pH-induced increases in (15N)ammonia formation from incubations with 5-15N, reflecting reduced flux through the mitochondrial phosphate-dependent glutaminase, and from (2-15N)glutamine, reflecting reduced flux through the mitochondrial glutamate dehydrogenase pathway. They also qualitatively reversed the acute acidosis-induced changes in (15N)alanine formation and (15N)glutamate accumulation in the media. By contrast only TPA, but not PGF2 alpha, modified glutamine metabolism at pH 7.4. Na+/H+ antiporter activity was assessed under both acidified and basal (pH 7.4) conditions by measuring changes in intracellular pH in cells loaded with BCECF. TPA and PGF2 alpha both stimulated Na+/H+ antiporter activity comparably under acidified conditions. When cells were studied at pH 7.4, TPA but not PGF2 alpha stimulated the Na+/H+ antiporter and increased steady-state intracellular pH.(ABSTRACT TRUNCATED AT 250 WORDS)
Neuropeptide Y expression distinguishes malignant from benign pheochromocytoma.
Authors: L J Helman, P S Cohen, S D Averbuch, M J Cooper, H R Keiser, M A Israel
Journal: Journal of Clinical Oncology (1989)

No reliable gross or microscopic features distinguish benign from malignant pheochromocytomas. The diagnosis of malignant pheochromocytoma is based solely on the presence of regional or distant metastases. This study evaluated the expression of neuropeptide Y messenger RNA (mRNA) in nine benign and 11 malignant pheochromocytomas and has found that neuropeptide Y mRNA was expressed in all nine benign tumors but in only four of 11 malignant tumors (P = .0084). These data suggest that the determination of neuropeptide Y expression in the evaluation of patients with pheochromocytoma may have prognostic significance.
Tetraspanin CD81 Promotes Tumor Growth and Metastasis by Modulating the Functions of T Regulatory and Myeloid-Derived Suppressor Cells
Authors: Felipe Vences-Catalán, Ranjani Rajapaksa, Minu K. Srivastava, Aurelien Marabelle, Chiung-Chi Kuo, Ronald Levy, Shoshana Levy
Journal: Cancer Research (2015)

Abstract Tumor cells counteract innate and adaptive antitumor immune responses by recruiting regulatory T cells (Treg) and innate myeloid-derived suppressor cells (MDSC), which facilitate immune escape and metastatic dissemination. Here we report a role in these recruitment processes for CD81, a member of the tetraspanin family of proteins that have been implicated previously in cancer progression. We found that genetic deficiency in CD81 reduced tumor growth and metastasis in two genetic mouse backgrounds and multiple tumor models. Mechanistic investigations revealed that CD81 was not required for normal development of Treg and MDSC but was essential for immunosuppressive functions. Notably, adoptive transfer of wild-type Treg into CD81-deficient mice was sufficient to promote tumor growth and metastasis. Our findings suggested that CD81 modulates adaptive and innate immune responses, warranting further investigation of CD81 in immunomodulation in cancer and its progression. Cancer Res; 75(21); 4517–26. ©2015 AACR.
Identification of Bone-Derived Factors Conferring <i>De Novo</i> Therapeutic Resistance in Metastatic Prostate Cancer
Authors: Yu-Chen Lee, Song-Chang Lin, Guoyu Yu, Chien-Jui Cheng, Bin Liu, Hsuan-Chen Liu, David H. Hawke, Nila U. Parikh, Andreas Varkaris, Paul Corn, Christopher Logothetis, Robert L. Satcher, Li-Yuan Yu-Lee, Gary E. Gallick, Sue-Hwa Lin
Journal: Cancer Research (2015)

Abstract Resistance to currently available targeted therapies significantly hampers the survival of patients with prostate cancer with bone metastasis. Here we demonstrate an important resistance mechanism initiated from tumor-induced bone. Studies using an osteogenic patient-derived xenograft, MDA-PCa-118b, revealed that tumor cells resistant to cabozantinib, a Met and VEGFR-2 inhibitor, reside in a “resistance niche” adjacent to prostate cancer-induced bone. We performed secretome analysis of the conditioned medium from tumor-induced bone to identify proteins (termed “osteocrines”) found within this resistance niche. In accordance with previous reports demonstrating that activation of integrin signaling pathways confers therapeutic resistance, 27 of the 90 osteocrines identified were integrin ligands. We found that following cabozantinib treatment, only tumor cells positioned adjacent to the newly formed woven bone remained viable and expressed high levels of pFAK-Y397 and pTalin-S425, mediators of integrin signaling. Accordingly, treatment of C4-2B4 cells with integrin ligands resulted in increased pFAK-Y397 expression and cell survival, whereas targeting integrins with FAK inhibitors PF-562271 or defactinib inhibited FAK phosphorylation and reduced the survival of PC3-mm2 cells. Moreover, treatment of MDA-PCa-118b tumors with PF-562271 led to decreased tumor growth, irrespective of initial tumor size. Finally, we show that upon treatment cessation, the combination of PF-562271 and cabozantinib delayed tumor recurrence in contrast to cabozantinib treatment alone. Our studies suggest that identifying paracrine de novo resistance mechanisms may significantly contribute to the generation of a broader set of potent therapeutic tools that act combinatorially to inhibit metastatic prostate cancer. Cancer Res; 75(22); 4949–59. ©2015 AACR.
Epidemiological characterization and distribution of carbapenem-resistant Acinetobacter baumannii clinical isolates in Italy
Authors: M.L. Mezzatesta, M.M. D'Andrea, R. Migliavacca, T. Giani, F. Gona, E. Nucleo, G. Fugazza, L. Pagani, G.M. Rossolini, S. Stefani
Journal: Clinical Microbiology and Infection (2011)
Abstract 277: Supraglottic Airway Use Is Associated with Higher Chest Compression Fraction than Endotracheal Intubation During Out-of-Hospital Cardiopulmonary Arrest
Authors: Michael C Kurz, David Prince, J Christenson, J Carlson, S May, D Stub, S Cheskes, S Lin, Michael Aziz, Michael Austin, C Vaillancourt, J Colvin, Henry E Wang
Journal: Circulation (2014)

Objective: Chest compression interruptions - such as those from endotracheal intubation (ETI) - are associated with poorer out-of hospital cardiac arrest (OHCA) survival. Select Emergency Medical Services (EMS) practitioners substitute ETI with supraglottic airway (SGA) insertion to minimize these interruptions, but the resulting effects upon chest compression fraction (CCF) are unknown. We sought to determine the differences in CCF between adult OHCA receiving ETI and those receiving SGA. Methods: We studied adult, non-traumatic OHCA patients enrolled in the Resuscitation Outcomes Consortium (ROC) PRIMED trial. Chest compressions were measured using compression or thoracic impedance sensors. We limited the analysis to those receiving ETI or SGA and &gt;2 minutes of chest compression data before and after airway insertion. We compared CCF between ETI and SGA before and after airway insertion, adjusting for age, sex, witnessed arrest, bystander CPR, shockable initial rhythm, public location, PRIMED trial arm, and regional ROC center. We also compared the change in CCF for each airway technique and stratified these analyses by initial rhythm. We analyzed the data using t-tests and multivariable linear regression. Results: Of 14,955 patients enrolled in the ROC PRIMED trial, we analyzed 2,767 cases, including 2051 ETI, 671 SGA, and 45 both. Unadjusted pre- and post- airway CCF was higher for SGA than ETI (pre- 0.732 vs 0.706, difference -0.026 95% CI -0.044, -0.008; post- 0.767 vs 0.724, difference -0.043 95% CI -0.060, -0.026). Adjusted post-airway CCF improved with both techniques, but the changes were not statistically significant (0.012 difference, 95% CI 0.036, -0.012, p-value 0.32). CCF differences were similar when stratified by initial rhythm. Conclusion: In this series SGA insertion was associated with a higher CCF than ETI and that difference persisted post-airway insertion. Advanced airway management strategy may minimally impact CCF.
Abstract 15577: The Activation of Nlrp3 Inflammasome Mediates Diet- and Chlamydia Pneumonia- Induced Acceleration of Atherosclerosis by Th17 Skewing
Authors: Gantsetseg Tumurkhuu, Wenxuan Zhang, Kenichi Shimada, Ganghua Huang, Timothy Crother, Moshe Arditi, Shuang Chen
Journal: Circulation (2014)

Background: The role of Nlrp3 inflammasome activation in the development of atherosclerosis in hypercholesterolemic mice is still controversial, and its role in Chlamydia pneumonia (Cp) infection accelerated atherosclerosis is also unclear. Objective: We investigated the role of the Nlrp3 inflammasome in high-fat diet (HFD) and Cp induced acceleration of atherosclerosis and its potential mechanism. Methods and Results: We created 3 groups of Bone Marrow chimeric (BMC) mice (Donor → Recipient): WT →Ldlr-/- ; Nlrp3-/- → Ldlr-/- ; Casp1-/- → Ldlr-/-. After 16 wks HFD, quantification of the lesion area of aortic sinus plaques and lipid content revealed a significant reduction in lesion size and lipid content in the aortic root and in aorta en face in Nlrp3-/- (25%, 32%, 25%), and Casp1-/- (26%, 32%, 26%) BMC compared to WT BMC mice, despite similar cholesterol levels. In next experiments, mice were infected with Cp or vehicle once a week for 3 wks and fed HFD for 16 wks before sacrificing. Nlrp3-/- and Casp1-...
Nasal and bronchial levels of Th2 cytokines correlate during a virus induced asthma exacerbation
Authors: David Jackson, Maria-Belen Trujillo-Torralbo, Betty Shamji, Jerico Del Rosario, Duncan Hunt, Toby Hunt, Trevor Hunt, Onn Min Kon, Matt Edwards, John Westwick, Trevor Hansel, Sebastian Johnston
Journal: European Respiratory Journal (2012)

Asthma is a heterogeneous condition and it is vital to accurately predict responders to targeted therapies. However, difficulties in measuring IL5 and IL13 have forced reliance on indirect markers of Th2 inflammation with limited success. Using the human model of experimental rhinovirus (RV) induced asthma exacerbation (AE) and new techniques to absorb nasal (nasosorption) and bronchial (bronchosorption) mucosal lining fluid (MLF), we explored Th2 inflammation during a RV-induced AE.  Methods: 32 mild-to-moderate asthmatics and 14 healthy subjects were inoculated with RV-16. Bronchoscopies were performed 2 weeks prior to inoculation and on d4 post-inoculation. Cytokines were measured in both bronchial and nasal samples at baseline and on d4 with further nasal sampling on days 2,3,5,7,10 and 42.  Results: Nasal IL5 and IL13 were significantly increased in asthma during infection compared to baseline (p  Conclusion: RV induced Th2 inflammation correlated with AE severity. Nasal Th2 inflammation correlated with bronchial levels whilst baseline Th2 levels predicted the magnitude of Th2 induction during the AE. Nasosorption is a non-invasive, rapid technique capable of measuring Th2 inflammation directly. It may be possible to use this technique as a biomarker to guide therapy with anti-IL5 and anti-IL13 mAb treatments.
Chest CT findings in patients with angioimmunoblastic T-cell lymphoma
Authors: Hisako Kushima, Hiroshi Ishii, Kosaku Komiya, Hiroaki Oka, Issei Tokimatsu, Jun-ichi Kadota
Journal: European Respiratory Journal (2012)

Background:  Angioimmunoblastic T-cell lymphoma (AILT), a subtype of peripheral T-cell lymphoma, is an aggressive disorder representing approximately 2% of non-Hodgkin lymphomas. Most of the patients with AILT present with pyrexia, weight loss, lymphadenopathy, splenomegaly, skin rushes, and various thoracic involvements. However, there have been few reports of thoracic images in patients with AILT.   Objective:  To determine the characteristics of the thoracic involvements in AILT.   Method:  The clinical and radiological data of 7 patients [6 males and 1 female, median age: 74 (65-78) years old] with pathologically-diagnosed AILT between 1998 and 2011 were retrospectively analyzed.   Results:  The most common manifestations in onset were pyrexia and swelling of the cervical lymph nodes. Radiographic findings of the chest included mediastinal and hilar lymphadenopathy (seen in 7/7 patients), pleural effusion (5/7), thickening of the bronchovascular bundles (5/7), ground-glass opacities (5/7), consolidation (2/7), and pericardial effusion (1/7). Two patients (2/7) developed interstitial pneumonia after chemotherapy-induced remission, and one of the two patients underwent a surgical lung biopsy and showed cellular and organizing interstitial pneumonia.   Conclusion:  AILT is a rare type of non-Hodgkin lymphoma, however, chest physicians should be aware that thoracic involvements are frequently seen in this disease.
Acquired tufted angioma: a unique vascular lesion not previously reported in the oral mucosa
Authors: C.L. Kleinegger, H.L. Hammond, S.D. Vincent, M.W. Finkelstein
Journal: British Journal of Dermatology (2000)

Journal Article Acquired tufted angioma: a unique vascular lesion not previously reported in the oral mucosa Get access C.L. Kleinegger, C.L. Kleinegger Department of Oral Pathology, Radiology and Medicine, College of Dentistry, University of Iowa, Iowa City, IA 52242‐1001, U.S.A. C.L.Kleinegger. E‐mail: cynthia‐kleinegger@uiowa.edu Search for other works by this author on: Oxford Academic Google Scholar H.L. Hammond, H.L. Hammond Department of Oral Pathology, Radiology and Medicine, College of Dentistry, University of Iowa, Iowa City, IA 52242‐1001, U.S.A. Search for other works by this author on: Oxford Academic Google Scholar S.D. Vincent, S.D. Vincent Department of Oral Pathology, Radiology and Medicine, College of Dentistry, University of Iowa, Iowa City, IA 52242‐1001, U.S.A. Search for other works by this author on: Oxford Academic Google Scholar M.W. Finkelstein M.W. Finkelstein Department of Oral Pathology, Radiology and Medicine, College of Dentistry, University of Iowa, Iowa City, IA 52242‐1001, U.S.A. Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 142, Issue 4, 1 April 2000, Pages 794–799, https://doi.org/10.1046/j.1365-2133.2000.03429.x Published: 01 April 2000
Different effects of mesenchymal stem cells on elastase-induced pulmonary emphysema and macrophages activity in rats
Authors: Alexander Averyanov, Anatoly Konoplyannikov, Andrey Chernyaev, Vasily Petrov, Oleg Kuzovlev, Andrey Bruchovetsky, Olga Konoplyannikova, Ekaterina Agaeva, Natalia Kulagina
Journal: European Respiratory Journal (2011)

The aim of the study was to assess lung tissue changes and macrophages activity (MA) after mesenchymal stem cells (MSCs) transplantation in elastase model of pulmonary emphysema in rats.   Methods:  Forty 3-months old Wistar rats were randomized into 4 groups. Control group (group 1) was injected intratracheally 0,4 ml of normal saline, other animals (groups 2-4 ) received one intratracheal injection of 20 units (U) porcine pancreatic elastase in 0,4 ml of saline. Next day (group 2) and 7 day (group 4) rats were intravenously injected 2000000 autologous MSCs in 0,5 ml of saline. Group 4 was used as emphysema control. Before killing on 21-st day rats were undergone peritoneal lavage with analysis of hemi-luminescent macrophages activity in the obtained fluid   Results:  The lungs of groups 2-4 had various degrees of centriacinar emphysema. There were no significant differences between the sizes of dilated respiratory bronchioles in groups 2 & 4. Only in the 2-nd group we observed the lymphoid infiltration around arterioles. The width of respiratory bronchioles in group 3 was on 41% less than in the 4-th. The Index of hemi-luminescent MA has made 28,8±1,1 U (1 group), 35,8±1,6 U (2 group), 31,9±1,9 U (group 3), 57,3±1,3 U (group 4).   Conclusions:  MSCs have various effects on damaged lung tissue at different periods of transplantation. MSCs can enhance the local vascular inflammatory response in the early period of elastase injury. More later injection of MSCs can repair the structure of acini in acute model of emphysema in rats.
A Preliminary Pharmacokinetic Study of Dianhydrogalactitol (NSC-132313) Disposition in the Dog2
Authors: Toshikiro Kimura, Larry A. Sternson, Valentino J. Stella, Takeru Higuchi
Journal: JNCI Journal of the National Cancer Institute (1977)

Journal Article A Preliminary Pharmacokinetic Study of Dianhydrogalactitol (NSC-132313) Disposition in the Dog Get access Toshikiro Kimura, Toshikiro Kimura Search for other works by this author on: Oxford Academic PubMed Google Scholar Larry A. Sternson, Larry A. Sternson Search for other works by this author on: Oxford Academic PubMed Google Scholar Valentino J. Stella, Valentino J. Stella Search for other works by this author on: Oxford Academic PubMed Google Scholar Takeru Higuchi Takeru Higuchi Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 58, Issue 5, May 1977, Pages 1311–1314, https://doi.org/10.1093/jnci/58.5.1311 Published: 01 May 1977 Article history Received: 17 May 1976 Accepted: 03 November 1976 Published: 01 May 1977
Hypercalciuria
Authors: M J Favus
Journal: Journal of the American Society of Nephrology (1994)

Human idiopathic hypercalciuria (IH) is a common cause of hypercalciuria that contributes to calcium oxalate nephrolithiasis. The disorder is characterized by normocalcemia, increased intestinal Ca absorption, and normal or elevated circulating 1,25(OH)2D3. Intestinal Ca hyperabsorption, which is a source of excess urine Ca excretion, may result from either a primary increase in renal 1,25(OH)2D3 production; a primary, vitamin D-independent defect in enterocyte regulation of Ca transport; or a secondary increase in 1,25(OH)2D3 production in response to a defect in renal tubular Ca reabsorption. Breeding male and female Sprague Dawley rats with spontaneous hypercalciuria has resulted in offspring with hypercalciuria, increased intestinal Ca absorption, and normal serum 1,25(OH)2D3. In male IH rats, vitamin D receptor (VDR) content measured by saturation binding and western blotting revealed a twofold increase in VDR number in the duodenum, kidney cortex, and splenic monocytes. The molecular basis for the increase in VDR appears not to be due to increased VDR gene expression, but may result from increased efficiency of translation of the VDR message or prolongation of the half-life of VDR. Comparable migration of normal and IH intestinal VDR on western blots and of intestinal VDR mRNA on northern blots suggests that the abundant VDR in IH rat intestine is not a mutation of the wild-type VDR. These observations strongly suggest that, in IH rats, normal serum 1,25(OH)2D3 and increased VDR results in increased VDR-1,25(OH)2D3 complexes and enhanced biologic actions of 1,25(OH)2D3, including increased intestinal Ca transport. IH in rats may be the first genetic disorder due to a pathologic increase in the VDR.
Impaired neutrophil phagocytosis and receptor expression in non-CF bronchiectasis
Authors: James D. Chalmers, Adler Ma, Kim Turnbull, Catherine Doherty, John R. Govan, Adam Hill
Journal: European Respiratory Journal (2013)

Introduction

Large numbers of neutrophils are present in the bronchiectasis airway. Why these neutrophils fail to phagocytose and kill bacteria is unclear.

Methods

Peripheral blood and sputum neutrophils were isolated from 23 patients with bronchiectasis. Flow cytometry was used to assess neutrophil receptor expression, oxidative burst and phagocytosis of FITC-labelled P. aeruginosa. These were compared to peripheral blood neutrophils from 9 healthy control subjects. Patients were classified as mild/moderate or severe bronchiectasis based on HRCT scoring and the presence of P. aeruginosa.

Results

No differences were observed in peripheral blood neutrophils between bronchiectasis patients and controls. Sputum neutrophils from patients showed reduced expression of FcγRIIIb, complement receptor 1 (CR1) and CD88 compared to control neutrophils. Expression correlated with severity of bronchiectasis- figure 1.

![Figure][1] 



Severe bronchiectasis was associated with reduced oxidative burst and reduced phagocytosis.

Decreased expression of FcγRIIIb and CD88 were observed after incubating peripheral blood neutrophils with patient sputum sol and was prevented by pre-treating sputum with the specific elastase inhibitor MeOSu-AAPV-CMK.

Conclusion

Airway neutrophils in bronchiectasis show reduced phagocytosis, correlated with reduced phagocytic receptor expression. Neutrophil elastase is a major mediator of airway neutrophil dysfunction in bronchiectasis.

 [1]: pending:yes