tbuckley/cabot-search huggingface dataset:
In the Meantime, Life
Authors: Carol Snyder Halberstadt
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Cytotoxicity of Heterologous Immune Chicken Serum to Normal and Neoplastic Mouse Tissues in Culture&lt;xref ref-type="fn" rid="FN1"&gt;2&lt;/xref&gt;
Authors: Peter Abramoff, John W. Saunders, Mary T. Gasseling
Journal: JNCI Journal of the National Cancer Institute (1961)

New Hampshire Red cockerels were immunized against the Sarcoma 180 mouse tumor and against tissues of normal mice of its BALB/c host. Plasmas and serums from immunized and nonimmunized chickens were used in tissue cultures of the tumor and of embryonic mouse tissues. Antibodies against the tumors showed cytotoxic effects on tumor cells even after absorption with normal mouse antigens, but did not affect embryonic cells. Similar effects were not shown by antibodies against normal mouse tissues.
Abstract 13363: Cardiac Rubidium-82 Positron Emission Tomography as an ‘Area at Risk’ Marker. Comparison to the Gold Standard, Cardiac SPECT
Authors: Adam A Ghotbi, Philip Hasbak, Thomas Christensen, Thomas Engstrøm, Henning Kelbæk, Andreas Kjær
Journal: Circulation (2014)

Introduction: Determination of the area at risk (AaR) is essential in assessing myocardial salvage, which is an important surrogate marker when assessing new cardioprotective strategies. Single-photon emission computed tomography (SPECT) is considered the gold standard for measuring these parameters, however, it is logistically challenging and requires tracer injection prior to revascularization. Hypothesis: We hypothesized that Rubidium (Rb)-82 Positron Emission Tomography (PET) can determine the AaR in myocardial infarction after the initial treatment with percutaneous coronary intervention. Methods: Twelve patients with ST-elevation myocardial infarction were prospectively enrolled and prior to revascularization injected with Tc-99m-Sestamibi and after revascularization underwent cardiac SPECT imaging followed by cardiac Rb-82 PET. Quantitation of perfusion defect from both PET and SPECT was determined by an automated algorithm with a threshold of <50 % of normal counts considered as AaR and estimated ...
Qualitative research in health care: Analysing qualitative data
Authors: C. Pope
Journal: BMJ (2000)

This is the second in a series of three articles 

Contrary to popular perception, qualitative research can produce vast amounts of data. These may include verbatim notes or transcribed recordings of interviews or focus groups, jotted notes and more detailed “fieldnotes” of observational research, a diary or chronological account, and the researcher's reflective notes made during the research. These data are not necessarily small scale: transcribing a typical single interview takes several hours and can generate 20–40 pages of single spaced text. Transcripts and notes are the raw data of the research. They provide a descriptive record of the research, but they cannot provide explanations. The researcher has to make sense of the data by sifting and interpreting them.

#### Summary points

Qualitative research produces large amounts of textual data in the form of transcripts and observational fieldnotes

The systematic and rigorous preparation and analysis of these data is time consuming and labour intensive

Data analysis often takes place alongside data collection to allow questions to be refined and new avenues of inquiry to develop

Textual data are typically explored inductively using content analysis to generate categories and explanations; software packages can help with analysis but should not be viewed as short cuts to rigorous and systematic analysis

High quality analysis of qualitative data depends on the skill, vision, and integrity of the researcher; it should not be left to the novice

In much qualitative research the analytical process begins during data collection as the data already gathered are analysed and shape the ongoing data collection. This sequential analysis1 or interim analysis2 has the advantage of allowing the researcher to go back and refine questions, develop hypotheses, and pursue emerging avenues of inquiry in further depth. Crucially, it also enables the researcher to look for deviant or negative cases; that is, …
Abstract 2625: Increased sensitivity to Wnt/beta-catenin inhibitors in hepatocellular carcinoma cells harboring activating mutation of beta-catenin
Authors: Hsiao-Hui Lin, Wen-Chi Feng, LI-Chun Lu, Ta-Wen Hsu, Ann-Lii Cheng, Chih-Hung Hsu
Journal: Cancer Research (2014)

Abstract [Background] The WNT/β-catenin signaling pathway is a major oncogenic pathway of human hepatocellular carcinoma (HCC). More than 50% of HCCs have aberrant activation of WNT/β-catenin signaling, which are caused by genetic alterations, ligand activation, or cross-activation by other signaling pathways. We hypothesized that activating mutation of CTNNB1, the coding gene of β-catenin, may confer sensitivity to WNT/β-catenin inhibitors in HCC cells. [Materials and Methods] SNU398 is a human HCC cell line harboring a missense somatic mutation in exon 3 of CTNNB1 gene, resulting in S37C mutation. Huh7, HepG2, PLC5, and Hep3B are HCC cells containing no somatic mutations in exon 3 of CTNNB1 gene. HCC cells were treated with various types of WNT/β-catenin pathway inhibitors, including ICG-001 which down-regulates β-catenin/TCF transcriptional activity by interfering the binding of β-catenin with its transcriptional activation complex, XAV939 which targets tankyrases and facilitates degradation of β-catenin, and LGK974 which inhibits Porcupine, an acyltransferase responsible for palmitoylation and secretion of Wnt ligands, and evaluated for in vitro viability by MTT. [Results] ICG-001 as a single agent showed significantly greater anti-proliferation effect in SNU398 than other HCC cells. The 50%-inhibitory concentrations (IC50s) of ICG-001 were 5 μM and &amp;gt;10 μM for SNU398 and other HCC cells, respectively. ICG-001 also induced a more potent suppression of colony- formation, and a more significant increase of subG1 fraction detected by flow cytometry in SNU398 cell than other HCC cells. XAV939 up to 20 μM induced only modest anti-proliferative effect in all HCC cells. However, SNU398 remained the one showing the greatest sensitivity to the growth suppression induced by XAV939. The anti-proliferative effect of LGK974 was modest and showed no significant difference among different HCC cells. [Conclusion] The activating mutation of β-catenin may confer sensitivity to certain WNT/β-catenin pathway inhibitors, such as ICG-001, in HCC cells. (This study was supported by grants NRPB-100CAP1020-2). Citation Format: Hsiao-Hui Lin, Wen-Chi Feng, LI-Chun Lu, Ta-Wen Hsu, Ann-Lii Cheng, Chih-Hung Hsu. Increased sensitivity to Wnt/beta-catenin inhibitors in hepatocellular carcinoma cells harboring activating mutation of beta-catenin. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2625. doi:10.1158/1538-7445.AM2014-2625
Insulin Resistance and HIV-Related LipoatrophyInsulin Resistance and HIV-Related Lipoatrophy
Authors: Nasser Mikhail
Journal: JAMA (2002)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Rheumatic Fever Chemoprophylaxis and Penicillin-Resistant Gingival Organisms
Authors: William H. Spencer, Clyde Thornsberry, Max D. Moody, Nanette K. Wenger
Journal: Annals of Internal Medicine (1970)

Penicillin-resistant gingival organisms occur in patients on oral penicillin rheumatic fever chemoprophylaxis. Gingival organisms of 87 patients undergoing chemoprophylaxis were compared with those of controls not receiving chemoprophylaxis. Ten percent of patients receiving sulfadiazine prophylaxis, 42% receiving oral penicillin prophylaxis, 5% receiving benzathine penicillin prophylaxis, and 23% of controls had penicillin-resistant gingival organisms. Fourteen percent of the gingival flora of patients on oral penicillin prophylaxis were penicillin-resistant, a much greater percentage than that in the other three groups. Change of prophylaxis from sulfadiazine to oral penicillin caused an increase in penicillin-resistant organisms; when penicillin prophylaxis was discontinued and sulfadiazine prophylaxis resumed, these organisms decreased. Penicillin-resistant organisms included alpha streptococci, staphylococci, and micrococci; most were sensitive to erythromycin and lincomycin. Penicillin-resistant gingival organisms associated with oral penicillin prophylaxis may influence the choice of antibiotic for the prevention of bacterial endocarditis at the time of dental procedures.
Abstract 1283: Endothelial Nitric Oxide Synthase Promotes Mesenchymal Stem Cell Migration Towards the Ischemic Myocardium
Authors: Na Li, Fuli Xiang, Xiangru Lu, Morris Karmazyn, Qingping Feng
Journal: Circulation (2007)

Background: Bone marrow mesenchymal stem cells (MSCs) migrate from bone marrow towards the heart and contribute to cardiac repair post myocardial infarction. However, mechanisms by which MSCs migrate to the ischemic heart remain unclear. The present study investigated the role of endothelial nitric oxide synthase (eNOS) on MSC migration towards the ischemic myocardium and whether stromal cell derived factor-1 (SDF-1) contributes to the eNOS-mediated MSC migration. Methods and Results: MSCs were isolated from wild-type (WT) bone marrow and cultured in vitro for 3 generations. Coronary microvascular endothelial cells were isolated from adult mouse hearts and seeded on inserts for transendothelial migration assay. Neonatal cardiomyocytes were isolated from WT, eNOS −/− and eNOS transgenic (Tg) mice, cultured to subconfluence and subjected to 30 min of anoxia followed by 6 hours of reoxygenation (A/R). The conditioned medium was collected and served as a chemoattractant. MSC migration was significantly decreased in eNOS −/− compared to WT conditioned medium (9.8± 1.8% vs. 14.7±2.3%), but increased in eNOS-Tg conditioned medium (38.0±4.5%, P &lt;0.05). SDF-1 protein secretion was significantly decreased in eNOS −/− but increased in eNOS-Tg conditioned medium. To examine MSC migration in vivo, WT, eNOS −/− or eNOS-Tg mice were subjected to myocardial ischemia for 45 min followed by 24 hrs of reperfusion (I/R). Immediately after reperfusion, GFP + MSCs were administered via a tail vein. GFP + cells in the ischemic region were significantly decreased in eNOS −/− compared to WT hearts (3.4±0.3 vs. 5.6±0.4 cell per mm 2 , P&lt;0.05) but significantly increased in eNOS-Tg compared to WT (10.2±1.6 vs. 5.6±0.4 cell per mm 2 , P&lt;0.05). Furthermore, SDF-1 mRNA and protein expression was increased in eNOS-Tg as compared to WT and eNOS −/− after myocardial I/R. Conclusions: eNOS promotes MSC migration towards the ischemic myocardium. This is likely due to an upregulation of SDF-1.
Abstract 2754: Induction of Sp1 factor by CYP1B1 inhibits TRAIL-mediated apoptotic pathway
Authors: Yeo-Jung Kwon, Nahee Park, Sangyun Shin, Dong-Jin Ye, Mihye Hong, Young-Jin Chun
Journal: Cancer Research (2014)

Abstract Human cytochrome P450 1B1 (CYP1B1) belongs to the CYP1 family and is known as a major enzyme for estradiol 4-hydroxylation. It has been reported that CYP1B1 expression is higher in the tumor tissues than the normal ones, especially in hormone-related cancers such as breast, ovarian and prostate. Previously, we identified that CYP1B1 induces cell proliferation by activation of Wnt/β-catenin signal pathway. To explore the role of CYP1B1 on cancer cell proliferation further, we investigated whether CYP1B1 blocks apoptotic pathways. Using Western blot and RT-PCR, the expression of TRAIL was examined in MCF-7 cells, and we found that CYP1B1 overexpression or treatment with a CYP1B1 inducer, 7, 12-dimethylbenz[α]anthracene (DMBA) caused suppression of TRAIL and its receptors (DR4 and DR5) expression. However, when cells were treated with CYP1B1 siRNA or a selective CYP1B1 inhibitor, tetramethoxystilbene (TMS), expression of TRAIL and its receptors were significantly increased. Sp1, a zinc finger-containing transcription factor, has been reported to suppress TRAIL gene expression by binding TRAIL promoter. Interestingly, CYP1B1 overexpression and DMBA induced Sp1 expression and TMS was able to prevent DMBA-induced Sp1 expression. Sp1 knockdown with siRNA suppressed c-FLIP, an inhibitor protein of TRAIL-mediated apoptosis while it enhanced TRAIL and DR4 expression. These results were reversed by Sp1 overexpession. Moreover, attenuation of Sp1 DNA binding activity using mithramycin A, a Sp1 binding inhibitor, resulted in recovery of TRAIL expression suppressed by DMBA. Taken together, these data suggest that CYP1B1 prevents apoptosis via TRAIL suppression and this is mediated by Sp1 induction. Citation Format: Yeo-Jung Kwon, Nahee Park, Sangyun Shin, Dong-Jin Ye, Mihye Hong, Young-Jin Chun. Induction of Sp1 factor by CYP1B1 inhibits TRAIL-mediated apoptotic pathway. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2754. doi:10.1158/1538-7445.AM2014-2754
Abstract 20402: Exome Genotyping of Dallas Heart Study Reveals Putative Cardiac Disease-Causing Mutations: Disconnect Between Genotype and Phenotype
Authors: Douglas Stoller, Jonathan Cohen, Helen Hobbs
Journal: Circulation (2014)

Next generation sequencing has increased the scope and availability of exome sequencing to clinical practice. A major challenge of using the new technology is handling incidental findings. The American Society of Medical Genetics (ACMG) recommends that mutations in 56 genes, including 20 genes that cause heart disease, be reported to patients, regardless of the indication for sequencing. The implications of applying these recommendations to clinical practice are unclear. Here we screened participants of the Dallas Heart Study (n=4928), with the Illumina HumanExome BeadChip array. A total of 46 sequence variants in 6 of the ACMG cardiac genes were identified. Six pathogenic variants were found in 36 participants (0.7% of the cohort). Those with the putative pathologic variants in cardiac genes were not more likely to have a positive family history for cardiac disease or sudden death (69% vs 65%; p=0.81) or an elevated serum cardiac troponin (33% vs 22%; p=0.41] when compared to carriers of benign or low frequency variants of unknown significance (VUS) in the same genes (25 variants in 78 participants). No difference in median (interquartile range) of BNP [7.1 pg/ml (0.7-47 pg/ml) vs 4.9 pg/ml (0-20 pg/ml); p=0.34), ejection fraction [70% (63-76%) vs 71% (69-77%); p=0.41] or LV mass [151 g (115-190 g) vs 143 g (99-176 g); p=0.52] were identified between the two groups who had variants in GLA, MYBPC3, and TNNT2. The QTc interval was similar in those with putative pathogenic KCNQ1 variants and benign/VUS variants [416 ms (406-437 ms) vs 410 ms (401-427ms)]. Our data indicate that cardiac incidental findings will be identified in >1% of the population and that a significant proportion of these individuals will not have clinically significant heart disease.

   ![][1]  [1]: /embed/graphic-1.gif
Staphyloccocal scalded skin syndrome in healthy adults
Authors: G.K. Patel, S. Varma, A.Y. Finlay
Journal: British Journal of Dermatology (2000)

Journal Article Staphyloccocal scalded skin syndrome in healthy adults Get access G.K. Patel, G.K. Patel Department of Dermatology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, U.K. E‐mail: finlayay@cf.ac.uk Search for other works by this author on: Oxford Academic Google Scholar S. Varma, S. Varma Department of Dermatology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, U.K. E‐mail: finlayay@cf.ac.uk Search for other works by this author on: Oxford Academic Google Scholar A.Y. Finlay A.Y. Finlay Department of Dermatology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, U.K. E‐mail: finlayay@cf.ac.uk Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 142, Issue 6, 1 June 2000, Pages 1253–1255, https://doi.org/10.1046/j.1365-2133.2000.03571.x Published: 01 June 2000
Abstract 16783: Beta-Blockers and ACE Inhibitors Improve Long-Term Survival In Renal Transplant Recipients: Results From 10 Year Follow-Up in 321 Patients
Authors: Waqas Aftab, Shuja Rasool, Padmini Varadarajan, Ramdas G Pai
Journal: Circulation (2011)

Background: End stage renal disease is associated with a dismal cardiovascular prognosis and is improved to some extent by renal transplantation. Hence, we analyzed the cardiovascular predictors of survival after renal transplantation to gain insights into potential therapeutic targets to improve their outcomes. Methods: We abstracted the medical records of 321 consecutive patients from our institution who had received renal transplantation between 1995 and 2003. Survival was analyzed as a function of clinical and pharmacological variables. Results: The characteristics of the 321 patients were as follows: age at transplant 49+13 years, 40% males, HTN in 89%, diabetes mellitus (DM) in 37% and LV EF 58+17%. Over a follow up of 10+4 years, there were 119 deaths. Cox proportional hazard model showed DM (HR 1.5, P= 0.05), prior myocardial infarction (MI) (HR 2.6, P=0.001), perioperative MI (HR 2.6, P=0.002), and lack of beta blocker (BB) therapy (HR 1.6, P=0.06) and ACEI (HR 1.9, p=0.006) to be independent predictors of higher mortality. The 10 year survival in patients receiving BB was 80% vs. 63% for no BB and 82% for those receiving ACEI vs. 62% for no ACEI. Conclusions: (1) Annualized mortality after renal transplantation is about 3%, being higher in the aged, diabetics and those with prior or perioperative MI. (2) Use of BB and ACEI therapies are associated with a higher survival independent of CAD or DM indicating a potential protective role in this high risk population.
The human immunodeficiency virus type-1 central DNA flap is a crucial determinant for lentiviral vector nuclear import and gene transduction of human hematopoietic stem cells
Authors: Aude Sirven, Françoise Pflumio, Véronique Zennou, Monique Titeux, William Vainchenker, Laure Coulombel, Anne Dubart-Kupperschmitt, Pierre Charneau
Journal: Blood (2000)
Sneddon’s syndrome in a child
Authors: P. Gottlöber, G. Bezold, A. Schaer, W. Stolz, I. Friesecke, R.U. Peter, M. Kerscher
Journal: British Journal of Dermatology (2000)

S ir, Sneddon's syndrome was described by Sneddon in 1965. 1 Approximately 73% of patients with livedo racemosa show cerebrovascular damage with apoplexia, hemiplegia, epilepsy, psycho‐organic syndrome or dizziness. 2 Most of the patients are smokers or take oral contraceptives. Sneddon's syndrome most commonly affects women less than 40 years of age, 2 and is very rare in children or young adults. So far, only two patients younger than 18 years have been described. 3 We report a 16‐year‐old girl with livedo racemosa and cerebrovascular lesions with hemiparesis, in whom Sneddon's syndrome was diagnosed. A 16‐year‐old caucasian girl had a 5‐month history of reddish‐brown mottling of the skin on the lower legs. In winter, she developed deep ulcers surrounding the reddish streaks. Five months later, she suffered a prolonged reversible ischaemic neurological deficit with left hemiparesis. She did not smoke or take oral contraceptives. On admission, she presented with large, intensely reddish‐brown streaks with several deep, sharply demarcated, painful ulcers coated with yellow crusts, and some red nodules affecting predominantly the lower legs and the ankle region up to the thighs ( Fig. 1). On the left side where she had hemiparesis, finger–nose co‐ordination and diadochokinesis were pathological. Magnetic resonance imaging (MRI) of the head showed, in the T2w sequence, an area of 5 mm with increased signal intensity of the right crus cerebri as a sign of an acute infarction. 4 A hypertensive signal was also seen in the corresponding diffusion MRI. 4 Skin biopsy revealed a thrombotic occlusion of the very small subepidermal capillaries, with fibrin and erythrocytes in the small vessels. Cerebrospinal fluid parameters were normal, and duplex sonography of extracranial vessels did not show any pathological findings. The following parameters were normal or negative: blood cell count, coagulation profile (prothrombin, partial thromboplastin time, fibrinogen, antithrombin III, protein C and protein S), liver and kidney parameters including creatinine clearance, cholesterol, triglycerides, serum electrophoresis, β2‐microglobulin, complement C3 and C4, rheumatoid factor, antinuclear antibodies, cryoglobulins, cold agglutinins, lupus anticoagulant factors, anticardiolipin antibodies, cytoplasmic and perinuclear immunofluorescent pattern antineutrophilic cytoplasmic antibodies, factor V‐Leiden mutation, urine analysis, electrocardiogram, electroencephalogram and ophthalmological examination. The C‐reactive protein level was elevated.