tbuckley/cabot-search huggingface dataset:
Abstract 3417: Unselected Human Cardiosphere-derived Cells are Functionally Superior to c-Kit- or CD90-Purified Cardiosphere-derived Cells
Authors: Rachel Ruckdeschel Smith, Isotta Chimenti, Eduardo Marbán
Journal: Circulation (2008)

Cardiosphere-derived cells (CDCs), a naturally heterogeneous mixture of cell sub-populations, were grown from percutaneous endomyocardial adult human biopsy specimens (n=6). c-Kit + and CD90 + CDCs were selected using magnetic-activated cell sorting with excellent purity as determined by flow cytometry. Immunostaining revealed that ~30% of c-Kit + CDCs expressed Nkx2.5, ~100% of CD90 + CDCs expressed procollagen type I, and ~100% of both sub-populations expressed CD105. When placed in co-culture with neonatal myocytes and fibroblasts, c-Kit + CDCs expressed cardiac troponin I, while CD90 + CDCs expressed vimentin. In order to assess the therapeutic potential of purified CDCs, acute myocardial infarcts (MIs) were created in immunodeficient mice and c-Kit + (n=16), CD90 + (n=14), or CD105 + (n=3) CDCs were injected into the border zone. Echocardiograms were performed 3 weeks post-MI to measure left ventricular ejection fraction (LVEF). CD105-injected mice were comparable to an historical control group of mixed CDC-injected mice (LVEF = 41.3±2.9% CD105 vs. 42.8±10.4% CDC [n=11], p=0.60), indicating that the sorting process did not itself impair the therapeutic potential of CDCs. c-Kit- and CD90-injected mice were indistinguishable from one another (LVEF=31.7±8.2% c-Kit vs. 32.1±11.8% CD90, p=0.92), and both groups were significantly outperformed by the CD105-injected mice (p=0.01 and p=0.03, respectively). All groups were then compared to two other historical control groups, mice treated with normal human dermal fibroblasts (NHDFs [n=7]) and mice treated with phosphate-buffered saline (PBS [n=11]). c-Kit-injected mice did significantly outperform both NHDF- (p=0.04) and PBS-injected mice (p=0.03), while more variability in the CD90-injected group resulted in nearly significant comparisons with the NHDF (p=0.08) and PBS groups (p=0.08). While the therapeutic mechanisms of action of these two distinct sub-populations are undoubtedly different, both offer similar global functional benefits in the setting of acute MI. We conclude that the spontaneously-emerging unselected CDC population serves as a therapeutic cell cocktail, and that no functional advantage is conferred by the extra step of sorting for c-Kit + or CD90 + sub-populations.
Abstract 2546: Periprocedural Stroke in Patients Undergoing Catheter Ablation of Atrial Fibrillation: The Impact of Periprocedural Thereapeutic INR
Authors: Luigi Di Biase, J. David Burkhardt, Rodney Horton, Javier Sanchez, G. Joseph Gallinghouse, Shane Bailey, Prasant Mohanty, Dimpi Patel, Jennifer E. Cummings, Robert A. Schweikert, Dhanunjay Lakkireddy, Atul Verma, Steven Hao, Richard Hongo, Salwa Beheiry, Amin Al-Ahmad, Paul Wang, Antonio Dello Russo, Gemma Pelargonio, Michela Casella, William R. Lewis, Aldo Bonso, Sakis Themistoclakis, Antonio Raviele, Ospedale Dell'Angelo, Andrea Natale
Journal: Circulation (2009)
Abstract 2173: Effect of High-dose Atorvastatin on Changes in Renal Function: A Secondary Analysis of the Stroke Prevention by Aggressive Reduction of Cholesterol Levels (SPARCL) Trial
Authors: Vito M Campese, Alfred Callahan, Amy E Rudolph, Michael Messig, Pierre Amarenco, Larry B Goldstein, Michael Hennerici, Henrik Sillesen, Justin A Zivin, K M Welch
Journal: Circulation (2007)
Abstract 683: Regression of Plaque Volume in One Arterial Bed is not Necessarily Associated with Similar Changes in Other Vessels
Authors: Takayuki Suzuki, Takashi Nozawa, Mitsuo Sobajima, Takashi Ohori, Akira Matsuki, Nozomu Fujii, Akihiko Igawa, Tomoki Kameyama, Hiroshi Inoue
Journal: Circulation (2008)

Background: Population-based studies have shown good correlation between severity of atherosclerotic disease in one arterial bed and involvement of other vessels. However, in patients with coronary artery disease (CAD), it remains unclear whether atherosclerotic plaque in an artery might regress or progress in parallel with other vessels. Accordingly, the present study was performed in patients with CAD to compare changes in plaque volume (PV) between the left main (LMT) and right coronary arteries (RCA), thoracic descending aorta (TDA) and common carotid artery (CCA), and to clarify clinical factors and biomarkers which might affect changes in PV in each artery. Methods: Using 64-multislice computed tomography, PVs in each artery were determined before and after 2.0-year follow-up period in 52 patients with CAD (67.4±9.9yo). Based on our previous study using ultrasound, CCA-PV was determined at windows of 90–240HU and TDA-PV determined manually. Coronary soft plaque was determined at windows of 0–75HU. Plasma levels of hsCRP, matrix metalloproteinase (MMP)-9 and urinary 8-iso-prostaglandin F2 α (PGF) were determined at baseline. Results: At baseline, PVs of TDA were correlated with CCA-PV (r=0.38, p&lt;0.02), but PVs of other arteries did not correlate to each other. Two-year later, PVs of LMT, RCA, TDA, and CCA were reduced in 41, 62, 27, and 39% of patients, respectively. Changes in LMT-PV were weakly related with those of TDA-PV (r=0.37, p=0.02) and RCA-PV (r=0.31, p=0.08), but there were no relation between other arteries. The multivariate analysis revealed that treatment with statin and low LDL-cholesterol (C, &lt;100mg/dl) were independent variables regarding a reduction in DTA-PV, but, in LMT, only low LDL-C was independent variable. However, there were no independent variables in RCA or CCA. The ratio of soft PV to total PV was similar between LMT (45.2±7.1%) and RCA (45.7±4.9%) at baseline and was unchanged in the follow-up study. None of hsCRP, MMP-9 or PGF levels was related with PVs of any arteries at baseline and with changes in PVs. Conclusions: Regression of PV in one arterial bed dose not necessarily allow us to predict atherosclerotic changes in the other vessels. Major factors which affect changes in PV may not be homogeneous between arteries.
E-cadherin promoter region methylation and its correlation with transcript level for the NCI-60 cancer cell lines
Authors: William C. Reinhold, Sohyoung Kim, Claudia Stewart, Samir Lababidi, Alika Maunakea, Micah Ziegler, Satoshi Nishizuka, Hengmi Cui, Kimberly Bussey, Yves Pommier, David Munroe, Andrew Feinberg, John N. Weinstein
Journal: Cancer Research (2004)

4984  We are mapping the methylation patterns of cancer interesting genes in the NCI-60 cell lines using-high throughput “bisulfite-sequencing”. The NCI-60 cell lines represent nine tissues of origin, including both epithelial and non-epithelial types. Candidate genes were selected on the basis of documented importance in cancer and the occurrence of functionally significant promoter region methylation. The first of these genes for which the studies have been completed is E-cadherin. E-cadherin is a transmembrane glycoprotein whose decreased expression in many tumors of epithelial origin is associated with tumor invasion and metastasis. Here, we report detailed CpG island methylation patterns for the promoter region of E-cadherin. Included in the region amplified by nested PCR for sequencing was a 337-base stretch that included 29 CpG cytosines. When analyzed by tissue of origin, the mean methylation levels across all 29 CpG sites were 42% for breast, 18% for colon, 28% for non-small cell lung, 26% for ovarian, 18% for prostate, 58% for renal, 61% for central nervous system, 84% for leukemia, and 47% for melanoma. The methylation patterns were visualized and analyzed using MethMiner software (S. Kim, manuscript in preparation). To examine in detail the relationship between CpG island methylation and gene expression, we compared the methylation profile of the NCI60 with transcript expression data from four different experimental platforms: pin-spotted cDNA microarrays (Incyte, Palo Alto, CA), and HU6800, U95, and U133 Affymetrix oligonucleotide chips (U95 and U133 done by GeneLogic, Gaithersburg, MD). The Pearson’s correlation coefficients for these microarrays, with Bootstrap resampling to establish confidence intervals, were statistically significant at the P=0.05 level, with values of –0.55, -0.53, -0.57, and –0.62, respectively. The negative correlation indicates that an increase in methylation is associated with a decrease in transcript level that is a relative silencing of the gene.
Abstract 5957: Randomized Comparison of Bivalirudin Versus Tirofiban Plus Unfractionated Heparin Administration in Diabetic Patients Undergoing Elective Coronary Stenting
Authors: Davide Tavano, Gabriella Visconti, Davide D'Andrea, Amelia Focaccio, Maria Teresa Librera, Bruno Golia, Bruno Ricciardelli, Carlo Briguori
Journal: Circulation (2008)
Abstract 1565: Menage-A-Trois 1 Physically Associates with PGC-1 and is Essential for Cardiac Mitochondrial Function
Authors: Yasukatsu Izumi, Motoaki Sano, Christopher R Wilson, Fernando Scaglia, Masanori Asakura, Katja Helenius, Derrick J Rossi, William J Craigen, Anastasia Kralli, Noriaki Shumizu, Hiroshi Tanaka, Heinrich Taegtmeyer, Tomi P MA{curren}kelA{curren}, Michael D Schneider
Journal: Circulation (2006)
Abstract 4453: Successful Treatment of Clopidogrel Hypersensitivity Without Drug Interruption
Authors: Kimberly L Campbell, Raghuram Mallya, Waseem Jaffrani, John R Cohn, David L Fischman, Paul Walinsky, Michael P Savage
Journal: Circulation (2008)

Premature discontinuation of dual antiplatelet therapy is an important predictor of stent thrombosis. Hypersensitivity affects 6% of patients treated with clopidogrel often resulting in drug discontinuation. Clopidogrel desensitization has been previously described utilizing a protocol of dose titration following a washout period to enable detection of reaction to desensitizing dose. This technique is problematic in patients with recently implanted stents since therapy interruption carries significant risk of stent thrombosis. The purpose of this study was to assess a strategy of clopidogrel hypersensitivity management without drug interruption utilizing corticosteroids and antihistamines to allow for development of physiologic tolerance. A retrospective analysis of clopidogrel hypersensitivity following stent placement was performed in patients treated from January 1, 2005 to April 30, 2008. The primary outcome was response of hypersensitivity symptoms to treatment regimen. Secondary outcomes included duration of clopidogrel therapy and recurrence of hypersensitivity reaction. The study population consisted of 20 patients (15 men and 5 women) with mean age 62 ± 9 years who underwent PCI and subsequently developed clopidogrel hypersensitivity at mean 6 ± 2 days of therapy. Indications for revascularization included STEMI (14%), acute coronary syndrome (43%), stable angina (5%), and positive stress test (38%). Patients received a mean of 2.2 stents, with drug eluting stents in 16 cases (76%). Treatment of clopidogrel hypersensitivity was successful in 17 of 20 patients (85%). Treatment regimens included corticosteroids (15) and antihistamines (17) with combination therapy in 11. Median duration of corticosteroid therapy was 6 days (range 6 –20). Mean duration of clopidogrel therapy was 257 ± 287 days with a median of 151 days. Two patients required repeat course of corticosteroids for relapse. Only 3 patients prematurely discontinued clopidogrel therapy due to persistent symptoms. In conclusion, patients who develop clopidogrel hypersensitivity after stent placement can be successfully treated with short-course corticosteroids and antihistamines without interruption of dual antiplatelet therapy.
Recovery from Acute Radiation Injury in Mice Following Administration of Rat Bone Marrow&lt;xref ref-type="fn" rid="fn1"&gt;2&lt;/xref&gt;
Authors: Leonard J. Cole, John G. Habermeyer, Victor P. Bond
Journal: JNCI Journal of the National Cancer Institute (1955)

Recovery from Acute Radiation Injury in Mice Following Administration of Rat Bone Marrow Get access Leonard J. Cole, Leonard J. Cole Division of Biological and Medical Sciences, U. S. Naval Radiological Defense Laboratory, San Francisco, Calif. Search for other works by this author on: Oxford Academic PubMed Google Scholar John G. Habermeyer, John G. Habermeyer Division of Biological and Medical Sciences, U. S. Naval Radiological Defense Laboratory, San Francisco, Calif. Search for other works by this author on: Oxford Academic PubMed Google Scholar Victor P. Bond Victor P. Bond Division of Biological and Medical Sciences, U. S. Naval Radiological Defense Laboratory, San Francisco, Calif. Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 16, Issue 1, August 1955, Pages 1–9, https://doi.org/10.1093/jnci/16.1.1 Published: 01 August 1955 Article history Received: 22 November 1954 Published: 01 August 1955
Detection of influenza A(H3N2) virus in children with suspected mumps during winter 2014/15 in England
Authors: C I Thompson, J Ellis, M Galiano, M Ramsay, K E Brown, M Zambon
Journal: Eurosurveillance (2015)

Influenza A(H3N2) virus was detected in oral fluid from 16/107 children (aged 2 to 12 years) with a clinical diagnosis of mumps, who were sampled between December 2014 and February 2015 in England, during the peak of the 2014/15 influenza season. Sequence analysis of an A(H3N2) virus from a child with suspected mumps showed the virus was similar to other circulating A(H3N2) viruses detected in winter 2014/15, which were antigenically drifted from the A(H3N2) vaccine strain.
Development of a topical ointment of betamethasone dipropionate loaded nanostructured lipid carrier
Authors: Xin Kong, Yuan Zhao, Peng Quan, Liang Fang
Journal: Asian Journal of Pharmaceutical Sciences (2015)

The purpose of this study was to design an innovative topical ointment containing betamethasone dipropionate loaded nanostructured lipid carrier (BD-NLC) for the treatment of atopic dermatitis (AD). BD-loaded NLC was produced with precirol ATO 5 and oleic oil (OA) by melt emulsification method. Effects of surfactant concentration, amount of solid lipid and liquid lipid on skin retention and skin penetration were investigated by in vitro percutaneous permeation experiment. The optimized BD-NLC showed a homogeneous particle size of 169.1 nm (with PI = 0.195), negatively charged surface (−23.4 mV) and high encapsulation efficiency (85%). Particle morphology assessed by TEM revealed a spherical shape. In vitro skin permeation study was carried out to investigate the percutaneous behaviors of W/O ointment with BD-NLC and Carbopol emulgel ointment with BD-NLC. W/O ointment with BD-NLC showed high skin retention (35.43 µg/g) and low penetration (0.87 µg/ml). In vitro drug release studies were carried out to demonstrate the drug releasing properties of the two ointments. W/O ointment with BD-NLC showed an advantage for skin retention as it was better for drug release. The tissue distribution test suggested that BD distribution was skin > muscle > blood. Self-made topical ointment in mice showed no skin irritation. The animal experiments indicated that BD-loaded NLC ointment was effective and safe for topical use.
Abstract 17001: Net Clinical Benefits of Ticagrelor Compared With Clopidogrel in Asian Acute Coronary Syndrome Patients: A Plato Sub-Study
Authors: Hyun-Jae Kang, Robert M Clare, Runlin Gao, Anders Himmelmann, Claes Held, Lars Wallentin, Stefan James, Richard C Becker
Journal: Circulation (2013)

Background: In the PLATO study, ticagrelor was superior to clopidogrel in reducing cardiovascular events among patients with acute coronary syndrome (ACS). Because Asian patients, when compared wit...
Abstract 2897: Targeting Engraftment of Endothelial Progenitor Cells to Ischemic Tissue by Ultrasound-mediated Transfection of Stromal Cell Derived Factor-1
Authors: Michael A Kuliszewski, Jeremy Kobulnik, Jonathan R Lindner, Duncan J Stewart, Howard Leong-Poi
Journal: Circulation (2007)

The homing of endothelial progenitor cells (EPC) to sites of ischemic injury is critical to successful neovascularization. We hypothesize that ultrasound-mediated (UM) transfection of stromal-cell derived factor-1 (SDF-1) would enhance engraftment of exogenously administered EPCs and improve neovascularization in a rat model of chronic hindlimb ischemia. Methods: Bone-marrow derived EPCs were isolated from donor F344 rats, cultured for 7 days, labelled with CMTMR and injected (1 x 10 6 ) into recipient animals via a jugular vein. Unilateral hindlimb ischemia was created by iliac artery ligation in 32 rats. At day 14 post ligation, microvascular blood volume (MBV) and blood flow (MBF) in the hindlimb muscles were assessed by contrast-enhanced ultrasound (CEU). Rats were assigned to 4 treatment groups: control untreated animals, EPCs alone, UM delivery of SDF-1 plasmid (500 μg DNA + 1x10 9 cationic microbubbles), and both UM delivery of SDF-1 + EPCs (n=8 per group). Perfusion was re-assessed at day 28. EPC engraftment was assessed using fluorescent microscopy. Circulating exogenous EPC numbers were determined by FACS at various time-points post injection. Results: Prior to EPC delivery, normalized MBV and MBF in ischemic muscle were similarly reduced in all groups. Control animals showed no changes in perfusion over time. By day 28, EPC treated animals had a significant increase in normalized MBV (1.04±0.24 vs 0.67±0.22, p&lt;0.001), without significant improvements in MBF (0.44±0.13 vs 0.33±0.15, p=NS), while SDF-1/EPC treated animals had significant improvement in both normalized MBV (1.33±0.51 vs 0.67±0.22, p&lt;0.001), and MBF (0.55±0.26 vs 0.33±0.15, p&lt;0.001). Maximal exogenous circulating EPCs could be detected at 72 hours post injection. Fluorescent microscopy showed little to no CMTMR labelled cells in the non-ischemic hindlimb in all groups. In comparison, a rich population of cells was found in the ischemic hindlimb of both the SDF-1/EPC and EPC treated animals, with the SDF-1/EPC animals demonstrating the greatest density of EPCs. Conclusions: Ultrasound-mediated transfection of SDF-1 improves intravenous EPC targeting, enhancing EPC engraftment into chronically ischemic tissue and leading to greater neovascularization response.
GOUT, MYXEDEMA, AND HYPEROSTOSIS FRONTALIS INTERNA
Authors: Daniel H. Goodman
Journal: JAMA (1960)

Gout in a Negro woman is rare; there are only four reports in the literature.<sup>1</sup>Hyperostosis frontalis interna (Morgagni-Stewart-Morel syndrome)<sup>2</sup>is not uncommon and is associated with obesity; hypertension; and nervous, metabolic, and endocrine disturbances. Myxedema,<sup>3</sup>as well as gout,<sup>4</sup>has been previously reported in association with hyperostosis frontalis interna. However, the unique combination of gout, myxedema, and the classical features of hyperostosis frontalis interna in a Negro woman has never been recorded. A 66-year-old Negro woman was admitted to the Maricopa County General Hospital with complaints of pains in her knees, ankles, hips, and the great toes of both feet of eight years' duration. In addition, she complained of puffiness of both eyelids, swelling of the face, intolerance to cold, and dryness of the skin. Her hair became brittle and began to fall out. She complained of frontal and occipital headaches, nervousness, constipation, fainting spells,
Phenomenology and course of psychiatric disorders associated with combat-related posttraumatic stress disorder
Authors: T A Mellman, C A Randolph, O Brawman-Mintzer, L P Flores, F J Milanes
Journal: American Journal of Psychiatry (1992)

Studies indicate that chronic combat-related posttraumatic stress disorder (PTSD) is frequently associated with other psychiatric disorders. Questions regarding the nature and interrelationships of these conditions require clarification. The purpose of this study was to address primary and secondary illness relationships by focusing on the specific phenomenology and course of illness onset of PTSD comorbidity.In order to minimize confounding factors, only outpatients without recent substance use disorders were included. Sixty subjects who had been exposed to severe combat stress including veterans of Vietnam and veterans of World War II or Korea, 15 of whom were former prisoners of war, received structured assessments over serial evaluations.PTSD was the most prevalent lifetime disorder followed by major depression, panic disorder, generalized anxiety disorder, and phobic disorder or symptoms. Endogenous-appearing features overlapping other clinical populations were common; however, some specific symptom patterns also were suggestive of traumatic influence. Unlike generalized anxiety disorder and past substance use, the mean onset of phobias, major depression, and panic disorder, respectively, occurred later than PTSD.These observations suggest that persistent conditions related to PTSD progress toward symptoms that are increasingly autonomous in their pattern of occurrence.
Preventive and therapeutic treatment with a7 nicotinic receptor agonist reduced lung inflammation and collagen deposition in mice with acute lung injury
Authors: Nathalia Pinheiro, Fernanda Santana, Marina Guerreiro, Luciana Caperuto, Iolanda Tiberio, Vânia Prado, Marco Antonio Prado, Milton Martins, Carla Prado
Journal: European Respiratory Journal (2014)

Although cholinergic activity is involved in airway tone control, particularly by stimulation of muscarinic receptors, there is evidence that cholinergic anti-inflammatory pathway modulates immune systemic inflammatory responses in different models. Aim:  We evaluated whether a7-nicotinic acetylcholine receptor (a7nAChR)stimulation reverted lung inflammation observed in an experimental model of acute lung injury (ALI). Methods: Male C57/BL6 mice received PNU-282987 (specificα7nAChRagonist) 30 min before (10 mg/kg, ip) or 6 hours after (2.5; 5 or 10 mg/kg) LPS administration (5 mg/kg, intratraqueal). We evaluated pulmonary mechanics, bronchoalveolar lavage fluid (BALF), collagen fibers and activation of NF-kBby immunohistochemistry and by Western Blotafter 24 hours from LPS. Results: After six hours, LPS treated animals presented increase in neutrophils and macrophages in BALF. Both pre and pos-treatment with PNU (10mg/Kg) reduced the total cells, macrophagesand neutrophilsin BALF (p Conclusion:  We concluded that a7 nAChRstimulation prevents and revertslung inflammation in a model of ALI probably by controlling NF-kB activation. Our data reinforced the idea that a7 nAChRis an important pathway to be better explored in the treatment of acute respiratory distress syndrome.
Defective Apoptotic Signal Transduction Pathway Downstream of Caspase-3 in Human B-Lymphoma Cells: A Novel Mechanism of Nuclear Apoptosis Resistance
Authors: Yoshinari Kawabata, Makoto Hirokawa, Atsushi Kitabayashi, Takahiro Horiuchi, Jun Kuroki, Akira B. Miura
Journal: Blood (1999)