tbuckley/cabot-search huggingface dataset:
Comparison of cyclic adenosine 3':5'-monophosphate and cyclic guanosine 3':5'-monophosphate levels, cyclases, and phosphodiesterases in Morris hepatomas and liver.
Authors: Hickie Ra, Thompson Wj, Strada Sj, Couture-Murillo B, Morris Hp, Robison Ga
Journal: Cancer Research (1977)

As a test of the hypothesis that cell proliferation is regulated by opposing effects of cyclic adenosine 3′:5′-monophosphate (cAMP) and cyclic guanosine 3′:5′-monophosphate (cGMP), the levels of these cyclic nucleotides were investigated in a rapidly growing hepatoma (7288ctc), two intermediate-growth-rate tumors [5123tc (h) and 5123C], one slow-growing hepatoma (7794A), host liver, and normal liver. Changes in cyclic nucleotide levels were also compared to alterations in activities of the soluble and particulate cyclases and phosphodiesterases in these tissues.

In hepatoma 7288ctc the cAMP content was slightly lower than that in normal liver (12%), whereas the cGMP levels were more than 4-fold higher. The cAMP levels of 5123tc (h) and 5123C were 20 to 27% lower, whereas the cGMP levels were 25 to 33% higher than those of normal liver. For 7794A the cAMP content was 42% higher than that in normal liver, whereas the cGMP levels were 75% higher. In host livers the cAMP levels were usually higher than those in normal liver (22 to 47%) in animals bearing the rapidly growing and intermediate-growth-rate hepatomas; host liver cGMP levels also tended to be 17 to 33% higher than those of normal liver. Although the cGMP/cAMP ratios are generally higher in hepatomas than they are in normal liver, there is no direct correlation between tumor growth rate and cyclic nucleotide content or the corresponding cGMP/cAMP ratios.

Changes in enzyme activities common to all four hepatomas included reduced activities of the total particulate cAMP phosphodiesterases as well as depressed activities of soluble guanylyl cyclase and particulate cGMP phosphodiesterase. The responsiveness of adenylyl cyclase to glucagon was impaired to a greater degree in the rapid- and intermediate-growth-rate tumors than it was in the slow-growing one; this observation confirms previous findings of other workers on different hepatomas. The total activities of the apparent high-affinity (low-Km) cAMP phosphodiesterase were substantially lower than those of normal liver in 7288ctc, 5123C, and 7794A but were similar to those of liver in 5123tc (h); some of the particulate fractions of the latter tumor actually had specific activities that exceeded those of normal liver. It is concluded that the cGMP/cAMP ratios observed in this study coincide more closely with specific changes in activities of the cyclases and/or phosphodiesterases than with differences in hepatoma growth rate.
脑外伤的评估、检查和早期处理：英国国家卫生和临床医疗优选研究所指南概要
Authors: David, Yates, Rifna, Aktar, Jennifer, Hill, 魏妍平, 高晶
Journal: BMJ (2008)

无论从逻辑上抑或临床上，脑外伤都是一个颇为重要的公众健康问题。尽管只有少数患者需要在神经医学中心进行诊治，但是许多患者需要给予他们相关的医疗建议。大多数患者恢复良好，但致残率非常高，即使是轻微外伤后。
Decade of Aortic Valve Sparing Reimplantation
Authors: Klaus Kallenbach, Matthias Karck, Dorota Pak, Rolf Salcher, Nawid Khaladj, Rainer Leyh, Christian Hagl, Axel Haverich
Journal: Circulation (2005)

Background— This single center study assesses the outcome of aortic valve sparing reimplantation (AVS) in 284 consecutive patients who were operated on for various indications during the last 11 years. Methods and Results— From July, 1993, to July, 2004, 284 patients underwent AVS. Mean age was 53±16 (range 8 to 84) years. Of the 284 patients, 184 were male (64.8%) and 54 (19%) experienced Marfan’s syndrome. Acute aortic dissection Stanford type A was present in 53 patients (19%) and a bicuspid aortic valve was present in 17 patients (6%). Concomitant arch replacement was necessary in 120 patients (42%). Additional procedures were performed in 66 patients (23.2%). Mean follow-up time was 41±32 (range 0 to 130) months. The 30-day mortality was 3.2% overall, 11.3% in emergency patients, and 1.3% in elective patients. Mean bypass time was 174±48 (range 90 to 440) minutes and aortic cross clamp time was 132±33 (range 64 to 283) minutes. In patients undergoing arch replacement, circulatory arrest was 25±17 (range 7 to 99) minutes. Rethoracotomy for bleeding was required in 4.6% of patients. During follow-up, there were 20 (7.3%) late deaths. Reoperation of the reconstructed valve was required in 15 patients (5.3%); underlying reasons were endocarditis (n=4) and aortic insufficiency (n=11) requiring aortic valve replacement. Average grade of aortic insufficiency increased significantly from 0.23±0.46 postoperatively to 0.34±0.59 at latest evaluation ( P =0.026). Two patients experienced a transient ischemic attack early postoperatively; no further thromboembolic complications were noticed. The majority of patients (96%) presented with a favorable exercise tolerance at last contact. Conclusions— The aortic valve reimplantation technique leads to excellent clinical outcome in patients with various pathologies. Lack of anticoagulation and favorable durability should encourage the extension of indications for this technique.
Abstract 3950: Integrative analysis of acute and late radiotherapy effects using genome-wide transcriptomics and quantitative proteomics
Authors: Tilmann Rackwitz, Philipp Seidel, Martin Winter, Cheng Zhou, Ramona Mayer, Uwe Warnken, Peter Hofner, Jürgen Debus, Martina Schnölzer, Amir Abdollahi
Journal: Cancer Research (2014)

Abstract Pulmonary microvascular endothelial cells are critically involved in development of radiation-induced acute inflammation and late fibrosis often limiting the delivery of sufficient dose to optimally treat lung cancer. Moreover, tumor-endothelium communication has emerged as a key target to augment radiotherapy by resensitizing endothelial-specific survival signals. Therefore, we aimed to employ high-throughput genome-wide gene expression and whole proteome analysis to uncover the molecular mechanisms governing tumor-vessel communication and radiation-induced normal tissue toxicity. Human primary isolated pulmonary microvascular endothelial cells (HPMEC) were irradiated with 0 or 6Gy. Cells were harvested two hours (acute) and six days (late) after irradiation and proteins as well as RNA were extracted. Gene expression analysis was performed using 47k Illumina microarray (HumanHT-12 v4) platform. Mass spectrometry on an LTQ-Orbitrap instrument was employed for protein identification and relative quantification using label-free quantification techniques. An IMAC/TiO2 enrichment step to obtain phosphorylated peptides was carried out and analyzed by mass spectrometry as well. The regulation of candidate radiation-regulated genes and proteins were confirmed in-vitro and in irradiated lung specimen (C57BL/6 mouse) by real-time qRT-PCR, western blot and ELISA. At the early timepoint a strong induction of genes involved in apoptosis and DNA-damage response such as GADD45A, PARP3 and DDB2 was identified. In contrast to the acute radiation response, cell adhesion (Integrins), pro-inflammatory (Interleukines), pro-angiogenic and matrix-remodeling related genes (MMPs, PDGFs, VEGFs) were induced late after irradiation. Integrative analysis of protein quantification, phosphorylation and gene-expression further revealed novel insights to an intricate gene/protein regulatory network underlying late radiation response in microvascular endothelium. The here identified genes and proteins involved in late radiation response constitute attractive targets for modulation of radiotherapy effects in tumor-endothelium or prevention of undesired radiotherapy-induced late side effects. Citation Format: Tilmann Rackwitz, Philipp Seidel, Martin Winter, Cheng Zhou, Ramona Mayer, Uwe Warnken, Peter Hofner, Jürgen Debus, Martina Schnölzer, Amir Abdollahi. Integrative analysis of acute and late radiotherapy effects using genome-wide transcriptomics and quantitative proteomics. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3950. doi:10.1158/1538-7445.AM2014-3950
The origin of IgG production and homogeneous IgG components after allogeneic bone marrow transplantation
Authors: MJ van Tol, EJ Gerritsen, GG de Lange, AM van Leeuwen, CM Jol-van der Zijde, NJ Oudeman-Gruber, E de Vries, J Radl, JM Vossen
Journal: Blood (1996)
Spectrum of enteropathogens detected by the FilmArray GI Panel in a multicentre study of community-acquired gastroenteritis
Authors: A Spina, K G Kerr, M Cormican, F Barbut, A Eigentler, L Zerva, P Tassios, G A Popescu, A Rafila, E Eerola, J Batista, M Maass, R Aschbacher, K E P Olsen, F Allerberger
Journal: Clinical Microbiology and Infection (2015)

The European, multicentre, quarterly point-prevalence study of community-acquired diarrhoea (EUCODI) analysed stool samples received at ten participating clinical microbiology laboratories (Austria, Finland, France, Germany, Greece, Ireland, Italy, Portugal, Romania, and the UK) in 2014. On four specified days, each local laboratory submitted samples from ≤20 consecutive patients to the Austrian Study Centre for further testing with the FilmArray GI Panel (BioFire Diagnostics, Salt Lake City, UT, USA). Of the 709 samples from as many patients received, 325 (45.8%) tested negative, 268 (37.8%) yielded only one organism, and 116 (16.4%) yielded multiple organisms. Positivity rates ranged from 41% (30 of 73 samples) in France to 74% (59 of 80 samples) in Romania. With the exception of Entamoeba histolytica and Vibrio cholerae, all of the 22 targeted pathogens were detected at least once. Enteropathogenic Escherichia coli, Campylobacter species, toxigenic Clostridium difficile, enteroaggregative E. coli, norovirus and enterotoxigenic E. coli were the six most commonly detected pathogens. When tested according to local protocols, seven of 128 positive samples (5.5%) yielded multiple organisms. Overall, the FilmArray GI Panel detected at least one organism in 54.2% (384/709) of the samples, as compared with 18.1% (128/709) when testing was performed with conventional techniques locally. This underlines the considerable potential of multiplex PCR to improve routine stool diagnostics in community-acquired diarrhoea. Classic culture methods directed at the isolation of specific pathogens are increasingly becoming second-line tools, being deployed when rapid molecular tests give positive results. This optimizes the yield from stool examinations and dramatically improves the timeliness of diagnosis.
Practical Events in the Management of a Collodion Baby
Authors: Michelle A. Nguyen, Ari Gelman, Scott A. Norton
Journal: JAMA Dermatology (2015)

In our series, all patients had quick good-quality remission with a median progression-free survival of 6 months.The rapidity of the response supports the role of vinblastine instead of spontaneous regression in these patients with multifocal lesions and a long-term history of refractory disease.In our series, no patient had to discontinue use of the drug because of infection or severe toxic effects.Vinblastine should be studied in larger series to confirm its effectiveness and tolerance in disseminated or refractory C-ALCL.Its place as second-line treatment could be considered with failure of methotrexate and perhaps as first-line treatment in the case of contraindication to methotrexate, especially in older adults.
The role of stem cell factor and c-KIT in keloid pathogenesis: do tyrosine kinase inhibitors have a potential therapeutic role?
Authors: A. Mukhopadhyay, D.V. Do, C.T. Ong, Y.T. Khoo, J. Masilamani, S.Y. Chan, A.S. Vincent, P.K. Wong, C.P. Lim, X. Cao, I.J. Lim, T.T. Phan
Journal: British Journal of Dermatology (2010)

Background Keloids are fibroproliferative disorders characterized by increased deposition of extracellular matrix components. Stem cell factor (SCF) and its receptor c-KIT are expressed in a wide variety of cells and have also been demonstrated to be important modulators of the wound healing process. Objectives To examine the role of the SCF/c-KIT system in keloid pathogenesis. Methods Immunohistochemical staining and Western blot analyses were used to examine localization and expression of SCF and c-KIT in keloid and normal skin tissue. This was followed by the detection of SCF and c-KIT expression in fibroblasts cultured in vitro and fibroblasts exposed to serum. To investigate the effect of epithelial–mesenchymal interactions, a two-chamber system was employed in which keratinocytes on membrane inserts were cocultured with the fibroblasts. SCF and c-KIT expression levels in all cell extracts and conditioned media were assayed by Western blotting. In another set of experiments, the effect of imatinib (Glivec®, Gleevec®; Novartis Pharma AG, Basel, Switzerland) on keloid fibroblasts was examined. Results SCF and c-KIT were upregulated in keloid scar tissue and in cultured fibroblasts stimulated with serum, highlighting their importance in the initial phase of wound healing. We further demonstrated that epithelial–mesenchymal interactions, mimicked by coculture of keratinocytes and fibroblasts in vitro, not only stimulated secretion of the soluble form of SCF in keloid cocultures but also brought about shedding of the extracellular domain of c-KIT perhaps by upregulation of tumour necrosis factor-α converting enzyme which was also upregulated in keloid scars in vivo and keloid cocultures in vitro. In addition keloid cocultures expressed increased levels of phosphorylated c-KIT highlighting an activation of the SCF/c-KIT system. Finally, we demonstrated that imatinib, a tyrosine kinase inhibitor, may be a possible therapeutic agent for keloids. Conclusion These data indicate that the SCF/c-KIT system plays an important role in scar pathogenesis, and underscore the role of imatinib as a key therapeutic agent in keloid scars.
891. Effect of the Timing of Direct Adenoviral Gene Delivery on the Healing of Critical Sized Bone Defects in Rats
Authors: Oliver B. Betz, Marcus Egermann, Volker M. Betz, Ara Nazarian, Andre F. Steinert, Glyn D. Palmer, Mary L. Bouxsein, Mark S. Vrahas, Thomas A. Einhorn, Christopher H. Evans
Journal: Molecular Therapy (2004)
Abstract 20076: Hybrid Approach for Ablation of Atrial Fibrillation - A Systematic Review and Meta-analysis
Authors: Jason A Varzaly, Dennis H Lau, Anthony Brooks, James Edwards, Robert Stuklis, Michael Worthington, Prashanthan Sanders
Journal: Circulation (2014)

Introduction: Catheter ablation has been increasingly utilized as an effective rhythm control strategy for atrial fibrillation (AF); although, the durability of results has been variable. The hybrid approach (joint surgical and catheter ablation) is an emerging technique with several studies demonstrating promising results. Methods: A systematic literature review was conducted to March 2013 using PUBMED/EMBASE. Broad search terms included “Cox-Maze, Mini-Maze, ablation methods (including RF, cryoablation, cryomaze), surgery and hybrid”. This yielded 3637 results with abstracts reviewed to remove studies that did not pertain to hybrid ablation. A total of 116 full-text articles were assessed with 9 suitable studies included for analysis. Results: The 9 included studies comprised of 6 case series and 3 prospective studies with a mean follow up of 17±9 months and overall cohort of 271 patients (17% paroxysmal, 24% persistent & 59% longstanding persistent). All studies utilized radiofrequency as the energy so...
ABC of intensive care: Recovery from intensive care
Authors: R. D Griffiths, C. Jones
Journal: BMJ (1999)

Studies of outcome after intensive care suggest that death rates do not return to normal until 2-4 years after admission. Although some questionnaire studies have reported on morbidity, little published work exists on detailed clinical recovery or longer term residual effects of critical illness. The recovery process may present serious physical, psychological, and social problems for both patients and their families, and these may last for months or years. Although patients who have been in intensive care have often been extremely ill, been at high risk of death, and received care costing tens of thousands of pounds, detailed follow up and targeted support are still rare.



The 5 year mortality rate in intensive care patients is over 3 times that of the general population. However at 2 year survival rates are parallel. Adapted from Niskanen M et al. Crit Care Med 1996;24:1962-7.



Patients on mechanical ventilation are usually discharged from the intensive care unit to the ward when they can breathe unaided. However, several physical problems may still remain. Although these may not be serious enough to keep the patient in intensive care, if left untreated they could lead to readmission. Intensive care staff should therefore follow patients' progress on the ward for a few days to monitor recovery of multisystem disease and assure good continuity of care.

#### Examples of physical disorders after intensive care

The commonest physical problem reported by intensive care patients is severe weakness and fatigue. Patients in intensive care …
Variation of the group 5 grass pollen allergen content of airborne pollen in relation to geographic location and time in season
Authors: Jeroen Buters, Marje Prank, Mikhail Sofiev, Gudrun Pusch, Roberto Albertini, Isabella Annesi-Maesano, Celia Antunes, Heidrun Behrendt, Uwe Berger, Rui Brandao, Sevcan Celenk, Carmen Galan, Łukasz Grewling, Bogdan Jackowiak, Roy Kennedy, Auli Rantio-Lehtimäki, Gerald Reese, Ingrida Sauliene, Matt Smith, Michel Thibaudon, Bernhard Weber, Lorenzo Cecchi
Journal: Journal of Allergy and Clinical Immunology (2015)
Occupational Therapy and Outcomes for Older Adults—Reply
Authors: Florence A. Clark
Journal: JAMA (1998)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
980. Specific Immune Response Against the Plasmodium falciparum CS Protein Mediated by Baculovirus Vectors
Authors: Robert Strauss, Andreas Hüser, Nelson Di Paolo, Andre Liebero, Christian Hofmann
Journal: Molecular Therapy (2005)

Top of pageAbstract
Malaria is the most common tropical disease worldwide. More than 300 million people are infected and about 2-3 million die annually. The most pathogenic form, Malaria tropica, is caused by Plasmodium falciparum. This enormous world-health problem is compounded by parasites becoming resistant to drugs which are commonly used for treatment and also the complete lack of an effective vaccine. Recombinant baculovirus vectors derived from the Autographa californica nuclear polyhedrosis virus (AcNPV) are commonly used as a tool for high-level expression of recombinant proteins of interest in insect cells. Baculovirus vectors can mediate an immune response against an antigen when it is displayed on the viral surface, or when it is expressed from the baculovirus backbone using promoters which are active in mammalian cells. Their ability to accommodate very large inserts of foreign DNA, the lack of a preexisting/neutralizing immunity to baculoviruses in humans and the easy generation of high titer virus stocks are further clear advantages of this vector system for the delivery of a vaccine.

In order to use baculoviruses for an induction of a specific immune response we inserted a P. falciparum circumsporozoite (CS) protein expression cassette, driven by the strong CMV promoter, into the baculoviral genome (AcNPV-CSFVI). An expression of the CS protein in antigen presenting cells (APC) should activate CD8+ T cells via MHC I presentation. The display of a second, recombinant CS peptide in the viral envelope was achieved by fusion of the CS gene to the main baculovirus surface protein gp64 (AcNPV-CS/gp64). This envelope-modified baculovirus mimics the parasite to induce CD4+ T cells and CS specific antibodies following uptake into APC and antigen presentation by MHC II. A third vector (AcNPV-CSFVI-CS/gp64) was constructed to combine expression and presentation of CS antigens in mammalian cells.

Expression of the CS protein upon infection of a mouse muscle cell line with AcNPV-CSFVI or AcNPV-CSFVI-CS/gp64 was confirmed by Western blotting with CS specific antibodies. For vaccination studies, BALB/c mice were injected intramuscularly with 5|[times]|108 pfu of AcNPV-CSFVI, AcNPV-CS/gp64, AcNPV-CSFVI-CS/gp64 or 10 ug recombinant CS protein and boosted after 3 weeks with the same dose. Data of IgG1/IgG2a serum ELISAs showed high CS specific antibody titers for mice injected with AcNPV-CS/gp64. Whereas infection with AcNPV-CSFVI could induce only low levels of antibodies, the combined expression and presentation of CS antigens (AcNPV-CSFVI-CS/gp64) could elicit an even higher IgG2a response. Frequencies of CS specific CD4+ and CD8+ T cells will be analyzed by ELIspot and data will be presented.

Our current efforts are aimed towards targeting baculoviruses infection to dendritic cells by incorporation of specific receptor ligands into the viral envelope.
Wherever Puff Goes
Authors: Walt McDonald
Journal: JAMA (2003)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal