tbuckley/cabot-search huggingface dataset:
222. HSV Amplicon Expression of Single Chain Antibodies Directed Against α-Synuclein Conformers
Authors: Kathleen A. Maguire-Zeiss, Eric Yehling, Rita Giuliano, Mark Sullivan, Howard J. Federoff
Journal: Molecular Therapy (2004)

Parkinson's disease (PD) is a progressive neurodegenerative disorder which targets the nigrostriatal system and is triggered by disparate mechanisms. The hallmark pathobiologic feature of PD is the formation of intracytoplasmic inclusions known as Lewy bodies. Lewy bodies are composed of aggregated proteins, most notably α-synuclein. The selective insult to the dopamine (DA) neuron is posited to involve oxidative injury mediated in part through participation of DA adducts made onto α-synuclein (α-syn). Oxidative stress and DA-derived orthoquinone adducts form onto α-syn promoting the formation of misfolded protein. We hypothesize that therapies specifically designed to interact and reduce toxic α-syn conformers will attenuate disease progression. We are pursuing a gene therapy approach whereby conformation-specific humanized single-chain Fvs (scFvs) are expressed and intended to interfere with misfolded α-syn. Utilizing various conformers of purified α-syn to screen a combinatorial phage display library expressing human immunoglobulin heavy and light chain variable regions we have identified conformation-specific α-syn-specific scFvs. These scFvs have been efficiently expressed in mammalian cells following transduction with an HSV amplicon vector (HSVscFvs). The effect of transduction and expression of intracellular scFvs, termed intrabodies, within stable cell lines overexpressing α-syn is under investigation. HSVscFvs directed against α-syn afford us the opportunity to further investigate the role of α-syn aggregation in the pathogenesis of PD.
QTL influencing autoimmune diabetes and encephalomyelitis map to a 0.15-cM region containing Il2
Authors: Jeffrey A. Encinas, Linda S. Wicker, Laurence B. Peterson, Akiko Mukasa, Cory Teuscher, Raymond Sobel, Howard L. Weiner, Christine E. Seidman, J.G. Seidman, Vijay K. Kuchroo
Journal: Nature Genetics (1999)
RNA Targeting Strategy Regenerates Heart Tissue in Adult Mice
Authors: Tracy Hampton
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Angels in America
Authors: J. H. Tanne
Journal: BMJ (2003)

An epic tale of the early years of AIDS is making many Americans realise how far they have come in the fight for healing and tolerance, and how far they still have to go Rating: ![Graphic][1]</img>![Graphic][2]</img>![Graphic][3]</img>inline-graphic xlink:href=“star”/> You won't see many masterpieces in your lifetime. Angels in America is one. This six hour film, broadcast in two three-hour parts on the premium cable channel HBO in the United States on 7 and 14 December and due to be shown in the United Kingdom next year, tells the story of AIDS in the Reagan era. Adapted from two award winning stage plays, it is an arresting portrayal of the fight against AIDS and for tolerance, shown through the loves and losses of all-too-human characters, in a climate of national hysteria. But it is also far more, setting the story of the early years of AIDS in the much larger context of American culture itself. Angels in America begins in 1985 on the West Side of New York with the funeral of an elderly Jewish woman. As a young girl she bravely fled pogroms, travelling by steerage for a chance at a better life in the United States. Among the prosperous mourners is her grandson, Louis, an … [1]: /embed/inline-graphic-1.gif [2]: /embed/inline-graphic-2.gif [3]: /embed/inline-graphic-3.gif
Granulomatous slack skin: successful treatment withrecombinant interferon-gamma
Authors: T. Kono, T.S. Nagayasu, T. Nakanishi, D. Tsuruta, M. Ishii, S. Taniguchi, T. Takeda, M. Hino
Journal: British Journal of Dermatology (2000)

Journal Article Granulomatous slack skin: successful treatment withrecombinant interferon‐gamma Get access T. Kono, T. Kono Department of Dermatology, Search for other works by this author on: Oxford Academic Google Scholar T.S. Nagayasu, T.S. Nagayasu Department of Dermatology, Search for other works by this author on: Oxford Academic Google Scholar T. Nakanishi, T. Nakanishi Department of Dermatology, Search for other works by this author on: Oxford Academic Google Scholar D. Tsuruta, D. Tsuruta Department of Dermatology, Search for other works by this author on: Oxford Academic Google Scholar M. Ishii, M. Ishii Department of Dermatology, Search for other works by this author on: Oxford Academic Google Scholar S. Taniguchi, S. Taniguchi Department of Dermatology, Osaka General Hospital of the West Japan Railway Company, 1‐3‐5 Tennoji‐cho‐Minami, Abeno‐ku, Osaka 545‐0002, Japan Search for other works by this author on: Oxford Academic Google Scholar T. Takeda, T. Takeda Third Department of Internal Medicine and Search for other works by this author on: Oxford Academic Google Scholar M. Hino M. Hino Department of Clinical Hematology, Osaka City University Medical School, 1‐4‐3 Asahimachi, Abeno‐ku, Osaka 545–8585, Japan Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 142, Issue 2, 1 February 2000, Pages 353–357, https://doi.org/10.1046/j.1365-2133.2000.03308.x Published: 01 February 2000
Abstract 19599: Association of Alpha-Blocker Use with Heart Failure Hospitalization
Authors: Cynthia A Jackevicius, Lingyun Lu, Jeesun Cho, Tina Lee, Zunera Ghaznavi, Alberta Warner
Journal: Circulation (2014)

Introduction: In ALLHAT, the use of alpha-blockers in hypertensive patients without preexisting heart failure (HF) was found to increase the risk of developing HF. Patients with existing HF are often prescribed alpha-blockers for benign prostatic hypertrophy or resistant hypertension. However, it is uncertain whether use of alpha-blockers can worsen existing HF. In this study, we assessed the impact of alpha-blockers on HF hospitalization/emergency room (ER) visits and all-cause hospitalization. Methods: We conducted a retrospective cohort study, using chart abstraction from electronic medical records within a VA Healthcare System, of patients discharged alive after a hospital admission for a primary diagnosis of HF (ICD 9-CM-428.xx) between January 1, 2000 and December 31, 2012. Patients were grouped according to whether they were dispensed a prescription for an alpha-blocker on their HF discharge date. Multivariable logistic regression models were constructed to compare those receiving and those not rec...
Abstract 3316: Revascularization for Chronic Critical Lower Limb Ischemia in Octogenarians
Authors: Florian Dick, Philippe Brosi, Dai-Do Do, Juerg Schmidli, Iris Baumgartner, Nicolas Diehm
Journal: Circulation (2007)

Objective: Advanced age is considered a relative contraindication for surgical revascularization in patients with peripheral arterial occlusive disease (PAD). Aim was to analyze usefulness of endovascular and surgical revascularization for chronic critical limb ischemia (CLI) in patients older than 80 years. Materials: Prospective cohort study of a consecutive series (01/1999 to 06/2004) of patients presenting with CLI. Revascularization cohorts were either surgical or endovascular with conservatively treated patients used as reference. Prospective follow-up after 30 days, 2, 6, and 12 months. Methods: Primary endpoint was sustained clinical success defined as categorical upward shift in clinical symptoms according to Rutherford without major amputation or need for repeated target extremity revascularization (TER). Secondary clinical success was defined accordingly including repeated TER. Mortality, major amputation and TER were secondary endpoints. Results were stratified for age (non-octogenarians [&lt;80 years] vs octogenarians [≥80 years]). Cumulative outcome was determined by Kaplan-Meier method and differences assessed by log-rank tests. Multivariate analysis was performed by Cox proportional regression. Results: 376 patients (158 women, mean age 75.8 ± 10.7 years) with 416 critically ischemic limbs were analysed. Overall, 150 patients were octogenarians (39.9%). 85 limbs were treated surgically (31% octogenarians), and 207 limbs by endovascular means (46% octogenarians). Both sustained and secondary clinical success rates as well as limb salvage were higher in revascularization cohorts as compared to conservatively treated patients regardless of age category ( P =.013, P =.006, and P =.041, respectively). Mortality was significantly higher in octogenarians ( P =.006), and particularly high within 30-days after surgical revascularization (HR 5.35, 95%CI 1.15–24.9). Patient age category did not affect major amputation and TER rate in multivariate analysis. Conclusions: Individually tailored revascularization improves outcome of CLI in octogenarians comparably to non-octogenarians, even so endovascular revascularization should be preferred in octogenarians due to high 30-day mortality associated with surgery.
Transfer of the chemokine receptor CCR5 between cells by membrane-derived microparticles: A mechanism for cellular human immunodeficiency virus 1 infection
Authors: Matthias Mack, Andrea Kleinschmidt, Hilke Brühl, Christiane Klier, Peter J. Nelson, Josef Cihak, Jiurí Plachý, Manfred Stangassinger, Volker Erfle, Detlef Schlöndorff
Journal: Nature Medicine (2000)
Ehlers-Danlos syndromes
Authors: Neeti Ghali, Glenda Sobey, Nigel Burrows
Journal: BMJ (2019)

### What you need to know

Awareness of the Ehlers-Danlos syndromes (EDS) in the UK has risen over the past decade, in part due to high profile of EDS patient organisations and the EDS National Diagnostic Service commissioned in 2009. Significant advances in genetic testing have culminated in the publication of a new international classification in 2017 with 13 types identified.1 Clinicians will see an increasing number of patients, some of whom will suspect a diagnosis of EDS themselves. 

This article is intended to support non-specialist clinicians to consider the diagnosis of this group of conditions in appropriate patients. It discusses the features of the different types of EDS and explains how clinicians can expect their patients to be managed in secondary care.

### How this article was created

The authors are members of the International Consortium on Ehlers-Danlos Syndromes and Related Disorders and participated in literature searches used for the basis of the articles written on the new classification of EDS published in 2017. The authors have more than 10 years’ experience of EDS patient consultations and data collection in the highly specialised EDS service.

Ehlers-Danlos syndromes (EDS) refer to a group of inherited connective tissue …
Mitral Paravalvular Leak
Authors: Daniel B. Spoon, Joseph F. Malouf, Jocelyn N. Spoon, Vuyisile T. Nkomo, Paul Sorajja, Sunil V. Mankad, Ryan J. Lennon, Allison K. Cabalka, Charanjit S. Rihal
Journal: JACC. Cardiovascular imaging (2013)
Coronary vasoregulation in patients with various risk factors in response to cold pressor testing
Authors: Thomas H Schindler, Egbert U Nitzsche, Thomas Munzel, Manfred Olschewski, Ingo Brink, Michael Jeserich, Michael Mix, Peter T Buser, Matthias Pfisterer, Ulrich Solzbach, Hanjörg Just
Journal: Journal of the American College of Cardiology (2003)
Left ventricular mechanical function: clinical correlates, heritability, and association with parental heart failure
Authors: Susan Cheng, Elizabeth L. McCabe, Martin G. Larson, Ming‐Huei Chen, Ewa Osypiuk, Birgitta T. Lehman, Plamen Stantchev, Jayashri Aragam, Scott D. Solomon, Emelia J. Benjamin, Ramachandran S. Vasan
Journal: European Journal of Heart Failure (2014)
NUTRITION IN THE UNITED STATES: A PROGRAM FOR THE PRESENT EMERGENCY AND THE FUTURE
Authors: Russell M. Wilder
Journal: Annals of Internal Medicine (1941)

Article1 June 1941NUTRITION IN THE UNITED STATES: A PROGRAM FOR THE PRESENT EMERGENCY AND THE FUTURERUSSELL M. WILDER, M.D., F.A.C.P.RUSSELL M. WILDER, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-14-12-2189 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThat the present emergency calls for a great increase in the size of our Army and Navy with provision of all that is necessary to make these armed forces into a superlative instrument of war, scarcely anyone denies. That it calls for rapid acceleration of those of our industries which can engage in the manufacture of materials of war, everyone will grant. That to accomplish what needs to be done will entail sacrifice on the part of almost everyone, and longer and more arduous work for many, will be acknowledged. These are self-evident truths. On them, however, is based my... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Rochester, Minnesota*Received for publication April 26, 1941.Read at the meeting of the American Association of Scientific Workers, Chicago, Illinois, April 15, 1941.Presented also at the meeting of the American College of Physicians, Symposium on Nutritional and Metabolic Diseases, Boston, Massachusetts, April 23, 1941. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byABSTRACTS 1 June 1941Volume 14, Issue 12Page: 2189-2198KeywordsArmed forcesForecastingMetabolic disordersNutrition Issue Published: 1 June 1941 PDF downloadLoading ...
Measuring the Long-term Impact of War-Zone Military Service Across Generations and Changing Posttraumatic Stress Disorder Definitions
Authors: Charles W. Hoge
Journal: JAMA Psychiatry (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Psychiatry HomeNew OnlineCurrent IssueFor Authors Podcast Journals JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2024 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Psychiatry journal
Amiodarone, Anticoagulation, and Clinical Events in Patients With Atrial Fibrillation
Authors: Greg Flaker, Renato D. Lopes, Elaine Hylek, Daniel M. Wojdyla, Laine Thomas, Sana M. Al-Khatib, Renee M. Sullivan, Stefan H. Hohnloser, David Garcia, Michael Hanna, John Amerena, Veli-Pekka Harjola, Paul Dorian, Alvaro Avezum, Matyas Keltai, Lars Wallentin, Christopher B. Granger
Journal: Journal of the American College of Cardiology (2014)
Abstract 4341: Metabolomic analysis of HER2-positive breast cancer cells
Authors: Malika K. Sahni, Resmi K. Ravindran, Imran H. Khan, Jason A. Bush, V. V. Krishnan
Journal: Cancer Research (2014)

Abstract Purpose: HER2-positive breast cancer accounts for more than 20% of the diagnosed cases and is characterized by aggressive growth, increased disease recurrence, and poor prognosis. While much of the signaling mechanism(s) have been elucidated, the impact of HER2 positivity on cellular metabolism is not well understood. We examined (1) gene expression profiles of mammalian breast cancer cell lines with increasing HER2 expression levels (MCF10A, MDA-MB-468, MDA-MB-453, HCC1954, HCC2218) and correlated changes to metabolic expression profiles and (2) the effect of serum starvation and pervanadate treatment on the metabolic profiles of these HER2-expressing cell lines. Methods: Our study used a combination of 1H-NMR and 2D-NMR (TOCSY), bioinformatics analyses, and protein expression validation in human breast cancer cell lines (untreated, serum-starved, serum-starved with pervanadate treatment). Results: We found that both gene and metabolic expression profiles of HER2-positive cell lines correlated with leucine and isoleucine biosynthesis (p-value &amp;lt; 0.05). Comparative analyses of profiles from untreated, serum-starved, and serum-starved with pervanadate-treated cell samples revealed that lactate concentration (p-value &amp;lt;0.05) dropped significantly with serum starvation and pervanadate treatment consistent with reduced glycolytic metabolism and potentially mediated through suppression of tyrosine phosphatase activity. Conclusion: This study highlights important new metabolites that are potential biomarkers of HER2-positive breast cancer and suggests alternate/novel pathways to consider for future understanding and treatment. Citation Format: Malika K. Sahni, Resmi K. Ravindran, Imran H. Khan, Jason A. Bush, V. V. Krishnan. Metabolomic analysis of HER2-positive breast cancer cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4341. doi:10.1158/1538-7445.AM2014-4341
Abstract 4174: An integrative genomic analysis for clinically relevant cancer genetic aberration and targeted therapeutic prediction
Authors: HoJoon Lee, Hanlee P. Ji
Journal: Cancer Research (2014)

Abstract While the genomic and genetic basis of many cancers has been partly elucidated, less is known about the identity of specific cancer genes that are associated with clinical parameters such as clinical stage, metastasis, and survival. To address this question, we compiled multiple genomic data types (mutations, copy number alterations, gene expression and methylation status) as well as clinical meta-data for twelve different cancers in the Cancer Genome Atlas Project. We used an elastic-net regularized regression method on the combined genomic data to identify genetic aberrations and their associated cancer genes that are indicators of clinical parameters in each cancer type. To facilitate the identification of clinically actionable genetic aberrations from this cancer genomic data, we utilized genomic and drug response data from the Cancer Cell Line Encyclopedia (CCLE). These expansive data sets of hundreds of cancer cell lines include three genomic data types (mutations, copy number alterations, gene expression) and drug response data. To demonstrate the performance and validity of this approach, we demonstrated that our bioinformatics pipeline successfully identified i) synthetic genes that have their CNVs perfectly associated with stages from a simulated data ii) mutations of TGFBR2, methylation of MLH1 and other driver genes that have genetic changes associated with colorectal cancer (CRC) that demonstrate microsatellite instability CRC from TCGA data, and iii) IDH1 that obtained mutations associated with survival according to glioblastoma (GBM) data. We identify the top ranked genes that delineate key clinical features such as survival analysis or clinical stages for 13 cancers. A fit of the elastic-net regularized regression to more than 200 samples/cancer type and integrative analysis of integrative genomic platforms identified the set of top gene predictors of clinical parameters (TCGA data) and drug response (CCLE data). There are several genes ranked top in multiple cancer types while most of them are unique to a cancer type. These results provide us with new insights into the underlying genetic similarities and differences among 13 cancers; namely, common genetic characteristics that have implications for clinical phenotype. Finally, we are identifying the core set of gene aberrations that are both clinically relevant and drug responsible. Citation Format: HoJoon Lee, Hanlee P. Ji. An integrative genomic analysis for clinically relevant cancer genetic aberration and targeted therapeutic prediction. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4174. doi:10.1158/1538-7445.AM2014-4174
Lipocalin-2 negatively modulates the epithelial-to-mesenchymal transition in hepatocellular carcinoma through the epidermal growth factor (TGF-beta1)/Lcn2/Twist1 pathway
Authors: Yun-Peng Wang, Goung-Ran Yu, Mi-Jin Lee, Sang-Yeop Lee, In-Sun Chu, Sun-Hee Leem, Dae-Ghon Kim
Journal: Hepatology (2013)

Lipocalin-2 (Lcn2) is preferentially expressed in hepatocellular carcinoma (HCC). However, the functional role of Lcn2 in HCC progression is still poorly understood, particularly with respect to its involvement in invasion and metastasis. The purpose of this study was to investigate whether Lcn2 is associated with the epithelial-mesenchymal transition (EMT) in HCC and to elucidate the underlying signaling pathway(s). Lcn2 was preferentially expressed in well-differentiated HCC versus liver cirrhosis tissues, and its expression was positively correlated with the stage of HCC. The characteristics of EMT were reversed by adenoviral transduction of Lcn2 into SH-J1 cells, including the down-regulation of N-cadherin, vimentin, alpha-smooth muscle actin, and fibronectin, and the concomitant up-regulation of CK8, CK18, and desmoplakin I/II. Knockdown of Lcn2 by short hairpin RNA (shRNA) in HKK-2 cells expressing high levels of Lcn2 was associated with EMT. Epidermal growth factor (EGF) or transforming growth factor beta1 (TGF-β1) treatment resulted in down-regulation of Lcn2, accompanied by an increase in Twist1 expression and EMT in HCC cells. Stable Lcn2 expression in SH-J1 cells reduced Twist1 expression, inhibited cell proliferation and invasion in vitro, and suppressed tumor growth and metastasis in a mouse model. Furthermore, EGF or TGF-β1 treatment barely changed EMT marker expression in SH-J1 cells ectopically expressing Lcn2. Ectopic expression of Twist1 induced EMT marker expression even in cells expressing Lcn2, indicating that Lcn2 functions downstream of growth factors and upstream of Twist1.Together, our findings indicate that Lcn2 can negatively modulate the EMT in HCC cells through an EGF (or TGF-β1)/Lcn2/Twist1 pathway. Thus, Lcn2 may be a candidate metastasis suppressor and a potential therapeutic target in HCC.