tbuckley/cabot-search huggingface dataset:
Abstract P441: Walking Patterns on Weekdays, Weekends, and Holidays in a Large, Metropolitan U.S. Airport: The Walk to Fly Study
Authors: Janet E Fulton, Prabasaj Paul, Virginia M Frederick, Joan M Dorn
Journal: Circulation (2014)

Objective: Many airports provide environments conducive for walking but little is known about walking patterns. The objective of this study is to characterize airport walking volume and mode choice by day of the week and around holidays. Methods: Individuals entering the transportation mall of a large, metropolitan U.S. airport may ride the train, walk, or use a moving walkway to get to their gate. Six ceiling-mounted, infrared sensors count individuals who enter the transportation mall, and those who walk or use the moving walkway. Train riders are the difference between the entry, and the walk and walkway counts. Holiday travel days occurred before and after the 2013 July 4 (July 3 and 7) and Labor Day (Aug 30 and Sept 3) holidays. Descriptive and logistic regression analyses were performed using R version 2.14.1. Results: Data collection occurred from June 15 -- Oct 2, 2013. On average, more than 46,000 individuals entered the transportation mall daily where 89.9% rode the train, 4.6% walked, and 5.5% used the moving walkway. The mean volume of individuals ranged from 34,430 on Saturdays to 48,411 on Mondays (Figure). The mean percentage of individuals walking or using the moving walkway was 10.5% on weekdays and 9.3% on weekends. The odds of walking or using the moving walkway were lower on weekends (versus weekdays) [OR 0.873 (95% CI: 0.867-0.879)] and around holidays (versus non-holidays) [OR 0.912 (0.898-0.925)]. Conclusion: In a large, metropolitan U.S. airport, walking or using the moving walkway is less common on weekends and on days surrounding holidays. Airport walking interventions may need to consider differences in walking patterns on weekdays, weekends, and holidays.
Phthalanilide Inhibition of Protein Synthesis in a Cell-free L1210 Mouse Ascites Leukemia System
Authors: Manuel Ochoa, Alfred Gellhorn, William B. Benjamin
Journal: Cancer Research (1964)

The incorporation of amino acids into polypeptides was inhibited in cell-free extracts of L1210 cells from mice pretreated with 2-chloro-4′-4″-di-2-imidazolin-2-yl terephthalanilide. The impairment of the protein synthetic reactions was found both in the presence of endogenous informational RNA and following the addition of polyuridylic and polyadenylic acids. The site of inhibition of the protein synthetic capacity was localized to the microsomes.
Abstract P130: Association between Weight Loss Program Attendance and Weight Loss among Adults with Serious Mental Illness
Authors: Gerald J Jerome, Deborah R Young, Arlene T Dalcin, Joseph V Gennusa, Meghan Oefinger, Airong Yu, Nae-Yuh Wang, Lawrence J Appel, Gail L Daumit
Journal: Circulation (2014)

Introduction: Obesity is epidemic among adults with serious mental illness (SMI) (e.g., schizophrenia, bipolar disorder) and evidenced-based weight loss programs are needed for this group. We examined the association between attendance to program sessions and weight change among adults with SMI who were enrolled in a lifestyle-based weight loss trial. Methods: Overweight and obese adults with SMI were randomized to either a control group (n=147) or an active intervention group (n=144) that received weight loss classes (3/month), individual weight management sessions (monthly), and group exercise classes (3/week) during the first 6 months. These classes tapered off during months 7-18. Study staff maintained attendance records. Standardized weight was assessed at baseline, 6 and 18 months. Regression analyses included weight change as the dependent variable and controlled for sex, age, and site. Results: The active intervention group was 51% female with an average age of 47 years. Main results have reported significant weight loss at 6 (M=-1.7kg, SD=4.6) and 18 months (M=-3.4kg, SD=7.4). Attendance at 6 and 18 months are reported in the figure below. Attendance was not associated with weight change at 6 months. Overall percent attendance was not associated with 18 month weight change (B=-0.02, p=-.59). A second regression model included percent attendance individually for each of type of session and group weight loss attendance (B=-0.10, p=.04) was associated with weight loss; however, individual weight loss sessions (B=0.002, p=.95), and group exercise (B=0.08, p=.08) were not associated with weight loss. Conclusion: Although overall attendance was not associated with weight loss, attendance to the group weight loss session was associated with weight loss at 18 months. It is not clear if the content of the weight loss groups was particularly effective or if adherence to these sessions was the strongest indicator of adherence to the behavioral recommendations.
1054. Development of Adeno-Associated Virus (AAV) Vectors for Gene Therapy in the Mouse Model for Glycogen Storage Disease Type Ia (GSD-Ia)
Authors: Dwight D. Koeberl, Baodong Sun, Luis Franco, Andrew Bird, Eric Faulkner, Y.-T. Chen
Journal: Molecular Therapy (2004)

The deficiency of glucose-6-phosphatase (G6Pase) underlies glycogen storage disease type Ia (GSD-Ia, also known as von Gierke disease; MIM 232200), an autosomal recessive disorder of metabolism associated with life-threatening hypoglycemia, growth retardation, and severe long-term complications. Regulation of G6Pase at the level of transcription occurs in response to glucose and insulin levels. We investigated the hypothesis that regulation of G6Pase expression is critical to the success of gene therapy in GSD-Ia. Indeed, sustained G6Pase expression in GSD-Ia could further disrupt glucose metabolism by causing hyperglycemia and depleting liver glycogen.
A Study of Mouse Ovarian Function after Irradiation
Authors: Charles A. Ely
Journal: Cancer Research (1960)

Intermittent injection of antigonadotropic sera into irradiated mice resulted in regularly renewed vaginal cycles. The frequency of newly initiated cycles fell the 3d week after exposure but returned to previous levels within a few weeks. Antigonadotropic serum prevented this fluctuation. The mechanism of induction of ovarian tumors is discussed in the light of these findings.
Abstract 14049: High-Molecular-Weight Polyethylene Glycol (PEG) Inhibits Myocardial Ischemia-Reperfusion Injury and Improves Ventricular Function and Survival
Authors: Xianyao Xu, Jennifer L Philip, Abdur Razzaque, James W Lloyd, Charlie M Muller, Shahab A Akhter
Journal: Circulation (2014)

Cardiac ischemia-reperfusion (I-R) injury remains a significant clinical problem as there are no therapies available to minimize the cell death that can lead to impaired function and heart failure. We have shown that high-molecular-weight PEG (15-20kD) can protect cardiac myocytes in vitro from hypoxia-reoxygenation injury. In this study, we investigated the potential protective effects of PEG in vivo. Adult male rats underwent left thoracotomy and LAD occlusion for 60 minutes followed by either 48 hours (hrs) or 4 weeks (wks) of reperfusion. 1 mL of 10% PEG solution or saline (PBS) control (n=10 per group) was administered intravenously (IV) immediately prior to reperfusion. Fluorescein-labeled PEG was robustly visualized in the myocardium 1 hr after IV delivery. Survival at 4 wks post-I-R was greater in the PEG group: 90% vs. 60% in PBS controls. The PEG group had full recovery of LV ejection fraction at 4 wks vs. 20% decline from baseline in the PBS group (p
Abstract 1508: A highly sensitive and reliable methodology (ARMS-PM system) for detection of K-ras mutations in FFPE samples
Authors: Wei-Tao Duan, Xiujuan Wang, Lihong Hu, Feng Zhu, Dehua Yu
Journal: Cancer Research (2014)

Abstract The association between the activating mutations of K-ras gene and poor clinical response to current targeted therapies against epidermal growth factor receptor (EGFR ) such as cetuximab (Erbitux®) and panitumumab (Vectibix®) in colorectal cancer (CRC) has been well documented. Therefore detection of K-ras mutation status will guide effective therapeutical options for patients with CRC. Here we developed a new method for the rapid and reliable detection of K-ras mutations by using a combination of the modified mutation-specific ARMS primers, peptide nucleic acid (PNA) clamping and taqman-MGB probes, named ARMS-PM system. In the ARMS-PM system, the wild-type K-ras DNA is completely blocked by PNA at a higher annealing temperature, and the mutant gene is selectively amplified by the modified ARMS primers and the amplicon extending signals are collected by the taqman-MGB probe at the same time. The ARMS-PM system can detect approximately 10 copies of the mutant gene stably in somatic cells at the background of 10ng genomic DNA. In addition, the premixed reaction was introduced into this system to allow the assay to be completed in only one step of sample loading, which minimizes the potential contamination significantly. By using this assay, we analyzed the K-ras mutations in 1014 Chinese CRC and 205 Chinese lung cancer FFPE samples in comparison with Sanger sequencing. Our results showed that the positive rates were 38.6% and 10.2% 38.0% in CRC and lung cancer samples respectively, which were higher than that measured by Sanger sequencing (38.0% and 7.1%). The coincidence rates of the two methods were 97.3% for the positive samples and 97.7 for the negative samples. The total coincidence rate was 97.8%. In conclusion, our study provides a basis for the detection of somatic mutations in K-ras in a routine clinical setting. The ARMS-PM assay allows the detection of the activating K-ras mutations in FFPE samples with better sensitivity and feasibility than the current assays used in clinic. &amp;lt;!–EndFragment–&amp;gt; Citation Format: Wei-Tao Duan, Xiujuan Wang, Lihong Hu, Feng Zhu, Dehua Yu. A highly sensitive and reliable methodology (ARMS-PM system) for detection of K-ras mutations in FFPE samples. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1508. doi:10.1158/1538-7445.AM2014-1508
Abstract 4200: Interaction between mPGES-1 and iNOS promotes human melanoma progression
Authors: Sun-Hee Kim, Yuuri Hashimoto, Suhendan Ekmekcioglu, Elizabeth A. Grimm
Journal: Cancer Research (2014)

Abstract Melanoma cells in vivo are well known to express varying inflammatory features, which is not limited by somatic mutational subclass. Among the most prevalent of inflammatory markers are iNOS (inducible Nitric Oxide Synthase) generated NO production and COX-2/PGE2, and for which their active pathways have been shown to enhance carcinogenesis and tumor progression, stimulate angiogenesis, support tumor growth, and promote metastasis. COX-2 inhibitors are well studied agents that inhibit tumor growth and prevent tumor development including melanoma, but the focus of research has shifted to develop inhibitors for enzymes, which are downstream of COX-2, especially microsomal PGE2 synthase-1 (mPGES-1), due to the cardiovascular risk associated with the initial COX-2 inhibitory drugs. Here, we demonstrate crosstalk between iNOS/NO and mPGES-1/PGE2 signaling pathways and suggest that mPGES-1 is a novel potential therapeutic target in human melanoma employing a spectrum of well-defined human cell lines, as well as human tissues. Our melanoma stage III survival Tissue MicroArray analysis shows that the expression of mPGES-1 is elevated in 65% of human stage III melanomas and intense expression of mPGES-1 is significantly associated with the short-term survival of patients. More interestingly, about 90 % of patient samples which express mPGES-1 were also positive for iNOS. In addition, treatment of human melanoma cells with NO donors or constitutively-driven expression of iNOS resulted in increased PGE2 production, which was suppressed by mPGES-1 inhibition, indicating that the pathway is active and targetable. Moreover, iNOS was found to interact directly with mPGES-1, and iNOS or NO donors enhance mPGES-1 activity in melanoma cells and in vitro, suggesting that iNOS-mediated NO promotes PGE2 production through mPGES-1 activation. mPGES-1 inhibition by its shRNA or specific inhibitors suppressed human melanoma cell viability and migration. Collectively, these findings suggest that the interaction between iNOS and mPGES-1 is associated with human melanoma progression and could be considered as a novel target for translational application in melanoma growth regulation. Citation Format: Sun-Hee Kim, Yuuri Hashimoto, Suhendan Ekmekcioglu, Elizabeth A. Grimm. Interaction between mPGES-1 and iNOS promotes human melanoma progression. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4200. doi:10.1158/1538-7445.AM2014-4200
Making Access to Quality and Affordable Health Care a Reality for Every American
Authors: John S McCain
Journal: JAMA (2008)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 20167: The CD38 Inhibitor Luteolinidin Ameliorates Ischemia/reperfusion Injury Through Preservation of NADP(H) and NAD(H)
Authors: James J Boslett, Francesco De Pascali, Craig Hemann, Jay Zweier
Journal: Circulation (2014)

Introduction: In postischemic hearts impaired endothelial function occurs with endothelial nitric oxide synthase (eNOS) dysfunction limiting vasodilation and tissue perfusion. We have observed that during cardiac ischemia/reperfusion (I/R), the eNOS substrate NADPH is depleted with >80% loss in the endothelium and NADPH repletion restores eNOS activity and vasodilation. To determine the mechanism of NADPH depletion, the NAD(P)-consuming enzyme CD38 was investigated. There has been a need for a potent and specific CD38 inhibitor. Recently, the flavonoid luteolinidin has been shown to inhibit CD38 in the low μM range. We hypothesized that luteolindin as a potent CD38 inhibitor would elicit protection of endothelial and myocardial function in the postischemic heart. Methods: Isolated Langendorff perfused rat hearts were treated with luteolinidin (50 μM) (n=8) or vehicle control (n=10) and subjected to 30 min I and 30 min R. Contractile function was measured by intraventricular balloon, and coronary flow by D...
Changes in patient ventilator asynchrony (PVA) with 3 months home mechanical ventilation (HMV) therapy
Authors: Michelle Ramsay, Swapna Mandal, Eui-Sik Suh, Joerg Steier, Anita Simonds, Nicholas Hart
Journal: European Respiratory Journal (2013)

Introduction:PVA adversely affects respiratory muscle unloading and we hypothesised that this contributes to reducing patient adherence to HMV.  Methods:Patients admitted for initiation of HMV were enrolled and titrated using capnography and oximetry.We measured parasternal electromyography as a marker of neural respiratory drive, respiratory plethysmography and airway pressure waveforms to characterise PVA non-invasively. Measurements were collected at baseline and 3 months. PVA was scored by our standard definitions and expressed as percentage of total breaths analysed.  Results:15 patients(8 male) participated; 7 neuromuscular disease(NMD), 5 chronic obstructive pulmonary disease(COPD) and 3 obesity hypoventilation syndrome(OHS). 29,531 breaths were analysed. Overall, PVAs affected 28% of breaths at baseline dropping to 23% breaths at 3 months. Initially OHS patients had higher levels of PVA followed by COPD and NMD patients. In OHS patients PVAs reduced by 19% at 3 months whilst in COPD and NMD PVAs increased by 0.9% and 3.5% respectively. Median daily ventilator adherence at 3 months was 6:42h(1:29-7:40), no significant correlation was found between change in PVAs and adherence to HMV (r=-0.2, p=0.5).  Conclusion:PVA did not change significantly with 3 months HMV therapy. The incidence of PVA remains high at 3 months, albeit with a fall in the OHS group; changes in PVA were not related to ventilator adherence.
Abstract 12814: Incidence of Stroke or Systemic Embolism in Paroxysmal versus Sustained Atrial Fibrillation: From the Fushimi Af Registry
Authors: Kensuke Takabayashi, Yasuhiro Hamatani, Mitsuru Ishii, Hisashi Ogawa, Masahiro Esato, Yeong-Hwa Chun, Hiromichi Wada, Koji Hasegawa, Mitsuru Abe, Masaharu Akao
Journal: Circulation (2014)

Background: Atrial fibrillation (AF) increases the risks of stroke. Previous studies revealed patients with paroxysmal AF (PAF) have a risk of stroke similar to that in patients with sustained (persistent or permanent) AF (SAF). Methods: The Fushimi AF Registry, a community-based prospective survey, was designed to enroll all of the AF patients in Fushimi-ku, Kyoto, Japan. At present, we have enrolled 3,985 patients from March 2011 to April 2014. One-year follow-up was completed in 3,189 patients as of April 2014. We compared the baseline clinical characteristics and one-year outcome between PAF (n=1,534, 48.1%) and SAF (n=1,655, 51.9%). Results: Patients with PAF were younger (PAF vs. SAF: 72.3±11.7 vs. 74.9±9.9 years; p&lt;0.01), less likely to have a history of stroke (15.0% vs. 22.1%; p&lt;0.01), heart failure (17.3% vs. 34.9%; p&lt;0.01), and had lower CHADS2 score (1.82±1.29 vs. 2.22±1.35; p&lt;0.01). During the one-year follow-up period, there was no significant difference in all-cause death (116 (7.6%) vs. 137 (8.3%); p=0.45) or major bleeding (25 (1.6%) vs. 29 (1.8%); p=0.78) between PAF and SAF. In patients with PAF, incidence of stroke or systemic embolism (SE) was less (29 (1.9%) vs. 52 (3.1%); p=0.02) and so was the hospitalization for heart failure (43 (2.8%) vs. 83 (5.0%); p&lt;0.01). In subgroup of patients with CHADS2 score ≥2, there was no significant difference in the incidence of stroke or SE between PAF and SAF (p=0.58) (figure B). In contrast, PAF was associated with lower incidence of stroke or SE in patients with CHADS2 score 0 or 1 (p=0.02) (figure A). After the adjustment by gender and established risk factors (components of CHADS2 score) in multiple logistic regression models, PAF was independently associated with lower incidence of stroke or SE in CHADS2 score 0 or 1 (adjusted odds ratio, 0.22; 95% confidence interval, 0.05 to 0.72; p=0.01). Conclusion: PAF was independently associated with lower incidence of stroke or SE in low risk patients with CHADS2 score 0 or 1.
Abstract 15272: Wnt11 Delivered in Mesenchymal Stem Cell-Derived Media Promotes Angiogenesis Through Non-canonical Wnt-PKC-JNK Pathway
Authors: Jingcai Wang, Bin Yu, Min Gong, Honghai Liu, Yigang Wang, Ronald W. Millard, Meifeng Xu
Journal: Circulation (2014)

Background: Wnt11 is involved in many developmental and cellular processes. We have previously reported that Wnt11 overexpressing (MSC Wnt11 ) significantly improved cardiac function and increased angiogenesis. Here, we aimed to elucidate how Wnt11 delivered by MSC increase blood vessel formation. Methods and Results: An acute myocardial infarction (MI) model in SD rats was developed by ligation of the left anterior descending (LAD) coronary artery. Conditioned medium (CdM) collected from MSC Wnt11 (CdM Wnt11 ) was injected immediately into the ischemic border area. Rats treated with CdM Wnt11 showed a significantly improved cardiac function. Furthermore, CdM Wnt11 increased blood vessel density and regional blood flow in ischemic myocardium. Fluorescent immunostaining showed that both vWF-positive single endothelial cell numbers and microvessel numbers were significantly increased in CdM Wnt11 treated myocardium. In vitro studies showed that CdM Wnt11 significantly increased number and length of capillary-like structure (CLS) formation and promoted human umbilical vein endothelial cells (HUVECs) migration. These results were confirmed by directly treating HUVECs with recombinant Wnt11 proteins (5μg/ml) or over-expressing Wnt11 in HUVECs (H Wnt11 ). However, these effects could be abolished by using a Wnt11 neutralizing antibody. Immunostaining and Western blotting results revealed upregulation of p-pan-PKC and p-JNK in H Wnt11 . Real-time PCR analysis indicated that the expression of novel PKCs including PKCδ, PKCε, PKCη, and PKCθ was significantly upregulated in H Wnt11 . Moreover, the effect of Wnt11 on CLT formation and HUVECs migration were abrogated when the JNK inhibitor SP600125 at 5 μM and the PKC inhibitor Calphostin-C at 0.1 μM were added to the culture system. Conclusion: Our data suggests, for the first time, that Wnt11 in MSC-derived media improved cardiac function and promoted angiogenesis in ischemic myocardium. The effect of Wnt11 in angiogenesis is mediated by novel PKCs, with JNK as its downstream mediator.