tbuckley/cabot-search huggingface dataset:
Abstract 2672: Characterization of Hsp90/Cdc37 interaction and their critical residues using luciferase fragment complementation imaging
Authors: Yiqun Jiang, Denzil Bernard, Yanke Yu, Tao Zhang, Yanyan LI, Xueqi Fu, Shaomeng Wang, Duxin Sun
Journal: Cancer Research (2010)

Abstract The purpose of this study is to investigate Hsp90/Cdc37 interaction and identify residues critical for the interaction using real-time luciferase complementation imaging and computational modeling. Molecular imaging using a split Renilla luciferase fragment complementation (SRL-FC) was established to monitor real-time Hsp90/Cdc37interactions in living cells. Computational modeling and molecular dynamics simulations were used to evaluate the interaction interface of Hsp90/Cdc37 complex and critical residues in the interaction interface were identified for mutagenesis. Western Blotting and co-immunoprecipitation were used to determine protein expression and complex formation. The molecular imaging in living cells showed that Hsp90 and Cdc37 helped the complementation of N-terminus of Renilla luciferase (fused with Hsp90; N-RL-Hsp90) and C-terminus of Renilla luciferase (fused with Cdc37, Cdc37-C-RL) with 170-fold higher luciferase activity than controls without Hsp90/Cdc37. This suggests that the SRL-FC is sensitive and specific to monitor the interaction of full length human Hsp90 and Cdc37. Molecular dynamics simulations revealed that the interface was constituted by a series of residues interacting through hydrophobic or polar interactions. Mutagenesis confirmed that mutations in Hsp90 (A121N, Q133A, F134A) and mutations in Cdc37 (M164K, R167A) led to the loss of complementation of N-RL-Hsp90 and Cdc37-C-RL (5 to 10-fold lower luciferase activity compared to wild-type), indicating these residues are critical in Hsp90/Cdc37 interactions. In comparison, mutations in Hsp90 (E47A, S113A) and mutations in Cdc37 (A204E) only decreased 50% of the complementation of N-RL-Hsp90 and Cdc37-C-RL, which suggests that these residues contribute less to Hsp90/Cdc37 interaction. In contrast, mutations of Hsp90 (R46A, S50A) and mutation in Cdc37 (L165H) did not change the complementation of N-RL-Hsp90 and Cdc37-C-RL, indicating that these residues did not contribute to Hsp90/Cdc37 interaction. The data suggests that molecular imaging using split Renilla luciferase fragment complementation (SRL-FC) can be used to identify the critical residues in Hsp90/Cdc37 interaction. Although Hsp90/Cdc37 interactions are through both hydrophobic and polar interactions, mutation in a single amino acid residue, including Ala121, Gln133, Phe134 in human Hsp90 and Met164, Arg167 in human Cdc37, is sufficient to disrupt Hsp90/Cdc37 interaction. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2672.
A mouse model of human oral-esophageal cancer
Authors: Oliver G. Opitz, Hideki Harada, Yasir Suliman, Ben Rhoades, Norman E. Sharpless, Ralph Kent, Levy Kopelovich, Hiroshi Nakagawa, Anil K. Rustgi
Journal: Journal of Clinical Investigation (2002)

Squamous cancers of the oral cavity and esophagus are common worldwide, but no good genetically based animal model exists. A number of environmental factors as well as genetic alterations have been identified in these cancers, yet the specific combination of genetic events required for cancer progression remains unknown. The Epstein-Barr virus ED-L2 promoter (L2) can be used to target genes in a specific fashion to the oral-esophageal squamous epithelium. To that end, we generated L2–cyclin D1 (L2D1+) mice and crossbred these with p53-deficient mice. Whereas L2D1+ mice exhibit a histologic phenotype of oral-esophageal dysplasia, the combination of cyclin D1 expression and p53 deficiency results in invasive oral-esophageal cancer. The development of the precancerous lesions was significantly reversed by the application of sulindac in the drinking water of the L2D1+/p53+/– mice. Furthermore, cell lines derived from oral epithelia of L2D1+/p53+/– and L2D1+/p53–/– mice, but not control mice, formed tumors in athymic nude mice. These data demonstrate that L2D1+/p53+/– mice provide a well-defined, novel, and faithful model of oral-esophageal cancer, which allows for the testing of novel chemopreventive, diagnostic, and therapeutic approaches.
Diagnosis of Diabetic Eye Disease
Authors: Elliot J. Sussman
Journal: JAMA (1982)

The correct diagnosis of proliferation diabetic retinopathy is essential, because it is a treatable disease and missing the diagnosis can lead to the patient becoming blind. We examined the ability of internists and ophthalmologists to diagnose proliferative retinopathy under optimal conditions. Twenty-three physicians performed retinal examinations on ten diabetic patients and one normal patient with dilated pupils. Physician examiners were members of a university medical center and included 10 internists, 2 diabetologists, 4 senior medical residents, 4 general ophthalmologists, and 3 ophthalmologists who were subspecialists in retinal disease. Correct diagnosis was determined separately by the consensus of three ophthalmologists specializing in retinal disease, who reviewed seven-view stereo fundus photographs and medical charts. Of a possible 483 individual eye a examinations, 438 were completed. The overall error rate was 61%. The error rate for missing the diagnosis of proliferative retinopathy varied from 0% for retinal specialists to 49% for internists, diabetologists, and medical residents. We conclude that potentially serious mistakes in diagnosis are currently made by the physicians who care for most diabetic patients. Experience and specialized knowledge lessen that the error rate. Further education or greater use of referrals may be necessary to provide optimal patient care.
Increase in S-100b protein content in thyroid carcinoma
Authors: Akio Nagasaka, Hayato Umekawa, Hiroyoshi Hidaka, Katsumi Iwase, Akira Nakai, Yutaka Ariyoshi, Takako Ohyama, Toshihiro Aono, Hifumi Nakagawa, Susumu Ohtani, Shigehiro Shinoda, Shuichi Miyakawa, Kyohei Kawase, Kaoru Miura
Journal: Metabolism (1987)
LACK OF IDENTITY IN NEUTRALIZING AND HEMAGGLUTINATION-INHIBITING ANTIBODIES AGAINST INFLUENZA VIRUSES
Authors: Duard L. Walker, Frank L. Horsfall
Journal: The Journal of Experimental Medicine (1950)

There is an exponential linear relationship between the quantity of influenza virus neutralized and the quantity of immune serum employed in in ovo neutralization. The slope of the neutralization line is extremely steep. The concentration of neutralizing antibody can be measured with considerable precision in ovo if the constant virus-varying serum technique is utilized. The amounts of hemagglutination-inhibiting and neutralizing antibodies which are absorbed by a given quantity of influenza virus (PR8) were found to be predictable and the degree of reactivity of these two antibodies was shown to be directly related to the extent of immunization. It was demonstrated that there are marked discrepancies in correlation between antibody titers obtained by in vitro hemagglutination-inhibition and in vivo neutralization techniques and that neutralizing antibody is preferentially absorbed by a given quantity of virus. Inasmuch as the results were found not to be attributable to peculiarities of the techniques employed, it appears that the antibodies measured by hemagglutination-inhibition in vitro and by neutralization in vivo are not identical.
270 Glypican-3 (GPC3) expression in hepatocellular carcinomas and preneoplastic liver lesions
Authors: X.Y. Xiao, S. Dubois, S. Tessiore, E. Degos, J. Belghiti, M. Allegretta, R. Guttmann, P. Bedossa, V. Paradis
Journal: Journal of Hepatology (2006)
Cecal Diverticulitis: An Unusual Endoscopic Finding
Authors: F. J. Fernández-Pérez, C. De Sola, N. Fernández-Moreno, R. Rivera-Irigoin, E. Ubiña-Aznar, G. García-Fernández, J. M. Navarro-Jarabo, P. Moreno-Mejías, A. Ibañez-Pinto, A. Sanchez-Cantos
Journal: Endoscopy (2004)

Figure 1 Colonoscopy must be avoided whenever colonic diverticulitis is suspected. However, some patients are diagnosed endoscopically, most of them with left-sided diverticulitis. A 74 year-old woman presented with lower right abdominal pain. Her physical examination did not show any evidence of an abdominal mass or any peritoneal signs. Complementary studies showed a neutrophil count of10 950/ml and cecal wall thickening, with distortion of the surrounding fatty tissue on abdominal computed tomography, suggesting a colonic tumor.
In vivo assessment of diaphragm contraction by ultrasound in normal subjects.
Authors: J Ueki, P F De Bruin, N B Pride
Journal: Thorax (1995)

BACKGROUND--Ultrasound allows observation of the thickness of the diaphragm in the zone of apposition in vivo during relaxation and maximum inspiratory efforts. METHODS--Changes of diaphragm thickness were studied by B mode (two dimensional) ultrasound in 13 healthy men aged 29-54 years in the seated position. A high resolution 7.5 MHz ultrasound transducer was held perpendicular to the chest wall in the line of a right intercostal space between the anteroaxillary and mid-axillary lines to observe the diaphragm in the zone of apposition 0.5-2 cm below the costophrenic angle. The changes of thickness were observed while breath holding at total lung capacity (TLC), functional residual capacity (FRC), and residual volume (RV). At FRC the thickness while relaxing against a closed mouthpiece and during a maximum inspiratory mouth pressure (PImax) manoeuvre was recorded. The thickening ratio (TR) was calculated as TR = thickness during PImax manoeuvre/thickness while relaxing. RESULTS--Mean (SD) thickness was 4.5 (0.9) mm at TLC, 1.7 (0.2) mm at FRC, and 1.6 (0.2) mm at RV. During the PImax manoeuvre at FRC mean thickness increased from 1.7 (0.2) mm during relaxation to 4.4 (1.4) mm, while mean PImax and TR were -104 (33) cm H2O and 2.6 (0.7), respectively. There was a high degree of correlation between TR and the pressure achieved during the maximum inspiratory manoeuvre (r = -0.82). CONCLUSIONS--Ultrasound provides a non-invasive assessment of diaphragm thickness with change of lung volume and during the PImax manoeuvre which should prove useful in assessing diaphragm mass and contraction in respiratory and muscle disease.
Elevated expression of CUEDC2 protein confers endocrine resistance in breast cancer
Authors: Xin Pan, Tao Zhou, Yan-Hong Tai, Chenguang Wang, Jie Zhao, Yuan Cao, Yuan Chen, Pei-Jing Zhang, Ming Yu, Cheng Zhen, Rui Mu, Zhao-Fang Bai, Hui-Yan Li, Ai-Ling Li, Bing Liang, Zhao Jian, Wei-Na Zhang, Jiang-Hong Man, Yan-Fei Gao, Wei-Li Gong, Li-Xin Wei, Xue-Min Zhang
Journal: Nature Medicine (2011)
Cognitive-Behavioral Therapy for Threshold and Subthreshold Anorexia Nervosa: A Three-Year Follow-Up Study
Authors: Valdo Ricca, Giovanni Castellini, Carolina Lo Sauro, Edoardo Mannucci, Claudia Ravaldi, Francesco Rotella, Carlo Faravelli
Journal: Psychotherapy and Psychosomatics (2010)

&lt;i&gt;Background:&lt;/i&gt; Few long-term follow-up studies have evaluated the response to psychotherapeutical interventions in anorexia nervosa (AN). The effectiveness of individual cognitive-behavioral therapy (CBT) and the possible predictors of outcome in outpatients suffering from threshold and subthreshold AN (s-AN) were evaluated. &lt;i&gt;Methods:&lt;/i&gt; At the beginning (T0) and at the end of treatment (T1), and 3 years after the end of treatment (T2), 53 subjects with AN and 50 with s-AN (all DSM-IV criteria except amenorrhea or underweight) were assessed by a face-to-face clinical interview and by self-reported questionnaires for eating attitudes and behavior (Eating Disorder Examination Questionnaire), body uneasiness (Body Uneasiness Test) and general psychopathology (Symptom Checklist, Beck Depression Inventory, State-Trait Anxiety Inventory). &lt;i&gt;Results:&lt;/i&gt; No deaths occurred during the treatment and the follow-up period. At the end of the follow-up 34 subjects (33%) initially enrolled in the study obtained a full recovery. AN and s-AN patients did not show significant differences on most of the clinical measures at baseline and in terms of treatment response (T1, T2). The reduction in weight and shape concerns was associated with weight gain at T1 and T2, and the shape concern level at baseline represented the main risk factor for recovery and treatment resistance. According to survival analysis, patients with high shape concern had a lower probability of remission across time. &lt;i&gt;Conclusions:&lt;/i&gt; The distinction between threshold and subthreshold AN does not seem to be of clinical relevance in terms of response to CBT. Shape concern rather than demographic or general psychopathological features represents the best predictor of outcome for CBT.
Screening for Colorectal Cancer
Authors: Jane F. Desforges, David F. Ransohoff, Christopher A. Lang
Journal: New England Journal of Medicine (1991)

GOLORECTAL cancer is a leading cause of death from cancer in the United States, with approximately 140,000 new cases and 60,000 deaths per year.1 A 50-year-old person has about a 5 percent risk of having colorectal cancer by the age of 80 and a 2.5 percent risk of dying from it.2 To reduce the morbidity and mortality due to colorectal cancer, some professional organizations have proposed that asymptomatic persons without known risk factors be screened to detect cancer at a curable stage.3 4 5 The rationale for screening is based on the widely held view6 that most colorectal cancers are the product . . .
ABC of learning and teaching: Web based learning
Authors: J. McKimm
Journal: BMJ (2003)

Many of us use the internet or the “web” (world wide web) as a source of information. In medical education, the web is increasingly used both as a learning tool to support formal programmes and as a means of delivering online learning programmes. What can educators do to ensure that the potential of the web is used effectively to support both their own learning and that of their students?

Much of the literature on web based learning shows that one of the main barriers to the effective use of teaching materials is the technology (for example, poor access, slow downloading) rather than the design of the learning materials themselves. Some of these issues are discussed later in the article, but it is vital that teachers take on expert help with technical issues in the planning, design, and delivery of web based learning programmes. Through programming and the use of “plug-ins” (programs that can be downloaded from the internet), designers can produce interactive course materials containing online activities (such as self assessments), animations, and simulations. These can improve learning and are often more enjoyable and meaningful for learners.

#### Glossary

E-conferencing —Use of online presentations and discussion forums (in real time or stored as downloadable files on a website) to avoid the need for participants to travel

E-learning —Learning through electronic means, such as via the web (see world wide web), an intranet, or other multimedia materials

HTML (hypertext markup language) —The language used to create web pages. HTML files can also contain links to other types of files including wordprocessed files, spreadsheets, presentation slides, and other web pages

Hyperlinks —Links in web pages that enable the user to access another web page (either on the same or a different site) with just one mouse click

Internet— A global network of computers divided …
580 COMBINED DONOR-RECIPIENT MANNOSE-BINDING LECTIN AND FICOLIN-2 GENE POLYMORPHISMS PREDISPOSE TO HUMAN CYTOMEGALOVIRUS INFECTION AFTER ORTHOTOPIC LIVER TRANSPLANTATION
Authors: B.-J.F. de Rooij, M.T. van der Beek, B. van Hoek, A.C.T.M. Vossen, W.R. ten Hove, A. Roos, A.F. Schaapherder, R.J. Porte, J.J. van der Reijden, M.J. Coenraad, D.W. Hommes, H.W. Verspaget
Journal: Journal of Hepatology (2011)
Reconstruction of perioral defects post-Mohs micrographic surgery: a dermatological surgeon's approach
Authors: J. Hafiji, W. Hussain, P. Salmon
Journal: British Journal of Dermatology (2014)

The perioral location has great functional, aesthetic and social importance. Over 100 procedures have been described for lip reconstruction, emphasizing the challenges that reconstructive surgeons face when dealing with such defects.To outline the surgical reconstruction techniques of perioral defects post-Mohs micrographic surgery (MMS).The design and surgical techniques of reconstruction of perioral defects post-MMS are described.The results of the surgical techniques described are illustrated.Herein, we describe the nuances of reconstructive techniques that we have found useful for repairing perioral defects post-MMS.
Tube Feeding and Pyriformsinusotomy
Authors: MALCOLM BLOCK
Journal: Annals of Internal Medicine (1979)

Letters and Corrections1 May 1979Tube Feeding and PyriformsinusotomyMALCOLM BLOCK, M.D., THOMAS J. CARMODY, M.D., J. TURNER CARSON, M.D.MALCOLM BLOCK, M.D.Search for more papers by this author, THOMAS J. CARMODY, M.D.Search for more papers by this author, J. TURNER CARSON, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-90-5-856_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: Heymsfield and colleagues in the January 1979 issue (1) have recently described various enteral modalities of alimentation. In addition to nasogastric enteral feeding tubes we have found the use of tube-feeding via the pyriformsinusotomy procedure described by Schumrick (2) to be useful. With swallowing abnormalities resulting from cerebrovascular accidents and other conditions, this procedure offers the advantages of reduced risk of aspiration and subsequent pneumonitis in long-term alimentation; also, because the tube may be removed between meals and reinserted with little difficulty, the complications inherent with long-term nasogastric intubation are avoided, that is, esophageal erosion, ulcers, tracheoesophageal...References1. HYMESFIELDBETHELANSLEYNIXONRUDMAN SRJDD: Enteral hyperalimentation: an alternative to central venous hyperalimentation. Ann Intern Med 90:63-71, 1979 LinkGoogle Scholar2. SHUMRICK D: Pyriformsinusotomy. Arch Surg 94:277-279, 1967 CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: The Good Samaritan Hospital and Health Center Dayton, OH 45406 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 May 1979Volume 90, Issue 5Page: 856-856KeywordsIntubationPneumonitisStrokeSwallowingUlcers Issue Published: 1 May 1979 PDF DownloadLoading ...
BACTERIAL TOXAEMIA AND CARDIAC PAIN
Authors: W. Gordon
Journal: BMJ (1927)

IED'LCAL wbhen a. faint greeni colour appeared at fifty seconids.This was the onil speitinieii w-hich gave ainy reactiou withl the pyramidon also, anid that a fainit olne.The diet oni this day wi-as frie(d bacon, roast mweat, green vegetables, hoiled potatoes, nad(i f riedl sausages." Contamination in cooking" iiglt possibly lhave been a factor,-but on subsequenit days thisi risk was at least as great, especially oni those days wlheni pickle(d herrinig wvas takeni, wlhich, as stated above, gives aii inmnmediate deep blue colour withl Gre(yei sen's test.Three outnices of raw micat fai 1Nd to give a positive result witn either test, wvhile tlei cooked ox-blood, which was taken in amount sufficlient to give a definite reactioni if uncooked (two ounces), also gave lno reaction in the stools.I'lTus it seems that meat, and especially underdone meat, ma-y cause a green coloration after thir-ty.seconids with Gregersen's test, and a positive result with the pyramidoii test.The important conclusion indicated is that a blue or blue-green colour with Gregerseni's test, obtained before
A Clinical Study of the Graves' Constitution and Its Relation to Thyroid Disease
Authors: CARL H. FORTUNE
Journal: Annals of Internal Medicine (1933)

Article1 January 1933A Clinical Study of the Graves' Constitution and Its Relation to Thyroid DiseaseCARL H. FORTUNE, A.B., M.D.CARL H. FORTUNE, A.B., M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-6-7-869 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptIt is not the purpose of this paper to discuss the relative merits of existing conceptions of thyroid disease, nor to add to such theories; but to present certain data—gathered and interpreted by the clinician—bearing on the constitutional background of hyperthyroidism as expounded by Warthin. From time to time mention has been made of the frequent association between thymic enlargement and thyroid disease, but Warthin's address entitled "The Constitutional Entity of Exophthalmic Goiter and So-Called Toxic Adenoma"1is the most comprehensive statement of the significance of this association and has given rise to the phrase "Graves' Constitution (Warthin)" which has...References1 WARTHIN AS: The constitutional entity of exophthalmic goiter and so-called toxic adenoma, ANN. INT. MED., 1928, ii, 553-570. Google Scholar2 POTTER EB: Persistent thymus in exophthalmic goiter, in Contributions to Medical Science. The Aldred Scott Warthin Anniversary Volume, George Wahr, Ann Arbor, 1927, pp. 205-220. Google Scholar3 MOSCHCOWITZ E: The nature of Graves' disease, Arch. Int. Med., 1930, xlvi, 610-629. CrossrefGoogle Scholar4 WARTHIN AS: The pathology of goiter, Proc. Inter-State Postgrad. Med. Assoc. of N. Am., 1929, 383-385. Google Scholar5 SIMPSON WM: Graves' constitution (Warthin), Am. Jr. Surg., 1929, vii, 22-26. CrossrefGoogle Scholar6 HELLWIG CA: Graves' constitution (Warthin), Surg., Gyn. and Obs., 1931, lii, 43-57. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Lexington, Kentucky*From the Department of Internal Medicine of the University of Michigan. Received for publication, March 2, 1932. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 January 1933Volume 6, Issue 7Page: 869-876KeywordsAdenomasHyperthyroidismThyroid diseases Issue Published: 1 January 1933 PDF downloadLoading ...
Bone marrow transplantation for autoimmune diseases
Authors: M. Potter, C. Black, A. Berger
Journal: BMJ (1999)

The cross fertilisation of ideas between different medical specialties means that traditional techniques from one field are beginning to find surprising roles in others. Bone marrow transplantation, for example, is becoming more sophisticated and safer, particularly since the advent of peripheral blood stem cell transplantation, and this is now being studied as a treatment for autoimmune diseases.1–3

Conventionally, long term immunosuppressive drugs are administered to control the autoimmune disease process, but these offer little in the way of a cure. Because autoimmunity is viewed as a failure of the immune system to protect against self reactivity, however, some have argued that by completely “resetting” the immune system, it might be possible to eradicate the autoimmune disease process altogether. People with both haematological malignancies and autoimmune diseases sometimes go into remission from both conditions after undergoing bone marrow transplantation. This incidental observation has prompted some haematologists to argue that such a reset of the immune system may be provoked by completely ablating …