tbuckley/cabot-search huggingface dataset:
139. Optimization of Electroporation Parameters for the Intramuscular Delivery of Plasmids in Pigs
Authors: Fanjam Andrianaivo, Michèle Lecocq, Simone Wattiaux-De Coninck, Robert Wattiaux, Michel Jadot
Journal: Molecular Therapy (2004)

The mechanism of gene transfer into hepatocytes by the hydrodynamics-based transfection procedure is not clearly understood. It has been shown that after an hydrodynamic injection, a large proportion of plasmid DNA remains intact in the liver where it is bound to plasma membrane and suggested that this DNA could be responsible of the efficiency of the transfection (Lecocq M et al, J of Gene Med, 5, 142-156, 2003). We have investigated the problem by giving to the mice an hydrodynamic injection of isotonic NaCl, followed at different time intervals by a conventional injection of DNA, cold or labelled with 35S, with cDNA of luciferase as a reporter gene. Then, we have determined the consequences of that dual injection on luciferase expression and on DNA uptake by the liver and its intracellular fate. By such experiments, it is possible to establish the time dependency of the induction of liver changes caused by an hydrodynamic injection on the one hand and the expression and DNA uptake and fate on the other hand.
Expression of apolipoprotein C-1 in pancreatic cancer and its role in cell survival by avoiding apoptotic cell death
Authors: Hideyuki Yoshitomi, Shigeaki Takano, Kazuyuki Sogawa, Fumio Kimura, Hiroaki Shimizu, Hiroyuki Yoshidome, Masayuki Otsuka, Takeshi Tomonaga, Fumio Nomura, Masaru Miyazaki
Journal: Clinical Cancer Research (2006)

B31  The final goal of this study is developing new therapy against pancreatic cancer. Despite the recent advances of clinical managements, pancreatic cancer still remains one of the most lethal diseases with increasing incidence. This suggests that establishment of new target therapy is needed. For this purpose, we tried to identify a new serum factor highly expressed in pancreatic cancer patients, and examine if this new factor involves in pancreatic cancer development.To identify new serum factor in pancreatic cancer patients, we compared pre- and postoperative serum protein profiling obtained from patients with invasive pancreatic ductal carcinoma. Samples from twenty patients who underwent curative pancreatectomy were analyzed and we successfully identified 6630 Da protein, which is Apolipoprotein C-1 (ApoC-1), as more abundantly expressed in preoperative serum than in postoperative serum (p
Lokal İleri Küçük Hücreli Dışı Akciğer Kanserli Hastalarda Torasik Radyoterapi ile Eş Zamanlı Pemetrekset ve Karboplatin veya Sisplatinin Kullanımının Toksisite ve Yanıt Değerlendirmesi
Authors: John H. Heinzerling, Hak Choy, Randall S. Hughes, Ramaswamy Govindan, Jeffrey D. Bradley, Lee S. Schwartzberg, Guangbin Peng, Joseph Treat, Taylor Tran, Bs, Coleman Obasaju
Journal: Journal of Thoracic Oncology (2010)
Diagnosis and Management of Breast Cancer.
Authors: Marc E. Lippman, Allen S. Lichter, David N. Danforth
Journal: Annals of Internal Medicine (1988)

This book contains 20 chapters. Some of the chapter titles are: Radiation Therapy as Definitive Treatment of Breast Cancer; Technical Aspects of the Treatment of Breast Cancer with Radiation Therapy; Radiation Therapy as an Adjuvant to Surgery in the Treatment of Operable Breast Cancer; Locally Advanced Breast Cancer; Special Situations in the Treatment of Breast Cancer; and Reconstruction of the Breast.
Impact of Long-Term Burden of Excessive Adiposity and Elevated Blood Pressure From Childhood on Adulthood Left Ventricular Remodeling Patterns
Authors: Chin-Chih Lai, Dianjianyi Sun, Ruiqi Cen, Jian Wang, Shengxu Li, Camilo Fernandez-Alonso, Wei Chen, Sathanur R. Srinivasan, Gerald S. Berenson
Journal: Journal of the American College of Cardiology (2014)
Mutation of a new gene causes a unique form of Hermansky–Pudlak syndrome in a genetic isolate of central Puerto Rico
Authors: Yair Anikster, Marjan Huizing, James White, Yuriy O. Shevchenko, Diana L. Fitzpatrick, Jeffrey W. Touchman, John G. Compton, Sherri J. Bale, Richard T. Swank, William A. Gahl, Jorge R. Toro
Journal: Nature Genetics (2001)
Abstract 14477: Medial Regeneration Using a Biodegradable Felt as a Scaffold Preserves Integrity and Compliance of a Canine Dissected Aorta
Authors: Mitsuru Sato, Osamu Adachi, Masatoshi Akiyama, Kiichiro Kumagai, Naotaka Motoyoshi, Shunsuke Kawamoto, Naoya Sakamoto, Masaaki Sato, Yasuhiko Tabata, Yoshikatsu Saiki
Journal: Circulation (2011)

Background: Repair of a dissected aorta consists of reattachment of the media with synthetic glue and/or reinforcement with a non-biodegradable felt. There have been reported late complications spe...
Abstract P426: Association of Serum Soy Isoflavones with Metabolic Markers in Japanese Men and Women
Authors: Rhobert W Evans, Akira Sekikawa, Takashi Kadowaki, Abhishek Vishnu, Sayaka Kadowaki, Aiman El-Saed, Akira Fujiyoshi, Vasudha Ahuja, Tomonori Okamura, Katsuyuki Miura, Lewis H Kuller, Hirotsugu Ueshima
Journal: Circulation (2014)

Objectives: Soy isoflavones may have beneficial roles as antioxidants and phytoestrogens. Dietary intake varies widely across populations: very high in Japan but low in North America. We measured the concentrations of daidzein and genistein in the serum of Japanese men and women and evaluated their associations with metabolic markers. Methods: Serum samples were obtained from a population based sample of 94 Japanese men (mean age 45.3), and 142 Japanese women (mean age 66.6; 97% were post-menopausal). Isoflavones were analyzed after extraction and derivatization using GC-MS. Concentrations of daidzein and genistein were summed to obtain total isoflavone concentrations. Results: Isoflavone concentrations were very high among the Japanese. Median values (nM) for men: 558, 452 and 86 and for women 660, 494 and 150 (total isoflavones, genistein and daidzein, respectively). Spearman correlation coefficients for the isoflavones with multiple outcomes among Japanese men and women are shown in the table. No significant (p&lt;0.05) associations were observed among the men but borderline negative associations were observed for total isoflavones (p=0.063) and genistein (p=0.063) with LDLc. Among women there was a significant association between total isoflavones (p=0.041) and daidzein (p=0.006) with HDLc. Borderline significant associations were also observed for total isoflavones (p=0.068) and genistein (p=0.061) with glucose. Discussion: The concentrations of isoflavones are high in the Japanese, approximately 50 times greater than those of N. Americans. Associations between the isoflavones and various outcomes were very weak for the Japanese men. This possibly reflects a plateau of isoflavone concentration has been reached that precludes observing any associations. Although, many correlations were calculated the positive associations of total isoflavones and daidzein with HDLc among women may be real. HDLc concentrations are high in Japanese women which may reflect the estrogenic actions of isoflavones.
Abstract 977: Characterizing the regulation and function of miR-155 in hypoxia biology
Authors: Jennifer Czochor, Peter M. Glazer
Journal: Cancer Research (2014)

Abstract Aberrant expression of microRNAs has been correlated with the initiation and progression of many diseases. One such microRNA, miR-155, is an oncogenic microRNA that has been associated with several different types of cancer, including lung, breast, and lymphoma. We are studying the role of miR-155 in promoting hypoxia-driven tumor phenotypes such as therapy resistance and genomic instability. Previously, we have shown that miR-155 expression is induced under hypoxic conditions and that over-expression of miR-155 under normal oxygen conditions confers resistance to ionizing radiation therapy. Additionally, we have shown that by knocking down expression of miR-155 under hypoxic conditions, we were able to sensitize these cells to ionizing radiation. Here, we demonstrate that miR-155 expression is tightly linked to genomic instability, another characteristic of hypoxia. We have found that over-expression of miR-155 drives increased mutation frequency as measured by a mutation reporter assay screen both in vitro and in vivo. These results are consistent with a recent finding that miR-155 over-expression targets mismatch repair proteins, MLH1, MSH2, and MSH6. An increase in mutation frequency could be due to a decrease in mismatch repair function. Surprisingly, we have also found that loss of miR-155 increases the baseline level of double strand breaks observed in a fibroblast cell line. Taken together, this data suggests that a finite regulation of miR-155 is necessary to maintain cellular homeostasis. Our goal is to gain a better understanding of the role of miR-155 in promoting hypoxic cancer cell survival. Citation Format: Jennifer Czochor, Peter M. Glazer. Characterizing the regulation and function of miR-155 in hypoxia biology. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 977. doi:10.1158/1538-7445.AM2014-977
Abstract 1716: Targeting the insulin-like growth factor receptor/Insulin receptor and Src signaling network for the treatment of non-small cell lung cancer
Authors: Hye-Young Min, Hye Jeong Yun, Hyo-Jong Lee, Jaebeom Cho, Hyun-Ji Jang, Kyung Min Kim, Woo-Young Kim, Seung-Hyun Oh, Diane Liu, J. Jack Lee, Waun K. Hong, Ignacio I. Wistuba, Ho-Young Lee
Journal: Cancer Research (2014)

Abstract Insulin-like growth factor I receptor (IGF-1R)-mediated signaling plays an important role in the proliferation, survival, and metastasis of cancer cells. The IGF-1R-targeting anticancer agents including monoclonal antibodies and small molecule tyrosine kinase inhibitors (TKIs) have been developed, but their antitumor effects have been marginal and limited in clinical trials. Therefore, the mechanism underlying resistance to the IGF-1R-targeting therapies and the rational combination strategies to overcome potential drug resistance need to be investigated. In this study, we demonstrated the association of Src with the resistance to the IGF-1R TKI in non-small cell lung cancer (NSCLC). We found the co-activation of IGF-1R/IR and Src in various human NSCLC cell lines, The mRNA expression and phosphorylation of IGF-1R and Src were also significantly correlated with each other in NSCLC databases from a public dataset and a tissue microarray (n=353). Next, we found Src can be activated through multiple pathways including EGFR and integrin β3 signaling and function as an alternative kinase for phosphorylation of IGF-1R, especially at Tyr1135/36, but not Tyr1131. Src is activated in lung cancer cells possessing both primary and acquired resistance to an IGF-1R TKI. Consistent with the results, inhibition of Src significantly attenuated activation of IGF-1R/IR and co-targeting of IGF-1R and Src effectively suppressed cell proliferation, colony formation, and tumor growth in vitro and in vivo. Taken together, these results suggest that Src causes resistance to IGF-1R TKIs by functioning as a key downstream modulator of multiple signaling pathways for IGF-1R phosphorylation and thus co-targeting IGF-1R and Src could be an effective therapeutic strategy for NSCLC. Citation Format: Hye-Young Min, Hye Jeong Yun, Hyo-Jong Lee, Jaebeom Cho, Hyun-Ji Jang, Kyung Min Kim, Woo-Young Kim, Seung-Hyun Oh, Diane Liu, J. Jack Lee, Waun Ki Hong, Ignacio I. Wistuba, Ho-Young Lee. Targeting the insulin-like growth factor receptor/Insulin receptor and Src signaling network for the treatment of non-small cell lung cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1716. doi:10.1158/1538-7445.AM2014-1716
Abstract 13541: Efficacy of Cilostazol After Femoropopliteal Nitinol Stenting
Authors: Yoshimitsu Soga, Osamu Iida, Keisuke Hirano, masakiyo Nobuyoshi
Journal: Circulation (2010)

Background: Restenosis after endovascular treatment of femoropopliteal (FP) artery remains a significant clinical problem. We investigated whether cilostazol would reduce restenosis in patients aft...
ARRHENOBLASTOMA OF THE OVARY: CASE REPORT AND REVIEW OF THE LITERATURE
Authors: F H BENTEEN, F M HEBERT, G C LAMBERT
Journal: Annals of Internal Medicine (1956)

Case Reports1 August 1956ARRHENOBLASTOMA OF THE OVARY: CASE REPORT AND REVIEW OF THE LITERATUREFREDERICK M. HEBERT, M.D., F.A.C.P., F. HARRY BENTEEN, M.D., GEORGE C. LAMBERT, M.D.FREDERICK M. HEBERT, M.D., F.A.C.P.Search for more papers by this author, F. HARRY BENTEEN, M.D.Search for more papers by this author, GEORGE C. LAMBERT, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-45-2-311 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptArrhenoblastoma of the ovary is a relatively rare tumor. Pick1 first described a well differentiated tumor of tubular structure found in the ovary and called it adenoma testiculare. This tumor was hormonally inactive. Meyer2 studied these tumors extensively and gave them their name, using the Greek word for male (arrhenos) as a prefix. In an extensive study published in 1947 Iverson3 reviewed 94 cases. She found the average age at onset in her review to be 32 years. It has not been known to occur in a patient younger than 16, and cases beyond the menopause are infrequent.Since 1947...Bibliography1. Soffer LJ: Diseases of the endocrine glands, 1951, Lea and Febiger, Philadelphia. Google Scholar2. Meyer R: Pathology of some special ovarian tumors and their relation to sex characteristics, Am. J. Obst. and Gynec. 22: 697, 1931. CrossrefGoogle Scholar3. Iverson L: Masculinizing tumors of the ovary, Surg., Gynec. and Obst. 84: 213, 1947. MedlineGoogle Scholar4. Novak E: Gynecological and obstetrical pathology, 1952, W. B. Saunders Co., Philadelphia. Google Scholar5. Geist SH: Ovarian tumors, 1942, Paul B. Hoeber, Inc., New York. Google Scholar6. Escamilla RF: Laboratory aids in endocrine diagnosis, 1954, Charles C. Thomas, Springfield, Illinois. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: *Received for publication May 23, 1955.Requests for reprints should be addressed to Frederick M. Hebert, M.D., 2640 Telegraph Avenue, Berkeley 4, California. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByEndocrine studies in an arrhenoblastoma responsive to dexamethasone, ACTH and human chorionic gonadotropinA clinical analysis of ovarian virilizing tumorsArrhenoblastoma of the ovaryArrhenoblastoma of the ovary 1 August 1956Volume 45, Issue 2Page: 311-318KeywordsAdenomasDifferentiated tumorsHormonesMenopauseOvariesTestes Issue Published: 1 August 1956 PDF DownloadLoading ...
THE CHANGING ORDER IN MEDICINE
Authors: James Alexander Miller
Journal: Annals of Internal Medicine (1936)

Article1 April 1936THE CHANGING ORDER IN MEDICINEJAMES ALEXANDER MILLER, A.M., M.D., F.A.C.P.JAMES ALEXANDER MILLER, A.M., M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-9-10-1287 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptI would be very neglectful of my opportunity and very untrue to my deep feelings if I failed upon this occasion to record my great appreciation of the progress the American College of Physicians has made and is making in the rôle of leadership in the field of internal medicine, and also my very deep and heartfelt appreciation of the privilege and honor which has been conferred upon me in my selection to preside over the activities of the College during the past year.College Matters. This year has been fruitful in accomplishments. The affairs of the College have continued... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: New York, N. Y.*Presidential Address, delivered at the Convocation Exercises of the American College of Physicians, Twentieth Annual Session, Detroit, March 4, 1936. Nextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byAlan Gregg and the rise of general hospital psychiatry 1 April 1936Volume 9, Issue 10Page: 1287-1302KeywordsEmotionsExercise ePublished: 1 December 2008 Issue Published: 1 April 1936 PDF downloadLoading ...
HLA-DR15 (DR2) is overrepresented in myelodysplastic syndrome and aplastic anemia and predicts a response to immunosuppression in myelodysplastic syndrome
Authors: Yogen Saunthararajah, Ryotaro Nakamura, Jun-Mo Nam, Jamie Robyn, Fausto Loberiza, Jaroslaw P. Maciejewski, Toni Simonis, Jeffrey Molldrem, Neal S. Young, A. John Barrett
Journal: Blood (2002)
Hyperfractionated Low-Dose Radiotherapy for High-Risk Neuroblastoma After Intensive Chemotherapy and Surgery
Authors: Brian H. Kushner, Suzanne Wolden, Michael P. LaQuaglia, Kim Kramer, David Verbel, Glenn Heller, Nai-Kong V. Cheung
Journal: Journal of Clinical Oncology (2001)

PURPOSE: To assess prognostic factors for local control in high-risk neuroblastoma patients treated with hyperfractionated 21-Gy total dose to consolidate remission achieved by dose-intensive chemotherapy and surgery. PATIENTS AND METHODS: Patients with high-risk neuroblastoma in first remission received local radiotherapy (RT) totaling 21 Gy in twice-daily 1.5-Gy fractions. RT to the primary site followed dose-intensive chemotherapy and tumor resection; the target field encompassed the extent of tumor at diagnosis, plus 3-cm margins and regional lymph nodes. RT to distant sites followed radiologic evidence of response. Local failure was correlated with clinical factors (including other consolidative treatments) and biologic findings. RESULTS: Of 99 consecutively irradiated patients followed for a median of 21.1 months from RT, 10 relapsed in or at margins of RT fields at 1 to 27 months (median, 14 months). At 36 months after RT, the probability of primary-site failure was 10.1% ± 5.3%. No primary-site relapses occurred among the 23 patients whose tumors were excised at diagnosis, but there were three such relapses among the seven patients who were irradiated with evidence of residual disease in the primary site. Four of 18 patients with MYCN-amplified disease and serum lactate dehydrogenase greater than 1,500 U/L had local failures (23.4% ± 10.7% risk at 18 months). Acute radiotoxicities were insignificant, but three of 35 patients followed for ≥ 36 months had short stature from decreased growth of irradiated vertebra. CONCLUSION: Hyperfractionated 21-Gy RT is well tolerated and, together with dose-intensive chemotherapy and surgery, may help in local control of high-risk neuroblastoma. Extending the RT field to definitively encompass regional nodal groups may improve results. Visible residual disease may warrant higher RT dosing. Patients with biologically unfavorable disease may be at increased risk for local failure. RT to the primary site may not be necessary when tumors are excised at diagnosis.
Abstract 3046: Lin28B mediated IKK-β sustains the stemness of breast cancer stem cell via regulating Wnt/TCF4 and miR-34a/LEF1 signaling pathway
Authors: Chong Chen, Lipeng Bai, Fengqi Cao, Yan Liu, Junling Xie, Wei Wang, Qin Si, Jian Yang, Antao Chang, Rong Xiang, Yunping Luo
Journal: Cancer Research (2014)

Abstract Substantial evidence indicates that many human cancers are driven by a subpopulation of cells that display stem cell properties. These cancer stem cells (CSCs) may also contribute to tumor metastasis and treatment resistance. It has been reported that inflammatory factors in tumor microenvironment activate NF-kB/IKK-β pathway in tumor cells which in turn drive CSC self-renewal, but their molecular basis remain unclear. In this study, we demonstrated a novel mechanism regarding that Lin28B, a RNA binding protein regulated by IKK-β, sustains the stemness of breast cancer stem cell via regulating Wnt/TCF4 and miR-34a/LEF1 signaling pathway. First, we found that all stemness properties of MDA-MB-231 human breast cancer cells including ALDH+ population, Sphere-forming ability and the expression of stemness genes SOX2, OCT4, NANOG and Lin28B were decreased by IKK-β inhibition, meanwhile, the down regulated expression of Lin28B decrease the stemness properties of MDA-MB-231 cells as well. Furthermore, it was found that Wnt/TCF4 signaling pathway was inhibited by Lin28B down-regulation via reducing the expression of TCF4. Interestingly, TCF4 could also regulate the expression of Lin28B by directly binding to the intron 1 of Lin28B. Moreover, we observed that microRNA-34a was down regulated by Lin28B at transcriptionally level, and increased miR34a expression inhibited the activity of Wnt/LEF1 pathway by directly targeting LEF1. Most importantly, we found that down regulated expression of Lin28B or IKK-β inhibition combined with chemo-drug (IKK-β selective inhibitor IMD-0354 combine with paclitaxel) could markedly suppressed tumor growth and metastasis of MDA-MB-231 breast tumor cells in NOD/SCID mice model. Taken together, our study demonstrated that Lin28B, which was activated by IKK-β, could sustain the stemness of CSC via the TCF4 and miR-34a/LEF1 signaling regulation, thus, establish the Lin28B as a central regulator in govern inflammation to maintain breast cancer stemness phenotype, and could serve as a therapeutical target. Citation Format: Chong Chen, Lipeng Bai, Fengqi Cao, Yan Liu, Junling Xie, Wei Wang, Qin Si, Jian Yang, Antao Chang, Rong Xiang, Yunping Luo. Lin28B mediated IKK-β sustains the stemness of breast cancer stem cell via regulating Wnt/TCF4 and miR-34a/LEF1 signaling pathway. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3046. doi:10.1158/1538-7445.AM2014-3046