QUANTITATIVE STUDIES ON INCREASED POTENCY OF LIVER EXTRACT BY INCUBATION WITH NORMAL HUMAN GASTRIC JUICE
Authors: Paul J. Fouts, O. M. Helmer, L. G. Zerfas
Journal: Annals of Internal Medicine (1935)

Article1 January 1935QUANTITATIVE STUDIES ON INCREASED POTENCY OF LIVER EXTRACT BY INCUBATION WITH NORMAL HUMAN GASTRIC JUICEPAUL J. FOUTS, M.D., O. M. HELMER, PH.D., L. G. ZERFAS, M.D., F.A.C.P.PAUL J. FOUTS, M.D.Search for more papers by this author, O. M. HELMER, PH.D.Search for more papers by this author, L. G. ZERFAS, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-8-7-790 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe therapeutic effectiveness of liver and fractions of liver in the treatment of pernicious anemia is well established. Minot, Cohn, Murphy, and Lawson1demonstrated that although slight reticulocyte responses followed the daily feeding of 60 grams of cooked liver, near-maximal responses did not usually occur unless from 150 to 200 grams of cooked liver were fed. Minot, Murphy, and Stetson2stated that: "There is apparently some minimal amount of liver in the vicinity of 60 gm. of cooked liver, which is a necessary daily amount to produce a distinct reticulocyte reaction when the red blood cells are about 1.25 million...Bibliography1. MINOTCOHNMURPHYLAWSON GREJWPHA: Treatment of pernicious anemia with liver extract, Am. Jr. Med. Sci., 1928, clxxv, 599-622. CrossrefGoogle Scholar2. MINOTMURPHYSTETSON GRWPRP: The response of the reticulocytes to liver therapy, Am. Jr. Med. Sci., 1928, clxxv, 581-599. CrossrefGoogle Scholar3. ZERFAS LG: Liver extract for pernicious anemia: blood changes during the first month; report of 101 cases, Arch. Int. Med., 1931, xlvii, 135-143. CrossrefGoogle Scholar4. CASTLE WB: Observations on the etiologic relationship of achylia gastrica to pernicious anemia. I. The effect of the administration in patients with pernicious anemia of the contents of the normal human stomach recovered after the ingestion of beef muscle, Am. Jr. Med. Sci., 1929, clxxviii, 748-764. CASTLE, W. B., and TOWNSEND, W. C.: Ibid. II. The effect of the administration to patients with pernicious anemia of beef muscle after incubation with normal human gastric juice, Am. Jr. Med. Sci., 1929, clxxviii, 764-777. CASTLE, W. B., TOWNSEND, W. C., and HEATH, C. W.: Ibid. III. The nature of the reactions between normal human gastric juice and beef muscle leading to clinical improvement and increased blood formation similar to the effect of liver feeding, Am. Jr. Med. Sci., 1930, clxxx, 305-335. CASTLE, W. B., HEATH, C. W., and STRAUSS, M. B.: Ibid. IV. A biologic assay of the gastric secretion of patients with pernicious anemia having free hydrochloric acid and that of patients without anemia or with hypochromic anemia having no free hydrochloric acid, and of the rôle of intestinal impermeability to hematopoietic substances in pernicious anemia, Am. Jr. Med. Sci., 1931, clxxxii, 741-764. STRAUSS, M. B., and CASTLE, W. B.: The nature of the extrinsic factor of the deficiency state in pernicious anemia and in related macrocytic anemias. Activation of yeast derivatives with normal human gastric juice, New Eng. Jr. Med., 1932, ccvii, 55-59. CrossrefGoogle Scholar5. SHARP EA: An antianemic factor in desiccated stomach, Jr. Am. Med. Assoc., 1929, xciii, 749. CrossrefGoogle Scholar6. STURGISISAACS CCR: Desiccated stomach in the treatment of pernicious anemia, Jr. Am. Med. Assoc., 1929, xciii, 747-749. CrossrefGoogle Scholar7. CONNER HM: The feeding of gastric tissue in treatment of pernicious anemia, Jr. Am. Med. 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HELMERFOUTSZERFAS OMPJLG: Increased potency of liver extract by incubation with human gastric juice, Proc. Soc. Exper. Biol. and Med., 1933, xxx, 775-778. CrossrefGoogle Scholar13. HELMERFOUTSZERFAS OMPJLG: The relationship of the intrinsic factor to a hematopoietic material in concentrated human gastric juice, Am. Jr. Med. Sci., 1934, clxxxviii, 184-193. CrossrefGoogle Scholar14. HARTFALLWITTS SJLJ: The intrinsic factor of Castle in simple achlorhydric anemia, Guy's Hosp. Rep., 1933, lxxxiii, 24-36. Google Scholar15. SPIESPAYNE TDW: A study of the etiological relationship between pellagra and pernicious anemia, Jr. Clin. Invest., 1933, xii, 229-234. CrossrefGoogle Scholar16. BEEBEWINTROBE RTMM: Diagnosis of obscure cases of pernicious anemia, Arch. Int. Med., 1933, li, 630-637. CrossrefGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Indianapolis, Indiana*Received for publication July 18, 1934.From the Lilly Laboratory for Clinical Research, Indianapolis City Hospital, and the Department of Medicine, Indiana University School of Medicine. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byThe Role of Vitamin B12 in Metabolic ProcessesObservations on the Etiologic Relationship of Achylia Gastrica to Pernicious AnemiaPhysiology of Anti-Pernicious Anemia MaterialFURTHER OBSERVATIONS ON THE CHEMICAL NATURE OF A HEMATOPOIETIC SUBSTANCE OCCURRING IN LIVERAn investigation into the production of a proteolytic ferment in the duodenum which will increase the anti-anemic efficacy of liver; its relationship to the cause of pernicious anemia 1 January 1935Volume 8, Issue 7Page: 790-797KeywordsClinical laboratoriesHospital medicineLiverPernicious anemiaRed blood cellsResearch laboratoriesReticulocytes ePublished: 1 December 2008 Issue Published: 1 January 1935 PDF downloadLoading ...