Important Differences in Measurement of Fetuin-A
Authors: Edward R. Smith
Journal: Annals of Internal Medicine (2010)

Letters21 September 2010Important Differences in Measurement of Fetuin-AEdward R. Smith, MSc, PhD and Stephen G. Holt, MBBS, PhDEdward R. Smith, MSc, PhDFrom Brighton and Sussex University Hospitals NHS Trust, Brighton BN2 5BE, United Kingdom. and Stephen G. Holt, MBBS, PhDFrom Brighton and Sussex University Hospitals NHS Trust, Brighton BN2 5BE, United Kingdom.Author, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-153-6-201009210-00016 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:Parker and colleagues (1) found no significant association between fetuin-A levels and mortality in a cohort with prevalent coronary artery disease. Our understanding of fetuin-A biology is greatly undermined by the lack of agreement among different methods of measurement. It is therefore particularly important that method descriptions are unambiguous. The authors comment that their nephelometric fetuin-A assay "uses the same high-specificity antibody as commercially available enzyme-linked immunosorbent assays." It is unclear to which commercially available assays they are referring—the 2 most widely used commercial kits not only use different antisera but also use a sandwich format with ...References1. Parker BD, Schurgers LJ, Brandenburg VM, Christenson RH, Vermeer C, Ketteler M, et al. The associations of fibroblast growth factor 23 and uncarboxylated matrix Gla protein with mortality in coronary artery disease: the Heart and Soul Study. Ann Intern Med. 2010;152:640-8. [PMID: 20479029] LinkGoogle Scholar2. Smith ER, Ford ML, Tomlinson LA, Rocks BF, Rajkumar C, Holt SG. Poor agreement between commercial ELISAs for plasma fetuin-A: an effect of protein glycosylation? Clin Chim Acta. 2010;411:1367-70. [PMID: 20420817] CrossrefMedlineGoogle Scholar3. Hermans MM, Brandenburg V, Ketteler M, Kooman JP, van der Sande FM, Boeschoten EW, et al; Netherlands cooperative study on the adequacy of Dialysis (NECOSAD). Association of serum fetuin-A levels with mortality in dialysis patients. Kidney Int. 2007;72:202-7. [PMID: 17342178] CrossrefMedlineGoogle Scholar4. Ford ML, Tomlinson LA, Smith ER, Rajkumar C, Holt SG. Fetuin-A is an independent determinant of change of aortic stiffness over 1 year in non-diabetic patients with CKD stages 3 and 4. Nephrol Dial Transplant. 2010;25:1853-8. [PMID: 20075438] CrossrefMedlineGoogle Scholar Author, Article, and Disclosure InformationAffiliations: From Brighton and Sussex University Hospitals NHS Trust, Brighton BN2 5BE, United Kingdom.Disclosures: None disclosed. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoThe Associations of Fibroblast Growth Factor 23 and Uncarboxylated Matrix Gla Protein With Mortality in Coronary Artery Disease: The Heart and Soul Study Benjamin D. Parker , Leon J. Schurgers , Vincent M. Brandenburg , Robert H. Christenson , Cees Vermeer , Markus Ketteler , Michael G. Shlipak , Mary A. Whooley , and Joachim H. Ix Important Differences in Measurement of Fetuin-A Vincent M. Brandenburg , Benjamin D. Parker , and Joachim H. Ix Metrics Cited ByThe Isolation and Quantitation of Fetuin-A-Containing Calciprotein Particles from Biological FluidsUsefulness of Fetuin-A to Predict Risk for Cardiovascular Disease Among Patients With Obstructive Sleep ApneaRelationship between sclerostin and cardiovascular calcification in hemodialysis patients: a cross-sectional studyCerebrospinal fluid fetuin-A is a biomarker of active multiple sclerosisSerum fetuin-A concentration and fetuin-A-containing calciprotein particles in patients with chronic inflammatory disease and renal failurePhosphorylated fetuin-A-containing calciprotein particles are associated with aortic stiffness and a procalcific milieu in patients with pre-dialysis CKDFetuin-A and the cardiovascular system 21 September 2010Volume 153, Issue 6Page: 419KeywordsChronic kidney diseaseConflicts of interestEnzyme linked immunosorbent assayProteinsRenal diseasesSpecificityStable coronary artery diseaseStiffness ePublished: 21 September 2010 Issue Published: 21 September 2010 CopyrightCopyright © 2010 by American College of Physicians. All Rights Reserved.PDF DownloadLoading ...