tbuckley/cabot-search huggingface dataset:
Abstract P225: Association Between Perfluoralkyl Acids and Self-Reported Stroke or Cerebral Vascular Accident
Authors: Stephanie J Frisbee
Journal: Circulation (2014)

PFCs are a class of man-made compounds developed after WWII for use as plasticizers, wetting agents, and as emulsifiers during industrial manufacturing processes. Several lines of research suggest a direct and indirect role for PFCs in stroke and stroke outcome, including: detection in human brain tissue and cerebrospinal fluid and relationships to conditions known to affect stroke, including: homocysteine and blood pressure, thyroid function; immune function, and hypercholesterolemia. The C8 Health Project resulted from a pre-trial settlement of the class action lawsuit filed after PFOA was found to have infiltrated several local drinking water supplies along the mid-Ohio River valley. Final enrollment totaled 69,030 participants. We tested the hypothesis that PFCs were associated with self-reported stroke or cerebral vascular accident. The primary independent variables of interest were the serum concentrations (ng/mL) of 7 different PFCs (PFHxA, PFHS, PFHpA, PFOA, PFOS, PFNA, PFDA), partitioned into age and gender-specific quintiles. Covariates included in this analyses were: demographic; lifestyle / modifiable risk factors; and medical risk factors. We did not include race as a covariate (≥97% of C8 Health Project participants self-identified as white). The primary dependent variable of interest was self-reported stroke or cerebral vascular accident. In univariate analysis (ANOVA), PFHpA and PFOS levels were higher in participants with Stroke/CVA, but PFNA was lower in participants with Stroke/CVA. Results from logistic regression analysis are presented. For four of seven chemicals included in the analysis, there were statistically significant associations with prevalent Stroke/CVA. PFHxA was associated with increased prevalence of Stroke/CVA, whereas PFHS, PFOS, and PFNA were associated with a decreased prevalence of Stroke/CVA. Thus, results from this preliminary analysis support an associations between PFCs and prevalent Stroke/CVA that warrant further, more rigorous investigation.
In Europe, as in US, Climate for Embryo Stem Cell Research Is One of Extremes
Authors: Xavier Bosch
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
146. Golden Cells: Gold Conjugate Labeling as a Potential Enhancer of Electro-Gene Transfer
Authors: Feng Liu, C. E. Polley, L. B. Stiger, S. Majetich, Leaf Huang
Journal: Molecular Therapy (2004)

When cells undergo electroporation, electric pulses cause a reversible breakdown of the membrane, allowing the entry of DNA. Electroporation can be performed on quiescent cells and there is litter or no risk of insertional mutagenesis. However, mechanical damage to cells makes the process less efficient at non-toxic conditions. Colloidal gold particles are electron-dense, negatively charged spheres that exist as monodisperse suspensions due to hydrostatic repulsions. They are further stabilized when halos of protein are non-covalently adsorbed to the particles, thus a receptor-specific gold conjugate can be formed. Since the gold particles are conductive metals, the electric field strength will be more dense in the region surrounding the gold particles. Theoretically, electro-gene transfer should be enhanced in cells labeled with sufficiently large gold particles in close proximity to the cell membrane. To investigate this hypothesis, Hela cells were labeled with gold particles conjugated to transferrin receptor (TfR)-targeting proteins. First, a mouse monoclonal anti-human TfR and a sheep anti-mouse IgG conjugated to FITC were bound to Hela cells. Fluorescence of cell surface confirmed the presence of the transferrin receptor. Then, the primary TfR-recognizing antibody was bound to a 6nm immunogold particle coated with goat anti-mouse IgG. Luciferase plasmid was introduced into the cells using a T820 ElectroSquarePoratorTM. The presence of gold particles on the cells was confirmed using an Aurion Silver Enhancement Reagent. Under these conditions, no significant difference was noted between the control and gold labeled cells. Assuming the gold particle was too small or too far away from the membrane to effect electro-gene transfer, we made two adjustments to the protocol. First, the experiment was conducted with 25nm immunogold particles with goat anti-mouse IgG. Second, the electroporation was conducted with 25nm gold particles conjugated directly to holo-transferrin protein, thus decreasing the distance between cell and gold particle. The indirect conjugation using the 25nm gold particle did not significantly increase transfection efficiency. However, the cells with the gold-holo-transferrin conjugate showed significantly higher luciferase activity relative to the control group after 24 hours (95%CI, P<0.015). These results indicate that direct gold particle attachment is a promising method for increasing the efficiency of electro-gene transfer.
Scientists discover possible test for schizophrenia
Authors: Judy Siegel-Itzkovich
Journal: BMJ (2001)

Israeli researchers may have found a way to diagnose schizophrenia by analysing white blood cells for signs of a chemical that is overactive in patients with the psychiatric condition.

Psychiatrists may be able to give patients a simple blood test to determine at an early stage whether a patient has the disorder instead of observing behaviour for at least six months before diagnosing and treating it.

The blood test, which has been patented but is not likely to be commercially available for several years, was proposed and tried on patients by Professor Sara Fuchs of the immunology department of the Weizmann Institute of Science in Rehovot and by graduate student Tal Ilani.

Their study, carried out with help from colleagues at the Rambam Hospital in Haifa and the nearby Be'er Ya'acov and Tirat Hacarmel mental health …
Scoliosis. Rotary Lateral Curvature of the Spine.
Authors: Merrill N. Foote
Journal: JAMA (1926)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 508: A Reactive Oxygen Species-mediated PKC-ERK-RSK Pathway Decreases The Cyclic AMP Response Element Binding Protein And May Initiate Transcriptional Changes In Cardiac Memory
Authors: Nazira Ozgen, Alexei N Plotnikov, Iryna N Shlapakova, Peter Danilo, Susan F Steinberg, Michael R Rosen
Journal: Circulation (2007)

Introduction: We previously reported that ventricular pacing (VP) to induce cardiac memory (CM) results in a decreased outward K current Ito and reduced mRNA and protein of the Ito subunit, KChIP2....
Abstract 1926: Identification of stem cell by immunohistochemistry in benign and malignant human esophageal mucosa
Authors: Kim Vaiphei, Saroj Kant Sinha, Rakesh Kochhar
Journal: Cancer Research (2014)

Abstract Introduction: Esophageal squamous cell carcinoma (ESCC) response to treatment if still unsatisfactory with high mortality rate. Objective: to identify the role of cancer stem cell in ESCC by studying the tumor by immunohistochemistry, with the concept of identifying the possible cell for the progression of the tumor due to its uncontrolled proliferation. Materials and methods: endoscopic biopsy and resected specimens of confirmed ESCC tissue were used for the study - fresh tumor tissue with no treatment, post treated residual tumor tissue, recurrent tumor tissue and adjoining esophageal mucosa. Primary immunomarkers used in the study were : i) alpha 6 integrin, ii) CD71, iii) p63, iv) stem cell antigen1, v) cytokeratin 6, vi) Bim1, vii) oct4, viii) nucleostemin, ix) Ki67, x). CD133, x) CD44, xi) CD24 and xii) Aldehyde dehydrogenase. Study was done routine immunohistochemistry, double immunostaining and tissue micro array. Apropriate detection system was used. Results: There was 250 tumor tissue altogether majority with well differentiated morphology. All tumors showed variable range of positivity for the all the markers, lesser number of positive cells for CD24, CD44 and CD133. Even in double immunostaining, majority of the tumor cells showed co-expressions for the various immunomarkers. Different tumor samples from different patient population groups do not any significant differences. Mucosal dysplastic samples also showed similar pattern of positive staining as by the tumor cells. Ki67 posivity was variable, higher percentage of positivity in poorly differentiated tumor areas and related with the nucleostemin positive staining pattern. Conclusion: These observations indicate that these markers are non-specific and positivity does not specifically meant the presence of cancer stem cell. Possibly expression of these markers may indicate cell proliferative pathway markers. Note: This abstract was not presented at the meeting. Citation Format: Kim Vaiphei, Saroj Kant Sinha, Rakesh Kochhar. Identification of stem cell by immunohistochemistry in benign and malignant human esophageal mucosa. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1926. doi:10.1158/1538-7445.AM2014-1926
Abstract P364: Genetic Susceptibility to Particulate Matter-Associated QT Prolongation in a Trans-Ethnic Genome-Wide Association Study
Authors: Rahul Gondalia
Journal: Circulation (2015)

Background:  QT prolongation_an electrocardiographic risk factor for ventricular arrhythmia and sudden cardiac death_has both a genetic and environmental etiology. However, genetic susceptibility to particulate matter-associated QT interval (QT) prolongation has not been characterized.   Methods:  To characterize such susceptibility in a genome-wide association study, we conducted cohort-, race/ethnicity-, and sex-stratified longitudinal analyses of eight Women’s Health Initiative clinical trials (WHI CT) and Atherosclerosis Risk in Communities study (ARIC) subpopulations (total n: 20,595; mean age: 63.4 yrs; 77% female; 28% black; mean visits/person: 3.0). In each subpopulation, we used generalized estimating equation (GEE) methods adapted for small sample size to estimate interactive effects of dichotomized daily mean concentrations of ambient particulate matter   subpopulation-specific 90th percentiles) and approximately 2.5 million imputed single nucleotide polymorphisms (SNPs), while adjusting for age, geographic region or center, season, calendar year, RR(ms) and ancestral admixture. Subpopulation-specific SNP-PM10 interaction effects were meta-analyzed using fixed-effects, inverse variance-weighted, meta-analysis with genomic control.   Results:  A common variant (rs1619661; minor allele frequency range: 9% - 20%) was associated with QT prolongation in seven (88%) of the eight subpopulations (overall  P  = 8.2 x 10-8). The variant is on chromosome 10, 150 kb downstream from  CXCL12 , which encodes a chemokine stromal-cell derived factor 1 (SDF-1) that is expressed in cardiac myocytes, affects calcium homeostasis, and has an established role in both diabetogenesis and atherosclerosis. QT increased 2.6 and 0.0 ms per unit increase in dosage of the variant at PM10 concentrations > and ≤ the 90th percentile, respectively.   Conclusions:  The preliminary findings from this longitudinal genome-wide association study suggest that biologically plausible genetic factors may affect susceptibility to PM10-associated QT prolongation in racially, geographically and environmentally diverse populations protected by U.S. EPA National Ambient Air Quality Standards.has both a genetic and environmental etiology. However, genetic susceptibility to particulate matter-associated QT interval (QT) prolongation has not been characterized.
Biventricular Assist Device Utilization for Patients with Morbid Congestive Heart Failure
Authors: Hiroyuki Tsukui, Jeffrey J. Teuteberg, Srinivas Murali, Dennis M. McNamara, Julianne R. Buchanan, Stephen Winowich, Eileen Stanford, Michael A. Mathier, Linda M. Cadaret, Robert L. Kormos
Journal: Circulation (2005)

Background— The rationale for the use of a biventricular assist device (BiVAD) for morbid congestive heart failure (MCHF) has been questioned because of historically unacceptable rates of postimplant and post-transplant mortality as well as perceived barriers to their outpatient management. Methods and Results— All patients who received a Thoratec BiVAD from January 1990 to December 2003 at the University of Pittsburgh were studied retrospectively. There were a total of 73 patients (32% ischemic, 21% idiopathic, and 47% other) who had a BiVAD implanted. Before implantation, 100% were on ≥1 inotropic agent, and 77% had an intra-aortic balloon pump. Overall survival was 69%; 42 patients (84%) received cardiac transplantation, 5 patients (10%) were weaned, and 3 (6%) remained supported on BiVAD. If the 14 patients with postcardiotomy failure and acute myocardial infarction with shock are excluded, the overall survival improves to 75%. Five-year actuarial survival after heart transplantation was 58%. Of the 29 patients implanted before 2000, the 4-month actuarial freedom from driveline infections, bloodstream infections, and neurological events was 10%, 54%, and 48%, respectively, whereas the rates of these events for the 44 patients implanted after 2000 improved to 70%, 79%, and 80%, respectively. Since 2000, 21 (48%) patients were discharged from the hospital, of whom 38% went to an outpatient residence, 33% to a skilled nursing facility, and 29% to home. Once discharged, ≥1 readmission occurred in 45% and ≥2 readmissions in 48%. Conclusions— BiVAD support for MCHF has an acceptable overall mortality and survival to transplantation. Morbidity has been significantly reduced in the past 4 years, and management as an outpatient is achievable.
TUBERCULOUS AXILLARY ADENITIS FOLLOWING APPLICATION OF P.P.D.
Authors: F BRANCATO, R H LINN, J J WALSH
Journal: Annals of Internal Medicine (1956)

Case Reports1 September 1956TUBERCULOUS AXILLARY ADENITIS FOLLOWING APPLICATION OF P.P.D.JOHN J. WALSH, M.D., RICHARD H. LINN, M.D., FRANK BRANCATO, Ph.D.JOHN J. WALSH, M.D.Search for more papers by this author, RICHARD H. LINN, M.D.Search for more papers by this author, FRANK BRANCATO, Ph.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-45-3-547 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptMuch has been written of the clinical application of skin testing with purified protein derivative (P.P.D.) tuberculin, and the ramifications thereof. However, limited examination of the literature fails to reveal the report of a case of axillary tuberculous adenitis subsequent to skin testing with P.P.D. For this reason the following case is reported in its entirety.CASE REPORTThis 50 year old Negro seaman was admitted to the U. S. Public Health Service Hospital, Seattle, Washington, July 31, 1953, with the chief complaint of "a painful knot in my right armpit" of 24 days' duration. On June 3, 1953, routine... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: *Received for publication May 25, 1955.Requests for reprints should be addressed to John J. Walsh, M.D., Senior Surgeon, USPHS, U. S. Public Health Service Hospital, 210 State Street, New Orleans 18, Louisiana. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byA Characterization of Atypical Acid-Fast Bacilli Obtained from Patients with Pulmonary Tuberculosis 1 September 1956Volume 45, Issue 3Page: 547-549KeywordsInoculationMedical servicesMycobacterium tuberculosisPathogensProteinsTuberculin ePublished: 1 December 2008 Issue Published: 1 September 1956 PDF downloadLoading ...
Abstract 2961: Sirolimus affects Cardiomyocytes to Reduce Left Ventricular Mass in Heart Transplant Recipients
Authors: Sudhir S Kushwaha, Eugenia Raichlin, Yuri Sheinin, Walter K Kremers, Krishnaswamy Chandrasekaran, Gregory J Brunn, Jeffrey L Platt
Journal: Circulation (2008)

Background: Cardiac hypertrophy compromises cardiac function and decreases survival. in coronary disease, hypertension and following cardiac transplantation. The cellular mechanisms underlying card...
Important Warning About Alosetron Hydrochloride
Authors: Jane E. Henney
Journal: JAMA (2000)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 3299: Association Of The Metabolic Syndrome, Diabetes And Framingham Risk Score With Coronary Artery Calcium
Authors: Uzoma N Ibebuogu, nathan D Wong, Jessica Ramirez, SongShou Mao, Fereshteh Hajsadeghi, Ambarish gopal, Ferdinand R flores, Matthew J Budoff
Journal: Circulation (2007)

INTRODUCTION: Coronary artery calcium (CAC) is a sensitive marker for the detection of subclinical coronary heart disease (CHD), and can be accurately quantified using cardiac computed tomography. Few studies have examined the relation between the metabolic syndrome (MetS), MetS risk factor burden, diabetes, and CAC. HYPOTHESIS: To examine the relation between MetS, as defined by the National Cholesterol Education Program Adult Treatment Panel III (NCEP ATP III), MetS risk factor burden, diabetes and CAC. METHODS: We studied 356 consecutive, asymptomatic men and women aged 58 ± 11 years who underwent CAC testing, 116 had MetS, 61 had diabetes, and the remainder had neither. MetS was defined according to the NCEP ATP III guidelines with ≥ 3 risk factors. The prevalence and odds of CAC among these groups were determined by multivariable logistic regression analysis. Receiver operating characteristic curves were used to determine if MetS or diabetes added to 10-year CHD estimated by the Framingham risk score (FRS) in predicting CAC. RESULTS: The prevalence of CAC &gt;0 for those with diabetes, MetS and neither condition was 73%, 69% and 60% respectively, while the prevalence of CAC ≥ 100 for the 3 groups was 64%, 43% and 24% respectively. Risk factor-adjusted odds for the presence of CAC ≥ 100 were 2.26 (95% CI 1 to 4.4, p=0.0001) among those with MetS and 3.46 (95% CI 1.6 to 7.4, p=0.0001) among those with diabetes, versus neither condition. ROC analysis for CAC ≥ 100 showed an area under the curve of 0.61 (95% CI 0.54 – 0.68) for FRS, 0.72 (95% CI 0.61– 0.83) for diabetes, 0.67 (95% CI 0.56 – 0.77) for the metabolic syndrome, 0.78 (95% CI 0.7– 0.85) when the MetS is added to the FRS (p&lt;0.0001 compared to FRS alone), and 0.90 (95% CI 0.85– 0.95) when diabetes is added to the FRS (p&lt;0.0001 compared to FRS alone). The CAC score showed a trend towards a progressive increase across the metabolic score ranging from 0 to 5 (p=0.0001). CONCLUSIONS: Those with MetS or diabetes have an increased likelihood of subclinical atherosclerosis (measured by CAC) compared to those with neither condition, and they also add to prediction of CAC over FRS, suggesting the importance of these factors in clinical assessment of CHD risk.