tbuckley/cabot-search huggingface dataset:
Folate Receptor–Positive Circulating Tumor Cell Detected by LT-PCR–Based Method as a Diagnostic Biomarker for Non–Small-Cell Lung Cancer
Authors: Xiaoxia Chen, Fei Zhou, Xuefei Li, Guohua Yang, Ling Zhang, Shengxiang Ren, Chao Zhao, Qinfang Deng, Wei Li, Guanghui Gao, Aiwu Li, Caicun Zhou
Journal: Journal of Thoracic Oncology (2015)
Abstract 15113: A Novel Purification Method for Cardiomyocytes and Endothelial Cells Derived From Human Pluripotent Stem Cells Using Microrna-responsive Messenger Rna
Authors: Kenji Miki, Seiya Takahashi, Shunsuke Funakoshi, Shinya Yamanaka, Hirohide Saito, Yoshinori Yoshida
Journal: Circulation (2014)

MicroRNAs (miRNAs) are small non-coding RNA that controls gene expression through translational regulation or mRNA cleavage. Recent progress in synthetic biology demonstrated that synthetic “RNA switches” enable to regulate gene expression depending on the intracellular environment. Here we show that synthetic mRNA technologies that modulate reporter protein expression by sensing miRNA activities enable purification of target cells in a safe and effective manner. To select cardiac-specific miRNA candidates, we performed miRNA microarray analyses using human induced pluripotent stem cell (hiPSC) line integrated MYH6 promoter driven EGFP cassette. We found 14 candidates, and synthesized individually these 14 miRNA-responsive OFF switch mRNAs (miR-switches) containing the sequence of blue fluorescent protein (TagBFP). We individually transfected them with EGFP mRNA into differentiated cells including cardiomyocytes derived from human pluripotent stem cell (hPSCs). We found that several cells transfected with miR-1- or miR-208-switches diminished TagBFP intensity, and sorting of these cells yielded high populations of troponin T-positive cardiomyocytes reproducibly and significantly (e.g., up to &gt;98% in five hPSC lines). In addition, we developed a non-sorting system for purification of cardiomyocytes using miR-switch that code apoptosis-inducible protein (up to &gt;97%). These cardiomyocytes purified by miR-switches were successfully engrafted in mouse heart and did not form tumor even when injected into testes of SCID mice. We next examined whether the system can be applied to purify endothelial cells, and confirmed that several miR-switches enabled high purification of CD31+ cells from differentiated derivatives of hPSCs (up to &gt;98%), which have the function of tube formation like HUVEC. Moreover, the use of multiple miRNA-responsive mRNAs enabled simultaneous and high purification of cardiomyocytes and endothelial cells from heterogeneous population derived from hPSCs induced by single differentiation method (up to &gt;98%). Our results suggest that miR-switch technology can be used for a variety of purposes including purification in any type of cells.
Abstract 3298: Effect of Enoxaparin vs Unfractionated Heparin in Diabetic Patients with ST-Elevation MI in the ExTRACT-TIMI 25 Trial
Authors: David A Morrow, Elliott M Antman, Carolyn H McCabe, Jie Qin, Frank Jiang, Eugene Braunwald
Journal: Circulation (2006)

Patients with diabetes mellitus (DM) are at significantly higher risk of complications after an acute myocardial infarction than are patients without DM. Thus, it is important to identify intervent...
The Unfinished Business of Developing Standards for End-of-Life Care: Leveraging Quality Improvement and Peer Review
Authors: Jeffrey T. Berger
Journal: The American Journal of Bioethics (2015)

"The Unfinished Business of Developing Standards for End-of-Life Care: Leveraging Quality Improvement and Peer Review." The American Journal of Bioethics, 15(8), pp. 50–51
Electromyographic Measurement of Fatigue in Rheumatoid Arthritis and Neuromuscular Disease
Authors: J. A. R. Lenman, J. L. Potter
Journal: Annals of the Rheumatic Diseases (1966)

Stiffness, particularly morning  is a characteristic symptom of active Rheumatoid Disease. An articular component of stiffness has been measured directly in a number of studies (Scott, 1960; Wright and Johns, 1961). Stiffness in muscle is less readily measured and has been given relatively little attention. Stiffness in both muscle and joint increase the load on actively contracting muscle, and can therefore be expected to hasten the onset of muscular fatigue. In an isometric contraction, however, the effect of joint stiffness would presumably be minimal, and the development of fatigue would reflect mainly the muscular component of stiffness. If the rate of development of fatigue could be measured objectively, this should provide information regarding the state of the muscles in rheumatoid disease, as well as in other disorders associated with stiffness or weakness. This report presents the results of a study of the changes in electrical activity of muscle occurring with fatigue in healthy individuals, patients with rheumatoid disease, and patients with neuromuscular disorders. The changes in the pattern of the myogram occurring with fatigue can be studied in records obtained with surface electrodes. It has been reported that the potentials increase in amplitude and diminish in frequency as fatigue develops (Cobb and Forbes, 1923). Quantitative information about these changes may be derived by graphic or electronic integration of the myogram. It has been shown that the mean voltage of the electromyogram is proportional to the tension of an isometric contraction (Lippold, 1952). The electrical activity
Abstract 19219: Differential Response of Murf-1 and Mafbx Expression in the Skeletal Muscle to Training Interventions in Heart Failure Patients
Authors: Stephan Gielen, Marcus Sandri, Volker Adams, Irina Kozarez, Robert Hoellriegel, Norman Mangner, Rainer P Hambrecht, Gerhard Schuler
Journal: Circulation (2010)

Introduction: Despite its prognostic implications the mechanisms of muscle wasting in chronic heart failure (CHF) are still incompletely understood. We have previously documented activation of the ...
Abstract 19113: Fractional Flow Reserve versus Angiography to Assess Progression of Atherosclerosis
Authors: Argyrios Ntalianis, Bernard De Bruyne, Catalina Trana, Fabio Mangiacapra, Olivier Muller, Aaron Peace, Michalis Hamilos, Jozef Bartunek, William Wijns, Emanuele Barbato
Journal: Circulation (2010)

Fractional flow reserve (FFR) is superior to quantitative coronary angiography (QCA) in assessing coronary stenosis severity. Whether FFR can also be used to evaluate atherosclerosis progression ha...
Mutations In XPO1, POT1, BIRC3 and FBXW7 collectively Predict Poor Outcome At Diagnosis In CLL and MBL Independent From The SF3B1 and NOTCH1 Status
Authors: Mathew J Rose-Zerilli, Jade Forster, Mathew Parry, Anton Parker, Anne Gardiner, Zadie Davies, Andrew J Steele, Helen Parker, Andrew Collins, Nick C.P. Cross, David Oscier, Jonathan C Strefford
Journal: Blood (2013)
Proton magnetic resonance spectroscopy investigation of hyperventilation in subjects with panic disorder and comparison subjects
Authors: S R Dager, W L Strauss, K I Marro, T L Richards, G D Metzger, A A Artru
Journal: American Journal of Psychiatry (1995)

The purpose of this study was to investigate differential effects of hyperventilation on brain lactate in patients with panic disorder and comparison subjects as a possible mechanism for explaining previous observations of an excess rise in brain lactate among panic disorder subjects during lactate infusion.Seven treatment-responsive patients with panic disorder and seven healthy comparison subjects were studied with proton magnetic resonance spectroscopy to measure brain lactate during controlled, voluntary hyperventilation over a period of 20 minutes. Hyperventilation was regulated with the use of capnometry to maintain end-tidal PCO2 at approximately 20 mm Hg during the period of hyperventilation. Blood lactate was measured prior to and at the end of hyperventilation.At baseline the two groups had similar brain lactate levels. Panic disorder subjects exhibited significantly greater rises in brain lactate than comparison subjects in response to the same level of hyperventilation. Blood lactate levels before and after 20 minutes of hyperventilation were not significantly different between groups.Controlled hyperventilation increases brain lactate and does so disproportionately in subjects with panic disorder. This increase in brain lactate may result from decreased cerebral blood flow due to hypocapnia, and individuals with panic disorder may have greater sensitivity to this regulatory mechanism.
Surveillance for the 2009 pandemic influenza A (H1N1) virus and seasonal influenza viruses - New Zealand, 2009.
Authors: Q. S. Huang, D. Bandaranayake, L. D. Lopez, R. Pirie, M. Peacey, R. Hall, J. Bocacao, B. Adlam, V. Hope, M. Croxson, I. Basu, S. Roberts, J. Lindeman, C. Gale, C. Mansell, S. Rooker, L. Olsen, T. Blackmore, L. Jennings, A. Werna, K. Barrett, D. Hunt, M. Jacobs, F. McGrath, G. Mackereth, J. S. O'Keefe
Journal: MMWR Morbidity and Mortality Weekly Report (2009)
Abstract 16777: Identification of Cardioprotective Factors Produced by Fetal Mesenchymal Stem Cells Through a Combined Transcriptomic and Proteomic Approach
Authors: Patrizia Danieli, Lennard Dekker, Lennaert Kleijn, Elisabetta Cervio, Rudol A de Boer, Theo Luider, Massimiliano Gnecchi
Journal: Circulation (2011)

Background: mesenchymal stem cells of fetal origin (F-MSC) can be isolated from the amniotic membrane of human placenta. We have data showing that F-MSC exert remarkable cardioprotective and proangiogenic effects through paracrine mechanisms. However, the complete nature and scope of the soluble mediators of cardioprotection have not been investigated yet. Methods: F-MSC were isolated from human term placenta. Human dermal fibroblasts were used as control population. Total RNA and proteins were extracted from cultured F-MSC (N=10) and fibroblasts (n=10) after 48 hrs of serum deprivation. Genome-wide expression profiling was performed on RNA samples with Illumina platform. Proteins extracted from both cell populations were analyzed by liquid chromatography-Fourier transform mass spectrometry (LC-FTMS). Results: whole-transcriptome gene expression analysis revealed that 647 genes are significantly over-expressed in F-MSC vs fibroblasts. Among these genes, 131 are up-regulated more than 2 fold in F-MSC. Computational analysis led to the identification of 32 genes encoding for secreted factors. Among them, the known cardioprotective factors Midkine (MDK) and SPARC are highly up-regulated in F-MSC vs fibroblasts. qPCR confirmed that MDK (+8 fold, p&lt;0.01) and SPARC (+5.8, p&lt;0.01) are up-regulated in F-MSC. Intriguingly, the role of the majority of the identified secreted factors in heart repair is still unknown. LC-FTMS proteomic analysis identified 266 proteins significantly up-regulated in F-MSC vs fibroblasts, including 22 secreted proteins. Some of these candidates may have a potential beneficial role in heart repair. In particular, Pentraxin-3 (PTX3) and Galectin-1 (GAL1) emerged as putative mediators of the cardioprotective effects exerted by F-MSC. Conclusion: using a combined transcriptomic and proteomic approach we identified known and novel putative cardioprotective factors produced by F-MSC. In particular, MDK, SPARC, PTX3 and GAL1 seem strong candidates. Further studies will help to fully dissect their role in myocardial repair.
Abstract 20969: Higher Implant Norepinephrine Transporter Levels in the Myocardium are Associated with Myocardial Recovery during Left Ventricular Assist Device Support
Authors: Robert S George, Paul Facer, Magdi H Yacoub, Praveen Anand, Emma J Birks
Journal: Circulation (2010)

Cardiac norepinephrine transporter (NET) uptake sites are markedly impaired in heart failure (HF) patients resulting in intracardiac abundance of norepinephrine (NE) which in turn overactivates the cardiac sympathetic system, a hallmark in the progression of HF. Left ventricular assist devices (LVADs) improve NET activity and reduce NE levels. The bearing of NET, however, in determining which LVAD patients favourably recover has not been studied. The aims of this study were to quantify NET using immunostaining in the myocardium of HF patients acquired during LVAD implantation and to correlate the NET expression with subsequent recovery.   Methods:  Left ventricular (LV) apical cores were obtained from patients with end stage HF (cardiac index 1.49±0.33 l/min/m 2 ) at the time of HeartMateII implantation. Tissue was snap frozen at -80°C. After fixation, sections were incubated overnight at 4°C with primary rabbit NET antibody (ATLAS Antibodies) at 1:100 concentrations. Secondary antibody was applied and avidin biotin peroxidase complex was used for labelling. Perioxidase was then developed using nickel enhanced diaminobenzidine to produce different colorimetric layers. Images were captured digitally and analysed by two independent observers using analySIS software (Olympus version 5.0). NET immunoreactivity was calculated as the average percentage area covered from randomly selected 5 different regions for each patient.   Results:  16 patients (14 males) were studied. 11 had recovered and had their LVAD explanted. The percentage NET immunoreactive nerve fibers per area were significantly higher in the recovered patients at the time of implantation (1.04±0.30% vs 0.65±0.43%, P=0.05). There was a moderate correlation between NET immunoreactive nerve fibers and myocardial recovery (R=0.512, P=0.035). Intraclass correlation coefficient between both observers revealed a moderate reliability of 45.8% (R= 0.464, P=0.028).   Conclusion:  Patients recovered with LVAD support had a higher percentage of NET immunoreactive nerve fibers per total area in the LV apex at the time of implantation as compared to non recovered patients. Immunostaining evaluation of NET immunoreactive nerve fibers at implantation correlated with subsequent myocardial recovery.
Acute Myeloid Leukemia
Authors: Margaret R. O'Donnell, Camille N. Abboud, Jessica Altman, Frederick R. Appelbaum, Steven E. Coutre, Lloyd E. Damon, James M. Foran, Salil Goorha, Lori J. Maness, Guido Marcucci, Peter Maslak, Michael M. Millenson, Joseph O. Moore, Farhad Ravandi, Paul J. Shami, B. Douglas Smith, Richard M. Stone, Stephen A. Strickland, Martin S. Tallman, Eunice S. Wang
Journal: Journal of the National Comprehensive Cancer Network (2011)

In 2010, approximately 12,330 people were diagnosed with and 8950 died of acute myeloid leukemia (AML). 1 As the population ages, the incidence of AML, along with myelodysplasia, seems to be rising.Equally disturbing is the increasing incidence of treatment-related myelodysplasia and leukemia in survivors of childhood tumors and young adulthood, such as Hodgkin disease, sarcomas, breast and testicular cancers, and lymphomas.Ionizing radiation and occupational exposure to benzene and petrochemicals are also associated with AML. 2 The NCCN AML Panel convenes annually to update guidelines for the diagnosis and treatment of AML in adults.
Combination of Tmprss6-ASO and the Iron Chelator Deferiprone Improves Erythropoiesis and Reduces Iron Overload in a Mouse Model of Beta-Thalassemia
Authors: Carla Casu, Mariam Aghajan, Rea Oikonomidau, Shuling Guo, Brett P. Monia, Stefano Rivella
Journal: Blood (2014)
Abstract 13001: Ventricular Assist Devices Do Not Treat Ventricular Arrhythmias: Significant Morbidity Persists After Implantation
Authors: Hannah Raasch, Brian Jensen, Brett Sheridan, Amanda Bowen, Anil Gehi, Eugene H Chung, John P Mounsey, Patricia Chang, Jason N Katz
Journal: Circulation (2011)

INTRODUCTION. Ventricular assist devices (VADs) have emerged as pivotal treatment options for patients with advanced heart failure. Despite robust left ventricular unloading, the right ventricle re...
Abstract 182: Risk of Cardiac Arrest by Location Type to Guide Future AED Placement in a Large Urban Center
Authors: Steven C Brooks, Jonathan Hsu, Sabrina K Tang, Roshanthakumar Jeyakumar, Timothy C Chan
Journal: Circulation (2011)

Introduction: Public access defibrillation (PAD) programs can improve survival from out-of-hospital cardiac arrest (OHCA). We aimed to identify high risk public locations which should be prioritized for automated external defibrillator (AED) placement. Objectives: 1) To determine the average annual incidence of cardiac arrest by location type within the study area, 2) To describe current registered PAD deployment by location type. Methods: An observational cohort study using the Resuscitation Outcomes Consortium Epistry database. We included all public location, non-traumatic, EMS-treated OHCAs from January 1, 2006 to June 30, 2010 in the City of Toronto. The total site counts in each location category were derived from a city planning database and used as the denominator in calculating average annual per site cardiac arrest incidence. AED locations were obtained from an EMS registry. Two investigators independently categorized each OHCA and AED location. Disagreements were resolved by consensus. Results: There were 1297 OHCAs, of which 571 occurred on streets and could not be associated with a specific location type, leaving 726 eligible cases. The average age was 58±17.5 years, 82.7% were male and 13.9% survived to hospital discharge. Of all included patients, 44.9% had bystander CPR attempted and 9.5% had a bystander apply a defibrillator. Table 1 contains data from the top 15 location types ranked on average annual OHCAs per individual site. There were 1616 AED covered locations in the City of Toronto. 57.3% of the registered AED sites were elementary and secondary schools, 16.0% were post secondary schools, 7.8% were offices and 4% were community or recreation centres. Conclusions: We have identified location types with highest risk for cardiac arrest. Most registered AEDs have been placed at Elementary and Secondary Schools which are relatively low risk for OHCA. Our data can guide future AED deployment to increase availability for use in public location OHCA.
Abstract 19348: The Impact of Hypothyroidism on Platelet-Fibrin Clot Characteristics in Patients Undergoing Cardiac Catheterization
Authors: Anand Singla, Kevin Bliden, Udaya Tantry, Young-Hoon Jeong, Paul A Gurbel
Journal: Circulation (2012)

Hypothyroidism and accompanying hypercholesterolemia are associated with accelerated atherosclerosis. Hypothyroidism has been associated with inﬂammation, endothelial dysfunction, and platelet function abnormalities. However, impact of hypothyroidism on the physical characteristics of the platelet-fibrin clot is unknown. We sought to determine the impact of hypothyroidism on platelet-fibrin clot characteristics in patients undergoing cardiac catheterization. Methods: Patients on 325 mg daily aspirin with (n=41) and without (n=286) a history of hypothyroidism undergoing cardiac catheterization were studied. Hypothyroidism was defined as treatment with thyroid hormone replacement. Blood samples were collected immediately prior to coronary angiography to determine clotting characteristics by thrombelastography (TEG). Urinary 11-dehydrothromboxane B 2 was performed by immunoassay method. Results: Baseline demographics and cardiac risk factors were similar between groups. Thyroid stimulating hormone levels were within the normal range with no significant difference between groups. Patients with a history of hypothyroidism had significantly higher thrombin-induced platelet fibrin clot strength(67.9±5.3 mm vs.66.1±5.8 mm, p=0.04) , faster time to initial thrombin formation (6.9±1.9 vs. 7.8±2.1, p=0.02) , and higher clotting index(0.7±2.4 vs. -0.4±2.0, p=0.005). There were no differences in urinary 11-dehydrothromboxane B 2 between groups. Conclusion: This is the first report that a history of hypothyroidism is associated with altered physical characteristics of the platelet-fibrin clot; an increase in the tensile strength of the clot. Further studies are needed to confirm this association and its relation to ischemic outcomes.
Abstract P325: Predictors of High Pulse Pressure and Change in Pulse Pressure with Age in the Atherosclerosis Risk in Communities (ARIC)Study
Authors: Kenneth R Butler, Deborah S Minor, Alan D Penman, Thomas H Mosley
Journal: Circulation (2013)

Introduction Pulse pressure (PP), an indirect measure of arterial stiffness, is a strong predictor of CVD risk. Determinants of PP and change in PP with age are not well described. Methods In a population-based cohort of 10,854 white and African-American men and women 44 - 66 years of age (mean 53.6 years) not taking anti-hypertensive therapy, SBP and DBP were measured at 4 visits 3 years apart (average 3.4 visits per person). At each visit three readings were taken using a random-zero sphygmomanometer and the average of the last two values computed. Results In a random effects linear model the average annual change in PP was 1.09 mm Hg (SE 0.02, P &lt;.0001), after accounting for baseline age differences. Race, sex, diabetes, smoking, drinking, and BMI were statistically significant predictors of average PP at any time point, but total and HDL cholesterol were not (Table). Diabetes, the most influential predictor, increased the average PP at any time point by 4.86 mm Hg (SE 0.41). Current (but not former) drinking was the only risk factor associated with a decrease in the average PP at any time point, by 1.05 mm Hg (SE 0.23). In addition, diabetes (women only) and BMI (women only) were associated with an increase, and current drinking with a decrease, in the average annual rate of change in PP. Conclusion In this middle-aged cohort, diabetes, BMI, and current drinking are important determinants of both the absolute level of PP and the annual rate of change in PP with age.
Abstract 16750: Phosphodiesterase 4B in the L-Type Calcium Channel Complex Regulates Cardiac Calcium Current and Protects Against Ventricular Arrhythmias
Authors: Jerome Leroy, Delphine Mika, Wito Richter, Liliana R Castro, Aniella Abi-Gerges, Moses Xie, Colleen Scheitrum, Florence Lefebvre, Julia Schittl, William A Catterall, Flavien Charpentier, Marco Conti, Rodolphe Fischmeister, Gregoire Vandecasteele
Journal: Circulation (2010)

Beta-adrenergic receptors (β-AR) enhance cardiac contractility by increasing cAMP levels and activating PKA. PKA increases Ca2+-induced Ca2+ release (CICR) by phosphorylating L-type Ca2+ channels (...
Abstract 12704: Activation of the Valosin-Containing Protein by H11 Kinase/Hsp22 Promotes Cardiac Cell Survival
Authors: Paulo Lizano, Huacheng Dai, Xiangzhen Sui, Christophe Depre
Journal: Circulation (2010)

The small heat shock protein H11 kinase/Hsp22 (Hsp22) stimulates cardiac cell survival through activation of the Akt pathway and expression of the inducible nitric oxide (NO) synthase (iNOS), resul...