tbuckley/cabot-search huggingface dataset:
ON THE IMPORTANCE OF MALARIA AS A CAUSE OF FALSE POSITIVE SEROLOGIC REACTIONS
Authors: T. R. Dawber
Journal: Annals of Internal Medicine (1943)

Article1 October 1943ON THE IMPORTANCE OF MALARIA AS A CAUSE OF FALSE POSITIVE SEROLOGIC REACTIONST. R. DAWBER, M.D.T. R. DAWBER, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-19-4-651 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe evaluation of positive serologic tests for syphilis is a problem which every physician must face. A diagnosis of syphilis is a serious matter, and its implications may be far reaching. Failure to start treatment in a known syphilitic as early as possible may well be grounds for malpractice. Equally serious and sometimes disastrous is a diagnosis of syphilis in some one who does not have the disease. From a public health point of view this may not be of much concern since in the general population of healthy individuals diagnostic errors due to false positive serologic reactions occur in...Bibliography1. MOOREEAGLEMOHR JEHCF: Biologic false positive serologic tests for syphilis. A suggested method of approach to their clinical study, Jr. Am. Med. Assoc., 1940, cxv, 1602. CrossrefGoogle Scholar2. MOHRMOOREEAGLE CFJEH: Biologic false positive serologic reactions in tests for syphilis. Occurrence with organic diseases other than syphilis, Arch. Int. Med., 1941, lxviii, 1161. CrossrefGoogle Scholar3. MOHRMOOREEAGLE CFJEH: Biologic false positive serologic reactions in tests for syphilis. Occurrence in normal persons, Arch. Int. Med., 1941, lxviii, 898. CrossrefGoogle Scholar4. EAGLE H: On the specificity of serologic tests for syphilis as determined by 40,545 tests in a college student population, Am. Jr. Syph., Gonor., and Ven. Dis., 1941, xxv, 7. Google Scholar5. KITCHENWEBBKUPPER SFELWH: The influence of malarial infections on the Wassermann and Kahn reactions, Jr. Am. Med. Assoc., 1939, cxii, 1443. CrossrefGoogle Scholar6. HAZENSENEARPARRANSANFORDSIMPSONVONDERLEHR HHFETAHWMRA: Serologic evidence of syphilis in malarial patients, Arch. Dermat. and Syph., 1938, xxxvii, 431; abstr. Jr. Am. Med. Assoc., 1938, cx, 1629. CrossrefGoogle Scholar7. WILSONLEVIN RAL: Observations on effect of malaria on Wassermann reaction, Am. Jr. Med. Sci., 1936, cxci, 696. CrossrefGoogle Scholar8. NAGELLLANGHANS HJ: Impf. Malaria und Wassermannsche Reaktion, Med. Klin., 1928, xxiv, 368. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: T. R. DAWBER, M.D.Affiliations: U.S.P.H.S. Stapleton, S. I., N. Y.*Received for publication May 19, 1942. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited bySerological tests for syphilis in treated Plasmodium falciparum malaria 1 October 1943Volume 19, Issue 4 Page: 651-655 Keywords Malaria Prevention, policy, and public health Syphilis ePublished: 1 December 2008 Issue Published: 1 October 1943 PDF downloadLoading ...
Abstract 3987: Evaluation of anti–malarial compounds as inhibitors of ezrin in osteosarcoma cells
Authors: Haydar Celik, Abraham Kallarakal, Daisy Colon-Lopez, Jeffrey Toretsky, Jurgen Bosch, Aykut Uren
Journal: Cancer Research (2014)

Abstract Ezrin is a member of the ERM (Ezrin, Radixin, Moesin) family of proteins that functions as a cross-linker between the plasma membrane and actin cytoskeleton, thus allowing the cell to regulate cytoskeletal dynamics in response to external stimuli. Ezrin plays important roles in cell motility, membrane trafficking, morphogenesis, adhesion, survival and apoptosis. Ezrin also participates in crucial signal transduction pathways. Overexpression of ezrin contributes to the aggressive cancer behavior in various tumor types especially by enhancing the metastatic phenotype. Thus, ezrin represents a promising molecular target for cancer therapy. In our earlier studies, we have established that NSC305787 can directly bind to ezrin and inhibit its activity, which results in reduced metastasis. Since NSC305787 shares significant structural similarities with some anti-malarial drugs, we tested 400 anti-malarial compounds including 200 drug-like compounds and 200 probe-like compounds for their potential to directly bind to ezrin protein. We used surface plasmon resonance technology (Biacore) to screen direct binding. In this initial screen, 12 compounds demonstrated binding to ezrin over background values and selected as primary hits. Detailed SPR analysis of primary hits resulted in 7 compounds with comparable KD values to that obtained with our lead compound NSC305787. Primary hits were also evaluated for inhibition of the cell motility of murine osteosarcoma (OS) cells K7M2 and K12 that express high and low levels of ezrin, respectively. Cell motility and chemotaxis assays were conducted in parallel with viability assays to rule out general toxicity. Selected anti-malarial compounds were effective in inhibiting the motility of OS cells with high ezrin compared to cells with low ezrin. In conclusion, several anti-malarial drugs may have new indications in cancer therapy by inhibiting ezrin. Citation Format: Haydar Celik, Abraham Kallarakal, Daisy Colon-Lopez, Jeffrey Toretsky, Jurgen Bosch, Aykut Uren. Evaluation of anti–malarial compounds as inhibitors of ezrin in osteosarcoma cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3987. doi:10.1158/1538-7445.AM2014-3987
Abstract 16790: Anti-Arrhythmic Properties of a Novel Cardiac Late Na+ Current Inhibitor, GS-6615, in a Mouse Model of Long QT Type 3
Authors: Sridharan Rajamani, Nesrine El-Bizri, Gongxin Liu, Luiz Belardinelli
Journal: Circulation (2014)

Background: Congenital Long QT syndrome type 3 (LQT3) is caused by mutations in the gene SCN5A resulting in enhanced late Na+ current (INa). Enhanced late INa leads to early-afterdepolarizations (EADs) and triggered activity (TA), spatiotemporal differences in repolarization time which promote reentrant electrical activity. This study was designed to investigate whether selective inhibition of late INa by GS-6615 (dihydrobenzoxazepinone) could effectively suppress ventricular arrhythmias in a transgenic mouse model of LQT3. Methods: The effects of GS-6615 on late INa and action potential duration (APD) in ventricular myocytes isolated from a transgenic mouse model of LQT3 (N1325S) were determined using whole-cell patch-clamp technique. Results: Compared to a transgenic WT mouse, the amplitude of late INa, APD to 50 and 90% repolarization (APD50 and APD90, respectively) and beat-to-beat variability of APD (BVR, Figure 1a and e) were significantly increased in N1325S ventricular myocytes. In addition, spont...
TRITIATED THYMIDINE LABELLING OF NORMAL HUMAN EPIDERMAL CELL NUCLEI.
Authors: J. M. LACHAPELLE, T. GILLMAN
Journal: British Journal of Dermatology (1969)

SUMMARY.— Only 2 methods for the incorporation of radioactive tracers into skin i.e. the local in vivo method and the in vitro method can be seriously considered for routine clinical use in man. Both methods-and especially the in vitro method as modified by us—are described in detail. The patterns of epidermal labelling and the labelling indices seen in the same skin fields, from the same 6 healthy human male volunteers, with the in vivo and in vitro techniques are directly compared. These two methods were found to yield very similar results. Thus, the average percentage of labelled basal cell nuclei was found to be 2·2% (range 1·9%, to 2·6%) with the local in vivo method and 2·3% (range 2·0% to 2·6%) with the in vitro method. The average percentage of labelled epidermal cell nuclei was found to be 2·1% (1·7% to 2·5%) with the in vivo method and 2·2% (range 1·4% to 3·0%) with the in vitro method for the same skin field. It is concluded that the local in vivo labelling method will probably provide invaluable data on cell and metabolite turnover rates, and its use should perhaps be limited to such studies. For all other purposes, however, the in vitro method, here detailed, seems to be preferable for routine clinical studies because of its safety, simplicity and reproducibility.
Abstract 4002: Exploring the miRNA and proteomic contents of exosomes isolated from rhabdomyosarcoma tumor cells to understand their paracrine signaling
Authors: Sandra E. Ghayad, Farah Ghamloush, Raya Saab
Journal: Cancer Research (2014)

Abstract Rhabdomyosarcoma (RMS) is an aggressive childhood soft tissue tumor, with two distinct subtypes, alveolar (ARMS) and embryonal (ERMS) histologies. The alveolar subtype is characterized by a specific translocation PAX-FOXO, the protein product of which is thought to contribute to its aggressive and metastatic behaviour. Exosomes are small membranous vesicles (30-100 nm in diameter) secreted into body fluids by multiple cell types, including tumor cells. Tumor exosomes contain intact and functional protein and microRNA that can alter the cellular environment to favor tumor growth. We hypothesize that the PAX-FOXO fusion protein results in specific effects on exosome cargo and biology, contributing to the invasive and metastatic potential of ARMS cells. Thus proteomic and molecular profiling of their exosomes may increase our understanding of the role of exosomes in sarcoma progression and may lead to discovery of useful biomarkers. In the present study, we used miRNA array profiling to identify interesting exosomal miRNA expressed commonly in two different ARMS cell lines. We also used proteomic analysis and discovered differentially expressed ARMS exosomal proteins. Importantly, functional studies revealed that normal fibroblasts acquired invasion and migration ability through molecules transported in ARMS cell-derived exosomes. Our results indicate that ARMS-derived exosomes have unique gene expression signatures,miRNA and proteomics profiles which will help our understanding of sarcoma biogenesis and that could be used as biomarkers for early diagnosis of ARMS, and targets for therapeutic intervention. Citation Format: Sandra E. Ghayad, Farah Ghamloush, Raya Saab. Exploring the miRNA and proteomic contents of exosomes isolated from rhabdomyosarcoma tumor cells to understand their paracrine signaling. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4002. doi:10.1158/1538-7445.AM2014-4002
Building a Medical Vocabulary
Authors: Peggy C. Leonard
Journal: Annals of Internal Medicine (1985)

This text provides the reader with an effective tool for learning medical terminology. With the help of activities such as fill-in-the-blanks, crossword puzzles and multiple-choice questions, students can quickly learn to recognize and write thousands of terms after learning the meanings of just a few word parts. The book includes an introduction to A&P, pronunciations of each medical term following its introduction, mini-reviews after each section and comprehensive end-of-chapter reviews.
Avian Influenza A (H5N1) Infection in Humans
Authors: John H Beigel, Jeremy Farrar, Aye Maung Han, Frederick G Hayden, Randy Hyer, Menno D de Jong, Sorasak Lochindarat, Thi Kim Tien Nguyen, Tran Hien Nguyen, Tinh Hien Tran, Angus Nicoll, Sok Touch, Kwok-Yung Yuen
Journal: New England Journal of Medicine (2005)
VENTRICULAR TACHYCARDIA: A CASE REQUIRING MASSIVE AMOUNTS OF PROCAINE AMIDE (PRONESTYL) FOR REVERSION
Authors: L J EMBREE, S A LEVINE
Journal: Annals of Internal Medicine (1959)

Case Reports1 January 1959VENTRICULAR TACHYCARDIA: A CASE REQUIRING MASSIVE AMOUNTS OF PROCAINE AMIDE (PRONESTYL) FOR REVERSIONLARRY J. EMBREE, M.D., SAMUEL A. LEVINE, M.D., F.A.C.P.LARRY J. EMBREE, M.D.Search for more papers by this author, SAMUEL A. LEVINE, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-50-1-222 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe following case is being reported because of the huge and unprecedented amount of medications required to control successfully a desperate cardiac complication, i.e., paroxysmal ventricular tachycardia. It was fairly clear from the experience during one of the episodes in this case that the tachycardia of itself might prove fatal. This permitted us—and encouraged us—to use the massive amounts of medication that proved to be necessary to control a subsequent attack.CASE REPORTThis 46 year old married machinist was admitted to the Peter Bent Brigham Hospital for the second time on June 27, 1957. He had apparently been in...Bibliography1. ArmbrustLevine CASA: Paroxysmal ventricular tachycardia: a study of one hundred and seven cases, Circulation 1: 28, 1950. CrossrefMedlineGoogle Scholar2. HarveyLevine WPSA: Paroxysmal ventricular tachycardia due to emotion, J. A. M. A. 150: 479, 1952. CrossrefMedlineGoogle Scholar3. Salley SM: An unusual atropin effect on ventricular tachycardia, Am. J. M. Sc. 183: 456, 1932. CrossrefGoogle Scholar4. GoodmanGilman LSA: The pharmacological basis of therapeutics, 2nd Ed., 1955, The Macmillan Company, New York, pp. 724-727. Google Scholar5. PascaleBernsteinSchoolmanFoley LRLMHMEF: Intravenous procaine amide in the treatment of cardiac arrhythmias, Am. Heart J. 48: 110, 1954. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Boston, Massachusetts*Received for publication September 15, 1957.From the Medical Clinic of the Peter Bent Brigham Hospital, Boston, Massachusetts.Requests for reprints should be addressed to Samuel A. Levine, M.D., 270 Commonwealth Avenue, Boston 16, Massachusetts. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byClinical use of antiarrhythmic drugsGlucagon in procainamide-induced cardiac toxicityMassive Doses of Procainamide for Ventricular Tachyarrhythmias due to Myocardial InfarctionVentricular tachycardia of 70 days' duration with survivalIntractable ventricular tachycardiaPersistent ventricular tachycardiaThe management of arrhythmias in acute myocardial infarction 1 January 1959Volume 50, Issue 1Page: 222-231KeywordsArrhythmiaHospitalsTachycardiaVentricular tachycardia ePublished: 1 December 2008 Issue Published: 1 January 1959 PDF downloadLoading ...
Abstract 19273: Fibroblast-Derived TSP1, via CD47, Upregulates Connective Tissue Growth Factor to Promote Left Ventricular Fibrosis and Heart Failure
Authors: Maryam Sharifi-Sanjani, Kevin Beezhold, Hallie Cook, Jeffrey Baust, Jeffrey Isenberg
Journal: Circulation (2014)

Background: An estimated 5.5 million individuals in the United States have left ventricular heart failure (LV HF). Therapeutics, while relieving symptoms and extending life in some cases, cannot resolve this process. LV HF is characterized by excessive accumulation of the extracellular matrix. The causes of this process remain incompletely understood. Our team has recently described a widely expressed cell surface receptor CD47 that is activated by its high affinity ligand, thrombospondin-1 (TSP1) to promote LV HF. However, the role of TSP1-CD47 signaling in promoting fibrosis, in general, and in the setting of LV HF, in specific, is unknown. Methods: Wild type mice expressing CD47 and mutated mice lacking CD47 (CD47 null) were subjected to transverse aortic constriction (TAC) for 4 weeks followed by open chest pressure-volume loop analysis of cardiac hemodynamics. Signal transduction was confirmed in isolated left ventricles (LV) and normal human ventricular fibroblast cell culture systems. Results: In biopsy samples from failing human hearts and in pre-clinical model of TAC-driven LV HF, TSP1-CD47 signaling was altered. Post-TAC wild type (CD47+/+) mice developed cardiac fibrosis, associated cardiac stiffness and HF. In contrast, CD47 null mice subjected to TAC showed enhanced cardiac function and decreased fibrosis concordant with suppression of TSP1. In cardiac resident fibroblasts, but not myocytes, hypoxia rapidly induced TSP1 protein, while treating with 7N3, a TSP1-based peptide mimetic that selectively binds CD47, increased connective tissue growth factor (CTGF) protein. Further, treating cardiac fibroblast with 7N3 increased fibroblast collagen production. Finally, CD47 blocking antibody mitigated established TAC-driven LV fibrosis and HF. Conclusion: These data identify a proximate role for activated cardiac fibroblast CD47 in promoting LV fibrosis associated with HF and suggest possible therapeutic opportunity.
Abstract 16: Protective Effect of Nicorandil, a Mitochondrial ATP-Sensitive Potassium (mKATP) Channel Opener, on Mitochondrial Impairments Can Prolong Survival in Alpha-Beta-Crystallin R120G Transgenic Mice
Authors: Atsushi Sanbe, Tetsuro Marunouchi, Kouichi Tanonaka, Hideo Nishigori, Akito Tanoue
Journal: Circulation (2010)

Background: Transgenic (TG) mice with cardiac-specific overexpression of an arg120gly (R/G) missense mutation in alpha-beta-crystallin (CRYAB), display desmin-related cardiomyopathy (DRM), which is...
Gene Transfer of the Runx2 Transcription Factor Enhances Osteogenic Activity of Bone Marrow Stromal Cells in Vitro and in Vivo
Authors: Zhuoran Zhao, Ming Zhao, Guozhi Xiao, Renny T. Franceschi
Journal: Molecular Therapy (2005)

Marrow stromal cells (MSCs) have the potential to differentiate into multiple mesenchymal cell types. To harness the power of MSCs for bone regeneration, methods must be developed to direct their differentiation selectively to the osteoblast lineage. The objective of this study was to examine the feasibility of using ex vivo Runx2 gene transfer to enhance the osteogenic activity of MSCs. Primary MSCs isolated from C57BL6 mice were transduced with adenoviral vectors encoding beta-galactosidase or Runx2. Cells transduced with Ad-Runx2 expressed Runx2 protein and underwent osteoblast differentiation as measured by increases in alkaline phosphatase activity and mineralization. Time-course studies revealed that Runx2 protein was highest 1 day after transduction and declined below the limits of detection by 15 days. Osteoblast marker mRNA expression paralleled Runx2 levels. In contrast, Runx2-dependent mineralization persisted for the duration of the experiment. To assess in vivo osteogenic activity, Ad-Runx2-transduced and control MSCs were adsorbed to two different carrier scaffolds and subcutaneously implanted into C57BL6 mice. In both cases, MSCs expressing Runx2 formed substantially more bone than cells transduced with control virus. Taken together, these studies indicate that Runx2 gene transfer may be an effective route to enhance the osteogenic potential of MSCs.
Use of semantic workflows to enhance transparency and reproducibility in clinical omics
Authors: Christina L. Zheng, Varun Ratnakar, Yolanda Gil, Shannon K. McWeeney
Journal: Genome Medicine (2015)

Recent highly publicized cases of premature patient assignment into clinical trials, resulting from non-reproducible omics analyses, have prompted many to call for a more thorough examination of translational omics and highlighted the critical need for transparency and reproducibility to ensure patient safety. The use of workflow platforms such as Galaxy and Taverna have greatly enhanced the use, transparency and reproducibility of omics analysis pipelines in the research domain and would be an invaluable tool in a clinical setting. However, the use of these workflow platforms requires deep domain expertise that, particularly within the multi-disciplinary fields of translational and clinical omics, may not always be present in a clinical setting. This lack of domain expertise may put patient safety at risk and make these workflow platforms difficult to operationalize in a clinical setting. In contrast, semantic workflows are a different class of workflow platform where resultant workflow runs are transparent, reproducible, and semantically validated. Through semantic enforcement of all datasets, analyses and user-defined rules/constraints, users are guided through each workflow run, enhancing analytical validity and patient safety. To evaluate the effectiveness of semantic workflows within translational and clinical omics, we have implemented a clinical omics pipeline for annotating DNA sequence variants identified through next generation sequencing using the Workflow Instance Generation and Specialization (WINGS) semantic workflow platform. We found that the implementation and execution of our clinical omics pipeline in a semantic workflow helped us to meet the requirements for enhanced transparency, reproducibility and analytical validity recommended for clinical omics. We further found that many features of the WINGS platform were particularly primed to help support the critical needs of clinical omics analyses. This is the first implementation and execution of a clinical omics pipeline using semantic workflows. Evaluation of this implementation provides guidance for their use in both translational and clinical settings.
Failure to Rescue Metric in Lung Surgery
Authors: Thomas K. Varghese
Journal: JAMA Surgery (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal