tbuckley/cabot-search huggingface dataset:
Abstract #5559: Unsaturated glycoside of genistein induces severe microtubule depolymerization and mitotic arrest in ovarian cancer cells
Authors: Ahmed Ahmed, Izabela Fokt, Krystiana Krzysko, Bogdan Lesyng, Robert Bast, Waldemar Priebe
Journal: Cancer Research (2009)

Genistein is a naturally occurring flavonoid that is found in soy derived products. As genistein exhibits antiangiogenic and antiproliferative activities, the compound has potential as an anti-cancer agent. Although the exact anti-tumor mechanisms remain unknown, genistein can inhibit tyrosine kinases and topoisomerase II. Poor solubility has hindered development of genistein as a chemotherapeutic agent. To improve its solubility, a series of derivatives has been prepared by different laboratories, including sugar glycosides. A unique glycoside of genistein (G21) has been identified that inhibits proliferation of ovarian cancer cells. The effect of G21 resembled that of microtubule inhibiting drugs, which arrest the cell cycle in the G2/M phase. To examine the effect of G21 on microtubules, we optimized a method to measure microtubule mass using immunofluorescence and automated microsopy in microplates. Binding of an anti-alpha tubulin antibody was detected using a secondary anti-mouse antibody tagged with alexa-488. The intensity of the fluorescence signal varied directly with the microtubule mass. The effect of G21 was compared to two known agents that affect microtubule stability: paclitaxel and nocodazole. As expected, within one hour of treatment, paclitaxel (100 nM) produced a significant increase in microtubule mass while nocodazole (2 \#956;M) almost abolished the fluorescence signal compared to diluent treated cells (p   Citation Information:  In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr 5559.
Delayed Cardiac Tamponade After Radiofrequency Catheter Ablation of Atrial Fibrillation
Authors: Riccardo Cappato, Hugh Calkins, Shih-Ann Chen, Wyn Davies, Yoshito Iesaka, Jonathan Kalman, You-Ho Kim, George Klein, Andrea Natale, Douglas Packer, Cristian Ricci, Allan Skanes, Marco Ranucci
Journal: Journal of the American College of Cardiology (2011)
Abstract 16486: Non-Contrast T1-Mapping is a Novel Diagnostic Tool for Acute Myocarditis on Cardiovascular Magnetic Resonance Imaging
Authors: Vanessa M Ferreira, Stefan K Piechnik, Erica Dall'Armellina, Theodoros D Karamitsos, Jane M Francis, Robin P Choudhury, Attila Kardos, Matthias G Friedrich, Matthew D Robson, Stefan Neubauer
Journal: Circulation (2011)

Background: The accurate diagnosis of acute myocarditis on cardiovascular magnetic resonance imaging (CMR) often requires multiple modalities, including T2-weighted (T2w), early and late gadolinium...
Postnatal care also requires evaluation
Authors: R. Bell
Journal: BMJ (1996)

shows this adequately.The Edinburgh postnatal depression scale is a validated tool for screening for psychiatric morbidity, which is indicated by a score of ¢ 12 or 13.4 Presenting these data in the form of a mean and SD does little other than describe the range of emotional and mental states of the sample.To show the incidence of postnatal depression it would have been better to include the number or percentage of patients in each group who exceeded the threshold score and to analyse the significance of any difference between these.As the paper stands, we are left with the information that women randomised to fewer antenatal visits were more anxious, worried, and likely to have negative feeling about their babies than women randomised to traditional care, but we do not have sufficient information to determine whether more of these women are at risk of postnatal depression.
Telling parents all relevant details might reduce recruitment of children to trials
Authors: T. Stephenson, D. A Walker
Journal: BMJ (1996)

EDITOR,—Charlotte Williamson argues that patients in clinical trials have “the right to know all relevant details about the situation in which they find themselves.”1 If the investigators seeking consent can truly tell the patient that they do not know which treatment is better then there is not a problem. Often, however, such equipoise does not exist because the investigators suspect that one treatment is superior but a randomised controlled trial is still necessary. If the investigators share their suspicions …
Abstract 16280: Early Regional Neuroinflammatory Profile After Asphyxial Cardiac Arrest Vs. Ventricular Fibrillation Cardiac Arrest Model in Rats
Authors: Thomas Uray, Cameron Dezfulian, Abigail Palmer, Jason P Stezoski, Keri Janesko-Feldman, Patrick M Kochanek, Tomas Drabek
Journal: Circulation (2014)

Introduction: The post-resuscitative care for survivors of cardiac arrest (CA) is independent of the etiology of CA. However, significant differences in outcomes from asphyxial CA (ACA) vs. ventricular fibrillation CA (VFCA) exist. Hypothesis: We hypothesized that early systemic and regional neuroinflammatory cytokine response could differ between ACA vs. VFCA. Methods: Adult male rats were subjected to 10 min asphyxia or 10 min VFCA (n=8/group). Three hours after return of spontaneous circulation (ROSC), systemic (plasma) and regional brain cytokine profile was assessed in four selectively vulnerable brain regions: cerebellum (CEREB), cortex (CTX), hippocampus (HIP) and striatum (STRI) using 10-plex cytokine array (Luminex). Results: Asphyxia resulted in ACA with a no-flow time of ~ 6±1 min vs. 10 min in VFCA. Lactate levels were higher after VFCA vs. ACA (Table 1). There were no differences in plasma cytokines, or brain interleukin (IL)-4, IL-6 or GM-CSF. Globally, IFNg was higher in ACA vs. VFCA, while...
Google Search Trends and Skin Cancer
Authors: Romi Bloom, Kyle T. Amber, Shasa Hu, Robert Kirsner
Journal: JAMA Dermatology (2015)

ues decreased, MI values increased monotonically.We then analyzed how these measurements correlated after raw values were categorized.Although ITA and MI values place individuals into 1 of 6 skin types, these classification systems are currently unrelated, with no consensus about which MI values belong to which FST group. 4,5 We found that by placing participants with MI values of 750.0 or greater in FST VI, we observed a very strong correlation between these unrelated classification systems (Spearman ρ = 0.95; P < .001)(Figure ,B).Discussion | Determining skin type is necessary for understanding personal risk for sunburn and, by extension, skin cancer.Skin type is also important clinically because the cosmetic and medical industries have increased their use of laser applications in recent years. 6 Because questions about FST are used to assign skin type and determine laser-based treatment variables, participants were asked questions about FST, and 538 (96.8%) stated that the sun affected their skin in some way.Of the 390 black participants, 373 (95.6%) acknowledged that they were photosensitive (Table ).Only individuals who are not photosensitive are typically classified as FST VI, and our data confirm that most black participants should be classified as having an FST other than VI. 3 As a result, we defined the MI for FST VI to include only individuals with an MI of 750.0 or greater.Strong correlation between MI and ITA values (Figure) suggests that either of these methods can be used to assess skin pigmentation depending on the relevance of the measurement outcome of the intended study.Recognizing this strong correlation will allow research by health care professionals, biomedical scientists, and public health researchers to be more applicable and comprehensible across disciplines.
Gender identity in childhood and later sexual orientation: follow-up of 78 males
Authors: Richard Green
Journal: American Journal of Psychiatry (1985)

Two groups of males were evaluated on parameters of gender identity, initially in boyhood and later in adolescence or young adulthood. One group was composed of 66 clinically referred boys whose behaviors were consistent with the diagnosis of gender identity disorder of childhood. The other group consisted of 56 volunteers selected on the basis of demographic matching. Two-thirds of each group were reevaluated for sexual orientation; 30 of the 44 who previously had shown extensive cross-gender behavior and none of the 34 in the comparison group were bisexually or homosexually oriented.
Th17 inflammation and pulmonary hypertension
Authors: Aurélie Hautefort, Barbara Girerd, David Montani, Marc Humbert, Frédéric Perros
Journal: European Respiratory Journal (2014)

Background  :  Pulmonary arterial hypertension (PAH) is a severe disease resulting in right heart failure, in which inflammation and autoimmunity mechanisms may play a role.   Aim: To decipher the PAH fingerprint on the immune response orchestrated by dendritic and T cells (DC and LT).   Methods  :  We performed an immunophenotyping of monocyte-derived DC (MoDC) and circulating lymphocytes from PAH and control patients by flow cytometry, and analyzed the cytokine profile of both populations after stimulation and/or coculture. We tested the immunomudulatory effects of dexamethasone (Dex) on these parameters, and confirmed by an epigenetic approach, the PAH immune polarization in blood DNA.   Results:  Under similar differentiation/activation conditions, PAH MoDCs display similar profile of costimulatory molecules as compare to controls. However, PAH MoDCs expressed higher level of the LT activating molecules CD86 and CD40 after Dex pretreatment than controls. This was associated with an increased expression in p40 (IL12 and IL23 subunit) and a reduced migration toward CCL21. Both with and without Dex, PAH MoDC induced a higher activation/proliferation of CD4+ LT, associated to a reduced expression of IL4 (Th2 response) and higher expression of IL17 (Th17 response). In PAH venous blood, we found a specific depletion of CD4+ LT, possibly recruited in pulmonary vascular lesions. Purified PAH CD4+ LT expressed higher level of IL-17 after activation than controls. At last, there was a significant increase in the % of demethylated DNA at the IL17 promoter, in the PAH blood DNA as compared to controls.   Conclusions:  We highlighted a Th17 immune polarization in PAH patient; a polarization involved in several chronic inflammatory and autoimmune conditions.
Paclitaxel (T), ifosfamide (I), and carboplatin (Cb) (TICb) combination chemotherapy in advanced uterine and adnexal malignant mixed mullerian tumors (MMMTs)
Authors: C. Kosmas, N. Mylonakis, G. Tsakonas, G. Vorgias, N. Pantelis, P. Politis, N. Kalinoglou, N. Tsavaris, T. Akrivos, A. Karabelis
Journal: Journal of Clinical Oncology (2009)

5517 Background: Malignant mixed mullerian tumors (MMMTs) of the uterus and adnexa represent aggressive gynecologic malignancies with a high rate of locoregional and distant failure. For that reason we evaluated the TICb combination in patients with advanced MMMTs. Methods: Patients with advanced MMMTs [stages III-IV and relapses after surgery (Sx)±RT], WHO-PS 0–2, no prior chemotherapy, unimpaired hematopoietic/organ function were eligible. Chemotherapy was administered as follows; T: 175 mg/m 2 d1, I: 2.0 g/m 2 /d-d1+2, and Cb at a target AUC = 5 (according to creatinine clearance based on Calvert's formula) on d2 after I. G-CSF was administered from day 3–7. Results: Thirty-two patients with MMMTs of the uterus (n = 28), tubes (n = 2), or ovary (n = 2) have entered so far and all are evaluable for response and toxicity: median age = 61 (45–72), PS = 1 (0–1), stages; III = 18 (56%), IV = 14 (44%), histologies were; with homologous sarcoma component: 21, with heterologous component: 11. Prior treatment for locoregional disease included Sx: 23, Sx+RT: 9. Disease sites at diagnosis included: pelvic disease 12; pelvic/paraortic lymph nodes 14; peritoneal implants 16; liver 4; lung nodules 9; bone metastases 1; malignant pleural effusion 4. Responses were as follows: 21/32 (66%) evaluable patients responded, with 9 complete responses (CR) and 12 partial responses (PR), while 8 had stable disease (SD), and 3 developed progressive disease (PD). The median response duration was 8 mo (4–28), median time-to-progression (TTP) 12.5mo (4–26), while median overall survival (OS) has not been reached yet since most patients receive second-line therapy. Grade (Gr) 3/4 toxicities included: neutropenia 18/32 (56%)-with 11 developing Gr4 (≤7 days) and 19% of patients at least one episode of febrile neutropenia managed successfully by broad spectrum antibiotics, thrombocytopenia Gr3: 5/32 (16%) and Gr4 3/32 (9%), no Gr3 neuropathy, Gr1 CNS toxicity in 1, no renal toxicity, 15 Gr2 myalgias, and 4 Gr3 vomiting. Conclusions: In the present study, it appears that the TICb combination, yielded important activity with acceptable toxicity in females with advanced MMMTs. No significant financial relationships to disclose.
How common are myeloproliferative neoplasms? A systematic review and meta‐analysis
Authors: Glen J. Titmarsh, Andrew S. Duncombe, Mary Frances McMullin, Michael O'Rorke, Ruben Mesa, Frank De Vocht, Sarah Horan, Lin Fritschi, Mike Clarke, Lesley A. Anderson
Journal: American Journal of Hematology (2014)

Myeloproliferative neoplasms (MPNs) are a heterogeneous group of diseases including polycythemia vera (PV), essential thrombocythemia (ET), and primary(idiopathic) myelofibrosis (PMF). In this systematic review, we provide a comprehensive report on the incidence and prevalence of MPNs across the globe. Electronic databases (PubMed, EMBASE, MEDLINE, and Web of Science) were searched from their inception to August 2012 for articles reporting MPN incidence or prevalence rates. A random effects meta-analysis was undertaken to produce combined incidence rates for PV, ET, and PMF. Both heterogeneity and small study bias were assessed. Thirty-four studies were included. Reported annual incidence rates ranged from 0.01 to 2.61, 0.21 to 2.27, and 0.22 to 0.99 per 100,000 for PV, ET, and PMF, respectively. The combined annual incidence rates for PV, ET, and PMF were 0.84, 1.03, and 0.47 per 100,000. There was high heterogeneity across disease entities (I(2) 97.1-99.8%) and evidence of publication bias for ET and PMF (Egger test, P = 50.007 and P ≤ 0.001, respectively).The pooled incidence reflects the rarity of MPNs. The calculated pooled incidence rates do not reflect MPN incidence across the globe due to the high unexplained heterogeneity. Improved, widespread registration of MPNs would provide better information for global comparison of the incidence and prevalence of MPNs.
Insights into<i>Campylobacter jejuni</i>colonization of the mammalian intestinal tract using a novel mouse model of infection
Authors: Martin Stahl, Bruce A Vallance
Journal: Gut Microbes (2015)

A lack of relevant disease models for Campylobacter jejuni has long been an obstacle to research into this common enteric pathogen. We recently published that mice deficient in Single IgG Interleukin-1 related receptor (SIGIRR), a repressor of MyD88-dependent innate immune signaling, were highly susceptible to enteric infection by murine bacterial pathogens. Subsequently, we successfully employed these mice as an animal model for the human pathogen C. jejuni and gained substantial new insights into infection by this pathogen. The infected mice developed significant intestinal inflammation, primarily via TLR4 stimulation. Furthermore, the resulting gastroenteritis was dependent on C. jejuni pathogenesis as bacterial strains suffering mutations in key virulence factors were attenuated in causing disease. The ability to infect SIGIRR-deficient mice with C. jejuni sheds new light onto how these bacteria colonize the mucus layer of the intestinal tract, invade epithelial cells, and raises new prospects for studying the virulence strategies and pathogenesis of C. jejuni.
Patients with panic attacks and abnormal EEG results
Authors: M J Edlund, A C Swann, J Clothier
Journal: American Journal of Psychiatry (1987)

The authors describe a series of patients who presented with atypical panic attacks involving hostility, irritability, severe derealization, and social withdrawal. None had clear temporal lobe epilepsy but most had temporal EEG abnormalities. Although their response to treatment was unpredictable, some did well with carbamazepine or alprazolam.
Sex differences in clinical characteristics and outcomes after myocardial infarction: insights from the Valsartan in Acute Myocardial Infarction Trial (<scp>VALIANT</scp>)
Authors: Carolyn S. P. Lam, Margaret McEntegart, Brian Claggett, Jiankang Liu, Hicham Skali, Eldrin Lewis, Lars Køber, Jean Rouleau, Eric Velazquez, Rob Califf, John J. McMurray, Marc Pfeffer, Scott Solomon
Journal: European Journal of Heart Failure (2015)
Abstract 16435: Lifestyle Interventions Reduce Ten-Year Risk of Atherosclerotic Cardiovascular Disease as Predicted by the Pooled Cohort Equations
Authors: Alan Hinderliter, Patrick Smith, Andrew Sherwood, James Blumenthal
Journal: Circulation (2014)

Introduction: Lifestyle measures, such as adoption of the DASH diet, weight reduction, and exercise, are effective in lowering blood pressure. The degree to which these behaviors reduce the risk of cardiovascular events is not well-established. We examined the effect of dietary and weight management inventions used in the ENCORE study on the 10-year risk of atherosclerotic cardiovascular disease (ASCVD), as defined by the Pooled Cohort Equations. Methods: Participants included 129 overweight or obese men and women between the ages of 40 and 80 years who had blood pressure 130-160/80-99 mmHg and who were not treated with antihypertensive medications. Subjects were randomized to 16 weeks of DASH diet plus behavioral weight management (consisting of nutritional counseling and a behavioral weight management program)(DASH+WM), DASH diet alone (consisting of nutritional counseling without exercise or caloric restriction)(DASH), or usual care (UC). Results: The participants averaged 53.5±8.8 years of age; 68% were female, and 60% were white. One had diabetes and 11 (9%) were smokers. Systolic BP averaged 138±9 mmHg; total and HDL cholesterol averaged 203±36 mg/dl and 55±14 mg/dl, respectively. The average estimated 10-year risk of ASCVD for the entire cohort was 5.7%. The baseline characteristics of the subjects randomized to DASH+WM (n=46), DASH (n=39), and UC (n=44) were similar. Participants in the DASH+WM and DASH interventions had significant decreases in systolic blood pressure, and those in the DASH+WM group had a significant decrease in total cholesterol. Post-intervention estimated 10-year ASCVD risk [mean(SE)], adjusted for baseline values, was 4.4(0.2)% for DASH+WM, 5.0(0.3)% for DASH, and 5.7(0.2)% for UC (p&lt;.001 for DASH+WM vs UC; p&lt;.05 for DASH vs UC). The number of untreated subjects with an indication for consideration of statin therapy, as recommended in the 2013 ACC/AHA Guideline for the Treatment of Blood Cholesterol, fell from 11 to 6 in DASH+WM and from 12 to 10 in DASH, while remaining unchanged (11) in UC. Conclusions: In overweight or obese men and women with high BP, lifestyle interventions reduce the predicted 10-year risk of ASCVD and decrease the number of individuals in whom statin therapy is recommended.
Detection of bcr-abl Transcripts in Philadelphia Chromosome–Positive Acute Lymphoblastic Leukemia After Marrow Transplantation
Authors: Jerald Radich, Gene Gehly, Arthur Lee, Rachel Avery, Eileen Bryant, Scott Edmands, Ted Gooley, Peter Kessler, Judy Kirk, Paula Ladne, E.D. Thomas, Frederick R. Appelbaum
Journal: Blood (1997)
Abstract 3792: Influence of Gender on the Extent of Myocardial Viability After Primary Coronary Angioplasty
Authors: Emanuele Canali, Antonella Mattatelli, Daniele Berardi, Stefania Funaro, Mariapina Madonna, Leda Galiuto, Sabrina Barchetta, Luciano Agati
Journal: Circulation (2009)

Objectives: Few studies have been addressed about sex distinctions in the amount of jeopardized myocardium that is salvaged by mechanical reperfusion in patients with acute myocardial infarction (AMI). The aim of our study was to evaluate possible gender-related differences in tissue viability after ST elevation myocardial infarction (STEMI) by using myocardial contrast echocardiography (MCE).  Methods: We studied 110 consecutive patients who underwent successful primary coronary angioplasty within 6 h of onset of STEMI. Two-dimensional echocardiography (2DE) was performed within 24 hours of coronary recanalization and microvascular perfusion was assessed by real-time MCE on 7±2 days after AMI. Contrast defect length (CDL%) was measured to assess microvascular integrity on endocardial border. Myocardial viability index was obtained by summing perfused but dysfunctioning segments divided by all dysfunctional segments and expressed as percentage.  Results: Of the 110 patients received primary PCI, 28 patients were females (26%) and 82 (74%) were males. Women were significantly older and had a longer time to reperfusion (8.6 + 7.1 hours vs 4.5 + 4.2 hours in men, p=0.011). Contrast defect length was larger in men than women (20 + 7.2 % vs 11 + 7.0 %, respectively, p=0.009) suggesting the presence of a bigger microvascular damage. Myocardial viability index was higher in women than in men (56 + 37 % vs 33 + 22 %, respectively, p=0.016). Multiple linear regression analysis was used to assess the independent impact of gender on myocardial viability. After adjustment for other variables, female gender, younger age, anterior infarction, shorter time to reperfusion, small contrast defect length and the use of glycoprotein IIb/IIIa inhibitors were identified as independent factors of higher myocardial viability.  Conclusions: In successfully reperfused STEMI population, myocardial viability as detected by MCE is gender-dependent also after adjusting for other baseline and risk factors. A different response to antiaggregating drugs, a higher tolerance to hypoxic setting and the activation of estrogen receptors against intracellular calcium loading could probably be involved in this favorable women behavior.