tbuckley/cabot-search huggingface dataset:
Abstract 13269: Reduced Expression of scaRNAs Disrupts Spliceosome Function and Heart Development in Zebrafish and Infants with Tetralogy of Fallot
Authors: Douglas C Bittel, Prakash Patil, Tamayo Uechi, Nataliya Kibiryeva, Jennifer Marshall, Mike Artman, James E O’Brien, Naoya Kenmochi
Journal: Circulation (2014)

The splicing of messenger RNA plays a fundamental role in regulating vertebrate development and differentiation. Although it is well established that alternative splicing (AS) plays an important role in regulating mammalian heart development, a clear link between misregulated splicing and congenital heart defects has not been shown. We recently reported that more than 50% of genes associated with heart development had significant changes in splice forms in the right ventricle of infants with tetralogy of Fallot (TOF; 14M/7F; all less than 1 yr old). Moreover, there was a significant decrease (30-50%, p&lt;0.05) in the level of 12 scaRNAs. scaRNAs are members of the large family of noncoding small RNAs that are responsible for biochemical modification of specific nucleotides in spliceosomal and ribosomal RNAs. These 12 scaRNAs target two spliceosomal RNAs, U2 and U6. We used primary cells derived from the RV of infants with TOF to show a direct link between scaRNA levels and splice isoforms of several key genes regulating human heart development (e.g., GATA4, NOTCH2, DAAM1, DICER1, MBNL1 and 2). In addition, using available RNA-Seq data, we provide evidence that during zebrafish development, there are dynamic oscillations in scaRNAs and splice isoforms of genes that regulate heart development. We knocked down the expression of two scaRNAs; ACA35 (Scarna1) and U94 (Snord94), in zebrafish and saw a corresponding disruption of heart development. Importantly, there was an accompanying alteration in the ratios of splice isoforms of key cardiac regulatory genes. Based on these combined results, we propose that scaRNAs directly regulate the proficiency of the spliceosome by controlling spliceosomal RNA maturation. This in turn contributes to splice isoform dynamic equilibrium and ultimately heart development. These results are consistent with a failure of normal temporal and spatial splicing patterns during early embryonic development, leading to a breakdown in communication between the first and second heart fields, resulting in conotruncal misalignment and TOF. Our findings represent a new paradigm for understanding congenital cardiac malformations.
Abstract 3289: Biochemical Markers of Myocardial Collagen Network Remodeling Discriminate Between Systolic and Diastolic Heart Failure in Hypertensive Patients
Authors: Arantxa Gonzalez Miqueo, Begona Lopez, Ramon Querejeta, Elena Zubillaga, Tomas Echeverria, Susana Ravassa, Javier Diez
Journal: Circulation (2009)
Abstract 18053: Use of Cardiocerebral Resuscitation or AHA 2005 Guidelines by EMS Improves Survival from Out-of-Hospital Cardiac Arrest: A Systematic Review and Meta-Analysis
Authors: Marcus Salmen, Gordon Ewy, Comilla Sasson
Journal: Circulation (2011)

Background: The 2005 American Heart Association (AHA) Guidelines on CPR advise minimal interruptions in chest compressions during out-of-hospital cardiac arrest (OHCA). Growing evidence suggests that use of Cardiocerebral Resuscitation (CCR) - compressions without ventilations - may increase survival. To date, no systematic review has been conducted to assess the impact of implementing CCR or AHA 2005 guidelines. Objective: To determine if Emergency Medical Services (EMS) implementation of CCR or AHA 2005 protocols improved patient outcomes from OHCA. Methods: We conducted a systematic search of electronic databases (MEDLINE, EMBASE, CENTRAL) and consulted experts to identify all potential studies. A priori study inclusion criteria were: 1) used human OHCA subjects, 2) compared CCR or AHA 2005 guidelines to AHA 2000 guidelines, and 3) met AHA criteria for “Good” quality. Results: We identified 681 potentially relevant articles. The 12 studies meeting inclusion criteria are shown with their data in Table 1. All 3 studies using CCR demonstrated improved survival compared to use of AHA 2000 guidelines, as did 5 out of the 9 studies using AHA 2005 guidelines. Pooled data demonstrates that use of a CCR protocol has an Odds Ratio of 2.25 (95% Confidence Interval [CI]: 1.81-2.79) for survival to hospital discharge among all cardiac arrest patients. Use of AHA 2005 Guidelines increases all patient survival by an OR of 1.39 (95% CI: 1.10-1.75). Conclusion: Our systematic review and meta-analysis demonstrated improved survival from OHCA when EMS use CCR or AHA 2005 guidelines. Additional research is needed to further define the difference in outcomes between these two protocols.
Variation in Prostate Cancer Care
Authors: Khurshid R. Ghani, David C. Miller
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
849. CD Spectra of Compacted DNA Nanoparticles Demonstrate a Unique DNA Structure
Authors: Minli Sun, Hao Zhang, Baigen Zhang
Journal: Molecular Therapy (2004)

Object: To find a new method to purify the VEGF plasmids. Methods: Three kinds of magnetic nanoparticles(MNP)(DEAE-MNP,triplex-MNP,probe-MNP) were prepared by coprecipitation and condensation reaction. The VEGF plasmids were purified in the bacteria lysis by MNPs. The purified plasmids were detected by UV spectrophotometer, agrose gel electrophoresis and gene sequencing. Results: (1)The high purity VEGF plasmids could be gained by three kind of MNP, the DEAE-MNP had the same results with the chromatography. (2)No impurity and other nucleic acid were seen in the image of eletrophoresis. (3)The gene sequencing accorded to the literature report. Conclusion: The new style magnetic nanoparticles offer a new way to purify the biological matter in the mixture.
Conformable Electronics: Tattoo Conductive Polymer Nanosheets for Skin‐Contact Applications (Adv. Healthcare Mater. 7/2015)
Authors: Alessandra Zucca, Christian Cipriani, None Sudha, Sergio Tarantino, Davide Ricci, Virgilio Mattoli, Francesco Greco
Journal: Advanced Healthcare Materials (2015)

Conductive tattoo nanosheets are fabricated on top of decal transfer paper and transferred onto skin or other target surfaces as temporary transfer tattoos. Tattoo nanosheets, reported by V. Mattoli, F. Greco, and co-workers on page 983, are envisioned as unperceivable human-device interfaces because of conformal adhesion to skin. They are tested as dry electrodes for surface electromyography (sEMG) which permits the control of a robotic hand.
1022. Ionizing Radiation-Induced Adenovirus Infection Is Mediated by Dynamin 2
Authors: Jiong Yang, Eyad Abu-Isa, Theodore S. Lawrence, Ming Zhang
Journal: Molecular Therapy (2004)

Specificity of viral targeting into intrahepatic tumors remains critical for adenovirus gene therapy in liver cancer. We previously demonstrated that regional ionizing radiation treatment increases adenovirus uptake and transgene expression in cells and colon cancer xenografts. In this study, we tested whether radiation induces viral uptake through virus-cell membrane interaction. We found that radiation at 8 Gy induced adenoviral gene transfer in rat hepatocytes (WB) and human colon carcinoma cells (LoVo) as measured by the number of positive cells for transgene expression. This induction (24.4- and 6.5-fold, respectively) was diminished by about 50% by pre-incubation with antibody for Dynamin 2 but not for Coxackie Adenovirus receptor (CAR) or for integrin av. This inhibition appeared to result from a lesser induction of viral uptake in anti-Dynamin 2-treated cells in response to irradiation. Radiation treatment increased Dynamin 2 expression in both cells detected by immunohistochemistry staining and western blotting, and stimulated mRNA levels for Dynamin 2 in WB (1.5-fold) and LoVo (2.0-fold) cells. Inhibition of gene expression by transfecting cells using siRNA specific for Dynamin 2 significantly decreased mRNA levels in both cell types (~50%) and abolished the induction of Dynamin 2 expression mediated by irradiation. Consistently, adenoviral gene transfer in transfected cells was significantly lowered (~ 1/3). Radiation induction of viral gene transfer in WB and Lovo cells (24.4- and 6.5-fold, respectively) was significantly reduced in siRNA-transfected cells (15.5-fold and 2.7-fold, respectively). These data suggest that ionizing radiation induce adenovirus gene transfer in cells by regulating viral uptake through interaction with cellular Dynamin 2 and should provide insight into improving adenovirus targeting in tumors.
A novel, high-throughput assay for detection of ABL T315I mutations
Authors: Michael C. Heinrich, Jason R. Morich, Emmanuel J. Beillard, Courtney Fuller, Chad D. Galderisi, Brian J. Druker
Journal: Clinical Cancer Research (2006)

B68  Mutations in the kinase domain of BCR-ABL result in impaired drug binding and are the leading cause of acquired resistance to the tyrosine kinase inhibitor imatinib (Gleevec®). While imatinib is a highly effective therapy in all stages of chronic myelogenous leukemia (CML), relapse after an initial response is common in patients with advanced disease. The T315I point mutation is one of the most common imatinib-resistant mutations and patients with this mutation are also resistant to two second generation tyrosine kinase inhibitors, dasatinib and nilotinib. Thus, appropriate detection of this mutation is essential to optimal management of patients with imatinib resistance and may be useful for clinical trials of agents that target patients with the T315I mutation. Current methods for mutation detection, such as direct DNA sequencing, are not sensitive enough for detection of point mutations at low levels of BCR-ABL transcript. Conversely, ultrasensitive detection methods such as allele specific PCR (AS-PCR) may be too sensitive and can display a propensity for false positives. In addition the clinical relevance/significance of mutation detection at ultra sensitive levels (
Nutritional hyperhomocysteinaemia
Authors: I V Mohan, G Stansby
Journal: BMJ (1999)

A raised concentration of serum homocysteine is increasingly being implicated as a risk factor for clinical vascular disease. It is known to be injurious to the vascular endothelium in animals,1 and many reports have now confirmed the presence of raised homocysteine concentrations in patients with peripheral vascular disease, myocardial infarction, stroke, and venous thromboembolism. Indeed, moderate and intermediate increases in homocysteine concentration have been found in up to 40% of patients with vascular disease2 and in up to 35% of patients with venous thromboembolism.3 The obvious question is: is this cause or effect? And, if it is causal, should we be screening for and treating hyperhomocysteinaemia?

All circulating homocysteine is derived from methionine in the diet, and it is removed by either remethylation to methionine or conversion to cysteine via a transsulphuration pathway. Classic homocystinuria, a rare autosomal recessive condition, of which premature vascular disease and thrombosis are major features, is the result of a deficiency of …
Pharmacology and Toxicology of certain Organic Phosphorus Insecticides. Report from the Committee on Pesticides to the Council on Pharmacy and Chemistry.
Authors: S. A. Rohwer, H. L. Haller, K. Dubois, D. Grob, H. K. Abrams, D. O. Hamblin, J. F. Marchand, A. J. Ehman, A. Hartzell, J. C. Ward
Journal: JAMA (1950)
Abstract 879: Utility of Heart Rate Variability to Identify High Risk Subset for Cardiovascular Disease Among Patient With Chronic Kidney Disease
Authors: Masanori Shiba, Masato Nakamura, Nobuhiko Joki, Kazuyuki Ishi, Masaki Hori, Makoto Utsunomiya, Joe Aikawa
Journal: Circulation (2009)

Background: Chronic kidney disease (CKD) is well known as a risk factor for cardiovascular disease. However, relationship between those diseases is still unclear. Sympathetic nerve hyperactivity in...
Insulin Dosing in Pediatric Diabetic Ketoacidosis
Authors: David Carmody, Rochelle N. Naylor, Louis H. Philipson
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 1954: Is In-Stent Late Loss Valid to Predict the Risk of Binary Angiographic Restenosis and Target Revascularization in the Era of Drug-Eluting Stents?
Authors: Raul Moreno, Cristina Fernandez, Luis Calvo, Angel Sanchez-Recalde, Guillermo Galeote, Fernando Alfonso, Rosana Hernandez, Camino Banuelos, Rosa Sanchez-Aquino, Jose L Lopez-Sendon, Carlos Macaya
Journal: Circulation (2006)

In-stent late loss (ISLL) after drug-eluting stent (DES) implantation does not follow a normal distribution. Because of that, concerns exist about the degree of association between ISLL and the rat...
Abstract 13287: Dendritic Cells Play a Critical Role in the Initiation of Atherosclerosis
Authors: Mengyao Jin, Peng Liu
Journal: Circulation (2014)

Introduction: Dendritic cells (DCs) that are known as professional antigen-presenting cells have been found to pre-locate in non-inflammatory arterial wall and increasingly accumulate during atherosclerosis progression. Previous findings suggested that residential DCs in the intima are responsible for capturing modified lipids and forming foam cells during the initiation of atherosclerosis. Hypothesis: DC accumulation and enhanced DC-T cell interaction play a critical role in the initiation of atherosclerosis. Methods: We measured plaque formation, vascular DC accumulation and antigen-specific T cell proliferation mediated by isolated aortic cells in ApoE-/- mice, as well as DTR-CD11c/ApoE-/- or DTR-CD11b/ApoE-/- mice for conditional depletion of DCs or macrophages, respectively. A brief high-fat diet for 10 days was used as a model of initial atherosclerosis. Results: In addition to increased intimal DC accumulation and plaque formation in aortic roots, 10 days of HFD induced T cell infiltration in ApoE-/- mice, compared to those without HFD as the control. Isolated aortic cells from mice with 10-day HFD showed stronger capability in inducing antigen-specific T cell proliferation, compare to the control (HFD: 3.14±0.71%; no HFD: 1.56±0.36%; p=0.022). Single diphtheria toxin (DT) injection at day 1 yielded approximately 50% decrease in intimal DC accumulation, as well as 60% attenuation in plaque formation in DTR-CD11c/ApoE-/- mice after 10-day HFD. Capability of stimulating antigen-specific T cell proliferation was also impaired in aortic cells from DC-depleted mice (DT-treated: 1.62±0.30%; PBS-treated: 3.04±0.59%; p= 0.004), along with reduction in indirect conduction of T cell activation. In contrast, no significant changes were found in plaque formation and DC accumulation in DT-injected DTR-CD11b/ApoE-/- mice after 10 days of HFD, compared to control group. Furthermore, depletion of CD11b+ macrophages in either aortas or spleens didn’t alter capability of inducing antigen-specific T cell proliferation in DT-injected mice. Conclusions: These results suggested that vascular DCs rather than macrophages play a more important role in T cell activation and initiation of atherosclerosis.
Abstract 9632: A Functional Role of Tenascin-C in Angiotensin II-Induced Cardiac Fibrosis
Authors: Naoshi Shimojo, Ryotaro Hashizume, Kazuki Kanayama, Yuka Suzuki, Mari Hara, Tomohiro Nishioka, Toshimichi Yoshida, Kyoko Imanaka-Yoshida
Journal: Circulation (2014)

Introduction:  tenascin-C is sparsely detected in normal adult heart but becomes expressed in the pathological myocardium closely associated with inflammation and tissue remodeling. We have previously reported about enhanced TN-C production and macrophages accumulation in the perivascular fibrosis of angiotensin II (AngII)-induced mouse hypertensive heart.   Hypothesis:  The aim of study was to clarify the role of TN-C in progression of hypertensive cardiac fibrosis using TN-C knock-out (TNKO) mouse, and to evaluate whether TN-C is involved in macrophage activation.   Methods:  Balb/c WT and TNKO mice (8 week-old) were subcutaneously implanted with an osmotic minipump which released AngII (560 ng/kg body weight/min) for 4 weeks (WT/AngII and TNKO/AngII). In vitro, to test the direct effects of TN-C on macrophage activation, macrophages were isolated from WT mice, seeded in culture well. They were subsequently analyzed using histological and molecular biological approaches.   Results and Conclusions:  AngII treatment (WT/AngII and TNKO/AngII) elevated blood pressure, heart weight/body weight ratio, atrial and brain natriuretic peptide expression level and cardiomyocyte size, compared with the saline treatment (WT/Sa and TNKO/Sa). In TNKO/AngII mice, interstitial collagen fibers and accumulation of mac-3 positive macrophages were reduced significantly compared with those of WT/AngII in the perivascular region. Additionally, mRNA expression of interleukin (IL)-6 and monocyte chemoattractant protein (MCP)-1 was decreased. Using an in vitro migration assay, TN-C accelerated macrophage migration in the presence of MCP-1. qRT-PCR analysis demonstrated that TN-C up-regulated IL-6 and MCP-1 mRNA in an NF-κB dependent manner. Interestingly, NF-κB activation and IL-6 mRNA expression were suppressed by integrin αVβ3 antagonist. In heart tissues, Accumulation of integrin αvβ3 and activation of NF-κB in macrophages were confirmed in WT/AngII mice, which are not seen in TNKO/AngII. TN-C accelerates IL-6 production through integrin αVβ3/NF-κB on macrophages and augmenting perivascular inflammation, thereby aggravating fibrosis in a hypertensive heart.
SULFONAMIDE THERAPY OF BACTERIAL ENDOCARDITIS; RESULTS IN 42 CASES
Authors: W. R. Galbreath, Edgar Hull
Journal: Annals of Internal Medicine (1943)

Article1 February 1943SULFONAMIDE THERAPY OF BACTERIAL ENDOCARDITIS; RESULTS IN 42 CASESW. R. GALBREATH, M.D., EDGAR HULL, M.D., F.A.C.P.W. R. GALBREATH, M.D.Search for more papers by this author, EDGAR HULL, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-18-2-201 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptDuring the years 1938 to 1941, inclusive, there were 67 cases of bacterial endocarditis among the patients admitted to the Charity Hospital. Forty-two of these cases received therapy with one or more of the sulfonamide drugs. This report, reviewing the 42 cases which received sulfonamide therapy, is made in order to add data to those recorded by numerous authors relating to the effectiveness of the sulfonamide drugs in bacterial endocarditis.Criteria for Diagnosis. The diagnosis was based either upon (1) a compatible clinical picture plus one or more positive blood cultures, or (2) necropsy evidences. In 32 cases the diagnosis... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: New Orleans, Louisiana*Received for publication February 6, 1942.From the Departments of Medicine of the Louisiana State University School of Medicine and the Charity Hospital of Louisiana in New Orleans. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byAntimicrobial treatment of infective endocarditis caused by viridans streptococci highly susceptible to penicillin: historic overview and future considerationsThe Importance of Bactericidal Drugs: Future Directions in Infectious DiseaseInfektiöse EndokarditisBacterial endocarditis due to salmonella typhimuriumDie Herz- und GefäßkrankheitenSALMONELLA SUBACUTE BACTERIAL ENDOCARDITIS*MAURICE RICH, M.D., F.A.C.P., EDWARD ST. MARY, M.D.Nichtrheumatische EndokarditisNichtrheumatische EndokarditisSubacute bacterial endocarditis of nonstreptococcic etiologyThe dentist's responsibility in the management of the patient with rheumatic heart disease 1 February 1943Volume 18, Issue 2Page: 201-203KeywordsBloodDrug therapyEndocarditisHospital medicineSulfonamide ePublished: 1 December 2008 Issue Published: 1 February 1943 PDF downloadLoading ...
Abstract 14149: Daily Physical Activity in Patients After a Fontan Operation Improves Functional Capacity, Which is Related to Endothelial Function and Skeletal Muscle Hemodynamics
Authors: Kei Inai, Eriko Shimada, Tokuko Shinohara, Toshio Nakanishi
Journal: Circulation (2011)

Background: Physical activity has well-documented positive effects on endothelial function and exercise capacity in various heart diseases. We previously reported that exercise capacity in patients...