Abstract 567: Multiparametric analysis of an ultra-large prostate cancer tissue microarray with attached histo-pathological, clinical, and molecular data: A tool for rapid evaluation of clinically relevant gene sets
Authors: Martina Kluth, Maria Christina Tsourlakis, Claudia Hube-Magg, Christoph Burdelski, Katharina Grupp, Hartwig Huland, Hans Heinzer, Markus Graefen, Stefan Steurer, Ronald Simon, Thorsten Schlomm, Guido Sauter, Sarah Minner
Journal: Cancer Research (2014)

Abstract A magnitude of candidate prognostic markers has been suggested in prostate cancer during the last decade. However, virtually none of these markers has been proven to be sufficiently reliable to justify development a new routine diagnostic applications. It is hoped that combinations of multiple markers will allow for an improved prediction of the clinical behavior of theses tumors. In order In order to create a platform for rapid validation of the clinical impact of a large number of candidate prognostic markers and their combinations, we have constructed a tissue microarray containing 12,427 individual prostate cancers undergoing radical prostatectomy between 1992 and 2012, and assembled a database with detailed histological, pathological, and clinical follow-up data. More than 60 molecular parameters have been analyzed by means of immunohistochemistry (IHC) and/or fluorescence in situ hybridization (FISH) in this cancer set until today, including such that have been suggested for candidate prognosticator before as well as novel markers. Integrated analysis of these markers revealed prognostic relevant patterns of specific alterations, including a 10-genes classifier that predicted biochemical recurrence independently from established prognostic factors pathological stage, nodal stage, pre-surgical prostate specific antigen levels, Gleason grade, and status of the resection margin. We also found that the number of deletions per cancer had strong prognostic relevance. In summary, our data demonstrate the power of our prostate cancer tissue microarray platform for long term analysis of dozens of putative prognosticators on the DNA and protein level on the same tissue set with detailed clinical characterization. Despite only one tissue core per tumor has been sampled, this platform has proven to be instrumental for validation of the clinical impact of known and suggested prognosis markers. The very high number of individual samples even allows for multiparametric analysis resulting in the identification of marker sets that are superior to established prognostic parameters. Citation Format: Martina Kluth, Maria Christina Tsourlakis, Claudia Hube-Magg, Christoph Burdelski, Katharina Grupp, Hartwig Huland, Hans Heinzer, Markus Graefen, Stefan Steurer, Ronald Simon, Thorsten Schlomm, Guido Sauter, Sarah Minner. Multiparametric analysis of an ultra-large prostate cancer tissue microarray with attached histo-pathological, clinical, and molecular data: A tool for rapid evaluation of clinically relevant gene sets. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 567. doi:10.1158/1538-7445.AM2014-567