tbuckley/cabot-search huggingface dataset:
Abstract 5589: Molecular diagnostic, prognostic and therapeutic role of mir-221 in pancreatic cancer
Authors: Seema Sethi, Shaan Sarkar, Hala Dubaybo, Shadan Ali, Priscila Goncalves, Spilakshmi Kollepara, Philip Philip, Yiwei Li
Journal: Cancer Research (2014)

Abstract Background: Comprehensive molecular profiling of microRNAs (miRNAs) and subsequent target gene analysis of pancreatic cancer (PaCa) can provide tumor specific miRNAs signatures to improve diagnostic accuracy, refine prognostic and predictive capabilities, and may also serve as therapeutic targets. In PaCa such a comprehensive analysis has not been reported.Design: RNA extracted from scant amounts of formalin fixed paraffin embedded PaCa tumor and normal pancreatic tissues were profiled for miRNA expression using microfluidic biochip microarrays (which interrogate 2019 unique mature miRNAs,LC Sciences). The expression of abnormal miRNAs was then validated and quantified using real time RT-PCR. Data was statistically analyzed using the Student's t-test. Survival data obtained in each case was correlated with the miRNA data using Kaplan-Meier analysis. Oncogenic potential, therapeutic modulation and downstream target genes of altered miRNA were evaluated by real time RT-PCR using PaCa cells for mRNA expression.Results: miRNA profiling showed high expression of miR-221 in PaCa tissues compared to normal pancreas (p=0.001). These findings were validated and quantified using qRT- PCR (p=0.0029) . Survival analysis using Kaplan-Meier, demonstrated shorter survival of patients with higher expression of miR-221 compared to those with relatively lower levels of miR-221 . The cell proliferation index of PaCa cells was increased by miR-221 mimics transfection and decreased by miR-221 inhibitors. The predicted target genes of miR-221 identified by RT-PCR were PTEN, p27kip1, p57kip2 and PUMA.Conclusions: High throughput miRNA expression profiling showed high expression of miR-221 in PaCa tissues. Higher levels of miR-221 demonstrated poorer prognosis, suggesting the oncogenic potential of miR-221 in PaCa. Its target genes are PTEN, p27kip1, p57kip2 and PUMA which are tumor suppressors, and found to be deregulated by over-expression or under-expression of miR-221 transfection studies in PaCa cells. These results provide molecular evidence of oncogenic potential of miR-221 in PaCa which may have a significant clinical impact on prognosis &amp; risk stratification and in designing novel targeted molecular therapeutics in the future to achieve the goal of personalized and precision medicine. Citation Format: Seema Sethi, Shaan Sarkar, Hala Dubaybo, Shadan Ali, Priscila Goncalves, Spilakshmi Kollepara, Philip Philip, Yiwei Li. Molecular diagnostic, prognostic and therapeutic role of mir-221 in pancreatic cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5589. doi:10.1158/1538-7445.AM2014-5589
664. Surface-Expressed Angiotensin-Converting Enzyme as a Novel Approach To Target Adenovirus to Dendritic Cells
Authors: Kenichi Ogawara, Marianne G. Rots, Robbert J. Kok, Dirk K. F. Meijer, Grietje Molema, Hidde J. Haisma
Journal: Molecular Therapy (2004)

Purpose: One of the major limiting factors for gene therapy is the lack of efficiency of gene transfer to desired disease-associated cell types. To overcome this problem, we have used a novel method for modifying intrinsic tropism of adenovirus and enhancing gene delivery to vascular endothelial cells.
Abstract 4134: Prevalence of cervical cancer and associated mortality in Grenada: A ten-year analysis (2000 - 2010)
Authors: Avi' A T Bahadoor - Yetman, Laura Riley, Ashley Gibbons, Paul J. Fields, Veronica Mapp-Alexander, Robert Hage, Amy Baldwin
Journal: Cancer Research (2014)

Abstract According to recent WHO estimates, cervical cancer is ranked as the second most frequently diagnosed cancer among women ages 15 - 44 years in the Caribbean and disproportionately affects developing countries. Globally, 80 to 85% of deaths occur in low- and middle-income countries. However, little is known about cervical cancer burden in much of the Eastern Caribbean and particularly for Grenada, West Indies. Grenada is a Tri-Island state comprised of Grenada, Carriacou and Petite Martinique, with a population of 35,000 women ages 15 years and older who are at risk of developing cervical cancer. To investigate cervical cancer prevalence and its associated mortality, a study was conducted on de-identified records obtained from the Grenada General Hospital Histopathology Laboratory of women who visited hospital and clinic facilities during 2000 - 2010. Ten years of hand-written records (12,016 entries) were compiled into an electronic master sheet, and cervical cancer prevalence was assessed. Of the 12,016 entries, 9,003 were female (7,160 individuals) between ages 8 - 94 years. Sampling without replacement resulted in inclusion of 2,524 entries for analysis. Identified cancer cases were matched to corresponding patient data from death registries collected at the Ministry of Health and used to identify and calculate associated mortality rates. The highest rates of cervical cancer occurred in the 35 - 44 year age group followed by the 45 - 64 age group. Prevalence rate of cervical cancer was determined as 52.4 per 100,000 women (ages 15 and above). A total of 65 deaths attributable to cervical cancer occurred during 2000 - 2010, with women over 65 years old experiencing a majority of these deaths. The mortality rate was determined to be 16.7 per 100,000, almost twice the rates estimated by WHO for the region or reported for the previous decade (9.7 per 100,000). This study reports data that demonstrate the need for a comprehensive cervical cancer-screening program in Grenada. It should be used in the generation of public health policy and should aid future studies in determining how to appropriately execute public health policy for screening, prevention, and control of cervical cancer in Grenada. Citation Format: Avi' A T Bahadoor - Yetman, Laura Riley, Ashley Gibbons, Paul J. Fields, Veronica Mapp-Alexander, Robert Hage, Amy Baldwin. Prevalence of cervical cancer and associated mortality in Grenada: A ten-year analysis (2000 - 2010). [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4134. doi:10.1158/1538-7445.AM2014-4134
PROGRESSIVE (DISSEMINATED) COCCIDIOIDOMYCOSIS: REPORT OF A CASE
Authors: Charles D. Marple
Journal: Annals of Internal Medicine (1945)

Case Reports1 August 1945PROGRESSIVE (DISSEMINATED) COCCIDIOIDOMYCOSIS: REPORT OF A CASECHARLES D. MARPLECHARLES D. MARPLESearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-23-2-240 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptCoccidioidomycosis, which is still occasionally and erroneously referred to as a "tropical disease," is an infection caused by the fungusCoccidioides immitis(Rixford and Gilchrist, 1896) and occurs endemically in the San Joaquin Valley of California and over large areas in the Southwest. The disease, both in its benign primary form and in the fortunately uncommon disseminated form, has become thoroughly familiar to military medical officers stationed in these areas during the present war. It is reasonable to believe that physicians in other regions of the United States will encounter occasional cases of coccidioidomycosis when men who have been stationed...Bibliography1. SMITH CE: Coccidioidomycosis, Med. Clin. N. Am., 1943, xxvii, 790. CrossrefGoogle Scholar2. COURVILLEABBOTT CBKH: Pathology of coccidioidal granuloma of the central nervous system and its envelopes, Bull. Los Angeles Neurol. Soc., 1938, iii, 27-41. Google Scholar3. JACOBSON HP: Immunotherapy for coccidioidal granuloma; report of cases, Arch. Dermat. and Syph., 1939, xl, 521-540. CrossrefGoogle Scholar4. EMMONSASHBURN CWLL: The isolation of Haplosporangium parvum n. sp. and Coccidioides immitis from wild rodents. Their relationship to coccidioidomycosis, Pub. Health Rep., 1942, lvii, 1715. CrossrefGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: CHARLES D. MARPLE*Received for publication March 31, 1944.From the Medical Department of the California State Prison, San Quentin, California. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byDie MykosenDie MykosenUrogenital Aspects of Coccidioidomycosis: Review of the Literature and Report of two CasesDisseminated coccidioidomycosis 1 August 1945Volume 23, Issue 2Page: 240-249KeywordsArmed forcesCoccidioidesCoccidioidomycosisInfectious diseasesMetastasisSurgeonsTropical diseases ePublished: 1 December 2008 Issue Published: 1 August 1945 PDF downloadLoading ...
The Surgery of Infancy and Childhood: Its Principles and Techniques
Authors: Robert E. Gross
Journal: JAMA (1953)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
The ED and the Uninsured—Reply
Authors: Tracy Hampton
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Interaction of Polymorphonuclear Neutrophils with Escherichia Coli
Authors: Mark J. Bergman, Richard L. Guerrant, Ferid Murad, Stephen H. Richardson, David Weaver, Gerald L. Mandell
Journal: Journal of Clinical Investigation (1978)

Enterotoxigenic Escherichia coli are associated with noninflammatory diarrhea and stimulate adenylate cyclase activity of mammalian cells, thereby increasing intracellular cyclic adenosine 3′,5′-monophosphate (cyclic AMP). Increased concentrations of cyclic AMP in polymorphonuclear neutrophils (PMN) inhibit phagocytosis, candidacidal activity, granule discharge, and chemotactic responsiveness. We examined the effect of enterotoxin on the interaction of human PMN with E. coli. Enterotoxigenic and nonenterotoxigenic strains, including serotypes of E. coli identical except for the presence or absence of the plasmid coding for enterotoxin production, were utilized. Enterotoxigenic and nonenterotoxigenic E. coli, tumbled with PMN, were phagocytized and killed (>97%) equally well, and these strains stimulated PMN hexose monophosphate shunt activity equivalently.
574. Systemic IL-12 Eliminates Pre-Established Leukemia in a Murine Model of Philadelphia Chromosome Positive Acute Lymphoblastic Leukemia
Authors: Tanja A. Gruber, Dianne C. Skelton, Karen Pepper, Denise Peterson, Donald B. Kohn
Journal: Molecular Therapy (2004)

Acute Lymphoblastic Leukemia (ALL) is the most frequent type of leukemia in children and represents roughly 30% of all childhood malignancies. With current chemotherapy protocols event-free survival in children with ALL has reached 75%. The 25% of patients who relapse can often be predicted based on certain prognostic factors including infants less than one year of age and/or the presence of the Philadelphia chromosome (Ph+). Our laboratory has established a murine model of Ph+ ALL utilizing a cell line, BM185, generated by the transformation of Balb/c bone marrow with the Bcr-Abl oncogene. Balb/c mice injected with as few as one thousand BM185 cells die within three to four weeks of challenge, with heavy tumor burdens in the blood, spleen, and bone marrow. Irradiated BM185 cells transduced with various immunomodulators including CD80, CD40 Ligand, and GM-CSF, are able to initiate anti-leukemic immune responses, and can prevent the development of leukemia in a portion of mice when given prior to challenge with live wild type leukemia. Recently our lab initiated experiments to determine if systemic recombinant murine IL-12 (rmIL-12) injections could be used as an adjuvant to enhance the effects of our tumor cell vaccine based approach. We found that rmIL-12 injections alone were able to eliminate disease in mice given lethal doses of leukemia on the same day as the initiation of treatment. This protection was mediated by CD4 T lymphocytes, CD8 T lymphocytes, and Natural Killer cells in combination, rather than by stimulating a single cell type alone as demonstrated by depletion studies and studies done in Nude mice. Mice surviving challenge as a result of concomitant treatment with rmIL-12 did not generate immunologic memory responses, as demonstrated by rechallenge with wild type BM185 cells in long term survivors. The combination of rmIL-12 treatments and cellular vaccines consisting of irradiated BM185 cells expressing CD80, CD40 Ligand, and GM-CSF was able to provide immediate protection as well as long term memory. Currently our lab is conducting experiments to compare the efficacy of rmIL-12 systemic treatments to IL-12 transduced BM185 cells in order to establish the best mode of immune system stimulation with the least amount of toxicity. The results of IL-12 in our murine model are encouraging as we were previously unable to eliminate pre-established disease in this aggressive model of Ph+ ALL. These studies validate the feasibility of a clinical trail to evaluate the ability of vaccinations in combination with IL-12 to eliminate minimal residual disease in Ph+ ALL patients in remission.
Advanced glycation endproducts induce fibrogenic activity in nonalcoholic steatohepatitis by modulating TNF-α-converting enzyme activity in mice
Authors: Joy X. Jiang, Xiangling Chen, Hiroo Fukada, Nobuko Serizawa, Sridevi Devaraj, Natalie J. Török
Journal: Hepatology (2013)

Advanced glycation endproducts (AGEs) accumulate in patients with diabetes, yet the link between AGEs and inflammatory and fibrogenic activity in nonalcoholic steatohepatitis (NASH) has not been explored. Tumor necrosis factor alpha (TNF-α)-converting enzyme (TACE) is at the center of inflammatory processes. Because the main natural regulator of TACE activity is the tissue inhibitor of metalloproteinase 3 (Timp3), we hypothesized that AGEs induce TACE through nicotinamide adenine dinucleotide phosphate reduced oxidase 2 (NOX2); and the down-regulation of Sirtuin 1 (Sirt1)/Timp3 pathways mediate fibrogenic activity in NASH. The role of NOX2, Sirt1, Timp3, and TACE was evaluated in choline-deficient L-amino acid defined (CDAA) or Western diet (WD)-fed wild-type (WT) and NOX2−/− mice. To restore Timp3, mice were injected with adenovirus (Ad)-Timp3. Sirt1 and Timp3 expressions were studied in livers from NASH patients, and we found that their levels were significantly lower than in healthy controls. In WT mice on the CDAA or WD, Sirt1 and Timp3 expressions were lower, whereas production of reactive oxidative species and TACE activity significantly increased with an increase in active TNF-α production as well as induction of fibrogenic transcripts. Ad-Timp3 injection resulted in a significant decline in TACE activity, procollagen α1 (I), alpha smooth muscle actin (α-SMA) and transforming growth factor beta (TGF-β) expression. NOX2−/− mice on the CDAA or WD had no significant change in Sirt1, Timp3, and TACE activity or the fibrosis markers assessed. In vitro, AGE exposure decreased Sirt1 and Timp3 in hepatic stellate cells by a NOX2-dependent pathway, and TACE was induced after exposure to AGEs. Conclusion: TACE activation is central to the pathogenesis of NASH and is mediated by AGEs through NOX2 induction and down-regulation of Sirt1/Timp3 pathways. (Hepatology 2013;58:1339–1348)
Preoperative pulmonary rehabilitation in female patients undergoing a thoracotomy for lung cancer
Authors: Natasa Mujovic, Nebojsa Mujovic, Dragan Subotic, Maja Ercegovac, Ljubica Nikcevic
Journal: European Respiratory Journal (2014)

Objectives: during the last decade, a significant increase in lung cancer among females has been registered. The aim of the study was to assess the impact of respiratory rehabilitation to the lung function and effort tolerance preoperatively and one month after the operation. Methods: the study included 68 female COPD patients, candidates for surgery for primary lung cancer, who underwent a program of preoperative pulmonary rehabilitation (PPR) of average duration of two weeks, consisting of aerosol-bronchodilator therapy, kinesitherapy for upper and lower limbs. The six-minutes walking test (6MWT), with a measurement of the walking distance, oxygen saturation (pO 2 ) and lung function, served as a criterion for the treatment success. All these parameters were measured on admission, before and one month after thoracotomy. Results: a significant improvement of vital capacity and forced expiratory volume in the 1 st  second after the PPR was registered (p 2  was also significant. There was no significant difference in 100FEV 1 /VC and in the small airays function. Postoperatively, a loss in values of all ventilatory parameters occurred. The absence of the shortening of the 6-min. distance mirrored the net –effect of PPR. Besides, PPR contributed to preservation of the postoperative patients9symptomatic status and to abolishment of the expected dyspnea at rest and of the effort-provoked symptoms. Conclusion: by performing PPR, an evident improvement of the lung function and effort tolerance is achieved, thus having a favorable impact to the postoperative course.
"CHOKES": A RESPIRATORY MANIFESTATION OF AEROEMBOLISM IN HIGH ALTITUDE FLYING
Authors: Ezra V. Bridge, Franklin M. Henry, Owen L. Williams, John H. Lawrence
Journal: Annals of Internal Medicine (1945)

Article1 March 1945"CHOKES": A RESPIRATORY MANIFESTATION OF AEROEMBOLISM IN HIGH ALTITUDE FLYINGEZRA V. BRIDGE, M.D., FRANKLIN M. HENRY, PH.D., OWEN L. WILLIAMS, M.D., JOHN H. LAWRENCE, M.D.EZRA V. BRIDGE, M.D.Search for more papers by this author, FRANKLIN M. HENRY, PH.D.Search for more papers by this author, OWEN L. WILLIAMS, M.D.Search for more papers by this author, JOHN H. LAWRENCE, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-22-3-398 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe tactical success of certain operations in this war has required aircraft capable of flying in the stratosphere. To keep pace with the accomplishments of the aeronautical engineer, the physiologist has had to study man's tolerance for and means of protecting him from three major hazards of the stratosphere: lack of oxygen, extreme cold, and low atmospheric pressure. The disease produced by exposure to rapid decrease in atmospheric pressure, such as encountered in the ascent of aircraft to high altitude, is called aeroembolism or decompression sickness. The manifestations of this disease are numerous. Armstrong1has grouped them in order of...Bibliography1. ARMSTRONG HG: Principles and practice of aviation medicine, ed. 2, 1943, Williams & Wilkins Company, Baltimore, pp. 354 and 366. Google Scholar2. BRIDGE EV: Decompression sickness: nature and incidence of symptoms during and after artificial decompression to 38,000 feet with exercise during exposure, Jr. Aviation Med., 1944, xv, 316-328. Google Scholar3. Unpublished observations. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Berkeley, California*Received for publication September 16, 1944.From the Aeromedical Research Laboratory, University of California, Berkeley.The work described in this paper was done under a contract recommended by the Committee on Medical Research between the Office of Scientific Research and Development and the University of California. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 March 1945Volume 22, Issue 3Page: 398-407KeywordsEngineersOxygenResearch laboratories ePublished: 1 December 2008 Issue Published: 1 March 1945 PDF downloadLoading ...
Clinical, molecular and biochemical characterization of nine Spanish families with Conradi-Hünermann-Happle syndrome: new insights into X-linked dominant chondrodysplasia punctata with a comprehensive review of the literature
Authors: J. Cañueto, M. Girós, S. Ciria, G. Pi-Castán, M. Artigas, J. García-Dorado, V. García-Patos, A. Virós, T. Vendrell, A. Torrelo, Á. Hernández-Martín, E. Martín-Hernández, M.T. Garcia-Silva, M. Fernández-Burriel, J. Rosell, M. Tejedor, F. Martínez, J. Valero, J.L. García, E.M. Sánchez-Tapia, P. Unamuno, R. González-Sarmiento
Journal: British Journal of Dermatology (2011)

Conradi-Hünermann-Happle syndrome (CDPX2, OMIM 302960) is an inherited X-linked dominant variant of chondrodysplasia punctata which primarily affects the skin, bones and eyes. CDPX2 results from mutations in EBP (emopamil binding protein), and presents with increased levels of sterol precursors 8(9)-cholesterol and 8-dehydrocholesterol.To expand the understanding of CDPX2, clinically, biochemically and genetically.We present one of the largest series reported to date, including 13 female patients belonging to nine Spanish families. Patients were studied biochemically using gas chromatography-mass spectrometry, genetically using polymerase chain reaction and in their methylation status using the HUMARA assay.In our cases, there was a clear relationship between abnormal sterol profile and the EBP gene mutation. We describe three novel mutations in the EBP gene. EBP mutations were inherited in three out of nine families and were sporadic in the remaining cases.No clear genotype-phenotype correlation was found. Patients' biochemical profiles did not reveal a relationship between sterol profiles and severity of disease. A skewed X-chromosome inactivation may explain the clinical phenotype in CDPX2 in some familial cases.