tbuckley/cabot-search huggingface dataset:
Abstract 20166: Obesity-Related Changes in Circulating Microrna Profile After Standardized Meal
Authors: Tamas Alexy, Kimberly Rooney, Ngoc-Anh Le, Arnold H Slyper, Charles D Searles
Journal: Circulation (2014)

Background:  MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally silence gene expression. While primarily intracellular, miRNAs are detectable in the human blood where they potentially serve as intercellular communicators. Recent evidence suggests that circulating miRNA levels may be affected by dietary factors. However, little is known about the acute effects of a meal challenge on plasma miRNA profiles and its relationship with inflammatory biomarkers in young adults with normal and elevated BMI.   Methods:  We enrolled 10 obese (BMI: 38.9±6.5 kg/m 2 ;  average age: 18.6±3.0 years) and 10 control (BMI: 21.7±1.9 kg/m 2 ; average age: 18.3±3.3 years) males in our case-control study where participants consumed a standardized low-fat mixed meal after 12 hours fast. Venous blood was collected at baseline and at 2, 4 and 6 hours and the expression of the following miRNAs was determined using qRT-PCR and normalized to total RNA: miR-126, -21, -155, -222, -223, -122, -145, -346, -191 and 520-5p. In addition, levels of TNF-α, E-selectin and hs-CRP were measured at 0 and 4 hours.   Results:  We found significantly elevated circulating miR-155 expression in obese individuals at baseline (p   Conclusions:  Our study demonstrates the effect of diet on circulating miRNA profiles and emphasizes the importance of standardized, fasting blood collection for circulating miRNA profiling. Our data also demonstrate that circulating miRNAs are very early markers of the inflammatory post-prandial response, and this response is dysregulated in young, obese individuals. These findings have implications for the increased long-term cardiovascular risk in this population.
Tamoxifen and (4-benzyl-phenoxy)-ethyl-N-pyrrolidine (PBPE), a selective ligand of the microsomal antiestrogen binding site (AEBS) induced type II apoptosis or autophagy in tumor cell lines.
Authors: Philippe De Médina, Bruno Payré, Nadia Boubekeur, Gilles Favre, Sandrine Silvente-Poirot, Marc E. Poirot
Journal: Cancer Research (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

5516 

(4-benzyl-phenoxy)-ethyl-N-pyrrolidine,HCl (PBPE) is a non-antiestrogen derivative of the antitumoral drug tamoxifen. PBPE is a selective and high affinity ligand of the microsomal antiestrogen binding site (AEBS). We report that PBPE is cytotoxic on tumor cell lines of various origins in a concentration-dependent manner. The potency of PBPE is equivalent to that of tamoxifen. The apoptotic nature of the toxicity induced in MCF-7 by PBPE was characterized by: 1) the condensation of the heterochromatin, 2) the binding annexin-V, 3) the appearence of inter-nucleosomal DNA cleavage, 4) a strong diminution of the Bcl2/Bax ratio, 5) the release of cytochrome C in the cytoplasm. The cytoxicity induced by PBPE, involved both the transcription and the translation of genes, is blocked by vitamine E and C but not by caspase inhibitors, suggesting a caspase-independent active cell death. PBPE and tamoxifen induced a nuclearization the mitochondrial AIF factor that can explain the activation of nucleases. Moreover PBPE and tamoxifen stimulate the accumulation of autophagic vacuoles, the appearence of autophagosomes, the expression of the protein Beclin and the proteolysis of the LC3 protein in cells. Altogether these data demonstrate that PBPE, a non antiestrogen derivative of tamoxifen displayed a high cytotoxic potency on tumoral cells. Moreover we show that PBPE and tamoxifen triggered type II apoptosis or autophagy through a common pathway involving the AEBS.
A comparison of patterns of sun protection during beach holidays and everyday outdoor activities in a population sample of young German children
Authors: J. Li, W. Uter, A. Pfahlberg, O. Gefeller
Journal: British Journal of Dermatology (2012)

Reducing exposure to ultraviolet (UV) radiation is the main effective measure for preventing skin cancer. Educational campaigns targeting sun protection have been focused either on behaviour on the beach during the summer holiday alone, or during everyday outdoor activities of the children. Little is known about the comparison between these different settings.To analyse whether parents apply similar protective measures to reduce UV exposure for their young children in different outdoor environments.Families (n = 2619) with children aged 3-6 years (response: 64·7%) were enrolled in a population-based survey in the German city of Erlangen and its surrounding rural county. Using a self-administered standardized questionnaire parents gave information about demographic and photosensitivity data of their children, their knowledge about risk factors for skin cancer and their typical instructions given to their children when these played outside on a summer day in different outdoor environments.Significant discrepancies regarding the four UV protective measures (clothes, shade, sunhat, sunscreen) for children between an everyday outdoor setting and a holiday setting on the beach were observed. A high level of parental risk factor knowledge was significantly associated with a better protection for children in all four measures only on the beach. Photosensitivity and demographic characteristics had some impact on protective behaviour, too. Measures of sun protection were reduced with children's increasing age.Skin cancer prevention campaigns should target the encouragement of sun protection for children also in outdoor activities of daily living, not only during a summer holiday on the beach.
Abstract 4106: Curcumin inhibit PhIP induced cytotoxicity by inhibiting ROS production, DNA strand breaks and DNA adducts formation in MCF 10A cells
Authors: Ashok K. Jain, Abhilash Samykutty, Carissa L. Jackson, Muthusamy Thangaraju, Darren D. Browning
Journal: Cancer Research (2014)

Abstract PhIP is a known food carcinogen found in well done meat which causes several cancers including breast cancer. PhIP metabolites produce DNA adduct and DNA strand breaks. Curcumin, obtained from the rhizome of Curcuma longa, has potent anticancer activity. So far none of the study demonstrates the inhibition of PhIP induced cytotoxicity by the co-treatment of Curcumin in normal breast epithelial cells in vitro. Therefore, we developed a model system using the MCF 10A normal breast epithelial cells to study the PhIP cytotoxicity and if cells are co-cultured with Curcumin and PhIP how it affects. Consequently, the core signaling pathways have been explored to evaluate the efficacy of Curcumin. In this study, the effectiveness of Curcumin was investigated in MCF 10A cells along with PhIP. The cytotoxic ability was detected with MTT assay, ROS activation by DCF, the influence of the cell cycle was checked with flow cytometry, DNA damage by comet assay, DNA adduct formation by anti-PhIP DNA primary, and apoptosis by Annexin-V-FITC staining. The influence of the core signaling pathways was evaluated by RT PCR and/or Western blotting which included Nrf2 (GSR, GPX, NQO1), FOXO (Catalase, GADD45, PRDX3) targets; DNA repair genes/proteins BRCA1, H2AFX and PARP-1; and tumor suppressor P16 gene expression. PhIP cytotoxicity induced the expression of various antioxidant and DNA repair genes on MCF-10A cells but co-treatment of Curcumin retained its expression level similar to untreated groups. Additionally, Curcumin co- treatment increased the expression level of tumor suppressor expression gene P-53. Expression of antioxidants genes was induced by PhIP whereas Curcumin significantly suppress the PhIP induced ROS activation, DNA strand breaks and DNA adduct formation and consequently inhibited the cell death. In conclusion, Curcumin appears to be effective to inhibit P450 mediated ROS production and PhIP-DNA adducts which consequently reduces DNA damage. Citation Format: Ashok K. Jain, Abhilash Samykutty, Carissa L. Jackson, Muthusamy Thangaraju, Darren D. Browning. Curcumin inhibit PhIP induced cytotoxicity by inhibiting ROS production, DNA strand breaks and DNA adducts formation in MCF 10A cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4106. doi:10.1158/1538-7445.AM2014-4106
OCCULT SUBACUTE BACTERIAL ENDOCARDITIS MANIFESTED BY HEMATOLOGIC ABNORMALITIES, INCLUDING PANCYTOPENIA
Authors: W B PARSONS, T COOPER, C H SCHEIFLEY
Journal: Annals of Internal Medicine (1953)

Article1 August 1953OCCULT SUBACUTE BACTERIAL ENDOCARDITIS MANIFESTED BY HEMATOLOGIC ABNORMALITIES, INCLUDING PANCYTOPENIAWILLIAM B. PARSONS JR., M.D., TALBERT COOPER, M.D., F.A.C.P., CHARLES H. SCHEIFLEY, M.D., F.A.C.P.WILLIAM B. PARSONS JR., M.D., TALBERT COOPER, M.D., F.A.C.P., CHARLES H. SCHEIFLEY, M.D., F.A.C.P.Author, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-39-2-318 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptAnemia of variable degree is the commonest hematologic abnormality observed in subacute bacterial endocarditis. The erythrocytes are usually somewhat hypochromic but may be normochromic. Usually the leukocytes are normal in number, but they may be increased, particularly if embolic complications are present. Morphologically, "toxic" changes are apparent in the granulocytes, sometimes accompanied by a shift to the left. The number of platelets has not been consistently altered in cases reported in the literature. The presence of phagocytic reticuloendothelial cells in stained smears of peripheral blood has been demonstrated by many observers in about 15 per cent of cases. Although these...Bibliography1. Sturgis CC: Hematology, 1948, Charles C. Thomas, Springfield, Illinois. Google Scholar2. ParsonsCooperScheifley WBTCH: The anemia of bacterial endocarditis, unpublished data. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Rochester, Minnesota*Received for publication March 27, 1953.From the Mayo Foundation and the Mayo Clinic, Rochester, Minnesota. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byMultiorgan Involvement Confounding the Diagnosis of Bartonella henselae Infective Endocarditis in Children With Congenital Heart DiseaseMycobacterial disease associated with aplastic anaemiaAplastic anemia in Shiraz, an analysis of 50 cases with special reference to etiological agents in Southwest IranErkrankungen des EndokardCase 43062Coexistent Aplastic Anaemia and Coarctation of the AortaErrors in the Recognition and Treatment of Heart DiseaseCase 39462 1 August 1953Volume 39, Issue 2Page: 318-325KeywordsAnemiaBlood cellsCellsEndocarditisGranulocytesPlatelets ePublished: 1 December 2008 Issue Published: 1 August 1953 PDF downloadLoading ...
Abstract 16294: Chronic Alcohol Feeding Increases miR-214 and Down regulates Bcl-2 in Heart with Asymptomatic Cardiac Dysfunction
Authors: Arun Samidurai, Lei Xi, Anindita Das, Fadi N Salloum, Rakesh C Kukreja
Journal: Circulation (2014)

Background: Abusive chronic alcohol consumption over a long period can cause metabolic changes in heart and is a risk factor for non-ischemic cardiomyopathy. Alcohol abusers have no clinical manifestation and remain asymptomatic until challenged with stress, such as cardiac ischemia. Recent study shows MicroRNA-214 (miR-214) as an excellent sensor of underlying stress signals that leads to harmful effects. Therefore we designed this chronic study in mice to diagnose the changes in miR-214 level and its targets and use sildenafil, a cardio protective drug to prevent the heart from alcohol induced injury. Methods and Results: Adult male 12-14 weeks old FVB mice were grouped to receive unlimited access to normal liquid diet as control (n =6) or Alcohol diet (35 % of daily energy intake, n=7) or alcohol with sildenafil (1mg/kg ;n=6) for 14 weeks. Animal groups fed with alcohol or sildenafil with alcohol diet showed increased volume consumption and a loss in body weight (Fig.1&2). Echocardiography showed no si...
Clinical significance of alpha-catenin, collagen IV, and Ki-67 expression in epithelial ovarian cancer.
Authors: M Anttila, V M Kosma, H Ji, X Wei-Ling, J Puolakka, M Juhola, S Saarikoski, K Syrjänen
Journal: Journal of Clinical Oncology (1998)

PURPOSE To analyze alpha-catenin and collagen IV expression in epithelial ovarian cancer with special reference to their prognostic significance and correlations with clinical and pathologic characteristics, as well as cell proliferation marker Ki-67. PATIENTS AND METHODS Alpha-catenin, collagen IV, and Ki-67 expression was immunohistochemically analyzed in paraffin-embedded specimens of 316 patients with epithelial ovarian cancer. RESULTS Alpha-catenin and collagen IV expression was not interrelated or related to International Federation of Gynecology and Obstetrics (FIGO) stage or proliferation marker Ki-67. Alpha-catenin expression was reduced (&lt; 100%) in 50% of primary tumors. Reduced alpha-catenin and collagen IV expression was directly related to high histologic grade (P &lt; .001). In both univariate and multivariate analyses, Ki-67 proliferation significantly predicted overall survival. In the subset of 86 patients with stage I tumor, a reduced (&lt; 100%) alpha-catenin expression approached statistical significance as a negative prognostic factor (P = .035) and retained its statistical significance in the multivariate analysis (P = .025). The low (&lt; 30%) expression of alpha-catenin (n = 10) was a sign of inferior survival as compared with normal expression in both the univariate (P = .0107) and multivariate analyses (P = .0105). CONCLUSION Alpha-catenin expression seems to be a useful marker of those FIGO stage I tumors likely to run a less favorable course. The high cell proliferative activity was associated with poor survival. In the future, alpha-catenin and Ki-67 expression should be studied in a large prospective cohort that includes early-stage cancers to select the more aggressive tumors for intense early chemotherapy.
Lost by Degrees
Authors: S Whiteman
Journal: JAMA (2000)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Mutations in SLC19A2 cause thiamine-responsive megaloblastic anaemia associated with diabetes mellitus and deafness
Authors: Valentina Labay, Tal Raz, Dana Baron, Hanna Mandel, Hawys Williams, Timothy Barrett, Raymonde Szargel, Louise McDonald, Adel Shalata, Kazuto Nosaka, Simon Gregory, Nadine Cohen
Journal: Nature Genetics (1999)
Abstract 487: Heart fibroblasts mediate the effect of High Mobility Group Box 1 Protein on cardiac stem cells
Authors: Alessandra Rossini, Antonella Zacheo, David Mocini, Pierangela Totta, Antonio Facchiano, Paolo Sordini, Raffaella Castoldi, Giulio Pompilio, Maurizio C. Capogrossi, Antonia Germani
Journal: Circulation (2007)
Post-Vaccination Encephalitis
Authors: Charles Armstrong
Journal: Annals of Internal Medicine (1931)

Article1 September 1931Post-Vaccination EncephalitisCHARLES ARMSTRONGCHARLES ARMSTRONGSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-5-3-333 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptPost-vaccination encephalitis is a disease of unknown etiology that has appeared in recent years and which occurs without regard to the existence of known factors other than the presence of a recent vaccination against smallpox. The disease has a mortality rate of approximately 43 per cent and has associated with it pathological changes of the central nervous system which are similar to those found in the acute nervous manifestations occasionally noted following smallpox, chicken pox, measles and certain other acute infections.HISTORY AND EPIDEMIOLOGYLucksch4in June, 1924, published a series of three cases with post-vaccination nervous manifestations which occurred...References1 GRÜNEBERG : Cited from Hekman, 1930. Google Scholar2 HEKMAN J: L'encéphalite postvaccinale et son traitement par le sérum homologue, Bull. Acad. de Méd., 1930, ciii, 539-551. Google Scholar3 HORDER T: Case of cerebral symptoms following vaccination; rapid recovery after intrathecal injection of post-vaccinal serum, Lancet, 1929, xi, 1301. CrossrefGoogle Scholar4 LUCKSCH F: Vaccination and encephalitis, Med. Klin., 1924, xx, 1170-1171. Google Scholar5 SCOTT TF: Post-vaccinal encephalitis in infancy, Brit. Jr. Child. Dis., 1930, xxvii, 245-269. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: U. S. Public Health Service Washington, D. C.*Presented at the Baltimore Meeting of the American College of Physicians, March 24, 1931. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byReport of a case of postvaccinal encephalitis in a four-month-oldchild with recovery 1 September 1931Volume 5, Issue 3Page: 333-337KeywordsCentral nervous systemChickenpoxDeath ratesEncephalitisEtiologyMeasles ePublished: 1 December 2008 Issue Published: 1 September 1931 PDF downloadLoading ...
336. Foamy Virus - Adenovirus Hybrid Vectors
Authors: Axel Rethwilm, Marcus Picard-Maureau, Florian Kreppel, Dirk Lindemann, Thomas Juretzek, Ottmar Herchenroeder, Martin Heinkelein, Stefan Kochanek
Journal: Molecular Therapy (2004)

Top of pageAbstract
Foamy virus (FV) infections are known to take a non-pathogenic course and the application of high-capacity (“gutless”) adenovirus (AdV) vectors has also been shown to be superior to first and second generation AdV vectors. To confer AdV vectors the feature of integration into the host cell genome hybrid vectors were characterized in vitro, which express vectors derived from the prototypic FV in the backbone of a high-capacity AdV vector. FV constitute a subfamily of retroviruses with a distinct replication pathway. In the absence of envelope glycoprotein the prototypic FV behaves like an retrotransposon, while it behaves like an exogenous retrovirus in its presence. Two principle types of vectors, which either allow the intra-cellular (HC-FAD-7) or, in addition, the extra-cellular (HC-FAD-2) pathway were constructed. In both chimeras expression of the FV vector was controlled by the tetracycline regulatable system. Hybrids were produced to close to 10E10 infectious units per ml. By Southern blotting the functionality of the hybrid vectors to generate host cell genomic integrants was shown. However, the efficiency of HC-FAD-7 to establish stable transgene expression was rather low, while around 70% of cells were stably transduced in secondary round following primary transduction with HC-FAD-2 at an MOI of 100. Given the benign characteristics of high-capacity AdV and FV vectors, hybrids based on HC-FAD-2 are probably suited for an in vivo application.
Immunotherapy with Yellow Jacket Venom
Authors: H. Mosbech, H.‐J. Malling, I. Biering, H. Böwadt, M. Søborg, B. Weeke, H. Løwenstein
Journal: Allergy (1986)

Thirty‐two patients with previous systemic allergic reaction to yellow jacket stings were randomly allocated to three groups receiving immunotherapy with different preparations of yellow jacket venom: 1) extract adsorbed to aluminium hydroxide (Alutard®‐SQ), 2) Pharmalgen® extract or 3) non‐adsorbed extract from Allergologisk Laboratorium (ALK aq.). Regular examinations showed a decrease in skin prick test size in nearly all patients. Specific IgE‐antibody (RAST and CRIE scores) showed a similar, but not significant tendency to decrease in all three groups. Specific IgG‐antibody increased considerably in the Alutard group only; after 2 years, however, no difference could be detected between the three groups. During dose increase, patients treated with ALK aq. generally had smaller local reactions to injections than those treated with Pharmalgen. Few systemic reactions occurred in all three groups. Nineteen patients treated for 21/2–31/2 years were challenged in‐hospital with stings from yellow jackets. No systemic and only minor local reactions occurred. Consequently, with the dose regimens applied all three extracts seem effective even though no common changes in either specific IgE or IgG could be demonstrated.
Fish, ω-3 Polyunsaturated Fatty Acids, and Mortality From Cardiovascular Diseases in a Nationwide Community-Based Cohort of Japanese Men and Women
Authors: Kazumasa Yamagishi, Hiroyasu Iso, Chigusa Date, Mitsuru Fukui, Kenji Wakai, Shogo Kikuchi, Yutaka Inaba, Naohito Tanabe, Akiko Tamakoshi
Journal: Journal of the American College of Cardiology (2008)
Involvement by Young Drivers in Fatal Alcohol-Related Motor-Vehicle Crashes—United States, 1982-2001
Authors: R W Elder, R A Shult
Journal: JAMA (2003)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal