tbuckley/cabot-search huggingface dataset:
Stimulatory Effect of High Polyunsaturated Fat Diet on Lung Metastasis From the 13762 Mammary Adenocarcinoma in Female Retired Breeder Rats&lt;xref ref-type="fn" rid="FN2"&gt;2&lt;/xref&gt;
Authors: E B Katz, E S Boylan
Journal: JNCI Journal of the National Cancer Institute (1987)

The effect of a high-fat (HF) diet (23% corn oil) on the growth and metastasis of the 13762 mammary tumor in Fischer 344 retired breeder (RB) and young virgin (YV) female rats was studied. The RB (10-12 mo old) and YV (8 wk old) rats were fed the HF or low-fat (LF) diet (5% corn oil) prior to and following tumor implantation for a total of at least 10 weeks. The growth rate of the primary tumor in the intact RB and YV was not affected by the HF diet. In RB rats ovariectomized 4 weeks prior to tumor implantation, the tumor grew significantly faster in the HF group as compared to the LF group. The total volume of metastatic tumor nodules in the lungs of the HF groups was significantly higher than that in the the lungs of the LF groups in both the intact and ovariectomized RB. In the YV, there was no difference in pulmonary metastatic burden between the HF and LF groups. The weights of the HF intact and ovariectomized RBs were higher than those of the LF animals. However, when pulmonary metastatic tumor burden was compared to body weight at implant or at sacrifice, there was no significant correlation in either the HF or LF groups. These results suggest that an HF diet enhanced the growth of pulmonary metastases in the intact and ovariectomized RB but not the YV rats and that the effect of the HF diet on pulmonary tumor burden cannot be attributed entirely to increased body weight.
Association of allergy with chronic sinusitis in children
Authors: Svetlana Kamenov, Snezana Cekic, Aleksandar Kamenov, Borislav Kamenov
Journal: European Respiratory Journal (2014)

Introduction: Chronic sinusitis in children is a common and troublesome disease for clinician and is frequently associated with allergy and asthma. Since there are insufficient data related to the pathogenesis of chronic sinusitis in children, an attempt was made to determine whether a correlation between allergy and the chronic sinusitis in children exists or not.  Methods: Sixty seven children with chronic sinusitis diagnosed by clinical and radiology criteria were enrolled in the study. Medical records and family histories were reviewed. Serum samples were assayed for a complete blood differential count, immunogloblins, total IgE and specific IgE, as well as prick allergy tests. Case control analysis was performed to determine possible risk factors for chronic sinusitis.  Results: The children with chronic sinusitis had a higher prevalence of allergic rhinitis (71%) and asthma (39%) than the subjects with acute sinusitis (p   or = 450/uL, adenoid hypertrophy, specific IgE and positive prick tests to inhalatory allergens were associated with chronic sinusitis. Allergic rhinitis and asthma were also associated with chronic sinusitis in children (p   Conclusions: Allergic rhinitis, asthma, specific IgE, positive prick allergy tests, eosinophilia, and adenoid hypertrophy were associated with chronic sinusitis in children, suggesting that allergy may contribute to the development of chronic sinusitis.
Use of matched or mismatched rIL-2 activated donor lymphocytes positively selected for CD56+ for immunotherapy of resistant leukemia after allogeneic stem cell transplantation
Authors: S. Slavin, S. Morecki, M. Y. Shapira, M. Bitan, S. Samuel, A. Ackerstein, Y. Gelfand, I. B. Resnick, R. Or
Journal: Journal of Clinical Oncology (2004)

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VARIATION IN THE GROUP-SPECIFIC CARBOHYDRATE OF GROUP C HEMOLYTIC STREPTOCOCCI
Authors: Richard M. Krause, Maclyn McCarty
Journal: The Journal of Experimental Medicine (1962)

Certain strains of Group C hemolytic streptococci, termed Group C-intermediate, contain a group-specific carbohydrate antigen which gives a precipitin cross-reaction with A-variant antiserum. The carbohydrate antigens of these strains have a rhamnose:hexosamine ratio ranging from 2.4 to 2.6 whereas the ratio of typical Group C strains varies between 1.1 and 1.7. N-acetylgalactosamine, the major hexosamine in all of these strains is the principle determinant of Group C specificity. The high concentration of rhamnose in the C-intermediate carbohydrate suggests that a portion of the rhamnose oligosaccharide side chains are devoid of terminal N-acetylgalactosamine and thus react with Group A-variant antiserum. This view is supported by the fact that the induced variant enzyme, which destroys A-variant carbohydrate reactivity with the liberation of rhamnose oligosaccharides, has a similar action upon the Group C-intermediate carbohydrate. C-intermediate carbohydrate, after treatment with variant enzyme which removed approximately 25 per cent of the rhamnose, does not react with A-variant antisera.
Restored Immunosuppressive Effect of Mesenchymal Stem Cells on B Cells After Olfactory 1/Early B Cell Factor–Associated Zinc‐Finger Protein Down‐Regulation in Patients With Systemic Lupus Erythematosus
Authors: Xuebing Feng, Nan Che, Yan Liu, Haifeng Chen, Dandan Wang, Xia Li, Weiwei Chen, Xiaolei Ma, Bingzhu Hua, Xiang Gao, Betty P. Tsao, Lingyun Sun
Journal: Arthritis & Rheumatology (2014)

To evaluate whether olfactory 1/early B cell factor-associated zinc-finger protein (OAZ), a candidate lupus susceptibility gene involved in antinuclear antibody (ANA) production, plays a role in the regulation of B cells by mesenchymal stem cells (MSCs).MSCs derived from the bone marrow of patients with systemic lupus erythematosus (SLE) and healthy control subjects were expanded and incubated with small interfering RNAs specific for OAZ or a nontargeting sequence. Knockdown of messenger RNA levels of OAZ and its downstream genes was measured using real-time polymerase chain reaction, and protein levels of chemokine/cytokine and immunoglobulins were determined by enzyme-linked immunosorbent assay or Western blotting. The effects of modulating the OAZ levels in MSCs, by either silencing or overexpression, on B cell proliferation and terminal differentiation were assessed by coculturing MSCs with mouse spleen cells.OAZ gene expression was highly enriched in MSCs compared with peripheral blood leukocytes and was increased in patients with SLE compared with control subjects. After the silencing of OAZ expression, SLE MSCs could regain the ability to inhibit B cell proliferation and terminal differentiation, as indicated by decreased percentages of bromodeoxyuridine-positive cells and CD138+ cells as well as decreased levels of IgG, IgM, and ANAs. The level of CCL2 was increased after OAZ knockdown, while anti-CCL2 antibodies completely counteracted the effect of OAZ silencing. Umbilical cord-derived normal MSCs that overexpressed OAZ had a diminished ability to inhibit B cell proliferation and terminal differentiation.OAZ down-regulation could restore the impaired function of SLE MSCs in the immune regulation of B cells, contributing to a reduction in ANA levels. OAZ might represent a new target for therapy in patients with SLE.
Abstract 3837: Innovative rapid gene methylation analysis of surgical margin tissues in head and neck cancer
Authors: Masamichi Hayashi, Rafael Guerrero-Preston, Zubair Kahn, Xiufeng Li, Julie Ahn, Marla Goldsmith, Christina Michailidi, Wayne Koch
Journal: Cancer Research (2014)

Abstract Background: To secure negative surgical margins is the primary goal of surgical cancer treatment. However, tumor recurrence sometimes occurs even with histologically negative surgical margins. In order to detect submicroscopic residual cancerous cells intra-operatively, a time saving molecular biological approach is required. Methods: We established an innovative rapid Quantitative methylation PCR (QMSP) assay, which consists of remarkably time-minimized DNA extraction, bisulfite treatment and QMSP quantification. In order to assess the feasibility of completing the rapid assay during a surgical procedure, 10 serial specimens of primary head and neck cancers were evaluated by both rapid QMSP and conventional QMSP. Results: The DNA product quality of the most time saving procedure was as good as that of a conventional procedure. In the QMSP assay, target gene methylation and reference gene methylation were equally detected by both rapid and conventional methods. Finally, two frequently methylated genes in head and neck cancers, homeobox A9 (HOXA9) and endothelin receptor type B (EDNRB) were analyzed in 10 head and neck tumors, margin tissues and normal tissues. Relative QMSP values of rapid and conventional QMSP were quite similar in almost all tumors, margins and normal tissues. The average total time for the rapid QMSP procedure was less than 3 hours. Although postoperative follow-up periods are less than a year, local tumor recurrence occurred in one case whose margin tissue was QMSP positive. Conclusion: This innovative rapid QMSP yields acceptable levels of methylation detection and can be completed during the operative window. The procedure promises to become a useful clinical tool, if the methylated surgical margins are revealed to correlate with the local tumor recurrence in a larger cohort. Citation Format: Masamichi Hayashi, Rafael Guerrero-Preston, Zubair Kahn, Xiufeng Li, Julie Ahn, Marla Goldsmith, Christina Michailidi, Wayne Koch. Innovative rapid gene methylation analysis of surgical margin tissues in head and neck cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3837. doi:10.1158/1538-7445.AM2014-3837
Relationship Between Tumor Location and Relapse in 6,781 Women With Early Invasive Breast Cancer
Authors: Caroline Lohrisch, Jeremy Jackson, Amanda Jones, Donna Mates, Ivo A. Olivotto
Journal: Journal of Clinical Oncology (2000)

To explore the independent prognostic impact of medial hemisphere tumor location in early breast cancer.A comprehensive database was used to review patients referred to the British Columbia Cancer Agency from 1989 to 1995 with early breast cancer. Patients were grouped according to relapse risk (high or nonhigh) and adjuvant systemic therapy received. Multiple regression analysis was used to determine whether the significance of primary tumor location (medial v lateral hemisphere) was independent of known prognostic factors and treatment.In the adjuvant systemic therapy groups, medial location was associated with a 50% excess risk of systemic relapse and breast cancer death compared with lateral location. Five-year systemic disease-free survival rates were 66.3% and 74.2% for high-risk medial and lateral lesions, respectively (P <.005). Corresponding 5-year disease-specific survival rates were 75.7% and 80.8%, respectively (P <.03). No significant differences were observed between medial and lateral location for low-risk disease regardless of adjuvant therapy or for high-risk disease with no adjuvant therapy. Local recurrence rates were similar for all risk and therapy groups.The two-fold risk of relapse and breast cancer death associated with high-risk medial breast tumors may be due to occult spread to internal mammary nodes (IMNs). Enhanced local control, such as with irradiation of the IMN chain, may be one way to reduce the excess risk. Ongoing randomized controlled trials may provide prospective answers to the question of the optimal volume of radiotherapy.
Association Between Memory Complaints and Incident Alzheimer’s Disease in Elderly People With Normal Baseline Cognition
Authors: Mirjam I. Geerlings, Cees Jonker, Lex M. Bouter, Herman J. Adèr, Ben Schmand
Journal: American Journal of Psychiatry (1999)

Results of previous studies suggest that memory complaints may predict cognitive decline and dementia among elderly people in whom cognitive impairment is already apparent. However, cognitive decline is often a gradual process, and elderly people may notice that their memory deteriorates before mental status tests are able to detect any change in cognitive functioning. Therefore, the authors hypothesized that memory complaints would predict incident Alzheimer's disease in elderly subjects with no signs of cognitive impairment.In the community-based Amsterdam Study of the Elderly, a sample of 3,778 nondemented persons, 65 to 84 years old, was selected and divided into two cognitive categories: normal (Mini-Mental State scores of 26-30) and borderline and impaired (Mini-Mental State scores less than 26). At baseline, the presence or absence of memory complaints was assessed. At follow-up, incident cases of Alzheimer's disease were diagnosed in a two-step procedure.After an average of 3.2 years, 2,169 persons were reevaluated, of whom 77 had incident Alzheimer's disease. Multivariate logistic regression analyses showed that memory complaints were associated with incident Alzheimer's disease in subjects with normal baseline cognition but not in subjects with impaired baseline cognition.The findings of this study suggest that memory complaints are a relatively strong predictor of incident Alzheimer's disease in older persons in whom cognitive impairment is not yet apparent. Furthermore, they suggest that older persons may be aware of a decline in cognition at a time when mental status tests are still unable to detect a decline from premorbid functioning.
Development and validation of an epidermolysis bullosa family/parental burden score
Authors: H. Dufresne, S. Hadj-Rabia, C. Taieb, C. Bodemer
Journal: British Journal of Dermatology (2015)

Journal Article Development and validation of an epidermolysis bullosa family/parental burden score Get access H. Dufresne, H. Dufresne Department of Dermatology Reference Center for Genodermatoses and Rare Skin Diseases (MAGEC) Institut Imagine Hôpital Universitaire Necker‐Enfants Malades 149 Rue de Sèvres 75015 Paris France Correspondence Hélène Dufresne. E‐mail: helene.dufresne@nck.aphp.fr Search for other works by this author on: Oxford Academic Google Scholar S. Hadj‐Rabia, S. Hadj‐Rabia Department of Dermatology Reference Center for Genodermatoses and Rare Skin Diseases (MAGEC) Institut Imagine Hôpital Universitaire Necker‐Enfants Malades 149 Rue de Sèvres 75015 Paris FranceNSERM U1163, Université Paris Descartes ‐ Sorbonne Paris Cité, Institut Imagine 149 Rue de Sèvres 75015 Paris France Search for other works by this author on: Oxford Academic Google Scholar C. Taieb, C. Taieb Public Health and Quality of Life Pierre Fabre Paris France Search for other works by this author on: Oxford Academic Google Scholar C. Bodemer C. Bodemer Department of Dermatology Reference Center for Genodermatoses and Rare Skin Diseases (MAGEC) Institut Imagine Hôpital Universitaire Necker‐Enfants Malades 149 Rue de Sèvres 75015 Paris FranceNSERM U1163, Université Paris Descartes ‐ Sorbonne Paris Cité, Institut Imagine 149 Rue de Sèvres 75015 Paris France Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 173, Issue 6, 1 December 2015, Pages 1405–1410, https://doi.org/10.1111/bjd.14072 Published: 01 December 2015
Health care professionals's features of an ideal inhaler in testing new inhaler device. Does it recognize their needs?
Authors: Tadeusz Plusa, Pawel Bijos
Journal: European Respiratory Journal (2014)

Backgrounds: In “classic” Dry Powder Inhalers, different priming procedures are used to release a measured dose of powder. The priming is a potential source of error. The aim of the study was to obtain opinions on the new inhaler [Spiromax] in doctors and nurses. Material and methods: It was conducted semi-quantitative market research with qualitative face to face interviews (each 75 minutes). HCPs tried out the device – questionnaires and noted down their observations. There were 181 HCP interviews in 9 European countries. Results: HCPs viewed of what the ideal inhaler should provide to meet their needs: (top 3 mentions)easy handling; confirmation of successful inhalation; convenient, ergonomic size and shape.  89% of HCPs using the current device [Symbicort Turbohaler] find the new device [Spiromax] more intuitive.  Three in four HCPs stated that the new device comes close(r) to their perception of an ideal inhaler. Conclusions. In HCPs opinion, the new device [Spiromax] was viewed to be preferred device than currently used inhaler and has characteristics that come close(r) to the ideal inhaler.
A Comparison of Intranasal and Oral Flunisolide in the Therapy of Allergic Rhinitis
Authors: A. Kwaselow, J. McLean, W. Busse, R. Bush, C. Reed, W. Metzger, H. Richerson, D. Shulan, J. Koshiver, M. Chaplin
Journal: Allergy (1985)

Intranasal flunisolide is an effective treatment for allergic rhinitis. Flunisolide has high bioavailability when administered to normal subjects (50 % of an intranasal dose reaches the systemic circulation) with minimal systemic effects. Bioavailability in patients with active rhinitis averages 62.4 ± 15.7%. The oral dose bioequivalent to 100 μg intranasally is 500 μg. To define the comparative trial and systemic effects of intranasal flunisolide in patients with active allergic rhinitis, a multicenter, randomized, double‐blind, placebo‐controlled study was conducted during the 1983 ragweed hayfever season. Ninety‐nine patients with ragweed hayfever for ≥2 years and positive prick skin tests to ragweed were randomly allocated to one of three treatment groups: 0 = oral flunisolide 500 μg b.i.d. and intranasal placebo b.i.d.; N = intranasal flunisolide 50 μg per nostril b.i.d. and oral placebo b.i.d.; P = intranasal and oral placebo b.i.d. Treatment continued for 4 weeks. Patients kept daily symptom scores. Patients were evaluated by a blinded observer every 2 weeks and were globally evaluated at the study's end. Data were analyzed for each center and pooled. There were no significant differences in symptom severity of sneezing, nasal congestion, and throat itch in the 0 (oral flunisolide) and P (placebo) groups. N (nasal flunisolide) was significantly more effective than 0 or P (P ≤= 0.005) for each symptom for at least one 2‐week period. Global evaluation demonstrated control of overall hayfever severity for N (nasal flunisolide) but not for 0 (oral flunisolide). We conclude that the therapeutic efficacy of flunisolide is achieved by topical and not by systemic action.
Appropriate level of volume targeting for ventilated infants born at or near term
Authors: Olie Chowdhury, Silke Lee, Simon Hannam, Gerrard F. Rafferty, Anne Greenough
Journal: European Respiratory Journal (2011)

Aims:  To determine the most appropriate level of volume targeting (VT) for ventilated infants born at or near term.   Methods:  Study one: expiratory tidal volumes (VTe) were measured during time cycled, pressure limited ventilation either using a pneumotachograph or the ventilator9s software; at least 80 breaths were analysed per infant. Study two: infants were studied at VT levels of 4, 5 and 6 mls/kg delivered in random order, with return to baseline between each VT level. The transdiaphragmatic pressure-time product (PTPdi), a measure of the work of breathing, was assessed at each VT level and during the baseline periods. To measure PTPdi, oesophageal and gastric pressures are measured and PTPdi calculated by integration of the transdiaphragmatic pressure signal with time for each breath and expressed per minute. In both studies, infants are only assessed when their blood gases are within the normal range.   Results:  To date: Study one: 20 infants, median gestational age of 39 (range 35-41) weeks, had a median VTe of 5 (range 1.4-9.7) mls/kg; 50% of infants had a VTe outside the “normal” tidal volume range (4 to 6mls/kg). Study two: the median PTPdi of 11 infants, median gestational age 39 (35-41) weeks, at a VT level of 4 mls/kg (310, IQR 244-332 cmH2O.s/min) was almost double that at 6 mls/kg (median 163, IQR 122-220 cmH2O.s/min), (p   Conclusion:  During time cycled, pressure limited ventilation, infants born at or near term are frequently ventilated using volumes outside the “normal” tidal range. Yet, even within that range, low compared to high levels of volume targeting significantly increase the work of breathing.
Application of a new ventilator-multi-plethysmograph system for testing efficacy of surfactant replacement in newborn rabbits
Authors: B Sun, T Kobayashi, T Cursted, G Grossmann, B Robertson
Journal: European Respiratory Journal (1991)

We applied a new ventilator-multi-plethysmograph system to evaluate the effect of surfactant replacement in newborn rabbits under well controlled, nearly physiological conditions characterized by normal ECG and adequate PCO2 in right ventricular heart blood obtained at the end of the experiment. Up to 10 animals were ventilated in parallel with a pressure-constant common respirator system. Using a working pressure of 4.9 kPa (50 cmH2O), we could adjust the pressure delivered to each animal within the range of 0.49-4.4 kPa (5-45 cmH2O), by changing the length of an open high-resistance tube constituting the outflow limb of the connection between the common ventilator tube and the tracheal cannula. Immature newborn animals obtained after 27.5 days gestation and ventilated for 30 min with a tidal volume of 8-10 ml.kg-1 had a mean +/- SD lung-thorax compliance of 4.2 +/- 1.1 ml.kPa-1.kg-1 (0.41 +/- 0.11 ml.cmH2O-1.kg-1) and PCO2 of 8.5 +/- 1.9 kPa. In littermates treated at birth with a large dose of natural surfactant (Curosurf, 200 mg.kg-1, compliance increased to 6.0 +/- 1.0 ml.kPa-1.kg-1 (0.68 +/- 0.10 ml.cmH2O-1.kg-1) (p less than 0.01) and PCO2 decreased to 6.9 +/- 1.2 kPa (p less than 0.01). Near-term animals, obtained at 30 days gestation and ventilated under similar conditions had a compliance of 7.2 +/- 0.9 ml.kPa-1.kg-1 (0.71 +/- 0.09 ml.cmH2O-1.kg-1) and PCO2 of 6.4 (1.2) kPa. Administration of surfactant (same dose as above) to these mature animals at birth had no adverse effects.
Late-breaking abstract: Comparison of bone marrow derived-mononuclear cells with mesenchymal stem cells on inflammatory and remodeling processes in experimental chronic allergic asthma
Authors: Soraia Abreu, Mariana Antunes, Debora Xisto, Milena Vasconcellos, Julia Crossetti, Vera Capelozzi, Marcelo Morales, Patricia Rocco
Journal: European Respiratory Journal (2011)

Recently, we demonstrated the beneficial effects of bone marrow-derived mononuclear cells (BMDMCs) on lung morphofunction in experimental chronic allergic asthma. Mesenchymal stem cells (MSCs) have the potential to serve as a universal source for replacement of specific cells in several diseases and thus may offer hope as a potential therapeutic intervention for the treatment of the chronic remodeling changes that occur in asthma. This study aimed to compare the therapeutic effects of BMDMCs with MSCs on inflammatory and remodeling processes in experimental chronic allergic asthma. Thirty-six C57BL/6 mice were assigned to two groups. In the OVA group, mice were sensitized and repeatedly challenged with ovalbumin. Control mice (C) received saline under the same protocol. C and OVA groups were further randomized to receive saline (SAL), BMDMCs or MSCs (1×10 5 ) intratracheally 24 h after the last challenge. After one week, airway resistance, viscoelastic pressure, static elastance, as well as the degree of lung inflammation and remodeling (light microscopy and immunohistochemistry) were analyzed. BMDMC and MSC therapies led to a reduction in eosinophil infiltration and fibrosis in airway and lung parenchyma compared to OVA leading to a reduction in airway resistance and viscoelastic pressure. These parameters were more reduced after BMDMCs compared to MSCs. In conclusion, in the present model of chronic allergic asthma, both BMDMC and MSC therapies were effective at modulating the inflammatory and fibrogenic processes, however, BMDMC administration led to greater beneficial effect.  Supported by: PRONEX, CNPq, FAPERJ, CAPES.