THE URINARY EXCRETION TEST IN THE DIAGNOSIS OF ADDISONIAN PERNICIOUS ANEMIA
Authors: S F RABINER, H C LICHTMAN, J MESSITE, R J WATSON, V GINSBERG, L ELLENBOGEN, W L WILLIAMS
Journal: Annals of Internal Medicine (1956)

Article1 March 1956THE URINARY EXCRETION TEST IN THE DIAGNOSIS OF ADDISONIAN PERNICIOUS ANEMIAS. FRED RABINER, M.D., HERBERT C. LICHTMAN, M.D., JACQUELINE MESSITE, M.D., R. JANET WATSON, M.D., F.A.C.P., VICTOR GINSBERG, M.D., F.A.C.P., LEON ELLENBOGEN, Ph.D., WILLIAM L. WILLIAMS, Ph.D.S. FRED RABINER, M.D.Search for more papers by this author, HERBERT C. LICHTMAN, M.D.Search for more papers by this author, JACQUELINE MESSITE, M.D.Search for more papers by this author, R. JANET WATSON, M.D., F.A.C.P.Search for more papers by this author, VICTOR GINSBERG, M.D., F.A.C.P.Search for more papers by this author, LEON ELLENBOGEN, Ph.D.Search for more papers by this author, WILLIAM L. WILLIAMS, Ph.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-44-3-437 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptAddisonian pernicious anemia is a deficiency disease resulting from the lack of a specific gastric secretion which is essential for the optimal absorption in the intestine of orally ingested vitamin B12. Probably a mucoprotein,1, 2 this gastric enzyme, commonly referred to as intrinsic factor,3, 4 is produced by the mucosal cells of the stomach of man and other animals. It has not yet been fully characterized chemically, nor is there available a direct method for detecting its presence in the stomach secretions.In hematologic relapse the diagnosis of pernicious anemia is readily established by the findings of megaloblastic erythropoiesis, macrocytic...Bibliography1. WilliamsEllenbogenEsposito WWLRG: Preparation of highly purified intrinsic factor, Proc. Soc. Exper. Biol. and Med. 7: 400, 1954. CrossrefGoogle Scholar2. GlassBoydRubinsteinSvigals GBLJMACS: Relationship of glandular mucoprotein from human gastric juice to Castle's intrinsic antianemic factor, Science 115: 101, 1952. CrossrefMedlineGoogle Scholar3. CastleTownsend WBWC: Observations on etiologic relationship of achylia gastrica to pernicious anemia. II. Effect of administration to patients with pernicious anemia of beef muscle after incubation with normal human gastric juice, Am. J. M. Sc. 178: 764, 1929. CrossrefGoogle Scholar4. CastleTownsendHeath WBWCCW: Observations on etiologic relationship of achylia gastrica to pernicious anemia. III. Nature of reaction between normal human gastric juice and beef muscle leading to clinical improvement and increased blood formation similar to effect of liver feeding, Am. J. M. Sc. 180: 305, 1930. CrossrefGoogle Scholar5. Schilling RF: Intrinsic factor studies. II. The effect of gastric juice on the urinary excretion of radioactivity after oral administration of vitamin B12 Co60 , J. Lab. and Clin. Med. 42: 860, 1953. MedlineGoogle Scholar6. RabinerEllenbogenLichtmanWilliamsKabakowWatson SFLHCWLBRJ: Urinary excretion of vitamin B12 Co60 as a method of assay for intrinsic factor, Clin. Res. Proc. 3: 28, 1955. Google Scholar7. EllenbogenWilliamsRabinerLichtman LWLSFHC: An improved urinary excretion test as an assay for intrinsic factor, Proc. Soc. Exper. Biol. and Med. 89: 357, 1955. CrossrefMedlineGoogle Scholar8. GlassBoydGellinStephanson GBLJGAL: Uptake of radioactive vitamin B12 by the liver in humans: test for measurement of intestinal absorption of vitamin B12 and intrinsic factor activity, Arch. Biochem. and Biophys. 51: 251, 1954. CrossrefMedlineGoogle Scholar9. HeinleWelchScharfMeachamPrusoff RWADVGCWM: Studies of excretion (and absorption) of Co60 labelled B12 in pernicious anemia, Tr. A. Am. Physicians 65: 214, 1952. MedlineGoogle Scholar10. CallanderTurnbullWakisaka STAG: Estimation of intrinsic factor by the use of radioactive B12 , Brit. M. J. 1: 10, 1954. CrossrefMedlineGoogle Scholar11. Meyer L: Oral administration of Co60 B12 in pernicious anemia, Proc. Soc. Exper. Biol. and Med. 82: 490, 1953. CrossrefMedlineGoogle Scholar12. Castle WB: Development of knowledge concerning the gastric intrinsic factor and its relation to pernicious anemia, New England J. Med. 249: 603, 1953. CrossrefMedlineGoogle Scholar13. WallersteinHarrisSchillingCastle ROJWRFWB: Observations on etiologic relationship of achylia gastrica to pernicious anemia. XV. Hematopoietic effects of simultaneous intravenous and of simultaneous or serial oral administrations of intrinsic factor and vitamin B12 , J. Lab. and Clin. Med. 41: 363, 1953. MedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Pearl River, New York*Received for publication August 8, 1955.From the Division of Hematology, Department of Medicine, State University of New York College of Medicine at New York City, Brooklyn, N. Y., Kings County Hospital, and the Nutrition and Physiology Section of American Cyanamid Company, Research Division, Lederle Laboratories, Pearl River, N. Y. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byUse of radioisotope techniques in the clinical evaluation of patients with megaloblastic anemiaVitamin B 12 Malabsorption in Chronic Pancreatic InsufficiencyIntrinsic FactorVitamin B12 and Intrinsic FactorRadio-Vitamin B12 in der klinischen DiagnostikUntersuchungen mit radioaktivem Vitamin B12Effect of Renal Disease on the Schilling Test 1 March 1956Volume 44, Issue 3Page: 437-445KeywordsExcretionHematology and oncologyHospital medicineMegaloblastic anemiaNutritionOral administrationPernicious anemiaResearch laboratoriesRiversStomach ePublished: 1 December 2008 Issue Published: 1 March 1956 Copyright & PermissionsCopyright ©, 1956, by The American College of PhysiciansPDF downloadLoading ...