tbuckley/cabot-search huggingface dataset:
588. A Central Role of RNA in Developing Therapeutic Vaccines for Cancer and Infectious Diseases
Authors: Shulin Li, Francesca Mellieon, Robin Barret
Journal: Molecular Therapy (2004)

Intratumoral (tumor local) and intramuscular (systemic) immuno-electrogenetherapies are important for treating solid tumor and residual or metastatic tumors, respectively. However, intratumoral injection of IL-12 gene via electroporation is more effective in the inhibition of tumor growth than intramuscular injection, but the underlying mechanism that causes this administration route-dependent inhibition of tumor growth is not fully understood. To understand the cellular mechanism by which tumor growth is significantly inhibited by intratumoral but not by intramuscular IL-12 electro-gene therapies, induction of T-cell infiltration, CTL activity, inhibition of angiogenesis and transgene expression were determined using northern blot, ELISA, immunohistochemistry, and fluorescence-based CTL analysis, and the therapeutic effect was determined in immunocompetent, immune cell-depleted, and immunodeficient mice. The results indicated that intratumoral electric pulses, when combined with the injection of plasmid DNA, is one of the primary factors that results in the different therapeutic efficacies between intratumoral and intramuscular delivery. Intratumoral injection of control plasmid DNA via electric pulses yields a small tumor volume that is only 37% and 58% of the tumor volumes from mice treated with intramuscular injection of plasmid DNA without and with IL-12 via electroporation, respectively. Inclusion of IL-12 in the intratumoral injection further inhibits the tumor growth and yields a tumor volume that is 6% and 9% of the tumor volume from mice treated with intramuscular injection of control plasmid DNA and IL-12 gene, respectively. CD8 T-cells are the primary effector cells that are associated with this administration route-dependent tumor inhibition because intratumoral, but not intramuscular injection via electric pulses induces CD8+ T-cell infiltration and CTL activity, and because both intratumoral and intramuscular IL-12 electro-gene therapies induce similar levels of antiangiogenesis-and NK cell-mediated antitumor effect. These findings indicate that induction of tumor-specific CD8+ T-cell response is a prerequisite for improving the therapeutic effect mediated by the systemic immuno-electrogenetherapy.
Abstract 16763: Mechanical Circulatory Support Promotes Myocardial Nitric Oxide Signaling in Heart Failure Patients
Authors: David J Polhemus, Ya-Xiong Tao, Steven C Koenig, Sumanth D Prabhu, David Lefer
Journal: Circulation (2014)

Introduction: The integrity of the cardiovascular system is dependent on the continuous generation of nitric oxide (NO). Reduction in NO bioavailability is central to the development of cardiovascular disorders and patients with heart failure (HF) exhibit blunted endothelium-dependent flow-mediated dilation in indicating diminished NO release. We have previously reported that patients with heart failure have marked reductions in circulating NO metabolites and that NO-based therapies significantly reverse left ventricular (LV) dysfunction in preclinical animal models of HF. Methods: Plasma and left ventricular (LV) tissue were collected in HF patients (n=10) at the time of left ventricular assist device (LVAD) implantation. Plasma and LV tissue were again collected in the same 10 patients at the time of cardiac transplantation. Plasma and cardiac nitrite concentrations were determined by ion chromatography methods and nitrosylated protein (RXNO) levels were measured by chemiluminescence detection. Cyclic guanosine 5’-monophosphate (cGMP) was analyzed using radioimmunoassay technique. Results: Results: Following LVAD implantation, RXNO (NO-bound protein) levels were significantly elevated in both circulation and in the LV (10.2 nM vs. 33.3 nM, p &lt; 0.05). Downstream NO signaling activates soluble guanylyl cyclase to form cGMP. Interestingly, LV cGMP levels were significantly augmented post-LVAD implantation (p&lt;0.01). In contrast, plasma and cardiac nitrite (NO2) levels remained unchanged. Conclusions: These data strongly suggest that cardiovascular remodeling accompanying LVAD implantation promotes endothelial nitric oxide synthase (eNOS) function and increases NO bioavailability and signaling.
Potential Adverse Effects of Anesthesia in Children
Authors: Christopher M. Ward, Kirk J. Hogan, Roderic G. Eckenhoff
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 19678: Safety of Cryoablation of Atrial Fibrillation in the Presence of Mechanical Mitral Valve
Authors: Srinivas Rajsheker, Pradeep Gujja, Mehran Attari
Journal: Circulation (2014)

Introduction: Pulmonary vein isolation is an effective therapy for recurrent, symptomatic, drug-refractory atrial fibrillation (AF). Radiofrequency ablation (RFA) has long been the standard of care, while cryoballoon technology has emerged as a feasible approach with promising results in paroxysmal AF. There is risk of catheter entrapment during RFA in patients with mechanical mitral valve and the experience is limited. To the best of our knowledge pulmonary vein isolation (PVI) with cryoablation has never been reported in a patient with a mechanical mitral valve. Case: A 52 year old male presented with symptomatic paroxysmal AF, nonischemic cardiomyopathy biventricular defibrillator, mechanical aortic and mitral valve replacement for rheumatic valvular disease. The patient was highly symptomatic while on sotalol therapy. We proceeded with PVI using cryoablation technology. Procedure: After a single transseptal puncture, a 28 mm cryoballoon catheter and 20mm 8 pole circular recording catheter were advanced into the left atrium. All four pulmonary veins were isolated with the aid of intracardiac echo, fluoroscopy and Ensite 3-D mapping. Caution was maintained to minimize catheter manipulation and to keep the catheters away from the mechanical valvular plane. Discussion: The use of cryoablation for PVI has several potential advantages over radiofrequency ablation including greater improvement in fluoroscopic time and total procedure duration. The risk of prosthetic valve dysfunction due to trauma from ablation catheter, and entrapment of the circular mapping catheter in the mitral valve apparatus represent major concerns when performing PVI in patients with mitral valve replacement, however, less need for manipulation of catheter is an advantage of cryoablation compared to RFA in this case. This case illustrates that cryoablation can be successfully performed in a patient with a prosthetic mechanical mitral valve.
Initiation of antiretroviral therapy during primary HIV-1 infection induces rapid stabilization of the T-cell receptor β chain repertoire and reduces the level of T-cell oligoclonality
Authors: Hugo Soudeyns, Gabriele Campi, G. Paolo Rizzardi, Caterina Lenge, James F. Demarest, Giuseppe Tambussi, Adriano Lazzarin, Daniel Kaufmann, Giulia Casorati, Lawrence Corey, Giuseppe Pantaleo
Journal: Blood (2000)
Dual treatment with atazanavir–ritonavir plus lamivudine versus triple treatment with atazanavir–ritonavir plus two nucleos(t)ides in virologically stable patients with HIV-1 (SALT): 48 week results from a randomised, open-label, non-inferiority trial
Authors: José A Perez-Molina, Rafael Rubio, Antonio Rivero, Juan Pasquau, Ignacio Suárez-Lozano, Melcior Riera, Miriam Estébanez, Jesús Santos, José Sanz-Moreno, Jesús Troya, Ana Mariño, Antonio Antela, Jordi Navarro, Herminia Esteban, Santiago Moreno
Journal: The Lancet Infectious Diseases (2015)
Current Dermatologic Management.
Authors: Walter R. Nickel
Journal: Annals of Internal Medicine (1971)

is quite familiar with, having tasted it many times during his lifetime?Why should he fear what is apparently a permanent condition and state of unifica- tion?The point of this book is that what we think we dread above all else is really what we most desire."We do not know what death is-certainly not in the sense of sentient beings who can-experience it, for certainly a fundamental and keystone difference between the exam- ples of unification described and death is that the exam- ples cited, particularly orgasm, are experiences, albeit in- effable; whereas, there is nothing to lead one to conclude that death is an etemal sentient experience.The rest of the points falter around this important distinction.
Abstract 15781: High Dose Guideline-Directed Medical Therapy for Heart Failure at Discharge Improves 1-Year Mortality in African-Americans but Not Hypertensive Patients
Authors: Khadijah Breathett, Rod Sturdivant, Daniel Carpenter, Randi Foraker, Phillip Binkley, William T Abraham
Journal: Circulation (2014)

BACKGROUND: Guideline-directed medical therapy (GDMT) for chronic heart failure has resulted in improved outcomes in readmission and mortality; yet, the effect of achieving high doses (>50% target) GDMT at discharge is unknown. We hypothesized that patients discharged at >50% target GDMT compared to ≤50% target GDMT would have reduced readmission and mortality. METHODS: We retrospectively evaluated 1408 patients who presented with a heart failure admission and were discharged alive between October 2011 and October 2012 at our academic (Aca) and academic-community hybrid (Hybrid) medical centers. Logistic regression and multinomial logit models were used to assess for 90-day and 1-year readmission and mortality based upon GDMT dosing including ace inhibitors, angiotensin receptor blockers, beta blockers, and aldosterone receptor blockers. RESULTS: Patients differed between the two categories by race [Aca 233 (21.3%) African-Americans (AA) versus Hybrid 227 (72.5%) AA, p<0.001] and systolic blood pressure (...
Mucous Colitis (Colonic Vagotonia)
Authors: John Dudley Dunham
Journal: Annals of Internal Medicine (1927)

Article1 July 1927Mucous Colitis (Colonic Vagotonia)JOHN DUDLEY DUNHAM, A.B., M.D.JOHN DUDLEY DUNHAM, A.B., M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-1-1-9 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe synonyms by which this disease is known are numerous and confusing. Writers have used the terms: Myxorrhea coli, muco-membranous colitis, myxoneurosis intestinalis membranacea, hypochondriasis pituitosa, catarrhal colitis, and chronic intestinal catarrh, most of which are misnomers.The few necropsies (1) performed on patients dead of this disease when uncomplicated reveal no evidence of an inflammation of the large bowel.Hence, colitis conveys a very erroneous conception. Cabot's (2) suggestion "colica mucosa" is better, though the part affected is not mentioned.Colonic vagotonia covers the part involved as well as the etiologic factor. The first description of this disease has...Bibliography(1) SOMMERVILLE D: International Clinics, vol. i, 1908, p. 77. Google Scholar(2) CABOT R: Jour. A. M. A., September 27, 1913, vol. lxi, No. 13. CrossrefGoogle Scholar(3) Psalms 90-10. Google Scholar(4) NORMAN NP: Am. Jour. Electrotherapeutics and Radiology, 1923, xli, 120. Google Scholar(5) GOLDMAN C: Myxomembranous Colitis, Med. Jour. & Record, Dec. 16, 1925. Google Scholar(6) DA COSTA JM: Membranous Enteritis, Am. Jour. Med. Sciences, October, 1871. CrossrefGoogle Scholar(7) MIX CL: Mucous Colitis, Medical Clinics of N. America, Sept., 1924. Google Scholar(8) SPITZIG BL: Vagotony and Its Relation to Mucous Colitis, Jour. A. M. A. vol. lxii, No. 5, p. 364. CrossrefGoogle Scholar(9) LUGINBUHL CB: Chronic Colitis, Iowa State Med. Jour., xii, 96-100. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Columbus, Ohio*Read at the Eleventh Annual Clinical Session of The American College of Physicians, Cleveland, Ohio, February 21, 1927. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 July 1927Volume 1, Issue 1Page: 9-12KeywordsColitisInflammationInflammatory bowel diseaseIrritable bowel syndromeMucous membranes ePublished: 1 December 2008 Issue Published: 1 July 1927 PDF downloadLoading ...
Functional investigation of tumor suppressive role of chromosome 9 in esophageal squamous cell carcinoma
Authors: L. C. Yang, J. C. O. Tang, G. Srivastava, E. J. Stanbridge, M. L. Lung
Journal: Journal of Clinical Oncology (2004)

9571 Background: Esophageal carcinoma (EC) is a very deadly disease, but its molecular basis for tumorigenesis is still largely unknown. The microcell chromosome transfer technique is useful to functionally validate the presence of tumor suppressor genes (TSGs). Early studies indicate there might be important TSGs for EC on chromosome 9. Our study found the LOH frequency of chromosome 9 was very high and three commonly deleted regions were observed at 9p23–22, 9q13–22.3, and 9q34, which suggest the probable locations of TSGs on chromosome 9 involved in esophageal tumorigenesis. Methods: Transfer of chromosome 9 into the highly tumorigenic EC cell line, SLMT-1S1, was accomplished by the method of microcell-mediated chromosome transfer (MMCT). Hybrid cells and their corresponding tumor segregants were studied for the ability to form tumors in a nude mouse model and 33 microsatellite markers were used to detect critical regions associated with their tumor suppression. Results: Comparison of the tumor suppressive hybrid cell lines and their paired tumor segregants, showed some critical regions were non-randomly eliminated, including the 9q22.1 and 9q22.3 regions. Conclusions: These results indicate several chromosome 9q regions may contain important TSGs for EC. These functional complementation experiments are expected to map the putative TSGs of EC. No significant financial relationships to disclose.
Pathogenesis, prediction and trials for the prevention of insulin-dependent (type 1) diabetes mellitus1Abbreviations: s.c., subcutaneous; i.p., intraperitoneal; i.v., intravenous; IFA, incomplete Freund's adjuvant; DTP, diphtheria-tetanus-pertussis; LCMV, lymphocytic choriomeningitis virus; GAD, glutamic acid decarboxylase; ICA, islet cell autoantibody; JDF, juvenile diabetes foundation; IAA, insulin autoantibody; GAA, glutamic acid decarboxylase autoantibody; DM, diabetes mellitus; RIA, …
Authors: Patricia M. Graves, George S. Eisenbarth
Journal: Advanced Drug Delivery Reviews (1999)
430. AAV-Microbead Conjugates for the Enhanced Delivery of AAV-Based Vectors
Authors: Yuanqing Lu, Mei Tang, Clive Wasserfall, Young-Kook Choi, Thomas Gardmann, Martha Campbell-Thompson, Mark A. Atkinson, Sihong Song
Journal: Molecular Therapy (2004)

Although the cause of type 1 diabetes is complex and multifactorial, immunoregulatory and anti-inflammatory strategies hold great potential for preventing the development of this autoimmune disease. Alpha 1 antitrypsin and elafin are serine proteinase inhibitors (serpin) having potent anti-inflammatory properties. We have previously shown that recombinant adeno-associated virus 2 (rAAV2) mediated human alpha 1 antitrypsin (hAAT) delivery to skeletal muscle resulted in a 70% reduction of type 1 diabetes in female NOD mice. However, serum levels of hAAT were undetectable. In the present study, we sought to achieve more an effective means of prevention with rAAV serotype 1 vectors carrying hAAT or elafin genes. Cohorts of 8 week old female NOD mice (n=10) were intramuscularly injected with rAAV1-CB-AAT (2 × 1011 particles/mouse), rAAV1-CB-elafin (2 × 1011 particles/mouse) or saline. In hAAT animals, serum hAAT levels were sustained (300ug/ml) for at least 26 weeks, despite the development of antibodies against hAAT. Serum elafin levels were somewhat lower in elafin animals, averaging 10ng/ml. Importantly, at 32 weeks of age, only 10% of rAAV1-CB-AAT treated mice developed diabetes versus 80% of rAAV1-CB-elafin and 70% of saline (P<0.001). In order to understand the mechanism of this protective effect, we performed adoptive transfer experiments. When 2 × 106 splenocytes from rAAV-CB-AAT treated mice (diabetes free) were IP injected into NOD-Scid mice, 60% (5/8) recipients remained diabetes free for 12 weeks post injection. In contrast, when splenocytes from newly diabetic mice were injected, 100% of recipient developed diabetes within 5 weeks. Spectratyping of T cell receptors showed that gene delivery altered the Vb repertoires in splenocytes. Results from this study showed that alpha 1 antitrypsin gene therapy was effective in NOD mouse model and suggest that anti-inflammatory protease inhibitors might represent a novel a clinical application for preventing type 1 diabetes.
Withdrawn
Authors: Frank Q. Nuttall, Mary C. Gannon, Heidi Hoover
Journal: Diabetes Care (2011)

Our research group has developed diets we refer to as Low Biologically Available Glucose (LoBAG) diets. These diets, in short-term studies (5–10 weeks), result in a decrease in glycated hemoglobin that is similar to or greater than that typically obtained with oral medications in patients with type 2 diabetes (1–3). The diet is lower in carbohydrates (CHOs) and higher in protein and fat than that recommended by the American Heart Association (4) and the U.S. Department of Agriculture (5). It is not ketogenic and does not require weight loss. The diets are designated by the CHO content, which is given as a subscript; for example, if the CHO content is 30% of food energy, the diet would be termed as a LoBAG30 diet.
AIDS: the global challenge
Authors: Tessa Richards
Journal: BMJ (2001)

With 23 million people dead and 36 million HIV positive, the need to do more to tackle AIDS is painfully clear. This week's BMJ (pp 1440, 1451, 1475) draws attention to the catastrophe. To get a comprehensive internet update on AIDS, the best website to visit is www.aegis.com. This is the largest HIV/AIDS site and it …
Anticancer effect of tectochrysin in colon cancer cell via suppression of NF-kappaB activity and enhancement of death receptor expression
Authors: Mi Hee Park, Ji Eun Hong, Eun Sook Park, Hee Sung Yoon, Doo Won Seo, Byung Kook Hyun, Sang-Bae Han, Young Won Ham, Bang Yeon Hwang, Jin Tae Hong
Journal: Molecular Cancer (2015)

Flavonoids are a diverse family of natural phenolic compounds commonly found in fruits and vegetables. Epidemiologic studies showed that flavonoids also reduce the risk of colon cancer. Tectochrysin is one of the major flavonoids of Alpinia oxyphylla Miquel. However, the anti-cancer effects and the molecular mechanisms of tectochrysin in colon cancer cells have not yet been reported. We investigated whether tectochrysin could inhibit colon cancer cell growth at 1, 5, 10 μg/ml. In in vivo study, we injected a tectochrysin treatment dose of 5 mg/kg to each mouse. Tectochrysin suppressed the growth of SW480 and HCT116 human colon cancer cells. The expression of DR3, DR4 and Fas were significantly increased, and pro-apoptotic proteins were also increased. Tectochrysin treatment also inhibited activity of NF-κB. A docking model indicated that tectochrysin binds directly to the p50 unit. In in vivo, tumor weights and volumes in mice were reduced when treated with tectochrysin. Tectochrysin leads to apoptotic cell death in colon cancer cells through activation of death receptors expression via the inhibition of NF-κB. Tectochrysin can be a useful agent for the treatment of colon cancer cell growth as well as an adjuvant agent for chemo-resistant cancer cells growth.
Outcome Depend On Age But Not Comorbidity Score, In Patients treated With Fludarabine and Busulfan (FB4) As Conditioning Regimen In Allogeneic Stem Cell Transplantation
Authors: Javier Nunez, Arancha Bermudez, Lucrecia Yanez, Guillermo Martin, Andres Insunza, Carlos Richard, Carmen Montes Gaisan, Eulogio Conde
Journal: Blood (2013)
Abstract 18092: Nitroxyl (HNO) Improves Ventricular Relaxation and Ca2+-Handling in Rats with Induced Chronic Diastolic Dysfunction
Authors: Steve R Roof, Craig Hartman, John Reardon, Carlos del Rio, Mark Ziolo, Robert Hamlin
Journal: Circulation (2014)

Despite the morbidity and clinical burden of primary diastolic cardiac dysfunction, effective therapies for patients presenting with heart failure with preserved ejection fraction (HFpEF) are limited. Nitroxyl (HNO) produces cAMP-independent functional support, in part by improving myocardial calcium-handling. We hypothesize that HNO can rescue lusitropic function when acutely administered to rats with chronic diastolic dysfunction, mimicking HFpEF. Diastolic dysfunction, as demonstrated by decreased E/A ratios via echocardiography, was induced by either 4 weeks of chronic isoproterenol administration (via a mini-osmotic pump; ISO) or by renoprival hypertension (secondary to renal wrapping; RW) for 5 weeks. In vivo left-ventricular hemodynamics/pressure-volume relationships were assessed before/during a 30 minute IV administration of a HNO pro-drug (CXL-1020, 100 μg/kg/min). Ca2+ handling in isolated cardiomyocytes was also studied (CXL-1020, at 50 μM). In both cases, milrinone (mil) was used as a cAMP-dependent positive control (at a matched-efficacy in healthy rats, i.e., 10 μg/kg/min and 0.5 μM) In the setting of chronic diastolic dysfunction, HNO lowered the LV filling pressures in both models by a combined average of 46 ± 8% versus baseline, accelerated the time-constant (tau) of relaxation (-26 ± 3%) and improved compliance (flattened the end-diastolic PV relationship) (-61 ±3%). In myocytes isolated from dysfunctional hearts, HNO accelerated the time for Ca2+ to decline to 50% of its max amplitude (RT50) by -10 ± 2% and myocyte relengthening by -12 ± 3% in ISO rats. Despite eliciting comparable responses in healthy animals/myocytes, the HNO-mediated effects on both tau (in vivo) (-33 ± 3% vs mil -25 ± 2%) and Ca2+ decline (myocyte) (-10 ± 2% vs mil -6 ± 1%) were greater than those of milrinone in the ISO rats, which had greater impairment at baseline compared to RW rats. In conclusion, acute-therapy with HNO improved ventricular relaxation, compliance and Ca2+ handling, in the setting of chronic diastolic dysfunction. Moreover, these effects were greater than observed with the cAMP-dependent agent milrinone.