tbuckley/cabot-search huggingface dataset:
REG1Aand its receptor EXTL3 are prognostic markers in colorectal cancer
Authors: Wolfgang Kemmner, Christian Astrosini
Journal: Clinical Cancer Research (2006)

PR-7   Purpose: By microarray screening for genes with prognostic relevance in colorectal cancer, we found  Regenerating Islet-Derived 1 Alpha  ( REG1A ) to be upregulated in patients with an unfavorable clinical outcome. For validation,  REG1A  expression was quantified in a colorectal cancer patient cohort by Taqman PCR and localized by tissue microarray based in-situ hybridization. Simultaneously, expression of REG1A receptor  Exostoses (Multiple)-Like 3  ( EXTL3 ) was assessed.Experimental Design: Tumor and normal tissue from 63 nonpretreated colorectal cancer patients were analyzed with respect to  REG1A  expression by quantitative real-time PCR. Additionally ,  31 mucosa biopsies from healthy individuals and 22 adenomas were included. Tissue microarray was constructed with tumor and normal tissue samples from 42 colorectal cancer patients.  EXTL3  expression was determined in 56 individuals by Taqman PCR.Results:  REG1A  was significantly upregulated in tumor specimens, and in adenoma (p = 0.005) vs normal tissue (p  REG1A  turned out to be a significant predictor of disease free survival (p  insitu  hybridization it was verified to be confined to the crypt epithelium. Similarly,  EXTL3  displayed a tendency of upregulation in peritoneal carcinomatosis and could be successfully exploited for predicting patient outcome (p  REG1A  and its receptor  EXTL3  are markers of prognostic value in colorectal cancer. Furthermore, our data suggest that  REG1A  and  EXTL3  are associated with peritoneal carcinomatosis.
The M Protein of SARS-CoV: Basic Structural and Immunological Properties
Authors: Yongwu Hu, Jie Wen, Lin Tang, Haijun Zhang, Xiaowei Zhang, Yan Li, Jing Wang, Yujun Han, Guoqing Li, Jianping Shi, Xiangjun Tian, Feng Jiang, Xiaoqian Zhao, Jun Wang, Siqi Liu, Changqing Zeng, Jian Wang, Huanming Yang
Journal: Genomics Proteomics & Bioinformatics (2003)

Abstract We studied structural and immunological properties of the SARS-CoV M (membrane) protein, based on comparative analyses of sequence features, phylogenetic investigation, and experimental results. The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior. The M protein harbors a higher substitution rate (0.6% correlated to its size) among viral open reading frames (ORFs) from published data. The four substitutions detected in the M protein, which cause non-synonymous changes, can be classified into three types. One of them results in changes of pI (isoelectric point) and charge, affecting antigenicity. The second changes hydrophobicity of the TM region, and the third one relates to hydrophilicity of the interior structure. Phylogenetic tree building based on the variations of the M protein appears to support the non-human origin of SARS-CoV. To investigate its immunogenicity, we synthesized eight oligopeptides covering 69.2% of the entire ORF and screened them by using ELISA (enzyme-linked immunosorbent assay) with sera from SARS patients. The results confirmed our predictions on antigenic sites.
Mutant WD-repeat protein in triple-A syndrome
Authors: Anna Tullio-Pelet, Rémi Salomon, Smaïl Hadj-Rabia, Claude Mugnier, Marc-Henri de Laet, Beji Chaouachi, Fawzi Bakiri, Philippe Brottier, Laurence Cattolico, Clothilde Penet, Martine Bégeot, Danielle Naville, Marc Nicolino, Jean-Louis Chaussain, Jean Weissenbach, Arnold Munnich, Stanislas Lyonnet
Journal: Nature Genetics (2000)
Intensive Blood Glucose Control and Vascular Outcomes in Patients with Type 2 Diabetes
Authors: Anushka Patel, Stephen MacMahon, John Chalmers, Bruce Neal, Laurent Billot, Mark Woodward, Michel Marre, Mark Cooper, Paul Glasziou, Diederick Grobbee, Pavel Hamet, Stephen Harrap, Simon Heller, Lisheng Liu, Giuseppe Mancia, Carl Erik Mogensen, Changyu Pan, Neil Poulter, Anthony Rodgers, Bryan Williams, Severine Bompoint, Bastiaan E de Galan, Rohina Joshi, Florence Travert
Journal: New England Journal of Medicine (2008)

In patients with type 2 diabetes, the effects of intensive glucose control on vascular outcomes remain uncertain.We randomly assigned 11,140 patients with type 2 diabetes to undergo either standard glucose control or intensive glucose control, defined as the use of gliclazide (modified release) plus other drugs as required to achieve a glycated hemoglobin value of 6.5% or less. Primary end points were composites of major macrovascular events (death from cardiovascular causes, nonfatal myocardial infarction, or nonfatal stroke) and major microvascular events (new or worsening nephropathy or retinopathy), assessed both jointly and separately.After a median of 5 years of follow-up, the mean glycated hemoglobin level was lower in the intensive-control group (6.5%) than in the standard-control group (7.3%). Intensive control reduced the incidence of combined major macrovascular and microvascular events (18.1%, vs. 20.0% with standard control; hazard ratio, 0.90; 95% confidence interval [CI], 0.82 to 0.98; P=0.01), as well as that of major microvascular events (9.4% vs. 10.9%; hazard ratio, 0.86; 95% CI, 0.77 to 0.97; P=0.01), primarily because of a reduction in the incidence of nephropathy (4.1% vs. 5.2%; hazard ratio, 0.79; 95% CI, 0.66 to 0.93; P=0.006), with no significant effect on retinopathy (P=0.50). There were no significant effects of the type of glucose control on major macrovascular events (hazard ratio with intensive control, 0.94; 95% CI, 0.84 to 1.06; P=0.32), death from cardiovascular causes (hazard ratio with intensive control, 0.88; 95% CI, 0.74 to 1.04; P=0.12), or death from any cause (hazard ratio with intensive control, 0.93; 95% CI, 0.83 to 1.06; P=0.28). Severe hypoglycemia, although uncommon, was more common in the intensive-control group (2.7%, vs. 1.5% in the standard-control group; hazard ratio, 1.86; 95% CI, 1.42 to 2.40; P<0.001).A strategy of intensive glucose control, involving gliclazide (modified release) and other drugs as required, that lowered the glycated hemoglobin value to 6.5% yielded a 10% relative reduction in the combined outcome of major macrovascular and microvascular events, primarily as a consequence of a 21% relative reduction in nephropathy. (ClinicalTrials.gov number, NCT00145925.)
Autoradiographic Analysis of Cell Proliferation in Spontaneous Breast Cancer of C3H Mouse. II. Growth and Survival of Cells Labeled With Tritiated Thymidine&lt;xref ref-type="fn" rid="FN1"&gt;2&lt;/xref&gt;
Authors: Mortimer L. Mendelsohn
Journal: JNCI Journal of the National Cancer Institute (1960)

Autotransplanted breast tumors of C3H/Jax mice were removed at various periods up to 4 weeks after intravenous injection of 50 or 100 µc. of tritiated thymidine. Autoradiographs of the tumors were counted in detail and the following indexes were determined: Cells/Field, Mitoses/100 Cells, Degenerates/100 Cells, Labeled Cells/100 Cells, Labeled Mitoses/100 Mitoses, Labeled Degenerates/100 Degenerates, Labeled Mitoses/100 Labeled Cells, and Labeled Degenerates/100 Labeled Cells. The indexes for the transplants derived from any of the primary tumors are consistent with each other and are distinct from those of transplants derived from other primaries. With these indexes it is possible to compare the relative size of the labeled and unlabeled cell populations and the rates of growth and degeneration in these populations at various periods after labeling. The data indicate that growth and survival are similar in labeled and unlabeled cells and are stable with time. These results have dual significance: (1) Under the circumstances of these experiments, tritium incorporated into the deoxyribonucleic acid of labeled cells has no demonstrable effect on growth or survival, and (2) as a passive cell marker tritiated thymidine is practical and effective for studying many aspects of tumor growth. To account for the ultimate fate of labeled cells, a model is suggested in which the number of labeled chromosomes in the population remains constant after the first division post labeling. The data from one tumor conform closely to the model in that they follow the prediction that the number of labeled cells does not increase beyond the sixth generation after labeling.
916. Systematic Analysis of Retroviral Vector Integrations in Human Marrow-Repopulating Cells by the Aid of a Data Warehouse
Authors: Stephanie Laufs, K. Zsuzsanna Nagy, Guillermo Guenechea, Frank A. Giordano, Africa Gonzalez-Murillo, Kurt Fellenberg, Agnes Hotz-Wagenblatt, Juan A. Bueren, W. Jens Zeller, Stefan Fruehauf
Journal: Molecular Therapy (2004)

Increasing use of hematopoietic stem cells for retroviral vector-mediated gene therapy and recent reports on insertional mutagenesis in mice and humans have created intense interest to characterize vector integrations on the genomic level. We have studied retroviral integration sites in the genome of human marrow-repopulating cells. Therefore, we transduced peripheral blood progenitor cells (PBPC) from 5 healthy donors with retroviral supernatant of the SF-vector (based on the Friend mink cell-forming/murine embryonic stem-cell virus; transduction efficiency: 7%–41%) and transplanted them into immunodeficient NOD/SCID mice. After 8 weeks of engraftment chimeric mouse bone marrow DNA was analyzed with ligation-mediated PCR (LM-PCR) followed by blast analysis.
182. Towards the Correction of the Genetic Defect in Corneal Keratinocytes from Patients with Macular Corneal Dystrophy Type II
Authors: Stefano Ferrari, Giuliana Ferrari, Claudia Rossi, Annarita Miccio, Nicola Volpi, Fulvio Mavilio, Graziella Pellegrini, Michele De Luca
Journal: Molecular Therapy (2004)
462. Toxicity Related to Predosing with Adenoviral Vectors Carrying Lac-Z or Dominant Negative IKK2 Transgenes
Authors: Viraj P. Mane, Racel Cela, Nicola Brunetti, Phil Ng, Brendan Lee
Journal: Molecular Therapy (2004)

Previous reports (Tao, et al., Molecular Therapy, 3, #1, Jan 2001) (Schiedner et al., Molecular Therapy, 7, #1, Jan 2003) have demonstrated that transient saturation possibly of hepatic Kupffer cells with an adenoviral vector  enhances expression of hAAT or hIFN-beta from adenoviral vectors. In these studies, predoses of LacZ or empty vectors were postulated to temporarily saturate Kupffer cells, lowering the dose threshold for hepatic transduction by Adv-hAAT or Adv-hIFN-beta. Our studies address the potential toxicities associated with using an adenoviral vector as a predose before another adenoviral vector.
The third report of the US Preventive Services Task Force
Authors: Mary Ann Gilligan, Rebekah Wang-Cheng
Journal: BMJ (2003)

New guidelines are timely, accessible, and useful in primary care 

This article originally appeared in BMJ USA 

This month the first four installments in the third US Preventive Services Task Force (USPSTF) report are being published. Unlike the first two reports, published in 1989 and 1996 in single volumes, the third report will appear sequentially and in a variety of formats. Each of the topics reviewed by the Task Force will be available as: 1) a detailed, systematic evidence report published on the Agency for Healthcare Research and Quality's (AHRQ's) web site (www.ahrq.gov/clinic/ uspstfix. htm), 2) a shorter synthesis of the evidence published in a variety of general medicine and family practice journals, and 3) a recommendation and rationale statement (R&R) containing the clinical conclusions derived by the Task Force.

The first four topics reviewed by the Task Force are screening for lipid disorders in adults, chlamydial infection, bacterial vaginosis during pregnancy, and skin cancer. The results are being published in a supplement to the American Journal of Preventive Medicine .1 A clinical review of these four areas appears in this issue of BMJ USA (BMJ USA p 187).2

The primary mission of the USPSTF from its inception in 1984 has been to promote effective clinical prevention. Using …
On-Pump vs Off-Pump Coronary Artery Bypass Grafting—Reply
Authors: John D. Puskas, Candace Palmer-Steele
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
TREATMENT OF ACTIVE RHEUMATOID ARTHRITIS WITH SLOW INTRAVENOUS INJECTIONS OF THYMOPENTIN
Authors: M.G. Malaise, P. Franchimont, R. Bach-Andersen, H. Gerber, H. Stocker, C. Hauwaert, B. Danneskiold-Samsoe, D. Gross, H. Gerschpacher, K. Bolla
Journal: The Lancet (1985)
Emerging arboviral encephalitis
Authors: T. Solomon
Journal: BMJ (2000)

The recent outbreaks of West Nile encephalitis in New York and Israel are drawing the western world's attention to the potential threat of arthropod-borne virus (arbovirus) encephalitis.1 But in many parts of Asia, infection with West Nile virus's sister, Japanese encephalitis virus, is a daily reality.

Epidemics of encephalitis were described in Japan from the 1870s onwards, and Japanese encephalitis virus was first isolated from a fatal case in the 1930s.2 West Nile virus was isolated from the blood of a febrile woman in Uganda a few years later in 1937.3 Both viruses are small enveloped RNA viruses, members of the genus Flavivirus (family Flaviviridae), named after the prototype yellow fever virus ( flavus is the Latin for yellow). The flaviviruses are relatively new viruses, derived from a common ancestor 10-20 000 years ago, that are rapidly evolving to fill new ecological niches.4 Both West Nile and Japanese encephalitis virus are transmitted in an enzootic cycle between small birds by Culex mosquitoes, though for Japanese encephalitis …
In-Office Opiate Treatment "Not a Panacea"
Authors: Brian Vastag
Journal: JAMA (2003)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Incidence of In Situ and Invasive Melanoma in Denmark From 1985 Through 2012
Authors: Neel Maria Helvind, Lisbet Rosenkrantz Hölmich, Sigrun Smith, Martin Glud, Klaus Kaae Andersen, Susanne Oksbjerg Dalton, Krzysztof Tadeusz Drzewiecki
Journal: JAMA Dermatology (2015)

In Denmark, the incidence of malignant melanoma (MM) has doubled during the past 25 years, with an incidence of 29.5 and 31.7 per 100,000 person-years in 2012 for men and women, respectively. Understanding the nature of this increase in incidence is important to optimize prevention, early diagnosis, and treatment of in situ and invasive melanoma in Denmark.To describe changes over time in the incidence and clinical and pathologic characteristics of in situ and invasive melanoma in Denmark from 1985 through 2012.We used the official national Danish Melanoma Group database to describe all eligible, prospectively registered cases of in situ and invasive melanoma in Denmark from January 1, 1985, through December 31, 2012. Data analyses were performed from April 1, 2012, through January 31, 2013.Estimated annual percentage changes (EAPCs) for men and women in European age-standardized incidence, age at diagnosis, and tumor region for in situ melanoma and MM. For MM only, melanoma type, Breslow thickness, ulceration, and mortality.We included 3299 cases of in situ melanoma and 20,760 cases of MM. The incidence (95% CI) of MM increased by 4.5% (3.6%-5.3%) for men and 4.3% (3.5%-5.2%) for women, which was especially pronounced in patients older than 60 years (EAPCs, 5.8% [4.7%-6.8%] and 4.8% [3.8%-5.9%], respectively), in thin (Breslow thickness, <0.75 mm) melanoma (EAPCs, 6.6% [5.0%-8.2%] and 6.1% [6.0%-7.1%], respectively), and in superficially spreading MM (EAPCs, 5.2% [4.3%-6.2%] and 4.7% [3.9%-5.7%], respectively). We found no significant EAPC in the incidence of melanomas with Breslow thickness greater than 2.00 mm in women, and relative ulceration rates (95% CI) declined in both sexes (EAPCs, -3.3% [-4.0% to -2.6%] in men and -3.4% [-4.0% to -2.8%] in women). More proximal tumor location occurred over time (P < .001). Incidence of in situ melanoma (95% CI) greatly increased (EAPCs, 14.0% [12.2%-15.8%] in men and 11.6% [10.2%-13.2%] in women) with changes over time in age and region (defined by codes in the International Statistical Classification of Diseases, Tenth Revision) similar to those for MM. Mortality related to MM increased in men (EAPC, 0.6% [0.1% to 1.2%]), whereas mortality in women (EAPC, -0.4% [-1.0% to 0.3%]) remained stable.This study confirms a worldwide increase in melanoma incidence. Results may indicate the importance of secondary melanoma prevention in Denmark. Future efforts could intensify primary prevention aimed at young adults, adolescents, and children and maintain and target secondary prevention at the population older than 60 years.
Abstract P436: Correlates of Physical Activity at 3- and 12-Months Postpartum
Authors: Catherine J Vladutiu, Kelly R Evenson, Anne Marie Jukic, Amy H Herring
Journal: Circulation (2014)

Background: Participation in at least 150 minutes/week of moderate-intensity aerobic activity is suggested for postpartum women. However, few women engage in these recommended levels of physical activity after pregnancy. To inform the development of interventions aimed at maintaining or increasing physical activity in the postpartum period, it is important to understand which factors are associated with postpartum physical activity. Methods: We examined the correlates of self-reported moderate to vigorous physical activity (MVPA) at 3- and 12-months postpartum among a cohort of women in the Pregnancy, Infection, and Nutrition Postpartum study. Women self-reported the frequency, duration, and mode of MVPA. Potential correlates were identified according to the socioecological framework and included sociodemographics, behavioral characteristics, non-recreational modes of MVPA (assessed only for recreational MVPA), reproductive history, infant care, psychosocial factors, and barriers to MVPA. Logistic regression models with GEE were used to identify correlates of any MVPA (≥10 minutes/week across all modes of activity) and any recreational MVPA (≥10 minutes/week) among women at 3-months (n=667) and 12-months (n=530) postpartum. Results: At both time points, lower odds of participation in any MVPA were associated with lower education, breastfeeding, depression, and minimal emotional support. Low confidence in exercise ability, receipt of advice about physical activity, and warmer seasons were associated with higher odds of participation in any MVPA. Associations varied by time point for two correlates. Low poverty and odds of any MVPA were negatively associated at 3-months and positively associated at 12-months postpartum. History of preeclampsia and odds of any MVPA were positively associated at 3-months and negatively associated at 12-months postpartum. For recreational MVPA at both time points, lower odds of participation were associated with unmarried status, lower education, employment, low poverty, history of preeclampsia, and minimal emotional support. Involvement in child/adult care activities and warmer seasons were associated with higher odds of participation in any recreational MVPA. Associations varied by time point for two correlates. Transportation MVPA and odds of any recreational MVPA were positively associated at 3-months and negatively associated at 12-months postpartum. Moderate stress and odds of any recreational MVPA were negatively associated at 3-months and positively associated at 12-months postpartum. Conclusion: These findings suggest that there are several modifiable factors associated with participation in MVPA in the postpartum period. Selected associations varied by time point. This information should be considered when developing interventions to help women maintain or increase physical activity following pregnancy.
Tuberculosis of Bone and Joint.
Authors: G. R. Girdlestone, Edgar W. Somerville
Journal: JAMA (1952)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 16416: Long-term Clopidogrel Therapy Improves Endothelial Function in Stable Coronary Artery Disease
Authors: Scott R Willoughby, Lee-Jen Luu, James D Cameron, Adam J Nelson, Stephen G Worthley, Matthew I Worthley
Journal: Circulation (2010)

Background: Recent data has shown clopidogrel therapy may be beneficial in reducing cardiovascular adverse events in patients with established atherothrombotic disease. Several studies have suggest...