tbuckley/cabot-search huggingface dataset:
Abstract 518: Characterization of Carotid Atherosclerosis: A Comparison Between PET Activity, Plaque Morphology and Histopathology
Authors: Amparo L. Figueroa, Ricardo C. Cury, Udo Hoffmann, Thomas J. Brady, Ahmed Tawakol
Journal: Circulation (2009)

Background: While vascular FDG uptake correlates with inflammation, its relationship to plaque morphology remains unclear. Accordingly, we tested the hypothesis that FDG uptake is higher in segment...
Abstract 539: Molecular and Functional Characterization of Novel Hypertrophic Cardiomyopathy Susceptibility Mutations in TNNC1-Encoded Troponin C
Authors: Andrew P Landstrom, Michelle S Parvatiyar, Jose R Pinto, Michelle L Marquardt, J. M Bos, Steve R Ommen, James D Potter, Michael J Ackerman
Journal: Circulation (2007)
Identification of epigenetically silenced genes in lung adenocarcinoma cell lines
Authors: Gerwin Heller, Barbara Ziegler, Andrea Gsur, Martin Bilban, Christoph Zielinski, Sabine Zochbauer-Muller
Journal: Cancer Research (2007)

2858   Lung cancer is the leading cause of cancer deaths in the world. Aberrant methylation (referred to as methylation) of promoter regions of certain tumor suppressor genes (TSGs) has been identified as an alternative mechanism to mutations and deletions for loss of gene expression in lung cancer. In an approach to better understand the role of aberrant DNA methylation in lung adenocarcinomas we investigated so far the expression patterns of the lung adenocarcinoma cell line A549 before and after treatment with the DNA methyltransferase inhibitor 5-aza-&2acute;-deoxycytidine (Aza-dC) and/or the histone deacetylase inhibitor trichostatin A (TSA) by DNA microarray analysis. The increase of expression was defined as > 4-fold change in expression compared to untreated cells. We found 147 genes out of 22283 probe sets with an increase in expression after Aza-dC treatment, 373 genes with an increase in expression in TSA-treated cells and 513 up-regulated genes after Aza-dC/TSA treatment compared to untreated cells. As expected, we observed that expression of several genes which are involved in different cancer-associated pathways was induced after drug treatment. These genes included RARα, RARRES1 and RARRES3 (involved in retinoic acid signalling), BIK (involved in apoptosis), the putative tumor suppressor genes HYAL-1, BLU, KLF4, CD82 and CGREF1 and the metastasis suppressor MTSS1. However, besides direct induction of gene expression by DNA demethylation and histone acetylation we hypothesize that drug treatment of cells results in up-regulation of certain genes due to secondary effects. Thus, we plan to perform ChIp on Chip analysis using specific antibodies against the methylcytosin binding proteins MeCP2 and MBD2 to identify methylated regions throughout the entire genome of lung adenocarcinoma cell lines. By comparing the results of the gene expression microarrays and the ChIp on Chip analyses we will be able to identify both aberrantly methylated genes and genes whose expression is induced due to secondary effects of drug treatment. Our results will lead to a better understanding of mechanisms which are involved in the pathogenesis of lung adenocarcinomas and the molecular effects of drugs which target certain epigenetic changes.
The Conundrum of Training in Global Surgery
Authors: Mamta Swaroop, Catherine deVries
Journal: JAMA Surgery (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal
The synergistic effect of autologous tumor-stimulated T lymphocyte with trastuzumab for breast cancer.
Authors: Toshiro Yahara
Journal: Cancer Research (2008)

AACR Annual Meeting-- Apr 12-16, 2008; San Diego, CA

2140 

To investigate a synergistic effect on human breast cancer cells and the clinical responses between the autologous tumor-stimulated T lymphocytes (AuTL) in combination with trastuzumab, we applied intra-peritumoral AuTL injection to 14 patients with a refractory recurrent breast cancer simultaneously with trastuzumab. The clinical response showed the PR/NC in 1/7 patients, carcinomatous pleural effusion was well controlled in 6 and 5 patients. The adverse effects of the immunotherapy were tolerable. These results suggested the synergistic enhancement of anti-tumor effect could be induced between AuTLs and trastuzumab, in patients with refractory recurrent breast cancer. Thus this immunotherapy will be applicable.
Abstract 13657: The REG1 Anticoagulation System Allows for Early Arterial Sheath Removal After Cardiac Catheterization Without Increases in Time to Hemostasis or Bleeding Complications: An Analysis From the RADAR Clinical Trial.
Authors: John P Vavalle, Thomas J Povsic, Roxana Mehran, Christopher E Buller, Jan H Cornel, Christoph Bode, Mike Brown, Jaroslaw D Kasprzak, John L Petersen, Gilles Montalescot, Steven L Zelenkofske, John H Alexander, Mauricio G Cohen
Journal: Circulation (2011)
Prognostic Value of ST-Segment Elevation in Lead aVR in Patients With Type A Acute Aortic Dissection
Authors: Masami Kosuge, Keiji Uchida, Kiyotaka Imoto, Susumu Isoda, Norihisa Karube, Toshiaki Ebina, Kiyoshi Hibi, Hidefumi Nakahashi, Kengo Tsukahara, Noriaki Iwahashi, Nobuhiko Maejima, Munetaka Masuda, Satoshi Umemura, Kazuo Kimura
Journal: Journal of the American College of Cardiology (2015)
Abstract 3426: The Generation Of Biosynthetic Small Caliber Grafts With Improved Patency Using Autologous Genetically Modified Endothelial Cells.
Authors: Meir Preis, Jacob Schneiderman, Belly Koren, Tzafra Cohen, Dana Levin Ashkenazi, Rona Shofti, Basil S Lewis, Yosef Shaul, Moshe Y Flugelman
Journal: Circulation (2007)

Introduction: Seeding polymer-based synthetic vascular grafts with endothelial cells (EC) improves grafts biocompatibility and consequently grafts patency. EC adhesion and survival on the synthetic polymer remains a considerable challenge. We used pseudo-typed retroviral vectors to modify EC to co-express fibulin-5 and VEGF 165 and created a unique EC phenotype with increased adhesion and enhanced proliferation and used these cells to seed vascular grafts. Methods: Polymer-based grafts seeded with autologous venous EC transduced to co-express fibulin-5 and VEGF 165 were implanted in the carotid arteries of the donor sheep. 18 grafts (8 seeded grafts with EC co-expressing fibulin-5 and VEGF, 6 seeded with control EC and 4 bare grafts) were implanted for two week in nine sheep. Six months studies were performed on 18 grafts (6 study, 6 seeded with control EC and 6 bare) implanted in 18 sheep. Histological studies for cell adhesion, intra-luminal thrombosis and transgene expression were tested in implanted grafts after angiography. Results: At two weeks all 8 grafts seeded with EC co-expressing fibulin-5 and VEGF were patent with no significant thrombosis and 73% coverage by seeded EC, while 3/10 control grafts were thrombosed and only 30% of seeded unmodified EC remained on grafts luminal surface. At 6 months, 5/6 grafts seeded with EC co-expressing fibulin-5 and VEGF were patent while 9/12 control grafts (6 bare, 6 seeded with unmodified EC, p&lt;0.04) were occluded. At 6 months, the genetically modified cells were identified on the graft surface and these grafts had improved EC coverage and reduced intra-luminal neo-intima and thrombosis. No local or systemic side effects were attributed to the implanted grafts. Conclusion : Polymer-based vascular grafts seeded with EC transduced to co-express fibulin-5 and VEGF have lower propensity to thrombosis and improved patency. Use of grafts seeded with EC co-expressing fibulin-5 and VEGF provides a potentially clinically relevant method for small caliber blood vessel engineering.
Abstract 3136: Characterization of Mortality Risk Associated with Surgery Following Drug Eluting Stent Use
Authors: Saif Anwaruddin, Mike Militello, Ryan Christofferson, John Galla, Matthew Becker, Samir Kapadia, Arman Askari, Stephen Ellis
Journal: Circulation (2007)

Introduction:  There is mounting evidence of stent thrombosis and associated adverse events following cessation of dual antiplatelet therapy in patients with drug eluting stents (DES). However, the risk of adverse events in relation to surgical procedures following DES remains less well-defined.   Methods:  We identified all patients that have had percutaneous coronary intervention (PCI) with DES at Cleveland Clinic since 2003 and had an inpatient surgical procedure at any time point after PCI. Baseline demographic information including antiplatelet therapy status at time of surgery was collected. Social security death index was used to obtain mortality data. The primary endpoint was the risk of all-cause mortality following surgery.   Results:  485 patients underwent PCI with DES followed by index surgical procedure at Cleveland Clinic. The 30-day mortality rates were significantly different depending on time from PCI to surgery. Baseline characteristics based on time to surgery from PCI are shown in the table. A significantly higher proportion of patients going to surgery within one month of PCI were on clopidogrel at least 72 hours prior to surgery.   Conclusion : This is the first description of short-term mortality estimates following surgical procedures after DES. Mortality is significantly increased if surgery has to be performed within 30 days of DES implantation. However, in this cohort there was no difference in mortality after this time period.
Abstract 2335: Transposition Of The Great Arteries And Double Outlet Right Ventricle Have Related Early Developmental Origins: Fundamental Insights From <i>In Vivo</i> Imaging
Authors: Colin K Phoon, Ruiping Ji, Piyali Dhar Chowdhury
Journal: Circulation (2007)

Congenital heart defects (CHD) are the most common birth defect. Proper retinoic acid (RA) signaling is required for normal development, and RA excess produces conotruncal CHD such as transposition of the great arteries (TGA) and double outlet right ventricle (DORV). We hypothesized that origins and evolution of TGA and DORV follow specific developmental patterns, definable in vivo by noninvasive ultrasound biomicroscopy (UBM)-Doppler imaging. Timed-pregnant ICR mice were injected with 70 mg/kg IP RA at 8.5 days post-conception (E8.5). Individual embryos were tracked with 40 MHz UBM-Doppler longitudinally on 5 consecutive days (E11.5–15.5: N=34 injected, N=8 uninjected controls). CHD included TGA (24%) and DORV (21%, all with right aorta, left pulmonary artery), as well as ventricular septal defect (VSD, defined at E15.5) and double-inlet left ventricle. External gross examination and histology confirmed ~90% concordance of UBM diagnosis of CHD, here (at E15.5) and in separate experiments (E11.5–15.5, N=111). All E11.5 embryos showed normal cardiac looping, with a normal unseptated heart and outflow tract (OFT) - the truncus arteriosus -arising from the right ventricle/bulbus cordis. Of embryos destined for TGA, 7/8 showed partial septation of the distal truncus into parallel OFT at E12.5; 4 showed DORV at E12.5 before evolving into TGA by E13.5. Of embryos destined for DORV, 6/7 showed partial septation of the distal truncus into parallel OFT at E12.5 as well. Thus, abnormal septation commences in the more distal truncus arteriosus as early as E12.5. In contrast, embryos destined for normal OFT development showed partial OFT septation in spiral (non-parallel) fashion, with complete/near-complete OFT septation and morphology at E13.5. All TGA/DORV embryos displayed normal cardiac function. CONCLUSIONS. TGA and DORV are developmentally related, differing only in complete (TGA) vs. incomplete (DORV) leftward truncal shift. Given normal heart, proximal OFT, and cardiac function, flow streams do not explain TGA/DORV morphogenesis. Corresponding to 4–6 weeks’ human gestation, these first in vivo data support longstanding hypotheses of TGA/DORV embryogenesis, and implicate RA signaling in the pathogenesis of abnormal truncal septation and shift.
Abstract 1411: Doxorubicin Triggers Replicative Senescence and Death of Cardiac Progenitor Cells
Authors: Elena Piegari, Antonella De Angelis, Toru Hosoda, Donato Cappetta, Laura Marino, Cynthia Carrillo-Infante, Amelia Filippelli, Liberato Berrino, Konrad Urbanek, Jan Kajstura, Annarosa Leri, Francesco Rossi, Piero Anversa
Journal: Circulation (2008)

Doxorubicin (DOXO) is a very effective drug for the treatment of neoplastic diseases. However, DOXO causes a cardiomyopathy that leads to congestive heart failure. To test whether DOXO affects the cardiac progenitor cell (CPC) compartment, CPCs were exposed to increasing doses of the anthracycline for 12, 24 and 48 hours. At 0.1 μ M, DOXO did not alter CPC survival. However, 0.5 and 1 μ M DOXO reduced progressively and dramatically CPC viability. Apoptosis measured by TdT assay, DNA laddering and caspase-3 activity increased markedly with time peaking at 48 hours. The impact of DOXO on CPC growth was determined by BrdU and phospho-H3 labeling. The percentage of BrdU-positive CPCs and the mitotic index decreased with increasing doses of DOXO and time. The expression of the molecular regulators of G1 and G1\S transition, cyclin D1 and cdk4, decreased progressively with increasing concentration of DOXO and time, and these changes were coupled with a severe reduction in phospho-Rb in CPCs. Also, DOXO resulted in a transient increase of the cell cycle inhibitor p21 Cip and a persistent increase in p16 INK4a in CPCs. To determine whether the generation of reactive oxygen species (ROS) was responsible for DNA damage in CPCs, the presence of 8-OH-deoxyguanosine (8-OHdG) was measured. DOXO treatment strikingly increased the fraction of CPCs positive for 8-OHdG. Moreover, the expression of the antioxidant enzymes manganese superoxide dismutase and catalase failed to increase and counteract ROS-mediated DNA damage. Severe telomeric shortening occurred in CPCs exposed to DOXO. Telomere attrition was also favored by a significant decrease in telomerase activity in DOXO-treated CPCs. Dysfunctional telomeres trigger a DNA damage response in which the major determinant is the expression of p53. Both the transcription factor p53 and its target gene p21 Cip1 were upregulated in DOXO-treated CPCs. These molecular adaptations of CPCs increased with the dose and time of exposure to the anthracycline. In conclusion, these observations support the notion that DOXO-induced heart failure is a stem cell cardiomyopathy.
Abstract 2308: Conventional Aortic Valve Replacement in Very Elderly Patients
Authors: Gerald M Lawrie, Elizabeth A Earle, Nan R Earle
Journal: Circulation (2008)

Suboptimal early and late results of percutaneous AVR have been justified by its use in patients claimed to be at high risk for conventional surgery, particularly the very elderly. We performed an analysis to identify which risk factors in conventional AVR patients are most predictive of mortality and to quantify those risk factors in order to provide a realistic baseline for comparison. We analyzed the outcome of surgery in 1168 patients operated on over a 31 year period on one service. Of those patients, 50 suffered perioperative mortality. Of those, 54% (27/50) were male vs. 62% (699/1118) who did not suffer perioperative mortality and 32% (16/50) vs 22% (242/1118) were &gt;76 yrs old; 21% (9/43) vs. 25% (241/982) had diabetes, and 9.3% (9/43) vs. 5.5 (54/982) had bacterial endocarditis (all p=NS); 22% (11/50) vs. 11% (122/1118) had a prior MI (p=0.0152); 26% (13/50) vs 11% (118/1118) had a prior CAB (p=0.0007); 46% (23/49) vs 29% (322/1118) had a concurrent CAB (p=0.0063); mean EF of 44.50±18.26 vs. 53.33±13.90 (p&lt;0.0001); and mean replacement valve size of 20.69±2.14 vs. 21.75±2.46 (p=0.0031). Logistic regression analysis identified prior CAB (odds ratio (OR) 1.65. p&lt;0.01), concurrent CAB (OR 1.63, p&lt;0.01), bacterial endocarditis (OR 1.85, p&lt;0.05) and replacement valve size (OR 0.81, p&lt;0.01 – larger size is protective) as predictors of perioperative mortality. Age was not a predictor of perioperative mortality. Cox regression analysis for factors predictive of overall mortality identified age (relative risk (RR)1.046, 4.6%/yr, p&lt;0.0001) preop EF (RR 0.982, decrease risk of 1.8%/1% increase EF, p&lt;0.0001), diabetes (RR 1.254, p=0.0031, 25% increase risk with diabetes), and replacement valve size (RR 0.889, p=0.0004, 11.1% decreased risk/mm valve size.) These data suggest that even in high-risk patients, perioperative mortality is relatively low and is not predicted by age alone; therefore conventional surgery should be seriously considered in almost all patients. Age is only one of several risk factors which should be evaluated.
Exposure to Fall-out Radiation
Authors: G MANLEY, P W KENT
Journal: BMJ (1964)

Exposure to Fall-out Radiation Some months ago we noted' that the levels of strontium-90 in milk in 1963 might be higher than in 1962 and this has indeed proved to be the case.Methods for analysing strontium-90 in biological materials are normally so time- consuming that actual measurements up to the end of 1963
Abstract 10814: Standardized Preoperative Corticosteroid Treatment in Neonates Undergoing Cardiac Surgery- Results From a Randomized Trial
Authors: Eric M Graham, Andrew M Atz, Ryan J Butts, Nathaniel L Baker, Sinai C Zyblewski, Rachael L Deardorff, Scott T Reeves, Scott M Bradley, Francis G Spinale
Journal: Circulation (2010)

Background: A heightened inflammatory response occurs following cardiac surgery in neonates (≤30 days). The perioperative use of glucocorticoids, such as intravenous methylprednisolone (MP), has be...
Abstract 3190: Gender Differences in Long-term Prognosis of Patients with Known or Suspected CAD and Severe LV Dysfunction Undergoing Gated Myocardial Perfusion SPECT
Authors: Aiden Abidov, Sean W Hayes, John D Friedman, Xingping Kang, Louise E Thomson, Ishac Cohen, Romalisa Miranda-Peats, Piotr Slomka, Guido Germano, Daniel S Berman
Journal: Circulation (2007)

Objective : We aimed to assess the impact of gender on long-term prognosis in pts with known or suspected CAD and impaired vs. preserved left ventricular (LV) function assessed by means of gated myocardial perfusion SPECT (gSPECT). Methods . 575 consecutive pts (38% women, mean age 66±12 yrs;43% pharmacological stress) had rest/stress gSPECT and were then followed up for 11±1 yrs. 55 pts (9.6%) had severe LV dysfunction (post-stress LVEF≥35%). Follow-up end-point was all-cause death (ACD). Prognostic value of clinical and gSPECT variables in predicting ACD was assessed by multivariable Cox proportional hazards analysis. We also evaluated tested variables for presence of confounding and interactions. Results . During the follow-up, there were 143 ACD events (24.9%). Compared to men, women were younger, had less severe perfusion defects, higher LVEF, and smaller LV volumes. There was as a significant interaction between female gender and LV function; survival of women with LVEF≥35% was significantly worse compared to men with the same level of LV impairment, even after adjustment for all other significant predictors, such as age, perfusion defect extent/severity, abnormal ECG, rest HR, dyspnea, history of known CAD and diabetes. (Figure ; *p&lt;0.001). No difference found among the male and female pts with LVEF&gt;35%. Conclusion : In pts with severe LV dysfunction (within the so-called “AICD range”), female gender is associated with worse long term prognosis, independent of other prognostic markers and despite more favorable clinical profile. If confirmed in prospective studies, these findings may have important clinical implications in management of pts with severe LV impairment.
Abstract 1367: The Nad(p)h Oxidase Component Nox4 Is Partially Localized In Mitochondria In Cardiac Myocytes
Authors: Tetsuro Ago, Junya Kuroda, Jayashree Pain, Junichi Sadoshima
Journal: Circulation (2008)

Reactive oxygen species (ROS) regulate cell growth, death, and differentiation in cells. NAD(P)H oxidases are major sources of ROS in the cardiovascular system. Among the NAD(P)H oxidase protein family (Nox1–5), Nox4 is expressed in both the heart and cultured cardiac myocytes. However, the function of Nox4 in myocytes remains unknown. In order to examine expression of Nox4 in myocytes in detail, we made mouse monoclonal Nox4 antibodies, using the C-terminal cytoplasmic region of Nox4 (residues 307–578) as the antigen. After screening more than 1,000 clones, we chose a clone (3D2) that detected recombinant and endogenous Nox4 in mouse hearts and in neonatal rat cardiac myocytes without crossreacting with other Nox isoforms. Endogenous Nox4 was detected as a 55 kD protein in cardiac myocytes in immunoblot analyses. Immunocytochemistry showed that endogenous Nox4 is localized in the peri-nuclear region in myocytes. When HA-tagged Nox4 was expressed in myocytes, staining with HA antibody and that with the Nox4 antibody exhibited an overlapping pattern in the peri-nuclear region. Interestingly, staining of endogenous and exogenous Nox4 was co-localized with that with Mitotracker, a mitochondrial marker. Fluorescent intensity of Mitosox, an indicator of superoxide production in mitochondria, was enhanced in myocytes overexpressing Nox4. Consistently, in mice with cardiac-specific overexpression of Nox4 (Tg-Nox4), expression of Nox4 was increased 2.5 fold in the mitochondrial fraction. Superoxide dismutase-inhibitable NAD(P)H-dependent superoxide production in the mitochondrial fraction was greater in Tg-Nox4 than in non-transgenic mice (NTg) (1.7 fold). Proteomic analysis demonstrated that mitochondrial proteins, related to the TCA cycle and the electron transport, such as mitochondrial aconitase and NADH dehydrogenase, are strongly oxidized in Tg-Nox4, compared to NTg. These results indicate that Nox4 may be partially localized in mitochondria. Although leakage from electron transport chain accounts for the majority of superoxide production in mitochondria, Nox4 may participate in the superoxide production in mitochondria thereby modulating mitochondrial oxidative stress in cardiac myocytes.