tbuckley/cabot-search huggingface dataset:
P2-15-11: Outcomes of Corrective Procedure with Vicryl Mesh as an Oncoplastic Surgery of the Breast.
Authors: YA Lim, YO Lee, JH Kim, HS Kim, HJ Kang, LS Kim, Sacred Hallym
Journal: Cancer Research (2011)

Abstract Background: Both cosmetic and oncologic outcomes are becoming more important for breast cancer patients. Breast-conserving surgery (BCS) has an acceptable cosmetic result compared with mastectomy, but BCS is also associated with cosmetic failure. In this study, we evaluated the cosmetic outcome of reconstructive surgery with the absorbable implant vicryl mesh after BCS compared with the outcome of BCS alone. Methods: From May 2007 to December 2009, 101 patients were involved in this study at Hallym University Sacred Heart Hospital. We used absorbable implants made of folding vicryl mesh and wrapped them with Interceed. In 79 cases, BCS with vicryl mesh implantation was performed; in the other 56 cases, only BCS was performed. Patient satisfaction with cosmetic outcomes was evaluated. We also analyzed other factors that affected cosmetic outcomes, including patient ages, body mass indices, tumor locations, and removed breast tissue. Results: In the vicryl mesh implantation group, 65 of 79 patients (82.3%) were satisfied; in the BCS-only group, 24 of 56 patients (43%) were satisfied (P &amp;lt; 0.05). In the vicryl mesh implantation group, patient ages, body mass indices, and removed breast tissue did not affect cosmetic outcomes. When the tumor was located in the upper outer quadrant, the patients were more satisfied (P &amp;lt; 0.05). Conclusions: Reconstructive procedures with vicryl mesh are simple, safe, and less expensive than other plastic reconstruction techniques. This study suggests that the procedure was superior to BCS alone in cosmetic outcomes. We believe that the procedure could become a favorable technique in oncoplastic surgery. Citation Information: Cancer Res 2011;71(24 Suppl):Abstract nr P2-15-11.
Abstract 15460: Pulmonary Hypertension is Associated with Apoptosis, the Downregulation of S100A1, the Upregulation of S100A6 and the Receptor for Advanced Glycation end-Products
Authors: James Tsoporis, Vassilios Salpeas, Nikos Kavatzas, Shehla Izhar, Jean-Francois Desjardins, Krystyna Teichert-Kuliszewska, Ioannis K Rizos, Thomas G Parker
Journal: Circulation (2014)

Pulmonary arterial hypertension (PH), defined by a resting mean pulmonary artery pressure (PAP) &gt;25 mm Hg, is a syndrome in which pulmonary arterial obstruction increases pulmonary vascular resistance, leading to right ventricular (RV) hypertrophy, failure, and death. Development of PH involves the complex interactions of multiple cell effectors. The possibility that the S100 calcium binding proteins S100A1, S100A6 and their receptor the receptor for advanced glycation end-products (RAGE) may be involved PH and associated apoptosis has not been tested. We examined the levels of S100A1, S100A6, S100B, RAGE and parameters of apoptosis the anti-apoptotic Bcl2 and the pro-apoptotic Bax and terminal deoxynucleotidyl transferase dUTP nick end labelling assay (TUNEL) positive nuclei in lung biopsies negative for malignancy taken by video-assisted thorascopic surgery in 6 control patients (4 male, 2 female) mean age 59.8±4.4 (SEM) years with PA&gt;60 mmHg. S100A1 mRNA and protein as quantified by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and histological evaluation respectively is decreased 50-60%, whereas RAGE and S100A6 mRNA and protein is increased in the whole lung predominantly in PA endothelial and smooth muscle cells approximately 4-10 fold in PH patients compared to controls. Lung S100B levels did not differ between the control and PH groups. The PH group showed a 2.1-3.5 fold increase in the whole lung BAX/BCL2 mRNA ratio and TUNEL positive nuclei compared to controls. Compared to wild type (WT), 10-12 week old S100A1-knockout mice (KO) exhibited an increased right ventricular weight as quantified by the Fulton index (WT-0.24+0.01 vs. KO-0.28+0.01, p&lt;0.05, n=6/group), an elevation in right ventricular systolic pressures (WT-15.5+3.8 vs. KO-28.8+4.6 mmHg, p&lt;0.05) in the absence of altered left ventricular filling pressures, a 1.5-fold increase in lung caspase-3 activity as a marker of apoptosis and 2-3 fold increase in whole lung RAGE and S100A6 mRNA expression. Our data demonstrating that absence of S100A1 results in PH describes a novel murine model of PH, which displays many of the hallmarks of the human disease, thus makes it an attractive therapeutic target in the treatment of PH.
Abstract 17244: Monocytes and Oxidized Lipids Promote the Destabilization of Calcified Plaque Via Matrix Metalloproteinases(MMP)
Authors: Rongsong Li, David Mittelstein, Rohit Majumdar, Juhyun Lee, Yin Tintut, Linda Demer, Tzung Hsiai
Journal: Circulation (2011)

Objective: Vascular calcification is closely associated with atherosclerosis, but whether it mechanically affects plaque stability remains controversial. We previously showed that calcific nodules produced with cultured calcifying vascular cells (CVC) were destabilized in response to shear stress. Here, we characterized the CVC-derived calcified nodules in ApoE-null mice, and assessed oxLDL and monocytes on the destabilization of CVC-derived calcified nodules under pulsatile shear stress (PSS). Methods and Results: To demonstrate that CVC-derived calcified nodules contain components of calcific atherosclerotic plaque in vivo, CVC was implanted in the ApoE-null mice via a cage. After 6 weeks, histology and immunohistochemsitry revealed a calcified plaque-like structure with fibrous caps, lipids, Collagen II, alkaline phosphotase and friable shoulders. To investigate the calcific nodule destabilization in vitro , CVC nodules were subjected to PSS for two and one-half hours. The percentage of nodules detached was calculated as quantification of nodule destabilization. Computational Fluid Dynamic (CFD) code simulated the instantaneous shear force distribution on the individual nodules and demonstrated accentuated instantaneous shear forces on the shoulder regions of nodules. Treatment with oxLDL or co-culture with monocytic THP-1 cells promoted the destabilization of nodules(Nodules detached: control (PSS) = 24±3%, PSS + THP-1=57±6%, PSS + oxLDL=45±7%, n=4, P &lt;0.001). Both oxLDL and THP-1 significantly up-regulated the expression of MMP-1, MMP-3 and MMP-9 in CVC, and increased the activities of MMP. MMP inhibitor, GM6001, significantly reversed oxLDL- and THP-1-induced nodule destabilization, whereas over-expression of MMP9 increased nodule destabilization. Conclusion: These findings revealed that calcific CVC nodules resembled atherosclerotic plaque in vivo , and active lipids and monocytes induced CVC nodules to express MMP, promoting destabilization of the calcified nodules. This study provides a dynamic model to characterize the destabilization of calcific atherosclerotic plaque in vitro .
Human Rh D monoclonal antibodies (BRAD-3 and BRAD-5) cause accelerated clearance of Rh D+ red blood cells and suppression of Rh D immunization in Rh D- volunteers
Authors: BM Kumpel, MJ Goodrick, DH Pamphilon, ID Fraser, GD Poole, C Morse, GR Standen, GE Chapman, DP Thomas, DJ Anstee
Journal: Blood (1995)
A comparison of quality of life in patients with T3 rectal cancer receiving short course versus long course preoperative radiation. A Trans-Tasman Radiation Oncology Group Trial (TROG 01.04)
Authors: S. McLachlan, R. Fisher, B. McClure, J. Beresford, J. McKendrick, J. Mackay, M. Solomon, B. Burmeister, C. Hartopeanu, S. Ngan
Journal: Journal of Clinical Oncology (2008)

4097 Background: A randomised trial was conducted comparing the efficacy of short course (SC) and long course (LC) radiotherapy, prior to surgery in patients with resectable T3 rectal cancer. The impact of these treatments on quality of life (QL) was evaluated. Methods: QL was measured using the EORTC QLQ C-30 and QLQ C-38. Assessments were made in both groups at baseline, 1, 2, 3, 6, 9, 12 months. The C-30 global health/overall QL subscale was specified as the primary outcome. Mean change in QL scores from baseline for each domain, by arm was examined and pointwise differences compared using t-tests and p <0.01 for significance. Results: 326 patients were accrued from 27 Australasian centres. 287 were assessable for QL. Questionnaire completion at baseline, 1, 2, 3, 6, 9, 12 months was; 98%, 80%, 80%, 85%, 83%, 79%, 78% respectively. At baseline there were no between treatment differences for the C-30 or C-38. Global health/overall QL declined in both arms, particularly in the postoperative period (20 points). When compared with respect to the date of surgery, QL profiles were very similar with the exception that there was a trend for SC to be worse 4 months following surgery (difference = 13.2; p=0.036). Similar patterns pertained to fatigue, anorexia, pain and physical, role, social and sexual functioning. At 12 months most QL scores by treatment returned, on average, to within 10 points of their baseline value. Exceptions included sexual enjoyment and male sexual function (much worse) and future perspective (much better). At 12 months there were few between-treatment differences in change from baseline scores (none with p < 0.010). Conclusions: Preoperative radiotherapy and surgery adversely affect several aspects of QL. The most significant decline occurs in the post operative period. By 12 months most aspects of QL have returned close to baseline values with little difference between treatment groups. No significant financial relationships to disclose.
Abstract 705: <i>In vivo</i> audition of tumor homing peptides using high-throughput sequencing and q-PCR
Authors: Kadri Toome, Tarmo Mölder, Kuldar Kõiv, Pille Säälik, Kazuki N. Sugahara, Erkki Ruoslahti, Tambet Teesalu
Journal: Cancer Research (2014)

Abstract In vivo peptide phage display has been widely used to explore tumor vascular heterogeneity and to develop targeting ligands for the delivery of anticancer drugs and imaging agents. Numerous tumor homing peptides with various targeting specificities have been identified. To select a suitable targeting ligand for a particular tumor from an existing peptide panel, it is crucial to perform unbiased quantitative assessment of in vivo peptide homing. Here we describe an approach for parallel evaluation of in vivo tumor homing of peptide-displaying phage clones. Mice bearing orthotopic xenografts of gliomas, breast tumors, and prostate tumors were intravenously injected with equimolar mix of phage clones displaying 12 different tumor homing or control peptides. After 30 min circulation and removal of blood by perfusion, representation of each phage clone in tumors and control organs was determined by high throughput sequencing (HTS) and quantitative PCR (qPCR). The ratiometric analysis demonstrated a clear and reproducible homing signature for each tumor model. Calibrated copy number data obtained by qPCR correlated well with the HTS analysis. Selected phage clones were validated for tumor homing by conventional single-phage homing studies using immunofluorescence, phage titration, and qPCR. Our study shows that the ratiometric in vivo phage biodistribution analysis can be used to rapidly identify peptides that efficiently deliver payloads to particular tumor types. As the peptides are evaluated side-by-side in a same animal, inter-animal variability is eliminated and the number of animals required is dramatically decreased. The choice of analysis (HTS vs qPCR) depends on the complexity of phage pool to be audited. HTS enables the analysis of highly complex phage pools, but introduces a potential bias owing to multiple DNA amplification steps. On the other hand, qPCR allows precise quantification of the copy number of the target sequences, and is suitable for low complexity pools. Narrowing down a complex pool with HTS followed by precise evaluation with qPCR can be a potential combination of the two analytical methods. Citation Format: Kadri Toome, Tarmo Mölder, Kuldar Kõiv, Pille Säälik, Kazuki N. Sugahara, Erkki Ruoslahti, Tambet Teesalu. In vivo audition of tumor homing peptides using high-throughput sequencing and q-PCR. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 705. doi:10.1158/1538-7445.AM2014-705
Abstract 13453: Macrophage-driven Expression of IL-37 Protects Against Atherosclerosis
Authors: Sara McCurdy, Yvonne Baumer, Emma Toulmin, William Boisvert
Journal: Circulation (2014)

As the most prominent cell type in the pathogenesis of atherosclerosis, macrophages are the first cells to infiltrate into the intima where they induce inflammatory events that further exacerbate the disease. We have found recently that the human protein IL-37, a relatively unknown member of the IL-1 family of cytokines with no known mouse homolog, has potent anti-inflammatory activities. Accordingly, we sought to determine if macrophage-specific expression of IL-37 would be effective in curbing atherosclerosis via its ability to reduce the production of pro-inflammatory cytokines, reduce transmigration, and to regulate the accumulation of lipids in mouse bone marrow-derived macrophages (BMDM), ultimately proving to reduce the development of atherosclerosis in a mouse model of the disease. We achieved robust and long-term expression of IL-37 using a macrophage-specific retroviral overexpression system utilizing the CD68 promoter in mouse BMDMs via transduction of hematopoietic stem cells (HSC). Cytokine expression was measured by antibody array as well as RT-qPCR, transmigration of transduced BMDM was measured using a transwell filter assay, and lipid homeostasis by cholesterol uptake as well as efflux. The mouse study was accomplished through bone marrow transplantation (BMT) of transduced wt HSC (IL-37 vs GFP ctl, N=14) into irradiated ldlr-/- mice, with subsequent induction of atherosclerosis development over 10 weeks of HFD-feeding. IL-37 expression reduced the production of pro-inflammatory cytokine transcript and protein, reduced macrophage transmigration, as well improved macrophage cholesterol homeostasis. In vivo, mice that received IL-37-transduced HSC transplants showed significantly less plaque formation than mice that received GFP-transduced control HSCs. The potent anti-inflammatory as well as anti-atherogenic properties of IL-37 make it an attractive target for possible future therapy against atherosclerosis as well as other inflammatory conditions.
IMPAIRED PERIPHERAL ENDOTHELIAL FUNCTION AS ASSESSED BY DIGITAL REACTIVE HYPEREMIA PERIPHERAL ARTERIAL TONOMETRY AND RISK OF IN-STENT RESTENOSIS
Authors: Naohiro Komura, Kenichi Tsujita, Kenshi Yamanaga, Kenji Sakamoto, Sunao Kojima, Shinji Tayama, Koichi Kaikita, Seiji Hokimoto, Kiyoshi Hibi, Kazuo Kimura, Satoshi Umemura, Hisao Ogawa
Journal: Journal of the American College of Cardiology (2014)
The effect of albumin globulin, pus and dead bacteria in aerobic and anaerobic conditions on the antibacterial activity of moxifloxacin, trovafloxacin and ciprofloxacin against Streptococcus pneumoniae, Staphylococcus aureus and Escherichia coli
Authors: E. Rubinstein, L. Diamantstein, G. Yoseph, G. Gruzman, B. Rubinovitch, A. Barzilai, N. Keller
Journal: Clinical Microbiology and Infection (2000)
Thigh-Length Versus Below-Knee Stockings for Deep Venous Thrombosis Prophylaxis After Stroke
Authors: M. Dennis, G. Cranswick, A. Deary, Alec Fraser, Christopher Graham, Stephen Michael Grant, A. Gunkel, J. Hunter, A. MacRae, Damon Perry, V. Soosay, Clare Williams, A. Williamson, Ashley Young, P. Sandercock, J. Reid, G. Murray, G. Venables, Anthony Rudd, Gerald Bowler, M. G. Celani, S. Ricci, R. Lindley, M. Hautvast, Martin Paterson, T. Ting, C. Baigent, J. Bamford, J. Slattery, Clots Trial Collaborat
Journal: Annals of Internal Medicine (2010)

Background: Graduated compression stockings are widely used for deep venous thrombosis (DVT) prophylaxis. Although below-knee stockings are used more often than thigh-length stockings, no reliable evidence indicates that they are as effective as thigh-length stockings. Objective: To compare the effectiveness of thigh-length stockings with that of below-knee stockings for preventing proximal DVT in immobile, hospitalized patients with stroke. Design: Parallel-group trial with centralized randomization (minimization within centers) to ensure allocation concealment. The ultrasonographers who looked for DVT were blinded, but the patients and caregivers were not. (Controlled-trials.com registration number: ISRCTN28163533) Setting: 112 hospitals in 9 countries. Patients: 3114 immobile patients hospitalized with acute stroke between January 2002 and May 2009. Intervention: 1552 patients received thigh-length stockings and 1562 patients received below-knee stockings to wear while they were in the hospital. Measurements: Ultrasonographers performed compression duplex ultrasonography in 1406 patients (96% of survivors) in each treatment group between 7 and 10 days after enrollment. They performed a second scan in 643 patients in the thigh-length stockings group and 639 in the below-knee stockings group at about 25 to 30 days. The primary outcome was symptomatic or asymptomatic DVT in the popliteal or femoral veins, detected on either scan. Results: Patients were retained in their assigned group for all analyses. The primary outcome occurred in 98 patients (6.3%) who received thigh-length stockings and 138 (8.8%) who received below-knee stockings (absolute difference, 2.5 percentage points [95% CI, 0.7 to 4.4 percentage points]; P = 0.008), an odds reduction of 31% (CI, 9% to 47%). Seventy-five percent of patients in both groups wore the stockings for 30 days or until they were discharged, died, or regained mobility. Skin breaks occurred in 61 patients who received thigh-length stockings (3.9%) and 45 (2.9%) who received below-knee stockings. Limitation: Blinding was incomplete, 2 scans were not obtained for all enrolled patients, and the trial was stopped before the target accrual was reached. Conclusion: Proximal DVT occurs more often in patients with stroke who wear below-knee stockings than in those who wear thigh-length stockings. Primary Funding Source: Medical Research Council of the United Kingdom, Chief Scientist Office of the Scottish Government, and Chest Heart and Stroke Scotland.
Cancer- and Treatment-Related Anemia Clinical Practice Guidelines in Oncology
Authors: George M. Rodgers, David Cella, Asher Chanan-Khan, Carolyn Chesney, Charles Cleeland, Peter F. Coccia, George D. Demetri, Benjamin Djulbegovic, Jennifer L. Garst, Margaret Gore, Eric H. Kraut, Weei Chin Lin, Michael Millenson, Victoria Mock, Denise Reinke, Joseph Rosenthal, Paul Sabbatini
Journal: Journal of the National Comprehensive Cancer Network (2005)

Cancer- and treatment-related anemia has received increasing attention, particularly because relationships between anemia and quality of life (QOL) or treatment outcomes are under investigation. This guideline recognizes the multiple etiologies of cancer- and treatment-related anemia, but specifically addresses the treatment of anemia caused by the myelosuppressive effects of chemotherapy and the anemia associated with chronic disease. It provides guidelines for the use of erythropoietic agents in patients with cancer who are receiving chemotherapy. For the most recent version of the guidelines, please visit NCCN.org
A Dilemma in the Glycosaminoglycan‐Based Therapy: Synthetic or Naturally Unique Molecules?
Authors: Vitor H. Pomin
Journal: Medicinal Research Reviews (2015)

Abstract Glycosaminoglycans (GAGs) are widely explored in the biomedical market as functional ingredients in pharmaceutical or nutraceutical preparations. This extensive application of GAGs is justified by their multiple activities across several systems including, but not limited to, coagulation, thrombosis, inflammation, cancer, angiogenesis, cell differentiation, tissue repair, and microbial infections. Therapeutic GAGs are commonly extracted from mammalian tissues. Although functional in diverse systems, mammalian GAGs present serious downsides in therapy such as contamination risk from the mammalian tissues. In order to overcome some of the downsides, two new GAG sources have been appearing as alternatives to the mammalian‐derived molecules. They are the synthetic GAGs and those extracted from nonmammalian origins such as invertebrate animals. This report overviews the general aspects of each GAG alternative and compares critically their pros and cons attributes in light of the prospects for the future of GAG‐based therapy.
AACVPR/ACC/AHA 2007 Performance Measures on Cardiac Rehabilitation for Referral to and Delivery of Cardiac Rehabilitation/Secondary Prevention Services
Authors: Randal J. Thomas, Marjorie King, Karen Lui, Neil Oldridge, Ileana L. Piña, John Spertus, Robert O. Bonow, N.A. Mark Estes, David C. Goff, Kathleen L. Grady, Ann R. Hiniker, Frederick A. Masoudi, Ileana L. Piña, Martha J. Radford, John S. Rumsfeld, Gayle R. Whitman
Journal: Journal of the American College of Cardiology (2007)
Abstract 54: A Comparison of Blood Pressure-Lowering Treatment Decisions Based on Levels of Blood Pressure, Age, and Risk
Authors: Kunal N Karmali, Donald M Lloyd-Jones, Joep van der Leeuw, David Goff, Kazem Rahimi, Johan Sundstrom
Journal: Circulation (2014)

Introduction: The goal of primary prevention is to direct treatment to individuals who will derive greatest benefit. However, single risk factors such as blood pressure or age incompletely capture absolute risk and potential treatment benefit for the individual, thus providing a role for multivariable risk assessment. In this study, we aim to compare blood pressure treatment decisions based on levels of systolic blood pressure (SBP), age, or pretreatment risk. Methods: We used individual participant data from participants without cardiovascular disease in the Blood Pressure Lowering Treatment Trialists’ Collaboration, a prospective overview of randomized blood pressure trials. Three treatment strategies based on thresholds of SBP, age, or five-year Framingham risk estimates were compared by calculating expected number of cardiovascular events and net benefit using graphical methods and decision curve analyses. Strategies were compared as differences in the areas under the curves expressed as percentages of the maximum possible area, with bootstrap-obtained confidence intervals. Results: We analysed 16,419 individuals with 53,571 person-years of follow-up. At every level of number of individuals treated or number of events expected, the risk-based approach using the Framingham risk estimates appeared superior to SBP (risk minus SBP-based treatment decisions = -2.8% [95% CI -4.1% to -1.3%]) or age (risk minus age-based decisions -2.6% [95% CI -4.7% to -0.7%]) (Figure). For a treatment threshold based on expected absolute risk reduction, net benefit was maximized using Framingham risk estimates for a threshold of 1 to 4%, although the differences did not meet statistical significance. Conclusions: Blood pressure treatment decisions based on estimated absolute risk appear more efficient than age- or SBP-based treatment decisions, across a wide range of treatment thresholds. These results support prioritizing risk-based blood pressure treatment strategies in the primary prevention of cardiovascular events.
Elevated Thromboxane Levels in the Rat during Endotoxic Shock
Authors: J. A. Cook, W. C. Wise, P. V. Halushka
Journal: Journal of Clinical Investigation (1980)

The potential deleterious role of the proaggregatory vasoconstrictor, thromboxane A2, in endotoxic shock was investigated in rats. Plasma thromboxane A2 was determined by radioimmunoassay of its stable metabolite thromboxane B2. After intravenous administration of Salmonella enteritidis endotoxin (20 mg/kg), plasma thromboxane B2 levels increased from nondetectable levels (<375 pg/ml) in normal control rats to 2,054±524 pg/ml (n = 8), within 30 min to 2,071±429 at 60 min, and decreased to 1,119±319 pg/ml, at 120 min. Plasma levels of prostaglandin E also increased from 146±33 pg/ml in normal controls (n = 5) to 2,161±606 pg/ml 30 min after endotoxin (n = 5).