tbuckley/cabot-search huggingface dataset:
Abstract 12932: Day of the Week and Acute Heart Failure Admissions: Relation With Acute Myocardial Infarction, Length of Stay and Readmission Rate
Authors: Mahek Shah, Soumya Patnaik, Carlos Davila, Marvin Lu, Parichart Junpaparp, Saranya Buppajarnthum, Toni Anne De Venecia, Aditya Chandorkar
Journal: Circulation (2014)

Background: The day of the week has been inconsistently linked to the length of hospital stay (LOS) and even mortality in patients with heart failure (HF). With Medicare expenditure for HF hospitalizations exceeding 17 billion USD a year, there has been an increased focus on measures that could decrease HF readmissions. While reduced physician staffing, poor quality of care and lesser supervision may be seen on weekends, its role in predicting a worse outcome is unclear. Methods: All patients with HF as the admitting diagnosis between 1/1/2003 and 12/31/2013 were included. The admissions were stratified based on the day of admission and an association with outcomes as LOS, new acute myocardial infarction (AMI) or readmission for HF was analyzed. Results: From the 22,287 admissions included, 4,874 (22%) were on the weekend. The average age (69+/-15 years in both groups, p=0.98) and risk factors as hypertension, diabetes, hyperlipidemia, chronic kidney disease and coronary artery disease were similar between weekend and weekday admissions. More male admissions were seen on weekdays (48% vs. 46%, p=0.034) and the patients admitted on weekends experienced 25% more AMIs (p=0.005). The mean daily admission rate was significantly higher on weekdays (6.1+/-0.8/day vs. 4.2+/-0.6/day, p&lt;0.001) with Monday and Sunday having the highest and lowest rates (6.58/day vs. 4.23/day respectively). There were lesser patient discharges on weekends than weekdays (13% vs. 87%). The LOS was similar for weekday and weekend admissions (5.7+/-6.1 vs. 5.5+/-5.8 days, p=0.125) but Friday was associated with the longest stay in the week (6+/-6.1 days vs. 5.6+/-6 days, p=0.001). The maximum and minimum 30 day readmission rate was seen in patients who were discharged on Friday and Saturday respectively with 18% more readmissions in those discharged on Friday compared to Saturday. Conclusions: Weekends may see lesser HF admissions than weekdays but the risk of an AMI is higher in these patients. Friday admissions were associated with the maximum LOS and Friday discharges with the highest readmission rate. A better understanding of the hurdles faced during weekends with directed research and subsequent remediation of the issues can potentially improve HF care and related clinical outcomes.
Multipronged Approach Helps Alleviate Poverty
Authors: M. J. Friedrich
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Bleeding from the eyes and through intact skin: Physiologic, structural, spiritual, or faked?
Authors: Stephanie K. Bezner, George R. Buchanan
Journal: American Journal of Hematology (2013)

Patients with an apparent bleeding disorder can usually be diagnosed by a careful history, physical examination, and screening laboratory tests. However, at times the constellation of bleeding signs and symptoms fail to be explained by test results and/or our current understanding of hemostatic mechanisms. One such patient is the subject of the current report. She is a 13-year-old female with a history of striking bleeding manifestations, including spontaneous hemorrhage from her eyes, scalp, hands, and feet. She was evaluated by one of the authors at a teaching hospital in Mumbai, India in March 2009 during the filming of a National Geographic Channel documentary characterizing puzzling medical disorders encountered in India. Given her unusual bleeding manifestations, she received international media attention at the time. National Geographic and a film company in the United Kingdom subsequently expressed interest in highlighting the patient to document her seemingly rare hematologic disorder and contacted the American Society of Hematology to identify an American hematologist to further investigate the case. With consent of the family and collaboration with a hematologist practicing at a teaching hospital in Mumbai, filming commenced during March 2009 in an attempt to capture the patient's diagnosis and the cultural and medical milieu in which the bleeding events occurred. Am. J. Hematol. 88:713–716, 2013. © 2013 Wiley Periodicals, Inc.
Abstract 3024: Cancer stem-like cells in ovarian clear cell carcinoma are enriched in ALDH-high population associated with the accelerated scavenging system of reactive oxygen species
Authors: Tomoko Mizuno, Noriko Suzuki, Minako Mori, Hiroshi Makino, Tatsuro Furui, Naoki Ito, Akio Yamamoto, Kennichiro Morishige
Journal: Cancer Research (2014)

Abstract In ovarian cancer cases, disease recurrence after chemotherapy is frequently observed, suggesting ovarian cancer stem-like cells (CSCs) are involved. Ovarian clear cell carcinoma is especially chemo-resistant among epithelial ovarian cancer subtypes. We first, examined the CSC markers (CD133,CD44, ALDH1) using the ovarian clear cell carcinoma tissues (n=81). Immunohistochemical staining revealed that high ALDH1 expression levels are related to advanced stage in clear cell carcinoma cases. Other makers are not related to clinical staging and prognosis. Clear cellcarcinoma cells (KOC-7C) are separated to ALDH-high and ALDH-low population by ALDEFLUOR assay and fluorescence-activated cell sorting. ALDH-high cells showed more quiescent character (G0/G1 phase) than ALDH-low cells. Recent studies have suggested that CSCs might have a high antioxidant capacity to keep cellular reactive oxygen species (ROS) at a low level that allows them to survive adverse conditions. We examined the cellular ROS level using Cell-ROX assay. ALDH-high cells contained the lower levels of ROS than ALDH-low cells. Also, ALDH-high cells showed an increased expression of in nuclear factor-E2-related factor 2 (Nrf2), a key transcriptional factor of the antioxidant system, associated with the increased antioxidant enzymes (SOD2, HO-1). Thus, ALDH-positive CSCs might have increased Nrf2-induced antioxidant scavengers, which lower ROS level relevant to chemo-resistance in ovarian clear cell carcinoma. Citation Format: Tomoko Mizuno, Noriko Suzuki, Minako Mori, Hiroshi Makino, Tatsuro Furui, Naoki Ito, Akio Yamamoto, Kennichiro Morishige. Cancer stem-like cells in ovarian clear cell carcinoma are enriched in ALDH-high population associated with the accelerated scavenging system of reactive oxygen species. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3024. doi:10.1158/1538-7445.AM2014-3024
Abstract 3564: A new method for preparation of low-input, PCR-free next generation sequencing libraries
Authors: Laurie Kurihara, L. Banks, S. Chupreta, C. Couture, V. Kelchner, J. Laliberte, S. Sandhu, R. Spurbeck, V. Makarov
Journal: Cancer Research (2014)

Abstract Sequence analysis of PCR-free Next Generation Sequencing (NGS) libraries demonstrates improved evenness of coverage relative to NGS libraries that have undergone PCR amplification. However, existing PCR-free methods require 1ug or more of input DNA quantity in order to obtain sufficient library yield which restricts such analysis when DNA sample quantity is insufficient. To expand the applicability of PCR-free sequencing, Swift Biosciences has developed a novel adapter attachment chemistry that is more efficient than existing methods. This new technology, Accel-NGS™, results in libraries that capture a higher complexity of each sample which enables a lower input DNA requirement. For Illumina systems, PCR-free Accel-NGS libraries can be prepared from 100ng of input DNA for direct sequencing. PCR-free Accel-NGS libraries demonstrate even coverage across a broad GC content as well as when analyzing whole genome sequence coverage. To enable library preparation from even less DNA, Accel-NGS libraries can also be prepared with a low number of PCR cycles for direct sequencing. The Accel-NGS method with PCR results in more even coverage and less PCR duplicates when compared to existing methods that use PCR. For exome hybridization capture, comparable coverage was achieved for Accel-NGS libraries from 100ng DNA input when compared to an existing method that requires 1ug of DNA input. The Accel-NGS method is fast and automatable; additional advantages when applied to clinical samples are under investigation. PCR-free Accel-NGS libraries can also be prepared for Ion Torrent systems from 5ng of input DNA with comparable sequence quality to existing methods. Citation Format: Laurie Kurihara, L. Banks, S. Chupreta, C. Couture, V. Kelchner, J.Laliberte, S. Sandhu, R. Spurbeck, V. Makarov. A new method for preparation of low-input, PCR-free next generation sequencing libraries. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3564. doi:10.1158/1538-7445.AM2014-3564
Cerebral blood flow during delirium tremens and related clinical states studied with xenon-133 inhalation tomography
Authors: R Hemmingsen, S Vorstrup, L Clemmesen, S Holm, P Tfelt-Hansen, A S Sørensen, C Hansen, W Sommer, T G Bolwig
Journal: American Journal of Psychiatry (1988)

The regional cerebral blood flow of 12 patients with severe alcohol withdrawal reactions (delirium tremens or impending delirium tremens) was measured during the acute state before treatment and after recovery. Greater cerebral blood flow was significantly correlated with visual hallucinations and agitation during the acute withdrawal reaction. The results suggest that delirium tremens and related clinical states represent a type of acute brain syndrome mainly characterized by CNS hyperexcitability.
Starting Out, Carrying On
Authors: Dorothy Wall
Journal: JAMA (2002)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 3481: Genetically-engineered mice as a cell line isolating tool
Authors: Hirohiko Kamiyama, Sherri Rauenzahn, Anirban Maitra, James R. Eshleman
Journal: Cancer Research (2014)

Abstract BACKGROUND Isolation of cell lines from patients’ tumors is difficult, due in large part, to contaminating fibroblasts that often overgrow the malignant cells. The same is true of xenografted tumors. To overcome this problem, we developed biochemically selectable mouse cells by using genetically-engineered mice. METHODS We constructed a series of hypoxanthine phosphoribosyl transferase (hprt) null immunodeficient mice. These include nude hprt-null, SCID hprt-null and NOD-SCID hprt-null mice. Tumors from patients were implanted into these mice for growth. During growth of xenografted human cancers, hprt-null murine stromal cells replace their human counterparts to facilitate the growth of human cancer cells. Explanted tumors were then grown in tissue culture flasks with selection media (hypoxanthine, aminopterin, thymine; HAT media) to eliminate the mouse biochemically-defective fibroblasts and produce pure human cancer cell lines. RESULTS We successfully established several pancreatic cancer cell lines and an ovarian cancer cell line from patients using the hprt null immunodeficient mice. CONCLUSIONS The new cell line isolating system using genetically-engineered mice provides a greater chance to isolate pure cancer cell lines from patients’ tumors. This may allow personalized chemotherapy selection. Citation Format: Hirohiko Kamiyama, Sherri Rauenzahn, Anirban Maitra, James R. Eshleman. Genetically-engineered mice as a cell line isolating tool. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3481. doi:10.1158/1538-7445.AM2014-3481
Treatment of viral diseases of the cornea and external eye1This work was supported in part by US Public Health Services grants EY02672 and EY02377 from the National Eye Institute, National Institutes of Health, Bethesda, Maryland, and an unrestricted departmental grant from Research to Prevent Blindness, Inc., New York, New York.The author states that he has no proprietary interest in the development and marketing of any products mentioned in the article or competing ones.1
Authors: Herbert E Kaufman
Journal: Progress in Retinal and Eye Research (2000)
Abstract 11228: The Endothelial Nitric Oxide Synthase Gene -786T/C Polymorphism is a Significant Predictive Factor for Myocardial Lactate Production During Acetylcholine-Provoked Coronary Spasm
Authors: Koichi Kaikita, Koji Sato, Masafumi Nakayama, Kenichi Tsujita, Sunao Kojima, Seiji Hokimoto, Hisao Ogawa
Journal: Circulation (2014)

Myocardial lactate production (MLP) in the coronary circulation is a supporting diagnostic marker useful in the evaluation of coronary spasm-induced myocardial ischemia during acetylcholine (ACh)-provocation test. We previously found a -786T/C polymorphism in the 5’-flanking region of the endothelial nitric oxide synthase (eNOS) gene and reported that this polymorphism is strongly associated with coronary spasm. However, there is no report showing the relation between MLP induced by ACh-provocation test and eNOS gene -786T/C polymorphism in the large study population. We examined 712 consecutive patients who underwent myocardial lactate measurement during ACh-provocation test and agreed with genetic screening test of eNOS gene -786T/C polymorphism between January 1991 and December 2010. ACh-provoked MLP was observed in 252 out of 712 patients included in the present analysis [252 MLP-positive and 460 MLP-negative patients]. MLP-positive patients were more likely to be female, diabetic, non-smokers, had epicardial, multivessel and diffuse spasm pattern, with eNOS-786T/C polymorphism (60% vs. 44%, p<0.001, 25% vs. 19%, p=0.047, 61% vs. 46%, p<0.001, 83% vs. 36%, p<0.001, 37% vs. 11%, p<0.001, 39% vs. 12%, p<0.001, and 23% vs. 15%, p=0.011, respectively). Multiple logistic regression analysis identified female gender, epicardial spasm and -786T/C polymorphism to correlate with the MLP-positive finding (OR: 0.327; 95% CI: 0.226-0.475; p<0.001, OR: 10.137; 95% CI: 6.767-15.187; p<0.001, and OR: 1.621; 95% CI: 1.025-2.564; p= 0.039). In the analysis of patients with epicardial coronary spasm, Kaplan-Meier survival curve showed no difference in 5-year survival rates free from major adverse cardiac events between MLP-positive and MLP-negative patients with epicardial coronary spasm (p=NS). In conclusion, multiple predictors including female gender and eNOS gene -786T/C polymorphism were the significant predictors for the MLP induced by ACh-provocation test, highlighting the close relation between the genetic factors and ACh-induced myocardial ischemia in Japanese patients with coronary vasospastic angina.
Basophil allergen threshold sensitivity, CD‐sens, IgE‐sensitization and DBPCFC in peanut‐sensitized children
Authors: S. Glaumann, A. Nopp, S. G. O. Johansson, M. Rudengren, M. P. Borres, C. Nilsson
Journal: Allergy (2011)

To cite this article: Glaumann S, Nopp A, Johansson SGO, Rudengren M, Borres MP, Nilsson C. Basophil allergen threshold sensitivity, CD‐sens, IgE‐sensitization and DBPCFC in peanut‐sensitized children. Allergy 2012; 67 : 242–247. Abstract Background: Immunoglobulin E (IgE)‐sensitization to peanut is common and can indicate an allergy. A positive test needs to be confirmed by a double‐blind, placebo‐controlled food challenge (DBPCFC), which is regarded as ‘the gold standard’. The aim of the study was to evaluate the basophil allergen threshold sensitivity (CD‐sens) and antibodies to peanut allergen components in relation to DBPCFC in the diagnoses of peanut allergy in children. Methods: Thirty‐eight children with suspected peanut allergy underwent a DBPCFC. CD‐sens to peanut and Ara h 2 were analysed as well as IgE‐antibody to peanut and some of its allergen components (Ara h 1, 2, 3, 8 and 9). Results: Twenty‐five children had a positive DBPCFC, and 92% of these were positive in CD‐sens to peanut and Ara h 2. Two children with a positive DBPCFC were classified as ‘low‐responders’ and were not further evaluated. Children positive in DBPCFC had higher CD‐sens values to peanut (median 1.3; range 0.4–29, n = 21) compared with children negative in DBPCFC (median 0; range 0–0.5, n = 13) ( P &lt; 0.0001). A positive DBPCFC correspond with increased levels of IgE‐antibody to Ara h 1, 2 and 3 compared with those with a negative challenge ( P &lt; 0.0001 for all). All children with a negative CD‐sens were negative in DBPCFC. Conclusion: In this study, a negative CD‐sens to peanut excluded peanut allergy. Both tests, CD‐sens to peanut and immunoassay for IgE‐antibody to the peanut components, appear to be safe, time saving and cost‐effective complements to DBPCFC.
Causes of Traumatic Death During Pregnancy—Reply
Authors: Isabelle L. Horon
Journal: JAMA (2001)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Lab-on-a-chip companion diagnostics for personalized management of breast cancer
Authors: Archana raamanathan, John McDevitt
Journal: Clinical Cancer Research (2008)

B25   Breast cancer is the second leading cause of death among women in the United States. In 2008, an estimated 182,460 new cases will be diagnosed and 40,480 women will die of breast cancer.  HER 2 is a receptor tyrosine kinase oncogene product over-expressed in 30% of breast cancers. HER 2 targeted therapy (Trastuzumab®) has been FDA approved for breast cancer. The extracellular form (ECD) of HER 2 is shed into serum and HER 2 testing has been approved by the FDA to identify eligible metastatic breast cancer patients for Trastuzumab® treatment and follow-up and monitoring of patients with metastatic breast cancer. The promise of personalized medicine in breast cancer is not only to be able to recruit patients for target specific therapy but also to monitor subsequent treatment effectiveness.  In this work, we demonstrate the capability of our Lab-on-a-chip (LOC) multi-analyte biosensor platform in tailoring to the needs of the personalized medicine cancer diagnostics arena. For this proof-of-concept study, we have developed a first generation companion diagnostics chip for HER 2. We also demonstrate multiplexing ability of our platform with a proof-of-concept multiplex assay of HER 2 with CA 125 (prognostic marker for ovarian cancer).  The LOC sensor array platform performs chemical and immunological reactions on and within the interior regions of micro-spheres positioned in the inverted pyramidal micro-chamber wells of a silicon microchip. Micro-fluidic structures deliver a series of small-volume reagents and washes to the chip and to each of the micro-spheres. Optical signals generated by the reactions on the microspheres are visualized by, a charge-coupled device (CCD) video chip along with the use of trans-wafer optics. Using the LOC system, we have developed ultra sensitive sandwich ELISA type immunological assays for these tumor biomarkers. These immunoassays can be performed with small sample volumes and short assay times, and markedly reduced reagent costs thereby enabling rapid, cost effective analysis, imperative for realizing companion diagnostics for delivering personalized therapeutic decisions.  The assays developed have excellent analytical characteristics comparable to commercially available methods and multiplexing abilities rendering them amenable to the development of companion diagnostics in cancer therapy. Future work will entail clinical validation of the assay developed. The lab-on-a-chip platform lends itself to other analyte classes and hence promises a multiplex approach that cuts across several classes of analytes. Access to personalized medicine on such rapid and low cost LOC platforms can truly deliver the promise of fitting ‘the right drug to the right patient’ instead of a ‘one size fits all’ approach. This work will set a precedent for developing future companion diagnostics to serve along with targeted cancer therapeutics.