tbuckley/cabot-search huggingface dataset:
Abstract 4174: Identification of PRPF4 as a novel cancer promoter through AKT signaling in lung cancer
Authors: So-Young Choi
Journal: Cancer Research (2012)

Abstract We recently constructed a large-scale gene expression database containing more than 33,000 tumors samples by applying cancer outlier profile analysis, and identified many candidate genes as therapeutic targets (Shin G et al., 2011). One of potential target candidates is PRPF4, which is over-expressed in a variety of human cancer, particularly in the breast, colon, ovary, skin and lung cancer patients. PRPF4 (PRP4 pre-mRNA processing factor 4 homolog), also called PRP4, HPRP4, Prp4p, or HPRP4P, is involved in pre-mRNA splicing and interacts with U4/U6-U5 tri-snRNP complex. Moreover, there have been a few studies on the roles of PRPF4 in diseases including cancer. In one study, PRPF4, along with MDR1 (ABCB1) and survivins, was involved in drug resistance in ovarian cancer patients (Duan Z et al., 2008), but its role in cancer is mostly unknown. In this study, we found that PRPF4 was over-expressed in breast, colon, and lung cancers, and involved in cancer progression, invasion, and migration, and interacted with AKT. Knock-down of PRPF4 by small interfering RNA in both lung and breast cancer cell lines dramatically reduced invasion, migration, and cell proliferation in vitro. We confirmed the up-regulation of PRPF4 in about 30% of lung and breast cancer specimens by RT-PCR. We also found that PRPF4 interacts with AKT and thus activates PI3K/AKT signaling pathway and increases proliferation, invasion, and migration of lung cancer cells. Collectively, we suggest PRPF4 is a novel up-regulated gene involved in various cancer progression and propose it as a novel cancer biomarker for targeted therapeutics. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4174. doi:1538-7445.AM2012-4174
Cholecystokinin-Cholescintigraphy in Adults: Consensus Recommendations of an Interdisciplinary Panel
Authors: John K. DiBaise, Bryan K. Richmond, Harvey H. Ziessman, Gregory T. Everson, Robert D. Fanelli, Alan Maurer, Ann Ouyang, Peter Shamamian, Richard J. Simons, Laura A. Wall, Thomas J. Weida, Mark Tulchinsky
Journal: Clinical Gastroenterology and Hepatology (2011)
Perioperative Management of Diabetic Subjects: Subcutaneous Versus Intravenous Insulin Administration During Glucose-Potassium Infusion
Authors: Antonio Pezzarossa, Francesco Taddei, M Cristina Cimicchi, Elisabetta Rossini, Sandro Contini, Enzo Bonora, Angelo Gnudi, Enzo Uggeri
Journal: Diabetes Care (1988)

The purpose of this study was to validate methods for the perioperative management of diabetic patients that meet the prerequisites of simplicity, applicability in the absence of a diabetologist, and flexibility, to rapidly meet changing metabolic requirements. The patients were divided into two groups that were comparable for age, sex distribution, type of diabetes, and type of surgical procedures. The results show that intravenous insulin administration achieved better glycemic control during the intraoperative period, whereas it did not offer advantages over the subcutaneous route during the pre- and postoperative periods. The satisfactory degree of steady glycemic control achieved and the absence of hypoglycemic episodes indicate that the separate administration of insulin and glucose plus electrolytes is an effective and safe management modality for diabetic patients undergoing major surgery.
Acute myeloid leukemia with myelodysplasia‐related changes are characterized by a specific molecular pattern with high frequency of <i>ASXL1</i> mutations
Authors: Raynier Devillier, Véronique Gelsi‐Boyer, Mandy Brecqueville, Nadine Carbuccia, Anne Murati, Norbert Vey, Daniel Birnbaum, Marie‐Joëlle Mozziconacci
Journal: American Journal of Hematology (2012)

To determine whether the distinct and heterogeneous WHO category called "AML with myelodysplasia-related changes" (MRC-AML), presents specific molecular alterations we searched for mutations in genes known to be mutated in malignant myeloid diseases. In 48 MRC-AML patients analyzed, we found 17 mutations in ASXL1 (35%), eight in RUNX1 (17%), seven in TET2 (15%), 12 in IDH (n = 2) or IDH2 (n = 10) (25%), four in DNMT3A (8%), four in NPM1 (8%), and one in FLT3 (2%). Mutations were more frequent in the intermediate cytogenetic (IC) subgroup of 36 patients than in the unfavorable karyotype subgroup, with an average ratio mutations/patients of 1.36 [0-3] vs. 0.33 [0-2] (P < 0.001). Then, we compared these 36 patients with IC MRC-AML with a control panel of 37 no-MRC-AML patients, who had both IC and no dysplasia. IC MRC-AMLs were associated with higher incidence of ASXL1 mutations (47% vs. 0%, P < 0.001) and lower incidence of DNMT3A (6% vs. 38%, P = 0.001), NPM1 (11% vs. 62%, P < 0.001) and FLT3 (3% vs. 49%, P < 0.001) mutations. No difference was found in the incidence of IDH1/2 or TET2 mutations according to the presence of dysplasia. Complete remission rate after intensive treatment was lower in the MRC-AML group than in the no-MRC-AML group (48% vs. 78%, P = 0.023) and in wild type NPM1 patients (50% vs. 84%, P = 0.009). Our study showed that MRC-AML as defined in the WHO 2008 classification presents a specific mutation pattern characterized by a high frequency of ASXL1 mutations and a low rate of NPM1, FLT3, and DNMT3A mutations.
Isolation of intestinal mononuclear cells: factors released which affect lymphocyte viability and function.
Authors: P R Gibson, A Hermanowicz, H J Verhaar, D J Ferguson, A L Bernal, D P Jewell
Journal: Gut (1985)

Whether toxic or immunomodulatory factors are released during the collagenase digestion phase of the isolation of mononuclear cells from human intestinal mucosa was investigated by assessing the effect of the collagenase supernatant on the viability and natural killer activity of normal peripheral blood mononuclear cells. Three hours9 incubation in collagenase supernatant suppressed natural killer activity by 25 +/- 4% and decreased the viability of peripheral blood mononuclear cells by 11 +/- 2%. The ability of collagenase supernatants to kill 51Cr-labelled peripheral blood mononuclear cells over four hours was assessed in 16 collagenase supernatants, eight of which produced lysis of 20 +/- 4%. There was no ultrastructural evidence of early degenerative changes in the viable intestinal mononuclear cells fresh from the isolation process or in peripheral blood mononuclear cells incubated in collagenase supernatant. Because prostaglandins are known to inhibit natural killer activity, PGE was measured in 20 collagenase supernatants by radioimmunoassay and found to be high at 27.5 +/- 4.0 ng/ml. Addition of indomethacin to the collagenase medium, however, failed to abolish the inhibitory effect of collagenase supernatant on natural killer activity and did not increase the natural killer activity of isolated intestinal mononuclear cells. The release of cytotoxic and immunomodulatory factors during the isolation of intestinal mononuclear cells indicates the necessity for careful assessment of the potential effects of the isolation process on any function being examined and casts doubt upon the relationship between in vitro findings and in vivo functional capabilities.
Dr. Uplavici and Mr. Flexner
Authors: Sherman M. Mellinkoff
Journal: New England Journal of Medicine (1970)

In 1887 Jaroslav Hlava, distinguished professor of pathological anatomy at Prague, reported his discovery that diarrhea caused by Entamoeba histolytica could be transmitted from man to cats. Professor Hlava's paper, written in the Czech language, was entitled "O úplavici. Předběžné Sdělení," meaning, "On Dysentery: Preliminary communication." In the same year a Dr. S. Kartulis, of Alexandria, reviewed Professor Hlava's report in German and somehow attributed the work to "Uplavici, O." and even referred to correspondence with "Uplavici" in Prague. For the ensuing 50 years investigators and reviewers around the world referred to the classic experiments on amebic dysentery by "Dr. . . .
Chronic Lymphocytic Leukemia With Eyelid Involvement Responding to Alemtuzumab
Authors: Luca Laurenti, Michela Tarnani, Idanna Innocenti, Gustavo Savino, Remo Battendieri, Luigi Maria Larocca, Patrizia Chiusolo, Federica Sorà, Simona Sica, Dimitar Efremov, Giuseppe Leone
Journal: Journal of Clinical Oncology (2008)
Endothelial shear stress identified in vivo within the stent is related to in-stent restenosis and remodeling of stented coronary arteries
Authors: Scott Kinlay, Ahmet U. Coskun, Charles L. Feldman, Maureen E. Clark, Milan Sonka, Andreas Wahle, Olusegun J. Ilegbusi, Jeffrey J. Popma, Richard E. Kuntz, Peter H. Stone
Journal: Journal of the American College of Cardiology (2002)
CLASSES IN NURSING FOR PATRIOTIC WOMEN
Authors: J. M. Shaw
Journal: JAMA (1918)

<h3>To the Editor:</h3> —The magnitude of the conflict that has been raging for more than three years and the part we are to play in it are gradually coming home to our people. A united effort in giving our boys in the trenches and their families at home all available help is becoming increasingly important. As our Army begins active operations, the crying need will be for more trained nurses. Every shipload of soldiers going abroad will emphasize this demand, which will become more and more pressing. As a medical man, I have a few suggestions to make for preparing to meet this situation. Briefly they are as follows: Young women who feel the call of patriotism in this hour of the nation's peril, and who are willing to respond, can be organized into classes of from fifty to one hundred, each class to be given three months' intensive training
Abstract 3180: A fast solution to NGS library preparation with low nanogram DNA input
Authors: Pingfang Liu, Greg Lohman, Eric Cantor, Brad Langhorst, Lynne Apone, Daniela Munafo, Christine Sumner, Fiona Stewart, Thomas C. Evans, Eileen T. Dimalanta, Theodore Davis
Journal: Cancer Research (2012)

Abstract Next generation sequencing (NGS) has significantly impacted cancer genetics, enabling a comprehensive characterization of genomic abnormalities in the cancer genome at very high resolution. By delivering massive DNA sequences at unprecedented speed and cost, NGS promises to make personalized genome-based diagnosis and targeted cancer therapy a reality in the foreseeable future. To date, library construction with clinical samples has been a challenge, primarily due to the limited quantities of sample DNA available. To overcome this challenge, we have developed a fast library preparation method using novel NEBNext reagents and adaptors. This method enables library construction from a minimal quantity of DNA (&amp;lt; 5 ng), and can be used for both intact and fragmented DNA. Moreover, the workflow is compatible with multiple NGS platforms. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3180. doi:1538-7445.AM2012-3180
Expression of CD62L, CD27 and CD11b define a marked cytotoxic effector role for NK cells in rheumatoid arthritis
Authors: M. Abreu, H. Carvalheiro, C. Duarte, J. A. Pereira-da-Silva, M. Souto-Carneiro, P. Rodrigues-Santos
Journal: Annals of the Rheumatic Diseases (2011)

Natural killer (NK) cells play a crucial role in the innate immune response. In the absence of prior activation they are capable of rapidly produce interferon γ (IFNγ) and other immunoregulatory cytokines, as well as lyse specific target cells. Previous studies suggested a dual role for NK cells in rheumatoid arthritis (RA) and limited their characterisation to the surface expression of CD56/CD16. However, the newest information on the heterogeneity and complexity of this cell population has deemed such classification insufficient. In an effort to shed a new light into the possible roles of NK cells in RA in the present study the authors have dissected the heterogeneity of NK by looking into the expression of CD27, CD11b and CD62L as well as the production of pro-inflammatory cytokines in the peripheral blood of RA patients. Peripheral blood NK cells from RA patients (n=10) and healthy donors (n=10) were analysed by flow cytometry, for cell surface expression of CD56, CD16, CD27, CD11b and CD62L, intracellular cytokine production of IFNγ and tumour necrosis factor α (TNFα) and degranulation (CD107a). This data indicate that a higher percentage of CD56<sup>bright</sup> CD27 CD11b and CD56<sup>dim</sup> CD27 CD11b NK cells are present in RA when compared to healthy donors indicating lower maturation status of NK cell compartment, probably due to chronic enhanced stimulation and proliferation. Regarding NK cell CD62L expression, general reduction was found in RA patients, particularly in CD56<sup>bright</sup> NK subset. Better ability for production of IFNγ in CD56<sup>bright</sup> CD16 and CD56<sup>dim</sup> CD16 NK cells was observed in RA patients (28.4%±7.1% vs 11.3%±7.9% and 52.6%±11.5% vs 28.0%±17.3%, respectively). Similar results were obtained for TNFα (26.2%±6.1% vs 14.0%±6.7% and 55.6%±8.9% vs 29.5%±14.5%, respectively). Increased degranulation, based on upregulation of CD107a expression, observed in RA patients CD56<sup>dim</sup> CD16 effector NK cells (41.8% vs 25.9%) could be connected to chronic stimulation in disease. Taken together, this results suggest an important role of CD56<sup>bright/dim</sup> CD27 CD11b NK-cell subsets in RA. These NK cells are potent producers of pro-inflammatory cytokines and thus capable of massive destruction in a chronic inflammation scenario. In line with previous studies, lower expression of CD62L in different RA NK subsets correlates with increased cytotoxicity making NK cell privileged targets for immune therapy.
MEASURING SUDDEN CARDIAC ARREST AND DEATH INCIDENCE IN MINNESOTA HIGH SCHOOL ATHLETES: A COMPARISON OF METHODOLOGY AND IMPLICATIONS FOR PREVENTION STRATEGIES
Authors: K Harmon, J Drezner
Journal: British Journal of Sports Medicine (2014)

Background The incidence of SCD in Minnesota high school athletes was recently reported from 1 in 416,666 to 917,069 athlete-years based on search of catastrophic insurance claims data. The low rate of SCD was attributed to the effectiveness of a standardized pre-participation screening program using American Heart Association recommendations. However, in college age populations insurance claims data identified only 20% of SCDs. Objective To determine the incidence of SCA/SCD in Minnesota high school athletes using media reports and compare this to incidence using insurance claims data. Design Retrospective review of the Parent Heart Watch database generated from systematic search of media reports. Each case was reviewed for details. Sport participation numbers were from the National Federation of High Schools. Setting Minnesota. Participants 1 906 014 high school athletes ages 14–18 from 2002–2012. Interventions None. Main outcome measurements Incidence of SCA. Results Media reports identified 14 SCAs, (7 SCDs /7 SCAs who survived), during the 10 year period examined, all in male athletes. The death rate in Minnesota high school athletes was 1 in 102 485. The SCA rate (death+arrests) was 1 in 51 243 athlete-years and 1 in 33 480 athlete-years in males. 43% of events and 29% of deaths identified occurred while participating on a school sponsored team. Seven of 14 events (50%) occurred in male basketball players for an SCA rate of 1 in 17 465 athlete-years. During the same time period there was one death (14%) identified using catastrophic insurance claims data. Conclusions SCA/SCD rates in Minnesota high school athletes are significantly higher than previously reported and the incidence in male basketball players is disproportionately high. Media reports have been shown to underestimate SCD rates by almost 50% so these numbers represent a low estimate. Insurance claims grossly underestimate the incidence of SCD and cannot be used to measure cardiovascular endpoints in athletes or evaluate screening programs.
von Willebrand Factor and Endothelial Abnormalities in Diabetic Microangiopathy
Authors: Massimo Porta, Macro La Selva, Pia A Molinatti
Journal: Diabetes Care (1991)

We briefly summarize current knowledge on 1) the abnormalities of von Willebrand factor (vWF) as an indicator of endothelial cell (EC) dysfunction in diabetes and 2) the modifications induced in the growth of cultured ECs by high glucose in the incubation media. A MEDLINE search (1986 through Sept. 1989) was performed to update previous relevant references on vWF and ECs in healthy and diabetic subjects. Main data in the literature and personal contributions were scrutinized. Study quality, information, and relevance to the subject were assessed. vWF is synthesized and stored mainly in ECs. Its plasma levels are increased in diabetic microangiopathy but are not influenced by circulating glucose, insulin, or growth hormone, nor do they acutely affect platelet function in diabetes. Supraphysiological concentrations of glucose inhibit the replication of cultured ECs from large vessels via different possible mechanisms but appear to stimulate pathways involved in the activation of capillary ECs. vWF is a possible marker of EC damage in diabetes, and prospective studies will ascertain its role as a predictor for the development of microangiopathy. The possible dichotomy in the response of cultured ECs from large and small vessels to high glucose in the culture media may help explain some of the lesions observed in the walls of arteries and capillaries in diabetes.
Assessment of Short- and Long-Term Mortality Displacement in Heat-Related Deaths in Brisbane, Australia, 1996–2004
Authors: Zhen Qiao, Yuming Guo, Weiwei Yu, Shilu Tong
Journal: Environmental Health Perspectives (2015)

BackgroundMortality displacement (or “harvesting”) has been identified as a key issue in the assessment of the temperature–mortality relationship. However, only a few studies have addressed the “harvesting” issue and findings have not been consistent.ObjectivesWe examined the potential impact of both short- and long-term harvesting effects on heat-related deaths in Brisbane, Australia.MethodsWe collected data on daily counts of deaths (nonaccidental, cardiovascular, and respiratory), weather, and air pollution in Brisbane from 1 January 1996 to 30 November 2004. We estimated heat-related deaths, identified potential short-term mortality displacement, and assessed how and to what extent the impact of summer temperature on mortality was modified by mortality in the previous winter using a Poisson time-series regression combined with distributed lag nonlinear model (DLNM).ResultsThere were significant associations between temperature and each mortality outcome in summer. We found evidence of short-term mortality displacement for respiratory mortality, and evidence of longer-term mortality displacement for nonaccidental and cardiovascular mortality when the preceding winter’s mortality was low. The estimated heat effect on mortality was generally stronger when the preceding winter mortality level was low. For example, we estimated a 22% increase in nonaccidental mortality (95% CI: 14, 30) with a 1°C increase in mean temperature above a 28°C threshold in summers that followed a winter with low mortality, compared with 12% (95% CI: 7, 17) following a winter with high mortality. The short- and long-term mortality displacement appeared to jointly influence the assessment of heat-related deaths.ConclusionsWe found evidence of both short- and long-term harvesting effects on heat-related mortality in Brisbane, Australia. Our finding may clarify temperature-related health risks and inform effective public health interventions to manage the health impacts of climate change.CitationQiao Z, Guo Y, Yu W, Tong S. 2015. Assessment of short- and long-term mortality displacement in heat-related deaths in Brisbane, Australia, 1996–2004. Environ Health Perspect 123:766–772; http://dx.doi.org/10.1289/ehp.1307606
A randomized trial of endoscopic treatment of acute gastric variceal hemorrhage: N-Butyl-2-Cyanoacrylate injection versus band ligation
Authors: Pen-Chung Tan, Ming-Chih Hou, Han-Chieh Lin, Tsu-Te Liu, Fa-Yauh Lee, Full-Young Chang, Shou-Dong Lee
Journal: Hepatology (2006)

Progression of gastric variceal hemorrhage (GVH) is poorer than esophageal variceal bleeding. However, data on its optimal treatment are limited. We designed a prospective study to compare the efficacy of endoscopic band ligation (GVL) and endoscopic N-butyl-2-cyanoacrylate injection (GVO). Liver patients with cirrhosis with or without concomitant hepatocellular carcinoma (HCC) and patients presenting with acute GVH were randomized into two treatment groups. Forty-eight patients received GVL, and another 49 patients received GVO. Both treatments were equally successful in controlling active bleeding (14/15 vs. 14/15, P = 1.000). More of the patients who underwent GVL had GV rebleeding (GVL vs. GVO, 21/48 vs. 11/49; P = .044). The 2-year and 3-year cumulative rate of GV rebleeding were 63.1% and 72.3% for GVL, and 26.8% for both periods with GVO; P = .0143, log-rank test. The rebleeding risk of GVL was sustained throughout the entire follow-up period. Multivariate Cox regression indicated that concomitance with HCC (relative hazard: 2.453, 95% CI: 1.036-5.806, P = .041) and the treatment method (GVL vs. GVO, relative hazard: 2.660, 95% CI: 1.167-6.061, P = .020) were independent factors predictive of GV rebleeding. There was no difference in survival between the two groups. Severe complications attributable to these two treatments were rare. In conclusion, the efficacy of GVL to control active GVH appears not different to GVO, but GVO is associated with a lower GV rebleeding rate. (HEPATOLOGY 2006;43:690–697.)
OB-GYN Advisor
Authors: T. Keith Edwards
Journal: JAMA (1993)

TheOB-GYN Advisoris designed to run on the IBM PC family of computers. The computer system must have at least one MB of space available on a hard disk, 256K RAM, and DOS 2.0 or higher. Installing theOB-GYN Advisoron your system is simply a matter of following the directions in the manual. The software creates its own directory called CRS for Clinical Reference Systems and places it in the root directory of the hard disk.  OB/GYN Advisorstores patient education data on more than 250 obstetric, gynecologic, and new baby topics. These topics may be printed out from the doctor's office computer on demand. The list of topics includes information on surgical procedures, common female and neonatal infections, babysitter guidelines, day-care information, and some general medical problems. A sheet of paper lists these topics in alphabetical order. The list is also contained internally on the hard disk.
dilemma for patient and clinicians
Authors: Samia A Hurst, Alex Mauron
Journal: BMJ (2004)

The story of Maria Tomasa is deeply moving.1 Tommy went through a difficult process to find support in her wish to have a child. Then she risked her health in this pregnancy. Finally, she accepted that her story be told and commented on by strangers. All three steps show admirable courage. The authors must also be commended for publishing a case highlighting several issues on which reasonable people may disagree. Without attempting definitive answers, we comment on a few points illustrated by this story.

One salient element …
Joseph Bartholomew Deasy
Authors: P. Deasy
Journal: BMJ (2012)

Joseph Bartholomew Deasy (“Joe”) was born in Cork City, spent his early life in Cork, and moved to the Dublin area in the early 1930s. He studied medicine at University College, Dublin, and on qualifying he moved to Northumberland to take up the post of resident medical officer at Hexham Emergency Hospital. Here he met Elizabeth (Betty), who was a ward sister at the hospital and …