tbuckley/cabot-search huggingface dataset:
A gene expression signature associated with structural differentiation of prostate acini predicts postoperative recurrence of prostate cancer
Authors: Jimmy J.-M. Su, Chi-Rong Li, Michael T.-L. Lee, Patrick Y. W. Chu, Valerie M. Weaver, Kelvin K.-C. Tsai
Journal: Clinical Cancer Research (2010)

Abstract Glandular malignancies such as prostate carcinoma display a considerable heterogeneity in the degree of structural organization while the prognostic role of the associated molecular changes awaits further exploration. Using a three-dimensional (3-D) tissue organization culture model that permits the structural differentiation of prostate glandular epithelium we profiled the transcriptomes of multicellular organoids formed by untransformed prostate epithelial cells (PECs) or prostate carcinoma cells as well as those cultured as cell monolayers. Interrogation into the multiple (n = 6) sets of differentially expressed genes (DEGs) identified that those altered significantly during the structural differentiation process of polarized PEC acini strongly associated with the probability of recurrence in a cohort of 50 prostate cancer patients receiving radical prostatectomy (RP). By contract, the DEGs related to prostate tumor spheroids did not correlate significantly with prognosis. To further explore this association, we prioritized the genes and applied supervised classification algorithms whereby identified a 39-gene structure-associated prognosis signature (SAPS) that shows a remarkable predictivity for the likelihood of post-RP recurrence (overall accuracy 100%, P &amp;lt; 0.001). The SAPS outperformed standard clinicopathological variables, including Gleason scores, as well as existing multigene prognostic predictors of prostate cancer. Our results suggest a distinct contribution by the glandular structural differentiation program to prostate cancer pathology and the usefulness of a robust and biologically relevant genomic predictor of the prognosis of prostate cancer (Supported by NHRI CA-099-PP-19 and Department of Health of Taiwan H99-TD-C-111-004 to KKC).
SPONTANEOUS HEMOPNEUMOTHORAX; REPORT OF A CASE
Authors: Daniel Snively, Harry Shuman, William D. Snively
Journal: Annals of Internal Medicine (1942)

Case Reports1 February 1942SPONTANEOUS HEMOPNEUMOTHORAX; REPORT OF A CASEDANIEL SNIVELY, M.D., HARRY SHUMAN, M.D., F.A.C.P., WILLIAM D. SNIVELY, M.D.DANIEL SNIVELY, M.D.Search for more papers by this author, HARRY SHUMAN, M.D., F.A.C.P.Search for more papers by this author, WILLIAM D. SNIVELY, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-16-2-349 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptSpontaneous hemopneumothorax, judging by the number of case reports, is a rare condition. Hopkins, 1 reviewing the literature in 1937, found 46 cases reported since 1900. This entity apparently represents a spontaneous pneumothorax in which some unusual condition is present to give rise to bleeding into the pleural cavity. It must be distinguished from traumatic hemopneumothorax and from the effusion of bloodstained serum occasionally seen in malignant growths of the lung and pleura.Our patient presented a classical example of spontaneous hemopneumothorax. Although a total of over 1400 cubic centimeters of blood was removed from his pleural cavity, he made...References1. HOPKINS HU: Spontaneous hemopneumothorax, Am. Jr. Med. Sci., 1937, cxciii, 763. CrossrefGoogle Scholar2. HURSTEPSTEIN AHH: Massive intrapleural hemorrhage as a complication of artificial pneumothorax, Quart. Bull. Sea View Hospital, 1937, ii, 155. Google Scholar3. FREY JL: A case of spontaneous hemopneumothorax, Jr. Am. Med. Assoc., 1935, civ, 1305. Google Scholar4. JONESGILBERT ORCL: Spontaneous haemopneumothorax, Am. Rev. Tuberc., 1936, xxxiii, 165. Google Scholar5. BIRCH CA: A fatal case of spontaneous haemopneumothorax, Brit. Jr. Tuberc., 1936, xxx, 99. CrossrefGoogle Scholar6. HOUSDENPIGGOT EGA: Spontaneous hemopneumothorax with unusual post-mortem findings, Brit. Med. Jr., 1931, ii, 941. CrossrefGoogle Scholar7. HEAD JR: Hemorrhage into the pleural cavity, Surg., Gynec. and Obst., 1937, lxv, 485. Google Scholar8. MILHORAT AT: A case of spontaneous hemopneumothorax (with some simulation of an acute surgical abdominal condition), Am. Jr. Surg., 1931, xiii, 315. CrossrefGoogle Scholar9. LOURIA MR: Spontaneous hemopneumothorax with report of five cases and one autopsy, Quart. Bull. Sea View Hosp., 1938, iv, 44. Google Scholar10. KOROL E: Haemorrhagic pleurisy of tuberculous origin and haemopneumothorax, Am. Rev. Tuberc., 1936, xxxiii, 185. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Rock Island, Illinois*Received for publication October 8, 1940. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byIdiopathic Spontaneous HemopneumothoraxSpontaneous hemopneumothorax with recovery 1 February 1942Volume 16, Issue 2Page: 349-356KeywordsBloodHemorrhageLungs ePublished: 1 December 2008 Issue Published: 1 February 1942 PDF downloadLoading ...
Abstract 17729: Ideal Cardiovascular Health is Associated With a Lower Prevalence of Adverse Left Ventricular Remodeling Patterns in the Framingham Offspring Study
Authors: Matthew Nayor, Danielle Enserro, Ramachandran S Vasan, Vanessa Xanthakis
Journal: Circulation (2014)

Introduction: The American Heart Association Heart Healthy score (AHA score or Life’s Simple 7™) is inversely associated with atherosclerotic vascular disease including heart failure (HF), but its relationship to adverse left ventricle (LV) geometric patterns (known precursors of HF) is unknown. LV geometry can be categorized into 4 distinct patterns: normal (normal LV mass [LVM] and relative wall thickness [RWT]), concentric remodeling (normal LVM with increased RWT), eccentric hypertrophy (increased LVM with normal RWT) and concentric hypertrophy (increased LVM and RWT). Hypothesis: We hypothesized that ideal cardiovascular health is inversely associated with adverse LV geometric patterns. Methods: We evaluated the cross-sectional relations between the AHA score (range 1-7, based on lifestyle and risk factor profile, 7 being healthiest) and the prevalence of echocardiographic LV geometric patterns in 2392 Framingham Offspring Study participants (mean age 58 years, 56% women). Using generalized logits models, we calculated odds ratios (OR) for each pattern of LV remodeling as compared to the normal pattern (that served as referent) for every unit-increase in AHA score, adjusting for age and sex. Results: Normal geometry was present in 1283 participants (54%), concentric remodeling in 486 (20%), eccentric hypertrophy in 352 (15%) and concentric hypertrophy in 271 (11%). Each unit- increase in the AHA score was associated with lower odds of concentric remodeling and hypertrophy, Table. We did not observe a significant association between the AHA score and the odds of eccentric hypertrophy. Conclusions: Our findings support the association between ideal cardiovascular health and a lower prevalence of adverse cardiac remodeling, consistent with the favorable impact of both a healthy lifestyle and optimal risk factor profile on HF risk.
Prevention of hyaline membrane disease.
Authors: M E Avery
Journal: BMJ (1973)

The articles by Liggins and Howie and Baden, et al. in this issue of Pediatrics are of great interest to perinatologists because they describe efforts to extend to premature infants results of recent studies on maturation of lungs of lambs and rabbits in the prevention of hyaline membrane disease. The two reports provide both promise and caution. When labor can be delayed at least 24 hours in infants under 32 weeks, infusion of betamethasone into the mother can prevent respiratory distress in the infant. Results in infants over 32 weeks9 gestation suggest a positive benefit, but the number of infants in the series was too small to reach statistical significance.
Abstract 79: An androgen response element-based reporter assay for the detection of androgen receptor activity in prostate cells
Authors: Waqas Azeem, Margrete R. Hellem, Jan R. Olsen, Yaping Hua, Kristo Marvyin, Lisha Li, Yi Qu, Biaoyang Lin, Xisong Ke, Anne M. Oyan, Karl-Henning Kalland
Journal: Cancer Research (2015)

Abstract Background: The androgen receptor (AR) transcription factor plays pivotal roles in the development and progression of prostate cancer. AR is activated and maintained throughout prostate cancer progression even in castration-resistant prostate cancer (CRPC). AR and its classical target genes such as PSA (KLK3) are, however, efficiently shut off in basal epithelial prostate cells and possibly in prostate cancer stem cells (CSCs). There is limited information on the mechanisms that keep the AR OFF in these cells, and the conditions that turn the AR and target genes ON. Results: Fluorescent and luminescent reporters were constructed to develop a functional assay for AR activity. These reporters contain the repeated AR binding promoter sequences and were designed in a way that the AR can activate these AR element (ARE) reporters. The 241B reporter was selected among the constructed ARE reporters on the basis of higher activity. The AR positive prostate cancer cell line, LNCaP, was transduced with the lentiviral 241B-mCherry fluorescence reporter. FACS analysis quantitated the ARE reporter activity when reporter cells were treated with androgen and with the AR antagonist, enzalutamide, or the anti-androgen, abiraterone. AR expression vectors were also constructed with the AR open reading frames cloned into lentiviral expression vectors. All constructs were sequenced to verify the correct AR DNA sequence and their activity were tested using ARE reporter system. Conclusion: The developed ARE reporter system is useful to understand the mechanisms of AR and AR target gene activation in different subpopulations of prostate cancer cells. It will help us to investigate the role of the AR in differentiation and proliferation of prostate cells and to study the AR activity in two and three dimensional cell cultures. This system can also be useful in screening for drugs with activity against the AR. Citation Format: Waqas Azeem, Margrete R. Hellem, Jan R. Olsen, Yaping Hua, Kristo Marvyin, Lisha Li, Yi Qu, Biaoyang Lin, Xisong Ke, Anne M. Oyan, Karl-Henning Kalland. An androgen response element-based reporter assay for the detection of androgen receptor activity in prostate cells. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 79. doi:10.1158/1538-7445.AM2015-79
New Alzheimer Disease Target Identified
Authors: Joan Stephenson
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
MMR Vaccination and Febrile Seizures—Reply
Authors: Mogens Vestergaard, Kreesten Meldgaard Madsen, Jørn Olsen, Anders Hviid, Jan Wohlfahrt, Mads Melbye, Poul Thorsen, Diana Schendel
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 2933: Assessment of ABT-263 activity across a comprehensive cancer cell line collection leads to a novel, potent combination therapy for small cell lung cancer
Authors: Anthony C. Faber, Carlotta Costa, Anahita Dastur, Cyril Benes, Jeffrey Engelman
Journal: Cancer Research (2014)

Abstract BH3 mimetics like ABT-263 induce apoptosis in subsets of cancer. However, they have shown limited clinical efficacy as single agents, and rational combination strategies remain underexplored. Toward the development of a novel combined therapy, we examined efficacy of ABT-263 across 331 cancer cell lines. Cell lines with high expression levels of the pro-apoptotic gene BIM were more sensitive to ABT-263 activity. This was particularly evident in small cell lung cancer (SCLC), which tends to have higher BIM levels and is among the most sensitive to ABT-263. However, MCL-1 expression mitigated the impact of ABT-263-induced apoptosis by sequestering BIM released from BCL-2 and BCL-XL. Since MCL-1 expression is dependent on TORC1 activity in some cancers, we attempted to overcome the apoptotic block in SCLC by combining ABT-263 with the TORC1/2 inhibitor AZD8055. AZD8055 treatment alone downregulated MCL-1 expression; combination AZD8055 and ABT-263 induced marked apoptosis and growth arrest in vitro, as well as tumor regressions in multiple SCLC xenograft models. Strikingly, low-dose AZD8055 was sufficient to promote tumor regressions in the combination strategy. Therefore, addition of a TORC1/2 inhibitor offers a therapeutic strategy to markedly improve single-agent ABT-263 activity in SCLC. Citation Format: Anthony C. Faber, Carlotta Costa, Anahita Dastur, Cyril Benes, Jeffrey Engelman. Assessment of ABT-263 activity across a comprehensive cancer cell line collection leads to a novel, potent combination therapy for small cell lung cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2933. doi:10.1158/1538-7445.AM2014-2933
Epithelial and Stromal Roles in Carcinogenesis. II. Tumor Development in Internal Transplants of Skin and Epithelium Treated With 3-Methylcholanthrene<xref ref-type="fn" rid="FN1">2</xref>
Authors: Charles A. Ashley
Journal: JNCI Journal of the National Cancer Institute (1961)

The site of action of chemical carcinogens was studied by painting the skin with 3-methylcholanthrene, followed by separate transplantation of the epithelium and stroma from the painted areas. Full-thickness skin was similarly transplanted after carcinogen application. All transplants were placed internally, which thus avoided the uncertainty of the origin of the epithelium occasioned by the ingrowth of neighboring epithelium which occurs when skin is transplanted to external sites. Normal skin and fully developed tumors survived and proliferated after transplantation. Tumors did not develop in the transplants of pure epithelium. Few tumors developed in transplants of full-thickness skin, unless tumors were already present in the skin before it was transplanted. Tumors did develop in the painted sites from which transplants were taken. Experiments have eliminated the strain of mice used, the transplantation site, and transplant necrosis as primary factors in the failure of transplants to develop tumors. Failure was not due to inhibiting factors in the host caused by exposure to carcinogen or by the growth of tumors in the areas painted, since no tumors developed in transplants made to normal mice of the same strain. It was concluded that one of the ultimate steps in carcinogenesis was inhibited by transplantation.
Abstract 260: Nanoparticle-mediated Simultaneous Targeting to Mitochondria and Inflammatory Monocytes Confers Additive Cardioprotection Against Myocardial Ischemia-reperfusion Injury
Authors: Gentaro Ikeda, Tetsuya Matoba, Kaku Nakano, Kenji Sunagawa, Kensuke Egashira
Journal: Circulation Research (2015)

Background: Targeting one mediator of myocardial ischemia-reperfusion (IR) injury failed to reduce infarct size in clinical trials, suggesting the necessity of the innovative strategy to target more than 2 mediators simultaneously. Previously, we have engineered poly(lactic acid/glycolic acid) nanoparticle containing cyclosporine A (CsA-NP) and pitavastatin (Pitava-NP), and reported that the former inhibits the opening of mitochondrial permeability transition pore (mPTP) and the latter reduces monocyte-mediated inflammation in IR hearts. Here we tested the hypothesis that nanoparticle-mediated simultaneous targeting to mPTP and monocytes confers additive cardioprotection against IR injury. Methods and Results: We produced mice deficient with both cyclophilin D (CypD, a key molecule for mPTP opening) and CCR2 (a receptor for monocyte chemoattractant protein-1), and found that the double-KO mice displayed dramatic reduction in myocardial IR injury model (Fig. A). Flow cytometric analysis and fluorescence molecular tomography showed that inflammation was markedly inhibited in CCR2-KO and CypD/CCR2-KO mice while residual inflammation was noted in CypD-KO mice. In CypD mice, Pitava-NP reduced recruitment of Ly6Chigh inflammatory monocytes and showed therapeutic effects (Fig. B). In contrast, CsA-NP reduced IR injury in CCR2-KO mice. Simultaneous treatment with CsA-NP and Pitava-NP at the time of reperfusion showed additive reduction in IR injury in wild-type mice (Fig. C). Conclusions: Nanoparticle-mediated simultaneous targeting to mitochondria and inflammatory monocytes can be developed as a novel therapeutic strategy in IR injury.
Abstract 1649: Minor ST Segment and T Wave Abnormalities Are Associated With Common Carotid Intima-Media Thickness but Not With Coronary Artery Calcium in a Middle-Aged, Biracial Population: The Coronary Artery Risk Development in Young Adults (CARDIA) Study
Authors: Joseph A Walsh, Ronald Prineas, Martha L Daviglus, Kiang Liu, Hongyan Ning, Elsayed Z Soliman, Donald M Lloyd-Jones
Journal: Circulation (2009)
164. Secretion of Human Recombinant Factor IX from Cells of Adipose, Myogenic and Hepatic Origin Transduced with Replication-Defective HSV-1 Genomic Vectors
Authors: Julie Fradette, Ryan Flanigan, David M. Krisky, William F. Goins, Joseph C. Glorioso
Journal: Molecular Therapy (2004)

This project explores an alternative strategy to the current gene therapy approaches for the treatment of hemophilia B. It proposes i) a different vector system based on replication defective herpes simplex virus-1 (HSV), ii) a novel delivery route by transducing adipose cells, transforming them into reservoirs for human Factor IX (hFIX) secretion, iii) a comparison of hFIX secretion after in vitro gene transfer to cells of adipose, myogenic or hepatic origin. Two recombinant HSV vectors expressing the hFIX cDNA under the control of the HCMV promoter were engineered: DHFIX and QHFIX. The QHFIX vector backbone possesses more extensive immediate early gene deletions than DHFIX to minimize any potential vector-related cytotoxicity. We compared the ability of these two vectors to secrete hFIX after transduction of Vero, C2C12, human and rat preadipocytes, human adipocytes, rat primary hepatocytes and HepG2 cells. On average, the transduction efficiency of the QHFIX vector was 26% higher than that observed for the DHFIX vector. Similarly, hFIX secretion levels measured at two days post infection were 1.5 to 11 times higher with QHFIX as determined by ELISA assay on the culture media. QHFIX-transduced preadipocytes produced levels of hFIX similar to that seen for C2C12 cells and rat primary hepatocytes. However, secretion of hFIX from HepG2 cells transduced with QHFIX was 11 times higher than for these other cell types. Mature adipocytes differentiated in vitro and transduced with the QHFIX vector demonstrated high levels of hFIX production for at least 11 days in culture, with a peak of 485 ng/mg/million cells two days after infection. Finally, an ex vivo model of rabbit fat explants maintained in culture was used to evaluate transgene expression and further confirmed the capacity of mature adipose tissue to secrete vector-mediated hFIX after transduction. In conclusion, we consider that transduction of adipose cells by replication-defective HSV vectors producing hFIX could represent a promising new delivery system for expression of coagulation gene products.
Toxic Shock Syndrome
Authors: P. H. Wolf, J. Perlman, J. Fortney, D. Lezotte, R. Burkman, G. Bernstein
Journal: JAMA (1987)

<h3>To the Editor.—</h3> In relating influenza to toxic shock syndrome (TSS), Sperber and Francis<sup>1</sup>further diminish the prospect that barrier contraception will be a major determinant of TSS in this country. Even as multiple causes of TSS are identified, the number of recorded TSS hospital admissions is currently estimated to be less than 1500 nationally.<sup>2</sup> Already, prior surveillance efforts have shown that 60% to 80% of all cases of TSS are associated with menstruation and the use of tampons. The remaining cases of nonmenstrual toxicshock syndrome (NMTSS) occur post partum, in women with surgical wounds, or in women who use barrier contraceptives.<sup>3-6</sup>It now appears that a portion of these cases may be due to postinfluenzal staphylococcal super-infection. A recent National Institutes of Health Center for Population Research workshop on TSS and contraception reviewed the National Institutes of Health—supported national case-finding effort by the Centers for
An outbreak of invasive fusariosis in a children’s cancer hospital
Authors: Nadia Litvinov, Mariama Tomaz N. da Silva, Inneke M. van der Heijden, Mariana G. Graça, Larissa Marques de Oliveira, Liang Fu, Mauro Giudice, Maria Zilda de Aquino, Vicente Odone-Filho, Heloisa Helena Marques, Silvia F. Costa, Anna S. Levin
Journal: Clinical Microbiology and Infection (2014)