tbuckley/cabot-search huggingface dataset:
190. Myocardial Gene Therapy by Naked Angiopoietin-1 Plasmid Injection Efficiently Induces Angiogenesis and Prevents the Remodeling Following Acute Myocardial Infarction
Authors: Takeshi Uzuka, Yoshinori Ito, Masayuki Morikawa, Jinghua Huang, Masayoshi Kobune, Kiminori Nakamura, Yukiko Honma, Keiji Ishii, Tomio Abe, Hirofumi Hamada
Journal: Molecular Therapy (2004)

Background: Angiopoietin-1 (Ang1) is a critical angiogenic factor for vascular maturation and enhances vascular endothelial growth factor (VEGF)-induced angiogenesis in a complementary manner. Previously, we reported that adenoviral vector mediated Ang1 gene therapy clearly promoted myocardial angiogenesis and reduced infarct size in a rat acute myocardial infarction (AMI) model. However, the alternative gene delivery method must explore for clinical application of myocardial Ang1 gene therapy, because of toxicity and inflammatory nature of adenoviral vector. Naked plasmid injection is the safest and convenient method for cardiovascular gene therapy and we have reported that naked CA (CMV enhancer/Chicken β-actin promoter)-promoter can bring remarkable myocardial transgene expression following naked CA-plasmid injection. Therefore, we attempted naked Ang1 plasmid gene therapy for rat AMI model and evaluated myocardial angiogenesis and effects for the remodeling following AMI.
Transplantation of enriched and purged peripheral blood progenitor cells from a single apheresis product in patients with non-Hodgkin's lymphoma
Authors: RS Negrin, CR Kusnierz-Glaz, BJ Still, JR Schriber, NJ Chao, GD Long, C Hoyle, WW Hu, SJ Horning, BW Brown
Journal: Blood (1995)
Fish tank granuloma - A case report from Sri Lanka
Authors: Dushantha Madegedara, Damith Nandadeva, Rajitha Abeysekera, Sumeesha Samarabandu, Rasheeka Liyanaarachchi
Journal: European Respiratory Journal (2014)

We describe a case of  Mycobacterium marinum  infection which started following a cut injury sustained while cleaning a fish tank. A 51-year old patient presented with a history of episodically discharging skin lesion over the right thumb for 10 years duration which had recently started enlarging in size for 3 months duration. He was being treated for focal segmental glomerular sclerosisand his treatment had been changed to ciclosporin 4 months before due to inadequate response.The dormant skin lesion increased in size following this.  A large nodule was observed on the right thumb which had an unhealthy skin over it with healed openings which had drained before  Systemic examination was normal. ESR 52 mm,Mantoux 13mm and CXR was normal. X-ray of the thumb revealed visible soft tissue swelling with no evidence of underlying osteomyelitis or calcification. Skin biopsy histology of the lesion revealed acanthotic epidermis with underlying granulation tissue with ill-defined aggregate of histiocytes and neutrophils. PAS staining for fungi was negative. The appearances were consistent with a fish tank granuloma.  Patient was started on Rifampicin,Ethambutol and Clarythromycinand regression of the lesion was present at 3 months.  Atypical mycobacterial infections are not uncommon. To avoid diagnostic delays, clinicians need to have a high index of suspicion for  M.marinum  infection, especially when patients are immunocompromised.
Hepsin overexpression promotes tumor growth in an orthotopic prostate cancer model
Authors: Wei Li, Bu-Er Wang, Wei-Qiang Gao, Alvin Gogineni, Mary Cole, Leo DeGuzman, Stuart Bunting, Daniel Kirchhofer
Journal: Cancer Research (2007)

AACR Annual Meeting-- Apr 14-18, 2007; Los Angeles, CA

3096 

Hepsin, a type II transmembrane serine protease, is highly upregulated in prostate cancer. Hepsin promotes tumor metastasis in a mouse model of prostate cancer, suggesting a role in the later tumor stages. However, hepsin protein levels are already high in pre-cancerous lesions and remain elevated in the carcinoma and metastasis stages. In this study, we investigated the role of hepsin in primary tumor growth in an orthotopic prostate cancer model by use of hepsin-transfected LnCaP cell lines, which also stably expressed luciferase for bioluminescence imaging. The LnCaP-34 cells stably expressed hepsin protein on the cell surface at about 5-fold higher levels than the LnCaP-17 cells, which only expressed endogenous hepsin. The higher hepsin levels on LnCaP-34 correlated with increased hepsin enzymatic activity towards pro-urokinase type plasminogen activator. 1x106 LnCaP-17 or LnCaP-34 cells were injected into the left anterior lobe of the prostate of SCID-beige mice (20 animals per group) and tumors were analyzed after 16 weeks. We found a higher tumor occurrence (80% vs 58%) and lower body weights (20.8±0.8g vs. 23.5±0.9g; p<0.05) in the LnCaP-34 group compared to the LnCaP-17 group. In addition, animals of the LnCaP-34 group had 2.4-fold higher normalized prostate tissue weights and larger tumors than the LnCaP-17 group (tumor wet weights of 2.12±0.22g and 1.20±0.27g, respectively; p<0.02). Most strikingly, histological examination revealed that the LnCaP-17 tumors were confined to the left anterior lobe of the prostate (7/7 animals), i.e. the lobe where tumor cells were injected at the start of the study, whereas the LnCaP-34 tumors had spread from the left to the right anterior lobes (7/7 animals). These results suggested that hepsin overexpression endowed LnCaP-34 cells with increased invasive capacity concomitant with enhanced tumor growth. Moreover, the in-vitro proliferation rates of LnCaP-17 and LnCaP-34 cells were identical, indicating that the prostate microenvironment was important in mediating the hepsin-driven tumor growth. Unlike the differences in primary tumor growth, the occurrence of lung metastatic lesions as determined by ex-vivo bioluminescence imaging was similar in the LnCaP-17 and LnCaP-34 groups (50% vs 44%). This is in agreement with the presence of numerous tumor cell infiltrates in the tumor vasculature in both groups. However, lymph node metastasis was increased in the LnCaP-34 animals (22% vs 0% in LnCaP-17 animals), based on periaortic lymph node bioluminescence imaging at the time of tissue harvest. In conclusion, the finding that hepsin overexpression enhances invasive tumor growth suggests that elevated hepsin expression in pre-cancerous lesions and in prostate carcinoma may contribute to tumor invasiveness and growth.
Je m'honte
Authors: M. Therese Southgate
Journal: JAMA (2002)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Cilt Ve Cilt Altının Kalsinozisi: Kalsiyum Içeren Düşük Moleküler Ağırlıklı Heparinlerin Subkütan Enjeksiyonuna Karşı Beklenmedik, NonimmünolojikTers Bir Reaksiyon
Authors: Dorothee Eich, Karin Scharffetter-Kochanek, Julis Weihrauch, Thomas Krieg, veNicolas Hunzelmann, Cologne ve Ulm, Almanya
Journal: Journal of the American Academy of Dermatology (2004)
Abstract P5-21-09: Oncologist treatment choices in patients with early stage invasive lobular breast carcinoma - a survey
Authors: Carmel Jacobs, Mark Clemons, Mohamed FK Ibrahim, Christina Addison, Jean-Michel Caudrelier, Ian D Graham, Brain Hutton, Angel Arnaout
Journal: Cancer Research (2015)

Abstract Introduction: Invasive lobular carcinoma (ILC) is common and accounts for 5-15% of all breast cancers. ILC has distinct clinical and histological features that separate it from invasive ductal carcinoma (IDC) with regards to its breast imaging characteristics, patterns of recurrence and sensitivity to systemic therapy. ILC presents challenges to the physician in many aspects of local-regional and systemic therapy choices. We surveyed breast cancer physicians on their beliefs and practice patterns on issues around the management of ILC. Methods: A questionnaire was developed and circulated electronically using a modified Dillman technique to surgical, radiation, and medical oncologists across Canada and Ireland. Results: The questionnaire was completed by 91 of 429 physicians (21% response rate). Response rate by specialty was 25/69 (36%), 21/54 (41%) and 45/306 (13%) for surgical, radiation and medical oncologists respectively. Most surgeon responders (77%) would feel "uncomfortable making treatment decisions for ILC with a mammogram alone" and 100% would be "more comfortable with an MRI". Although 55% reported treating ILC as they would IDC, 22% of surgeons will purposefully obtain larger gross margins intra-operatively. Some radiation oncologists believe ILC is an independent risk factor for local-regional recurrence after breast conserving surgery (49%), and after mastectomy (29%). 33% of radiation oncologists would offer radiation therapy after mastectomy specifically because of the ILC subtype even in the absence of usual indications for radiotherapy. Most medical oncologists treat ILC comparably to IDC with the factors having the largest influence on systemic treatment decisions being tumour stage, hormone receptor status and HER-2 status. 51% of medical oncologists treat ILC with adjuvant chemotherapy as they do for IDC at least ‘most of the time’, while 40% use neoadjuvant chemotherapy as frequently as they do for IDC at least ‘most of the time’. 75% of medical oncologists manage the hormonal treatment of ILC as they do IDC ‘most of the time’ or ‘always’. Conclusions: There remains significant clinical equipoise in the local-regional and systemic management of ILC. This survey has demonstrated wide variations in both beliefs and practices of management for ILC. Clinical guidance, developed on clinical trials specifically assessing the management of ILC, is required. Citation Format: Carmel Jacobs, Mark Clemons, Mohamed FK Ibrahim, Christina Addison, Jean-Michel Caudrelier, Ian D Graham, Brain Hutton, Angel Arnaout. Oncologist treatment choices in patients with early stage invasive lobular breast carcinoma - a survey [abstract]. In: Proceedings of the Thirty-Seventh Annual CTRC-AACR San Antonio Breast Cancer Symposium: 2014 Dec 9-13; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2015;75(9 Suppl):Abstract nr P5-21-09.
Abstract 3798: Shift of Cx43 isoform expression by PQ1 in SW480 human colorectal cancer cells
Authors: Kristina M. Bigelow, Thu Annelise Nguyen
Journal: Cancer Research (2014)

Abstract Colorectal cancer is one of the most common cancers in men and women in the United States. On average, the risk of developing colorectal cancer is about 1 in 20 over an individuals' lifetime. Cancer cells exhibit many deficiencies in cell-to-cell communication, particularly gap junctional intercellular communication (GJIC). GJIC has been reported to diminish as cancer cells progress. Gap junctions are intercellular channels composed of connexin proteins, which mediate the direct passage of small molecules from one cell to the next. They are involved in the regulation of cell cycle, cell differentiation, and cell signaling. Previously, a class of substituted quinolines, known as PQs, demonstrated to increase GJIC in cancer cells and subsequently attenuated tumor growth. The goal of this study is to determine whether the mode of PQ1 is by directly regulating the connexin protein. The results show that the low expression of connexin 43 (Cx43) in SW480 human colorectal cancer cells remains constant in the presence of PQ1. However, PQ1 caused a change in isoform expression of Cx43 in which there was a shift of Cx43 expression from P0 to P1 and P2 isoforms. This suggests that the trafficking of Cx43 from the Golgi to the membrane occurs in the presence of PQ1. Furthermore, the results show that Cx43 interacts with PKCα in SW480 cells; however, PQ1 does not affect the interaction of Cx43 and PKCα, suggesting that the shift in Cx43 isoforms is not due to PKCα phosphorylation. These findings provide in part that the increase of GJIC by PQ1 is due to the shift in Cx43 from P0 to P1 and P2 isoforms in SW480 colorectal cancer cells. Citation Format: Kristina M. Bigelow, Thu Annelise Nguyen. Shift of Cx43 isoform expression by PQ1 in SW480 human colorectal cancer cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3798. doi:10.1158/1538-7445.AM2014-3798
Recombinant hirudin (lepirudin) for the improvement of thrombolysis with streptokinase in patients with acute myocardial infarction
Authors: Karl-Ludwig Neuhaus, G.Peter Molhoek, Uwe Zeymer, Ulrich Tebbe, Karl Wegscheider, Rolf Schröder, Anne Camez, G.Jan Laarman, Gilles M Grollier, Dirk J.A Lok, Holger Kuckuck, Peter Lazarus
Journal: Journal of the American College of Cardiology (1999)
Abstract 4336: Design of a chamber for studying glucose metabolism by anoxic cancer cells
Authors: Jan Lankelma, Mohamed Ahmed, Adrià López Nadal, Josephine C. Dorsman, Hans Joenje, Hans V. Westerhoff
Journal: Cancer Research (2014)

Abstract Many tumors contain anoxic areas. In vitro studies of anoxia can easily be hampered by contamination with traces of oxygen. In order to find out how temporal depletion of extracellular glucose effects anoxic cell death in vitro we have developed a chamber in which 50 000-500 000 cells can be exposed to approximately 30 μl of medium, resulting in rapid depletion of oxygen and nutrients. Before the experiments, cancer cells were seeded on a glass plate, which was the lower glass plate after clamping with an upper plate that contained an 80-100 μm thick silicone spacer. The medium of the cavity between the two flat glass plates could be flushed through an inlet and outlet hole in the upper plate, located in the corners of the cavity. Samples were collected from the outlet hole for analysis. Most cancer cell lines use both anaerobic and aerobic metabolism to generate ATP, resulting in a more rapid depletion of oxygen than of glucose. We found that after 3 hours the glucose was completely consumed by the MCF-7 human breast cancer cells and that overnight exposure resulted in cell death, except in the immediate proximity of the holes, where oxygen could diffuse into the medium. An interesting observation was that the lactate produced was more than double the glucose used from the medium, suggesting that also intracellular glycogen stores had been consumed by the cancer cells. We are especially interested in using this system to simulate the effect of dietary restriction on individual dormant breast tumors that have not (yet) started angiogenesis. These are hypothesized to contain an outer ring of anoxic cells, that depend on and rapidly consume the lowest concentrations of glucose “at the end of the glucose gradient” from the nearest capillary blood vessel. A temporary decrease of blood glucose may kill these anoxic cells and perhaps affect non-Warburg cells less. The time dependence of cell killing on this decrease will be investigated using this system. Citation Format: Jan Lankelma, Mohamed Ahmed, Adrià López Nadal, Josephine C. Dorsman, Hans Joenje, Hans V. Westerhoff. Design of a chamber for studying glucose metabolism by anoxic cancer cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4336. doi:10.1158/1538-7445.AM2014-4336
A novel non-invasive, in vivo technique for the quantification of leukocyte rolling and extravasation at sites of inflammation in human patients
Authors: Juha Kirveskari, Minna H. Vesaluoma, Jukka A.O. Moilanen, Timo M.T. Tervo, Matthew W. Petroll, Eeva Linnolahti, Risto Renkonen
Journal: Nature Medicine (2001)
Promotional Practices of Pharmacy Benefits Management Companies
Authors: Stuart L. Nightingale
Journal: JAMA (1998)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Infectious Diseases of Humans: Dynamics and Control
Authors: Roy M. Anderson, Robert M. May
Journal: Annals of Internal Medicine (1992)

Part 1 Microparasites: biology of host-microparasite associations the basic model - statics static aspects of eradication and control the basic model - dynamics dynamic aspects of eradication and control beyond the basic model - empirical evidence of inhomogeneous mixing age-related transmission rates genetic heterogeneity social heterogeneity and sexually transmitted diseases spatial and other kinds of heterogeneity endemic infections in developing countries indirectly transmitted microparasites. Part 2 Macroparasites: biology of host-macroparasite associations the basic model - statics the basic model - dynamics acquired immunity heterogeneity within the human community indirectly transmitted helminths experimental epidemiology parasites, genetic variability, and drug resistance the ecology and genetics of host-parasite associations.
Potent activity of the heat shock protein 90 (Hsp90)inhibitor, 17AAG in head and neck squamous cell carcinoma (HNSCC) lines
Authors: Vassiliki A. Papadimitrakopoulou, Stefania Fiorentino, Mohammed Naeemuddin
Journal: Cancer Research (2006)

4700   HNSCC rely on multiple genetic abnormalities that result in several aberrant signaling pathways that in turn mediate cancer maintenance and progression. One of the issues in development of molecular cancer therapeutics is the skepticism regarding the ability of a single drug that targets one of a series of key molecular abnormalities in a particular cancer to deliver clinical benefit. Hsp90 inhibitors appear to be ideal agents by virtue of their property of canceling the oncogenic activity of a number of “client” proteins belonging to multiple key signal transduction pathways promoting tumor proliferation and survival. We have examined the effects of 17AAG a derivative of geldanamycin, in HNSCC by treating twelve HNSCC cell lines and characterizing the sensitivity to the drug in MTT assays characterized the sensitivity to the drug. Among these 12 , 3 cell lines are exquisitively sensitive to the drug with IC 50  in subnanomolar range (FaDu, 22B, MDA 1986), 6 cell lines are sensitive with IC 50  below 100 nM range, and 4 are either modestly sensitive or resistant. Effects on cell cycle by flow cytometry at 24 and 48 hours include mostly an increased proportion of cells in the sub-G0 population and reduced proportion in S phase, while in a few cell lines a G2-M block was noted. These effects were seen at appropriate concentrations even in resistant cells. Apoptotic cell death as measured with annexin V reached levels up to 90% for certain of the sensitive cell lines and up to 50% for resistant. Immunoblot analysis has shown that 17AAG depleted total protein and decreased levels of pAKT and a number of AKT downstream targets, pSTAT3, pSTAT5, pEGFR, pERK1/2, c-Raf, cyclin D1, cdk4 predominantly in a dose-dependent manner but also to a lesser degree a number of other pathways. Study of the effects on cell migration and angiogenesis are currently in progress. These experiments along with combinations with either growth factor or signal transduction inhibitors as well as combination experiments with standard cytotoxics are in progress. Hsp90 inhibitors by virtue of their effects on client proteins can target signal transduction pathways involved in head and neck cancer cell growth and could be used alone or in combination with other signal transduction inhibitors or standard cytotoxics as effective treatment strategies for these malignancies.The information obtained from the present study has led to initiation of animal experiments in an orthotopic tongue cancer model and will hopefully lead to clinical applications in the treatment of HNSCC (supported by P50CA91007-02)
Government should increase benefit levels to allow healthy living, says trust
Authors: Lynn Eaton
Journal: BMJ (2004)

A coalition of 66 UK non-governmental organisations, including the BMA, is calling on the government to review current methods of assessing poverty.

Current methods of determining acceptable rates of benefits and pensions do not reflect the costs of living healthily in the United Kingdom, says the Zacchaeus 2000 Trust, a Christian organisation that, as an umbrella body, includes representatives from Age Concern, Barnardo's, and the National Society for the Prevention of Cruelty to Children.

The government sets minimum levels for benefits without giving adequate weight to the price of goods and services people have to buy, the trust says in a report that was due to be presented …
Regulatory environment for allergen-specific immunotherapy
Authors: S. Kaul, S. May, D. Lüttkopf, S. Vieths
Journal: Allergy (2011)

Products for specific immunotherapy (SIT) are medicinal products according to the European Regulations. To obtain a marketing authorization (MA) within the European Community, the quality, safety and efficacy have to be proven. During the development phase of a medicinal product, applicants have the opportunity to apply for scientific advice by national competent authorities or the European Medicines Agency (EMA) to compile a suitable development plan for the examination of quality and performance of nonclinical and clinical trials. Moreover, a paediatric investigation plan has to be submitted to the Paediatric Committee of the EMA and has to be approved before submission of an application for MA. Several regulatory procedures exist for obtaining a MA in the European Community. The national procedure leads only to marketability in one country whereas the Mutual Recognition, the Decentralized and Centralized Procedures (CP) are intended for MA in several or all member states of the European Union. The CP is mandatory for certain medicinal products, for example for drug substances derived by biotechnological processes such as recombinant allergens. Named Patient Products for SIT are a specialty because they are manufactured on the basis of an individual prescription and marketed without a MA.