tbuckley/cabot-search huggingface dataset:
Abstract 225: Role Of PEDF In Regulation Of Skeletal Blood Flow And Neovascularization In Ischemic Limb Via Interaction With VEGF
Authors: Ken Arima, Yousuke Katsuda, Yutaka Saito, Yoshio Katsuki, Kenichiro Sasaki, Shoichi Yamagishi, Hisao Ikeda, Hisashi Kai, Tsutomu Imaizumi
Journal: Circulation (2007)

[Introduction] We reported that pigment epithelium-derived factor (PEDF) plays an important role in the angiogenesis, vascular permeability and regulation of blood flow in the eye and brain via interaction with VEGF. [Objective] We examined the role of PEDF in ischemia or VEGF-related angiogenesis or regulation of blood flow in severe limb ischemia. [Methods] Unilateral hindlimb ischemia was performed in klotho-mice by binding the unilateral femoral artery. After hindlimb ischemia was surgically induced, we injected saline (control group) or VEGF (3 μg/day × 7, VEGF group) to ischemic limb from day 1 to 7. The expression level of PEDF in the ischemic tissue was examined by RT-PCR method. Furthermore, we injected recombinant mouse PEDF (4 μg/day × 7) or anti-PEDF antibody (60 μg/day × 7) in addition to VEGF (VEGF + PEDF group and VEGF + anti-PEDF group, respectively) to ischemic limb from day 1 to 7. We evaluated limb blood flow by laser doppler blood-flow (LDBF) at day 0, 3, 7, and 14, and number of CD34 positive cells in the ischemic tissue by histology as an index of ischemic angiogenesis at day 7 in all groups. [Results] Expression levels of PEDF in ischemic tissue were significantly elevated in VEGF group. In VEGF group, the ischemic/normal limb blood flow ratio by LDBF analysis and number of CD34 positive cells were significantly increased compared with control group (p &lt; 0.05). The effects of VEGF on blood flow or number of CD34 positive cells were completely abolished by administration of PEDF. Furthermore, anti-PEDF group, accelerated recover of blood flow in ischemic limb, but not number of CD34 positive cells compared in VEGF administration. [Conclusion] VEGF augmented the expression level of PEDF in the ischemic limb. VEGF-induced ischemic angiogenesis was abolished by exogenous administration of PEDF. Furthermore, anti-PEDF antibody augmented VEGF-induced increase of blood flow. These results suggest that PEDF may play an important role in regulation of skeletal blood flow and neovascularization in ischemic limb via interaction with VEGF.
Abstract 18984: Discrepancy Between Originally Assigned and Adjudicated Causes of Out-of-Hospital-Cardiac-Arrest of “No Obvious Cause” in Young Patients
Authors: Katherine S Allan, Laurie J Morrison, Jack V Tu, Arnold Pinter, Matthew R Common, Dhanisha Patel, Brian Nguyen, Cameron Landry, David Dorian, Paul Dorian
Journal: Circulation (2014)

Background: Death due to out-of-hospital cardiac arrest (OHCA) is most commonly attributed to “no obvious cause” after review of the prehospital and in-hospital documentation. To establish cause of death in younger patients, autopsy and toxicology data are important, especially in younger patients. We examined the probable cause of death in a large urban cohort of younger OHCA patients. Methods: A prospective population-based registry of all OHCAs attended by Emergency Medical Services (EMS) in the Toronto area was used to identify cases from 2009-2012. Identified OHCAs were cases aged 2-45 who died and assigned ‘no obvious cause’ based on abstracted data from ambulance call reports and in-hospital chart review, including narrative review and contributing factors compliant with the Resuscitation Outcomes Consortium Epistry Manual of Operations. Cases with inadequate information (coroners and/or EMS), or with expected death (e.g. DNR or long term care) or homicide were then excluded. The remaining cases were adjudicated by 3 reviewers using additional data from coroner investigative statements, autopsy, toxicology and police reports, and classified as primary cardiac or another non-cardiac etiology. Results: A total of 2048 OHCA cases with no obvious cause were identified; of these, 547 were excluded. Of the remaining 1501 cases with complete information, 48.2% were treated by EMS. Of the treated cases, 41.3 % (299/724) of those assigned to ‘no obvious cause’ had an adjudicated cardiac etiology. Of the untreated group, 21.2% (165/777) had an adjudicated cardiac etiology. The majority consisted of drug overdoses (214/425; 50.3%) and other non-cardiac causes (49.7%) including trauma (Figure 1). Conclusions: In younger OHCA patients who die, the category "OHCA of no obvious cause”, on detailed review, is most often due to a non-cardiac cause. This observation may contribute to a better understanding of the etiology of sudden cardiac death in younger patients.
Fenretinide increased ceramides through progressive de novo synthesis and inhibition of sphingomyelin synthesis in a neuroblastoma cell line
Authors: Hongtao Wang, Barry J. Maurer
Journal: Cancer Research (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

4653 

Fenretinide (4-HPR) is a cytotoxic retinoid active in many cancer cell lines in vitro . 4-HPR-induced death occurs in association with an increase of ceramide species (dihydroceramides > ceramides) in many cell lines. Modulators of sphingolipid metabolism can increase 4-HPR-induced cytotoxicity. We reported that 4-HPR increased ceramides by increasing the activities of serine palmitoyltransferase (SPT), the rate-limiting step in de novo ceramide synthesis, and of (dihydro)ceramide synthase. However, recently it was reported that 4-HPR increased ceramides in neuroblastoma cells by sphingomyelin (SM) hydrolysis. Therefore, we further investigated the mechanisms of 4-HPR-induced ceramide(s) increase. METHODS. Sphingomyelin (SM) was pre-labeled using [14C]palmitic acid (PA) followed by washout, labeled continuously during 4-HPR exposure, or pulse-labeled at the end of 4-HPR treatment; de novo synthesized sphingolipids were labeled using [14C]PA or [14C]serine. Sphingolipids were extracted, separated by thin-layer chromatography (TLC), and quantitated by phosphoimager scanning. Cell cytotoxicity was determined using a fluoresence-based assay system (DIMSCAN). RESULTS. In the SK-N-RA neuroblastoma cell line, in cells with pre-labeled SM pools, 4-HPR (10 μM) treatment decreased ceramides by ∼40% over controls at +6 hr (P 0.05). In contrast, 4-HPR treatment increased de novo labeled ceramides (dihydroceramides >> ceramides) by ∼130% over controls at +6 hours. Together, these results suggest that, in SK-N-RA cells, 4-HPR increased ceramides mainly through de novo synthesis but with a contribution from inhibition of SM formation.To further investigate the role of sphingomyelin metabolism in 4-HPR-treated cells, D609, a SM synthase inhibitor, was employed. A 24 hr treatment of D609 (375 μM) inhibited SM formation by 65% (P<0.001), increased total ceramides by ∼60% (P=0.003), and, in combination with 4-HPR, D609 may have increased total ceramides a further 20% compared to 4-HPR-only treated cells (P=0.13). Further, D609 synergized 4-HPR cytotoxicity (4-HPR-alone, LD99 = ∼9.5 μM; D609+4-HPR, LD99 = 6.5 μM 4-HPR; survival fraction = 0.08 vs 0.0007 at 9 μM 4-HPR, C.I. < 0.4). CONCLUSIONS. In SK-N-RA neuroblastoma cells, 4-HPR increased ceramides primarily by de novo synthesis with a contribution from inhibition of SM synthesis. Further, inhibition of SM formation may constitute a novel method of synergizing 4-HPR cytotoxicity for neuroblastoma treatment.
Short-term cold acclimation improves insulin sensitivity in patients with type 2 diabetes mellitus
Authors: Mark J W Hanssen, Joris Hoeks, Boudewijn Brans, Anouk A J J van der Lans, Gert Schaart, José J van den Driessche, Johanna A Jörgensen, Mark V Boekschoten, Matthijs K C Hesselink, Bas Havekes, Sander Kersten, Felix M Mottaghy, Wouter D van Marken Lichtenbelt, Patrick Schrauwen
Journal: Nature Medicine (2015)
Abstract 15071: AMR101 Lowers Triglycerides, Atherogenic Lipoprotein, Phospholipase A2, and High-sensitivity C-reactive Protein Levels in Patients With High Triglycerides and on Background Statin Therapy (the ANCHOR Study)
Authors: Christie M Ballantyne, Harold E Bays, John J Kastelein, Evan A Stein, Jonathan Isaacsohn, Rene A Braeckman, Paresh N Soni
Journal: Circulation (2011)

Objective: AMR101 is an omega-3 agent containing ≥96% pure icosapent ethyl (ethyl-eicosapentaenoic acid [EPA], the ethyl ester of EPA). The efficacy and safety of AMR101 were evaluated in a phase 3...
Abstract 11266: Prognostic Implications of Normal Stress Echocardiogram in Patients With Angiographically Significant Coronary Artery Disease
Authors: Siu-Sun Yao, Omar Wever-Pinzon, Sripal Bangalore, Daniel Pudpud, Farooq A Chaudhry
Journal: Circulation (2010)

Background: This purpose of this study was to examine the prognostic implications of normal stress echocardiography in patients with angiographically significant coronary artery disease (CAD). Meth...
Abstract 3829: Twelve Years' Experience of Aortic Stent Graft Implantation: Mortality and Clinical Outcome
Authors: Chul Min Ahn, Young-Guk Ko, Donghoon Choi, Yangsoo Jang, Do Yun Lee, Byung-Chul Chang, Won-Heum Shim
Journal: Circulation (2006)

Background: Endoluminal stent-graft repair for aortic aneurysm or dissection replaced some part of surgical correction because of its higher success rate with less invasiveness and mortality. Aortic stent graft was performed at the various aortoiliac pathology for the last 12 years. The object of study is to assess long-term mortality and clinical results of the endoluminal therapy for patients with aortoiliac pathology.  Methods: A retrospective analysis was done on 166 pts (62.7±12.0) who underwent stent-graft repair at our single center from September, 1994 to April, 2006. The indications were progression of aortic pathology despite adequate medical treatment, dynamic obstruction, aggravation of pain, or other medically intractable complications in either acute or chronic presentation.  Results: Median follow-up duration was 3.8 years (2 mon~12 yrs, 3.8 ± 3.2 yrs). Clinical diagnoses were aortic aneurysm (109 pts, 57.7%), aortic dissection (40 pts, 21.2%), ruptured aorta (6 pts, 2.6%), and others (11 pts, 5.8%). Locations of each lesion were abdominoiliac vessel (32.8%), thoracic aorta (28.6%), abdominal aorta (21.2%), and others (5.2%). Angiographic success, defined as immediate obliteration of target lesion without major endoleak or complete exclusion of aneurysm, was obtained in 101/109 (92.7%) of aortic aneurysm, and 39/40 (97.5%) of aortic dissection. Clinical success, defined as complete obliteration of the target lesion at follow-up without further interventional or operative management, was achieved in 89/109 (81.7%) of aortic aneurysm, and 34/40 (85%) of aortic dissection. Among these patients, 15 pts were expired due to multiorgan failure (3.2%), heart failure (3.2%), and renal failure (1.1%). Co-morbidities include coronary artery disease requiring intervention (16.4%), connective tissue disease including Marfan syndrome or Behcet disease (2.1%), and others (1%).  Conclusions: The outcome of endovascular stent-graft repair of aortic disease was acceptable with a clinical success rate of 74.1% without acute procedure-associated mortality. Endolu-minal stent-graft repair should be considered as a safe and effective alternative treatment option of aortic pathology particularly in pts with a high risk of surgical mortality.
Measles imported by returning U.S. travelers aged 6-23 months, 2001-2011.
Authors: N. M. Cocoros, J. Zipprich, D. Kuhles, E. Rausch-Phung, C. R. Schulte, D. S. Blog, P. Lurie, R. Wiseman, C. Kroll, C. DeBolt, P. K. Kutty, S. B. Redd, A. E. Barskey, J. S. Rota, P. A. Rota, G. L. Armstrong, W. J. Bellini, K. M. Gallagher, A. S. Mahamud
Journal: MMWR Morbidity and Mortality Weekly Report (2011)
Validation of clinical prognostic factors for survival in 1,043 Japanese patients with metastatic renal cell carcinoma (mRCC)
Authors: Y. Tomita, S. Naito, S. Ozono, T. Terachi, S. Naito
Journal: Journal of Clinical Oncology (2006)

20075 Background: Increasing number of patients and death of RCC has been reported in many countries including US and Japan. For choosing optimal treatment, determining risk of patients is essential. Although several prognostic factors of mRCC has been investigated, it was based on data of Caucasian. Since racial difference in biological character of cancer has been identified in several types of cancer, we validated the relationship between reported prognostic factors and survival in patients with mRCC in Japanese patients. Methods: We reviewed the medical records of 1,043 patients who had mRCC in the period to follow up, or at the first visit examination between 1988 and 2001 of 26 center in Japan. Results: Average survival of the 708 patients who died for renal cell carcinoma was 20.84 months. On the other hand, histological high grade (&gt; WHO grade 2), metastasis at the first visit examination, short period from the first visit to recognition of metastasis (within 12 months), brain, bone or, liver metastasis, a number of metastatic sites (&gt;1), high performance status (ECOG PS &gt;0), high corrected serum calcium, low hemoglobin, and high CRP were prognostic factors for survival. However, LDH were not related. The median time to death with zero risk factor was 38.5 months, but those with &gt;3 risk factors was 10 months. The patients with &gt;4 risk factors was 4 months. Conclusions: Almost all prognostic factors reported in previous studies mainly based on Caucasian data had same impact on Japanese patients with mRCC. However, elevation of LDH did not have relationship to prognosis. Moreover, the time to death from diagnosis of mRCC in patients with poor prognostic factors was likely shorter compared with other studies. These results suggest Japanese with mRCC might have different character from those in US or European countries though further study in patient’s and tumor characteristics is warranted. No significant financial relationships to disclose.
R-Mini-CHOP Versus R-Bendamustine As Primary Treatment for Diffuse Large B-Cell Lymphoma (DLBCL) in Elderly and Comorbid Patients
Authors: Jakob Hammersen, Maria Madeleine Rüthrich, Martina Kleber, Ulrich Wedding, Michael Sommer, Daniela Müller, Peter Schlattmann, Andreas Hochhaus, Paul La Rosee
Journal: Blood (2014)
Antitumor reactivity of T cells generated from lymph nodes draining an SEA-expressing murine B16 melanoma and secondarily activated with dendritic cells.
Authors: Jiyun Yu, Rong Tian, Xing Chen, Shaohui Cheng, Alfred E. Chang, Qiao Li
Journal: Cancer Research (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

5575 

Tumor-draining lymph nodes (TDLN) have been used as a source of antitumor effector cells for adoptive immunotherapy of cancer. The successful use of this strategy depends largely on the immunogenicity of the tumor drained by the lymph nodes as well as the protocols for the secondary in vitro T cell activation and expansion. We constructed a eukaryotic expression vector pcDNA3.1-SEA (staphylococcal enterotoxin A) and transfected it into the B16 murine melanoma tumor cells by means of liposome-mediated transfection. We then used SEA-modified B16 cells to induce the TDLN (SEA TDLN) in syngeneic hosts. These TDLN cells were activated in vitro using anti-CD3/anti-CD28/IL-2 with or without dendritic cells (DC) pulsed with B16 tumor lysates. Wild-type (Wt) TDLN induced by using non-modified B16 tumor was used as a control. In vitro analyses showed that SEA TDLN cells proliferated more vigorously than Wt TDLN in response to anti-CD3/anti-CD28/IL-2 stimulation. Tumor lysate-pulsed DC (TPDC) significantly enhanced the proliferation of both Wt TDLN and SEA TDLN cells (p<0.05). Functionally, SEA TDLN cells activated with anti-CD3/anti-CD28/IL-2 in the presence of TPDC demonstrated the highest CTL activity and secreted the highest levels of IFNγ towards tumor cells compared with any other experimental group. Using the metastatic pulmonary tumor model, we adoptively transferred (i.v.) Wt TDLN and SEA TDLN cells sensitized in vivo respectively by B16 or SEA-B16 tumor, and activated in vitro by anti-CD3/anti-CD28/IL-2 with or without TPDC. SEA TDLN cells mediated tumor regression more effectively than Wt TDLN cells activated with anti-CD3/anti-CD28/IL-2. The addition of TPDC in culture significantly augmented the therapeutic efficacy of both Wt TDLN and SEA TDLN cells (p<0.05), with SEA-TDLN cells activated with anti-CD3/anti-CD28/IL-2 plus TPDC being the most potent effector cells in mediating tumor eradication. These results thus indicate that enforced expression of superantigen SEA in a poorly immunogenic tumor, B16, can enhance the immunogenicity of the tumor in vivo . They also support the use of autologous tumor lysate-pulsed DC during the secondary in vitro activation/expansion procedure in order to generate more potent tumor specific effector cells for adoptive immunotherapy.
Coronary Care
Authors: INGEMAR CULLHED
Journal: Annals of Internal Medicine (1975)

Comments and Correction1 October 1975Coronary CareINGEMAR CULLHED, M.D., BJÖRN SMEDBY, M.D., URBAN WAERN, M.D.INGEMAR CULLHED, M.D.Search for more papers by this author, BJÖRN SMEDBY, M.D.Search for more papers by this author, URBAN WAERN, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-83-4-575_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: It is difficult to compare input and output data on hospital care. Such a "cost-effectiveness" study concerning coronary care has been done by Martin and associates (1). Their conclusion was that, despite considerable increase in input, there was no increase in output measured as duration of hospitalization and hospital mortality.Their study refers, however, to a precoronary period. Further, the number of patients was small, 37 to 53 patients per studied year. Their conclusions may not be representative.It is well known that hospital mortality in myocardial infarction has decreased by about 10% since the introduction of...References1. MARTIN S, DONALDSON M, and LONDON C: Inputs into coronary care during 30 years. Ann Intern Med 81:289-293, 1974 LinkGoogle Scholar2. MATHER H, PEARSON N, and READ K: Acute myocardial infarction: home and hospital treatment. Br Med J 3:334-338, 1971 CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: INGEMAR CULLHED, M.D.; BJÖRN SMEDBY, M.D.; URBAN WAERN, M.D.Affiliations: Department of Cardiology Medical Clinic Department of Community Medicine University Hospital Uppsala Sweden PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 October 1975Volume 83, Issue 4Page: 575-575KeywordsHospitalizationsMortalityMyocardial infarction ePublished: 1 December 2008 Issue Published: 1 October 1975 PDF downloadLoading ...
Generic Imatinib in a Patients with Chronic Myeloid Leukemia in Chronic Phase- a Single Centre Experience
Authors: Ivana M Urosevic, Marina Dokic, Ivanka Savic, Aleksandar Savic, Ivana Milosevic, Nebojsa Rajic, Nebojsa V. Andjelkovic, Borivoj Sekulic, Nada V Vlaisavljevic
Journal: Blood (2014)
Abstract 1700: miRNA-dependent Regulation of Smooth Muscle Cell Function During Neointima Formation
Authors: Wiebke Bielenberg, Andre Prock, Stieger Philipp, Harald Tillmanns, Daniel Sedding
Journal: Circulation (2008)

Introduction: Micro RNAs (miRNAs) are implicated to regulate mRNA levels of up to 30% of mammalian genes, comprising key regulators for cellular function. Maturation and expression of miRNAs is tig...
Abstract 12303: Physician Staffed Emergency Helicopter Transport of Patients With ST Elevation Myocardial Infarction Improves Outcomes Compared to Helicopter Transport Without Physicians
Authors: Amish N Raval, Joseph Mitchell, Brenda Larson, Zhanhai Li, S. Michael Gharacholou
Journal: Circulation (2013)

Objective:  Helicopter Emergency Medical Services (HEMS) commonly transport patients with ST Elevation Myocardial Infarction (STEMI) to hospitals that can perform timely percutaneous coronary intervention. HEMS typically consist of paramedics and flight nurses who have advanced cardiac life support skills; however, a number of services also employ Emergency Medicine physicians. Physician-staffed crews may offer enhanced emergency care at the scene and during transport; however, the impact of physician-staffed HEMS on patient outcomes for STEMI is unknown. We hypothesized that physician staffed HEMS for STEMI transport improves patient outcomes compared to non-physician staffed HEMS.   Methods:  U. Wisconsin Hospital & Clinics receives STEMI patients via physician and non-physician staffed HEMS. Demographic, in-transport and in-hospital data was retrospectively collected from the University of Wisconsin Hospital and Clinics contribution to the National Cardiovascular Data Registry (NCDR), electronic medical record and HEMS flight record between August 2006 and June 2012. In-transport and in-hospital adverse events including death, stroke, cardiac arrest, major arrhythmia, respiratory failure requiring advanced airway, hypotension and recurrent MI were recorded. The primary endpoint was defined as the composite of mortality and/or NCDR defined adverse events.   Results:  327 subjects transported by physician HEMS and 23 by non-physician HEMS in the study period. Age, sex, Killip Class, infarct location were similar between groups. Median flight distance, regional door to STEMI activation, and in-transport adverse events were also similar between groups. The primary endpoint was significantly improved for the physician HEMS group vs. the non-physician HEMS group (19% vs. 43% respectively, p=0.013). Serious arrhythmias were also less frequent (3% vs. 13%, p=0.037). Mortality was lower with physician HEMS vs. non-physician HEMS, but this did not reach statistical significance (5% versus 9%; p=0.33).   Conclusion:  In this retrospective, single center study, subjects with STEMI who were transported via physician staffed HEMS appeared to have improved in-hospital outcomes compared to those transported by non-physician staffed HEMS.
Ross River virus disease in two Dutch travellers returning from Australia, February to April 2015
Authors: C Reusken, N Cleton, M Medonça Melo, C Visser, C GeurtsvanKessel, P Bloembergen, M Koopmans, J Schmidt-Chanasit, P van Genderen
Journal: Eurosurveillance (2015)

We report two cases of Ross River virus (RRV) infection in Dutch travellers who visited Australia during February to April 2015. These cases coincided with the largest recorded outbreak of RRV disease in Australia since 1996. This report serves to create awareness among physicians to consider travel-related RRV disease in differential diagnosis of patients with fever, arthralgia and/or rash returning from the South Pacific area, and to promote awareness among professionals advising travellers to this region.
Feasibility, Accuracy, and Reproducibility of Real-Time Full-Volume 3D Transthoracic Echocardiography to Measure LV Volumes and Systolic Function
Authors: Paaladinesh Thavendiranathan, Shizhen Liu, David Verhaert, Anna Calleja, Adrien Nitinunu, Thomas Van Houten, Nathalie De Michelis, Orlando Simonetti, Sanjay Rajagopalan, Thomas Ryan, Mani A. Vannan
Journal: JACC. Cardiovascular imaging (2012)