tbuckley/cabot-search huggingface dataset:
Abstract 1360: DNA methylation analysis in self-sampled material as a triage test in hrHPV positive women
Authors: Aniek Boers, Remko P. Bosgraaf, Roland W. van Leeuwen, Ed Schuuring, Leon FAG Massuger, Johan Bulten, Willem J. Melchers, Ate GJ van der Zee, Ruud L. Bekkers, Bea Wisman
Journal: Cancer Research (2014)

Abstract Background: Primary hrHPV testing in cervical cancer screening shows relatively low specificity. To decrease the number of unnecessary referrals for colposcopy, triage testing is necessary. In this study, DNA methylation analysis was compared with routine cytology for triage testing in hrHPV positive women. Moreover, feasibility of DNA methylation analysis directly on original brush-based self-sampled specimens was assessed. Methods: Non-responding women from population-based screening were invited to self-collect a cervico-vaginal specimen for hrHPV testing; hrHPV positive women were referred to a physician for liquid-based sampling. For this pilot study, DNA methylation analysis was performed on 128 triage samples and on 50 matched Evalyn brush self-samples with QMSP for C13ORF18, EPB41L3, JAM3 and TERT. Results: In the physician-taken triage material, DNA methylation analysis of JAM3 showed the highest specificity (88%) combined with a high sensitivity (82%) for detection of CIN3+, whereas cytology showed a specificity of 48% and a sensitivity of 91%. Out of 39 women with abnormal cytology and normal histology (false positive by cytology), 87% were negative for JAM3 and 90% for C13ORF18 methylation. The agreement for DNA methylation analysis performed directly on the matched self-sampled material and the physician-taken samples was 90% for C13ORF18 (κ=0.77; p&amp;lt;0.001) and 88% for JAM3 (κ=0.75, p&amp;lt;0.001). Conclusion: DNA methylation analysis as a triage test in hrHPV positive women outperformed cytology due to higher specificity without losing its sensitivity and is also feasible to be performed directly on Evalyn Brush self-samplers. This might result in more correct referrals to the gynecologist and eliminate the need for a new scraping taken by a medical professional thereby offering great logistic and financial advantages. This study was financed by the Dutch Cancer Society (RUG-NKB2009-4577). Citation Format: Aniek Boers, Remko P. Bosgraaf, Roland W. van Leeuwen, Ed Schuuring, Leon FAG Massuger, Johan Bulten, Willem JG Melchers, Ate GJ van der Zee, Ruud LM Bekkers, Bea Wisman. DNA methylation analysis in self-sampled material as a triage test in hrHPV positive women. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1360. doi:10.1158/1538-7445.AM2014-1360
A Longitudinal Study of Hormonal Reactions Accompanying Life Events in Recently Resettled Refugees
Authors: Hans Peter Söndergaard, Töres Theorell
Journal: Psychotherapy and Psychosomatics (2002)

&lt;i&gt;Background:&lt;/i&gt; Refugees constitute a growing section of the general population in many countries. It is therefore important to study which factors in everyday life are important to recently resettled refugees after they have been granted residence. &lt;i&gt;Methods:&lt;/i&gt; Life events from a checklist, as well as categories derived from written responses to open-ended questions were analysed (repeated-measures ANOVA). Changes over time in mean serum/plasma concentrations of cortisol, thyroxine, prolactin, and dehydroepiandrosterone sulphate (DHEA-S) were compared in subjects with and without the reported events. &lt;i&gt;Results:&lt;/i&gt; Distress in significant others (close friends or first-degree relatives) and a perception of excessive demands in everyday life were associated with increases in serum cortisol. Events associated with decreased levels of prolactin were typically situations of strain in relation to authorities or individuals to which the subjects were in a position of dependency. DHEA-S changed in opposite directions in post-traumatic stress disorder (PTSD) and non-PTSD subjects. DHEA-S also changed with positive events. &lt;i&gt;Conclusions:&lt;/i&gt; Distress in significant others and a too demanding everyday life lead to significant changes in the stress-responsive hormones cortisol, prolactin and DHEA-S. Since DHEA-S behaves differently in PTSD, this condition is a potential confounder in studies of DHEA-S with an unknown proportion of PTSD among participants.
Novel glycidyl methacrylated dextran (Dex-GMA)/gelatin hydrogel scaffolds containing microspheres loaded with bone morphogenetic proteins: Formulation and characteristics
Authors: Fa-Ming Chen, Yi-Min Zhao, Hai-Hua Sun, Tao Jin, Qin-Tao Wang, Wei Zhou, Zhi-Fen Wu, Yan Jin
Journal: Journal of Controlled Release (2006)
CHEMOTHERAPY OF TRYPANOSOME AND SPIROCHETE INFECTIONS
Authors: Wade H. Brown, Louise Pearce
Journal: The Journal of Experimental Medicine (1919)

The essential facts to be gathered from these studies of the toxicologic action of N-phenylglycineamide-p-arsonic acid may be summarized very briefly. The substance is one which lends itself well to almost any method of administration and can be given to animals in very large doses. The tolerance of different animal species varies rather widely but with one exception the reaction of laboratory animals to toxic doses of the drug is of favorable character. That is, toxic effects are confined to doses relatively close to the minimum lethal dose and the recovery of animals from sublethal intoxications is remarkably rapid and complete. This feature of the action of the drug makes possible the repeated administration of even very large doses at comparatively short intervals of time without incurring the dangers incident to cumulative action or to superposition of toxic effects. On the contrary, by taking advantage of this peculiarity of action, it is possible to develop such a degree of tolerance on the part of animals that the dose of the drug administered can be progressively increased to a point well above that which is fatal to the normal animal, and this stands out as the feature of the toxicologic action of N-phenylglycineamide-p-arsonic acid which is of greatest significance in the use of the drug for therapeutic purposes.
Hepatocyte growth factor receptor and the c-met oncogene Bottaro DP, Rubin JS, Faletto DL, Chan AM-L, Kmiecik TE, Vande Woude GF, Aaronson SA. Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science 1991;251:802?804
Authors: N FAUSTO
Journal: Hepatology (1991)
THE RELATION OF MONOCYTES AND CLASMATOCYTES TO EARLY INFECTION IN RABBITS WITH BOVINE TUBERCLE BACILLI
Authors: Florence R. Sabin, Charles A. Doan
Journal: The Journal of Experimental Medicine (1927)

1. The early reaction to intravenous tubercular infection in the various organs of the rabbit reveals a pathognomonic response in the lungs within 24 hours; the specific response in the liver, spleen, lymph glands, and bone marrow, follows from the 6th to the 14th days. 2. The development and extent of the pathologic process has been analyzed in terms of the activity of monocytes and clasmatocytes. 3. The criteria for differentiating these mononuclear phagocytic cells into two strains have been analyzed and the technics discussed. 4. The clasmatocyte phagocytizes tubercle bacilli freely and fragments them, as it does all cellular and other debris. 5. The monocyte stimulated to metamorphose into the typical epithelioid and giant cell of the Langhans type retains the tubercle bacilli intact, with power to survive and multiply, over long periods of time. 6. The normal number of monocytes or the degree to which monoblasts may be stimulated to development and maturation, together with the activity of the clasmatocytes in destroying bacilli, in any particular region, would appear to be a function of the rapidity and extent of the local tubercular involvement.
658. Development and Evaluation of an Infusion Device for AAV-Mediated Gene Delivery to the Brain
Authors: Jurg M. Sommer, Laura M. Sanftner, Haihwa Kao, Peter H. Smith, Brian M. Suzuki, Joseph Vargas, John R. Forsayeth, Janet Cunningham, Krys S. Bankiewicz, Jan Bernal, Ruth Lessard, Glenn Pierce, Kirk W. Johnson
Journal: Molecular Therapy (2005)

A custom infusion device was developed for delivery of recombinant AAV2 to human putamen in a phase I/II clinical trial for treatment of Parkinson's disease. The device was designed to 1) minimize vector loss to the internal surfaces of the cannula and tubing, 2) minimize the hold-up volume of the device such that vector could be dispensed efficiently from a syringe placed approximately four feet away from the site of infusion, 3) attach to a commonly used stereotactic surgical frame, 4) connect to a programmable syringe pump, and 5) allow manufacturing in compliance with quality system requirements (QSR) for medical devices.
Infantile Quadriceps-Femoris Contracture Resulting from Intramuscular Injections
Authors: Margaret G. Norman, Anthony R. Temple, Jerome V. Murphy
Journal: New England Journal of Medicine (1970)

THE following case is described to alert those caring for infants to the possibility that repeated intramuscular injections into the thighs of infants may cause clinically serious contractures of the quadriceps femoris.1 Case ReportA male infant (M.G.H. 150–80–53) was transferred to the Massachusetts General Hospital shortly after birth for surgical repair of a large ruptured omphalocele. He was treated with intramuscular oxacillin, kanamycin and nafcillin. He received 4 intramuscular thigh injections every day for the first 35 days of life. The volume of antibiotic solution ranged from 0.08 to 0.42 ml per injection.When he was 41 days old, . . .
BLOOD CALCIUM AND INORGANIC PHOSPHATES IN CHILDREN WITH MARKED LACK OF MUSCLE TONE
Authors: L. Wills
Journal: BMJ (1925)

additioni of cod-liver oil.He also claims that the muscular flabbiness of rickets can be cured by the same line of treat- ment.He believes that the cereals have a neutralizing actioIn oii the antirachitic factor, so that on high cereal (liets the dose of cod-liver oil must be increased.Vining3 also believes in a dietetic error, probably carbohydrate excess, as one of the causes of the " debilitated state," cItltough he considers tlle toxic factor to be more important.Ai inivestigation was made of the diets of the children attendinig tlle out-patient department, and it was found that as a genieral rule they were all on the same badly balanced diet, wllich was too liberal in calories for the output of energy in the day.This diet was ridiculously hiigh in carbolhydrate, whiclh was mainly cereal, and low in animiial proteins and fats.Although all the children of the poor were on this badly balanced diet they did not all show flabbiness, and some had remarkably good tone.This difference was seen to occur even in the same family.Fuurther, many of these flabby children were treated for moniths on therapeutic doses of cod-liver oil with no improvemenit, but at the same time it must be noted that the cereals were not restricted, as such a measure was impracticable when dealing with the out-patient class of patients.It is obvious from the above that other factors besides diet must be conicernied in the production of muscular hypotonia.It is interesting in this connexion to note the reports of Holt anid Fales4 on children in an institution in New York.Tlese children received a most liberal diet, but one whicl included a large excess of carbohydrate (over 60 per cent.),anid yet tlley were in most excellent physical condition.Pr'actically the same portion of food was allowed to both the youniger and older children, but both increased niormally ill Weight and remiiainied in good health.CONFINEMENT AND LACK OF EXERcISE.That rickets, and especially lack of muscle tone, is due to coinfinemiient lhas been the opinion of the Glasgow school for many years.Recently Galbraith5 stated that althouglh
Nutrition and Hypertension
Authors: HARRIET P. DUSTAN
Journal: Annals of Internal Medicine (1983)

The supplement that is Part 2 of this issue (1) carries the proceedings of a conference held in Washington, D. C, last September on nutritional factors in hypertension. It is a valuable and contemporary document. The growing concern that nutritional factors may cause high blood pressure is relatively new. Only in the last 10 years has the prevention of hypertension been considered seriously, and we still know relatively little about the factors that initiate hypertension. Interest has focused on food because epidemiologic evidence shows marked dietary differences between populations with differing prevalences of hypertension, and because change in dietary lifestyles
Sex Chromatin Determination From Hair
Authors: Joe C. Culbertson
Journal: JAMA (1969)

To the Editor:—  The study of the sex chromatin pattern has become an important diagnostic tool in clinical medicine. A technique recently introduced1for obtaining this information in a rapid, simple manner is evaluated.  The method uses the cells of the root sheath of a capital hair. The hair is pulled, stained with freshly filtered aceto-orcein for approximately 30 seconds at 65 C and inspected under a magnifying glass to show the structures (Fig 1,A). With the blade of a scalpel, the bulb is removed (Fig 1,B) and the root sheath (a cylinder of cells) slipped off the shaft (Fig 1,C). With practice the cylindrical sheath can be obtained without fibrous material. The root sheath is stained with aceto-orcein to impart clarity to the nuclear structures. This material is compressed under a coverslip to obtain a monolayer of cells. In a suitable area, hundreds of cell
The Nation's Changing Blood Supply System
Authors: Jeffrey McCullough
Journal: JAMA (1993)

DURING the past several years, great interest has developed in the safety of the blood supply in the United States and the manner in which our nation's blood supply is obtained and distributed. Today, this country's blood bank system finds itself in a changed regulatory environment; under considerable public scrutiny; carrying out a much larger, more complex work load; in a more litigious environment; with an organizational infrastructure in need of strengthening at a time of increasing cost constraints; and viewed by some as having inadequately responded to the issues of the 1980s. The manner and effectiveness with which the present issues in blood banking are resolved will have a major impact on the system used to obtain the nation's blood supply and the safety, adequacy, and cost of that supply. This article examines the present issues of concern in the US blood banking system and describes how the outcome
Surgery of the Alimentary Tract
Authors: Earle W. Wilkins
Journal: JAMA (1978)

This first volume of<i>Surgery of the Alimentary Tract</i>, listed as second edition, is in reality a new text. In contrast to the original three-volume work copyrighted in 1955, this edition devotes the entire opening volume (excepting the final chapter on tracheostomy) to consideration of the esophagus. Spending nine years in the making (from the time of invitation for revision), Dr Shackelford records in his preface that "there have been so many new ideas and developments in esophageal surgery that the volume had to be rewritten rather than revised." He acknowledges liberal assistance in expertise from Drs David Skinner, Richard Kriffer, Jr, F. G. Pearson, and many others. It is obviously a collaborative effort, almost monumental in scope. The volume consists of 13 precisely drawn topics from "Anatomy and Physiology" to "Excisional Operations." It is thus nicely structured and represents the most thorough treatise on this most difficult organ since
Angiographic ‘haziness’ following stent deployment: Differential diagnosis using intravascular ultrasound
Authors: Khaled M. Ziada, E.Murat Tuzcu, Anthony C. De Franco, William A. Magyar, Thomas B. Ivanc, Patrick L. Whitlow, Russell E. Raymond, Stephen G. Ellis, Steven E. Nissen
Journal: Journal of the American College of Cardiology (1996)
Risky Business: Genetic Testing and Exclusionary Practices in the Hazardous Workplace
Authors: Mark A. Rothstein
Journal: JAMA (1992)

Since 1990 the debate about medical screening of workers has been driven largely by four developments that reflect the scientific, political, economic, and legal dimensions of the issue. First, the Human Genome Project, officially started in 1990, is a 15-year, $3-billion program to map and sequence all of the estimated 100 000 human genes. As a result of this initiative, geneticists will be able to assess an ever-widening range of human genetic factors, which will include identifying individuals who are presymptomatic for late-onset single gene disorders, carriers of recessive and X-linked traits, and persons at increased risk for multifactorial disorders. This information will relate primarily to nonoccupationally related disorders, but it still will be of great interest to employers under pressure to reduce the escalating costs of employee health and benefits programs. Second, because our political system has failed to address the inherent conflict raised by a health insurance system
Increased levels of circulating Helios+ FoxP3+ natural regulatory T cells in systemic lupus erythematosus
Authors: T. Alexander, L. Templin, S. Kohler, C. Gross, A. Sattler, A. Meisel, C. Perka, G.-R. Burmester, R. Arnold, A. Radbruch, A. Thiel, F. Hiepe
Journal: Annals of the Rheumatic Diseases (2011)

<h3>Objectives</h3> The authors aimed to analyse the coexpression level of Helios, a member of the Ikaros transcription factor family, in FoxP3<sup>+</sup> regulatory T cell (Treg) subsets to characterise the pool of circulating natural Tregs in patients with systemic lupus erythematosus (SLE). <h3>Methods</h3> Multicolour flow cytometry was performed to analyse the coexpression of Helios, CD25, CD45RA and CD31 in FoxP3<sup>+</sup> Tregs from peripheral blood of 20 patients with SLE, 20 age- and sex-matched healthy controls (HC), 6 patients after thymectomy for myasthenia gravis and 6 patients after receiving immunoablation and autologous stem cell transplantation (ASCT) for SLE. Statistical analyses were performed using the paired t-test. <h3>Findings</h3> The level of circulating FoxP3<sup>+</sup> T cells among CD4 T cells was significantly higher in SLE compared to HC (mean 14.5 vs 7.2%, p=0.002) with Helios being coexpressed at significantly higher levels in SLE (81.4 vs 69.0%, p=&lt;0.0001). With respect to CD45RA coexpression, CD4<sup>+</sup> FoxP3<sup>+</sup> T cells can be subdivided into three distinct subpopulations, all of which are present at significantly higher levels among CD4 T cells in SLE: CD45RA<sup>+</sup> FoxP3lo resting Tregs (p=0.01), CD45RA<sup>−</sup> FoxP3hi activated Tregs (p=0.01) and CD45RA<sup>−</sup> FoxP3lo non-Treg cells (p=0.004). In SLE, Helios expression levels were significantly higher in all of these subsets compared to HC: 90.7 versus 85.1% in CD45RA<sup>+</sup> FoxP3lo Tregs (p=0.003), 83.3 versus 74.7% in CD45RA<sup>−</sup> FoxP3hi Tregs (p=0.003) and 73.8 versus 52.5% in CD45RA<sup>−</sup> FoxP3lo non-Treg cells (p&lt;0.0001). Within the CD45RA<sup>+</sup> FoxP3lo Treg subset, the coexpression level of CD31 was significantly lower in SLE (p=0.03) and patients after thymectomy (p=0.001), but higher in patients after ASCT who developed a thymic reactivation (p=0.04). <h3>Conclusion</h3> The authors9 data are the first to demonstrate that circulating Helios<sup>+</sup> FoxP3<sup>+</sup> natural Treg levels are significantly increased in SLE patients compared to healthy controls. This does, however, not necessary reflect an increased thymic output of natural Tregs in SLE. The decreased coexpression levels of CD31, as a surrogate marker for recent thymic emigrants, found among CD45RA<sup>+</sup> FoxP3<sup>+</sup> Tregs in SLE rather suggests that the thymic output is diminished in SLE implicating that Treg levels are primarily maintained through peripheral expansion of Helios<sup>+</sup> FoxP3<sup>+</sup> Tregs in SLE. The level of Helios<sup>−</sup> FoxP3<sup>+</sup> induced Tregs are, on the other hand, decreased in SLE for so far unknown reasons.
Early recurrences in histologically benign/grade I meningiomas are associated with large tumors and coexistence of monosomy 14 and del(1p36) in the ancestral tumor cell clone
Authors: Angel Maillo, Alberto Orfao, Ana B. Espinosa, José María Sayagués, Marta Merino, Pablo Sousa, Monica Lara, María Dolores Tabernero
Journal: Neuro-Oncology (2007)

Tumor recurrence is the major clinical complication in meningiomas, and its prediction in histologically benign/grade I tumors remains a challenge. In this study, we analyzed the prognostic value of specific chromosomal abnormalities and the genetic heterogeneity of the tumor, together with other clinicobiological disease features, for predicting early relapses in histologically benign/grade I meningiomas. A total of 149 consecutive histologically benign/grade I meningiomas in patients who underwent complete tumor resection were prospectively analyzed. Using interphase fluorescence in situ hybridization, we studied the prognostic impact of the abnormalities detected for 11 different chromosomes, together with other relevant clinicobiological and histopathological characteristics of the disease, on recurrence-free survival (RFS) at 2.5, 5, and 10 years. From the prognostic point of view, losses of chromosomes 9, 10, 14, and 18 and del(1p36) were associated with a shorter RFS at 2.5, 5, and 10 years. Similarly, histologically benign/grade I meningiomas showing coexistence of monosomy 14 and del(1p36) in the ancestral tumor cell clone displayed a higher frequency of early relapses. In fact, coexistence of -14 and del(1p36) in the ancestral tumor cell clone, together with tumor size, represented the best combination of independent prognostic factors for the identification of those patients with a high risk of an early relapse. Our results indicate that patients with large histologically benign/grade I meningiomas carrying monosomy 14 and del(1p36) in their ancestral tumor cell clone have a high probability of relapsing early after diagnostic surgery. These findings suggest the need for closer follow-up in this small group of patients.
Do It to Yourself Section: Surgery
Authors: LOREN Pankratz
Journal: JAMA (1986)

To the Editor.—  In 1979, Kalin 1 reported the unbelievable self-surgeries of a young man who successfully performed a bilateral orchiectomy and two months later attempted to denervate his adrenal glands. The second surgery was aborted because he became tired and was experiencing more pain than anticipated  retracting his liver. Therefore, he came to the hospital for wound closure and postoperative care.  There have been numerous cases of self-mutilation but this patient was unique because of his understanding and skill. Kalin was unable to locate any previous accounts of abdominal self-surgery. I recently discovered a case with startling similarities that occurred 100 years earlier. 2   Report of a Case.—  A 37-year-old man performed complete circumcision on himself. Then, in order to see what was inside, he slit up his abdomen from the symphysis to the navel. He came to a hospital with prolapsed peritoneum and intestines but was soon