tbuckley/cabot-search huggingface dataset:
Abstract 15675: Bone Marrow Derived Ixmyelocel-T From Patients With Severe Ischemic Dilated Cardiomyopathy Display Robust Anti-Inflammatory and Pro-Angiogenic Properties
Authors: Kelly J Ledford, Ann E Remmers, Ross Tubo
Journal: Circulation (2014)

Ixmyelocel-T, an expanded autologous multicellular therapy cultured from BMMNCs consisting of regenerative MSCs and M2-like macrophages, has shown clinical promise in the treatment of ischemic DCM. However the quality of BM-derived cell therapies from patients with severe heart failure is unclear as reports suggest they are dysfunctional due to advanced age and the systemic disease state. We hypothesized that ixmyelocel-T generated from ischemic DCM patients BMMNCs would consist of expanded MSCs and M2 macrophages, and produce anti-inflammatory and angiogenic factors useful for cardiac repair. BMMNCs were obtained from 55-80 year old ischemic DCM patients, with LVEF &lt; 35% and NYHA Class III or IV, enrolled in the phase 2b clinical IXCELL-DCM trial. The BMMNCs were either used in experiments or expanded in a closed bioreactor system for 12 days to generate ixmyelocel-T (n = 13). FACS revealed a 50 ± 5% fold expansion in CD90+ MSC and a 96 ± 39% fold expansion in CD14+ M2-like macrophages in ixmyelocel-T. Cytokine arrays revealed ixmyelocel-T secreted more regulatory and trophic factors compared to BMMNCs. Specifically, ixmyelocel-T secreted significantly more Flt-3L, GM-CSF, IL-10, IL-13, IL-1ra, IL-2, IL-3, IL-5, MCP-1, CCL18, TGF-β, angiopoietin-2, IL-8, TIMP-1, TIMP-2, and VEGF compared to BMMNCs. ELISA analysis confirmed that ixmyelocel-T secreted significantly higher levels of IL-10 (2092 ± 183 vs 130 ± 17 pg/mL, p &lt; 0.001) and IL-1ra (12241 ± 2806 vs 1857 ± 564, p &lt; 0.001) after LPS challenge. Ixmyelocel-T had higher gene expression of the anti-inflammatory and pro-angiogenic markers HMOX1, CCL18, FGF2, IGF1, PGF, SDF-1α, Ang-1, Ang-2 and lower levels of the pro-inflammatory markers TNFα, IL-1β, IFN[[Unable to Display Character: &amp;#611;]], IL-12 compared to BMMNCs after LPS challenge. Additionally, co-culture of ixmyelocel-T with HUVECs resulted in significant wound healing in an in vitro scratch assay compared to BMMNCs (56 ± 4 vs 39 ± 8%, p &lt; 0.001). This data suggests that the process used to generate ixmyelocel-T is ideal for the therapeutic expansion of BMMNCs from patients with severe cardiac diseases. Ixmyelocel-T retains a regenerative, anti-inflammatory, and pro-angiogenic profile which holds promise for treatment in advanced cardiac diseases where treatment options are limited.
STATISTICAL STUDY OF 6,000 CASES OF DIABETES
Authors: H J JOHN
Journal: Annals of Internal Medicine (1950)

Article1 October 1950STATISTICAL STUDY OF 6,000 CASES OF DIABETESHENRY J. JOHN, M.D., F.A.C.P.HENRY J. JOHN, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-33-4-925 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptINCIDENCEThe incidence of diabetes in the total population is usually given as approximately 1 per cent. In reality, we do not know exactly what the incidence is. Gradually, however, we are getting more information. Data presented in this paper are derived from the records of 6,000 patients seen in the period from 1921 to 1948.From 1921 to 1933, while I was at the Cleveland Clinic, I studied carefully the incidence of diabetes among all patients admitted to this institution (figure 1). During the last seven years of this period, the percentage of diabetics in this large group of...Bibliography1. WilkersonKrall HLLP: Diabetes in a New England town. A study of 3,516 persons in Oxford, Mass., J. A. M. A. 135: 209-216 (Sept. 27) 1947. CrossrefMedlineGoogle Scholar2. John HJ: Diabetes, a statistical study of 1,000 cases, Arch. Int. Med. 39: 67-92, 1927. A statistical study of 2,000 cases of diabetes, Arch. Int. Med. 42: 217-248, 1928. CrossrefGoogle Scholar3. Hansen S: Heredity of diabetes, Acta med. Scandinav. 62: 1-196, 1925. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: *Received for publication November 6, 1948. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byFettsucht und Diabetes mellitusLiteraturPeripheral Arterial Insufficiency: An Epidemiological Study of 2.243 Hospital Admissions Caused by Arteriosclerosis Obliterans, Diabetes Mellitus, Thrombangitis Obliterans and Arterial EmbolismDie Verbreitung des Diabetes mellitus: Häufigkeit und Vorkommen in Europa und AmerikaA CLINICAL STUDY OF THYROID STORM*†SHELDON S. WALDSTEIN, M.D., SHELDON J. SLODKI, M.D., G. IRENE KAGANIEC, M.D., DAVID BRONSKY, M.D.Thyrotoxic Crisis and Diabetic KetoacidosisWachstum, Erblichkeit und Manifestationsalter beim Diabetes mellitus von Kindern und JugendlichenThe Mortality in Diabetes Mellitus in Some European Countries 1935-1949Current Judgments on Metabolic Control and Complications in DiabetesA Twenty-Year Hospital Survey of Diabetic DeathsAbstracts on nutrition 1 October 1950Volume 33, Issue 4Page: 925-940 ePublished: 1 December 2008 Issue Published: 1 October 1950 PDF downloadLoading ...
Eotaxin induces degranulation and chemotaxis of eosinophils through the activation of ERK2 and p38 mitogen-activated protein kinases
Authors: Gita T. Kampen, Susan Stafford, Tetsuya Adachi, Tan Jinquan, Sha Quan, J. Andrew Grant, Per S. Skov, Lars K. Poulsen, Rafeul Alam
Journal: Blood (2000)
Abstract 19146: Increasing Donor Age is a Risk Factor for Permanent Pacing After Orthotopic Heart Transplantation
Authors: Ferdi Akca, Tamas Szili-Torok, Rahat Muslem, Olivier Manintveld, Alina Constantinescu, Ozcan Birim, Kadir Caliskan
Journal: Circulation (2014)

Introduction:  Due to extreme scarcity of donor hearts in The Netherlands, increasingly older and marginal donor hearts are accepted for orthotopic heart transplantation (HTx). We evaluated the relationship between the age of the donor and the need for pacemaker (PM) implantation early or late after HTx.   Methods:  The demographic and clinical data of all consecutive patients transplanted between 1984 until 2014 at our center were analyzed (n=612, 74% male; median follow up of 97 months [IQR 38-161]). Three groups were created based on donor age: Group I (0-35 y, n=345), Group II (35-50 y, n=190) and Group III (>50 y, n=77). The primary outcome was permanent PM implantation after HTx for either sinus node dysfunction (SND) or AV block (AVD). The need for early (90 days) was evaluated. The following secondary parameters were analyzed: recipient age, ischemic time, and death.   Results:  The mean recipient age at HTx was 46.4 ± 14.3 years [range 2-72 y] and donor age 32.8 ± 13.7 y. Significant older donor hearts were transplanted during the last ten years compared to the first ten years (40.1±15.9 y vs. 25.7±8.3 y, p   Conclusions:  Increasing donor age, particularly > 50 years, is a major risk factor for the need of permanent pacing post-HTx.
Abstract 19049: CASK Regulates Sodium Channels Distribution and Function in Costameric Microdomain
Authors: Catherine A Eichel, Lin Xianming, Florent Louault, Gilles Dilanian, Mario Delmar, Stéphane N Hatem, Elise Balse
Journal: Circulation (2014)

Introduction: Proteins of the MAGUK family have emerged as key components in ion channels organization and regulation within specialized submembrane domains of cardiomyocytes. In this context, we investigated for the first time the expression, localization and function in the heart of the MAGUK protein CASK. Methods and Results: Surprisingly, no signal was detectable for CASK at intercalated discs, making CASK the first MAGUK to be excluded from these structures. On the contrary, CASK was located at the lateral membrane where it belonged specifically to the costameric dystrophin/glycoproteins complex (DGC). Since a lateral sub-population of Nav1.5 channels have been reported to interact with syntrophin, a member of DGC, we hypothesized that CASK could modulate Nav1.5 channels. Using high resolution 3-D deconvolution microscopy, we observed a co-localization of CASK and Nav1.5 in cardiomyocytes and co-IP experiments confirmed that the two proteins are in the same complex. Whole-cell patch-clamp recordings revealed a negative regulation by CASK of the sodium current INa carried by Nav1.5 channels in cardiomyocytes. Using super resolution scanning coupled to conventional cell-attached patch-clamp, we investigated the involvement of CASK on Nav1.5 function and localization in highly confined microdomains of the lateral membrane. INa was recorded in crests and T-tubules on freshly isolated cardiomyocytes obtained from adult rats injected with Adeno Associated Virus (eGFP-AAV-ShCASK or eGFP-AAV-ShScr). CASK silencing caused a drastic reduction of INa at the crest whereas it increased the current at the T-tubules suggesting that CASK retains Nav1.5 channels at the crest and prevents their clustering in T-tubules. In vivo CASK silencing in rat using an eGFP-AAV-ShCASK-injected was associated with a conduction slowing as indicated by the prolongation of the QRS duration, cardiac dilation and left ventricle systolic dysfunction. Conclusion: Taken together these results indicate that CASK is a major determinant of the organization of a subpopulation of Nav1.5 at the costamere of cardiomyocytes and that CASK-silencing induces electrical and morphological remodeling.
Suboptimal care of patients before admission to intensive care
Authors: C. Garrard, D. Young
Journal: BMJ (1998)

Papers(p 1853 

During the past decade deficiencies in the quality of medical care have precipitated detailed scrutiny in the form of national confidential inquiries. These inquiries have examined perioperative deaths (NCEPOD), maternal deaths, and more recently, babies' deaths.1 2–3The 1993 NCEPOD report showed that two thirds of perioperative deaths occurred three or more days after surgery, usually from cardiorespiratory complications and in a ward environment. The riskiness of ward care is illustrated again this week in a different sort of confidential inquiry.

On p 0000 McQuillan and colleagues present the results of a confidential inquiry into the quality of care received by 100 patients admitted to intensive care (p 0000).4 After conducting structured interviews with the referring and intensive care clinical teams, the investigators completed a questionnaire that focused on the recognition, investigation, monitoring, and management of each patient's airway, breathing, and circulation (ABCs). Two independent assessors (a nephrologist and an anaesthetist) evaluated the resulting questionnaires. Both agreed that 54 of the 100 patients received suboptimal care. Mortality in the intensive care unit for these patients was 48%, almost twice that of the 20 patients who they agreed had been managed well. In addition, two thirds of these …
Abstract 11644: Infective Endocarditis Due to Staphylococcus Aureus and Lethality Are Critically Dependent on the Action of Superantigens
Authors: Christopher S Stach, Bao G Vu, Joseph A Merriman, Alfa Herrera, Patrick M Schlievert, Wilmara Salgado-Pabón
Journal: Circulation (2014)

Introduction:  Staphylococcus aureus  infective endocarditis (IE) is an aggressive infection of the heart, predominantly the valves, that is fulminant and becomes life threatening in a matter of days. It affects ~40,000 individuals each year in the US, resulting in ~12,000 strokes and 20,000 deaths. In  S. aureus  illnesses, superantigens (SAgs) cause lethality through massive immune system activation and their effects on the cardiovasculature, eliciting capillary leak and hypotension. Up to 90% of  S. aureus  IE isolates encode for the enterotoxin gene cluster ( egc ) SAgs, 9-20% for toxic shock syndrome toxin-1 (TSST-1), and 18-25% for staphylococcal enterotoxin C (SEC). SEC is critical for vegetation formation and death in  S. aureus  MW2, a human isolate exceptional at causing IE in rabbits. Here, we addressed the contribution of  egc  SAgs and TSST-1 to expand on the role of SAgs to IE disease severity and outcomes.   Methods:  In this study, we tested  S. aureus  strain MN8, a human isolate that causes IE in rabbits and produces TSST-1 and  egc  SAgs, and SAg deletion isogenic variants in the rabbit model of native valve, left-sided IE.   Results:  Deletion of  tstH  and the  egc  significantly reduced vegetation size and increased survival. However, TSST-1 and the  egc  SAgs have differential effects on survival.  tstH  deletion leads to rabbit survival similar to that of infection with  tstHegc , while the  egc  deletion leads to lethality resembling that of wildtype. While  tstH  deletion increases survival, it still results in large septic vegetations and late lethality in 50% of rabbits. Furthermore, expression of TSST-1 or  egc  SAgs in SAg negative strains results in large septic vegetations, demonstrating that production of SAgs is sufficient to allow an otherwise avirulent strain to cause IE.   Conclusion:  The  egc  SAgs, TSST-1 and SEC are critical contributors to the etiology and fatal outcomes characteristic of  S. aureus  IE. A decrease in morbidity and mortality due to  S. aureus  IE may be feasible with standard of care that incorporates SAg neutralizing agents or SAg production inhibitors to allow antimicrobial therapy to take on its full effect.
Re: Jimsgene Sanjmyatav, Sven Hauke, Mieczyslaw Gajda, et al. Establishment of a Multicolour Fluorescence In Situ Hybridisation-based Assay for Subtyping of Renal Cell Tumours. Eur Urol 2013;64:689–91
Authors: Jérôme Verine, Christophe Leboeuf, Philippe Ratajczak
Journal: European Urology (2013)
Shared Medical Decision Making
Authors: Maria J. Silveira, Chris Feudtner
Journal: JAMA (2005)

To the Editor: I am concerned with the conclusion of the Commentary by Dr McNutt on shared medical decision making. I cannot understand why any physician would want to “maximize decisional conflict” in a patient already stressed with an illness and decrease the chance of a positive patient outcome. It appears that the described process of decision making is more for the benefit of the physician than the patient. My patients express 2 common desires: to be treated with dignity and to have a caring relationship with their physician. The former includes honestly educating patients about prescribed treatments, including possible negative outcomes. However, the latter sometimes demands that physicians extend themselves as they attempt to educate patients, even when they know that words shape concepts. Doing so without prejudice can be difficult, but not doing so is to abdicate an important responsibility. Simply tossing out numbers and sitting back with arms folded while patients sweat over information they may not understand may leave the physician less stressed, but it is not good medicine. There is a middle ground between arm folding and dictating treatment in a paternalistic fashion. Decisions characterized as “not really to be made by the patient” and those where patients are supposed to reflect are not members of 2 different sets—they fall on a continuum. Consider religious sects that oppose the use of antibiotics or the transfusion of blood. Decision making in these 2 circumstances cannot ignore patient input even though clinical realities might suggest only 1 rational treatment. Finally, unilaterally declaring that patients will learn to accept the responsibility of choosing is arbitrary at best. Patients typically experience serious illness as a pop-quiz for which they have not prepared. They bring to the test personal strengths and weaknesses and lifelong ethical viewpoints. Appropriate care should include working to understand these rather than forcing any change. I also found the “navigator/pilot” metaphor misleading. If a pilot crashes, the navigator usually crashes along with him—the navigator shares the same risk as a pilot. I believe that is the opposite of what the metaphor was intended to convey. The vagaries of life preclude absolute assurances about anything. Perhaps our most appropriate goal is to help patients find comfort with whatever decision they make.
Micro-CT and 18F-FDG micro-PET of pulmonary fibrosis in mice induced by adenoviral gene transfer of TGF-β1
Authors: Thomas Rodt, Christian von Falck, Roman Halter, Claudia Boehm, Matthias Luepke, Florian Laenger, Jack Gauldie, Juergen Borlak, Frank Wacker, Ulrich Maus
Journal: European Respiratory Journal (2011)

Introduction:  The morphological and functional information provided by micro-CT and micro-PET allows monitoring of acute and chronic disease states in small laboratory animals. We examined in-vivo micro-CT and micro-PET as non-invasive tools to assess pulmonary fibrosis in mice.   Material/Methods:  Pulmonary fibrosis was induced in mice by intratracheal delivery of an adenoviral gene vector encoding biologically active TGF-β1. Respiratory gated and ungated micro-CT was performed in 18 mice at 1 to 4 weeks after pulmonary adenoviral gene vector delivery. In 5 additional mice  18 F-FDG micro-PET and micro-CT was performed. Imaging was correlated to histopathology and findings in animals exposed to a control vector. Radiation doses were measured using thermoluminescence dosimeters.   Results:  Significant correlation between Ashcroft histology scoring and micro-CT was found for visual assessment scoring (p 18 F-FDG micro-PET showed slight increase of glucose metabolism in the consolidated lung areas determined by micro-CT, which was coregistered to the micro-PET data using anatomical landmarks. Radiation doses for micro-CT ranged from 174 to 277 mSv. For micro-PET an expected dose of 140 mSv was calculated from the measurements.   Conclusion:  Micro-CT and micro-PET allow valid visualisation of morphology and metabolism for the assessment of fibrosis in mice. The measured radiation doses allow serial examinations without deterministic radiation effects.
The Church Supper
Authors: Jeanette M. Smith
Journal: JAMA (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Suicidal feelings in a population sample of nondemented 85-year-olds
Authors: I Skoog, O Aevarsson, J Beskow, L Larsson, S Palsson, M Waern, S Landahl, S Ostling
Journal: American Journal of Psychiatry (1996)

The authors studied the 1-month frequency of suicidal feelings among very old people.A population sample (N = 345) of nondemented 85-year-olds in Gothenburg, Sweden, were examined by a psychiatrist. Suicidal feelings were rated by the system of Paykel et al. Mental disorders were diagnosed according to DSM-III-R.Of the mentally healthy subjects (N = 225), 4.0% had thought during the last month that life was not worth living, 4.0% had had death wishes, and 0.9% had thought of taking their own lives. None had seriously considered suicide. The figures were higher among subjects with mental disorders (N = 120); 29.2% had thought that life was not worth living, 27.5% had had death wishes, 9.2% had thought about taking their lives, and 1.7% had seriously considered suicide. Among the subjects with mental disorders, including depression, suicidal feelings were associated with greater use of anxiolytics but not of antidepressants. Women who felt that life was not worth living had a higher 3-year mortality rate than did women without these feelings (43.2% versus 14.2%). This finding was independent of concomitant physical and mental disorders.Mild suicidal feelings are common in elderly subjects with metal disorders but infrequent in the mentally healthy. The substantially higher mortality rate in women who felt that life was not worth living, compared to women who did not, suggests these feelings must be taken seriously. Because of the high suicide rate in the elderly, there is a need for better diagnosis and treatment of mental disorders in this age group.
Abstract 15548: Screening Entire Health System ECG Databases to Identify Patients with Arrhythmogenic Myocardial Substrate at Increased Risk of Death
Authors: David G Strauss, Nathan Mewton, Richard L Verrier, Bruce Nearing, Tony Killian, Francis E Marchlinsky, Larisa G Tereshchenko, Katherine C Wu, Christopher Cox, Peter M Spooner, Joao A Lima
Journal: Circulation (2011)

David G Strauss, 2011 Finalist and Presenting Author
Pattern recognition receptors TLR4 and CD14 mediate response to respiratory syncytial virus
Authors: Evelyn A. Kurt-Jones, Lana Popova, Laura Kwinn, Lia M. Haynes, Les P. Jones, Ralph A. Tripp, Edward E. Walsh, Mason W. Freeman, Douglas T. Golenbock, Larry J. Anderson, Robert W. Finberg
Journal: Nature Immunology (2000)
The prevalence of age-related maculopathy by geographic region and ethnicity1This paper has been edited by Neville N. Osborne, PhD, DSc, Nuffield Laboratory of Ophthalmology, University of Oxford, Walton Street, Oxford, UK; and Gerald J. Chader, The Foundation Fighting Blindness, Hunt Valley, MS.1
Authors: Ronald Klein, Barbara E.K Klein, Karen J Cruickshanks
Journal: Progress in Retinal and Eye Research (1999)
Increased density of ecto 5' nucleotidase antigen on leukemic T cells from patients with cutaneous T-cell lymphoma and adult T-cell leukemia/lymphoma
Authors: Y Fukunaga, SS Evans, M Yamamoto, Y Ueda, K Tamura, T Takakuwa, D Gebhard, J Allopenna, S Demaria, B Clarkson
Journal: Blood (1989)
Risk factors and HDL.
Authors: A Rotsztain
Journal: Circulation (1978)

described in our paper.Most of our patients were not symptomatic but were evaluated because of detection of a cardiac murmur.Hence, they probably represent an earlier phase of the disorder.In the patient who underwent catheterization, left ventriculography did not show evidence of cavity obliteration, but this study was per- formed at a time when the patient's murmur could not be heard.No attempt was made to provoke an intracavitary pressure gradient during catheterization.