tbuckley/cabot-search huggingface dataset:
Breaking down language barriers
Authors: D. Jones, P. Gill
Journal: BMJ (1998)

<h3>Background</h3> Chimeric antigen receptor (CAR) T cells engineered to recognize and target tumor associated antigens have made a profound impact on the quality of life for many patients with cancer. However, tumor heterogeneity and intratumoral immune suppression reduce the efficacy of this approach, allowing for tumor cells devoid of the target antigen to seed disease recurrence. Here, we address the complexity of tumor heterogeneity by developing a universal CAR. <h3>Method</h3> We constructed a universal Fabrack-CAR with an extracellular domain composed of the non-tumor targeted, cyclic, twelve residue meditope peptide that binds specifically to an engineered binding pocket within the Fab arm of monoclonal antibodies (mAbs). As this site is readily grafted onto therapeutic mAbs, the antigen specificity of these universal Fabrack-CAR T cells is simply conferred by administering mAbs with specificity to the heterogeneous tumor. <h3>Results</h3> Using in vitro and in vivo studies with multiple meditope-engineered mAbs, we show the feasibility, specificity, and robustness of this approach. These studies demonstrate antigen- and antibody-specific T cell activation, proliferation, and IFNγ production, selective killing of target cells in a mixed population, and tumor regression in animal models. <h3>Conclusion</h3> Collectively, these findings support the feasibility of this universal Fabrack-CAR T cell approach and provide the rationale for future clinical use in cancer immunotherapy.
Primary cutaneous nocardiosis caused by Nocardia beijingensis
Authors: C. Derancourt, R. Theodose, L. Deschamps, B. Liautaud, E. Briand, S. Derancourt, N. Desbois, A. Le Fleche Mateos
Journal: British Journal of Dermatology (2012)

Journal Article Primary cutaneous nocardiosis caused by Nocardia beijingensis Get access C. Derancourt, C. Derancourt Departments of Dermatology Search for other works by this author on: Oxford Academic Google Scholar R. Theodose, R. Theodose Microbiology Search for other works by this author on: Oxford Academic Google Scholar L. Deschamps, L. Deschamps Pathology Search for other works by this author on: Oxford Academic Google Scholar B. Liautaud, B. Liautaud Infectiology, University Hospital, Fort de France 97261, Martinique, France Search for other works by this author on: Oxford Academic Google Scholar E. Briand, E. Briand Department of Plastic Surgery, Saint Paul Clinic, Fort de France, Martinique, France Search for other works by this author on: Oxford Academic Google Scholar S. Derancourt, S. Derancourt Dermatology Private Practice, Rivière Salée, Martinique, France Search for other works by this author on: Oxford Academic Google Scholar N. Desbois, N. Desbois Departments of Dermatology Search for other works by this author on: Oxford Academic Google Scholar A. Le Fleche Mateos A. Le Fleche Mateos Pasteur Institute, Bacterial Identification Centre, Paris, France E‐mail: christian.derancourt@chu‐fortdefrance.fr Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 167, Issue 1, 1 July 2012, Pages 216–218, https://doi.org/10.1111/j.1365-2133.2012.10832.x Published: 01 July 2012
Abstract 20669: Urocortin-2 Improves Right Ventricular Function in Pulmonary Arterial Hypertension
Authors: Diana Santos-Ribeiro, Rui Adão, Carolina Maia-Rocha, Bárbara Alves, Pedro Mendes-Ferreira, Adelino F Leite-Moreira, Carmen Brás-Silva
Journal: Circulation (2014)

Introduction: Urocortin (UCN)-2 is a peptide highly expressed in the cardiovascular system and it has shown promising therapeutic effects in several studies in both humans and animal models of heart failure (HF). However, the role of UCN-2 in right ventricular (RV) failure is still unknown. Hypothesis: This study analyzed the effects of UCN-2 treatment in an animal model of RV HF, secondary to pulmonary arterial hypertension (PAH). Methods: Male Wistar rats (180-200g) randomly received monocrotaline (MCT, 60mg/Kg, s.c.) or vehicle. After 14 days, animals from these groups were randomly assigned to receive treatment with either UCN-2 (2.5μg/Kg/day, i.p.) or vehicle. The study resulted in 4 groups: control (CTRL) (n=12); CTRL+UCN-2 (n=10); MCT (n=9); MCT+UCN-2 (n=8). RV Pressure-Volume measurements were performed 24-25 days after MCT administration. Only significant results (mean±SEM, p&lt;0.05) are given. Results: MCT group developed PAH, as shown by: increased RV end-systolic pressure (MCT vs CTRL: 59±3 vs 26±1 mmHg) and end-diastolic pressure (MCT vs CTRL: 6.7±0.9 vs 3.5±0.7 mmHg), RV dilatation (MCT vs CTRL: 270±17 vs 215±12 μL), and decreased cardiac output (MCT vs CTRL: 29±3 vs 63±3 mL/min) and ejection fraction (MCT vs CTRL: 37±6 vs 72±2%). UCN-2 treatment resulted in attenuation of RV pressure increase (RVESP: 42±2 mmHg; RVEDP: 4.7±0.6 mmHg), dilatation (RVEDV: 212±4 μL), and in improved cardiac function (CO: 48±2 mL/min; EF: 58±2%). Conclusions: UCN-2 chronic treatment significantly reduced the worsening of RV function in PAH. These findings suggest that the UCN-2 pathway has a relevant role on the pathophysiology of PAH and RV failure, representing a potential therapeutic target.
Anti-CD20 monoclonal antibody (rituximab) in the treatment of pemphigus
Authors: M.J. Arin, A. Engert, T. Krieg, N. Hunzelmann
Journal: British Journal of Dermatology (2005)

Journal Article Anti‐CD20 monoclonal antibody (rituximab) in the treatment of pemphigus Get access M.J. Arin, M.J. Arin Departments of Dermatology and Internal Medicine I, University of Cologne, 50924 Cologne, Germany Search for other works by this author on: Oxford Academic Google Scholar A. Engert, A. Engert Departments of Dermatology and Internal Medicine I, University of Cologne, 50924 Cologne, Germany Search for other works by this author on: Oxford Academic Google Scholar T. Krieg, T. Krieg Departments of Dermatology and Internal Medicine I, University of Cologne, 50924 Cologne, Germany Search for other works by this author on: Oxford Academic Google Scholar N. Hunzelmann N. Hunzelmann Departments of Dermatology and Internal Medicine I, University of Cologne, 50924 Cologne, Germany Nicolas Hunzelmann. E‐mail: nico.hunzelmann@uni‐koeln.de Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 153, Issue 3, 1 September 2005, Pages 620–625, https://doi.org/10.1111/j.1365-2133.2005.06651.x Published: 01 September 2005 Article history Accepted: 09 January 2005 Published: 01 September 2005
Caspase-independent mitotic apoptosis (CIMA): a novel type of caspase-independent cell death that occurs during mitosis
Authors: Yohei Niikura, Katsumi Kitagawa
Journal: Cancer Research (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

4906 

The spindle checkpoint protects cells from aneuploidy by monitoring the status of the kinetochore-microtubule attachment. When cells have defects in this mitotic checkpoint pathway and in kinetochore-microtubule attachment, substantial aneuploidy is thought to result. We report here a novel type of caspase-independent apoptosis that occurs during mitosis_CIMA (caspase-independent mitotic apoptosis). Simultaneous depletion of BUB1, but not MAD2, and treatment with nocodazole (a microtubule-depolymerizing drug), paclitaxel (Taxol, a microtubule-stabilizing drug), or 17-AAG (17-allylaminogeldanamycin, an HSP90 inhibitor) induced DNA fragmentation in cells during early mitosis. Because the common defects caused by the microtubule inhibitors and 17-AAG are those in kinetochore-microtubule attachment, we conclude that CIMA occurs when the kinetochore-microtubule attachment is altered and BUB1 function is altered. This novel mitotic cell death appeared to be independent of caspase activation. We found that AIF (apoptosis-inducing factor) and EndoG (endonuclease G), which are effectors of caspase-independent apoptosis, are released from mitochondria during the activation of CIMA, and that DNA fragmentation is dependent on EndoG. Our results suggest that BUB1 is a critical factor in regulating CIMA. We propose that CIMA protects cells from aneuploidy by inducing the death of cells prone to substantial chromosome missegregation.
Abstract 15412: Non-viral Direct Reprogramming of Fibroblasts Into a Three Dimensional Vascularized Cardiomimetic Tissue
Authors: Jaeyeaon Cho, Sangsung Kim, Hun-Jun Park, Ming-ke Song, Sang Wook Lee, Jae kyung Jung, Shang Ping Yu, Mark A Sussman, Richard P Harvey, Young-sup Yoon
Journal: Circulation (2014)

Background and Hypothesis:  Transcription factors or microRNAs were demonstrated to directly reprogram fibroblasts into cardiomyocytes (CMs), endothelial cells (ECs), or smooth muscle cells (SMCs). However, simultaneous reprogramming into CMs and vascular cells has not been reported. Several muscle-specific miRNAs are involved in cardiac development and differentiation. Accordingly, we hypothesized that these muscle-specific miRNAs could reprogram fibroblasts into various cardiac cell types.   Methods and Results:  We transfected miRNA mimics miR-1a-2-5p, miR-208a-3p, miR-208b-3p, miR-208b-5p or miR-499-5p separately or in combinations, into adult mouse tail-tip fibroblasts w/wo ascorbic acid (AA) and BMP4. qRT-PCR analyses showed that miR-208b-3p most highly induced mRNA expression of CM-, EC-, SMC-marker genes. A combined treatment of AA and BMP4 enhanced reprogramming efficiency and maturation, as evidenced by spontaneous contractions of reprogrammed CM (rCMs) and formation of vascular network by reprogrammed ECs (rECs) and reprogrammed SMCs (rSMCs). The rECs accounted for 37 ± 6 % of the total cells. Ten days after the treatment, a substantial amount of extracellular matrix was deposited, leading to formation of a tissue-like patch referred to as cardiomimetic tissue (CMT). We transplanted CMT labeled with CM-Dil or generated from fibroblasts of GFP transgenic mice onto the apex of the mouse heart. At week 4, histologic examination of the heart sections showed robust engraftment and retention of CMT-derived cells. Systemic perfusion of BLS-1 lectin at week 4 revealed formation of functional blood vessels by rECs alone or together with host ECs. In addition, rCMs formed mass-like structures, consisting of 11.9 ± 4.4 % cells in the transplanted CMT, and showed clear striations and GJA1 (Connexin 43) expression between cardiomyocytes.  In conclusion, we for the first time demonstrated that a combined treatment of a specific miRNA mimic, AA, and BMP4 is able to reprogram postnatal fibroblasts into functional CM-, EC- and SMCs-like cells, induce formation of ECMs, and generate a cardiac-tissue like structure. This novel tissue reprogramming can serve as a platform for cell therapy, disease modeling and drug development.
Phenotypic concordance and time clustering of diagnosis in multiply affected ibd families : the IOIBD Multiplex Family Collaborative Project, phases I a II
Authors: C. Tamboli, A. Attaluri, J. Yan, A. Duke, J. F. Colombel, M. Silverberg, R. McLeod, D. Jewell, S. Goldthorpe, G. Vernier, S. Vermeire, M. Joossens, J. O. Chapman, C. Bernstein, V. Annese, G. Lombardi, M. B. Bengtson, M. Vatn, Jonas Halfvarson, C. Tysk, T. Jess, L. Riis, A. Timmer, A. Bauer, B. Korelitz, K. Sultan, W. Reinisch, L. Biancone, F. Pallone, T. Hibi
Journal: Gut (2005)

Phenotypic concordance and time clustering of diagnosis in multiply affected ibd families : the IOIBD Multiplex Family Collaborative Project, phases I & II
Novel approaches to the diagnosis of Strongyloides stercoralis infection
Authors: D. Buonfrate, F. Formenti, F. Perandin, Z. Bisoffi
Journal: Clinical Microbiology and Infection (2015)

Strongyloides stercoralis differs from the other soil-transmitted helminths because it puts infected subjects at risk of a fatal syndrome (in cases of immunosuppression for medical conditions, immunosuppressant therapies, or both). Chronic strongyloidiasis is often a non-severe condition, or is sometimes even asymptomatic, but diagnosis and effective therapy are essential in order to eradicate the infection and the life-long risk involved. Therefore, diagnostic methods need to be highly sensitive. Stool microscopy and the Kato-Katz technique are commonly used in prevalence studies, but they are inadequate for S. stercoralis detection. This is probably the main reason why the global prevalence has long been underestimated. Concentration methods, the Baermann technique and Koga agar plate culture have better, but still unsatisfactory, sensitivity. Serological tests have demonstrated higher sensitivity; although some authors have concerns about their specificity, it is possible to define cut-off values over which infection is almost certain. In particular, the luciferase immunoprecipitation system technique combined with a recombinant antigen (NIE) demonstrated a specificity of almost 100%. ELISA coproantigen detection has also shown promising results, but still needs full evaluation. Molecular diagnostic methods are currently available in a few referral centres as in-house techniques. In this review, on the basis of the performance of the different diagnostic methods, we outline diagnostic strategies that could be proposed for different purposes, such as: prevalence studies in endemic areas; individual diagnosis and screening; and monitoring of cure in clinical care and clinical trials.
Airway-related complications in preterm infants who were intubated at birth
Authors: Pedro Mondejar-Lopez, Mª Dolores Pastor-Vivero, Esther Bragado-Alcaraz, Juan José Quesada-Lopez, Virginia Perez-Fernandez, Manuel Sanchez-Solis, Luis Garcia-Marcos
Journal: European Respiratory Journal (2011)

Background: Airway (AW) complications associated with intubation (IT) and mechanical ventilation (MV) have been observed in preterm infants (PTI).

Methods: Fiberbronchoscopy (FB) was performed in symptomatic PTI who were intubated at birth. We describe their clinical manifestations (CM) and FB findings. Chi2 test was used to assess the association between left vocal cord paralysis (LVCP) and patent ductus arteriosus ligation (PDAL). Two logistic regressions were built using “condition likely to be associated with IT and MV (CAIT&MV*)” and “need for tracheostomy” as dependent variables. Gender, gestational age (GA), birth weight (BW), length of IT and PDAL were independent ones.

Results: 42 symptomatic PTI underwent a FB: 55% males; age 10±14 mo; GA 27±3 wk; BW 1070±467 gr; IT duration 31±28 d. The most common CM were stridor (76%), dysphonia (38%), failed extubation (12%) and suspicion of upper AW obstruction in lung function tests (12%). AW abnormalities were identified in all PTI: AW malacia (59%), laryngotracheal cyst or granuloma* (45%), LVCP (33%), subglottic stenosis* (30%), supraglottic oedema/hypertrophia* (21%) and tracheal stenosis* (7%). 27 PTI underwent PDAL, 9 tracheostomy and 6 AW surgery. All PTI with LVCP had undergone PDAL (p<0.0001).

View this table:

Logistic regression





Conclusions: All symptomatic PTI had abnormal FB findings. LVCP is strongly associated with PDAL. Each day of invasive MV increases by 9% the risk of acquired AW anomalies and by 6% the need for tracheostomy.
Abstract 1703: Nicotine-induced gemcitabine resistance is reversed by gamma-aminobutyric acid but enhanced by baclofen in pancreatic cancer xenografts and in pancreatic cancer cells <i>in vitro</i>
Authors: Jheelam Banerjee, Hussein A. N Al-Wadei, Mohammed H. Al-Wadei, Koami Dagnon, Hildegard M. Schuller
Journal: Cancer Research (2014)

Abstract Pancreatic cancer is frequently resistant to cancer therapeutics. Smoking and alcoholism are risk factors and pancreatic cancer patients often undergo nicotine replacement therapy (NRT) and treatment for alcohol dependence. Based on our report that low dose nicotine within the range of NRT causes gemcitabine resistance in pancreatic cancer, our current study has tested the hypothesis that GABA or the selective GABA-B-R agonist baclofen used to treat alcohol dependence reverse nicotine-induced gemcitabine resistance in pancreatic cancer. Using pancreatic cancer cell lines BXPC-3 and PANC-1, our data show that GABA significantly reversed gemcitabine resistance induced by low dose nicotine in xenografts whereas baclofen did not. This effect of GABA was accompanied by decreases in cAMP, p-CREB, p-AKT, p-Src, p-ERK metalloproteinases-9 and -2 and EGR-1 and increases in cleaved caspase-3 in xenografts whereas baclofen had the opposite effects. In vitro exposure of cells to single doses or seven days of nicotine induced the protein expression of MMP-2, MMP-9 and EGR-1 and these responses were blocked by GABA. Baclofen downregulated the protein expression of GABA-B-Rs in xenograft tissues and in cells exposed to baclofen for seven days in vitro. This response was accompanied by inversed baclofen effects from inhibition of cAMP formation after single dose exposures to stimulation of cAMP formation in cells pretreated for seven days. These findings suggest GABA as a promising agent to overcome nicotine-induced gemcitabine resistance in pancreatic cancer whereas treatment of alcoholism by baclofen may increase gemcitabine resistance. Supported by grants RO1CA130888 and RO1CA042829 with the National Cancer Institute. Citation Format: Jheelam Banerjee, Hussein A. N Al-Wadei, Mohammed H. Al-Wadei, Koami Dagnon, Hildegard M. Schuller. Nicotine-induced gemcitabine resistance is reversed by gamma-aminobutyric acid but enhanced by baclofen in pancreatic cancer xenografts and in pancreatic cancer cells in vitro. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1703. doi:10.1158/1538-7445.AM2014-1703
Ürtikeryal lezyonlar: Eğer ürtiker değilse başka ne olabilir? Ürtikerin ayırıcı tanısı Kısım II. Sistemik hastalıklar
Authors: Anna Peroni, Chiara Colato, Donatella Schena, veGiampiero Girolomoni
Journal: Journal of the American Academy of Dermatology (2010)
Stakeholder health insurance: empowering the poorest patients Commentary: Stakeholder health insurance has disingenuous aims
Authors: D. G Green
Journal: BMJ (2001)

# Stakeholder health insurance: empowering the poorest patients {#article-title-2} The NHS aims to be universal, comprehensive, equitable, and of a high standard but fails to achieve its objectives. Are there any overseas systems that guarantee all their people—especially the poorest members of society—a higher standard of care than the NHS? An honest observer looking at countries such as Germany, France, or the Netherlands would have to answer this question with a resounding “yes.” Can we learn from them? #### Summary points The NHS does not meet its own objectives to be comprehensive, equitable, and of a high standard It has three long standing problems: underfunding, lack of competition, and lack of respect for individual choice Stakeholder health insurance would use the market to improve care for everyone Patients who opt out of the NHS would receive a tax rebate in proportion to their earnings One advantage of European social insurance is that the standard and range of cover is linked to judgments about affordability made by people who are self sufficient through work. In Germany and France, people can see on their pay slips how much they are paying for health care and form a view about whether the cost is justified. The state guarantee reflects personal preferences to a greater extent than a system financed from taxes. Put another way, the French and Germans have found a way of making the market serve everyone. The government's national plan for the NHS dismissed overseas alternatives because they failed the test of equity. But in which European countries would you find the widest gap between the standard of care enjoyed by the poorest people and the standard enjoyed by the rich? The people with most to lose are those who depend exclusively on the government for services. The wealthy can always take care of themselves. In countries such as Germany, France, and the … Correspondence to J Dixon
THE PSORIATIC CAPILLARY.
Authors: J. B. ROSS
Journal: British Journal of Dermatology (1964)

Journal Article THE PSORIATIC CAPILLARY: Its Nature and Vaule in the Identification of the Unaffected Psoriatic. Get access J. B. ROSS J. B. ROSS Late Registrar, Department of Dermatology, Royal Victoria Infirmary, University of Newcastle upon Tyne Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 76, Issue 12, 1 December 1964, Pages 511–518, https://doi.org/10.1111/j.1365-2133.1964.tb15500.x Published: 01 December 1964
152. Nucleofection and Phage phiC31 Integrase Mediate Stable Introduction of a Dystrophin Fusion Gene into Muscle Derived Stem Cell and Human Myoblasts
Authors: Torunn Elisabeth Tjelle, Rune Kjeken, Ragnar Salte, Iacob Mathiesen
Journal: Molecular Therapy (2004)

Intramuscular injection of plasmid DNA followed by electrical stimulation (electroporation) is an efficient mean for non viral delivery of DNA in vivo in smaller animals like mice and rats. In animals with increased body mass however, transfection is less efficient, often caused by the difficulty of DNA distribution relative to the electric field. We have developed a new device for the combined DNA injection and electroporation in which the injection needles also serve as electrodes. DNA is injected through two needles during insertion into the muscle and is thus distributed along the entire needle path enabling a perfect match between the DNA and the electric field. Using this device we have aimed to optimize electroporation conditions such as injection volume, DNA concentration, and field strength for use in larger animals.
Verteporfin photodynamic therapy retreatment of normal retina and choroid in the cynomolgus monkey11The Massachusetts Eye and Ear Infirmary is an owner of a patent covering the use of verteporfin and photodynamic therapy. Should the Massachusetts Eye and Ear Infirmary receive royalties or other financial remuneration related to that patent, Drs. Miller and Gragoudas would receive a share of same in accordance with the Massachusetts Eye and Ear Infirmary’s institutional Patent Policy and …
Authors: Martin H Reinke, Christina Canakis, Deeba Husain, Norman Michaud, Thomas J Flotte, Evangelos S Gragoudas, Joan W Miller
Journal: Ophthalmology (1999)
The Eye Operation
Authors: Daniel M. Albert
Journal: JAMA (2001)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal