tbuckley/cabot-search huggingface dataset:
Abstract 792: Usefulness of alternate-day administration of S-1 and leucovorin in a xenograft mouse model of colorectal cancer: A shorter drug-free interval leads to more efficient antitumor effects
Authors: Toshihiro Komura, Shinobu Ohnuma, Koh Miura, Tetsuhiko Shirasaka, Taiki Kajiwara, Katsuyoshi Kudoh, Sho Haneda, Masahide Toshima, Atsushi Kohyama, Hiroaki Musha, Takeshi Naitoh, Yu Katayose, Michiaki Unno
Journal: Cancer Research (2014)

Abstract [Background] A clinical trial of S-1 with leucovorin (S-1/LV) in metastatic colorectal cancer (CRC) patients demonstrated promising efficacy; however, the gastrointestinal toxicities were so severe that it has not been applied in the clinical setting. On the other hand, alternate-day administration of S-1 has been proposed to attenuate the adverse events without reducing its anticancer activity. The aim of this study was to confirm the feasibility of alternate-day administration of S-1/LV in in vivo xenograft tumor models. [Methods] Mice were treated with S-1/LV either in a daily group (two weeks of administration followed by two weeks of withdrawal) or an alternate-day group (administration on alternate days for four weeks), then the mice of both groups were sacrificed and the xenograft tumors were resected. To assess the adverse reactions, the body weight changes, the condition of feces, mucosal injury of small intestine and myelosuppression were compared between both groups. Furthermore, tumor volume, tumor growth inhibition (TGI) and the expression of Ki67, TUNEL, cIAP2 and XIAP were analyzed to evaluate the antitumor activity and tumor apoptosis. [Results] Severe weight loss, diarrhea, mucosal injury and myelosuppression were observed only in the daily group; however, no adverse reactions were observed in the alternate-day group, except slight myelosuppression. The TGI in the alternate-day group was better than that in the daily group, possibly resulting from apoptosis of tumor cells due to the suppression of cIAP2. [Conclusion] Our findings suggest that alternate-day administration of S-1/LV for CRC treatment can achieve higher antitumor activity without severe adverse reactions. Therefore, we propose that clinical trials with this regimen should be conducted in CRC patients. Citation Format: Toshihiro Komura, Shinobu Ohnuma, Koh Miura, Tetsuhiko Shirasaka, Taiki Kajiwara, Katsuyoshi Kudoh, Sho Haneda, Masahide Toshima, Atsushi Kohyama, Hiroaki Musha, Takeshi Naitoh, Yu Katayose, Michiaki Unno. Usefulness of alternate-day administration of S-1 and leucovorin in a xenograft mouse model of colorectal cancer: A shorter drug-free interval leads to more efficient antitumor effects. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 792. doi:10.1158/1538-7445.AM2014-792
The impact of multidrug resistance in healthcare-associated and nosocomial Gram-negative bacteraemia on mortality and length of stay: cohort study
Authors: D.C. Lye, A. Earnest, M.L. Ling, T.-E. Lee, H.-C. Yong, D.A. Fisher, P. Krishnan, L.-Y. Hsu
Journal: Clinical Microbiology and Infection (2011)
635. Macrophage Colony Stimulating Factor Expression in Retrovirally Transfected Cells Is Dependent upon the Adherence Status of the Target Cells
Authors: JianGang Zhang, Qinghong Dan, Timothy C. Fong, Christopher C. Williams, Maria D. Avina, Mehrdokht Tarbiyat-Boldaji, Sakineh Khalaghizadeh, Michael Irwin, Amy Nyugen, H. Terry Wepsic, Martin R. Jadus
Journal: Molecular Therapy (2004)

Numerous cell types including both normal and cancerous cells retrovirally transduced with either the membrane or the secreted forms of macrophage colony stimulating factor (mM-CSF and sM-CSF, respectively) using LXSN-based vectors showed a variable expression of the transgene. Expression of either form of M-CSF correlated with the cells' adhesion to culture dishes. Transduced adherent cells produced more sM-CSF by ELISA and mM-CSF by flow cytometry; whereas, the non-adherent cells synthesized little M-CSF. Use of a granulocyte-macrophage colony stimulating factor (GM-CSF) LXSN-based retrovirus failed to show this dichotomy. Real-time polymerase chain reaction analysis using the ΔΔCT method showed that adherent cells produced more M-CSF-specific mRNA than the non-adherent cells, while maintaining similar mRNA levels for the β-actin and neomycin resistance genes. Examination of the M-CSF genes used for creating these M-CSF retroviral vectors indicated that 170 base pairs of the 5′ flanking untranslated region of the M-CSF gene were present in the M-CSF retroviral constructs. Ligation of this 5′ region of the M-CSF gene to the 5′ proximal end of the enhanced green fluorescent protein gene (EGFP) caused the expression of EGFP to show the same dichotomy as previously seen with the M-CSF. This work suggests that this 5′ flanking region of the M-CSF gene could be an important way to get transgenic expression within adherent cells, but not in non-adherent cells, when differential gene expression is needed.
Abstract 5580: TGF-β1 enhanced cell migration involving pluripotent transcription factor OCT4 in endometriosis
Authors: Te-Sheng Chang, Heng-Kien Au, Thai-Yen Ling, Ching-Chi Chi, Chii-Ruey Tzeng, Jui-Hung Chang, Yen-Hua Huang
Journal: Cancer Research (2014)

Abstract Endometriosis is the eutopic and/or ectopic growth of endometrial tissues. High levels of TGF-β in peritoneal fluid and increased expression of OCT4 have been well documented in endometriosis. However, the molecular mechanism between TGF-β and OCT4 expression in endometriosis still remains largely unknown. In this study, we examined the roles of OCT4 in TGF-β-induced endometrial cell migration and the progression of human ectopic endometriosis. We analyzed the plasma levels of TGF-β (n = 80) and gene expression levels of TGF-βR1 in patient tissues (n = 60) by an ELISA assay and quantitative real-time RT-PCR. The serum TGF-β and tissue TGF-βR1 expressions were significantly increased in the ectopic endometriotic tissues (n = 54, 29 for adenomyosis tissues and 25 for chocolate cysts) comparing with the normal endometrium (n = 5). The TGFβR1 transcriptional level in endometriotic tissues was positively correlated with expressions of OCT4 and genes associated with cell migration, such as VIMENTIN, TWIST, SNAIL and SLUG. TGF-β dose-dependently increased the OCT4 expression both in gene and protein levels in human endometriotic stromal cells and, at a less intensity, in HEC1A endometrial carcinoma cell line. Additionally, TGF-βincreased the expression of migration-associated genes and proteins (SNAIL and N-CAD) dose-dependently both in gene and protein levels in human endometriotic stromal cells. Knockdown of OCT4 significantly suppressed the TGF-β-induced the expressions of migration-associated genes and proteins (N-CAD and SNAIL), and promoted the migration of endometrial cells as evidenced by wound-closure and transwell assays. Conclusions: TGF-β,TGF-βRI, and OCT4 are significantly upregulated in human ectopic endometriotic tissues. The significant OCT4 expression may be involved in the pathology of ectopic endometrial growth through TGF-β-induced migration of endometrial cells. Citation Format: Te-Sheng Chang, Heng-Kien Au, Thai-Yen Ling, Ching-Chi Chi, Chii-Ruey Tzeng, Jui-Hung Chang, Yen-Hua Huang. TGF-β1 enhanced cell migration involving pluripotent transcription factor OCT4 in endometriosis. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5580. doi:10.1158/1538-7445.AM2014-5580
Is Liver Biopsy Useful in the Evaluation of Patients with Chronically Elevated Liver Enzymes?
Authors: Michael M. Van Ness
Journal: Annals of Internal Medicine (1989)

Study Objective: To determine the diagnostic usefulness of percutaneous liver biopsy in evaluating patients with chronically elevated liver-associated enzymes. Design: Comparison of diagnosis made before biopsy by one physician on the basis of a noninvasive work-up (history, physical examination, laboratory values, and imaging studies) and final diagnosis made after biopsy by a second physician formulated after review of all available noninvasive information and study of the biopsy specimen. Setting: Referral-based gastroenterology clinic at a U.S. Navy medical center. Patients: Sequential sample of 107 patients with elevated liver-associated enzymes for a minimum of 3 months. Ninety patients were eligible for study. Interventions: The final diagnosis made by the second physician blinded to the first clinician's diagnosis served as the criterion standard. Measurements and Main Results: Four diagnostic groups were selected for analysis: Alcoholic liver disease, fatty liver, chronic necroinflammatory diseases, and miscellaneous. The positive predictive value of the prebiopsy diagnosis ranged from 88% (CI, 75% to 100%) for alcoholic liver disease to 56% (CI, 37% to 75%) for fatty liver. Higher elevations of transaminase values (greater than three times the upper limit of normal) correlated positively with increased prebiopsy diagnostic accuracy. Fatty liver was present in 19% of the cohort. Liver diseases requiring specific therapy other than alcohol abstinence were overlooked and diagnosed only after review of the biopsy in five cases. Conversely, four cases of liver disease, thought to require specific therapy on the basis of noninvasive work-up, were ruled out by biopsy. Conclusion: The cause of chronic liver disease is best elucidated when the noninvasive work-up is complemented by review of a biopsy specimen.
A CASE REPORT OF METASTATIC CARCINOMA TREATED WITH TEROPTERIN
Authors: M J KLAINER
Journal: Annals of Internal Medicine (1948)

Case Reports1 December 1948A CASE REPORT OF METASTATIC CARCINOMA TREATED WITH TEROPTERINMAX J. KLAINER, M.D., F.A.C.P.MAX J. KLAINER, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-29-6-1149 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptIn the years prior to the use of sulfonamides and more especially to the use of penicillin, infrequent reports of "cures" of individual cases of subacute bacterial endocarditis appeared in medical literature. As with subacute bacterial endocarditis in former years, metastatic carcinoma invariably has been fatal so that any therapy which gives a modicum of evidence of checking the spread of this fatal disease deserves consideration. It is, therefore, of interest to report a case of metastatic adenocarcinoma which apparently had been temporarily arrested and by the use of a new chemotherapeutic approach.CASE REPORTA 39 year old Armenian... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Stoneham, Massachusetts*Received for publication December 26, 1947.Teropterin is the name given by Lederle Laboratories Division, American Cyanamid Company, Pearl River, New York for pteroyl triglutamic acid. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byKlinische und histologische Beobachtungen bei der Chemotherapie maligner Tumoren 1 December 1948Volume 29, Issue 6Page: 1149-1157KeywordsAdenocarcinomaBacterial diseasesChildrenEndocarditisPenicillinRiversSulfonamide ePublished: 1 December 2008 Issue Published: 1 December 1948 PDF downloadLoading ...
DUODENAL LOOP CHANGES IN POSTERIOR PENETRATION OF DUODENAL ULCER
Authors: J G TEPLICK
Journal: Annals of Internal Medicine (1956)

Article1 May 1956DUODENAL LOOP CHANGES IN POSTERIOR PENETRATION OF DUODENAL ULCERJ. GEORGE TEPLICK, M.D.J. GEORGE TEPLICK, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-44-5-958 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe diagnosis and treatment of gastrointestinal disease is enormously influenced by the findings of the trained roentgenologist. In duodenal ulcer particularly, the clinician generally relies greatly on the roentgen findings. The ulcer complications of hemorrhage, obstruction and acute perforation frequently give rise to roentgen findings which are of decisive value in pinpointing the diagnosis.Chronic posterior perforation of a duodenal ulcer, though a fairly common occurrence, more often than not is first diagnosed on the surgical table. Only rarely does the roentgenologist suggest this diagnosis before surgery. For some time we have been interested in this ulcer complication, and have...Bibliography1. Bockus HL: Gastroenterology, Vol. I, 1946, W. B. Saunders Co., Philadelphia. Google Scholar2. Ibid., Vol. III. Google Scholar3. Poppel MH: Roentgen manifestations of pancreatic disease, 1951, Charles C. Thomas, Springfield, Illinois. Google Scholar4. GlennBaylin JCGJ: Roentgen findings in acute pancreatitis, Am. J. Roentgenol. 57: 604-615 (May) 1947. Google Scholar5. Case JT: Roentgenology of pancreatic disease, Am. J. Roentgenol. 44: 485-518, 1940. Google Scholar6. MethenyRobertsStranahan DEWA: Acute pancreatitis with special reference to x-ray diagnosis, Surg., Gynec. and Obst. 79: 504-508, 1944. Google Scholar7. GottliebDorfmanCregg CMHA: Pancreatitis: pre-operative diagnosis by gastrointestinal roentgenography, Radiology 56: 528-533, 1951. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Philadelphia, Pennsylvania*Received for publication October 26, 1955.From the Department of Radiology, Kensington Hospital, Philadelphia, Pennsylvania. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byDANGERS OF CORTICOTROPHINTHE DUODENAL LOOP 1 May 1956Volume 44, Issue 5Page: 958-974KeywordsChronic pancreatitisDiagnostic radiologyDuodenal ulcersGastroenterology and hepatologyHemorrhageIngestionPancreasSurgeryUlcers ePublished: 1 December 2008 Issue Published: 1 May 1956 PDF downloadLoading ...
Correlation of minor histocompatibility antigen-specific cytotoxic T lymphocytes with graft-versus-host disease status and analyses of tissue distribution of their target antigens
Authors: D Niederwieser, A Grassegger, J Aubock, M Herold, D Nachbaur, A Rosenmayr, A Gachter, W Nussbaumer, S Gaggl, M Ritter
Journal: Blood (1993)
Book: Sickened: The True Story of a Lost Childhood
Authors: Sean A Spence
Journal: BMJ (2004)

One variety of child abuse name-checked in the British media right now is Munchausen's syndrome by proxy (MBP): the infliction of suffering, symptoms, and signs upon a child by its “carer,” facilitating some need that the latter may have to interact with doctors. Children may be maimed and murdered by their kith and kin, and all in the pursuit of medical interventions. Raised in Ohio, in the American Midwest, Julie Gregory survived such an upbringing as the proxy for her mother, a truly damaged woman, who put her daughter through the mill.​mill.




Figure 1



Julie Gregory

Century, £12.99, pp 260 ISBN 1 844 13442 3

Rating: ★★★★




“The nurse at my feet says, `Now this might be a little stick, Julie. We've got to get this plastic tube into the urethra `cause Mom says you can't go.'

“My heart is pounding; what's urethra? What is she doing down there? I open my mouth to ask, but a startled scream rips out instead.”

Later, her mother will elicit the same ordeal for Julie's brother: “I heard him scream and whimper clear down the hall into the waiting room. Nobody had to tell me what was happening. He was getting a tube shoved into his urethra...”

This traipsing between credulous doctors (their notes are reproduced in the text) occurs beneath the lurking shadow of the American Gothic summoned up by Gregory: a place where fat fathers lounge in trailers, shouting for food that they may eat off their bloated bellies, while women who poison children coerce them into saying that they see Jesus, and men “do things” to children in bushes, while other adults “turn a blind eye.”

The book is compelling, not only because one wishes to see how the author will survive into adulthood, but also because the language is so often beautiful: “But the memories that hang heaviest are the easiest to recall. They hold in their creases the ability to change one's life, organically, forever. Even when you shake them out, they've left permanent wrinkles in the fabric of your soul.”

Of course, there is no easy escape. For the one who leaves such a home must worry over those who are left: younger siblings, children fostered, the vulnerable. Gregory conveys the sheer emptiness of carrying on alone, away from a place that is necessarily associated with suffering: “The more I acclimate to the normal world, the more and more surreal and unbelievable the world I came from seems.”

Eventually, she will find some release through learning of her condition, through reading works of literature, and by facing down her mother in full flight. The invocation of her inner voice may inspire the reader to stop and think: “Hey, you, with the frontal lobe, turn off the TV, stop the noise, and consider this deeply.”
Complete Remission of t(11;17) Positive Acute Promyelocytic Leukemia Induced by All-trans Retinoic Acid and Granulocyte Colony-Stimulating Factor
Authors: J.H. Jansen, M.C. de Ridder, W.M.C. Geertsma, C.A.J. Erpelinck, K. van Lom, E.M.E. Smit, R. Slater, B.A. vd Reijden, G.E. de Greef, P. Sonneveld, B. Löwenberg
Journal: Blood (1999)
486. Plasmid DNA Constructs Expressing SARS-CoV Antigens Are Immunogenic in Mice and Rabbits
Authors: Jong-Gu Park, Ik-Jae Moon
Journal: Molecular Therapy (2004)

A short peptide of the nuclear localization signal (NLS) of HIV-1 Tat protein, Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg, was modified by adding a specified amino acid at the NH2 and COOH termini. These peptides were simply added to DNA before being added with cationic liposomes to improve intracellular trafficking of the plasmid DNA. The Tat peptides were first mixed with reporter plasmid that express either luciferase, LacZ, or GFP, and then mixed with liposomes, to form the trimeric complex of DNA/peptide/liposome (DPL). The DPL complex was treated to various cell lines, and was compared for transfection efficiency with that obtained by the conventional DNA/liposomes complex. When tested for transfection efficiency employing peptides with end modifications, the peptide with COOH-terminal modification showed highest transfection efficiency. When the DPL complex was formed with various cationic liposomes, DOSPA/DOPE (LipofectamineTM) exhibited better transfection efficiency than other liposomes tested. Meanwhile plasmid DNA mixed only with the Tat NLS peptide showed significantly lower transfection when compared to that obtained with the DPL complex. When an optimal ratio of each DPL component was used with the DOSPA/DOPE liposomes, transfection efficiency was shown to be 100 to 600 fold better in reporter gene expression than that obtained with the DNA/liposomes (without peptide addition) complex. This study shows that the trimeric DPL complex can be simply formed and employed for enhanced expression of a transgene in many different cells with a strong potential for practical applications.
Abstract 15395: Urinary 8-Hydroxy-2'-Deoxyguanosine is a Biomarker for Evaluating the Inflammatory Activity and the Effectiveness to Corticosteroids in Patients with Cardiac Sarcoidosis
Authors: Takeo Tanaka, Shigeki Kobayashi, Takehisa Susa, Shinichi Okuda, Masahiro Doi, Yasuaki Wada, Jutaro Yamada, Takeshi Ueyama, Takayuki Okamura, Syuji Kawamura, Masafumi Yano, Masunori Matsuzaki
Journal: Circulation (2010)
A New Light upon Hendrik van Deventer (1651–1724): Identification and Recovery of a Portrait
Authors: R M F Van Der Weiden, W J Hoogsteder
Journal: Journal of the Royal Society of Medicine (1997)

textabstractEarly in 1996, both the artist and the patron of a beautifully
painted portrait by an anonymous painter of the late
seventeenth century were identified. The painting (Figure
1) was compared with works by other portraitists, and a
thorough stylistic analysis by the Netherlands Institute for
Art History finally showed that the portrait displays all the
characteristics of a painting by Thomas van der Wilt (1659-
1733)1. In the autumn of that year the painting was restored
and the signature and the date (1700) were recovered. This
proved that the maker of the portrait was indeed Thomas
van der Wilt.
A human IFNGR1 small deletion hotspot associated with dominant susceptibility to mycobacterial infection
Authors: Emmanuelle Jouanguy, Salma Lamhamedi-Cherradi, David Lammas, Susan E. Dorman, Marie-Claude Fondanèche, Stéphanie Dupuis, Rainer Döffinger, Frédéric Altare, John Girdlestone, Jean-François Emile, Henri Ducoulombier, David Edgar, Jane Clarke, Vivi-Anne Oxelius, Melchiorre Brai, Vas Novelli, Klaus Heyne, Alain Fischer, Steven M Holland, Dinakantha S Kumararatne, Robert D. Schreiber, Jean-Laurent Casanova
Journal: Nature Genetics (1999)
Abstract P007: Ongoing Resuscitation Research is Associated with Higher Odds of Receiving Bystander CPR
Authors: Pandora L Wander, Carol Fahrenbruch, Sofia Husain, Mickey Eisenberg, Thomas D Rea
Journal: Circulation (2014)

Introduction: The act of conducting a research study could potentially improve quality of care by focusing providers on the study condition or detract from care by distracting providers with complex protocols. We hypothesized that odds of receiving bystander cardiopulmonary resuscitation (CPR) would be higher during a trial of dispatcher-assisted CPR instructions than during periods immediately before and after. Methods: The investigation was a cohort study of 8,626 adult subjects who suffered non-traumatic out-of-hospital cardiac arrest prior to emergency medical services (EMS) arrival in greater King County, Washington, between January 1, 1999, and December 31, 2011. Bystander CPR status was assessed through review of audio dispatch tapes and EMS reports to classify any bystander CPR (any B-CPR). Any B-CPR was further categorized as bystander CPR with dispatcher assistance (DA-CPR) or bystander CPR without dispatcher assistance (B-CPR, no DA). We used multivariable logistic regression to evaluate the odds of any B-CPR before, during, and after the Dispatcher Assisted Resuscitation Trial (DART), a randomized trial of dispatcher-assisted CPR instruction comparing chest compressions alone with compressions plus rescue breaths, which ran from June 1, 2004, to September 30, 2009. We also evaluated whether the odds varied by type-specific B-CPR. Results: Patient and arrest circumstances were similar across the study periods. Compared to the period before DART, odds of receiving any B-CPR were higher during DART (OR=1.35, 95% CI = 1.23-1.49), but no different after OR=1.11, 0.98-1.25). Similarly, compared to the before period, the odds of DA-CPR were higher during DART (OR=1.79, 1.59-2.02) but no different after (OR=0.94, 0.80-1.10) (Table 1). Discussion: Odds of bystander CPR were higher during the study compared to periods before and after. The increase seems to be largely related to higher likelihood of DA-CPR, suggesting a possible community-wide benefit from this research protocol.