tbuckley/cabot-search huggingface dataset:
PAR, a putative chromosomal passenger protein involved in cell cycle
Authors: Desmond N. Jackson, Alin Ionescu, Elena Ivan, James F. Holland, Micsumica Platica, Ovidiu Platica
Journal: Cancer Research (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

4910 

Recently we reported the isolation of a novel gene called PAR, which has been shown to be involved in proliferation of normal and tumor cells. The gene is ubiquitously expressed in normal cells and is overexpressed in most of their malignant counterparts. The ectopic overexpression of PAR induced malignant phenotype in NIH3T3 cells. Here we present our data on the role of this gene in cell cycle. The PAR level of expression during the cell cycle was studied using Western blots and flow cytometry. In DU145 cells synchronized by double thymidine incorporation in G1/S phase PAR level was low in G1/S phase, increased as the cells progressed through S phase, reached the maximum in G2/M phase and returned to low levels when the cells re entered the G1 phase. The presence of 2 D box sequences at the amino and carboxy terminus of PAR molecule suggests a role for ubiquitination/ proteasome degradation at the end of the mitosis. The localization of PAR protein during mitosis was studied by immunofluorescence using and antiPAR antibody. In prophase and metaphase PAR protein is localized in the centrosome and in the spindle poles. Then, in anaphase PAR translocates to the spindle midzone. At late ana/telophase and cytokinesis PAR accumulates in the midbody. During mitosis, a fraction of PAR protein is distributed around the cell periphery. In addition, after the onset of anaphase a portion of PAR is closely associated with the cell membrane, at the cleavage furrow. This localization during cell cycle suggests that PAR might be related with chromosomal passenger proteins (CPP). This is also supported by confocal microscopy studies showing that PAR co-localizes with Aurora at centrosomes and with Survivin at the midbody. Furthermore, immunoprecipitation with anti PAR Ab indicated that PAR forms complexes with Aurora and Survivin. PAR, like the other CPP, could be involved in various aspects of the cell cycle, including centrosomes segregation, spindle formation, and cytokinesis, which could become aberrant when PAR expression changes. Transfection of DU145 cells with PAR antisense oligonucleotides resulted in defects at multiple stages of the cell cycle: increased polyploidy, cells with multipolar spindles and aberrant mitosis. Further work is pursued in our laboratory to expand these findings and to get more information about the function and mechanism of PAR action. Evaluating the amplification and expression of PAR in a range of human tumors will further define its contribution to tumor development. If these studies will confirm our previous findings on PAR over expression in tumors, this protein could be used as a new marker for malignant cell phenotype. In addition, PAR could become a potential target for the treatment of different cancers.
Safety and Cost-Effectiveness of Early Discharge After Primary Angioplasty in Low Risk Patients With Acute Myocardial Infarction 11Research funding for this study was provided by unrestricted grants from Advanced Cardiovascular Systems, Santa Clara, California; Mallinckrodt Medical, Inc., Saint Louis, Missouri; Datascope Corporation, Montvale, New Jersey; St. Jude Medical, Chelmsford, Massachusetts; and Siemens Corporation, Iselin, New Jersey.
Authors: Cindy L. Grines, Dominic L. Marsalese, Bruce Brodie, John Griffin, Bryan Donohue, Costantino R. Costantini, Carlos Balestrini, Gregg Stone, Thomas Wharton, Paolo Esente, Michael Spain, Jeffrey Moses, Masakiyo Nobuyoshi, Mike Ayres, Denise Jones, Denise Mason, Debra Sachs, Lorelei L. Grines, William O’Neill
Journal: Journal of the American College of Cardiology (1998)
Transcriptomics from single cells
Authors: Rashel Grindberg, Roger S. Lasken
Journal: Clinical Cancer Research (2010)

Abstract New technologies are making it possible to measure gene expression from single cells. Reverse transcriptase is used to convert the few picograms of mRNA in a cell to cDNA. Next generation sequencing methods, such as SOLiD sequencing, provide a low cost means to sequence the cDNA allowing quantification as well as genotyping. Unlike RT-PCR which enables single cell measurement of one or a limited number of transcripts, the entire transcriptome can be accessed. It will be possible to discover new correlations between genes and their role in diseases. We have begun testing this method on a variety of cell types including cultured cancer cells and human stem cells. Methods are in development to test circulating cancer cells. Progress has also been made on a different technology that addresses genotyping of the genomic DNA from one cell. Whole genome amplification by a method called Multiple Displacement Amplification (MDA) provides sufficient DNA for use in sequencing and genotyping. This enables analysis of unexpressed regions of the genome which are lost in transcriptional studies. Together, single cell methods for transcriptomics and genotyping of the genomic DNA will be useful in studying disease processes at the level of the individual cell. It will be possible to isolated specific cell types based on their phenotypic characteristics and then determine their genetic and transcriptional signatures.
Mutation analysis of Chinese sporadic congenital sideroblastic anemia by targeted capture sequencing
Authors: Wenbin An, Jingliao Zhang, Lixian Chang, Yingchi Zhang, Yang Wan, Yuanyuan Ren, Deyun Niu, Jian Wu, Xiaofan Zhu, Ye Guo
Journal: Journal of Hematology & Oncology (2015)

Congenital sideroblastic anemias (CSAs) comprise a group of heterogenous genetic diseases that are caused by the mutation of various genes involved in heme biosynthesis, iron-sulfur cluster biogenesis, or mitochondrial solute transport or metabolism. However, approximately 40 % of patients with CSA have not been found to have pathogenic gene mutations. In this study, we systematically analyzed the mutation profile in 10 Chinese patients with sporadic CSA. We performed targeted deep sequencing analysis in ten patients with CSA using a panel of 417 genes that included known CSA-related genes. Mitochondrial genomes were analyzed using next-generation sequencing with a mitochondria enrichment kit and the HiSeq2000 sequencing platform. The results were confirmed by Sanger sequencing. The ALAS2 mutation was detected in one patient. SLC25A38 mutations were detected in three patients, including three novel mutations. Mitochondrial DNA deletions were detected in two patients. No disease-causing mutations were detected in four patients. To our knowledge, the pyridoxine-effective mutation C471Y of ALAS2, the compound heterozygous mutation W87X, I143Pfs146X, and the homozygous mutation R134C of SLC25A38 were found for the first time. Our findings add to the number of reported cases of this rare disease and to the CSA pathogenic mutation database. Our findings expand the phenotypic profile of mitochondrial DNA deletion mutations. This work also demonstrates the application of a congenital blood disease assay and targeted capture sequencing for the genetic screening analysis and diagnosis of heterogenous genetic CSA.
Early discharge after surgery for breast cancer
Authors: D. Harcourt, S. Cawthorn, N. Ambler, N. Rumsey
Journal: BMJ (1999)

EDITOR—We are pleased that psychological implications of early discharge after surgery for breast cancer are being examined1 but think that some important aspects of the policy have been inappropriately or inadequately researched and warrant further investigation.

There is a drawback to applying randomised controlled trial methodology in situations such as this, where patients are making choices about their care. A self selecting process operates and must be taken into …
Abstract 16590: Late Myocardial Infarction After Fontan in Pulmonary Atresia With Intact Ventricular Septum
Authors: Adam J Small, Yuli Y Kim, Andrew C Glatz, Kevin K Whitehead, Kiona Y Allen, Tacy E Downing, Richard Donner, Stephanie Fuller, Therese M Giglia
Journal: Circulation (2014)

Background:  Right ventricle (RV)-to-coronary fistulae, coronary stenoses, and atresia are common in pulmonary atresia with intact ventricular septum (PA-IVS). Myocardial infarction (MI) and sudden death after RV decompression in the neonate have been associated with coronary anomalies that create a RV-dependent coronary circulation. There is a paucity of data, however, on long-term coronary sequelae after Fontan completion.   Methods:  A retrospective review of patients followed at our institutions who underwent Fontan for PA-IVS between 1985 and 2013 was performed. Patients with antegrade pulmonary blood flow were excluded. Charts were reviewed for sudden death as well as for clinical, radiologic, and angiographic evidence of myocardial infarction/ischemia.   Results:  Forty-six patients met inclusion criteria with a median age at Fontan of 2.6 (range 1.2 - 18.9) years. Median follow-up from Fontan was 3.5 (range 0 - 27.9) years. Twenty-three patients (50%) had adequate angiograms for review and of those, 18 (75%) had RV-to-coronary fistulae; 13 (54%) had fistulae with coronary stenoses; and 2 (8.3%) had aortocoronary atresia. There were 2 early deaths (4.3%) but neither had angiograms nor non-invasive tests of myocardial perfusion for review. Five patients (11%) had evidence of ischemia. Of those, 2 (4.3%) had non-fatal MI at 16.9 and 18.8 years post-Fontan. Both patients had fistulae and one had severe mid-right coronary stenosis. Coronary anomalies correlated with ischemic territory on non-invasive testing in both. Three additional patients had evidence of ischemia on stress test and/or cardiac MRI without clinical evidence of MI. Two of those had angiograms, both with fistulae and coronary stenoses that correlated with ischemic territory on non-invasive testing. No patient had traditional risk factors for atherosclerotic coronary disease. No patient underwent coronary revascularization. There was no sustained ventricular tachycardia or sudden death. There were no late deaths.   Conclusion:  Patients with PA-IVS and single-ventricle palliation remain at risk for long-term myocardial ischemia. Further study is necessary to define risk factors for ischemic outcomes.
Using Telehealth to Enable Patient-Centered Care for Liver Transplantation
Authors: Audrey E. Ertel, Tiffany Kaiser, Shimul A. Shah
Journal: JAMA Surgery (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal
584. Intratumoral Electrotransfer of Interleukin-12 Gene and Bleomycin Synergistically Induced Therapeutic Immunity and Suppressed Subcutaneous and Metastatic Melanomas in Mice
Authors: Tsunao Kishida, Hidetsugu Asada, Hiroshi Nakano, Kakei S. Yasutomi, Masaharu Shin-ya, Takashi Shinomiya, Feng-De Cui, Yasuo Hisa, Jiro Imanishi, Osam Mazda
Journal: Molecular Therapy (2004)

In vivo electroporation-mediated transfer of chemotherapeutic agents provides an effective means for treatment of neoplasms, as demonstrated by clinical trials against various tumors including malignant melanoma. We postulated that the combination of the electro-chemotherapy and intratumoral cytokine gene therapy may further improve the therapeutic efficacy, thanks to the anti-tumor immune responses that may eradicate not only the manipulated tumors but also distant metastatic lesions.
Peritoneal Dialysis for Psoriasis
Authors: Z. J. TWARDOWSKI
Journal: Annals of Internal Medicine (1978)

Remissions of long-standing psoriasis have been reported in patients starting either chronic hemodialysis or peritoneal dialysis for renal failure. To see if dialysis influences the course of psoriasis in the absence of renal failure, we selected three patients with severe, refractory, long-standing psoriasis to undergo weekly peritoneal dialysis treatments. Two began to improve after the first dialysis, with nearly complete resolution after four and nine treatments, respectively. The third patient showed no objective changes after four dialyses. These findings add to increasing anecdotal reports of psoriasis improving with dialysis and extend the observations to patients without renal disease.
Abstract 9848: A Subgroup Analysis of Carotid Artery Stenting with Distal Protection in High Surgical Risk Patients in Routine Clinical Practice: Results of the CABANA Safety Surveillance Program
Authors: Christopher J White, L. Nelson Hopkins, Malcolm Foster, Richard J Powell, Gerald Zemel, Juan Diaz-Cartelle, Keith D Dawkins
Journal: Circulation (2011)

INTRODUCTION: The multicenter, nonrandomized CABANA Study evaluated periprocedural outcomes in high surgical risk patients with carotid artery stenosis treated with the Carotid WALLSTENT plus FilterWire EZ Embolic Protection System (Boston Scientific, Natick, MA) HYPOTHESIS: We assessed whether a patient's symptomatic status, high risk category and age (≤80 years vs &gt;80 years) had an impact on the 30-day composite rate and individual event rates of major adverse events (MAE including stroke, death and myocardial infarction [MI]). METHODS: Of the 1,097 patients enrolled, 34.4% were at high risk for carotid endarterectomy due to prespecified anatomic criteria, while 65.6% had medical comorbidities. Approximately one-third of patients were symptomatic for carotid artery disease (≥50% carotid artery stenosis); the rest were asymptomatic (≥80% carotid stenosis). Follow-up at 30 days included clinical evaluation and independent neurologic and NIH stroke scale assessments. RESULTS: At 30 days postprocedure, the MAE rate in the overall population was 4.6% (47/1025). In asymptomatic and symptomatic patients, MAE rates were 4.2% (29/697) and 5.5% (18/328); respectively ( P =0.34); in the ≤80-year and &gt;80-year cohort, MAE rates were 4.1% (34/836) and 6.9% (13/189), respectively ( P =0.10). Individual event rates are shown in Table 1. Patients with comorbid risk factors showed strong trends towards higher major stroke rates (2.5% vs 0.9%, P =0.07) versus those at anatomic high risk. No differences were seen in MAE (3.7%, anatomic vs 5.1%, comorbid, P=0.33), death, or MI rates between groups. CONCLUSIONS: Composite MAE and individual event rates at 30 days in all subgroups were low and similar to the outcomes in the overall CABANA dataset. A trend toward higher incidence of major stroke in the comorbid high risk group was observed. These results demonstrate the safety of the Carotid WALLSTENT plus FilterWire EZ in high surgical risk patients, regardless of their risk profile.
Abstract 4301: Detection and characterization of regions of hypoxia within orthotopic pancreatic tumors using multispectral optoacoustic tomography
Authors: Shanice V. Hudson, Charles Kimbrough, Michael Egger, Anil Khanal, Michelle E. Smith, William E. Grizzle, Lacey R. McNally
Journal: Cancer Research (2014)

Abstract Optical imaging is a vital tool in cancer research for visualization of tumors in preclinical models. However, resolution for bioluminescence and fluorescence modalities is restricted to a depth of five millimeters, as light is attenuated at increased depth and autofluorescence can create background noise. Light propagation becomes diffuse at only a few millimeters, resulting in light scattering and absorption. To improve imaging resolution and acquisition in real-time, multispectral optoacoustic tomography (MSOT) was utilized to visualize molecular phenomena such as the tissue oxygenation of characteristically hypoxic aggressive pancreatic tumors. SCID mice were orthotopically implanted with S2VP10L and MIA PaCa-2 pancreatic cancer cells that highly express the epidermal growth factor receptor (EGFR), which exhibits extracellular domain binding of the epidermal growth factor (EGF) ligand. Cohorts were then intravenously injected with fluorescent EGF ligand probes and imaged semiweekly to follow tumor growth and morphology. Medical Grade Air levels were set to 0.9 L and oxygen at 0.1 L to assess the correlation of increased hypoxia with tumor growth and progression. Pancreatic tumor cells were imaged concurrently with evaluating levels of oxygenated and deoxygenated hemoglobin, providing a distinct picture of the tumor and its vascularization. Use of MSOT to detect regions of hypoxia within tumor was validated using pimonidazole immunohistochemical staining of tumor tissue. For the first time, our data demonstrate regions of tumor hypoxia in living subjects, at depth, and noninvasively, via optoacoustic tomography. Dynamic assessment of tumor microenvironment, especially regions of tumor hypoxia, is essential to effectively target and treat tumors that are otherwise resistant to treatment. Citation Format: Shanice V. Hudson, Charles Kimbrough, Michael Egger, Anil Khanal, Michelle E. Smith, William E. Grizzle, Lacey R. McNally. Detection and characterization of regions of hypoxia within orthotopic pancreatic tumors using multispectral optoacoustic tomography. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4301. doi:10.1158/1538-7445.AM2014-4301
Mammalian target of rapamycin (mTOR) inhibitors slow skin carcinogenesis, but impair wound healing
Authors: L. Feldmeyer, G.F.L. Hofbauer, T. Böni, L.E. French, J. Hafner
Journal: British Journal of Dermatology (2011)

Recent studies suggest that patients on mammalian target of rapamycin (mTOR) inhibitors experience a reduction in cutaneous carcinogenesis by an estimated 50% or more compared with calcineurin inhibitors. While randomized trials are running, organ transplant recipients are frequently switched from calcineurin inhibitors to mTOR inhibitors when cutaneous carcinogenesis increases.To slow carcinogenesis in our patient, a heart transplant recipient with a neuropathic diabetic foot syndrome who had developed cutaneous carcinogenesis at a rate of more than 20 squamous cell carcinomas (SCC) annually.The patient's immunosuppression was switched from the calcineurin inhibitor ciclosporin to the mTOR inhibitor everolimus.Carcinogenesis slowed to six SCC annually; however, he developed recalcitrant diabetic foot ulcers which were purely neuropathic and nonangiopathic, and a limb-threatening fistulating necrotic erysipelas of the right leg. Both sites responded poorly to antibiotic therapy, offloading and debridement. This skin fistula became chronic and some toes were at risk for minor amputation. In view of the propensity for mTOR inhibitors to impair would healing, immunosuppression was switched back to ciclosporin. All wounds healed rapidly, but skin carcinogenesis rose to former levels.This case impressively illustrates the clinical dilemma for mTOR inhibitor use where benefit in carcinogenesis is counterbalanced by impairment in wound healing. Changes in immunosuppressive regimens should thus be made on an individual basis with careful consideration of the relative risks.
THE TUBERCULOSIS OF CHILDHOOD
Authors: Charles Hendee Smith
Journal: Annals of Internal Medicine (1935)

Article1 March 1935THE TUBERCULOSIS OF CHILDHOODCHARLES HENDEE SMITHCHARLES HENDEE SMITHSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-8-9-1090 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTuberculosis in childhood has many clinical manifestations. It can assume many forms which are seen predominantly in children and in addition many others seen in adult life. Only in recent years has its importance been realized, since the difficulties in diagnosis, and the insidious and occult forms in which it occurs have made it easily overlooked. There are many prevalent misconceptions about the disease, and several misleading pseudodescriptive terms are imbedded in the terminology. Many writers on the subject are students of adult tuberculosis, pathologists or radiologists. This paper presents the point of view of the pediatrician, in the light... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: New York, N. Y.*Read at the Chicago meeting of the American College of Physicians, April 16, 1934.From the Department of Pediatrics, New York University, and The Children's Medical Service and Pathological Laboratory of Bellevue Hospital. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byTreatment of Tuberculosis in Childhood 1 March 1935Volume 8, Issue 9Page: 1090-1120KeywordsChildrenMedical servicesPathologistsPediatricsRadiologistsResearch laboratories ePublished: 1 December 2008 Issue Published: 1 March 1935 PDF downloadLoading ...