tbuckley/cabot-search huggingface dataset:
Differential proteomics of metastatic and non-metastatic lung carcinoma cell lines
Authors: Cecile Paboeuf, Hongtao Qi, Cristina Draghici, Yasuo Konishi, Robert Menard, Edmund Ziomek
Journal: Cancer Research (2006)

4548   Two lung carcinoma cell lines, CL1-0 and CL1-5 [Chu, Y. W. et al. Am. J. Respir.Cell. Mol. Biol.,  17:  353-360, 1997] are different in their invasive and metastatic potential. Both cell lines form tumors when injected to mice, but only CL1-5 cells lead to tumors capable of metastasizing to lungs. For the purpose of this work we used subcellular fractions obtained from CL1-0 and CL1-5 cells. Proteins in the cytosol were labeled with two different fluorescent dyes. We chose Alexa Fluor 555 and Alexa Fluor 647 C 2  maleimide derivatives (Invitrogen) for their stability, similar molecular weight and affordability. The cytosol proteins were labeled with the excess of the dye - each sample with a different dye. Equal amounts of labeled CL1-0 and CL1-5 cytosols were mixed and proteins resolved by 2D-gel electrophoresis. The 2D-gels were scanned (Typhoon, GE Healthcare) and protein spots belonging to either CL1-0, or to CL1-5 were identified. In addition to the fluorescence overlay image, proteins were visualized by silver staining, extracted from the gel and subjected to in-gel tryptic digestion and LC-MS/MS analysis. To verify reproducibility of labeling and separation by 2-D PAGE we inversed the labeling and repeated the proteomic analysis. Due to a higher molecular weight of Alexa Fluor 647 C 2  maleimide (approx.50 Da), saturation labeling of low-molecular-weight proteins resulted in a small, but easy to recognize shift of the spots on the overlay image. Importantly, isoelectric point was the same for proteins labeled either with Alexa Fluor 555, or Alexa Fluor 647. In both experiments we observed the same unique spots. Unique protein spots were observed mainly in CL1-0 and absent, or decreased in CL1-5 cytosol samples, i.e., down-regulated in more metastatic carcinoma cell line. Among the proteins down-regulated in CL1-5 cells were annexin I (ANX1), heat shock protein 27 (HSP 27) and down-regulated ovarian cancer 1 (DOC1), all three already established cancer markers. Somewhat unexpected was identification of the biliverdin IX beta reductase (AAB29537), a protein shown to have an antiapoptotic activity. Western blot analysis confirmed a high level of HSP27 and ANX1 in cytosols from CL1-0 cells, and a much lower signal for these proteins was observed in CL1-5 cells. In overall, we have demonstrated that Alexa Fluor dyes can be used successfully in the differential gel electrophoresis and subsequent proteomic identification of the cancer protein markers.
Abstract 430: LXR Activation Reduces Expression of Renin, ACE, and Angiotensin Receptor Type 1 in Kidney and Heart
Authors: Irma Kuipers, Linda van Genne, Dirk J van Veldhuisen, Wiek H van Gilst, Rudolf A de Boer
Journal: Circulation (2006)

Background: Liver X receptor (LXR)-alpha is a pivotal player in the reverse cholesterol metabolism, which stimulates cholesterol efflux from tissues and which has been implicated as an endogenous a...
Miss Juliana Willoughby
Authors: Juliana M. Walker
Journal: JAMA (2001)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Effect of Ebola Progression in Liberia
Authors: Harry B. Burke
Journal: Annals of Internal Medicine (2015)

Letters19 May 2015Effect of Ebola Progression in LiberiaHarry B. Burke, MD, PhDHarry B. Burke, MD, PhDFrom Uniformed Services University of the Health Sciences, Bethesda, Maryland.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/L15-5092-2 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:Case isolation has been the method of choice for dealing with disease epidemics for hundreds of years. Yamin and colleagues (1) improve on our knowledge of how to truncate the human–human transmission of a communicable disease by estimating the time during which case isolation is most effective for patients with Ebola, namely, within 4 days of symptom onset. Because rapidly fatal diseases for which no effective treatment is available tend to be self-limiting, can the authors estimate how long the Ebola pandemic will continue before it dies out on its own?Harry B. Burke, MD, PhDUniformed Services ...Reference1. Yamin D, Gertler S, Ndeffo-Mbah ML, Skrip LA, Fallah M, Nyenswah TG, et al. Effect of Ebola progression on transmission and control in Liberia. Ann Intern Med. 2015;162:11-7. [PMID: 25347321] doi:10.7326/M14-2255 LinkGoogle Scholar Author, Article, and Disclosure InformationAuthors: Harry B. Burke, MD, PhDAffiliations: From Uniformed Services University of the Health Sciences, Bethesda, Maryland.Disclosures: Authors have disclosed no conflicts of interest. Forms can be viewed at www.acponline.org/authors/icmje/ConflictOfInterest Forms.do?msNum=L15-0103. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoEffect of Ebola Progression on Transmission and Control in Liberia Dan Yamin , Shai Gertler , Martial L. Ndeffo-Mbah , Laura A. Skrip , Mosoka Fallah , Tolbert G. Nyenswah , Frederick L. Altice , and Alison P. Galvani Effect of Ebola Progression on Transmission and Control in Liberia Dan Yamin , Shai Gertler , Martial L. Ndeffo-Mbah , Laura A. Skrip , Mosoka Fallah , Tolbert G. Nyenswah , Frederick L. Altice , and Alison P. Galvani Effect of Ebola Progression in Liberia Prasanta Raghab Mohapatra and Baijayantimala Mishra Effect of Ebola Progression in Liberia Dan Yamin , Shai Gertler , Martial L. Ndeffo-Mbah , and Alison P. Galvani Effect of Ebola Progression in Liberia Dan Yamin , Shai Gertler , Martial L. Ndeffo-Mbah , and Alison P. Galvani Effect of Ebola Progression in Liberia Prasanta Raghab Mohapatra and Baijayantimala Mishra Metrics 19 May 2015Volume 162, Issue 10Page: 734KeywordsConflicts of interestDisclosure ePublished: 19 May 2015 Issue Published: 19 May 2015 Copyright & PermissionsCopyright © 2015 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...
Oxygen saturation and perfusion changes during photodynamic therapy: use of noninvasive monitoring
Authors: S. Ibbotson
Journal: British Journal of Dermatology (2011)

Conflicts of interest S. Ibbotson has received travel expenses from Galderma, U.K. Photodynamic therapy (PDT) requires tissue‐localized photosensitizer, light of appropriate wavelength and the presence of oxygen. Indeed, the manipulation of oxygen tension can alter the efficacy of PDT in vitro, resulting in opportunities for optimization of treatment.1 The use of topical PDT in dermatology for dysplasia and superficial nonmelanoma skin cancer is well established,2 and its routine use in clinical practice has advanced beyond our understanding of what is happening at a cellular level in human skin. While topical PDT is highly effective in selected patients, relapse rates of approximately 20% can be expected by the 5‐year follow‐up.3 In addition, current PDT regimens are not maximally effective for lesions such as thicker nodular basal cell carcinomas, and pain during irradiation can sometimes be limiting. In view of this, there is a clinical need for optimization of PDT treatment parameters, and one approach has been to maximize PDT efficiency based on in vivo data,4 such as the use of lower irradiance or pulsed‐light delivery.
Expression and Function of Aminopeptidase N/CD13 Produced by Fibroblast‐like Synoviocytes in Rheumatoid Arthritis: Role of CD13 in Chemotaxis of Cytokine‐Activated T Cells Independent of Enzymatic Activity
Authors: Rachel Morgan, Judith Endres, Nilofar Behbahani‐Nejad, Kristine Phillips, Jeffrey H. Ruth, Sean C. Friday, Gautam Edhayan, Thomas Lanigan, Andrew Urquhart, Kevin C. Chung, David A. Fox
Journal: Arthritis & Rheumatology (2014)

Objective Aminopeptidase N/CD13 (EC 3.4.11.2) is a metalloproteinase expressed by fibroblast‐like synoviocytes (FLS). It has been suggested that CD13 can act chemotactically for T cells in rheumatoid arthritis (RA). We undertook this study to measure CD13 in vivo and in vitro in RA samples and to determine whether CD13 could play a role in the homing of T cells to the RA joint. Methods Interleukin‐17–treated FLS were used to immunize mice, from which a novel anti‐human CD13 monoclonal antibody (mAb), 591.1D7.34, was developed. The mAb 591.1D7.34 and a second anti‐CD13 mAb, WM15, were used to develop a novel enzyme‐linked immunosorbent assay (ELISA) for CD13, and CD13 enzymatic activity was measured in parallel. Chemotaxis of cytokine‐activated T cells was measured by a chemotaxis‐under‐agarose assay. Results We detected substantial amounts of CD13 in synovial fluid (SF), sera, FLS lysates, and culture supernatants by ELISA, with a significant increase in CD13 in RA SF when compared to osteoarthritis SF. CD13 accounted for most but not all of the CD13‐like enzymatic activity in SF. Recombinant human CD13 was chemotactic for cytokine‐activated T cells through a G protein–coupled receptor and contributed to the chemotactic properties of SF independently of enzymatic activity. Conclusion CD13 is released from FLS into culture supernatants and is found in SF. CD13 induces chemotaxis of cytokine‐activated T cells, a T cell population similar to that found in RA synovium. These data suggest that CD13 could play an important role as a T cell chemoattractant, in a positive feedback loop that contributes to RA synovitis.
Persistence of cerebral metabolic abnormalities in chronic schizophrenia as determined by positron emission tomography
Authors: A Wolkin, J Jaeger, J D Brodie, A P Wolf, J Fowler, J Rotrosen, F Gomez-Mont, R Cancro
Journal: American Journal of Psychiatry (1985)

Local cerebral metabolic rates were determined by positron emission tomography and the deoxyglucose method in a group of 10 chronic schizophrenic subjects before and after somatic treatment and in eight normal subjects. Before treatment, schizophrenic subjects had markedly lower absolute metabolic activity than did normal controls in both frontal and temporal regions and a trend toward relative hyperactivity in the basal ganglia area. After treatment, their metabolic rates approached those seen in normal subjects in nearly all regions except frontal. Persistence of diminished frontal metabolism was manifested as significant relative hypofrontality. These findings suggest specific loci of aberrant cerebral functioning in chronic schizophrenia and the utility of positron emission tomography in characterizing these abnormalities.
Abstract P411: Associations Between Dietary Polyunsaturated Fatty Acid Intakes And Serum C-reactive Protein Concentrations In Adults From Nhanes 2003-10
Authors: Sam Sun, Brent Flickinger
Journal: Circulation (2014)

Background: Multiple clinical studies and literature reviews suggest that elevated serum C-reactive protein (CRP) concentration may be associated with risks of cardiovascular diseases (CVD), type-2 diabetes, certain autoimmune disorders, and infection or inflammation. Data are very limited and inconsistent about impact of dietary polyunsaturated fatty acid (PUFA) intakes on CRP concentrations. Method: PUFA intakes and CRP measurement in adults (aged 19 years and elder) were extracted from databases of NHANES 2003-10. Non-normally distributed data were normalized by log-transformation. SAS survey-mean and survey-regression procedures were used to analyze the dataset extracted. Result: In total, 17,785 participants who had valid CRP and PUFA intake data were included in this work. Means of PUFA intake, CRP concentration, and n6- to n3- fatty acids (FA) ratio were 15.11 g/d (median 15.56 and SE 0.07), 1.79 mg/L (median 1.80, SE 0.006), and 9.81 (median 9.21, SE 0.027), respectively. 38.94% (n=6925) participants had CRP concentration ≥3 mg/L with mean PUFA intake 14.36 g/d, 9.54% (n=1,696) ≥10~&gt;30 mg/L with mean PUFA intake 14.41 g/d, and 1.43% (n=254) ≥30mg/L with mean PUFA intake 12.91 g/d. From regression analyses, CRP concentration was significantly and inversely correlated with PUFA intake (P&lt;0.0001) with adjustment for saturated fat intake, gender, age, and BMI, but not correlated with the ratio of n6 to n3 FA (P=0.57). CRP concentration was also significantly and inversely correlated with PUFA intake quartiles (P=&lt;0.0001) with the same adjustment setting. Least square (LS) mean of CRP concentration in the lowest quartile group (2.06 mg/L) was significantly higher (P= 0.001~&lt;0.0001) than the LS means of all other 3 PUFA intake quartile groups. LS means of CRP concentration in the 2nd lowest and the 3 rd quartile groups (1.85 and 1.79 mg/L) were also significantly higher (P=0.005 and 0.046, respectively) than the LS mean of the highest quartile group (1.69 mg/L). Conclusion: Descriptive statistics and multi-covariate regression showed that dietary PUFA intakes were inversely associated with serum CRP concentration in the adult population, but not associated with n6- to n3- FA ratio. This finding is consistent with previous suggestions that sufficient PUFA or n3 FA intakes may enhance immune function, reduce risk of infection, and infection/inflammation-related diseases, such as CVD. In the population analyzed, intakes of linoleic acid, alpha-linolenic acid, n3 FA, n6 FA, and total PUFA were tightly correlated with each other (r=0.833-0.999, Spearman).
797. A Trans-Dominant Negative and Replication Defective HSV-1 Vector for In Vivo and Ex Vivo Gene Transfer
Authors: Christoph F. P. Theopold, Daniela Hoeller, Feng Yao
Journal: Molecular Therapy (2004)

HSV replicates in epithelial cells and establishes life-long latent infection in neuronal cell bodies of the sensory ganglia. This dual life cycle, and its ability to package 30-50kb of transgene, make it an attractive vehicle for gene transfer. However, both replication-defective and neuroattenuated viruses are replication competent in the context of wild-type virus, raising safety concerns about their use for human gene therapy.
Antiproliferative effects of anthocyanins, hydrolyzable and oligomeric tannins rich pomegranate fruit extract on human lung carcinoma cells A549
Authors: Naghma Hadi, Farrukh Afaq, Hasan Mukhtar
Journal: Cancer Research (2005)

Proc Amer Assoc Cancer Res, Volume 46, 2005

2467 

Lung cancer is the leading cause of cancer-related mortality in the United States. One approach to control this malignancy is its preventive intervention by dietary agents. Several naturally occurring compounds, especially plant products and dietary constituents, have been found to exhibit chemopreventive as well as chemotherapeutic activities when assessed under in vitro and in vivo model systems for lung cancer. However, search for an ideal cancer chemopreventive agent for lung cancer continues. Pomegranate ( Punica granatum ), a fruit native to the Mediterranean region has been used extensively in the folk medicine. We extracted edible seeds of pomegranate fruit with acetone, hereafter referred to as pomegranate fruit extract (PFE). Based on MALDI-TOF MS analysis PFE was found to contain anthocyanins (such as delphinidin, cyanidin, and pelargonidin), hydrolyzable tannins (such as punicalin, pedunculagin and punicalagin) and oligomeric ellagitannins in which two to five glucose units are crosslinked by dehydrodigalloyl units. Studies have shown that PFE possesses strong antioxidant and anti-inflammatory properties. Recently, we have shown that PFE possesses remarkable anti-tumor promoting effects in mouse skin model of chemical carcinogenesis (Afaq et al., Int J Cancer, 113:423-433, 2005). In the present study, employing normal human bronchial epithelial cells (NHBE), and human lung carcinoma A549 cells, we first compared the growth-inhibitory effects of PFE. PFE (50-150 μg/ml) treatment (for 72 hrs) was found to result in a dose-dependent decrease in the viability of A549 cells but had only minimal effects on NHBE cells as assessed by MTT and Trypan blue assay. To assess the antioxidant capacity of PFE in A549 cells, trolox antioxidant assay was performed and we found that PFE exhibited excellent antioxidant activity even higher than that of (-)-epigallocatechin-3-gallate, the major polyphenol present in green tea. PFE treatment (50-150 μg/ml for 72 hrs) to A549 cells resulted in induction of apoptosis as determined by fluorescence microscopy and flow cytometry. The immunoblot analysis revealed that PFE treatment resulted in a dose-dependent (i) induction of WAF1/p21 and KIP1/p27, (ii) decrease in the protein expressions of cyclin D1, cyclin D2, and cyclin E, and (iii) decrease in the protein expressions of cdk2, cdk4, and cdk6. Further, employing immunoblot analysis we found that treatment of A549 cells with PFE resulted in down-regulation in the expression of biomarkers of cell proliferation i.e. Ki67 and PCNA in a dose-dependent manner. These data establish the involvement of cki-cyclin-cdk network with concomitant decrease in Ki67 and PCNA during the antiproliferative effect of PFE. Taken together, these findings suggest that PFE may have cancer chemopreventive as well as cancer chemotherapeutic effects for lung cancer in humans.
Diffuse and focal palmoplantar keratoderma can be caused by a keratin 6c mutation
Authors: E. Akasaka, H. Nakano, A. Nakano, Y. Toyomaki, N. Takiyoshi, D. Rokunohe, Y. Nishikawa, A. Korekawa, Y. Matsuzaki, Y. Mitsuhashi, D. Sawamura
Journal: British Journal of Dermatology (2011)

The palmoplantar keratodermas (PPKs) are a large group of genodermatoses comprising nearly 60 genetically distinct diseases. They are characterized by hyperkeratosis on the palms and soles with or without extrapalmoplantar hyperkeratotic lesions. Focal PPK is one of the hallmarks of pachyonychia congenita, a rare autosomal dominant disorder resulting from mutations in the keratin genes KRT6A, KRT6B, KRT16 or KRT17. Recently, in-frame deletion mutations of KRT6C have been identified in three families with focal PPK with slight or no nail changes. We report here a novel KRT6C mutation identified in a Japanese family with PPK with phenotypic heterogeneity, presenting with not only focal but also diffuse hyperkeratosis. The proband had diffuse hyperkeratosis on the soles and small focal hyperkeratoses on the palms, while the two other affected individuals showed focal hyperkeratoses on the soles. All three patients were heterozygotes for c.1414G>A in KRT6C, predicted to result in p.Glu472Lys. These findings strongly suggest that screening of patients with nonepidermolytic diffuse PPK, in whom the pathogenic mutations are yet to be determined, might identify mutations in KRT6C.
PITYRIASIS ROSEA—AN AUTOAGGRESSOVE DISEASE?.
Authors: P. R. J. BURCH, N. R. ROWELL
Journal: British Journal of Dermatology (1970)

SUMMARY.— Distributions of the age of onset of pityriasis rosea, in 8 clinical series, are shown to be closely comparable. They fit a simple biological model which also accounts for the sex- and age-distributions of many other age-dependent diseases. The crude sex-ratio (M/F) in the Leeds series (241/246) is close to unity. Month-to-month fluctuations in the presentation of new cases exceed those expected on a random basis: the monthly pattern in the Leeds series broadly resembles those reported previously in an Essex, and a Swedish series. The causes of these temporal fluctuations are unknown. The anatomical distribution of the site of the primary plaque in the Leeds series resembles that found in other series, from other countries. From the statistical evidence, and the clinical course of pityriasis rosea, it is concluded that the aetiology is probably autoaggressive. Autoaggressive diseases are believed to arise in genetically predisposed people as the result of somatic gene mutations in stem cells of the central system that regulates growth. The mutant stem cell propagates a "forbidden clone" of descendant cells, and the products of this clone attack target cells bearing complementary recognition structures or "tissue coding factors". After a latent period, symptoms and signs of the disease appear. The mutant cells of the forbidden-clone evoke an endogenous defence which is probably mediated through immunoglobulins. Extrinsic factors can sometimes disturb the balance between defence and attack to precipitate the onset of autoaggressive disease. The short clinical course of pityriasis rosea, and the rarity of second and third episodes, can probably be attributed to the relatively high efficiency of the endogenous defence mechanism.
Abstract MP13: N-Terminal Pro-Brain Natriuretic Peptide (NT-proBNP) and Risk of Hypertension in the Atherosclerosis Risk in Communities (ARIC) Study
Authors: Julie K Bower, Mariana Lazo, Jonathan Rubin, Kunihiro Matsushita, Ron C Hoogeveen, Christie M Ballantyne, Elizabeth Selvin
Journal: Circulation (2014)

Introduction: Increased plasma N-terminal pro-brain natriuretic peptide (NT-proBNP) concentration reflects cardiac overload and is used to diagnose and stage congestive heart failure. While NT-proBNP exhibits beneficial effects on the cardiovascular system via vasodilatation and diuresis, increased amounts of circulating NT-proBNP also promote release of norepinephrine, which is known to affect blood pressure. Cross-sectional studies report positive associations of NT-pro-BNP with blood pressure, but the prospective association of increased NT-proBNP with risk of hypertension is not well characterized. Thus, the study aim was to evaluate the association of NT-proBNP with incident hypertension in the community-based ARIC Study. Methods: We conducted a prospective cohort analysis of 3,849 participants aged 53-75 years with measured NT-proBNP at ARIC Visit 4 (1996-98) and normal blood pressure; participants with a history of hypertension (medication use or measured BP ≥140/90 mmHg), cardiovascular disease, or heart failure at baseline were excluded. Incident hypertension was defined based on diagnosis or antihypertensive medication use reported during annual follow-up. Results: During a median follow-up of 9 years, there were 2,157 new hypertension cases. There was a significant independent positive association between NT-proBNP levels and risk of hypertension. A 10% increased risk of hypertension was observed per log-unit increase in NT-proBNP (Table). This association was similar among individuals who were normotensive and pre-hypertensive at baseline [HR (95 % CI): 1.08 (1.01-1.15) and 1.11 (1.00-1.24), respectively]. Participants in the highest quartile for NT-proBNP were at 26% increased risk compared to those in the lowest quartile. Conclusions: NT-proBNP is positively associated with incident hypertension in this community-based cohort of middle-aged and older adults. In spite of the vasodilatory effects of increased NT-proBNP, elevated levels predict the development of hypertension.