tbuckley/cabot-search huggingface dataset:
Reflectance mode confocal microscopy and digital image analysis in naevus of Hori and pathogenetic evaluation
Authors: G. Pistone, S. Doukaki, D. Rizzo, M. Aricò, M.R. Bongiorno
Journal: British Journal of Dermatology (2012)

Journal Article Reflectance mode confocal microscopy and digital image analysis in naevus of Hori and pathogenetic evaluation Get access G. Pistone, G. Pistone 1Department of Dermatology, University of Palermo, Via del Vespro 131, 90123 Palermo, Italy Search for other works by this author on: Oxford Academic Google Scholar S. Doukaki, S. Doukaki 1Department of Dermatology, University of Palermo, Via del Vespro 131, 90123 Palermo, Italy Search for other works by this author on: Oxford Academic Google Scholar D. Rizzo, D. Rizzo 1Department of Dermatology, University of Palermo, Via del Vespro 131, 90123 Palermo, Italy Search for other works by this author on: Oxford Academic Google Scholar M. Aricò, M. Aricò 1Department of Dermatology, University of Palermo, Via del Vespro 131, 90123 Palermo, Italy Search for other works by this author on: Oxford Academic Google Scholar M.R. Bongiorno M.R. Bongiorno 1Department of Dermatology, University of Palermo, Via del Vespro 131, 90123 Palermo, Italy Maria Rita Bongiorno. E‐mail: mariarita.bongiorno@unipa.it Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 167, Issue 3, 1 September 2012, Pages 692–694, https://doi.org/10.1111/j.1365-2133.2012.10942.x Published: 01 September 2012
Abstract 4215: SPAG5 upregulation predicts poor prognosis of cervical cancer patients and alters the sensitivity of Taxol treatment via mTOR signaling pathway
Authors: Lin-Jing Yuan, Lan Zhang, Yun Zhou, Min Zheng
Journal: Cancer Research (2014)

Abstract Our previous work found SPAG5 to be upregulated in pelvic lymphnode metastasis positive cervical cancer, the aim of the present study was to examine the role of SPAG5 in proliferation and tumorigenicity of cervical cancers, and its clinical significance in tumor progression. By detecting SPAG5 expression in cervical cancer patients and exploring cervical cancer cell function with SPAG5 downregulation in vitro, our results revealed that SPAG5 upregulated in tumor tissue compared with paired adjacent noncancerous tissues, and the upregulation of SPAG5 in tumor tissues also indicated poor disease free survival which was also an independent prognostic indicator of cervical cancer patients. In vitro study demonstrated that SPAG5 downregulation inhibited cell proliferation and growth significantly by G2/M arrest and apoptosis induction, and also hindered cell migration and invasion. Further study showed different sensitivity of cervical cells to different doses of Taxol treatment under SPAG5 downregulation, which is correlated with mTOR signaling pathway activity. In this study, we demonstrated that SPAG5 upregulation related to poor prognosis of cervical cancer patients, and SPAG5 was a regulator of mTOR activity during Taxol treatment in cervical cancer. Note: This abstract was not presented at the meeting. Citation Format: Lin-Jing Yuan, Lan Zhang, Yun Zhou, Min Zheng. SPAG5 upregulation predicts poor prognosis of cervical cancer patients and alters the sensitivity of Taxol treatment via mTOR signaling pathway. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4215. doi:10.1158/1538-7445.AM2014-4215
Clonal analysis of myelodysplastic syndrome: monosomy 7 is expressed in the myeloid lineage, but not in the lymphoid lineage as detected by fluorescent in situ hybridization
Authors: WR Gerritsen, J Donohue, J Bauman, SC Jhanwar, NA Kernan, H Castro-Malaspina, RJ O'Reilly, JH Bourhis
Journal: Blood (1992)
Enzyme architecture affects DNA topoisomerase I activity and sensitivity to camptothecin.
Authors: Marie Van der Merwe, William Colley, Mary-Ann Bjornsti
Journal: Cancer Research (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

5527 

DNA topoisomerase 1 (Top1) catalyzes the relaxation of supercoiled DNA during DNA replication and transcription. Top1 is also the cellular target of camptothecin (CPT), FDA approved analogs of which are effective new agents in the treatment of human cancers. A conserved central domain of Top1 forms a protein clamp, which is tethered to the active site tyrosine domain by an extended pair of alpha helices (linker domain). This Tyr cleaves one DNA strand to form a covalent enzyme-DNA complex. CPT binding stabilizes the covalent complex, while the collision of replication forks with CPT-Top1-DNA adducts produces DNA lesions that induce cell death. Yeast and human Top1 (yTop1 and hTop1, respectively) exhibit significant sequence and structural similarities, yet CPT toxicity is enhanced in yeast cells expressing hTop versus yTop1. Moreover, in structures of a 70kDa C-terminal fragment of hTop1 covalently linked to DNA, binding of CPT coincides with restricted linker domain movement. These results led us to consider the effects that enzyme architecture and active site mutations have on linker flexibility and CPT sensitivity. A PCR-based method generated y/h Top1 chimeras bearing reciprocal swaps of the non-conserved N-terminal and linker domains. Our findings indicate that in vivo differences in CPT sensitivity are unlikely due to altered protein-protein interactions, dictated by the N-terminus. Rather, introducing a longer yeast linker into hTop1 induced yeast cell lethality. However, when the yeast N-terminus was combined with the yeast linker in hTop1, cell viability and CPT sensitivity were restored. Thus, the functional interaction of these domains modulates enzyme activity and CPT sensitivity. Yet, independent of composition, linker flexibility is also a major determinant of CPT sensitivity. A conserved Gly (721 in yeast and 717 in human) lies in a loop that connects the conserved active site Tyr domain with the flexible linker domain. Mutations of this residue induce a spectrum of CPT sensitivities, without affecting enzyme activity. In yTop1, mutating Gly721 to polar residues (Gln or Asn) increased enzyme sensitivity to CPT, while acidic residues (Asp or Glu) further enhanced this effect. Moreover, these effects were also observed in vivo. In contrast, introducing Gln or Glu at the adjacent position (Leu720) induced CPT resistance. Studies of Leu716 and Gly717 mutations indicate similar effects on hTop1 function. These results suggest alterations in linker structure and flexibility affect Top1 sensitivity to CPT and support a model whereby the conserved Gly provides the flexible hinge that enables linker movement, while the geometry and hydrophobicity of the preceding Leu constrain linker movement to facilitate CPT binding. Supported by ALSAC and NIH CA58755
A memorable patient: A dead man brought to life
Authors: K. Samanta
Journal: BMJ (2002)

On my recent visit to Kolkata, formerly known as Calcutta, I went to a concert. During the interval, a woman approached me in a rather excited state and asked, “Are you Kamal Samanta?” I answered “yes,” and she again asked as if for further confirmation, “Are you Doctor Kamal Samanta, who had gone to live in England?” Again I answered in the affirmative. I realised that my physical appearance has changed in 30 years but not enough to be totally unrecognisable to someone even from the distant past.

“Do you remember me?” she asked …
Effects of Sleep Inertia on Cognition
Authors: Adam T Wertz, Joseph M Ronda, Charles A Czeisler, Kenneth P Wright
Journal: JAMA (2006)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 18120: The Relationship Between Estimated Total Ischemic Time and Hospital Survival in Patients Suffering From Out of Hospital Cardiac Arrest
Authors: Ryan Gunter, Rosa Banuelos, Pratik B Doshi
Journal: Circulation (2014)

Introduction: The incidence of out of hospital cardiac arrest (OHCA) in the U.S. is approximately 400,000 patients per year. Despite quality improvement efforts, overall survival after cardiac arrest remains poor, suggesting that there are other factors at play. One such variable is total ischemic time. This analysis was performed to determine the role of total ischemic time in hospital survival after OHCA. Methods: The OHCA records were queried from the cardiac arrest registry maintained by Houston Fire department from 2007-2012.Association between outcome and predictor variables was assessed using logistic regression. The outcome variable is defined as survival and discharge from the hospital. Our predictor variable of interest was Estimated Total Ischemic Time, which was calculated as the difference between time of Return of Spontaneous Circulation (ROSC) and Fire Rescue Dispatch time. The Hosmer-Lemeshow Goodness of Fit test grouped by ten showed that the null (the model) should not be rejected (p = 0.8678). The final model includes Estimated Total Ischemic Time, age divided into categories by decade, presence of a shockable rhythm, and presence of a witness to the cardiac arrest. Results: The database consisted of 9,074 OHCA cases, of which 446 were included in the final analysis. According to our model, the data suggests there is a decrease in probability of survival as estimated total ischemic time increases. The odds of survival and discharge from the hospital of an OHCA patient with an estimated total ischemic time of 2 minutes is 0.956 (95%CI 0.936 - 0.977) times the odds of a patient whose estimated total ischemic time was 1 minute. Conclusion: Estimated Total Ischemic Time is a significant contributor to the probability of survival, however it is not the only one. This model exemplifies just one example of the importance of adjusting for both prehospital and hospital care in clinical decision-making and healthcare quality improvement.
Do clinicians consider the results of the BinaxNOW Streptococcus pneumoniae urinary antigen test when adapting antibiotic regimens for pneumonia patients?
Authors: M. Matta, S. Kernéis, N. Day, M. Lescat, A. Buu Hoi, E. Varon, L. Gutmann, J.-L. Mainardi
Journal: Clinical Microbiology and Infection (2010)

<h2>Abstract</h2> The BinaxNOW <i>Streptococcus pneumoniae</i> urinary antigen test is a rapid and reliable immunochromatographic test (ICT) for the identification of a pneumococcal aetiology of pneumonia. The aim of this study was to evaluate the attitude of clinicians in their everyday practice towards prescription of the ICT and the impact of its results on the adaptation of the antibiotic therapy when pneumonia is suspected. From October 2007 to March 2008, we prospectively evaluated 541 consecutive inpatients for whom the ICT was performed in our institution. Of the 541 patients evaluated, only 233 (43%) were suspected by the treating physicians to have a pneumonia, 58 of whom had a positive ICT result. Among these 58 patients, four (7%) and 26 (45%), respectively, were treated with amoxycillin monotherapy before and after the ICT result had been obtained (p <10<sup>−4</sup>). Although a positive ICT result led to a rise in the proportion of patients treated with amoxycillin alone, a large number continued to be treated with broader-spectrum antibiotics. These results suggest that prescription monitoring of the ICT should be implemented along with encouragement to adhere more strictly to treatment guidelines.
Abstract 9900: Air Pollution and Stroke: A Systematic Review and Meta-Analysis
Authors: Kuan Ken Lee, Anoop S Shah, David A McAllister, Amanda Hunter, Harish Nair, William Whiteley, Jeremy P Langrish, David E Newby, Nicholas L Mills
Journal: Circulation (2014)

Introduction: Stroke is responsible for 5 million deaths per annum with two-thirds occurring in low- and middle-income countries. Whilst exposure to outdoor air pollution is strongly associated with acute cardiac events, the relationship between air pollution and stroke is less clear. We systematically reviewed and meta-analysed the evidence examining the association between air pollution and stroke hospitalisation or mortality. Methods: Five databases were searched for studies investigating the association between daily increases in gaseous pollutants (carbon monoxide, sulphur dioxide, nitrogen dioxide, ozone) and particulate matter (diameter <2·5 or 10 μm: PM2·5 and PM10), and stroke hospitalisation or mortality. We used a random-effects model to derive overall risk estimates per pollutant. Results: We identified 2,747 articles of which 238 were reviewed in-depth with 104 satisfying our inclusion criteria. This provided a total of 6.2 million events across 28 countries. Stroke hospitalisation or death f...
The Cross-Cover Resident
Authors: Megan A. Moreno
Journal: JAMA (2003)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 2608: Dissecting Myc inhibition as a cancer therapy
Authors: Nicholas J. H. Salisbury, Catherine H. Wilson, Dan Lu, Trevor D. Littlewood, Gerard I. Evan
Journal: Cancer Research (2014)

Abstract The MYC family of basic helix-loop-helix leucine zipper (bHLH-LZ) transcription factors are critical for cell growth, proliferation and apoptosis. c-Myc is deregulated in most, if not all, cancers making it an attractive target for cancer therapy. Previously we have shown that Myc inhibition by a mutant c-Myc derivative, Omomyc, leads to tumor regression in several cancer models, notably in murine KRasG12D-driven lung adenocarcinomas. The mechanism by which Omomyc expression leads to tumor regression has not been fully elucidated, nor do we know the biochemical properties of a small molecule that could replicate Omomyc's activity. Therefore, we designed two novel Omomyc mutants (ΔMyc and MMO) that are hypothesised not to interact with the full Omomyc interactome but are still able to inhibit Myc transactivation. We subsequently created human lung adenocarcinoma cell lines (A549s) and transgenic mice which inducibly express Omomyc, ΔMyc and MMO. Unexpectedly Omomyc and ΔMyc inhibited proliferation in vitro but not MMO. In vivo, expression of the mutants has been detected and we are currently assessing their effect on KRasG12D-driven adenocarcinomas. Citation Format: Nicholas J. H. Salisbury, Catherine H. Wilson, Dan Lu, Trevor D. Littlewood, Gerard I. Evan. Dissecting Myc inhibition as a cancer therapy. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2608. doi:10.1158/1538-7445.AM2014-2608
Abstract MP48: Social Network, Social Support, and the Risk of Incident Stroke: The Atherosclerosis Risk in Communities Study
Authors: Mako Nagayoshi, Susan A Everson-Rose, Hiroyasu Iso, Thomas H Mosley, Kathryn M Rose, Pamela L Lutsey
Journal: Circulation (2014)

Background and Purpose: Having a small social network and lack of social support have been associated with incident coronary heart disease, but little is known about their association with incident stroke. Thus, we assessed the association of a small social network and lack of social support with risk of incident stroke and evaluated whether the relation was mediated by vital exhaustion and inflammation. Methods: The Atherosclerosis Risk in Communities (ARIC) Study measured social network and social support in 13,686 men and women (mean, 57 ± 5.7 years, 56% female, 24% black; 76% white) initially free of stroke. The 10-item Lubben Social Network Scale and 16-item Interpersonal Support Evaluation List-Short Form were used to assess social network size and social support, respectively. Results: Over a median follow-up of 18.6-years, 905 incident strokes occurred. Relative to participants with a large social network, those with a small social network had a higher risk of stroke [HR (95% CI): 1.43 (1.03-2.00)] after adjustment for demographic and socioeconomic characteristics and marital status ( Table ). Further adjustment for other potential confounders attenuated the association slightly. Vital exhaustion, but not inflammation, partly mediated the association between a small social network and stroke. Social support was unrelated to incident stroke. Conclusions: In this sample of US community-dwelling men and women, having a small social network was associated with excess risk of incident stroke. As with other cardiovascular conditions, having a small social network appears to be associated with modestly increased risk of incident stroke.
Evaluation of c-FLIP(long), c-FLIP(short), and caspase 8 expression in primary tumors using a semiquantitative multiplex LSC-based assay
Authors: Jason A. Wall, Christopher Thor, Susan Cottrell, Hugh Rand, Daniel Branstetter, Yang Pan
Journal: Clinical Cancer Research (2010)

Abstract Recent studies have demonstrated the value in quantification of tumor expression and mutational status of growth factor signaling components as potential predictors of clinical response. Quantification of elements in the apoptosis pathways may provide a similar value in predicting clinical response to new therapies based on induction of apoptosis through death-receptor-mediated signaling. To that end, we have qualified a 5-color multiplexed immune histofluorescent tissue-based assay to provide semiquantitative measurement of expression of death-inducing signaling complex (DISC) components. The DISC components we measured in FFPE archival samples from human solid tumors are caspase 8, c-FLIP(long) and c-FLIP(short). Additional components of the multiplex were a pan-keratin reagent for identification of cytokeratin expressing epithelial tumor cells, and a histone H3 reagent for staining quality control. Image collection was accomplished via laser scanning cytometry (LSC) with a data pipeline which generates FCS3.0 (flow cytometry standard 3.0) files. This allowed intensity analysis of gated tumor enriched regions via standard flow cytometry analysis software. Antibodies were identified via in vitro screens using high/low target expressing cell lines, and further tested using xenografts of the cell lines of interest. The assay was qualified by examining day-to-day, slide-to-slide, and inter-operator variability using xenograft TMA (tissue micro-array) controls. The intensity of stained samples over 24 hours was stable. Total experimental %CV was demonstrated to be less than 25%. We analyzed 6 commercially available cancer TMA slides containing 387 tumor cores representing colon, lung, pancreas, breast, sarcoma, and non-Hodgkin's lymphoma, along with 43 corresponding normal cores. We found a wide range of expression of caspase 8, c-FLIP(l), and c-FLIP(s) within each tumor type, as well as clear differences between normal and tumor tissues. These findings indicate a potential for stratification of clinical response by DISC protein expression in therapeutics that act via death receptor mediated apoptosis.
Abstract 200: Computer-Assisted Monitoring of Hemodynamic Condition During a Simulated Experiment of Hypovolemia Using Lower Body Negative Pressure
Authors: Ashwin Belle, Maxwell Spadafore, Harm Derksen, Kevin Ward, Kayvan Najarian
Journal: Circulation (2014)

Background:  Real-time physiologic status monitoring of hemorrhaging war-fighter or civilian from the point of injury and along ensuing echelons of care in austere environments pose significant challenges. These challenges range for those arising from the environment to the physical profiles of monitors and the types of measures they make.   Objective:  To develop an automated clinical support system capable of using low level physiologic signals such as the electrocardiogram (ECG) to monitor hemodynamic compensation in response to injury or illness.   Methods:  An adapted low profile portable and wearable armband monitor was used to collect prolonged measurement of a single lead ECG from volunteer human subjects undergoing lower body negative pressure (LBNP) as a model of human hemorrhage. Fifty-five subjects underwent 5-minute intervals of progressive LBNP until hemodynamic decompensation at which time LBNP was halted. Using several advanced signal processing techniques including novel applications of the Stockwell Transform and L-1 Norm optimized taut-string theory, hidden underlying bio-markers were extracted from the ECG signal which was then used to develop a predictive model using support vector regression using radial basis kernel to predict the severity of blood loss with respect to LBNP levels.   Results:  Using by-subject cross validation for each of the 55 subjects, the Pearson’s correlation coefficient was calculated between the algorithm’s estimates and the actual LBNP levels for each subject. High correlation between the prediction and actual levels were observed with a mean correlation coefficient across all subjects of 0.862 and σ of 0.072. The mean r2 value was 0.928 with σ of 0.04. The system predicted changes in stability prior to any significant changes in heart rate and blood pressure among subjects during LBNP.   Conclusions:  The developed ECG analytic technique has provided encouraging results and has shown potential to be applicable in a variety of critical care environments and applications where detection of hemodynamic changes prior to overt decompensation would allow early intervention. This when used with wearable sensors opens up new possibilities for monitoring in different locations.