tbuckley/cabot-search huggingface dataset:
Proteomics of red blood cell from patients with obstructive sleep apnea
Authors: Amélia Feliciano, Vesna Bozanic, Vukosava Milic Torres, Rune Matthiesen, Ana Carvalho, Andreia Almeida, Bruno Alexandre, Fátima Vaz, Atul Malhotra, Paula Pinto, Cristina Bárbara, Deborah Penque
Journal: European Respiratory Journal (2014)

Obstructive sleep apnea (OSA) is responsible for metabolic/cardiovascular consequences, but its molecular mechanisms are poorly defined. Using 2-D Fluorescence Difference Gel Electrophoresis and mass spectrometry proteomics evaluation of red blood cells (RBC), we plan to identify dysregulated proteins (possible OSA biomarkers). To assess proteome variations, RBC were collected from blood of moderate/severe OSA (n=12) or simple snoring (n=12) at pre- and post- night sleep. RBC samples were randomized pooled to 3 replicates/group of patients/condition. Samples were fractionated into membrane and soluble fractions, being the latter depleted of hemoglobin. Soluble RBC fractions were labeled with GE fluorescente dyes and separated by 2DE. After that the images were acquired in an Imager and analysed by Progenesis SameSpots software. 70 spots were differentially expressed (p‹0.05) between OSA and simple snoring and at pre/post-night sleep were excised from a Coomassie-stained preparative gels and proteins identified by MALDI-TOF/TOF MS. Pathway analysis was carried out by Ingenuity Pathway Analysis. The most significant networks were associated with cellular response to hydrogen peroxide/reactive oxygen species. The complete identification/validation of those proteins will provide better understanding of OSA and translated into diagnosis/prognosis tools. Work supported by Harvard Medical School-Portugal Program (HMSP-ICJ/0022/2011), FCT/Poly-Annual Funding Program and FEDER/Saude XXI Program. 2-DIGE images were captured at Instituto de Tecnologia Quimica/Biologica, Portugal. Work approved by the Ethical Committees of involved institutions and Comissao Nacional de Protecao de Dados.
Lack of glucocorticoid-induced leucine zipper (GILZ) deregulates B-cell survival and results in B-cell lymphocytosis in mice
Authors: Stefano Bruscoli, Michele Biagioli, Daniele Sorcini, Tiziana Frammartino, Monica Cimino, Paolo Sportoletti, Emanuela Mazzon, Oxana Bereshchenko, Carlo Riccardi
Journal: Blood (2015)
Differences and Commonalities of National Field Epidemiology Training Programmes in Europe
Authors: G Krause, P Aavitsland, K Alpers, A Barrasa, V Bremer, B Helynck, A Perra
Journal: Eurosurveillance (2009)

From 1994 to 2009, national field epidemiology training programmes (FETP) have been installed in Spain, Germany, Italy, France and Norway. During their two year duration, different components of the FETP are devised as follows: 63-79 weeks are spent on projects in hosting institutes, 2-26 weeks in outside projects, 9-30 weeks in courses and modules, and 1-2 weeks in scientific conferences. A considerable proportion of the Spanish FETP has is provided conventional class room training . The content of the modules is very similar for all programmes. Except from the Italian programme, all focus on infectious disease epidemiology. The German and Norwegian programmes are so called EPIET-associated programmesas their participants are integrated in the modules and the supervision offered by EPIET, but salaries, facilitators, and training sites are provided by the national programme. These EPIET-associated programmes require strong communications skills in English. Alumni of all five FETP are generally working within the public health work force in their respective countries or at international level, many of them in leading functions. Although three new FETP have been installed since the last published Euroroundup in Eurosurveillance on European FETP in 2001, the progress with respect to the establishment of national FETP or EPIET-associated programmes has been slow. Member States should be aware of how much support EPIET can offer for the establishment of national FETP or EPIET-associated programmes. However, they also need to be ready to provide the necessary resources, the administrative environment and long-term dedication to make field epidemiology training work.
Phase II trial of fludarabine phosphate in lymphoma: an effective new agent in low-grade lymphoma.
Authors: J R Redman, F Cabanillas, W S Velasquez, P McLaughlin, F B Hagemeister, F Swan, M A Rodriguez, W K Plunkett, M J Keating
Journal: Journal of Clinical Oncology (1992)

PURPOSE In a phase II trial we investigated fludarabine phosphate (FAMP) as therapy for patients with relapsed lymphoma to determine its effectiveness and toxicity in this disease. PATIENTS AND METHODS The 67 assessable patients had a median age of 56 years and had received a median of three chemotherapy regimens before treatment with FAMP. The starting dose was 25 mg/m2 administered intravenously over 30 minutes daily for 5 days every 3 to 4 weeks. RESULTS High response rates were observed for follicular small cleaved-cell lymphoma (FSCCL) (62%), follicular mixed small- and large-cell lymphoma (80%), and follicular large-cell lymphoma (FLCL) (100%). Responses also occurred in small lymphocytic lymphoma (SLL) (33%), transformed lymphoma (33%), mycosis fungoides (40%), and Hodgkin's disease (25%). No responses were observed in other intermediate- or high-grade lymphomas (N = 20). Overall, there were five patients with a complete response, 23 patients with a partial response, and an overall response rate of 37%. Toxicity was primarily hematologic and infectious. No significant gastrointestinal, hepatic, renal, or neurologic toxicity occurred. CONCLUSIONS We conclude that FAMP has major activity in follicular lymphoma. Fundamental research is needed to understand this differential efficacy in low-grade lymphoma yet lack of efficacy in intermediate- and high-grade lymphoma. Clinical investigations should be done using FAMP in varying dose schedules and in combination regimens.
Regulation of VEGF receptors and co-factors by hypoxia and hyperoxia
Authors: Thomas Blythe, Shaney Barratt, Caroline Jarrett, Gavin Welsh, Ann Millar
Journal: European Respiratory Journal (2012)

VEGF exerts its biological effect through specific receptors, VEGFR-1 and VEGFR-2 and co-receptors, neuropilin-1 and neuropilin-2, keading to a complex regulatory sytem. The presence of hypoxia in lung disease and its treatment by high flow oxygen have been proposed to contribute to lung injury. We hypothesised that hypoxia and hyperoxia may lead to changes receptor and co-receptor expression and hence regulation of VEGF bioactivity.  A549 (ATCC) as a model for lung epithelial cells and Human pulmonary microvascular endothelial cells (HMVEC-l) were obtained and cultured in for 24 hours in a PROOX chamber in normoxic (N), hyperoxic 90% O2 (H), hypoxic conditions were represented by culture in the presence of Cobalt chloride (CoCl 2 ). The cells were then lysed for protein or RNA extraction. Expression of VEGFR1, 2 and NRP-1, NRP-2 was established at both mRNA and protein level by q-PCR, immunofluorescence and Western blotting.  No significant changes were detected in A549 expression of VEGF-R1, NRP-2, however NRP1 was significantly reduced compared to control values (p  These results suggest that changes in oxygen tension may have a significant effect on VEGF bioactivity with potential implications for the use of oxygen therapy.
Rethinking Preoperative Breast Magnetic Resonance Imaging
Authors: Habib Rahbar, Constance D. Lehman
Journal: JAMA Oncology (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Oncology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Oncology journal
Etiology and evolution of bronchiectasis in men
Authors: Hamida Kwas, Imen Elmjendel, Ines Zendah, Aymen Ayari, Habib Ghedira
Journal: European Respiratory Journal (2013)

Introduction:  Although considered as an orphan disease in the developed countries, bronchiectasis are frequent in our country as in all emerging ones. They are common in women but also observed in man.   Aim:  To determine the etiology and prognosis of the bronchiectasis in men.   Patients and methods:  Retrospective study including 32 men with bronchiectasis followed in our department during the period between 2003 and 2012.   Results:  The mean age was 52 years. The diagnosis of bronchiectasis was confirmed in all patients. Bronchiectasis were post-tuberculosis in 12 cases. The other etiologies are represented by: Severe respiratory infection in childhood (8 cases), primary ciliary dyskinesia (2 cases), the inhalation of a foreign body (1 case) and the gastroesophageal reflux (1 case). Bronchiectasis are idiopathic in 8 cases. Chest CT scan showed diffused bronchiectasis in 18 cases, localized in 14 cases, and bilateral in 19 cases. Complications are dominated by broncho-pulmonary infections (15 cases) and hemoptysis (13 cases). Ten patients with diffused and bilateral bronchiectasis have evolved to chronic respiratory failure.   Conclusion:  Bronchiectasis is frequent in men. Etiologies remain dominated, in our context by tuberculosis despite the national program of tuberculosis control.
Abstract 12848: Arterial Coronary Artery Bypass Grafting is Associated With Improved Long-Term Survival in Elderly Patients
Authors: Rephael Mohr, Benjamin Medalion, Zvi Raviv, Amir Kremer, Nahum Nesher, Yanai Ben Gal, Dmitry Pevni
Journal: Circulation (2014)

Introduction: Bilateral Internal Thoracic Artery (ITA) grafting in the elderly is controversial because of their shorter life expectancy , and the excellent survival benefit obtained with single ITA. Methods: One thousand and fourty five patients 70 years old or older who underwent BITA grafting between 1996 and 2008 were compared with 582 patients who underwent CABG with single ITA and SVG and 249 patients who underwent CABG with single ITA and RA. Hypothesis: The purpose of this study is to compare outcome of bilateral ITA(BITA) grafting to that of single ITA and other conduits, such as saphenous veins(SVG) or radial artery(RA), in the subset of elderly patients. In order to account for differences between groups in preoperative characteristics multivariate analyses defined predictors of early and late mortality after forcing propensity score with patients’ characteristics into the models Results: Occurrence of female gender (27% versus 42% and 34% , in the B ITA, RA and SVG groups respectively) diabete...
Effect of Antigen Density on Complement-Mediated Lysis, T-Cell-Mediated Killing, and Antigenic Modulation&lt;xref ref-type="fn" rid="fn2"&gt;&lt;sup&gt;2&lt;/sup&gt;&lt;/xref&gt;
Authors: J Lesley, R Hyman, G Dennert
Journal: JNCI Journal of the National Cancer Institute (1974)

The H-2 content of eight H-2d myeloma lines, two H-2d lymphomas, and the H-2d mastocytoma P815 was tested by quantitative absorption and direct binding of 125I-anti-H-2d. The cell lines were then compared for: 1) sensitivity to anti-H-2d and complement lysis, 2) sensitivity to allogeneic T-cell-mediated cytotoxicity, 3) antigenic modulation when cultured in excess anti-H-2d at 37° C, and 4) removal of anti-H-2 antibody from the cell surface in the presence of excess anti-H-2d. Generally, the cells behaved predictably from their surface antigen content. In complement-mediated cytotoxic assays, the myelomas and lymphomas showed a threshold effect with a given amount of surface antigen, below which complement-mediated killing was poor and above which killing was complete. PSIS, however, was poorly sensitive to complement-mediated lysis, though it had more H-2 antigen per unit area than any other cell line. For all of the lines, sensitivity to cell-mediated cytotoxicity showed a continuous progression: Cells with a high content of antigen tended to be better targets for cell-mediated lysis, whereas those with a lower antigen content tended to be poorer targets; those with an intermediate concentration varied considerably. Cell lines, poorly sensitive to antibody-complement lysis because of a low antigen density, were sensitive to T-cell lysis. Under modulating conditions, all the myelomas lost antibody from their surface to some degree; the extent of reduction of antibody paralleled their loss of sensitivity to complement-mediated Iysis.
Hospital practice explains variation in outcomes in extremely premature infants, US study finds
Authors: M. McCarthy
Journal: BMJ (2015)

Much of the variation seen in outcomes among infants born at 22, 23, or 24 weeks of gestation can be explained by differences in hospitals’ practices on whether to initiate active treatment to save the lives of these extremely premature newborns, concludes a new study in the New England Journal of Medicine .1

The study found that hospitals at which active treatment was more often started had higher rates of risk-adjusted survival, both with and without impairment, than hospitals at which active treatment was less often started.

Currently, active intervention, such as the administration of surfactant therapy, intubation, ventilatory support, resuscitation, and parenteral support, is generally not recommended for infants born before 22 weeks of gestation. But for infants born at or after 22 weeks practice varies from institution to institution, and the American Academy of Pediatrics and the American Congress of Obstetricians and …
The renin-angiotensin system: a potential therapeutic target in ARDS?
Authors: J Elder
Journal: Thorax (2005)

▴ Imai Y, Kuba K, Rao S, et al . Angiotensin-converting enzyme 2 protects from severe acute lung failure. Nature2005;436:112–6

[OpenUrl][1][CrossRef][2][PubMed][3]



This paper reports a critical role of the renin-angiotensin system (RAS) in the pathogenesis of acute lung injury (ALI) in the mouse. Angiotensin converting enzyme (ACE) and its counter-regulatory homologue ACE2 determine the levels of angiotensin II (ATII) within the lung. By a series of logical stepwise experiments, manipulating …

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Multigenerational Undernutrition Increases Susceptibility to Obesity and Diabetes that Is Not Reversed after Dietary Recuperation
Authors: Anandwardhan A Hardikar, Sarang N Satoor, Mahesh S Karandikar, Mugdha V Joglekar, Amrutesh S Puranik, Wilson Wong, Sandeep Kumar, Amita Limaye, Dattatray S Bhat, Andrzej S Januszewski, Malati R Umrani, Amaresh K Ranjan, Kishori Apte, Pranav Yajnik, Ramesh R Bhonde, Sanjeev Galande, Anthony C Keech, Alicia J Jenkins, Chittaranjan S Yajnik
Journal: Cell Metabolism (2015)
Cytokine Production Regulating Th1 and Th2 Cytokines in Hemophagocytic Lymphohistiocytosis
Authors: Yuko Osugi, Junichi Hara, Shinichi Tagawa, Kenji Takai, Gaku Hosoi, Yoshiko Matsuda, Hideaki Ohta, Hiroyuki Fujisaki, Michiko Kobayashi, Naoki Sakata, Keisei Kawa-Ha, Shintaro Okada, Akio Tawa
Journal: Blood (1997)
Arteriovenous fistula (AVF) for the treatement of severe COPD
Authors: Patricio Rioseco, Rodrigo Urrea, Susana Muñoz, Patricia Velazquez
Journal: European Respiratory Journal (2013)

COPD patients have dyspnea limiting exercise capacity for different mechanisms. The gasometric anomaly is due to a V/Q missmatch causing venous admixture which has not been addressed therapeutically so far. Theoretically an AVF could increase venous content of 02 so that blood leaving the left ventricule has a higher than expected Pa02. This, along with the associated increase in cardiac output leads to an increased delivery of 02 particularly to skeletal muscle thus improving its performance.In a patient with severe COPD whom with full therapy fails to improve dyspnea and hypoxemia which limits his exercise capacity we made a peripheral arteriovenous fistula as a therapeutic intent. Spirometry shows an initial FEV1 of 0.74 l/sec, FVC of 1.97 l, hypoxemia of 56.8 mm Hg, Hb 02 saturation of 82.9%. 6MWT with a distance of 300 m (61% of predetermined value) that improved by 108 m providing 02 2 l/min. Echocardiography shows a PASP of 26 mm Hg and a 60% LV ejection fraction. The Saint George9s Respiratory Questionnaire is 73.8%, The CAT is 38 points and BODE Index is 6 points. After 4 weeks of AVF neither spyrometric nor echocardiographic changes were seen but improvement in Pa02 to 68 mm Hg and Sa02 to 93%. The 6MWT increased to 425 m. SGRQ improved 3.88 points, BODE improved to 3 points and CAT to 21 points. We conclude that AVF determines improvement in exercise capacity and better control of disease resulting in better quality of life constituting a non pharmacological aid in advanced COPD patients who fail to improve with full medical therapy. In patient selection is important to evaluate cardiovascular and peripheral vascular status since in theory AVF could lead to RV failure and peripheral blood steal syndrome.
Loss of RACK1 Promotes Metastasis of Gastric Cancer by Inducing a miR-302c/IL8 Signaling Loop
Authors: Ling Chen, Lingqiang Min, Xuefei Wang, Junjie Zhao, Hua Chen, Jing Qin, Weidong Chen, Zhenbin Shen, Zhaoqing Tang, Qiangjun Gan, Yuanyuan Ruan, Yihong Sun, Xinyu Qin, Jianxin Gu
Journal: Cancer Research (2015)

Abstract Gastric cancer remains the third leading cause of cancer-related mortality worldwide, and invasion and metastasis of gastric cancer represent the major reason for its poor prognosis. In this study, we found that loss of the receptor for activated C-kinase 1 (RACK1) promoted the metastasis of gastric cancer by enhancing the autocrine of IL8 in vitro and in vivo. microRNA (miRNA; miR) array identified that RACK1 modulated the expression of a series of miRNAs, including the miR-302 cluster, and RACK1 modulated the IL8 expression and tumor invasion through miRNA-302c. Moreover, upregulation of IL8 in turn decreased the level of miRNA-302c and induced IL8 expression in a feedback manner. Tissue microarray also indicated that RACK1 was correlated with invasion/metastasis phenotype, IL8 expression, as well as 5-year survival in clinical cases of gastric cancer. Together, our results imply that loss of RACK1 in gastric cancer links epigenetics to inflammatory cytokines to promote tumor metastasis. Cancer Res; 75(18); 3832–41. ©2015 AACR.
The dual and opposite role of the TM6SF2‐rs58542926 variant in protecting against cardiovascular disease and conferring risk for nonalcoholic fatty liver: A meta‐analysis
Authors: Carlos J. Pirola, Silvia Sookoian
Journal: Hepatology (2015)

The aim of this work was to estimate the strength of the effect of the TM6SF2 E167K (rs58542926 C/T) variant on blood lipid traits and nonalcoholic fatty liver disease (NAFLD) across different populations. We performed a systematic review by a meta‐analysis; literature searches identified 10 studies. The rs58542926 exerts a significant role in modulating lipid traits, including total cholesterol (TC), low‐density lipoprotein cholesterol (LDL‐C), triglycerides (TG), and NAFLD. However, this influence on lipids and NAFLD is opposite between genotypes in the dominant model of inheritance. Pooled estimates of random effects in 101,326 individuals showed that carriers of the minor T allele (EK+KK individuals), compared with subjects homozygous for the ancestral C allele (EE genotype), are protected from cardiovascular disease (CVD), showing lower levels of TC, LDL‐C, and TG; the differences in mean ± standard error (mg/dL) are −8.38 ± 1.56, −3.7 ± 0.9, and −9.4 ± 2.1, respectively. The rs58542926 variant was not associated with high‐density lipoprotein cholesterol in a large sample (n = 91,937). In contrast, carriers of the T allele showed a moderate effect on the risk of NAFLD (odds ratio: 2.13; 95% confidence interval: 1.36–3.30; P = 0.0009; n = 3273) and approximately ∼2.2% higher lipid fat content when compared with homozygous EE (n = 3,413). Conclusions: The rs58542926 appears to be an important modifier of blood lipid traits in different populations. As a challenge for personalized medicine, the C‐allele, which has an overall frequency as high as 93%, is associated with higher blood lipids, whereas the T allele confers risk for NAFLD; in turn, CVD and NAFLD are strongly related outcomes. Although the variant confers protection against CVD at the expense of an increased risk of NAFLD, it does not explain the link between these two complex diseases.(H epatology 2015;62:1742–1756)
Does Decreasing Variability Affect Quality?
Authors: David M. Mahvi
Journal: JAMA Surgery (2015)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Surgery HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Surgery journal