SOME CHALLENGING ASPECTS OF HEMOGLOBIN METABOLISM
Authors: C J WATSON
Journal: Annals of Internal Medicine (1957)

Article1 October 1957SOME CHALLENGING ASPECTS OF HEMOGLOBIN METABOLISMCECIL J. WATSON, M.D., F.A.C.P.CECIL J. WATSON, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-47-4-611 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptMay I say, Mr. President, that the honor of the John Phillips Lectureship of this College conveys mingled feelings of gratification and humility, much enhanced by contemplating the list of distinguished men who have preceded me on the 20 occasions that this lecture in memory of Dr. Phillips has been given. I sincerely acknowledge my appreciation of this great privilege.The work from our laboratory which I shall have occasion to mention was accomplished only through the generous effort of many associates at one time or another in the last 25 years. I shall strive to represent faithfully their important...Bibliography1. Watson CJ: The pyrrol pigments and hemoglobin catabolism, The 1st E. T. Bell Lecture of the Minnesota Pathological Society; Minnesota Med. 39: 294, 403, and 467, 1956. Google Scholar2. SheminRittenberg DD (a) : The biological utilization of glycine for the synthesis of the protoporphyrin of hemoglobin, J. Biol. Chem. 166: 621, 1946. (b) Shemin, D., and Wittenberg, J.: The mechanism of porphyrin formation. The role of the tricarboxylic acid cycle, J. Biol. Chem. 192: 315, 1951. (c) Shemin, D., Russell, C. S., and Abramsky, T.: The succinate-glycine cycle. I. The mechanism of pyrrole synthesis, J. Biol. Chem. 215: 613, 1955. CrossrefMedlineGoogle Scholar3. SheminRussell DCS: δ-Amino levulinic acid: its role in the biosynthesis of porphyrins, J. Am. Chem. Soc. 75: 4873, 1953. CrossrefGoogle Scholar4. SchmidShemin RD: The enzymatic formation of porphobilinogen from δ-Aminolevulinic acid and its conversion to protoporphyrin, J. Am. Chem. Soc. 77: 506, 1955. CrossrefGoogle Scholar5. Westall RG: Isolation of porphobilinogen from the urine of a patient with acute porphyria, Nature, London 170: 614, 1952. CrossrefMedlineGoogle Scholar6. CooksonRimington GHC (a) : Porphobilinogen: chemical constitution, Nature, London 171: 875, 1953. (b) Cookson, G. H., and Rimington, C.: Porphobilinogen, Biochem. J. 57: 476, 1954. CrossrefMedlineGoogle Scholar7. Rimington C: The biosynthesis of haemoglobin, Brit. M. J. 2: 189, 1956. CrossrefMedlineGoogle Scholar8. NeubergerScott AJJ: Aminolaevulic acid and porphyrin biosynthesis, Nature, London 172: 1093, 1953. CrossrefMedlineGoogle Scholar9. WatsonDeMelloSchwartzHawkinsonBossenmaier CJRPSVI: Porphyrin chromogens or precursors in urine, blood, bile and feces, J. Lab. and Clin. Med. 37: 831, 1951. MedlineGoogle Scholar10. NeveLabbeAldrich RLR: Reduced uroporphyrin III in the biosynthesis of heme, J. Am. Chem. Soc. 78: 691, 1956. CrossrefGoogle Scholar11. Bashour FA (a) : The conversion of reduced uroporphyrin I to coproporphyrin and protoporphyrin by dog liver homogenate, J. Clin. Investigation 35: 689, 1956. (b) Bashour, F. A.: Study of uroporphyrin metabolism with special reference to conditions other than porphyria, Ph. D. Thesis, University of Minnesota, Minneapolis, 1957. Google Scholar12. Watson CJ: Porphyrin metabolism in the anemias, The George R. Minot Lecture, Arch. Int. Med. 99: 323, 1957. CrossrefGoogle Scholar13. GrinsteinWikoffPimenta de MelloWatson MHMRCJ: Isotopic studies of porphyrin and hemoglobin metabolism. II. The biosynthesis of coproporphyrin III in experimental lead poisoning, J. Biol. Chem. 182: 723, 1950. CrossrefGoogle Scholar14. GrinsteinAldrichHawkinsonWatson MRAVCJ (a) : An isotopic study of porphyrin and hemoglobin metabolism in a case of porphyria, J. Biol. Chem. 179: 983, 1949. (b) Grinstein, M., Aldrich, R. S., Hawkinson, V., Lowry, P., and Watson, C. J.: Photosensitive or congenital porphyria with hemolytic anemia. II. Isotopic studies of porphyrin and hemoglobin metabolism, Blood 6: 699, 1951. CrossrefMedlineGoogle Scholar15. GrayNeubergerSneath CHAPH: Studies in congenital porphyria. II. Incorporation of 15 N in the stercobilin in the normal and in the porphyric, Biochem. J. 47: 87, 1950. CrossrefMedlineGoogle Scholar16. LondonWestSheminRittenberg IMRDD: Porphyrin formation and hemoglobin metabolism in congenital porphyria, J. Biol. Chem., 184: 1, 1950. CrossrefMedlineGoogle Scholar17. LowryHawkinsonWatson PHVECJ: An isotopic study of type III porphyrins and hemoglobin metabolism in an unusual case of "mixed" porphyria, Metabolism 1: 149, 1952. MedlineGoogle Scholar18. LondonWestSheminRittenberg IMRDD: On the origin of bile pigment in normal man, J. Biol. Chem. 184: 351, 1950. CrossrefMedlineGoogle Scholar19. Watson CJ (a) : Über Stercobilin und Porphyrine aus Kot, Ztschr. f. physiol. chem. 204: 57, 1932. (b) Watson, C. J.: Über krystallisiertes Stercobilin bzw. Urobilin, Ztschr. f. physiol. Chem. 233: 39, 1935. (c) Watson, C. J., Lowry, P. T., Sborov, V. E., Hollinshead, W. N., Kohan, S., and Orrego Matte, H.: A simple method of isolation of crystalline stercobilin or urobilin from feces, J. Biol. Chem. 200: 697, 1953. CrossrefGoogle Scholar20. Watson CJ: The direct preparation of crystalline urobilin from bilirubin, J. Biol. Chem. 200: 691, 1953. CrossrefMedlineGoogle Scholar21. SchwartzWatson SCJ (a) : Isolation of a dextrorotatory urobilin from human fistula bile, Proc. Soc. Exper. Biol. and Med. 49: 643, 1942. (b) Sborov, V., Jay, A. R., and Watson, C. J.: The effect of Aureomycin on urobilinogen formation and the fecal flora, J. Lab. and Clin. Med. 37: 52, 1951. (c) Lowry, P. T., and Watson, C. J.: The isolation of crystalline d-urobilinogen, J. Biol. Chem. 218: 641, 1956. (d) Watson, C. J., and Lowry, P. T.: A further study of crystalline d-urobilin, J. Biol. Chem. 218: 633, 1956. (e) Watson, C. J., Graham, S., Ziegler, N., and Lowry, P. T.: The intestinal formation and interrelationship of members of the urobilinogen group with special reference to the dextrorotatory form, Tr. A. Am. Physicians 67: 242, 1954. CrossrefGoogle Scholar22. FischerNiemann HG: Zur Kenntnis des Gallenfarbstoffes. VIII. Mitteil. Mesobiliviolin, Mesobiliviolinogen und die Kondensation von Mesobilirubinogen mit Aldehyden unter Bildung von neuen Spaltprodukten. Diazofarbstoff des Mesobilirubins, Ztschr. f. physiol. Chem. 137: 292, 1924. CrossrefGoogle Scholar23. LembergLockwoodWyndham RWHRA: Urobilins and urobilinogens, Australian J. Exper. Biol. and M. Sc. 16: 169, 1938. CrossrefGoogle Scholar24. Baumgärtel T: Physiologie und Pathologie des Bilirubinstoffwechsels als Grundlagen der Ikterusforschung, 1950, Georg Thieme Verlag, Stuttgart. Google Scholar25. Stich W: Klinische Untersuchungen zur Unterscheidung von Sterkobilin und Urobilin mit der Pentdyopentreaktion, Klin. Wchnschr. 26: 365, 1948. CrossrefMedlineGoogle Scholar26. Rudolph H: Chemie und Klinik der Bilirubinreduktions-produkte Urobilin und Sterkobilin, 1952, Georg Thieme, Leipzig. Google Scholar27. MaierSchwarz CKJ: Über eine neue Differenzierungsmöglichkeit der Urobilinkörper in Urin, Praxis 42: 156, 1953. MedlineGoogle Scholar28. Watson CJ: The pyrrol pigments with particular reference to normal and pathologic hemoglobin metabolism, in Downey's Handbook of hematology, vol. IV, 1938, P. Hoeber, New York, p. 2447. Google Scholar29. BingoldStich KW: Fortschritte auf dem Gebiet des Blutfarbstoffes, Ergebn. d. inn. Med. u. Kinderheilk. 5: 707, 1954. MedlineGoogle Scholar30. LowryBossenmaierWatson PTICJ (a) : A method for the isolation of bilirubin from feces, J. Biol. Chem. 202: 305, 1953. (b) Lowry, P. T., Ziegler, N. R., Cardinal, R., and Watson, C. J.: The conversion of N15 labeled mesobilirubinogen to stercobilinogen by fecal bacteria, J. Biol. Chem. 208: 543, 1954. CrossrefMedlineGoogle Scholar31. Müller F (a) : Untersuchungen über Ikterus, Ztschr. f. klin. Med. 12: 45, 1887. (b) Müller, F.: Über Icterus, Jahresb. d. schles. Gesellsch. f. vaterland Cult. 70: 1-13, 1893. Google Scholar32. BillingLathe GHGH: The excretion of bilirubin as an ester glucuronide giving the direct van den Bergh reaction, Proc. Biochem. Soc., Biochem. J. 63: 6 p. 1956. Google Scholar33. Schmid R (a) : Direct-reacting bilirubin, bilirubin glucuronide in serum, bile and urine, Science 124: 76, 1956. (b) Schmid, R., Hammaker, L., and Axelrod, J.: The enzymatic formation of bilirubin glucuronide, Arch. Biochem. and Biophysics 70: 285, 1957. CrossrefMedlineGoogle Scholar34. Talafant E: Properties and composition of the bile pigment giving a direct diazo reaction, Nature, London 2: 312, 1956. CrossrefGoogle Scholar35. Netousek M: Über den Abbau des Blutfarbstoffes, Acta med. Acad. scient. Hungar. 9: 143, 1956. MedlineGoogle Scholar36. FelixMoebus KH: Das Verhalten von Urobilinogen in der Leber, Ztschr. f. d. ges. exper. Med. 85: 369, 1932. Google Scholar37. LondonWest IR: The formation of bile pigment in pernicious anemia, J. Biol. Chem. 184: 359, 1950. CrossrefMedlineGoogle Scholar38. Whipple G: Pigment metabolism and regeneration of hemoglobin in the body, Harvey Lectures 17: 95, 1921-2. Google Scholar39. Turner WW (a) : A theory of porphyrinogenesis, J. Lab. and Clin. Med. 26: 323, 1940. (b) Bogorad, L., and Granick, S.: The enzymatic synthesis of porphyrins from porphobilinogen, Proc. Nat. Acad. Sc. 39: 1176, 1953. Google Scholar40. SiedelPölnitzEisenreich WWF: Bilifuscin und Mesobilifuscin als natürliche Abbauprodukte des Blutfarbstoffes; über Vorkommen und Bildung, Naturwissensch. 34: 314, 1947. CrossrefGoogle Scholar41. SiedelStichEisenreich WWF: Promesobilifuscin (Mesobilileukan) ein neues physiologisches Abbauprodukt des Blutfarbstoffs, Naturwissensch. 35: 316, 1948. CrossrefGoogle Scholar42. Jedlicka V: Über die Lebertherapie und das Wesen der perniziösen Anämie, Folia haemat. 42: 359, 1930. Google Scholar43. Crosby WH: Normal functions of the spleen relative to red blood cells, Official Program, International Society of Hematology, VI Congress, 1956, p. 275. Google Scholar44. WatsonSchwartzSborovBertie CJSVE (a) : Studies of urobilinogen. V. A simple method for the quantitative recording of the Ehrlich reaction as carried out with urine and feces, Am. J. Clin. Path. 14: 605, 1944. (b) Watson, C. J., and Hawkinson, V.: Studies of urobilinogen. VI. Further experience with the simple quantitative Ehrlich reaction. Corrected calibration of the Evelyn colorimeter with a pontacyl dye mixture in terms of urobilinogen, Am. J. Clin. Path. 17: 108, 1947. CrossrefGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Minneapolis, Minnesota*Presented in part as the John Phillips Memorial Lecture at the Thirty-eighth Annual Session of The American College of Physicians, Boston, Massachusetts, April 8, 1957.From the Department of Medicine, University of Minnesota Hospital, Minneapolis, Minnesota.The studies from the author's laboratory mentioned in this paper were supported by the following agencies or individuals: The Graduate School of the University of Minnesota; the Surgeon General's Office, U. S. Army; the Atomic Energy Commission; the John and Mary Briggs Porphyria Research Fund, and the Louis Weinberg Fund for Research on Liver Disease.Requests for reprints should be addressed to Cecil J. Watson, M.D., 412 Delaware Street, Southeast, Minneapolis 14, Minnesota.†Historical aspects are considered in more detail in the author's E. T. Bell Lecture,1 which also includes certain topics pertaining to the general theme that are not included here. At the same time, the present discussion attempts to bring up to date some questions which were raised in the Bell Lecture. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byDerivatives of Bile PigmentsThe Continuing Challenge of Hemoglobin and Bile Pigment MetabolismC. J. WATSON, M.D., M.A.C.P.The Formation and Excretion of BilirubinDie Mesobilifuscinurie bei Blut- und LeberkrankheitenInnere Erkrankungen mit latenter Blutumsatzsteigerung 1 October 1957Volume 47, Issue 4Page: 611-625KeywordsAplastic anemiaHemeHemolytic anemiaHospital medicineLiver diseasesMemoryPorphyriaResearch fundingResearch laboratoriesSurgeons ePublished: 1 December 2008 Issue Published: 1 October 1957 Copyright & PermissionsCopyright ©, 1957, by The American College of PhysiciansPDF downloadLoading ...