tbuckley/cabot-search huggingface dataset:
Abstract 4765: Treatment with anti - IL 17 A coupled with COX-2 inhibitor significantly decreases breast cancer associated secondary metastasis in a model of autoimmune arthritis
Authors: Lopamudra Das Roy, Jorge L. Schettini, Helen E. Gruber, Mahnaz M. Sahraei, Pinku Mukherjee
Journal: Cancer Research (2010)

Abstract Introduction: Inflammation is a critical hallmark of autoimmune arthritis (AA) and cancer. We have previously shown that the site of chronic inflammation linked to AA creates a milieu that attracts tumor cells to home and grow in the inflamed bones and lungs which are frequent sites of breast cancer metastasis. We have identified IL-17, a critical pro-inflammatory cytokine involved in osteoclastic bone resorption in AA as the underlying mechanism for increased metastasis. In addition, high levels of cyclooxygenase 2 (COX-2) is linked to both AA and breast cancer metastasis. We report that blocking the IL-17 and COX-2 pathways simultaneously significantly reduces the development of breast cancer associated metastasis in a spontaneous model of AA. Methods: 4T1 mammary gland tumors were generated in mice genetically prone to develop AA (designated SKG mice). When tumors reached &amp;gt; 0.2 gms, anti-IL17 antibody treatment was injected intraperitoneally once a week for three weeks. Celecoxib, a specific COX-2 inhibitor was orally administered starting at the same time as the anti-IL-17 antibody but was given daily until sacrifice. One week after the last injection, mice were sacrificed. Secondary metastasis and the associated inflammatory milieu were evaluated. Summary of Results: Data demonstrated that compared to the untreated mice or mice treated with isotype control antibody, survival was significantly enhanced and primary tumor burden was significantly decreased in the anti-IL-17+celecoxib treated mice. Importantly, we observed significant decrease in lung and bone metastasis as compared to the control group. The contributing factor was recognized to be the low neutrophilic and granulocytic infiltration and therefore low inflammation in the lungs as well as reduced severity of arthritis with low cellular infiltration and low osteoclast formation in the bones of mice treated with anti-IL-17+celecoxib. Conclusion: We report that treatment with anti-IL 17 and celecoxib significantly reduced primary tumor burden, reduced metastasis to the lungs and bones, and increased survival in SKG mice. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4765.
Adaptation of the Exocrine Pancreas to Diet
Authors: P. M. Brannon
Journal: Annual Review of Nutrition (1990)

Pancreatic adaptation represents dietary regulation of gene expression; dietary substrates alter the synthesis and mRNA levels of their respective digestive enzymes. The mechanisms whereby mRNA levels change are not understood, but they must be elucidated. Although the changes in synthesis of proteases, amylase, and lipase parallel the changes in their mRNA levels in response to respective substrates, the concomitant changes in the synthesis of the other enzymes can be discordant with mRNA levels. The evidence supports a pretranslational mechanism of the adaptation of proteases, amylase, and lipase to their respective substrates and suggests potential translational mechanisms of other enzymes in these adaptations. Changes in synthesis occur within hours after a dietary change, but whether mRNA levels also change so early is unknown. Rapid, adaptive changes may occur by a different mechanism from later adaptation, possibly by translational control or nuclear transport. The differential effects of acute and chronic caerulein administration support the possibility of multiple mechanisms of regulation by a single effector. The mediators of pancreatic adaptations have not yet been identified, except for adaptation to dietary protein. CCK appears to mediate protease adaptation through the feedback regulation of its release by dietary protein. Available evidence supports a role of insulin and glucose in the adaptation to carbohydrate and a role of secretin and ketones in the adaptation to dietary fat. Elucidation of the mediators of pancreatic adaptation to carbohydrate and fat and their mechanisms is needed.
Pd‐Porphyrin‐Cross‐Linked Implantable Hydrogels with Oxygen‐Responsive Phosphorescence
Authors: Haoyuan Huang, Wentao Song, Guanying Chen, Justin M. Reynard, Tymish Y. Ohulchanskyy, Paras N. Prasad, Frank V. Bright, Jonathan F. Lovell
Journal: Advanced Healthcare Materials (2013)

Development of long‐term implantable luminescent biosensors for subcutaneous oxygen has proved challenging due to difficulties in immobilizing a biocompatible matrix that prevents sensor aggregation yet maintains sufficient concentration for transdermal optical detection. Here, Pd‐porphyrins can be used as PEG cross‐linkers to generate a polyamide hydrogel with extreme porphyrin density (≈5 × 10 −3 m ). Dye aggregation is avoided due to the spatially constraining 3D mesh formed by the porphyrins themselves. The hydrogel exhibits oxygen‐responsive phosphorescence and can be stably implanted subcutaneously in mice for weeks without degradation, bleaching, or host rejection. To further facilitate oxygen detection using steady‐state techniques, an oxygen‐non‐responsive companion hydrogel is developed by blending copper and free base porphyrins to yield intensity‐matched luminescence for ratiometric detection.
A5.14 Disease-regulated local interleukin-10 gene therapy diminishes synovitis and articular cartilage damage in experimental arthritis
Authors: Eline A Vermeij, Mathijs G A Broeren, Miranda B Bennink, Onno J Arntz, Inger Gjertsson, Wim B van den Berg, Fons A J van de Loo
Journal: Annals of the Rheumatic Diseases (2014)

<h3>Background and Objectives</h3> Rheumatoid arthritis (RA) is a chronic destructive autoimmune disease with periods of exacerbation and remission. An attractive treatment would provide a disease-regulated therapy that offers flexible drug delivery. Therefore, we expressed the anti-inflammatory interleukin-10 (IL-10) gene under the control of an inflammation-dependent promoter in a mouse model of RA. <h3>Materials and Methods</h3> Proximal promoters of S100a8, Cxcl1, Mmp13, Saa3, IL-1b, and TSG6 were selected from endogenous genes differentially regulated in the inflamed synovium of arthritic mice. Mice were injected intra-articularly in kneejoints with lentiviral vectors expressing a luciferase reporter or the therapeutic protein IL-10 under control of selected promoter. After 4 days, arthritis was induced by intra-articular injection of streptococcal cell walls. At different timepoints after arthritis induction, <i>in-vivo</i> bioluminescent imaging was performed or knee joints were dissected for histological and RNA analysis. <h3>Results</h3> The disease-regulated promoters showed different activation profiles during the course of the disease, subsequently the Saa3 and MMP13 promoter were selected for further research, because of differences in activation at day 1. Overexpression of IL-10 resulted in less synovitis and cartilage proteoglycan (PG) depletion and in upregulation of IL-1Ra and SOCS3 gene expression. IL-1Ra counteracts the detrimental effects of IL-1 on cartilage damage and SOCS3 inhibits the JAK/STAT pathway and subsequent inflammation; this can explain the diminished synovitis and PG depletion. Probably because IL-10 is expressed at day 1 of arthritis without any treatment, major therapeutic differences between the MMP13 and SAA3 promoter were not evident in this study. <h3>Conclusion</h3> We can conclude that local inflammation-dependent IL-10 gene therapy suppresses experimental arthritis and is a promising strategy in the development of novel treatments for RA.
Ambiguous Heart Murmurs Clarified by Echocardiogram
Authors: Bahram Eslami
Journal: JAMA (1974)

Echocardiograms provided the basis for complete diagnoses of the following conditions: (1)<i>idiopathic hypertrophic subaortic stenosis</i>in five patients in the absence of typical physical findings; (2)<i>left atrial myxoma</i>in two patients with clinical features of mitral stenosis, and<i>right atrial myxoma</i>in a patient with a heart murmur (one of a total of five); (3)<i>right ventricular volume</i>overload in three patients with atrial septal defect and one patient with tricuspid regurgitation; (4) combined<i>mitral and aortic valve disease</i>in three patients. In five other patients with mitral stenosis unsuspected aortic stenosis was demonstrated. Echocardiography is a valuable aid in cardiac diagnosis. (<i>JAMA</i>230:873-876, 1974)
Association between pediatric psoriasis and the metabolic syndrome
Authors: Shiu-chung Au, Ari M. Goldminz, Daniel S. Loo, Nicole Dumont, Danielle Levine, Eva Volf, Melissa Michelon, Andrew Wang, Noori Kim, Shimrat Yaniv, Paul F. Lizzul, Todd Kerensky, Jorge M. Lopez-Benitez, Marc Natter, Laurie Miller, Christina F. Pelajo, Trevor Davis, Alice B. Gottlieb
Journal: Journal of the American Academy of Dermatology (2012)
Replantation of Portions of Four Fingers in One Hand
Authors: Boonmee Chunprapaph
Journal: New England Journal of Medicine (1974)

SINCE the first successful replantation of a human thumb reported by Komatsu and Tamai1 in 1968, hundreds of fingers have been successfully replanted, principally in the People's Republic of China, with a 50 per cent success rate.2 Replantation of multiple fingers in one hand is rarer, and successful replantation of all four fingers in one hand rarer still. Snyder et al.3 described a patient in whom four digits were replanted without success. Ch'en2 also replanted four fingers, with survival of three.The following case of successful replantation of portions of four fingers demonstrates three technics of digital reattachment: arterial and . . .
Selective expression of histamine receptors H1R, H2R, and H4R, but not H3R, in the human intestinal tract
Authors: L E Sander
Journal: Gut (2005)

Histamine is known as a regulator of gastrointestinal functions, such as gastric acid production, intestinal motility, and mucosal ion secretion. Most of this knowledge has been obtained from animal studies. In contrast, in humans, expression and distribution of histamine receptors (HR) within the human gastrointestinal tract are unclear.We analysed HR expression in human gastrointestinal tissue specimens by quantitative reverse transcription-polymerase chain reaction and immunostaining.We found that H1R, H2R, and H4R mRNA were expressed throughout the gastrointestinal tract, while H3R mRNA was absent. No significant differences in the distribution of HR were found between different anatomical sites (duodenum, ileum, colon, sigma, and rectum). Immunostaining of neurones and nerve fibres revealed that H3R was absent in the human enteric nervous system; however, H1R and H2R were found on ganglion cells of the myenteric plexus. Epithelial cells also expressed H1R, H2R and, to some extent, H4R. Intestinal fibroblasts exclusively expressed H1R while the muscular layers of human intestine stained positive for both H1R and H2R. Immune cells expressed mRNA and protein for H1R, H2R, and low levels of H4R. Analysis of endoscopic biopsies from patients with food allergy and irritable bowel syndrome revealed significantly elevated H1R and H2R mRNA levels compared with controls.We have demonstrated that H1R, H2R and, to some extent, H4R, are expressed in the human gastrointestinal tract, while H3R is absent, and we found that HR expression was altered in patients with gastrointestinal diseases.
Effects of Temporal-Lobe Surgery on Behavior
Authors: Norman Geschwind
Journal: New England Journal of Medicine (1973)

When Mr. Murray Falconer moved from New Zealand to London, his neurosurgical unit based jointly in two major London teaching hospitals and at the Maudsley Hospital, one of the leading psychiatric centers of the world, began a methodical study of surgery in the control of temporal-lobe epilepsy. Although the primary indication for surgery was uncontrolled seizures, the presence of behavioral changes in a large fraction of the 250 patients operated on has permitted the accumulation of considerable data on the clinical pictures of these personality changes and the effects of surgery. This study has had the advantage of independent assessment . . .
Differential association of calreticulin type 1 and type 2 mutations with myelofibrosis and essential thrombocytemia: relevance for disease evolution
Authors: X Cabagnols, J P Defour, V Ugo, J C Ianotto, P Mossuz, J Mondet, F Girodon, J H Alexandre, O Mansier, J F Viallard, E Lippert, A Murati, M J Mozziconacci, P Saussoy, M C Vekemans, L Knoops, F Pasquier, V Ribrag, E Solary, I Plo, S N Constantinescu, N Casadevall, W Vainchenker, C Marzac, O Bluteau
Journal: Leukemia (2014)
The Changing Face of Medicine
Authors: R. Young
Journal: BMJ (1950)

MEDI-AL-JOUIRNALprovided always that I personally superintended every detail of the arrangements.We relied on the resources of the household for basins, linen, and furniture.Many a baking-bowl a,cted as a reservoir for Lister's strong mixture in which to soak towels clean from the wash, and usually an ashet (meat dish) did very well as an instrument-tray.Some surgeons carried a lot of equipment in the way of portable operating table, small stands, basins, dishes, linen, etc., but most of us carried only a Gladstone bag or some- thing rather larger for holding the instruments.The general practitioner usually gave the anaesthetic, and one of his friends sometimes lent a hand as assistant.In emergencies we usually had to manage without a nurse, though when district nurses were available-and did not faint-they were invaluable.Sometimes a " handy body " had to suffice, or we did without.In the chloroform days the anaesthetic might be an anxiety, but as often as not this was because the administrator was too timid and the patient would not relax or would want to get off the table.At night illumination was a great trouble: a single gas flame or an oil lamp is a really poor source of light, and no one knows how feeble is the light from candles unless he has tried to do a strangu- lated hernia or remove an appendix with their aid.Nursing-homes were starting, but they were just homes, and at that time none was properly equipped for the purpose.That is why so many surgeons were driven to set up their own private hospitals, thus adding to their responsibilities and anxieties.
LOUPING ILL IN MAN
Authors: Thomas M. Rivers, Francis F. Schwentker
Journal: The Journal of Experimental Medicine (1934)

Four instances of infection in man which are believed, because of the circumstances under which they occurred and in view of the results of neutralization tests, to represent cases of louping ill have been described. Evidence obtained by the neutralization tests is in favor of the idea that the antibodies against louping ill virus demonstrated in certain sera were most likely the result either of contact with or of infection with the active agent.
A total audit of preventive procedures in 45 practices caring for 430,000 patients.
Authors: M Lawrence, A Coulter, L Jones
Journal: BMJ (1990)

OBJECTIVE--To develop and report the results of a system of audit of computer records in general practice. DESIGN--A retrospective audit of records in practices using the same computer system. Information about recorded preventive procedures was collected by sending the same audit program to each practice on floppy disk. Other characteristics of the practices were determined by postal questionnaire. SETTING--Forty five general practices, widely distributed in England and Wales. SUBJECTS--All 430,901 patients registered with the practices. MAIN OUTCOME MEASURES--Within each practice the percentage of patients in specified age groups for whom certain preventive procedures were recorded as having been carried out. These measures were analysed in relation to practice characteristics. RESULTS--Practice characteristics and recording rates for preventive procedures varied over a wide range. Recording rates were higher in practices with computer terminals on every doctor9s desk. Only one practice achieved the new contract target of 90% coverage for recorded primary immunisations, and fewer than two thirds recorded 80% coverage for cervical cytology in the past five years. Practices holding clinics did no better than those without. Smaller partnerships and smaller doctors9 list sizes were associated with better performance. CONCLUSIONS--Centrally programmed audit of computerised records is a feasible method of providing data on a regular basis for epidemiological purposes and for performance review. The fact that practices with smaller list sizes had higher levels of recorded preventive care suggests that the trend towards larger lists promoted by the new contract might militate against the intended effect of better preventive care.
SYNERGY AMONG LYMPHOID CELLS MEDIATING THE GRAFT-VERSUS-HOST RESPONSE
Authors: Harvey Cantor, Richard Asofsky
Journal: The Journal of Experimental Medicine (1970)

The capacity of cells from different lymphoid tissues obtained from Balb/c mice to produce graft-vs.-host (GVH) reactions was quantitatively determined in C57BL/6N by Balb/c F(1) hybrid recipients. Synergistic responses were observed when small numbers of cells from lymphoid tissues that were rich in GVH activity such as spleen and femoral lymph node were combined with weakly reactive thymus cells. Thymus and spleen cells obtained from 1-wk old mice were separately inactive but produced moderate GVH reactions when combined in equal proportions. GVH activity of spleen cells from mice thymectomized at 3 days of age was partially restored by the addition of small numbers of spleen or thymus cells from adult mice. Changes in ratio between the two cell populations markedly affected the degree of synergy. Synergy was not observed when Balb/c cells were combined with Balb/c x C57BL/6N F(1) hybrid cells and inoculated into C57BL/6N recipients, but was demonstrated when Balb/c and C57BL/6N cells were combined and inoculated into F(1) recipients, indicating that a genetic disposition to mount GVH reactions in both populations is required to produce synergy. The data indicate that at least two cell types are necessary for GVH reactions, and that synergy between cell populations results from favorable adjustments in the ratio between these two cell types.
Spatial control of adult stem cell fate using nanotopographic cues
Authors: Eun Hyun Ahn, Younghoon Kim, None Kshitiz, Steven S. An, Junaid Afzal, Suengwon Lee, Moonkyu Kwak, Kahp-Yang Suh, Deok-Ho Kim, Andre Levchenko
Journal: Biomaterials (2014)
Pancreatitis: An Evaluation of a Trypsin Inhibitor.
Authors: G. Gordon McHardy
Journal: Annals of Internal Medicine (1964)

s1 February 1964Pancreatitis: An Evaluation of a Trypsin Inhibitor.G. Gordon McHardy, M.D., F.A.C.P., Claude C. Craighead, M.D., Louis Balart, M.D., Robert J. McHardy, M.D. (Associate), Clinton La Grange, B.S., Donald Bradburn, M.D., Harold Cradic, B.S.G. Gordon McHardy, M.D., F.A.C.P.Search for more papers by this author, Claude C. Craighead, M.D.Search for more papers by this author, Louis Balart, M.D.Search for more papers by this author, Robert J. McHardy, M.D. (Associate)Search for more papers by this author, Clinton La Grange, B.S.Search for more papers by this author, Donald Bradburn, M.D.Search for more papers by this author, Harold Cradic, B.S.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-60-2-325_3 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe pathogenesis of pancreatitis is not clearly defined. Therapeutic limitation and a high case fatality rate warrant reevaluation of experimentally induced pancreatitis as modified by a trypsin inhibitor in an effort to establish its possible specificity in the management of pancreatitis in humans.The first objective was the confirmatory reproduction of acute pancreatitis, creating intrapancreatic proteolytic trypsin activity in mongrel dogs.The second objective was an evaluation of a trypsin and kallikrein inhibitor as a protection against such experimentally produced pancreatitis. The trypsin inhibitor used was that isolated from the parotid gland of cattle with in vitro potency of 1:4... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: New Orleans, Louisiana PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 February 1964Volume 60, Issue 2_Part_1Page: 325-326KeywordsAcute pancreatitisPancreatitisParotid glandsPathogenesisSpecificity Issue Published: 1 February 1964 PDF DownloadLoading ...
214 Intrasplenically transplanted human hepatic stellate cells (HSC) engraft and survive in the liver of immunodeficient mice for prolonged periods
Authors: D BENTEN, V KUMARAN, B JOSEPH, D BRENNER, S GUPTA
Journal: Hepatology (2003)

Many different tumor cell types (breast, ovarian, glioma, liver and colon) were retrovirally transduced with the human macrophage colony stimulating factor (M-CSF) gene (either the membrane associated form [mM-CSF] or the secreted form [sM-CSF]). These cells were tested for their ability to display increased amounts of mM-CSF in response to dexamethasone. M-CSF-transfected tumor cells expressed additional mM-CSF in response to 18–72 h incubations with 3–15 μg/ml dexamethasone, while non-transfected parental cells were unaffected by this treatment. Increased mM-CSF protein expression on the M-CSF transduced cells was observed by flow cytometry and Western blotting using M-CSF specific antibodies. Northern blot analysis revealed an increase in the mM-CSF specific transcripts within the dexamethasone-treated mM-CSF transduced cells, but this was not seen within the non-transfected tumor cells that were treated with dexamethasone. ICAM-1 expression was unaffected by dexamethasone treatment, indicating that this response is mM-CSF specific. All trans-retinal and 1,25-dihydroxy vitamin D3 compounds that have been reported to induce M-CSF expression failed to increase mM-CSF. When dexamethasone-treated mM-CSF transfected clones were used as target cells for macrophage-mediated cytotoxicity assays, an increased killing with the dexamethasone-treated cells was seen. The macrophage-mediated cytotoxicity of these mM-CSF expressing tumor cells was blocked with excess recombinant M-CSF by saturating M-CSF receptors on the macrophage that is required for this form of tumor cell killing. This work suggests the possibility that dexamethasone may prove useful for vaccination purposes using mM-CSF retrovirally transfected tumor cells.
Replantation of an Infant's Arm
Authors: Jens G. Rosenkrantz, Robert C. Sullivan, Keasley Welch, James S. Miles, Keith M. Sadler, Bruce C. Paton
Journal: New England Journal of Medicine (1967)

SINCE the first successful replantation of a human arm in 19621 reports of similar cases have appeared.2 3 4 5 6 7 Success after replantation near the shoulder has been limited by varying degrees of neurologic deficit. The patient described in this paper, considerably younger than those in previous reports, demonstrated some of the hazards of replantation of extremities. However, her remarkable degree of reinnervation confirms a clinical impression that the indications for replantation should be more liberal in children than in adults.Case ReportA 21-month-old, 9.8-kg. girl was admitted to the hospital on April 10, 1965, approximately 20 minutes after amputation of the . . .
Urinary secretion and extracellular aggregation of mutant uromodulin isoforms
Authors: Céline Schaeffer, Angela Cattaneo, Matteo Trudu, Sara Santambrogio, Ilenia Bernascone, Daniela Giachino, Gianluca Caridi, Andrea Campo, Corrado Murtas, Simona Benoni, Claudia Izzi, Mario De Marchi, Antonio Amoroso, Gian Marco Ghiggeri, Francesco Scolari, Angela Bachi, Luca Rampoldi
Journal: Kidney International (2012)