tbuckley/cabot-search huggingface dataset:
Transmission Electron Microscopy Surveillance of Retroviruses in Tissue Culture Cells Prepared by the Critical-Point Drying Method&lt;xref ref-type="fn" rid="FN2"&gt;2&lt;/xref&gt;
Authors: Yuzo Iwasaki, Nobuo Tsuchida
Journal: JNCI Journal of the National Cancer Institute (1978)

Tissue culture cells grown on grids were processed by the critical-point drying whole-cell method. With the use of a conventional transmission electron microscope operating at 100 kV, this technique permitted visualization of intracytoplasmic organelles of unsectioned whole cells. The morphology of type C virus in the process of budding and also in extracellular locations closely resembled that revealed in thin sections. Preliminary results of virus surveillance of tissue culture cells prepared by this technique was corroborated by the levels of reverse transcriptase activity in culture media and by immunofluorescence staining of viral antigens on the cell surface.
Keller's Operation with Basal Osteotomy of First Metatarsal
Authors: R L Allum, D W Higginson
Journal: Journal of the Royal Society of Medicine (1983)

A retrospective survey was carried out to assess the results in the middle aged and elderly of an operation for hallux valgus which combined a basal osteotomy of the first metatarsal with a Keller's procedure. Mean follow up in 34 patients (54 feet) was 3.2 years. The correction of both the hallux valgus and the varus angle of the first metatarsal was found to be satisfactory. The advantages and disadvantages of the operation are discussed.
Transplantation of Human Tumors in Nude Mice23
Authors: Yukio Shimosato, Toru Kameya, Kanji Nagai, Setsuo Hirohashi, Tsutomu Koide, Hiroatsu Hayashi, Tatsuji Nomura
Journal: JNCI Journal of the National Cancer Institute (1976)

Journal Article Transplantation of Human Tumors in Nude Mice Get access Yukio Shimosato, Yukio Shimosato Search for other works by this author on: Oxford Academic PubMed Google Scholar Toru Kameya, Toru Kameya Search for other works by this author on: Oxford Academic PubMed Google Scholar Kanji Nagai, Kanji Nagai Search for other works by this author on: Oxford Academic PubMed Google Scholar Setsuo Hirohashi, Setsuo Hirohashi Search for other works by this author on: Oxford Academic PubMed Google Scholar Tsutomu Koide, Tsutomu Koide Search for other works by this author on: Oxford Academic PubMed Google Scholar Hiroatsu Hayashi, Hiroatsu Hayashi Search for other works by this author on: Oxford Academic PubMed Google Scholar Tatsuji Nomura Tatsuji Nomura Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 56, Issue 6, June 1976, Pages 1251–1260, https://doi.org/10.1093/jnci/56.6.1251 Published: 01 June 1976 Article history Received: 22 September 1975 Accepted: 10 December 1975 Published: 01 June 1976
Effects of Hedgehog Signaling Inhibition on Epithelial-Stromal Interactions in Triple Negative Breast Cancer Cells
Authors: A. Servetto, L. Raimondo, L. Formisano, R. Marciano, C. Di Mauro, R. Rosa, V. D'Amato, B.M. Veneziani, S. De Placido, R. Bianco
Journal: Annals of Oncology (2014)
Abstract 288: Rnd3/RhoE Regulates Cardiac Ryanodine Receptor Type 2 Stability
Authors: xiangsheng yang, Tiannan Wang, Xiaojing Yue, Xander H.T. Wehrens, Jiang Chang
Journal: Circulation Research (2014)

Rationale: Rnd3, a small Rho GTPase, is involved in the regulation of cell actin cytoskeleton dynamics, cell migration and proliferation. The biological function of Rnd3 in the heart remains unexplored. Objective: To define the functional role of the Rnd3 gene in the animal heart and investigate the associated molecular mechanism. Methods and Results: By loss-of-function approaches, we discovered a new role in which Rnd3 stabilizes the ryanodine receptor type 2 (RyR2) Ca 2+ release channel. Genetic deletion of Rnd3 in mice resulted in embryonic lethality with heart failure and arrhythmia. Both Rnd3 -/- embryonic and Rnd3 +/- adult cardiomyocytes showed severe Ca 2+ leakage. Single channel assessment showed the destabilized RyR2 channel, and this irregular spontaneous Ca 2+ release was curtailed by protein kinase A (PKA) inhibitor treatment. Further studies found that RyR2 protein was hyperphosphorylated by PKA in the mutant heart. Remarkable increases in the PKA activity along with elevated cyclic adenosine monophosphate levels were detected in vivo in Rnd3-null embryos and in vitro in neonatal rat cardiomyocytes and non-cardiac cell lines with Rnd3 knockdown. Moreover, we found increasing β 2 -adrenergic receptor (β 2 AR) protein levels, but no correlated mRNA changes in both the Rnd3-null heart and non-cardiac cells with Rnd3 knockdown. Immunoprecipitation analysis demonstrated that Rnd3 and β 2 AR physically interacted. Multiple post-translational modification analyses of β 2 AR revealed that downregulation of Rnd3 attenuated β 2 AR protein lysosomal targeting and ubiquitination, which in turn resulted in the elevation of β 2 AR protein levels contributing to the activation of PKA signaling. Rnd3 deficiency had no effects on the hydroxylation- and sumoylation-mediated β 2 AR protein degradation. Conclusion: Rnd3 is a unique stabilizer of RyR2 that impacts intracellular Ca 2+ handling in the heart.
Effect of wound infiltration with ropivacaine or bupivacaine analgesia in breast cancer surgery: A meta-analysis of randomized controlled trials
Authors: Ka-Wai Tam, Shin-Yan Chen, Tsai-Wei Huang, Chao-Chun Lin, Chih-Ming Su, Ching-Li Li, Yuan-Soon Ho, Wan-Yu Wang, Chih-Hsiung Wu
Journal: International Journal of Surgery (2015)
Cytoplasmic Inclusion Bodies in Primary and Transplanted Hepatomas of Mice of Different Strains&lt;xref ref-type="fn" rid="FN2"&gt;2&lt;/xref&gt;
Authors: Annabel Glockler Liebelt, Robert A. Liebelt, Leon Dmochowski
Journal: JNCI Journal of the National Cancer Institute (1971)

Eosinophilic cytoplasmic inclusion bodies of specific morphology were seen in hepatomas arising in mice of certain strains. They were found consistently in strains C3H, C3Hf, and C57BL, never in the CBA strain, and only occasionally in mice of other strains. This type of inclusion body was not seen in normal liver or livers with other pathology. The inclusion bodies were not usually found after one or two transplant passages of the hepatomas and were never found if morphology of the tumor changed to another cell type. These spherical structures ranged from 0.1–12.0 μ. Cytochemical analyses and electron microscopic observations indicated that they contained a high concentration of protein. Acid phosphatase procedures gave negative results.
Clinical role of pleural effusion MMP-3 levels in malignant pleural mesothelioma
Authors: Chiharu Tabata, Aki Murakami, Rie Tabata, Hisaya Okuwa, Takashi Nakano
Journal: European Respiratory Journal (2012)

Malignant pleural mesothelioma (MPM) is an aggressive malignant tumor of mesothelial origin associated with asbestos exposure. MPM displays a limited response to conventional chemotherapy and radiotherapy, so early diagnosis of MPM is very important. Malignant tumor progression requires destruction of the basement membrane (BM), which is constructed from extracellular matrix (ECM) materials. Matrix metalloproteinase (MMP) are thought to be important due to their wide degrading function. We investigated the pleural effusion MMP-3 levels of patients with MPM and compared them to those of a population with non-malignant pleuritis or lung cancer involving malignant pleural effusion. The pleural effusion MMP-3 concentrations of 52 MPM patients and 67 non-MPM patients were measured. We demonstrated that the MPM patients had significantly higher pleural effusion MMP-3 levels than the population with non-malignant pleuritis.  The overall survival of the MPM patients with lower pleural effusion MMP-3 levels was longer than that of those with higher pleural effusion MMP-3levels.  Our data suggest clinical role of pleural effusion MMP-3 levels in malignant pleural mesothelioma.
Effects of respiratory muscle stretching on thoracoabdominal mechanics, functional capacity and dyspnea in COPD patients
Authors: Juliano Wada, Erickson Borges-Santos, Ronaldo Aparecido Silva, Desiderio Cano Porras, Denise Paisani, Cibeli Marques Silva, Alberto Cukier, Rafael Stelmach, Adriana Claudia Lunardi, Celso Ricardo Fernandes Carvalho
Journal: European Respiratory Journal (2014)

COPD patients have an increased recruitment of inspiratory muscles predisposing to a chest wall incoordination, modifying thoracoabdominal mechanics and leading to poor exercise capacity and dyspnea.  Aim : To assess the effects of respiratory muscle stretching on thoracoabdominal mechanics, functional capacity and dyspnea in COPD patients.  Methods : 26 patients moderate to severe (63±6yrs, 25.8±3.4kg/m 2 , FEV 1 =44±13%Pred) were randomly allocated into Control (CG; n=12) or Treatment (TG; n=14) group. Both groups performed aerobic training during 24 sessions. In addition, TG received chest wall muscle stretching and CG peripheral muscle stretching. T test was used to compare the change (Δ=post–pre) between groups. Before and after intervention was evaluated: volume in the chest wall and each compartments (upper (Vuc) and lower chestwall (Vlc) and abdomen (Vabd)) using optoelectronic plethysmography and inspiratory muscle activation using surface electromyography during exercise. Expiratory flow limitation (EF), 6 minute walking test (6MWT) and dyspnea (Borg scale) were also assessed.  Results : Compared to CG, TG presented an increase chest wall volume that occurred mainly due to increase in the Vabd (p=0.001) and also a reduction on recruitment of inspiratory muscle activation (p=0.006). TG also presented a clinically significant increase on 6MWT (25.4m) and a decrease on BORG scale (3 points) and EF when compared to CG (p Conclusion : Respiratory muscle stretching increases the ventilatory capacity that was associated with a reduction on the respiratory muscle activity. An increase on functional capacity and reduction on dyspnea was also observed.
Characterization of Mouse Mammary Tumor Viruses From Primary Tumor Cell Cultures. II. Biochemical and Biophysical Studies 2
Authors: P. C. Kimball, R. Michalides, D. Colcher, J. Schlom
Journal: JNCI Journal of the National Cancer Institute (1976)

Primary mammary tumor cultures of RIII, GR, DD, BALB/c, and BALB/cfC3H mice were examined for mouse mammary tumor virus (MuMTV) production. Levels of production of 12–32 μg virus protein/day/75-cm2 culture flask could be maintained for 30–50 days with daily virus harvests. The viruses from tumor cell cultures of these mouse strains contained DNA polymerase with a strong preference for Mg++ over Mn++ as the divalent cation, a characteristic of DNA polymerase of MuMTV from mouse milk. These viruses from tumor cell cultures were excellent sources of MuMTV 3H-complementary DNA (complexed to 60–70S RNA) and radioactive 60–70S RNA, sufficiently free of contaminating murine leukemia virus nucleic acids, that can be used in molecular hybridization experiments. The effects of several culture parameters on MuMTV production were also studied.
Lack of relationship between systemic exposure for the component drug of the fluorouracil, epirubicin, and 4-hydroxycyclophosphamide regimen in breast cancer patients.
Authors: M Sandström, A Freijs, R Larsson, P Nygren, M L Fjällskog, J Bergh, M O Karlsson
Journal: Journal of Clinical Oncology (1996)

PURPOSE The aim of this study was to investigate the covariance between the pharmacokinetics of the three components of the FEC regimen, epirubicin (EPI), fluorouracil (5-FU), and the cyclophosphamide (CP) metabolite 4-hydroxycyclophosphamide (4-OHCP), in breast cancer patients. PATIENTS AND METHODS Data from 21 women were collected over a total of 35 cycles. 5-FU (300 to 600 mg/m2) and CP (300 to 600 mg/m2) were administered as bolus injections, whereas EPI (15 to 60 mg/m2) was administered either as a bolus injection or as an infusion. The pharmacokinetics of the component drugs were monitored using a limited sampling scheme. Population pharmacokinetic models for each of the three drugs were developed using the program NONMEM. RESULTS The data for 5-FU were best described by a one-compartment model with nonlinear elimination, where the maximal rate of elimination (Vmax) and the concentration at which the elimination was half-maximal (Km) were 105 mg/L.h and 27 mg/L, respectively. EPI concentration-time profiles showed a triexponential decline, with a mean terminal half-life of 24 hours and a clearance (CL) of 59 L/h. The elimination of 4-OHCP was monoexponential, with a mean half-life of 7 hours. The interindividual coefficients of variation (CVs) in CL were 30%, 22%, and 41% for 5-FU, EPI, and 4-OHCP, respectively. The corresponding values for intrapatient course-to-course variability in CL were 11%, 8%, and 27%. No significant correlation in any of the pharmacokinetic parameters between the drugs was found. CONCLUSION Individualization of dosing of the FEC regimen using therapeutic drug monitoring and attempts to find concentration-response relationships may be successful, but requires that the exposure of all three drugs is considered simultaneously.
Catatonia: prediction of response to somatic treatments
Authors: R Abrams, M A Taylor
Journal: American Journal of Psychiatry (1977)

The authors investigated predictors of somatic treatment response in 55 patients with one or more of eight catatonic motor features. Responders more often had good prognostic signs, rapid or pressured speech, and diagnosable affective disorder or alcoholism. Nonresponders were younger at age of onset of first illness and were more frequently disoriented; they included all patients diagnosed as schizophrenic. The authors suggest that treatment response in catatonia is a function of primary diagnosis and that the syndrome is diagnostically nonspecific but occurs most often in patients with affective disorders.
Fractures of the thoracolumbar spine in major trauma patients
Authors: R. McGlone, S. Walters
Journal: BMJ (1999)

Editor—Meek is right to highlight the potential danger of missing fractures of the thoracolumbar spine in patients with trauma if the need for x ray investigations is based purely on the clinical examination. But he is wrong to say that the Advanced Trauma Life Support Manual for Physicians “does not recognise the importance of a distracting injury in masking the symptoms and signs of spinal fracture.”1

I believe that the quotation he gave was from the introductory section of the chapter on spinal trauma. The following quotes are from the same chapter.2 “Any patient with an altered level of consciousness or cognitive dysfunction (GCS <15), multisystem injuries, ... requires spinal protection until AP [anteroposterior] and lateral [thoracolumbar] spine x-rays are obtained to exclude any injury.” “In the secondary survey ... thoracolumbar spine films may be obtained ... if the mechanism of injury suggests the possibility of spinal injury.” “Other injuries may mask the physical findings of spinal injuries, which may go unsuspected unless the doctor obtains the appropriate x-rays.”

The work of Cooper et al quoted in the article is also referenced at the end of the relevant chapter in the manual.3
Significance of epidermal growth factor receptor in advanced ovarian cancer.
Authors: G Scambia, P Benedetti Panici, F Battaglia, G Ferrandina, G Baiocchi, S Greggi, R De Vincenzo, S Mancuso
Journal: Journal of Clinical Oncology (1992)

PURPOSE The purpose of this study was to investigate the significance of epidermal growth factor receptor (EGF-R) expression in a group of advanced ovarian carcinomas. PATIENTS AND METHODS The study was conducted on 72 previously untreated patients with International Federation of Gynecology and Obstetrics (FIGO) stage III-IV disease. The median follow-up was 24 months (range, 4 to 75 months). EGF-R was measured by a radioreceptorial assay. A cutoff of 1.5 fmol per milligram of protein was chosen to define EGF-R positivity. Medians and life tables obtained with the Kaplan and Meier method were analyzed by the log-rank test. The risk of progression was estimated by Cox's proportional hazards model. RESULTS EGF-R was detected in 54% of primary tumors. When EGF-R was analyzed in different tissue specimens of the same tumor, consistent findings were noted in 88% (seven of eight) of cases. A lower concordance rate (nine of 15; 60%) was found between primary tumors and omental metastases, with a tendency toward higher EGF-R levels in the latter. The EGF-R expression did not significantly correlate with age, stage, grading, and residual tumor after primary surgery. In the univariate analysis, stage IV disease, postoperative residual tumor diameter greater than 2 cm, presence of ascites, and EGF-R positivity were found to be significantly associated with a greater risk of disease progression. In the multivariate analysis, only the postoperative residual tumor and the EGF-R expression remained significantly associated with a high risk of progression. CONCLUSION Data reported here suggest that the presence of EGF-R in advanced ovarian tumor at the time of the primary surgery identifies a subset of patients with a particularly poor prognosis.
Cyclophosphamide, Doxorubicin, Vincristine, Prednisone Dose Intensification With Granulocyte Colony-Stimulating Factor Markedly Depletes Stem Cell Reserve for Autologous Bone Marrow Transplantation
Authors: Arnold Freedman, Donna Neuberg, Peter Mauch, John Gribben, Robert Soiffer, Kenneth Anderson, Michael Robertson, David C. Fisher, Robert Schlossman, Mary Kroon, Catherine Rhuda, Caroline Kuhlman, Jerome Ritz, Lee Nadler
Journal: Blood (1997)
Anthrapyrazole CI941: a highly active new agent in the treatment of advanced breast cancer.
Authors: D C Talbot, I E Smith, J L Mansi, I Judson, A H Calvert, S E Ashley
Journal: Journal of Clinical Oncology (1991)

Thirty-one patients with advanced breast cancer were treated with CI941, an anthrapyrazole structurally related to mitoxantrone. Patients had not previously been treated with anthracyclines or mitoxantrone, and 15 patients had not received any previous cytotoxic chemotherapy. CI941 was given at a dose of 50 mg/m2 by intravenous bolus injection every 21 days. Thirty patients were assessable for response, and all were assessable for toxicity. Two patients (7%) had complete responses (CRs), and 17 (56%) achieved partial responses (PRs; overall response rate, 63%; 95% confidence interval, 46% to 81%). The response rates in patients with and without prior chemotherapy were 63% and 64%, respectively. The median response duration was 37 weeks from start of treatment, with a maximum response duration of greater than 70 weeks. Median survival has not yet been reached. Leukopenia was the most frequently encountered toxicity, with a World Health Organization (WHO) grade greater than 3 occurring in 74% of courses. Thrombocytopenia and anemia were negligible. Only 10 patients (32%) had alopecia severe enough to wear a wig. There were no cardiac symptoms or events in any patient, but a slight median fall in left ventricular ejection fraction (LVEF) of 6% (from +7 to -12) during stress and 6% (from +14 to -14) at rest occurred. Other toxicities were mild, and the drug was generally well tolerated. CI941 is a very active and well-tolerated new agent in the treatment of advanced breast cancer, with neutropenia being the main toxicity.
Validity of questions used to assess chronic cough in epidemiological surveys
Authors: Jennifer Baeuml, Maja Jurca, Aniña Pescatore, Ben Spycher, Myrofora Goutaki, Cristian Dogaru, Philipp Latzin, Erol Gaillard, Claudia Kuehni
Journal: European Respiratory Journal (2013)

Background : A number of questions have been used to assess chronic cough in epidemiological surveys of children with and without wheeze. The comparative validity of these questions is unknown. In a population-based cohort study, we determined the association of different cough questions with current wheeze.   Methods : In a cohort study of unselected children, we assessed prevalence of respiratory symptoms repeatedly by questionnaires, including various questions on chronic cough. Using data collected in 2010, at age 13-17 years, we determined the strength of association of different cough questions with current wheeze using a logistic regression.   Results : Of 2542 participants, 359 reported current wheeze, 1621 had never wheezed and 784 had wheezed earlier but not in the last 12 months. Adjusting for age, sex and ethnicity, the following cough questions were most strongly associated with current wheeze (Odds ratio, OR>5): child coughs more than others (OR 12.1 95% CI [8.8-16.7]), cough lasts >2months (6.04; [3.2; 11.6]), phlegm at any time of day or night (6.5 ; 5.0; 8.4); frequent colds (23.1 [9.2; 57.8]), extended duration of colds (4.36 [1.2; 15.7]), cough triggered by exercise (8.0; [6.1; 10.3]) and by pets (7.7 [4.7; 12.7]). Less strongly associated were: night cough (ISAAC question; 4.9; [3.8; 6.4], cough apart from colds (3.4 [2.6; 4.4]).   Conclusion : Questions on chronic cough commonly used in respiratory questionnaires vary greatly in their association with current wheeze. This demonstrates the need to determine the comparative validity and usefulness of these questions in epidemiological surveys.   Funding :  SNF PDFMP3 137033; SNF 32003B-144068; Asthma UK 07/048.
Ventilatory responses to chemosensory stimuli in quadriplegic subjects
Authors: M Pokorski, T Morikawa, S Takaishi, A Masuda, B Ahn, Y Honda
Journal: European Respiratory Journal (1990)

We tested the hypothesis that interruption of motor traffic running down the spinal cord to respiratory muscle motoneurons suppresses the ventilatory response to increased chemical drive. We compared the hypoxic (HVR) and hypercapnic (HCVR) ventilatory responses, based on the rebreathing technique, before and during inspiratory flow-resistive loading in 17 quadriplegic patients with low cervical spinal cord transection and in 17 normal subjects. The ventilatory response was evaluated from minute ventilation (VE) and mouth occlusion pressure (P0.2) slopes on arterial oxygen saturation (SaO2) or on end-tidal PCO2 (PACO2), and from absolute VE values at SaO2 80% or at PACO2 55 mmHg. We found no difference in the unloaded HVR or HCVR between the quadriplegic and normal subjects. In the loaded HVR, the delta VE/delta SaO2 slope tended to decrease similarly in both groups of subjects. The delta P0.2/delta SaO2 slope was shifted upwards in normal subjects, yielding a significantly higher P0.2 at a given SaO2. In contrast, this rise in the P0.2 level during loaded HVR was absent in quadriplegics. Loaded HCVR yielded qualitatively similar results in both groups of subjects; delta VE/delta PACO2 decreased and delta P0.2/delta PACO2 increased significantly. The results show that the ventilatory chemosensory responses were unsuppressed in quadriplegics, although they displayed a disturbance in load-compensation, as reflected by occlusion pressure, in hypoxia. We conclude that the descending drive to respiratory muscle motoneurons is not germane to the operation of the chemosensory reflexes.