tbuckley/cabot-search huggingface dataset:
Abstract 410: Epigallocatechin gallate inhibits colon cancer cell proliferation by modulating epigenetic enzymes (DNMTs, HDACs, and HATs)
Authors: April B. Cabang, Yuan Fang, Jay Morris, Michael J. Wargovich
Journal: Cancer Research (2014)

Abstract Epigallocatechin gallate (EGCG), a major constituent of green tea, has been shown to exhibit antioxidant and antitumorigenic properties using in vitro and in vivo models of colon cancer and in small cohort studies of colorectal cancer patients. The development of colon cancer is influenced by both genetic and epigenetic factors, with increased risk associated with age, lifestyle, and environmental exposures. Thus, parsing the interactions of genetic components and epigenetic modulators of gene expression in colon cancer development and progression is essential. The mechanism by which EGCG exerts antitumorigenic activity against colon cancer has not been completely elucidated. This study investigates potential mechanism(s) by which EGCG inhibits proliferation of HCT-116 and HT-29 colon cancer cell lines, via epigenetic pathways. Briefly, HCT-116 and HT-29 cells were treated with EGCG (5-150 µM) and control drugs: 5-FU (50-200 µg/mL), 5-AZA (5 µM), SAHA (1-10 µM), and anacardic acid (10 µM) for 48-72 h. Cell proliferation (MTT), cell cycle and apoptosis (PI-FC and TUNEL), enzyme activity, gene and protein expression were measured. EGCG inhibited proliferation of HCT-116 and HT-29 by promoting S-phase cell cycle arrest and apoptosis. Expression and global enzyme activity of DNMTs (1, 3a, 3b), HDACs (1-4), and HATs (P300, PCAF, P400) were differentially expressed in both cell lines. A consistent upregulation of DNMT3b and downregulation of HDAC3 in both cell lines were observed. Global and gene-specific regulation of DNA methylation and histone acetylation by EGCG is under current study in our lab. The antiproliferative effects of EGCG can potentially be attributed to restoring normal global and gene-specific methylation/acetylation pattern in colon cancer cells by regulating DNMTs, HDACs, and HATs. This study provides evidence for the utilization of EGCG as a potential chemopreventive agent against colon cancer. Furthermore, identifying mechanisms by which EGCG overrides tumor-driven genetic deregulation to inhibit carcinogenesis expands our knowledge on pathways contributing to colon cancer and plausible targets for epigenetic therapy/prevention. This project is supported by NIH: RO1CA96694 and P30CA054174. Citation Format: April B. Cabang, Yuan Fang, Jay Morris, Michael J. Wargovich. Epigallocatechin gallate inhibits colon cancer cell proliferation by modulating epigenetic enzymes (DNMTs, HDACs, and HATs). [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 410. doi:10.1158/1538-7445.AM2014-410
Abstract 65: <i>Ink4a/Arf</i> locus drives gastric tumorigenesis through induction of parietal cell loss
Authors: Ryo Seishima, Takeyuki Wada, Hirotoshi Hasegawa, Yoshiyuki Ishii, Koji Okabayashi, Masashi Tsuruta, Yuko Kitagawa, Hideyuki Saya, Osamu Nagano
Journal: Cancer Research (2014)

Abstract Background: Cancer development is often preceded by the appearance of preneoplastic lesions. In gastric carcinogenesis, chronic inflammation and histopathologic progression of the stomach epithelium lead to the development of metaplasia and eventually adenocarcinoma. Spasmolytic polypeptide-expressing metaplasia (SPEM) is one of the preneoplastic lesions in the stomach and is triggered by the loss of parietal cells that secrete gastric acid. The Ink4a/Arf locus encodes two structurally unrelated tumor suppressor proteins, p16(INK4a) and p19(ARF) in mice. However, little is known regarding the role of these proteins encoded by the INK4a/ARF locus in gastric tumorigenesis.Methods: To evaluate the functional role of Ink4a/Arf locus, we crossed mouse gastric cancer modelK19-Wnt1/C2mE mice with Ink4a/Arf (-/-) mouse and generated Ink4a/Arf (-/-) K19-Wnt1/C2mE mice and evaluated the effect of deletion of Ink4a/Arf locus on the parietal cell loss and formation of SPEM and gastric tumor. Results: In K19-Wnt1/C2mE mice, accumulation of reactive oxygen species (ROS) and the increase of p19 expression were observed in gastric epithelium along with the parietal cells loss leading to SPEM development. However, the parietal cells loss followed by formation of SPEM and gastric tumor were drastically suppressed in Ink4a/Arf (-/-) K19-Wnt1/C2mE mice. Furthermore, treatment of tamoxifen, an inducer of parietal cells loss, in Ink4a/Arf (-/-) mice revealed that deletion of Ink4a/Arf locus suppresses parietal cells loss in mice. Conclusion: These results suggest that activation of p19(ARF) tumor suppressor protein by ROS accumulation paradoxically play a key role in the parietal cells loss leading to gastric tumorigenesis. Citation Format: Ryo Seishima, Takeyuki Wada, Hirotoshi Hasegawa, Yoshiyuki Ishii, Koji Okabayashi, Masashi Tsuruta, Yuko Kitagawa, Hideyuki Saya, Osamu Nagano. Ink4a/Arf locus drives gastric tumorigenesis through induction of parietal cell loss. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 65. doi:10.1158/1538-7445.AM2014-65
Abstract 4488: Multi-functional, miR-205 complexed, gemcitabine conjugated nanomedicines for effective treatment of chemo-resistant pancreatic adenocarcinoma
Authors: Deepak Chitkara, Anupama Mittal, Stephen W. Behrman, Ram I. Mahato
Journal: Cancer Research (2014)

Abstract Aberrant expression of small, non-coding RNAs called miRNAs in tumor tissues result in anomalous levels of genes and proteins which trigger metastasis and chemoresistance, and aggravate stemness among the cancer cells. We investigated one such miRNA, miR-205 identified through various softwares (TARGETSCAN, miRDB and DIANACOT) and validated experimentally as a potential target for treatment of advanced stage pancreatic cancer. Most of the current attempts to deliver such potent biologically complex molecules fail clinically owing to the challenges of structural and functional stability involved in their formulation and in vivo delivery. In this study, we constructed a multifunctional, self-assembling, copolymer with carboxyl pendant groups (mPEG-PCC) which exhibited efficient conjugation with gemcitabine (12.1% w/w) and simultaneously formed stable complexes with miR-205 at N/P 16/1. Both the therapeutic molecules which are otherwise known for plasma degradation demonstrated long-term stability in plasma when delivered through our system. Nanomedicines prepared using this polymer by film hydration method showed a size of ∼72.9 nm and sustained gemcitabine release of ∼40% in 10 days which was not affected by the co-presence of miRNA in the formulation. In gemcitabine resistant MIA PaCa-2R pancreatic cancer cells, the formulations showed a transfection efficiency of more than 90% (by flow cytometry), reversal of chemo-resistance (cell viability assay) and decrease in invasive and migratory ability of the cancer cells (Matrigel® invasion assays). In vivo evaluation in xenograft model developed using MIA PaCa-2R cells indicated significantly diminished tumor growth in miR-205/gemcitabine nanomedicines treated group. Note: This abstract was not presented at the meeting. Citation Format: Deepak Chitkara, Anupama Mittal, Stephen W. Behrman, Ram I. Mahato. Multi-functional, miR-205 complexed, gemcitabine conjugated nanomedicines for effective treatment of chemo-resistant pancreatic adenocarcinoma. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4488. doi:10.1158/1538-7445.AM2014-4488
976. Decreased Tumorigenic Potential of EphA2-Overexpressing Breast Cancer Cells Following Treatment with Adenoviral Vectors That Express EphrinA1
Authors: Loren W. Noblitt, Dinesh S. Bangari, Shruti Shukla, Deborah W. Knapp, Sulma Mohammed, Michael S. Kinch, Suresh K. Mittal
Journal: Molecular Therapy (2004)

The EphA2 receptor tyrosine kinase is frequently overexpressed in invasive breast cancer cells. Moreover, these malignant cells have unstable cell-cell contacts, which preclude EphA2 from interacting with its ligand, EphrinA1, which is anchored to the membrane of adjacent cells. This defect is important because ligand binding causes EphA2 to transmit signals that negatively regulate tumor cell growth and survival, whereas the absence of ligand binding favors these same behaviors. In our present study, human adenoviral type 5 (HAd) vectors were engineered to express secreted-forms of EphrinA1. These vectors were used to infect MDA-MB-231 human breast cancer cells, or MCF-10A human breast epithelial cells providing matched controls. Infection with HAd-EphrinA1-Fc (HAd vector expressing extracellular domain of human EphrinA1 attached to Fc portion of human IgG1 heavy chain) caused increased EphA2 activation and turnover and consequently decreased tumor cell viability in suspension culture. Consistent with this observation, infection of MDA-MB-231 cells with HAd-EphrinA1-Fc prevented tumor formation in xenograft models. Furthermore, therapeutic modeling via intratumoral inoculation revealed that HAd-EphrinA1-Fc significantly inhibited subsequent tumor growth as compared to matched controls. These results suggest that targeting of EphA2 with adenoviral vectors may have therapeutic value.
Conjugated Equine Estrogens Inhibit Progression of Atherosclerosis but Have No Effect on Intimal Hyperplasia or Arterial Remodeling Induced by Balloon Catheter Injury in Monkeys 11This work was supported in part by Grant PO1-HL45666 from the National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland. Wyeth-Ayerst, Princeton, New Jersey provided the Premarin used in this study.
Authors: Randolph L Geary, Michael R Adams, Marshall E Benjamin, J.Koudy Williams
Journal: Journal of the American College of Cardiology (1998)
Developmental Trajectories of Physical Activity, Sports, and Television Viewing During Childhood to Young Adulthood
Authors: Soyang Kwon, Kathleen F. Janz, Elena M. Letuchy, Trudy L. Burns, Steven M. Levy
Journal: JAMA Pediatrics (2015)

The diverse developmental patterns of obesogenic behaviors during childhood and adolescence can be better understood by using new analytic approaches to assess the heterogeneity in variation during growth and development and to map the clustering of behavior patterns.
Abstract P371: Healthy Clinics, Healthy Kids - Lifestyle Intervention on Cardiometabolic Disease Prevention in Pediatric Clinical Settings
Authors: Danielle Hollar, Caitlin Heitz, Michelle Lombardo
Journal: Circulation (2014)

Childhood obesity should be addressed in multiple sectors, including the health services/clinical setting. While many physicians believe it is their responsibility to help adult patients lose weight, the majority feels they are not equipped to manage weight problems of their patients. Healthy Clinics, Healthy Kids (HCHK) was designed to test the impact of addressing obesity, using a lifestyle approach, among pediatric patients and families in a variety of primary care clinical settings. HCHK interventions took place over a 2-year period (2010-2012) in three states (MI, MS and NM). Lifestyle interventions (nutrition, healthy living, physical activity education/technical assistance), targeted children and caregivers in low-income clinical settings that care for children. The goal was to help clinical staff address obesity issues and concerns of patients and families. Impact of interventions was assessed using pre and post surveys. 21 sites completed pre and post surveys (MI (9), MS (9), and NM (3)). Respondents included nurses (14), managers (2), physicians (2), coordinators (1), and a registered dietitian. Prevalence of obesity among children was higher than the national average (respondents reported that only 56% were “normal”, as compared to approximately 66% nationally). 32% indicated private insurance pays for some obesity care, and 42% indicated public insurance. 44% indicated they discuss weight with parents of “normal” weight children, and about 73% indicated they discuss this with parents of “overweight” children. Statistically significantly (at the p&lt;.05 level) more respondents indicated, at the end of the project than at the beginning, that they discuss “the impact of childhood obesity on their future risk for chronic diseases in adulthood” (70% versus 57%). Statistically significantly more indicated that they discuss this topic with parents at the end (76% versus 57%). There was an increase in discussing obesity with patients, topics including exercise and physical activity (+8.5%) and limiting sedentary behaviors (+5.0%). There was an increase in conversations with parents, topics including healthy food and beverage choices (+4.8%), physical activity (+9.4%), limiting sedentary behaviors (+4.7%), “creating a healthy home environment” (+19.1%), and “removing the TV from the bedroom” (+14.3%) Although at baseline it was not discussed much (22.2%), at follow-up, there was an increase in “encouraging breastfeeding for childhood obesity prevention, if pregnant” (38.1%). Regarding parents, although already a topic with high comfort level at baseline, statistically significantly more respondents indicated at the end of the project that they felt comfortable discussing exercise and physical activity (76.2% versus 90.5% respectively). Overall, HCHK showed that implementation of a nutrition and healthy living program for clinics is feasible and effective.
Controversy in managing patients with prostate cancer
Authors: A. G Mulley, M. J Barry
Journal: BMJ (1998)

Life is uncertain, and never more so than when a serious illness like prostate cancer strikes and a decision must be made about how to proceed. Ideally, the clinician would find (or remember) the relevant research, interpret the findings as they apply to the patient at hand, estimate prognosis, and discuss treatment options objectively and with compassion and support. Even then, life remains uncertain; for any particular patient, no matter how good the evidence and precise the probability estimates, there can be no guarantee.

This irreducible uncertainty is confronted routinely by doctors and patients and is rarely a source of clinical controversy. Trouble begins when experts reach different conclusions from the same piece of evidence. The poorer the evidence, the more discretionary the interpretation, and the more controversial the conclusion. When available evidence is totally inadequate to inform decisions that must be made, then clinical controversy may border on chaos. Savage and others have shown that in Britain and elsewhere controversy and chaos reign in the management of prostate cancer.1-3

For early prostate cancer, there have been no controlled trials good enough to show whether survival is increased by active intervention with radical prostatectomy, radiation, or hormonal therapy. For men …
Mater Dolorosa (Sorrowing Madonna)
Authors: M. Therese Southgate
Journal: JAMA (1999)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
End-of-Life Practices in European Intensive Care UnitsEnd-of-Life Practices in European Intensive Care Units
Authors: Alberto Giannini
Journal: JAMA (2003)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Lead Exposure and Cataract Risk in Men—Reply
Authors: Debra A. Schaumberg, Howard Hu
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 15309: Impaired Gas Diffusion and RV to Pulmonary Circulation Uncoupling Limit Exercise Performance in Heart Failure Patients
Authors: Valentina Labate, Francesco Bandera, Greta Generati, Marta Pellegrino, Eleonora Alfonzetti, Marco Guazzi
Journal: Circulation (2014)

Introduction: In heart failure (HF) patients, an altered gasdiffusing capacity for carbon monoxide (DLCO) is a marker of lung capillary injury that bears relevant clinical and prognostic information. It is unknown whether right heart-pulmonary circulation (RH-PC) uncoupling abnormalities and gas diffusion are linked and may become synergic in causing exercise limitation and ventilation inefficiency.

Methods: 17 HF patients (mean age 64±11; male 75%; NYHA II-III; mean left ventricular (LV) ejection fraction 34±9%) underwent DLCO measurements with assessment of membrane component (DM) an capillary blood volume (Vc) and underwent to maximal cardiopulmonary exercise testing (CPET, tilt-ergometer,personalized ramp protocol) combined with Echo-Doppler assessment of right ventricular function by assessing tricuspid annular peak systolic excursion (TAPSE) and pulmonary systolic pressure (PASP).

Results: Patients exhibited an abnormal gas diffusion (mean DLCO 17±3.9 ml/min/mmHg) with depressed alveolar-capillary membrane diffusing capacity (DM) component (mean 23.4±6.8 ml/min/mmHg) and elevated capillary volume (mean 111.2±64 ml) along with significant functional limitation (mean peak VO2 12.5±3.7 ml/kg/min) and ventilatory inefficiency(mean VE/VCO2 slope: 34.4±6.9 and mean end-tidal of CO2 mean 32.1±5.2 mmHg). Significant correlations were found between DM, TAPSE/PASP relationship, peak VO2 and VE/VCO2 slope at peak exercise (figure).

Conclusions: Our findings show a link between the RV-PC uncoupling with gas diffusion abnormalities suggesting that interventions aimed at targeting the functional performance in HF population have to ideally combine amodulatory effect on both the alveolar-capillary gas diffusion capacity and right heart function.

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Peripheral Retinopathy in Offspring of Carriers of Norrie Disease Gene Mutations
Authors: Helen A. Mintz-Hittner, Robert E. Ferrell, Katherine B. Sims, Kristina M. Fernandez, Billie S. Gemmell, Dale R. Satriano, Jon Caster, Frank L. Kretzer
Journal: Ophthalmology (1996)
Variation in NRT1.1B contributes to nitrate-use divergence between rice subspecies
Authors: Bin Hu, Wei Wang, Shujun Ou, Jiuyou Tang, Hua Li, Ronghui Che, Zhihua Zhang, Xuyang Chai, Hongru Wang, Yiqin Wang, Chengzhen Liang, Linchuan Liu, Zhongze Piao, Qiyun Deng, Kun Deng, Chi Xu, Yan Liang, Lianhe Zhang, Legong Li, Chengcai Chu
Journal: Nature Genetics (2015)
A Swarm of Shadow
Authors: Jessica Maich
Journal: JAMA (2001)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal