tbuckley/cabot-search huggingface dataset:
Identification of an Association Between Genomic Hypomethylation of <i>FCGR2A</i> and Susceptibility to Kawasaki Disease and Intravenous Immunoglobulin Resistance by DNA Methylation Array
Authors: Ho‐Chang Kuo, Jen‐Chieh Chang, Hsing‐Chun Kuo, Hong‐Ren Yu, Chih‐Lu Wang, Chiu‐Ping Lee, Li‐Tong Huang, Kuender D. Yang
Journal: Arthritis & Rheumatology (2014)

Objective Kawasaki disease (KD) is characterized by systemic vasculitis, and it is the most common acquired heart disease in children. However, the etiology and immunopathogenesis of KD are still unclear. A genome‐wide association study (GWAS) identified polymorphisms in CD40 , BLK , and FCGR2A as the susceptibility genes for KD. No epigenetic array studies of KD have previously been published. This study was undertaken to investigate differences in DNA methylation in patients with KD as compared to controls. Methods The HumanMethylation27 BeadChip (Illumina) was used to survey the differences in DNA methylation between KD patients and controls. DNA methylation array validation was performed in a separate cohort by pyrosequencing assay and reporter gene assays. Messenger RNA (mRNA) expression was determined, and the association of methylation with response to intravenous immunoglobulin (IVIG) treatment was analyzed. Results HumanMethylation27 BeadChip assay showed a 15% difference in methylation of 10 genes between KD patients and controls. The FCGR2A cg24422489 group, which was recently reported to be associated with KD susceptibility in a GWAS, had significant hypomethylation of 15.54% less in the KD group than in the control group. Validation of FCGR2A methylation in another cohort also showed significant hypomethylation in the KD group (5 of 5 CpG sites [ P &lt; 0.01]; n = 43 in the KD group and n = 55 in the control group). KD patients with IVIG resistance showed hypomethylation of 5 CpG sites ( P &lt; 0.05). FCGR2A mRNA expression was significantly increased in patients in the acute stage of KD compared to controls. Reporter gene assays indicated that the CpG sites of the FCGR2A promoter region were sufficient to modulate gene expression. Conclusion This is the first study to examine the DNA methylation array in KD and identify a role of hypomethylation of FCGR2A in susceptibility to KD and IVIG resistance.
Ezetimibe Added to Statin Therapy after Acute Coronary Syndromes
Authors: Christopher P. Cannon, Michael A. Blazing, Robert P. Giugliano, Amy McCagg, Jennifer A. White, Pierre Theroux, Harald Darius, Basil S. Lewis, Ton Oude Ophuis, J. Wouter Jukema, Gaetano M. De Ferrari, Witold Ruzyllo, Paul De Lucca, KyungAh Im, Erin A. Bohula, Craig Reist, Stephen D. Wiviott, Andrew M. Tershakovec, Thomas A. Musliner, Eugene Braunwald, Robert M. Califf
Journal: New England Journal of Medicine (2015)

Statin therapy reduces low-density lipoprotein (LDL) cholesterol levels and the risk of cardiovascular events, but whether the addition of ezetimibe, a nonstatin drug that reduces intestinal cholesterol absorption, can reduce the rate of cardiovascular events further is not known.
Oxidant and antioxidant-induced perturbation in redox metabolism and apoptosis in PC12 cells
Authors: haider raza, Annie John, Eric Mensa-Brown, Sheela Benedict, Amr Kambal
Journal: Cancer Research (2007)

4584   Cellular oxidative stress and alterations in redox metabolisms have been implicated in the etiology and pathology of cancer. The sensitivity and resistance of cancer cells towards chemotherapeutic drugs have also been shown to be dependent on the cellular redox status. Induction of apoptosis and inhibition of cellular proliferation are therefore logical strategies to control and prevent cancer development. We have previously shown marked alterations in the oxidative stress in PC12 cells after treatment with the oxidant 4-hydroxynonenal (HNE) and the antioxidant epigallocatechin gallate. In the present study, we further demonstrate the role of HNE and natural antioxidants, curcumin and melatonin on redox metabolisms, mitochondrial functions and apoptosis in PC12 cells. Cellular oxidative stress induced after HNE treatment was accompanied by the increased expressions of GSTA4-4, Hsp70, CYP2E1 and nuclear NFkB activation. The reduced glutathione (GSH) pool and mitochondrial oxygen metabolism by cytochrome c oxidase appear to be the major reasons for the increased oxidative stress. Apoptosis, as measured by flow cytometry, TUNEL and DNA laddering assays, was markedly induced after HNE treatment. Curcumin and melatonin treatments, on the other hand, maintained cellular GSH pool without activation of apoptosis in these cells. A decrease in the nuclear translocation of NFkB (p65) was also seen after curcumin and melatonin treatments. These results suggest that altered redox metabolism and mitochondrial respiratory functions may be critical in cancer cell survival and death.  (Supported by Terry Fox Cancer Research Funds, UAE).
Abstract 13511: Cardiac Release Kinetics of Soluble Fms-Like Tyrosine Kinase 1 (sflt-1) And Hstroponin T Identify Sflt-1 as an Early Marker of Myocardial Ischemia in Acs
Authors: Salvatore De Rosa, Stephan Fichtlscherer, Ralf Lehmann, Tino Roexe, Marga Mueller-Ardogan, Stefanie Dimmeler, Andreas M Zeiher
Journal: Circulation (2011)
The additive burden of iron deficiency in the cardiorenal–anaemia axis: scope of a problem and its consequences
Authors: IJsbrand T. Klip, Ewa A. Jankowska, Cristina Enjuanes, Adriaan A. Voors, Waldemar Banasiak, Jordi Bruguera, Piotr Rozentryt, Lech Polonski, Dirk J. van Veldhuisen, Piotr Ponikowski, Josep Comin‐Colet, Peter van der Meer
Journal: European Journal of Heart Failure (2014)
Is cryoablation really more effective than radiofrequency ablation in hepatocellular carcinoma?
Authors: Gin‐Ho Lo
Journal: Hepatology (2015)

Potential conflict of interest: Nothing to report. To the Editor: I read with interest the article by Wang et al.1 regarding the comparison between cryoablation and radiofrequency ablation (RFA) in the management of small hepatocellular carcinoma (HCC). The authors demonstrated that cryoablation had lower local tumor progression than RFA. We should applaud the authors' execution of a well‐designed randomized controlled trial in the management of small HCC; however, several concerns should be addressed. All of the patients enrolled in the study had cirrhosis, and approximately 90% of the cases were related to hepatitis B virus. Based on the Asian‐Pacific consensus guidelines,2 antiviral therapy in patients with cirrhosis and/or HCC and hepatitis B viral DNA >2000 IU/mL can prevent decompensation and potentially tertiary prevention of HCC recurrence. This important issue regarding the use of antiviral therapy in the cryoablation and RFA groups was not disclosed in this study. It would be unethical to let suitable candidates for antiviral therapy go untreated and unfair for patients in the RFA group, a greater number of whom belonged to Child‐Pugh class B. Moreover, five patients in the RFA group did not undergo another course of RFA simply due to tumors of 4 cm. This appeared to be unjustified because a tumor ≤4 cm was considered an indication for ablation therapy in this trial. Some of these patients received percutaneous ethanol injection treatment alone. It was apparently not suitable for these patients with liver tumor size of 4 cm to receive percutaneous ethanol injection only.3 This approach likely influenced the local tumor progression rate in the RFA group.
Knocking down catalase leads to increased ROS production and proliferation in glioma cells
Authors: Pameeka S. Smith, Weiling Zhao, Mike Robbins
Journal: Cancer Research (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

4417 

Gliomas are resistant to cancer therapies resulting in poor patient survival. We hypothesize that this resistance is due, in part, to an altered antioxidant enzyme profile. Catalase, a primary antioxidant enzyme that scavenges H2O2, is constitutively elevated in gliomas when compared to their normal cell counterpart, the astrocyte. Catalase enzymatic activity is 2-fold higher and greater than 4-fold higher in C6 and 36B10 rat glioma cell lines, respectively. Furthermore, catalase protein and activity are elevated in numerous other rat and human glioma cell lines. Increasing evidence supports a functional role for reactive oxygen species (ROS) in signaling cascades that promote proliferation, differentiation, and cell death. We hypothesize that knockdown of catalase in glioma cells would increase ROS production leading to an increased sensitivity to anticancer therapies mediated via oxidative stress. To test this hypothesis, we have implemented two approaches to knock-down catalase expression in C6 and 36B10 cell lines. The first, a pharmacological approach, uses 3-amino 1,2,4 triazole (3-AT), an irreversible inhibitor of catalase. Incubation of glioma cells with 1-10 mM 3-AT led to a dose-dependent reduction in catalase activity; 10 mM 3-AT completely abrogated catalase enzymatic activity in C6 gliomas; 100 mM 3-AT was required to inhibit catalase activity in 36B10 cells due to higher constitutive catalase activity. Additionally, we have generated glioma cells stably expressing catalase shRNA using the Ambion® p Silencer ™ 4.1-CMV puro expression vector. These stables show a 75% reduction in catalase protein accompanied with a corresponding reduction in catalase activity. Unexpectedly, catalase inhibition via pharmacological and molecular approaches failed to radiosensitize gliomas cells. Of interest, glioma cell lines stably expressing catalase shRNA demonstrated an elevation in intracellular ROS accompanied by increased cell viability as assessed by MTT. Moreover, inhibition of catalase was not compensated by an increase in glutathione peroxidase (GPX) activity. Further analyses show that cells stably expressing catalase shRNA exhibit an increase in cell proliferation when compared to controls. We hypothesize that the ROS generated via catalase inhibition are utilized to signal growth and survival rather than cell death. Therefore, we propose that these stable glioma cell lines represent a novel tool to define the role of peroxides in glioma radioresistance and proliferation.
Long-term Cancer Control Outcomes in Patients with Clinically High-risk Prostate Cancer Treated with Robot-assisted Radical Prostatectomy: Results from a Multi-institutional Study of 1100 Patients
Authors: Firas Abdollah, Akshay Sood, Jesse D. Sammon, Linda Hsu, Burkhard Beyer, Marco Moschini, Giorgio Gandaglia, Craig G. Rogers, Alexander Haese, Francesco Montorsi, Markus Graefen, Alberto Briganti, Mani Menon
Journal: European Urology (2015)
Abstract 58: Age-specific Exercise Capacity Threshold for All-cause Mortality Risk in Men
Authors: Peter Kokkinos, Jonathan Myers, Charles Faselis, Xuemei Sui, Jiajia Zhang, Steven Blair
Journal: Circulation (2014)

Introduction: Exercise capacity is inversely associated with mortality risk. However, there is no age-specific standardized exercise capacity threshold to guide clinical assessment of mortality risk. Hypothesis: We assessed the hypothesis that an age-specific standardized threshold can be defined based on maximal exercise capacity (METs) to assess mortality risk across age groups. Methods: Peak exercise capacity was assessed in 18,102 men (mean age: 58±11) at DC and Palo Alto CA VA Medical Centers. We stratified the cohort into four age-groups (&lt;50; 50-59; 60-69; and ≥70 yrs of age). We applied Cox proportional hazards models with spline function of MET to define the MET level associated with no increase in mortality risk (hazard ratio (HR)=1.0). We formed five additional age-specific fitness categories based on METs achieved above and below this threshold (T1; n=3,705): T2=T1-2 METs (n=4,884); T3; T1-&gt;2 METs (n=1,692); T4=T1+2 METs (n=4,646); T5=T1+2-4 METs (n=1,874) and T6=T1+&gt;4 METs (n=1,301). Results: The MET thresholds for the aforementioned age groups were identified as 8; 7; 6; and 5 METs, respectively. There were 5,103 deaths (median follow-up=10.8 yrs; 208,108 person-yrs). Cox proportional hazards analysis adjusted for age, cardiac risk factors, medications and muscle-wasting disease revealed 19% (HR=1.19; CI: 1.10-1.28) and 46% (HR=1.46; CI:1.33-1.60) higher all-cause mortality risk for those with exercise capacity 2 and &gt;4 METs below the threshold, respectively. For those with an exercise capacity 2; 4 and &gt;4 METs above the threshold, mortality risk was lower by 27% (HR= 0.73; CI:0.66-0.79), 35% (HR= 0.65; CI: 0.58-0.74), and 48% (HR=0.52; CI:0.45-0.62), respectively (p&lt;0.001 for all comparisons). The trends for mortality risk were similar for each age category (Table). Conclusions: We identified an age-specific MET threshold for fitness-related clinical assessment of mortality risk in men.
Randomised trial of acupuncture compared with conventional massage and "sham" laser acupuncture for treatment of chronic neck pain Commentary: Controls for acupuncture---can we finally see the
Authors: D. Irnich
Journal: BMJ (2001)

<h3>Abstract</h3> <b>Objectives:</b> To compare the efficacy of acupuncture and conventional massage for the treatment of chronic neck pain. <b>Design:</b> Prospective, randomised, placebo controlled trial. <b>Setting:</b> Three outpatient departments in Germany. <b>Participants:</b> 177 patients aged 18–85 years with chronic neck pain. <b>Interventions:</b> Patients were randomly allocated to five treatments over three weeks with acupuncture (56), massage (60), or “sham” laser acupuncture (61). <b>Main outcome measures:</b> Primary outcome measure: maximum pain related to motion (visual analogue scale) irrespective of direction of movement one week after treatment. Secondary outcome measures: range of motion (3D ultrasound real time motion analyser), pain related to movement in six directions (visual analogue scale), pressure pain threshold (pressure algometer), changes of spontaneous pain, motion related pain, global complaints (seven point scale), and quality of life (SF-36). Assessments were performed before, during, and one week and three months after treatment. Patients9 beliefs in treatment were assessed. <b>Results:</b> One week after five treatments the acupuncture group showed a significantly greater improvement in motion related pain compared with massage (difference 24.22 (95% confidence interval 16.5 to 31.9), P=0.0052) but not compared with sham laser (17.28 (10.0 to 24.6), P=0.327). Differences between acupuncture and massage or sham laser were greater in the subgroup who had had pain for longer than five years (n=75) and in patients with myofascial pain syndrome (n=129). The acupuncture group had the best results in most secondary outcome measures. There were no differences in patients9 beliefs in treatment. <b>Conclusions:</b> Acupuncture is an effective short term treatment for patients with chronic neck pain, but there is only limited evidence for long term effects after five treatments. <h3>What is already known on this topic</h3> Acupuncture is a widespread complementary treatment Evidence from trials have given conflicting results on its use in the treatment of neck pain because of methodological shortcomings and because effects were compared either with alternative treatments or with different sham procedures imitating acupuncture, but not both <h3>What this study adds</h3> Compared with sham laser acupuncture and massage, needle acupuncture has beneficial effects on mobility and pain related to motion in patients with chronic neck pain Acupuncture was clearly more effective than massage, but differences were not always significant compared with sham laser acupuncture Acupuncture was the best treatment for patients with the myofascial syndrome and those who had had pain for longer than five years
Abstract 13164: Differential Expression of Myocardial Pronatriuretic Peptide Convertases Furin and Corin in Experimental Heart Failure
Authors: Tomoko Ichiki, Brenda K Huntley, Guido Boerrigter, Denise M Heublein, Gail J Harty, S. Jeson Sangaralingham, Michihisa Jougasaki, John C Burnett
Journal: Circulation (2010)

Background: Natriuretic peptides (NPs) play important roles as cardiorenal protective peptides. ProNPs are non-functional precursors of active NPs. ProANP is processed to its active form by the pro...
Oh NHS, thou art sick
Authors: R. Smith
Journal: BMJ (2002)

Papers p 135 

It seems to be universally agreed that the NHS is sick. It is plagued by delay, low quality care, and poor outcomes.1 The chancellor of the exchequer has called for a great debate on the service,2 which must include exploring the causes of the sickness and possible treatments. Ideally the debate will be informed by evidence, and the BMJ today publishes a study that is an important contribution (p 135).3 It is a broad brush comparison between the NHS and Kaiser Permanente, a health maintenance organisation that cares for some 6.1 million Californians. The study finds that the two systems have similar resources but that Kaiser performs substantially better. In particular, patients from Kaiser have faster access to both primary and secondary care doctors. In other words, the widely held idea that the NHS—albeit cheap—is remarkably efficient may be wrong.

Comparisons between systems are always difficult, and readers will rightly be sceptical, not least because they challenge a belief that is widely and deeply held in Britain. Our reviewers and editorial team started from that position but concluded that the results could not be explained by artefacts in the study or differences …
Abstract 12028: Left Ventricular Contractile Reserve Assessed by Exercise Stress Echocardiogram in Adults With Repaired Tetralogy of Fallot: A Novel Early Marker of Intrinsic Myocardial Disease?
Authors: Amelie Michaud, Pier-Anne Gilbert, Michael A Gatzoulis, Philippe Pibarot, Josep Rodés-Cabau, Jean Perron, Elisabeth Bedard
Journal: Circulation (2014)

Background and objective:  Left (LV) and right (RV) ventricular systolic dysfunction have been identified as risk factors for adverse outcomes in patients with repaired Tetralogy of Fallot (TOF) and early detection is mandatory. Using exercice stress echocardiography (ESE), this study investigated LV contractile reserve in TOF patients compared with healthy adults.   Methods:  28 adults with repaired TOF and 28 age- and sex- matched controls were propectively evaluated with supine bicycle ESE. Contractile reserve was evaluated by mesuring LV stroke volume at rest and on exertion.   Results:  TOF patients (age, 35±10 years; male, 14) had a smaller increase in LV stroke volume on maximal exertion than controls, even when corrected for maximum workload performed (12±16% vs 28±16%, p=0,015) (Figure 1). No correlation was found between exercice stroke volume and degree of pulmonary insufficiency or RV and LV size and function at rest.   Conclusions:  LV contractile reserve is impaired in patients with repaired TOF, independently of their degree of pulmonary insufficiency or RV and LV size and function. Importantly, these findings may suggest an intrinsic LV myocardial abnormality in TOF patients, for which early detection may be achieved by ESE. Larger studies are needed to determine if impaired contractile reserve represents an independent risk factor for ventricular tachycardia and or death in these patients.
Hypertension in Adults Across Age Groups—Reply
Authors: Donald M. Lloyd-Jones, Jane C. Evans, Daniel Levy
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Crisis Awaiting Heart Transplantation
Authors: Lynne Warner Stevenson
Journal: JAMA Internal Medicine (2015)

The number of heart transplants performed in the United States was 2177 in 1994 and 2166 in 2014. However, the number of transplant centers has increased, and the criteria for transplants have broadened to include patients 65 years or older, those with a body mass index greater than 30, and more comorbid conditions, such as diabetes mellitus and a history of smoking. As the transplant waiting list has become longer and waiting times have increased, the major route to heart transplants has become deterioration to the most urgent priority status, which accounts for 10% of patients on the waiting list but two-thirds of transplants. Many heart transplant candidates develop life-threatening complications of a ventricular assist device implanted to avert death while waiting. Some affluent patients, however, can afford to temporarily relocate and obtain a transplant in regions where the waiting times are shorter without prior surgery to implant a ventricular assist device. The ethics of allocating hearts for transplant have always recalled the classic lifeboat dilemma of how many people can be allowed to board an already overcrowded lifeboat without sinking the ship and everyone on board. As transplant physicians, we advocate with the best intentions on behalf of our own patients rather than denying transplants to those less likely to benefit. In recognizing our responsibilities as stewards of scarce donor hearts, we should reduce new listings for heart transplants, thus restoring balance to the waiting list and keeping the lifeboat afloat.
The Apoptosis Inhibitor Gene API2 and a Novel 18q Gene,MLT, Are Recurrently Rearranged in the t(11;18)(q21;q21) Associated With Mucosa-Associated Lymphoid Tissue Lymphomas
Authors: Judith Dierlamm, Mathijs Baens, Iwona Wlodarska, Margarita Stefanova-Ouzounova, Jesus Maria Hernandez, Dieter Kurt Hossfeld, Christiane De Wolf-Peeters, Anne Hagemeijer, Herman Van den Berghe, Peter Marynen
Journal: Blood (1999)
Resistance of Various Inbred Strains of Mice to Tumor Homoiotransplants, and Its Relation to the &lt;italic&gt;H-2&lt;/italic&gt; Allele Which Each Carries&lt;xref ref-type="fn" rid="fn1"&gt;2&lt;/xref&gt;
Authors: G. D. Snell, Elizabeth Russell, Elizabeth Fekete, Priscilla Smith
Journal: JNCI Journal of the National Cancer Institute (1953)

Journal Article Resistance of Various Inbred Strains of Mice to Tumor Homoiotransplants, and Its Relation to the H-2 Allele Which Each Carries Get access G. D. Snell, G. D. Snell Roscoe B. Jackson Memorial Laboratory, Bar Harbor, Maine Search for other works by this author on: Oxford Academic PubMed Google Scholar Elizabeth Russell, Elizabeth Russell Roscoe B. Jackson Memorial Laboratory, Bar Harbor, Maine Search for other works by this author on: Oxford Academic PubMed Google Scholar Elizabeth Fekete, Elizabeth Fekete Roscoe B. Jackson Memorial Laboratory, Bar Harbor, Maine Search for other works by this author on: Oxford Academic PubMed Google Scholar Priscilla Smith Priscilla Smith Roscoe B. Jackson Memorial Laboratory, Bar Harbor, Maine Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 14, Issue 3, December 1953, Pages 485–491, https://doi.org/10.1093/jnci/14.3.485 Published: 01 December 1953 Article history Received: 15 June 1953 Published: 01 December 1953