tbuckley/cabot-search huggingface dataset:
Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma
Authors: A. Asplund, M. Gry Björklund, C. Sundquist, S. Strömberg, K. Edlund, A. Östman, P. Nilsson, F. Pontén, J. Lundeberg
Journal: British Journal of Dermatology (2008)

Background Basal cell carcinomas (BCCs) are prevalent tumours with uniform histology that develop without any known precursor lesion. Alterations in the sonic hedgehog–patched1 signalling pathway are accepted as necessary events for tumorigenesis, and mutations in the patched1 gene are frequently present in tumours. Objectives To analyse transcript profiles in BCC. Methods We used laser‐assisted microdissection to isolate and collect cell populations defined under the microscope. Peripheral cells from nests of BCC were selected to represent tumour cells, and normal keratinocytes from epidermis basal layer were used as control. Extracted RNA was amplified and hybridized on to a cDNA microarray. Results Our results show that BCC cells express a transcript signature that is significantly different from that of normal keratinocytes, and over 350 genes with various functions were identified as differentially expressed. The compiled data suggest an upregulation of the Wnt signalling pathway as a major event in BCC cells. Furthermore, tumour cells appear to have an increased sensitivity to oxygen radicals and dysregulated genes involved in antigen presentation. Results were validated at both the transcriptional level using real‐time polymerase chain reaction and at the protein level using immunohistochemistry. Conclusions We show that microdissection in combination with robust strategies for RNA extraction, amplification and cDNA microarray analysis allow for reliable transcript profiling and that antibody‐based proteomics provides an advantageous strategy for the analysis of corresponding differentially expressed proteins. We found that expression patterns were significantly altered in BCC cells compared with basal keratinocytes and that the Wnt signalling pathway was upregulated in tumour cells.
Subcommittee on Antifungal Susceptibility Testing of the ESCMID European Committee for Antimicrobial Susceptibility Testing. EUCAST Technical Note on the method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for conidia-forming moulds.
Authors: JL Rodriguez-Tudela, JP Donnelly, MC Arendrup, S Arikan, F Barchiesi, J Bille, E Chryssanthou, M Cuenca-Estrella, E Dannaoui, D Denning, W Fegeler, P Gaustad, C Lass-Floerl, C Moore, M Richardson, A Schmalreck, A Velegraki, P Verweij
Journal: Clinical Microbiology and Infection (2009)
Hungary tries to increase organ donation
Authors: Carl Kovac
Journal: BMJ (2001)

Hungary's health ministry has drafted a document to establish a non-profit public service company to coordinate the activities of the national organ donor service and organise the distribution of organs suitable for transplant.

The new body, Hungarotransplant Kht, will maintain a register of patients awaiting new organs and join the Eurotransplant organisation, which coordinates the search for and registration of donor hearts, livers, and kidneys in Germany, Belgium, …
Abstract 1279: “Dysfunctional” High-density Lipoprotein Despite Normal Cholesterol Content in Sedentary Obese Women
Authors: Edward Vazquez, Amar A Sethi, Maureen Sampson, Alan T Remaley, Hira Chaudhry, Richard O Cannon
Journal: Circulation (2009)

Background Clinical trials support the belief that high-density lipoprotein (HDL) is atheroprotective. However, recent studies suggest that some patients with, or at risk for, atherosclerosis may h...
Presence of autoimmune regulator and absence of desmoglein 1 in a thymoma in a patient with pemphigus foliaceus
Authors: A. Tsuchisaka, S. Kaneko, K. Imaoka, M. Ota, K. Kishimoto, U. Tomaru, M. Kasahara, C. Ohata, M. Furumura, S. Takamori, E. Morita, T. Hashimoto
Journal: British Journal of Dermatology (2014)

Funding sources: This study was supported by Grants‐in‐Aid for Scientific Research (Nos 20390308, 20591331, 21659271, 23591634, 23791298, 23791299, 23791300, 23791301, 24659534, 24591672, 24591640 and 24791185); Supported Program for the Strategic Research Foundation at Private Universities 2009‐2013 from the Ministry of Education, Culture, Sports, Science and Technology; and by the ‘Research on Measures for Intractable Diseases’ project, matching fund subsidy (H23‐028 to K.I. and H24‐038 to T.H.), from the Ministry of Health, Labour and Welfare. The study was also supported by grants from the Kaibara Morikazu Medical Science Promotion Foundation, Ishibashi Foundation, Kanae Foundation for the Promotion of Medical Science, Takeda Science Foundation, Chuo Mitsui Trust and Banking Company, Limited, and Nakatomi Foundation. Conflicts of interest: none declared. Dear Editor, Desmoglein (Dsg)1 and Dsg3 are autoantigens for pemphigus foliaceus (PF) and pemphigus vulgaris (PV), respectively.1 2 Thymomas are frequently associated with various autoimmune diseases, particularly myasthenia gravis (MG).3 Thymomas are divided histopathologically into types A, AB, B1, B2, B3 and C (renamed thymic carcinoma).4
Abstract 10132: Integration of microRNA Expression Profiling and a Validated Gene Signature from Peripheral Blood Mononuclear Cells in Pulmonary Hypertension Associated with Sickle Cell Disease
Authors: Ankit A Desai, Tong Zhou, Homaa Ahmad, Sharon Trevino, Michael Wade, Tejas Thiruvoipati, Kristin Turner, Nicole Artz, Amit R Patel, Roberto M Lang, Joe G Garcia, Roberto F Machado
Journal: Circulation (2011)

Ankit A Desai, 2011 Finalist and Presenting Author
Spatial and temporal expression of basic fibroblast growth factor protein during wound healing of rat skin
Authors: Y. Kibe, H. Takenaka, S. Kishimoto
Journal: British Journal of Dermatology (2000)

Basic fibroblast growth factor (bFGF) stimulates the mitogenesis of various cells and plays a key part in wound healing.To determine the spatial and temporal expression of bFGF protein during wound healing after burning of rat skin.Immunohistochemical methods were used.The immunostaining for bFGF in the normal epidermis was faint and sporadic in the basal cell layer. However, significant staining for bFGF was found in four locations: regenerated epidermis, a band-like zone near the regenerated epidermis, renewed capillaries, and cells infiltrating into the granulation tissue at the inflammatory to proliferative stages after the burn. The intensity of immunostaining of regenerated epidermis, the band-like zone and renewed capillaries was maximal during the proliferative stage and decreased to normal levels or disappeared simultaneously with wound closure. Immunopositive macrophage-like cell numbers in the granulation tissue increased during the proliferative stage and promptly decreased after wound closure, but such cells were only poorly visible in the scar tissue until 42 days postburn.bFGF may affect the proliferation, differentiation and migration of regenerated keratinocytes and the recruitment of inflammatory cells, as well as neovascularization in granulation tissue during wound healing. Macrophages may play a pivotal role in cutaneous wound repair by producing bFGF not only during the inflammatory or proliferative stages but also during the remodelling stage.
Abstract 15912: Cardiac I-123 Metaiodobenzylguanidine Imaging Provides the Additional Prognostic Information to the Model for End-Staged Liver Disease in Patients With Chronic Heart Failure
Authors: Hideyuki Hakui, Takahisa Yamada, Takashi Morita, Yoshio Furukawa, Shunsuke Tamaki, Yusuke Iwasaki, Masato Kawasaki, Atsushi Kikuchi, Takumi Kondo, Tsutomu Kawai, Satoshi Takahashi, Masashi Ishimi, Tatsuhisa Ozaki, Yoshihiro Sato, Masahiro Seo, Masatake Fukunami
Journal: Circulation (2014)

Backgrounds: Liver dysfunction has a prognostic impact on the outcome of patients with advanced heart failure. A model of end-stage liver disease (MELD) is a robust scoring system of liver dysfunction, and a high score has been shown to be associated with poor prognosis in ambulatory patients with heart failure. In addition, cardiac metaiodobenzylguanidine (MIBG) imaging provides prognostic information in patients with chronic heart failure (CHF). However, the long-term predictive value of combining MELD score and cardiac MIBG imaging in CHF patients has not been elucidated. We sought to investigate whether cardiac MIBG imaging provides additional prognostic information to MELD score in CHF patients. Methods: We studied 109 CHF outpatients (NYHA class:2.1±0.6, ischemic origin:52%) with left ventricular ejection fraction27%) had a significantly greater risk of the cardiac events than did those with normal WR for both with high MELD score ([[Unable to Display Character: &amp;#61619;]]10) (70% [19/27] vs 31% [4/13], p=0.028, hazard ratio 4.0 [95%CI 1.2-13.6]) and low MELD score (&lt;10) (34%[10/29] vs 8%[3/40], p=0.005, hazard ratio 6.4 [95%CI 1.7-23.2]). Conclusion: Cardiac MIBG imaging would provide the additional prognostic information to MELD score for CHF patients.
Pneumatic Retinopexy
Authors: Paul E. Tornambe, George F. Hilton, Paul E. Tornambe, Lon S. Poliner, George F. Hilton, Daniel A. Brinton, Timothy P. Flood, David H. Orth, Kirk H. Packo, Stuart Green, Steven R. Leff, Leo Masciulli, David L. Yarian, W. Sanderson Grizzard, Mark E. Hammer, Douglas L. Taren, Craig M. Morgan, James S. Tiedeman
Journal: Ophthalmology (1989)
Abstract P171: Ethnic Inequalities In Cardiac Revascularization After Acute Coronary Syndrome
Authors: Louise Van Oeffelen, Ilonca Vaartjes, Kariien Stronks, Michiel Bots, Charles Agyemang
Journal: Circulation (2014)

Background: Extensive literature in the USA reports lower revascularization rates after coronary heart disease among African Americans than among White Americans. Ethnic differences in revascularization procedures in European countries with an egalitarian health care system are rarely studied. We investigated disparities in revascularization procedures after an acute coronary syndrome (ACS) between ethnic minority groups and the Dutch majority population. Methods: All ACS events between 2006 and 2011 were identified in the Agis insurance Health Database using Diagnosis Treatment Combinations. Multivariable logistic regression analyses (adjusted for age, sex, ACS type, degree of urbanisation, socioeconomic status and comorbidity) were used to identify ethnic differences in revascularization procedures (percutaneous coronary intervention (PCI) and coronary artery bypass grafting (CABG)) within 30 days after the ACS event. Results: On average, after 41.3% of all ACS events a revascularization procedure was performed. In the fully adjusted model, no difference in revascularization rate between the Dutch majority population and the ethnic minority groups under study were found (Moroccan (1.05; 0.77-1.43), Turkish (0.94; 0.75-1.18), Hindustani Surinamese (0.92; 0.70-1.21), and non-Hindustani Surinamese (1.15; 0.61-2.17)). Also age-stratified results did not show significant differences. Conclusion: In the Netherlands, a country with an egalitarian health care system, no ethnic inequalities in revascularization rate within 30 days after an acute coronary syndrome were observed. ACS type, degree of urbanisation, SES, and comorbidity did not influence the findings. Future research should elucidate whether disease severity may have obscured results.
Abstract 49: Low Incidence of Ventricular Tachycardia/Fibrillation as the First Presenting Rhythm in Patients with Out-of-Hospital Cardiac Arrest: Evidence from the Taichung Sudden Unexpected Death Registry (THUNDER) in East Asia
Authors: Yen-Nien Lin, Shih-Sheng Chang, Lee-Min Wang, Kwo-Chang Ueng, Chin-Feng Tsai, Chee-Seong Phan, Li-Hua Lu, Choon-Hoon Hii, Yu-Ting Chung, Jia-Ling Hsieh, Chao-Lin Tsai, Tsu-Juey Wu, Kuan-Cheng Chang
Journal: Circulation (2014)

Background:  Ventricular tachycardia/fibrillation (VT/VF) as the first presenting rhythm is a critical indicator linking to better chance of survival to hospital discharge in patients with out-of-hospital cardiac arrest (OHCA). Compared with a well-documented incidence of 20~30% of VT/VF in Caucasian OHCA patients, the information remains quite limited in East Asian populations. This study aimed to determine the incidence of VT/VF as the first presenting arrhythmia in OHCA patients based on the Taichung Sudden Unexpected Death Registry (THUNDER) in Taiwan.   Methods and Results:  The THUNDER program encompasses a metropolitan city at central Taiwan with a population size of 2.7 million and 17 destination hospitals for OHCA patients. This registry including information on demographics, emergency medical service (EMS) records, and the hospital data according to the Utstein style was constructed using the EMS run sheets of OHCA cases transported by the 119 EMS ambulances. An automated external defibrillator (AED) was routinely used for initial evaluation of each OHCA case. From May 1, 2013 to May 31, 2014, a total of 2156 EMS-assessed OHCAs was analyzed. After excluding 283 cases with non-cardiac OHCA and 114 with incomplete information, the remaining 1759 cases with presumed cardiogenic OHCA constitute the study population. Of these patients, the mean age was 70.4 ± 28.5 years with male predominance (65.2%). The median EMS response time from call help to arrival was 6.0 min (interquartile range: 5–8 min) and the AED machine-read shockable rhythm was registered in 7.7% of all study patients. In a subset of OHCA cases with AED-stored ECG data for analysis (n=1153), the AED-recorded VT/VF, verified by experienced cardiologists, as the first presenting arrhythmia occurred in 8.0% of this subgroup of patients. The overall survival to hospital discharge rate was 3.8%.   Conclusions:  We demonstrate a low incidence of VT/VF in patients with presumed cardiogenic OHCA according to both machine-read and cardiologist-adjudicated VT/VF rhythm. Our findings suggest a different causative mechanism of sudden cardiac death between Asian and Caucasian populations.
Abstract 13322: In-hospital Death and Stroke Following Surgical Aortic Valve Replacement: a Nationwide Perspective
Authors: Shikhar Agarwal, Aatish Garg, Akhil Parashar, E M Tuzcu, Samir R Kapadia
Journal: Circulation (2014)

Significant heterogeneity exists in the current literature around the actual incidence of stroke following aortic valve replacement (AVR) among high-risk patients. We aimed to evaluate the risk of stroke in patients undergoing surgical AVR from a large national database. Methods: We used the 2002-2011 Nationwide Inpatient Sample for this analysis. All patients undergoing isolated AVR or AVR with coronary artery bypass grafting (AVR+CABG) were identified using ICD codes. In-hospital death and any adverse neurological event were co-primary outcomes. Based on predicted death estimates, the study population was classified into low (&lt;8%) and high (≤8%) surgical risk Results: Incidence of in-hospital death was 3.0% and 5.1% among patients undergoing isolated AVR and AVR+CABG respectively. The incidence of adverse neurological event was 2.0% and 2.9% among patients undergoing AVR and AVR+CABG respectively. We demonstrated a progressive increase in the risk of in-hospital death and adverse neurological events with increasing age (Fig 1). The incidence of in-hospital death was as high as 5.7% and 7.4% among patients aged ≤ 85 years undergoing isolated AVR and AVR+CABG respectively. Similarly, the incidence of any adverse neurologic event was 3.2% and 3.6% among patients aged ≤ 85 years undergoing isolated AVR and AVR+CABG respectively. We demonstrated a significantly higher risk of adverse neurological events among the high-risk cohort as compared to the low-risk cohort. Among patients undergoing isolated AVR, the incidence of any adverse neurological event was 1.8% and 4.8% in the low-risk and the high-risk cohorts respectively. Similarly, among patients undergoing AVR+CABG, the incidence of any adverse neurological event was 2.7% and 5.4% in the low-risk and the high-risk cohorts respectively. Conclusions: The incidence of in-hospital death and adverse neurological events was significantly higher among patients with advanced age and elevated surgical risk.
Commentary: Radon blues
Authors: Geoff Watts
Journal: BMJ (2004)

<h3>Abstract</h3> Significant achievements have been made in the fMRI field by pooling statistical results from multiple studies (meta-analysis). More recently, fMRI standardization efforts have focused on enabling the joint analysis of raw fMRI data across studies (mega-analysis), with the hope of achieving more detailed insights. However, it has not been clear if such analyses in the EEG field are possible or equally fruitful. Here we present the results of a large-scale EEG mega-analysis using 18 studies from six sites representing several different experimental paradigms. We demonstrate that when meta-data are consistent across studies, both channel-level and source-level EEG mega-analysis are possible and can provide insights unavailable in single studies. The analysis uses a fully-automated processing pipeline to reduce line noise, interpolate noisy channels, perform robust referencing, remove eye-activity, and further identify outlier signals. We define several robust measures based on channel amplitude and dispersion to assess the comparability of data across studies and observe the effect of various processing steps on these measures. Using ICA-based dipolar sources, we also observe consistent differences in overall frequency baseline amplitudes across brain areas. For example, we observe higher alpha in posterior <i>vs</i> anterior regions and higher beta in temporal regions. We also detect consistent differences in the slope of the aperiodic portion of the EEG spectrum across brain areas. In a companion paper, we apply mega-analysis to assess commonalities in event-related EEG features across studies. The continuous raw and preprocessed data used in this analysis are available through the DataCatalog at https://cancta.net.
Abstract 14954: Circulating Cardiospecific MicroRNAs are Associated with Long-term Prognosis Following Myocardial Infarction
Authors: Olof Gidlof, Gustav J Smith, Kazuma Miyazu, David Erlinge
Journal: Circulation (2011)

Circulating microRNA (miRNA) has recently emerged as a promising biomarker for myocardial infarction. Apoptotic cardiomyocytes release miRNA into the circulation and the detection of cardiospecific...
THE RESULTS OF THE EXPERIMENTAL USE OF SULFUR DIOXIDE IN THE PRODUCTION OF MATERIAL FOR CELL STUDIES IN LUNG CANCER
Authors: W B ALLAN, P WHITTLESEY
Journal: Annals of Internal Medicine (1960)

Article1 February 1960THE RESULTS OF THE EXPERIMENTAL USE OF SULFUR DIOXIDE IN THE PRODUCTION OF MATERIAL FOR CELL STUDIES IN LUNG CANCERWARDE B. ALLAN, M.D., F.A.C.P., PHILIP WHITTLESEY, M.D.WARDE B. ALLAN, M.D., F.A.C.P.Search for more papers by this author, PHILIP WHITTLESEY, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-52-2-326 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptCancer of the lung has become one of the greatest killers we know. The unfortunate feature is that too often this condition is recognized only by chance. When cancer of the lung is diagnosed from history and physical examination, then corroborated by specific tests, it is almost always too late. Even when recognized by chance it is too late. The five-year "cure" in lung cancer after surgery is generally accepted to be in the vicinity of 5%. A recent review of cases at The Johns Hopkins Hospital by one of us revealed the rate to be just under 5%.As... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Baltimore, Maryland*Received for publication May 16, 1959.Presented at the Fortieth Annual Session of The American College of Physicians, Chicago, Illinois, April 21, 1959.From the Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland.With the technical assistance of Miss ELIZABETH B. KELLEY, Baltimore, Maryland.†These studies were made possible by a grant from The American Cancer Society.Requests for reprints should be addressed to Warde B. Allan, M.D., 6 East Eager Street, Baltimore 2, Maryland. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byRole of I-scan technique in screening for lung cancer in smokers with positive sputum cytologyEstablishing the Diagnosis of Lung CancerInitial Diagnosis of Lung CancerPerformance Characteristics of Different Modalities for Diagnosis of Suspected Lung Cancer*Lung Cancer: Perspectives and ProspectsPAUL P. CARBONE, M.D., JOHN K. FROST, M.D., ALVAN R. FEINSTEIN, M.D., GEORGE A. HIGGINS JR., M.D., OLEG S. SELAWRY, M.D.Sputum Induction by Newer Inhalation Methods in Patients with Pulmonary TuberculosisTHE USE OF THE “MEDIHALER” AS AN AID TO BACTERIOLOGICAL DIAGNOSIS IN PULMONARY TUBERCULOSISA review of detection methods for the early diagnosis of lung cancerRecent Advances in the Diagnosis of Bronchogenic CarcinomaClinical Value of Exfoliative Cytology in Neoplasia of the Lung 1 February 1960Volume 52, Issue 2Page: 326-329KeywordsLesionsLung and intrathoracic tumorsPneumoniaSurgery ePublished: 1 December 2008 Issue Published: 1 February 1960 PDF downloadLoading ...
What's in a (Drug) Name? Plenty!
Authors: M Mitka
Journal: JAMA (1999)

THE SIMILARITY IN THE NONPROPRIetary names of two cardiac drugs has resulted in 11 medication errors, including one death, and is compelling authorities to propose name changes. The revisions are being suggested for amrinone and amiodarone. Amrinone is a positive inotropic agent with vasodilator activity, and amiodarone is an antiarrhythmic agent. The United States Pharmacopeia (USP) and the United States Adopted Names Council, which assign generic names to drugs, are proposing changing amrinone to inamrinone—using the “in” prefix that appears intheinnovator’sbrandnameInocor,and changingamiodarone tocamiodarone— usingthe“c”prefixappearingintheinnovator’s brand name Cordarone. The USP said it hoped the name changes incorporating the letter of each innovator’s proprietary name will help health care providers associate the new nomenclature with each drug along with achieving alphabetic separation and creating different verbalization. Amrinone was given its USAN (United States Adopted Name) in 1977 and amiodarone was given its USAN in 1987. Michael Cohen, cofounder and president of the Institute for Safe Medication Practices (ISMP), said his organization has been publishing warnings about the name confusion since the beginning of the decade—before both medications became available as injectables. “This is a big problem because a switch can be fatal,” Cohen said. “They have almost the opposite pharmacological effect, and both are critical care drugs.” Diane D. Cousins, USP vice president for the Practitioner and Product Experience Division, said the USP has monitored the name situation. “A change isn’t going to happen due to a single event, more often it’s a pattern,” Cousins said. “From the early 1990s we couldn’t see a trend, although we knew something was happening and monitored it.”
T cell antigen receptor stimulation induces MALT1 paracaspase–mediated cleavage of the NF-κB inhibitor A20
Authors: Beatrice Coornaert, Mathijs Baens, Karen Heyninck, Tine Bekaert, Mira Haegman, Jens Staal, Lijun Sun, Zhijian J Chen, Peter Marynen, Rudi Beyaert
Journal: Nature Immunology (2008)