tbuckley/cabot-search huggingface dataset:
Immunologic effects of sunitinib in renal cell carcinoma
Authors: C. Lenahan, D. Cho, A. Bissonnette, J. Rosenblatt, Z. Wu, G. Freeman, J. W. Mier, D. Kufe, M. B. Atkins, D. Avigan
Journal: Journal of Clinical Oncology (2008)

14551 Background: Sunitinib is a multi-targeted tyrosine kinase inhibitor with proven efficacy against renal cell carcinoma (RCC). Sunitinib demonstrates immunomodulatory characteristics which may potentially augment the potency of tumor vaccines. We examined the effect of sunitinib therapy on measures of cellular immunity and its ability to reverse tumor associated immune suppression mediated by regulatory T cells (Tregs) and the inhibitory molecule PD-L1 (programmed cell death ligand-1). Methods: Peripheral blood was collected in RCC patients pre- and post-sunitinib therapy. Phenotypic and functional characteristics of circulating T cells and dendritic cells (DC) were determined. Levels of Tregs (CD4+/CD25+/FOXP3+) were quantified. In vitro effects of sunitinib on the expression PD-L1 by RCC were assessed by flow cytometry and western blot analysis. Results: A significant increase in T cell activity was observed after one month of sunitinib therapy as manifested by enhanced proliferative responses to phytohemagglutinin (1.7 fold, p=0.05), allogeneic DCs (2.8 fold) and tetanus toxoid (3.7 fold). However, T cell proliferation was not enhanced by direct exposure to sunitinib in vitro. Sunitinib therapy resulted in a 2.5 fold decrease (p=0.03) in circulating levels of Tregs. In vitro exposure of RCC cell lines to sunitinib resulted in significant depression of PD-L1 expression (11.6 fold decrease in % and 2.5 fold decrease in MFI, p=0.004). In contrast, an increase was seen after exposure to sorafenib. A decrease in PD-L1 expression in RCC tumors treated with sunitinib was also seen in a mouse xenograft model. Coculture of sunitinib with the DC/RCC fusion vaccine resulted in increased capacity to stimulate T cell proliferation (mean stimulation index of 9.5) as compared to fusions alone (mean SI 3.7) and sunitinib alone (mean SI 1.2). Sunitinib exposure also resulted in an increase in fusion mediated stimulation of T cell IFNγ expression. Conclusions: Sunitinib therapy augments measures of T cell immunity by modulating immunosuppressive factors. Levels of Tregs are decreased in vivo and RCC expression of PD-L1 is decreased in vitro. Sunitinib enhances the potency of the DC/RCC fusion vaccine in vitro. These experiments lay the groundwork for a clinical trial of sunitinib with a DC based vaccine in patients with RCC. No significant financial relationships to disclose.
Breast Cancer Screening among Women from 65 to 74 Years of Age in 1987-88 and 1991
Authors: Elizabeth Ann Coleman, Eric J. Feuer
Journal: Annals of Internal Medicine (1992)

Objective: To compare breast cancer screening rates from the 1991 survey with data from 1987-88 for women aged 65 to 74. Design: Surveys of women from five communities. Settings: Five control communities of the National Cancer Institute's Breast Cancer Screening Consortium. Participants: White, non-Hispanic women, ages 65 to 74; 499 in 1987-88 and 2156 in 1991. Response rates for the first survey wave ranged by area from 65% to 77% and for the second survey wave, from 62% to 85%. Main Outcome Measure: Mammogram and clinical breast examination during the past year and performance of monthly breast self examination, with the screening rates in wave 2 directly standardized to the income and education distribution of wave 1 in each area. Results: Mammography use between waves increased significantly (P < 0.05 after adjusting for education, income, and age) in all but one area (from 19% to 33% in wave 1 to 35% to 59% in wave 2). Among women who had a mammogram, the percent who also had a clinical breast examination decreased between waves from 95% to 85% (P = 0.001). Conclusions: Mammography in older women increased dramatically over 3 years, although the use of clinical breast examination may be decreasing.
GREAT REDUCTION IN HEART SIZE ATTENDING THE CLEARING OF CONGESTIVE HEART FAILURE IN A MAN WITH HYPERTENSIVE AND CORONARY HEART DISEASE
Authors: J H CURRENS, P D WHITE
Journal: Annals of Internal Medicine (1949)

Case Reports1 November 1949GREAT REDUCTION IN HEART SIZE ATTENDING THE CLEARING OF CONGESTIVE HEART FAILURE IN A MAN WITH HYPERTENSIVE AND CORONARY HEART DISEASEJAMES H. CURRENS, M.D., PAUL D. WHITE, M.D., F.A.C.P.JAMES H. CURRENS, M.D.Search for more papers by this author, PAUL D. WHITE, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-31-5-912 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe treatment of many patients with cardiac dropsy has been discouraging in the past in spite of the use of digitalis and mercurial diuretics which oftentimes give striking temporary improvement. In the majority of patients with congestive failure resulting from hypertensive and coronary heart disease, however, experience has shown that edema and dyspnea return with monotonous regularity even though activities are kept at a minimum. In the past few years the importance of a low sodium dietary intake has become increasingly apparent as a means of preventing the re-appearance of all the signs of congestive heart failure, particularly among the...Bibliography1. SCHROEDER HA: Studies in congestive heart failure, Am. Heart Jr., 1941, xxii, 141. CrossrefGoogle Scholar2. PROGERGINSBURGMAGENDANTZ SEH: Effects of ingestion of excessive amounts of sodium chloride and water on patients with heart disease, Am. Heart Jr., 1942, xxiii, 555. CrossrefGoogle Scholar3. ELLIS LB: Relative importance of salt and fluid in the management of congestive heart failure, Trans. New Eng. Heart Assoc., 1942, 33-34. Google Scholar4. SCHEMM FR: High fluid intake in management of edema, especially cardiac edema. I. Details and bases of regime, Ann. Int. Med., 1942, xvii, 952; High fluid intake in management of edema, especially cardiac edema. II. Clinical observations and data, Ann. Int. Med., 1944, xxi, 937. LinkGoogle Scholar5. WHEELERBRIDGESWHITE EOWCPD: Diet low in salt (sodium) in congestive heart failure, Jr. Am. Med. Assoc., 1947, xvi, 133. Google Scholar6. KEMPNER W: Treatment of cardiac failure with the rice diet, North Carolina Med. Jr., 1947, viii, 128. Google Scholar7. KARRELL P: De le cure de lait, Arch. gén. de méd., 1866, ii, 513. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Boston, Massachusetts*Received for publication August 28, 1947. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 November 1949Volume 31, Issue 5Page: 912-917KeywordsCoronary heart diseaseDiureticsDyspneaEdemaHeart failurePatientsSodium ePublished: 1 December 2008 Issue Published: 1 November 1949 PDF downloadLoading ...
Abstract 18: Prohibitin 1 regulates apoptosis via its interaction with XIAP
Authors: Yingjie Xu, Wen Yang, Virginia Guarani, Jinjun Shi, J. Wade Harper, Bruce R. Zetter
Journal: Cancer Research (2015)

Abstract Prohibitin 1 (PHB1) belongs to an evolutionarily highly conserved family of proteins that share the stomatin/prohibitin/flotillin/HflK/C (SPFH) domain. PHB1 has been implicated in the regulation of diverse cellular processes, including cell-cycle progression, transcriptional regulation, apoptosis, mitochondrial biogenesis, and as a cell-surface receptor. To better understand the function of PHB1, we analyzed PHB1 protein interactors using Immunoprecipitation followed by mass spectrometry analysis (IP-MS). We identified the X-linked inhibitor of apoptosis protein (XIAP) as a novel PHB1-binding partner. The interaction of PHB1-XIAP has been confirmed both in vitro and in vivo. Using immunofluorescence staining, We observed that PHB1 and XIAP colocalized upon induction of apoptosis. Using various XIAP mutant constructs, we showed that PHB1 binds principally to the BIR3 domain of XIAP. In addition, silencing of either PHB1 or XIAP sensitized cells to chemotherapeutic drug-induced cell apoptosis and partially rescued the chemosensitivity of resistant cancer cells. The observation that PHB1 silencing decreased XIAP levels with a concomitant increase in XIAP cleavage suggests that the formation of a PHB1-XIAP complex inhibits apoptosis by enhancing XIAP stability. Our findings indicate that the PHB1-XIAP complex promotes an anti-apoptotic response in cancer cells and reinforces the potential of PHB1 as a therapeutic target. Citation Format: Yingjie Xu, Wen Yang, Virginia Guarani, Jinjun Shi, J. Wade Harper, Bruce R. Zetter. Prohibitin 1 regulates apoptosis via its interaction with XIAP. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 18. doi:10.1158/1538-7445.AM2015-18
A Golden Rule: Remember the Gift
Authors: E M Hundert
Journal: JAMA (2001)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 11322: Detection of Subclinical Left Ventricular Systolic Dysfunction by Real-Time 3D Speckle Tracking Echocardiography in Patients With Myocardial Bridge of Left Anterior Descending Coronary Artery
Authors: Jing Ping Sun, Dian Wang, Xing Sheng Yang, Alex Pui-Wai Lee, Gengshan Ma, Nai Feng Liu, Cheuk-Man Yu
Journal: Circulation (2014)

Background: To understand the impact of myocardial bridge (MB) on left ventricle (LV) function, we evaluate the myocardial function in patients with MB of the left anterior descending coronary artery (LAD), using real-time 3-dimensional speckle tracking echocardiography (RT3D-STE). Methods: The LAD MB was diagnosed by coronary angiography in 82 patients. According to the percentage of systolic compression (SC), patients were divided into 3 groups: SC &lt;50% defined as Group I (n=24), SC 50%-75% as Group II (n=28), and SC &gt;75% as Group III (n=30). Thirty age and gender matched normal subjects were studied as controls. The traditional echocardiography was performed, and LV deformation was estimated by RT3D-STE. Results: The LV ejection fraction was normal in patients with LAD MB. However, the peak longitudinal (LS) and area strain (AS) of the LAD territory were significantly reduced in Group II and III when compared with controls and Group I (p&lt;0.05). The peak circumferential (CS) and radial strain (RS) were significantly reduced only in Group III (p&lt;0.05) (Table). Furthermore, the amplitude of strain variables were significantly reduced in patients with symptom than those without (AS: 17.7±6.4 vs. 25.3±8.4%; LS:-13.0±1.9 vs. -16.2±2.5%; CS:-15.4±3.3 vs. 18.9±3.0 %; RS: 36.6±3.8 vs. 49.4±2.7%; all p&lt;0.0001). The severity of LAD compression correlated the best with with AS at LAD territory (r=–0.90, p&lt;0.0001), although it was also significant in conventional strain variables (LS: r =–0.79, CS: r =–0.69, RS: r =–0.64, all p &lt;0.0001). Conclusions: Despite apparently normal ejection fraction, myocardial strain is abnormal in the LAD territory which had MB. Such abnormality is more pronounced in patients with progressively more severe MB and in those with symptoms. Furthermore, the area strain by RT3D-STE appeared to be most accurate in detection of subclinical systolic dysfunction in patients with LAD MB than conventional 2D strain parameters.
Taking acute stroke care seriously
Authors: C. Wolfe
Journal: BMJ (2001)

In the United Kingdom government's targets for health improvement stroke is an also ran after cardiovascular disease and is subsumed into the national service framework for older people.1 Gross inequity exists in the provision of stroke services within the UK, and comparisons with mainland Europe show Britain in a poor light. 2 3 These statistics are of concern as the impact of stroke is set to increase. UK incidence data have been used to estimate that there will be a 30% absolute increase in the number of patients experiencing a first ever stroke in 2010 compared with 1983.4

In a recent population based study of survival after stroke in a London population compared with two central European populations survival in London was significantly worse after adjustment for demographic and casemix factors.5 In a separate European hospital based study comparing 12 centres in seven countries similar poor survival rates were observed in UK centres after adjustment for casemix and demographic factors.9 Furthermore, in the International Stroke Trial UK centres had the poorest survival rates in the world.6 …
Cre‐ativity in the liver: Transgenic approaches to targeting hepatic nonparenchymal cells
Authors: Stephen N. Greenhalgh, Kylie P. Conroy, Neil C. Henderson
Journal: Hepatology (2014)

Rapid evolution in transgenic (Tg) mouse technology now permits cell‐specific and temporal control of fluorescent cell‐labeling and gene inactivation. Here, we discuss the principal strategies that have been utilized to target, label, and manipulate hepatic nonparenchymal cells, with emphasis on the utility of constitutive and inducible Cre‐lox systems. We summarize key findings of studies employing Tg technology to target hepatic stellate cells, myofibroblasts, liver sinusoidal endothelial cells, and macrophages to illustrate the power of these approaches in identifying cell‐specific molecular mechanisms critical to the pathophysiology of liver disease. Increasing adoption of Tg techniques will help to answer fundamental questions regarding the pathogenesis of hepatic diseases and provide the mechanistic rationale to allow identification of novel drug targets, ultimately translating into effective therapies for patients with liver disease. (H epatology 2015;61:2091–2099)
Abstract 17: Global diet quality among adults in 187 countries
Authors: Fumiaki Imamura, Shahab Khatibzadeh, Renata Micha, Saman Fahimi, Peilin Shi, John W Powles, Dariush Mozaffarian
Journal: Circulation (2014)

Background: Global quality and trends over time of dietary patterns, a priority for reducing chronic diseases, are unknown. Methods: We evaluated age- and sex-specific energy-adjusted consumption, derived from 325 national surveys covering 88.7% of the global adult population, of 17 key foods and nutrients in 187 countries in 1990 and 2010. Diet patterns were assessed by two distinct scales, one based on higher intakes of healthful items (higher intakes=higher scores), and the other on lower intakes of unhealthful items (lower intakes=higher scores), by country, age, sex, and national income. Results: In 2010, global diet patterns varied substantially ( Figure ). African and South Asian nations had low scores based on more healthful items, but high scores based on fewer unhealthful items. In contrast, Western countries had moderate scores based on healthful items and very poor scores based on unhealthful items. This distinction between more healthful items and fewer unhealthful items was largely masked if we aggregated the two pattern scores, which were weakly correlated ( r =-0.08). By age and sex, women and older adults had higher scores based on either healthful or unhealthful items ( p &lt;0.05). Compared to low-income countries, high-income countries had higher scores based on more healthful items (+4.4 [95% uncertainty interval: 3.8, 5.0]), but substantially lower scores based on fewer unhealthful items (-28.1 [-27.3, -28.9]). From 1990 to 2010, most regions improved their diets based on more healthful items (global average=+2.2 [2.0, 2.4]), except for poorest nations; conversely, nearly all regions worsened their diets based on fewer unhealthful items (-2.5 [-2.8, -2.2]), with marked heterogeneity across global regions. Conclusions: Diet quality varies substantially worldwide, with important differences by higher intakes of healthier foods vs. lower intakes of unhealthy foods. The findings emphasize distinct, nation-specific policy priorities for increasing healthful and decreasing unhealthful foods.
Antiproliferative effect of the combined treatment of celecoxib with docetaxel liposomes in human lung adenocarcinoma cell line
Authors: Madhu S. Shaik, Abhijit Chatterjee, Mandip Singh
Journal: Cancer Research (2005)

5315  The purpose of the study was to investigate the effect of combined treatment of celecoxib with docetaxel liposomes on the cell proliferation, apoptosis and expression of various genes involved in the cell proliferation in A549 lung tumor cell line. Docetaxel liposomes were prepared using dipalmitoylphosphatidylcholine: dipalmitoylphosphatidylethanolamine coupled to polyethylene glycol 2000: phosphatidylcholine: cholesterol: docetaxel by film evaporation method. The drug content in docetaxel liposomes was estimated using HPLC. The lung tumor A549 cells were treated with celecoxib alone (10 μg/ml), docetaxel liposomes (0.01 μg/ml) alone and the combination of celecoxib (10 μg/ml) with docetaxel liposomes (0.01 μg/ml) and the cytotoxicity and apoptosis was assessed by crystal violet dye uptake method and TUNEL staining method, respectively. The expression of cyclooxygenase (COX)-2 and peroxisome proliferator activated receptor (PPAR)-γ was elucidated by RT-PCR. Further, DNA microarray analysis using cancer pathfinder gene array was used to study the expression of various genes involved in cell proliferation and apoptosis. Our results indicate that the delivery of docetaxel using liposomes had a lower IC50 value (0.009 μg/ml) against docetaxel delivered as a solution (0.023 μg/ml). The combined treatment of celecoxib with docetaxel liposomes showed a great antiproliferative effect (IC50 0.004 μg/ml) as compared to all other treatments. TUNEL analysis showed more than 50% apoptosis in cells treated with celecoxib with docetaxel liposomes, whereas celecoxib or docetaxel liposmes showed only 30% apoptosis. There was a significant increase in the expression of PPAR-γ by the combination of celecoxib with docetaxel liposomes, whereas the same combination has minimal effect on COX-2 expression as studied by RT-PCR. DNA microarray analysis indicated that upregulation of various genes such as tumor protein p53, epidermal growth factor receptor and down regulation of cyclin dependent kinase (CDK4) and cyclin dependent kinase inhibitor (p27kip1). In conclusion, our study indicates that the combination treatment of celecoxib with docetaxel liposomes had a greater in-vitro antitumor effect, which may be further explored in the treatment of lung cancer.
Implementing the FDA Modernization Act
Authors: C Marwick
Journal: JAMA (1998)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abnormal DNA methylation in peripheral blood mononuclear cells from patients with vitiligo
Authors: M. Zhao, F. Gao, X. Wu, J. Tang, Q. Lu
Journal: British Journal of Dermatology (2010)

Background Vitiligo is a skin disorder characterized by the destruction of melanocytes by autoreactive lymphocytes. The genetic and environmental factors that trigger the autoimmune response are poorly understood. However, alterations to epigenetic DNA methylation patterns contribute to many other autoimmune diseases. Objectives To investigate genomic and gene‐specific DNA methylation levels in peripheral blood mononuclear cells (PBMCs) of patients with vitiligo and to relate any changes to the expression of genes that regulate methylation, as well as the autoimmune‐related gene IL10. Methods We quantified global methylcytosine levels in PBMCs from 20 patients with vitiligo and 20 healthy controls. mRNA levels of DNA methyltransferases (DNMTs), methyl‐DNA binding domain proteins (MBDs) and interleukin (IL)‐10 were measured by real‐time reverse transcriptase‐polymerase chain reaction. Methylation of an IL10 regulatory element domain was determined by bisulphite genomic sequencing. Results Genomic DNA methylation in PBMCs of patients with vitiligo was increased relative to healthy controls (P =0·012). DNMT1, MBD1, MBD3, MBD4 and MeCP2 expression was significantly higher than in control PBMCs (P =0·013, 0·001, 0·005, 0·001 and 0·001, respectively). MBD1 and MBD3 expression correlated positively with global DNA methylation in vitiligo PBMCs (MBD1: r =0·519, P =0·019; MBD3: r =0·529, P =0·016). IL10 expression was significantly decreased (P =0·030), and an IL‐10 enhancer region was hypermethylated in vitiligo PBMCs compared with controls (P =0·014). Conclusions These data show that levels of DNA methylation are altered in PBMCs of patients with vitiligo, and this may contribute to disease activity by affecting the expression of autoimmunity‐related genes.
Angiographic Versus Functional Severity of Coronary Artery Stenoses in the FAME Study
Authors: Pim A.L. Tonino, William F. Fearon, Bernard De Bruyne, Keith G. Oldroyd, Massoud A. Leesar, Peter N. Ver Lee, Philip A. MacCarthy, Marcel van't Veer, Nico H.J. Pijls
Journal: Journal of the American College of Cardiology (2010)
Abstract 11543: Matrix Metalloproteinase-9 Deletion Attenuates Myocardial Fibrosis and Diastolic Dysfunction in Aging Mice
Authors: Ying Ann Chiao, Yu-Fang Jin, Patricia Shamhart, Rogelio Zamilpa, Qiuxia Dai, Trevi A Ramirez, Jianhua Zhang, Merry L Lindsey
Journal: Circulation (2011)

Ying Ann Chiao, 2011 Finalist and Presenting Author
Rickettsia rickettsii, Rickettsia felis and Rickettsia sp. TwKM03 infecting Rhipicephalus sanguineus and Ctenocephalides felis collected from dogs in a Brazilian spotted fever focus in the State of Rio De Janeiro/Brazil
Authors: F.S. Gehrke, G.S. Gazeta, E.R. Souza, A. Ribeiro, M.T. Martelli, T.T. S. Schumaker
Journal: Clinical Microbiology and Infection (2009)