tbuckley/cabot-search huggingface dataset:
Mesenchymal Stromal Cells for Linked Delivery of Oncolytic and Apoptotic Adenoviruses to Non-small-cell Lung Cancers
Authors: Valentina Hoyos, Francesca Del Bufalo, Shigeki Yagyu, Miki Ando, Gianpietro Dotti, Masataka Suzuki, Lisa Bouchier-Hayes, Ramon Alemany, Malcolm K Brenner
Journal: Molecular Therapy (2015)

Oncolytic adenoviruses (OAdV) represent a promising strategy for cancer therapy. Despite their activity in preclinical models, to date the clinical efficacy remains confined to minor responses after intratumor injection. To overcome these limitations, we developed an alternative approach using the combination of the OAdv ICOVIR15 with a replication incompetent adenoviral vector carrying the suicide gene of inducible Caspase 9 (Ad.iC9), both of which are delivered by mesenchymal stromal cells (MSCs). We hypothesized that coinfection with ICOVIR15 and Ad.iC9 would allow MSCs to replicate both vectors and deliver two distinct types of antitumor therapy to the tumor, amplifying the cytotoxic effects of the two viruses, in a non-small-cell lung cancer (NSCLC) model. We showed that MSCs can replicate and release both vectors, enabling significant transduction of the iC9 gene in tumor cells. In the in vivo model using human NSCLC xenografts, MSCs homed to lung tumors where they released both viruses. The activation of iC9 by the chemical inducer of dimerization (CID) significantly enhanced the antitumor activity of the ICOVIR15, increasing the tumor control and translating into improved overall survival of tumor-bearing mice. These data support the use of this innovative approach for the treatment of NSCLC. Oncolytic adenoviruses (OAdV) represent a promising strategy for cancer therapy. Despite their activity in preclinical models, to date the clinical efficacy remains confined to minor responses after intratumor injection. To overcome these limitations, we developed an alternative approach using the combination of the OAdv ICOVIR15 with a replication incompetent adenoviral vector carrying the suicide gene of inducible Caspase 9 (Ad.iC9), both of which are delivered by mesenchymal stromal cells (MSCs). We hypothesized that coinfection with ICOVIR15 and Ad.iC9 would allow MSCs to replicate both vectors and deliver two distinct types of antitumor therapy to the tumor, amplifying the cytotoxic effects of the two viruses, in a non-small-cell lung cancer (NSCLC) model. We showed that MSCs can replicate and release both vectors, enabling significant transduction of the iC9 gene in tumor cells. In the in vivo model using human NSCLC xenografts, MSCs homed to lung tumors where they released both viruses. The activation of iC9 by the chemical inducer of dimerization (CID) significantly enhanced the antitumor activity of the ICOVIR15, increasing the tumor control and translating into improved overall survival of tumor-bearing mice. These data support the use of this innovative approach for the treatment of NSCLC.
Pulsed intravenous cyclophosphamide and methylprednisolone therapy in refractory pemphigus
Authors: M. Saha, A-M. Powell, B. Bhogal, M.M. Black, R.W. Groves
Journal: British Journal of Dermatology (2009)

Background Pemphigus is a rare autoimmune blistering disorder. The mainstay of current treatment is high-dose oral corticosteroid therapy in combination with a steroid-sparing agent. Adjuvant therapy is important for disease control and to reduce the iatrogenic effects of oral prednisolone. Pulsed therapy with intravenous methylprednisolone and cyclophosphamide (PPC) has been shown to be an effective treatment but there are currently few data on its use in patients who have failed to respond to conventional immunosuppression. Objectives To report the clinical and immunological responses of 21 patients with pemphigus refractory to prednisolone and azathioprine or mycophenolate mofetil treated in our department with a standard protocol of monthly PPC. Methods Patients with pemphigus were identified who had undergone PPC therapy during the period between 1997 and 2006. Initial clinical severity and response to treatment was assessed. In addition, change in intercellular antibody titres and desmoglein 1 and 3 antibodies to PPC therapy was also recorded. Results Of the 21 patients treated, seven had an excellent response, two a good response, five a moderate response, six a minimal response and one patient had no clinical response. Four patients achieved complete clinical remission and the number of pulses for these patients varied between 11 and 22. We observed significant reductions in indirect immunofluorescence titres for normal human skin substrate (P = 0·0078) and antidesmoglein 1 and 3 autoantibody levels (P = 0·007 and P = 0·0085, respectively) from pre-PPC therapy to 1 year after the last pulse. All patients were able to reduce their prednisolone dose from a pre-pulsing median dose of 40–10 mg at the last pulse with a median dose reduction of 66% (P < 0·001). The most common adverse effect was transient lymphopenia (12 patients); nonlife-threatening sepsis (seven patients) and premature ovarian failure (two patients) also occurred. Conclusions PPC can be an effective treatment for refractory pemphigus but its adverse effects should be considered prior to therapy and closely monitored in patients on treatment.
Mouse allergen exposure and immunologic responses*1IgE-mediated mouse sensitization, mouse-specific IgG and IgG4
Authors: Elizabeth C. Matsui, Esmeralda J. M. Krop, Gregory B. Diette, Rob C. Aalberse, Abigail L. Smith, Peyton A. Eggleston
Journal: Journal of Allergy and Clinical Immunology (2004)
THE NEPHROSIS SYNDROME ASSOCIATED WITH TERMINAL UREMIA
Authors: Paul H. Wosika
Journal: Annals of Internal Medicine (1936)

Case Reports1 September 1936THE NEPHROSIS SYNDROME ASSOCIATED WITH TERMINAL UREMIAPAUL H. WOSIKA, M.D.PAUL H. WOSIKA, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-10-3-403 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptNumerous cases of so-called "pure," "genuine" or "lipoid" nephrosis have been described in the literature. A close inspection of these reveals that very few cases in adults conform to the rigid rules clearly stated by Leiter1in his review of the subject in 1931. Using the clinical criteria mentioned as necessary to establish the diagnosis, several authors have published reports of groups of cases of nephrosis, whereas other authors equally interested in the problem of renal disease state that true "lipoid nephrosis" has never been observed by them. Christian2in summarizing the reported cases, was impressed by the short duration...References1. LEITER L: Nephrosis, Medicine, 1931, x, 135. CrossrefGoogle Scholar2. CHRISTIAN HA: Nephrosis: a critique, Jr. Am. Med. Assoc., 1929, xciii, 23. CrossrefGoogle Scholar3. BELL ET: Lipoid nephrosis, Am. Jr. Path., 1929, v, 587. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Chicago, Illinois*Received for publication August 16, 1935.From the Medical Clinic of the Peter Bent Brigham Hospital, Boston, Mass.Department of Medicine, Northwestern University Medical School, Chicago, Ill. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byDegenerative Nierenerkrankungen 1 September 1936Volume 10, Issue 3Page: 403-410KeywordsPathologistsRenal diseases Issue Published: 1 September 1936 PDF downloadLoading ...
Noncompliance May Cause Half of Antihypertensive Drug "Failures"
Authors: J Stephenson
Journal: JAMA (1999)

As many as half of "failures" of treatment to bring elevated blood pressure down to normal levels may be due to unrecognized lapses by patients in taking antihypertensive drugs as prescribed, according to a new study by a team of researchers from the University of Lausanne, Switzerland.
Evaluation of hGM-CSF/hTNFα surface-modified prostate cancer therapeutic vaccine in the huPBL-SCID chimeric mouse model
Authors: Shouhua Lai, Zhiyong Huang, Yunting Guo, Yunqin Cui, Lei Wang, Weifeng Ren, Furong Ying, Hui Gao, Lingxia He, Tieli Zhou, Jiegen Jiang, Jimin Gao
Journal: Journal of Hematology & Oncology (2015)

To validate its efficacy in the context of the human immune system, a novel therapeutic vaccine of hGM-CSF/hTNFα surface-modified PC-3 cells against human prostate cancer was evaluated in the human peripheral blood lymphocytes-severe combined immunodeficiency (huPBL-SCID) chimeric mouse model. The hGM-CSF or/and hTNFα modified vaccines inhibited prostate cancer growth effectively so as to prolong the mouse survival significantly. The splenocytes from the hGM-CSF/hTNFα vaccine-inoculated mice showed the strongest tumor-specific cytotoxicity against PC-3 cells and the highest production of IFNɤ. These features indicated that type 1 protective immune response was induced efficiently against human prostate cancer and further enhanced through synergetic adjuvant effects of hGM-CSF and hTNFα.
Abstract 701: <i>In vivo</i> anti-metastatic effects of uPAR retargeted oncolytic measles virus
Authors: Yuqi Jing, Julia Zaias, Jaime Merchan
Journal: Cancer Research (2014)

Abstract The urokinase receptor (uPAR) is a clinically relevant target for novel biological therapies. Here, we investigated the antimetastatic effects of oncolytic measles virus retargeting uPAR (MV-uPA) in breast cancer metastases models. Species specificities of recombinant viruses retargeted against human (MV-h-uPA) or mouse uPA (MV-m-uPA) were demonstrated in vitro in human and murine breast cancer cell lines. MV-h-uPA and MV-m-uPA induced in vitro cytotoxicity and replicated in human breast cancer cells (MDA-MB231, MDA-MB436 and MCF-7) and murine mammary cancer cells (4T1), respectively. A syngeneic model of mammary cancer metastases was established by intravenous injection of 4T1 cells (5x10e4 per mouse) expressing highly efficient firefly luciferase into immunocompetent BALB/C mice. Seven days after tumor cell inoculation, MV-m-uPA or vehicle was administered into tumor-bearing mice via the tail vein (virus dose: 1×10e6 TCID50 per injection, three injections, every other day). Metastases progression was assessed by bioluminescence analysis. In this highly aggressive syngeneic model, MV-m-uPA treatment was associated with delay in metastases progression and improvement in survival, compared to controls. These effects were associated with detection of viable viral particles from lungs of mice treated with MV-m-uPA, as well as detection of MV-N staining in lung samples in tumor bearing treated mice. Finally, we validated the above effects in a model of human breast cancer metastases. This model was established by injection of 1×10e6 MDA-MB231-luc2 cells via tail vein into female nude mice. Ten days after tumor cell injection, mice were treated with three IV injections (every other day) of PBS or MV-h-uPA (1×10e6 TCID50). Metastases progression (by bioluminescence evaluation) was markedly inhibited in treated mice, compared to controls, resulting in improvement in survival. In conclusion, systemic administration of MV-uPA is effective in the treatment of breast cancer metastases in immune-competent and immune-deficient model, suggesting that measles virus mediated virotherapy may present a novel and promising strategy for treatment against metastatic breast cancer. Citation Format: Yuqi Jing, Julia Zaias, Jaime Merchan. In vivo anti-metastatic effects of uPAR retargeted oncolytic measles virus. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 701. doi:10.1158/1538-7445.AM2014-701
The type of somatic mutation at APC in familial adenomatous polyposis is determined by the site of the germline mutation: a new facet to Knudson's 'two-hit' hypothesis
Authors: Hanan Lamlum, Mohammad Ilyas, Andrew Rowan, Susan Clark, Victoria Johnson, Jennie Bell, Ian Frayling, Jason Efstathiou, Kevin Pack, Stewart Payne, Rebecca Roylance, Patricia Gorman, Denise Sheer, Kay Neale, Robin Phillips, Ian Talbot, Walter Bodmer, Ian Tomlinson
Journal: Nature Medicine (1999)
Mitochondrial Respiration Controls Lysosomal Function during Inflammatory T Cell Responses
Authors: Francesc Baixauli, Rebeca Acín-Pérez, Carolina Villarroya-Beltrí, Carla Mazzeo, Norman Nuñez-Andrade, Enrique Gabandé-Rodriguez, Maria Dolores Ledesma, Alberto Blázquez, Miguel Angel Martin, Juan Manuel Falcón-Pérez, Juan Miguel Redondo, Jose Antonio Enríquez, Maria Mittelbrunn
Journal: Cell Metabolism (2015)
Public health will have priority under UK presidency The NHS has massive staff shortages, say Liberal Democrats Juniors are concerned at new training proposals
Authors: Linda Beecham
Journal: BMJ (1998)

<h3>Abstract</h3> Pathway reconstruction has proven to be an indispensable tool for analyzing the molecular mechanisms of signal transduction underlying cell function. Nested effects models (NEMs) are a class of probabilistic graphical models designed to reconstruct signalling pathways from high-dimensional observations resulting from perturbation experiments, such as RNA interference (RNAi). NEMs assume that the short interfering RNAs (siRNAs) designed to knockdown specific genes are always on-target. However, it has been shown that most siRNAs exhibit strong off-target effects, which further confound the data, resulting in unreliable reconstruction of networks by NEMs. Here, we present an extension of NEMs called probabilistic combinatorial nested effects models (pc-NEMs), which capitalize on the ancillary siRNA off-target effects for network reconstruction from combinatorial gene knockdown data. Our model employs an adaptive simulated annealing search algorithm for simultaneous inference of network structure and error rates inherent to the data. Evaluation of pc-NEMs on simulated data with varying number of phenotypic effects and noise levels demonstrates improved reconstruction compared to classical NEMs. Application to <i>Bartonella henselae</i> infection RNAi screening data yielded an eight node network largely in agreement with previous works, and revealed novel binary interactions of direct impact between established components. <b>Availability</b>: The software used for the analysis is freely available as an R package at https://github.com/cbg-ethz/pcNEM.git <b>Contact</b>: niko.beerenwinkel@bsse.ethz.ch
Practical Anaesthesia and Analgesia for Day Surgery, by J M Millar, G E Rudkin, M Hitchcock
Authors: M. P Shelly
Journal: BMJ (1997)

J M Millar, G E Rudkin, M Hitchcock Bios Scientific Publishers, £19.95, pp 264 ISBN 1 859 96081 2

Day surgery allows more patients to be treated, improves the efficiency of bed use, and reduces costs. All this is possible because medical practice has changed—for example, early mobilisation after surgery—and because medical technology has advanced to allow techniques such as laparoscopic surgery.

This book gives an up to date account of its subject. It explains the advantages of day surgery to convert those hesitant to accept it and concentrates on how to improve the service offered by those who are already converts. The authors convey their message clearly, with …
Antibiotics in Laboratory Medicine.
Authors: Victor Lorian
Journal: Annals of Internal Medicine (1980)

The Breakpoint 1 Matthew A. Wikler, Paul G. Ambrose Antimicrobial Susceptibility on Solid Media 8 John D. Turnidge, Jan M. Bell Susceptibility Testing of Antimicrobials in Liquid Media 61 Daniel Amsterdam Antimicrobial Susceptibility Testing of Anaerobic Bacteria 144 David W. Hecht Antimycobacterial Agents: In Vitro Susceptibility Testing and Mechanisms of Action and Resistance 155 Clark B. Inderlied and Kevin A. Nash Antifungal Drugs: Mechanisms of Action, Drug Resistance, Susceptibility Testing, and Assays of Activity in Biological Fluids 226 Michael A. Pfaller, Daniel J. Diekema, and Michael G. Rinaldi Antimicrobial Susceptibility Testing for Some Atypical Microorganisms (Chlamydia, Mycoplasma, Rickettsia, Ehrlichia, Coxiella, and Spirochetes) 266 Jean-Marc Rolain, Didier Raoult Applications, Significance of, and Methods for the Measurement of Antimicrobial Concentrations in Human Body Fluids 290 Roger D. Klein and Stephen C. Edberg Antimicrobial Combinations 365 Satish K. Pillai, Robert C. Moellering, Jr., and George M. Eliopoulos Genetic and Biochemical Mechanisms of Bacterial Resistance to Antimicrobial Agents 441 Louis B. Rice and Robert A. Bonomo Molecular Methods for the Detection of Antibacterial Resistance Genes 509 Margareta Ieven Molecular Mechanisms of Action for Antimicrobial Agents: General Principles and Mechanisms for Selected Classes of Antibiotics 532 Charles W. Stratton The Antivirogram and the Modes of Action of Antiviral Agents, HIV, Hepatitis, Influenza, and Cytomegalovirus 564 Pierre Dellamonica, Vincent Calvez, Anne-Genevieve Marcelin, Albert Tran, Pierre-Marie Roger, Marie-Christine Mazeron, and Bruno Lina Disinfectants and Antiseptics, Modes of Action, Mechanisms of Resistance, and Testing 615 Joseph M. Ascenzi, Martin S. Favero Evaluation of Antimicrobials in Experimental Animal Infections 654 Terry O' Reilly, D. A. Andes, Ch. Ostergaard, N. Frimodt-Moller Extravascular Antimicrobial Distribution and the Respective Blood and Urine Concentrations in Humans 719 David M. Bamberger, John W. Foxworth, Darcie L. Bridwell, Christopher S. Shain, and Dale N. Gerding Epidemiology of Antimicrobial Resistance: Species Prevalence, Susceptibility Profiles, and Resistance Trends 815 Thomas R. Fritsche, Helio S. Sader, Ronald N. Jones Index 851
How does it work?: Alcohol breath testing
Authors: A. Berger
Journal: BMJ (2002)

• Drivers are initially tested for alcohol impairment at the roadside with a screening device. If this produces a positive test, evidential breath testing is performed at the police station. Motorists can be stopped and required to take a breath test by police at the scene of a road traffic accident, if a police officer suspects a motorist may be driving under the influence of alcohol, or if a motorist commits a moving traffic offence.



Five breath screening devices are currently approved for police use in Britain. They work on the principle that in the presence of a catalyst, oxygen in a sample of expired air converts any alcohol present into acetic acid and then to water and carbon dioxide. A fuel cell converts the chemical energy released when oxidation occurs into a detectable …
A new glass microfibre‐based histamine analysis for allergy testing in children
Authors: H. Nolte, O. Schiøtz, P. Stahl Skov
Journal: Allergy (1987)

A new microfibre method for allergy testing measuring histamine release from human basophil leukocytes is described. Samples of 50 μl washed blood are challenged with the suspected allergens. Released histamine is bound to microfibres and measured by a spectrofluorometrical method after removal of interfering substances by washing. The microfibre method (HR‐MM) was compared to the conventional histamine release assay using the Ficoll‐Hypaque gradient method (HR‐FH) in 19 allergic children tested with one of three allergens. In addition, a comparison was made between the microfibre method and in vivo provocation tests, i.e. skin prick test (SPT), bronchial provocation test (BPT) and allergen specific serum IgE (RAST). It was found that the same individuals responded with histamine release to the same allergens in both histamine release assays, and the dose‐response curves were almost identical. A positive correlation was found between the in vivo and in vitro tests. Thus it is concluded that the new method can provide reproducible, analytically precise (at the nanogram level) histamine release results in pediatric cases where: 1) a positive SPT does not correlate with case history; 2) BPT may be considered too hazardous or inconvenient; 3) confirmation of negative or inconclusive SPT or RAST is needed. In contrast to other histamine release assays it is a convenient diagnostic tool in children since only small amounts of blood are needed and at least 96 tests can be carried out in 21/2 h
Circulating granulocyte colony-stimulating factor (G-CSF) levels after allogeneic and autologous bone marrow transplantation: endogenous G-CSF production correlates with myeloid engraftment [see comments]
Authors: MS Cairo, Y Suen, L Sender, ER Gillan, W Ho, JM Plunkett, C van de Ven
Journal: Blood (1992)
Improvisation 30 (Cannons)
Authors: M. Therese Southgate
Journal: JAMA (1999)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
HEMOLYTIC CRISIS IN HEREDITARY SPHEROCYTOSIS: STUDY OF A FAMILY OF FIVE WITH CONCURRENT CRISES
Authors: JONAH G. LI, M.D., IRVIN G. VOTH, M.D., EDWIN E. OSGOOD, M.D., F.A.C.P.
Journal: Annals of Internal Medicine (1950)

ExcerptThe pathogenesis of the hemolytic crisis, which occurs so precipitously in hereditary spherocytosis (congenital hemolytic jaundice), is unknown. However, it has been generally accepted that such crises are due to a sudden acceleration of hemolysis.1Recently, Owren2questioned this widely accepted view and proposed a new concept of the pathogenesis of the hemolytic crisis. He contended that the crisis is the result of aplasia of the bone marrow rather than an increased rate of hemolysis. His opinion is based on careful studies by serial blood and sternal marrow examinations of six patients observed through such crises. He noted leukopenia, thrombocytopenia...
Survival After Application of Automatic External Defibrillators Before Arrival of the Emergency Medical System
Authors: Myron L. Weisfeldt, Colleen M. Sitlani, Joseph P. Ornato, Thomas Rea, Tom P. Aufderheide, Daniel Davis, Jonathan Dreyer, Erik P. Hess, Jonathan Jui, Justin Maloney, George Sopko, Judy Powell, Graham Nichol, Laurie J. Morrison
Journal: Journal of the American College of Cardiology (2010)
Distinct regulators control the expression of the mid-hindbrain organizer signal FGF8
Authors: Weilan Ye, Maxime Bouchard, Donna Stone, Xiaodong Liu, Francis Vella, James Lee, Harukazu Nakamura, Siew-Lan Ang, Meinrad Busslinger, Arnon Rosenthal
Journal: Nature Neuroscience (2001)