tbuckley/cabot-search huggingface dataset:
Abstract P420: Dietary Fat on Whole Blood Gene Expression and Plasma Lipids in the Framingham Heart Study
Authors: Michael M Mendelson, Brian Chen, Chunyu Liu, Roby Joehanes, Peter Munson, Gail Rogers, Jose M Ordovas, Alice H Lichtenstein, Serkalem Demissie, L. A Cupples, Sander Robins, Martin G Larson, Paul F Jacques, Dan Levy
Journal: Circulation (2014)

Objective: To describe the influence of type of dietary fat on the activity of metabolic pathways, as measured by gene expression profiles, and the relation to plasma lipids. Background: Metabolic studies have demonstrated strong associations of dietary fatty acid composition with plasma lipids. Relatively little is known about the pathways and gene expression changes that mediate these relationships in the general population. Methods: We analyzed self-reported dietary intake of fatty acids, plasma lipid levels, and genome-wide gene expression data from Framingham Heart Study Offspring and Third Generation cohort participants. We excluded participants on lipid therapy. Multivariable linear regression models were conducted with plasma lipids as separate outcomes, energy-adjusted residuals of dietary fats as predictors, and adjustment for clinical and dietary covariates. Normalized gene expression from whole blood derived RNA was similarly modeled with additional adjustment for cell count and batch effects. Results: Among 3681 participants, higher polyunsaturated fatty acid (PUFA) intake is associated with lower LDL-C (estimated β [regression coefficient] = -0.5, p=0.002), higher HDL-C (β= 0.4, p&lt;0.0001), and lower triglyceride (β= -0.009, p=0.0003) concentrations after adjustment for age, sex, carbohydrate, protein, and alcohol intake. Higher PUFA intake was associated with differential gene expression of cholesterol efflux transporters (ABCA1/ABCG1), LDL receptor degrader (IDOL), and non-lipoprotein metabolism related transcripts (FDR &lt; 0.05). In contrast, higher saturated fat intake (SFA) showed inverse associations with ABCA1 expression levels and HDL cholesterol ( Figure 1 ). Conclusions: Higher PUFA intake is associated with a less atherogenic lipid profile and higher ABCA1 expression with inverse associations for higher SFA intake. Gene expression analysis reveals important links between dietary fat type, specific cholesterol metabolism pathways, and lipids in a community cohort.
Abstract 4357: Long noncoding RNA HOTAIR is targeted and regulated by microRNA-141 in renal carcinoma cells
Authors: Takeshi Chiyomaru, Shinichiro Fukuhara1, Sharanjot Saini, Shahana Majid, Guoren Deng, Varahram Shahryary, Inik Chang, Yuichiro Tanaka, Hideki Enokida, Masayuki Nakagawa, Rajvir Dahiya, Soichiro Yamamura
Journal: Cancer Research (2014)

Abstract HOTAIR is a long non-coding RNA (lncRNA) which interacts with the Polycomb Repressive Complex and suppresses its target genes. HOTAIR has also been demonstrated to promote malignancy. MicroRNA-141 (miR-141) has been reported to play a role in the epithelial to mesenchymal transition (EMT) process and the expression of miR-141 is inversely correlated with tumorigenicity and invasiveness in several human cancers. We found that HOTAIR expression is inversely correlated to miR-141 expression in renal carcinoma cells. HOTAIR promotes malignancy including proliferation and invasion whereas miR-141 suppresses malignancy in renal carcinoma cells. miR-141 binds to HOTAIR in a sequence specific manner, and suppresses HOTAIR expression and functions including proliferation and invasion. Both HOTAIR and miR-141 were associated with the immunoprecipitated Argaunaute2 (Ago2) complex and the Ago2 complex cleaved HOTAIR in the presence of miR-141. These results demonstrate that HOTAIR is suppressed by miR-141 in an Ago2 dependent manner. Citation Format: Takeshi Chiyomaru, Shinichiro Fukuhara1, Sharanjot Saini, Shahana Majid, Guoren Deng, Varahram Shahryary, Inik Chang, Yuichiro Tanaka, Hideki Enokida, Masayuki Nakagawa, Rajvir Dahiya, Soichiro Yamamura. Long noncoding RNA HOTAIR is targeted and regulated by microRNA-141 in renal carcinoma cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4357. doi:10.1158/1538-7445.AM2014-4357
Obesity—can we turn the tide?
Authors: Mike Lean, Laurence Gruer, George Alberti, Naveed Sattar
Journal: BMJ (2006)

<h3>OBJECTIVE</h3> Autoantibodies such as rheumatoid factor (RF), antikeratin antibodies (AKA), antiperinuclear factor (APF), and anti-RA 33 antibodies are considered of value for the diagnosis of RA. The purpose of this study was to evaluate these autoantibodies as predictors of severe radiographic damage in rheumatoid arthritis (RA). <h3>PATIENTS AND METHODS</h3> Eighty six patients with RA (70 women, 16 men) fulfilling 1987 ACR criteria were selected from a cohort of 469 patients followed up since the first year of RA onset because they could be divided in two groups according to the severity of the radiographic damage. These 86 patients had a mean (SD) disease duration of eight (four) years: 43 patients had severe radiographic damage (Larsen score ⩾2) and 43 had limited radiographic damage (Larsen score &lt; 2). The two groups were matched by disease duration and sex. The following autoantibodies were looked for: RF, ANA, AKA, APF, and anti-RA 33 antibodies. In addition, HLA class II DR β alleles and standard inflammatory parameters (erythrocyte sedimentation rate, C reactive protein) were determined. <h3>RESULTS</h3> Patients with severe radiographic damage differed from those with limited radiographic damage in that they had higher RF (p=0.01), APF (p&lt;0.02), and AKA (p=0.001) titres. Stepwise regression analysis was done to calculate the odds ratios (OR) for each clinical and laboratory variable; only presence of cutaneous nodules (OR: 14.9; 95% CI: 7, 128), HLA DRB1*04 or DRB1*01 (OR: 7.53; 95% CI: 1.32, 42.9), AKA (OR: 3.11; 95%, CI: 0.58, 16.8 ), a high erthrocyte sedimentation rate (OR: 2.66; 95% CI: 0.60, 11.9), and a high C reactive protein value (OR: 7.4; 95% CI : 1.43, 38.1) were predictive of severe radiographic damage. <h3>CONCLUSION</h3> These data suggest that the risk of severe radiographic damage in RA patients is higher when cutaneous nodules, HLA DRB1*04 or DRB1*01, and/or AKA are present. The other autoantibodies of diagnostic significance are of little help for predicting joint destruction.
Scientists isolate HIV blocker in placenta
Authors: Scott Gottlieb
Journal: BMJ (2001)

Scientists have isolated a powerful blocker of HIV that is produced in the placenta and may one day be used to prevent mother to child transmission of the virus.

Scientists have known that the molecule, called leukaemia inhibitory factor, has a key role in maintaining pregnancy and in blastocyst implantation, but its potential for inhibiting HIV has not been discovered before now, said Dr Bruce Patterson from the Children's Memorial Hospital in Chicago, Illinois, and associates ( Journal of Clinical Investigation 2001;107:287-94).

The researchers looked at levels of leukaemia inhibitory factor in the placentas of HIV positive women who had transmitted …
Abstract 17883: Gender Differences in the Association of Poor Sleep Quality with Arterial Stiffness: the META-Health Study
Authors: Alanna Morris, Riyaz Patel, Dorothy Coverson, Lucy Fike, Yusuf Ahmed, Neli Stoyanova, W. Craig Hooper, Gary Gibbons, Donald Bliwise, Viola Vaccarino, Rebecca Din-Dzietham, Arshed Quyyumi
Journal: Circulation (2010)

Background: Poor nocturnal sleep quality has been associated with cardiovascular (CVD) morbidity and mortality. This risk may be mediated by nocturnal fluctuations in blood pressure with subsequent...
Murine and human Langerhans cells express a functional histamine H<sub>4</sub> receptor: modulation of cell migration and function
Authors: M. Gschwandtner, K. Rossbach, D. Dijkstra, W. Bäumer, M. Kietzmann, H. Stark, T. Werfel, R. Gutzmer
Journal: Allergy (2009)

Histamine is an important mediator of allergic reactions, and recent studies indicated that the function of different types of antigen presenting cells (APC) can be modulated by histamine, in particular via the newly described histamine H(4) receptor (H(4)R). Therefore, we investigated possible interactions of histamine via the H(4)R on Langerhans cells (LC), which represent the professional APC in the skin and therefore have an important role in the initiation and maintenance of allergic skin diseases.The expression of the H(4)R was evaluated by real-time PCR, flow cytometry and immunofluorescence staining. The function of the H(4)R was determined by intracellular flow cytometric measurement of chemokine production and LC migration assays.Here, we show H(4)R expression on in vitro generated monocyte-derived LC (mRNA and protein) and on primary LC from murine and human skin samples (protein). The immunofluorescence staining in murine and human skin samples clearly proved that LC express the H(4)R in situ. Stimulation with histamine or a H(4)R agonist downregulated the chemokine (C-C motif) ligand 2 (CCL2) in human monocyte-derived LC and primary LC. Prestimulation with a selective H(4)R antagonist abolished this effect. Moreover, migration of LC from the epidermis was increased after H(4)R agonist stimulation in ex vivo migration assays using human epidermis and murine in vivo assays.Our findings show that LC express a functional H(4)R and point towards a possible pathogenic relevance of the H(4)R in inflammatory and allergic diseases.
Benign familial Degos disease worsening during immunosuppression
Authors: None Powell, None Bordea, None Wojnarowska, None Farrell, None Morris
Journal: British Journal of Dermatology (1999)

We describe a 61-year-old woman with skin lesions consistent with those found in Degos disease, both in clinical and in histological appearance. She had had several of these lesions for many years, as had her mother, sister and niece. In 1991, she underwent cadaveric renal transplantation and was treated with immunosuppression: prednisolone, azathioprine and cyclosporin. At that time, she developed many more characteristic skin lesions, and these were slightly larger and more noticeable than those she had had previously. She and the other affected family members appear to fit into the more benign subgroup of Degos disease, and it seems that her immunosuppression aggravated her cutaneous disease.
Symptoms and Signs in Clinical Medicine. An Introduction to Medical Diagnosis.
Authors: E. Noble Chamberlain
Journal: Annals of Internal Medicine (1962)

Although this book is largely a digest of the literature, with little that is new, the common symptoms and physical signs of disease are completely covered. Simple laboratory and instrumental procedures have been gathered together in separate chapters for easy reference. The text is well written and there are numerous splendid illustrations. The chapters on diseases of the heart and vessels and the digestive system are complete and deserve special commendation.
Abstract 2314: Don’t Be Fooled by CRT Device Counters in Patients with AF: Common Presence of Ineffective Biventricular Capture as Determined by Sophisticated Holter Monitoring
Authors: Delia Cotiga, Aysha Arshad, Emad Aziz, Walter Pierce, Jonathan S Steinberg
Journal: Circulation (2006)

BACKGROUND: Atrial fibrillation (AF) is present in a substantial minority of patients with advanced heart failure(HF). Despite special efforts in the setting of CRT devices, loss of sinus rhythm ma...
Activated Factor VII–Antithrombin Complex Plasma Concentration Is An Independent Predictor Of Total and Cardiovascular Mortality In Patients With Coronary Artery Disease and Its Prognostic Significance Is Improved By Using Factor VII Genotype-Specific Threshold Levels
Authors: Nicola Martinelli, Domenico Girelli, Marcello Baroni, Patrizia Guarini, Federica Tosi, Barbara Lunghi, Barry J Woodhams, Francesco Bernardi, Oliviero Olivieri
Journal: Blood (2013)
A memorable patient: Leaving mercy to heaven
Authors: H. Fox
Journal: BMJ (2003)

I was her general practitioner for several years, first as a trainee and then a partner in the practice. Over that time she gradually became more disabled with osteoarthritis and progressed from being a surgery attender to one whom I visited at home when necessary. She used to send me flowers at Christmas. She was one who used to say “Don't get old.” Many say that, but with her I once joked, “It's better than the alternative,” and we shared a laugh.

Later she developed atrial fibrillation and took warfarin. She was intolerant of non-steroidal anti-inflammatory drugs, and we struggled to …
Embryonic stem cells as sources of donor-independent platelets
Authors: Matthew C. Canver, Daniel E. Bauer, Stuart H. Orkin
Journal: Journal of Clinical Investigation (2015)

The creation of a donor-independent source of platelets has been challenging; however, recent advances show growing promise for alternative platelet sources. Pluripotent stem cells have the capacity to differentiate into mature megakaryocytes with the ability to produce functional platelets. In this issue of JCI, Noh et al. provide a proof-of-principle demonstration that embryonic stem cells can be used to produce platelets on a clinical scale by controlling the level of the transcription factor GATA1. This study emphasizes the importance of precise regulation of gene expression for regenerative medicine applications.
Cryosurgery for Hepatic Malignancies
Authors: Daniel J. Gagné, Mark S. Roh
Journal: Liver Cancer (1998)

It was estimated that there would be 131,200 new cases of colon and rectal cancer in 1997 in the United States and 54,900 deaths due to colon and rectal cancer.1 Ninety percent of patients who die from colorectal cancer have liver metastases.2 The liver is the most prevalent site of first or only recurrence.3 Liver metastases occur in 40–75% of patients with colorectal cancer, but only 10–30% have metastatic disease limited to the liver.4,5 Surgical resection of hepatic metastases remains the treatment of choice when feasible, but only 5–10% of patients are considered candidates for liver resection.6,7 Patients with limited liver metastases from colorectal cancer who do not undergo hepatic resection rarely survive more than 2 years after diagnosis.8,9 Five-year survival rates of 25–35% have been reported in patients who have undergone hepatic resection of colorectal metastases.10,11 Unfortunately, at least 75% of patients with limited liver-only metastases are not candidates for surgical resection.12 Patients who have multiple metastases involving both the right and left lobes of the liver, metastatic lesions in proximity to major blood vessels or biliary structures where a 1-cm margin of clearance cannot be achieved, or more than three or four liver metastases have usually been considered to have unresectable lesions and do not qualify for standard hepatic surgical resection.8,10,13,14
Risk of Incident Colorectal Cancer and Death After Colonoscopy: A Population-based Study in Utah
Authors: N. Jewel Samadder, Karen Curtin, Lisa Pappas, Ken Boucher, Geraldine P. Mineau, Ken Smith, Alison Fraser, Yuan Wan, Dawn Provenzale, Anita Y. Kinney, Cornelia Ulrich, Randall W. Burt
Journal: Clinical Gastroenterology and Hepatology (2015)
Evidence grows for safety of mobile phones
Authors: Scott Gottlieb
Journal: BMJ (2001)

Regular use of mobile telephones does not seem to cause an increased incidence of brain tumours, but further studies are needed to account for longer induction periods, especially for slow growing tumours, according to the results of two new studies.

The studies, which together involved more than 1250 patients with brain tumours and an equal number of healthy individuals, found no increased risk of cancers among those who used the devices more frequently. The results are not likely to put the issue of potential harm completely to rest, but they join a growing body of evidence suggesting that the only important risk associated with the handheld devices is a higher likelihood of traffic accidents.

“In all of the available scientific literature, there …
The Extent of Microvascular Damage During Myocardial Contrast Echocardiography Is Superior to Other Known Indexes of Post-Infarct Reperfusion in Predicting Left Ventricular Remodeling
Authors: Leonarda Galiuto, Barbara Garramone, Antonio Scarà, Antonio G. Rebuzzi, Filippo Crea, Giuseppe La Torre, Stefania Funaro, Mariapina Madonna, Francesco Fedele, Luciano Agati
Journal: Journal of the American College of Cardiology (2008)
96. Transgene Expression from Recombinant AAV-8 Vectors in the Liver Occurs Independently of the Serotype Origin of the Viral Inverted Terminal Repeats
Authors: Dirk Grimm, Hiroyuki Nakai, Theresa A. Storm, Efren P. Riu, Mark A. Kay
Journal: Molecular Therapy (2004)

We present data from the long-term in vivo evaluation of gene transfer vectors combining properties from seven different serotypes of Adeno-Associated Virus (AAV), and discuss how they corroborate our current model for liver transduction by AAV.
Acetylcholine receptor M3 domain: stereochemical and volume contributions to channel gating
Authors: Hai-Long Wang, Margherita Milone, Kinji Ohno, Xing-Ming Shen, Akira Tsujino, Anna Paola Batocchi, Pietro Tonali, Joan Brengman, Andrew G. Engel, Steven M. Sine
Journal: Nature Neuroscience (1999)
Cytomegalovirus Reactivation in Critically Ill Immunocompetent Patients
Authors: Ajit P. Limaye, Katharine A. Kirby, Gordon D. Rubenfeld, Wendy M. Leisenring, Eileen M. Bulger, Margaret J. Neff, Nicole S. Gibran, Meei Li Huang, Tracy K. Santo Hayes, Lawrence Corey, Michael Boeckh
Journal: JAMA (2008)

Cytomegalovirus (CMV) infection is associated with adverse clinical outcomes in immunosuppressed persons, but the incidence and association of CMV reactivation with adverse outcomes in critically ill persons lacking evidence of immunosuppression have not been well defined.To determine the association of CMV reactivation with intensive care unit (ICU) and hospital length of stay in critically ill immunocompetent persons.We prospectively assessed CMV plasma DNAemia by thrice-weekly real-time polymerase chain reaction (PCR) and clinical outcomes in a cohort of 120 CMV-seropositive, immunocompetent adults admitted to 1 of 6 ICUs at 2 separate hospitals at a large US tertiary care academic medical center between 2004 and 2006. Clinical measurements were assessed by personnel blinded to CMV PCR results. Risk factors for CMV reactivation and association with hospital and ICU length of stay were assessed by multivariable logistic regression and proportional odds models.Association of CMV reactivation with prolonged hospital length of stay or death.The primary composite end point of continued hospitalization (n = 35) or death (n = 10) by 30 days occurred in 45 (35%) of the 120 patients. Cytomegalovirus viremia at any level occurred in 33% (39/120; 95% confidence interval [CI], 24%-41%) at a median of 12 days (range, 3-57 days) and CMV viremia greater than 1000 copies/mL occurred in 20% (24/120; 95% CI, 13%-28%) at a median of 26 days (range, 9-56 days). By logistic regression, CMV infection at any level (adjusted odds ratio [OR], 4.3; 95% CI, 1.6-11.9; P = .005) and at greater than 1000 copies/mL (adjusted OR, 13.9; 95% CI, 3.2-60; P < .001) and the average CMV area under the curve (AUC) in log(10) copies per milliliter (adjusted OR, 2.1; 95% CI, 1.3-3.2; P < .001) were independently associated with hospitalization or death by 30 days. In multivariable partial proportional odds models, both CMV 7-day moving average (OR, 5.1; 95% CI, 2.9-9.1; P < .001) and CMV AUC (OR, 3.2; 95% CI, 2.1-4.7; P < .001) were independently associated with a hospital length of stay of at least 14 days.These preliminary findings suggest that reactivation of CMV occurs frequently in critically ill immunocompetent patients and is associated with prolonged hospitalization or death. A controlled trial of CMV prophylaxis in this setting is warranted.
Defects in the cappuccino (cno) gene on mouse chromosome 5 and human 4p cause Hermansky-Pudlak syndrome by an AP-3–independent mechanism
Authors: Babette Gwynn, Steven L. Ciciotte, Susan J. Hunter, Linda L. Washburn, Richard S. Smith, Sabra G. Andersen, Richard T. Swank, Esteban C. Dell'Angelica, Juan S. Bonifacino, Eva M. Eicher, Luanne L. Peters
Journal: Blood (2000)
Advancement of scientific knowledge through international collaborative research.
Authors: Miguel R Jorge
Journal: World Psychiatry (2006)

The WPA has experienced a significant growth during the last two decades, particularly regarding its political role and administrative organization, number of Member Societies and Sections, and activities in the fields of meetings, education, and publications.

Particularly, its 65 current Sections - through their leadership and members - are developing different activities such as organizing scientific meetings and sessions within national and international congresses; producing educational material, guidelines and consensus/ position statements; publishing journals and/or books; promoting research, and establishing collaboration with national and international organizations.

The WPA Executive Committee has recently awarded six different Sections' research projects with partial financial support, in order to enhance its institutional involvement in the development of scientific research. During 2005, G. Christodoulou, past WPA Secretary for Sections, and the former Operational Committee for Sections were responsi ble to conduct the selection process and submit some of the research projects to the Executive Committee for a final decision. These projects are summarized below.
Inflammation, Complement Factor H, and Age-Related Macular Degeneration
Authors: Ronald Klein, Michael D. Knudtson, Barbara E.K. Klein, Tien Y. Wong, Mary Frances Cotch, Kiang Liu, Ching Y. Cheng, Gregory L. Burke, Mohammed F. Saad, David R. Jacobs, A. Richey Sharrett
Journal: Ophthalmology (2008)
773. Non-Invasive In Vivo Detection of Episomes Delivered to Mouse Hepatocytes by a Helper Dependent Adenovirus- Epstein-Barr Virus Hybrid Vector System
Authors: Sean D. Gallaher, Jose S. Gil, Oliver Dorigo, Arnold J. Berk
Journal: Molecular Therapy (2004)

Helper Dependent Adenovirus (HDA) vectors deleted in all viral genes persist poorly in vivo due to viral genome loss during mitosis. We have addressed this limitation by using elements from Epstein-Barr Virus (EBV), the genome of which persists as an extra-chromosomal episome in replicating B cells. Maintenance is mediated by the virally expressed Epstein-Barr Nuclear Antigen 1 (EBNA-1) protein that binds the Family of Repeats (FR) region of the EBV episome, and tethers it to metaphase chromosomes for segregation during telophase. In our hybrid binary system, an HDA vector (HDA.EBV) is used to deliver a linear EBV-based episome to target cells. Co-infection with a second HDA expressing Cre recombinase (HDA.Cre) leads to the excision and circularization of the loxP-flanked episome sequence, and places a CMV promoter upstream of the transgene. The episome also contains a human origin and the FR for replication and segregation in mitotic cells. We have shown that co-infection with HDA.Cre and an HDA.EBV vector carrying either a Cyan Fluorescence Protein (CFP) reporter gene or Puromycin AcetylTransferase (PAC) drug resistance gene produces circular episomes in vitro and that EBNA-1 and the FR significantly prolong transgene expression (in publication).