tbuckley/cabot-search huggingface dataset:
Cytoreduction and prognosis in acute lymphoblastic leukemia--the importance of early marrow response: report from the Childrens Cancer Group.
Authors: P G Steinherz, P S Gaynon, J C Breneman, J M Cherlow, N J Grossman, J H Kersey, H S Johnstone, H N Sather, M E Trigg, R Chappell, D Hammond, W A Bleyer
Journal: Journal of Clinical Oncology (1996)

PURPOSE To quantify the residual marrow lymphoblast fraction that best defines patients at high risk for relapse, and the optimal time for assessment during remission induction. PATIENTS AND METHODS The residual lymphoblast percentage was evaluated on day 7 (n = 220) and day 14 (n = 205) during a four- or five-drug induction in patients with poor prognostic factors. The rate of cytoreduction was related to event-free survival (EFS) and other factors. RESULTS On the New York (NY) regimen, 68%, 14%, and 18%, and on the Berlin-Frankfurt-Munster (BFM) regimen, 56%, 15%, and 29% of patients had M1 (&lt; 5% blasts), M2 (5-25%), or M3 (&gt; 25%) responses on day 7 (P = .075). On day 14, the corresponding values were 87%, 6%, 7% on NY and 84%, 8%, 8% on BFM. For patients who achieved remission by day 28 and a day-7 marrow rating of M1, M2, or M3, the 6-year EFS rate was 78%, 61%, and 49% (P &lt; .001). The day-14 ratings predicted for a 72%, 32%, or 40% EFS (P &lt; .001). Patients with 5% to 10% blasts day 7 had three times as many events as those with less than 5% and had no better EFS than those with 11% to 25% blasts. Patients with a WBC count more than 200,000/microL at diagnosis and an M1 day 7 marrow had an EFS rate of 69%, while for those with M2 or M3, the EFS rate was 41%. Day-7 marrow had greater prognostic significance than the day-14 evaluation. For slow responders on day 7, the day-14 marrow provided additional information. EFS for patients who achieved M1 by day 14 was 65%. EFS decreased to 20% for those still M2 or M3 on day 14. Day-7 and -14 evaluations had significance for patients of all ages and WBC levels. CONCLUSION Marrow aspiration on day 7 of therapy provided more useful information than that on day 14. However, day-14 marrow provided additional information for patients with a poor day-7 response. The rate of cytoreduction is a powerful, independent prognostic factor that can identify patients with a slow early response who are at risk for a short remission duration.
The association between null mutations in the filaggrin gene and contact sensitization to nickel and other chemicals in the general population
Authors: J.P. Thyssen, J.D. Johansen, A. Linneberg, T. Menné, N.H. Nielsen, M. Meldgaard, P.B. Szecsi, S. Stender, B.C. Carlsen
Journal: British Journal of Dermatology (2010)

Journal Article The association between null mutations in the filaggrin gene and contact sensitization to nickel and other chemicals in the general population Get access J.P. Thyssen, J.P. Thyssen National Allergy Research Centre, Department of Dermato‐Allergology, Copenhagen University Hospital Gentofte, University of Copenhagen, Niels Andersens Vej 65, 2900 Hellerup, Denmark Jacob Pontoppidan Thyssen. E‐mail: jacpth01@geh.regionh.dk Search for other works by this author on: Oxford Academic Google Scholar J.D. Johansen, J.D. Johansen National Allergy Research Centre, Department of Dermato‐Allergology, Copenhagen University Hospital Gentofte, University of Copenhagen, Niels Andersens Vej 65, 2900 Hellerup, Denmark Search for other works by this author on: Oxford Academic Google Scholar A. Linneberg, A. Linneberg Research Centre for Prevention and Health, Glostrup Hospital, University of Copenhagen, Copenhagen, Denmark Search for other works by this author on: Oxford Academic Google Scholar T. Menné, T. Menné National Allergy Research Centre, Department of Dermato‐Allergology, Copenhagen University Hospital Gentofte, University of Copenhagen, Niels Andersens Vej 65, 2900 Hellerup, Denmark Search for other works by this author on: Oxford Academic Google Scholar N.H. Nielsen, N.H. Nielsen Dermatology Clinic, Bagsværd, Denmark Search for other works by this author on: Oxford Academic Google Scholar M. Meldgaard, M. Meldgaard Department of Clinical Biochemistry, Copenhagen University Hospital Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark Search for other works by this author on: Oxford Academic Google Scholar P.B. Szecsi, P.B. Szecsi Department of Clinical Biochemistry, Copenhagen University Hospital Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark Search for other works by this author on: Oxford Academic Google Scholar S. Stender, S. Stender Department of Clinical Biochemistry, Copenhagen University Hospital Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark Search for other works by this author on: Oxford Academic Google Scholar B.C. Carlsen B.C. Carlsen National Allergy Research Centre, Department of Dermato‐Allergology, Copenhagen University Hospital Gentofte, University of Copenhagen, Niels Andersens Vej 65, 2900 Hellerup, Denmark Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 162, Issue 6, 1 June 2010, Pages 1278–1285, https://doi.org/10.1111/j.1365-2133.2010.09708.x Published: 01 June 2010
Does Transendocardial Injection of Mesenchymal Stem Cells Improve Myocardial Function Locally or Globally?
Authors: Viky Y. Suncion, Eduard Ghersin, Joel E. Fishman, Juan Pablo Zambrano, Vasileios Karantalis, Nicole Mandel, Katarina H. Nelson, Gary Gerstenblith, Darcy L. DiFede Velazquez, Elayne Breton, Kranthi Sitammagari, Ivonne H. Schulman, Sabrina N. Taldone, Adam R. Williams, Cristina Sanina, Peter V. Johnston, Jeffrey Brinker, Peter Altman, Muzammil Mushtaq, Barry Trachtenberg, Adam M. Mendizabal, Melissa Tracy, Jose Da Silva, Ian K. McNiece, Alberto C. Lardo, Richard T. George, Joshua M. Hare, Alan W. Heldman
Journal: Circulation Research (2014)

Transendocardial stem cell injection (TESI) with mesenchymal stem cells improves remodeling in chronic ischemic cardiomyopathy, but the effect of the injection site remains unknown.To address whether TESI exerts its effects at the site of injection only or also in remote areas, we hypothesized that segmental myocardial scar and segmental ejection fraction improve to a greater extent in injected than in noninjected segments.Biplane ventriculographic and endocardial tracings were recorded. TESI was guided to 10 sites in infarct-border zones. Sites were mapped according to the 17-myocardial segment model. As a result, 510 segments were analyzed in 30 patients before and 13 months after TESI. Segmental early enhancement defect (a measure of scar size) was reduced by TESI in both injected (-43.7 ± 4.4%; n=95; P<0.01) and noninjected segments (-25.1 ± 7.8%; n=148; P<0.001; between-group comparison P<0.05). Conversely, segmental ejection fraction (a measure of contractile performance) improved in injected scar segments (19.9 ± 3.3-26.3 ± 3.5%; P=0.003) but not in noninjected scar segments (21.3 ± 2.6-23.5 ± 3.2%; P=0.20; between-group comparison P<0.05). Furthermore, segmental ejection fraction in injected scar segments improved to a greater degree in patients with baseline segmental ejection fraction <20% (12.1 ± 1.2-19.9 ± 2.7%; n=18; P=0.003), versus <20% (31.7 ± 3.4-35.5 ± 3.3%; n=12; P=0.33, between-group comparison P<0.0001).These findings illustrate a dichotomy in regional responses to TESI. Although scar size reduction was evident in all scar segments, scar size reduction and ventricular functional responses preferentially occurred at the sites of TESI versus non-TESI sites. Furthermore, improvement was greatest when segmental left ventricular dysfunction was severe.
Abstract 3696: Decreased Number Of Circulating Progenitor Cells In Obesity -Beneficial Effects Of Weight Reduction
Authors: Jochen Muller-Ehmsen, Daniela Braun, Thorsten Schneider, Roman Pfister, Franziska Grundmann, Christof Scheid, Markus Flesch
Journal: Circulation (2007)

Aims: Cardiovascular risk factors are associated with decreased levels of circulating progenitor cells (CPC). The aim of this study was to determine whether the number of CPC is an independent correlate of Body-Mass-Index (BMI) and whether weight loss leads to an increase of CPC. Methods: CD34 positive and KDR/CD34, CD133/CD34 and CD117/CD34 double positive cells were measured by FACS in peripheral blood of 149 volunteers (52.5±0.1 years, BMI 21.5–52.7 kg/m 2 , mean 31.6±0.03 kg/m 2 ) participating in a weight reduction program offered by German pharmacies. In addition, carotid intima media thickness and brachial artery flow mediated dilatation were determined. After a diet and sports program for 6 months, 86 representing subjects were re-evaluated (mean weight loss 5.8±0.1 kg). Results: There was an inverse correlation between BMI and CPC. For subjects with BMI&gt;34.1 compared to BMI&lt;28.1 (4 th vs. 1 st quartile) cell counts were: CD34+: 137.7±1.6 vs. 187.0±1.8; KDR/CD34: 17.3±0.7 vs. 55.8±2.0; CD133/CD34: 70.2±1.6 vs. 129.2±1.7; CD117/CD34: 93.1±2.1 vs. 142.7±1.7, all per million leucocytes; p&lt; 0.01 for all parameters). The decrease in CPC in obesity held true not only for the absolute cell numbers, but also for the relative fractions of KDR, CD133 and CD117 positive cells within the CD34 + cells, indicating a specific down regulation of these progenitor cell types. Intima media thickness increased in dependence from BMI (p&lt; 0.001) and was inversely correlated with the number of circulating CD34 positive cell (p&lt; 0.05). After diet, there was a significant increase of CD34 and CD117/CD34 positive cells in subjects whose reduction in BMI was greater than 2.5 kg/m 2 (4 th quartile), which was accompanied by a decrease in intima media thickness (p&lt; 0.05) and an improvement in forearm dilatory response (p &lt; 0.01). Increase in the number of CPC was independent from whether weight loss was achieved by increased physical exercise or by reduced calory intake only. Conclusions: Obesity is associated with decreased numbers of CPC, increased intima media thickness and impaired endothelial function. Diet and weight loss lead to an increase in circulating progenitor cell count, which might contribute to regression of intima media thickness and improved endothelial function.
Inhibition by 3-Methylcholanthrene of Interferon Formation in Rat-Embryo Cells Infected With Sindbis Virus2
Authors: Edward de Maeyer, Jaqueline de Maeyer-Guignard
Journal: JNCI Journal of the National Cancer Institute (1964)

3-Methylcholanthrene (3-MCA), at concentrations of either 1 or 0.1 μg per ml in rat-embryo cell cultures, did not affect cell growth. When complete monolayers were obtained, the cultures were inoculated with Sindbis virus. The yield of infectious virus in the carcinogen-treated cultures was in general somewhat higher than that of control cultures. Interferon yields, however, were markedly decreased, the effect being most pronounced in cultures containing 1 μg per ml of the carcinogen. The same concentrations of 3-MCA also did not influence the viral-inhibitory activity of preformed interferon. This indicated that the carcinogen inhibited the production but not the activity of interferon. Sindbis virus plaques in rat-embryo cell cultures were significantly larger if the cultures had previously been exposed for 24 hours to 1 and 0.1 μg per ml of 3-MCA. This result provided additional, though indirect, evidence for an inhibition of interferon synthesis.
The use of dried cerebrospinal fluid filter paper spots as a substrate for PCR diagnosis of the aetiology of bacterial meningitis in the Lao PDR
Authors: I. Elliott, S. Dittrich, D. Paris, A. Sengduanphachanh, P. Phoumin, P.N. Newton
Journal: Clinical Microbiology and Infection (2013)

We investigated whether dried cerebrospinal fluid (CSF) conserved on filter paper can be used as a substrate for accurate PCR diagnosis of important causes of bacterial meningitis in the Lao PDR. Using mock CSF, we investigated and optimized filter paper varieties, paper punch sizes, elution volumes and quantities of DNA template to achieve sensitive and reliable detection of bacterial DNA from filter paper specimens. FTA Elute Micro CardTM (Whatman, Maidstone, UK) was the most sensitive, consistent and practical variety of filter paper. Following optimization, the lower limit of detection for Streptococcus pneumoniae from dried mock CSF spots was 14 genomic equivalents (GE)/μL (interquartile range 5.5 GE/μL) or 230 (IQR 65) colony forming units/mL. A prospective clinical evaluation for S. pneumoniae, S. suis and Neisseria meningitidis was performed. Culture and PCR performed on fresh liquid CSF from patients admitted with a clinical diagnosis of meningitis (n = 73) were compared with results derived from dried CSF spots. Four of five fresh PCR-positive CSF samples also tested PCR positive from dried CSF spots, with one patient under the limit of detection. In a retrospective study of S. pneumoniae samples (n = 20), the median (IQR; range) CSF S. pneumoniae bacterial load was 1.1 × 104 GE/μL (1.2 × 105; 1 to ×.1 × 106 DNA GE/μL). Utilizing the optimized methodology, we estimate an extrapolated sensitivity of 90%, based on the range of CSF genome counts found in Laos. Dried CSF filter paper spots could potentially help us to better understand the epidemiology of bacterial meningitis in resource-poor settings and guide empirical treatments and vaccination policies.
Phase II Evaluation of Preoperative Chemoradiation and Postoperative Adjuvant Chemotherapy for Squamous Cell and Adenocarcinoma of the Esophagus
Authors: Elisabeth I. Heath, Barbara A. Burtness, Richard F. Heitmiller, Ronald Salem, Lawrence Kleinberg, Jonathan P. S. Knisely, Stephen C. Yang, Mark A. Talamini, Howard S. Kaufman, Marcia I. Canto, Mark Topazian, Tsung-Teh Wu, Koleoso Olukayode, Arlene A. Forastiere
Journal: Journal of Clinical Oncology (2000)

This phase II trial evaluated continuous-infusion cisplatin and fluorouracil (5-FU) with radiotherapy followed by esophagectomy. The objectives of this trial were to determine the complete pathologic response rate, survival rate, toxicity, pattern of failure, and feasibility of administering adjuvant chemotherapy in patients with resectable cancer of the esophagus treated with preoperative chemoradiation.Patients were staged using computed tomography, endoscopic ultrasound, and laparoscopy. The preoperative treatment plan consisted of continuous intravenous infusion of cisplatin and 5-FU and a total dose of 44 Gy of radiation. Esophagogastrectomy was planned for approximately 4 weeks after the completion of chemoradiotherapy. Paclitaxel and cisplatin were administered as postoperative adjuvant therapy.Forty-two patients were enrolled onto the trial. Of the 39 patients who proceeded to surgery, 29 responded to preoperative treatment: 11 achieved pathologic complete response (CR) and 18 achieved a lower posttreatment stage. Five patients had no change in stage, whereas eight had progressive disease (four with distant metastases and four with increases in the T and N stages). At a median follow-up of 30.2 months, the median survival time has not been reached and the 2-year survival rate is 62%. The median survival of pathologic complete responders has not been reached, whereas the 2-year survival rate of this group is 91% compared with 51% in patients with complete tumor resection with residual tumor (P =.03).An excellent survival rate, comparable to that of our prior preoperative trial, was achieved with lower doses of preoperative cisplatin and 5-FU concurrent with radiotherapy.
Oxygen pulse slope analysis during exercise testing identifies patients with systemic sclerosis at a possible risk for developing pulmonary vasculopathy
Authors: Maarten Ninaber, Willem Hamersma, Annemie Schuerwegh, Gabor Kovacs, Horst Olschewski, Jan Stolk
Journal: European Respiratory Journal (2013)

Background:  Patients with systemic sclerosis (SSc) are at risk for developing obliteration of microvascular structures leading to pulmonary hypertension (PH) which warrants early detection.  Methods:  In an observational, prospective design, we studied 121 eligible patients with SSc in an assessment program including non-invasive CPET to analyse an abnormal V’O2/HR slope represented by a breakpoint in the slope. To study the pathophysiologic meaning of such a breakpoint we applied our method to a published data set of a similar population in which CPET and pulmonary arterial pressures were measured simultaneously.  Results:  A pathological V’O2/HR slope was observed in 41 of 121 (34%) patients. Their mean V’O2/HR slope was 6 ± 2 ml, of whom 27 patients (66%) had a normal echocardiographic pulmonary arterial systolic pressures (PASP) at rest. In a historic CPET data set we calculated a breakpoint in both the V’O2/HR slope and V9 O2/mPAP slope in 16 of 45 SSc patients (36%). For each patient we calculated the difference in V’O2 between the two breakpoints. Mean difference in V’O2 was 127 ml ± 63 ml. In 15 patients, a breakpoint in the V’O2/mPAP slope occurred at a lower V’O2 than in the V’O2/HR slope.  Conclusions:  The oxygen pulse breakpoint analysis in non-invasive CPET is a mathematical tool to detect abnormal cardiopulmonary vascular responses to exercise. The breakpoint in the V’O2/mPAP slope occurred at a slightly lower V’O2, suggesting that a sudden increase in pulmonary arterial pressures results in a disproportional increase in heart rate during CPET.
Skin-derived neural precursors competitively generate functional myelin in adult demyelinated mice
Authors: Sabah Mozafari, Cecilia Laterza, Delphine Roussel, Corinne Bachelin, Antoine Marteyn, Cyrille Deboux, Gianvito Martino, Anne Baron-Van Evercooren
Journal: Journal of Clinical Investigation (2015)

Induced pluripotent stem cell–derived (iPS-derived) neural precursor cells may represent the ideal autologous cell source for cell-based therapy to promote remyelination and neuroprotection in myelin diseases. So far, the therapeutic potential of reprogrammed cells has been evaluated in neonatal demyelinating models. However, the repair efficacy and safety of these cells has not been well addressed in the demyelinated adult CNS, which has decreased cell plasticity and scarring. Moreover, it is not clear if these induced pluripotent–derived cells have the same reparative capacity as physiologically committed CNS-derived precursors. Here, we performed a side-by-side comparison of CNS-derived and skin-derived neural precursors in culture and following engraftment in murine models of adult spinal cord demyelination. Grafted induced neural precursors exhibited a high capacity for survival, safe integration, migration, and timely differentiation into mature bona fide oligodendrocytes. Moreover, grafted skin–derived neural precursors generated compact myelin around host axons and restored nodes of Ranvier and conduction velocity as efficiently as CNS-derived precursors while outcompeting endogenous cells. Together, these results provide important insights into the biology of reprogrammed cells in adult demyelinating conditions and support use of these cells for regenerative biomedicine of myelin diseases that affect the adult CNS.
The Predictive Value of Bone Morphogenetic Protein 9 (Bmp-9) in the Serum of Hcc Patients Treated with Sorafenib
Authors: L. Chelis, K. Anagnostopoulos, S. Vradelis, N. Xenidis, K. Amarantidis, D. Pitsiava, P. Michailidis, E. Christakidis, A. Chalvatzis, D. Matthaios, I. Biziota, A. Kortsaris, S. Kakolyris
Journal: Annals of Oncology (2014)
Evaluation of peak flow meter for detecting airflow limitation and predicting clinical diagnosis
Authors: Yogesh Thorat, Shweta Rasam, Sundeep Salvi, Rahul Kodgule, Rahul Ramesh
Journal: European Respiratory Journal (2014)

Background:  Peak flow meter (PFM) is a screening tool for asthma and COPD. The ability of PFM to detect airway obstruction compared to standard spirometer is inadequately studied.   Methods:  PEF measurement (Breathometer, Cipla ltd.) and spirometry (Koko) were conducted in 111 subjects (asthma=69, COPD=18, healthy=24) by a well trained technician. Sensitivity and specificity of peak flow meter to detect airway obstruction were studied against spirometry and final clinical diagnosis and cut-off for %predicted was obtained by receiver operating characteristic analysis.   Results:  Compared to standard spirometry, PFM had sensitivities of 91% and 75% and specificities of 77% and 91% at cut-offs of 80% predicted and 75% predicted respectively to detect airflow limitation. For predicting confirmed clinical diagnosis of healthy and airway obstruction (asthma/COPD) sensitivity and specificity of standard spirometry and PFM were 83% and 70%; and 80% and 85% with cut-offs of 0.7 and 50% predicted respectively  . An increase of 60 L/min in PEF had sensitivity of 59% and specificity of 80% to detect reversibility on spirometry.   Conclusion:  Peak expiratory flow
Screening for biomarkers in pulmonary hypertension
Authors: Svenja Lena Tiede, Soni Savai Pullamsetti, Akylbek Sydykov, Günter Lochnit, Henning Tiede, Hossein Ardeschir Ghofrani, Norbert Weissmann, Friedrich Grimminger, Werner Seeger, Ralph Theo Schermuly
Journal: European Respiratory Journal (2012)

Introduction: Pulmonary hypertension (PH) is a progressive and fatal disease. The gold standard for diagnosing PH and estimating prognosis is the invasive method of right heart catheterization. To date no biomarker is available to prove or exclude the diagnosis of PH.  Aims and objectives: The aim of this study is to identify and validate new biomarkers for PH.  Methods: Plasma from the pulmonary artery banding (PAB) and the monocrotaline (MCT) rat model, and corresponding sham and control animals (n=9), was used for 2D-gel electrophoresis (2D-GE) and MALDI-TOF-MS analysis. Further, plasma changes of interesting candidates were confirmed by ELISA. Human study population consists of patients with idiopathic pulmonary arterial hypertension (n=40), PH associated with collagen vascular disease (n=45), pulmonary venous hypertension (n=44), chronic thromboembolic PH (n=45), and non-PH controls (n=34).  Results: The spot density analysis of 2D-GE and identification by MALDI-TOF-MS revealed 7 proteins significantly changed in PAB vs. sham, and 15 proteins in MCT vs. control group. Complement component 4 (C4) and complement inhibitory factor H (CFH) were upregulated in PAB and MCT. ApoE was changed 15-fold in MCT plasma, but not in PAB. The analysis of the human samples revealed no significant difference in mean plasma ApoE between the patient groups (119.4±10.3, 147.6±11.6, 116.8±9.9, 110.2±8.3 µg/ml) and controls (135.3±14 µg/ml).  Conclusions: Despite published data on the role of ApoE in PH and the significant changes in rats, ApoE seems not suitable as biomarker for PH in humans. Other candidates identified by mass spectrometry will be evaluated for their potential as biomarker.