tbuckley/cabot-search huggingface dataset:
Abstract 555: DNA methylation patterns are associated with subpopulations of diffuse large B-cell lymphoma
Authors: Jenny Wu, Ron McCord, Thomas Sandmann, Kim Walter, Richard Bourgon, Robert Soriano, Zora Modrusan, Walter Darbonne, Kirsten E. Mundt
Journal: Cancer Research (2014)

Abstract Diffuse large B-cell lymphoma (DLBCL) comprises two predominant molecular subtypes: germinal-center B-cell-like (GCB) and activated B-cell-like (ABC). These subtypes were initially identified by their gene expression profiles, which are reminiscent of the cell of origin of the lymphoma. Though histologically indistinguishable, these subtypes are associated with distinct clinical outcomes and may be predictive for response to novel therapies. The development of improved diagnostic biomarkers for these DLBCL subtypes could therefore inform clinical decision-making by enabling selection of patients likely to respond to such novel therapies. To investigate regulatory mechanisms potentially responsible for the differential gene expression patterns seen in the DLBCL subtypes and to identify potential biomarkers for these subtypes, we performed whole genome methylation profiling of 15 DLBCL cell lines and 21 DLBCL patient tissues and identified distinct DNA methylation patterns associated with the two DLBCL molecular subtypes. Analysis of the methylation patterns of several DLBCL classifier genes known to be differentially expressed in ABC compared to GCB revealed the classic DNA methylation correlation pattern: high mRNA expression was associated with low methylation in the vicinity of the transcriptional start site (TSS), and vice versa. Furthermore, comparison of differential methylation profiles with corresponding RNA-seq data further supports the notion that DNA methylation profiles underlie the gene expression patterns that are currently used to classify ABC and GCB DLBCL subtypes. Collectively, these data suggest that DNA methylation may contribute to the regulation of expression of key ABC and GCB DLBCL classifier genes and further, that the DNA methylation state at these loci could potentially serve as useful diagnostic biomarkers for the DLBCL subtypes. Citation Format: Jenny Wu, Ron McCord, Thomas Sandmann, Kim Walter, Richard Bourgon, Robert Soriano, Zora Modrusan, Walter Darbonne, Kirsten E. Mundt. DNA methylation patterns are associated with subpopulations of diffuse large B-cell lymphoma. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 555. doi:10.1158/1538-7445.AM2014-555
THE PREVALENCE OF ANTIBIOTICALLY TREATED PEOPLE
Authors: J R KINNEY
Journal: Annals of Internal Medicine (1957)

Article1 July 1957THE PREVALENCE OF ANTIBIOTICALLY TREATED PEOPLEJANET R. KINNEY, M.D.JANET R. KINNEY, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-47-1-71 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe problems of antibiotic treatment, reactions and resistance become more important each day as the number of patients who have had previous treatment increases. Since the incidence of these problems in an average population group is largely unknown, a study of these factors was undertaken.The community studied was the nursing staff of the Cook County Hospital. This group is basically in good health, since all members are able to carry on their profession. It varies from the general population in that it is predominantly female and young. It has the advantage for this study that most of its members...Bibliography1. Dowling HF: Antibiotic therapy, DM 25 (Mar.) 1956. Google Scholar2. Lowell FC: Allergy to sulfonamide and antibiotic drugs, Ann. Int. Med. 43: 335 (Aug.) 1955. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Chicago, Illinois*Received for publication August 10, 1956.From the Department of Medicine, University of Illinois.Requests for reprints should be addressed to Janet R. Kinney, M.D., 224 South Michigan Avenue, Chicago 4, Illinois . PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 July 1957Volume 47, Issue 1Page: 71-77KeywordsAdverse reactionsAllergy and immunologyAntibiotic resistanceAntibioticsEndocarditisGlomerulonephritisSepsisStreptococcal infections ePublished: 1 December 2008 Issue Published: 1 July 1957 PDF downloadLoading ...
Hyperdiploidy in Childhood AML Associated with Low Age and AML-M7. A NOPHO-AML Study and Literature Review,
Authors: Julie Damgaard Sandahl, Jonas Abrahamsson, Jesper Heldrup, Kirsi Jahnukainen, Olafur G Jonsson, Birgitte Lausen, Josefine Palle, Bernward Zeller, Erik Forestier, Eigil Kjeldsen, Henrik Hasle
Journal: Blood (2011)
Cannabinoids might reduce spasticity in multiple sclerosis
Authors: Scott Gottlieb
Journal: BMJ (2000)

Cannabinoids, the active ingredients found in marijuana, may help control the tremors and muscle spasticity experienced by patients with multiple sclerosis, says a new study (Nature 2000;404:84-7).

Researchers examined mice with chronic relapsing experimental allergic encephalo-myelitis, an autoimmune disease which has symptoms considered to be closely related to those of multiple sclerosis. …
Skin Electronics: Oxide Nanomembrane Hybrids with Enhanced Mechano‐ and Thermo‐Sensitivity for Semitransparent Epidermal Electronics (Adv. Healthcare Mater. 7/2015)
Authors: Minjoon Park, Kyungsik Do, Jaemin Kim, Donghee Son, Ja Hoon Koo, Jinkyung Park, Jun‐Kyul Song, Ji Hoon Kim, Minbaek Lee, Taeghwan Hyeon, Dae‐Hyeong Kim
Journal: Advanced Healthcare Materials (2015)

Wearable mechanical and temperature sensors based on oxide nanomembrane hybrids are developed by D.-H. Kim and co-workers on page 992. Ultrathin and optically invisible materials make wearable devices fully compatible with natural human skin. Nanomaterials embedded in oxide nanomembranes dramatically enhance the mechano- and thermosensitivity, which enables successful demonstrations of the wheelchair control and hypothermia detection.
Inhibition of β-catenin signaling in human colon cancer cells and ApcMin mice by (-)-Epigallocatechin-3-gallate
Authors: Jihyeung Ju, Jungil Hong, Xinpei Hao, Yingying Liu, Chung S. Yang
Journal: Cancer Research (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

4889 

Previously, we reported that oral administration of (-)-epigallocatechin-3-gallate (EGCG), a major catechin in green tea, not only inhibited small intestinal tumor formation in Apc Min mice but also resulted in decreases of nuclear β-catenin and c-Myc levels and increases of E-cadherin levels in small intestinal tumors (Cancer Res, 2005; 65(22): in press) . The purpose of present study was to investigate mechanisms involved in the inhibition of aberrant β-catenin signaling by EGCG in vitro and in vivo . In HCT116 human colon cancer cells, treatment with EGCG at concentrations of 20, 50, and 100 μM decreased the levels of nuclear β-catenin but increased the levels of E-cadherin, which is a similar finding to our previous results in another human colon cancer cell line, HT29 (Cancer Res, 2005; 65(22): in press) . The EGCG treatment also suppressed important upstream events for the release of β-catenin from plasma membrane to cytoplasm and the degradation of E-cadherin; levels of tyrosine phosphorylation of β-catenin and p120ctn as well as the phosphorylation (Thr 12) of Fyn were decreased under the EGCG treatment. In studies with 14-week old Apc Min/+ mice, small intestinal tumors were found to have higher levels of IGF-1R and Fyn than normal small intestine tissues. In both normal and tumor tissues from Apc Min/+ mice that were given intragastric administration of EGCG (75 mg/kg) 3 h before sacrifice, phosphorylation levels of IGF-1R, Fyn, β-catenin, and p120ctn were decreased as compared to the levels in the normal and tumor tissues from control mice. These results suggest that EGCG suppresses abberant β-catenin signaling possibly through inhibition of phosphorylation of β-catenin, p120ctn, IGF-1R, and Fyn, and these effects may contribute to the inhibition of tumorigenesis in Apc Min mice (supported by NIH grant CA 88961) .
Abstract 17940: PDEV Inhibition + BNP Administration Enhanced the Cardiac but not the Renal Response to Acute Volume Overload in Human Preclinical Diastolic Dysfunction as Compared to PDEV Inhibition Alone
Authors: Isabel Torres-Courchoud, Paul M Mckie, Sherry Benike, John A Schirger, Margaret M Redfield, John C Burnett, Horng H Chen
Journal: Circulation (2014)

Background: Preclinical diastolic dysfunction (PDD) is defined as subjects with normal systolic function, moderate or severe diastolic dysfunction determined by Doppler criteria but with no symptoms of heart failure (HF). We have previously demonstrated that an impaired cardiorenal endocrine response to stress by acute volume loading (AVL) exists in PDD and that is corrected by acute administration of sq B-type natriuretic peptide (BNP). Type V phosphodiesterase (PDEV) metabolizes cGMP, second messenger of BNP. The objective of the current study is to determine if the combination of PDEV inhibitor (PDEVI) and BNP will result in a greater enhancement of the cardiorenal response to AVL as compared to PDEVI alone in PDD. Methods: Randomized double-blind single placebo-controlled cross-over study in 20 PSD subjects. We determined cardiorenal effects of PDEVI with Tadalafil (T) 5 mg alone and in combination with acute sq BNP (Nesiritide 10 μg/Kg), in response to AVL (normal saline 0.25 ml/kg/min for 1 hour). Each subject had 2 study visits, separated by at least 1 week. At each visit, echocardiogram and renal clearance studies with iothalmate and PAH to measure GFR and renal plasma flow (RPF) were done before and after AVL. Each patient was randomized to receive T at both visits and either placebo or sq-BNP one visit and crossover to the other on the next visit. Results: T+BNP resulted in smaller LA volume index (-4±10 vs 3±7 ml/m 2 , p&lt; 0.05) and lower right ventricular systolic pressure (-4±3 vs 2±6 mmHg, p&lt; 0.05) in response to AVL as compared to T alone. Moreover, T+ BNP resulted in a significant reduction of plasma ANP and LV end-systolic volume in response to AVL while T alone did not. However, the combination to T+BNP did not enhance the renal response to AVL as compared to T alone. Both the change in urine flow (1.7±5 vs 2.4±5 ml/min, p&gt; 0.05) and sodium excretion (129±200 vs 124±270 mEq/min, p&gt;0.05) in response to AVL were similar between T+BNP and T alone. Conclusion: In PDD, the combination of PDEVI and BNP reduced LV filling pressure in response to AVL as compared to PDEVI alone. However, it did not enhance the renal response to AVL. This suggests a differential cardiac versus renal response to the maximization of cGMP system with PDEVI and BNP.
Dutch GPs take three day strike action
Authors: Tony Sheldon
Journal: BMJ (2001)

In the Netherlands 7000 GPs went on strike last week for three days—the first such action in their history.

They claim that insufficient government investment in general practice is threatening the quality of their care. An estimated 90% of GPs responded to the strike call from the Dutch Association of General Practitioners (Landelijke Huisartsen Vereniging), after a meeting of representatives voted 725 to 5 for the strike, with 11 abstentions.

Routine operations were cancelled as patients needing to see a doctor arrived at …
Abstract 12636: Pro-Atrial Natriuretic Peptide: A Novel Guanylyl Cyclase-A Receptor Activator Which Goes Beyond Carperitide and Nesiritide
Authors: Tomoko Ichiki, Brenda K Huntley, Ingrid A Andersen, S Jeson Sangaralingham, Gail J Harty, Sharon M Sandberg, Denise M Heublein, John C Burnett
Journal: Circulation (2014)

Introduction: ProANP is a 108 amino acid (AA) prohormone which is produced in the atria and processed to the mature 28 AA ANP which activates the guanylyl cyclase receptor-A (GC-A) and its second messenger cGMP. ProANP is found in the human circulation but its bioavailability is undefined. Hypothesis: ProANP represents a novel cardiac peptide with more sustained natriuretic actions than either carperitide (ANP) or nesiritide (BNP). ProANP itself can activate GC-A in vitro and the proANP can be processed in human serum from normals and in patients with heart failure (HF). Methods: We examined the in vivo cardiorenal actions of proANP compared to ANP, BNP, or placebo in normal canines (667 pmol/kg, n=5/group). We determined cGMP activation of proANP, ANP and BNP in GC-A expressing HEK293 cells. ProANP processing and degradation was also defined in serum from normal subjects (n=13) and patients with HF (n=14) ex vivo. Results: ProANP had significantly greater diuretic and natriuretic properties with increased renal blood flow and sustained renal tubular actions compared to ANP or BNP in canines. ProANP also resulted in greater and more prolonged cardiac unloading than ANP, and much less hypotensive effects than BNP. The plasma half-life of proANP was 4.5 times longer than ANP and 3.8 times longer than BNP. ProANP itself stimulated cGMP generation in GC-A expressing HEK cells which was equal to ANP. ProANP was processed to ANP in fresh serum from normals as well as HF patients ex vivo and the processed peptide activated cGMP in GC-A expressing HEK cells. Conclusions: ProANP represents a novel GC-A activator with a longer half-life and greater natriuresis and diuresis than ANP and BNP and less hypotensive effects than BNP. The bioactivity of exogenous proANP may be through direct stimulation of GC-A, as well as through processing into ANP, as proANP is processed in the circulation of both normal and HF. As proANP circulates in the human circulation, proANP may represent a key circulating hormone in cardiorenal and blood pressure homeostasis as well as a potential innovative therapeutic beyond ANP (carperitide) or BNP (nesiritide) for cardiorenal diseases including HF.
Abstract 3862: Isolation and nanoscale visualization of glioblastoma stem-like cells utilizing the Notch1 receptor
Authors: Elliot Pohlmann, Susan Murphy, Debbie Kelly, Zhi Sheng
Journal: Cancer Research (2014)

Abstract Tumor eradication is an elusive goal in modern medicine. This unmet goal is in part due to the imprecise therapeutic targeting of a specific population of cells having “stem-like” properties. These stem-like tumor cells can remain almost senescent during chemotherapeutic interventions to evade treatment and produce genetically different secondary tumor lineages than their parental tumors. Here we develop a novel approach that utilizes the Notch1 receptor, found on the surface of glioma cells, to isolate a population of cells bearing stem-like properties. We identified this population to be Nestin+GFAP-, capable of serum differentiation into Nestin-GFAP+ cells, and self-regenerating. In addition, this unique population of cells has increased stem-like qualities compared to the CD133 marker. Utilizing affinity nanocapture grids, we were able to visualize Notch1+ stem-like cells and the perforation of their plasma membranes by unlabeled gold nanorods via electron microscopy. Previous studies have shown that tumor stem-like cells can exist within the larger tumor milieu, representing a potential threat to recurrence. We propose that 1) Notch1 may serve as a novel marker to define glioblastoma stem-like cells, capable of being used for targeting chemotherapeutic approaches in the future, and 2) affinity nanocapture grids represent a unique method for examining individual living cells at the nanoscale. Citation Format: Elliot Pohlmann, Susan Murphy, Debbie Kelly, Zhi Sheng. Isolation and nanoscale visualization of glioblastoma stem-like cells utilizing the Notch1 receptor. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3862. doi:10.1158/1538-7445.AM2014-3862
Letter by Fenici et al Regarding Articles, “Wolff-Parkinson-White Syndrome in the Era of Catheter Ablation: Insights From a Registry Study of 2169 Patients” and “The Asymptomatic Wolff-Parkinson-White Patient: Time to be More Proactive?”
Authors: Riccardo Fenici, Donatella Brisinda, Angela Venuti
Journal: Circulation (2015)
Abstract P208: Changes in metabolic profiles among evacuees after the Great East Japan Earthquake: the Fukushima Health Management Survey
Authors: Tetsuya Ohira, Mitsuaki Hosoya, Seiji Yasumura, Hiroaki Sato, Hitoshi Suzuki, Akira Sakai, Akira Ohtsuru, Gen Kobashi, Kotaro Ozasa, Kenji Kamiya, Shunichi Yamashita, Masafumi Abe
Journal: Circulation (2014)

Background: The Great East Japan Earthquake occurred on 11 March 2011 in Japan. To this day, over 140,000 people have been forced to evacuate their homes due to a nuclear accident, which occurred at the Fukushima Daiichi Nuclear Power Plant. Hypothesis: We assessed the hypothesis that prevalence of obesity, hypertension, dyslipidemia, and diabetes mellitus was increased among evacuees after the disaster, compared with that before the disaster. Methods: A prospective study of Japanese subjects aged 40-90 years was undertaken using data collected from 32,949 participants (14,963 men and 17,986 women) in general health checkups conducted in 12 communities, including the evacuation zone specified by the government, between 2008 and 2010. Height, body weight, blood pressure, and blood test such as glucose, hemoglobin A1c (HbA1c), and lipid panel were examined. Follow-up examination was conducted between 2011 and 2012 (at least of 6-month after the disaster). The participants were divided into two groups: evacuees and non-evacuees. Changes in metabolic profiles among the evacuees and non-evacuees were compared between before and after the disaster. Results: 22,117 participants (10,170 men and 11,947 women, follow-up rate: 67%) received follow-up examination after the disaster, and an average follow up was 1.5-years. Mean levels of body weight were significantly increased in the both evacuee and non-evacuee groups after the disaster, and changes in body weight and body mass index were greater in the evacuee group than those in the non-evacuee group; +1.3kg versus +0.3kg, p&lt;0.001, and +0.60kg/m 2 versus +0.13kg/m 2 , p&lt;0.001. In the evacuee group, prevalence of obesity, hypertension, dyslipidemia, and diabetes mellitus was increased after the disaster. Respective prevalence before and after the disaster were 31.8% and 39.4% for obesity (body mass index&gt;=25.0kg/m 2 ), 54.2% and 60.2% for hypertension, 41.2% and 53.9% for dyslipidemia, and 10.2% and 11.9% for diabetes mellitus, while, in the non-evacuee group, those were 28.3% and 30.3%, 54.7% and 60.0%, 42.6% and 49.4%, and 8.5% and 9.9%. Conclusions: Prevalence of obesity, hypertension, dyslipidemia, and diabetes mellitus may be increased among residents, especially evacuees, in the evacuation zone of Fukushima prefecture after the Great East Japan Earthquake.
Abstract 16141: Predictors of Death in Patients With Chagas Cardiomyopathy and Pacemaker - Preliminary Results of the PACINCHAGAS Study
Authors: Giselle L Peixoto, Rodrigo O Madia, Sérgio F Siqueira, Mariana M Lensi, Silvana D Nishioka, Anísio Pedrosa, Ricardo A Teixeira, Roberto Costa, Martino Martinelli
Journal: Circulation (2014)

Introduction: Chagas’ disease causes different clinical expressions in the heart and permanent pacing due to bradycardia is not uncommon. Objective: Predictors of death in patients with Chagas Cardiomyopathy requiring pacemaker (PM) are unknown. This is the aim of this study. Methods: We prospectively evaluated 529 patients included in the Pacinchagas Study - Risk Stratification in Pacemaker Patients with Chagas Cardiomyopathy, which primary objective is to create a risk score to predict death in this population. The patients are submitted to an extent questionnaire which included clinical (NYHA class, symptoms, comorbidities and medications) functional (electrocardiography, Holter and echocardiography) and electronic variables (burden of pacing and arrhythmias). Patients with at least 6 months of follow-up were included in this preliminar analysis. Results: The cohort included 337 (63.7%) females, the mean age was 62.3±11.9 years and 63.1% were in NYHA class I. Indication for PM implantation was atrioventricular block, sick sinus syndrome, atrial fibrillation with slow ventricular response and unknown in 72.0%; 20.4%; 5.1% and 2.5%, respectively. During a mean follow-up of 1.5±0.6 years, 62 (11.7%) patients died. The mean time of PM implantation was not different between the dead and the survivors (11.9±9.0years versus 11.1±8.6years, P=0.503). Twenty-five deaths (40.3%) were sudden, 22 (35.5%) were due to heart failure, 6 (9.7%) were due to other cardiovascular causes, and 7 (11.3%) were due to noncardiovascular causes. The cause of death could not be determined in two patients (3.2%). Cox proportional hazards identified three predictors of death: NYHA class III/IV (Hazard Ratio [HR] 5.661; 95% Confidence Interval [95%IC] 2.617-12.245; P&lt;0.001); left ventricular ejection fraction (LVEF) ≤42% (HR 2.779; 95%IC 1.299-5.945; P=0.008) and chronic kidney disease (HR 2.635; 95%IC 1.167-5.948; P=0.020). Conclusions: This analysis of Pacinchagas study identified in a mean follow-up of one year and half, three predictors of death in PM users with Chagas Cardiomyopathy: NYHA class III/IV, LVEF≤42% and chronic kidney disease.
Use of caffeine to lengthen seizures in ECT
Authors: P E Hinkle, C E Coffey, R D Weiner, M Cress, C Christison
Journal: American Journal of Psychiatry (1987)

During a course of ECT, seizure duration may become too brief for clinical benefit. Use of higher-energy stimuli may lengthen seizures but may also increase the risk of toxicity, and it is not possible when maximum settings are reached. The authors present the cases of six drug-free depressed inpatients whose seizure durations in ECT declined despite maximum settings on three different ECT devices. In all cases, pretreatment with caffeine lengthened seizures (mean increase = 107%), and clinical improvement followed. Caffeine was well tolerated, even in patients with cardiovascular diseases.