tbuckley/cabot-search huggingface dataset:
Abstract 518: Kisspeptin stimulates invasiveness of ERα -negative human mammary epithelial and breast cancer cells.
Authors: Moshmi M. Bhattacharya, Dragana Cvetkovic, Magdalena Dragan, Hon Sing Leong, Andy Babwah
Journal: Cancer Research (2013)

Abstract Recently, we have shown that KP-10 stimulates invasion of estrogen receptor (ERα)-negative breast cancer cells via transactivation of the epidermal growth factor receptor (EGFR) [Zajac et al. (2011) PLoS ONE 6(6): e21599]. Here we report that KP-10 stimulated cell motility and invasiveness of ERα-negative non-malignant mammary epithelial MCF10A cells that endogenously expressing KISS1R, and MCF10A cells stably expressing KISS1R using three-dimensional (3D) Matrigel cultures. Additionally, exogenous expression of KISS1R in ERα-negative SKBR3 breast cancer cells stimulated invasion in 3D cultures and induced extravasation in vivo using chorioallantoic membrane (CAM) assays. Exogenous KISS1R expression in MCF10A and SKBR3 cells also induced a partial epithelial-to-mesenchymal transition like phenotype as evidenced by the acquisition of a spindle-shaped morphology, intracellular localization of the epithelial marker E-cadherin, increased stress fibre formation and elevated levels of the mesenchymal markers, Snail/Slug, vimentin and N-cadherin. In contrast, KP-10 had no effect on migration and invasion of the ERα-positive T47D and MCF7 breast cancer cells and failed to transactivate EGFR in the ERα-positive cells. This suggested that ERα negatively regulates KISS1R-dependent breast cancer cell migration, invasion and EGFR transactivation. In support of this, we found KP-10-stimulated cell migration, invasion and EGFR transactivation were ablated upon stably expressing ERα in the ERα-negative MDA-MB-231 cells. Lastly, we found that KISS1R was localized at the leading edge of motile cells, where it co-localized with the actin scaffolding protein, IQGAP1. We have identified IQGAP1 as a novel binding partner of KISS1R and have demonstrated that KISS1R regulates EGFR transactivation in breast cancer cells in an IQGAP1-dependent manner. Overall, our data strongly suggest that the ERα status of mammary cells will dictate whether the KISS1R signaling pathway may be a novel clinical target for the treatment of breast cancer metastasis. Citation Format: Moshmi M. Bhattacharya, Dragana Cvetkovic, Magdalena Dragan, Hon Sing Leong, Andy Babwah. Kisspeptin stimulates invasiveness of ERα -negative human mammary epithelial and breast cancer cells. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 518. doi:10.1158/1538-7445.AM2013-518
Nicotinic Acid in NIDDM
Authors: K. G. M. M. Alberti
Journal: JAMA (1990)

<h3>To the Editor.—</h3> We read with interest the article by Garg and Grundy<sup>1</sup>in which they convincingly confirmed that nicotinic acid therapy, while greatly improving the lipid profile in patients with NIDDM, caused a marked deterioration in glycemic control. The authors conclude that nicotinic acid should not be used as first-line therapy in patients with NIDDM. The authors suggest that nicotinic acid may cause insulin resistance or enhance hepatic utilization of fatty acids, thereby promoting gluconeogenesis. We would like to suggest that the hyperglycemic effects of nicotinic acid are due to increased circulating nonesterified fatty acid (NEFA) levels caused by the pharmacodynamic effects of the drug. Nicotinic acid has a short suppressive effect on lipolysis and early studies clearly showed that after an early drop in NEFA levels, there was a marked rebound.<sup>2</sup>Glucose levels closely parallel NEFA levels, the NEFAs acting presumably through impaired peripheral glucose oxidation—the Randle
Robbie's Fox cards.
Authors: J. Howie
Journal: BMJ (1989)

Patients with Paget's disease affecting the maxilla or mandible are often first detected by dentists as tooth extraction may cause problems and because any artificial dentures will not remain satisfactory for more than a few months.The finding of pagetoid deformity, facies leontina, and hypertrophic clavicles in Metsys's painting questions the belief of Sir James Paget that Paget's disease was a condition that had arisen fairly recently.
Mozart's scatological disorder.
Authors: P J Davies
Journal: BMJ (1993)

practitioners are in a position to plan because they have survived many changes, and to fail to re- appoint them on the basis of a local health policy "whim" conflicts with individual patients' rights in choosing their own general practitioner.If managers were better informed of practice activity before 1990 and spent time equipping general practitioners instead of attacking them, they would learn from the general practitioner how to maintain quality of care.This aspect is being addressed very successfully by the Medical Audit Advisory Groups.
ASH 2010 meeting report—Top 10 clinically oriented abstracts in coagulation medicine and platelet disorders
Authors: Rajiv K. Pruthi
Journal: American Journal of Hematology (2011)

This abstract provides a final report on outcomes of 292 adult ITP patients who were enrolled on romiplostim studies. Of the 292 patients, 32.5% had undergone a splenectomy and romiplostim treatment was administered for a median of 78 weeks (range, 1–277) at a median weekly dose of 4 mcg/kg (2.2–7.3 mcg/kg). The large majority (94.5%) achieved a platelet count ≥50 × 109/L during the study with the target platelet count (50–200 × 109/L) being achieved and maintained after the first week of therapy. Of the patients on concurrent ITP medications prior to enrollment (n = 37), 81% (n = 30) were able to discontinue or reduce the dose by >25%. Mild adverse events included headache (38%), nasopharyngitis (34%), and fatigue (32%). In those that had a bone marrow biopsy, reticulin was present or increased in 11 patients. Neutralizing antibodies to romiplostim were detected in 2 patients; however, there was no cross-reactivity with thrombopoietin and the antibodies cleared after drug withdrawal. Of the 16 deaths, 2 were possibly attributable to treatment (unstable angina, myocardial infarction). During the study period, 25 thromboembolic events were reported.
Early versus Late Feeding of Infants of Diabetic Mothers
Authors: John P. Hubbell, James E. Drorbaugh, Arnold J. Rudolph, Peter A. M. Auld, Ruth B. Cherry, Clement A. Smith
Journal: New England Journal of Medicine (1961)

FOR the past several years the usual plan of feeding of infants of diabetic mothers in the Joslin Clinic group delivered at the Boston Lying-in Hospital has been to start with a glucose-and-water solution on the third day.1 Parenteral feedings were rarely if ever used, and in infants with symptoms of respiratory distress, oral feedings were frequently postponed for seventy-two or even ninety-six hours. However, Reardon et al.2 have advocated earlier feedings starting at two to twelve hours of age and consisting of 0.45 per cent sodium chloride in five per cent glucose solution to be given at a rate . . .
A Single Center Review of the Use of Mycophenolate Mofetil in the Treatment of Autoimmune Hepatitis
Authors: Jonathan T. Hlivko, Mitchell L. Shiffman, R. Todd Stravitz, Velimir A. Luketic, Arun J. Sanyal, Michael Fuchs, Richard K. Sterling
Journal: Clinical Gastroenterology and Hepatology (2008)
Affinity-labeled plasma somatomedin-C/insulinlike growth factor I binding proteins. Evidence of growth hormone dependence and subunit structure.
Authors: J R Wilkins, A J D'Ercole
Journal: Journal of Clinical Investigation (1985)

By using disuccinimidyl suberate, we have covalently cross-linked 125I-labeled somatomedin-C (Sm-C)/insulinlike growth factor I to specific binding proteins in human plasma. In unfractionated plasma samples from normal and acromegalic donors, 125I-Sm-C binding-protein complexes with relative molecular weights (Mr) of 160,000, 135,000, 110,000, 80,000, 50,000, 43,000-35,000, and 28,000-24,000 were consistently observed. In contrast, the 43,000-35,000-mol wt species were frequently the only specific complexes observed in hypopituitary plasma and were consistently more intensely labeled in such samples. Reduction of samples with beta-mercaptoethanol did not alter the electrophoretic pattern of these 125I-Sm-C binding-protein complexes. All Sm-C binding proteins, with the exception of the 43,000-35,000-mol wt complex, were adsorbed by concanavalin A-Sepharose. When acromegalic or normal plasma was fractionated on a Sephadex G-200 column and affinity labeled, the same complexes that were adsorbed by concanavalin A were found in fractions that eluted near the gamma-globulin peak. On the other hand, the 43,000-35,000-mol wt complex consistently eluted in size-appropriate fractions near the albumin peak. These data suggest that the growth hormone (GH)-dependent Sm-C binding protein, represented by the 160,000-mol wt complex, is in some way composed of smaller species, i.e., the 135,000-, 110,000-, 80,000-, 50,000-, and 28,000-24,000-mol wt complexes. Acid incubation of plasma prior to Sephadex G-200 chromatography results in the elimination of specific 125I-Sm-C binding-protein complexes which elute near gamma-globulin and a concurrent increase in the labeling intensity of the 28,000-24,000-mol wt complexes. We speculate, therefore, that each of the GH-dependent Sm-C binding-protein complexes represents an oligomer composed of 28,000-24,000-mol wt protomers. The 43,000-35,000-mol wt species is not dependent upon GH and appears to represent a different type of Sm-C binding protein.
Calculating the Workforce in General Surgery
Authors: Olga Jonasson
Journal: JAMA (1995)

To provide a reasonable estimate of the patient care and resident physician workforce practicing general surgery in 1994.Data regarding general surgical residents and practicing general surgeons were obtained from four sources and compared with previously published numbers.Information was derived from the American College of Surgeons' Longitudinal Study of Surgical Residents: 1992-1993; the American Medical Association's Physician Characteristics and Distribution in the United States, 1994 Edition; the American Board of Medical Specialties' database on general surgeons; and the American Board of Surgery recertification data from the files of diplomates since 1968. Each of these sources was analyzed separately to derive a count of graduates from general surgery residency programs and an estimate of fully trained general surgeons engaged in patient care activities.We found that approximately 600 graduates of general surgery residency programs enter the practice of general surgery each year, and we found a close correlation between maximum and minimum estimates of the number of fully trained general surgeons engaged in active patient care, certified general surgeons who are not retired, and currently certified general surgeons. This number (17,289 to 23,502) is approximately half that commonly used in calculations of the general surgery workforce (38,239). The larger number includes surgeons with subspecialty training beyond general surgery, surgical residents, and surgeons not engaged in patient care.Estimations of the workforce in general surgery and predictions of future needs for general surgeons must be derived from the appropriate number of general surgery residents and practicing general surgeons.
Diagnostic accuracy of the bronchodilator response in children
Authors: Sze Man Tse, Diane R. Gold, Joanne E. Sordillo, Elaine B. Hoffman, Matthew W. Gillman, Sheryl L. Rifas-Shiman, Anne L. Fuhlbrigge, Kelan G. Tantisira, Scott T. Weiss, Augusto A. Litonjua
Journal: Journal of Allergy and Clinical Immunology (2013)
Cytosine arabinoside modulation of the mitotic index in treatment of adult acute leukemia
Authors: Archie A. Mackinney, Bridgett Flynn
Journal: American Journal of Hematology (1978)

Abstract Twenty‐three unselected adult acute leukemic patients were treated with an initial intravenous pulse of cytosine arabinoside (Ara‐C) 5 mg/kg. The mean mitotic index dropped from 7.7/1,000 to 2.8/1,000 at 4 hours after Ara‐C pulse and rebounded to 6.7/1,000 at 18 hours. Infusions of Ara‐C 7‐10 mg/kg were given for 9 or 12 hours, depending on changes in the mitotic index. The group of responders (M 1 or M 2 marrows) could be predicted by the initial blast count in the marrow and the change in the mitotic index 18 hours after the Ara‐C pulse. There were 9 (39%) complete and partial remissions. Six patients remained in remission more than 200 days. Since the changes in the mitotic indices induced by Ara‐C were indistinguishable from those of another more successful trial, we infer that factors other than modulation of the cell cycle are critical to the success of this mode of therapy.
NOUVELLES RECHERCHES ELECTRO‐ENCEPHALOGRAPHIQUES DANS L'ASTHME BRONCHIQUE
Authors: R. PANZANI
Journal: Allergy (1959)

SUMMARY Two hundred and forty EEG observations bring out this conclusion: in asthma 65 per cent of the observed cases show a disturbed cortical electro genesis leading to the hypothesis of a hyperexcitability or less frequently a hypoexcitability and possibly an actual depression of meso‐diencephalic reticular substance. The first hypothesis was that this major or minor dysfunction of the reticular substance is one of the causes of asthma and not the consequence of an oedema of the brain caused by repeated attacks. Out recent EEG studies would incline in some cases in favour of the latter hypothesis. We have observed in 17 out of 80 regularly followed cases, that minor anomalies in adults and even major anomalies in children have disappeared. Asthma has been practically always allergic in children so we think that the electrical anomalies which have spontaneously disappeared were due to an allergic oedema of the brain.
Lung Cancer: Natural History, Prognosis, and Therapy
Authors: Robert M. Senior
Journal: JAMA (1977)

The five-year survival of patients with lung cancer has remained at a dismal 5% to 10% for decades. Looking at this statistic alone, however, conceals important advances in our knowledge about lung cancer. This book focuses on some of the advances. Of 18 chapters six concern epidemiology, tumor growth, histology classifcation, prognosis in relation to stage of disease, and death from nonmalignant causes. The remaining chapters cover therapy, especially chemotherapy, radiation treatment, immunotherapy, and supportive care. Separate chapters deal with the all-too-common problems of caring for patients whose lung cancer is either locally unresectable or metastatic. The editors have written approximately half of the material, with ten contributors providing the rest. The book contains information that will be useful to anyone confronted with responsibility for patients with lung cancer. Some is directly applicable to patient care, but much is rather conceptual and thus would be of particular interest to oncologists
Programmable Open-Loop Micropump Insulin Delivery System
Authors: A H Clemens
Journal: Diabetes Care (1980)

The application of "closed-loop" glucose-controlled insulin infusion in metabolic research and in the management of diabetic patients in acute emergency situations has stimulated the development of portable insulin infusion devices intended for the long-term stabilization of glucose levels. Since in vivo glucose sensors with long-term accuracy and reliability are not yet available, the currently projected portable insulin infusion systems are the "open-loop" type. Such systems should be reliable, safe, programmable, lightweight, and small in size. In addition, the power requirements should be as low as possible to avoid the need for heavy batteries and/or periodic recharging. In turn, these requirements govern the design criteria for the most essential component of such an instrument, the insulin delivery pump. A micropump has been developed in an attempt to meet all desirable design features. Evaluation data are presented.
Dedifferentiation of vascular smooth muscle cells involved in remodeling of small intramyocardial coronary arteries distal to a flow-limiting epicardial coronary artery stenosis
Authors: Huashan Hong, Sergei Aksenov, Xiaoming Guan, Joseph Marrak, David Waters, John T. Fallon, Chunguang Chen
Journal: Journal of the American College of Cardiology (2002)
An A Priori Study Of Nitric Oxide Synthase Type 2 (nos2) Promoter Polymorphisms And Exhaled Nitric Oxide Levels In African Americans
Authors: M LEVESQUE, A ALLEN, D HAUSWIRTH, S MERVINBLAKE, C FERNANDEZ, K PATCH, K ALEXANDER, S ALLGOOD, P MCNAIR, J SUNDY
Journal: Journal of Allergy and Clinical Immunology (2008)
The Childhood Asthma Control Test∗: Retrospective determination and clinical validation of a cut point to identify children with very poorly controlled asthma
Authors: Andrew H. Liu, Robert S. Zeiger, Christine A. Sorkness, Nancy K. Ostrom, Bradley E. Chipps, Kathleen Rosa, Maria E. Watson, Michael S. Kaplan, John R. Meurer, Todd A. Mahr, Michael S. Blaiss, Elisabeth Piault-Louis, Jeffrey McDonald
Journal: Journal of Allergy and Clinical Immunology (2010)
Clinical Lecture on a Case of Influenza: With Persistent Headache and Jarring Sensation; Failure of Tonics and Stimulants; Success of Bromide of Potassium and a Sternutatory
Authors: C. H. Jones
Journal: BMJ (1870)

He had been ill nine weeks.He had at first a violent cold in the head, anid much running from the nose, but not much cough.He had still some remains of this cold.He had had a great deal of retching; no actual vomiting.He complained of pain in the head from the tem- ples to the occiput; most pain in the left temple.He had a ringing noise in hiis left ear, and felt always as if there were " a jumping in the head which jarredhim all over".The head was not tender now to tapping; some days it was very much so.He had had, and still had, a craving appetite; lhe wanted something to eat every two or three hours, and, if be did not get it, felt as if he would sink through the bed, and experienced a dull weary pain at the upper abdomen.He felt very low, and sweated profusely at night.He had much flatu- lence.Tongue moist.He had no pain after eating.The bowels were open daily.Pulse 8I, of fair apparent force; temperature 37.3 deg.(99.I deg.)The urine was pale, not albuminous, of specific gravityI012.He had no paralysis.He denied having had syphilis and gonorrhcea.He had been treated by a medical man, who told him he had a water-congestion on the brain, blistered his neck and occiput, and put a leech behind each ear, which bled freely.He was put on ordinary diet, with four ounces of port wine; and was ordered to take the following draught three times a day.Ammonioe carbonatis gr.iv; spirit.ether.sulph.comp.3ss; tinc- turoe cinchonie 3ss; decocti cinchonme 5j.M. Jan. 3rd.He had less sweating.His head was not in any pain as lonig as he kept it warm; but the ringing and throbbing continued.The forehead was cool.He did not sleep soundly.He still felt very low.Puilse good and steady, 72
Ultrasonic nebulization of hypertonic solution: a new method for obtaining specimens from nasal mucosa for morphologic and biochemical analysis in allergic rhinitis
Authors: G. Melillo, G. Balzano, F. Stefanelli, C. Iorio, E. De Angelis, E. Melillo
Journal: Allergy (1998)

Various techniques are used to collect specimens from the nasal mucosa for morphologic and biochemical analysis. The purpose of this study was to devise a method that overcomes some of the disadvantages (e.g., invasive procedure, samples not suitable for cytologic and biochemical analysis, lack of standardization, and poor reproducibility) of these techniques. The new method requires subjects, with neck extended, to inhale an ultrasonic nebulization of a hypertonic (3% NaCl) solution (UNHS) for 5 min. They then blow their nose into a Petri dish, one nostril at a time with the other one blocked. The secretions are dispersed with 0.1% dithiothreitol in phosphate buffer solution for 20 min. Total cell count (TCC) is evaluated, and the cellular suspension is divided into two aliquots: one is centrifuged and the supernatants are collected for eosinophil cationic protein (ECP) measurements; the other is cytocentrifuged and the slides, stained with Diff‐Quik, are used for differential cell count. The results obtained with the UNHS and nasal lavage (NL) methods were compared. Eleven nonatopic healthy subjects and 19 allergic rhinitic patients were studied. Total cell count (×10 5 ) was significantly higher with UNHS than with NL (13.0±12.3 vs 1.911.6; P&lt;0.0]) The differential cell count was similar with the two procedures. ECP levels (μg/l) were higher with UNHS than with NL (39.1+38.2 V.S 16.7±41.2; P&lt;0.01). For evaluation of reproducibility, four healthy and six rhinitic subjects underwent UNHS on two occasions within 5 days, and the results of two samples (sample 1 vs sample 2) were analyzed. Reproducibility was good as to TCC, differential cell count, and ECP
Thrombocytopenia in Alcoholics
Authors: JOHN LINDENBAUM
Journal: Annals of Internal Medicine (1968)

Ten episodes of thrombocytopenia in five chronic alcoholics with delirium tremens are described. Platelet counts rapidly returned to normal after withdrawal of alcohol with a subsequent thrombocytosis. None of the patients had cirrhosis, splenomegaly, or evidence of folic acid deficiency. Thrombocytopenia recurred in three patients 4 to 8 weeks after discharge from hospital and resumption of ethanol intake. It is postulated that the thrombocytopenia was a direct effect of alcohol intoxication by a mechanism as yet unknown.
Statutory brain death?
Authors: D. H. Mills
Journal: JAMA (1974)

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