tbuckley/cabot-search huggingface dataset:
Abstract 18238: Markedly Elevated Pericardial Fluid Levels of N-terminal Probrain Natriuretic Peptide are Involved in Left Ventricular Systolic Dysfunction with Aortic Stenosis and Ischemic Heart Disease
Authors: Shoichi Miyamoto, Toshiaki Izumi, Eisaku Nakane, Takao Katoh, Moritoshi Funasako, Masahiro Kimura, Kanae Su, Mitsumasa Okano, Yuki Kimura, Takayuki Sekihara, Hideyuki Hayashi, Jun Iida, Manabu Morishima, Koji Ueyama, Tetsuya Haruna, Masatoshi Fujita, Moriaki Inoko
Journal: Circulation (2014)

Background: Increasing evidence suggests that N-terminal probrain natriuretic peptide (NT-proBNP) may be a more discerning marker for the detection and evluation of heart failure than BNP. However, fundamental mechanisms that contribute to the heart failure in aortic stenosis (AS) and ischemic heart disease (IHD) via NT-proBNP remain unclear. Objectives: The purpose of this study was to investigate whether NT-proBNP represents autocrine/paracrine factors and are accumulated in pericardial fluid (PF) in patients with AS and IHD. Methods: With an electrochemiluminescent immunoassay, we measured the concentrations of NT-proBNP in serum and pericardial fluid simultaneously in 48 patients undergoing aortic valve replacement and 101 patients undergoing coronary artery bypass grafting. We investigated the correlation of NT-proBNP levels in serum or PF with hemodynamic variables. Results: In patients with AS, the PF levels of NT-proBNP were markedly elevated compared with serum levels (10747±17619 pg/ml vs. 2828±7787 pg/ml, p=0.0054). In patients with IHD, the PF levels of NT-proBNP were markedly elevated compared with serum levels (7224±16345 pg/ml vs. 764±1199 pg/ml, p=0.0001). In AS, NT-proBNP levels in PF but not in serum had closer relations with left ventricular (LV) end-diastolic (r=0.338, p=0.0463) and systolic volume indexes (r=0.551, p=0.0005). In AS, NT-proBNP levels in PF but not in serum inversely correlated with LV ejection fraction (EF) (r=-0.510, p=0.0002). In IHD, NT-proBNP levels in PF but not in serum had closer relations with left ventricular (LV) end-diastolic (r=0.493, p&lt;0.0001) and systolic volume indexes (r=0.582, p&lt;0.0001). NT-proBNP levels in PF but not in serum inversely correlated with LV ejection fraction (EF) (r=-0.406, p&lt;0.0001). NT-proBNP levels in serum and PF is higher in AS due to pressure overload than in IHD due to volume overload. Conclusion: Increased PF concentrations of NT-proBNP reflects the direct actions as a local mediator against cardiac pressure overload in AS patients and cardiac voume overload in IHD patients with left ventricular systolic dysfunction.
ANALYSIS OF CLINICAL SPECIMENS BY HYBRIDISATION WITH PROBE CONTAINING REPETITIVE DNA FROM PLASMODIUM FALCIPARUM
Authors: Lena Franzén, Raad Shabo, Hedvig Perlmann, Hans Wigzell, Gunnar Westin, Lena Aslund, Torgny Persson, Ulf Pettersson
Journal: The Lancet (1984)
Recombinant Thrombomodulin of Different Domains for Pharmaceutical, Biomedical, and Cell Transplantation Applications
Authors: Lin Wang, Rui Jiang, Xue‐Long Sun
Journal: Medicinal Research Reviews (2013)

Abstract Thrombomodulin (TM) is a membrane glycoprotein mainly expressed by vascular endothelial cells and is involved in many physiological and pathological processes, such as coagulation, inflammation, cancer development, and embryogenesis. Human TM consists of 557 amino acids divided into five distinct domains: N‐terminal lectin‐like domain (designated as TMD1); six epidermal growth factor (EGF)‐like domain (TMD2); Ser/Thr‐rich domain (TMD3); transmembrane domain (TMD4); and cytoplasmic tail domain (TMD5). The different domains are responsible for different biological functions of TM. In the past decades, various domains of TM have been cloned and expressed for TM structural and functional study. Further, recombinant TMs of different domains show promising antithrombotic and anti‐inflammatory activity in both rodents and primates and a recombinant soluble TM has been approved for therapeutic application. This review highlights recombinant TMs of diverse structures and their biological functions, as well as the complex interactions of TM with factors involved in the related biological processes. Particularly, recent advances in exploring recombinant TM of different domains for pharmaceutical, biomedical, and cell transplantation applications are summarized.
FRANK HEMATURIA AS A MANIFESTATION OF INFECTIOUS MONONUCLEOSIS
Authors: W T THOMPSON, C PITT
Journal: Annals of Internal Medicine (1950)

Case Reports1 November 1950FRANK HEMATURIA AS A MANIFESTATION OF INFECTIOUS MONONUCLEOSISW. T. THOMPSON JR., M.D., CULLEN PITT, M.D.W. T. THOMPSON JR., M.D.Search for more papers by this author, CULLEN PITT, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-33-5-1274 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThat the urinary tract can be involved during the course of infectious mononucleosis is an established fact. However, this is not a frequent or generally recognized complication. Tidy and Morley1reported an incidence of 6 per cent in 270 cases analyzed in 1921, and Wechsler2found abnormal urinary manifestations in 3 per cent of 556 cases observed during an army epidemic. This 3 per cent included those cases showing red and white blood cells, albumin, and hyaline and granular casts. He referred to two cases of frank hematuria. Many series of cases reported in the literature over a period of...Bibliography1. TidyMorley HLEB: Glandular fever, Brit. M. J. 1: 452 (March 26) 1921. CrossrefMedlineGoogle Scholar2. WechslerRosenblumSills HFAHCT: Infectious mononucleosis: report of epidemic in army post, Ann. Int. Med: 25: 113 (July) 1946; Part II, Ann. Int. Med. 25: 236 (August) 1946. LinkGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Richmond, Virginia*Received for publication September 18, 1948.From the Medical Service of St. Luke's Hospital, Richmond, Va. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byGross hematuria as a manifestation of infectious mononucleosisAcute renal failure and infectious mononucleosisKlinik und Therapie der GlomerulonephritidenTreatment of Infectious Mononucleosis; a Review of 1500 Cases 1 November 1950Volume 33, Issue 5Page: 1274-1275KeywordsAlbuminsMedical servicesUrinary tract infectionsWhite blood cells ePublished: 1 December 2008 Issue Published: 1 November 1950 PDF downloadLoading ...
Pulsed Dye Laser Treatment of Acne Vulgaris
Authors: A C Chu
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Economics Notes: Economic modelling before clinical trials
Authors: D. J Torgerson
Journal: BMJ (2002)

Clinical trials can be expensive and technically difficult to execute. Ideally, before a trial is undertaken three questions require answering. Firstly, what is the amount of clinical uncertainty within a given area. Secondly, what is the likely achievable benefit from a given trial relative to some other alternative use of the research resources. Thirdly, given that a trial is indicated, what should its size and design be?

The first question is usually resolved through the use of a prior systematic review of the literature. Indeed, major funding agencies such as the Medical Research Council (MRC) ask whether a systematic review has been undertaken when funding decisions are made. The second issue regarding the likely benefit relative to cost is not usually considered.1 However, the inclusion of some economic modelling during the early design stages of a trial can inform not only the scale of the potential benefit of the proposed research relative …
The impact of the 2003 heat wave on daily mortality in England and Wales and the use of rapid weekly mortality estimates
Authors: H Johnson, S Kovats, G McGregor, J Stedman, M Gibbs, H Walton
Journal: Eurosurveillance (2005)

This paper describes a retrospective analysis of the impact of the 2003 heat wave on mortality in England and Wales, and compares this with rapid estimates based on the Office for National Statistics routine weekly deaths reporting system. Daily mortality data for 4 to 13 August 2003, when temperatures were much hotter than normally seen in England, were compared with averages for the same period in years 1998 to 2002. The August 2003 heat wave was associated with a large short-term increase in mortality, particularly in London. Ozone and particulate matter concentrations were also elevated during the heat wave. Overall, there were 2139 (16%) excess deaths in England and Wales. Worst affected were people over the age of 75 years. The impact was greatest in the London region where deaths in those over the age of 75 increased by 59%. Estimated excess mortality was greater than for other recent heat waves in the United Kingdom. The estimated number of deaths registered each week is reported by the Office for National Statistics. The first clear indication of a substantial increase in deaths was published on 21 August 2003. This provided a quick first estimate of the number of deaths attributable to the heat wave and reflected the pattern of daily deaths in relation to the hottest days, but underestimated the excess when compared with the later analysis.
Abstract 727: Does gene transfer of gulono-lactone oxidase into human hepatocellular carcinoma cells restore ascorbate biosynthesis
Authors: Teresa Flett, Elizabeth Campbell, Elisabeth Phillips, Margreet CM Vissers, Gabi U. Dachs
Journal: Cancer Research (2014)

Abstract Humans are unable to synthesise ascorbate (vitamin C) due to a mutation in the gene for gulono-lactone oxidase (Gulo), the terminal enzyme in the synthesis pathway. Ascorbate is a vital micronutrient required for many biological functions, including its action as a vital cofactor for Cu- and Fe-containing enzymes. As enzyme cofactor for HIF-hydroxylases, it is important for the regulation of hypoxia-inducible factor-1 (HIF-1), which governs cancer growth and spread. A lack of ascorbate increases both the level and activity of HIF-1 in cell culture. This study aimed to restore ascorbate synthesis to human cells and to determine the effect of intracellular ascorbate biosynthesis on HIF-1 levels. HepG2 cells were gene modified using Lipofectamine LTX with a plasmid encoding the mouse Gulo cDNA and a control plasmid encoding the green fluorescent protein (GFP). Modified HepG2 cells were tested for genomic incorporation by PCR, for Gulo enzyme production by Western blotting, and ascorbate synthesis by high performance liquid chromatography with electrochemical detection (HPLC-ECD). Protein levels of HIF-1alpha in hypoxic cells were measured by Western blot analysis. Parental HepG2 cells accumulated a mean of 120 nmol ascorbate per 1 million cells when loaded for 24h with 0.5 mM ascorbate. Gene-modified HepG2Gulo cells demonstrated a change in morphology and an increase in adherence, compared to HepG2GFP and parental HepG2 cells. A PCR-positive stable clone was able to synthesise ascorbate but only when the Gulo substrate, L-gulono-1,4-lactone, was supplied. Intracellular ascorbate levels then reached 5% of saturation levels. Hypoxic HIF-1 accumulation was reduced by addition of gulonolactone to this clone. However, gulonolactone also reduced HIF-1 levels in parental HepG2 cells, suggesting that it may similarly act as cofactor for the HIF-hydroxlases. This study indicated that the ascorbate biosynthesis pathway in human cells is significantly modified and may contain numerous non-functional members besides gulono-lactone oxidase. Future biochemical studies will determine which other enzymes are non-functional. Citation Format: Teresa Flett, Elizabeth Campbell, Elisabeth Phillips, Margreet CM Vissers, Gabi U. Dachs. Does gene transfer of gulono-lactone oxidase into human hepatocellular carcinoma cells restore ascorbate biosynthesis. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 727. doi:10.1158/1538-7445.AM2014-727
Targeting the vitamin D receptor inhibits the B cell‐dependent allergic immune response
Authors: B. Hartmann, G. Heine, M. Babina, A. Steinmeyer, U. Zügel, A. Radbruch, M. Worm
Journal: Allergy (2010)

To cite this article: Hartmann B, Heine G, Babina M, Steinmeyer A, Zügel U, Radbruch A, Worm M. Targeting the vitamin D receptor inhibits the B cell‐dependent allergic immune response. Allergy 2011; 66 : 540–548. Abstract Background: 1α,25‐dihydroxyvitamin D 3 (calcitriol), the biologically active form of vitamin D, is an immunomodulatory hormone, e.g. it inhibits IgE synthesis in B cells. As its clinical application is limited by hypercalcemia, synthetic vitamin D receptor (VDR) agonists that mediate immunomodulatory activities without adverse hypercalcemic effects are of great interest. This study aimed to investigate the impact of a low‐calcemic VDR agonist on the IgE immune response in vitro and in vivo . Methods: Human peripheral B cells were cultured under IgE inducing conditions in the presence of VDR ligands. B cells were analyzed by quantitative RT‐PCR, enzyme‐linked immunosorbent assays, enzyme‐linked immunospot technique, and flow cytometry. BALB/c mice were sensitized with ovalbumin (OVA)/alum followed by the therapeutic VDR agonist treatment and analyzed regarding the humoral immunoglobulin profile. Results: The natural VDR ligand calcitriol, but also a low‐calcemic VDR agonist, profoundly suppressed IgE production by human peripheral B cells by 63.9 ± 5.9%. The potential mechanisms involved are the reduction of the transcript for activation‐induced deaminase (AID) and the reduction of IgE immunoglobulin‐secreting cells by 68.1 ± 12.7%. By using an in vivo approach, we finally demonstrate that the humoral IgE response in a type I allergy mouse model was impaired by the VDR agonist. Conclusion: Our results show that targeting the VDR modulates the humoral immune response including IgE. Whether it might be useful for clinical applications has to be determined in appropriate clinical trials.
Driving after hernia surgery
Authors: J F Colin
Journal: BMJ (2001)

EDITOR—Amid in his editorial and Ismail et al in their short report say that surgeons traditionally advise patients recovering from groin hernias not to drive for a month or two and recommend national guidelines be developed. 1 2 In 1976 we published the effects of surgical operation on the “brake clutch simulator”3 and showed that patients who had an inguinal hernia repair under general anaesthetic were able to perform an emergency stop in exactly the same time as they could preoperatively eight days after operation.

In an average car braking system, in 1975, a pedal force of 600 lb per square inch would produce an emergency stop of 0.87 g deceleration from 30 mph. The brake clutch simulator consisted of an adjustable car seat with pedals attached via hydraulic links and cylinders to load syringes, together with gauges to monitor the pressure in the brake and clutch lines, and a transducer for pressure recording. Microswitches were provided …
396. Factors Affecting Retroviral Gene Transfer to Human CD34+ Cells
Authors: Youngtae Hong, Seungshin Yu, Sujeong Kim, Joonggon Kim, Sunyoung Kim
Journal: Molecular Therapy (2004)

Retrovirus-mediated gene transfer is a useful technology in studying the biology of hematopoietic stem cells (HSCs) as well as in developing gene therapy products for a variety of human diseases. One of the most important factors determining the success of these studies is the number of HSCs receiving the gene of interest. We tested various parameters for their influences on gene transfer efficiency to CD34+ cells derived from bone marrow. Based on literature survey, three medium formulations of CD34+ cells have been compared for their effects on gene delivery efficiency and differentiation of them. We also tested whether FBS that used in medium formulation, could be replaced with human serum or synthetic material. Formulation A, consisting of stem cell factor, Flt-3 ligand, thrombopoietin, and IL-3, provided optimum results in that it maintained the highest percentage of CD34+ cells during the culture as well as produced the highest gene delivery efficiency. It was found that the synthetic serum substitute containing bovine serum albumin, insulin and human transferrin could replace the fetal bovine serum present in original formulation A without compromising gene transfer efficiency. When the transduction procedure was repeated three times, the gene could be delivered in up to 60% of the cell population. Gene delivery efficiency was comparable between CD34+ cells derived from bone marrow and mobilized peripheral blood. Our data could be useful in designing the procedure for stem cell gene therapy and providing a basis for further improving the conditions for gene transfer to various HSCs.
Abstract 9946: Impact of Sex Differences on Invasive Measures of Coronary Microvascular Dysfunction in Patients With Angina in the Absence of Obstructive Coronary Artery Disease
Authors: Yuhei Kobayashi, Yasuhiro Honda, William F Fearon, Shigemitsu Tanaka, Peter J Fitzgerald, David P Lee, Alan C Yeung, Jennifer A Tremmel
Journal: Circulation (2014)

Background: Coronary microvascular dysfunction is associated with worse long-term outcomes, especially in women. Coronary flow reserve (CFR) is typically used to interrogate microvascular function; however its variability limits reliability. Alternatively, the index of microcirculatory resistance (IMR) is a direct measure of the microvasculature, but has been less thoroughly studied. We investigated sex differences in CFR and IMR in patients with angina in the absence of obstructive coronary artery disease (CAD). Methods: We prospectively enrolled 117 women and 40 men with angina in the absence of obstructive CAD. We performed CFR, IMR, fractional flow reserve (FFR), and quantitative coronary angiography (QCA) in the left anterior descending artery. Coronary flow was assessed with a thermodilution method by obtaining mean transit time (Tmn: an inverse correlate to absolute flow) at rest and hyperemia. IMR was measured as distal coronary pressure at hyperemia x hyperemic Tmn. Results: All patients had minimal or no atherosclerosis by QCA (%diameter stenosis: 23.2±12.3%), and epicardial disease was milder in women (FFR: 0.88±0.04 vs. 0.87±0.04, p=0.04). IMR was similar between the sexes (20.7±9.8 vs. 19.1±8.0, p=0.45), but CFR was lower in women (3.8±1.6 vs. 4.8±1.9, p=0.004). This was primarily due to a shorter resting Tmn in women (p=0.005), while hyperemic Tmn was identical (p=0.79) (Figure). The shorter resting Tmn in women, reflecting increased resting coronary flow, accounted for the lower CFR. In multivariate analysis, female sex was an independent predictor of lower CFR and shorter resting Tmn, but not a predictor of IMR or hyperemic Tmn. Conclusions: Despite women and men having similar microvascular function by IMR, CFR is lower in women. This discrepancy appears to be due to differences in resting coronary flow between the sexes. The impact of sex differences should be considered in interpretation of physiologic indices using resting coronary flow.
ECOLOGY OF SHIPS OF INNER SPACE
Authors: Harry J. Alvis
Journal: Annals of Internal Medicine (1959)

Article1 December 1959ECOLOGY OF SHIPS OF INNER SPACEHARRY J. ALVISHARRY J. ALVISAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-51-6-1329 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe ecology of sealed chambers can be divided into three levels of self-sufficiency. There is the simplest level, that of sealing the subject or subjects in with some oxygen, some carbon dioxide absorbent, some food and water, and leaving them there as long as they can manage to survive. The second level may be described as partially regenerative. The food supply is finite, but the processes of providing oxygen, removing carbon dioxide, and providing water are regenerative and can continue indefinitely. In this system, waste is thrown away.The third and highest level is that of the closed ecologic system....Bibliography1. Rigsbee JT: Man made air, U. S. Nav. Inst. Proc. 85: 30 (Jan.) 1959. Google Scholar2. Ebersole JH: Submarine medicine on Nautilus and Seawolf , U. S. Armed Forces M. J. 9: 851 (June) 1958. MedlineGoogle Scholar3. Alvis HJ: Submarine medicine—an occupational specialty, New England J. Med. 256: 21-25 (Jan. 3) 1957. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: HARRY J. ALVIS*Received for publication May 20, 1959.Presented at the Fortieth Annual Session of The American College of Physicians, Chicago, Illinois, April 23, 1959.The opinions or assertions contained herein are the private ones of the writer, and are not to be construed as official or reflecting the views of the Navy Department or the Naval Service at large.Requests for reprints should be addressed to Captain Harry J. Alvis (MC), Submarine Medical Division, Bureau of Medicine and Surgery, U. S. Navy, Washington, D. C. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 December 1959Volume 51, Issue 6Page: 1329-1334KeywordsCarbon dioxideEcologyFoodOxygen ePublished: 1 December 2008 Issue Published: 1 December 1959 PDF downloadLoading ...
Outpatient Poem #2
Authors: Michael Diebert
Journal: JAMA (2003)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal