tbuckley/cabot-search huggingface dataset:
Abstract 4306: Bioconjugation of near infrared dyes by PEGylation improves pharmacokinetics and allows for better labeling and imaging of human gastrointestinal cancers
Authors: Ali A. Maawy, Yukihiko Hiroshima, George A. Luiken, Yong Zhang, Robert M. Hoffman, Michael Bouvet
Journal: Cancer Research (2014)

Abstract NIR dyes are becoming increasingly popular due to their good tissue penetration, low tissue autofluorescence and resistance to hemoglobin quenching. However, they are still limited by lower hydrophilicity and poor chemical and photo-stability in-vivo. We aimed to try to improve these properties by covalent modification of these dyes by addition of polyethylene glycol (PEG) chains. We studied 2 commercially-available NIR dyes, DyLight 650 and 750, to evaluate how PEGylation affects the pharmacokinetics, biodistribution and imaging. PEGylated and non-PEGylated DyLight 650 and 750 dyes were conjugated to a chimeric anti-CEA antibody and were injected intravenously into nude mice. Serum samples were collected at various time points to determine serum concentrations and clearance kinetics. Biodistribution was determined by tissue sonication and analyzing tissue concentration profiles over time. PEGylated dyes had significantly higher serum concentrations than non-PEGylated dyes with p=0.005 for the 650 dyes and p&amp;lt;0.001 for the 750 dyes. PEGylated dyes had significantly lower liver concentrations (p=0.03 for the 650 dyes; p=0.002 for the 750 dyes) and higher renal concentrations (p=0.003 for the 650 dyes and p&amp;lt;0.001 for the 750 dyes) compared to non-PEGylated dyes. Human pancreatic tumors subcutaneously implanted into nude mice were labeled with antibody-dye conjugates and serially imaged. Labeling with PEGylated dyes resulted in significantly brighter tumors compared to the non-PEGylated dyes (p&amp;lt;0.001 for the 650 dyes; p=0.01 for 750 dyes). In a liver metastasis model of colon cancer, PEGylated dyes showed bright labeling of metastatic tissue with good contrast between normal and malignant tissue. PEGylation of the NIR dyes DyLight 650 and 750 significantly changes their pharmacokinetics and biodistribution, allowing higher serum concentrations, longer half-life, brighter tissue labeling and decreased accumulation in lymphoid organs. This allows for high fidelity imaging of metastatic disease to any lymphoid or hemoglobin dense organ such as the liver. Citation Format: Ali A. Maawy, Yukihiko Hiroshima, George A. Luiken, Yong Zhang, Robert M. Hoffman, Michael Bouvet. Bioconjugation of near infrared dyes by PEGylation improves pharmacokinetics and allows for better labeling and imaging of human gastrointestinal cancers. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4306. doi:10.1158/1538-7445.AM2014-4306
Epidemiology and aetiology of basal cell carcinoma
Authors: J. Roewert-Huber, B. Lange-Asschenfeldt, E. Stockfleth, H. Kerl
Journal: British Journal of Dermatology (2007)

Journal Article Epidemiology and aetiology of basal cell carcinoma Get access J. Roewert‐Huber, J. Roewert‐Huber Department of Dermatology Venereology and Allergy, Skin Cancer Center Charité, University Hospital of Berlin, Berlin, Germany Correspondence: Joachim Roewert‐Huber. E‐mail: joachim.roewert@charite.de Search for other works by this author on: Oxford Academic Google Scholar B. Lange‐Asschenfeldt, B. Lange‐Asschenfeldt Department of Dermatology Venereology and Allergy, Skin Cancer Center Charité, University Hospital of Berlin, Berlin, Germany Search for other works by this author on: Oxford Academic Google Scholar E. Stockfleth, E. Stockfleth Department of Dermatology Venereology and Allergy, Skin Cancer Center Charité, University Hospital of Berlin, Berlin, Germany Search for other works by this author on: Oxford Academic Google Scholar H. Kerl H. Kerl Department of Dermatology, University of Graz, Graz, Austria Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 157, Issue s2, 1 December 2007, Pages 47–51, https://doi.org/10.1111/j.1365-2133.2007.08273.x Published: 01 December 2007
Abstract 154: Continuous Intravenous Administration of Adrenaline May Prevent the Biphasic Reaction of Anaphylaxis
Authors: Kenji Fujizuka, Minoru Nakano, Mitsunobu Nakamura, Takayuki Ogura
Journal: Circulation (2014)

Objective: Anaphylaxis is a multi-system syndrome resulting from the release of chemical mediators upon exposure to an allergen. Biphasic anaphylactic reactions may occur due to secondary elevation of the chemical mediators following the initial reaction. The initial treatment of anaphylaxis generally includes adrenaline injection, which results in decreased chemical mediator release. We hypothesize that continuous intravenous administration, rather than intramuscular injection of adrenaline, may inhibit the release of chemical mediators following the initial reaction, and thereby prevent biphasic reactions. Accordingly, in this study, we aimed to investigate the occurrence of biphasic reactions according to the type of administration of adrenaline. Methods: We included patients with anaphylaxis admitted to our hospital, who were treated with adrenaline between April 2005 and March 2014. We retrospectively investigated the occurrences of biphasic reactions after admission in patients treated with continuous intravenous adrenaline or intramuscular adrenaline injections. Patients were excluded if they had received intravenous bolus or complex methods of administration. The risk of biphasic reactions according to the method of administration was analyzed by logistic regression. Results: A total of 104 patients were included in the study (continuous intravenous administration: 56, intramuscular injection: 48). Eight patients (7.69%) experienced biphasic reactions, out of which one had received continuous intravenous administration, and seven had received intramuscular injection (p = 0.02). No patient had received adrenaline during the biphasic reaction in the continuous intravenous administration group compared to the three patients in the intramuscular injection group (p = 0.10). Logistic regression analysis revealed that the odds ratio of biphasic reactions for continuous intravenous administration vs. intramuscular injection of adrenaline was 0.11 (p = 0.04, 95% confidence interval: 0.01-0.90). Conclusions: Continuous intravenous administration of adrenaline may prevent the biphasic reaction of anaphylaxis.
Effect of specific siRNA targeting uPAR and MMP-9 in medulloblastoma
Authors: Chandramu Chetty, Jasti S. Rao, Subramanyam Chittivelu, Pushpa Joseph, Sajani Lakka
Journal: Cancer Research (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

4363 

The molecular mechanisms underlying the spread of cancer are complex and have only recently begun to be elucidated. In particular, the cellular mechanisms leading to metastatic disease in medulloblastoma (MB), the most common malignant brain tumor in childhood, are mainly unknown. The initial stage of intraspinal spread is characterized by both seeding of tumor cells on the arachnoid or pia mater and the lysis of collagenous barriers to enhance angiogenesis and local invasion, especially extracellular matrix (ECM) degradation. The cooperative expression of a family of adhesion receptors called integrins and cell surface proteinases ( e.g. , uPA, uPAR and MMPs) mediates these angiogenic and invasive processes. A significant association of urokinase plasminogen activator (uPA) and matrix metalloprotease-9 (MMP-9) expression with survival and M-stage indicates that these proteases may modulate the survival of medulloblastoma patients. RNA interference (RNAi) is a conserved mechanism in which double-stranded, small, interfering RNAs (siRNAs) trigger a sequence-specific, gene-silencing process. In this study, we describe the inhibition of urokinase plasminogen activator receptor (uPAR) and MMP-9 using siRNA expression vectors targeted to sequences specific to the coding regions of uPAR (pU) and MMP-9 (pM). We also compare these effects to an expression vector carrying siRNA against both these proteases (pUM). Here, we have targeted uPAR and MMP-9 singly and in combination in vitro to evaluate the anti-invasive and anti-proliferative effects of uPAR and MMP-9 downregulation. We also evaluated these constructs for in vivo anti-tumor activity. MMP-9 and uPAR expression was determined using zymography, western blot, and semiquantitative polymerase chain reaction. Cellular invasion was evaluated using matrigel and spheroid invasion assays. Cell proliferation was determined by MTT assay. Our results demonstrate that the siRNA constructs for uPAR and MMP-9 are capable of specific and functional silencing of gene expression and invasion in vitro in medulloblastoma cells. Moreover, treatment of DAOY meduloblastoma cells implanted orthotopically in mice with the siRNA constructs resulted in significant decreases in tumor size, which translated to increases in survival time. Further analysis of tumors showed that the reduced sizes correlated with a decrease in mean vessel density and an increase in tumor cell apoptosis. Statistical analysis indicates that targeting both uPAR and MMP-9 genes with a single vector demonstrated a clear synergistic effect as compared to targeting either uPAR or MMP-9 alone.
Nanoparticles and Mucus
Authors: Tracy Hampton
Journal: JAMA (2007)

New research has revealed that large nanoparticles can penetrate mucus barriers and may therefore provide an efficient means of mucosal drug delivery (Lai SK et al. Proc Natl Acad Sci U S A. doi:10.1073/pnas.0608611104 [published online January 23, 2007]).
Mast cell adhesion to bronchial smooth muscle in asthma specifically depends on CD51 and CD44 variant 6
Authors: P.‐O. Girodet, A. Ozier, T. Trian, H. Begueret, O. Ousova, J.‐M. Vernejoux, P. Chanez, R. Marthan, P. Berger, J. M. Tunon de Lara
Journal: Allergy (2010)

Mast cells infiltrate the bronchial smooth muscle (BSM) in asthmatic patients, but the mechanism of mast cell adhesion is still unknown. The adhesion molecules CD44 (i.e. hyaluronate receptor) and CD51 (i.e. vitronectin receptor) are widely expressed and bind to many extracellular matrix (ECM) proteins. The aims of the study are (i) to identify the role of ECM in mast cell adhesion to BSM and (ii) to examine the role of CD51 and CD44 in this adhesion.Human lung mast cells, human mast cell line (HMC-1), and BSM cells from control donors or asthmatic patients were cultured in the presence/absence of various cytokines. Mast cell-BSM interaction was assessed using (3)H-thymidine-pulsed mast cells, confocal immunofluorescence, or electron microscopy. Adhesion molecules expression and collagen production on both cell types were evaluated by quantitative RT-PCR, western blot, and flow cytometry.Mast cell adhesion to BSM cells mostly involved type I collagen of the ECM. Such an adhesion was increased in normal BSM cells under inflammatory condition, whereas it was maximal in asthmatic BSM cells. Blockade of either CD51 or CD44 significantly decreased mast cell adhesion to BSM. At the molecular level, protein and the transcriptional expression of type I collagen, CD51 or CD44 remained unchanged in asthmatic BSM cells or in mast cells/BSM cells under inflammatory conditions, whereas that of CD44 variant isoform 6 (v6) was increased.Mast cell-BSM cell adhesion involved collagen, CD44, and CD51, particularly under inflammatory conditions. CD44v6 expression is increased in asthmatic BSM cells.
TRANSVERSE MYELITIS FOLLOWING MUMPS
Authors: A. C. Fortney
Journal: Annals of Internal Medicine (1937)

Case Reports1 October 1937TRANSVERSE MYELITIS FOLLOWING MUMPSA. C. FORTNEY, M.D.A. C. FORTNEY, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-11-4-682 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptAlthough it is not unusual to find meningitis, encephalitis, neuritis, and delirium as complications of mumps, myelitis is most uncommon. Woltman and McKaig reported such a case in 1933, and at that time a very close search of the literature revealed only one other case, that of Warrington's reported in 1914. However, a third case was reported by Kuligowski in October 1933. I should like to add another to those reported.CASE REPORTMr. N. E., white male, aged 39, hardware dealer, first contracted mumps on December 26, 1935. Involvement first appeared in the right parotid, and four days later...Bibliography WOLTMANMCKAIG HWCB: Neurologic complications of epidemic parotitis, Arch. Neurol. and Psychiat., 1934, xxxi, 794-808. Google Scholar WARRINGTON WB: Acute generalized infective paralysis in adults, Clin. Jr., 1914, xliii, 296. Google Scholar KULIGOWSKI ZW: Un cas de myelite curlinne, Revue Neurol., 1933, xi. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Fargo, North Dakota*Received for publication February 26, 1937.From the Department of Internal Medicine, Hanna, Clay and Lancaster Clinic, Fargo, N. Dak. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 October 1937Volume 11, Issue 4Page: 682-684KeywordsDeliriumEncephalitisMeningitisMumps Issue Published: 1 October 1937 PDF downloadLoading ...
Respiratory occupational therapy (OT) within a community respiratory team: Referral and intervention patterns
Authors: Lynsey Wright, Jessica Callaghan, Claire Morris, Fran Dyer, Julia Bott
Journal: European Respiratory Journal (2012)

Introduction 

OT aims to maximise independence, occupational performance and improve quality of life. Respiratory OT provides a specialist functional and psychological dimension to assess and manage respiratory patients.

The psychological dimension is predominantly based on cognitive behavioural therapy principles in anxiety, depression and hyperventilation management.

Aim 

To determine the relationship between the intervention requested (whether for psychological functioning (Psy F) and/or physical functioning (Phy F)) and the intervention provided by respiratory OT.

Method 

All referrals to West Surrey respiratory OT between January and December 2011, and the reason for referral and intervention provided were recorded.

Results 

239 OT referrals were recorded in 2011. 33 of these were excluded from the analysis, as OT was not required or the patient had died.

View this table:

Intervention Requestion





View this table:

Intervention Provided





Of the 206 patients who received OT, 47 required physical and psychological intervention, however the intervention requested for 32 of these had been for psychological and 15 for physical only.

Conclusion 

We observe from the data that most referrals are for Psy F. However a noticeable proportion of these also required intervention to address Phy F (need identified at OT assessment).

Having received joint physical and mental health training, OT is well placed to identify and address the link between psychological and physical symptoms, which limit daily functioning, in this complex patient group. This supports the need for the specialist respiratory OT role.
Abnormalities of primitive myeloid progenitor cells expressing granulocyte colony-stimulating factor receptor in patients with severe congenital neutropenia
Authors: Kazuhiro Nakamura, Masao Kobayashi, Nakao Konishi, Hiroshi Kawaguchi, Shin-ichiro Miyagawa, Takashi Sato, Hidemi Toyoda, Yoshihiro Komada, Seiji Kojima, Osamu Katoh, Kazuhiro Ueda
Journal: Blood (2000)
Progression of Diabetic Retionopathy after Pancreas Transplantation
Authors: Michael R. Petersen, Andrew K. Vine, Donald C. Dafoe, Darrell A. Campbell, Robert A. Merion, Lawrence Rosenberg, Jeremiah G. Turcotte, Aaron I. Vinik, Sumer B. Pek, Jeffery Sanfield, Leslie L. Rocher, Frederic Wolf, Barbara Anderson, Vivian A. Harrison, Julie Loftin, Evelyn Dennerll, Patricia A. Prey, Sylvia A. Halloran, Maureen E. Fox, Jane A. Waskerwitz, Mary K. O'Neil, Andrew K. Vine, Mary Clifford
Journal: Ophthalmology (1990)
Attributable causes of cancers: an evidence-based approach
Authors: Paolo Boffetta, Philippe Autier, Mathieu Boniol, Maurice Tubiana, Peter Boyle
Journal: Cancer Research (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

4555 

Most commonly quoted proportions of cancers attributable to various causes derive from the estimates produced in the early 1980s by Doll and Peto. Since then, new information has accumulated on the identification of human carcinogens, the quantification of their effects and the prevalence of their exposure. We have performed a systematic review of the causes of human cancer and derived attributable risks based on results on meta- or pooled analyses and representative data on prevalence of exposure. We have used this evidence-based approach to calculate sex-specific attributable risks of cancer incidence and mortality in France in 2000. Our results on cancer incidence are presented in the table; they are similar to the results on cancer mortality. Tobacco is the main cause of cancer in France; alcohol drinking and reproductive factors are responsible for an important proportion of total cancers. The only nutritional factors responsible for a sizable number of cancers are overweight and obesity. The role of occupational exposures and pollutants appears to be smaller than previously reported. Known causes of cancer are responsible for approximately 45% of cancers in men and 35% in women. These figure are conservative and are not directly applicable to other countries. They stress stress the need for further fundamental and epidemiological research. The role of viruses and infections is probably underestimated as well as that of nutritional factors, especially during young age. They should be investigated and interactions better assessed.



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Adequacy of endobronchial ultrasound transbronchial needle aspiration samples in the sub-typing of non small cell lung cancer
Authors: Georgina Esterbrook, Sujo Anathhanam, Paul Plant
Journal: European Respiratory Journal (2012)

Introduction  The histological sub-typing of non small cell lung cancer (NSCLC) has become increasingly important due to advances in systemic therapy. There are now important differences in the treatment of squamous and non-squamous cancers. Non-squamous cancers (particularly adenocarcinomas) are also suitable for targeted therapy if the epidermal growth factor receptor (EGFR) genetic mutation is present.  Diagnosis is frequently made by fine needle aspiration from lymph node metastases.   Objectives  To analyse endobronchial ultrasound transbronchial needle aspiration (EBUS TBNA) data to establish our NSCLC not otherwise specified (NOS) rate and determine the technical success of EGFR testing.   Methods  All EBUS TBNA procedures performed at Leeds Teaching Hospitals between February 2009 and November 2011 were analysed. Data was collected on the indication, final histological diagnosis and whether EGFR mutation testing was possible.   Results  Data from 391 procedures was analysed. The indication was staging of malignancy in 345 patients and suspected non-malignant disease in 48 patients.  Malignant disease was diagnosed in 204 patients (52.2%), small cell 43, squamous cell 64, adenocarcinoma 40, adenosquamous 2, large cell 12, NSCLC NOS 31 and malignant disease of non lung primary 12.  The number of cases of NSCLC NOS was 31 of 149 NSCLCs. The NOS rate was 20.8%.  EGFR testing was requested in 36 patients. The sample was sufficient to allow testing in 32 patients (88.8%).   Conclusion  This data shows that EBUS TBNA samples are of adequate size to allow the determination of NSCLC sub-type and EGFR mutation status provided appropriate laboratory techniques are used.
Abstract 16817: Body Fatness is Associated With Carotid Intima-Media Thickness in Non-Obese Adults
Authors: Helena Podrouzkova, Peter Wohlfahrt, Virend Somers, Ling Hinshaw, Ondrej Sochor, Iftikhar Kullo, Francisco Lopez-Jimenez
Journal: Circulation (2014)

Background:  Adults with normal body mass index (BMI) but high body fat percentage have higher cardiovascular disease (CVD) risk than subjects with normal weight and low fat mass. However, the association of body fat and subclinical atherosclerosis in carotid artery in non-obese individuals has not been reported. Our aim was to test the hypothesis that even in the absence of obesity by BMI, IMT correlates with total fat and fat distribution. Additional aim was to test the possible interaction between gender, fat and IMT.   Methods:  We measured the carotid intima-media thickness (IMT) with ultrasound in three different locations (common carotid artery, bifurcation and internal carotid artery) and created a composite value averaging all IMT measurements in 135 healthy volunteers with a BMI  2  and without major CVD risk factors. Body composition was measured by dual-energy x-ray absorptiometry. We did raw and multivariate analyses adjusting for age, gender, height squared, systolic blood pressure, total cholesterol, and total muscle mass.   Results:  Age (mean ±SD) was 44 ±9 yrs, range 29-65 yrs, 56% women, 45% Whites, 44% Asians, 10% Hispanic, 1% African-American. There was no statistically significant interaction between gender, fatness and IMT. Composite IMT was 0.50 ±0.11 mm, median 0.49, IQR 0.42-0.56. Total fat and centrally distributed fat were positively associated with IMT. (Figure)   Conclusion:  In healthy, non-obese adults without major CVD risk factors, greater body fat content was independently and directly associated with higher IMT. The association between body fat and IMT appeared to be different between genders, but the study was not powered to assess gender differences.
Physicians as Citizens
Authors: Douglas L Weed, Graça M Dores
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Evaluation of two matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems for the identification of Candida species
Authors: C. Lacroix, A. Gicquel, B. Sendid, J. Meyer, I. Accoceberry, N. François, F. Morio, G. Desoubeaux, J. Chandenier, C. Kauffmann-Lacroix, C. Hennequin, J. Guitard, X. Nassif, M.-E. Bougnoux
Journal: Clinical Microbiology and Infection (2013)
Arthritis as a potential barrier to physical activity among adults with heart disease - United States, 2005 and 2007.
Authors: J. Bolen, L. Murphy, K. Greenlund, C. G. Helmick, J. Hootman, T. J. Brady, G. Langmaid, N. Keenan
Journal: MMWR Morbidity and Mortality Weekly Report (2009)