tbuckley/cabot-search huggingface dataset:
Caveolin-1 and -3 dissociations from caveolae to cytosol in the heart during aging and after myocardial infarction in rat
Authors: P Ratajczak
Journal: Cardiovascular Research (2003)

Objective: Caveolins, the structural proteins of caveolae, modulate numerous signaling pathways including Nitric Oxide (NO) production. Among the caveolin family, caveolin-1 and -3 are mainly expressed in endothelial and muscle cells, respectively. In this study, we investigate whether (i) changes in caveolin abundance and/or distribution occur during cardiac aging and failure in rat, and (ii) the process could influence NO synthase (NOS) activity. Methods: Using immunohistolabelling and Western blot approaches, expression and distribution of caveolins were analysed in adult (Ad), senescent (S-Sh) and myocardial infarction-induced failing (S-MI) hearts. NOS3/caveolin-1 interactions were evaluated by immunoprecipitation assays. Results: At the microscope level, caveolin-1 distribution in the endothelial cells was unchanged between the groups. Conversely the typical distribution of caveolin-3 in myocyte sarcolemma was dramatically altered in S-MI rats, resulting in a heterogeneous pattern throughout the septum. Total abundance of caveolin-1 and -3 remained stable whatever the group. In the fractions free of caveolae (Triton X-100 soluble), the levels of caveolin-1α and -3 increased with aging (+20%, and +104%, P<0.05 versus Ad, respectively) and were further enhanced in S-MI (+25%, +30%, P<0.05, P<0.001 versus S-Sh respectively). In these fractions, NOS3/caveolin-1α complexes increased as well. In addition, NOS activity was negatively correlated to caveolin-1 level in the cytosolic fractions. Conclusions: We demonstrate that dissociation of caveolin from caveolae is associated with aging and heart failure, the process being related to the decreased NOS activity.
The Demand for Primary Health Services in the Third World
Authors: David Hemenway
Journal: JAMA (1985)

Making services available does not ensure that they will be used; "supply" is a necessary but not sufficient condition for utilization. For example, the establishment of up-to-date government-run health clinics in less-developed countries does not guarantee that the poor will receive modern medical care. As economists, Akin et al focus on the "demand" for primary health care. Their book attempts to identify the crucial factors affecting actual consumption of available health services in the Third World. After reviewing the diverse and often noneconomic theoretical and empirical literature, the authors analyze data from the Bicol region of the Philippines. Surprisingly, there is no dearth of medical resources available in this poor rural area. The sick have a variety of options; the book tries to explain statistically their choice among self-help, traditional providers, modern private practitioners, and government clinics. The government clinics are nominally free, but there are drug prices, transportation expenses,
Molecular imaging using fluorescent lectins permits rapid endoscopic identification of dysplasia in Barrett's esophagus
Authors: Elizabeth L Bird-Lieberman, André A Neves, Pierre Lao-Sirieix, Maria O'Donovan, Marco Novelli, Laurence B Lovat, William S Eng, Lara K Mahal, Kevin M Brindle, Rebecca C Fitzgerald
Journal: Nature Medicine (2012)
RAW FOOD EXTRACTS.
Authors: B. N. TOWLE
Journal: JAMA (1884)

Read in the Section on Hygiene of American Medical Association, May, 1884. Molescott's dictum is suggestive, "Without phosphorus there is no thought;" so without nutrition there is no life. Life may be defined as an aggregation of molecules possessing the properties of respiration, circulation culation and nutrition. Each of these properties being necessary to all forms of life, the perfection of that life relies upon the completeness of the conditions such these properties depend. Either of these properties being imperfect or vitiated, the perfection of the organization is impaired, and the life jeopardized. Pure air in abundance must be supplied. A perfect circulatory system to convey the pabulum to the remotest atom of the organization, and a fluid bearing on its bosom nutritive material suited to the wants of the most delicate tissues is a uniform necessity to health and vigor. From the food the blood is fed, and from
Long survival in advanced breast cancer
Authors: Mark L. Fuerst
Journal: JAMA (1983)

Adjuvant polychemotherapy can turn the mortality tables more in favor of advanced breast cancer patients, according to reports presented at a recent symposium on innovative cancer chemotherapy held in New York City. Intensive, aggressive, postsurgical polychemotherapy, including prednisone for hormonal catabolic support, can provide a "guarantee" of an extra 2 1/2 years of life for an estimated 200,000 American women who are presently undergoing therapy for recurrent or advanced breast cancer, said Ezra M. Greenspan, MD, associate chief, Division of Medical Oncology, Mount Sinai School of Medicine, New York. Most multi-institution studies of high-risk patients with metastatic breast cancer indicate that five-year survival rarely exceeds 10% to 15%, he told the symposium. Greenspan said, "For the high-risk patient with life-threatening [disease], there is now no question of the need for aggressive polychemotherapy." Greenspan's statements were reinforced by data presented by Richard G. Cooper, MD, chief of oncology at Buffalo (NY)
The Ethics of Placebo-Controlled Trials
Authors: Tom Reynolds
Journal: Annals of Internal Medicine (2000)

Current Clinical Issues19 September 2000The Ethics of Placebo-Controlled TrialsTom ReynoldsTom ReynoldsSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-133-6-200009190-00103 SectionsAboutFull Text ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Placebo-controlled clinical trials are often considered the gold standard for evaluation of a new therapy because they are thought to provide the most definitive test of efficacy. The U.S. Food and Drug Administration (FDA), which is charged with ensuring both safety and efficacy, frequently requires pharmaceutical companies to perform placebo-controlled trials before a new drug is approved.Critics of such trials, however, believe that it is unethical to give patients a placebo when an approved therapy exists, even when the required informed consent has been provided. For support, they point to the Declaration of Helsinki, a document first ratified by ... Author, Article, and Disclosure InformationAuthors: Tom Reynolds PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetailsSee AlsoCorrection: Ethics of Placebo-Controlled Trials Metrics Cited byReferencesMagnesium and depression: a systematic reviewChallenges and opportunities: what we are learning from the clinical natalizumab experienceEthical dilemmas encountered during clinical drug trialsEthical Aspects of Placebo in Migraine ResearchThe ABC of pharmaceutical trial design: some basic principlesDeconstructing the Placebo Effect and Finding the Meaning ResponseDaniel E. Moerman, PhD and Wayne B. Jonas, MDPlacebos, Placebo Effect, and the Response to the Healing Situation: The Evolution of a ConceptCorrection: Ethics of Placebo-Controlled Trials 19 September 2000Volume 133, Issue 6Page: 491-492KeywordsBiologicsClinical trialsConflicts of interestDrug therapyDrugsEpidemiologyFood and Drug AdministrationResearch ethicsSafetyScientists ePublished: 19 September 2000 Issue Published: 19 September 2000 Copyright & PermissionsCopyright © 2000 by American College of Physicians. All Rights Reserved.Loading ...
GPs accept patients' vote on fundholding
Authors: O. Mulka
Journal: BMJ (1995)

transdermal nicotine patches before marketing them.The patients with heart disease who participated were kept under strict cardiovascular control.Many cases of angina pectoris and arrhythmias were reported, which led to patients being with- drawn from the study, being admitted to hospital, and even undergoing angioplasty.The study con- cluded that patches are safe only in patients with stable cardiovascular disease.4The pharmacological effects of nicotine (regard- less of its source) on the cardiovascular system are well known.(1) Nicotine increases blood pressure and heart rate directly, because it has sympathomimetic effects on the heart, and indirectly, because it stimulates catecholamine release.These effects increase myocardial oxygen demands, risking the development of angina pectoris due to reversible
A NEW SCALPEL WITH INTERCHANGEABLE BLADES
Authors: Paul B. Magnuson
Journal: JAMA (1925)

The scalpel illustrated and described here was developed with the idea of furnishing the surgeon a blade strong enough for all purposes and all types of tissues, and sharp enough to do its work easily. The handle (Fig. 1), which accommodates all sizes of blades, is heavy enough to give the operator a firm grip. It feels light in the hand. It has a dissecting, blunt end opposite the blade. The six types of blades (Fig. 2) furnished with this handle are somewhat different from the detachable blades now on the market. They are made heavier and therefore in dissecting, especially in heavy scar tissue, it is possible to use the blades with a side motion, making the dissection much easier and involving no danger of breaking the blade. As will be seen in Figures 3 and 4, the blade is applied in the handle and held by a little
Failure of isotretinoin to control dermatitis herpetiformis and subcorneal pustular dermatosis
Authors: A. J. Rutman, A. V. Powles, C. E. M. Griffiths, J. McFadden, L. Fry
Journal: British Journal of Dermatology (1988)

Journal Article Failure of isotretinoin to control dermatitis herpetiformis and subcorneal pustular dermatosis Get access A. J. Rutman, A. J. Rutman Department of Dermatology, St. Mary's Hospital, London Wz, U.K. Search for other works by this author on: Oxford Academic Google Scholar A. V. Powles, A. V. Powles Department of Dermatology, St. Mary's Hospital, London Wz, U.K. Search for other works by this author on: Oxford Academic Google Scholar C. E. M. Griffiths, C. E. M. Griffiths Department of Dermatology, St. Mary's Hospital, London Wz, U.K. Search for other works by this author on: Oxford Academic Google Scholar J. McFadden, J. McFadden Department of Dermatology, St. Mary's Hospital, London Wz, U.K. Search for other works by this author on: Oxford Academic Google Scholar L. Fry L. Fry Department of Dermatology, St. Mary's Hospital, London Wz, U.K. Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 119, Issue 2, 1 August 1988, Pages 270–271, https://doi.org/10.1111/j.1365-2133.1988.tb03214.x Published: 01 August 1988
Suffering among carers working in critical care can be reduced by an intensive communication strategy on end-of-life practices
Authors: J. P. Quenot, J. P. Rigaud, S. Prin, S. Barbar, A. Pavon, M. Hamet, N. Jacquiot, B. Blettery, C. Hervé, P. E. Charles, G. Moutel
Journal: Intensive Care Medicine (2011)
Central Nervous System Hemorrhage
Authors: Joshua P Klein, Robin C Ryther
Journal: New England Journal of Medicine (2009)

A 68-year-old man who had hypertension, diabetes, and atrial fibrillation and was being treated with warfarin was discovered snoring and unarousable at home. He had been in his usual state of good mental and physical health earlier in the day. There was no history of recent falls or other trauma. He was intubated by emergency medical technicians for airway protection. On physical examination, he was afebrile and his heart rate was 71 beats per minute, his blood pressure 181/100, and his oxygen saturation 100%. The score on the Glasgow Coma Scale was 3, his pupils were 8 mm in diameter . . .
Interferon first in chronic hepatitis C.
Authors: M Goggins, A Tobin
Journal: BMJ (1993)

Biological mechanisms for link EDITOR,-Frank DeStefano and colleagues report an increased risk of coronary heart disease and mortality in men with dental disease.'There is evidence that immunocompetent cells have a role in initiating and accelerating the development of atherosclerosis and myocardial infarction.In particular, activated macrophages are considered to play a pivotal part.Increased serum concentrations of neopterin are found in patients with atherosclerosis,2 and in a community based study neopterin was the best single predictor of atherosclerosis-even better than more classic variables like cholesterol, low density lipoprotein, and high density lipoprotein.3Large amounts of neopterin are released from human macrophages on stimulation with interferon y.Increased serum neopterin concentrations have been found in patients with infectious syndromes, malignancies, and autoimmune diseases.4Interestingly, among blood donors a substantial proportion with a raised serum neopterin concentration presented with dental diseases.'These data indicate that dental diseases may often lead to chronic activation of the immune system, which may render patients more sus- ceptible to atherogenesis, thus increasing the risk of myocardial infarction.It would be interesting to learn from DeStefano and colleagues whether variables of immune activation are more commonly increased in their patients with dental disease and with increased risk of myocardial infarction.
Fortnightly Review: Management of genital candidiasis
Authors: D W Denning
Journal: BMJ (1995)

<h3>Summary points</h3> Vaginal candidiasis affects about 75% of women, 40-50% having recurrent episodes Pruritus vulvae and vaginal discharge are the cardinal symptoms Candida albicans accounts for about 90% of infections and C glabrata for 5% C glabrata infections are often resistant to azoles Recurrent episodes require clinical examination, culture of swabs, and consideration of underlying disease Male partners who do not have symptoms need not be examined, have swabs taken for culture, or be treated Reduction of intestinal colonisation is of no value in preventing recurrence
Fall and rise of immunity to rubella.
Authors: N D Munro, N J Wild, S Sheppard, R W Smithells, M H Hambling
Journal: BMJ (1987)

as high a concentration as this spontaneously.Nevertheless, there was no indication of increased aggression, though she was very dramatically virilised.A wide range of ovarian tumours can secrete androgens.'Though virilisation is often severe, serum testosterone concentrations are usually increased only up to the normal male range at the most.After resection ofthe tumour she showed a dramatic response that greatly exceeded everyone's expectations.The diagnosis was delayed principally because the wrong test was carried out.This case shows that measurements of serum testosterone and androstenedione concentrations, perhaps with dehydroepiandrosterone sulphate, are very much better at excluding virilising tumours than urinary 17 ketosteroid excretion, which correlates poorly with serum androgen concentrations.2Ketosteroid excretion generally represents the weaker adrenal androgens,3 and though her testosterone concentration 11 years previously was probably much lower than it was at the time of diagnosis it would doubtless already have been considerably increased at that time.
Abstract 3085: ERα phosphorylation on Ser167 in the development of endometrial cancer
Authors: Sandra Herrero Gonzalez, Antonio Di Cristofano
Journal: Cancer Research (2012)

Abstract Endometrial carcinoma (EC) is the most common malignancy of the female genital tract. Type I (endometrioid) EC is associated with elevated estrogen signaling. One of the earliest genetic events in human type I EC is loss of the tumor suppressor gene PTEN. Loss of PTEN allows unrestrained activation of the AKT kinase, which, in turn, regulates such key processes as proliferation, survival, cell size, and mRNA translation. We have previously shown, using a Pten+/− mouse model, that the neoplastic lesions develop even in the absence of estrogen, suggesting that there might exist a functional and physiological link between loss of Pten and hormone-independent increased estrogen receptor activity. Previous studies have suggested that AKT can phosphorylate and activate estrogen receptor alpha (ERα) at Ser167, promoting the activation of this nuclear receptor both in vivo and in vitro, even in the absence of ligand. In order to define in vivo the role of ERα phosphorylation on Ser167 in normal endometrial physiology and during neoplastic transformation, we have designed a knock-in approach to introduce the S167A mutation in the mouse germline (ERαA/A). These mice are viable and fertile. We bred ERαA/A mice to Pten+/− mice to test whether ERα phosphorylation on Ser167 is required for the development of endometrial hyperplasia and carcinoma. We found that, at the age of 6 months, the uterus weight of ERαA/A/Pten+/− mice was significantly reduced compared to that of Pten+/− mice. To elucidate whether inhibition of ERα phosphorylation on Ser167 affects cell cycle progression, we analyzed BrdU incorporation in primary cultures of control and ERαA/A endometrial epithelial cells, and found that impairment of ERα phosphorylation on Ser167 leads to a significant reduction of the number of actively proliferating cells. In order to understand the molecular mechanisms responsible for this cell cycle impairment, we analyzed by real-time PCR the expression levels of cell cycle-related genes in the uterus of control and ERαA/A mice. Surprisingly, our results indicate that many genes related with the G2/M phase of the cell cycle, like Ccna2 or Ccnb1, and spindle formation, like Plk1 or Tpx2, are deregulated in ERαA/A uteri, compared to wild-type controls. In conclusion, our results indicate that ERα phosphorylation on Ser167 is an obligatory pathway for the development of endometrial lesions consequent to loss of Pten in endometrial cancer, at least in part by affecting the expression of cell cycle genes. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3085. doi:1538-7445.AM2012-3085
Accelerated Achilles tendon healing by PDGF gene delivery with mesoporous silica nanoparticles
Authors: Arnaud Suwalski, Hinda Dabboue, Anthony Delalande, Sabine F. Bensamoun, Francis Canon, Patrick Midoux, Gérard Saillant, David Klatzmann, Jean-Paul Salvetat, Chantal Pichon
Journal: Biomaterials (2010)
1103 Expression and regulation of adenylyl cyclase isoforms (ACS) in rat cholangiocytes; effects of lipopolysaccaride (LPS) and α-napthylisothiocyanate (ANIT) treatment
Authors: S MELERO, M STRAZZABOSCO, C SPIRLI, R FIOROTTO, S GLASER, H FRANCIS, G ALPINI
Journal: Hepatology (2003)
Theophylline Toxicity and the Beta-Adrenergic System
Authors: THOMAS J. IBERTI
Journal: Annals of Internal Medicine (1985)

Letters and Corrections1 December 1985Theophylline Toxicity and the Beta-Adrenergic SystemTHOMAS J. IBERTI, M.D., ERNEST BENJAMIN, M.D.THOMAS J. IBERTI, M.D.Search for more papers by this author, ERNEST BENJAMIN, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-103-6-957_3 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: In the study by Kearney and colleagues (1) concerning theophylline toxicity and the beta-adrenergic system, certain points of the experimental protocol are not immediately clear to us. The authors appear to have studied only one animal in each protocol. If that is the case we have serious reservations about the validity of this study. Tremendous variations can occur from one dog to the next that one-dog group studies cannot take into consideration. For example, these variations may account for the fact that the basal heart rate was 210/minute in Protocol 1 and 126/minute in Protocol 2 and...Reference1. KEARNEYMANOGUERRACURTISZIEGLER TAGM. Theophylline toxicity and the beta-adrenergic system. Ann Intern Med. 1985;102:766-9. LinkGoogle Scholar1. KEARNEYMANOGUERRACURTISZIEGLER TAGM. Theophylline toxicity and the beta-adrenergic system. Ann Intern Med. 1985;102;766-9. LinkGoogle Scholar2. KEARNEYMCELWEEDAILEY TNJ. Theophylline toxicity in a canine model: role of the beta-adrenergic system [Abstract 28]. Vet Hum Toxicol. 1985:27. (In press). Google Scholar3. BIBERSTEINZIEGLERWARD MMD. Use of beta-blockade and hemoperfusion for acute theophylline poisoning. West J Med. 1984;141:485-90. MedlineGoogle Scholar4. HALLDOBSONDALTONGHIGNONEPENNER KKJMS. Metabolic abnormalities associated with intentional theophylline overdose. Ann Intern Med. 1984;101:457-62. LinkGoogle Scholar5. WALSONJONES PP. Theophylline overdose treated with propranolol [Abstract H-2]. Vet Hum Toxicol. 1983:25(suppl 1):69. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Mount Sinai Medical Center New York, NY 10029 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited ByThe assessment and treatment of theophylline poisoning 1 December 1985Volume 103, Issue 6_Part_1Page: 957-958KeywordsHeart rateTheophylline toxicity Issue Published: 1 December 1985 PDF DownloadLoading ...
Research in progress—electronic patient records: a new era
Authors: D Peckham, P Whitaker, H White
Journal: Thorax (2014)

Clinical information systems and electronic records are starting to appear in secondary care and herald new potentials for improving health provision and capturing high quality data. In 2006, we set up a program to develop electronic patient records (EPR) for chronic disease using Cystic Fibrosis (CF) as our initial model. Seven years on we are now exploring the real time clinical data to identify risks, trends and outcomes in chronic disease management. We are also working to establish new models of integration and to connect information between the client and all areas of health care.
Regulation of cholesterol and bile acid homeostasis by the cholesterol 7α-hydroxylase/steroid response element-binding protein 2/microRNA-33a axis in mice
Authors: Tiangang Li, Jessica M. Francl, Shannon Boehme, John Y. L. Chiang
Journal: Hepatology (2013)

Bile acid synthesis not only produces physiological detergents required for intestinal nutrient absorption, but also plays a critical role in regulating hepatic and whole-body metabolic homeostasis. We recently reported that overexpression of cholesterol 7α-hydroxylase (CYP7A1) in the liver resulted in improved metabolic homeostasis in Cyp7a1 transgenic ( Cyp7a1 -tg) mice. This study further investigated the molecular links between bile acid metabolism and lipid homeostasis. Microarray gene profiling revealed that CYP7A1 overexpression led to marked activation of the steroid response element-binding protein 2 (SREBP2)-regulated cholesterol metabolic network and absence of bile acid repression of lipogenic gene expression in livers of Cyp7a1 -tg mice. Interestingly, Cyp7a1 -tg mice showed significantly elevated hepatic cholesterol synthesis rates, but reduced hepatic fatty acid synthesis rates, which was accompanied by increased 14 C-glucose-derived acetyl-coenzyme A incorporation into sterols for fecal excretion. Induction of SREBP2 also coinduces intronic microRNA-33a (miR-33a) in the SREBP2 gene in Cyp7a1 -tg mice. Overexpression of miR-33a in the liver resulted in decreased bile acid pool, increased hepatic cholesterol content, and lowered serum cholesterol in mice. Conclusion : This study suggests that a CYP7A1/SREBP2/miR-33a axis plays a critical role in regulation of hepatic cholesterol, bile acid, and fatty acid synthesis. Antagonism of miR-33a may be a potential strategy to increase bile acid synthesis to maintain lipid homeostasis and prevent nonalcoholic fatty liver disease, diabetes, and obesity. (Hepatology 2013;53:1111–1121)