tbuckley/cabot-search huggingface dataset:
Cyclic strain-mediated regulation of endothelial matrix metalloproteinase-2 expression and activity
Authors: N VONOFFENBERGSWEENEY
Journal: Cardiovascular Research (2004)

To investigate the role of cyclic strain in controlling matrix metalloproteinase-2 (MMP-2) expression and activity in endothelial cells (ECs) in vitro.A Flexercell Tension Plus FX-4000T system was used to apply a physiological level of equibiaxial cyclic strain (0-10% strain, 60 cycles/min, 0-24 h, cardiac waveform) to bovine aortic endothelial cells (BAECs). Cells and conditioned media were harvested for analysis of MMP-2/9 expression and activity (pro and active) using reverse-transcriptase polymerase chain reaction (RT-PCR), Western blotting and zymography techniques.Cyclic strain significantly increased MMP-2 expression and activity force- and time-dependently. Pretreatment with Gialpha-protein inhibitors, pertussis toxin (PTX) and NF023, transient expression of inhibitory mutants of Gialpha-subunits, or pretreatment with RGD peptides to block RGD-dependent integrin signaling failed to attenuate strain-induced increases in MMP-2 expression in BAECs. In contrast, inhibition of Gbetagamma-signaling with betaArk-ct or tyrosine kinase blockade with genistein reduced strain-induced MMP-2 expression while concomitantly inhibiting strain-induced p38 and ERK activity in these cells. Pretreatment with PD169316 and PD98059 to selectively inhibit p38 and ERK activity, respectively, also resulted in a significant inhibition of the strain-induced MMP-2 response. Finally, inhibition of the adaptor protein, Shc, (via Shc-SH2 transfection) resulted in a significant decrease in strain-induced MMP-2 activity concomitant with a reduction in ERK activity in BAECs.Cyclic strain stimulates MMP-2 expression, in part, by stimulating both p38- and ERK-dependent pathways through activation of Gbetagamma and tyrosine kinase in BAECs.
MALARIA THERAPY IN ASYMPTOMATIC NEUROSYPHILIS
Authors: Paul A. O'leary
Journal: Annals of Internal Medicine (1934)

Article1 June 1934MALARIA THERAPY IN ASYMPTOMATIC NEUROSYPHILISPAUL A. O'LEARY, M.D., F.A.C.P.PAUL A. O'LEARY, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-7-12-1513 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThis is a report of the results of treatment with malaria of a group of patients who had asymptomatic neurosyphilis. Under the classification of asymptomatic neurosyphilis are included those cases in which tests of spinal fluid are positive, but in which there are neither signs nor symptoms of syphilitic involvement of the central nervous system. The length of time the patient has had syphilis is not a factor in the incidence of asymptomatic neurosyphilis, for its presence may be demonstrated any time after the infection has been acquired. It has been known for some time that the nervous system becomes...Bibliography1. CLARKPARRANCOLEMOOREO'LEARYSTOKESWILE TTHNJEPAJHUJ: Coöperative clinical studies in the treatment of syphilis. Reprint No. 41, from Venereal Disease Information, U. S. Public Health Service, 1932, xiii, 13-14. Google Scholar2. MOORE JE: The modern treatment of syphilis, 1933, Charles C. Thomas Company, Baltimore. Google Scholar3. O'LEARY PA: Treatment of neurosyphilis by malaria; serologic results and a comparison with treatment by typhoid vaccine, Jr. Am. Med. Assoc., 1928, xci, 543-544. CrossrefGoogle Scholar4. O'LEARY PA: Treatment by malaria in asymptomatic neurosyphilis, Jr. Am. Med. Assoc., 1931, xcvii, 1585-1586. CrossrefGoogle Scholar5. O'LEARYRUBENSTEIN PAMW: A study of acquired syphilis in family groups, Minnesota Med., 1928, xi, 406-409. Google Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Rochester, Minnesota*Read before the American College of Physicians, Chicago, Illinois, April 16, 1934.From the Section on Dermatology and Syphilology, The Mayo Clinic. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byFever Therapy in Diseases of the Nervous System 1 June 1934Volume 7, Issue 12Page: 1513-1522KeywordsCentral nervous systemDermatologyMalariaNervous systemSigns and symptomsSyphilis ePublished: 1 December 2008 Issue Published: 1 June 1934 PDF downloadLoading ...
Fatal cytomegalovirus pneumonia in patients with haematological malignancies: an autopsy-based case–control study
Authors: H.A. Torres, E. Aguilera, A. Safdar, N. Rohatgi, I.I. Raad, C. Sepulveda, M. Luna, D.P. Kontoyiannis, R.F. Chemaly
Journal: Clinical Microbiology and Infection (2008)
The Fanconi anaemia group G gene FANCG is identical with XRCC9
Authors: Johan P. de Winter, Quinten Waisfisz, Martin A. Rooimans, Carola G.M. van Berkel, Lucine Bosnoyan-Collins, Noa Alon, Madeleine Carreau, Olaf Bender, Ilja Demuth, Detlev Schindler, Jan C. Pronk, Fré Arwert, Holger Hoehn, Martin Digweed, Manuel Buchwald, Hans Joenje
Journal: Nature Genetics (1998)
"Slow Sodium": Double-Edged Drug
Authors: Karl D. Nolph
Journal: Annals of Internal Medicine (1972)

Editorial Notes1 May 1972"Slow Sodium": Double-Edged DrugKARL D. NOLPH, M.D., F.A.C.P.KARL D. NOLPH, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-76-5-825_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptA recent British paper (1) has described studies of Slow Sodium®*, a tablet preparation of sodium chloride in a wax-sponge formulation, which can be ingested rapidly with minimal gastrointestinal disturbance, presumably because of slow release of electrolytes from the tablets. Each 600-mg tablet contains 10 mmoles [10 mEq] of sodium and is totally absorbed over 6 hours. As much as 200 mmoles [200 mEq] per 10 minutes and 500 mmoles [500 mEq] per day of sodium can be tolerated orally. Slow Sodium was shown to prevent sodium depletion due to salt-losing nephritis, unwanted hemodialysis ultrafiltration, and sweat losses with strenuous...References1. CLARKSONCURTISJEWKES EJR: Slow sodium: an oral slowly released sodium chloride preparation. Br Med J 3:604-607, 1971 CrossrefMedlineGoogle Scholar2. NOLPHSCHRIER KR: Sodium, potassium, and water metabolism in the syndrome of inappropriate antidiuretic hormone syndrome. Am J Med 49:534-545, 1970 CrossrefMedlineGoogle Scholar3. NOLPHSTOLTZCARTER KMC: Factors affecting the composition of ultrafiltrate from hemodialysis coils. Trans Am Soc Artif Intern Organs 16:495-501, 1970 MedlineGoogle Scholar4. NOLPHWHITCOMBSCHRIER KMR: Mechanisms for inefficient peritoneal dialysis in acute renal failure associated with heat stress and exercise. Ann Intern Med 71:317-326, 1969 LinkGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Division of Nephrology Department of Medicine University of Missouri Medical Center Columbia, Mo.*A Ciba preparation, not currently available in the United States. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 May 1972Volume 76, Issue 5Page: 825-825KeywordsDrugsNephritisSaltsSodiumSodium chlorideSweatUltrafiltration ePublished: 1 December 2008 Issue Published: 1 May 1972 PDF downloadLoading ...
Abstract 16166: Validation of a of Self-Reported Measure of Physical Capacity in the Context of Chronic Cardiovascular and Pulmonary Diseases
Authors: Maria-Cecilia Gallani, Paul Poirier, Michel Doré, Steeve Provencher, Camille Gagné, Céline Gélinas, Sarah-Maude Caron-Cantin, Jonathan Myers
Journal: Circulation (2014)

Background: The assessment of physical capacity (PC) is relevant to estimate prognosis and guide therapeutics for patients with chronic cardiopulmonary disease. The Veterans Specific Activity Questionnaire (VSAQ) is a self-reported tool designed to assess perceived physical capacity in METs according to a given patient’s level of physical activity limited by symptoms. The purpose of this study was to examine the validity of the score of the French-Canadian version of the VSAQ among patients suffering from cardiovascular symptoms and chronic pulmonary diseases. Methods: A total of 140 subjects referred for treadmill testing (TT group) and 134 patients with pulmonary hypertension or interstitial lung disease (PD group) were enrolled. Criterion validation was verified in the TT group by level of agreement (Bland-Altman coefficient) between the VSAQ and the workload estimated by the gold standard to assess PC, the maximal treadmill test (MTT). Convergent validation was tested using correlations between the VSAQ score and physical capacity (MTT and 6-min walking test - 6MWT); practice of physical activity (Godin-Shephard Leisure-Time Physical Activity Questionnaire-GSLTPAQ); pulmonary function (forced vital capacity- FVC) and quality of life (Minnesota living with Heart Failure Questionnaire-LHFQ and SF-12). Partial correlation coefficients were adjusted for sex, age, BMI and education. Results: The mean bias between VSAQ and estimated METs was -1.54 with limits of agreement (CI 95%) of 3.71 to -6.79 METs, being constant around all MET levels. Significant correlations (p &lt;0.05) between the VSAQ score in the TT sample were 0.65 with MTT, 0.20 with the GSLTPAQ; -0.36; -0.39 and -0.29 with the LHFQ total score, physical and emotional domains. Significant correlations (p &lt;0.05) for the PD sample were: 0.58 with 6MWT; 0.43 with FVC (interstitial lung disease only); 0.31 with GSLTPAQ; and 0.65 and 0.24 with SF-12 physical and emotional components. Conclusion: The VSAQ French-Canadian version demonstrated acceptable criterion and convergent validation among two different groups of chronic disease patients. Its utilization by the nurse during routine clinical follow-up of these patients could be helpful to guide functional evaluations and interventions.
CONGENITAL HYPERPLASIA OF THE ADRENALS IN THE FEMALE (FEMALE PSEUDOHERMAPHRODISM): THE REPORT OF A CASE TREATED WITH CORTISONE AND ESTROGEN
Authors: J R TOBIN
Journal: Annals of Internal Medicine (1954)

Case Reports1 April 1954CONGENITAL HYPERPLASIA OF THE ADRENALS IN THE FEMALE (FEMALE PSEUDOHERMAPHRODISM): THE REPORT OF A CASE TREATED WITH CORTISONE AND ESTROGENJOHN R. TOBIN JR., M.D.JOHN R. TOBIN JR., M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-40-4-797 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptOf the relatively uncommon symptom complexes which comprise the adrenogenital syndrome, female pseudohermaphrodism is the most frequently encountered. An almost uniform abnormality of sex differentiation characterizes female pseudohermaphrodism and suggests that the excessive production of androgen by hyperplastic adrenals begins in the third to fifth month of embryonic development.1, aAt birth these infants have ovaries, their müllerian duct system has undergone normal female development to form fallopian tubes, uterus and vagina, and the wolffian ducts have disappeared; but the genital duct continues to open into the urethra, forming a persistent urogenital sinus as in the male. After birth, rapid...Bibliography1. Wilkins L: The diagnosis and treatment of endocrine disorders in childhood and adolescence, 1950, Charles C Thomas, Springfield, Illinois, (a) p. 222, (b) Chapter XV, figure 3. Google Scholar2. WilkinsGardnerCriglerSilvermanMigeon LLIJFSHCJ (a) : Further studies on the treatment of congenital adrenal hyperplasia with cortisone. I. Comparison of oral and intramuscular administration of cortisone, with a note on the suppressive action of compounds F and B on the adrenal, J. Clin. Endocrinol. 12: 257, 1952. (b) Wilkins, L., Crigler, J. F., Jr., Silverman, S. H., Gardner, L. I., and Migeon, C. J.: Further studies on the treatment of congenital adrenal hyperplasia with cortisone. II. The effects of cortisone on sexual and somatic development, with an hypothesis concerning the mechanism of feminization, J. Clin. Endocrinol. 12: 277, 1952. CrossrefGoogle Scholar3. DreketerPearsonBartczakMcGavak IJSETH: A rapid method for the determination of total urinary 17-ketosteroids, J. Clin. Endocrinol. 8: 295, 1948. Google Scholar4. KelleyElyRaile VCRSRB: Metabolic studies in patients with congenital adrenal hyperplasia. Effects of cortisone therapy, J. Clin. Endocrinol. 12: 1140, 1952. CrossrefGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Spokane, Washington*Received for publication August 19, 1953.From the Department of Medicine of the Rockwood Clinic, Spokane, Washington. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 April 1954Volume 40, Issue 4Page: 797-803KeywordsAndrogensBirthEstrogensGenital anatomyInfantsOvariesUterusVagina ePublished: 1 December 2008 Issue Published: 1 April 1954 PDF downloadLoading ...
537. Further Characterisation of Myo-Neurospheres
Authors: Simon P. Quenneville, Marie-Eve Ducharme, Joel Rousseau, Alain Garnier, Jacques P. Tremblay
Journal: Molecular Therapy (2004)

Adult stem cells have been isolated from several tissues like bone marrow, brain and skin. We report the isolation and characterisation of neuronal precursor cells, potentially stem cells, from primate and mouse adult muscle and their differentiation in neuronal cells. These cells could be an excellent alternative source of neuronal cells for a therapeutic approach for neurodegenerative diseases. Following an enzymatic dissociation of the tissue, cells are growing in suspension in a proliferation medium. In these conditions, neurosphere-like cells (stated myo-neurospheres) appear after 7 to 10 days. Mouse spheres were further characterised for different surface markers, like CD34, CD90, CD45 and Sca-1. Moreover, expression of different neural crest markers (p75, Snail, Slug and Twist) were detected in the proliferated cells. To induce neuronal differentiation, cells were plated on poly-D-lysine/gelatine coated petri dishes in a differentiation medium for 12 days. The differentiated cells were characterized by immunostaining for two neuronal markers (beta-III- tubulin and neurofilament M), for glial cells marker (glial cells fibrillary acidic protein) and for a myoblast marker (desmin). 15–20% of differentiated cells are expressing neuronal markers. Moreover, all differentiated neuron-like cells were negative for desmin. Interestingly, in spheres derived from mouse tissue, the expression of the limiting enzyme for the synthesis of GABA was detected in the differentiated cells only. In conclusion, we were able to isolate neural precursors from adult muscle tissue and differentiate them into neuron-like cells. These cells could be used as an autologous source of cell for neurodegenerative disease.
Evaluating the feasibility of crisis homes: an application of the matrix model.
Authors: Richard Warner
Journal: World Psychiatry (2002)

Thornicroft and Tansella emphasize some important elements in the proper planning of mental health services - finding the proper blend of community and hospital-based components, assessing local needs, taking account of economic factors, involving consumers and non-professionals in programme planning and service delivery, developing small-scale alternatives to hospital for both acute and long-term care, and being flexible enough to respond to changing circumstances. They arrive at these accurate conclusions by applying a matrix model for assessing service needs at the national, local and individual level. Part of the value of their paper lies in the description of their analytic model. I will add a further specific example of the applicability of the matrix model to service reform. The use of crisis homes provides such an example.

Under the crisis home model, acutely disturbed adults are placed in short-term foster-family homes and treated by a mobile psychiatric team. The first programme of this type was developed by Polak and colleagues (1) at Southwest Denver Mental Health Center in Colorado, during the 1970s, where it decreased the annual use of adult hospital beds to 1 per 100,000 population. Similar systems are now in operation at Dane County Mental Health Center in Madison, Wisconsin (2) and elsewhere. These programmes provide care to a variety of people in crisis, most of whom would otherwise have spent time in hospital. Many of these clients suffer from acute psychotic illness and some are actively suicidal. Violence and safety are almost never a problem, in part because of careful selection of appropriate clients and in part because clients feel pleased to be invited into another person's home and try to behave with the courtesy of house guests. For this reason, people with severe personality disorders and with psychotic illness behave better in a crisis home than they would in a hospital ward.

Using Thornicroft and Tansella's matrix model to analyse the viability and success of such a programme in a new locale, we would consider the following factors.
Economics notes: Cost effectiveness calculations and sample size
Authors: D. J Torgerson
Journal: BMJ (2000)

This is one of an occasional series of notes on economics 

Clinical trials should be large enough to detect a clinically important difference between two treatments. Yet a clinically important difference is often difficult to define and debatable. This difficulty may explain why so few published trials report the clinical reasoning underlying their sample size selection. Furthermore, clinical investigators are often suspected of approaching sample size calculations logistically rather than clinically: they estimate the number of patients who can be recruited into a trial and then ask a statistician to justify the sample size by calculating the “detectable” difference implied by the number of recruitable patients.1 Including economic criteria to aid sample size determination for clinical trials might improve the rigour of sample size selection. 2 3

Consider a recent randomised trial comparing the effectiveness of hysterectomy with hysteroscopic surgery for treating menorrhagia. 4 5 The trial found that hysteroscopic surgery …
216. Enhancement of Gene Transfection Both In Vitro and In Vivo Using Polymer Conjugated HVJ-Envelope Vector
Authors: Hidetoshi Mima, Ryuji Tomoshige, Yasuhiko Tabata, Seiji Yamamoto, Susumu Ito, Katsuto Tamai, Yasufumi Kaneda
Journal: Molecular Therapy (2005)

Vector development is critical for the advancement of human gene therapy. However, the use of viral vectors raises many safety concerns and most non-viral methods are less efficient for gene transfer. One of the breakthroughs in vector technology is the combination of the vector with various polymers. HVJ (hemagglutinating virus of Japan) envelope vector (HVJ-E) has been developed as a versatile gene transfer vector. In this study, we combined HVJ-E with cationized gelatin(CG) to make it a more powerful tool and assessed its transfection efficiency in vitro and in vivo. CG is one of the most suitable polymers for the complex formation with HVJ-E because of the positive charge and biocompatibility. As shown in Fig. 1, we combined it with HVJ-E and used it in vitro and in vivo experiments. In addition, we investigated the mechanism of the gene transfer by means of the inhibition of fusion or endocytosis. The combination of both protamine sulfate(PS) and CG with HVJ-E, referred to as PS-CG-HVJ-E, further enhanced the in vitro transfection efficiency. In CT26 cells, the luciferase gene expression of PS-CG-HVJ-E was approximately 10 times higher than that of the combination of PS with HVJ-E or the combination of CG with HVJ-E, referred to as PS-HVJ-E or CG-HVJ-E, respectively. In case of in vivo study, we tried intravenous administration at first. In conclusion, the luciferase gene expression in liver mediated by intravenous administration of CG-HVJ-E was much higher than the luciferase gene expression mediated by PS-HVJ-E or PS-CG-HVJ-E and approximately 100 times higher than that mediated by HVJ-E alone. Next, we tried intraperitoneal administration of CG-HVJ-E containing luciferase gene to the peritoneal disseminated colon tumor model mice. As shown in Fig. 2, the luciferase gene expression was specifically detected at tumor nodules. Thus, cationized gelatin-conjugated HVJ-E enhanced gene transfection efficiency both in vitro and in vivo. These results suggest that the conjugation with low molecular weight cationized gelatin may increase in vivo transfection efficiency of various recombinant viral vectors such as retrovirus, herpes virus and lentivirus vector.
The role of bone marrow biopsy and <scp>FDG‐PET/CT</scp> in identifying bone marrow infiltration in the initial diagnosis of high grade non‐<scp>H</scp>odgkin <scp>B</scp>‐cell lymphoma and <scp>H</scp>odgkin lymphoma. accuracy in a multicenter series of 372 patients
Authors: Tzu‐Hua Chen‐Liang, Taida Martin‐Santos, Andres Jerez, Leonor Senent, Maria Teresa Orero, Maria Jose Remigia, Begoña Muiña, Marta Romera, Hermogenes Fernandez–Muñoz, Jose M. Raya, Marta Fernandez‐Gonzalez, Aima Lancharro, Carolina Villegas, Juan Carlos Herrera, Laura Frutos, Jose Luis Navarro, Jon Uña, Carolina Igua, Raquel Sanchez‐Vaño, Maria del Puig Cozar, Jose Contreras, Jose Javier Sanchez‐Blanco, Elena Perez‐Ceballos, Francisco Jose Ortuño
Journal: American Journal of Hematology (2015)

Bone marrow infiltration (BMI), categorized as an extra-nodal site, affects stage and is associated with poor prognosis in newly diagnosed lymphoma patients. We have evaluated the accuracy of PET/CT and bone marrow biopsy (BMB) to assess BMI in 372 lymphoma patients [140 Hodgkin Lymphoma (HL) and 232 High Grade B-cell non-Hodgkin Lymphoma (HG B-NHL), among them 155 Diffuse Large B-Cell Lymphoma (DLCL)]. For HL cases, and taking into account PET/CT, sensitivity, negative predictive value (NPV) and accuracy were 96.7, 99.3, and 99.3% while those of BMB were 32.3, 83.8, and 85%, respectively. For HG B-NHL and considering PET/CT, sensitivity, NPV, and accuracy were 52.7, 81.7, and 84.1%, while those of BMB were 77.6, 90.2, and 90.7%, respectively. In the HG B-NHL group, 25 patients would have been under-staged without BMB. These results lead us to recommend PET/CT and the avoidance of BMB to assess BMI in HL. In the case of HG B-NHL, bone marrow status should be assessed firstly by means of PET/CT; only in either focal or diffuse PET/CT with low borderline SUV max values or in negative cases, should BMB be carried out afterwards. In the HG B-NHL setting and at the present moment, both techniques are complementary.