tbuckley/cabot-search huggingface dataset:
Callus formation is associated with hyperproliferation and incomplete differentiation of keratinocytes, and increased expression of adhesion molecules
Authors: S.H. Kim, S. Kim, H.I. Choi, Y.J. Choi, Y.S. Lee, K.C. Sohn, Y. Lee, C.D. Kim, T.J. Yoon, J.H. Lee, Y.H. Lee
Journal: British Journal of Dermatology (2010)

Journal Article Callus formation is associated with hyperproliferation and incomplete differentiation of keratinocytes, and increased expression of adhesion molecules Get access S.H. Kim, S.H. Kim Departments of Anatomy Search for other works by this author on: Oxford Academic Google Scholar S. Kim, S. Kim Departments of Anatomy Search for other works by this author on: Oxford Academic Google Scholar H.I. Choi, H.I. Choi Departments of Anatomy Search for other works by this author on: Oxford Academic Google Scholar Y.J. Choi, Y.J. Choi Departments of Anatomy Search for other works by this author on: Oxford Academic Google Scholar Y.S. Lee, Y.S. Lee Departments of Anatomy Search for other works by this author on: Oxford Academic Google Scholar K.C. Sohn, K.C. Sohn Dermatology, Research Institute for Medical Sciences, School of Medicine, Chungnam National University, 55 Munhwa‐ro, Daejeon 301‐747, Korea Search for other works by this author on: Oxford Academic Google Scholar Y. Lee, Y. Lee Dermatology, Research Institute for Medical Sciences, School of Medicine, Chungnam National University, 55 Munhwa‐ro, Daejeon 301‐747, Korea Search for other works by this author on: Oxford Academic Google Scholar C.D. Kim, C.D. Kim Dermatology, Research Institute for Medical Sciences, School of Medicine, Chungnam National University, 55 Munhwa‐ro, Daejeon 301‐747, Korea Search for other works by this author on: Oxford Academic Google Scholar T.J. Yoon, T.J. Yoon Department of Dermatology, School of Medicine, Gyeongsang National University, Jinju, Korea Search for other works by this author on: Oxford Academic Google Scholar J.H. Lee, J.H. Lee Dermatology, Research Institute for Medical Sciences, School of Medicine, Chungnam National University, 55 Munhwa‐ro, Daejeon 301‐747, Korea Search for other works by this author on: Oxford Academic Google Scholar ... Show more Y.H. Lee Y.H. Lee Departments of Anatomy Young Ho Lee. E‐mail: yhlee@cnu.ac.kr Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 163, Issue 3, 1 September 2010, Pages 495–501, https://doi.org/10.1111/j.1365-2133.2010.09842.x Published: 01 September 2010
Shorter Life Span for Cloned Mice
Authors: Joan Stephenson
Journal: JAMA (2002)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Novel mutation detection method for tumor samples grossly contaminated with normal tissue
Authors: Teri Plona, Kris Pike, Anne Book, Claudia Stewart, Shirley Tsang, Zhonghe Sun, Ester Rozenblum, David Munroe
Journal: Clinical Cancer Research (2006)

A109   For diagnostic purposes, mutation detection in cancer is difficult due to the presence of contaminating normal tissue infiltrated throughout the tumor tissue. Traditional PCR and sequencing methods are labor intensive and time consuming. Therefore, we have developed an assay using a nanochip array platform to find specific mutations in cancer-related genes. An amplicon-down method was applied. That is, the DNA sample was amplified with a biotin labeled primer and the resulting target amplicon was electronically addressed to a streptavidin membrane. This method is based on differences in melting temperatures. The data is collected either at a specific Tm, or during a series of temperature increments to create a melting curve. As proof of principle, we screened codon 12 of the K- ras  gene, which is commonly mutated in several tumor types. The analysis was done on formalin fixed, paraffin-embedded tumor samples that showed various degrees of contaminating normal tissue. We screened 233 patient samples corresponding to various cancer types. These included colon, rectum, pancreas, lymph node, lung, spleen, liver, stomach, and thyroid cancers. We were able to detect mutations in samples that contained as little as 20% tumor cells. In addition, the sensitivity was 100% and the specificity was nearly 100%. These data agreed 100% with PCR enrichment and sequencing results that were performed in parallel. Moreover, the method presented here was even more sensitive in some instances. For example, we were able to detect very low amounts of mutant allele that was not detected by traditional sequencing due to background noise. In conclusion, we found this test to be extremely accurate, reproducible, fast, and due to its versatility, generally applicable to any mutation screening. Furthermore, in a therapeutic setting, it could provide an effective selection criterion for a patient treatment regimen.
The Divine Handiwork: Evolution and the Wonder of Life
Authors: Owen Gingerich
Journal: Circulation (2010)

The title derives from the 16th-century astronomer Nicolaus Copernicus, who at the end of his wonderful cosmological chapter exclaimed, “So vast, without any question, is the divine handiwork of the almighty Creator.”  But Copernicus scarcely had a clue about the true vastness of the heavens, something that has become understood only in the 20th century.  And this change from the closed world of the middle ages to the near infinite universe of today has forced a change in the way we look at our sacred scriptures, and in particular the book of Genesis with its vivid story of Adam and Eve.  The story the tree of good and evil is a powerful metaphor about the origin of conscience and becoming human.  With the understanding of right and wrong comes responsibility, and the ability to make wrong choices.  What is important is to see the Bible taking as its starting point the emergence of our humanness, the origin of humanity.  If we keep this as a central issue in our understanding of why we are special in the cosmos, then we should have no trouble in accepting evolution as a major explanatory scheme in comprehending the natural world.
Cardiovascular Outcomes and Antihypertensive Drug Treatment in Older Women
Authors: Jan A Staessen, Lutgarde Thijs, Jiguang Wang, Yan Li
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
In Defense of the Stethoscope
Authors: James B. Herrick
Journal: Annals of Internal Medicine (1930)

Article1 August 1930In Defense of the StethoscopeJAMES B. HERRICKJAMES B. HERRICKSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-4-2-113 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThere is a tendency nowadays to attack the stethoscope. It is criticized in print, in addresses in medical societies and in conversation between doctors. As a result it is losing caste as an instrument of diagnosis. Moreover, its loss of prestige carries with it a lessened appreciation of physical diagnosis in general, once a most highly regarded feature in medicine.The charges against the stethoscope are two: that it is antiquated, its place taken by other methods especially by the x-ray, and that it does more harm than good because its findings are so often misinterpreted.As to the first... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Chicago*Read before the American College of Physicians, February 12, 1930, Minneapolis, Minn. Nextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byIn defense of the stethoscope and the bedside 1 August 1930Volume 4, Issue 2Page: 113-116 ePublished: 1 December 2008 Issue Published: 1 August 1930 PDF downloadLoading ...
Retaining Smallpox Stocks
Authors: Joan Stephenson
Journal: JAMA (2002)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
The novel anthracycline annamycin is not affected by P-glycoprotein- related multidrug resistance: comparison with idarubicin and doxorubicin in HL-60 leukemia cell lines
Authors: U Consoli, W Priebe, YH Ling, R Mahadevia, M Griffin, S Zhao, R Perez-Soler, M Andreeff
Journal: Blood (1996)
Abstract 2674: Stroma biology identifies heparins as differentiating agents in neuroblastoma
Authors: Erik H. Knelson, Angela L. Gaviglio, Jasmine C. Nee, Mark D. Starr, Andrew B. Nixon, Stephen G. Marcus, Gerard C. Blobe
Journal: Cancer Research (2014)

Abstract The neuroblastoma tumor stroma is thought to suppress neuroblast growth via release of soluble differentiating factors. Here we identify these factors and design a novel therapy based on their mechanism of action. We show that expression of heparan-sulfate proteoglycan co-receptors (HSPGs), including TβRIII, GPC1, GPC3, SDC3, and SDC4, is decreased in neuroblasts, localized to the stroma and correlates with improved prognosis. Treatment with soluble HSPGs promoted neuroblast differentiation via FGFR1, Erk, and Id1. HSPGs also enhanced differentiation from FGF2 released by the stroma. The anticoagulant heparin had similar differentiating effects, leading to decreased neuroblast proliferation. Dissection of individual sulfation sites identified 2-O, 3-O-de-sulfated heparin (ODSH) as a differentiating agent that suppressed orthotopic xenograft growth while avoiding anticoagulation. These studies uncover the critical components of the differentiating stroma secretome in neuroblastoma and the central mechanism by which they act. We translate these biologic insights into novel prognostic and therapeutic biomarkers, as well as therapeutic differentiation strategies for clinical development. More generally, our work demonstrates that tumor stroma biology can inform design of targeted molecular therapeutics. Citation Format: Erik H. Knelson, Angela L. Gaviglio, Jasmine C. Nee, Mark D. Starr, Andrew B. Nixon, Stephen G. Marcus, Gerard C. Blobe. Stroma biology identifies heparins as differentiating agents in neuroblastoma. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2674. doi:10.1158/1538-7445.AM2014-2674
Abstract 3481: A Novel Mechanism Contributing to End Organ Damage in Endotoxic Shock: Role of TRPV1
Authors: Youping Wang, Donna H Wang
Journal: Circulation (2008)

We previously showed that transient receptor potential vanilloid type 1 (TRPV1) channels play a vital role in the survival of animals during endotoxic shock. To examine effects of TRPV1 on systemic hemodynamics, inflammatory responses, and renal and liver function/damage during endotoxic shock, lipopolysaccharide (LPS, 10 mg/kg) was injected i.p. into wild type (WT) and TRPV1-null mutant (TRPV1 −/− ) mice. Mean arterial pressure (MAP, mmHg) measured with radiotelemetry in conscious mice were severely decreased 11 hours after LPS injection in WT and TRPV1 −/− mice, with no difference between the two strains (76±6 vs. 71±4, p&gt;0.05). The serum levels of alanine aminotransferase (ALT, indicator of liver dysfunction, IU/L) or creatinine (indicator of renal dysfunction, mg/dL) were increased 6 and 24 h after LPS injection in WT and TRPV1 −/− mice, with a greater magnitude in the latter strain (ALT, 79±11 vs. 105±16; creatinine, 0.7±0.09 vs. 1.2±0.08, p&lt;0.05). Serum cytokine (pg/ml) and chemokine (ng/ml) levels were higher 6 h after LPS injection in TRPV1 −/− compared to WT mice (TNF-α, 718±43 vs. 428±44; IL-1β, 736±39 vs. 420±46; KC, 100±8 vs. 60±7; MIP-2, 2.2±0.4 vs. 1.3±0.3, p&lt;0.05). LPS-induced neutrophil infiltration (cells/mm 2 ) was greater in the kidney and liver in TRPV1 −/− than in WT mice (kidney, 93±6 vs. 38±4; liver, 247±13 vs. 156±12, p&lt;0.05). VCAM-1 or ICAM-1 expression was increased 6 and 24 h after LPS injection in the kidney and liver in WT and TRPV1 −/− mice, with a greater magnitude in the latter strain (p&lt;0.05). Renal glomerular hypercellularity and hepatocellular injury were apparent 24 h after LPS injection in WT and TRPV1 −/− mice, with greater cellular density or injury in the latter strain. These data demonstrate that deletion of TRPV1 had no effect on LPS-induced fall in MAP, but accelerated organ damage during endotoxic shock. Exaggerated organ damage was accompanied by increased inflammatory responses as evident by enhanced neutrophil infiltration, proinflammatory cytokine production, and adhesion molecule expression. These data indicate that activation of TRPV1 attenuates organ damage possibly via its anti-inflammatory action without affecting systemic hemodynamics during endotoxic shock.
Prognostic Impact Of Comorbidity In Multiple Myeloma
Authors: Rafael Ríos Tamayo, Joaquín Martínez López, Manuel Jurado, María Esther Clavero Sánchez, Fátima López Jiménez, María Liz Paciello Coronel, José Ramón García Fernández, Youssef Moatassim de la Torre, Francisco López Berenguel, Rosario Leyva Ferrer, Juan Sáinz Pérez, Elena Molina Portillo, María José Sánchez Pérez, José María de Pablos Gallego, Pilar López Garrido, José Juan Jiménez Moleón, Amaia López de Lacalle, Juan José Lahuerta Palacios
Journal: Blood (2013)
Structural and functional impairment of endocytic pathways by retinitis pigmentosa mutant rhodopsin-arrestin complexes
Authors: Jen-Zen Chuang, Carrie Vega, Wenjin Jun, Ching-Hwa Sung
Journal: Journal of Clinical Investigation (2004)

Retinitis pigmentosa (RP) is a clinically and genetically heterogeneous degenerative eye disease. Mutations at Arg135 of rhodopsin are associated with a severe form of autosomal dominant RP. This report presents evidence that Arg135 mutant rhodopsins (e.g., R135L, R135G, and R135W) are hyperphosphorylated and bind with high affinity to visual arrestin. Mutant rhodopsin recruits the cytosolic arrestin to the plasma membrane, and the rhodopsin-arrestin complex is internalized into the endocytic pathway. Furthermore, the rhodopsin-arrestin complexes alter the morphology of endosomal compartments and severely damage receptor-mediated endocytic functions. The biochemical and cellular defects of Arg135 mutant rhodopsins are distinct from those previously described for class I and class II RP mutations, and, hence, we propose that they be named class III. Impaired endocytic activity may underlie the pathogenesis of RP caused by class III rhodopsin mutations.
A memorable patient: Not wanted anywhere
Authors: M. Roy
Journal: BMJ (2000)

active verb "prevents" are often not an accurate description of the findings of clinical studies.A Medline search on 12 active verbs in titles showed that informative titles are becoming more common.There was a post hoc relation between occurrence of searched active verbs and year: the percentage is increasing with the square of the year, such that 4.5% (95%CI: 3.8%-5.8%) of all titles of clinical reports published in the year 2010 will contain one.There may be arguments for reviews and editorials carrying informative titles, but they are too often wrong to have any place in the reporting of research.Journals should ask for indicative titles, or alter investigators' informative titles during sub-editing.
Terbutaline Depot Tablets in Asthma
Authors: Ebbe Taudorf, Allan Bundgaard, Per Olof Fagerstrom, Eva Weeke, Bent Weeke
Journal: Allergy (1981)

A sustained release preparation of terbutaline sulphate has been formulated (Bricanyl depot tablets) in order to extend the duration and accordingly change the dosage regimen to twice a day. This presentation gives a summary of a clinical trial performed in order to study effect and side effects of terbutaline depot tablets 7.5 mg twice a day compared to terbutaline tablets 5 mg three times a day. Patients suffering from perennial asthma and with daily requirement of asthma medicine were accepted for the study. The trial was a double-blind cross-over, double dummy and randomized. The tablets were given in two consecutive periods of 7 day's duration each. The effect of terbutaline depot tablets was equal to the effect of the ordinary terbutaline tablets. The indication for using depot tablets in the basic treatment of bronchial asthma is a better patient compliance due to medication twice a day. Furthermore in patients with unstable bronchial asthma and in patients with morning dips in PEF the more stable plasma concentration may perhaps keep the patients in a more steady state.
Shh pathway activity is maintained in direct allografts of medulloblastoma cells whereas it is irreversibly lost in cell culture: Implications for preclinical studies of smoothened antagonists
Authors: Ken Sasai, Justyna T. Romer, Tom Curran
Journal: Cancer Research (2006)

4846   Medulloblastoma, the most common malignant pediatric brain tumor, accounts for approximately 20% of malignant pediatric brain tumors. Current treatment, which involves surgical resection, chemotherapy and radiation of the craniospinal axis, is often associated with major long-term side effects, therefore, there is a great need for new nontoxic therapies. Gene expression profiling indicates that the Sonic Hedgehog (Shh) pathway is active in approximately 30% of human medulloblastomas and it has been suggested that it could provide a useful therapeutic target. Previously, we demonstrated that spontaneous medulloblastomas in Ptc1()p53(-/-) mice could be eradicated by treatment with a small molecule inhibitor (HhAntag) of Smoothened (Smo) (Romer et al., 2004 Cancer Cell). However, spontaneous genetic models recapitulate only a specific disease subset and they do not encompass the full disease spectrum of the corresponding human cancer. In addition, genetic models are often not suited to high throughput studies of potential new treatments. Therefore, it is important to complement studies on genetic models with alternative approaches involving cancer cells propagated in vitro and in vivo. We compared the responses of mouse medulloblastoma cells propagated in flank allografts, either directly or after culture in vitro, to HhAntag. We found that Shh pathway activity was suppressed in medulloblastoma cells cultured in vitro and it was not restored when these cells were transplanted into flank allografts. The growth of these transplanted tumor cells was not inhibited by treatment of mice with doses of HhAntag that completely suppressed Smo activity. In contrast, tumor cells transplanted directly into the flank maintained Smo activity and they were completely eliminated by treatment with HhAntag. These findings indicate that propagation of tumor cells in culture inhibits Smo activity in a way that cannot be reversed by transplantation in vivo and that the growth environment plays a significant role in regulating the Shh pathway. Affymetrix cDNA microarray analysis indicated that the gene expression profiles of tumor cell cultures and corresponding allograft were strikingly different from those of the original medulloblastomas. In contrast, the directly-transplanted allografts differed only modestly from the original tumors.