tbuckley/cabot-search huggingface dataset:
Studies on the Susceptibility of C57BL/6 Mice to Rauscher Virus. I. Properties of Rauscher Virus-Induced C57BL/6 Lymphomas&lt;xref ref-type="fn" rid="FN2"&gt;2&lt;/xref&gt;
Authors: Akinori Ishimoto, Michiyuki Maeda
Journal: JNCI Journal of the National Cancer Institute (1970)

Rauscher virus was inoculated info 113 newborn mice of the C57BL/6 strain. Lymphoma developed in 46 mice. Twenty-eight lymphomas were transplantable and ten were not. All originally induced lymphomas, except one designated RD-2, produced virus which induced early hepatosplenomegaly in SMA and BALB/c mice and lymphoma in C57BL/6 mice. In contrast, cell-free materials derived from RD-2 lymphoma initiated only lymphomas after relatively long incubations in C57BL/6, SMA, and BALB/c mice. This “RD-2 virus” was transmissible by cell-free materials of lymphoma without any alteration in its leukemogenic potency and properties. Friend-Moloney-Rauscher (FMR) antigen was found in all lymphomas, including those induced with RD-2 virus. Viral activity inducing erythroblasfosis associated with the long-transplanted lymphomas seemed to decrease remarkably with successive transplant passages. Weanling SMA mice inoculated with 10−3 or 10−4 diluted cell-free materials from originally induced tumors developed early splenomegaly with eryfhroblastosis. However, weanling mice inoculated with the undiluted materials from long-transplanted lymphomas did not develop any leukemias. Only newborn mice inoculated with the undiluted materials from long-transplanted lymphomas developed early splenomegaly. The limiting-dilution method was applied to Rauscher virus, but highly diluted virus caused no leukemias of any type.
The Tranylcypromine Isomers : A Controlled Clinical Trial
Authors: JAVIER I. ESCOBAR, BURTRUM C. SCHIELE, ROBERT ZIMMERMANN
Journal: American Journal of Psychiatry (1974)

The plus (+ ) and minus (–) isomers of tranylcypromine (Parnate) were tested under double-blind conditions on 11 depressed patients. The (—) isomer was the more effective and produced fewer side effects. Because the (–) isomer has been shown to be a stronger blocker of the reuptake mechanism for brain amines and a weaker inhibitor of monoamine oxidase than the (+) isomer, the results obtained here are of particular interest.
Community Psychiatry in Malaysia
Authors: J. D. KINZIE, J. I. TEOH, E. S. TAN
Journal: American Journal of Psychiatry (1974)

This report traces the first year's experience in community psychiatry in a developing country. Malaysia, where a group of psychiatrists decided to offer their services as consultants to community caregivers, including doctors, teachers, police, the clergy, and native healers. The psychiatrists had mixed success, depending largely on whether or not the consultees shared their attitudes and values. The authors conclude that the entire sociocultural setting of a developing country must be examined closely as to its underlying concepts and value systems before one can assess how open the culture is to mental health consultation. The consultants must be aware of the implicit and explicit value systems underlying the psychiatric consultation, as these may be different from those of the consultees and clients.
Temporally and Spatially Coordinated Expression of Cell Cycle Regulatory Factors After Angioplasty
Authors: Geeyeon Laura Wei, Kevin Krasinski, Marianne Kearney, Jeffrey M. Isner, Kenneth Walsh, Vicente Andres
Journal: Circulation Research (1997)

Intimal hyperplasia following angioplasty results in part from the migration and proliferation of vascular smooth muscle cells (VSMCs). However, the cell cycle regulatory networks underlying injury-induced VSMC proliferation are largely unknown. In the present study, we examined the kinetics of expression and activity of cell cycle regulatory factors after angioplasty in rat and human arteries. Cell lysates were prepared from uninjured rat carotid arteries and at different time points after balloon denudation. Marked induction of the proliferating cell nuclear antigen (PCNA), the G1/S cyclin-dependent kinase (cdk2), and its regulatory subunits (cyclin E and cyclin A) occurred between 1 and 2 days after angioplasty, was sustained up to 10 days after injury, and then declined. Induction of these factors correlated with increased cdk2-, cyclin E-, and cyclin A-dependent kinase activity, indicating the assembly of functional cdk2/cyclin E and cdk2/cyclin A holoenzymes in the injured arterial wall. Immunohistochemical analysis revealed early expression of cdk2, cyclin E, and PCNA within the media of injured carotid arteries. At later time points, expression of these markers declined to basal levels in the media but was detected within the intimal lesion. Thus, VSMC proliferation after angioplasty in the rat carotid artery is associated with a temporally and spatially coordinated expression of cdk2, cyclins E and A, and PCNA. Analysis of human arteries also revealed expression of these factors in VSMCs within restenotic lesions. Thus, cdk2 and its regulatory cyclins may be suitable targets to limit human restenosis.
Nanomechanics of Cells and Biomaterials Studied by Atomic Force Microscopy
Authors: Jason I. Kilpatrick, Irène Revenko, Brian J. Rodriguez
Journal: Advanced Healthcare Materials (2015)

The behavior and mechanical properties of cells are strongly dependent on the biochemical and biomechanical properties of their microenvironment. Thus, understanding the mechanical properties of cells, extracellular matrices, and biomaterials is key to understanding cell function and to develop new materials with tailored mechanical properties for tissue engineering and regenerative medicine applications. Atomic force microscopy (AFM) has emerged as an indispensable technique for measuring the mechanical properties of biomaterials and cells with high spatial resolution and force sensitivity within physiologically relevant environments and timescales in the kPa to GPa elastic modulus range. The growing interest in this field of bionanomechanics has been accompanied by an expanding array of models to describe the complexity of indentation of hierarchical biological samples. Furthermore, the integration of AFM with optical microscopy techniques has further opened the door to a wide range of mechanotransduction studies. In recent years, new multidimensional and multiharmonic AFM approaches for mapping mechanical properties have been developed, which allow the rapid determination of, for example, cell elasticity. This Progress Report provides an introduction and practical guide to making AFM‐based nanomechanical measurements of cells and surfaces for tissue engineering applications.
Difficult to wean patients after cardiac and thoracic surgery: outcome results of a regional weaning center
Authors: Guido Vagheggini, Eugenia Panait, Adriano Di Paco, Stefano Mazzoleni, Fabio Guarracino, Anna Zito, Fabrizio Mezzasalma, Michela Digiorgio, Dewi Makhabah, Nicolino Ambrosino
Journal: European Respiratory Journal (2013)

To evaluate the outcomes of a cohort of prolonged weaning patients after thoracic and cardiac surgery, treated in a specialized weaning centre, we retrospectively analyzed characteristics and outcome of patients consecutively admitted to our regional Weaning Center (WC) since December 2007 to August 2012 (56 months). Among overall 585 patients (337 males, age 70.7±11.7yrs, range 18.8-93.9yrs), 35 patients (17 males; age 73.0±10.1yrs, range 43.4-86.2yrs) after isolated (valve replacement or myocardial revascularization) or combined (both) surgery were also admitted. Twelve patients had undergone emergency surgery; ICU Length of stay had been 56.5±32.0 days. Apache II score at WC admission was 13.5±5.8; SAPS score 35.9±8.6, Charlson score 6.8±2.7, PaO2/FiO2 242.2±88.1. The weaning success rate was 65.7% (23 patients), 20 of them being decannulated. Two patients were transferred to ICU for complications, and 6 patients (17.1%) needed prolonged invasive mechanical ventilation at discharge from WC. The WC length of stay was 26.8±11.3 days, the overall mortality rate was 11.4% (4/35). Lower weaning rate (7/16 vs. 16/19; p=0.03) and a tendence to higher mortality (3/16 vs. 1/19; p= n.s.) were observed in combined surgery group. Survival at 6 months is shown in Figure 1.  These results suggest the usefulness of dedicated weaning centers for difficult-to wean patients undergone cardiac surgery.
Phase I trial of dose-intense liposome-encapsulated doxorubicin in patients with advanced sarcoma.
Authors: E S Casper, G K Schwartz, A Sugarman, D Leung, M F Brennan
Journal: Journal of Clinical Oncology (1997)

PURPOSE To define the maximum-tolerated dose (MTD) of liposome-encapsulated doxorubicin (LED) when used every 2 weeks with granulocyte colony-stimulating factor (G-CSF) in patients with advanced soft tissue sarcoma. PATIENTS AND METHODS Doxorubicin encapsulated in egg phosphatidylcholine/cholesterol liposomes was given to patients with sarcoma in a disease-specific phase I trial. The initial dose was 75 mg/m2 with G-CSF 5 micrograms/kg. The MTD was defined as the highest dose that could be given every 2 weeks. RESULTS Twenty-nine patients participated in this study. Major toxicities included myelosuppression, nausea and vomiting, fatigue, and mucositis. Eight patients were hospitalized for nadir fever. No cardiotoxicity was seen. The MTD was LED 105 mg/m2 with G-CSF 5 micrograms/kg. LED 120 mg/m2 resulted in tolerable, albeit prominent, acute toxicity, but did not permit recycling of therapy on day 15. Among 26 patients with soft tissue sarcoma, 23 had measurable disease, of whom three achieved a partial response (13%; 95% confidence interval, 2% to 34%). CONCLUSION LED can be administered every 2 weeks at a dose of 105 mg/m2 with G-CSF support, which provides a dose-intensity of 52.5 mg/m2/wk. To exceed this intensity, the dose of LED that would have to be administered every 3 weeks would be greater than 157.5 mg/m2. A formal phase II trial is needed to estimate better the true response rate.
THE EARLY DIAGNOSIS OF EPIDEMIC HEMORRHAGIC FEVER—EXPERIENCES IN THE FORWARD ECHELONS OF THE MEDICAL SERVICE
Authors: Edward F. Counts, Raymond Seltser
Journal: Annals of Internal Medicine (1953)

Article1 January 1953THE EARLY DIAGNOSIS OF EPIDEMIC HEMORRHAGIC FEVER—EXPERIENCES IN THE FORWARD ECHELONS OF THE MEDICAL SERVICEEDWARD F. COUNTS, RAYMOND SELTSEREDWARD F. COUNTSSearch for more papers by this author, RAYMOND SELTSERSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-38-1-67 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptAt Division level it is extremely important that the diagnosis of epidemic hemorrhagic fever be suspected as early as possible, so that evacuation may be instituted to take the patient to an installation better equipped to handle the numerous problems arising in the care of such patients.In order to evaluate criteria which may be useful in considering the diagnosis, a study was made of 50 consecutive patients evacuated from a divisional sector with the diagnosis of epidemic hemorrhagic fever. The data were submitted to the Division Surgeon's Office in the form of telegraphic reports in which were listed the... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: EDWARD F. COUNTS; RAYMOND SELTSER*Received for publication July 25, 1952. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byRadiologic manifestations of epidemic haemorrhagic fever with renal syndromeHereditäre und endemische Nephropathien vorwiegend interstitieller LokalisationArbor-Viren (durch Arthropoden übertragbare Viren)THE FORTY-NINTH STARRENAL SEQUELÆ OF EPIDEMIC HÆMORRHAGIC FEVERDas epidemische h�morrhagische FieberThe sequelae of epidemic hemorrhagic feverAnalysis of sequential physiologic derangements in epidemic hemorrhagic fever 1 January 1953Volume 38, Issue 1 Page: 67-72 Keywords Hemorrhagic fevers Medical services Patients ePublished: 1 December 2008 Issue Published: 1 January 1953 PDF downloadLoading ...
Fibronectin mRNA in the developing glomerular crescent in rabbit antiglomerular basement membrane disease.
Authors: S P Sady, M Goyal, P E Thomas, B L Wharram, R C Wiggins
Journal: Journal of the American Society of Nephrology (1995)

Fibronectin is a multifunctional matrix protein important in wound healing that is markedly increased in glomerular crescents. A previous report established two phases of fibronectin metabolism in crescent formation in an anti-glomerular basement membrane model of crescentic nephritis in the rabbit. Phase I was associated with increased glomerular fibronectin content from plasma. Phase II was associated with increased fibronectin mRNA in glomeruli. To examine the hypothesis that fibronectin is synthesized in the developing crescent, rabbit fibronectin cDNA was cloned, sense and antisense riboprobes were prepared and their specificity under the conditions to be used was validated and in situ hybridization studies were performed in the model. The results showed that the cells in the developing glomerular crescent express an intense fibronectin mRNA signal at Day 7 and that this signal persisted in cells of the crescent at Day 14. This result shows that fibronectin synthesis does indeed take place in cells of the developing crescent in this model and supports the hypothesis that fibronectin may be an important agent regulating crescent formation and fibrosis.
Abstract 207: Unc-51-like Kinase 1 (ULK1) Plays A Crucial Role In Mitophagy In Cardiomyocytes
Authors: Toshiro Saito, Junichi Sadoshima
Journal: Circulation Research (2014)

The mitochondrion is an essential organelle that supplies ATP in cardiomyocytes (CMs). However, damaged mitochondria are harmful via the production of reactive oxygen species and induction of apoptosis in pathological conditions. Therefore, quality of mitochondria should be controlled tightly through various mitochondrial quality control mechanisms. Mitochondrial autophagy (mitophagy) is considered an integral part of this mechanism, and recent investigations uncovered the role of PINK1 and Parkin in mitophagy. However, these observations were made under artificial conditions, such as over-expression of Parkin or treatment with CCCP, and thus the precise mechanism has not been fully elucidated in more pathophysiologically relevant conditions. Recent evidence suggests that mitophagy can take place independently of ATG7, a molecule essential for the conventional form of autophagy, and that this form of autophagy is ULK1-dependent. We investigated the role of ULK1 and ATG7 in mediating mitophagy using mitochondria-targeted Keima (Mito-Keima) in cultured rat neonatal CMs. Keima has a bimodal excitation spectrum peaking at 440 and 560 nm, corresponding to the neutral and acidic pH, respectively. In CMs transfected with Mito-Keima, the fluorescent dots with a high 560nm/440nm ratio represent the mitochondria incorporated into autolysosomes which indicate mitophagy. Here we report that ULK1 plays a more predominant role in glucose deprivation (GD) -induced mitophagy than ATG7. Control CMs exhibited 8.7±1.0 % of the area of high-ratio dots per cells after GD. Knockdown of ULK1 significantly reduced the area to 2.3±0.9 % in CMs after GD (p&lt;0.01, vs sh-Control). The reduction was significantly greater in CMs with knockdown of ULK1 than that of ATG7 (7.0±1.6 %, p&lt;0.05, sh-ULK1 vs sh-ATG7). In addition, knockdown of Beclin1 and Drp1 also significantly decreased the area of high-ratio dots (about 1.0 % and 0.5 %, respectively). Overexpression of ULK1 was sufficient to induce mitophagy without starvation, whereas that of ATG7 was not. These results suggest that ULK1, Beclin1 and Drp1 play an essential role in mediating GD-induced mitophagy in CMs.
Clinical impact of the complications related to lung transplantation
Authors: Ana Verónica Cardoso, Helder Novais e Bastos, Carla Damas
Journal: European Respiratory Journal (2014)

Introduction: For selected patients, lung transplantation (LT) prolongs survival and improves quality of life. However, it is also associated with complications with high morbidity.

Objective: To evaluate the impact of the complications associated to LT in quality of life and functional status of transplanted patients.

Methods: Analysis of the complications and its correlation with quality of life, physical limitation and anxiety/depression, evaluated, respectively, by applying the Medical Outcomes Study Short Form-36 (SF-36), the scale London Chest Activity of Daily Living (LCADL) and the Hospital Anxiety and Depression Scale (HADS).

Results: 30 transplanted patients were included. The most frequent complications were: osteoporosis (46.7%), CMV infection (40%), renal dysfunction (33.3%) and dyslipidemia (33.3%). There was a negative correlation between complications number and quality of life in the areas of bodily pain (r=-0.36, p=0.04), vitality (r=-0.37, p=0.04), emotional role functioning (r =-0.37, p=0.04) and mental health (r=-0.39, p=0.03). No significant association was established with the remaining four domains of the SF-36. We found a positive correlation between complications and physical limitations in the field of self-care of LCADL (r=0.45, p=0.01). This association was not observed with other areas neither with the total score of LCADL. Anxiety and depression were not correlated with complications.

Conclusion: The complications associated to LT contribute to decreased quality of life and increased physical limitation of patients. However, the presence of low to moderate associations with only some fields of the SF-36 and LCADL, suggests that there are other factors influencing these variables.
Safety and efficacy of TACE and gamma knife on hepatocellular carcinoma with portal vein invasion
Authors: Xiao-Jie Lu, Jing Dong, Li-Juan Ji, Jin-Hong Luo, Huang-Ming Cao, Li-Xin Xiao, Jun Zhou, Chang-Quan Ling
Journal: Gut (2015)

We read with interest the article by Bruix et al 1 in which Sorafenib was regarded as the standard treatment for hepatocellular carcinoma (HCC) patients with portal vein tumour thrombosis (PVTT). However, Sorafenib can only confer 2–3 months of overall survival (OS) benefit2 ,3 and is inapplicable to many patients due to extensive side effects and high price. Zhong et al 4 proposed that liver resection might provide survival benefit to patients with HCC-PVTT with adequate liver function. However, this proposal was based on literature survey, lacked definite patient inclusion criteria and failed to provide information on patients’ baseline characteristics. Therefore, explorations of alternative therapeutics for patients with HCC-PVTT are still needed.

Although not recommended by mainstream guidelines5 ,6 for patients with HCC-PVTT, transarterial chemoembolisation (TACE) has long been practiced in the clinic in selected patients with HCC-PVTT.7 ,8 Gamma knife surgery (GKS) has shown favourable effects in treating brain …
Phase II Trial of the Anti-G<sub>D2</sub>Monoclonal Antibody 3F8 and Granulocyte-Macrophage Colony-Stimulating Factor for Neuroblastoma
Authors: Brian H. Kushner, Kim Kramer, Nai-Kong V. Cheung
Journal: Journal of Clinical Oncology (2001)

To describe oncolytic effects of treatment with anti-G(D2) monoclonal antibody 3F8 plus granulocyte-macrophage colony-stimulating factor (GM-CSF) in patients with neuroblastoma (NB).Patients were eligible for 3F8/GM-CSF if intensive therapy had not eradicated potentially lethal NB. One cycle consisted of GM-CSF (subcutaneous bolus) on days 1 through 5, 11, and 12, and GM-CSF (2-hour intravenous [IV] infusion) followed after a 1-hour interval by 3F8 (1.5-hour IV infusion) on days 6 through 10 and 13 through 17. GM-CSF was dosed at 250 microg/m(2)/d on days 1 through 7 and at 500 microg/m(2)/d on days 8 through 17. 3F8 was dosed at 10 mg/m(2)/d (100 mg/m(2)/cycle). 3F8 was given with an opiate and an antihistamine. Patients without progressive disease (PD) or elevated human antimouse antibody titers could be treated again beginning 3 weeks after completion of a cycle.Among 19 patients treated for NB resistant to induction therapy, 12 of 15 had complete remission (CR) of bone marrow (BM) disease, and three others who had less than partial responses achieved prolonged progression-free survival (one remains on study at 21+ months, two had PD at 12 and 17 months). Among patients treated for recurrent NB resistant to retrieval therapy, five of 10 had CR in BM. The 15 patients treated for PD fared poorly, although two had scintigraphic findings suggestive of a short-term response. Side effects were limited to readily manageable pain and, less commonly, rash of short duration; hence, patients were treated as outpatients.3F8/GM-CSF is well tolerated and shows promise for treatment of minimal residual NB in BM.
Clinical effectiveness of anti-IgE therapy in severe asthma with fungus sensitization
Authors: Shu-Min Lin, Chun-Hwa Wang, Han-Pin Kuo
Journal: European Respiratory Journal (2014)

Background:  Patients with severe asthma with fungus sensitization (SAFS) are immunologically sensitive to fungi. It is not known whether SAFS is responsive to anti-IgE treatment.  Methods:  A prospective open label study was conducted on 99 patients with allergic severe asthma. Omalizumab was administered to all the asthmatics with serum IgE >70 IU/ml. Clinical effectiveness of omalizumab was assessed every 3 months. For patients with partial control of asthma, therapeutic trials of anti-fungal therapy were added to omalizumab. For patients with complicated rhinosinusitis, surgical intervention was applied.  Results:  39.4% (39/99) of our allergic severe asthmatics are fungus sensitization. Among them, 33 SAFS patients (84.6%) responded to omalizumab alone in terms of improvement of asthma control, reduction of ER visit, hospitalization, dosage of steroids. The responsive rate was similar in non-SAFS asthmatics (86.7%, n=60, P>0.1). 61.5% (n=24) and 23.1% (n=9) of SAFS patients achieved well control and partial control of asthma, respectively after 3 months of treatment. Similarly, 64.3% (n=38) and 23.3% (n=14) of non-SAFS patients achieved well control and partial control of asthma, respectively. 66.7% (10/15) of not well-controlled SAFS patients attained well control after 3 months of additional itraconazole, compared to only 9.1% (2/22, P In conclusion,  omalizumab is effective in treatment of SAFS. Additional anti-fungal therapy or rhinosinus surgery is required for patients who are not well controlled with omalizumab.