tbuckley/cabot-search huggingface dataset:
(-)-gossypol-enriched cottonseed oil induces apoptosis in multi-drug resistant human breast cancer cells.
Authors: Weiping Ye, Li-Shu Wang, Yi-Wen Huang, Weicheng Huang, Yan Yan, Michael Down, Peter Wan, Young Lin
Journal: Cancer Research (2007)

AACR Annual Meeting-- Apr 14-18, 2007; Los Angeles, CA

3221 

Chemotherapy remains an important option in breast cancer treatment. However, emergence of resistance to chemotherapeutic agents is a concern for breast cancer therapy. Increasing evidence indicates that resistance may be associated with defects, or dysregulation of, different steps of the apoptotic pathway. Recently research showed that gossypol (GP) possesses anti-neoplastic activity against a variety of malignant cell types in vitro and in vivo . We investigate the effect of (-)-gossypol-enriched cottonseed oil [(-)-GPCSO], which contains 65% of (-)-GP and 35% of (+)-GP on induction of apoptosis in a multi-drug resistant human breast cancer cell line, MCF-7 Adr and primary cultured human breast cancer epithelial cells (PCHBCECs) using non-radioactive cell proliferation assay, quick apoptotic DNA ladder detection, RT-PCR and Western Blotting analysis after two types of cells were treated with 0.025, 0.05 and 0.1% of (-)-GPCSO for 24 hours. Our results demonstrated that (-)-GPCSO inhibited proliferation and induced DNA fragmentation in a dose-dependent manner in both cell types. 0.05 and 0.1% of (-)-GPCSO significantly decreased Bcl-2 mRNA and protein expression in MCF-7 Adr cells to 14 and 32%, and 65 and 82% respectively. In PCHBCECs, the same concentrations of (-)-GPCSO treatment significantly reduced Bcl-2 mRNA and protein expression to 60 and 93% and 71 and 76%, respectively. Evaluation of the effect of (-)-GPCSO on Bax mRNA and protein expression was also performed. Compared to controls, treatment of 0.025, 0.05 and 0.1% of (-)-GPCSO significantly increased Bax mRNA expression to 40, 47, 116% in MCF-7 Adr cells, and 100, 123, 423% in PCHBCECs. The same concentrations of (-)-GPCSO significantly up-regulated Bax protein expression to 27, 50 and 62% in MCF-7 Adr cells. Our results demonstrated that (-)-GPCSO possesses potent anti-mitogenic effects on human breast cancer cells, its ability in suppressing breast cancer cell proliferation may be mediated through down-regulation of Bcl-2 and up-regulation of Bax expression in both mRNA and protein levels. Our experimental data suggest that natural food components, (-)-GPCSO might serve as low costs and effective chemopreventive ingredients for healthy consumers and anti-multidrug resistance food components for breast cancer patients (Supported by NIH Grants CA94718 & CA95915, and Cotton Incorporated Project 06-864).
The UGT2B28 Sex-steroid Inactivation Pathway Is a Regulator of Steroidogenesis and Modifies the Risk of Prostate Cancer Progression
Authors: Anaïs Belledant, Hélène Hovington, Luciana Garcia, Patrick Caron, Hervé Brisson, Lyne Villeneuve, David Simonyan, Bernard Têtu, Yves Fradet, Louis Lacombe, Chantal Guillemette, Eric Lévesque
Journal: European Urology (2015)
A temperature-sensitive disorder in basal transcription and DNA repair in humans
Authors: Wim Vermeulen, Suzanne Rademakers, Nicolaas G.J. Jaspers, Esther Appeldoorn, Anja Raams, Binie Klein, Wim J. Kleijer, Lars Kjærsgård Hansen, Jan H.J. Hoeijmakers
Journal: Nature Genetics (2001)
Abstract P033: Alcohol and Incident Heart Failure among the Cohort of Swedish Men
Authors: Kirsten S Dorans, Elizabeth Mostofsky, Emily B Levitan, Niclas Håkansson, Alicja Wolk, Murray A Mittleman
Journal: Circulation (2014)

Background: The relationship between alcohol intake and incident heart failure (HF) is complex. Compared with no alcohol consumption, heavy alcohol intake is associated with higher HF risk whereas light or moderate consumption may be associated with lower risk. Methods: We analyzed 34,581 men 45-79 years old with no history of HF, diabetes mellitus or myocardial infarction (MI) who were participants in the population-based Cohort of Swedish Men study. We excluded former drinkers. At baseline, participants reported how frequently they consumed specific alcoholic beverages in the past year and other characteristics. HF was defined as hospitalization for or death from HF (primary diagnosis), ascertained by Swedish inpatient and cause-of-death records from January 1, 1998 to December 31, 2011. Due to missing values for two covariates, we used Markov chain Monte Carlo multiple imputation to simulate 5 complete datasets. We used Cox proportional hazards models to estimate rate ratios and 95% confidence intervals, adjusting for age, total energy intake, education, body mass index, physical activity, a dietary component score, cigarette smoking, marital status, family history of MI before age 60, history of hypertension and history of high cholesterol. Results: At baseline, mean age was 58.6 years. There were 1592 incident cases of HF over the follow-up period. Compared with never drinkers, the multivariable-adjusted rate of HF was 19% lower among men who drank &lt;1 drink per week (rate ratio: 0.81, 95% confidence interval: 0.64, 1.04%). The multivariable-adjusted HF rate was similar among men who drank &lt;1 drink per week and men who drank ≥1 drink per week. Conclusions: Light-to-moderate drinking is associated with lower rate of HF compared with never drinking, but the association was not statistically significant.
Abstract P098: Maternal Insulin/Glycemic Regulation, Leptin, and Adiposity Levels are Associated with Newborn Adiposity
Authors: Julie Patenaude, Catherine Allard, Marilyn Lacroix, Laeticia Guillemette, Marie-Claude Battista, Myriam Doyon, Julie Moreau, Patrice Perron, Marie-France Hivert
Journal: Circulation (2014)

Introduction: Leptin is a hormone secreted by adipocytes that circulates in proportion to body fat. Placenta also produces leptin suggesting a specific role during pregnancy, maybe contributing to pregnancy-induced insulin resistance. Objective: To determine the associations between maternal levels of leptin, glucose and insulin and weight/adiposity measures in newborns. Methods: A population-based cohort of pregnant women in the region of Sherbrooke, Canada was recruited in early pregnancy (n=1040 at 1 st trimester). Maternal anthropometry was measured and blood samples were collected at the 1 st and 2 nd trimesters to measure glucose (hexokinase), leptin and insulin (ELISA Luminex, Millipore Canada). Birth weights were recorded from the medical records in 783 newborns (full term &gt;36 weeks). We measured skinfolds in 199 newborns within 72h after birth, using a standardized caliper. We measured triceps, biceps, subscapular and supra-iliac skinfolds and the sum of skinfolds was used for analysis representing overall neonatal adiposity. Correlations and linear regression analyses were performed to evaluate the associations between maternal metabolic characteristics and neonatal weight/adiposity measures. Results: Birth weight was associated with maternal body mass index (BMI; r=0.18 and r=0.20 at 1st and 2 nd trimesters respectively; both P &lt;0.001), percent body fat (%bf; r=0.25; P &lt;0.001), weight gain over pregnancy (r=0.14; P &lt;0.001), and fasting leptin (r=0.15, P &lt;0.001). Surprisingly, crude birth weight was not associated with maternal glycemic or insulin levels at 1 st or 2 nd trimesters (all P &gt;0.05). In contrast, sum of skinfolds was associated with maternal fasting glucose (r=0.17, P =0.01) and fasting insulin (r=0.24, P =0.001), in addition to fasting leptin (r=0.18, P =0.01) at 2 nd trimester. Sum of skinfolds was also associated with maternal BMI (r=0.18, P =0.008 at 1 st and r=0.21, P =0.003 at 2 nd trimester) and %bf (r=0.19; P =0.005). After adjustments for maternal BMI or %bf, sum of skinfolds remained significantly associated with maternal fasting insulin (all P &lt;0.05), but not with maternal leptin levels ( P =0.16 to 0.35) Conclusion: These results suggest that maternal insulin/glycemic regulation has a greater impact on neonatal adiposity than overall crude birth weight. Birth weight and neonatal adiposity are both associated with maternal leptin levels, likely representing maternal own adiposity levels that might influence neonatal outcomes directly or indirectly.
263. Antigen Epitope-Expressing Cytokines for DNA Immunization
Authors: Xianghui He, Tom C. Tsang, Tong Zhang, Phoebe Luo, David T. Harris
Journal: Molecular Therapy (2004)

Strategies to enhance the efficacy of DNA vaccination against malignancy remain to be established. In this study, plasmids expressing tumor antigen incorporated into the signal peptide of human IL-2 were tested as DNA vaccine in murine model system. Results showed that antigen-specific CTL responses were elicited by intramuscular injection of these plasmids. Importantly, compared with minigene vector expressing the same epitope, the antigen epitope-incorporated, IL-2 expression plasmid vaccination was more effective in protecting mice from antigen-expressing tumor challenge. The improved efficacy appears to result from enhanced antigen presentation as well as the immunostimulatory activity of IL-2. This approach may provide new perspectives in designing cytokine-adjuvant DNA vaccines for clinical applications.
Prescription charges: change overdue?
Authors: T Walley
Journal: BMJ (1998)

The NHS prescription charge, currently £5.80, is not related to the cost of the medicine but is a tax for the use of NHS services, intended partly to deter excessive demand for prescriptions. Its deterrent value is currently limited: about half the population are exempt, and only 14% of prescriptions are charged for.1 The charge itself is criticised as a tax on the sick for its rate of rise and high level.2 About 60% of prescriptions cost less than the charge, and many doctors now offer private prescriptions to non-exempt patients, where this will cost less than the charge,3 or advise patients to buy some medicines over the counter.4 Non-exempt patients are less likely to get a prescription dispensed than exempt patients.5 Many anomalies exist: patients with some diseases, such as hypothyroidism, pay no charges even for unrelated medicines, while some groups, such as elderly people, are entirely exempt, irrespective of their financial circumstances.

A recent review concluded that while patient copayments do reduce medicine use, they reduce the use of both desirable and less desirable medicines.6 Experience in Australia suggests that any such detrimental effect is short lived,7 and in the United Kingdom increases in prescription charges lead to only a slight decrease in prescriptions dispensed …
Activation of natural killer T cells by α-galactosylceramide treatment prevents the onset and recurrence of autoimmune Type 1 diabetes
Authors: Shayan Sharif, Guillermo A. Arreaza, Peter Zucker, Qing-Sheng Mi, Jitin Sondhi, Olga V. Naidenko, Mitchell Kronenberg, Yasuhiko Koezuka, Terry L. Delovitch, Jean-Marc Gombert, Maria Leite-de-Moraes, Christine Gouarin, Ren Zhu, Agathe Hameg, Toshinori Nakayama, Masaru Taniguchi, Françoise Lepault, Agnès Lehuen, Jean-François Bach, André Herbelin
Journal: Nature Medicine (2001)
Abstract 16693: Role of PSGL-1 Expressing CD4 T Cells in Plaque Instability
Authors: Keiko Gomita, Kayoko Sato, Michiaki Higashitani, Fumiaki Mori, Motoji Sawabe, Nobuhisa Hagiwara
Journal: Circulation (2010)

Background: Adhesive interactions of leukocytes with the endothelial cells have been known to play a crucial role in atherosclerosis progression and plaque instability. In this study, we examined w...
Multicompartmental Analysis of Cholesterol Metabolism in Man
Authors: Charles C. Schwartz, Mones Berman, Z. Reno Vlahcevic, Leon Swell
Journal: Journal of Clinical Investigation (1982)

The purpose of this study is to delineate the immediate sources and fractional turnover of high density lipoprotein (HDL) esterified cholesterol in man. Various labeled preparations were administered in 11 experiments to six subjects who had either a complete bile fistula (maximally stimulated cholesterol metabolism) or an intact enterohepatic circulation. The administered tracers included [(3)H]mevalonic acid; [(14)C]cholesterol bound to albumin; low density lipoprotein (LDL) free [(3)H] or [(14)C]cholesterol; HDL free [(3)H] or [(14)C]cholesterol; HDL esterified [(3)H]cholesterol; and LDL esterified [(3)H]cholesterol. Blood samples were obtained at frequent intervals for up to 5 d after the administration of tracers. The mass and radioactivity in individual plasma lipoprotein (very low density lipoprotein [VLDL], HDL, and LDL) free and esterified cholesterol were determined. The data were subjected to multicompartmental analysis using the SAAM-27 computer program. The analysis revealed that plasma free cholesterol was not the only immediate source of either a single- or two-compartment HDL ester system. When LDL esters and plasma (HDL) free cholesterol were tested together as sources of one HDL ester compartment, data from all the experiments were readily fit. The fluxes arrived at with the final model indicated that only approximately 20% of the esterified cholesterol in HDL was newly synthesized from plasma (HDL) free cholesterol (2.36 mumol/min); the remaining 80% was from LDL ester (8.92 mumol/min). The presence of a bile fistula had no obvious effect on HDL esterified cholesterol metabolism. The rate of HDL cholesterol ester turnover was 3-12 times/d, indicating that the ester component of the HDL particle is in a very dynamic state.
Studies on the Relationship of Hemadsorption by Normal Murine Spleen and Murine Tumors
Authors: Loren J. Humphrey
Journal: Cancer Research (1967)

The relationship of hemadsorption by methylcholanthrene-induced sarcoma (MC-Sa) and normal murine spleen was studied. Since the hemadsorbing factor of spleen and MC-Sa sections appeared identical, pathologic lesions were created in mice, and these were found to hemadsorb the same species' erythrocytes that are adsorbed by spleen and MC-Sa. In further studies, spleen and MC-Sa sections from mice preimmunized with either bovine or porcine erythrocytes were found to adsorb those species' erythrocytes used for preimmunization. Although human erythrocytes treated by receptor-destroying enzyme of Vibrio comma (RDE) were not adsorbed to spleen and MC-Sa sections, bovine and porcine erythrocytes treated with RDE were adsorbed to spleen and MC-Sa sections obtained from preimmunized mice. Significant differences were also noted between the hemadsorbing factor and the hemagglutination in preimmunized mice. By washing sections of spleen or MC-Sa obtained from preimmunized mice, it was found that human erythrocytes were still adsorbed while bovine or porcine erythrocytes were not adsorbed. In addition, primary cell cultures of MC-Sa from mice preimmunized with bovine or porcine erythrocytes adsorbed human erythrocytes but did not adsorb bovine or porcine erythrocytes. Furthermore, studies on the different tumor fractions of MC-Sa obtained from preimmunized mice showed that, while the reaction with human erythrocytes was found only in the heavy fraction, the reaction with the species' erythrocytes used for preimmunization was present in the supernatant after centrifugation at 105,000 × g . It would appear from these studies that hemadsorption detected in MC-Sa is not due merely to the infiltration by MC-Sa of hemadsorbing spleen cells.
Abstract 2320: Long Acting Erectile Dysfunction Drug Tadalafil Limits Myocardial Ischemia/Reperfusion Injury and Preserves Left Ventricular Function through Protein Kinase G Dependent Pathway
Authors: Fadi N Salloum, Ramzi A Ockaili, Antonio Abbate, Nicholas N Hoke, Vinh Q Chau, Sumeet Lall, Rakesh C Kukreja
Journal: Circulation (2008)

Tadalafil (TAD) is a novel long acting inhibitor of phosphodiesterase-5, which enhances erectile function in men through accumulation of cGMP in the corpus cavernosum. Since cGMP-dependent protein kinase (PKG) signaling plays a key role in cardioprotection, we hypothesized that TAD would limit myocardial infarction (MI) following ischemia/reperfusion (I/R) through a mechanism involving PKG. Additionally, we contemplated that TAD would preserve left ventricular (LV) function following 30 min ischemia and 24 hr reperfusion. TAD (1 mg/kg, ip) or 10% DMSO (vehicle) was administered in ICR mice 1 hr prior to 30 min of regional ischemia by coronary artery occlusion followed by 24 hr reperfusion. In another subset of mice, KT5823 (KT), a specific PKG inhibitor (1 mg/kg, ip), was administered 10 min before TAD or 10% DMSO. Infarct size was measured at the end of reperfusion using TTC and LV function was assessed using transthoracic echocardiography. Infarct size was reduced in TAD-treated mice as compared to controls. KT abolished TAD-induced protection and KT alone had no effect on infarct size in controls (Figure ). All groups did not present with significant LV dilatation at 24 hr post infarction. However, TAD preserved fractional shortening (FS:31 ± 1.5%) as compared to control mice (FS: 22 ± 4.8%, P ± 0.05). Baseline FS was 44 ± 1.7%. KT abrogated the preservation of LV function with TAD by a marked decline in FS to 17 ± 1%. TAD is a powerful cardioprotective agent which limits MI and preserves LV function through activating PKG. Therefore, this drug may be a useful therapeutic modality to suppress I/R injury in patients with cardiovascular disease.
Vascular and upper gastrointestinal effects of non-steroidal anti-inflammatory drugs: meta-analyses of individual participant data from randomised trials
Authors: Colin Baigent, N. Bhala, J. Emberson, A. Merhi, S. Abramson, N. Arber, J. A. Baron, C. Bombardier, C. Cannon, M. E. Farkouh, G. A. FitzGerald, P. Goss, H. Halls, Ernest Hawk, C. Hawkey, C. Hennekens, M. Hochberg, L. E. Holland, P. M. Kearney, L. Laine, A. Lanas, P. Lance, A. Laupacis, J. Oates, C. Patrono, T. J. Schnitzer, S. Solomon, P. Tugwell, K. Wilson, J. Wittes, O. Adelowo, P. Aisen, A. Al-Quorain, R. Altman, G. Bakris, H. Baumgartner, C. Bresee, M. Carducci, D. M. Chang, C. T. Chou, D. Clegg, M. Cudkowicz, L. Doody, Y. El Miedany, C. Falandry, J. Farley, L. Ford, M. Garcia-Losa, M. Gonzalez-Ortiz, M. Haghighi, M. Hala, T. Iwama, Z. Jajic, D. Kerr, H. S. Kim, C. Kohne, B. K. Koo, B. Martin, C. Meinert, N. Muller, G. Myklebust, D. Neustadt, R. Omdal, S. Ozgocmen, A. Papas, P. Patrignani, F. Pelliccia, V. Roy, I. Schlegelmilch, A. Umar, O. Wahlstrom, F. Wollheim, S. Yocum, X. Y. Zhang, E. Hall, P. McGettigan, R. Midgley, R. A. Moore, R. Philipson, S. Curtis, A. Reicin, J. Bond, A. Moore, M. Essex, J. Fabule, B. Morrison, L. Tive, K. Davies, F. Yau
Journal: The Lancet (2013)
Experts Urge Early Investment in Bone Health
Authors: Tracy Hampton
Journal: JAMA (2004)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Abstract 3602: PTEN dependent angiogenesis is mainly regulated by (tumor secreted-) uPAR
Authors: Matthias Unseld, Anastasia Chilla, Clemens Pausz, Johannes Breuss, Gernot Schabbauer, Gerald Prager
Journal: Cancer Research (2014)

Abstract Tumor stroma crucially influences the growth of most epithelial tumors and is in focus of many therapeutic approaches. Several oncogenes and tumor suppressors coordinate complex signaling cascades in the tumor and its microenvironment. In this context, downregulation of the tumor suppressor PTEN was shown to be indispensable for (tumor-)angiogenesis and cancer outgrowth. However, the essential process leading to PTEN downregulation, favouring a pro-angiogenic phenotype, in tumor vasculature is unknown so far. Here we show that PTEN-dependent angiogenesis is centrally affected by tumor- secreted soluble uPAR. In detail, the presence of uPAR significantly influenced the levels of PTEN in vitro and in vivo. Endothelial cell stimulation with tumor- derived soluble uPAR remarkable decreased PTEN levels, which led to increased cell migration, enhanced wound healing as well as capillary-sprouting in vitro and enhanced endothelial cell invasion and functional vessel formation in a directed-in vivo angiogenesis assay. uPAR- derived PTEN regulation was not mediated via mRNA stability or decrease in PTEN degradation, but via regulation of PTEN transcription as revealed by actinomycin D experiments. uPAR thereby interacted with αv integrins, which enhanced FAK activation and - as a consequence - led to NFkB-dependent PTEN repression. Finally, the biological relevance of uPAR- dependent PTEN regulation was evaluated and confirmed via uPAR -/- crossbreds with either PTEN +/- or PTEN +/+ mice. Our results provide first evidence of the role of tumor-derived uPAR in regulation of the central phosphatase PTEN in angiogenic cell behaviour. Our findings give novel insights into the complex mechanism of the tumor- microenvironmental interaction and might lead to new therapeutic strategies in cancer. Citation Format: Matthias Unseld, Anastasia Chilla, Clemens Pausz, Johannes Breuss, Gernot Schabbauer, Gerald Prager. PTEN dependent angiogenesis is mainly regulated by (tumor secreted-) uPAR. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3602. doi:10.1158/1538-7445.AM2014-3602
Self-reported Sexual Function in Women and Androgen Levels
Authors: Ketan Dhatariya
Journal: JAMA (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal