Practicing What We Preach: Investigating the Role of Social Support in Sport Psychologists' Well-Being.

Abstract: Well-being and mental health of psychologists and their clients can be strongly linked to the psychologists' experience of work. We know from general theories of occupational health psychology that certain work factors will have a greater impact on well-being than others. Work engagement is positively related with occupational health, while burnout and workaholic tendencies relate negatively. An individual's resources can buffer against these negative effects. Specifically, the environmental resource of social support can impede the impact and instance of workaholism and has a positive influence on burnout. Social support is often encouraged by sport psychologists in protecting an athlete's well-being. Drawing on theory and research from work and organizational, health and social psychology we explore the lived experiences of burnout and work engagement among applied sport psychologists, investigating their perceptions of how these experiences impact their well-being. Thirty participants from five countries were asked, using semi-structured interviews, to recall specific incidents when feelings of work engagement and burnout occurred. We examined the influence of social support and its impact on these incidents. Thematic analysis revealed that burnout is frequently experienced despite high levels of work engagement. Sources of social support differ between groups of high burnout versus low burnout, as does reference to the dimensions of work engagement. Avenues for future research including investigating the role of mindfulness and therapeutic lifestyle changes for practitioners are outlined.

McCormack|Hannah M|HM|
MacIntyre|Tadhg E|TE|
O'Shea|Deirdre|D|
Campbell|Mark J|MJ|
Igou|Eric R|ER|

social support
mental health
work engagement
burnout
ethics
qualitative
self care
sport psychology


Coagulation Management During Liver Transplantation: Use of Fibrinogen Concentrate, Recombinant Activated Factor VII, Prothrombin Complex Concentrate, and Antifibrinolytics.

Abstract: Coagulation management, and transfusion practice in liver transplantation (LT) have been evolving in the recent years due to better understanding of coagulation abnormalities in end-stage liver disease, and clinical management of LT patients. Avoidance of allogeneic blood components is feasible in some patients, but multi-modal coagulation therapies may be necessary in others who develop complex coagulopathy due to hemorrhage, hemodilution, hypothermia, and acid-base disturbances. Transfusions of plasma and cryoprecipitate remain to be the mainstay therapy for procoagulant factor replacement during LT. Clinical efficacy and safety of these products are limited by logistic issues (eg, thawing), and mostly noninfectious complications. Considering potential alternatives to conventional transfusion is thus important to improve hemostatic resuscitation in complex LT cases. The present review is mainly focused on procoagulant properties of plasma and platelet transfusion, and currently available plasma-derived and recombinant factor concentrates, and antifibrinolytic agents in LT patients. The role of viscoelastic coagulation tests to guide specific component therapies will be also discussed.

Chow|Jonathan H|JH|
Lee|Khang|K|
Abuelkasem|Ezeldeen|E|
Udekwu|Obi R|OR|
Tanaka|Kenichi A|KA|

D000933:Antifibrinolytic Agents
D001779:Blood Coagulation Factors
D001780:Blood Coagulation Tests
D015942:Factor VIIa
D005340:Fibrinogen
D006801:Humans
D016031:Liver Transplantation
D017713:Platelet Transfusion
D011994:Recombinant Proteins

liver transplant
fibrinogen concentrate
prothrombin complex concentrate
coagulopathy
plasma transfusion


Combined use of meteorological drought indices at multi-time scales for improving hydrological drought detection.

Abstract: Prediction of hydrological drought in the absence of hydrological records is of great significance for water resources management and risk assessment. In this study, two meteorological drought indices, including standardized precipitation index (SPI) and standardized precipitation evapotranspiration index (SPEI) calculated at different time scales (1 to 12months), were analyzed for their capabilities in detecting hydrological droughts. The predictive skills of meteorological drought indices were assessed through correlation analysis, and two skill scores, i.e. probability of detection (POD) and false alarm rate (FAR). When used independently, indices of short time scales generally performed better than did those of long time scales. However, at least 31% of hydrological droughts were still missed in view of the peak POD score (0.69) of a single meteorological drought index. Considering the distinguished roles of different time scales in explaining hydrological droughts with disparate features, an optimization approach of blending SPI/SPEI at multiple time scales was proposed. To examine the robustness of the proposed method, data of 1964-1990 was used to establish the multiscalar index, then validate during 2000-2010. Results showed that POD exhibited a significant increase when more than two time scales were used, and the best performances were found when blending 8 time scales of SPI and 9 for SPEI, with the corresponding values of 0.82 and 0.85 for POD, 0.205 and 0.21 for FAR, in the calibration period, and even better performance in the validation period. These results far exceeded the performance of any single meteorological drought index. This suggests that when there is lack of streamflow measurements, blending climatic information of multiple time scales to jointly monitor hydrological droughts could be an alternative solution.

Zhu|Ye|Y|
Wang|Wen|W|
Singh|Vijay P|VP|
Liu|Yi|Y|

Meteorological drought
Hydrological drought
Time scales
Monitoring capability


Is the devil in the detail? A randomised controlled trial of guided internet-based CBT for perfectionism.

Abstract: An internet guided self-help cognitive-behavioural treatment (ICBT) for perfectionism was recently found to be effective (see this issue). Such studies stand in need of replication. The aim of this study was to report the outcomes and predictors of change when the treatment is delivered in a UK setting. A total of 120 people (Mean = 28.9 years; 79% female) were randomised to receive ICBT or wait-list control over 12 weeks (trial registration: NCT02756871). While there were strong similarities between the current study and its Swedish counterpart, there were also important differences in procedural details. There was a significant impact of the intervention on the primary outcome measure (Frost Multidimensional Perfectionism Scale, Concern over Mistakes subscale) and also on the Clinical Perfectionism Questionnaire (between group effect sizes d = 0.98 (95% CI: 0.60-1.36) and d = 1.04 (95% CI: 0.66-1.43) respectively using intent-to-treat analyses). Unlike the Swedish study, there was significant non-engagement and non-completion of modules with 71% of participants completing fewer than half the modules. The number of modules completed moderated the rate of change in clinical perfectionism over time. In conclusion, the study indicates the intervention is effective in a UK setting but highlighted the importance of procedural details to optimise retention.

Shafran|Roz|R|
Wade|Tracey D|TD|
Egan|Sarah J|SJ|
Kothari|Radha|R|
Allcott-Watson|Hannah|H|
Carlbring|Per|P|
Rozental|Alexander|A|
Andersson|Gerhard|G|

Internet-based
Perfectionism
Non-engagement
Completion
Moderation
Cognitive-behaviour therapy


Renal Perfusion during Hemodialysis: Intradialytic Blood Flow Decline and Effects of Dialysate Cooling.

Abstract:
BACKGROUND
Residual renal function (RRF) confers survival in patients with ESRD but declines after initiating hemodialysis. Previous research shows that dialysate cooling reduces hemodialysis-induced circulatory stress and protects the brain and heart from ischemic injury. Whether hemodialysis-induced circulatory stress affects renal perfusion, and if it can be ameliorated with dialysate cooling to potentially reduce RRF loss, is unknown.

METHODS
We used renal computed tomography perfusion imaging to scan 29 patients undergoing continuous dialysis under standard (36.5°C dialysate temperature) conditions; we also scanned another 15 patients under both standard and cooled (35.0°C) conditions. Imaging was performed immediately before, 3 hours into, and 15 minutes after hemodialysis sessions. We used perfusion maps to quantify renal perfusion. To provide a reference to another organ vulnerable to hemodialysis-induced ischemic injury, we also used echocardiography to assess intradialytic myocardial stunning.

RESULTS
During standard hemodialysis, renal perfusion decreased 18.4% (P<0.005) and correlated with myocardial injury (r=-0.33; P<0.05). During sessions with dialysis cooling, patients experienced a 10.6% decrease in perfusion (not significantly different from the decline with standard hemodialysis), and ten of the 15 patients showed improved or no effect on myocardial stunning.

CONCLUSIONS
This study shows an acute decrease in renal perfusion during hemodialysis, a first step toward pathophysiologic characterization of hemodialysis-mediated RRF decline. Dialysate cooling ameliorated this decline but this effect did not reach statistical significance. Further study is needed to explore the potential of dialysate cooling as a therapeutic approach to slow RRF decline.

Marants|Raanan|R|
Qirjazi|Elena|E|
Grant|Claire J|CJ|
Lee|Ting-Yim|TY|
McIntyre|Christopher W|CW|

D000328:Adult
D000368:Aged
D000369:Aged, 80 and over
D000704:Analysis of Variance
D003080:Cold Temperature
D018592:Cross-Over Studies
D015314:Dialysis Solutions
D005260:Female
D005500:Follow-Up Studies
D006801:Humans
D007668:Kidney
D007677:Kidney Function Tests
D008297:Male
D008875:Middle Aged
D010477:Perfusion
D012039:Regional Blood Flow
D006435:Renal Dialysis
D051436:Renal Insufficiency, Chronic
D012189:Retrospective Studies
D012720:Severity of Illness Index
D013997:Time Factors
D014057:Tomography, X-Ray Computed

hemodialysis
renal function decline
renal hemodynamics
renal ischemia


Effect of TOP2A and ERCC1 gene polymorphisms on the efficacy and toxicity of cisplatin and etoposide-based chemotherapy in small cell lung cancer patients.

Abstract:
Introduction
The main treatment regimen for small cell lung cancer (SCLC) involves platinum-based chemotherapy (cisplatin or carboplatin) and etoposide. Single nucleotide polymorphisms (SNPs) in TOP2A and ERCC1 genes were tested as prognostic and predictive factors in non-small cell lung cancer (NSCLC). There are limited data about the clinical relevance of these genetic alterations in SCLC. We undertook this retrospective study to determine the influence of SNPs in TOP2A (rs34300454; rs13695; rs11540720) and ERCC1 (rs11615; rs3212986) genes on the efficiency and toxicity of chemotherapy with platinum and etoposide in SCLC Caucasian patients.

Material and methods
The studied group included 103 Caucasian SCLC patients (65 male, 38 female, median age 65 ±7.5 years). Detailed clinical-demographical data were collected and response to treatment was monitored. DNA was isolated from peripheral blood leukocytes using QIAamp DNA Mini Kit. Single nucleotide polymorphisms were analyzed using TaqMan hydrolyzing probes in real-time PCR technique on an Eco Illumina device.

Results
Patients with C/C genotype in rs13695 of the TOP2A gene had significantly lower risk of neutropenia during chemotherapy than C/T heterozygous patients (p = 0.02, χ² = 5.51, OR = 2.676, 95% CI: 1.165-6.143). Patients harbouring homozygous C/C genotype in rs3212986 of the ERCC1 gene had significantly higher risk of anaemia during chemotherapy, than heterozygous C/A patients (p = 0.045, χ² = 4.01, OR = 0.417, 95% CI: 0.175-0.991). Furthermore, heterozygous G/A genotype in rs11615 of the ERCC1 gene was associated with significant shortening of OS (9 vs. 12 months) compared to homozygous A/A genotype (p = 0.01, χ² = 6.31, HR = 1.657, 95% CI: 1.0710-2.5633).

Conclusions
SNPs in ERCC1 and TOP2 genes may be associated with the toxicities and survival of SCLC patients treated with cisplatin and etoposide.

Nicoś|Marcin|M| Rolska-Kopińska|Anna|A| Krawczyk|Paweł|P| Grenda|Anna|A| Bożyk|Aleksandra|A| Szczyrek|Michał|M| Milanowski|Janusz|J|


Current practices in the management of adenovirus infection in allogeneic hematopoietic stem cell transplant recipients in Europe: The AdVance study.

Abstract:
OBJECTIVE
Adenovirus (AdV) infections are potentially life-threatening for allogeneic hematopoietic stem cell transplant (allo-HCT) recipients. The AdVance study aimed to evaluate the incidence, management, and outcomes of AdV infections in European allo-HCT recipients.

METHODS
As part of the study, physician surveys were conducted to determine current AdV screening and treatment practices at their center.

RESULTS
All of the 28 respondents who treat pediatric patients reported routine AdV screening practices, with 93% screening all allo-HCT recipients and others screening those with transplant-related risk factors. Nearly all centers take a pre-emptive approach to AdV treatment in both high- (89%) and low-risk patients (75%). Among the 14 respondents who treat adult patients, 5 (36%) reported routine screening practices and few (21%) screen all allo-HCT recipients unless risk factors are present. In adults, pre-emptive AdV treatment is uncommon and quantitative AdV thresholds are rare. Typical treatment for all patients with symptomatic AdV infection is off-label intravenous cidofovir.

CONCLUSIONS
Our findings confirm that screening for AdV is more common in pediatric patients. Antiviral treatment is employed in both pediatric and adult patients, although adults are generally treated when AdV disease is diagnosed. The approach to AdV screening and treatment is risk-based and consistent with clinical guidelines.

González-Vicent|Marta|M| Verna|Marta|M| Pochon|Cécile|C| Chandak|Aastha|A| Vainorius|Enrikas|E| Brundage|Tom|T| Mozaffari|Essy|E| Nichols|Garrett|G| Rao|Kanchan|K|

D018380:Hematopoietic Stem Cell Transplantation

transplantation
DNA viruses
adenoviridae
antiviral agents
infection control
virus diseases


Periprostatic nerve block alone versus combined with perineal pudendal nerve block or intrarectal local anesthesia during transrectal ultrasound-guided prostate biopsy: A prospective randomized controlled trial.

Abstract:
OBJECTIVES
To compare the efficacy, safety and cost of combinations of perineal pudendal nerve block + periprostatic nerve block and intrarectal local anesthesia + periprostatic nerve block with the standard technique (periprostatic nerve block).

METHODS
The study was designed as a randomized prospective controlled trial. Patients with elevated serum prostate-specific antigen values (prostate-specific antigen ≥4 ng/mL) and/or abnormal digital rectal examination findings were included in the study. Patients with anorectal diseases, chronic prostatitis, previous history of prostate biopsy and anorectal surgery were excluded from the study. A total of 148 patients (group 1 [periprostatic nerve block], n = 48; group 2 [intrarectal local anesthesia + periprostatic nerve block], n = 51; group 3 [perineal pudendal nerve block + periprostatic nerve block], n = 49) were included in the final analysis. Pain during insertion and manipulation of the transrectal ultrasound probe was recorded as visual analog scale 1, pain during penetration of the biopsy needle into the prostate and sampling was recorded as visual analog scale 2, and pain during the entire procedure recorded as visual analog scale 3.

RESULTS
The mean visual analog scale 1 score was significantly lower in group 3, when compared with group 1 and group 2 (P < 0.001). There was no significant difference between the groups in terms of the mean visual analog scale 2 score. The mean visual analog scale 3 score was significantly lower in group 3 when compared with other groups (P < 0.001). The total cost for transrectal ultrasound-guided biopsy in the intrarectal local anesthesia + periprostatic nerve block group was significantly higher than the other two groups.

CONCLUSIONS
The combination of perineal pudendal nerve block and periprostatic nerve block provides more effective pain control than intrarectal local anesthesia plus periprostatic nerve block and periprostatic nerve block alone, with similar complication rates and without increasing cost.

Aslan|Rahmi|R| Erbin|Akif|A| Celik|Sebahattin|S| Ucpinar|Burak|B| Eryilmaz|Recep|R| Taken|Kerem|K|

D000368:Aged
D000772:Anesthesia, Local
D000779:Anesthetics, Local
D062005:Biopsy, Large-Core Needle
D003362:Cost-Benefit Analysis
D006801:Humans
D061705:Image-Guided Biopsy
D008012:Lidocaine
D008297:Male
D008875:Middle Aged
D009407:Nerve Block
D010147:Pain Measurement
D000073818:Pain, Procedural
D011467:Prostate
D011471:Prostatic Neoplasms
D060525:Pudendal Nerve
D012007:Rectum
D018084:Ultrasonography, Interventional

periprostatic nerve block
intrarectal local anesthesia
prostate biopsy
pudendal nerve blockade


Cytochrome P450 2E1 potentiates ethanol induction of hypoxia and HIF-1α in vivo.

Abstract: Ethanol induces hypoxia and elevates HIF-1α in the liver. CYP2E1 plays a role in the mechanisms by which ethanol generates oxidative stress, fatty liver, and liver injury. This study evaluated whether CYP2E1 contributes to ethanol-induced hypoxia and activation of HIF-1α in vivo and whether HIF-1α protects against or promotes CYP2E1-dependent toxicity in vitro. Wild-type (WT), CYP2E1-knock-in (KI), and CYP2E1 knockout (KO) mice were fed ethanol chronically; pair-fed controls received isocaloric dextrose. Ethanol produced liver injury in the KI mice to a much greater extent than in the WT and KO mice. Protein levels of HIF-1α and downstream targets of HIF-1α activation were elevated in the ethanol-fed KI mice compared to the WT and KO mice. Levels of HIF prolyl hydroxylase 2, which promotes HIF-1α degradation, were decreased in the ethanol-fed KI mice in association with the increases in HIF-1α. Hypoxia occurred in the ethanol-fed CYP2E1 KI mice as shown by an increased area of staining using the hypoxia-specific marker pimonidazole. Hypoxia was lower in the ethanol-fed WT mice and lowest in the ethanol-fed KO mice and all the dextrose-fed mice. In situ double staining showed that pimonidazole and CYP2E1 were colocalized to the same area of injury in the hepatic centrilobule. Increased protein levels of HIF-1α were also found after acute ethanol treatment of KI mice. Treatment of HepG2 E47 cells, which express CYP2E1, with ethanol plus arachidonic acid (AA) or ethanol plus buthionine sulfoximine (BSO), which depletes glutathione, caused loss of cell viability to a greater extent than in HepG2 C34 cells, which do not express CYP2E1. These treatments elevated protein levels of HIF-1α to a greater extent in E47 cells than in C34 cells. 2-Methoxyestradiol, an inhibitor of HIF-1α, blunted the toxic effects of ethanol plus AA and ethanol plus BSO in the E47 cells in association with inhibition of HIF-1α. The HIF-1α inhibitor also blocked the elevated oxidative stress produced by ethanol/AA or ethanol/BSO in the E47 cells. These results suggest that CYP2E1 plays a role in ethanol-induced hypoxia, oxidative stress, and activation of HIF-1α and that HIF-1α contributes to CYP2E1-dependent ethanol-induced toxicity. Blocking HIF-1α activation and actions may have therapeutic implications for protection against ethanol/CYP2E1-induced oxidative stress, steatosis, and liver injury.

Wang|Xiaodong|X|
Wu|Defeng|D|
Yang|Lili|L|
Gan|Lixia|L|
Cederbaum|Arthur I|AI|

D056486:Chemical and Drug Induced Liver Injury

D051795:Hypoxia-Inducible Factor 1, alpha Subunit

cytochrome P450 2E1
Ethanol
HIF-1α
CYP2E1
Oxidative stress
WT
KI
knockout
KO
HIF
HIF prolyl hydroxylase 2
arachidonic acid
AA
BSO
2-methoxyestradiol
2-ME
3-NT
3-nitrotyrosine
4-HNE
4-hydroxynonenal
ALT
Free radicals
GSH
HE
HPH-2
Hepatotoxicity
IHC
LDHA
MDA
PNP
ROS
TBARS
alanine aminotransferase
hematoxylin–eosin
hypoxia-inducible factor
immunohistochemistry
knock-in
l-buthionine sulfoximine
lactate dehydrogenase A
malondialdehyde
p-nitrophenol
reactive oxygen species
reduced glutathione
thiobarbituric acid-reactive substances
wild type

P P P P P P P P P P P P P P P U U U U U U U U U U U U U U U U U U U U U U M M U U U M U U


Postweaning Grouping as a Strategy to Reduce Singly Housed Male Mice.

Abstract: Rearing laboratory mice in groups is important since social isolation after weaning induces brain alterations, which entails behavioral abnormalities in adulthood. Age is an important factor when grouping males of different litters due to inter-male aggressiveness. The aim of this study was to determine whether newly weaned mice could safely be grouped with late juvenile or early and late pubescent mice, and whether cage cleaning, the number of the hosting group members and testosterone plasma levels have any influence. Newly weaned C57BL/6J, CD1, and SCID Beige male mice were systematically grouped with same strain late juvenile, early or late pubescent male mice in clean or dirty cages of 1, 2 or 3 hosting members. We also analyzed plasma testosterone levels at different postnatal days. Our result showed that only strain and hosting male's age influence agonistic behavior toward newly weaned mice. Thus, in order not to house a recently weaned male alone, we would recommend grouping it with late juvenile same strain mice in all studied strains. In the same way, CD1 and SCID Beige pubescent mice will admit a newly weaned mouse in their group. However, we would not recommend grouping newly weaned and pubescent C57BL/6J males.

Grífols|Roger|R| Zamora|Carolina|C| Ortega-Saez|Iván|I| Azkona|Garikoitz|G|0000-0002-5312-1207

aggressiveness juvenile pubescent testosterone mouse


Trial Watch: Proteasomal inhibitors for anticancer therapy.

Abstract: The so-called "ubiquitin-proteasome system" (UPS) is a multicomponent molecular apparatus that catalyzes the covalent attachment of several copies of the small protein ubiquitin to other proteins that are generally (but not always) destined to proteasomal degradation. This enzymatic cascade is crucial for the maintenance of intracellular protein homeostasis (both in physiological conditions and in the course of adaptive stress responses), and regulates a wide array of signaling pathways. In line with this notion, defects in the UPS have been associated with aging as well as with several pathological conditions including cardiac, neurodegenerative, and neoplastic disorders. As transformed cells often experience a constant state of stress (as a result of the hyperactivation of oncogenic signaling pathways and/or adverse microenvironmental conditions), their survival and proliferation are highly dependent on the integrity of the UPS. This rationale has driven an intense wave of preclinical and clinical investigation culminating in 2003 with the approval of the proteasomal inhibitor bortezomib by the US Food and Drug Administration for use in multiple myeloma patients. Another proteasomal inhibitor, carfilzomib, is now licensed by international regulatory agencies for use in multiple myeloma patients, and the approved indications for bortezomib have been extended to mantle cell lymphoma. This said, the clinical activity of bortezomib and carfilzomib is often limited by off-target effects, innate/acquired resistance, and the absence of validated predictive biomarkers. Moreover, the antineoplastic activity of proteasome inhibitors against solid tumors is poor. In this Trial Watch we discuss the contribution of the UPS to oncogenesis and tumor progression and summarize the design and/or results of recent clinical studies evaluating the therapeutic profile of proteasome inhibitors in cancer patients.

Obrist|Florine|F| Manic|Gwenola|G| Kroemer|Guido|G| Vitale|Ilio|I| Galluzzi|Lorenzo|L|

dexamethasone
immunomodulatory drugs
ixazomib
marizomib
oprozomib
rituximab


Sleep Disturbances in Patients with Medical Conditions.

Abstract: Sleep-wake cycle disturbances are prevalent in patients with medical conditions and frequently present as part of a symptom cluster. Sleep disturbances impair functioning and quality of life, decrease adherence to treatments of the primary medical condition, and increase morbidity and mortality. The pathophysiology of sleep disturbances in these patients involves alterations in immune and neuroendocrine function and shares common pathophysiologic pathways with comorbidities such as fatigue and depression. Emphasis is placed on the evaluation and management of medical and psychiatric comorbidities and other factors contributing to sleep problems. Primary treatments include cognitive-behavioral therapy and pharmacotherapy.

Sleep disturbances
Medical conditions
Symptom cluster
Comorbidities
Cancer
Cognitive behavioral therapy
Inflammation
Insomnia


Chronic total coronary occlusion recanalization: Current techniques and new devices.

Abstract:
Percutaneous coronary intervention (PCI) of total chronic coronary occlusion (CTO) still remains a major challenge. The prevalence of a CTO has been reported to be up to 30% among patients with a clinical indication for coronary angiography. Progress has been made with further advanced interventional techniques and continuously sophisticated interventional tools. Nevertheless the number of interventions carried out to recanalize a CTO is less than 10% of all procedures. Benefits of a successful CTO recanalization include relief of angina pectoris and ischemia-related dyspnea, substantial improvement in left ventricular function and, avoidance of surgery treatment. A vast variety of new CTO PCI techniques and materials has been introduced into clinical practise and pushed success rates of reopening a CTO up to around 90% in experienced hands. Particulary the introduction of the retrograde technique was a milestone. New developed microcatheters and special polymer coated wires allow to recanalize via small collaterals and vessels. Other tools such as intravascular ultrasound (IVUS) and multislice computertomography (MSCT) help to identify the anatomy and the characteristic of the lesions. Any invasive cardiac center should adopt CTO PCI procedures as standard therapy.

OBJECTIVE
This review wants to assess and describe the latest development in CTO recanalization strategies.

Gülker|Jan-Erik|JE|
Bansemir|Lars|L|
Klues|Heinrich G|HG|
Bufe|Alexander|A|

Latest development
Recanalization strategies
Chronic total occlusion
Coronary artery disease
Technical innovation


Is Redshirting Beneficial for Reading Acquisition Success?

Abstract: The controversy around the effect of academic redshirting on reading acquisition continues receiving attention in the international literature. However, few studies are known with non-English speaking children. In this study we intend to understand this phenomenon with 698 Portuguese speaking first graders, 360 girls (51.6%), aged between 5 years old and 8 months and 7 years old and 6 months (M = 6.3 months, SD = 3.9 months). Reading acquisition precursors were assessed namely phonemic awareness and letter-sound knowledge. Results reveal that 5.9% of first graders are redshirted. Clusters analysis indicated two clusters per variable. Cluster 1 with low phonemic awareness and letter-sound knowledge and low socioeconomic status, cluster 2 with high phonemic awareness and letter-sound knowledge and medium-high socioeconomic status. The cluster results suggest a prevalence of 24.5% children at risk of having learning difficulties. The MANOVA indicated that only socioeconomic status has an effect on phonemic awareness and letter-sound knowledge, with children from medium-high level presenting higher results. It is concluded that redshirting did not bring additional advantages for reading acquisition success. Implications about the importance of education in order to lessen those differences, as well as prevent difficulties are presented.

Sucena|Ana|A|
Marques|Cátia|C|
Silva|Ana Filipa|AF|
Garrido|Cristina|C|
Pimenta|Rui|R|

redshirting
reading acquisition
phonemic awareness
letter-sound knowledge
reading precursors


Rhomboidal Pt(II) metallacycle-based NIR-II theranostic nanoprobe for tumor diagnosis and image-guided therapy.

Abstract: Fluorescent theranostics probes at the second near-IR region (NIR-II; 1.0-1.7 µm) are in high demand for precise theranostics that minimize autofluorescence, reduce photon scattering, and improve the penetration depth. Herein, we designed and synthesized an NIR-II theranostic nanoprobe 1 that incorporates a Pt(II) metallacycle 2 and an organic molecular dye 3 into DSPE-mPEG5000 (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000]). This design endows 1 with good photostability and passive targeting ability. Our studies show that 1 accurately diagnoses cancer with high resolution and selectively delivers the Pt(II) metallacycle to tumor regions via an enhanced permeability and retention effect. In vivo studies reveal that 1 efficiently inhibits the growth of tumor with minimal side effects. At the same time, improved fluorescent imaging quality and signal-to-noise ratios are shown due to the long emission wavelengths. These studies demonstrate that 1 is a potential theranostic platform for tumor diagnosis and treatment in the NIR-II region.

Sun|Yue|Y|
Ding|Feng|F|
Zhou|Zhixuan|Z|
Li|Chonglu|C|
Pu|Maoping|M|
Xu|Yuling|Y|
Zhan|Yibei|Y|
Lu|Xiaoju|X|
Li|Haibing|H|
Yang|Guangfu|G|
Sun|Yao|Y|
Stang|Peter J|PJ|

theranostic
image-guided therapy
metallacycle
second near-infrared region
supramolecular coordination complexes


Sibling screening in suspected abusive head trauma: a proposed guideline.

Abstract: Abusive head trauma (AHT) is the leading cause of death from child abuse in children younger than 5 years. It is well documented that the infant contacts of children presenting with suspected AHT are at an increased risk of abuse when compared to the general infant population. Despite this association, a paucity of literature stratifies this risk and translates it to the clinic such that this high-risk group is stringently screened for abusive injuries. In this light, the authors propose a standardised screening method for all contact children of the index case and call for further consensus on the subject.

Mankad|Kshitij|K|
Sidpra|Jai|J|
Oates|Adam J|AJ|
Calder|Alistair|A|
Offiah|Amaka C|AC|
Choudhary|Arabinda|A|

Siblings
Screening
Abusive head trauma
Child abuse
Children
Infants


Postprandial High-Resolution Impedance Manometry Identifies Mechanisms of Nonresponse to Proton Pump Inhibitors.

Abstract:
BACKGROUND & AIMS
Recognition of rumination and supragastric belching is often delayed as symptoms may be mistakenly attributed to gastroesophageal reflux disease. However, distinct from gastroesophageal reflux disease, rumination and supragastric belching are more responsive to behavioral interventions than to acid-suppressive and antireflux therapies. Postprandial high-resolution impedance manometry (PP-HRIM) is an efficient method to identify rumination and belches. We investigated the distribution of postprandial profiles determined by PP-HRIM, and identified patient features associated with postprandial profiles among patients with nonresponse to proton pump inhibitors (PPIs).

METHODS
We performed a retrospective analysis of PP-HRIM studies performed on 94 adults (mean age, 50.6 y; 62% female) evaluated for PPI nonresponsiveness at an esophageal referral center, from January 2010 through May 2016. Following a standard esophageal manometry protocol, patients ingested a solid refluxogenic test meal (identified by patients as one that induces symptoms) with postprandial monitoring up to 90 minutes (median, 50 min). Patients were assigned to 1 of 4 postprandial profiles: normal; reflux only (>6 transient lower esophageal sphincter relaxations (TLESRs)/h); supragastric belch (>2 supragastric belches/h), with or without TLESR; or rumination (≥1 rumination episode/h) with or without TLESR and supragastric belching. The primary outcome was postprandial profile.

RESULTS
Of the study participants, 24% had a normal postprandial profile, 14% had a reflux-only profile, 42% had a supragastric belch profile, and 20% had a rumination profile. In multinomial regression analysis, the rumination group most frequently presented with regurgitation, the supragastric belch and rumination groups were younger in age, and the reflux-only group had a lower esophagogastric junction contractile integral. The number of weakly acidic reflux events measured by impedance-pH monitoring in patients receiving PPI therapy was significantly associated with frequency of rumination episodes and supragastric belches.

CONCLUSIONS
In a retrospective analysis of 94 nonresponders to PPI therapy evaluated by PP-HRIM, we detected an abnormal postprandial pattern in 76% of cases: 42% of these were characterized as supragastric belching, 20% as rumination, and 14% as reflux only. Age, esophagogastric junction contractility, impedance-pH profiles, and symptom presentation differed significantly among groups. PP-HRIM can be used in the clinic to evaluate mechanisms of PPI nonresponse.

Yadlapati|Rena|R|
Tye|Michael|M|
Roman|Sabine|S|
Kahrilas|Peter J|PJ|
Ritter|Katherine|K|
Pandolfino|John E|JE|

D019959:Feeding and Eating Disorders of Childhood


Abstract:
Renal replacement therapy (RRT) for acute kidney injury (AKI) patients in an intensive care unit (ICU) presents unique problems of providing biochemical and fluid removal in patients with unstable circulations, inotropes, and increased capillary permeability. Although no individual modality has been shown to confer a mortality benefit, it is assumed that continuous therapies like peritoneal dialysis (PD) and venovenous hemofiltration or hemodiafiltration may be better tolerated by the patient with hemodynamic instability, raised intracranial pressure (ICP), and liver failure. An individual patient may require more than one treatment in the course of his/her illness. The therapies offered may reflect available resources, local expertise, and cost constraints.

How to cite this article
Lobo VA. Renal Replacement Therapy in Acute Kidney Injury: Which Mode and When? Indian J Crit Care Med 2020;24(Suppl 3):S102-S106.

Peritoneal dialysis
Continuous renal replacement therapy
Sustained low-efficiency daily dialysis


Anodal transcranial pulsed current stimulation: A novel technique to enhance corticospinal excitability.

Abstract:
OBJECTIVE
We aimed to compare the effects of anodal-transcranial pulsed current stimulation (a-tPCS) with conventional anodal transcranial direct current stimulation (a-tDCS) on corticospinal excitability (CSE) in healthy individuals.

METHODS
CSE of the dominant primary motor cortex of the resting right extensor carpi radialis muscle was assessed before, immediately, 10, 20 and 30min after application of four experimental conditions: (1) a-tDCS, (2) a-tPCS with short inter-pulse interval (a-tPCSSIPI, 50ms), (3) a-tPCS with long inter-pulse interval (a-tPCSLIPI., 650ms) and (4) sham a-tPCS. The total charges were kept constant in all experimental conditions except sham condition. The outcome measure in this study was motor evoked potentials.

RESULTS
Only a-tDCS and a-tPCSSIPI (P<0.05) induced significant increases in CSE, lasted for at least 30min. Post-hoc tests indicated that this increase was larger in a-tPCSSIPI (P<0.05). There were no significant changes following application of a-tPCSLIPI and sham a-tPCS. All participants tolerated the applied currents in all experimental conditions very well.

CONCLUSIONS
Compared to a-tDCS, a-tPCSSIPI is a better technique for enhancement of CSE. There were no sham effects for application of a-tPCS. However, unlike a-tDCS which modifies neuronal excitability by tonic depolarization of the resting membrane potential, a-tPCS modifies neuronal excitability by a combination of tonic and phasic effects.

SIGNIFICANCE
a-tPCS could be considered as a promising neuromodulatory tool in basic neuroscience and as a therapeutic technique in neurorehabilitation.

Transcranial pulsed current stimulation
Corticospinal excitability
Transcranial direct current stimulation
Neuroplasticity
Non-invasive brain stimulation


Perceptions of Informal and Formal Coping Strategies for Intimate Partner Violence Among Gay and Bisexual Men.

Abstract: The prevalence of intimate partner violence (IPV) in same-sex male relationships has been reported to be at least as prevalent as is observed in female-male relationships. Though research has focused on understanding the prevalence and antecedents of IPV in male-male relationships, there is a paucity of data describing perceptions of coping strategies adopted by gay and bisexual men who may experience IPV. Ten focus group discussions were conducted with 64 gay and bisexual men in Atlanta, Georgia, between September 2013 and November 2013. Focus groups examined perceptions of how gay and bisexual men would respond to IPV and the IPV-coping services they would utilize. Thematic analysis was conducted to identify themes that describe how gay and bisexual men perceive existing IPV services and how they would use these services, if gay and bisexual men were to experience IPV. The results indicate that men experiencing IPV in male-male relationships do not have adequate access to IPV services that are tailored to their unique needs. As a result, there is a strong reliance on informal sources of support. Services are urgently needed to meet the unique needs of men experiencing IPV in same-sex relationships.


Idiopathic Anaphylaxis: A Form of Mast Cell Activation Syndrome.

Abstract: Idiopathic anaphylaxis is a condition caused by paroxysmal episodes of sudden-onset multiorgan involvement variably including laryngeal edema, urticaria, bronchoconstriction, dyspnea, hypoxia, abdominal pain, nausea, vomiting, diarrhea, and hypotension. Rarely, the episodes can lead to cardiovascular collapse and death in the absence of a clear trigger, especially in the presence of other cardiovascular comorbidities. Elevated mast cell mediators such as tryptase and histamine have been reported during episodes, and mast cells are considered the primary cells responsible for driving anaphylaxis in humans. Basophils also secrete histamine and LTC4 when activated and theoretically can contribute to symptoms. As our understanding of mast cell disorders continue to grow, the classification for these disorders evolves. The purpose of this article was 2-fold: to review the epidemiology, clinical manifestations, and diagnosis of idiopathic anaphylaxis and to discuss the classification of idiopathic anaphylaxis within the broader context of mast cell activation disorders.

Giannetti|Matthew P|MP|
Akin|Cem|C|
Castells|Mariana|M|

Idiopathic anaphylaxis
Anaphylaxis
Mast cell activation syndrome
Mast cells
Tryptase
Mastocytosis


Distributed nestmate recognition in ants.

Abstract: We propose a distributed model of nestmate recognition, analogous to the one used by the vertebrate immune system, in which colony response results from the diverse reactions of many ants. The model describes how individual behaviour produces colony response to non-nestmates. No single ant knows the odour identity of the colony. Instead, colony identity is defined collectively by all the ants in the colony. Each ant responds to the odour of other ants by reference to its own unique decision boundary, which is a result of its experience of encounters with other ants. Each ant thus recognizes a particular set of chemical profiles as being those of non-nestmates. This model predicts, as experimental results have shown, that the outcome of behavioural assays is likely to be variable, that it depends on the number of ants tested, that response to non-nestmates changes over time and that it changes in response to the experience of individual ants. A distributed system allows a colony to identify non-nestmates without requiring that all individuals have the same complete information and helps to facilitate the tracking of changes in cuticular hydrocarbon profiles, because only a subset of ants must respond to provide an adequate response.

nestmate recognition
distributed systems
cuticular hydrocarbons
natural computing


Reasons and predictors for antiretroviral therapy change among HIV-infected adults at South West Ethiopia.

Abstract:
OBJECTIVE
This retrospective cohort study is aimed to assess reasons and predictors of regimen change from initial highly active antiretroviral therapy among 1533 Human Immunodeficiency virus-infected adult patients at the Jimma University Tertiary Hospital.

RESULTS
One in two (47.7%) adults changed their antiretroviral therapy regimen. Patients who were above the primary level of education [Hazard ratio (HR) 1.241 (95% CI 1.070-1.440)] and with human immunodeficiency virus/tuberculosis co-infection [HR 1.405 (95% CI 1.156-1.708)] had the higher risk of regimen change than their comparator. Individuals on Efavirenz [HR 0.675 (95% CI 0.553-0.825)] and non-stavudine [HR 0.494 (95% CI 0.406-0.601)] based regimens had lower risk of regimen change.

Mekonnen|Endalkachew|E| Workicho|Abdulhalik|A| Hussein|Nezif|N| Feyera|Teka|T|

D023241:Antiretroviral Therapy, Highly Active

Initial highly active antiretroviral therapy


Registration of Aerial Optical Images with LiDAR Data Using the Closest Point Principle and Collinearity Equations.

Abstract: Registration of large-scale optical images with airborne LiDAR data is the basis of the integration of photogrammetry and LiDAR. However, geometric misalignments still exist between some aerial optical images and airborne LiDAR point clouds. To eliminate such misalignments, we extended a method for registering close-range optical images with terrestrial LiDAR data to a variety of large-scale aerial optical images and airborne LiDAR data. The fundamental principle is to minimize the distances from the photogrammetric matching points to the terrestrial LiDAR data surface. Except for the satisfactory efficiency of about 79 s per 6732 × 8984 image, the experimental results also show that the unit weighted root mean square (RMS) of the image points is able to reach a sub-pixel level (0.45 to 0.62 pixel), and the actual horizontal and vertical accuracy can be greatly improved to a high level of 1/4⁻1/2 (0.17⁻0.27 m) and 1/8⁻1/4 (0.10⁻0.15 m) of the average LiDAR point distance respectively. Finally, the method is proved to be more accurate, feasible, efficient, and practical in variety of large-scale aerial optical image and LiDAR data.

registration
LiDAR
collinearity equation
point cloud
aerial Image


Modelling atmospheric oxidation of 2-aminoethanol (MEA) emitted from post-combustion capture using WRF-Chem.

Abstract: Carbon capture and storage (CCS) is a technological solution that can reduce the amount of carbon dioxide (CO2) emissions from the use of fossil fuel in power plants and other industries. A leading method today is amine based post-combustion capture, in which 2-aminoethanol (MEA) is one of the most studied absorption solvents. In this process, amines are released to the atmosphere through evaporation and entrainment from the CO2 absorber column. Modelling is a key instrument for simulating the atmospheric dispersion and chemical transformation of MEA, and for projections of ground-level air concentrations and deposition rates. In this study, the Weather Research and Forecasting model inline coupled with chemistry, WRF-Chem, was applied to quantify the impact of using a comprehensive MEA photo-oxidation sequence compared to using a simplified MEA scheme. Main discrepancies were found for iminoethanol (roughly doubled in the detailed scheme) and 2-nitro aminoethanol, short MEA-nitramine (reduced by factor of two in the detailed scheme). The study indicates that MEA emissions from a full-scale capture plant can modify regional background levels of isocyanic acid. Predicted atmospheric concentrations of isocyanic acid were however below the limit value of 1 ppbv for ambient exposure. The dependence of the formation of hazardous compounds in the OH-initiated oxidation of MEA on ambient level of nitrogen oxides (NOx) was studied in a scenario without NOx emissions from a refinery area in the vicinity of the capture plant. Hourly MEA-nitramine peak concentrations higher than 40 pg m(-3) did only occur when NOx mixing ratios were above 2 ppbv. Therefore, the spatial variability and temporal variability of levels of OH and NOx need to be taken into account in the health risk assessment. The health risk due to direct emissions of nitrosamines and nitramines from full-scale CO2 capture should be investigated in future studies.

Karl|M|M|
Svendby|T|T|
Walker|S-E|SE|
Velken|A S|AS|
Castell|N|N|
Solberg|S|S|

2-Aminoethanol
Isocyanic acid
Health risk
Nitramines
CO(2)-capture
WRF–Chem


Zilpaterol hydrochloride lowers marbling score by dilution of marbling and depression of intramuscular adipocyte volume in M. longissimus dorsi of beef steers.

Abstract: We hypothesized that zilpaterol hydrochloride (ZH) depresses marbling scores in beef cattle by dilution of the intramuscular (i.m.) adipose tissue in the longissimus thoracis (LT) by increased muscle cross-sectional area. Crossbred steers were treated with ractopamine hydrochloride (RH; 28 d) or ZH (20 d) or no β-adrenergic agonist (CON). ZH increased LT cross-sectional area (P < .001) and depressed rib fat depth (P < .001), USDA marbling score (P < .05), percent LT lipid (P < .02), total lipid in a 2.54 cm-thick LM steak (P < .05) and most LT lipid fatty acids (P ≤ .05),but had no effect on mean i.m. adipocyte volume (P = .15). ZH increased the relative volume proportion of smaller i.m. adipocytes (250 to 500 pL) and decreased the proportion of larger i.m. adipocytes (2000 and 3000 pL) (P < .05). We conclude that ZH reduces marbling score both by dilution of the marbling and a depression of i.m. adipocyte relative volumes.

Gonzales|Carolina L|CL|
Lawrence|Ty E|TE|
Hutcheson|John P|JP|
Smith|Stephen B|SB|

Marbling score
Intramuscular adipocytes
β-Adrenergic agonists
Intramuscular lipid


Femoral cannulation for cardiopulmonary bypass with a novel bidirectional perfusion cannula.

Abstract: The use of the novel bidirectional femoral cannula is described in this video tutorial. We demonstrate the percutaneous cannulation and decannulation of the femoral artery for cardiopulmonary bypass in a patient undergoing minimally invasive mitral valve surgery. The procedure itself is presented step by step for each important phase. Finally, we report the postoperative course following the successful use of a peripheral bidirectional cannula.

Kahrovic|Amila|A| Werner|Paul|P| Angleitner|Philipp|P| Wiedemann|Dominik|D| Laufer|Günther|G| Andreas|Martin|M|

D019060:Minimally Invasive Surgical Procedures

Cardiopulmonary bypass
Percutaneous cannulation
Femoral artery
Bidirectional cannula
Minimally invasive surgery


Complete agenesis of dorsal pancreas with pancreatic cyst: A case report.

Abstract: Agenesis of the dorsal pancreas (ADP) is extremely rare disease with no specific symptoms and there is no clear pathogenesis. Approximately half of the affected individuals develop diabetes resulting from reduced islet cell mass secondary to lack of endocrine structures. In this case, we aimed to present a 17-year-old female patient with ADP accompanied by a pancreatic cyst.

Ustabasioglu|Fethi Emre|FE|
Kurt|Nazmi|N|
Tuncbilek|Nermin|N|

Agenesis of dorsal pancreas
pancreatic cyst
magnetic resonance imaging


Ozone-Induced Aryl Hydrocarbon Receptor Activation Controls Lung Inflammation via Interleukin-22 Modulation.

Abstract: Airborne ozone exposure causes severe lung injury and inflammation. The aryl hydrocarbon Receptor (AhR) (1), activated in pollutant-induced inflammation, is critical for cytokine production, especially IL-22 and IL-17A. The role of AhR in ozone-induced lung inflammation is unknown. We report here that chronic ozone exposure activates AhR with increased tryptophan and lipoxin A4 production in mice. AhR-/- mice show increased lung inflammation, airway hyperresponsiveness, and tissue remodeling with an increased recruitment of IL-17A and IL-22-expressing cells in comparison to control mice. IL-17A- and IL-22-neutralizing antibodies attenuate lung inflammation in AhR-/- and control mice. Enhanced lung inflammation and recruitment of ILC3, ILC2, and T cells were observed after T cell-specific AhR depletion using the AhRCD4cre-deficient mice. Together, the data demonstrate that ozone exposure activates AhR, which controls lung inflammation, airway hyperresponsiveness, and tissue remodeling via the reduction of IL-22 expression.

Michaudel|Chloé|C|
Bataille|Florent|F|
Maillet|Isabelle|I|
Fauconnier|Louis|L|
Colas|Cyril|C|
Sokol|Harry|H|
Straube|Marjolène|M|
Couturier-Maillard|Aurélie|A|
Dumoutier|Laure|L|
van Snick|Jacques|J|
Quesniaux|Valérie F|VF|
Togbe|Dieudonnée|D|
Ryffel|Bernhard|B|

D051792:Basic Helix-Loop-Helix Transcription Factors


Multivariate Statistical Approach to Image Quality Tasks.

Abstract: Many existing Natural Scene Statistics-based no reference image quality assessment (NR IQA) algorithms employ univariate parametric distributions to capture the statistical inconsistencies of bandpass distorted image coefficients. Here we propose a multivariate model of natural image coefficients expressed in the bandpass spatial domain that has the potential to capture higher-order correlations that may be induced by the presence of distortions. We analyze how the parameters of the multivariate model are affected by different distortion types, and we show their ability to capture distortion-sensitive image quality information. We also demonstrate the violation of Gaussianity assumptions that occur when locally estimating the energies of distorted image coefficients. Thus we propose a generalized Gaussian-based local contrast estimator as a way to implement non-linear local gain control, that facilitates the accurate modeling of both pristine and distorted images. We integrate the novel approach of generalized contrast normalization with multivariate modeling of bandpass image coefficients into a holistic NR IQA model, which we refer to as multivariate generalized contrast normalization (MVGCN). We demonstrate the improved performance of MVGCN on quality relevant tasks on multiple imaging modalities, including visible light image quality prediction and task success prediction on distorted X-ray images.

Gupta|Praful|P|0000-0000-0000-000X
Bampis|Christos G|CG|
Glover|Jack L|JL|
Paulter|Nicholas G|NG|
Bovik|Alan C|AC|

image quality assessment
generalized contrast normalization
X-ray images
multivariate statistical modeling


Whole body MRI in paediatric oncology.

Abstract: Whole body MRI provides excellent contrast resolution imaging and is an interesting alternative to nuclear medicine examinations in paediatric oncology because it does not involve exposure to radiation. This technique, now feasible in clinical practice, helps to evaluate metastatic spread and response to treatment, which are of great prognostic interest. Numerous studies have demonstrated the non-inferiority of this technique when compared to nuclear medicine examinations. However, there is still a need to standardize indications in each type of cancer and at every stage of it. This article first discusses the technical principles of whole body MRI, then reviews current clinical applications for the modality in children, and finally, discusses future useful developments for paediatric oncology.

Canale|S|S| Vilcot|L|L| Ammari|S|S| Lemery|M|M| Bidault|F|F| Balleyguier|C|C| Caramella|C|C| Dromain|C|C|

Whole body MRI
Paediatric
Oncology
Tumour evaluation


Interim Guidelines on Antiviral Therapy for COVID-19.

Abstract: Since the first case was reported in Wuhan, Hubei Province, China on December 12, 2019, Coronavirus disease 2019 (COVID-19) has spread widely to other countries since January 2020. As of April 16, 2020, 10635 confirmed cases have been reported, with 230 deaths in Korea. COVID-19 patients may be asymptomatic or show various clinical manifestations, including acute symptoms such as fever, fatigue, sore throat; pneumonia presenting as acute respiratory distress syndrome; and multiple organ failure. As COVID-19 has such varied clinical manifestations and case fatality rates, no standard antiviral therapy regimen has been established other than supportive therapy. In the present guideline, we aim to introduce potentially helpful antiviral and other drug therapies based on in vivo and in vitro research and clinical experiences from many countries.


Evaluation of high rate ponds operational and design strategies for algal biomass production and domestic wastewater treatment.

Abstract: This study evaluated the effect of high rate ponds (HRPs) depth on algal biomass production during domestic wastewater treatment. HRPs were evaluated for 20, 30, and 40 cm depths, with and without CO2 supplementation. In addition, 40 cm deep HRP with ultraviolet (UV) pre-disinfection was evaluated. The concentration of chlorophyll-a as a function of time for each evaluated condition was represented by logistic models that were after submitted to cluster analysis. The 20 cm HRPs presented higher chlorophyll-a concentration, reaching a maximum of 5.8 and 4.3 mg L-1, in the HRPs with and without CO2 addition, respectively. Ammonia nitrogen and soluble phosphorus were greater removed in shallower HRPs. The addition of CO2 influenced the nutrient removal processes, optimizing nutrient recovery by biomass assimilation. HRP configuration did not influence organic matter removal (~40% of removal efficiency in all HRPs), predominant microalgae genera (Chlorella sp. and Scenedesmus), and E. coli inactivation (removal of ~2 log units), except for the 20 cm HRP without CO2 that had removal of 4 log units due to high pH values. For HRPs with CO2 addition and UV pre-disinfection, the models for 40 cm were grouped together with those obtained for 30 cm HRPs, indicating the same behavior for chlorophyll-a production as a function of time. Thus, it can be concluded that the evaluated strategies represent alternatives for reducing HRP area requirements. Moreover, results may represent advancement and major contributions for HRP design criteria.

Couto|Eduardo|E|
Calijuri|Maria Lúcia|ML|
Assemany|Paula|P|
Cecon|Paulo Roberto|PR|

Depth
Microalgae
UV pre-disinfection
CO(2) supplementation
Domestic sewage
Open algae ponds


Great Auricular Neuralgia: Case Series.

Abstract:
BACKGROUND/OBJECTIVE
The great auricular nerve (GAN) arises from C2-C3 and provides innervation over the skin in the pre-auricular region, jaw angle, posteroinferior pinna, and mastoid. Although damage to the GAN has been reported following trauma or procedures nearby this nerve course, neuralgia of this nerve is uncommon with knowledge based on a handful of case reports in literature. The objective of this study is to describe the presentation, treatment, and outcome of 13 cases of GAN neuralgia.

METHODS
Case series. Retrospecive review of charts from 1994 to 2018 with diagnoses: "auricular neuralgia," "auricular neuritis," or "auricular neuropathy." We included subjects with neuralgic pain within the distribution of the GAN, and excluded patients with atypical facial pain, GAN neuropathy, or unclear etiology.

RESULTS
Of 79 charts, 13 patients met criteria (age at onset 11-59; 11 women, 2 men). Pain was most often described as paroxysmal stabbing provoked by: turning the head (n = 7), touching the neck (n = 5), neck position during sleep (n = 2), jaw movement (n = 2), and other (n = 2). Seven patients received GAN blocks: all noted dramatic improvement in pain, including 3 who continued to receive serial blocks at our institution successfully for the next 2 to 5 years. Two patients successfully transitioned from GAN blocks to GAN stimulators. One patient with GAN lymphoma had resolution of pain following GAN resection.

CONCLUSION
GAN neuralgia should be considered in the differential for periauricular pain. GAN blocks or stimulators may be helpful for pain management.

Duvall|Jaclyn R|JR|
Garza|Ivan|I|
Kissoon|Narayan R|NR|
Robertson|Carrie E|CE|

neuralgia
great auricular nerve
nerve block
neuropathic pain
peripheral nerve stimulation
red ear syndrome


Ectopic Thymus: An Unusual Case of Subglottic Mass.

Abstract:
OBJECTIVES
The aim of our study is to report a case of a child with subglottic thymus that was suspected during diagnostic work-up for severe airway obstruction, excised surgically and confirmed with final histopathological examination. Moreover, we performed a narrative literature review to outline clinical and diagnostic features of this rare condition and to report suggestions for the management of subglottic masses.

METHODS
We report the case of a 7-month-old boy who was admitted to our Pediatric Airway Team Unit due to a history of worsening biphasic stridor and recurrent episodes of upper airway obstruction. The successful diagnostic work-up and a narrative literature of analogous cases of subglottic thymus were reported.

RESULTS
Ectopic thymus is a very rare condition in which thymic tissue is found outside the normal pathway of its embryonic migration. It usually presents as a cystic or, more rarely, solid mass, showing an indolent course toward spontaneous involution. In some cases, however, it becomes symptomatic exerting compression on surrounding vital structures. Due to its rarity, the initial diagnosis is normally mistaken with inflammatory diseases or malignancies and the definitive diagnosis is only achieved after histological examination of the excised specimen. To our knowledge, only four other cases of subglottic ectopic thymic tissue have been reported in the English literature so far and the diagnosis has never been suspected preoperatively.

CONCLUSION
It is mandatory to consider ectopic thymic tissue in the differential diagnosis in children presenting with airways obstruction in order to prevent unnecessary, extensive, and exploratory surgery.


Plasma vitamin D biomarkers and leukocyte telomere length.

Abstract: Vitamin D may reduce telomere shortening through anti-inflammatory and anti-cell proliferation mechanisms. In the present study, we examined the association between vitamin D and relative leukocyte telomere length by using both plasma 25-hydroxyvitamin D (25(OH)D) and 1,25-dihydroxyvitamin D (1,25(OH)2D) biomarkers. Vitamin D biomarker levels and leukocyte telomere length were measured using plasma samples collected in 1989-1990 from participants of the Nurses' Health Study, a study of nurses from 11 US states. In total, 1,424 participants had their 25(OH)D levels assessed and 837 had their 1,25(OH)2D levels assessed. Genotyping was performed on 480 participants on 12 single nucleotide polymorphisms in vitamin D-related genes. Linear and logistic regression models were used. Higher 25(OH)D levels were significantly associated with longer telomere length (P for trend = 0.05), and the odds ratio increased from 1.07 (P = 0.65) when comparing the second lowest quartile of 25(OH)D with the lowest to 1.59 (P = 0.01) when comparing the highest quartile with the lowest. Vitamin D-related single nucleotide polymorphisms and 1,25(OH)2D levels were not significantly associated with telomere length. Total calcium intake significantly modified the association between 25(OH)D and telomere length (P for interaction = 0.05). Higher plasma 25(OH)D levels may be associated with longer telomeres, and this association may be modified by calcium intake.

Liu|Jason J|JJ|
Prescott|Jennifer|J|
Giovannucci|Edward|E|
Hankinson|Susan E|SE|
Rosner|Bernard|B|
Han|Jiali|J|
De Vivo|Immaculata|I|


The complete mitochondrial genome of the Honduran white bat Ectophylla alba (Allen 1982) (Chiroptera: Phyllostomidae).

Abstract: The Honduran white bat, Ectophylla alba (Allen 1982), is one of eight species belonging to the family Phyllostomidae that exclusively roosts in tents. Due to its restricted distribution, habitat specificity, and diet requirements, E. alba has been strongly affected by habitat loss and fragmentation during the last decade. In this study, we developed the first genomic resource for this species; we assembled and analyzed in detail the complete mitochondrial genome of E. alba. The mitogenome of E. alba is 16,664 bp in length and is comprised of 13 protein coding genes (PCGs), 2 ribosomal RNA genes, 22 transfer RNA genes (tRNAs), and a putative Control Region (CR) 1,232 bp in length. Gene arrangement in the mitochondrial chromosome of E. alba is identical to that reported before in other species of co-familiar bats. All PCGs are under purifying selection, with atp8 experiencing the least selective pressure. In all PCGs, codons ending with adenine are preferred over others ending in thymine and cytosine. Except tRNA-Serine 1, all tRNAs exhibit a cloverleaf secondary structure. The CR of E. alba exhibits three domains commonly described in other mammals, including bats; extended terminal associated sequences (ETAS), central, and conserved sequence block (CSB). A ML phylogenetic reconstruction of the family Phyllostomidae based on all 13 mitochondrial PCGs confirms the monophyletic status of the subfamily Sternodermatinae and indicates the close relationship between E. alba and the genus Artibeus. This is the first genomic resource developed for E. alba and represents the first step to improving our understanding of the genomic underpinnings involved in the evolution of specialization as well as acclimatization and adaptation to local and global change of specialist bats.

Vivas-Toro|Isabela|I|
Ortega|Jorge|J|
Baeza|J Antonio|JA|

Mitogenome
tRNAs
Control region
Secondary structure
Stenodermatinae
Tent-roosting bat


Dietary Gluten as a Conditioning Factor of the Gut Microbiota in Celiac Disease.

Abstract: The gut microbiota plays a relevant role in determining an individual's health status, and the diet is a major factor in modulating the composition and function of gut microbiota. Gluten constitutes an essential dietary component in Western societies and is the environmental trigger of celiac disease. The presence/absence of gluten in the diet can change the diversity and proportions of the microbial communities constituting the gut microbiota. There is an intimate relation between gluten metabolism and celiac disease pathophysiology and gut microbiota; their interrelation defines intestinal health and homeostasis. Environmental factors modify the intestinal microbiota and, in turn, its changes modulate the mucosal and immune responses. Current evidence from studies of young and adult patients with celiac disease increasingly supports that dysbiosis (i.e., compositional and functional alterations of the gut microbiome) is present in celiac disease, but to what extent this is a cause or consequence of the disease and whether the different intestinal diseases (celiac disease, ulcerative colitis, Crohn disease) have specific change patterns is not yet clear. The use of bacterial-origin enzymes that help completion of gluten digestion is of interest because of the potential application as coadjuvant in the current treatment of celiac disease. In this narrative review, we address the current knowledge on the complex interaction between gluten digestion and metabolism, celiac disease, and the intestinal microbiota.

Bascuñán|Karla A|KA|
Araya|Magdalena|M|
Roncoroni|Leda|L|
Doneda|Luisa|L|
Elli|Luca|L|

gluten
gut
microbiota
celiac disease
gluten-free diet


Breast Cancer: Exploring the Facts and Holistic Needs during and beyond Treatment.

Abstract:
Breast cancer patients face challenges throughout the journey of diagnosis, treatment, post-treatment, and recovery. The breast cancer patient is exposed to a multidisciplinary team including doctors, nurses, therapists, counselors, and psychologists. While the team assembled together aims to address multiple facets in breast cancer care, the sub-specialized nature of individual professional practices may constrain the overview of patients' holistic needs and a comprehensive approach to cancer management. This paper aims to provide an overview of the holistic needs of breast cancer patients at each stage of their cancer journey, addressing their complex physical, psychological, and social needs. As every patient is different, cancer care has to be tailored to each patient based on a holistic needs assessment. This paper also explores how support can be provided from the perspectives of the healthcare providers, family members and caretakers. Examples of general practices at healthcare institutions worldwide as well as supportive care provided by support groups are discussed. The needs of breast cancer patients extend beyond the resolution of cancer as a disease, and the restoration of health as far as possible is a critical component of healing. Understanding the complex issues involved in the journey of breast cancer will aid healthcare providers to be better equipped to sensitively address their concerns and focus on healing the patient holistically.

METHODOLOGY
This paper provides a literature review of validated practices in different countries and elaborates on the holistic needs of patients at various stages of recovery. This review is based on more than a decade of publications sourced from multiple resources including PubMed journal articles; books and official websites of breast cancer organizations.

Ng|Zhi Xuan|ZX|
Ong|Mei Shan|MS|
Jegadeesan|Tamilarasi|T|
Deng|Shuo|S|
Yap|Celestial T|CT|

breast cancer
holistic needs
cancer survivorship


Overexpression of Aiolos in Peripheral Blood Mononuclear Cell Subsets from Patients with Systemic Lupus Erythematosus and Rheumatoid Arthritis.

Abstract: Genetic studies demonstrate that the Aiolos polymorphisms contribute to the susceptibility to autoimmune diseases. The purpose of the study was to investigate the Aiolos expression in lymphocytes and monocytes in the peripheral blood from patients with SLE and RA, and to explore the correlation between Aiolos expression in cell subsets and laboratory measurements. Peripheral blood mononuclear cells (PBMC) from 32 patients with SLE, 35 patients with RA, and 37 healthy controls were purified. Aiolos expression in PBMC subsets was examined by flow cytometry. In SLE patients, a much higher percentage of Aiolos + CD8+ T cells and Aiolos + CD14+ monocytes was found, when compared with healthy controls (p = 8.29 × 10(-5) and p = 1.01 × 10(-5), respectively). Furthermore, the percentage of CD4+ and CD8+ T cells, CD19+ B cells, and CD14+ monocytes expressing Aiolos in RA patients was also determined and each found higher than that in healthy controls (p = 0.009, p = 4.11 × 10(-5), p = 0.001, and p = 1.11 × 10(-5), respectively). The percentage of Aiolos + CD8+ T cells was weakly correlated with ESR in SLE patients and RF in RA patients (r s = 0.37, p = 0.038; r s = 0.34, p = 0.044, respectively). On the other hand, the percentage of Aiolos + CD14+ monocytes was significantly correlated with multiple laboratory measurements, including ESR, creatinine, CRP, LDH, proteinuria, albumin, and ACCPA in patients (r s = 0.62, p < 0.001; r s = 0.65, p < 0.001; r s = 0.44, p = 0.010; r s = 0.42, p = 0.022; r s = 0.52, p = 0.013; r s = 0.34, p = 0.048, respectively). To our knowledge, it is the first study to demonstrate overexpression of Aiolos in PBMC subsets in SLE and RA patients. The results indicate that overexpression of Aiolos may contribute to pathogenesis of SLE and RA.

Cai|Xinze|X| Liu|Xudong|X| Du|Shuyan|S| Xu|Xiaoxue|X| Liu|Ang|A| Ge|Xin|X| Qiao|Ying|Y| Jiang|Yi|Y|

Aiolos
Mononuclear cell
Systemic lupus erythematosus
Rheumatoid arthritis


Role of Rotation in Total Ankle Replacement.

Abstract:
BACKGROUND
The importance of total ankle replacement (TAR) implant orientation in the axial plane is poorly understood with major variation in surgical technique of implants on the market. Our aim was to better understand the axial rotational profile of patients undergoing TAR.

METHODS
In 157 standardized computed tomography (CT) scans of patients with end-stage ankle arthritis planning to undergo primary TAR surgery, we measured the relationship between the knee posterior condylar axis, the tibial tuberosity, the transmalleolar axis (TMA), and the tibiotalar angle. The foot position was measured in relation to the TMA with the foot plantigrade. The variation between the medial gutter line and the line bisecting both gutters was assessed.

RESULTS
The mean external tibial torsion was 34.5 ± 10.3 degrees (11.8-62 degrees). When plantigrade, the mean foot position relative to the TMA was 21 ± 10.6 degrees (0.7-38.4 degrees) internally rotated. As external tibial torsion increased, the foot position became more internally rotated relative to the TMA (Pearson correlation, 0.6; P < .0001). As the tibiotalar angle became more valgus, the foot became more externally rotated relative to the TMA (Pearson correlation, -0.4; P < .01). The mean difference between the medial gutter line and a line bisecting both gutters was 4.9 ± 2.8 degrees (1.7-9.4 degrees). More than 51% of patients had a difference greater than 5 degrees. The mean angle between the medial gutter line and a line perpendicular to the TMA was 7.5 ± 2.6 degrees (2.8-13.7 degrees).

CONCLUSION
There was a large variation in rotational profile of patients undergoing TAR, particularly between the medial gutter line and the TMA. Surgeon designers and implant manufacturers should develop consistent methods to guide surgeons toward judging the appropriate axial rotation of their implant on an individual basis. We recommend careful clinical assessment and preoperative CT scans to enable the correct rotation to be determined.

LEVEL OF EVIDENCE
Level IIc, outcomes research.

arthritis gutter alignment biomechanics deformity impingement loosening


Remote ischaemic preconditioning down-regulates kinin receptor expression in neutrophils of patients undergoing heart surgery.

Abstract:
OBJECTIVES
Remote ischaemic preconditioning (RIPC) may protect distant organs against ischaemia-reperfusion injury. We investigated the impact of RIPC on kinin receptor expression in neutrophils following RIPC in patients undergoing coronary artery bypass grafting (CABG).

METHODS
Patients undergoing elective CABG with cardiopulmonary bypass (CPB) were randomized to RIPC (n = 15) or control (n = 15) groups. The study group underwent RIPC by inflation of a blood pressure cuff on the arm. Expression of kinin receptors, plasma concentrations of IL-6, IL-8, IL-10, TNF-α and neutrophil elastase were determined at baseline (before RIPC/sham), immediately before surgery (after RIPC/sham) and 30 min and 24 h after surgery. Plasma bradykinin levels were assessed before and after RIPC/sham, and at 30 min, 6, 12 and 24 h after surgery. Serum creatine kinase (CK), troponin I, C-reactive protein (CRP) and lactate levels were measured immediately prior to surgery and 30 min, 6, 12, 24 and 48 h after surgery.

RESULTS
Kinin B2 receptor expression did not differ between the groups at baseline (pre-RIPC), but was significantly lower in the RIPC group than in the control group after RIPC/sham (P < 0.05). Expressions of both kinin B1 and B2 receptors were significantly down-regulated in the RIPC group, and this persisted to 24 h after surgery (P < 0.001). Neutrophil elastase levels were significantly increased after surgery. There were no differences in CK, CRP, cytokine, lactate or troponin I levels between the groups.

CONCLUSIONS
RIPC down-regulated the expression of kinin B1 and B2 receptors in neutrophils of patients undergoing CABG.

Saxena|Pankaj|P|
Aggarwal|Shashi|S|
Misso|Neil L|NL|
Passage|Jurgen|J|
Newman|Mark A J|MA|
Thompson|Philip J|PJ|
d'Udekem|Yves|Y|
Praporski|Slavica|S|
Konstantinov|Igor E|IE|

Ischaemic preconditioning
Kinin receptors
Bradykinin
Cardiac surgery
Ischaemia


Morphological and mechanical characterization of 3D printed PLA scaffolds with controlled porosity for trabecular bone tissue replacement.

Abstract: Bone transplant is still the gold standard approach when dealing with orthopedic trauma or disease. When this solution is not possible, scaffolding is a possibility provided by bone tissue engineering. To support the regeneration process, damaged bone tissue is removed and replaced by porous scaffold structures. In recent years, additive manufacturing has shown huge potential to produce scaffold structures with the required performance. In the current work, PLA scaffolds with different designs were 3D printed, using optimal manufacturing parameters. Scaffolds with three different porosity values were obtained by changing the filament offset from 571 to 1333 μm. A total of twelve designs were tested under monotonic and dynamic compression conditions. Numerical analysis showed good correlation with experimental results, allowing for a better assessment of scaffold mechanical behavior. Stress relaxation was measured on four different strain levels, assessing scaffold's behavior after implantation and consequent static response over time. Overall, orthogonal design provided better performance, due to improved material deposition. With lower porosity scaffolds equilibrium stress reached 24 MPa after 300 s relaxation time under 4% deformation, and the obtained equilibrium modulus was 428 MPa. Overall, attained results show that 3D printing with PLA can be applied in the manufacture of scaffolds for trabecular bone replacement.

D000071556:Cancellous Bone
D011091:Polyesters
D016062:Porosity
D066330:Printing, Three-Dimensional
D023822:Tissue Engineering
D054457:Tissue Scaffolds

3D printing
Scaffolds
Mechanical behavior
Stress relaxation
Bone replacement
Morphological characterization


Photodynamic effects on human periodontal-related cells in vitro.

Abstract:
BACKGROUND
Photodynamic therapy (PDT) may be especially effective in combination with conventional periodontal therapy by its antimicrobial activities, but PDT may also exhibit other mechanisms that promote the healing of periodontal tissue. Therefore, the purpose of the present study was to evaluate the photodynamic effect of PDT on human periodontal ligament cells (hPDLCs) and human gingival fibroblasts (hFBs) in vitro and other possible mechanisms to promote periodontal healing.

METHODS
The proliferation of hPDLCs and hFBs was assessed by MTT assay. Cell attachment on cementum slices of hPDLCs and hFBs was evaluated by MTT assay. Type I collagen synthesis of hPDLCs and hFBs was analyzed using enzyme linked immunosorbent assay. The alkaline phosphatase (ALP) activity in hPDLCs was measured by p-nitrophenyl phosphate substrate reactions.

RESULTS
PDT treatment induced constant time-dependent growth of hPDLCs and hFBs at 24h, 72h and 6 days (P<0.05). PDT treatment also promoted time-dependent hPDLCs and hFBs attachment on the cementum slices at 24h, 72h and 6 days compared to the controlled cells (P<0.05). Type I collagen synthesis of hPDLCs and hFBs was markedly stimulated by PDT in a time-dependent manner (P<0.05). Likewise, a significant increase in the specific ALP activity in hPDLCs was observed (P<0.05).

CONCLUSIONS
The findings of this study indicate that PDT exhibited no cytotoxicity to hPDLCs or hFBs. Instead, it stimulated proliferation, attachment and collagen synthesis of hPDLCs and hFBs and ALP activity of hPDLCs. These effects might signal similar PDT activity on periodontal-related cells, and expanding the scope of its potential therapeutic utilization is very appealing.

Qiao|Jing|J| Wang|Shiying|S| Wen|Yi|Y| Jia|Huimei|H|

Photodynamic therapy
Periodontal therapy
Human periodontal ligament cells
Human gingival fibroblasts


Ten myths (or facts?) about workaholism: An appetitive motivation framework.

Abstract: This commentary intends to provide constructive input into the "Ten myths about work addiction" by Griiffiths et al. (2018). I place the information into an appetitive motivation theoretical lens of addiction as well as outline the kernels of truth associated with each myth. Advancement of an understanding of the underlying mechanisms of addiction demands consideration that any number of appetitive-associated behaviors might become disrupted - including those at the workplace.

myths
appetitive motivation
work addiction


Prognostic accuracy of different disseminated intravascular coagulation criteria in critically ill adult patients: a protocol for a systematic review and meta-analysis.

Abstract:
INTRODUCTION
Disseminated intravascular coagulation (DIC) is a common and serious condition that can lead to poor outcomes in critically ill patients. To make a correct diagnosis and improve the outcome of patients with DIC, several organisations have put forward DIC scoring systems. However, which criteria is the best to use for diagnosing DIC remains a continuing controversy even though many studies have been conducted to validate the diagnostic accuracy of each DIC scoring system.

METHODS AND ANALYSIS
We will conduct a systematic review and meta-analysis of the diagnostic accuracy of DIC criteria for the prediction of mortality in critically ill adult patients. The primary objective is to assess the predictive values of the DIC criteria of Japanese Association for Acute Medicine, International Society on Thrombosis and Haemostasis, Japanese Ministry of Health and Welfare, Korean Society on Thrombosis and Hemostasis and Chinese Diagnostic Scoring System for 28-day mortality. We will search electronic bibliographic databases such as MEDLINE, EMBASE and Cochrane Central Register of Controlled Trials. Two reviewers will independently screen titles and abstracts, perform full article reviews and extract study data. We will report study characteristics and assess methodological quality using the Quality of Diagnostic Accuracy Studies-II tool. If pooling is appropriate, we will compute parameter estimates using bivariate random-effects and hierarchical summary receiver operating characteristic models to produce summary receiver operating curves, summary operating points (pooled sensitivity and specificity) and 95% confidence regions around the summary operating points. Clinical and methodological subgroup and sensitivity analyses will be performed to explore heterogeneity.

ETHICS AND DISSEMINATION
This systematic review will help physicians diagnose DIC accurately and improve their clinical practice in critically ill settings. Approval from an ethics committee is not required. The findings will be disseminated through publication in a peer-reviewed journal.

PROSPERO REGISTRATION NUMBER
CRD42017079350.

Yoshimura|Jumpei|J| Yamakawa|Kazuma|K|0000-0003-2999-4021 Kodate|Akira|A| Kodate|Mari|M| Fujimi|Satoshi|S|

disseminated Intravascular coagulation
critically Ill patients
diagnosis
mortality
sepsis


Enhanced anti-tumor and anti-metastasis efficacy against breast cancer with an intratumoral injectable phospholipids-based phase separation gel co-loaded with 5-fluotouracil and magnesium oxide by neutralizing acidic microenvironment.

Abstract: Traditional chemotherapy against breast cancer usually accompanied with metastasis. Thus, it is significant to enhance anti-tumor and anti-metastasis efficacy simultaneously. The extracellular environment of most tumors is acidic with pH 6.5-6.9, which play an important role in driving promotion of tumor metastasis. Herein, we developed an intratumoral injectable phospholipids-based phase separation gel (PPSG) co-loaded with 5-fluotouracil (5FU) and magnesium oxide (MgO), termed as 5FU + MgO-PPSG. It was prepared by simple mixing, and showed in the appearance of a clear and yellow solution with good fluidity. After intratumoral injection, PPSG system achieved phase transition and sustained released alkaline substance to neutralize acidic microenvironment in tumor site. Both in vitro and in vivo research showed a strong anti-tumor and anti-metastasis efficacy. 4T1-bearing mice after administration with 5FU + MgO-PPSG was achieved significantly prolonged survival time, compared with other groups. In conclusion, the novel 5FU + MgO-PPSG system might have a great potential to improve treatment method against breast metastasis.

Yang|Liuqing|L| Song|Xu|X| Gong|Ting|T| Wang|Luyao|L| Zhao|Ting|T| Li|Lin|L| Zhou|Chuchu|C| Sun|Xun|X| Zhang|Zhirong|Z| Gong|Tao|T|

D000818:Animals
D000970:Antineoplastic Agents
D045744:Cell Line, Tumor
D002470:Cell Survival
D016503:Drug Delivery Systems
D005260:Female
D005472:Fluorouracil
D005782:Gels
D006863:Hydrogen-Ion Concentration
D008277:Magnesium Oxide
D008325:Mammary Neoplasms, Experimental
D008807:Mice, Inbred BALB C
D010743:Phospholipids
D047368:Tumor Burden
D014945:Wound Healing

5-Fluotouracil
Magnesium oxide
pH
Tumor metastasis
Phospholipid-based phase separation gel


The impact of inflow treatment for claudicants with both aortoiliac and femoropopliteal occlusive disease.

Abstract:
PURPOSE
This study evaluated the early and long-term results of the sole use of endovascular treatment in the treatment of inflow lesions in claudicants with both aortoiliac and femoropopliteal (FP) lesions.

METHODS
A retrospective study that included 100 limbs in 73 patients was performed. The patency rates of aortoiliac artery stents, the continued clinical improvement rates, the risk factors for persistent disabling claudication after inflow revascularization, and the rates of freedom from additional FP procedures were examined.

RESULT
After inflow revascularization, almost complete relief from intermittent claudication was seen in 79 % of the limbs, while 21 % of the limbs continued to suffer from disabling claudication. A multivariate analysis showed that a run-off score of ≥7 was an independent predictor for persistent disabling claudication after aortoiliac revascularization [hazard ratio (HR) 5.11, 95 % confidence interval (CI) 1.34-19.45; P = 0.02]. The primary patency rates at 1, 3, 5, and 6 years were 96, 96, 96 and 89 %, respectively. The secondary patency rate at 6 years was 100 %. The continued clinical improvement rates at 1, 3, 5, and 6 years were 81, 78, 78 and 72 %, respectively. The rates of freedom from additional FP procedures at 1, 3, 5, and 6 years were 97, 90, 90, and 90 %, respectively.

CONCLUSIONS
Aortoiliac endovascular revascularization is effective treating claudicants with both aortoiliac and femoropopliteal lesions. Furthermore, a run-off score of ≥7 appears to be a potential predictor for persistent disabling claudication.

Maekawa|Takashi|T| Komori|Kimihiro|K| Kodama|Akio|A| Banno|Hiroshi|H| Narita|Hiroshi|H| Sugimoto|Masayuki|M|

D000368:Aged
D000369:Aged, 80 and over
D001011:Aorta
D001157:Arterial Occlusive Diseases
D015331:Cohort Studies
D057510:Endovascular Procedures
D005260:Female
D005263:Femoral Artery
D006801:Humans
D007083:Iliac Artery
D007383:Intermittent Claudication
D008297:Male
D008875:Middle Aged
D011150:Popliteal Artery
D012189:Retrospective Studies
D012307:Risk Factors
D015607:Stents
D013997:Time Factors
D016896:Treatment Outcome
D014654:Vascular Patency

Claudication
Endovascular therapy
Peripheral arterial disease


Antinociceptive and anti-inflammatory effect of Poincianella pyramidalis (Tul.) L.P. Queiroz.

Abstract:
ETHNOPHARMACOLOGY RELEVANCE
Poncianella pyramidalis (Leguminosae) is a Caatinga plant used in folk medicine because of its pharmacological properties, which include anti-inflammatory action. However, chemical compounds responsible for this effect have not yet been identified.

AIM OF THE STUDY
This study aimed to evaluate the antioxidant, antinociceptive and anti-inflammatory effects of the ethyl acetate fraction from the inner bark of P. pyramidalis.

MATERIAL AND METHODS
Total phenol content (TP) was estimated using the Folin-Ciocalteu reagent, while in vitro antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Chemical identification was done using LC-PDA/MS and LC-ESI/MS/MS. In vivo antinociceptive and anti-inflammatory properties were investigated using formalin, mechanical hypernociception and carrageenan-induced pleurisy assays in mice.

RESULTS
TP was 525.08 ± 17.49 μg mg-1 gallic acid equivalent. The ethyl acetate fraction (EAF) inhibited 87.76% of the DPPH radical with an EC50 of 22.94 μg mL-1 and Antioxidant Activity Index of 1.74. LC-PDA/MS and LC-ESI/MS/MS identified 15 compounds that are mostly derived from gallic and ellagic acids. Regarding in vivo antinociceptive and anti-inflammatory activity, EAF (100 mg kg-1) significantly reduced the nociceptive response in the second phase of the formalin assay by 50% (p < 0.01) compared with the control group. In the hypernociception test, a significant (p < 0.001) anti-hyperalgesic effect of EAF (100 mg kg-1) was observed up to the third hour of evaluation (p < 0.001). In the carrageenan assay, EAF (100 mg kg-1) was shown to inhibit protein extravasation, increase total leukocytes and neutrophils, and inhibit mononuclear cells.

CONCLUSION
These results demonstrate EAF from the inner bark of P. pyramidalis has strong in vitro antioxidant effect as well as in vivo antinociceptive and anti-inflammatory activities, which may be attributed to the bark being rich in phenolic compounds derived from gallic acid.

Quintans|Jullyana de Souza Siqueira|JSS|

Poincianella Phenolic Hypernociception Carrageenan Hyperalgesic


Hyperbaric oxygen-induced long non-coding RNA MALAT1 exosomes suppress MicroRNA-92a expression in a rat model of acute myocardial infarction.

Abstract: Hyperbaric oxygen (HBO) improves angiogenesis. The effect of HBO on metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), a pro-angiogenic long non-coding RNA, in cardiac myocyte-derived exosomes and acute myocardial infarction (AMI) is unknown. We aimed to investigate whether MALAT1 is altered in cardiac myocyte-derived exosomes in response to HBO as well as the molecular regulatory mechanisms of MALAT1 in cardiac myocytes treated with HBO. Cardiac myocytes were cultured, and HBO was applied at 2.5 atmosphere absolute in a hyperbaric chamber. Exosomes were extracted from the culture media. A rat model of AMI generated by the ligation of the left anterior descending artery was used. HBO significantly increased MALAT1 expression in cardiac myocytes and HBO-induced MALAT1 and exosomes attenuated miR-92a expression after myocardial infarction. Expression of krüppel-like factor 2 (KLF2) and CD31 was significantly decreased after infarction and HBO-induced exosomes significantly reversed the expression. Silencing of MALAT1 using MALAT1-locked nucleic acid GapmeR significantly attenuated KLF2 and CD31 protein expression after infarction induced by HBO-induced exosomes. HBO-induced exosomes also decreased infarct size significantly. HBO-induced exosomes from cardiac myocytes up-regulate MALAT1 to suppress miR-92a expression and counteract the inhibitory effect of miR-92a on KLF2 and CD31 expression in left ventricular myocardium after myocardial infarction to enhance neovascularization.

Shyu|Kou-Gi|KG|0000-0002-5710-8640 Wang|Bao-Wei|BW| Fang|Wei-Jen|WJ| Pan|Chun-Ming|CM| Lin|Chiu-Mei|CM|

D019408:Platelet Endothelial Cell Adhesion Molecule-1

MALAT1
exosome
myocardial infarction
hyperbaric oxygen


Carcass gain per kg feed intake: developing a stakeholder-driven benchmark for comparing grow-finishing pig performance.

Abstract: Feed conversion ratio (FCR) in grow-finishing pigs is one of the most important determinants of pig farm profitability and production efficiency. In its simplest form, FCR represents the amount of feed used per unit weight gain of the pig. Yet, this approach entails various limitations hampering its practical applicability such as availability of accurate data and large variation in ways to adapt FCR values for different starting and end weight as well as mortality rates. Various stakeholders are using their own formulas to determine FCR creating a 'definition nonconformity' when comparing FCRs among farms. This study aimed to optimize the calculation of FCR through the use of participatory qualitative research. A multidisciplinary research group of 9 persons (animal scientists, veterinarians and agricultural economists) and a consulting group of 31 stakeholders (representing the Flemish primary sector, feed industry, pharma, genetic companies, large retailers, academia and policy institutions) were involved. The decision problem analysis started with a literature review, followed by 25 in-depth interviews and their analyses (NVivo 11™). This led to an additional literature review and the formation of focus (expert) groups that helped to formulate preliminary FCR formulas. Revision rounds between the research team and the stakeholders further fine-tuned the formulas with the final result being two distinct complimentary formulas that are fit for purpose. Both refer to carcass gain per kg feed intake (plain (CGF) and standardized (CGFstandardized)). The first formula (CGF), namely ${{{\it{number \, delivered \, pigs}} \times {\it{average \, warm \, carcass \, weight}} - {\it{number \, stocked \, piglets}} \times {\it{average \, piglet \, weight}} \times {\it{piglet \, carcass \, yield}}} \over {{\it{feed \, consumption}}}}$ is an objective representation of the animals' performance. The second formula (CGFstandardized) was developed for farm benchmarking, incorporating a seven-step standardization process that corrects for mortality and 'standardizes' for a fixed (yet fictive) live weight trajectory of 25 to 115 kg. This second formula allows to compare farms (or batches of fattening pigs) with different weight trajectories and different mortality rates. A webtool was designed to ease this standardization process (https://varkensloket.be/tools/CGF).

farm management
feed efficiency
participatory research
pig production
standardized formula


Exploring semi-supervised variational autoencoders for biomedical relation extraction.

Abstract: The biomedical literature provides a rich source of knowledge such as protein-protein interactions (PPIs), drug-drug interactions (DDIs) and chemical-protein interactions (CPIs). Biomedical relation extraction aims to automatically extract biomedical relations from biomedical text for various biomedical research. State-of-the-art methods for biomedical relation extraction are primarily based on supervised machine learning and therefore depend on (sufficient) labeled data. However, creating large sets of training data is prohibitively expensive and labor-intensive, especially so in biomedicine as domain knowledge is required. In contrast, there is a large amount of unlabeled biomedical text available in PubMed. Hence, computational methods capable of employing unlabeled data to reduce the burden of manual annotation are of particular interest in biomedical relation extraction. We present a novel semi-supervised approach based on variational autoencoder (VAE) for biomedical relation extraction. Our model consists of the following three parts, a classifier, an encoder and a decoder. The classifier is implemented using multi-layer convolutional neural networks (CNNs), and the encoder and decoder are implemented using both bidirectional long short-term memory networks (Bi-LSTMs) and CNNs, respectively. The semi-supervised mechanism allows our model to learn features from both the labeled and unlabeled data. We evaluate our method on multiple public PPI, DDI and CPI corpora. Experimental results show that our method effectively exploits the unlabeled data to improve the performance and reduce the dependence on labeled data. To our best knowledge, this is the first semi-supervised VAE-based method for (biomedical) relation extraction. Our results suggest that exploiting such unlabeled data can be greatly beneficial to improved performance in various biomedical relation extraction, especially when only limited labeled data (e.g. 2000 samples or less) is available in such tasks.

D000465:Algorithms
D035843:Biomedical Research
D057225:Data Mining
D004347:Drug Interactions
D016571:Neural Networks, Computer
D060066:Protein Interaction Maps
D000069553:Supervised Machine Learning

Variational autoencoder
Relation extraction
Biomedical literature
Semi-supervised learning


Antibacterial and anti-biofilm activity, and mechanism of action of pleurocidin against drug resistant Staphylococcus aureus.

Abstract: The abuse of antibiotics has resulted in the emergence of multi-drug-resistant bacteria. Staphylococcus aureus is a frequent cause of infections, and antibiotic-resistant S. aureus has become a serious problem. Antimicrobial peptides play an important role in innate immunity and are attracting increasing attention as alternative antibiotics. In a previous study, pleurocidin, derived from winter flounder, was identified as a 25-amino acid antimicrobial peptide with no cytotoxicity toward mammalian cells and low hemolytic activity. In the present study, pleurocidin was observed to exhibit antimicrobial activity against gram-positive and gram-negative bacteria, especially against drug resistant S. aureus. Pleurocidin retained its antibacterial activity against drug resistant S. aureus in the presence of a physiological salt concentration. Membrane depolarization assays and propidium iodide uptake indicated that pleurocidin kills bacteria by damaging the integrity of the bacterial membrane. DNA binding assays revealed that pleurocidin binds to DNA. Thus, pleurocidin targets not only the bacterial membrane, but also their DNA. S. aureus biofilms have become a serious problem because of increased resistance to antibiotics. Therefore, we investigated the effect of pleurocidin on biofilm inhibition and eradication using crystal violet staining and microscopic observation. Pleurocidin inhibited and eradicated biofilms at low concentrations. Taken together, the results suggested that pleurocidin is a promising candidate therapeutic agent to treat drug-resistant bacteria and biofilm-related infections.

Ko|Su Jin|SJ|
Kang|Na Hee|NH|
Kim|Min Kyung|MK|
Park|Jonggwan|J|
Park|Eunji|E|
Park|Ga Hyeon|GH|
Kang|Tae Woo|TW|
Na|Da Eun|DE|
Park|Jin Bae|JB|
Yi|Yae Eun|YE|
Jeon|Seung Hun|SH|
Park|Yoonkyung|Y|

D000900:Anti-Bacterial Agents
D018441:Biofilms
D002462:Cell Membrane
D004247:DNA
D029941:Fish Proteins
D005840:Gentian Violet
D008564:Membrane Potentials
D050296:Microbial Viability
D008853:Microscopy
D011485:Protein Binding
D013194:Staining and Labeling
D013211:Staphylococcus aureus

Drug resistant Staphylococcus aureus
Antimicrobial peptide
Physiological salt
Biofilm


Peptide amphiphile delivery of sonic hedgehog protein promotes neurite formation in penile projecting neurons.

Abstract: Erectile dysfunction (ED) critically impacts quality of life in prostatectomy, diabetic and aging patients. The underlying mechanism involves cavernous nerve (CN) damage, resulting in ED in 80% of prostatectomy patients. Peptide amphiphile (PA) nanofiber hydrogel delivery of sonic hedgehog (SHH) protein to the injured CN, improves erectile function by 60% at 6 weeks after injury, by an unknown mechanism. We hypothesize that SHH is a regulator of neurite formation. SHH treatment promoted extensive neurite formation in uninjured and crushed CNs, and SHH inhibition decreased neurites >80%. Most abundant neurites were observed with continuous SHH PA treatment of crushed CNs. Once induced with SHH, neurites continued to grow. SHH rescued neurite formation when not given immediately. SHH is a critical regulator of neurite formation in peripheral neurons under uninjured and regenerative conditions, and SHH PA treatment at the time of injury/prostatectomy provides an exploitable avenue for intervention to prevent ED.

Dobbs|Ryan|R|
Choe|Shawn|S|
Kalmanek|Elizabeth|E|
Harrington|Daniel A|DA|
Stupp|Samuel I|SI|
McVary|Kevin T|KT|
Podlasek|Carol A|CA|

Sonic hedgehog
Neurite formation
Cavernous nerve injury
Pelvic ganglia
Peptide amphiphile nanofiber hydrogel
Peripheral nerve regeneration
Schwann cells


Preliminary Study of Dynamic Cerebral Autoregulation in Acute Ischemic Stroke: Association With Clinical Factors.

Abstract: Background and Purpose: Dynamic cerebral autoregulation (dCA) is probably impaired in the acute and even subacute phases after acute ischemic stroke (AIS); however, the relationship between relevant clinical factors and dCA after AIS has not been investigated. The identification of possible determinants may therefore provide potential therapeutic targets to improve dCA in AIS. Methods: This study enrolled 67 consecutive patients diagnosed with AIS within 3 days from symptom onset. Serial measurements were performed 1-3 days (measurement 1) and 7-10 days (measurement 2) after the onset. Middle cerebral artery blood flow velocities and simultaneous arterial blood pressure (ABP) were recorded continuously with transcranial Doppler combined with a servo-controlled finger plethysmograph. Transfer function analysis was used to derive dCA parameters, phase difference (PD), and coherence in low-frequency range (0.06-0.12 Hz). Univariate and multivariate linear regression analyses were conducted to determine the relationship between clinical factors and PD within the two time points of measurements. Multivariate logistic regression was performed to reveal the relationship between PD and clinical outcomes. Results: Bilateral PD was significantly lower (indicating impaired dCA) in AIS patients, both in measurement 1 and measurement 2 when compared with those of healthy controls (all P < 0.001). After controlling for relevant clinical factors, in measurement 1, age (β = -0.29, P = 0.01), recombinant tissue plasminogen activator (rt-PA) intravenous thrombolysis (β = 0.25, P = 0.034), subtype of large-artery atherosclerosis (LAA) (β = -0.31, P = 0.007), and uric acid level (β = -0.32, P = 0.009) were significant independent predictors of ipsilateral PD. In measurement 2, subtype of LAA (β = -0.28, P = 0.049) and uric acid level (β = -0.43, P = 0.005) were still significant predictive values for ipsilateral PD. After adjusting for age and National Institutes of Health Stroke Scale at admission, ipsilateral PD >35.37° in measurement 2 was independent predictor of good clinical outcomes (adjusted OR = 6.97, 95% CI: 1.27-38.14, P = 0.03). Conclusion: DCA was sustained to be bilaterally impaired in the acute and even subacute phase after AIS. Patients who receiving rt-PA thrombolysis tended to have a better dCA in the acute phase. Increasing age, subtype of LAA, and higher uric acid level had prognostic value for disturbed autoregulation. A relatively preserved dCA may predict good clinical outcomes.

Ma|Hongyin|H| Guo|Zhen-Ni|ZN| Jin|Hang|H| Yan|Xiuli|X| Liu|Jia|J| Lv|Shan|S| Zhang|Peng|P| Sun|Xin|X| Yang|Yi|Y|

dynamic cerebral autoregulation
acute ischemic stroke
clinical factors
transfer function analysis
rt-PA thrombolysis