Bacterial community response to petroleum contamination in brackish tidal marsh sediments in the Yangtze River Estuary, China.

Abstract: The brackish tidal marsh in the Baimaosha area of the Yangtze River Estuary was severely contaminated by 400 tons of heavy crude petroleum from a tanker that sank in December 2012. The spill accident led to severe environmental damage owing to its high toxicity, persistence and wide distribution. Microbial communities play vital roles in petroleum degradation in marsh sediments. Therefore, taxonomic analysis, high-throughput sequencing and 16S rRNA functional prediction were used to analyze the structure and function of microbial communities among uncontaminated (CK), lightly polluted (LP), heavily polluted (HP), and treated (TD) sediments. The bacterial communities responded with increased richness and decreased diversity when exposed to petroleum contamination. The dominant class changed from Deltaproteobacteria to Gammaproteobacteria after petroleum contamination. The phylum Firmicutes increased dramatically in oil-enriched sediment by 75.78%, 346.19% and 267.26% in LP, HP and TD, respectively. One of the suspected oil-degrading genera, Dechloromonas, increased the most in oil-contaminated sediment, by 540.54%, 711.27% and 656.78% in LP, HP and TD, respectively. Spore protease, quinate dehydrogenase (quinone) and glutathione-independent formaldehyde dehydrogenase, three types of identified enzymes, increased enormously with the increasing petroleum concentration. In conclusion, petroleum contamination altered the community composition and microorganism structure, and promoted some bacteria to produce the corresponding degrading enzymes. Additionally, the suspected petroleum-degrading genera should be considered when restoring oil-contaminated sediment.

Wang|Min|M| Sha|Chenyan|C| Wu|Jian|J| Su|Jinghua|J| Wu|Jianqiang|J| Wang|Qing|Q| Tan|Juan|J| Huang|Shenfa|S|

Bacterial community
Petroleum contamination
Brackish marsh sediment
Gene enzyme


Evaluation of digital cryotherapy using a commercially available sleeve style ice boot in healthy horses and horses receiving i.v. endotoxin.

Abstract:
BACKGROUND
Continuous digital cryotherapy experimentally prevents development and reduces severity of sepsis-associated laminitis. A sleeve style ice boot where ice is in direct contact with the skin, and water drains from the boot is being used clinically for distal limb cryotherapy. The degree of cooling achieved by this boot is unknown.

OBJECTIVES
Evaluate skin and lamellar cooling after application of the ice sleeve in healthy horses, and the same horses during an endotoxaemia model.

STUDY DESIGN
Prospective study, crossover design.

METHODS
In eight healthy horses thermocouples were inserted into dorsal lamellae of both front feet, and under skin on both metacarpi. One forelimb received cryotherapy using sleeve style ice boot, with contralateral limb as control. Temperature was recorded on data logging devices at 5 min intervals during each cryotherapy session. Day 1: temperature data was collected for healthy horses. Day 2: data was collected for the same horses during i.v. administration of endotoxin.

RESULTS
In healthy and endotoxaemic horses, the sleeve style ice boot significantly decreased mean skin (7.2°C and 5.8°C respectively) and lamellar (10.8°C and 9.6°C respectively) temperatures compared with control limbs (P<0.001). Skin and lamellar temperatures in endotoxaemic horses undergoing cryotherapy were significantly colder than in healthy horses (P = 0.01).

MAIN LIMITATIONS
Order of treatment not randomised.

CONCLUSIONS
The boot caused significant decreases in lamellar temperatures compared with untreated control limbs in all horses. Endotoxaemic horses had significantly colder lamellae and skin than healthy horses. This study is the first to show that a sleeve style boot, where ice does not cover the hoof, can cause significant decreases in lamellar temperatures through cooling of blood as it travels to the foot.

digital cryotherapy
horse
sepsis-associated laminitis
lamellar temperatures
sleeve-style ice boot


Ammonium is the preferred source of nitrogen for planktonic foraminifer and their dinoflagellate symbionts.

Abstract: The symbiotic planktonic foraminifera Orbulina universa inhabits open ocean oligotrophic ecosystems where dissolved nutrients are scarce and often limit biological productivity. It has previously been proposed that O. universa meets its nitrogen (N) requirements by preying on zooplankton, and that its symbiotic dinoflagellates recycle metabolic 'waste ammonium' for their N pool. However, these conclusions were derived from bulk 15N-enrichment experiments and model calculations, and our understanding of N assimilation and exchange between the foraminifer host cell and its symbiotic dinoflagellates remains poorly constrained. Here, we present data from pulse-chase experiments with 13C-enriched inorganic carbon, 15N-nitrate, and 15N-ammonium, as well as a 13C- and 15N- enriched heterotrophic food source, followed by TEM (transmission electron microscopy) coupled to NanoSIMS (nanoscale secondary ion mass spectrometry) isotopic imaging to visualize and quantify C and N assimilation and translocation in the symbiotic system. High levels of 15N-labelling were observed in the dinoflagellates and in foraminiferal organelles and cytoplasm after incubation with 15N-ammonium, indicating efficient ammonium assimilation. Only weak 15N-assimilation was observed after incubation with 15N-nitrate. Feeding foraminifers with 13C- and 15N-labelled food resulted in dinoflagellates that were labelled with 15N, thereby confirming the transfer of 15N-compounds from the digestive vacuoles of the foraminifer to the symbiotic dinoflagellates, likely through recycling of ammonium. These observations are important for N isotope-based palaeoceanographic reconstructions, as they show that δ15N values recorded in the organic matrix in symbiotic species likely reflect ammonium recycling rather than alternative N sources, such as nitrates.

LeKieffre|Charlotte|C|
Spero|Howard J|HJ|
Fehrenbacher|Jennifer S|JS|
Russell|Ann D|AD|
Ren|Haojia|H|
Geslin|Emmanuelle|E|
Meibom|Anders|A|

planktonic foraminifera
ammonium assimilation
ammonium recycling photosymbiosis
dinoflagellate symbiosis


Factors involved in the etiology of temporomandibular disorders - a literature review.

Abstract:
BACKGROUND AND AIM
This review aims at presenting a current view on the most frequent factors involved in the mechanisms causing temporomandibular disorders (TMD).

METHOD
We conducted a critical review of the literature for the period January 2000 to December 2014 to identify factors related to TMD development and persistence.

RESULTS
The etiology of TMD is multidimensional: biomechanical, neuromuscular, bio-psychosocial and biological factors may contribute to the disorder. Occlusal overloading and parafunctions (bruxism) are frequently involved as biomechanical factors; increased levels of estrogen hormones are considered biological factors affecting the temporo-mandibular-joint. Among bio-psychosocial factors, stress, anxiety or depression, were frequently encountered.

CONCLUSIONS
The etiopathogenesis of this condition is poorly understood, therefore TMDs are difficult to diagnose and manage. Early and correct identification of the possible etiologic factors will enable the appropriate treatment scheme application in order to reduce or eliminate TMDs debilitating signs and symptoms.

Chisnoiu|Andrea Maria|AM|
Picos|Alina Monica|AM|
Popa|Sever|S|
Chisnoiu|Petre Daniel|PD|
Lascu|Liana|L|
Picos|Andrei|A|
Chisnoiu|Radu|R|

factors
etiology
pathogenesis
temporomandibular joint disorder


Quality of osmotically pre-treated and vacuum dried pineapple cubes on storage as influenced by type of solutes and packaging materials.

Abstract: The quality and stability of osmotically pre-treated and subsequently vacuum dried pineapple cubes using three different solutes and packed in three different types of packaging materials on storage was evaluated. The experiment was laid out in completely randomized block design with two factors and three replications for each treatment. Treatment combinations were considered as one factor and storage interval as another factor. Pineapple cubes stored in glass bottle showed very little percentage variation in moisture content due to its high moisture barrier properties. In all treatment combination, acidity values were invariably found to increase as the storage progressed. For all three different osmotic treatments, HDPE pouch packet always showed highest acidity followed by PVDC pouch. Again among three solutes under consideration, invert sugar recorded a rapid increase in acidity than other solutes. In pineapple cubes osmotically treated with sucrose solution, the rates of decrease of total sugar content were lower than that of invert sugar and sorbitol treated pineapple cubes. The percentage decrease of total sugar content was highest when the osmotically dehydrated pineapple cubes were packed in HDPE pouch and it was least in glass bottles. There was a gradual decrease in ascorbic acid content with the extension of storage period and this decrease was statistically significant at all storage intervals up to six-month. Lowest value of ascorbic acid content (15.210 mg per 100 g initial solid) was recorded in invert sugar treated pineapple cube packed in HDPE pouch after 6 months of storage.

Paul|Prodyut Kumar|PK|
Ghosh|Swapan Kumar|SK|
Singh|Dhananjay Kumar|DK|
Bhowmick|Nilesh|N|

Pineapple
Storage
Invert sugar
Sucrose
Sorbitol
Osmotic dehydration


Limits of the Effective Medium Theory in Particle Amplified Surface Plasmon Resonance Spectroscopy Biosensors.

Abstract: The resonant wave modes in monomodal and multimodal planar Surface Plasmon Resonance (SPR) sensors and their response to a bidimensional array of gold nanoparticles (AuNPs) are analyzed both theoretically and experimentally, to investigate the parameters that rule the correct nanoparticle counting in the emerging metal nanoparticle-amplified surface plasmon resonance (PA-SPR) spectroscopy. With numerical simulations based on the Finite Element Method (FEM), we evaluate the error performed in the determination of the surface density of nanoparticles σ when the Maxwell-Garnett effective medium theory is used for fast data processing of the SPR reflectivity curves upon nanoparticle detection. The deviation increases directly with the manifestations of non-negligible scattering cross-section of the single nanoparticle, dipole-dipole interactions between adjacent AuNPs and dipolar interactions with the metal substrate. Near field simulations show clearly the set-up of dipolar interactions when the dielectric thickness is smaller than 10 nm and confirm that the anomalous dispersion usually observed experimentally is due to the failure of the effective medium theories. Using citrate stabilized AuNPs with a nominal diameter of about 15 nm, we demonstrate experimentally that Dielectric Loaded Waveguides (DLWGs) can be used as accurate nanocounters in the range of surface density between 20 and 200 NP/µm², opening the way to the use of PA-SPR spectroscopy on systems mimicking the physiological cell membranes on SiO₂ supports.

Costa|Jefferson S|JS|
Zaman|Quaid|Q|0000-0002-9274-1919
Q da Costa|Karlo|K|0000-0003-4393-3217
Dmitriev|Victor|V|
Pandoli|Omar|O|0000-0002-2220-7817
Fontes|Giselle|G|
Del Rosso|Tommaso|T|0000-0002-0600-4139

D015374:Biosensing Techniques
D019343:Citric Acid
D020342:Finite Element Analysis
D006046:Gold
D053768:Metal Nanoparticles
D010316:Particle Size
D012822:Silicon Dioxide
D020349:Surface Plasmon Resonance

Particle Amplified Surface Plasmon Resonance Spectroscopy (PA-SPR)


Characteristics of retinal vein occlusion with final vision better than 78 letters after sequential therapy with ranibizumab and triamcinolone acetate.

Abstract:
AIM
To analyze the reasons that may lead to the different vision result by combining the ranibizumab and triamcinolone acetate (TA) in sequence to treat macular edema in retinal vein occlusion (RVO).

METHODS
Ranibizumab and TA were combined in sequence to treat 43 patients with macular edema secondary to RVO. Six months after the treatment, patients with central fovea thickness (CFT) less than 300 µm in optical coherence tomography (OCT) were collected into Groups I and II, based on vision acuity (VA) better than 78 letters or less than 60 letters. The age, baseline VA, duration from onset to treatment, CFT at the baseline, sub-retinal fluid (SRF), sub-foveal exudates and injection times of TA and ranibizumab were taken into comparison.

RESULTS
The mean age of the subjects was 46.4y in Group I but 57.5y in Group II. The difference of age was significant between groups (P<0.01). The mean baseline VA was 51.4 letters in Group I and 43.9 letters in Group II (P<0.05). The baseline CFT were 670.9 µm in Group I with SRF in 54.3% patients and 678.1 µm in Group II with SRF in 52.9% (P>0.05). The mean number of injections of TA was 0.9 and the mean number of injections of ranibizumab was 2.3 in Group I but 1.7 and 2.9 respectively in Group II. The treatment times of ranibizumab had no difference between the 2 groups (P>0.05) but the difference of TA injection times was significant, P<0.05. Subfoveal exudates at final stage happened in no subjects in Group I but in 45.83% subjects in Group II.

CONCLUSION
This combined treatment is safer than TA injection and cheaper than ranibizumab injection alone. Younger patients and earlier treatment will help to get better vision outcome. Subfoveal exudates at the final stage have significant relationship with vision outcome. No relationship existed between the baseline CFT, SRF and the vision outcome.

retinal vein occlusion
sequential therapy
ranibizumab
triamcinolone acetate
macular edema


Making Headway for Discussions About Concussions: Experiences of Former High School and Collegiate Student-Athletes.

Abstract: In order to better understand how to identify and treat student-athletes who experience concussions, better insight into reporting behavior of athletes is needed. This study aims to identify information influencing athletes' attitudes toward reporting their concussions and the perceived trajectory of their recovery both athletically and academically. Twenty-five former high school and collegiate athletes who experienced concussions in a wide variety of sports, organizational levels, and time periods gave insight through structured, qualitative interviews. A number of factors regarding education about concussions, proper diagnosis of concussions, and motivational pressures within high school and collegiate athletics were assessed. Eight major themes were identified regarding the participants' experiences with sport-related concussion: optimism bias, invisibility of the injury, diagnostic barriers, desire to play, external support and pressures, uncertainty of long-term prognosis, generational factors, and protection of future athletes. The findings support that underreporting of concussions among those players interviewed is related to misperceived risk, lack of education, and a struggle between internal and external pressures to play through injury. However, those who did seek medical and academic support, often did receive the necessary aid.

Oddo|Anthony|A|
O'Conor|Ellen|E|
Shore|Sarah|S|
Piraino|Mary|M|
Gibney|Kyla|K|
Tsao|Jack|J|
Stanfill|Ansley Grimes|AG|

concussion
athlete
sports
adolescent
traumatic brain injury


Effects of 1,25(OH)2D3 on proliferation and apoptosis of human glomerular mesangial cells.

Abstract:
OBJECTIVE
To investigate the effect of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] on cell proliferation and apoptosis of human glomerular mesangial cells.

METHODS
Human glomerular mesangial cells were cultured for 48 h and randomly divided into four groups, normal control group (N group), proliferation group (EGF group ), vitamin D3 intervention group (VD3 group), proliferation intervention group (EGF+VD3 group ). Flow cytometry was used to detect the cell cycle and apoptosis rate, and Western blot was used to detect the PCNA and Caspases-3 expression. The effect of 1,25(OH)2D3 on human mesangial cell proliferation was detected by CCK-8.

RESULTS
Compared with the control group, G1 phase cells in EGF group were significantly reduced, S, G2/M phase cells were increased, and the higher PI and PCNA expression levels were increased; G1 phase cells in VD3 group were increased, S, G2/M phase cells were decreased, and the lower PI and PCNA expression levels were reduced. Compared with EGF group, G1 phase cells in EGF+VD3 group were increased, S, G2/M phase cells were decreased, and the lower PI and PCNA expression levels were significantly reduced. Compared with normal control group, the apoptosis rate in EGF group was significantly lower, and the Caspase-3 expression level was reduced; the apoptosis rate in VD3 group was significantly higher, and Caspase-3 expression was significantly increased. Compared with EGF group, the apoptotic rate of mesangial cells in EGF+VD3 group was significantly increased, and the Caspase-3 expression level was increased, with significant difference.

CONCLUSION
1,25(OH)2D3 inhibited the proliferation of mesangial cells and induced their apoptosis by blocking the cell cycle, inhibiting expression of PCNA, and upregulating Caspase-3 expression.

Zhang|Chun-Jiang|CJ| Zhao|Dan|D| Yin|Xuan|X| Zhang|Hao|H| Ma|Li|L| Chen|Jian-Ping|JP| Liu|Chun|C| Yang|Xiao-Ping|XP|

proliferating cell nuclear antigen (PCNA)


The marine bacterium Marinobacter hydrocarbonoclasticus SP17 degrades a wide range of lipids and hydrocarbons through the formation of oleolytic biofilms with distinct gene expression profiles.

Abstract: Hydrophobic organic compounds (mainly lipids and hydrocarbons) represent a significant part of the organic matter in marine waters, and their degradation has an important impact in the carbon fluxes within oceans. However, because they are nearly insoluble in the water phase, their degradation by microorganisms occurs at the interface with water and thus requires specific adaptations such as biofilm formation. We show that Marinobacter hydrocarbonoclasticus SP17 develops biofilms, referred to as oleolytic biofilms, on a large variety of hydrophobic substrates, including hydrocarbons, fatty alcohols, fatty acids, triglycerides, and wax esters. Microarray analysis revealed that biofilm growth on n-hexadecane or triolein involved distinct genetic responses, together with a core of common genes that might concern general mechanisms of biofilm formation. Biofilm growth on triolein modulated the expression of hundreds of genes in comparison with n-hexadecane. The processes related to primary metabolism and genetic information processing were downregulated. Most of the genes that were overexpressed on triolein had unknown functions. Surprisingly, their genome localization was restricted to a few regions identified as putative genomic islands or mobile elements. These results are discussed with regard to the adaptive responses triggered by M. hydrocarbonoclasticus SP17 to occupy a specific niche in marine ecosystems.

Mounier|Julie|J| Camus|Arantxa|A| Mitteau|Isabelle|I| Vaysse|Pierre-Joseph|PJ| Goulas|Philippe|P| Grimaud|Régis|R| Sivadon|Pierre|P|

D057927:Hydrophobic and Hydrophilic Interactions

degradation of hydrophobic organic carbon


Quercetin and the ocular surface: What we know and where we are going.

Abstract: Flavonoids are a class of plant and fungus secondary metabolites that serve functional roles in protecting against UV-induced oxidative stress, mediating auxin signaling, and promoting microbial defense. Flavonoids are extremely abundant in nature where their potent antioxidant capacity and very low toxicity makes them highly attractive as potential therapeutic agents. In terms of clinical applications, neither the Food and Drug Administration (FDA) nor the European Food Safety Authority (EFSA) has approved any health claims or drugs related to the use of flavonoids for therapeutic purposes. Quercetin is a common flavonol that has been shown to have potent antioxidant, anti-inflammatory, and anti-fibrotic activities both in vitro and in vivo in various tissues. Recently, the application of quercetin as a therapeutic has been gaining attention in the ocular surface scientific community in the study of dry eye, keratoconus, inflammation, and neovascularization of the cornea. This review will discuss the latest findings and the use of quercetin for the treatment of dystrophies of the ocular surface. Impact statement The eye represents a small portion of the human body, accounting for one decimal fraction of the anterior body surface. The cornea is an avascular, transparent tissue that acts as a primary barrier against mechanical and infectious damaging agents, protecting the internal structures of the eye. Corneal survival and function are affected by a number of factors including but not limited to injury, trauma, infection, genetics, and environment. Corneal injury, or trauma, often leads to loss of corneal transparency and even blindness. The concept of "curing" corneal opacity has been discussed in published form for over 200 years. Currently, full corneal transplant is the only treatment option. There is a strong interest in developing natural therapeutic products that come with minimum side effects. A novel antioxidant flavonoid, quercetin, has been gaining traction as a potential therapeutic to prevent the injured cornea. This review discusses the potential of this antioxidant.

Quercetin
ocular surface
flavonoids
anti-inflammatory
anti-fibrotic
cornea
wound healing


The robust identification of exchange from T2-T2 time-domain features.

Abstract: Two-dimensional spin-spin relaxation (T2-T2) techniques have been developed to probe coupling between different environments such as diffusive coupling between small and large pores or chemical exchange with clays. In these studies, Numerical Laplace Inversion (NLI) is used to obtain two-dimensional T2-T2 relaxation distribution spectrum from the T2-T2 signal decays, and the off-diagonal peaks characterize coupling. Often, these coupling peaks are both weak and close to the diagonal and combined with the inherently ill-conditioned nature of the inversion, their presence is difficult to differentiate from inversion related artifacts and blurring. This manuscript presents a time domain based analysis to identify the presence of coupling that avoids the ambiguities present in T2-T2 spectra. The approach utilizes the symmetric nature of the two-dimensional time domain data, where the presence of curvature along one of these symmetries gives an unambiguous indicator of coupling. Measurements on porous glass beads are used to verify the technique.

Song|Ruobing|R|
Song|Yi-Qiao|YQ|
Vembusubramanian|Muthusamy|M|
Paulsen|Jeffrey L|JL|

CPMG
Inverse laplace
Multi-dimensional relaxometry
Porous media
T(2)–T(2) exchange
TD-NMR
Time-domain analysis


Smoking Cessation during the Second Half of Pregnancy Prevents Low Birth Weight among Australian Born Babies in Regional New South Wales.

Abstract: Smoking during pregnancy is a modifiable risk behavior of adverse health outcomes including low birth weight (LBW), and LBW is a key marker of newborns immediate and future health. This study aimed to examine the association between smoking cessation during the second half of pregnancy and LBW among babies born in Southern New South Wales Local Health District (SNSWLHD). Routinely collected perinatal data on singleton live births for the period 2011-2019 in five public hospitals of SNSWLHD were utilized. Multivariate logistic regression models were fitted to examine the association between smoking cessation during the second half of pregnancy and LBW. Analyses showed that mothers who ceased smoking during the second half of pregnancy were 44% less likely to have LBW babies (adjusted odds ratio (aOR) = 0.56; 95% confidence interval (CI): 0.34, 0.94) compared to those who continued smoking throughout pregnancy. Mothers who reported an average daily dose of 1-10 or >10 cigarettes during the second half of pregnancy were significantly more likely to have babies with LBW than those who ceased smoking during the second half of pregnancy. Early identification of smoking behavior and promotion of smoking-cessation intervention for risk populations including pregnant women within the older age bracket (35-49 years) is imperative to reduce LBW.

smoking cessation
pregnancy
low birth weight
Australia
epidemiology
tobacco smoking


Insights into the function of the phasiRNA-triggering miR1514 in response to stress in legumes.

Abstract: We recently described the activity of miR1514a in response to water deficit in Phaseolus vulgaris. Pvu-miR1514a targets a NAC transcription factor mRNA for cleavage and subsequently triggers NAC-derived phasiRNA formation. Here we show that accumulation and activity of miR1514a are also conserved in the model legume Medicago truncatula. Consistently, we identified Mtr-miR1514a and detected its increased accumulation in response to stress conditions, targeting a NAC TF mRNA for cleavage and triggering phasiRNA production. In P. vulgaris, miR1514a inhibition in transgenic hairy roots was reported to increase NAC 700 mRNA levels and to affect expression patterns of several genes, including that of a Sec 14 homolog. We report here that in adult plant roots exposed to dehydration conditions, where miR1514a levels increased and NAC 700 mRNA decreased, there was a reduction of Sec 14 homolog mRNA levels, suggesting a direct transcriptional effect. The functions of miR1514a, NAC 700 and derived phasiRNAs have just begun to be elucidated in common bean; future understanding of their activities in this and other legumes species will advance our knowledge of microRNA functions in plants.

Sosa-Valencia|Guadalupe|G|
Romero-Pérez|Paulette S|PS|
Palomar|V Miguel|VM|
Covarrubias|Alejandra A|AA|
Reyes|José L|JL|

D018506:Gene Expression Regulation, Plant

Phaseolus vulgaris
Medicago truncatula
Dehydration
microRNAs
Glycine max
Sec 14-like
phased siRNAs


Mechanisms of spreading depolarization in vertebrate and insect central nervous systems.

Abstract: Spreading depolarization (SD) is generated in the central nervous systems of both vertebrates and invertebrates. SD manifests as a propagating wave of electrical depression caused by a massive redistribution of ions. Mammalian SD underlies a continuum of human pathologies from migraine to stroke damage, whereas insect SD is associated with environmental stress-induced neural shutdown. The general cellular mechanisms underlying SD seem to be evolutionarily conserved throughout the animal kingdom. In particular, SD in the central nervous system of Locusta migratoria and Drosophila melanogaster has all the hallmarks of mammalian SD. Locust SD is easily induced and monitored within the metathoracic ganglion (MTG) and can be modulated both pharmacologically and by preconditioning treatments. The finding that the fly brain supports repetitive waves of SD is relatively recent but noteworthy, since it provides a genetically tractable model system. Due to the human suffering caused by SD manifestations, elucidating control mechanisms that could ultimately attenuate brain susceptibility is essential. Here we review mechanisms of SD focusing on the similarities between mammalian and insect systems. Additionally we discuss advantages of using invertebrate model systems and propose insect SD as a valuable model for providing new insights to mammalian SD.


Effects of gamma radiation on microbial, physicochemical, and structural properties of whey protein model system.

Abstract: Gamma radiation has been used in food processing for many years, though it has certain effects on food components. Whey protein solutions (10%/30%, wt/vol) were treated with gamma radiation at various dosages (10-25 kGy) and evaluated for microbial changes in the solutions and physicochemical and structural changes of whey proteins. Whey protein solutions after gamma radiation showed substantially lower populations of all viable microorganisms than those of controls. The 10% whey protein solution treated at radiation of 20 or 25 kGy remained sterile for up to 4 wk at room temperature. Gamma radiation increased viscosity and turbidity and decreased soluble nitrogen of whey protein solutions compared to nonradiated control samples regardless of radiation dosage. Nonreducing sodium dodecyl sulfate-PAGE suggested that whey proteins under gamma radiation treatment formed aggregates with high molecular weights. Reducing sodium dodecyl sulfate-PAGE showed that disulfide bonds played a role in gamma radiation-induced whey protein cross-linking. Scanning and transmission electron microscopy micrographs exhibited large aggregates of whey proteins after gamma radiation treatment. Results suggested that gamma radiation could be applied to whey protein solution for purposes of reducing microbial counts and cross-linking protein molecules.

Wang|X B|XB|
Wang|C N|CN|
Zhang|Y C|YC|
Liu|T T|TT|
Lv|J P|JP|
Shen|X|X|
Guo|M R|MR|

gamma radiation
structural property
whey protein
physicochemical property


Meta-Review of CSF Core Biomarkers in Alzheimer's Disease: The State-of-the-Art after the New Revised Diagnostic Criteria.

Abstract:
BACKGROUND
Current research criteria for Alzheimer's disease (AD) include cerebrospinal fluid (CSF) biomarkers into the diagnostic algorithm. However, spreading their use to the clinical routine is still questionable.

OBJECTIVE
To provide an updated, systematic and critical review on the diagnostic utility of the CSF core biomarkers for AD.

DATA SOURCES
MEDLINE, PreMedline, EMBASE, PsycInfo, CINAHL, Cochrane Library, and CRD.

ELIGIBILITY CRITERIA
(1a) Systematic reviews with meta-analysis; (1b) Primary studies published after the new revised diagnostic criteria; (2) Evaluation of the diagnostic performance of at least one CSF core biomarker.

RESULTS
The diagnostic performance of CSF biomarkers is generally satisfactory. They are optimal for discriminating AD patients from healthy controls. Their combination may also be suitable for mild cognitive impairment (MCI) prognosis. However, CSF biomarkers fail to distinguish AD from other forms of dementia.

LIMITATIONS
(1) Use of clinical diagnosis as standard instead of pathological postmortem confirmation; (2) variability of methodological aspects; (3) insufficiently long follow-up periods in MCI studies; and (4) lower diagnostic accuracy in primary care compared with memory clinics.

CONCLUSION
Additional work needs to be done to validate the application of CSF core biomarkers as they are proposed in the new revised diagnostic criteria. The use of CSF core biomarkers in clinical routine is more likely if these limitations are overcome. Early diagnosis is going to be of utmost importance when effective pharmacological treatment will be available and the CSF core biomarkers can also be implemented in clinical trials for drug development.

Ferreira|Daniel|D| Perestelo-Pérez|Lilisbeth|L| Westman|Eric|E| Wahlund|Lars-Olof|LO| Sarría|Antonio|A| Serrano-Aguilar|Pedro|P|

meta-review
Alzheimer’s disease
amyloid beta-protein (42)
cerebrospinal fluid biomarkers
sensitivity
specificity
state-of-the-art review
tau protein


DMSO- and Serum-Free Cryopreservation of Wharton's Jelly Tissue Isolated From Human Umbilical Cord.

Abstract: The facile nature of mesenchymal stem cell (MSC) acquisition in relatively large numbers has made Wharton's jelly (WJ) tissue an alternative source of MSCs for regenerative medicine. However, freezing of such tissue using dimethyl sulfoxide (DMSO) for future use impedes its clinical utility. In this study, we compared the effect of two different cryoprotectants (DMSO and cocktail solution) on post-thaw cell behavior upon freezing of WJ tissue following two different freezing protocols (Conventional [-1°C/min] and programmed). The programmed method showed higher cell survival rate compared to conventional method of freezing. Further, cocktail solution showed better cryoprotection than DMSO. Post-thaw growth characteristics and stem cell behavior of Wharton's jelly mesenchymal stem cells (WJMSCs) from WJ tissue cryopreserved with a cocktail solution in conjunction with programmed method (Prog-Cock) were comparable with WJMSCs from fresh WJ tissue. They preserved their expression of surface markers, pluripotent factors, and successfully differentiated in vitro into osteocytes, adipocytes, chondrocytes, and hepatocytes. They also produced lesser annexin-V-positive cells compared to cells from WJ tissue stored using cocktail solution in conjunction with the conventional method (Conv-Cock). Real-time PCR and Western blot analysis of post-thaw WJMSCs from Conv-Cock group showed significantly increased expression of pro-apoptotic factors (BAX, p53, and p21) and reduced expression of anti-apoptotic factor (BCL2) compared to WJMSCs from the fresh and Prog-Cock group. Therefore, we conclude that freezing of fresh WJ tissue using cocktail solution in conjunction with programmed freezing method allows for an efficient WJ tissue banking for future MSC-based regenerative therapies. J. Cell. Biochem. 117: 2397-2412, 2016. © 2016 Wiley Periodicals, Inc.

Shivakumar|Sharath Belame|SB|
Bharti|Dinesh|D|
Subbarao|Raghavendra Baregundi|RB|
Jang|Si-Jung|SJ|
Park|Ji-Sung|JS|
Ullah|Imran|I|
Park|Ji-Kwon|JK|
Byun|June-Ho|JH|
Park|Bong-Wook|BW|
Rho|Gyu-Jin|GJ|

D060888:Real-Time Polymerase Chain Reaction

D020133:Reverse Transcriptase Polymerase Chain Reaction

WHARTON'S JELLY TISSUE
FREEZING
CRYOPROTECTANTS
COCKTAIL SOLUTION
CELL SURVIVAL RATE
WHARTON'S JELLY MESENCHYMAL STEM CELLS


Encephalitozoon cuniculi and Vittaforma corneae (Phylum Microsporidia) inhibit staurosporine-induced apoptosis in human THP-1 macrophages in vitro.

Abstract: Obligately intracellular microsporidia regulate their host cell life cycles, including apoptosis, but this has not been evaluated in phagocytic host cells such as macrophages that can facilitate infection but also can be activated to kill microsporidia. We examined two biologically dissimilar human-infecting microsporidia species, Encephalitozoon cuniculi and Vittaforma corneae, for their effects on staurosporine-induced apoptosis in the human macrophage-differentiated cell line, THP1. Apoptosis was measured after exposure of THP-1 cells to live and dead mature organisms via direct fluorometric measurement of Caspase 3, colorimetric and fluorometric TUNEL assays, and mRNA gene expression profiles using Apoptosis RT2 Profiler PCR Array. Both species of microsporidia modulated the intrinsic apoptosis pathway. In particular, live E. cuniculi spores inhibited staurosporine-induced apoptosis as well as suppressed pro-apoptosis genes and upregulated anti-apoptosis genes more broadly than V. corneae. Exposure to dead spores induced an opposite effect. Vittaforma corneae, however, also induced inflammasome activation via Caspases 1 and 4. Of the 84 apoptosis-related genes assayed, 42 (i.e. 23 pro-apoptosis, nine anti-apoptosis, and 10 regulatory) genes were more affected including those encoding members of the Bcl2 family, caspases and their regulators, and members of the tumour necrosis factor (TNF)/TNF receptor R superfamily.

Caspases
TUNEL
immune evasion
inflammation
intracellular parasites
opportunistic parasites


Effects of Timely Control Intervention on the Spread of Middle East Respiratory Syndrome Coronavirus Infection.

Abstract:
Objectives
The 2015 Middle East Respiratory Syndrome Coronavirus (MERS-CoV) outbreak in Korea caused major economic and social problems. The control intervention was conducted during the MERS-CoV outbreak in Korea immediately after the confirmation of the index case. This study investigates whether the early risk communication with the general public and mass media is an effective preventive strategy.

Methods
The SEIR (Susceptible, Exposed, Infectious, Recovered) model with estimated parameters for the time series data of the daily MERS-CoV incidence in Korea was considered from May to December 2015. For 10,000 stochastic simulations, the SEIR model was computed using the Gillespie algorithm. Depending on the time of control intervention on the 20th, 40th, and 60th days after the identification of the index case, the box plots of MERS-CoV incidences in Korea were computed, and the results were analyzed via ANOVA.

Results
The box plots showed that there was a significant difference between the non-intervention and intervention groups (the 20th day, 40th day, and 60th day groups) and seemed to show no significant difference based on the time of intervention. However, the ANOVA revealed that early intervention was a good strategy to control the disease.

Conclusion
Appropriate risk communication can secure the confidence of the general public in the public health authorities.

Choi|Ilsu|I|
Lee|Dong Ho|DH|
Kim|Yongkuk|Y|

Middle East respiratory syndrome coronavirus


Novel TRAIL sensitizer Taraxacum officinale F.H. Wigg enhances TRAIL-induced apoptosis in Huh7 cells.

Abstract: TRAIL (TNF-related apoptosis inducing ligand) is a promising anti-cancer drug target that selectively induces apoptosis in cancer cells. However, many cancer cells are resistant to TRAIL-induced apoptosis. Therefore, reversing TRAIL resistance is an important step for the development of effective TRAIL-based anti-cancer therapies. We previously reported that knockdown of the TOR signaling pathway regulator-like (TIPRL) protein caused TRAIL-induced apoptosis by activation of the MKK7-c-Jun N-terminal Kinase (JNK) pathway through disruption of the MKK7-TIPRL interaction. Here, we identified Taraxacum officinale F.H. Wigg (TO) as a novel TRAIL sensitizer from a set of 500 natural products using an ELISA system and validated its activity by GST pull-down analysis. Furthermore, combination treatment of Huh7 cells with TRAIL and TO resulted in TRAIL-induced apoptosis mediated through inhibition of the MKK7-TIPRL interaction and subsequent activation of MKK7-JNK phosphorylation. Interestingly, HPLC analysis identified chicoric acid as a major component of the TO extract, and combination treatment with chicoric acid and TRAIL induced TRAIL-induced cell apoptosis via JNK activation due to inhibition of the MKK7-TIPRL interaction. Our results suggest that TO plays an important role in TRAIL-induced apoptosis, and further functional studies are warranted to confirm the importance of TO as a novel TRAIL sensitizer for cancer therapy. © 2015 Wiley Periodicals, Inc.

Yoon|Ji-Yong|JY|
Cho|Hyun-Soo|HS|
Lee|Jeong-Ju|JJ|
Lee|Hyo-Jung|HJ|
Jun|Soo Young|SY|
Lee|Jae-Hye|JH|
Song|Hyuk-Hwan|HH|
Choi|SangHo|S|
Saloura|Vassiliki|V|
Park|Choon Gil|CG|
Kim|Cheol-Hee|CH|
Kim|Nam-Soon|NS|

D047908:Intracellular Signaling Peptides and Proteins

D048031:JNK Mitogen-Activated Protein Kinases

Taraxacum officinale F.H. Wigg
apoptosis
TRAIL resistance
TIPRL
HCC


Developmental trajectories of adaptive behavior in autism spectrum disorder: a high-risk sibling cohort.

Abstract:
BACKGROUND
Children with autism spectrum disorder (ASD) often experience impairments in adaptive behavior.

METHODS
Developmental trajectories of adaptive behavior in ASD were examined in children from high-risk (siblings of children diagnosed with ASD, n = 403) and low-risk (no family history of ASD, n = 163) families. Children were assessed prospectively at 12, 18, 24, and 36 months of age using the Vineland Adaptive Behavior Scales and underwent a blind independent diagnostic assessment for ASD at 36 months of age.

RESULTS
The semi-parametric group-based modeling approach using standard scores on the Adaptive Behavior Composite revealed three distinct developmental trajectories: (a) Group 1 (21.2% of sample) showed average performance at 12 months and a declining trajectory; (b) Group 2 (52.8% of the sample) showed average performance at 12 months with a slightly declining trajectory; and (c) Group 3 (26.0% of the sample) showed a higher level of adaptive behavior at 12 months and a stable trajectory. The Mullen Scales of Early Learning Early Learning Composite and the Autism Observation Scale for Infants total score at 6 and 12 months predicted trajectory membership.

CONCLUSIONS
The results emphasize heterogeneous development associated with ASD and the need for interventions tailored to individual presentations.

Sacrey|Lori-Ann R|LR|
Zwaigenbaum|Lonnie|L|
Bryson|Susan|S|
Brian|Jessica|J|
Smith|Isabel M|IM|
Raza|Sarah|S|
Roberts|Wendy|W|
Szatmari|Peter|P|
Vaillancourt|Tracy|T|
Roncadin|Caroline|C|
Garon|Nancy|N|

Autism spectrum disorders
development
adaptive behaviour
high risk studies


Four-Dimensional Ultrafast Electron Microscopy: Insights into an Emerging Technique.

Abstract: Four-dimensional ultrafast electron microscopy (4D-UEM) is a novel analytical technique that aims to fulfill the long-held dream of researchers to investigate materials at extremely short spatial and temporal resolutions by integrating the excellent spatial resolution of electron microscopes with the temporal resolution of ultrafast femtosecond laser-based spectroscopy. The ingenious use of pulsed photoelectrons to probe surfaces and volumes of materials enables time-resolved snapshots of the dynamics to be captured in a way hitherto impossible by other conventional techniques. The flexibility of 4D-UEM lies in the fact that it can be used in both the scanning (S-UEM) and transmission (UEM) modes depending upon the type of electron microscope involved. While UEM can be employed to monitor elementary structural changes and phase transitions in samples using real-space mapping, diffraction, electron energy-loss spectroscopy, and tomography, S-UEM is well suited to map ultrafast dynamical events on materials surfaces in space and time. This review provides an overview of the unique features that distinguish these techniques and also illustrates the applications of both S-UEM and UEM to a multitude of problems relevant to materials science and chemistry.

4D ultrafast electron microscopy
atomic resolution
charge carrier dynamics
electron impact dynamics
energy loss mechanism
surface dynamics


Comparison of prefrontal hemodynamic responses and cognitive deficits between adult patients with autism spectrum disorder and schizophrenia.

Abstract: Autism spectrum disorder (ASD) and schizophrenia share many phenotypic characteristics, but their association with prefrontal function have not been directly compared. The aim of this study is to compare cognitive profiles and their association with the prefrontal function between the two groups. We explored prefrontal dysfunction among adult individuals with ASD (n = 32), schizophrenia (n = 87), and healthy controls (HCs; n = 50). We assessed cognitive function in all participants using the Brief Assessment of Cognition in Schizophrenia (BACS). The BACS data of patients with schizophrenia were entered into hierarchical cluster analyses to assign subjects to a specific subgroup based on individual profiles. Using near-infrared spectroscopy, we measured hemodynamic responses in the fronto-temporal regions during a working memory task. Among the patients with schizophrenia, we defined 4 neurocognitive subgroups, including a global impairment, a mild impairment, and 2 selective impairment groups. Compared to the HCs, the ASD and schizophrenia groups had much weaker hemodynamic responses in the left DLPFC, left frontopolar cortex (FPC), and left inferior frontal gyrus. The ASD group showed a similar level of cognitive impairment with the mild level subgroup of schizophrenia. Additionally, the two groups shared reduced activity in the left DLPFC and left FPC during the task compared to HCs. Moreover, the BACS composite scores correlated positively with hemodynamic responses in a broad area involving fronto-temporal regions in the total patient sample. This research indicates considerable similarity in the left PFC dysfunction and its association with cognitive deficits between the disorders. These findings may guide future studies that investigate pathophysiological similarities between ASD and schizophrenia.

Pu|Shenghong|S| Nakagome|Kazuyuki|K| Satake|Takahiro|T| Ohtachi|Hiroaki|H| Itakura|Masashi|M| Yamanashi|Takehiko|T| Miura|Akihiko|A| Yokoyama|Katsutoshi|K| Matsumura|Hiroshi|H| Iwata|Masaaki|M| Nagata|Izumi|I| Kaneko|Koichi|K|

D011569:Psychiatric Status Rating Scales


Cysteine-Induced Hybridization of 2D Molybdenum Disulfide Films for Efficient and Stable Hydrogen Evolution Reaction.

Abstract: The noble, metal-free materials capable of efficiently catalyzing water splitting reactions currently hold a great deal of promise. In this study, we reported the structure and electrochemical performance of new MoS2-based material synthesized with L-cysteine. For this, a facile one-pot hydrothermal process was developed and an array of densely packed nanoplatelet-shaped hybrid species directly on a conductive substrate were obtained. The crucial role of L-cysteine was determined by numerous methods on the structure and composition of the synthesized material and its activity and stability for hydrogen evolution reaction (HER) from the acidic water. A low Tafel slope of 32.6 mV dec-1, close to a Pt cathode, was registered for the first time. The unique HER performance at the surface of this hybrid material in comparison with recently reported MoS2-based electrocatalysts was attributed to the formation of more defective 1T, 2H-MoS2/MoOx, C nanostructures with the dominant 1T-MoS2 phase and thermally degraded cysteine residues entrapped. Numerous stacks of metallic (1T-MoS2 and MoO2) and semiconducting (2H-MoS2 and MoO3) fragments relayed the formation of highly active layered nanosheets possessing a low hydrogen adsorption free energy and much greater durability, whereas intercalated cysteine fragments had a low Tafel slope of the HER reaction. X-ray photoelectron spectroscopy, scanning electron microscopy, thermography with mass spectrometry, high-resolution transmission electron microscopy, Raman spectroscopy techniques, and linear sweep voltammetry were applied to verify our findings.

molybdenum disulfide
water splitting
L-cysteine
electrocatalyst
hybrid films
hydrothermal synthesis


Dipteran larvae and microbes facilitate nutrient sequestration in the Nepenthes gracilis pitcher plant host.

Abstract: The fluid-containing traps of Nepenthes carnivorous pitcher plants (Nepenthaceae) are often inhabited by organisms known as inquilines. Dipteran larvae are key components of such communities and are thought to facilitate pitcher nitrogen sequestration by converting prey protein into inorganic nitrogen, although this has never been demonstrated in Nepenthes Pitcher fluids are also inhabited by microbes, although the relationship(s) between these and the plant is still unclear. In this study, we examined the hypothesis of digestive mutualism between N. gracilis pitchers and both dipteran larvae and fluid microbes. Using dipteran larvae, prey and fluid volumes mimicking in situ pitcher conditions, we conducted in vitro experiments and measured changes in available fluid nitrogen in response to dipteran larvae and microbe presence. We showed that the presence of dipteran larvae resulted in significantly higher and faster releases of ammonium and soluble protein into fluids in artificial pitchers, and that the presence of fluid microbes did likewise for ammonium. We showed also that niche segregation occurs between phorid and culicid larvae, with the former fragmenting prey carcasses and the latter suppressing fluid microbe levels. These results clarify the relationships between several key pitcher-dwelling organisms, and show that pitcher communities facilitate nutrient sequestration in their host.

Nepenthes gracilis
inquiline
digestive mutualism
phytotelm
plant–microbe interaction


Structural characterization of phenolic compounds and antioxidant activity of the phenolic-rich fraction from defatted adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) seed meal.

Abstract: The current study aims to investigate the antioxidant activities of various extracts from defatted adlay seed meal (DASM) based on the oxygen radical absorbance capacity (ORAC) assay, peroxyl radical scavenging capacity (PSC) assay and cellular antioxidant activity (CAA) assay. Of all the fractions, the n-butanol fraction exhibited the highest antioxidant activity, followed by crude acetone extract and aqueous fractions. Of the three sub-fractions obtained by Sephadex LH-20 chromatography, sub-fraction 3 possessed the highest antioxidant activity and total phenolic content. There was a strong positive correlation between the total phenolic content and the antioxidant activity. Based on HPLC-DAD-ESI-MS/MS analysis, the most abundant phenolic acid in sub-fraction 3 of DASM was ferulic acid at 67.28 mg/g, whereas the predominant flavonoid was rutin at 41.11 mg/g. Of the major individual compounds in sub-fraction 3, p-coumaric acid exhibited the highest ORAC values, and quercetin exhibited the highest PSC values and CAA values.

Wang|Lifeng|L| Chen|Chao|C| Su|Anxiang|A| Zhang|Yiyi|Y| Yuan|Jian|J| Ju|Xingrong|X|

Phenolic compounds
Antioxidant activity
Fractionation
Defatted adlay seed meal
Ferulic acid
Rutin
Quercetin


Adjunctive and novel therapies for sepsis.

Abstract: Primary goals of sepsis therapy include early, appropriate antimicrobial therapy and prompt recognition and reversal of shock. Despite these measures, however, sepsis remains an important source of pediatric morbidity and mortality. Here we review rationale and existing evidence in support of adjunctive sepsis therapies including extracorporeal support, immunomodulation, and mitochondria-targeted therapies. While each of these therapeutic modalities shows promise, additional studies are urgently needed to identify the right patients, the right timing, and the right context for these interventions.

Muszynski|Jennifer A|JA|
Frazier|W Joshua|WJ|
Hall|Mark W|MW|

Sepsis
immunomodulation
extra corporeal membrane oxygenation
mitochondria
renal dialysis


Design and implementation of a low-cost, tabletop MRI scanner for education and research prototyping.

Abstract: While access to a laboratory MRI system is ideal for teaching MR physics as well as many aspects of signal processing, providing multiple MRI scanners can be prohibitively expensive for educational settings. To address this need, we developed a small, low-cost, open-interface tabletop MRI scanner for academic use. We constructed and tested 20 of these scanners for parallel use by teams of 2-3 students in a teaching laboratory. With simplification and down-scaling to a 1 cm FOV, fully-functional scanners were achieved within a budget of $10,000 USD each. The design was successful for teaching MR principles and basic signal processing skills and serves as an accessible testbed for more advanced MR research projects. Customizable GUIs, pulse sequences, and reconstruction code accessible to the students facilitated tailoring the scanner to the needs of laboratory exercise. The scanners have been used by >800 students in 6 different courses and all designs, schematics, sequences, GUIs, and reconstruction code is open-source.

Cooley|Clarissa Zimmerman|CZ|
Stockmann|Jason P|JP|
Witzel|Thomas|T|
LaPierre|Cris|C|
Mareyam|Azma|A|
Jia|Feng|F|
Zaitsev|Maxim|M|
Wenhui|Yang|Y|
Zheng|Wang|W|
Stang|Pascal|P|
Scott|Greig|G|
Adalsteinsson|Elfar|E|
White|Jacob K|JK|
Wald|Lawrence L|LL|


Two Novel Mutations Associated With Ataxia-Telangiectasia Identified Using an Ion AmpliSeq Inherited Disease Panel.

Abstract: Ataxia-telangiectasia (A-T), or Louis-Bar syndrome, is a rare neurodegenerative disorder associated with immunodeficiency. For families with at least one affected child, timely A-T genotyping during any subsequent pregnancy allows the parents to make an informed decision about whether to continue to term when the fetus is affected. Mutations in the ATM gene, which is 150 kb long, give rise to A-T; more than 600 pathogenic variants in ATM have been characterized since 1990 and new mutations continue to be discovered annually. Therefore, limiting genetic screening to previously known SNPs by PCR or hybridization with microarrays may not identify the specific pathogenic genotype in ATM for a given A-T family. However, recent developments in next-generation sequencing technology offer prompt high-throughput full-length sequencing of genomic fragments of interest. This allows the identification of the whole spectrum of mutations in a gene, including any novel ones. We report two A-T families with affected children and current pregnancies. Both families are consanguineous and originate from Caucasian regions of Russia and Azerbaijan. Before our study, no ATM mutations had been identified in the older children of these families. We used ion semiconductor sequencing and an Ion AmpliSeq™ Inherited Disease Panel to perform complete ATM gene sequencing in a single member of each family. Then we compared the experimentally determined genotype with the affected/normal phenotype distribution in the whole family to provide unambiguous evidence of pathogenic mutations responsible for A-T. A single novel SNP was allocated to each family. In the first case, we found a mononucleotide deletion, and in the second, a mononucleotide insertion. Both mutations lead to truncation of the ATM protein product. Identification of the pathogenic mutation in each family was performed in a timely fashion, allowing the fetuses to be tested and diagnosed. The parents chose to continue with both pregnancies as both fetuses had a healthy genotype and thus were not at risk of A-T.

Kuznetsova|Maria V|MV|
Trofimov|Dmitry Yu|DY|
Shubina|Ekaterina S|ES|
Kochetkova|Taisiya O|TO|
Karetnikova|Natalia A|NA|
Barkov|Ilya Yu|IY|
Bakharev|Vladimir A|VA|
Gusev|Oleg A|OA|
Sukhikh|Gennady T|GT|

ataxia-telangiectasia
Louis-Bar syndrome
ATM gene
IDP
prenatal diagnostic


Worked to death? A census-based longitudinal study of the relationship between the numbers of hours spent working and mortality risk.

Abstract:
BACKGROUND
There has been an increasing interest in the health effects of long working hours, but little empirical evidence to substantiate early case series suggesting an increased mortality risk. The aim of the current study is to quantify the mortality risk associated with long working hours and to see if this varies by employment relations and conditions of occupation.

METHODS
A census-based longitudinal study of 414 949 people aged 20-59/64 years, working at least 35 h/week, subdivided into four occupational classes (managerial/professional, intermediate, own account workers, workers in routine occupations) with linkage to deaths records over the following 8.7 years. Cox proportional hazards models were used to examine all-cause and cause-specific mortality risk.

RESULTS
Overall 9.4% of the cohort worked 55 or more h/week, but this proportion was greater in the senior management and professional occupations and in those who were self-employed. Analysis of 4447 male and 1143 female deaths showed that hours worked were associated with an increased risk of all-cause mortality only for men working for more than 55 or more h/week in routine/semi-routine occupations [adjusted hazard ratios (adjHR) 1.31: 95% confidence interval (CI) 1.11, 1.55] compared with their peers working 35-40 h/week. Their equivalent risk of death from cardiovascular disease was (adjHR 1.49: 95% CI 1.10, 2.00).

CONCLUSIONS
These findings substantiate and add to the earlier studies indicating the deleterious impact of long working hours but also suggest that the effects are moderated by employment relations or conditions of occupation. The policy implications of these findings are discussed.

longitudinal study
mortality
Hours worked


ACL injury prevention: Where have we come from and where are we going?

Abstract: Anterior cruciate ligament (ACL) injuries are one of the most common and severe knee injuries across sports. As such, ACL injury prevention has been a focus of research and sports medicine practice for the past three-plus decades. Examining the current research and identifying both clinical strategies and research gaps, the aim of this review is to empower clinicians and researchers with knowledge of where the ACL injury prevention literature is currently and where it is going in the future. This paper examines the mechanism of ACL injury prevention, screening, implementation, compliance, adherence, coronavirus, and areas of future research. Clinical significance: The time lag between research and practical implementation in general healthcare settings can be as long as 17 years; however, athletes playing sports today are unable to wait that long. With effective programs already established, implementation and adherence to these programs is essential. Strategies such as coaching education, increasing awareness of free programs, identifying barriers, and overcoming implementation obstacles through creative collaboration are just a few ways that could help improve both ACL injury prevention implementation and adherence.


Modeling methods for estimating HIV incidence: a mathematical review.

Abstract: Estimating HIV incidence is crucial for monitoring the epidemiology of this infection, planning screening and intervention campaigns, and evaluating the effectiveness of control measures. However, owing to the long and variable period from HIV infection to the development of AIDS and the introduction of highly active antiretroviral therapy, accurate incidence estimation remains a major challenge. Numerous estimation methods have been proposed in epidemiological modeling studies, and here we review commonly-used methods for estimation of HIV incidence. We review the essential data required for estimation along with the advantages and disadvantages, mathematical structures and likelihood derivations of these methods. The methods include the classical back-calculation method, the method based on CD4+ T-cell depletion, the use of HIV case reporting data, the use of cohort study data, the use of serial or cross-sectional prevalence data, and biomarker approach. By outlining the mechanistic features of each method, we provide guidance for planning incidence estimation efforts, which may depend on national or regional factors as well as the availability of epidemiological or laboratory datasets.

D023241:Antiretroviral Therapy, Highly Active

CD4
Biomarker
HIV/AIDS
Mathematical model
statistical estimation


Activation of Microglyocytes in the Anterior Horns of Rat Spinal Cord after Administration of Bacterial Lipopolysaccharide.

Abstract: We studied the reaction of the microglia of the anterior horns of the rat spinal cord to intraperitoneal administration of bacterial LPS. Immunohistochemical analysis showed that acute systemic inflammation leads to activation of more than half of microglial cells as soon as in 24 h after LPS injection, while the total number of microglial cells does not change significantly. It was hypothesized that activated microglial cells are involved in the reorganization of synaptic connections, but do not have a neurotoxic effect.

spinal cord
lipopolysaccharide
microglia
systemic inflammation


Locomotion and cadence detection using a single trunk-fixed accelerometer: validity for children with cerebral palsy in daily life-like conditions.

Abstract:
BACKGROUND
Physical therapy interventions for ambulatory youth with cerebral palsy (CP) often focus on activity-based strategies to promote functional mobility and participation in physical activity. The use of activity monitors validated for this population could help to design effective personalized interventions by providing reliable outcome measures. The objective of this study was to devise a single-sensor based algorithm for locomotion and cadence detection, robust to atypical gait patterns of children with CP in the real-life like monitoring conditions.

METHODS
Study included 15 children with CP, classified according to Gross Motor Function Classification System (GMFCS) between levels I and III, and 11 age-matched typically developing (TD). Six IMU devices were fixed on participant's trunk (chest and low back/L5), thighs, and shanks. IMUs on trunk were independently used for development of algorithm, whereas the ensemble of devices on lower limbs were used as reference system. Data was collected according to a semi-structured protocol, and included typical daily-life activities performed indoor and outdoor. The algorithm was based on detection of peaks associated to heel-strike events, identified from the norm of trunk acceleration signals, and included several processing stages such as peak enhancement and selection of the steps-related peaks using heuristic decision rules. Cadence was estimated using time- and frequency-domain approaches. Performance metrics were sensitivity, specificity, precision, error, intra-class correlation coefficient, and Bland-Altman analysis.

RESULTS
According to GMFCS, CP children were classified as GMFCS I (n = 7), GMFCS II (n = 3) and GMFCS III (n = 5). Mean values of sensitivity, specificity and precision for locomotion detection ranged between 0.93-0.98, 0.92-0.97 and 0.86-0.98 for TD, CP-GMFCS I and CP-GMFCS II-III groups, respectively. Mean values of absolute error for cadence estimation (steps/min) were similar for both methods, and ranged between 0.51-0.88, 1.18-1.33 and 1.94-2.3 for TD, CP-GMFCS I and CP-GMFCS II-III groups, respectively. The standard deviation was higher in CP-GMFCS II-III group, the lower performances being explained by the high variability of atypical gait patterns.

CONCLUSIONS
The algorithm demonstrated good performance when applied to a wide range of gait patterns, from normal to the pathological gait of highly affected children with CP using walking aids.

Paraschiv-Ionescu|Anisoara|A|
Newman|Christopher J|CJ|
Carcreff|Lena|L|
Gerber|Corinna N|CN|
Armand|Stephane|S|
Aminian|Kamiar|K|

Accelerometer
Validation
Cerebral palsy
Atypical gait
Step detection


A Method for Chlorophyll-a and Suspended Solids Prediction through Remote Sensing and Machine Learning.

Abstract: Total Suspended Solids (TSS) and chlorophyll-a concentration are two critical parameters to monitor water quality. Since directly collecting samples for laboratory analysis can be expensive, this paper presents a methodology to estimate this information through remote sensing and Machine Learning (ML) techniques. TSS and chlorophyll-a are optically active components, therefore enabling measurement by remote sensing. Two study cases in distinct water bodies are performed, and those cases use different spatial resolution data from Sentinel-2 spectral images and unmanned aerial vehicles together with laboratory analysis data. In consonance with the methodology, supervised ML algorithms are trained to predict the concentration of TSS and chlorophyll-a. The predictions are evaluated separately in both study areas, where both TSS and chlorophyll-a models achieved R-squared values above 0.8.

chlorophyll-a
remote sensing
machine learning
total suspended solids
water quality
K nearest neighbors
artificial neural networks
random forest


Implicating endothelial cell senescence to dysfunction in the ageing and diseased brain.

Abstract: Cerebrovascular endothelial cells (CECs) are integral components of both the blood-brain barrier (BBB) and the neurovascular unit (NVU). As the primary cell type of the BBB, CECs are responsible for the tight regulation of molecular transport between the brain parenchyma and the periphery. Additionally, CECs are essential in neurovascular coupling where they help regulate cerebral blood flow in response to regional increases in cellular demand in the NVU. CEC dysfunction occurs during both normative ageing and in cerebrovascular disease, which leads to increased BBB permeability and neurovascular uncoupling. This MiniReview compiles what is known about the molecular changes underlying CEC dysfunction, many of which are reminiscent of cells that have become senescent. In general, cellular senescence is defined as an irreversible growth arrest characterized by the acquisition of a pro-inflammatory secretory phenotype in response to DNA damage or other cellular stresses. We discuss evidence for endothelial cell senescence in ageing and cardiovascular disease, and how CEC senescence may contribute to age-related cerebrovascular dysfunction.

D000375:Aging
D001812:Blood-Brain Barrier
D001921:Brain
D002199:Capillary Permeability
D016922:Cellular Senescence
D002561:Cerebrovascular Disorders
D016207:Cytokines
D042783:Endothelial Cells
D006801:Humans
D000069336:Neurovascular Coupling
D009569:Nitric Oxide
D052250:Nitric Oxide Synthase Type III

endothelial cells
cellular senescence
Alzheimer's disease
neurodegenerative disorders
neuropharmacology


A Plasma Cell Myeloma With Post-Therapy Anaplastic Morphology, Osteomyelosclerosis, and Strong Pan-Cytokeratin (AE1/AE3) Expression: A Potential Diagnostic Pitfall.

Abstract: Reactive and neoplastic plasma cells can display considerable morphological anaplasia as well as variable immunoreactivity for epithelial markers including epithelial membrane antigen, pan-cytokeratin (panCK) and high-molecular-weight cytokeratin, potentially creating diagnostic dilemmas. We describe the case of a 51-year-old male, previously treated for IgGλ plasma cell myeloma, whose bone marrow biopsy showed focal replacement by sheets of pleomorphic malignant cells and grade 3 myelofibrosis, raising the morphological possibility of a carcinomatous infiltration. First-line immunohistochemistry revealed strong panCK as well as CD138 positivity. However, subsequent MUM-1 and CD38 stains were also positive, clinching the diagnosis of relapsed plasma cell myeloma with anaplastic morphology and aberrant strong cytokeratin expression. The case warns of the perils of using limited immunohistochemical panels in poorly differentiated metastatic neoplasms and the importance of providing a complete clinical background to the reporting pathologist.

D000971:Antineoplastic Combined Chemotherapy Protocols

plasma cell myeloma
osteomyelosclerosis
pan-cytokeratin
bone marrow infiltration
histomorphology
metastatic tumor


Range extension of Brathinus satoi in China (Coleoptera, Staphylinidae).

Abstract: Brathinus satoi Kishimoto & Shimada, 2003 is recorded from Longwangshan Nature Reserve, Zhejiang, China. Some diagnostic characters of the species are discussed based on more specimens, and some biological notes are made on the species.

Brathinus satoi
China
Coleoptera
Staphylinidae


Modulation of Multivalent Protein Binding on Surfaces by Glycopolymer Brush Chemistry.

Abstract: The presentation of carbohydrates on an array can provide a means to model (mimic) oligosaccharides found on cell surfaces. Tuning the structural features of such carbohydrate arrays can therefore be used to help to elucidate the molecular mechanisms of protein-carbohydrate recognition on cell surfaces. Here we present a strategy to directly correlate the molecular and structural features of ligands presented on a surface with the kinetics and affinity of carbohydrate-lectin binding. The Surface Plasmon Resonance (SPR) spectroscopy analysis identified that by varying the spatial distribution (3D organization) of carbohydrate ligands within the surface grafted polymer layer, the mode of binding changed from multivalent to monovalent: a near 1000-fold change in the equilibrium association constant was achieved. The rupture forces measured by atomic force microscopy (AFM) force spectroscopy also indicated that the mode of binding between lectin and carbohydrate ligands can be modulated by the organization of carbohydrate ligands within the glycopolymer brushes.

Yu|Kai|K|
Creagh|A Louise|AL|
Haynes|Charles A|CA|
Kizhakkedathu|Jayachandran N|JN|

Glycopolymers
Carbohydrate
Lectin
Binding kinetics
Polymer brush


Mental health self-assessment in patients with moderate to severe psoriasis: an observational, multicenter study of 1164 patients in Spain (the VACAP Study).

Abstract:
BACKGROUND
Poor self-assessed mental health appears to be related to the severity of psoriasis.

OBJECTIVE
To evaluate the impact of psoriasis severity on mood and anxiety disorders.

METHODS
A prospective, observational, multicenter study was conducted by 123 dermatologists in Spain. Patients (n=164; mean [SD] age, 45.11 [13.92] years; 60.8% males) with moderate to severe psoriasis were evaluated at baseline and 4 months later. Psoriasis severity was measured using the Psoriasis Area and Severity Index (PASI), with a score range of 0 (mild) to 72 (severe); body surface area involvement (BSA); and physician global assessment (PGA) scores, with a range of 1 (mild) to 7 (severe). Mental health was assessed using the Hospital Anxiety and Depression Scale (HADS), with a total possible score of 0-42 (higher scores representing worse mental health). Mean first and second visit scores were compared.

RESULTS
Mean (SD) scores improved between the first and second visit as follows: 13.24 (9.50) to 5.07 (6.03) for PASI, 12.52 (7.92) to 10.78 (7.32) for overall HADS, 7.83 (4.55) to 6.85 (4.21) for the HADS anxiety subscale, and 4.72 (4.12) to 3.95 (3.76) for the HADS depression subscale (P<.001 in all cases). Multivariate analyses showed that the main factors related to anxiety were psoriasis severity, sex, and completion of graduate studies. The independent variables included in the model for depression were psoriasis severity, sex, and psoriasis located on the head.

CONCLUSIONS
Reductions in disease severity improve self-assessed mood and anxiety disorders in patients with moderate to severe psoriasis.

Pujol|R M|RM|
Puig|L|L|
Daudén|E|E|
Sánchez-Carazo|J L|JL|
Toribio|J|J|
Vanaclocha|F|F|
Yébenes|M|M|
Sabater|E|E|
Casado|M A|MA|
Caloto|M T|MT|
Aragón|B|B|

Escala hospitalaria de ansiedad y depresión


Brazilian red propolis improves cutaneous wound healing suppressing inflammation-associated transcription factor NFκB.

Abstract: The use of natural products in wound healing has been extensively studied in the context of complementary and alternative medicine. Propolis, a natural product, is a polyphenol-rich resin used for this purpose. This study aimed to investigate the effect of Brazilian Red Propolis Extract (BRPE) on inflammation and wound healing in mice, using a tissue repair model. The BRPE polyphenol content was analyzed by liquid chromatography coupled to mass spectrometry (LC/MS). A full-thickness excision lesion was created, and mice were treated orally with daily doses of vehicle solution (water-alcohol solution containing 2% of ethanol, control group) or 100mg/kg of BRPE (P100 group) during nine consecutive days. BRPE chemical composition analysis showed that this complex matrix contains several phenolic compounds such as phenolic acids, phenolic terpenes and flavonoids (especially catechins, flavonols, chalcones, isoflavones, isoflavans, pterocarpans and bioflavonoids). After BRPE administration, it was observed that, when compared to the control group, P100 group presented faster wound closure (p<0.001); less neutrophils per mm2 (p<0.05) and macrophages (p<0.01) in tissue analyses, down regulation of the inflammatory transcription factor pNF-κB protein expression, and reduced production of inflammatory cytokine, such as TGF-β, TNF-α (p<0.0001), and IL-6 (p<0.001). These findings suggest a positive role of BRPE oral administration in the wound healing process via suppressing the inflammatory response during tissue repair.

Corrêa|Flavia Regina Sobreira|FR|
Schanuel|Fernanda Seabra|FS|
Moura-Nunes|Nathalia|N|
Monte-Alto-Costa|Andréa|A|
Daleprane|Julio Beltrame|JB|

Brazilian red propolis
Tissue repair
Polyphenols
InfIammation


Multichromosomal structure and foreign tracts in the Ombrophytum subterraneum (Balanophoraceae) mitochondrial genome.

Abstract: Horizontal gene transfer (HGT) is frequent in parasitic plant mitochondria as a result of vascular connections established in host-parasite relationships. Recent studies of the holoparasitic plant Lophophytum mirabile (Balanophoraceae) revealed the unprecedented acquisition of a large amount of mitochondrial sequences from its legume host. We focused on a close relative, the generalist holoparasite Ombrophytum subterraneum, to examine the incidence of HGT events in the mitochondrial genome (mtDNA). The mtDNA of O. subterraneum assembles into 54 circular chromosomes, only 34 of which contain the 51 full-length coding regions. Numerous foreign tracts (totaling almost 100 kb, ~ 14% of the mtDNA), including 12 intact genes, were acquired by HGT from the Asteraceae hosts. Nine chromosomes concentrate most of those regions and eight are almost entirely foreign. Native homologs of each foreign gene coexist in the mtDNA and are potentially functional. A large proportion of shorter regions were related to the Fabaceae (a total of ~ 110 kb, 15.4%), some of which were shared with L. mirabile. We also found evidence of foreign sequences donated by angiosperm lineages not reported as hosts (Apocynaceae, Euphorbiaceae, Lamiaceae, and Malvales). We propose an evolutionary hypothesis that involves ancient transfers from legume hosts in the common ancestor of Ombrophytum and Lophophytum followed by more recent transfer events in L. mirabile. Besides, the O. subterraneum mtDNA was also subjected to additional HGT events from diverse angiosperm lineages, including large and recent transfers from the Asteraceae, and also from Lamiaceae.

D031111:Balanophoraceae
D004272:DNA, Mitochondrial
D022761:Gene Transfer, Horizontal
D054629:Genome, Mitochondrial

Horizontal gene transfer
Holoparasite
Lophophytum
mtDNA
Evolution
Santalales


Steroidal Saponins from the Rhizomes of Anemarrhena asphodeloides.

Abstract: Four new steroid saponins 1-4 were isolated from the rhizomes of Anemarrhena asphodeloides (Asparagaceae), as well as four known saponins: anemarsaponin B (5) timosaponin D (6), timosaponin E1 (7) anemarsaponin B II (8). Their structures were established through UV and NMR as well as MS data. All the compounds were evaluated for cytotoxicity against HepG2 and SGC7901 human cancer lines. Compounds 3 and 7 displayed medium antiproliferative activities on HepG2 and SGC7901 cells, with IC50 values of 43.90 and 57.90 μM, respectively.

Yang|Bing-You|BY| Zhang|Jing|J| Liu|Yan|Y| Kuang|Hai-Xue|HX|

Asparagaceae
cytotoxicity
MTT
furostanol saponin
spirostanol saponin


Female Sex Pheromone in Trails of the Minute Pirate Bug, Orius minutus (L).

Abstract: Orius minutus (L.) (Heteroptera: Anthocoridae) is a natural enemy of agricultural pests such as thrips, aphids, and various newly hatched insect juveniles. In this study, we conducted 1) behavioral assays for evidence of contact sex pheromone activity in trails of O. minutus, and 2) chemical analysis to identify the essential chemical components of the trails. Males showed arrestment to trails of mature virgin females but not to trails from either conspecific nymphs or immature females. Females also showed arrestment to trails from conspecific males, although the response was weaker than that exhibited by males. The activity of female trails lasted for at least 46 h after deposition. Males showed a response irrespective of mating experience. Following confirmation that a contact sex pheromone was present in the trails of female O. minutus, we used a bioassay-driven approach to isolate the active chemicals. After fractionation on silica gel, the n-hexane fraction was found to be biologically active to males. A major compound in the active fraction was (Z)-9-nonacosene; this compound was found only in trail extracts of mature virgin females. Synthetic (Z)-9-nonacosene arrested O. minutus males, indicating that it is the major active component of the contact sex pheromone in the trails of female O. minutus.

Maeda|Taro|T| Fujiwara-Tsujii|Nao|N| Yasui|Hiroe|H| Matsuyama|Shigeru|S|

Sex pheromone
Trail
Heteroptera
Anthocoridae
Natural enemy
Mating behavior
Monoalkene


Cutaneous manifestation of leukaemia on the penis-the possible implications.

Abstract: Skin lesions are common in the patients with chronic lymphoid leukaemia (CLL); however, leukaemia cutis is a cutaneous manifestation secondary to any form of leukaemia and generally an uncommon phenomenon. They typically present on the face and neck as well as exposed areas. Our case looks at a 78-year-old gentleman with known CLL who presented with an asymptomatic raised lesion in his inner prepuce. The lesion was excised and his immunohistology staining confirms expression of CD5, CD20 and CD23 that is consistent with cutaneous manifestation of CLL. This case highlights the importance of taking leukaemia cutis into consideration in patients with known CLL with unusual features.


Role of Neutrophils in Cardiac Injury and Repair Following Myocardial Infarction.

Abstract: Neutrophils are first-line responders of the innate immune system. Following myocardial infarction (MI), neutrophils are quickly recruited to the ischemic region, where they initiate the inflammatory response, aiming at cleaning up dead cell debris. However, excessive accumulation and/or delayed removal of neutrophils are deleterious. Neutrophils can promote myocardial injury by releasing reactive oxygen species, granular components, and pro-inflammatory mediators. More recent studies have revealed that neutrophils are able to form extracellular traps (NETs) and produce extracellular vesicles (EVs) to aggravate inflammation and cardiac injury. On the contrary, there is growing evidence showing that neutrophils also exert anti-inflammatory, pro-angiogenic, and pro-reparative effects, thus facilitating inflammation resolution and cardiac repair. In this review, we summarize the current knowledge on neutrophils' detrimental roles, highlighting the role of recently recognized NETs and EVs, followed by a discussion of their beneficial effects and molecular mechanisms in post-MI cardiac remodeling. In addition, emerging concepts about neutrophil diversity and their modulation of adaptive immunity are discussed.

neutrophil
myocardial infarction
inflammation
cardiac remodeling
adaptive immunity
angiogenesis
macrophage


Combined Raman- and AFM-based detection of biochemical and nanomechanical features of endothelial dysfunction in aorta isolated from ApoE/LDLR-/- mice.

Abstract: Endothelial dysfunction is recognized as a critical condition in the development of cardiovascular disorders. This multifactorial process involves changes in the biochemical and mechanical properties of endothelial cells leading to disturbed release of vasoprotective mediators. Hypercholesterolemia and increased stiffness of the endothelial cortex are independently shown to result in reduced release of nitric oxide and thus endothelial dysfunction. However, direct evidence linking these parameters to each other is missing. Here, a novel method combining Raman spectroscopy for biochemical analysis and Atomic Force Microscopy (AFM) for analyzing the endothelial nanomechanics was established. Using this dual approach, the same areas of native ex vivo aortas were investigated, either derived from mice with endothelial dysfunction (ApoE/LDLR-/-) or wild type mice. In particular an increased intracellular lipid content and elevated cortical stiffness/elasticity were shown in ApoE/LDLR-/- aortas, demonstrating a direct link between endothelial dysfunction, the biochemical composition and the nanomechanical properties of endothelial cells.

Maase|Martina|M|
Rygula|Anna|A|
Pacia|Marta Z|MZ|
Proniewski|Bartosz|B|
Mateuszuk|Lukasz|L|
Sternak|Magdalena|M|
Kaczor|Agnieszka|A|
Chlopicki|Stefan|S|
Kusche-Vihrog|Kristina|K|

D000818:Animals
D001011:Aorta
D001057:Apolipoproteins E
D004730:Endothelium, Vascular
D051379:Mice
D008810:Mice, Inbred C57BL
D018345:Mice, Knockout
D018625:Microscopy, Atomic Force
D011973:Receptors, LDL
D013059:Spectrum Analysis, Raman

Endothelial dysfunction
Hypercholesterolemia
Raman spectroscopy
Atomic force microscopy
Endothelial nanomechanics


Cereal Intake Increases and Dairy Products Decrease Risk of Cognitive Decline among Elderly Female Japanese.

Abstract:
BACKGROUND
If cognitive decline can be prevented through changes in daily diet with no medical intervention, it will be highly significant for dementia prevention.

OBJECTIVES
This longitudinal study examined the associations of different food intakes on cognitive decline among Japanese subjects.

DESIGN
Prospective cohort study.

SETTING
The National Institute for Longevity Sciences - Longitudinal Study of Aging, a community-based study.

PARTICIPANTS
Participants included 298 males and 272 females aged 60 to 81 years at baseline who participated in the follow-up study (third to seventh wave) at least one time.

MEASUREMENTS
Cognitive function was assessed with the Mini-Mental State Examination (MMSE) in all study waves. Nutritional intake was assessed using a 3-day dietary record in the second wave. Cumulative data among participants with an MMSE >27 in the second wave were analyzed using a generalized estimating equation. Multivariate adjusted odds ratios (OR) and 95% confidence intervals (CI) for an MMSE score ≤27 in each study wave according to a 1 standard deviation (SD) increase of each food intake at baseline were estimated, after adjusting for age, follow-up time, MMSE score at baseline, education, body mass index, annual household income, current smoking status, energy intake, and history of diseases.

RESULTS
In men, after adjusting for age, and follow-up period, MMSE score at baseline, the adjusted OR for a decline in MMSE score was 1.20 (95% CI, 1.02-1.42; p=0.032) with a 1-SD increase in cereal intake. After adjusting for education and other confounding variables, the OR for a decrease in MMSE score did not reach statistical significance for this variable. In women, multivariate adjusted OR for MMSE decline was 1.43 (95% CI, 1.15-1.77; p=0.001) with a 1-SD increase in cereal intake and 0.80 (95% CI, 0.65-0.98; p=0.034) with a 1-SD increase in milk and dairy product intake.

CONCLUSIONS
This study indicates that a 1-SD (108 g/day) decrease in cereal intake and a 1-SD (128 g/day) increase in milk and dairy product intake may have an influence of cognitive decline in community-dwelling Japanese women aged 60 years and older. Further studies are needed in order to explore the potential causal relationship.

Otsuka|R|R| Kato|Y|Y| Nishita|Y|Y| Tange|C|C| Nakamoto|M|M| Tomida|M|M| Imai|T|T| Ando|F|F| Shimokata|H|H|

Cereal
elderly
Japanese
diet
milk and dairy products


Malnutrition or frailty? Overlap and evidence gaps in the diagnosis and treatment of frailty and malnutrition.

Abstract: There is increasing awareness of the detrimental health impact of frailty on older adults and of the high prevalence of malnutrition in this segment of the population. Experts in these 2 arenas need to be cognizant of the overlap in constructs, diagnosis, and treatment of frailty and malnutrition. There is a lack of consensus regarding the definition of malnutrition and how it should be assessed. While there is consensus on the definition of frailty, there is no agreement on how it should be measured. Separate assessment tools exist for both malnutrition and frailty; however, there is intersection between concepts and measures. This narrative review highlights some of the intersections within these screening/assessment tools, including weight loss/decreased body mass, functional capacity, and weakness (handgrip strength). The potential for identification of a minimal set of objective measures to identify, or at least consider risk for both conditions, is proposed. Frailty and malnutrition have also been shown to result in similar negative health outcomes and consequently common treatment strategies have been studied, including oral nutritional supplements. While many of the outcomes of treatment relate to both concepts of frailty and malnutrition, research questions are typically focused on the frailty concept, leading to possible gaps or missed opportunities in understanding the effect of complementary interventions on malnutrition. A better understanding of how these conditions overlap may improve treatment strategies for frail, malnourished, older adults.

Laur|Celia V|CV|
McNicholl|Tara|T|
Valaitis|Renata|R|
Keller|Heather H|HH|

malnutrition
frailty
diagnosis
older adult
assessment
screening
handgrip strength
oral nutritional supplements
diagnostic
dépistage
force de préhension manuelle
fragilité
personne âgée
suppléments nutritionnels oraux
évaluation


Optimizing Anticoagulation Management Through the Use of a Hospital Engagement Network Metric for Inpatient Anticoagulant-Associated Hemorrhage.

Abstract:
BACKGROUND
The University HealthSystem Consortium (UHC), a national hospital engagement network (HEN), establishes health-system metrics to assess and improve quality of care. In 2012, a metric for inpatient anticoagulant hemorrhage was developed. The utility of this metric to improve anticoagulation care has not been assessed.

OBJECTIVE
To identify opportunities to improve anticoagulation safety through the use of a HEN metric for inpatient anticoagulant-associated hemorrhage.

METHODS
This was a single-center, retrospective, observational study of metric identified patients with presumed inpatient anticoagulant hemorrhage. Records were reviewed to confirm anticoagulant hemorrhage and identify bleed site and severity. A structured process was used to assess bleed preventability and subsequently identify opportunities for improving care. Each bleed was reviewed by 2 investigators.

RESULTS
Anticoagulant hemorrhage was confirmed in 85.9% (61/71) with heparin infusion the most common anticoagulant. Patients were primarily medical, with a mean age of 72.7 ± 15 years. The most common bleed sites were gastrointestinal (24.6%) and retroperitoneal (21.3%). Major bleeding occurred in 60.7% (37/61). Anticoagulant hemorrhage was preventable in 18% (11/61) of cases with heparin protocol noncompliance the most common cause of a preventable bleed. Several opportunities for improving heparin infusion therapy were recognized and protocol changes were implemented.

CONCLUSIONS
The UHC metric accurately captures inpatient anticoagulant-associated hemorrhage the majority of time. The UHC metric on anticoagulant-associated hemorrhage can be a useful part of a health system's overall plan for the safe use of anticoagulants in the hospital setting.

Hoffman|Janet L|JL|
Aneese|Nadia J|NJ|
Schmidt|Kyle J|KJ|
Chaben|Alex C|AC|
Smythe|Maureen A|MA|

anticoagulants
anticoagulants
adverse drug reactions
anticoagulation
medication safety
quality assurance


Local Morphological Response of the Distal Femoral Articular-Epiphyseal Cartilage Complex of Young Foals to Surgical Stab Incision and Potential Relevance to Cartilage Injury and Repair in Children.

Abstract:
OBJECTIVE
Describe the local morphological response of the articular-epiphyseal cartilage complex to surgical stab incision in the distal femur of foals, with emphasis on the relationship between growth cartilage injury, enchondral ossification, and repair.

DESIGN
Nine foals were induced into general anesthesia at the age of 13 to 15 days. Four full-thickness stab incision defects were created in the cartilage on the lateral aspect of the lateral trochlear ridge of the left distal femur. Follow-up examination was carried out from 1 to 49 days postoperatively, including examination of intact bones, sawed slabs, and histological sections.

RESULTS
Incision defects filled with cells displaying fibroblast-, chondrocyte-, and osteoblast-like characteristics, potentially validating the rationale behind the drilling of stable juvenile osteochondritis dissecans lesions in children. Incisions induced necrosis within the cartilage on the margins at all depths of the defects. Sharp dissection may therefore be contraindicated in cartilage repair in young individuals. Incisions caused a focal delay in enchondral ossification in 2 foals, apparently related to the orientation of the incision defect relative to the direction of ossification. Defects became progressively surrounded by subchondral bone, in which granulation tissue containing clasts and foci of osteoblast-like cells was observed. Continued enchondral ossification was therefore likely to result in healing of uncomplicated defects to morphologically normal bone.

CONCLUSIONS
Epiphyseal growth cartilage injury had the potential to exert a negative effect on enchondral ossification. Enchondral ossification exerted a beneficial effect on repair. This relationship warrants consideration in future studies of cartilage injury and repair within the articular-epiphyseal cartilage complex of all species.

Olstad|Kristin|K|
Hendrickson|Eli H S|EH|
Ekman|Stina|S|
Carlson|Cathy S|CS|
Dolvik|Nils I|NI|

enchondral ossification
cartilage repair
articular–epiphyseal cartilage complex
cartilage incision
histological examination


Corrigendum: Distribution Patterns of DNA N6-Methyladenosine Modification in Non-coding RNA Genes.

Abstract: [This corrects the article DOI: 10.3389/fgene.2020.00268.].

Li|Yu|Y| Zhang|Xiao-Ming|XM| Luan|Mei-Wei|MW| Xing|Jian-Feng|JF| Chen|Jianguo|J| Xie|Shang-Qian|SQ|

non-coding RNAs
6mA modification
DNA methylation
gene expression
model species


Oestrogen receptor negativity in breast cancer: a cause or consequence?

Abstract: Endocrine resistance, which occurs either by de novo or acquired route, is posing a major challenge in treating hormone-dependent breast cancers by endocrine therapies. The loss of oestrogen receptor α (ERα) expression is the vital cause of establishing endocrine resistance in this subtype. Understanding the mechanisms that determine the causes of this phenomenon are therefore essential to reduce the disease efficacy. But how we negate oestrogen receptor (ER) negativity and endocrine resistance in breast cancer is questionable. To answer that, two important approaches are considered: (1) understanding the cellular origin of heterogeneity and ER negativity in breast cancers and (2) characterization of molecular regulators of endocrine resistance. Breast tumours are heterogeneous in nature, having distinct molecular, cellular, histological and clinical behaviour. Recent advancements in perception of the heterogeneity of breast cancer revealed that the origin of a particular mammary tumour phenotype depends on the interactions between the cell of origin and driver genetic hits. On the other hand, histone deacetylases (HDACs), DNA methyltransferases (DNMTs), miRNAs and ubiquitin ligases emerged as vital molecular regulators of ER negativity in breast cancers. Restoring response to endocrine therapy through re-expression of ERα by modulating the expression of these molecular regulators is therefore considered as a relevant concept that can be implemented in treating ER-negative breast cancers. In this review, we will thoroughly discuss the underlying mechanisms for the loss of ERα expression and provide the future prospects for implementing the strategies to negate ER negativity in breast cancers.

Gajulapalli|Vijaya Narasihma Reddy|VNR|
Malisetty|Vijaya Lakshmi|VL|
Chitta|Suresh Kumar|SK|
Manavathi|Bramanandam|B|

D015972:Gene Expression Regulation, Neoplastic

D064726:Triple Negative Breast Neoplasms

oestrogen receptor (ER)-negative breast cancer


Isolation and Molecular Typing of Leishmania infantum from Phlebotomus perfiliewi in a Re-Emerging Focus of Leishmaniasis, Northeastern Italy.

Abstract: Visceral leishmaniasis (VL) caused by Leishmania (L.) infantum is a public health threat in the Emilia-Romagna region, northeastern Italy, but its epidemiology has not been fully elucidated in this area. The objective of this study was to characterize Leishmania infection in sand flies collected in a re-emerging focus of VL in the Bologna province. During the summer of 2016, 6114 sand flies were collected, identified, and tested for Leishmania detection. Of the identified sand flies, 96.5% were Phlebotomus (P.) perfiliewi and 3.5% were P. perniciosus. Detected parasites were characterized by biomolecular methods (multilocus microsatellite typing and characterization of repetitive region on chromosome 31), and quantified by real-time PCR. The prevalence of Leishmania infection in individually-tested P. perfiliewi sand flies varied from 6% to 10% with an increasing trend during the season. Promastigotes of L. infantum were isolated by dissection in one P. perfiliewi female; the isolated strain (Lein-pw) were closely related to Leishmania parasites from VL cases in northeastern Italy, but differed from strains isolated in dogs from the same area. Our findings strongly support the vector status of P. perfiliewi for human VL in the study area.

Calzolari|Mattia|M| Carra|Elena|E| Rugna|Gianluca|G| Bonilauri|Paolo|P| Bergamini|Federica|F| Bellini|Romeo|R| Varani|Stefania|S| Dottori|Michele|M|

molecular typing
Leishmania infantum
Phlebotomus perfiliewi
visceral leishmaniasis
Phlebotomus perniciosus
parasite load
quantitative PCR.


Incidence of frailty among community-dwelling older adults: a nationally representative profile in China.

Abstract:
BACKGROUND
Frailty is a clinically recognizable state of reduced resilience to stressors and increased vulnerability to adverse outcomes. The majority of studies have focused on the prevalence and risk factors of frailty, while the incidence of frailty has not been well documented, especially in less developed regions including China-a country that has the largest aging population in the world. We investigated the incidence of frailty among non-frail Chinese older adults by sociodemographic characteristics, disease burden, and geographic region.

METHODS
Participants were 4939 adults aged ≥60 years from the China Health and Retirement Longitudinal Study, a cohort study of a nationally representative sample of middle-aged and older community-dwelling adults from 28 provinces in China. Frailty was assessed by an adapted version of the well-validated Fried's physical frailty phenotype, in which five criteria were included: weakness, slowness, exhaustion, physical inactivity, and shrinking.

RESULTS
Over an average of 2.1 years of follow-up (10,514.2 person-years), the weighted incidence rate of frailty was 60.6 per 1000 person-years; the incidence rate was 28.8 and 86.6 per 1000 person-years for those who were initially robust and prefrail, respectively. Participants who were older and widowed, had lower education and household income, lived in rural areas, and had higher burden of chronic conditions had higher frailty incidence. Frailty incidence ranged from 44.8 per 1000 person-years in the Southeast to 93.0 per 1000 person-years in the Northwest.

CONCLUSIONS
Incidence rate of frailty was 60.6 per 1000 person-years among community-living Chinese adults aged ≥ 60 years. Substantial sociodemographic and geographical disparities exist in frailty incidence.

Frailty
China
Epidemiology
Health disparities


Concordance between independent first-line assessors in the Western Australian Audit of Surgical Mortality.

Abstract:
BACKGROUND
The Western Australian Audit of Surgical Mortality independently reviews deaths under a surgeon. A surgical case form completed by the surgeon is reviewed by a first-line assessor, who in 15% of cases recommends a detailed case note review. The aim of this study was to assess concordance between the original first-line assessment (FLA1) and an additional first-line assessment (FLA2) in relation to the necessity for a second-line assessment (SLA) and recognition of clinical management issues.

METHODS
Experienced assessors (n = 39) representing different specialties were invited to be FLA2 assessors for 170 random cases. They completed identical FLA forms. FLA2 assessors were consultant surgeons with experience of the peer review process. Inter-rater reliability was determined using Gwet's Agreement Coefficient.

RESULTS
A total of 170 FLAs, 158 (92.9%) were returned. Concordance between assessors for recommending an SLA was 70.5% (110 of 156), with an SLA recommended for 16 (10.3%) FLAs but not for 94 (60.3%) FLAs. When there was non-concordance, FLA1 assessors requested more SLAs than did FLA2 assessors (29 [18.6%] vs. 17 [10.9%], respectively). Three adverse events were recorded. In two the assessors differed, with one recording an adverse event and the other recording an area of concern. Additionally, in one instance the assessors differed on the preventability of the event but agreed that it may have contributed to death.

CONCLUSION
Concordance between assessors ranged between 'moderate' (requests for an SLA) through to 'almost perfect' (presence of an adverse event). This suggests the FLA screening process is reliable.


Leaf Lipid Alterations in Response to Heat Stress of Arabidopsis thaliana.

Abstract: In response to elevated temperatures, plants alter the activities of enzymes that affect lipid composition. While it has long been known that plant leaf membrane lipids become less unsaturated in response to heat, other changes, including polygalactosylation of galactolipids, head group acylation of galactolipids, increases in phosphatidic acid and triacylglycerols, and formation of sterol glucosides and acyl sterol glucosides, have been observed more recently. In this work, by measuring lipid levels with mass spectrometry, we confirm the previously observed changes in Arabidopsis thaliana leaf lipids under three heat stress regimens. Additionally, in response to heat, increased oxidation of the fatty acyl chains of leaf galactolipids, sulfoquinovosyldiacylglycerols, and phosphatidylglycerols, and incorporation of oxidized acyl chains into acylated monogalactosyldiacylglycerols are shown. We also observed increased levels of digalactosylmonoacylglycerols and monogalactosylmonoacylglycerols. The hypothesis that a defect in sterol glycosylation would adversely affect regrowth of plants after a severe heat stress regimen was tested, but differences between wild-type and sterol glycosylation-defective plants were not detected.

Shiva|Sunitha|S|
Samarakoon|Thilani|T|
Lowe|Kaleb A|KA|
Roach|Charles|C|
Vu|Hieu Sy|HS|
Colter|Madeline|M|
Porras|Hollie|H|
Hwang|Caroline|C|
Roth|Mary R|MR|
Tamura|Pamela|P|
Li|Maoyin|M|
Schrick|Kathrin|K|
Shah|Jyoti|J|
Wang|Xuemin|X|0000-0002-6251-6745
Wang|Haiyan|H|
Welti|Ruth|R|0000-0003-4373-2538

heat stress
Arabidopsis thaliana
phosphatidic acid
triacylglycerols
sterol glucosides
acyl sterol glucosides
acylated lipids
lipidomics
oxidized lipids
polygalactosylated lipids