Activities of daily living, life orientation, and health-related quality of life among older people in nursing homes: a national cross-sectional study in China.

Abstract:
PURPOSE
This study aimed to explore the current status of activities of daily living (ADLs), life orientation, and health-related quality of life (HRQoL) among older people in nursing homes and to further examine the mediating role of life orientation in the impact of ADLs on HRQoL.

METHODS
A national cross-sectional study was conducted among older people aged 60 and above in nursing homes by the randomly stratified cluster sampling method. The status of ADLs, life orientation and HRQoL were measured using an ADL scale, a life orientation scale and the SF-12v2 scale, respectively. Multiple linear regression models were used to identify explanatory factors associated with ADLs, life orientation, and HRQoL. The potential mediating role of life orientation in the relationship between ADL and HRQoL was explored by mediation analysis.

RESULTS
The overall prevalence of ADL disability was 52.67%, and 84.37% of older people in nursing homes had a negative life orientation. The mean scores of physical health and mental health among older people in nursing homes were 45.44 ± 6.46 and 42.67 ± 8.48, respectively. Some sociodemographic characteristics were associated with poor physical health and mental health. After adjustments were made for covariates, the life orientation score mediated 13.81% of the total effect of the ADL score on physical component score of HRQoL and mediated 45.33% of the mental component score of HRQoL.

CONCLUSION
A sizeable proportion of older people had ADL disability and negative life orientation, and HRQoL was poor among older Chinese people in nursing homes. Life orientation partially mediates the relationship between ADLs and HRQoL.

Activities of daily living
Life orientation
Health-related quality of life
Older people
Nursing homes


Effects of Echinostoma trivolvis metacercariae infection during development and metamorphosis of the wood frog (Lithobates sylvaticus).

Abstract: Many organisms face energetic trade-offs between defense against parasites and other host processes that may determine overall consequences of infection. These trade-offs may be particularly evident during unfavorable environmental conditions or energetically demanding life history stages. Amphibian metamorphosis, an ecologically important developmental period, is associated with drastic morphological and physiological changes and substantial energetic costs. Effects of the trematode parasite Echinostoma trivolvis have been documented during early amphibian development, but effects during later development and metamorphosis are largely unknown. Using a laboratory experiment, we examined the energetic costs of late development and metamorphosis coupled with E. trivolvis infection in wood frogs, Lithobates [=Rana] sylvaticus. Echinostoma infection intensity did not differ between tadpoles examined prior to and after completing metamorphosis, suggesting that metacercariae were retained through metamorphosis. Infection with E. trivolvis contributed to a slower growth rate and longer development period prior to the initiation of metamorphosis. In contrast, E. trivolvis infection did not affect energy expenditure during late development or metamorphosis. Possible explanations for these results include the presence of parasites not interfering with pronephros degradation during metamorphosis or the mesonephros compensating for any parasite damage. Overall, the energetic costs of metamorphosis for wood frogs were comparable to other species with similar life history traits, but differed from a species with a much shorter duration of metamorphic climax. Our findings contribute to understanding the possible role of energetic trade-offs between parasite defense and host processes by considering parasite infection with simultaneous energetic demands during a sensitive period of development.

Orlofske|Sarah A|SA|
Belden|Lisa K|LK|
Hopkins|William A|WA|

Parasite
Trematode
Tadpole
Energy
Metamorphic climax
Metabolism
Oxygen consumption
Respiration


Gait characteristics of post-poliomyelitis patients: standardization of quantitative data reporting.

Abstract:
OBJECTIVES
To evaluate the differences in gait characteristics and gait symmetry of post-polio syndrome (PPS) patients ambulating with or without shoes and between subgroups walking with different walking aids and orthoses, study the correlation of these data with personal data, illness condition, physical health, frequency of using aids and orthotics and frequency of falls, and derive recommendations for standardization of reporting these data.

METHOD
Twenty-six PPS subjects ambulated with their own walking devices. We calculated spatio-temporal parameters and symmetry indices (SI) of gait using a data acquired by a motion capture system. We compared inter-subject differences in gait pattern for PPS groups that differed by questionnaire-obtained data of demographics, physical activity, polio history, falls and walking aids. Additional inter-subject comparisons were performed between normal subjects (n=16), PPS patients walking with shoes with/without an ankle-foot-orthosis (n=11), PPS patients walking with knee-ankle-foot-orthosis (n=5), and PPS patients walking with a walker/crutches (n=10). We also compared intra-subject variability in PPS subjects who were able to repeat the trials barefoot.

RESULTS
Our main results show that subjects who reported participating in physical activity twice a week or more had significantly better step time and double support symmetry. Subjects who use walking aids on a daily basis had significantly higher gait cadence and shorter stride time. Also, subjects that do not require knee-ankle-foot orthoses and/or walking aids walked with a smaller base width and better symmetry in stance and swing durations than PPS subjects who require these aids.

CONCLUSIONS
The gait pattern of PPS patients is related to numerous intrinsic and extrinsic factors. Standardization of the reporting protocol of gait-related data of PPS patients is crucial for patient evaluation and treatment design.

Analyse informatique comparative de la démarche


Higher Rate of Return to Preinjury Activity Levels After Anterior Cruciate Ligament Reconstruction With a Bone-Patellar Tendon-Bone Versus Hamstring Tendon Autograft in High-Activity Patients: Results From the New Zealand ACL Registry.

Abstract:
BACKGROUND
In primary anterior cruciate ligament (ACL) reconstruction, a bone-patellar tendon-bone (BTB) autograft is associated with lower ipsilateral failure rates. BTB autografts are associated with a higher rate of contralateral ACL injuries, which some clinicians view as a marker of success of the BTB autograft. However, there is a lack of evidence on whether BTB autografts improve the rate of return to activity and sport.

PURPOSE
To compare the rate of return to preinjury activity levels in high-activity patients after ACL reconstruction with BTB autograft or hamstring tendon autograft.

STUDY DESIGN
Cohort study; Level of evidence, 3.

METHODS
In a high-activity cohort of patients recorded between 2014 and 2018 in the New Zealand ACL Registry, prospectively collected data on preinjury and postoperative Marx activity scores were analyzed. The proportion of patients who returned to their preinjury activity levels at 1- and 2-year follow-up was compared between graft types.

RESULTS
Overall, 11.3% (208/1844) of patients returned to their preinjury activity levels at 1-year follow-up, and 15.5% (184/1190) returned at 2-year follow-up. At 1-year follow-up, 17.2% of patients with a BTB autograft returned to their preinjury activity levels compared with 9.3% of patients with a hamstring tendon autograft (adjusted odds ratio, 1.59 [95% CI, 1.16-2.17]; P = .004). At 2-year follow-up, 23.3% of patients with a BTB autograft had returned to their preinjury activity levels compared with 13.3% of patients with a hamstring tendon autograft (adjusted odds ratio, 1.63 [95% CI, 1.14-2.34]; P = .008). Male sex and younger age were associated with a higher rate of return to activity at both follow-up time points.

CONCLUSION
The use of BTB autografts increased the odds of returning to preinjury activity levels at early follow-up. A higher rate of return to activity is a possible explanation for the higher rate of contralateral ACL injuries with the use of BTB autografts.

Rahardja|Richard|R|0000-0003-2634-3813
Love|Hamish|H|
Clatworthy|Mark G|MG|
Monk|Andrew Paul|AP|
Young|Simon W|SW|

D000070598:Anterior Cruciate Ligament Injuries

D059549:Anterior Cruciate Ligament Reconstruction

anterior cruciate ligament
return to activity
ACL reconstruction
graft
return to sport
sports medicine


Left Anterior Descending Coronary Artery and Multiple Peripheral Mycotic Aneurysms Due to Mycobacterium Bovis Following Intravesical Bacillus Calmette-Guerin Therapy: A Case Report.

Abstract: The use of live attenuated intravesicular Bacillus Calmette-Guerin (BCG) therapy is a generally accepted safe and effective method for the treatment of superficial transitional cell carcinoma (TCC) of the bladder. Although rare, < 5% of patient's treated with intravesicular BCG therapy may develop potentially serious complications, including localized infections to the genitourinary tract, mycotic aneurysms and osteomyelitis. We present here a case of a 63-year-old male who developed left coronary and multiple peripheral M. Bovis mycotic aneurysms as a late complication of intravesicular BCG therapy for superficial bladder cancer. The patient initially presented with acute onset pain and swelling in the left knee > 2 years following initial therapy, and initial workup revealed a ruptured saccular aneurysm of the left popliteal artery as well as incidental bilateral common femoral artery aneurysms. Following endovascular treatment and additional workup, the patient was discovered to have additional aneurysms in the right popliteal artery and left anterior descending artery (LAD). Surgical pathology and bacterial cultures obtained from the excised femoral aneurysms and surgical groin wounds were positive for Mycobacterium Bovis, and the patient was initiated on a nine-month antimycobacterial course of isoniazid, rifampin and ethambutol. Including the present case, there has been a total of 32 reported cases of mycotic aneurysms as a complication from intravesicular BCG therapy, which we will review here. The majority of reported cases involve the abdominal aorta; however, this represents the first known reported case of a coronary aneurysm.

D000283:Administration, Intravesical
D000785:Aneurysm, Infected
D000995:Antitubercular Agents
D001500:BCG Vaccine
D002295:Carcinoma, Transitional Cell
D003323:Coronary Aneurysm
D057510:Endovascular Procedures
D005263:Femoral Artery
D006801:Humans
D008297:Male
D008875:Middle Aged
D009163:Mycobacterium bovis
D011150:Popliteal Artery
D001749:Urinary Bladder Neoplasms

Mycotic aneurysm
Mycobacterium bovis
Bacillus Calmette-Guerin
intravesicular
BCG


Nation-wide survey of advanced non-melanoma skin cancers treated at dermatology departments in Japan.

Abstract:
BACKGROUND
There are limited treatment options for advanced non-melanoma skin cancers (NMSCs). To overcome this issue, we need to conduct clinical studies, however, there is a lack of information on how many patients with advanced NMSCs are treated annually in Japan.

OBJECTIVE
To investigate the actual number of advanced NMSC patients in Japan.

METHODS
A questionnaire survey was sent to 668 institutes to educe information on: 1) the numbers of patients with squamous cell carcinoma (SCC), extramammary Paget disease (EMPD), other skin origin carcinomas, and cutaneous angiosarcoma (CAS) admitted in 2016 and 2017; 2) the preferred first- and second-line chemotherapies; and 3) the anticipated for future development.

RESULTS
Questionnaires were returned from 383 (57.3%) institutes. They reported a total of 1765 patients over the 2 years. The annual number patients with SCC, EMPD, other skin carcinomas, and CAS was 323.5, 192.5, 126, and 240.5, respectively. We estimated the annual number of patients for all 668 institutes to be 1255.6. Current first- and second-line treatment for NMSCs were chemotherapy regimens, but immune checkpoint inhibitors were the most anticipated new drugs for SCC and CAS, while chemotherapy was still the most anticipated treatment for EMPD.

CONCLUSION
Considering that during 2017, the number of deaths in Japan due to NMSC was reported to be 948, our estimated annual number of patients with NMSCs, 1255.6 seems to be an accurate estimation. As most of the treatment options for advanced NMSCs are outdated, the results of this study should be used to propose clinical studies.

Fujisawa|Yasuhiro|Y| Funakoshi|Takeru|T| Nakamura|Yoshiyuki|Y| Ishii|Maki|M| Asai|Jun|J| Shimauchi|Takatoshi|T| Fujii|Kazuyasu|K| Fujimoto|Manabu|M| Katoh|Norito|N| Ihn|Hironobu|H|

D000971:Antineoplastic Combined Chemotherapy Protocols

D061025:Costimulatory and Inhibitory T-Cell Receptors

Advanced
Non-melanoma skin cancer
Treatment
Squamous cell carcinoma
Extramammary Paget disease
Cutaneous angiosarcoma
Incidence


Body mass index and vitamin D level in carpal tunnel syndrome patients.

Abstract:
Background
Carpal tunnel syndrome (CTS) is the most common entrapment neuropathy of the upper extremity. The aim of this study is to evaluate the body mass index (BMI) and vitamin D levels in CTS patients.

Methods
The current study was conducted at Zagazig University Hospitals. It included 50 CTS patients and 50 controls. Clinical assessment was carried out to exclude symptoms and signs of neuropathy. Laboratory investigations including vitamin D levels, glycosylated hemoglobin, liver, and kidney function were carried out for every participant. All patients underwent electrodiagnostic study and completed Boston questionnaire to assess their pain sum score, symptom severity (SSS), and functional status (FSS).

Results
Patients had significantly higher BMI and lower vitamin D levels compared to controls (p = 0.003 and p = 0.001, respectively). Those with severe CTS had a significantly higher BMI and lower vitamin D levels than the others (p = 0.03 and p = 0.01 respectively). No significant difference was found between CTS subgroups regarding the SSS, while a higher significant FSS and pain sum score were reported in the severe CTS patients compared to the other two groups (p = 0.01 and p = 0.04 respectively). A significant negative correlation was detected between vitamin D levels and both of BMI, and Boston pain sum scores (p = 0.01 and p = 0.03 respectively). Also, an inverse correlation was detected between vitamin D levels and both of SSS and FSS (p = 0.14, p = 0.06). Furthermore, a significant positive and negative correlation between vitamin D levels and both of conduction velocity and distal motor latency respectively was observed (p = 0.02 and p = 0.01 respectively).

Conclusions
Carpal tunnel syndrome was significantly associated with hypovitaminosis D especially in patients with higher BMI. This highlights the importance of vitamin D supplements and weight loss regimes to minimize the severity of their pain.

Nageeb|Rania S|RS|0000-0002-7598-015X
Shehta|Nahed|N|
Nageeb|Ghada S|GS|
Omran|Alaa A|AA|

Vitamin D (25-dihydroxy cholecalciferol)


Simulating an invasion: unsealed water storage (rainwater tanks) and urban block design facilitate the spread of the dengue fever mosquito, Aedes aegypti, in Brisbane, Australia.

Abstract:
Aedes aegypti (Linnaeus) was once highly prevalent across eastern Australia, resulting in epidemics of dengue fever. Drought conditions have led to a rapid rise in semi-permanent, urban water storage containers called rainwater tanks known to be critical larval habitat for the species. The presence of these larval habitats has increased the risk of establishment of highly urbanised, invasive mosquito vectors such as Ae. aegypti. Here we use a spatially explicit network model to examine the role that unsealed rainwater tanks may play in population connectivity of an Ae. aegypti invasion in suburbs of Brisbane, a major Australian city. We characterise movement between rainwater tanks as a diffusion-like process, limited by a maximum distance of movement, average life expectancy, and a probability that Ae. aegypti will cross wide open spaces such as roads. The simulation model was run against a number of scenarios that examined population spread through the rainwater tank network based on non-compliance rates of tanks (unsealed or sealed) and road grids. We show that Ae. aegypti tank infestation and population spread was greatest in areas of high tank density and road lengths were shortest e.g. cul-de-sacs. Rainwater tank non-compliance rates of over 30% show increased connectivity when compared to less than 10%, suggesting rainwater tanks non-compliance should be maintained under this level to minimize the spread of an invading Ae. aegypti population. These results presented as risk maps of Ae. aegypti spread across Brisbane, can assist health and government authorities on where to optimally target rainwater tank surveillance and educational activities.

Supplementary Information
The online version contains supplementary material available at 10.1007/s10530-021-02619-z.

Trewin|Brendan J|BJ|0000-0002-7244-4044
Parry|Hazel R|HR|
Pagendam|Daniel E|DE|
Devine|Gregor J|GJ|
Zalucki|Myron P|MP|
Darbro|Jonathan M|JM|
Jansen|Cassie C|CC|
Schellhorn|Nancy A|NA|

Water storage
Aedes aegypti
Network model
Population spread
Invasion simulation


Predicting response to cognitive therapy and interpersonal therapy, with or without antidepressant medication, for major depression: a pragmatic trial in routine practice.

Abstract:
BACKGROUND
Identifying patient characteristics that predict response within treatments (prognostic) or between treatments (prescriptive) can inform clinical decision-making. In this study, we sought to identify predictors of response to evidence-based treatments in a sample of depressed patients seeking help in routine practice.

METHODS
Data come from a pragmatic trial of 174 patients with major depression who received an evidence-based treatment of their own choice: cognitive therapy (CT), interpersonal therapy (IPT), antidepressant medication (ADM) alone or in combination with either of the two psychotherapies. Patient characteristics measured at baseline were examined to see if they predicted subsequent response as measured with the Beck Depression Inventory (BDI) over the course of 26 weeks of treatment, using mixed regression modeling.

RESULTS
Higher agoraphobia scores at baseline predicted more change in depression scores across treatments, irrespective of the treatment received. Physical functioning moderated the response to treatment: patients with high physical functioning fared better in combined treatment than patients with low physical functioning, whereas physical functioning did not predict a differential response in the psychotherapy group. Moreover, the lowest levels of physical functioning predicted an increase of depressive symptoms in combined treatment.

LIMITATIONS
A relatively small sample size, and selection of several predictors that were less theory-driven, which hampers the translation to clinical practice.

CONCLUSIONS
If replicated, the prognostic and prescriptive indices identified in this study could guide decision-making in routine practice. Development of more uniform requirements for the analysis and reporting of prediction studies is recommended.

Huibers|Marcus J H|MJ|
van Breukelen|Gerard|G|
Roelofs|Jeffrey|J|
Hollon|Steven D|SD|
Markowitz|John C|JC|
van Os|Jim|J|
Arntz|Arnoud|A|
Peeters|Frenk|F|

D011569:Psychiatric Status Rating Scales

Antidepressant medication
Depression
Evidence-based treatments
Psychotherapy
Prediction of response


Prognostic impact of distinct genetic entities in pediatric diffuse glioma WHO-grade II-Report from the German/Swiss SIOP-LGG 2004 cohort.

Abstract: Reports on pediatric low-grade diffuse glioma WHO-grade II (DG2) suggest an impaired survival rate, but lack conclusive results for genetically defined DG2-entities. We analyzed the natural history, treatment and prognosis of DG2 and investigated which genetically defined sub-entities proved unfavorable for survival. Within the prospectively registered, population-based German/Swiss SIOP-LGG 2004 cohort 100 patients (age 0.8-17.8 years, 4% neurofibromatosis [NF1]) were diagnosed with a DG2. Following biopsy (41%) or variable extent of resection (59%), 65 patients received no adjuvant treatment. Radiologic progression or severe neurologic symptoms prompted chemotherapy (n = 18) or radiotherapy (n = 17). Multiple lines of salvage treatment were necessary for 19/35 patients. Five years event-free survival dropped to 0.44, while 5 years overall survival was 0.90 (median observation time 8.3 years). Extensive genetic profiling of 65/100 DG2 identified Histone3-K27M-mutation in 4, IDH1-mutation in 11, BRAF-V600-mutation in 12, KIAA1549-BRAF-fusions in 6 patients, while the remaining 32 tumor tissues did not show alterations of these genes. Progression to malignant glioma occurred in 12 cases of all genetically defined subgroups within a range of 0.5 to 10.8 years, except for tumors carrying KIAA1549-BRAF-fusions. Histone3-K27M-mutant tumors proved uniformly fatal within 0.6 to 2.4 years. The current LGG treatment strategy seems appropriate for all DG2-entities, with the exemption of Histone3-K27M-mutant tumors that require a HGG-related treatment strategy. Our data confirm the importance to genetically define pediatric low-grade diffuse gliomas for proper treatment decisions and risk assessment.

Falkenstein|Fabian|F|
Gessi|Marco|M|
Kandels|Daniela|D|
Ng|Ho-Keung|HK|
Schmidt|René|R|
Warmuth-Metz|Monika|M|
Bison|Brigitte|B|
Krauss|Juergen|J|
Kortmann|Rolf-Dieter|RD|
Timmermann|Beate|B|
Thomale|Ulrich-Wilhelm|UW|
Albert|Michael H|MH|
Pekrun|Arnulf|A|
Maaß|Eberhard|E|
Gnekow|Astrid K|AK|0000-0002-7356-6887
Pietsch|Torsten|T|

genetics
diffuse glioma WHO-grade II
Histone3 gene mutation
astrocytoma
child


Cytoprotective Compounds Interfere with the Nutraceutical Potential of Bread Supplemented with Green Coffee Beans.

Abstract: The proliferation and motile activity of prostate epithelial (Pnt2) and cancer cells (DU-145; PC-3) in the presence of bioavailable compounds from green coffee beans (GCB), wholemeal wheat bread (WMWB), and its GCB-fortified variant were analyzed. The considerable cytostatic and anti-invasive activity of GCB extracts was correlated with its phenolic contents. WMWB extract contained significantly lower levels of phenolics but still displayed relatively high cytostatic activity. However, the cytostatic properties of WMWB compounds were hardly augmented by 3% GCB flour supplementation. The cytoprotective activity of the WMWB compounds exerts a negative impact on the cytostatic activity of GCB compounds. These data confirm the relatively high chemopreventive potential of GCB. However, they also indicate that subtle interactions between bioavailable compounds in GCB and WMWB can negatively affect the nutraceutic potential of the fortified bread. Apparently, gastrointestinal processing differentially regulates the availability of individual compounds and affects the balance between the cytostatic and cytoprotective activity of the whole product. Our data show that comprehensive research is necessary before the fortification of a specific carrier with a specific supplement can be recommended.

cytoprotection
coffee beans
gastrointestinal digestion
prostate cancer
wholemeal bread


The discrimination threshold: A selection criterion for analytical methods based on measurement uncertainty - Application to animal stress studies.

Abstract: Many biological studies seek to confirm the effect of a treatment on the levels of chemical markers in biological tissues. This requires choosing the analytical method best able to detect the difference between basal levels and those found after treatment. We propose a new approach to calculate a criterion we call the 'discrimination threshold' (DT), and we applied it to an analytical method that we developed to determine cortisol in cattle plasma to detect stress. DT is derived from the measurement uncertainty (MU), and combines the variabilities of both the analytical method and the marker. The uncertainty of the analytical method comes from the method validation study. The marker variability (here cortisol) is modeled from a literature review. The graphical representation of DT allows estimating the applicability of the method. Our analytical method is shown to perform well when the difference in blood cortisol exceeds 18 ng. mL-1.

Andanson|Stephane|S|
Veissier|Isabelle|I|
Feinberg|Max H|MH|

Discrimination threshold
Measurement uncertainty
Accuracy profile
Bovine cortisol
ELISA test
Selection criteria


Odontocete peduncle tendons for possible control of fluke orientation and flexibility.

Abstract: Dorso-ventral oscillations of cetacean caudal flukes generate lift-based thrust for swimming. Movements of the flukes are actuated by epaxial and hypaxial muscles through caudal tendons inserting onto vertebrate in the peduncle. To determine if the caudal tendons in the peduncle affect the flexibility of the flukes, we must understand how the tendons from axial muscles insert onto the caudal vertebrate. The purpose of this study was to provide a detailed description of the various tendons within the cetacean peduncle with regard to their role in swimming and flexibility. Dissection of the peduncle and flukes of multiple odontocete species showed that there were two distinct epaxial tendon sets within the peduncle: (1) extensor caudae medialis tendon (ECM) and (2) extensor caudae lateralis tendon (ECL). There is one distinct hypaxial tendon set, the medial tendon of the hypaxialis lumborum (MHL). The ECM and MHL tendons inserted serially onto caudal vertebrae while the ECL inserted exclusively onto the terminal vertebrae posterior to the fluke insertion. It is typical that tendons insert onto bone, however, the connection to the core fibrous layer of the flukes suggests an element of active control of the flexibility of the flukes via the axial muscles. Tension from muscular contraction transmitted through the tendons could affect both spanwise and chordwise flexibility. Changing flexibility could modulate thrust and efficiency over an extended operation range of swimming speeds in cetaceans.

flexibility vertebrae dolphin locomotion porpoise propulsion stiffness


Management of portal vein thrombosis in cirrhotic patients enlisted for liver transplantation: From diagnosis to treatment (Review).

Abstract: Portal vein thrombosis (PVT) commonly occurs in patients with cirrhosis. Several classification systems of PVT have been proposed over the years reflecting the challenge in establishing a widely accepted system. To date, PVT has been considered an absolute contraindication for orthotopic liver transplantation (OLT) since it is associated with decreased graft survival and increased patient mortality. Nevertheless, the development of modern surgical techniques has enabled the inclusion of these candidates in OLT waiting lists, since their postoperative results are shown to be comparable to those of patients without PVT. The aim of the present review was to critically appraise the available treatment options for cirrhotic patients enlisted for liver transplantation in the setting of PVT.

Kotsifa|Evgenia|E|
Kykalos|Stylianos|S|
Machairas|Nikolaos|N|
Nikiteas|Nikolaos|N|
Sotiropoulos|Georgios C|GC|

portal vein
thrombosis
liver transplantation
cirrhosis
end stage liver disease


A multi-level developmental approach towards understanding adolescent mental health and behaviour: rationale, design and methods of the LIFECOURSE study in Iceland.

Abstract:
PURPOSE
Identifying and understanding modifiable risk and protective factors that can inform early detection and intervention to prevent adolescent emotional problems and harmful behaviours is among the most pressing modern-day public health challenges. This paper describes the rationale, objectives, methods, and anticipated outcomes of the LIFECOURSE study, a multi-level, bio-psychosocial prospective study designed to advance our understanding of factors that shape adolescent mental health and behaviour.

METHODS
Conducted by the Icelandic Centre for Social Research and Analysis at Reykjavik University, LIFECOURSE is a longitudinal population-based developmental study of Icelandic adolescents born in 2004. The study utilizes a comprehensive multi-informant assessment of individual, societal and biological factors measured across the lifespan. Data assembly and collection were conducted from 2016-2020 and utilize both retrospective and prospective data sources: (a) retrospective registry data assembled from seven national databases, (b) prospectively collected social surveys and (c) biomarker samples.

RESULTS
Of the 3914 eligible adolescents, 60.8% (n = 2378) provided informed parental consent and student assent to participate in the study, with approximately half of the participants being female (n = 1175, 49.4%) and the majority being born in the capital area (n = 1455; 61.2%). The coverage of available data from the national databases and participation in the social surveys ranged from 81.7 to 100%.

CONCLUSIONS
Major gaps remain in our knowledge of how individual, societal and biological factors across the lifespan-from early life to adolescence-interact and shape the risk for emotional problems and harmful behaviours during adolescence. The LIFECOURSE study was designed to address this knowledge gap.

Adolescence
Emotional problems
Bio-psychosocial model
Delinquency
Stress
Substance abuse


Effect of normobaric hypoxic exercise on blood pressure in old individuals.

Abstract:
PURPOSE
To test the hypothesis that the combination of endurance training and hypoxia leads to greater improvements in resting and exercise blood pressure in old sedentary individuals compared to endurance training only.

METHODS
We randomly assigned 29 old overweight participants (age: 62 ± 6 years, body mass index (BMI): 28.5 ± 0.5 kg/m2, 52% men) to single blind 8-week bicycle exercise in hypoxia (fraction of inspired oxygen (FIO2) = 0.15) or normoxia (FIO2 = 0.21). Brachial blood pressure was measured at rest, during maximal incremental exercise testing, and during a 30 min constant work rate test, at baseline and after the training period.

RESULTS
Work rate, heart rate and perceived exertion during training were similar in both groups, with lower oxygen saturation for participants exercising under hypoxia (88.7 ± 1.5 vs. 96.2 ± 1.2%, t(27) = - 13.04, p < 0.001, |g|= 4.85). Office blood pressure and blood pressure during incremental exercise tests did not change significantly in either group after the training program. Systolic blood pressure during the constant work rate test was reduced after training in hypoxia (160 ± 18 vs. 151 ± 14 mmHg, t(13) = 2.44 p < 0.05, |d|= 0.55) but not normoxia (154 ± 22 vs. 150 ± 16 mmHg, t(14) = 0.75, p = 0.46, |d|= 0.18) with no difference between groups over time (F = 0.08, p = 0.77, η2 = 0.01).

CONCLUSION
In old individuals hypoxia in addition to exercise does not have superior effects on office or exercise blood pressure compared to training in normoxia.

TRIAL REGISTRATION NUMBER
ClinicalTrials.gov No. NCT02196623 (registered 22 July 2014).


Sphingosine inhibits the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) activity.

Abstract: The increase in the intracellular Ca(2+) concentration ([Ca(2+)]i) is the key variable for many different processes, ranging from regulation of cell proliferation to apoptosis. In this work we demonstrated that the sphingolipid sphingosine (Sph) increases the [Ca(2+)]i by inhibiting the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA), in a similar manner to thapsigargin (Tg), a specific inhibitor of this Ca(2+) pump. The results showed that addition of sphingosine produced a release of Ca(2+) from the endoplasmic reticulum followed by a Ca(2+) entrance from the outside mileu. The results presented in this work support that this sphingolipid could control the activity of the SERCA, and hence sphingosine may participate in the regulation of [Ca(2+)]I in mammalian cells.

Benaim|Gustavo|G|
Pimentel|Adriana A|AA|
Felibertt|Pimali|P|
Mayora|Adriana|A|
Colman|Laura|L|
Sojo|Felipe|F|
Rojas|Héctor|H|
De Sanctis|Juan B|JB|

D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases


Giant peritoneal loose body: A case report.

Abstract: Peritoneal loose body (PLB) is a rare finding and it is usually incidentally discovered during laparotomy, examination or autopsy, as it is usually asymptomatic or presents with non-specific symptoms. In particular, giant PLBs, measuring >50 mm in maximum diameter, are even more rare, and only a few cases of laparoscopic extraction of these giant bodies have been reported in the literature to date. We herein describe our experience with the diagnosis and treatment of a giant PLB in a 49-year-old male patient who was admitted with complaints of intermittent pain the in lower abdomen 3 months earlier. An abdominal computed tomography scan revealed a 5.5x5.0-cm midline mass with central high density. The diagnosis was confirmed by open surgery and postoperative pathological examination. The postoperative recovery of the patient was uneventful and he was discharged from the hospital 7 days after surgery.

Guo|Shanshan|S| Yuan|Haijuan|H| Xu|Yingying|Y| Chen|Ping|P| Zong|Liang|L|

peritoneal loose body
case report
appendices epiploicae


Covariates of the Severity of Aggression in Sexual Crimes: Psychopathy and Borderline Characteristics.

Abstract: Whereas risk assessment literature on sexual offending has primarily focused on prediction of subsequent sexual crimes, and not the severity of those crimes, the first aim of the present study was to identify variables that predict the amount of damage to victims in sexual crimes compared with those that predict general aggressiveness. The second aim was to ascertain whether adding emotional instability measurements, as in borderline personality disorder (BPD), would add incremental variance to that captured by the facets of the Psychopathy Checklist-Revised (PCL-R). Trained raters assessed on the PCL-R, BPD, and measures of severity of sexual and nonsexual violence 302 adults who had sexually offended. PCL-R's Antisociality and two externalizing BPD factors (one from the standard and one from the alternative criteria) were significant predictors of violence both in sexual and nonsexual crimes. In contrast, deficits in the PCL-R's Affective facet (2) predicted victim damage in sexual contexts only, whereas the Lifestyle Impulsivity facet (3) of the PCL-R predicted violence in nonsexual contexts only. These findings suggest that adding measures of emotional dysregulation to commonly used instruments like the PCL-R, which assesses callousness and antisociality, may be beneficial for predicting violence.

Cardona|Nicole|N|
Berman|Ariel K|AK|
Sims-Knight|Judith E|JE|
Knight|Raymond A|RA|

psychopathy
risk assessment
sexual offending
borderline personality disorder
personality disorder
child molestation
child molesters
dangerousness
rape
rapists
sex offenses


Cold stress in maize (Zea mays) is alleviated by the over-expression of Phytoglobin 1 (ZmPgb1.1).

Abstract: Maize (Zea mays) plants over-expressing or suppressing the class 1 Phytoglobin (ZmPgb1.1) were evaluated for their ability to cope with low temperature stress. Cold treatment (10 °C day/4 °C night) depressed several gas exchange parameters including photosynthetic rate, stomatal conductance and transpiration, while elevated the levels of reactive oxygen species (ROS) and ROS-induced damage. These effects were attenuated by the over-expression of ZmPgb1.1, and aggravated when the level of the same gene was suppressed. Combination of transcriptomic and pharmacological studies revealed that over-expression of ZmPgb1.1 suppressed the level of nitric oxide (NO), which lowers the transcription of several Brassinosteroid (BR) biosynthetic and response genes. Cellular BR was required to induce the expression of ZmMPK5, a component of the mitogen-activated protein kinase (MAPK) cascade, which is known to be involved in the regulation of ROS-producing pathways. Experimental reduction of NO content, suppression of BR or inhibition of ZmMPK5 reverted the beneficial effects of ZmPgb1.1 over-expression, and increased plant susceptibility to cold stress through accumulation of ROS. Conversely, tolerance to cold was augmented in the ZmPgb1.1 down-regulating line when the levels of NO or BR were elevated. Together, this study demonstrates a novel role of ZmPgb1.1 in modulating plant performance to cold stress, and integrates the ZmPgb1.1 response in a model requiring NO and BR to alleviate oxidative stress through ZmMPK5.

Mira|Mohamed M|MM|
Ibrahim|Shimaa|S|
Hill|Robert D|RD|
Stasolla|Claudio|C|

D018506:Gene Expression Regulation, Plant

Cold stress
Phytoglobin
Reactive oxygen species
Nitric oxide
Brassinosteroids


Use of Pressurized Air Infusion For Pre Descemet's Endothelial Keratoplasty (PDEK) - The Air Pump Assisted PDEK Technique.

Abstract:
Purpose
To assess the advantages offered by the air pump assisted PDEK technique that utilizes pressurized Anterior Chamber (AC) air infusion.

Methods
Pressurized air infusion was provided through an anterior chamber maintainer connected to the fluid air exchange system of a posterior vitrectomy machine during surgery.

Results
Pressurized air infusion within the AC helped perform Descemetorhexis, prevented bleeding during Peripheral Iridectomy (PI) and synechiolysis, prevented oozing of blood from peripheral corneal neovascularization into the AC and thus helped maintain a non-fibrinous AC environment. In addition, it helped in precise graft manipulation, centration, edge unfolding and unwrinkling after it was floated against the stroma as well as faster graft adhesion. It also prevented AC depth fluctuations during intra-cameral maneuvers and prevented intra-operative as well as post-operative graft detachment.

Conclusions
This technique makes several steps of surgery easier and improves graft adhesion.


Rapid Fabrication of Superhydrophobic Virtual Walls for Microfluidic Gas Extraction and Sensing.

Abstract: Based on the virtual walls concept, where fluids are guided by wettability, we demonstrate the application of a gas phase extraction microfluidic chip. Unlike in previous work, the chip is prepared using a simple, rapid, and low-cost fabrication method. Channels were cut into double-sided adhesive tape (280 µm thick) and bonded to hydrophilic glass slides. The tape was selectively made superhydrophobic by 'dusting' with hydrophobic silica gel to enhance the wettability contrast at the virtual walls. Finally, the two glass slides were bonded using tape, which acts as a spacer for gas transport from/to the guided liquids. In our example, the virtual walls create a stable liquid-vapor-liquid flow configuration for the extraction of a volatile analyte (ammonia), from one liquid stream to the other through the intermediate vapor phase. The collector stream contained a pH indicator to visualize the mass transport. Quantitative analysis of ammonium hydroxide in the sample stream (<1 mM) was possible using a characteristic onset time, where the first pH change in the collector stream was detected. The effect of gap length, flow rates, and pH of the collector stream on the onset time is demonstrated. Finally, we demonstrate the analysis of ammonium hydroxide in artificial human saliva to show that the virtual walls chip is suitable for extracting volatile analytes from biofluids.

virtual walls
gas extraction
ammonia
Laplace pressure
microfabrication


Disease knowledge and attitudes during the COVID-19 epidemic among international migrants in China: a national cross-sectional study.

Abstract: Background: There are more than 258 million international migrants worldwide and the majority reside in countries with ongoing novel coronavirus disease 2019 (COVID-19) epidemic outbreaks. International migrants may not receive adequate and timely disease information during epidemics, increasing vulnerability to disease transmission. This is one of very limited studies focusing on international migrants' COVID-19 prevention knowledge and attitudes during the epidemic. Methods: A national cross-sectional online survey was conducted across 100 cities and 26 regions in China from February 17 and March 1, 2020. The sample included 1,426 international migrants representing 77 countries and 6 continents. Knowledge was defined as the number of correct responses to questions about COVID-19. Attitudes included worries, expectations, and general preparedness. Multivariable ordinal logistic regressions evaluated correlates of knowledge and attitudes including information channels and preferences, and trust in Chinese institutions and groups. Results: Just half of the sample, 730/1426 (51.2%) had a good level of knowledge and 656/1426 (46.0%) had a positive attitude towards the COVID-19 epidemic. Knowledge was associated with receiving information through social media (aOR: 2.0, 95%CI: 1.2-3.2), the Internet (aOR: 1.4, 95%CI: 1.2-1.8), the community (aOR: 1.5, 95%CI: 1.2-1.8), and encountering language barriers when receiving medical services (aOR: 0.8, 95%CI: 0.7-1.0). Positive attitude was associated with the level of trust in various Chinese institutions and groups. Conclusions: Roughly half of the sample reported inadequate knowledge and poor attitudes toward prevention and control of COVID-19. Tailored public health campaigns are needed to ensure that international migrants possess adequate knowledge to protect their health during future epidemics and disasters.

Wang|Cheng|C|
Tian|Qi|Q|
Zhao|Peizhen|P|
Xiong|Mingzhou|M|
Latkin|Carl A|CA|
Gan|Yiqun|Y|
Hall|Brian J|BJ|
Yang|Bin|B|

D007722:Health Knowledge, Attitudes, Practice

knowledge
attitude
COVID-19
international migrants


Towards understanding the molecular recognition process in prokaryotic zinc-finger domain.

Abstract: Eukaryotic Cys2His2 zinc finger domain is one of the most common and important structural motifs involved in protein-DNA interaction. The recognition motif is characterized by the tetrahedral coordination of a zinc ion by conserved cysteine and histidine residues. We have characterized the prokaryotic Cys2His2 zinc finger motif, included in the DNA binding region (Ros87) of Ros protein from Agrobacterium tumefaciens, demonstrating that, although possessing a similar zinc coordination sphere, this domain presents significant differences from its eukaryotic counterpart. Furthermore, basic residues flanking the zinc binding region on either side have been demonstrated, by Electrophoretic Mobility Shift Assay (EMSA) experiments, to be essential for Ros DNA binding. In spite of this wealth of knowledge, the structural details of the mechanism through which the prokaryotic zinc fingers recognize their target genes are still unclear. Here, to gain insights into the molecular DNA recognition process of prokaryotic zinc finger domains we applied a strategy in which we performed molecular docking studies using a combination of Nuclear Magnetic Resonance (NMR) and Molecular Dynamics (MD) simulations data. The results demonstrate that the MD ensemble provides a reasonable picture of Ros87 backbone dynamics in solution. The Ros87-DNA model indicates that the interaction involves the first two residue of the first α-helix, and several residues located in the basic regions flanking the zinc finger domain. Interestingly, the prokaryotic zinc finger domain, mainly with the C-terminal tail that is wrapped around the DNA, binds a more extended recognition site than the eukaryotic counterpart. Our analysis demonstrates that the introduction of the protein flexibility in docking studies can improve, in terms of accuracy, the quality of the obtained models and could be particularly useful for protein showing high conformational heterogeneity as well as for computational drug design applications.

Russo|Luigi|L|
Palmieri|Maddalena|M|
Caso|Jolanda Valentina|JV|
D'Abrosca|Gianluca|G|
Diana|Donatella|D|
Malgieri|Gaetano|G|
Baglivo|Ilaria|I|
Isernia|Carla|C|
Pedone|Paolo V|PV|
Fattorusso|Roberto|R|

D024202:Electrophoretic Mobility Shift Assay

Prokaryotic zinc finger
Docking
NMR
Molecular Dynamics simulations
Protein-DNA complex


Personalising lung cancer screening: An overview of risk-stratification opportunities and challenges.

Abstract: Randomised clinical trials have shown the efficacy of computed tomography lung cancer screening, initiating discussions on whether and how to implement population-based screening programs. Due to smoking behaviour being the primary risk-factor for lung cancer and part of the criteria for determining screening eligibility, lung cancer screening is inherently risk-based. In fact, the selection of high-risk individuals has been shown to be essential in implementing lung cancer screening in a cost-effective manner. Furthermore, studies have shown that further risk-stratification may improve screening efficiency, allow personalisation of the screening interval and reduce health disparities. However, implementing risk-based lung cancer screening programs also requires overcoming a number of challenges. There are indications that risk-based approaches can negatively influence the trade-off between individual benefits and harms if not applied thoughtfully. Large-scale implementation of targeted, risk-based screening programs has been limited thus far. Consequently, questions remain on how to efficiently identify and invite high-risk individuals from the general population. Finally, while risk-based approaches may increase screening program efficiency, efficiency should be balanced with the overall impact of the screening program. In this review, we will address the opportunities and challenges in applying risk-stratification in different aspects of lung cancer screening programs, as well as the balance between screening program efficiency and impact.

D016032:Randomized Controlled Trials as Topic

lung cancer screening
risk-based screening
personalised screening


Periodic microstructures of blood capillaries revealed by synchrotron X-ray multi-resolution microscopic analysis.

Abstract: Cardiovascular diseases are closely related to structural blood capillaries lesions. Herein, microscopic investigations of mouse blood capillaries were performed at multiple spatial resolution by using synchrotron X-ray in-line phase contrast tomography and scanning transmission X-ray microscopy (STXM). The chemically fixed blood capillaries without any contrast agents were selected. For the first time, a periodic bamboo-shaped structure was observed at nanoscale resolution by STXM, and the three-dimensional tomographic slices at sub-micrometer resolution further confirmed the periodic wave profile of the blood capillaries. Then, a periodic microstructural model was suggested based on the microscopic images. By using high-performance imaging techniques, this work provides a better understanding of the relationship between the structure and function of blood capillaries, will be helpful in elucidating the causes of cardiovascular system diseases.

Yao|Shengkun|S| Zong|Yunbing|Y| Huang|Xu|X| Liu|Yang|Y| Gong|Ningqiang|N| Zhang|Jianhua|J| Li|Ziqing|Z| Cao|Feng|F| Wang|Xiangcheng|X| Liang|Xing-Jie|XJ| Jiang|Huaidong|H|

(170.3880) Medical and biological imaging


Room for Improvement: The HIV-Diabetes Care Continuum Over 15 Years in the Women's Interagency HIV Study.

Abstract:
Background
Gains in life expectancy through optimal control of HIV infection with antiretroviral therapy (ART) may be threatened if other comorbidities, such as diabetes, are not optimally managed.

Methods
We analyzed cross-sectional data of the Women's Interagency HIV Study (WIHS) from 2001, 2006, and 2015. We estimated the proportions of HIV-positive and HIV-negative women with diabetes who were engaged in care and achieved treatment goals (hemoglobin A1c [A1c] <7.0%, blood pressure [BP] <140/90 mmHg, low-density lipoprotein [LDL] cholesterol <100 mg/dL, not smoking) and viral suppression. Repeated-measures models were used to estimate the adjusted prevalence of achieving each diabetes treatment goal at each time point, by HIV status.

Results
We included 486 HIV-positive and 258 HIV-negative women with diabetes. In 2001, 91.8% visited a health care provider, 60.7% achieved the A1c target, 70.5% achieved the BP target, 38.5% achieved the LDL cholesterol target, 49.2% were nonsmokers, 23.3% achieved combined ABC targets (A1c, BP, and cholesterol), and 10.9% met combined ABC targets and did not smoke. There were no differences by HIV status, and patterns were similar in 2006 and 2015. Among HIV-positive women, viral suppression increased from 41% in 2001 to 87% in 2015 compared with 8% and 13% achieving the ABC goals and not smoking. Viral suppression was not associated with achievement of diabetes care goals.

Conclusions
Successful management of HIV is outpacing that of diabetes. Future studies are needed to identify factors associated with gaps in the HIV-diabetes care continuum and design interventions to better integrate effective diabetes management into HIV care.

Colasanti|Jonathan|J|
Galaviz|Karla I|KI|
Christina Mehta|C|C|
Palar|Kartika|K|
Schneider|Michael F|MF|
Tien|Phyllis|P|
Adimora|Adaora A|AA|
Alcaide|Maria|M|
Cohen|Mardge H|MH|
Gustafson|Deborah|D|
Karim|Roksana|R|
Merenstein|Daniel|D|
Sharma|Anjali|A|
Wingood|Gina|G|
Marconi|Vincent C|VC|
Ofotokun|Ighovwerha|I|
Ali|Mohammed K|MK|


Air pollution from natural and anthropic sources and male fertility.

Abstract: Exposure to air pollution has been clearly associated with a range of adverse health effects, including reproductive toxicity. However, a limited amount of research has been conducted to examine the association between air pollution and male reproductive outcomes, specially semen quality. We performed a systematic review (up to March 2017) to assess the impact of environmental and occupational exposure to air pollution on semen quality. Epidemiological studies focusing on air pollution exposures and male reproduction were identified by a search of the PUBMED, MEDLINE, EBSCO and TOXNET literature bases. Twenty-two studies were included which assess the impact of air pollutants (PM2.5, PM10, SO2, NOx, O3, PAHs) on main semen parameters (sperm concentration, motility, morphology), CASA parameters, DNA fragmentation, sperm aneuploidy and the level of reproductive hormones. The number of studies found significant results supporting the evidence that air pollution may affect: DNA fragmentation, morphology and motility.In summary, most studies concluded that outdoor air pollution affects at least one of the assessed semen parameters. However the diversity of air pollutants and semen parameters presented in the studies included in the review and different study design caused lack of consistency in results and difficulties in comparison.

Jurewicz|Joanna|J| Dziewirska|Emila|E| Radwan|Michał|M| Hanke|Wojciech|W|

Air pollution
Male fertility
Semen quality
Occupational exposure
Environmental exposure


A critical literature review on the use of bellyboard devices to control small bowel dose for pelvic radiotherapy.

Abstract: Delivering curative radiotherapy doses for rectal and gynaecological tumours has historically been complicated by the dose tolerance of the small bowel. Acute radiation-induced small bowel toxicity includes side effects such as abdominal pain, nausea and diarrhoea. With the advent of new treatment delivery modalities, such as IMRT (Intensity modulated radiotherapy) and VMAT (Volumetric modulated Arc radiotherapy), there has been an expectation that small bowel doses can be better controlled with the use of these technologies. These capabilities enable the creation of treatment plans that can better avoid critical radiosensitive organs. The purpose of this review is to look beyond advances in linear accelerator technology in seeking improvements to small bowel dose and toxicity. This review examines whether an alternative prone patient positioning approach using a bellyboard device in conjunction with IMRT and VMAT treatment delivery can reduce small bowel doses further than using these technologies with the patient in a traditional supine position.

Hoffmann|Matthew|M| Waller|Kim|K| Last|Andrew|A| Westhuyzen|Justin|J|

Bellyboard
Small bowel
Supine position
Gynaecological radiotherapy
Pelvis
Prone position
Rectum


Anatomical risk factors for spermatic cord torsion and their involvement in the choice of orchidopexy technique.

Abstract:
OBJECTIVE
We aim by this article to present a literature review on the anatomical risk factors for spermatic cord torsion and their involvement the different techniques of orchidopexy.

MATERIALS AND METHODS
The literature review was conducted following PRISMA check-list, using PubMed database and the Mesh terms: testis, torsion, anomaly, orchidopexy. Techniques of surgical management provided by the EMC (French medico-surgical encyclopedia) were also used.

RESULTS
Database research identified 500 articles, and 16 mores articles were added from the review of the references lists of relevant articles. At the end, 42 articles were useful for our review including 6 reviews, 5 cross-sectional studies, 14 cohorts, 10 case reports, 1 meta-analysis, 1 letter, and 5 experimental studies. High insertion of the tunica vaginalis, a long mesorchium, deficient attachment of the epididymis to the testis, and anomalies of the gubernaculum testis are the anatomical predispositions for spermatic cord torsion. After reduction of torsion, many surgical techniques for the fixation of the testis have been suggested including: conventional technique by triangulation with or without fasciotomy, window orchidopexy, scrotal pouch, and vaginal eversion with or without dartos fixation.

CONCLUSION
The most common anatomical factor associated with spermatic cord torsion is the high insertion of the tunica vaginalis. Vaginal eversion with fixation to the dartos might be superior to other known techniques and seems promising to become standard.

Spermatic cord
Torsion
Orchidopexy
Testis
Fixation
Anatomy


Correlation between Plasma Adiponectin Levels and the Presence and Severity of Coronary Artery Disease.

Abstract:
BACKGROUND
The existing evidence suggests that plasma adiponectin concentrations can be indicative of the presence and severity of coronary artery disease (CAD). However, the results of the studies conducted hitherto on this subject are inconsistent. We sought to investigate the possible correlation between plasma adiponectin levels and the presence and severity of CAD in patients undergoing non-urgent coronary angiography.

METHODS
In 399 consecutive patients undergoing non-urgent coronary angiography for CAD survey, plasma adiponectin, triglyceride, total cholesterol, high-density lipoprotein and low-density lipoprotein cholesterol, and fasting blood sugar levels were measured and demographic characteristics such as age, sex, Body Mass Index, diabetes mellitus history, systemic hypertension history, and family history of CAD were collected. According to the angiography results, the patients were divided into two groups of CAD and non-CAD. The severity of coronary atherosclerosis in the CAD group was defined using the Gensini score system.

RESULTS
Average age was 61.4 ± 9.94 years in the CAD group and 57.9 ± 10.75 years in the non-CAD group. Also, 73.7% of the CAD group and 55.4% of the non-CAD group were male. Totally, 278 (69.7%) patients were found to have CAD. Patients without CAD did not have higher mean plasma adiponectin concentrations than did those with CAD (13.38 ± 11.96 vs. 14.95 ± 14.11 mcg/ml; p value = 0. 896). After adjustment for CAD conventional risk factors, plasma adiponectin levels still were not associated with CAD. No association was found between plasma adiponectin levels and the Gensini score. Furthermore, in contrast to the fairly strong correlation previously reported, there was no correlation between adiponectin levels and conventional CAD risk factors.

CONCLUSION
We could not observe any relationship between plasma adiponectin concentrations and the presence or severity of CAD in patients undergoing coronary angiography.

Amirzadegan|Alireza|A|
Shakarami|Amir|A|
Borumand|Mohammad Ali|MA|
Davoodi|Gholamreza|G|
Ghaffari-Marandi|Neda|N|
Jalali|Arash|A|

Adiponectin
Coronary artery disease
Coronary angiography
Risk factors


Seasonal dynamics alter taxonomical and functional microbial profiles in Pampa biome soils under natural grasslands.

Abstract: Soil microbial communities' assembly is strongly tied to changes in temperature and moisture. Although microbial functional redundancy seems to overcome taxonomical composition changes, the sensitivity and resilience of soil microbial communities from subtropical regions in response to seasonal variations are still poorly understood. Thus, the development of new strategies for biodiversity conservation and sustainable management require a complete understanding of the soil abiotic process involved in the selection of microbial taxa and functions. In this work, we used state of the art molecular methodologies (Next Generation Sequencing) to compare the taxonomic (metataxonomics) and functional (metatranscriptomics) profiles among soil samples from two subtropical natural grasslands located in the Pampa biome, Brazil, in response to short-term seasonal variations. Our data suggest that grasslands maintained a stable microbial community membership along the year with oscillation in abundance. Apparently soil microbial taxa are more susceptible to natural climatic disturbances while functions are more stable and change with less intensity along the year. Finally, our data allow us to conclude that the most abundant microbial groups and functions were shared between seasons and locations reflecting the existence of a stable taxonomical and functional core microbiota.

Barboza|Anthony Diego Muller|ADM|
Pylro|Victor Satler|VS|
Jacques|Rodrigo Josemar Seminot|RJS|
Gubiani|Paulo Ivonir|PI|
de Quadros|Fernando Luiz Ferreira|FLF|
da Trindade|Júlio Kuhn|JK|
Triplett|Eric W|EW|
Roesch|Luiz|L|

Seasons
Metataxonomics
Metatranscriptomics
16S rRNA gene
Abiotic factors
Ion Torrent PGM
NGS
Soil microbial core
Subtropical ecosystems


Quantification of the Effect of the Cattle Breed on Milk Cheese Yield: Comparison between Italian Brown Swiss and Italian Friesian.

Abstract: Milk from different cattle breeds can present different casein and fat contents, which are reflected in different cheese yields (CY). However, CY is also related to some breed-related molecular characteristics. The aim of the present work was to quantify the effect of these characteristics by comparing a series of Parmigiano Reggiano (PR) cheese-making trials made with milks from Italian Brown (IB) and Italian Friesian (IF) cattle herds. Twelve trials were carried out in a cheese factory in one year (one trial per month), each one consisting of four vats processed in parallel: three vats contained milk from three different IF cattle herds (IF1, IF2 and IF3) and one contained milk from a single IB cattle herd. A 24-h CY prediction formula was developed with data from IF1, IF2 and IF3 trials (calibration) and successively validated by applying it to 12 PR trials made with IF milk in six different cheese factories (external validation). The predicted values of 24-h CY were no different to the actual ones in both calibration and external validation. Finally, the formula was tested on trials made with IB milk. In this case, the predicted values were lower than the actual ones. The quantity of IF milk casein necessary to give the same CY of IB milk was 0.20 g/100 g.

Parmigiano Reggiano cheese
cheese yield prediction formula
k-casein B
multiple regression analysis


Polymorphism of Transcription Factor-7-Like 2 (TCF7L2) Gene and New-Onset Diabetes after Liver Transplantation.

Abstract:
BACKGROUND
New-onset diabetes after transplantation (NODAT) is a serious complication in transplant recipients. Transcription factor-7-like 2 (TCF7L2) is a Wnt signaling-associated transcription factor that plays an important role in β-cell proliferation and insulin secretion. The association between TCF7L2 SNP rs7903146 and NODAT was documented in renal transplant patients.

OBJECTIVE
To determine the association between TCF7L2 rs7903146 variants and the risk of NODAT after liver transplantation.

METHODS
This study was conducted on 140 liver transplant recipients who had received tacrolimus-based immunosuppressive drugs. The patients were divided into NODAT (n=70) and non-NODAT (n=70) groups and were genotyped using PCR-RFLP. In addition, 100 normal subjects were considered as the comparison group.

RESULTS
There was a significant difference (p<0.05) between the two study groups regarding donor and recipient age, recipient body mass index, and recipient fasting plasma glucose before the transplantation. No significant relationship was observed between TCF7L2 rs7903146 genotypes and development of NODAT. No significant difference was also found between the two groups in terms of the tacrolimus and mycophenolate mofetil daily dosage as well as tacrolimus blood level. However, the prednisolone daily dosage was significantly (p=0.01) higher in the NODAT group compared to those without NODAT. The majority of the patients in the NODAT group also had an episode of acute rejection. Furthermore, a significant difference was found between the transplant recipients and the comparison subjects regarding T allele (p<0.001, OR=1.96) and TT genotype (p<0.001, OR=3.47) frequencies.

CONCLUSION
No correlation was found between TCF7L2 genotypes and development of NODAT. Acute rejection and prednisolone pulse therapy predisposed the susceptible patients to NODAT.

Musavi|Z|Z|
Azarpira|N|N|
Sangtarash|M H|MH|
Kordi|M|M|
Kazemi|K|K|
Geramizadeh|B|B|
Malek-Hosseini|S A|SA|

Polymorphism
Liver transplantation
Transcription factors
Diabetes mellitus
Transcription factor 7-like 2 protein
genetic


A Longitudinal Case Study: The Development of Exceptional Human Experiences of Senior Ecclesiastical Professionals in the Catholic Church.

Abstract: Exceptional human experiences (EHEs) impact on health and well-being and can contribute to enhanced intercultural and interreligious awareness and understanding. The aim of this longitudinal study was to explore the development of EHEs in a group of senior professionals in the German Catholic Church. Exceptional human experiences were measured through the EEQ in pre- and post-test questionnaires which were qualitatively analysed. The results of this study reflect an increase in the frequency of positive spiritual experiences and visionary dream encounters, as well as a more positive evaluation of these spiritual phenomena. The findings seem to suggest that it is possible to raise people's awareness of spiritual practices and to enhance intercultural and interreligious competence through training interventions.

Mayer|Claude-Hélène|CH| Viviers|Rian|R| Flotman|Aden-Paul|AP| Schneider-Stengel|Detlef|D|

Exceptional human experiences
Interculture
German Catholic Church
Spirituality
Training


Building an inclusive research team: the importance of team building and skills training.

Abstract:
BACKGROUND
Inclusive research teams typically describe their experiences and analyse the type of involvement of researchers with disability, but the process of building research teams and the need for research training still remain underexplored in the literature.

MATERIALS AND METHOD
Four researchers with intellectual disabilities and four academic researchers developed an inclusive research team. The team conducted 15 research training sessions, focused on investigating the well-being of older women with intellectual disabilities. They used mobile technology to support research skills acquisition.

RESULTS
Findings included the experiences of all team members regarding the team building during training.

CONCLUSIONS
To become an effective inclusive research team, all team members, regardless of ability, need to bring their own experiences and also learn necessary research skills. This paper highlights the need for team building, joint research training among all members of the research team and strategies supporting the peer-mentoring within the team. We are a team of four researchers with intellectual disabilities and four academic researchers without an intellectual disability. Our aim has been to learn about research together. We want to do this so that we can carry out a research project together about how older women with intellectual disabilities live. We have decided to call our team 'Welcome to our Class'. We have been working together for 9 months. In this time we have had 15 research training meetings. We have learned What research is How to work out a research question, that is what we want to find out about How to get information on what we want to find out. Here we thought of interview questions we could ask older women with intellectual disabilities. We are now meeting once a month, and have just begun our research on finding out how older women with intellectual disabilities live. We are now starting to use what we have learned.

Strnadová|Iva|I|
Cumming|Therese M|TM|
Knox|Marie|M|
Parmenter|Trevor|T|

inclusive research
research training
intellectual disability
research skills
peer-mentoring
participatory research


WNT-5A regulates TGF-β-related activities in liver fibrosis.

Abstract: WNT-5A is a secreted growth factor that belongs to the noncanonical members of the Wingless-related MMTV-integration family. Previous studies pointed to a connection between WNT-5A and the fibrogenic factor TGF-β warranting further studies into the functional role of WNT-5A in liver fibrosis. Therefore, we studied WNT-5A expressions in mouse and human fibrotic livers and examined the relation between WNT-5A and various fibrosis-associated growth factors, cytokines, and extracellular matrix proteins. WNT-5A gene and protein expressions were significantly increased in fibrotic mouse and human livers compared with healthy livers. Regression or therapeutic intervention in mice resulted in decreased hepatic WNT-5A levels paralleled by lower collagen levels. Immunohistochemical analysis showed WNT-5A staining in fibrotic septa colocalizing with desmin staining indicating WNT-5A expression in myofibroblasts. In vitro studies confirmed WNT-5A expression in this cell type and showed that TGF-β significantly enhanced WNT-5A expression in contrast to PDGF-BB and proinflammatory cytokines IL-1β and TNF-α. Additionally, TGF-β induces the expression of the WNT receptors FZD2 and FZD8. After silencing of WNT-5A, reduced levels of collagen type I, vimentin, and fibronectin in TGF-β-stimulated myofibroblasts were measured compared with nonsilencing siRNA-treated controls. Interestingly, the antifibrotic cytokine IFNγ suppressed WNT-5A in vitro and in vivo. IFNγ-treated fibrotic mice showed significantly less WNT-5A expression compared with untreated fibrotic mice. In conclusion, WNT-5A paralleled collagen I levels in fibrotic mouse and human livers. WNT-5A expression in myofibroblasts is induced by the profibrotic factor TGF-β and plays an important role in TGF-β-induced regulation of fibrotic matrix proteins, whereas its expression can be reversed upon treatment, both in vitro and in vivo.NEW & NOTEWORTHY This study describes the localization and functional role of WNT-5A in human and mouse fibrotic livers. Hepatic WNT-5A expression parallels collagen type I expression. In vivo and in vitro, the myofibroblasts were identified as the key hepatic cells producing WNT-5A. WNT-5A is under control of TGF-β and its activities are primarily profibrotic.

Beljaars|Leonie|L| Daliri|Sara|S| Dijkhuizen|Christa|C| Poelstra|Klaas|K| Gosens|Reinoud|R|

fibrosis
WNT
interferon γ
remodeling
transforming growth factor-β


Lorenz system in the thermodynamic modelling of leukaemia malignancy.

Abstract: The core idea of the proposed thermodynamic modelling of malignancy in leukaemia is entropy arising within normal haematopoiesis. Mathematically its description is supposed to be similar to the Lorenz system of ordinary differential equations for simplified processes of heat flow in fluids. The hypothetical model provides a description of remission and relapse in leukaemia as two hierarchical and qualitatively different states of normal haematopoiesis with their own phase spaces. Phase space transition is possible through pitchfork bifurcation, which is considered the common symmetrical scenario for relapse, induced remission and the spontaneous remission of leukaemia. Cytopenia is regarded as an adaptive reaction of haematopoiesis to an increase in entropy caused by leukaemia clones. The following predictions are formulated: a) the percentage of leukaemia cells in marrow as a criterion of remission or relapse is not necessarily constant but is a variable value; b) the probability of remission depends upon normal haematopoiesis reaching bifurcation; c) the duration of remission depends upon the eradication of leukaemia cells through induction or consolidation therapies; d) excessively high doses of chemotherapy in consolidation may induce relapse.

Lorenz system
Thermodynamics
Leukaemia
Entropy
Relapse
Spontaneous remission


What the Future Holds and When: A Description-Experience Gap in Intertemporal Choice.

Abstract: Uncertainty about the waiting time before obtaining an outcome is integral to intertemporal choice. Here, we showed that people express different time preferences depending on how they learn about this temporal uncertainty. In two studies, people chose between pairs of options: one with a single, sure delay and the other involving multiple, probabilistic delays (a lottery). The probability of each delay occurring either was explicitly described (timing risk) or could be learned through experiential sampling (timing uncertainty; the delay itself was not experienced). When the shorter delay was rare, people preferred the lottery more often when it was described than when it was experienced. When the longer delay was rare, this pattern was reversed. Modeling analyses suggested that underexperiencing rare delays and different patterns of probability weighting contribute to this description-experience gap. Our results challenge traditional models of intertemporal choice with temporal uncertainty as well as the generality of inverse-S-shaped probability weighting in such choice.

Dai|Junyi|J|0000-0001-7052-2865
Pachur|Thorsten|T|
Pleskac|Timothy J|TJ|
Hertwig|Ralph|R|

intertemporal choice
temporal uncertainty
description–experience gap
hierarchical Bayesian modeling
open data
open materials
preregistered
rank-dependent discounted-utility model


Small bowel perforation caused by applicator implantation in high-dose-rate interstitial brachytherapy for recurrent pelvic tumor: a case report.

Abstract: High-dose-rate interstitial brachytherapy (HDR-ISBT) is widely used for the treatment of pelvic tumors. However, there are a few reports on complications of applicator implantation in HDR-ISBT. We describe a case of small bowel perforation caused by applicator implantation in an 82-year-old male patient with recurrence tumor in the pelvis treated with HDR-ISBT. Eventually, the patient underwent laparotomy and partial intestinal resection. We recognized the site where the applicator was inserted into a part of the tumor on the mucosal surface. Pathological examination confirmed that the tumor had infiltrated the small intestine directly and that the infiltrated part had reached the submucosa. This is the first published report about small bowel perforation caused by applicator implantation. In cases where intestinal infiltration of the tumor is suspected, HDR-ISBT should be performed with maximum caution.

Masui|Koji|K| Yamazaki|Hideya|H| Suzuki|Gen|G| Shimizu|Daisuke|D| Takenaka|Tadashi|T| Yamada|Yasuhiro|Y| Okihara|Koji|K| Kuriu|Yoshiaki|Y| Yamada|Kei|K|


Natural History and Growth Patterns of Incidentally Discovered Diffusely Infiltrating Low-Grade Gliomas: A Volumetric Study.

Abstract:
BACKGROUND
Incidentally discovered diffusely infiltrating low-grade gliomas (iDLGGs) are rare findings on neuroimaging that pose a challenge to neurosurgeons. There is a paucity of data regarding the natural history of these lesions, and thus management is controversial. We characterize the growth rates and patterns of iDLGGs in a cohort of patients who underwent serial magnetic resonance imaging before surgical treatment.

METHODS
We performed a retrospective review of all adult patients (≥18 years old) with diffuse low-grade glioma diagnosed at our institution between April 2004 and April 2016. iDLGG was defined as any lesion discovered on computed tomography or magnetic resonance imaging performed for reasons and/or symptoms not attributable to the lesion and confirmed on histopathology as low-grade glioma. Tumor growth rates and patterns of growth were analyzed in patients who had serial imaging available.

RESULTS
Inclusion criteria were met by 15 patients. Mean velocity of diametric expansion was 2.93 mm/year. Of 15 patients, 11 (73.3%) had tumors with an exponential growth pattern, and 4 (26.7%) had a linear growth pattern. Initial tumor volume was positively correlated (r = 0.78) with velocity of diametric expansion.

CONCLUSIONS
iDLGGs grow over time, and most exhibit an exponential pattern of growth. Tumor volume at the time of diagnosis is predictive of a faster growth rate, but not the pattern of growth.

Opoku-Darko|Michael|M|
Eagles|Matthew E|ME|
Cadieux|Magalie|M|
Isaacs|Albert M|AM|
Kelly|John J P|JJP|

D000328:Adult
D000367:Age Factors
D001932:Brain Neoplasms
D015331:Cohort Studies
D018450:Disease Progression
D005260:Female
D005910:Glioma
D006801:Humans
D033162:Incidental Findings
D008279:Magnetic Resonance Imaging
D008297:Male
D008875:Middle Aged
D060787:Neoplasm Grading
D059906:Neuroimaging
D012189:Retrospective Studies
D012307:Risk Factors
D014057:Tomography, X-Ray Computed
D055815:Young Adult

Natural history
Incidental
Low-grade glioma
Growth rate
Asymptomatic


Effect of the Affordable Care Act on breast cancer presentation at a safety net hospital.

Abstract:
INTRODUCTION
The Affordable Care Act (ACA) mandated the expansion of Medicaid in order to increase access to health care services. We examined the effect of the ACA on breast cancer screening and diagnosis at a Los Angeles safety net hospital.

METHODS
We performed a retrospective review of breast cancer patients treated at our institution. We compared two cohorts: patients diagnosed with breast cancer in the years 2011-2012 (pre-ACA) vs. 2015-2016 (post-ACA).

RESULTS
There were no differences in number of screening mammograms performed, age at diagnosis, mammography-detected cancers, or clinical stage at diagnosis. There was a significant decrease in the number of patients who reported as self-pay (34% vs. 6%, p < 0.0001).

CONCLUSION
In the 2-year period following ACA implementation, there was limited impact on breast cancer presentation at a safety-net hospital. Long-term follow-up across different healthcare systems is necessary to fully evaluate the global impact of the ACA on breast cancer care.

Satyananda|Vikas|V|
Ozao-Choy|Junko|J|
Dauphine|Christine|C|
Chen|Kathryn T|KT|

D058991:Patient Protection and Affordable Care Act

Affordable Care Act
Breast cancer
Safety-net hospital
Health disparities


Cutaneous Photoprotection: A Review of the Current Status and Evolving Strategies.

Abstract: Ultraviolet radiation (UVR) exposure is well established as the major environmental risk factor for the development of melanoma, cutaneous squamous cell carcinoma (cSCC), and basal cell carcinoma (BCC). Additional risk factors including genetic mutations, other environmental agents, and immune status are important in modulating the effects of UVR. Dermatologists advocate a multi-pronged approach to minimizing UVR exposure including lifestyle modifications, UVR protective clothing, and topically applied sun-protective products, i.e. sunscreen. New Federal Drug Administration (FDA) regulations on sunscreen have brought certain long-standing ingredients in sunscreen products under scrutiny. The FDA's proposed rule for over the counter (OTC) monograph states that the inorganic sunscreens, zinc oxide and titanium dioxide, were found to be "generally recognized as safe and effective," but cite insufficient evidence to grant organic sunscreens the same designation. This proposed rule by the FDA and our increasing understanding of multifactorial mechanisms of UVR damage are an impetus for innovation and advances in sun protective technology. A complete set of strategies designed to limit the risk of UV-induced skin cell malignant transformation and tumor development must address the fuller consideration of genetic, environmental, and immune factors that cooperatively drive cutaneous carcinogenesis. Recent advances in our understanding of the biochemical processes underpinning UVR associated cutaneous cellular damage, genotoxicity, and clonal expansion provide investigators with a spectrum of opportunities for technologic innovation in the prevention of skin cancer. Strategies to improve upon current topical sunscreen formulations have strived for broader UVR spectral coverage, more favorable aesthetics, increased adherence, and minimal penetration into the living epidermis. In addition to improved sunscreens, future topical therapies may target processes within the epidermis that contribute to carcinogenesis. These include reactive species quenching, delivery of DNA repair enzymes, and targeting of cytokines essential to the proliferation of mutant keratinocytes.

D004781:Environmental Exposure
D006801:Humans
D018462:Photobiology
D012878:Skin Neoplasms
D059405:Sun Protection Factor
D013473:Sunscreening Agents
D014466:Ultraviolet Rays

ultraviolet radiation
Sunscreen
skin cancer
photobiology


Performance of at-home self-collected saliva and nasal-oropharyngeal swabs in the surveillance of COVID-19.

Abstract: Background: SARS-CoV-2 quickly spreads in the worldwide population, imposing social restrictions to control the infection, being the massive testing another essential strategy to break the chain of transmission. Aim: To compare the performance of at-home self-collected samples - saliva and combined nasal-oropharyngeal swabs (NOP) - for SARS-CoV-2 detection in a telemedicine platform for COVID-19 surveillance. Material and methods: We analyzed 201 patients who met the criteria of suspected COVID-19. NOP sampling was combined (nostrils and oropharynx) and saliva collected using a cotton pad device. Detection of SARS-COV-2 was performed by using the Altona RealStar® SARS-CoV-2 RT-PCR Kit 1.0.  Results: There was an overall significant agreement (κ coefficient value of 0.58) between saliva and NOP. Considering results in either sample, 70 patients positive for SARS-CoV-2 were identified, with 52/70 being positive in NOP and 55/70 in saliva. This corresponds to sensitivities of 74.2% (95% CI; 63.7% to 83.1%) for NOP and 78.6% (95% CI; 67.6% to 86.6%) for saliva. Conclusion: Our data show the feasibility of using at-home self-collected samples (especially saliva), as an adequate alternative for SARS-CoV-2 detection. This new approach of testing can be useful to develop strategies for COVID-19 surveillance and for guiding public health decisions.

Saliva
telemedicine
PCR
coronavirus
infection control
primary health care


Photocatalytic Activity of Cellulose Acetate Nanoceria/Pt Hybrid Mats Driven by Visible Light Irradiation.

Abstract: A photocatalytic system for the degradation of aqueous organic pollutants under visible light irradiation is obtained by an innovative approach based on ceria/platinum (Pt) hybrid nanoclusters on cellulose acetate fibrous membranes. The catalytic materials are fabricated by supersonic beam deposition of Pt nanoclusters directly on the surface of electrospun cellulose acetate fibrous mats, pre-loaded with a cerium salt precursor that is transformed into ceria nanoparticles directly in the solid mats by a simple thermal treatment. The presence of Pt enhances the oxygen vacancies on the surface of the formed ceria nanoparticles and reduces their band gap, resulting in a significant improvement of the photocatalytic performance of the composite mats under visible light irradiation. Upon the appropriate pretreatment and visible light irradiation, we prove that the most efficient mats, with both ceria nanoparticles and Pt nanoclusters, present a degradation efficiency of methylene blue of 70% and a photodegradation rate improved by about five times compared to the ceria loaded samples, without Pt. The present results bring a significant improvement of the photocatalytic performance of polymeric nanocomposite fibrous systems under visible light irradiation, for efficient wastewater treatment applications.

ceria nanoparticles
photodegradation
ROS detection
fiber mats
platinum nanoclusters


Deletion of indoleamine 2,3 dioxygenase (Ido)1 but not Ido2 exacerbates disease symptoms of MOG35-55-induced experimental autoimmune encephalomyelitis.

Abstract: Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS) with pathological features of inflammation, demyelination, and neurodegeneration. Several lines of evidence suggest that the enzymes indoleamine 2,3-dioxygenase (Ido)1 and/or Ido2 influences susceptibility to autoimmune diseases. Deletion of Ido1 exacerbates experimental autoimmune encephalomyelitis (EAE) an animal model of MS. However, no data exist on the role of Ido2 in the pathogenesis of EAE. We investigated whether deletion of Ido2 affected the pathogenesis of EAE. Temporal expression of interferon gamma (Ifng), Ido1 variants, Ido2 variants, as well as genes encoding enzymes of the kynurenine pathway in the spleen and spinal cord of C57BL/6 mice with or without EAE were determined by RT-qPCR. Moreover, EAE was induced in C57BL/6, two Ido1 knockout strains (Ido1KO and Ido1TK) and one Ido2 knockout mouse strain (Ido2-/-) and disease monitored by clinical scores and weight change. Performance on the rotarod was performed on days 0, 5, 10 and 15 post induction. The extent of demyelination in the spinal cord was determined after staining with Oil red O. The development of EAE altered gene expression in both the spleen and spinal cord. Deletion of Ido1 exacerbated the clinical symptoms of EAE. In stark contrast, EAE in Ido2-/- mice did not differ clinically or histologically from control mice. These results confirm a protective role for Ido1, on the pathogenesis of MOG35-55-induced EAE in C57BL/6J mice.

Wetzel|Lisa A|LA|
Hurtado|Myrna|M|
MacDowell Kaswan|Zoe A|ZA|
McCusker|Robert H|RH|
Steelman|Andrew J|AJ|


Ice age fish in a warming world: minimal variation in thermal acclimation capacity among lake trout (Salvelinus namaycush) populations.

Abstract: In the face of climate change, the persistence of cold-adapted species will depend on their adaptive capacity for physiological traits within and among populations. The lake trout (Salvelinus namaycush) is a cold-adapted salmonid and a relict from the last ice age that is well suited as a model species for studying the predicted effects of climate change on coldwater fishes. We investigated the thermal acclimation capacity of upper temperature resistance and metabolism of lake trout from four populations across four acclimation temperatures. Individuals were reared from egg fertilization onward in a common environment and, at 2 years of age, were acclimated to 8, 11, 15 or 19°C. Although one population had a slightly higher maximal metabolic rate (MMR), higher metabolic scope for activity and faster metabolic recovery across all temperatures, there was no interpopulation variation for critical thermal maximum (CTM) or routine metabolic rate (RMR) or for the thermal acclimation capacity of CTM, RMR, MMR or metabolic scope. Across the four acclimation temperatures, there was a 3°C maximal increase in CTM and 3-fold increase in RMR for all populations. Above 15°C, a decline in MMR and increase in RMR resulted in sharply reduced metabolic scope for all populations acclimated at 19°C. Together, these data suggest there is limited variation among lake trout populations in thermal physiology or capacity for thermal acclimatization, and that climate change may impact lake trout populations in a similar manner across a wide geographical range. Understanding the effect of elevated temperatures on the thermal physiology of this economically and ecologically important cold-adapted species will help inform management and conservation strategies for the long-term sustainability of lake trout populations.

Kelly|Nicholas I|NI|
Burness|Gary|G|
McDermid|Jenni L|JL|
Wilson|Chris C|CC|

thermal acclimation
Salvelinus namaycush
Climate change
physiology
temperature
metabolic rate


Domestic Violence Between Childhood Incest and Re-victimization: A Study Among Anti-violence Centers in Italy.

Abstract: The study focuses on a specific problem in the area of child sexual abuse (CSA), which is still under-researched: the relationship between incest and adult female re-victimization treatment within the ambit of domestic violence in Italian centers. About 112 anti-violence centers were contacted, but only 13 participated and only 16 psychologists were interviewed to reconstruct the biographies of 32 victims. The study aimed to examine if and how the service centers recognized and dealt with the problem of re-victimization among survivors based on psychologists' narrations. Findings showed that the description of perpetrators revealed not only sexual abuse was perpetrated, but also psychological and physical abuse. About half of mothers did not come to their daughters' aid and those who cooperated with the abusers had mostly suffered from CSA at a time in their life. Only three mothers did help their daughters in contacting the anti-violence centers. However, most of the service centers were not concerned with the relationship between incest and domestic re-victimization, while those who considered the problem, dealt with it only on the practitioner-patient level. In addition, despite the psychologists used professional and empathetic language, they disclosed their high emotional involvement and a genuine bewilderment. A discussion on the need to standardize the psychotherapeutic support given to these re-victimized women was presented, with a critique to the un-discriminated de-pathologization approach adopted by almost all anti-violence centers. In particular, we wanted to underline the fact that, although, this approach is useful in treating victims who were not abused during infancy, it could be insufficient for women who suffered from incest.

domestic violence
childhood incest
de-pathologization
children sexual abuse (CSA)
re-victimization gender-based violence


Attitudes and availability: A comparison of naloxone dispensing across chain and independent pharmacies in rural and urban areas in Alabama.

Abstract:
BACKGROUND
Fatal opioid overdoses remain the leading cause of accidental deaths in the United States, which have contributed to implementation of standing order laws that allow pharmacists to dispense naloxone to patients. Although pharmacy distribution of naloxone is a promising approach to increase access to this intervention, understanding barriers preventing greater uptake of this service is needed.

METHODS
Data for the current study were collected via telephone survey assessing the availability of various formulations of naloxone at chain and independent pharmacies in rural and urban areas in Birmingham, Alabama (N = 222). Pharmacists' attitudes toward naloxone and potential barriers of pharmacy naloxone distribution were also assessed. One-way analysis of variance (ANOVA) and logistic regression analyses were utilized to examine differences in stocking of naloxone in chain and independent pharmacies and to determine predictors of the number of kits dispensed by pharmacies.

RESULTS
Independent pharmacies were less likely to have naloxone in stock, especially those in rural areas. Furthermore, rural pharmacies required more time to obtain all four formulations of naloxone, and offered less extensive training on naloxone use. Pharmacists endorsing the belief that naloxone allows avoidance of emergent treatment in an overdose situation was associated with fewer dispensed kits by the pharmacies. Over 80% of pharmacists endorsed at least one negative belief about naloxone (e.g., allowing riskier opioid use). Pharmacists noted cost to patients and the pharmacy as contributing to not dispensing more naloxone kits.

CONCLUSION
The current study demonstrates the lower availability of naloxone stocked at pharmacies in independent versus chain pharmacies, particularly in rural communities. This study also highlights several barriers preventing greater naloxone dispensing including pharmacists' attitudes and costs of naloxone. The potential benefit of standing order laws is not being fully actualized due to the structural and attitudinal barriers identified in this study. Strategies to increase naloxone access through pharmacy dispensing are discussed.

Sisson|Michelle L|ML|
McMahan|Kristina B|KB|
Chichester|Keith R|KR|
Galbraith|James W|JW|
Cropsey|Karen L|KL|


Human Umbilical Cord Mesenchymal Stem Cells Preserve Adult Newborn Neurons and Reduce Neurological Injury after Cerebral Ischemia by Reducing the Number of Hypertrophic Microglia/Macrophages.

Abstract: Microglia are the first source of a neuroinflammatory cascade, which seems to be involved in every phase of stroke-related neuronal damage. Two weeks after transient middle cerebral artery occlusion (MCAO), vehicle-treated rats displayed higher numbers of total ionized calcium-binding adaptor molecule 1 (Iba-1)-positive cells, greater cell body areas of Iba-1-positive cells, and higher numbers of hypertrophic Iba-1-positive cells (with a cell body area over 80 μm2) in the ipsilateral ischemic brain regions including the frontal cortex, striatum, and parietal cortex. In addition, MCAO decreased the number of migrating neuroblasts (or DCX- and 5-ethynyl-2'-deoxyuridine-positive cells) in the cortex, subventricular zone, and hippocampus of the ischemic brain, followed by neurological injury (including brain infarct and neurological deficits). Intravenous administration of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs; 1 × 106 or 4 × 106) at 24 h after MCAO reduced neurological injury, decreased the number of hypertrophic microglia/macrophages, and increased the number of newborn neurons in rat brains. Thus, the accumulation of hypertrophic microglia/macrophages seems to be detrimental to neurogenesis after stroke. Treatment with hUC-MSCs preserved adult newborn neurons and reduced functional impairment after transient cerebral ischemia by reducing the number of hypertrophic microglia/macrophages.

Lin|Willie|W|
Hsuan|Yogi Chang-Yo|YC|
Lin|Mao-Tsun|MT|
Kuo|Ting-Wei|TW|
Lin|Cheng-Hsien|CH|
Su|Yu-Chin|YC|
Niu|Ko-Chi|KC|
Chang|Ching-Ping|CP|0000-0003-0890-9414
Lin|Hung-Jung|HJ|

umbilical cord mesenchymal stem cells
microglia
stroke
cell therapy


Guideline No. 422a: Menopause: Vasomotor Symptoms, Prescription Therapeutic Agents, Complementary and Alternative Medicine, Nutrition, and Lifestyle.

Abstract:
OBJECTIVE
Provide strategies for improving the care of perimenopausal and postmenopausal women based on the most recent published evidence.

TARGET POPULATION
Perimenopausal and postmenopausal women.

BENEFITS, HARMS, AND COSTS
Target population will benefit from the most recent published scientific evidence provided via the information from their health care provider. No harms or costs are involved with this information since women will have the opportunity to choose among the different therapeutic options for the management of the symptoms and morbidities associated with menopause, including the option to choose no treatment.

EVIDENCE
Databases consulted were PubMed, MEDLINE, and the Cochrane Library for the years 2002-2020, and MeSH search terms were specific for each topic developed through the 7 chapters.

VALIDATION METHODS
The authors rated the quality of evidence and strength of recommendations using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach. See online Appendix A (Tables A1 for definitions and A2 for interpretations of strong and weak recommendations). INTENDED AUDIENCE: physicians, including gynaecologists, obstetricians, family physicians, internists, emergency medicine specialists; nurses, including registered nurses and nurse practitioners; pharmacists; medical trainees, including medical students, residents, fellows; and other providers of health care for the target population.

SUMMARY STATEMENTS
RECOMMENDATIONS.

Yuksel|Nese|N| Evaniuk|Debra|D| Huang|Lina|L| Malhotra|Unjali|U| Blake|Jennifer|J| Wolfman|Wendy|W| Fortier|Michel|M|

menopause
vasomotor symptoms
management
complementary therapy
drug therapy
hormone therapy


Assessment of Clinical Outcome of Lumbar Transforaminal Foraminoplasty in Patients with Lumbar Spinal Stenosis.

Abstract:
BACKGROUND
Lumbar spinal stenosis (LSS), a common spinal disorder that negatively affects quality of life, is a disabling condition accompanied by back pain, leg pain, and claudication. Lumbar foraminal stenosis (LFS) is often accompanied by lumbar central stenosis (LCS) and conservative treatment is often ineffective. A surgical approach, including a minimally invasive technique, is usually recommended for the conservative treatment of refractory conditions. To achieve effective decompression of LSS, a specially designed new instrument for lumbar transforaminal foraminoplasty (TFFP) can be considered before opting for surgical treatment.

OBJECTIVE
To evaluate the clinical outcomes and safety of TFFP with a specially designed instrument.

STUDY DESIGN
Retrospective design.

SETTING
This research was conducted in a hospital outpatient surgical center.

METHODS
The medical records of 112 patients who underwent TFFP from December 1, 2018 to January 1, 2020, were reviewed. Outcome measures were obtained using the numeric rating scale for pain (NRS pain), Oswestry Disability Index (ODI), and walking distance without pain for functional ability at preprocedure and 1, 3, and 6 months postprocedure. The clinical data and radiologic findings were analyzed to evaluate correlations between predictive factors and efficacy of TFFP.

RESULTS
Among 112 patients who underwent TFFP, 110 were accessed and analyzed. The percentage of successful responders was 59.1%, 73.6%, and 74.5 % of 110 patients at one, 3, and 6 months, respectively. The NRS pain score, ODI, and duration of walking without radicular pain were improved significantly at the one-, 3-, and 6-month follow-up periods (all P < 0.001). No serious adverse events occurred during this study.

LIMITATIONS
The limitations of this study include the possibility of bias due to nonrandomized patient selection.

CONCLUSION
TFFP using the Foramoon® device (Mcarekorea, Seongnam-si, Gyeonggi-do, Republic of Korea) appeared to be effective for managing patients with LFS and LCS, who were refractory to conservative care.

Lee|Sung Hyun|SH|
Hwang|Sung-Ha|SH|
Moon|Young-Kwan|YK|
Bae|Hyun Min|HM|
Moon|Dong Eon|DE|

transforaminal foraminoplasty
Lumbar spinal stenosis
lumbar foraminal stenosis
lumbar central stenosis
radicular pain
ligament flavum
neural compression
transverse ligament


How Well Is CHIP Addressing Health Care Access and Affordability for Children?

Abstract:
OBJECTIVE
We examine how access to care and care experiences under the Children's Health Insurance Program (CHIP) compared to private coverage and being uninsured in 10 states.

METHODS
We report on findings from a 2012 survey of CHIP enrollees in 10 states. We examined a range of health care access and use measures among CHIP enrollees. Comparisons of the experiences of established CHIP enrollees to the experiences of uninsured and privately insured children were used to estimate differences in children's health care.

RESULTS
Children with CHIP coverage had substantially better access to care across a range of outcomes, other things being equal, particularly compared to those with no coverage. Compared to being uninsured, CHIP enrollees were more likely to have specialty and mental health visits and to receive prescription drugs; and their parents were much more likely to feel confident in meeting the child's health care needs and were less likely to have trouble finding providers. CHIP enrollees were less likely to have unmet needs, but 1 in 4 had at least 1 unmet need. Compared to being privately insured, CHIP enrollees had generally similar health care use and unmet needs. Additionally, CHIP enrollees had lower financial burden related to their health care needs. The findings were generally robust with respect to alternative specifications and subgroup analyses, and they corroborated findings of previous studies.

CONCLUSIONS
Enrolling more of the uninsured children who are eligible for CHIP improved their access to a range of care, including specialty and mental health services, and reduced the financial burden of meeting their health care needs; however, we found room for improvement in CHIP enrollees' access to care.

Clemans-Cope|Lisa|L| Kenney|Genevieve|G| Waidmann|Timothy|T| Huntress|Michael|M| Anderson|Nathaniel|N|

D065326:Children's Health Insurance Program

comparison of health insurance coverage types