Seed biopriming with plant growth promoting rhizobacteria: a review.

Abstract: Beneficial microbes are applied to the soil and plant tissues directly or through seed inoculation, whereas soil application is preferred when there is risk of inhibitors or antagonistic microbes on the plant tissues. Insufficient survival of the microorganisms, hindrance in application of fungicides to the seeds and exposure to heat and sunlight in subsequent seed storage in conventional inoculation methods force to explore appropriate and efficient bacterial application method. Seed priming, where seeds are hydrated to activate metabolism without actual germination followed by drying, increases the germination, stand establishment and stress tolerance in different crops. Seed priming with living bacterial inoculum is termed as biopriming that involves the application of plant growth promoting rhizobacteria. It increases speed and uniformity of germination; also ensures rapid, uniform and high establishment of crops; and hence improves harvest quality and yield. Seed biopriming allows the bacteria to enter/adhere the seeds and also acclimatization of bacteria in the prevalent conditions. This review focuses on methods used for biopriming, and also the role in improving crop productivity and stress tolerance along with prospects of this technology. The comparison of methods being followed is also reviewed proposing biopriming as a promising technique for application of beneficial microbes to the seeds.

Mahmood|Ahmad|A|
Turgay|Oğuz Can|OC|
Farooq|Muhammad|M|
Hayat|Rifat|R|

biopriming
plant growth promoting rhizobacteria
inoculation


The role of white matter abnormality in the left anterior corona radiata: In relation to formal thought disorder in patients with schizophrenia.

Abstract: White matter abnormality has been widely reported in patients with schizophrenia (Sz). However, few studies have focused on the relationship between the white matter deficit and formal thought disorder (FTD). Moreover, the role of genetic high risk in FTD-related white matter deficit remains unclear. The present study recruited 46 Sz patients, 18 unaffected first-degree relatives of Sz patients, and 29 healthy controls. There was a widespread fractional anisotropy (FA) reduction in Sz. In addition, reduced FA in the left anterior corona radiata was related to more severe FTD symptoms in Sz. However, the genetic high-risk group only showed lower mean FA in the left anterior limb of the internal capsule than healthy controls. Our findings suggest that abnormality in the left anterior corona radiata may only occur in Sz but not in the genetic high-risk group. Such an abnormality might be associated with the severity of FTD symptoms. Meanwhile, genetic vulnerability may contribute to the abnormality in the left anterior limb of the internal capsule. Better analytical methods are needed to validate our results.

Chen|Shan|S| Tang|Yingying|Y| Fan|Xiaoduo|X| Qiao|Yi|Y| Wang|Jijun|J| Wen|Hun|H| Wang|Wenzheng|W| Wang|Hongyan|H| Yang|Fuzhong|F| Sheng|Jianhua|J|

Formal thought disorder
Schizophrenia
Genetic high risk
Diffusion tensor imaging


Differential pH Dynamics in Synaptic Vesicles From Intact Glutamatergic and GABAergic Synapses.

Abstract: Synaptic transmission requires the presynaptic release of neurotransmitter from synaptic vesicles (SVs) onto the postsynaptic neuron. Vesicular neurotransmitter transporter proteins, which use a V-ATPase-generated proton gradient, play a crucial role in packaging neurotransmitter into SVs. Recent work has revealed different proton dynamics in SVs expressing the vesicular glutamate transporter (VGLUT) or the vesicular GABA transporter (VGAT) proteins. At the whole synapse level, this results in different steady-state pH and different reacidification dynamics during SV recycling (Egashira et al., 2016). In isolated SVs, the presence of VGAT causes a higher steady state pH, which is correlated with a faster proton efflux rate (Farsi et al., 2016). To address whether proton efflux from GABAergic and glutamatergic SVs in intact synapses differs, we applied a glutamatergic- or GABAergic neuron-specific expression strategy (Chang et al., 2014) to express a genetically encoded pH sensor (synaptophysin pHluorin; SypHy) and/or light-activated proton pump (pHoenix; (Rost et al., 2015). We confirm, with SypHy post-stimulation fluorescence dynamics, that the pH profile of recycling GABAergic SVs differs from that of recycling glutamatergic SVs (Egashira et al., 2016). Using light-activation of pHoenix in pH-neutral vesicles, we investigated the pH dynamics of actively filling vesicles, and could show that proton efflux from GABAergic SVs is indeed initially faster than glutamatergic SVs in intact synapses. Finally, we compared the filling rate of empty glutamatergic and GABAergic vesicles using pHoenix as a proton source, and find a slightly faster filling of glutamatergic vs. GABAergic SVs.

Herman|Melissa A|MA|
Trimbuch|Thorsten|T|
Rosenmund|Christian|C|

synaptic vesicle
proton gradient
VGLUT
VGAT
vesicular transporter


A study of the effect of nasal modes of ventilation on the incidence of gastro-oesophageal reflux in preterm neonates.

Abstract:
BACKGROUND
Nasal modes of respiratory support cause variable amounts of gastric dilatation which may increase gastro-oesophageal reflux (GER) in preterms. To compare the incidence of GER in nasally ventilated, preterm babies with controls (babies not on ventilation).

TYPE OF STUDY
A prospective, observational comparative study.

METHOD
Twenty-three preterm babies of gestational age 28-36 weeks and weight ranging between 1,000 g and < 2,500 g on either nasal continuous positive airway pressure (nCPAP) or nasal intermittent positive pressure venti-lation (nIPPV) were assessed for GER. They were compared with controls not on ventilation some of who were test babies when off ventilation (subgroup A) and some were unrelated babies not on ventilator but matched for gestational age and weight with test babies (subgroup B). All babies were subjected to continuous, oesophageal pH monitoring with dual sensor (upper and lower oesophageal) catheters. Reflux index (RI) was calculated as the percentage of study time the lower oesophageal pH was < 4. Primary outcome was the RI in the test and controls groups. Secondary outcome was the temporal relation of the reflux with symptoms if any. Numerical data were shown as mean with standard deviation and statistical comparisons were done using the χ(2)-test, Fischer test, and t-test wherever applicable.

RESULTS
The RI was higher in ventilated babies as compared to the control group, particularly in the subgroup A, where test babies formed their own controls. Grade IV reflux (7 cases) was seen only in the ventilated babies. There was no difference in the incidence of GER in babies on nCPAP as compared with nIPPV. Grade IV reflux could not be reliably predicted by RI alone. No definite temporal relation between episodes of reflux and symptoms could be determined in this study.

CONCLUSION
There is an increase in GER in preterms on nasal modes of ventilation. A combination of upper (pharyngeal) and lower oesophageal sensors are preferred to a single lower oesophageal sensor when assessing GER by oesophageal pHmetry in neonates.

gastro-oesophageal reflux
preterm
nasal ventilation
CPAP


Heat and Humidity in the City: Neighborhood Heat Index Variability in a Mid-Sized City in the Southeastern United States.

Abstract: Daily weather conditions for an entire city are usually represented by a single weather station, often located at a nearby airport. This resolution of atmospheric data fails to recognize the microscale climatic variability associated with land use decisions across and within urban neighborhoods. This study uses heat index, a measure of the combined effects of temperature and humidity, to assess the variability of heat exposure from ten weather stations across four urban neighborhoods and two control locations (downtown and in a nearby nature center) in Knoxville, Tennessee, USA. Results suggest that trees may negate a portion of excess urban heat, but are also associated with greater humidity. As a result, the heat index of locations with more trees is significantly higher than downtown and areas with fewer trees. Trees may also reduce heat stress by shading individuals from incoming radiation, though this is not considered in this study. Greater amounts of impervious surfaces correspond with reduced evapotranspiration and greater runoff, in terms of overall mass balance, leading to a higher temperature, but lower relative humidity. Heat index and relative humidity were found to significantly vary between locations with different tree cover and neighborhood characteristics for the full study time period as well as for the top 10% of heat index days. This work demonstrates the need for high-resolution climate data and the use of additional measures beyond temperature to understand urban neighborhood exposure to extreme heat, and expresses the importance of considering vulnerability differences among residents when analyzing neighborhood-scale impacts.

Hass|Alisa L|AL|
Ellis|Kelsey N|KN|
Reyes Mason|Lisa|L|
Hathaway|Jon M|JM|
Howe|David A|DA|

heat exposure
impervious surface
canopy
microclimate
urban heat island


Are we getting necrotizing soft tissue infections right? A 10-year review.

Abstract:
BACKGROUND
The Alfred Hospital is a referral centre for necrotizing soft tissue infections (NSTIs) in the state of Victoria and receives around 20 such patients each year. We sought to compare our practice and outcomes against published data, and to examine management at referring hospitals to determine whether adjustments to current practices are required.

METHODS
A retrospective chart review of patients admitted to the Alfred Hospital between 1 January 2001 and 31 December 2010 with a diagnosis of necrotizing fasciitis was conducted. Demographic, etiologic, treatment and outcome data were collected and analysed.

RESULTS
Two hundred and nineteen patients were identified with a mean age of 54.76 years and a preponderance of men (63.47%). The overall mortality rate for the patient group was 15.98%. More than 80% of patients were transferred from another facility. Nearly 40% of patients did not undergo surgical debridement within 24 h of presentation to a hospital and 30.6% were not debrided prior to transfer. Patients underwent a median of three procedures at the Alfred Hospital and the majority of patients required admission to the intensive care unit (68.95%).

CONCLUSION
NSTIs remain a surgical emergency with high rates of mortality and resource requirements. The mortality rate at our institution compares well with other published series. Many patients experienced delays before undergoing debridement and in many cases were transferred without debridement. The trend towards transferring NSTI patients to centres accustomed to treating burns and major trauma seems logical, but should not delay life-saving surgical debridement. Timing of transfer does not seem to affect mortality.

Proud|David|D|
Bruscino Raiola|Frank|F|
Holden|Dane|D|
Paul|Eldho|E|
Capstick|Robert|R|
Khoo|Amy|A|

D000328:Adult
D000368:Aged
D000900:Anti-Bacterial Agents
D015331:Cohort Studies
D003131:Combined Modality Therapy
D016001:Confidence Intervals
D003646:Debridement
D019115:Fasciitis, Necrotizing
D005260:Female
D005500:Follow-Up Studies
D017052:Hospital Mortality
D006785:Hospitals, University
D006801:Humans
D006931:Hyperbaric Oxygenation
D007902:Length of Stay
D016015:Logistic Models
D008297:Male
D008875:Middle Aged
D017063:Outcome Assessment, Health Care
D012017:Referral and Consultation
D012189:Retrospective Studies
D018570:Risk Assessment
D012720:Severity of Illness Index
D015996:Survival Rate
D016896:Treatment Outcome
D014739:Victoria

necrotizing fasciitis
Fournier's gangrene


Transcriptome analysis of the zebrafish atoh7-/- Mutant, lakritz, highlights Atoh7-dependent genetic networks with potential implications for human eye diseases.

Abstract: Expression of the bHLH transcription protein Atoh7 is a crucial factor conferring competence to retinal progenitor cells for the development of retinal ganglion cells. Several studies have emerged establishing ATOH7 as a retinal disease gene. Remarkably, such studies uncovered ATOH7 variants associated with global eye defects including optic nerve hypoplasia, microphthalmia, retinal vascular disorders, and glaucoma. The complex genetic networks and cellular decisions arising downstream of atoh7 expression, and how their dysregulation cause development of such disease traits remains unknown. To begin to understand such Atoh7-dependent events in vivo, we performed transcriptome analysis of wild-type and atoh7 mutant (lakritz) zebrafish embryos at the onset of retinal ganglion cell differentiation. We investigated in silico interplays of atoh7 and other disease-related genes and pathways. By network reconstruction analysis of differentially expressed genes, we identified gene clusters enriched in retinal development, cell cycle, chromatin remodeling, stress response, and Wnt pathways. By weighted gene coexpression network, we identified coexpression modules affected by the mutation and enriched in retina development genes tightly connected to atoh7. We established the groundwork whereby Atoh7-linked cellular and molecular processes can be investigated in the dynamic multi-tissue environment of the developing normal and diseased vertebrate eye.

Covello|Giuseppina|G|
Rossello|Fernando J|FJ|
Filosi|Michele|M|
Gajardo|Felipe|F|
Duchemin|Anne-Laure|AL|
Tremonti|Beatrice F|BF|
Eichenlaub|Michael|M|
Polo|Jose M|JM|
Powell|David|D|
Ngai|John|J|
Allende|Miguel L|ML|
Domenici|Enrico|E|
Ramialison|Mirana|M|
Poggi|Lucia|L|

transcriptome analysis
retinal ganglion cells
Ath5
human retina
inherited eye diseases


Altered mitochondrial calcium handling and cell death by necroptosis: An emerging paradigm.

Abstract: The classical necroptosis signaling is mediated by death receptors (DRs) that work in synergy with traditional caspase inhibitory signals. Currently, potential therapeutic molecules are in various phases of clinical trials for a spectrum of pathological conditions associated with necroptosis. However, a non-classical model of necroptosis has also emerged over the last decade with a relatively unexplored molecular mechanism. Although in vitro studies and preclinical models have shown its close association with mitochondrial dysfunction (mito-dysfunction), contradictory reports have emerged which complicate its definitiveness. Though impaired mitochondrial calcium ([Ca2+]m) handling is established in necrotic cell death, how this interplay regulates necroptosis is yet to be elucidated. Taking these questions into consideration, we have discussed various molecular aspects of necroptosis with the emerging role of mito-dysfunction. Based on the central role of altered [Ca2+]m handling in mito-dysfunction mediated necroptosis, we have provided a comprehensive molecular insight into this emerging paradigm. Potential reasons for the contradictory findings regarding the role of mito-dysfunction in necroptosis in general and mitochondrial-dependent necroptosis in specific are discussed. We also provide insights into the current understanding of how [Ca2+]m can be a critical determinant in deciding the cell fate under certain pathological conditions, while under others it may be dispensable. Lastly, we have highlighted the key molecular targets which have a direct implication for therapeutic intervention in conditions that are associated with impaired [Ca2+]m handling and cell death by necroptosis.

Mitochondrial calcium
Necroptosis
Death receptors
Apoptosis
MCU
MICU1/2


How Native Prosody Affects Pitch Processing during Word Learning in Limburgian and Dutch Toddlers and Adults.

Abstract: In this study, Limburgian and Dutch 2.5- to 4-year-olds and adults took part in a word learning experiment. Following the procedure employed by Quam and Swingley (2010) and Singh et al. (2014), participants learned two novel word-object mappings. After training, word recognition was tested in correct pronunciation (CP) trials and mispronunciation (MP) trials featuring a pitch change. Since Limburgian is considered a restricted tone language, we expected that the pitch change would hinder word recognition in Limburgian, but not in non-tonal Dutch listeners. Contrary to our expectations, both Limburgian and Dutch children appeared to be sensitive to pitch changes in newly learned words, indicated by a significant decrease in target fixation in MP trials compared to CP trials. Limburgian and Dutch adults showed very strong naming effects in both trial types. The results are discussed against the background of the influence of the native prosodic system.

word learning
Limburgian
word recognition
mispronunciations
bidialectalism
lexical tone
preferential looking


Alkaloids isolated from Tabernaemontana bufalina display xanthine oxidase inhibitory activity.

Abstract: Continued interest in bioactive alkaloids led to the isolation of four undescribed alkaloids along with 74 known ones from the aerial parts of Tabernaemontana bufalina Lour. The structures of the yet undescribed alkaloids were elucidated based on NMR, IR, UV, MS and CD spectroscopic data and X-ray crystal diffraction and, according to the plant source, named as taberhaines A-D (1-4). The known compounds comprised of 66 monoterpenoid indole, three carboline and five isoquinoline alkaloids. Among them, the known apparicine inhibited significantly the activity of xanthine oxidase, which plays an important role for gout, with an IC50 value of 0.65 μM, compared to the standard drug allopurinol (IC50 = 0.60 μM).

Shi|Bao-Bao|BB| Chen|Jing|J| Bao|Mei-Fen|MF| Zeng|Ying|Y| Cai|Xiang-Hai|XH|

D000470:Alkaloids
D020134:Catalytic Domain
D004791:Enzyme Inhibitors
D020128:Inhibitory Concentration 50
D008958:Models, Molecular
D030014:Tabernaemontana
D014969:Xanthine Oxidase

Alkaloid
Apparicine
Taberhaines A–D
Tabernaemontana bufalina apocynaceae
Xanthine oxidase inhibitor


CTX-M-15 in combination with aac(6')-Ib-cr is the most prevalent mechanism of resistance both in Escherichia coli and Klebsiella pneumoniae, including K. pneumoniae ST258, in an ICU in Uruguay.

Abstract: The objectives of this study were (i) to determine the extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae (ESBL-EcKp) clones circulating in an intensive care unit (ICU) in Uruguay between August 2010 and July 2011, (ii) to characterise the ESBL and plasmid-mediated quinolone resistance (PMQR) genes of the studied isolates and (iii) to determine the virulotype of the clinical isolates. Clinical and gut-colonising ESBL-EcKp from ICU patients were studied. Bacterial identification and antibiotic susceptibility determination were performed using a VITEK(®)2 system. Detection of ESBL, KPC and PMQR genes was performed by PCR and sequencing. Clonality was assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). In total, 54 ESBL-EcKp isolates (40 K. pneumoniae and 14 E. coli), with or without PMQR genes, were recovered from 30 of 68 inpatients. Forty-seven isolates were CTX-M-15-producers (36 as a single ESBL and 11 together with CTX-M-14). In addition, four isolates produced CTX-M-14, two produced CTX-M-2 and one produced SHV-5. No carbapenemases were detected either in E. coli or K. pneumoniae isolates. Among the ESBL-producing isolates, 42 also harboured PMQR genes: 27 aac(6')-Ib-cr; 14 aac(6')-Ib-cr and qnrB; and a single isolate carrying only qnrB. K. pneumoniae ST258, ST48 and ST16 and E. coli ST10 and ST405 were detected in 46/54 isolates, including 9 clinical isolates. In conclusion, non-KPC-producing K. pneumoniae ST258 harbouring different ESBL and PMQR genes was the main clone disseminated in the ICU. Extensive surveillance measures must be implemented to prevent the emergence of acquired plasmid-encoded blaKPC by ST258 K. pneumoniae.

Bado|Inés|I|
Gutiérrez|Claudia|C|
García-Fulgueiras|Virginia|V|
Cordeiro|Nicolás F|NF|
Araújo Pirez|Lucía|L|
Seija|Verónica|V|
Bazet|Cristina|C|
Rieppi|Gloria|G|
Vignoli|Rafael|R|

D024901:Drug Resistance, Multiple, Bacterial

Klebsiella pneumoniae
ST258
ESBL
Extended-spectrum β-lactamase


Tempo-Spatial Pattern of Stepharine Accumulation in Stephania Glabra Morphogenic Tissues.

Abstract: Alkaloids attract great attention due to their valuable therapeutic properties. Stepharine, an aporphine alkaloid of Stephania glabra plants, exhibits anti-aging, anti-hypertensive, and anti-viral effects. The distribution of aporphine alkaloids in cell cultures, as well as whole plants is unknown, which hampers the development of bioengineering strategies toward enhancing their production. The spatial distribution of stepharine in cell culture models, plantlets, and mature micropropagated plants was investigated at the cellular and organ levels. Stepharine biosynthesis was found to be highly spatially and temporally regulated during plant development. We proposed that self-intoxication is the most likely reason for the failure of the induction of alkaloid biosynthesis in cell cultures. During somatic embryo development, the toxic load of alkaloids inside the cells increased. Only specialized cell sites such as vascular tissues with companion cells (VT cells), laticifers, and parenchymal cells with inclusions (PI cells) can tolerate the accumulation of alkaloids, and thus circumvent this restriction. S. glabra plants have adapted to toxic pressure by forming an additional transport secretory (laticifer) system and depository PI cells. Postembryonic growth restricts specialized cell site formation during organ development. Future bioengineering strategies should include cultures enriched in the specific cells identified in this study.

D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

stepharine
MALDI-MS
Menispermaceae
alkaloids localization
biotechnology
plant cell culture


Survival of Patients With Angina Pectoris Undergoing Percutaneous Coronary Intervention With Intracoronary Pressure Wire Guidance.

Abstract:
BACKGROUND
Intracoronary pressure wire measurement of fractional flow reserve (FFR) provides decision-making guidance during percutaneous coronary intervention (PCI). However, limited data exist on the effect of FFR on long-term clinical outcomes in patients with stable angina pectoris.

OBJECTIVES
The purpose of this study was to determine the association between the usage of FFR and all-cause mortality in patients with stable angina undergoing PCI.

METHODS
Data was used from the SCAAR (Swedish Coronary Angiography and Angioplasty Registry) on all patients undergoing PCI (with or without FFR guidance) for stable angina pectoris in Sweden between January 2005 and March 2016. The primary endpoint was all-cause mortality, and the secondary endpoints were stent thrombosis (ST) or restenosis and peri-procedural complications. The primary model was multilevel Cox proportional hazards regression adjusted with Kernel-based propensity score matching.

RESULTS
In total, 23,860 patients underwent PCI for stable angina pectoris; of these, FFR guidance was used in 3,367. After a median follow-up of 4.7 years (range 0 to 11.2 years), the FFR group had lower adjusted risk estimates for all-cause mortality (hazard ratio: 0.81; 95% confidence interval [CI]: 0.73 to 0.89; p < 0.001), and ST and restenosis (hazard ratio: 0.74; 95% CI: 0.57 to 0.96; p = 0.022). The number of peri-procedural complications did not differ between the groups (adjusted odds ratio: 0.96; 95% CI: 0.77 to 1.19; p = 0.697).

CONCLUSIONS
In this observational study, the use of FFR was associated with a lower risk of long-term mortality, ST, and restenosis in patients undergoing PCI for stable angina pectoris. This study supports the current European and American guidelines for the use of FFR during PCI and shows that intracoronary pressure wire guidance confers prognostic benefit in patients with stable angina pectoris.

Völz|Sebastian|S| Dworeck|Christian|C| Redfors|Björn|B| Pétursson|Pétur|P| Angerås|Oskar|O| Gan|Li-Ming|LM| Götberg|Matthias|M| Sarno|Giovanna|G| Venetsanos|Dimitrios|D| Grimfärd|Per|P| Hofmann|Robin|R| Jensen|Jens|J| Björklund|Fredrik|F| Danielewicz|Mikael|M| Linder|Rickard|R| Råmunddal|Truls|T| Fröbert|Ole|O| Witt|Nils|N| James|Stefan|S| Erlinge|David|D| Omerovic|Elmir|E|

D053805:Fractional Flow Reserve, Myocardial

percutaneous coronary intervention
fractional flow reserve
stable angina pectoris
coronary artery disease


Telehealth amid the COVID-19 pandemic: perception among Asian, Native Hawaiian and Pacific Islander cancer patients.

Abstract: Aim: To assess the perception of telehealth visits among a multiracial cancer population during the coronavirus disease 2019 pandemic. Methods: This cross-sectional study was conducted at outpatient cancer clinics in Hawaii between March and August 2020. Patients were invited to participate in the survey either by phone or email. Results: Of the 212 survey respondents, 61.3% were Asian, 23.6% were White and 15.1% were Native Hawaiians or Pacific Islanders. Asians, Native Hawaiians and Pacific Islanders were less likely to desire future telehealth visits compared with Whites. Predictors with regard to preferring future telehealth visits included lower income and hematopoietic cancers. Conclusion: The authors found racial differences in preference for telehealth. Future studies aimed at overcoming these racial disparities are needed to provide equitable oncology care.

D044468:Native Hawaiian or Other Pacific Islander

telehealth
COVID-19
pandemic
health disparity
patient satisfaction
race
telemedicine
teleoncology


Enhanced cellulase production by Trichoderma harzianum by cultivation on glycerol followed by induction on cellulosic substrates.

Abstract: The use of glycerol obtained as an intermediate of the biodiesel manufacturing process as carbon source for microbial growth is a potential alternative strategy for the production of enzymes and other high-value bioproducts. This work evaluates the production of cellulase enzymes using glycerol for high cell density growth of Trichoderma harzianum followed by induction with a cellulosic material. Firstly, the influence of the carbon source used in the pre-culture step was investigated in terms of total protein secretion and fungal morphology. Enzymatic productivity was then determined for cultivation strategies using different types and concentrations of carbon source, as well as different feeding procedures (batch and fed-batch). The best strategy for cellulase production was then further studied on a larger scale using a stirred tank bioreactor. The proposed strategy for cellulase production, using glycerol to achieve high cell density growth followed by induction with pretreated sugarcane bagasse, achieved enzymatic activities up to 2.27 ± 0.37 FPU/mL, 106.40 ± 8.87 IU/mL, and 9.04 ± 0.39 IU/mL of cellulase, xylanase, and β-glucosidase, respectively. These values were 2 times higher when compared to the control experiments using glucose instead of glycerol. This novel strategy proved to be a promising approach for improving cellulolytic enzymes production, and could potentially contribute to adding value to biomass within the biofuels sector.

Delabona|Priscila da Silva|Pda S|
Lima|Deise Juliana|DJ|
Robl|Diogo|D|
Rabelo|Sarita Cândida|SC|
Farinas|Cristiane Sanchez|CS|
Pradella|José Geraldo da Cruz|JG|

Cellulases
Trichoderma harzianum
Biodiesel
Enzymes
Bioethanol
Gycerol
Pretreated bagasse


Validity of long-term and short-term recall of occupational sitting time in Finnish and Chinese office workers.

Abstract:
BACKGROUND
As sedentary behavior is a global health issue, there is a need for methods of self-reported sitting assessment. The accuracy and reliability of these methods should also be tested in various populations and different cultural contexts. This study examined the validity of long-term and short-term recall of occupational sitting time in Finnish and Chinese subgroups.

METHODS
Two cohort groups of office-based workers (58.6% female, age range 22-67 years) participated: a Finnish group (FIN, n = 34) and a Chinese group (CHI, n = 36). Long-term (past 3-month sitting) and short-term (daily sitting assessed on 5 consecutive days) single-item measures were used to assess self-reported occupational sitting time. Values from each participant were compared to objectively measured occupational sitting time assessed via thigh-mounted accelerometers, with Spearman's rho (ρ) used to assess validity and the Bland-Altman method used to evaluate agreement. Coefficients of variation depicted day-to-day variability of time spent on sitting at work.

RESULTS
In the total study sample, the results showed that both long-term and short-term recall correlated with accelerometer-derived sitting time (ρ = 0.532, 95% confidence intervals (CI): 0.336-0.684, p< 0.001; ρ = 0.533, 95%CI: 0.449-0.607, p< 0.001, respectively). Compared to objectively measured sitting time, self-reported occupational sitting time was 2.4% (95%CI: -0.5% to 5.3%, p = 0.091) and 2.2% (95%CI: 0.7%-3.6%, p = 0.005) greater for long-term and short-term recall, respectively. The agreement level was within the range -21.2% to 25.9% for long-term recall, and -24.2% to 28.5% for short-term recall. During a 5-day work week, day-to-day variation of sitting time was 9.4% ± 11.4% according to short-term recall and 10.4% ± 8.4% according to accelerometry-derived occupational sitting time.

CONCLUSION
Overall, both long-term and short-term self-reported instruments provide acceptable measures of occupational sitting time in an office-based workplace, but their utility at the individual level is limited due to large variability.

Gao|Ying|Y|
Cronin|Neil J|NJ|
Nevala|Nina|N|
Finni|Taija|T|

Validity
Sitting time
Office workers
Self-report
Accelerometry
Daily recall
Questionnaire


Simulations of skin and subcutaneous tissue loading in the buttocks while regaining weight-bearing after a push-up in wheelchair users.

Abstract: Pressure ulcers (PUs) are common in patients who chronically depend on a wheelchair for mobility, such as those with a spinal cord injury (SCI). In attempt to prevent the formation of PUs, pressure relieving maneuvers, such as push-ups, are commonly recommended for individuals with SCI. However, very little is known about skin and subcutaneous fat tissue load distributions during sitting and in particular their development during the process of regaining weight-bearing after a push-up. Knowledge on how these loads evolve during sitting-down is critical for understanding the susceptibility of skin to PUs. Considering the potential practical implications on guidelines for wheelchair users, we studied herein the build-up of shear loads in skin and subcutaneous fat using a model of the buttocks of a single SCI subject. Using 12 variants of our finite element (FE) model, we determined the shear loads in skin and subcutaneous fat tissues under the ischial tuberosities when sitting down on foam cushions with different stiffness properties, in healthy skin and scarred skin conditions, focusing on the time course of the build-up of tissue loads. We found substantial differences between the loading curves of skin and fat: While the fat was loaded at a nearly constant rate, skin loads increased nonlinearly - with a greater load/time slope at early skin-support contact. In the context of tissue health and prevention of PUs, this indicates that the more sensitive period with respect to skin integrity is at initial skin-support contact. We further found that the edges of a pre-existing scar are more susceptible to injury, and the greater risk for that is when a hypertrophic scar is present. Despite that this is a theoretical modeling study with associated limitations, we believe that it is already appropriate to recommend to patients to reposition themselves gradually and gently, and not to "fall" back into the wheelchair after finishing a push-up maneuver.

Pressure ulcer
Sitting
Cushion
Scar
Finite element model


Monitoring of the Sublingual Microcirculation During Cardiac Surgery: Current Knowledge and Future Directions.

Abstract: Handheld vital microscopes allow for direct observation of the sublingual microcirculatory perfusion during cardiac surgery. Through the use of handheld vital microscopes, it has been shown that cardiac surgery with cardiopulmonary bypass is associated with reduced and heterogenous microcirculatory perfusion. Microcirculatory impairment can result in inadequate tissue perfusion, leading to perioperative complications and poor outcome. Because microcirculatory impairment can occur despite stable or improved global hemodynamics, there is a yet unmet need for specific monitoring of the microcirculation. Technological advancements may facilitate point-of-care monitoring of microcirculatory perfusion using automated real-time analysis of microcirculatory measurements. Thus, microcirculatory monitoring may create new opportunities for specific microcirculatory treatment as part of hemodynamic management. The implementation of microcirculatory variables into personalized treatment concepts has the potential to improve hemodynamic management during cardiac surgery and thereby improve patient outcomes. Therefore, specific treatment strategies need to be developed to prevent or treat alterations of the microcirculatory perfusion. In the future, the use of handheld vital microscopes for microcirculatory monitoring may help to improve hemodynamic management and outcomes for patients undergoing cardiac surgical procedures.

Flick|Moritz|M|
Duranteau|Jacques|J|
Scheeren|Thomas W L|TWL|
Saugel|Bernd|B|

microcirculatory perfusion
cardiopulmonary bypass
microcirculatory monitoring
capillary perfusion
cardiothoracic surgery
microvascular flow index


Reviewing the review: a qualitative assessment of the peer review process in surgical journals.

Abstract:
Background
Despite rapid growth of the scientific literature, no consensus guidelines have emerged to define the optimal criteria for editors to grade submitted manuscripts. The purpose of this project was to assess the peer reviewer metrics currently used in the surgical literature to evaluate original manuscript submissions.

Methods
Manuscript grading forms for 14 of the highest circulation general surgery-related journals were evaluated for content, including the type and number of quantitative and qualitative questions asked of peer reviewers. Reviewer grading forms for the seven surgical journals with the higher impact factors were compared to the seven surgical journals with lower impact factors using Fisher's exact tests.

Results
Impact factors of the studied journals ranged from 1.73 to 8.57, with a median impact factor of 4.26 in the higher group and 2.81 in the lower group. The content of the grading forms was found to vary considerably. Relatively few journals asked reviewers to grade specific components of a manuscript. Higher impact factor journal manuscript grading forms more frequently addressed statistical analysis, ethical considerations, and conflict of interest. In contrast, lower impact factor journals more commonly requested reviewers to make qualitative assessments of novelty/originality, scientific validity, and scientific importance.

Conclusion
Substantial variation exists in the grading criteria used to evaluate original manuscripts submitted to the surgical literature for peer review, with differential emphasis placed on certain criteria correlated to journal impact factors.

Davis|Catherine H|CH|
Bass|Barbara L|BL|
Behrns|Kevin E|KE|
Lillemoe|Keith D|KD|
Garden|O James|OJ|
Roh|Mark S|MS|
Lee|Jeffrey E|JE|
Balch|Charles M|CM|
Aloia|Thomas A|TA|

Journal reviewer
Manuscript review
Surgery journals
Surgical manuscripts
Surgical research


Berberine hydrochloride attenuates voluntary methamphetamine consumption and anxiety-like behaviors via modulation of oxytocin receptors in methamphetamine addicted rats.

Abstract:
OBJECTIVE
Methamphetamine (METH) addiction is recognized as one of the major public health concerns, with no approved pharmacological agents for treatment. Berberine hydrochloride, an isoquinoline alkaloid in plants, induces antipsychotic and anxiolytic effects. Hence, we hypothesized that berberine may modulate the METH-induced rewarding effects.

MATERIALS AND METHODS
In this study, three groups of rat including control (N = 10), METH + vehicle (N = 10), and METH + berberine (N = 10) were kept in separate cages one day before expriments. METH (20 mg/L) was dissolved in tap water inside a bottle, while there was only tap water in the control bottle. Two groups received free METH solutions for two weeks (up to 12 mg/kg). Afterwards, they were abstianced for three weeks. Only one group received 100 mg/kg/day of berberine. After three weeks, locomotor activity and anxiety (elevated plus maze test) were evaluated, then the two-bottles choice model was used for one week to evaluate drug preferences. Finally, the brain of rats was removed for evaluation of oxytocin receptor expression via immunofluorescence staining method.

RESULTS
The results showed that METH preference was lower in the berberine + METH group during drug intake compared to the METH group (P < .05). During withdrawal, berberine reduced anxiety-like behaviors (P < .05) and decreased locomotor activity versus the METH group (P < .001). Also, berberine increased numbers of oxytocin receptors in comparison with the METH group (P < .01).

CONCLUSION
Considering the modulation of oxytocin receptors, berberine may be considered as a potential therapeutic agent for METH addiction.

Alavijeh|Mahnaz Mesripour|MM|
Vaezi|Gholamhassan|G|
Khaksari|Mehdi|M|
Hojati|Vida|V|

D000697:Central Nervous System Stimulants

Oxytocin receptor
Methamphetamine addiction
Anxiety
Drug preference
Berberine hydrocholoride


The effect of a bioactive tissue-engineered sling in a rat of stress incontinence model.

Abstract: In this study, we attempt to examine the feasibility of the bioactive tissue-engineered sling by using muscle precursor cells (MPCs)-seeded Poly(ε-caprolactone) (PCL) nanofiber sheet in a rat model of stress urinary incontinence (SUI). In vitro, MPCs were cultured on a PCL nanofiber sheet for one week, where the MPCs-seeded PCL nanofiber sheet showed constant twitching contraction by electrical field stimulation in an organ bath. In vivo, MPCs-seeded PCL nanofiber sheet was placed under the female rat's urethra after pudendal nerve denervation (animal model of SUI). The leak point pressure (LPP) was evaluated with the vertical tilt table after the operation for four weeks. The resulting LPP of MPCs-seeded PCL nanofiber sheet group was observed to be significantly higher than the denervation-only group's. Furthermore, PKH-26-labeled MPCs were observed under the urethral sphincter by immunohistochemistry. These results indicated that, the MPCs-seeded PCL nanofiber sheet have not only provided support for the deficient sphincter, but also actively improved the sphincter's function overall. In conclusion, this bioactive tissue-engineered sling could be used as an ideal material for the treatment of SUI.

Kim|In Gul|IG|
Piao|Shuyu|S|
Hong|Sung Hoo|SH|
Kim|Sae Woong|SW|
Hwang|Tae Kon|TK|
Oh|Se Heang|SH|
Lee|Jin Ho|JH|
Lee|Ji Youl|JY|

sling
muscle precursor cell
nanofiber
stress urinary incontinence
urethral sphincter


Racism as Public Health Crisis: Assessment and Review of Municipal Declarations and Resolutions Across the United States.

Abstract: Racism in the United States has been cited as a key driver of racial health inequities. Racism as a public health crisis has been in the forefront, particularly with respect to state and municipal governments that have developed legislation, resolutions, and declarations. This policy brief includes a review of resolutions and declarations across the US related to Racism as a Public Health Crisis through the end of September 2020. There were 125 resolutions reviewed for content related to the history of racism, reference to racial health equity data, content related to action steps or implementation, and any accompanying funding or resources. We found that the majority of policies name racism as critical in addressing racial inequities in health with limited details about specific actions, funding, or resources.

Mendez|Dara D|DD|
Scott|Jewel|J|
Adodoadji|Linda|L|
Toval|Christina|C|
McNeil|Monica|M|
Sindhu|Mahima|M|

D000069576:Health Equity
D006801:Humans
D009111:Local Government
D011634:Public Health
D044469:Racial Groups
D063505:Racism
D014481:United States

racism
public health
declarations
legislation
policy
health equity


Variability of allergens in commercial fish extracts for skin prick testing.

Abstract:
BACKGROUND
Commercial allergen extracts for allergy skin prick testing (SPT) are widely used for diagnosing fish allergy. However, there is currently no regulatory requirement for standardization of protein and allergen content, potentially impacting the diagnostic reliability of SPTs. We therefore sought to analyse commercial fish extracts for the presence and concentration of fish proteins and in vitro IgE reactivity using serum from fish-allergic patients.

METHODS
Twenty-six commercial fish extracts from five different manufacturers were examined. The protein concentrations were determined, protein compositions analysed by mass spectrometry, followed by SDS-PAGE and subsequent immunoblotting with antibodies detecting 4 fish allergens (parvalbumin, tropomyosin, aldolase and collagen). IgE-reactive proteins were identified using serum from 16 children with confirmed IgE-mediated fish allergy, with focus on cod, tuna and salmon extracts.

RESULTS
The total protein, allergen concentration and IgE reactivity of the commercial extracts varied over 10-fold between different manufacturers and fish species. The major fish allergen parvalbumin was not detected by immunoblotting in 6/26 extracts. In 7/12 extracts, five known fish allergens were detected by mass spectrometry. For cod and tuna, almost 70% of patients demonstrated the strongest IgE reactivity to collagen, tropomyosin, aldolase A or β-enolase but not parvalbumin.

CONCLUSIONS
Commercial fish extracts often contain insufficient amounts of important allergens including parvalbumin and collagen, resulting in low IgE reactivity. A comprehensive proteomic approach for the evaluation of SPT extracts for their utility in allergy diagnostics is presented. There is an urgent need for standardized allergen extracts, which will improve the diagnosis and management of fish allergy.

skin prick test
fish allergy
IgE reactivity
parvalbumin
allergy diagnostics


Associations Between Long-Term Gang Membership and Informal Social Control Processes, Drug Use, and Delinquent Behavior Among Mexican American Youth.

Abstract: Research has found that among juveniles weak ties to informal social control entities such as parents, school, and conventional peers increase the probability of the initiation and continuation of deviant behaviors such as drug use and crime. Given the weak ties of formal social control mechanisms in highly disadvantaged communities, informal social control mechanisms are often an important deterrent that reduce or moderate engagement in deviant behaviors among serious and persistent offenders. This analysis examines the association between long-term gang membership and adolescent informal social control processes, drug use, and delinquency. This research is based on data from a study of 160 Mexican American male gang members between the ages of 16 and 20. Findings suggest that among gang members in this context, commonly studied informal control mechanisms such as the family and schools do not function to deter long-term gang membership that is associated with serious criminal and violent behavior and drug use. The implications for future research on desistance or continuation of antisocial behavior across the life course are discussed.

Cepeda|Alice|A|
Saint Onge|Jarron M|JM|
Nowotny|Kathryn M|KM|
Valdez|Avelardo|A|

D000041:Absenteeism
D000293:Adolescent
D000294:Adolescent Behavior
D005195:Family Relations
D006801:Humans
D007604:Juvenile Delinquency
D017058:Mexican Americans
D010379:Peer Group
D012927:Social Control, Informal
D019966:Substance-Related Disorders
D013781:Texas
D055815:Young Adult

gangs
informal social control
drugs
deviant behaviors
juvenile delinquency


Dynamin-related protein 1: A critical protein in the pathogenesis of neural system dysfunctions and neurodegenerative diseases.

Abstract: Mitochondria play a key role in the maintenance of neuronal function by continuously providing energy. Here, we will give a detailed review about the recent developments in regards to dynamin-related protein 1 (Drp1) induced unbalanced mitochondrial dynamics, excessive mitochondrial division, and neuronal injury in neural system dysfunctions and neurodegenerative diseases, including the Drp1 knockout induced mice embryonic death, the dysfunction of the Drp1-dependent mitochondrial division induced neuronal cell apoptosis and impaired neuronal axonal transportation, the abnormal interaction between Drp1 and amyloid β (Aβ) in Alzheimer's disease (AD), the mutant Huntingtin (Htt) in Huntington's disease (HD), and the Drp1-associated pathogenesis of other neurodegenerative diseases such as Parkinson's disease (PD) and amyotrophic lateral sclerosis (ALS). Drp1 is required for mitochondrial division determining the size, shape, distribution, and remodeling as well as maintaining of mitochondrial integrity in mammalian cells. In addition, increasing reports indicate that the Drp1 is involved in some cellular events of neuronal cells causing some neural system dysfunctions and neurodegenerative diseases, including impaired mitochondrial dynamics, apoptosis, and several posttranslational modification induced increased mitochondrial divisions. Recent studies also revealed that the Drp1 can interact with Aβ, phosphorylated τ, and mutant Htt affecting the mitochondrial shape, size, distribution, axonal transportation, and energy production in the AD and HD neuronal cells. These changes can affect the health of mitochondria and the function of synapses causing neuronal injury and eventually leading to the dysfunction of memory, cognitive impairment, resting tremor, posture instability, involuntary movements, and progressive muscle atrophy and paralysis in patients.

Qi|Zhihao|Z| Huang|Zhen|Z| Xie|Feng|F| Chen|Linxi|L|0000-0002-2787-3018

dynamin-related protein 1
neural system dysfunctions
neurodegenerative diseases
mitochondrial dynamics


Low Radon Cleanroom for Underground Laboratories.

Abstract: Aim of a low radon cleanroom technology is to minimize at the same time radon, radon decay products concentration and aerosol concentration and to minimize deposition of radon decay products on the surfaces. The technology placed in a deep underground laboratory such as LSM Modane with suppressed muon flux and shielded against external gamma radiation and neutrons provides "Zero dose" space for basic research in radiobiology (validity of the LNT hypothesis for very low doses) and for the fabrication of nanoelectronic circuits to avoid undesirable "single event effects." Two prototypes of a low radon cleanroom were built with the aim to achieve radon concentration lower than 100 mBq·m3 in an interior space where only radon-free air is delivered into the cleanroom technology from a radon trapping facility. The first prototype, built in the laboratory of SÚRO Prague, is equipped with a standard filter-ventilation system on the top of the cleanroom with improved leakproofness. In an experiment, radon concentration of some 50 mBq·m-3 was achieved with the filter-ventilation system switched out. However, it was not possible to seal the system of pipes and fans against negative-pressure air leakage into the cleanroom during a high volume ventilation with the rate of 3,500 m3·h-1. From that reason more sophisticated second prototype of the cleanroom designed in the LSM Modane uses the filter-ventilation system which is completely covered in a further improved leakproof sealed metal box placed on the top of the cleanroom. Preliminary experiments carried out in the SÚRO cleanroom with a high radon activity injection and intensive filter-ventilation (corresponding to room filtration rate every 13 s) showed extremely low radon decay products equilibrium factor of 0.002, the majority of activity being in the form of an "unattached fraction" (nanoparticles) of 218Po and a surface deposition rate of some 0.05 mBq·m-2·s-1 per Bq·m-3. Radon exhalation from persons may affect the radon concentration in a low radon interior space. Balance and time course of the radon exhalation from the human body is therefore discussed for persons that are about to enter the cleanroom.

Štekl|Ivan|I| Hůlka|Jirí|J| Mamedov|Fadahat|F| Fojtík|Pavel|P| Čermáková|Eva|E| Jílek|Karel|K| Havelka|Miroslav|M| Hodák|Rastislav|R| Hýža|Miroslav|M|

radon
cleanroom
underground laboratory
zero dose
equilibrium factor
nanotechnology


The effect of itraconazole on the pharmacokinetics of lorlatinib: results of a phase I, open-label, crossover study in healthy participants.

Abstract: Background The third-generation tyrosine kinase inhibitor lorlatinib is approved for the treatment of ALK-positive metastatic NSCLC. CYP3A plays a major role in lorlatinib metabolism; therefore, a drug-drug interaction study was warranted to evaluate the impact of the strong CYP3A inhibitor, itraconazole, on lorlatinib plasma exposure. Methods This phase 1, open-label, 2-period, crossover study estimated the effects of itraconazole on the plasma pharmacokinetics and safety of lorlatinib in healthy participants (NCT02838264). Single-dose lorlatinib 50 mg (n = 2), 75 mg (n = 2) and 100 mg (n = 12) was administered in Period 1. In Period 2, itraconazole oral solution 200 mg/day was administered on Days 1-11, and single-dose lorlatinib on Day 5. Blood samples were collected up to 168 h after lorlatinib dosing. Results During daily dosing with itraconazole (Period 2), the ratios of the adjusted geometric means for area under the plasma concentration-time profile extrapolated to infinity (AUCinf) and maximum plasma concentration (Cmax) of single-dose lorlatinib 100 mg were 141.79% (90% confidence interval, 128.71%, 156.21%) and 124.39% (110.20%, 140.41%), respectively, compared with Period 1 (lorlatinib alone). Lorlatinib was well tolerated alone and with itraconazole. No serious adverse events or withdrawals were reported. Conclusions Co-administration of itraconazole and lorlatinib increased the plasma exposure of lorlatinib relative to lorlatinib alone in healthy participants. Therefore, concomitant use of lorlatinib with strong CYP3A inhibitors should be avoided. If this combination is unavoidable, the starting dose of lorlatinib should be reduced from 100 mg to 75 mg.

Patel|Maulik|M|
Chen|Joseph|J|
McGrory|Stephanie|S|
O'Gorman|Melissa|M|
Nepal|Sunil|S|
Ginman|Katherine|K|
Pithavala|Yazdi K|YK|

Itraconazole
Pharmacokinetics
Lorlatinib
Healthy participants
Drug-drug interaction
Phase 1 trial


CellCoal: Coalescent Simulation of Single-Cell Sequencing Samples.

Abstract: Our capacity to study individual cells has enabled a new level of resolution for understanding complex biological systems such as multicellular organisms or microbial communities. Not surprisingly, several methods have been developed in recent years with a formidable potential to investigate the somatic evolution of single cells in both healthy and pathological tissues. However, single-cell sequencing data can be quite noisy due to different technical biases, so inferences resulting from these new methods need to be carefully contrasted. Here, I introduce CellCoal, a software tool for the coalescent simulation of single-cell sequencing genotypes. CellCoal simulates the history of single-cell samples obtained from somatic cell populations with different demographic histories and produces single-nucleotide variants under a variety of mutation models, sequencing read counts, and genotype likelihoods, considering allelic imbalance, allelic dropout, amplification, and sequencing errors, typical of this type of data. CellCoal is a flexible tool that can be used to understand the implications of different somatic evolutionary processes at the single-cell level, and to benchmark dedicated bioinformatic tools for the analysis of single-cell sequencing data. CellCoal is available at https://github.com/dapogon/cellcoal.

D019143:Evolution, Molecular
D005821:Genetic Techniques
D005838:Genotype
D017422:Sequence Analysis, DNA
D059010:Single-Cell Analysis
D012984:Software

somatic evolution
allele dropout
amplification error
single-cell genomics


Nurses' interaction styles when supporting patients in self-management: A profile approach.

Abstract:
BACKGROUND
The rising attention to participation and self-regulation in chronic care requires nurses to move towards an approach in which patients' perspectives and choices are central, and in which patients' competency is fostered. According to Self-Determination Theory, nurses can differ in the way they interact with patients living with a chronic illness. That is, they can interact in an autonomy-supportive, controlling, structuring or chaotic way. However, in practice, nurses often use these styles side by side depending on personal and situational demands.

OBJECTIVE
Rooted in Self-Determination Theory, this study sought to identify distinct profiles among nurses involving the co-occurrence of autonomy support, structure, control and chaos (aim 1), and to examine whether such profiles are meaningfully driven by nurse-related indicators (aim 2).

DESIGN
A cross-sectional design with latent profile analysis.

METHODS
Data were collected using validated self-report questionnaires among nurses counselling chronically ill patients (N = 389). Latent profile analysis was performed to shed light on how nurses use different styles side by side; and subsequent MANCOVA testing was used to examine differences between the profiles in terms of nurse-related indicators.

RESULTS
Four profiles could be identified, each characterised by a unique combination of differing degrees of autonomy support, structure, control and chaos. The profiles included a motivating profile (20.31%) characterised by the dominant presence of autonomy support and structure; a demotivating chaotic profile (17.74%) characterised by the dominant presence of chaos; an active profile (24.17%) where all styles were highly present; and an undifferentiated profile (37.79%) characterised by an average presence of all styles. These four profiles were meaningfully related to a set of nurse-related indicators. Multivariate analysis (Pillai's Trace test = .38, F(15, 756) = 7.28; p < .001; η2 = .13) indicated that job competency, job autonomy and high-quality motivation were most elevated in the motivating profile.

CONCLUSION
Profiling has supported our understanding of the natural co-occurrence of more motivating and demotivating styles among nurses when counselling patients in self-managing their life with chronic illness. The pattern of retained profiles indicates that, for some nurses, it will be important to move away from controlling or chaotic interactions. Future intervention development should augment nurses' competence levels and high-quality motivation, with attention to reduce the pressure in nurses to act in a result-based manner. Profiling can also be valuable to better assign nurses to an employment in chronic care, and to support their personal professional growth.

Duprez|Veerle|V|
van der Kaap-Deeder|Jolene|J|
Beeckman|Dimitri|D|
Verhaeghe|Sofie|S|
Vansteenkiste|Maarten|M|
Van Hecke|Ann|A|

Nursing
Self-management
Self-Determination Theory
Latent profile analysis
Counselling
Patient participation
Patient-centred care


Modulation of olfactory-driven behavior by metabolic signals: role of the piriform cortex.

Abstract: Olfaction is one of the major sensory modalities that regulates food consumption and is in turn regulated by the feeding state. Given that the olfactory bulb has been shown to be a metabolic sensor, we explored whether the anterior piriform cortex (aPCtx)-a higher olfactory cortical processing area-had the same capacity. Using immunocytochemical approaches, we report the localization of Kv1.3 channel, glucose transporter type 4, and the insulin receptor in the lateral olfactory tract and Layers II and III of the aPCtx. In current-clamped superficial pyramidal (SP) cells, we report the presence of two populations of SP cells: glucose responsive and non-glucose responsive. Using varied glucose concentrations and a glycolysis inhibitor, we found that insulin modulation of the instantaneous and spike firing frequency are both glucose dependent and require glucose metabolism. Using a plethysmograph to record sniffing frequency, rats microinjected with insulin failed to discriminate ratiometric enantiomers; considered a difficult task. Microinjection of glucose prevented discrimination of odorants of different chain-lengths, whereas injection of margatoxin increased the rate of habituation to repeated odor stimulation and enhanced discrimination. These data suggest that metabolic signaling pathways that are present in the aPCtx are capable of neuronal modulation and changing complex olfactory behaviors in higher olfactory centers.

Piriform
Olfaction
Kv1.3
Glucose
Insulin
GLUT4
Sniffing behavior


Improving parallel imaging by jointly reconstructing multi-contrast data.

Abstract:
PURPOSE
To develop parallel imaging techniques that simultaneously exploit coil sensitivity encoding, image phase prior information, similarities across multiple images, and complementary k-space sampling for highly accelerated data acquisition.

METHODS
We introduce joint virtual coil (JVC)-generalized autocalibrating partially parallel acquisitions (GRAPPA) to jointly reconstruct data acquired with different contrast preparations, and show its application in 2D, 3D, and simultaneous multi-slice (SMS) acquisitions. We extend the joint parallel imaging concept to exploit limited support and smooth phase constraints through Joint (J-) LORAKS formulation. J-LORAKS allows joint parallel imaging from limited autocalibration signal region, as well as permitting partial Fourier sampling and calibrationless reconstruction.

RESULTS
We demonstrate highly accelerated 2D balanced steady-state free precession with phase cycling, SMS multi-echo spin echo, 3D multi-echo magnetization-prepared rapid gradient echo, and multi-echo gradient recalled echo acquisitions in vivo. Compared to conventional GRAPPA, proposed joint acquisition/reconstruction techniques provide more than 2-fold reduction in reconstruction error.

CONCLUSION
JVC-GRAPPA takes advantage of additional spatial encoding from phase information and image similarity, and employs different sampling patterns across acquisitions. J-LORAKS achieves a more parsimonious low-rank representation of local k-space by considering multiple images as additional coils. Both approaches provide dramatic improvement in artifact and noise mitigation over conventional single-contrast parallel imaging reconstruction. Magn Reson Med 80:619-632, 2018. © 2018 International Society for Magnetic Resonance in Medicine.

Bilgic|Berkin|B|
Kim|Tae Hyung|TH|
Liao|Congyu|C|0000-0003-2270-276X
Manhard|Mary Kate|MK|
Wald|Lawrence L|LL|
Haldar|Justin P|JP|
Setsompop|Kawin|K|

parallel imaging
virtual coil
GRAPPA
simultaneous multi-slice
LORAKS
partial Fourier


Methods for prevention of early childhood caries: Overview of systematic reviews.

Abstract: Early childhood caries (ECC) is a global problem, disproportionately affecting disadvantaged populations. The aim of this study was to evaluate systematically the available scientific evidence on the effectiveness of methods for ECC prevention. Six electronic databases were searched. Two independent reviewers selected the publications and analysed the quality of the included systematic reviews. Thirteen studies were included. Overall, eight reviews were classified with methodological quality critically low using the AMSTAR 2, whereas five reviews achieved a high risk of bias using the ROBIS tool. The methods identified that were positively related to the prevention of ECC were as follows: preventive dental programmes for pregnant women; advice on diet and feeding; prenatal oral health care; integration of maternal and children's oral health promotion into nursing practice; maternal oral health programmes undertaken by non-dental health professionals; dental health education in combination with the use of fluoride for children; early preventive dental visits; and the use of fluoride varnish and toothpastes with more than 1000 ppm of fluoride. The currently available evidence supporting the effectiveness of methods for prevention of ECC, although suggesting some methods of greater potential, is still lacking due to the methodological quality of the systematic reviews and the included primary studies.

oral health
child
dental caries
preventive methods


Overexpressed LAPTM4B-35 is a risk factor for cancer recurrence and poor prognosis in non-small-cell lung cancer.

Abstract:
BACKGROUND
The expression levels and clinical significances of Lysosomal-associated protein transmembrane-4β-35 (LAPTM4B-35) protein are unknown in the non-small-cell lung cancer (NSCLC). This study aimed to explore the expression and prognostic value of LAPTM4B-35 in NSCLC patients.

METHODS
The clinicopathological and survival data of 107 NSCLC patients who received radical surgery from 2007 and 2011 were reviewed. The LAPTM4B-35 expression of the paired tumors and adjacent normal specimens were detected, and the association between LAPTM4B-35 and clinical variables was explored. Kaplan-Meier analysis and Cox regression (Proportional hazard model) were performed to investigate the prognostic significance for NSCLC.

RESULTS
LAPTM4B-35 was over expressed in NSCLC tissues. The elevated LAPTM4B-35 expression was associated with cancer recurrence (P = 0.031). The 5-year median OS and PFS were significantly worse in the LAPTM4B-35 overexpressed group. Multivariate Cox analysis showed that LAPTM4B-35 over-expression was an independent factor for OS and PFS in NSCLC(P = 0.018, P = 0.026, respectively).

CONCLUSIONS
The overexpressed LAPTM4B-35 was an independent prognostic biomarker for NSCLC, which could predict cancer recurrence and poor over survival. And that may be applied as potential target for NSCLC treatment.

Kong|Fanming|F| Gao|Fangfang|F| Chen|Jun|J| Sun|Yiyu|Y| Zhang|Ying|Y| Liu|Honggen|H| Li|Xiaojiang|X| Yang|PeiYing|P| Zheng|Rongxiu|R| Liu|Geli|G| Jia|Yingjie|Y|

D015972:Gene Expression Regulation, Neoplastic

LAPTM4B-35
cancer recurrence
prognosis
non-small-cell lung cancer
lung cancer


Vaping associated lung injury: A potentially life-threatening epidemic in US youth.

Abstract:
BACKGROUND
The Center for Disease Control (CDC) has identified a national outbreak in the United States of over 2600 cases of e-cigarette or vaping product use-associated lung injury (EVALI), including 60 deaths as of January 2020. We describe our experience in six adolescents.

MATERIAL AND METHODS
We identified all pediatric patients diagnosed with EVALI by CDC guidelines over a 6-month period at our health system. Clinical presentation, hospital course, and imaging were reviewed.

RESULTS
Six patients were identified (three males, three females; median age 18.5 years). Presenting symptoms varied, including constitutional, gastrointestinal, neurologic, and respiratory complaints with pulmonary symptomatology becoming the dominant feature of the illness. Three patients required intensive care unit-level care, one of whom expired 36 days after presentation. Three had bronchoalveolar lavage, two with evidence of lipid-laden macrophages. Four had pulmonary function testing with various results. Admission chest radiographs in all revealed bibasilar interstitial infiltrate which rapidly progressed. Five patients had computed tomography chest imaging demonstrating: confluent pulmonary infiltrates with subpleural sparing (n = 2), generalized ground-glass opacities (n = 1), patchy ground-glass opacities (n = 1) and a reticulonodular pattern (n = 1). Brain magnetic resonance imaging (MRI) obtained in two patients was normal in one and showed a focal signal abnormality in the corpus callosum in one.

CONCLUSION
We describe the clinical course and radiologic findings of EVALI in our adolescent patients and present a new finding in the brain not yet described in the literature. Given the diversity of presenting symptoms, a high level of suspicion for EVALI is necessary for patients reporting vaping product use regardless of the presence of pulmonary complaints. Brain MRI should be strongly considered in patients with neurologic symptoms.

Messina|Mark D|MD|
Levin|Terry L|TL|
Conrad|Laura A|LA|
Bidiwala|Aneela|A|0000-0002-4881-368X

D066300:Electronic Nicotine Delivery Systems

e-cigarette or vaping associated lung injury


Effects of Patch-Matrix Composition and Individual Movement Response on Population Persistence at the Patch Level.

Abstract: Fragmentation creates landscape-level spatial heterogeneity which in turn influences population dynamics of the resident species. This often leads to declines in abundance of the species due to increased susceptibility to edge effects between the remnant habitat patches and the lower quality "matrix" surrounding these focal patches. In this paper, we formalize a framework to facilitate the connection between small-scale movement and patch-level predictions of persistence through a mechanistic model based on reaction-diffusion equations. The model is capable of incorporating essential information about edge-mediated effects such as patch preference, movement behavior, and matrix-induced mortality. We mathematically analyze the model's predictions of persistence with a general logistic-type growth term and explore their sensitivity to demographic attributes in both the patch and matrix, as well as patch size and geometry. Also, we provide bounds on demographic attributes and patch size in order for the model to predict persistence of a species in a given patch based on assumptions on the patch/matrix interface. Finally, we illustrate the utility of this framework with a well-studied planthopper species (Prokelisia crocea) living in a highly fragmented landscape. Using experimentally derived data from various sources to parameterize the model, we show that, qualitatively, the model results are in accord with experimental predictions regarding minimum patch size of P. crocea. Through application of a sensitivity analysis to the model, we also suggest a ranking of the most important model parameters based on which parameter will cause the largest output variance.

Minimum patch size
Habitat fragmentation
Matrix effects
Reaction diffusion equations


Argentine ants (Linepithema humile) use adaptable transportation networks to track changes in resource quality.

Abstract: Transportation networks play a crucial role in human and animal societies. For a transportation network to be efficient, it must have adequate capacity to meet traffic demand. Network design becomes increasingly difficult in situations where traffic demand can change unexpectedly. In humans, network design is often constrained by path dependency because it is difficult to move a road once it is built. A similar issue theoretically faces pheromone-trail-laying social insects; once a trail has been laid, positive feedback makes re-routing difficult because new trails cannot compete with continually reinforced pre-existing trails. In the present study, we examined the response of Argentine ant colonies and their trail networks to variable environments where resources differ in quality and change unexpectedly. We found that Argentine ant colonies effectively tracked changes in food quality such that colonies allocated the highest proportion of foragers to the most rewarding feeder. Ant colonies maximised access to high concentration feeders by building additional trails and routes connecting the nest to the feeder. Trail networks appeared to form via a pruning process in which lower traffic trails were gradually removed from the network. At the same time, we observed several instances where new trails appear to have been built to accommodate a surge in demand. The combination of trail building when traffic demand is high and trail pruning when traffic demand is low results in a demand-driven network formation system that allows ants to monopolise multiple dynamic resources.

Latty|Tanya|T|0000-0002-7469-8590
Holmes|Michael J|MJ|
Makinson|James C|JC|
Beekman|Madeleine|M|

Path dependency
Trails
Dynamic foraging
Pheromone trail


Genetics of Mitochondrial Disease.

Abstract: Mitochondria are intracellular organelles responsible for adenosine triphosphate production. The strict control of intracellular energy needs require proper mitochondrial functioning. The mitochondria are under dual controls of mitochondrial DNA (mtDNA) and nuclear DNA (nDNA). Mitochondrial dysfunction can arise from changes in either mtDNA or nDNA genes regulating function. There are an estimated ∼1500 proteins in the mitoproteome, whereas the mtDNA genome has 37 proteins. There are, to date, ∼275 genes shown to give rise to disease. The unique physiology of mitochondrial functioning contributes to diverse gene expression. The onset and range of phenotypic expression of disease is diverse, with onset from neonatal to seventh decade of life. The range of dysfunction is heterogeneous, ranging from single organ to multisystem involvement. The complexity of disease expression has severely limited gene discovery. Combining phenotypes with improvements in gene sequencing strategies are improving the diagnosis process. This chapter focuses on the interplay of the unique physiology and gene discovery in the current knowledge of genetically derived mitochondrial disease.

Mitochondrial disease
Mitochondrial DNA
Mitochondrial inheritance
Nuclear-encoded mitochondrial disease
Oxidative phosphorylation
mtDNA replication
mtDNA transcription
mtDNA translation
mtDNA-encoded disease


Ultrastructural and Phylogenetic Description of Kudoa orbicularis n. sp. (Myxosporea: Multivalvulida): A Parasite Infecting the Muscle of the Fish Chaetobranchopsis orbicularis (Teleostei: Cichlidae) in the Amazon Region.

Abstract: A new myxosporean species is described from the muscle of the Amazonian freshwater fish Chaetobranchopsis orbicularis (Teleostei, Cichlidae), with basis on morphometric, ultrastructural and molecular data. Numerous myxospores were observed within pseudocysts located on the hosts' dorsal and ventral muscles, near the neural spines and neural canal (spinal cord). Mature myxospores quadrangular with rounded ends in apical view, measuring 4.3 (3.6-5.0) μm in length and 5.1 (4.2-5.8) μm in width. The myxospores wall is formed by four symmetric valves. Within, four pyriform polar capsules, 2.1 (1.7-2.6) μm long and 1.3 (0.9-1.7) μm wide, located two by two in opposite sides of the myxospores longitudinal axis, each containing a polar filament forming 2-3 coils. Molecular analysis of the SSU rRNA gene by maximum likelihood, neighbor-joining and maximum parsimony confirms the parasite as a new member of the genus Kudoa, herein named Kudoa orbicularis n. sp., the second species of its genus reported from the South American freshwater fauna, and the fourth species worldwide known to occur in the freshwater environment. Furthermore, its sequence of the SSU rRNA gene constitutes the first entry of a freshwater Kudoa species in GenBank.

Azevedo|Carlos|C| Rocha|Sónia|S| Matos|Edilson|E| Oliveira|Elsa|E| Matos|Patrícia|P| Al-Quraishy|Saleh|S| Casal|Graça|G|

ultrastructure
freshwater
SSU rRNA gene
Brazil


Population genetics and ecological niche of invasive Aedes albopictus in Mexico.

Abstract: The Asian tiger mosquito Aedes albopictus (Skuse), is one of the most invasive mosquito species worldwide. In Mexico it is now recorded in 12 states and represents a serious public health problem, given the recent introduction of Chikungunya on the southern border. The aim of this study was to analyze the population genetics of A. albopictus from all major recorded foci, and model its ecological niche. Niche similarity with that from its autochthonous distribution in Asia and other invaded countries were analyzed and its potential future expansion and potential human exposure in climate change scenarios measured. We analyzed 125 sequences of a 317 bp fragment of the cyt b gene from seven A. albopictus populations across Mexico. The samples belong to 25 haplotypes with moderate population structuring (Fst=0.081, p<0.02) and population expansion. The most prevalent haplotype, found in all principal sites, was shared with the USA, Brazil, France, Madagascar, and Reunion Island. The ecological niche model using Mexican occurrence records covers 79.7% of the country, and has an 83% overlap with the Asian niche projected to Mexico. Both Neotropical and Nearctic regions are included in the Mexican niche model. Currently in Mexico, 38.6 million inhabitants are exposed to A. albopictus, which is expected to increase to 45.6 million by 2070. Genetic evidence supports collection information that A. albopictus was introduced to Mexico principally by land from the USA and Central and South America. Prevalent haplotypes from Mexico are shared with most invasive regions across the world, just as there was high niche similarity with both natural and invaded regions. The important overlap with the Asian niche model suggests a high potential for the species to disperse to sylvatic regions in Mexico.

Pech-May|Angélica|A|
Moo-Llanes|David A|DA|
Puerto-Avila|María Belem|MB|
Casas|Mauricio|M|
Danis-Lozano|Rogelio|R|
Ponce|Gustavo|G|
Tun-Ku|Ezequiel|E|
Pinto-Castillo|José Francisco|JF|
Villegas|Alejandro|A|
Ibáñez-Piñon|Clemente R|CR|
González|Cassandra|C|
Ramsey|Janine M|JM|

Population genetics
Ecological niche
Aedes albopictus
Mexico
Climate change
Invasive species


Involvement of caveolin-1 in low shear stress-induced breast cancer cell motility and adhesion: Roles of FAK/Src and ROCK/p-MLC pathways.

Abstract: Tumor cells translocating to distant sites are subjected to hemodynamic shear forces during their passage in the blood vessels. Low shear stress (LSS) plays a critical role in the regulation of various aspects of tumor cells functions, including motility and adhesion. Beyond its structural role, caveolin-1 (Cav-1), the important component of caveolae, represents a modulator of several cancer-associated functions as tumor progression and metastasis. However, the role of Cav-1 in regulating tumor cells response to shear stress remains poorly explored. Here, we characterized the role of LSS and Cav-1 in mediating cell motility and adhesion on human breast carcinoma MDA-MB-231 cells. We first showed that LSS exposure promoted cell polarity and focal adhesion (FA) dynamics, thus indicating elevated cell migration. Silencing of Cav-1 leaded to a significantly lower formation of stress fibers. However, LSS exposure was able to rescue it via the alteration of actin-associated proteins expression, including ROCK, p-MLC, cofilin and filamin A. Time-lapse migration assay indicated that Cav-1 expression fostered MDA-MB-231 cells motility and LSS triggered cells to rapidly generate new lamellipodia. Furthermore, Cav-1 and LSS significantly influenced cell adhesion. Taken together, our findings provide insights into mechanisms underlying LSS triggered events mediated by downstream Cav-1, including FAK/Src and ROCK/p-MLC pathways, involved in the reorganization of the cytoskeleton, cell motility, FA dynamics and breast cancer cell adhesion.

Xiong|Niya|N| Li|Shun|S| Tang|Kai|K| Bai|Hongxia|H| Peng|Yueting|Y| Yang|Hong|H| Wu|Chunhui|C| Liu|Yiyao|Y|

Caveolin-1
Cell motility
ROCK
Low shear stress
Focal adhesion
MLC


"Branched Tail" Oxyquinoline Inhibitors of HIF Prolyl Hydroxylase: Early Evaluation of Toxicity and Metabolism Using Liver-on-a-chip.

Abstract:
BACKGROUND
"Branched tail" oxyquinolines, and adaptaquin in particular, are potent HIF prolyl hydroxylase inhibitors showing promising results in in vivo hemorrhagic stroke models. The further improvement of the potency resulted in identification of a number of adaptaquin analogs. Early evaluation of toxicity and metabolism is desired right at the step of lead selection.

OBJECTIVE
The aim of the study is to characterize the toxicity and metabolism of adaptaquin and its new improved analogs.

METHOD
Liver-on-a-chip technology with differentiated HepaRG cells followed by LC-MS detection of the studied compounds and metabolites of the P450 substrate-inhibitor panel for CYP2B6, CYP2C9, CYP2C19, and CYP3A4.

RESULTS
The optimized adaptaquin analogs show no toxicity up to a 100-fold increased range over EC50. The drugs are metabolized by CYP3A4 and CYP2B6 as shown with the use of the cytochrome P450 substrate-inhibitor panel designed and optimized for preclinical evaluation of drugs' in vitro biotransformation on a 3D human histotypical cell model using "liver-on-a-chip" technology. Activation of CYP2B6 with the drugs tested has been observed. A scheme for adaptaquin oxidative conversion is proposed.

CONCLUSION
The optimized adaptaquin analogs are suitable for further preclinical trials. Activation of CYP2B6 with adaptaquin and its variants points to a potential increase in Tylenol toxicity if administered together.

Poloznikov|Andrey A|AA|
Nikulin|Sergey V|SV|
Zakhariants|Arpenik A|AA|
Khristichenko|Anna Y|AY|
Hushpulian|Dmitry M|DM|
Gazizov|Ildar N|IN|
Tishkov|Vladimir I|VI|
Gazaryan|Irina G|IG|

adaptaquin
HIF prolyl hydroxylase inhibitor
HepaRG cells
CYP2B6 induction
early drug-discovery stage
oxyquinolines.


Global shape integration and illusory form perception in the absence of awareness.

Abstract: Previous research on perceptual organization operations still provides contradictory evidence on whether the integration of sparse local elements into coherently unified shapes and the construction of the illusory form are accomplished without the need of awareness. In the present study, three experiments were conducted in which participants were presented with masked (Experiment 1, SOA=27ms; Experiment 2; SOA=53ms) and unmasked (Experiment 3) primes consisting of geometric shapes (a square or a diamond) that could be congruent or incongruent with subsequent probe stimuli (square vs. diamond). Furthermore, the primes were divided into: a grouping condition (where local elements may group together into global shapes), an illusory condition (where the arrangement of local elements produced illusory shapes) and a hybrid condition (where both operations were presented simultaneously). While no priming effects were found for the shortest SOA (27ms), both grouping and illusory primes produced significant priming effects in the longer SOA (53ms). On the other hand, results in Experiment 3 (unmasked) showed strong priming effects for the grouping of the inducers in both the grouping and the hybrid conditions, and also a significant but weaker priming effect for the illusory condition. Overall, our results support the possibility of the integration of local visual features into a global shape in the absence of awareness and, likewise, they suggest an early -subliminal- construction of the illusory shape, implying that feedback projections from higher to lower visual areas are not crucial in the construction of the illusory form.

Jimenez|Mikel|M|
Montoro|Pedro R|PR|
Luna|Dolores|D|

Illusory form
Awareness
Perceptual organization
Priming
Grouping
Subliminal


Physicochemical and Nutritional Requirements for Axenic Replication Suggest Physiological Basis for Coxiella burnetii Niche Restriction.

Abstract: Bacterial obligate intracellular parasites are clinically significant animal and human pathogens. Central to the biology of these organisms is their level of adaptation to intracellular replication niches associated with physicochemical and nutritional constraints. While most bacterial pathogens can adapt to a wide range of environments, severe niche restriction-an inability to thrive in diverse environments-is a hallmark of bacterial obligate intracellular parasites. Herein the physicochemical and nutritional factors underlying the physiological basis for niche restriction in the zoonotic bacterial obligate intracellular parasite and Q fever agent Coxiella burnetii are characterized. Additionally, these factors are reviewed in the context of C. burnetii evolution and continued (patho) adaptation. C. burnetii replication was strictly dependent on a combination of moderately acidic pH, reduced oxygen tension, and presence of carbon dioxide. Of macronutrients, amino acids alone support replication under physicochemically favorable conditions. In addition to utilizing gluconeogenic substrates for replication, C. burnetii can also utilize glucose to generate biomass. A mutant with a disruption in the gene pckA, encoding phosphoenolpyruvate carboxykinase (PEPCK), the first committed step in gluconeogenesis, could be complemented chemically by the addition of glucose. Disruption of pckA resulted in a moderate glucose-dependent growth defect during infection of cultured host cells. Although, C. burnetii has the theoretical capacity to synthesize essential core metabolites via glycolysis and gluconeogenesis, amino acid auxotrophy essentially restricts C. burnetii replication to a niche providing ample access to amino acids. Overall, the described combination of physiochemical and nutritional growth requirements are strong indicators for why C. burnetii favors an acidified phagolysosome-derived vacuole in respiring tissue for replication.

Vallejo Esquerra|Eduardo|E|
Yang|Hong|H|
Sanchez|Savannah E|SE|
Omsland|Anders|A|

Coxiella
niche restriction
obligate intracellular parasite
gluconeogenesis
axenic culture


Bioremoval of heavy metals and nutrients from sewage plant by Anabaena oryzae and Cyanosarcina fontana.

Abstract: The present study demonstrated the growth of two species of cyanobacteria on wastewater isolated from sewage plant in Aswan, Egypt. We evaluated their efficiency for eliminating nitrogen, phosphorus, chemical oxygen demand (COD) and heavy metals (Fe(2+), Pb(2+), Cu(2+), and Mn(2+)). The growth of Cyanosarcina fontana has supported wastewater as a growth medium than Anabaena oryzae compared to standard medium. The nutrients concentration such as COD, NO3-N and PO4-P were decreased by the growth of A. oryzae and C. fontana in the wastewater after primary settling and centrate. However, the reduction of COD was less efficient than the other nutrients. The reduction percentage of COD, NO3-N and PO4-P reached 39.3, 84.1 and 90.7% as well as 54.6, 83.1, and 89.8%, in cultures of A. oryzae and C. fontana grown in the wastewater after primary settling, respectively. The reduction amounted to 10.1, 76.8, and 63.0% by A. oryzae and 43.2, 62.1, and 74.8% by C. fontana, grown in the centrate, respectively. Cyanobacteria species have the ability to accumulate the heavy metals from the wastewater to level far than the exceeding metal level in the water. Whereas, the heavy metals biosorption performance of C. fontana was higher in accumulating Fe(2+) (93.95%), Pb(2+) (81.21%), Cu(2+) (63.9%), and Mn(2+) (48.49%) compared to A. oryzae. The biosorption ability is dependent on the nature of the adsorbent studied and the type of wastewater treated. Therefore, removal of heavy metals and nutrients by the tested algae is strongly recommended as a powerful technique for the removal of pollutants from wastewater.

D052918:Environmental Restoration and Remediation

heavy metals
Cyanosarcina fontana
bio-removal
sewage plant, Anabaena oryzae


The effect of preoperative phenazopyridine on short-term urinary retention following urogynecologic surgery.

Abstract:
INTRODUCTION AND HYPOTHESIS
Previous studies have found that administration of phenazopyridine decreased short-term urinary retention following surgery but other more recent trials have shown mixed results. This study sought to investigate the potential benefit of preoperative administration of oral phenazopyridine in relation to the prevention of short-term urinary retention following urogynecologic surgery.

METHODS
This is a retrospective cohort study of a convenience sample of women undergoing urogynecologic surgery from June 2016 to March 2019. Following surgery, subjects underwent a standardized retrograde voiding trial. The data had previously been gathered from a prior prospective trial at our institution (Kesty et al. Int Urogynecol J 31(9):1899-1905, 11). Chart review was performed to determine whether patients that received 200 mg of preoperative oral phenazopyridine to better visualize ureteral efflux during cystourethroscopy were more or less likely to pass their postoperative voiding trial. Bivariate statistical analysis was performed as well as a multivariate logistic regression model.

RESULTS
A total of 165 subjects were included in the final analysis; 100 who did not receive preoperative phenazopyridine and 65 who did receive phenazopyridine. There was no statistical difference between voiding trial pass rates following urogynecologic surgery between those who did not receive preoperative phenazopyridine compared to those who did [77% (77/100) and 82% (53/65), respectively, p = 0.37)]. The multivariate logistic regression model demonstrated no difference in postoperative voiding trial pass rates among those who received preoperative phenazopyridine compared to those who did not (OR 1.7, 95% CI: 0.53, 5.8).

CONCLUSIONS
Preoperative administration of oral phenazopyridine does not decrease short-term urinary retention following urogynecologic surgery.

Phenazopyridine
Void trial
Postoperative urinary retention


Precise Self-Assembly of Nanoparticles into Ordered Nanoarchitectures Directed by Tobacco Mosaic Virus Coat Protein.

Abstract: Self-assembly guided by biological molecules is a promising approach for fabricating predesigned nanostructures. Protein is one such biomolecule possessing deterministic 3D crystal structure and peptide information, which acts as a good candidate for templating functional nanoparticles (fNPs). However, inadequate coordination efficacy during the establishment of interfacial interactions with fNPs makes it highly challenging to precisely fabricate designed nanostructures and functional materials. Here, a facile and robust strategy is reported for the hierarchical assembly of fNPs into ordered architectures, with unprecedentedly large sizes up to tens of micrometers, using a hollow cylinder-shaped tobacco mosaic virus coat protein (TMV disk). The rational design of the site-specific functional groups on the TMV disk not only demonstrates the powerful capability of directing various discrete fNP assemblies with high controllability but also assists in precise assembly of a TMV monolayer sheet structure for further organizing homogeneous and heterogeneous fNP periodic lattices by varying the types of fNPs. The high precision and adjustability of the pattern fashions of different fNPs unambiguously corroborate the validity of this innovative strategy, which provides a convenient route to design and assemble protein-based hierarchical ordered architectures for use in nanophotonics and nanodevices.

D000596:Amino Acids
D001665:Binding Sites
D036022:Capsid Proteins
D053758:Nanoparticles
D049329:Nanostructures
D010316:Particle Size
D011485:Protein Binding
D014027:Tobacco Mosaic Virus
D014764:Viral Proteins

self-assembly
tobacco mosaic virus
interfacial interactions
nanoparticle lattices
protein structures


Dysfunctional RNA binding proteins and stress granules in multiple sclerosis.

Abstract: Dysfunction of the RNA binding protein (RBP) heterogeneous nuclear ribonuclear protein A1 (hnRNP A1) has been shown to contribute to the pathogenesis of neurodegenerative diseases, but its involvement in multiple sclerosis (MS) is largely unknown. In a neuronal cell line, interferon-γ caused hnRNP A1 nucleocytoplasmic mislocalization; colocalization of hnRNP A1 in stress granules (SGs); and inhibition of translation. Neurons in the brain of a MS patient showed pathogenic RBP dysfunction, including nuclear depletion of hnRNP A1, its mislocalization to the cytoplasm, and its colocalization in SGs. These data indicate a role for dysfunctional hnRNP A1 in the pathogenesis of MS.

Salapa|Hannah E|HE|
Johnson|Chloe|C|
Hutchinson|Catherine|C|
Popescu|Bogdan F|BF|
Levin|Michael C|MC|

D000076245:Heterogeneous Nuclear Ribonucleoprotein A1

Heterogeneous nuclear ribonucleoprotein A1


Successful Sequential Treatment with Itraconazole and ALA-PDT for Cutaneous Granuloma by Candida albicans: A Case Report and Literature Review.

Abstract: Photodynamic therapy (PDT) is a process that combines a photosensitizing drug and light and promotes phototoxic responses in target cells, mainly via oxidative damage. Antifungal photodynamic therapy has been successfully employed against Candida species, dermatophytes, and deep mycoses. We present a case of a cutaneous granuloma caused by C. albicans treated with 5-aminolevulinic acid (ALA)-PDT. A 64-year-old man presented with two plaques on his right hand and wrist for 2 years. The diagnosis was made based on histopathology, mycology, and molecular identification of paraffin-embedded tissues. The patient was treated with itraconazole for 1 month and two sessions of ALA-PDT. After 2 months of follow-up, the patient was cured and has not experienced any recurrence to date. ALA-PDT was well tolerated in this patient with little pain. In general, application of PDT in mycoses is safe and effective in most cases. ALA-PDT is a good choice for inactivation of C. albicans.

Cai|Qing|Q| Yang|Lian-Juan|LJ| Chen|Jia|J| Yang|Hong|H| Gao|Zhi-Qin|ZQ| Wang|Xiu-Li|XL|

ALA-PDT
Candida albicans
Infectious granuloma


Health disparity in CF: Perspectives from a lived experience.

Abstract: In this commentary, we discuss health disparities, reflecting on our experience in delayed diagnosis of cystic fibrosis based on race and bias in healthcare practitioners.


Bile Acid Conjugates with Anticancer Activity: Most Recent Research.

Abstract: The advantages of a treatment modality that combines two or more therapeutic agents in cancer therapy encourages the study of hybrid functional compounds for pharmacological applications. In light of this, we reviewed recent works on hybrid molecules based on bile acids. Due to their biological properties, as well as their different chemical/biochemical reactive moieties, bile acids can be considered very interesting starting molecules for conjugation with natural or synthetic bioactive molecules.

bile acids conjugates
anticancer agents
bioconjugation
drug design
natural product hybrids
pharmacological activity


Complications of proximal hypospadias repair with transverse preputial island flap urethroplasty: a 15-year experience with long-term follow-up.

Abstract: There is still debate regarding the optimal surgical approach for proximal hypospadias. This retrospective study aims to evaluate the long-term outcomes using transverse preputial island flap urethroplasty. A total of 320 patients were included, with a mean follow-up of 40.2 months (range: 1-156 months). Complications were encountered in 125 patients (39.1%), including fistulas in 53 (16.6%), urethral strictures in 31 (9.7%), and diverticula in 41 (12.8%). The mean timing of presentation with a complication was 15.8 months (median: 1.7, range: 1-145), of which 79.2% were early complications and 20.8% were late complications. In all, 20.8% of the patients with complications presented after ≥1 year, and 12.8% presented after ≥5 years. Univariate analysis revealed that age at the time of surgery, flap length, and location of the urethral meatus were not correlated with complications. A stricture was present in 31.7% (13/41) of those with diverticula (P < 0.001), while late urethral diverticula were accompanied by urethral strictures in 11.1% (1/9) of cases (P = 0.213). These results indicate that transverse preputial island flap urethroplasty still has a high incidence of complications, even when performed by highly experienced physicians. Most complications of hypospadias are diagnosed within 1 year postoperatively, while fistulas and urinary strictures generally occur within 2 months and diverticula tend to be present by 1 year.

D013521:Urologic Surgical Procedures, Male

hypospadias
follow-up
fistula
urethral stricture
late complications
diverticulum


The survey of the patient received the epiduroscopic laser neural decompression.

Abstract:
BACKGROUND
Neuroplasty using a Racz catheter or epiduroscope and percutaneous endoscopic laser discectomy are performed as treatment for chronic refractory low back and/or lower extremity pain, but they are limited in that they cannot completely remove the causing pathology. Lately, epiduroscopic laser neural decompression (ELND) has been receiving attention as an alternative treatment, but there are insufficient reports of results. Hence we aimed to investigate and report the data in our hospital.

METHODS
Seventy-seven patients were selected who had received ELND via the anterior and posterior epidural approach through the pain clinic in our hospital from March 2011 to July 2012. Their medical records including age, diagnosis, epiduroscopic findings and degree of symptom relief were investigated. The degree of symptom relief following the procedure was categorized into 5 stages of very good (5), good (4), no change (3), bad (2), and very bad (1) at 2 weeks and 1 month after the procedure.

RESULTS
The subjects were 30 males and 47 females. Mean age was 54.6 for males and 59.6 for females, so the overall mean age was 58.1 years old, with the youngest being 23 and the oldest 88 years old. In epiduroscopic images of all patients, more than one situation of herniated disc, fibrous tissue and adhesion, or inflammation was observed. Sixty-seven patients (87.0%) showed symptom relief 2 weeks after the procedure and 63 patients (81.8%) showed relief after 1 month.

CONCLUSIONS
ELND is considered to be an effective treatment alternative for chronic refractory low back and/or lower extremity pain, including lumbar disc herniation, lumbar spinal stenosis, and failed back surgery syndrome which cannot be alleviated with existing non-invasive conservative treatment.


Satisfaction with life among women with breast cancer - selected demographic and social factors.

Abstract:
OBJECTIVE
The aim of the study was evaluation of satisfaction with life among women ill with breast cancer, with consideration of selected demographic and social factors.

MATERIAL AND METHODS
In the study participated 121 patients from the Sub-Carpathian Oncology Centre in Brzozów, Poland, who had undergone surgical treatment due to breast cancer. The method of a diagnostic survey was used. The research instruments were an author-constructed questionnaire and the Satisfaction with Life Scale.

RESULTS
Women who received surgical treatment due to breast cancer evaluated their satisfaction with life on a mediocre level. Younger patients had a lower satisfaction with life than those who were older. Women living in urban areas evaluated their satisfaction with life in more positive terms than rural women. In turn, education level, marital status and material standard had no effect on the level of satisfaction with life among the women in the study.

CONCLUSIONS
According to the SWLS, women with the diagnosis of breast cancer obtained a mean result of 5.64 sten scores Younger women evaluated their satisfaction with life in more positive terms than those who were older. Women who lived in urban areas obtained a considerably higher result according to the SWLS, compared to rural inhabitants. Marital status, education level, material standard and occupational activity of the women were insignificant in the respondents' evaluation of satisfaction with life.

Cipora|Elżbieta|E|
Konieczny|Magdalena|M|
Karwat|Irena Dorota|ID|
Roczniak|Wojciech|W|
Babuśka-Roczniak|Magdalena|M|

satisfaction with life
breast cancer
demographic and social factors


Somatic support cells regulate germ cell survival through the Baz/aPKC/Par6 complex.

Abstract: Local signals and structural support from the surrounding cellular microenvironment play key roles in directing development in both embryonic organs and adult tissues. In Drosophila, male germ cells are intimately associated and co-differentiate with supporting somatic cells. Here, we show that the function of the Baz/aPKC/Par6 apical polarity complex in somatic cyst cells is required stage specifically for survival of the germ cells they enclose. Although spermatogonia enclosed by cyst cells in which the function of the Par complex had been knocked down survived and proliferated, newly formed spermatocytes enclosed by cyst cells lacking Par complex proteins died soon after onset of meiotic prophase. Loss of Par complex function resulted in stage-specific overactivation of the Jun-kinase (JNK) pathway in cyst cells. Knocking down expression of JNK pathway components or the GTPase Rab35 in cyst cells lacking Par complex function rescued the survival of neighboring spermatocytes, suggesting that action of the apical polarity complex ensures germ cell survival by preventing JNK pathway activation, and that the mechanism by which cyst cells lacking Par complex function kill neighboring spermatocytes requires intracellular trafficking in somatic cyst cells.

D048868:Adaptor Proteins, Signal Transducing

D047908:Intracellular Signaling Peptides and Proteins

Drosophila
Cyst cells
Par complex
JNK
Spermatogenesis