Just telling and selling: current limitations in the use of digital media in public health: a scoping review.

Abstract:
OBJECTIVE
To undertake a scoping review and to map research in the area of digital media use in public health.

STUDY DESIGN
Scoping review.

METHODS
PubMed, PsycINFO, Google and major textbooks of public health communication and health psychology were searched for primary studies or systematic reviews examining the use of digital media in a health context. Searches focussed on studies published between the start of 2000 and the end of June 2013. Abstracts of reviews of public health interventions were examined with respect to target groups, health topic, intervention characteristics, media used, study design, issues of quality and ethics, and outcomes. To map this area of work fully, this information was supplemented by adding information from primary studies. Areas were identified where systematic review evidence was scarce or non-existent by comparing the final map with information from the reviews analysed.

RESULTS
221 systematic reviews related to digital media use in a public health context were included. Most reviews included studies with an experimental design and general 'at risk' target populations. Specific settings were not specified in the majority of reviews. A large variety of health topics were covered. About a quarter of reviews did not specify a health topic but were concerned with broader issues of health promotion, disease prevention, or health education. Over half of the reviews focussed on eHealth and telemedicine, and another third were concerned with mass media - social marketing. Reviews most frequently reported behaviour-related outcomes or conducted some form of content analysis or analysis of the use of particular media. Research gaps were identified relating to community-based research, participation and empowerment, active media use (especially with respect to visual media und use of specific visual methodologies), and the use of salutogenic or assets-based approaches.

CONCLUSION
The available research relating to digital media use in public health is dominated by studies relating to eHealth, telehealth or social marketing; emphasising the passive reception of messages and a focus on individual behaviour change approaches. Issues of quality and ethics need to be taken into account more consistently. Further research is needed with respect to more participatory methods, particularly those which would seek to use digital media as a means to harness individual and community assets.

Clar|C|C| Dyakova|M|M| Curtis|K|K| Dawson|C|C| Donnelly|P|P| Knifton|L|L| Clarke|A|A|

Digital media
Public health
(Participatory) Visual methodologies


Network structure and functional properties of transparent hydrogel sanxan produced by Sphingomonas sanxanigenens NX02.

Abstract: The micro-network structure and functional properties of sanxan, a novel polysaccharide produced by Sphingomonas sanxanigenens NX02, were investigated. Transparent hydrogel sanxan was a high acyl polymer containing 8.96% acetyl and 4.75% glyceroyl. The micro-network structure of sanxan was mainly cyclic configurations composed of side-by-side intermolecular associations, with many rounded nodes found. Sanxan exhibited predominant gelation behavior at concentrations above 0.1%, which was enhanced by adding cations, especially Ca2+. The gel strength of sanxan was much higher than that of low acyl gellan, but slightly lower than that of high acyl gellan. Furthermore, the conformation transition temperature was increased in the presence of added cations. Moreover, sanxan showed excellent emulsifying and emulsion stabilizing properties. Consequently, such excellent functional properties make sanxan a good candidate as a gelling, stabilizing, emulsifying, or suspending agent in food and cosmetics industries, and in medical and pharmaceutical usage.

Wu|Mengmeng|M| Shi|Zhong|Z| Huang|Haidong|H| Qu|Jianmei|J| Dai|Xiaohui|X| Tian|Xuefeng|X| Wei|Weiying|W| Li|Guoqiang|G| Ma|Ting|T|

Sanxan
Micro-network structure
Emulsifying properties
Food additive
Viscoelastic properties


The Impact of Time Interval between Hepatic Resection and Liver Transplantation on Clinical Outcome in Patients with Hepatocellular Carcinoma.

Abstract: Hepatic resection (HR) for hepatocellular carcinoma (HCC) may require secondary liver transplantation (SLT). However, a previous HR is supposed to worsen post-SLT outcomes. Data of patients treated by SLT between 2000 and 2018 at two tertiary referral centers were analyzed. The primary outcome of the study was to analyze the impact of HR on post-LT complications. A Comprehensive Complication Index ≥ 29.6 was chosen as cutoff. The secondary outcome was HCC-related death by means of competing-risk regression analysis. In the study period, 140 patients were included. Patients were transplanted in a median of 23 months after HR (IQR 14-41). Among all the features analyzed regarding the prior HR, only time interval between HR and SLT (time HR-SLT) was an independent predictor of severe complications after LT (OR = 0.98, p < 0.001). According to fractional polynomial regression, the probability of severe complications increased up to 15 months after HR (43%), then slowly decreased over time (OR = 0.88, p < 0.001). There was no significant association between HCC-related death and time HR-SLT at the multivariable competing risks regression model (SHR, 1.06; 95% CI: 0.69-1.62, p = 0.796). This study showed that time HR-SLT was key in predicting complications after LT, without affecting HCC-related death.

Serenari|Matteo|M| Prosperi|Enrico|E| Allard|Marc-Antoine|MA| Paterno|Michele|M| Golse|Nicolas|N| Laurenzi|Andrea|A| Adam|René|R| Ravaioli|Matteo|M| Cherqui|Daniel|D| Cescon|Matteo|M|

liver transplantation
outcome
hepatocellular carcinoma
benchmark
hepatectomy


Cyclic-RGDfK peptide conjugated succinoyl-TPGS nanomicelles for targeted delivery of docetaxel to integrin receptor over-expressing angiogenic tumours.

Abstract:
UNLABELLED
Docetaxel (DTX) is an anticancer drug that is used alone and in combination with other drugs to treat tumours. However, it suffers from the drawback of non-specific cytotoxicity. To improve the therapeutic potential of DTX, we report the synthesis of cRGDfK peptide-conjugated succinoyl-TPGS (tocopheryl polyethylene glycol succinate) nanomicelles for targeted delivery of DTX. Among RGD (Arg-Gly-Asp) peptides, cRGDfK peptide shows specificity towards αvβ3 integrin receptors that are most commonly over-expressed in tumour cells. To cRGDfK peptide, succinoylated TPGS was synthesised and conjugated to cRGDfK peptide using a carbodiimide reaction. Peptide-conjugated DTX loaded nanomicelles (PDNM) displayed small particle size with a narrow distribution, controlled drug release and high physicochemical stability. Cytotoxicity, cellular uptake, apoptosis and anti-angiogenic comparisons of unconjugated nanomicelles to PDNM in DU145 human prostate cancer cells and HUVECs (Human Umblical Vein Endothelial Cells) clearly revealed the importance of the cRGDfK peptide in enhancing the drug delivery performance of nanomicelles.

FROM THE CLINICAL EDITOR
Common to many chemotherapeutic agents for cancer, systemic toxicity remains a big concern. In this article, the authors attempted to address this issue by conjugating RGD based peptides to Docetaxel, which would target integrins expressed on tumor cell surface. The experimental data revealed enhanced drug delivery.

Kulhari|Hitesh|H|
Pooja|Deep|D|
Shrivastava|Shweta|S|
Telukutala|Srinivasa R|SR|
Barui|Ayan K|AK|
Patra|Chitta Ranjan|CR|
Naidu Vegi|Ganga Modi|GM|
Adams|David J|DJ|
Sistla|Ramakrishna|R|

D061307:Human Umbilical Vein Endothelial Cells

Nanomicelles
Docetaxel
Cytotoxicity
cRGDfK
TPGS
Prostate cancer targeting


Is higher inequality less legitimate? Depends on How You Frame it!

Abstract: Economic inequality is increasing both globally and in various countries around the world, and such inequality has been linked to worsening health, well-being, and social cohesion. A key predictor for whether people take action against inequality is the extent to which they perceive it as illegitimate. We investigate how two variables jointly predict the legitimization of inequality, namely the perceived magnitude of differences in economic outcomes and the way these differences are described. Two experiments (total N = 190) tested whether framing the same difference in outcomes as an advantaged group having more or as a disadvantaged group having less moderates whether higher inequality is perceived as less legitimate. Participants perceived bigger differences as less legitimate when these differences were framed as the disadvantaged group having less. When they were framed as the advantaged group having more, the perceived magnitude of differences and legitimacy beliefs were unrelated. Together, this research highlights the importance of language for how people perceive and respond to inequality.

Bruckmüller|Susanne|S|
Reese|Gerhard|G|
Martiny|Sarah E|SE|

framing
economic inequality
comparison
income inequality
social justice


Incidence and risk factors for acute delirium in older patients with a hip fracture: A retrospective cohort study.

Abstract: This retrospective cohort study aimed to determine the incidence, and preoperative, intraoperative, and postoperative risk factors for postoperative delirium in older patients undergoing surgical fixation of a hip fracture. Electronic medical records were examined of 260 patients who underwent a surgical fixation of a hip fracture between June 2017 and October 2018 at a university-affiliated tertiary care hospital in Victoria, Australia. Demographic, clinical, and perioperative data were examined for potential risk factors for postoperative delirium. Of the 260 patients, 63 patients (24.2%) developed delirium postoperatively. Univariate logistic regression analysis indicated that advanced age, comorbidity, cognitive impairment, dementia, American Society of Anesthesiologists score, and antipsychotic usage were significant risk factors for delirium, while doses of paracetamol, fentanyl, and diazepam showed complex associations. Multivariate logistic regression analysis determined comorbidity and cognitive impairment as independent risk factors for the development of delirium. This study demonstrates the importance of evaluation of medications prescribed in the perioperative period as modifiable risk factors, in order to identify patients at high risk of delirium and enable targeted monitoring and treatment during patients' hospitalization.

incidence
risk factors
hip fracture
postoperative delirium


Exploring the Protective Potential of Carboxymethyl Terminalia catappa Polysaccharide on Blue Light Light-Emitting Diode Induced Corneal Damage.

Abstract:
BACKGROUND
Excessive blue light light-emitting diode (LED) exposure and consequent oxidative stress causes corneal damage and corneal injuries are the major problem arising these days due to excessive use of mobile phone, TV, environment pollution, etc. Objective: In the present investigation, the protectiveness of carboxymethyl Terminalia catappa (CTC) from blue light LED-induced corneal damage was explored.

METHODS
For this purpose, Terminalia catappa (TC) was functionalized by carboxymethylation and its structural modification was confirmed by spectral attributes. Further, the CTC protective eye drop formulations (0.025-1%, w/v) were prepared and evaluated for their capability of protection from blue light LEDinduced corneal damage as compared to CTC protective eye gel (1.25-7%, w/v). The findings pointed towards excellent protection of CTC gel formulations as compared to CTC eye drop formulations. In addition, the prepared optimized CTC gel had thixotropic behavior as evident from percentage structural recovery which was 1.75 fold higher than marketed formulation (I-Comfort, HPMC 2%, w/v). The safety and non-toxicity of CTC protective eye drop and gel were confirmed by HET-CAM test. Further, a rat eye model was implemented that mimic blue light light-emitting diode induced corneal damage in day to day life to assess the protective effect of CTC protective eye drop and gel.

RESULTS
The order of protectiveness of CTC formulations was found to be CTC protective eye gel (4%, w/v) (no corneal damage)>marketed eye gel (12.34% corneal damage)=CTC protective eye drop (0.75%, w/v) (17.48% corneal damage)> marketed eye drop (51% corneal damage). The mechanism behind the protective effect of CTC eye drop and gel was associated with good free radical scavenging activity and corneal adhesive property of CTC. It is established from the present work that, carboxymethyl Terminalia catappa has protective action against blue light light-emitting diode induced corneal damage.

carboxymethylation
Terminalia catappa
blue light
corneal damage
eye drop
eye gel
Light Emitting Diode (LED)
protective effect.


Waste-based alternative adsorbents for the remediation of pharmaceutical contaminated waters: Has a step forward already been taken?

Abstract: When adsorption is considered for water treatment, commercial activated carbon is usually the chosen adsorbent for the removal of pollutants from the aqueous phase, particularly pharmaceuticals. In order to decrease costs and save natural resources, attempts have been made to use wastes as raw materials for the production of alternative carbon adsorbents. This approach intends to increase efficiency, cost-effectiveness, and also to propose an alternative and sustainable way for the valorization/management of residues. This review aims to provide an overview on waste-based adsorbents used on pharmaceuticals' adsorption. Experimental facts related to the adsorption behaviour of each adsorbent/pharmaceutical pair and some key factors were addressed. Also, research gaps that subsist in this research area, as well as future needs, were identified. Simultaneously, this review aims to clarify the current status of the research on pharmaceuticals' adsorption by waste-based adsorbents in order to recognize if the right direction is being taken.

Silva|Carla Patrícia|CP|
Jaria|Guilaine|G|
Otero|Marta|M|
Esteves|Valdemar I|VI|
Calisto|Vânia|V|

Pharmaceuticals
Adsorption
Activated carbon
Waste management
Wastewater treatment


Induction of foetal haemoglobin synthesis in erythroid progenitor stem cells: mediated by water-soluble components of Terminalia catappa.

Abstract: Current novel therapeutic agents for the treatment of sickle cell anaemia (SCA) focus on increasing foetal haemoglobin (HbF) levels in SCA patients. Unfortunately, the only approved HbF-inducing agent, hydroxyurea, has long-term unpredictable side effects. Studies have shown the potential of plant compounds to modulate HbF synthesis in primary erythroid progenitor stem cells. We isolated a novel HbF-inducing Terminalia catappa distilled water active fraction (TCDWF) from Terminalia catappa leaves that induced the commitment of erythroid progenitor stem cells to the erythroid lineage and relatively higher HbF synthesis of 9.2- and 6.8-fold increases in both erythropoietin (EPO)-independent and EPO-dependent progenitor stem cells respectively. TCDWF was differentially cytotoxic to EPO-dependent and EPO-independent erythroid progenitor stem cell cultures as revealed by lactate dehydrogenase release from the cells. TCDWF demonstrated a protective effect on EPO-dependent and not EPO-independent progenitor cells. TCDWF induced a modest increase in caspase 3 activity in EPO-independent erythroid progenitor stem cell cultures compared with a significantly higher (P˂0.05) caspase 3 activity in EPO-dependent ones. The results demonstrate that TCDWF may hold promising HbF-inducing compounds, which work synergistically, and suggest a dual modulatory effect on erythropoiesis inherent in this active fraction.

Aimola|I A|IA|
Inuwa|H M|HM|
Nok|A J|AJ|
Mamman|A I|AI|

foetal haemoglobin
erythroid progenitor stem cells
Terminalia catappa
sickle cell anaemia
differentiation
proliferation
β-thalassemia


Evolutionary and functional substitution of extrinsic musculature in Solifugae (Arachnida).

Abstract: The locomotory system of Solifugae is distinct from that of other Arachnida in several ways. Only three pairs of legs are involved in locomotion, while the first pair function as sensory appendages. Morphologically, the proximal region of the locomotory system in Solifugae is characterized by fused coxae. Within the prosoma of Solifugae, an endosternite is missing: in other Arachnida, this endosternite serves as the proximal attachment site for a portion of the extrinsic musculature. How then do these skeletal modifications influence the muscular anatomy in the proximal region of the locomotory system? To answer this question, we studied the skeletomuscular anatomy of Galeodes granti at the interface between the prosoma and legs, reinvestigating the complex muscular anatomy of this body region for the first time in over 80 years and-for the first time-using detailed micro-computed tomography scans to analyze the skeletomuscular morphology. Specimens of three further species were checked for comparison. The analysis revealed differences in the number and composition of coxa-trochanter muscles in each of the four pairs of legs. These are compared in the light of serial homology. The comparison between the proximal locomotory system of Solifugae and that of other Arachnida unveils a series of analogies. Primarily, the coxa-trochanter joint is the most proximal joint to move the leg relative to the prosoma. Therefore, we argue that from a morpho-functional point of view, the coxa-trochanter muscles in Solifugae should be considered secondary extrinsic musculature. Thus, the legs gain a stable, articulated joint in the most proximal region of the leg to the prosoma, which might be advantageous for agile locomotion.

coxa
endosternite
intrinsic muscles
trochanter


Segmentation and visualization of the human cranial bone by T2* approximation using ultra-short echo time (UTE) magnetic resonance imaging.

Abstract: Segmentation of the human cranial bone from MRI data is challenging, because compact bone is characterized by very short transverse relaxation times and typically produces no signal when using conventional magnetic resonance imaging (MRI) sequences. In this work, we propose a fully automated segmentation algorithm, which uses dual-echo, ultra-short echo-time (UTE) MRI data. The segmentation was initialized by interval thresholding of approximated T2* relaxation time maps in the range of 1ms<T2*<3ms. This parameter range was derived from a manual region-of-interest analysis of high resolution data of the cranial layers, resulting in average T2* relaxation times of 1.7±0.3ms in the lamina externa, 2.5±0.3ms in the diploe and 1.7±0.2ms in the lamina interna. Segmentations were performed based on data of 8 healthy volunteers that were acquired with different acquisition parameters and spatial resolutions to test the stability of the algorithm. Comparison with computed tomography data demonstrated close agreement with the segmented UTE MRI data. Visualization of the segmented cranial bone was performed by volumetric rendering and by using the approximated T2* values for color coding, clearly visualizing the cranial sutures as well as their intersections.

Krämer|Martin|M|
Herzau|Benedikt|B|
Reichenbach|Jürgen R|JR|


Molecular evidence for a recent demographic expansion in the puma (Puma concolor) (Mammalia, Felidae).

Abstract: The puma is an iconic predator that ranges throughout the Americas, occupying diverse habitats. Previous phylogeographic analyses have revealed that it exhibits moderate levels of genetic structure across its range, with few of the classically recognized subspecies being supported as distinct demographic units. Moreover, most of the species' molecular diversity was found to be in South America. To further investigate the phylogeographic structure and demographic history of pumas we analyzed mtDNA sequences from 186 individuals sampled throughout their range, with emphasis on South America. Our objectives were to refine the phylogeographic assessment within South America and to investigate the demographic history of pumas using a coalescent approach. Our results extend previous phylogeographic findings, reassessing the delimitation of historical population units in South America and demonstrating that this species experienced a considerable demographic expansion in the Holocene, ca. 8,000 years ago. Our analyses indicate that this expansion occurred in South America, prior to the hypothesized re-colonization of North America, which was therefore inferred to be even more recent. The estimated demographic history supports the interpretation that pumas suffered a severe demographic decline in the Late Pleistocene throughout their distribution, followed by population expansion and re-colonization of the range, initiating from South America.

Matte|Eunice M|EM|
Castilho|Camila S|CS|
Miotto|Renata A|RA|
Sana|Denis A|DA|
Johnson|Warren E|WE|
O'Brien|Stephen J|SJ|
de Freitas|Thales R O|TR|
Eizirik|Eduardo|E|

conservation genetics
mammals
mitochondrial DNA
molecular time estimate
phylogeography


Association of LEPR polymorphisms with egg production and growth performance in female Japanese quails.

Abstract: This study aimed to screen intron 8 of the leptin receptor (LEPR) gene for polymorphisms in female Japanese quails. Two adjacent novel SNPs (A277G and A304G) were detected using PCR-SSCP and sequencing. These SNPs produced three haplotypes (AA/AA, AG/AG, and GG/GG) that were significantly (p ≤ 0.05) associated with growth and egg production traits. GG/GG haplotype-quails had significantly (p ≤ 0.05) lower egg production, feed intake, growth performance, lipid profile, serum levels of sex hormones (estradiol, progesterone, FSH, LH), and ovarian expressions of survivin, FSHR, and IGF1 than other quails. However, GG/GG quails had significantly (p ≤ 0.05) higher serum levels of LEP and mRNA levels of LEPR, LEP, and caspase 3 in the hypothalamus and ovaries. These higher levels of LEP/LEPR could not only reduce feed intake and body weight gain but also could induce apoptosis of ovarian cells (as indicated by lower survivin and IGF1 and higher caspase3 expression) which could inhibit the development of the follicles and the release of sex hormones with a subsequent decrease in egg production in GG/GG quails. Therefore, with these results, we suggest selecting Japanese quails with AA/AA and AG/AG haplotypes to improve the reproduction and growth performance of this flock.

LEPR
egg production
SNPs
LEP
growth traits


Generation of spatially aligned collagen fiber networks through microtransfer molding.

Abstract: The unique biomechanical properties of native tissue are governed by the organization and composition of integrated collagen and elastin networks. An approach for fabricating spatially aligned, fiber-reinforced composites with adjustable collagen fiber dimensions, layouts, and distribution within an elastin-like protein matrix yielding a biocomposite with controllable mechanical responses is reported. Microtransfer molding is employed for the fabrication of hollow and solid collagen fibers with straight or crimped fiber geometries. Collagen fibers (width: 2-50 μm, thickness: 300 nm to 3 μm) exhibit a Young's modulus of 126 ± 61 MPa and an ultimate tensile strength of 7 ± 3.2 MPa. As fiber networks within composite structures, straight fiber layouts display orthotropic responses with Young's modulus ranging from 0.95 ± 0.35 to 10.4 ± 0.5 MPa and tensile strength from 0.22 ± 0.08 to 0.87 ± 0.5 MPa with increasing fraction of collagen fibers (1-10%, v/v). In contrast, composites based on crimped fiber layouts exhibit strain-dependent stiffness with an increase in Young's modulus from 0.7 ± 0.14 MPa to 3.15 ± 0.49 MPa, at a specific transition strain. Through controlling the microstructure of engineered collagen fiber networks, a facile means is established to control macroscale mechanical responses of composite protein-based materials.

Naik|Nisarga|N|
Caves|Jeffrey|J|
Chaikof|Elliot L|EL|
Allen|Mark G|MG|

D000818:Animals
D001672:Biocompatible Materials
D001696:Biomechanical Phenomena
D003094:Collagen
D055119:Elastic Modulus
D004549:Elastin
D004926:Escherichia coli
D005109:Extracellular Matrix
D051381:Rats
D017207:Rats, Sprague-Dawley
D011994:Recombinant Proteins
D013718:Tensile Strength
D023822:Tissue Engineering

bio-MEMS
collagen microfibers
elastin-mimetic protein polymer
fiber reinforced composite


Incidence and clinical relevance of left atrial appendage membranes: a new congenital heart disease?

Abstract:
AIMS
Left atrial appendage (LAA) membranes are rare congenital anomalies. Those involving the appendage orifice may obstruct its emptying flows, thus promoting blood stasis and clot formation. However, the epidemiology of LAA membranes has never been studied and a correlation with appendage thrombosis has never been proved. Very few case reports described LAA membranes, therefore, their frequency and clinical significance are not known. Moreover, their presence and degree are of crucial importance in planning LAA percutaneous closure, a procedure whose indication is evolving, and whether their presence can represent technical issues during the device implantation is not known. This study aimed to evaluate the incidence and the clinical significance of LAA membranes.

METHODS AND RESULTS
A population of 6030 consecutive transoesophageal echo (TOE) studies has been retrospectively reviewed in order to find those patients in whom an LAA membrane has been found. A literature research has been performed to review previous described cases. Among 6030 TOE cases, an LAA membrane has been described in 6 (prevalence of 1/1000). In one case, the membrane was associated to a severe LAA hypoplasia and in another case to an LAA thrombus (these represent the first cases ever described). All patients had an atrial fibrillation (AF) history and two were in AF during the TOE exam.

CONCLUSION
LAA membranes are rare congenital abnormalities occasionally discovered during a TOE exam, frequently in patients affected by AF. In half of the cases, they obstruct the LAA flow, thus theoretically pre-disposing to clot formation. They may be rarely associated to an appendage hypoplasia. During a TOE exam, cardiac imagers should always rule out their presence.

Cresti|Alberto|A|0000-0003-2328-8943
Solari|Marco|M|
Gismondi|Anna Laura|AL|
Baratta|Pasquale|P|
De Sensi|Francesco|F|
Breschi|Marco|M|
Limbruno|Ugo|U|

left atrial appendage
percutaneous closure
atrial fibrillation
atrial membranes
atrial thrombosis


Event-related potentials to rare visual targets and negative symptom severity in a transdiagnostic psychiatric sample.

Abstract:
OBJECTIVES
Negative psychiatric symptoms are often resistant to treatments, regardless of the disorder in which they appear. One model for a cause of negative symptoms is impairment in higher-order cognition. The current study examined how particular bottom-up and top-down mechanisms of selective attention relate to severity of negative symptoms across a transdiagnostic psychiatric sample.

METHODS
The sample consisted of 130 participants: 25 schizophrenia-spectrum disorders, 26 bipolar disorders, 18 unipolar depression, and 61 nonpsychiatric controls. The relationships between attentional event-related potentials following rare visual targets (i.e., N1, N2b, P2a, and P3b) and severity of the negative symptom domains of anhedonia, avolition, and blunted affect were evaluated using frequentist and Bayesian analyses.

RESULTS
P3b and N2b mean amplitudes were inversely related to the Positive and Negative Syndrome Scale-Negative Symptom Factor severity score across the entire sample. Subsequent regression analyses showed a significant negative transdiagnostic relationship between P3b amplitude and blunted affect severity.

CONCLUSIONS
Results indicate that negative symptoms, and particularly blunted affect, may have a stronger association with deficits in top-down mechanisms of selective attention.

SIGNIFICANCE
This suggests that people with greater severity of blunted affect, independent of diagnosis, do not allocate sufficient cognitive resources when engaging in activities requiring selective attention.

Salgari|Giulia C|GC|
Potts|Geoffrey F|GF|
Schmidt|Joseph|J|
Chan|Chi C|CC|
Spencer|Christopher C|CC|
Bedwell|Jeffrey S|JS|

Event-related potentials
Negative symptoms
Transdiagnostic
Selective attention
Schizophrenia-spectrum disorders
Mood disorders


A phenomenological spiking model for octopus cells in the posterior-ventral cochlear nucleus.

Abstract: Octopus cells in the posteroventral cochlear nucleus exhibit characteristic onset responses to broad band transients but are little investigated in response to more complex sound stimuli. In this paper, we propose a phenomenological, but biophysically motivated, modeling approach that allows to simulate responses of large populations of octopus cells to arbitrary sound pressure waves. The model depends on only few parameters and reproduces basic physiological characteristics like onset firing and phase locking to amplitude modulations. Simulated responses to speech stimuli suggest that octopus cells are particularly sensitive to high-frequency transients in natural sounds and their sustained firing to phonemes provides a population code for sound level.

Octopus cells
Natural sounds
Auditory brainstem


Exploration of Different Rehabilitation Routes for Sepsis Survivors with Monitoring of Health Status and Quality of Life: RehaSep Trial Protocol.

Abstract:
INTRODUCTION
This project addresses the important problem of sepsis sequelae resulting in frequent hospital readmissions and higher mortality rate in the post-discharge period. However, neither specific diagnostic methods nor standards for rehabilitation of sepsis patients have been introduced yet. The aim of this study is to evaluate the effectiveness of two different multiparameter-monitored rehabilitation treatments in order to improve the health status and quality of life of sepsis survivors.

METHODS
Decades of failed randomized controlled trials involving sepsis patients strongly suggest the need for a paradigm change. Therefore, we designed a prospective, interventional, controlled, pragmatic, patient-centred trial based on the principles of personalized medicine. Sixty post-sepsis patients after hospital discharge will be individually assigned to a control group (without intervention) and two groups with 3-month diagnostically monitored rehabilitation programs based either on the recumbent cycloergometer training or on the experimental hyperbaric oxygen therapy. In all of the patients a wide range of physiological (spirometry, ECG/cycloergometer exercise test), haematological (microscopy) and biochemical (blood tests) parameters will be assessed at hospital discharge and during subsequent 3 months in order to monitor changes of their physical capacity, immunity and degree of post-sepsis organ damage/recovery. For quality of life monitoring a novel tool-"Life After Sepsis Survey"-will be applied.

PLANNED OUTCOMES
A set of composite quantitative indices resulting from laboratory measurement data combined with the quality of life questionnaire data will constitute the primary outcomes whereas mortality rate and hospital readmission number will be counted as the secondary outcomes.

CONCLUSIONS
Critical analysis of past trials prompted us to implement multiple improvements in tools and procedures. The results of this trial will contribute to the development of rehabilitation therapy addressing not only weakness but also organ damage problems of sepsis survivors.

TRIAL REGISTRATION
ANZCTR ( http://www.anzctr.org.au ): ACTRN12618000347268, U1111-1210-6110.

FUNDING
This research was funded by the National Science Center, Poland.

Rehabilitation
Sepsis survivors
Quality of life
Hyperbaric oxygen therapy
Infectious diseases
PICS
Post-sepsis care


From iconic handshapes to grammatical contrasts: longitudinal evidence from a child homesigner.

Abstract: Many sign languages display crosslinguistic consistencies in the use of two iconic aspects of handshape, handshape type and finger group complexity. Handshape type is used systematically in form-meaning pairings (morphology): Handling handshapes (Handling-HSs), representing how objects are handled, tend to be used to express events with an agent ("hand-as-hand" iconicity), and Object handshapes (Object-HSs), representing an object's size/shape, are used more often to express events without an agent ("hand-as-object" iconicity). Second, in the distribution of meaningless properties of form (morphophonology), Object-HSs display higher finger group complexity than Handling-HSs. Some adult homesigners, who have not acquired a signed or spoken language and instead use a self-generated gesture system, exhibit these two properties as well. This study illuminates the development over time of both phenomena for one child homesigner, "Julio," age 7;4 (years; months) to 12;8. We elicited descriptions of events with and without agents to determine whether morphophonology and morphosyntax can develop without linguistic input during childhood, and whether these structures develop together or independently. Within the time period studied: (1) Julio used handshape type differently in his responses to vignettes with and without an agent; however, he did not exhibit the same pattern that was found previously in signers, adult homesigners, or gesturers: while he was highly likely to use a Handling-HS for events with an agent (82%), he was less likely to use an Object-HS for non-agentive events (49%); i.e., his productions were heavily biased toward Handling-HSs; (2) Julio exhibited higher finger group complexity in Object- than in Handling-HSs, as in the sign language and adult homesigner groups previously studied; and (3) these two dimensions of language developed independently, with phonological structure showing a sign language-like pattern at an earlier age than morphosyntactic structure. We conclude that iconicity alone is not sufficient to explain the development of linguistic structure in homesign systems. Linguistic input is not required for some aspects of phonological structure to emerge in childhood, and while linguistic input is not required for morphology either, it takes time to emerge in homesign.

iconicity
homesign
sign language
morphology
gesture
phonology
grammaticalization
language emergence


Modulation of Cellular Biochemistry, Epigenetics and Metabolomics by Ketone Bodies. Implications of the Ketogenic Diet in the Physiology of the Organism and Pathological States.

Abstract: Ketone bodies (KBs), comprising β-hydroxybutyrate, acetoacetate and acetone, are a set of fuel molecules serving as an alternative energy source to glucose. KBs are mainly produced by the liver from fatty acids during periods of fasting, and prolonged or intense physical activity. In diabetes, mainly type-1, ketoacidosis is the pathological response to glucose malabsorption. Endogenous production of ketone bodies is promoted by consumption of a ketogenic diet (KD), a diet virtually devoid of carbohydrates. Despite its recently widespread use, the systemic impact of KD is only partially understood, and ranges from physiologically beneficial outcomes in particular circumstances to potentially harmful effects. Here, we firstly review ketone body metabolism and molecular signaling, to then link the understanding of ketone bodies' biochemistry to controversies regarding their putative or proven medical benefits. We overview the physiological consequences of ketone bodies' consumption, focusing on (i) KB-induced histone post-translational modifications, particularly β-hydroxybutyrylation and acetylation, which appears to be the core epigenetic mechanisms of activity of β-hydroxybutyrate to modulate inflammation; (ii) inflammatory responses to a KD; (iii) proven benefits of the KD in the context of neuronal disease and cancer; and (iv) consequences of the KD's application on cardiovascular health and on physical performance.

Dąbek|Arkadiusz|A| Wojtala|Martyna|M| Pirola|Luciano|L| Balcerczyk|Aneta|A|

epigenetics
ketone bodies
ketogenic diet
β-hydroxybutyrate
inflammatory response
cancer


Prenatal Exposure to Aluminum and Status of Selected Essential Trace Elements in Rural South African Women at Delivery.

Abstract: This study sought to evaluate the in utero exposure to aluminum and status of selected trace elements in South African women at delivery since aluminum is known to be toxic in all developmental stages even at low concentrations. Serum aluminum was negatively correlated with aluminum in urine, both uncorrected and corrected for creatinine, which suggests the retention of aluminum in body stores. Serum copper and zinc levels were found to be high in this study population. Serum copper levels were negatively correlated with aluminum in serum (β = -0.095; p = 0.05). There was a marginal negative correlation between aluminum levels in serum and manganese levels in whole blood (β = -0.087; p = 0.08). Copper levels in maternal serum were negatively correlated with birth weight and the length of neonates. There were a number of positive correlations between maternal characteristics and birth outcomes. Mothers who consumed root vegetables frequently appeared to be protected from aluminum retention and increased body burden since their serum aluminum levels were found to be significantly lower. The findings of the current study can be used as a baseline for further research on aluminum exposure and its associated interactions and outcomes in vulnerable populations.

Röllin|Halina B|HB|0000-0001-5247-6519
Nogueira|Claudina|C|
Olutola|Bukola|B|
Channa|Kalavati|K|
Odland|Jon Ø|JØ|0000-0002-2756-0732

aluminum
essential trace elements
in utero exposure
birth outcomes
maternal serum and urine


How anthropogenic changes may affect soil-borne parasite diversity? Plant-parasitic nematode communities associated with olive trees in Morocco as a case study.

Abstract:
BACKGROUND
Plant-parasitic nematodes (PPN) are major crop pests. On olive (Olea europaea), they significantly contribute to economic losses in the top-ten olive producing countries in the world especially in nurseries and under cropping intensification. The diversity and the structure of PPN communities respond to environmental and anthropogenic forces. The olive tree is a good host plant model to understand the impact of such forces on PPN diversity since it grows according to different modalities (wild, feral and cultivated olives). A wide soil survey was conducted in several olive-growing regions in Morocco. The taxonomical and the functional diversity as well as the structures of PPN communities were described and then compared between non-cultivated (wild and feral forms) and cultivated (traditional and high-density olive cultivation) olives.

RESULTS
A high diversity of PPN with the detection of 117 species and 47 genera was revealed. Some taxa were recorded for the first time on olive trees worldwide and new species were also identified. Anthropogenic factors (wild vs cultivated conditions) strongly impacted the PPN diversity and the functional composition of communities because the species richness, the local diversity and the evenness of communities significantly decreased and the abundance of nematodes significantly increased in high-density conditions. Furthermore, these conditions exhibited many more obligate and colonizer PPN and less persister PPN compared to non-cultivated conditions. Taxonomical structures of communities were also impacted: genera such as Xiphinema spp. and Heterodera spp. were dominant in wild olive, whereas harmful taxa such as Meloidogyne spp. were especially enhanced in high-density orchards.

CONCLUSIONS
Olive anthropogenic practices reduce the PPN diversity in communities and lead to changes of the community structures with the development of some damaging nematodes. The study underlined the PPN diversity as a relevant indicator to assess community pathogenicity. That could be taken into account in order to design control strategies based on community rearrangements and interactions between species instead of reducing the most pathogenic species.

Ali|Nadine|N|
Tavoillot|Johannes|J|
Besnard|Guillaume|G|
Khadari|Bouchaib|B|
Dmowska|Ewa|E|
Winiszewska|Grażyna|G|
Fossati-Gaschignard|Odile|O|
Ater|Mohammed|M|
Aït Hamza|Mohamed|M|
El Mousadik|Abdelhamid|A|
El Oualkadi|Aïcha|A|
Moukhli|Abdelmajid|A|
Essalouh|Laila|L|
El Bakkali|Ahmed|A|
Chapuis|Elodie|E|
Mateille|Thierry|T|

Plant-parasitic nematodes
Communities
Olive
Morocco
Functional diversity
Taxonomical structures
Anthropisation


Efficacy and safety of tamsulosin hydrochloride 0.2 mg and combination of tamsulosin hydrochloride 0.2 mg plus solifenacin succinate 5 mg after transurethral resection of the prostate: a prospective, randomized controlled trial.

Abstract:
OBJECTIVE
The objective of this study was to evaluate the safety and efficacy of tamsulosin hydrochloride 0.2 mg (TAM) and its combination with solifenacin succinate 5 mg (SOL) after transurethral resection of the prostate (TURP).

PATIENTS AND METHODS
The patients were randomized into three groups: TURP (group 1), TURP plus TAM (group 2), and TURP plus TAM + SOL (group 3). Patients in group 2 and group 3 received medication for 4 weeks. The primary efficacy end points were the mean change in total International Prostate Symptom Score (IPSS) and IPSS subscores. The secondary end points included quality-of-life score, Overactive Bladder Symptom Score, and short-form voiding and storage score of International Continence Society.

RESULTS
In total, 37 men (31.8%) in group 1, 37 men (31.8%) in group 2, and 42 men (36.2%) in group 3 completed the study. In total IPSS, no significant improvement was seen from baseline to the end of treatment in groups 2 and 3 compared with group 1. However, in group 2, the decrement in the IPSS storage score was smaller than group 1 (P=0.02), and in group 3, the decrement in the IPSS voiding score was smaller than group 1 (P=0.05). In groups 2 and 3 compared with group 1, improvements in the quality of life score, total score of Overactive Bladder Symptom Score, and short-form voiding score and storage score of International Continence Society were not statistically significant.

CONCLUSION
Treatment with TAM and combination of TAM and SOL did not have significant additional benefits for lower urinary tract symptoms during the early recovery period after TURP.

Shin|Yu Seob|YS|
Zhang|Li Tao|LT|
You|Jae Hyung|JH|
Choi|In Sung|IS|
Zhao|Chen|C|
Park|Jong Kwan|JK|

D020728:Transurethral Resection of Prostate

tamsulosin
combination
solifenacin
transurethral resection of the prostate
benign prostate hyperplasia


Predictive Maintenance of Boiler Feed Water Pumps Using SCADA Data.

Abstract: IoT enabled predictive maintenance allows companies in the energy sector to identify potential problems in the production devices far before the failure occurs. In this paper, we propose a method for early detection of faults in boiler feed pumps using existing measurements currently captured by control devices. In the experimental part, we work on real measurement data and events from a coal fired power plant. The main research objective is to implement a model that detects deviations from the normal operation state based on regression and to check which events or failures can be detected by it. The presented technique allows the creation of a predictive system working on the basis of the available data with a minimal requirement of expert knowledge, in particular the knowledge related to the categorization of failures and the exact time of their occurrence, which is sometimes difficult to identify. The paper shows that with modern technologies, such as the Internet of Things, big data, and cloud computing, it is possible to integrate automation systems, designed in the past only to control the production process, with IT systems that make all processes more efficient through the use of advanced analytic tools.

predictive maintenance
SCADA
boiler feed pump
Internet of Things
anomaly detection


A modeling informed quantitative approach to salvage clinical trials interrupted due to COVID-19.

Abstract: Many ongoing Alzheimer's disease central nervous system clinical trials are being disrupted and halted due to the COVID-19 pandemic. They are often of a long duration' are very complex; and involve many stakeholders, not only the scientists and regulators but also the patients and their family members. It is mandatory for us as a community to explore all possibilities to avoid losing all the knowledge we have gained from these ongoing trials. Some of these trials will need to completely restart, but a substantial number can restart after a hiatus with the proper protocol amendments. To salvage the information gathered so far, we need out-of-the-box thinking for addressing these missingness problems and to combine information from the completers with those subjects undergoing complex protocols deviations and amendments after restart in a rational, scientific way. Physiology-based pharmacokinetic (PBPK) modeling has been a cornerstone of model-informed drug development with regard to drug exposure at the site of action, taking into account individual patient characteristics. Quantitative systems pharmacology (QSP), based on biology-informed and mechanistic modeling of the interaction between a drug and neuronal circuits, is an emerging technology to simulate the pharmacodynamic effects of a drug in combination with patient-specific comedications, genotypes, and disease states on functional clinical scales. We propose to combine these two approaches into the concept of computer modeling-based virtual twin patients as a possible solution to harmonize the readouts from these complex clinical datasets in a biologically and therapeutically relevant way.


KNN-MDR: a learning approach for improving interactions mapping performances in genome wide association studies.

Abstract:
BACKGROUND
Finding epistatic interactions in large association studies like genome-wide association studies (GWAS) with the nowadays-available large volume of genomic data is a challenging and largely unsolved issue. Few previous studies could handle genome-wide data due to the intractable difficulties met in searching a combinatorial explosive search space and statistically evaluating epistatic interactions given a limited number of samples. Our work is a contribution to this field. We propose a novel approach combining K-Nearest Neighbors (KNN) and Multi Dimensional Reduction (MDR) methods for detecting gene-gene interactions as a possible alternative to existing algorithms, e especially in situations where the number of involved determinants is high. After describing the approach, a comparison of our method (KNN-MDR) to a set of the other most performing methods (i.e., MDR, BOOST, BHIT, MegaSNPHunter and AntEpiSeeker) is carried on to detect interactions using simulated data as well as real genome-wide data.

RESULTS
Experimental results on both simulated data and real genome-wide data show that KNN-MDR has interesting properties in terms of accuracy and power, and that, in many cases, it significantly outperforms its recent competitors.

CONCLUSIONS
The presented methodology (KNN-MDR) is valuable in the context of loci and interactions mapping and can be seen as an interesting addition to the arsenal used in complex traits analyses.

D004843:Epistasis, Genetic
D055106:Genome-Wide Association Study
D006801:Humans
D020641:Polymorphism, Single Nucleotide

Genome-wide association study
K-nearest neighbors
Multi dimensional reduction
Gene-gene interaction
Epistasis
Single nucleotide polymorphism


Synthesis of 6-chloro-2-Aryl-1H-imidazo[4,5-b]pyridine derivatives: Antidiabetic, antioxidant, β-glucuronidase inhibiton and their molecular docking studies.

Abstract: 6-Chloro-2-Aryl-1H-imidazo[4,5-b]pyridine derivatives 1-26 were synthesized and characterized by various spectroscopic techniques. All these derivatives were evaluated for their antiglycation, antioxidant and β-glucuronidase potential followed their docking studies. In antiglycation assay, compound 2 (IC50=240.10±2.50μM) and 4 (IC50=240.30±2.90μM) was found to be most active compound of this series, while compounds 3 (IC50=260.10±2.50μM), 6 (IC50=290.60±3.60μM), 13 (IC50=288.20±3.00μM) and 26 (IC50=292.10±3.20μM) also showed better activities than the standard rutin (IC50=294.50±1.50μM). In antioxidant assay, compound 1 (IC50=69.45±0.25μM), 2 (IC50=58.10±2.50μM), 3 (IC50=74.25±1.10μM), and 4 (IC50=72.50±3.30μM) showed good activities. In β-glucuronidase activity, compounds 3 (IC50=29.25±0.50μM), compound 1 (IC50=30.10±0.60μM) and compound 4 (IC50=46.10±1.10μM) showed a significant activity as compared to than standard D-Saccharic acid 1,4-lactonec (IC50=48.50±1.25μM) and their interaction with the enzyme was confirm by docking studies.

Taha|Muhammad|M|
Ismail|Nor Hadiani|NH|
Imran|Syahrul|S|
Rashwan|Hesham|H|
Jamil|Waqas|W|
Ali|Sajjad|S|
Kashif|Syed Muhammad|SM|
Rahim|Fazal|F|
Salar|Uzma|U|
Khan|Khalid Mohammed|KM|


The Diabetic Retinopathy Clinical Research Network (DRCR.net) and Its Contributions to the Treatment of Diabetic Retinopathy.

Abstract: Over the past two decades, the Diabetic Retinopathy Clinical Research Network (now known as the DRCR Retina Network) has contributed to multiple and substantial advances in the clinical care of diabetic eye disease. Network studies helped establish anti-vascular endothelial growth factor (VEGF) agents as an effective alternative to panretinal photocoagulation for eyes with proliferative diabetic retinopathy (PDR) and as first-line therapy for eyes with visual impairment for diabetic macular edema (DME), defined treatment algorithms for the use of intravitreal medications in these conditions, and provided critical data to understand how to better evaluate the diabetic eye using optical coherence tomography and other imaging modalities. Ongoing DRCR.net studies will address whether anti-VEGF therapy is effective at preventing vision-threatening complications in eyes with severe non-proliferative diabetic retinopathy, if photobiomodulation has a beneficial effect in eyes with DME, and whether initiation of DME treatment with bevacizumab and rescue with aflibercept can provide visual outcomes as good as those achieved with aflibercept alone. Future plans for the Network also include the expansion into non-diabetic eye disease in areas such as age-related macular degeneration.

D020533:Angiogenesis Inhibitors
D002985:Clinical Protocols
D003930:Diabetic Retinopathy
D006801:Humans
D058449:Intravitreal Injections
D017075:Laser Coagulation
D012160:Retina
D041623:Tomography, Optical Coherence
D014792:Visual Acuity


Bone Status in Patients with Phenylketonuria: A Systematic Review.

Abstract: Phenylketonuria (PKU) is the most common inborn error of amino acid metabolism. Although dietary and, in some cases, pharmacological treatment has been successful in preventing intellectual disability in PKU patients who are treated early, suboptimal outcomes have been reported, including bone mineral disease. In this systematic review, we summarize the available evidence on bone health in PKU patients, including data on bone mineral density (BMD) and bone turnover marker data. Data from cohort and cross-sectional studies of children and adults (up to 40 years of age) were obtained by searching the MEDLINE and SCOPUS databases following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. For each selected study, quality assessment was performed applying the Risk Of Bias In Non-randomized Studies of Interventions (ROBINS I) tool. We found that mean BMD was lower in PKU patients than in reference groups, but was within the normal range in most patients when expressed as Z-score values. Furthermore, data revealed a trend towards an imbalance between bone formation and bone resorption, favoring bone removal. Data on serum levels of minerals and hormones involved in bone metabolism were very heterogeneous, and the analyses were inconclusive. Clinical trials that include the analysis of fracture rates, especially in older patients, are needed to gather more evidence on the clinical implications of lower BMD in PKU patients.

bone
bone mineral density
bone turnover markers
fractures
hyperphenylalaninemia
osteopenia


Novel dental resin composites containing LiAl-F layered double hydroxide (LDH) filler: Fluoride release/recharge, mechanical properties, color change, and cytotoxicity.

Abstract:
OBJECTIVES
A novel LiAl-F layered double hydroxide (LDH) with a beneficial anions-exchangeable feature was developed for use as a fluoride reservoir. This study aims to investigate the fluoride release/ recharge capability of an LDH-contained dental resin composite and their effects on physical and biological properties.

METHODS
3% and 5% of LDH (R3, R5), wet-milled micro-scale LDH (R3W, R5W), or dry-milled nano-scale LDH (R3D, R5D) were added to a micro-hybrid flowable resin composite (RX). A commercial dental compomer (CC) was selected for comparison. An ion selective electrode recorded the daily fluoride release for 90 days (Daily fluoride recharging from 30th to 60th day). The flexural tests were performed according to ISO 4049. Also, the surface microhardness, color changes (L*, a*, b*), Al3+/Li+ ion leaching, and cytotoxicity were evaluated.

RESULTS
The LDH-contained resin composites show significantly increased fluoride release and recharge capability. Among them, R3 and R5 have more stable and long-term fluoride release than R3W, R5W, R3D, R5D, and CC. The LDH filler did not induce cytotoxicity, although few amounts of Al3+/Li+ ions were detected on the first day. The LDH filler did not alter the flexural strength of RX, and the microhardness (R3, R5, R3D, and R5D) was significantly higher than the CC group (P < 0.01). The LDH filler slightly increased the color of the composite in red (a* values) and yellow (b* values).

SIGNIFICANCE
The LiAl-F LDH can be a fluoride reservoir filler for dental resin composites that increases the anti-caries ability.

Wei Su|Liang|L|
Lin|Dan Jae|DJ|
Yen Uan|Jun|J|

Resin composites
Layered double hydroxide
Fluoride release
Recharge capability
Restorations


Outcome and prognostic factors after lung transplantation for bronchiectasis other than cystic fibrosis.

Abstract:
BACKGROUND
While lung transplant (LTX) can be an effective therapy to provide the survival benefit in selected populations, post-transplant outcome in LTX recipients with bronchiectasis other than cystic fibrosis (CF) has been less studied. Pseudomonas aeruginosa, often associated with exacerbations in bronchiectasis, is the most common micro-organism isolated from LTX recipients. We aimed to see the outcomes of patients with bronchiectasis other than CF after LTX and seek the risk factors associated with pre- and post-transplant Pseudomonas status.

METHODS
Patients who underwent LTX at Tohoku University Hospital between January 2000 and December 2020 were consecutively included into the retrospective cohort study. Pre- and post-transplant prevalence of Pseudomonas colonization between bronchiectasis and other diseases was reviewed. Post-transplant outcomes (mortality and the development of chronic lung allograft dysfunction (CLAD)) were assessed using a Cox proportional hazards and time-to-event outcomes were estimated using the Kaplan-Meier method.

RESULTS
LTX recipients with bronchiectasis experienced a high rate of pre- and post-transplant Pseudomonas colonization compared to other diseases with statistical significance (p < 0.001 and p < 0.001, respectively). Nevertheless, long-term survival in bronchiectasis was as great as non-bronchiectasis (Log-rank p = 0.522), and the bronchiectasis was not a trigger for death (HR 1.62, 95% CI 0.63-4.19). On the other hand, the chance of CLAD onset in bronchiectasis was comparable to non-bronchiectasis (Log-rank p = 0.221), and bronchiectasis was not a predictor of the development of CLAD (HR 1.88, 95% CI 0.65-5.40).

CONCLUSIONS
Despite high prevalence of pre- and post-transplant Pseudomonas colonization, the outcome in LTX recipients with bronchiectasis other than CF was comparable to those without bronchiectasis.

Lung transplant
Bronchiectasis
Pseudomonas aeruginosa
Chronic lung allograft dysfunction
Non-tuberculous mycobacteria
Sinusitis


Carboplatin therapeutic monitoring in preterm and full-term neonates.

Abstract:
INTRODUCTION
Administration of the most appropriate dose of chemotherapy to neonates is particularly challenging and frequently not standardised based on any scientific rationale. We report the clinical utility of carboplatin therapeutic drug monitoring in preterm and full-term neonates within the first month of life.

METHODS
Carboplatin therapeutic monitoring was performed to achieve target drug exposures area under the plasma concentration-time curve (AUC values) in nine preterm and full-term neonates diagnosed with retinoblastoma or neuroblastoma treated over an 8 year period. Carboplatin was administered over 3 days with therapeutic drug monitoring utilised to target cumulative AUC values of 5.2-7.8 mg/ml min.

RESULTS
AUC values achieved were within 15% of target values for the individual courses of treatment in all but one patient (12/13 courses of treatment), with dose modifications of up to 215% required to achieve target AUC values, based on initial mg/kg dosing schedules. Carboplatin clearance determined across three consecutive chemotherapy courses in two patients increased from 3.4 to 7.1 ml/min and from 7.2 to 16.5 ml/min, representing increases of 210-230% over several weeks of treatment. Complete remission was observed in 8/9 patients, with no renal toxicity reported and only one patient experiencing ototoxicity.

CONCLUSION
The study highlights the benefits of utilising therapeutic drug monitoring to achieve target carboplatin AUC values in preterm and full-term neonates treated within the first few weeks of life, particularly in view of marked increases in drug clearance observed over consecutive chemotherapy courses. In the absence of therapeutic drug monitoring, body-weight based dosing is recommended, with dosing guidance provided for both approaches to inform future treatment.

Veal|Gareth J|GJ|
Errington|Julie|J|
Hayden|James|J|
Hobin|David|D|
Murphy|Dermot|D|
Dommett|Rachel M|RM|
Tweddle|Deborah A|DA|
Jenkinson|Helen|H|
Picton|Susan|S|

D000970:Antineoplastic Agents
D019540:Area Under Curve
D016190:Carboplatin
D004334:Drug Administration Schedule
D016903:Drug Monitoring
D005865:Gestational Age
D006801:Humans
D007231:Infant, Newborn
D007234:Infant, Premature
D009447:Neuroblastoma
D011237:Predictive Value of Tests
D012074:Remission Induction
D012175:Retinoblastoma
D047929:Term Birth
D016896:Treatment Outcome

Carboplatin
Neonates
Chemotherapy
Therapeutic drug monitoring
Retinoblastoma
Neuroblastoma


Mebeverine-loaded electrospun nanofibers: physicochemical characterization and dissolution studies.

Abstract: Both fast dissolving and sustained release drug delivery systems (DDSs) comprising mebeverine hydrochloride (MB-HCl) embedded in either povidone (PVP) K60 or Eudragit(®) L 100-55 nanofibers have been prepared by electrospinning. The fibers are found to have cylindrical morphologies with smooth surfaces, except at high drug loadings that appear to induce surface roughness (PVP) or fragmentation (Eudragit). There is a general increase in fiber diameter with drug loading. Differential scanning calorimetry and X-ray diffraction demonstrate that the drug exists in an amorphous state in the fibers. Infrared spectroscopy data indicate that the drug has good compatibility with the polymer, whereas nuclear magnetic resonance spectroscopy and high-performance liquid chromatography analyses confirmed that the MB-HCl was not degraded during the spinning process. In vitro dissolution tests of the PVP fiber mats show them to dissolve within 10 s, an improved dissolution profile over the pure drug. The Eudragit fibers show pH-dependent drug release profiles, with only very limited release at pH 2.0 but sustained release over approximately 8 h at pH 6.8. The Eudragit nanofibers have the potential to be developed as oral DDSs for localized drug release in the intestinal tract, whereas the PVP materials may find the application as buccal delivery systems or suppositories.

Illangakoon|Upulitha Eranka|UE|
Nazir|Tahir|T|
Williams|Gareth R|GR|
Chatterton|Nicholas P|NP|

electrospun nanofibers
mebeverine hydrochloride
povidone
Eudragit
buccal delivery
nanotechnology
oral drug delivery
site-specific delivery
solid dosage form
solid solutions


Genomewide transcriptional signatures of migratory flight activity in a globally invasive insect pest.

Abstract: Migration is a key life history strategy for many animals and requires a suite of behavioural, morphological and physiological adaptations which together form the 'migratory syndrome'. Genetic variation has been demonstrated for many traits that make up this syndrome, but the underlying genes involved remain elusive. Recent studies investigating migration-associated genes have focussed on sampling migratory and nonmigratory populations from different geographic locations but have seldom explored phenotypic variation in a migratory trait. Here, we use a novel combination of tethered flight and next-generation sequencing to determine transcriptomic differences associated with flight activity in a globally invasive moth pest, the cotton bollworm Helicoverpa armigera. By developing a state-of-the-art phenotyping platform, we show that field-collected H. armigera display continuous variation in flight performance with individuals capable of flying up to 40 km during a single night. Comparative transcriptomics of flight phenotypes drove a gene expression analysis to reveal a suite of expressed candidate genes which are clearly related to physiological adaptations required for long-distance flight. These include genes important to the mobilization of lipids as flight fuel, the development of flight muscle structure and the regulation of hormones that influence migratory physiology. We conclude that the ability to express this complex set of pathways underlines the remarkable flexibility of facultative insect migrants to respond to deteriorating conditions in the form of migratory flight and, more broadly, the results provide novel insights into the fundamental transcriptional changes required for migration in insects and other taxa.

Jones|Christopher M|CM|
Papanicolaou|Alexie|A|
Mironidis|George K|GK|
Vontas|John|J|
Yang|Yihua|Y|
Lim|Ka S|KS|
Oakeshott|John G|JG|
Bass|Chris|C|
Chapman|Jason W|JW|

tethered flight
transcriptomics
insect migration
migratory genomics


Masculinities, practices and meanings: A critical analysis of recent literature on the use of performance- and image-enhancing drugs among men.

Abstract: The use of performance- and image-enhancing drugs (PIEDs) has been a topic of considerable research interest since the 1980s, with the vast majority of PIED consumers being men. In this article, our departure point is a 2005 article by Helen Keane, in which she critically analyses 'the discursive constitution of male steroid users' as psychologically disordered subjects. We extend Keane's insightful feminist analysis by examining the constitution of masculinity in post-2005 social science research on PIEDs. We ask (1) to what extent do the discursive trends identified by Keane persist in the more recent literature on PIED use among men? (2) how have her insights been taken up in the post-2005 literature, and (3) to what extent does this work attend to the specificity and varied meanings of steroid practices? We argue that men who use PIEDs continue to be pathologised as insecure, inadequate and vulnerable, and marked by 'obsession', 'compensatory behaviours' and crisis. In some of the analysed texts, the male steroid user becomes doubly disordered as both insecure in his masculine body and at risk of drug dependence. Of the articles that engage with Keane's work, only two recognise the value of her insights. The others misinterpret or apply Keane's argument in inconsistent or incoherent ways. Finally, in some of the post-2005 texts, we begin to see attention to the wide variety of practices and meanings encompassed by the term 'PIED use' although much remains to be learned.

masculinities practices meanings men PIEDs


Designing an Authorization System Based on Patient Privacy Preferences in Japan.

Abstract: High accessibility of Electronic Health Record systems can increase usability but creates simultaneously patients' anxieties about privacy issues. In order to reduce the privacy concerns, we focused on control and awareness, and designed an approach that can provide availability of patient's clinical data to doctors in two scenarios; (S1) direct control by the patient when they are conscious, (S2) control by a trusted representative when the patient is unconscious. In this paper, we show further analysis in a survey (n = 310, age range: 19-91) done to test the acceptability of our concept of a using a trusted representative and to further understand the concerns of Japanese citizens to improve our system design. These results in S1 suggest that patients concerned about control have a stronger inclination to also choose full awareness. We found also that patients tended to choose the same level of awareness for the representative as they did for themselves in S2. In addition, patients who chose awareness in S1 tended to choose the same for their representative in S2 and themselves after recovery from unconsciousness. We also discuss the significant differences found between the age-groups 20-39 and 60-79. We conclude that the system design of privacy aware EHR systems must be improved to consider patients who want to preserve their choice of control in the event they become unconscious but do not want to use a representative to maintain control.

Morris|Kensuke|K| Yamamoto|Goshiro|G| Hiragi|Shusuke|S| Ohtera|Shosuke|S| Sakai|Michi|M| Sugiyama|Osamu|O| Okamoto|Kazuya|K| Nambu|Masayuki|M| Kuroda|Tomohiro|T|

Privacy Concerns
EHRs
Access Control
Patient-Centred Authorization


Involvement of Atm and Trp53 in neural cell loss due to Terf2 inactivation during mouse brain development.

Abstract: Maintenance of genomic integrity is one of the critical features for proper neurodevelopment and inhibition of neurological diseases. The signals from both ATM and ATR to TP53 are well-known mechanisms to remove neural cells with DNA damage during neurogenesis. Here we examined the involvement of Atm and Atr in genomic instability due to Terf2 inactivation during mouse brain development. Selective inactivation of Terf2 in neural progenitors induced apoptosis, resulting in a complete loss of the brain structure. This neural loss was rescued partially in both Atm and Trp53 deficiency, but not in an Atr-deficient background in the mouse. Atm inactivation resulted in incomplete brain structures, whereas p53 deficiency led to the formation of multinucleated giant neural cells and the disruption of the brain structure. These giant neural cells disappeared in Lig4 deficiency. These data demonstrate ATM and TP53 are important for the maintenance of telomere homeostasis and the surveillance of telomere dysfunction during neurogenesis.

D064007:Ataxia Telangiectasia Mutated Proteins

D035341:Telomeric Repeat Binding Protein 2

Atm
Brain development
DNA damage
Apoptosis


Recombinant Factor VIIa in Post-partum Hemorrhage: A New Weapon in Obstetrician's Armamentarium.

Abstract: Post-partum hemorrhage (PPH) is a life-threatening obstetric complication and the leading cause of maternal death. The usual manner for its management includes, first, noninvasive and nonsurgical methods, and, then invasive and surgical methods. However, mortality and morbidity related to PPH still remains unacceptably high, contributing to hysterectomy in at least 50% of cases. Early, effective, and preferably noninvasive treatments that can reduce maternal mortality and morbidity due to this entity are therefore essential. One of the most spectacular advancements in the control of PPH has been the use of recombinant activated factor (rFVIIa), both as initial and a life- and uterus-saving therapy. rFVIIa also reduces costs of therapy and use of blood components in massive PPH. In cases of intractable bleeding with no other obvious indications for hysterectomy, administration of rFVIIa should be considered before surgery. A MEDLINE search was done to review relevant articles in English literature on use of rFVIIa in PPH. Data were constructed and issues were reviewed from there. Our experience in a series of three cases of PPH, two of atonic and one of traumatic, successfully managed using rFVIIa is also shared.

Novoseven
Postpartum hemorrhage
Recombinant factor-VIIa


Rate of Return to Sport and Functional Outcomes After Bilateral Hip Arthroscopy in High-Level Athletes.

Abstract:
BACKGROUND
Bilateral hip symptoms are common in athletes, and athletes may require treatment with bilateral hip arthroscopy. Return-to-sport (RTS) rates in competitive athletes after unilateral procedures have been reported at 74% to 93%; however, RTS rates after bilateral hip arthroscopy are still unknown.

PURPOSE/HYPOTHESIS
The purpose was to determine rate of RTS in competitive athletes undergoing bilateral hip arthroscopy and report minimum 1-year patient-reported outcomes (PROs) for this cohort. We hypothesized that after bilateral hip arthroscopy, the RTS rate would be similar to the square of the probability of returning after unilateral hip arthroscopy.

STUDY DESIGN
Case series; Level of evidence, 4.

METHODS
Data were prospectively collected on patients undergoing hip arthroscopy at our institution from November 2011 to July 2018. Patients were included if they underwent bilateral hip arthroscopy and were a high school, collegiate, or professional athlete before their first surgery. A patient's RTS was defined as return to competitive participation in one's sport at a level the same as or higher than the preoperative level. Additionally, minimum 1-year PROs, including modified Harris Hip Score (mHHS), nonarthritic hip score, and Hip Outcome Score-Sports Specific Subscale (HOS-SSS), as well as complication rates and future surgery were compared for all patients. Rates of reaching the minimal clinically importance difference (MCID) and patient acceptable symptomatic state (PASS) for the mHHS (8 and 74, respectively) and HOS-SSS (6 and 75, respectively) were also recorded.

RESULTS
A total of 87 patients met inclusion criteria, for which follow-up was available for 82 (94.3%). At latest follow-up, 100% of professional athletes had returned to their sport, while 53.7% of the entire cohort returned to their sport, with 75.8% of male patients returning versus 38.8% of female patients (P < .001). Of patients returning, 56% did so at the same ability or higher. The most common reason for not returning was graduation or lifestyle change (47.4%). Patients returning to sport had significantly higher PROs at latest follow-up relative to those who did not return, including mHHS (93.7 vs 87.5), nonarthritic hip score (94.4 vs 88.2), and HOS-SSS (90.9 vs 78.2) (P < .05). Rates of achieving the PASS and MCID for the mHHS were not significantly different. However, for the HOS-SSS, patients who returned had significantly higher rates of achieving the MCID and PASS thresholds.

CONCLUSION
The rate of RTS among competitive athletes after bilateral hip arthroscopy was similar to the square of published RTS rates after unilateral hip arthroscopy. Both those who returned to play and those who did not showed significant improvement in PROs after surgery. However, those who returned to sports achieved significantly higher scores in all outcome measures. Additionally, patients returning to sports showed a significantly higher rate of attaining the MCID and PASS scores for the HOS-SSS.

Rosinsky|Philip J|PJ|
Kyin|Cynthia|C|
Lall|Ajay C|AC|
Shapira|Jacob|J|
Maldonado|David R|DR|
Domb|Benjamin G|BG|

D000071066:Patient Reported Outcome Measures

return to sports
hip arthroscopy
bilateral surgery
femoroacetabular impingement


Eugenia punicifolia (Kunth) DC. as an adjuvant treatment for type-2 diabetes mellitus: a non-controlled, pilot study.

Abstract: Type-2 diabetes mellitus (DM) is a highly prevalent disease with significant morbidity and mortality around the world. However, there is no universally effective treatment, because response to different treatment regimens can vary widely among patients. In this study, we aimed to investigate whether the use of the powdered dried leaves of Eugenia punicifolia (Kunth) DC. (Myrtaceae) is effective as an adjuvant to the treatment of patients with type-2 DM. Fifteen patients were enrolled in a pilot, non-controlled study, and received E. punicifolia for 3 months. After treatment, we observed a significant decrease in glycosylated hemoglobin, basal insulin, thyroid-stimulating hormone, C-reactive protein, and both systolic and diastolic blood pressure. There were no changes in fasting and postprandial glycemia. The compounds myricetin-3-O-rhamnoside, quercetin-3-O-galactoside, quercetin-3-O-xyloside, quercetin-3-O-rhamnoside, kaempferol-3-O-rhamnoside, phytol, gallic acid, and trans-caryophyllene present in the powdered dried leaves of E. punicifolia may be responsible for the therapeutic effect. In conclusion, the powdered leaves of E. punicifolia are promising as an adjuvant in the treatment of type-2 DM and deserve further investigation.

Sales|Débora Simone|DS|
Carmona|Fabio|F|
de Azevedo|Bruna Cestari|BC|
Taleb-Contini|Silvia Helena|SH|
Bartolomeu|Ana Carolina Duó|AC|
Honorato|Fernando B|FB|
Martinez|Edson Z|EZ|
Pereira|Ana Maria Soares|AM|

myrtaceae
herbal medicine
hypoglycemic agents
phytotherapeutics


Translation and validation of the Chinese version of the short Warwick-Edinburgh Mental Well-being Scale for patients with mental illness in Hong Kong.

Abstract:
OBJECTIVE
Quality of life outcomes are useful in the assessment of mental and social wellbeing and for informed health care decision-making, especially in the choice of interventions in psychiatric rehabilitation. In its original form, the Warwick-Edinburgh Mental Well-being Scale (WEMWBS) is a proven reliable and valid tool for assessing quality of life in normal adults, but not in adults from Asian countries. A shortened 7-item version of WEMWBS (SWEMWBS) with good internal construct validity was used for this study. The present study describes the translation of WEMWBS from English to Chinese and its validation in a sample of Chinese-speaking patient population.

METHODS
Participants included patients admitted to the inpatient units, and those attending the day hospital and outpatient units of the Kowloon Hospital (n = 126). Translation was performed using the multiple forward and backward translation protocol. Patients also completed the 5-item World Health Organization Well-being Index (WHO5) questionnaire. A case therapist completed the Brief Psychiatric Rating Scale within 2 days. A total of 20 patients were selected for test-retest measurements performed after 2 weeks.

RESULTS
The sample displayed a normal distribution of the Chinese version of SWEMWBS (C-SWEMWBS) scores (mean ± standard deviation, 23.16 ± 5.39; skewness, -0.068; kurtosis, -0.355). Internal reliability coefficient (Cronbach's alpha) for C-SWEMWBS was 0.89 which was consistent with that of English version. The corrected item-total correlation was high with Spearman's rank correlation coefficients ranging from 0.57 (item 6) to 0.75 (item 5). Good test-retest reliability was observed (r = 0.677; p = 0.001). Principal components factor analysis identified a single component (eigenvalues, 4.28; 61.1% variance), similar to the English version. Scores of C-SWEMWBS were positively correlated with the scores of WHO5 (r = 0.49; p < 0.001), suggesting good concurrent validity. Few item scores including 'feeling useful', 'dealing with problems well', 'able to make decisions', and the total score were significantly correlated with diagnostic groups (p < 0.05). Education and diagnosis of mental illness were valid predictors for C-SWEMWBS (F = 5.41; p = 0.01). There were no effects due to age and gender.

CONCLUSION
The C-SWEMWBS showed high levels of internal consistency and reliability against accepted criteria. It is short, acceptable, and culturally meaningful to clients with mental illness. Further large-scale studies in normal subjects and varied patient groups are recommended to generalise the findings.

Ng|S S W|SS|
Lo|A W Y|AW|
Leung|T K S|TK|
Chan|F S M|FS|
Wong|A T Y|AT|
Lam|R W T|RW|
Tsang|D K Y|DK|

D011569:Psychiatric Status Rating Scales

Quality of life
Mental disorders
Mental health


Behavioural responses of freshwater planarians after short-term exposure to the insecticide chlorantraniliprole.

Abstract: Recent advances in video tracking technologies provide the tools for a sensitive and reproducible analysis of invertebrate activity under stressful conditions nurturing the field of behavioural ecotoxicology. This study aimed to evaluate behavioural responses of the freshwater planarian Dugesia subtentaculata exposed to a model compound, chlorantraniliprole (CAP). This compound is an anthranilic diamide insecticide and due to its neurotoxic action can, at low concentrations, impair behaviour of exposed organisms. Behavioural endpoints measured included feeding and locomotor activities. Feeding responses were based on planarian predatory behaviour using Chironomus riparius larvae as prey. Locomotion was measured by the traditional planarian locomotor velocity (pLMV) assay and additionally using an automated video tracking system using a Zebrabox(®) (Viewpoint, France) device. While feeding and pLMV were significantly impaired at 131.7μg/L CAP, the video tracking system showed that total distance covered by planarians was significantly reduced at concentrations as low as 26.2μg/L CAP. Our results show that more advanced automated video recording systems can be used in the development of sensitive bioassays allowing a reliable, time- and cost-effective quantification of behaviour in aquatic invertebrates. Due to their ecological relevance, behavioural responses should not be disregarded in risk assessment strategies and we advocate the suitability of planarians as suitable organisms for behavioural ecotoxicological studies.

Rodrigues|Andreia C M|ACM|
Henriques|Jorge F|JF|
Domingues|Inês|I|
Golovko|Oksana|O|
Žlábek|Vladimír|V|
Barata|Carlos|C|
Soares|Amadeu M V M|AMVM|
Pestana|João L T|JLT|

Dugesia subtentaculata
Anthranilic diamides
Locomotion
Automated video tracking system
Feeding activity


Manual wheelchair's turning resistance: swivelling resistance parameters of front and rear wheels on different surfaces.

Abstract:
PURPOSE
Daily locomotion with a manual wheelchair includes curvilinear movements. However, little is known about the resisting forces in play during turning manoeuvres where the wheels are generally both rolling and swivelling. This study aimed at quantifying the swivelling resistance parameters of several wheels on different surfaces and to evaluate the effect of the curvature radius on these parameters.

MATERIALS AND METHODS
A specific test bench was designed allowing the swivelling resistance parameters of a wheel rolling while swivelling to be determined. Seven wheels (3 front and 4 rear wheels), three surfaces (plywood, linoleum and carpet), two loads (25 and 45 kg) and five curvature radii (from 0 to 0.4 m) were tested through a full factorial design experiment.

RESULTS
Results showed that the wheel type was the most influential factor on swivelling resistance parameters, followed by the surface and the curvature radius. The effect of the load on swivelling resistance parameters was found negligible when compared to the influence of other factors. A predictive model for swivelling resistance parameters of the different wheel/surface combinations was proposed, as a function of the curvature radius.

CONCLUSION
This study allowed the swivelling resistance parameters of different wheel/surface combinations to be quantified, as a function of the curvature radius of the wheel trajectory. Combined with data on rolling resistance, these data could now be used to assess energy losses during real life ambulation or to achieve more realistic behaviour in virtual rehabilitation environment.Implications for rehabilitationSwivelling resistances are increased by carpet surfaces compared to tile surfaces.Conversely to rolling resistance, castors wheels are less prone to swivelling resistance than rear wheelsThe swivelling resistance of a wheel rolling while swivelling is decreased compared to a pure swivelling movement.Combined with data on rolling resistance, these data on swivelling resistance would allow energy loss during daily life activity to be determined or as input data for the control of wheelchair simulator in virtual environment used for rehabilitation.

wheelchair turning Resistance swiveling surfaces


Functional associations between support use and forelimb shape in strepsirrhines and their relevance to inferring locomotor behavior in early primates.

Abstract: The evolution of primates is intimately linked to their initial invasion of an arboreal environment. However, moving and foraging in this milieu creates significant mechanical challenges related to the presence of substrates differing in their size and orientation. It is widely assumed that primates are behaviorally and anatomically adapted to movement on specific substrates, but few explicit tests of this relationship in an evolutionary context have been conducted. Without direct tests of form-function relationships in living primates it is impossible to reliably infer behavior in fossil taxa. In this study, we test a hypothesis of co-variation between forelimb morphology and the type of substrates used by strepsirrhines. If associations between anatomy and substrate use exist, these can then be applied to better understand limb anatomy of extinct primates. The co-variation between each forelimb long bone and the type of substrate used was studied in a phylogenetic context. Our results show that despite the presence of significant phylogenetic signal for each long bone of the forelimb, clear support use associations are present. A strong co-variation was found between the type of substrate used and the shape of the radius, with and without taking phylogeny into account, whereas co-variation was significant for the ulna only when taking phylogeny into account. Species that use a thin branch milieu show radii that are gracile and straight and have a distal articular shape that allows for a wide range of movements. In contrast, extant species that commonly use large supports show a relatively robust and curved radius with an increased surface area available for forearm and hand muscles in pronated posture. These results, especially for the radius, support the idea that strepsirrhine primates exhibit specific skeletal adaptations associated with the supports that they habitually move on. With these robust associations in hand it will be possible to explore the same variables in extinct early primates and primate relatives and thus improve the reliability of inferences concerning substrate use in early primates.

Fabre|Anne-Claire|AC|
Marigó|Judit|J|
Granatosky|Michael C|MC|
Schmitt|Daniel|D|

Radius
Ulna
Fine branches milieu
Humerus
Phylogenetic comparative analysis
Surface geometric morphometrics


Oncocytic poorly differentiated (insular) thyroid carcinoma mimicking metastatic adenocarcinoma. A case report and review of the literature.

Abstract: We report the cytohistologic and immunohistochemical findings of an oncocytic variant of poorly differentiated thyroid carcinoma in a 76-year old man with a prior history of prostatic adenocarcinoma. The man complained of a palpable nodule in the right thyroid lobe and cervical lymph node. Fine-needle aspiration (FNA) in both cases yielded solid clusters of cells/insulae, microfollicles, and isolated atypical cells. Considering the patient's past history, an initial diagnosis of metastasis from prostate adenocarcinoma was considered. However, immunohistochemical staining of liquid-based cytology specimens (Thin-Prep) showed diffuse positive results for TTF-1 and thyroglobulin. The patient underwent total thyroidectomy with bilateral neck dissection. Histologic and immunohistochemical evaluation showed a poorly differentiated oncocytic thyroid carcinoma with lymphovascular invasion and lymph node metastases. To our knowledge, this is the first description of the immunocytochemical evaluation of this rare variant of poorly differentiated thyroid carcinoma using FNA and liquid based cytology.

oncocytic
thyroid
cytology
immunohistochemistry
poorly differentiated carcinoma
prognosis


Tumor expression of calcium sensing receptor and colorectal cancer survival: Results from the nurses' health study and health professionals follow-up study.

Abstract: Although experimental evidence suggests calcium-sensing receptor (CASR) as a tumor-suppressor, the prognostic role of tumor CASR expression in colorectal carcinoma remains unclear. We hypothesized that higher tumor CASR expression might be associated with improved survival among colorectal cancer patients. We evaluated tumor expression levels of CASR by immunohistochemistry in 809 incident colorectal cancer patients within the Nurses' Health Study and the Health Professionals Follow-up Study. We used Cox proportional hazards regression models to estimate multivariable hazard ratio (HR) for the association of tumor CASR expression with colorectal cancer-specific and all-cause mortality. We adjusted for potential confounders including tumor biomarkers such as microsatellite instability, CpG island methylator phenotype, LINE-1 methylation level, expressions of PTGS2, VDR and CTNNB1 and mutations of KRAS, BRAF and PIK3CA. There were 240 colorectal cancer-specific deaths and 427 all-cause deaths. The median follow-up of censored patients was 10.8 years (interquartile range: 7.2, 15.1). Compared with patients with no or weak expression of CASR, the multivariable HRs for colorectal cancer-specific mortality were 0.80 [95% confidence interval (CI): 0.55-1.16] in patients with moderate CASR expression and 0.50 (95% CI: 0.32-0.79) in patients with intense CASR expression (p-trend = 0.003). The corresponding HRs for overall mortality were 0.85 (0.64-1.13) and 0.81 (0.58-1.12), respectively. Higher tumor CASR expression was associated with a lower risk of colorectal cancer-specific mortality. This finding needs further confirmation and if confirmed, may lead to better understanding of the role of CASR in colorectal cancer progression.

Momen-Heravi|Fatemeh|F|
Masugi|Yohei|Y|
Qian|Zhi Rong|ZR|
Nishihara|Reiko|R|
Liu|Li|L|
Smith-Warner|Stephanie A|SA|
Keum|NaNa|N|0000-0003-4671-0211
Zhang|Lanjing|L|
Tchrakian|Nairi|N|
Nowak|Jonathan A|JA|
Yang|Wanshui|W|
Ma|Yanan|Y|
Bowden|Michaela|M|
da Silva|Annacarolina|A|
Wang|Molin|M|
Fuchs|Charles S|CS|
Meyerhardt|Jeffrey A|JA|
Ng|Kimmie|K|
Wu|Kana|K|
Giovannucci|Edward|E|
Ogino|Shuji|S|
Zhang|Xuehong|X|0000-0002-8260-8508

D015972:Gene Expression Regulation, Neoplastic

D020084:Long Interspersed Nucleotide Elements

calcium
calcium sensing receptor
colorectal cancer
survival
progression
molecular pathological epidemiology
prospective cohorts


Exploring electron beam induced atomic assembly via reinforcement learning in a molecular dynamics environment.

Abstract: Atom-by-atom assembly of functional materials and devices is perceived as one of the ultimate targets of nanotechnology. Recently it has been shown that the beam of a scanning transmission electron microscope can be used for targeted manipulation of individual atoms. However, the process is highly dynamic in nature rendering control difficult. One possible solution is to instead train artificial agents to perform the atomic manipulation in an automated manner without need for human intervention. As a first step to realizing this goal, we explore how artificial agents can be trained for atomic manipulation in a simplified molecular dynamics environment of graphene with Si dopants, using reinforcement learning. We find that it is possible to engineer the reward function of the agent in such a way as to encourage formation of local clusters of dopants under different constraints. This study shows the potential for reinforcement learning in nanoscale fabrication, and crucially, that the dynamics learned by agents encode specific elements of important physics that can be learned.

reinforcement learning
molecular dynamics
graphene
dopants


Submerged membrane adsorption hybrid system using four adsorbents to remove nitrate from water.

Abstract: Nitrate contamination of ground and surface waters causes environmental pollution and human health problems in many parts of the world. This study tests the nitrate removal efficiencies of two ion exchange resins (Dowex 21K XLT and iron-modified Dowex 21K XLT (Dowex-Fe)) and two chemically modified bio-adsorbents (amine-grafted corn cob (AG corn cob) and amine-grafted coconut copra (AG coconut copra)) using a dynamic adsorption treatment system. A submerged membrane (microfiltration) adsorption hybrid system (SMAHS) was used for the continuous removal of nitrate with a minimal amount of adsorbents. The efficiency of membrane filtration flux and replacement rate of adsorbent were studied to determine suitable operating conditions to maintain the effluent nitrate concentration below the WHO drinking standard limit of 11.3 mg N/L. The volume of water treated and the amount of nitrate adsorbed per gramme of adsorbent for all four flux tested were in the order Dowex-Fe > Dowex > AG coconut copra > AG corn cob. The volumes of water treated (L/g adsorbent) were 0.91 and 1.85, and the amount of nitrate removed (mg N/g adsorbent) were 9.8 and 22.2 for AG corn cob and Dowex-Fe, respectively, at a flux of 15 L/(m2/h).

Kalaruban|Mahatheva|M| Loganathan|Paripurnanda|P| Kandasamy|Jaya|J| Vigneswaran|Saravanamuthu|S|

Nitrate removal
Ion exchange resin
Bio-adsorbents
Membrane filtration/adsorption system
Transmembrane pressure


Prevalence of Documented Excessive Weight Gain Among Adolescent Girls and Young Women Using Depot Medroxyprogesterone Acetate.

Abstract:
Background
Depot medroxyprogesterone acetate (DMPA) is an accessible contraception with high efficacy among adolescents. However, concern of weight gain can impede DMPA use. The objective of this study was to determine prevalence of excessive weight gain associated with DMPA injection in young women and its predicting factor.

Methods
This retrospective chart review included young women aged 10 - 24 years who had used DMPA and attended at Family Planning and Reproductive Medicine Unit, Siriraj Hospital for at least 6-month period during January 2010 to June 2016. Baseline clinical data, weight at beginning of contraception, and weight at 6 and 12 months thereafter were reviewed. Excessive weight was defined as weight gain of > 5% of their baseline weight at 6 months. Various baseline variables were compared between groups with and without excessive weight gain.

Results
Among 231 DMPA users in this study, there were 28 women (12.1%, 95% confidence interval (CI): 7.8 - 16.3) who had an excessive weight gain at 6 months. Age, baseline body mass index, or race did not affect the likelihood of excessive weight gain. The excessive weight gain group had significant higher proportion of nulliparity, unmarried status and DMPA-ever used history compared to another one. Six of 13 (46.2%) excessive weight gainers at 6 months who continued DMPA use had gain even more weight (> 10% of their baseline weigh) at 12 months.

Conclusions
The majority of adolescent girls using DMPA had no excessive weight gain in 6 months. However, DMPA users who had excessive weight at 6 months were at high risk of gaining even more weight at 1 year.

Weight gain
Adolescent
Young women
DMPA
Contraception


25 Years of Light-Emitting Electrochemical Cells: A Flexible and Stretchable Perspective.

Abstract: Light-emitting electrochemical cells (LECs) are simple electroluminescent devices comprising an emissive material containing mobile ions sandwiched between two electrodes. The operating mechanism of the LEC involves both ionic and electronic transport, distinguishing it from its more well-known cousin, the organic light-emitting diode (OLED). While OLEDs have become a leading player in commercial displays, LECs have flourished in academic research due to the simple device architecture and unique features of its operating mechanism, inviting exploration of new materials and fabrication strategies. These explorations have brought LECs to an exciting frontier in advanced optoelectronics: flexible and stretchable light-emitting devices. Flexible and stretchable LECs are discussed herein, presenting the LEC system as a robust and fault-tolerant development platform. The engineering of emissive composites is highlighted to control mechanical properties, and how the tolerance of LECs to electrode work function and roughness has enabled the incorporation of new electrode materials to achieve flexibility and stretchability. As part of this story, the solution processability of LECs has led to exciting demonstrations of flexible and printed LECs. An outlook is provided for LECs that builds on these strengths, potentially leading to flexible, stretchable, low-cost devices such as illuminated tags, smart packaging, flexible signage, and wearable illumination.

light-emitting electrochemical cells
electroluminescence
flexible electronics
printed electronics
stretchable electronics


High serum potassium level is associated with successful electrical cardioversion for new-onset atrial fibrillation in the intensive care unit: A retrospective observational study.

Abstract: Electrical cardioversion (ECV) is a potentially life-saving treatment for haemodynamically unstable new-onset atrial fibrillation (AF); however, its efficacy is unsatisfactory. We aimed to elucidate the factors associated with successful ECV and prognosis in patients with AF. This retrospective observational study was conducted in two mixed intensive care units (ICUs) in a university hospital. Patients with new-onset AF who received ECV in the ICU were enrolled. We defined an ECV session as consecutive shocks within 15 minutes. The success of ECV was evaluated five minutes after the session. We analysed the factors associated with successful ECV and ICU mortality. Eighty-five AF patients who received ECV were included. ECV was successful in 41 (48%) patients, and 11 patients (13%) maintained sinus rhythm until ICU discharge. A serum potassium level ≥3.8 mol/L was independently associated with successful ECV in multivariate analysis (odds ratio (OR), 3.13; 95% confidence interval (CI), 1.07-9.11; p = 0.04). Maintenance of sinus rhythm until ICU discharge was significantly associated with ICU survival (OR 9.35; 95% CI 1.02-85.78, p = 0.048). ECV was successful in 48% of patients with new-onset AF developed in the ICU. A serum potassium level ≥3.8 mol/L was independently associated with successful ECV, and sinus rhythm maintained until ICU discharge was independently associated with ICU survival. These results suggested that maintaining a high serum potassium level may be important when considering the effectiveness of ECV for AF in the ICU.

Kyo|Michihito|M| Hosokawa|Koji|K| Ohshimo|Shinichiro|S| Kida|Yoshiko|Y| Tanabe|Yuko|Y| Ota|Kohei|K| Shime|Nobuaki|N|

D001281:Atrial Fibrillation
D004554:Electric Countershock
D006801:Humans
D007362:Intensive Care Units
D011188:Potassium
D012189:Retrospective Studies
D016896:Treatment Outcome

electrical cardioversion
atrial fibrillation
mortality
Critically ill
rhythm control
sepsis


Factors influencing the en route survivorship and post-voyage growth of a common ship biofouling organism, Bugula neritina.

Abstract: The likelihood that viable non-indigenous biofouling species will survive a voyage on a vessel is influenced by a range of factors, including the speed, duration, and route of the voyage and the amount of time the vessel spends in port. In this study, a land-based dynamic flow device was used to test the effect of recruit age, vessel speed and voyage duration on the survivorship and growth of the bryozoan Bugula neritina. In the experiment, one-week-old recruits had a higher likelihood (100%) of surviving voyages than older (one-month-old, 90%) or younger (one-day-old, 79%) recruits, but survival was not influenced by vessel speed (6 and 18 knots) or voyage duration (two and eight days). The results suggest that the non-indigenous species B. neritina can be effectively transferred at a range of ages but one-week-old recruits are more likely to survive the translocation process and survive in the recipient environment.

Schimanski|Kate B|KB|
Piola|Richard F|RF|
Goldstien|Sharyn J|SJ|
Floerl|Oliver|O|
Grandison|Clare|C|
Atalah|Javier|J|
Hopkins|Grant A|GA|

survivorship
Bryozoan
hull-fouling
marine invasion
voyage characteristics