Detection and Characterization of Membrane-Bound Selenoproteins in the Kidney of the Rat
The essential trace element selenium is of fundamental importance to human biology. Information on the membrane-bound selenoproteins was obtained by labeling of rats in vivo with 75Se-selenite, subcellular fractionation of the tissue homogenates, separation of the proteins by SDS-PAGE, two-dimensional electrophoresis, and selenium detection by autoradiography. In this way, in addition to already known membrane-bound selenoproteins three novel selenoproteins in the molecular mass range of 3840, 18, and 1213 kDa were detected in the microsomal membranes.
selenium two-dimensional electrophoresis membrane selenoproteins antioxidant