In silico and experimental validation of proteinprotein interactions between PknI and Rv2159c from Mycobacterium tuberculosis
PknI interacts with two novel proteins, Rv2159c and Rv0148. The three dimensional structure of Rv2159c was predicted. The in silico docking between PknI and Rv2159c was established well. The possible interacting residues were mutated and their structural stability were analyzed. PknI attracts the hydrophobic residues (AlaGlyTrp) for its interaction with Rv2159c.
proteinprotein interaction pkni homology modeling protein docking molecular dynamics binding free energy