Systems-Level Evidence of Transcriptional Co-Regulation of Yeast Protein Complexes
Protein complexes are functional units in the cell whose formation is regulated by a number of mechanisms. Previous studies have suggested that transcriptional co-regulation of protein components play a minor role in assembly of individual complexes. Recent developments in experimental approaches and bioinformatic analyses offer an opportunity to investigate the cell at a global or systems level with better quality. Here we show that, using genome/proteome-wide yeast data of protein complexes and protein-DNA interactions in normal growth conditions, a contribution of transcriptional co-regulation to global formation of protein complexes is statistically significant at a systems level. A significant fraction of proteins that form complexes shares common transcription factors on a global scale. We provide evidence for the significant contribution by quantifying transcriptional co-regulation of complexes as a global topological pattern of an integrated network. Biological relevance is examined by functional analysis of highly co-regulated complexes such as transport complexes. Our finding indicates that there exists linear causality at a systems level between transcriptional co-regulation of genes and formation of multi-protein complexes in the unicellular organism.
transcriptional co-regulation yeast protein complexes co-regulation measure systems-level non-randomness