Thymosin ?1 to harness immunity to pathogens after haploidentical hematopoietic transplantation
We designed a phase I/II clinical study to determine safety and efficacy of thymosin ?1 (T?1) administration in recipients of one HLA haplotype (haploidentical) stem cell transplants for hematologic malignancies. T?1 administration did not cause acute or chronic graft versus host disease and was associated with significant improvement in polymorphonuclear (phagocytosis) and dendritic cell (phagocytosis, expression of costimulatory molecules, and cytokine production) functions. It was also associated with increased T-cell counts and earlier appearance of functional pathogen-specific T cell responses (by a sensitive limiting dilution assay that detects frequency of T cells specific for Aspergillus, Candida, CMV, ADV, VZV, HSV, Toxoplasma). Five of six haploidentical transplant recipients who received T?1 are alive and disease free at a median follow-up of 10 months after transplantation (range: 520). They experienced only a single nonlethal infectious episode and one patient developed fatal immune hemolytic anemia. At this very early stage of the clinical trial, we conclude T?1 administration is safe and may impact favorably on immune function. Larger numbers of patients and longer follow-up are, of course, needed to assess its impact on survival.
thymosin ?1 hematopoietic transplantation haploidentical transplantation transplant-related mortality immune reconstitution