Regulation of Adrenoceptor and Muscarinic Receptor Gene Expression after Single and Repeated Stress
Although stress is tightly connected with elevated levels of catecholamines, stress effects on target structures of catecholamine actionadrenoceptors (ARs)has not been deeply studied yet. Similarly, very little is known about changes of muscarinic receptors (MRs) during stress. We determined changes in these receptors in the individual parts of the heart (right atria and ventricles) of animals (rats and mice) exposed to a single and repeated immobilization stress. Changes of tissue catecholamines, ?2-AR gene expression, protein levels, and binding sites were determined in rat right ventricles, and changes in ?1-, ?2-, and ?3-AR gene expression were followed in murine right atria. Tissue catecholamines were elevated, while ?2-AR mRNA levels and ?2-AR proteins and binding were decreased, in rat right ventricles. In murine right atria, ?1- and ?2-AR gene expression was elevated, while ?3-AR mRNA levels and M2-MR were reduced. Taken together, our data show that interaction of AR and MR is important for the organism coping with stress and that different heart regions reveal distinct reactions to stress.
adrenoceptors muscarinic receptors stress-heart