Analysis of in Vivo Endothelial Cell Activation Applying RT-PCR following Endothelial Cell Isolation by Laser Dissection Microscopy
Laser dissection microscopy was applied to isolate endothelial cells from tumors obtained from mice treated with TNF-?. RNA integrity was demonstrated from whole sections and dissected cells after acetone fixation and hematoxylin staining. RT-PCR for GAPDH, CD31, VCAM-1, ICAM-1, and E-selectin was successfully performed on these samples. These data demonstrate the feasibility of analyzing local endothelial cell responses in diseased tissues at the level of gene expression.
endothelial cell rt-pcr laser dissection microscopy inflammation angiogenesis cell adhesion molecules