Flow Cytometry to Evaluate Anaplasma marginale Parasitemia Using a Fluorescent Nucleic Acid Stain
In this work we describe a flow cytometrybased method using SYTO16 (a DNA intercalating agent) to quantify Anaplasma marginaleinfected erythrocytes in blood from bovine animals. The linearity and reproducibility of the results obtained with SYTO16 labeling followed by flow cytometry analysis make it a suitable approach for measurement of parasitemia in A. marginale infections.
flow cytometry anaplasma marginale parasitemia syto16