Mutations on Fibrinogen (?316322) Are Associated with Reduction in Platelet Adhesion Under Flow Conditions
In this paper we report on studies of platelet adhesion to several fibrinogen ? chain variants under physiological flow conditions. Reduced platelet adhesion was found to patient dysfibrinogen Vlissingen and its recombinant form (deletion of ?319320). Furthermore, substitutions of the amino acids 318, 320, or both in the recombinant fibrinogen ? chain showed a strong decrease in platelet adhesion under flow conditions in our perfusion system. Antibodies raised against peptides covering these sequences inhibited platelet adhesion completely, which suggested that the ?316322 sequence could be involved in platelet adhesion in flowing blood.
platelet adhesion flow conditions fibrinogen ? chain