A Polymorphic Form of Steroidogenic Factor 1 Associated with ACTH Receptor Deficiency in Mouse Adrenal Cell Mutants
We have described a family of adrenocortical tumor cell mutants (including clones OS3, Y6, and 10r9) that are resistant to ACTH because they fail to express the gene encoding the ACTH receptor (MC2R). The MC2R deficiency results from a mutation that impairs the activity of the nuclear receptor steroidogenic factor 1 (SF1) at the MC2R promoter. In this report, we show that ACTH resistance in the mutant clones is associated with a Sf1 gene that has Ser at codon 172 instead of Ala. In two of the three mutant clones, this Sf1 allele is amplified together with flanking DNA from chromosome 2 that includes the genes encoding germ cell nuclear factor and the beta-type proteosome subunit Psmb7. SF1A172 and SF1S172 exhibit little or no difference in transcriptional activity in SF1-dependent reporter gene assays, suggesting that SF1S172 per se is not directly responsible for the loss of MC2R expression. Instead, the Sf1S172 allele appears to be a marker of ACTH resistance in this family of adrenocortical tumor cell mutants, possibly reflecting the activity of a neighboring gene.
polymorphism steroidogenic factor 1 acth receptor chromosome 2 germ cell nuclear factor proteosome subunit psmb7 extrachromosomal dna gene amplification mc2 receptor y1 adrenocortical tumor cells