Resolution of infrared spectra and kinetic analysis of mutarotation of D-mannose in water
infrared spectra of a solution of D-mannose undergoing mutarotation were acquired at regular time intervals. The spectra were first-order differentiated and sequential rank analysis was used to obtain the concentration profiles of the anomers. Least-squares were then used to resolve the infrared spectra of the anomers. Rate constants for the conversion from alpha- to beta-D-mannose were calculated from the concentration profiles. The constants are slightly different from earlier reported values, while the equilibrium concentrations agree well with reported values. (C) 1999 Elsevier Science B.V. All rights reserved.
infrared spectra mutarotation d-mannose