A novel multi-scale Hessian based spot enhancement filter for two dimensional gel electrophoresis images
We introduce a novel spot filter for separating the closely overlapping protein spots. We compute the eigen components of the image Hessian of two overlapping spots. We evaluate the performance of the proposed filter on the synthetic and real images. Comparison with other methods and a commercial software package reveals the superior performance of the proposed filter.
hessian spot enhancement filter two-dimensional gel electrophoresis detection segmentation proteomics