Purification of Proteins Susceptible to Oxidation at Cysteine Residues: Identification of Malate Dehydrogenase as a Target for S-Glutathiolation
The cysteine residues of proteins are susceptible to a range of oxidative modifications, including S-glutathiolation. Here we present a study in which we used glutathione-agarose to purify cardiac proteins that undergo S-glutathiolation during oxidative stress. We then identified one of the oxidized proteins as malate dehydrogenase.
protein oxidation cysteine s-thiolation reperfusion heart