Structure-based grafting and identification of kinaseinhibitors to target mTOR signaling pathway as potential therapeutics for glioblastoma
A protocol is described to graft inhibitors from their cognate kinases to non-cognate mTOR. The grafted inhibitormTOR affinity is virtually evaluated using a consensus scoring strategy. A number of identified inhibitors are assayed to determine their inhibition against mTOR. Diverse nonbonded interactions are found at mTORinhibitor complex interface.
kinaseinhibitor mtor glioblastoma rational drug design