TGF-? stimulation in human and murine cells reveals commonly affected biological processes and pathways at transcription level
The TGF-? signaling pathway is a fundamental pathway in the living cell, which plays a key role in many central cellular processes. The complex and sometimes contradicting mechanisms by which TGF-? yields phenotypic effects are not yet completely understood. In this study we investigated and compared the transcriptional response profile of TGF-?1 stimulation in different cell types. For this purpose, extensive experiments are performed and time-course microarray data are generated in human and mouse parenchymal liver cells, human mesenchymal stromal cells and mouse hematopoietic progenitor cells at different time points. We applied a panel of bioinformatics methods on our data to uncover common patterns in the dynamic gene expression response in respective cells.
tgf-? microarray time-course analysis gene set analysis clustering functional similarity