Inhibition of TNF-?-induced Activation of NF-?B by Hantavirus Nucleocapsid Proteins
Hantaviruses cause two human diseases thought to be immune-mediated: hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). We recently reported that the nucleocapsid (N) protein of HFRS-causing Hantaan virus (HTNV) inhibits tumor necrosis factor-? (TNF-?) activation of nuclear factor-?B (NF-?B). Here we measured the ability of other hantaviral N proteins to similarly interfere with the inflammatory process of TNF-?. We found that like HTNV N, the N proteins of HFRS-causing Seoul and Dobrava viruses inhibited TNF-? activation of NF-?B and translocation of the NF-?B p65 subunit, but did not interfere with degradation of inhibitor of NF-?B (I?B). In contrast, the HFRS-causing Puumala virus and the HPS-causing Andes and Sin Nombre viruses did not prevent TNF-? activation of NF-?B or nuclear translocation of p65. These studies provide evidence that hantaviruses differ in their abilities to interfere with host innate immune responses.
nf-?b hantavirus nucleocapsid tnf-? importin ?