EasyGene  a prokaryotic gene finder that ranks ORFs by statistical significance
Contrary to other areas of sequence analysis, a measure of statistical significance of a putative gene has not been devised to help in discriminating real genes from the masses of random Open Reading Frames (ORFs) in prokaryotic genomes. Therefore, many genomes have too many short ORFs annotated as genes.
statistical significance computational gene finding hidden markov model short open reading frames automated genome annotation