Fabrication of nanostep for total internal reflection fluorescence microscopy to calibrate in water
Nanostep, whose refractive index was close to that of water, was obtained. The nanostep was fabricated via lift-off technique using PVA film and metal mask. The metal mask was transferred from a resin replica mold via nano transfer printing. The nanostep was made of a polymer resin and it was invisible in water. Using the nanostep, we succeeded in the calibration of the TIRF intensity in water.
tirf nanotransfer printing lift off technique polyvinyl alcohol index matching