Determination of Thymosin ?4 and Protein in Human Wound Fluid after Abdominal Surgery
Wound fluids were collected up to 60 h after abdominal surgery. Immediately after obtaining the wound fluid by Robinson drainage, wound fluid was centrifuged to remove blood cells and inflammatory cells. The concentration of total protein as well as of thymosin ?4 was determined in the cell-free supernatant solution. Total protein concentration decreased from about 50 g/L to 30 g/L within 60 h after surgery. After surgery we observed a concentration of up to 20 mg thymosin ?4 per liter decreasing to about 1 mg/L with time. Neither thymosin ?10 nor oxidized thymosin ?4 was detected in human wound fluid
human wound fluid surgery ?-thymosins time course