Detection of Coxiella burnetii in Market Chicken Eggs and Mayonnaise
We tried to detect C. burnetii in market chicken eggs and mayonnaise by nested PCR assay. The PCR target was the com 1 gene of C. burnetii. The positive rate for egg and mayonnaise samples was 4.2% and 17.6%, respectively. Direct sequence of some of the positive egg samples shows mutations whereas no mutation was found in the positive mayonnaise samples. The number of molecules of the Q fever agent is estimated at 104 to 106 per egg, according to our quantitative PCR test
coxiella burnetii egg mayonnaise nested pcr real-time pcr