Extração de DNA
Autor: EDP VI | Publicado: 2011-10-09T10:50:00-07:00

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Objetivos1. Obter ácido desoxirribonucléico (DNA) a partir de vegetais (morangos).2. Visualizar o DNA isolado.
Protocolo para isolamento de DNA vegetal (cebola ou morango)A. Material permanente- 1 Becker 200ml- 2 Beckers 500ml- 1 prato- 1Funil- Papel Filtro- garfo, faca e colher de sobremesa- ralador (se cebola)- recipiente para gelo ou banho-maria gelado- timer
B. Material para consumo- água quente a ~ 60°C- sal- detergente líquido - álcool gelado- gelo- 6 a 8 morangos ou 1 cebola média
&nbsp;C. Procedimentos1. Aquecer a água.2. Preparar tampão de extração em um dos Beckers de 500ml, misturando bem:- meio copo de água quente (~ 200ml)- 2 colheres de sal- 3 colheres de detergente3. Descascar e ralar a cebola, ou picar bem e esmigalhar os morangos (sem o cabo),usando o prato;4. Misturar bem a cebola ou o morango com o tampão de extração no próprio Becker;5. Esperar de 10 a 15 min;6. Esfriar a mistura colocando 2 a 3 cubos de gelo ao redor do Becker (ou em banhomariagelado);7. Esperar de 5 a 10 min;8. Filtrar a mistura para o outro Becker de 500ml usando o coador;9. Derramar vagarosamente álcool gelado nas bordas do Becker com a mistura filtradaaté formar uma camada de 2 a 3cm de álcool sobre a mistura;10. Após alguns instantes, o DNA começará a aparecer junto com bolhas e poderá serpescado com o garfo ou colher.