syedzayyan/paper_mill_benchmark huggingface dataset:
Trizbenzim, Cu-Trizbenzim and Zn-Trizbenzim as G-Quadruplex Inducing and Stabilizing Compounds on Human Telomeric Sequence and their Anticancer Properties.

The benzimidazole and their derivatives have rich biological relevance with respect to available natural amino acids and their role in protein folding and quaternary conformations.Thus the ligand trizbenzim and their cu(ii) and zn(ii) metal complexes were prepared to induce g-quadruplex conformation even under no-salt conditions with remarkable anticancer activities.Methods the ligand n,n',n''-tris-(1h-benzoimidazol-2-ylmethyl)-[1,3,5]triazine-2,4,6-triamine ( trizbenzim) and its cu and zn complexes (cu-trizbenzim, zn-trizbenzim) were synthesized and characterized by ir, nmr, and maldi-tof techniques.The pure ligand and its complexes interacted with human telomere dna sequence d(ttaggg), htelo8and htelo20and the interactions were followed by circular dichroism spectroscopy, fid assay, and molecular docking techniques.The compounds were tested for anticancer activity towards selected cell lines.Results all the three compounds stabilized the htelo8 and htelo20 in parallel and antiparallel g-quadruplex conformations with salt conditions.Under no-salt conditions, the compounds induce and stabilize the g-quadruplex conformation in antiparallel topology selectively.The pure ligand, cu-trizbenzim, and zn-trizbenzim were involved in partial or classical intercalation and some backbone interactions on the strand.The fid assay using thiazole orange intercalator supports the proposed intercalation mode of binding for the three compounds, especially for the pure ligand and the cu-complex.The moe docking experiments using x-ray and nmr derived g-quadruplex models with the title compounds extensively support the g-quadruplex induction and stabilization of the telomere sequence by these compounds.The guanines bases involved in the g-tetrad formation interact well with the triazine and the benzimidazole part of the ligand through strong π-π interactions.The primary mode of binding is described as end stacking and intercalation of the compounds to the g-quadruplex structures.The cu-trizbenzim exhibited more anticancer property in comparison to the pure ligand and the zntrizbenzim complex.The ic50values were in the nanomolar range from 50 to 150n m in concentration.Conclusion this novel self-induction of g-quadruplex is novel without the presence of alkali metal ions.

Journal: Anti-cancer agents in medicinal chemistry (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
NKX3.1 Localization to Mitochondria Suppresses Prostate Cancer Initiation.

Mitochondria provide the first line of defense against the tumor-promoting effects of oxidative stress.Here we show that the prostate-specific homeoprotein nkx3.1 suppresses prostate cancer initiation by protecting mitochondria from oxidative stress.Integrating analyses of genetically engineered mouse models, human prostate cancer cells, and human prostate cancer organotypic cultures, we find that, in response to oxidative stress, nkx3.1 is imported to mitochondria via the chaperone protein hspa9, where it regulates transcription of mitochondrial-encoded electron transport chain (etc) genes, thereby restoring oxidative phosphorylation and preventing cancer initiation.Germline polymorphisms of nkx3.1associated with increased cancer risk fail to protect from oxidative stress or suppress tumorigenicity.Low expression levels of nkx3.1combined with low expression of mitochondrial etc genes are associated with adverse clinical outcome, whereas high levels of mitochondrial nkx3.1 protein are associated with favorable outcome.This work reveals an extranuclear role for nkx3.1 in suppression of prostate cancer by protecting mitochondrial function.Significance: our findings uncover a nonnuclear function for nkx3.1 that is a key mechanism for suppression of prostate cancer.Analyses of the expression levels and subcellular localization of nkx3.1 in patients at risk of cancer progression may improve risk assessment in a precision prevention paradigm, particularly for men undergoing active surveillance.see related commentary by finch and baena, p.2132.this article is highlighted in the in this issue feature, p.2113.

Journal: Cancer discovery (2021)
Publication types: Journal Article;Research Support, N.I.H., Extramural;Research Support, Non-U.S. Gov't;Research Support, U.S. Gov't, Non-P.H.S.
Risk factors for re-dislocation after closed reduction in children with developmental dysplasia of the hip.

To investigate the risk factors for re-dislocation after the closed reduction in children with developmental dysplasia of the hip (ddh).Methods the clinical data of 88 children aged ≤ 18 months with ddh (103 hips) who were treated with adductor muscle relaxation + closed reduction + plaster fixation at the children's hospital, zhejiang university school of medicine from january 2015 to december 2017, were retrospectively analyzed.According to the diagnostic criteria of hip dislocation, patients were divided into two groups: reduction group and re-dislocation group.The univariate and multivariate logistic regression analysis were applied to identify the risk factors for the re-dislocation of children.Results eighty-six patients (99 hips) successively underwent the treatment.69 hips were fixed at the first intention, 9 hips at the second intention, and a total of 78 hips with no re-dislocation occurred till the last follow-up with a rate of 78.8%.The univariate analysis showed that preoperative acetabular index (ai), international hip dysplasia institute (ihdi) grade, intraoperative hip flexion angle, and intraoperative head-socket spacing were significantly related to the occurrence of re-dislocation after closed-reduction.The multivariate logistic regression analysis showed that preoperative ai > 40.5° (or=5.57,p 6.95 mm (or=8.42,p 40.5°, flexion angle 6.95 mm, and ihdi grade were used to predict the occurrence of re-dislocation, and the sensitivity and specificity were 0.72 and 0.87, respectively.Conclusions preoperative ai > 40.5°, intraoperative hip flexion angle 6.95 mm are risk factors for postoperative re-dislocation in children with ddh.These risk factors combining with the ihdi grade would be better to predict the occurrence of re-dislocation.

Journal: Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences (2022)
Publication types: Journal Article
3D printed hydrogel scaffolds combining glutathione depletion-induced ferroptosis and photothermia-augmented chemodynamic therapy for efficiently inhibiting postoperative tumor recurrence.

Surgical resection to achieve tumor-free margins represents a difficult clinical scenario for patients with hepatocellular carcinoma.While post-surgical treatments such as chemotherapy and radiotherapy can decrease the risk of cancer recurrence and metastasis, growing concerns about the complications and side effects have promoted the development of implantable systems for locoregional treatment.Herein, 3d printed hydrogel scaffolds (designed as gel-sa-cu o) were developed by incorporating one agent with multifunctional performance into implantable devices to simplify the fabrication process for efficiently inhibiting postoperative tumor recurrence.Cu o nanoparticles can be effectively controlled and sustained released during the biodegradation of hydrogel scaffolds.Notably, the released cu o nanoparticles not only function as the reservoir for releasing cu2+to produce intracellular reactive oxygen species (ros) but also serve as photothermal agent to generate heat.Remarkably, the heat generated by photothermal conversion of cu o nanoparticles further promotes the efficiency of fenton-like reaction.Additionally, ferroptosis can be induced through cu2+-mediated gsh depletion via the inactivation of gpx4.By implanting hydrogel scaffolds in the resection site, efficient inhibition of tumor recurrence after primary resection can be achieved in vivo.Therefore, this study may pave the way for the development of advanced multifunctional implantable platform for eliminating postoperative relapsable cancers.

Journal: Journal of nanobiotechnology (2022)
Publication types: Journal Article
Ethanol-Induced Hydrogen Insertion in Ultrafine IrPdH Boosts pH-Universal Hydrogen Evolution.

Engineering pt-free catalysts for hydrogen evolution reaction (her) with high activity and stability is of great significance in electrochemical hydrogen production.Herein, in situ chemical h intercalation into ultrafine pd to activate this otherwise her-inferior material to form the ultrafine ir pd h hydride as an efficient and stable her electrocatalyst is proposed.The formation of pd ir h depends on a new hydrogenation strategy via using ethanol as the hydrogen resource.It is demonstrated that h atoms in ir pd h originate from the oh and ch2 of ethanol, which fills the gap of ethanol as the hydrogen source for the preparation of pd hydride.Thanks to the incorporation of h/ir atoms and ultrafine structure, the ir pd h exhibits superior her activity and stability in the whole p h range.The ir pd h delivers very low overpotentials of 14, 25 and 60 m v at a current density of 10 m a cm-2respectively in 0.5 m h2so4, 1 m koh, and 1 m pbs electrolytes, which are much better than those of commercial pt/c and most reported noble metal electrocatalysts.Theoretical calculations confirm that interstitial hydrogen availably refines the electronic density of pd and ir sites, which optimizes the adsorption of *h and leads to the significant enhancement of her performance.

Journal: Small (Weinheim an der Bergstrasse, Germany) (2022)
Publication types: Journal Article
HDAC1 promoted migration and invasion binding with TCF12 by promoting EMT progress in gallbladder cancer.

The identification of prognostic markers for gallbladder cancer is needed for clinical practice.Histone deacetylases (hdacs) play an important role in tumor development and progression by modifying histone and non-histone proteins.However, the expression of hdac1 in patients with gallbladder cancer is still unknown.Here, we reported that hdac1 expression was elevated in cancerous tissue and correlated with lymph node metastasis and poorer overall survival in patients with gbc.Knockdown of hdac1 using lentivirus delivery of hdac1-specific sh rna abrogated the migration and invasion of gbc cells in vitro.Tcf-12, as the hdac1 binding protein, has also correlates with poor prognosis in gbc patients.And there is a positive correlation between hdac1 and tcf-12 which leading the high invasion and migration ability of gbc cells.Taken together, our data suggested that hdac1 and tcf-12 are a potential prognostic maker and may be a molecular target for inhibiting invasion and metastasis in gbc.

Journal: Oncotarget (2016)
Publication types: Journal Article
Back to Normal?

The massive upheaval that the covid-19 pandemic has brought to all our lives has caused many of us to think long and hard about what kind of future we want and need.

Journal: Nursing for women's health (2021)
Publication types: Editorial
Cellulose Nanocrystals from Fibers of Macauba (Acrocomia Aculeata) and Gravata (Bromelia Balansae) from Brazilian Pantanal.

Cellulose nanocrystals (cnc) were obtained from macauba and gravata fibers.Macauba (or bocaiuva) is a palm tree found throughout most of brazil and gravata is an abundant kind of bromelia with 1-2m long leaves, found in brazilian pantanal and cerrado.The raw fibers of both fibers were mercerized with na oh solutions and bleached; they were then submitted to acid hydrolysis using h2so4at 45 °c, varying the hydrolysis time from 15 up to 75 min.The fibers were analyzed by x-ray diffraction (xrd), ftir spectroscopy, scanning electron microscopy (sem) and thermal stability by thermogravimetric analysis (tg).Xrd patterns did not present changes in the crystal structure of cellulose after mercerization, but it was observed a decrease of hemicellulose and lignin contents, and consequently an increase of cellulose content with the increase of na oh solution concentration in the mercerization.After acid hydrolysis, the cellulose nanocrystals (cnc) were also analyzed by transmission electron microscopy (tem) which showed an acicular or rod-like aspect and nanometric dimensions of cnc from both fibers, but the higher values of aspect ratio (l/d) were found on cnc obtained from gravata after 45 min of acid hydrolysis.The mercerization and subsequent bleaching of fibers influenced the crystallinity index and thermal stability of the resulting cnc, but their properties are mainly influenced by the hydrolysis time, i.E., there is an increase in crystallinity and thermal stability up to 45 min of hydrolysis, after this time, both properties decrease, probably due to the cellulose degradation by the sulfuric acid.

Journal: Polymers (2019)
Publication types: Journal Article
Quality improvement efforts in a safety net institution: Increased diabetes screening is associated with lower HbA1c at diagnosis and improved HbA1c outcomes in youth with type 2 diabetes.

S evaluate whether increased diabetes screening in youth is associated with lower hb a1c at t2d diagnosis and improved hb a1c outcomes in youth.Research design and methods diabetes screening rates from 2009 to 2018 were calculated.Electronic medical records identified obese youth ages 8-18 with first hb a1c ≥6.5% from 2009 to 2018; chart review confirmed incident t2d.Demographics, bmi and hb a1c values, and use of glucometer and diabetes medications were collected.Results 142 youth had t2d.Median age was 14 years (range 8-18); 58% were female.46% were identified on first hb a1c testing.69 (49%) had 1st hb a1c 6.5%-6.9%, 43 (30%) 7.0%-7.9%, and 30 (21%) ≥8%.Follow-up from 1st to last hb a1c was median 2.6 years (range 0-10).121 youth had follow-up testing ≥1 year after diagnosis; of these, 87 (72%) had persistent t2d-range hb a1c or were taking diabetes medications.85% of youth with 1st hb a1c ≥7% had persistent t2d versus 52% of those with 1st hb a1c <7% (p < 0.001).Poorly controlled diabetes at last test was present in 19% of youth with baseline hb a1c 6.5%-6.9%, 30% with 7.0%-7.9%, and 63% with ≥8% (p < 0.001).47 (68%) with hb a1c <7% were prescribed a glucometer; 9% of youth prescribed a meter and 41% of youth not prescribed a meter had poorly controlled diabetes at last test (p = 0.009).Conclusions youth with hb a1c <7% at diagnosis were less likely to have poorly controlled diabetes at follow-up.Prescription of glucometers for youth with hb a1c in this range was associated with improved hb a1c outcomes and deserves further study including components of glucometer teaching.

Journal: Pediatric diabetes (2022)
Publication types: Journal Article
Bipedal Walking of Underwater Soft Robot Based on Data-Driven Model Inspired by Octopus.

The soft organisms in nature have always been a source of inspiration for the design of soft arms and this paper draws inspiration from the octopus's tentacle, aiming at a soft robot for moving flexibly in three-dimensional space.In the paper, combined with the characteristics of an octopus's tentacle, a cable-driven soft arm is designed and fabricated, which can motion flexibly in three-dimensional space.Based on the tensor flow framework, a data-driven model is established, and the data-driven model is trained using deep reinforcement learning strategy to realize posture control of a single soft arm.Finally, two trained soft arms are assembled into an octopus-inspired biped walking robot, which can go forward and turn around.Experimental analysis shows that the robot can achieve an average speed of 7.78 cm/s, and the maximum instantaneous speed can reach 12.8 cm/s.

Journal: Frontiers in robotics and AI (2022)
Publication types: Journal Article
MicroRNA-4423-3p inhibits proliferation of fibroblast-like synoviocytes by targeting matrix metalloproteinase 13 in rheumatoid arthritis.

Rheumatoid arthritis (ra) is a chronic inflammatory autoimmune disease that is increasing in incidence worldwide.Ra is regulated by a variety of micro rnas (mi rnas/mi r).Moreover, analysis of public data has revealed that mi r-4423-3p is significantly downregulated in peripheral blood mononuclear cells of ra patients.This study investigated the role of mi r-4423-3p in ra.The levels of mi r-4423-3p and matrix metalloproteinase 13 (mmp13) in ra patients and the regulatory relationship between mi r-4423-3p and mmp13 were analyzed using public data.A dual-luciferase reporter assay was performed to verify that mi r-4423-3p targets mmp13 in human fibroblast-like synoviocyte (hfls) ra cells (hfls-ra).Following the overexpression of mi r-4423-3p, mi r-4423-3p inhibitor, and mmp13 in hfls-ra, viability, proliferation, cell cycle, apoptosis, and invasion/migration assays were used to detect the effects of mi r-4423-3p targeting mmp13 on cell biological processes.The results revealed that mi r-4423-3p was downregulated in peripheral blood mononuclear cells of ra patients and mmp13 was upregulated in synovial tissue of ra patients.Mi r-4423-3p targets the 3' untranslated region of mmp13 and downregulates mmp13 expression.After overexpression of mi r-4423-3p, cell proliferation, migration, and invasion were inhibited, the cell cycle was prevented and cell apoptosis was promoted.Overexpression of mmp13 promoted cell proliferation, migration, and invasion, while accelerating the cell cycle process and suppressing apoptosis.The findings indicate that in hfls-ra cells, overexpression of mi r-4423-3p inhibited proliferation, migration, and invasion, and promoted apoptosis by negatively regulating mmp13.The overexpression of mi r-4423-3p might be a novel strategy for the treatment of ra.

Journal: Bioengineered (2021)
Publication types: Journal Article;Video-Audio Media
Impact of Overweight and Obesity on US Papillary Thyroid Cancer Incidence Trends (1995-2015).

Since the early 1980s, papillary thyroid cancer (ptc) incidence rates and the prevalence of obesity, a risk factor for ptc, have increased substantially in the united states.We estimated the proportion of ptc incidence in the united states attributable to overweight and obesity during 1995-2015.Methods national institutes of health-aarp diet and health study cohort data (n = 457 331 participants, 50-71 years and cancer-free at baseline) were used to estimate multivariable-adjusted hazard ratios (hrs) for ptc across body mass index categories.Population attributable fractions (pafs) were calculated using estimated hazard ratios and annual overweight and obesity prevalence estimates from the national health interview survey.Paf estimates were combined with surveillance, epidemiology, and end results-13 data to calculate annual percent changes in ptc incidence rates attributable (and unrelated) to overweight and obesity.Results overweight (25.0-29.0 kg/m2) and obesity (≥30.0 kg/m2) were associated with 1.26-fold (95% confidence interval [ci] = 1.05- to 1.52-fold) and 1.30-fold (95% ci = 1.05- to 1.62-fold) increased risks of ptc, respectively, and nearly threefold (hr = 2.93, 95% ci = 1.25 to 6.87) and greater than fivefold (hr = 5.42, 95% ci = 2.24 to 13.1) increased risks of large (>4 cm) ptcs compared with normal weight (18.5-24.9 kg/m2).During 1995-2015, paf estimates for overweight and obesity increased from 11.4% to 16.2% for all ptcs and from 51.4% to 63.2% for large ptcs.Overweight or obesity accounted for 13.6% and 57.8% of the annual percent changes in total (5.9%/y) and large (4.5%/y) ptc incidence rates, respectively, during 1995-2015.Conclusions overweight and obesity may have contributed importantly to the rapid rise in ptc incidence during 1995-2015.By 2015, we estimate that one of every six ptcs diagnosed among adults 60 years or older, including nearly two-thirds of large ptcs, were attributable to overweight and obesity.

Journal: Journal of the National Cancer Institute (2020)
Publication types: Journal Article;Research Support, N.I.H., Intramural
Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications.

A simple procedure to incorporate enzymes (horseradish peroxidase, hrp, and lactate oxidase, lox) within alginate hydrogels is reported with electrochemiluminescence (ecl) used to detect the enzymatic reactions with the corresponding substrates.First, hrp and lox were successfully immobilized into ca co3microspheres, followed by the electrostatic layer-by-layer deposition of a nanoshell onto the microspheres, and finally by their dispersion into alginate solution.The as-prepared dispersion was drop cast onto the glassy carbon electrodes and cross-linked by the external and internal gelation methods using ca2+cations.The enzymes encapsulated within the alginate hydrogels were characterized using cyclic voltammetry and kinetic studies performed using ecl.The results showed that the enzymatic activity was significantly maintained as a result of the immobilization, with values of the apparent michaelis-menten constants estimated as 7.71 ± 0.62 and 8.41 ± 0.43 μm, for hrp and lox, respectively.The proposed biosensors showed good stability and repeatability with an estimated limit of detection of 5.38 ± 0.05 and 0.50 ± 0.03 μm for hydrogen peroxide and lactic acid, respectively.The as-prepared enzymes encapsulated within the alginate hydrogels showed good stability up to 28 days from their preparation.The sensitivity and selectivity of the enzymes encapsulated within the alginate hydrogels were tested in real matrices (hrp, hydrogen peroxide, in contact lens solution; lox, lactic acid in artificial sweat) showing the sensitivity of the ecl detection methods for the detection of hydrogen peroxide and lactic acid in real samples.

Journal: Analytical chemistry (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Detection of adulterants in grape nectars by attenuated total reflectance Fourier-transform mid-infrared spectroscopy and multivariate classification strategies.

There is no any doubt about the importance of food fraud control, as it has implications in food safety and in consumer health.Focusing on fruit beverages, some types of adulterations have been detected more frequently, such as substitution with less expensive fruits.A methodology based on attenuated total reflectance fourier-transform mid-infrared spectroscopy (atr-ftir) and multivariate classification was applied to detect whether grape nectars were adulterated by substitution with apple juice or cashew juice.A total of 126 samples were obtained and analyzed.Two strategies were proposed: one-class and multiclass approaches.Soft independent modeling of class analogy (simca), partial least squares discriminant analysis (pls-da) and partial least squares density modeling (pls-dm) were used to build the models.Among them, pls-da presented the best performance with a sensitivity and specificity of nearly 100%.The multiclass strategy was preferred if the adulterants to be studied are known because it provides additional information.

Journal: Food chemistry (2018)
Publication types: Journal Article