syedzayyan/paper_mill_benchmark huggingface dataset:
SARS-CoV-2 Seroprevalence Post-First Wave among Primary Care Physicians in Catania (Italy).

Family physicians or pediatricians and general practitioners (gps) work in non-hospital settings.Gps usually visit many patients, frequently at their homes, with low potential, if any, to control the work setting.Particularly during the initial phases of the covid-19 outbreak, they were not informed about the occurrence of sars-co v-2-infected patients, with inadequate information regarding the risk, a lack of suitable protective measures and, in some cases, deficient or poor accessibility to personal protective equipment (ppe).During the first wave of covid-19, primary care physicians were on the front line and isolated the first cases of the disease.The present study aims to estimate the seroprevalence of sars-co v-2 in a cohort of 133 gps working in catania (italy) after the first wave of covid-19.Serological analysis revealed a low seroprevalence (3%) among gps.The low seroprevalence highlighted in the results can be attributed to correct management of patients by gps in the first wave.It is now hoped that mass vaccination, combined with appropriate behavior and use of ppe, can help further reduce the risk of covid-19 disease.

Journal: Tropical medicine and infectious disease (2021)
Publication types: Journal Article
First Survey of the Pathogenic Fungus Batrachochytrium dendrobatidis in Wild Populations of the Yunnan Caecilian (Ichthyophis bannanicus) in Guangxi, China.

Batrachochytrium dendrobatidis (bd), which causes chytridiomycosis, mainly infects anura and caudata but is poorly known in gymnophiona.We conducted a survey of bd in the yunnan caecilian (ichthyophis bannanicus) and found that 6 of 71 samples (8.4%) tested positive for bd.To our knowledge, this is the first detection of bd in wild i.Bannanicus.

Journal: Journal of wildlife diseases (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Quantifying Rheumatoid Arthritis Disease Activity Using a Multimodal Sensing Knee Brace.

Rheumatoid arthritis (ra) is a chronic inflammatory syndrome that features painful and destructive joint disease.Aggressive disease-modifying treatment can result in reduced symptoms and protection from irreversible joint damage; however, assessment of treatment efficacy is currently based largely on subjective measures of patient and physician impressions.In this work, we address this compelling need to provide an accurate and quantitative capability for monitoring joint health in patients with ra.Methods joint acoustic emissions (jaes), electrical bioimpedance (ebi), and kinematics were measured noninvasively from 11 patients with ra over the course of three weeks using a custom multimodal sensing brace, resulting in 49 visits with jae recordings and 43 with ebi recordings.Features derived from all sensing modalities were fed into a linear discriminant analysis (lda) model to predict disease activity according to the validated disease activity index (the das28-esr).Erythrocyte sedimentation rate (esr) was predicted using ridge regression and classified into a high or low class using lda.Results das28-esr level was predicted with an area under the receiver operating characteristic curve (auc) of 0.82.With jaes alone, we were able to track intrasubject differences in the disease activity score as well as classify esr level with an auc of 0.93.The majority of patients reported both an interest and ability to use the brace at home for longitudinal monitoring.Conclusion this work demonstrates the ability to detect ra disease activity using noninvasive sensing.Significance this system has the potential to improve ra disease activity monitoring by giving treating clinicians objective data that can be acquired independent of a face-to-face clinic visit.

Journal: IEEE transactions on bio-medical engineering (2022)
Publication types: Journal Article;Research Support, U.S. Gov't, Non-P.H.S.
Ogerin mediated inhibition of TGF-β(1) induced myofibroblast differentiation is potentiated by acidic pH.

Transforming growth factor beta (tgf-β) induced myofibroblast differentiation is central to the pathological scarring observed in idiopathic pulmonary fibrosis (ipf) and other fibrotic diseases.Our lab has recently identified expression of gpr68 (ovarian cancer gene receptor 1, ogr1), a p h sensing g-protein coupled receptor, as a negative regulator of tgf-β induced profibrotic effects in primary human lung fibroblasts (phlfs).We therefore hypothesized that small molecule activators of gpr68 would inhibit myofibroblast differentiation.Ogerin is a positive allosteric modulator (pam) of gpr68, inducing a leftward shift of the dose response curve to proton induced signaling.Using phlfs derived from patients with both non-fibrotic and ipf diagnoses, we show that ogerin inhibits, and partially reverses tgf-β induced myofibroblast differentiation in a dose dependent manner.This occurs at the transcriptional level without inhibition of canonical tgf-β induced smad signaling.Ogerin induces pka dependent creb phosphorylation, a marker of gαs pathway activation.The ability of ogerin to inhibit both basal and tgf-β induced collagen gene transcription, and induction of gαs signaling is enhanced at an acidic p h (p h 6.8).Similar findings were also found using fibroblasts derived from dermal, intestinal, and orbital tissue.The biological role of gpr68 in different tissues, cell types, and disease states is an evolving and emerging field.This work adds to the understanding of gαs coupled gpcrs in fibrotic lung disease, the ability to harness the p h sensing properties of gpr68, and conserved mechanisms of fibrosis across different organ systems.

Journal: PloS one (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't;Research Support, N.I.H., Extramural
A Novel Ferroptosis-Related LncRNA Pair Prognostic Signature Predicts Immune Landscapes and Treatment Responses for Gastric Cancer Patients.

The construction of ferroptosis-related lnc rna prognostic models in malignancies has been an intense area of research recently.However, most of the studies focused on the exact expression of lnc rnas and had limited application values.Herein, we aim to establish a novel prognostic model for gastric cancer (gc) patients and discuss its correlation with immune landscapes and treatment responses.methods:the present study retrieved transcriptional data of gc patients from the cancer genome atlas (tcga) database.We identified differentially expressed ferroptosis-related lnc rnas between tumor and normal controls of gc samples.Based on a new method of cyclically single pairing, we constructed a 0 or 1 matrix of ferroptosis-related lnc rna pairs (frlps).A risk score signature consisting of 10 frlps was established using multi-step cox regression analysis.Next, we performed a series of systematic analyses to investigate the association of the frlp model and tumor microenvironment, biological function, and treatment responses.An alternative model to the frlp risk score signature, the gene set score (gs) model was also constructed, which could represent the former when lnc rna expression was not available.results:we established a novel prognostic signature of 10 ferroptosis-related lnc rna pairs.High-risk patients in our risk score model were characterized by high infiltration of immune cells, upregulated carcinogenic and stromal activities, and heightened sensitivity to a wide range of anti-tumor drugs, whereas low-risk patients were associated with better responses to methotrexate treatment and elevated immunotherapeutic sensitivity.The practicability of the frlp risk score model was also validated in two independent microarray datasets downloaded from gene expression omnibus (geo) using the gs model.Finally, two online dynamic nomograms were built to enhance the clinical utility of the study.conclusion:in this study, we developed a ferroptosis-related lnc rna pair-based risk score model that did not rely on the exact lnc rna expression level.This novel model might provide insights for the accurate prediction and comprehensive management for gc patients.

Journal: Frontiers in genetics (2022)
Publication types: Journal Article
The Effectiveness of a Critical Appraisal Skills Workshop 6 Months After Nurses' Participation.

The aim of this study was to explore whether nursing staff retained knowledge, self-confidence, and attitudes 6 months after participating in a critical appraisal skills workshop.Method a retrospective matched case-control study was conducted to ascertain the effects of this training on the ability to appraise literature.Results mean scores on the critical appraisal knowledge test, self-confidence, and attitudes were significantly higher in the case group compared to the control group (p< .001,p< .05, andp= .031, respectively).Conclusion six months after the workshop, critical appraisal knowledge, self-confidence, and attitudes remained improved among the nursing staff who participated.This indicates that the workshop was effective for learning and can be used for clinical training.[j contin educ nurs.2022;53(8):372-378.].

Journal: Journal of continuing education in nursing (2022)
Publication types: Journal Article
Family resources and school performance: an analysis of associated factors in middle school adolescents.

To verify the association between family resources, sociodemographic aspects, and school performance in middle school students.Methods observational, analytical, cross-sectional study with a probabilistic sample stratified by sex, age, and grade in school.A total of 124 adolescents of both sexes, aged 11 to 14 years, enrolled in a private middle school participated in the study.We sent to their parents/guardians an online form with the home environment resources scale (her), brazilian economic classification criteria (cceb), and sociodemographic questions.The school performance was furnished by the institution.We used frequency distribution analysis of categorical variables and measures of central tendency and dispersion of the continuous ones.We used the mann-whitney, kruskal-wallis, and spearman correlation coefficient tests and set the significance level at p ≤ 0.05.Results most of the sample was 11 years old, females, sixth graders, with very good school performance, belonging to social class a, and whose householder had a bachelor's degree.Age and grade in school were associated with interaction with the parents, "family-school connection", and total her score.The "proximal processes" had a moderate positive correlation with outings and toys.There was a moderate positive correlation between total her score and "predictable activities that indicate stability".The "proximal processes" had a strong positive correlation with "family-school connection".There was a weak positive correlation between books available at home and school performance.Conclusion the family resources were related to the adolescents' school performance.

Journal: CoDAS (2022)
Publication types: Journal Article;Observational Study
Vascular procedures in patients with left ventricular assist devices: single-center experience.

A growing number of patients suffering from heart failure is living with a left ventricular assist device (lvad) and is in the need for non-cardiac surgery.Vascular procedures due to ischemia, bleeding, or other device-related complications may be required and pose a challenge to the caregivers in terms of monitoring and management of these patients.Therefore, we reviewed our experience with lvad patients undergoing vascular surgery.Methods from january 2010 until march 2017, a total of 54 vascular procedures were performed on 41 lvad patients at our institution.Patient records were reviewed retrospectively in terms of incidence of lvad-related complications, including thrombosis, stroke, bleeding, wound healing, and survival associated with vascular surgery.The type of surgery was recorded, as well as various clinical demographic variables.Results vascular procedures were performed in 35 men (85.4%) and 6 women (14.6%) with lvads.There were no perioperative strokes, device thromboses, or device malfunctions.Thirty-day mortality overall was 26.8% (eleven patients), with most patients dying within 30 days after lvad implantation due to multi-organ failure.In 25 procedures (46.3%), a blood transfusion was necessary.Conclusion patients on lvad support are a complex cohort with a high risk for perioperative complications.In a setting where device function and anticoagulation are monitored closely, vascular surgery in these patients is feasible with an acceptable perioperative risk.

Journal: Indian journal of thoracic and cardiovascular surgery (2021)
Publication types: Journal Article
An acetylated mannan isolated from Aloe vera induce colorectal cancer cells apoptosis via mitochondrial pathway.

The anti-cancer effects of aloe vera barbadensis extract c (avbec) have been demonstrated in a previous study.However, the specific functional ingredient and mechanism remain undefined.This study aimed to evaluate the function and associated mechanisms of purified polysaccharide (abpa1) from avbec on colorectal cancer.Here, we identify that abpa1 can induce colorectal cancer apoptosis.In vivo, abpa1 significantly suppressed tumor growth in an orthotopic colon cancer model.Mechanistically, abpa1 alters mitochondrial membrane permeability by promoting bax translocation while causing cytochrome-c release, which initiates the caspase cascade reaction.Additionally, we found that abpa1 exerted distinct impacts on the mitochondrial metabolism of colorectal cancer cells.Our study elucidated the mechanism by which the polysaccharide abpa1 induces apoptosis in colorectal cancer cells through the regulation of bax and cytochrome-c mediated mitochondrial pathway, indicating that abpa1 may be developed as a mitochondrial-targeting anti-cancer drug.

Journal: Carbohydrate polymers (2022)
Publication types: Journal Article
Highly tailorable gellan gum nanoparticles as a platform for the development of T cell activator systems.

T cell priming has been shown to be a powerful immunotherapeutic approach for cancer treatment in terms of efficacy and relatively weak side effects.Systems that optimize the stimulation of t cells to improve therapeutic efficacy are therefore in constant demand.A way to achieve this is through artificial antigen presenting cells that are complexes between vehicles and key molecules that target relevant t cell subpopulations, eliciting antigen-specific t cell priming.In such t cell activator systems, the vehicles chosen to deliver and present the key molecules to the targeted cell populations are of extreme importance.In this work, a new platform for the creation of t cell activator systems based on highly tailorable nanoparticles made from the natural polymer gellan gum (gg) was developed and validated.Methods gg nanoparticles were produced by a water in oil emulsion procedure, and characterized by dynamic light scattering, high resolution scanning electronic microscopy and water uptake.Their biocompatibility with cultured cells was assessed by a metabolic activity assay.Surface functionalization was performed with anti-cd3/cd28 antibodies via edc/nhs or neutr avidin/biotin linkage.Functionalized particles were tested for their capacity to stimulate cd4+t cells and trigger t cell cytotoxic responses.Results nanoparticles were approximately 150 nm in size, with a stable structure and no detectable cytotoxicity.Water uptake originated a weight gain of up to 3200%.The functional antibodies did efficiently bind to the nanoparticles, as confirmed by sds-page, which then targeted the desired cd4+populations, as confirmed by confocal microscopy.The developed system presented a more sustained t cell activation over time when compared to commercial alternatives.Concurrently, the expression of higher levels of key cytotoxic pathway molecules granzyme b/perforin was induced, suggesting a greater cytotoxic potential for future application in adoptive cancer therapy.Conclusions our results show that gg nanoparticles were successfully used as a highly tailorable t cell activator system platform capable of t cell expansion and re-education.

Journal: Biomaterials research (2022)
Publication types: Journal Article
Seroprevalence of Wenzhou virus in China.

Wenzhou virus (wenv) was first identified in rodents and asian house shrews in wenzhou, zhejiang province, china.However, little is known about the prevalence of wenv infections in humans in china.To determine the threat that wenv may pose to humans, we determine the seroprevalence of wenv in healthy individuals in china in this study.Cross-reactivities of nucleoprotein (np) were detected between lymphocytic choriomeningitis virus (lcmv) and wenv using western blot and elisa assy.The prevalence of specific ig g antibodies against wenv np was investigated in different age groups of 830 healthy individuals aged 0-70 years old in china using a competition elisa assay.The results indicate that wenv and lcmv share cross-reactive epitopes between nps.The total seroprevalence of wenv in healthy adults was 4.6%, with 3.6% (8/221) for individuals 15-44 years of age, 5.4% (17/317) for individuals 45-59 years of age, and 4.1% (4/98) for older adults over 60.The total seroprevalence of wenv in children under age 15 was 1.5%, with 2.9% (1/34) in children aged 2-5 years, and 2.2% in 5-14 years (2/91).The finding suggests that wenv or wenv-like virus may sporadically infect humans of china.

Journal: Biosafety and health (2020)
Publication types: Journal Article
HDAC11 Regulates Glycolysis through the LKB1/AMPK Signaling Pathway to Maintain Hepatocellular Carcinoma Stemness.

Hepatocellular carcinoma (hcc) contains a subset of cancer stem cells (csc) that cause tumor recurrence, metastasis, and chemical resistance.Histone deacetylase 11 (hdac11) mediates diverse immune functions and metabolism, yet little is known about its role in hcc cscs.In this study, we report that hdac11 is highly expressed in hcc and is closely related to disease prognosis.Depletion of hdac11 in a conditional knockout mouse model reduced hepatocellular tumorigenesis and prolonged survival.Loss of hdac11 increased transcription of lkb1 by promoting histone acetylation in its promoter region, thereby activating the ampk signaling pathway and inhibiting the glycolysis pathway, which in turn leads to the suppression of cancer stemness and hcc progression.Furthermore, hdac11 overexpression reduced hcc sensitivity to sorafenib.Collectively, these data propose hdac11 as a new target for combination therapy in patients with kinase-resistant hcc.Significance: this study finds that hdac11 suppresses lkb1 expression in hcc to promote cancer stemness, progression, and sorafenib resistance, suggesting the potential of targeting hdac11 to treat hcc and overcome kinase inhibitor resistance.

Journal: Cancer research (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Prevalence of Chlamydia trachomatis in Women Who Are Candidates for In Vitro Fertilization in a Private Reference Service in Southern Brazil: A Cross-Sectional Study.

Introduction chlamydia trachomatis(ct) has been related to fallopian tube damage and infertility.Its prevalence in the population that attend public services is known; however, there is scant data on this factor in private infertility clinics.The objective of this study is to verify the prevalence of ct among women attending a private in vitro fertilization (ivf) reference clinic in southern brazil.Methods this is a cross-sectional study carried out between january 1, 2019, and august 30, 2021, at an ivf private clinic in southern brazil.Infertile women between 18 and 50 years old, who provided a morning urinary sample for reverse transcription-polymerase chain reaction(rt-pcr) test for ct analysis, were included in the study.The variables studied included the patient's age, body mass index (bmi), duration of infertility, type of infertility, indication for ivf, and detection or not of ct in the urine.Results the prevalence of ct was 10.84% (22 out of 203; 95% ci: 7.27-15.87).Patients with secondary infertility were older and had more ovarian and tubal factors compared to cases of primary infertility.The tubal factor was the most prevalent (27.3% in women with primary infertility and 35.8% in those with secondary).Time of infertility and bmi were similar between groups.Our results are derived from a single private ivf clinic which reduces the external validity.Conclusion the prevalence of 10.84% of ct in this population raises the importance of screening for sexually transmitted infections for proper treatment and to achieve better ivf outcomes.

Journal: Cureus (2022)
Publication types: Journal Article
Circulating Monocyte Subsets and Transcatheter Aortic Valve Replacement.

Transcatheter aortic valve replacement (tavr), as an alternative to open heart surgery, has revolutionized the treatment of severe aortic valve stenosis (avs), the most common valvular disorder in the elderly.Avs is now considered a form of atherosclerosis and, like the latter, partly of inflammatory origin.Patients with high-grade avs have a highly disturbed blood flow associated with high levels of shear stress.The immediate reopening of the valve during tavr leads to a sudden restoration of a normal blood flow hemodynamic.Despite its good prognosis for patients, tavr remains associated with bleeding or thrombotic postprocedural complications, involving mechanisms that are still poorly understood.Many studies report the close link between blood coagulation and inflammation, termed thromboinflammation, including monocytes as a major actor.The tavr procedure represents a unique opportunity to study the influence of shear stress on human monocytes, key mediators of inflammation and hemostasis processes.The purpose of this study was to conduct a review of the literature to provide a comprehensive overview of the impact of tavr on monocyte phenotype and subset repartition and the association of these parameters with the clinical outcomes of patients with severe avs who underwent tavr.

Journal: International journal of molecular sciences (2022)
Publication types: Journal Article;Review
A lithium-doped surface inspires immunomodulatory functions for enhanced osteointegration through PI3K/AKT signaling axis regulation.

The response of immune systems is crucial to the success of biomedical implantsin vivoand in particular, orthopedic implants must possess appropriate immunomodulatory functions to allow sufficient osteointegration.In this work, lithium (li) is incorporated into titanium (ti) implants by plasma electrolytic oxidation to realize slow and sustained release of li ions.in vitrocellular behaviors of mice bone marrow derived macrophages (bmdms), including gene expression, cytokine secretion, and surface marker analysis suggest that a low dose of li incorporation could enhance the recruitment of bmdms, restrict pro-inflammatory polarization (m1 phenotype), and promote anti-inflammatory polarization (m2 phenotype).Thein vivoair pouch implantation model is constructed to simulate the microenvironment associated with aseptic loosening and the histology results confirm that a small dose of li could relieve inflammatory reactions surrounding the implants.Moreover, compared to the li-free group, the macrophage-conditioned culture medium (mcm) from li-doped samples is more beneficial for the osteogenic differentiation of the mouse embryo cell line (c3h10t1/2) and angiogenesis of human umbilical vein endothelial cells (huvecs), which is further confirmed by better osteointegration ability in the bone implantation model of li-incorporating ti implants.Furthermore, the molecular mechanism study discloses that osteoimmunomodulatory activity of li-incorporating ti implants is achieved by regulating the cascade molecules in the pi3k/akt signalling pathway.This work reveals that favorable immune-modulated osteogenesis and osseointegration of bone implants can be realized by the incorporation of li which broadens the strategy to develop the next generation of immunomodulatory biomaterials.

Journal: Biomaterials science (2021)
Publication types: Journal Article