syedzayyan/paper_mill_benchmark huggingface dataset:
Interaction between bovine mammary epithelial cells and planktonic or biofilm Staphylococcus aureus: The bacterial lifestyle determines its internalization ability and the pathogen recognition.

The main cause of mastitis, one of the most costly diseases in the dairy industry, is bacterial intramammary infection.Many of these bacteria are biofilm formers.Biofilms have been associated with resistance to antibiotics and to the host immune system.Here, we evaluated different experimental models representing bacterial biofilm lifestyle with the aim to study bacterial invasion into bovine mammary epithelial cells and the interaction of these cells with planktonic or biofilm staphylococcus aureus.Staphylococcus aureus v329, its nonbiofilm-forming mutant and bovine mammary alveolar cells (mac-t) were used.Bacterial invasion was studied using the gentamicin exclusion test, cell viability by trypan blue exclusion technique, tlr2 expression by flow cytometry, il1β/il6 production by elisa and il8/tnfα gene expression by real-time polymerase chain reaction.Biofilm and planktonic s.Aureus showed differences in their invasion ability, with the biofilm mode showing a lower ability.Planktonic s.Aureus reduced mac-t viability after 6 h of co-culture, while biofilms did so at 24 h.Mac-t infected with planktonic bacteria showed increased tlr2 expression.Both lifestyles increased il8 expression and il1β/il6 production but did not modify tnfα expression.Our results demonstrate that the bacterial lifestyle affects the invasion behavior, suggesting that biofilms reduce the bacteria-epithelial cell interaction.Planktonic cultures seem to induce higher cellular activation than biofilms.Further knowledge about the complex host-biofilm interaction is necessary to design more efficient therapies against bovine mastitis.

Journal: Microbial pathogenesis (2021)
Publication types: Journal Article
Comparing the outcomes and effectiveness of robotic-assisted sacrocolpopexy and laparoscopic sacrocolpopexy in the treatment of pelvic organ prolapse.

Introduction and hypothesis abdominal sacrocolpopexy is regarded as the gold standard for management of pelvic organ prolapse (pop).Nowadays, minimally invasive surgeries are preferred, and sacrocolpopexy can be performed using either a laparoscopic or robotic-assisted approach.The aim of the current study was to compare the efficacy and safety of robotic-assisted sacrocolpopexy (rasc) and laparoscopic sacrocolpopexy (lsc) through an updated systematic review and meta-analysis.Methods we performed a systematic literature review of different databases and related references from their inception until july 2020 without language restrictions.All randomized control trials and comparative studies that compared rasc and lsc for the management of pop were included.Results a total of 13 studies including 2115 participants were included for the pooled analysis.The pooled results revealed that rasc was associated with a significantly longer operative time (weighted mean difference, 29.53 min; 95% confidence interval [ci], 12.88 to 46.18 min, p = 0.0005), significantly less estimated blood loss (weighted mean difference, -86.52 ml; 95% ci -130.26 to -42.79 ml, p = 0.0001), significantly fewer overall intraoperative complications (odds ratio [or] 0.6; 95% ci 0.40 to 0.91; p = 0.01) and significantly lower conversion rate (or 0.39; 95% ci 0.19 to 0.82; p = 0.01) compared with lsc.There were no significant differences between the length of hospital stays, overall postoperative complications, postoperative stress incontinence, mesh erosion and effectiveness between the two groups.Conclusion the current study showed comparable efficacy between rasc and lsc.Though rasc was associated with less blood loss and a lower conversion rate, the differences were not clinically significant.The choice of surgical procedure with either rasc or lsc is according to surgeon discretion and patient preferences.

Journal: International urogynecology journal (2022)
Publication types: Journal Article;Meta-Analysis;Systematic Review
Neuroprotective effects of natural compounds on neurotoxin-induced oxidative stress and cell apoptosis.

S overproduction of reactive species, notably reactive oxygen (ros) and nitrogen (rns) species, along with the failure of balancing effects of endogenous antioxidant defenses result in destruction of cellular structures, lipids, proteins, and genetic material, which lead to oxidative stress.Oxidative stress-induced neuronal apoptosis plays a pivotal role in pathogenesis of neurodegeneration.Antioxidants represent one of the medical choice strategies for protecting against this unbalanced oxidation-antioxidation status.Recently, natural compounds with neuroprotective potential that can scavenge free radicals and protect cells from oxidative damage have received extensive attention.Methods in this review, we summarized the detailed research progress on the medicinal plants-derived natural compounds with potential anti-oxidation effects and their molecular mechanisms on modulating the neurotoxin (6-ohda, h2o2, glutamate, aβ)-induced oxidative stress and cell apoptosis.Results the natural compounds that efficacious in modulating reactive species production and mitochondrial function include flavonoids, glucosides, alkaloids, polyphenols, lignans, coumarins, terpenoids, quinones and others.They decreased the neurotoxin-induced oxidative damage and apoptosis by (1) decreasing ros/rns generation, lipid peroxidation, caspase-3 and caspase-9 activities, ldh release, the ratio of bax/bcl-2, ca2+influx and cytochrome c release, (2) elevating mmp, and (3) restoring endogenous antioxidant enzymatic activities (cat, gsh-px, gsr, sod).And they exerted neuroprotective effects against cell damages and apoptosis by modulating the oxidative cascades of different signaling pathways (nrf2/ho-1, nf-κb, mapks, pi3k/akt, gsk-3β) and preventing mitochondria-dependent apoptosis pathways.Discussion the present work reviews the role of oxidative stress in neurodegeneration, highlighting the potential anti-oxidation effects of natural compounds as a promising approach to develop innovative neuroprotective strategy.

Journal: Nutritional neuroscience (2022)
Publication types: Journal Article;Review
HIV fragments detected in Kaposi sarcoma tumor cells in HIV-infected patients.

Kaposi sarcoma (ks) is a malignant vascular neoplasm caused by ks-associated herpesvirus (kshv) infection.Hiv plays a major role in ks pathogenesis.Ks in hiv usually produces more malignant features than classic ks.Despite the close ks-hiv relationship, no study has reported the existence of hiv in ks tissue.We used dd pcr to detect hiv and kshv in hiv+ ks samples and classic ks control.We verified ks cell types through immunohistochemistry and applied hypersensitive in situ hybridization (ish) to detect hiv and kshv in tumor cells.Furthermore, we co-stained samples with ish and immunohistochemistry to identify hiv and kshv in specific cell types.Regarding pathological stages, the ks were nodular (58.3%), plaque (33.3%), and patch (8.3%) tumors.Moreover, dd pcr revealed hiv in 58.3% of the ks samples.Ish revealed positive pol/gag m rna signals in cd34 + tumor cells from hiv + patients (95.8%).Hiv signals were absent in macrophages and other inflammatory cells.Most hiv + ks cells showed scattered reactive particles of hiv and kshv.We demonstrated that hiv could infect cd34 + tumor cells and coexist with kshv in ks, constituting a novel finding.We hypothesized that the direct kshv-hiv interaction at the cellular level contributes to ks oncogenesis.

Journal: Medicine (2022)
Publication types: Journal Article
Management of the patent ductus arteriosus in preterm infants.

Management of the patent ductus arteriosus (pda) is one of the most contentious topics in the care of preterm infants.Pda management can be broadly divided into prophylactic and symptomatic therapy.Prophylaxis with intravenous indomethacin in extremely low birth weight infants may reduce severe intraventricular hemorrhage.Echocardiography should be routinely used to confirm the presence of a pda before considering symptomatic therapy.A symptomatic pda can be managed conservatively, using pharmacotherapy or with procedural closure.Ibuprofen should be considered as the pharmacotherapy of choice for a symptomatic pda.High-dose ibuprofen may be preferable, especially for preterm infants beyond the first 3 to 5 days of age.If pharmacotherapy fails (after two courses) or is contraindicated, procedural closure may be considered for infants with a persistent pda with significant clinical symptoms in addition to echocardiographic signs of a large pda shunt volume and pulmonary over-circulation.

Journal: Paediatrics & child health (2022)
Publication types: Journal Article;Review
Cardiac Autonomic Function and Psychological Characteristics of Heterosexual Female Perpetrators of Intimate Partner Physical Aggression.

Intimate partner violence is predominantly viewed as a social problem of men's violence against women.However, a growing evidence base suggests an equal prevalence rate for male and female perpetrated intimate partner physical aggression.Moreover, female perpetrated intimate partner violence is often assumed to be reactive, yet there is limited evidence to support this notion.In this article, we describe the results of two studies that investigated the prevalence of female perpetrated intimate partner physical aggression, and its correlates in heterosexual female university students.The relationships of personality traits, resting heart rate, and heart rate variability (a correlate of vagal activity) were compared between females who did and did not report having engaged in intimate partner physical aggression.In study 1, we found that 30.9% of participants reported enacting intimate partner physical aggression during the preceding 12 months.This finding suggests that a considerable number of undergraduate females aggress against their intimate partners.Perpetrators, relative to nonperpetrators, scored higher on secondary psychopathic traits.In study 2, female intimate partner violence was shown to be associated with low resting heart rate and high heart rate variability.Perpetrators, relative to nonperpetrators, scored higher on psychopathic traits that index emotional resilience and unempathic tendencies, and reported increased proactive and reactive aggression.This raises the possibility that some incidences of female intimate partner physical aggression represent proactive aggressive acts.These findings also support the frequently found association between low resting heart rate and aggression, but raise the prospect that the reported aggressive acts reflect high heart rate variability and strong parasympathetic nervous system activity.

Journal: Journal of interpersonal violence (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Sourdough bacterial dynamics revealed by metagenomic analysis in Brazil.

This study dealt with the influence of the temperature on the bacterial dynamics of two spontaneously fermented wheat sourdoughs, propagated at 21 ± 1 °c (sd1) and 30 ± 1 °c (sd2), during nine backslopping steps (bs1 to bs9).Proteobacteria was the only phylum found in flour.Escherichia hermannii was predominant, followed by kosakonia cowanii, besides species belonging to the genera pantoea and pseudomonas.After one step of propagation, clostridium and bacillus cereus group became predominant.Lactobacillus curvatus was found at low relative abundance.For the second backslopping step, clostridium was flanked by l.Curvatus and lactobacillus farciminis.From bs4 (6th day) onward, lactic acid bacteria (lab) became predominant.L.Farciminis overcame l.Curvatus and remained dominant until the end of propagations for both sourdoughs.At 21 °c, bacillus, clostridium, pseudomonas, and enterobacteriaceae were gradually inhibited.At the end of propagation, sd1 harbored only lab.Otherwise, the temperature of 30 °c favored the persistence of atypical bacteria in sd2, as pseudomonas and enterobacteriaceae.Therefore, the temperature of 21 °c was more suitable for sourdough propagation in brazil.This study enhanced the knowledge of temperature's influence on microbial assembly and contributed to the elucidation of sourdough microbial communities in brazil.

Journal: Food microbiology (2020)
Publication types: Journal Article
Screening and isolation of microbes from a Mud Community of Ischia Island Thermal Springs: preliminary analysis of a bioactive compound.

Introduction balneotherapy centers of ischia island (italy) offer treatments for different dermatological diseases (psoriasis, acne, atopic dermatitis) and upper respiratory tract infections.In this study, we integrated morphological and molecular approaches to give a focus on isolation and screening of extremophile bacteria from ischia thermal mud for potential antimicrobial applications.Methods samples were collected during 2019 at four sites.Some bacterial strains atcc for antibacterial and antibiofilm activity were tested.After morphological characterization, screening for antagonistic isolates was made.The colonies isolated from thermal mud samples were submitted to molecular characterization.Susceptibility testing by dilution spotting was carried out and antibacterial efficacies of most active isolate were evaluated with a minimal inhibition concentration assay.Biofilm formation, inhibition, eradication were examined.Statistical analyses were carried out utilizing microsoft®excel 2016/xlstat©-pro.Results we isolated a natural compound with antimicrobial and antibiofilm activities.Conclusions the results obtained in this study are discussed in the context of how hydrothermal systems are important environmental source of uncharted antimicrobial and antibiofilm compounds.In conclusion, to the most effective of our knowledge, this work presents the primary report on the preliminary investigation of thermophile microbial diversity and their antimicrobial and antibiofilm activities for future biotechnological interest.

Journal: Journal of preventive medicine and hygiene (2021)
Publication types: Journal Article
Sulfur Nutrition Affects Garlic Bulb Yield and Allicin Concentration.

Improving bulb yield and allicin content of garlic is important in meeting fresh and pharmaceutical market demands.Garlic plants have a high demand for sulfur (s) since allicin contains s atoms.Two experiments were conducted to identify the effect of s application rate on garlic yield and quality.In a field trial assessing six s application rates (0-150 kg s ha-1), cultivar 'glenlarge' produced the greatest bulb weight (~90 g) and allicin content (521 mg bulb-1) with the application of 75 kg s ha-1.In contrast, cultivar 'southern glen' showed no response in bulb weight or allicin.This was likely due to high soil background s concentrations masking treatment effects.Subsequently, a solution culture experiment with cv.'glenlarge' evaluated six s application rates (188 to 1504 mg s plant-1, nominally equivalent to 25-200 kg s ha-1).In solution culture, bulb weight and allicin concentration increased with s rate.Highest bulb weight (~53 g bulb-1) and allicin concentration (~11 mg g-1dw) were recorded at an s application of 1504 mg s plant-1.This is the first report to conclusively demonstrate the effect of s on yield and allicin in garlic grown in solution culture.

Journal: Plants (Basel, Switzerland) (2022)
Publication types: Journal Article
Colorectal cancer patients advocating screening to their siblings: a randomized behavioral intervention.

And aims siblings of colorectal cancer (crc) patients are at increased risk of developing crc, but screening rates remain low.Through a randomized behavioral intervention, this study aimed to determine whether patients can advocate screening to their siblings using a tailored educational package.Methods crc survivors were recruited and randomized into relaying either tailored materials (intervention group) or existing national screening guidelines (control group) to their siblings.Siblings could respond to the study team if they were interested in learning about crc screening.Study outcomes were patient advocacy rates (number of patients who had successfully contacted at least 1 eligible sibling) between groups and the proportion of eligible siblings who responded.Results between may 2017 and march 2021, 219 crc patients were randomized to the intervention (n = 110) and control (n = 109) groups.Patient advocacy rates were high and did not differ significantly between groups.However, only 14.3% of eligible siblings (n = 85) responded to the study team.Siblings of patients from the intervention group were more likely to respond (adjusted odds ratio, 1.8; 95% confidence interval, 1.1-3.0; p < .05).Moreover, after controlling for potential confounders, siblings aged ≥60 years were significantly less likely to respond (adjusted odds ratio, .3; 95% confidence interval, .1-.7; p < .01).Conclusions crc patients are willing advocates of screening, and siblings contacted by patients from the intervention group were also more likely to reach out to the study team.However, overall sibling response rates were low despite advocacy, suggesting that patient-led advocacy should at best be used as an adjunct to other, multipronged crc screening promotion modalities.

Journal: Gastrointestinal endoscopy (2022)
Publication types: Journal Article;Randomized Controlled Trial
2D MoS2nanosheets on 1D anodic TiO2nanotube layers: an efficient co-catalyst for liquid and gas phase photocatalysis.

One-dimensional ti o2nanotube layers with different dimensions were homogeneously decorated with 2d mo s2nanosheets via atomic layer deposition and employed for liquid and gas phase photocatalysis.The 2d mo s2nanosheets revealed a high amount of exposed active edge sites and strongly enhanced the photocatalytic performance of ti o2nanotube layers.

Journal: Nanoscale (2019)
Publication types: Journal Article
Characterization of a Caffeic Acid 8-O-Methyltransferase from Citrus and Its Function in Nobiletin Biosynthesis.

Nobiletin (3',4',5,6,7,8-hexamethoxyflavone) is a polymethoxylated flavonoid specifically accumulated in citrus fruit with numerous beneficial effects to human health.In this study, a novel o-methyltransferase (cit omt2) was isolated from three citrus varieties, ponkan mandarin (citrus reticulata blanco), nou 6 ("king mandarin" × "mukaku-kishu"), and satsuma mandarin (citrus unshiu marc.), and its functions were characterized in vitro.The gene expression results showed that cit omt2was highly expressed in the two nobiletin abundant varieties of ponkan mandarin and nou 6.However, the expression level of cit omt2was low in the flavedo of satsuma mandarin, in which only a small amount of nobiletin was accumulated.Functional analysis suggested that cit omt2 was a caffeic acid 8-o-methyltransferase, and it catalyzed the o-methylation of 7,8-dihydroxyflavone at 8-oh.As the methylation of flavone at 8-oh was required for nobiletin biosynthesis, the results presented in this study suggested that cit omt2was a key gene regulating nobiletin accumulation in citrus fruit.

Journal: Journal of agricultural and food chemistry (2022)
Publication types: Journal Article
NLRP6 is required for cancer-derived exosome-modified macrophage M2 polarization and promotes metastasis in small cell lung cancer.

Metastasis remains the primary cause of small cell lung cancer (sclc)-related deaths.Growing evidence links tumor metastasis with a pre-metastatic microenvironment characterized by an anti-inflammatory response, immunosuppression, and the presence of tumor-derived exosomes.To clarify the relationships among these factors in sclc, we analyzed sclc patient samples as well as a mouse model.Among the infiltrating immune cells, our study focused on the tumor-associated macrophages (tams), that are well-known to promote tumor progression and metastasis.We found that high expression of the alternatively activated (m2) tam marker, cd206+was associated clinically with a poorer prognosis and metastasis state in patients with sclc.Moreover, infiltrating macrophages (mø) were found in the metastatic foci of an sclc mouse model.Additionally, we observed dominant switching to m2 phenotype, accompanied by increased nlrp6 expression.Since tumor-derived exosomes are the key links between the tumor and its immune microenvironment, we further investigated whether sclc-derived exosomes contributed to the mø phenotype switch.Our findings showed for the first time that sclc-derived exosomes induce the m2 switch via the nlrp6/nf-κb pathway, and thus, promote sclc metastasis in vitro and in vivo.Collectively, these results indicate a novel mechanism by which sclc-derived exosomes induce immunosuppression of distant mø to promote systemic metastasis by activating nlrp6.Here, we highlight the close relationship between the tumor-derived exosomes, inflammasomes and immune microenvironment in sclc metastasis.

Journal: Cell death & disease (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Pilot study: Evaluation of potential drug-drug interactions in hospitalized pediatric patients.

Evaluate the type and severity of potential drug-drug interactions and identify risk factors involved, in pediatric patients admitted in a hospital setting.Methods transversal retrospective analytical study was carried out with hospitalized pediatric patients from a hospital in the west of mexico, second and third level.The patients included were ≤18 years old hospitalized in the children wards; those admitted at the emergency room, neonatal intermediate and intensive therapy units were not included.Medical prescriptions were reviewed taking into consideration anthropometric characteristics, diagnosis and number of drugs prescribed to identify potential drug-drug interactions using micromedex 2.0 database.Results 88 patients were included, an average of 4.6 ± 2.8 of drugs were prescribed per patient.37 subjects (42%) presented some degree of potential drug-drug interactions of which 25.5% were major and 27.7% moderate according to the software.Identified risk factors were: age ≥ 4 years (or 1.917; 95% ci 1.081-3.399), bsa ≥ 0.8m2(or 1.825; 95% ci 1.021-3.263), height ≥ 1 m (or 2.556;95% ci 1.322 - 4.941), and number of prescribed medications ≥ 4 (or 2.106;95% ci 1.248 - 3.556).Conclusion some of the interactions found were for the benefit of the patient, but others were considered undesirable because they altered the pharmacokinetics of some of the medications administered.Detecting in time the harmful interactions for a patient may favor the patient's safety.

Journal: Pediatrics and neonatology (2020)
Publication types: Evaluation Study;Journal Article;Research Support, Non-U.S. Gov't