syedzayyan/paper_mill_benchmark huggingface dataset:
One for Everyone: A Study of User Satisfaction Among Health-Care Providers Regarding Extended Use of N95 Masks During the COVID-19 Pandemic.

This study was conducted to assess the feasibility of extended use of n95 masks in our hospital during the coronavirus disease 2019 (covid-19) pandemic.We also studied the use pattern, user satisfaction, and issues faced during extended use of the mask.Methods this cross-sectional study was conducted among health-care providers in a large tertiary care teaching hospital in northern india from april 1 to may 31, 2020.A list was prepared from the institute's register, and participants were chosen by random sampling.The data collected from the physical forms were transferred to excel sheets.Results a total of 1121 responses were received.The most common problem stated with reuse of n95 masks was loss of fit followed by damage to the slings, highlighted by 44.6% and 44.4% of the participants, respectively.A total of 476 (42.5%) participants responded that they would prefer "cup-shaped n95 mask with respirator".The median scores regarding the satisfaction with the quality of masks and their fit was also 4 each.Conclusions it was concluded that the extended use of n95 masks was acceptable, with more than 96% of the participants using these masks.

Journal: Disaster medicine and public health preparedness (2022)
Publication types: Journal Article
Development of VER-50589 analogs as novel Hsp90 inhibitors.

As an important target for tumor therapy, heat shock protein 90 has attracted tremendous attention.Through structure analysis, we rationally designed three analogs of ver-50589 which is a known and potent hsp90 inhibitor.Target inhibitory activity result showed that one compound dubbed as 12-1 exhibited strong inhibitory activity against hsp90 with an ic50value of 9 n m.In tumor cell viability experiment, compound 12-1 robustly repressed the proliferation against six human tumor cells with ic50values all in nanomolar range scoring over ver-50589 and geldanamycin.12-1 was able to induce apoptosis of tumor cells and arrest the tumor cell cycle in g0/g1 phase.Meanwhile, western blot results showed that 12-1 could significantly downregulated the expression of two hsp90 client proteins cdk4 and her2.Finally, molecular dynamic simulation showed that compound 12-1 could fit well with atp binding site on n-terminal of hsp90.

Journal: Bioorganic & medicinal chemistry letters (2023)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
The role of oscillation in ellipsoidal drop impact on a solid surface.

Ellipsoidal shapes of drops can significantly modify the impact dynamics and suppress the rebound by inducing symmetry breaking in the mass and momentum distributions compared to the axisymmetric dynamics of typical drops.However, the previous works have assumed that the drop oscillation at the moment of impact only slightly affects the post-dynamics although the oscillation must be involved in the spreading.Here, we study the impact dynamics of the oscillating ellipsoidal drops on non-wetting surfaces as a function of the ellipticity, oscillation phase, and weber number (we) experimentally and numerically.The spreading dynamics show notable hysteretic features in the maximal spreading diameters at the four regions of the oscillation phase.The hysteresis appears more prominently in prolate drops than in oblate drops and becomes remarkably suppressed at the four phases as we increases.Momentum analysis shows that the phases for shaping the drops spherically can drive higher asymmetry in the horizontal momenta than the other phases for shaping the drops ellipsoidally.The momentum asymmetry in the horizontal axes indicates that the oscillation phase as well as the ellipticity can play an important role in altering the hydrodynamics and reducing the bounce magnitude.

Journal: Journal of colloid and interface science (2022)
Publication types: Journal Article
An integrative analysis of DNA methylation and gene expression to predict lung adenocarcinoma prognosis.

Abnormal dna methylation of gene promoters is an important feature in lung adenocarcinoma (luad).However, the prognostic value of dna methylation remains to be further explored.objectives.we sought to explore dna methylation characteristics and develop a quantifiable criterion related to dna methylation to improve survival prediction for luad patients.methods:illumina human methylation450k array data, level 3 rna-seq data and corresponding clinical information were obtained from tcga.Cox regression analysis and the akaike information criterion were used to construct the best-prognosis methylation signature.Receiver operating characteristic curve analysis was used to validate the prognostic ability of the dna methylation-related feature score.Q pcr was used to measure the transcription levels of the identified genes upon methylation.results:we identified a set of dna methylation features composed of 11 genes (myeov,kcnu1,slc27a6,neurod4,hmgb4,tacr3,gabra5,trpm8,nlrp13,edn3and slc34a1).The feature score, calculated based on dna methylation features, was independent of tumor recurrence and tnm stage in predicting overall survival.Of note, the combination of this feature score and tnm stage provided a better overall survival prediction than either of them individually.The transcription levels of all the hypermethylated genes were significantly increased after demethylation, and the expression levels of 3 hypomethylated proteins were significantly higher in tumor tissues than in normal tissues, as indicated by immunohistochemistry data from the human protein atlas.Our results suggested that these identified genes with prognostic features were regulated by dna methylation of their promoters.conclusion:our studies demonstrated the potential application of dna methylation markers in the prognosis of luad.

Journal: Frontiers in genetics (2022)
Publication types: Journal Article
In vivo potency assay for the screening of bioactive molecules on cartilage formation.

Cartilage regeneration is a priority in medicine for the treatment of osteoarthritis and isolated cartilage defects.Several molecules with potential for cartilage regeneration are under investigation.Unfortunately, in vitro chondrogenesis assays do not always predict the stability of the newly formed cartilage in vivo.Therefore, there is a need for a stringent, quantifiable assay to assess in vivo the capacity of molecules to promote the stable formation of cartilage that is resistant to calcification and endochondral bone formation.We developed an ectopic cartilage formation assay (ecfa) that enables one to assess the capacity of bioactive molecules to support cartilage formation in vivo using cartilage organoids.The ecfa predicted good clinical outcomes when used as a quality control for efficacy of chondrocyte preparations before implantation in patients with cartilage defects.In this assay, articular chondrocytes from human donors or animals are injected either intramuscularly or subcutaneously in nude mice.As early as 2 weeks later, cartilage organoids can be retrieved.The size of the implants and their degree of differentiation can be assessed by histomorphometry, immunostainings of molecular markers and real-time pcr.Mineralization can be assessed by micro-computed tomography or by staining.The effects of molecules on cartilage formation can be tested following the systemic administration of the molecule in mice previously injected with chondrocytes, or after co-injection of chondrocytes with cell lines overexpressing and secreting the protein of interest.Here we describe the ecfa procedure, including steps for harvesting human and bovine articular cartilage, isolating primary chondrocytes, preparing overexpression cell lines, injecting the cells intramuscularly and retrieving the implants.This assay can be performed by technicians and researchers with appropriate animal training within 3 weeks.

Journal: Lab animal (2022)
Publication types: Journal Article;Review;Research Support, Non-U.S. Gov't
Characterization of the Blood-Feeding Patterns of Culex quinquefasciatus (Diptera: Culicidae) in San Bernardino County, California.

West nile virus (wnv) is a zoonotic disease that is endemic in north america and is known to cause a range of symptoms from mild to life threatening in humans.Culex quinquefasciatus is one of the most prominent vectors of wnv in southern california.The goal of this study was to identify which animal species are most fed upon by these mosquitoes in various habitats in the west valley area of san bernardino county, california, and determine the relationship between blood-feeding patterns and wnv activity in the region.Culex quinquefasciatus specimens were collected by west valley mosquito and vector control district during 2011 from 32 different sites.The bloodmeals of 683 individuals (92.4% of those tested) were identified using the mitochondrial gene cytochrome c oxidase 1 (coi).These bloodmeals comprised 29 vertebrate species across four different habitats.Species richness (ranging from 10 to 17) was not significantly different between habitats when rarified to account for sample size.Across habitats, the highest percentage of avian bloodmeals were taken from house sparrows (18.8-39.1%) and house finches (2.6-31.5%).Bloodmeals were identified from five mammalian species, accounting for 5.1-59.2% of bloodmeals by habitat, including humans (0-4.1%).A seasonal shift towards increased mammalian bloodmeal prevalence, specifically for domestic dog and human bloodmeals, was observed in urban habitats.The wnv activity during 2011 in san bernardino county occurred mostly in urban and suburban areas as indicated by minimum infection rate (mir) in culex quinquefasciatus, notable as all human bloodmeals were identified from these two habitats.

Journal: Journal of medical entomology (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
The effect of injection therapy and operation of coccydynia on the schooling and physical activity of adolescent patients.

Pain while sitting is the most common complaint among patients with coccydynia.In young patients this may seriously affect their schooling which usually involves long periods of sitting.We studied if this improved after either injection therapy or surgery.During a seven-year period 32 adolescents with a mean age of 15 (range: 11-17) years were treated in our department because of coccydynia.They were followed up with mailed questionnaires at a minimum of 12 months after treatment and 28 (88%) responded.Three were no longer attending school and were excluded.Of the remainder, 12 received only injection therapy while 13 were operated upon.Mean follow-up time was 36 (14-66) months.Eight reported absence from school during the month before treatment, but only one during the week before review (pã0.01).There was significant improvement in sitting during class and physical exercise at school and when engaging in general sports (pã0.01).We can not exclude that improvements would have occurred spontaneously, but this seems unlikely as symptoms had persisted for more than two years before treatment.We conclude that in juveniles, an additional benefit of treating coccydynia with corticosteroid injection or operative resection is that their ability to attend school and take part in sports improves significantly.

Journal: Orthopedic reviews (2020)
Publication types: Journal Article
Neonatal outcome of small for gestational age infants born at 26-33 weeks' gestation in Chinese neonatal intensive care units.

Rate and outcomes of small for gestational age (sga) infants admitted to chinese neonatal intensive care units (nicu) has been poorly demonstrated.We aimed to describe the rate and outcomes of sga preterm infants in chinese nicu, and to evaluate the association of sga status with neonatal outcomes in different gestational age (ga) and birth weight percentile groups.Methods this cohort study included all infants born at 26-33 weeks' gestation and admitted to 25 tertiary chinese nicus from april 2015 to may 2018.Sga was defined as a birthweight <10th percentile for ga based on the chinese neonatal birth weight curve.Results a total of 24,596 infants were included, and 1,867 (7.6%) infants were sga.Sga infants had significantly higher rates of death or any major morbidity (29.8%vs.20.5%), mortality (7.0%vs.4.1%), bronchopulmonary dysplasia (bpd, 17.6%vs.9.8%), necrotizing enterocolitis (nec, 4.8%vs.3.2%) and sepsis (7.3%vs.4.8%) than non-sga infants.Sga status was independently associated with increased risk of death or any major morbidity [adjusted odds ratio: 2.37 (2.08-2.71)] as well as increased risks of death, bpd, rop, death or bpd, death or rop, nec and sepsis.The increased risks of adverse outcomes for sga infants existed across ga groups.The risks of adverse outcomes were highest among infants with a birthweight <3rd percentile.Conclusions sga contributes significantly to adverse neonatal outcomes.Specific attentions are warranted when caring for sga preterm infants.

Journal: Translational pediatrics (2021)
Publication types: Journal Article
Examining the ecological validity of the prototype willingness model for adolescent and young adult alcohol use.

The present study is the first ecologically valid, daily level test of the prototype willingness model (pwm), a model previously tested with hypothetical scenarios to investigate the social reaction and reasoned pathways toward engaging in health-risk behavior.The purpose of the present study is to examine whether days with elevated alcohol-favorable pwm cognitions are also associated with greater intentions and willingness to drink and increased drinking behavior on that day.Participants included 15-25-year-olds (n= 124; 57.3% female;mage=18.7; sd = 2.87) who were part of an ecological momentary assessment study on drinking cognitions (including willingness, intentions, perceived vulnerability, social norms, prototype favorability) and alcohol use, reported across 3 weeks.Analyses accounted for the multilevel structure of the data and the various outcome distributions.Findings supported and advanced the pwm by using real-time, real-world daily data that captured within-person variation of pwm cognitions across days and showed daily level associations between pwm cognitions and alcohol use and negative consequences among adolescents and young adults, thus supporting the social reaction and reasoned pathways at the daily level.Current results may improve interventions by precisely informing the use of technology to bring interventions to adolescents and young adults in moments when they are at highest risk (i.e., days with higher than usual pmw alcohol cognitions).(psyc info database record (c) 2020 apa, all rights reserved).

Journal: Psychology of addictive behaviors : journal of the Society of Psychologists in Addictive Behaviors (2020)
Publication types: Journal Article
Furaltadone suppresses IgE-mediated allergic response through the inhibition of Lyn/Syk pathway in mast cells.

Mast cells are critical cells that prompt various allergic response-inducing factors, contributing to allergic diseases.While used as an antibiotic for livestock, there is no study on the effect of furaltadone on allergic response.This study investigated the effect of furaltadone on mast cells and passive cutaneous anaphylaxis (pca).Furaltadone inhibited the degranulation of mast cells stimulated by antigen (ic50, ~ 3.9 μm), and also suppressed the production of tumor necrosis factor (tnf)-α and interleukin (il)-4 in a concentration dependent manner.In addition, furaltadone inhibited allergic responses in an acute allergy animal model, pca.Further investigation on the mechanism for these inhibitory effects of furaltadone found that the activities of lyn/syk and syk-dependent downstream proteins such as mitogen-activated protein (map) kinases were inhibited by furaltadone in mast cells.Taken together, this study demonstrates that furaltadone inhibits the activation of mast cells by antigen via the suppression of the lyn/syk pathway and ameliorates allergic responses in vivo.

Journal: European journal of pharmacology (2018)
Publication types: Journal Article
Pembrolizumab-induced autoimmune haemolytic anemia in a patient with chronic lymphocytic leukaemia successfully treated with ibrutinib.

We present a unique case of a patient with a long-standing history of indolent chronic lymphocytic leukaemia (cll) who suddenly developed autoimmune haemolytic anaemia after starting immune checkpoint inhibitor therapy for bladder cancer.He had no clear indication to start cll-directed treatment based on current clinical practice guidelines; however, targeted treatment of cll with ibrutinib proved to be effective in treating the haemolytic anaemia.

Journal: BMJ case reports (2021)
Publication types: Case Reports;Journal Article
Practical considerations for feature assignment in high-speed AFM of live cell membranes.

High speed atomic force microscopy (hs-afm) is, in principle, capable of yielding nanometer level detail about the surface of static structures.However, for highly dynamic samples hs-afm may struggle with the correct feature assignment both within and between frames.Feature assignment in hs-afm is dependent on (i) the intrinsic sampling rate, and (ii) the rate of internal redistribution of the sample.Whilst the first quantity (the sampling rate) is defined by the device parameters, the second quantity is frequently unknown, and is often the desired target of the measurement.This work examines how, even in the absence of gross cell morphological change, the rapid dynamics of living cell membranes, may impose an upper spatial limit to the frame-to-frame assignment of cell micro-topography and other related properties (such as local elasticity) whose motion may be described stochastically.Such a practical maximum may prove useful in the setup of hs-afm experiments involving dynamic surfaces thereby facilitating selection of the most parsimonious relationship between observation size, image pixilation and sampling rates.To assist with performing the described calculations a graphical user interface-based software package called hs-afm ugoku is made freely available.

Journal: Biophysics and physicobiology (2022)
Publication types: Journal Article
Combining 3D-Printing and Electrospinning to Manufacture Biomimetic Heart Valve Leaflets.

Electrospinning has become a widely used technique in cardiovascular tissue engineering as it offers the possibility to create (micro-)fibrous scaffolds with adjustable properties.The aim of this study was to create multilayered scaffolds mimicking the architectural fiber characteristics of human heart valve leaflets using conductive 3d-printed collectors.Models of aortic valve cusps were created using commercial computer-aided design (cad) software.Conductive polylactic acid was used to fabricate 3d-printed leaflet templates.These cusp negatives were integrated into a specifically designed, rotating electrospinning mandrel.Three layers of polyurethane were spun onto the collector, mimicking the fiber orientation of human heart valves.Surface and fiber structure was assessed with a scanning electron microscope (sem).The application of fluorescent dye additionally permitted the microscopic visualization of the multilayered fiber structure.Tensile testing was performed to assess the biomechanical properties of the scaffolds.3d-printing of essential parts for the electrospinning rig was possible in a short time for a low budget.The aortic valve cusps created following this protocol were three-layered, with a fiber diameter of 4.1 ± 1.6 μm.Sem imaging revealed an even distribution of fibers.Fluorescence microscopy revealed individual layers with differently aligned fibers, with each layer precisely reaching the desired fiber configuration.The produced scaffolds showed high tensile strength, especially along the direction of alignment.The printing files for the different collectors are available as supplemental file 1, supplemental file 2, supplemental file 3, supplemental file 4, and supplemental file 5.With a highly specialized setup and workflow protocol, it is possible to mimic tissues with complex fiber structures over multiple layers.Spinning directly on 3d-printed collectors creates considerable flexibility in manufacturing 3d shapes at low production costs.

Journal: Journal of visualized experiments : JoVE (2022)
Publication types: Journal Article;Video-Audio Media;Research Support, Non-U.S. Gov't
Association Between ApoA1 Gene Polymorphisms and Antipsychotic Drug-Induced Dyslipidemia in Schizophrenia.

Dyslipidemia frequently occurs in schizophrenia patients treated with antipsychotic drugs (apds), especially atypical apds.Apolipoprotein a1 (apo a1) plays a key role in lipid metabolism.The aim of this study was to investigate whether apo a1 gene polymorphisms are associated with apd-induced dyslipidemia in schizophrenia patients.Patients and methods a total of 1987 patients with schizophrenia were enrolled in this study.Serum lipid profiles were determined with a biochemistry analyzer.Genotyping for the rs5072 polymorphism of apo a1 was performed with taq man assay.Logistic regression analysis was carried out to evaluate the relationship between apo a1 gene polymorphisms and apd-induced dyslipidemia.The effects of drug classification (typical vs atypical apd) and drug regimen (monotherapy vs combination therapy) on serum lipid levels were also analyzed.Results a significant association was found between rs5072 and triglyceride (tg) levels in the recessive model of the logistic regression analysis (adjusted odds ratio [or]=1.50, 95% confidence interval [ci]: 1.03, 2.17; p<0.05).Tg level was significantly higher in patients treated with combination therapy (1.03 (0.71, 1.51) mmol/l) compared to monotherapy (0.93 (0.67, 1.43) mmol/l) and was also associated with sex.There were significant differences in tg levels among the three genotypes of apo a1 rs5072 (gg, ga, and aa) in the whole study population and in patients treated with atypical apds.Conclusion the apo a1 rs5072 variant is associated with dysregulated tg metabolism in schizophrenia patients treated with apds, which may increase susceptibility to dyslipidemia.

Journal: Neuropsychiatric disease and treatment (2021)
Publication types: Journal Article