syedzayyan/paper_mill_benchmark huggingface dataset:
The Zinc Nutritional Immunity ofEpinephelus coioidesContributes to the Importance ofznuCDuringPseudomonas plecoglossicidaInfection.

Previously, the dual rna-seq was carried out in a pseudomonas plecoglossicida-epinephelus coioidesinfection model to investigate the dynamics of pathogen-host interplayin vivo.Znu c, a member of znu cba zn importer, was found transcriptionally up-regulated during infection.Thus, this study aimed to assess its role during the trade-off for zn between host and p.Plecoglossicida.Icp-ms analysis and fluorescent staining showed that zn was withheld from serum and accumulated in the spleen, with increased zn uptake in the golgi apparatus of macrophages after infection.Additionally, growth assay, macrophage infection and animal infection after gene knockout / silencing revealed thatznu cwas necessary for growth in zn-limiting conditions, colonization, intracellular viability, immune escape and virulence of p.Plecoglossicida.Further analysis with dual rna-seq revealed associations of host's zn nutritional immunity genes with bacterial zn assimilation genes.il6and zip4played key roles in this network, and markedly affectedznu bexpression, intracellular viability and immune escape, as revealed by gene silencing.Moreover, emsa and gfp reporter gene analysis showed that fur sensed changes in fe concentration to regulateznu cbain p.Plecoglossicida.Jointly, these findings suggest a trade-off for zn between host and p.Plecoglossicida, while znu cis important for p.Plecoglossicida zn acquisition.

Journal: Frontiers in immunology (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Hairpin-Contained i-Motif Based Fluorescent Ratiometric Probe for High-Resolution and Sensitive Response of Small pH Variations.

Intracellular p h (p hi) is an important parameter associated with cellular behaviors and pathological conditions.Sensing p hi and monitoring its changes are essential but challenging due to the lack of high-sensitive probes.Herein, a ratiometric fluorescent probe with ultra p h-sensitivity is developed based on hairpin-contained i-motif strand (i-strand, labeled with rhodamine green and bhq2at two termini) and complementary strand (c-strand, labeled with rhodamine red at its 5'-end).At neutral p h, both i-strand and c-strand hybridize into a rigid duplex (i-c), which holds the rhodamine red and the bhq2in close proximity.As a result, the fluorescence emission (f597 nm) of the rhodamine red is strongly suppressed, while the rhodamine green (f542 nm) is in a "signal on" state.However, the slightly acidic p h enforced the i-strand to form an intramolecular i-motif and initiated the dehybridization of i-c duplex, leading to rhodamine red in a "signal on" state and a decreased fluorescence of rhodamine green.The ratio (f542 nm/f597 nm) can be used as a signal for p h sensing.Due to the rational internal hairpin design of i-c duplex probe, almost 70-fold change in the ratio was observed in the physiological p h range (6.50-7.40).This probe possesses efficient stability, fast response, and reversible p h measurement capabilities.Furthermore, intracellular application of the ratiometric probe was demonstrated on the example of smmc-7721 cells.With different recognition elements in engineering of i-motif based platforms, the design might hold great potential to become a versatile strategy for intracellular p h sensing.

Journal: Analytical chemistry (2018)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Current perspective on uniportal and multiportal video-assisted thoracic surgery during lobectomy for lung cancer.

Non-small cell lung cancer (nsclc) is the most typical type of lung cancer, and it is the leading cancer-related mortality globally.Lobectomy for early-stage nsclc has been characterized in the previous decade using a wide range of methodologies.The development of video-assisted thoracoscopic surgery (vats) allowed surgeons first to reduce the thoracotomy size, which is generally anterior, limiting it to trocar incisions or a single portal approach.This review aimed to describe current perspectives on operative outcomes, lymph node removal, oncologic outcomes, and advantages for surgeons performing uniportal vats (u vats) and multiportal (m vats) lobectomy.The advantages of u vats include comfortable operating for surgeons with a direct view and safety, and for patients more favourable operative outcomes.Also, the u vats approach has previously been demonstrated to be effective and safe, with positive outcomes not just with respect to cosmetics but also in terms of a speedy recovery.Oncological u vats clearance is comparable to multiportal vats with respect to early mid-term survivability as well as nodal staging, as per retrospective comparison studies.However, the interpretation of outcomes must be made cautiously due to selection bias as well as lack of long-term follow-up; the choice of which vats approach to utilize for patients' treatment following pulmonary resection is largely based on the preferences of the surgeon.As a result, it is difficult to say if one vats method is better than another.

Journal: Kardiochirurgia i torakochirurgia polska = Polish journal of cardio-thoracic surgery (2022)
Publication types: Journal Article;Review
Association Between Periodontitis and Preterm Birth in a Cohort of Pregnant Women in Ivory Coast.

The aim of this study was to investigate the possible association between periodontitis and preterm birth in ivory coast.Materials and methods a cohort study ﻿﻿including 446 volunteers (pregnant women) aged 15-50 years was performed in the gynecology-obstetrics department of the university hospital center of cocody-abidjan in ivory coast.Socioeconomic and periodontal status was obtained during pregnancy.After delivery, obstetric data was collected.Periodontitis was diagnosed according to the new 2018 efp/aap classification of periodontal and peri-implant diseases and conditions, as follows: a subject presenting with interdental cal at two non-adjacent teeth or buccal/oral cal ≥ 3 mm with pocketing > 3 mm was diagnosed with periodontitis.Any birth before the 37th week was considered a preterm birth (ptb).Results the prevalence of periodontitis and preterm birth were 59.47% and 18.34%, respectively.Periodontitis was mainly stage 1.Ptb was statistically significantly higher in pregnant women with periodontitis compared to women without periodontitis (p = 0.0002).Multivariate analysis showed that periodontitis was associated with ptb (p = 0.0002).Logistic regression showed that periodontitis is a risk factor for preterm birth (or = 3.62; 95% ci: 1.80-7.31; p = 0.0003).Conclusion the results of this study suggest ﻿that periodontitis is an additional risk factor for preterm birth in ivory coast.

Journal: Oral health & preventive dentistry (2022)
Publication types: Journal Article
Selective functionalization of benzylic C-H bonds of two different benzylic ethers by bowl-shapedN-hydroxyimide derivatives as efficient organoradical catalysts.

A highly efficient, site-selective benzylic c-h bond amination of two different benzylic ether substrates was described by using bowl-shaped n-hydroxyimide organoradical catalysts with diethyl azodicarboxylate.The synthetic utility of this approach is demonstrated by the subsequent transformation of the amination products into the corresponding aldehydes and alkylhydrazines.

Journal: Chemical communications (Cambridge, England) (2022)
Publication types: Journal Article
Potential Functional Embedding Theory with an Improved Kohn-Sham Inversion Algorithm.

Potential functional embedding theory (pfet) is a rigorous theory that can yield a unique, self-consistent embedding potential shared by different subsystems treated at different levels of theory.Application of pfet has been limited by the time-consuming and sometimes unstable optimized effective potential (oep) procedure.Here, we improve the performance of pfet by replacing the oep algorithm with a new method to reconstruct the effective kohn-sham (ks) potential.We propose a direct, efficient ks inversion algorithm to solve for the effective ks potential and then employ the resulting algorithm in pfet.We benchmark our ks inversion algorithm against the recently reported modified ryabinkin-kohut-staroverov (m rks) procedure.Numerical examples show that, with sufficiently large basis sets, our ks inversion algorithm generates almost as accurate results as the m rks procedure does, except in the vicinity of atomic nuclei, and that it requires less computational time.Three types of chemical interactions then were tested using the new ks inversion algorithm in pfet; the energetics computed from the updated formalism compare well to benchmarks.

Journal: Journal of chemical theory and computation (2018)
Publication types: Journal Article
Plain Radiographic Analysis of Laryngeal Dimensions in Young Children: Normal versus Croup.

(1) background: contrary to a tenet of the funnel-shaped pediatric larynx with the cricoid level being narrowest, recent studies show the glottis and subglottis as the narrowest levels.To locate the functionally narrowest level of the larynx, we reported normal laryngeal dimensions and their croup-related changes in young children.(2) methods: we reviewed normal plain neck radiographs recorded for the evaluation of minor trauma or foreign bodies in 504 children aged ≤4 years who visited the emergency department from 2016 through 2021.Using computed tomography-based localization of the glottis, we radiographically defined the subglottis and cricoid.At these levels, we measured diameters and calculated cross-sectional areas (csas) on the radiographs.The values were compared to the equivalent values of a 1:1 age-matched population with croup.(3) results: in the study population (n = 401), the narrowest diameter and csa were observed in the glottis.In detail, the mean anteroposterior/transverse diameters were 9.8/3.4 mm at the glottis, 8.5/5.6 mm at the subglottis, and 7.4/6.8 mm at the cricoid (p < 0.001), respectively.In the same order, the mean csas were 26.5, 38.1, and 40.5 mm2 (p < 0.001).All dimensions were narrower in the croup population (p < 0.001).We found croup-related narrowing, namely reductions in the transverse diameter and csa that were more severe closer to the glottis (p < 0.001), without differences per level in the anteroposterior diameter.(4) conclusions: this study confirms the glottis as the narrowest level of the larynx in young children.In addition, level-based differences in croup-related narrowing suggest some point between the glottis and subglottis as the functionally narrowest level.

Journal: Children (Basel, Switzerland) (2022)
Publication types: Journal Article
Recycled paints: Are they as variable as they seem?

Several paint companies recently included a range of recycled paints among their proposed products.These are produced from recovered waste and mixed according to their main binders and colors to produce new batches of lower quality at lower cost.As they are relatively new to the market, no comparative study of their differentiation has yet been realized.We therefore collected different production batches of recycled paints of 7 different colors (white, off-white, blue, green, yellow, gray and brown).The paints were analyzed optically, by microscopy, infrared and raman spectroscopy and pyrolysis gc/ms.The intended goal was to verify if the common knowledge about paint frequencies and discriminating power of analytical techniques still apply to recycled paints.We have observed that microscopy (presence of colored spots) and pyrolysis gc/ms (high discriminating power) were the recommended techniques to discriminate between production batches.These differences were less pronounced in infrared and raman analysis, which on the other hand represent an average spectrum of the most frequent compounds mixed together.While the composition of the main components is statistically stable and reflects the most common binders and extenders used in paint within a population, the minor components are much more variable and provide an almost unique signature to each of the recycled paints, better recognized by pyrolysis gc/ms than infrared and raman spectroscopies.

Journal: Forensic science international (2022)
Publication types: Journal Article
Comparison of 4-factor PCC reversal of apixaban and rivaroxaban versus warfarin for intracranial hemorrhage.

The purpose of this study is to assess efficacy of 4-factor prothrombin complex concentrates (4f-pcc) for direct oral anticoagulant (doac)-associated intracranial hemorrhage (ich) as compared to its use in warfarin-associated ich.A retrospective cohort study was performed to compare the efficacy of 4f-pcc for reversal of apixaban and rivaroxaban versus warfarin for ich at cooper university health care from january 2015 to december 2019.Patients included were ≥ 18 years of age who developed an ich while on apixaban, rivaroxaban, or warfarin.The primary outcome was to compare the percentage of patients with excellent or good hemostatic efficacy after 4f-pcc administration.Secondary outcomes were to describe functional outcomes at discharge, in-hospital mortality, and thrombotic complications after 4f-pcc administration.A total of 159 patients were included; 115 patients received warfarin and 44 patients received a doac (apixaban, n = 22; rivaroxaban, n = 22).70 patients were evaluable for the primary endpoint.Thirty-four (66.7%) patients in the warfarin group versus 14 (73.7%) patients in the doac group were determined to have excellent or good hemostatic efficacy (p = 0.57).In-hospital mortality (30.4% vs.40.9%, p = 0.21) and thrombotic complications (9.6% vs.11.4%, p = 0.67) were comparable between the warfarin vs.Doac groups, respectively.This small, retrospective study found no difference in patients with excellent/good hemostatic efficacy after reversal with 4f-pcc for doac-associated ich compared to warfarin-associated ich.This study is limited by its retrospective nature and sample size.Larger, prospective studies are needed to further determine the efficacy of 4f-pcc in reversing doac-associated ich.

Journal: Journal of thrombosis and thrombolysis (2022)
Publication types: Journal Article
Ferryl Hemoglobin Inhibits Osteoclastic Differentiation of Macrophages in Hemorrhaged Atherosclerotic Plaques.

Intraplaque hemorrhage frequently occurs in atherosclerotic plaques resulting in cell-free hemoglobin, which is oxidized to ferryl hemoglobin (fhb) in the highly oxidative environment.Osteoclast-like cells (olcs) derived from macrophages signify a counterbalance mechanism for calcium deposition in atherosclerosis.Our aim was to investigate whether oxidized hemoglobin alters osteoclast formation, thereby affecting calcium removal from mineralized atherosclerotic lesions.Rankl- (receptor activator of nuclear factor kappa-βligand-) induced osteoclastogenic differentiation and osteoclast activity of raw264.7 cells were studied in response to oxidized hemoglobin via assessing bone resorption activity, expression of osteoclast-specific genes, and the activation of signalization pathways.Olcs in diseased human carotid arteries were assessed by immunohistochemistry.Fhb, but not ferrohemoglobin, decreased bone resorption activity and inhibited osteoclast-specific gene expression (tartrate-resistant acid phosphatase, calcitonin receptor, and dendritic cell-specific transmembrane protein) induced by rankl.In addition, fhb inhibited osteoclastogenic signaling pathways downstream of rank (receptor activator of nuclear factor kappa-β).It prevented the induction of traf6 (tumor necrosis factor (tnf) receptor-associated factor 6) and c-fos, phosphorylation of p-38 and jnk (c-jun n-terminal kinase), and nuclear translocation of nfκb (nuclear factor kappa-β) and nfatc1 (nuclear factor of activated t-cells, cytoplasmic 1).These effects were independent of heme oxygenase-1 demonstrated by knocking down ho-1 gene in raw264.7 cells and in mice.Importantly, fhb competed with rank for rankl binding suggesting possible mechanisms by which fhb impairs osteoclastic differentiation.In diseased human carotid arteries, olcs were abundantly present in calcified plaques and colocalized with regions of calcium deposition, while the number of these cells were lower in hemorrhagic lesions exhibiting accumulation of fhb despite calcium deposition.We conclude that fhb inhibits rankl-induced osteoclastic differentiation of macrophages and suggest that accumulation of fhb in a calcified area of atherosclerotic lesion with hemorrhage retards the formation of olcs potentially impairing calcium resorption.

Journal: Oxidative medicine and cellular longevity (2020)
Publication types: Journal Article
An Ethical Framework for the Use of Horses in Competitive Sport: Theory and Function.

Growing ethical concern about equestrian sport is reflected in publications by regulatory authorities, animal charities, and the lay press; and in government debate and social media.However, attempts by regulators and stakeholders to address ethical issues in equine sport have been discipline specific and ad hoc.Ethical frameworks can help stakeholders to make contextual decisions about what should or should not be done in a particular situation.However, when existing animal welfare frameworks and existing sports ethics frameworks are reviewed in this paper, it becomes clear that none provide us with a suitable or sufficient tool for considering ethical issues which can arise in situations where the athlete is a non-human, non-consenting participant.This paper presents the theoretical development of a novel ethical framework, with the aim of providing stakeholders with a tool which they might apply to the consideration of the ethical questions which inevitably arise in relation to (equestrian) sport.The derivation and limitations of the ethical framework are explained.The use of the framework will serve both to underwrite the continuation of the social license to use horses in sport and also to enable those within equestrian sport to critically assess existing and proposed practices and to make welfare-improving adjustments to practice if/where necessary.The theoretical framework as presented here is currently being practically tested and refined in consultation with industry stakeholders, and that research will be submitted for publication in due course.

Journal: Animals : an open access journal from MDPI (2021)
Publication types: Journal Article
Benign Parathyroid Hyperplasia With Incidental Pulmonary Seeding on 99mTc-MIBI Scintigraphy and FDG PET/CT.

A 50-year-old woman with end-stage renal disease presented with recurrent hyperparathyroidism after parathyroidectomy.99m tc-mibi scintigraphy did not show mibi-avid lesion in the neck or mediastinum but in bilateral lung fields instead.On suspicion of malignancy, 18f-fdg pet/ct was performed and depicted no significant fdg uptake throughout the whole body.After resection of the pulmonary nodules, the final histopathology revealed benign parathyroid hyperplasia with pulmonary seeding, which is exceptionally a rare entity.

Journal: Clinical nuclear medicine (2022)
Publication types: Case Reports;Journal Article
Sociodemographic Factors Associated with Adolescents' and Young Adults' Susceptibility, Use, and Intended Future Use of Different E-Cigarette Devices.

Numerous studies have identified sociodemographic factors associated with susceptibility, ever-use and past-30-day use of e-cigarettes, including juul.However, it remains unknown which sociodemographic factors are associated with adolescents' and young adults' (aya) use of the entire spectrum of different types of e-cigarette devices (e.g., disposables, pod/cartridge-based, and other e-cigarettes, like mods or tanks).The aim of this study was to examine the relationship between sociodemographic factors and use, future use intent and susceptibility to use different e-cigarette device types.We conducted a national online survey using a convenience sample of 13-24-year-olds, 50:50 e-cigarette ever- to never-users and sex and race/ethnicity balanced per the u.s.Census (n = 4351).Sociodemographic factors were not associated with ever use of disposables among ayas or generally with intent to use e-cigarette devices in the future.However, sociodemographic factors were related to the use of pod/cartridge-based and other e-cigarette devices.Lgbtq+ ayas were more likely to use pod/cartridge-based devices and to be susceptible to using all device types compared to other ayas.Young adults, males, and other/multiracial non-hispanic ayas were more likely to report past-30-day-use of all devices and aa/black non-hispanic ayas were more likely to report past-30-day use of pod/cartridge-based and other devices compared to former users.Aa/black non-hispanic ayas were more likely to be susceptible to using all devices and other/multiracial non-hispanic ayas were susceptible to using other devices (compared to white non-hispanic ayas).Ayas under 21 who were former users were more likely to intend using other devices in the future compared to ayas 21 years or above.These findings may inform targeted prevention efforts to curb the growing popularity of different devices among ayas.

Journal: International journal of environmental research and public health (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural;Research Support, Non-U.S. Gov't
Characteristics in Initial Sandplay of Optimal and Non-optimal Family Functioning Among High School Students.

The purpose of this study is to explore the characteristics of the initial sandplay of high school students with poor family function, by studying the initial sandplay of high school students with different levels of family function, so as to provide the basics for the psychological assessment and intervention of high school students.Significance to provide data support for high school students' mental health education and sandplay therapy, and to apply sandplay as an intervention method to play a healing role in high school mental health work.Methods high school students (n= 345) were divided into 11 groups and participated in a sandplay experiment for 8 weeks from february to march 2021.Each group completed the chinese version of the epstein et al.Family assessment device.Samples with scores of one standard deviation below and above the sample mean were placed in the low family functioning group (n= 30; 15 girls) or high family functioning group (n= 30; 15 girls), respectively.The initial sandplay was evaluated on multiple dimensions using an established coding system.Results samples with low family functioning used fewer animal figurines in their sandplay (t= 2.176,p< 0.05); had more family scenes (χ2= 4.356,p< 0.05); fewer rural scenes (χ2= 4.344,p< 0.05); and fewer healing themes (t= -2.336,p< 0.05).In particular, there were fewer examples of connected healing themes (χ2= 7.500,p< 0.05); in-depth healing themes (χ2= 5.455,p< 0.05); and more hindered trauma themes (χ2= 4.812,p< 0.05).Logistic regression analysis was conducted using the sandplay characteristics as predictors of group membership.Using forward stepwise selection, themes of hindered trauma (b= -2.030) and connected healing (b=1.765) were shown to be significant predictors of group membership (f= 17.784,p< 0.01, δr2= 0.214).Conclusion students who reported high and low family functioning showed significant differences in their sandplay characteristics.We propose sandplay characteristics can help identify low family functioning of adolescents during psychological evaluations.

Journal: Frontiers in psychology (2022)
Publication types: Journal Article