syedzayyan/paper_mill_benchmark huggingface dataset:
Anti-atopic dermatitis effect of a modified Huang-Lian-Jie-Du decoction and its active fraction on 2,4-dinitrobenzene and MC903-induced mouse models.

Huang-lian-jie-du decoction is a traditional chinese medicine formula which has long been used to treat inflammatory skin disease including ad.However, gardeniae fructus, a component herb of hljdd, has noticeable toxicity in liver and kidney.We therefore replaced gardeniae fructus with dictamni cortex with a hope to derive at a modified hljdd (mhljdd) with better safety profile.Purpose the present study aimed to develop mhljdd and identify its active fraction as innovative therapeutic agents for ad using 2,4-dinitrobenzene (dncb) and calcipotriol (mc903)-sensitized mouse models of ad.Methods mhljdd and the combination of the 1-butanol-soluble-fraction and the water-soluble-fraction (mhljdd-f) were given intragastrically to the dncb-induced mice and mc903-induced mice for two weeks.The body weight, dorsal skin/ear thickness and severity of ad symptoms of the mice were measured throughout the study.Scratching behaviors were observed after drug treatment.The blood and dorsal skin/ear tissues of mice were harvested for histopathological examination and biochemical analyses.Results the results revealed that dncb- and mc903-induced ad symptoms, including skin thickening, dryness, erythema and excoriations, in the dorsal skin and ears were significantly alleviated in the mhljdd and mhljdd-f-treated mice.Ceramides content and protein expressions of filaggrin and loricrin were also up-regulated after treatment with mhljdd and mhljdd-f.In addition, skin inflammation induced by dncb and mc903 were markedly suppressed in the mhljdd and mhljdd-f-treated mice, and the action mechanisms involve suppression of the release of inflammatory cytokines, as well as downregulation of the activation of nf-κb and mapks pathways.Besides, mhljdd and mhljdd-f could reverse the abundance of gut microbiota induced by dncb in mice.Conclusions mhljdd and mhljdd-f could markedly relieve ad-like symptoms induced by dncb and mc903 in mice through, at least in part, improving the epidermal barrier function and inhibiting skin inflammation via suppressing the activation of nf-κb and mapks pathways and regulation of the gut microflora dysbiosis.This study reported for the first time that mhljdd and its active fraction could be used as innovative therapeutic agents for ad.

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (2022)
Publication types: Journal Article
Psychometric properties of the Dynamic Risk Outcome Scales (DROS) for individuals with mild intellectual disability or borderline intellectual functioning and externalizing behaviour problems.

The present study examined the psychometric properties of the dynamic risk outcome scales (dros), an instrument developed to measure dynamic risk factors in individuals with mild intellectual disabilities or borderline intellectual functioning (mid-bif) and externalizing (including offending-like) behaviour problems.Method the sample consisted of 606 clients (86% male) from inpatient treatment wards at a facility for individuals with mid-bif and externalizing behaviour problems.Results the dros showed an acceptable factor structure, good internal consistency, significant test-retest reliability and fair to excellent inter-rater reliabilities for most subscales and total score.Compared to the adult behavior checklist (abcl; achenbach & rescorla, 2003 manual for the aseba adult forms and profiles.Burlington, vt: university of vermont, research center for children, youth, & families), the dros showed convergent and divergent validity, concurrent and longitudinal validity.Conclusions the dros is a reliable and valid instrument to measure dynamic risk factors in clients with mid-bif.Future research on the dros will focus on the assessment of recidivism and the inclusion of internalizing problems.

Journal: Journal of applied research in intellectual disabilities : JARID (2020)
Publication types: Journal Article
Printability during projection-based 3D bioprinting.

Since projection-based 3d bioprinting (pbp) could provide high resolution, it is well suited for printing delicate structures for tissue regeneration.However, the low crosslinking density and low photo-crosslinking rate of photocurable bioink make it difficult to print fine structures.Currently, an in-depth understanding of the is lacking.Here, a research framework is established for the analysis of printability during pbp.The gelatin methacryloyl (gel ma)-based bioink is used as an example, and the printability is systematically investigated.We analyze the photo-crosslinking reactions during the pbp process and summarize the specific requirements of bioinks for pbp.Two standard quantized models are established to evaluate 2d and 3d printing errors.Finally, the better strategies for bioprinting five typical structures, including solid organs, vascular structures, nerve conduits, thin-wall scaffolds, and micro needles, are presented.

Journal: Bioactive materials (2022)
Publication types: Journal Article
Nano differential scanning fluorimetry for comparability studies of therapeutic proteins.

Differential scanning calorimetry (dsc) has been extensively used in the biopharmaceutical industry to characterize protein thermal stability and domain folding integrity.Recently, nano differential scanning fluorimetry (nano dsf) has emerged as a powerful tool for thermal stability analysis and studies of protein domain unfolding.Due to increased interests in the qualification of characterization methods, we are in this study presenting the qualification results for the comparability studies of thermal stability analysis using nano dsf.The results show that nano dsf is able to detect thermal transition signals for m abs, bi te® molecules, and cytokines at a wide concentration range with high precision, clearly indicating that nano dsf is suitable for characterization including comparability studies of therapeutic proteins.Compared to the current recognized industry standard dsc, the nano dsf method enables thermal stability analysis over a much wider concentration range, consumes considerably less materials, and provides significantly higher throughput.

Journal: Analytical biochemistry (2020)
Publication types: Journal Article
Past, Present and Future: The Relationship Between Circular RNA and Immunity.

The immune system has evolved since the birth of humans.However, immune-related diseases have not yet been overcome due to the lack of expected indicators and targeting specificity of current medical technology, subjecting patients to very uncomfortable physical and mental experiences and high medical costs.Therefore, the requirements for treatments with higher specificity and indicative ability are raised.Fortunately, the discovery of and continuous research investigating circular rnas (circ rnas) represent a promising method among numerous methods.Although circ rnas wear regarded as metabolic wastes when discovered, as a type of noncoding rna (nc rna) with a ring structure and wide distribution range in the human body, circ rnas shine brilliantly in medical research by virtue of their special nature and structure-determined functions, such as high stability, wide distribution, high detection sensitivity, acceptable reproducibility and individual differences.Based on research investigating the role of circ rnas in immunity, we systematically discuss the hotspots of the roles of circ rnas in immune-related diseases, including expression profile analyses, potential biomarker research, nc rna axis/network construction, impacts on phenotypes, therapeutic target seeking, maintenance of nucleic acid stability and protein binding research.In addition, we summarize the current situation of and problems associated with circ rnas in immune research, highlight the applications and prospects of circ rnas in the treatment of immune-related diseases, and provide new insight into future directions and new strategies for laboratory research and clinical applications.

Journal: Frontiers in immunology (2022)
Publication types: Journal Article;Review;Research Support, Non-U.S. Gov't
Tolerability of Concurrent Chemoradiation Therapy With Gemcitabine (GemX), With and Without Prior Neoadjuvant Chemotherapy, in Muscle Invasive Bladder Cancer.

The aim of this study is to assess the tolerability of concurrent chemoradiation therapy with gemcitabine (gem x) in muscle invasive bladder cancer following neoadjuvant chemotherapy (neo gem x) by use of patient- and provider-reported outcomes.Methods and materials seventy-eight patients were treated with gem x.Thirty-eight received prior neoadjuvant chemotherapy (nac).Patients were prospectively assessed during treatment and at 6 weeks and 12 months after treatment completion.Radiation therapy was given to a total dose of 52.5 gy in 20 fractions with weekly concurrent gemcitabine chemotherapy, 100 mg/m2.Toxicity was assessed by the care provider and by a patient-reported outcome questionnaire collecting scores on the late effects in normal tissues-subjective, objective, management, and analytic scales and was statistically compared at baseline and 12 months, as well as between the neo gem x and gem x groups.Results the median duration of follow-up was 15.9 months.The radiation therapy completion rate was 95%, and 96% of patients completed at least 3 cycles of gemcitabine.Bowel toxicity of grade 3 or greater was reported in 7 of 38 patients (18%) in the neo gem x group and 5 of 25 (20%) in the gem x group.Three gem x and two neo gem x patients had grade 3 or greater urinary toxicity.Forty-nine patients completed questionnaires and were included in the analysis.Scores on the late effects in normal tissues-subjective, objective, management, and analytic scales showed an expected peak by week 4 of treatment.There was no statistically significant difference between mean scores at baseline and 12 months after treatment completion or between the neo gem x and gem x groups.Conclusions this study demonstrates that gem x, alone or following nac, has manageable toxicity and acceptable treatment completion rates.Allowing for small patient numbers and the nonrandomized nature of this study, these results do not suggest any additional toxicity from the use of nac prior to gem x.

Journal: International journal of radiation oncology, biology, physics (2017)
Publication types: Controlled Clinical Trial;Journal Article
Association of PD-1/PD-L1 expression and Epstein--Barr virus infection in patients with invasive breast cancer.

Causative factors of breast cancer include infections, such as epstein-barr virus (ebv) infection.The aim of this study was to analyze the clinicopathological features of ebv-positive (ibc) and determine if ebv affects programmed cell death receptor 1 (pd-1)/pd ligand 1 (pd-l1) expression in ibc, similar to other ebv-infected tumors with pd-l1/pd-1 expression.Methods we collected 140 samples of ibc tissues and 25 samples of adjacent tissues.All patients were followed-up by telephone from the day of surgery to december 2020.Chromogenic in-situ hybridization was performed to evaluate ebv-encoded rna (eber).Immunohistochemistry was performed to evaluate pd-l1 and pd-1 expressions.The correlation between pd1/pdl1 expression and clinicopathological features was also analyzed.Results eber was detected in 57 of 140 (40.7%) ibc tissues and not detected in any adjacent tissue (p 0.05).The positive rate of pd-1/pd-l1 expression was higher in the ebv-positive group than in the ebv-negative group (p < 0.05).The kaplan-meier univariate survival analysis showed that ebv was associated with poor disease-free survival and overall survival in patients with ibc.Pd-l1/pd-1 expression could predict a poor prognosis.Conclusions in this study, clinicopathologic characteristics of patients were consistent with ebv-infected ibc.Patients with ebv-positive breast cancer were more likely to have elevated pd-1/pdl-1 expression compared to those with ebv-negative breast cancer.This finding could serve as a basis to explore therapeutic targets, particularly immunotherapy, for patients with ibc.

Journal: Diagnostic pathology (2022)
Publication types: Journal Article
A highly polymorphic caprine keratin-associated protein gene identified and its effect on cashmere traits.

Five keratin-associated protein 6 genes (krtap6) have been identified in sheep and variation in some krtap6 has been associated with wool fiber diameter-related traits, but none of these homologues have been identified in goats.In this study, we reported the identification of the sheep krtap6-5 homologue on goat chromosome 1 and polymerase chain reaction (pcr)-single strand conformation polymorphism analysis in 300 longdong cashmere goats revealed the existence of 12 variant sequences.Both coding region and 3'utr of the putative caprine krtap6-5 displayed a biggest sequence similarity to ovine krtap6-5 gene.This suggested that the gene represents caprine krtap6-5 sequences, and these sequences composed 23 genotypes, which was the most polymorphism gene in krtaps that have been studied.Among these sequences, 15 nucleotide substitutions and a 24-bp insertion/detection were identified.Variation in goat krtap6-5 was associated with variation in mean-fiber diameter, suggesting that krtap6-5 is worthy of further study in the context of variation in cashmere traits.

Journal: Journal of animal science (2021)
Publication types: Journal Article
Efficacy of pregabalin for the treatment of chronic pruritus of unknown origin, assessed based on electric current perception threshold.

Chronic pruritus of unknown origin (cpuo) is defined as itching lasting more than 6 weeks in the absence of discernible skin lesions.Pregabalin is used to treat patients with cpuo.In this study, we aimed to investigate differences in the perception threshold of itch sensation between patients with cpuo and healthy individuals and to evaluate the efficacy of pregabalin for cpuo.At baseline, week 2, and week 4 after treatment initiation, the visual analogue scale (vas) score was measured to assess pruritus severity, and electric current perception threshold (cpt) was measured at 250 and 5 hz using a neurometer cpt/c stimulator.Twenty healthy individuals and 41 patients with cpuo were enrolled in this study.The patients with cpuo were categorised as those who responded to antihistamines (antihistamine group), were not improved by antihistamines (pregabalin group), and were not improved by antihistamines and pregabalin (refractory group).The baseline cpt values were not significantly different between patients with cpuo and healthy control.Pruritus was improved in 7 of 10 patients in the pregabalin group after treatment with pregabalin, showing decreased cpt at 5 hz.The sensitive c-fibres presented a high threshold to detect itch sensation, and this sensitivity decreased in response to treatment with pregabalin.

Journal: Scientific reports (2020)
Publication types: Journal Article
Fatty acid balance regulates α-synuclein pathology.

A recent study by tripathi et al.Used a protein engineering approach to demonstrate that cellular stress caused by familial α-synuclein mutations can be alleviated by altering the monounsaturated fatty acid equilibrium in neuronal cells.This work supports the notion that metabolic perturbation of lipids may be involved in the pathogenesis of parkinson's disease.

Journal: Trends in neurosciences (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't;Comment
Polysorbate-80 pretreatment contributing to volatile fatty acids production associated microbial interactions via acidogenic fermentation of waste activated sludge.

Polyoxyethylene dehydration sorbitol monooleate (polysorbate-80) pretreatment enhanced volatile fatty acids (vfas) production of waste activated sludge (was) in acidogenic fermentation.The results showed that polysorbate-80 ameliorated was solubilization obviously with a soluble chemical oxygen demand (scod) increasing to 1536 mg/l within 4 h.Within 2 days of acidogenic fermentation, the maximal vfas arrived to 2958.35 mg cod/l via polysorbate-80-pretreatment.The polysorbate-80 pretreatment boosted microbial diversity and richness in fermentation process.The clostridium, macellibacteroides and acidocella strengthened microbial cooperation for the metabolic functions enhancement (e.g.Amino acid metabolism and carbohydrate metabolism) for vfas generation from was organics.Overall, the polysorbate-80 could play positive roles on the transformation of organic matter from sludge solid matters to vfas, which was turned out to become an effective enhancing strategy for future was treatment / bioresource recovery with relatively low cost.

Journal: Bioresource technology (2022)
Publication types: Journal Article
Sex-specific effects of age and body mass index on antibody responses to seasonal influenza vaccines in healthcare workers.

Healthcare institutions with mandatory influenza vaccination policies have over 90% vaccination rates among healthcare workers (hcws) resulting in a population that has received the influenza vaccine in many, consecutive years.This study explored the impact of sex and other host factors in pre- and post-vaccination neutralizing antibody (n ab) titers and seroconversion against the h1n1 and h3n2 influenza a viruses (iavs) among hcws enrolled into a cross-sectional serosurvey during the annual johns hopkins hospital employee vaccination campaign in the 2017-18 and 2018-19 seasons.The study enrolled 111 participants (male = 38, female = 73) in 2017-18 and 163 (male = 44, female = 119) in 2018-19.Serum samples were collected immediately prior to vaccination and approximately 28 days later and n ab titers to vaccine strains determined.An intersectional approach was used to disaggregate the combined effects of sex with age and body mass index (bmi) in the n ab response.Differences between the pre- or post-vaccination geometric mean n ab titers between male and female hcws were not observed.Male hcws were 2.86 times more likely to seroconvert compared to female hcws in 2017-2018, but the same trend was not observed in the following year.When data were disaggregated by age and sex, older female hcws had higher h1n1 pre- and post-vaccination n ab titers compared to male hcws in the same age group for both vaccination campaign seasons.In both years, the decline in h3n2 pre-vaccination titers with increasing bmi was greater in female than male hcw.The sex-specific effects of age and bmi on n ab responses to seasonal influenza vaccines require greater consideration.

Journal: Vaccine (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural
Artificial intelligence for prediction of treatment outcomes in breast cancer: Systematic review of design, reporting standards, and bias.

Artificial intelligence (ai) has the potential to personalize treatment strategies for patients with cancer.However, current methodological weaknesses could limit clinical impact.We identified common limitations and suggested potential solutions to facilitate translation of ai to breast cancer management.Methods a systematic review was conducted in medline, embase, scopus, google scholar and pub med central in july 2021.Studies investigating the performance of ai to predict outcomes among patients undergoing treatment for breast cancer were included.Algorithm design and adherence to reporting standards were assessed following the transparent reporting of a multivariable prediction model for individual prognosis or diagnosis (tripod) statement.Risk of bias was assessed by using the prediction model risk of bias assessment tool (probast), and correspondence with authors to assess data and code availability.Results our search identified 1,124 studies, of which 64 were included: 58 had a retrospective study design, with 6 studies with a prospective design.Access to datasets and code was severely limited (unavailable in 77% and 88% of studies, respectively).On request, data and code were made available in 28% and 18% of cases, respectively.Ethnicity was often under-reported (not reported in 52 of 64, 81%), as was model calibration (63/64, 99%).The risk of bias was high in 72% (46/64) of the studies, especially because of analysis bias.Conclusion development of ai algorithms should involve external and prospective validation, with improved code and data availability to enhance reliability and translation of this promising approach.Protocol registration number: prospero - crd42022292495.

Journal: Cancer treatment reviews (2022)
Publication types: Journal Article;Review;Systematic Review
Daytime eating prevents mood vulnerability in night work.

Shift workers have a 25 to 40% higher risk of depression and anxiety partly due to a misalignment between the central circadian clock and daily environmental/behavioral cycles that may negatively affect mood and emotional well-being.Hence, evidence-based circadian interventions are required to prevent mood vulnerability in shift work settings.We used a stringently controlled 14-d circadian paradigm to assess mood vulnerability during simulated night work with either daytime and nighttime or daytime-only eating as compared with simulated day work (baseline).Simulated night work with daytime and nighttime eating increased depression-like mood levels by 26.2% (p-value adjusted using false discovery rates,p fdr = 0.001; effect-sizer= 0.78) and anxiety-like mood levels by 16.1% (p fdr = 0.001; effect-sizer= 0.47) compared to baseline, whereas this did not occur with simulated night work in the daytime-only eating group.Importantly, a larger degree of internal circadian misalignment was robustly associated with more depression-like (r= 0.77;p= 0.001) and anxiety-like (r= 0.67;p= 0.002) mood levels during simulated night work.These findings offer a proof-of-concept demonstration of an evidence-based meal timing intervention that may prevent mood vulnerability in shift work settings.Future studies are required to establish if changes in meal timing can prevent mood vulnerability in night workers.

Journal: Proceedings of the National Academy of Sciences of the United States of America (2022)
Publication types: Journal Article;Randomized Controlled Trial;Research Support, N.I.H., Extramural;Research Support, Non-U.S. Gov't
The Modified Sternoplasty: A Novel Surgical Technique for Treating Mediastinitis.

Deep sternal wound infection (dswi) is one of the most complex and devastating complications post cardiac surgery.We present here the modified sternoplasty, a novel surgical technique for treating dswi post cardiac surgery.The modified sternoplasty includes debridement and sternal refixation via bilateral longitudinal stainless-steel wires that are placed parasternally along the ribs at the midclavicular or anterior axillary line, followed by six to eight horizontal stainless-steel wires that are anchored laterally and directly into the ribs.On top of that solid structure, wound reconstruction is performed by the use of bilateral pectoralis muscle flaps followed by subcutaneous tissue and skin closure.We reported mortality rates and length of hospitalization of patients who underwent the modified sternoplasty.In total, 68 patients underwent the modified sternoplasty.Two of these critically ill patients died (2.9%).The average length of hospitalization from the diagnosis of dswi was 24.63 ± 22.09 days.The modified sternoplasty for treating dswi is a more complex surgery compared with other conventional sternoplasty techniques.However, this technique was demonstrated to be more effective, having a lower rate of mortality, and having a length of hospitalization lower than or comparable to other techniques previously reported in the literature.

Journal: Plastic and reconstructive surgery. Global open (2022)
Publication types: Journal Article