syedzayyan/paper_mill_benchmark huggingface dataset:
Analysis of Related Factors Affecting Facial Nerve Function after Acoustic Neuroma Surgery

To investigate the factors affecting facial nerve function after acoustic neuroma surgery and to provide theoretical reference for clinicians to preserve facial nerve function better after surgery.Methods.A retrospective cohort study was conducted to analyze the correlation between postoperative facial nerve function and surgical approach, age, sex, tumor size and adhesion degree of facial nerve in 152 patients with acoustic neuroma.Results.In the choice of surgical approach, there was no significant difference in the anatomy of the complete facial nerve in labyrinth path, retrosigmoid sinus path, and middle cranial fossa path.There was no statistically significant difference between the middle cranial fossa path and the retrosigmoid sinus path in facial nerve function preservation 7 days after surgery.The difference between middle cranial fossa path and labyrinthine path was statistically significant ( p &lt; 0.01 ).There were statistically significant differences between labyrinth path and retrosigmoid sinus path ( p &lt; 0.05 ).Logistic multivariate regression analysis showed that the operative approach and the degree of adhesion between tumor and facial nerve were the risk factors affecting functional preservation of facial nerve 7 days after surgery.Age and the degree of adhesion between tumor and facial nerve were the risk factors for functional preservation of facial nerve 1 year after operation.Conclusion.The facial nerve function injury in patients with acoustic neuroma may be related to the choice of surgical approach, the adhesion degree of tumor and facial nerve, and their age.Clinicians need to comprehensively evaluate the risk factors before surgery, so as to achieve individualized treatment to protect the integrity of postoperative facial nerve function of patients.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;
KL-FGF23-VD Axis in Improving Late-Onset Alzheimer’s Disease by Modulating IKK/NF-κB Signal Pathway

Of the study.Late-onset alzheimer’s disease (load) is recognized as a degeneration disorder in patients’ central nervous system over 65 years old who will experience memory decline and cognitive impairment, causing great harm to the health of the elderly.To investigate the role of kl-fgf23-vd axis in load and its molecular mechanism, we designed experiments from two dimensions of cells and animals.Materials and methods.Load rats and aβ microglia were constructed by using aβ1-40 and ibo mixture.The effect of kl-fgf23-vd axis on load was investigated by transfecting overexpressing and interfering with kl gene adenovirus, and ikk-16 was added to aβ microglia to explore the effect of kl-fgf23-vd axis on regulation of ikk/nf-κb signaling pathway.Results.The results showed that, in kl-oe group, fgf23 was decreased in the hippocampus of load rats compared with control and kl-si, and the trend was opposite in the kl-si group.The kl-fgf23-vd axis can alleviate inflammatory response, reduce the deposition of aβ, and inhibit activation of the nf-κb pathway and neuron apoptosis in brain tissue of load rats.In aβ microglia, the expression of kl-fgf23-vd axis was consistent with animal experiments.The kl-fgf23-vd axis can inhibit the expression of aβ microglia inflammatory factors and the activation of microglia and nf-κb pathway.Meanwhile, ikk expression was decreased in kl-oe group compared with kl-si and control.In the ikk-16 addition group, the ability of kl-fgf23-vd axis to inhibit the activation of microglia and nf-κb pathway was enhanced.Conclusions.These findings suggest a potential role of the kl-fgf23-vd axis in ad treatment by regulating the ikk/nf-κb pathway.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;
CircKMT2E Participates in Osteoarthritis through Promotes Apoptosis of Chondrocytes Via Sponging miR-140-5p to Activate TLR4

To explore the latent pathogenesis of circ rnas in osteoarthritis (oa), as well as their function mechanism.Methods.The murine chondrocytes with and without oa were involved and used for in-depth sequencing.Herein, we carried out subsequent bioinformatics analysis to disclose the expression pattern, characteristics of circ rnas based on gene ontology, and the kegg pathway analyses.Then sequencing data were used to deduce the interaction between circ rna and mi rna.The potential mi rna response elements for the annotated circ rnas and relevant target genes were forecasted on the basis of target scan and mi randa.For chondrocytes, the effect of the overexpression of the screened circ rna for apoptosis was spotted by flow cytometry as well as western blot.Results.466 diverse circ rnas in the 23,787 spotted circ rnas were both significantly and differentially transcribed.Circ kmt2e was upregulated more than two folds in chondrocytes with oa compared with normal tissues, exhibiting an expression trend opposite to mi r-140-5p.We disclosed that circ kmt2e could possess mutual effect with mi r-140-5p by way of ago proteins.Thus, circ kmt2e was verified to have functioned as a molecular sponge targeting mi r-140-5p.Therefore, circ kmt2e may be at work in the pathogenesis of oa.Further, the sponge connection between circ kmt2e and mi r-140-5p was proved on the basis of a dual-luciferase reporter assay.Besides, mi r-140-5p was speculated can bind tlr4 by bioinformatics analysis.Further pcr analysis found the relative expression level of tlr4, caspase-3, and bax in the oa groups presented significant upregulation.Overexpression of circ kmt2e can promote apoptosis of chondrocytes.Conclusion.The upregulation of circ kmt2e is involved in the chondrocyte apoptosis of the pathogenesis of oa through activation of tlr4 by the sponge function of mi r-140-5p.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;
Comparison of the Efficacy of Different Insulin Administration and Blood Glucose Monitoring Methods in the Treatment of Type 1 Diabetes Mellitus in Children

To compare the clinical efficacy of different insulin administration methods and blood glucose monitoring methods in treating type 1 diabetes mellitus in children.Methods.Patients were divided into four groups: multiple daily injection (mdi) + fingertip blood glucose detection, continuous subcutaneous insulin infusion (csii) + fingertip blood glucose detection, mdi + continuous glucose monitoring system (cgms), and csii + cgms.After six months of treatment, followed by telephone and at least once a month in an outpatient clinic, insulin doses were adjusted according to the children’s blood glucose levels.Blood glucose control and the daily dose of insulin were compared among the four groups after treatment, and the incidence of hypoglycemia in each group was recorded during the treatment.We also compare the incidence of the adverse event among the four groups.Results.6 months later, the levels of hb a1c, fbg, and two h pg in each group were lower than those before treatment.There were significant differences in hb a1c, two h pg, and the daily insulin dose among the four groups.There were differences in the frequency of hypoglycemia among all the groups.The frequency of hypoglycemia in groups c and d was lower than in group a.Conclusions.Csii was better than mdi, and the blood glucose monitoring effect of cgms was better than the fingertip blood glucose detection.The patients treated with csii combined with cgms had the best clinical efficacy.The patients treated with csii combined with cgms had the lowest adverse events incidence.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Clinical Study;
Effect of HCH Nutrition Management Combined with Early Exercise Nursing on Nutrition Status and Postoperative Rehabilitation of Patients after Gastric Cancer Surgery

The study aimed to analyze the effect of hospital-community-family (hch) nutrition management combined with early exercise nursing on nutrition status and postoperative rehabilitation of patients after gastric cancer surgery.Methods.A total of 80 patients with gastric cancer admitted from january 2019 to june 2021 were selected and divided by the odd-even grouping method into two groups, i.e., 40 cases in the control group adopting routine nursing combined with early exercise nursing and 40 cases in the study group adopting hch nutrition management; the nutrition status and postoperative rehabilitation of the two groups were compared.Results.The times of feeding, bowel sound, anal exhaust, and defecation in the study group after surgery were shorter than those in the control group, with a difference of statistical significance ( p &lt; 0.05 ).3 months after discharge, the scores of patient-generated subjective global assessment (pg-sag) in the study group were lower than those in the control group, while the body mass index (bmi), prealbumin, albumin, hemoglobin, and the score of the gastrointestinal quality of life index (giqli) were higher than those of the control group ( p &lt; 0.05 ).Conclusion.Hospital-community-family nutrition management combined with early exercise nursing can improve the postoperative nutrition status of patients undergoing gastric cancer surgery and advance the time of postoperative exhaust and defecation.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;
LncRNA Gm43843 Promotes Cardiac Hypertrophy via miR-153-3p/Cacna1c Axis

Long noncoding rnas (lnc rnas) have been reported to engage in many human diseases, including cardiac hypertrophy.Cardiac hypertrophy was mainly caused by excessive pressure load, which can eventually lead to a decline in myocardial contractility.Gm43843, a novel lnc rna, has not been well explored in cardiac hypertrophy so far.Herein, we are going to search the function and the underlying molecular mechanism of gm43843 in cardiac hypertrophy.Gm43843 levels were measured via q rt-pcr in mouse myocardial cells when they are treated with angiogenin ii (ang ii) or transfected with different plasmids.Western blot assay was implemented to detect the cardiac hypertrophy-related protein markers, while the cell was analyzed via immunofluorescence (if) assay to evaluate the hypertrophy.Meanwhile, the binding of gm43843 and the putative targets was examined based on mechanistic assay results.We found that gm43843 expression was increased with the elevated concentration of ang ii.Inhibited gm43843 was detected to reduce the hypertrophy of mouse myocardial cells.Meanwhile, gm43843/mi r-153-3p/cacna1c axis was found to modulate cardiac hypertrophy.In short, gm43843 promotes cardiac hypertrophy via mi r-153-3p/cacna1c axis.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;
Noncoding RNAs and Virus and Treatment in Allergic Rhinitis

Allergic rhinitis (ar) is a type i hypersensitivity reaction disease caused by inhaled allergens and immunoglobulin e (ig e)-mediated.Noncoding rna (nc rna) is an important regulator involved in gene expression and can be detected in the cytoplasm or extracellular fluid, which mainly includes micro rnas (mi rna, length 22–24 nucleotides), long noncoding rnas (lnc rna, length &gt;200 nucleotides), and circ rnas.Lnc rna and mi rna both participate in the regulation of immune function.Some respiratory viral infections can aggravate allergic rhinitis, such as a respiratory syncytial virus (rsv) and human metapneumovirus (h mpv).However, the interaction between viral infection and allergy is complex and the mechanism is still unclear.In this review, we summarized the interactions of noncoding rnas and viruses in the occurrence and development of ar, along with the treatments focusing on the noncoding rnas in the past five years.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Review Article;
HDAC2 Induces DNA Methyltransferase DNMT3B Expression to Regulate the Wnt Signaling Pathway and Thus Promotes Glioma Development and Progression

To investigate the role and molecular mechanism of hdac2 in glioma.Methods.Gse16011, gse31262, and gse90598 datasets were used to identify co-expressed genes, go analysis, and kegg analysis to identify gene enrichment pathways, and ppi networks were constructed to identify gene interrelationships.Hdac2 enrichment on dnmt3b promoter and dnmt3b enrichment on bcl2 cp g island was detected by a ch ip assay.The expression, prognosis, and hierarchical distribution of hdac2, dnmt3b, and bcl2 were examined in the cgga database, and the correlation between hdac2 and dnmt3b, bcl2, and dnmt3b and bcl2 was assessed.Results.The hdac2-dnmt3b-bcl2 axis is differentially expressed and interacts in gliomas.Hdac2 activates the transcriptional activity of dnmt3b, and dnmt3b inhibits the expression of bcl2.Hdac2 and dnmt3b are highly expressed in gliomas and have a poor prognosis, while bcl2 is lowly expressed in gliomas and has a good prognosis.Conclusion.Hdac2 promotes dnmt3b transcriptional repression of bcl2 expression and wnt pathway activity, thereby activating glioma cell activity in vitro and in vivo.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;
A Three-Year Prospective Study Assessing the Application of Chromosomal Microarray Analysis in 576 High-Risk Pregnant Women

The use of chromosomal microarray analysis (cma) in prenatal diagnosis of chromosomal and genetic diseases has resulted in a significant improvement in the diagnosis of genetically caused congenital malformations, neurodevelopmental disorders, and congenital anomalies, with a high diagnostic yield in selected prenatal cases.Objective.The objective of this study was to evaluate the application of cma in the prenatal diagnosis of high-risk pregnant women.Method.A total of 576 pregnancies were selected from may 2018 to october 2020 in our hospital, including amniotic fluid chromosome, karyotype analysis, and cma detection.The study group was divided into two groups based on the indications for testing: group a has 88 patients at the age of 35 years or older, and group b patients were in high-risk pregnancies, which consisted of 33 cases of bad pregnancy history, 252 high-risk serological screenings, 70 high-risk non-invasive prenatal testing (nipt), 65 cases of b-ultrasound indicated fetal development abnormalities or ultrasonic soft marker abnormalities, and 68 other cases of pregnant women or both who have genetic or chromosomal abnormalities.At last, we have an analysis of the detection rate from different testing methods.Results.Based on the follow-up test, 576 high-risk pregnant women showed an amniotic fluid chromosome karyotype rate of 18.1% (104/576), and the remaining 472 of these cases suffered a cnv ratio of 14.2% (67/472).472 women of low clinical relevance are at 4.87% (23/472), 16 people showed a clear cause ratio = 3.39% (16/472), and 28 of the 472 (5.93%) cases showed polymorphism.Conclusions.In our study, cma significantly improved the fetal detection rate and diagnosis rate in high-risk pregnant women, which proved to be a very useful method in the diagnosis of genetically caused neurodevelopmental disorders and congenital anomalies.The use of cma in high-risk pregnant women is justified, and these women can detect an additional (3.40%, 16/472) of pathogenic microdeletions and microduplications in the cases.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;
To Systematically Evaluate and Analyze the Efficacy and Safety of Transcatheter Arterial Chemoembolization (TACE) in the Treatment of Primary Liver Cancer

The efficacy and safety of transcatheter arterial chemoembolization (tace) are systematically evaluated in the treatment of primary liver cancer, which provides a reference for clinical practice and more in-depth research.Cochrane library, pub med, embase, cbm, cnki, vip, and wan fang data, supplemented by other searches, collected all randomized controlled trials (rct) comparing tace combined with tace alone for hcc.The meta-analysis, after selecting the literature, extracting data, and evaluating the methodological quality of the included studies following the inclusion criteria, was performed using rev man 5.1 software.There was statistical difference in 3-year survival rate of tace combined with heat treatment for advanced hepatocellular carcinoma (or = 1.72,95%ci (1.22,2.41), p = 0.002 , i2 = 0%, and z = 3.12), total effective rate (or = 1.91,95%ci (1.31,2.78), p = 0.0008 , i2 = 0%, and z = 3.37), quality-of-life improvement rate (or = 2.29,95%ci (1.62,3.23), p &lt; 0.00001 , i2 = 83%, and z = 3.37), and complication rate (or = 2.29,95%ci (1.62,3.23), p &lt; 0.00001 , i2 = 83%, and z = 3.37).Compared with tace alone, tace combined with hyperthermia can significantly improve the survival rate and recent efficacy of patients, improve the quality of life, and have a trend to reduce the incidence of toxicity.However, its long-term efficacy and more comprehensive safety need to be verified by more sample and high-quality rct.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
The Trend of Burn Injury Patients in Ningbo between 2012 and 2021: A Clinical Study

This study was designed to understand the local changes of burn injuries in recent 10 years, so as to provide reliable reference data and viewpoints for prevention and vigilance of local burn injuries.Methods.In this study, 184 patients with a burn injury admitted to our hospital from 2012 to 2021 were enrolled and analyzed retrospectively.According to their information in the electronic database, the number of patients with burn injuries and the location of each disaster each year were analyzed, and the age, sex, hospital stay and hospitalization expense of each patient were collected.With 5 years as the boundary, the patients were divided into a 2012–2016 group and a 2017–2021 group and the differences of the two groups in the abovementioned aspects were compared.Results.During 2012–2021, the incidence rate of burn injuries in men was higher than that in women and workplaces had a higher burn injury rate than residents’ homes.Compared with the period of 2012–2016, the number of fires or explosions and the number of patients with a burn injury during 2017–2028 both increased, but there was no significant change in disaster location, male-female ratio, age, average hospital stay, and average hospitalization expense.Conclusion.In the face of the increasing prevalence of burn injuries, we should strengthen fire-fighting knowledge-related education and fire prevention management and actively explore post-burn injury treatment strategies and potential treatment targets to promote the development of burn injury management and treatment strategies.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;
Application of Artificial Intelligence Nuclear Medicine Automated Images Based on Deep Learning in Tumor Diagnosis

In order to correctly obtain normal tissues and organs and tumor lesions, the research on multimodal medical image segmentation based on deep learning fully automatic segmentation algorithm is more meaningful.This article aims to study the application of deep learning-based artificial intelligence nuclear medicine automated images in tumor diagnosis.This paper studies the methods to improve the accuracy of the segmentation algorithm from the perspective of boundary recognition and shape changeable adaptive capabilities, studies the active contour model based on boundary constraints, and proposes a superpixel boundary-aware convolution network to realize the automatic ct cutting algorithm.In this way, the tumor image can be cut more accurately.The experimental results in this paper show that the improved algorithm in this paper is more robust than the traditional ct algorithm in terms of accuracy and sensitivity, an increase of about 12%, and a slight increase in the negative prediction rate of 3%.In the comparison of cutting images of malignant tumors, the cutting effect of the algorithm in this paper is about 34% higher than that of the traditional algorithm.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Sufentanil Alleviates Sepsis-Induced Myocardial Injury and Stress Response in Rats through the ERK/GSK-3β Signaling Axis

To explore the effect and possible mechanism of sufentanil on sepsis-induced myocardial injury and stress response in rats.Methods.The cecal ligation and puncture (clp) method was utilized to establish the sepsis model of rats to explore the effect of sufentanil pretreatment with different concentrations on myocardial injury and oxidative stress in clp rats.Echocardiogram was applied for detecting cardiac hemodynamic parameters in rats; hematoxylin and eosin (he) staining as well as tunel staining was done for observing pathological changes of myocardial tissue and cardiomyocyte apoptosis in rats, respectively; biochemical testing and enzyme-linked immunosorbent assay (elisa) were done for determining myocardial injury marker level in serum, oxidative stress substances in myocardial tissue, and neuroendocrine hormone level in serum of rats, respectively; finally, western blot was performed for checking the expression level of erk/gsk-3β signaling pathway-related proteins in myocardial tissue of rats.Results.A model of rat with sepsis-induced myocardial injury was constructed with the clp method.Specifically, this rat model was characterized by obvious cardiac function and tissue damage, cardiomyocyte apoptosis, and oxidative stress response.Sufentanil pretreatment significantly improved cardiac function injury, alleviated pathological injury and oxidative stress response in myocardial tissue, and inhibited cardiomyocyte apoptosis.Specifically, after sufentanil pretreatment, left ventricular end-diastolic dimension (lvedd) and left ventricular end-systolic dimension (lvesd) were downregulated, and left ventricular ejection fraction (lvef) was upregulated; the level of b-type natriuretic peptide (bnp) of serum, creatine kinase isoenzyme (ck-mb), and troponin (c tnl) decreased; besides, malondialdehyde (mda) level was declined, while activities of superoxide dismutase (sod) and catalase (cat) were increased.What is more, further mechanism exploration also revealed that sufentanil could reverse the activity of the sepsis-induced erk/gsk-3β signaling pathway.Conclusion.Sufentanil has an obvious protective effect on myocardial injury and stress response in clp rats, and this protective effect may be related to the activation of the erk/gsk-3β signaling pathway.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;