syedzayyan/paper_mill_benchmark huggingface dataset:
To Analyze the Mechanism of SalB Regulating SIRT1 to Inhibit NLRP3 and Its Ameliorative Effect on Tubulogastric Junction Tumor Lesions Complicated with Myocardial Injury

The objective of this research is to investigate the mediating impact of salvianolic acid b (sal b) on sirt1 signaling pathway and the mechanism by which it inhibits nod‐like receptor protein 3 (nlrp3), as well as to examine how sal b affects myocardial injury brought on by tumor lesions at the junction of the tube and the stomach.Through the establishment of the integration of a stomach tube tumor lesion rats combined with the experimental rat model, this study establishes the normal group, model group, and different sal b dose groups.For each group of cells, cell activity and cell apoptosis were determined and compared using colorimetry and enzyme‐linked immunosorbent method about lactate dehydrogenase (ldh).Interleukin‐1 beta levels are measured.Dcfh‐da fluorescent probe was applied to identify intracellular “reactive oxygen species” (ros).“western blot” was used to determine nlrp3, caspase‐1, and apoptosis‐related spotted protein (asc) in each group of cells.And sirt1 signaling pathway related to sirt1, phosphorylated amp protein‐activated kinase α (p‐ampk α ), amp protein‐activated kinase α (ampk α ), and “peroxisome‐proliferator‐activated receptor γ coactivator 1 α (pgc‐1 α ) protein expression” are used.According to the final findings, sal b mediated the sirt1 signaling pathway and had a beneficial impact on the upregulation of sirt1, p‐ampk/ampk, and pgc‐1 protein expressions.Sal b positively affects the downregulation of nlrp3 inflammasome‐related proteins.Caspase‐1 and asc protein expression suggesting that sal b may inhibit the activation of nlrp3 inflammasome induced by oxidative stress by activating sirt1/ampk/pgc‐1 α signaling pathway.This plays an antimyocardial injury effect.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Thyme Oil and Thymol Counter Doxorubicin-Induced Hepatotoxicity via Modulation of Inflammation, Apoptosis, and Oxidative Stress

Doxorubicin (dox) is an effective anticancer agent with a wide spectrum of activities.However, it has many adverse effects on various organs especially on the liver.Thymol, one of the major components of thyme oil, has biological properties that include anti-inflammatory and antioxidant activities.Thus, this study was designed to examine thyme oil and thymol for their ability to prevent doxorubicin-induced hepatotoxicity in wistar rats.Hepatotoxicity was induced by an intraperitoneal injection of doxorubicin, at a dose of 2 mg/kg bw/week, for seven weeks.Doxorubicin-injected rats were supplemented with thyme oil and thymol at doses 250 and 100 mg/kg bw, respectively, four times/week by oral gavage for the same period.Treatment of rats with thyme oil and thymol reversed the high serum activities of ast, alt, and alp and total bilirubin, afp, and ca19.9 levels, caused by doxorubicin.Thyme oil and thymol also reduced the high levels of tnf-α and the decreased levels of both albumin and il-4.These agents ameliorated doxorubicin-induced elevation in hepatic lipid peroxidation and associated reduction in gsh content and gst and gpx activities.Further, the supplementation with thyme oil and thymol significantly augmented m rna expression of the level of antiapoptotic protein bcl-2 and significantly downregulated nuclear and cytoplasmic levels of the hepatic apoptotic mediator p53.Thus, thyme oil and thymol successfully counteracted doxorubicin-induced experimental hepatotoxicity via their anti-inflammatory, antioxidant, and antiapoptotic properties.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Theaflavin Chemistry and Its Health Benefits

Huge epidemiological and clinical studies have confirmed that black tea is a rich source of health‐promoting ingredients, such as catechins and theaflavins (tfs).Furthermore, tf derivatives mainly include theaflavin (tf1), theaflavin‐3‐gallate (tf2a), theaflavin‐3 ′ ‐gallate (tf2b), and theaflavin‐3,3 ′ ‐digallate (tf3).All of these tfs exhibit extensive usages in pharmaceutics, foods, and traditional medication systems.Various indepth studies reported that how tfs modulates health effects in cellular and molecular mechanisms.The available literature regarding the pharmacological activities of tfs has revealed that tf3 has remarkable anti‐inflammatory, antioxidant, anticancer, antiobesity, antiosteoporotic, and antimicrobial properties, thus posing significant effects on human health.The current manuscript summarizes both the chemistry and various pharmacological effects of tfs on human health, lifestyle or aging associated diseases, and populations of gut microbiota.Furthermore, the biological potential of tfs has also been focused to provide a deeper understanding of its mechanism of action.

Journal: Oxidative Medicine and Cellular Longevity (2021)
Publication types: Review Article;
The Visceral Adiposity Index (VAI) and Lipid Accumulation Product (LAP) Are Predictors of Insulin Resistance and Hyperandrogenaemia in Obesity/Overweight Women with Polycystic Ovary Syndrome

Hyperandrogenaemia and insulin resistance (ir) are the main characteristics of polycystic ovary syndrome (pcos).Here, we study to find appropriate markers predicting ir and hyperandrogenaemia of women with pcos in northwest china.Methods .According to body mass index (bmi), 953 patients with pcos were divided into two groups.All the patients underwent physical examination and ultrasonography and collected elbow vein blood.Their bmi, waist‐to‐height ratio (wht r), waist‐to‐hip ratio (whr), lap, vai, homeostasis model assessment index of insulin resistance (homa‐ir), and free androgen index (fai) were calculated.Each group (normal weight and obesity/overweight) was further divided into two subgroups according to their homa‐ir and fai: the ir+ subgroup/ir‐ subgroup and fai+ subgroup/fai‐ subgroup.Furthermore, we compared the clinical indices, hormone levels, and metabolic makers separately between these groups.The correlations between these parameters and homa‐ir or fai were tested; sensitivity, specificity, and receiver‐operating characteristic (roc) curves were calculated.Results .In the obesity/overweight group, the vai (best cut‐off value: 2.27, area under the curve (auc) = 0.699) and lap (best cut‐off value: 45.54, auc = 0.680) were sensitive predictors of ir (sensitivity = 72 % and sensitivity = 67 % ).Additionally, the vai (best cut‐off value: 2.13, auc = 0.624) and lap (best cut‐off value: 51.18, auc = 0.582) were sensitive predictors of fai (sensitivity = 87 % and sensitivity = 64 % ).In the normal weight group, bmi could preferably predict homa‐ir (auc = 0.717, best cut‐off value: 21.62) and homa‐ir could preferably predict fai (best cut‐off value: 2.11, auc = 0.648).Conclusion .Our data indicated that the vai and lap may contribute to the early identification of ir and hyperandrogenaemia in the obesity/overweight patients of pcos.In normal weight pcos, bmi was a better predictor to ir, and howa‐ir was a better predictor to fai.

Journal: BioMed Research International (2023)
Publication types: Research Article;
The Use of BT-ESD Technology under General Intravenous Anesthesia in the Treatment of Nonmuscle Invasive Bladder Cancer and the Effect of PI3K/Akt Signaling Pathway on Tumor Recurrence

The objective is to investigate the effect of minimally invasive treatment of nonmuscle invasive bladder cancer (nmibc) by transurethral endoscopic submucosal dissection of bladder tumor (bt‐esd) under general intravenous anesthesia and the expression of akt in nmibc and to study the effect of upregulation of intracellular phosphatidylinositol kinase (pi3k)/protein kinase b (akt) on tumor recurrence.Method .130 patients with nmibc were selected as the research subjects, including 101 males and 29 females.The patients were divided into transurethral resection of bladder tumor (turbt) group, bt‐esd learning group a (early 20 cases of chief surgeon), and bt‐esd learning group b (follow‐up cases of the chief surgeon).The general information (male and female prevalence ratio and average age), operation duration, postoperative bladder irrigation duration, postoperative indwelling catheter time, postoperative hospitalization time, and postoperative complications were compared among the patients in all groups.The normal bladder tissues and pathological tissues of nmibc patients were stained by immunohistochemistry.Results .No significant difference is identified in age among the three groups ( p &gt; 0.05), but there are significant differences in the operation duration, postoperative bladder irrigation time, postoperative indwelling catheter time, and postoperative hospital stay ( p &gt; 0.05).At the same time, compared with bt‐esd group a and bt‐esd group b, the number of postoperative complications in turbt group is statistically different ( p &lt; 0.05).Akt has a strong positive expression in the nucleus of patients, which indicates that akt activated by cellular pi3k will form the pi3k/akt signaling pathway and reduce the recurrence rate of bladder tumor.

Journal: BioMed Research International (2021)
Publication types: Clinical Study;
An Improved COVID-19 Detection using GAN-Based Data Augmentation and Novel QuNet-Based Classification

Covid‐19 is a fatal disease caused by the sars‐co v‐2 virus that has caused around 5.3 million deaths globally as of december 2021.The detection of this disease is a time taking process that have worsen the situation around the globe, and the disease has been identified as a world pandemic by the who.Deep learning‐based approaches are being widely used to diagnose the covid‐19 cases, but the limitation of immensity in the publicly available dataset causes the problem of model over‐fitting.Modern artificial intelligence‐based techniques can be used to increase the dataset to avoid from the over‐fitting problem.This research work presents the use of various deep learning models along with the state‐of‐the‐art augmentation methods, namely, classical and generative adversarial network‐ (gan‐) based data augmentation.Furthermore, four existing deep convolutional networks, namely, dense net‐121, inception v3, xception, and res net101 have been used for the detection of the virus in x‐ray images after training on augmented dataset.Additionally, we have also proposed a novel convolutional neural network (qu net) to improve the covid‐19 detection.The comparative analysis of achieved results reflects that both qu net and xception achieved high accuracy with classical augmented dataset, whereas qu net has also outperformed and delivered 90% detection accuracy with gan‐based augmented dataset.

Journal: BioMed Research International (2022)
Publication types: Research Article;
The Therapeutic Roles of Cinnamaldehyde against Cardiovascular Diseases

Evidence from epidemiological studies has demonstrated that the incidence and mortality of cardiovascular diseases (cvds) increase year by year, which pose a great threat on social economy and human health worldwide.Due to limited therapeutic benefits and associated adverse effects of current medications, there is an urgent need to uncover novel agents with favorable safety and efficacy.Cinnamaldehyde (ca) is a bioactive phytochemical isolated from the stem bark of chinese herbal medicine cinnamon and has been suggested to possess curative roles against the development of cvds.This integrated review intends to summarize the physicochemical and pharmacokinetic features of ca and discuss the recent advances in underlying mechanisms and potential targets responsible for anti-cvd properties of ca.The ca-related cardiovascular protective mechanisms could be attributed to the inhibition of inflammation and oxidative stress, improvement of lipid and glucose metabolism, regulation of cell proliferation and apoptosis, suppression of cardiac fibrosis, and platelet aggregation and promotion of vasodilation and angiogenesis.Furthermore, ca is likely to inhibit cvd progression via affecting other possible processes including autophagy and er stress regulation, gut microbiota and immune homeostasis, ion metabolism, nc rna expression, and trpa1 activation.Collectively, experiments reported previously highlight the therapeutic effects of ca and clinical trials are advocated to offer scientific basis for the compound future applied in clinical practice for cvd prophylaxis and treatment.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Review Article;
Unani Formulation Habb-e-Suranjan: A Treasure of Biological Activities

Habb‐e‐suranjan (hes), an unani formulation, has been studied for its anti‐inflammatory properties in both in vitro and in vivo experiments.Hes is recommended for arthritis, gout, and joint pain.The current endeavor is an attempt to put it to the test and verify its efficacy scientifically.It was tested for dpph, hydroxyl, and nitric oxide scavenging activities.It was shown that hes had the greatest tac and frac values when compared to catechin and ascorbic acid.Hes exhibited dpph and hydroxyl radical scavenging activity that was dose‐dependent.Incubation of sodium nitroprusside solutions in pbs at 25°c for 150 min resulted in the production of nitric oxide.Nitric oxide production was effectively decreased by hes.Anti‐inflammatory medications boosted the migration of pmn cells toward the chemoattractant fmlp in an agarose experiment of pmn chemotaxis.In carrageenan‐induced rat paw edema, in the hes‐treated group, paw thickness was 3.021 ± 0.084 at t = 0, but it showed an increase in paw inflammation after one hour, i.e., 3.195 ± 0.082 cm which again showed a decrease in paw thickness up to 4th hour, i.e., 3.018 ± 0.078, 2.98 ± 0.032, and 2.684 ± 0.061 at t = 2, 3, and 4, respectively.It showed again getting back to the normal thickness of paw at t = 24 hrs, i.e., 3.029 ± 0.118 cm.It is concluded that the formulation is potent enough and can be used effectively for the treatment of inflammation and associated health issues.Moreover, there is much scope to evaluate its effectiveness using different in vitro and in vivo models.

Journal: BioMed Research International (2022)
Publication types: Research Article;
The Study on the Pathogenesis of Pediatric Lymphoma Based on the Combination of Pseudotargeted and Targeted Metabolomics

Pediatric lymphoma is a kind of malignant tumor with high mortality.The complexity of pediatric lymphoma shows a great challenge for effective diagnosis and treatment.In order to meet the challenge, the combination of pseudotargeted and targeted metabolomics was used to analyze the serum metabolites in pediatric lymphoma patients and healthy controls for discovering the metabolites related to pediatric lymphoma.The serum samples were obtained from the treatment group ( n = 43), the control group ( n = 26), and the patients group ( n = 18).A total of 17 serum metabolites, including carnitine, leucine, creatine, urea, (6z,9z,12z)‐octadecatrienoic acid, linoleate, octadecenoic acid, l‐palmitoylcarnitine, hexadecanoic acid, tetradecanoic acid, (9z)‐hexadecenoic acid, uric acid, glucose, 1‐methylnicotinamide, hypoxanthine, l‐glutamine, and taurine, were found to be related to pediatric lymphoma.They could provide a scientific diagnostic basis and therapeutic target for pediatric lymphoma and elucidate the mechanism of pediatric lymphoma.

Journal: BioMed Research International (2021)
Publication types: Clinical Study;
The Risk Model Based on the Three Oxidative Stress-Related Genes Evaluates the Prognosis of LAC Patients

Oxidative stress plays a role in carcinogenesis.This study explores the roles of oxidative stress-related genes (osrgs) in lung adenocarcinoma (lac).Besides, we construct a risk score model of osrgs that evaluates the prognosis of lac patients.Methods.Osrgs were downloaded from the gene set enrichment analysis (gsea) website.The expression levels of osrgs were confirmed in lac tissues of the tcga database.Go and kegg analyses were used to evaluate the roles and mechanisms of oxidative stress-related differentially expressed genes (degs).Survival, roc, cox analysis, and aic method were used to screen the prognostic degs in lac patients.Subsequently, we constructed a risk score model of osrgs and a nomogram.Further, this work investigated the values of the risk score model in lac progression and the relationship between the risk score model and immune infiltration.Results.We discovered 163 oxidative stress-related degs in lac, involving cellular response to oxidative stress and reactive oxygen species.Besides, the areas under the curve of ccna2, cdc25c, ero1a, cdk1, plk1, itgb4, and gjb2 were 0.970, 0.984, 0.984, 0.945, 0.984, 0.771, and 0.959, respectively.This indicates that these osrgs have diagnosis values of lac and are significantly related to the overall survival of lac patients.Ero1a, cdc25c, and itgb4 overexpressions were independent risk factors for the poor prognosis of lac patients and were associated with risk scores in the risk model.High-risk score levels affected the poor prognosis of lac patients.Notably, a high-risk score may be implicated in lac progression via cell cycle, dna replication, mismatch repair, and other mechanisms.Further, ero1a, cdc25c, and itgb4 expression levels were related to the immune infiltrating cells of lac, including mast cells, nk cells, and cd8 t cells.Conclusion.In summary, ero1a, cdc25c, and itgb4 of osrgs are associated with poor prognosis of lac patients.We confirmed that the risk model based on the ero1a, cdc25c, and itgb4 is expected to assess the prognosis of lac patients.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
The Protective Role of Brain CYP2J in Parkinson’s Disease Models

Cyp2j proteins are present in the neural cells of human and rodent brain regions.The aim of this study was to investigate the role of brain cyp2j in parkinson’s disease.Rats received right unilateral injection with lipopolysaccharide (lps) or 6‐hydroxydopamine (6‐ohda) in the substantia nigra following transfection with or without the cyp2j3 expression vector.Compared with lps‐treated rats, cyp2j3 transfection significantly decreased apomorphine‐induced rotation by 57.3% at day 12 and 47.0% at day 21 after lps treatment; moreover, cyp2j3 transfection attenuated the accumulation of α ‐synuclein.Compared with the 6‐ohda group, the number of rotations by rats transfected with cyp2j3 decreased by 59.6% at day 12 and 43.5% at day 21 after 6‐ohda treatment.The loss of dopaminergic neurons and the inhibition of the antioxidative system induced by lps or 6‐ohda were attenuated following cyp2j3 transfection.The tlr4‐my d88 signaling pathway was involved in the downregulation of brain cyp2j induced by lps, and cyp2j transfection upregulated the expression of nrf2 via the inhibition of mi r‐340 in u251 cells.The data suggest that increased levels of cyp2j in the brain can delay the pathological progression of pd initiated by inflammation or neurotoxins.The alteration of the metabolism of the endogenous substrates (e.g., aa) could affect the risk of neurodegenerative disease.

Journal: Oxidative Medicine and Cellular Longevity (2018)
Publication types: Research Article;
The Promotional Effect of Hollow MnO2 with Brucea Javanica Oil Emulsion (BJOE) on Endometrial Cancer Apoptosis

Endometrial cancer (ec) is a common gynecological malignancy worldwide whose therapy mainly depends on chemotherapy.In past years, an increasing number of studies indicate that hollow mn o 2 could serve as a nanoplatform in the drug delivery system.The brucea javanica oil emulsion (bjoe) has been illustrated to play a vital role in cancers.However, knowledge about the combined effect of h‐mn o 2 ‐peg/bjoe in endometrial cancer remains ambiguous up to now.In the present work, we prepared a drug‐delivery vector h‐mn o 2 ‐peg by chemical synthesis and found that h‐mn o 2 ‐peg significantly inhibited cell proliferation in endometrial cancer cells.Moreover, the combination of h‐mn o 2 ‐peg/bjoe could repress cell proliferation more efficiently and promote cell apoptosis.Mechanistically, we found that bjoe exerted its role as a promoter of endometrial apoptosis by regulating relative protein expressions.In general, the present study demonstrates that h‐mn o 2 ‐peg functions as a critical vector in the tumor microenvironment of endometrial cancer and the significant effect of h‐mn o 2 ‐peg/bjoe on cancer cells, suggesting a new paradigm for the treatment of endometrial cancer.

Journal: BioMed Research International (2021)
Publication types: Research Article;
The Optos 200Tx Scanning Laser Ophthalmoscope Application in Retinoblastoma Patients’ Follow-Up

And objective .Examination under general anesthesia is the conventional method of follow‐up examination for retinoblastoma (rb) patients.As most of the rb patients are infants or children, general anesthesia for examination often put the children at great risk and affect their growth and development.This retrospective study was aimed at observing and evaluating the application value of optos 200tx scanning laser ophthalmoscope (optomap 200tx) in the long‐term follow‐up of patients with rb who achieves stable disease after treatment.Methods .A total of 1134 examinations with optomap 200tx were performed for 318 children who were clinically diagnosed with rb in the ophthalmology department of tianjin medical university eye hospital, china, between july 2015 and july 2017, and achieved stable disease lasting for more than 6 months after combined treatment.The children received examinations every 1‐12 months (mean 4 months), initially at 31 months to 15 years of age (mean 51 months), and were given a full eye examination under anesthesia (eua) immediately if recurrent tumor, recurrent vitreous seeding (vs), or recurrent subretinal seeding (srs) was detected, or in the next follow‐up visit if no abnormality was detected, and early treatment was performed when the lesion was confirmed.Results .Recurrence was detected in 4 children in the examination with optomap 200tx, including 2 cases of recurrent vitreous seeding (vs) and 2 cases of recurrent subretinal seeding (srs), which were confirmed by eua and well controlled after early treatment.Conclusion .The use of optomap 200tx in the long‐term following up of patients with rb reduces the number of eye examinations under general anesthesia (eua), increases the time between euas, and protects children from exposure to the adverse effects of general anesthetics.Optomap 200tx can detect recurrent tumor and recurrent seeding, allowing for early treatment which produces better outcomes.

Journal: BioMed Research International (2022)
Publication types: Research Article;