syedzayyan/paper_mill_benchmark huggingface dataset:
Regulation of Tumorigenesis in Hepatocellular Carcinoma via the AKT3 Pathway in Cell Lines

The estimation of hepatocellular carcinoma (hcc) is tremendously inferior because of the formation of chemoresistance, integrated with essentially increased stemness belongings.At present, the relevance between mi r-122 and cancer development was mostly undisclosed with single study reflecting its importance in glioblastoma.Material and methods.The research here was focused to investigate the task of mi r-122 to modulate tumorigenesis in hepatocellular carcinoma by aiming akt3 in the management of hepatocellular carcinoma stemness and chemosensitivity.The method of qrt-pcr was performed to investigate the aspect of mi r-122 and akt3 in tissue sample and cell lines.The evaluation was done using gain- or loss-of-function in order to retrieve the function of mi r-122 in the hepatocellular carcinoma cells, including cell multiplication and stemness effects.The properties of hepatocellular carcinoma were discovered by the development of sphere development, cell feasibility, and the emergence of colony.Rna immunoprecipitation (rip), luciferase reporter, and the rna pull down evaluation were conducted to investigate the communication between the mi r-122 and akt3.Therefore, a naked mouse xenograft specimen was set up for the in vivo analysis.Results.Here, we determined the new role of akt3 and unmediated target of mi r-122.The reimposition of mi r-122 appearance in the hepatocellular carcinoma cell lines reduces the levels of akt3 and also hinders the relocation and expansion of cells by prompting apoptosis.These phenotypes are antitumor in nature and can be retrieved by the reorganization of the akt3 expression which signals the crucial role of akt3 in the mi r-122 arbitrated hcc modification.The in vivo analysis demonstrated the reimposition of mi r-122 entirely obstructing the xenograft expansion to manage tumorigenesis in hepatocellular carcinoma and a prospective remedial entrant for the liver cancer.Conclusion.In this study, it was observed that mi r-122 encourages the stemness role of hepatocellular carcinoma and diminishes the chemosensitivity by cleaning the mi r-122 in order to initiate the akt3.The in vivo study reflected the restoration of mi r-122 which completely hinders the xenograft growth to regulate the tumorigenesis in the hcc.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Research Article;
Reconstruction Algorithm-Based Ultrasonic and Spiral CT Images in Evaluating the Effects of Dexmedetomidine Anesthesia for Acute Abdomen

The study focused on the application value of iteration reconstruction algorithm-based ultrasound and spiral computed tomography (ct) examinations, and the safety of dexmedetomidine anesthesia in acute abdominal surgery.Methods.80 cases having the acute abdomen surgery were selected as the research subjects.They were divided into group a (40 cases) and group b (40 cases) according to the anesthetic drugs used in the later period.The experimental group was injected with propofol, remifentanil, and atracurium combined with dexmedetomidine; the control group was injected with propofol, remifentanil, and atracurium only.After the operation, the patient was for observed for the pain, agitation, adverse reactions, heart rate (hr), and blood pressure.All patients received ultrasound and spiral ct examinations, and based on the characteristics of the back-projection algorithm, an accelerated algorithm was established and used to process the image, and according to which, the patient’s condition and curative effects were evaluated.Results.After image reconstruction, the ultrasound and spiral ct images were clearer with less noise and more prominent lesions than before reconstruction.Before image reconstruction, the accuracy rates of ultrasound and spiral ct in diagnosing acute abdomen were 92.3% and 91.1%, respectively.After reconstruction, the corresponding numbers were 96.3% and 98.1%, respectively.After reconstruction, the accuracy of the two methods in diagnosing acute abdomen was significantly improved compared with that before reconstruction, and the difference was statistically significant ( p &lt; 0.05 ).The ramsay score of the experimental group was significantly higher than that of the control group at each time period, p &lt; 0.05 ; the agitation score and visual analogue scale (vas) score of the experimental group were significantly lower than the control group at each time period after waking up, p &lt; 0.05 .Conclusion.Reconstruction algorithm-based ultrasound and spiral ct images have high application value in the diagnosis of patients with acute abdomen, and dexmedetomidine has good safety in anesthesia surgery.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Clinical Study;
Recognition of the Possible miRNA-mRNA Controlling Network in Stroke by Bioinformatics Examination

Based on the latest research of who, it has been revealed that more than 15 million people suffer from stroke every year worldwide.Of these 15 million people, 6 million succumb to death, and 5 million get permanently disabled.This is the prime reason for the substantial economic burden on all parts of the world.Methods.These data have been obtained from the geo database, and the geo2r tool was used to find out the differentially expressed mi rnas (dems) between the stroke and normal patients’ blood.Fun rich and mi rnet were considered to find potential upstream transcription factors and downstream target genes of candidate emrs.Next, we use go annotation and kegg pathway enrichment.Target genes were analyzed with the help of the r software.Then, the string database and cytoscape software were used to conduct ppi and dem-hub gene networks.Finally, gse58294 was used to estimate the hub gene expressions.Results.Six dems in total were selected out from gse95204 and gse117064 datasets.663 dems’ target genes were predicted, and nrf1, egr1, myc, yy1, e2f1, sp4, and sp1 were predicted as an upstream transcription factor for dems’ target genes.Target genes of dems were primarily augmented in the pi3k-akt signaling pathway and p53 signaling pathway.The network construction of dem hygiene is potentially modulated by hsa-mi r-3591-5p, hsa-mi r-548as-3p, hsa-mi r-206, and hsa-mi r-4503 hub genes which were found among the top 10 of the hub genes.Among the top 10 hub genes, justification of ctnnb1, pten, esr1, ccnd1, kras, akt1, ccnd2, cdkn1b, and mycn was constant with that in the gse58294 dataset.Conclusion.In summary, our research first constructs the mi rna-m rna network in stroke, which probably renders an awakening purview into the pathogenesis and cure of stroke.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Research Article;
Quality Evaluation of Ranunculaceae Essential Oil with the Artificial Neural Network

The goal of this project is to use ann to build a quality evaluation method for ranunculaceae essential oils in order to assist businesses or individuals in setting up an automated essential oil quality evaluation system and reducing human resource consumption.Using computer software to evaluate the quality of ranunculaceae essential oils, quantitatively evaluate product quality, and improve the automation of quality evaluation.The main research contents in this paper are as follows: (1) in-depth research on pso and its development.The purpose of studying the pso is to use pso to replace gradient descent algorithm in bp network, so that the neural network model has a better network structure.This paper improves it on the basis of pso.The basic improvement idea is to add a dynamic random position transformation to the particle, so that global optimization ability can be enhanced.The improved algorithm and the standard algorithm are compared and analyzed in the simulation experiments.The results verify that improved pso has better optimization ability.(2) replace training algorithm in the bp network with the ipso and construct the bp network algorithm model of ipso.The final implementation of the ranunculaceae plant essential oil quality assessment model based on the ipso-bp network was achieved by combining the ranunculaceae plant essential oil quality evaluation index system with the ranunculaceae plant essential oil quality evaluation index system.Finally, a simulation experiment was designed to compare the presentation of pso-bp and ipso-bp, when they were applied to the evaluation of ranunculaceae vital oils.The experimental results show ipso-bp has the highest estimate accuracy and the best performance.

Journal: Security and Communication Networks (2022)
Publication types: Research Article;
Protumor Effects of Histone H3-H4 Chaperone Antisilencing Feature 1B Gene on Lung Adenocarcinoma: In Silico and In Vitro Analyses

Asf1b is a member of the histone h3–h4 chaperone antisilencing feature 1 (asf1).Asf1b reportedly acts as an oncogene in several cancers including, breast cancer and cervical cancer.To date, the role of asf1b in lung adenocarcinoma (luad) is not elucidated.Methods.The tcga database, containing data for 33 cancer types, was used to explore the dysregulation and prognostic value of the asf1b gene in pan-cancer data.R software packages and public databases/webservers were applied for bioinformatics and statistical analyses.Using in vitro models, immunoprecipitation and immunofluorescence were utilized to investigate if bcar1 interacted with asf1b in luad.Further, transfection experiments were performed to validate the expression pattern of asf1b in luad and examine its regulating role in tumor-associated processes including tumor cell proliferation and migration.Results.Asf1b was found to be significantly elevated in luad and the majority of cancer types, except pcpg (pheochromocytoma and paraganglioma).The overexpression of asf1b was associated with worse prognostic outcomes in most cancer types including luad.Asf1b was associated with lymph node metastasis, and in vitro, it promoted the proliferation and migration of luad cells.Asf1b knockdown suppressed luad cell proliferation and migration and also diminished the expression of cell cycle, metastasis, and emt signaling-associated proteins.Bcar1 was found positively correlated and interacting with asf1b, and bcar1 overexpression reversed the effects of asf1b knockdown in luad cells.Conclusion.These findings indicated that asf1b plays a significant role in the tumor progression of luad and bcar1 mediates the tumor-promotive effects of asf1b, acting as an intermediate protein.Therefore, the asf1b/bcar1 axis might be regarded as a putative therapeutic target for luad.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Research Article;
Protective Properties of the Extract of Chrysanthemum on Patients with Ischemic Stroke

Investigation of the protective effect of chrysanthemum extract in ischemic strokes patients is among the challenging issues with the traditional hospital system in general and smart technology-based hospitals in particular.In this study, we have evaluated the protective effect of chrysanthemum extract on patients with ischemic stroke by detecting the severity of stroke, neuronal indexes, and oxidative stress biomarkers.For this purpose, forty-six patients with ischemic stroke were randomly divided into the control group (n = 30) and chrysanthemum group (n = 30).The control group received standard stroke treatment, and the chrysanthemum group was treated with chrysanthemum extract 400 mg/day (200 mg/day, twice/day) on the basis of standard treatment.The groups were compared the effect of saffron capsules using the national institute of health stoke scale (nihss), serum neuron specific enolase (nse), s100, brain-derived neurotrophic factor (bdnf), malondialdehyde (mda), su-peroxide dismutase (sod), and total antioxidant capacity (tac ) levels, at the time of first day and fourth day after treatment.On the first day after treatment, there was no significant difference in the nihss score, serum nse, s100, bdnf, mda, sod, and tac levels between the chrysanthemum group and the control group ( p &gt; 0.05 ).On the fourth day after treatment, the nihss, serum nse, s100, and mda levels were significantly reduced in the chrysanthemum group compared to the control group, while the bdnf, sod, and tac levels were higher ( p &lt; 0.05 ).In addition, compared to the levels on the first day, the nihss, serum nse, s100, and mda levels were significantly reduced, and the bdnf, sod, and tac levels were increased in the chrysanthemum group on the fourth day ( p &lt; 0.05 ).Chrysanthemum extract has the effects of scavenging oxygen free radicals and antioxidation and has a neuroprotective effect on ischemic stroke patients.

Journal: Journal of Healthcare Engineering (2021)
Publication types: Clinical Study;
Protective Effect and Mechanism of Traditional Chinese Medicine on Myocardial Ischemia Reperfusion Injury

After acute myocardial infarction, early restoration of myocardial perfusion by thrombolysis or percutaneous coronary intervention is the most effective way to reduce the size of myocardial infarction and improve clinical outcomes.However, recovery of blood flow to the ischemic myocardium may cause ischemia-reperfusion (i/r) injury, a phenomenon that instead reduces the efficacy of myocardial reperfusion.Traditional chinese medicine (tcm) has long been used for the treatment of cardiovascular diseases and has shown remarkable efficacy.Many studies have shown that some tcms and their active components can exert protective effects against myocardial i/r injury through different mechanisms.This review summarized the protective mechanisms and current research advances of tcms in myocardial i/r injury.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Review Article;
Prognostic Factors of IIIAN2 Non-Small-Cell Lung Cancer after Complete Resection: A Systemic Review and Meta-analysis

And background.We designed this systemic review meta-analysis of all the reported scientific literature to conclude the prognostic factors of iiian2 non-small-cell lung cancer after complete surgical resection.Management of nsclc iiian2 involved different strategies, such as complete resection, chemotherapy, radiotherapy, chemoradiotherapy, and induction therapy.Each management strategy has its associated prognostic factor to monitor for better patient prognosis, recovery, survival rate, and minimize the chances of recurrence.Methods.An extensive data search was conducted from all leading databases including pub med, google scholar, embase, and cochrane.Fifteen studies were selected according to the prisma model of data selected to conduct this systemic review meta-analysis.Results.Total 4444 patients were evaluated among fifteen selected studies.A number of lymph nodes involved ( n = 3965 ), level of lymph nodes ( n = 3422 ), and complete tumor resection ( n = 3255 ) were the most reported prognostic factors.Conclusion.This study exhibits the overall significance of all prognostic factors of nsclc iiian2 pathology for better patient management.However, other management strategies also play a significant contribution to achieving a better survival rate and less recurrence possibility.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Meta-Analysis;
Prognostic Factor Study of Macular Edema Recurrence in Retinal Vein Occlusion after Conbercept Treatment: A Post Hoc Analysis of the FALCON Study

The study was aimed at exploring the potential predictive factors associated with the recurrence of macular edema (me) secondary to vein occlusion (rvo) after intravitreal antivascular endothelial growth factor (vegf) loading treatment in the falcon study.Methods.This is a post hoc analysis of 30 patients with central rvo and 30 patients with branch rvo.All patients received a monthly administration of intravitreal conbercept during the 3-month loading phase and pro re nata (prn) treatment during the 6-month follow-up period.Based on the recurrence of me at the first follow-up visit, patients were classified into the recurrence group or nonrecurrence group.The primary endpoint was to explore the risk factors for recurrence among baseline characteristics, fluorescein angiography (fa) patterns, and optical coherence tomography (oct).Results.In general, 38 patients (64.4%) experienced me recurrence at the first follow-up visit (3 months), regardless of disease type ( p = 0.32 ).Significant improvements in va were noted in both the nonrecurrence and recurrence groups ( p &lt; 0.001 ), however, without significant between-group differences ( p = 0.1 ).A significant reduction in crt in both groups ( p &lt; 0.001 ) was identified, and patients without recurrence showed a greater reduction in crt compared with those with recurrence ( p &lt; 0.001 ).In addition, logistic regression analyses indicated the corrections of me recurrence with baseline macular volume and the disruption of the outer limiting membrane at the fovea.Conclusion.This study suggested that oct parameters, including baseline macular volume and outer limiting membrane disruption, and reduction in crt after loading therapy were more predictive of me recurrence than fa patterns or visual changes following conbercept loading therapy.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
Problems and Countermeasures of China’s International Trade in Agricultural Products under the Belt and Road Strategy Based on Big Data Analysis Technology in the Internet of Things Era

In order to study the analysis of china’s trade development under big data technology, through the analysis of the import and export of trade, the expansion of international trade exports of agricultural products, the adjustment of agricultural structure, the enrichment of import and export markets, the security of agricultural products, and other issues through the internet of things technology and traditional technology, as well as the comparison of the overall effect, comprehensive performance, and coupling data, it can be seen that the agricultural trade under internet of things technology is more convenient and faster.The application of network technology makes things better connected with people, changes the previous way of life, speeds up the development process of international trade, and realizes the real intelligent era.

Journal: Security and Communication Networks (2022)
Publication types: Research Article;
Preparation of Iron Oxide and Titania-Based Composite, Core-Shell Populated, Nanoparticulates Material by Two-Step LASER Ablation in Aqueous Media as Antimicrobial and Anticancer Agents

Iron oxide and titania‐based composite nanoparticles (nps) populated with core‐shell structures, as part of the mixture of the monometallic nps, were prepared in water medium by the two‐fluence laser ablation technique by applying 30 and 60 m j/cm 2 laser energy irradiations.The prepared monometallics, composite, and core‐shell nps structures were confirmed from the xrd, tem, and edx analyses, followed by the fe‐sem and uv absorptions.Optically, the nps exhibited an increase in the energy gap from 3.27 e v to 3.75 e v as laser fluence increased from 30 m j/cm 2 to 60 m j/cm 2 .The average nps core size distributions for the core‐shell material ranged at ∼70 nm with the shell thickness around 20 nm.The biggest nps were of ∼170 nm size which were sparsely distributed.The magnetization behaviors of the nps were also investigated using the vibrating sample magnetometer (vsm).The nps showed antimicrobial activities against the pathogenic species: escherichia coli and staphylococcus aureus .The antimicrobial activities of the synthesized nps, synthesized under the influence of magnetic fields, were found to be more potent than the nps synthesized without the presence of any magnetic field.The nps prepared under the influence of the magnetic fields also comparatively exhibited higher levels of cytotoxicity against lung cancer cell lines (a549) than the nps prepared under no magnetic field’s influence by the similar energy level effects of the laser fluence.The flow cytometry analyses confirmed the nps’ cytotoxic impacts against the human lung cancer a549 cell lines through the initiation of apoptosis and promotion of the cell cycle arrest at the g1 phase of cell division.To further confirm the cytotoxic effects and the mechanism of the anticancer activity of the synthesized nps against the a549 cell lines, several related parameters (cell viability, membrane permeability, nuclear intensity, and cytochrome‐c release) were analyzed using the high‐content screening (hcs) assay.The study suggested that the prepared nps have potential as antimicrobial and also as anti‐lung‐cancer agents as tested in vitro .These nps can also be part of combined chemotherapy in different oncological interventions, as well as a sonosensitizer in sonomagnetic heating‐based therapy, especially for cancers.

Journal: Bioinorganic Chemistry and Applications (2022)
Publication types: Research Article;
Preliminary Study on Risk Factors for Morbidity of Nonalcoholic Fatty Liver Disease in High-Income Male Population

S.Believed to be a result of metabolic syndrome and unhealthy lifestyle, the incidence of nonalcoholic fatty liver disease (nafld) has become a serious public health problem.Among the high-income male population, metabolic syndrome and unhealthy lifestyle are particularly prominent.Therefore, we conducted a survey on 375 high-income male subjects, expecting to understand the risk factors and related factors for morbidity of nafld among the high-income male population being physically examined in shanghai.Methods.A cross-sectional study was applied to 375 high-income male subjects (including 190 patients with nafld and 185 non-nafld subjects) who were examined in the special needs clinic at huadong hospital affiliated to fudan university.In combination with medical history, physical examination, and laboratory test results and by use of a self-made nafld health questionnaire, the basic data of the research objects were collected and the obtained data were subject to a correlation analysis.Results.This study investigated 375 high-income males, and the morbidity rate of nafld was 50.67%.The nafld group was higher than the non-nafld group in terms of body weight, bmi, systolic blood pressure, and diastolic blood pressure ( p &lt; 0.05 ).Hypertension (or = 2.944), diabetes (or = 7.278), and hyperuricemia (or = 1.922) are the risk factors for nafld; compared with no metabolic diseases, one (or = 1.848), two (or = 2.417), and three metabolic diseases (or = 14.788) are risk factors for the development of nafld.Compared with the non-nafld group, the nafld group had a higher level of wbc, rbc, hb, plt, fpg, hb a1c, alt, ast, ggt, alp, tp, and ua ( p &lt; 0.05 ).There was a statistically significant difference in the intake of supper and staple foods between the nafld group and the non-nafld group, and the highly greasy diet was a risk factor for nafld (or = 2.173) as opposed to the nongreasy diet.Conclusion.High-income male population is a high-risk group of nafld.Most of the patients with nafld have abnormal biochemical indicators as opposed to the healthy population and are more likely to be complicated with other chronic diseases or abnormal health status.And the occurrence of hypertension, diabetes, and hyperuricemia is the risk factor for the development of nafld.At the same time, the number of metabolic diseases complicated is also a risk factor for nafld as compared with the absence of complications with such metabolic diseases.Compared with a diet that is not greasy, the fact that high-income male nafld patients have a very greasy diet increases the risk of nafld.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;