syedzayyan/paper_mill_benchmark huggingface dataset:
Partial glycosylation of the Ibaraki virus NS3 protein is sufficient to support virus propagation.

Ibaraki virus (ibav) causes ibaraki disease.We have previously shown that ibav ns3 protein is highly glycosylated and that tunicamycin, an inhibitor of n-linked glycosylation, suppressed ns3 glycosylation and viral propagation.Since tunicamycin is known to cause endoplasmic reticulum (er) stress, we explored the effects of er stress and ns3 glycosylation on ibav infection using tunicamycin and thapsigargin.These reagents both induced er stress and ns3 glycosylation inhibition in a concentration-dependent manner, and as in our previous report, high concentrations of tunicamycin and thapsigargin suppressed ibav propagation.However, lower concentrations of these reagents produced limited differences in ibav propagation, despite their ability to suppress ns3 glycosylation and induce er stress.These findings suggest that a considerable degree of ns3 glycosylation inhibition and er stress induction does not suppress ibav propagation.Conversely, lower concentrations of thapsigargin enhanced ibav propagation, suggesting that moderate er stress could benefit ibav.

Journal: Virology (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Calcium Fructoborate Prevents Skin Cancer Development in Balb-c Mice: Next Part, Reverse Inflammation, and Metabolic Alteration.

Metabolic alterations and inflammation are regarded as hallmarks of cancer.Glycolytic flux and intermediate accumulation lead to the production of building blocks and nadph which is important in protecting the cell from oxidative damage.Inflammation causes the release of mediators responsible for regulating molecular mechanism affecting metabolic pathways.Ca fb due to its cis-diol-rich feature may have the potential to interact with molecules taking part in cancer development.This study was aimed to investigate the effects of ca fb on metabolic alterations and inflammation in 7,12-dimethylbenz(a)anthracene (dmba)/12-o-tetradecanoylphorbol-13-acetate (tpa)-induced skin cancer.For this purpose, 92 balb-c mice were distributed into 6 groups as control, ca fb, dmba/tpa (d-t), treatment 1 (t1), 2 (t2), and 3(t3).Apart from control and ca fb in other groups, tumors initiated with 97.5-nmol dmba and 6.5-nmol tpa.Treatment groups received 3 mg/kg/day ca fb with dmba (t1), with tpa (t2), and after tumor formation (t3).In the d-t group, glyceraldehyde-3-phosphate dehydrogenase (gapdh) activity, 6-phosphogluconate dehydrogenase (pgd), glutathione (gsh), interleukin 6 (il-6), (il-1β), tumor necrosis factor-α (tnf-α) levels increased (p < 0.001) while malondialdehyde (mda) levels decreased (p < 0.001) compared with that in control.Ca fb application ameliorated dmba-tpa effect according to the distribution time.It is noteworthy to consider ca fb as a potential preventive agent in skin cancer development.

Journal: Biological trace element research (2021)
Publication types: Journal Article
Impact of Sociodemographic Features and Lifestyle on Cognitive Performance of Peruvian Adults.

Cognitive impairment and dementia may result from a combination of modifiable and nonmodifiable risk and protective factors, such as the environment, educational attainment, time devoted to cognitively stimulating activities, and physical activity.Objective this study aimed to investigate the mediating role of sociodemographic characteristics and lifestyle factors in the years of education and cognitive performance in peruvian adults.Methods this cross-sectional study included 1,478 subjects assessed by addenbrooke's cognitive examination revised (ace-r).Using mediation models, we evaluated the mediation role of parents' educational level, reading time (rt), and physical activity time (pat) in the years of education (iye) and cognitive performance.Results people who reported having lived in an urban area during their childhood are estimated to have, on average, 2.085 years more formal education than those who lived in rural areas.In addition, 49% of cognitive performance scores are explained by the mediation effect of reading and physical activity time in the iye.This implies that higher levels of education, mediated by rt and pat per week, are 1.596 units associated with higher scores on the ace-r.Conclusion despite the fact that nonmodifiable factors (i.e., childhood residence area, parents' educational level) seem to exert an effect on older adults' cognition, their influence is mediated by other factors that are indeed modifiable (i.e., reading time, physical activity engagement).In this sense, lifestyle changes could help prevent or decrease the risk of cognitive impairment and reduce the disease's impact on vulnerable environments in latin american and caribbean countries.

Journal: Journal of Alzheimer's disease : JAD (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
An Estimation Model of Urban Land Accessibility.

Given that there are no practical quantitative indicators of traffic conditions for facility location selection in the process of urbanization, this article proposes a comprehensive accessibility index of location and its measurement method.Urban land is rasterized using gis to obtain the grids, and the road network data are used to calculate the external accessibility and internal accessibility of the grids.The external accessibility and the internal accessibility of a grid are combined to obtain the comprehensive accessibility of the location.The comprehensive accessibilities of grids are measured for zhicheng, an urban area in china.The results show that the pattern of gradual spatial changes in the comprehensive accessibility of the grids in zhicheng is highly consistent with the urban land's spatial development trend, which verifies the feasibility and accuracy of the comprehensive accessibility measurement method.On one hand, the comprehensive accessibility of the grid is more portable than the accessibility of a single point and can be calculated in batches.On the other hand, it is more specific than the regional accessibility and better guides the location selection of urban facilities.

Journal: International journal of environmental research and public health (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Effects of Adherence to Once-Daily Treatment on Lung Function, Bronchial Hyperreactivity and Health Outcomes in Adolescents with Mild-to-Moderate Asthmoka: A Twelve-Month Survey.

Adolescents with asthma are usually insufficiently adherent to regular inhalation treatments, thus limiting their effectiveness.The aim of this study is to investigate the role of adherence to single-inhaler long-acting laba/ics dry-powder combination o.d.In affecting lung function, bronchial hyperreactivity, and health outcomes over a twelve-month survey of a group of non-smoking adolescents with mild to moderate asthma.Methods: age, gender, bmi and atopy, forced expiratory volume in 1 s (fev1), maximum mid-expiratory flow (mmef), and maximum expiratory flow at 25% of lung filling (mef25) were assessed via a boolean selection process from the institutional database at recruitment, as well as after 6 and 12 months, together with the incidence of exacerbation, school days that were taken off, gp and specialist visits, and systemic steroid and/or antibiotic courses.Adherence was checked monthly via a direct telephone call.Statistics were calculated with an anova trend analysis, assuming p < 0.05.Results: two well-matched sample groups of 54 subjects each were obtained.The mean annual adherence to treatment ranged from 48.2% doses ± 10.9 sd to 79.3% doses ± 8.8 sd (p < 0.001), regardless of age and gender.Only adolescents that adhered to the o.d.Ics/laba inhalation regimen progressively achieved complete control of all lung function parameters (fev1: 0.001; mmef: p < 0.002; mef25 < 0.001; <0.001), minimized their bronchial hyperreactivity (p < 0.001), and optimized all health outcomes (p < 0.001—p < 0.002) over the survey duration.Discussion: a good adherence to treatment is essential for asthma management, particularly in young patients.Factors that are totally independent of the complexity of the therapeutic regimen adopted (namely, only a once-daily inhalation in the present survey) probably represent the major reasons limiting the adolescents’ adherence.Cultural, educational, behavioral, and psychological factors are frequently involved, are difficult to control, and can present barriers to adolescents’ asthma management.Further studies aiming to deeply understand and possibly remove the reasons for such adolescents’ attitudes are needed, in cooperation with actions oriented in this direction by families, educators, and health professionals.

Journal: Children (Basel, Switzerland) (2022)
Publication types: Journal Article
In vitro activity of ceragenins against Burkholderia cepacia complex.

Burkholderia cepacia complex (bcc) species are aerobic, gram-negative and non-fermantative bacilli.Bcc can cause clinical symptoms in patients with cystic fibrosis, ranging from asymptomatic carriage to fatal pneumonia.A pressing need exists for new antimicrobial agents that target bcc.Ceragenins, csa-13, csa-131 and csa-131 with 5% pluronic® f127 (csa-131p), were evaluated against bcc clinical isolates (n = 42).Mics of ceragenins and conventional antibiotics were determined.Time-kill curve experiments were performed with 1x, 4x mics of ceragenins and sulfamethoxazole-trimethoprim (sxt), levofloxacin.Mic50/ mic90results (mg l-1) of csa-13, csa-131 and csa-131p were determined as 16/64, 16/128 and 16/128, respectively.Csa-13 and csa-131 showed bactericidal activity.Csa-13 - levofloxacin combination displayed synergistic activity against bcc.First-generation (csa-13) and second-generation (csa-131 and csa-131p) ceragenins have significant antimicrobial effects on bcc.The findings of this study demonstrate that combinations of ceragenins with currently marketed antibiotics could be synergistic in vitro against bcc isolates.These results suggest that combination therapy with conventional antibiotics could be an alternative approach for treating bcc infections in the future.

Journal: The Journal of antibiotics (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Survival after laparoscopy versus laparotomy for apparent early-stage uterine clear cell carcinoma: Results of a large multicenter cohort study.

To compare the long-term survival between laparoscopic surgery and open surgery in patients with apparent early-stage uterine clear cell carcinoma (uccc).Patients and methods 254 patients with apparent early-stage uccc were reviewed.Comparisons were made between patients who underwent laparoscopic surgery versus those who underwent open surgery.Baseline data, clinicopathological data, and oncological outcomes were analyzed.5-year disease-free survival (dfs) rate and 5-year overall survival (os) rate were estimated and compared using the kaplan-meier method and the log-rank test.The cox proportional hazard regression model was employed to control the confounding factors.Results 147 patients underwent laparoscopic surgery, and 107 patients were managed by open surgery.No differences in terms of recurrence rate (laparoscopy versus laparotomy: 10.9% versus 12.9%,p=0.842) and recurrence pattern were observed.For patients who underwent open surgery and patients who underwent laparoscopic surgery, the 5-year dfs rates and 5-year os rate were 75.8% (95% ci: 65.8%-83.2%) and 69.1% (95% ci: 58.8%-77.4%), 66.0% (95% ci: 57.1%-73.5%) and 60.8% (95% ci: 52.0%-68.5%), respectively.The cox proportional hazards regression model shown that for apparent early-stage uccc, the approach of surgical staging was not an independent predictor for survival (laparoscopy versus laparotomy: for dfs, a hr=1.06, 95% ci=0.64-1.75,p=0.826; for os, a hr=1.10, 95% ci=0.72-1.68,p=0.671).Conclusion for apparent early-stage uccc, in terms of oncological survival, laparoscopic surgery was as safe as open surgery.

Journal: Frontiers in oncology (2022)
Publication types: Journal Article
Change in country-level COVID-19 lethality is associated with improved testing: no apparent role of medical care or disease-specific knowledge.

Reductions in the case fatality rate of covid-19 in the unvaccinated have been credited to improvements in medical care.Here i test whether either of these factors predicts reductions in the case fatality rate, and whether observed reductions are better explicable by improved ascertainment of mild cases.Methods using weighted log-log regression, i compute the association between changes in the case fatality rate and test density between 3 july 2020 and 5 january 2021 in 162 countries; and check whether case fatality rate change is associated with either per capita medical spending (proxy for critical care access) or timing of the pandemic (proxy for covid-specific knowledge).Results the median test density increased from 175 tests per thousand population to 1200, while the median case fatality rate dropped from 4.1% to 2.0%.While the case fatality rate was higher at both timepoints in europe/north america than africa / asia, its association with test density was similar across countries.For each doubling in test density, the mean case fatality rate decreased by 18% (p 0.10).Conclusions declines in the case fatality rate were adequately explained by improved testing, with no effect of either medical knowledge or improvements in care.The true lethality of covid-19 may not have changed much at the population level.Prevention should remain a priority.

Journal: Scandinavian journal of public health (2022)
Publication types: Journal Article
Transcriptome Analysis Reveals the Stress Tolerance to and Accumulation Mechanisms of Cadmium inPaspalum vaginatumSwartz.

Cadmium (cd) is a non-essential heavy metal and high concentrations in plants causes toxicity of their edible parts and acts as a carcinogen to humans and animals.paspalum vaginatumis widely cultivating as turfgrass due to its higher abiotic stress tolerance ability.However, there is no clear evidence to elucidate the mechanism for heavy metal tolerance, including cd.In this study, an rna sequencing technique was employed to investigate the key genes associated with cd stress tolerance and accumulation in p.Vaginatum.The results revealed that antioxidant enzyme activities catalase (cat), peroxidase (pod), superoxide dismutase (sod), and glutathione s-transferase gst) were significantly higher at 24 h than in other treatments.A total of 6820 (4457/2363, up-/down-regulated), 14,038 (9894/4144, up-/down-regulated) and 17,327 (7956/9371, up-/down-regulated) differentially expressed genes (degs) between the cd1 vs.Cd0, cd4 vs.Cd0, and cd24 vs.Cd0, respectively, were identified.The go analysis and the kegg pathway enrichment analysis showed that degs participated in many significant pathways in response to cd stress.The response to abiotic stimulus, the metal transport mechanism, glutathione metabolism, and the consistency of transcription factor activity were among the most enriched pathways.The validation of gene expression by q rt-pcr results showed that heavy metal transporters and signaling response genes were significantly enriched with increasing sampling intervals, presenting consistency to the transcriptome data.Furthermore, over-expression of pv sn rk2.7can positively regulate cd-tolerance in arabidopsis.In conclusion, our results provided a novel molecular mechanism of the cd stress tolerance of p.Vaginatumand will lay the foundation for target breeding of cd tolerance in turfgrass.

Journal: Plants (Basel, Switzerland) (2022)
Publication types: Journal Article
Current knowledge about biomarkers of acute kidney injury in liver cirrhosis.

Acute kidney injury (aki) is common in advanced cirrhosis.Prerenal azotemia, hepatorenal syndrome, and acute tubular necrosis are the main causes of aki in patients with cirrhosis.Evaluation of renal function and differentiation between functional and structural kidney injury are important issues in the management of cirrhosis.However, aki in cirrhosis exists as a complex clinical spectrum rather than concrete clinical entity.Based on current evidence, changes in serum creatinine (cr) levels remain the most appropriate standard for defining aki in cirrhosis.However, serum cr has a limited role in assessing renal function in this population.This review examines previous studies that investigated the ability of recent biomarkers for aki in cirrhosis from the perspective of earlier and accurate diagnosis, classification of aki phenotype, and prediction of clinical outcomes.Serum cystatin c and urine neutrophil gelatinase-associated lipocalin have been extensively studied in cirrhosis, and have facilitated improved diagnosis and prognosis prediction in patients with aki.In addition, urine n-acetyl-β-d-glucosaminidase, interleukin 18, and kidney injury molecule 1 are other promising biomarkers for advanced cirrhosis.However, the clinical significance of these markers remains unclear because there are no cut-off values defining the normal range and differentiating phenotypes of aki.In addition, aki has been defined in terms of serum cr, and renal biopsy-the gold standard-has not been carried out in most studies.Further discovery of innovate biomarkers and incorporation of various markers could improve the diagnosis and prognosis prediction of aki, and will translate into meaningful improvements in patient outcomes.

Journal: Clinical and molecular hepatology (2022)
Publication types: Journal Article;Review
Use of protective behavioral strategies and blackout experience among mandated college students.

Alcohol-induced blackouts are experienced by approximately half of college students who drink.People who use protective behavioral strategies (pbs) tend to experience fewer alcohol-related consequences of drinking, but the relationship between pbs and blackouts is unknown.This study examines the associations among the use of protective behavioral strategies and blacking out.Methods participants were 484 college students, aged 18-22 (56% male, 51% first-year), who were mandated to complete alcohol education following an alcohol violation.Before the intervention, participants completed questionnaires that included questions about past month peak consumption, alcohol-related consequences, marijuana use frequency, and frequency of pbs use.Logistic regression analyses were conducted to evaluate how use of pbs was related to the odds of experiencing a blackout, controlling for consumption and other risk factors.Results participants endorsing greater overall use of pbs had decreased odds of having experienced a blackout.However, this association depended on the type of pbs being used.Participants endorsing greater use of pbs regarding manner of drinking and stopping/limiting drinking had decreased odds of having experienced a blackout, but those who endorsed greater use of pbs relating to serious harm reduction did not.Conclusions this study adds nuance to the idea that pbs protect against adverse alcohol-related consequences.Only pbs that serve to limit or slow consumption appear to be protective against experiencing blackouts.Research on specific associations between types of pbs and consequences may lead to improved outcomes in interventions that incorporate pbs.

Journal: Addictive behaviors (2022)
Publication types: Journal Article
Control of Crystallinity and Stereocomplexation of Synthetic Carbohydrate Polymers from d- and l-Xylose.

Manipulating the stereochemistry of polymers is a powerful method to alter their physical properties.Despite the chirality of monosaccharides, reports on the impact of stereochemistry in natural polysaccharides and synthetic carbohydrate polymers remain absent.Herein, we report the cocrystallisation of regio- and stereoregular polyethers derived from d- and l-xylose, leading to enhanced thermal properties compared to the enantiopure polymers.To the best of our knowledge, this is the first example of a stereocomplex between carbohydrate polymers of opposite chirality.In contrast, atactic polymers obtained from a racemic mixture of monomers are amorphous.We also show that the polymer hydroxyl groups are amenable to post-polymerisation functionalization.These strategies afford a family of carbohydrate polyethers, the physical and chemical properties of which can both be controlled, and which opens new possibilities for polysaccharide mimics in biomedical applications or as advanced materials.

Journal: Angewandte Chemie (International ed. in English) (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
S100A16 promotes acute kidney injury by activating HRD1-induced ubiquitination and degradation of GSK3β and CK1α.

The pathogenesis of acute kidney injury (aki) is associated with the activation of multiple signaling pathways, including wnt/β-catenin signaling.However, the mechanism of wnt/β-catenin pathway activation in renal interstitial fibroblasts during aki is unclear.S100 calcium-binding protein a16 (s100a16), a new member of calcium-binding protein s100 family, is a multi-functional signaling factor involved in various pathogenies, including tumors, glycolipid metabolism disorder, and chronic kidney disease (ckd).We investigated the potential participation of s100a16 in wnt/β-catenin pathway activation during aki by subjecting wild-type (wt) and s100a16 knockout (s100a16+/-) mice to the ischemia-reperfusion injury (iri), and revealed s100a16 upregulation in this model, in which knockout of s100a16 impeded the wnt/β-catenin signaling pathway activation and recovered the expression of downstream hepatocyte growth factor (hgf).We also found that s100a16 was highly expressed in platelet-derived growth factor receptor beta (pdgfrβ) positive renal fibroblasts in vivo.Consistently, in rat renal interstitial fibroblasts (nrk-49f cells), both hypoxia/reoxygenation and s100a16 overexpression exacerbated fibroblasts apoptosis and inhibited hgf secretion; whereas s100a16 knockdown or wnt/β-catenin pathway inhibitor icg-001 reversed these changes.Mechanistically, we showed that s100a16 promoted wnt/β-catenin signaling activation via the ubiquitylation and degradation of β-catenin complex members, glycogen synthase kinase 3β (gsk3β) and casein kinase 1α (ck1α), mediated by e3 ubiquitin ligase, the hmg-co a reductase degradation protein 1 (hrd1).Our study identified the s100a16 as a key regulator in the activation of wnt/β-catenin signaling pathway in aki.

Journal: Cellular and molecular life sciences : CMLS (2022)
Publication types: Journal Article