syedzayyan/paper_mill_benchmark huggingface dataset:
Use of a novel digital drainage system after pulmonary resection.

The thoraguard surgical drainage system is a novel device for drainage of air and fluid after cardiothoracic surgery.Methods a three-part study was conducted: a prospective observational safety and feasibility study, a retrospective comparison of patients managed with an analogue drainage system, and a clinician user-feedback survey.Results fifty patients underwent robotic pulmonary resection utilizing the thoraguard system for postoperative drainage.The thoraguard system detected a higher number of air leaks than an analogue system (36/50, 72%vs.45/200, 23%; p<0.001) and was associated with decreased chest tube duration of 1 day [interquartile range (iqr) 0-2]vs.2 days (iqr 2-3) (p=0.042) and hospital length of stay of 2 days (iqr 2-3)vs.3 days (iqr 2-4) (p=0.027).Patients with a peak air leak less than 100 m l/min (32 patients, 64%), had a decreased median chest tube duration of 1 day (iqr 0-1)vs.2.8 days (iqr 1-3) (p=0.004).Compared to an analogue system, the thoraguard system had superior user-reported ability to detect air-leaks (17/23, 74%), better ease of patient ambulation (14/23, 61%), and better display of clinically relevant information (22/23, 96%).Conclusions the thoraguard surgical drainage system provides safe and effective drainage post pulmonary resection.Compared to an analogue system, the thoraguard system detected a higher number of air leaks and was associated with decreased chest tube duration and hospital length of stay.User survey data reported superior air leak detection, display of clinical data, and ease of use of the thoraguard system.

Journal: Journal of thoracic disease (2022)
Publication types: Journal Article
B cell overexpression of FCRL5 and PD-1 is associated with low antibody titers in HCV infection.

Antibodies targeting the hepatitis c virus (hcv) envelope glycoprotein e2 are associated with delayed disease progression, and these antibodies can also facilitate spontaneous clearance of infection in some individuals.However, many infected people demonstrate low titer and delayed anti-e2 antibody responses.Since a goal of hcv vaccine development is induction of high titers of anti-e2 antibodies, it is important to define the mechanisms underlying these suboptimal antibody responses.By staining lymphocytes with a cocktail of soluble e2 (s e2) glycoproteins, we detected hcv e2-specific (s e2+) b cells directly ex vivo at multiple acute infection timepoints in 29 hcv-infected subjects with a wide range of anti-e2 ig g titers, including 17 persistently infected subjects and 12 subjects with spontaneous clearance of infection.We performed multi-dimensional flow cytometric analysis of s e2+ and e2-nonspecific (s e2-) class-switched b cells (cs bc).In s e2+ cs bc from both persistence and clearance subjects, frequencies of resting memory b cells (r mbc) were reduced, frequencies of activated mbc (act mbc) and tissue-like mbc (tl mbc) were increased, and expression of fcrl5, an ig g receptor, was significantly upregulated.Across all subjects, plasma anti-e2 ig g levels were positively correlated with frequencies of s e2+ r mbc and s e2+ act mbc, while anti-e2 ig g levels were negatively correlated with levels of fcrl5 expression on s e2+ r mbc and pd-1 expression on s e2+ act mbc.Upregulation of fcrl5 on s e2+ r mbc and upregulation of pd-1 on s e2+ act mbc may limit anti-e2 antibody production in vivo.Strategies that limit upregulation of these molecules could potentially generate higher titers of protective antibodies against hcv or other pathogens.

Journal: PLoS pathogens (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural
Dual-axis control of magnetic anisotropy in a single crystal Co2MnSi thin film through piezo-voltage-induced strain.

Voltage controlled magnetic anisotropy (vcma) has been considered as an effective method in traditional magnetic devices with lower power consumption.In this article, we have investigated the dual-axis control of magnetic anisotropy in co2mn si/ga as/pzt hybrid heterostructures through piezo-voltage-induced strain using longitudinal magneto-optical kerr effect (lmoke) microscopy.The major modification of in-plane magnetic anisotropy of the co2mn si thin film is controlled obviously by the piezo-voltages of the lead zirconate titanate (pzt) piezotransducer, accompanied by the coercivity field and magnetocrystalline anisotropy significantly manipulated.Because in-plane cubic magnetic anisotropy and uniaxial magnetic anisotropy coexist in the co2mn si thin film, the initial double easy axes of cubic split to an easiest axis (square loop) and an easier axis (two-step loop).While the stress direction is parallel to the [1-10] easiest axis (sample i), the square loop of the [1-10] direction could transform to a two-step loop under the negative piezo-voltages (compressed state).At the same time, the initial two-step loop of the [110] axis simultaneously changes to a square loop (the easiest axis).Otherwise, we designed and fabricated the sample ii in which the pzt stress is parallel to the [110] two-step axis.The phenomenon of vcma was also obtained along the [110] and [1-10] directions.However, the manipulated results of sample ii were in contrast to those of the sample i under the piezo-voltages.Thus, an effective dual-axis regulation of the in-plane magnetization rotation was demonstrated in this work.Such a finding proposes a more optimized method for the magnetic logic gates and memories based on voltage-controlled magnetic anisotropy in the future.

Journal: Nanoscale advances (2022)
Publication types: Journal Article
Evaluation of Peri-implant Tissue Stability and Patient Satisfaction After Immediate Implant Placement in the Esthetic Area: A 3-Year Follow-up of an Ongoing Prospective Study.

Horizontal and vertical reduction of the ridge has to be expected after tooth extraction.Immediate implant placement and provisionalization is a viable treatment option that can help to minimize those changes.Additionally, it can better meet a patient's expectations about the treatment, reducing time and invasiveness.The aim of this prospective study is to evaluate the stability of the hard and soft tissues surrounding single immediate implants placed in the esthetic zone, as well as evaluating patient satisfaction.A total of 16 implants were placed, and 15 could be evaluated at the 3-year follow-up.Radiographic and clinical data was recorded after this period.Some marginal bone level reduction was detected after 3 years but was not statistically significant.The soft tissues, measured at 3 points, showed stability and even better positions with respect to the day of the final restoration placement.Patient satisfaction was analyzed using the modified oral health impact profile questionnaire (ohip-14), and high satisfaction values were reported.The implementation of a precise surgical and prosthetic protocol when an immediate implant is placed into a fresh extraction socket is likely to result in high survival and success rates combined with excellent patient satisfaction.After the 3-year follow-up, favorable results were present.

Journal: The International journal of periodontics & restorative dentistry (2020)
Publication types: Journal Article
Significance of Preexisting Vector Immunity and Activation of Innate Responses for Adenoviral Vector-Based Therapy.

An adenoviral (ad v)-based vector system is a promising platform for vaccine development and gene therapy applications.Administration of an ad v vector elicits robust innate immunity, leading to the development of humoral and cellular immune responses against the vector and the transgene antigen, if applicable.The use of high doses (1011-1013virus particles) of an ad v vector, especially for gene therapy applications, could lead to vector toxicity due to excessive levels of innate immune responses, vector interactions with blood factors, or high levels of vector transduction in the liver and spleen.Additionally, the high prevalence of ad v infections in humans or the first inoculation with the ad v vector result in the development of vector-specific immune responses, popularly known as preexisting vector immunity.It significantly reduces the vector efficiency following the use of an ad v vector that is prone to preexisting vector immunity.Several approaches have been developed to overcome this problem.The utilization of rare human ad v types or nonhuman ad vs is the primary strategy to evade preexisting vector immunity.The use of heterologous viral vectors, capsid modification, and vector encapsulation are alternative methods to evade vector immunity.The vectors can be optimized for clinical applications with comprehensive knowledge of ad v vector immunity, toxicity, and circumvention strategies.

Journal: Viruses (2022)
Publication types: Journal Article;Review;Research Support, N.I.H., Extramural;Research Support, U.S. Gov't, Non-P.H.S.
Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts.

This study aimed to further investigate the cytotoxicity against tumor cell lines and several bacterial strains of annona squamosa and its mode of action.Methanol extracts of a.Squamosa leaves (asl) and seeds (ass) were used.Asl showed significant antibacterial activity against s.Aureus, k.Pneumoniae and e.Faecalis with mic values of 78, 78 and 39 μg/m l respectively.Moreover, asl exhibited significant biofilm disruption, rapid time dependent kinetics of bacterial killing, increased membrane permeability and significantly reduced the cell numbers and viability.Regarding the cytotoxicity against tumor cell lines, ass was more active against jurkat and mcf-7 cells, with ci50 1.1 and 2.1 μg/m l, respectively.Asl showed promising activity against jurkat and hl60, with ci50 4.2 and 6.4 μg/m l, respectively.Both extracts showed lower activity against vero cells and reduced the clonogenic survival at higher concentrations (ic90) to mcf-7 and hct-116 lineages.The alkaloids anonaine, asimilobine, corypalmine, liriodenine nornuciferine and reticuline were identified in extracts by uplc-esi-ms/ms analysis.This study reinforced that a.Squamosa presents a remarkable phytomedicinal potential and revealed that its antimicrobial mechanism of action is related to bacterial membrane destabilization.

Journal: Anais da Academia Brasileira de Ciencias (2017)
Publication types: Journal Article
Evaluation of Francisella orientalis ΔpdpA as a Live Attenuated Vaccine against Piscine Francisellosis in Nile Tilapia.

Francisella orientalis is an important bacterial pathogen of marine and freshwater fish with worldwide distribution.Fish francisellosis is a severe subacute to chronic granulomatous disease, with high mortalities and high infectivity rates in cultured and wild fish.To date, there is no approved vaccine for this disease.In this study, we evaluated the efficacy of a defined f.Orientalis pathogenicity determinant protein a (pdp a) mutant (δpdp a) as a live attenuated immersion vaccine against subsequent immersion challenge with the wild-type organism.Immunized nile tilapia oreochromis niloticus were protected (45% relative percent survival) from the lethal challenges and presented significantly lower mortality than nonvaccinated and challenged treatments.Although serum ig m was significantly higher in immunized fish, similar bacterial loads were detected in vaccinated and nonvaccinated survivors.In conclusion, although the f.Orientalis δpdp a is attenuated and effectively stimulated an adaptive immune response, the low relative percent survival and high bacterial persistence in survivors of immunized and challenged treatments indicates low suitability of δpdp a as a mucosal vaccine for tilapia under conditions used in this study.

Journal: Journal of aquatic animal health (2022)
Publication types: Journal Article;Research Support, U.S. Gov't, Non-P.H.S.
Transplant Ureteral Stenosis after Renal Transplantation: Risk Factor Analysis.

Introduction the results of kidney transplants have improved dramatically in recent years, leading to reduced morbidity and mortality.Despite continuous improvements, urological complications occur at a rate of 2.6%-15%.Ureteral stenosis of graft ureters is the most common complication, with a probability of 0.5%-6.3%.This study aimed to determine the incidence of ureteral stenosis after kidney transplantation and identify risk factors that distinguish transplant patients with and without ureteral stenosis.Methods this study retrospectively analyzed patients who had undergone kidney transplantation at the department of urology of the friedrich-alexander university erlangen-nuremberg between 2001 and 2015.Forty-seven patients developed ureteral stenosis during the operation.Most of the ureteral stenosis cases occurred in the first 4 months after transplantation.Kaplan-meier analysis and the log-rank test were used to calculate the cumulative risk, and the mann-whitney u test was used nonparametrically.The significance level was set at p < 0.05.Results statistical analysis showed that residual diuresis (p = 0.008), cold ischemia time (cit) (p = 0.040), the body mass index (p = 0.027), and donor serum creatinine value (p = 0.039) showed a significantly different distribution between recipients with or without ureteral stenosis after kidney transplantation.In multivariate cox's regression modeling, residual diuresis and the donor serum creatinine level were identified as the only independent predictors of patients' stenosis-free survival.Conclusion urological complications not diagnosed and treated in time endanger the success of kidney transplantation.After evaluating the kidney transplantation data of the patients at the transplant center erlangen-nuremberg from 2001 to 2015, residual diuresis, cit, the body mass index, and donor serum creatinine value were found to influence the development of ureteral stenosis.

Journal: Urologia internationalis (2022)
Publication types: Journal Article
Dual role of injectable curcumin-loaded microgels for efficient repair of osteoarthritic cartilage injury.

Curcumin has been widely used for the treatment of age-associated diseases, and showed chondroprotective potential for post-traumatic osteoarthritis (oa).However, due to the irregular-shaped and large-sized defects on joint cartilage in degenerated oa, thein vivodelivery and therapeutic effect of curcumin for effective repair remain challenging.In this study, we first present a peg-gel ma [poly(ethylene glycol) dimethacrylate-gelatin methacrylate, pgms] hydrogel microgel-based curcumin delivery system for both improved anti-inflammatory and pro-regenerative effects in treatment for cartilage defects.The curcumin-loaded pgms were produced by a microfluidic system based on light-induced gelation of gelatin methacrylate (gel ma).This pgms embedding curcumin at a relative low dosage were demonstrated to promote the proliferation and chondrogenic differentiation of mesenchymal stem cellsin vitro.More importantly, the pgms were shown to attenuate the inflammatory response of chondrocytes under il-1β stimulation.Lastly, thein vivoapplication of the injectable pgms significantly promoted the repair of large-sized cartilage injury.These results confirmed that curcumin-loaded pgms can not only enhance the chondroprotective efficacy under inflammatory conditions but also induce efficient cartilage regeneration.This study provides an advanced strategy with anti-inflammatory and pro-regenerative dual-role therapeutic for treatment of extensive cartilage injuries.

Journal: Frontiers in bioengineering and biotechnology (2022)
Publication types: Journal Article
Mapping key neuropeptides involved in the melanocortin system in Atlantic salmon (Salmo salar) brain.

The melanocortin system is a key regulator of appetite and food intake in vertebrates.This system includes the neuropeptides neuropeptide y (npy), agouti-related peptide (agrp), cocaine- and amphetamine-regulated transcript (cart), and pro-opiomelanocortin (pomc).An important center for appetite control in mammals is the hypothalamic arcuate nucleus, with neurons that coexpress either the orexigenic npy/agrp or the anorexigenic cart/pomc neuropeptides.In ray-finned fishes, such a center is less characterized.The atlantic salmon (salmo salar) has multiple genes of these neuropeptides due to whole-genome duplication events.To better understand the potential involvement of the melanocortin system in appetite and food intake control, we have mapped the m rna expression of npy, agrp, cart, and pomc in the brain of atlantic salmon parr using in situ hybridization.After identifying hypothalamic m rna expression, we investigated the possible intracellular coexpression of npy/agrp and cart/pomc in the tuberal hypothalamus by fluorescent in situ hybridization.The results showed that the neuropeptides were widely distributed, especially in sensory and neuroendocrine brain regions.In the hypothalamic lateral tuberal nucleus, the putative homolog to the mammalian arcuate nucleus, npya, agrp1, cart2b, and pomca were predominantly localized in distinct neurons; however, some neurons coexpressed cart2b/pomca.This is the first demonstration of coexpression of cart2b/pomca in the tuberal hypothalamus of a teleost.Collectively, our data suggest that the lateral tuberal nucleus is the center for appetite control in salmon, similar to that of mammals.Extrahypothalamic brain regions might also be involved in regulating food intake, including the olfactory bulb, telencephalon, midbrain, and hindbrain.

Journal: The Journal of comparative neurology (2023)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Dexamethasone for the treatment of established postoperative nausea and vomiting: A randomised dose finding trial.

Dexamethasone is widely used for the prevention of postoperative nausea and vomiting (ponv) but little is known about its efficacy for the treatment of established ponv.Objective to test the antiemetic efficacy of intravenous dexamethasone for the treatment of established ponv in adults undergoing surgery under general anaesthesia and to determine whether there is dose-responsiveness.Design the dex ponv trial is a multicentre, placebo-controlled, randomised, double-blind, dose-finding study.Inclusion of patients was between september 2012 and november 2017.Follow-up for ponv symptoms was for 24 h.Thirty days postoperatively, patients were contacted by study nurses for any information on postoperative bleeding and infection.Setting four public hospitals in switzerland.Patients a total of 803 adults scheduled for elective surgery without any antiemetic prophylaxis signed the consent form; 714 were included.Among those, 319 had ponv and 281 patients were eventually randomised (intention to treat population and safety set).The per protocol set consisted of 260 patients.Interventions patients with ponv symptoms (including retching) were randomised to a single intravenous dose of dexamethasone 3, 6 or 12 mg or matching placebo.Main outcome measures the primary endpoint was the absence of further nausea or vomiting (including retching), within 24 h after administration of the study drug.Results dexamethasone was ineffective during the first 24 h, whatever the dosage, compared to placebo, even when the model was adjusted for known risk factors (p = 0.170).There were no differences in the time to treatment failure or the quality of sleep during the first night.There was a positive correlation between the dose of dexamethasone and blood glucose concentrations (p < 0.001), but not with bleeding risk, wound infections or other adverse effects.Conclusion this randomised trial failed to show anti-emetic efficacy of any of the tested intravenous regimens of dexamethasone for the treatment of established ponv in adults undergoing surgery under general anaesthesia.Trial registration clinicaltrials.gov (nct01975727).

Journal: European journal of anaesthesiology (2022)
Publication types: Journal Article;Multicenter Study;Randomized Controlled Trial
Shared friends counterbalance shared enemies in old forests.

Mycorrhizal mutualisms are nearly ubiquitous across plant communities.Yet, it is still unknown whether facilitation among plants arises primarily from these mycorrhizal networks or from physical and ecological attributes of plants themselves.Here, we tested the relative contributions of mycorrhizae and plants to both positive and negative biotic interactions to determine whether plant-soil feedbacks with mycorrhizae neutralize competition and enemies within multitrophic forest community networks.We used bayesian hierarchical generalized linear modeling to examine mycorrhizal-guild-specific and mortality-cause-specific woody plant survival compiled from a spatially and temporally explicit data set comprising 101,096 woody plants from three mixed-conifer forests across western north america.We found positive plant-soil feedbacks for large-diameter trees: species-rich woody plant communities indirectly promoted large tree survival when connected via mycorrhizal networks.Shared mycorrhizae primarily counterbalanced apparent competition mediated by tree enemies (e.g., bark beetles, soil pathogens) rather than diffuse competition between plants.We did not find the same survival benefits for small trees or shrubs.Our findings suggest that lower large-diameter tree mortality susceptibility in species-rich temperate forests resulted from greater access to shared mycorrhizal networks.The interrelated importance of aboveground and belowground biodiversity to large tree survival may be critical for counteracting increasing pathogen, bark beetle, and density threats.

Journal: Ecology (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't;Research Support, U.S. Gov't, Non-P.H.S.
Glycemic Index of Slowly Digestible Carbohydrate Alone and in Powdered Drink-Mix.

Consumer interest in food and beverages with carbohydrates offering steady glucose release and lower glycemic index (gi) continues to rise.Glycemic index is one of the metrics for carbohydrate quality.Slowly digestible carbohydrates (sdc) offer an ingredient solution to improve carbohydrate quality and meet consumer needs.Sustratm2434 slowly digestible carbohydrate is a blend of tapioca flour and corn starch.The study objective was to determine the glycemic index of the sdc ingredient alone and in a powdered drink-mix.In a randomized, single-blind study, heathy adults (n = 14) consumed four test drinks, delivering 50 g available carbohydrates on separate days to measure gi.Participants either consumed dextrose in water (placebo), sdc ingredient in water, sdc drink-mix powder reconstituted in skim milk, or control drink-mix reconstituted in skim milk (without sdc).Post-prandial glucose response was measured over 4 h.Sdc exhibited lower gi (0-2 h) and higher steady glucose release (beyond 2 h).Sdc alone (gi = 27) and sdc in drink-mix (gi = 30.3) showed significantly lower gi (-27%) compared to dextrose (100) and the control drink-mix (41.5).Sustratm2434 sdc is a low glycemic ingredient, suitable for product innovations with potential for low glycemic and steady glucose release claims.

Journal: Nutrients (2019)
Publication types: Comparative Study;Journal Article;Randomized Controlled Trial