syedzayyan/paper_mill_benchmark huggingface dataset:
The Molecular Mechanism of Hepatic Lipid Metabolism Disorder Caused by NaAsO2 through Regulating the ERK/PPAR Signaling Pathway

Chronic arsenic exposure is a risk factor for human fatty liver disease, and the erk signaling pathway plays an important role in the regulation of liver lipid metabolism.However, whether erk plays a role in the progression of arsenic-induced liver lipid metabolism disorder and the specific mechanism remain unclear.Here, by constructing a rat model of liver lipid metabolism disorder induced by chronic arsenic exposure, we demonstrated that erk might regulate arsenic-induced liver lipid metabolism disorders through the ppar signaling pathway.Arsenic could upregulate the expression of pparγ and cd36 in the rat liver, decrease the expression of pparα and cpt-1 in the rat liver, increase the organ coefficient of the rat liver, decrease the content of tg in rat serum, and promote fat deposition in the rat liver.In the arsenic-induced rat model of hepatic lipid metabolism disorder, we found that the expression of p-erk was increased.In order to further explore whether the erk signaling pathway was involved in arsenic-induced liver lipid metabolism disorder, we exposed l-02 cells to different arsenic concentrations, and the results showed that arsenic significantly increased the expression of p-erk in l-02 cells in a dose-dependent manner.We further treated l-02 cells with erk inhibitors and found that the expression of tg, pparα, and cpt-1 in l-02 cells increased, while the expression of p-erk, pparγ, and cd36 decreased.In conclusion, erk may be involved in arsenic-induced liver lipid metabolism disorder by regulating the ppar signaling pathway.These findings are expected to provide a new targeting strategy for arsenic-induced liver lipid metabolism disorder.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
The Impact of Catalpol on Proliferation, Apoptosis, Migration, and Oxidative Stress of Lung Cancer Cells Based on Nrf2/ARE Signaling

The effects of catalpol on lung cancer cell proliferation, apoptosis, migration, and oxidative stress via the nrf2/are signaling pathway are investigated in this work.Catalpol‐12 g/m l group, catalpol‐24 g/m l group, catalpol‐48 g/m l group, catalpol − 48 g/m l + vector group, catalpol − 48 g/m l + nrf2 group, si‐nc group, and si‐nrf2 group were used to split lung cancer cells a549 into control groups.Proliferation was detected using the cck‐8 assay; apoptosis was detected using flow cytometry; migration was detected using the transwell chamber; ros was distinguished using the dcfhda method; mda, sod, and gsh were detected using the microvolume method; and cleaved caspase‐3, cleaved caspase‐9, nrf2, ho‐1, mmp‐9, and mmp‐2 were detected using the western blot method.Catalpol 12 g/m l and 24 g/m l‐48 g/m l treatment decreased the proliferation activity, migration number, and nrf2, ho‐1, mmp‐9, and mmp‐2 protein levels of lung cancer cells when compared to the control group.Sod and gsh levels of lung cancer cells were decreased, and mda and ros levels were increased.Cleaved caspase‐3, cleaved caspase‐9 protein expression levels, and apoptosis were boosted ( p &lt; 0.05).The proliferation activity, migration number, and protein levels of nrf2, ho‐1, mmp‐9, and mmp‐2 in the catalpol − 48 g/m l + nrf2 group were raised compared to the catalpol − 48 g/m l + vector group, whereas there was an apparent drop in the cleaved caspase‐3, cleaved caspase‐9, and apoptosis rate.Similarly, sod and gsh contents increased, whereas mda and ros decreased ( p &lt; 0.05).The proliferation activity, migration number, and nrf2, ho‐1, mmp‐9, and mmp‐2 protein levels of lung cancer cells in the si‐nrf2 group were all decreased when compared to the si‐nc and control groups.Cleaved caspase‐3 and cleaved caspase‐9 protein expression, on the other hand, increased as mda and ros levels were raised while sod and gsh levels dropped ( p &lt; 0.05).It reveals that catalpol inhibits the nrf2/are signaling pathway, which causes antiproliferation, migration, apoptosis, and oxidative stress in cancer cells of lungs.The rate of apoptosis was also lowered.

Journal: BioMed Research International (2022)
Publication types: Research Article;
The Efficacy and Safety of Oral Nutrient Solution Supplementation in Patients with Esophageal Cancer under Nutritional, Psychological, Physical, and Prognostic Survival Differences

Inflammasomes are massive intracellular multiprotein signaling complexes produced in the cytosolic compartment.Tumor etiology involves dysregulation of nlrp3 inflammasome activation.Current studies are being conducted to ascertain the effectiveness and safety of oral nutrient solution supplementation in patients with esophageal cancer based on the current understanding of the role of the nlrp3 inflammasome in possible cancer promotion and therapy.This study examines how oral nutrient solution supplement therapy affects patients receiving radiotherapy and chemotherapy for esophageal cancer in terms of nutrition, physical composition, quality of life, psychology, prognosis, and survival.A total of 120 patients with esophageal cancer admitted to our hospital from march 2020 to january 2021 were selected for the current study.The selected 120 patients are divided into nutritional supplement and traditional groups according to the random number table method, with 60 cases in each group.The nutritional supplement group received an oral nutritional solution, whereas the conventional group received the ordinary dietary intervention.Since radiotherapy can potentially damage healthy tissue with short‐ and long‐term negative effects.Inflammatory reactions are a common component of adverse effects of early and late ionizing radiation.Oral nutrient solution supplement therapy for patients with esophageal cancer after radiotherapy and chemotherapy can significantly improve the levels of blood nutrients, later physical components, and quality of life of patients.

Journal: BioMed Research International (2023)
Publication types: Clinical Study;
The Effects of Statin Therapy on Oxidized LDL and Its Antibodies: A Systematic Review and Meta-Analysis

Elevated serum low-density lipoproteins (ldl), the substrate for the formation of atherogenic oxidized ldls (ox ldl), are a causal factor for atherosclerotic cardiovascular disease (ascvd).Statins are well known to decrease ldl particle concentration and reduce ascvd morbidity and mortality.Objective.To perform a meta-analysis of the effects of statins (i.e., type, dose, and duration of treatment) on serum levels of ox ldl and on immunoglobulin m (ig m) and immunoglobulin g (ig g) antibody levels against ox ldl.Methods.Pub med, scopus, embase, and web of science were searched up to february 5th, 2021, for randomized controlled trials (rct) evaluating the effect of statins on ox ldl and anti-ox ldl antibody levels.Meta-analysis was performed using comprehensive meta-analysis (cma) v2 software.To evaluate the influence of each study on the overall effect size, a sensitivity analysis was performed using the leave-one-out method.Evaluation of the funnel plot, begg’s rank correlation, and egger’s weighted regression tests was used to assess the presence of publication bias in the meta-analysis.Results.A total of 28 rcts including 4019 subjects were finally included in the meta-analysis.The results indicated a significant decrease in circulating concentrations of ox ldl after treatment with statins (smd: -2.150, 95% ci: -2.640, -1.697, p &lt; 0.001 ).Subgroup analysis found no significant effect of the intensity of statin treatment or statin lipophilicity on the reduction of circulating concentrations of ox ldl.An additional meta-analysis of 3 trials showed that statins did not change the serum levels of ig m and ig g antibodies to ox ldl.Conclusion.Statin therapy decreases serum ox ldl concentrations but does not affect circulating levels of anti-ox ldl antibodies.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Meta-Analysis;
The Effect of Calisthenics on Hypoglycemic of Diabetic Patients

To analyze the effect of calisthenics on hypoglycemic in diabetic patients.Method .From september 2019 to may 2020, 210 patients with type 2 diabetes who were newly diagnosed in our hospital were chosen.They were split into two groups: observation ( n = 105) and control ( n = 105).Only drug therapy and diet management were given to the control group, whereas the observation group was given calisthenics treatment in addition to regular diet control and medicine.The outcomes of the two groups of patient’s blood glucose levels, bmi, quality of life, and blood lipid index were compared in this study.Results .Before treatment, there was no significant difference in fpg, 2h pg, and hb a1c levels between the two groups ( p &gt; 0.05).Compared with before treatment, fpg, 2h pg, and hb a1c were significantly reduced in both groups ( p &lt; 0.05).In addition, fpg, 2h pg, and hb a1c in the observation group were significantly lower than those in the control group ( p &lt; 0.05).There was no significant difference in bmi between the two groups before treatment ( p &gt; 0.05).After treatment, the bmi of the two groups was significantly reduced ( p &lt; 0.05), and the bmi of the observation group was significantly lower than that of the control group ( p &lt; 0.05).A comparison of the two groups’ sf‐36 scale scores before intervention revealed no statistically significant difference ( p &gt; 0.05).The observation group’s sf‐36 scores were substantially higher than the control group’s after intervention ( p &lt; 0.05).After treatment, the tc and hdl‐c levels in the observation group were not significantly different from those in the control group ( p &gt; 0.05).Compared with the control group, tg and ldl‐c levels were significantly decreased, with statistically significant differences ( p &lt; 0.05).Conclusion .Based on routine nursing intervention, using calisthenics to treat blood sugar has a significant effect, it not only made the patient’s self‐care ability significantly improved, which was worthy of active promotion in clinical practice.

Journal: BioMed Research International (2022)
Publication types: Research Article;
The Disparity in the Management of Polycystic Ovary Syndrome between Obstetrician-Gynecologists in Different-Level Hospitals under the Hierarchical Medical System

Pcos is a prevalent endocrine and metabolic disorder in women characterized by abnormal blood glucose, dyslipidemia, and abnormal mental health.To improve patient care, the goal of our study is to find out if there are differences in how pcos patients are treated at different hospital levels within the hierarchical medical system.Methods .Obstetricians and gynecologists from primary, secondary, and tertiary hospitals were the participants in the survey.The responses provided and collected were analyzed using various statistical techniques like the chi‐square test, fisher exact test, and logistic regression with multiple variables.Results .The investigation examined 2298 survey replies (13.1% primary hospitals, 52.4% secondary hospitals, and 34.5% tertiary hospitals).As hospital grade increases, more participants inquire about a patient’s history of unfavorable pregnancies concerning hormone evaluation; the better the hospital’s grade, the greater the number of participants who would undergo amh and androgen‐related tests.The higher the hospital level, the more participants would pick the oral glucose tolerance test (ogtt) to determine insulin resistance, the bmi asian criteria for defining obesity, and blood lipids.Participants in primary (odds ratio (or) = 0.383, 95% confidence interval (ci) 0.282‐0.520) and secondary (or = 0.607, 95% confidence interval (ci) 0.481‐0.765) hospitals were significantly less likely to select ogtt than those in tertiary hospitals.Comparatively, fewer primary hospitals chose to do lipid profiling than tertiary hospitals (or 0.689, 95% ci 0.523‐0.909).With the increase in hospital level, participants were more knowledgeable about the multiple efficacies and dose alternatives of metformin and selected letrozole and assisted reproduction more frequently.Conclusion .Our study uncovered differences in the endocrine evaluation, metabolic screening, and management of pcos patients across obstetrics and gynecology at various hospital levels.Simultaneously, it underlines the need to improve the hierarchical medical system and close the knowledge gap across hospitals.

Journal: BioMed Research International (2022)
Publication types: Research Article;
The Correlation of PM2.5 Exposure with Acute Attack and Steroid Sensitivity in Asthma

Bronchial asthma is a common chronic inflammatory disease of the respiratory system.Asthma primarily manifests in reversible airflow limitation and airway inflammation, airway remodeling, and persistent airway hyperresponsiveness.Pm2.5, also known as fine particulate matter, is the main component of air pollution and refers to particulate matter with an aerodynamic diameter of ≤2.5 μ m.Pm2.5 can be suspended in the air for an extensive time and, in addition, can contain or adsorb heavy metals, toxic gases, polycyclic aromatic hydrocarbons, bacterial viruses, and other harmful substances.Epidemiological studies have demonstrated that, in addition to increasing the incidence of asthma, pm2.5 exposure results in a significant increase in the incidence of hospital visits and deaths due to acute asthma attacks.Furthermore, pm2.5 was reported to induce glucocorticoid resistance in asthmatic individuals.Although various countries have implemented strict control measures, due to the wide range of pm2.5 sources, complex components, and unknown pathogenic mechanisms involving the atmosphere, environment, chemistry, and toxicology, pm2.5 damage to human health still cannot be effectively controlled.In this present review, we summarized the current knowledge base regarding the relationship between pm2.5 toxicity and the onset, acute attack prevalence, and steroid sensitivity in asthma.

Journal: BioMed Research International (2022)
Publication types: Review Article;
The Connection between High Myopia Patients and MiR-708a or MiR-148 Expression Levels in Aqueous Studies of Visual Acuity

Myopia goes far beyond the inconvenience it brings.It is a prevailing and vision‐threatening eye disease, especially in asia.Aberrantly expressed mi r‐708a and mi r‐148 are critical for accurate diagnosis, good prognosis, and precise response prediction of myopia.In this paper, we aim to examine the potential contributions of mi r‐708a, mi r‐148a, and pax6 to high myopia (hm).First, aqueous samples were taken from 25 exclusively hm eyes and 25 exclusively cataract eyes.For next‐generation sequencing and bioinformatics analysis, rna from sample 30one was used.Twenty more samples were used for rt‐q pcr.341 mi rnas in total were found in hm eyes; 249 mature mi rnas and 17 new mi rnas showed differential expression.The expression of hsa‐mi r‐127‐3p, hsa‐let‐7i‐5p, and hsa‐mi r‐98‐5p was identified using rt‐q pcr.Mi r‐708a and mi r‐148, which may be linked to the development of myopia and serve as possible biomarkers, are notably highly expressed in atrial tissues of hm patients.Our findings may help deepen the understanding of the mechanisms behind the high expression of mi r‐708a and mi r‐148 in atrial tissues of patients with hm.

Journal: BioMed Research International (2022)
Publication types: Research Article;
The Comparative Pharmaco- and Histokinetics of the Therapeutic Dose of Estradiol Valerate and Bromocriptine in Common Quails

The current study is aimed at examining the overall effects of steroids on the tissues of organisms and pharmacotherapeutics and pharmaco‐histokinetics of several steroids, including bromocriptine as mesylate and estradiol valerate in common quails ( coturnix coturnix ).A total of 100 birds were used for pharmaco‐histokinetics.The research was carried out in two separate trials, one during the fall season and the other during the spring season.Each experiment lasted for five, ten, fifteen, and twenty days.Each study group used 20 birds while basing their experiments on a control group of 5.At the stretch of five, ten, fifteen, and twenty days in each season, therapeutic dosages were administered to a sum of two groups representing two separate steroid trial groups.Each steroid was administered to each bird in a therapeutic dose, which was three drops administered twice daily.Clinical symptoms include despondency, sluggishness, and variations in weight and temperature that almost all treated birds display.However, only in trials conducted in the fall was a sizable degree of body enlargement in one treated bird noticed.The winter testing showed a mortality rate.Four birds have died in the twenty‐day group.One bird died when treated with estradiol valerate, and three birds died treated with bromocriptine as mesylate.Both the male and female birds showed signs of having lost some of their body weight.The treated birds’ kidney, stomach, hearts, and livers exhibited some edema.In comparison, almost all birds show enteritis, which indicates that steroids mainly affect the intestine.There were apparent differences in the histological analysis of heart and skeletal muscle and some treated birds with the control group.The kidney, liver, and intestine show the major histopathological change in all treated birds.

Journal: BioMed Research International (2022)
Publication types: Research Article;
MCCK1 enhances the anticancer effect of temozolomide in attenuating the invasion, migration and epithelial-mesenchymal transition of glioblastoma cells in vitro and in vivo

Chemotherapy with temozolomide (tmz) is the traditional treatment for glioblastoma (gbm).Nevertheless, majority of gbm patients have recurrence from resistance to the chemotherapy.Herein, we examined combinational effects of mcck1 (a specific and effective ikkε inhibitor) with tmz in gbm u251mg and u‐87mg cell lines as well as u251mg xenograft models to overcome the therapeutic limitation of chemotherapy for gbm.Although mcck1 alone showed inhibitory effects on in vitro proliferation, migration, invasion, and emt of u251mg and u‐87mg cells, combination of mcck1 and tmz showed enhanced inhibitory effects.In the u251mg gbm xenograft models, mcck1 showed synergistic therapeutic effects in combination with tmz to reduce tumor volumes significantly.These data indicated that mcck1 could be a candidate sensitizer to potentiate therapeutic effects of conventional cytotoxic treatment for gbm.

Journal: Cancer Medicine (2019)
Publication types: Research Article;
Puerarin 6″-O-xyloside suppressed HCC via regulating proliferation, stemness, and apoptosis with inhibited PI3K/AKT/mTOR

Puerarin 6′′‐o‐xyloside is a tumor suppressive derivate of puerarin that is recently characterized as a lysine‐specific demethylase 6b inhibitor.Here we investigated the effects of puerarin 6′′‐o‐xyloside in hepatocellular carcinoma (hcc) cell lines smmc‐7721 and hep g2.Cell viability, proliferation, stemness, protein expression, and autophagy were tested by cck‐8, colony formation, sphere formation, western blotting, and lc3b gfp puncta per cell, respectively.Apoptosis, cd133‐positive cells, and jc‐1‐labeled mitochondrial membrane potential were measured by flow cytometry.The effects of puerarin 6′′‐o‐xyloside in vivo were explored in hep g2 xenograft mice.Puerarin 6′′‐o‐xyloside inhibited cell viability, proliferation, and stemness, and promoted apoptosis in both smmc‐7721 and hep g2 cells.Further experiments showed promoted autophagy and decreased mitochondrial membrane potential, and decreased expression of p‐pi3k, p‐akt, and p‐m tor in hep g2 cells.Co‐administration of 3‐ma with puerarin 6′′‐o‐xyloside obviously augmented these effects including inhibited protein expression of p‐pi3k, p‐akt, and p‐m tor, and inhibited proliferation, promoted apoptosis, and decreased stemness.In hep g2 xenograft mice, 100 mg/kg/d puerarin 6′′‐o‐xyloside significantly suppressed tumor growth, stemness, and apoptosis.In conclusion, our study indicated that puerarin 6′′‐o‐xyloside decreased cell viability, proliferation, and stemness, and promoted autophagy and mitochondria‐dependent apoptosis of hcc, at least partly through inhibiting pi3k/akt/m tor.These results highlighted puerarin 6′′‐o‐xyloside as a promising prodrug that could inhibit both pi3k/akt/m tor and epigenetic demethylation.

Journal: Cancer Medicine (2020)
Publication types: Research Article;
Long noncoding RNA CASC9 promotes the proliferation and metastasis of papillary thyroid cancer via sponging miR-488-3p

Cancer susceptibility candidate 9 (casc9) is a recently identified lnc rna that acted as a tumor promotor in diversified cancer types.However, its role in papillary thyroid cancer (ptc) remains unknown.The expression of casc9 was measured in 52 human ptc tissues and ptc cell lines as well as their controls.The proliferation, migration, and invasion of ptc cells were determined after knockdown or overexpression of casc9 to evaluate the effect of casc9 on ptc cells.Also, the role of ptc tumorigenesis was confirmed in mice xenograft models.Additionally, the underlying mechanisms of casc9 were further researched.We found that casc9 expression was augmented in human ptc tissues and cells.Higher casc9 expression was associated with large tumor size, advanced stage, or lymph node metastasis.Downregulation of casc9 significantly attenuated the proliferative, migrative, and invasive abilities of ptc cells, and suppressed tumorigenesis in vivo.While overexpression of casc9 elevated the proliferation, migration, and invasion of ptc cells.Mi r‐488‐3p expression was decreased, and adam9 level was increased in ptc tissues and cells.Casc9 expression was negatively related to mi r‐488‐3p, but positively associated with adam9 expression in ptc tissues.Molecular mechanism analysis revealed that casc9 functioned via sponging mi r‐488‐3p to regulate adam9 expression, followed by activation of egfr‐akt signaling.In conclusion, lnc rna casc9 promoted the malignant phenotypes of ptc via modulating mi r‐488‐3p/adam9 pathway.This study may provide a novel therapeutic target for the treatment of ptc.

Journal: Cancer Medicine (2020)
Publication types: Research Article;
The Association between the Risk of Esophageal Cancer and Type 2 Diabetes Mellitus: An Updated Meta-Analysis

A large amount of publications had reported the association between incidence of esophageal cancer (ec) and type 2 diabetes mellitus (t2dm) in the past decade.However, those papers’ results are inconsistent on relationships between t2dm the incidence of ec.Therefore, the objective of this meta‐analysis was to determine the relationship between t2dm and the risk of ec (including 2 histological types, esophageal adenocarcinoma [eadc] and esophageal squamous cell carcinoma [escc]).Method .We finally extracted 19 articles though pubmed, embased, and cochrane library.Those identify extraction date including 14,312 cases and 24,959,067 control records and then mixed the relative risks (rrs) and corresponding 95% confidence intervals (95%cis) through stata.Results .We observed that there are significantly positive correlation between t2dm and ec risk ( r r = 1.28, 95% ci: 1.05‐1.57, p = 0.015).also, our study showed positive correlation between t2dm and eadc (esophageal adenocarcinoma) risk ( r r = 1.28, 95% ci: 1.05‐1.57, p &lt; 0.001).What’s more, subgroup analysis based on ethnicity represented the caucasian is more susceptible to ec ( r r = 1.28 ,95% ci: 1.10‐1.49, p = 0.001).Conclusion .Those results offer a recent epidemiological and integrated evidence to ascertain the correlations between t2dm and incidence of ec.Those results take public health implications on preventing t2dm and then depress the occurrence of ec.Our study also provides referenced information for the prevention.However, some data is still insufficient, and more research should be carried out.

Journal: BioMed Research International (2022)
Publication types: Meta-Analysis;