syedzayyan/paper_mill_benchmark huggingface dataset:
Impact of photobiomodulation on external root resorption during orthodontic tooth movement in humans - A systematic review and meta-analysis.

Photobiomodulation has been gaining traction as a plausible therapy to control orthodontically induced root resorption.Aim the aim of the present review was to systematically appraise randomized controlled trials conducted to study the influence of photobiomodulation on external root resorption during orthodontic movement in humans.Method ology - a systematic search was carried out employing keywords in various electronic databases namely medline (pubmed), cochrane library, google scholar, semantic scholar, science direct and opengrey.eu for studies up to march 2020.Pre-defined inclusion and exclusion criteria were used to select the studies.Data extraction was carried out and the risk of bias was assessed using cochrane risk of bias tool.Meta-analysis was conducted using random effects model for selected studies.Subgroup analysis was conducted for resorption on each axial surface of the tooth root viz.Mesial, buccal, distal and palatal as well as for vertical thirds viz.Cervical, middle and apical third.Summary of findings was formulated according to grade profile.Results the search retrieved 1509 results out of which six studies were included for the systematic review.Two studies showed low overall risk of bias and the remaining four showed unclear risk of bias.The meta-analysis was conducted for three studies with an overall sample size of 120 teeth which showed a pooled mean difference of 0.08 (95% ci 0.15 - (-0.02) to 1.96, p=<0.0001) in favour of photobiomodulation group with respect to mean total resorption per tooth.I2index revealed 88% heterogeneity.Conclusion it is concluded that there is moderate grade of evidence to suggest beneficial effect of photobiomodulation on root resorption.Further high-quality randomized controlled trials with standardized intervention parameters are recommended.Registration prospero registration number - crd42020167291.

Journal: Journal of oral biology and craniofacial research (2022)
Publication types: Journal Article;Review
Insights into the rapid elimination of antibiotics from aqueous media by tunable C3N4photocatalysts: Effects of dopant amount, co-existing ions and reactive oxygen species.

Conventional photocatalytic nanomaterials are not environmentally sustainable since these are usually produced from scarce mineral and metal precursors.Moreover, high pollutant removal efficiencies by conventional photocatalysts are only attained after several hours of reaction with light.In this study, novel visible light-active photocatalysts were synthesized from environment-friendly carbon precursors and applied for the rapid degradation of sulfamethoxazole (smx) in aqueous solutions.The photocatalysts were prepared via the co-pyrolysis of urea with varying doping temperature and dopant amount.These variations played a vital role in improving the performance of the photocatalysts and resulted in up to >99% smx removal within 45 min of visible light irradiation.Characterization of the photocatalysts showed that potassium and iodine dopants were responsible in the red shift and broadening of the light absorption spectrum to the visible region.In addition, the band gap energy narrowed by 0.23 e v resulting in faster charge transfer but slower recombination of the photo-generated electron and hole pairs.Effects of varying concentrations of inorganic salts (no3-, so42-, cl-, po43-) on smx removal were also examined.Lastly, the mechanism of smx photodegradation was elucidated.

Journal: The Science of the total environment (2019)
Publication types: Journal Article
Comparative Study on Grape Berry Anthocyanins of Various Teinturier Varieties.

The red-fleshed grape cultivars, called teinturier or dyer grapes, contain anthocyanins in both the skin and flesh.These phenolic compounds exhibit excellent coloring ability, and as antioxidants, they are important bioactive compounds in food crops.In this work, anthocyanin patterns of grape berries of fifteen teinturier varieties collected from the gene bank located at pécs in the southwest of hungary were compared.Anthocyanin profiles of numerous varieties originating from hungary such as 'bíborkadarka', 'kármin', 'kurucvér', and 'turán' are reported for the first time.Anthocyanins extracted separately from the skin and juice were analyzed using high-performance liquid chromatography coupled with a photodiode array detector.For the identification of compounds, high-resolution orbitrap mass spectrometry was used.All in all, twenty-one anthocyanins were identified and quantified.We found that anthocyanin patterns differed significantly in the skin and juice for all investigated cultivars.For vitis viniferavarieties, the predominant anthocyanin in the skin was malvidin-3-o-glucoside, while the main pigment in the juice was peonidin-3-o-glucoside.For the first time, a significant amount of diglucosides was detected in two vitis viniferacultivars with a direct relationship.In general, the pigment composition of the skin was much more complex than that of the juice.The comparative study with presented patterns gives valuable and beneficial information from a chemotaxonomical point of view.Our results also help to choose the appropriate teinturier varieties with the desired anthocyanins for food coloring or winemaking purposes.

Journal: Foods (Basel, Switzerland) (2022)
Publication types: Journal Article
Tunicamycin as a Novel Redifferentiation Agent in Radioiodine Therapy for Anaplastic Thyroid Cancer.

The silencing of thyroid-related genes presents difficulties in radioiodine therapy for anaplastic thyroid cancers (atcs).Tunicamycin (tm), an n-linked glycosylation inhibitor, is an anticancer drug.Herein, we investigated tm-induced restoration of responsiveness to radioiodine therapy in radioiodine refractory atcs.125i uptake increased in tm-treated atc cell lines, including bht101 and cal62, which was inhibited by kcl o4, a sodium-iodide symporter (nis) inhibitor.Tm upregulated the m rna expression of iodide-handling genes and the protein expression of nis.Tm blocked p erk1/2 phosphorylation in both cell lines, but akt (protein kinase b) phosphorylation was only observed in cal62 cells.The downregulation of glucose transporter 1 protein was confirmed in tm-treated cells, with a significant reduction in18f-fluorodeoxyglucose (fdg) uptake.A significant reduction in colony-forming ability and marked tumor growth inhibition were observed in the combination group.Tm was revealed to possess a novel function as a redifferentiation inducer in atc as it induces the restoration of iodide-handling gene expression and radioiodine avidity, thereby facilitating effective radioiodine therapy.

Journal: International journal of molecular sciences (2021)
Publication types: Journal Article
Safety and efficacy of a novel robotic transcranial doppler system in subarachnoid hemorrhage.

Delayed cerebral ischemia (dci) secondary to vasospasm is a determinate of outcomes following non-traumatic subarachnoid hemorrhage (sah).Sah patients are monitored using transcranial doppler (tcd) to measure cerebral blood flow velocities (cbfv).However, the accuracy and precision of manually acquired tcd can be operator dependent.The nova guide robotic tcd system attempts to standardize acquisition.This investigation evaluated the safety and efficacy of the nova guide system in sah patients in a neuro icu.We retrospectively identified 48 nova guide scans conducted on sah patients.Mean and maximum middle cerebral artery (mca) cbfv were obtained from the nova guide and the level of agreement between cbfv and computed tomography angiography (cta) for vasospasm was determined.Safety of nova guide acquisition of cbfv was evaluated based on number of complications with central venous lines (cvl) and external ventricular drains (evd).There was significant agreement between the nova guide and cta (cohen's kappa = 0.74) when maximum mca cbfv ≥ 120 cm/s was the threshold for vasospasm.27/48 scans were carried out with cvls and evds present without negative outcomes.The lack of adverse events associated with evds/cvls and the strong congruence between maximal mca cbfv and cta illustrates the diagnostic utility of the nova guide.

Journal: Scientific reports (2022)
Publication types: Journal Article
Engineering 3D Cortical Spheroids for anIn VitroIschemic Stroke Model.

Three-dimensional (3d) spheroids composed of brain cells have shown great potential to mimic the pathophysiology of the brain.However, a 3d spheroidal brain-disease model for cerebral ischemia has not been reported.This study investigated an ultralow attachment (ula) surface-mediated formation of 3d cortical spheroids using primary rat cortical cells to recapitulate the cerebral ischemic responses in stroke by oxygen-glucose deprivation-reoxygenation (ogd-r) treatment.Comparison between two-dimensional (2d) and 3d cell culture models confirmed the better performance of the 3d cortical spheroids as normal brain models.The cortical cells cultured in 3d maintained their healthy physiological morphology of a less activated state and suppressed m rna expressions of pathological stroke markers, s100b, il-1β, and mbp, selected based onin vivostroke model.Interestingly, the spheroids formed on the ula surface exhibited striking aggregation dynamics involving active cell-substrate interactions, whereas those formed on the agarose surface aggregated passively by the convective flow of the media.Accordingly, ula spheroids manifested a layered arrangement of neurons and astrocytes with higher expressions of integrin β1, integrin α5, n-cadherin, and fibronectin than the agarose spheroids.Ogd-r-induced stroke model of the ula spheroids successfully mimicked the ischemic response as evidenced by the upregulated m rna expressions of the key markers for stroke, s100b, il-1β, and mbp.Our study suggested that structurally and functionally distinct cortical spheroids could be generated by simply tuning the cell-substrate binding activities during dynamic spheroidal formation, which should be an essential factor to consider in establishing a brain-disease model.

Journal: ACS biomaterials science & engineering (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Adult spinal opioid receptor μ1 expression after incision is altered by early life repetitive tactile and noxious procedures in rats.

Clinical and experimental data suggests that noxious stimulation at critical stages of development results in long-term changes on nociceptive processing in later life.Here, we use an established, well-documented rat model of repetitive noxious procedures closely mimicking the clinical situation in the nicu.In order to understand molecular changes underlying the long-term consequences of repetitive stimulation of the developing nociceptive system the present study aims to analyze the presence of the μ-opioid-receptor-1 (oprm1).Neonatal rats received either four needle pricks per day in the left hind-paw from postnatal day 0-7 as a model of procedural pain in infancy.Control pups were handled in the same way but were instead tactile stimulated, or were left undisturbed.At the age of 8 weeks, all animals received an ipsilateral hind-paw incision as a model for post-operative pain, and mechanical sensitivity was tested at multiple time-points.Before, and 1 or 5 days post-incision, spinal cord tissue was collected for immunostaining of opioid receptor oprm1.Semi-quantitative immunocytochemical analysis of superficial laminae in lumbar spinal dorsal horn revealed that: (1) early life repetitive tactile or noxious procedures do not alter baseline levels of oprm1 staining intensity and (2) early life repetitive tactile or noxious procedures lead to a decrease in oprm1 staining intensity 5 days after incision in adulthood compared to undisturbed controls.We conclude that early life repetitive tactile or noxious procedures affect the intensity of oprm1-immunoreactivity in the lumbar superficial spinal cord dorsal horn after adulthood injury, without affecting baseline intensity.© 2018 the authors.Developmental neurobiology published by wiley periodicals, inc.Develop neurobiol 78: 417-426, 2018.

Journal: Developmental neurobiology (2018)
Publication types: Journal Article
The vascular surgery in-training examination predicts performance on qualifying examination.

Vascular surgery trainees participate in the vascular surgery in-training examination (vsite) during each year of their training.Although the vsite was developed as a low-stakes, formative examination, performance on that examination might correlate with the pass rates for the vascular surgery board written qualifying examination (vqe) and oral certifying examination (vce) and might, therefore, guide both trainees and program directors.The present study was designed to examine the ability of the vsite to predict trainees' performance on the vqe and vce.Methods all first-time candidates of the vascular surgery board vqe and vce were analyzed from 2016 to 2020, including those from both the integrated and independent training pathways.Vsite scores from the final year of training were associated with the vqe scores and the probability of passing the vqe and vce both.Linear and logistic regression models were used to determine the ability of vsite results to predict the vqe scores and the probability of passing each board examination.Results vsite scores available for the 559 candidates (69.3% male; 30.7% female) who had completed the vqe and 369 candidates (66.7% male; 33.3% female) who had completed both the vqe and the vce.The linear regression model results for the final year of training showed that the vsite scores explained 34% of the variance in the vqe scores (29% for the integrated and 37% for the independent trainees).Logistic regression demonstrated that the final year vsite scores were a significant predictor of passing the vqe for both integrated and independent trainees (p 90% for each group of candidates.The probability of passing the vqe decreased to 73% for candidates from integrated programs, 61% for candidates from independent programs, and 64% for the whole cohort when the score was 400.The vsite scores were a significant predictor of passing the vce only for the candidates from independent programs (odds ratio, 1.01; 95% confidence interval, 1.00-1.02; p < .01), for whom a vsite score of 400 correlated with an 82% probability of passing the vce.Conclusions vsite performance is predictive of passing the vqe for trainees from both integrated and independent training paradigms.Vascular surgery trainees and training programs should optimize their preparation and educational efforts to maximize performance on the vsite during their final year of training to improve the likelihood of passing the vqe.Further analysis of the predictive value of vsite scores during the earlier years of training might allow the board certification examinations to be administered earlier in the final year of training.

Journal: Journal of vascular surgery (2022)
Publication types: Journal Article
Heat-induced transitions of an empty minute virus of mice capsid in explicit water: all-atom MD simulation.

The capsid-like structure of the virus-based protein nanoparticles (nps) can serve as bionanomaterials, with applications in biomedicines and nanotechnology.Release of packaged material from these nanocontainers is associated with subtle conformational changes of the np structure, whichin vitro, is readily accomplished by heating.Characterizing the structural changes as a function of temperature may provide fresh insights into nanomaterial/antiviral strategies.Here, we have calculated heat induced changes in the properties of an empty minute virus of mice particle using large-scale ≈ 3.0 × 106all-atom molecular dynamics simulations.We focus on two heat induced structural changes of the np, namely, dynamical transition (dt) and breathing transition (bt), both characterized by sudden and sharp change of measured parameters at temperatures, tdtand tbt, respectively.While dt is assessed by mean-square fluctuation of hydrogen atoms of the np, bt is monitored through internal volume and permeation rate of water molecules through the np.Both the transitions, resulting primarily from collective atomistic motion, are found to occur at temperatures widely separated from one another (tbt>tdt).The breathing motions, responsible for the translocation events of the packaged materials through the np to kick off, are further probed by computing atomic resolution stresses from nve simulations.Distribution of equilibrium atomistic stresses on the np reveals a largely asymmetric nature and suggests structural breathing may actually represent large dynamic changes in the hotspot regions, far from the np pores, which is in remarkable resemblance with recently conducted hydrogen-deuterium exchange coupled to mass spectrometry experiment.Communicated by ramaswamy h.Sarma.

Journal: Journal of biomolecular structure & dynamics (2022)
Publication types: Journal Article
A simple method for assessing rotational flexibility in adolescent idiopathic scoliosis: modified Adam's forward bending test.

This study aimed to evaluate the diagnostic aptitude of a modified adams forward bending test (mafbt), which addresses the coupling phenomenon of axial rotation with reference to the side-bending movement.Also, this evaluation was facilitated by the introduction of our rotational flexibility index (rfi).Methods thirty-two female and eight male ais patients were included in this study from a single institution.In the mafbt, subjects were asked to bend to the convex side of the curve in the forward bending position.Scoliometric measurements were done during the afbt and mafbt.Utilizing anteroposterior standing plain radiographs curve flexibility indices were calculated.The diagnostic aptitude of the mafbt was evaluated based on the receiver operating characteristic (roc) curves and area under the curve (auc).The rfi was also assessed, which considered afbt and mafbt parameters as a specified function.Results significant correlations were noted between the cobb angle and afbt (p = 0.005), fulcrum bending and the mafbt (p = 0.0001), side-bending and mafbt (p = 0.0001).There were significant positive correlations between rotational flexibility as based on fulcrum bending radiograph to that of the rfi (r = 0.4, p = 0.036) and side-bending technique (r = 0.4, p = 0.008).Based on roc analyses (auc range 0.7-0.8), the mafbt demonstrated high specificity and sensitivity rates for flexible and rigid curves, respectively.Conclusions this is the first study to report that the mafbt is a simple and reliable test for the clinical assessment of rotational flexibility in ais patients.The study further noted that the novel rfi has clinical utility in the assessment of ais.

Journal: Spine deformity (2021)
Publication types: Journal Article
Guidelines for Burr Hole Surgery in Combination With Tumor Treating Fields for Glioblastoma: A Computational Study on Dose Optimization and Array Layout Planning.

Tumor treating fields (ttfields) is an anti-cancer technology increasingly used for the treatment of glioblastoma.Recently, cranial burr holes have been used experimentally to enhance the intensity (dose) of ttfields in the underlying tumor region.In the present study, we used computational finite element methods to systematically characterize the impact of the burr hole position and the ttfields transducer array layout on the ttfields distribution calculated in a realistic human head model.We investigated a multitude of burr hole positions and layouts to illustrate the basic principles of optimal treatment planning.The goal of the paper was to provide simple rules of thumb for physicians to use when planning the ttfields in combination with skull remodeling surgery.Our study suggests a number of key findings, namely that (1) burr holes should be placed directly above the region of interest, (2) field enhancement occurs mainly underneath the holes, (3) the ipsilateral array should directly overlap the holes and the contralateral array should be placed directly opposite, (4) arrays in a pair should be placed at far distance and not close to each other to avoid current shunting, and finally (5) rotation arrays around their central normal axis can be done without diminishing the enhancing effect of the burr holes.Minor deviations and adjustments (<3 cm) of arrays reduces the enhancement to some extent although the procedure is still effective in these settings.In conclusion, our study provides simple guiding principles for implementation of dose-enhanced ttfields in combination with burr-holes.Future studies are required to validate our findings in additional models at the patient specific level.

Journal: Frontiers in human neuroscience (2022)
Publication types: Journal Article
Bladder infection with uropathogenicEscherichia coliincreases the excitability of afferent neurons.

Urinary tract infections (utis) cause bladder hyperactivity and pelvic pain, but the underlying causes of these symptoms remain unknown.We investigated whether afferent sensitization contributes to the bladder overactivity and pain observed in mice suffering from experimentally induced bacterial cystitis.Inoculation of mouse bladders with the uropathogenic escherichia colistrain uti89 caused pelvic allodynia, increased voiding frequency, and prompted an acute inflammatory process marked by leukocytic infiltration and edema of the mucosa.Compared with controls, isolated bladder sensory neurons from uti-treated mice exhibited a depolarized resting membrane potential, lower action potential threshold and rheobase, and increased firing in response to suprathreshold stimulation.To determine whether bacterial virulence factors can contribute to the sensitization of bladder afferents, neurons isolated from naïve mice were incubated with supernatants collected from bacterial cultures with or depleted of lipopolysaccharide (lps).Supernatants containing lps prompted the sensitization of bladder sensory neurons with both tetrodotoxin (ttx)-resistant and ttx-sensitive action potentials.However, bladder sensory neurons with ttx-sensitive action potentials were not affected by bacterial supernatants depleted of lps.Unexpectedly, ultrapure lps increased the excitability only of bladder sensory neurons with ttx-resistant action potentials, but the supplementation of supernatants depleted of lps with ultrapure lps resulted in the sensitization of both population of bladder sensory neurons.In summary, the results of our study indicate that multiple virulence factors released from uti89 act on bladder sensory neurons to prompt their sensitization.These sensitized bladder sensory neurons mediate, at least in part, the bladder hyperactivity and pelvic pain seen in mice inoculated with uti89.new & noteworthyurinary tract infection (uti) produced by uropathogenic escherichia coli(upec) promotes sensitization of bladder afferent sensory neurons with tetrodotoxin-resistant and tetrodotoxin-sensitive action potentials.Lipopolysaccharide and other virulence factors produced by upec contribute to the sensitization of bladder afferents in uti.In conclusion, sensitized afferents contribute to the voiding symptoms and pelvic pain present in mice bladder inoculated with upec.

Journal: American journal of physiology. Renal physiology (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural;Research Support, Non-U.S. Gov't