syedzayyan/paper_mill_benchmark huggingface dataset:
Antidiabetic Effect of Tamarindus indica and Momordica charantia and Downregulation of TET-1 Gene Expression by Saroglitazar in Glucose Feed Adipocytes and Their Involvement in the Type 2 Diabetes-Associated Inflammation In Vitro

To date, there is no satisfactory and effective therapy available to cure type 2 diabetes mellitus (t2dm).This present work is focused on plant extracts and the effect of saroglitazar and tet genes on oxidative stress and inflammation in vitro adipocytes.Aqueous extracts of tamarindus indica and momordica charantia seed have shown potent antidiabetic activity that decreases glucose levels in diabetic adipocytes.After seven and fourteen days, the sugar level in the blood was significantly reduced when plant extracts were supplemented.Lipid profiles including total cholesterol (tc), triglyceride (tgl), high‐density lipoprotein (hdl), low‐density lipoprotein (ldl), and very low‐density lipoprotein (vldl) showed a highly significant change as expected in adipocytes treated with glucose compared with controlled adipocytes ( p &lt; 0.001).Gene expression of catalase, superoxide dismutase (sod1, sod2), and glutathione peroxidase (gpx) are changed twice, thrice, and quadruplet, respectively.The level of interleukin‐1 (il1) and tumor necrosis factor‐ α (tnf‐ α ) was restored but the interleukin‐6 (il6) and ten‐eleven‐translocation‐1 (tet1) were completely knocked down by the use of saroglitazar.In comparison with the diabetic group, this supplementation significantly increased glycogen content and glucose‐6‐phosphate dehydrogenase activity.In the extract supplemented group, glucose‐6‐phosphatase, glucose‐oxidizing enzyme, and glucose‐phosphorylating enzyme activities were significantly reduced.After seven days of extract supplementation, these parameters were not resettled to a controlled level; however, after 14 days of supplementation, all parameters were restored to the control level.In addition to altering gene expression, tet enzymes may contribute to altered adiposity and its metabolic consequences.The purpose of this study is to examine new ideas and approaches for treating obesity, t2dm, and other associated metabolic disorders.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Ant Colony Optimization-Enabled CNN Deep Learning Technique for Accurate Detection of Cervical Cancer

Cancer is characterized by abnormal cell growth and proliferation, which are both diagnostic indicators of the disease.When cancerous cells enter one organ, there is a risk that they may spread to adjacent tissues and eventually to other organs.Cancer of the cervix of the uterus often initially manifests itself in the uterine cervix, which is located at the very bottom of the uterus.Both the growth and death of cervical cells are characteristic features of this condition.False‐negative results provide a significant moral dilemma since they may cause women to get an incorrect diagnosis of cancer, which in turn can result in the woman’s premature death from the disease.False‐positive results do not raise any significant ethical concerns; but they do require a patient to go through an expensive and time‐consuming treatment process, and they also cause the patient to experience tension and anxiety that is not warranted.In order to detect cervical cancer in its earliest stages in women, a screening procedure known as a pap test is often performed.This article describes a technique for improving images using brightness preserving dynamic fuzzy histogram equalization.To individual components and find the right area of interest, the fuzzy c‐means approach is applied.The images are segmented using the fuzzy c‐means method to find the right area of interest.The feature selection algorithm is the aco algorithm.Following that, categorization is carried out utilizing the cnn, mlp, and ann algorithms.

Journal: BioMed Research International (2023)
Publication types: Research Article;
Analysis of Multigene Mutations in Lung Adenocarcinoma in Zunyi

Driver gene mutation in lung adenocarcinoma patients in zunyi and its relationship with clinical features were probed in this investigation.Methods .In total, with 244 patients with lung adenocarcinoma as study subjects, including 141 males and 103 females, amplification‐refractory mutation system‐polymerase chain reaction (arms‐pcr) was utilized for detecting multigene mutations.Subsequently, the relationship between gene mutation and clinical characteristics was analyzed.Results .The total mutation rate of driver genes was 65.17%, including 48.36% egfr, 6.15% kras, 5.74% alk, 2.05% her‐2, 1.23% ros1, 0.82% ret, 0.41% nras, and 0.41% braf.Among egfr mutations, 47.46% were egfr‐19‐deletion, 42.37% egfr‐21‐l858r mutation, 4.24% egfr‐20‐t790m mutation, 2.54% egfr‐21‐l861q mutation, 2.54% egfr‐20‐insertion, and 0.85% egfr‐18‐g719x mutation.Both female patients and nonsmoking patients with lung adenocarcinoma had a higher rate of egfr mutation.Additionally, 15 patients with multiple mutations in egfr, including 13 patients with 2 mutations in egfr and 2 patients with 3 mutations in egfr, were found.Conclusion .Among driver gene mutations in patients with lung adenocarcinoma in zunyi, egfr mutation has the highest incidence, followed by alk fusion and kras mutation.Although both mutations and multisite mutations in the other driver genes account for a low proportion, they still have great clinical significance.Multigene mutation detection contributes to the rapid screening of patients with lung adenocarcinoma who respond to targeted therapy.

Journal: BioMed Research International (2021)
Publication types: Research Article;
Analysis of Molecular Mechanism of YiqiChutan Formula Regulating DLL4-Notch Signaling to Inhibit Angiogenesis in Lung Cancer

In order to explore the specific mechanism of yiqi chutan formula (yqctf) in inhibiting the angiogenesis of lung cancer and its relationship with delta‐like ligand 4‐ (dll4‐) notch signaling, 30 healthy balb/c‐nu/nu rats were selected and divided into three groups: a549 group (implanted with lung adenocarcinoma cell line a549), nci‐h460 group (implanted with human lung large‐cell carcinoma cell line nci‐h460), and nci‐h446 group (implanted with human lung small cell carcinoma cell line nci‐h446) for constructing lung cancer transplanted tumor models.After modeling, the group treated with normal saline was taken as control group, 200 mg/kg of yqctf was adopted for intervention, and the tumor volume and growth inhibition rate were compared with the vascular targeted inhibitor sorafenib.He staining, cd31 fluorescent antibody staining, and microelectron microscopy were adopted to observe the neovascular endothelial cells of the transplanted tumor.The expression of vegf, hif‐1 α , dll4, and notch‐1 in the transplanted tumors in each group was detected by western blot and rt‐pcr at the protein level or m rna level.Compared with the control group, the yqctf‐treated group had obvious inhibitory effect on lung cancer transplanted tumor and lung cancer angiogenesis.In the yqctf‐treated group, the density of angiogenesis decreased significantly and the vascular lumen structure also decreased, and the expression levels of vegf, hif‐1 α , dll4, and notch‐1 in the yqctf‐treated group were all lower than those in the control group.Yqctf could inhibit the growth of lung cancer transplanted tumor through antiangiogenesis, and it could also reduce the amount of angiogenesis in lung cancer transplanted tumor.In addition, the generation of lumen structure was also hindered, which was realized through the vegf signaling pathway and dll4‐notch signaling pathway.

Journal: BioMed Research International (2021)
Publication types: Research Article;
Conversion of Aquaculture Waste into Biomedical Wealth: Chitin and Chitosan Journey

Biowaste originating from aquaculture sector represents a potential feedstock to produce value-added substances and materials like chitin and chitosan.They are the long chain polymers of n-acetylglucosamine polymers with huge industrial and biomedical significance.Chitin has long been recognized as a useful biomaterial for drug delivery and neurological therapy.Similarly, chitosan oligosaccharide, a short chain polymer derived from chitin/chitosan, has been identified for its potential biomedical applications, including antibacterial, anti-inflammatory, and anti-alzheimer action.Chitosan nanoparticles are also used extensively in biomedical applications.Here we have critically summarized various methods for the extraction of chitin, chitosan, and chitooligossacharides and chitosan nanoparticle preparation and their diverse biomedical applications.

Journal: Advances in Materials Science and Engineering (2022)
Publication types: Research Article;
Appropriate Supervised Machine Learning Techniques for Mesothelioma Detection and Cure

Mesothelioma is a dangerous, violent cancer, which forms a protecting layer around inner tissues such as the lungs, stomach, and heart.We investigate numerous ai methodologies and consider the exact dm conclusion outcomes in this study, which focuses on dm determination.K‐nearest neighborhood, linear‐discriminant analysis, naive bayes, decision‐tree, random forest, support vector machine, and logistic regression analyses have been used in clinical decision support systems in the detection of mesothelioma.To test the accuracy of the evaluated categorizers, the researchers used a dataset of 350 instances with 35 highlights and six execution measures.Lda, nb, knn, svm, dt, log r, and rf have precisions of 65%, 70%, 92%, 100%, 100%, 100%, and 100%, correspondingly.In count, the calculated complication of individual approaches has been evaluated.Every process is chosen on the basis of its characterization, exactness, and calculated complications.Svm, dt, log r, and rf outclass the others and, unexpectedly, earlier research.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Construction and Simulation of the Enterprise Financial Risk Diagnosis Model by Using Dropout and BN to Improve LSTM

In view of the financial risks faced by listed enterprises, how to accurately predict the risks is an important work.However, the traditional lstm financial diagnosis model has the disadvantage of low accuracy; the specific reason is that the lstm model has the problems of overfitting and gradient disappearance in risk diagnosis.Therefore, dropout is adopted to solve the overfitting problem in the process of premodel prediction, and the bn algorithm is used to solve the gradient disappearance problem in the process of iteration.In order to verify the feasibility of above improvements, the financial data of china’s a-share listed enterprises from 2017 to 2020 are taken as samples to analyze the financial data of listed enterprises through single-step dimension and multistep dimension.The experimental results show that under the analysis of two dimensions, the financial prediction accuracy of the improved lstm for t-2∼t-3 years can reach 83.96% and 91.19%, respectively, which indicates that through the above improvements, the model can be improved and has certain reference value.

Journal: Security and Communication Networks (2022)
Publication types: Research Article;
Analysis of Intestinal Flora and Levels of Epidermal Growth Factor Receptor, Interleukin-32, and Gastrin 17 in Patients with Gastric Cancer via Carbon Nanoparticle Laparoscopy

In order to explore the changes of intestinal flora and serum levels of relevant substances in patients with gastric cancer before and after surgery with carbon nanoparticle laparoscopy, a total of 180 patients with early distal gastric cancer who adopted laparoscopic radical gastrectomy for distal gastric cancer in the general surgery department of tcm hospital of shi jia zhuang city from january 2018 to january 2020 were selected and randomly divided into two groups: traditional laparoscopic operation (control group) and carbon nanoparticle laparoscopic operation (experimental group) were adopted for treatment for the two groups, respectively.Postoperative evaluation included the difference between the two groups in the operative time, the efficiency of intraoperative lymph node dissection, and the number of lymph node detection.The adverse reactions, changes of intestinal flora before and after surgery in the two groups, and the serum levels of epidermal growth factor receptor (egfr), interleukin‐32 (il‐32), and gastrin 17 were evaluated.In the experimental group, the success rate of carbon nanoparticle tracer black staining reached 100%, and the operation time of the experimental group was significantly shorter than that of the control group ( p &lt; 0.05).The lymph node detection rate of the experimental group was higher than that of the control group ( p &lt; 0.05), but there was no significant difference in the lymph node metastasis rate between the two groups ( p &gt; 0.05).The sentinel lymph node sensitivity of the experimental group reached 92.3%, and the specificity, accuracy, and positive and negative prediction rates reached 100%; the experimental group patients were with an obviously higher incidence of level i‐ii gastrointestinal reaction ( p &lt; 0.05).Postoperative increases in bifidobacteria and lactobacillus were observed in both groups, while decreases in enterococcus and escherichia coli were observed in both groups ( p &lt; 0.05).Moreover, the degree of increase and decrease in the experimental group was greater than that in the control group ( p &lt; 0.05).The serum levels of egfr, il‐32, and gastrin 17 in the two groups were significantly lower than those in the control group on 3 d, 7 d, and 15 d after surgery ( p &lt; 0.05).In the radical gastrectomy for distal gastric cancer, carbon nanoparticle laparoscopy was not only helpful for the localization of small tumors but also for the thorough dissection of lymph nodes after the surgery, and the postoperative adverse reactions of carbon nanoparticle laparoscopy were also less, which was of great significance for the improvement of intestinal flora and the reduction of serum levels of egfr, il‐32, and gastrin 17 in gastric cancer patients.

Journal: BioMed Research International (2021)
Publication types: Research Article;
Analysis of Development Mechanism of Giant Cell Arteritis in Nude Mouse Model through Color Duplex Sonography and Computerized Tomography Nanocontrast Agent

To explore the application value of color duplex sonography and enhanced computerized tomography (ct) inspection based on a nanocontrast agent in diagnosis and pathogenesis in giant cell arteritis (gca), the gca nude mouse model was constructed.In this study, 40 healthy male bal b/c nude mice aged 6‐8 weeks were randomly divided into a control group (no model) and an experimental group (model), with 20 mice in each group, and the temporal artery tissue of gca patients diagnosed as positive by temporal artery biopsy was implanted into nude mice to construct a gca nude mouse model.Abdominal aortic biopsy and immunohistochemistry were used to verify the success of the gca nude mouse model.All nude mice were subjected to color duplex sonography and enhanced ct examination based on a nanocontrast agent.At the same time, the basic indicators such as body weight, temperature, white blood cell (wbc), lymphocytes (lym), hemoglobin (hgb), and platelet (plt) were measured, and the protein expression levels of interleukin‐6 (il‐6) and signal transducer and activator of transcription 3 (stat3) were detected by immunohistochemistry.The results showed that the temporal artery wall of the nude mice in the experimental group thickened and the lumen was significantly narrowed, indicating that the cell arteritis model of nude mice was successfully constructed; ultrasound examination showed that the right superficial temporal artery vascular cavity narrowed, the blood flow signal changed like a filling defect around the periphery, and there was a low echo halo.Ct examination showed that the left superficial temporal artery narrowed, and the inner diameter of the narrow segment of blood vessels changed like a bead.The body weight of nude mice in the experimental group decreased significantly after the modeling was completed ( p &lt; 0.05); after modeling, the body temperature of the nude mice in the experimental group increased significantly ( p &lt; 0.05); lym and hgb values of nude mice in the experimental group were significantly lower than those in the control group ( p &lt; 0.05); the content of il‐6, stat3, il‐6, and stat3 proteins in the arterial tissue of nude mice in the experimental group was lower than that of the control group ( p &lt; 0.05), indicating that color duplex sonography and ct contrast agent technology can be used in the diagnosis and development mechanism research of gc.

Journal: BioMed Research International (2021)
Publication types: Research Article;
Analysis of Antiapoptosis Effect of Netrin-1 on Ischemic Stroke and Its Molecular Mechanism under Deleted in Colon Cancer/Extracellular Signal-Regulated Kinase Signaling Pathway

To analyze the regulatory effect of netrin‐1 in ischemic stroke and its influence on deleted in colon cancer (dcc)/extracellular signal‐regulated kinase (erk) signaling pathway, 20 male rats were selected to construct the rat model of middle cerebral artery occlusion (mcao), 10 normal rats were selected as healthy controls (normal saline (ns)), and they were divided into the mcao+netrin‐1 group, mcao group, and ns group according to different treatment schemes.The positive expression of netrin‐1 was detected by immunostaining, magnetic resonance imaging (mri) was adopted to detect the percentage of rat cerebral infarct volume in the cerebral hemispheres, and modified neurological severity score (m nss) was adopted to evaluate postoperative neurological function in rats.Besides, a tunnel staining experiment was applied to detect the apoptosis rate of rat neurons, the sticker removal test was applied to evaluate the postoperative sensory function of rats, and fluorescence staining was adopted to detect the expression of dcc and erk in rats.The results showed that the percentage of cerebral infarction volume in the cerebral hemispheres of the mcao+netrin‐1 group was higher than that of the mcao and ns groups ( p &lt; 0.05); in the mcao+netrin‐1 group, the mcao m nss scoring and the time spent in the sticker removal test were lower than the mcao group ( p &lt; 0.05); the apoptosis rate of rats in the mcao+netrin‐1 group was lower than that in the mcao group ( p &lt; 0.05); the average fluorescence intensity of dcc and p‐erk in the mcao+netrin‐1 group was higher than that in the mcao group ( p &lt; 0.05); the average fluorescence intensity of p‐erk in the mcao+netrin‐1 group was higher than that in the mcao group ( p &lt; 0.05).In short, netrin‐1 can effectively reduce the brain tissue damage in rats with ischemic stroke, improve the nerve function and sensory function of rats, and inhibit neuronal cell apoptosis.Netrin‐1 can promote dcc expression and erk phosphorylation, and the epk signaling pathway may be involved in the antiapoptotic effect of netrin‐1.

Journal: BioMed Research International (2020)
Publication types: Research Article;
Application Experience and Patient Feedback Analysis of 3D Printed AFO with Different Materials: A Random Crossover Study

This study is aimed at analyzing the application experience and feedback of the patients with poststroke ankle dorsiflexion disorders for 3d printed afo with three different materials.Methods .15 patients were randomly divided into three groups; 3d printed afo with 3 different materials (pa2200, somos ne xt, and pa12) was used to each group, according to the crossover study design, in order to ask the three groups of patients to use three different materials of 3d printed afo.Assessment was taken by the end of each test round.Through statistical processing, the patient feedback data of the three groups of materials of 3d printed afo were obtained.Results .In the material comfort assessment of the afo, somos ne xt was compared with pa2200, and the p value was &lt;0.05; in the item of surface smoothness of the afo, somos ne xt was compared with pa2200, and the p value was &lt;0.01; at the same time, pa12 was compared with pa2200, and the p value was &lt;0.05.Conclusion .The 3 different materials of 3d printing afo bring different experience, and we also have sufficient reason to believe that there will be differences in the auxiliary effect of this on patients, which leads the patient’s selection too.The material somos ne xt is much popular and has certain clinical advantages.

Journal: BioMed Research International (2021)
Publication types: Research Article;
CD4-Targeted T Cells Rapidly Induce Remissions in Mice with T Cell Lymphoma

To explore the immune cell therapy for t cell lymphoma, we developed cd4‐specific chimeric antigen receptor‐ (car‐) engineered t cells (cd4cart), and the cytotoxic effects of cd4cart cells were determined in vitro and in vivo.Methods .Cd4cart cells were obtained by transduction of lentiviral vector encoding a single‐chain antibody fragment (sc fv) specific for cd4 antigen, costimulatory factor cd28 fragment, and intracellular signal transduction domain of cd3 fragments.Control t cells were obtained by transduction of reporter lentiviral vector.The cytotoxicity, tumor growth, and survival rate of mice with t cell lymphoma were analyzed after adoptive t cell transfer in vivo.Results .Cd4cart cells had potent cytotoxic activity against cd4+ t1301 tumor t cells in a concentration‐dependent manner.In addition, adoptive cd4cart cell transfer significantly suppressed tumor growth and improved animal survival with t cell lymphoma, compared to the mice who received control t cells and pbs.Conclusion .Cd4cart cells have potent cytotoxic effects on t cell lymphoma.The study provided an experimental basis for cd4cart‐mediated therapy of t cell lymphoma.

Journal: BioMed Research International (2021)
Publication types: Research Article;