syedzayyan/paper_mill_benchmark huggingface dataset:
ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2-Beclin 1 Complex in Cervical Cancer Ca Ski Cells.

Rce-4, a steroidal saponin isolated from reineckia carnea, has been studied previously and has exhibited promising anti-cervical cancer properties by inducing programmed cell death (pcd) of ca ski cells.Considering the cancer cells developed various pathways to evade chemotherapy-induced pcd, there is, therefore, an urgent need to further explore the potential mechanisms underlying its actions.The present study focused on targeting the bcl-2-beclin 1 complex, which is known as the key regulator of pcd, to deeply elucidate the molecular mechanism of rce-4 against cervical cancer.The effects of rce-4 on the bcl-2-beclin 1 complex were investigated by using the co-immunoprecipitation assay.In addition, autophagy-related genes (atg) were also analyzed due to their special roles in pcd.The results demonstrated that rce-4 inhibited the formation of the bcl-2-beclin 1 complex in ca ski cells via various pathways, and atg 4b proteins involved in this process served as a key co-factor.Furthermore, based on the above, the sensitivity of rce-4 to ca ski cells was significantly enhanced by inhibiting the expression of the atg 4b by applying the atg 4b si rna plasmid.

Journal: International journal of molecular sciences (2021)
Publication types: Journal Article
Electrophysiological effects and clinical utility of propafenone in children.

This retrospective case series study sought to describe the safety and clinical effectiveness of propafenone for the control of arrhythmias in children with and without chd or cardiomyopathy.Methods we reviewed baseline characteristics and subsequent outcomes in a group of 63 children treated with propafenone at 2 sites over a 15-year period therapy was considered effective if no clinically apparent breakthrough episodes of arrhythmias were noted on the medication.Results sixty-three patients (29 males) were initiated on propafenone at a median age of 2.3 years.Chd or cardiomyopathy was noted in 21/63 (33%).There were no significant differences between demographics, clinical backgrounds, antiarrhythmic details, side effect profiles, and outcomes between children with normal hearts and children with chd or cardiomyopathy.Cardiac depression at the initiation of propafenone was more common amongst children with chd or cardiomyopathy compared to children with normal hearts.Systemic ventricular function was diminished in 15/63 patients (24%) prior to starting propafenone and improved in 8/15 (53%) of patients once better rhythm control was achieved.Other than one child in whom medication was stopped due to gastroesophageal reflux, no other child experienced significant systemic or cardiac side effects during treatment with propafenone.Propafenone achieved nearly equal success in controlling arrhythmias in both children with normal hearts and children with congenital heart disease or cardiomyopathy (90% versus 86%, p = 0.88).Conclusion propafenone is a safe and effective antiarrhythmic medication in children.

Journal: Cardiology in the young (2022)
Publication types: Journal Article
Factors influencing thigh muscle volume change with cycling exercises in acute spinal cord injury - a secondary analysis of a randomized controlled trial.

To conduct a per-protocol analysis on thigh muscle volume outcomes from the spinal cord injury and physical activity (scipa) switch-on trial.design:secondary analysis from an assessor-blind randomized, controlled trial.setting:four acute/sub-acute hospitals in australia and new zealand.participants:24 adults (1 female) within four weeks of motor complete or incomplete spinal cord injury (sci)intervention:functional electrical stimulation-assisted cycling (fesc) or passive cycling (pc) 4x/week for 12 weeks.outcome measures:whole thigh and muscle group volumes calculated from manually segmented mr images.results:19/24 participants completed ≥ twelve weeks of the intervention.Five participants experienced hypertrophy (4 fesc; 1 pc) and eight attenuation of atrophy ( 20% (3 fesc; 3 pc).Mean (sd) change for fesc was -2.3% (25.3%) and pc was -14.0% (12.3%).After controlling for baseline muscle volumes, a strong significant correlation was found between mean weekly exercise frequency and quadriceps and hamstring volumes (r=6.25, p=0.006), regardless of mode.Average watts was highly correlated to quadriceps volumes only (r=5.92, p=0.01), while total number of sessions was strongly correlated with hamstring volumes only (r=5.91, p=0.01).conclusion:this per-protocol analysis of fesc and pc early after sci reports a partial response in 42% and a beneficial response in 25% of patients who completed 12 weeks intervention, regardless of mode.Strong correlations show a dose-response according to exercise frequency.Characteristics of non-responders are discussed to inform clinical decision-making.

Journal: The journal of spinal cord medicine (2022)
Publication types: Journal Article;Randomized Controlled Trial;Research Support, Non-U.S. Gov't
Glucose protects the cell membrane, Na+/K+-ATPase, nucleic acids, and proteins in Bacillus subtilis spores under high pressure thermal sterilization.

The extreme resistance of bacterial spores to sterilization makes them a major concern to the food industry and consumers.In this study, the effect of glucose on the inactivation of bacillus subtilis spores by high pressure thermal sterilization (hpts) was evaluated.The results showed that the protective effects of glucose increased with the increase in its concentration.Compared with the hpts control (no addition of glucose), the activity of na+/k+-atpase was increased, the leakage of proteins and the release of 2,6-pyridine dicarboxylic acid (dpa) was decreased, and the vibrational strength of the functional group p = o was reduced by the addition of glucose.At the same time, glucose treatment increased the content of α-helix by 6%-22%, while decreased the random coil content by 5%-13% of the cellular protein.In conclusion, the addition of glucose protected the cell membrane, na+/k+-atpase, cellular nucleic acids and proteins of b.Subtilis under hpts treatment.

Journal: FEMS microbiology letters (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Rationalizing heptadecaphobia: TH17 cells and associated cytokines in cancer and metastasis.

Cancer is one of the leading causes of death worldwide.When cancer patients are diagnosed with metastasis, meaning that the primary tumor has spread to at least one different site, their life expectancy decreases dramatically.In the past decade, the immune system ́s role in fighting cancer and metastasis has been studied extensively.Importantly, immune cells and inflammatory reactions generate potent antitumor responses but also contribute to tumor development.However, the molecular and cellular mechanisms underlying this dichotomic interaction between the immune system and cancer are still poorly understood.Recently, a spotlight has been cast on the distinct subsets of immune cells and their derived cytokines since evidence has implicated their crucial impact on cancer development.T helper 17 cell (th17) cells, which express the master transcriptional factor retinoic acid-receptor-related orphan receptor gamma t, are among these critical cell subsets and are defined by their production of type 3 cytokines, such as il-17a, il-17f, and il-22.Depending on the tumor microenvironment, these cytokines can also be produced by other immune cell sources, such as t cytotoxic 17 cell, innate lymphoid cells, nkt cells, or γδ t cells.To date, a lot of data have been collected describing the divergent functions of il-17a, il-17f, and il-22 in malignancies.In this comprehensive review, we discuss the role of these th17- and non-th17-derived type 3 cytokines in different tumor entities.Furthermore, we will provide a structured insight into the strict regulation and subsequent downstream mechanisms of these cytokines in cancer and metastasis.

Journal: The FEBS journal (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't;Review
Do expectations of recovery improve risk assessment for people with whiplash-associated disorders? Secondary analysis of a prospective cohort study.

Whip predict, which includes prognostic factors of pain-related disability, age and hyperarousal symptoms, was developed and validated for prediction of outcome in people with whiplash associated disorders (wad).Patient expectations of recovery was not an included factor, though is known to mediate outcomes.The aim of this study was to determine whether the addition of expectations of recovery could improve the accuracy of whip predict.Methods two hundred twenty-eight participants with acute wad completed questionnaires (whip predict and expectations of recovery) at baseline.Health outcomes (neck disability index (ndi) and global perceived recovery (gpr)) were assessed at 6- and 12-months post injury.Cut-off points for expectations of recovery predictive of both full recovery (ndi ≤10 % , gpr ≥ 4) and poor outcome (ndi ≥30 % , gpr ≤ - 3) were determined, and multivariate logistic regression analyses were used to compare models with and without this variable.Results expectations of recovery improved or maintained the accuracy of predictions of poor outcome (6-months: sensitivity 78 to 83%, specificity maintained at 79.5%; 12-months: sensitivity maintained at 80%, specificity 69 to 73%).The sensitivity of predictions of full recovery improved (6-months: 68 to 76%; 12-months: 57 to 81%), though specificity did not change appreciably at 6 months (80 to 81%) and declined at 12 (83 to 76%).Roc curves indicated a larger and more consistent improvement in model performance when expectations of recovery were added to the pathway predictive of full recovery.Conclusions the addition of expectations of recovery may improve the accuracy of whip predict, though further validation is required.

Journal: BMC musculoskeletal disorders (2022)
Publication types: Journal Article
The Power of Systems Biology: Insights on Lupus Nephritis from the Accelerating Medicines Partnership.

The accelerating medicines partnership (amp) sle network united resources from academic centers, government, nonprofit, and industry to accelerate discovery in lupus nephritis (ln).The amp sle network developed a set of protocols for high-throughput analyses to systematically study kidney tissue, urine, and blood in ln.This article summarizes approaches and results from phase 1 of amp sle network effort, including single cell rna-seq analysis of ln kidney biopsies, cellular and proteomic studies of ln urine, and mass cytometry immunophenotyping of blood cells.This work provides a framework to guide studies of the clinical implications of active cellular/molecular pathways in ln.

Journal: Rheumatic diseases clinics of North America (2021)
Publication types: Journal Article;Research Support, N.I.H., Extramural;Research Support, Non-U.S. Gov't;Review
A prospective evaluation of diagnostic performance of a combo rapid antigen test QuickNavi-Flu+COVID19 Ag.

Introduction since respiratory sample collection is an uncomfortable experience, simultaneous detection of pathogens with a single swab is preferable.We prospectively evaluated the clinical performance of a newly developed antigen test quick navi-flu+covid19 ag (denka co., ltd., tokyo, japan) which can detect severe acute respiratory syndrome coronavirus 2 (sars-co v-2) and influenza viruses at the same time with a single testing device.Methods we included those who were suspected of contracting coronavirus disease 2019 (covid-19) and were referred to a pcr center at ibaraki prefecture in japan, between august 2, 2021 to september 13, 2021, when the variant carrying l452r spike mutation of sars-co v-2 were prevalent.Additional nasopharyngeal samples and anterior nasal samples were obtained for the antigen test and were compared with a reference real-time reverse transcription pcr (rt-pcr) using nasopharyngeal samples.Results in total, 1510 nasopharyngeal samples and 862 anterior nasal samples were evaluated.During the study period, influenza viruses were not detected by quick navi-flu+covid19 ag and reference real-time rt-pcr.For sars-co v-2 detection in nasopharyngeal samples, the sensitivity and specificity of the antigen test were 80.9% and 99.8%, respectively.The sensitivity and specificity using anterior nasal samples were 67.8% and 100%, respectively.In symptomatic cases, the sensitivities increased to 88.3% with nasopharyngeal samples and 73.7% with anterior nasal samples.There were three cases of discrepant results between the antigen test and the real-time rt-pcr.All of them were positive with the antigen test but negative with the real-time rt-pcr in sars-co v-2 detection.Conclusion a combo kit, quick navi-flu+covid19 ag, showed an acceptable sensitivity and sufficient specificity for sars-co v-2 detection, especially using nasopharyngeal sample collected from symptomatic patients.

Journal: Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy (2022)
Publication types: Journal Article
Attitudes towards Substance Use Disorders among Students in the Social Sciences: The Role of Gender and Terminology.

Negative attitudes towards individuals with substance use disorders (suds) have been consistently reported within the general population, as well as among health professionals and medical students.objectives:to explore attitudes towards suds held by students in the social sciences, the future-to-be mental health professionals, as well as factors, which may affect these attitudes.methods:undergraduate and graduate students in the social sciences (psychology, social work, criminology;n= 184), were presented a vignette describing an individual with substance-related problems.Participants were randomly assigned with one of two vignettes, either addressing him as "suffering from substance use disorder" or as an "addict." attitudes were assessed by exploring the degree to which participants perceive the described individual as posing social threat and in need of treatment.results:women generally perceived individuals with suds as in more need for treatment compared to men.Men, but not women, were significantly affected by the terminology used, with less negative attitudes reported when using medical terminology.conclusion:gender differences in attitudes towards individuals with suds may be present in earlier stages of mental health professionals' career.More emphasis should be given on the use of non-stigmatizing terminology among students in the social sciences, particularly men.

Journal: Substance use & misuse (2020)
Publication types: Journal Article
Multimodal Imaging-Guided Photothermal Immunotherapy Based on a Versatile NIR-II Aggregation-Induced Emission Luminogen.

Synergistic photothermal immunotherapy has captured great attention owing to the mutually strengthening therapeutic outcomes towards both original tumors and abscopal tumors.Herein, a versatile theranostic agent displaying aggregation-induced emission, namely tpa-bt-dptq, was designed and prepared based on benzo[c]thiophene unit as a building block; it can be used for simultaneous fluorescence imaging (fli) in the second near-infrared (nir-ii) window, photoacoustic imaging (pai), photothermal imaging (pti), and thermal eradication of tumors.Further experiments validate that photothermal therapy (ptt) mediated by tpa-bt-dptq nanoparticles not only destroys the primary tumor but also enhances immunogenicity for further suppressing the growth of tumors at distant sites.Furthermore, ptt combining a programmed death-ligand 1 (pd-l1) antibody prevents the metastasis and recurrence of cancer by potentiating the effect of immunotherapy.

Journal: Angewandte Chemie (International ed. in English) (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
A 0.66mW 400 MHz/900 MHz Transmitter IC for In-Body Bio-Sensing Applications.

A sub-1ghz transmitter (tx) integrated chip (ic) with ultra-low power consumption and moderately high adjacent channel power rejection (acpr) is presented for in-body bio-sensing applications.The 400 mhz 12-phase digital power amplifier (dpa) is implemented with the proposed 16qam modulation scheme to improve the energy efficiency.The tx ic also contains a 900 mhz fsk tx realized with a symmetrical edge-combiner, which can be used in the low accuracy mode.A fully digital modulator with band shaping is integrated on the chip for the improvement of acpr performance.Fabricated in 65-nm cmos process, the chip occupies an active area of 0.75 mm2.Under 0.5 v supply voltage, the tx consumes less than 0.66 m w power consumption while delivering -15 d bm of output power when operating at both bands.The presented tx has an energy efficiency performance comparable to the state-of-the-arts low power designs, with the measured average energy consumption of 64.5/220 p j/bit, and the measured figure-of-merit (fo m) of 2.04/6.98 n j/(bit · m w) for the two bands.Compared with the state-of-the-arts sub-1m w designs in literatures, the acpr is improved by at least 13 d b.

Journal: IEEE transactions on biomedical circuits and systems (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Are the levels of uric acid associated with biometeorological conditions?

Hyperuricemia is an independent risk factor for renal and cardiovascular diseases and is closely associated with gout episodes.It is caused, inter alia, by nutritional habits and genetic factors, and also displays seasonal variability conditioned by meteorological factors.The impact of meteorological factors, including both cold and heat stress, on the human physiology is presented based on the universal thermal climate index (utci) - a biometeorological index derived from an analysis of human thermal balance.The aim of our study was to establish whether seasonal variations significantly affect routinely measured urine acid (ua) levels and could eventually support the clinical decision making process, as well as assessing whether utci values are correlated with ua levels in blood serum.This work presents a retrospective epidemiological study of data collected in olsztyn (poland).Study material comprised 54,536 results of ambulatory tests measuring ua levels, performed during the period 2016-2019.The analysis concerned correlations between ua and the ages of female and male subjects as well as existing biometeorological conditions as represented by utci values in an annual cycle.Ua levels in females were found to be lower (4.94 ± 1.37 sd) as compared to those of males (6.13 ± 1.43 sd) and demonstrated a strong positive correlation with age.Ua values differed significantly (p < 0.05) on days characterized by cold stress and heat stress, for the oldest age group.Ua levels were found to differ depending on the season, but these relationships were not statistically significant, except for significantly higher ua levels in females in autumn (p < 0.001).However, there was an evident difference in population ua levels under cold stress conditions (lower) and heat stress conditions (higher) in the elderly.The utci is an adequate predictor of population variations in ua levels since it takes into account the variability of local meteorological conditions.

Journal: The Science of the total environment (2022)
Publication types: Journal Article
Healthcare inequality in compensation claims concerning acute hospital services: a Danish register-based study.

Although citizens' equal right to acute healthcare of appropriate quality is an oft-cited goal for modern societies, healthcare disparities may persist.We aimed to investigate inequality in compensation claims and compensation payments regarding acute healthcare services.Design and setting we conducted a cross-sectional study of compensation claim patterns using the danish patient compensation association (dpca) registries.Participants, interventions and main outcome measures we used register data on all cases managed by dpca relating to acute hospital healthcare for adults (aged > 18 years) from 2007 to 2017.Results in total, the dpca had 5556 compensation claims for injuries caused by acute care services during the years 2007-2017.Age group of 50-64 years (odds ratio (or) = 1.37 compared with those aged 18-49 years; p < 0.001), marriage (or = 1.14; p < 0.001), higher income (or = 1.55; p < 0.001) and danish origin (or = 1.49; p < 0.001) were statistically associated with higher odds for filing a compensation claim; men (or = 0.83; p < 0.001) and those with many co-morbidities were much less represented (or = 0.24; p < 0.001).Male gender (or = 1.25; p < 0.001) and higher age (or = 2.55 (80+ years); p < 0.001) were associated with higher odds for a compensation award.Failed diagnosis was also more often at stake in men (or = 1.38; p < 0.001) and in patients aged 50-64 years (or = 1.17; p < 0.001) but occurred less often in patients with multiple morbidities (or = 0.68; p < 0.001).Conclusions findings from our danish material suggest some inequality in compensation claims and compensation payments regarding acute healthcare services.

Journal: International journal for quality in health care : journal of the International Society for Quality in Health Care (2021)
Publication types: Journal Article
Prevalence of sleep disturbance and the association between poor disease control in people with ankylosing spondylitis within the Australian clinical setting (ASLEEP study): a real-world observational study using the OPAL dataset.

Introduction sleep disturbance and fatigue are commonly reported in ankylosing spondylitis (as) but specific prevalence and the relationship to disease control are unknown.Method this retrospective non-interventional observational study of data from the opal dataset included patients with as (icd code m45, m45.0 or m08.1), aged 18 to 95 years and had completed ≥ 1 sleep questionnaire between 1 january 2019 and 30 september 2020.The prevalence of insomnia and obstructive sleep apnoea were assessed using the insomnia severity index (isi) and multivariate apnoea prediction index (mapi), respectively.Propensity score (ps) matching based on sex, age and symptom duration increased comparability between patients administered tumour necrosis factor inhibitors (tnfi) and interleukin 17a inhibitors (il-17ai).Results four hundred ninety-five patients were included.The mean isi total score in the overall population was 8.6 ± 6.2.Self-reported moderate or severe clinical insomnia was present in 16% and 3.2% of patients, respectively.The mean mapi score was 0.4 ± 0.3, self-reported apnoea was identified in 31.5% of patients and the mean facit-fatigue score was 36.1 ± 10.7.In the ps matched population, the only treatment-related difference was the mean mapi score (il-17ai 0.4 ± 0.3 and tnfi 0.3 ± 0.2, p = 0.046).Those with poor disease control (basdai ≥ 4) were more likely (odds ratio [or] 7.29, 95% ci 2.37 to 22.46, p = 0.001) to have a greater severity of insomnia symptoms than those with good disease control.Conclusion in this real-world as cohort, poor disease control was associated with sleep disturbance.Little difference in sleep disturbance was observed between biologic tnfi and il-17ai treatment.Key points • sleep disturbance and fatigue are common in patients with ankylosing spondylitis.• in our real-world cohort, self-reported apnoea was reported in one-third of patients; and one in five patients reported moderate to severe insomnia.• those with poor disease control were more likely to experience greater sleep disturbance than those with good disease control.

Journal: Clinical rheumatology (2022)
Publication types: Journal Article;Observational Study