syedzayyan/paper_mill_benchmark huggingface dataset:
Anti-inflammatory effect of flavonoids from chestnut flowers in lipopolysaccharide-stimulated RAW 264.7 macrophages and acute lung injury in mice.

Ethnopharmacological relevance chestnut flowers were one of the by-products during chestnut industrial processing.Chestnut (castanea mollissima blume) flower is rich in flavonoids and has been used as a traditional medicine to treat a variety of diseases including respiratory disorders for a long history.Aim of the study the present study aims to investigate the potential anti-inflammatory effect of flavonoids from chestnut flower (fcf) in lipopolysaccharide (lps)-treated raw 264.7 cells and stimulated acute lung injury (ali) in mice.Materials and methods hplc-esi-ms/ms was applied to identify flavonoids from chestnut flower.The ros content in cells and lung tissue was measured by flow cytometry.The malondialdehyde (mda) content, superoxide dismutase (sod) activity and glutathione (gsh) content in cells and bronchoalveolar lavage fluid (balf) was analyzed by photometry.Furthermore, the level of pro-inflammatory factors was analyzed by elisa, and the expression of inflammatory gene m rna by fluorescence quantitative pcr.H&e staining was used to evaluate the degree of lung tissue injury in mice.Mpo activity was used to measure the degree of neutrophil infiltration.Total protein content was detected by bca method.Results a total of forty-nine flavonoids compounds were tentatively identified in fcf by mass spectrometry analysis.The results of cell experiment suggested that fcf could alleviate oxidative injury via increasing sod activity and gsh content, as well as inhibiting the production of intracellular ros and mda.Fcf exerted its protective effect by suppressing the expression of both inducible nitric oxide synthase (i nos) and cycooxygenase 2 (cox-2) to inhibit the synthesis of pro-inflammatory factors and cytokines, including no, pge2, tnf-α, il-6 and il-1β.Besides, fcf treatment could alleviate the thickening of alveolar wall and pulmonary congestion in lps-treated ali mice, and significantly inhibit the activity of myeloperoxidas (mpo) and the expression of cytokines in balf.Conclusions fcf could ameliorate inflammation and oxidative stress in lps-treated inflammation, resulting in an overall improvement in both macroscopic and histological parameters.

Journal: Journal of ethnopharmacology (2022)
Publication types: Journal Article
Sperm protein 17 targeting for epithelialovarian cancer treatment in the eraof modern immunoengineering.

Due to the vague symptomatology of the disease and a lack of effective screening methods, most patients with epithelial ovarian cancer (eoc) present late in their disease.Despite advances in chemotherapeutic agents, the prognosis of these patients has uniformly been extremely poor.Although cisplatin-based chemotherapy regimens induce responses in most of these patients, the patients invariably experience disease progression or relapses.In an attempt to improve the treatment outcome using a different therapeutic approach, immunotherapy was investigated nearly 20 years ago.Many tumor antigens that are potentially suitable for specific immunotherapy were identified, and many immunotherapeutic approaches were attempted.However, although some responses were observed, the results from clinical studies were generally disappointing.Recent advances in immunoengineering and successes observed among patients treated for refractory/relapsed hematologic malignancies have rekindled the interest to revisit specific cellular immunotherapy in eoc.In this review, we provide the rationale for immunotherapy of eoc, discuss the results of some of the historical studies on the use of cellular immunotherapy in eoc, outline the principles of modern immunoengineering that could be applied to treat the disease, and propose the re-evaluation of the cancer-testis antigen, sperm protein 17, for targeting by using modern immunoengineering technology.

Journal: Molecular therapy oncolytics (2021)
Publication types: Journal Article;Review
Human Umbilical Cord Blood Mononuclear Cells Ameliorate CCl4-Induced Acute Liver Injury in MiceviaInhibiting Inflammatory Responses and Upregulating Peripheral Interleukin-22.

Human umbilical cord blood mononuclear cells (h ucbmncs) show therapeutic effects on many inflammatory diseases.The deterioration of acute liver injury is attributed to excessive inflammatory responses triggered by damage-associated molecular patterns (damps) and pathogen-associated molecular patterns (pamps).Whether h ucbmncs treatment is a promising strategy for acute liver injury/failure needs to be investigated.methods:liver injury mice induced by pamps, damps, or damps plus pamps were developed.Damps included ccl4(carbon tetrachloride), apap (acetaminophen), and con a (concanavalin a).Pamps included klebsiella pneumoniae(k.p.) and salmonella typhimurium(s.Typhimurium).Damp plus pamp-induced liver injury was developed by sequential ccl4and k.p.administration.H ucbmncs were injected intravenously.results:h ucbmncs significantly prolonged mice survival time in damp plus pamp-induced liver failure but had no benefit in bacteria-infected mice.H ucbmncs significantly alleviated hepatic necrosis post ccl4/con a insult.In ccl4-induced acute liver injury, peripheral levels of interleukin (il)-22 were upregulated and liver regeneration was enhanced after treating with h ucbmncs at 48h.The levels of p62 and lc3b-ii, autophagy markers, were also upregulated in the h ucbmnc-treated group.conclusion:h ucbmncs as a kind of cell therapeutic strategy could attenuate acute liver injury in mice, which is executed by enhancing autophagy and regeneration in the liver via inhibiting inflammatory responses and upregulating peripheral il-22.

Journal: Frontiers in pharmacology (2022)
Publication types: Journal Article
Preparation, characterization of PLGA/chitosan nanoparticles as a delivery system for controlled release of DHA.

In this work, a novel dha-loaded nanoparticle with plga and chitosan (pcsdnp) was successfully prepared.The structure of pcsdnp and dha-loaded plga nanoparticles was measured by transmission electron microscope, scanning electron microscope, and differential scanning calorimeter.The interaction strength between dha, plga, and chitosan was evaluated through fourier transform infrared spectroscopy.The curves of controlled dha release and stabilities for different environmental factors of two nps were evaluated.Importantly, two nps were almost regularly spherical and the interactions were hydrogen bonds and electrostatic interactions between plga and chitosan.These nps had a good encapsulation rate (80.45%) and high-water solubility than the free dha molecule.In simulated gastrointestinal fluid, two nps showed a controlled-release pattern.Overall, pcsdnp had better stability and controlled-release effect with the synergy between cs and plga under the conditions of p h (2- 7), ionic strength (0- 500 m m), storage time (0- 42 d), and temperature (30- 80 °c).

Journal: International journal of biological macromolecules (2021)
Publication types: Journal Article
Association between oral health status and chronic obstructive pulmonary disease in Korean adults.

S this study aimed to elucidate the association between oral health status and chronic obstructive pulmonary disease (copd) in korean adults (≥ 40 years old) using a representative national dataset from the 6th korea national health and nutrition examination survey (6th knhanes, 2013-2015).Methods participants aged ≥ 40 years from the 6th knhanes who had received an oral and pulmonary function tests (n = 7719) were included in this study.The participant characteristics according to copd were compared using t-test and chi-squared test.Logistic regression analysis was used to estimate the association between oral health status and copd.Results participants with poor periodontal status exhibited a higher prevalence of copd.Moreover, patients with copd had a greater number of missing teeth than those without copd.The logistic regression model adjusted for demographic, socioeconomic, health- and oral health-related factors showed that the periodontal status was not significantly associated with copd, while participants with more missing teeth had a significantly increased possibility of having copd.Conclusions this study revealed that loss of teeth in adults aged ≥ 40 years was associated with copd.

Journal: International dental journal (2020)
Publication types: Journal Article
Hypobaric Unilateral Spinal Anaesthesia versus General Anaesthesia in Elderly Patients Undergoing Hip Fracture Surgical Repair: A Prospective Randomised Open Trial.

Intraoperative hypotension during hip fracture surgery is frequent in the elderly.No study has compared the haemodynamic effect of hypobaric unilateral spinal anaesthesia (husa) and standardised general anaesthesia (ga) in elderly patients undergoing hip fracture surgical repair.Methods we performed a prospective, randomised open study, including 40 patients aged over 75 years, comparing the haemodynamic effects of husa (5 mg isobaric bupivacaine with 5 μg sufentanil and 1 m l sterile water) and ga (induction with etomidate/remifentanil and maintenance with desflurane/remifentanil).An incidence of severe hypotension, defined by a decrease in systolic blood pressure of >40% from baseline, was the primary endpoint.Results the incidence of severe hypotension was lower in the husa group compared with that in the ga group (32% vs.71%, respectively, p=0.03).The median [iqr] ephedrine consumption was lower (p=0.001) in the husa group (6 mg, 0-17 mg) compared with that in the ga group (36 mg, 21-57 mg).Intraoperative muscle relaxation and patients' and surgeons' satisfaction were similar between groups.No difference was observed in 5-day complications or 30-day mortality.Conclusion this study shows that husa provides better haemodynamic stability than ga, with lower consumption of ephedrine and similar operating conditions.This new approach of spinal anaesthesia seems to be safe and effective in elderly patients undergoing hip fracture surgery.

Journal: Turkish journal of anaesthesiology and reanimation (2018)
Publication types: Journal Article
CISD1 Is a Breast Cancer Prognostic Biomarker Associated with Diabetes Mellitus.

Women with diabetes mellitus are believed to have increased risk of developing breast cancer and lower life expectancies.This study aims to depict the association between the cisd1, the co-expressed genes, and diabetes mellitus to offer potential therapeutic targets for further mechanical research.The tcga-brca rnaseq data is acquired.All the data and analyzed using r packages and web-based bioinformatics tools.Cisd1 gene expression was evaluated between tumor bulk and adjacent tissue.Immune cell infiltration evaluation was performed.Cisd1 expressed significantly higher in tumor tissue than that of the normal tissue, indicating poor overall survival rates.High expression level of cisd1 in tumor shows less p dc and nk cells penetration.There are 138 genes shared between cisd1 co-expressed gene pool in brca and diabetes mellitus related genes using "diabetes" as the term for text mining.These shared genes enrich in "cell cycle" and other pathways.Mcode analysis demonstrates that p53-independent g1/s dna damage checkpoint, p53-independent dna damage response, and ubiquitin mediated degradation of phosphorylated cdc25a are top-ranked than other terms.Cisd1 and co-expressed genes, especially shared ones with diabetes mellitus, can be the focused genes considered when addressing clinical problems in breast cancer with a diabetes mellitus background.

Journal: Biomolecules (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Ce(iv)-centered charge-neutral perovskite layers topochemically derived from anionic [CeTa2O7]-layers.

Layered perovskites have been extensively investigated in many research fields, such as electronics, catalysis, optics, energy, and magnetics, because of the fascinating chemical properties that are generated by the specific structural features of perovskite frameworks.Furthermore, the interlayers of these structures can be chemically modified through ion exchange to form nanosheets.To further expand the modification of layered perovskites, we have demonstrated an advance in the new structural concept of layered perovskite "charge-neutral perovskite layers" by manipulating the perovskite layer itself.A charge-neutral perovskite layer in [ce ivta2o7] was synthesized through a soft chemical oxidative reaction based on anionic [ce iiita2o7]-layers.The ce oxidation state for the charge-neutral [ce ivta2o7] layers was found to be tetravalent by x-ray absorption fine structure (xafs) analysis.The atomic arrangements were determined through scattering transmission electron microscopy and extended xafs (exafs) analysis.The framework structure was simulated through density functional theory (dft) calculations, the results of which were in good agreement with those of the exafs spectra quantitative analysis.The anionic [ce iiita2o7]-layers exhibited optical absorption in the near infrared (nir) region at approximately 1000 nm, whereas the level of nir absorption decreased in the [ce ivta2o7] charge-neutral layer due to the disappearance of the ce 4f electrons.In addition, the chemical reactivity of the charge-neutral [ce ivta2o7] layers was investigated by chemical reduction with ascorbic acid, resulting in the reduction of the [ce ivta2o7] layers to form anionic [ce iiita2o7]-layers.Furthermore, the anionic [ce iiita2o7]-layers exhibited redox activity which the ce in the perovskite unit can be electrochemically oxidized and reduced.The synthesis of the "charge-neutral" perovskite layer indicated that diverse features were generated by systematically tuning the electronic structure through the redox control of ce; such diverse features have not been found in conventional layered perovskites.This study could demonstrate the potential for developing innovative, unique functional materials with perovskite structures.

Journal: Chemical science (2021)
Publication types: Journal Article
Vancomycin Use in a Paediatric Intensive Care Unit of a Tertiary Care Hospital.

Vancomycin is one of the commonly used anti-microbial drugs in intensive care units (icus).Guidelines recommend maintaining therapeutic trough levels of vancomycin (10-20 mg/l).The success of achieving the recommended therapeutic concentration of vancomycin is influenced by several factors, and this is even more complex in children, particularly those admitted in the icu.Hence, we carried out the present study in children admitted in the icu who were administered vancomycin.Methods we carried out a chart review of children admitted in the paediatric icu unit of a tertiary care hospital over a period of 3 years.Information on their demographic factors, diagnoses, duration of hospital stay, vancomycin treatment (dose, frequency and time of administration) and concomitant drugs, and vancomycin trough levels were retrieved.Descriptive statistics were used for representing the demographic factors, and multivariable logistic regression analyses were carried out to assess the determining factors.Results one-hundred and two children were identified, of whom 13 had renal dysfunction.Two-hundred and fifty-two vancomycin trough levels were available, of which only 25% were observed in the recommended range (10-20 mg/l) amongst patients without any renal dysfunction and 22% amongst patients with renal dysfunction.Vancomycin was administered intravenously at an average [standard deviation (sd)] dose (mg/dose) of 13 (3.9) mostly either thrice or four times daily.Even in patients receiving vancomycin as a definitive therapy, only 40.9% achieved the recommended trough levels.Lower trough levels were associated with an increased risk of mortality.Nearly 4% of the levels were above 20 mg/l (toxic range).Seven children were suspected to have acute kidney injury (aki) during the course of therapy where the cumulative vancomycin dose and mortality rate was higher.Only one serum vancomycin level during augmented renal clearance was observed in the recommended range.All the patients received at least one concomitant drug that either had nephrotoxic potential or predominant renal elimination, and use of a greater number of such drugs was associated with an increased risk of aki.Conclusion the current vancomycin dosing strategy is ineffective in achieving therapeutic trough levels in children admitted to the icu.Sub-therapeutic vancomycin trough levels significantly increase the risk of mortality.

Journal: Paediatric drugs (2019)
Publication types: Journal Article
Inhibition of Glycogen Synthase Kinase-3: An Emerging Target in the Treatment of Traumatic Brain Injury.

Although traumatic brain injury (tbi) has been a major public health concern for decades, the pathophysiological mechanism of tbi is not clearly understood, and an effective medical treatment of tbi is not available at present.Of particular concern is sustained tbi, which has a strong tendency to take a deteriorating neurodegenerative course into chronic traumatic encephalopathy (cte) and dementia, including alzheimer's disease.Tauopathy and beta amyloid (aβ) plaques are known to be the key pathological markers of tbi, which contribute to the progressive deterioration associated with tbi such as cte and alzheimer's disease.The multiple lines of evidence strongly suggest that the inhibition of glycogen synthase kinase-3 (gsk-3) is a potential target in the treatment of tbi.Gsk-3 constitutively inhibits neuroprotective processes and promotes apoptosis.After tbi, gsk-3 is inhibited through the receptor tyrosine kinase (rtk) and canonical wnt signaling pathways as an innate neuroprotective mechanism against tbi.Gsk-3 inhibition via gsk-3 inhibitors and drugs activating rtk or wnt signaling is likely to reinforce the innate neuroprotective mechanism.Gsk-3 inhibition studies using rodent tbi models demonstrate that gsk-3 inhibition produces diverse neuroprotective actions such as reducing the size of the traumatic injury, tauopathy, aβ accumulation, and neuronal death, by releasing and activating neuroprotective substrates from gsk-3 inhibition.These effects are correlated with reduced tbi-induced behavioral and cognitive symptoms.Here, we review studies on the therapeutic effects of gsk-3 inhibition in tbi rodent models, and critically discuss the issues that these studies address.

Journal: Journal of neurotrauma (2016)
Publication types: Journal Article;Review
Risk factors for postoperative ileus after cesarean delivery.

Despite extensive data regarding risk factors for postoperative ileus in the general and colorectal surgery literature, few studies have identified risk factors specific to the obstetrical population.Objective this study aimed to identify factors associated with postoperative ileus following cesarean delivery.Study design this retrospective case-control study identified women who underwent cesarean delivery at a single hospital between january 2000 and january 2020 and subsequently developed postoperative ileus.Cases were matched in a 1:2 ratio with controls who underwent cesarean delivery and did not develop postoperative ileus.They were matched by age (±1 year) and body mass index (±1 kg/m2).Demographics, common comorbidities, obstetrical history, and delivery characteristics were analyzed.Results a total of 147 cases and 294 controls were identified.Cases and controls were similar in terms of parity, number of previous cesarean deliveries, labor preceding their cesarean delivery, incidence of chorioamnionitis, and presurgical diagnosis of hypothyroidism or chronic hypertension.Cases tended to have a diagnosis of preeclampsia (cases 23.1% vs controls 10.5%; p 1000 m l (cases 44.4% vs controls 11.6%; p 1000 m l, and exposure to general anesthesia were the remaining surgical factors associated with the development of ileus.These variables reflect both the complexity and most likely the duration of surgery that was required, although we note that we did not have operative time as a variable to explore.Preeclampsia was also identified as a comorbidity linked to the development of ileus.Conclusion a diagnosis of preeclampsia, exposure to general anesthesia, estimated blood loss >1 l, and transfusion of blood products were identified as potential risk factors for postcesarean ileus.

Journal: American journal of obstetrics & gynecology MFM (2022)
Publication types: Journal Article
Association of rare variants in genes of immune regulation with pediatric autoimmune CNS diseases.

There is a gap in the literature regarding genetic underpinnings of pediatric autoimmune cns diseases.This study explored rare gene variants implicated in immune dysregulation within these disorders.Methods this was a single-center observational study of children with inflammatory cns disorder who had genetic testing through next generation focused exome sequencing targeting 155 genes associated with innate or adaptive immunity.For in silico prediction of functional effects of single-nucleotide variants, polymorphism phenotyping v2, and sorting intolerant from tolerant were used, and combined annotation dependent depletion (cadd) scores were calculated.Identified genes were analyzed using kyoto encyclopedia of genes and genomes (kegg) pathway analysis.Results of 54 patients, 42 (77.8%) carried variant(s), among which 12 (22.2%) had 3-8 variants.Eighty-eight unique single-nucleotide variants of 55 genes were identified.The most variants were detected in unc13d, lrba, lyst, nod2, dock8, rnaseh2a, stat5b, and aire.The majority of variants (62, 70.4%) had cadd > 10.Kegg pathway analysis revealed seven genes associated with primary immunodeficiency (benjamini 1.40e - 06), six genes with nod-like receptor signaling (benjamini 4.10e - 04), five genes with inflammatory bowel disease (benjamini 9.80e - 03), and five genes with nf-kappa b signaling pathway (benjamini 1.90e - 02).Discussion we observed a high rate of identification of rare and low-frequency variants in immune regulatory genes in pediatric neuroinflammatory cns disorders.We identified 88 unique single-nucleotide variants of 55 genes with pathway analysis revealing an enrichment of nod2-receptor signaling, consistent with involvement of the pathway within other autoinflammatory conditions and warranting further investigation.

Journal: Journal of neurology (2022)
Publication types: Observational Study;Journal Article