syedzayyan/paper_mill_benchmark huggingface dataset:
Evaluation of the Antiasthmatic Activity of Carissa opaca in Animal Models

Carissa opaca stapf ex haines ( c.Opaca ) fruit is used traditionally in the treatment of respiratory illnesses including asthma.However, there is no scientific evidence supporting its antiasthmatic activity.The current study was conducted to evaluate its antiasthmatic effects using in vivo and in vitro approaches.The methanolic crude extract of c.Opaca fruit (co.cr.) was used and in vivo antiasthmatic activity was carried out using ovalbumin‐ (ova‐) sensitized and ova‐challenged balb/c mice.In in vitro bronchorelaxant activity of crude extract, aqueous and n‐hexane fractions of c.Opaca were carried out on isolated rat tracheal strips.Co.cr.(200 and 400 mg/kg) attenuated ovalbumin‐induced changes in lung histochemistry with % decrease in peribronchial inflammation of 14.1 ± 0.21 and 65.8 ± 0.22 and % decrease in total inflammatory cell count of 35.7 ± 2.80 and 53.3 ± 2.30 in bronchoalveolar lavage fluid.Co.cr., aqueous, and n‐hexane fraction of c.Opaca attenuated the precontractions induced by high k + (80 m m) and carbachol (1 μ m), respectively.In conclusion, the results showed that c.Opaca possesses antiasthmatic activity via relaxant effect on bronchial smooth muscle which is mediated through calcium channel blockade and antimuscarinic activity.This study provides scientific evidence of the traditional use of c.Opaca in the management of allergic asthma.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Evaluation of Hematological, Oxidative Stress, and Antioxidant Profile in Cattle Infected with Brucellosis in Southern Punjab, Pakistan

Brucellosis is a well‐known and harmful zoonotic disease that poses a severe threat to public health and wild and dairy animals.Due to a lack of monitoring and awareness, disease incidence has increased.Therefore, this study was conducted for the first time to ascertain the status of seroprevalence of brucellosis, hematological, oxidative stress, and antioxidant enzymes in different breeds of cattle reared under tropical‐desert conditions in pakistan.This study comprised 570 cattle of different breeds.We recorded some epidemiological traits, including age and gender.The blood samples were obtained from all the cattle, screened with rbpt, and then confirmed by elisa and pcr.The results recorded an overall 11.75%, 10.7%, and 9.64% prevalence of brucellosis based on rbpt, elisa, and pcr.We obtained nonsignificant results in different age and sex groups of cattle.The results showed significantly ( p ≤ 0.05) lower values of erythrocyte counts, hemoglobin quantity, hematocrit, lymphocytes, and monocytes in infected cases.The results showed that the total leukocyte and neutrophil cells significantly ( p ≤ 0.05) increased.The lipid peroxidation parameters (mda‐ and no‐scavenging activity of erythrocyte) increased significantly ( p ≤ 0.05) in infected cattle, whereas significantly reduced antioxidant enzymes like sod, rgsh, and cat were.Similarly, significantly lower serum albumin levels and total serum proteins were recorded in infected cattle.

Journal: BioMed Research International (2022)
Publication types: Research Article;
ERK1/2 Pathway Is Involved in the Enhancement of Fatty Acids from Phaeodactylum tricornutum Extract (PTE) on Hair Follicle Cell Proliferation

Extractions from phaeodactylum tricornutum have been widely studied and evaluated to various biological effects.The aim of this study was to investigate the promotional effect of p.Tricornutum extract (pte) on the erk1/2 signaling pathway involved in hair follicle cell proliferation.In order to illuminate the enhancement of pte on hair growth by promoting proliferation of hair follicle cells, the activities of human hair follicle outer root sheath cell (hforsc), human hair follicle germinal matrix cells (hfgmc), and hair epithelial melanocytes (hem) were observed under pet treatment.Levels of keratins, pkc ζ , erk1/2, and p38 mapk in hair follicle cells were determined by western blotting to illustrate the mechanisms of pte effects on hair growth.Analyzed by gc‐ms, the main polyunsaturated fatty acids which were 9.43% of total fatty acids in pte were linolenic acid, linoleic acid, eicosapentaenoic acid, and docosahexaenoic acid.Melanin content and tyrosinase activity in hem were measured.The results showed that pte exhibited remarkable enhancement on cell proliferation.Melanin production was inhibited by pte treatment, while keratin‐14, keratin‐15, and keratin‐17 levels on hair follicle cells were elevated at different concentrations.The promotions of erk1/2 and p38 mapk levels indicated that the erk1/2 signaling pathway is involved in the proliferation of hair follicle cells.These results are the evidence that pte potentially deserves further study as a new natural candidate for hair care applications.

Journal: BioMed Research International (2020)
Publication types: Research Article;
Efficiency of Nanohydroxyapatite on Repairing Type II Diabetes Dental Implant-Bone Defect

The purpose of this study was to see how a nanohydroxyapatite (n‐ha) composite polyamide 66 (pa66) affected the repair of bone defects in diabetics with titanium implants, as well as to develop experimental materials for the creation of the interface between bone tissue and titanium implants.Rabbit bone marrow mesenchymal stem cells (mscs) were isolated using n‐ha/pa66 composite material, and the effect of coculture with the material on cell proliferation was analyzed after induction of mineralization.Bone defect models of diabetic experimental rabbits and titanium implants were prepared.Normal rabbits with bone defects were used as control (nc group, n = 8).After the diabetic bone defect (dm group, n = 8) and the implantation of n‐ha/pa66 composite material (n‐ha/pa66 group, n = 8), the differences in body weight, blood glucose, scanning electron microscopy of the implant‐bone interface, bone mineral density, new bone trabecular parameters, histomorphology, and biomechanical properties of the implant‐bone interface were compared and analyzed.In vitro test results showed that msc cell growth could be promoted by mineralization induction, the cell growth condition was good after coculture with n‐ha/pa66, and the proliferation activity of mscs was not affected by the material.In vivo test results showed that the body weight of the dm group and n‐ha/pa66 group was considerably inferior to that of the nc group, and the blood glucose was dramatically superior to that of the nc group ( p &lt; 0.05).However, the body weight of the n‐ha/pa66 group was dramatically superior to that of the dm group ( p &lt; 0.05).The bone mineral density, bone volume fraction (bv/tv), bone surface area fraction (bs/bv), bone trabecular thickness (tb.th), bone trabecular number (tb.n), bone trabecular area, and biomechanical properties in the dm group were considerably inferior to those in the nc group and n‐ha/pa66 group ( p &lt; 0.05).The trabecular space (tb.sp) in the nc group and n‐ha/pa66 group was dramatically superior to that in the nc group ( p &lt; 0.05).The bone mineral density, bv/tv, bs/bv, tb.th, tb.n, trabecular area, and biomechanical properties of the n‐ha/pa66 group were dramatically superior to those of the nc group ( p &lt; 0.05), while tb.sp was considerably inferior to that of the nc group ( p &lt; 0.05).These findings showed that the n‐ha/pa66 material had good biocompatibility and minimal cytotoxicity, and that filling the space between the surrounding bone and the titanium implant can enhance bone repair.This research paved the way for future research into the tissue‐engineered bone in the field of oral surgery.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Efficacy of Mesotympanum Injection and Posterior Auricular Injection in Sudden Hearing Loss of Diabetes Patients

The efficacy of tympanum injection and posterior auricular injection in diabetes with sudden hearing loss (shl) was analyzed.A mobile terminal‐based portable pure tone audiometry system and its processing method were established.Based on mobile terminals, a portable pure tone audiometry system including an android system, pure sound signal generation, pure tone hearing threshold, and client module was established.A masking model and self‐adaptive algorithm were used to detect and reduce noise.Besides, the performance of the portable pure tone audiometry system was detected.A total of 46 diabetes patients with shl diagnosed at the otolaryngology department in bei chen hospital between august 2019 and november 2021 were selected as the research objects and randomly divided into the retroauricular group (posterior auricular injection) and the tympanic group (tympanum injection).Each group included 23 cases.All patients received pure tone audiometry (pta) before and after the treatment.The changes in fasting blood glucose (fpg), 2h postprandial blood glucose (2h pg), and glycosylated hemoglobin (hb a1c) of the patients were monitored before and after the treatment.Besides, tinnitus loudness visual analog scale (vas), pain vas, efficacy, and the incidence of adverse reactions of the patients in two groups were compared.The results indicated that the hear threshold error detected by the medical audiometer and a portable pure tone audiometry system was within 2d b.Before the optimization, there was an error of about 10d b between the hear thresholds detected by the self‐adaptive algorithm and a medical audiometer.After the treatment, the hear threshold and average pta of the patients in the retroauricular and the tympanic groups under different frequencies were both reduced compared with those before the treatment ( p &lt; 0.05).The tinnitus vas score in the retroauricular group was decreased more notably than that in tympanic group ( p &lt; 0.01), and the pain vas score was much lower than that in the tympanic group ( p &lt; 0.001).The comparison of fpg, 2h pg, hb a1c, the proportions of cured, significantly effective, effective, ineffective patients, and the total effective rate in the patients in the retroauricular and the tympanic groups before and after the treatment all showed no statistical differences ( p &gt; 0.05).The incidence of adverse reactions in tympanic group after the treatment was dramatically higher than that in retroauricular group ( p &lt; 0.001).The above results demonstrated that posterior auricular injection showed potential application values in the treatment of shl with diabetes and established a portable pure tone audiometry system as well as its noise processing method.

Journal: BioMed Research International (2022)
Publication types: Clinical Study;
Efficacy and Safety of rCCK96-104PE38 Targeted Drug in the General Surgical Treatment of Colon Cancer

To evaluate the clinical efficacy and safety of the r cck 96-104 pe38 targeted drug in patients with colon cancer in general surgery, data of 80 patients with colon cancer who were admitted to the hospital from april 2019 to july 2021 were selected and randomly divided into the treatment group and the control group, with 40 cases in each group.Patients in the treatment group were treated with the r cck 96-104 pe38 targeted drug, and those in the control group were treated with oxaliplatin.The treatment efficiency and incidence of adverse reactions were compared between the two groups.The inverse cholecystokinin (cck 96-104 ) was fused with pseudomonas aeruginosa exotoxin (pe38 toxin) through the gene amplification technique to construct a prokaryotic expression vector.Then, the r cck 96-104 pe38 was purified by ni‐nitrilotriacetate (ni‐nta) affinity chromatography, and the antitumor activity of r cck 96-104 pe38 was verified.The results showed that the amplified r cck 96-104 pe38 sequence was correct and the p et‐28a prokaryotic expression system was adopted to successfully achieve active expression.The purified recombinant protein could induce the apoptosis of colon cancer cells in vitro and inhibit tumor growth in vivo.The total effective rate in the treatment group (80%, 32/40) was higher than that in the control group (60%, 24/40) ( p &lt; 0.05).To sum up, the recombinant toxin r cck 96-104 pe38 could not only specifically adsorb the colon cancer cells with high expression of cck2r but also effectively inhibit tumor tissue growth and proliferation.Besides, the r cck 96-104 pe38 protein had a good anticancer effect that helped effectively reduce the incidence of adverse reactions in patients, which was worthy of promoting.

Journal: BioMed Research International (2022)
Publication types: Clinical Study;
Effects of Ulinastatin on Proliferation and Apoptosis of Breast Cancer Cells by Inhibiting the ERK Signaling Pathway

To explore the effects of ulinastatin on the proliferation and apoptosis of breast cancer cells and the relevant mechanism of action.Methods .Breast cancer cells (mcf‐7) were cultured and randomly divided into three groups, namely, control group, ulinastatin group, and ulinastatin+extracellular‐regulated protein kinase (erk) inhibitor group.Then, the cell counting kit‐8 (cck‐8) assay was carried out to detect the effect of ulinastatin on the viability of breast cancer cells.The effects of ulinastatin on the proliferation and apoptosis of breast cancer cells were determined via ed u staining and hoechst 33258 staining assays, respectively.The messenger ribonucleic acid (m rna) and protein expression levels of erk and forkhead box o3 (foxo3) in breast cancer cells were measured through reverse transcription‐polymerase chain reaction (rt‐pcr) and western blotting.Results .In comparison with the control group, the ulinastatin group displayed decreased viability of breast cancer cells, a decreased positive rate of 5‐ethynyl‐2 ′ ‐deoxyuridine (ed u) staining, an increased positive rate of hoechst 33258 staining, and reduced m rna and protein levels of erk and foxo3 in breast cancer cells.Compared with those in the ulinastatin group, the viability of breast cancer cells was lowered, the positive rate of ed u staining was reduced, the positive rate of hoechst 33258 staining was raised, and the m rna and protein levels of erk and foxo3 in breast cancer cells clearly declined in the ulinastatin+erk inhibitor group.Conclusion .Ulinastatin inhibits the proliferation and promotes the apoptosis of breast cancer cells.The possible mechanism of action is associated with the suppression of the erk signaling pathway.

Journal: BioMed Research International (2021)
Publication types: Research Article;
HPV-16 Expression and Loss of Cell Differentiation in Primary Bladder Tumors

Primary bladder tumors have a high degree of malignancy.To investigate the expression of human papillomavirus type 16 (hpv‐16) in primary bladder tumors and the loss of cell differentiation and to explore the significance of hpv‐16 detection, it is expected to be a disease.Treatment provides a theoretical basis.Methods.Fifty‐seven patients with primary bladder tumors admitted to our hospital from january 2019 to january 2022 were selected as the research subjects, and they were divided into hpv‐related groups according to the human papillomavirus (hpv) infection status ( n = 28) and hpv unrelated group ( n = 29).The general data of patients were collected, the expression of hpv‐16 in bladder tissue samples was detected, and the correlation between pathological parameters and hpv‐16 expression was analyzed.Results.Among hpv subtypes, hpv 16 subtype accounted for the highest proportion, followed by hpv‐18 and hpv‐6 subtypes; there was no significant difference in tumor stage (stage 1, stage a, stage 2a) between the hpv‐related group and the hpv‐unrelated group (stage 1, stage a, and stage 2a).P &gt; 0.05); there was no significant difference in postoperative pathological expression (high expression and low expression) of patients ( p &gt; 0.05); there was no statistical difference in age and gender between hpv‐related and hpv‐unrelated groups ( p &gt; 0.05), hpv‐related group and hpv‐unrelated group compared daily regular drinking and smoking status, the difference was statistically significant ( p &lt; 0.05); hpv‐16 expression was not correlated with tumor differentiation degree and age of patients ( p &gt; 0.05); the area under the curve (auc) of hpv‐16 for judging primary bladder tumor expression and cellular molecular deletion was 0.891, with a sensitivity of 83.94% and a specificity of 88.57%.Conclusion.Hpv‐16 is an upper, expressed in primary bladder tumors and will participate in the differentiation and loss of cells, which can provide effective guidance and basis for the diagnosis of primary bladder tumors, which is an important factor for judging the pathological stage and prognosis of patients and can provide a theoretical reference for the formulation of therapeutic measures.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Groundwater Suitability Evaluation Using Entropy Weightage Quality Index (EWQI) Model and Human Health Cancer Risk Assessment of Heavy Metal in Eastern India

This study evaluated the groundwater using the entropy weightage quality index model (ewqi).Eighteen samples were taken from the different wellbores during premonsoon seasons in 2021.The present study is aimed at developing a comprehensive approach for groundwater quality assessment and associated health risk along with the cancer risk due to the presence of heavy metals.The water quality of ranchi city was found to be better except in the western zone.Principal component analysis (pca) revealed that arsenic (as) was the most influencing element that deteriorated the potability of water which supports our study.The study looked at cancer and noncancer health hazards connected with heavy metal music.The value of hazardous quotient (hq) was observed to be relatively higher in as (hq &gt; 1) and ni, followed by mn &gt; fe &gt; zn &gt; cu.Also, the children were at higher risk than adults.The cancer risk associated with arsenic was investigated and found that the northern part and southeast‐west (lapung block) of the study are at higher risk.Prolonged ingestion of as causes diseases like arsenicosis that leads to enhanced chances of cancer risk.This research provides an immense research database to assess the potability of drinking water in a similar city like ranchi.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Heterogeneous Network-Based Inductive Matrix Methods for Predicting Biomedical Gene Disease

Prediction of gene‐disease associations has grown in popularity in recent biomedical research.However, positive and unlabeled (pu) issues and limited gene‐disease association data are common concerns with present association prediction algorithms.A gene‐disease association prediction approach based on katz‐enhanced inductive matrix completion is suggested in light of the abovementioned flaws.Preestimate based on the katz technique and refined estimation based on the inductive matrix completion approach makes the model.The katz technique is utilized to preestimate the gene‐disease association on the basis of gene‐disease heterogeneous network to mitigate the effects of association data‐sparse and pu issues.The katz technique, however, necessarily introduces some noise when predicting gene‐disease connections due to the similarity network’s quality limitations.Therefore, the elastic net regularization approach is utilized to increase the resilience of the conventional inductive matrix completion model.As a result, the prediction effect of gene‐disease connections is increased using robustness and a better inductive matrix completion model.The experimental findings demonstrate that the proposed model has dramatically increased recall and precision compared to widely used gene‐disease association prediction approaches.It can also resolve the typical cold‐start issue in association prediction.The proposed kimc method may consider integrating more diverse biological data sources in the future and also aid in the effective extraction of the feature data of genes and diseases with higher correlation from this biological data to improve the prediction effect.

Journal: BioMed Research International (2023)
Publication types: Research Article;
Effects of Sacubitril/Valsartan on the Expression of CaMKII/Cav/sub1.2 in Atrial Fibrillation Stimulation Rabbit Model

And objective .Atrial fibrillation (af) is linked to high morbidity and death rates throughout the world due to limited therapeutic options and thus presents a major challenge to the developed and developing countries.In this study, we aim to investigate the influence of sacubitril/valsartan (sac/val) treatment on the calmodulin‐dependent protein kinase ii (ca mkii)/ca v 1.2 expression in af models.Methods .Overall, 18 rabbits were randomly divided into control, pacing (600 beats/min), and pacing+sac/val groups.The rabbits in the pacing+sac/val cohort received oral sac/val (10 mg/kg twice daily) across the 21‐day investigation period.After three weeks, the atrial effective refractory period (aerp) and af induction rate were compared.Hl‐1 cultures were exposed to fast pacing (24 h) with and without lbq657 (active sacubitril form)/valsartan.Western blots were used for detecting ca v 1.2 and ca mkii expression within atrial muscles of the rabbits and hl‐1 cultures of af model.Results .In comparison to the sham cohort, the af induction rate was markedly increased together with aerp reduction within pacing cohort.Such changes were markedly rescued through sac/val treatment in pacing+sac/val cohort.The proteomic expression profiles of ca mkii and ca v 1.2 showed that the ca mkii expression was markedly upregulated, while ca v 1.2 expression was downregulated in the pacing cohort.Importantly, these effects were absent in pacing+sac/val cohort.Conclusion .Results of this study show that sac/val treatment regulates the expression of ca mkii/ca v 1.2 and could alter this pathway in atrial rapid electrical stimulation models.Therefore, this investigation could contribute to a novel strategy in af therapeutics in clinical settings.

Journal: BioMed Research International (2022)
Publication types: Research Article;