syedzayyan/paper_mill_benchmark huggingface dataset:
High Expression of PDE8B and DUOX2 Associated with Ability of Metastasis in Thyroid Carcinoma

Hormone is an independent factor that induces differentiation of thyroid cancer (tc) cells.The thyroid-stimulating hormone (tsh) could promote the progression and invasion in tc cells.However, few genes related to hormone changes are studied in poorly differentiated metastatic tc.This study is aimed at constructing a gene set’s coexpression correlation network and verifying the changes of some hub genes involved in regulating hormone levels.Methods.Microarray datasets of tc samples were obtained from public gene expression omnibus (geo) databases.R software and bioinformatics packages were utilized to identify the differentially expressed genes (degs), important gene module eigengenes, and hub genes.Subsequently, the gene ontology (go) enrichment analysis was constructed to explore important biological processes that are associated with the mechanism of poorly differentiated tc.Finally, some hub gene expressions were validated through real-time pcr and immunoblotting.Results.Gene chip with category number gse76039 was analyzed, and 1190 degs were screened with criteria of p &lt; 0.05 and ∣ log 2 foldchange ∣ &gt; 2 .Our analysis showed that human dual oxidase 2 (duox2) and phosphodiesterase 8b (pde8b) are the two important hub genes in a coexpression network.In addition, the validated experimental results showed that the expression levels of both duox2 and pde8b were elevated in poorly differentiated metastatic tc tissues.Conclusion.This study identified and validated that duox2 and pde8b were significantly associated with the metastasis ability of thyroid carcinoma.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Research Article;
GNAI2 Is a Risk Factor for Gastric Cancer: Study of Tumor Microenvironment (TME) and Establishment of Immune Risk Score (IRS)

Although the g protein subunit α i2 (gnai2) is upregulated in multiple cancers, its prognostic value and exact role in the development of gastric cancer (gc) remain largely unknown.Methods.This study evaluated the effect of gnai2 on the tumor microenvironment (tme) in gc, constructed an immune risk score (irs) model based on differentially-expressed immune genes, and systematically correlated gnai2 and epigenetic factor expression patterns with tme and irs.Also, rt-q pcr, flow cytometry, western blotting (wb), and transwell assays were carried out to explore the regulatory mechanism of gnai2 in gc.Results.High gnai2 expression was associated with poor prognosis.Cytokine activation, an increase in tumor-infiltrating immune cells (tiic), and the accumulation of regulatory t cells in the tumor immune cycle were all promoted by the tme, which was significantly associated with gnai2 expression.Two different differentially expressed m rna (der) modification patterns were determined.These two ders-clusters had significantly different tme cell infiltrations and were classified as either noninflamed or immune-inflamed phenotypes.The irs model constructed using differentially expressed genes (degs) had great potential in predicting gc prognosis.The irs model was also used in assessing clinicopathological features, such as microsatellite instability (msi) status, epithelial-mesenchymal transition (emt) status, clinical stages, tumor mutational burden (tmb), and tumor immune dysfunction and exclusion (tide) scores.Low irs scores were associated with high immune checkpoint gene expression.Cell and animal studies confirmed that gnai2 activated pi3k/akt pathway and promoted the growth and migration of gc cells.Conclusion.The irs model can be used for survival prediction and gnai2 serves as a candidate therapeutic target for gc patients.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Functions of Heparin Sodium Injection in the Prevention of Peripherally Inserted Central Catheter-Related Venous Thrombosis in NSCLC Patients during Postoperative Chemotherapy

This study intended to analyze hazardous factors of venous thrombosis by comparing the effect of different doses of heparin sodium injection on the incidence rate of peripherally inserted central catheter (picc)-related venous thrombosis in non-small cell lung carcinoma (nsclc) patients during postoperative chemotherapy.Methods.425 nsclc patients who received picc catheterization in cancer hospital chinese academy of medical sciences, shenzhen hospital from july 2019 to july 2021 were collected.Based on their different pathological types, patients were given two different chemotherapy regimens: pemetrexed+cisplatin or paclitaxel+cisplatin.Patients were grouped according to the different doses of heparin sodium injection adopted.Control group ( n = 140 ).Catheters were sealed with 10 m l saline only.Group i ( n = 142 ).In addition to routine maintenance with normal saline, 2 m l of 10 iu/m l heparin sodium injection was sealed in the catheters under positive pressure every time after catheterization.Group ii ( n = 143 ).In addition to routine maintenance with normal saline, 5 m l of 10 iu/m l heparin sodium injection was sealed in the same manner as group i.The baseline characteristics of the three groups of patients were compared by statistical means.Doppler ultrasonography was applied to check the venous thrombosis.The hazardous factors of venous thrombosis were analyzed through correlation analysis and binary logistic regression method.Results.The incidence rates of thrombosis in the control group, group i, and group ii were 20.00%, 7.04%, and 2.09%, respectively, with statistically significant differences ( p &lt; 0.01 ).Additionally, through the collinear correlation analysis of baseline characteristics, a significant correlation between the dosage of heparin sodium injection and the incidence of thrombosis was observed ( p &lt; 0.05 ), but there were no significant differences between other baseline data and the incidence of thrombosis ( p &gt; 0.05 ).Binary logistic regression analysis revealed that postoperative use of heparin sodium injection (group i: or = 0.312 ; p = 0.003 ; group ii: or = 0.082 , p &lt; 0.001 ) was a protective factor for preventing thrombosis.In addition, the thromboprophylaxis effect of group ii was better than that of group i.No serious adverse reactions were found in safety analysis.Conclusion.Heparin sodium could significantly lower the incidence rate of picc-related venous thrombosis in nsclc patients during postoperative chemotherapy.Heparin sodium injection is safe enough to be promoted among picc patients with a high risk of venous thrombosis.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
FTX Regulated miR-153-3p/FOXR2 to Promote Cisplatin Resistance in Ovarian Cancer

The present study was aimed at exploring the role of ftx in cisplatin (ddp) resistance in ovarian cancer (oc).Methods.Qpcr was applied to evaluate m rna expression in oc tissue and cells.Cck-8 assay was conducted to evaluate cell proliferation.Transwell chamber assay was performed to evaluate invasion of skov3/ddp cells.The protein expression was evaluated via western blot assay.Flow cytometry was performed to evaluate the apoptosis of skov3/ddp cells.Results.The expression of ftx in ddp-resistant cells was observably higher in contrast to ddp-sensitive cells and normal ovarian cells.Ftx was higher expressed in ddp-resistant tissues by comparison with ddp-sensitive tissues.Knockdown of ftx obviously suppressed the proliferation ability invasion ability of skov3/ddp cells.Knockdown of ftx obviously enhanced apoptosis of skov3/ddp cells.Mi r-153-3p was proved to be directly regulated by ftx via the luciferase reporter assays.By comparison with normal cells, mi r-153-3p was lower expressed in oc cells.Mi r-153-3p was lower expressed in skov3/ddp cells in contrast to skov3 cells.More interestingly, ftx reversed the inhibiting influence of mi r-153-3p on cisplatin resistance of oc cells.Moreover, mi r-153-3p was proved to directly regulate foxr2.Knockdown of mi r-153-3p attenuated the inhibitory influence of knockdown foxr2 on cisplatin resistance of oc cells.Conclusion.Ftx regulated mi r-153-3p/foxr2 to promote cisplatin resistance via inhibiting the apoptosis and promoting the viability and invasion in oc.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
Feasibility of Constructing an Automatic Meniscus Injury Detection Model Based on Dual-Mode Magnetic Resonance Imaging (MRI) Radiomics of the Knee Joint

To explore the feasibility of automatically detecting the degree of meniscus injury by radiomics fusion of dual-mode magnetic resonance imaging (mri) features of sagittal and coronal planes of the knee joint.Methods.This retrospective study included 164 arthroscopically confirmed meniscus injuries in 152 patients admitted to the department of orthopaedics of our hospital from july 2018 to march 2021.A total of 1316-dimensional radiomics signatures were extracted from single-mode sagittal and coronal plane images of menisci, respectively.Then, the sagittal and coronal plane features were fused to form a dual-mode joint feature group with a total of 2632-dimensional radiomics signatures.The minimum redundancy maximum relevance (m rmr) algorithm and the least absolute shrinkage and selection operator (lasso) regression were used to select features and generate optimal radiomics signatures.The single-mode sagittal plane feature model (model 1), single-mode coronal plane feature model (model 2), and the combined sagittal and coronal plane feature model (model 3) performance were tested by receiver operating characteristic (roc) curves and delong test.The calibration curve test was used to verify the reliability of radiomics signatures of the three models.Results.The average intra- and interobserver intraclass correlation coefficients (iccs) of the most significant 8-dimensional radiomics signatures of model 1 and model 2 were 0.935 (range 0.832-0.998) and 0.928 (range 0.845-0.998), respectively.All the three models had good detection performance; model 3 had the most significant performance (the areas under the curve (aucs) of training, and validation sets were 0.947 and 0.923, respectively), which was superior to model 1 (aucs of training set and validation set were 0.889 and 0.876, respectively) and model 2 (aucs of training set and validation set were 0.831 and 0.851, respectively).The detection probability of training and validation sets in the three models was highly consistent with the actual clinical probability.Conclusions.It is feasible to establish a model for automatic detection of meniscus damage by means of radiomics.The detection performance of the dual-mode knee mri model is better than that of any single-mode model, showing potent feature analysis ability and outstanding detection performance.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
Expression and Clinical Trends of Pct Combined with Lactate Clearance in Sepsis

To study the expression and clinical trend of procalcitonin (pct) + lactate clearance in sepsis.Methods.A total of 63 septic shock patients enrolled in our hospital from march 2018 to march 2021 were recruited as group a, and the 87 septic patients admitted during the same period were recruited as group b.The pct, lactic acid, and lactate clearance were compared between the two groups.Results.The pct and lactic acid in group a were higher than those in group b at the time of diagnosis, 1 d, 2 d, and 3 d after treatment, but the lactate clearance rate at 1 d, 2 d, and 3 d after treatment was lower than that in group b ( p &lt; 0.05 ).The pct and lactate at the time of diagnosis, 1 d, 2 d, and 3 d after treatment in the good prognosis group were lower than those in the poor prognosis group, but the lactate clearance rate at 1 d, 2 d, and 3 d after treatment was higher than that in the poor prognosis group ( p &lt; 0.05 ).Disease severity and prognosis were positively correlated with pct and lactate, but negatively correlated with lactate clearance rate ( p &lt; 0.05 ).Conclusions.Pct and lactate clearance rate might serve as accurate predictors of the severity of sepsis and prognosis.It merits promotion in clinical setting.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;
Exploration of the Application Effect of the Darongtong Course Model Based on Deep Learning Enhancement in Nursing

In order to explore the application effect of the darongtong course model based on deep learning enhancement in nursing, a total of 500 students in the school are investigated.The students in the contrast set are given the traditional teaching mode, and the students in the research set are provided with a teaching method based on deep learning‐enhanced darongtong mode.The evaluation results, teaching quality, students' cognition of teaching effect and their satisfaction with teaching mode before and after teaching were compared between the two groups.From the experimental results, it can be seen that the application of datong general teaching based on deep learning in school nursing students can not only deepen students' understanding of theoretical knowledge, improve clinical skills, cultivate students' nurse patient communication skills and clinical thinking, but also improve students' subjective initiative.It is clearly evident that the darongtong course model based on deep learning enhancement in nursing can stimulate students' interest in learning, and improve their self‐study ability and problem‐solving ability effectively.

Journal: Contrast Media & Molecular Imaging (2022)
Publication types: Research Article;
Experimental Research on the Antitumor Effect of Human Gastric Cancer Cells Transplanted in Nude Mice Based on Deep Learning Combined with Spleen-Invigorating Chinese Medicine

Gastric cancer is still the fifth most common malignant tumor in the world and has the fourth highest mortality rate in the world.Gastric cancer is difficult to treat because of its unobvious onset, low resection rate, and rapid deterioration.Therefore, humans have been working hard to combat gastric cancer.At present, the most commonly used treatment method is radiotherapy.However, this method will damage the normal tissues of the irradiated area while treating malignant tumor cells.It not only has side effects of damage to the patient’s skin and mucous membranes but also needs high-rate radiotherapy and has high cost for chemotherapy.In order to solve these problems, it is necessary to find new treatment methods.This article proposes the use of chinese medicine to invigorate the spleen to inhibit human gastric cancer cells.This article combines modern machine learning technology with traditional chinese medicine and combines traditional chinese medicine physiotherapy with western medicine nude mouse transplantation experiments.The treatment of tumors in chinese medicine is based on the theory of chinese medicine and has different characteristics.Western medicine has the advantage of permanently injuring patients.The process of the experiment is to transplant human-derived gastric cancer cells into nude mice.After grouping treatments and obtaining comparative data, deep learning techniques are used to analyze the properties of chinese medicines for strengthening the spleen and to compare the properties of chinese medicines for strengthening the spleen.The experimental results showed that the tumor inhibition rate of mice using fluorouracil was 18%, the tumor inhibition rate of mice using low-dose chinese medicine was 16%, and the tumor inhibition rate of mice using high-dose chinese medicine reached 52%.80 days after the experiment, the survival rate of mice using high-dose chinese medicine is 100% higher than that of mice without treatment.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
Examination on Risk Factors of Infertility Caused by EMT and Their Correlation with VEGF, TNF-α, IL-6, IL-10, and IL-17

In order to explore the risk factors of infertility caused by endometriosis (emt) and their correlation with vascular endothelial growth factor (vegf), tnf‐ α , il‐6, il‐10, and il‐17, endometriosis sufferers admitted to our hospital from january 2021 to may 2022 are selected to conduct the examination.According to the pregnancy of patients, patients were included in the simple emt set and emt combined infertility set, with 50 cases in each group.The degree of dysmenorrhea is evaluated by the vas score, and luminex liquid protein is used to analyze the standards of the tumor necrosis factor (tnf‐a), interleukin (il)‐10, il‐6, il‐17, and vegf.Logistic multifactor regression decomposition is applied to analyze the risk factors of infertility in emt sufferers.Besides, the standards of vegf, tnf‐ α , il‐6, il‐10, and il‐17 in sufferers with different periods/agony degrees are evaluated, and the correlation of different periods/agony degrees with vegf, tnf‐ α , il‐6, il‐10, and il‐17 is analyzed.The results show that the different r‐afs periods are notoriously positively correlated with vegf, tnf‐ α , il‐6, il‐10, and il‐17 (all p &lt; 0.05), and the vas score is notoriously positively correlated with the abovementioned factors.

Journal: Contrast Media & Molecular Imaging (2022)
Publication types: Research Article;
Efficacy, Safety, and Prognosis of Sequential Therapy with Tamoxifen and Letrozole versus Letrozole Monotherapy for Breast Carcinoma

To explore the efficacy, safety, and patient prognosis of letrozole (ltz) alone or in sequence with tamoxifen (tam) for the treatment of breast carcinoma (bc).Methods.In this retrospective study, 150 patients with bc who received treatment in the first people’s hospital of ningyang county between january 2012 and january 2017 were selected.According to different treatment methods, 99 cases receiving sequential therapy with tam and ltz were included in the research group, and the remaining 51 patients receiving ltz monotherapy were selected as the control group.The efficacy, safety, survival rate, recurrence rate, and blood lipid indices (total cholesterol, tc; triglyceride, tg; high-density lipoprotein cholesterol, hdl-c; and low-density lipoprotein cholesterol, ldl-c) of the two groups were observed and compared.Results.The overall response rate of the research group was statistically higher than that of the control group, and the incidence of adverse reactions was significantly lower.No evident difference was observed in 1-, 3-, or 5-year survival rates between the two groups, while the 3-5-year recurrence rate was obviously lower, and the improvement of blood lipid indices was significantly better in the research group compared with the control group.Conclusion.Ltz alone or in sequence with tam is effective and safe for the treatment of bc, which can significantly improve the prognosis and blood lipid indices of bc patients.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
Efficacy of Fenestration Decompression for the Treatment of Oral and Maxillofacial Cysts

Surgery is the mainstay of treatment for oral and maxillofacial cysts.Compared with open surgery that will bring more harm to patients, fenestration decompression, as a surgical method with good curative effects and little damage, has received increasing attention in the treatment of oral and maxillofacial cysts.Methods.The clinical data of 135 patients with oral and maxillofacial cysts visited the fifth central hospital of tianjin between june 2019 and september 2021 were collected for retrospective analysis.Patients were assigned to two groups based on the treatment plan implemented: the control group ( n = 64 ) treated with curettage of cysts and the observation group ( n = 71 ) with fenestration decompression.Therapeutic efficacy parameters and surgical indicators were detected.Additionally, postoperative cyst, pain, complication rate, and recurrence, as well as life quality six months after treatment, were evaluated and compared.Results.In comparison with the control group, the observation group was observed to have a higher total effective rate, less operation time, shorter hospital stays, and less intraoperative bleeding ( p &lt; 0.05 ).In addition, the shrinkage rate, shrinkage volume, and postoperative density of the cyst cavity were higher in the observation group than in the control group ( p &lt; 0.05 ).The observation group also outperformed the control group with lower postoperative vas score, complication rate, and half-year recurrence rate ( p &lt; 0.05 ).Furthermore, significantly better life quality was determined in the observation group after half a year of treatment ( p &lt; 0.05 ).Conclusion.Fenestration decompression is highly effective in treating oral and maxillofacial cysts, contributing to fewer complications, markedly relieved symptoms, shorter hospitalization time, well-preserved facial nerves, and low recurrence rate in the later period, which is worthy of clinical promotion.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
Efficacy Analysis of DSA-Guided Bronchial Arterial Chemoembolization Interventional Therapy in Patients with Middle-Advanced Primary Bronchial Lung Cancer

To investigate the clinical efficacy of digital subtraction angiography- (dsa-) guided bronchial arterial chemoembolization (bace) in patients with primary bronchial lung cancer.Methods.A total of 178 patients with primary intermediate and advanced bronchial lung cancer admitted to our hospital from february 2019 to march 2020 were selected as the subjects, and they were divided into control group (84 cases) and observation group (94 cases) according to the different chemotherapy regimens adopted by the patients.The control group was treated with traditional perfusion chemotherapy, and the observation group was treated with dsa-guided bace interventional therapy, treated for 4 cycles, and followed up until the end of june 2021.The short-term clinical efficacy, hemoptysis remission, and incidence of adverse reactions were compared between the two groups.The mortality and recurrence rates between the two groups from treatment to the end of follow-up were counted, and the quality of life after treatment and 1 year after treatment were compared.Results.The short-term remission rate (73.40% vs.58.33%), disease control rate (93.62% vs.84.52%), hemoptysis remission rate (75.00% vs.41.51%), the quality of life after chemotherapy cycle ( 90.86 ± 2.55 vs.78.04 ± 2.21 ), and the quality of life after 1 year of follow-up ( 85.68 ± 2.23 vs.70.27 ± 1.72 ) in the observation group were significantly higher than those in the control group, and the difference was statistically significant ( p &lt; 0.05 ).The incidence of adverse reactions (9.57% vs.20.24%), mortality (10.64% vs.21.43%), and recurrence rate (11.70% vs.27.38%) during the follow-up period in the observation group were significantly lower than those in control group, and the differences were statistically significant ( p &lt; 0.05 ).Conclusion.Dsa-guided bace interventional therapy for patients with primary middle-advanced bronchial lung cancer has significant efficacy, which can not only reduce the mortality and recurrence rate of patients but also improve the quality of life of patients, with fewer adverse reactions and high safety, which is worthy of promotion.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;