syedzayyan/paper_mill_benchmark huggingface dataset:
Amphotericin B and Curcumin Co-Loaded Porous Microparticles as a Sustained Release System againstCandida albicans.

Amphotericin b (amb) is an antifungal drug used for serious fungal infections.However, amb has adverse reactions such as nephrotoxicity, which limit the clinical application of amb alone or in combination with other antifungal drugs.Nano or micro drug delivery systems (dds) have been proven to be effective in reducing the toxic and side effects of drugs.Further, the combination of amb with other compounds with antifungal activity, such as curcumin (cm), may enhance the synergistic effects.Herein, amb and cm were co-loaded into porous poly (lactic-co-glycolic acid) (plga) microparticles (mps) to prepare amb/cm-plga mps.The amb/cm-plga mps showed a remarkably reduced hemolysis (62.2 ± 0.6%) compared to amb (80.9 ± 1.1%).The nephrotoxicity of amb/cm-plga mps is significantly lower than that of amb.In vitro, amb/cm-plga mps had better inhibitory effects on the adhesion and biofilm formation of candida albicanscompared with amb.Experiments on mice infected with c.Albicansshowed that amb/cm-plga mps have a better therapeutic effect than amb in vivo.In summary, amb/cm-plga mps may be a novel and promising therapeutic candidate for fungal infection.

Journal: Molecules (Basel, Switzerland) (2022)
Publication types: Journal Article
Effect of Fast High-Irradiance Photo-Polymerization of Resin Composites on the Dentin Bond Strength.

This study investigated the influence of conventional (10 s at 1160 m w/cm2) and fast high-irradiance (3 s at 2850 m w/cm2) light curing on the micro-tensile bond strength (μtbs) of bulk-fill resin composites bonded to human dentin.Sixty-four extracted human molars were ground to dentin and randomly assigned into eight groups (n = 8 per group).After application of a three-step adhesive system (optibond fl), four different bulk-fill composites (two sculptable and two flowable composites) were placed.Of these, one sculptable (tetric power fill) and one flowable (tetric power flow) composite were specifically developed for fast high-irradiance light curing.Each composite was polymerized with the conventional or the fast high-irradiance light-curing protocol.The specimens were cut into dentin-composite sticks, μtbs was determined and failure modes were analyzed.Statistical analysis was performed usingt-test for independent observations and one-way anova.A statistical difference between the curing protocols was only found for tetric power flow, where the conventional protocol (23.8 ± 4.2 mpa) led to significantly higher values than the fast high-irradiance light-curing protocol (18.7 ± 3.7 mpa).All other composite materials showed statistically similar values for both polymerization protocols.In conclusion, the use of fast high-irradiation light curing has no negative influence on the μtbs of the investigated high-viscosity bulk-fill composites.However, it may reduce the dentin bond strength of flowable bulk-fill composite.

Journal: Materials (Basel, Switzerland) (2022)
Publication types: Journal Article
Biosynthesis of bacterial cellulose nanofibrils in black tea media by a symbiotic culture of bacteria and yeast isolated from commercial kombucha beverage.

Bacterial cellulose has drawn the attention for its unique properties and applications including; medicine, pharmacy, food, agricultural, textile and electronics.The present study focused on the production of bacterial cellulose nanofibrils (bcnf) from black tea as cost effective alternative medium in addition to study the effect of gamma radiation on bcnf properties.A symbiotic culture of bacteria and yeast (scoby) were isolated from commercial kombucha beverage and were identified as acinetobacter lowffii and candida krusei, respectively.The symbiotic culture was used for production of bcnf on hestrin-schramm (hs), black tea (bt) and modified bt media.Bcnf was purified (0.5 n na oh) and quantified by dry weight, yield and productivity determination.Characterization and effect of gamma radiation (5-25 k gy) on bcnf were studied using scanning electron microscope (sem), fourier transform infrared (ftir) and x-ray diffraction (xrd).The highest bcnf production was achieved using bt medium with 0.2% tea and 6.0% commercial sugar (with dry weight 4.77-4.61 g/l and productivity 68.14% and 65.85%, respectively).Supplementation of bt medium with 1% ethanol, 0.27% na2hpo4and 0.5% yeast extract individually, enhanced the bcnf production (7.85, 6.84 and 5.73 g/l), respectively.Ftir spectrum of bcnf from sugared water (sw), hs and bt showed similar structure with high purity.As a conclusion, gamma irradiation has no effect on the bcnf structure while showed different effects on its crystallinity index and size with the different doses.The changes in cr i were ranged between (17 and 23.5%), while the crystallinity size (cs) was affected by gamma irradiation in a positive relationship where the crystalline size was decreased (33%) by exposure to 5 k gy then increased by increasing the dose of radiation reaching 25.7% at 25 k gy.Sem graphs showed the morphology of microbial culture and its symbiotic relationship in addition to the ultrafine structure of non-irradiated and irradiated bcnf.

Journal: World journal of microbiology & biotechnology (2022)
Publication types: Journal Article
Overexpression of transcription factor FoxA2 in the developing skeleton causes an enlargement of the cartilage hypertrophic zone, but it does not trigger ectopic differentiation in immature chondrocytes.

We previously found that fox a factors are necessary for chondrocyte differentiation.To investigate whether fox a factors alone are sufficient to drive chondrocyte hypertrophy, we build a fox a2 transgenic mouse in which fox a2 c dna is driven by a reiterated tetracycline response element (tre) and a minimal cmv promoter.This transgenic line was crossed with a col2cre;rosa26rt ta/+mouse line to generate col2cre;rosa26rt ta/+;tg fox a2+/-mice for inducible expression of fox a2 in cartilage using doxycycline treatment.Ectopic expression of fox a2 in the developing skeleton reveals skeletal defects and shorter skeletal elements in e17.5 mice.The chondro-osseous border was frequently mis-shaped in mutant mice, with small islands of col.10+ hypertrophic cells extending in the metaphyseal bone.Even though overexpression of fox a2 causes an accumulation of hypertrophic chondrocytes, it did not trigger ectopic hypertrophy in the immature chondrocytes.This suggests that fox a2 may need transcriptional co-factors (such as runx2), whose expression is restricted to the hypertrophic zone, and absent in the immature chondrocytes.To investigate a potential fox a2/runx2 interaction in immature chondrocytes versus hypertrophic cells, we separated these two subpopulations by facs to obtain cd24+cd200+hypertrophic chondrocytes and cd24+cd200-immature chondrocytes and we ectopically expressed fox a2 alone or in combination with runx2 via lentiviral gene delivery.In cd24+cd200+hypertrophic chondrocytes, fox a2 enhanced the expression of chondrocyte hypertrophic markers collagen 10, mmp13, and alkaline phosphatase.In contrast, in the cd24+cd200-immature chondrocytes, neither fox a2 nor runx2 overexpression could induce ectopic expression of hypertrophic markers mmp13, alkaline phosphatase, or pth/pthr p receptor.Overall these findings mirror our in vivo data, and suggest that induction of chondrocyte hypertrophy by fox a2 may require other factors in addition to runx2 (i.e., hif2α, mef2c, or perhaps unknown factors), whose expression/activity is rate-limiting in immature chondrocytes.

Journal: Bone (2022)
Publication types: Journal Article
A phased approach for the adaptation of telemedicine in arrythmia management.

Cardiovascular disease is the world's leading cause of death.People at higher risk of heart disease need early detection to prevent or delay serious disease complications.In this paper, we present a new design of a wireless portable ecg (electrocardiogram).The ecg signal was preprocessed by analog filters and amplifiers before it was converted into digital data via an arduino board, a highly available device for real-time signal processing.In order to allow communication between arduino and android phone, we use a bluetooth module.The proposed mobile telemedicine technique allows immediate and rapid detection of heart defects followed by an alarm message from the patient to the doctor.

Journal: The Review of scientific instruments (2021)
Publication types: Journal Article
Structural and Morphological Analysis of the First Alloy/Dealloy of a Bulk Si-Li System at Elevated Temperature.

There have been tremendous improvements in the field of si electrode materials, either by nanoscale or composite routes, and though silicon-containing carbon electrode materials have begun to penetrate the marketplace, the commercial capacities achieved by these cells still fall short of the promise of high capacity si electrodes.Enabling a cheaper feedstock of si in the bulk form would make this technology more accessible, though there are many challenges that must be overcome.Whereas other methods utilize nanomaterials and composites to overcome volume expansion and pulverization of a si electrode, this study explores a thermal route to enable the use of carbon-free bulk si.To accomplish this, a modified swagelok cell has been constructed to accommodate high temperatures, corrosive molten salt electrolytes, and a molten lithium electrode to study lithiation of a bulk si wafer at 250 °c.Scanning electron microscopy, x-ray diffraction, and microcomputed tomography were used to examine morphological and structural changes within the si upon lithiation and delithiation.It was discovered that semiordered lix si phases were formed upon lithiation in molten li tfsi electrolyte at 250 °c, and the higher temperature does not completely mitigate pulverization of the bulk si electrode.

Journal: ACS omega (2022)
Publication types: Journal Article
An impaired metabolism of nucleotides underpins a novel mechanism of cardiac remodeling leading to Huntington's disease related cardiomyopathy.

Huntington's disease (hd) is mainly thought of as a neurological disease, but multiple epidemiological studies have demonstrated a number of cardiovascular events leading to heart failure in hd patients.Our recent studies showed an increased risk of heart contractile dysfunction and dilated cardiomyopathy in hd pre-clinical models.This could potentially involve metabolic remodeling, that is a typical feature of the failing heart, with reduced activities of high energy phosphate generating pathways.In this study, we sought to identify metabolic abnormalities leading to hd-related cardiomyopathy in pre-clinical and clinical settings.We found that hd mouse models developed a profound deterioration in cardiac energy equilibrium, despite amp-activated protein kinase hyperphosphorylation.This was accompanied by a reduced glucose usage and a significant deregulation of genes involved in de novo purine biosynthesis, in conversion of adenine nucleotides, and in adenosine metabolism.Consequently, we observed increased levels of nucleotide catabolites such as inosine, hypoxanthine, xanthine and uric acid, in murine and human hd serum.These effects may be caused locally by mutant htt, via gain or loss of function effects, or distally by a lack of trophic signals from central nerve stimulation.Either may lead to energy equilibrium imbalances in cardiac cells, with activation of nucleotide catabolism plus an inhibition of re-synthesis.Our study suggests that future therapies should target cardiac mitochondrial dysfunction to ameliorate energetic dysfunction.Importantly, we describe the first set of biomarkers related to heart and skeletal muscle dysfunction in both pre-clinical and clinical hd settings.

Journal: Biochimica et biophysica acta (2016)
Publication types: Journal Article
Influence of a Commercial Biological Fungicide containingTrichoderma harzianumRifai T-22 on Dissipation Kinetics and Degradation of Five Herbicides in Two Types of Soil.

Biological crop protection is recommended to be applied alternately or together with chemical one, to protect human health from the excessive use of toxic pesticides.Presence of microorganisms can influence the concentration of chemical pollutants in soil.The aim of this study is to estimate the influence of a commercial biological fungicide containing trichoderma harzianum rifai t-22 on dissipation kinetics and degradation of five herbicides belonging to different chemical classes: clomazone, fluazifop-p-butyl, metribuzin, pendimethalin, and propyzamide, in two types of soil.Results of the study revealed that t.Harzianum t-22 influences pesticide degradation and dissipation kinetics of the non-persistent herbicides: clomazone, fluazifop-p-butyl, and metribuzin.In soil with a higher content of nitrogen, phosphorus, and organic matter, degradation increased by up to 24.2%, 24.8%, and 23.5% for clomazone, fluazifop-p-butyl, and metribuzin, respectively.In soil with lower organic content, degradation was on a low level, of 16.1%, 17.7%, and 16.3% for clomazone, fluazifop-p-butyl, and metribuzin, respectively.In our study, the addition of the biological preparation shortened herbicide dissipation half-lives, from 0.3 days (2.9%) for fluazifop-p-butyl, to 18.4 days (25.1%) for clomazone.During the degradation study, no significant differences were noticed for pendimethalin, belonging to persistent substances.Biological protection of crops can modify pesticide concentrations and dissipation rates.On one hand, this may result in the reduced effectiveness of herbicide treatments, while on the other, it can become a tool for achieving cleaner environment.

Journal: Molecules (Basel, Switzerland) (2020)
Publication types: Journal Article
Numerical modeling of a self-propelled dolphin jump out of water.

A computational model is developed to investigate the jump of a self-propelled dolphin out of water.This model relies on the navier-stokes equations, where a fictitious domain approach with the volume penalization method is used for fluid-structure coupling, and the continuous surface force approach is used to model the water-air interface, the latter being tracked in a level-set framework.The dolphin's geometry is based on freely available data from the literature.While body deformation is imposed, the leading linear and angular displacements are computed from newton's laws.Numerical simulations show that it is necessary to generate large propulsives forces to allow the jump out of water.When the dolphin is out of water, its trajectory follows a purely ballistic one.

Journal: Bioinspiration & biomimetics (2022)
Publication types: Journal Article
Assessment of microalgae as a new feeding additive for fruit fly Drosophila melanogaster.

Animal food wastes are a concern due to the large amounts of commercial food required for model animals during the biological and biomedical research.Searching for sustainable food alternatives with negligible physiological effects on animals is critical to solve or reduce this challenge.Microalgae have been demonstrated to be suitable for both human consumption and animal feed.In this study, the possibility of using chlorella vulgaris and senedesmus obliquus as a feed replacement to drosophila melanogaster, one of the fly models commonly used in biomedical studies, was investigated.Characteristics including the fly locomotor activity, motor pattern, feeding behavior, lifespan and body weight were assessed.Results showed that compared to control, the flies fed on 80% microalga (80-flies) in the total weight (w/w) had the double increased apparent step size, while both 60-flies and 80-flies exhibited longer travel distances (60%: 27.77 ± 1.99 cm; 80%: 31.50 ± 3.70 cm) most likely due to the starvation and varied serotonin levels in flies fed on high percentages microalgae.Subsequently, 40-flies exhibited less optimal growth performance with decreased body weights (0.51 ± 0.006 mg vs 0.60 ± 0.005 mg for control) and shorter mean lifespan (36 days vs 55.8 days for control.However, 20-flies showed no statistical differences in all parameters tested with respect to control flies, indicating that 20% microalgae treatment did not greatly change the primary food component such as carbohydrate which might play a critical role in fly performance.Therefore, the inclusion of 20% microalgae could be an alternative to fly standard food without compromising fly physiological performance.

Journal: The Science of the total environment (2019)
Publication types: Journal Article
Influences of remote ischemic preconditioning on postoperative delirium and cognitive dysfunction in adults after cardiac surgery: a meta-analysis of randomized controlled trials.

Remote ischemic preconditioning (ripc) has been suggested to confer neuroprotective effect.However, influences of ripc on postoperative delirium (pod) and cognitive dysfunction (pocd) in adults after cardiac surgery are less known.We performed a meta-analysis of randomized controlled trials (rcts) to evaluate the effects of ripc on pod and pocd.Methods relevant studies were obtained by search of pub med, embase, and cochrane's library databases.A random-effect model was used to pool the results.Results ten rcts including 2303 adults who received cardiac surgery were included.Pooled results showed that ripc did not significantly affect the incidence of pod (six rcts, odds ratio [or] 1.07, 95% confidence interval [ci] 0.81 to 1.40, p = 0.65) with no significant heterogeneity (i2= 0%).In addition, combined results showed that ripc did not significantly reduce the incidence of pocd either (six rcts, or 0.64, 95% ci 0.37 to 1.11, p = 0.11) with moderate heterogeneity (i2= 44%).Sensitivity analysis by excluding one rct at a time showed consistent results (p values all > 0.05).Conclusions current evidence from rcts did not support that ripc could prevent the incidence of pod or pocd in adults after cardiac surgery.Although these findings may be validated in large-scale rcts, particularly for the results of pocd, based on these findings, ripc should not be routinely used as a preventative measure for pod and pocd in adult patients after cardiac surgery.

Journal: Perioperative medicine (London, England) (2021)
Publication types: Journal Article
Conscious and Unconscious Bias: The Hidden Pandemic of Biases in Healthcare Exacerbated by COVID-19.

There are limited data on unconscious bias in healthcare, but there is consistent evidence that it alters clinical decision-making.Covid-19 exacerbated many pre-existing disparities, and this paper seeks to identify, deconstruct, and propose mitigation strategies for a few of them.Discussion five of the largest disparities amplified by the pandemic are discussed in this paper.Older people, black people, uninsured people, rural communities, and people with lower education levels have been disproportionally affected in both morbidity and mortality.Conclusions the disparities discussed above did not occur in a vacuum but are the result of systemic issues.Equity starts with understanding and addressing the root cause, and it can be worked toward with practical and impactful solutions.

Journal: HCA healthcare journal of medicine (2022)
Publication types: Journal Article;Review
Complete Genome Sequence ofMicrobacteriumsp. Strain SGAir0570, Isolated from Tropical Air Collected in Singapore.

Microbacteriumsp.Strain sgair0570 was isolated from air samples collected in singapore.Its genome was assembled using single-molecule real-time sequencing and mi seq short reads.It has one chromosome with a length of 3.38 mb and one 59.2-kb plasmid.It contains 3,170 protein-coding genes, 48 t rnas, and 6 r rnas.

Journal: Microbiology resource announcements (2019)
Publication types: Journal Article
The Histopathology of Neoadjuvant-Treated (NAT) Pancreatic Ductal Adenocarcinoma.

Examination of pancreatic ductal adenocarcinoma after nat with the intent of diagnosis and outcome prediction remains a challenging task.The lack of a uniform approach to macroscopically assess these cases along with variations in sampling adds to the complexity.Several trg systems have been proposed to correlate with an overall survival.In clinical practice, most of these trg schemes have shown low level of interobserver agreement arguing for a need of larger studies and more innovative ways to assess outcome in this population.

Journal: Surgical pathology clinics (2022)
Publication types: Journal Article;Review
Cancer cell metabolism as new targets for novel designed therapies.

Metabolic processes are altered in cancer cells, which obtain advantages from this metabolic reprogramming in terms of energy production and synthesis of biomolecules that sustain their uncontrolled proliferation.Due to the conceptual progresses in the last decade, metabolic reprogramming was recently included as one of the new hallmarks of cancer.The advent of high-throughput technologies to amass an abundance of omic data, together with the development of new computational methods that allow the integration and analysis of omic data by using genome-scale reconstructions of human metabolism, have increased and accelerated the discovery and development of anticancer drugs and tumor-specific metabolic biomarkers.Here we review and discuss the latest advances in the context of metabolic reprogramming and the future in cancer research.

Journal: Future medicinal chemistry (2014)
Publication types: Journal Article;Research Support, Non-U.S. Gov't;Review
Peroxidase-like Active Nanomedicine with Dual Glutathione Depletion Property to Restore Oxaliplatin Chemosensitivity and Promote Programmed Cell Death.

The nanocatalytic activity of nanozymes provides a vision for tumor treatment.However, the glutathione (gsh)-related antioxidant defense system (ads) formed on the basis of excessive gsh in the tumor microenvironment limits its catalytic activity.Here, dendritic mesoporous silica nanoparticles (dmsns) were employed as nanocarrier; ultrasmall fe3o4nanoparticles, mn2+ions, and glutaminase inhibitor telaglenastat (cb-839) were subsequently integrated into large mesopores of dmsns, forming dmsn/fe3o4-mn@cb-839 (dfmc) nanomedicine.This nanomedicine exhibits peroxidase mimicking activities under acidic conditions, which catalyzes the decomposition of hydrogen peroxide (h2o2) into hydroxyl radical (•oh).This also promotes the formation of lipid peroxides, which is required for ferroptosis.Furthermore, this nanomedicine can effectively deplete the existing gsh, thereby enhancing reactive oxygen species (ros)-mediated tumor catalytic therapy.Moreover, the introduced cb-839 blocks the endogenous synthesis of gsh, further enhancing gsh depletion performance, which reduces the excretion of oxaliplatin (gsh-related resistance) from tumor cells, thereby restoring the chemical sensitivity of oxaliplatin.The dual gsh depletion property significantly weakens the gsh-related ads and restores the chemical sensitivity of oxaliplatin, leading to the high dfmc-induced apoptosis and ferroptosis of tumor cells.Our developed nanomedicine based on integrated nanotechnology and clinical drug may aid the development of tumor treatment.

Journal: ACS nano (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't