syedzayyan/paper_mill_benchmark huggingface dataset:
Notoginsenoside R1 Protects HUVEC Against Oxidized Low Density Lipoprotein (Ox-LDL)-Induced Atherogenic Response via Down-Regulating miR-132

Radix notoginseng is a well-known traditional chinese herbal medicine, has extensively pharmacological activities in cardiovascular system.Notoginsenoside r1 (ngr1) is one main active ingredient of radix notoginseng.The purpose of this study was to evaluate the functional effects of ngr1 on atherosclerosis (as).Methods: human umbilical vascular endothelial cells (huvecs) were subjected to oxidized low density lipoprotein (ox-ldl), before which cells were preconditioned with ngr1.Cell counting kit-8 (cck-8) assay, flow cytometry, transwell assay, quantitative real-time polymerase chain reaction (q rt-pcr) and western blot were carried out to assess the impacts of ox-ldl and ngr1 on huvecs.Besides, the expression of micro rna-132 (mi r-132), and the regulatory role of mi r-132 in matrix gla protein (mgp) expression were measured by q rt-pcr and western blot.Results: ngr1 pre-conditioning prevented ox-ldl-induced apoptosis, migration and overproduction of monocyte chemoattractant protein 1 (mcp-1) and intercellular adhesion molecule 1 (icam-1).Mi r-132 was up-regulated in response to ox-ldl while was down-regulated by ngr1 pre-conditioning.The protective actions of ngr1 in ox-ldl-treated huvecs were enhanced by mi r-132 inhibitor, while were attenuated by mi r-132 mimic.Besides, the up-regulated mi r-132 could further decrease the expression of mgp, which acted as an anti-migratory and anti-adhesive factor.Furthermore, ox-ldl-induced the activation of c-jun n-terminal kinase (jnk) and nuclear factor kappa b (nf-κb) pathways were partially attenuated by ngr1, and were fully eliminated by ngr1 treatment together with mgp overexpression.Conclusion: ngr1 prevents ox-ldl-induced apoptosis, migration and adhesion-related molecule release in huvecs possibly via down-regulating mi r-132, and subsequent up-regulating mgp.

Journal: Cellular Physiology and Biochemistry (2018)
Publication types: Research Article;
circZMYM2 Competed Endogenously with miR-335-5p to Regulate JMJD2C in Pancreatic Cancer

We aimed to study the involvement of circ zmym2 (hsa_circ_0099999) in pancreatic cancer (pc) cell proliferation, apoptosis and invasion and to figured out the underlying mechanism of circ zmym2 regulating mi r-335-5p and jmjd2c.Methods: circ rna differential expressions in twenty pc samples and paired normal tissue samples were analyzed using arraystar human circ rna microarray v1.Circ zmym2 expression level was determined via q rt-pcr.The effects of circ zmym2 inhibition and overexpression on cell proliferation, cell apoptosis and cell invasion were investigated by cck-8 assays, flow cytometry assays and transwell assays.An animal experiment on nude mice was put forward to test the influence of circ zmym2 knockdown on tumor growth.The relationship between circ zmym2, mi r-335 and jmjd2c was verified by rna pull down, dual-luciferase reporter assays and rescue experiment.The effect of circ zmym2 and mi r-335-5p on the expression of jmjd2c protein was detected by western blot.Results: circ zmym2 overexpression was observed in both pc tissues and cells.Knockdown of circ zmym2 inhibited proliferation, induced apoptosis, and weakened invasion ability of cancer cells.Tumor growth was restrained in vivo.Circ zmym2 repressed the expression of its target mi r-335-5p.Mi r-335-5p attenuated pancreatic cancer development via inhibition of jmjd2c.Conclusion: our study demonstrated that circ zmym2 promoted pc progression.Circ zmym2 had a sponge effect on mi r-335-5p and modulated the downstream oncogene jmjd2c.

Journal: Cellular Physiology and Biochemistry (2018)
Publication types: Research Article;
Sirtuin 6 contributes to migration and invasion of osteosarcoma cells via the ERK1/2/MMP9 pathway

Dysregulation of sirtuin 6 (sirt6) is actively involved in tumor progression.High levels of sirt6 have been associated with hepatocellular carcinoma and non‐small cell lung cancer, and sirt6 facilitates growth and metastasis of cancer cells.However, the clinical significance and biological function of sirt6 are not known for osteosarcoma (os).Here, we report that sirt6 was notably overexpressed in os tissues compared with non‐cancerous specimens.The high level of sirt6 was prominently correlated with malignant clinical parameters and poor prognosis of os patients.Sirt6 was also up‐regulated in os cells.Sirt6 knockdown inhibited the invasion and migration of saos‐2 and u2os cells in vitro , while sirt6 restoration increased these cellular biological behaviors in mg‐63 cells.Mechanistically, sirt6 up‐regulated expression of matrix metallopeptidase 9 (mmp9) in os cells.Mmp9 restoration partially abolished the effects of sirt6 knockdown on os cells, with increased cell migration and invasion.Mmp9 knockdown reduced migration and invasion of sirt6‐overexpressing mg‐63 cells.Furthermore, sirt6 positively modulated the levels of phosphorylated extracellular signal‐regulated kinases 1 and 2 (erk1/2).Pd098059 and pd0325901, inhibitors of mitogen‐activated protein kinase kinase (mek), blocked the regulatory effects of sirt6 on p‐erk1/2 and mmp9 levels, suggesting that sirt6 regulated mmp9 abundance probably through the mek–erk1/2 pathway.These results suggest that sirt6 may act as a prognostic predictor and a drug target for os patients.

Journal: FEBS Open Bio (2017)
Publication types: Research Article;
The PI3K/AKT axis modulates AATF activity in Wilms' tumor cells

Previous studies have reported excessive expression of apoptosis‐antagonizing transcription factor ( aatf ) in various tumors, where it reinforces the generation and development of cancers and is linked to the clinical outcome.Nevertheless, the expression and influence of aatf in wilms’ tumor ( wt ) is largely unknown.Here, we discovered that aatf expression was markedly increased in wt tissues as compared to the surrounding normal tissues.Elevated levels of aatf expression were related to tumor relapse and pulmonary metastasis, congruent with it being a predictor of clinical outcome in people suffering from wt .Proliferation, invasion, and migration of wt cells were suppressed by knockdown of aatf and promoted by aatf overexpression in vitro .Furthermore, the tumor generation capability of wt cells noticeably decreased after knockout of aatf in vivo .The phosphoinositide‐3‐kinase ( pi 3k)/ akt pathway modulated the activity of aatf in wt .The findings of our study indicate that aatf expression is increased in wt and can serve as a predictor of clinical outcome; in addition, it may enhance the development of wt via the pi 3k/ akt axis and may be a promising marker for wt diagnosis and therapy.

Journal: FEBS Open Bio (2018)
Publication types: Research Article;
microRNA-497 inhibits cell proliferation and induces apoptosis by targeting YAP1 in human hepatocellular carcinoma

Micro rna s (mi rna s) function as oncogenes or tumor suppressors in human cancers by targeting m rna s for degradation and/or translational repression.Mi r‐497 has been proposed as a tumor suppressive mi rna and its deregulation is observed in human cancers.However, the prognostic value of mi r‐497 and its underlying molecular pathways involved in the initiation and development of hepatocellular carcinoma ( hcc ) are poorly investigated.In the present study, we found that the mean level of mi r‐497 in hcc tissues was lower than that in adjacent nontumor tissues.Clinical data indicated that low expression of mi r‐497 was prominently associated with adverse prognostic features of hcc including high serum alpha‐fetoprotein ( afp ) level, large tumor size, high edmondson–steiner grading and advanced tumor–node–metastasis ( tnm ) stage.Furthermore, mi r‐497 was an independent prognostic factor for indicating both 5‐year overall survival and disease‐free survival of hcc patients.Gain‐ and loss‐of‐function studies showed that mi r‐497 reduced cell proliferation and induced apoptosis in hcc cells.Yes‐associated protein 1 ( yap 1) was identified as a direct target of mi r‐497 in hcc .An inverse correlation between yap 1 and mi r‐497 expression was observed in hcc tissues.Notably, yap 1 knockdown abrogated the effects of mi r‐497 deletion on hcc cells with decreased cell proliferation and increased apoptosis.In conclusion, we report that mi r‐497 is a potent prognostic indicator and may suppress tumor growth of hcc by targeting yap 1.

Journal: FEBS Open Bio (2016)
Publication types: Research Article;
Ubiquitin ligase SMURF1 functions as a prognostic marker and promotes growth and metastasis of clear cell renal cell carcinoma

Smad ubiquitin regulatory factor 1 ( smurf 1), a recently identified e3 ubiquitin ligase, targets substrate proteins for ubiquitination and proteasomal degradation.Previous studies have reported that smurf 1 also functions as an oncogene in human cancers.However, the clinical value of smurf 1 and its role in clear cell renal cell carcinoma (cc rcc ) are unknown.Smurf 1 expression was analyzed in 100 cases of cc rcc and matched tumor‐adjacent specimens.Smurf 1 was prominently overexpressed in cc rcc specimens compared with tumor‐adjacent specimens.Increased levels of smurf 1 were also observed in cc rcc cell lines.Clinicopathological detection verified that smurf 1 expression was associated with advanced tumor node metastasis stage, large tumor size and vascular invasion of cc rcc patients.Moreover, kaplan–meier analysis found that smurf 1 elevation led to adverse overall survival and disease‐free survival.Multivariate cox regression analysis revealed that smurf 1 expression was an independent marker for prognosis prediction.Further experiments illustrated that smurf 1 knockdown significantly inhibited growth and metastasis of 769p cells, while smurf 1 overexpression promoted proliferation, migration and invasion in osrc ‐2 cells.Mechanistically, smurf 1 inversely regulated the expression of dab 2 interacting protein, which negatively mediated the activation of both the erk / rsk 1 and pi 3k/ akt / m tor pathways in cc rcc cells.Taken together, these results suggest that smurf 1 might be a promising biomarker and target for novel treatment of human cc rcc.

Journal: FEBS Open Bio (2017)
Publication types: Research Article;
miR-944 inhibits metastasis of gastric cancer by preventing the epithelial-mesenchymal transition via MACC1/Met/AKT signaling

Micro rna s (mi rna s) are reported to play vital roles in tumor progression.Recently, mi r‐944 was reported to play either an oncogenic or tumor suppressive role in human cancers.However, the expression of mi r‐944 and its exact role in gastric cancer ( gc ) remain unknown.This study aimed to evaluate whether loss of mi r‐944 could promote the epithelial–mesenchymal transition ( emt ) of gc .Reduced expression of mi r‐944 was identified in 40 pairs of human gc and matched normal tissues by q rt ‐ pcr .Reduced expression of mi‐944 was also observed in gc cell lines.Restoration of mi r‐944 inhibited cell migration and invasion in mgc ‐803 cells, while its loss facilitated metastasis of sgc ‐7901 and bgc ‐823 cells.Notably, mi r‐944 overexpression prohibited emt of gc cells in vitro , while mi r‐944 knockdown had the opposite effect.Bioinformatics software predicted that macc 1 was a direct target of mi r‐944.We observed negative regulation of mi r‐944 on macc 1 expression, and direct binding between mi r‐944 and macc 1 was verified by dual‐luciferase assays in hek 293t cells.Restoration of macc 1 resulted in promoted emt and metastasis in mi r‐944‐overexpressing mgc ‐803 cells.Loss of macc 1 abrogated the effects of mi r‐944 knockdown on emt and metastasis of sgc ‐7901 cells.We also found that the met– akt pathway might be involved in macc 1‐mediated emt .In conclusion, mi r‐944 acts as an inhibitor of emt and metastasis of gc by targeting macc 1.This study highlights the potential effects of mi r‐944 in the prognosis and treatment of gc .

Journal: FEBS Open Bio (2017)
Publication types: Research Article;
High-intensity focused ultrasound inhibits invasion and metastasis of colon cancer cells by enhancing microRNA-124-mediated suppression of STAT3

Metastasis is the primary contributor to colorectal cancer mortality.High‐intensity focused ultrasound (hifu) is an emerging technology for tumor therapy that exerts its effects through tumor ablation, mechanical disruption, and enhancement of immune responses.However, it remains unclear whether hifu can influence tumor metastasis.Here, we examined the effect of hifu on tumor metastasis of colorectal cancer cells and the underlying mechanisms.Hifu was observed to inhibit migration of hct‐116 cells in vitro and suppress lung metastasis in a mouse model of colon cancer.In addition, hifu up‐regulated micro rna (mi r) ‐124 expression, which inhibited the activation of signal transducer and activator of transcription 3 (stat3) and inhibited migration of hct‐116 cells.Treatment with an inhibitor of mi r‐124 reversed the effect of hifu on cell migration.In conclusion, our results suggest that hifu exerts anti‐metastatic effects in colon cancer, and this effect is possibly mediated via up‐regulation of mi r‐124 and subsequent mi r‐124‐mediated stat3 suppression.

Journal: FEBS Open Bio (2019)
Publication types: Research Article;
Knockdown of lncRNA MNX1-AS1 suppresses cell proliferation, migration, and invasion in prostate cancer

Altered expression of long non‐coding rna s (lnc rna s) has been reported in many malignancies, including prostate cancer.However, the role of lnc rna mnx 1‐ as 1 in prostate cancer has not been reported.Here, we report that mnx 1‐ as 1 is expressed in prostate cancer tissues and cells and that si rna ‐mediated knockdown of mnx 1‐ as 1 inhibits proliferation, migration, and invasion of prostate cancer du 145 and pc 3 cells.In addition, down‐regulation of mnx 1‐ as 1 decreased expression of proliferating cell nuclear antigen, ph ‐3, n‐cadherin, and vimentin, but enhanced expression of e‐cadherin.In conclusion, this is the first report that knockdown of mnx 1‐ as 1 suppresses prostate cancer cell proliferation, migration, and invasion.We believe that mnx 1‐ as 1 may be a potential new therapeutic target for prostate cancer patients.

Journal: FEBS Open Bio (2019)
Publication types: Research Article;
Lymphocyte-specific protein 1 inhibits the growth of hepatocellular carcinoma by suppressing ERK1/2 phosphorylation

Lymphocyte‐specific protein 1 ( lsp 1) has been reported to regulate cell biology in several human cancers including lymphoma and breast cancer.However, the functions of lsp 1 in human hepatocellular carcinoma ( hcc ) are still unknown.In this study, we found that lsp 1 expression was downregulated in hcc tissues and cell lines, and lower lsp 1 expression was correlated with poor clinicopathological features including large tumor size, high edmondson–steiner grading and advanced tumor–node–metastasis ( tnm ) stage.Additionally, we demonstrated that patients with high lsp 1 expression had significantly better overall survival and disease‐free survival.Moreover, lsp 1 was found to be an independent factor for predicting the prognosis of hcc patients.In vitro and in vivo assays showed that overexpressing lsp 1 inhibited hcc growth by inducing both apoptosis and growth arrest.Mechanistically, we found that expression of phosphorylated extracellular regulated protein kinases 1 and 2 ( erk 1/2) was downregulated after lsp 1 overexpression, indicating lsp 1 could suppress hcc growth by inhibiting the erk pathway in hcc cells.Taken together, these results indicate that lsp 1 may serve as a prognostic marker and a potential therapeutic target in human hcc .

Journal: FEBS Open Bio (2016)
Publication types: Research Article;
MicroRNA-520c enhances cell proliferation, migration, and invasion by suppressing IRF2 in gastric cancer

Dysregulation of micro rna (mi rna ) is actively involved in the development and progression of gastric cancer ( gc ).Mi r‐520c was previously found to be overexpressed in gc specimens and cells.However, the clinical significance of mi r‐520c and its biological function in gc remain largely unknown.Here, we found that mi r‐520c expression in gc tissues was significantly increased compared to normal adjacent gastric tissues.Its increased level was prominently correlated with poor clinical parameters and prognosis of gc patients.Accordingly, the expression of mi r‐520c was obviously elevated in gc cell lines as compared with gastric epithelial cells.Overexpression of mi r‐520c in n‐87 cells significantly increased the proliferative ability, migration, and invasion of cancer cells, while mi r‐520c silencing suppressed mkn ‐45 cell proliferation, migration, and invasion in vitro .Mechanically, mi r‐520c inversely regulated interferon regulatory factor 2 ( irf 2) abundance in gc cells.Herein, irf 2 was found to be a downstream target of mi r‐520c in gc .Furthermore, irf 2 was down‐regulated in gc tissues compared to nontumor tissues.An inverse correlation between irf 2 and mi r‐520c expression was observed in gc cases.Taken together, mi r‐520c may serve as a prognostic predictor and a therapeutic target for gc patients.

Journal: FEBS Open Bio (2016)
Publication types: Research Article;
MicroRNA-15b shuttled by bone marrow mesenchymal stem cell-derived extracellular vesicles binds to WWP1 and promotes osteogenic differentiation

Background osteogenic differentiation is an essential process for bone regeneration involving bone marrow mesenchymal stem cells (bmscs).Bmsc-secreted extracellular vesicles (evs) enriched with micro rnas (mi rs) have vital roles to play in mediating osteogenic differentiation.Therefore, this study aimed to explore the effect of bmsc-derived evs loaded with mi r-15b on osteogenic differentiation.Methods human bmscs (h bmscs) were cultured and treated with plasmids overexpressing or knocking down klf2, wwp1, and mi r-15b to define the role of derived evs in osteogenic differentiation in vitro.The expression of osteogenic differentiation-related marker was measured by western blot analysis.The interaction among mi r-15b, wwp1, and ubiquitination of klf2 was investigated by dual-luciferase reporter, immunoprecipitation, and gst pull-down assays.Moreover, evs from h bmscs transfected with mi r-15b inhibitor (ev-mi r-15b inhibitor) were injected into ovariectomized rats to verify the effect of mi r-15b on bone loss in vivo.Results wwp1 was downregulated, and klf2 was upregulated during osteogenic differentiation.After co-culture with evs, mi r-15b expression was elevated and wwp1 expression was reduced in h bmscs.Upregulation of mi r-15b or klf2 or downregulation of wwp1 or nf-κb increased alp activity and cell mineralization, as well as osteogenic differentiation-related marker expression in h bmscs.Mechanistically, mi r-15b targeted and inhibited wwp1, thus attenuating klf2 degradation and inhibiting nf-κb activity.Co-culture of evs increased the bone volume and trabecular number, but decreased bone loss in ovariectomized rats, which could be reversed after treatment with ev-mi r-15b inhibitor.Conclusion collectively, bmsc-derived evs loaded with mi r-15b promoted osteogenic differentiation by impairing wwp1-mediated klf2 ubiquitination and inactivating the nf-κb signaling pathway.Graphical abstract

Journal: Arthritis Research and Therapy (2020)
Publication types: Research Article;
Jilin Province’s All-for-one Tourism Based on Artificial Intelligence and Synergy Theory

In modern society, the all-for-one economy is booming, and the economic level of the general public is also improving.After people have met their material needs, their spiritual needs are constantly increasing.Therefore, the tourism industry has achieved good development.As one of the important branches of system science, synergy theory is a discipline that studies the mechanism and law of orderly structure formed by open systems through the synergy between internal subsystems.It is the theoretical basis for constructing various systems and a method to solve complex system problems.Introducing synergy theory into all-for-one tourism research and studying all-for-one tourism development issues from a systematic point of view is of practical significance for solving various comprehensive and complex issues that have always occurred in the development of tourism.As the current hotspot of high-tech and new technology development, artificial intelligence technology has gradually been widely used in all aspects of social production and life, especially for the development and promotion of all-for-one tourism.Therefore, in the development of the global domain, increasing the application of artificial intelligence technology also has positive practical significance.Artificial intelligence technology can promote the development of all-for-one tourism to improve quality and efficiency, and bring tourists a brand new travel experience.This article discusses the application of artificial intelligence and synergy theory in the all-for-one tourism of jilin province, and explores a scientific, effective and sustainable tourism model.The experimental results show that all-for-one tourism with the introduction of artificial intelligence and synergy theory is more in line with the current pace of life and living habits of young people, which is bound to be positive and beneficial to promote the development of all-for-one tourism in jilin province.

Journal: Journal of Physics: Conference Series (2021)
Publication types: Conference Abstract/Paper;
A Study on the Satisfaction of Practice and the willingness to Stay in the Post of Students in Tourism Management Major Based on Big Data Analysis

Through big data analysis, the satisfaction of students majoring in tourism management in practice was research and observe.A questionnaire was compiled to investigate the satisfaction and retention intentions of the tourism management major in internship.The results show that training opportunities, working environment, salary conditions, promotion space, employee relations, professional respect and other factors affect the internship satisfaction of tourism management students.Based on the results of the questionnaire survey, this paper proposes corresponding solutions.

Journal: Journal of Physics: Conference Series (2021)
Publication types: Conference Abstract/Paper;
Study on Hypoglycemic Effect of Mulberry Leaf Extract Based on Big Data analysis

With the improvement of people’s living standards and the emergence of aging society, the incidence of diabetes is increasing year by year, and diabetes and its complications have become the third killer threatening human health.There are many drugs for treating diabetes, but their toxic and side effects are all great.Therefore, using big data analysis to find and develop natural products with hypoglycemic effect has high practical value.Therefore, the purpose of this paper is to study the hypoglycemic effect of mulberry leaf extract based on big data analysis.Firstly, this paper studies the medicinal value of mulberry leaves and related application methods by consulting relevant data.Through big data analysis, it is found that mulberry leaves are rich in alkaloid component 1-deoxynojirimycin (dnj), accounting for more than 50% of alkaloid content.Therefore, this paper takes mulberry leaf extract dnj as the research object, and firstly prepares mulberry leaf extract.Mulberry leaf water extract was perfused into stomach for 15 consecutive days, and fasting blood glucose of diabetic mice was measured 2 hours after eating by glucose oxidase method at specified time.The hypoglycemic effect of mulberry leaf water extract was analyzed by big data.The results showed that mulberry leaf extract could effectively inhibit the enzyme activity of decomposing sugar and prevent glucose absorption.Therefore, it can significantly reduce the blood sugar level of diabetic mice by about 50%.And the higher the dose of mulberry leaf extract, the better the hypoglycemic effect, showing a dose effect.

Journal: Journal of Physics: Conference Series (2021)
Publication types: Conference Abstract/Paper;