syedzayyan/paper_mill_benchmark huggingface dataset:
KLHDC8A Expression in Association with Macrophage Infiltration and Oxidative Stress Predicts Unfavorable Prognosis for Glioma

The tumor immune microenvironment (tme) is associated with cancer progression and immune escape.Although klhdc8a has been reported in glioma in vitro, the expression and clinical significance of this gene in clinical samples are unknown.Methods.The cancer genome atlas and chinese glioma genome atlas databases were used to evaluate the m rna expression level of klhdc8a and its significance in the glioma tme.Tissue microarray-based multiple immunohistochemical staining was conducted to determine klhdc8a protein levels and characterize the immune signature of tumor-infiltrating immune cells in gliomas.Results.Tumor cells and tumor-associated macrophages expressed klhdc8a.The expression of klhdc8a was higher in glioma tissues than in normal brain tissues and was associated with patient clinical characteristics.Gliomas exhibited a high abundance of macrophages, neutrophils, regulatory t cells, and the immune checkpoint pd-l1, as well as high klhdc8a expression.Cox regression analysis showed that klhdc8a+cd68+ macrophages and klhdc8a predicted unfavorable survival in patients with glioma.Finally, protein-protein interaction network analysis showed that the klhdc8a expression was associated with hypoxia and oxidative stress.Conclusions.Klhdc8a is a potential marker for the clinical diagnosis of glioma.The immune characteristics of macrophages play a crucial role in predicting patients with glioma, providing a new avenue for targeted glioma therapy.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Investigation of the Effects of Large Bone Flap Craniotomy on Cerebral Hemodynamics, Intracranial Infection Rate, and Nerve Function in Patients with Severe Craniocerebral Trauma

In order to explore the clinical value of large bone flap craniotomy, the effects of standard large bone flap craniotomy on cerebral hemodynamic indexes, incidence of postoperative intracranial infection, and neurological function in patients with severe craniocerebral trauma are investigated.89 patients with severe craniocerebral trauma admitted from january 2020 to june 2021 are analyzed retrospectively.All patients are divided into a large craniotomy group ( n = 45) and control group ( n = 44) according to different surgical methods.The large craniotomy group is treated with large craniotomy decompression, and the control group is treated with traditional craniotomy decompression.The incidence of intracranial infection in each group is recorded, and nihss is applied to observe the neurological function recovery of 2 groups before and 1 month after operation.Besides, the patients are followed up after surgery and the kaplan–meier survival curve is obtained to compare the survival rate of patients in the two groups.It is clearly evident that the two surgical methods have certain clinical efficacy in the treatment of patients with severe craniocerebral trauma.Comparatively, the large craniotomy can further improve brain blood supply and improve neurological function recovery.Also, it can obtain low incidence of postoperative adverse reactions and intracranial infection.

Journal: Contrast Media & Molecular Imaging (2022)
Publication types: Research Article;
Investigation of the Clinical Application Value of HR-HPV DNA Combined with Liquid Based Cytology in Colposcopy of Cervical Cancer

In order to investigate the clinical application value of hr‐hpv dna combined with liquid‐based cytology in colposcopy of cervical cancer, a retrospective analysis is performed on 428 patients who underwent a cervical pathological examination in our hospital from may 2020 to december 2021.The pathological biopsy results are used as the gold standard to determine whether cervical lesions occurred in patients, and patients with positive gold standard results are included in the study group.The positive rates of liquid‐based cytology and hr‐hpv dna are observed and recorded, and the patients with negative gold standard results are used as the control group.The positive rate of hr‐hpv dna and liquid‐based cytology will increase with the aggravation of pathological results.The roc curve shows that hr‐hpv dna combined with liquid‐based cytology has high diagnostic efficiency in colposcopy of cervical lesions.It is clearly evident that both liquid‐based cytology and hr‐hpv dna tests have certain advantages in the screening of cervical lesions, and the combined detection can further improve the screening value of cervical lesions.

Journal: Contrast Media & Molecular Imaging (2022)
Publication types: Research Article;
Inhibition of TLR4 Suppresses the Inflammatory Response in Inflammatory Bowel Disease (IBD) by Modulating the PDK1-Induced Metabolism Reprogramming via a m6A-Denpendent Manner

To investigate the role of tlr4 and pdk1 genes in ibd.Methods.The dss mouse model was established by inducing balb/c with 5% dss solution.The behavior of dss mice was detected, and the m6a modification was detected by m6a methylation chip.At the same time, the expressions of tlr and pdk1 were detected by fluorescence real-time quantitative pcr.Results.The results showed that the model of dextran sodium sulfate colitis in mice was successful, and the colon membrane of mice had obvious naked eye inflammation.Through comparison, it was found that there were differences in m6a modification between the blank group and the model group, and compared with the blank group, the expression of pkd1 in dss group was significantly reduced and the expression of tlr4 was significantly increased.Conclusion.Tlr4 inhibition inhibits the inflammatory response in inflammatory bowel disease (ibd) in a m6a-dependent manner by regulating pdk1-induced metabolic reprogramming.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
Influence of Adenoid Hypertrophy on Malocclusion and Maxillofacial Development in Children

To investigate the effect of adenoid hypertrophy on malocclusion and maxillofacial development in children.Methods.Total of 102 children with malocclusion or maxillofacial dysplasia admitted to our hospital from march 2017 to june 2020 were selected as the research subjects.All children were divided into a control group (50 cases with adenoid hypertrophy) and an observation group (52 cases without adenoid hypertrophy) according to the presence or absence of adenoid hypertrophy.The incidence of malocclusion was compared between the two groups, and lateral cranial radiographs were taken in both groups to measure and compare the malocclusion angle, jaw angle, and jaw length indexes between the two groups.Results.The incidence of malocclusion in the observation group (71.15%) was higher than that in the control group (42.00%), and the difference was statistically significant ( p &lt; 0.05 ).The angle between the long axis of the upper central incisor and the nasal root point and the upper alveolar base point (u1-na), the angle between the long axis of the lower central incisor and the nasal root point and the lower alveolar base point (l1-nb), the angle between mandibular plane and anterior cranial base plane (mp-sn), the angle between the long axis of upper central incisor and anterior cranial base plane (u1-sn), the angle between the long axis of lower central incisor and mandibular plane (l1-mp), the angle of y axis, the overall height (n-me), lower height (ans-me), overall height/back height (n-me/s-go), and lower height/overall height (ans-me/n-me) values in the observation group were higher than those in the control group, while the mandibular length (go-gn) values in the observation group were lower than those in the control group ( p &lt; 0.05 ).Conclusion.Adenoid hypertrophy can increase the incidence of malocclusion in children and can also increase the steepness and overall height and lower height of the mandible, resulting in the lengthening of the facial shape and the development of the maxillofacial deformity.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;
Impacts of Ultrasound-Guided Nerve Block Combined with General Anesthesia with Laryngeal Mask on the Patients with Lower Extremity Fractures

Surgical reduction is the leading approach to patients with lower extremity fractures.The options of anesthetic drugs during surgery are of great significance to postoperative recovery of patients.There is no consensus on the optimum anesthesia method for patients undergoing lower extremity fracture surgery.Our study is aimed at investigating the impacts of nerve block combined with general anesthesia on perioperative outcomes of the patients.Methods .In this retrospective study, 48 patients experienced general anesthesia only, and 42 patients received never block combined with general anesthesia.The perioperative hemodynamics was recorded, including mean arterial pressure (map), oxygen saturation of blood (sp o2), and heart rate (hr).Visual analogue scale (vas) and montreal cognitive assessment (mo ca) were carried out to evaluate postoperative pain and cognitive status.Furthermore, adverse reactions and recovery condition were observed between the patients receiving different anesthesia methods.Results .At 15 minutes and 30 minutes after anesthesia, as well as 5 minutes after surgery, significant lower map was observed in the patients treated with general anesthesia (83.04 ± 8.661, 79.17 ± 9.427, 86.58 ± 8.913) compared to those receiving never block combined with general anesthesia (90.43 ± 4.618, 88.74 ± 6.224, 92.21 ± 4.015) ( p &lt; 0.05), and compared with general anesthesia group (68.5 ± 7.05, 69.63 ± 7.956, 72.75 ± 8.446), the combined anesthesia group (73.52 ± 9.451, 74.17 ± 10.13, 77.62 ± 9.768) showed obvious higher hr ( p &lt; 0.05).No significant difference in sp o 2 was found between the two groups at multiple time points ( p &gt; 0.05).As for the score of vas and mo ca, remarkably lower vas and higher mo ca at 6 h, 12 h and 24 h after surgery were presented in the combined anesthesia group compared to general anesthesia group ( p &lt; 0.05).At 24 h after surgery, the two groups showed normal cognitive function (26.33 ± 0.7244 vs.28.55 ± 0.7392).Incidence of nausea and vomiting in the combined anesthesia group was lower than that of the general anesthesia group ( p &lt; 0.05).The time to out‐of‐bed activity and hospital stay were shorter in the combined anesthesia group compared with general anesthesia ( p &lt; 0.05).Conclusion .The application of never block combined with general anesthesia contributed to the stability of hemodynamics, alleviation of postoperative pain and cognitive impairment, along with decrease in adverse reactions and hospital stay in the patients with lower extremity fractures.

Journal: Journal of Environmental and Public Health (2022)
Publication types: Research Article;
Lactobacillus helveticus Prevents Periodontitis Induced by Aggregatibacter actinomycetemcomitans in Rats by Regulating β-Defensins

To study the preventive effect of lactobacillus helveticus (l.Helveticus) on periodontitis induced by aggregatibacter actinomycetemcomitans (a.Actinomycetemcomitans) in rats.Methods.Eighteen 8-week-old female rats were randomly divided into three groups: sham group, trehalose group, and l.Helveticus sbt2171 (lh2171) group.We measured the distance of the cementoenamel junction-alveolar bone crest (cej-abc) to evaluate alveolar bone resorption.Hematoxylin-eosin staining was used to observe the histopathological changes of rat hemimaxillary tissues.We detected the expression of β-defensins, tumor necrosis factor-α (tnf-α), interleukin- (il-) 1β, and il-6 and the number of a.Actinomycetemcomitans in rat gingival tissues by quantitative reverse transcriptase polymerase chain reaction.The levels of il-1β, il-6, and tnf-α in rat gingival tissues were also measured by enzyme-linked immunosorbent assay.Results.Compared with the trehalose group, the distance of cej-abc was prominently reduced and alveolar bone resorption was notably improved in the lh2171 group.And the infiltration of inflammatory cells in the hemimaxillary tissue decreased obviously, periodontal fibers were arranged neatly, connective tissue small blood vessels proliferated, and the number of a.Actinomycetemcomitans reduced significantly in the lh2171 group.In addition, the m rna expression and release of inflammatory factors in the gingival tissues in the lh2171 group were notably lower than those in the trehalose group.On the 21st and 36th day, the expression of β-defensins in the gingival tissue of the lh2171 group increased significantly.Conclusion.L.Helveticus improves alveolar bone resorption and increases the expression of β-defensins thereby inhibiting the number of a.Actinomycetemcomitans and thus prevents periodontitis.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
Influence of Case Management Model Combined with Continuous Nursing Care on Compliance Behavior and Adverse Emotions in Elderly Patients with Lung Cancer: A Prospective Single-Center Case-Control Study

S.To ask lots of questions about finding the truth about the influence of the case management model combined with continuous nursing care on following the law behavior and negative feelings of love, hate, fear, etc.In old patients with lung scale-like cell cancer.Materials and methods.One hundred and forty-three elderly patients with squamous cell carcinoma of the lung were selected for this prospective study, 10 cases were shed due to epidemic and transfer, and finally 68 cases were in the control group and 65 cases in the observation group.The differences in anxiety and depression scores, quality of life, and compliance behavior between the two groups were observed and compared.Results.After nursing, the self-rating anxiety scale (sas) and self-rating depression scale (sds) of the observation group were lower than those of the control group, while the social support score was significantly higher than that of the control group.The scores of psychological behavior, exercise status, drug taking, and balanced diet of the two groups were significantly improved, and the observation group was significantly improved.The scores of medical compliance behavior in the observation group were significantly higher than those in the control group, and the mental vitality score, social interaction score, emotional restriction score, and mental status of the patients in the observation group were significantly higher than those in the control group, and the above statistics showed that the difference was statistically significant ( p &lt; 0.05 ).Conclusion.The use of a case management model combined with extended care significantly improved the compliance behavior and anxiety and depression of elderly patients with squamous cell carcinoma of the lung and improved the quality of life and social support.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Clinical Study;
Perception Research of Artificial Intelligence in Environmental Public Health Physiotherapy Nursing for the Elderly

This perceptual study focuses on developing artificial intelligence for elderly care design.It analyses and discusses the role of artificial intelligence in elderly care and its application to physiotherapy care.Artificial intelligence, as an emerging disruptive technology, is releasing the enormous energy accumulated in the technological and industrial revolutions, profoundly transforming how humans produce, live, and think about the world.Economic development and social progress are significantly impacted by it, and it has a great deal of practicality and broad application scope.Although there is a basic consensus in the 18 public understanding of ai, there is still some ambiguity and misunderstanding, knowing what is happening without understanding why.As a result, it is necessary to systematize and gain a comprehensive understanding of this concept and its associated practices.

Journal: Journal of Environmental and Public Health (2022)
Publication types: Research Article;
In Vitro Evaluation of Cytotoxic Potential of Caladium lindenii Extracts on Human Hepatocarcinoma HepG2 and Normal HEK293T Cell Lines

Data regarding the therapeutic potential of caladium lindenii ( c.Lindenii ) are insufficient.It becomes more important to explore plants as an alternative or palliative therapeutics in deadly diseases around the globe.The current study was planned to explore c.Lindenii for its anticancer activity of ethanolic and hexane extracts of c.Lindenii leaves against hepatic carcinoma (hep g2) and human embryonic kidney (hek293t) cell lines.Hep g2 and hek293t cells were treated with 10, 50, 100, 200, and 400 μ g/m l of ethanolic and hexane extracts of c.Lindenii and were incubated for 72 h.Antiproliferative activity was measured by 3‐(4,5‐dimethylthiazol‐2yl)‐2,5‐biphenyl tetrazolium bromide (mtt) assay, and percentage viability were calculated through crystal violet staining and cellular morphology by floid cell imaging station.The study showed ethanolic extract exhibiting a significantly higher antiproliferative effect on hep g2 (ic50 = 31 μ g/m l) in a concentration‐dependent manner, while hek293t (ic50 = 241 μ g/m l) cells showed no toxicity.Hexane extract exhibited lower cytotoxicity (ic50 = 150 μ g/m l) on hep g2 cells with no effect on hek293t (ic50 = 550 μ g/m l).On the other hand, the percentage viability of hep g2 cells was recorded as 78%, 67%, 50%, 37%, and 28% by ethanolic extracts, and 88%, 80%, 69%, 59%, and 50% by hexane extracts at tested concentrations of both extracts.Toxicity assay showed significantly safer ranges of percentage viabilities in normal cells (hek293t), i.e., 95%, 90%, 88%, 76%, and 61% with ethanolic extract and 97%, 95%, 88%, 75%, and 62% with hexane extract.The assay validity revealed 100% viability in the control negative (dimethyl sulfoxide treated) and less than 45% in the control positive (cisplatin) on both hep g2 and hek293t cells.Morphological studies showed alterations in hep g2 cells upon exposure to &gt;50 μ g/m l of ethanolic extracts and ≥400 μ g/m l of hexane extracts.Hek293t on the other hand did not change its morphology against any of the extracts compared to the aggressive changes on the hep g2 cell line by both extracts and positive control (cisplatin).In conclusion, extracts of c.Lindenii are proved to have significant potential for cytotoxicity‐induced apoptosis in human cancer hep g2 cells and are less toxic to normal hek293t cells.Hence c.Lindenii extracts are proposed to be used against hepatocellular carcinoma (hcc) after further validations.

Journal: BioMed Research International (2022)
Publication types: Research Article;
DNA Topoisomerase II-α Regulated by miR-22-5p Promotes Hepatocellular Carcinoma Invasion and Migration through the Hippo Pathway

Dna topoisomerases (tops) are dysregulated in various types of cancer.However, how top ii-alpha (top2a) contributes to hepatocellular carcinoma (hcc) progression remains elusive.Cohort analysis revealed that the increased expression of top2a was associated with poor clinical outcomes and top2a was significantly upregulated in hcc tissues and cell lines.In vitro, top2a expression level is related to cell invasion and migration, which may be due to the alteration of epithelial-mesenchymal transition by the top2a.Moreover, we used verteporfin (a hippo inhibitor) to test how the hippo pathway promotes the effect of top2a on the hcc phenotype and found that top2a induces tumor progression through the hippo pathway.Finally, mi r-22-5p inhibited tumor progression by sponging top2a.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Identification of the HMMR Gene as a Diagnostic and Prognostic Biomarker in Hepatocellular Carcinoma Based on Integrated Bioinformatics Analysis

We aimed to investigate the expression of the hyaluronan-mediated motility receptor (hmmr) gene in hepatocellular carcinoma (hcc) and nonneoplastic tissues and to investigate the diagnostic and prognostic value of hmmr.Method.With the reuse of the publicly available the cancer genome atlas (tcga) data, 374 hcc patients and 50 nonneoplastic tissues were used to investigate the diagnostic and prognostic values of hmmr genes by receiver operating characteristic (roc) curve analysis and survival analysis.All patients were divided into low- and high-expression groups based on the median value of hmmr expression level.Univariate and multivariate cox regression analysis were used to identify prognostic factors.Gene set enrichment analysis (gsea) was performed to explore the potential mechanism of the hmmr genes involved in hcc.The diagnostic and prognostic values were further validated in an external cohort from the international cancer genome consortium (icgc).Results.Hmmr m rna expression was significantly elevated in hcc tissues compared with that in normal tissues from both tcga and the icgc cohorts (all p values &lt;0.001).Increased hmmr expression was significantly associated with histologic grade, pathological stage, and survival status (all p values &lt;0.05).The area under the roc curve for hmmr expression in hcc and normal tissues was 0.969 (95% ci: 0.948–0.983) in the tcga cohort and 0.956 (95% ci: 0.932–0.973) in the icgc cohort.Patients with high hmmr expression had a poor prognosis than patients with low expression group in both cohorts (all p &lt; 0.001 ).Univariate and multivariate analysis also showed that hmmr is an independent predictor factor associated with overall survival in both cohorts (all p values &lt;0.001).Gsea showed that genes upregulated in the high-hmmr hcc subgroup were mainly significantly enriched in the cell cycle pathway, pathways in cancer, and p53 signaling pathway.Conclusion.Hmmr is expressed at high levels in hcc.Hmmr overexpression may be an unfavorable prognostic factor for hcc.

Journal: Evidence-Based Complementary and Alternative Medicine (2021)
Publication types: Research Article;
HMGB3 Targeted by miR-145-5p Impacts Proliferation, Migration, Invasion, and Apoptosis of Breast Cancer Cells

This study focused on the investigation into how hmgb3 works in breast cancer (bc) progression.Firstly, we analyzed the relationship between hmgb3 and bc patients through the tcga database.We performed q rt-pcr for determining the hmgb3 m rna level and western blot for detecting the protein level of hmgb3 in bc cell lines.Cck-8, flow cytometry, transwell, and wound healing assays were utilized to detect the effect of hmgb3 on bc cell phenotypes.Next, the prediction of the binding site shared by mi r-145-5p and hmgb3 was performed by the bioinformatics method.The targeting relationship between mi r-145-5p and hmgb3 was validated by using dual-luciferase assay.Finally, rescue experiments were employed for assessing the effect of the mi r-145-5p/hmgb3 axis on bc cells.Hmgb3 was demonstrated to have a high-level expression in bc cell lines and facilitated bc progression.On the contrary, mi r-145-5p was shown a low-level expression in bc cell lines, which could target hmgb3.Mi r-145-5p restrained the proliferation, migration, and invasion of bc cells via inhibiting hmgb3.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;