syedzayyan/paper_mill_benchmark huggingface dataset:
Rutin Promotes Pancreatic Cancer Cell Apoptosis by Upregulating miRNA-877-3p Expression.

(1) background: pancreatic cancer is one of the most serious cancers due to its rapid and inevitable fatality, which has been proved very difficult to treat, compared with many other common cancers.Thus, developing an effective therapeutic strategy, especially searching for potential drugs, is the focus of current research.The exact mechanism of rutin in pancreatic cancer remains unknown.(2) method: three pancreatic cancer cell lines were used to study the anti-pancreatic cancer effect of rutin.The potent anti-proliferative, anti-migration and pro-apoptotic properties of rutin were uncovered by cell viability, a wound-healing migration assay, and a cell apoptosis assay.High-throughput sequencing technology was used to detect the change of mi rnas expression.Immunoblotting analysis was used to detect the expression of apoptotic proteins.(3) results: cck-8 and edu assays revealed that rutin significantly inhibited pancreatic cancer cells’ proliferation (p < 0.05).A wound-healing assay showed that rutin significantly suppressed pancreatic cancer cells’ migration (p < 0.05).A flow cytometric assay showed that rutin could promote pancreatic cancer cells’ apoptosis.Intriguingly, rutin significantly upregulated mi r-877-3p expression to repress the transcription of bcl-2 and to induce pancreatic cancer cell apoptosis.Accordingly, rutin and mi r-877-3p mimics could promote apoptotic protein expression.(4) conclusions: our findings indicate that rutin plays an important role in anti-pancreatic cancer effects through a rutin-mi r-877-3p-bcl-2 axis and suggests a potential therapeutic strategy for pancreatic cancer.

Journal: Molecules (Basel, Switzerland) (2022)
Publication types: Journal Article
Effects of Quercetin on the Intestinal Microflora of Freshwater Dark SleeperOdontobutis potamophila.

Flavonoids have antimicrobial and anti-oxidation properties.The effects of the flavonoid quercetin on the intestinal microflora of freshwater dark sleeper odontobutis potamophilawere tested for the first time.odontobutis potamophilajuveniles were treated with quercetin for 21 days at one of three concentrations (2.5, 5.0, or 10.0 mg/l) and compared with a control group that was not treated with quercetin.Quercetin improved the stability of the intestinal flora in o.Potamophilaand the probiotic bacteria bacillusspp.And lactobacillusspp.Increased in species abundance after the low concentration quercetin treatments.Furthermore, the abundance of pathogenic bacteria plesiomonasspp.,aeromonasspp., and shewanellaspp.Decreased after the fish had been exposed to quercetin.Activity of hepatic antioxidant enzymes (superoxide dismutase, sod), (glutathione s-transferase, gst), (glutathione peroxidase, gsh-px), and (total antioxidant capacity, t-aoc) increased in the livers of o.Potamophilatreated with quercetin, thereby increasing their hepatic antioxidant capacity and their ability to scavenge free radicals.

Journal: Antioxidants (Basel, Switzerland) (2022)
Publication types: Journal Article
Clinical management of hip fractures in elderly patients with dementia and postoperative 30-day mortality: A population-based cohort study.

S patients with dementia have an increased 30-day mortality after hip fracture.We investigated clinical management including time to surgery, out-of-hours admission and surgery, surgery on weekends, surgery volume per ward, and anesthesia technique for this excess mortality risk.Method this register- and population-based study comprised 12,309 older adults (age 70+) admitted to hospital for a first-time hip fracture in 2013-2014, of whom 11,318 underwent hip fracture surgery.Cox proportional hazards regression models were applied for the analysis.Results the overall postoperative 30-day mortality was 11.4%.Patients with dementia had a 1.5 times increased mortality risk than those without (hr = 1.50 [95% ci 1.31-1.72]).We observed no time-to-surgery difference by patient dementia status; additionally, the excess mortality risk in patients with dementia could not be explained by the clinical management factors we examined.Conclusions increased mortality in patients with dementia could not be explained by the measured preoperative clinical management.Suboptimal handling of postoperative complication and rehabilitation are to be investigated for their role in the witnessed increased mortality for patients with dementia.

Journal: Brain and behavior (2020)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Novel Nano-Drug Delivery System for Brain Tumor Treatment.

As the most dangerous tumors, brain tumors are usually treated with surgical removal, radiation therapy, and chemotherapy.However, due to the aggressive growth of gliomas and their resistance to conventional chemoradiotherapy, it is difficult to cure brain tumors by conventional means.In addition, the higher dose requirement of chemotherapeutic drugs caused by the blood-brain barrier (bbb) and the untargeted nature of the drug inevitably leads to low efficacy and systemic toxicity of chemotherapy.In recent years, nanodrug carriers have attracted extensive attention because of their superior drug transport capacity and easy-to-control properties.This review systematically summarizes the major strategies of novel nano-drug delivery systems for the treatment of brain tumors in recent years that cross the bbb and enhance brain targeting, and compares the advantages and disadvantages of several strategies.

Journal: Cells (2022)
Publication types: Journal Article;Review
Immunomodulation as a Potent COVID-19 Pharmacotherapy: Past, Present and Future.

In the first year of its appearance, the 2019 coronavirus disease (covid-19) has affected more than 150 million individuals and killed 3 million people worldwide.The pandemic has also triggered numerous global initiatives to tackle the newly emerging disease, including the development of sars-co v-2 vaccines and the attempt to discover potential pharmacological therapies.Nonetheless, despite the success of sars-co v-2 vaccine development, covid-19 therapy remains challenging.Several repurposed drugs that were documented to be useful in small clinical trials have been shown to be ineffective in larger studies.Additionally, the pathophysiology of sars-co v-2 infection displayed the predominance of hyperinflammation and immune dysregulation in inducing multiorgan damage.Therefore, the potential benefits of both immune modulation and suppression in covid-19 have been extensively discussed.Here, we reviewed the roles of immunomodulation as potential covid-19 pharmacological modalities based on the existing data and proposed several new immunologic targets to be tested in the foreseeable future.

Journal: Journal of inflammation research (2021)
Publication types: Journal Article;Review
Genetically Decreased Circulating Vascular Endothelial Growth Factor and Osteoporosis Outcomes: A Mendelian Randomization Study.

Vascular endothelial growth factor (vegf) is important for bone formation and has been associated with osteoporosis in humans.Therefore, we conducted a two-sample mendelian randomization study to test whether genetically decreased circulating vegf was associated with decreased bone mineral density (bmd) and increased risk of fracture.Summary statistics from a genomewide association study (gwas) meta-analysis of circulating vegf level (n = 16,112) were used to identify 10 genetic variants explaining up to 52% of the variance in circulating vegf levels.Gwas meta-analyses on dual-energy x-ray absorptiometry (dxa)-derived bmd of forearm, lumbar spine, and femoral neck (n = up to 32,735) and bmd estimated from heel calcaneus ultrasound (e bmd) (n = 426,824) were used to assess the effect of genetically lowered circulating vegf levels on bmd.A gwas meta-analysis including a total of 76,549 cases and 470,164 controls was used to assess the effect of genetically lowered circulating vegf levels on risk of fracture.A natural log-transformed pg/m l decrease in circulating vegf levels was not associated with a decrease in forearm bmd (0.02 standard deviation [sd], 95% confidence interval [ci] -0.024 to 0.064, p = 0.38), lumbar spine bmd (-0.005 sd, 95% ci -0.03 to 0.019, p = 0.67), femoral neck bmd (0.004 sd, 95% ci -0.017 to 0.026, p = 0.68), e bmd (-0.006 sd, 95% ci -0.012 to -0.001, p = 0.031) or risk of fracture (odds ratio = 0.99, 95% ci 0.98 to 1.0, p = 0.37) in inverse-variance-weighted mendelian randomization analyses.Sensitivity analyses did not provide evidence that our results were influenced by pleiotropy.Genetically lowered circulating vegf was not associated with a decrease in bmd or increased risk of fracture, suggesting that efforts to influence circulating vegf level are unlikely to have beneficial effects on osteoporosis outcomes and that previous observational associations of circulating vegf with bmd were influenced by confounding or reverse causation.© 2019 american society for bone and mineral research.

Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research (2020)
Publication types: Journal Article;Meta-Analysis;Research Support, Non-U.S. Gov't
Converted laparoscopic distal pancreatectomy: is there an impact on patient outcome and total cost?

Recent studies have reported worse outcomes of converted laparoscopic distal pancreatectomy (cldp) with respect to total laparoscopic (tldp) and open (odp).The aim of the study was to evaluate the impact of conversion on patient outcome and on total cost.Methods patients requiring a conversion (cldp) were compared with both tldp and odp patients.The relevant patient- and tumour-related variables were collected for each patient.Both intra and postoperative data were extracted.Propensity score matching (psm) analysis was carried out to equate the groups compared.Results two hundred and five patients underwent dp, 105 (51.2%) odps, 81 (39.5%) tldps, and 19 (9.3%) cldps.After psm, 19 cldps, 38 tldps, and 38 odps were compared.Patients who underwent cldp showed a significantly longer operative time (p < 0.001), and an increase in blood loss (p = 0.032) and total cost (p = 0.034) with respect to tldp, and a significantly longer operative time (p < 0.001), less frequent postoperative morbidity (p = 0.050), and a higher readmission rate (p = 0.035) with respect to odp.Conclusion total laparoscopic pancreatectomy was superior regarding operative findings and total costs with respect to cldp; odp showed a higher postoperative morbidity rate and a lower readmission rate with respect to cldp.However, the reasons for the readmission of patients who underwent cldp were mainly related to postoperative pancreatic fistula (popf) grade b which is usually due to pancreas texture.Thus, the majority of distal pancreatectomies can be started using a minimally invasive approach, performing an early conversion if necessary.

Journal: Langenbeck's archives of surgery (2022)
Publication types: Journal Article
The Challenges of Enrollment and Retention: A Systematic Review of Psychosocial Behavioral Interventions for Patients With Cancer and Their Family Caregivers.

Context psychosocial behavioral interventions (pbis) that target patients with cancer and their caregivers face challenges in participant enrollment and retention.Objectives 1) describe characteristics of the patient-caregiver pbi studies; 2) examine participant enrollment and retention rates; 3) identify factors influencing participant enrollment and retention rates; and 4) explore the strategies to promote enrollment and retention rates.Methods we identified randomized controlled trials that tested pbis among adult patients with cancer and caregivers in five electronic databases.We conducted narrative and quantitative analyses to synthesize our findings.Results among 55 qualified studies reviewed, most tested the efficacy of pbis (n = 42) and used two study arms (n = 48).In-person meeting was the most common pbi delivery mode.The primary outcomes included quality of life, physical health, and symptoms.The average of enrollment rates of patient-caregiver dyads was 33% across studies (range 8%-100%; median = 23%).The average retention rate at the end of follow-ups was 69% (range 16%-100%; median = 70%).The number of study arms, recruitment method, type of patient-caregiver relationship, and intervention duration influenced enrollment rates.Study design (efficacy vs.Pilot), follow-up duration, mode of delivery, type of relationship, and intervention duration influenced retention rates.Sixteen studies reported retention strategies, including providing money/gift cards upon study completion and/or after follow-up survey, and excluding patients with advanced cancer.Conclusion researchers need to incorporate effective strategies to optimize enrollment and retention in patient-caregiver pbi trials.Researchers need to report detailed study processes and pbi information to improve research transparency and increase consistency.

Journal: Journal of pain and symptom management (2021)
Publication types: Journal Article;Research Support, N.I.H., Extramural;Research Support, Non-U.S. Gov't;Systematic Review
Unfolding the Complexity of the Global Value Chain: Strength and Entropy in the Single-Layer, Multiplex, and Multi-Layer International Trade Networks.

The worldwide trade network has been widely studied through different data sets and network representations with a view to better understanding interactions among countries and products.Here we investigate international trade through the lenses of the single-layer, multiplex, and multi-layer networks.We discuss differences among the three network frameworks in terms of their relative advantages in capturing salient topological features of trade.We draw on the world input-output database to build the three networks.We then uncover sources of heterogeneity in the way strength is allocated among countries and transactions by computing the strength distribution and entropy in each network.Additionally, we trace how entropy evolved, and show how the observed peaks can be associated with the onset of the global economic downturn.Findings suggest how more complex representations of trade, such as the multi-layer network, enable us to disambiguate the distinct roles of intra- and cross-industry transactions in driving the evolution of entropy at a more aggregate level.We discuss our results and the implications of our comparative analysis of networks for research on international trade and other empirical domains across the natural and social sciences.

Journal: Entropy (Basel, Switzerland) (2018)
Publication types: Journal Article
Treatment of ibuprofen intoxication with charcoal haemoperfusion in two dogs.

Case history:two dogs presented separately to the small animal hospital, university of florida (gainsville, fl, usa) for ingestion of ibuprofen.The first dog ingested 561.8 mg/kg ibuprofen in addition to paracetamol and caffeine and vomited prior to admission.This patient also received fluid therapy for 8 hours prior to charcoal haemoperfusion.The second dog ingested 500 mg/kg of ibuprofen and the owners induced vomiting with hydrogen peroxide prior to presentation.Due to the severity of clinical signs, both patients were treated with charcoal haemoperfusion.clinical findings:the concentrations of ibuprofen in the blood of the dogs prior to treatment were 478 and 301 mg/l.During the treatment ibuprofen concentrations were reduced by 95.8% and 45.5%, respectively, with no treatment side effects and minimal clinical signs after treatment.diagnosis:toxicity due to ingestion of ibuprofen toxicity that was successfully treated with charcoal haemoperfusion.clinical relevance:in the cases described here minimal benefit was seen after 3 hours of treatment using one haemoperfusion cartridge.This is in contrast to a previously published report in which dogs were treated for 6 hours with two charcoal haemoperfusion cartridges.This suggests that one cartridge may be sufficient.The amount of ibuprofen ingested was not a reliable predictor of the concentration in blood at the initiation of treatment.Charcoal haemoperfusion is an effective means of reducing plasma concentrations of ibuprofen, however, its use may be limited by its cost and availability.

Journal: New Zealand veterinary journal (2020)
Publication types: Case Reports;Journal Article
Modulatory effect of IL-1 inhibition following lipopolysaccharide-induced neuroinflammation in neonatal microglia and astrocytes.

Introduction inflammation-induced white matter injury (wmi) in preterm infants is characterized by microglia activation, astrogliosis, oxidative stress and neurodevelopmental impairments.Microglia and astrocytes activation can be described under a broad spectrum of activation profile with extremes described as pro-inflammatory/neurotoxic (m1 microglia or a1 astrocyte) or anti-inflammatory/neuroprotective (m2 microglia or a2 astrocyte) in response to stimuli including lipopolysaccharide (lps) and interleukin 1 (il-1).As il-1 signalling pathway has been posited as a major driver of inflammation-induced perinatal wmi, our aim was to evaluate the contribution of il-1 modulation in lps-induced microglia and astrocyte activation.Methods primary neonatal cell co-cultures of astrocytes and microglia were treated with lps (100 ng/ml) for 8 h or 24 h.Two distinct il-1 receptor antagonists, rytvela or kineret (1 μg/ml), were added simultaneously with lps to respectively modulate or block il-1 receptor.Medium was collected to measure levels of il-1β.Expression of markers related to pro- and anti-inflammatory microglia and astrocyte activation profiles and antioxidant genes were assessed.Results at 8 h, lps exposure induced pro- (m1/a1) and anti-inflammatory (m2/a2) marker expression and inhibited antioxidant gene expression in microglia and astrocytes.By 24 h, continuous lps exposure increased pro-inflammatory and neurotoxic microglial and astrocytic markers (m1/a1), as well as antioxidant genes.Administration of il-1 antagonists rytvela and kineret with continuous lps exposure had no significant effect on modulation of specific microglia and astrocyte activation pathways.Discussion/conclusion we show that lps effects on in vitro neonatal microglia and astrocytes co-cultures are time dependent eliciting a number of pro- and anti-inflammatory responses during the acute phase of inflammation (8 h), which shift towards pro-inflammatory and neurotoxic factors by 24 h.Although lps-induced inflammation led to abundant il-1 expression, il-1 inhibition had no significant impact on in vitro modulation of microglia and astrocyte activation pathways towards m2 and a2 activation profile.

Journal: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience (2022)
Publication types: Journal Article
Axillary Lymph Node Tattooing and Targeted Axillary Dissection in Breast Cancer Patients Who Presented as cN+ Before Neoadjuvant Chemotherapy and Became cN0 After Treatment.

Introduction targeted axillary dissection (tad) is an alternative to axillary dissection for breast cancer patients who presented as c n+ before neoadjuvant chemotherapy (nac) and became c n0 after treatment.Tad is defined as the removal of sentinel lymph nodes (slns) along with the pre-nac marked positive nodes.Tattooing is an option to mark positive nodes.In this study we aimed to investigate the identification rate of tattooed nodes during surgery, correspondence between tattooed nodes and slns, and difficulties and pitfalls of the method.Patients and methods in 75 patients who were c n+, with axillary lymph nodes known to have or suspected to have disease were tattooed pre-nac with a sterile carbon suspension (spot).After nac completion all patients became c n0 and underwent tad as an axillary staging procedure.Results slns were identified successfully in 70 of 75 patients (93.3%).All tattooed nodes were identified successfully intraoperatively in 71 of 75 patients (94.6%).Retrieval of all tattooed nodes in surgical specimens was achieved in 74 patients (98.6%).Correspondence between tattooed nodes and slns was observed in 53 of 70 patients (75.3%).In 34 patients (45.3%) the number of pigmented nodes in pathological examination was greater than the number of initially tattooed nodes, indicating the possibility of tattoo ink migration.Conclusion tattoo of axillary lymph nodes is a feasible, accurate, and low-cost method of positive node marking pre-nac.Pathological confirmation of black pigment in the lymph nodes excised is not by itself warranty of retrieval of all marked node because of tattoo ink migration from one node to another.Intraoperative identification using visual inspection is essential.

Journal: Clinical breast cancer (2019)
Publication types: Journal Article
The Impact of Biologic Drugs on High-Density Lipoprotein Cholesterol Efflux Capacity in Rheumatoid Arthritis Patients.

One of the most intriguing conundrums in patients with rheumatoid arthritis (ra) is the lack of correlation between cholesterol levels and cardiovascular (cv) events, diminishing the reliability of plasmatic lipid levels in estimating the cv risk.High-density lipoprotein cholesterol efflux capacity (hdlc-ec) directly indicates the functional ability of hdl to scavenge cholesterol from vascular wall and may provide better information on the atherogenic risk.The aim of this study was to examine the effects of different disease-modifying antirheumatic drugs on hdlc-ec in ra.Methods consecutive ra patients treated with different biologic disease-modifying antirheumatic drugs or methotrexate monotherapy were longitudinally observed.Demographic and clinical features as well as lipid profile were recorded at baseline, 24-week, and 52-week follow-up.At the same time points, hdlc-ec was evaluated using j771 macrophages and a fluorometric assay.Results we analyzed 100 ra patients on methotrexate, infliximab, tocilizumab, abatacept, or rituximab.No significant changes in the lipoprotein levels were detected, whereas the mean hdlc-ec statistically increased from baseline (22.5% ± 4.8%) to 24 weeks (24.5% ± 5.7%; p < 0.001) and 52 weeks (25.1% ± 5.9%; p < 0.001).Patients on tocilizumab showed the highest increase in hdlc-ec, already at 24 weeks.Patients on treatment with infliximab or rituximab showed a significant increase in hdlc-ec at 52 weeks.No significant changes were detected in abatacept and methotrexate groups.Conclusions some treatments may impact cholesterol reverse transport in ra.The improved hdlc-ec, independently from lipid levels, may be one of the missing links between inflammation, lipids, and cv risk in ra.

Journal: Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases (2022)
Publication types: Journal Article