syedzayyan/paper_mill_benchmark huggingface dataset:
Effect of Selected Biopesticides in Reducing Soybean Rust (Phakopsora pachyrhizi) Development.

The intensive use of fungicides in controlling soybean rust (sbr), a damaging foliar fungal disease of soybean caused by the obligate fungus phakopsora pachyrhizi, may have accelerated the insensitivity of p.Pachyrhizipopulations to fungicides.The objective of this study was to determine the effect of selected biopesticides and their application time on reducing sbr infection.There were differences (p< 0.05) in percent rust reduction values for application times, biopesticide treatments, and their interaction in detached-leaf and whole-plant greenhouse experiments.All application times and nearly all biopesticide treatments reduced (α=0.05) fungal infection compared with the nonfungicide control.Among the treatments,bacillus subtilis qst 713 and acibenzolar-s-methyl often reduced fungal sporulation more than the other treatments in detached-leaf and whole-plant greenhouse experiments.The identification of biopesticides effective to p.Pachyrhizimay be a valuable alternative or complement to synthetic fungicides and may be useful in integrated pest management programs for sbr control.

Journal: Plant disease (2019)
Publication types: Journal Article
Knowledge, Attitudes, and Behaviors Regarding Lyme Borreliosis Prevention in the Endemic Area of Northeastern Poland.

(1) background: the incidence of lyme borreliosis (lb) is increasing in europe.The new lb vaccine is still in clinical development, thus the dissemination of knowledge about the disease is essential.We assessed the knowledge, attitudes and preventive practices (kap) against tick-borne diseases (tbds) of people living in the endemic area in northeastern poland.(2) methods: we surveyed 406 adults using a 37-item anonymous paper survey.The data were analyzed with regression models.(3) results: the two most popular knowledge sources were the internet and doctors, selected by 77.8% and 53.4%, respectively.Respondents felt moderately knowledgeable about tbds and tick bite prophylaxis (median scores 5/10, and 6/10, respectively), considered tbds to be a significant health threat (median 8/10), attributed high risk to tick mouthparts remaining in the skin after tick removal (median 10/10), and shared multiple misconceptions regarding lb transmission, symptoms, and management.General knowledge scores (gks) about tbds and tick protection practices scores (tps) were moderate (65.0%; iqr, 55.8−71.7%, 63.6%; 54.5−72.7%, respectively).Only 48.0% had a positive attitude towards tbe vaccination.A recent tick-bite was associated with higher gks (or, 2.55; 95% ci, 1.27−5.10; p = 0.008), higher tps (or 4.76, 95% ci, 2.0−11.1; p < 0.001), and a positive attitude towards tbe vaccine (or 2.10, 1.07−4.10, p = 0.030).A positive vaccine attitude was also associated with obtaining tbd knowledge from doctors and other verified sources (or, 2.654, 1.66−4.23; p < 0.001).Age, place of residence, and frequent exposure to ticks in green areas were not associated with gks, tps, nor vaccine attitude.(4) conclusions: increased risk perceptions are associated with adoption of behaviors preventing tbds.Medical professionals play an important role in communicating knowledge about tbds.There is a need to revise current communication strategies with respect to tick bites and prevention of lb and other tbds.

Journal: Vaccines (2022)
Publication types: Journal Article
Tissue-resident memory T cells: The key frontier in local synovitis memory of rheumatoid arthritis.

Rheumatoid arthritis (ra) is a highly disabling, systemic autoimmune disease.It presents a remarkable tendency to recur, which renders it almost impossible for patients to live without drugs.Under such circumstances, many patients have to suffer the pain of recurrent attacks as well as the side effects of long-term medication.Current therapies for ra are primarily systemic treatments without targeting the problem that ra is more likely to recur locally.Emerging studies suggest the existence of a mechanism mediating local memory during ra, which is closely related to the persistent residence of tissue-resident memory t cells (trm).Trm, one of the memory t cell subsets, reside in tissues providing immediate immune protection but driving recurrent local inflammation on the other hand.The heterogeneity among synovial trmis unclear, with the dominated cd8+trmobserved in inflamed synovium of ra patients coming into focus.Besides local arthritis relapse, trmmay also contribute to extra-articular organ involvement in ra due to their migration potential.Future integration of single-cell rna sequencing (sc rna-seq) with spatial transcriptomics to explore the gene expression patterns of trmin both temporal dimension and spatial dimension may help us identify specific therapeutic targets.Targeting synovial trmto suppress local arthritis flares while using systemic therapies to prevent extra-articular organ involvement may provide a new perspective to address ra recurrence.

Journal: Journal of autoimmunity (2022)
Publication types: Journal Article;Review
Porous bimetallic cobalt-iron phosphide nanofoam for efficient and stable oxygen evolution catalysis.

Developing oxygen evolution reaction (oer) catalysts with high activity and long-term stability is critical to achieving efficient hydrogen production from water electrolysis.Herein, a porous bimetallic cobalt-iron phosphide (co fe-p) nanofoam is synthesized via a novel one-pot glucose-blowing followed by oxidization and then phosphidization process.The co fe-p nanofoam presents a porous nanostructure which contributes to contact with electrolytes and release of generated gas during electrocatalytic reactions.As oer catalysts in alkaline, the bimetallic porous co fe-p nanofoam exhibit a lower overpotential (258 m v@10 m a cm-2) as well as outstanding stability (70 h@100 m a cm-2), which surpasses the ru o2and is comparable to many high-performance co and fe-based catalysts.It is demonstrated that the surface of co fe-p undergo a reconstruction process and form new high active (cox fe1-x)ooh.Density functional theory (dft) calculations reveal that the elevated activity is caused by the bimetal co and fe optimizing the d-band center (ed) energy levels and thus balancing the adsorption-desorption capacities for oer intermediates.This work through constructing porous bimetallic nanofoam offers a feasible strategy to facilitate the reaction activity and prolong the long-term stability of oer.

Journal: Journal of colloid and interface science (2022)
Publication types: Journal Article
Disinfection methods of dental unit waterlines contamination: a systematic review.

Severe contamination of dental unit waterlines was found in healthcare settings.The benefits of decontamination methods are controversial.The aim of this review was to systematically evaluate disinfection methods in contamination control of dental unit waterlines.methods.the terms 'dental unit waterline(s) or duwl(s) or dental unit water line(s)' were searched through pub med, cochrane library, embase, web of science and scopusup to 31 may 2021.The duwls' output water was incubated on r2a agar at 20-28 °c for 5-7 days to evaluate heterotrophic mesophilic bacteria.The risk of bias was evaluated by a modified newcastle-ottawa quality assessment scale.results.eighteen papers from the literature were included.One study indicated that water supply played a crucial role in disinfecting duwls.Three studies indicated that flushing decreased bacteria counts but did not meet the american cdc standard (500 c.f.u.Ml-1).All chlorine- and peroxide-containing disinfectants except sodium hypochlorite in one of 15 studies as well as three mouthrinses and citrus botanical extract achieved the standard (≤500 c.f.u.Ml-1).The included studies were of low (1/18), moderate (6/18) and high (11/18) quality.conclusion.independent water reservoirs are recommended for disinfecting duwls using distilled water.Flushing duwls should be combined with disinfections.Nearly all the chlorine-, chlorhexidine- and peroxide-containing disinfectants, mouthrinses and citrus botanical extract meet the standard for disinfecting duwls.Alkaline peroxide would lead to tube blockage in the duwls.Regularly changing disinfectants can reduce the risk of occurrence of disinfectant-resistant strains of microbes.

Journal: Journal of medical microbiology (2022)
Publication types: Journal Article;Systematic Review
Periconception and Prenatal Exposure to Maternal Perceived Stress and Cord Blood DNA Methylation.

Maternal prenatal stress is associated with physiologic and adverse mental health outcomes in the offspring, but the underlying biologic mechanisms are unknown.We examined the associations of maternal perceived stress, including preconception exposure, with dna methylation (dnam) alterations in the cord blood buffy coats of 358 singleton infants.Methods maternal perceived stress was measured prior to and throughout pregnancy in a cohort of women enrolled in effects of aspirin in gestation and reproduction trial (eage r) trial.Perceived stress assessments based on a standardized likert-scale were obtained in periconception (~2 months preconception and 2-8 weeks of gestation) and pregnancy (8-36 weeks of gestation).Cumulative perceived stress was estimated by calculating the predicted area under the curve of stress reported prior to and during pregnancy.Dnam was measured by the infinium methylation epic bead chip.Multivariable robust linear regression was used to assess associations of perceived stress with individual cp g probes.Results based on a 0 to 3 scale, average reported preconception and early pregnancy stress were 0.76 (0.60) and 0.67 (0.50), respectively.Average mid- to late-pregnancy stress, based on a 0 to 10 scale, was 4.9 (1.6).Neither periconception nor pregnancy perceived stress were associated with individual cp g sites in neonatal cord blood (all false discovery rate [fdr] >5%).Conclusion no effects of maternal perceived stress exposure on array-wide cord blood neonatal methylation differences were found.

Journal: Epigenetics insights (2022)
Publication types: Journal Article
Th1 cytokines TNF-α and IFN-γ promote corticosteroid resistance in developing human airway smooth muscle.

Corticosteroids (css) are commonly used to manage wheezing and asthma in pediatric populations.Although corticosteroids are effective in alleviating airway diseases, some children with more moderate-severe asthma phenotypes show cs resistance and exhibit significant airflow obstruction, persistent inflammation, and more frequent exacerbations.Previous studies have demonstrated that th1 cytokines, such as tnf-α and ifn-γ, promote cs resistance in adult human airway smooth muscle (asm).In the present study, using a human fetal asm cell model, we tested the hypothesis that tnf-α/ifn-γ induces cs resistance.In contrast to tnf-α or ifn-γ alone, the combination of tnf-α/ifn-γ blunted the ability of fluticasone propionate (fp) to reduce expression of the chemokines ccl5 and cxcl10 despite expression of key anti-inflammatory glucocorticoid receptor target genes being largely unaffected by tnf-α/ifn-γ.Expression of the nf-κb subunit p65 and phosphorylation of stat1 were elevated in cells treated with tnf-α/ifn-γ, an effect that remained in the presence of fp.Si rna knockdown studies demonstrated the effects of tnf-α/ifn-γ on increased p65 are mediated by stat1, a transcription factor activated by ifn-γ.Expression of tnfaip3, a negative regulator of nf-κb activity, was not altered by tnf-α/ifn-γ.However, the effects of tnf-α/ifn-γ were partially reduced by overexpression of tnfaip3 but did not influence p65 expression.Together, these data suggest that ifn-γ augments the effects of tnf-α on chemokines by enhancing expression of key inflammatory pathways in the presence of cs.Interactions between tnf-α- and ifn-γ-mediated pathways may promote inflammation in asthmatic children resistant to css.

Journal: American journal of physiology. Lung cellular and molecular physiology (2019)
Publication types: Journal Article;Research Support, N.I.H., Extramural
Characterization of the physicochemical, thermal and rheological properties of cashew kernel starch.

The study aimed to characterize physicochemical, thermal, and rheological properties of cashew nut starch (cns) and then compare the obtained results with the properties of potato and corn starches.Cns showed higher gelatinization temperatures (112.29 °c) than those noted for potato and maize starches (78.44-94.65 °c).In addition, cns had higher peak viscosity (19.03 m pa·s) than high amylose corn starch.The static shear rheological test indicated that the cns followed a pseudoplastic behavior.In addition, cns sample showed a thixotropic patter, which was less pronounced than that observed for potato starch, but higher than the value reported for high amylose corn starch.These results demonstrated that the shear resistance of cns was lower than high amylose corn starch, but higher than potato starch.The storage and loss modulus (g' and g", respectively) of the cns were higher than those reported for the rest of samples.In this line, elastic properties were predominant in cns sample.In conclusion, results from this study provided insight into physicochemical and structural properties of cashew nut starch, which could represent a preliminary step for its future application in food processing.

Journal: Food chemistry: X (2022)
Publication types: Journal Article
Porphyromonas gingivalisOuter Membrane Vesicles Stimulate Gingival Epithelial Cells to Induce Pro-Inflammatory Cytokines via the MAPK and STING Pathways.

Porphyromonas gingivalis(pg) is a keystone pathogen associated with chronic periodontitis and produces outer membrane vesicles (omvs) that contain lipopolysaccharide (lps), gingipains, and pathogen-derived dna and rna.pg-omvs are involved in the pathogenesis of periodontitis.pg-omv-activated pathways that induce the production of the pro-inflammatory cytokines, interleukin (il)-6, and il-8 in the human gingival epithelial cell line, oba-9, were investigated.The role of mitogen-activated protein kinase (mapk) and nuclear factor (nf)-κb in levels of pg-omv-induced pro-inflammatory cytokines was investigated using western blot analysis and specific pathway inhibitors.pg-omvs induced il-6 and il-8 production via the extracellular signal-regulated kinase (erk) 1/2, c-jun n-terminal kinase (jnk), p38 mapk, and nf-κb signaling pathways in oba-9 cells.In addition, the stimulator of interferon genes (sting), an essential innate immune signaling molecule, was triggered by a cytosolic pathogen dna.pg-omv-induced il-6 and il-8 m rna expression and production were significantly suppressed by sting-specific small interfering rna.Taken together, these results demonstrated that pg-omv-activated erk1/2, jnk, p38 mapk, sting, and nf-κb signaling pathways resulting in increased il-6 and il-8 expression in human gingival epithelial cells.These results suggest that pg-omvs may play important roles in periodontitis exacerbation by stimulating various pathways.

Journal: Biomedicines (2022)
Publication types: Journal Article
Epitope Mapping of Anti-Mouse CCR3 Monoclonal Antibodies Using Flow Cytometry.

The cc chemokine receptor 3 (ccr3) is a receptor for cc chemokines, including ccl5/rantes, ccl7/mcp-3, and ccl11/eotaxin.Ccr3 is expressed on the surface of eosinophils, basophils, a subset of th2 lymphocytes, mast cells, and airway epithelial cells.Ccr3 and its ligands are involved in airway hyperresponsiveness in allergic asthma, ocular allergies, and cancers.Therefore, ccr3 is an attractive target for those therapies.Previously, anti-mouse ccr3 (m ccr3) monoclonal antibodies (m abs), c3mab-3 (rat ig g2a, kappa), and c3mab-4 (rat ig g2a, kappa) were developed using the cell-based immunization and screening (cbis) method.In this study, the binding epitope of these m abs was investigated using flow cytometry.A ccr3 extracellular domain-substituted mutant analysis showed that c3mab-3, c3mab-4, and a commercially available m ab (j073e5) recognized the n-terminal region (amino acids 1-38) of m ccr3.Next, alanine scanning was conducted in the n-terminal region.The results revealed that the ala2, phe3, asn4, and thr5 of m ccr3 are involved in c3mab-3 binding, whereas ala2, phe3, and thr5 are essential to c3mab-4 binding, and ala2 and phe3 are crucial to j073e5 binding.These results reveal the involvement of the n-terminus of m ccr3 in the recognition of c3mab-3, c3mab-4, and j073e5.

Journal: Antibodies (Basel, Switzerland) (2022)
Publication types: Journal Article
Harbor-border inspection for unmanned aerial vehicle based on visible light source tracking.

The unmanned aerial vehicle (uav) offers unique advantages of autonomous flight capability and small coefficient of risk, and is increasingly being used in harbor-border inspection to ensure security and orderly operation of harbors.In response to the influence of external factors such as electromagnetic interference in harbor-border inspection, this paper utilizes uav and visible light communication (vlc) to build an efficient system to track maritime targets near the harbor reliably.In a vlc scenario, a geometrical equation for transmitter positioning is first proposed based on the received signal strength of the optical signal emitted by the target.On this basis, linear iterative positioning (lip) using first-order taylor expansion is proposed to realize online beam tracking.Furthermore, quadratic approximative iterative positioning (qaip), a more precise approximation of the geometrical equation, is proposed based on second-order taylor expansion.Simulation results show that the proposed algorithms can track targets effectively, and qaip can achieve higher accuracy with no noise or high signal-to-noise ratio.In addition, compared with the geometrical solution, lip and qaip have faster computing speeds and fixed overheads.

Journal: Applied optics (2021)
Publication types: Journal Article
Applying ergonomics within the multi-modelling paradigm with an example from multiple UAV control.

This article presents a position statement on using ergonomics in conjunction with the multi-modelling paradigm.Multi-modelling is a computational approach to combine models of systems and components for design and simulation of cyber physical systems and systems of systems.Despite potentially significant benefits in terms of more human-centric system modelling, there is limited evidence of the application of ergonomics within multi-modelling.This article presents the case for applying ergonomics within multi-modelling.We open with an introduction to multi-modelling and benefits, applications and gaps for ergonomics in multi-modelling, and of potentially useful models from ergonomics.We then describe a proof-of-concept implementation of ergonomics within a multi-model of uav control.This demonstrates that as well as user-centred modelling, this approach supports ergonomics in how we can access rich systems models, and the collaborative value of applying ergonomics theory in systems design.practitioner summary:examines multi-modelling, a computational approach for complex modelling, and the contribution of ergonomics.An autonomous uav test implementation demonstrates the application of ergonomics knowledge for improving design and evaluation processes, and how multi-modelling can give ergonomics access to rich systems models.abbreviations:act-r: adaptive control of thought-rational; api: application programming interface; cfd: computational fluid dynamics; cots: commerical off the shelf; cps: cyber-physical system; ct: continuous time; de: discrete event; dse: design space exploration; fme: finite element modeling; fmi: functional mock-up interface; fmu: functional mock-up unit; goms: goals, operators, methods, selections; hci: human-computer interaction; imprint: improved performance research integration tool; into-cps: integrated toolchain for cyber-physical system modeling; klm: keystroke level model; mpc: model-predictive control; sys ml: system markup language; so s: system of system; uav: unmanned aerial vehicle.

Journal: Ergonomics (2020)
Publication types: Journal Article
Clinical and Autoimmune Characteristics of Severe and Critical Cases of COVID-19.

In this study we report on the clinical and autoimmune characteristics of severe and critical novel coronavirus pneumonia caused by severe acute respiratory syndrome-associated coronavirus 2 (sars-co v-2).The clinical, autoimmune, and laboratory characteristics of 21 patients who had laboratory-confirmed severe and critical cases of coronavirus disease 2019 (covid-19) from the intensive care unit of the huangshi central hospital, hubei province, china, were investigated.A total of 21 patients (13 men and 8 women), including 8 (38.1%) severe cases and 13 (61.9%) critical cases, were enrolled.Cough (90.5%) and fever (81.0%) were the dominant symptoms, and most patients (76.2%) had at least one coexisting disorder on admission.The most common characteristics on chest computed tomography were ground-glass opacity (100%) and bilateral patchy shadowing (76.2%).The most common findings on laboratory measurement were lymphocytopenia (85.7%) and elevated levels of c-reactive protein (94.7%) and interleukin-6 (89.5%).The prevalence of anti-52 k da ssa/ro antibody, anti-60 k da ssa/ro antibody, and antinuclear antibody was 20%, 25%, and 50%, respectively.We also retrospectively analyzed the clinical and laboratory data from 21 severe and critical cases of covid-19.Autoimmune phenomena exist in covid-19 subjects, and the present results provide the rationale for a strategy of preventing immune dysfunction and optimal immunosuppressive therapy.

Journal: Clinical and translational science (2020)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Molecular characterization of a putative alphapartitivirus from Impatiens balsamina L.

Two double stranded rnas (ds rnas) that likely represent the genome of an alphapartitivirus, tentatively named "impatiens cryptic virus 1" (icv1), were recovered from impatiens balsamina l.Rna1 (2008 bp) codes for the rna-dependent rna polymerase (rd rp) of icv1, which shares <83% amino acid sequence identity with the rd rps of other alphapartitiviruses.Rna2 (1906 bp) codes for the coat protein (cp) of icv1, which shares <60% amino acid sequence identity with the cps of other alphapartitiviruses.Phylogenetic analysis suggested that icv1 is closely related to plant alphapartitiviruses, including vicia cryptic virus, beet cryptic virus 1, carrot cryptic virus, and white clover cryptic virus 1.Using primers specific for rna1 or rna2, icv1 could be detected in i.Balsamina from various parts of china.

Journal: Archives of virology (2022)
Publication types: Journal Article
Functional cure of a chronic virus infection by shifting the virus - host equilibrium state.

The clinical handling of chronic virus infections remains a challenge.Here we describe recent progress in the understanding of virus - host interaction dynamics.Based on the systems biology concept of multi-stability and the prediction of multiplicative cooperativity between virus-specific cytotoxic t cells and neutralising antibodies, we argue for the requirements to engage multiple immune system components for functional cure strategies.Our arguments are derived from lcmv model system studies and are translated to hiv-1 infection.

Journal: Frontiers in immunology (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't