syedzayyan/paper_mill_benchmark huggingface dataset:
Towards an Effective and Safe Treatment of Inflammatory Pain: A Delphi-Guided Expert Consensus.

The clinical management of inflammatory pain requires an optimal balance between effective analgesia and associated safety risks.To date, mechanisms associated with inflammatory pain are not completely understood because of their complex nature and the involvement of both peripheral and central mechanisms.This expert consensus document is intended to update clinicians about evolving areas of clinical practice and/or available treatment options for the management of patients with inflammatory pain.Method an international group of experts in pain management covering the pharmacology, neurology and rheumatology fields carried out an independent qualitative systematic literature search using medline, embase and the cochrane central register of controlled trials.Results existing guidelines for pain management provide recommendations that do not satisfactorily address the complex nature of pain.To achieve optimal outcomes, drug choices should be individualized to guarantee the best match between the characteristics of the patient and the properties of the medication.Nsaids represent an important prescribing choice in the management of inflammatory pain, and the recent results on paracetamol question its appropriate use in clinical practice, raising the need for re-evaluation of the recommendations in the clinical practice guidelines.Conclusions increasing clinicians' knowledge of the available pharmacologic options to treat different pain mechanisms offers the potential for safe, individualized treatment decisions.We hope that it will help implement the needed changes in the management of inflammatory pain by providing the best strategies and new insights to achieve the ultimate goal of managing the disease and obtaining optimal benefits for patients.Funding dompé farmaceutici spa and paolo procacci foundation.

Journal: Advances in therapy (2019)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Transcatheter aortic valve implantation after aortic valve neocuspidization using autologous pericardium: a case report.

Aortic valve neocuspidization (avneo), a novel surgical procedure used in the treatment of aortic valve diseases, including aortic stenosis (as), involves the replacement of three aortic valve cusps by glutaraldehyde-treated autologous pericardium.Although reoperation risk is low, no case report on the deterioration of the avneo has yet been published.Case summary an 80-year-old woman who underwent avneo for severe degenerative tricuspid as 6 years previously complained of shortness of breath.Echocardiographic assessment revealed the reconstructed aortic valve leaflet was elongated, thickened, and marginally calcified resulting in recurrent severe as.Transcatheter aortic valve implantation using balloon-expandable transcatheter heart valve was successfully performed.Discussion to our knowledge, this is the first case report regarding the structural deterioration of the avneo resulting in restenosis 6 years after the first surgery.Transcatheter aortic valve implantation is possibly a suitable approach for post-procedural recurrence after avneo to avoid redo open-heart surgery which would be of prohibitive risk especially in an elderly population.

Journal: European heart journal. Case reports (2019)
Publication types: Case Reports;Journal Article
The Impact of Pre-Referral Advanced Diagnostic Testing on Wait Time to See a Hand Surgeon for Common Upper-Extremity Conditions.

We sought to evaluate the use of pre- and post-referral advanced diagnostic testing among patients with 3 common hand conditions, rates of subsequent tests, and differences in wait time to see a hand surgeon.Methods we analyzed a single academic tertiary care center administrative database of encounters from 2006 to 2015 to identify adult patients who were referred to a hand surgeon for 3 conditions (carpal tunnel syndrome [cts], soft tissue masses [stm], and joint pain [jp]).We recorded patient characteristics, use and timing of diagnostic tests, and wait time for the initial hand surgeon evaluation.Results among patients who received advanced diagnostic tests before the surgeon evaluation, cts patients had the highest rate of receiving pre-referral advanced testing (53.4%) compared with jp (10.6% ) and stm patients (5.8%).The cts patients had the highest rates of repeat testing (19.5%) compared with patients with jp (1.4%) and stm (0%).Across all 3 conditions, patients who received pre-referral advanced testing waited an additional 19 to 94 days to see a surgeon, compared with patients who received only post-referral testing or no testing.Conclusions use of pre-referral advanced diagnostic tests is associated with an increased time to see a hand surgeon for common hand conditions.Clinical relevance hand surgeons should have a role in identifying patients who do or do not benefit from advanced testing before referral to ensure that tests ordered before consultation are useful to both patients and treating surgeons.

Journal: The Journal of hand surgery (2019)
Publication types: Journal Article
Artificial decellularized extracellular matrix improves the regenerative capacity of adipose tissue derived stem cells on 3D printed polycaprolactone scaffolds.

Ideal tissue engineering frameworks should be both an optimal biological microenvironment and a shape and stability providing framework.In this study we tried to combine the advantages of cell-derived artificial extracellular matrix (ecm) with those of 3d printed polycaprolactone (pcl) scaffolds.In part a, both chondrogenic and osteogenic ecms were produced by human adipose derived stem cells (h ascs) on 3d-printed pcl scaffolds and then decellularized to create cell free functionalized pcl scaffolds, named ac pcl and ao pcl respectively.The decellularization resulted in a significant reduction of the dna content as well as the removal of nuclei while the ecm was largely preserved.In part b the bioactivation and the effect of the ac/ao pcl scaffolds on the proliferation, differentiation, and gene expression of h ascs was investigated.The ac/ao pcl scaffolds were found to be non-toxic and allow good adhesion, but do not affect proliferation.In the in vitro investigation of cartilage regeneration, biochemical analysis showed that ac pcl scaffolds have an additional effect on chondrogenic differentiation as gene expression analysis showed markers of cartilage hypertrophy.The ao pcl showed a large influence on the differentiation of h ascs.In control medium they were able to stimulate h ascs to produce calcium alone and all genes relevant investigated for osteogenesis were significantly higher expressed on ao pcl than on unmodified pcl.Therefore, we believe that ac/ao pcl scaffolds have a high potential to improve regenerative capacity of unmodified pcl scaffolds and should be further investigated.

Journal: Journal of tissue engineering (2021)
Publication types: Journal Article
Elevated ubiquitination contributes to protective immunity against severe SARS-CoV-2 infection.

The crosstalk between the ubiquitin-proteasome and the immune system plays an important role in the health and pathogenesis of viral infection.However, there have been few studies of ubiquitin activation in severe acute respiratory syndrome coronavirus 2 (sars-co v-2) infection.Methods we investigated the effect of ubiquitination on sars-co v-2 infection and patient prognosis by integrating published coronavirus disease 2019 (covid-19) multi-transcriptome data and bioinformatics methods.Results the differential expression of covid-19 samples revealed changed ubiquitination in most solid and hollow organs, and it was activated in lymphatic and other immune tissues.In addition, in the respiratory system of covid-19 patients, the immune response was mainly focused on the alveoli, and the expression of ubiquitination reflected increasing immune infiltration.Ubiquitination stratification could significantly differentiate patients' prognosis and inflammation levels through the general transcriptional analysis of the peripheral blood of patients with covid-19.Moreover, high ubiquitination levels were associated with a favourable prognosis, low inflammatory response, and reduced mechanical ventilation and intensive care unit.Moreover, high ubiquitination promoted a beneficial immune response while inhibiting immune damage.Finally, prognostic stratification and biomarker screening based on ubiquitination traits played an important role in clinical management and drug development.Conclusion ubiquitination characteristics provides new ideas for clinical intervention and prognostic guidance for covid-19 patients.

Journal: Clinical and translational medicine (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Effects of Daily Consumption of an Aqueous Dispersion of Free-Phytosterols Nanoparticles on Individuals with Metabolic Syndrome: A Randomised, Double-Blind, Placebo-Controlled Clinical Trial.

Metabolic syndrome (ms) affects up to 40% of the population and is associated with heart failure, stroke and diabetes.Phytosterols (ps) could help to manage one or more ms criteria.The purpose of this study was to evaluate the therapeutic effect of daily supplementation of an aqueous dispersion of 2 g of free-phytosterols nanoparticles in individuals with ms over six months of intervention, compared with placebo.This double-blind study included 202 participants with ms randomly assigned into phytosterol (n= 102) and placebo (n= 100) groups.Participants were assessed at baseline, 4, 12 and 24 weeks.General health questions, anthropometric measurements and blood parameters were analysed.At week 24, the proportion of participants with high triglycerides (≥150 mg/d l) in the phytosterol group was 15.65% lower than in the placebo group (p-value = 0.023).Similarly, half of the participants in the phytosterol group decreased their waist circumference up to 4 cm compared with 0 cm in the placebo group (p-value = 0.0001).We reported no adverse effects (diarrhoea or vitamin d reduction); nonetheless, almost 70% of participants in the phytosterol group self-reported an improvement in bowel habits.Daily intake of free-ps nanoparticles improved some ms criteria; therefore, it might be a promising adjuvant therapy for individuals with ms (nct02969720).

Journal: Nutrients (2020)
Publication types: Journal Article;Randomized Controlled Trial
Metagenomic Quantification of Genes with Internal Standards.

We demonstrate that an assembly-independent and spike-in facilitated metagenomic quantification approach can be used to screen and quantify over 2,000 genes simultaneously, while delivering absolute gene concentrations comparable to those for quantitative pcr (q pcr).Dna extracted from dairy manure slurry, digestate, and compost was spiked with genomic dna from a marine bacterium and sequenced using the illumina hi seq4000.We compared gene copy concentrations, in gene copies per mass of sample, of five antimicrobial resistance genes (args) generated with (i) our quantitative metagenomic approach, (ii) targeted q pcr, and (iii) a hybrid quantification approach involving metagenomics and q pcr-based 16s r rna gene quantification.Although q pcr achieved lower quantification limits, the metagenomic method avoided biases caused by primer specificity inherent to q pcr-based methods and was able to detect orders of magnitude more genes than is possible with q pcr assays.We used the approach to simultaneously quantify args in the comprehensive antimicrobial resistance database (card).We observed that the total abundance of tetracycline resistance genes was consistent across different stages of manure treatment on three farms, but different samples were dominated by different tetracycline resistance gene families.importanceq pcr and metagenomics are central molecular techniques that have offered insights into biological processes for decades, from monitoring spatial and temporal gene dynamics to tracking args or pathogens.Still needed is a tool that can quantify thousands of relevant genes in a sample as gene copies per sample mass or volume.We compare a quantitative metagenomic approach with traditional q pcr approaches in the quantification of arg targets in dairy manure samples.By leveraging the benefits of nontargeted community genomics, we demonstrate high-throughput absolute gene quantification of all known arg sequences in environmental samples.

Journal: mBio (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't;Research Support, U.S. Gov't, Non-P.H.S.
Green Revolution to Gene Revolution: Technological Advances in Agriculture to Feed the World.

Technological applications in agriculture have evolved substantially to increase crop yields and quality to meet global food demand.Conventional techniques, such as seed saving, selective breeding, and mutation breeding (variation breeding), have dramatically increased crop production, especially during the 'green revolution' in the 1990s.However, newer issues, such as limited arable lands, climate change, and ever-increasing food demand, pose challenges to agricultural production and threaten food security.In the following 'gene revolution' era, rapid innovations in the biotechnology field provide alternative strategies to further improve crop yield, quality, and resilience towards biotic and abiotic stresses.These innovations include the introduction of dna recombinant technology and applications of genome editing techniques, such as transcription activator-like effector (talen), zinc-finger nucleases (zfn), and clustered regularly interspaced short palindromic repeats/crispr associated (crispr/cas) systems.However, the acceptance and future of these modern tools rely on the regulatory frameworks governing their development and production in various countries.Herein, we examine the evolution of technological applications in agriculture, focusing on the motivations for their introduction, technical challenges, possible benefits and concerns, and regulatory frameworks governing genetically engineered product development and production.

Journal: Plants (Basel, Switzerland) (2022)
Publication types: Journal Article;Review
Intersectional Structural Stigma, Community Priorities, and Opportunities for Transgender Health Equity: Findings from TRANSforming the Carolinas.

In this manuscript, "intersectional structural stigma, community priorities, and opportunities for transgender health equity," poteat and simmons outline the legal and policy barriers that impede efforts to end the hiv epidemic among transgender people in the south.They present qualitative and quantitative data from a community engaged research study conducted with transgender adults and other key stakeholders as well as finding from an analysis of policies impacting transgender people in both states.Violence prevention and decriminalization are highlighted as key policy initiatives that would advance health equity for transgender people.

Journal: The Journal of law, medicine & ethics : a journal of the American Society of Law, Medicine & Ethics (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural
Cerebellar Contributions to Motor and Cognitive Control in Multiple Sclerosis✰✰✰.

To evaluate relationships between specific cerebellar regions and common clinical measures of motor and cognitive function in persons with multiple sclerosis (pw ms).Design cross-sectional.Setting laboratory.Participants twenty-nine pw ms and 28 age- and sex-matched controls without multiple sclerosis (ms) (n=57).Interventions not applicable.Main outcome measures both diffusion and lobule magnetic resonance imaging analyses and common clinical measures of motor and cognitive function were used to examine structure-function relationships in the cerebellum.Results pw ms demonstrate significantly worse motor and cognitive function than controls, including weaker strength, slower walking, and poorer performance on the symbol digit modalities test, but demonstrate no differences in cerebellar volume.However, pw ms demonstrate significantly worse diffusivity (mean diffusivity: p=.0003; axial diffusivity: p=.0015; radial diffusivity: p=.0005; fractional anisotropy: p=.016) of the superior cerebellar peduncle, the primary output of the cerebellum.Increased volume of the motor lobules (i-v, viii) was significantly related to better motor (p<.022) and cognitive (p=.046) performance, and increased volume of the cognitive lobules (vi-vii) was also related to better motor (p<.032) and cognitive (p=.008) performance, supporting the role of the cerebellum in both motor and cognitive functioning.Conclusions these data highlight the contributions of the cerebellum to both motor and cognitive function in pw ms.Using novel neuroimaging techniques to examine structure-function relationships in pw ms improves our understanding of individualized differences in this heterogeneous group and may provide an avenue for targeted, individualized rehabilitation aimed at improving cerebellar dysfunction in ms.

Journal: Archives of physical medicine and rehabilitation (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Ban-xia-xie-xin-tang ameliorates hepatic steatosis by regulating Cidea and Cidec expression in HFD-fed mice.

Ban-xia-xie-xin-tang (bxxxt) has been applied in treating metabolic diseases, such as nonalcohol fatty liver disease, diabetes mellitus, and obesity.However, the underlying molecular mechanism of bxxxt in treating diabetes mellitus is unknown.Purpose to clarify the underlying molecular mechanism of bxxxt in alleviating hepatic steatosis in high-fat diet (hfd)-fed mice.Methods after 12 weeks of hfd treatment, mice were administered bxxxt for 4 weeks.The main chemical components of bxxxt were identified by uplc-tq-ms/ms.Indicators associated with insulin resistance and lipid metabolism were detected.The effect of improving glucose and lipid metabolism between bxxxt and the different components was compared.Differentially expressed genes (degs) were identified by hepatic transcriptomics.Key degs and proteins were further detected by real-time quantitative polymerase chain reaction, western blotting, immunohistochemistry, and immunofluorescence staining.Lds and mitochondria were detected by transmission electron microscopy.Results first of all, our data demonstrated that the capacity to improve glucose and lipid metabolism for bxxxt was significantly superior to different components of bxxxt.Bxxxt was found to improve hfd-induced insulin resistance.Moreover, bxxxt decreased weight, serum/hepatic triglycerides, total cholesterol, and ffas to alleviate hfd-induced hepatic steatosis.According to the results of the hepatic transcription, cidea and cidec were identified as critical degs for promoting ld fusion and reducing ffas β-oxidation in mitochondria and peroxisome resulting in hepatic steatosis, which was reversed by bxxxt.Conclusion bxxxt ameliorates hfd-induced hepatic steatosis and insulin resistance by increasing cidea and cidec-mediated mitochondrial and peroxisomal fatty acid oxidation, which may provide a potential strategy for therapy of nafld and t2dm.

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (2022)
Publication types: Journal Article
Complexin I knockout rats exhibit a complex neurobehavioral phenotype including profound ataxia and marked deficits in lifespan.

Complexin i (cplx1), a presynaptic small molecule protein, forms snare complex in the central nervous system involved in the anchoring, pre-excitation, and fusion of axonal end vesicles.Abnormal expression of cplx1 occurs in several neurodegenerative and psychiatric disorders that exhibit disrupted neurobehaviors.Cplx1 gene knockout induces severe ataxia and social behavioral deficits in mice, which has been poorly demonstrated.Here, to address the limitations of single-species models and to provide translational insights relevant to human diseases, we used cplx1 knockout rats to further explore the function of the cplx1 gene.The crispr/cas9 gene editing system was adopted to generate cplx1 knockout rats (cplx1-/-).Then, we characterized the survival rate and behavioral phenotype of cplx1-/-rats using behavioral analysis.To further explain this phenomenon, we performed blood glucose testing, nissl staining, hematoxylin-eosin staining, and golgi staining.We found that cplx1-/-rats showed profound ataxia, dystonia, movement and exploratory deficits, and increased anxiety and sensory deficits but had normal cognitive function.Nevertheless, cplx1-/-rats could swim without training.The abnormal histomorphology of the stomach and intestine were related to decreased weight and early death in these rats.Decreased dendritic branching was also found in spinal motor neurons in cplx1-/-rats.In conclusion, cplx1 gene knockout induced the abnormal histomorphology of the stomach and intestine and decreased dendritic branching in spinal motor neurons, causing different phenotypes between cplx1-/-rats and mice, even though both of these phenotypes showed profound ataxia.These findings provide a new perspective for understanding the role of cplx1.

Journal: Pflugers Archiv : European journal of physiology (2020)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Neural and self-report indices of cognitive reappraisal moderate the association between sensitivity to uncertain threat and problem alcohol use.

Exaggerated reactivity to threats that are uncertain (u-threat) is a risk factor for problem alcohol use.Data suggest that exaggerated reactivity to u-threat is associated with chronic anxiety and motivation for coping-oriented drinking.Not all individuals with high u-threat reactivity engage in excessive drinking and theory and research suggest that individual differences in emotion regulation, particularly frequency and effectiveness of cognitive reappraisal, are potential moderators of this well-established link.The aim of the current study was to test this hypothesis using a multimodal laboratory design.A total of 83 volunteers with depression and/or anxiety completed a well-validated threat sensitivity task and two complimentary assessments of cognitive reappraisal.Threat sensitivity was measured using startle eyeblink potentiation during threat-of-electric shock.Cognitive reappraisal was measured using self-report and estimates of prefrontal cortex activation (pfc; i.e., ventrolateral, dorsolateral and dorsomedial) during instructed reappraisal during functional magnetic resonance imaging.Results revealed self-reported and pfc indices of reappraisal were correlated within-subjects.Additionally, self-reported and ventrolateral (vl pfc) activation during reappraisal moderated the association between reactivity to u-threat and problem alcohol use.Across both measures, at low engagement in reappraisal, greater startle reactivity to u-threat was associated with greater problem alcohol use.At high engagement in reappraisal, there was no association between u-threat reactivity and problem alcohol use.Together, the findings reveal that exaggerated reactivity to u-threat may be a particularly robust risk factor for problem alcohol use in the context of impaired or ineffective emotion regulation.

Journal: International journal of psychophysiology : official journal of the International Organization of Psychophysiology (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural