syedzayyan/paper_mill_benchmark huggingface dataset:
eConsult Specialist Quality of Response (eSQUARE): A novel tool to measure specialist correspondence via electronic consultation.

High-quality correspondence between healthcare providers is critical for effective patient care.We developed an assessment tool to measure the quality of specialist correspondence to primary care providers (pcps) via electronic consultation (e consult), where specialists provide advice without specialist-patient interactions.We incorporated fourteen previously described features of high-quality e consult correspondence into an assessment tool named the e consult specialist quality of response (e square).Six pcps and two specialists applied the 10-item e square tool to 30 e consults of varying quality as informed by pcp survey data.Content, response process, and internal structure validity evidence was gathered.Psychometric properties were calculated using descriptive statistics and generalizability analyses.Mean total score for low-quality e consults (m = 24 ± 5.6) was significantly lower than moderate-quality e consults (m = 38 ± 4.7; p<0.001) which was significantly lower than high-quality e consults (m = 46 ± 3.0; p = 0.002).Reliability measures were high, including generalizability coefficient (0.96), inter-item (≥0.55) and item-total correlations (≥0.68).A decision study demonstrated that a single rater was adequate to achieve a reliability measure of ≥0.70.This study demonstrates initial validity evidence including multiple reliability measures for the e square.A single rater is adequate to achieve reliability measures for formative feedback.Future studies can apply the e square when planning educational initiatives aiming to improve specialist-to-pcp correspondence via e consult.

Journal: Journal of telemedicine and telecare (2022)
Publication types: Journal Article
Functional consequences of extended high frequency hearing impairment: Evidence from the speech, spatial, and qualities of hearing scale.

Hearing loss in the extended high frequencies, despite a normal audiogram, could affect speech-in-noise recognition.However, it is not known if extended high frequency (ehf) hearing loss is associated with everyday listening and communication deficits.The present study aimed to determine the functional effects of impaired ehf hearing among adults using the speech, spatial, and qualities of hearing scale (ssq).A secondary objective was to evaluate the relationship between objective (speech-in-noise recognition) and subjective (ssq) measures of hearing function.Listeners with ehf hearing loss provided lower ssq ratings compared with their ehf-normal counterparts.The lower ratings could not be attributed to the age or standard audiogram of the listeners.Ratings from more than 50% of ehf-impaired listeners were below the 2 sd cutoff point obtained from ehf-normal listeners.The mean speech recognition threshold was poorer for ehf-impaired listeners, and a poorer speech recognition threshold was associated with lower ssq ratings, i.e., poorer self-reported hearing ability.For some listeners, ehf hearing loss could be the missing link between self-reported hearing difficulty in the presence of a normal audiogram.These findings provide evidence for the functional deficits associated with ehf hearing loss and invoke the need for future investigations into the management of ehf loss.

Journal: The Journal of the Acoustical Society of America (2022)
Publication types: Journal Article
Enhancement of transamniotic stem cell therapy for spina bifida by genetic engineering of donor mesenchymal stem cells with an Fgf2 transgene.

/purpose we examined whether engineered overexpression of fibroblast growth factor-2 (fgf2) in donor mesenchymal stem cells (mscs) could enhance spina bifida coverage induced by transamniotic stem cell therapy (trascet).Methods pregnant sprague-dawley dams (n = 24) exposed to retinoic acid for induction of fetal spina bifida were divided in three groups.An untreated group had no further manipulations.Two groups received volume-matched intra-amniotic injections into all fetuses (n = 157) of either amniotic fluid-derived mscs (af msc; n = 85) or af mscs transduced with an fgf2 transgene (fgf2-af msc; n = 72) on gestational day 17 (term=21-22 days).Defect coverage was categorized at term by histology and pan-cytokeratin immunohistochemistry.Statistical coverage comparisons were by logistic regression.Results among 84 survivors with isolated spina bifida, 71 had definitive histology.Defect coverage rates in both the af msc (38.5%) and fgf2-af msc (73.3%) groups were statistically significantly higher than in the untreated group (10%; p<0.001 for both).There was a significantly higher coverage rate in the fgf2-af msc group compared with the af msc group (p = 0.025).Conclusions fgf2 overexpression in donor mesenchymal stem cells increases defect coverage rates in a rodent model of transamniotic stem cell therapy for spina bifida.Genetic engineering of donor cells is a promising strategy for the enhancement of this emerging therapy.

Journal: Journal of pediatric surgery (2021)
Publication types: Journal Article
Corneal Collagen Crosslinking (CXL) for Corneal Ectasia after Small Incision Lenticule Extraction (SMILE).

With an estimated incidence of 0.011%, the smile procedure seems to have the lowest risk of postoperative keratectasia among contemporary keratorefractive procedures.Nevertheless, due to the novelty of the procedure as well as the lack of data, no clear superiority over femto-lasik or prk can be stated at this time.In this respect, application of the identical tomographic screening criteria previously developed for excimer-based procedures is of paramount importance to minimize the risk of corneal ectasia.As an adjunct to conventional corneal tomography, newer imaging modalities such as oct-based epithelial mapping should be used for preoperative screening before keratorefractive surgery.Corneal crosslinking is an established treatment modality for post-smile keratectasia, which promises high success rates especially in early stages.The present case report illustrates these diagnostic and therapeutic considerations.

Journal: Klinische Monatsblatter fur Augenheilkunde (2022)
Publication types: Case Reports;Journal Article
Noteworthy effect of slight variation in aliphatic chain length of trisubstituted imidazole inhibitors against epidermal growth factor receptor L858R/T790M/C797S mutant in cancer therapy.

11h is a very potent inhibitor against epidermal growth factor receptor triple mutation l858r/t790m/c797s (egfrtm) with 13-fold stronger potency than the fda-approved osimertinib.Recently, two new egfrtminhibitors, 11d and 11e, were reported which revealed 2.8- and 2.3-fold stronger potency than 11h, respectively.11h, 11d, and 11e have the same structures but differ only in their aliphatic chain length.However, the exact effects of differential aliphatic chain length on the inhibitory potencies of these compounds require further elaboration at the atomistic level, hence the objective of this report.Various computational tools were employed for this purpose.From our findings, it was revealed that van der waals (vd w) interactions modulate the binding mechanisms of these inhibitors and play the most important role in the differential inhibitory activities of 11d, 11h, and 11e.The strong hydrogen bond formation between the aliphatic chain of 11d and key residue arg841 was recognized as the reason for its higher activity and inhibitory potency relative to 11h and 11e.Moreover, the extension of the n-terminal loop into the active site for vd w interaction with the phenyl group of 11e and carbon-hydrogen bond formed between the aliphatic chain of 11e and leu718 engendered a higher activity of 11e than 11h.

Journal: Chemical biology & drug design (2019)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Produced β-hydroxybutyrate after β-hydroxy-β-methylbutyrate (HMB) administration may contribute HMB function in mice.

Β-hydroxy-β-methylbutyrate (hmb) is an intermediate in the metabolism of the branched-chain amino acid leucine.Hmb has several demonstrated effects on skeletal muscle function, some of which are contradictory.In addition, the effect of exogenous hmb intake on the levels of intermediate metabolites is not known.Therefore, we investigated changes in hmb metabolites after oral hmb administration in mice.First, icr mice were treated with either distilled water or hmb (0.215 g/10 m l/kg).Sampling was performed at 0, 1, 6, 12, and 24 h after administration.Next, icr mice were given distilled water or hmb (0.215 g/10 m l/kg/d) for 10 d.Mice given hmb shown a significant increase in liver β-methylcrotonyl-co a and increased β-hydroxybutyrate in plasma and the gastrocnemius muscle 1 h after hmb administration.Mice administered hmb for 10 d showed significantly decreased food intake and body weight; however, the relative weight of the gastrocnemius muscle was significantly increased.These results may be attributed to an increase in β-hydroxybutyrate resulting from exogenous hmb, since β-hydroxybutyrate inhibits food intake and suppresses skeletal muscle catabolism.In conclusion, β-hydroxybutyrate, a metabolite of hmb, was found to play an important role in the function of hmb.

Journal: Biochemistry and biophysics reports (2021)
Publication types: Journal Article
Three-component Castagnoli-Cushman reaction with ammonium acetate delivers 2-unsubstituted isoquinol-1-ones as potent inhibitors of poly(ADP-ribose) polymerase (PARP).

An earlier described three-component variant of the castagnoli-cushman reaction employing homophthalic anhydrides, carbonyl compound and ammonium acetate was applied towards the preparation of 1-oxo-3,4-dihydroisoquinoline-4-carboxamides with variable substituent in position 3.These compounds displayed inhibitory activity towards poly(adp-ribose) polymerase (parp), a clinically validated cancer target.The most potent compound (parp1/2 ic50= 22/4.0 n m) displayed the highest selectivity towards parp2 in the series (selectivity index = 5.5), more advantageous adme prameters compared to the clinically used parp inhibitor olaparib.

Journal: Journal of enzyme inhibition and medicinal chemistry (2021)
Publication types: Journal Article
Flow patterns in circular fish tanks and its relations with flow rate and nozzle features.

The main aim of this research is to study the effect of flow rate, diameter and number of nozzles on the rotational velocity, impulse force and average velocity of water in circular fish tanks.The results indicated that, the rotational velocity increases with increasing flow rates from 5 to 75 m3h-1by using 10 and 15 mm nozzles diameter with nozzles number of 5, 10, 15, 20, 25 and 30.The same results were also obtained when 20 and 25 mm nozzle diameter with nozzles number of 3, 6, 9, 12 and 15 were used.The impulse force of water decreases with increasing nozzle diameter from 10.0 to 15.0 mm with 5, 10, 15, 20, 25 and 30 nozzles and from 20.0 to 25.0 mm with 3, 6, 9, 12 and 15 nozzles.When the nozzle diameter increased from 10 to 25 mm the impulse force of water decreased with 15 nozzles.The average velocity of water decreases with increasing nozzles diameter from 10.0 to 15.0 mm with 5, 10, 15, 20, 25 and 30 nozzles and from 20.0 to 25.0 mm with 3, 6, 9, 12 and 15 nozzles.When the nozzle diameter increased from 10 to 25 mm, the average velocity ranged from 1.07 to 48.76 cm s-1for all treatments under study.

Journal: Scientific reports (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
β-Cyclodextrin and Oligoarginine Peptide-Based Dendrimer-Entrapped Gold Nanoparticles for Improving Drug Delivery to the Inner Ear.

Here, we developed a safe and highly effective nanocarrier using β-cyclodextrin (β-cd) and oligoarginine peptide (arg8)-modified dendrimer-entrapped gold nanoparticles (au@cd-pamam-arg8), with a diameter of 5 nm, for improved delivery of dexamethasone (dex) to the inner ear.The properties andin vivodistribution of the au@cd-pamam-arg8 were assessedin vitro, and a streptomycin (sm) ototoxicity model was usedin vivo.Flow cytometry analysis of heioc1 cells treated with au@cd-pamam-arg8 and au @cd-pamam at different time intervals indicated that cell uptake efficiency of the drug delivery carrier au@cd-pamam-arg8 was higher than that of au @cd-pamam.Au@cd-pamam-arg8 carrying dex (au@cd-pamam-arg8/dex) were mainly distributed in hair cells, the spiral ganglion, lateral wall, and nerve fibers and had stronger protective effects on the inner ear than dex administration alone.in vivotracer tests revealed that tympanic injection was significantly more effective than posterior ear injection, muscle injection, and tail vein injection, whereas clinical retro-auricular injection could not increase the efficiency of drug delivery into the ear.Electrocochleography results showed that au@cd-pamam-arg8/dex significantly improved hearing in c57/bl6 mice after sm exposure.These findings indicate that au@cd-pamam-arg8 may be the useful drug carriers for the treatment of inner ear diseases.

Journal: Frontiers in bioengineering and biotechnology (2022)
Publication types: Journal Article
Food Security, Access, and Challenges in New Mexico during COVID-19.

The novel coronavirus disease 2019 (covid-19) pandemic disrupted food systems and economies across the united states.Public health measures, including stay-at-home orders, led to employment disruptions and food system shocks that increased barriers to food access.Objectives we aimed to examine food insecurity and food access challenges in new mexico (nm) during the covid-19 pandemic.Methods a cross-sectional study using a validated survey was conducted in nm in may and june 2020.Adults 18 y and older were recruited through convenience sampling via email, websites, and targeted social media ads from major universities, nongovernmental organizations, state agencies, and media outlets.Survey questions assessed food insecurity and food-related challenges and worry.Bivariate and multivariate logistic regression examined relations between food insecurity and demographic characteristics.z tests were used to compare the proportions of individuals who responded affirmatively to food challenge and worry questions between food-secure and food-insecure respondents.Results a total of 1487 residents participated in the study.Thirty percent of respondents reported experiencing food insecurity and 16% experienced very low food security since the pandemic started.Food insecurity was associated with each of 7 characteristics examined in bivariate logistic regression analyses.Multivariate logistic regression results showed that hispanic (adjusted or: 1.70; 95% ci: 1.18, 2.44) and female (adjusted or: 1.78; 95% ci: 1.09, 2.90) respondents were more likely to experience food insecurity than non-hispanic white and male respondents.Larger household sizes were associated with higher odds of food insecurity except for those in the lowest and highest income categories.z tests showed that a higher proportion of food-insecure respondents experienced food-related challenges and worry than food-secure respondents.Conclusions disparities in food insecurity persisted during the covid-19 pandemic and food-insecure individuals were more likely to report experiencing food-related challenges and worry.Researchers and policy makers in nm may consider continuing efforts to mitigate food access issues as the pandemic continues.

Journal: Current developments in nutrition (2022)
Publication types: Journal Article
Fabrication of Gradient Nanopattern by Thermal Nanoimprinting Technique and Screening of the Response of Human Endothelial Colony-forming Cells.

Nanotopography can be found in various extracellular matrices (ecms) around the body and is known to have important regulatory actions upon cellular reactions.However, it is difficult to determine the relation between the size of a nanostructure and the responses of cells owing to the lack of proper screening tools.Here, we show the development of reproducible and cost-effective gradient nanopattern plates for the manipulation of cellular responses.Using anodic aluminum oxide (aao) as a master mold, gradient nanopattern plates with nanopillars of increasing diameter ranges [120-200 nm (gp 120/200), 200-280 nm (gp 200/280), and 280-360 nm (gp 280/360)] were fabricated by a thermal imprinting technique.These gradient nanopattern plates were designed to mimic the various sizes of nanotopography in the ecm and were used to screen the responses of human endothelial colony-forming cells (h ecfcs).In this protocol, we describe the step-by-step procedure of fabricating gradient nanopattern plates for cell engineering, techniques of cultivating h ecfcs from human peripheral blood, and culturing h ecfcs on nanopattern plates.

Journal: Journal of visualized experiments : JoVE (2018)
Publication types: Journal Article;Research Support, Non-U.S. Gov't;Video-Audio Media
Association Between the Gut Microbiome and Their Metabolites With Human Blood Pressure Variability.

Blood pressure (bp) variability is an independent risk factor for cardiovascular events.Recent evidence supports a role for the gut microbiota in bp regulation.However, whether the gut microbiome is associated with bp variability is yet to be determined.Here, we aimed to investigate the interplay between the gut microbiome and their metabolites in relation to bp variability.Methods ambulatory bp monitoring was performed in 69 participants from australia (55.1% women; mean±sd, 59.8±7.26 years; body mass index, 25.2±2.83 kg/m2).These data were used to determine nighttime dipping, morning bp surge (mbps) and bp variability as sd.The gut microbiome was determined by 16s ribosomal rna (r rna) sequencing and metabolite levels by gas chromatography.Results we identified specific taxa associated with systolic bp variability, nighttime dipping, and mbps.Notably,alistipesfinegoldiiand lactobacillusspp.Were only present in participants within the normal ranges of bp variability, mbps and dipping, while prevotellaspp.And clostridiumspp., were found to be present in extreme dippers and the highest quartiles of bp sd and mbps.There was a negative association between mbps and microbial α-diversity (r=-0.244,p=0.046).Mbps was also negatively associated with plasma levels of microbial metabolites called short-chain fatty acids (r=-0.305,p=0.020), particularly acetate (r=-0.311,p=0.017).Conclusions gut microbiome diversity, levels of microbial metabolites, and the bacteria alistipesfinegoldiiand lactobacilluswere associated with lower bp variability and clostridiumand prevotellawith higher bp variability.Thus, our findings suggest the gut microbiome and metabolites may be involved in the regulation of bp variability.

Journal: Hypertension (Dallas, Tex. : 1979) (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Improved sample treatment for the determination of flavonoids and polyphenols in sweet potato leaves by ultra performance convergence chromatography-tandem mass spectrometry.

This work reports the development and application of a multi-compound analysis method for the determination of 17 flavonoids and polyphenols in sweet potato leaves.Samples were processed by microwave-enhanced accelerated solvent extraction under low pressure at 120 °c for 10 min.Salting-out homogeneous liquid-liquid extraction was used to remove water and water-soluble impurities from the extract before ultra performance convergence chromatography-tandem mass spectrometry analysis.By using the supercritical fluid (co2and methanol) as the mobile phase, the 17 components and the internal standard were well separated in 10 min without obvious matrix effect.Method validation was performed and good accuracy (relative error in the range of ±3.6%) and precision (inter- and intra-day relative standard deviations<10.9%) were obtained.The limits of detection were in the range of 0.1-0.6 ng ∙ m l-1.Recoveries were assessed at three concentration levels and acceptable mean values (76.1-101.5%) were found.This method has also been used to analyze ten different samples, and the average concentrations of components were determined in the range of 4.9-218.6 mg/100 g dry weight.The results showed the method could be helpful for the analysis of flavonoids and polyphenols in plant leaves.

Journal: Journal of pharmaceutical and biomedical analysis (2019)
Publication types: Journal Article
Biosimilar competition: Early learning.

Biologics accounted for roughly $145 billion in spending in 2018.They are also the fastest growing segment of the pharmaceutical industry.The biological price competition and innovation act (bpcia) of 2010 created an abbreviated pathway for biosimilar products to promote price competition in the market for biological drugs.There was great anticipation that the bpcia would lead to a moderation in drug prices driven by market competition.The observed levels of competition and the accompanying savings have not reached those expected levels.We investigate the early impacts of biosimilar competition on the use and pricing of biological products.We focus especially on the ways in which altered market structures stemming from the implementation of the bpcia have affected the prices for biological products subject to biosimilar competition.We do so by studying seven products that have recently faced biosimilar competition.We estimate fixed effects and instrumental variables models to estimate the impact of market competition on prices.Our results indicate that in the range of one to three entrants each additional marketed product results in a reduction in weighted average market prices of between 5.4% and 7% points.These are the result of a combination of reductions in originator prices and shifting in demand to biosimilar products.

Journal: Health economics (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Degradation Behavior, Biocompatibility, Electrochemical Performance, and Circularity Potential of Transient Batteries.

Transient technology seeks the development of materials, devices, or systems that undergo controlled degradation processes after a stable operation period, leaving behind harmless residues.To enable externally powered fully transient devices operating for longer periods compared to passive devices, transient batteries are needed.Albeit transient batteries are initially intended for biomedical applications, they represent an effective solution to circumvent the current contaminant leakage into the environment.Transient technology enables a more efficient recycling as it enhances material retrieval rates, limiting both human and environmental exposures to the hazardous pollutants present in conventional batteries.Little efforts are focused to catalog and understand the degradation characteristics of transient batteries.As the energy field is a property-driven science, not only electrochemical performance but also their degradation behavior plays a pivotal role in defining the specific end-use applications.The state-of-the-art transient batteries are critically reviewed with special emphasis on the degradation mechanisms, transiency time, and biocompatibility of the released degradation products.The potential of transient batteries to change the current paradigm that considers batteries as harmful waste is highlighted.Overall, transient batteries are ready for takeoff and hold a promising future to be a frontrunner in the uptake of circular economy concepts.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2021)
Publication types: Journal Article;Review;Research Support, Non-U.S. Gov't