syedzayyan/paper_mill_benchmark huggingface dataset:
Interactions of isoorientin and its Semi-synthetic analogs with human serum albumin.

Isoorientin is a c-glycosyl flavone with a wide range of health beneficial effects and inhibits glycogen synthase kinase 3β (gsk-3β) potentially against alzheimer's disease.Its semi-synthetic derivatives have greater potency than isoorientin.The present study was aimed to determine the mechanism of interactions of isoorientin and its derivatives with human serum albumin (hsa) using multi-spectroscopic, microscale thermophoresis (mst) and computational studies.Spectra of steady-state fluorescence, uv-vis, and time-resolved fluorescence indicated that isoorientin and its derivatives quenched the intrinsic fluorescence of hsa through a static quenching process.Isoorientin and its derivatives had a moderate affinity with hsa (ka7.7-14.9 × 104m-1).The binding process was accompanied by an exothermic phenomenon, δg° of hsa-isoorientin and its derivatives systems were calculated as from -29.51 k j mol-1to -27.87 k j mol-1.Displacement experiments with site-specific markers revealed that isoorientin and its derivatives bind to hsa at site ii (subdomain iiia) only.A reduction in the α-helical content of hsa-isoorientin and its derivatives complex was observed, because the conformational changes was structurally perturbed by the hydrophilic groups of the compounds.Further molecular modeling studies confirmed that the binding of isoorientin and its derivatives to the site ii via hydrophobic interaction.The mst results confirmed the interactions between hsa and the compounds of interest.The esterase-like assay studies indicated that isoorientin and its derivatives shared the same binding site in hsa, and their induced structural changes of hsa may have been caused by partial unfolding of hsa.This work helps to understand transport, distribution, bioactivity, and design of flavonoid-based gsk-3β inhibitors.

Journal: Bioorganic chemistry (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
In vivo cartilage tissue engineering.

Biologic treatment options for cartilage injuries require chondrocyte expansion using cell culture.Clinical application is accomplished in two surgical sessions and is expensive.If isolation of chondrocytes and stimulus for proliferation and extracellular matrix synthesis can be achieved in vivo, the treatment can be performed in one session and the cost can be reduced.Methods a 2.5-cm diameter full-thickness chondral defect was created in the knees of five groups of sheep.In one group, some of the chondral tissues obtained from the creation of the defect were diced into small pieces and were placed into the defect and were covered with a collagen membrane (miv group).In the other group, the collagen membrane was soaked in collagenase prior to usage.In the next group, the collagen membrane was soaked in both collagenase and growth factors.Matrix-induced autologous chondrocyte implantation (maci) was applied to another group in two sessions, and the last group was left untreated.After 15 weeks of follow-up, repair tissues were compared macroscopically, histomorphometrically, and biochemically for tissue concentrations of glycosaminoglycan and type ii collagen.Results maci and miv groups demonstrated better healing than others and were similar.Addition of collagenase or growth factors did not improve the results.Addition of collagenase did not have detrimental effect on the surrounding cartilage.Conclusions with the described method, it is possible to obtain comparable results with maci.Further studies are also needed to see if it works similarly in humans.

Journal: Journal of orthopaedic surgery and research (2018)
Publication types: Journal Article
Maternal Worry About Infant Weight and its Influence on Artificial Milk Supplementation and Breastfeeding Cessation.

Maternal worry about infant weight has inconsistently been reported as a breastfeeding barrier.Weight monitoring is a critical tool to assess adequacy of infant feeding.Yet, little is known about the intensity of maternal worry about infant weight or associated breastfeeding outcomes.Research aims to examine (1) the frequency and intensity of maternal worry about infant weight; (2) the relationship between worry about weight and use of artificial milk; and 3) the relationship between worry about weight and breastfeeding cessation.Methods a prospective cross-sectional design was used.A questionnaire was completed by women in the united states (n= 287) from 12 web-based maternal support groups.Results sixty-three percent of women (n= 182) had some worry about infant weight.Participants breastfeeding for the first time had more worry (p= .035).Participants still breastfeeding had less worry about weight compared to those who had stopped (67%,n= 147 vs.41%,n= 28).Exclusive breastfeeding participants had less worry (p< .001) compared to those who supplemented with artificial milk.Increased worry was associated with the use of artificial milk within 1 week of birth (p< .001) and early breastfeeding cessation (p< .001).Conclusions worry about weight is a significant breastfeeding barrier.It is associated with first time breastfeeding, less exclusive breastfeeding, use of artificial milk, and earlier breastfeeding cessation.Lactating mothers need anticipatory guidance about expected neonatal weight changes and interventions to help relieve worry about infant weight.

Journal: Journal of human lactation : official journal of International Lactation Consultant Association (2022)
Publication types: Journal Article
Feasibility and Acceptability of Ecological Momentary Assessment With Young Adults Who Are Currently or Were Formerly Homeless: Mixed Methods Study.

Ecological momentary assessment (ema) has been used with young people experiencing homelessness to gather information on contexts associated with homelessness and risk behavior in real time and has proven feasible in this population.However, the extent to which ema may affect the attitudes or behaviors of young adults who are currently or were formerly homeless and are residing in supportive housing has not been well investigated.Objective this study aims to describe the feedback regarding ema study participation from young adults who are currently or were formerly homeless and examine the reactivity to ema participation and compliance.Methods this mixed methods study used cross-sectional data collected before and after ema, intensive longitudinal data from a 7-day ema prompting period, and focus groups of young adults who are currently or were formerly homeless in los angeles, california, between 2017 and 2019.Results qualitative data confirmed the quantitative findings.Differences in the experience of ema between young adults who are currently or were formerly homeless were found to be related to stress or anxiety, interference with daily life, difficulty charging, behavior change, and honesty in responses.Anxiety and depression symptomatology decreased from before to after ema; however, compliance was not significantly associated with this decrease.Conclusions the results point to special considerations when administering ema to young adults who are currently or were formerly homeless.Ema appears to be slightly more burdensome for young adults who are currently homeless than for those residing in supportive housing, which are nuances to consider in the study design.The lack of a relationship between study compliance and symptomatology suggests low levels of reactivity.

Journal: JMIR formative research (2022)
Publication types: Journal Article
Multiple microwave frequency measurement system based on a sawtooth-wave-modulated non-flat optical frequency comb.

A photonic-assisted instantaneous microwave measurement system, capable of measuring multiple frequency signals, is demonstrated and analyzed.The principle lies in the combination of a channelizer and frequency-to-power mapping.An effective generation method of a non-flat optical frequency comb is proposed based on sawtooth wave modulation, which has more comb lines and adjustable comb spacing.Under this method, two low-speed post-processing devices are utilized to realize frequency measurements up to 32 ghz.The scheme is verified by simulation, and factors affecting system performance are also studied.

Journal: Applied optics (2022)
Publication types: Journal Article
Errata.

Haefeli pc, marecek gs, keel mjb, siebenrock ka, tannast m.patients undergoing surgical hip dislocation for the treatment of acetabular fractures show favourable long-term outcome.bone joint j;2017;99-b:508-515.

Journal: The bone & joint journal (2017)
Publication types: Journal Article;Published Erratum
C─C Cleavage Approach to C─H Functionalization of Saturated Aza-Cycles.

Saturated cyclic amines (aza-cycles) are ubiquitous structural motifs found in pharmaceuticals, agrochemicals, and bioactive natural products.Given their importance, methods that directly functionalize aza-cycles are in high demand.Herein, we disclose a fundamentally different approach to functionalizing cyclic amines which relies on c─c cleavage and attendant cross-coupling.The initial functionalization step is the generation of underexplored n-fused bicycloα-hydroxy-β-lactams under mild, visible light conditions using a norrish-yang process to affectα-functionalization of saturated cyclic amines.This approach is complementary to previous methods for the c─h functionalization of aza-cycles and provides unique access to various cross-coupling adducts.In the course of these studies, we have also uncovered an orthogonal, base-promoted opening of the n-fused bicycloα-hydroxy-β-lactams.Computational studies have provided insight into the origin of the complementary c─c cleavage processes.

Journal: ACS catalysis (2020)
Publication types: Journal Article
Generation of Definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury.

In this chapter, we first describe two interchangeable protocols optimized in our lab for deriving definitive neuronal progenitor cells from human pluripotent stem cells (h pscs).The resultant npcs can then be propagated and differentiated to produce differing proportions of neurons, oligodendrocytes, and astrocytes as required for in vitro cell culture studies or in vivo transplantation.Following these protocols, we explain the method for transplanting these cells into the rat model of spinal cord injury (sci).

Journal: Methods in molecular biology (Clifton, N.J.) (2019)
Publication types: Journal Article
A Smart Archive Box for Museum Artifact Monitoring Using Battery-Less Temperature and Humidity Sensing.

For the first time, this paper reports a smart museum archive box that features a fully integrated wireless powered temperature and humidity sensor.The smart archive box has been specifically developed for microclimate environmental monitoring of stored museum artifacts in cultural heritage applications.The developed sensor does not require a battery and is wirelessly powered using near field communications (nfc).The proposed solution enables a convenient means for wireless sensing with the operator by simply placing a standard smartphone in close proximity to the cardboard archive box.Wireless sensing capability has the advantage of enabling long-term environmental monitoring of the contents of the archive box without having to move and open the box for reading or battery replacement.This contributes to a sustainable preventive conservation strategy and avoids the risk of exposing the contents to the external environment, which may result in degradation of the stored artifacts.In this work, a low-cost and fully integrated nfc sensor has been successfully developed and demonstrated.The developed sensor is capable of wirelessly measuring temperature and relative humidity with a mean error of 0.37 °c and ±0.35%, respectively.The design has also been optimized for low power operation with a measured peak dc power consumption of 900 μw while yielding a 4.5 cm wireless communication range.The power consumption of the nfc sensor is one of the lowest found in the literature.To the author's knowledge, the nfc sensor proposed in this paper is the first reporting of a smart archive box that is wirelessly powered and uniquely integrated within a cardboard archive box.

Journal: Sensors (Basel, Switzerland) (2021)
Publication types: Journal Article
Hair Cortisol, Testosterone, Dehydroepiandrosterone Sulfate and Their Ratios in Stallions as a Retrospective Measure of Hypothalamic-Pituitary-Adrenal and Hypothalamic-Pituitary-Gonadal Axes Activity: Exploring the Influence of Seasonality.

The monitoring of stress physiology includes studying a wide range of endocrinological mechanisms, which can be assessed using multiple tissue samples.This study aimed to evaluate the seasonal variations of hair c, t and dhea-s in horses for a whole year, as well as to assess the variations between seasons of c/dhea-s and t/c ratios as a retrospective measure of the hypothalamic-pituitary-adrenal and hypothalamic-pituitary-gonadal axis activity.Ten pure-breed menorca stallions were included in the study.The hair samples were collected approximately every two months following the shave-reshave method caudally to the sternum.After a methanol-based extraction, samples were analyzed by enzyme immunoassay for cortisol, testosterone, and dehydroepiandrosterone sulphate.Following our findings, we detected that cortisol, testosterone and dehydroepiandrosterone sulphate were significantly affected by seasonality, with the highest values of cortisol during summer and the lowest values of testosterone during spring.Dehydroepiandrosterone sulphate concentrations were increased in autumn compared to the other studied periods.Additionally, the studied hormone ratios showed variations between seasons.To conclude, season should, therefore, be considered when assessing sexual and stress hormones in stallion hair, since this variable can be a potential influencing factor and led to misinterpretations.

Journal: Animals : an open access journal from MDPI (2021)
Publication types: Journal Article
AGata3enhancer necessary for ILC2 development and function.

The type 2 helper effector program is driven by the master transcription factor gata3 and can be expressed by subsets of both innate lymphoid cells (ilcs) and adaptive cd4+t helper (th) cells.While ilc2s and th2 cells acquire their type 2 differentiation program under very different contexts, the distinct regulatory mechanisms governing this common program are only partially understood.Here we show that the differentiation of ilc2s, and their concomitant high level of gata3 expression, are controlled by a gata3enhancer,gata3+674/762, that plays only a minimal role in th2 cell differentiation.Mice lacking this enhancer exhibited defects in several but not all type 2 inflammatory responses, depending on the respective degree of ilc2 and th2 cell involvement.Our study provides molecular insights into the different gene regulatory pathways leading to the acquisition of the gata3-driven type 2 helper effector program in innate and adaptive lymphocytes.

Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Publication types: Journal Article;Research Support, N.I.H., Extramural;Research Support, Non-U.S. Gov't
Axillary Vein Spasm During Permanent Pacemaker Implantation.

This case example illustrates the challenging nature of managing axillary vein spasm.Once axillary vein spasm has been diagnosed, unnecessary attempts at venous puncture should be avoided as this can result in worsening of the spasm.Intravenous fluids should be infused from the ipsilateral venous access.Intravenous nitroglycerin from the ipsilateral access in incremental doses of 100-200 μg can help in relieving the spasm.It is imperative to wait for 5-10 minutes before re-attempting puncture.A repeat venogram should be performed to demonstrate resolution of spasm and guide the puncture.If spasm persists, puncture may be attempted in the medial part of the axillary vein or the subclavian vein.In refractory cases where the above measures fail, contralateral venous access may be required.

Journal: The Journal of invasive cardiology (2022)
Publication types: Journal Article
Weight-bearing exercise prevents skeletal muscle atrophy in ovariectomized rats.

Skeletal muscle atrophy (sma) is a dominant symptom induced by estrogen deficiency which can lead to severe health problems of postmenopausal women.Furthermore, estrogen deficiency has severely compromised the maintenance of muscle stem cells as well as impairs self-renewal and differentiation into muscle fibers.Resistance training is commonly considered as a positive and useful intervention in accelerating the rate of muscle growth.As one of the resistance training, whether the weight-bearing exercise can alleviate sma induced by estrogen deficiency has not been investigated.The rats were divided into 3 groups randomly: sham group, ovariectomized (ovx) group, and weight-bearing exercise (wbe) therapeutic group.The weight that rats were loaded was 35% of their body weight, and the rats were trained by treadmill training (5° slope, 20 m/min, 30 min/day, 6 days/week) for 8 weeks.After training, the quality and strength of skeletal muscle of the wbe rats were improved; meanwhile, the cross-sectional areas of the skeletal muscle were also increased.Moreover, the wbe activated akt significantly, upregulated the expression of m tor, and downregulated the expression of mstn and its receptor act riib and fox o1, respectively.The sma phenomena of rats which induced by estrogen deficiency were prevented effectively via wbe, and the mstn/akt/m tor and fox o1 signaling pathway may be the predominant way in this improvement.

Journal: Journal of physiology and biochemistry (2021)
Publication types: Journal Article
A highly stable l-alanine-based mono(aquated) Mn(ii) complex as a T1-weighted MRI contrast agent.

The synthesized lithium (s)-6,6'-(1-carboxyethylazanediyl)bis(methylene)dipicolinate (li3cbda) is a new chiral, alanine-based ligand bearing two picolinate functionalities.The trianionic form of the ligand [(cbda)3-] constitutes a seven-coordinate, water-soluble, pentagonal bipyramidal mn(ii) complex (1).The structural analysis reveals the presence of a water coordinating site in the complex.The complex is thermodynamically very stable, and the stability is not affected by the presence of physiological anions (hco3-, po43-, and f-).The p h of the medium exerts a small effect on the stability of the complex.The r1relaxivity of 3.02 m m-1s-1is exhibited by the complex at 1.41 t, p h ∼7.4, and 25 °c.Phantom images obtained via a clinical mri brivo mr355 system established concentration-dependent signal enhancement by the complex.The cytotoxicity test confirmed complex 1 as a biocompatible potential t1-weighted mri contrast agent.

Journal: Dalton transactions (Cambridge, England : 2003) (2017)
Publication types: Journal Article
High-temperature magnetic skyrmions in BiCrX3(X = Se and Te) monolayers.

Two-dimensional magnetic van der waals materials provide a fertile platform for the design and control of topological spin textures such as skyrmions.However, despite studies reporting skyrmions in many 2d magnetic systems, those of the hosting van der waals materials remain limited.Here,viafirst-principles calculations and monte carlo simulations, we propose bi cr x3as a new family of materials for hosting skyrmions.Due to the large soc of the x atom and intrinsic inversion asymmetry, an inherent large dmi occurs in all systems, enabling intriguing néel-type skyrmions.Furthermore, upon applying a moderate magnetic field, isolated skyrmions and skyrmion lattices emerge in our systems, and are robust within a relatively wide temperature range.

Journal: Physical chemistry chemical physics : PCCP (2022)
Publication types: Journal Article
The Incidence of Venous Thromboembolism and Practice of Deep Venous Thrombosis Prophylaxis Among Hospitalized Cirrhotic Patients.

Patients with liver cirrhosis have altered hepatic synthetic functions which theoretically result in reduced levels of pro-and anti-coagulant factors as well as thrombocytopenia.Initially, cirrhotic patients were thought to be at an increased risk of bleeding and a reduced risk of thrombosis.Several studies have recently reported an increased occurrence of venous thromboembolism (vte) in cirrhotic patients.In this study, we aimed to assess the current practice of deep venous thrombosis (dvt) prophylaxis, the incidence and predictors of vte, and the associated bleeding sequelae in patients with liver cirrhosis.Methods a retrospective cohort study was performed.We included all adult patients with a diagnosis of liver cirrhosis from january 2010 to june 2019 admitted to the hospital.Our cohort patients were divided into two groups, cirrhotic patients with pharmacological vte prophylaxis and those with mechanical or no vte prophylaxis.Results we included 601 cirrhotic patients in our study.The incidence of vte occurring within the first 6 months of their admission was 1.5%.Seven patients (1.49%) developed vte with the majority being dvts while not on pharmacologic prophylaxis, and two patients developed vte despite being on pharmacologic prophylaxis; however, there was no statistical difference.Alcohol use was the most common underlying cause of liver cirrhosis (40.4%), followed by chronic hepatitis c (21.1%), and nonalcoholic steatohepatitis (11.3%).Out of the 601 patients included, 69 patients received neither pharmacologic nor mechanical vte prophylactic agent (11.48%), while the remaining majority received either pharmacological or mechanical prophylaxis (88.52%).Conclusions our study did not show a statistically significant association between the use of pharmacological vte prophylactic agents and a reduction in the risk of vte in cirrhotic patients.The rates of usage of dvt prophylactic agents among our northwell hospitals during the study period appeared to be no longer suboptimal when compared to prior studies.Low albumin appears to be a predictor factor to develop vte.There was a statistically significant increase in bleeding risk and transfusion requirement in cirrhotic patients receiving no pharmacological vte prophylactic agents.Further prospective trials are needed to shed more light on this subject and identify the group of cirrhotic patients who could safely benefit from pharmacologic vte prophylaxis.

Journal: Gastroenterology research (2022)
Publication types: Journal Article