syedzayyan/paper_mill_benchmark huggingface dataset:
Cycling-related trauma admissions to the major trauma centre in the cycling capital of the United Kingdom.

This study aims to characterise cycling related injuries presenting to a major trauma centre located within a region with the highest rates of cycling in the uk.Methods a retrospective analysis of cycling related trauma admissions occurring between january 2012 and june 2020 was performed.Our institution's electronic patient record system was used to collect relevant data for analysis including age, gender, mechanism of injury, glasgow coma scale (gcs) on arrival, incident date and time, injured body regions, 30-day mortality, helmet use and intubation rate.Comparison was made between groups of patients based on mechanism of injury.Results a total of 605 cycling related trauma cases were identified, with 52 being excluded due to incomplete data.The most common mechanism was 'fall from cycle' (53.5%).The 'cyclist v vehicle' group was associated with a significantly higher injury severity score (iss), lower gcs and higher intubation rate.Helmet wearers were significantly older than non-wearers and helmet use was associated with a significantly reduced risk of head injury, lower iss and intubation rate and a higher gcs.Discussion with a likely increase in future cycling uptake, it is crucial that effective interventions are implemented to improve the safety of cyclists.The findings of this study may be used to guide any such intervention.A multi-faceted strategy involving driver and cyclist education, effective road infrastructure changes and helmet promotion campaigns specifically targeting the younger generation could be employed.

Journal: Injury (2022)
Publication types: Journal Article
Ruthenium-Catalyzed α-Alkylation of Ketones Using Secondary Alcohols to β-Disubstituted Ketones.

An assortment of aromatic ketones was successfully functionalized with a variety of unactivated secondary alcohols that serve as alkylating agents, providing β-disubstituted ketone products in good to excellent yields.Remarkably, challenging substrates such as simple acetophenone derivatives are effectively alkylated under this ruthenium catalysis.The substituted cyclohexanol compounds displayed product-induced diastereoselectivity.Mechanistic studies indicate the involvement of the hydrogen-borrowing pathway in these alkylation reactions.Notably, this selective and catalytic c-c bond-forming reaction requires only a minimal load of catalyst and base and produces h2o as the only byproduct, making this protocol attractive and environmentally benign.

Journal: Organic letters (2020)
Publication types: Journal Article
Botryoid rhabdomyosarcoma of the uterine cervix: Report case.

Rhabdomyosarcoma of the cervix is among the rare histological types of cervical cancers, which are usually seen in young girls or women in the general active period.The usual circumstances of diagnosis are dominated by recurrent metrorrhagia.At the initial stage, the lesions may be asymptomatic or take on the appearance of a benign polyp.In the case reported here, the clinical symptomatology was recurrent metrorrhagia with a cervical implanted polyp occurring 28 months after resection of a benign polyp.Rms is one of the malignant tumors of mesenchymal origin.Typically, it is a malignant tumor proliferation of cells with morphological and/or phenotypic striated muscle differentiation.The characteristic cells of this tumor are rhabdomyoblasts rhabdomyosarcomas are classified into three histological subtypes: embryonal, alveolar, and anaplastic.Within embryonal rms, it is possible to distinguish between botryoid, leiomyomatous and anaplastic forms.Botryoid and leiomyomatous forms are classically described as having a more favorable prognosis.Treatment is based on a multidisciplinary approach that includes indications for conservative surgery, chemotherapy, radiotherapy and brachytherapy.

Journal: International journal of surgery case reports (2022)
Publication types: Journal Article
Maximal respiratory pressure in healthy Japanese children.

[purpose] normal values for respiratory muscle pressures during development in japanese children have not been reported.The purpose of this study was to investigate respiratory muscle pressures in japanese children aged 3-12 years.[subjects and methods] we measured respiratory muscle pressure values using a manovacuometer without a nose clip, with subjects in a sitting position.Data were collected for ages 3-6 (group i: 68 subjects), 7-9 (group ii: 86 subjects), and 10-12 (group iii: 64 subjects) years.[results] the values for respiratory muscle pressures in children were significantly higher with age in both sexes, and were higher in boys than in girls.Correlation coefficients were significant at values of 0.279 to 0.471 for each gender relationship between maximal respiratory pressure and age, height, and weight, respectively.[conclusion] in this study, we showed pediatric respiratory muscle pressure reference value for each age.In the present study, values for respiratory muscle pressures were lower than brazilian studies.This suggests that differences in respiratory muscle pressures vary with ethnicity.

Journal: Journal of physical therapy science (2017)
Publication types: Journal Article
TieguanyinOolong Tea Extracts Alleviate Behavioral Abnormalities by Modulating Neuroinflammation in APP/PS1 Mouse Model of Alzheimer's Disease.

Alzheimer's disease (ad) is a common neurodegenerative disease; tea components have important neuroprotective effects.This article explores the effects and mechanisms of qingxiang tiguanyin(tgy-q), nongxiang tieguanyin(tgy-n), and chenxiang tieguanyin(tgy-c) extracts on app/ps1 ad model mice.Morris water maze and new object recognition experiments show that tieguanyinextracts can effectively enhance the cognitive ability of app/ps1 mice.H&e staining, nissl staining, and immunohistochemical staining show that tieguanyinextracts make nerve cell boundaries and nucleoli become clearer, relieve nucleus pyknosis, and effectively reduce aβ1-40 and aβ1-42 in the hippocampus and cortex.They also restore the morphology of microglia and astrocytes.In addition,tieguanyinextracts can balance the oxidative stress level in the brain of app/ps1 mice by improving the antioxidant capacity.Western blot results show that tieguanyinextracts can reduce the expression of nf-κb p65, tnf-α, il-1β, il-6, cox-2, and i nos in mouse brain, which demonstrates that tieguanyinextracts improves cognitive ability by alleviating inflammation.This article demonstrates for the first time that tieguanyinextracts can inhibit the excessive activation of the nf-κb p65 signaling pathway and improve the antioxidant capacity in the cerebral cortex and hippocampus, to improve the cognitive ability of app/ps1 mice.Our results shed light into the beneficial of tieguanyintea extracts on preventing and alleviating ad diseases.

Journal: Foods (Basel, Switzerland) (2021)
Publication types: Journal Article
Chitosan/polycaprolactone multilayer hydrogel: A sustained Kartogenin delivery model for cartilage regeneration.

Cartilage regeneration using biomaterial-guided delivery systems presents improved therapeutic efficacy of the biomolecules while minimizing side effects.Here, our hypothesis was to design a multilayer scaffold of chitosan (cs) hydrogel and polycaprolactone (pcl) mat to enhance the mechanical properties, integrity and stability of cs, especially for subsequent in vivo transplantation.After conjugation of the kartogenin (kgn) into this structure, its gradual release can promote chondrogenesis of mesenchymal stem cells (mscs).Initially, a thin electrospun pcl layer was sandwiched between two cs hydrogels.Subsequently, kgn was superficially immobilized onto the cs matrix.The successful conjugation was confirmed by scanning electron microscopy (sem) and infrared spectroscopy.These novel kgn-conjugated scaffolds possessed lower swelling and higher compressive modulus and showed gradual release of kgn in longer retention times.Immunofluorescent and histological staining represented more cells located in lacunae as well as more coll2 and sox9 positive cells on kgn-conjugated scaffolds.Gene expression analysis also revealed that sox9, coll2 and acan expression levels were higher in the presence of kgn, while collx expression was down-regulated, indicating a hypertrophy phenomenon with synergistic effect of tgf-β.This multilayer structure not only facilitates the effective treatment, but also provides a proper mechanical structure for cartilage engineering.

Journal: International journal of biological macromolecules (2021)
Publication types: Journal Article
Physical multimorbidity and cancer prevalence in the National Health and Nutrition Examination Survey.

S as the us population ages, both cancer and multimorbidity become more common and pose challenges to the healthcare system.Limited studies have examined the association between multimorbidity and cancer prevalence in the us adult population.To help address this gap, we evaluated the associations between individual chronic conditions and all-site cancer, multimorbidity and all-site cancer, and multimorbidity and site-specific cancers.Study design this is a cross-sectional study.Methods data from 10,731 adults aged 20 years or older who participated in the 2013-2016 national health and nutrition examination survey were used in our study.Self-reported demographics, smoking status, sedentary behavior, body mass index, individual chronic conditions, multimorbidity status, cancer history, and cancer sites were assessed.Results in our sample, the prevalence of having any type of cancer or multimorbidity was 9% (n = 861) and 38% (n = 4248), respectively.Respiratory conditions (multivariable-adjusted odds ratio [or]: 1.3; 95% confidence interval [ci]: 1.1-1.6) and arthritis (multivariable-adjusted or: 1.5; 95% ci: 1.2-1.8) were observed to be statistically significantly associated with having all-site cancer after adjusting for potential confounders.Having multimorbidity was also statistically significantly associated with having all-site cancer (multivariable-adjusted or: 1.4; 95% ci: 1.2-1.7), cervical cancer (multivariable-adjusted or: 2.6; 95% ci: 1.2-5.4), and bladder cancer (multivariable-adjusted or: 2.8; 95% ci: 1.0-7.6).Conclusions multimorbidity was associated with all-site cancer, cervical cancer, and bladder cancer.The present study provides new evidence of the potential relationships between multimorbidity and cancer.Future longitudinal studies are warranted to clarify the temporality and potential biological mechanisms of the associations between multimorbidity and cancer.

Journal: Public health (2021)
Publication types: Journal Article
BCL2 G quadruplex-binding small molecules: Current status and prospects for the development of next-generation anticancer therapeutics.

B cell lymphoma 2 (bcl2) overexpression in a range of human tumors is often related to chemotherapy resistance and poor prognosis.Gc-rich regions upstream of the p1 promoter in human bcl2 can form g-quadruplex (g4) structures through the stacking of four hoogsteen-paired guanine bases.Stabilizing the g4 fold implies the inhibition of bcl2 expression and, thus, small molecules that selectively bind to the g4 are promising anticancer candidates.In this review, we discuss the structural aspects, binding affinity, selectivity, and biological activity of well-characterized bcl2 g4 binding ligands in vitro and in vivo.We also explore future directions in the research and development of g4-based anticancer therapeutics.

Journal: Drug discovery today (2022)
Publication types: Journal Article;Review;Research Support, Non-U.S. Gov't
Photoaffinity Labeling-Based Chemoproteomic Strategy Reveals RBBP4 as a Cellular Target of Protopanaxadiol against Colorectal Cancer Cells.

Protopanaxadiol (ppd), a main ginseng metabolite, exerts powerful anticancer effects against multiple types of cancer; however, its cellular targets remain elusive.Here, we synthesized a cell-permeable ppd probe via introducing a bifunctional alkyne-containing diazirine photo-crosslinker and performed a photoaffinity labeling-based chemoproteomic study.We identified retinoblastoma binding protein 4 (rbbp4), a chromatin remodeling factor, as an essential cellular target of ppd in hct116 colorectal cancer cells.Ppd significantly decreased rbbp4-dependent trimethylation at lysine 27 of histone h3 (h3k27me3), a crucial epigenetic marker that correlates with histologic signs of colorectal cancer aggressiveness, and ppd inhibition of proliferation and migration of hct116 cells was antagonized by rbbp4 rna silencing.Collectively, our study highlights a previously undisclosed anti-colorectal cancer cellular target of the ginseng metabolite and advances the fundamental understanding of rbbp4 functions via a chemical biology strategy.

Journal: Chembiochem : a European journal of chemical biology (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
IBD Systems Biology Is Here to Stay.

Systems biology is a rapidly advancing field of science that allows us to look into disease mechanisms, patient diagnosis and stratification, and drug development in a completely new light.It is based on the utilization of unbiased computational systems free of the traditional experimental approaches based on personal choices of what is important and what select experiments should be performed to obtain the expected results.Methods systems biology can be applied to inflammatory bowel disease (ibd) by learning basic concepts of omes and omics and how omics-derived "big data" can be integrated to discover the biological networks underlying highly complex diseases like ibd.Once these biological networks (interactomes) are identified, then the molecules controlling the disease network can be singled out and specific blockers developed.Results the field of systems biology in ibd is just emerging, and there is still limited information on how to best utilize its power to advance our understanding of crohn disease and ulcerative colitis to develop novel therapeutic strategies.Few centers have embraced systems biology in ibd, but the creation of international consortia and large biobanks will make biosamples available to basic and clinical ibd investigators for further research studies.Conclusions the implementation of systems biology is indispensable and unavoidable, and the patient and medical communities will both benefit immensely from what it will offer in the near future.

Journal: Inflammatory bowel diseases (2021)
Publication types: Journal Article
Antimicrobial Resistance of Clinical and CommensalEscherichia coliCanine Isolates: Profile Characterization and Comparison of Antimicrobial Susceptibility Results According to Different Guidelines.

Pyometra is a diestrual chronic disease frequently associated with escherichia coli.Initial pyometra treatment involves empiric antimicrobial therapy whose suitability should be confirmed by antimicrobial susceptibility testing.Antimicrobial resistance is a major health issue for veterinary medicine, rendering surveillance studies essential.Our goal was to determine the susceptibility profile of e.Coliisolates obtained from healthy and pyometra-presenting dogs and to compare the application of different antimicrobial susceptibility guidelines.Methods the antimicrobial susceptibility profile (asp) of 74e.Coliisolates was determined by disk diffusion, using six antimicrobials commonly used in veterinary medicine.Profiles were assessed by clsi vet01s, clsi m100 and eucast guidelines.Β-lactamases-encoding genesbla tem,bla shvandbla oxawere detected by multiplex pcr.Biofilm production ability was evaluated by pellicle formation assays in luria-bertani medium.Results variations in the resistance frequency were observed for amoxicillin/clavulanic acid, cephalexin and cefotaxime (29.7-54.1%, 10.8-16.2% and 1.4-4.1%, respectively).Results varied slightly between clinical and commensal isolates, as well as their biofilm-forming ability.Genesbla tem,bla shvandbla oxawere detected in 25.5%, 11.8% and 9.8% of isolates, respectively.Conclusions results show the importance of asp determination in veterinary isolates and the need for using standardized and validated testing methods and harmonized interpretive criteria.

Journal: Veterinary sciences (2022)
Publication types: Journal Article
Pycnogenol® supplementation alleviates symptoms of Parkinson's disease with mild cognitive impairment.

The aim of this pilot registry study was to assess the preventive effect of an 8-week pycnogenol®(french maritime pine bark extract) supplementation on symptoms, such as cognitive and motor aspects, in subjects with parkinson's disease (pd).Methods the study was based on five types of observations: a.Karnofsky performance scale index; b.Oxidative stress markers in plasma (plasma free radicals, pfr); c.The main signs and symptoms, evaluated by a physician; d.Face motion and expressions and their symmetry, recorded with a high-speed video camera.Results a total of 79 patients were included in the study: 39 controls using only standard management (sm) and 40 subjects supplemented with pycnogenol®150 mg/day.At inclusion, the two groups (sm and sm+pycnogenol®) were comparable for age, symptoms, karnofsky performance scale rating and for the management of pd.Likewise, oxidative stress and the presence of peripheral edema were also comparable between the two groups.No side effects of supplementation were observed.There were no tolerability problems.At the end of the study after 8 weeks, the karnofsky performance index was significantly (p<0.05) higher with pycnogenol®compared to controls.Also, the proportion of patients with a clinically visible peripheral edema decreased significantly over 8 weeks with pycnogenol®compared to controls (p<0.05).Plasma oxidative stress was significantly lower with pycnogenol®(p<0.05).The scores of pd main signs/symptoms like tremor, bradykinesia, alterations in cognitive functions, rigidity and speech impairment were significantly lower in the pycnogenol®group compared to controls (p<0.05).Face expression evaluation showed a marked asymmetry at inclusion.Over 8-week supplementation, facial expression scores for visible and subliminal asymmetry, altered facial responses, altered shoulder motion and altered emotional pattern improved significantly in the pycnogenol®group compared to controls (p<0.05) where the scores did not change significantly.Conclusions in conclusion, according to this pilot registry study, pycnogenol®supplementation helps patients with moderate, well-controlled pd - under stable treatment - to attenuate most signs and symptoms and life-relational aspects associated with parkinson-related cognitive impairment.

Journal: Journal of neurosurgical sciences (2022)
Publication types: Journal Article
Distinct Activities of Gli1 and Gli2 in the Absence of Ift88 and the Primary Cilia.

The primary cilia play essential roles in hh-dependent gli2 activation and gli3 proteolytic processing in mammals.However, the roles of the cilia in gli1 activation remain unresolved due to the loss of gli1transcription in cilia mutant embryos, and the inability to address this question by overexpression in cultured cells.Here, we address the roles of the cilia in gli1 activation by expressing gli1from the gli2locus in mouse embryos.We find that the maximal activation of gli1 depends on the cilia, but partial activation of gli1 by smo-mediated hh signaling exists in the absence of the cilia.Combined with reduced gli3 repressors, this partial activation of gli1 leads to dorsal expansion of v3 interneuron and motor neuron domains in the absence of the cilia.Moreover, expressing gli1from the gli2locus in the presence of reduced sufu has no recognizable impact on neural tube patterning, suggesting an imbalance between the dosages of gli and sufu does not explain the extra gli1 activity.Finally, a non-ciliary gli2 variant present at a higher level than gli1 when expressed from the gli2locus fails to activate hh pathway ectopically in the absence of the cilia, suggesting that increased protein level is unlikely the major factor underlying the ectopic activation of hh signaling by gli1 in the absence of the cilia.

Journal: Journal of developmental biology (2019)
Publication types: Journal Article
Identification of key miRNAs and targeted genes involved in the progression of oral squamous cell carcinoma.

/purpose oral squamous cell carcinoma (oscc) is one of the most common types of head and neck squamous cell carcinoma.Accurate biomarkers are needed for early diagnosis and prognosis of oscc.Micro rnas (mi rnas) have shown great values in different types of cancers including oscc.However, most of the mi rnas involved in the development of oscc remain uncovered.This study aimed to identify hub mi rnas and m rnas in oscc.Materials and methods we explored the roles of key mi rnas, target genes and their relationships in oscc using an integrated bioinformatics approach.Initially, two oscc microarray datasets from the gene expression omnibus database were obtained to analyze mi rna expression.Mi rna-targeted m rnas were acquired, and gene ontology/kyoto encyclopedia of genes and genomes analyses were performed.Thereafter, we constructed a protein-protein interaction (ppi) network to identify hub genes and a mi rna-m rna interaction network was used to identify key mi rnas.Furthermore, differential gene expression and kaplan-meier plotter survival analysis was performed to evaluate their potential clinical application values.Results four upregulated, two downregulated mi rnas and 608 target genes of the differentially expressed mi rnas were identified.The ppi and mi rna-m rna interaction networks highlighted 10 hub genes and two key mi rnas, and pathway analyses showed their correlative involvement in tumorigenesis-related processes.Of these mi rnas and genes, mi r-125b, β-actin, vinculin and histone deacetylase 1 were correlated with overall survival (p < 0.05).Conclusion these findings indicate that mi r-21 and mi r-125b, associated with the 10 hub genes, jointly participate in oscc tumorigenesis, offering insight into the molecular mechanisms underlying oscc as potential targets for early diagnosis, treatment and prognosis.

Journal: Journal of dental sciences (2022)
Publication types: Journal Article
A Case Study of Using Telehealth in a Rural Healthcare Facility to Expand Services and Protect the Health and Safety of Patients and Staff.

This case study aimed to gain an understanding of the implementation and usage of a telehealth program during the covid-19 pandemic at a rural healthcare facility.An action research methodology, utilizing cycles of planning, implementation, review and adaptation was adopted to improve use of telehealth as covid-19 cases continued to increase.Data was collected from literature review, examination of existing documents, review of gap and swot analysis, and examination of staffing plans.This helped in ensuring that adequate resources were in place to start and continue usage of telehealth.Additionally, review of the entire process was conducted as the program advanced through various phases of implementation.By conducting rigorous analysis and reflection, these data informed cycles of improvement in the telehealth program.Challenges surrounding the continuation and usage of telehealth have also been described.Because there is a paucity of research on the use of telehealth programs in rural healthcare facilities, especially during the pandemic, this study can provide practical tips to leaders and healthcare managers.

Journal: Healthcare (Basel, Switzerland) (2021)
Publication types: Journal Article