syedzayyan/paper_mill_benchmark huggingface dataset:
Inverse Lindley power series distributions: a new compounding family and regression model with censored data.

This paper introduces a new class of distributions by compounding the inverse lindley distribution and power series distributions which is called compound inverse lindley power series (cilps) distributions.An important feature of this distribution is that the lifetime of the component associated with a particular risk is not observable, rather only the minimum lifetime value among all risks is observable.Further, these distributions exhibit an unimodal failure rate.Various properties of the distribution are derived.Besides, two special models of the new family are investigated.The model parameters of the two sub-models of the new family are obtained by the methods of maximum likelihood, least square, weighted least square and maximum product of spacing and compared them using the monte carlo simulation study.Besides, the log compound inverse lindley regression model for censored data is proposed.Three real data sets are analyzed to illustrate the flexibility and importance of the proposed models.

Journal: Journal of applied statistics (2022)
Publication types: Journal Article
Highly Soluble and Stable, High Release Rate Nanocellulose Codrug Delivery System of Curcumin and AuNPs for Dual Chemo-Photothermal Therapy.

As a natural antitumor drug, curcumin (cur) has received increasing attention from researchers and patients due to its various medicinal properties.However, currently cur is still restricted due to its low and stand-alone therapeutic effects that seriously limit its clinical application.Here, by using cellulose nanocrystals (cncs) as a nanocarrier to load cur and au nps simultaneously, we developed a hybrid nanoparticle as a codrug delivery system to enhance the low and stand-alone therapeutic effects of cur.Aided with the encapsulation of β-cyclodextrin (βcd), both the solubility and the stability of cur are greatly enhanced (solubility increased from 0.89 to 131.7 μg/m l).Owing to the unique rod-like morphology of cncs, the system exhibits an outstanding loading capacity of 31.4 μg/mg.Under the heat effects of coloaded au nps, the system demonstrates a high release rate of 77.63%.Finally, with cnc as a bridge nanocarrier, all aforementioned functions were integrated into one hybrid nanoparticle.The all-in-one integration ensures cur to have enhanced therapeutic effects and enables the delivery system to exhibit combined chemo-photothermal therapy outcomes.This work presents a significant step toward cur's clinical application and provides a new strategy for effective and integrative treatment of tumor disease.

Journal: Biomacromolecules (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Spectroscopy and DFT Calculations of Flavo-Diiron Nitric Oxide Reductase Identify Bridging Structures of NO-Coordinated Diiron Intermediates.

Flavo-diiron proteins (fdps) are widespread in anaerobic bacteria, archaea, and protozoa, where they serve as the terminal components of dioxygen and nitric oxide reductive scavenging pathways.Fdps contain an n,o-ligated diiron site adjacent to a flavin mononucleotide (fmn) cofactor.The diiron site is structurally similar to those in hemerythrin, ribonucleotide reductase, and methane monooxygenase.However, only fdps turn over no to n2o at significant rates and yields.Previous studies revealed sequential binding of two no molecules to the diferrous site, forming mono- and dinitrosyl intermediates leading to n2o formation.In the present work, these mono- and dinitrosyl intermediates have been characterized by epr and mössbauer spectroscopies and dft calculations.Our results show that the iron proximal to the cofactor binds the first no to form the diiron mononitrosyl complex, implying the iron distal to the fmn binds the second no to form the diiron dinitrosyl intermediate.The exchange-coupling constants, j (h = js1·s2), were found to differ substantially, +17 cm-1for the diiron mononitrosyl and +60 cm-1for the diiron dinitrosyl.Notwithstanding this large difference, our findings indicate retention of at least one hydroxo bridge throughout the nor catalytic cycle.The mossbauer hyperfine parameters and dft calculations confirmed a semibridging no-ligand in the mononitrosyl intermediate that lowers the exchange parameter.The dft calculations on the dinitrosyl intermediate suggest a contribution to j from direct exchange between the s = 1 spins on the no-ligands, which could initiate n-n bond formation.Our results provide insight into why fdps are the only known nonheme diiron enzymes that competently turn over no to n2o.

Journal: ACS catalysis (2018)
Publication types: Journal Article
A simple approach for random genomic insertion-deletions using ambiguous sequences in Escherichia coli.

Escherichia coli k-12, being one of the best understood and thoroughly analyzed organisms, is the preferred platform for genetic and biochemical research.Among all genetic engineering approaches applied on e.Coli, the homologous recombination approach is versatile and precise, which allows engineering genes or large segments of the chromosome directly by using polymerase chain reaction (pcr) products or synthetic oligonucleotides.The previously explained approaches for random insertion and deletions were reported as technically not easy and laborious.This study, first, finds the minimum length of homology extension that is efficient and accurate for homologous recombination, as 30 nt.Second, proposes an approach utilizing pcr products flanking ambiguous nnn-sequence (30-nt) extensions, which facilitate the homologous recombination to recombine them at multiple regions on the genome and generate insertion-deletion mutations.Further analysis found that these mutations were varying in number, that is, multiple genomic regions were deleted.Moreover, evaluation of the phenotype of all the multiple random insertion-deletion mutants demonstrated no significant changes in the normal metabolism of bacteria.This study not only presents the efficiency of ambiguous sequences in making random deletion mutations, but also demonstrates their further applicability in genomics.

Journal: Journal of basic microbiology (2022)
Publication types: Journal Article
Successful Single-kidney Transplantation in Adult Recipients Using Pediatric Donors Aged 8 to 36 Months: Comparable Outcomes With Those Using Pediatric Donors Aged >3 Years.

Using pediatric donors for single-kidney transplantation (skt) can increase the number of possible recipients.However, it is unclear when skt involving small pediatric donors and adult recipients can safely be performed without compromising graft outcome.Methods from 2013 to 2017, a total of 102 skts in adult recipients were performed in our center using pediatric donors aged <12 years.We compared the outcomes from donors aged 8 to 36 months (the small-kidney group [skg], n = 46) and from donors aged 3 to 12 years (the big-kidney group [bkg], n = 56).The median follow-up time was 30 months in the skg and 28 months in the bkg.Results all patients achieved satisfactory renal function after transplantation, despite the fact that some patients (skg, 19.6%; bkg, 28.6%) developed delayed graft function.One-year graft survival and death-censored graft survival in the skg were 89.1% and 100%, respectively, comparable to the results in the bkg (92.9% and 98.2%).One year later, the graft and patient survival rates in both groups remained unchanged.Pulmonary infection was the main cause of death in patients with a functioning graft (skg, 4 patients; bkg, 2 patients).Proteinuria occurred early in some patients (skg, 30.4%; bkg, 19.6%) and decreased gradually within the first year posttransplantation.Conclusions skt from pediatric donors aged 8 to 36 months to selected adult recipients produced excellent intermediate-term outcomes, comparable with those when older pediatric donors were used.This study provides evidence to support a lower age limit for skt from pediatric donors.

Journal: Transplantation (2019)
Publication types: Comparative Study;Journal Article;Research Support, Non-U.S. Gov't
Itraconazole for Topical Treatment of Skin Carcinogenesis: Efficacy Enhancement by Lipid Nanocapsule Formulations.

Itraconazole (itc), an antifungal drug with anticancer activity, shows potential for oral treatment of skin cancer.There is clinical need for topical itc for treating low-risk skin carcinogenesis.Our objective was to develop itc nanoformulations with enhanced anticancer efficacy.Lipid nanocapsules (lnc), either unmodified (itc/lnc) or modified with the amphiphiles miltefosine (itc/mf-lnc) or the lipopeptide biosurfactant surfactin (itc/sf-lnc) as bioactive additives were developed.Lnc formulations showed high itc entrapment efficiency (>98%), small diameter (42-45 nm) and sustained itc release.Cytotoxicity studies using malignant scc 9 cells and normal human fibroblasts (nhf) demonstrated significant enhancement of itc anticancer activity and selectivity for cancer cells by the lnc formulations and a synergistic itc-amphiphile interaction improving the combination performance.Treatment of intradermal tumor-bearing mice with the itc nanoformulation gels compared with itc and 5-fu gels achieved significant tumor growth inhibition that was remarkably enhanced by itc/mf-lnc and itc/sf-lnc as well as recovery of skin architecture.Molecularly, tumoral expression of ki-67 and cytokeratin proliferative proteins was significantly suppressed by lnc formulations, the suppressive effect on cytokeratins was superior to that of 5-fu.These findings provide new evidence for effective topical treatment of low-risk skin carcinogenesis utilizing multiple approaches that involve drug repurposing, nanotechnology, and bioactive amphiphiles as formulation enhancing additives.

Journal: Journal of biomedical nanotechnology (2022)
Publication types: Journal Article
Long-Term Observation of Higher-Order Aberrations and Microdistortions in Bowman's Layer After Small Incision Lenticule Extraction for the Correcting Myopia With Spherical Equivalent Higher Than -9.0 Diopters.

To evaluate the outcomes in corneal higher-order aberrations (hoas) and microdistortions in the bowman's layer after femtosecond laser small incision lenticule extraction (smile) for correcting extremely high myopia.Methods this prospective study included patients with myopia with spherical equivalent ≥ -9.0 diopters (d).Smile was performed in forty eyes of 40 patients.Pentacam was used to evaluate hoas before and at 1 day, 3 months, 6 months, and 2 years after surgery.Fourier-domain optical coherence tomography was used to evaluate microdistortions at 2 years postoperatively.Thirty-two eyes of 32 patients receiving femtosecond laser-assisted in situ keratomileusis (fs-lasik) were enrolled as the control group.Hoas were measured before, at 1 day and at least 1 year postoperatively.Results after smile, the long-term safety and effectiveness index was 1.25 and 0.85, respectively.Microdistortions were observed in 73.5% of the eyes at 2 years, with an average number of 1.20 ± 1.22 microdistortions and an average width of 287.37 ± 259.00 μm.We detected more microdistortions in the horizontal meridian than in the vertical meridian (p= 0.035).The average number and width of microdistortions were both higher in the central region (≤4 mm) than in the peripheral region (4-8 mm) (bothp 6.0 mm) (allp< 0.05).The increase in sa and most trefoil correlated with the mean number of central microdistortions number (allp< 0.05).Conclusion for myopia over -9.0d, the microdistortions in the bowman's layer were still detectable in most eyes long-term after smile.Both smile and fs-lasik induced more hoas, mainly hoa, sa, and coma.The small optical zone and microdistortions may affect postoperative aberrations.

Journal: Frontiers in medicine (2022)
Publication types: Journal Article
Microorganisms in Superficial Deposits on the Stone Monuments in Saint Petersburg.

The composition of superficial deposits in urban environment and their importance in the development of the lithobiotic community of microorganisms has been investigated.Polyols, organic acids, mono- and disaccharides, as well as some amino acids, are the predominant low molecular weight organic components in superficial deposits, although the conditions on the stone surface are undoubtedly oligotrophic.Superficial deposits accumulate heavy metals, including fe, mn, zn, cu, pb, and cd, in surface sediments, among which the potentially toxic elements zn, cu, and pb are accumulated in rather high concentrations.On model of aspergillus nigeras an example, it was shown micromycetes are resistant to heavy metals and retain their physiological activity when grown on this substrate.According to cultural studies, as well as metagenomic analysis, stress-resistant fungi and dark organotrophic bacteria are the main inhabitants of surface sediments.Probably, in the conditions of accumulation of superficial deposits on the stone, these organisms are the main inhabitants of the surface of the stone.With the development of more multi-species lithobiotic communities, they form the core of these communities.In the urban environment this type of primary colonization of the stone is likely realized.

Journal: Microorganisms (2022)
Publication types: Journal Article
Comparing the attitudes of physicians and non-physicians toward communicating a patient's BRCA1 mutation to a first-degree relative against a patient's wishes.

Although genetic testing can be vastly informative, it creates a dilemma if a patient does not want to disclose an abnormal genetic test to at-risk relatives.A sample of 200 participants from nevada (100 physicians, 100 non-physicians) completed an 11-item questionnaire asking demographic information, familiarity with genetics and genetic testing, and opinions about a physician's role in a hypothetical case in which a patient does not wish to communicate her brca1 mutation to her sister.Although most respondents did not think the physician should notify the sister against the patient's wishes, more non-physicians (40%) than physicians (23%) contended that the physician should do so (p = 0.0119).Most respondents from both groups agreed that the physician should not have the legal duty to notify the sister, would not be morally justified in sharing genetic test results with the sister, but should have the right to notify a patient's relatives if the disease is "serious, preventable, and treatable." more non-physicians than physicians agreed that physicians should have an educational requirement on how to communicate genetic test results to patients and their family (88% vs 65%, p = 0.0002).Most physicians (70%) reported a familiarity/strong familiarity with genetic testing compared to non-physicians (33%; p < 0.0001).Future qualitative research should assess physicians' understanding of issues surrounding familial communication of genetic test results.Educational interventions to facilitate effective communication to patients and families are needed and welcomed by most physicians.Discrepancies between the attitudes of physicians and patients or the public need to be better understood and addressed.

Journal: Journal of community genetics (2022)
Publication types: Journal Article
The genetic basis of transpiration sensitivity to vapor pressure deficit in wheat.

Genetic manipulation of whole-plant transpiration rate (tr) response to increasing atmospheric vapor pressure deficit (vpd) is a promising approach for crop adaptation to various drought regimes under current and future climates.Genotypes with a non-linear tr response to vpd are expected to achieve yield gains under terminal drought, thanks to a water conservation strategy, while those with a linear response exhibit a consumptive strategy that is more adequate for well-watered or transient-drought environments.In wheat, previous efforts indicated that tr has a genetic basis under naturally fluctuating conditions, but because tr is responsive to variation in temperature, photosynthetically active radiation, and evaporative demand, the genetic basis of its response vpd per se has never been isolated.To address this, we developed a controlled-environment gravimetric phenotyping approach where we imposed vpd regimes independent from other confounding environmental variables.We screened three nested association mapping populations totaling 150 lines, three times over a 3-year period.The resulting dataset, based on phenotyping nearly 1400 plants, enabled constructing 63-point response curves for each genotype, which were subjected to a genome-wide association study.The analysis revealed a hotspot for tr response to vpd on chromosome 5a, with snps explaining up to 17% of the phenotypic variance.The key snps were found in haploblocks that are enriched in membrane-associated genes, consistent with the hypothesized physiological determinants of the trait.These results indicate a promising potential for identifying new alleles and designing next-gen wheat cultivars that are better adapted to current and future drought regimes.

Journal: Physiologia plantarum (2022)
Publication types: Journal Article
The emerging role of furin in neurodegenerative and neuropsychiatric diseases.

Furin is an important mammalian proprotein convertase that catalyzes the proteolytic maturation of a variety of prohormones and proproteins in the secretory pathway.In the brain, the substrates of furin include the proproteins of growth factors, receptors and enzymes.Emerging evidence, such as reduced furin m rna expression in the brains of alzheimer's disease patients or schizophrenia patients, has implicated a crucial role of furin in the pathophysiology of neurodegenerative and neuropsychiatric diseases.Currently, compared to cancer and infectious diseases, the aberrant expression of furin and its pharmaceutical potentials in neurological diseases remain poorly understood.In this article, we provide an overview on the physiological roles of furin and its substrates in the brain, summarize the deregulation of furin expression and its effects in neurodegenerative and neuropsychiatric disorders, and discuss the implications and current approaches that target furin for therapeutic interventions.This review may expedite future studies to clarify the molecular mechanisms of furin deregulation and involvement in the pathogenesis of neurodegenerative and neuropsychiatric diseases, and to develop new diagnosis and treatment strategies for these diseases.

Journal: Translational neurodegeneration (2022)
Publication types: Journal Article;Review;Research Support, Non-U.S. Gov't
Consolidated Overview of Notifiable Adverse Events in the U.S. President's Emergency Plan for AIDS Relief's Voluntary Medical Male Circumcision Program Through 2020.

Of review through december 2020, the u.s.President's emergency plan for aids relief (pepfar) supported more than 25 million voluntary medical male circumcisions (vmmc) as part of the combined hiv prevention strategy in 15 african countries.Pepfar monitors defined adverse events (aes) occurring within 30 days of vmmc through its notifiable adverse event reporting system (naers).All naers reports through december 2020 were reviewed to quantify ae type, severity, and relation to the vmmc procedure.Interventions to improve client safety based on naers findings are described.Recent findings fourteen countries reported 446 clients with notifiable adverse events (naes); 394/446 (88%) were determined vmmc-related, representing approximately 18 nae reports per million circumcisions.Fatalities comprised 56/446 (13%) with 24/56 (43%) of fatalities determined vmmc-related, representing 0.96 vmmc-related fatalities per million circumcisions.The remaining 390 naes were non-fatal with 370/390 (95%) vmmc-related.Multiple programmatic changes have been made based on naers data to improve client safety.Client safety is paramount in this surgical program designed for individual and population-level benefit.Surveillance of rare but severe complications following circumcision has identified pre-existing or new safety concerns and guided continuous programmatic improvement.

Journal: Current HIV/AIDS reports (2022)
Publication types: Journal Article;Review
The contribution of personal audio system use and commuting by bus on daily noise dose.

For many young people, exposure to music from personal audio system use may represent a significant component of daily noise dose.Moreover, there is increasing concern for the hearing of those who listen at high volumes.The purpose of this study was to determine the noise levels experienced on commuter buses, and to investigate how these impact on the volume-setting behavior of young adult personal audio system users.Methods a questionnaire was used to probe transport use, personal audio system-listening behaviors and the extent of understanding about noise-induced hearing loss.The influence of bus noise on volume-setting behavior was determined by measuring, in a lab setting, the sound-level preferences of participants when listening to their favorite song, a generic song, or a podcast in the absence and presence of various levels of bus noise, simulated using output-adjusted recordings made of bus noise.Statistical analysis was conducted using analysis of variance.Results while the bus noise itself was below 85 d b leq, as the sound level of the buses increased, so did the percentage of commuters who were found to exceed the equivalent of 8 hours of exposure at 85 d b leq.Implications investment in buses with lower noise levels or the use of noise-canceling or noise-occluding headphones would help to reduce the likelihood of noise-induced hearing loss for bus commuters.

Journal: Noise & health (2021)
Publication types: Journal Article
Dietary isoflavones intake is inversely associated with non-alcoholic fatty liver disease, hyperlipidaemia and hypertension.

This study investigated associations between total isoflavones and their categories (daidzein, genistein, glycitein) intake and the risks for metabolic disorders.We used the data of 6786 chinese adults from the nutrition health atlas project.We performed multiple logistic regression and restricted cubic spline models assessing the risks for metabolic disorders (non-alcoholic fatty liver disease (nafld), hyperlipidaemia, hypertension, diabetes and overweight/obesity) in each category of isoflavones.Higher total isoflavones, daidzein and genistein intake were inversely associated with nafld (p< .05).Higher total isoflavones, daidzein, genistein and glycitein intake were also inversely associated with hyperlipidaemia (p< .01) and hypertension (p< .01).Dose-response analyses revealed that total isoflavones, daidzein, genistein and glycitein intakes were associated with the risks of metabolic disorders in a nonlinear trend.In conclusion, total isoflavones, daidzein and genistein intake were inversely associated with nafld, hyperlipidaemia and hypertension.Glycitein was inversely associated with hyperlipidaemia and hypertension.

Journal: International journal of food sciences and nutrition (2022)
Publication types: Journal Article