syedzayyan/paper_mill_benchmark huggingface dataset:
Degradation and in-use stability study of five marketed therapeutic monoclonal antibodies by generic weak cation exchange liquid chromatographic method ((WCX)HPLC/DAD).

Therapeutic monoclonal antibodies (m abs) represent a very important class of the current biopharmaceutics.The great complexity of their structure made necessary the use of different analytical approaches for assessing different physico-chemical properties.In this work, weak cation exchange (wcx) high performance liquid chromatography with diode array detection ((wcx)hplc/dad) is used to assess the charge variant profile.The method here developed combined the effect of ionic strength and controlled p h gradient and allows for the charge variants analysis of the five m abs studied, namely bevacizumab (bvz), cetuximab (ctx) infliximab (inf), rituximab (rtx) and trastuzumab (ttz), which are among the most used m abs worldwide.The differences in the charge variants in the natural isoforms of the m abs promoted characteristic wcx chromatograms for each of m abs that can be also useful for identification purposes.These chromatograms have provided to be suitable for tracking changes in the charge variants of each m ab analyzed both in controlled degraded and in stabilities study along time of in-use samples solutions at 2 mg/m l in 0.9% na cl stored refrigerated (at 4 °c) and frozen (-20 °c) for two months.The results obtained indicated different stabilities of these m abs, all ig g1, against degradation by different stressed environmental conditions and in-use stability along two months.

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2022)
Publication types: Journal Article
Senescence of song revealed by a long-term study of the Seychelles warbler (Acrocephalus sechellensis).

Senescence is widespread in nature, often resulting in diminishing survival or reproduction with age, but its role in age-dependent variation in sexual traits is often poorly understood.One reason is that few studies of sexual traits consider non-linear relationships with age, or only consider a narrow range of years relative to the life span of the species.Birdsong has evolved to allow assessment of conspecific quality in numerous bird species.Whilst theory and empirical work suggests that song may become more elaborate with age, there are a paucity of long-term studies testing whether song is associated with age or longevity.In particular, the occurrence of song senescence has rarely been demonstrated.Using an exceptional long-term dataset for the seychelles warbler (acrocephalus sechellensis), we analysed relationships between male song, age, survival, and longevity.This species is a long-lived songbird with early life increases, followed by senescent declines, in survival and reproduction.The study population (cousin island, seychelles) is a closed population, with no depredation of adults, providing an excellent opportunity to study senescence in free-living animals.We tested whether song traits were related to age at recording, future survival, longevity, and territory quality.We found age-dependent changes in five song traits (duration, maximum frequency, peak frequency of songs, and duration and frequency bandwidth of trills).Relationships with age were quadratic, indicating reversal in the expression of song coinciding with the onset of senescence in reproduction and survival in this species.One song trait (trill bandwidth) had a quadratic relationship with future survival, but no song traits were related to longevity, suggesting age-related patterns were not the result of selective disappearance.Our study provides one of the first examples of functional senescence in song, offering new insights into avian senescence.Late-life declines in avian song, and possibly other sexual traits, may be more common than currently known, and may play a fundamental role in age-dependent changes in reproductive success.

Journal: Scientific reports (2020)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Sweet Immune Checkpoint Targets to Enhance T Cell Therapy.

Despite tremendous success against hematological malignancies, the performance of chimeric ag receptor t cells against solid tumors remains poor.In such settings, the lack of success of this groundbreaking immunotherapy is in part mediated by ligand engagement of immune checkpoint molecules on the surface of t cells in the tumor microenvironment.Although ctla-4 and programmed death-1 (pd-1) are well-established checkpoints that inhibit t cell activity, the engagement of glycans and glycan-binding proteins are a growing area of interest due to their immunomodulatory effects.This review discusses exemplary strategies to neutralize checkpoint molecules through an in-depth overview of genetic engineering approaches aimed at overcoming the inhibitory programmed death ligand-1 (pd-l1)/pd-1 axis in t cell therapies and summarizes current knowledge on glycoimmune interactions that mediate t cell immunosuppression.

Journal: Journal of immunology (Baltimore, Md. : 1950) (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't;Research Support, U.S. Gov't, Non-P.H.S.;Review
Relationship between Gestational Weight Gain Rate Trajectory and Overweight in Offspring at Three Years of Age.

The incidence of childhood obesity has increased rapidly, and its relationship with adult diseases is constantly being revealed.Maternal factors have been shown to play an important role in the growth and development of newborns.In this article, we explored the relationship between the gestational weight gain (gwg) rate and overweight/obesity in offspring at 3 years of age.methods:a total of 5146 pregnant women and their children registered between january 2010 and december 2018 were studied by a retrospective cohort study.The group-based trajectory model was used to distinguish the gwg rate patterns.Overweight/obesity was diagnosed by the weight-for-height z-score at 3 years of age.Logistic regression was used to analyze the association between gwg rate patterns and outcomes.Odds ratio (or) and 95% confidence interval (95% ci) were calculated to analyze the association between gwg rate patterns and overweight/obesity in offspring at 3 years of age.results:three gwg rate growth patterns were identified in this study.The incidence of offspring overweight/obesity in the low-stable, high-stable, and low-sharp patterns was 8.33%, 3.68%, and 6.03% respectively.After adjusting covariates, compared with the low-stable pattern, the high-stable pattern increased the risk for offspring to be overweight/obesity at 3 years of age, with or of 2.26 (95% ci, 1.31-3.90).However, the low-sharp pattern was not associated with overweight/obesity in offspring at 3 years of age.conclusions:the high-stable increasing pattern of the gwg rate is a risk factor for overweight/obesity in offspring at 3 years of age.

Journal: Childhood obesity (Print) (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
How to address cerebrospinal fluid leakage following ossification of the posterior longitudinal ligament surgery.

Primary dural repair in ossification of posterior longitudinal ligament (opll) surgery via anterior is challenging because of limited space.Based on several years of our experiences, we present our concept for addressing cerebrospinal fluid (csf) leakage following dural tear during anterior opll surgery.Methods we reviewed the medical records of 65 patients who underwent anterior opll surgery from 2003 to 2014.We included 7 patients whose operation records described dural tear followed by csf leakage.Primary dural repair could not be performed in 6 patients because of ragged tear and limited space.We managed them with our own strategy: simple cover with an artificial dura, collagen sponge and/or fibrin glue on defect site; anatomical layer-by-layer repair without either subfascial or csf drain, and early ambulation.Results of 7 patients, wound problems developed in 2 patients.Wound aspiration and antibiotic treatment was done without wound compression in one patient, and simple irrigation followed by anatomical layer-by-layer re-closure was done in the other patient without either bed rest or lumbar drainage.Pseudomeningoceles were detected in 4 patients, including 2 patients who had wound problems.However, all pseudomeningoceles were absorbed spontaneously or stabilized on follow-up images without interventions such as bed rest, lumbar drain or wound compression.Conclusions without interference of pressure equilibrium between intradural and extradural space, the defect site would heal and close.After that, the isolated pseudomeningocele would be spontaneously absorbed after certain period of time.

Journal: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia (2017)
Publication types: Journal Article
Association Study of Alcohol Dehydrogenase and Aldehyde Dehydrogenase Polymorphism With Alzheimer Disease in the Taiwanese Population.

Alcohol dehydrogenase (adh) and aldehyde dehydrogenase (aldh) are two major alcohol-metabolizing enzymes.Moderate alcohol intake is a protective modified factor in alzheimer's disease (ad) while heavy alcohol intake and abstinence increased dementia risk.The associations between alzheimer's disease and alcohol-metabolizing genes are uncertain.This study examined the association of ad with seven adh/aldhsingle-nucleotide polymorphisms (snps),adh1crs2241894,adh1brs1229984,aldh1b1rs2073478,aldh2rs886205, rs4767944, rs4648328, and rs671.We enrolled 157 ad and 168 age- and sex-matched control subjects in pilot study to examine the association of ad with adh/aldhsnps.Reconstructed aldh2haplotypes were performed.We measured plasma level of adh1c and checked the interaction effect of ad-rs2241894 genotype on plasma adh1c level.In extension study, we further examined 339 ad and 2,504 healthy control from the taiwan biobank.In pilot study, we observed that adh1crs2241894 tt genotype was negatively associated with ad in a recessive genetic model (or = 0.25, 95% ci 0.09-0.75,p< 0.0001) in women.A strong linkage disequilibrium was observed among the four examined snps of aldh2.No haplotype was related to ad.The plasma adh1c level in ad was higher than that in control.After adjusted by age, sex, hypertension, diabetes mellitus, and alcohol, we found a significant interaction effect of ad-rs2241894 genotype on plasma adh1c level (p= 0.04).This interaction effect was attributable to the association between ad and plasma adh1c level (β estimate = 366, 95% ci 92.7∼639.4,p= 0.009).The genetic distribution of adh1crs2241894 showed strong ethnic heterogeneity, in which the t allele was the minor allele accounting for 28.5% in our study and 23.6% in east asians, while it was a major allele in americans, europeans, and the global populations.No association was discovered between ad and the five snps: rs2241894, rs1229984, rs2073478, rs886205, and rs671 in the extension study.In summary, this study revealed a suggestive association between adh1c rs2241894 and female ad in the pilot study, but failed to confirm this finding in a population database.Further age-matched and large sample size case-control studies are needed before rs2241894 can be interpreted as a protective genetic factor of ad.

Journal: Frontiers in neuroscience (2021)
Publication types: Journal Article
Stearic acid mediated growth of edge-on oriented bilayer poly(3-hexylthiophene) Langmuir films.

The growth and structural evolution of stearic acid (sa) blended poly(3-hexylthiophene) [p3ht] langmuir and langmuir-blodgett (lb) films were studied using complimentary surface and interface sensitive techniques to understand the possibility of ordering and layering of promising charge carrier mobility polymers, at the air-water interface and on the transferred solid substrate.Sa-induced and subsequent compression-induced transitions in p3ht structure, from aggregated-3d to soft-2d and from in-plane mixed to unmixed layer, are evident at low and high pressures, respectively.The blending of sa molecules enhances the amphiphilic character of p3ht, which reduces the extent of the out-of-plane aggregation to form edge-on oriented (eo) bottom side-chain folded-bilayer (f-bl) islands (of size ~60 nm) within sa monolayer (ml), of commensurate thickness (~2.6 nm).Further compression, gradually rejects the less hydrophilic f-bl islands from the mixed layer to form eo p3ht bl islands (of coverage in-tune with starting composition) on top of sa ml.The formation of nearly covered p3ht(bl)/sa(ml) structured film on solid substrate is evident for the first time, which (even of limited p3ht thickness) has immense importance in the device properties, as the current in the bottom-gated organic thin-film transistors is known to travel only within few ml region near gate-dielectric.

Journal: Journal of colloid and interface science (2022)
Publication types: Journal Article
Possible reasons for the regrettable results of patency of an inside stent in endoscopic transpapillary biliary stenting.

Endoscopic biliary decompression is a minimally invasive procedure for cholestasis since the first endoscopic retrograde cholangiopancreatography-guided biliary stenting performed by soehendra and reynders-frederix.Among the endoscopic biliary decompression, endoscopic transpapillary biliary stenting (ebs), is a mainstream choice and presently has two methods of placement: stenting above the sphincter of oddi (so) (suprapapillary) and stenting across the so (transpapillary).Stent patency is the most important concern for patients, endoscopists and physicians because it can affect both the life prognosis and treatment schedule of patients.Biliary stent occlusion can occur because of several factors.Among them, direct food impaction, biofilm formation, and sludge formation play important roles and are presumed to be theoretically overcome by ebs above the so.Thus, ebs above the so is expected to result in a longer patency than ebs across the so.In the literature, there have been six comparative studies on ebs for distal biliary obstruction in which the stent was placed above or across the so, including two randomized controlled trials (rcts) with negative results of stenting above the so.With respect to ebs for hilar biliary obstruction, there have been no rcts, whereas four retrospective comparative studies with negative results and four retrospective comparative studies showing positive results of stenting above the so have been reported.In this review, we focused on ebs above and across the so, and summarized the positive and negative results of the two types of stenting to promote effective clinical practice and to provide a basis for future studies.

Journal: Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society (2022)
Publication types: Journal Article;Review
Design of Compact and Broadband Polarization Beam Splitters Based on Surface Plasmonic Resonance in Photonic Crystal Fibers.

In this work, a polarization beam splitter (pbs) based on surface plasmonic resonance is proposed and realized in a designed photonic crystal fiber (pcf).The pcf consists of two kinds of air holes with different diameters.Two solid silica cores near the center of the pcf are established by removing the cladding air holes.A gold film is plated at the external surface of the central air hole of the pcf to excite the surface plasmonic resonance.In order to minimize the length and improve the operation bandwidth of the pbs, the influences of the transversal structural parameters of the pcf are investigated in the context of both xand ypolarization beams.It was found that a 123.6-μm-long pbs with an operation bandwidth of 314 nm could be realized after the global optimization of five structural parameters.The proposed pbs may have potential applications in micro-/nano-optical systems for sensing and communications.

Journal: Micromachines (2022)
Publication types: Journal Article
The mental health of NHS staff during the COVID-19 pandemic: two-wave Scottish cohort study.

Health and social care workers (hscws) are at risk of experiencing adverse mental health outcomes (e.g.Higher levels of anxiety and depression) because of the covid-19 pandemic.This can have a detrimental effect on quality of care, the national response to the pandemic and its aftermath.Aims a longitudinal design provided follow-up evidence on the mental health (changes in prevalence of disease over time) of nhs staff working at a remote health board in scotland during the covid-19 pandemic, and investigated the determinants of mental health outcomes over time.Method a two-wave longitudinal study was conducted from july to september 2020.Participants self-reported levels of depression (patient health questionnaire-9), anxiety (generalised anxiety disorder-7) and mental well-being (warwick-edinburgh mental well-being scale) at baseline and 1.5 months later.Results the analytic sample of 169 participants, working in community (43%) and hospital (44%) settings, reported substantial levels of depression and anxiety, and low mental well-being at baseline (depression, 30.8%; anxiety, 20.1%; well-being, 31.9%).Although mental health remained mostly constant over time, the proportion of participants meeting the threshold for anxiety increased to 27.2% at follow-up.Multivariable modelling indicated that working with, and disruption because of, covid-19 were associated with adverse mental health changes over time.Conclusions hscws working in a remote area with low covid-19 prevalence reported substantial levels of anxiety and depression, similar to those working in areas with high covid-19 prevalence.Efforts to support hscw mental health must remain a priority, and should minimise the adverse effects of working with, and disruption caused by, the covid-19 pandemic.

Journal: BJPsych open (2022)
Publication types: Journal Article
Cellular lasers for cell imaging and biosensing.

The possibility to produce laser action involving biomaterials, in particular (single) biological cells, has fostered the development of cellular lasers as a novel approach in biophotonics.In this respect, cells that are engineered to carry gain medium (e.g., fluorescent dyes or proteins) are placed inside an optical cavity (i.e., typically a sandwich of highly reflective mirrors), allowing the generation of stimulated emission upon sufficient optical pumping.In another scenario, micron-sized optical resonators supporting whispering-gallery mode (wgm) or semiconductor-based laser probes can be internalized by the cells and support light amplification.This review summarizes the recent advances in the fields of biolasers and cellular lasers, and most importantly, highlights their potential applications in the fields of in vitro and in vivo cell imaging and analysis.They include biosensing (e.g., in vitro detection of sodium chloride (na cl) concentration), cancer cell imaging, laser-emission-based microscope, cell tracking, cell distinction study, and tissue contraction monitoring in zebrafish.Lastly, several fundamental issues in developing cellular lasers including laser probe fabrication, biocompatibility of the system, and alteration of local refractive index of optical cavities due to protein absorption or probe aggregation are described.Cellular lasers are foreseen as a promising tool to study numerous biological and biophysical phenomena.Statement of significance: biolasers are generation of laser involving biological materials.Biomaterials, including single cells, can be engineered to incorporate laser probes or fluorescent proteins or fluorophores, and the resulting light emission can be coupled to optical resonator, allowing generation of cellular laser emission upon optical pumping.Unlike fluorescence, this stimulated emission is very sensitive and is capable of detecting small alterations in the optical property of the cells and their environment.In this review, recent development and applications of cellular lasers in the fields of in vitro and in vivo cell imaging, cell tracking, biosensing, and cell/tissue analysis are highlighted.Several challenges in developing cellular lasers including probe fabrication and biocompatibility as well as alteration of cellular environment are explained.

Journal: Acta biomaterialia (2022)
Publication types: Journal Article;Review;Research Support, Non-U.S. Gov't
Correlation between Traditional Chinese Medicine Symptom Patterns and the Renal Function, Immunologic Function Index, and Blood Coagulation Index in Patients with Henoch-Schönlein Purpura Nephritis.

We investigate the correlation between the patterns of traditional chinese medicine (tcm) syndromes and the damage of renal function, immunologic function index, and blood coagulation index in patients with henoch-schönlein purpura nephritis (hspn) and thus provide the therapeutic effects of chinese herbs decoction on hspn.Methods we studied 262 hospitalized patients diagnosed with hspn between 1 february 2016 and 1 january 2017.Indexes like renal function, immunologic function, and blood coagulation were measured.The patients were classified into four different patterns of tcm symptoms.Results in a total of 262 patients with hspn, dampness-heat accumulation accounted for 59.5%, which is the highest proportion of tcm symptom patterns, liver-kidney yin deficiency accounted for 17.6%, qi and yin deficiency ratio reached 12.6%, and blood-heat bleeding accounted for 9.9%.24-hour proteinuria was heavier in the dampness-heat accumulation patients who had immune disorders and were in hypercoagulative state and hyperfibrinolysis conditions.Conclusion we analyzed and summarized the clinical characteristics of patients with hspn and found that dampness-heat accumulation was dominant in patients and was always accompanied by immune disorders and coagulation disorders.These results provided the largest therapeutic effects of chinese herbs decoction for clinical treatment.

Journal: Evidence-based complementary and alternative medicine : eCAM (2018)
Publication types: Journal Article
Association of retinal vessel density with retinal sensitivity in surgery for idiopathic epiretinal membrane.

The success of surgical treatment for idiopathic epiretinal membrane (erm) is measured by postoperative best-corrected visual acuity (bcva), metamorphopsia, and foveal retinal sensitivity (rs).this study searched for predictive biomarkers of surgical success by determining the association between foveal rs and various aspects of vessel density (vd) in the fovea of patients with erm.Methods the study examined 25 eyes of 25 patients with erm who underwent 27-gauge microincision vitrectomy surgery (mivs).Rs was measured with microperimetry (mp-3; nidek) at four central points in the fovea with an interpoint distance of 2°.Vd was measured with sd-oct (rs 3000; nidek) within the 1-mm2square defined by the 4 rs points at various depths, including the superficial and deep retinal capillary plexus (scp and dcp, respectively).Results though vd did not change throughout the follow-up period, bcva and rs significantly improved 1 and 3 months after surgery, respectively (both p < 0.0017).Postoperative rs at 6 months was positively correlated with postoperative dcp vd at 1, 3, and 6 months (r = 0.62, p = 0.001; r = 0.40, p = 0.049; r = 0.53, p = 0.007, respectively), but not with scp vd at any time point.Multiple regression analysis confirmed that postoperative rs at 6 months was associated with postoperative dcp vd at 1 month (p = 0.03).Conclusion higher postoperative dcp vd at 1 month contributed to better postoperative foveal rs at 6 months.Early postoperative vd in the fovea might be a useful predictive biomarker of late postoperative rs in the fovea in erm patients.

Journal: Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie (2020)
Publication types: Journal Article;Observational Study