syedzayyan/paper_mill_benchmark huggingface dataset:
The expression of microRNA-23a regulates acute myocardial infarction in patients and in vitro through targeting PTEN.

Cardiovascular disease is responsible for one of the highest rates of fatality worldwide.The present study investigated the presence and influence of micro rna (mi rna)-23a in the regulation of acute myocardial infarction (ami).A total of 6 patients with ami and 6 normal volunteers without myocardial disease were included, and blood samples were taken to analyze the expression of mi rna‐23a by reverse transcription‐quantitative polymerase chain reaction.Mi rna‐23a expression in patients with ami was downregulated compared with the normal group.In h9c2 cells treated with h2o2, upregulation of mi rna‐23a expression increased the superoxide dismutase, glutathione and catalase activity levels, and suppressed the malonaldehyde activity level, as determined by elisa.Western blot analysis and a caspase‐3 substrate assay demonstrated that upregulation of mi rna‐23a expression suppressed the bcl‐2‐associated x (bax)/bcl‐2 protein expression ratio, caspase‐3 activity level and tumor suppressor p53 (p53) protein expression in h2o2‐induced h9c2 cells.Furthermore, downregulation of phosphatase and tensin homolog (pten), by the pten inhibitor bp v(hopic), increased mi rna‐23a expression and suppressed the bax/bcl‐2 protein expression ratio, caspase‐3 activity level and p53 protein expression in h2o2‐induced h9c2 cells.Therefore, the results of the present study indicate that the expression of mi rna‐23a may regulate ami through targeting pten in patients and in vitro, and pten/mi rna‐23a may therefore be potential targets for the clinical treatment of ami.

Journal: Molecular medicine reports (2018)
Publication types: Journal Article
Vitamin D deficiency in Bangladesh: A review of prevalence, causes and recommendations for mitigation.

Vitamin d is essential for the maintenance of calcium homeostasis and bone mineralization.Overt deficiency of vitamin d causes rickets in children and osteomalacia in adults.Vitamin d deficiency is an important public health problem worldwide.This review examines the available published data from all peer-reviewed original research articles of community and hospital-based research carried out on vitamin d status in different population groups in bangladesh.Baseline data of intervention trials are also included.The available selected articles were in english and retrieved from 2002 to january 2022.The paper concentrates on underlying factors for increased prevalence of vitamin d deficiency in bangladesh.Studies uniformly report very high prevalences of hypovitaminosis d among different population groups.Age and sex-specific comparisons indicate that prevalence is higher for the elderly and women.Hypovitaminosis d ranged from 21 to 75 % for infants, children, and adolescents, 38 to 100 percent for premenopausal women, 66 to 94.2 % for pregnant women, 6 to 91.3 % for adult men and 82 to 95.8 % for postmenopausal women.Important underlying factors related to this silent epidemic include dark skin colour, homebound and sedentariness, insufficient sunlight exposure, atmospheric pollution, clothing style, obesity, use of sunscreen and no supplementation.A comprehensive strategy to alleviate and control the health consequences of vitamin d deficiency is needed.This would include the creation of public awareness, refrain in sunscreen usage, exposure to sunlight, regular exercise, food fortification, and supplementation with vitamin d (bearing in mind potential differences between them and food-based sources).

Journal: Asia Pacific journal of clinical nutrition (2022)
Publication types: Journal Article;Review
The genetic control of polyacetylenes involved in bitterness of carrots (Daucus carota L.): Identification of QTLs and candidate genes from the plant fatty acid metabolism.

Falcarinol-type polyacetylenes (pas) such as falcarinol (fa oh) and falcarindiol (fa doh) are produced by several apiaceae vegetables such as carrot, parsnip, celeriac and parsley.They are known for numerous biological functions and contribute to the undesirable bitter off-taste of carrots and their products.Despite their interesting biological functions, the genetic basis of their structural diversity and function is widely unknown.A better understanding of the genetics of the pa levels present in carrot roots might support breeding of carrot cultivars with tailored pa levels for food production or nutraceuticals.Results a large carrot f2progeny derived from a cross of a cultivated inbred line with an inbred line derived from a daucus carota ssp.Commutatus accession rich in pas was used for linkage mapping and quantitative trait locus (qtl) analysis.Ten qtls for fa oh and fa doh levels in roots were identified in the carrot genome.Major qtls for fa oh and fa doh with high lod values of up to 40 were identified on chromosomes 4 and 9.To discover putative candidate genes from the plant fatty acid metabolism, we examined an extended version of the inventory of the carrot fatty acid desaturase2 (fad2) gene family.Additionally, we used the carrot genome sequence for a first inventory of eceriferum1 (cer1) genes possibly involved in pa biosynthesis.We identified genomic regions on different carrot chromosomes around the found qtls that contain several fad2 and cer1 genes within their 2-lod confidence intervals.With regard to the major qtls on chromosome 9 three putative cer1 decarbonylase gene models are proposed as candidate genes.Conclusion the present study increases the current knowledge on the genetics of pa accumulation in carrot roots.Our finding that carrot candidate genes from the fatty acid metabolism are significantly associated with major qtls for both major pas, will facilitate future functional gene studies and a further dissection of the genetic factors controlling pa accumulation.Characterization of such candidate genes will have a positive impact on carrot breeding programs aimed at both lowering or increasing pa concentrations in carrot roots.

Journal: BMC plant biology (2022)
Publication types: Journal Article
Long-Term Predictors of Hospitalized Reinfarction after an Incident Acute Myocardial Infarction.

The aim of this study was to compare characteristics of incident acute myocardial infarction (ami) and first and second time reinfarctions in terms of sociodemographic characteristics, comorbidities, symptoms, treatment, clinical characteristics, medication and outcome.A further aim was to identify predictors for an increased risk of hospitalized reinfarction.Between 2000 and 2017, a total of 13,276 ami cases were recorded by a population-based registry in the area of augsburg, germany, and were included in this study (11,871 incident events, 1217 cases of first-time reinfarction and 202 cases of second-time reinfarction).Median follow-up time was 5.3 years.For differences in baseline characteristics, chi-square tests and analysis of variance (anova) were calculated.To determine factors that are associated with an increased risk of hospitalized reinfarction cox regression models were fitted.Myocardial reinfarctions differ from incident events in some major characteristics such as the frequency of comorbidities, laboratory values, ecg presentation and therapy, but not regarding 28-day mortality.Moreover, typical comorbidities and risk factors (diabetes, hypertension, hyperlipidemia, smoking, impaired renal function) are associated with an increased risk of hospitalized reinfarction.Conversely, stemi ecg, being married, german nationality and bypass surgery are predictors for a lower risk of hospitalized reinfarction.Incident ami and reinfarction are distinctly different in many characteristics, which physicians should have in mind when treating patients with prior ami.Typical comorbidities are risk factors for hospitalized reinfarction.This underlines the importance of comprehensive treatment of these comorbidities including education of patients and encouragement towards lifestyle adjustments.

Journal: Life (Basel, Switzerland) (2022)
Publication types: Journal Article
NordiQC Assessments of PAX8 Immunoassays.

This paper is number 3 in a series developed through a partnership between isimm and nordi qc for the purpose of reporting research assessing the performance characteristics of immunoassays in an external proficiency testing program.

Journal: Applied immunohistochemistry & molecular morphology : AIMM (2018)
Publication types: Journal Article
Can We Satisfy Family in Intensive Care Unit? A Tunisian Experience.

Communication improvement and family satisfaction in intensive care unit (icu) are the main indicators of care quality.Our study aims were to evaluate family satisfaction in our intensive care and identify factors influencing the satisfaction level.Materials and methods we performed a descriptive prospective study in the icu of ben arous régional hospital conducted between october 2016 and june 2018.We included parents of patients hospitalized for more than 48 hours, with available contact details and they agreed to reply to the questionnaire.Results one hundred and twelve family representatives were included.Ten (9%) were illiterate and 40 (36%) had a primary level education.Noninvasive ventilation and hemodialysis were, respectively, used in 53 and 9.8% of cases.Thirteen patients had sequelae at their hospital discharge.The median satisfaction score was 133.5 (120; 145.7).Ninety-five (85%) relatives were always satisfied with cleanliness of the unit.The medical and paramedical staff availability was appreciated as excellent, respectively, by 65 (56%) and 66 (59%) family members.The information provided by doctors and paramedical staff was considered very clear by 75 (65%) and 65 (58%) parents, respectively.The medical secret was respected by medical (n= 107) and paramedical (n= 105) staffs in most cases.Patient management was considered excellent by 90 (80%) parents.The level of satisfaction was lower when the parent interviewed was illiterate (p= 0.04) or had a primary-level education (p= 0.012), with hemodialysis resort (p= 0.011) and with the presence of sequelae at hospital discharge (p= 0.017).Conclusion family members were satisfied with the unit environment, the communication, the healthcare management, and the patient care.Low education level, hemodialysis use, and sequelae at hospital discharge influence negatively the satisfaction.How to cite this article fathallah i, drira h, habacha s, kouraichi n.Can we satisfy family in intensive care unit?A tunisian experience.Indian j crit care med 2022;26(2):185-191.

Journal: Indian journal of critical care medicine : peer-reviewed, official publication of Indian Society of Critical Care Medicine (2022)
Publication types: Journal Article
Toward Catalytic Antibiotics: Redesign of Fluoroquinolones to Catalytically Fragment Chromosomal DNA.

A library of ciprofloxacin-nuclease conjugates was designed and synthesized to investigate their potential as catalytic antibiotics.The cu(ii) complexes of the new designer compounds (i) showed excellentin vitrohydrolytic and oxidative dnase activity, (ii) showed good antibacterial activity against both gram-negative and gram-positive bacteria, and (iii) proved to be highly potent bacterial dna gyrase inhibitors via a mechanism that involves stabilization of the fluoroquinolone-topoisomerase-dna ternary complex.Furthermore, the cu(ii) complexes of two of the new designer compounds were shown to fragment supercoiled plasmid dna into linear dna in the presence of dna gyrase, demonstrating a "proof of concept"in vitro.These ciprofloxacin-nuclease conjugates can therefore serve as models with which to develop next-generation,in vivofunctioning catalytic antimicrobials.

Journal: ACS infectious diseases (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Frontal Mucopyocoele Presenting With Recurrent Periorbital Cellulitis: A Delayed Diagnosis.

Periorbital cellulitis is an uncommon presentation to primary care and the emergency department.With multiple aetiologies, it is crucial that an appropriate history and examination are applied to identify the primary cause and initiate therapy in a timely manner.We present a 30-year-old male who presented with recurrent periorbital cellulitis treated repeatedly with antibiotics without consideration of the origin of the infection.Subsequent investigations discovered a widely dehiscent mucopyocoele of the frontal sinus that had been unrecognised and untreated.Once identified, the patient underwent functional endoscopic sinus surgery to clear the mucopyocoele and improve the drainage of the frontal sinus.Symptoms have not recurred since the surgical intervention.A poor understanding of the aetiologies of periorbital cellulitis and the related anatomy likely played a role in his delayed definitive management.Clinicians should be aware that in a patient presenting with periorbital swelling and erythema, consideration should be given to the possibility of underlying sinonasal pathology.

Journal: Cureus (2022)
Publication types: Case Reports;Journal Article
CBS gene polymorphism and promoter methylation-mediating effects on the efficacy of folate therapy in patients with hyperhomocysteinemia.

A decrease in cystathionine beta-synthase (cbs) enzyme activity could lead to hyperhomocysteinemia (hhcy).Studies have revealed that dna methylation has a mediating effect on the development of diseases.The present study aimed to explore cbs promoter methylation-mediating effects on the efficacy of folate treatment for hhcy.Methods hhcy patients were treated with folate (5 mg/day) for 90 days and then divided into a failure group (hcy ≥ 15 μmol/l) and a success group (hcy < 15 μmol/l) according to post-treatment plasma hcy levels.Genotyping of cbs gene (rs2851391 and rs706209) in patients (n = 638) was detected using a mass array system (sequenom, san diego, ca, usa).The baseline dna methylation levels of patients (n = 299) were detected using methyl targettm technology (genesky biotechnologies inc., shanghai, china).Results the cbs rs2851391 tc + cc genotype was related to a 57% reduction of failure risk in hhcy treatment compared to the tt genotype (95% confidence interval [ci] = 0.19-0.97).The cbs rs706209 ct + tt genotype had a 2.97-fold increased risk of failure to treatment compared to the cc genotype (95% ci = 1.52-5.80).After adjustment for confounding factors, the odds ratio (95% ci) for the risk of failure in hhcy treatment in total and male patients was 0.55 (0.32-0.93) and 0.34 (0.16-0.69), respectively, for patients with higher methylation levels (≥ methylation median).Additionally, baseline cbs promoter methylation mediated 33.39% of the effect of rs2851391 on the efficacy of folate treatment for hhcy (acme [average causal mediation effects]: -0.05, 95% ci = -0.11 to 0.00, p = 0.046).Conclusions the present study indicates that cbs gene polymorphism and promoter methylation could affect the efficacy of hhcy.There were potentially causal effects of genetic, epigenetic variations at the cbs rs2851391 locus on the efficacy of hhcy therapy with folate.

Journal: The journal of gene medicine (2020)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Interleaving Automatic Segmentation and Expert Opinion for Retinal Conditions.

Optical coherence tomography (oct) has become the leading diagnostic tool in modern ophthalmology.We are interested here in developing a support tool for the segmentation of retina layers.The proposed method relies on graph theory and geodesic distance.As each retina layer is characterised by different features, the proposed method interleaves various gradients during detection, such as horizontal and vertical gradients or open-closed gradients.The method was tested on a dataset of 750 oct b-scan spectralis provided by the ophthalmology department of the county emergency hospital cluj-napoca.The method has smaller signed error on layers b1, b7 and b8, with the highest value of 0.43 pixels.The average value of signed error on all layers is -1.99 ± 1.14 px.The average value for mean absolute error is 2.60 ± 0.95 px.Since the target is a support tool for the human agent, the ophthalmologist can intervene after each automatic step.Human intervention includes validation or fine tuning of the automatic segmentation.In line with design criteria advocated by explainable artificial intelligence (xai) and human-centered ai, this approach gives more control and transparency as well as more of a global perspective on the segmentation process.

Journal: Diagnostics (Basel, Switzerland) (2021)
Publication types: Journal Article
Linking Perceived Organizational Politics to Workplace Cyberbullying Perpetration: The Role of Anger and Fear.

The introduction of information and communication technologies in the workplace has extended the scope of bullying behaviors at work to the online context.However, less is known about the role of situational factors in encouraging cyberbullying behavior in the workplace.The purpose of this study is to investigate the role of perceived organizational politics in fueling cyberbullying in the workplace, and to examine the central role of negative emotions in this process.The sample comprised 279 faculty members of three large public sector universities in islamabad, pakistan.Results demonstrated that perceived organizational politics was positively associated with discrete negative emotions of anger and fear.Moreover, results indicated that anger was positively associated with cyberbullying perpetration, whereas fear was positively associated with face-to-face bullying victimization.Results also supported the idea that victims of face-to-face bullying may develop a positive attitude toward cyberbullying and retaliate against their more powerful face-to-face bullies online, possibly anonymously.We contribute to the literature by demonstrating that both forms of bullying can co-occur in the workplace as a consequence of perceived organizational politics, and the two roles-bully and victim-may be swapped among victims and perpetrators.

Journal: Journal of business ethics : JBE (2022)
Publication types: Journal Article
Perceived health and a new measure of positive youth development in adolescents with chronic conditions.

Each year, 11.8 million adolescents in the united states cope with a chronic condition (cc), yet much of the research about these youth has been framed from a deficit perspective.The author developed and validated a measure of positive youth development (pyd) for use with adolescents with ccs to address their unique circumstances.Expert ratings reduced 51 items selected from the connecting adolescents beliefs and behaviors survey to 16 items.Two exploratory factor analyses using orthogonal and oblique rotations further reduced the number of items to 12.Three factors emerged: pyd-connections to peers and schools (α = .81), pyd-connection to family (α = .82), and pyd-confidence (α = .89).Relations between these constructs and perceived health were assessed in healthy youth and youth with ccs.Youth with ccs reported feeling less healthy and worrying more about their health, and endorsed greater levels of feeling depressed.Compared with a matched sample of youth without ccs, youth with ccs had significantly higher correlations between both pyd-confidence and composite pyd, and degree of health worry and feeling depressed, as well as between connection to peers and school and feeling depressed.The findings highlight the need for a measure of pyd specifically for adolescents with ccs.(psyc info database record (c) 2019 apa, all rights reserved).

Journal: School psychology (Washington, D.C.) (2019)
Publication types: Journal Article
Effects of Work and Recovery Duration and Their Ratio on Cardiorespiratory and Metabolic Responses During Aerobic Interval Exercise.

Myrkos, a, smilios, i, zafeiridis, a, iliopoulos, s, kokkinou, em, douda, h, and tokmakidis, sp.Effects of work and recovery duration and their ratio on cardiorespiratory and metabolic responses during aerobic interval exercise.J strength cond res 36(8): 2169-2175, 2022-this study examined the effect of work and recovery durations and of work-to-rest ratio (wrr) on total exercise time and oxygen consumption (v̇ o2 max), on exercise time above 80, 90, and 95% of v̇ o2 max and hrmax, and on blood lactate concentrations during aerobic interval exercise.Twelve men (22.1 ± 1 year) executed, until exhaustion, 4 interval protocols at an intensity corresponding to 100% of maximal aerobic velocity.Two protocols were performed with work bout duration of 120 seconds and recovery durations of 120 (wrr: 1:1) or 60 seconds (wrr: 2:1), and 2 protocols with work bout duration of 60 seconds and recovery durations of 60 (wrr: 1:1) or 30 seconds (wrr: 2:1).When compared at equal exercise time, total v̇ o2 and exercise time at v̇ o2 above 80, 90, and 95% of v̇ o2 max were longer ( p 95% of v̇ o2 max and hrmax was higher ( p 90% of v̇ o2 max and hrmax.Blood lactate was lower ( p 95% of maximum) cardiorespiratory stimulus.Longer work bouts and higher work-to-recovery ratio seem to activate anaerobic glycolysis to a greater extent, as suggested by greater blood lactate concentrations.

Journal: Journal of strength and conditioning research (2022)
Publication types: Journal Article
Detection and monitoring of driver mutations by next-generation sequencing in squamous cell lung cancer patient and possible predictive biomarker of third generation EGFR-tyrosine kinase inhibitors.

Driver mutation detection and the development of targeted drugs have significantly improved survival of advanced lung adenocarcinoma patients with driver mutations.However, we still lack understanding of druggable mutations in patients with advanced squamous cell lung cancer (sqclc).Less than 10% of sqclc patients have egfr gene mutations, thus we have limited knowledge of biological molecular changes with first generation egfr-tyrosine kinase inhibitor (tki) resistance.We report a case of an sqclc patient treated with first-line platinum-doublet chemotherapy.After disease progression, the patient was administered first generation egfr-tki gefitinib based on next generation sequencing results.After five months, a second biopsy was performed and both the tumor and plasma samples indicated an acquired egfr exon 20 t790m mutation.The patient was subsequently administered azd9291, which resulted in disease control for a time.Our results indicate that a tp53 exon 8 mutation might act as a negative predictive biomarker for third generation egfr-tkis.

Journal: Thoracic cancer (2018)
Publication types: Case Reports;Journal Article