syedzayyan/paper_mill_benchmark huggingface dataset:
Depression, Anxiety, and Stress: How Should Clinicians Interpret the Total and Subscale Scores of the 21-Item Depression, Anxiety, and Stress Scales?

The 21-item depression, anxiety, and stress scales (dass-21) is a self-report measure that is easy to administer, quick to score, and is freely available.Widely used in diverse settings and populations, confirmatory factor analytic evidence has accumulated for a bifactor model underlying this multidimensional measure.Studies employing an exploratory bifactor approach to more closely examine its underlying structure and inter-relations of factors, however, have been scarce.This is unfortunate because confirmatory techniques often employ indirect ways of handling model misspecification, whereas exploratory methods enable more direct approaches.Moreover, more precise approaches to modeling an exploratory bifactor structure have not been examined with the dass-21.Based on several large samples of undergraduate students in the united states, the first two parts of the paper (studies 1 and 2) utilized both exploratory (m= 19.7 years of age) and confirmatory factor analytic methods (m= 19.7 years of age) following those presented by contemporary multidimensional modeling theorists.Building upon these results, the third part of the paper (study 3;m= 20.0 years of age) examined sensitivity-/specificity-related indices to provide cut-off score recommendations for a revised dass-21 instrument based on a newly identified and supported bifactor structure.Implications of these results are discussed in terms of taxonomy, challenges inherent in multidimensional modeling, and potential use of the revised dass-21 measure as a component of an actuarial decision-making strategy to inform clinical referrals.

Journal: Psychological reports (2019)
Publication types: Journal Article
Contribution of interleukin 27 serum level to pathogenesis and prognosis in children with immune thrombocytopenia.

Concepts surrounding the mechanisms of thrombocytopenia in itp have shifted from the traditional view of autoantibody mediated platelet destruction to more complex mechanisms in which impaired platelet production, t-cell-mediated effects, and disturbed cytokine profiles play a role.Interleukin 27 (il-27) plays pleiotropic roles in immunomodulation and autoimmune diseases.we aimed to determine the level of il-27 in patients with itp and its relationship to patient and disease characteristics as well as disease chronicity and response to treatment.sixty childrens with primary immune thrombocytopenia were consequetively enrolled in this study as well as 20 age and sex matched healthy controls.itp patients had significantly higher levels of il-27 than controls (770.6 and 373.8 pg/ml, respectively).Patients with acute itp had the highest levels of il-27 among patient groups, while patients in remission had the lowest il-27 levels (860.1and 622.9 pg/ml, respectively).Patients who received ivig and combined steroids plus ivig had significantly higher il-27 levels than others.Patients who received eltrombopag had significantly lower il-27 levels than others.il-27 seems to play a role in pathogenesis of childhood itp.Il-27 can be used as a predictor for disease occurrence as well as responsiveness to treatment.

Journal: Medicine (2022)
Publication types: Journal Article
(±)-hyperzewalsins A-D, four pairs of nor-monocyclic polyprenylated acylphloroglucinols with immunosuppressive activity from hypericum przewalskii maxim.

Three pairs of previously undescribed 2,3-nor-monocyclic polyprenylated acylphloroglucinols (mpaps), (±)-hyperzewalsins a-c, a pair of 1,2,3,4-nor-mpaps, (±)-hyperzewalsins d, along with one undescribed precursor, hyperzewalsin e, were isolated and identified from the aerial parts of hypericum przewalskii maxim.(hypericaceae), and their structures were confirmed by extensive spectroscopic analyses, and quantum-chemical calculations including electronic circular dichroism calculations and nmr calculations with a dp4+ analysis.Significantly, (±)-hyperzewalsins a-d represented the first nor-mpaps bearing carbon chain constitutions based on diverse highly degraded phloroglucinols.(±)-hyperzewalsins a-c were the rare nor-mpaps characterized by degradations of c-2 and c-3 in the core decorated by scissions of c-3/c-4 and c-1/c-2 bonds through retro-claisen reactions.(±)-hyperzewalsins d were the first examples of naturally occurring mpaps with the loss of c-1/2/3/4 in the phloroglucinol ring formed by cleavages of c-3/c-4 and c-1/c-6 bonds via retro-claisen and decarboxylation reactions.Plausible biogenetic pathways for the isolates were proposed.The isolates were evaluated for their immunosuppressive activity in lipopolysaccharide-stimulated murine splenocytes.

Journal: Phytochemistry (2021)
Publication types: Journal Article
CRISPR, Prime Editing, Optogenetics, and DREADDs: New Therapeutic Approaches Provided by Emerging Technologies in the Treatment of Spinal Cord Injury.

Spinal cord injury (sci) causes temporary disabilities or permanent effects including neuropathic pain and spastiscity.The damage often results from mechanical trauma, which in turn triggers the neuroinflammatory process.Neuroinflammation plays essential roles in the structural, biochemical, and cellular changes that take place in the spinal cord after the injury.Indeed, sci activates many different signaling pathways that coordinate the resulting cellular responses.While neuroinflammation serves as a physiological reaction to harmful stimuli, it is clear that long-lasting inflammatory response leads to aggravation of the neurodegenerative processes, becoming detrimental to recovery post-injury.In this context, we present some possible therapeutic targets in these activated signaling pathways and provide new perspectives for sci treatment based on recently developed technologies, including clustered regularly interspaced short palindromic repeats (crispr)-based methods (including prime editing), optogenetics, and designer receptor exclusively activated by designer drugs (dreadds).We critically analyze the recent advances in the deployment of these methods focusing on the control of the initial neuroinflammatory response.We then propose alternatives and provide new avenues for sci treatment based on these emerging technologies.

Journal: Molecular neurobiology (2020)
Publication types: Journal Article;Review
De Novo Mutations inEIF2B1Affecting eIF2 Signaling Cause Neonatal/Early-Onset Diabetes and Transient Hepatic Dysfunction.

Permanent neonatal diabetes mellitus (pndm) is caused by reduced β-cell number or impaired β-cell function.Understanding of the genetic basis of this disorder highlights fundamental β-cell mechanisms.We performed trio genome sequencing for 44 patients with pndm and their unaffected parents to identify causative de novo variants.Replication studies were performed in 188 patients diagnosed with diabetes before 2 years of age without a genetic diagnosis.eif2b1(encoding the e if2b complex α subunit) was the only gene with novel de novo variants (all missense) in at least three patients.Replication studies identified two further patients with de novo eif2b1variants.In addition to having diabetes, four of five patients had hepatitis-like episodes in childhood.The eif2b1de novo mutations were found to map to the same protein surface.We propose that these variants render the e if2b complex insensitive to e if2 phosphorylation, which occurs under stress conditions and triggers expression of stress response genes.Failure of e if2b to sense e if2 phosphorylation likely leads to unregulated unfolded protein response and cell death.Our results establish de novo eif2b1mutations as a novel cause of permanent diabetes and liver dysfunction.These findings confirm the importance of cell stress regulation for β-cells and highlight eif2b1's fundamental role within this pathway.

Journal: Diabetes (2020)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Interaction between Schwann cells and other cells during repair of peripheral nerve injury.

Peripheral nerve injury (pni) is common and, unlike damage to the central nervous system injured nerves can effectively regenerate depending on the location and severity of injury.Peripheral myelinating glia, schwann cells (scs), interact with various cells in and around the injury site and are important for debris elimination, repair, and nerve regeneration.Following pni, wallerian degeneration of the distal stump is rapidly initiated by degeneration of damaged axons followed by morphologic changes in scs and the recruitment of circulating macrophages.Interaction with fibroblasts from the injured nerve microenvironment also plays a role in nerve repair.The replication and migration of injury-induced dedifferentiated scs are also important in repairing the nerve.In particular, sc migration stimulates axonal regeneration and subsequent myelination of regenerated nerve fibers.This mobility increases sc interactions with other cells in the nerve and the exogenous environment, which influence sc behavior post-injury.Following pni, scs directly and indirectly interact with other scs, fibroblasts, and macrophages.In addition, the inter- and intracellular mechanisms that underlie morphological and functional changes in scs following pni still require further research to explain known phenomena and less understood cell-specific roles in the repair of the injured peripheral nerve.This review provides a basic assessment of sc function post-pni, as well as a more comprehensive evaluation of the literature concerning the sc interactions with macrophages and fibroblasts that can influence sc behavior and, ultimately, repair of the injured nerve.

Journal: Neural regeneration research (2021)
Publication types: Journal Article
Prediction of Disease Comorbidity Using HeteSim Scores based on Multiple Heterogeneous Networks.

Accumulating experimental studies have indicated that disease comorbidity causes additional pain to patients and leads to the failure of standard treatments compared to patients who have a single disease.Therefore, accurate prediction of potential comorbidity is essential to design more efficient treatment strategies.However, only a few disease comorbidities have been discovered in the clinic.Objective in this work, we propose pchs, an effective computational method for predicting disease comorbidity.Materials and methods we utilized the hete sim measure to calculate the relatedness score for different disease pairs in the global heterogeneous network, which integrates six networks based on biological information, including disease-disease associations, drug-drug interactions, protein-protein interactions and associations among them.We built the prediction model using the support vector machine (svm) based on the hete sim scores.Results and conclusion the results showed that pchs performed significantly better than previous state-of-the-art approaches and achieved an auc score of 0.90 in 10-fold cross-validation.Furthermore, some of our predictions have been verified in literatures, indicating the effectiveness of our method.

Journal: Current gene therapy (2019)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Immunohistochemical expression of VEGFR1 in non small cell lung carcinomas: Lower VEGFR1 expression is asociated with squamous cell carcinoma subtype and high max SUV values in18F-FDG PET.

To study the possible relation between immunohistochemical expression of vascular endothelial growth factor receptor 1 (vegfr1) and the maximum standardised uptake value (max suv) of18f-fdg pet in patients with non small cell lung cancer.Material and methods the study included 39 patients with nsclc (24 squamous cell carcinomas and 15 adenocarcinomas).According to the clinical stage, the patients were distributed as follows: 8 stage i, 7 stage ii, 15 stage iii and 9 stage iv.Immunohistochemical expression of vegfr1 was studied through the technique of tissue-matrix using tissue arrayer device (beecher instruments, sun prairie, wi), using the polyclonal antibody against vegfr1 (santa cruz biotechnology, california, usa).Results positive vegfr1 immunohistochemical expression was noted in 23 cases (59%).The number of positive tumours was not related with clinical stage but there was a different statistically significant association (p:.0009) between vegfr1 positivity and histological type, corresponding the greater percentages of positive results to adenocarcinomas (93.3%) versus in squamous cell carcinomas (37.5%).Likewise, max suv values were higher (p: .039) in negative vegfr1 carcinomas than in positive vegfr1 tumors (r: 4-32.1; 16.4+/-6.4 [median 16.1] vs.R: 3-47; 14.5+/-8.6 [12.8]).Conclusions our results led us to consider that in nsclc, the negative vegfr1 immunohistochemical expression is associated significantly with squamous cell carcinomas subtype and with higher max suv values in18f-fdg-pet.

Journal: Revista espanola de medicina nuclear e imagen molecular (2021)
Publication types: Journal Article
Visible-NIR Image Fusion Based on Top-Hat Transform.

The near-infrared band of the electromagnetic spectrum has become an important tool for enhancing image quality.Commonly, outdoor color images are degraded by bad weather conditions that lead to a loss of contrast and fine details in color images since light scattering produces attenuation and smoothing effects.Despite the fact that current visible-nir fusion methods achieve image enhancement features, some issues like edge preservation and color oversaturation still need to be addressed.In this work, a method that performs a selective visible-nir fusion of the most relevant image structures through top-hat transform is proposed.The performance of the method is evaluated by quantifying the new information added to the image and the change in color.Experimental results show a high degree of detail in preserving the edges while maintaining the color of the image.Moreover, the proposed method demonstrates that the image quality improvements were not significantly affected by a change in the color space.

Journal: IEEE transactions on image processing : a publication of the IEEE Signal Processing Society (2021)
Publication types: Journal Article
Single-cell RNA Sequencing Analysis Reveals New Immune Disorder Complexities in Hypersplenism.

Hypersplenism (hs) is a concomitant symptom of liver or blood disease.Not only does the treatment of hs face challenges, but the transcriptome of individual cells is also unknown.Here, the transcriptional profiles of 43,037 cells from four hs tissues and one control tissue were generated by the single-cell rna sequencing and nine major cell types, including t-cells, b-cells, nk cells, hematopoietic stem cells, neutrophil cells, mast cells, endothelial cells, erythrocytes, and dendritic cells were identified.Strikingly, the main features were the lack of ccl5+b-cells in hs and the presence of sesn1+b cells in hs with hepatocellular carcinoma (hs-hcc).In cell-cell interaction analysis, cd74-copa and cd94-hla-e in hs were found to be up-regulated.We further explored hs-specifically enriched genes (such as fkbp5,adar, and rps4y1) and found that fkbp5was highly expressed in hcc-hs, leading to immunosuppression.Taken together, this research provides new insights into the genetic characteristics of hsviacomprehensive single-cell transcriptome analysis.

Journal: Frontiers in immunology (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Design of an omnidirectional camouflage device with anisotropic confocal elliptic geometry in thermal-electric field.

The designed confocal elliptical core-shell structure can realize the omnidirectional camouflage effect without disturbing temperature and electric potential profiles as the directions of heat flux and electric current change.Based on the anisotropy of the confocal ellipse, the anisotropic effective parameters of the confocal elliptical core-shell structure are derived under different heat flux and electric current launching.Then, the matrix material should be anisotropic as the effective parameters to satisfy the omnidirectional camouflage effect, which is demonstrated numerically.In addition, we present a composite structure to realize the anisotropic matrix.The experimental results show that the camouflage device embedded in the composite structure can eliminate the scattering caused by the elliptical core under different directions of heat flux and electric current, thus achieving the omnidirectional thermal-electric camouflage effect experimentally.The omnidirectional camouflage effect in thermal and electric fields can greatly widen the application fields of this device with anisotropic geometry.

Journal: iScience (2022)
Publication types: Journal Article
A Flexible Plasmonic-Membrane-Enhanced Broadband Tin-Based Perovskite Photodetector.

Lead-free perovskite quantum dots (qds) have been widely investigated for optoelectronic devices because of their excellent electrical and optical properties.However, optoelectronic devices based on such lead-free perovskites still have much lower performance than those made of pb-based counterparts.Herein, we developed a lead-free photodetector with an enhanced broadband spectral response ranging from 300 to 630 nm.By balancing plasmonic near-field enhancement and surface energy quenching through precisely controlling the thickness of al2o3spacer between the cs sn br3qds and silver nanoparticle membrane, the photodetector with 5 nm thick al2o3experiences a maximum photocurrent enhancement of 6.5-fold at 410 nm, with a responsivity of 62.3 m a/w and detectivity of 4.27 × 1011jones.Moreover, its photocurrent shows a negligible decrease after 100 cycles of bending, which is ascribed to the tension-offset induced by the self-assembled nanoparticle membrane.The proposed plasmonic membrane enhancement provides a great potential for high-performance perovskite optoelectronic devices.

Journal: Nano letters (2021)
Publication types: Journal Article
Assessing oral comprehension with an eye tracking based innovative device in critically ill patients and healthy volunteers: a cohort study.

Communication of caregivers and relatives to patients is a major difficulty in intensive care units (icu).Patient's comprehension capabilities are variable over time and traditional comprehension tests cannot be implemented.Our purpose was to evaluate an oral comprehension test adapted for its automatic implementation using eye-tracking technology among icu patients.Methods prospective bi-centric cohort study was conducted on 60 healthy volunteers and 53 icu patients.Subjects underwent an oral comprehension test using an eye-tracking device: their results and characteristics were collected.The total duration of the test was 2 and a half minutes.Results while performing the test, 48 patients (92%) received invasive ventilation.Among healthy volunteers, the median rate of right answers was very high (93% [interquartile range 87, 100]), whereas it was lower (33% [20, 67]) for patients.For both groups, a significantly lower right answers rate was observed with advancing age (67% [27, 80] vs.27% [20, 38] among patients and 93% [93, 100] vs.87% [73, 93] among healthy volunteers, below and above 60 years of age, respectively) and in case of lack of a bachelor's degree (60% [38, 87] vs.27% [20, 57] among patients and 93% [93, 100] vs.87% [73, 93] among healthy volunteers).For patients, the higher the severity of disease was, the lower the rate of correct answers was.Conclusion the eye-tracking-adapted comprehension test is easy and fast to use among icu patients, and results seem coherent with various potential levels of comprehension as hypothesized in this study.

Journal: Critical care (London, England) (2022)
Publication types: Journal Article
Lycopene Inhibits IL-6 Expression by Upregulating NQO1 and HO-1 via Activation of Nrf2 in Ethanol/Lipopolysaccharide-Stimulated Pancreatic Acinar Cells.

In alcoholic pancreatitis, alcohol increases gut permeability, which increases the penetration of endotoxins, such as lipopolysaccharides (lps).Lps act as clinically significant triggers to increase pancreatic damage in alcoholic pancreatitis.Ethanol or lps treatment increases reactive oxygen species (ros) production in pancreatic acinar cells.Ros induce inflammatory cytokine production in pancreatic acinar cells, leading to pancreatic inflammation.The nuclear erythroid-2-related factor 2 (nrf2) pathway is activated as a cytoprotective response to oxidative stress, and induces the expression of nad(p)h quinone oxidoreductase 1 (nqo1) and heme oxygenase-1 (ho-1).Lycopene exerts anti-inflammatory and antioxidant effects in various cells.We previously showed that lycopene inhibits nadph oxidase to reduce ros and il-6 levels, and zymogene activation in ethanol or palmitoleic acid-treated pancreatic acinar cells.In this study, we examined whether lycopene inhibits il-6 expression by activating the nrf2/nqo1-ho-1 pathway, and reducing intracellular and mitochondrial ros levels, in ethanol and lps-treated pancreatic ar42j cells.Lycopene increased the phosphorylated and nuclear-translocated nrf2 levels by decreasing the amount of nrf2 sequestered in the cytoplasm via a complex formation with kelch-like ech1-associated protein 1 (keap1).Using exogenous inhibitors targeting nrf2 and ho-1, we showed that the upregulation of activated nrf2 and ho-1 results in lycopene-induced suppression of il-6 expression and ros production.The consumption of lycopene-rich foods may prevent the development of ethanol and lps-associated pancreatic inflammation by activating nrf2-mediated expression of nqo1 and ho-1, thereby decreasing ros-mediated il-6 expression in pancreatic acinar cells.

Journal: Antioxidants (Basel, Switzerland) (2022)
Publication types: Journal Article
Green synthesis and characterization of iron-oxide nanoparticles using Moringa oleifera: a potential protocol for use in low and middle income countries.

Green synthesized iron(iii) oxide (fe3o4) nanoparticles are gaining appeal in targeted drug delivery systems because of their low cost, fast processing and nontoxicity.However, there is no known research work undertaken in the production of green synthesized nano-particles from the ugandan grown moringa oleifera (mo).This study aims at exploring and developing an optimized protocol aimed at producing such nanoparticles from the ugandan grown moringa.Results while reducing ferric chloride solution with moringa oleifera leaves, iron oxide nanoparticles (fe3o4-nps) were synthesized through an economical and completely green biosynthetic method.The structural properties of these fe3o4-nps were investigated by ultra violet-visible (uv-vis) spectrophotometry, x-ray diffraction (xrd), energy dispersive x-ray spectroscopy (edx) and scanning electron microscopy (sem).These nanoparticles exhibited uv-visible absorption peaks at 225 nm (nm) for the sixth dilution and 228 nm for the fifth dilution which indicated that the nanoparticles were photosensitive and the sem study confirmed the spherical nature of these nanoparticles.The total synthesis time was approximately 5 h after drying the moringa leaves, and the average particle size was approximately 16 nm.Such synthesized nanoparticles can potentially be useful for drug delivery, especially in low and middle income countries (lmics).

Journal: BMC research notes (2022)
Publication types: Journal Article