syedzayyan/paper_mill_benchmark huggingface dataset:
Epidemiology of locomotive syndrome using updated clinical decision limits: 6-year follow-ups of the ROAD study.

Introduction locomotive syndrome (locomo) is defined by the japanese orthopaedic association (joa) as a condition requiring nursing care due to a decline in mobility resulting from musculoskeletal disorders.In 2020, the joa announced the new definition of locomo stage 3 and revision of clinical decision limits in stages of locomo.However, there are few reports on the epidemiological indices of locomo.This prospective cohort study aimed to investigate the prevalence, incidence, and association of poor prognosis with locomo stages.Materials and methods the third survey of the research on osteoarthritis/osteoporosis against disability (road) study was conducted during 2012-2013, examining a population-based cohort of 1575 participants (513 men and 1062 women, mean age 65.6 years).Three locomo risk tests were performed, and patients were classified into locomo stages 0, 1, 2, and 3.They were followed up for 6 years, and identical examination of locomo was performed in 3- and 6-year follow-ups.Data on patients' prognoses, including disability and death, were collected.Results the prevalence of locomo stages 1, 2, and 3 was 41.3, 14.9, and 11.6%, respectively.The incidence of locomo stages 1, 2, and 3 were 83.7, 23.0, and 18.6 per 1000 person-years, respectively.Compared with locomo stage 0, logistic regression analysis showed that locomo stage 3 significantly increased the risk of disability and mortality.In addition, each value of locomo risk tests for locomo stage 3 increased the risk of poor prognosis.Conclusion locomo stage 3 is a sensitive indicator of future disability and mortality.

Journal: Journal of bone and mineral metabolism (2022)
Publication types: Journal Article
Singlet fission relevant energetics from optimally tuned range-separated hybrids.

As a promising idea to design high-efficiency organic photovoltaics, singlet fission (sf) mechanism, i.e., generating two triplet excitons out of a single photon absorption, has recently come into the spotlight.Even though much effort has been devoted to this arena, accurately accounting for the sf process from the theoretical perspective has proven to be challenging.Herein, the sf energetics have thoroughly been investigated with the help of optimally tuned range-separated hybrid functionals (ot-rshs) in both gas and solvent phases.Taking a series of experimentally known sf chromophores as working models, we have proposed and validated several variants of ot-rsh approximations for the reliable prediction of the energy levels which match the crucial criteria for the sf process, namely, the negative singlet-triplet and triplet-triplet energy gaps.We scrutinize the role of the ot-rsh ingredients, i.e., the underlying density functional approximations, short- and long-range exact-like exchange, as well as the range-separation parameter, for our purpose.The newly designed ot-rshs outperform the standard rshs and other related schemes such as screened-exchange approximations as well as other density functionals from different rungs for describing the sf energetics.More importantly, it is unveiled that although the ot-rsh coupled with the polarizable continuum model, ot-rsh-pcm, as well as the screened versions, ot-srshs, which account for the screening effect by the electron correlation through the scalar dielectric constant have some advantages over gas-phase computations using ot-rshs, the energetics criteria of the sf process may not necessarily be satisfied.This in turn corroborates the idea of performing both the optimal tuning procedure and subsequent computations of the sf relevant energetics using ot-rshs as a more reliable and affordable framework, at least for the present purpose.The applicability of the proposed models is also put into broader perspective, where they are used for the computational design of several chromophores as promising candidates prone to utilization in the sf-based materials.Hopefully, our recommended ot-rshs can function as efficient models for both the theoretical modeling of sf chromophores and confirming the experimental observations in the field.

Journal: Physical chemistry chemical physics : PCCP (2020)
Publication types: Journal Article
Expression of CD44v6 and RCAS1 in Uterine Cervical Carcinoma Infected with Human Papillomavirus and Its Effect on Cell Proliferation and Differentiation.

To investigate the expression of cd44v6 and rcas1 and the presence of hpv in cervical cancer tissues, to determine serum rcas1 levels, and to evaluate these components in correlation with clinicopathologic features and survival.Methods a total of 52 patients consisting of 28 squamous cell carcinoma (scc) and 24 adenocarcinoma cases, were studied.Rcas1 and cd44v6 expression was evaluated using immunohistochemical staining.Hpv 16 and 18 e6 genes were detected using pcr, and serum rcas1 concentrations were measured using elisa.Associations between these factors and clinicopathologic features and survival were analyzed.Results cd44v6 expression was significantly higher in scc compared with that in adenocarcinoma (p<0.001).It also showed a significant relation to histologic grade (p<0.001) and tumor size (p=0.03).Rcas1 expression was higher in adenocarcinoma than in scc (p=0.001), and it showed a borderline relation with histological grade (p=0.057).Overall survival was not significantly different in both cd44v6 and rcas1 expression; however, figo stage (p=0.025) and tumor size (p=0.042) resulted statistically different.The pre-surgical treatment serum rcas1 levels were not associated with any clinicopathological variables.The presence of hpv 16 e6 was higher in scc, while the presence of hpv 18 e6 was higher in adenocarcinoma (p<0.001).Detection of hpv 16 e6 was significantly associated with expression of cd44v6.The presence of hpv both hpv 16 e6 and hpv 18 e6 was found in cancer tissues with rcas1 expression, but without any statistical significance.Conclusion cd44v6 and rcas1 expression seems to be involved in tumor proliferation and differentiation, but it is not implicated in the progression and invasion of cervical cancer infected by hpv.Pre-treatment levels of serum rcas1 in cervical cancer are not a diagnostic and predictive biomarker.

Journal: Asian Pacific journal of cancer prevention : APJCP (2022)
Publication types: Journal Article
Relationships among inferiority feelings, fear of negative evaluation, and social anxiety in Chinese junior high school students.

Introduction this study aimed to explore the relationship between feelings of inferiority and social anxiety in chinese junior high school students.In addition, it examined the potential mediating effect of fear of negative evaluation in this relationship.Methods a survey was administered to a sample of 734 chinese junior high school students.The feelings of inadequacy scale, brief fear of negative evaluation scale, and social avoidance distress scale were used.Results first, there were significant positive correlations between all subscales for the inferiority feelings, social anxiety, and fear of negative evaluation.Furthermore, fear of negative evaluation mediated the predictive effects of four inferiority subscales (i.e., self-esteem, academic ability, appearance, and physical ability) for social anxiety.However, the total score for the sense of inferiority and social confidence subscale lacked this mediating effect.Conclusion the inferiority feelings of self-esteem, academic ability, appearance, and physical ability may directly and indirectly predict social anxiety through fear of negative evaluation.

Journal: Frontiers in psychology (2022)
Publication types: Journal Article
aroF and cm2: potential molecular markers for the detection of stone-inhabiting Actinobacteria on cultural heritage sites.

Tangible archeological sites and stone monuments are naturally decayed and deteriorated over time, providing substances that can sustain life, although they provide a complicated ecosystem characterized by low nutrition and desiccation.Stone-inhabiting bacteria (sib) and especially members of the phylum actinobacteria dominate such environments, particularly the members of the family geodermatophilaceae.We used the published data of two confirmed sib species to mine their genomes for specific molecular markers to rapidly survey the presence of sib in cultural heritage material prior to further analysis.The search focused on the mycosporine-like amino acids (maas) synthesis pathway.Maas are intracellular compounds biosynthesized by the shikimic acid pathway to synthesize aromatic amino acids and were found related to abiotic resistance features in microorganisms.Based on genome mining, the dahp ii (aro f) and a homolog of the chorismate mutase gene (cm2) were found mostly in actinobacteria and few other species.After calibration on five stone-inhabiting actinobacteria (siab) species using conventional pcr, newly designed primers were successfully applied to environmental dna extracted from two egyptian pyramidal sites using a q pcr approach.This is the first report of aro f and cm2 as q pcr markers to detect siab from cultural heritage material prior to proceeding with further analysis (e.g., metagenomics and meta-barcoding analyses).

Journal: Archives of microbiology (2022)
Publication types: Journal Article
σ-Hole triel bonds in aluminium derivatives.

This communication reports the synthesis and x-ray characterization of a new mononuclear aluminium compound exhibiting an intramolecular σ-hole triel bonding interaction.It is compared with a dinuclear aluminium compound, previously reported by us, where the aluminium atoms participate in two concurrent σ-hole triel bonding interactions.To our knowledge, such behaviour has not been previously described in the literature.

Journal: Dalton transactions (Cambridge, England : 2003) (2023)
Publication types: Journal Article
Edible plant-derived exosomal microRNAs: Exploiting a cross-kingdom regulatory mechanism for targeting SARS-CoV-2.

The current covid-19 pandemic is caused by sars-co v-2 which belongs to coronaviridae family.Despite the global prevalence, there are currently no vaccines or drugs.Dietary plant derived exosome-like vesicles are known as edible nanoparticles (enps).Enps are filled with micro rnas (mi rnas), in bioavailable form.Recently, cross-kingdom regulation of human transcripts by plant mi rnas have been demonstrated.However, enp derived mi rnas targeting sars-co v-2 has not been described.Study design mature enp-derived mi rna sequences were retrieved from small rna sequencing datasets available in the literature.In silico target prediction was performed to identify mi rnas that could target sars-co v-2.Enps were isolated from ginger and grapefruit plants and the expression of sars-co v-2 targeting mi rnas were confirmed by q rt-pcr.Results from a total of 260 enp-derived mi rnas, we identified 22 mi rnas that could potentially target sars-co v-2 genome.11 mi rnas showed absolute target specificity towards sars-co v-2 but not sars-co v.Enps from soybean, ginger, hamimelon, grapefruit, tomato and pear possess multiple mi rnas targeting different regions within sars-co v-2.Interestingly, osa/cme mi r-530b-5p specifically targeted the ribosomal slippage site between orf1a and orf1b.We validated the relative expression of six mi rnas (mi r-5077, mi r-6300, mi r-156a, mi r-169, mi r-5059 and mi r-166 m) in ginger and grapefruit enps by rt-pcr which showed differential enrichment of specific mi rnas in ginger and grapefruit enps.Conclusion since administration of enps leads to their accumulation into lung tissues in vivo, enp derived mi rnas targeting sars-co v-2 genome has the potential to be developed as an alternative therapy.

Journal: Toxicology and applied pharmacology (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Parameter-Specific Morphing Reveals Contributions of Timbre to the Perception of Vocal Emotions in Cochlear Implant Users.

S research on cochlear implants (cis) has focused on speech comprehension, with little research on perception of vocal emotions.We compared emotion perception in ci users and normal-hearing (nh) individuals, using parameter-specific voice morphing.Design twenty-five ci users and 25 nh individuals (matched for age and gender) performed fearful-angry discriminations on bisyllabic pseudoword stimuli from morph continua across all acoustic parameters (full), or across selected parameters (f0, timbre, or time information), with other parameters set to a noninformative intermediate level.Results unsurprisingly, ci users as a group showed lower performance in vocal emotion perception overall.Importantly, while nh individuals used timbre and fundamental frequency (f0) information to equivalent degrees, ci users were far more efficient in using timbre (compared to f0) information for this task.Thus, under the conditions of this task, cis were inefficient in conveying emotion based on f0 alone.There was enormous variability between ci users, with low performers responding close to guessing level.Echoing previous research, we found that better vocal emotion perception was associated with better quality of life ratings.Conclusions some ci users can utilize timbre cues remarkably well when perceiving vocal emotions.

Journal: Ear and hearing (2022)
Publication types: Journal Article
RNA-seq-based transcriptome analysis of the anti-inflammatory effect of artesunate in the early treatment of the mouse cerebral malaria model.

Cerebral malaria (cm) is an important complication of malaria with a high mortality rate.Artesunate is recommended as the first-line artemisinin compound treatment for severe malaria.Due to the difficulty of obtaining brain tissue samples clinically, the use of animals to research host responses to cm parasite infections is necessary.Rodent malaria models allow for detailed time series studies of host responses in multiple organs.To date, studies on the transcriptome of severe malaria are only limited to the parasites in the peripheral blood of patients, and there is little data on the transcriptional changes in brain tissue in mice with cm treated with artesunate.Method and result in this study, fresh tissue samples (three biological replicates per mouse) from the same area of the brain in each animal were collected from the uninfected,plasmodium berghei anka-infected and artesunate-treated c57bl/6mice, and then transcriptome research was performed by the rna-seq technique.Differentially expressed genes (degs) included il-21,tnf,il-6,il-1β,il-10,ifng, and icam-1.Among which,il-6,il-10,tnf-αand il-1βwere further verified and validatedviaq rt-pcr and elisa.This revealed that il-1β(p< 0.0001),il-10(p< 0.05) and tnf-α(p< 0.05) were significantly up-regulated in the pb anka-infectedversusuninfected group, while il-1β(p< 0.0001) and tnf-α(p< 0.05) were significantly down-regulated after artesunate treatment.All degs were closely related to the top 3 artesunate treatment pathways, including the jak-stat signaling pathway, apoptosis, and toll-like receptor signaling pathway.Conclusion the mechanism of improving the prognosis of cerebral malaria by artesunate may not only involve the killing of plasmodium but also the inhibition of a cytokine storm in the host.This study provides new insights into the molecular mechanism by which artesunate improves the prognosis of cerebral malaria.

Journal: Molecular omics (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Primary lymphangioma of palatine tonsil.

Lymphangiomas are benign congenital tumors of the lymphatic system.Tonsillar lymphangiomas are extremely rare.Histopathological confirmation is essential to make the correct diagnosis.We report a case of primary lymphangioma of the left tonsil in a 17-year-old male patient who presented with difficulty in swallowing and was clinically diagnosed as a tonsillar cyst.

Journal: Journal of oral and maxillofacial pathology : JOMFP (2021)
Publication types: Case Reports;Journal Article
Atrial fibrillation detection using a automated electrocardiographic monitoring in a transient ischaemic attack service.

The vast majority of the transient ischaemic attacks (tia) services in uk reported significant delays in the initiation of the routine cardiac monitoring that may result in a significant number of missed atrial fibrillation (af) paroxysms and increased long-term risk of recurrent stroke.Automated continuous ecg monitoring (acem) system has shown promising results in terms of af detection but it is unclear if acem improves af detection in a rapid outpatient tia service.Objectives we assessed acem in patients with tia with the aim to significantly reduce the delay to initiate the cardiac monitoring and to enhance the yield of af detection in these patients.We also aimed to determine the impact of a more rapid initiation of acem on the 6-month risk of recurrent stroke/tia.Methods this is an observational, prospective before (phase 1: 1 july to 31 december 2018) versus after (phase 2: 1 january to 30 june 2019) study of the effect of acem, compared with routine initiation of 24h-holter ecg, in patients with tia assessed in our service.Results the phase 1 (n=136) and phase 2 (n=105) cohorts did not differ with regards to age, risk factors, duration of cardiac monitoring.The rate of newly detected af was significantly higher in phase 2 compared with phase 1 (9.52% vs 2.21%, p<0.001).The 6-month risk of recurrent stroke/tia was significantly lower in phase 2 compared with phase 1 (7.4% vs 1%, p=0.018).Conclusions early initiation of acem improves af detection after tia in a rapid tia service and is associated with a reduced risk of recurrent tia/stroke.

Journal: BMJ open quality (2022)
Publication types: Journal Article;Observational Study
Discovery of 6-[(3S,4S)-4-Amino-3-methyl-2-oxa-8-azaspiro[4.5]decan-8-yl]-3-(2,3-dichlorophenyl)-2-methyl-3,4-dihydropyrimidin-4-one (IACS-15414), a Potent and Orally Bioavailable SHP2 Inhibitor.

Src homology 2 (sh2) domain-containing phosphatase 2 (shp2) plays a role in receptor tyrosine kinase (rtk), neurofibromin-1 (nf-1), and kirsten rat sarcoma virus (kras) mutant-driven cancers, as well as in rtk-mediated resistance, making the identification of small-molecule therapeutics that interfere with its function of high interest.Our quest to identify potent, orally bioavailable, and safe shp2 inhibitors led to the discovery of a promising series of pyrazolopyrimidinones that displayed excellent potency but had a suboptimalin vivopharmacokinetic (pk) profile.Hypothesis-driven scaffold optimization led us to a series of pyrazolopyrazines with excellent pk properties across species but a narrow human ether-à-go-go-related gene (h erg) window.Subsequent optimization of properties led to the discovery of the pyrimidinone series, in which multiple members possessed excellent potency, optimalin vivo pk across species, and no off-target activities including no h erg liability up to 100 μm.Importantly, compound30(iacs-15414) potently suppressed the mitogen-activated protein kinase (mapk) pathway signaling and tumor growth in rtk-activated and krasmutxenograft modelsin vivo.

Journal: Journal of medicinal chemistry (2021)
Publication types: Journal Article
Psychological Counseling Service "Together" at University of Genoa: Students' Psychological Profile in Pre and Post Pandemic.

The present explorative study aims to analyze the profiles of students seeking help in the two areas (emotion/relation or learning areas) of a psychological counseling service for students at the university of genoa to better understand their request for support in pre- and post-pandemic periods.A total of 229 university students seeking for help from november 2018 to december 2021 completed a psychological battery investigating emotion regulation difficulties and pathological domains of personality (students taken in charge by the emotion/relation area) or motivation issues and anxiety and resilience levels (students taken in charge by the learning area).Regarding the emotion/relation area, results show that problems in emotion dysregulation, and especially in regulating positive emotions, are associated to several pathological domains of personality, such as psychoticism, antagonism, disinhibition, detachment, and negative affectivity.Among the learning area, motivational aspects concerning confidence in one's intelligence, academic self-efficacy, and learning goals are differently associated with anxiety and resilience above and beyond other aspects, such as attributions.Some limited but significant differences emerge from the comparison between pre- and post-covid periods: a reduction in detachment in students attending the emotion/relation area, an increase of students with high levels of anxiety in the learning area.These results support the importance of continually operating on emotional and motivational aspects to enhance the students' well-being and thus sustaining their academic careers.

Journal: Frontiers in psychology (2022)
Publication types: Journal Article
Biomechanical assessment of disease outcome in surgical interventions for medial meniscal posterior root tears: a finite element analysis.

The adverse consequences of medial meniscus posterior root tears have become increasingly familiar to surgeons, and treatment strategies have become increasingly abundant.In this paper, the finite element gait analysis method was used to explore the differences in the biomechanical characteristics of the knee joint under different conditions.Methods based on ct computed tomography and mr images, (i) an intact knee (ik) model with bone, cartilage, meniscus and main ligaments was established.Based on this model, the posterior root of the medial meniscus was resected, and (ii) the partial tear (pt) model, (iii) the entire radial tear (ert) model, and (iv) the entire oblique tear (eot) model were established according to the scope and degree of resection.Then, the (v) meniscus repair (mr) model and (vi) partial meniscectomy (pm) model were developed according to the operation method.The differences in stress, displacement and contact area among different models were evaluated under iso gait loading conditions.Results under gait loading, there was no significant difference in the maximum stress of the medial and lateral tibiofemoral joints among the six models.Compared with the medial tibiofemoral joint stress of the ik model, the stress of the pm model increased by 8.3%, while that of the mr model decreased by 18.9%; at the same time, the contact stress of the medial tibiofemoral joint of the ert and eot models increased by 17.9 and 25.3%, respectively.The displacement of the medial meniscus in the ert and eot models was significantly larger than that in the ik model (p < 0.05), and the tibial and femoral contact areas of these two models were lower than those of the ik model (p < 0.05).Conclusions the integrity of the posterior root of the medial meniscus plays an important role in maintaining normal tibial-femoral joint contact mechanics.Partial meniscectomy is not beneficial for improving the tibial-thigh contact situation.Meniscal repair has a positive effect on restoring the normal biomechanical properties of the medial meniscus.

Journal: BMC musculoskeletal disorders (2022)
Publication types: Journal Article