syedzayyan/paper_mill_benchmark huggingface dataset:
Circular RNA circ-CSPP1 regulates CCNE2 to facilitate hepatocellular carcinoma cell growth via sponging miR-577

Objective circ-centro-some/spindle pole-associated protein (cspp1) has been confirmed to be characterized in diverse human malignancies and its ectopic expression may regulate tumor progression and development.However, in hepatocellular carcinoma (hcc), its biological role, clinical significance and molecular mechanism are still unclear.Methods circ-cspp1 expression and its prognostic values in hcc tissues were detected by q rt-pcr or in situ hybridization (ish), and enriched by using rnase r.The functional experiments (circ-cspp1 was overexpressed or knocked down) were performed in hcc cells.The hcc cell growth was analyzed by cck-8 assay, transwell, wound healing and colony formation assays.The interation between circ-cspp1 and mi r-577/mi r-577 and cyclin e2 (ccne2) were determined by dual luciferase assay or rna binding protein immunoprecipitation (rip) assay.The rna fluorescence in situ hybridization (fish) assay was used to detect the subcellular distribution.Finally, an in vivo nude mouse tumor model was constructed.Results in hcc patients and cells, circ-cspp1 was aberrantly expressed, and its upregulation predicted poor prognosis, and closely correlated with tumor size and tnm stage.Circ-cspp1 resisted rnase r digestion, indicating it is a circular rna structure.Moreover, overexpression of circ-cspp1 promoted hcc cell viability, colony formation, migration, and invasion in vitro.Knockdown of circ-cspp1 showed contrary results.Circ-cspp1 acts as a mi r-577 sponge and positively regulated the target of mi r-577, ccne2.Besides, mi r-577 inhibitor rescued the suppressive effects of circ-cspp1 knockdown on hcc cell growth, whereas was completely reversed by silencing of ccne2.Finally, the in vivo experiments confirmed that circ-cspp1 knockdown regulated xenograft tumor volume and downregulated ccne2, p-rb, e2f1 and c-myc expression.Conclusion these findings revealed that circ-cspp1 contributed to hcc progression by positively regulating ccne2 via mi r-577, thus established its potential as new a prognostic and therapeutic marker for hcc patients.

Journal: Cancer Cell International (2020)
Publication types: Research Article;
Down-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice

Evidence has demonstrated that micro rna-342-5p (mi r-342-5p) is implicated in atherosclerosis (as), but little is known regarding its intrinsic regulatory mechanisms.Here, we aimed to explore the effect of mi r-342-5p targeting wnt3a on formation of vulnerable plaques and angiogenesis of as.Apo e −/− mice were fed with high-fat feed for 16 w to replicate the as vulnerable plaque model.Mi r-342-5p and wnt3a expression in aortic tissues of as were detected.The target relationship between mi r-342-5p and wnt3a was verified.Moreover, apo e −/− mice were injected with mi r-342-5p antagomir and overexpression-wnt3a vector to test their functions in serum lipid levels, inflammatory and oxidative stress-related cytokines, aortic plaque stability and angiogenesis in plaque of as mice.Mi r-342-5p expression was enhanced and wnt3a expression was degraded in aortic tissues of as mice and mi r-342-5p directly targeted wnt3a.Up-regulating wnt3a or down-regulating mi r-342-5p reduced blood lipid content, inflammatory and oxidative stress levels, the vulnerability of aortic tissue plaque and inhibited angiogenesis in aortic plaque of as mice.Functional studies show that depleting mi r-342-5p can stabilize aortic tissue plaque and reduce angiogenesis in plaque in as mice via restoring wnt3a.

Journal: Discover Nano (formerly known as Nanoscale Research Letters) (2021)
Publication types: Research Article;
The Path Deduction from Folk Art to Stage Art in the Change of Geographical Environment: Take Anhui Flower Drum Lantern as an Example

Anhui flower drum lantern, as a folk dance in the huaihe river basin, exudes the local cultural atmosphere and expresses the cultural color of song and dance drama incisively and vividly.At the same time, it is a sacrificial activity with social belief.As a typical example of the development of folk activities in anhui, it is an important intangible cultural heritage.With the accelerating construction of economic globalization and social innovation, the pursuit of “cultural confidence” has gradually become the belief of a country, a nation, and even everyone.More and more attention is being paid to the protection and dissemination of national intangible cultural heritage.The paper takes anhui flower drum lamp as the research object, combined with the characteristics of the regional nature, performance form, content, and cultural connotation of huagu lantern, by reading the social activities of the people on both sides of the huaihe river basin and using literature data, field visits, interviews, logical analysis, and other research methods.The origin, living environment, development historical context, cultural value, existing confusion, and development countermeasures of huaihe river are analyzed and discussed.This article helps to spread to the people the understanding of the evolution law of the traditional projects with the characteristics of generational attack.At the same time, it is also helpful to grasp the understanding of hua gu deng and the social life, social beliefs, social relations, and other factors on both sides of the huaihe river.This also make people further understand the social status and value function of the flower drum lantern along the huai river and it is of great practical significance to the dissemination of the same type of intangible cultural heritage.

Journal: Journal of Environmental and Public Health (2022)
Publication types: Research Article;
On the Feasibility of Integrating the Concept of Contemporary Green Environment Development in Creating Screen Prints by Digital Means

In the 21st century with the popularization of digital technology, as a printmaking born out of industrial printing, it has undergone a revolutionary evolution.Silkscreen printmaking is a type of print with high technology content, strong dependence on media, and no traditional load.Thirty years ago, as soon as silkscreen prints settled in china, they began artistic exploration and experimentation with a brand‐new attitude.Several generations of screen print workers have achieved it, expanded it, and made it independent in the fusion of modern language methods and oriental cultural spirit, and in the exploration of screen vocabulary, characteristics and character, it has an independent status and ranks among the international elite qualifications.This paper analyzes the root problems of high‐tech printmaking tools, materials, technical principles, and causes of aesthetics.Starting from the background of the concept of green development, it expounds its related concepts, analyzes the theoretical origin of green development, and clearly recognizes the high‐tech.The limitation of printmaking in the development space—that is, the expression language of digital plate making, is relatively correct in theory, so as to carry out step‐by‐step exploration and practice.

Journal: Journal of Environmental and Public Health (2022)
Publication types: Research Article;
On the Visual Construction of Environmental Factors in Modern Art Works from the Perspective of Painting Art

Since the twenty‐first century, with the continuous improvement of economic life, people have higher and higher requirements for life at present.Therefore, exploring better environmental art design has become a key concern of many people.This kind of environmental design can rely on the development of modern art in china.By analyzing the atmosphere of environmental factors and visual construction methods in modern art works, it can be integrated into the geographic positioning and map construction of slam in artificial intelligence.After analyzing the flavor of the times and visual construction of modern art, it is believed that slam algorithm can be combined with full convolution network fcn and superpixel crf and can better estimate the position in many dynamic scenes.Therefore, we should explore in different ways, integrate the art culture in modern art works with modern social science and technology, and realize the modernization of environmental art design.It can be seen that the slam system formed by combining with the visual construction of environmental factors in modern art works is more suitable for people’s life at the current stage.

Journal: Journal of Environmental and Public Health (2022)
Publication types: Research Article;
A natural food sweetener with anti-pancreatic cancer properties

Mogroside v is a triterpenoid isolated from the traditional chinese medical plant siraitia grosvenorii .Mogroside v has a high degree of sweetness and a low calorific content.Herein, we found that mogroside v possesses tumor growth inhibitory activity in in vitro and in vivo models of pancreatic cancer by promoting apoptosis and cell cycle arrest of pancreatic cancer cells (panc-1 cells), which may in part be mediated through regulating the stat3 signaling pathway.These results were confirmed in vivo in a mouse xenograft model of pancreatic cancer.In xenograft tumors, ki-67 and pcna, the most commonly used markers of tumor cell proliferation, were downregulated after intravenous administration of mogroside v.Terminal deoxynucleotidyl transferase d utp nick end labeling assays showed that mogroside v treatment promoted apoptosis of pancreatic cancer cells in the xenograft tumors.Furthermore, we found that mogroside v treatment significantly reduced the expression of cd31-labeled blood vessels and of the pro-angiogenic factor vascular endothelial growth factor in the xenografts, indicating that mogroside v might limit the growth of pancreatic tumors by inhibiting angiogenesis and reducing vascular density.These results therefore demonstrate that the natural, sweet-tasting compound mogroside v can inhibit proliferation and survival of pancreatic cancer cells via targeting multiple biological targets.

Journal: Oncogenesis (2016)
Publication types: Research Article;
MicroRNA-29 mediates anti-inflammatory effects and alleviation of allergic responses and symptoms in mice with allergic rhinitis

Background to investigate the role of micro rna-29 (mi r-29) in mice with allergic rhinitis (ar) and its underlying mechanism.Methods ar model was established in balb/c mice by intraperitoneal sensitization and intranasal challenge with ovalbumin (ova).Mi rna expression was examined in the nasal mucosa tissues of mice and patients with ar, and mi rna-29 was found to be downregulated.To unveil the role of mi rna-29 in ar, it was overexpressed in the nasal mucosa of ar mice by intranasal administration of mi rna-29 agomir.The symptoms of nasal rubbing and sneezing were recorded and evaluated.Mi r-29 expression, ova-specific immunoglobulin e (ig e) concentration, pro-inflammatory cytokines levels, eosinophils number, and cleaved caspase-3 and cd276 expression were examined in nasal mucosa tissues and nasal lavage fluid (nalf) by q rt-pcr, elisa, hematoxylin and eosin staining, western blotting, or immunohistochemistry, respectively.Tunel assay was used to analyze nasal mucosa cells apoptosis.Results decreased expression of mi r-29 was observed in ar, the symptoms of which were alleviated by overexpressing mi r-29.In addition, overexpression of mi r-29 markedly reduced the concentration of ova-specific ig e, the levels of il-4, il-6, il-10, and ifn-γ, the pathological alterations and eosinophils infiltration in the nasal mucosa.Furthermore, restoration of mi r-29 expression reduced nasal mucosa cell apoptosis.Moreover, overexpression of mi r-29 significantly attenuated cd276 m rna and protein levels in nasal mucosa cells.Conclusion mi r-29 mediated antiallergic effects in ova-induced ar mice by decreasing inflammatory response, probably through targeting cd276.Mi rna-29 may serve as a potential novel therapeutic target for the treatment of ar.

Journal: Allergy, Asthma & Clinical Immunology (2021)
Publication types: Research Article;
Chrysophanol suppresses hypoxia-induced epithelial-mesenchymal transition in colorectal cancer cells

Colorectal cancer (crc) is a common human malignancy, accounting for 600,000 death cases annually worldwide.Chrysophanol is a naturally occurring anthraquinone compound and exhibits anti‐neoplastic activities.This study aims to explore the biological effects of chrysophanol on crc metastasis and the relevant underlying mechanism.Cell proliferation assay, wound scratch assay, and transwell invasion assay were used to examine the effect of chrysophanol on proliferation, migration, and invasion of crc cells.Hypoxia‐inducible factor‐1α (hif‐1α) sh rna was utilized to transfect crc cells to examine the role of hif‐1α in chrysophanol suppression of hypoxia‐induced epithelial to mesenchymal transition (emt).The suppression effect of chrysophanol on hypoxia‐induced emt in vivo was also validated in xenograft tumor models.In the present study, our findings indicated that chrysophanol has the capability to suppress hypoxia‐induced emt in crc in vitro and in vivo , and the possible mechanism involved is the inhibition of hif‐1α via modulating pi3k/akt signaling pathway.Collectively, the results indicated that chrysophanol can be used as an emt and cancer metastasis inhibitor in the treatment of crc.Anat rec, 302:1561–1570, 2019.© 2019 american association for anatomy

Journal: The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology (2019)
Publication types: Research Article;
TRIM11 overexpression promotes proliferation, migration and invasion of lung cancer cells

Background tripartite motif containing 11 (trim11), a member of trim proteins, is overexpressed in high-grade gliomas and plays an oncogenic function in glioma biology.However, little is known about the role of trim11 in lung cancer.Methods we analyzed trim11 m rna expression in lung cancer tissues and adjacent non-neoplastic tissues by real-time pcr.We then explored the function of trim11 in lung cancer cells by small interfering rna-mediated downregulation of this protein followed by analyses of cell proliferation, migration and invasion.Results trim11 was highly expressed in lung cancer tissues and lung cancer cell lines.The higher expression of trim11 was correlated with the poorer prognosis of patients.Suppressing of trim11 expression in lung cancer cells with higher expression of trim11 (a549 and nci-h446 cells) significantly reduced cell growth, motility and invasiveness.We further demonstrated that knockdown of trim11 affected the expression of cell proliferation-related proteins (cyclin d1 and pcna), and epithelial-mesenchymal transformation-related proteins (vegf, mmp-2, mmp-9, twist1, snail and e-cadherin).The activity of erk and pi3k/akt was also suppressed in trim11 knocked down cells.Further experiments in lung cells with lower expression of trim11 (nci-h460 and nci-h1975 cells) with akt inhibitor suggested that trim11 may promote cell motility and invasiveness through akt pathway.Conclusions our results indicate that trim11 acts as an oncogene in lung cancer through promoting cell growth, migration and invasion.Our findings may have important implication for the detection and treatment of lung cancer.

Journal: Journal of Experimental & Clinical Cancer Research: (CR) (2016)
Publication types: Research Article;
Circ-TCF4.85 silencing inhibits cancer progression through microRNA-486-5p-targeted inhibition of ABCF2 in hepatocellular carcinoma

The current study aimed to explore the role of the circular rna circ‐tcf4.85 and its downstream target micro rna‐486‐5p (mi r‐486‐5p) in hepatocellular carcinoma (hcc) development.Circ‐tcf4.85 was detected to be highly expressed in hcc tissues.Next, we found that silencing of circ‐tcf4.85 repressed hcc cell proliferation, invasion, and migration, while enhancing apoptosis.In addition, biotin‐coupled probe pull‐down and mi rna capture assays, as well as fluorescence in situ hybridization, confirmed that circ‐tcf4.85 could bind to mi r‐486‐5p.In rescue experiments, mi r‐486‐5p had the potential to eliminate the tumor‐suppressive effects of circ‐tcf4.85 knockdown in hcc.Moreover, mi r‐486‐5p was shown to target abcf2 gene, which was positively regulated by circ‐tcf4.85.Finally, nude mice subcutaneously injected with si‐circ‐tcf4.85‐transfected hcc cells presented with inhibited xenograft tumor formation in vivo .Taken together, our results reveal that silencing of circ‐tcf4.85 suppresses hcc progression via mi r‐486‐5p‐targeted inhibition of abcf2 .

Journal: Molecular Oncology (2020)
Publication types: Research Article;
Decreased expression of long noncoding RNA XLOC_013703 promotes cell growth via NF-κB pathway in multiple myeloma

Long noncoding rnas (lnc rnas) are dysregulated in cancer and involved in oncogenic or tumor inhibitory processes.The aim of the study was to investigate the expression pattern of lnc rna xloc_013703 in multiple myeloma (mm) and to evaluate its biological role and potential significance.We found that xloc_013703 was significantly decreased in cd138 positive plasma cells and serum of mm patients compared to normal controls, and the decreased xloc_013703 expression was correlated with β2‐mg, serum‐free light chain (s‐flc) and revised international staging system.Rna‐fluorescence in situ hybridization results revealed that xloc_013703 was distributed both in the nucleus and in the cytoplasm of mm cells including h929, rpmi8226, and u266.Overexpression of xloc_013703 inhibited the proliferation of u266 cells and blocked the cell cycle in g1 stage, thus contributing to mm cell apoptosis.By contrast, knockdown of xloc_013703 promoted the growth of h929 cells.Western blot analysis confirmed that the expression of p‐iκbα and nuclear p65 was substantially increased in sh rna transfection groups compared to control groups, whereas overexpression of xloc_013703 reduced these expressions.In conclusion, we confirmed that the decreased expression of a novel lnc rna, xloc_013703, in mm.Xloc_013703 was involved in mm cell survival and proliferation via nuclear factor‐κb pathway which represents a potential therapeutic target for mm.© 2019 iubmb life, 71(9):1240–1251, 2019

Journal: IUBMB Life (2019)
Publication types: Research Article;
Reduced levels of miR-485-5p in HPV-infected cervical cancer promote cell proliferation and enhance invasion ability

Cervical cancer (cc) is the most common gynecological malignancy, with high incidence and mortality rates in china.The micro rna mi r‐485‐5p has previously been reported to serve as a negative regulator of tumorigenesis in breast cancer and hepatocellular carcinoma, and mi r‐485‐5p has been observed to be differentially expressed between cc and normal control tissue.Here, we confirmed that mi r‐485‐5p expression is lower in cc than in adjacent normal tissue and proceeded to investigate the effects of mi r‐485 on tumor behavior in cc cell lines.We report that mi r‐485‐5p transcription is decreased in hpv‐infected cc tissue, and levels of mi r‐485 in clinical samples are positively correlated with the 5‐year overall survival rate.The transwell assay showed that mi r‐485‐5p inhibited cell invasion and migration but had no influence on apoptosis and cell proliferation.Using a luciferase reporter assay, we demonstrated that mi r‐485‐5p partially abrogated cell migration and proliferation by targeting flot‐1 m rna.Transfection of hpv‐infected cervical carcinoma cells with an adenovirus vector encoding human flot‐1 partially diminished the inhibitory effects of mi r‐485 on cell invasion.Taken, together, these data demonstrated that mi r‐485‐5p suppresses the invasion of cancer cells by targeting flot‐1 in hpv‐infected cervical carcinoma cells.

Journal: FEBS Open Bio (2020)
Publication types: Research Article;
The F-box protein FBXO11 restrains hepatocellular carcinoma stemness via promotion of ubiquitin-mediated degradation of Snail

Expression of the f‐box protein fbxo11 has been shown to be down‐regulated in various tumors, but its role in hepatocellular carcinoma (hcc) progression remains unclear.Here, we examined the role of fbxo11 in hcc cell stemness.We report that fbxo11 expression is significantly decreased in hcc cells, and overexpression of fbxo11 decreased the expression of hcc stemness markers, aldh1 activity and sphere‐forming ability.In addition, overexpression of fbxo11 reduced the migration ability and epithelial‐mesenchymal transition of hcc cells.Mechanistically, overexpression of fbxo11 decreased the protein level, but not m rna level, of snail by directly interacting with snail and promoting snail degradation through the ubiquitin‐proteasome system.Overexpression of snail rescued the inhibitory effect of fbxo11 overexpression on hcc cell stemness.This study reveals the existence of a novel fbxo11/snail regulatory axis that is necessary for hcc cell stemness.

Journal: FEBS Open Bio (2020)
Publication types: Research Article;
LncRNA THOR increases osteosarcoma cell stemness and migration by enhancing SOX9 mRNA stability

Although the long non‐coding rna thor has been reported to promote cancer stem cell expansion in liver cancer and gastric cancer, its effects on osteosarcoma ( os ) cells remain unclear.Here, we investigated the roles of thor in the stemness and migration of os cells.We report that the level of thor is remarkably upregulated in os cell spheroids compared to that in os adherent cells.Thor overexpression increased spheroid formation ability and aldehyde dehydrogenase 1 ( aldh 1) activity in os adherent cells, and the opposite effect was observed in spheroids with thor knockdown.Additionally, the spheroids formed by os adherent cells exhibited a stronger migration ability, which was attenuated by thor knockdown, and thor overexpression increased os cell migration.Mechanistically, m rna stability, luciferase reporter, and rna – rna in vitro interaction assays indicated that thor can directly bind to the middle region of the sox9 3′‐untranslated region ( utr ), and enhances its m rna stability, thereby increasing its expression.Notably, sox 9 knockdown reduced the ability of thor overexpression to promote the stemness of os cells.These findings indicate that the lnc rna thor can promote the stemness and migration of os cells by directly binding to the middle region of sox9 3′ utr , thereby enhancing sox9 m rna stability and increasing its expression; thus, we provide information that may be of use in identifying potential targets for os treatment.

Journal: FEBS Open Bio (2019)
Publication types: Research Article;