syedzayyan/paper_mill_benchmark huggingface dataset:
Ovulation mitigates fatty liver associated with reproductive suppression and oxidative stress in Damaraland mole-rats (Fukomys damarensis).

Oxidative damage is often linked to reproduction; however, reproducing female damaraland mole-rats (fukomys damarensis) exhibit a reduction in oxidative damage relative to their non-reproductive, anovulatory, cohorts.Specifically, liver concentrations of malondialdehyde, a biomarker for lipid peroxidation, are significantly lower in reproducing females.We examined liver histology in reproductive, anovulatory and recently ovulating non-reproductive females, demonstrating an accumulation of lipid droplets only in the livers of anovulatory females and no fibrosis, cell death or inflammatory infiltrates in any group.Our observations suggest that anovulatory females experience a form of non-alcoholic fatty liver disease, which is reversed once they commence ovulation.We propose hormonal interactions that may underlie our observations.

Journal: Reproduction, fertility, and development (2020)
Publication types: Journal Article
Polycyclic aromatic hydrocarbons in coastal sediments of Southern Terengganu, South China Sea, Malaysia: source assessment using diagnostic ratios and multivariate statistic.

Surface sediments along the southern terengganu coast (≤7 km from the coast) were analyzed for polycyclic aromatic hydrocarbons (pahs).The concentrations of 16 usepa priority polycyclic aromatic hydrocarbons (σpah16) ranged from 2.59 to 155 ng g-1and their respective alkylated ranged between 8.80 and 24.90 ng g-1.Traces of acephenanthrylene, benzo[c]phenanthrene, thiophenic pah, and benzonaphthofuran were identified.Pah diagnostic ratios and cross-plots revealed that these sedimentary pah compounds are derived mainly from pyrogenic sources, primarily from biomass burning and petroleum combustion residues with minor petrogenic input.The high correlations between pyrogenic pahs to total pahs (r >0.73, p <0.5), and the bap/bep ratio to total pahs (r = 0.88, p <0.5), suggest that atmospheric deposition and urban runoff are the main deposition pathways.The concentrations of the pahs in the southern south china sea fall in the moderate contamination range of 100-1000 ng g-1.

Journal: Environmental science and pollution research international (2022)
Publication types: Journal Article
Stiff and Tough Hydrogels Prepared Through Integration of Ionic Cross-linking and Enzymatic Mineralization.

Enzymatic mineralization has become an effective approach to enhancing the stiffness of hydrogels for bone tissue engineering, but generally with limited toughness.On the other hand, double network cross-linking provides hydrogel with enhanced toughness.In this study, we integrated double cross-linking method with enzymatic mineralization to synthesize stiff and tough hydrogels.We have synthesized three kinds of sodium alginate-polyacrylamide (sa-pam) double-network hydrogels and systematically compared the composition and structure differences, mechanical properties, and biological properties of the different hydrogels in the absence and presence of mineralization.In particular, we examined the role of specific cross-linking ions, i.e., calcium, zinc and strontium ions, in modulating the mineralization process.Synergistic effect of ionic cross-linking and enzymatic mineralization was clearly observed with dramatic increase in compressive modulus.In particular, mineralized hydrogel cross-linked with sr2+showed the highest compressive young's modulus of 17.28 ± 3.56 mpa, which was 37 times of that of the original hydrogel.In addition, it had the highest tensile young's modulus at 2.60 ± 0.25 mpa and 84 ± 5.5% elongation at break.Such synergistic effect from sr2+was attributed to a more uniformed mineralization process due to the early initiation of a more homogeneous nucleation process and subsequent denser mineralized structure.Cellular study also suggested that cell proliferation, adhesion and osteogenic differentiation were improved as a result of enzymatic mineralization.Our results provided an effective way for the preparation of stiff and tough hydrogels with osteogenesis, and demonstrated potential in bone tissue engineering applications.Statement of significance: hydrogels with excellent stiffness, stability and biocompatibility have attracted significant attentions in the bone tissue engineering applications.Our results suggested that the synergistic effect of ionic cross-linking and enzymatic mineralization rendered more enhancement of the compressive and tensile stiffness of sa-pam dn hydrogels, as well as the toughness, swelling stability and cellular response.In particular, mineralized hydrogel cross-linked with sr2+showed the highest compressive young's modulus of 17.28 ± 3.56mpa, which was 37 times of that of the original hydrogel.Such synergistic effect from sr2+was attributed to a more uniformed mineralization process.The cell proliferation, adhesion and osteogenic differentiation were greatly improved as a result of enzymatic mineralization, where the mscs cultured on strontium ion cross-linked mineralized hydrogel showed the best performance.

Journal: Acta biomaterialia (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
ANXC7 Is a Mitochondrion-Localized Annexin Involved in Controlling Conidium Development and Oxidative Resistance in the Thermophilic FungusThermomyces lanuginosus.

Annexins (anxs) are widely expressed and structurally related proteins which play multiple biological roles in animals, plants, and fungi.Although anxs have been localized to the cytosol and the cell membrane and the molecular basis of the four annexin repeats is well established, thein vivoroles of these proteins are still far from clear, particularly with regard to the filamentous fungi.thermomyces lanuginosus, a thermophilic fungus, is widely used in the fermentation industry; however, the role of anxin this organism is unknown.In this study, a single anxhomologue (anxc7) was identified and characterized in t.Lanuginosus.The expression pattern indicated that anxc7is closely associated to conidium development, and it accumulated in the mitochondria of the forming conidia.The deletion of anxc7(δanxc7) resulted in no obvious phenotype related to colony growth on solid cm medium.However, whenδanxc7was grown in cm liquid culture, the mycelium masses appeared to be larger and looser compared to the wild-type.Additionally, the dry weight of the mutant mycelia was significantly increased.Under conditions that compromise cell-wall integrity,δanxc7was less vulnerable than the wild-type with regard to such damage.Moreover, based on a surface hydrophobicity test, theδanxc7strain was clearly less hydrophobic.The growth ofδanxc7was inhibited when grown under selected stress conditions, particularly with regard to salt stress; however, the oxidative resistance to exogenous h2o2inδanxc7was increased, and endogenous h2o2levels within theδanxc7were lower than in the wild-type, thereby suggesting that the anxc7specifically controls oxidative resistance.Based on microscopic observation, 4-day-conidia were more prevalent than 5-day conidia on the conidiophore stalk ofδanxc7, even though theδanxc7demonstrated an increased production of conidia during these days, indicating precocious conidial maturation and shedding from the conidiophore stalk in this strain.Taken together, our data indicate that anxc7localizes to the mitochondria and is involved in controlling conidium development and oxidative resistance in t.Lanuginosus.

Journal: Frontiers in microbiology (2018)
Publication types: Journal Article
Arrhythmogenic Right Ventricular Cardiomyopathy in an Older Private Pilot.

Arrhythmogenic right ventricular cardiomyopathy (arvc) results in progressive replacement of right ventricular myocardium with fibrofatty tissue.This condition commonly presents with sudden cardiac death, cardiac arrest, or sustained ventricular tachycardia among young adults.Considering the likelihood of sudden incapacitation, a diagnosis of arvc usually signals the end of a career in aviation.However, the morbidity pattern among those diagnosed later in life may differ as compared to the young.case report:a private pilot, in his 60s, applied for renewal of an icao class 2 medical certificate a decade after his previous medical certificate had expired.He declared history of hospitalization due to a heart-related condition.Further information revealed that he had been diagnosed as a case of arvc and that he had declined to be implanted with an implantable cardioverter-defibrillator.Recent clinical examination and related cardiovascular investigations were normal except echocardiogram findings of left ventricular hypertrophy and left atrial enlargement.The treating cardiologist, in the absence of history of syncope and evidence of right ventricular dysfunction or sustained ventricular tachycardia, considered this condition to be stable with minimal risk of malignant arrhythmia.discussion:this case report discusses aeromedical decision making in a pilot found to have arvc late in life.Despite the progressive nature of the condition with its attendant risk of incapacitation, considering the age at diagnosis and absence of any high-risk features of arvc, the regulator decided to issue the medical certificate with safety pilot restriction to enable this pilot to return to aviation activities.sharma s.arrhythmogenic right ventricular cardiomyopathy in an older private pilot.Aerosp med hum perform.2022; 93(2):111-115.

Journal: Aerospace medicine and human performance (2022)
Publication types: Case Reports;Journal Article
Microbial burden on environmental surfaces in patient rooms before daily cleaning-Analysis of multiple confounding variables.

S estimated levels of microbial burden on hospital environmental surfaces vary substantially among published studies.Cultures obtained during a cluster-controlled crossover trial of a quaternary ammonium (quat) disinfectant versus an improved hydrogen peroxide (ihp) disinfectant provided additional data on the amount of microbial burden on selected surfaces.Methods rodac plates containing d/e neutralizing agar were used to sample a convenience sample of 5-8 high-touch surfaces in patient rooms on 2 medical wards, an intensive care unit, and a step-down unit at a large hospital.Before routine daily cleaning, samples were obtained in varying rooms over an 11-month period.Rodac plates (1 per surface sampled) were incubated for 72 hours, and aerobic colony counts per plate (accs) were determined.Statistical analysis was used to determine the potential impact on accs of study period, cleaning compliance rate, disinfectant used, ward, surface sampled, and isolation room status.Results overall, 590 cultures were obtained on quat wards and 589 on ihp wards.Multivariable regression analysis revealed that mean accs differed significantly by site (p< .001), type of ward (p< .001), isolation room status (p= .039), and study period (p= .036).The highest mean accs per rodac plate were on toilet seats (112.8), bedside rails (92.0), and bathroom grab bars (79.5).Conclusions the combination of factors analyzed revealed that estimating microbial burden is complex and is affected by multiple factors.Additional studies should evaluate individual sites, ward types, cleaning and disinfection practices, and isolation room status.

Journal: Infection control and hospital epidemiology (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Why is COVID-19 associated with mental illness?

The covid-19 pandemic has taken a toll on people's mental health.Besides the indirect impact of the pandemic, a diagnosis of covid-19 is itself associated with a greater risk of subsequent mental illness.Conversely, people with an existing psychiatric diagnosis are at an increased risk of getting covid-19.Here, we discuss why this is the case.

Journal: Med (New York, N.Y.) (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Vitex negundoL. derived specialized molecules unveil the multi-targeted therapeutic avenues against COPD: a systems pharmacology approach.

Introduction chronic obstructive pulmonary disease (copd) is an inflammatory disease caused by increasing breathing passage obstruction which completely disrupts human homeostasis.Some patients require lung transplantation or long-term oxygen therapy.Copd is one of the noxious diseases and its fourth leading cause of death around the globe.There is an immediate need for potential drug development to tackle this serious disease.Folk medicines are used to combat complex diseases that have shown effectiveness in the treatment of breathing diseases.vitex negundo l.Is an ethnobotanically important medicinal plant used for various ailments and modulates human cellular events.This shrub has diverse specialized metabolites and is being used as complementary medicine in various countries.Though systems-level understanding is there on the mode of action, the multi-target treatment strategy for copd is still a bottleneck.Methods in this investigation, systems pharmacology, cheminformatics, and molecular docking analyses were performed to unravel the multi-targeted mechanisms of v.Negundo l.Potential bioactives to combat copd.Results cheminformatics analysis combined with the target mining process identified 86 specialized metabolites and their corresponding 1300 direct human receptors, which were further imputed and validated systematically.Furthermore, molecular docking approaches were employed to evaluate the potential activity of identified potential compounds.In addition, pharmacological features of these bioactives were compared with available copd drugs to recognize potential compounds that were found to be more efficacious with higher bioactive scores.Conclusions the present study unravels the druggable targets and identifies the bioactive compounds present in v.Negundo l., that may be utilized for potential treatment against copd.However, furtherin vivoanalyses and clinical trials of these molecules are essential to deciphering their efficacy.

Journal: Frontiers in bioscience (Landmark edition) (2022)
Publication types: Journal Article
Chondroprotective Effects Of Intra-Articular Hyaluronic Acid And Triamcinolone In Murine Model Of Osteoarthritis.

Osteoarthritis is a heterogeneous disease of joints that affects mainly older population.Definitive cure of osteoarthritis is still undiscovered.This study was designed to evaluate and compare the chondroprotective effects of hyaluronic acid and triamcinolone in murine model of osteoarthritis.Methods this laboratory based experimental study was carried out in pharmacology department, army medical college rawalpindi, from april-june 2019.Osteoarthritis was induced by medial menisectomy and anterior cruciate ligament resectioning of knee joints of twenty-four rats which were then divided into three groups with eight rats in each.Group i, ii and iii were named control, hyaluronic acid and triamcinolone groups respectively, which were given intra-articular injections of these drugs once weekly for four consecutive weeks and then gait pattern was scored.Animals were sacrificed thereafter and samples were collected for histopathological analysis.Results comparison of gait score of control, hyaluronic acid and triamcinolone groups exhibited a p value of <0.01 while intergroup comparison between group i and ii, group i and iii and group ii and iii depicted p-value of <0.001, 0.016 and 0.003 respectively.Likewise, collective histopathological analysis of the three groups showed p-value of <0.01 while intergroup comparison of group i and ii, group i and iii and group ii and iii showed p-value of <0.001 for all.Conclusions comparison of control group with treatment groups proved chondroprotective effects of hyaluronic acid and triamcinolone.Additionally, hyaluronic acid proved to provide better chondroprotection as compared to triamcinolone.

Journal: Journal of Ayub Medical College, Abbottabad : JAMC (2022)
Publication types: Journal Article
Improved extraction efficiency of CsPbBr3perovskite light-emitting diodes due to anodic aluminum oxide nanopore structure.

In this work, we investigate the improvement in the performance of a cs pb br3perovskite light-emitting diode (pe led) due to an anodic aluminum oxide (aao) nanopore structure.The aao structure in the cs pb br3pe led structure can improve the light extraction efficiency of cs pb br3pe leds in two ways: the emission light in the side direction being redirected to the normal direction due to the light scattering effect caused by aluminum oxide nanopores and the effective emission area as a result of the rough surface of the aao structure.The peak luminance, current efficiency, and external quantum efficiency (eqe) were 11,460 cd/m2, 2.03 cd/a, and 0.69% at a bias of 6.0 v, respectively.For comparison, the luminance, current efficiency, and eqe values of cs pb br3pe leds with the aao structure using 50 v of pore-expanding voltage demonstrated improvements of 282%, 190%, and 1280%, respectively, over cs pb br3pe leds without the aao structure.

Journal: Scientific reports (2022)
Publication types: Journal Article
Reliable quantification of Cytomegalovirus DNAemia in Letermovir treated patients.

Polymerase chain reaction (pcr) based methods are a fast and sensitive approach to detect and monitor viral load in cytomegalovirus (cmv) patients.Letermovir (lmv) acts at a late stage during the cmv replication cycle and does not inhibit cmv dna replication per se.Therefore, quantitative nucleic acid amplification testing might lead to the overestimation of viral load in patients treated with lmv and underestimate treatment success.To study this discrepancy, we treated infected cells with lmv or ganciclovir (gcv) and compared viral progeny dna levels.Prior to nucleic acid extraction and q pcr measurements we pretreated cell lysates and cell culture supernatants from infected cells with dnase i.This step assumes the degradation of dna which is not protected from a viral capsid.Lmv treatment did not reduce genomic copies (gc) in samples from whole cell lysates compared to samples treated with gcv.Dnase treatment prior to dna extraction, decreased gc in the lmv treated group to comparable levels as seen in the gcv group.In cell culture supernatants, lmv or gcv treatment led to an equivalent reduction of cmv gc.In this case, dnase treatment exerted a negligible effect on both groups.We conclude that the accumulation of concatemeric dna within cells seems to be a confounding variable when monitoring lmv efficacy via q pcr.However, q pcr shows to be a reliable method to evaluate antiviral efficacy of lmv in cell free specimens.These results have strong clinical implications for the monitoring of cmv therapy during lmv treatment.

Journal: Antiviral research (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Daidzein supplementation improved fecundity in sows via modulation of ovarian oxidative stress and inflammation.

Adequate ovarian hormones secretion is essential for pregnancy success.Oxidative damage and following inflammation can destroy the ovarian normal function in mammals.Daidzein (dai) is a classical isoflavonic phytoestrogen with specific oestrogenic activity.This study aimed to explore the effects of daidzein supplementation on fertility and ovarian characteristics of sows through biochemical analysis and rna-seq technology.Twelve multiparous yorkshire × landrace sows were randomly divided into con and dai groups.We found that dai increased total number of embryos as well as p4 and e2 levels of serum.Dai not only elevated the activities of t-aoc and gsh-px, but also tended to decrease the content of mda and il-6 in the serum.In ovary, rna-seq identified 237 differentially expressed genes (degs), and go analysis showed that these degs were linked to functions associated with immune dysfunction.Moreover, string analysis demonstrated that most interacting nodes were tlr-4, lcp2, and cd86.Furthermore, dai decreased the content of mda, il-1β, il-6, and tnf-α, and increased the activities of t-aoc and cat in ovarian tissue.Interestingly, a partial mantel correlation showed that t-aoc was the strongest correlation between the ovarian dataset and selected degs.Additionally, dai supplementation not only increased the protein expressions of nrf2, ho-1, and nqo1, but also decreased the protein expressions of tlr-4, p-nfκb, p-akt, and p-iκbα.Altogether, our results indicated that dai could ameliorate ovarian oxidative stress and inflammation in sows, which might be mediated by suppressing the tlr4/nf-κb signaling pathway and activating the nrf2/ho-1 signaling pathway.

Journal: The Journal of nutritional biochemistry (2022)
Publication types: Journal Article;Randomized Controlled Trial, Veterinary;Research Support, Non-U.S. Gov't
Nearly maximal violation of the Mermin-Klyshko inequality with multimode entangled coherent states.

Entangled coherent states for multiple bosonic modes, also referred to as multimode cat states, not only are of fundamental interest but also have practical applications.The nonclassical correlation among these modes is well characterized by the violation of the mermin-klyshko inequality.We here study mermin-klyshko inequality violations for such multi-mode entangled states with rotated quantum-number parity operators.It is shown that the mermin-klyshko signal obtained with these operators can approach the maximal value even when the average quantum number in each mode is only 1, and the inequality violation exponentially increases with the number of entangled modes.This is in distinct contrast with the framework based on displaced parity operators, with which a nearly maximal mermin-klyshko inequality violation requires the size of the cat state to be increased by about 15 times.

Journal: Optics express (2019)
Publication types: Journal Article
Semaphorin 1a-mediated dendritic wiring of theDrosophilamushroom body extrinsic neurons.

Significance the adult drosophilamushroom body (mb) is one of the most extensively studied neural circuits.However, how its circuit organization is established during development is unclear.In this study, we provide an initial characterization of the assembly process of the extrinsic neurons (dopaminergic neurons and mb output neurons) that target the vertical mb lobes.We probe the cellular mechanisms guiding the neurite targeting of these extrinsic neurons and demonstrate that semaphorin 1a is required in several mb output neurons for their dendritic innervations to three specific mb lobe zones.Our study reveals several intriguing molecular and cellular principles governing assembly of the mb circuit.

Journal: Proceedings of the National Academy of Sciences of the United States of America (2022)
Publication types: Journal Article
Selective conservative treatment for anterior abdominal gunshot: a literature narrative review.

The nonoperative treatment of anterior abdominal gunshot wounds remains controversial.This article presents a narrative review of the literature after the selection of studies in electronic databases (pub med, cochrane library and lilacs), with the intention of evaluating the clinical and diagnostic tools that should be part of conservative selective approach of these lesions.It was observed that a nonoperative selective treatment can be effectively and safely used, when performed by a trained interdisciplinary team, working in adequate trauma centers.The selective nonoperative treatment is associated with a decrease in negative and nontherapeutic laparotomies, reducing the incidence of complications.It also contributes to the reduction of hospital costs.

Journal: Revista do Colegio Brasileiro de Cirurgioes (2020)
Publication types: Journal Article;Review