syedzayyan/paper_mill_benchmark huggingface dataset:
Stauntonia hexaphyllaExtract Ameliorates Androgenic Alopecia by Inhibiting Androgen Signaling in Testosterone-induced Alopecia Mice.

Stauntonia hexaphyllahas been a traditional folk remedy for alleviating fever and providing anti-inflammatory properties.Androgenetic alopecia (aga) is the most common form mediated by the presence of the dihydrotestosterone (dht).Objectives in this study, we evaluated the effects of an extract of s.Hexaphyllaon aga models and its mechanisms of action.Methods we studied s.Hexaphyllaextract to evaluate 5α-reductase and androgen receptor (ar) levels, apoptosis, and cell proliferation in vitro and in vivo.In addition, paracrine factors for androgenic alopecia, such as transforming growth factor beta-1 (tgf-β1) and dickkopf-a (dkk-1), were examined.Apoptosis was investigated, and the evaluation of proliferation was examined with cytokeratin 14 (ck-14) and proliferating cell nuclear antigen (pcna).Results in human follicular dermal papilla cells, the 5α-reductase and ar were decreased following s.Hexaphyllatreatment, which reduced the bax/bcl-2 ratio.Histologically, the dermal thickness and follicle number were higher in the s.Hexaphyllagroups compared with the aga group.In addition, the dht concentration, 5α-reductase, and ar were decreased, thereby downregulating tgf-β1 and dkk-1 expression and upregulating cyclin d in s.Hexaphyllagroups.The numbers of keratinocyte-positive and pcna-positive cells were increased compared to those in the aga group.Conclusions the present study demonstrated that the s.Hexaphyllaextract ameliorated aga by inhibiting 5α-reductase and androgen signaling, reducing aga paracrine factors that induce keratinocyte (kc) proliferation, and inhibition apoptosis and catagen prematuration.

Journal: Iranian journal of pharmaceutical research : IJPR (2022)
Publication types: Journal Article
Transplanted embryonic retinal stem cells have the potential to repair the injured retina in mice.

Stem cell transplantation has been reported as one of the promising strategies to treat retinal degenerative diseases.But, the application and the role of retina stem cells (rscs) in the treatment of patients with retinal degenerative diseases have not been fully revealed.This study aimed to investigate the potential role of transplantation of the embryo-derived rscs into the vitreous cavity in repairing the damaged retina in mice.Methods rscs were isolated from kunming mice e17 embryonic retina and ciliary body tissues, and labeled with 5-bromo-2'-deoxyuridin (brd u).Retinal optic nerve crush injury was induced in left eyes in male kunming mice by ring clamping the optic nerve.The 6th -generation of brd u-labeled rscs were transplanted into the damaged retina by the intravitreal injection, and saline injected eyes were used as the control.Hematoxylin and eosin histological staining, and brd u, nestin and pax6 immunostaining were performed.Electroretinogram (erg) was used for assessing the electrical activity of the retina.Results embryo-derived rscs were identified by the positive stains of pax6 and nestin.Brd u incorporation was detected in the majority of rscs.The damaged retina showed cellular nuclear disintegration and fragmentation in the retinal tissue which progressed over the periods of clamping time, and decreased amplitudes of a and b waves in erg.In the damaged retina with rscs transplantation, the positive staining for brd u, pax6 and nestin were revealed on the retinal surface.Notably, rscs migrated into the retinal ganglion cell layer and inner nuclear.Transplanted rscs significantly elevated the amplitudes of a waves in retina injured eyes.Conclusions embryonic rscs have similar characteristics to neural stem cells.Transplantation of rscs by intravitreal injection would be able to repair the damaged retina.

Journal: BMC ophthalmology (2021)
Publication types: Journal Article
Contralateral Effects of Unilateral Strength and Skill Training: Modified Delphi Consensus to Establish Key Aspects of Cross-Education.

Cross-education refers to increased motor output (i.e., force generation, skill) of the opposite, untrained limb following a period of unilateral exercise training.Despite extensive research, several aspects of the transfer phenomenon remain controversial.Methods a modified two-round delphi online survey was conducted among international experts to reach consensus on terminology, methodology, mechanisms of action, and translational potential of cross-education, and to provide a framework for future research.Results through purposive sampling of the literature, we identified 56 noted experts in the field, of whom 32 completed the survey, and reached consensus (75% threshold) on 17 out of 27 items.Conclusion our consensus-based recommendations for future studies are that (1) the term 'cross-education' should be adopted to refer to the transfer phenomenon, also specifying if transfer of strength or skill is meant; (2) functional magnetic resonance imaging, short-interval intracortical inhibition and interhemispheric inhibition appear to be promising tools to study the mechanisms of transfer; (3) strategies which maximize cross-education, such as high-intensity training, eccentric contractions, and mirror illusion, seem worth being included in the intervention plan; (4) study protocols should be designed to include at least 13-18 sessions or 4-6 weeks to produce functionally meaningful transfer of strength, and (5) cross-education could be considered as an adjuvant treatment particularly for unilateral orthopedic conditions and sports injuries.Additionally, a clear gap in views emerged between the research field and the purely clinical field.The present consensus statement clarifies relevant aspects of cross-education including neurophysiological, neuroanatomical, and methodological characteristics of the transfer phenomenon, and provides guidance on how to improve the quality and usability of future cross-education studies.

Journal: Sports medicine (Auckland, N.Z.) (2021)
Publication types: Journal Article
Diagnostic Performance of F-18 Fluorocholine PET/CT for Parathyroid Localization in Hyperparathyroidism: a Systematic Review and Meta-Analysis.

The purpose of the current study was to investigate the diagnostic performance of f-18 fluorocholine (fch) positron emission tomography/computed tomography (pet/ct) for localization of hyperfunctioning parathyroid gland in patients with hyperparathyroidism (hpt) through a systematic review and meta-analysis.The medline/pub med and embase database, from the earliest available date of indexing through april 30, 2018, were searched for studies evaluating the diagnostic performance of f-18 fch pet/ct for localization of hyperfunctioning parathyroid gland in patients with hpt.We determined the sensitivities and specificities across studies, calculated positive and negative likelihood ratios (lr+ and lr-), and constructed summary receiver operating characteristic curves.Across eight studies (272 patients), the pooled sensitivity for f-18 fch pet/ct was 0.90 (95% ci, 0.86-0.94) without heterogeneity (i2= 7.1) and a pooled specificity of 0.94 (95% ci, 0.90-0.96) with heterogeneity (i2= 79.8).Likelihood ratio (lr) syntheses gave an overall positive likelihood ratio (lr+) of 5.3 (95% ci, 1.2-24.3) and negative likelihood ratio (lr-) of 0.15 (95% ci, 0.08-0.29).The pooled diagnostic odds ratio (dor) was 38 (95% ci, 8-174).Hierarchical summary receiver operating characteristic (roc) curve indicates that the areas under the curve were 0.9492 (se, 0.0215).In meta-regression analysis, no definite variable was the source of the study heterogeneity.The current meta-analysis showed the high sensitivity and specificity of f-18 fch pet/ct for localization of hyperfunctioning parathyroid gland.At present, the literature regarding the use of f-18 fch pet/ct for localization of hyperfunctioning parathyroid gland remains still limited; thus, further large multicenter studies would be necessary to substantiate the diagnostic accuracy of f-18 fch pet/for localization of hyperfunctioning parathyroid gland in patients with hpt.

Journal: Hormones & cancer (2018)
Publication types: Journal Article;Meta-Analysis;Systematic Review
Possible synergistic effect of apoE and LRP1 genotypes on metabolic syndrome development in Serbian patients.

The modern way of life contributes to the higher frequency of a complex state medically called metabolic syndrome (met s), which is an inevitable consequence of several most common diseases of modern civilization.Patients with met s have three times higher risk of experiencing a heart attack or a stroke and twice higher possibility to die from them.Serbia holds the infamous third place in europe in mortality from heart disease, just behind russia and ukraine.The study explores the correlation of every combination of genotypes of apo e (apolipoprotein e) and lrp1 (low density receptor- related protein 1) genes with presence of met s, and the connection with each anthropometric and biochemical parameter in both tested groups.Study demonstrates the impact of genotype combinations on the emergence and development of the met s in serbia.63 patients and 30 controls were included in the study, aged from 19 to 65.Each person genotype was determined by the polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) profile.Odds ratio (or) values showed that the presence of apo e e3e4/lrp1 cc genotype combination of genotypes in patients multiplies the chance (7.6 times) for the occurrence of the met s in comparison to the presence of other genotype combinations.Determining the genetic basis of met s is one of the necessary steps in the prevention of disease, saving the cost of treatment, and in the design of targeted therapies.

Journal: Molecular biology reports (2019)
Publication types: Journal Article
Recurrent Neoantigens in Colorectal Cancer as Potential Immunotherapy Targets.

This study was aimed at investigating the mutations in colorectal cancer (crc) for recurrent neoantigen identification.A total of 1779 samples with whole exome sequencing (wes) data were obtained from 7 published crc cohorts.Common hla genotypes were used to predict the probability of neoantigens at high-frequency mutants in the dataset.Based on the wes data, we not only obtained the most comprehensive crc mutation landscape so far but also found 1550 mutations which could be identified in at least 5 patients, including krasg12d (8%),krasg12v (5.8%),pik3cae545k (3.5%),pik3cah1047r (2.5%), and bmpr2n583tfs∗44 (2.8%).These mutations can also be recognized by multiple common hla molecules in chinese and tcga cohort as potential "public" neoantigens.Many of these mutations also have high mutation rates in metastatic pan-cancers, suggesting their value as therapeutic targets in different cancer types.Overall, our analysis provides recurrent neoantigens as potential cancer immunotherapy targets.

Journal: BioMed research international (2020)
Publication types: Journal Article
Immunoglobulin Disorders and the Oral Cavity: A Narrative Review.

The oral mucosa is a mechanical barrier against the penetration and colonization of microorganisms.Oral homeostasis is maintained by congenital and adaptive systems in conjunction with normal oral flora and an intact oral mucosa.Components contributing to the defense of the oral cavity include the salivary glands, innate antimicrobial proteins of saliva, plasma proteins, circulating white blood cells, keratinocyte products of the oral mucosa, and gingival crevicular fluid.General disturbances in the level of immunoglobulins in the human body may be manifested as pathological lesions in the oral mucosa.Symptoms of immunoglobulin-related general diseases such as mucous membrane pemphigoid (mmp), pemphigus vulgaris (pv), linear ig a bullous dermatosis (labd), epidermolysis bullosa aquisita (eba), and hyper-ig e syndrome (hies) may appear in the oral cavity.In this review, authors present selected diseases associated with immunoglobulins in which the lesions appear in the oral cavity.Early detection and treatment of autoimmune diseases, sometimes showing a severe evolution (e.g., pv), allow the control of their dissemination and involvement of skin or other body organs.Immunoglobulin disorders with oral manifestations are not common, but knowledge, differentiation and diagnosis are essential for proper treatment.

Journal: Journal of clinical medicine (2022)
Publication types: Journal Article;Review
Opposite forms of adaptation in mouse visual cortex are controlled by distinct inhibitory microcircuits.

Sensory processing in the cortex adapts to the history of stimulation but the mechanisms are not understood.Imaging the primary visual cortex of mice we find here that an increase in stimulus contrast is not followed by a simple decrease in gain of pyramidal cells; as many cells increase gain to improve detection of a subsequent decrease in contrast.Depressing and sensitizing forms of adaptation also occur in different types of interneurons (pv, sst and vip) and the net effect within individual pyramidal cells reflects the balance of pv inputs, driving depression, and a subset of sst interneurons driving sensitization.Changes in internal state associated with locomotion increase gain across the population of pyramidal cells while maintaining the balance between these opposite forms of plasticity, consistent with activation of both vip->sst and sst->pv disinhibitory pathways.These results reveal how different inhibitory microcircuits adjust the gain of pyramidal cells signalling changes in stimulus strength.

Journal: Nature communications (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Lingering Dynamics in Microvascular Blood Flow.

The microvascular networks in the body of vertebrates consist of the smallest vessels such as arterioles, capillaries, and venules.The flow of red blood cells (rbcs) through these networks ensures the gas exchange in as well as the transport of nutrients to the tissues.Any alterations in this blood flow may have severe implications on the health state.Because the vessels in these networks obey dimensions similar to the diameter of rbcs, dynamic effects on the cellular scale play a key role.The steady progression in the numerical modeling of rbcs, even in complex networks, has led to novel findings in the field of hemodynamics, especially concerning the impact and the dynamics of lingering events when a cell meets a branch of the network.However, these results are yet to be matched by a detailed analysis of the lingering experiments in vivo.To quantify this lingering effect in in vivo experiments, this study analyzes branching vessels in the microvasculature of syrian golden hamsters via intravital microscopy and the use of an implanted dorsal skinfold chamber.It also presents a detailed analysis of these lingering effects of cells at the apex of bifurcating vessels, affecting the temporal distribution of plasmatic zones of blood flow in the branches and even causing a partial blockage in severe cases.

Journal: Biophysical journal (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Nomogram for prediction of prolonged postoperative ileus after colorectal resection.

Prolonged postoperative ileus (ppoi) is a major complication in patients undergoing colorectal resection.The aim of this study was to analyze the risk factors contributing to ppoi, and to develop an effective nomogram to determine the risks of this population.Methods a total of 1,254 patients with colorectal cancer who underwent radical colorectal resection at fujian cancer hospital from march 2016 to august 2021 were enrolled as a training cohort in this study.Univariate analysis and multivariate logistic regressions were performed to determine the correlation between ppoi and clinicopathological characteristics.A nomogram predicting the incidence of ppoi was constructed.The cohort of 153 patients from fujian provincial hospital were enrolled as a validation cohort.Internal and external validations were used to evaluate the prediction ability by area under the receiver operating characteristic curve (auc) and a calibration plot.Results in the training cohort, 128 patients (10.2%) had ppoi after colorectal resection.The independent predictive factors of ppoi were identified, and included gender, age, surgical approach and intraoperative fluid overload.The auc of nomogram were 0.779 (95% ci: 0.736-0.822) and 0.791 (95%ci: 0.677-0.905) in the training and validation cohort, respectively.The two cohorts of calibration plots showed a good consistency between nomogram prediction and actual observation.Conclusions a highly accurate nomogram was developed and validated in this study, which can be used to provide individual prediction of ppoi in patients after colorectal resection, and this predictive power can potentially assist surgeons to make the optimal treatment decisions.

Journal: BMC cancer (2022)
Publication types: Journal Article
Ecotoxicological and genotoxic effects of dimethyl phthalate (DMP) on Lemna minor L. and Spirodela polyrhiza (L.) Schleid. plants under a short-term laboratory assay.

The environmental occurrence of phthalates (pae) is of great concern for the ecosystem and human health.Despite of their recognized toxicity on biota, a lack of knowledge is still present about the effects of pae on plants.In this scenario, the effects of dimethyl phthalate (dmp) on duckweed plants (lemna minor l.And spirodela polyrhiza (l.) schleid.), two model plant species for ecotoxicological and trophic studies, were investigated.Under a 7-day lab assay, morphological (biometric indicators), physiological (pigment content and photosynthetic performance) and molecular (dna damage) parameters were studied.No effects were observed at growth and physiological level in both plants at 3 and 30 mg/l dmp.On the contrary, at 600 mg/l dmp, a concentration used for plant acute toxicity studies, a remarkable growth inhibition and pigment content and photosynthetic parameters reduction compared to control were observed in both plants species, particularly in spirodela.Alkaline comet assay in 24 h-treated plants revealed a genotoxic damage induced by dmp, particularly relevant in spirodela.These results described for the first time the adverse effects exerted by dmp on aquatic plants, contributing to highlight the environmental risk associated to the presence of this compound in the aquatic ecosystem.

Journal: The Science of the total environment (2022)
Publication types: Journal Article
Psychiatric training program during the COVID-19 pandemic: An experience in Iran.

This study aims to report a redesigned psychiatric training program in the internship and the externship courses at tehran university of medical sciences, in iran during the covid-19 pandemic.Methods it is a retrospective cohort study which 531 externs and 381 interns enrolled.The students' satisfaction and their exam scores were assessed before and during the covid-19 pandemic.Results blended learning was used for clinical education which theoretical, case-based discussions; and assessments were provided online.Externs' clinical scores and satisfaction were significantly higher while interns' scores got worse.Conclusion e-learning can be effective, applicable and acceptable in clinical education.

Journal: Asian journal of psychiatry (2022)
Publication types: Journal Article
The relationship between empathic concern and perceived personal costs for helping and how it is affected by similarity perceptions.

One explanation for the positive effect of state empathic concern on helping is that such other-focused feelings reduce helpers' perceptions of their personal costs for helping.Results from an experiment (n= 186) supported these assumptions and showed further that self-focused feelings of personal distress, another form of affective empathy, were a positive predictor of perceived costs.Moreover, i examined whether the strength of the negative relationship between empathic concern and personal costs depends on two forms of perceived similarity between the helper and the target, person similarity and experience similarity.For this purpose, i manipulated person similarity by portraying the target as either similar or dissimilar with regard to essential characteristics, and assessed experience similarity by asking whether or not participants share the target's negative experience.As predicted, the negative relationship between empathic concern and perceived personal costs was strongest when person similarity was high and experience similarity low.

Journal: The Journal of social psychology (2022)
Publication types: Journal Article
Injectable Adhesive Self-Healing Multicross-Linked Double-Network Hydrogel Facilitates Full-Thickness Skin Wound Healing.

The development of natural polymer-based hydrogels, combining outstanding injectability, self-healing, and tissue adhesion, with mechanical performance, able to facilitate full-thickness skin wound healing, remains challenging.We have developed an injectable micellar hydrogel (af127/ha-adh/oha-dop) with outstanding adhesive and self-healing properties able to accelerate full-thickness skin wound healing.Dopamine-functionalized oxidized hyaluronic acid (oha-dop), adipic acid dihydrazide-modified ha (ha-adh), and aldehyde-terminated pluronic f127 (af127) were employed as polymer backbones.They were cross-linkedin situusing schiff base dynamic covalent bonds (af127 micelle/ha-adh network and ha-adh/oha-dop network), hydrogen bonding, and π-π stacking interactions.The resulting multicross-linked double-network design forms a micellar hydrogel.The unique multicross-linked double-network structure endows the hydrogel with both improved injection abilities and mechanical performance while self-healing faster than single-network hydrogels.Inspired by mussel foot adhesive protein, oha-dop mimics the catechol groups seen in mussel proteins, endowing hydrogels with robust adhesion properties.We also demonstrate the potential of our hydrogels to accelerate full-thickness cutaneous wound closure and improve skin regeneration with reduced scarring.We anticipate that our hydrogel platform based on a novel multicross-linked double-network design will transform the future development of multifunctional wound dressings.

Journal: ACS applied materials & interfaces (2020)
Publication types: Journal Article