syedzayyan/paper_mill_benchmark huggingface dataset:
HLA-G3'UTR polymorphism diplotypes and soluble HLA-G plasma levels impact cervical cancer susceptibility and prognosis.

Human leukocyte antigen g (hla-g) is an immune checkpoint molecule with relevance in several cancers.The aim of this study was to evaluate the potential role of soluble hla-g (s hla-g), its genetic polymorphisms and its haplotype structure in the susceptibility and prognosis of primary cervical cancer in a chinese han population.Methods we investigated s hla-g plasma levels and 3' untranslated region (3'utr) polymorphisms through elisa and direct dna sequencing, respectively, in cervical cancer patients (120 cases) and healthy control women (96 cases).The data were analyzed for associations using power marker, haploview, and graph pad prism.Results in this study, 8 polymorphic sites, 16 haplotypes and 23 diplotypes in the hla-g3'utr were identified in our study population.We observed that each pair of 8 polymorphic sites exhibited linkage disequilibrium.The heterozygote ct genotype at position +3422 (rs17875408) was more common in cervical cancer patients than in healthy women (or=5.285,p 0.7).Among patients, mean s hla-g levels increased with increasing figo stages but were not related to the overall survival time.Conclusions the results of the present study enhance our understanding of how hla-g3'utr polymorphisms can influence the peripheral s hla-g plasma level and play a key role in cervical carcinogenesis.This study further confirmed that s hla-g may represent a novel plasma biomarker for the prognosis and potential therapeutic target of cervical cancer.

Journal: Frontiers in immunology (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
The Role of TREK-1 and AQP5 in Gonadocorticoid-Related Voice Disorders.

S twik-related potassium channel-1 (trek-1) and aquaporin 5 (aqp5) are involved in epithelial integrity and fluid transport, respectively.In this study, we aimed to compare physiological and gestational patterns of trek-1 and aqp5 location and expression in rat larynx.Our secondary objective was to reveal the effect of estradiol (e2) and progesterone (pg) on these two biomolecules.Methods this study was conducted on 20 wister albino female rats which were assigned as control (group a) and pregnant group (group b).The rats were sacrificed at 20th day of pregnancy.Blood was obtained directly from the ventricle for detection of serum e2 and pg levels.Larynx was resected for immunohistochemical analyses and real-time polymerase chain reaction testing for detection of trek-1 and aqp5 staining and expression, respectively.Results relative trek-1 (p = 0.035) and aqp5 (p = 0.019) expression was found to be significantly high in group b when compared with group a.We found positive correlation between serum e2 levels and both biomolecules (trek-1; p = 0.018, aqp5; p = 0.016).We also found positive correlation between serum pg levels and both biomolecules (trek-1; p = 0.001, aqp5; p = 0.019).Trek-1 immunostaining was found to be higher in surface epithelium and lamina propria of vocal cord mucosa.Aqp5 was particularly found to be located in basement membrane and adjacent superficial lamina propria.We revealed the physiological and gestational pattern of laryngeal trek-1 and aqp5 expression for the first time.Gestational expression of both trek-1 and aqp5 was found to be increased.Stimulatory effect of e2 and pg on laryngeal trek-1 and aqp5 expression was also revealed.Conclusions we revealed upregulatory effect of e2 and pg on laryngeal trek-1 and aqp5 expression.Based on this finding, it can be suggested that trek-1 and aqp5 play role in biomolecular processes leading gonadocorticoid-related voice changes.

Journal: Journal of voice : official journal of the Voice Foundation (2022)
Publication types: Journal Article
Factors associated with balance impairments in the community-dwelling elderly in urban China.

Identification of factors relevant to balance performance impairments in the elderly population was critical for developing effective interventions and preventions.However, there have been very limited data available based on large scale studies.The present study identified factors that independently contributed to performance impairments in overall balance, domains of static balance, postural stability, and dynamic balance, and individual items.Methods a total of 1984 community-dwelling chinese elderly from urban areas of shanghai were recruited.Information on demographic characteristic, exercise, and health status were collected with a face-to-face interview.Balance performances were assessed on site by trained investigators based on the x16 balance testing scale.To identify the effectors, ordinal logistic regression analysis was applied for overall balance, static balance, postural stability, and dynamic balance.Binary logistic regression analysis was used for 16 items.Results the community-dwelling elderly residents were aged from 60 to 97 years old.With increases of age, risks of impairments in overall balance increased gradually (ors from 1.26 to 3.20, all p < 0.01).In the elderly with overweight and obesity, there was higher proportion of balance impairments compared to the elderly with normal bmi (or = 1.26, p < 0.001).Regular exercise every week was associated with reduced risks of balance impairments (ors from 0.63 to 0.73, all p < 0.001).Presences with vision lesion (ors from 1.28 to 1.59, all p < 0.001), moderate hearing impairment (or = 1.54, p < 0.001), somesthesis dysfunction (ors from 1.59 to 13.26, all p < 0.001), and cerebrovascular disease (or = 1.45, p = 0.001) were related to increased risks of balance impairments.Likewise, age, exercise, vision, hearing, somesthesis, and cerebrovascular disease were significantly associated with static balance, postural stability, and dynamic balance.Both overweight and obesity and underweight were associated with higher proportions of dynamic balance impairments.Regular exercise was significantly related to reduced risks of impairments in 15 out of the 16 items.Conclusions in the elderly, age, overweight and obesity, exercise, vision, hearing, somesthesia, and cerebrovascular disease were dominant factors associated with impairments in overall balance, domains of static balance, postural stability, and dynamic balance, and most individual items.Trial registration not applicable.

Journal: BMC geriatrics (2023)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Theory and Least Squares Fitting of CW ESR Saturation Spectra Using the MOMD Model.

Cw saturation experiments are widely used in esr studies of relaxation processes in proteins and lipids.We develop the theory of saturation in esr spectra in terms of its close relation with that of 2d-eldor.Our treatment of saturation is then based on the microscopic order macroscopic disorder (momd) model and can be used to fit the full cw saturation spectrum, rather than fitting just the peak-peak amplitude as a function of microwave field b1as is commonly done.This requires fewer experiments to yield effects on t1, as well as provides a more extensive dynamic structural picture, for example, for scanning experiments on different protein sites.The code is released as a publicly available software package in python that can be used to fit cw saturation spectra from biological samples of interest.

Journal: Applied magnetic resonance (2022)
Publication types: Journal Article
Development of a Novel Immune-Related Gene Signature to Predict Prognosis and Immunotherapeutic Efficiency in Gastric Cancer.

Gastric cancer (gc) is the fifth most common malignancy and the third leading cause of tumor-related deaths globally.Herein, we attempted to build a novel immune-related gene (irg) signature that could predict the prognosis and immunotherapeutic efficiency for gc patients.methods:the m rna transcription data and corresponding clinical data of gc were downloaded from the cancer genome atlas (tcga) database as the training group and the gse84437 data set as the testing cohort, followed by acquisition of irgs from the innate db resource and imm port database.Using the univariate cox regression analysis, an irg signature was developed.Several immunogenomic analyses were performed to illustrate the associations between the immune risk score and tumor mutational burden, immune cell infiltrations, function of immune infiltration, clinical characteristics, immune subtype, and immunotherapeutic response.results:the analysis of 343 gc samples and 30 normal samples from the tcga database gave rise to 8,713 differentially expressed genes (degs) and 513 differentially expressed immune-related genes (deirgs) were extracted.The novel irg signature contained eight deirgs (fabp4, pi15, rnase2, cgb5, inhbe, rln2, dusp1, and cd36) and was found to serve as an independent predictive and prognostic factor for gc.Then, the gc patients were separated into the high- and low-risk groups based on the median risk score, wherein the low-risk group presented a better prognosis and was more sensitive to immunotherapy than did the high-risk group.According to the time-dependent roc curves and aucs, the immunotherapeutic value of the signature was better than the tumor immune dysfunction and exclusion (tide) and t-cell inflammatory signature (tis) scores.In addition, the aucs of the risk score for predicting 1-, 2-, and 3-year os were 0.675, 0.682, and 0.710, respectively, which indicated that the signature had great predictive power.conclusion:this study presents a novel irg signature based on the tumor immune microenvironment, which could improve the prediction of the prognosis and immunotherapeutic efficiency for gc patients.The powerful signature may serve as novel biomarkers and provide therapeutic targets for precision oncology in clinical practice.

Journal: Frontiers in genetics (2022)
Publication types: Journal Article
Ground Calcium Carbonate as a Low Cost and Biosafety Excipient for Solubility and Dissolution Improvement of Praziquantel.

Calcium carbonate is an abundant mineral with several advantages to be a successful carrier to improve oral bioavailability of poorly water-soluble drugs, such as praziquantel.Praziquantel is an antiparasitic drug classified in group ii of the biopharmaceutical classification system hence characterized by high-permeability and low-solubility.Therefore, the dissolution rate is the limiting factor for the gastrointestinal absorption that contributes to the low bioavailability.Consequently, the therapeutic dose of the praziquantel must be high and big tablets and capsules are required, which are difficult to swallow, especially for pediatric and elderly patients.Mixtures of praziquantel and calcium carbonate using solid-solid physical mixtures and solid dispersions were prepared and characterized using several techniques (x-ray diffraction differential scanning calorimetry, thermogravimetric analysis, scanning electron microscopy, laser diffraction, fourier transform infrared and raman spectroscopies).Solubility of these formulations evidenced that the solubility of praziquantel-calcium carbonate interaction product increased in physiological media.in vitrodissolution tests showed that the interaction product increased the dissolution rate of the drug in acidic medium.Theoretical models were studied to understand this experimental behavior.Cytotoxicity and cell cycle studies were performed, showing that praziquantel-calcium carbonate physical mixture and interaction product were biocompatible with the htc116 cells, because it did not produce a decrease in cell viability or alterations in the cell cycle.

Journal: Pharmaceutics (2019)
Publication types: Journal Article
The interaction network of the YidC insertase with the SecYEG translocon, SRP and the SRP receptor FtsY.

Yid c/oxa1/alb3 are essential proteins that operate independently or cooperatively with the sec machinery during membrane protein insertion in bacteria, archaea and eukaryotic organelles.Although the interaction between the bacterial sec yeg translocon and yid c has been observed in multiple studies, it is still unknown which domains of yid c are in contact with the sec yeg translocon.By in vivo and in vitro site-directed and para-formaldehyde cross-linking we identified the auxiliary transmembrane domain 1 of e.Coli yid c as a major contact site for sec y and sec g.Additional sec y contacts were observed for the tightly packed globular domain and the c1 loop of yid c, which reveals that the hydrophilic cavity of yid c faces the lateral gate of sec y.Surprisingly, yid c-sec yeg contacts were only observed when yid c and sec yeg were present at about stoichiometric concentrations, suggesting that the yid c-sec yeg contact in vivo is either very transient or only observed for a very small sec yeg sub-population.This is different for the yid c-srp and yid c-fts y interaction, which involves the c1 loop of yid c and is efficiently observed even at sub-stoichiometric concentrations of srp/fts y.In summary, our data provide a first detailed view on how yid c interacts with the sec yeg translocon and the srp-targeting machinery.

Journal: Scientific reports (2018)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Antimicrobial peptide GL13K immobilized onto SLA-treated titanium by silanization: antibacterial effect against methicillin-resistantStaphylococcus aureus(MRSA).

Infection is the main reason for implant failure, and the incidence of drug-resistant bacterial infection has increased in recent years.Further, methicillin-resistant staphylococcus aureus(mrsa)-related implant infection has become a serious worldwide threat.New strategies, other than antibiotics, to tackle drug-resistance, are of high clinical significance.Antimicrobial peptides show clear superiority over conventional antibiotics in inhibiting drug-resistant bacteria.In the present study, we combined the antimicrobial peptide, gl13k, with sandblasting and acid-etching (sla)-treated titanium using a silane coupling agent.Field emission scanning electron microscopy images showed the morphology of the coating.Attenuated total reflectance fourier transform infrared spectroscopy and x-ray photoelectron spectroscopy results confirmed loading of gl13k, and the hydrophilicity of the sla-gl13k coating was evaluated by water contact angle analysis.The releasing study of samples showed that the coating has a sustained releasing profile.Sla-gl13k coating exhibited strong contact- and release-killing abilities against mrsa,e.Coli, and s.Aureus.Meanwhile, cell counting kit 8 analysis and examination of cell morphology demonstrated that the sla-gl13k coating had good cytocompatibility at antibacterial concentrations.Overall, all these results suggest that sla-gl13k coating can be successfully fabricated using silanization, and is a promising candidate for controlling mrsa-induced implant-related infection.

Journal: RSC advances (2022)
Publication types: Journal Article
BRASH Syndrome Presenting With Idioventricular Escape Rhythm in a Patient With Trifascicular Block.

Bradycardia, renal failure, atrioventricular (av) nodal disease, shock, and hyperkalemia (brash) syndrome is a well-recognized constellation of distinct clinicopathologic entities comprising bradycardia, renal failure, av nodal disease, shock, and hyperkalemia.Our patient is an 89-year-old female with a past medical history significant for hypertension and diabetes, who was newly started on labetalol and had recent gastroenteritis; she presented to our emergency department with bradycardia and shock.Upon presentation, she showed physical signs of volume depletion, and her blood pressure was 50 mm hg systolic and heart rate was 25 beats per minute.The initial electrocardiogram showed an idioventricular rhythm.The laboratory workup revealed hyperkalemia.The patient was given repeated doses of atropine with no significant response.She was resuscitated with isotonic fluids.The patient improved clinically, her blood pressure stabilized, her potassium level, renal function, and heart rate were normalized, and normal sinus rhythm was restored with a narrow qrs complex.A diagnosis of brash syndrome was made retrospectively.Overall, the treatment of this syndrome is largely symptomatic.Hemodynamic support with fluid and treatment of hyperkalemia remains the goal of care.The overall prognosis is good if identified early and managed appropriately.

Journal: Cureus (2022)
Publication types: Case Reports;Journal Article
The "Corona Warriors"? Community health workers in the governance of India's COVID-19 response.

India's nearly 1-million strong band of quasi-volunteer accredited social health activists (ashas) have been key actors in government efforts to control covid-19.Utilizing a nationalist rhetoric of war, ashas were swiftly mobilized by the government in march 2020 as 'covid warriors' engaged in tracking illness, disseminating information, and caring for quarantined individuals.The speed at which ashas were mobilized into mentally and physically grueling labor was all the more stunning given these minimally paid community health workers have long been seen to have low morale given their precarious, informalized work arrangements.Building on work examining the spatialities of global health governance alongside literature on geographic contingency, this paper explores the ways that nationalist covid-19 war rhetoric promulgated from delhi worked as a technology of health governance to propel ashas into certain forms of action, yet also opened up spaces of potentiality for them to reimagine their relationship to both the state and the communities they serve.In particular, in our analysis of in-depth telephone interviews with asha workers in the state of himachal pradesh, we find that their hailing as covid warriors inspired patriotic calls to duty and legitimized their (long over-looked) roles as critical governance actors, yet also was subject to resistance and reworking due to a combination of institutional histories, local politics, as well as happenstantial everyday encounters of asha work.The precarious employment of ashas - in terms of basic remuneration as well as the great on-the-job risks that they have faced - underscores both the fragile nature of india's health governance system as well as possible political movements for its renewal.We conclude by calling for geographers to give greater attention to community health care workers as a key window into understanding the uneven ways in which health systems are made manifest on the ground, and their ability to respond to citizens' healthcare needs - both in the covid-19 pandemic and beyond.

Journal: Political geography (2022)
Publication types: Journal Article
Vascular Injury in the Zebrafish Tail Modulates Blood Flow and Peak Wall Shear Stress to Restore Embryonic Circular Network.

Mechano-responsive signaling pathways enable blood vessels within a connected network to structurally adapt to partition of blood flow between organ systems.Wall shear stress (wss) modulates endothelial cell proliferation and arteriovenous specification.Here, we study vascular regeneration in a zebrafish model by using tail amputation to disrupt the embryonic circulatory loop (ecl) at 3 days post fertilization (dpf).We observed a local increase in blood flow and peak wss in the segmental artery (se a) immediately adjacent to the amputation site.By manipulating blood flow and wss via changes in blood viscosity and myocardial contractility, we show that the angiogenic notch-ephrinb2 cascade is hemodynamically activated in the se a to guide arteriogenesis and network reconnection.Taken together, ecl amputation induces changes in microvascular topology to partition blood flow and increase wss-mediated notch-ephrinb2 pathway, promoting new vascular arterial loop formation and restoring microcirculation.

Journal: Frontiers in cardiovascular medicine (2022)
Publication types: Journal Article
One-Shot Synthesis and Melt Self-Assembly of Bottlebrush Copolymers with a Gradient Compositional Profile.

Morphological control plays a central role in soft materials design.Herein, we report the synthesis of a gradient bottlebrush architecture and its role in directing molecular packing in the solid state.Bottlebrush copolymers with gradient interfaces were prepared via one-shot ring-opening metathesis polymerization ofexo- andendo-norbornene-capped macromonomers.Kinetic studies revealed a gradient compositional profile separating the two blocks along the backbone.Side-chain symmetric gradient bottlebrush copolymers exhibited a strong tendency to assemble into cylindrical microstructures, in contrast to their block copolymer analogs with sharp interfaces.Such exquisite architectural control of the interfacial composition affords a delicate handle to direct macromolecular assembly.

Journal: ACS macro letters (2018)
Publication types: Journal Article
A carboxylatopillar[5]arene-based pH-triggering supramolecular photosensitizer for enhanced photodynamic antibacterial efficacy.

A p h-triggering supramolecular antibacterial photosensitizer was constructed by host-guest interaction between a water-soluble porphyrin photosensitizer and carboxylatopillar[5]arene (p[5]).The formation of the supramolecular complex not only improves the biocompatibility of the photosensitizer, but also enhances antibacterial efficacy by p h-triggering dissociation under the low p h bacterial microenvironment.

Journal: Chemical communications (Cambridge, England) (2022)
Publication types: Journal Article
Benefits of Airway Androgen Receptor Expression in Human Asthma.

Rationale:androgens are potentially beneficial in asthma, but ar(androgen receptor) has not been studied in human airways.objectives:to measure whether arand its ligands are associated with human asthma outcomes.methods:we compared the effects of arexpression on lung function, symptom scores, and fractional exhaled nitric oxide (fe no) in adults enrolled in sarp (severe asthma research program).The impact of sex and of androgens on asthma outcomes was also evaluated in the sarp with validation studies in the cleveland clinic health system and the nhanes (u.s.National health and nutrition examination survey).measurements and main results:in sarp (n= 128),argene expression from bronchoscopic epithelial brushings was positively associated with both fev1/fvc ratio (r2= 0.135,p= 0.0002) and the total asthma quality of life questionnaire score (r2= 0.056,p= 0.016) and was negatively associated with fe no(r2= 0.178,p= 9.8 × 10-6) and nos2(nitric oxide synthase gene) expression (r2= 0.281,p= 1.2 × 10-10).In sarp (n= 1,659), the cleveland clinic health system (n= 32,527), and the nhanes (n= 2,629), women had more asthma exacerbations and emergency department visits than men.The levels of the arligand precursor dehydroepiandrosterone sulfate correlated positively with the fev1in both women and men.conclusions:higher bronchial arexpression and higher androgen levels are associated with better lung function, fewer symptoms, and a lower fe noin human asthma.The role of androgens should be considered in asthma management.

Journal: American journal of respiratory and critical care medicine (2021)
Publication types: Journal Article;Research Support, N.I.H., Extramural