syedzayyan/paper_mill_benchmark huggingface dataset:
miR-223-5p Suppresses OTX1 to Mediate Malignant Progression of Lung Squamous Cell Carcinoma Cells

Lung squamous cell carcinoma (lusc) features high morbidity and mortality as a worldwide malignant tumor.This study mainly explored a mi r-223-5p-dependent mechanism that affected proliferation, invasion, and migration of lusc cells.Methods.Expression data of mature mi rnas and sequencing data of total rna of lusc were downloaded from tcga database.Differentially expressed m rnas were obtained.Function of mi r-223-5p in lusc cells was detected by assays like q rt-pcr, mtt, wound healing assay, western blot, and transwell assay.Western blot was performed to analyze the relationship between otx1 and jak/stat signaling pathways.Dual-luciferase assay detected the relationship between mi r-223-5p and otx1.The way how mi r-223-5p regulated lusc cell biological functions via otx1 was further explored.Results.It was noted that mi r-223-5p expression in lusc tissue and cells was significantly reduced.Overexpression of mi r-223-5p negatively regulated the proliferation, invasion, and migration of lusc cells.The downstream target gene otx1 was detected to be notably elevated in lusc cells.A negative correlation between otx1 and mi r-223-5p was also found.As analyzed by gsea, otx1 was significantly enriched in the jak/stat signaling pathway and activated the pathway.Dual-luciferase assay demonstrated that otx1 was a direct molecular target of mi r-223-5p in lusc cells.Rescue experiment verified that mi r-223-5p regulated the malignant phenotypes of lusc cells by pairing with otx1.Conclusion.This study indicated that mi r-223-5p was lowly expressed in lusc cells.The impact of mi r-223-5p on cell proliferation, invasion, and migration was realized by targeting otx1.It is likely that mi r-223-5p can be a novel target for lusc treatment, which provides new ideas for future lusc treatment.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Research Article;
The Investigation of Pulmonary Function Changes of COVID-19 Patients in Three Months

Novel coronavirus disease 2019 (covid-19) was discovered in december 2019 and has infected more than 80 million people worldwide, and more than 50 million people have achieved a clinical cure.In this study, the pulmonary function results of patients after clinical medicine for three months were reported.Objective.To investigate the effect of covid-19 on lung function in patients.Methods.A retrospective analysis was performed on 56 covid-19-infected patients who were cured after the clinical treatment at taizhou public health medical center in zhejiang province from january 31, 2020, to march 10, 2020.At discharge and three months after discharge, lung function was measured, including inspiratory vital capacity (ivc), forced vital capacity (fvc), forced expiratory volume in first second (fev1), forced expiratory volume in first second to inspiratory vital capacity (fev1/ivc), maximum mid-expiratory flow rate (mef), peak expiratory flow rate (pef), and carbon monoxide dispersion (dlco).Results.At discharge, there were 37 patients (66.1%) with pulmonary dysfunction, 22 patients (39.3%) with ventilation dysfunction, 31 cases (55.4%) with small airway dysfunction, and 16 cases (28.6%) with restricted ventilation dysfunction combined with small airway dysfunction.At 3 months after discharge, 24 of the 56 patients still had pulmonary dysfunction and all of them had small airway dysfunction, of which 10 patients (17.9%) were restricted ventilation dysfunction combined with small airway dysfunction.Dlco was measured three months after discharge.Twenty-nine patients (51.8%) had mild to moderate diffuse dysfunction.All pulmonary function indexes of 56 patients recovered gradually after 3 months after release, except fev1/ivc, and the difference was statistically significant ( p &lt; 0.05).There were 41 patients of normal type (73.2%) and 15 patients of severe type (26.8%).Among the 15 severe patients, 8 patients (53.3%) had ventilation dysfunction at discharge, 9 patients (60%) had small airway dysfunction, 4 patients (26.7%) still had ventilation dysfunction 3 months after discharge, 7 patients (46.7%) had small airway dysfunction, and 10 patients (66.7%) had diffuse dysfunction.Among the 41 common type patients, 14 patients (34.1%) had ventilation dysfunction at discharge, 22 patients (53.7%) had small airway dysfunction, 6 patients (14.6%) still had ventilation dysfunction 3 months after discharge, 17 patients (41.5%) had small airway dysfunction, and 19 patients (46.3%) had diffuse dysfunction.Patients with severe covid-19 had more pulmonary impairment and improved pulmonary function than normal patients.Conclusion.Covid-19 infection can cause lung function impairment, manifested as restricted ventilation dysfunction, small airway dysfunction, and diffuse dysfunction.The pulmonary function of most patients was improved 3 months after clinical cure and discharge, and some patients remained with mild to moderate diffuse dysfunction and small airway dysfunction.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Static Model of Athlete’s Upper Limb Posture Rehabilitation Training Indexes

With the gradual expansion of the development of sports, the level of sports has been rapidly improved.Athletes have to carry out high‐intensity and systemic technical movements in training and competition.Some sports have the greatest burden on the shoulder joint.From the observation and investigation of the injured parts of athletes, it is found that the shoulder joint is the most common sports injury, which is the most typical sports injury.Based on the problem of insufficient strength and endurance reserve after rehabilitation of shoulder external rotator injury, it will cause muscle tension and poor extensibility.To prove the improvement effect of functional training and posture index calibration on the poor posture of the shoulder, considering the measurement of global passive torque, this paper uses a limited set of joint angles and corresponding passive torque data in the upper arm lifting trajectory to train the neural network and uses the trained network to predict the passive torque in other upper arm trajectories.The kinematics model of the shoulder joint is established, and the human‐computer interaction experiment is designed on the platform of the gesture index manipulator.The passive and active torque components of the shoulder joint in the human‐computer interaction process are calculated by measuring the man‐machine interaction force of the subjects in the motion state, which is used as the basis for evaluating the active motion intention of the subjects.Surface electromyography (semg) was used to calibrate and verify the attitude index of shoulder active torque.The method proposed in this paper is helpful to achieve more efficient on‐demand assisted rehabilitation training exercises, which is of great significance to improve the level of rehabilitation training.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Study on the Evaluation of Lung Cancer Patients from the Three Aspects of Emotion

In recent years, epidemiological survey data have shown that lung cancer is the tumor with the fastest increase in cancer incidence and mortality in china.The incidence and mortality of lung cancer in china rank first among tumors, and 80% of patients die within one year of diagnosis.This paper aims to study the evaluation of lung cancer patients from three aspects of emotion, coping style, and quality of life (qo l), expounding on the changes in emotion, coping style, and qo l in lung cancer patients after chemotherapy.We assess the negative emotions and survival of lung cancer patients after chemotherapy quality satisfaction survey research.We investigate the general data, qo l, and coping styles of 219 lung cancer patients undergoing chemotherapy before, during, and after chemotherapy based on the artificial intelligence processor.All survey data are input into spss 19 for descriptive and relevant statistical analysis.The experimental results show that under a survey of 219 lung cancer chemotherapy patients with negative emotions and qo l satisfaction after chemotherapy, at a significance level of = 0.05, there is a linear regression relationship between mental resilience and anxiety and depression.

Journal: Contrast Media & Molecular Imaging (2022)
Publication types: Research Article;
Risk Management of Sports Venues and Olympic Sports Cooperation Spirit under Complex Environment

The understanding of sports event risk and olympic sports cooperation spirit, as well as the systematic analysis and research of sports event risk management, are still in their early stages in china’s sports theory circle.Institutions for developing sports talent also disregard programmes designed to increase the risk awareness and olympic spirit of sports workers.This essay provides an overview of the concepts of sports event risk and olympic sports cooperation spirit based on reality.This paper examines the interaction of risk management of large‐scale sporting events and olympic sports cooperation spirit from the perspective of event organization managers, using the pertinent theory of risk management and the random forest algorithm in machine learning.The experimental results show that, according to the analysis of injury occurrence according to different positions, it is discovered that forward athletes suffer the most injuries, with an average of 54 forwards in injury situations, followed by guards with an average of 43 guards in injury situations, and finally centres with an average of 38 guards in injury situations.Combining risk management for major sporting events with the cooperative olympic sports spirit has significant practical implications.

Journal: Journal of Environmental and Public Health (2022)
Publication types: Research Article;
Review on Comfort Nursing Interventions for Patients Undergoing Neurosurgery and General Surgery

To summarize the commonalities and particularities of comfort care interventions for neurology and general surgery patients.Methods.The development of comfort care and its practical application in neurology and general surgery were discussed and summarized by searching the current literature on comfort care interventions for neurology and general surgery patients, including case reports, clinical studies, and systematic reviews.Results.Comfort nursing intervention is a kind of nursing intervention with integrity and creativity.In addition to its uniqueness, the comfort nursing model also has strong effectiveness.Clinical holistic nursing has been significantly improved through the application of comfort nursing intervention theory, and its nursing mode has enhanced the connotation of clinical nursing.Conclusion.Comfort nursing intervention for neurology and general surgery patients can help patients recover and deserves further promotion.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Review Article;
Research on the Influence of Physical Exercise Health on the Mental Health of University Students

Exercise has become one of the essential life skills of university students and is also an integral part of physical exercise and mental health.As a positive influence on their physical and mental health, sports can help people to adjust and regulate their emotions and behavior.At the same time, the mental health of university students has been on the decline in recent years.At the university level, students tend to develop anxiety, depression and other emotions and problems, and temptations of all kinds provide a direction for psychological problems to arise.For university educators, the way to improve the mental health of university students is to study the current psychological situation of university students, identify the symptoms, treat them, and adopt scientific and effective interventions while sports are popular among young people as a positive and fun activity.In order to investigate the relationship between sports health and mental health of university students, the author chose thousands of students in a school as the research subjects using experimental methods and mathematical and statistical methods to conduct physical and psychological tests on the research subjects through different comparative experimental content and data results to explore the impact of sports health on the mental health of university students and using professional data analysis software ibm.The data from the physical health assessment and the psychological health assessment obtained from the school assessment center were analyzed by using the independent sample t ‐test and correlation analysis method in spss statistics 19.0, so as to investigate the influence of physical health on psychological health.

Journal: Journal of Environmental and Public Health (2022)
Publication types: Research Article;
Research on Improving Radiotherapy Accuracy Based on Image-Guided Radiotherapy

With the changes of people’s diet and lifestyle, the number of patients with abdominal malignant tumors is increasing year by year.In order to analyze the effectiveness of cone‐beam ct (cbct) enhancement technology in improving the accuracy of radiotherapy for clinical malignant tumors, 92 patients with abdominal malignant tumor are divided into the control group and the cbct radiotherapy group.The experimental results show that precise radiotherapy technology can promote the recovery of the immune function of patients with abdominal malignant tumors, improve the effect of treatment, and decrease the incidence of complications.

Journal: Contrast Media & Molecular Imaging (2022)
Publication types: Research Article;
MiR-192-5p Alleviated Fibrosis and Inflammatory Responses of Tendon Cells by Targeting NFAT5

To explore the effect of micro rna (mi r)-192-5p on the inflammatory and fibrotic responses of tendon cells.Methods.Tendon cells were treated with transforming growth factor-β1 (tgf-β1).The expression of mi r-192-5p and nuclear factor of activated t cells 5 (nfat5) in tendon cells were detected by rt-q pcr.The expressions of inflammatory and fibrosis-related factors were detected by rt-q pcr and western blot.Mi r-192-5p binds to nfat5 targeting by target scan and dual-luciferase reporter gene assay.The expression of the nfat5 gene was detected by rt-q pcr and western blot.Detection of apoptosis in tendon cells by flow cytometry.Results.Mi r-192-5p was downregulated in tendon cells, and the expression level gradually decreased with the prolong of tgf-β1 treatment.The expression of nfat5 increased with the treatment time of tgf-β1.The expression of mi r-192-5p decreased collagen iii (coliii), α smooth muscle actin (α-sma), matrix metalloproteinase- (mmp-) 1, and mmp-8 expression, thereby inhibiting tgf-β1-induced fibrosis in tendon cells.The expression of mi r-192-5p decreased the expression of tumor necrosis factor-α (tnf-α), interleukin (il)-6, and il-1β, thereby alleviating tgf-β1-induced inflammatory response and reduce apoptosis in tendon cells.Nfat5 is a direct target of mi r-192-5p in tendon cells.The upregulation of nfat5 reversed the effect of mi r-192-5p on the fibrotic activity and inflammatory response of tgf-β1-stimulated tendon cells.Conclusions.Mi r-192-5p alleviates fibrosis and inflammatory responses of tendon cells by targeting nfat5.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
miR-182-5p Serves as an Oncogene in Lung Adenocarcinoma through Binding to STARD13

Lung cancer as one of the commonest invasive malignancies is featured by high morbidity and mortality, wherein lung adenocarcinoma (luad) is the most prevalent subtype.Accumulating evidence exhibited that micro rnas are involved in luad occurrence and progression.In this study, mi r-182-5p was observed to increase in both luad tissue and cell lines.Overexpression of mi r-182-5p could prominently facilitate cell proliferation, migration, and invasion in luad.Through bioinformatics analysis, stard13 was theorized as the target gene of mi r-182-5p, which was lowly expressed in luad.Further molecular experiments manifested that mi r-182-5p bound to the 3 ′ -untranslated region of stard13, and there was an inverse correlation between stard13 and mi r-182-5p in luad.Rescue experiments demonstrated that silencing stard13 conspicuously restored the inhibitory effect of decreased mi r-182-5p on cell proliferation, migration, and invasion in luad.Together, our findings revealed novel roles of the mi r-182-5p/stard13 axis in luad progression.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Research Article;
miR-152-3p Affects the Progression of Colon Cancer via the KLF4/IFITM3 Axis

The purpose of this study was to investigate the relationship between mi r-152-3p and the klf4/ifitm3 axis, thereby revealing the mechanism underlying colon cancer occurrence and development, consequently providing a promising target for colon cancer treatment.Methods .Bioinformatics methods were implemented to analyze the differential expression of mi rnas and m rnas in colon cancer, confirm the target mi rna, and predict the downstream targeted m rnas.Q rt-pcr and western blot were performed to detect the expression of mi r-152-3p, klf4, and ifitm3.Cck-8 and colony formation assays were conducted for the assessment of cell proliferation, and flow cytometry was carried out for the detection of cell apoptosis.Finally, dual-luciferase reporter gene assay was employed to verify the targeting relationship between mi r-152-3p and klf4.Results .Mi r-152-3p was highly expressed in colon cancer cells, whereas klf4 was poorly expressed.Dual-luciferase assay verified that mi r-152-3p targeted to bind to klf4 and suppressed its expression.Moreover, silencing mi r-152-3p or overexpressing klf4 was found to downregulate ifitm3, thereby inhibiting cell proliferation and potentiating cell apoptosis.In rescue experiments, we found that mi r-152-3p deficiency decreased the expression of ifitm3 and weakened cancer cell proliferation, and such effects were restored when mi r-152-3p and klf4 were silenced simultaneously.Conclusion .In sum, we discovered that mi r-152-3p can affect the pathogenesis of colon cancer via the klf4/ifitm3 axis.

Journal: Computational and Mathematical Methods in Medicine (2020)
Publication types: Research Article;
MicroRNA-340 and MicroRNA-450b-5p: Plasma Biomarkers for Detection of Non-Small-Cell Lung Cancer

Since the inefficient cancer management is caused by inaccurate diagnoses, there is a need for minimally invasive method to improve the diagnostic accuracy of non‐small‐cell lung (nsclc).This study intended to detect mi r‐340 and mi r‐450b‐5p levels in plasma from nsclc patients and to assess the potential values for the prediction of tumor development and prognosis.Methods .A gse64591 dataset included 200 samples (100 early‐stage nsclc patients and 100 noncancer control) aimed to identify a panel of circulating mi rnas in plasma.The levels of mi r‐340 and mi r‐450b‐5p in plasma from nsclc patients ( n = 120) and healthy controls ( n = 120) were detected by quantitative real‐time polymerase chain reaction (q rt‐pcr).The diagnostic and prognostic value of plasma mi r‐340 and mi r‐450b‐5p were performed using receiver operating curves (roc), kaplan‐meier method, and cox regression analysis.Results .Mi r‐450b‐5p and mi r‐340 in plasma was significant difference between early‐stage nsclc patients and noncancer control by searching the gse64591 dataset.When compared with the healthy controls, the plasma mi r‐340 was decreased in the nsclc patients, but the plasma mi r‐450b‐5p was increased.Nsclc patients could be distinguished accurately from healthy controls by the circulating mi r‐340 and mi r‐450b‐5p with the auc of 0.740 (95% ci: 0.677~0.804) and of 0.808 (95% ci: 0.754~0.861), respectively.With these two markers, the specificity and sensitivity were 78.33% and 77.5% with the auc of 0.862.Patients with advanced t, n, and tnm stage demonstrated lower plasma mi r‐340 and higher plasma mi r‐450b‐5p, and both of them were correlated with the prognosis of nsclc patients.Furthermore, plasma mi r‐340 was also negatively correlated with tumor grade.All clinicopathological variables significantly associated to prognosis were t stage, n stage, tnm stage, tumor grade, and plasma levels of mi r‐340 and mi r‐450b‐5p in univariate cox regression analysis.The variables that retained their significance in the multivariate model were t stage, plasma mi r‐340, and plasma mi r‐450b‐5p.Conclusion .The plasma levels of mi r‐340 combined with mi r‐450b‐5p potentially define core biomarker signatures for improving the accuracy of nsclc diagnosis.Moreover, circulating mi r‐340 and mi r‐450b‐5p are independent biomarkers of survival in nonmetastatic nsclc patients.

Journal: Journal of Environmental and Public Health (2022)
Publication types: Research Article;
MicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A

The study was designed to evaluate the underlying mechanism of micro rna-139-5p in breast cancer (bc).Methods.Expression statuses of micro rna-139-5p and mex3a were measured by q rt-pcr and western blotting.The anticancer effect of micro rna-139-5p in vitro was tested by a set of assays.Interaction between micro rna-139-5p and mex3a was validated by dual-luciferase detection.Results.Micro rna-139-5p expression in bc cells was obviously low, while mex3a was significantly overexpressed.Micro rna-139-5p restrained proliferative, invasive, and migratory abilities of bc cells and increased apoptosis level of bc cells, while mex3a exerted a promoting effect on bc cell growth.Dual-luciferase reporter detection confirmed that micro rna-139-5p bound to mex3a 3 ′ -utr.Conclusions.Micro rna-139-5p inhibited the development of bc by targeting mex3a.Micro rna-139-5p/mex3a may be a target for bc therapy.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Research Article;
Metoprolol Mitigates Ischemic Heart Remodeling and Fibrosis by Increasing the Expression of AKAP5 in Ischemic Heart

The harm of heart failure mainly causes patients to develop dyspnea, fatigue, fluid retention, and other symptoms, which impair patients’ activity tolerance and lead to a dramatic decrease in patients’ quality of life.The purpose of this study was to verify whether metoprolol regulates akap5 expression and test the role of akap5 postinjury in mitigating cardiac infarction-associated tissue remodeling and fibrosis.Sprague-dawley (sd) rats underwent coronary artery ligation (cal), which was followed immediately with metoprolol daily.And western blot and coimmunoprecipitation experiments were performed to detect the expression of related proteins in the sham-operated group, model group, and drug-treated group.Hw/bw ratio and cardiac expression of col1 and col3 were increased in rats following cal compared with shams.Treatment with metoprolol postinjury was associated with a decrease in hw/bw ratio and col1/col3 expression compared to uncontrolled rats.Cal resulted in decreased cardiac akap5 expression compared to the control group, while metoprolol treatment restored levels compared to baseline shams.Cardiac expression levels of nfatc3/p-nfatc3 and gata4 were modest at baseline and increased with injury, whereas metoprolol suppressed gene expression to below injury-associated changes.Immunoprecipitation indicated that akap5 could bind and regulate pp2b.In summary, we know that metoprolol alleviates ischemic cardiac remodeling and fibrosis, and the mechanism of alleviating remodeling may improve cardiac akap5 expression and akap5-pp2b interaction.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;