syedzayyan/paper_mill_benchmark huggingface dataset:
Can a knee sleeve influence ground reaction forces and knee joint power during a step-down hop in participants following anterior cruciate ligament reconstruction? A secondary analysis.

Elastic knee sleeves are often worn following anterior cruciate ligament (acl) reconstruction.The study aimed to define immediate and 6-week effects of wearing a knee sleeve on ground reaction forces (grf) and knee joint power during a step-down hop task.Methods using a cross-over design, we estimated grf and knee kinematics and kinetics during a step-down hop for 30 participants following acl reconstruction (median 16 months post-surgery) with and without wearing a knee sleeve.In a subsequent randomised clinical trial, participants in the 'sleeve group' (n = 9) wore the sleeve for 6 weeks at least 1 hour daily, while a 'control group' (n = 9) did not wear the sleeve.We compared the following outcomes using statistical parametric mapping (spm): (1) grf and knee joint power trajectories between three conditions at baseline (uninjured side, unsleeved injured and sleeved injured side); (2) grf and knee joint power trajectories within-participant changes from baseline to follow-up between groups.We also compared discrete peak grfs and power, rate of (vertical) force development, and mean knee joint power in the first 5% of stance phase.Results spm showed no differences for grf for the (unsleeved) injured compared to the uninjured sides; when wearing the sleeve, injured side mean power in the first 5% of stance increased significantly from a concentric to an eccentric power.Discrete variables showed lower peak anterior (propulsive) grf, mean power in the first 5% of stance, peak eccentric and concentric power for the injured compared to the uninjured sides.After six weeks, a directional change for vertical grf differed showed slightly decreased forces for the control group and increased forces for the sleeve group.Conclusion wearing a knee sleeve on the anterior cruciate ligament injured knee improved knee power during the first 5% of stance during the step-down hop.No consistent changes were observed for ground reaction forces for spm and discrete variable analyses.Wearing the knee sleeve at least one hour daily for 6-weeks lead to a directional change of increased vertical grf for the sleeve group at follow-up.Trial registration the trial was prospectively registered with the australia new zealand clinical trials registry no: actrn12618001083280, 28/06/2018.Https: //anzctr.org.au/trial/registration/trial review.aspx?id=375347&is clinical trial=false.

Journal: PloS one (2022)
Publication types: Randomized Controlled Trial;Journal Article;Research Support, Non-U.S. Gov't
Novel Allosteric Inhibitor-Derived AKT Proteolysis Targeting Chimeras (PROTACs) Enable Potent and Selective AKT Degradation in KRAS/BRAF Mutant Cells.

Akt is an important target for cancer therapeutics.Significant advancements have been made in developing atp-competitive and allosteric akt inhibitors.Recently, several akt proteolysis targeting chimeras (protacs) derived from atp-competitive akt inhibitors have been reported, including ms21.While ms21 potently degraded akt and inhibited the growth in tumor cells harboring pi3k/pten pathway mutation, it was largely ineffective in degrading akt in kras/braf mutated cells as a single agent.To overcome the akt degradation resistance in kras/braf mutated cells, we developed novel akt protacs derived from an akt allosteric inhibitor, including degrader62(ms15).62displayed potent and selective akt degradation activity and potent antiproliferative activity in kras/braf mutated cancer cells, in addition to pi3k/pten mutated cancer cells.Furthermore,62was bioavailable in mice through intraperitoneal administration.Overall,62is a valuable chemical tool to degrade akt in cells harboring kras/braf mutation and expands the tool box for pharmacologically modulating akt.

Journal: Journal of medicinal chemistry (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't;Research Support, N.I.H., Extramural
Heparan sulfate is necessary for the early formation of nascent fibronectin and collagen I fibrils at matrix assembly sites.

Fibronectin (fn), an essential component of the extracellular matrix (ecm), is assembled via a cell-mediated process in which integrin receptors bind secreted fn and mediate its polymerization into fibrils that extend between cells, ultimately forming an insoluble matrix.Our previous work using mutant chinese hamster ovary (cho) cells identified the glycosaminoglycan heparan sulfate (hs) and its binding to fn as essential for the formation of insoluble fn fibrils.In this study, we investigated the contributions of hs at an early stage of the assembly process using knockdown of exostosin-1 (ext1), one of the glycosyltransferases required for hs chain synthesis.Nih 3t3 fibroblasts with decreased ext1 expression exhibited a significant reduction in both fn and type i collagen in the insoluble matrix.We show that fn fibril formation is initiated at matrix assembly sites, and while these sites were formed by cells with ext1 knockdown, their growth was stunted compared with wild-type cells.The most severe defect observed was in the polymerization of nascent fn fibrils, which was reduced 2.5-fold upon ext1 knockdown.This defect was rescued by the addition of exogenous soluble heparin chains long enough to simultaneously bind multiple fn molecules.The activity of soluble heparin in this process indicates that nascent fibril formation depends on hs more so than on the protein component of a specific hs proteoglycan.Together, our results suggest that heparin or hs is necessary for concentrating and localizing fn molecules at sites of early fibril assembly.

Journal: The Journal of biological chemistry (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural;Research Support, Non-U.S. Gov't
Exposure to the Great Famine in Early Life and the Risk of Obesity in Adulthood: A Report Based on the China Health and Nutrition Survey.

Intrauterine malnutrition has a long-term effect on human health.This study aimed to evaluate the associations between exposure to famine in early life and obesity in adulthood in chinese adults.A total of 5033 participants (22,132 observations) of the china health and nutrition survey (chns) in 1991-2015 were classified into three famine exposure groups according to their birth year: unexposed (1963-1966), fetal-exposed (1959-1962) and childhood-exposed (1955-1958).Compared with the unexposed group, the fetal-exposed group had higher levels of body-mass-index (bmi) and waist-circumference (wc), and higher prevalence of overweight and central obesity, whereas the childhood-exposed group had lower levels of the measurements.However, the positive associations of fetal exposure with bmi, wc and prevalence of overweight and central obesity were attenuated by additionally adjusting for age at survey.Stratified analysis showed that the adverse effect of fetal exposure to famine was only observed in subjects at several specific age-groups, and in men living in rural areas and in women living in more severe famine exposed areas (pfor interaction < 0.05).Our results provide evidence for the weak effect of fetal exposure to famine on body measurements in adulthood, and suggest the importance of severity of famine exposure and timing of exposure.

Journal: Nutrients (2021)
Publication types: Journal Article
IL-17 crosses the blood-brain barrier to trigger neuroinflammation: a novel mechanism in nitroglycerin-induced chronic migraine.

Chronic migraine places a disabling burden on patients, which is extensively modeled by the nitroglycerin (ntg)-treated animal model.Although the nf-κb pathway is involved in an increase in cgrp levels and activation of the trigeminal system in the ntg model, the relationship between ntg and neuroinflammation remains unclear.This study aimed to optimize a chronic ntg rat model with hyperalgesia and the ethological capacity for estimating migraine therapies and to further explore the underlying mechanism of ntg-induced migraine.Methods rats were administered different doses of ntg s.c.Daily or every 2 d; 30 min and 2 h later, the mechanical threshold was tested.After 9 d, the rats were injected with eb or cy5.5 for the permeability assay.The other animals were sacrificed, and then, brainstem and caudal trigeminal ganglion were removed to test cgrp, c-fos and nos activity; cytokines levels in the tissue and serum were measured by elisa; and nf-κb pathway and blood-brain barrier (bbb)-related indicators were analyzed using western blotting.Immunohistochemistry was performed to observe microglial polarization and il-17a+t cell migration in the medulla oblongata.Results ntg (10 mg/kg, s.c., every 2 d for a total of 5 injections) was the optimal condition, resulting in progressive hyperalgesia and migraine behavior.Tnc neuroinflammation with increases in cytokines, cgrp and c-fos and activation of the nf-κb pathway was observed, and these changes were alleviated by ibuprofen.Furthermore, ntg administration increased bbb permeability by altering the levels functional proteins (rage, lrp1, aqp4 and mfsd2a) and structural proteins (zo-1, occludin and ve-cadherin-2) to increase peripheral il-17a permeation into the medulla oblongata, activating microglia and neuroinflammation, and eventually causing hyperalgesia and migraine attack.Conclusions this study confirmed that ntg (10 mg/kg, s.c., every 2 d for a total of 5 injections) was the optimal condition to provoke migraine, resulting in mechanical hyperalgesia and observable migraine-like behavior.Furthermore, il-17a crossed the blood-brain barrier into the medulla oblongata, triggering tnc activation through microglia-mediated neuroinflammation.This process was a novel mechanism in ntg-induced chronic migraine, suggesting that il-17a might be a novel target in the treatment of migraine.

Journal: The journal of headache and pain (2022)
Publication types: Journal Article
Simultaneous resection of coexisting pulmonary and mediastinal lesions by video-assisted thoracic surgery: a case-series study.

With the growing number of patients with coexisting pulmonary and mediastinal lesions detected, reports about simultaneous video-assisted thoracic surgery (vats) for these concurrent diseases are still rare.To further explore the safety and effectiveness of simultaneous resection of pulmonary and mediastinal lesions by uniportal or biportal vats, we retrospectively analyzed the clinical data of the largest series of cases to date.Methods from july 2018 to july 2021, all patients whose pulmonary lesions and mediastinal tumors were resected simultaneously in our institution were retrospectively reviewed.Their demographic and clinical data were collected and analyzed.Results a total of 54 patients were enrolled, of whom 44 underwent unilateral uniportal vats, 3 underwent bilateral uniportal vats and 7 underwent unilateral biportal vats.Seven cases were converted to thoracotomy during surgery.For the remaining 47 patients with various demographic and clinical characteristics, most of the operations were completed within 3 h (n = 33, 70.2%) with blood loss of no more than 100 m l (n = 43, 91.5%).The duration of chest tube drainage was 5.66 ± 3.34 days, and the average daily volume was 196.90 ± 122.31 m l.Four cases of postoperative complications occurred during hospitalization.The length of postoperative hospital stay was 8.60 ± 3.63 days.No severe complications or deaths were observed during follow-up.Conclusions uniportal and biportal vats are safe and effective for simultaneous resection of selected coexisting pulmonary and mediastinal lesions, but the indications and operational details need more evaluation.

Journal: BMC surgery (2022)
Publication types: Journal Article
Examination of applicability of the ISO slab phantom as calibration phantom for the new ICRU 95 operational quantity personal dose.

In this article, the proposal of the international commission on radiation units and measurements/international commission on radiological protection (icru/icrp), that the iso slab phantom should continue to be used as a calibration phantom for the new icru report 95 operational quantity personal dose should be legitimised by simulation and performance of experiments to determine backscatter factors on the iso slab phantom and, in comparison, on an anthropomorphic alderson rando phantom.The scope of this work was restricted to the photon energy range of radiation qualities commonly used in x-ray diagnostics.For this purpose, a shadow-free diagnostic (sfd) ionisation chamber was used to measure backscatter factors for x radiation in the energy range of 24 ke v to 118 ke v.The monte carlo code mcnp 6.2 was used to validate measurement results on the iso slab phantom.Additionally, the influence of varying the sfd position on the rando phantom on the backscatter factor was determined.Since backscatter factors on the iso slab phantom differ only up to±5% from those on the rando phantom, it could be concluded that it is not necessary to develop a new phantom for calibrations in terms of personal dose.A position variation of the detector by a few centimeters on the surface of the rando phantom causes similarly large deviations and thus alone represents an equally large uncertainty contribution in practical personal dosimetry than that arising from the dissimilarity of the real human body to the iso slab phantom.

Journal: Journal of radiological protection : official journal of the Society for Radiological Protection (2022)
Publication types: Journal Article
Study on the biogas potential of anaerobic digestion of coffee husks wastes in Ethiopia.

The poorly controlled discharge of coffee husks in ethiopia causes severe environmental pollution and is a waste of resources.The volatile solid and carbon content in coffee husks waste indicates that it is rich in organic matter and has huge potential to produce biogas.This study investigated the feasibility of coffee husks to produce biomass through anaerobic digestion, based on temperature, initial p h, inoculum/substrate (i/s) ratio and carbon/nitrogen (c/n) ratio.The study demonstrated that the maximum production of biogas and methane reached 3359.6 ml and 2127.30 ml, respectively, under the conditions of mesophilic temperature (35±1°c), an initial p h of 7, an i/s ratio of 0.75 and a c/n ratio of 30.Based on this result, the effects of trace elements (fe2+, ni2+, co2+) on biogas production and methane content were also explored.Compared with the group with no addition of trace elements, the experiment adding trace elements had significant enhancement effects on the production of biogas and methane, in which fe2+played a leading role (p<0.05).Fe2+promoted the hydrolysis and acidification of coffee husks, resulting in the production of a series of intermediates such as volatile fatty acids and the other kinds of dissolved organic matter.Furthermore, the cooperation of ni2+, co2+and fe2+enhanced the activity of the enzyme system in methanogens, promoting methane production.The results in this paper show that coffee husks have clear biogas potential through anaerobic digestion, and its effective utilization could fulfill the dual purpose of solid waste reclamation and local environmental protection in ethiopia.

Journal: Waste management & research : the journal of the International Solid Wastes and Public Cleansing Association, ISWA (2021)
Publication types: Journal Article
Deubiquitinase Activity Profiling Identifies UCHL1 as a Candidate Oncoprotein That Promotes TGFβ-Induced Breast Cancer Metastasis.

Therapies directed to specific molecular targets are still unmet for patients with triple-negative breast cancer (tnbc).Deubiquitinases (dub) are emerging drug targets.The identification of highly active dubs in tnbc may lead to novel therapies.Experimental design using dub activity probes, we profiled global dub activities in 52 breast cancer cell lines and 52 patients' tumor tissues.To validate our findingsin vivo, we employed both zebrafish and murine breast cancer xenograft models.Cellular and molecular mechanisms were elucidated usingin vivoandin vitrobiochemical methods.A specific inhibitor was synthesized, and its biochemical and biological functions were assessed in a range of assays.Finally, we used patient sera samples to investigate clinical correlations.Results two dub activity profiling approaches identified uchl1 as being highly active in tnbc cell lines and aggressive tumors.Functionally, uchl1 promoted metastasis in zebrafish and murine breast cancer xenograft models.Mechanistically, uchl1 facilitates tgfβ signaling-induced metastasis by protecting tgfβ type i receptor and smad2 from ubiquitination.We found that these responses are potently suppressed by the specific uchl1 inhibitor, 6rk73.Furthermore, uchl1 levels were significantly increased in sera of patients with tnbc, and highly enriched in sera exosomes as well as tnbc cell-conditioned media.Uchl1-enriched exosomes stimulated breast cancer migration and extravasation, suggesting that uchl1 may act in a paracrine manner to promote tumor progression.Conclusions our dub activity profiling identified uchl1 as a candidate oncoprotein that promotes tgfβ-induced breast cancer metastasis and may provide a potential target for tnbc treatment.

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research (2020)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Distal semimembranosus tendinopathy: A narrative review.

Distal semimembranosus tendinopathy is a relatively uncommon diagnosis that can be responsible for medial knee pain.The semimembranosus tendon inserts on the posteromedial knee and is surrounded by the semimembranosus bursa, with both the bursa and tendon potential sources of pain.Similar to other tendinopathies, semimembranosus tendinopathy often occurs with overuse of the musculotendinous unit and is commonly seen in runners.Diagnosis can be made clinically and may be substantiated with use of ultrasound or magnetic resonance imaging.Scant literature exists evaluating the efficacy of treatments for this condition.Consequently, best practice for treatment is inferred from other similar tendinopathies, clinical expertise, and smaller studies on semimembranosus tendinopathy.Extrapolating from other tendinopathies, rehabilitation should be the cornerstone of initial treatment, with focus on kinetic chain and gait abnormalities, hamstring strength and neuromuscular control, and progressive tendon loading.Recalcitrant cases with a coexisting bursopathy can be treated with an ultrasound-guided bursal corticosteroid injection.Future studies may help delineate the optimal treatment regimen for this relatively uncommon diagnosis.

Journal: PM & R : the journal of injury, function, and rehabilitation (2022)
Publication types: Journal Article;Review
Palliative medicine education-Bed Race, The End of Life Board Game in undergraduates.

Educational board games facilitate active learning to conceptualise knowledge, and, promote collaborative learning and team work.Despite increasing interest in them, use in palliative and end of life care has been very limited to date.Method in 'bed race, the end of life game', participants are divided into four teams who move a model hospital bed around a board to collect items (syringe driver; do not attempt resuscitation form; oral hydration gel; a 'heart'; just in case medicines).To obtain items at themed 'checkpoints', each team needs to answer quiz questions, which require application of clinical knowledge and/or communication skills.Pregame and postgame quiz scores and feedback were collected from 12 game sessions involving 251 year 5 medical students.Results 169 (67%) of students completed pregame and postgame anonymous quiz questions and free-text feedback.Postgame quiz scores were higher for each topic, and the difference in the paired pregame and postgame questionnaires was statistically significant (p<0.05).Themes from the free-text feedback included 'engaging and fun'; 'relevant learning'; 'peer learning and team work'.Conclusions educational board games are not a new panacea for education, but the concept can be successfully applied in palliative care.

Journal: BMJ supportive & palliative care (2021)
Publication types: Journal Article
Indicating Care Process Quality: A Multidimensional Scaling Analysis.

And purpose:resident assessments are analyzed by multidimensional scaling.methods:we analyzed observer-based real care and support time in four facilities with 209 residents during two working days; resident, organizational data and pairs of residents were assessed by registered and assistant nurses regarding the dissimilarity of resident pairs.Registered- and assistant nurses dissimilarity assessments are compared to criteria-based nursing management assessment.results:the fits of management criteria matrices as external restrictions are higher in registered nurses' than in assistant nurses' assessments.These differences disappear with low staffing.conclusion:the influence of qualification levels on assessment is affected by staffing.Low complexity of assistant nurses assessments is connected to higher nursing care and support time in groups of demanding residents.

Journal: Journal of nursing measurement (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Effect of melatonin on the clinical outcome of patients with repeated cycles after failed cycles ofin vitrofertilization and intracytoplasmic sperm injection.

To explore whether embryo culture with melatonin (mt) can improve the embryonic development and clinical outcome of patients with repeated cycles afterin vitrofertilization/intracytoplasmic sperm injection (ivf/icsi) failure, immature oocytes from controlled ovarian superovulation cycles were collected forin vitromaturation (ivm) and icsi.The obtained embryos were cultured in 0, 10-11, 10-9, 10-7and 10-5m mt medium respectively, and 10-9m was screened out as the optimal concentration.Subsequently, 140 patients who underwent failed ivf/icsi cycles received 140 cycles of embryo culturein vitrowith a medium containing 10-9m mt, these 140 mt culture cycles were designated as the experimental group (10-9m group), and the control group was the previous failed cycles of patients (0 m group).The results showed that the fertilization, cleavage, high-quality embryo, blastocyst, and high-quality blastocyst rates of the 10-9m group were significantly higher than those of the 0 m group (p< 0.01;p< 0.01;p< 0.0001;p< 0.0001;p< 0.0001).To date, in total, 50 vitrified-warmed cycle transfers have been performed in the 10-9m group and the implantation rate, biochemical pregnancy rate and clinical pregnancy rate were significantly higher than those in the 0 m group (all p< 0.0001).Two healthy infants were delivered successfully and the other 18 women who achieved clinical pregnancy also had good examination indexes.Therefore the application of 10-9m mt to embryo culturesin vitroimproved embryonic development in patients with repeated cycles after failed ivf/icsi cycles and had good clinical outcomes.trial registration: chi ctr2100045552.

Journal: Zygote (Cambridge, England) (2022)
Publication types: Journal Article