syedzayyan/paper_mill_benchmark huggingface dataset:
The rs13347 Polymorphism of the CD44 Gene Is Associated with the Risk of Kidney Stones Disease in the Chinese Han Population of Northeast Sichuan, China

To investigate the association between the rs13347 polymorphism of the cd44 gene and the risk of kidney stone disease (ksd) in the han population of northeast sichuan, china, so as to provide a theoretical basis for the treatment of ksd.Methods.We used pcr-restriction fragment length polymorphism (rflp) technique to perform genotyping at rs13347 locus of the cd44 gene in the ksd group and the gontrol group.Snp hardy-weinberg equilibrium (hwe) testing was used to confirm the balance of genetic inheritance.Multivariate logistic regression analysis was used for the assessment of rs13347 polymorphism and the risk of developing ksd and to compare the relationship between the polymorphism of rs13347 and clinical characteristics of patients with ksd.Results.Genotypic results of rs13347 locus of the cd44 gene in the two groups were consistent with the snp-hwe test, indicating the genetic balance.At the same time, multivariate logistic regression analysis indicated that subjects with ct and tt genotypes at rs13347 in the cd44 gene were more likely to have ksd, and there was a higher prevalence rate in males.Furthermore, carrying allele t at rs13347 was also a risk factor for ksd.In addition, people carrying ct and tt genotypes at rs13347 also have a significantly increased risk of relapsing ksd.Conclusion.The rs13347 polymorphism of the cd44 gene may be associated with the risk of ksd in the han population of northeast sichuan in china, and the recurrence rate of ksd in the carriers of ct and tt genotypes is higher.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
The Role and Mechanism of SIRT6 in Regulating Phenotype Transformation of Vascular Smooth Muscle Cells in Abdominal Aortic Aneurysm

Data mining of current gene expression databases has not been previously performed to determine whether sirtuin 6 (sirt6) expression participates in the pathological process of abdominal aortic aneurysm (aaa).The present study was aimed at investigating the role and mechanism of sirt6 in regulating phenotype transformation of vascular smooth muscle cells (vsmc) in aaa.Methods.Three gene expression microarray datasets of aaa patients in the gene expression omnibus (geo) database and one dataset of sirt6-knockout (ko) mice were selected, and the differentially expressed genes (degs) were identified using geo2r.Furthermore, gene ontology (go) and kyoto encyclopedia of genes and genomes (kegg) analyses of both the aaa-related degs and the sirt6-related degs were conducted.Results.Geo2r analysis showed that the expression of sirt6 was downregulated for three groups and upregulated for one group in the three datasets, and none of them satisfied statistical significance.There were top 5 degs (kynu, nptx2, scrg1, grk5, and rgs5) in both of the human aaa group and sirt6-ko mouse group.Top 25 ontology of the sirt6-ko-related degs showed that several pathways including tryptophan catabolic process to kynurenine and negative regulation of cell growth were enriched in the tissues of thickness aortic wall biopsies of aaa patients.Conclusions.Although sirt6 m rna level itself did not change among aaa patients, sirt6 may play an important role in regulating several signaling pathways with significant association with aaa, suggesting that sirt6 m rna upregulation is a protective factor for vsmc against aaa.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
The Relationship between Expression of Nuclear Factor I and the Progressive Occurrence of Diabetic Retinopathy

The loss of nuclear factor i (nfi) function can lead to defects in muller’s glial differentiation, abnormalities of retinal morphology, and changes in retinal neurons numbers, which are highly involved in diabetic retinopathy (dr).In this study, we addressed the roles of nfia and nfib gene expression in the development of dr by using diabetes mellitus (dm) rat models.Retinal histologies were examined, and the expression of nfia and nfib at m rna and protein levels was detected.The results showed that retinal edema and disordered cell arrangement frequently occurred in dr rats.The expressions of nfia and nfib in retinal tissue were significantly decreased in dm rats with dr complications.After further inhibiting the expression of nfia gene in dm rats by using rna-silencing, majority of dm rats occurred retinopathy and lens fibrosis, which indicated the relationship between decreased expression of nfi and occurrence of retinopathy in dm.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
The Relationship between Angiotensin–Neprilysin Treatment, Echocardiographic Parameters, and NT-proBNP Levels in HFpEF Patients with Acute Decompensated Heart Failure

The valsartan-sacubitril therapy improved the outcomes of patients with acute decompensated heart failure (adhf) of a reduced ejection fraction (hfr ef).In adhf patients with preserved ejection fraction (hfp ef), it is not yet clear whether the same treatment regimen may be safely used to treat adhf.Methods.For this study, hfp ef patients hospitalized due to adhf were enrolled.Following hemodynamic stabilization, patients were randomized into two groups that were treated with enalapril or sacubitril-valsartan.In this trial, the primary efficacy outcomes were changes in echocardiographic parameters and nt-pro bnp levels from baseline to 8 weeks treatment.Results.Arni treatment resulted in a significant decrease in nt-pro bnp levels and an increase in lvef in patients with hfp ef.However, hfp ef patients that underwent arni treatment achieved better outcomes than did patients that underwent acei treatment.Conclusion.Sacubitril-valsartan treatment, which lowered nt-pro bnp levels and improved cardiac function, was more effective in hfp ef patients with acute decompensated heart failure than enalapril.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Clinical Study;
The Potential Mechanism of HDAC1-Catalyzed Histone Crotonylation of Caspase-1 in Nonsmall Cell Lung Cancer

Nonsmall cell lung cancer (nsclc) is a predominant subtype of lung cancer and accounts for over 80% of all lung cancer cases.The resistance to pemetrexed (pem) is frequently occurred and severely affects the nsclc therapy.Proteomic analysis of histones indicated that the histone deacetylase 1 (hdac1) complex could hydrolyze lysine crotonylation on histone3 (h3k18cr), affecting epigenetic regulation in cancers.However, the effect of hdac1-mediated h3k18cr on the pem resistance of nsclc is still unclear.Here, we aimed to explore the function of hdac1-mediated h3k18cr in nsclc pem resistance.The expression of hdac1 was upregulated in clinical nsclc tissues and cell lines and correlated with the poor prognosis of nsclc samples.We constructed the pem-resistant nsclc cell lines, and the depletion of hdac1 remarkably reduced the viability of the cells.The proliferation of pem-resistant nsclc cells was decreased by hdac1 knockdown, and the ic50 of pem was repressed by the silencing of hdac1 in the cells.Mechanically, we identified the enrichment of hdac1 on the promoter of caspase-1 in pem-resistant nsclc cells.The depletion of hdac1 inhibited the enrichment of histone h3k18cr and rna polymerase ii (rna pol ii) on the caspase-1 promoter in the cells.The expression of caspase-1 was suppressed by hdac1 knockdown.The knockdown of hdac1 reduced proliferation of pem-resistant nsclc cells, in which caspase-1 or gsdmd depletion reversed the effect.Clinically, the hdac1 expression was negatively associated with caspase-1 and gsdmd in clinical nsclc tissues, while caspase-1 and gsdmd expression was positively correlated in the samples.Therefore, we concluded that hdac1-catalyzed histone crotonylation of caspase-1 modulates pem sensitivity of nsclc by targeting gsdmd.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;
The Number of Intraoperative Intestinal Venous Circulating Tumor Cells Is a Prognostic Factor for Colorectal Cancer Patients

To assess the association between intestinal venous blood (ivb) circulating tumor cells (ctcs) and clinicopathological parameters in stage i-iii colorectal cancer (crc) patients.Methods.Participants were retrospectively retrieved, who were admitted to our hospital or took annual physical exams between december 1, 2015 and december 31, 2018.A negative enrichment-immunofluorescence in situ hybridization (ne-im fish) technique was used to isolate and identify ctcs.Receiver operating characteristic (roc) curves and youden index values were used to determine the critical ctc cutoff value for the diagnosis of crc.Kaplan-meier and log-rank methods were used to conduct survival analyses, and multivariate cox regression analyses were employed for multivariate corrections to comprehensively evaluate the value of ctcs in the diagnosis of crc.Relationships between ivb ctcs, clinicopathological parameters, and prognosis were then analyzed based upon patient postoperative follow-up data.Results.In total, we retrieved 282 patients including 48 healthy controls, 72 patients with benign colorectal tumors, and 162 crc patients.Crc patients exhibited significantly higher numbers of ctcs relative to control patients or those with benign disease.Ctc numbers in crc patient peripheral blood (pb) and ivb were closely associated with tumor node metastasis (tnm) staging (p &lt; 0.01), carbohydrate antigen-125 (ca-125) levels (p &lt; 0.001), and kras (kirsten rat sarcoma virus oncogene) mutation status (p &lt; 0.001).The disease-free survival (dfs) of patients in the ctc-negative group was significantly longer than that of patients in the ctc-positive group (24.60 ± 13.31 months vs.18.70 ± 10.19 months, p &lt; 0.05), with the same being true with respect to their overall survival (os) (30.60 ± 12.44 months vs.35.25 ± 11.57 months, p &lt; 0.05).A multivariate analysis revealed that the detection ≥2 ctcs/3.2 ml was independently associated with poorer dfs and os.Ctc counts were independently predictive of crc patients tnm staging, ca-125, and kras mutation status in both univariate and multivariate cox proportional hazards regression analyses.Conclusion.Ctcs are valuable biomarkers that can be monitored to predict crc patient disease progression.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;
The Low-Carbon Effect Measurement of the Structural Adjustment of the Ice and Snow Industry Based on Artificial Intelligence

Ice and snow economy is an economy characterized by ice and snow, and its foundation is ice and snow resources.The ice and snow economy covers the tertiary industry of ice and snow activities, and its core power comes from ice and snow tourism, which promotes the common development of manufacturing, transportation, catering, retail, and other industries.This paper studies the low-carbon effect measurement analysis of the structural adjustment of the ice and snow industry based on artificial intelligence, which proves that the economic benefits and carbon emissions of the structural adjustment of the ice and snow industry will be greatly improved after the addition of artificial intelligence technology.For this research, a series of investigation experiments are conducted, and the ice and snow industry in heilongjiang province is selected for analysis.First, the important position of artificial intelligence technology in today’s information society and its practicality are fully analyzed, and then its feasibility in combination with the structural adjustment of the ice and snow industry is analyzed.Second, it analyzes the characteristics of the structural adjustment of the ice and snow industry while taking into account its requirements for measuring low-carbon effects.The mathematical model of industrial structure optimization based on low-carbon constraints is adopted in the application model of artificial intelligence technology in the adjustment of the ice and snow industry structure, and the effects of carbon emissions before and after structural adjustments in the ice and snow industry are calculated to achieve a better industrial structure adjustment plan.Experimental data shows that with the help of artificial intelligence in the process of structural adjustment of the ice and snow industry, the average income of residents has increased by nearly 7%, and the transformation of the ice and snow industry’s contribution to the reduction of carbon emissions has rapidly increased to 12.24%.

Journal: Security and Communication Networks (2022)
Publication types: Research Article;
The Immune Infiltration in HNSCC and Its Clinical Value: A Comprehensive Study Based on the TCGA and GEO Databases

Being potential field of research for tumor immunological therapy, the head and neck squamous cell carcinoma (hnscc) is one of most discussed types of tumor.Recently, some clinical trials have also used immunological therapy and demonstrated a subset of hnscc patients who have shown a clear longer survival time.Objective.To conduct further studies and deeper research in the immunological oncology of hnscc, a more detailed description and comprehending of the complicated landscape of immune infiltrative may be required.Methods.Firstly, we have described the fraction of different infiltrating immune cells in the hnscc tumor and then compared it to the normal tissue, and secondly, we have explored the clinical implications of various infiltrated immune cell fractions meticulously.The gene expression profiles of hnscc tissue were obtained from databases of tcga and geo and utilized the deconvolution algorithm (cibersort) to presume the fractions of 22 several immune sensitive cells.Results.Our results indicated that the immune infiltrating cell fractions were considerably different between hnscc tumor tissue and paired normal tissue, but at the same time, we found a potential internal correlation among the immune cells and also showed the association between immune infiltrating cells and their clinical characteristics.It is worth noting that the resting dendritic cells and m1 macrophages were linked with a favorable prognosis, while the cd4+ t cells with a poorer outcome.Conclusion.Fractions of immune cell percentage were also associated with tumors’ pathological grade, age, and tnm stage.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Research Article;
The Imbalance Expression of DLX3 May Perform Critical Function in the Occurrence and Progression of Preeclampsia

The present research focuses on preeclampsia (pe), a clinically relevant pregnancy disease.To date, the majority of research on pe was centered on placental insufficiency.However, the genes that regulate these processes, and the exact molecular mechanisms modulating these processes, are still unclear.Methods.We obtained placentae from a clinically well-specified group of patients with preeclampsia and gestationally matched control pregnancies in order to evaluate the expression of homeobox gene dlx3 by immunohistochemical staining, real-time pcr, and western immunoblotting and determine the function of dlx3 utilizing lentivirus transfection in htr-8/svneo cells.Results.In the present study, we detected dlx3 expression in a clinically well defined cohort of preeclampsia-affected and gestation-matched control pregnancies.As opposed to the controls, dlx3 was overexpressed in preeclampsia-affected placentae.Moreover, we found that the in vitro cell growth and invasive ability of htr8/svneo cells was enhanced by the exogenous overexpression of dlx3 ( p &lt; 0.05 ).It can be seen that dlx3 influences the cell cycle of htr-8/svneo cells in vitro.Conclusions.Dlx3 has been shown to be strongly related to normal placental growth as well as the pathophysiology of preeclampsia.The imbalanced expression of dlx3 may perform an integral function in the occurrence and progression of preeclampsia.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
The Efficacy of Needle-Warming Moxibustion Combined with Hyperbaric Oxygen Therapy for Ischemic Stroke and Its Effect on Neurological Function

To elucidate the therapeutic efficacy of needle-warming moxibustion (nwm) combined with hyperbaric oxygen therapy (hbot) in the treatment of patients with ischemic stroke and its effect on neurological function.Methods.One hundred patients with ischemic stroke admitted to the xuzhou medical university affiliated hospital of lianyungang from january 2019 to july 2021 were enrolled.Among them, 45 patients treated with nwm were set as the control group, and the rest 55 patients treated by nwm combined with hbot were included in the research group.The curative effect, neurological deficit score, activity of daily living (adl), balance ability, and the levels of serum proinflammatory factors in both groups were observed and recorded.Of them, the neurological deficit of patients was evaluated by the national institutes of health stroke scale (nhiss), the adl ability was determined by the barthel index score, and the balance ability was assessed by the berg balance scale.Results.The total effective rate of the research group was higher than that of the control group.Better adl and balance ability and milder neurologic impairment were determined in the research group compared with the control group.After treatment, the secretion levels of proinflammatory factors such as c-reactive protein (crp), tumor necrosis factor-α (tnf-α), and interleukin-8 (il-8) in the serum of patients in the research group were statistically lower than those before treatment and the control group.Conclusions.Nwm combined with hbot is effective in the treatment of patients with ischemic stroke, which can not only improve patients’ neurological function, adl, and balance ability but also inhibit serum inflammatory reactions.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Clinical Study;
The Effect of Symptom Clusters and Sleep Disorder on Quality of Life among Patients with Chronic Obstructive Pulmonary Disease

Symptoms (cough, dyspnea, fatigue, depression, and sleep disorder) in chronic obstructive pulmonary disease (copd) are related to poor quality of life (qol).Better understanding of the symptom clusters (scs) and sleep disorder in copd patients could help to accelerate the development of symptom-management interventions.Objective.We aim to explore the effect of sleep disorder and symptom clusters on the qol in patients with copd.Methods.223 patients with stable copd from november 2019 to november 2020 at the affiliated people’s hospital of ningbo university in china were included in this cross-sectional survey.A demographic and clinical characteristics questionnaire, the revised memorial symptom assessment scale (rmsas), the pittsburgh sleep quality index (psqi), and the st george respiratory questionnaire for copd (sgrq-c) were completed by the patients.Exploratory factor analysis was conducted to extract scs, and logistic regression analysis was performed to analyze the risk factors affecting qol.Results.Three clusters were extracted: respiratory functional cluster, emotional cluster, and fatigue-sleep cluster.70.4% of the participants were poor sleepers.Subgroup analysis showed that copd patients with poor sleep quality were significantly different in qol, emotional cluster, and fatigue-sleep cluster compared with patients with normal sleep.In multiple regression, sleep quality and respiratory functional cluster were associated with qol.Conclusion.In patients with stable copd, three symptom clusters were explored.Symptom clusters correlate with clinical features and negatively affect qol.Appropriate interventions are expected to inform future approaches to symptom management.Future studies are needed to test interventions that may be effective at improving the qol of copd patients.

Journal: Journal of Healthcare Engineering (2021)
Publication types: Research Article;
The Effect of Hyperlipidemia on Peri-implant Health: A Clinical and Radiographical Prospective Study

High levels of cholesterol and triglycerides may have a negative effect on the immune system and bone health, leading to lower bone mineral density, an increased risk of osteoporosis, and bone fractures, and could therefore also be related to a significant worsening of peri‐implant health.The purpose of the following study was to evaluate whether the altered lipid profile in patients who undergo implant insertion surgery represents a prognostic factor capable of influencing clinical outcomes.This prospective observational study was conducted on 93 subjects; patients were required to have taken blood tests to obtain triglycerides (tg), total cholesterol, low‐density lipoprotein (ldl), and high‐density lipoprotein (hdl) levels prior to the surgical procedure to classify them according to current american heart association guidelines.The outcomes considered were marginal bone loss (mbl) 3 years after implant placement, full‐mouth plaque score (fmps), and full‐mouth bleeding score (fmbs) 3 years after surgery.A statistically significant correlation was found between hypertriglyceridemia and mbl as well as between total cholesterol and mbl.There is no statistically significant correlation between the variables analyzed and the secondary outcomes 3 years after implant placement.Peri‐implant marginal bone loss may be influenced by hyperlipidemia.However, further studies are needed, with larger samples and more extensive follow‐ups, to confirm these results.

Journal: BioMed Research International (2023)
Publication types: Research Article;
The Diagnostic Efficacy of the American College of Radiology (ACR) Thyroid Imaging Report and Data System (TI-RADS) and the American Thyroid Association (ATA) Risk Stratification Systems for Thyroid Nodules

This study is aimed at evaluating the diagnostic efficacy of ultrasound-based risk stratification for thyroid nodules in the american college of radiology (acr) thyroid imaging reporting and data system (ti-rads) and the american thyroid association (ata) risk stratification systems.Methods.286 patients with thyroid cancer were included in the tumor group, with 259 nontumor cases included in the nontumor group.The acr ti-rads and ata risk stratification systems assessed all thyroid nodules for malignant risks.The diagnostic effect of acr and ata risk stratification system for thyroid nodules was evaluated by receiver operating characteristic (roc) analysis using postoperative pathological diagnosis as the gold standard.Results.The distributions and mean scores of acr and ata rating risk stratification were significantly different between the tumor and nontumor groups.The lesion diameter &gt; 1 cm subgroup had higher malignant ultrasound feature rates detected and acr and ata scores.A significant difference was not found in the acr and ata scores between patients with or without hashimoto’s disease.The area under the receiver operating curve (auc) for the acr ti-rads and the ata systems was 0.891 and 0.896, respectively.The acr had better specificity (0.90) while the ata system had higher sensitivity (0.92), with both scenarios having almost the same overall diagnostic accuracy (0.84).Conclusion.Both the acr ti-rads and the ata risk stratification systems provide a clinically feasible thyroid malignant risk classification, with high thyroid nodule malignant risk diagnostic efficacy.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;