syedzayyan/paper_mill_benchmark huggingface dataset:
Long Intergenic Nonprotein Coding RNA 00174 Aggravates Lung Squamous Cell Carcinoma Progression via MicroRNA-185-5p/Nuclear Factor IX axis

Extensive studies have presented that long noncoding rnas (lnc rnas) are closely implicated in the pathogenesis of various human malignancies, including lung squamous cell carcinoma (lusc).This study explored the biological role and the underlying mechanism of long intergenic nonprotein coding rna 00174 (linc00174) in lusc.Linc00174 expression was measured by reverse transcription quantitative real‐time polymerase chain reaction (rt‐q pcr).Both in vitro and in vivo experiments were conducted to determine linc00174 function in lusc.Mechanical assays were performed to investigate the molecular mechanism involving linc00174 and related genes.Linc00174 expression was high in lusc cells.Silencing of linc00174 could restrain lusc cells proliferation, migration, and invasion while promoting cell apoptosis.Mechanically, linc00174 could interact with mi r‐185‐5p to upregulate nuclear factor ix (nfix), which was the direct target gene of mi r‐185‐5p.Notably, nfix elevation could rescue the repressing effect of linc00174 silence on lusc cell malignant behaviors.Our data suggested that linc00174 aggravated lusc progression via serving as a competing endogenous rna (ce rna) to sponge mi r‐185‐5p and ultimately upregulate nfix, which offered a promising novel target for lusc therapy.

Journal: BioMed Research International (2022)
Publication types: Research Article;
lncRNA Vgll3 Regulates the Activated Proliferation of Mouse Myocardial Fibroblasts through TGF-β3-Related Pathway

Cardiac fibrosis is a risk factor leading to various cardiac diseases, and its mechanism has not been clarified.However, long noncoding rna (lnc rna) can mediate the pathological process of cardiac fibrosis.Objective .This study is aimed at determining the pathological role of lnc rna vgll3 in cardiac fibrosis and explore its potential mechanism.Methods .Myocardium fibroblasts (cfs) were isolated from mice and stimulated with angiotensin ii (ang‐ii).The expression of vgll3 and transforming growth factor‐ β 3 (tgf‐ β 3) were detected by real‐time fluorescence quantitative pcr (q pcr).Double luciferase reporter gene and western blot analysis (wb) were used to detect the effect of vgll3 on tgf‐ β 3 expression.The q pcr and wb were used to detect tgf‐ β 3 pathway markers such as tgf‐ β 3 and smad4, as well as cardiac fibrosis markers such as α ‐smooth muscle actin ( α ‐sma), fibronectin (fn), and type i collagen (col1).The proliferation of cfs in mice was analyzed by cell counting kit‐8 (cck8) and 5‐bromo‐2‐deoxyuracil (ed u) method.Results .Upregulation of vgll3 promoted the expression of tgf‐ β 3 and its downstream molecules in mouse cfs, while silencing of vgll3 inhibited the tgf‐ β 3 pathway.Upregulation of vgll3 significantly promoted the activation and proliferation of mouse cfs cells.It promoted the m rna and protein levels of α ‐sma, fn, col1, and col3, while silencing the expression of vgll3 had the opposite effect.The above effects of upregulation of vgll3 were counteracted by tgf‐ β 3 knockdown intervention.Conclusion .Vgll3 can promote the activation and proliferation of cfs in mice by activating tgf‐ β 3‐related pathway.

Journal: BioMed Research International (2022)
Publication types: Research Article;
LINC00092 Modulates Oxidative Stress and Glycolysis of Breast Cancer Cells via Pyruvate Carboxylase-Mediated AKT/mTOR Pathway

The discovery of noncoding rnas (nc rnas) offers new options for cancer-targeted therapy.This study is aimed at exploring the regulatory function of linc00092 on breast cancer (bc) oxidative stress and glycolysis, along with internal mechanism concerning pyruvate carboxylase (pc).Methods.Bioinformatics analysis was used to explore linc00092 (or friend leukemia virus integration 1 (fli1)) expression on bc progression, as well as oxidative stress and glycolysis in bc.After linc00092 overexpression or silence, bc cell viability, proliferation, migration, invasion, oxidative stress, glycolysis, and akt/m tor pathway were detected.Following 2-dg, sc79, or mk2206 treatment, effects of linc00092 on bc cells were measured.Moreover, regulatory activity of linc00092 in pc expression was analyzed.Whether pc participated in the modulation of linc00092 on bc cell functions was explored.Results.Linc00092 was lowly expressed in bc and negatively related to bc progression.Fli1 bound to linc00092 promoter to positively modulate linc00092.Linc00092 overexpression inhibited bc cell proliferation, migration, invasion, oxidative stress, glycolysis, and akt/m tor pathway and likewise suppressed bc growth in vivo.Silence of linc00092 had opposite influences.2-dg partially reversed the linc00092 silence-resulted increase of bc cell proliferation.Sc79 alleviated the function of linc00092 overexpression on bc cell functions.Mk2206 had the contrary influence of sc79.Besides, linc00092 bound to pc to modulate ubiquitination degradation of pc protein.Pc took part in the influences of linc00092 on bc cell functions.Conclusions.Linc0092 modulates oxidative stress and glycolysis of bc cells via the pc-mediated akt/m tor pathway, which is possibly a target for bc diagnosis and therapy.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Levels and Significance of Tumor Markers and Cytokines in Serum and Peritoneal Lavage Fluid of Patients with Peritoneal Metastasis of Gastric Cancer

The paper is written to investigate the levels and significance of tumor markers [carcinoembryonic antigen (cea), carbohydrate antigen 125 (ca125), and carbohydrate antigen 19‐9 (ca19‐9)] and cytokines [interleukin‐6 (il‐6), il‐4, and il‐2] in serum and peritoneal lavage fluid of patients with peritoneal metastasis of gastric cancer.For this research, 145 patients with gastric cancer treated in our hospital were divided into peritoneal metastasis group ( n = 25), other metastasis group ( n = 32), and nonmetastasis group ( n = 88) according to the occurrence of metastasis.At the same time, the levels of serum tumor markers and cytokines and tumor markers and cytokines in intraoperative peritoneal lavage fluid were compared among the three groups.The results showed that the proportion of tnm stage iii in peritoneal metastasis group and other metastasis group was 68.00% and 62.50%, respectively, and the proportion of tumor &gt;5 cm was 64.00% and 59.38%, respectively, which was significantly higher than that in the control group.The 1‐year survival rate of peritoneal metastasis group and other metastasis group was 44.00% and 40.63%, respectively, which was significantly lower than that of nonmetastasis group ( p &lt; 0.05).the serum levels of cea, ca125, ca19‐9, il‐6, il‐4, and il‐2 in peritoneal metastasis group and other metastasis group were higher than those in nonmetastasis group.The intraoperative peritoneal lavage fluid cea, ca125, and il‐6 were 13.41 ± 3.72 ng/ml, 8.97 ± 1.33 u/ml, and 1.85 ± 0.44 pg/ml, respectively, which were higher than those in other metastasis groups and nonmetastasis groups ( p &lt; 0.05).There was no significant difference in the levels of ca19‐9, il‐4, and il‐2 in peritoneal lavage fluid among peritoneal metastasis group, other metastasis groups, and nonmetastasis groups ( p &gt; 0.05); the areas under the roc curve of intraoperative peritoneal lavage fluid cea, ca125, and il‐6 in predicting peritoneal metastasis were 0.850, 0.902, and 0.806, respectively, p &lt; 0.05.Thus, the conclusion is that peritoneal lavage fluid cea, ca125, and il‐6 have certain application value in predicting and diagnosing peritoneal metastasis of gastric cancer, while the other indexes have no application value.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Level Set Image Feature Detection and Application in COVID-19 Image Feature Knowledge Detection

Artificial intelligence (ai) scholars and mediciners have reported ai systems that accurately detect medical imaging and covid‐19 in chest images.However, the robustness of these models remains unclear for the segmentation of images with nonuniform density distribution or the multiphase target.The most representative one is the chan‐vese (cv) image segmentation model.In this paper, we demonstrate that the recent level set (lv) model has excellent performance on the detection of target characteristics from medical imaging relying on the filtering variational method based on the global medical pathology facture.We observe that the capability of the filtering variational method to obtain image feature quality is better than other lv models.This research reveals a far‐reaching problem in medical‐imaging ai knowledge detection.In addition, from the analysis of experimental results, the algorithm proposed in this paper has a good effect on detecting the lung region feature information of covid‐19 images and also proves that the algorithm has good adaptability in processing different images.These findings demonstrate that the proposed lv method should be seen as an effective clinically adjunctive method using machine‐learning healthcare models.

Journal: BioMed Research International (2023)
Publication types: Research Article;
Lead Exposure Induced Neural Stem Cells Death via Notch Signaling Pathway and Gut-Brain Axis

Numerous studies have examined the effects of lead (pb) on cognitive ability.It is essential for the brain to maintain its functions through the differentiation of neural stem cells into various types of cells.Despite this, it remains unclear how pb exposure affects neural stem cells and how it does, so the pb-exposed mice were treated with the notch inhibitor dapt after we established the pb exposure models.Neuronal stem cells and autophagy were assessed by immunofluorescence staining and western blot.The microbiota of the feces was also analyzed using the 16s r rna amplicon sequencing technique.In this study, we found that pb exposure caused neural injuries and deficits in neural stem cells, whereas dapt rescued the damage.With dapt, pb-induced autophagy was partially reversed.Exposure to pb also reduced inflammation and damaged gut barrier function.Furthermore, pb exposure led to low bacterial diversity, an increase in pathogen abundance, and an unusual mode of interaction.Taken together, this study revealed that damages in neural stem cells contributed largely to cognitive impairment during pb exposure, and this process was partially dependent on the notch pathway and gut dysbiosis.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Large-Area Saline-Alkali-Tolerant Rice Growth Environment Monitoring System Based on LoRa+UAV

A wireless sensor network (wsn) monitoring system for saline-alkali-tolerant rice based on long-range + unmanned aerial vehicle (lo ra + uav) has been proposed in this paper to meet the requirements of difficult deployment, low scalability, and poor network reliability of traditional farmland environmental monitoring systems.The system automatically forms a double-layer star topology network through the terminal node and central node (intranet), central node, and uav mobile node (extranet).Data transmission is carried out through lo ra technology, routing planning is carried out for uav mobile receiving nodes at different flight altitudes, and an early warning mechanism for uav energy is added.It effectively prevents the uav from destroying the communication capability of the external network due to energy exhaustion during operation.The system transmits information remotely to the server of the management center through a 5g network, which analyzes and processes the data and publishes it on the web.The system has been tested in the field, and the reliable communication range of the node is 1∼4 km under the saline-alkali-tolerant rice environment, and the service life of the terminal node can reach more than 3 months.During continuous operation, the maximum packet loss rate of the internal and external integrated networks is 1.45%.The system can stably and real-time monitor saline-alkali-tolerant rice environmental information, and the error rate is less than 10%, which can meet the performance requirements of the saline-alkali-tolerant rice environmental monitoring systems, such as low power consumption, wide coverage, stability, and reliability, and has a good engineering significance.

Journal: Advances in Materials Science and Engineering (2022)
Publication types: Research Article;
Long noncoding RNA CASC2 inhibits ox-LDL-mediated vascular smooth muscle cells proliferation and migration via the regulation of miR-532-3p/PAPD5

Background studies have demonstrated that long noncoding rnas (lnc rnas) have essential impacts on the development of atherosclerosis (as).This study aimed to identify the role and functional mechanism of lnc rna casc2 in the development and migration of vascular smooth muscle cells (vsmcs).Method the serum of 40 pairs of as patients and healthy volunteers were collected and the expression of casc2 was evaluated.Q rt-pcr and western blotting were carried out to examine the expression levels of at m rna and protein level, repectively.Cell proliferation assay, colony formation assay, transwell migration assay, dual-luciferase reporter assay, and wound healing assay were conducted to evaluate cell proliferation, colony formation, migration, transcription, targeting, and self-restoration.Results the expression levels of casc2 were decreased, while the expression levels of mi r-532-3p were elevated in as patient samples and vsmcs.Overexpression of casc2 inhibited the proliferation and migration of vsmcs and enhanced cell apoptosis.Casc2 inhibited the expression of mi r-532-3p, and inversely upregulated the expression of papd5, which was a target of mi r-532-3p.In addition, knockdown of mi r-532-3p-mimic and papd5 could attenuate the impact of overexpression of casc2 on proliferation, migration, and apoptosis in ox-ldl-vsmcs.Conclusion casc2 suppressed cell reproduction and promoted cell apoptosis by regulating the mi r-532-3p/papd5 axis in ox-ldl-mediated vsmcs.This might be important for as therapeutics.

Journal: Molecular Medicine (2020)
Publication types: Research Article;
Investigating Celastrol’s Anti-DCM Targets and Mechanisms via Network Pharmacology and Experimental Validation

And purpose .Dcm (diabetic cardiomyopathy), which may lead to significant complications including cardiovascular lesions, arrhythmia, and even heart failure, has a beginning element now known to be myocardial energy rebuilding.There are limited research on celastrol’s ability to guard against this in the united states and elsewhere.Since it has not been known, whether celastrol could reverse the early energy remodeling process, thus, it was hypothesized that triptolide celastrol is suitable for the reversal of early myocardial energy remodeling in dcm.And our aim is to predict the targets and underlying mechanism of celastrol in reversing the early energy remodeling for dcm.Methods .Data from tcmsp and geo databases were utilized to identify targets for celastrol on dcm.The relationship between the major targets and conventional glycolipid metabolism was obtained with spearman correlation analysis.Experiments on animals were conducted utilizing healthy control (hc), low‐dose celastrol interventions (cl), and no intervention groups (nc), all of which had 8 sd rats in each group.To study alterations in signaling molecules, rt‐pcr was performed.Results .There were 76 common targets and 5 major targets for celastrol‐dcm.Celastrol have been found to regulate age‐rage, tnf, mapk, toll‐like receptors, insulin resistance, and other signaling pathways, and they are closely linked to adipocytokines, fatty acid metabolism, glycolipid biosynthesis, and glycosylphosphati‐dylinositol biosynthesis on dcm.These five major targets have been found to regulate these pathways.Experiments on rats indicated that p38 mapk was considerably elevated in the cardiac tissue from rats in the cl and nc groups compared to the hc group, and the difference was statistically significant ( p &lt; 0.01).Significant differences were seen between the cl and nc groups in p38 mapk levels, with a statistical significance level of less than 0.05.Conclusion .Celastrol may play a role in reversing energy remodeling, anti‐inflammation, and oxidative stress via modulating p38 protein expression in the mapk pathway, which have been shown in the treatment of dcm.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Intestinal Flora Balance Therapy Based on Probiotic Support Improves Cognitive Function and Symptoms in Patients with Alzheimer’s Disease: A Systematic Review and Meta-analysis

The clinical value of intestinal flora balance therapy based on probiotic support in improving cognitive function and symptoms of patients with alzheimer’s disease was to systematically evaluate, so as to provide evidence‐based medicine basis for the promotion and use of this therapy.Methods .The randomized controlled trials (rcts) were searched for the improvement of cognitive function and symptoms of patients with alzheimer’s disease by intestinal flora balance therapy supported mainly by probiotics in pub med, embase, science direct, cochrane library, china knowledge network database (cnki), china vip database, wanfang database, and china biomedical literature database (cbm) online database (rct).Data were extracted independently by two researchers, and the literature was assessed for risk of bias according to the cochrane handbook 5.1.0 criteria.The data were meta‐analyzed using rev man 5.4 statistical software.Results .Finally, 5 randomized controlled trials were included, with a total sample size of 386 cases.The results of meta‐analysis showed that chi 2 = 13.14, df = 2, p = 0.001, and i 2 = 85 % showed significant heterogeneity in the inclusion of the study data.Probiotic‐supported intestinal microflora balance therapy improves cognitive function in patients with alzheimer’s disease.Through meta‐analysis of transient memory scores, it is concluded that intestinal flora balance therapy based on probiotic support can improve transient memory in patients with alzheimer’s disease.Meta‐analysis of adas‐cog score showed that intestinal flora balance therapy supported by probiotics could improve the cognitive function of patients with alzheimer’s disease.The adl score was analyzed by meta, and the heterogeneity test result was chi 2 = 0.79, df = 1, p = 0.37 &gt; 0.05, and i 2 = 0 % , indicating that the intestinal flora balance therapy supported by probiotics can improve the ability of daily living of patients with alzheimer’s disease.Conclusion .Intestinal flora balance therapy based on probiotic support can effectively improve cognitive function, instantaneous memory, and ability of daily life in patients with alzheimer’s disease.However, more studies and long‐term follow‐up studies with higher methodological quality are needed to further verify.

Journal: BioMed Research International (2022)
Publication types: Meta-Analysis;Review Article;
Intelligent Recommendation System of Dance Art Video Resources Based on the Wireless Network

Ing at the problems of low recommendation accuracy and long recommendation time of the traditional dance art video resource intelligent recommendation system, an intelligent recommendation system of dance art video resource based on the wireless network was designed.In this paper, the hardware part of the smart recommendation system of dance art video resources is designed with the mcu as the system control core, the max3232 chip as the hardware transceiver chip, and the rs323 bus circuit.On this basis, according to the user’s existing relationship, a social network is constructed through wireless network technology to obtain user information, bayesian network is used to predict the user’s preference for dance videos.The probability that users like dance videos is given in the form of data, and related videos of dance art are recommended based on intelligent video resources.The simulation results show that the designed system has higher accuracy and shorter recommendation time for dance art video resources.

Journal: Security and Communication Networks (2021)
Publication types: Research Article;
Inflammation and Oxidative Stress Role of S100A12 as a Potential Diagnostic and Therapeutic Biomarker in Acute Myocardial Infarction

Acute myocardial infarction (ami) is one of the most serious cardiovascular diseases with high morbidity and mortality.Numerous studies have indicated that s100a12 may has an essential role in the occurrence and development of ami, and in-depth studies are currently lacking.The purpose of this study is to investigate the effect of s100a12 on inflammation and oxidative stress and to determine its clinical applicability in ami.Here, ami datasets used to explore the expression pattern of s100a12 in ami were derived from the gene expression omnibus (geo) database.The pooled standard average deviation (smd) was calculated to further determine s100a12 expression.The overlapping differentially expressed genes (degs) contained in all included datasets were recognized by the geo2r tool.Then, functional enrichment analyses, including gene ontology (go) and kyoto encyclopedia of genes and genomes (kegg) analyses, were carried out to determine the molecular function of overlapping degs.Gene set enrichment analysis (gsea) was conducted to determine unrevealed mechanisms of s100a12.Summary receiver operating characteristic (sroc) curve analysis and receiver operating characteristic (roc) curve analysis were carried out to identify the diagnostic capabilities of s100a12.Moreover, we screened mi rnas targeting s100a12 using three online databases (mi rwalk, target scan, and mi rdb).In addition, by comprehensively using enzyme-linked immunosorbent assay (elisa), real-time quantitative pcr (rt–q pcr), western blotting (wb) methods, etc., we used the ac16 cells to validate the expression and underlying mechanism of s100a12.In our study, five datasets related to ami, gse24519, gse60993, gse66360, gse97320, and gse48060 were included; 412 overlapping degs were identified.Protein-protein interaction (ppi) network and functional analyses showed that s100a12 was a pivotal gene related to inflammation and oxidative stress.Then, s100a12 overexpression was identified based on the included datasets.The pooled standard average deviation (smd) also showed that s100a12 was upregulated in ami ( smd = 1.36 , 95% ci: 0.70-2.03, p = 0.024 ).The sroc curve analysis result suggested that s100a12 had remarkable diagnostic ability in ami ( auc = 0.90 , 95% ci: 0.87-0.92).And nine mi rnas targeting s100a12 were also identified.Additionally, the overexpression of s100a12 was further confirmed that it maybe promote inflammation and oxidative stress in ami through comprehensive in vitro experiments.In summary, our study suggests that overexpressed s100a12 may be a latent diagnostic biomarker and therapeutic target of ami that induces excessive inflammation and oxidative stress.Nine mi rnas targeting s100a12 may play a crucial role in ami, but further studies are still needed.Our work provides a positive inspiration for the in-depth study of s100a12 in ami.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;