syedzayyan/paper_mill_benchmark huggingface dataset:
Biofunctionalities of unprocessed and processed flours of Australian lupin cultivars: Antidiabetic and organ protective potential studies.

As lupin has emerged popularity as dietary protein and nutritional source, our present research was aimed to demonstrate the antidiabetic and organ-protective activities of nine cultivars of australian sweet lupin seed flours by means of in vitro and in vivo assays accompanied by identification of their bioactive phytocompounds and exploration of underlying mechanisms of their hypoglycemic activity using in silico approach.In vitro α-amylase and α-glucosidase activities inhibition and glucose uptake assays identified jenabillup seed flours for exhibiting the most potential antidiabetic activity amongst the nine cultivars.In vivo antidiabetic and major organ-protective activities were investigated on streptozotocin-induced hyperglycemia and organ damages in wister rat model.Along with attenuating hyperglycemia and retreating major organ damages, the biochemical imbalance in cardiac, hepatic and renal markers were well-balanced by jenabillup seed flours treatment.These activities of lupin seed flours were insignificantly affected by thermal processing.Moreover, in silico investigation of 106 phytochemicals identified by gas chromatography-mass spectroscopy (gc-ms) analysis of the seed flour extracts of nine cultivars revealed that more than 35% of compounds possess moderate to high binding affinity to α-amylase and α-glucosidase enzymes.These bioactive compounds act synergistically to exert potential hypoglycemic activity.Cross-docking and binding energy calculation by molecular mechanics/generalized born volume integration (mm/gbvi) model suggest actinomycin c2 as a potential inhibitor of both α-amylase and α-glucosidase enzymes.These findings acclaim that australian sweet lupin seed flours may be considered not only as functional food, but also for further development of effective drugs in pharmaceuticals in the treatment of diabetes mellitus and resultant organ damages.

Journal: Food research international (Ottawa, Ont.) (2021)
Publication types: Journal Article
Investigation of Fire Effects on Reinforced Concrete Members via Finite Element Analysis.

Structural deterioration during fire leads to significant economic losses, severe injuries, and deaths.Research to accurately estimate the impact of fire on structural security and performance, and to identify ways to reduce it, has been increasing recently with capital investments in the building and infrastructure sectors.This research aims to establish a reliable algorithm for simulating the behavior of reinforced concrete (rc) beams under thermal and structural loads.The proposed algorithm is based on the combination of thermal and structural analyses using the sequential link technique.These analyses use material characteristics such as conductivity, specific heat, stress-strain relationship, and thermal expansion to capture thermal and structural responses during the heating phases according to eurocode 1 and eurocode 2 using the finite element method.Beam models in the study, which have been exposed to the iso-834 fire curve, were designed to exhibit flexural failure.Nonlinear numerical analysis results have mostly coincided with the previous studies regarding the residual load-bearing capacity.Depending on the outcomes of the previous experimental studies, an rc member's structural strength increases when the internal temperature is between 150 and 250 °c and degradation starts after 300 °c.This outcome has been supported by the previous numerical and experimental studies, propounding the accuracy of preferred modeling and analysis approaches.As the essential distinctness of the research, the effects of elevated temperatures on the bonding behavior between concrete and rebar were considered for numerical analyses.

Journal: ACS omega (2022)
Publication types: Journal Article
A comparison of early fixation of distal radius fractures versus late corrective osteotomy of distal radius malunion.

Fractures of the distal radius are common.Closed reduction and moulded casting is often the first line treatment.Malunion after casting is not uncommon and can lead to discussion on acute surgical fixation versus delayed corrective osteotomy if symptomatic.However, it is unclear if late surgery will provide similar outcomes as early intervention.Methods we performed a single centre, age matched, case series comparison study, comparing outcomes of patients who had undergone early fixation (orif) versus those who had undergone late corrective osteotomy (co) following distal radius fracture.Results twenty-six patients were available for review, 13 in each group.Fracture patterns were similar.Reviewing co versus orif; patients achieved a mean dash; 22 versus 18 (p = 0.355), prwe; 35 versus 26 (p = 0.237), and vas 2 versus 2 (p = 0.490).Grip strength was significantly better in those who had undergone orif; 2% versus -22% (p ≤ 0.001).Range of motion was generally better with primary fixation but of doubtful clinical importance, reviewing co versus orif; flexion 46° versus 60° (p = 0.045), extension 55° versus 64° (p = 0.137), pronation 73° versus 85° (p = 0.078), supination 84° versus 84° (p = 0.747), flexion/extension arc 101 versus 124 (p = 0.017), ulnar/radial deviation arc 42° versus 59° (p = 0.01), pronation/supination arc 157° versus 168° (p = 0.118).Ulnar variance was significantly improved in the orif group; +0.5 mm versus +2 mm in the co group (p = 0.023).Radial inclination, radial height and volar tilt were not significantly different between either group.Conclusion our findings suggest that patient measured outcome of corrective osteotomy is not inferior to early internal fixation.Level of evidence iii (case series comparison).

Journal: ANZ journal of surgery (2022)
Publication types: Journal Article
Bi-layered nanofibrous membrane with osteogenic and antibacterial functions for periodontal tissue regeneration.

Guided tissue regeneration (gtr) membranes have great potential to promote periodontal tissue regeneration and reestablishment.However, the regeneration potential and microbial infection resistance of current gtr membranes still need to be improved.Here, a bi-layered nanofibrous membrane on the basis of poly (lactic-co-glycolic acid) (plga)/gelatin with osteogenic and antibacterial functions was fabricated for periodontal tissue regeneration.The antimicrobial layer (al) of the bi-layered nanofibrous membrane was composed of nanofibrous plga/gelatin nanofibers loaded with nano-silver (n ag), while the osteoconductive layer (ol) of the nanofibrous membrane consisted of plga/gelatin nanofibers loaded with nano-hydroxyapatite (n ha).The bi-layered nanofibrous membrane was examined by scanning electron microscopy (sem), energy dispersive spectrometer (eds), transmission electron microscope (tem), fourier transform infrared spectroscopy (ftir), x-ray photoelectron spectrometry (xps) and x-ray diffractometry (xrd).The results showed that n ha and n ag particles were well evenly loaded or embedded in plga/gelatin nanofibers.The cell culture experiments suggested that the bi-layered nanofibrous membrane possessed good cytocompatibility and the ol of the bi-layered nanofibrous membrane possessed an enhanced osteogenic capacity for human osteoblast-like cells (mg63), which was verified by the good cell viability and the increased alkaline phosphatase (alp) activity, respectively.The results ofin vitroantimicrobial study displayed that the al of the bi-layered nanofibrous membrane possessed an effective antibacterial capability.In conclusion, the prepared bi-layered nanofibrous membrane with osteogenic and antibacterial functions may have great potential for periodontal tissue regeneration and reestablishment.[formula: see text].

Journal: Journal of biomaterials applications (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Impedance analysis of adherent cells after in situ electroporation-mediated delivery of bioactive proteins, DNA and nanoparticles in µL-volumes.

Specific intracellular manipulation of animal cells is a persistent goal in experimental cell biology.Such manipulations allow precise and targeted interference with signaling cascades, metabolic pathways, or bi-molecular interactions for subsequent tracking of functional consequences.However, most biomolecules capable of molecular recognition are membrane impermeable.The ability to introduce these molecules into the cytoplasm and then to apply appropriate readouts to monitor the corresponding cell response could prove to be an important research tool.This study describes such an experimental approach combining in situ electroporation (ise) as a means to efficiently deliver biomolecules to the cytoplasm with an impedance-based, time-resolved analysis of cell status using electric cell-substrate impedance sensing (ecis).In this approach, gold-film electrodes, deposited on the bottom of regular culture dishes, are used for both electroporation and monitoring.The design of the electrode layout and measurement chamber allows working with sample volumes as small as 10 μl.A miniaturized setup for combined electroporation and impedance sensing (μise-ecis) was applied to load different adherent cells with bioactive macromolecules including enzymes, antibodies, nucleic acids and quantum dot nanoparticles.The cell response after loading the cytoplasm with rnase a or cytochrome c (in the presence or absence of caspase inhibitors) was tracked by non-invasive impedance readings in real-time.

Journal: Scientific reports (2020)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Concrete/Glass Construction and Demolition Waste (CDW) Synergies in Ternary Eco-Cement-Paste Mineralogy.

The study described sought further understanding of the synergies in a mix of cdw pozzolans, containing (calcareous and siliceous) concrete and glass waste, used to prepare ternary eco-cement paste bearing 7% of the binary blend at concrete/glass ratios of 2:1 and 1:2.The mineralogical phases in the 2-day, 28-day, and 90-day cement matrices were identified and monitored using xrf, xrd-rietveld, sem-edx, ft-ir, and nmr.The findings showed that changes in the reaction kinetics in the ternary blended pastes relative to opc pastes depended on the nature of the recycled concrete and the glass content.Adding the binary mix bearing calcareous concrete (at a ratio of 2:1) favoured ettringite, portlandite, and amorphous phase formation, whilst the blends with siliceous concrete favoured c-s-h gel formation.Monocarboaluminate was detected in the 90-day siliceous concrete and glass pastes in amounts similar to those found in the reference opc paste.

Journal: Materials (Basel, Switzerland) (2022)
Publication types: Journal Article
Risk Stratification in Noninvasive Respiratory Support Failure: A Narrative Review.

Topic/clinical relevance the use of noninvasive respiratory support, including noninvasive ventilation and high-flow nasal cannula therapy, has increased over the years.Failure of noninvasive respiratory support, defined as the need for invasive mechanical ventilation, increases the mortality rate.Purpose to familiarize critical care nurses with available risk stratification assessments and identify common concepts and limitations.Content covered few risk stratification assessments are available to identify patients at risk of failure of noninvasive respiratory support.Although many studies have analyzed risk, substantial variation in study design, definitions, terminology, and outcomes have led to a wide range of findings, making clinical application difficult.Further study is needed to broaden known assessments to general patient populations, determine diagnostic accuracy during critical periods, and analyze noninvasive ventilation and high-flow nasal cannula therapy.Risk stratification could allow for better implementation of preventive strategies and patient education.Conclusion future research opportunities include improving study design for risk stratification and implementing preventive strategies for patients requiring noninvasive respiratory support.Clinically, risk stratification can provide an opportunity to share knowledge and facilitate conversations with patients and families.

Journal: Critical care nurse (2022)
Publication types: Journal Article;Review
The Mettl3 epitranscriptomic writer amplifies p53 stress responses.

The p53 transcription factor drives anti-proliferative gene expression programs in response to diverse stressors, including dna damage and oncogenic signaling.Here, we seek to uncover new mechanisms through which p53 regulates gene expression using tandem affinity purification/mass spectrometry to identify p53-interacting proteins.This approach identified mettl3, an m6a rna-methyltransferase complex (mtc) constituent, as a p53 interactor.We find that mettl3 promotes p53 protein stabilization and target gene expression in response to dna damage and oncogenic signals, by both catalytic activity-dependent and independent mechanisms.Mettl3 also enhances p53 tumor suppressor activity in in vivo mouse cancer models and human cancer cells.Notably, mettl3 only promotes tumor suppression in the context of intact p53.Analysis of human cancer genome data further supports the notion that the mtc reinforces p53 function in human cancer.Together, these studies reveal a fundamental role for mettl3 in amplifying p53 signaling in response to cellular stress.

Journal: Molecular cell (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural;Research Support, Non-U.S. Gov't
Lasers for the treatment of erythema, dyspigmentation, and decreased elasticity in macular acne scars: a systematic review.

Scarring is one of the most esthetically challenging and psychologically burdening aspects following inflammatory acne.While "macular" disease is the scar subtype with the least complicated outcome, its phase can be regarded as the most defining in the ultimate scar appearance.Moreover, with lasers recently gaining much popularity in the scientific community for managing several dermatologic conditions, we aimed to evaluate whether they would lead to significant benefits.For this systematic review, four databases consisting of pub med, scopus, embase, and web of science were searched using a comprehensive string, with the data from the relevant yet eligible identified records qualitatively synthesized.After investigating the data obtained from the nine included studies, we found the utilized lasers, namely neodymium-doped yttrium aluminum garnet, fractional carbon dioxide, pulsed dye, erbium:glass, pro-yellow, and high-power optically pumped semiconductor, to be highly effective in managing the erythematous or dyspigmented appearance with the reduced elasticity also significantly improving.Moreover, the adverse events were both bearable and minimal, and transient.However, the degree of improvement each type of scar demonstrated following laser therapy varied based on the laser used.Neodymium-doped yttrium aluminum garnet, fractional carbon dioxide, and pulsed dye are the most commonly investigated lasers for managing macular acne scars, demonstrating eye-catching capabilities in managing either erythema or dyspigmentation.However, we still recommend that further comparative interventional studies be carried out, while the intended outcomes also assessed with objective measures for further clarification.

Journal: Lasers in medical science (2022)
Publication types: Systematic Review;Journal Article;Review
Bond Length Alternation and Internal Dynamics in Model Aromatic Substituents of Lignin.

Broadband microwave spectra were recorded over the 2-18 ghz frequency range for a series of four model aromatic components of lignin; namely, guaiacol (ortho-methoxy phenol, g), syringol (2,6-dimethoxy phenol, s), 4-methyl guaiacol (mg), and 4-vinyl guaiacol (vg), under jet-cooled conditions in the gas phase.Using a combination of13c isotopic data and electronic structure calculations, distortions of the phenyl ring by the substituents on the ring are identified.In all four molecules, the r c(1)-c(6)bond between the two substituted c-atoms lengthens, leading to clear bond alternation that reflects an increase in the phenyl ring resonance structure with double bonds at r c(1)-c(2), r c(3)-c(4)and r c(5)-c(6).Syringol, with its symmetric methoxy substituents, possesses a microwave spectrum with tunneling doublets in the a-type transitions associated with h-atom tunneling.These splittings were fit to determine a barrier to hindered rotation of the oh group of 1975 cm-1, a value nearly 50 % greater than that in phenol, due to the presence of the intramolecular oh⋅⋅⋅och3h-bonds at the two equivalent planar geometries.In 4-methyl guaiacol, methyl rotor splittings are observed and used to confirm and refine an earlier measurement of the three-fold barrier v3=67 cm-1.Finally, 4-vinyl guaiacol shows transitions due to two conformers differing in the relative orientations of the vinyl and oh groups.

Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Changes in Cost-Effectiveness for Chronic Hepatitis C Virus Pharmacotherapy: The Case for Continuous Cost-Effectiveness Analyses.

Cost-effectiveness evaluations for hepatitis c virus (hcv) treatments have been published frequently, but new products with significant cost and effectiveness differences make these analyses obsolete.How valuable are economic models for a fixed time period in a dynamic market?Objective to estimate the cost-effectiveness of the best available hcv treatment at different points in time, using the same comparator to demonstrate how rapid innovation in a disease area influences economic outcomes.Methods a markov model was used to calculate the cost-effectiveness of treatment in 2010, 2012, 2014, 2016, and 2018 compared with a standard comparator (no treatment) from the payer perspective.Expected drug costs and treatment effectiveness estimates for sustained virologic response (svr) were calculated using recommended regimens for each of the 6 hcv genotypes at each time point and distribution of genotypes in the united states.Patients entered the model with different stages of fibrosis.Utility estimates for each health state were used to calculate quality-adjusted life-years (qalys) earned at each cycle.Incremental cost-effectiveness ratios were reported for each year to compare the "treatment versus no treatment" decision at that time.Results no hcv treatment resulted in a gain of 11.54 qalys over a 20-year time horizon at a cost of $42,938.Costs for treated groups were $69,075, $123,267, $125,431, $86,782, and $56,470 for the 2010, 2012, 2014, 2016, and 2018 scenarios, respectively.Qalys gained for treated groups were 12.90, 12.97, 13.34, 13.39, and 13.46 for the 2010, 2012, 2014, 2016, and 2018 scenarios, respectively.The incremental cost-effectiveness ratios in each year compared with no treatment were $19,218 per qaly, $56,104 per qaly, $45,829 per qaly, $23,699 per qaly, and $7,048 per qaly.Conclusions treatment effectiveness for hcv has increased steadily, while treatment costs increased substantially from 2010-2014 before decreasing to its lowest point in 2018.Thus, the dynamic nature of innovation creates the need for iterative cost-effectiveness analyses.Disclosures no outside funding supported this study.Mattingly reports unrelated consulting from the national health council, bristol myers squibb, g&w laboratories, allergy and asthma foundation of american, and the massachusetts health policy commission.Love reports an unrelated research grant from the american cancer society.

Journal: Journal of managed care & specialty pharmacy (2020)
Publication types: Journal Article
Clinical characteristics and comorbidities associated with testosterone prescribing in men.

Testosterone replacement therapy (trt) is recommended for the treatment of symptomatic hypogonadism in men.Data on prescription behaviours are, however, limited and conflicting.The objective of this study was to investigate clinical characteristics associated with the likelihood of being prescribed trt by general practitioners (gp) in north-west london (nwl).Design retrospective cohort study using discover database of gp-registered patients in nwl between 2015 and 2019.Patients we identified 20,299 men aged ≥18 years with serum total testosterone measurement (tt) and without prior trt prescription records.Measurements we determined whether trt was subsequently commenced, while analysing clinical characteristics related to hypogonadism.Results of all men having tt measurement, 19,583 (96.4%) were not commenced on trt (group a) and 716 (3.5%) men were commenced on trt (group b).Men prescribed trt (group b) had higher mean age, body mass index (bmi) and higher risks of hypertension, depression type 2 diabetes and ischaemic heart disease; conversely, men in group b had lower mean pretreatment tt and were less likely to have prostate cancer.Four-hundred and thirty-six men (24.3%) with tt < 8 nmol/l and symptoms of low libido were not prescribed trt.Conclusions our study highlights several factors which may influence the decisions made by clinicians when initiating trt in primary care.Clearer guidance for clinicians may help to improve the consistency of treatment of men with hypogonadism.

Journal: Clinical endocrinology (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Licochalcone A-Induced Apoptosis Through the Activation of p38MAPK Pathway Mediated Mitochondrial Pathways of Apoptosis in Human Osteosarcoma Cells In Vitro and In Vivo.

Licochalcone a (lic a) is isolated from the roots of glycyrrhiza glabraand possesses antitumor and anti-invasive activities against several tumor cells.However, the antitumor effects of lic a on human osteosarcoma cells have yet to be demonstrated either in vitro or in vivo.Methods cell viability was measured by mtt assay.Apoptosis and mitochondrial dysfunction were detected with annexin v/pi staining and jc-1 staining by flow cytometry.The expressions of caspase- or mitochondrial-related proteins were demonstrated by western blotting.Antitumor effect of lic a on 143b xenograft mice in vivo.Results lic a could inhibit cell proliferation and induce apoptosis in human osteosarcoma cells, as evidenced by a decrease in cell viability, loss of mitochondrial membrane potentials, and activation of caspases.Lic a treatment substantially reduced the expression of bcl-2 and mcl-1 and increased the expression of cleaved-caspase-3, cleaved-caspase-9, cleaved-parp, and bax in hos and u2os cells.Moreover, mitochondrial membrane potential and apoptosis suppression mediated by z-vad or tauroursodeoxycholic acid significantly reduced lic a-induced mitochondria-dependent apoptosis.The study also determined that lic a treatment induced p38mapk phosphorylation, but si rna-p38 or birb796 substantially reversed cell viability through the inhibition of mitochondria-dependent apoptosis pathways.Finally, an in vivo study revealed that lic a significantly inhibited 143b xenograft tumor growth.Conclusions these findings demonstrate that lic a has antitumor activities against human osteosarcoma cells through p38mapk regulation of mitochondria-mediated intrinsic apoptotic pathways in vitro and in vivo.

Journal: Cells (2019)
Publication types: Journal Article;Research Support, Non-U.S. Gov't