syedzayyan/paper_mill_benchmark huggingface dataset:
Lower expression of PDZRN3 induces endometrial carcinoma progression via the activation of canonical Wnt signaling.

Endometrial carcinoma (ec) exhibits an extremely malignant biological behavior and has a high mortality rate.Ec has recently become one of the most lethal cancers in women worldwide.E3 ubiquitin ligases play an important role in the biological function of healthy cells but can also contribute to tumorigenesis and cancer development.Pdz domain containing ring finger 3 (pdzrn3) is associated with cell differentiation and its structure includes the e3 ubiquitin ligase.However, the effects of pdzrn3 in ec remain unclear.Reverse transcription-quantitative pcr was used to detect the expression levels of pdzrn3 in ec cells, and the role of pdzrn3 in ec progression was determined using western blotting, mtt, colony formation, transwell, subcutaneous tumor formation and pulmonary metastasis assays.A multi-pathway reporter arrays and western blotting were performed to investigate the potential biological mechanisms of pdzrn3 in ec.The present study demonstrated that pdzrn3 served an essential role in metastasis and proliferation of ec.Pdzrn3 expression was lower in ec tissues compared with that in normal endometrial tissues.Low expression level of pdzrn3 in ec was correlated with certain clinicopathological features of patients with ec, such as the age of the patients, the tumor grade and the tumor subtype.The invasive and proliferative activities of ec cells with low expression of pdzrn3 were more potent than those of ec cells with high expression of pdzrn3, which was confirmed by in vivo and in vitro experiments.Furthermore, lower expression of pdzrn3 promoted metastasis and proliferation via activation of the canonical wnt signaling pathway.The present study demonstrated that decreased pdzrn3 expression promoted metastasis and proliferation in ec cells via activation of the canonical wnt signaling pathway, highlighting a potential biological therapeutic target for the management of ec.

Journal: Oncology letters (2022)
Publication types: Journal Article
Survey on Sildenafil, Tadalafil, and Vardenafil Concentrations in Food Supplements for Erectile Dysfunction.

The aim of the study was to analyze the presence of undeclared sildenafil, tadalafil, and vardenafil in food supplements (fss) for erectile dysfunction.The presence of sildenafil, tadalafil, and vardenafil was determined using the generated ultraviolet (uv)-spectra and mass-spectrometry (ms)-spectra as well as chromatograms produced by the photodiode array (pda)-detector and ion trap ms-detector.The results were processed by xcalibur ver.2.0.7.Fourteen of the 20 analyzed fss contained undeclared ingredients.Sildenafil was present in 12 of them.Many violations and discrepancies between the label information and the real composition of the fs were identified.70% of the samples contained undeclared ingredients of an erectile dysfunction medicinal product.The quantities varied within broad limits from 2 mg per tablet to 116.55 mg per tablet.Sildenafil was present in amounts exceeding 16.55 mg that is the maximum recommended dose, thus creating risk of overdose.Besides that, food supplements adulterated with sildenafil analogues are a health risk for consumer's health as there is no evidence of modified sildenafil toxicity.All analyzed fss were claimed to be 100% natural, not provoking side effects.No information for any fs contained indications of age limits or risk for interaction with other fss or medical products.

Journal: International journal of analytical chemistry (2022)
Publication types: Journal Article
Hematologic concerns in extracorporeal membrane oxygenation.

This isth "state of the art" review aims to critically evaluate the hematologic considerations and complications in extracorporeal membrane oxygenation (ecmo).Ecmo is experiencing a rapid increase in clinical use, but many questions remain unanswered.The existing literature does not address or explicitly state many pertinent details that may influence hematologic complications and, ultimately, patient outcomes.This review aims to broadly introduce modern ecmo practices, circuit designs, circuit materials, hematologic complications, transfusion-related considerations, age- and size-related differences, and considerations for choosing outcome measures.Relevant studies from the 2019 isth congress in melbourne, which further advanced our understanding of these processes, will also be highlighted.

Journal: Research and practice in thrombosis and haemostasis (2020)
Publication types: Journal Article;Review
Compression-induced crimping of boron nanotubes from borophenes: a DFT study.

Several borophenes have been prepared successfully, but the synthesis of boron nanotubes is still very difficult.Our results suggest that the high flexibility of borophene in combination with van der waals interactions makes it possible to coil boron nanotubes from rippled borophenes, which is confirmed byab initiomolecular dynamics simulations.The plane structures transform into rippled structures almost without any barrier under very small compression and weak perturbations like molecular adsorption.The compression energies of the rippled structures increase linearly and slowly with the increase of the compression.This suggests how the geometry of the borophene evolves with compression.Based on the evaluation of the free energy of hydrogen adsorption, a stronger compression suggests the improved hydrogen evolution performance of the borophene and even makes it better than pt catalysts.Meanwhile, good hydrogen evolution performance is also suggested for boron nanotubes.Our results suggest a novel preparation method for boron nanotubes from borophenes and a possible way to improve their hydrogen evolution performance.

Journal: Physical chemistry chemical physics : PCCP (2022)
Publication types: Journal Article
Four-in-One Stimulus-Responsive Long-Lived Luminescent Systems Based on Pyrene-Doped Amorphous Polymers.

Materials exhibiting ultralong luminescent lifetime show promising applications in the fields of information encryption, sensing, and bioimaging.Herein, we present a low-cost and general strategy to achieve stimulus-responsive ultralong organic phosphorescence (uop) based on pyrene chromophores doped into polymer matrices.The uop of the resulted systems presents radiation-, concentration-, time-, and excitation-dependent characteristics.The uop color can be turned from blue to red by changing the excitation wavelength or the concentration of chromophores.Experimental results prove that these characteristics are attributed to the consumption of triplet oxygen and the different aggregation states of chromophores in the polymer matrices.Finally, we demonstrate that these systems could be applied for multilevel information encryption.This work would promote further development of multi-responsive long-lived luminescent materials.

Journal: Angewandte Chemie (International ed. in English) (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Imaging Features of Hepatocellular Carcinoma With Bile Duct Tumor Thrombus: A Multicenter Study.

S there are still challenging problems in diagnosis of hepatocellular carcinoma (hcc) with bile duct tumor thrombus (bdtt) before operation.This study aimed to analyze the imaging features of hcc with b1-b3 bdtt.Materials and methods the clinicopathological data and imaging findings of 30 hcc patients with b1-b3 bdtt from three high-volume institutions were retrospectively reviewed.A total of 631 patients without bdtt who were randomly collected from each of the enrolled centers were recorded as the control group to analyze the differences in clinicopathological characteristics and imaging features between the two groups.A total of 453 hcc patients who underwent surgical treatment in the three institutions from january 2020 to december 2020 were collected for a blinded reading test as the validation group.Results hcc patients with b1-b3 bdtt had more advanced tumor stages and adverse clinicopathological features.Hcc lesions were detected in all patients, and intrahepatic bile duct dilation was observed in 28 (93.3%) patients with b1-b3 bdtt and 9 (1.43%) patients in hcc without bdtt.The intrahepatic bile duct dilation showed no enhancement at hepatic arterial phase (hap) and no progressively delayed enhancement at portal venous phase (pvp), but it was more obvious at pvp on ct.In the reports of the 30 hcc patients with b1-b3 bdtt generated for the image when the scan was done, bdtt was observed in all 13 b3 patients and 3 of 12 b2 patients, but none of the 5 b1 patients.Fourteen patients were misdiagnosed before surgery.However, when using intrahepatic bile duct dilation in hcc patients as a potential biomarker for bdtt diagnosis, the sensitivity and specificity for bdtt diagnosis were 93.33% and 98.57%, respectively.The blinded reading test showed that intrahepatic bile duct dilation in ct and mri scans could be for separating hcc patients with b1-b3 bdtt from hcc patients without bdtt.Conclusions the hcc lesions and intrahepatic bile duct dilation on ct or mri scans are imaging features of hcc with bdtt, which might facilitate the early diagnosis of b1-b3 bdtt.

Journal: Frontiers in oncology (2021)
Publication types: Journal Article
Citrullination as a novel posttranslational modification of matrix metalloproteinases.

Matrix metalloproteinases (mmps) are enzymes with critical roles in biology and pathology.Glycosylation, nitrosylation and proteolysis are known posttranslational modifications (ptms) regulating intrinsically the activities of mmps.We discovered mmp citrullination by peptidyl arginine deiminases (pads) as a new ptm.Upon hypercitrullination, mmp-9 acquired a higher affinity for gelatin than control mmp-9.Furthermore, hypercitrullinated pro mmp-9 was more efficiently activated by mmp-3 compared to control mmp-9.Jnj0966, a specific therapeutic inhibitor of mmp-9 activation, inhibited the activation of hypercitrullinated pro mmp-9 by mmp-3 significantly less in comparison with control pro mmp-9.The presence of citrullinated/homocitrullinated mmp-9 was detected in vivo in neutrophil-rich sputum samples of cystic fibrosis patients.In addition to citrullination of mmp-9, we report efficient citrullination of mmp-1 and lower citrullination levels of mmp-3 and mmp-13 by pad2 in vitro.In conclusion, citrullination of mmps is a new ptm worthy of additional biochemical and biological studies.

Journal: Matrix biology : journal of the International Society for Matrix Biology (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Early impairments in the retina of rats fed with high fructose/high fat diet are associated with glucose metabolism deregulation but not dyslipidaemia.

Way of life changes such as high consumption of processed foods rich in fat and sugar and sedentary lifestyle are associated with the increasing prevalence of metabolic syndrome (met s) that affects about 35% in the american population.Met s is the main risk factor for diabetes mellitus, which is associated with vascular changes in the retina.However, the early consequences of met s in the retina are not well described.We therefore aimed at characterizing the early effects of a high fructose and high fat diet (hfhf) on the function and structure of the rat retina, and evaluate the associations with metabolic changes.Brown norway rats of 6 weeks of age were fed for 8 days, 5 weeks or 13 weeks with hfhf diet, or a standard chow.After only 4 weeks of this diet, rats exhibited a reduction in cone photoreceptor sensitivity to light.Moreover, we observed that met s significantly exacerbated laser-induced choroidal neovascularization by 72% and 67% 2 weeks and 3 weeks post laser treatment, respectively.These retinal abnormalities were associated with deregulation of glucose metabolism but not lipid metabolism.These data showed retinal modifications in hfhf-induced met s in the rat, at very early stage of the disease.

Journal: Scientific reports (2019)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
The role of material deprivations in determining ART adherence: Evidence from a conjoint analysis among HIV-positive adults in Uganda.

Despite sustained global scale-up of antiretroviral therapy (art), adherence to art remains low.Less than half of those in hiv care in uganda achieve 85% adherence to their art medication required for clinically meaningful viral suppression, leaving them at higher risk of transmission.Key barriers to art adherence include poverty-related structural barriers that are inter-connected and occur simultaneously, making it challenging to examine and disentangle them empirically and in turn design effective interventions.Many people living with hiv (plwh) make tradeoffs between these various barriers (e.g., between expenses for food or transportation) and these can influence long-term health behavior such as adherence to art.To be able to estimate the distinct influence of key structural barriers related to poverty, we administered a conjoint analysis (ca) to 320 hiv-positive adults currently taking art at an urban clinic in uganda between july 2019 and september 2020.We varied the levels of four poverty-related attributes (food security, sleep deprivation, monthly income, and physical pain) that occur simultaneously and asked respondents how they would adhere to their medication under different combinations of attribute levels.This allows us to disentangle the effect of each attribute from one another and to assess their relative importance.We used regression analysis to estimate the effects of each attribute level and found that food security impacts expected adherence the most (treatment effect = 1.3; 95% ci 1.11-1.49, p<0.001), followed by income (treatment effect = 0.99; 95% ci 0.88-1.10, p<0.001.Sleep and pain also impact adherence, although by a smaller magnitude.Sub-group analyses conducted via regression analysis examine heterogeneity in results and suggest that the effects of material deprivations on expected adherence are greater among those with high levels of existing food insecurity.Results from this ca indicate that external factors inherent in the lives of the poor and unrelated to direct art access can be important barriers to art adherence.This study applies a ca (typically administered in marketing applications) among plwh to better understand individual-level perceptions relating to poverty that often occur simultaneously.Policy interventions should address food insecurity and income to improve adherence among hiv-positive adults.

Journal: PLOS global public health (2022)
Publication types: Journal Article
Financial risk of road traffic trauma care in public and private hospitals in Addis Ababa, Ethiopia: A cross-sectional observational study.

Road traffic injuries are among the most important causes of morbidity and mortality and cause substantial economic loss to households in ethiopia.This study estimates the financial risks of seeking trauma care due to road traffic injuries in addis ababa, ethiopia.Methods this is a cross-sectional survey on out-of-pocket (oop) expenditures related to trauma care in three public and one private hospital in addis ababa from december 2018 to february 2019.Direct medical and non-medical costs (2018 usd) were collected from 452 trauma cases.Catastrophic health expenditures were defined as oop health expenditures of 10% or more of total household expenditures.Additionally, we investigated the impoverishment effect of oop expenditures using the international poverty line of $1.90 per day per person (adjusted for purchasing power parity).Results trauma care seeking after road traffic injuries generate catastrophic health expenditures for 67% of households and push 24% of households below the international poverty line.On average, the medical oop expenditures per patient seeking care were $256 for outpatient visits and $690 for inpatient visits per road traffic injury.Patients paid more for trauma care in private hospitals, and oop expenditures were six times higher in private than in public hospitals.Transport to facilities and caregiver costs were the two major cost drivers, amounting to $96 and $68 per patient, respectively.Conclusion seeking trauma care after a road traffic injury poses a substantial financial threat to ethiopian households due to lack of strong financial risk protection mechanisms.Ethiopia's government should enact multisectoral interventions for increasing the prevention of road traffic injuries and implement universal public finance of trauma care.

Journal: Injury (2022)
Publication types: Journal Article;Observational Study
Cellular and Subcellular Characteristics of Neuromuscular Junctions in Muscles with Disparate Duty Cycles and Myofiber Profiles.

The neuromuscular system accounts for a large portion (~40%) of whole body mass while enabling body movement, including physical work and exercise.At the core of this system is the neuromuscular junction (nmj) which is the vital synapse transducing electrical impulses from the motor neurons to their post-synaptic myofibers.Recent findings suggest that subcellular features (active zones) of the nmj are distinctly sensitive to changes in activity relative to cellular features (nerve terminal branches, vesicles, receptors) of the nmj.In the present investigation, muscles with different recruitment patterns, functions, and myofiber type profiles (soleus, plantaris, extensor digitorum longus [edl]) were studied to quantify both cellular and subcellular nmj characteristics along with myofiber type profiles.Results indicated that, in general, dimensions of subcellular components of nmjs mirrored cellular nmj features when examining inter-muscle nmj architecture.Typically, it was noted that the nmjs of the soleus, with its most pronounced recruitment pattern, were larger (p< 0.05) than nmjs of less recruited muscles.Moreover, it was revealed that myofiber size did not dictate nmj size as soleus muscles displayed the smallest fibers (p< 0.05) while the plantaris muscles exhibited the largest fibers.In total, these data show that activity determines the size of nmjs and that generally, size dimensions of cellular and subcellular components of the nmj are matched, and that the size of nmjs and their underlying myofibers are uncoupled.

Journal: Cells (2023)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
A Comparative Genomic and Safety Assessment of SixLactiplantibacillus plantarumsubsp.argentoratensisStrains Isolated from Spontaneously Fermented Greek Wheat Sourdoughs for Potential Biotechnological Application.

The comparative genome analysis of six lactiplantibacillus plantarumsubsp.argentoratensisstrains previously isolated from spontaneously fermented greek wheat sourdoughs is presented.Genomic attributes related to food safety have been studied according to the european food safety authority (efsa) suggestions for the use of lactic acid bacteria (lab) in the production of foods.Bioinformatic analysis revealed a complete set of genes for maltose, sucrose, glucose, and fructose fermentation; conversion of fructose to mannitol; folate and riboflavin biosynthesis; acetoin production; conversion of citrate to oxaloacetate; and the ability to produce antimicrobial compounds (plantaricins).Pathogenic factors were absent but some antibiotic resistance genes were detected.Crispr andcasgenes were present as well as various mobile genetic elements (mges) such as plasmids, prophages, and insertion sequences.The production of biogenic amines by these strains was not possible due to the absence of key genes in their genome except lysine decarboxylase associated with cadaverine; however, potential degradation of these substances was identified due to the presence of a blue copper oxidase precursor and a multicopper oxidase protein family.Finally, comparative genomics and pan-genome analysis showed genetic differences between the strains (e.g., variableplnlocus), and it facilitated the identification of various phenotypic and probiotic-related properties.

Journal: International journal of molecular sciences (2022)
Publication types: Journal Article
The Fundamental Tension in Integrated Information Theory 4.0's Realist Idealism.

Integrated information theory (iit) is currently one of the most influential scientific theories of consciousness.Here, we focus specifically on a metaphysical aspect of the theory's most recent version (iit 4.0), what we may call itsidealistic ontology, and its tension with a kind ofrealismabout the external world that iit also endorses.Iit 4.0 openly rejects the mainstream view that consciousness is generated by the brain, positing instead that consciousness is ontologically primary while the physical domain is just "operational".However, this philosophical position is presently underdeveloped and is not rigorously formulated in iit, potentially leading to many misinterpretations and undermining its overall explanatory power.In the present paper we aim to address this issue.We argue that iit's idealistic ontology should be understood as a specific combination of phenomenal primitivism, reductionism regardingφ-structures and complexes, and eliminativism about non-conscious physical entities.Having clarified this, we then focus on the problematic tension between iit's idealistic ontology and its simultaneous endorsement of realism, according to which there is some kind of external reality independent of our minds.After refuting three potential solutions to this theoretical tension, we propose the most plausible alternative: understanding iit's realism as an assertion of the existence of other experiences beyond one's own, what we call anon-solipsistic idealist realism.We end with concluding remarks and future research avenues.

Journal: Entropy (Basel, Switzerland) (2023)
Publication types: Journal Article
Gut microbiota as the critical correlation of polycystic ovary syndrome and type 2 diabetes mellitus.

Gut microbiota forms a symbiotic relationship with the host and maintains the ecological balance of the internal and external environment of the human body.However, dysbiosis of the gut microbiota and immune deficiency, as well as environmental changes, can destroy the host-microbial balance, leading to the occurrence of a variety of diseases, such as polycystic ovary syndrome (pcos), type 2 diabetes mellitus (t2dm), and obesity.Meanwhile, diseases can also affect gut microbiota, forming a vicious cycle.The role of the intestinal microbiota in different diseases have been proven by several studies; however, as a common target of pcos and t2dm, there are few reports on the treatment of different diseases through the regulation of intestinal microbiota as the critical correlation.This review analyzed the common mechanisms of intestinal microbiota in pcos and t2dm, including the dysbiosis of gut microbiota, endotoxemia, short-chain fatty acids, biotransformation of bile acids, and synthesis of amino acid in regulating insulin resistance, obesity, chronic inflammation, and mitochondrial dysfunction.The possible therapeutic effects of probiotics and/or prebiotics, fecal microbiota transplantation, bariatric surgery, dietary intervention, drug treatment, and other treatments targeted at regulating intestinal microbiota were also elucidated.

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (2021)
Publication types: Journal Article;Review