syedzayyan/paper_mill_benchmark huggingface dataset:
Recent developments in understanding the length dependence of contractile response of skeletal muscle.

Maximal active force of skeletal muscle contraction occurs at a sarcomere length where overlap of thick and thin filaments is optimal.However, the interaction of muscle length and active force is complicated.Active force, is the force generated by energy-requiring processes.To calculate active force, passive force provided by in-parallel structures must be subtracted from total force.Sarcomere length will change during a contraction with constant muscle-tendon length, due to tendon stretch.Passive force therefore changes during the contraction.Taking this into account, it has been demonstrated that there is less length dependence of fatigue than previously thought.The remaining difference may be associated with length dependence of activation, a property that is evident with submaximal activation.The sarcomere length at which peak contraction occurs is longer than the length that gives optimal overlap of the filaments and this shift of optimal length appears to be due to increased ca2+sensitivity.The increased ca2+sensitivity occurs because at longer lengths, the myofilaments are closer together allowing greater force than expected.However, the potential for length-dependent activation has been challenged.Submaximal contractions obtained by recruitment of fewer motor units but with maximal stimulation across different muscle lengths still demonstrate length-dependent activation.In contrast, contractions with similar absolute electromyographic signal magnitude at different lengths do not demonstrate length-dependent activation.Recent work has improved our understanding of how sarcomere length impacts the force of contraction but also reveals inadequacies in our knowledge that need to be addressed by additional research.

Journal: European journal of applied physiology (2017)
Publication types: Journal Article;Review
Role of micro-RNA132 and its long non coding SOX2 in diagnosis of lupus nephritis.

The skin and the kidney are commonly affected in systemic lupus erythematosus (sle) with similar molecular mechanisms.Although clinical indicators of renal injury in sle are fairly uncontroversial, few biomarkers are reliable.The role of micro-rnas (mi-rnas) in lupus nephritis (ln) pathogenesis has been investigated to help in early diagnosis.Purpose the aim of work is to evaluate mi rna132 and sox2 expressions in sle egyptian patients; with and without nephritis, and the relation between mi rna132 and its long non-coding gene sox2 in both patients groups.Research design this is a case-control study involving 100 sle patients with and without ln (ln and non-ln groups), and 50 age-and sex-matched healthy controls.The study was carried out to detect mi rna132 and sox2 expression by quantitative real-time polymerase chain reaction methods.The sle disease activity index (sledai) was assessed.Results sledai increased in ln compared to non-ln.Micro-rna132 expression was significantly increased in patient groups compared to controls (p<0.01) and increased in ln more than non-ln group (p<0.001).Sox2 significantly decreased in patient groups compared to controls (p<0.001), and was more in ln compared to non-ln group (p<0.001).There was a negative correlation between mi rna132 and sox2 expression in both patient groups (p<0.001).Conclusion mi rna132 and sox2 may play a role in sle activity and help in the early non-invasive diagnosis of ln.

Journal: Lupus (2022)
Publication types: Journal Article
Metabolic and lipidomic characterization of radioresistant MDA-MB-231 human breast cancer cells to investigate potential therapeutic targets.

To provide preliminary insights into metabolic and lipidomic characteristics in radioresistant triple-negative breast cancer (tnbc) cells and suggest potential therapeutic targets, we performed a comprehensive metabolic and lipidomic profiling of radioresistant mda-mb-231 (mda-mb-231/rr) tnbc cells and their parental cells using gas chromatography-mass spectrometry and nano electrospray ionization-mass spectrometry, followed by multivariate statistical analysis.Buthionine sulfoximine (bso) and radiation were co-treated to radioresistant tnbc cells.The level of glutathione (gsh) was significantly increased, and the levels of gsh synthesis-related metabolites, such as cysteine, glycine, and glutamine were also increased in mda-mb-231/rr cells.In contrast, the level of lactic acid was significantly reduced.In addition, reactive oxygen species (ros) level was decreased in mda-mb-231/rr cells.In the lipidomic profiles of mda-mb-231/rr cells, the levels of phosphatidylcholine (pc) and phosphatidylethanolamine (pe) were significantly increased, whereas those of most of the phosphatidylinositol species were significantly decreased.Bso sensitized mda-mb-231/rr cells to radiotherapy, which resulted in decreased gsh level and increased ros level and apoptosis.Radioresistant tnbc cells showed distinct metabolic and lipidomic characteristics compared to their parental cells.We suggested activated gsh, pc, and pe biosynthesis pathways as potential targets for treating radioresistant tnbc cells.Particularly, enhanced radiosensitivity was achieved by inhibition of gsh biosynthesis in mda-mb-231/rr cells.

Journal: Journal of pharmaceutical and biomedical analysis (2022)
Publication types: Journal Article
Calcium intake in an adult Ukrainian population.

This is a first large epidemiological study of calcium intake in ukrainian population.The data from 1518 outpatient subjects aged 20-89 years old showed that the mean level of calcium intake composed 615.6 mg/day (735.4 mg/day in males and 565.6 mg/day in females, p < 0.0001) without any significant differences according to age.Introduction insufficiency in calcium (ca) intake is a worldwide problem; however, corresponding data regarding ukrainian population is limited.This research was aimed to investigate the level of ca consumption in daily rations of ukrainian adults depending on age and sex.Methods the data from 1518 outpatient subjects from urban and rural areas of ukraine were analyzed; the ca intake was evaluated by the method of 24-h dietary recalls during three non-consecutive days by a trained dietitian with further computer calculation.Results the mean level of ca intake in ukrainian population composed 615.6 mg/day (735.4 mg/day in males and 565.6 mg/day in females) with significantly higher level of consumption in men than in women in whole group and in the subjects aged 50 years and more.In total, 67% of men and 78% of women consumed less than 800 mg/day ca and only 28% of males and 10% of females consumed more than 1000 mg/day ca.Conclusions this is a first large epidemiological study of ca intake in ukrainian population which confirmed its low level.This insufficiency in ca intake can have a negative influence on exaggerated bone loss, osteoporosis development, and fracture risk that require forehanded correction.

Journal: Archives of osteoporosis (2020)
Publication types: Journal Article
Optimizing the Synthetic Conditions of "Green" Colloidal AgBiS2Nanocrystals Using a Low-Cost Sulfur Source.

Colloidal ag bi s2nanocrystals (ncs) have attracted increasing attention as a near-infrared absorbent materials with non-toxic elements and a high absorption coefficient.In recent years, colloidal ag bi s2ncs have typically been synthesized via the hot injection method using hexamethyldisilathiane (tms) as the sulfur source.However, the cost of tms is one of the biggest obstacles to large-scale synthesis of colloidal ag bi s2ncs.Herein, we synthesized colloidal ag bi s2ncs using oleylamine@sulfur (ola-s) solution as the sulfur source instead of tms and optimized the synthesis conditions of colloidal ag bi s2ncs.By controlling the reaction injection temperature and the dosage of ola-s, colloidal ag bi s2ncs with adjustable size can be synthesized.Compared with tms-based colloidal ag bi s2ncs, the colloidal ag bi s2ncs based on ola-s has good crystallinity and fewer defects.

Journal: Nanomaterials (Basel, Switzerland) (2022)
Publication types: Journal Article
Pre-Acclimation to Elevated Temperature Stabilizes the Activity of Photosystem I in Wheat Plants Exposed to an Episode of Severe Heat Stress.

The importance of high temperature as an environmental factor is growing in proportion to deepening global climate change.The study aims to evaluate the effects of long-term acclimation of plants to elevated temperature on the tolerance of their photosynthetic apparatus to heat stress.Three wheat (triticumsp.L.) genotypes differing in leaf and photosynthetic traits were analyzed: thesee, roter samtiger kolbenweizen, and ank 32a.The pot experiment was established in natural conditions outdoors (non-acclimated variant), from which a part of the plants was placed in foil tunnel with elevated temperature for 14 days (high temperature-acclimated variant).A severe heat stress screening experiment was induced by an exposition of the plans in a growth chamber with artificial light and air temperature up to 45 °c for ~12 h before the measurements.The measurements of leaf photosynthetic co2assimilation, stomatal conductance, and rapid kinetics of chlorophyll a fluorescence was performed.The results confirmed that a high temperature drastically reduced the photosynthetic assimilation rate caused by the non-stomatal (biochemical) limitation of photosynthetic processes.On the other hand, the chlorophyll fluorescence indicated only a moderate level of decrease of quantum efficiency of photosystem (ps) ii (fv/fm parameter), indicating mostly reversible heat stress effects.The heat stress led to a decrease in the number of active ps ii reaction centers (rc/abs) and overall activity o psii (piabs) in all genotypes, whereas the ps i (parameter ψreo) was negatively influenced by heat stress in the non-acclimated variant only.Our results showed that the genotypes differ in acclimation capacity to heat stress, and rapid noninvasive techniques may help screen the stress effects and identify more tolerant crop genotypes.The acclimation was demonstrated more at the ps i level, which may be associated with the upregulation of alternative photosynthetic electron transport pathways with clearly protective functions.

Journal: Plants (Basel, Switzerland) (2022)
Publication types: Journal Article
Sex difference in response to non-small cell lung cancer immunotherapy: an updated meta-analysis.

Studying sex differences in the efficacy of immunotherapy may contribute to the practice of the precision medicine, especially in non-small cell lung cancer (nsclc), a kind of cancer with sexual bimorphism.Methods published randomized controlled trials (rcts), published by pub med, medline, embase, and scopus, before 15 june 2022, testing immunotherapy (ctla-4 or pd-1/l1 inhibitor alone, combination or with chemotherapy) versus non-immunotherapy (receiving chemotherapy or placebo only) were included to assess different efficacy between males and females.The primary endpoint was overall survival (os).This meta-analysis was registered with prospero (crd42022298439).Results sixteen rcts, involving 10,155 patients with advanced nsclc, was collected in this meta-analysis.The pooled hr comparing immunotherapy vs non-immunotherapy were 0.76 (95%ci 0.71-0.81) for males and 0.74 (95%ci 0.63-0.87) for females.The pooled hrs comparing immune-checkpoint inhibitors (icis) plus chemotherapy versus chemotherapy were 0.79 (95%ci 0.70-0.89) for males and 0.63 (95%ci 0.42-0.92) for females.The pooled hrs comparing icis versus chemotherapy were 0.74 (95%ci 0.67-0.81) for males and 0.83 (95%ci 0.73-0.95) for females.In squamous nsclc, the pooled hrs comparing immunotherapy vs non-immunotherapy were 0.73 (95%ci 0.58-0.91) for males and 0.74 (95%ci 0.37-1.48) for females.In non-squamous nsclc, the pooled hrs comparing immunotherapy versus non-immunotherapy were 0.62 (95%ci 0.71-0.94) for males and 0.59 (95%ci 0.39-0.89) for females.Conclusion compared to chemotherapy, immunotherapy can improve the prognosis of patients with advanced nsclc.Meanwhile, there are sex differences in the efficacy of immunotherapy.key messagecompared to chemotherapy, immunotherapy can improve the prognosis of patients with advanced nsclc.the most interesting thing in this study is that immunotherapy showed significant sex differences in the treatment of squamous nsclc.

Journal: Annals of medicine (2022)
Publication types: Meta-Analysis;Systematic Review;Journal Article
Compliance of NKF KDOQI 2020 nutrition guideline recommendations with other guideline recommendations and protein energy wasting criteria in hemodialysis patients.

Introduction nutrition in hemodialysis patients is important in decreasing complications, improving quality of life, and preventing of malnutrition.Recommendations of the guidelines are taken into consideration while prescribing a nutrition therapy plan for patients.However, the recommendations may differ between the guidelines.It was aimed to compare the newly published national kidney foundation kidney disease outcomes quality initiative (nkf kdoqi) guideline with previous reported two guideline recommendations and protein energy wasting criteria in this study.Materials and methods fifty-five maintenance hemodialysis patients between the ages of 18-65 were included in the study.Daily energy intake and daily protein intake of these patients were evaluated by three different nutrition guidelines; nkf kdoqi-2000, 2020 and european best practice guidelines-2007.In addition, protein energy wasting was determined by using anthropometric measurements, biochemical findings and food intake of the patients.Results when the inadequacy rate in dietary daily energy intake and daily protein intake of the patients evaluated by nkf kdoqi-2000 recommendations, it was found to be higher than the rates in other two recommendations (p<0.05).Based on criteria, protein energy wasting was detected in 29.1% of the patients.While the nkf kdoqi-2020 daily energy intake recommendation was not consistent with other guideline recommendations, it seems highly compatible with protein energy wasting recommendations such as albumin, body mass index, mid-upper arm circumference, energy, and protein intake.While nkf kdoqi-2020 daily protein intake recommendation complies with european best practice guidelines-2007 recommendations, the level of agreement with protein energy wasting criteria is very low.Conclusion inadequate protein intake is still an ongoing problem in hemodialysis patients.Nkf kdoqi-2020 guidelines provide a more suitable and applicable daily energy intake recommendation for patients compared to the previous guidelines.

Journal: Nephrologie & therapeutique (2022)
Publication types: Journal Article
The Dietary Branched-Chain Amino Acids Transition and Risk of Type 2 Diabetes Among Chinese Adults From 1997 to 2015: Based on Seven Cross-Sectional Studies and a Prospective Cohort Study.

The situation is grim for the prevention and control of type 2 diabetes (t2d) and prediabetes in china.Serum and dietary branched-chain amino acids (bcaas) were risk factors for t2d.However, there is a lack of information on trends in consumption of bcaas and the risk of t2d associated with bcaas intake, based on nationally representative data in china.Thus, we aimed to comprehensively describe the dietary bcaas transition and risk of t2d, at a national level among chinese adults from 1997 to 2015.Methods the data sources were the china health and nutrition survey (chns) and china nutrition and health survey (cnhs).Cross-sectional data on intake were obtained from chns (1997,n= 9,404), chns (2000,n= 10,291), chns (2004,n= 9,682), chns (2006,n= 9,553), chns (2009,n= 9,811), chns (2011,n= 12,686) and cnhs (2015,n= 71,695).Prospective cohort data were obtained chns (1997-2015,n= 15,508).Results from 1997 to 2015, there was a significant decreasing trend in the bcaas intake of chinese adults in all subgroups (p< 0.0001) except for leu in 80 or older, and a decreasing trend in the consumption of bcaas after 40 years old (p< 0.05).The mean intake of bcaas in the population of cohort study was 11.83 ± 3.77g/day.The 95% ci was above the hr of 1.0, when the consumptions were higher than 14.01, 3.75, 6.07, 4.21 g/day in bcaas, ile, leu and val, based on rcs curves.According to the cox proportional hazards models, compared with individuals with bcaas consumption of 10.65-12.37 g/day, the multivariable-adjusted hr for diabetes was 2.26 (95% ci 1.45 to 3.51) for individuals with consumption of bcaas more than 18.52 g/day.A statistically significant positive association between bcaas intake and risk of t2d was observed in males or participants aged 45 years and older, but not in females or participants younger than 45 years.Conclusion our results reveal a trend toward decreased bcaas intake in chinese from 1997 to 2015.After 40 years of age, consumption of bcaas declined with increasing age.Higher bcaas intake was associated with higher risk of t2d.This relationship is more stable among men and middle-aged and elderly people.

Journal: Frontiers in nutrition (2022)
Publication types: Journal Article
Evaluating the long-term care insurance policy from medical expenses and health security equity perspective: evidence from China.

Since the national long-term care (ltci) policy pilot in 2016 of china, the ltci policy has had significant impact on the residents in the pilot area.Methods from the perspective of medical expenses and health security equity, this study selects tracking survey data from the charls database in 2013, 2015, and 2018 and empirically investigates the effect of ltci policy pilot by using differences-in-differences method (did).Moreover, this study measures the economic distribution and health equity of the treated and untreated groups using the concentration and theil indices.Results the results showed that group heterogeneity of medical expenses and health level of elderly in the treatment group were narrowing.Moreover, the policy results showed that the ltci policy pilot significantly affects the outpatient, hospital expenses, and length of stay of elders.Residence registration, income level, and basic medical insurance play a significant regulatory role.Additionally, ltci policy pilot significantly improved the overall health of the elderly.Conclusions the measurement results of inequality show that the policy increases the income of low-income people, lowers the inequality level of outpatient and inpatient reimbursement, and reduces the concentration index of adl disability and serious diseases.However, the inequality of serious diseases is becoming higher.Based on this, this paper provides several suggestions on optimizing the pilot policy of ltci.

Journal: Archives of public health = Archives belges de sante publique (2022)
Publication types: Journal Article
Strategic Inventory Management of Deteriorating Products with Demand Disruptions.

The retailer's strategic inventory management can convince the supplier to present lower wholesale prices in later orders.In practice, there are always unavoidable factors that cause disruption in the supply chain with undeniable impacts on strategic inventories, such as covid-19.This is of greater significance in regard to goods that lose their freshness by time.The impacts of disruption in such products on managed strategic inventories and the retailer's and supplier's consequent behavior have not been addressed in the literature.Therefore, this research investigated the impacts of disruption in demand for deteriorating items on strategic inventories managed by the retailer.The results demonstrated that strategic inventory management is not always a threat for the supplier, and it can even be useful in certain conditions.Changes in goods deterioration levels were found to affect profit more seriously if disruption increased demand in the first period.We also specified the consequences of a decision to or not to manage strategic inventories in different cases of disruption and inventory management cost.

Journal: Arabian journal for science and engineering (2022)
Publication types: Journal Article
Elongation and Contraction of Scallop Sarcoplasmic Reticulum (SR): ATP Stabilizes Ca2+-ATPase Crystalline Array Elongation of SR Vesicles.

The ca2+-atpase is an integral transmembrane ca2+pump of the sarcoplasmic reticulum (sr).Crystallization of the cytoplasmic surface atpase molecules of isolated scallop sr vesicles was studied at various calcium concentrations by negative stain electron microscopy.In the absence of atp, round sr vesicles displaying an assembly of small crystalline patches of atpase molecules were observed at 18 μm [ca2+].These partly transformed into tightly elongated vesicles containing atpase crystalline arrays at low [ca2+] (≤1.3 μm).The arrays were classified as ''tetramer'', "two-rail" (like a railroad) and ''monomer''.Their crystallinity was low, and they were unstable.In the presence of atp (5 m m) at a low [ca2+] of ~0.002 μm, "two-rail" arrays of high crystallinity appeared more frequently in the tightly elongated vesicles and the distinct tetramer arrays disappeared.During prolonged (~2.5 h) incubation, atp was consumed and tetramer arrays reappeared.A specific atpase inhibitor, thapsigargin, prevented both crystal formation and vesicle elongation in the presence of atp.Together with the second part of this study, these data suggest that the atpase forms tetramer units and longer tetramer crystalline arrays to elongate sr vesicles, and that the arrays transform into more stable "two-rail" forms in the presence of atp at low [ca2+].

Journal: International journal of molecular sciences (2022)
Publication types: Journal Article
An icaritin-loaded microemulsion based on coix oil for improved pharmacokinetics and enhanced antitumor efficacy.

Combinational icaritin (ic) and coix seed oil (cso) holds promising potential in the treatment of hepatocellular carcinoma.However, traditional cocktail therapy is facing difficulties to optimize the synergistic antitumor efficacy due to the asynchronous pharmacokinetics.Therefore, we developed an icaritin-loaded microemulsion based on coix seed oil (ic-mes) for improved pharmacokinetics and enhanced antitumor efficacy.The preparation technology of ic-mes was optimized by the box-behnken design and the pharmaceutical properties were characterized in detail.Ic-mes show synergistic antiproliferation against hep g2 cells compared with monotherapy.The mechanism is associated with stronger apoptosis induction via enhancing caspases-3 activity.Ic-mes significantly improve the bioavailability of ic due to the encapsulation of coix oil-based microemulsion and also obtain the desired liver accumulation and elimination.More importantly, ic-mes exhibit the overwhelming antitumor ability among all of the treatments on the hep g2 xenograft-bearing mice.This study verifies the feasibility of using coix oil-based microemulsion to improve the antitumor effect of water-insoluble components.

Journal: Drug delivery (2022)
Publication types: Journal Article