syedzayyan/paper_mill_benchmark huggingface dataset:
Baculovirus Transfer Vectors.

The production of a recombinant baculovirus expression vector normally involves mixing infectious virus dna with a plasmid-based transfer vector and then co-transfecting insect cells to initiate virus infection.The aim of this chapter is to provide an update on the range of baculovirus transfer vectors currently available.Some of the original transfer vectors developed are now difficult to obtain but generally have been replaced by superior reagents.We focus on those that are available commercially and should be easy to locate.These vectors permit the insertion of single or multiple genes for expression, or the production of proteins with specific peptide tags that aid subsequent protein purification.Others have signal peptide coding regions permitting protein secretion or plasma membrane localization.A table listing the transfer vectors also includes information on the parental virus that should be used with each one.Methods are described for the direct insertion of a recombinant gene into the virus genome without the requirement for a transfer vector.The information provided should enable new users of the system to choose those reagents most suitable for their purposes.

Journal: Methods in molecular biology (Clifton, N.J.) (2016)
Publication types: Journal Article;Review
Associations between red blood cell indices and iron status and neurocognitive function in young adults: Evidence from memory and executive function tests and event-related potentials.

Cognitive impairment has been associated with anemia and iron deficiency; however, brain electrophysiological studies correlating red blood cell (rbc) indices and iron status to cognition in adulthood are scarce.We aimed to assess neurocognitive function in young adults of the general population and its correlation with rbc indices and iron status.Neurocognitive function was investigated using scalp-recorded event-related potentials (erps) within the context of a task-switching paradigm.Erps and test performance were also compared across groups of "high"/"low" rbc and iron indices.Working memory was examined using the digit span test, in which mean corpuscular hemoglobin (mch), mean corpuscular volume (mcv), and ferritin were found to be significant predictors of test performance, with higher mch/mcv/ferritin being associated with better test scores.In the switching task, mch, mcv, and ferritin were found to be significant predictors of task performance, with higher mch/mcv/ferritin levels associated with a lower percentage of errors.Electrophysiological results showed that mch and mcv were significant predictors of erps amplitude, with lower mch/mcv levels associated with greater amplitude, which may reflect compensatory processes.P1, n1, p2, and p3 were greater for the low mch/mcv groups.This is the first evidence of association between levels of mch/mcv and brain function while engaged in an executive function task; possibly reflecting brain iron availability.

Journal: Annals of the New York Academy of Sciences (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Integrated Health and Social Care in England: Ten Years On.

Introduction as part of major policy reforms begun in 2010, england introduced a wave of initiatives to encourage more integrated care between health and social care.These built on previous attempts which sought to achieve similar objectives through a focus on better partnership working.This article provides an overview and critical commentary on integrated care policy in england from 2010-2020 based on reviews by regulators, parliamentary committees and the national audit office.Overview of policy integrated care became a priority through the work of the future forum, a group of leading stakeholders established due to concerns about greater competition in public health care.This led to a public statement of shared commitment to integrated care by national health and social care bodies.Early mechanisms included a pooled fund to achieve nationally set objectives, the creation of local authority led partnership boards, and high profile innovation programmes.Later in the 2010's, new health led partnerships became more dominant vehicles to achieve integrated care at regional level.Impact of policy despite progress within a few local areas, and reduction in delayed discharges from hospital the overall picture from national reviews was that expected improvements were not achieved.Emergency admissions to hospital continued to grow, patients within primary care reported being less involved in their care, and health inequalities worsened.The initial response to covid-19 was health-centric contributing to outbreaks in care homes and inadequate supplies of protective personal equipment.The ability of leaders to look beyond their organisations' interests was reported as vital for local progress.National government performance frameworks discouraged system based working and chronic underfunding of social care led to major capacity and workforce challenges.Conclusion the experience of england suggests that greatest progress is made when integrated care focusses on tangible issues and when there is a clear understanding of how success will be measured.Even with considerable investment and intent progress should be expected to be slow and difficult.Layering of numerous policy initiatives provides confusion and can distract from the important work of relationship building.And ultimately, integrated care cannot by itself address major inadequacies in the underlying resources and structural inequalities.

Journal: International journal of integrated care (2021)
Publication types: Journal Article
Copper release from copper nanoparticles in the presence of natural organic matter.

Copper nanoparticles (cu nps) are widely used and inevitably released into aqueous environments, causing ecological and health risks.Ubiquitous natural organic matter (nom) might affect the copper release behaviors from cu nps and their toxicity.This work aims to elucidate how nom affects copper release from cu nps, with a focus on the impacts of nom properties and the nom-cu nps interaction mechanism.The copper release kinetics and different copper fractions induced by representative noms were characterized.The presence of nom led to a more dispersive state of cu nps clusters.Copper release mainly resulted from complexation reactions between cu nps and functional groups of nom.Humic substances were more effective in releasing copper than sodium alginate and bovine serum albumin, due to a higher amount of functional groups and lower molecular weight, which facilitated the contact and complexion reactions.Chlorination treatment of nom significantly decelerated copper release due to the destruction of functional groups and less attachment of nom.However, the copper releasing ability of humic acid was not substantially affected by ca2+-induced coagulation.This study provides better understanding about the persistence and transformation of cu nps in aquatic environments.

Journal: Water research (2015)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Latino-to-Latino: Promotores' Beliefs on Engaging Latino Participants in Autism Research.

In latino(a) communities, promotores de salud (i.e., community health workers; promotores) are becoming critical participants in prevention, health promotion, and the delivery of health care.Although involving culturally diverse participants in research is a national priority, recruitment and retention of research participants from these groups is challenging.Therefore, there is an increased need to identify strategies for successful recruitment of participants from underrepresented minority backgrounds.Our overall study purpose was to gain promotores' perspectives on recruiting latino(a) immigrant community members for an intervention study on autism spectrum disorders (asd).The goal of this paper is to explore insider promotores' views on the barriers and facilitators to research participation in the latino(a) community and learn strategies for recruiting latino(a) participants in a nontraditional destination city.Methods we conducted qualitative focus groups with an established group of promotores known as latinos unidos por la salud (lu-salud), who were members of a community-academic research team.Fifteen lu-salud promotores participated in the focus groups.Focus group interviews were analyzed by using leininger's data analysis enabler.These results will inform our partnerships with promotores and latino(a) neighborhood agencies to increase recruitment for community-based research on promoting awareness of asd among latino(a) families.Results promotores were credible community members able to gain community trust and committed to improving the health and well-being of their latino(a) community, including involving them in research.Latino(a) research involvement meant facilitating community members' engagement to overcome barriers of distrust around legal and health care systems.Challenges included legal uncertainties, language and literacy barriers, health knowledge, and economic hardship.Promotores also voiced the diversity of cultural practices (subcultures) within the latino(a) culture that influenced: (1) research engagement, (2) guidance from promotores, (3) immersion in the latino(a) community, and (4) health and well-being.Experienced promotores, who are living in a nontraditional migration area, believe the primary facilitator to increasing research involvement is latino(a)-to-latino(a) recruitment.Conclusions these findings will aid in building partnerships to recruit participants for future studies that promote early recognition of asd in the latino(a) community.

Journal: Journal of racial and ethnic health disparities (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Hypoxia-induced ebv-circLMP2A promotes angiogenesis in EBV-associated gastric carcinoma through the KHSRP/VHL/HIF1α/VEGFA pathway.

Ebv-encoded circular rna lmp2a (ebv-circ lmp2a) was found to be expressed in ebv-associated gastric carcinoma (ebva gc) and associated with distant metastasis and poor prognosis.Angiogenesis is a key step in tumor invasion and metastasis and plays a crucial role in tumor progression.However, it is unclear whether and how ebv-circ lmp2a is involved in angiogenesis.In this study, we showed that mvd, hif1α, and vegfa expression was increased in ebva gc mouse xenografts with high expression of ebv-circ lmp2a.The expression of ebv-circ lmp2a was positively correlated with mvd, hif1α, and vegfa expression in clinical samples of ebva gc.Knockdown of ebv-circ lmp2a repressed tube formation and migration of huvecs and decreased vegfa and hif1α expression in cancer cells under hypoxia, while ectopic expression of ebv-circ lmp2a reversed these effects.Additionally, knockdown of hif1α blocked the upregulation of ebv-circ lmp2a by hypoxia, and ebv-circ lmp2a interacted with khsrp to enhance khsrp-mediated decay of vhl m rna, leading to the accumulation of hif1α under hypoxia.There was a positive feedback loop between hif1α and ebv-circ lmp2a that promotes angiogenesis under hypoxia.Ebv-circ lmp2a was essential in regulating tumor angiogenesis in ebva gc and might provide a valuable therapeutic target for ebva gc.

Journal: Cancer letters (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Phonetic discrimination, phonological awareness, and pre-literacy skills in Spanish-English dual language preschoolers.

The current study explores variation in phonemic representation among spanish-english dual language learners (dlls, n = 60) who were dominant in english or in spanish.Children were given a phonetic discrimination task with speech sounds that: 1) occur in english and spanish, 2) are exclusive to english, and 3) are exclusive to russian, during fall (age m = 57 months) and spring (age m = 62 months, n = 42).In fall, english-dominant dlls discriminated more accurately than spanish-dominant dlls between english-spanish phones and english-exclusive phones.Both groups discriminated russian phones at or close to chance.In spring, however, groups no longer differed in discriminating english-exclusive phones and both groups discriminated russian phones above chance.Additionally, joint english-spanish and english-exclusive phonetic discrimination predicted children's phonological awareness in both groups.Results demonstrate plasticity in early childhood through diverse language exposure and suggest that phonemic representation begins to emerge driven by lexical restructuring.

Journal: Journal of child language (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Imaging for Temporary Mechanical Circulatory Support Devices.

Heart failure is an important cause of mortality and morbidity in the world.Changes in organ allocation for solid thoracic (lung and heart) transplantation has increased the number of patients on mechanical circulatory support.Temporary mechanical support devices include devices tht support the circulation directly or indirectly such as extracorporeal membrane oxygenation (ecmo) and temporary support for right-sided failure, left-sided failure or biventricular failure.Most often, these devices are placed percutaneously and require either guidance with echocardiography, continuous radiography (fluoroscopy) or both.Furthermore, these devices need imaging in the intensive care unit to confirm continued accurate placement.This review contains the imaging views and nuances of the temporary assist devices (including ecmo) at the time of placement and the complications that can be associated with each individual device.

Journal: Journal of cardiothoracic and vascular anesthesia (2022)
Publication types: Journal Article;Review
Experimental Examination of Solubility and Lipophilicity as Pharmaceutically Relevant Points of Novel Bioactive Hybrid Compounds.

The important physicochemical properties of three novel bioactive hybrid compounds with different groups (-ch3, -f and -cl) were studied, including kinetic and thermodynamic solubility in pharmaceutically relevant solvents (buffer solutions and 1-octanol) as well as partition coefficient in system 1-octanol/buffer p h 7.4.The aqueous solubility of these chemicals is poor and ranged from 0.67 × 10-4to 1.98 × 10-3mol·l-1.The compounds studied are more soluble in the buffer p h 2.0, simulating the gastrointestinal tract environment (by an order of magnitude) than in the buffer p h 7.4 modelling plasma of blood.The solubility in 1-octanol is significantly higher; that is because of the specific interactions of the compounds with the solvent.The prediction solubility behaviour of the hybrid compounds using hansen's three-parameter approach showed acceptable results.The experimental solubility of potential drugs was successfully correlated by means of two commonly known equations: modified apelblat and van't hoff.The temperature dependencies of partition coefficients of new hybrids in the model system 1-octanol/buffer p h 7.4 as a surrogate lipophilicity were measured by the shake flask method.It was found that compounds demonstrated a lipophilic nature and have optimal values of partition coefficients for oral absorption.Bioactive assay manifested that prepared compounds showed antifungal activities equal to or greater than fluconazole.In addition, the thermodynamic aspects of dissolution and partition processes have been examined.Bioactive assay manifested that prepared compounds showed antifungal activities equal to or greater than the reference drug.

Journal: Molecules (Basel, Switzerland) (2022)
Publication types: Journal Article
The Use of Guided Reflection in Simulation-Based Education With Prelicensure Nursing Students: An Integrative Review.

This integrative review synthesized the findings of studies to determine the uses and outcomes of guided reflection in simulation-based education (sbe) with prelicensure nursing students.Method a search of cumulative index to nursing and allied health (cinahl) plus, education resources information center (eric), education research complete (erc), and psych info using the key words reflection, guided reflection, simulation (nursing education or nursing student), traditional, and baccalaureate identified 18 articles.Results findings suggest facilitation of guided reflection in sbe has a positive effect on leadership development, clinical judgment, student satisfaction, and critical thought.Development of a tool to accurately measure guided reflection is warranted.Conclusion current evidence on the use of guided reflection in sbe calls for a major paradigm shift in higher education.Guided sbe experiences enhance participant knowledge, and guided reflection should be used as an innovative teaching strategy in sbe.[j nurs educ.2022;61(2):73-79.].

Journal: The Journal of nursing education (2022)
Publication types: Journal Article;Review
Pedicle subtraction osteotomy in the thoracic spine and thoracolumbar junction: a retrospective series of 28 cases.

Pedicle subtraction osteotomy is a well-described surgical technique for treatment of kyphotic deformity in the spine.It is not widely used for treatment of thoracic kyphosis.We present the first documented series of 28 patients who underwent this procedure in 3 international centers.These patients presented with severe deformity with a wide range of aetiologies.Indications kyphosis larger than 70 degrees, which is demonstrably rigid based on dynamic imaging.Materials and methods 28 patients underwent surgery following pre-op neurological and radiographic assessment to fully assess the deformity.A triangular osteotomy was carried out using intraoperative navigation techniques.The patients were assessed post-operatively again with clinical and radiographic parameters at regular follow-up.Results the mean odi score after surgery was 24.7 (16-42) while the pre-op was 53.4 (38-76).Mean thoracic kyphosis was improved from 64.2° (±20.1°) to 41.1° (±17.4°) resulting in a mean sagittal correction of 23.1°.Mean segmental correction at the pso for all 28 cases was 17.8° (±8.1°).Stratified by region we found different values for the pso correction: between t1 and t5 (6 cases) it was 17.5° (±5.4°) and between t6 and t9 (4 cases) 18.2° (±4.7°) and between t10 and l1 (18 cases) 26.2° (±5.2°).Fbi index was 22.3° pre-op and improved to 7.8° post-op.Calculations were performed with microsoft excel (2011 microsoft, redmond, wa).Conclusions global sagittal balance was statistically improved in this series as demonstrated by fbi and c7 sva correction.

Journal: European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society (2015)
Publication types: Journal Article;Multicenter Study
WDR73 Depletion Destabilizes PIP4K2C Activity and Impairs Focal Adhesion Formation in Galloway-Mowat Syndrome.

(1) background: galloway-mowat syndrome (gamos) is a rare genetic disease, classically characterized by a combination of various neurological symptoms and nephrotic syndrome.Wdr73 is the pathogenic gene responsible for gamos1.However, the pathological and molecular mechanisms of gamos1, especially nephrotic syndrome caused by wdr73 deficiency, remain unknown.(2) methods and results: in this study, we first observed remarkable cellular morphological changes including impaired cell adhesion, decreased pseudopodia, and g2/m phase arrest in wdr73 knockout (ko) hek 293 cells.The differentially expressed genes in wdr73 ko cells were enriched in the focal adhesion (fa) pathway.Additionally, pip4k2c, a phospholipid kinase also involved in the fa pathway, was subsequently validated to interact with wdr73 via protein microarray and gst pulldown.Wdr73 regulates pip4k2c protein stability through the autophagy-lysosomal pathway.The stability of pip4k2c was significantly disrupted by wdr73 ko, leading to a remarkable reduction in pip2 and thus weakening the fa formation.In addition, we found that podocyte-specific conditional knockout (wdr73 cko) mice showed high levels of albuminuria and podocyte foot process injury in the adr-induced model.Fa formation was impaired in primary podocytes derived from wdr73 cko mice.(3) conclusions: since fa has been well known for its critical roles in maintaining podocyte structures and function, our study indicated that nephrotic syndrome in gamos1 is associated with disruption of fa caused by wdr73 deficiency.

Journal: Biology (2022)
Publication types: Journal Article
Anti-CTLA-4 and anti-PD-1 immunotherapies repress tumor progression in preclinical breast and colon model with independent regulatory T cells response.

The recent development of immunotherapy represents a significant breakthrough in cancer therapy.Several immunotherapies provide robust efficacy gains in a wide variety of cancers.However, in some patients the immune checkpoint blockade remains ineffective due to poor therapeutic response and tumor relapse.An improved understanding of the mechanisms underlying tumor-immune system interactions can improve clinical management of cancer.Here, we report preclinical data evaluating two murine antibodies corresponding to recent fda-approved antibodies for human therapy, e.g.Anti-ctla-4 and anti-pd-1.We demonstrated in two mouse syngeneic grafting models of triple negative breast or colon cancer that the two antibodies displayed an efficient anticancer activity, which is enhanced by combination treatment in the breast cancer model.We also demonstrated that ctla-4 targeting reduced metastasis formation in the colon cancer metastasis model.In addition, using cytometry-based multiplex analysis, we showed that anti-ctla-4 and anti-pd-1 affected the tumor immune microenvironment differently and in particular the tumor immune infiltration.This work demonstrated anti-cancer effect of ctla-4 or pd-1 blockade on mouse colon and triple negative breast and on tumor-infiltrating immune cell subpopulations that could improve our knowledge and benefit the breast and colon cancer tumor research community.

Journal: Translational oncology (2022)
Publication types: Journal Article