syedzayyan/paper_mill_benchmark huggingface dataset:
Inhibition of miR-182-5p attenuates ROS and protects against myocardial ischemia-reperfusion injury by targeting STK17A.

Reperfusion therapy for acute myocardial infarction inevitably leads to ischemia-reperfusion (i/r) injury.A number of mi rnas are reported to be involved in i/r injury.This study aims to investigate the role and underlying mechanism of mi r-182-5p in i/r injury.Anin vivomodel of i/r-induced rat myocardial injury and anin vitromodel of h/r h9c2 cells were established to investigate the role and mechanism of mi r-182-5p in i/r injury.The myocardial infarct size was determined by ttc staining.The serum ck-mb level was determined by elisa kit.The mi r-182-5p inhibitors or mimics were used to down-regulate or up-regulate its expression.The apoptosis and ros were detected by flow cytometry.The expression of the proteins was detected by western blot.The binding of stk17a and mi r-182-5p was validated by dual-luciferase reporter assay.The mi r-182-5p was confirmed to be highly expressed in i/r injury rats and h/r h9c2 cells.Inhibition of mi r-182-5p significantly reduced the infarct size and decreased the serum ck-mb level of i/r rats, and significantly reduced the ros level but increased the level of mn sod and catalase.While, an opposite effect was observed in the mi r-182-5p mimics group.Furthermore, our results suggested that mi r-182-5p targeted stk17a, and tk17a knockdown significantly increased the apoptotic rate and ros level.The inhibitory effect of mi r-182-5p inhibitors on apoptotic rate, ros, mn sod, and catalase levels were abrogated by si stk17a.These results indicate that mi r-182-5p regulates the apoptosis and ros and protects against myocardial i/r injury by targeting stk17a.

Journal: Cell cycle (Georgetown, Tex.) (2022)
Publication types: Journal Article
Secondary Central Nervous System Lymphoma: Updates in Treatment and Prophylaxis Strategies.

Referring to any central nervous system (cns) involvement with preceding or concurrent systemic disease, secondary cns lymphoma (scnsl) lacks a clear standard of care and historically carries a very poor prognosis.Aggressive histologies predominate, most notably diffuse large b cell lymphoma (dlbcl), with higher relative frequency in burkitt lymphoma but lower absolute incidence.Therapeutic strategies commonly feature intensive cns-penetrant chemotherapy, including methotrexate, cytarabine, and others.Combination regimens, novel targeted agents, and cellular therapy considerations are reviewed, noting that patients with scnsl are often excluded from clinical trials and dedicated scnsl studies are historically limited.Given these challenges, there has been renewed attention on cns prophylaxis as well as strategies for early cns detection.Prophylaxis is standard of care in burkitt lymphoma, whereas its role in dlbcl and related histologies is increasingly unclear.

Journal: Current treatment options in oncology (2022)
Publication types: Journal Article;Review
Evaluating novel methods of outcome assessment following cardiac arrest.

Introduction we compared novel methods of long-term follow-up after resuscitation from cardiac arrest to a query of the national death index (ndi).We hypothesized use of the electronic health record (ehr), and internet-based sources would have high sensitivity for identifying decedents identified by the ndi.Methods we performed a retrospective study including patients treated after cardiac arrest at a single academic center from 2010 to 2018.We evaluated two novel methods to ascertain long-term survival and modified rankin scale (m rs): 1) a structured chart review of our health system's ehr; and 2) an internet-based search of: a) local newspapers, b) ancestry.com, c) facebook, d) twitter, e) instagram, and f) google.If a patient was not reported deceased by any source, we considered them to be alive.We compared results of these novel methods to the ndi to calculate sensitivity.We queried the ndi for 200 in-hospital decedents to evaluate sensitivity against a true criterion standard.Results we included 1,097 patients, 897 (82%) alive at discharge and 200 known decedents (18%).Ndi identified 197/200 (99%) of known decedents.The ehr and local newspapers had highest sensitivity compared to the ndi (87% and 86% sensitivity, respectively).Online sources identified 10 likely decedents not identified by the ndi.Functional status estimated from ehr, and internet sources at follow up agreed in 38% of alive patients.Conclusions novel methods of outcome assessment are an alternative to ndi for determining patients' vital status.These methods are less reliable for estimating functional status.

Journal: Resuscitation (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural
Parental anxiety and form of parenting during the COVID-19 pandemic.

The massive development of information technology based on big data, internet, and artificial intelligence has brought fundamental changes to human patterns and lifestyles, especially after the covid-19 pandemic that hit globally, has added to a large and complex problems in parenting, as well as demanding people to take care of their children.Parents must be able to adapt and reposition themselves with new and effective forms of parenting, this can increase parental anxiety.To determine the level of parental anxiety, this research was conducted using a quantitative descriptive method through the distribution of questionnaires based on the gad-7 instrument.This study focuses on efforts to capture the level of parental anxiety and the need for a new form of parenting.The results can be the basis for further research to find and develop new forms of parenting.The results of research on 669 parents living in west java, indonesia, showed that the level of parental anxiety was 63.08% at the level of moderate and severe anxiety.The level of parental satisfaction regarding the form of parenting used is at a low level of 67.12%, while the level of parental interest in the new form of parenting is at a very high level of 98.51%.The need for the latest form of parenting that can respond to the challenges and demands of the times is very necessary to minimize parental anxiety.

Journal: International journal of child care and education policy (2022)
Publication types: Journal Article
Ultrathin indium vanadate/cadmium selenide-amine step-scheme heterojunction with interfacial chemical bonding for promotion of visible-light-driven carbon dioxide reduction.

The formation of interfacial chemical bonding in heterostructures plays an important role in the transport of carriers.Herein, we firstly prepared ultrathin in vo4nanosheet (ns) with a thickness of 1.5 nm.Diethylenetriamine-modified cd se (cd se-deta) nanobelts are in-situ deposited on the surface of ultrathin in vo4ns to build a in vo4/cd se-deta step-scheme (s-scheme) heterojunction photocatalysts.The protonated deta acts as an amine-bridge to promote the formation of a tight chemical bond at the interface of in vo4/cd se-deta, thereby promoting the transfer of carriers at the interface.For photocatalytic co2reduction, the rationally designed in vo4/cd se-deta s-scheme photocatalyst exhibits a remarkable co generation rate of 27.9 μmol h-1g-1at 420 nm, which is 3.35 and 3.39 times higher than that of cd se-deta and in vo4ns, respectively.The new method by using interfacial chemical bonding to facilitate interfacial charge transportation provide a promising strategy for improve photocatalysis.

Journal: Journal of colloid and interface science (2022)
Publication types: Journal Article
Mechanisms of carbapenemase-mediated resistance among high-risk Pseudomonas aeruginosa lineages in Peru.

S pseudomonas aeruginosa is one of the leading causes of healthcare-associated infections globally.High-risk carbapenemase-encoding p.Aeruginosa clones are disseminating in many regions.The aim of this study was to learn more about the lineages and mechanisms of resistance of p.Aeruginosa circulating in peru.Methods a total of 141 carbapenemase-producing isolates recovered from hospitalized and ambulatory patients in lima were sequenced and analyzed to infer their lineages through whole-genome sequence typing (wg st) and to identify their antimicrobial resistance genes.Results wg st identified nine sequence types (sts); st111 and st357 were the most frequently encountered (44.0% and 38.3%, respectively), followed by st179 (8.5%), with the remaining six detected only sporadically.Among st357 isolates, 96.3% carried the novel bla imp-93allele, whereas the remainder harbored bla imp-74.74.2% of st111 isolates co-harbored bla imp-18and bla vim-2, while the rest carried either of these genes individually.all other st lineages carried a single carbapenemase, which was either bla imp-16, bla imp-74, or bla vim-2.Conclusion our study shows that the high-risk p.Aeruginosa clones st357, which harbors the novel bla imp-93,and st111, which carries bla imp-18and bla vim-2,have apparently become endemic in the region.

Journal: Journal of global antimicrobial resistance (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
LncRNA RHPN1-AS1 inhibition induces autophagy and apoptosis in prostate cancer cells via the miR-7-5p/EGFR/PI3K/AKT/mTOR signaling pathway.

Lnc rna rhpn1-as1 (rhpn1-as1) has been confirmed to promote tumor progression in multiple cancers and is upregulated in prostate cancer (pca), but whether it has an effect on pca progression remains unclear.In this study, we found that pca patients with high rhpn1-as1 expression had a shorter survival time, and rhpn1-as1 was significantly upregulated in pca tissues and cells.Based on informatics analysis we predicted that mi r-7-5p binds to 3'utr of rhpn1-as1 and epidermal growth factor receptor (egfr) and verified it by luciferase reporter gene assay.Subsequently, we transfected pca cells with rhpn1-as1 overexpression vector (rhpn1-as1), knockdown plasmids (sh-rhpn1-as1) and/or mi r-7-5p mimics or inhibitor and/or overexpression vector (egfr) or small interfering rna of egfr (si-egfr) or its control, and found that overexpression of rhpn1-as1 inhibited mi r-7-5p expression and promoted egfr expression, silencing rhpn1-as1 inhibited proliferation and invasion, and induced g2/m arrest, apoptosis and autophagy in pca cells.3ma (an inhibitor of autophagy)-mediated autophagy inhibition attenuated rhpn1-as1 inhibition-induced apoptosis.Overexpression mi r-7-5p or silencing egfr promoted lc3-i to lc3-ii conversion, enhanced autophagy activity, induced cleaved-caspase-3 expression and apoptosis in pca cells.Furthermore, overexpression of rhpn1-as1 promoted phosphorylation of phosphatidylinositol 3-kinase (pi3k), akt and m tor, inhibited lc3-i to lc3-ii conversion and reduced apoptosis in pca cells, while gsk2126458 (an inhibitor of pi3k) reversed the effect of rhpn1-as1 on pca cells.In summary, rhpn1-as1 acted as a ce rna of mi r-7-5p to upregulate egfr expression, silencing rhpn1-as1 suppressed pca tumor progression by inducing autophagy and apoptosis in pca cells through the mi r-7-5p/egfr/pi3k/akt/m tor pathway.

Journal: Environmental toxicology (2022)
Publication types: Journal Article
The outlook of doctors toward telemedicine: A cross-sectional study of knowledge, awareness, and attitude in central India.

In an underdeveloped country like india where there are varied constraints in accessing healthcare, telemedicine can prove to be instrumental in providing access to the scant medical resources and infrastructures.The irony lies in its underutilization, which is multifactorial.Objective the present research was planned to evaluate the level of awareness, knowledge, and attitude toward telemedicine among the faculty members of tertiary-care teaching centers in chhattisgarh, india.Methods a questionnaire-based survey was conducted to collect appropriate data, which were utilized for analysis as well as various intergroup analyses (among different designations, age groups, sex, and worksite).The data have been presented as median and percentage, while for intergroup comparison mann-whitney test was performed.Results among the 115 respondents, only 34% were females.The mean age was around 40.7 years, and 58% were <40 years of age.Their median scores of awareness, knowledge, and attitude were estimated to be 06, 14, and +9, respectively.No statistically significant difference was observed in the awareness, knowledge, and attitude among the faculty members belonging to different designations, age groups, and worksites.Conclusions though, in general, the faculty members have demonstrated a favorable attitude toward the use of telemedicine yet most of them scored below the median mark.Furthermore, the estimated level of knowledge and awareness was also mediocre.

Journal: Journal of family medicine and primary care (2021)
Publication types: Journal Article
Low agreement among patients and clinicians about urgency and safety to wait for assessment in primary care after hours medical care: results of cross-sectional matched surveys.

Discordance between patient and clinician perceived urgency may drive "inappropriate" presentations to after-hours medical services.This paper investigates the level of agreement between patient and clinicians' perceptions of urgency and safety to wait for an assessment at after-hours primary care services in the act.Methods cross-sectional survey voluntarily completed by patients and then clinicians at after-hours medical services in may/june, 2019.Agreement between patients and clinicians is measured by fleiss kappa.Agreement is presented overall, within specific categories of urgency and safety to wait, and by after-hours service type.Results 888 matched records were available from the dataset.Overall inter-observer agreement between patients and clinicians on the urgency of presentations was slight (fleiss kappa = 0.166; 95% ci 0.117-0.215, p < 0.001).Agreement within specific ratings of urgency ranged from very poor to fair.Overall inter-rater agreement on how long it would be safe to wait for assessment was fair (fleiss kappa = 0.209; 95% ci 0.165-0.253, p < 0.001).Agreement within specific ratings ranged from poor to fair.By site type, agreement between patients and clinicians on urgency ranged from not significant to fair and agreement for safety to wait ranged from very poor to slight.Agreement on urgency of issue was more often reported among patients attending their usual health service or seeing their usual clinician compared to patients attending an unfamiliar health service or clinician (χ2(1) = 7.283, p = 0.007 and χ2(1) = 16.268, p < 0.001, respectively).Conclusions low levels of agreement between patients and clinicians on perceived urgency and safety to wait for issues to be assessed indicate potential inefficiency in primary care use after-hours.Agreement on urgency of issues was more common among patients attending a familiar health service or familiar clinician.Improving health literacy, particularly health system literacy, and supporting continuity of care may help to support patients to engage with the most appropriate level of care at the most appropriate time.

Journal: BMC health services research (2023)
Publication types: Journal Article
Hospitalized with stroke at the weekend: Higher cost and risk of early death?

S previous studies have shown that weekend hospitalizations are associated with poorer health outcomes and higher mortality ("weekend effect").However, few of these studies have adjusted for disease severity and little is known about the effect on costs.This work investigates the weekend effect and its costs for patients with cerebral infarction in germany, adjusting for patient characteristics and proxies of stroke severity.Methods adult patients with a cerebral infarction hospitalization 10th revision of the international statistical classification of diseases and related health problems (icd-10: i63) between 01 january 2014 and 30 june 2017 were included from german health claims (aok plus dataset).Propensity score matching was used to match patients hospitalized on weekends or on public holidays (weekend group) with patients hospitalized during the working week (workday group), based on baseline characteristics and proxies for disease severity such as concomitant diagnoses of aphasia, ataxia, and coma, or peg tube at index hospitalization.Matched cohorts were compared in terms of in-hospital, 7-day, and 30-day mortality, as well as risk and costs of stroke and rehabilitation stays in the year after first stroke.Results of 32,311 patients hospitalized with cerebral infarction between 01 january 2014 and 30 june 2017, 8409 were in the weekend group and 23,902 in the workday group.After propensity score matching, 16,730 patients were included in our study (8365 per group).Matched cohorts did not differ in baseline characteristics or stroke severity.In the weekend group, the risk of in-hospital death (11.2%) and the seven-day mortality rate (6.8%) were 13.1% and 17.2% higher than in the workday group, respectively (bothp< 0.01).The hazard ratio for death in the weekend group was 1.1 (p= 0.043).The risks of subsequent stroke hospitalization and rehabilitation stays for a stroke were 8.4% higher and 5.5% higher in the weekend group (bothp= 0.02).As a result, the stroke-related hospitalization and rehabilitation costs per patient year were, respectively, 5.6% and 8.0% higher in the weekend group (bothp= 0.01).Conclusions a significant weekend effect emerged after controlling for observable patient characteristics and proxies of stroke severity.This effect also resulted in higher costs for patients admitted on weekends.

Journal: International journal of stroke : official journal of the International Stroke Society (2022)
Publication types: Journal Article
Diagnostic performance of single-phase CT angiography in detecting large vessel occlusion in ischemic stroke: A systematic review.

To systematically review the diagnostic performance of single-phase ct angiography (cta) in detecting intracranial large vessel occlusion (lvo).Method medline and embase were searched for studies investigating the diagnostic performance of single-phase cta in detecting lvo.Study quality was assessed.Sensitivity and specificity were calculated and meta-analyzed with a bivariate random-effects model.Heterogeneity was assessed with a chi-squared test.Results eleven studies were included.High risk of bias with regard to "patient selection", "reference standard", and "flow and timing" was present in 4, 1, and 2 studies, respectively.In 7 studies, it was unclear whether reference tests were interpreted blinded to cta readings.There was variability in types of vessel segments analyzed, resulting in heterogeneous sensitivity and specificity (p < 0.05).Two studies provided data for the proximal anterior circulation (distal intracranial carotid artery, a1-, a2-, m1- and m2-segments), with pooled sensitivity of 88.4 % (95 % ci: 62.2-97.2 %) and pooled specificity of 98.5 % (95 % ci: 33.2-100 %).One study suggested that multiphase cta improved agreement between nonexperts and an expert in detecting a1-, a2-, m1-, m2-, and m3-segment occlusions compared to single-phase cta (ĸ = 0.72-0.76 vs.ĸ = 0.32-0.45).No other included study reported added value of advanced cta (ct perfusion, 4d-cta, or multiphase cta) compared to single-phase cta in detecting proximal anterior circulation lvo.Conclusion there is lack of high-quality studies on the diagnostic performance of single-phase cta for lvo detection in the proximal anterior circulation.The added value of advanced cta techniques in detecting proximal anterior circulation lvo is not completely clear yet.

Journal: European journal of radiology (2021)
Publication types: Journal Article;Systematic Review
Utilising exhaled nitric oxide information to enhance diagnosis and therapy of respiratory disease - current evidence for clinical practice and proposals to improve the methodology.

A non-invasive tool to diagnose respiratory diseases and to follow treatment has long been looked-for.Exhaled nitric oxide (no) is a promising marker of inflammation in asthma but nearly 25-years of research has shown that it works in only certain endotypes of asthma.The modelling of no dynamics of the lung can give more information than a single feno value.Areas covered: the estimation of the no production in the conducting airways and in the gas exchange area has given new insight of the no production in diseases beyond asthma.In this article, we discuss the importance of methodology for no measurement in the exhaled breath and the indication of applying this technique to detect respiratory disorders.This narrative review is an attempt to examine and discuss the physiological basis underlying exhaled no measurements and the clinical evidence of the usefulness of this method in asthma and various other respiratory disorders.Expert commentary: estimation of the no parameters would aid in our understanding of the no dynamics of the lung and thereby give more knowledge how to interpret the measured feno value in clinical practice.

Journal: Expert review of respiratory medicine (2017)
Publication types: Journal Article;Review
Do we need to change empiric antibiotic use following natural disasters? A reflection on the Townsville flood.

Introduction skin and soft tissue infections have the potential to affect every patient admitted to a surgical service.Changes to the microbiota colonizing wounds during natural disasters, such as the townsville floods of 2019, could impact empiric antibiotic choice and need for return to theatre.Methods this retrospective observational cohort study reviews culture data and demographics for patients undergoing surgical debridement of infected wounds over a six-month period starting in november 2018 to may 2019 at the townsville hospital.Results of the 408 patients requiring operative intervention, only 61 patients met the inclusion criteria.The groups were comparative in terms of age and gender, but a greater proportion of patients (40.5% versus 29.1%, p = 0.368) in the post-flood group were diabetic.Common skin commensals, such as staphylococcus aureus, were the most common pathogen in both groups, however the post-flood group had a higher proportion of atypical organisms (14 versus 8 patients), and an increased need for repeated debridement for infection control (24 versus 14 patients).Conclusion wound swabs and tissue culture are imperative during surgical debridement and may guide the use of more broad-spectrum coverage following a significant flooding event.

Journal: ANZ journal of surgery (2022)
Publication types: Journal Article;Observational Study