syedzayyan/paper_mill_benchmark huggingface dataset:
Diabetes Distress in Emerging Adulthood: Content Validity of the Problem Areas in Diabetes-Emerging Adult Version (PAID-EA) Using Qualitative Analysis.

The purpose of this study was to explore the emotional work of diabetes during emerging adulthood and to explicate the validity of a newly developed measure of diabetes distress (dd) for use with emerging adults living with type 1 diabetes mellitus (t1dm), the problem areas in diabetes-emerging adult version (paid-ea).Methods young people ages 18 to 30 with t1dm were recruited online to complete a cross-sectional survey including measures of dd, depressive symptomology, and the paid-ea.To evaluate content validity, 2 open-ended questions asked what was the most significant emotion or worry discussed in the survey items and what feelings were missed in those items.Responses were analyzed using directed qualitative content analysis.Results a total of 254 (87%) participants responded to at least 1 of the 2 open-ended questions.Three themes and 1 subtheme were identified: (1) fear of the future with the subtheme of worry about the cost of diabetes, (2) acute worries about living with diabetes, and (3) challenges with finding support.More paid-ea items corresponded with these themes than items on the original problem areas in diabetes or center for epidemiologic studies depression scale, supporting the validity of the paid-ea and clarifying the developmental-stage-specific aspects of dd.Conclusions emerging adulthood is a period in which the future should hold infinite possibility, but young people with t1dm describe a staggering fear of the future with markedly limited possibilities, supporting the need to measure the developmental-stage-specific experience of dd as captured on the paid-ea.

Journal: The science of diabetes self-management and care (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural
Role of circulating microRNA-21 and microRNA-215 in the diagnosis of hepatitis C related hepatocellular carcinoma.

Introduction several micro ribonucleic acids (mi rnas) are deregulated in hepatocellular carcinoma (hcc).Others are linked to clinical pathological features of hcc.The goal of this study was to investigate whether mi rna-21 and mi rna-215 gene expression could be used as a non-invasive diagnostic tool to diagnose hcc.Methodology the gene expression of mature mi rna -21 and mi rna -215 in serum was analysed retrospectively using singleplex taq man two-step stem-loop quantitative real-time reverse-transcription pcr in 40 patients with hcc, 40 with chronic hepatitis c virus (hcv) with cirrhosis and 40 apparently healthy controls.Results expression of mi rna -21 was significantly more down regulated in patients with hcc than in those with non-cirrhotic hcv (p = 0.007; odds ratio = 5; 95% confidence interval 1.6-15.4).The receiver operating curve analysis of the ability of mi rna-21 expression to discriminate between hcc and non-cirrhotic hcv revealed an area under the curve of 0.712 with 70% sensitivity and 68% specificity at a cut-off of ≤ 1.4468.Thus, the expression level of mi rna -21 could discriminate hcc from non-cirrhotic hcv.Significant positive correlation was observed between expression levels of micro rna-21 and mi rna -215 (r = 0.783, p < 0.001), but no association was observed between expression level of mi r-215 and hcc or chronic hcv (p = 0.474).Conclusions mi rna-21 may be a useful, non-invasive tool for diagnosing hcc.Non-cirrhotic hcv patients have five times the risk of developing hcc when the mi rna -21 level ≤ 1.4468.

Journal: Journal of infection in developing countries (2021)
Publication types: Journal Article
Feasibility of Using Games to Improve Healthy Lifestyle Knowledge in Youth Aged 9-16 Years at Risk for Type 2 Diabetes: Pilot Randomized Controlled Trial.

Mobile games can be effective and motivating tools for promoting children's health.Objective we aimed to determine the comparative use of 2 prototype serious games for health and assess their effects on healthy lifestyle knowledge in youth aged 9-16 years at risk for type 2 diabetes (t2d).Methods a 3-arm parallel pilot randomized controlled trial was undertaken to determine the feasibility and preliminary effectiveness of 2 serious games.Feasibility aspects included recruitment, participant attitudes toward the games, the amount of time the participants played each game at home, and the effects of the games on healthy lifestyle and t2d knowledge.Participants were allocated to play diabetic jumper (n=7), ari and friends (n=8), or a control game (n=8).All participants completed healthy lifestyle and t2d knowledge questionnaires at baseline, immediately after game play, and 4 weeks after game play.Game attitudes and preferences were also assessed.The primary outcome was the use of the game (specifically, the number of minutes played over 4 weeks).Results in terms of feasibility, we were unable to recruit our target of 60 participants.In total, 23 participants were recruited.Participants generally viewed the games positively.There were no statistical differences in healthy lifestyle knowledge or diabetes knowledge over time or across games.Only 1 participant accessed the game for an extended period, playing the game for a total of 33 min over 4 weeks.Conclusions it was not feasible to recruit the target sample for this trial.The 2 prototype serious games were unsuccessful at sustaining long-term game play outside a clinic environment.Based on positive participant attitudes toward the games, it is possible to use these games or similar games as short-term stimuli to engage young people with healthy lifestyle and diabetes knowledge in a clinic setting; however, future research is required to explore this area.Trial registration australia new zealand clinical trials registry actrn12619000380190; review.aspx?id=377123.

Journal: JMIR formative research (2022)
Publication types: Journal Article
Risk factors and a nomogram for frailty in Chinese older patients with Alzheimer's disease: A single-center cross-sectional study.

This study aimed to determine the risk factors of frailty in chinese older patients with alzheimer's disease (ad) and then construct a nomogram for frailty in this population.A total of 205 eligible older ad patients were recruited.Patients' general demographic characteristics were collected through a self-designed questionnaire.A nomogram was constructed for frailty based on the risk factors identified from the multivariate analysis.The discrimination and calibration capabilities of this nomogram were assessed with the c-index and calibration curve, respectively.The results showed that older age, no regular exercise habit, severe cognitive decline, and low social support were identified as important risk factors of frailty in ad patients.The c-index of the nomogram was 0.884 by bootstrapping validation, and the calibration curve of the nomogram showed high coherence between the predicted and actual probabilities of frailty.In conclusion, this nomogram was validated to have favorable discrimination and calibration capabilities.

Journal: Geriatric nursing (New York, N.Y.) (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
To the Future of Ethnogeriatric Research and Publication.

The reinstatement of the journal of the american geriatrics society section on ethnogeriatrics coincides with more rapid growth in older populations that are classified as ethnic and racial minorities in the united states than those classified as non-hispanic white.By 2060, 40% of older americans are predicted to belong to a minority.Important needs for ethnogeriatric research and publication include: making sure research populations are meaningful and precise rather than using categories that include many unrelated groups; and increasing research among smaller ethnic populations of older adults.Topics in need of attention include epidemiology of common geriatric illness among the smaller populations, and social determinants for those in which disparities have been established; the extent and effectiveness of use of culturally and linguistically appropriate services standards in geriatric care; examination of communication strategies used in provider/patient interaction, especially use of interpreters; ethnic differences in treatment of older patients; and models of use of community health workers from older patients' own cultural communities.Stresses related to caregiving in cultures emphasizing the importance of family care are important to understand acceptable models of long-term care for diverse families.The new ethnogeriatric section opens the opportunity for geriatric researchers, especially those from diverse backgrounds, to explore important issues in cross-cultural geriatric care; their findings can then form the basis of expanded ethnogeriatric curriculum for training future providers for the growing population of diverse older americans.

Journal: Journal of the American Geriatrics Society (2019)
Publication types: Journal Article
MiR-124 Regulates IQGAP1 and Participates in the Relationship Between Morphine Dependence Susceptibility and Cognition.

Long-term excessive use of morphine leads to addictive diseases and affects cognitive function.Cognitive performance is associated with genetic characteristics.mi r-124 plays a critical regulatory role in neurogenesis, synaptic development, brain plasticity, and the use of addictive substances.As a scaffold protein, iqgap1 affects learning and memory dose-dependent.However, the role of mi r-124 and its target protein as potential addiction biomarkers and the impact on cognitive function have not been fully explored.Method a total of 40 patients with morphine dependence and 40 cases of healthy people were recruited.We collected basic and clinical information about the two groups.The generalized anxiety disorder scale (gad-7), patient health questionnaire-9(phq-9), montreal cognition assessment scale (mo ca), pittsburgh sleep quality index (psqi) were used to assess the severity of depression, anxiety, depressive symptoms, cognitive dysfunction, and sleep quality.Results compared to the control group, the morphine-dependent group had higher gad-7, phq-9, psqi scores, and more elevated mi r-124 levels but lower moca scores and iqgap1 levels.Mi r-124, iqgap1, the average intake last year were related to oasi scores.mi r-124, iqgap1, phq-9 were associated with moca scores.In the multiple regression model, the levels of mi r-124 and iqgap1 were independent factors influencing the severity of morphine dependence.The level of mi r-124 was an independent factor influencing the severity of cognitive impairment in patients with morphine dependence.In addition, the luciferase report confirmed that iqgap1 m rna is the direct target of mi r-124.Conclusion mi r-124 and its target protein iqgap1 are involved in the regulation of addiction and cognitive function in patients with morphine dependence.

Journal: Frontiers in psychiatry (2022)
Publication types: Journal Article
Development of a rapid startup method of direct electron transfer-dominant methanogenic microbial electrosynthesis.

The rapid startup of carbon dioxide reduction-methanogenic microbial electrosynthesis is crucial for its industrial application, and the development of cathode biofilm is the key to its industrialization.Based on the new discovery that biofilm formed by placing graphite felt in an anaerobic reactor was electroactive, with strong direct electron transfer and methanogenesis ability (24.52 m l/l/d), a new startup method was developed.The startup time was shortened by at least 20 days and charge transfer resistance was reduced by 4.45-10.78 times than common startup methods (inoculating cathode effluent or granular sludge into the cathode chamber).The new method enriched electroactive bacteria.Methanobacterium and methanosaeta accounted for 62.04% and 34.96%, respectively.The common methods inoculating cathode effluent or granular sludge enriched hydrogenotrophic microorganisms (>95%) or methanosaeta (54.10%) due to the local environments of cathode.This new rapid and easy startup method may support the scale-up of microbial electrosynthesis.

Journal: Bioresource technology (2022)
Publication types: Journal Article
Pelvic Organ Prolapse Takes Up Space in the Bedroom: A Mixed Method Exploration of the Circular Sexual Response Cycle in Women With Pelvic Organ Prolapse.

Pelvic organ prolapse (pop) is a multidimensional reproductive health issue, which negatively affects women's sexual well-being.Using the circular sexual response cycle as a framework, we sought to evaluate women's sexual experiences living with pop.Methods measures of genital self-image, sexual distress, sexual satisfaction, and sexual function were administered before conducting semistructured interviews.Participants were 16 heterosexual partnered women seeking surgical and nonsurgical treatment for symptomatic pop.Results standardized questionnaires indicated normal genital self-image, sexual satisfaction, and sexual function; however, sexual distress scores exceeded normal cutoffs.Eight themes emerged, including reasons for having sex, willingness to initiate and sexual receptivity, contextual factors, sexual stimuli, sexual arousal, responsive desire, outcomes, and spontaneous sexual desire.In contrast to quantitative findings, themes demonstrated sexual difficulty.Women with pop mainly engage in sex out of obligation and report reduced sexual initiation and receptiveness, as well as a negative impact on genital self-image.Pelvic organ prolapse was perceived to adversely affect subjective arousal, responsive desire, and spontaneous desire, despite intact physiological arousal.Difficulty experiencing subjective arousal was profound and seemed to be limited by preoccupation with pop.Sexual satisfaction and rewards were diminished after pop, including orgasmic capacity.Rewarding motivators to engage in sex were seldom discussed and often overshadowed by experiencing guilt and obligation related to sex.Conclusions the circular sexual response cycle largely fit participants' experiences; however, pop inhibits subjective arousal, which prevents responsive desire for many.Patients may need better support to cope with preoccupation with pop, indicating that addressing the psychosocial symptoms of pop should be prioritized.

Journal: Female pelvic medicine & reconstructive surgery (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Fusion-expressed CtxB-TcpA-C-CPE improves both systemic and mucosal humoral and T-cell responses against cholera in mice.

Development of an effective oral vaccine against cholera, a life-threatening dehydrating diarrheal disease, proved to be a challenging task.To improve oral subunit vaccine immunogenicity and to prevent the state of oral tolerance, application of mucosal adjuvants might be a promising approach.In the present study, the ctx b-tcp a-c-cpe fusion was constructed in which ctx b and c-cpe were used as mucosal adjuvants and vaccine delivery system, respectively, to induce mucosal immune responses, and to improve the anti-toxin and anti-colonizing immunity against v.Cholerae.Materials & methods the fusion construct was synthesized, sub-cloned in p qe30 and expressed in e.Coli.The three antigen, making the fusion protein, were also separately expressed in e.Coli.The recombinant proteins were purified by affinity chromatography using ni-nta agarose.Western blot analysis using anti-his antibody was applied to confirm identity of the purified proteins.Balb/c mice were subcutaneously immunized with ctx b, tcp a, c-cpe and the fusion protein ctx b-tcp a-c-cpe separately.The mice were orally immunized (in 3 boosts) by the same vaccine.Mucosal immune response stimulation was evaluated by measuring the levels of intestinal ig a.Systemic immune response was evaluated by measuring total serum ig g, ig g1, ig g2a, ig g2b subclasses, and also il-4, il-5, il-10 and ifn-γ cytokines in spleen cell culture.Results the recombinant proteins ctx b, tcp a, c-cpe and the fusion protein ctx b-tcp a-c-cpe were expressed in e.Coli and highly purified in a single step of chromatography.Balb/c mice immunized with the fusion protein had highest levels of intestinal ig a, serum ig g and ig g subclasses, compared to each of the three proteins making the fusion.Moreover, stimulated splenocytes of mice immunized with the fusion protein displayed significantly higher amounts of il-5 and ifn-ɣ cytokines.Th2 dominance of the immune response was more evident in mice receiving the fusion protein.Conclusion inclusion of ctx b, as the mucosal adjuvant, and c-cpe, as the vaccine delivery system, in the fusion protein ctx b-tcp a-c-cpe significantly enhanced the elicited mucosal and systemic immune responses, compared to tcp a alone.Of note, significant production of intestinal ig a in mice immunized with the fusion protein is presumably capable of neutralizing tcp a, ctx b and c-cpe antigens, preventing v.Cholera colonization, and toxic function of ctx b and c-cpe.Challenge infection of the immunized mice is required to evaluate protective potential of the fusion protein against v.Cholera.

Journal: Microbial pathogenesis (2021)
Publication types: Journal Article
The Impact of the Degree of Kinesiophobia on Recovery in Patients With Achilles Tendinopathy.

Kinesiophobia has been proposed to influence recovery in individuals with achilles tendinopathy.However, whether there are differences in outcomes in individuals with different levels of kinesiophobia is unknown.The purpose of this study was to compare the characteristics of patients at baseline and recovery over time in individuals with achilles tendinopathy and various levels of kinesiophobia.Methods this study was a secondary analysis of a prospective observational cohort study of 59 individuals with achilles tendinopathy.The participants were divided into 3 groups on the basis of scores on the tampa scale for kinesiophobia (tsk) (those with low tsk scores [≤33; low tsk group], those with medium tsk scores [34-41; medium tsk group], and those with high tsk scores [≥42; high tsk group]).All participants were evaluated with self-reported outcomes, clinical evaluation, tendon morphology, viscoelastic property measurements, and a calf muscle endurance test at baseline, 6 months, and 12 months.No treatment was provided throughout the study period.Results there were 16 participants (8 women) in the low tsk group (age = 51.9 [sd = 15.3] years, body mass index [bmi] = 24.3 [22.3-25.4]), 28 participants (13 women) in the medium tsk group (age = 52.7 [sd = 15.2] years, bmi = 26.4 [22.5-30.8]), and 15 participants (8 women) in the high tsk group (age = 61.1 [sd = 11.1] years, bmi = 28.1 [25.2-33.6]).Among the groups at baseline, the high tsk group had significantly greater bmi and symptom severity and lower quality of life.All groups showed significant improvement over time for all outcomes except tendon viscoelastic properties and tendon thickening when there was an adjustment for baseline bmi.The high and medium tsk groups saw decreases in kinesiophobia at 6 months, but there was no change for the low tsk group.Conclusion despite the high tsk group having the highest bmi and the worse symptom severity and quality of life at baseline, members of this group showed improvements in all of the outcome domains similar to those of the other groups over 12 months.Impact evaluating the degree of kinesiophobia in individuals with achilles tendinopathy might be of benefit for understanding how they are affected by the injury.However, the degree of kinesiophobia at baseline does not seem to affect recovery; this finding could be due to the patients receiving education about the injury and expectations of recovery.

Journal: Physical therapy (2021)
Publication types: Comparative Study;Journal Article;Observational Study;Research Support, N.I.H., Extramural
Coral-ID: A forensically validated genetic test to identify precious coral material and its application to objects seized from illegal traffic.

The production and trade of objects manufactured from the skeletal axis of coralid precious corals is a historically, culturally and economically important global industry.Coralids are members of the diverse coralliidae family, which contains several species complexes and morphospecies.For most precious coral found in the jewelry trade, the color remains the sole clue and link to the taxonomic identity of the individual.Different coralid species have however similar or overlapping colors resulting in difficulty to taxonomically identify jewelry objects, including four species listed by the convention on the international trade of endangered species (cites) whose international transport and trade requires species-specific and country of origin documentation.We aimed at developing a reliable method to taxonomically identify coralid material with the objective of distinguishing cites protected species from their non-protected counterparts.We present coral-id, a genetic assay to taxonomically classify coralid objects using quasi non-destructive sampling.The assay classifies the analyzed sample in one of six taxonomic categories and performs at least presumptive separation of cites-listed and non-listed species in all cases.Developmental validation experiments prove that coral-id is a specific, accurate and very sensitive method.As the first attempt to randomly sample corals in the trade to identify them, we applied coral-id on 20 precious coral objects seized by custom authorities upon import to in switzerland.Thirteen (65%) of these samples could be analyzed; three of these were found to be presumptively cites-listed, and 10 of them have proven to originate from non-cites-listed species.

Journal: Forensic science international. Genetics (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
T cell engineering for adoptive T cell therapy: safety and receptor avidity.

Since the first bone marrow transplantation, adoptive t cell therapy (act) has developed over the last 80 years to a highly efficient and specific therapy for infections and cancer.Genetic engineering of t cells with antigen-specific receptors now provides the possibility of generating highly defined and efficacious t cell products.The high sensitivity of engineered t cells towards their targets, however, also bears the risk of severe off-target toxicities.Therefore, different safety strategies for engineered t cells have been developed that enable removal of the transferred cells in case of adverse events, control of t cell activity or improvement of target selectivity.Receptor avidity is a crucial component in the balance between safety and efficacy of t cell products.In clinical trials, t cells equipped with high avidity t cell receptor (tcr)/chimeric antigen receptor (car) have been mostly used so far because of their faster and better response to antigen recognition.However, over-activation can trigger t cell exhaustion/death as well as side effects due to excessive cytokine production.Low avidity t cells, on the other hand, are less susceptible to over-activation and could possess better selectivity in case of tumor antigens shared with healthy tissues, but complete tumor eradication may not be guaranteed.In this review we describe how 'optimal' tcr/car affinity can increase the safety/efficacy balance of engineered t cells, and discuss simultaneous or sequential infusion of high and low avidity receptors as further options for efficacious but safe t cell therapy.

Journal: Cancer immunology, immunotherapy : CII (2019)
Publication types: Journal Article;Review