syedzayyan/paper_mill_benchmark huggingface dataset:
Structural and dynamic investigation of non-synonymous variations in Renin-AGT complex revealed altered binding via hydrogen-bonding network reprogramming to accelerate the hypertension pathway.

Hypertension is one of the major issues worldwide and one of the main factors involved in heart and kidney failure.Angiotensinogen and renin are key components of the renin-angiotensin-aldosterone system, which plays an indispensable role in hypertension.The aim of this study was to find out the non-synonymous mutations and structure-based mutation-function correlation in the renin-agt complex and reveal the most deleterious mutations to accelerated hypertension.In the current study, we employed computational modeling and molecular simulation approaches to demonstrate the impact of specific mutations in the ren-agt interface in hypertension.Computational algorithms, that is, ph d-snp, poly phen-1, mapp, sorting intolerant from tolerant, screening of non-acceptable polymorphism, predict snp, poly phen-2, and protein analysis through evolutionary relationships predicted 20 mutations as deleterious in agt while only five mutations were confirmed as deleterious in the renin protein.Investigation of the bonding analysis revealed that two mutations s107l and v193f in renin altered the hydrogen-bonding paradigm at the interface site.Furthermore, exploration of structural-dynamic behaviors demonstrated by that these mutations also increases the structural stability to regulate the expression of disease pathway.The flexibility index of each residues and structural compactness analysis further validated the findings by portraying the difference in the dynamic behavior in contrast to the wild type.Binding energy calculations based on molecular mechanics/generalized born surface area methods were used which further established the binding differences between the wild type, s107l, and v193f mutant variants.The total binding energy for wild type, s107l, and v193f was reported to be -27.79, -47.72, and -38.25, respectively.In conclusion, these two mutations increase the binding free energy alongside the docking score to enhance the binding between renin and agt to overexpress this pathway in a hypertension disease condition.Patients with these mutations may be screened for potential therapeutic intervention.

Journal: Chemical biology & drug design (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
End-Of-Life Care in the Time of COVID-19: Communication Matters More Than Ever.

Context the covid-19 pandemic resulted in visitation restrictions across most health care settings, necessitating the use of remote communication to facilitate communication among families, patients and health care teams.Objective to examine the impact of remote communication on families' evaluation of end-of-life care during the covid-19 pandemic.Methods retrospective, cross-sectional, mixed methods study using data from an after-death survey administered from march 17-june 30, 2020.The primary outcome was the next of kin's global assessment of care during the veteran's last month of life.Results data were obtained from the next-of-kin of 328 veterans who died in an inpatient unit (i.e., acute care, intensive care, nursing home, hospice units) in one of 37 va medical centers with the highest numbers of covid-19 cases.The adjusted percentage of bereaved families reporting excellent overall end-of-life care was statistically significantly higher among those reporting very effective remote communication compared to those reporting that remote communication was mostly, somewhat, or not at all effective (69.5% vs.35.7%).Similar differences were observed in evaluations of remote communication effectiveness with the health care team.Overall, 81.3% of family members who offered positive comments about communication with either the veteran or the health care team reported excellent overall end-of-life care vs.28.4% who made negative comments.Conclusions effective remote communication with the patient and the health care team was associated with significantly better ratings of the overall experience of end-of-life care by bereaved family members.Our findings offer timely insights into the importance of remote communication strategies.

Journal: Journal of pain and symptom management (2021)
Publication types: Journal Article
Association of cucurbit aphid-borne yellows virus with cucumber plants in India.

Symptoms like bright yellowing, puckering of the leaf, vein banding, and vein thickening were observed on different cucurbit hosts at the experimental farm of indian agricultural research institute, new delhi during kharif2019.Leaf-dip electron microscopy of the symptomatic leaves revealed the association of isometric virus particles measuring ~ 25 nm with bitter gourd and cucumber samples.The rt-pcr assay using polerovirus generic primers covering the partial rd rp, intergenic region, and partial cp region was resulted the amplicons of ~ 1.1 kb.Subsequent cloning, sequencing, and sequence analysis revealed the association of cucurbit aphid-borne yellows virus (cabyv) with bitter gourd (momordica charantia) and cucumber (cucumis sativus) plants.These results constitute the first report of cabyv infection on cucumber plants from india.Supplementary information the online version of this article () contains supplementary material, which is available to authorized users.

Journal: Virusdisease (2021)
Publication types: Journal Article
Concomitant epicardial left atrial appendage ligation and left atrial ablation of atrial fibrillation: Safety, feasibility and outcome.

Introduction we present initial results of patients undergoing a combined procedure of epicardial laa ligation in addition to left atrial ablation for af.Methods 9 patients were included for additional use of lariat as an individual treatment approach for af.First an epicardial laa ligation was performed, in the same procedure left atrial ablations consisting of pvi and additional substrate based modifying ablations were performed.Follow-up at 3 months and 12 months was performed.Results there was only 1 minor procedural complication (11%) involving epicardial bleeding and 2 late adverse events of pericardial tamponade and stroke.At the final follow-up (median 20 months) 7 patients were in stable sinus rhythm (78%) and 2 pts had reduced af burden.Conclusion concomitant epicardial laa ligation and ablation is feasible in selected patients with a reasonable risk profile.More prospective data are required to validate the safety and efficacy.

Journal: Indian pacing and electrophysiology journal (2021)
Publication types: Journal Article
Relationship of the Pelvic-Trochanteric Index with greater trochanteric pain syndrome.

S this study aimed to correlate a higher pelvic-trochanteric index (pti) with an increased varus of the femoral neck with greater trochanteric pain syndrome (gtps).The secondary objective was to check whether the pelvic width changes with age.Methods a prospective study was conducted to compare female patients diagnosed with gtps (case group) with asymptomatic female participants (control group) from march 2011 to june 2017.On an anteroposterior pelvic radiograph, lines were drawn by two radiologists, and the pti (ratio of the distance between the greater trochanters and distance between the iliac crests) was defined and the femoral neck-shaft angle was measured.Results data collected based on radiographs of 182 female patients (cases) and 150 female participants (controls) showed that the mean pti was 1.09 (sd=0.01) in the case group and 1.07 (sd=0.01) in the control group (p<0.05), regardless of age.The distance between iliac crests increased with age (p<0.05) in symptomatic and asymptomatic individuals.It was also found that the mean femoral neck-shaft angle was 130.6° (sd=0.59) and progression of the varus angulation occurred with age in both groups, with a significance level of 5%.Conclusions the pti was higher in patients with gtps.The femoral neck-shaft angle does not differ between individuals with and without gtps; however, it does decrease with age.The pelvic width tends to increase with aging in symptomatic or asymptomatic individuals; therefore, the increase in the pelvic width and decrease in the femoral neck-shaft angle can be interpreted as normal in aging women, which could alter the biomechanics of the hips and pelvis.

Journal: Clinics (Sao Paulo, Brazil) (2021)
Publication types: Journal Article
Motivators and Barriers to Physical Activity among Youth with Sickle Cell Disease: Brief Review.

Health disparities for minority groups include a low rate of physical activity and underserved urban minority youth with chronic disease are among the least active population segments, as exemplified by sickle cell disease (scd)."exercise prescriptions" for youth with chronic diseases need to be evidence based and align with psychologic motivators and barriers.This scoping review sought evidence for psychosocial motivators or barriers to physical activity (pa) in youth with scd and other chronic disease that could be relevant to scd.Methods five databases were searched for studies on urban minority youth published between 2009 and 2022.Results keyword searching yielded no papers on scd and pa motivation and barriers.Adding health-related quality of life (hrql) in scd found eleven relevant papers.Widening the search to chronic disease in minority youth resulted in a total of 49 papers.Three thematic categories and seven sub-themes emerged.Pa barriers added by chronic disease include fear of triggering disease complications, negative relationships due to disease limitations on performance in sports, and lack of suitable environment for pa that accommodates the chronic disease.Pa motivators are similar for youth without chronic disease: self-efficacy, autonomy, positive relationships with peers and parents and coach/teacher.Conclusion direct descriptions of pa motivations and barriers to pa in scd are limited to fatigue and fear of sickle vaso-occlusive pain.The pa barriers and motivators found for urban youth with chronic disease overlap with themes in healthy adolescents from underserved minorities.Community-based interventions could strengthen pa motivators (self-efficacy, autonomy, positive relationships with peers and parents and coach/teacher) but need disease accommodations to overcome the barriers (fear of triggering disease complications, environmental limitations, and negative relationships).Evidence-based exercise prescriptions might incorporate educational modules to overcome disease stigma and misconceptions.Prospective studies of pa motivators and barriers could improve hrql in scd.

Journal: Children (Basel, Switzerland) (2022)
Publication types: Journal Article;Review
Based on network pharmacology and in vitro experiments to prove the effective inhibition of myocardial fibrosis by Buyang Huanwu decoction.

Among cardiovascular diseases, myocardial fibrosis (mf) is a major pathological change underlying heart failure and is associated with a high mortality rate.However, the molecular mechanism underlying mf has remained elusive.Buyang huanwu decoction (byhwd), a traditional chinese medicine (tcm) formula for cardiovascular diseases, exhibits good anti-inflammatory and blood-activating properties.In the present study, we studied the mf inhibitory effect of byhwd using network pharmacology and experimental validation.We used several databases to collect information on mf and related drugs and finally obtained cross-targets for byhwd and mf.After that we got protein-protein interaction (ppi) network and performed gene ontology (go) and kyoto encyclopedia of genes and genomes (kegg) pathway enrichment analyses to obtain key signaling pathways for further study.After screening, interleukin (il)-6, il-1β, and matrix metallopeptidase 9 (mmp9) were selected forin vitroexperiments, which included cell cycle studies, cell migration rate, quantitative reverse transcription-polymerase chain reaction (q rt-pcr), and western blotting (wb).Finally, molecular docking was performed to validate the results.We found 299 common targets between byhwd and mf.In total, 75 core targets of the ppi core network were selected for enrichment analysis, and the il-17 signaling pathway, which is intricately linked to inflammation, was speculated to be involved.Accordingly,in vitroexperiments were performed.Altogether, our findings indicated that byhwd can affect the function of cardiac fibroblasts and reduce the expression of inflammatory factors in rats.In summary, byhwd can inhibit mf by reducing the expression of inflammatory factors and affecting the il-17 signaling pathway.

Journal: Bioengineered (2022)
Publication types: Journal Article
Interannual fluctuations in connectivity among crab populations (Liocarcinus depurator) along the Atlantic-Mediterranean transition.

An interesting evolutionary question that still remains open is the connectivity between marine populations.Marine currents can favour the dispersal of larvae or adults, but they can also produce eddies and gyres generating oceanographic fronts, thus limiting gene flow.To address this subject, we selected the atlantic-mediterranean transition, where several fronts are located: gibraltar strait (gs), almeria-oran front (aof) and ibiza channel (ic).Seven populations of the marine crab liocarcinus depurator (cadiz, west and east alboran, alacant, valencia, ebro delta and north catalonia) located along this transition were analysed in six consecutive years (2014-2019) using a fragment of the coi (cytochrome oxidase subunit i) gene.All sequences (966) belonged to two well defined haplogroups: atl (most abundant in atlantic waters) and med (predominant in mediterranean waters).Following a geographic variation, the frequency of atl decreased significantly from cadiz to north catalonia.However, this variation presented steps due to the effect of oceanographic restrictions/fronts.Significant effects were recorded for gs (2015, 2017, 2018 and 2019), aof (all years except 2018) and ic (2016).The intensity and precise location of these fronts changed over time.Multivariate analyses distinguished three main population groups: cadiz, alboran sea and the remaining mediterranean populations.These findings could be relevant to properly define marine protected areas and for conservation and fisheries policies.

Journal: Scientific reports (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Mechanisms of Metal-Induced Mitochondrial Dysfunction in Neurological Disorders.

Metals are actively involved in multiple catalytic physiological activities.However, metal overload may result in neurotoxicity as it increases formation of reactive oxygen species (ros) and elevates oxidative stress in the nervous system.Mitochondria are a key target of metal-induced toxicity, given their role in energy production.As the brain consumes a large amount of energy, mitochondrial dysfunction and the subsequent decrease in levels of atp may significantly disrupt brain function, resulting in neuronal cell death and ensuing neurological disorders.Here, we address contemporary studies on metal-induced mitochondrial dysfunction and its impact on the nervous system.

Journal: Toxics (2021)
Publication types: Journal Article;Review
Retro-Tubercle Biplanar Opening Wedge High Tibial Osteotomy Is Favorable for the Patellofemoral Joint But Not for the Osteotomized Tubercle Itself Compared With Supra-Tubercle Osteotomy.

To identify whether retro-tubercle opening-wedge high tibial osteotomy (rt-owhto) produces more favorable radiographic outcomes on patellofemoral joint alignment and clinical outcomes than supra-tubercle opening-wedge high tibial osteotomy (st-owhto).Methods from january 2017 to july 2018, patients who underwent biplanar owhto were allocated to 1 of 2 groups (st-owhto and rt-owhto).Plain radiographs and computed tomography were used to analyze patellofemoral alignment and other radiologic parameters representing osteotomy configurations.Clinical outcomes were assessed using american knee society score and western ontario and mc master universities osteoarthritis index.Results in total, 50 knees that underwent st-owhto and 44 knees that underwent rt-owhto were enrolled.Patellar height was significantly decreased only after st-owhto (caton-deschamps ratio: p = .007; blackburne-peel ratio: p = .012).Patellar tilt angle was decreased in both groups (p = .009 and .004, respectively).Postoperative posterior tibial slope (pts) (p = .013), pts (δ) (p < .001), retro-tuberosity gap distance (p = .001), and retro-tuberosity tip distance (p = .001) were significantly larger in rt-owhto.Retro-tuberosity tip distance was significantly correlated with retro-tuberosity gap distance (p = .002), thickness of second plane osteotomy fragment (p = .027), and anterior osteotomy ratio (p = .031) in st-owhto.In rt-owhto, it was significantly correlated with pts (△) (p < .001), retro-tuberosity gap distance (p < .001), and sagittal angle of bi-planar osteotomy (p = .005).There were 2 cases of tibial tuberosity fracture, 9 cases of delayed union on second plane osteotomy and 5 cases of tuberosity protrusion in rt-owhto.Conclusions although the rt-owhto technique maintains patellofemoral joint alignment, no difference in clinical outcome was detected.The rt-owhto has increased risk of tuberosity fracture, delayed union, and prominent tibial tuberosity.The surgeon should consider these negative aspects of the technique and consider adjusting additional stabilization.Level of evidence level iii, retrospective cohort study.

Journal: Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association (2021)
Publication types: Journal Article
Driving magnetic domains at the nanoscale by interfacial strain-induced proximity.

We investigate the local nanoscale changes of the magnetic anisotropy of a ni film subject to an inverse magnetostrictive effect by proximity to a v2o3 layer.Using temperature-dependent photoemission electron microscopy (peem) combined with x-ray magnetic circular dichroism (xmcd), direct images of the ni spin alignment across the first-order structural phase transition (spt) of v2o3 were obtained.We find an abrupt temperature-driven reorientation of the ni magnetic domains across the spt, which is associated with a large increase of the coercive field.Moreover, angular dependent ferromagnetic resonance (fmr) shows a remarkable change in the magnetic anisotropy of the ni film across the spt of v2o3.Micromagnetic simulations based on these results are in quantitative agreement with the peem data.Direct measurements of the lateral correlation length of the ni domains from xmcd images show an increase of almost one order of magnitude at the spt compared to room temperature, as well as a broad spatial distribution of the local transition temperatures, thus corroborating the phase coexistence of ni anisotropies caused by the v2o3 spt.We show that the rearrangement of the ni domains is due to strain induced by the oxide layers' structural domains across the spt.Our results illustrate the use of alternative hybrid systems to manipulate magnetic domains at the nanoscale, which allows for engineering of coercive fields for novel data storage architectures.

Journal: Nanoscale (2021)
Publication types: Journal Article
The Quality, Intake, and Digestibility of Virginia Fanpetals (SidahermaphroditaL. Rusby) Silage Produced under Different Technologies and Its Effect on the Performance of Young Cattle.

Different harvesting and preservation methods of virginia fanpetals herbage were evaluated, based on the chemical composition and digestible organic matter (om) content (d-value) of silage fed to adult sheep, the intake and digestibility of silage, and the performance of young cattle.The following harvesting methods were compared: direct-cut harvesting with a precision-cut forage harvester (dc), harvesting after field wilting with a precision-cut forage harvester (wc) or a round baler (wrb).The silage was fed for 81 days to 24 polish holstein friesian (hf) bulls, as the sole forage supplemented with 3.0 kg of concentrate/head/day.Harvesting methods affected the density (p< 0.001) and water-soluble carbohydrate (wsc) content (p= 0.047).Differences were found among the groups in the digestibility coefficients of om (dc-73.7, wc-78.9, wrb-79.9%) (p= 0.007), and crude protein (cp) (69.8%, 77.1%, 78.5%, respectively) (p< 0.001).Dry matter intake (dmi) reached 8.38 kg (dc), 8.74 kg (wc) and 7.21 kg (wrb).Live weight gain (lwg) differed (p< 0.001) among groups (0.939, 1.033, 0.813 kg/day, respectively).The feed conversion ratio (fcr) tended to improve in wc (8.66 kg dmi/kg lwg) (p= 0.08).The highest-quality silage was produced in group wc, and it could be successfully fed to growing bulls as the sole forage.

Journal: Animals : an open access journal from MDPI (2021)
Publication types: Journal Article
Frequency of early rapid improvement in stroke severity during interfacility transfer.

As interfacility transfer of patients with stroke becomes increasingly common, understanding fluctuations in deficits during transfer may help predict resource needs.We sought to characterize changes in nih stroke scale (nihss) scores during transfer and identify factors associated with early rapid improvement (eri).Methods we used prospectively collected data from our comprehensive stroke center's (cscs) stroke and telestroke network databases.We calculated changes in nihss scores for all patients transferred to our csc after an initial telestroke evaluation from january 2010 to december 2015.Logistic regression identified factors associated with eri, controlling for patient characteristics available on arrival.Results among the 505 patients included, the median initial nihss score was 11 (interquartile range [iqr] 5-18), and on csc arrival, it was 9 (iqr 3-17), with a median change of 0 (-3 to -0).Of note, 74.5% of scores changed by fewer than 4 points (7% increased ≥4 points, and 19% decreased ≥4).In 85% of cases, the nihss score change did not cross a threshold to alter eligibility for thrombectomy.In multivariable modeling, eri was associated with ability to ambulate before the index stroke (odds ratio [or] 5.79,p= 0.02) and higher initial nihss (or 1.06 per point,p= 0.001).Conclusions these findings may be valuable for resource planning and for inclusion in thrombectomy alert activation processes at the receiving hospital.

Journal: Neurology. Clinical practice (2019)
Publication types: Journal Article
Disclosure of individual research results at federally funded Alzheimer's Disease Research Centers.

Introduction this study describes practices for disclosing individual research results to participants in alzheimer's disease research.Methods an online survey of clinical core leaders at national institutes of health-funded alzheimer's disease research centers in the united states (response rate: 30/31, 97%) examined return of results practices across nine different types of research results.Results most centers had returned consensus research diagnoses (83%) and neuropsychological test results (73%), with fewer having shared amyloid positron emission tomography (43%), tau imaging (10%), or apolipoprotein e (apoe) genotype (7%) results.Centers reported having disclosed a mean of 3.1 types of results (standard deviation = 2.1; range 0-8).The most commonly cited reason for disclosure was to inform participants' medical decision-making (88%).Disclosure involved multiple professionals and modalities, with neurologists (87%) and in-person visits (85%) most commonplace.Discussion centers varied widely as to whether and how they disclosed research results.Diagnostic and cognitive test results were more commonly returned than genetic or biomarker results.

Journal: Alzheimer's & dementia (New York, N. Y.) (2021)
Publication types: Journal Article