syedzayyan/paper_mill_benchmark huggingface dataset:
MicroRNA-346-5p Regulates Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells by Inhibiting Transmembrane Protein 9

The molecular mechanisms how bone marrow‐derived mesenchymal stem cells (bmscs) differentiate into osteoblast need to be investigated.Micro rnas (mi rnas) contribute to the osteogenic differentiation of bmscs.However, the effect of mi r‐346‐5p on osteogenic differentiation of bmscs is not clear.This study is aimed at elucidating the underlying mechanism by which mi r‐346‐5p regulates osteogenic differentiation of human bmscs.Results of alkaline phosphatase (alp) and alizarin red s (ars) staining indicated that upregulation of mi r‐346‐5p suppressed osteogenic differentiation of bmscs, whereas downregulation of mi r‐346‐5p enhanced this process.The protein levels of the osteoblastic markers osterix and runt‐related transcription factor 2 (runx2) were decreased in cells treated with mi r‐346‐5p mimic at day 7 and day 14 after being differentiated.By contrast, downregulation of mi r‐346‐5p elevated the protein levels of osterix and runx2.Moreover, a dual‐luciferase reporter assay revealed that transmembrane protein 9 (tmem9) was a target of mi r‐346‐5p.In addition, the western blot results demonstrated that the tmem9 protein level was significantly reduced by the mi r‐346‐5p mimic whereas downregulation of mi r‐346‐5p improved the protein level of tmem9.These results together demonstrated that mi r‐346‐5p served a key role in bmsc osteogenic differentiation of through targeting tmem9, which may provide a novel target for clinical treatments of bone injury.

Journal: BioMed Research International (2020)
Publication types: Research Article;
Microglia Involves in the Immune Inflammatory Response of Poststroke Depression: A Review of Evidence

Poststroke depression (psd) does not exist before and occurs after the stroke.Psd can appear shortly after the onset of stroke or be observed in the weeks and months after the acute or subacute phase of stroke.The pathogenesis of psd is unclear, resulting in poor treatment effects.With research advancement, immunoactive cells in the central nervous system, particularly microglia, play a role in the occurrence and development of psd.Microglia affects the homeostasis of the central nervous system through various factors, leading to the occurrence of depression.The research progress of microglia in psd has been summarized to review the evidence regarding the pathogenesis and treatment target of psd in the future.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Review Article;
Metformin Ameliorates Senescence of Adipose-Derived Mesenchymal Stem Cells and Attenuates Osteoarthritis Progression via the AMPK-Dependent Autophagy Pathway

Osteoarthritis (oa) is one of the most serious age-related diseases worldwide that drastically affects the quality of life of patients.Despite advancements in the treatment of arthritis, especially with adipose-derived mesenchymal stem cells (adscs), senescence-induced alterations in adscs negatively affect the treatment outcomes.This study was aimed at mechanistically exploring whether metformin could ameliorate the senescence of adscs and at exploring the effect of metformin-preconditioned adscs in an experimental oa mouse model.In this study, an h2o2-induced mouse adsc senescent model was established.Cell proliferation, senescence, and autophagy were investigated in vitro.Moreover, the effects of intra-articular injection of metformin-preconditioned adscs were investigated in vivo.Metformin could promote autophagy and activate the ampk/m tor pathway in adscs.The metformin-enhanced autophagy could improve the survival and reduce the senescence of adscs.The protective effects of metformin against senescence were partially blocked by 3-methyladenine and compound c.Injection of metformin-preconditioned adscs slowed oa progression and reduced oa pain in mice.The results suggest that metformin activates the ampk/m tor-dependent autophagy pathway in adscs against h2o2-induced senescence, while metformin-preconditioned adscs can potentially inhibit oa progression.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Metabonomic Study of the Effect of Dingkundan Intervention Comparing with Oral Contraceptives on Primary Dysmenorrhea Using the UPLC-MS Technique

Primary dysmenorrhea (pd) is a prevalent problem in gynecologic clinics among adolescents and women of reproductive age.Several therapy modalities, including traditional chinese medicine, are deemed adequate (tcm) and have been in practice for a long time.In china, dingkundan (dkd), a multicomponent gynecological treatment, has been used to treat pd for centuries.However, the fundamental process remains poorly understood.Comparing plasma samples acquired from dkd‐treated and oral contraceptive‐ (oc‐) treated subjects, we performed an integrated plasma metabonomic analysis utilizing the uplc‐ms technology to study the therapeutic mechanisms of dkd in pd patients.Thirty possible biomarkers and metabolic pathways were discovered, primarily steroid hormone production, glycerophospholipid metabolism, and bile secretion.The results suggested that dkd may have therapeutic benefits for pd patients via modulation of various metabolic pathways.This study is envisaged to provide detailed metabolite information regarding the etiology of pd, an assessment of the efficacy of dkd, and a comparison of dkd and oc.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Meta-Analysis of the Comprehensive Efficacy of Intraocular Lens Implantation in Glaucoma Patients

This study is aimed at investigating the efficacy of intraocular lens (iol) implantation in patients suffering from glaucoma through meta‐analysis of the previously published research.For this purpose, different literature databases were searched for identification of clinical studies published between january 2000 and january 2022 on evaluating iol’s efficacy in treating glaucoma.Rev man 5.3 was used to conduct a meta‐analysis of the pertinent data.The central anterior chamber depth (acd), corneal endothelial cell counts, best‐corrected visual acuity (bcva), intraocular pressure (iop), anti‐glaucoma medications (agm), and axial length (al) changes were compared, and the incidence of postoperative complications was thoroughly evaluated.The cochran chi‐square test was used to examine the heterogeneity of the evaluation results.According to the inclusion and exclusion criteria, 20 studies included 948 glaucomatous eyes.Iop was significantly lower than before treatment (md = 8.64, 95 ci: 5.75‐11.53; z = 5.86, p &lt; 0.0001), while acd increased significantly (md = −1.38, 95 percent ci: ‐1.74‐1.02; z = 7.49, p &lt; 0.0001).The corneal endothelial cell counts were homogeneous (md = 225.08, 95% ci: ‐64.17 to ‐514.33; z = 1.53, p = 0.20).Agm utilisation decreased (md = 1.43, 95% ci: 0.752.12, z = 4.09, p &lt; 0.0001).Al decreased significantly (md = 0.31; 95% ci: 0.09‐0.54; z = 2.71; p = 0.007).The incidence of complications remained insignificant after iol treatment (or = 1.05, 95% ci: 0.42 to 2.60; z = 0.10, p = 0.92; p = 0.92).These findings indicate that iol treatment can significantly reduce intraocular pressure, glaucoma drug use, and aqueous level (al) in glaucoma patients while increasing the depth of the central anterior chamber.This study offers a theoretical foundation for selecting glaucoma treatment methods.

Journal: BioMed Research International (2022)
Publication types: Meta-Analysis;
Meta-Analysis of Mechanism of Influence of CRY2 on the Differentiation of Mouse Osteoblast through the Regulation of Wnt/Β-Catenin Signaling Pathway

Noncoding rnas were discovered to control a variety of developmental mechanisms, including osteogenesis.According to emerging evidence, cryptochrome circadian‐regulating (cry) proteins have emerged as essential controllers of osteoblast differentiation.The linked processes, on the other hand, are still unknown.The specific process that underpins osteoblast differentiation and proliferation is yet unknown.This research gives a meta‐analysis of cry2’s impact on mouse osteoblast differentiation via the control of the wnt/ β –catenin signaling pathways.Western blot and quantitative real‐time pcr were used to identify cry2 expression levels, components in the osteoblast‐associated signaling pathway, and osteoblast transcription markers.The osteogenic condition was measured utilizing alkaline phosphatase (alp) and alizarin red (ar) staining, whereas cell growth rates were measured using cck8 assays.An ectopic bone formation experiment was used to determine the osteogenic potential of osteoblasts.Cry2 stimulates the osteogenic development of mouse osteoblasts through canonical wnt/ β ‐catenin signaling, according to the findings.

Journal: BioMed Research International (2022)
Publication types: Meta-Analysis;
Meta-Analysis of Matrix Metalloproteinases in the Risk of Cardiovascular and Neurodegenerative Diseases

To investigate the risk of cardiovascular and neurodegenerative diseases induced by matrix metalloproteinases (mmps) by meta‐analysis.Methods .Relevant literature was searched from wanfang medical center, cnqi, vip, pub med, and other domestic and foreign literature databases for the research direction, and the risk of cardiovascular and neurodegenerative diseases induced by mmps was meta‐analyzed using the fixed‐effect model and random‐effect model.Results .Mmp‐1 and mmp‐9 were risk factors for cardiovascular diseases by fixed and random‐effect model analysis, respectively, while mmp‐2 and mmp‐9 were risk factors for increased neurodegenerative diseases by random‐effect model analysis.Conclusion .Mmp‐1 and mmp‐9 are risk factors for cardiovascular diseases, and mmp‐2 and mmp‐9 are major factors for increased risk of neurodegenerative diseases.Mmp‐1, mmp‐2, and mmp‐9 can be used as new targets for clinical diagnosis, treatment, and research of subsequent cardiovascular and neurodegenerative diseases.

Journal: BioMed Research International (2022)
Publication types: Meta-Analysis;
Mesenchymal Stem Cell-Derived Exosomes and Their Potential Agents in Hematological Diseases

Mesenchymal stem cells (mscs) are the most exploited stem cells with multilineage differentiation potential and immunomodulatory properties.Numerous lines of findings have reported their successful applications in a multitude of inflammatory conditions and immune disorders.However, it is currently discovered that these effects are mainly mediated in a paracrine manner by msc‐exosomes.Moreover, msc‐exosomes have been implicated in a wide variety of biological responses including immunomodulation, oxidative stress, tumor progression, and tissue regeneration.Meanwhile, they are reported to actively participate in various hematological diseases by the means of transferring different types of exosomal components to the target cells.Therefore, in this review, we briefly discuss the sources and biological features of mscs and then illustrate the biogenesis and biological processes of msc‐exosomes.Of note, this paper especially highlights the latest research progress of msc‐exosomes in hematological diseases.

Journal: Oxidative Medicine and Cellular Longevity (2021)
Publication types: Research Article;
MCU That Is Transcriptionally Regulated by Nrf2 Augments Malignant Biological Behaviors in Oral Squamous Cell Carcinoma Cells

To clarify the role and molecular mechanism of mitochondrial calcium uniporter (mcu) in the malignant biological behaviors of oral squamous cell carcinoma (oscc) cells through clinical and cellular experiments.Methods .Immunohistochemistry and q rt‐pcr techniques were used to observe the expression of mcu, nuclear factor erythroid 2‐related factor 2 (nrf2), mitochondrial calcium uptake 1 (micu1), and micu2 in oscc and normal tissues.After treatment with si‐mcu, spermine, and/or sh‐nrf2, malignant biological behaviors of oscc cells including proliferation, migration, and apoptosis were detected by clone formation, migration, and mitochondrial membrane potential (mmp) assays.Furthermore, mcu, micu1, micu2, nrf2, and other proteins related to malignant biological behaviors were examined using western blot, immunohistochemistry, and immunofluorescence assays.Results .Mcu, nrf2, and micu1 were strongly expressed in oscc as compared to normal tissues, while micu2 was relatively weakly expressed in oscc tissues.Knockdown of mcu distinctly weakened proliferation and migration and lowered mmp level in cal 27 cells.Conversely, its activation reinforced migrated capacity and increased mmp level in cal 27 cells, which was reversed after cotransfection with sh‐nrf2.After treatment with si‐mcu or spermine, nrf2 expression was not affected in cal 27 cells.However, mcu expression was distinctly suppressed in cal 27 cells transfected with sh‐nrf2.Furthermore, knockdown of nrf2 significantly reversed the increase in expression of micu1 and micu2 induced by mcu activation in cal 27 cells.Conclusion .Mcu, as a novel oncogene of oscc, augments malignant biological behaviors of oscc cells, which could be transcriptionally regulated by nrf2.

Journal: BioMed Research International (2021)
Publication types: Research Article;
Marine-Derived Piericidin Diglycoside S18 Alleviates Inflammatory Responses in the Aortic Valve via Interaction with Interleukin 37

Calcific aortic valve disease (cavd) is a valvular disease frequently in the elderly individuals that can lead to the valve dysfunction.Osteoblastic differentiation of human aortic valve interstitial cells (havics) induced by inflammation play a crucial role in cavd pathophysiological processes.To date, no effective drugs for cavd have been established, and new agents are urgently needed.Piericidin glycosides, obtained from a marine-derived streptomyces strain, were revealed to have regulatory effects on mitochondria in previous studies.Here, we discovered that 13-hydroxypiericidin a 10-o-α-d-glucose (1→6)-β-d-glucoside (s18), a specific piericidin diglycoside, suppresses lipopolysaccharide- (lps) induced inflammatory responses of havics by alleviating mitochondrial stress in an interleukin (il)-37-dependent manner.Knockdown of il-37 by si rna not only exaggerated lps-induced havic inflammation and mitochondrial stress but also abrogated the anti-inflammatory effect of s18 on havics.Moreover, s18 alleviated aortic valve lesions in il-37 transgenic mice of cavd model.Microscale thermophoresis (mst) and docking analysis of five piericidin analogues suggested that diglycosides, but not monoglycosides, exert obvious il-37-binding activity.These results indicate that s18 directly binds to il-37 to alleviate inflammatory responses in havics and aortic valve lesions in mice.Piericidin diglycoside s18 is a potential therapeutic agent to prevent the development of cavd.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Magnetic Resonance Imaging Features in Diagnosis of Breast Cancer and Evaluation of Effect of Epidermal Growth Factor Receptor-Targeted Therapy

Breast cancer diagnosis and treatment are important healthcare issues in the industrialized world.In this study, magnetic resonance imaging (mri) images are used to diagnose breast cancer (bc) and analyze the application effect of epidermal factor receptor‐targeted therapy in the treatment of bc.A total of 858 patients diagnosed with bc in jincheng people’s hospital from 2019 to 2021 are included and randomly divided into an experimental group and a control group.The experimental group is treated with epidermal growth factor receptor‐ (egfr‐) targeted therapy, and the control group is treated with conventional chemotherapy according to subsequent treatment modalities.Experimental results show that compared with manual segmentation, machine automatic segmentation includes the local and edge information of the image with higher accuracy, compared with benign and malignant tumors.There are significant differences in the changes of tpeak, ssmax, el, e 2, and e 5, p &lt; 0.05, with ssmax and el having the greatest changes.After chemotherapy, the recorded maximum diameter of cancer foci in the control and the experimental groups are 26.4 ± 11.6 mm and 20.3 ± 13.5 mm, respectively, and the difference is statistically meaningful ( p &lt; 0.05).The adc value (12.74 ± 2.08) in the experimental group is higher than that (9.7 ± 1.88) in the control group ( p &lt; 0.05).There is a significant difference in ssmax between the control group and experimental group ( p &lt; 0.05), the simax, p h values of the control group are significantly higher than those of the experimental group ( p &lt; 0.05), and the ssmax r value (7.82 ± 6.24) in the experimental group is lower than that in the control group (10.08 ± 6.25), but the difference is not significant, p &gt; 0.05.The proposed mri method has high sensitivity and accuracy in the diagnosis of bc, improving the detection rate of lesions, compared with conventional chemotherapy.In addition, epidermal factor receptor‐targeted therapy has a better therapeutic effect, with significant changes in cancer foci, which has the value of clinical promotion.

Journal: BioMed Research International (2022)
Publication types: Clinical Study;
Macrophage Rmp Ameliorates Myocardial Infarction by Modulating Macrophage Polarization in Mice

Inflammation plays important roles during myocardial infarction (mi).Macrophage polarization is a major factor that drives the inflammatory process.Our previous study found that rna polymerase ii subunit 5-mediating protein (rmp) knockout in cardiomyocytes caused heart failure by impairing mitochondrial structure and function.However, whether macrophage rmp plays a role in mi has not been investigated.Methods.Macrophage rmp-knockout in combination with a mouse model of mi was used to study the function of macrophage rmp in mi.Next, we modified bone marrow-derived macrophages (bmdms) by plasmid transfection, and the bmdms were administered to lys m-cre/dtr mice by tail vein injection.Immunoblotting and immunofluorescence were used to detect macrophage polarization, fibrosis, angiogenesis, and the p38 signaling pathway in each group.Results.Macrophage rmp deficiency aggravates cardiac dysfunction, promotes m1 polarization, and inhibits angiogenesis after mi.However, rmp overexpression in macrophages promotes m2 polarization and angiogenesis after mi.Mechanistically, we found that rmp regulates macrophage polarization through the heat shock protein 90– (hsp90–) p38 signaling pathway.Conclusions.Macrophage rmp plays a significant role in mi, likely by regulating macrophage polarization via the hsp90–p38 signaling pathway.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Long Noncoding RNAs: New Regulators of Resistance to Systemic Therapies for Gastric Cancer

Gastric cancer (gc) is the second leading cause of cancer mortality and the fourth most commonly diagnosed malignant disease, with approximately 951,000 new cases diagnosed and approximately 723,000 cases of mortality each year.The highest mortality rate of gc is in east asia, and the lowest is in north america.A large number of studies have demonstrated that gc patients are characterized by higher morbidity, metastasis rates, and mortality and lower early diagnosis rates, radical resection rates, and 5‐year survival rates.All cases of gc can be divided into two important stages, namely, early‐ and advanced‐stage gc, and the stage mainly determines the treatment strategy for and the therapeutic effect in gc patients.Patients with early‐stage gc undergo radical surgery followed by chemotherapy, and the 5‐year survival rate can be as high as 90%.However, patients with advanced‐stage gc cannot undergo radical surgery because they are at risk for metastasis; therefore, they can choose only radiotherapy or chemotherapy and have a poor prognosis.Based on the lack of specific clinical manifestations and detection methods, most gc patients (&gt;70%) are diagnosed in the advanced stage; therefore, continued efforts toward developing treatments have been focused on advanced‐stage gc patients and include molecular targeted therapy, immunotherapy, and small molecular therapy.Nevertheless, in recent years, accumulating evidence has indicated that small molecules, especially long noncoding rnas (lnc rnas), are involved in the occurrence, development, and progression of gc, and their abundantly dysregulated expression has been identified in gc tissues and cell lines.Therefore, lnc rnas are considered easily detectable molecules and ideal biomarkers or target‐specific agents for the future diagnosis or treatment of gc.In this review, we primarily discuss the status of gc, the role of lnc rnas in gc, and the emerging systemic treatments for gc.

Journal: BioMed Research International (2021)
Publication types: Review Article;
Long Noncoding RNA TUG1 Aggravates Cerebral Ischemia/Reperfusion Injury by Acting as a ceRNA for miR-3072-3p to Target St8sia2

Long noncoding rna taurine-upregulated gene 1 (tug1) is considered to be involved in postischemic cerebral inflammation, whereas polysialic acid (poly sia, psa), the product of st8sia2, constitutes polysialylated neural adhesion cell molecule (psa-ncam) in both mice and humans and that cerebral psa-ncam level is elevated in neuronal progenitor cells in response to transient focal ischemia.Herein, we aim to identify novel mi rnas that bridge the functions of st8sia2 and tug1 in ischemia-associated injuries.In both in vivo (c57bl/6j mouse ischemia/reperfusion, i/r model) and in vitro (mouse neuroblastoma n2a cell oxygen glucose deprivation/reoxygenation, ogd model) settings, we observed upregulated tug1 and st8sia2 after the induction of ischemic injury, accompanied by reduced mi r-3072-3p expression.We performed si rna-induced tug1 knockdown combined with the induction of ischemic injury; the results showed that inhibiting tug1 expression led to the reduced infarct area and improved neurological deficit.Through bioinformatics analysis, mi r-3072-3p was found to target both st8sia2 and tug1, which was subsequently verified by the luciferase reporter system and rna binding protein immunoprecipitation assay.Also, the addition of mi r-3072-3p mimic/inhibitor resulted in reduced/elevated st8sia2 expression at the protein level.Further studies revealed that in both in vivo and in vitro settings, tug1 bound competitively to mi r-3072-3p to regulate st8sia2 expression and promote apoptosis.In summary, targeting the tug1/mi r-3072-3p/st8sia2 regulatory cascade, a novel cascade we identified in cerebral ischemia injury, may render feasible therapeutic possibilities for overcoming cerebral ischemic insults.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Long Noncoding RNA SNHG12 Promotes Prostate Tumor Occurrence and Progression via AKT Regulation

The small nucleolar rna host gene 12 (snhg12) has been reported to play an important role in the tumorigenesis and progression of pca, but the functional underlying mechanism has not been studied clearly.We detected the expression level of snhg12 in pca tissues and matched adjacent normal tissues that were collected from 85 patients.Then, colony formation assays, mtt experiments, and flow cytometry were used to examine the effect of snhg12 on proliferation, cell cycle distribution, and apoptosis of du145 cells.Further, transwell invasion assay was utilized to assess whether snhg12 participates in pca cell invasion and affects the secretion of vegf secretion in du145 cells.Finally, we investigated the effect of snhg12 on tumor growth in vivo .We found that snhg12 promoted cell proliferation and suppressed apoptosis in pca cells, which suggests that snhg12 is probably a novel pca biomarker and therapy target of pca.

Journal: BioMed Research International (2020)
Publication types: Research Article;