syedzayyan/paper_mill_benchmark huggingface dataset:
Effects of Pulsed Radiofrequency on Nerve Repair and Expressions of GFAP and GDNF in Rats with Neuropathic Pain

To study the effect of pulsed radio frequency (prf) on nerve repair and the expression of gfap and gdnf in rats with neuropathic pain.Methods .Thirty spf healthy sd rats were randomly divided into control group (group c), psnl group (partial ligation of sciatic nerve) + sham group (group ps), and psnl group (partial ligation of sciatic nerve) + prf group (group pr), with 10 rats in each group.In group c, the right sciatic nerve was exposed without ligation.In the ps group, the model of neuropathic pain was established by partial ligation of sciatic nerve.The mice in the pr group were treated with prf after establishing the neuropathic pain model.The general behavior of rats during the treatment was observed.The mechanical and thermal hyperalgesia were measured before operation and 1, 3, 7, and 14 days after operation.The content of inflammatory factors in nerve tissue was detected by elisa.The pathological condition of nerve tissue was observed by he.The gene and protein changes of gfap and gdnf in nerve tissue were determined by qrt pcr and western blot.Results .Rats in the control group were free to move and in good condition.In the ps group, there were different degrees of claudication, weakness of the lower limbs, lateral toe valgus, nerve injury, and other behavioral changes.After the pulsed radiofrequency in the pr group, the above symptoms decreased gradually with the prolongation of the treatment time.The mechanical pain sensitivity and thermal allodynia of the ps group were reduced after the operation.The mechanical pain sensitivity and thermal pain sensitivity of the pr group gradually increased with the prolongation of the treatment time, and the 14 days were basically close to the control group.The levels of tnf‐ α and il‐6 in elisa were significantly higher in the ps group than in the control group, and the content in the pr group was gradually reduced, which was close to the control group.He staining showed that the sciatic nerve fibers disappeared, and the formation of nerve cavities was obvious in the 14‐day ps group.The nerve fibers were found in the sciatic tissue of the pr group, and there was no obvious hemorrhagic edema and cell deformation.The expression of gfap m rna in the ps group was significantly higher than that in the control group and the pr group ( p &lt; 0.05), and the expression of gdnf was opposite ( p &lt; 0.05).The results of western blot showed that the expression of gfap protein in the 14‐day ps group was significantly higher than that in the control group.The expression of the pr group decreased compared with the control group, and the expression of gdnf was opposite ( p &lt; 0.05).Conclusion .Pulsed radiofrequency ablation can promote neurological repair, promote gdnf, and reduce the expression of gfap in rats with neuropathic pain.

Journal: BioMed Research International (2021)
Publication types: Research Article;
Image Super-Resolution Reconstruction Method for Lung Cancer CT-Scanned Images Based on Neural Network

The super‐resolution (sr) reconstruction of a single image is an important image synthesis task especially for medical applications.This paper is studying the application of image segmentation for lung cancer images.This research work is utilizing the power of deep learning for resolution reconstruction for lung cancer‐based images.At present, the neural networks utilized for image segmentation and classification are suffering from the loss of information where information passes through one layer to another deep layer.The commonly used loss functions include content‐based reconstruction loss and generative confrontation network.The sparse coding single‐image super‐resolution reconstruction algorithm can easily lead to the phenomenon of incorrect geometric structure in the reconstructed image.In order to solve the problem of excessive smoothness and blurring of the reconstructed image edges caused by the introduction of this self‐similarity constraint, a two‐layer reconstruction framework based on a smooth layer and a texture layer is proposed for a medical application of lung cancer.This method uses a global nonzero gradient number constrained reconstruction model to reconstruct the smooth layer.The proposed sparse coding method is used to reconstruct high‐resolution texture images.Finally, a global and local optimization models are used to further improve the quality of the reconstructed image.An adaptive multiscale remote sensing image super‐division reconstruction network is designed.The selective core network and adaptive gating unit are integrated to extract and fuse features to obtain a preliminary reconstruction.Through the proposed dual‐drive module, the feature prior drive loss and task drive loss are transmitted to the super‐resolution network.The proposed work not only improves the subjective visual effect but the robustness has also been enhanced with more accurate construction of edges.The statistical evaluators are used to test the viability of the proposed scheme.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Identification and Validation of Key Genes in Hepatocellular Carcinoma by Bioinformatics Analysis

Hepatocellular carcinoma (hcc) is the most frequent primary liver cancer and has poor outcomes.However, the potential molecular biological process underpinning the occurrence and development of hcc is still largely unknown.The purpose of this study was to identify the core genes related to hcc and explore their potential molecular events using bioinformatics methods.Hcc‐related expression profiles gse25097 and gse84005 were selected from the gene expression omnibus (geo) database, and the differentially expressed genes (degs) between 306 hcc tissues and 281 corresponding noncancerous tissues were identified using geo2r online tools.The protein‐protein interaction network (ppin) was constructed and visualized using the string database.Gene ontology (go) and kegg pathway enrichment analyses of the degs were carried out using david 6.8 and kobas 3.0.Additionally, module analysis and centrality parameter analysis were performed by cytoscape.The expression differences of key genes in normal hepatocyte cells and hcc cells were verified by quantitative real‐time fluorescence polymerase chain reaction (q rt‐pcr).Additionally, survival analysis of key genes was performed by gepia.Our results showed that a total of 291 degs were identified including 99 upregulated genes and 192 downregulated genes.Our results showed that the ppin of hcc was made up of 287 nodes and 2527 edges.Go analysis showed that these genes were mainly enriched in the molecular function of protein binding.Additionally, kegg pathway analysis also revealed that degs were mainly involved in the metabolic, cell cycle, and chemical carcinogenesis pathways.Interestingly, a significant module with high centrality features including 10 key genes was found.Among these, cdk1, ndc80, hmmr, cdkn3, and pttg1, which were only upregulated in hcc patients, have attracted much attention.Furthermore, q rt‐pcr also confirmed the upregulation of these five key genes in the normal human hepatocyte cell line (hl‐7702) and hcc cell lines (smmc‐7721, mhcc‐97l, and mhcc‐97h); patients with upregulated expression of these five key genes had significantly poorer survival and prognosis.Cdk1, ndc80, hmmr, cdkn3, and pttg1 can be used as molecular markers for hcc.This finding provides potential strategies for clinical diagnosis, accurate treatment, and prognosis analysis of liver cancer.

Journal: BioMed Research International (2021)
Publication types: Research Article;
Effects of Torreya grandis Kernel Oil on Lipid Metabolism and Intestinal Flora in C57BL/6J Mice

Recent experimental studies have shown that vegetable oil supplementation ameliorates high-fat diet- (hfd-) induced hyperlipidemia and oxidative stress in mice via modulating hepatic lipid metabolism and the composition of the gut microbiota.The aim of this study was to investigate the efficacy of the torreya grandis kernel oil (tko) rich in unpolysaturated fatty acid against hyperlipidemia and gain a deep insight into its potential mechanisms.Methods.Normal mice were randomly divided into three groups: nd (normal diet), lo (normal diet supplement with 4% tko), and ho (normal diet supplement with 8% tko).Hyperlipidemia mice were randomly divided into two groups: hfn (normal diet) and hfo (normal diet supplement with 8% tko).Blood biochemistry and histomorphology were observed; liver rna-seq, metabolomics, and gut 16s r rna were analyzed.Results.Continuous supplementation of tko in normal mice significantly ameliorated serum total cholesterol (tc), triglyceride (tg), high-density lipoprotein cholesterol (hdl-c), low-density lipoprotein cholesterol (ldl-c), and free fatty acid (ffa) accumulation, decreased blood glucose and malondialdehyde (mda), and enhanced superoxide dismutase (sod) and glutathione peroxidase (gsh-px) levels.According to go and kyoto encyclopedia of genes and genomes (kegg) analysis, most differentially expressed genes (degs) were significantly enriched in the biosynthesis of unsaturated fatty acid pathways, and significantly changed metabolites (scms) might be involved in the metabolism of lipids.High-dose tko improved gut alpha diversity and beta diversity showing that the microbial community compositions of the five groups were different.Conclusion.Supplementation of tko functions in the prevention of hyperlipidemia via regulating hepatic lipid metabolism and enhancing microbiota richness in normal mice.Our study is the first to reveal the mechanism of tko regulating blood lipid levels by using multiomics and promote further studies on tko for their biological activity.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Effects of Ginsenoside Rg1 on the Biological Activity of Human Periodontal Ligament Cells

objective .To investigate the effect of ginsenoside rg1 on the biological activity of primary cultured human periodontal ligament cells (pdlc).Methods .The effects of ginsenoside rg1 on the proliferation activity, protein synthesis, and alkaline phosphatase (alp) activity of primary cultured human periodontal ligament cells were investigated by thiazole blue (mtt) colorimetric method, coomassie brilliant blue method, and enzyme kinetics method.The effect of ginsenoside rg1 on cell cycle was detected by flow cytometry, and the cells were labeled with calcium ion‐sensitive fluorescent probe fluo3/am, and the effect of ginsenoside rg1 on intracellular free calcium concentration was detected by laser scanning confocal microscope.Results .Compared with the control group, the experimental groups of ginsenoside rg1 at various concentrations could significantly promote cell proliferation, and the effect time was the longest in the concentration range of 0.01‐0.05 μ mol/l;, rg1 0.01umol/l and 0.05umol/l.The protein content in the 72‐hour cell culture medium of the μ mol/l group was significantly higher than that of the control group; the alp activity in the 72‐hour cell culture medium of the rg1 0.01 μ mol/l, 0.05 μ mol/l, and 0.1 μ mol/l groups was significantly higher than that of the control group; fcm assay showed that after 0.1 μ mol/l rg1 for 48 hours, compared with the control group, the proportion of cells in the early stage of dna synthesis (g1%) of pdlc was significantly reduced, while the proportion of cells in the dna synthesis stage (s%) and the value of cell proliferation index pr i (s + g2m)% were significantly increased; rg1 increased intracellular calcium in pdlc cells at first and then decreased and finally maintained at a slightly higher resting calcium level than before drug addition.Conclusion .Ginsenoside rg1 can increase the proliferation activity, protein synthesis, and alkaline phosphatase activity of periodontal ligament cells within a certain concentration range; rg1 reduces the cells in g1 phase and increases cells in s phase of periodontal ligament fibroblasts.Change the concentration of free calcium ions in cells and promote more cells to enter a proliferative state.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Effects of Aspirin on Myocardial Ischemia-Reperfusion Injury in Rats through STAT3 Signaling Pathway

To investigate the role and mechanism of aspirin in myocardial injury induced by myocardial ischemia‐reperfusion in rats through stat3 signaling pathway.Methods .Sixty rats were randomly divided into three groups: the sham operation group, mi/r group, and mi/r+aspirin group (aspirin group).The rats in the sham operation group did not ligate the lad coronary artery, while the aspirin group ligated the lad coronary artery, which caused the suture to be loosened after 30 minutes ischemia, and 60 mg/kg aspirin was injected into the tail vein 10 minutes before reperfusion.After three hours of reperfusion, the ultrasound system was used to collect hemodynamic parameters, including ejection fraction (ef%), shortening fraction (fs%), and left ventricular end‐systolic pressure (lvesp%) and left ventricular end‐diastolic pressure (lvedp%).Finally, the rats were euthanized; then, blood samples were taken for biochemical examination, myocardial tissue was collected, and the left ventricle was used for subsequent experiments.The gene expression levels of bax and bcl‐2 were detected by pcr.The protein expression levels of bcl‐2, bax, p‐jak2, total jak2, p‐stat3, and total stat3 were detected by western blot.Results .Compared with the sham operation group and the aspirin group, the area of myocardial infarction in the mi/r was significantly increased ( p &lt; 0.05).In terms of hemodynamic parameters, lvedp was significantly elevated in the mi/r group.The results of pcr showed that compared with the mi/r group, the m rna expression of bax in the aspirin group was significantly decreased, while that of bcl‐2 was significantly increased ( p &lt; 0.05).Western blot analysis showed that compared with the mi/r group, aspirin pretreatment significantly increased the expression levels of p‐stat3 and p‐jak2 ( p &lt; 0.05).Conclusion .The mechanism of aspirin preconditioning to protect the heart from mi/r injury appears to be related to jak2/stat3 and related to the activation of the signaling pathway.

Journal: BioMed Research International (2021)
Publication types: Research Article;
Effect of Vegetable Waste on Growth Performance and Hematology of Broiler Chicks

Vegetable waste (spinach, potato, and cauliflower) is a rich and natural source of nutrients, potentially good for supplying minerals, essential amino acids, and antioxidants to the birds.Relatively, its cost very low, easily to accessible, easily process &amp; pose little risk of illness.The aim of present study was to evaluate the effect of vegetable waste (vw) as feed supplement on growth performance and hematology of broiler chicks.For this purpose, a total of 200 (4 days old) vaccinated chicks were acquired from a commercial hatchery multan which was acclimated for three weeks (21 days) on basal starter feed after that 25‐day‐old chicks with uniform body weight were allocated according to a crd (completely randomized design) into four dietary treatments with three replicates of each contained 15 chicks in 12 pens.In dietary treatments, chicks were feed with basal feed (bf) and supplemented feed with vegetable waste (vw) of spinach, potato, and cauliflower.For this purpose, the dietary treatments included control treatment ( t 1 ) (100% bf+0% vw) and other dietary treatments ( t 2 ) (75% bf+25% vw), ( t 3 ) (50% bf+50% vw), and ( t 4 ) (25% bf+75% vw).The body weight, feed intake, food conversion ratio (fcr), and mortality were checked on weekly and daily basis.For hematology analysis, after the 1st experimental week (25‐day‐old chicks) and the last 5th experimental week (56‐day‐old chicks), the samples of blood were gathered from the wing’s veins of two birds from each treatment in random way.At the end of five weeks (35 days), birds with uniform average body weight were selected per treatment with three replicates (2 bird/replicate) and then were manually slaughtered according to the halal method to analyze the weight of internal body organs of broilers by physical and statistical analysis (anova).There was no significant effect ( p &gt; 0.05) on feed intake and fcr among all the dietary treatments.But in average, body weight and bwg were higher in treatment ( t 2 ) ( p &lt; 0.01) than all other dietary treatments ( t 3 ) and ( t 4 ) and control treatment ( t 1 ).The blood constituents in this study showed that broilers in control treatment ( t 1 ) and other dietary treatments ( t 2 ), ( t 3 ), and ( t 4 ) fed on different doses were significantly ( p &lt; 0.01) different from each other.The week 5 ( w 5 ) shows higher values of blood constituents ( p &lt; 0.01) than week 1 ( w 1 ).The carcass yield of the chicks fed on different doses showed that they were significantly different ( p &lt; 0.01) among the dietary treatments.The vw inclusion 0%, 25%, 50%, and 75% had positive effect on blood constituents and carcass yield of the broiler chicks; they were significantly ( p &lt; 0.01) different among the treatments.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Effect of Transdermal Fentanyl Patch Combined with Enhanced Recovery after Surgery on the Curative Effect and Analgesic Effect of Liver Cancer

Its goal was to see how a transdermal fentanyl patch combined with accelerated recovery after surgery (eras) affected the treatment efficacy and analgesic effect of liver cancer, as well as to help patients with liver cancer choose the right analgesic treatment and nursing mode.150 patients with liver cancer were divided into group a (transdermal fentanyl patch), group b (eras), and group c (transdermal fentanyl patch combined with eras).Patients in the three groups were compared in terms of pain, survival, psychological status, adverse responses, postoperative recovery, and patient satisfaction.The results showed that under different treatment and nursing methods, the number of patients with mild cancer pain in the three groups was increased, especially the number of patients with mild cancer pain in group c ( p &lt; 0.05).Besides, the quality of life score of patients in each group was decreased.Patients who received the combination analgesia had a significantly higher quality of life than those who received simply a transdermal fentanyl patch or eras ( p &lt; 0.05).The scores of both the hamilton anxiety scale (hama) and hamilton depression rating scale (hamd) of patients with the combined analgesia were decreased signally ( p &lt; 0.05).There were few patients with combined analgesia who had adverse reactions ( p &lt; 0.05).After surgery, the time of the first anal exhaust, first defecation, and first ambulation in group c were shorter than those in the other two groups ( p &lt; 0.05).To summarize, combining the two techniques aided in the recovery of gastrointestinal function as well as the physical recovery of patients following surgery.Furthermore, combining the two approaches produced a clear analgesic impact, which could improve patients’ quality of life while also having a favorable clinical adoption effect.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Effect of lncRNA PVT1/miR186/KLF5 Axis on the Occurrence and Progression of Cholangiocarcinoma

This study primarily focused on the effect of the long noncoding rna (lnc rna) pvt1/mi r186/klf5 axis on the occurrence and progression of cholangiocarcinoma (cca).Mi r186 was found both in the lnc rna pvt1 targeting mi rnas and klf5 targeting mi rnas using bioinformatic analysis.The expression of lnc rna pvt1 and klf5 in the tfk‐1, qbc939, and hu cct1 cell lines and normal biliary epithelial hibepi c cells was detected by rt‐q pcr.The significance of lnc rna pvt1 and klf5 on cell proliferation was analyzed using the mtt assay and clone formation assay in lnc rna pvt1 and klf5 silencing hu cct1 cell lines and lnc rna pvt1and klf5 overexpressing tfk‐1 and qbc939 cell lines, respectively.The potential role of lnc rna pvt1 and klf5 in cell migration was detected using the transwell invasion assay in cca cell lines and tumor formation assay.Additionally, lnc rna pvt1 and klf5 were proved to be highly expressed in cca tissues and cell lines.Silencing and overexpressing of lnc rna pvt1 or klf5 markedly inhibited or increased the cell proliferation and cell invasion in cca cell lines, respectively.Silencing and overexpressing of lnc rna pvt1 significantly inhibited and increased the expression of klf5 in cca cell lines, respectively.Silencing of lnc rna pvt1 increased the expression of mi r186, and silencing of mi r186 increased the expression of klf5 in cca cell lines.Cotransfection of lnc rna pvt1 and mi r186 increased the expression of klf5 compared with controls.Overall, these results demonstrated that the lnc rna pvt1/mi r186/klf5 axis might exert a key role in the occurrence and progression of cca, and this axis might provide a new target for treating cca.

Journal: BioMed Research International (2021)
Publication types: Research Article;