syedzayyan/paper_mill_benchmark huggingface dataset:
Prediction of autistic tendencies at 18 months of age via markerless video analysis of spontaneous body movements in 4-month-old infants.

Early intervention is now considered the core treatment strategy for autism spectrum disorders (asd).Thus, it is of significant clinical importance to establish a screening tool for the early detection of asd in infants.To achieve this goal, in a longitudinal design, we analyzed spontaneous bodily movements of 4-month-old infants from general population and assessed their asd-like behaviors at 18 months of age.A total of 26 movement features were calculated from video-recorded bodily movements of infants at 4 months of age.Their risk of asd was assessed at 18 months of age with the modified checklist for autism in toddlerhood, a widely used screening questionnaire.Infants at high risk for asd at 18 months of age exhibited less rhythmic and weaker bodily movement patterns at 4 months of age than low-risk infants.When the observed bodily movement patterns were submitted to a machine learning-based analysis, linear and non-linear classifiers successfully predicted asd-like behavior at 18 months of age based on the bodily movement patterns at 4 months of age, at the level acceptable for practical use.This study analyzed the relationship between spontaneous bodily movements at 4 months of age and the asd risk at 18 months of age.Experimental results suggested the utility of the proposed method for the early screening of infants at risk for asd.We revealed that the signs of asd risk could be detected as early as 4 months after birth, by focusing on the infant's spontaneous bodily movements.

Journal: Scientific reports (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Prophylactic ligation of the opacified thoracic duct in minimally invasive esophagectomy - feasibility and safety.

Chyle leak is a rare but morbid complication of esophagectomy.We assessed the feasibility of visualization and prophylactic ligation of the opacified thoracic duct (td) after administration of 50 ml of olive oil.Methods this prospective single center study considered all patients with carcinoma of the middle and lower thirds of the thoracic esophagus including the gastroesophageal junction (gej), managed from january 2018 to december 2019, for inclusion.All patients underwent mc keown minimally invasive esophagectomy.After anesthesia and endotracheal intubation, 50 ml of olive oil was administered through a nasogastric (ng) tube.During thoracoscopic esophageal mobilization, the opacified thoracic duct was identified and ligated using weck hem-o-lok clips immediately above the diaphragmatic hiatus.Postoperatively, the nature, volume, and triglyceride levels of the fluid from the chest drain were recorded.Results forty-three patients with carcinoma of the esophagus were assessed for inclusion and eventually, 33 were enrolled.The median age of the study population was 55 years, and there were 20 males.The tumor site was the lower esophagus in 24 (72.7%) patients.The most common histolopathological finding was squamous cell carcinoma (97%).The opacified thoracic duct could be identified and ligated in 31 (93.9%) patients.The median duration from the administration of olive oil to the ligation of the thoracic duct was 100 min.The median chest drain output and triglyceride levels on postoperative day (pod) one were 250 ml and 48 mg% respectively.No patient developed postoperative chylothorax.Conclusion opacification and visualization of the thoracic duct during thoracoscopy can be aided by administering olive oil.Ligation of this opacified duct is feasible and safe.

Journal: Langenbeck's archives of surgery (2021)
Publication types: Journal Article
Improving the system power of complex kinship analysis by combining multiple systems.

Complex kinship analysis is a critical issue in forensic genetics.To address this issue, 55 strs and 94 snps collected from four commercial forensic typing kits [three kits were based on a capillary electrophoresis (ce) platform and one was based on a next-generation sequencing (ngs) platform] were employed to test the system power for 2nd-degree and 3rd-degree kinship analysis.To measure the kinship index in related individuals, likelihood ratios (lrs) were calculated based on length and sequence polymorphism information (lrlengthand lrsequence, respectively) from simulation as well as true pedigree samples.Lrs calculated based on sequence information are generally higher than those based on length information.The sensitivity, specificity, and effectiveness to distinguish the 2nd- and 3rd-degree kinship were estimated from four marker sets with different numbers of markers.As expected, system power for kinship analysis improved by increasing the number of markers and using lrsequence, instead of lrlength.Furthermore, the system power based on 55 strs from the ce platform is equal to the 40 strs and 94 snps from one ce kit and the kit based on ngs platform for both 2nd-degree and 3rd-degree kinship analysis.For discrimination of 2nd-degree kinship, the system effectiveness is 86.63% with an error ratio < 0.01 using the 55 strs from the ce platform.Using sequence information from the 55 strs and 94 snps, the system effectiveness is 94.43%, with an error ratio < 0.001 for 2nd-degree kinship analysis and 64.34% with an error ratio < 0.05 for 3rd-degree kinship analysis, indicating that these markers are powerful for 2nd-degree kinship analysis and can be used for 3rd-degree kinship analysis.

Journal: Forensic science international. Genetics (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Complete Genome Sequence from an Uncultivated FreshwaterElusimicrobiotaLineage.

Here, we report the first complete genome of an uncultivated freshwater elusimicrobiotaorganism recovered from a nonaxenic amoebozoasp.Culture.The chromosome was obtained from a metagenomic long-read sequencing run and was assembled as a circular element at a 47× coverage, a length of 3.8 mbp, and a g+c content of 68.6%.

Journal: Microbiology resource announcements (2022)
Publication types: Journal Article
Coffee intake may promote sudomotor function activationviathe contribution of caffeine.

S to determine whether drinking coffee with caffeine accelerates the sympathetic response to acetylcholine (ach).Methods tests were performed twice at 1-week intervals following the intake of coffee.Subjects were randomly divided into two groups: group a was administered 16 fluid oz of water (con), while group b was given 16 fluid oz of coffee (coffee).After 1 week, group a was administered 16 fluid oz of coffee (coffee), while group b was given 16 fluid oz of water (con).The quantitative sudomotor axon reflex test (qsart) was performed after intake of coffee and water and a 40 min break.Qsart with iontophoresis and 10% ach was performed to determine axon reflex (axr) mediated with and without iontophoresis [axr (1) and axr (2), respectively], and directly activated sweating (dir).Results the sweat onset time of the axr was shorter in the coffee compared with the con (p< 0.05).The sweat rates in axr (1) axr (2) and dir were significantly higher in the coffee than in the con (p< 0.05,p< 0.05,p< 0.01, respectively).In addition, the coffee showed significantly higher density of activated sweat glands and activated sweat gland output than the con (p< 0.05,p< 0.01, respectively).The overall results of this study showed that coffee intake could stimulate higher activation in both axr and dir sweat responses.Conclusion coffee intake can improve sweating sensitivity in both the axr and dir by the contribution of caffeine contained in coffee.This suggests that other compounds in coffee may not inhibit the sympathetic response to ach.Therefore, coffee may be clinically worth considering as a supplement for the activation of the cholinergic and sudomotor function.

Journal: Frontiers in nutrition (2022)
Publication types: Journal Article
High acuity sleeve gastrectomy patients in a free-standing ambulatory surgical center.

Procedures performed in ambulatory surgical centers (asc) can provide several advantages over hospital-based surgery.Understandably, concerns have been raised regarding "high acuity" cases in the asc setting.Recently the metabolic and bariatric surgery accreditation and quality improvement program (mbsaqip) presented protocols for ascs to follow, requiring them to perform only "low acuity" cases to be compliant with accreditation.Objective assess the safety and efficacy of outpatient sleeve gastrectomy (sg) on the "high acuity patient" in a free-standing asc.Setting free-standing asc, eviva bariatrics, seattle, washington.Methods data were collected retrospectively for all patients who underwent sleeve gastrectomy from january 1, 2013 to december 31, 2015, n = 1112.Of those patients, 120 were classified as "high acuity." results mean age was 51.7 years (24-73), mean body mass index was 42.4 (26.2-65.9).Mean operative time was 91 minutes.Five patients (4.2%) were readmitted within 30 days.Causes of re-admission were portal vein thrombosis (n = 2), intra-abdominal abscess (n = 1), infected hematoma (n = 1), and postoperative bleeding (n = 1).One patient (0.83%) was transferred from the asc to a nearby hospital due to a postoperative bleed.One patient (0.83%) had a re-operation to evacuate a hematoma.One patient had a re-operation to wash out an infected hematoma.There were 0 confirmed staple line leaks.There were no open conversions and no deaths within 30 days or at 1 year.Follow-up was 83% (n = 100) at 6 months, and 65.0% at 1 year (n = 78).Conclusion criteria such as age, body mass index, or prior bariatric surgery did not reflect worse outcomes in a specialized asc.With experienced surgeons, appropriate protocols, and a consistent operative team, sg can be performed safely in a free-standing asc on select "high acuity" patients.

Journal: Surgery for obesity and related diseases : official journal of the American Society for Bariatric Surgery (2017)
Publication types: Journal Article
Exploration of psychological distress experienced by survivors of adolescent cancer reporting a need for psychological support.

In this qualitative study, we aimed to provide an in-depth exploration of cancer-related psychological distress experienced by young survivors of cancer during adolescence reporting a need for psychological support.Methods two individual interviews were held with ten young survivors of cancer diagnosed in adolescence.The interviews were audio-recorded and transcribed verbatim.Analysis followed the guidelines for inductive qualitative manifest content analysis.Results the survivors described distress experienced during and after the end of treatment.Five categories comprising 14 subcategories were generated.The categories included: a tough treatment, marked and hindered, not feeling good enough, struggling with the fragility of life, and finally, an ongoing battle with emotions.Conclusion young survivors of adolescent cancer reporting a need for psychological support described feeling physically, socially, and mentally marked by the cancer experience.They struggled with powerlessness, insecurity, social disconnection, loneliness, and feelings of being unimportant and a failure, and had difficulties understanding and managing their experiences.These concerns should be addressed in psychological treatments for the population irrespective of which approach or model is used to understand survivors' difficulties.A trans-diagnostic approach targeting processes that underpin different manifestations of distress may be effective.

Journal: PloS one (2018)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Phylogeny and functional diversity of halophilic microbial communities from a thalasso environment.

The el-rawda solar saltern, located in north sinai, egypt, is formed through the process of water evaporation from the bradawil lagoon.This evaporation leads to the precipitation of gypsum, halite minerals, and salt flats, which subsequently cover the southern and eastern areas of the lagoon.This study employed the shotgun metagenomic approach, the illumine platform, and bioinformatic tools to investigate the taxonomic composition and functional diversity of halophilic microbial communities in solar saltern.The metagenomic reads obtained from the brine sample exhibited a greater count compared to those from the sediment sample.Notably, the brine sample was primarily characterized by an abundance of archaea, while the sediment sample displayed a dominant abundance of bacteria.Both samples exhibited a relatively low abundance of eukaryotes, while viruses were only found in the brine sample.Furthermore, the comparative analysis of functional pathways showed many important processes related to central metabolism and protein processing in brine and sediment samples.In brief, this research makes a valuable contribution to the understanding of very halophilic ecosystems in egypt, providing insights into their microbial biodiversity and functional processes.

Journal: Saudi journal of biological sciences (2023)
Publication types: Journal Article
Risk factors of postoperative delirium after cardiac surgery: a meta-analysis.

Postoperative delirium is a frequent event after cardiac surgery.This meta-analysis aimed to identify relevant risk factors.Method in this meta-analysis, all original researches regarding patients undergoing mixed types of cardiac surgery (excluding transcatheter procedures) and postoperative delirium were evaluated for inclusion.On july 28th 2020, we searched pub med, embase, web of science and scopus.Data about name of first author, year of publication, inclusion and exclusion criteria, research design, setting, method of delirium assessment, incidence of delirium, odds ratio (or) and corresponding 95% confidence interval (ci) of risk factors, and other information relevant was collected.Or and 95% ci were used as metrics for summarized results.Random effects model was applied.Results fourteen reports were included with a total sample size of 13,286.The incidence of delirium ranged from 4.1 to 54.9%.Eight risk factors were identified including aging, diabetes, preoperative depression, mild cognitive impairment, carotid artery stenosis, nyha functional class iii or iv, time of mechanical ventilation and length of intensive care unit stay.Conclusion in this study several risk factors associated with postoperative delirium after cardiac surgery were identified.Utilizing the information may allow us to identifying patients at high risk of developing postoperative delirium prior to delirium onset.

Journal: Journal of cardiothoracic surgery (2021)
Publication types: Journal Article;Meta-Analysis;Review
NEUROPATHOLOGIC FINDINGS IN CETACEANS STRANDED IN ITALY (2002-14).

We summarized the neuropathologic findings in 60 cetaceans stranded along the italian coastline from 2002 to 2014.The following neuropathologic changes were detected in 45% (27/60) of animals: nonsuppurative meningo-encephalitides (30%, 18/60), nonspecific lesions (12%, 7/60), suppurative encephalitis (2%, 1/60), and neoplasm (2%, 1/60).No histologic lesions were found in 47% (28/60) of the specimens.Five (8%, 5/60) samples were unsuitable for analysis.Analysis with pcr detected brucella spp., morbillivirus, and toxoplasma gondii infection in one, six, and seven individuals, respectively.Immunohistochemical analysis confirmed positivity for morbillivirus and for t.Gondii infection in three cases each.No evidence of the scrapie-associated prion protein pr psc was detected.Our findings underscore the importance of an adequate surveillance system for monitoring aquatic mammal pathologies and for protecting both animal and human health.

Journal: Journal of wildlife diseases (2018)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Do Invasive and Naturalized Aphid Pest Populations Respond Differently to Climatic and Landscape Factors?

Ongoing environmental change affects pest populations, migration, and propensity to damage crops, but the responses to climatic drivers could vary among newly invasive and already naturalized closely related species.To compare these responses of a newly invasive aphid, metopolophium festucae cerealium (stroyan), with its naturalized congeneric [m.Dirhodum (walker)] and confamilial [sitobian avenae (fab.)], we conducted annual surveys over four years across a total of 141 winter wheat fields in the inland pacific northwest, usa.Key climatic factors (cumulative precipitation for each calendar year to sampling date, cumulative degree days), landscape factors (proportion of wheat and landscape diversity within the sample year), and julian day were calculated for each sampling event, and aphid abundance by species, total aphid abundance, overall species richness, diversity, and aphid community composition were assessed.Metopolophium f.Cerealium, the second most abundant species, was positively associated with precipitation, suggesting a projected increase in precipitation in winter and spring in the region could favor its establishment and expansion.Although m.Dirhodum and s.Avenae linearly (positively) associated with temperature, m.F.Cerealium did not, indicating that continued warming may be detrimental to the species.Despite the weak impacts of landscape factors, our study indicated that more wheat generally facilitates cereal aphid abundance.Metopolophium f.Cerealium abundance tended to be higher in earlier (may/early june vs.Late june/july) samples when wheat crop could be vulnerable to aphid feeding.This study suggests that the new presence of m.F.Cerealium has important pest management implications in the region.

Journal: Journal of economic entomology (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't;Research Support, U.S. Gov't, Non-P.H.S.
Isolation of Hepatocytes from Liver Tissue by a Novel, Semi-Automated Perfusion Technology.

Primary hepatocytes are a major tool in biomedical research.However, obtaining high yields of variable hepatocytes is technically challenging.Most protocols rely on the two-step collagenase perfusion protocol introduced by per ottar seglen in 1976.In this procedure, the liver is perfused in situ with a recirculating, constant volume of calcium-free buffer, which is maintained at 37 °c and continuously oxygenated.In a second step, the liver is removed from the carcass and perfused with a collagenase solution in order to dissociate the extracellular matrix of the liver and liberate individual cells.Finally, the dissected hepatocytes are further purified and concentrated by density-based centrifugation.However, failure in proper cannulation, incomplete enzymatic digestion or over-digestion can result in low cell yield and viability.Here we present a novel semi-automated perfusion device, which allows gentle, rapid and efficient generation of a single-cell suspension from rodent livers.In combination with prefabricated buffers, the system allows reliable and highly reproducible isolation of primary hepatocytes.

Journal: Biomedicines (2022)
Publication types: Journal Article
Microwave-Assisted Extraction of Bioactive Compounds from Lentil Wastes: Antioxidant Activity Evaluation and Metabolomic Characterization.

The recovery of industrial by-products is part of the zero-waste circular economy.Lentil seed coats are generally considered to be a waste by-product.However, this low-value by-product is rich in bioactive compounds and may be considered an eco-friendly source of health-promoting phytochemicals.For the first time, a sustainable microwave-assisted extraction technique was applied, and a solvent screening was carried out to enhance the bioactive compound content and the antioxidant activity of green and red lentil hull extracts.With respect to green lentil hull extracts that were obtained with different solvents, the aqueous extract of the red lentil seed coats showed the highest total phenolic and total flavonoid content (tpc = 28.3 ± 0.1 mg gae/g dry weight, tfc = 1.89 ± 0.01 mg ce/100 mg dry weight, respectively), as well as the highest antioxidant activity, both in terms of the free radical scavenging activity (abts, 39.06 ± 0.73 mg te/g dry weight; dpph, ic50= 0.39 μg/m l) and the protection of the neuroblastoma cell line (sh-sy5y, ic50= 10.1 ± 0.6 μg/m l), the latter of which has never been investigated so far.Furthermore, a metabolite discovery analysis was for the first time performed on the aqueous extracts of both cultivars using an hplc separation which was coupled with an orbitrap-based high-resolution mass spectrometry technique.

Journal: Molecules (Basel, Switzerland) (2022)
Publication types: Journal Article
Assessing New Technologies in Surgery: Case Example of Acute Primary Repair of the Thumb Ulnar Collateral Ligament.

Health technology assessment provides a means to assess the technical properties, safety, efficacy, cost-effectiveness, and ethical/legal/social impact of a novel technology.An important component of health technology assessment is the cost-effectiveness analysis (cea), which can be performed using model-based cea.This study used the cea model to compare the cost-effectiveness of a novel ligament augmentation device with the standard technique for primary repair of complete ulnar collateral ligament (ucl) tears.Methods a model was developed for complete ucl tear requiring acute surgical repair, comparing the cost-effectiveness of standard technique primary repair and repair using a ligament augmentation device from a societal perspective.Primary outcomes included quality-adjusted life years (qalys), cost, net monetary benefit (nmb) and incremental nmb.A cost-effectiveness threshold of cad $50,000/qaly was used to compare the 2 techniques.Sensitivity analyses were conducted to assess the parameter uncertainty, specifically the impact of device cost, time off work, probability of complication, and postoperative outcome.Results the nmb for the standard technique was cad $42,598, and the nmb for repair using the ligament augmentation device was cad $41,818.The standard technique was the preferred strategy for primary repair of complete ucl tears.One-way sensitivity analyses demonstrated that the ligament augmentation device became cost-effective if individuals return to work in <18 days (base case 23 days).The device was also favored when the cost was less than cad $50 and the difference in time to return to work was at least 1 day.Conclusions our model demonstrates that there may be significant costs associated with the introduction of novel health technologies, and certain conditions, such as an earlier return to work, must be met for some devices to be a cost-effective option.This study provides an example of how model-based cea is a useful tool to assess the cost-effectiveness of a novel device.Type of study/level of evidence economic/decision analysis ii.

Journal: The Journal of hand surgery (2021)
Publication types: Journal Article
Synthesis of Trisubstituted Furans via Copper(I)-Catalyzed Strain-Driving Cycloisomerization/Annulative Fragmentation.

Thein situformed furan-fused cyclobutenes via cu(i)-catalyzed cycloisomerization of readily available allenyl ketones bearing a cyclopropyl moiety are a highly reactive and powerful species, which undergo annulative fragmentation with terminal ynones to afford a wide variety of functional furans in moderate to high yields.This ring-distortion protocol features an unprecedented strain-controlled cycloisomerization/diels-alder/retro-diels-alder (cdrd) sequence under mild conditions.

Journal: Organic letters (2022)
Publication types: Journal Article