syedzayyan/paper_mill_benchmark huggingface dataset:
IL-33 induces thymic involution-associated naive T cell aging and impairs host control of severe infection.

Severe infection commonly results in immunosuppression, which leads to impaired pathogen clearance or increased secondary infection in both humans and animals.However, the exact mechanisms remain poorly understood.Here, we demonstrate that il-33 results in immunosuppression by inducing thymic involution-associated naive t cell dysfunction with aberrant expression of aging-associated genes and impairs host control of infection in mouse disease models of schistosomiasis or sepsis.Furthermore, we illustrate that il-33 triggers the excessive generation of medullary thymic epithelial cell (m tec) iv (thymic tuft cells) in a pou2f3-dependent manner, as a consequence, disturbs m tec/cortical tec (c tec) compartment and causes thymic involution during severe infection.More importantly, il-33 deficiency, the anti-il-33 neutralizing antibody treatment, or il-33 receptor st2 deficient thymus transplantation rescues t cell immunity to better control infection in mice.Our findings not only uncover a link between severe infection-induced il-33 and thymic involution-mediated naive t cell aging, but also suggest that targeting il-33 or st2 is a promising strategy to rejuvenate t cell immunity to better control severe infection.

Journal: Nature communications (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Scrotal subcutaneous temperature is increased by scrotal insulation or whole-body heating, but not by scrotal neck insulation; however, all three heat-stress models decrease sperm quality in bulls and rams.

Ruminant testes are ~2-6 °c below body temperature; increased testicular temperature reduces sperm motility and morphology.Our objective was to serially monitor scrotal subcutaneous temperatures during testicular heat stress and relate those to sperm quality.Two experiments were conducted, with temperature sensors surgically implanted in scrotal subcutaneous tissues recording temperatures every 15 min and semen collected and evaluated weekly.After an initial control interval, testicular temperature was increased.In experiment 1, in two angus bulls, whole-scrotum insulation for 96 h increased scrotal subcutaneous temperatures by ~2.0-2.5 °c (p < 0.05).Total and progressive motility decreased (p < 0.05) and reached a nadir at week 3 (~20 and 10%, respectively).Furthermore, morphologically normal sperm and acrosome integrity also decreased significantly, reaching nadirs at weeks 3 (15%) and 4 (34%).In experiment 2, 10 dorset rams were allocated randomly into two equal groups and either: 1) exposed to 28 °c ambient temperature and 30-34% rh for 8 h/d for 4 d; or 2) subjected to scrotal neck insulation that was applied and removed at the same time as the start and completion, respectively, of heat exposures in the other rams.Scrotal subcutaneous temperatures (monitored in three rams per group) were increased in response to whole-body heating (~0.8 °c, p < 0.05), but not significantly changed by scrotal neck insulation.Decreases in sperm quality were generally similar between treatments, with the most profound changes evident 4 wk after heat stress, with ~10 percentage point reductions in both total and progressive motility and ~10 and 20 percentage point decreases in morphologically normal sperm in rams with whole-body heating versus scrotal neck insulation, respectively.In conclusion, scrotal subcutaneous temperature was significantly increased by scrotal insulation or whole-body heating, but not by scrotal neck insulation; however, all three heat-stress models decreased sperm motility and morphology in bulls and rams.

Journal: Journal of thermal biology (2021)
Publication types: Journal Article
Low-energy Pulsed Electromagnetic Field Therapy Reduces Pain in Fibromyalgia: A Randomized Single-blind Controlled Pilot Study.

S fibromyalgia symptoms have a significant impact on the quality of life and respond poorly to medications.It has been hypothesized that the use of low-energy pulsed electromagnetic field (pemf) induces neuroprotective effects that may interfere with pain perception.We explored the efficacy of pemf in patients affected by fibromyalgia.Methods twenty-one females (median age 59 years, interquartile range [iqr] 16.5) affected by fibromyalgia were randomized to receive pulsed electromagnetic field-triple energy pain treatment (pemf-tept) or placebo at t0 and at 4 weeks and 8 weeks.Fibromyalgia impact questionnaire (fiq), widespread pain index (wpi), visual analog score (vas) pain, symptom severity (ss) scale, and short form 36 (sf-36) health survey questionnaire have been evaluated.Results patients in the pemf-tept group had a significantly higher reduction of wpi compared to placebo (mean difference -12.90 ± standard deviation [sd] 5.32 vs.-1.91 ± 4.55, difference in difference [dd] of -10.99;p< 0.001), of ss score (-4.10 ± 4.85 vs.-2.00 ± 2.32; dd = -2.1;p< 0.05), of vas pain (-48 ± 30.75 vs.-16.82 ± 23.69; dd = -31.18;p< 0.01).They also reported a higher improvement of fiq and sf-36, albeit not reaching statistical significance.Conclusion in our pilot controlled study, pemf-tept appeared to be safe and improved fibromyalgia symptoms.

Journal: Rheumatology and immunology research (2022)
Publication types: Journal Article
Optimal Anticoagulant Strategy for Periprocedural Management of Atrial Fibrillation Ablation: A Systematic Review and Network Meta-Analysis.

This network meta-analysis was performed to rank the safety and efficacy of periprocedural anticoagulant strategies in patients undergoing atrial fibrillation ablation.Medline, embase, central, and web of science were searched to identify randomized controlled trials comparing anticoagulant regimens in patients undergoing atrial fibrillation ablation up to july 1, 2021.The primary efficacy and safety outcomes were thromboembolic and major bleeding events, respectively, and the net clinical benefit was investigated as the primary-outcome composite.Seventeen studies were included (n= 6950).The mean age ranged from 59 to 70 years; 74% of patients were men and 55% had paroxysmal atrial fibrillation.Compared with the uninterrupted vitamin-k antagonist strategy, the odds ratios for the composite of primary safety and efficacy outcomes were 0.61 (95%ci: 0.31-1.17) with uninterrupted direct oral anticoagulants, 0.63 (95%ci: 0.26-1.54) with interrupted direct oral anticoagulants, and 8.02 (95%ci: 2.35-27.45) with interrupted vitamin-k antagonists.Uninterrupted dabigatran significantly reduced the risk of the composite of primary safety and efficacy outcomes (odds ratio, 0.21; 95%ci, 0.08-0.55).Uninterrupted direct oral anticoagulants are preferred alternatives to uninterrupted vitamin-k antagonists.Interrupted direct oral anticoagulants may be feasible as alternatives.Our results support the use of uninterrupted direct oral anticoagulants as the optimal periprocedural anticoagulant strategy for patients undergoing atrial fibrillation ablation.

Journal: Journal of clinical medicine (2022)
Publication types: Journal Article;Review
Carbon-Coated ZnS-FeS2Heterostructure as an Anode Material for Lithium-Ion Battery Applications.

The construction of carbon-coated heterostructures of bimetallic sulfide is an effective technique to improve the electrochemical activity of anode materials in lithium-ion batteries.In this work, the carbon-coated heterostructured zn s-fe s2is prepared by a two-step hydrothermal method.The crystallinity and nature of carbon-coating are confirmed by the investigation of xrd and raman spectroscopy techniques.The nanoparticle morphology of zn s and plate-like morphology of fe s2is established by tem images.The chemical composition of heterostructure zn s-fe s2@c is discovered by an xps study.The cv results have disclosed the charge storage mechanism, which depends on the capacitive and diffusion process.The bet surface area (37.95 m2g-1) and lower rctvalue (137 ω) of zn s-fe s2@c are beneficial to attain higher lithium-ion storage performance.It delivered a discharge capacity of 821 m ah g-1in the 500th continuous cycle @ a g-1, with a coulombic efficiency of around 100%, which is higher than the zn s-fe s2heterostructure (512 m ah g-1).The proposed strategy can improve the electrochemical performance and stability of lithium-ion batteries, and can be helpful in finding highly effective anode materials for energy storage devices.

Journal: International journal of molecular sciences (2022)
Publication types: Journal Article
Involvement of small-conductance Ca2+-activated K+(SKCa2) channels in spontaneous Ca2+oscillations in rat pinealocytes.

Melatonin secretion from the pineal glands regulates circadian rhythms in mammals.Melatonin production is decreased by an increase in cytosolic ca2+concentration following the activation of nicotinic acetylcholine receptors in parasympathetic systems.We previously reported that pineal ca2+oscillations were regulated by voltage-dependent ca2+channels and large-conductance ca2+-activated k+(bkca) channels, which inhibited melatonin production.In the present study, the contribution of small- and intermediate-conductance ca2+-activated k+(skcaand ikca) channels to the regulation of spontaneous ca2+oscillations was examined in rat pinealocytes.The amplitude and frequency of spontaneous ca2+oscillations were increased by a skcachannel blocker (100 n m apamin), but not by an ikcachannel blocker (1 μm tram-34).On the other hand, they were decreased by a skcachannel opener (100 μm dcebio), but not by an ikcachannel opener (1 μm dcebio).Expression analyses using quantitative real-time pcr, immunocytochemical staining, and western blotting revealed that the skca2 channel subtype was abundantly expressed in rat pinealocytes.Moreover, the enhanced amplitude of ca2+oscillations in the presence of apamin was further increased by a bkcachannel blocker (1 μm paxilline).These results suggest that the activity of skca2 channels regulates cytosolic ca2+signaling and melatonin production during parasympathetic activation in pineal glands.

Journal: Biochemical and biophysical research communications (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Immunomodulatory roles of selenium nanoparticles: Novel arts for potential immunotherapy strategy development.

Current chemotherapy strategies used in clinic appear with lots of disadvantages due to the low targeting effects of drugs and strong side effects, which significantly restricts the drug potency, causes multiple dysfunctions in the body, and even drives the emergence of diseases.Immunotherapy has been proved to boost the body's innate and adaptive defenses for more effective disease control and treatment.As a trace element, selenium plays vital roles in human health by regulating the antioxidant defense, enzyme activity, and immune response through various specific pathways.Profiting from novel nanotechnology, selenium nanoparticles have been widely developed to reveal great potential in anticancer, antibacterial, and anti-inflammation treatments.More interestingly, increasing evidence has also shown that functional selenium nanoparticles can be applied for potential immunotherapy, which would achieve more effective treatment efficiency as adjunctive therapy strategies for the current chemotherapy.By directly interacting with innate immune cells, such as macrophages, dendritic cells, and natural killer cells, selenium nanoparticles can regulate innate immunity to intervene disease developments, which were reported to boost the anticancer, anti-infection, and anti-inflammation treatments.Moreover, selenium nanoparticles can also activate and recover different t cells for adaptive immunity regulations to enhance their cytotoxic to combat cancer cells, indicating the potential of selenium nanoparticles for potential immunotherapy strategy development.Here, aiming to enhance our understanding of the potential immunotherapy strategy development based on se nps, this review will summarize the immunological regulation effects of selenium nanoparticles and the application of selenium nanoparticle-based immunotherapy strategies.Furthermore, we will discuss the advancing perspective of selenium nanoparticle-based potential immunotherapy as a kind of novel adjunctive therapy to enhance the efficiency of current chemotherapies and also introduce the current obstacles for the development of selenium nanoparticles for potential immunotherapy strategy development.This work is expected to promote the future research on selenium nanoparticle-assisted immunotherapy and finally benefit the more effective disease treatments against the threatening cancer and infectious and chronic diseases.

Journal: Frontiers in immunology (2022)
Publication types: Journal Article;Review;Research Support, Non-U.S. Gov't
The FBN rat model of aging: investigation of ABR waveforms and ribbon synapse changes.

Age-related hearing loss is experienced by one-third of individuals aged 65 years and older and can be socially debilitating.Historically, there has been poor correlation between age-related threshold changes, loss of speech understanding, and loss of cochlear hair cells.We examined changes in ribbon synapse number at four different ages in fisher brown norway rats, an extensively studied rat model of aging.In contrast to previous work in mice/wistar rats, we found minimal ribbon synapse loss before 20 months, with significant differences in 24- and 28-month-old rats at 4 k hz.Significant outer hc loss was observed at 24 and 28 months in low- to mid-frequency regions.Age-related reductions in auditory brainstem response wave i amplitude and increases in threshold were strongly correlated with ribbon synapse loss.Wave v/i ratios increased across age for click, 2, 4, and 24 k hz.Together, we find that ribbon synapses in the fisher brown norway rat cochlea show resistance to aging until ∼60% of their life span, suggesting species/strain differences may underpin decreased peripheral input into the aging central processor.

Journal: Neurobiology of aging (2018)
Publication types: Journal Article;Research Support, N.I.H., Extramural;Research Support, U.S. Gov't, Non-P.H.S.
Suppression of reproductive behaviour and gonadal function in female horses-An update.

The behaviour of mares is often detrimental to their performance resulting in frequent demand for methods to suppress gonadal function.In addition, prevention of unintended reproduction especially in feral horse populations may require methods for suppression of gonadal function.Surgical ovariectomy is a safe method but not an acceptable approach in feral mares and undesired in mares where future breeding is considered.There are different approaches for artificial prolongation of the luteal phase resulting in transient inhibition of oestrus and ovulation.Among those, treatment with natural or synthetic progestogens is considered the most common and successful method.Whereas application of intrauterine devices may result in prolongation of luteal function in non-pregnant mares, intrauterine insertion of glass balls is no longer recommended because of complications in individual mares.There are several safer alternatives that may be of interest, especially for population control in free-roaming horses.Treatment with long-acting deslorelin implants inhibited ovulation and oestrus behaviour in mares for limited and variable time intervals in a dose-dependent manner.The effect of gn rh vaccines varies considerably among individual mares, is age dependent, and oestrus-like behaviour may still occur.Contraception via immunization against native porcine or recombinant zona pellucida antigen is successful, but immunocontraception is as much a result of ovarian inactivity as an antibody-based block to sperm-oocyte binding.In conclusion, several treatments for suppression of gonadal function in mares are available, but there are advantages and disadvantages associated that have to be considered.The treatment of choice will thus differ with regard to the demands.

Journal: Reproduction in domestic animals = Zuchthygiene (2022)
Publication types: Journal Article;Review
Which GOLD B patients progress to GOLD D with the new classification?

The 2017 gold guidelines revised assessment of copd by eliminating the fev1criterion.Aim first, we explored the redistribution of 2011 gold groups by reference to the 2017 gold criteria.Second, we investigated the characteristics of gold b patients and the natural course of gold b patients according to the 2017 gold guidelines.Methods in total, 2,010 copd patients in the korean copd subgroup study cohort were analyzed at baseline and 1 year after enrollment.Results the 2011 gold c patients were redistributed to the 2017 a (64.5%) and c (35.4%) groups.The 2011 gold d patients were redistributed to the 2017 b (61.6%) and d (38.6%) groups.The gold b patients constituted 62.7% of all patients according to the 2017 classification.Such patients exhibited higher % predicted fev1values, longer six-minute walk distances, fewer symptoms, and lower inflammatory marker levels than gold d patients.Most gold b patients remained in that group (69.1%), but 13.8% progressed to group d at 1-year follow-up.The factors associated with progression from gold b to gold d were older age, higher modified medical research council (m mrc) and st george's respiratory questionnaire (sgrq) symptom scores, and a lower % predicted fev1value.Conclusion severe symptoms, poorer health status, and greater airflow limitation increased patients' risk of exacerbation and progression from group b to group d when the 2017 gold criteria were applied.

Journal: International journal of chronic obstructive pulmonary disease (2018)
Publication types: Journal Article
Cost-effective imprinting to minimize consumption of template in room-temperature ionic liquid for fast purification of chlorogenic acid from the extract of E. ulmoides leaves.

One of the main challenges in large-scale applications of molecularly imprinted polymers (mips) is the significant amount of template needed in polymer preparation.A new strategy based on room-temperature ionic liquids (rtils) was suggested to solve this problem by reducing the amount of template in the polymerization recipe.The mip was synthesized with a mixture of dimethyl sulfoxide and rtil (1-butyl-3-methylimidazolium tetrafluoroborate) as porogen, in which chlorogenic acid (cga) was used as template, 4-vinylpyridine (4-vp) as functional monomer, and ethylene glycol dimethacrylate (edma) as cross-linker.The influence of polymerization variables, including cga concentrations, and the ratio of 4-vp to edma on imprinting effect were investigated comprehensively.Moreover, the properties involving the column permeability, the number of binding sites, and the polymer morphology of the cga-mip monoliths were studied thoroughly.The mip monolith had an excellent column permeability (1.53 × 10-13m2) and allowed an ultra-fast on-line spe, which dramatically shortens the separation time (< 10 min) and improves the separation efficiency.At high flow velocity (5.0 m l min-1), 50 μl of the extract from eucommia ulmoides leaves can be loaded directly on the cga-mip monoliths and cga with high purity can be obtained with a recovery of 89.01 ± 0.05%.As a conclusion, the resulting rtil-induced approach of preparing mip may be an effective tool in fabricating mip in a low-cost way.Graphical abstract ᅟ.

Journal: Analytical and bioanalytical chemistry (2019)
Publication types: Journal Article
Predicting the Postoperative Addition Power of a Multifocal Intraocular Lens at the Spectacle Plane.

To establish a simple clinical method of predicting addition power achieved with a multifocal intraocular lens (iol).Methods in this prospective cohort study, 41 patients were bilaterally implanted with the bi-flex my multifocal iol (medicontur) with +3.50 diopters (d) near addition power.Monocular defocus curves were plotted for each patient and effective addition power was calculated as the dioptric difference between the distance and near inflection points of the defocus curve.Six biometry formulas (haigis, holladay, srk/t, hill rbf, barrett universal ii, and holladay 2) were used to predict the addition power at the spectacle plane.Results mean effective addition power was 2.60 ± 0.29 d, with significant (p< .01) differences between the prediction methods.Significant differences were found between predicted and effective addition when the holladay, srk/t, hill rbf, and holladay 2 formulas were used.A moderate but significant correlation (r= 0.342,p= .033) was found with the barrett formula, and this was also the method to show the least proportional bias with bland-altman analysis.Conclusions the study demonstrates that the effective addition power can be predicted using the proposed simple clinical method derived using the barrett universal ii formula.The proposed technique may have significant clinical value in screening for patients where ocular biometry may lead to aberrant addition power.[j refract surg.2021;37(5):318-323.].

Journal: Journal of refractive surgery (Thorofare, N.J. : 1995) (2021)
Publication types: Journal Article
Engaging Female Community Health Volunteers (FCHVs) for cardiovascular diseases risk screening in Nepal.

Introduction non-communicable diseases (ncds) have become the leading public health problems worldwide and the cardiovascular diseases (cvds) is one of the major ncds.Female community health volunteers (fchvs) in nepal are the key drivers to implementing frontline health services.We explored the potential for engaging fchvs for cvd risk screening at the community level in nepal.Methods we used multiple approaches (quantitative and qualitative) for data collection.The trained fchvs administered cvd risk screening questionnaire among 491 adults in rural and urban areas and calculated the cvd risk scores.To maintain consistency and quality, a registered medical doctor also, using the same risk scoring chart, independently calculated the cvd risk scores.Kappa statistics and concordance coefficient were used to compare these two sets of risk screening results.Sensitivity and specificity analyses were conducted.Two focus group discussions among the fchvs were conducted to determine their experiences with cvd risk screening and willingness to engage with cvd prevention and control efforts.Results the mean level of agreement between two sets of risk screening results was 94.5% (kappa = 0.77, p<0.05).Sensitivity of fchv screening was 90.3% (95% ci: 0.801-0.964); and the specificity was 97% (95% ci: 0.948, 0.984).Fchvs who participated in the fgds expressed a strong enthusiasm and readiness to using the cvd risk screening tools.Despite their busy workload, all fchvs showed high level of motivation and willingness in using cvd risk screening tools and contribute to the prevention and control efforts of ncds.The fchvs recommended needs for providing additional training and capacity building opportunities.Conclusion we conclude that there is a potential for engaging fchvs to use simple cvd risk screening tools at the community level.The findings are promising, however, further studies engaging larger number of fchvs and larger population would warrant feasibility of such tools within the existing healthcare systems in nepal.

Journal: PloS one (2022)
Publication types: Journal Article