syedzayyan/paper_mill_benchmark huggingface dataset:
Effects of Carvedilol on Blood Pressure, Blood Sugar, and Blood Lipids in Elderly Patients with Refractory Hypertension

Refractory hypertension seriously affects the life safety of patients.To investigate the effect of carvedilol combined with conventional antihypertensive therapy on blood pressure, blood sugar, blood lipids, and cardiovascular and cerebrovascular complications in elderly patients with refractory hypertension, a total of 80 elderly patients with refractory hypertension who were admitted from june 2019 to september 2021 were selected as the retrospective research objects and divided into the observation group and the control group according to the random number table method, 40 cases in each group, and the control group received conventional antihypertensive therapy.On this basis, the observation group was treated with carvedilol, and the effects of blood pressure, blood sugar, blood lipids, and cardiovascular and cerebrovascular complications were analyzed in the two groups.After treatment, the systolic blood pressure, diastolic blood pressure, adrenal medulla (am), and endothelin (et) of the observation group were significantly lower than those of the control group, but the heart rate and no of the observation group were significantly higher than those of the control group.After treatment, the fasting blood glucose, insulin, triacylglycerol (tg), cholesterol (cho), and low-density lipoprotein (ldl-c) of the observation group were significantly higher than those of the control group, but the isi and hdl-c of the observation group were significantly lower than those of the control group.After treatment, the levels of il-1β, il-6, tnf-α, uaer, bun, and scr in the observation group were significantly lower than those in the control group.The tc of the observation group was lower than that of the control group.Combination therapy has a remarkably stable, sustained, and safe antihypertensive effect on patients with refractory hypertension.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
Effects of Acupuncture Combined with Exercise on Expression of Immune Factors in Aging Rats and Its Significance in Antiaging Intervention

With the improvement of people’s living standards, how to maintain health and delay aging to improve the quality of life and achieve longevity has become a hot topic of concern.Objective.To investigate the effect of acupuncture combined with exercise on the expression of immune factors in aging rats and its significance in antiaging intervention.Materials and methods.Forty-three sd rats included 12 rats in the control group, and the remaining rats were injected intraperitoneally with d-galactose 500 mg/kg to prepare a subacute aging rat model.The 24 rats that were successfully modeled were divided into acupuncture exercise groups and exercise groups according to the random number table method, with 12 rats in each group.After the modeling, the comparison group did not do any intervention, the exercise group was given aerobic exercise intervention, and the acupuncture exercise group was given acupuncture combined with exercise intervention.The effect of immune factor expression in rats was compared.Results.The levels of ig m, ig a, and ig g in the acupuncture exercise group were significantly higher than those in the exercise group ( p &lt; 0.05 ).The il-10 content in the acupuncture exercise group was significantly higher than that in the exercise group ( p &lt; 0.05 ) and was significantly reduced in the acupuncture exercise group compared with the comparison group ( p &lt; 0.05 ).The level of il-6 in the acupuncture exercise group was significantly lower than that in the exercise group, and the level of il-6 in the acupuncture exercise group was significantly increased compared with the comparison group ( p &lt; 0.05 ).The c3 and c4 levels in the acupuncture exercise group were significantly higher than those in the exercise group ( p &lt; 0.05 ).The levels of ifn-γ and tnp-α were significantly lower in the acupuncture exercise group than in the exercise group and significantly increased in the acupuncture exercise group compared with the comparison group ( p &lt; 0.05 ).Conclusion.Ling turtle eight method acupuncture combined with exercise promoted the development of immune organ spleen, enhances the body’s immune function and complement system, inhibits the immune inflammatory response and regulates immune balance, reduces the inflammatory response caused by the aging of d-type galactose, and achieves the effect of delaying aging.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
Effect of Transumbilical Single-Port Laparoscopic-Assisted Duhamel Operation on Serum CRP and IL-6 Levels in Children with Hirschsprung’s Disease

To explore the clinical intervention effect of transumbilical single-port laparoscopic-assisted duhamel operation on children with hirschsprung’s disease (hd) and to analyze the effect of treatment on children with serum c-reactive protein (crp) and interleukin-6 (il-6) effects.Methods.Retrospectively select 80 children with hd who underwent surgery in our hospital from may 2017 to may 2020 as the research subjects and they are classified as group a according to the difference of the children’s surgical procedures (receiving transumbilical single-port laparoscopic-assisted duhamel surgery, 40 cases) and group b (receiving conventional laparoscopic surgery, 40 cases), compare the perioperative period (operating time, intraoperative blood loss, surgical posthospitalization, and postoperative gastrointestinal function recovery time), early postoperative complications (perianal dermatitis, urinary retention, enterocolitis, and anastomotic leakage), and late postoperative complications (anastomotic stenosis, dirty stool, recurrence of constipation, and enterocolitis), compare the differences in the levels of crp and il-6 between the two groups of children before and after the operation, and conduct a 1-year follow-up of the two groups of children to compare the long-term defecation status.Results.The surgical time of children in group a, postoperative hospitalization time, and postoperative gastrointestinal function recovery time were significantly shorter than those of group b, and the differences between groups were statistically significant ( p &lt; 0.05 ).A group of patients: the total incidence of postearly complications was 5.00% lower than 22.50% ( p &lt; 0.05 ) in group b ( p &lt; 0.05 ), and the total incidence of previous complications after group a of patients was 10.00% lower than 27.50% of group b ( p &lt; 0.05 ).The two groups of serum crp and il-6 in two groups were not statistically significant ( p &gt; 0.05 ), and the serum crp and il-6 levels of children in group a after surgery were 3 days.It is obviously lower than those in group b, and the differences between groups have statistical significance ( p &lt; 0.05 ).At 1 month after surgery, the average bowel movement time in group a is significantly lower than those of group b ( p &lt; 0.05 ); during the 1–12 months, the difference between the defecation frequency group of the group a and group b did not have statistically significance ( p &gt; 0.05 ).Conclusion.Transumbilical single-port laparoscopic assistant duhamel operation of hd has a good intervention effect, compared to traditional laparoscopic surgery, the operation time, postoperative hospitalization time, and postoperative gastrointestinal function recovery time, and also help to reduce postoperative near-long complications the incidence improves the stress reactions and long-term defecation functions in children.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Effect of Tegretol on Oxidative Stress, Serum Inflammatory Factors, and Left Ventricular Function in AMI Patients after Emergency PCI

To investigate the effects of tegretol on oxidative stress, serum inflammatory factors, and left ventricular function in patients with acute myocardial infarction (ami) after emergency percutaneous coronary intervention (pci), 70 ami patients who received pci in the emergency department of our hospital from january 2021 to december 2021 were collected.The patients in the control group were treated with aspirin, clopidogrel, and heparin sodium during the perioperative period, and the patients in the study group were treated with tegretol.The levels of oxidative stress, serum inflammatory factors, and left ventricular function index were compared between the two groups.The patients in the control group were treated with tt (( 12.00 ± 2.05 ) s), aptt (( 35.50 ± 4.19 ) s), pt (( 16.60 ± 1.58 ) s), tt (( 15.90 ± 2.14 ) s) aptt (( 30.40 ± 3.80 ) s), and pt (( 14.30 ± 1.45 ) s)) and were comparable ( p &gt; 0.05 ), and the difference was statistically significant ( t = 8.210 , 4.600, 7.010, p &lt; 0.001 ).There was no comparable difference in the level of oxidative stress index before treatment ( p &gt; 0.05 ).After treatment, there was significant difference in mda (( 14.53 ± 2.14 ) mmol/l), sod (( 120.45 ± 8.17 ) u/l), mda (( 11.15 ± 2.02 ) mmol/l), and sod (( 129.86 ± 8.55 ) u/l) in the control group ( t = 7.320 , 5.099, p &lt; 0.001 ).The levels of inflammatory factors in patients before treatment were not comparable ( p &gt; 0.05 ).After treatment, there were levels of il-6 (( 3.20 ± 1.05 ) ng/l), crp (( 4.80 ± 1.16 ) mg/l), mpo (( 196.78 ± 21.51 ) mg/l) and tnf-α (( 3.96 ± 0.80 ) pmol/l), il-6 (( 1.95 ± 0.80 ) ng/l), crp (( 3.10 ± 1.02 ) mg/l), mpo (( 163.60 ± 21.10 ) mg/l), and tnf-α in a study group level (( 3.05 ± 0.70 ) pmol/l), with statistically significant difference ( t = 5.187 , 6.028, 6.031, 4.689, p &lt; 0.001 ).Before treatment, there was no comparable difference in the level of left ventricular function index ( p &gt; 0.05 ).After treatment, there was significant difference in lvef ((46.10 ± 2.39) %) and lvdd ((52.06 ± 1.07) mm), lvef ((56.85 ± 2.33) %), and lvdd ((48.75 ± 1.02) mm) in the control group ( t = 17.640 , 21.540, p &lt; 0.001 ).Tegretol as an adjunctive therapy for emergency pci patients with acute myocardial infarction can effectively improve postoperative coagulation function, reduce oxidative stress and inflammatory reaction, and improve cardiac function indicators.It has a positive clinical value.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
Effect of Solitaire FR Stent Thrombectomy Combined with the Suction Thrombus on the Clinical Effect and Prognosis of Acute Middle Cerebral Artery Occlusion

To determine the curative effect and prognosis of solitaire fr stent thrombectomy integrated with the suction thrombus on the treatment of acute middle cerebral artery occlusion (amcao).Based on the treatment, patients suffering from amcao were separated into the solitaire fr group (solitaire fr stent + suction thrombus) and suction group (suction thrombus).Modified thrombolysis in cerebral infarction grading, national institutes of health stroke scale (nihss) score, modified rankin scale score, and safety performance were compared between the two groups.The operation time in the suction group was obviously shorter than the solitaire fr group ( p &lt; 0.05 ).Significant differences were observed in the nihss scores 1 week and 4 weeks after the operation between the solitaire fr group and the suction group ( p &lt; 0.05 ).The nihss scores 1 week and 4 weeks after operation were significantly lower than those before operation ( p &lt; 0.05 ).Nihss scores 1 week after operation did not show obvious difference ( p &gt; 0.05 ).The solitaire fr group showed obvious lower nihss scores than the suction group 4 weeks after surgery ( p &lt; 0.05 ).Statistically obvious difference in cerebral infarction grading of modified thrombolysis between the solitaire fr group and the suction group were observed ( p &lt; 0.05 ).The recanalization rate of the solitaire fr group was obviously higher than the suction group ( p &lt; 0.05 ).The difference in the monthly modified rankin scale score was obvious ( p &lt; 0.05 ).The good prognosis rate of the solitaire fr group was obviously higher than the suction group ( p &lt; 0.05 ).No obvious differences in the incidence of internal bleeding, reocclusion, and 3-month postoperative mortality were observed ( p &gt; 0.05 ).These results showed that the treatment of the solitaire fr stent + suction thrombus in amcao patients has a good thrombus recanalization rate and is helpful in improving the prognosis and safety performance.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;
Effect of Low-Dose Dexmedetomidine Combined with Lumbosacral Plexus Block Guided by Ultrasound Imaging Based on Image Segmentation Algorithm in Fracture Surgery

The aim of this study was to analyze the application of ultrasound-guided low-dose dexmedetomidine combined with lumbosacral plexus block based on artificial intelligence algorithm in the surgical treatment of proximal femoral fractures.104 patients with proximal femoral fractures were divided into 52 cases in the experimental group (ultrasound-guided lumbosacral plexus block combined with dexmedetomidine based on local fitting image segmentation algorithm) and 52 cases in the routine group (endotracheal intubation and inhalation combined with general anesthesia).An image segmentation algorithm based on local fitting was constructed to enhance the ultrasound image.It was found that in the routine group, the heart rate (hr), systolic blood pressure (sbp), and diastolic blood pressure (dbp) at the beginning of intravenous injection of dexmedetomidine, during skin incision, and half an hour after skin incision were significantly lower than those at admission ( p &lt; 0.05 ).The pressing times of patient-controlled intravenous analgesia (pcia) in the conventional group (17.05 ± 6.85 times) were significantly higher than that in the experimental group (8.55 ± 4.12 times), and the difference was statistically significant ( p &lt; 0.05 ).The visual analogue scale (vas) scores at 1, 5, 10, and 15 after operation in the routine group were significantly higher than those in the experimental group ( p &lt; 0.05 ).The number of dizziness, nausea, and vomiting, venous thrombosis of lower limbs, cardiovascular events, and pulmonary infection in the routine group on the 1st, 2nd, and 3rd days after operation were significantly higher than those in the experimental group ( p &lt; 0.05 ).In summary, the ultrasound-guided lumbar plexus-sacral plexus block combined with dexmedetomidine anesthesia based on image segmentation algorithm can effectively maintain the hemodynamic stability of patients, with remarkable analgesic effect and high safety.

Journal: Computational Intelligence and Neuroscience (2022)
Publication types: Research Article;
Direct Detection of Antibiotic Resistance in Chinese Helicobacter pylori Clinical Isolates by Sequencing-Based Approach

The detection of helicobacter pylori mutations that result in antimicrobial resistance can serve as a guideline of antimicrobial therapeutics and probably prevent the failure of clinical treatments.Evaluating the potential of sanger sequencing to identify genetically resistant determinants in helicobacter pylori clinical isolates will be important.Methods.180 cultured strains have been tested using agar dilution for antibiotic susceptibility.Ncbi blast was used to perform genotypic analysis on the sequencing data.Sanger sequencing was evaluated as an alternative method to detect resistant genotypes and susceptibility.Results.By the conventional e-test, resistance to levofloxacin, amoxicillin, metronidazole, and clarithromycin was 67.3%, 15.1%, 96.4%, and 25.5%, respectively.In contrast, tetracycline had no resistance.Resistance to multiple drugs was observed in 8.12% of the strains.The genetic determinants of resistance to cla was 23s r rna, the determinants of resistance to amoxicillin was pbp1, the determinants of resistance to metronidazole was rdx a, and the determinants of resistance to levofloxacin were gyr a and gyr b.However, there was no association of resistance in tetracycline.Conclusion.We found increased rates of metronidazole antibiotic resistance, highlighting the necessity for alternative therapies and periodic evaluation.Sanger sequencing has proved to be highly effective and holds the potential to be implemented in policies catering to local treatments.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Detection and Diagnostic Value of Cytokines in Serum of Children with Acute Viral Encephalitis

Acute viral encephalitis is one of the serious infectious diseases.In order to analyze the diagnostic efficacy of serum procalcitonin (pct), c‐reactive protein (crp), and s100b protein in acute viral encephalitis, a total of 100 children with acute viral encephalitis from july 2019 to december 2021 are selected and included in the viral encephalitis group.The results show that s100b protein model has high specificity and sensitivity and is simple to operate.It provides new ideas and directions for differential diagnosis, improvement, and optimization of relevant clinical diagnosis and treatment schemes and has high clinical value.

Journal: Contrast Media & Molecular Imaging (2022)
Publication types: Research Article;
Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis

Polycystic ovary syndrome (pcos) is the most common endocrine disorder in women of childbearing age.Cryptotanshinone (cry) has been shown to be effective in reversing reproductive disorders, but whether it can be used in the treatment of polycystic ovary syndrome remains unclear.We aimed to explore whether the mechanism of cryptotanshinone (cry) in the treatment of polycystic ovary syndrome (pcos) can be driven via regulating ferroptosis.A rat model of pcos was established by daily injection of human chorionic gonadotropin and insulin for 22 days.An in vitro model of ischemia-reperfusion (ir) of granulosa cells was established.The in vitro and rat models of pcos were subjected to different treatments including ferroptosis activators and inhibitors, cry, and mapk inhibitor.Oxidative stress was evaluated by measuring the activities of sod, mda, and gsh-px.Total body weight and ovarian weight, as well as the levels of lh and the lh to fsh ratio, significantly increased in rats with pcos, compared with controls.The expression of bax was increased in pcos tissues while pgc1α, nfr1, gpx4, catalase p-erk, and bcl-2 were all downregulated.Ferroptosis activator, erastin, had effects similar to those of pcos while the contrary was found with cry and ferroptosis inhibitor treatment groups.In vitro, cry inhibited oxidative stress, mmp, and nf-κb and activated mapk/erk signaling by regulating ferroptosis.Overall, this study indicated that cry protects against pcos-induced damage of the ovarian tissue, via regulating oxidative stress, mmp, inflammation, and apoptosis via regulating ferroptosis.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
CRNDE/ETS1/GPR17 Facilitates the Proliferation, Migration, and Invasion of Glioma

Numerous lnc rnas were found as regulatory factors for occurrence and progression of various tumors, but there is still less research on the role of lnc rnas in malignant progression of glioma.Methods.Bioinformatics analysis analyzed differential genes (degs) in the tcga database.Mtt, flow cytometry, and transwell assays were performed to test the proliferation, apoptosis, migration, and invasion of cells.Q rt-pcr and western blot were conducted to detect rna and protein expressions of each gene, respectively.Chip assay verified the binding relationship between genes.Fish assayed subcellar location of crnde, and xenograft in nude mice was performed for in vivo verification.Results.Crnde was upregulated in glioma cells, and overexpression of crnde facilitated malignant progression of glioma cells.Crnde regulated occurrence and development of glioma through the crnde-ets1-gpr17 axis.Ets1 was proved to target promoter region of gpr17.Overexpression of crnde promoted the binding between ets1 and the promoter region of gpr17, thus, promoting the transcription of gpr17, while silencing of gpr17 inhibited promotion of crnde on proliferation, migration, and invasion of glioma cells.Conclusions.These results demonstrated that crnde regulated gpr17 expression by binding ets1, a transcription factor, thereby affecting glioma development.The results also indicated that crnde could serve as a possible therapeutic target and prognostic biomarker for glioma.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Research Article;
Comparison of Two Surgical Approaches to Supination-External Rotation-Type Ankle Fractures

To compare the clinical outcome and postoperative complications of the treatment of supination-external rotation-type ankle fractures using the posterolateral approach in the prone position and the lateral approach in the supine position.Methods.A retrospective cohort study was conducted in our hospital, including a total of 66 patients ranging from january 2013 to february 2016, regardless of age or sex.All the patients were classified as lauge-hansen supination-external rotation (ser)-type ankle fractures and were assigned to receive an open reduction and internal fixation (orif) through a posterolateral approach performed in the prone position (the “posterolateral approach group,” 32 patients), or a lateral approach performed in the supine position (the “lateral approach group,” 34 patients).In the posterolateral approach group, 5 patients had grade ii fractures, 8 had grade iii fractures, and 19 had grade iv fractures; among whom, 12 underwent fixation of the posterior malleolus with hollow screws and 6 with plates, and none of them had fixation of the distal tibiofibular syndesmosis; in the lateral approach group, 4 patients had grade ii fractures, 12 had grade iii fractures, and 18 had grade iv fractures; among whom, 21 received fixation of the posterior malleolus, and 5 received fixation of the distal tibiofibular syndesmosis.The postoperative complications and ankle scores were recorded.Results.After 1 year of follow-up, in the posterolateral approach group, the incision healed by primary intention and sutures were removed 2 weeks after the operation in all patients; 1 patient had pain in the region posterior to the fibula, presumably peroneal tendon irritation induced by internal fixation, which disappeared after fracture union and fixation device removal; no loosening or breakage of the internal fixation device was reported; the rate of good to excellent results was 96.8% at 12 months.In the lateral approach group: 1 case of wound infection, 1 case of necrosis of lateral skin flap, and 3 cases of lateral skin irritation were reported; the rate of good to excellent results was 94.6% at 12 months.Conclusion.Compared with the traditional lateral approach, the posterolateral approach for the reduction and internal fixation of supination-external rotation-type ankle fractures performed in the prone position has more satisfactory clinical outcomes, with better reduction, more secure fixation, and smaller wound.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;