syedzayyan/paper_mill_benchmark huggingface dataset:
Age-related differences in functional tool-use are due to changes in movement quality and not simply motor slowing.

Age-related declines in fine motor control may impact tool-use and thereby limit functional independence.Most previous research has, however, focused on the effect of aging on gross motor tasks.Few studies have investigated the effects of aging on the strategy or quality of fine motor skills, especially in tool-use, which may better reflect how age impacts complex movement capability.Twenty-two young (ages 19-35) and 18 older adults (ages 58-87) performed a timed upper extremity task using a tool to acquire and transport objects to different locations.Overall task performance was divided into two phases based on 3-d position of the tool: a gross motor phase (object transport) and a fine motor phase (object acquisition).Overall, older adults took longer to complete the task.A linear model indicated that this was due to the duration of the fine motor phase more so than the gross motor phase.To identify age-related differences in the quality of the fine motor phase, we fit three-dimensional ellipsoids to individual data and the calculated the ellipsoid volume.Results demonstrated a significant volume-by-age interaction, whereby increased ellipsoid volume (space the tool occupied) related to increased mean dwell time for the older adult group only; younger adults did not demonstrate this relationship.Additionally, older adults with longer movement times during the fine motor phase also had lower cognitive scores.No age-related differences were observed for the gross motor phase, suggesting that age-related declines in tool-use may be due to changes in fine motor control and cognitive status.

Journal: Experimental brain research (2021)
Publication types: Journal Article
Yes-associated protein expression in paired primary and local recurrent breast cancer and its clinical significance.

Yes-associated protein (yap) protein acts as tumorigenic factor in many solid tumors, but the situation in breast cancer is under debate.Here, we would analyze its status in breast cancer.Yap expression in the 110 primary breast cancer and their paired local recurrent tumors was investigated.Clinicopathologic data for age, histologic grading, hormone status, lymph nodes and her2 status were also gathered and analyzed.46.4% (51/110) primary breast cancer tissues were positive for total yap expression which was significantly higher than that in the recurrent tissues (10.9%; p < 0.05).The expression of total yap protein in the primary breast cancer tissues was positively associated with the tumor size, especially in triple negative breast cancer (tnbc) subtype (p < 0.05).Higher total or nuclear yap expression in the primary tumor was correlated with poor disease-free survival among patients with tnbc (p < 0.05).In the multivariate models, nuclear yap expression was an independently prognostic factor in tnbc.High total or nuclear yap expression predicts poor prognosis among patients with tnbc.It might be a therapeutic target for tnbc in the future.

Journal: Current problems in cancer (2019)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Chronic Aflatoxin Exposure and Cognitive and Language Development in Young Children of Bangladesh: A Longitudinal Study.

Aflatoxin can cross the blood-brain barrier, damage brain tissues, and have the potential to harm the development of the human brain.Although dietary aflatoxin exposure is common in children, there is a paucity of data on aflatoxin exposure and child developmental outcomes.The child's cognitive, motor, and language functions were assessed using the bayley scales of infant and toddler development-iii or bsid-iii at the same time points.Association between exposure to aflatoxin and subtests of bsid-iii were examined using mixed-effect linear regression.Aflatoxin assays were performed on 194, 167, and 163 children at 15, 24, and 36 months of age, and chronic aflatoxin exposure was detected in 20.6%, 16.8%, and 60.7% of children, respectively.Multi-variable analyses showed that aflatoxin exposure was independently related to the children's cognitive score (β: -0.69; 95% ci: -1.36, -0.02), receptive language score (β: -0.90; 95% ci: -1.62, -0.17), and expressive language score (β: -1.01; 95% ci: -1.96, -0.05).We did not observe any association between exposure to aflatoxin and the motor function of children.Chronic exposure to aflatoxin exposure was linked to reduced cognitive, expressive, and receptive language scores of the study children.Further research is needed in a different setting to confirm this novel finding.

Journal: Toxins (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Spatial delivery of immune cues to lymph nodes to define therapeutic outcomes in cancer vaccination.

Recently approved cancer immunotherapies - including car-t cells and cancer vaccination, - show great promise.However, these technologies are hindered by the complexity and cost of isolating and engineering patient cellsex vivo.Lymph nodes (lns) are key tissues that integrate immune signals to coordinate adaptive immunity.Directly controlling the signals and local environment in lns could enable potent and safe immunotherapies without cell isolation, engineering, and reinfusion.Here we employ intra-ln (i.ln.) injection of immune signal-loaded biomaterial depots to directly control cancer vaccine deposition, revealing how the combination and geographic distribution of signals in and between lns impact anti-tumor response.We show in healthy and diseased mice that relative proximity of antigen and adjuvant in lns - and to tumors - defines unique local and systemic characteristics of innate and adaptive response.These factors ultimately control survival in mouse models of lymphoma and melanoma.Of note, with appropriate geographic signal distributions, a singlei.ln.vaccine treatment confers near-complete survival to tumor challenge and re-challenge 100 days later, without additional treatments.These data inform design criteria for immunotherapies that leverage biomaterials for loco-regional ln therapy to generate responses that are systemic and specific, without systemically exposing patients to potent or immunotoxic drugs.

Journal: Biomaterials science (2022)
Publication types: Journal Article
Phototransduction in Anuran Green Rods: Origins of Extra-Sensitivity.

Green rods (grs) represent a unique type of photoreceptor to be found in the retinas of anuran amphibians.These cells harbor a cone-specific blue-sensitive visual pigment but exhibit morphology of the outer segment typical for classic red rods (rrs), which makes them a perspective model object for studying cone-rod transmutation.In the present study, we performed detailed electrophysiological examination of the light sensitivity, response kinetics and parameters of discrete and continuous dark noise in grs of the two anuran species: cane toad and marsh frog.Our results confirm that anuran grs are highly specialized nocturnal vision receptors.Moreover, their rate of phototransduction quenching appeared to be about two-times slower than in rrs, which makes them even more efficient single photon detectors.The operating intensity ranges for two rod types widely overlap supposedly allowing amphibians to discriminate colors in the scotopic region.Unexpectedly for typical cone pigments but in line with some previous reports, the spontaneous isomerization rate of the gr visual pigment was found to be the same as for rhodopsin of rrs.Thus, our results expand the knowledge on anuran grs and show that these are even more specialized single photon catchers than rrs, which allows us to assign them a status of "super-rods".

Journal: International journal of molecular sciences (2021)
Publication types: Journal Article
The Role of Simultaneous Integrated Boost in Locally Advanced Rectal Cancer Patients with Positive Lateral Pelvic Lymph Nodes.

S: between 11 to 14% of patients with locally advanced rectal cancer (larc) have positive lateral pelvic lymph nodes (lpln) at diagnosis, related to a worse prognosis with a 5-year survival rate between 30 to 40%.The best treatment choice for this group of patients is still a challenge.The optimal radiotherapy (rt) dose for lpln patients has been investigated.Methods: we retrospectively collected data from larc patients with lpln at the primary staging mri, treated in our center from march 2003 to december 2020.Patients underwent a neoadjuvant concomitant chemo-radiotherapy (crt) treatment on the primary tumor (t), mesorectum, and pelvic nodes, associated with a fluoride-based chemotherapy.The total reached dose was 45 gy at 1.8 gy/fr on the elective sites and 55 gy at 2.2 gy/fr on the disease and mesorectum.Patients were divided in two groups based on whether they received a simultaneous integrated rt boost on the lpln or not.Overall survival (os), disease free survival (dfs), metastasis free survival (mfs), and local control (lc) were evaluated in the whole group and then compared between the two groups.Results: a total of 176 patients were evaluated: 82 were included in the rt boost group and 94 in the non-rt boost group.The median follow-up period was 57.8 months.All the clinical endpoint (os, dfs, mfs, lc), resulted were affected by the simultaneous integrated boost on lpln with a survival rate of 84.7%, 79.5%, 84.1%, and 92%, respectively, in the entire population.From the comparison of the two groups, there was a statistical significance towards the rt boost group with a p < 0.006, 0.030, 0.042, 0.026, respectively.Conclusions: concomitant radiotherapy boost on positive lpln has shown to be beneficial on the survival outcomes (os, dfs, mfr, and lc) in patients with larc and lpln.This analysis demonstrates that a higher dose of radiotherapy on positive pelvic lymph nodes led not only to a higher local control but also to a better survival rate.These results, if validated by future prospective studies, can bring a valid alternative to the surgery dissection without the important side effects and permanent disabilities observed during the years.

Journal: Cancers (2022)
Publication types: Journal Article
HPV Vaccination Intentions of Female Students in Chinese Universities: A Systematic Literature Review and Meta-Analysis.

To systematically evaluate the human papillomavirus (hpv) vaccination intentions among female university students in china and establish a basis for improving hpv vaccination coverage.Methods we searched cnki, ebsco, jstor, mesh or emtree, weipu information chinese journal service platform, wanfang data, china biomedical literature database, pub med, embase, web for the web of science, and the cochrane library to identify peer-reviewed published research on intentions by female college students in china to receive the hpv vaccination.Results a preliminary search of 408 papers resulted in the inclusion of 12 studies, all cross-sectional, of moderate or high quality, with a sample size of 12,600.The hpv vaccination intention rate among chinese female university students was 16.67% (95% ci: 12.38% to 21.24%).The vaccination intention rates of medical students, non-medical students, and tibetan students were 30.37% (95% ci: 28.80-34.12%), 15.53% (95% ci: 11.2-20.22%), and 14.12% (95 % ci: 10.59-18.04%), respectively.The vaccination intention rates of the participants with parental education of junior high school and below, high school, and bachelor's degree and above were 15.36% (95 % ci: 11.59 to 17.54%), 17.18% (95 % ci: 12.33% to 19.61%), and 19.81% (95 % ci: 15.61% to 22.25%), respectively.The intention rates of vaccination among residents of first-tier, second-tier, and third-tier cities were 17.64% (95% ci: 12.76-21.63%), 15.39% (95% ci: 11.74-19.82%), and 13.87% (95% ci: 9.36-15.65%), respectively.The results of the meta-analysis were relatively stable with little publication bias.Conclusion the intention rate of hpv vaccination among female university students in china is low and varies among different populations.There is a need to increase hpv vaccination promotion efforts to improve the intention of female university students to receive the vaccine.

Journal: International journal of environmental research and public health (2022)
Publication types: Journal Article;Meta-Analysis;Review;Systematic Review
A cancer cell-specific benzoxadiazole-based fluorescent probe for hydrogen sulfide detection in mitochondria.

The present work describes the design and biological applications of a novel colorimetric and fluorescence turn-on probe for hydrosulfide detection.The probe was designed to introduce hemicyanine as the fluorescent skeleton and 7-nitro-1,2,3-benzoxadiazole as the recognition site.The optical properties and responses of the probe towards hs-, anions and some biothiols indicate an impressively high selectivity of the probe towards hs-such that it can be effectively used as an indicator for monitoring the level of hs-in living cells.In biological experiments using the probe, the h2s levels are found to be higher in cancer cells than in normal cells.In addition, the probe is shown to specifically and rapidly detect endogenous h2s, which is produced primarily in the mitochondria of cancer cells, as demonstrated by a co-localization experiment using specific trackers for the detection of cellular organelles in pharmacological inhibition or stimulation studies, without any significant cytotoxic effects.Thus, the results of the chemical and biological experiments described herein demonstrate the potential of this novel probe to specifically, safely, and rapidly detect h2s to distinguish cancer cells from normal cells by targeting it specifically in mitochondria.

Journal: Dalton transactions (Cambridge, England : 2003) (2021)
Publication types: Journal Article
Use of a carbonic anhydrase Ca17a knockout to investigate mechanisms of ion uptake in zebrafish (Danio rerio).

In fishes, branchial cytosolic carbonic anhydrase (ca) plays an important role in ion and acid-base regulation.The ca17a isoform in zebrafish (danio rerio) is expressed abundantly in na+-absorbing/h+-secreting h+-atpase-rich (hr) cells.The present study aimed to identify the role of ca17a in ion and acid-base regulation across life stages using crispr/cas9 gene editing.However, in preliminary experiments, we established thatca17aknockout is lethal withca17a-/-mutants exhibiting a significant decrease in survival beginning at ∼12 days postfertilization (dpf) and with no individuals surviving past 19 dpf.Based on these findings, we hypothesized thatca17a-/-mutants would display alterations in ion and acid-base balance and that these physiological disturbances might underlie their early demise.Na+uptake rates were significantly increased by up to 300% in homozygous mutants compared with wild-type individuals at 4 and 9 dpf; however, whole body na+content remained constant.While cl-uptake was significantly reduced inca17a-/-mutants, cl-content was unaffected.Reduction of ca activity by ca17a morpholino knockdown or ethoxzolamide treatments similarly reduced cl-uptake, implicating ca17a in the mechanism of cl-uptake by larval zebrafish.H+secretion, o2consumption, co2excretion, and ammonia excretion were generally unaltered inca17a-/-mutants.In conclusion, while the loss of ca17a caused marked changes in ion uptake rates, providing strong evidence for a ca17a-dependent cl-uptake mechanism, the underlying causes of the lethality of this mutation in zebrafish remain unclear.

Journal: American journal of physiology. Regulatory, integrative and comparative physiology (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Hyper-Sensitivity to Pitch and Poorer Prosody Processing in Adults With Autism: An ERP Study.

Individuals with autism typically experience a range of symptoms, including abnormal sensory sensitivities.However, there are conflicting reports on the sensory profiles that characterize the sensory experience in autism that often depend on the type of stimulus.Here, we examine early auditory processing to simple changes in pitch and later auditory processing of more complex emotional utterances.We measured electroencephalography in 24 adults with autism and 28 controls.First, tones (1046.5hz/c6, 1108.7hz/c#6, or 1244.5hz/d#6) were repeated three times or nine times before the pitch changed.Second, utterances of delight or frustration were repeated three or six times before the emotion changed.In response to the simple pitched tones, the autism group exhibited larger mismatch negativity (mmn) after nine standards compared to controls and produced greater trial-to-trial variability (ttv).In response to the prosodic utterances, the autism group showed smaller p3 responses when delight changed to frustration compared to controls.There was no significant correlation between erps to pitch and erps to prosody.Together, this suggests that early auditory processing is hyper-sensitive in autism whereas later processing of prosodic information is hypo-sensitive.The impact the different sensory profiles have on perceptual experience in autism may be key to identifying behavioral treatments to reduce symptoms.

Journal: Frontiers in psychiatry (2022)
Publication types: Journal Article
Structure and age-dependent development of the turkey liver: a comparative study of a highly selected meat-type and a wild-type turkey line.

In this study the macroscopic and microscopic structure of the liver of a fast growing, meat-type turkey line (british united turkeys but big 6, n=25) and a wild-type turkey line (wild canadian turkey, n=48) were compared at the age of 4, 8, 12, 16, and 20 wk.Because the growth plates of long bones were still detectable in the 20-week-old wild-type turkeys, indicating immaturity, a group of 8 wild-type turkeys at the age of 24 wk was included in the original scope of the study.Over the term of the study, the body and liver weights of birds from the meat-type turkey line increased at a faster rate than those of the wild-type turkey line.However, the relative liver weight of the meat-type turkeys declined (from 2.7 to 0.9%) to a greater extent than that of the wild-type turkeys (from 2.8 to 1.9%), suggesting a mismatch in development between muscle weights and liver weights of the meat-type turkeys.Signs of high levels of fat storage in the liver were detected in both lines but were greater in the wild-type turkey line, suggesting a better feed conversion by the extreme-genotype birds i.e., meat-type birds.For the first time, this study presents morphologic data on the structure and arrangement of the lymphatic tissue within the healthy turkey liver, describing two different types of lymphatic aggregations within the liver parenchyma, i.e., aggregations with and without fibrous capsules.Despite differences during development, both adult meat-type and adult wild-type turkeys had similar numbers of lymphatic aggregations.

Journal: Poultry science (2016)
Publication types: Comparative Study;Journal Article
Aquatic life criteria & human health ambient water quality criteria derivations and probabilistic risk assessments of 7 benzenes in China.

The benzenes have attracted worldwide attention due to their high biological toxicity in the environment.In this study, using species sensitivity distribution method to derive the aquatic life criteria of 7 benzenes (carbazole, 1,3-dichlorobenzene, 1,4-dichlorobenzene, 1,2,4-trichlorobenzene, phenol, 2,4-dichlorophenol and nitrobenzene), then risk quotient method (rq), potentially affected fraction (paf) method and joint probability curve (jpc) method were applied for multilevel ecological risk assessment for 7 benzenes in tai lake basin.In addition, the human health ambient water quality criteria (awqc) of 7 benzenes were derived according to usepa guidelines, and the probability distributions of human health awqc for 7 benzenes in china were simulated by monte carlo simulation combined with crystal ball software.Finally, the health risks of 7 benzenes in tai lake were assessed by rq method assisted by monte carlo simulation.The results showed that nitrobenzene had the maximum aquatic life criteria value, followed by phenol, chlorobenzenes, 2,4-dichlorophenol and carbazole.All recommended human health awqc values of 7 benzenes were found at a position of 27th-55th percentiles in the output criteria distributions, indicating that recommended national human health awqc values could provide effective protection for most of the population in china.Furthermore, the consumption of aquatic products was found to be the most influential parameter of human health awqc for benzenes with higher kow values.The risk assessments showed that noncarcinogenic 2,4-dichlorophenol had potential ecological risk, carcinogenic carbazole and 1,2,4-trichlorobenzene had significant human health risk in tai lake.

Journal: Chemosphere (2021)
Publication types: Journal Article
A phosphine-based redox method for direct conjugation of disulfides.

Technologies for cysteine disulfide detection and conjugation are pivotal to understanding protein functions and developing disulfide-derived therapeutic agents.Currently, disulfide modification requires reductive cleavage prior to functionalization, posing challenges to differentiating disulfides from free thiols.We describe herein redox-assisted disulfide direct conjugation (rddc) as a new method to enable disulfide rebridging without cross-reacting with free thiols.

Journal: Chemical communications (Cambridge, England) (2022)
Publication types: Journal Article
Joint Inversion of GNSS and GRACE for Terrestrial Water Storage Change in California.

Global navigation satellite system (gnss) vertical displacements measuring the elastic response of earth's crust to changes in hydrologic mass have been used to produce terrestrial water storage change (∆tws) estimates for studying both annual ∆tws as well as multi-year trends.However, these estimates require a high observation station density and minimal contamination by nonhydrologic deformation sources.The gravity recovery and climate experiment (grace) is another satellite-based measurement system that can be used to measure regional tws fluctuations.The satellites provide highly accurate ∆tws estimates with global coverage but have a low spatial resolution of ∼400 km.Here, we put forward the mathematical framework for a joint inversion of gnss vertical displacement time series with grace ∆tws to produce more accurate spatiotemporal maps of ∆tws, accounting for the observation errors, data gaps, and nonhydrologic signals.We aim to utilize the regional sensitivity to ∆tws provided by grace mascon solutions with higher spatial resolution provided by gnss observations.Our approach utilizes a continuous wavelet transform to decompose signals into their building blocks and separately invert for long-term and short-term mass variations.This allows us to preserve trends, annual, interannual, and multi-year changes in tws that were previously challenging to capture by satellite-based measurement systems or hydrological models, alone.We focus our study in california, usa, which has a dense gnss network and where recurrent, intense droughts put pressure on freshwater supplies.We highlight the advantages of our joint inversion results for a tectonically active study region by comparing them against inversion results that use only gnss vertical deformation as well as with maps of ∆tws from hydrological models and other grace solutions.We find that our joint inversion framework results in a solution that is regionally consistent with the grace ∆tws solutions at different temporal scales but has an increased spatial resolution that allows us to differentiate between regions of high and low mass change better than using grace alone.

Journal: Journal of geophysical research. Solid earth (2022)
Publication types: Journal Article