syedzayyan/paper_mill_benchmark huggingface dataset:
Exosomes function as nanoparticles to transfer miR-199a-3p to reverse chemoresistance to cisplatin in hepatocellular carcinoma

Hepatocellular carcinoma (hcc) is a frequently seen malignant tumor globally.The occurrence of cisplatin (ddp) resistance is one of the main reasons for the high mortality of hcc patients.Therefore, it is of great theoretical significance and application value to explore the mechanism of chemotherapy resistance.Drug resistance can be modulated by exosomes containing m rnas, micro rnas (mi rnas) and other non-coding rna (nc rnas).Exosomal mi r-199a-3p (exo-mi r-199a-3p) was subjected to extraction and verification.Whether exo-mi r-199a-3p could make hcc cells sensitive to ddp in vitro was verified via flow cytometry, cell counting kit-8 (cck-8) assay, immunofluorescence assay and transwell assay.Intravenous injection of exo-mi r-199a-3p and intraperitoneal injection of ddp were carried out in vivo.Moreover, the possible targets of mi r-199a-3p were screened through bioinformatics analysis, which were ascertained by western blotting (wb).Then, mi r-199a-3p levels in human normal liver epithelial cell line hl-7702 and hcc cell lines hu h7 and hu h7/ddp were elevated in a concentration-dependent manner.Exo-mi r-199a-3p has abilities to adjust underlying targets and conjugate cells, to repress cells to invade, stimulate their apoptosis and abate their ability.Additionally, the caudal injection of exo-mi r-199a-3p reversed the chemoresistance of tumors and slowed down their growth in the body owing to the up-regulation of mi r-199a-3p and down-regulation of underlying target proteins in tumors.Finally, exo-mi r-199a-3p was found to overturn the hcc’s resistance to ddp, and it may function in ddp-refractory hcc therapy as an underlying option in the future.

Journal: Bioscience Reports (2020)
Publication types: Research Article;
Long non-coding RNA TPT1-AS1 promotes cell growth and metastasis in cervical cancer via acting AS a sponge for miR-324-5p

Background increasing studies confirmed that abnormal lnc rnas expression play a critical role in cervical cancer (cc) development and progression.Lnc rna tpt1-as1, a novel lnc rna, its role and underlying mechanisms involved in cc remain largely unknown.Methods colony formation, ed u and transwell assays were used to determine colony formation, proliferation, migration and invasion in vitro.The subcutaneous tumor model and tail vein injection lung metastasis model were performed to check tumor growth and metastasis in vivo.Luciferase activity and rip experiment were carried out to determine the interaction between mi r-324-5p and tpt1-as1.Results we demonstrated for the first time that tpt1-as1 expression was up-regulated in cc tissues and cell lines.High tpt1-as1 was significantly correlated with adverse prognostic characteristics and poor survival.Tpt1-as1 overexpression and knockdown experiments revealed that tpt1-as1 promoted cell colony formation, proliferation, migration, invasion and emt progression of cc cells in vitro and in vivo.The underlying mechanism indicated that tpt1-as1 functioned as an endogenous sponge for mi r-324-5p in cc cells.Gain- and loss- experiment confirmed that mi r-324-5p inhibited cell colony formation, proliferation, migration, invasion and emt progression of cc cells, and mediated the biological effects of tpt1-as1.Further investigations confirmed that sp1 was a direct target of mi r-324-5p and mediated the effects of tpt1-as1 and mi r-324-5p in cc.Conclusions we demonstrated for the first time that tpt1-as1 as an oncogenic lnc rna in cc progression and as a potential target for cc cure.

Journal: Journal of Experimental & Clinical Cancer Research: (CR) (2018)
Publication types: Research Article;
miR-200c, a tumor suppressor that modulate the expression of cancer stem cells markers and epithelial-mesenchymal transition in colorectal cancer

Colorectal cancer (crc) is the most frequently diagnosed cancer and the most common gastrointestinal cancer worldwide.Due to the presence of populations of cancer stem cells (cscs) that cause recurrence, the possibility of cancer treatment is very low.The aim of current study was to evaluate the inhibitory role of mi r‐200c on emt, cscs markers and β‐catenin in hct‐116 and sw48 cell lines.The expression of mi r‐200c, emt‐related genes, cscs markers, and β‐catenin were quantified by q rt‐pcr.Further, expression of β‐catenin and emt‐related proteins, and migration were analyzed by western blot, and migration assay kit, respectively.Spheroid formation assay was used to enrich colorectal cscs from colorectal cancer cell lines.Lna‐anti‐mi r‐200c suppressed the endogenous mi r‐200c in transfected cells compared with the control.Q rt pcr and western blot analysis of lna‐anti‐mi r‐200c transfected cells revealed a considerable increase in cscs markers, vimentin, zeb‐1, n‐cadherin, and β‐catenin expression, with a concomitant reduction in e‐cadherin expression level.Migration and sphere forming ability of hct‐116 and sw48 cells increased in transfected cells.The results of current study revealed that downregulation of mi r‐200c may be an important factor for the overexpression of cscs markers and emt related genes via β‐catenin upregulation in crc.

Journal: Journal of Cellular Biochemistry (2018)
Publication types: Research Article;
Lentiviral Vector-Mediated SHC3 Silencing Exacerbates Oxidative Stress Injury in Nigral Dopamine Neurons by Regulating the PI3K-AKT-FoxO Signaling Pathway in Rats with Parkinson's Disease

Parkinson’s disease (pd) is a prevalent disease that leads to motor and cognitive disabilities, and oxidative stress (os) injury was found to be related to the etiology of pd.Increasing evidence has shown that shc3 is aberrantly expressed in neurons.The current study examines the involvement of shc3 silencing in os injury in the nigral dopamine neurons in rats with pd via the pi3k-akt-fox o signaling pathway.Methods: to study the mechanisms and functions of shc3 silencing in pd at the tissue level, 170 rats were selected, and a lentivirus-based packaging system was designed to silence shc3 expression in rats.Furthermore, pc12 cells were selected for in vitro experimentation.To evaluate the effect of shc3 silencing in nigral dopamine neuronal growth, an mtt assay, propidium iodide (pi) single staining and annexin v-pi double staining were performed to detect cell viability, cell cycle progression and cell apoptosis, respectively.Results: shc3 sh rna led to decreased sod and mda levels and enhanced gsh activity, indicating that shc3 silencing leads to motor retardation.Shc3 silencing repressed the extent of akt and fox o phosphorylation, thereby inhibiting the pi3k-akt-fox o signaling pathway.Furthermore, in cell experiments, shc3 silencing suppressed pc12 cell proliferation and cell cycle progression, whereas it enhanced cell apoptosis.Conclusion: the current study provides evidence suggesting that shc3 silencing may aggravate os injury in nigral dopamine neurons via downregulation of the pi3k-akt-fox o signaling pathway in pd rats.

Journal: Cellular Physiology and Biochemistry (2018)
Publication types: Research Article;
Effects of the Long Non-Coding RNA HOST2 On the Proliferation, Migration, Invasion and Apoptosis of Human Osteosarcoma Cells

This study aimed to explore the effects of the long non-coding rna host2 (lnc-host2) on the proliferation, migration, invasion and apoptosis of osteosarcoma cells.Methods: osteosarcoma tissues and adjacent normal tissues from 52 patients were selected.Human osteosarcoma cell lines (sa os2, hos, u2os and mg-63) were collected and cultured; mg-63 cells had the highest lnc-host2 expression and thus were used in subsequent experiments.Then, mg-63 cells were transfected and divided into the blank (no transfection), si-con (transfected with negative control si rna) and si-lnc-host2 (transfected with small interference lnc-host2 si rna) groups.Quantitative real-time polymerase chain reaction (q rt-pcr) was used to determine the expression of lnc-host2 in primary tissues and cells.Cell growth was detected using the cck-8 and colony formation assays.Cell doubling time was detected.Cell migration and invasion were observed using the scratch test and transwell assays.Cell apoptosis and cell cycle progression of osteosarcoma cells were detected using flow cytometry with annexin v/pi double staining and pi staining, respectively.Results: the level of lnc-host2 expression in the si-lnc-host2 group was significantly decreased compared to that in the blank and si-con groups.The od values in the si-lnc-host2 group were significantly lower than those in the blank and si-con groups.Compared to the blank and si-con groups, the si-lnc-host2 group presented significant decreases in the colony number and healing rates after scratching.The number of invasive cells in the si-lnc-host2 group was significantly less than that in the blank and si-con groups.In the si-lnc-host2 group, the cell cycle was mainly halted in the g1 phase, and the apoptosis rate and doubling time in this group were significantly higher than those in the blank group and si-con group.Conclusions: inhibition of lnc-host2 could suppress the proliferation, migration, and invasion and promote the apoptosis of osteosarcoma cells.

Journal: Cellular Physiology and Biochemistry (2017)
Publication types: Research Article;
Fructose Diet-Induced Skin Collagen Abnormalities Are Prevented by Lipoic Acid

Nonenzymatic glycation of proteins, leading to chemical modification and cross‐linking are of importance in the pathology of diabetic complications.we studied the effect of α ‐lipoic acid (la) on the content and characteristics of the protein collagen from skin of high‐fructose fed rats.The rats were divided into 4 groups of 6 each.Two groups of rats were fed with a high fructose diet (60 g/100 g diet) and administered either la (35 mg/kg b.w., i.p) (fru+la) or 0.2 ml vehicle (saline) (fru) for 45 days.The other 2 groups were fed with control diet containing starch (60 g/100 g diet) and administered either saline (con) or lipoic acid (con+la).The rats were maintained for 45 days and then sacrificed.Plasma glucose, insulin, fructosamine, protein glycation, and blood glycated hemoglobin (hb a 1 c) were measured.Collagen was isolated from skin and the physicochemical properties of collagen were studied.Fructose administration caused accumulation of collagen in skin.Extensive cross‐linking was evidenced by enhanced glycation and age‐linked fluorescence.Increased peroxidation and changes in physicochemical properties such as shrinkage temperature, aldehyde content, solubililty pattern, susceptibility to denaturing agents were observed in fructose‐fed rats.Sds gel pattern of collagen from these rats showed elevated β component of type i collagen.These changes were alleviated by the simultaneous administration of la.Administration of la to fructose‐fed rats had a positive influence on both quantitative and qualitative properties of collagen.The results suggest a mechanism for the ability of la to delay diabetic complications.

Journal: Journal of Diabetes Research (2004)
Publication types: Research Article;
Working conditions and effective supervision: Does it matter for engineering faculty job satisfaction

The purpose of this study is to examine the impact of supervisor support and working conditions on job satisfaction among teaching faculty in private engineering colleges.The study on job satisfaction has greater importance with the passage of time because of its nature and impact on the faculty and society.The researcher administered a standardized questionnaire on 180 individual respondents to collect the data.Respondents filled the items manually with their characteristics: age, gender, qualification, work experience, and their job position to check their level of significance with the job satisfaction.Descriptive statistics, correlation, and regression analysis were used for the data analysis.Based on the statistical findings, supervisor support and working conditions have a significant positive relationship with job satisfaction.These findings extend the theoretical understanding of supervisor support and work environment which enhance the faculty job satisfaction.Results also urge colleges and higher educational institutions to understand and to emphasize the importance of the work environment, which has an impact on faculty job satisfaction.

Journal: International Journal of Electrical Engineering & Education (2019)
Publication types: Article in Press;Research Article;
Development of triple streaming (HD-SDI, EX-SDI, analog signals) hybrid CCTV camera

This study proposes the developing and implementing of the high-definition level triple streaming hybrid security camera that can output three types of video-signals, which are high-definition serial digital interface, extended serial digital interface, and analog signals.In this study, to develop the high-definition level triple streaming hybrid security camera the hardware and firmware software was designed and implemented.The hardware was implemented using image sensors mn34229pl from panasonic, main processors en778/en331 from eyenix, auto iris functions ka909a from fairchild, and zoom/focus controls a3901sejtr-t from microsystems inc.In addition, to evaluate the performance test of the developed camera in this study, we requested for the performance evaluation at the broadcasting and communication convergence testing by the department of telecommunication technology association, korea.The results of the performance tests indicated that the performances of the product developed in this study were found to be excellent than the other commercial products with regard to the signal-to-noise ratio, the minimum intensity of illumination, and power consumption.The product designed in this study is expected to be widely utilized in the high-resolution security camera market.

Journal: International Journal of Electrical Engineering & Education (2019)
Publication types: Article in Press;Research Article;
A study on the development of an autonomous electrical safety management service using an IoT-based smart outlet

At present, individual households within a multi-family residential are at susceptible to electrical accidents.As if in a blind spot, they cannot be covered under electrical safety management.This paper presents issues about an autonomous electrical safety management service which uses smart outlets to ensure electrical safety for individual households.Risk factors surrounding electrical incidents occurring in a mashup tech-based environment were analyzed and a smart outlet equipped with a prediction technique was developed.In addition to this, an autonomous electrical safety management service scenario was developed to handle mechanical failures in autonomous electrical safety devices which run on io t-based smart outlets.By utilizing such an autonomous electrical safety management service model, technology for electrical safety security was developed for individual households or each residential unit in multi-dwelling unit.The correlation among voltage, current, and zero phase current was analyzed to identify the risk factors of electrical accidents and to predict accidents.In order to detect arc faults, a new prediction technique was developed.The technique is based on the mashup technology after analyzing waveform and fft derived from the correlation among voltage, current, and zero phase current.In particular, the techniques applied to analyze nonlinear models such as an arc fault show the diversity in the application areas of traditional fast fourier transform (fft) algorithms.Furthermore, by using such a technique for electrical accident prediction, an autonomous electrical safety management model was developed.Thus, a measure to ensure micro-grid electrical safety was presented.There is the hope that this development of the management technique for individual households in connection with the fourth industrial revolution would contribute to electrical safety.

Journal: International Journal of Electrical Engineering & Education (2019)
Publication types: Article in Press;Research Article;
Analysis on the problems faced by non-general language talent reserve project

Under the current economic development situation, “one belt and one road” is a development strategy highly emphasized by the countries along the “one belt and one road” route including chinese government.This strategy involves the strength development and enhancement of multiple hardwares, including economic cooperation, international trade, and infrastructure construction.Meanwhile, it also involves the enhancement of software strength, including talent cultivation and reserve, culture inheritance and advocation, policy formulation and implementation.Wherein talent reserve is the important basis, especially under the background of great integration of economy and culture, the non-general language talent cultivation and reserve project is crucial.This paper analyzes the problems existed in the non-general language talent cultivation and reserve under the background of “one belt and one road” and puts forward countermeasures.

Journal: International Journal of Electrical Engineering & Education (2019)
Publication types: Article in Press;Research Article;
Risk assessment index system for equipment maintenance project of civil military integration

In view of the risks of equipment maintenance in the military and civilian integration, by analyzing the experience of foreign military and civilian integration, the relevant cases are analyzed, combined with modern management theory of agency theory, entrustment theory, risk management theory, and virtual organization theory.This paper constructs the risk assessment index system of the military and civilian integration equipment maintenance project on the basis of fully considering the particularity of the service and uses the factor clustering, the comprehensive weighting method, and the double base point evaluation method to carry out the evaluation.The validity and practicability of the evaluation are verified by examples, which can provide some reference for the risk assessment of equipment maintenance project in the integration of military and civilian.

Journal: International Journal of Electrical Engineering & Education (2019)
Publication types: Article in Press;Research Article;
Energy-efficient routing protocol based on tree link topology in wireless sensor networks

Wireless sensor networks have been widely used in target monitoring application.It is often assumed in a typical target coverage algorithm that the environment is known, and each target is covered by only one node.However, these algorithms are not scalable.In fact, one target may need to be covered by more than one node, which is the k-coverage problem.Aiming at the application of target monitoring in wireless sensor network, a k-coverage model— kernal matrix based genetic algorithm (kmga) based on the genetic algorithm—is proposed.The kmga model ensures that each target is covered by multiple sensors simultaneously.First, multiple covers are generated as much as possible through genetic algorithm, and then, while coverage requirements are ensured, coverage between covers is switched based on remaining energies of nodes so as to extend the network life.Experimental data show that the proposed kmga model can effectively extend the network lifetime and ensure coverage rate.

Journal: International Journal of Electrical Engineering & Education (2019)
Publication types: Article in Press;Research Article;
Control schemes for micro-grid application—A simulation and experimental approach for Electrical Engineering Project

The knowledge gain of micro-grid operation control schemes for micro-grid application is important for graduate and undergraduate students.In this paper, the issues on photovoltaic integrated to grid system and the control schemes are discussed from basics and the level of students’ understanding.Initially the basic photovoltaic modeling, maximum power point tracking techniques, and grid synchronization are presented.For grid synchronization, the mostly used synchronous reference frame-phase locked loop is considered here.For the understanding of the students, the control schemes are applied in three different cases with the help of matlab simulink and the results are analyzed.The authors hope that the electrical engineering students may gain the knowledge about the effective use of solar energy and also grid synchronization technique for a reliable power supply to the critical loads in the topic control schemes on photovoltaic integrated grid system and proceed for their project or research work.The suggested scheme is duly simulated in matlab simulink environment and also realized as a hardware prototype; the simulation and hardware results reveal that the suggested approach provides clear directions for a resilient operation of a micro-grid that embodies necessary renewable sources and storage elements.

Journal: International Journal of Electrical Engineering & Education (2019)
Publication types: Article in Press;Research Article;
Wavelet transform approach for image processing – A research motivation for engineering graduates

The study of wavelet transform and its application to image processing are being incorporated in undergraduate and postgraduate syllabuses.This article discuss the basic concepts of image processing and its importance in real time application.The content of this work elaborates the importance of the image denoising and what is the necessity of image denoising in transmitted images in image enhancement topic among the final year project students as well as an emerging research topic among the research scholars worldwide.Hence this article is prepared in order to trigger those students’ knowledge in basic steps involved in image processing techniques in detail.This study highly focuses on how wavelet transform is involved in denoising and impact of denoising in an image.In general, the basic transforms like fourier transform and discrete cosine transforms are used to transform the image from one domain to another domain.Further it does not require a priori knowledge of noisy image over a transmission time.Hence the proposed method can provide a satisfactory improvement in image enhancement, assured reduction in root mean square error and improvement in the peak signal-to-noise ratio.The peak signal-to-noise ratio value of noisy image to denoised images is improved more in different wavelet transform methods.Thus, this paper can help the students who are working with visual perception of images and machine learning algorithm under image processing environment.

Journal: International Journal of Electrical Engineering & Education (2019)
Publication types: Article in Press;Research Article;
Thermal plasticity deformation buckling analysis on the carbon fiber-reinforced aluminum matrix composite material

As light weight and durability have become the important design objectives for the modern aircraft structures, the carbon fiber-reinforced resin matrix composites are widely applied to the aircraft structures for it can reduce the airframe weight effectively at the same time of guaranteeing the load-carrying capacity.In aircraft structures, the mechanical connection remains the important connection pattern for the composite material and the laminated structures; however, existence of the connecting hole has resulted in the stress concentration of structures while the borehole fatigue breakdown of parts is the main failure mode of aircraft structures.According to the low fatigue life of the aircraft connection structures, research on the hole extrusion and reinforcement technology on the carbon fiber-reinforced composite material and the laminated structure is made in this paper.

Journal: International Journal of Electrical Engineering & Education (2019)
Publication types: Article in Press;Research Article;