syedzayyan/paper_mill_benchmark huggingface dataset:
A delay-bounded low-cost multicast routing algorithm for mobile ad hoc networks

In this paper, we focus on developing delay-bounded low-cost multicast tree, linking a source to a set of multicast nodes in a mobile ad hoc network the problem of our concern is given a wireless ad hoc network and a multicast request, how to find a multicast tree such that the total cost of the multicast tree is minimized, the cost can be regarded as transmitting power, bandwidth, battery energy, and so on.We proposed a novel minimum-cost multicast routing heuristic algorithm (delay-bounded low-cost multicast routing algorithm) to solve this well-known hard problem, and this algorithm can be easily implemented in a distributed fashion.The algorithm guarantees the multicast tree, which satisfies the delay bound and has low cost if it exists.And finally, the performance of mobile ad hoc network scared from 50 to 200 nodes is evaluated.Simulations results show that the delay and tree cost of the proposed method are lower than well-known multicast ad hoc on-demand distance vector protocol and qo s dependent multicast routing protocol.

Journal: International Journal of Electrical Engineering & Education (2019)
Publication types: Article in Press;Research Article;
Research on financial risk linkage measurement based on VAR method and EVT theory

The financial market of china has gradually become matured in recent years, while the price of financial risks has also arisen accordingly, how to give play to the currency regulation and control in the financial market field has become the key topic faced by various-level governments, this paper puts forward a financial market price equilibrium model estimation method based on fb bayes algorithm.Firstly, this paper, based on the effective restriction of financial institutions, financial market institutions, and credit agencies on the financial market price, establishes a financial market price equilibrium model estimation model, puts forward a fb compressed bayes filtering algorithm to effectively handle the noise and uncertainty problems existed in the financial market price, realize the effective estimation on the financial market price equilibrium model, deeply analyzes the influence of monetary policy on the price trend of domestic financial market.The experiment result shows that in view of the financial risk prevention of the financial market price in our country, the country should formulate prudent polities for cooperation to effectively reduce the credit value ratio of financial market field.

Journal: International Journal of Electrical Engineering & Education (2019)
Publication types: Article in Press;Research Article;
Development of a pedagogical framework to analyze the performance of induction machines

This paper elucidates a procedure for ascertaining the performance of induction generators to disseminate the inevitable role of an induction machine in an alternate energy conversion among undergraduate and postgraduate students.Initially, a circle diagram is constructed for generator mode and motor mode using a drawing tool—auto cad from a simplified, energy consumption predetermination test data.Later a set of data is derived and is trained using an innovative, powerful pedagogical tool-radial basis function neural network to predict the performance indices of an induction machine.The proposed technique is compared with the experimental results of a three-phase 5 hp induction machine for various loaded conditions.Further, it provides encouraging results that will help the manufacturers to become more involved in the performance strategies of induction machines.Thus, the noninvasive method avoids the manipulation of an algebraic equation of nth order polynomial equations in predicting the generator performance indices.

Journal: International Journal of Electrical Engineering & Education (2019)
Publication types: Article in Press;Research Article;
Ergonomics of work space: Changing dynamics on OCRA evaluation and work aids

The work space is the sancto sanctorum of the world’s economy.It has become increasingly clear that a functional, efficient work space is conducive to optimum output.Therefore the engineering concepts of comfort and ease of working for long durations have become the mainstay of industries the world over.These concepts are complemented by the inputs of safety, hygiene and worker friendliness especially in the global garment manufacturing industry.They need to be evaluated scientifically.One of these means is the evaluation by using ocra.The basic concept of ocra, therefore, is to determine and measure a defined technical action.It examines the series of complex movements necessary to complete a work task, involving the upper limb joints at the shoulder, elbow, wrist and finger level.Various scholars have documented the root causes of musculoskeletal discomfort among the seated workers and have delineated them as the constrained work posture and repetitive tasks.Currently, the researcher is working on three work aid prototypes by incorporating workstation design principles for modifications to improve working posture.

Journal: International Journal of Electrical Engineering & Education (2020)
Publication types: Article in Press;Research Article;
Co-ordinated and real time voltage control of DSTS-biomass based isolated hybrid power system using Grey Wolf optimization

This paper proposes a co-ordinated reactive power control strategy analysis in line side converter (lsc) of doubly fed induction generator (dfig) and in the automatic voltage regulator (avr) of synchronous generator (sg) in isolated dish stirling solar thermal(dsts) – biomass hybrid power system.This co-ordinated control strategy greatly maintains the autonomous hybrid power system voltage deviation by adjusting the reactive power imbalance caused due to the impulsive reactive power nature of dfig coupled with the dsts and load.Gain values of integral order proportional integral (io-pi) controller in lsc and avr are optimized to maintain the system voltage stability.Modern and simple grey wolf optimization algorithm has been used to extract the superior pi gain values to maintain system stability with less reaction time.The proposed co-ordinated control strategy has been tested with various load disturbance and with dynamic input power feed from dsts to validate the system robustness.Also, the optimized gain value based voltage deviation values are validated in real time with d space.

Journal: International Journal of Electrical Engineering & Education (2020)
Publication types: Article in Press;Research Article;
Proteomic analysis on N, N'-dinitrosopiperazine-mediated metastasis of nasopharyngeal carcinoma 6-10B cells

Background nasopharyngeal carcinoma (npc) has a high metastatic feature.N,n ′ -dinitrosopiperazine (dnp) is involved in npc metastasis, but its mechanism is not clear.The aim of this study is to reveal the pathogenesis of dnp-involved metastasis.6-10b cells with low metastasis are from npc cell line sune-1, were used to investigate the mechanism of dnp-mediated npc metastasis.Results 6-10b cells were grown in dmem containing 2 h 4 -l-lysine and 13 c 6 15 n 4 -l-arginine or conventional l-lysine and l-arginine, and identified the incorporation of amino acid by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Labeled 6-10b cells were treated with dnp at 0 -18 μm to establish the non-cytotoxic concentration (ncc) range.Ncc was 0 -10 μm.Following treatment with dnp at this range, the motility and invasion of cells were detected in vitro , and dnp-mediated metastasis was confirmed in the nude mice.Dnp increased 6-10b cell metastasis in vitro and vivo .Dnp-induced protein expression was investigated using a quantitative proteomic.The silac-based approach quantified 2698 proteins, 371 of which showed significant change after dnp treatment (172 up-regulated and 199 down-regulated proteins).Dnp induced the change in abundance of mitochondrial proteins, mediated the status of oxidative stress and the imbalance of redox state, increased cytoskeletal protein, cathepsin, anterior gradient-2, and clusterin expression.Dnp also increased the expression of secretory akr1b10, cathepsin b and clusterin 6-10b cells.Gene ontology and ingenuity pathway analysis showed that dnp may regulate protein synthesis, cellular movement, lipid metabolism, molecular transport, cellular growth and proliferation signaling pathways.Conclusion dnp may regulate cytoskeletal protein, cathepsin, anterior gradient-2, and clusterin expression, increase npc cells motility and invasion, is involved npc metastasis.

Journal: BMC Molecular and Cell Biology (2012)
Publication types: Research Article;
Down-regulated lncRNA DLX6-AS1 inhibits tumorigenesis through STAT3 signaling pathway by suppressing CADM1 promoter methylation in liver cancer stem cells

Background liver cancer stem cells (lcscs) are a small subset of cells characterized by unlimited self-renewal, cell differentiation, and uncontrollable cellular growth.Lcscs are also resistant to conventional therapies and are thus believed to be held responsible for causing treatment failure of hepatocellular carcinoma (hcc).It has been recently found that long non-coding rnas (lnc rnas) are important regulators in hcc.This present study aims to explore the underlying mechanism of how lnc rna dlx6-as1 influences the development of lcscs and hcc.Methods a microarray-based analysis was performed to initially screen differentially expressed lnc rnas associated with hcc.We then analyzed the lnc rna dlx6-as1 levels as well as cadm1 promoter methylation.The m rna and protein expression of cadm1, stat3, cd133, cd13, oct-4, sox2, and nanog were then detected.We quantified our results by evaluating the spheroid formation, proliferation, and tumor formation abilities, as well as the proportion of tumor stem cells, and the recruitment of dna methyltransferase (dnmt) in lcscs when lnc rna dlx6-as1 was either overexpressed or silenced.Results lnc rna dlx6-as1 was upregulated in hcc.The silencing of lnc rna dlx6-as1 was shown to reduce and inhibit spheroid formation, colony formation, proliferation, and tumor formation abilities, as well as attenuate cd133, cd13, oct-4, sox2, and nanog expression in lcscs.Furthermore, downregulation of lnc rna dlx6-as1 contributed to a reduction in cadm1 promoter methylation via suppression of dnmt1, dnmt3a, and dnmt3b in lcscs and inactivating the stat3 signaling pathway.Conclusion this study demonstrated that down-regulated lnc rna dlx6-as1 may inhibit the stem cell properties of lcscs through upregulation of cadm1 by suppressing the methylation of the cadm1 promoter and inactivation of the stat3 signaling pathway.

Journal: Journal of Experimental & Clinical Cancer Research: (CR) (2019)
Publication types: Research Article;
Identification of novel signaling components in N,N'-Dinitrosopiperazine-mediated metastasis of nasopharyngeal Carcinoma by quantitative phosphoproteomics

Background nasopharyngeal carcinoma (npc) is a highly invasive and metastatic cancer.N,n’ - dinitrosopiperazine (dnp), a carcinogen with specificity for nasopharyngeal epithelium, facilitates npc metastasis.However, the underlying mechanism is not known.Methods quantitative phosphoproteomics, using stable isotope labeling of amino acids in cell cultures, was employed to identify phosphoproteins associated with npc metastasis mediated by dnp.Npc cell line 6-10b, which is relatively less metastatic, was used to investigate dnp-mediated metastasis.Boyden chamber invasion assay was used to measure dnp-induced motility and invasion, and nude mice were used to verify dnp-mediated metastasis in vivo.Several different phosphoproteins detected by proteomics analysis were verified by immunoblotting.Dnp-mediated metastasis facilitated by lysine-rich ceacam1 co-isolated protein (lyric) phosphorylation at serine 568 was confirmed using mutations targeting the phosphorylation site of lyric.Dnp-mediated metastasis through lyric phosphorylation was confirmed in the npc cell line cne1.Dnp-mediated lyric phosphorylation at serine 568 was also verified in metastatic tumors of babl/c nude mice.Results boyden chamber invasion assay indicated that dnp mediated cell motility and invasion of npc cell 6-10b in vitro , and experiments with nude mice indicated that dnp increased 6-10b metastasis in vivo.In the phosphoproteomics analysis, we detected 216 phosphorylation sites on 130 proteins; among these, 48 phosphorylation sites on 30 unique phosphopeptides were modulated by dnp by at least 1.5-fold.Dnp mediated the expression of phosphorylated gtpase, ferritin, lyric, and rna polymerase, and it decreased the expression of phosphorylated torsin-1a protein 1.Furthermore, dnp induced lyric phosphorylation at serine 568 to facilitate cell motility and invasion, whereas dnp-mediated motility and invasion was decreased when serine 568 in lyric was mutated.In another npc cell line, cne1, dnp also mediated cell motility and invasion followed by enhanced phosphorylation of lyric at serine 568.Finally, phosphorylated-lyric expression at serine 568 was significantly increased in metastatic tumors induced by dnp.Conclusion dnp regulates multiple signaling pathways through protein phosphorylation, including the phosphorylation of lyric at serine 568, and mediates npc metastasis.These findings provide insights on the complexity and dynamics of dnp-facilitated metastasis, and may help to gain a better understanding of the mechanisms by clarifying npc-induced metastasis.

Journal: BMC Cancer (2014)
Publication types: Research Article;
A candidate Chinese medicine preparation-Fructus Viticis Total Flavonoids inhibits stem-like characteristics of lung cancer stem-like cells

Background cancer stem cells (cscs) are considered as the origin of tumor relapse.Here, we investigated the effects of fructus viticis total flavonoids (fvtf) on the characteristics of lung cancer stem-like cells (lcslcs) derived from human small cell lung cancer nci-h446 cell line and its potential mechanism.Methods human small cell lung cancer nci-h446 cell line was cultured in vitro.The cd133 + cells were sorted from nci-h446 cell line by magnetic separation.The suspended culture with stem cell-conditioned medium was used to amplify cd133 + sphere-forming cells (sfcs).The stem cell characteristics of cd133 + sfcs were evaluated using cell self-renewal capacity by tumor sphere formation assay, migration and invasion capacity by transwell assay, tumorigenicity by xenograft model in nude mouse and cancer stem cell markers expression levels by western blot.The effects of fvtf on the properties of lcslcs were examined by tumorsphere formation assay and transwell chamber assay.The expression level of p-akt was determined by western blot analysis.Result cd133 + sfcs derived from human small cell lung cancer nci-h446 cells exhibited stemness properties of tumorsphere formation and tumorigenesis capacity comparing to the parental cells.Fvtf relative selectively inhibited the proliferation of lcslcs, suppressed tumor sphere forming capacity and migration and invasion of lcslcs, and down-regulated the protein expression of stem cell markers (cd133, cd44 and aldh1), self-renewal associated transcription factors (bmi1, nanog and oct4) and invasion associated transcription factors (twist1 and snail1) in a dose-dependent manner.Moreover, we found that fvtf treatment could significantly decrease the phosphorylation level of akt in lcslcs.Meanwhile, ly294002 and fvtf synergistically inhibited the characteristics of lcslcs.Conclusion fvtf inhibits the characteristics of lcslcs through down-regulating expression of p-akt.

Journal: BMC Complementary Medicine and Therapies (2016)
Publication types: Research Article;
Matrine derivate MASM uncovers a novel function for ribosomal protein S5 in osteoclastogenesis and postmenopausal osteoporosis

Postmenopausal osteoporosis (pomp) is a public health problem characterized by decreased bone density and increased fracture risk.Over-activated osteoclastogenesis plays a vital role in pomp.Here we developed a novel bioactive compound masm (m19) based on sophocarpine.Although it showed no significant effects on osteogenesis and adipogenesis for bone marrow-derived mesenchymal stem cells (bmscs) in vitro , it could significantly inhibit rankl/m-csf induced osteoclastogenesis through suppressing nf- κ b, mapks and pi3k/akt pathways in vitro and ameliorate bone loss in ovariectomized mice in vivo .Ribosomal protein s5 (rps5) has been identified as a target of m19 and regulates pi3k/akt, nf- κ b and mapks pathways in osteoclastogenesis.Overexpressions of rps5 synergistically inhibited osteoclastogenesis with m19 while silencing rps5 compromised m19 inhibitory effects on osteoclastogenesis in vitro .Among the three pathways, akt plays a major role in m19 effects.The akt activator sc 79 partially reversed the inhibitory effects on osteoclastogenesis by m19 and rps5-knocking-down.It indicates that rps5 serves as a potential candidate target for inhibiting osteoclastogenesis and osteoporosis therapy and m19 is a promising agent for pomp treatment.

Journal: Cell Death & Disease (2017)
Publication types: Research Article;
Intestinal fatty acid-binding protein mediates atherosclerotic progress through increasing intestinal inflammation and permeability

Atherosclerosis is one of leading phenotypes of cardiovascular diseases, featured with increased vascular intima‐media thickness (imt) and unstable plaques.The interaction between gastrointestinal system and cardiovascular homeostasis is emerging as a hot topic.Therefore, the present study aimed to explore the role of an intestinal protein, intestinal fatty acid‐binding protein (i‐fabp/fabp2) in the atherosclerotic progress.In western diet–fed apo e −/− mice, fabp2 was highly expressed in intestine.Silence of intestinal fabp2 attenuated western diet–induced atherosclerotic phenotypes, including decreasing toxic lipid accumulation, vascular fibrosis and inflammatory response.Mechanistically, intestinal fabp2 knockdown improved intestinal permeability through increasing the expression of tight junction proteins.Meanwhile, intestinal fabp2 knockdown mice exhibited down‐regulation of intestinal inflammation in western diet–fed apo e −/− mice.In clinical patients, the circulating level of fabp2 was obviously increased in patients with cardiovascular disease and positively correlated with the value of carotid intima‐media thickness, total cholesterol and triglyceride.In conclusion, fabp2‐induced intestinal permeability could address a potential role of gastrointestinal system in the development of atherosclerosis, and targeting on intestinal fabp2 might provide a therapeutic approach to protect against atherosclerosis.

Journal: Journal of Cellular and Molecular Medicine (JCMM) (2020)
Publication types: Research Article;
MiRNA-107 enhances the malignant progression of pancreatic cancer by targeting TGFBR3

The prognosis of pancreatic cancer (pc) is relatively dismal due to the lack of effective therapy.In this study, we explored the specific functions and molecular mechanisms of mi r-107 to uncover effective therapeutic targets for pc.Method the mi r-107 expression in pc cell lines was assessed via quantitative real-time polymerase chain reaction (q rt-pcr).Besides, online bioinformatics analysis was adopted to predict the underlying targets of mi r-107.Meanwhile, tcga database was employed to explore the prognosis of pc patients.In addition, mtt and transwell assays were conducted to explore the pc cells’ biological functions.Result mi r-107 was remarkably increased in pc cells which could promote the proliferation, invasion and migration of pc cells.In addition, mi r-107 could directly down-regulate tgfbr3 expression through binding to tgfbr3 3’utr.Survival analysis from tcga suggested that pc patients with higher mi r-107 expression was significantly involved in poorer prognosis.Conclusion we concluded that mi r-107 promoted proliferation, invasion and migration of pc cells via targeting tgfbr3, which may provide novel underlying therapeutic targets.

Journal: PLoS One (2021)
Publication types: Research Article;
MicroRNA-431 inhibits migration and invasion of hepatocellular carcinoma cells by targeting the ZEB1-mediated epithelial–mensenchymal transition

Micro rna‐431 (mi r‐431) has been recognized as an oncogenic mi rna, being implicated in the initiation and development of human cancers.Recently, deregulation of mi r‐431 has been reported in several tumors.However, the clinical significance of mi r‐431 and its underlying role in human hepatocellular carcinoma (hcc) are poorly explored.Herein, we found that mi r‐431 expression was reduced in hcc tissues compared to noncancerous tissues.Otherwise, down‐regulation of mi r‐431 was observed in aggressive tumor tissues.The levels of mi r‐431 expression in hcc cell lines were significantly lower than that in a nontransformed hepatic cell line.Clinical association analyses disclosed that a low level of mi r‐431 was prominently associated with poor prognostic features of hcc including venous infiltration, high edmondson–steiner grading and advanced tumor‐node‐metastasis (tnm) tumor stage.Our in vitro studies showed that up‐regulation of mi r‐431 expression reduced cell invasion and migration in hcclm3 cells.In contrast, down‐regulation of mi r‐431 expression promoted smmc‐7721 cell invasion and migration.We found that up‐regulation of mi r‐431 expression decreased zinc finger e‐box binding homeobox 1 (zeb1) expression and inhibited the epithelial–mesenchymal transition (emt) with increased e‐cadherin expression and decreased vimentin expression in hcclm3 cells.Otherwise, down‐regulation of mi r‐431 expression increased zeb1 expression and promoted emt in smmc‐7721 cells.Significantly, zeb1 was identified as a target of mi r‐431 in hcc.Zeb1 knockdown abrogated the effect of mi r‐431 silencing on emt and cell mobility in smmc‐7721 cells.In conclusion, mi r‐431 inhibits migration and invasion of hcc cells by suppressing zeb1‐mediated emt.

Journal: FEBS Open Bio (2015)
Publication types: Research Article;
MicroRNA-520g induces epithelial-mesenchymal transition and promotes metastasis of hepatocellular carcinoma by targeting SMAD7

Hepatocellular carcinoma (hcc) is the third leading cause of cancer‐related death worldwide.Aberrant expression of mi rnas contributes to hcc development.Here, we observed elevated mi r‐520g expression in tumor samples from hcc patients with relapse and metastasis, and this high mi r‐520g expression was correlated with poor survival.Through gain‐ and loss‐of‐function studies, mi r‐520g was demonstrated to facilitate hcc cell migration, invasion and epithelial–mesenchymal transition (emt).Smad7 was identified as a direct target of mi r‐520g.Accordingly, we conclude that high mi r‐520g expression promotes hcc cell mobility and emt by targeting smad7, and this is correlated with reduced survival in hcc patients.

Journal: FEBS Letters (2014)
Publication types: Research Article;
MiR-365b-3p, down-regulated in retinoblastoma, regulates cell cycle progression and apoptosis of human retinoblastoma cells by targeting PAX6

Pax6 contributes to the development and progression of retinoblastoma (rb), but the molecular mechanism underlying the regulation of pax6 expression is unclear.Here we found that micro rna‐365b‐3p (mi r‐365b‐3p) is downregulated in human rb tissues.Ectopic expression of mi r‐365b‐3p significantly attenuates cell growth, induces cell cycle arrest in g1 phase and cell apoptosis through inhibiting the expression of pax6 by directly binding its 3′ untranslated regions.Furthermore, overexpression of mi r‐365b‐3p upregulates p21 and p27 but downregulates cdc2 and cyclin d1 protein levels.Elucidating the regulatory mechanism of pax6 by micro rnas may give new clues to the therapy against rb.

Journal: FEBS Letters (2013)
Publication types: Research Article;