syedzayyan/paper_mill_benchmark huggingface dataset:
KCNQ1OT1 Influences HK-2 Apoptosis and Inflammation in LPS-Induced Acute Renal Injury via Modulating miR-30a-5p/NLRP3 Axis

To investigate the influence of kcnq1ot1 on hk-2 apoptosis and inflammation in ari and its molecular mechanism.Methods.Normal cultivated hk-2 cells were used as negative control (nc) group.Three different concentrations of lipopolysaccharide (lps) were used to treat the cells (5 μg/m l, 10 μg/m l, and 20 μg/m l).The groups included si-kcn1ot1+ lps, si-nc + lps, mi r-30a-5p + lps, pc dna-nlrp3+si-kcnq1ot1 + lps group, mi r-nc + lps group, and pc dna + si-kcnq1ot1 + lps group.Cck-8 and flow cytometry are used to measure cell viability and apoptosis, while rt-q pcr and western blotting are used to detect kcnq1ot1, mi r-30a-5p, and nlrp3 m rna.Elisa was used to detect the levels of tnf-α, il-6, and il-1β in hk-2 cells.The targeting relationship among kcnq1ot1, mi r-30a-5p, and nlrp3 was verified.Results.After the intervention of lps, the viability of hk-2 cells was decreased, while the apoptosis rates were increased.The m rna and protein expressions of nlrp3 and kcnq1ot1 were increased, while the m rna and protein levels of mi r-30a-5p were decreased ( p &lt; 0.05 ).The expressions of bax and cleaved-caspase-3 were downregulated after silencing kcnq1ot1 and overexpressed mi r-30a-5p.In addition, the viability of hk-2 cells was improved, and the apoptosis was reduced by inhibiting kcnq1ot1 and overexpressed mi r-30a-5p.Thus, kcnq1ot1 modulated nlrp3 via targeting mi r-30a-5p.Overexpression of nlrp3 reverses kcnq1ot1 inhibition of lps-induced apoptosis, activity, and inflammation in hk-2 cells.Conclusions.Through modulating the mi r-30a-5p/nlrp3 axis, inhibition of kcnq1ot1 may reduce hk-2 apoptosis and inflammation in lps-induced ari.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Research Article;
Investigation of the Effect of Oral Implant Surgery on Clinical Treatment and Oral Function of Patients with Dentition Loss

In order to investigate the effect of oral implant surgery on clinical treatment and oral function of patients with dentition loss, a total of 118 patients with dentition loss in the department of stomatology of our hospital from january 2019 to january 2022 are retrospectively analyzed.They are randomly divided into the conventional group and the implant group.The conventional group is treated with conventional dentition restoration, and the implant group is treated with oral implant restoration.The repair efficiency of the two groups is compared.The swallowing function of the 2 groups is assessed by standard swallowing assessment (ssa) table before and after treatment.Self‐rating depression scale (sds) and self‐rating anxiety scale (sas) are used to compare the negative emotions of the two groups before and after treatment.Experimental results show that the total effective rate of the implant group is significantly higher than that of the conventional group, but no invalid cases occurred in the two groups after treatment.

Journal: Contrast Media & Molecular Imaging (2022)
Publication types: Research Article;
Integrated Wiring System of Intelligent Building Based on Internet of Things

In order to explore how the intelligent building can realize the integrated wiring system, the author proposes an integrated wiring system for the intelligent building based on the internet of things.This method recommends key technical problems and solutions based on the information represented by the internet of things and explores the research on the realization of integrated wiring systems in intelligent buildings.Research has shown that the integrated wiring system of intelligent buildings based on the internet of things can solve the shortcomings of traditional buildings, and the happiness index of occupants has increased by about 15%.In the future, a building will be a platform, and each building will be an io t unit and a cell body, making the internet of buildings possible and building a big data platform for the internet of buildings.

Journal: Wireless Communications and Mobile Computing (2022)
Publication types: Research Article;
G Protein Subunit Gamma 5 Is a Prognostic Biomarker and Correlated with Immune Infiltrates in Hepatocellular Carcinoma

Hepatocellular carcinoma (hcc) is one of the malignancies with an extremely inferior prognosis in the abdominal cavity, making it essential to develop more effective biomarkers for hcc.Although gng5 has been linked to increased patient survival in a variety of human malignancies, no evidence has been found for its involvement in the development of hcc yet.Our study first analyzed the expression and prognosis of gng5 in hcc using the cancer genome atlas database (tcga database) with the gene expression omnibus database (geo database) and found that gng5 has a potential oncogenic role.Based on survival analysis, the clinical importance and prognostic value of the gng5 gene were studied.Relying on tumor immune estimation resource database (timer database), we analyzed the correlation between the gng5 gene and hcc immune infiltration cells.Gng5 expression levels were significantly higher in hcc tissues compared to normal liver tissues.Hcc patients with high gng5 expression had significantly reduced overall survival time and affected multiple immune cell infiltrates.Additionally, kegg functional enrichment analysis indicated the pi3k-akt signaling pathway as the most promising carcinogenic pathway associated with gng5.This is the first comprehensive revelation of gng5 as a possible new biological marker associated with immune infiltration in hcc.Additionally, it holds promise as an emerging target for hcc immunotherapy.

Journal: Disease Markers (2022)
Publication types: Research Article;
Flexible Ureteroscopy and Nephroscopy for Stone Removal in Patients with Multiple Renal Calculi

Renal calculi are a common type of urological calculi and are associated with morbidity.This study was aimed at exploring the effect of flexible ureteroscopy and nephroscopy on stone removal in patients with multiple renal calculi.Method.This randomized controlled trial included a total of 78 cases with multiple renal calculi in our hospital.The patients were randomly divided into the study and control groups and treated with flexible ureteroscopy and percutaneous nephrolithotomy with pneumatic ballistics, respectively.The surgery condition, levels of prostaglandin f2α (pgf2α), prostaglandin e2 (pge2), keratocyte growth factor (kgf), renal function indices, and the incidence of complications were analyzed before and after surgery in the two groups.Result.The operation time, the postoperative analgesia pump application time, one-time stone removal rate, the intraoperative blood loss, and hospital stay of the study group were significantly lower than those of the control group.Postsurgery, the levels of pge2, pgf2α, and kgf in the study group were significantly lower than those in the control group.The serum levels of scr, bun, and ngal in the study group were significantly lower than those in the control group.In addition, the incidence complications in the study group were significantly lower.Conclusion.Flexible ureteroscopy and laser lithotripsy under nephroscopy were equally effective against multiple renal calculi.Flexible ureteroscopy reduced surgical trauma without affecting renal function and had a low incidence of complications.

Journal: Disease Markers (2022)
Publication types: Research Article;
Eye Sign is an Alternative for Evaluation of the Microcirculation in Patients with Systemic Sclerosis

S.To investigate consistency between eye sign and nailfold video capillaroscopy (nvc) in systemic sclerosis (ssc) patients and to evaluate eye sign with various parameters of disease and potential pulmonary involvement.Methods.A total of 60 ssc patients and 20 healthy individuals were enrolled.20 ssc patients were complicated with pulmonary arterial hypertension (pah); 20 ssc patients with interstitial lung diseases (ild); 20 ssc patients without pulmonary involvement.All subjects were assessed using the methods of nvc and eye sign.Skin involvement was evaluated by modified rodnan skin score (m rss) and disease activity according to medsger’s severity score (mss).Clinical manifestations and the presence of autoantibodies were carefully recorded.Any correlations between were evaluated with the spearman correlation coefficient test.Results.According to the morphological changes revealed by nvc, 3 types of nvc patterns have been characterized (early pattern, active pattern, and late pattern).Eye sign showed distinguishing morphologic changes in three patterns of nvc and pulmonary involvements (pah vs ild) in ssc.A positive linear correlation was found between scores of eye sign and nvc in all patients with ssc (r = 0.629, p = 0.001 ).A positive correlation of eye sign was found in all ssc patients with m rss (r = 0.748, p = 0.045 ) and mss (r = 0.636, p = 0.001 ).Conclusions.The study demonstrates that eye sign had a high consistency with nvc for the evaluation of the microcirculation in ssc patients and exhibited specific patterns in the early, active, and late phases of ssc.Eye sign can be used as a reliable method to classify and monitor ssc patients and replace the measurement of nvc.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Clinical Study;
Establishment of Difficult Caudal Epidural Blockade Prediction Model

We aimed to develop a predictive difficult caudal epidural blockade (p dceb) model when ultrasound was not available and verified the role of ultrasound in difficult caudal epidural blockade (ceb).Methods.From october 2018 to march 2019, this study consisted of three phases.First, we prospectively enrolled 202 patients scheduled to undergo caudal epidural anesthesia and assessed risk factors by binary logistic regression to develop the predictive scoring system.Second, we enrolled 87 patients to validate it.The receiver operating characteristic (roc) curve was used to evaluate the performance of the prediction model.Youden-index was used to determine the cut-off value.Third, we enrolled 68 patients with a high risk of difficult ceb (p dceb score ≥3) and randomized them into ultrasound and landmark groups to verify the role of ultrasound.Result.The rate of difficult ceb was 14.98% overall 289 patients.We found a correlation between unclear palpation of the sacral hiatus (or 9.688) and cornua (or 4.725), the number of the sacral hiatus by palpation ≥1 (or 4.451), and history of difficult ceb (or 39.282) with a higher possibility of difficult ceb.The area under the receiver operating characteristic curve of the p dceb model involving the aforementioned factors was 0.889 (95% ci, 0.827–0.952) in the development cohort and 0.862 (95% ci, 0.747–0.977) in the validation cohort.For patients with a p dceb score ≥3, a preprocedure ultrasound scan could reduce the incidence of difficult ceb (55.56% in the landmark group vs.9.38% in the ultrasound group, p &lt; 0.001 ).Conclusion.This novel p dceb score, which takes into account palpation of the sacral hiatus/cornua, number of the sacral hiatus by palpation ≥1, and history of difficult ceb, showed a good predictive ability of difficult ceb.The findings suggested that performing an ultrasound scan is essential for patients with a p dceb score ≥3.Trial registration: no: chi ctr1800018871, site url: principal investigator: jialian zhao, date of registration: 2018.10.14.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Clinical Study;
Efficacy of Self-Made Hewei Decoction for Chronic Atrophic Gastritis and Its Effect on Gastrin and Pepsinogen Expression Levels

We aim to investigate the therapeutic effect of self‐made hewei decoction based on the differences‐in‐differences (did) model for treating chronic atrophic gastritis and its effect on the gastrin and pepsinogen expression.A total of 166 chronic atrophic gastritis patients treated in our hospital from january 2019 to september 2020 were recruited and randomly assigned to a treatment group and a control group ( n = 83 per group).In the control group, patients were given conventional western medicine.In the treatment group, patients were administered self‐made hewei decoction besides conventional western medicine.The general data, total effective rate, and histological changes before and after treatment were compared between the two groups.The did model was utilized to compare the changes in three items of gastric function and traditional chinese medicine (tcm) syndrome scores at various time points (before treatment and 1, 3, 6, and 12 months after treatment) between the two groups.In the treatment group, 5 cases were lost to follow‐up, and 78 cases remained.In the control group, 4 cases were lost to follow‐up, and 79 cases remained.The total effective rate in the treatment group (93.59%) was significantly higher than that in the control group (82.28%) ( p &lt; 0.05).The histological score was not significantly different between the two groups before treatment ( p &gt; 0.05).The histological score at 12 months after treatment was lower than those before treatment in the two groups ( p &lt; 0.05).The histological score was lower in the treatment group than that in the control group ( p &lt; 0.05).After treatment, the tcm syndrome score and pgr17 level were lower in the treatment group than those in the control group at each time point ( p &lt; 0.05).Pgi and pgii levels were higher in the treatment group than those in the control group at each time point ( p &lt; 0.05).Did regression model showed that tcm syndrome score and pgr17 level decreased by 106.2% and 65.8%, respectively, after treatment in patients with chronic atrophic gastritis ( p &lt; 0.05), but the treatment mode was opposite to the overall therapeutic effect in terms of time.The pgi and pgii levels increased by 102.9% and 97.8%, respectively, in patients with chronic atrophic gastritis ( p &lt; 0.05), and the treatment mode was the same as the overall therapeutic effect in terms of time.There was no significant difference in the therapeutic effect between the two groups at 1 month after treatment.A significant difference in the therapeutic effect was detected between the two groups at 3 months after treatment, and the difference showed a decreasing trend after 6 months after treatment.Self‐made hewei decoction can improve the histological alterations in chronic atrophic gastritis, relieve clinical symptoms, and ameliorate the expression levels of three items of gastric function.

Journal: Contrast Media & Molecular Imaging (2022)
Publication types: Research Article;
Effect of Salvia Miltiorrhiza Polyphenolic Acid Injection on Improving Limb Use and Cognitive Impairment in Patients with Acute Stroke

S.To investigate the effect of injectable salvia polyphenolic acid on the improvement of limb movement and cognitive dysfunction in acute stroke patients.Materials and methods.The clinical data of 90 acute stroke patients were collected for retrospective study and divided into 45 cases each in the comparison group and the observation group according to the different treatment methods; using basic treatment + salvianolic acid, the comparison group implemented conventional alteplase and butalbital treatment, and the observation group used injectable salvianolic acid treatment, to observe and compare the clinical efficacy, changes in neurological deficits, cognitive function, and motor function scores before and after treatment in the two groups.Results.The nihss (national institute of health stroke scale) score, cerebral infarct volume, nse (neuron-specific enolase), and s100β (a neurotrophic factor) levels were reduced after treatment compared with those before treatment in this group, and the nihss score, cerebral infarct volume, nse, and s100β levels in the observation group were lower than those in the comparison group after treatment, and the difference was statistically significant ( p &lt; 0.05 ).Compared with the clinical efficacy of the comparison group and the observation group, the treatment effect of the observation group was better than that of the comparison group, and the difference was statistically significant ( p &lt; 0.05 ).After treatment, the cognitive function and motor function scores of both groups were significantly improved compared with those before treatment, and the degree of improvement of each score in the observation group was significantly better than that in the comparison group ( p &lt; 0.05 ).During the trial, two patients in the comparison group developed a generalized rash and withdrew from the experiment, and the rash subsided after anti-allergic treatment, and no significant adverse events were observed in the remaining participants.There was no statistically significant difference in liver and kidney function and cardiac enzyme test indexes between the two groups of patients at 14 days of treatment ( p &gt; 0.05 ).Conclusion.Danshen polyphenolic acid for injection has definite clinical efficacy in the treatment of acute ischemic stroke, and it can effectively improve cognitive and motor functions and promote neurological recovery in patients with high safety.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
Effect of HIV-1 Protease Inhibitor on IL-18 and IL-1β in Rats with Insulinoma

This study is aimed at investigating the treatment effectiveness of hiv-1 protease inhibitor for rats with insulinoma and its effects on interleukin-1β (il-1β) and interleukin-18 (il-18).A total of 40 6-week-old nude mice were included in this study.We randomly assigned 20 rats for insulinoma modeling and divided them into model a and b groups.Another 20 rats were randomly divided into control a and b groups.Rats from the model a and control a groups were given hiv-1 protease inhibitors.The expression profiles of il-18 and il-1β, clinical indicators, water maze test results, oxidative stress damage, and changes in neurological functions in rats from each group were recorded.The expression levels of il-18 and il-1β, insulin level, the ratio of immunoreactive insulin to plasma glucose (iri/g), escape latency, reactive oxygen species (ros), and amyloid β-protein (aβ) level were lower in the model a group than in the model b group while fasting blood glucose, platform crossing times, and superoxide dismutase (sod) were higher in the model a group than in the model b group.The insulin level and hippocampus aβ level were lower in the control a group than in the control b group.In contrast, other indicators in the control a group were not significantly different from those in the model b group.Hiv-1 protease inhibitor is effective in the treatment of insulinoma in rats.It can significantly reduce il-18 and il-1β and protect the neurological functions in rats and has broad prospects for clinical application.

Journal: Disease Markers (2022)
Publication types: Research Article;
Effect of Combined Spinal-Epidural Anesthesia and Total Intravenous Anesthesia on Hemodynamics and Pregnancy Outcomes of Severe Preeclampsia Pregnant Patients Undergoing Cesarean Section

The purpose of the study was to investigate the effect of combined spinal-epidural anesthesia (csea) and total intravenous anesthesia (tiva) on hemodynamics and pregnancy outcomes of severe preeclampsia pregnant patients undergoing cesarean section.Methods.126 patients with severe preeclampsia admitted to zhangqiu district people’s hospital from august 2018 to august 2019 were selected as the study subjects and randomly divided into the experimental group (n = 63) and control group (n = 63).After undergoing cesarean section, the patients in the experimental group received csea, while those in the control group were given tiva.After that, the effect of different anesthesia methods on the hemodynamics and pregnancy outcomes of pregnant women was compared.Results.There were no significant differences in age, bmi value, weight, height, gestational weeks, sbp, dbp, and residence between the two groups (p &gt; 0.05).The operation duration, the onset time of anesthesia, and delivery time in the experimental group were significantly shorter than those in the control group, with less intraoperative blood loss in the experimental group than that in the control group (p &lt; 0.001).In both groups, map and sp o2 during delivery were significantly lower than those before anesthesia, and hr was significantly higher than that before anesthesia (p &lt; 0.001).In the experimental group, map and hr during delivery were significantly lower than those in the control group, and sp o2 was significantly higher than that in the control group (p &lt; 0.001).The total effective rate of anesthesia in the experimental group was significantly higher than that in the control group (p &lt; 0.05).The apgar scoring of the newborns in the experimental group was significantly higher than that in the control group (p &lt; 0.001), and the total incidence of postoperative adverse reactions in the experimental group was significantly lower than that in the control group (p &lt; 0.05).Conclusion.Csea is a reliable anesthesia method for improving the hemodynamics indicators in pregnant patients with severe preeclampsia; such strategy greatly increases the apgar score of newborns and shortens the anesthesia onset time.Further research will be conducive to establishing a better anesthesia plan for such patients.

Journal: Evidence-Based Complementary and Alternative Medicine (2022)
Publication types: Clinical Study;