syedzayyan/paper_mill_benchmark huggingface dataset:
Novel Insight Into Nutritional Regulation in Enhancement of Immune Status and Mediation of Inflammation Dynamics Integrated StudyIn VivoandIn Vitroof Teleost Grass Carp (Ctenopharyngodon idella): Administration of Threonine.

This study aims to investigate the effects of threonine (thr) on immunoregulationin vivoandin vitroof teleost grass carp (ctenopharyngodon idella).Juveniles (9.53 ± 0.02 g) were reared for 8 weeks with respective thr diet (3.99, 7.70, 10.72, 14.10, 17.96, and 21.66 g/kg) and then challenged with aeromonas hydrophilaforin vivostudy.Macrophages isolated from head kidney were treatedin vitrofor 48 h with l-thr (0, 0.5, 1.0, 2.0, 4.0, and 8.0 m m) after 6 h of lipopolysaccharide induction.The results showed that, compared with thr deficiency (3.99 g/kg), the optimal dietary thr (14.10g/kg) affected the immunocyte activation in the head kidney (hk) and spleen (sp) by downregulating the m rna expressions of mhc-ii and upregulating cd4 (not cd8), and it mediated the innate immune by enhancing the activities of lysozyme (lz), acid phosphatase content of complement 3 (c3) and c4, increasing the m rna abundances of hepcidin, liver expressed antimicrobial peptide-2a (leap-2a), leap-2b, β-defensin1, downregulating tumor necrosis factor α (tnf-α), il-6, il-1β, il-12p35, il-12p40, il-17af1, and il-17d partly by attenuating rorγ1 transcriptional factor and nuclear factor kappa b p65 (nf-κbp65) signaling cascades [ikkβ/iκbα/nf-κbp65] and upregulating transforming growth factor β1 (tgf-β1), il-4/13a, -4/13b, il-10, and il-22 partly by gata-3.Besides these, the optimal dietary thr regulated the adaptive immune by upregulating the m rnas of immunoglobulin m (ig m) and ig z (not ig d).Moreover, 2 m m thr downregulatedin vitrothe m rna abundances of colony stimulating factor-1, inducible nitric oxide synthase, mannose receptor 1, matrix metalloproteinase2 (mmp-2), and mmp-9 significantly (p< 0.05), indicating that thr could attenuate the m1-type macrophages' activation.Moreover, l-thr downregulated the m rna transcripts of tnf-α, il-6, and il-1β associated with impairing the socs1/stat1 signaling and upregulated il-10 and tgf-β1 partly by accentuating the socs3/stat3 pathway.The above-mentioned observations suggested that thr improved the immune status in the immune organs of fish by enhancing the immune defense and mediating the inflammation process.Finally, based on the immune indices of lz activity in hk and c3 content in sp, the optimal thr for immune enhancement in juvenile grass carp (9.53-53.43 g) was determined to be 15.70 g/kg diet (4.85 g/100 g protein) and 14.49 g/kg diet (4.47 g/100 g protein), respectively.

Journal: Frontiers in immunology (2022)
Publication types: Journal Article
Synthesis of low-density polyethylene derived carbon nanotubes for activation of persulfate and degradation of water organic micropollutants in continuous mode.

Plastic derived carbon nanotubes (cnts) were tested as catalysts in persulfate activation for the first time.Four catalysts were prepared by wetness impregnation and co-precipitation (using al2o3, ni, fe and/or al) and implemented to grow cnts by chemical vapour deposition (cvd) using low-density polyethylene (ldpe) as carbon feedstock.A catalyst screening was performed in batch mode and the best performing cnts (cnt@ni+fe/al2o3-cp) led to a high venlafaxine mass removal rate (3.17 mg g-1h-1) in ultrapure water after 90 min (even with a mixture of micropollutants).Its degradation increased when the matrix was replaced by drinking water and negligibly affected in surface water.A composite polymeric membrane was then fabricated with cnt@ni+fe/al2o3-cp and polyvinylidene fluoride (pvdf), a high venlafaxine mass removal rate in surface water being also observed in 24 h of continuous operation.Therefore, the results herein reported open a window of opportunity for the valorisation of plastic wastes in this catalytic application performed in continuous mode.

Journal: Journal of environmental management (2022)
Publication types: Journal Article
Primary hyperparathyroidism misdiagnosed as metastatic disease, with trans-oral endoscopic surgical management.

Primary hyperparathyroidism is not so uncommon a disease.In high-income countries, with the introduction of routine biochemical testing in 1980s, it is diagnosed early, and the majority are seen as asymptomatic disease.However, in india, a severe symptomatic form is still seen, and many times patients are undiagnosed, thus continuing to suffer debilitating disease.Here, we report such a patient who was being treated as having bony metastases from an unknown primary source, when in fact he had osteitis fibrosa cystica due to primary hyperparathyroidism and was cured after surgery.

Journal: Tropical doctor (2022)
Publication types: Journal Article
3D biomaterial P scaffolds carrying umbilical cord mesenchymal stem cells improve biointegration of keratoprosthesis.

Biointegration of a keratoprosthesis (kpro) is critical for the device stability and long-term retention.Biointegration of the kpro device and host tissue takes place between the surrounding corneal graft and the central optic (made by poly (methyl methacrylate)).Our previous clinical results showed that auricular cartilage reinforcement is able to enhance the kpro biointegration.However, the auricular cartilage is non-renewable and difficult to acquire.In this study, we developed a novel type of biomaterial using a three-dimensional porous polyethylene glycol acrylate scaffold (3d biological p-scaffold) carrier with chondrocytes differentiated from induced human umbilical cord mesenchymal stem cells (h uc-mscs) and tested in rabbit corneas.The results showed h uc-mscs bear stem cell properties and coule be induced into chondrocytes, p-scaffold is beneficial to the growth and differentiation of h uc-mscs bothin vivoandin vitro.Besides, after implanting the p-scaffold into the corneal stroma, no serious immune rejection response, such as corneal ulcer or perforation were seen, suggested a good biocompatibility of p-scaffold with the corneal tissue.Moreover, after implanting p-scaffold in together with the differentiated chondrocytes into the rabbit corneal stroma, they significantly increased corneal thickness and strengthened the host cornea, and chondrocytes could stably persist inside the cornea.In summary, the 3d biological p-scaffold carrying differentiated h uc-mscs could be the preferable material for kpro reinforcement.

Journal: Biomedical materials (Bristol, England) (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
A retrospective study of the mid-term efficacy of full-endoscopic annulus fibrosus suture following lumbar discectomy.

S full-endoscopic discectomy is associated with a high risk of disc reherniation due to the poor mechanical strength of the annulus fibrosus after scar healing.It is technically difficult to place a full-endoscopic annulus fibrosus suture.We designed an annulus fibrosus suture device that can be used to suture annulus defects under microendoscopy.The present study investigated the safety and feasibility of this technology.Patients and methods we retrospectively analyzed the outcomes of patients who underwent surgical treatment for lumbar disc herniation (ldh) from january 2018 to october 2020.We compared 40 patients with ldh treated with full-endoscopic annulus fibrosus suture following lumbar discectomy (ld + afs group) with 42 patients treated with lumbar discectomy alone (ld group) regarding demographic data, symptoms, and recurrence and reoperation rates.Lumbar mri and ct were performed 3 and 12 months.A 10-point visual analog scale (vas) and the oswestry disability index (odi) was used to evaluate pain and the lumbar spine function.Results the cohort comprised 82 patients, including 40 patients in the ld + afs group and 42 in the ld group.All operations were successfully completed without serious complications.Reherniation occurred in no patients in the ld + afs group and three patients in the ld group.The vas scores for lumbar and leg pain and odi score were significantly improved postoperatively (p< 0.05).Conclusion compared with conventional lumbar discectomy, full-endoscopic annulus fibrosus suture following full-endoscopic lumbar discectomy is a safe and effective minimally invasive technique that reduces the ldh recurrence rate.

Journal: Frontiers in surgery (2022)
Publication types: Journal Article
Effects of polylactic acid (PLA) and polybutylene adipate-co-terephthalate (PBAT) biodegradable microplastics on the abundance and diversity of denitrifying and anammox bacteria in freshwater sediment.

Microplastics (mps) have been widely distributed on earth and have drawn global concern for freshwater and marine ecosystems.Biodegradable plastics have risen in popularity to replace nonbiodegradable plastics all over the world.The effects of biodegradable plastics on denitrifying and anammox bacteria in freshwater sediment remain largely unknown.In this study, water column reactors containing polylactic acid (pla) or polybutylene adipate-co-terephthalate (pbat) mps in sediment were established to simulate lake ecosystems and analyze the effects of biodegradable mps on sedimentary nitrogen transformation microorganisms.The total organic carbon (toc) concentrations in the pla and pbat groups were slightly higher than those in the control group, which might be related to the slow degradation of these two plastics.Denitrifying and anammox bacterial diversities decreased after adding mps to sediments for 30 days, and the dominant otus of these two bacteria were differentiated from the control.The abundance levels of nir s denitrifying and anammox bacteria on the pla mp surface were significantly higher than those in the other groups (p < 0.05), but they were lower in the pbat groups than in the other groups.As an excellent electron donor for the denitrification process, lactic acid release from pla degradation resulted in the enrichment of denitrifying and anammox bacteria on the mp surfaces.However, pbat led to various responses of bacteria in an anaerobic environment.In addition, the redundancy analysis results indicated that total phosphorus, toc and nitrate were strongly negatively correlated with the abundance levels of denitrifying and anammox bacteria.Our findings provided insight into the effects of mps, especially the biodegradable ones, on sedimentary nitrogen-transformation bacteria.

Journal: Environmental pollution (Barking, Essex : 1987) (2022)
Publication types: Journal Article
Plastic fantastic innate lymphoid cells.

In this issue of jem, nagasawa et al.( have undertaken a detailed study of the cell surface phenotype, transcriptional profile, and cytokine secretion of ilc progenitor populations in human peripheral blood and tissues and describe markers that highlight the heterogeneity of these chameleons.

Journal: The Journal of experimental medicine (2019)
Publication types: Journal Article;Comment
"Your Diet Defines Who You Are, Especially as a Man": Masculinity in Online Media Focused on Healthy Eating for Men.

In contexts marked by neoliberal ideology and a claimed "crisis" in men's health, men are responsibilized to be/come healthy.Eating has long been a gendered practice in western cultures, and recent cultural shifts have produced ways of eating that are both masculinized and (claimed) healthy.Online healthy eating advice, which encourages and supports men to eat healthily, is an important information source.However, such information draws on, reproduces, and/or disrupts existing meanings about men and eating.To understand contemporary representations of men and healthy eating, we examined 30 online media articles oriented specifically to this topic.Using reflexive thematic analysis from a social constructionist position, we developed two themes:a lad's looks and lifestyleand mind over matter: the masculine mindset.these themes together told an overarching story that healthy eating is effectively sold to men by drawing on traditional or hegemonic ideals of masculinity and effectively evoking access to an enhanced masculinity through healthy eating.While these representational practices may sell healthy eating to men, with likely positive health benefits, they also reinforce hegemonic ideals of masculinity which can be problematic from a health perspective.

Journal: American journal of men's health (2023)
Publication types: Journal Article
A new species of frog-biting midge from Papua New Guinea with a key to the described Corethrellidae of the Australopapuan region (Diptera, Corethrellidae,Corethrella).

Corethrellaoppositophila kvifte & bernal,sp.N.is described based on one male and six female specimens collected at 2200 m a.s.l.On mount wilhelm, papua new guinea.The species is the fourth species of frog-biting midge described from this country and appears similar to corethrellasolomonis belkin based on pigmentation of legs and abdominal tergites.It differs from c.solomonis, however, in the shape of female flagellomeres i-iii, and in the thorax which has a dark brown vertical stripe.The new species is named for its sexually dimorphic flagellomeres, which are short and squat in the female and elongate in the male.These differences in morphological characters are discussed in light of the likely sexual differences in functional uses of the antennae, as males use them for mating only whereas females use them both for mating and prey location.An emended key is presented to the described australopapuan species of corethrellidae.

Journal: ZooKeys (2018)
Publication types: Journal Article
Lung Cancer Screening Criteria and Cardiopulmonary Comorbidities.

Introduction lung cancer screening criteria should select candidates with minimal cardiopulmonary comorbidities who are fit for curative lung cancer resection.Methods we retrospectively analyzed 728 patients with lung cancer for screening eligibility using the u.s.Preventive services task force (uspstf) 2013 criteria (n = 370).If ineligible for screening, they were further assessed for eligibility using the uspstf 2021 (n = 121) and national comprehensive cancer network group 2 (nccn gp 2) (n = 155).Comparisons of cardiopulmonary comorbidities between patients selected by the different lung cancer screening criteria were performed.Excluding missing data, a similar comparison was done between uspstf 2013 (n = 283) and plcom2012 (risk threshold ≥1.51%) (n = 118).Results patients eligible for uspstf 2021 and nccn gp 2 had lower rates of airflow obstruction (forced expiratory volume in 1 s [fev1]/forced vital capacity <0.7) compared with those in uspstf 2013 (55.4% and 56.8% versus 70.5%).Both uspstf 2021 and nccn gp 2 groups had less severe airflow obstruction; only 11.6% and 12.9% of patients, respectively, had percent-predicted fev1 less than 50% versus 20.3% in the uspstf 2013 group.Comparing uspstf 2013 and plcom2012 revealed no significant differences in age or the rate of airflow obstruction (p= 0.06 andp= 0.09 respectively).Nevertheless, rates of percent-predicted fev1 less than 50% and diffusing capacity of the lungs for carbon monoxide less than 50% were lower in the plcom2012 group compared with those in the uspstf 2013 group (22.3% versus 10.2% and 32.6% versus 20.0%), respectively.Conclusions the uspstf 2021 qualifies an additional group of screening candidates who are healthier with better lung reserve, translating to better surgical candidacy but potentially more overdiagnosis.The plcom2012, with its better accuracy in selecting patients at risk of cancer, selects an older group with chronic obstructive pulmonary disease but with good lung reserve and potentially less overdiagnosis.

Journal: JTO clinical and research reports (2022)
Publication types: Journal Article
Human Platelet Lysate as Alternative of Fetal Bovine Serum for Enhanced HumanIn VitroBone Resorption and Remodeling.

Introduction to study human physiological and pathological bone remodeling while addressing the principle of replacement, reduction and refinement of animal experiments (3rs), humanin vitrobone remodeling models are being developed.Despite increasing safety-, scientific-, and ethical concerns, fetal bovine serum (fbs), a nutritional medium supplement, is still routinely used in these models.To comply with the 3rs and to improve the reproducibility of suchin vitromodels, xenogeneic-free medium supplements should be investigated.Human platelet lysate (h pl) might be a good alternative as it has been shown to accelerate osteogenic differentiation of mesenchymal stromal cells (mscs) and improve subsequent mineralization.However, for a humanin vitrobone model, h pl should also be able to adequately support osteoclastic differentiation and subsequent bone resorption.In addition, optimizing co-culture medium conditions in mono-cultures might lead to unequal stimulation of co-cultured cells.Methods we compared supplementation with 10% fbs vs.10%, 5%, and 2.5% h pl for osteoclast formation and resorption by human monocytes (mcs) in mono-culture and in co-culture with (osteogenically stimulated) human mscs.Results and discussion supplementation of h pl can lead to a less donor-dependent and more homogeneous osteoclastic differentiation of mcs when compared to supplementation with 10% fbs.In co-cultures, osteoclastic differentiation and resorption in the 10% fbs group was almost completely inhibited by mscs, while the supplementation with h pl still allowed for resorption, mostly at low concentrations.The addition of h pl to osteogenically stimulated msc mono- and mc-msc co-cultures resulted in osteogenic differentiation and bone-like matrix formation, mostly at high concentrations.Conclusion we conclude that h pl could support both osteoclastic differentiation of human mcs and osteogenic differentiation of human mscs in mono- and in co-culture, and that this can be balanced by the h pl concentration.Thus, the use of h pl could limit the need for fbs, which is currently commonly accepted forin vitrobone remodeling models.

Journal: Frontiers in immunology (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Age-Adjusted Reference Values for Prostate Specific Antigen - A Systematic Review and Meta-Analysis.

S to systematically evaluates the evidence on ethnic differences in age-adjusted reference values of psa.Materials and methods in concordance with the preferred reporting items for systematic review and meta-analysis statement, a review of english articles using medline, embase and cochrane databases, from inception to december 2019 was conducted.Studies that reported the psa upper reference value as 95th percentile of the cohort distribution, in healthy men aged 40 to 79, were included.Methodological quality was assessed with a modified version of the agency for healthcare research and quality checklist for cross-sectional studies.Results forty-three studies examining 325,514 participants were included in the analysis.These were published between 1993 and 2018.Majority were prospective observational studies and reported the reference values in ten-year age intervals.Only five reports directly compared ethnic differences in psa values.Due to missing data, six studies were not considered in the quantitative synthesis.For the remainder (37/43), heterogeneity in psa reference values was considerable (higgin's index = 99.2%), with age and ethnicity being the sole identified significant contributors.Accordingly, the pooled upper limits for psa reference values were 2.1, 3.2, 4.9 and 6.5 ng/ml for men in their 40 s, 50 s, 60 s, and 70 s, respectively.Conclusion moderate quality evidence suggest that upper psa reference limits increased with age and significant ethnic differences were present.

Journal: Clinical genitourinary cancer (2022)
Publication types: Journal Article;Meta-Analysis;Review;Systematic Review
Protocol for a systematic review of prognostic models for recurrent events in chronic conditions.

Prognostic models for repeated events of the same type are highly useful in predicting when a patient may have a recurrence of a chronic disease or illness.Whilst methods are currently available for analysing recurrent event data in prognostic models, to our knowledge, most are not widely known or applied in a medical setting.As a result, often only the first recurrence is analysed meaning valuable information for multiple recurrences is discarded.Therefore, the aim of this review is to systemically review models for repeated medical events of the same type, to determine what modelling techniques are available and how they are applied.Methods medline will be used as the primary method to search sources.Various databases from the cochrane library and embase will also be searched.Trial registries such as clinicaltrials.gov.uk will be searched, as will registered trials that are ongoing and not yet published.Abstracts submitted to conferences will also be searched, and non-english sources will also be considered.Studies to be included in the review will be decided based on pico guidelines, where the study population and outcomes correspond to this study's aims and target population.The prognostic models used in each study chosen for inclusion in the review will be summarised qualitatively.Discussion as recurrent event data is not widely analysed in prognostic models, the results from this systematic review will identify which methods are available and which are commonly used.It is also unknown if certain methods which will be identified in the review perform better given certain conditions.Therefore, if included studies assess predictive performance, the results of this review could also provide evidence to determine if certain models are better fitting dependant on the event rate of the chronic condition.The results will be used to determine if model selection varies across disease area.The review will also provide an insight into the development of any new methods used for analysing recurrent events.Trial registration the review has been registered on prospero (crd42019116031).

Journal: Diagnostic and prognostic research (2020)
Publication types: Journal Article
Sequential posterior interosseous artery perforator flap for reconstruction of dorsal hand defects.

Dorsal hand defects accompanied by exposure of bones and tendons remain a huge challenge for plastic surgeons.The pedicled perforator flaps have unique advantages in resurfacing the defects.This study aimed to investigate the clinical efficacy of the sequential posterior interosseous artery perforator flap for repairing dorsal hand defects.This study was composed of an anatomical study and clinical application.Anatomically, 30 adult upper limb specimens injected with red latex were dissected, the perforators from the branches of the posterior interosseous artery were observed in the dorsal forearm, and the sequential flap based on them was designed based on the anatomical characteristics.Clinically, nine cases of soft tissue defects on the dorsum of the hand were treated by this flap.Anatomically, the posterior interosseous artery divided into an ascending branch and a descending branch, and the descending branch traveled 1.0 ± 0.3 cm down to divide into the ulnar and radial terminal branches.The ulnar terminal branch presented constantly, and the radial terminal branch had an occurrence rate of 93.33%.Clinically, all flaps survived completely and possessed a soft texture and satisfactory appearance, as well as a nonbloated pedicle, and the donor region had a natural color and appearance with only a linear scar left behind.The sequential posterior interosseous artery perforator flap could become a useful option for repairing dorsal hand defects, as it has the advantages of being easy to perform without sacrificing the main vessels and avoiding donor area skin grafting.

Journal: Clinical anatomy (New York, N.Y.) (2022)
Publication types: Journal Article