syedzayyan/paper_mill_benchmark huggingface dataset:
Alleviation of Inflammation and Oxidative Stress in Pressure Overload-Induced Cardiac Remodeling and Heart Failure via IL-6/STAT3 Inhibition by Raloxifene

Inflammation and oxidative stress are involved in the initiation and progress of heart failure (hf).However, the role of the il6/stat3 pathway in the pressure overload‐induced hf remains controversial.Methods and results.Transverse aortic constriction (tac) was used to induce pressure overload‐hf in c57bl/6j mice.18 mice were randomized into three groups (sham, tac, and tac+raloxifene, n = 6, respectively).Echocardiographic and histological results showed that cardiac hypertrophy, fibrosis, and left ventricular dysfunction were manifested in mice after tac treatment of eight weeks, with aggravation of macrophage infiltration and interleukin‐6 (il‐6) and tumor necrosis factor‐alpha (tnf‐ α ) expression in the myocardium.Tac (four and eight weeks) elevated the phosphorylation of signal transducer and activator of transcription 3 (p‐stat3) and prohibitin2 (phb2) protein expression.Importantly, il‐6/gp130/stat3 inhibition by raloxifene alleviated tac‐induced myocardial inflammation, cardiac remodeling, and dysfunction.In vitro , we demonstrated cellular hypertrophy with stat3 activation and oxidative stress exacerbation could be elicited by il‐6 (25 ng/m l, 48 h) in h9c2 myoblasts.Sustained il‐6 stimulation increased intracellular reactive oxygen species, repressed mitochondrial membrane potential (mmp), decreased intracellular content of atp, and led to decreased sod activity, an increase in i nos protein expression, and increased protein expression of pink1, parkin, and bnip3 involving in mitophagy, all of which were reversed by raloxifene.Conclusion.Inflammation and il‐6/stat3 signaling were activated in tac‐induced hf in mice, while sustained il‐6 incubation elicited oxidative stress and mitophagy‐related protein increase in h9c2 myoblasts, all of which were inhibited by raloxifene.These indicated il‐6/stat3 signaling might be involved in the pathogenesis of myocardial hypertrophy and hf.

Journal: Oxidative Medicine and Cellular Longevity (2021)
Publication types: Research Article;
Adropin Inhibits Vascular Smooth Muscle Cell Osteogenic Differentiation to Alleviate Vascular Calcification via the JAK2/STAT3 Signaling Pathway

Vascular calcification is characterized as the deposition of hydroxyapatite mineral in the form of calcium‐phosphate complexes in the vasculature.Transdifferentiation between vascular smooth muscle cells (vsmcs) and osteoblast‐like cells is considered essential in the progression of vascular calcification.The pathophysiological mechanisms underlying vascular calcification and vsmc osteogenic differentiation remain to be fully elucidated, and the development of novel therapies is required.In the present study, pcr and western blot analysis were conducted to quantify the m rna and protein expression levels of calcification‐associated markers (bone morphogenetic protein 2, alkaline phosphatase, osteoprotegerin, osteocalcin, and runt‐related transcription factor 2) and adropin in vsmcs and rat vascular tissues.The calcification of vsmcs was assessed using alizarin red staining.Moreover, adropin expression levels in vsmcs were analyzed using immunofluorescence.Lentiviral transfection and small interfering rna were used for overexpression and knockdown of adropin in vsmcs, respectively.The results demonstrated that adropin alleviated vascular calcification in vivo.Moreover, adropin also inhibited osteogenic differentiation and the calcification of vsmcs in vitro.Notably, results of the present study revealed that the tyrosine protein kinase jak2 (jak2)/signal transducer and activator of transcription 3 (stat3) signaling pathway played a key role in the aforementioned inhibition.In conclusion, the results of the present study demonstrated that adropin inhibited vsmc osteogenic differentiation to alleviate vascular calcification via the jak2/stat3 signaling pathway.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Acupuncture in the Treatment of Parkinson’s Disease with Sleep Disorders and Dose Response

Acupuncture can effectively improve the sleep state, and most pd patients have sleep disorders.In this study, we used acupuncture to intervene in the sleep state of pdsd, so as to observe the changes and dose effect of acutreatment on pdsd.57 patients with pdsd, during medical treatment, aged 40–70 years were recruited to enroll in this trial.Each participant completed one condition, namely, acutreatment ( n = 30) and sham acutreatment (placebo, stick flat needle on skin, n = 27).The acutreatment was applied for 30 min once a day for a 30‐day observation.Updrsiii scores for motor symptom assessment and sleeping quality were assessed by pdss‐2, ess as well as acti graph.Scale evaluation was made on the first day of admission and the thirtieth day.There were significant differences on all outcome indicators, except updrsiii, on day 30 compared with day 1 ( p &lt; 0.01).Compared with sham acutreatment therapy, acutreatment therapy has better performance in sleep latency, total sleep time, and sleep efficiency ( p &lt; 0.01).Acti graph indicated that sleep efficiency of sham or acutreatment in day 6 was significantly lower than that in day 5 ( p &lt; 0.05 and p &lt; 0.01) and acutreatment in day 7 was significantly lower than that in day 6 ( p &lt; 0.01).The sleep efficiency of acutreatment in days 5, 6, and 7 was significantly higher than that in sham acutreatment ( p &lt; 0.01).Moreover, acutreatment in days 26, 27, and 28 was significantly higher than that in sham acutreatment ( p &lt; 0.01).There was a close correlation between the difference of updrsiii and pdss‐2 ( r = 0.5090, p &lt; 0.05), sleep latency ( r = 0.7201, p &lt; 0.01), tst ( r = −0.6136, p &lt; 0.01), and sleep efficiency ( r = −0.6707, p &lt; 0.01).The sleep condition of pdsd patients can be improved by acupuncture, which can effectively relieve sleep quality, can also be shown by acti graph, and shows a dose‐response relationship.Future research should explore acutreatment with a larger sample size and compare the acutreatment protocol goal formation of the system scheme.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Activation of PTEN/P13K/AKT Signaling Pathway by miRNA-124-3p-Loaded Nanoparticles to Regulate Oxidative Stress Attenuates Cardiomyocyte Regulation and Myocardial Injury

As a common cardiovascular disease, acute myocardial infarction seriously affects the health and life of patients.Mi rnas play an important role in acute myocardial infarction.Based on mi rna obtained from the previous sequencing, this study investigated whether mi rna (mi r)-124-3p-loaded nanoparticles (nps) affect the phenotype of the acute myocardial infarction (ami) rat.Nano-mi r-124-3p decreased the myocardial infarction area, improved the myocardial tissue structure, and increased the degree of fibrosis.Nano-mi r-124-3p decreased apoptosis and the expression of cleaved caspase 3, indicating its role in protecting and repairing the myocardium.To further verify the action mechanism of mi rna, a potential target gene of mi r-124-3p, pten was identified by starbase and further confirmed using double luciferase assays.Following cotransfection of nano-mi r-124-3p and pten, the areas of tissue structure damage, myocardial infarction, and fibrosis were substantially elevated.The expression of cleaved caspase 3 and the apoptosis rate in the nano-mi r-124-3p and pten cotransfection group was also significantly increased.Bioinformatics analysis revealed that mi rna-124-3 may regulate oxidative stress injury by targeting pten.Taken together, mi r-124-3p could protect and repair myocardial tissues through targeting pten.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Activating IL-6/STAT3 Enhances Protein Stability of Proteasome 20S α+ β in Colorectal Cancer by miR-1254

A widely recognized feature of colorectal cancer (crc) is an increase in cytokine levels, which result in an inflammatory environment in the tumor.Interleukin‐6 (il‐6) is a robust protumor cytokine.Several studies suggest that il‐6 plays a role in the development of tumors.Most intracellular protein breakdown occurs in eukaryotes via the ubiquitin‐proteasome pathway; this mechanism may also be involved in cancer pathogenesis.The tumor tissues and paracancerous tissues were collected from 90 patients with colorectal cancer.The expressions of p stat3, proteasome 20s α + β , mi r‐1254, and psmd1 in tissues were detected by immunohistochemistry, elisa, and q rt‐pcr, and the effects of p stat3 and proteasome 20s α + β expressions on the survival of patients were studied.Hct116 and hct116‐r cells were cultured and added il‐6, ag490, stat3 plasmid, or overexpression/knockdown of mi r‐1254 in cells.Immunofluorescence, western blot, q rt‐pcr, double luciferase gene reporter assay, and flow cytometry were used to detect the expression of p stat3, stat3, proteasome 20s α + β , mi r‐1254, and psmd1 and cell cycle.The nude mouse xenograft model was constructed and divided into 3 groups: pbs group, il‐6 treatment group, and il‐6+mi r‐1254 mimic group.After 28 days, the tumor tissues were collected, and the expressions of mi r‐1254, p stst3, proteasome 20s α + β , and psmd1 in the tissues were detected by q rt‐pcr and immunohistochemistry, respectively.Our study discovered that the level of proteasome 20s α + β had a strong connection with p stat3 in crc patients.They were also linked to the development and clinical outcome of crc.In addition, we found that il‐6 dramatically increased the expression of proteasome 20s α + β and p stat3; however, it did not affect the proteasome 20s α + β m rna synthesis.Circulating proteasome concentration correlated with tumor tissue proteasome 20s α + β .Stat3 could occupy the mi r‐1254 promoter to inhibit transcription, and it could suppressed mi r‐1254 which targeted psmd10, promoting proteasome 20s α + β protein stability.This is a prospective target for developing a new colorectal cancer therapy strategy.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Ablation of Basic Leucine Zipper Transcription Factor ATF-Like Potentiates Estradiol to Induce Atopic Dermatitis

Atopic dermatitis (ad) is an inflammatory and immune skin disorder.Basic leucine zipper transcription factor atf-like (batf) plays a key role in regulating the differentiation and functions of lymphocytes.However, the mechanism underlying the transcriptional regulation of batf on ad is still not well understood.Methods.Batf knockout (batf–/–) and c57bl/6(b6) mice were used for the development of spontaneous dermatitis.17β-estradiol was injected intraperitoneally to induce ad.The lesioned tail skin of the mice was stained with hematoxylin and eosin to analyze the pathological characteristics.Impaired skin barrier function was assessed by measuring the transepidermal water loss (tewl).The skin epithelial barrier indicators and cytokine m rna levels were quantified by real-time quantitative pcr.The total serum immunoglobulin e (ig e) levels were measured by enzyme-linked immunosorbent assay (elisa).T lymphocytes were analyzed using flow cytometry.Results.Ablation of batf led to the spontaneous development of ad only in female mice and not in male mice.Batf deletion led to elevated serum levels of ig e and increased infiltration of eosinophils, neutrophils, and lymphocytes and promoted cytokine production including il-4, il-22, il-1β, ifn-γ, and tnf-α in the lesioned tail skin of the mice.The m rna expression levels of filaggrin and loricrin significantly decreased, while s100a8 and s100a9 increased in female batf–/– mice.Batf-deficient female mice were found to increase proliferation and il-5 production by skin-infiltrating cd4+ t cells which implies th2 activation.Moreover, ad was successfully induced only in the estradiol-treated batf-deficient male mice and not in wt male mice.Estradiol enhanced the allergic and immunological responses to dermatitis primarily by triggering th2-type immune responses via enhanced serum ig e and inflammatory cytokine levels in the male batf–/– mice.Conclusion.The study concluded that batf potentiates estradiol to induce mouse atopic dermatitis via potentiating inflammatory cytokine releases and th2-type immune responses and may have important clinical implications for patients with ad.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
A Task Allocation Algorithm for Coal Mine Mobile Crowd Sensing Based on Weighted Undirected Graph

Existing applications of mining intelligent mobile terminals are all for the sensing of individual terminals, without considering the group attributes of terminal carriers and the cooperation opportunities brought by terminal movement, which is unable to complete large-scale and complex sensing tasks.In this article, mobile crowd sensing and mine safety monitoring are combined to construct the mobile crowd sensing network for coal mine.Aiming at the task allocation problem, a task allocation mechanism based on a weighted undirected graph is proposed.First, considering the similarity of task completion location and time and quality of data collected, the reputation evaluation model of miners is designed.In order to optimize the reputation of miners participating in the task, an emergency task allocation algorithm based on the weighted undirected graph is proposed.Second, based on the fatigue degree of miners and the residual energy of intelligent mobile terminals, the miner status evaluation model is constructed, which is combined with the reputation model to design a nonemergency task allocation algorithm, aiming at further optimizing the task allocation results.The simulation results show that the proposed algorithm has better performance in allocation time for sensing task, task allocation success rate, average reputation, and status value of miners.

Journal: Security and Communication Networks (2021)
Publication types: Research Article;
A Retrospective Study on the Time in Range of Blood Glucose and Type 2 Diabetic Peripheral Neuropathy

Time in range (tir) is one of the basic indicators to assess glycemic control.In this study, the tir of dpn patients was used as the observation index to further evaluate the correlation between tir and dpn, so as to provide new ideas for preventing the occurrence of dpn and delaying its disease progression.Methods .A total of 120 patients with t2dm (t2dm) who were hospitalized in the endocrinology department of our hospital from october 2018 to february 2020 were included and divided into two groups according to whether the nerve conduction velocity was normal or not, the diabetic peripheral neuropathy group (dpn) and the other groups.No diabetic peripheral neuropathy group (ndpn).According to the corresponding inclusion and exclusion criteria, the baseline data were recorded, and test indicators such as homocysteine and blood lipids were collected at the same time, and tir was collected by a transient blood glucose meter.To explore the relationship between tir and other indicators and peripheral neuropathy in t2dm.Results .A total of 120 t2dm patients participated in the study, including 82 in the dpn group and 38 in the ndpn group.There were no statistically significant differences in basic indicators such as age, height, and weight between the two groups.Glycated hemoglobin (hb a1c) and homocysteine (hcy) in dpn group were higher than those in ndpn group, while tir and hdl‐c were lower than those in ndpn group ( p &lt; 0.05).Logistic regression analysis showed that hb a1c and hcy were risk factors for dpn, and tir and hdl‐c were protective factors for dpn, with statistical significance ( p &lt; 0.05).The prediction results of tir, hcy, hdl‐c, and hb a1c on diabetic peripheral neuropathy were analyzed by roc curve, and the prediction results of the five variables were all statistically significant ( p &lt; 0.05) and have a better prediction effect.Conclusion .(1) the results of tir level suggest that the longer the blood sugar is in the good control range, the more beneficial it is to reduce the occurrence of dpn.(2) tir and hdl‐c are protective factors for dpn, and hb a1c and hcy are risk factors for dpn.(3) the results of roc curve analysis showed that tir, hcy, hb a1c, and hdl‐c had a good predictive effect on the occurrence of dpn.

Journal: BioMed Research International (2022)
Publication types: Research Article;
A Modified Deep Learning Framework for Arrhythmia Disease Analysis in Medical Imaging Using Electrocardiogram Signal

Arrhythmias are anomalies in the heartbeat rhythm that occur occasionally in people’s lives.These arrhythmias can lead to potentially deadly consequences, putting your life in jeopardy.As a result, arrhythmia identification and classification are an important aspect of cardiac diagnostics.An electrocardiogram (ecg), a recording collecting the heart’s pumping activity, is regarded the guideline for catching these abnormal episodes.Nevertheless, because the ecg contains so much data, extracting the crucial data from imagery evaluation becomes extremely difficult.As a result, it is vital to create an effective system for analyzing ecg’s massive amount of data.The ecg image from ecg signal is processed by some image processing techniques.To optimize the identification and categorization process, this research presents a hybrid deep learning‐based technique.This paper contributes in two ways.Automating noise reduction and extraction of features, 1d ecg data are first converted into 2d pictures.Then, based on experimental evidence, a hybrid model called cnnlstm is presented, which combines cnn and lstm models.We conducted a comprehensive research using the broadly used mit_bih arrhythmia dataset to assess the efficacy of the proposed cnn‐lstm technique.The results reveal that the proposed method has a 99.10 percent accuracy rate.Furthermore, the proposed model has an average sensitivity of 98.35 percent and a specificity of 98.38 percent.These outcomes are superior to those produced using other procedures, and they will significantly reduce the amount of involvement necessary by physicians.

Journal: BioMed Research International (2022)
Publication types: Research Article;
A Feasible Multimodal Photoacoustic Imaging Approach for Evaluating the Clinical Symptoms of Inflammatory Arthritis

Numerous traditional medical imaging methods, including computed tomography with x‐rays, positron emission tomography (pet), and magnetic resonance imaging (mri), are utilized frequently in medical settings to screen for illnesses, diagnose patients, and track the effectiveness of treatments.When examining bone protrusions, ct is preferred over mri for scanning connective tissue.Although the picture quality of pet is inferior to that of ct and mr, it is outstanding for detecting the molecular markers and metabolic functions of illnesses.To give high‐resolution structural pictures and improved ailment sensitivity and specificity within another image, multimodal data and substantial therapeutic influence on advanced diagnostics and therapeutics have been used.The goal was to evaluate the clinical significance of multimodal photoacoustic/ultrasound (pa/us) articular imaging scoring, a cutting‐edge image technique that may show the microvessels and oxygen levels of rheumatoid arthritis‐related inflamed joints (ra).The pa/us imaging technology analyzed seven tiny joints.The pa and power doppler (pd) impulses were semiquantified using a 0–3 grading scale, and the averages of the pa and pd scores for the seven joints are computed.Three pa+so 2 types were found determined by the relative oxygen levels (so 2 ) measurements of the affected joints.Researchers evaluated the relationships between the disease activity ratings and the pa/us imaging ratings.The pa scores and medical ratings that reflect the extent of the pain have strong relationships with each other, as do the pa+so 2 combinations.Pa may be clinically useful in assessing ra.Thus, the research evaluated the clinical symptoms of inflammatory arthritis using a multimodal photoacoustic image process.

Journal: BioMed Research International (2022)
Publication types: Research Article;
A Deep Learning Framework for Earlier Prediction of Diabetic Retinopathy from Fundus Photographs

Diabetic patients can also be identified immediately utilizing retinopathy photos, but it is a challenging task.The blood veins visible in fundus photographs are used in several disease diagnosis approaches.We sought to replicate the findings published in implementation and verification of a deep learning approach for diabetic retinopathy identification in retinal fundus pictures.To address this issue, the suggested investigative study uses recurrent neural networks (rnn) to retrieve characteristics from deep networks.As a result, using computational approaches to identify certain disorders automatically might be a fantastic solution.We developed and tested several iterations of a deep learning framework to forecast the progression of diabetic retinopathy in diabetic individuals who have undergone teleretinal diabetic retinopathy assessment in a basic healthcare environment.A collection of one‐field or three‐field colour fundus pictures served as the input for both iterations.Utilizing the proposed drnn methodology, advanced identification of the diabetic state was performed utilizing he detected in an eye’s blood vessel.This research demonstrates the difficulties in duplicating deep learning approach findings, as well as the necessity for more reproduction and replication research to verify deep learning techniques, particularly in the field of healthcare picture processing.This development investigates the utilization of several other deep neural network frameworks on photographs from the dataset after they have been treated to suitable image computation methods such as local average colour subtraction to assist in highlighting the germane characteristics from a fundoscopy, thus, also enhancing the identification and assessment procedure of diabetic retinopathy and serving as a skilled guidelines framework for practitioners all over the globe.

Journal: BioMed Research International (2022)
Publication types: Research Article;