syedzayyan/paper_mill_benchmark huggingface dataset:
Effect of IMB Model Combined with Spousal Support Breastfeeding Intervention on PBSES Score and Breastfeeding Rate of Primipara with Chronic Hepatitis B Virus Infection

To analyze the effects of the info‐motivation‐behavior skills (imb) model combined with spousal support breastfeeding intervention on breastfeeding self‐efficacy scale (pbses) scores and breastfeeding rate of primiparas with chronic hepatitis b virus (hbv) infection.Methods .Seventy‐four first‐term pregnant women and 74 of their spouses were selected as the traditional control group by the convenience sampling method from july to september 2021 in obstetrics department of shenzhen third people’s hospital.74 pregnant women with their first child and 74 spouses who had their first child checked during october to december 2021 were classified as the imb model group.The traditional control group was applied with conventional intervention management mode, and the imb model group was applied with intervention management mode based on imb theory on the basis of the traditional control group.The self‐efficacy scores of breastfeeding before and after intervention during pregnancy and during hospitalization were compared between the two groups, and the self‐efficacy scores of paternal support for breastfeeding were compared.The exclusive breastfeeding rate of infants within 6 months and the maternal breastfeeding knowledge level of the two groups were compared, and the correlation between maternal breastfeeding self‐efficacy score and feeding knowledge level was analyzed.Results .After pregnancy intervention, pbses and fbses‐sf scores were significantly increased in both groups, and scores of scales in the imb model group increased significantly than the traditional control group (all p &lt; 0.05).The bses‐sf and fbses‐sf scores of the imb model group increased significantly than the traditional control group at 3 days after delivery and at discharge ( p &lt; 0.05), and the scores of each scale at discharge in both groups increased significantly than those at 3 days after delivery ( p &lt; 0.05).The rate of exclusive breastfeeding in the imb model group was 94.59% (70/74), and that in the traditional control group was 78.38% (58/74).There was a significant difference ( χ 2 = 8.325, p = 0.004).At discharge, the score of maternal breastfeeding knowledge increased significantly in both groups, and the score of the imb model group increased significantly than that of the traditional control group (all p &lt; 0.05).Pearson correlation coefficient was used to analyze the correlation between pbses score, fbses‐sf score, and maternal feeding knowledge level, which showed positive correlation (all p &lt; 0.05).Conclusion .The self‐efficacy of prenatal breastfeeding in pregnant women with hbv is low, and the application of the imb model combined with the intervention mode of spy‐supported breastfeeding has positive effects on the improvement of maternal breastfeeding efficiency, breastfeeding health knowledge level, and postpartum breastfeeding rate, which is worthy of clinical promotion and application.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Effect of Evodiamine on Collagen-Induced Platelet Activation and Thrombosis

Evodia rutaecarpa has multiple pharmacological effects and is widely used in the prevention and treatment of migraine, diabetes, cardiovascular disease, cancer, and other chronic diseases; however, the pharmacological effects of its active compound evodiamine (evo) have not been thoroughly investigated.The purpose of this study was to investigate the effects of evo on antiplatelet activation and thrombosis.We discovered that evo effectively inhibited collagen‐induced platelet activation but had no effect on platelet aggregation caused by activators such as thrombin, adp, and u46619.Second, we found that evo effectively inhibited the release of platelet granules induced by collagen.Finally, evodiamine inhibits the transduction of the sfks/syk/akt/plc γ 2 activation pathway in platelets.According to in vivo studies, evo significantly prolonged the mesenteric thromboembolism induced by ferric chloride and had no discernible effect on the coagulation function of mice.In conclusion, the antiplatelet and thrombotic effects of evo discovered in this study provide an experimental basis for the investigation of the pharmacological mechanisms of evo and the development of antiplatelet drugs.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Effect of Erdosteine on Middle Ear Effusion in Rats by Mediating TLR4 Signaling Pathway

The study aimed to investigate the effect of erdosteine on middle ear effusion in rats through mediating the toll‐like receptor 4 (tlr4) signaling pathway.Rats were injected with endotoxin to prepare the model of acute secretory otitis media (som).Then, they were divided into an acute som model group (model group, n = 15) and erdosteine treatment group (18 mg/kg, gavage, treatment group, n = 15).Besides, a normal group ( n = 15) was set up.Two weeks later, routine biochemical indicators such as aspartate aminotransferase (ast) and alkaline phosphatase (alp) were detected.The inflammatory effusion due to otitis media was scored.The content of myeloperoxidase (mpo), matrix metalloproteinase (mmp), and tumor necrosis factor‐beta (tnf‐ β ) in middle ear lavage fluid was detected via enzyme‐linked immunosorbent assay (elisa).Additionally, histomorphological changes were observed with the help of hematoxylin‐eosin (he) staining, and quantitative reverse transcription‐polymerase chain reaction (q rt‐pcr) and western blotting assays were carried out to measure the expression levels of tlr4 pathway genes and proteins as well as the messenger ribonucleic acid (m rna) expression levels of key factors for otitis media (mucin 2 (muc2) and muc5a).In the model group, the levels of ast, alp, and glutamic‐pyruvic transaminase (gpt) were significantly increased ( p &lt; 0.05).Besides, the content of mpo, mmp, and tnf‐ β was overtly raised in the model group ( p &lt; 0.05), while it was notably lowered in the treatment group ( p &lt; 0.05).In the treatment group, the cilia were slightly swollen, and inflammatory cells were fewer.The m rna levels of muc2, muc5a, and pathway genes tlr4 and c‐jun n‐terminal kinase (jnk) were elevated in the model group.In addition, the protein assay results revealed that the protein levels of tlr4 and jnk were evidently increased in the model group.Erdosteine can treat the middle ear effusion in rats by repressing the activation of the tlr4 signaling pathway.

Journal: BioMed Research International (2021)
Publication types: Research Article;
Effect of Dihuang Yinzi on Inflammatory Response in Cerebral Ischemia-Reperfusion Model Rats by Regulating Gut Microbiota

Dihuang yinzi, as a classical chinese medicine prescription, plays an important role for the treatment of ischemic stroke.Gut microbiota play a functional role for the expression of proinflammatory cytokines and anti‐inflammatory cytokines, which further affect central nervous system and change brain function.Our research confirmed that dihuang yinzi can exert brain protection by inhibiting inflammatory reaction.Dihuang yinzi can significantly decrease the contents of interleukin‐6 (il‐6), tumor necrosis factor‐ α (tnf‐ α ), and interleukin‐17 (il‐17) in brain, serum, and colon tissues and increase the contents of transforming growth factor‐ β (tgf‐ β ) and interleukin‐10 (il‐10) in cerebral ischemia‐reperfusion model rats.The results of 16s r rna high‐throughput sequencing showed that dihuang yinzi had a significant effect on microbiome in rats.The firmicutes, bacteroidetes, and proteobacteria were dominant in dihuang yinzi group.The content of firmicutes increased with the increase of dosage of dihuang yinzi.Especially, the content of actinomycetes in the high‐dose group was higher than other groups.At the genus level, the number of bacteroides in the antibiotic groups was significantly higher than that in the other treatment groups.The results suggest that dihuang yinzi may play important roles in treatment of ischemic stroke by regulating the gut microbiota and the inflammatory reaction in the colon tissues, serum, and brain of the model rats, to verify the scientific nature of this prescription in relieving brain inflammatory reaction and brain injury by this way and to reveal the brain‐gut related mechanism of dihuang yinzi in treating ischemic stroke.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Hospital Intelligent Power Operation and Maintenance Information Evaluation with the Long and Short Memory Neural Network

The invention describes a deep learning‐based technique for monitoring power grid information operation and maintenance.Based on the time series data information in the power grid information operation and maintenance monitoring system, this method obtains the cleaned time series data through appropriate data preprocessing technology.This also uses the long‐term and short‐term memory neural network to realize the prediction function of the time series data to be detected.The reason behind is to construct the normal behavior model of the time series.Additionally, the control chart based on an exponentially weighted moving average is employed to determine whether the time series to be discovered contains any anomalous and abnormal phenomena.In the area of power grid information operation and maintenance monitoring, the method of invention is designed to counter anomaly.This phenomenon is universal in nature and has significant scientific importance for guiding treatment after the anomaly is discovered.Additionally, it guards against serious faults that the abnormality might bring about.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Ligustrazine inhibits the proliferation and migration of ovarian cancer cells via regulating miR-211

Ovarian cancer (oc) is a commonly diagnosed female cancer.Ligustrazine (lsz), a natural compound, has been reported to exert anti-cancer activity, although the mechanisms underlying the anti-cancer effects are not clear.The present study investigated the impact of lsz on cell proliferation and migration by regulating micro rna-211 (mi r-211) expression using the human ovarian cancer sk-ov-3 and ovcar-3 cell lines.Oc cells were treated with 0, 0.5, 1, and 2 m m lsz, and quantitative real-time pcr was utilized to measure mi r-211 levels in sk-ov-3 and ovcar-3 cells with different treatment.Moreover, to further confirm the roles of mi r-211 in lsz induced anti-tumor effects, mi r-211 expression was inhibited by transfection of mi r-211 inhibitors in sk-ov-3 cells.Cell proliferation of transfected cells was evaluated using the cck-8 and colony formation assay.The scratch assay was employed to assess cell migration and transwell assay was performed for evaluating the cell invasion.Protein levels of epithelial–mesenchymal transition (emt) markers were determined by western blotting.We found that lsz inhibited the viability, proliferation, migration and invasion ability of sk-ov-3 and ovcar-3 cells in a dose-dependent manner; moreover, lsz could significantly increase the expression of mi r-211 in both sk-ov-3 and ovcar-3, and knockdown of mi r-211 in sk-ov-3 cells partially abrogated the anti-tumor behavior of lsz by promoting the viability, proliferation, migration, invasion and emt of sk-ov-3 cells.Thus, we found that lsz can inhibit the proliferation and migration of oc cells via regulating mi r-211.Our study suggests that lsz might be a potential and effective treatment for oc.

Journal: Bioscience Reports (2021)
Publication types: Research Article;
Naringin protects endothelial cells from apoptosis and inflammation by regulating the Hippo-YAP Pathway

Atherosclerosis is the primary cause of several cardiovascular diseases.Oxidized low-density lipoprotein (ox-ldl)-induced apoptosis, endothelial–mesenchymal transition (end mt), and inflammation are crucial for the progression of cardiovascular diseases, including atherosclerosis.Naringin, a major compound from tomatoes, grapefruits, and related citrus, reportedly exhibits potential protective effects during atherosclerosis development; however, its effect on ox-ldl-induced human umbilical vein endothelial cell (huvec) damage remains unknown.In the present study, we investigated the anti-apoptotic and anti-inflammatory activities of naringin against ox-ldl-induced endothelial cells, and the underlying mechanism.Naringin pretreatment significantly and concentration-dependently inhibited ox-ldl-induced cell injury and apoptosis.Additionally, naringin restored endothelial barrier integrity by preventing ve-cadherin disassembly and f-actin remodeling, and down-regulated pro-inflammatory factors like il-1β, il-6, and il-18, in the huvecs.We also demonstrated that naringin treatment restored ox-ldl-induced yap (yes-associated protein) down-regulation, given the yap-sh rna attenuated cytoprotective effect of naringin on ox-ldl-induced endothelial cell injury and apoptosis.Collectively, our data indicate that naringin reversed ox-ldl-triggered huvec apoptosis, end mt, and inflammation by inhibiting the yap pathway.Therefore, naringin may have a therapeutic effect on endothelial injury-related disorders.

Journal: Bioscience Reports (2020)
Publication types: Research Article;
Modulation of miR-146a/complement factor H-mediated inflammatory responses in a rat model of temporal lobe epilepsy

Increasing evidence supports the involvement of inflammatory and immune processes in temporal lobe epilepsy (tle).Mi rnas represent small regulatory rna molecules that have been shown to act as negative regulators of gene expression controlling different biological processes, including immune system homoeostasis and function.We investigated the expression and cellular distribution of mi rna-146a (mi r-146a) in a rat model of tle.Prominent up-regulation of mi r-146a activation was evident in 1 week after status epilepticus (se) and persisted in the chronic phase.The predicted mi r-146a's target complement factor h (cfh) m rna and protein expression was also down-regulated in tle rat model.Furthermore, transfection of mi r-146a mimics in neuronal and glial cells down-regulated cfh m rna and protein levels respectively.Luciferase reporter assays demonstrated that mi r-146a down-regulated cfh m rna expression via 3′-utr pairing.Down-regulating mi r-146a by intracerebroventricular injection of antagomir-146a enhanced the hippocampal expression of cfh in tle model and decreased seizure susceptibility.These findings suggest that immunopathological deficits associated with tle can in part be explained by a generalized mi r-146a-mediated down-regulation of cfh that may contribute to epileptogenesis in a rat model of tle.

Journal: Bioscience Reports (2016)
Publication types: Research Article;
Effect of Carrying out Continuous Nursing Based on Mobile Platform on the Life of Children with Leukemia after Discharge from Hospital

To analyze the effect of carrying out continuous nursing based on mobile platform on the life of children with leukemia after discharge.Methods .A total of 104 children diagnosed with leukemia admitted in pediatric internal medicine, hainan provincial people’s hospital, from september 2019 to august 2020 were randomly divided into two groups, observation group and control group, with 52 cases in each group.For the control group, routine follow‐up was used for continuous nursing after discharge from hospital, and the observation group was treated with continuous nursing based on mobile platform on the basis of routine follow‐up nursing after discharge from hospital.Results .When discharged from hospital, there was no significant difference in sds and sas scores between the two groups ( p &gt; 0.05).After 8 weeks of discharge, sds and sas scores in both groups were significantly decreased, and sds and sas scores in the observation group were significantly lower than those in the control group, with statistical significance ( p &lt; 0.05).After 8 weeks of discharge, the cancer‐related fatigue score of the observation group was significantly lower than that of the control group, and the difference was statistically significant ( p &lt; 0.05).When discharged from hospital, there was no significant difference in quality of life between two groups ( p &gt; 0.05).Eight weeks after discharge, the quality of life in both groups was significantly improved, and the quality of life in the observation group was significantly better than that in the control group; the difference was statistically significant ( p &lt; 0.05).The occurrence of adverse reactions in the observation group was significantly lower than that in the control group, and the difference was statistically significant ( p &lt; 0.05).Conclusion .After children with leukemia were discharged from hospital, medical staff used mobile platform to carry out continuous nursing for them, which could relieve the negative emotions of children, reduce the incidence of adverse reactions, and improve the quality of life.This kind of intervention had promotion value.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Downregulation of microRNA-15b-5p Targeting the Akt3-Mediated GSK-3β/β-Catenin Signaling Pathway Inhibits Cell Apoptosis in Parkinson’s Disease

Parkinson’s disease (pd) is an incurable progressive disorder resulting from neurodegeneration, and apoptosis is considered a dominant mechanism underlying the process of neurodegeneration.Micro rnas (mi rnas), which are small and noncoding rnas involved in many a biological process like apoptosis and regulation of gene expressions, have been found in postmortem brain samples of patients with pd, as well as in vitro and in vivo models of pd.To explore the impact of mi r‐15b‐5p and akt3 on apoptosis in the progression of pd, the method of quantitative reverse transcription polymerase chain reaction (q rt‐pcr) was employed, and the analysis result showed upregulated expression of mi r‐15b‐5p and downregulated expression of akt3 in the serum of pd patients, mpp + ‐induced sh‐sy5y cells, and the brain tissues of mptp‐induced mice.Meanwhile, the dual‐luciferase reporter assay was used to demonstrate the regulator‐target interaction between mi r‐15b‐5p and akt3; flow cytometry and spectrophotometry revealed that transfection of mi r‐15b‐5p mimic and si‐akt3 increased the rate of apoptosis and caspase‐3 activity, whereas transfecting the mi r‐15b‐5p inhibitor and akt3‐overexpression plasmid repressed the rate of apoptosis and caspase‐3 activity in the mpp + ‐induced sh‐sy5y cell model and the mptp‐induced mouse model.Additionally, analysis of western blotting (wb) assays in vivo and in vitro revealed that proapoptosis proteins (bax, caspase‐3, gsk‐3 β , and β ‐catenin) showed markedly upregulated expression in the mi r‐15b‐5p inhibitor and si‐akt3‐overexpression groups, while the expression of an antiapoptosis gene (i.e., bcl2) was downregulated.These analysis results indicate that downregulation of mi r‐15b‐5p by targeting the akt3‐mediated gsk‐3 β / β ‐catenin signaling pathway would repress cell apoptosis in pd in vivo and in vitro .It is expected that the research findings would help find new therapeutic targets for treatment of pd.

Journal: BioMed Research International (2021)
Publication types: Research Article;
Dioscorea nipponica Makino Relieves Ovalbumin-Induced Asthma in Mice through Regulating RKIP-Mediated Raf-1/MEK/MAPK/ERK Signaling Pathway

Dioscorea nipponica makino (dnm) is a traditional herb with multiple medicinal functions.This study is aimed at exploring the therapeutic effects of dnm on asthma and the underlying mechanisms involving rkip‐mediated mapk signaling pathway.Methods .An ovalbumin‐induced asthma model was established in mice, which was further administrated with dnm and/or locostatin (rkip inhibitor).Elisa was performed to detect the serum titers of ova‐ig e and ova‐ig g1, bronchoalveolar lavage fluid (balf) levels of inflammation‐related biomarkers, and tissue levels of oxidative stress‐related biomarkers.The expression of rkip was measured by quantitative real‐time pcr, western blot, immunohistochemistry, and immunofluorescence.He staining was used to observe the pathological morphology of lung tissues.The protein expression of mapk pathway‐related proteins was detected by western blot.Results .Compared with the controls, the model mice exhibited significantly higher serum titers of ova‐ig e and ova‐ig g1, balf levels of il‐6, il‐8, il‐13, tgf‐ β 1, and mcp‐1, tissue levels of mda and ros, lower balf levels of il‐10 and ifn‐ γ , and tissue level of gsh.Dnm relieved the allergic inflammatory response and oxidative stress in the model mice.Dnm also recovered the downregulation of rkip and the pathological injury of lung tissues in asthma mice.In addition, the raf‐1/mek/mapk/erk pathway in the model mice was blocked by dnm.Silencing of rkip by locostatin weakened the relieving effects of dnm on asthma through activating the raf‐1/mek/mapk/erk pathway.Conclusion .Dnm relieves asthma via blocking the raf‐1/mek/mapk/erk pathway that mediated by rkip upregulation.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Dietary Quercetin Supplementation Attenuates Diarrhea and Intestinal Damage by Regulating Gut Microbiota in Weanling Piglets

Antioxidant polyphenols from plants are potential dietary supplementation to alleviate early weaning‐induced intestinal disorders in piglets.Recent evidences showed polyphenol quercetin could reshape gut microbiota when it functioned as anti‐inflammation or antioxidation agents in rodent models.However, the effect of dietary quercetin supplementation on intestinal disorders and gut microbiota of weanling piglets, along with the role of gut microbiota in this effect, both remain unclear.Here, we determined the quercetin’s effect on attenuating diarrhea, intestinal damage, and redox imbalance, as well as the role of gut microbiota by transferring the quercetin‐treated fecal microbiota to the recipient piglets.The results showed that dietary quercetin supplementation decreased piglets’ fecal scores improved intestinal damage by increasing tight junction protein occludin, villus height, and villus height/crypt depth ratio but decreased crypt depth and intestinal epithelial apoptosis (tunel staining).Quercetin also increased antioxidant capacity indices, including total antioxidant capacity, catalase, and glutathione/oxidized glutathione disulfide but decreased oxidative metabolite malondialdehyde in the jejunum tissue.Fecal microbiota transplantation (fmt) from quercetin‐treated piglets had comparable effects on improving intestinal damage and antioxidative capacity than dietary quercetin supplementation.Further analysis of gut microbiota using 16s r dna sequencing showed that dietary quercetin supplementation or fmt shifted the structure and increased the diversity of gut microbiota.Especially, anaerobic trait and carbohydrate metabolism functions of gut microbiota were enriched after dietary quercetin supplementation and fmt, which may owe to the increased antioxidative capacity of intestine.Quercetin increased the relative abundances of fibrobacteres, akkermansia muciniphila , clostridium butyricum , clostridium celatum , and prevotella copri but decreased the relative abundances of proteobacteria, lactobacillus coleohominis , and ruminococcus bromii .Besides, quercetin‐shifted bacteria and carbohydrate metabolites short chain fatty acids were significantly related to the indices of antioxidant capacity and intestinal integrity.Overall, dietary quercetin supplementation attenuated diarrhea and intestinal damage by enhancing the antioxidant capacity and regulating gut microbial structure and metabolism in piglets.

Journal: Oxidative Medicine and Cellular Longevity (2021)
Publication types: Research Article;