syedzayyan/paper_mill_benchmark huggingface dataset:
Intraoperative Neuromonitoring and Optical Magnification in the Prevention of Recurrent Laryngeal Nerve Injuries during Total Thyroidectomy.

And objectives: recurrent laryngeal nerve (rln) paralysis is a fearful complication during thyroidectomy.Intraoperative neuromonitoring (ionm) and optical magnification (om) facilitate rln identification and dissection.The purpose of our study was to evaluate the influence of the two techniques on the incidence of rln paralysis and determine correlations regarding common outcomes in thyroid surgery.Materials and methods: two equally sized groups of 50 patients who underwent total thyroidectomies were examined.In the first group (om), only surgical binocular loupes (2.5×−4.5×) were used during surgery, while in the second group (ionm), the intermittent nim was applied.Results: both the operative time and the length of hospitalization were shorter in the om group than in the ionm group (median 80 versus 100 min and median 2 versus 4 days, respectively) (p 0.05).No permanent rln paralysis or hypoparathyroidism have been reported.Conclusions: despite some limitations, our study is the first to compare the use of ionm with om alone in the prevention of rln injuries.The risk of recurrent complications remains comparable and both techniques can be considered valid instruments, especially if applied simultaneously by surgeons.

Journal: Medicina (Kaunas, Lithuania) (2022)
Publication types: Journal Article
Vestibulo-ocular reflex involvement in childhood-onset multiple sclerosis.

Multiple sclerosis (ms), an autoimmune demyelinating disease of the central nervous system, is an important cause of disability in young adults.The purpose of this cross-sectional study was to evaluate the vestibular system with video head impulse test (v hit) and determine the impairment of the vestibulo-ocular reflex (vor) in childhood-onset ms.Methods the study group, 20 persons with ms (pw ms) with onset before 18 years of age (6 m, 14 f; mean age 19.06 ± 1.66) and the control group, 20 healthy, age- and sex-matched individuals were retrieved from v hit recordings.The mean age of ms onset in the study group was 14.60 ± 1.53 years.The vor pathway was evaluated using v hit.Results the median vor gains of right anterior (1.00), left lateral (0.96) and left posterior (0.91) semicircular canals were significantly lower in the pw ms group than those of the healthy control group (1.05, 1.00, 0.98 respectively, p < 0.05).Four of pw ms (20%) had abnormal vor gains.The pw ms with dizziness had significantly lower vor gains (median 0.91) compared with pw ms without dizziness (median 1.01, p < 0.05).Conclusion this study demonstrates vestibulo-ocular system can be affected in patients with childhood-onset ms and suggests using v hit especially in the follow-up of pw ms with dizziness.

Journal: Multiple sclerosis and related disorders (2020)
Publication types: Journal Article
Molecular Dynamics Simulation Studies on the Aggregation of Amyloid-β Peptides and Their Disaggregation by Ultrasonic Wave and Infrared Laser Irradiation.

Alzheimer's disease is understood to be caused by amyloid fibrils and oligomers formed by aggregated amyloid-β (aβ) peptides.This review article presents molecular dynamics (md) simulation studies of aβ peptides and aβ fragments on their aggregation, aggregation inhibition, amyloid fibril conformations in equilibrium, and disruption of the amyloid fibril by ultrasonic wave and infrared laser irradiation.In the aggregation of aβ, a β-hairpin structure promotes the formation of intermolecular β-sheet structures.Aβ peptides tend to exist at hydrophilic/hydrophobic interfaces and form more β-hairpin structures than in bulk water.These facts are the reasons why the aggregation is accelerated at the interface.We also explain how polyphenols, which are attracting attention as aggregation inhibitors of aβ peptides, interact with aβ.An md simulation study of the aβ amyloid fibrils in equilibrium is also presented: the aβ amyloid fibril has a different structure at one end from that at the other end.The amyloid fibrils can be destroyed by ultrasonic wave and infrared laser irradiation.The molecular mechanisms of these amyloid fibril disruptions are also explained, particularly focusing on the function of water molecules.Finally, we discuss the prospects for developing treatments for alzheimer's disease using md simulations.

Journal: Molecules (Basel, Switzerland) (2022)
Publication types: Journal Article;Review
ElevatedBICD2DNA methylation in blood of major depressive disorder patients and reduction of depressive-like behaviors in hippocampalBicd2-knockdown mice.

Major depressive disorder (mdd) is a prevalent and devastating mental illness.To date, the diagnosis of mdd is largely dependent on clinical interviews and questionnaires and still lacks a reliable biomarker.Dna methylation has a stable and reversible nature and is likely associated with the course and therapeutic efficacy of complex diseases, which may play an important role in the etiology of a disease.Here, we identified and validated a dna methylation biomarker for mdd from four independent cohorts of the chinese han population.First, we integrated the analysis of the dna methylation microarray (n= 80) and rna expression microarray data (n= 40) and identified bicd2as the top-ranked gene.In the replication phase, we employed the sequenom mass array method to confirm the dna hypermethylation change in a large sample size (n= 1,346) and used the methylation-sensitive restriction enzymes and a quantitative pcr approach (mse-q pcr) and q pcr method to confirm the correlation between dna hypermethylation and m rna down-regulation of bicd2(n= 60).The results were replicated in the peripheral blood of mice with depressive-like behaviors, while in the hippocampus of mice,bicd2showed dna hypomethylation and m rna/protein up-regulation.Hippocampal bicd2knockdown demonstrates antidepressant action in the chronic unpredictable mild stress (cums) mouse model of depression, which may be mediated by increased bdnf expression.Our study identified a potential dna methylation biomarker and investigated its functional implications, which could be exploited to improve the diagnosis and treatment of mdd.

Journal: Proceedings of the National Academy of Sciences of the United States of America (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Impact of COVID-19 on acute trauma and orthopaedic referrals and surgery in the UK during the first wave of the pandemic: a multicentre observational study from the COVid Emergency-Related Trauma and orthopaedics (COVERT) Collaborative.

This is the first british multicentre study observing the impact of the covid-19 pandemic on orthopaedic trauma with respect to referrals, operative caseload and mortality during its peak.Design a longitudinal, multicentre, retrospective, observational, cohort study was conducted during the peak 6 weeks of the first wave from 17 march 2020 compared with the same period in 2019.Setting hospitals from six major urban cities were recruited around the uk, including london.Participants a total of 4840 clinical encounters were initially recorded.4668 clinical encounters were analysed post-exclusion.Primary and secondary outcome measures primary outcomes included the number of acute trauma referrals and those undergoing operative intervention, mortality rates and the proportion of patients contracting covid-19.Secondary outcomes consisted of the mechanism of injury, type of operative intervention and proportion of aerosolising-generating anaesthesia used.Results during the covid-19 period, there was a 34% reduction in acute orthopaedic trauma referrals compared with 2019 (1792 down to 1183 referrals), and a 29.5% reduction in surgical interventions (993 down to 700 operations).The mortality rate was more than doubled for both risk and odds ratios during the covid-19 period for all referrals (1.3% vs 3.8%, p=0.0005) and for those undergoing operative intervention (2.2% vs 4.9%, p=0.004).Moreover, mortality due to covid-19-related complications (vs non-covid-19 causes) had greater odds by a factor of at least 20 times.For the operative cohort during covid-19, there was an increase in odds of aerosolising-generating anaesthesia (including those with superimposed regional blocks) by three-quarters, as well as doubled odds of a consultant acting as the primary surgeon.Conclusion although there was a reduction of acute trauma referrals and those undergoing operative intervention, the mortality rate still more than doubled in odds during the peak of the pandemic compared with the same time interval 1 year ago.

Journal: BMJ open (2022)
Publication types: Journal Article;Multicenter Study;Observational Study
Cardiac crises: Cardiac arrhythmias and cardiomyopathy during TANGO2 deficiency related metabolic crises.

Tango2 deficiency disorder (tdd) is an autosomal recessive disease associated with metabolic crisis, lethal cardiac arrhythmias, and cardiomyopathy.Data regarding treatment, management, and outcomes of cardiac manifestations of tdd are lacking.Objective the purpose of this study was to describe tdd-related cardiac crises.Methods retrospective multicenter chart review was made of tdd patients admitted with cardiac crises, defined as development of ventricular tachycardia (vt), cardiomyopathy, or cardiac arrest during metabolic crises.Results twenty-seven children were admitted for 43 cardiac crises (median age 6.4 years; interquartile range [iqr] 2.4-9.8 years) at 14 centers.During crisis, qtc prolongation occurred in all (median 547 ms; iqr 504-600 ms) and a type i brugada pattern in 8 (26%).Arrhythmias included vt in 21 (78%), supraventricular tachycardia in 3 (11%), and heart block in 1 (4%).Nineteen patients (70%) developed cardiomyopathy, and 20 (74%) experienced a cardiac arrest.There were 10 deaths (37%), 6 related to arrhythmias.In 5 patients, recalcitrant vt occurred despite use of antiarrhythmic drugs.In 6 patients, arrhythmias were controlled after extracorporeal membrane oxygenation (ecmo) support; 5 of these patients survived.Among 10 patients who survived vt without ecmo, successful treatment included intravenous magnesium, isoproterenol, and atrial pacing in multiple cases and verapamil in 1 patient.Initiation of feeds seemed to decrease vt events.Conclusion tdd-related cardiac crises are associated with a high risk of arrhythmias, cardiomyopathy, cardiac arrest, and death.Although further studies are needed, early recognition and appropriate treatment are critical.Acutely, intravenous magnesium, isoproterenol, atrial pacing, and ecmo as a last resort seem to be the best current treatment options, and early initiation of feeds may prevent vt events.

Journal: Heart rhythm (2022)
Publication types: Journal Article;Multicenter Study;Research Support, Non-U.S. Gov't;Research Support, N.I.H., Extramural
Association between mortality and cardiovascular diseases in the vulnerable Mexican population: A cross-sectional retrospective study of the COVID-19 pandemic.

Cardiovascular diseases (cvds) continue to be the leading cause of death worldwide.Over the past couple of years and with the surge of the covid-19 pandemic, mortality from cvds has been slightly overshadowed by those due to covid-19, although it was during the peak of the pandemic.In the present study, patients with cvds (cvds;n= 41,883) were analyzed to determine which comorbidities had the largest impact on overall patient mortality due to their association with both diseases (n= 3,637).Obesity, hypertension, and diabetes worsen health in patients diagnosed positive for covid-19.Hence, they were included in the overview of all patients with cvd.Our findings showed that 1,697 deaths were attributable to diabetes (p< 0.001) and 987 deaths to obesity (p< 0.001).Lastly, 2,499 deaths were attributable to hypertension (p< 0.001).Using logistic regression modeling, we found that diabetes (or: 1.744,p< 0.001) and hypertension (or: 2.179,p< 0.001) significantly affected the mortality rate of patients.Hence, having a cvd diagnosis, with hypertension and/or diabetes, seems to increase the likelihood of complications, leading to death in patients diagnosed positive for covid-19.

Journal: Frontiers in public health (2022)
Publication types: Journal Article
The impact of the COVID-19 pandemic on elective laparoscopic cholecystectomy: A retrospective Cohort study.

The coronavirus disease 2019 (covid-19) pandemic had a significant impact on elective surgery for benign disease.We examined the effects of covid-19 related delays on the outcomes of patients undergoing elective laparoscopic cholecystectomy (lc) in an upper gastrointestinal surgery unit in the uk.We have analysed data retrospectively of patients undergoing elective lc between 01/03/2019 to 01/05/2019 and 01/04/2021 to 11/06/2021.Demographics, waiting time to surgery, intra-operative details and outcome data were compared between the two cohorts.Indications for surgery were grouped as inflammatory (acute cholecystitis, gallstone pancreatitis, cbd stone with cholangitis) or non-inflammatory (biliary colic, gallbladder polyps, cbd stone without cholangitis).Apvalue of <0.05 was used for statistical significance.Out of the 159 patients included, 106 were operated pre-pandemic and 53 during the pandemic recovery phase.Both groups had similar age, gender, asa-grades and bmi.In the pre-pandemic group, 68 (64.2%) were operated for a non-inflammatory pathology compared to 19 (35.8%) from the recovery phase cohort (p< 0.001).The waiting time to surgery was significantly higher amongst patients operated during the recovery phase (p= 0000.1).Less patients had complete cholecystectomy during the pandemic recovery phase (p= 0.04).There were no differences in intraoperative times and patient outcomes.These results demonstrate the impact of covid-19 related delays to our cohort, however due to the retrospective nature of this study, the current results need to be backed up by higher evidence in order for strong recommendations to be made.

Journal: Frontiers in surgery (2022)
Publication types: Journal Article
Catheter-Directed Interventions for the Treatment of Lower Extremity Deep Vein Thrombosis.

Lower extremity deep vein thrombosis (dvt) leads to significant morbidity including pain, swelling, and difficulty walking in the affected limb.If left untreated, dvt increases the risk of pulmonary embolism (pe), recurrent venous thromboembolism (vte), and post thrombotic syndrome (pts).The objective of this review was to identify catheter-directed interventions and their success rates for the treatment of lower extremity dvt.A comprehensive search of current and emerging catheter-directed interventions for lower extremity dvt treatment was conducted in pub med and google scholar.Clinical trials, retrospective and prospective observational studies, and case reports were identified to classify percutaneous mechanical thrombectomy (pmt), catheter-directed thrombolysis (cdt), and pharmacomechanical cdt (pcdt) devices based on their mechanism of action and indication of use.Catheter-directed interventions such as pmt, cdt, and pcdt offer an alternative therapeutic strategy for dvt management, particularly in patients with limb-threatening conditions and absolute contraindications to anticoagulants.Currently, there are limited guidelines for the use of mechanical and pharmacomechanical devices because of the lack of clinical evidence available for their use in treatment.Future studies are required to determine the short and long-term effects of using catheter-directed interventions as well as their effectiveness in treating acute versus subacute and chronic dvt.

Journal: Life (Basel, Switzerland) (2022)
Publication types: Journal Article;Review
Prevalence and mobility of integrative and conjugative elements within aStreptomycesnatural population.

Horizontal gene transfer (hgt) is a powerful force generating genomic diversity in bacterial populations.Hgt in streptomycesis in large part driven by conjugation thanks to plasmids, integrative and conjugative elements (ices) and actinomycete ices (aices).To investigate the impact of ice and aice conjugation on streptomycesgenome evolution, we usedin silicoand experimental approaches on a set of 11 very closely related strains isolated from a millimeter scale rhizosphere population.Through bioinformatic searches of canonical conjugation proteins, we showed that aices are the most frequent integrative conjugative elements, with the central chromosome region being a hotspot for integrative element insertion.Strains exhibited great variation in aice composition consistent with frequent hgt and/or gene loss.We found that single insertion sites can be home to different elements in different strains (accretion) and conversely, elements belonging to the same family can be found at different insertion sites.A wide variety of cargo genes was present in the aices with the potential to mediate strain-specific adaptation (e.g., dna metabolism and resistance genes to antibiotic and phages).However, a large proportion of aice cargo genes showed hallmarks of pseudogenization, consistent with deleterious effects of cargo genes on fitness.Pock assays enabled the direct visualization of conjugal aice transfer and demonstrated the transfer of aices between some, but not all, of the isolates.Multiple aices were shown to be able to transfer during a single mating event.Although we did not obtain experimental evidence for transfer of the sole chromosomal ice in this population, genotoxic stress mediated its excision from the chromosome, suggesting its functionality.Our results indicate that aice-mediated hgt in streptomycespopulations is highly dynamic, with likely impact on strain fitness and the ability to adapt to environmental change.

Journal: Frontiers in microbiology (2022)
Publication types: Journal Article
Accelerated Ultrafast Magnetization Dynamics at Graphene/CoGd Interfaces.

Atomically thin graphene layers can act as a spin-sink material when adjacent to a nanoscale magnetic surface.The enhancement in the extrinsic spin-orbit coupling (soc) strength of graphene plays an important role in absorbing the spin angular momentum injected from the magnetic surface after perturbation with an external stimulus.As a result, the dynamics of the excited spin system is modified within the magnetic layer.In this paper, we demonstrate the modulation of ultrafast magnetization dynamics at graphene/ferrimagnet interfaces using the time-resolved magneto-optical kerr effect (trmoke) technique.Magnetically modified interfaces with a systematic increase in the number of graphene layers coupled with the 10 nm-thick co74gd26layer are studied.We find that the variation in the dynamical parameters, i.e., ultrafast demagnetization time, remagnetization times, decay time, effective damping, precessional frequency, etc., observed at different time scales is interconnected.The demagnetization time and decay time for the ferrimagnet become approximately two times faster than the corresponding intrinsic values.We found a possible correlation between the demagnetization time and damping.The effect is more pronounced for the interfaces with monolayer graphene and graphite.The spin-mixing conductance is found to be approximately 0.8 × 1015cm-2.The effect of soc, pure spin current, the appearance of structural defects, and thermal properties at the graphene/ferrimagnet interface are responsible for the modifications of several dynamical parameters.This work demonstrates some important properties of the graphene/ferrimagnet interface which may unravel the possibilities of designing spintronic devices with elevated performance in the future.

Journal: ACS nano (2022)
Publication types: Journal Article
H2O2-responsive VEGF/NGF gene co-delivery nano-system achieves stable vascularization in ischemic hindlimbs.

Peripheral vascular disease (pvd) is a common clinical manifestation of atherosclerosis.Vascular endothelial growth factor (vegf) gene therapy is a promising approach for pvd treatment.However, due to single-gene therapy limitations and high h2o2pathological microenvironment, vegf gene therapy are not as expectations and its clinical application are limited.Synergistic effects of nerve factors and vascular factors in angiogenesis have attracted attention in recent years.In this study, vegf and nerve growth factor (ngf) genes co-delivery nanoparticles (vegf/ngf-nps) were prepared by using h2o2responsive 6s-plga-po-peg as a carrier.6s-plga-po-peg could react with h2o2specifically due to the internal peroxalate bond.Angiogenic effects of vegf/ngf-nps has been evaluated in cells and hindlimb ischemia mice model.Results showed that vegf/ngf-nps promoted vegf and ngf co-expression simultaneously, eliminated excessive h2o2, strengthened reactions between sh-sy5ys and huvecs, and finally enhanced migration, tube formation, proliferation and h2o2damage resistance of huvecs.Vegf/ngf-nps also recovered blood perfusion, promoted the expression of vegf, ngf, e nos and no, and enhanced vascular coverage of pericytes.Treatment effects of vegf/ngf-nps may related to vegf/e nos/no pathway.Altogether, vegf/ngf-nps eliminated excessive h2o2while achieving gene co-delivery, and promoted stable angiogenesis.It's a promising way for pvd treatment by using vegf/ngf-nps.

Journal: Journal of nanobiotechnology (2022)
Publication types: Journal Article
Fabrication and Optical Characterization of VO2-Based Thin Films Deposited on Practical Float Glass by Magnetron Sputtering and Professional Annealing.

In this paper, vo2thin films with good optical properties are fabricated on practical float glass by magnetron sputtering and a professional annealing method.The near-infrared switching efficiency (nirse) of the prepared film reaches 39% (@2000 nm), and its near-infrared energy modulation ability (δtir) reaches 10.9% (780-2500 nm).Further, the highest integral visible transmittance tlumis 63%.The proposed method exhibits good reproducibility and does not cause any heat damage to the magnetron sputtering machine.The crystalline structure of the vo2film is characterized by x-ray diffraction (xrd).The lattice planes (011) and (-211) grow preferentially (jcpds 65-2358), and a large number of na v2o5crystals are detected simultaneously.The microstructures are characterized by scanning electron microscopy (sem), and a large number of long sheet crystals are identified.The phase transition temperature is significantly reduced by an appropriate w doping concentration (tc= 29 °c), whereas excessive w doping causes distortion of the thermal hysteresis loop and a reduction in the nirse.Oxygen vacancies are created by low pressure annealing, due to which the phase transition temperature of vo2film decreases by 8 °c.The addition of an intermediate si o2layer can prevent the diffusion of na+ions and affect the preparation process of the vo2thin film.

Journal: Materials (Basel, Switzerland) (2022)
Publication types: Journal Article