syedzayyan/paper_mill_benchmark huggingface dataset:
Levosimendan Postconditioning Attenuates Cardiomyocyte Apoptosis after Myocardial Infarction

Levosimendan preconditioning has been shown to attenuate myocardial apoptosis in animal models.However, protective effects of levosimendan postconditioning against myocardial apoptosis following myocardial infarction (mi) have not been evaluated.Therefore, we investigated the effects of levosimendan postconditioning on myocardial apoptosis in mi rat models.Methods.In an anoxia/reoxygenation (a/r) model, h9c2 cells were pretreated with or without levosimendan postconditioning after which their apoptosis rates were assessed by flow cytometry, rt-q pcr, and western blot analyses.Then, postconditioning was performed with or without levosimendan in mi rat models.Myocardiocyte apoptosis was evaluated by echocardiography, ttc staining, tunel staining, immunohistochemical staining, rt-q pcr, and western blot analysis.Results.Levosimendan postconditioning inhibited h9c2 cell apoptosis in a/r models by elevating bcl-2 while suppressing caspase-3 and bax at both m rna and protein levels.Moreover, it improved cardiac functions and reduced the left ventricle infarction area in mi rat models.Compared to the mi control group, cardiomyocyte apoptosis rates in the levosimendan postconditioning group were low.The reduced cardiomyocyte apoptosis rates were associated with downregulation of bax and caspase-3 as well as with upregulation of bcl-2 at m rna and protein levels.Conclusions.Levosimendan postconditioning of mi rat models protected against cardiomyocyte apoptosis, implying that it is a potential strategy for preventing cardiomyocyte apoptosis in the treatment of cardiac dysfunction following mi.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Targeting AraC-Resistant Acute Myeloid Leukemia by Dual Inhibition of CDK9 and Bcl-2: A Systematic Review and Meta-Analysis

This study aims to determine the influence of targeting ara c-resistant acute myeloid leukemia by dual inhibition cyclin-dependent protein kinase (cdk9) and b-cell lymphoma-2 (bcl-2).Method.The c-myc inhibitor 10058-f4 and the cdk9 inhibitor azd4573 were used to determine the cell cycle arrest and apoptosis.Results.10058-f4 reduces c-myc protein levels and suppresses hep g2 cell proliferation, possibly by upregulating cyclin-dependent kinase (cdk) inhibitors, p21waf1, and reducing intracellular alpha-fetal protein (afp) levels.Conclusion.The combination of azd4573 and 10058-f4 has a synergistic anti-ara c-resistant aml activity, providing a solid database for the aforementioned scientific hypothesis.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Meta-Analysis;Review Article;
Intervention Effect of Traditional Chinese Medicine Hot Pressing Combined with Health Education on the Adolescent’s Visual Fatigue

To explore the intervention effect of traditional chinese medicine hot pressing combined with health education in adolescents with asthenopia, 92 adolescents with asthenopia admitted to the outpatient department of guangming traditional chinese medicine hospital in pudong new area from october 2019 to january 2021 were selected and randomly divided into two groups: the control group and the test group, each with 46 cases.Both received health education.The control group was given sodium hyaluronate eye drops, and the test group was given traditional chinese medicine hot ironing technique intervention.After 2 courses of treatment, the scores of visual fatigue symptoms, clinical curative effect, and eye refractive power of the two groups were observed.The satisfaction of treatment was compared between the two groups.The scores of asthenopia of the two groups were compared at 6 months after intervention.After the intervention, the scores of visual fatigue symptoms in the control group and the test group were reduced after one or two courses of treatment (control group: t = 4.167, 6.318, and p = 0.027 , 0.010; test group: t = 4.820, 6.834, and p = 0.013 , &lt;0.001).The scores of asthenopia symptoms of the trial group after the intervention for one and two courses were significantly lower than those of the control group p &lt; 0.05 ; the total clinical effective rate of the trial group was 93.48%, which was significantly higher than that of the control group (80.43%).The difference between the groups was statistically significant p &lt; 0.05 ; the left and right eyes of the control group did not change significantly before and after the intervention p &gt; 0.05 .After the intervention, the left and right eyes of the paper group were significantly reduced p &lt; 0.05 .After the intervention, the difference of the two groups in the refractive power of the left and right eyes was statistically significant p &lt; 0.05 .After treatment, the satisfaction of the experimental group was significantly higher than that of the control group p &lt; 0.05 .After 6 months, there was no significant change in the visual fatigue score of the experimental group, while the visual fatigue score of the control group increased significantly.The traditional chinese medicine ironing combined with health education intervention can improve the symptoms of adolescents’ asthenopia and improve the treatment efficiency.The method is safe, and the operation is convenient.It is worthy of clinical promotion.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
lncRNA MSC-AS1 Aggravates Diabetic Nephropathy by Regulating the miR-325/CCNG1 Axis

Diabetic nephropathy (dn) is the most common microvascular complication of diabetes and has become the second leading cause of end-stage renal disease in the world.This study aims to clarify the regulatory mechanism of the lnc rna msc-as1/mi r-325/cyclin g1 (ccng1) axis in dn.Methods.The regulatory mechanism of lnc rna msc-as1/mi r-325/ccng1 was evaluated by rt-q pcr, cck-8 assay, flow cytometry assay, rna pull-down assay, elisa, and western blot assay.Results.Upregulation of lnc rna msc-as1 was detected in dn patients and hrmc cells treated with high glucose (hg).Knockdown of lnc rna msc-as1 reduced the proliferation, fibrosis, and inflammation of hrmc cells induced by hg.In addition, lnc rna msc-as1 acts as a mi r-325 sponge in the dn.Ccng1 is the direct target of mi r-325, which can be positively regulated by lnc rna msc-as1 in dn.More importantly, downregulation of mi r-325 and upregulation of ccng1 can attenuate the protective effect of lnc rna msc-as1 knockdown on dn.Conclusion.Lnc rna msc-as1 aggravates dn by downregulating mi r-325 and upregulating ccng1.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
miR-146a Inhibited Pancreatic Cancer Cell Proliferation by Targeting SOX7

Micro rnas (mi rnas) act as a kind of small and noncoding rna, which have been implicated in the regulation of various pathobiological processes in cancer, including progression in pancreatic cancer and in other human cancers.Previous reports demonstrate that pancreatic cancer has been reported as one of the leading causes of cancer-related death, and some factors including oncogenic genes and environments are involved in tumorigenesis.In our study, we found micro rna-146a (mi r-146a) was evidently downregulated in pancreatic cancer tissues and cells.Overexpression of mi r-146a obviously reduced cell proliferation and tumorigenesis in vitro, as determined by mtt analysis, colony formation analysis, ed u analysis, and cell cycle experiments.Here, we found tumor suppressor sex-determining region y-box 7 (sox7) was the direct target of mi r-146a.Overexpression of mi r-146a decidedly inhibited sox7 expression, which promotes cell proliferation and tumorigenesis.Knockdown of mi r-146a increased sox7 expression.Depression of mi r-146a and sox7 promoted cell proliferation and tumorigenesis in vitro, confirming mi r-146a regulated pancreatic cancer cell proliferation by inhibiting sox7.In summary, we found mi r-146a reduced the cell proliferation of pancreatic cancer through targeting sox7.In the present study, we demonstrated the function of mi r-146a in pancreatic cancer and might provide a new target in the treatment of pancreatic cancer.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Construction and Validation of a Risk Prediction Model for Postoperative Urinary Retention in Lung Cancer Patients

Indwelling catheter is a routine procedure in surgical patients.Studies have shown that prolonged indwelling urinary catheterization increases the risk of postoperative urinary tract infection.Although early removal of the urinary catheter after operation can reduce the risk of postoperative urinary symptoms and tract infections, it may lead to postoperative anesthetic dysuria.Therefore, this study investigates the urinary retention and related risk factors in patients after thoracoscopic lobectomy under general anesthesia.The clinical data of 214 patients who underwent thoracoscopic lobectomy in the department of thoracic surgery of a tertiary class a cancer hospital in beijing from july 2020 to april 2021 were collected.A risk prediction model was established by logistic regression analysis, and the prediction effect was determined using the area under the receiver operating characteristic (roc) curve.The incidence of indwelling catheter after thoracoscopic lobectomy was 44.8% (96/214).Sex (or = 21.102, 95% ci: 2.906–153.239, p = 0.003 ), perception of shame (or = 74.256, 95% ci: 6.171–893.475, p = 0.001 ), age (or = 1.095, 95% ci: 1.014–1.182, p = 0.021 ), and bed rest time (or = 1.598, 95% ci: 1.263–2.023, p &lt; 0.021 ) were the factors influencing urinary retention after thoracoscopic lobectomy.This model can effectively predict the occurrence of postoperative urinary retention in patients with lung cancer and help medical staff to intervene effectively before the onset of urinary retention, which provides reference for preventive treatment and nursing intervention.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
LncRNA SBF2-AS1 Facilitates Nonsmall Cell Lung Cancer Progression by Targeting miR-520a-3p

Long noncoding rna (lnc rna) set-binding factor 2 (sbf2) antisense rna1 (sbf2-as1), which acts as an oncogene in various cancers, can promote tumors progression.The study aimed to explore the role and molecular mechanism of sbf2-as1 in nonsmall cell lung cancer (nsclc).Methods.Q rt-pcr was introduced to detect sbf2-as1 and mi r-520a-3p expression in nsclc.The effects of sbf2-as1 and mi r-520a-3p on the proliferation, migration, and invasion of nsclc cells were assessed through cell counting kit-8 (cck-8) and transwell assay.Furthermore, the relationship of sbf2-as1 and mi r-520a-3p was verified by the rna immunoprecipitation (rip) assay, dual-luciferase assay, and spearman correlation analysis.Results.In nsclc tissues, sbf2-as1 was highly expressed, while mi r-520a-3p expression has decreased.The overall survival of nsclc patients with high sbf2-as1 expression was lower.Sbf2-as1 silencing repressed the proliferation, migration, and invasion of nsclc cells.Sbf2-as1 directly interacted with mi r-520a-3p, and a negative relationship was observed between their expression levels in nsclc tissues.More importantly, the suppression of sbf2-as1 silencing on the proliferation, migration, and invasion in nsclc cells was counteracted by mi r-520a-3p inhibition.Conclusion.Sbf2-as1 accelerated the proliferation, migration, and invasion of nsclc cells via mediating mi r-520a-3p, thus promoting nsclc progression.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Evaluation of Tresiba Combined with Six Ingredient Rehmannia Pill in the Treatment of Type 2 Diabetes

Insulin replacement therapy is the main treatment method for type 1 diabetes, and adjuvant comprehensive treatment to reduce the complications of diabetes is still the focus of research.The purpose of this study is to explore the clinical efficacy of tresiba combined with ingredient rehmannia pill in the treatment of type 1 diabetes.Methods.A total of 216 patients with type 2 diabetes admitted to our hospital from january 2019 to july 2019 were enrolled in this study.Patients in the control and observation groups were treated with tresiba and tresiba combined with ingredient rehmannia pill, respectively.The change of tcm symptom score, blood glucose level and fasting insulin level before and after treatment were evaluated, and the insulin resistance index was calculated to observe the adverse reactions of patients.Results.After treatment, the tcm syndrome scores of the two groups decreased significantly, and the tcm syndrome scores of the observation group were significantly lower than those of the control group.The fasting blood glucose, 2 h postprandial blood glucose and insulin resistance index of the observation group were lower than those of the control group.The levels of fbg, 2 h bg and hb a1c in the observation group were significantly lower than those in the control group.The total effective rate of the observation group was 91.7%, which was significantly higher than that of the control group (77.1%).The adverse reactions of patients in the observation group were slightly more than those in the control group.Conclusion.Our study demonstrated that ingredient rehmannia pill combined with tresiba is effective in the treatment of type 2 diabetes, providing alternative therapies for the treatment of diabetes.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
The Effects of Yoga Exercise on Pelvic Floor Rehabilitation of Postpartum Women

Rehabilitation of the pelvic floor after delivery is very important for women.Pelvic floor rehabilitation can speed up the recovery of the postpartum vagina and pelvic floor muscle tension and elasticity and have a good effect on the prevention and treatment of postpartum vaginal prolapse and relaxation, urinary incontinence and other pelvic floor disorders.Thus, this article focuses on yoga exercise to explore its impact on postpartum pelvic floor rehabilitation.This article uses electrical stimulation and the treatment of pelvic floor muscles combined with the posture recognition algorithm, the yoga rehabilitation training program that has the best effect on the parturient is obtained, and the yoga myoelectric stimulation combined method and the traditional myoelectric stimulation method are designed for comparison experiments.The experimental results show that the parturients who have undergone the combined method of yoga myoelectric stimulation, in the resting state, contraction state, and valsalva state, the position of the bladder meridian, the position of the uterus, and the position of the rectal ampulla of the parturient have a significant recovery compared those who have undergone the traditional electromyography treatment.In addition, the average area of hiatus in the pelvic floor ultrasound examination in the control group 42 days postpartum was 12.2605 cm2, while the average area of the hiatus in the pelvic floor ultrasound examination in the experimental group 42 days postpartum was 10.788 cm2; the average area of hiatus in the pelvic floor ultrasound examination in the control group at 3 months postpartum was 11.4805 cm2, and the average area of hiatus in the pelvic floor ultrasound examination in the experimental group at 3 months postpartum was 8.9475 cm2.To sum up, yoga had a very significant improvement on the physical indicators and mental health of postpartum women.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Effects of Modified Wenjing Decoction Combined with Online Publicity and Education on the Treatment of Primary Dysmenorrhea of Cold Coagulation and Blood Stasis

To explore the effects of modified wenjing decoction combined with online publicity and education on the treatment of primary dysmenorrhea of cold coagulation and blood stasis.Methods.The materials of 111 patients with primary dysmenorrhea of cold coagulation and blood stasis in the outpatient department (january 2019–june 2021) were collected to conduct the retrospective study.The 111 patients were randomized into treatment group (n = 59) and control group (n = 52).The control group received online publicity and education and conventional treatment, and the treatment group received online publicity and education and modified wenjing decoction.The patients in the two groups were continuously treated for three menstrual cycles.The treatment effects, the dosage of analgesics, the scores of associated symptoms before and after treatment, and other indexes were compared between the two groups.Results.The differences in the efficacy on abdominal pain were statistically significant between the two groups p &lt; 0.05 .Compared with the control group, the treatment group had lower scores of associated symptoms after treatment p &lt; 0.5 .After the treatment of three menstrual cycles, 54 patients in the treatment group stopped taking ibuprofen, and the average ibuprofen dosage of the other 5 patients was (0.24 ± 0.16)g.The 52 patients in the control group still needed to take ibuprofen, and the mean dosage was (0.51 ± 0.05)g.The differences in the ibuprofen dosage between the two groups had remarkable difference p &lt; 0.001 .Conclusion.Modified wenjing decoction combined with online publicity and education can obviously improve the clinical symptoms of the patients with primary dysmenorrhea of cold coagulation and blood stasis and reduce the dosage of analgesics.It is worth promoting and applying in practice.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Bioinformatic Analysis of PTTG Family and Prognosis and Immune Cell Infiltration in Gastric Cancer

Gastric cancer is the sixth highest incidence rate in the world.Although treatment has made progress, the prospect of gastric cancer patients is bleak.Difficulties and future prospects of immunotherapy in cancer treatment.Adaptive cell therapy, cancer vaccines, gene therapy, and monoclonal antibody therapy have all been used in gastric cancer with some initial success.Pttgs (pituitary tumor-transforming genes) have been proven to be closely related to the prognosis of many malignant tumors.However, the prognosis and immune cell infiltration of gastric adenocarcinoma (stad) remain unclear.We retrieved multiple databases to understand the possible activity of pttgs and their expression in gastric cancer, as well as their relationship with clinical data, overall survival rate, first progression, and survival rate after progression.Pttgs are overexpressed in stad tumor tissues.Many clinical variables are closely related to pttgs.In addition, pttg was associated with overall survival independent of disease.In addition, the expression of pttg1/2 was positively correlated with the molecular status of the immune checkpoint and negatively correlated with the infiltration of various immune cells.Data research shows that pttg and stad are closely related.This paved the way for future research, revealed the complex pathophysiology of gastric cancer, and introduced an effective new treatment.

Journal: Stem Cells International (2023)
Publication types: Research Article;
Advanced Cognitive Algorithm for Biomedical Data Processing: COVID-19 Pattern Recognition as a Case Study

Automated disease prediction has now become a key concern in medical research due to exponential population growth.The automated disease identification framework aids physicians in diagnosing disease, which delivers accurate disease prediction that provides rapid outcomes and decreases the mortality rate.The spread of coronavirus disease 2019 (covid-19) has a significant effect on public health and the everyday lives of individuals currently residing in more than 100 nations.Despite effective attempts to reach an appropriate trend to forecast covid-19, the origin and mutation of the virus is a crucial obstacle in the diagnosis of the detected cases.Even so, the development of a model to forecast covid-19 from chest x-ray (cxr) and computerized tomography (ct) images with the correct decision is critical to assist with intelligent detection.In this paper, a proposed hybrid model of the artificial neural network (ann) with parameters optimization by the butterfly optimization algorithm has been introduced.The proposed model was compared with the pretrained alex net, goog le net, and the svm to identify the publicly accessible covid-19 chest x-ray and ct images.There were six datasets for the examinations: three datasets with x-ray pictures and three with ct images.The experimental results approved the superiority of the proposed model for cognitive covid-19 pattern recognition with average accuracy 90.48, 81.09, 86.76, and 84.97% for the proposed model, support vector machine (svm), alex net, and goog le net, respectively.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Construction and Validation of a CNV-Driven Ferroptosis-Related Gene Signature for Predicting the Prognosis of Lung Adenocarcinoma

Previous studies have shown that ferroptosis plays an integral role in the development of cancer and copy number variations (cnvs) have been reported to associated with the ferroptosis.However, the role of cnvs-driven ferroptosis-related genes (frgs) in lung adenocarcinoma (luad) continues to be poorly understood.Therefore, we aimed to establish a novel gene signature in luad based on cnvs-driven ferroptosis-related genes.Methods.The transcriptome data and clinical features of luad patients were downloaded from the gene expression omnibus (geo) database and the cancer genome atlas (tcga) database.Differential analysis was carried out to recognize differentially expressed cnv-driven frgs.Univariate cox and least absolute shrinkage and selection operator (lasso) regression analyses were utilized to identify prognosis-associated genes.Kaplan-meier (k-m) analysis was a builder to estimate the worth of model.In addition, the nomogram was created to estimate survival probability of each patient.Ultimately, the immune microenvironment landscape between high and low risk groups was evaluated.Results.A total of 22 differentially expressed cnv-driven frgs were acquired in luad.These genes were significantly associated with serine family amino acid metabolism, iron regulation, reactive oxygen species metabolism, and cellular response to oxidative stress, and were involved in amino acid metabolism, malaria, amino acid biosynthesis, and hif-1 signaling pathways.Moreover, on the strength of 6 genes (tfap2a, slc2a1, aurka, cdo1, slc7a11, and alox5), the prognostic model was created, and the luad samples were significantly fall into the high- and low-risk groups, with the high-risk group had a poorer prognosis.Furthermore, risk score was an independent prognostic element.The nomogram with excellent predictive performance was developed for calculating the final result of luad patients at 1, 2, and 3 years.Finally, 19 immune cells had different infiltration differences among groups.Conclusion.A novel cnv-driven ferroptosis-related prognosis was established and could be used as a predictive indicator in luad.However, further clinical and in vivo in vitro experiments are necessary.

Journal: Journal of Sensors (2022)
Publication types: Research Article;