syedzayyan/paper_mill_benchmark huggingface dataset:
miR-196a-2 Promotes Malignant Progression of Thyroid Carcinoma by Targeting NRXN1

Thyroid cancer (tc) is the most common endocrine malignant disease with a rising morbidity year by year.Accumulating studies have shown that micro rnas (mi rnas) play a regulatory role in the progression of various tumors, but the molecular regulatory mechanism of mi r-196a-2 in tc is still unknown.Q rt-pcr was employed to measure the expression of mi r-196a-2 and nrxn1 m rna in tc cells, while western blot was used to detect the protein expression of nrxn1.Cck-8, colony formation and flow cytometry assays were used to measure cell proliferation and apoptosis of tc cells.Dual-luciferase reporter gene assay was used to predict and verify the targeted binding relationship between mi r-196a-2 and nrxn1.Our study results manifested that mi r-196a-2 was dramatically overexpressed in cells of tc, while nrxn1 was lowly expressed.Mi r-196a-2 could promote cell proliferation and inhibit cell apoptosis of tc.Additionally, mi r-196a-2 could also target and inhibit the expression of nrxn1.Silencing nrxn1 could reverse the inhibitory effect of mi r-196a-2 downregulation on cell proliferation of tc, as well as the promoting effect on cell apoptosis.In a conclusion, we found that mi r-196a-2 could promote cell proliferation and inhibit cell apoptosis of tc by targeting nrxn1.Therefore, mi r-196a-2/nrxn1 is potential to be a molecular therapeutic target for tc.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Research Article;
miR-19a-3p Promotes Tumor-Relevant Behaviors in Bladder Urothelial Carcinoma via Targeting THBS1

Mi r-19a-3p is widely increased in several cancers and can be used as an oncogenic factor in these cancers.However, the molecular mechanism of mi r-19a-3p in bladder urothelial carcinoma (blca) is still open.So, the study was aimed at exploring the mechanism of mi r-19a-3p in blca cells.Methods.Bioinformatics analysis was employed to find the differential mi rnas and m rnas, and the target mi rna and m rna were determined.Real-time quantitative pcr was used to evaluate mi r-19a-3p and thbs1 levels in human urethral epithelial cells and blca cells.Western blot was carried out to assay protein expression of thbs1 in human urethral epithelial cells and blca cells.Behaviors of blca cells were detected through cellular functional assays.Dual-luciferase gene assay was conducted to validate the binding of mi r-19a-3p and thbs1.Results.Mi r-19a-3p was increased in blca cells, while thbs1 was less expressed in blca cells.The mi r-19a-3p functions as an oncogene in blca.Thbs1 was a target of mi r-19a-3p, and it could reverse the promotion of mi r-19a-3p on cell malignant behaviors in blca.Conclusion.Mi r-19a-3p facilitates cell progression in blca via binding thbs1, which may be an underlying therapeutic target for blca treatment.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Research Article;
miR-204-5p Hampers Breast Cancer Malignancy and Affects the Cell Cycle by Targeting PRR11

To unravel mechanisms of mi r-204-5p in breast cancer (bc) cells.Methods.Mi r-204-5p expression level in bc cell lines was measured by q rt-pcr.Putative binding sites of mi r-204-5p on the 3 ′ -untranslated region of prr11 were predicted by the bioinformatics method and verified by the dual-luciferase method.Protein and m rna levels of prr11 in bc were determined by western blot and q rt-pcr.The association between two genes was analyzed by correlation analysis.Cancer cell functions were evaluated through cck8, flow cytometry, and transwell approaches.Results.Significant downregulation of mi r-204-5p was observed in bc tissue and cells.Cell functional experiments showed the inhibition of mi r-204-5p on cell behaviors and cell cycle.Prr11 was the downstream target of mi r-204-5p.Inhibition of rpp11 could reverse the impacts of the mi r-204-5p inhibitor on cell functions of bc.Conclusion.Our study revealed that the mi r-204-5p/prpp11 axis suppressed bc progression, which may provide a novel insight into the regulatory roles of mi r-204-5p.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
miR-223-3p Regulates NLRP3 to Inhibit Proliferation and Promote Apoptosis of ONG Cells

Optic nerve glioma (ong) is a rare disease, defined as a who grade i tumor, which affects the visual pathway.The objective of this study was to investigate the expression of mi r-223-3p in ong as well as its function and regulation in ong cell lines.Methods.Q rt-pcr assays were used to measure mi r-223-3p expression in ong tissues and cell lines.After overexpression of mi r-223-3p in hs683 and weri-rb-1 cell lines, cck-8 and ed u assays were performed to examine cell proliferation, and flow cytometry was used to assess apoptosis.Dual luciferase assays were utilized to identify the target binding to mi r-223-3p and nlrp3.Rescue assays were carried out to investigate the regulatory mechanism of mi r-223-3p acting through nlrp3.Nude mouse tumorigenesis assays were established to verify the effect of mi r-223-3p on ong growth.Results.Mi r-223-3p was weakly expressed in both ong tissues and cell lines.Mi r-223-3p inhibited the proliferative ability of hs683 and weri-rb-1 cell lines and promoted apoptosis.In addition, there was binding between mi r-223-3p and nlrp3.Simultaneous overexpression of nlrp3 and mi r-223-3p partially counteracted the role of mi r-223-3p in the cell lines.Lastly, mi r-223-3p inhibited ong growth.Conclusion.Mi r-223-3p plays an inhibitory role in ong development by regulating nlrp3 to inhibit the proliferation of ong cells and promote apoptosis.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
miR-381-3p Involves in Glioma Progression by Suppressing Tumor-Promoter Factor ANTXR1

Accumulating studies revealed association between development of glioma and mi rna dysregulation.A case in point is mi r-381-3p, but its mechanism in glioma is unclear yet.In this work, we confirmed that overexpressed mi r-381-3p repressed biological functions of glioma cells.Additionally, we also discovered that upregulated anthrax toxin receptor 1 (antxr1) was negatively mediated by mi r-381-3p.We further proved that mi r-381-3p-targeted antxr1 was able to counteract the suppression of mi r-381-3p on biological functions of glioma.We concluded that mi r-381-3p and antxr1 were both important factors in modulating glioma progression.Mi r-381-3p/antxr1 axis is expected to be a molecular target for glioma.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Research Article;
MiroRNA-31-3p Promotes the Invasion and Metastasis of Non-Small-Cell Lung Cancer Cells by Targeting Forkhead Box 1 (FOXO1)

To explore the possibility of micro rna mi r-31-3p as a biomarker for bone metastasis of non-small-cell lung cancer (nsclc) and its molecular mechanism to the invasion and metastasis of nsclc cells.Methods.Real-time quantitative pcr (rt-q pcr) was used to detect the expression levels of mi r-31-3p and forkhead box 1 (foxo1) in nsclc tissues, serum, and cells to analyze the correlation between the expression levels of mi r-31-3p and the clinicopathology of nsclc.After interference with or overexpressing mi r-31-3p, nsclc cell proliferation, apoptosis, invasion ability, and migration ability were detected by mtt, flow cytometry, transwell, and scratch experiment, respectively.The interaction between mi r-31-3p and foxo1 was further verified by the dual-luciferase reporter experiment.Western blot was performed to detect the protein expression of foxo1 in tissues and foxo1, rho a, p-rho a, rock-2, and p-rock-2 in cells.Results.In tissues, serum, and nsclc cell line a549 of the nsclc patients, the expression of foxo1 was notably lower, and the mi r-31-3p expression was significantly higher.Overexpression of mi r-31-3p could distinctly improve the proliferation, invasion, and migration of a549 cells, meanwhile inhibit cell apoptosis, and activate the rho a/rock-2 signaling pathway, while interfering with the expression of mi r-31-3p has the opposite function.Besides, bioinformatics analysis and luciferase reporter assay confirmed that foxo1 was a target gene of mi r-31-3p.Overexpressing foxo1 could inhibit the proliferation and metastasis of a549 cells, but overexpressing mi r-31-3p reverses the results.Conclusion.This study confirmed that mi r-31-3p promotes the proliferation, invasion, and migration of nsclc cells and inhibits apoptosis through targeted regulating foxo1 and be a potential therapeutic targets for the treatment of nsclc.

Journal: Computational and Mathematical Methods in Medicine (2022)
Publication types: Research Article;
miR-18a-5p Facilitates Malignant Progression of Head and Neck Squamous Cell Carcinoma Cells via Modulating SORBS2

This study attempted to investigate possible molecular mechanism and role of mi r-18a-5p in head and neck squamous cell carcinoma (hnscc).Differential mi rnas and their possible targets were analyzed through tcga database.By conducting q rt-pcr, mi r-18a-5p was tested to be increased and sorbs2 was assessed to be downregulated in hnscc cells.Cck-8, transwell, and flow cytometry assays disclosed that mi r-18a-5p facilitated hnscc cell proliferation, migration, and invasion and repressed cell apoptosis.By dual-luciferase reporter gene assay, it was verified that mi r-18a-5p had binding sites into sorbs2.Rescue experiments displayed that forced expression of sorbs2 restored the impact of mi r-18a-5p overexpression on hnscc cells.Collectively, our research preliminarily identified the promotion effect of mi r-18a-5p/sorbs2 axis on malignant phenotypes of hnscc cells.Our findings may provide a preclinical reference for hnscc treatment.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Research Article;
Pharmaceutical Reagent Inventory Strategy Based on Contract Shelf Life and Patient Demand

As the function and r&amp;d level of in vitro diagnostic reagents continue to improve, the need for hospitals for in vitro diagnostic reagents in clinical diagnosis also keeps increasing.However, under the influence of management, process, technology, equipment, materials, employees, and other unexpected disturbing factors, the output of reagents often has random uncertainty, and it is difficult to provide the finished products required by orders on time, in quality and quantity.A secondary supply chain consisting of reagent manufacturers, distributors, and hospitals is constructed, and the inventory control models of in vitro diagnostic reagent supply chain under three strategies of centralized decision‐making, hospital‐owned inventory, and reagent distributor‐managed inventory are established, respectively, and the maximum expected returns of the supply chain system under different strategies are analyzed to achieve the optimal production decision of reagent manufacturers and the optimal procurement decision of hospitals.The results show that reducing the random output probability and patient demand uncertainty has a significant effect on improving the expected return of in vitro diagnostic reagent supply chain, and as the random output probability of reagent manufacturers and patient consumption demand uncertainty increase, the strategy of managing inventory by distributors in collaboration is always better than the strategy of managing inventory by hospitals' own warehouses, which can achieve higher expected return and better inventory quantity, but when the out‐of‐stock cost of hospitals is too high above a certain threshold, the hospital will tend to adopt the self‐inventory strategy.

Journal: Contrast Media & Molecular Imaging (2022)
Publication types: Research Article;
miR-138 Reduces the Dysfunction of T Follicular Helper Cells in Osteosarcoma via the PI3K/Akt/mTOR Pathway by Targeting PDK1

To study the effect of mi r-138 on the function of osteosarcoma (os) t follicular helper cells (tfh cells) and its mechanism.Methods.Peripheral blood mononuclear cells (pbmcs) were isolated from patients with osteosarcoma (os group) and healthy volunteers (control group).Cd4+cxcr5+ tfh cells and cd9+ b cells were sorted by flow cytometry.Q rt-pcr was used to detect the expression of mi r-138 and pdk1 in the peripheral blood and cd4+cxcr5+ tfh cells.Flow cytometry was employed to detect the proportion of cd4+cxcr5+ tfh cells in cd4+ t cells, the level of cd40l in cd4+cxcr5+ tfh cells, and the expression of cd27 and cd38 in b cells.Western blot was used to determine the protein expression of pdk1, pi3k, p-akt, akt, p-m tor, and m tor.In addition, dual-luciferase reporter assay was performed to verify the relationship between mi r-138 and pdk1.Elisa method was used to determine the levels of ig m, ig g, il-10, and il-21.Results.Compared with that of the control group, the expression of mi r-138 in pbmc and cd4+cxcr5+ tfh cells of the os group was lower; overexpression of mi r-138 could promote the maturation of tfh cells and immature b cells.The results of the dual-luciferase report experiment showed that mi r-138 can target and negatively regulate pdk1, and pdk1 can reverse the effect of mi r-138 on the function of tfh cells and immature b cells.Conclusion.Mi r-138 inhibits the pi3k/akt/m tor pathway by targeting and negatively regulating pdk1 to alleviate the dysfunction of t follicular helper cells in os.

Journal: Computational and Mathematical Methods in Medicine (2021)
Publication types: Clinical Study;
Research on the Inheritance of Miao Costume Culture Based on Digital Wireless Communication Technology

The protection of the popular culture of local ethnic minorities is an inevitable requirement for building a multicultural system.Through the latest digital technology, through the internet, digital images, and other technologies, traditional cultural resources are classified and integrated, so that they can be inherited.The purpose of this study is to study the cultural heritage of digitized miao costumes.This paper introduces the background and importance of the digital protection of miao costume culture, analyzes in detail the current situation of miao costume culture, the application of digital protection technology at home and abroad, and analyzes and organizes the research content that introduces the importance of digital protection of miao costumes.Provide real data support for clothing culture protection.Collect relevant data through questionnaire survey, conduct quantitative statistical analysis, and study the impact of modern cultural communication tools such as television, mobile phones, and the internet on the inheritance of miao costume culture under the digital background.The results showed that only 5 of the 216 people were reluctant to use digital dissemination of miao costumes.

Journal: Mobile Information Systems (2022)
Publication types: Research Article;
On the Problems and Countermeasures of College Students’ Mental Health and Safe Work under Network Environment

Under the network environment, mental health and safety work on college is related to national security and campus security.Moreover, it affects the healthy development of college students.In the era of ever‐changing mobile internet, colleges and universities must take practical countermeasures against the problems in mental health and safety education on college and improve the relevance and effectiveness of college students’ network safety education.This paper first finds the problems of college students’ mental health and safety through questionnaire survey and analyzes the current situation of college students’ mental health and safety under the current networked environment.In response to the problems found, we propose complete and systematic educational countermeasures.At the same time, we need to firmly establish the strategic awareness of network security education and strengthen the construction of network security education in colleges and universities.We should strengthen the construction of network security education in colleges and universities, form a joint effort of schools, families, government, and society, and improve the quality and effectiveness of network security education.

Journal: Journal of Environmental and Public Health (2022)
Publication types: Research Article;
Nursing Methods and Experience of Local Anesthesia Patients under Arthroscope

In order to solve the nursing problems of local anesthesia patients under arthroscopy, a nursing method and experience based on local anesthesia patients under arthroscopy was proposed.From june 2019 to may 2021, 478 patients who underwent knee arthroscopy under spinal anesthesia or local anesthesia were retrospectively investigated, including 186 cases (38.9%) under local anesthesia and 292 cases (61.1%) under spinal anesthesia.2% lidocaine plus epinephrine was injected locally and intra-articular in patients with local anesthesia, and 0.75% bupivacaine in patients with spinal anesthesia.It was found that in the local anesthesia group and spinal anesthesia group, 94.1% (175/186) and 98.3% (287/292) patients did not feel pain during operation.93.0% (173/186 cases) and 96.2% (281/292 cases) of patients in the two groups were satisfied or very satisfied with the effect of anesthesia, respectively.The experimental results showed that local anesthesia was a simple and effective anesthesia method for knee arthroscopy, which was more reliable and safer than spinal anesthesia.Local anesthesia could be used for knee arthroscopy or cleaning and rinsing, free body removal, or even common meniscinoplasty.

Journal: Scanning (2022)
Publication types: Research Article;
Research on Pattern Recognition of Lower Limb Motion Based on Convolutional Neural Network

Accurate motion recognition is essential for assist devices such as exoskeletons to achieve human-robot communion.However, at present, the technology of lower limb motion pattern recognition still has the problems of small amount of data and low recognition accuracy.In this paper, the lower limb motion was taken as the object, and the surface electromyography (s emg) signals of five gaits of going upstairs without weight, going downstairs without weight, going upstairs with weight, going downstairs with weight, and walking on a level surface without weight were collected.Based on the feature extraction of the s emg signal, a convolutional neural network (cnn) with a feature set as the input is constructed, and a new lower limb motion pattern recognition method is proposed.The recognition accuracy and work feature of the proposed method are compared with several other classification and recognition methods.The experimental results show that, compared with the traditional methods, the method of using the feature set as input for cnn can better represent the features of the prediction model, and the pattern recognition accuracy is higher.The recognition accuracy of the five gaits are all greater than 96.96%, and the error rate is less than 7%, indicating that the proposed method has higher recognition accuracy.This method provides theoretical support for achieving compliant power assistance and promoting motor function rehabilitation with rehabilitation robots, power-assisted robots, and other equipment.

Journal: Wireless Communications and Mobile Computing (2022)
Publication types: Research Article;
Working Condition Monitoring System of Substation Robot Based on Video Monitoring

In order to realize intelligent video surveillance of substation, this paper presents a substation robot working state monitoring system based on video surveillance.In this paper, the intelligent monitoring system of substation robot working state is constructed by combining virtual reality technology.Through the cooperation of each unit in the system, the real-time monitoring of substation robot working state and the early warning of abnormal working state are realized.The system can transmit video data at a high frame rate to ensure the timeliness of video data transmission and clearly and smoothly present the virtual scene of substation robot work.It can realize the high-precision positioning measurement of the robot under different distances and speeds, obtain the working dynamic trajectory of the robot according to the measurement results, analyze its working state, and give an early warning in case of abnormal working state, so as to achieve the purpose of intelligently monitoring the working state of the substation robot.The experimental results show that each group of video data transmission frame rate of the system can reach 29 f/s and is relatively stable.The average transmission frame rate is 30.62 f/s.The video transmission frame rate is high, which can ensure the timeliness of video transmission.Conclusion.The designed substation intelligent video monitoring system has good output stability and reliability.

Journal: Wireless Communications and Mobile Computing (2022)
Publication types: Research Article;
miR-139-5p Inhibits Lung Adenocarcinoma Cell Proliferation, Migration, and Invasion by Targeting MAD2L1

Mi r-139-5p is lowly expressed in various human cancers and exerts its antitumor effect through different molecular mechanisms, yet the molecular mechanism of mi r-139-5p in lung adenocarcinoma (luad) remains to be further elucidated.The study is aimed at investigating the role and the regulatory mechanism of mi r-139-5p in luad progression.Methods.Differential analysis was performed on mi rna expression data in the tcga-luad dataset.Q rt-pcr was employed to detect the transcription levels of mi r-139-5p and mad2l1 in luad cells, while western blot was carried out for the detection of mad2l1 protein expression.Cck-8 and transwell assays were implemented to assess luad cell proliferation, migration, and invasion.A dual-luciferase reporter gene assay was conducted to verify the direct targeting relationship between mi r-139-5p and mad2l1.Results.Mi r-139-5p was significantly downregulated in luad cells in comparison with that in human normal bronchial epithelial cells.Overexpressing mi r-139-5p inhibited luad cell proliferation, migration, and invasion, while opposite results could be observed when mi r-139-5p was inhibited.Mad2l1 was identified as a direct target of mi r-139-5p in luad.Besides, the inhibitory effect of mi r-139-5p overexpression on luad cell proliferation, migration, and invasion was attenuated by overexpressing mad2l1.Conclusion.Our study suggests that mi r-139-5p is lowly expressed in luad cells and inhibits luad cell proliferation, migration, and invasion by targeted suppressing mad2l1 expression.It is of potential significance for the prognosis and treatment of luad.

Journal: Computational and Mathematical Methods in Medicine (2020)
Publication types: Research Article;