syedzayyan/paper_mill_benchmark huggingface dataset:
Review: Auxetic Polymer-Based Mechanical Metamaterials for Biomedical Applications.

Over the last three decades but more particularly during the last 5 years, auxetic mechanical metamaterials constructed from precisely architected polymer-based materials have attracted considerable attention due to their fascinating mechanical properties.These materials present a negative poisson's ratio and therefore unusual mechanical behavior, which has resulted in enhanced static modulus, energy adsorption, and shear resistance, as compared with the bulk properties of polymers.Novel advanced polymer processing and fabrication techniques, and in particular additive manufacturing, allow one to design complex and customizable polymer architectures that are particularly relevant to fabricate auxetic mechanical metamaterials.Although these metamaterials exhibit exotic mechanical properties with potential applications in several engineering fields, biomedical applications seem to be one of the most relevant with a growing number of articles published over recent years.As a result, special focus is needed to understand the potential of these structures and foster theoretical and experimental investigations on the potential benefits of the unusual mechanical properties of these materials on the way to high performance biomedical applications.The present review provides up to date information on the recent progress of polymer-based auxetic mechanical metamaterials mainly fabricated using additive manufacturing methods with a special focus toward biomedical applications including tissue engineering as well as medical devices including stents and sensors.

Journal: ACS biomaterials science & engineering (2022)
Publication types: Journal Article;Review;Research Support, Non-U.S. Gov't
Nursing care for patient in post operatory heart surgery in the Intensive Care Unit.

To investigate the critical nodes related to nursing care for patients in the postoperative period of cardiac surgery.Methods exploratory study with a qualitative approach.Data collected through semi-structured interviews with 27 members of the nursing team working in the intensive care unit.Material submitted to thematic analysis.Results three categories emerged: flaws in the professional qualification for patient care in the postoperative period of cardiac surgery; team challenges concerning specific patient care in the postoperative period of cardiac surgery; and (dis) organization of work in the intensive care unit and its impact on nursing care for patients in the postoperative period of cardiac surgery.Final considerations given the identification of the critical nodes, the professionals presented suggestions to overcome daily difficulties: investments in strategies for permanent education in health; creation of tools to guide patient assistance in the postoperative of cardiac surgery; and provision of adequate human resources.

Journal: Revista brasileira de enfermagem (2021)
Publication types: Journal Article
Circ-0005105 activates COL11A1 by targeting miR-20a-3p to promote pancreatic ductal adenocarcinoma progression.

Growing evidence indicates that circular rnas (circ rnas) are closely involved in tumorigenesis, but the association between circ rnas and pancreatic ductal adenocarcinoma (pdac) is far from clear.Here, we focused on the functional investigation of circ-0005105, a newly identified circ rna, in pdac progression.In the present study, we assessed circ-0005105 expression in pdac tissues and cell lines with quantitative reverse transcription-polymerase chain reaction (q rt-pcr).The biological functions of circ-0005105 in cellular proliferation and invasion were identified through gain- and loss-of-function experiments in vitro and in vivo.The interaction between circ-0005105 and the micro rna (mi r)-20a-3p-col11a1 (collagen type xi alpha 1) axis was examined using luciferase reporter and rna immunoprecipitation assays.We found that circ-0005105 expression was upregulated in both pdac tissues and cell lines.Higher circ-0005105 expression correlated positively with the malignant clinical phenotype and poor prognosis of patients with pdac.Gain- and loss-of-function analysis showed that circ-0005105 facilitated both in vitro and in vivo cellular proliferation and invasion.Mechanistically, circ-000510 served as a competing endogenous rna (ce rna) of mi r-20a-3p and indirectly modulated col11a1 expression, leading to activation of epithelial-mesenchymal transition (emt).Rescue experiments suggested that the oncogenic activity of circ-0005105 was dependent on the modulation of the mi r-20a-3p-col11a1 axis.More importantly, col11a1 overexpression was significantly associated with poor prognosis in pdac, and silencing col11a1 reduced pdac cell tumorigenicity and metastasis.Taken together, our findings confirm for the first time that circ-0005105 has critical functions by regulating the mi r-20a-3p-col11a1 axis.In the clinic, circ-0005105 can act as a potential prognostic marker and therapeutic target in pdac.

Journal: Cell death & disease (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
A Patient With Hemoglobin SC Disease and Acute Ischemic Stroke Presenting With Altered Mental Status.

A 57-year-old woman with a history of hypertension, diabetes mellitus, obesity, asthma, and hemoglobin sc disease presented to the emergency department by her home health aide after she was found having altered mental status.According to her home health aide, the patient was responding with "ok" to her questions for more than a day.The hemoglobin on admission was 8.5 g/d l.A magnetic resonance imaging (mri) without contrast of the brain showed acute cortical infarcts superimposed on the old infarct zone.The patient received 1 unit of packed red blood cells and a session of exchange transfusion, in addition to aspirin, clopidogrel, and atorvastatin during the hospital stay.When a patient known to have sickle cell disease presents with acute neurological deficits, the first consideration is usually acute ischemic stroke due to vaso-occlusion in the cerebral vessels.However, it is essential to not overlook other potential causes of acute neurological deficits.

Journal: Cureus (2021)
Publication types: Case Reports;Journal Article
Urinary l-erythro-β-hydroxyasparagine-a novel serine racemase inhibitor and substrate of the Zn2+-dependent d-serine dehydratase.

In the present study, we identified l-erythro-β-hydroxyasparagine (l-β-ehasn) found abundantly in human urine, as a novel substrate of zn2+-dependent d-serine dehydratase (dsd).L-β-ehasn is an atypical amino acid present in large amounts in urine but rarely detected in serum or most organs/tissues examined.Quantitative analyses of urinary l-β-ehasn in young healthy volunteers revealed significant correlation between urinary l-β-ehasn concentration and creatinine level.Further, for in-depth analyses of l-β-ehasn, we developed a simple three-step synthetic method using trans-epoxysuccinic acid as the starting substance.In addition, our research revealed a strong inhibitory effect of l-β-ehasn on mammalian serine racemase, responsible for producing d-serine, a co-agonist of the n-methyl-d-aspartate (nmda) receptor involved in glutamatergic neurotransmission.

Journal: Bioscience reports (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Multiple pathways are responsible to the inhibitory effect of butorphanol on OGD/R-induced apoptosis in AC16 cardiomyocytes.

Ischemic heart disease is the leading cause of cardiovascular mortality, which is related to cardiac myocyte apoptosis.Butorphanol is an opioid receptor agonist with potential cardioprotective function.The purpose of this work is to explore the function and mechanism of butorphanol in oxygen and glucose deprivation/reperfusion (ogd/r)-induced cardiomyocyte apoptosis.The overlapping targets of ischemic heart disease and butorphanol were analyzed according to gene cards, parm mapper, cytoscape, and kyoto encyclopedia of genes and genomes (kegg) pathway enrichment analysis.Human cardiomyocyte ac16 cells were incubated with butorphanol and then stimulated with ogd/r.Cell injury was investigated by cell counting kit-8, lactate dehydrogenase (ldh) assay kit, tunel staining, caspase-3 activity assay kit, and western blotting.The proteins in signaling pathways were measured using western blotting.A total of 93 overlapping targets of ischemic heart disease and butorphanol were obtained.Pathway analysis exhibited that these targets might be involved in multiple signaling pathways.Butorphanol alone showed little cytotoxicity to cardiomyocytes, and it protected against ogd/r-induced viability inhibition, ldh release, cell apoptosis, and increase of caspase-3 activity and expression levels of cleaved caspase-3 and bim.Butorphanol promoted the activation of the phosphoinositide 3-kinase (pi3k)/protein kinase b (akt)/forkhead box o (fox o) and hypoxia-inducible factor-1α (hif-1α)/vascular endothelial growth factor (vegf) pathways and attenuated the activation of the mitogen-activated protein kinase (mapk) signaling in ogd/r-treated cardiomyocytes.In conclusion, butorphanol prevents ogd/r-induced cardiomyocyte apoptosis through activating the pi3k/akt/fox o and hif-1α/vegf pathways and inactivating the mapk pathway.

Journal: Journal of applied toxicology : JAT (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Detection of Corneal Ectasia Using OCT Maps of Pachymetry and Posterior Surface Mean Curvature.

To quantify the abnormal corneal thinning and posterior surface steepening that is observed in keratoconus with an ectasia index.Methods optical coherence tomography (oct) was used to image the corneas of normal individuals and patients with varying stages of keratoconus (manifest, subclinical, and forme fruste).Maps of corneal pachymetry and posterior surface mean curvature were generated, and an ectasia index was calculated by multiplying gaussian fits obtained from the two types of maps.Repeated five-fold cross-validation was used to evaluate the ability of the ectasia index to differentiate between normal and keratoconic eyes.The classification performance of the ectasia index was compared to minimum pachymetry and maximum posterior mean curvature.Results thirty-two eyes from 16 normal individuals, 89 eyes from 63 patients with manifest keratoconus, 16 eyes from 15 patients with subclinical keratoconus, and 26 eyes from 26 patients with forme fruste keratoconus were included in the study.During cross-validation, 100% of the eyes with manifest (89 of 89) and subclinical (16 of 16) keratoconus were correctly classified by the ectasia index.The average classification accuracy for the forme fruste keratoconus group was 63 ± 21% (16.4 of 26).The specificity for the normal group was 91 ± 10% (29.1 of 32).The ectasia index had a higher sensitivity for keratoconus detection and similar specificity in comparison to minimum pachymetry and maximum posterior mean curvature.Conclusions the ectasia index could be a valuable additional metric for clinicians to consider when screening for keratoconus.[j refract surg.2022;38(8):502-510.].

Journal: Journal of refractive surgery (Thorofare, N.J. : 1995) (2022)
Publication types: Journal Article
Autonomic dysfunction and white matter microstructural changes in drug-naïve patients with Parkinson's disease.

Autonomic dysfunction (ad) is one of the non-motor features of parkinson's disease (pd).Some symptoms tend to occur in the early stages of pd.Ad also has a great impact on patient's quality of life.In this study, we aimed to discover the association between ad (scales for outcomes in parkinson's disease-autonomic, scopa-aut) and microstructural changes in white matter tracts in drug-naïve early pd patients to elucidate the central effects of autonomic nervous system impairments.Method in total, this study included 85 subjects with pd recruited from the parkinson's progression markers initiative (ppmi) database.Among the 85 pd patients, 38 were in hoehn & yahr stage 1 (hy1pd) and 47 were in stage 2 (hy2pd).Diffusion magnetic resonance imaging (dmri) data were reconstructed in the mni space using q-space diffeomorphic reconstruction to obtain the spin distribution function.The spin distribution function (sdf) values were used in dmri connectometry analysis.We investigated through diffusion mri connectometry the structural correlates of white matter tracts with scopa-aut subscores and total score.Results connectometry analysis also revealed positive association with white matter density in bilateral corticospinal tract in hy1pd patients and negative association in genu of corpus callosum (cc) and, bilateral cingulum in both groups.In addition, there were associations between gastrointestinal, sexual, thermoregulatory and urinary items and structural brain connectivity in pd.Conclusion our study reveals positive correlation, suggesting neural compensations in early pd.Cingulum and cc tracts have well-known roles in pd pathology, compatible with our findings that bring new insights to specific areas of ad and its role in central nervous system (cns) neurodegeneration, paving the way for using prodromal makers in the diagnosis and treatment of pd.

Journal: PeerJ (2018)
Publication types: Journal Article
SAR442085, a novel anti-CD38 antibody with enhanced antitumor activity against multiple myeloma.

Anti-cd38 monoclonal antibodies (m abs) represent a breakthrough in the treatment of multiple myeloma (mm), yet some patients fail to respond or progress quickly with this therapy, highlighting the need for novel approaches.In this study we compared the preclinical efficacy of sar442085, a next-generation anti-cd38 m ab with enhanced affinity for activating fcγ receptors (fcγr), with first-generation anti-cd38 m ab daratumumab and isatuximab.In surface plasmon resonance and cellular binding assays, we found that sar442085 had higher binding affinity than daratumumab and isatuximab for fcγriia (cd32a) and fcγriiia (cd16a).Sar442085 also exhibited better in vitro antibody-dependent cellular cytotoxicity (adcc) against a panel of mm cells expressing variable cd38 receptor densities including mm patients' primary plasma cells.The enhanced adcc of sar442085 was confirmed using nk-92 cells bearing low and high affinity fcγriiia (cd16a)-158f/v variants.Using mm patients' primary bone marrow cells, we confirmed that sar442085 had an increased ability to engage fcγriiia, resulting in higher natural killer (nk) cell activation and degranulation against primary plasma cells than preexisting fc wild-type anti-cd38 m abs.Finally, using hu fcg r transgenic mice that express human fcγ receptors under the control of their human regulatory elements, we demonstrated that sar442085 had higher nk cell-dependent in vivo antitumor efficacy and better survival than daratumumab and isatuximab against el4 thymoma or vk*myc myeloma cells overexpressing human cd38.These results highlight the preclinical efficacy of sar442085 and support the current evaluation of this next-generation anti-cd38 antibody in phase i clinical development in patients with relapsed/refractory mm.

Journal: Blood (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Immunoexpression of Ki67, p53 and cyclin D1 in osteosarcomas.

The main malignant tumor of the bone tissue is represented by osteosarcoma, neoplasia with a reserved prognosis and an unpredictable evolution, often aggressive.Cell cycle disruption is one of the complex biomolecular mechanisms involved in the progression of osteosarcomas.In this study, we analyzed the immunoexpression of ki67, p53 and cyclin d1 for 18 primitive osteosarcomas in relation to the clinicopathological prognosis parameters of the lesions.The results indicated the predominance of lesions in male young patients, with femoral location, most tumors being represented by the osteoblastic type, with high grade, size <8 cm and in advanced stages.Reactions were present in all cases, the high immunoexpression being associated with osteoblastic∕epithelioid types (ki67, cyclin d1, p53), high grade (ki67, cyclin d1) and advanced stage (ki67, cyclin d1).The study revealed a positive linear relation of the investigated immunomarkers expression, which indicates their usefulness in identifying lesions with aggressive progression potential.

Journal: Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (2021)
Publication types: Journal Article
A Survey on Analog-to-Digital Converter Integrated Circuits for Miniaturized High Resolution Ultrasonic Imaging System.

As traditional ultrasonic imaging systems (uis) are expensive, bulky, and power-consuming, miniaturized and portable uis have been developed and widely utilized in the biomedical field.The performance of integrated circuits (ics) in portable uis obviously affects the effectiveness and quality of ultrasonic imaging.In the ics for uis, the analog-to-digital converter (adc) is used to complete the conversion of the analog echo signal received by the analog front end into digital for further processing by a digital signal processing (dsp) or microcontroller unit (mcu).The accuracy and speed of the adc determine the precision and efficiency of uis.Therefore, it is necessary to systematically review and summarize the characteristics of different types of adcs for uis, which can provide valuable guidance to design and fabricate high-performance adc for miniaturized high resolution uis.In this paper, the architecture and performance of adc for uis, including successive approximation register (sar) adc, sigma-delta (σ-∆) adc, pipelined adc, and hybrid adc, have been systematically introduced.In addition, comparisons and discussions of different types of adcs are presented.Finally, this paper is summarized, and presents the challenges and prospects of adc ics for miniaturized high resolution uis.

Journal: Micromachines (2022)
Publication types: Journal Article;Review
GpsB Promotes PASTA Kinase Signaling and Cephalosporin Resistance inEnterococcus faecalis.

Enterococci are opportunistic pathogens that can cause severe bacterial infections.Treatment of these infections is challenging because enterococci possess intrinsic and acquired mechanisms of resistance to commonly used antibiotics, including cephalosporins.The transmembrane serine/threonine pasta kinase, ire k, is an important determinant of enterococcal cephalosporin resistance.Upon exposure to cephalosporins, ire k becomes autophosphorylated, which stimulates its kinase activity to phosphorylate downstream substrates and drive cephalosporin resistance.However, the molecular mechanisms that modulate ire k autophosphorylation in response to cell wall stress, such as that induced by cephalosporins, remain unknown.A cytoplasmic protein, gps b, promotes signaling by pasta kinase homologs in other bacterial species, but the function of enterococcal gps b has not been previously investigated.We usedin vitroandin vivoapproaches to test the hypothesis that enterococcal gps b promotes ire k signaling in response to cephalosporins to drive cephalosporin resistance.We found that gps b promotes ire k activity bothin vivoandin vitro.This effect is required for cephalosporins to trigger ire k autophosphorylation and activation of an ire k-dependent signaling pathway, and thereby is also required for enterococcal intrinsic cephalosporin resistance.Moreover, analyses of gps b mutants and a δire k gps bdouble mutant suggest that gps b has an additional function, beyond regulation of ire k activity, which is required for optimal growth and full cephalosporin resistance.Collectively, our data provide new insights into the mechanism of signal transduction by the pasta kinase ire k and the mechanism of enterococcal intrinsic cephalosporin resistance.importanceenterococci are opportunistic pathogens that can cause severe bacterial infections.Treatment of these infections is challenging because enterococci possess intrinsic and acquired resistance to commonly used antibiotics.In particular, enterococci are intrinsically resistant to cephalosporin antibiotics, a trait that requires the activity of a transmembrane serine/threonine kinase, ire k, which belongs to the bacterial pasta kinase family.The mechanisms by which pasta kinases are regulated in cells are poorly understood.Here, we report that the cytoplasmic protein gps b directly promotes ire k signaling in enterococci to drive cephalosporin resistance.Thus, we provide new insights into pasta kinase regulation and control of enterococcal cephalosporin resistance, and suggest that gps b could be a promising target for new therapeutics to disable cephalosporin resistance.

Journal: Journal of bacteriology (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural
Hounsfield unit measurement method and related factors that most appropriately reflect bone mineral density on cervical spine computed tomography.

Our study's purpose was to determine the most reliable hounsfield unit (hu) measurement method to reflect bone mineral density (bmd) on cervical spine computed tomography (ct) and to identify any factors that influence these results.Materials and methods we retrospectively analyzed 439 consecutive patients with mild head and neck injuries.Mean hu values of the c2-c7 vertebra were determined on each sagittal, coronal, and axial ct image.Correlation patterns were analyzed between the hu value and corresponding dual-energy x-ray absorptiometry (dxa) in the lumbar vertebra (t-score) and femoral neck (t-score).A sub-group analysis was performed according to patient age, sex, and degree of spinal degeneration.Results the correlation coefficients for hu and dxa ranged from 0.52 to 0.65 in all cervical segments.A simple linear regression analysis revealed the following formula: t-score = 0.01 × (hu) - 4.55.The mean hu values for osteopenia and osteoporosis were 284.0 ± 63.3 and 231.5 ± 52.8, respectively.The roc curve indicated that the hu method has a sensitivity of 89.2% and specificity of 88.7% to diagnose osteoporosis.The hu measurement showed a high correlation value (range: r = 0.64-0.70) with spine dxa score regardless of the degree of degeneration or patient age or sex.Conclusion hu values using the upper two cervical vertebrae (c2 and c3) reflected a more reliable bmd level than other segments.Additionally, the hu of cervical ct provided reliable information regardless of measurement plane, age or sex, and degree of degeneration.

Journal: Skeletal radiology (2022)
Publication types: Journal Article
Surgical treatment options for benign prostatic obstruction: beyond prostate volume.

Of review many surgical treatment options are available for patients who present with benign prostatic obstruction (bpo).This article reviews the current treatment options available and distinguishes them based on five clinical considerations: antithrombotic therapy, sexual function preservation, ambulatory procedures, anesthesia-related risks and duration of catheterization.Recent findings a comprehensive review of the literature was performed on 10 bpo procedures.Laser enucleation of the prostate (lep), bipolar plasma transurethral vaporization of the prostate and photoselective vaporization (pvp) of the prostate reduces the risk of bleeding, which is recommended for anticoagulated men.Ejaculatory function is more likely to be preserved following transurethral incision of the prostate, rezūm, aquablation, uro lift and i tind.Same-day discharge is possible for lep, pvp and prostatic arterial embolization (pae).For patients with high anesthesia-related risks, procedures compatible with local anesthesia (uro lift, rezūm, i tind and pae) should be favored.Catheterization duration is shorter with uro lift, pvp and lep.Summary bpo treatment options are growing rapidly.The optimal procedure for a given patient is based on factors such as associated risks, recovery and expected outcomes.Besides prostate volume, the clinical considerations in the present article can help elucidate the best surgical bpo treatment option for each patient based on their values, preferences, and risk tolerance.

Journal: Current opinion in urology (2022)
Publication types: Journal Article;Review