syedzayyan/paper_mill_benchmark huggingface dataset:
ProNGF/p75NTR Axis Drives Fiber Type Specification by Inducing the Fast-Glycolytic Phenotype in Mouse Skeletal Muscle Cells.

Despite its undisputable role in the homeostatic regulation of the nervous system, the nerve growth factor (ngf) also governs the relevant cellular processes in other tissues and organs.In this study, we aimed at assessing the expression and the putative involvement of ngf signaling in skeletal muscle physiology.To reach this objective, we employed satellite cell-derived myoblasts as an in vitro culture model.In vivo experiments were performed on tibialis anterior from wild-type mice and an mdx mouse model of duchenne muscular dystrophy.Targets of interest were mainly assessed by means of morphological, western blot and q rt-pcr analysis.The results show that pro ngf is involved in myogenic differentiation.Importantly, the pro ngf/p75ntr pathway orchestrates a slow-to-fast fiber type transition by counteracting the expression of slow myosin heavy chain and that of oxidative markers.Concurrently, pro ngf/p75ntr activation facilitates the induction of fast myosin heavy chain and of fast/glycolytic markers.Furthermore, we also provided evidence that the oxidative metabolism is impaired in mdx mice, and that these alterations are paralleled by a prominent buildup of pro ngf and p75ntr.These findings underline that the pro ngf/p75ntr pathway may play a crucial role in fiber type determination and suggest its prospective modulation as an innovative therapeutic approach to counteract muscle disorders.

Journal: Cells (2020)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Resistance to Beta-cypermethrin, Azadirachtin, and Matrine, and Biochemical Characterization of Field Populations of Oedaleus asiaticus (Bey-Bienko) in Inner Mongolia, Northern China.

Oedaleus asiaticus (bey-bienko) is an economically devastating locust species found in grassland and pastoral areas of the inner mongolia region of northern china.In this study, resistance to three frequently used insecticides (beta-cypermethrin, matrine, and azadirachtin) was investigated in six field populations of o.Asiaticus using the leaf-dip bioassay method.The inhibitory effects of synergists and the activities of detoxification enzyme activities in the different populations were determined to explore potential biochemical resistance mechanisms.The results showed that the field populations sb (resistance ratio [rr] = 7.85), zb (rr = 5.64), and db (rr = 6.75) had developed low levels of resistance to beta-cypermethrin compared with a susceptible control strain.Both the sb (rr = 5.92) and xc (rr = 6.38) populations had also developed low levels of resistance against matrine, with the other populations remaining susceptible to both beta-cypermethrin and matrine.All field populations were susceptible to azadirachtin.Synergism analysis showed that triphenyl phosphate (tpp) and diethyl-maleate (dem) increased the toxicity of beta-cypermethrin significantly in the sb population, while the synergistic effects of tpp, piperonyl butoxide (pbo), and dem on the toxicity of matrine were higher in sb (sr 3.86, 4.18, and 3.07, respectively) than in ss (sr 2.24, 2.86, and 2.29, respectively), but no synergistic effects of tpp, pbo, and dem on azadirachtin were found.Biochemical assays showed that the activities of carboxylesterases (car es) and glutathione-s-transferases (gsts) were significantly raised in all field populations of o.Asiaticus, with a significant positive correlation observed between beta-cypermethrin resistance and car e activity.The activities of cytochrome p450 monooxygenases (p450) and multi-function oxidases (mfo) were elevated in all six field populations, and p450 activity displayed strong positive correlations with the three insecticides.Our findings suggest that resistance to beta-cypermethrin in o.Asiaticus may be mainly attributed to elevated car e and gst activities, while p450 plays an important role in metabolizing matrine and azadirachtin.Our study provides insights that will help improve insecticide resistance management strategies.

Journal: Journal of insect science (Online) (2022)
Publication types: Journal Article
Greater trochanteric pain after primary total hip replacement, comparing the anterior and posterior approach: A secondary analysis of a randomized trial.

Greater trochanteric pain (gtp) after total hip replacement is a common cause of residual lateral hip pain, regardless of the approach used.The goal of our study was to evaluate gtp after a direct anterior approach (daa) compared to a posterior approach (pa) as well as the clinical outcomes of both approaches and answer the following: 1) what is the incidence of trochanteric pain after primary tha with two different surgical approaches?2) what is the functional outcome of patients with gtp?3) what proportion of patients with gtp resort to peritrochanteric injections?Hypothesis our hypothesis is that gtp is present with both approaches but satisfaction is lower with the pa.Patients and methods a secondary analysis of a previously published clinical trial with 55 total hip arthroplasty patients randomized in one of two surgical approaches: 27 patients underwent the anterior modified hueter approach, while the other group of 28 patients were operated using the posterior approach.Study outcomes were modified harris hip score (mhhs), satisfaction score, pain when lying on the affected side, and requiring an injection.Hip offset, femur lateralization and leg lengthening were measured before and after surgery.Results forty-five patients were available for complete follow-up at a mean of 62 months (range: 48-74).The incidence of gtp was higher in the posterior approach [pa: 6/21 (29%) vs daa 4/24 (17%)) (p=0.3).Patients operated through a pa experienced more pain [5/21 (24%) of patients; vas=mean 5.3] when lying on their operated side, compared to daa patients [2/24 (8%) of patients; vas=mean 2) (p=0.2)].However, mhhs, patient satisfaction with surgery, radiological assessment for hip offset, femur lateralization or leg lengthening, and injections required were similar for both approaches.Overall, satisfaction and functional outcome with surgery was significantly lower in gtp patients, regardless of the approach.Conclusions gtp impacts patient satisfaction and functional outcome in total hip arthroplasty patients.Pa patients reported more trochanteric pain than daa patients, which affected their clinical outcome.Level of evidence iii.

Journal: Orthopaedics & traumatology, surgery & research : OTSR (2021)
Publication types: Journal Article;Randomized Controlled Trial;Research Support, Non-U.S. Gov't
Split Tolerance in a Murine Model of Heterotopic En Bloc Chest Wall Transplantation.

Congenital and acquired chest wall deformities represent a significant challenge to functional reconstruction and may impact feasibility of heart transplantation for patients with end-stage organ failure.In the recent past, the concept of replacing like-with-like tissue by using vascularized composite allografts (vca) has been enthusiastically employed for reconstruction of complex tissue defects.Methods in this study, we introduce a novel murine model for en bloc chest wall, heart, and thymus transplantation and thereby the use of complex tissue allografts for reconstruction of both chest wall defects and also end-stage organ failure.Additionally, this model allows us to study the features of combined vascularized bone marrow (vbm), thymus, and heart transplantation on allograft survival and function.Heterotopic chest wall, thymus, and heart transplants were performed in untreated syngeneic and allogeneic combinations and in allogeneic combinations treated with costimulation blockade (ctla4-ig and mr-1).Results indefinite (ie, 150 d,n= 3) graft survival was observed in syngeneic controls.In untreated recipients of allogeneic grafts, the skin component was rejected after 10 (±1) days, whereas rejection of the heart occurred after 13 (± 1) days (n= 3).Costimulation blockade treatment prolonged survival of the heart and chest wall component (130 d,n= 3) as well as the vbm niche as evidenced by donor-specific chimerism (average: 2.35 ± 1.44%), whereas interestingly, the skin component was rejected after 13 (±1) days.Conclusion thus, this novel microsurgical model of vca combined with solid organ transplantation is technically feasible and results in split tolerance when treated with costimulatory blockade.

Journal: Plastic and reconstructive surgery. Global open (2017)
Publication types: Journal Article
Longest survival with primary intracranial malignant melanoma: A case report and literature review.

Primary intracranial malignant melanoma (pimm) is rare, and its prognosis is very poor.It is not clear what systematic treatment strategy can achieve long-term survival.This case study attempted to identify the optimal strategy for long-term survival outcomes by reviewing the pimm patient with the longest survival following comprehensive treatment and by reviewing the related literature.Case summary the patient is a 47-year-old chinese man who suffered from dizziness and gait disturbance.He underwent surgery for right cerebellum melanoma and was subsequently diagnosed by pathology in june 2000.After the surgery, the patient received three cycles of chemotherapy but relapsed locally within 4 mo.Following the second surgery for total tumor resection, the patient received an injection of newcastle disease virus-modified tumor vaccine, interferon, and β-elemene treatment.The patient was tumor-free with a normal life for 21 years before the onset of the recurrence of melanoma without any symptoms in july 2021.A third gross-total resection with adjuvant radiotherapy and temozolomide therapy was performed.Brain magnetic resonance imaging showed no residual tumor or recurrence 3 mo after the 3rdoperation, and the patient recovered well without neurological dysfunction until the last follow-up in june 2022, which was 22 years following the initial treatment.Conclusion it is important for patients with pimm to receive comprehensive treatment to enable the application of the most appropriate treatment strategies.Long-term survival is not impossible in patients with these malignancies.

Journal: World journal of clinical cases (2022)
Publication types: Case Reports;Journal Article
Germinal center derived B cell memory without T cells.

Liu et al.(2022.J.Exp.Med.In this issue show that t cell-independent germinal centers (gcs) can produce long-lived memory and plasma cell output.This may help explain how polysaccharide antigens provide long-term protection.

Journal: The Journal of experimental medicine (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't;Comment
Analysis of features of social anxiety and exploring the relationship between childhood major adverse experiences and social anxiety in early adulthood among Chinese college students.

Introduction prior studies have explored the prevalence of social anxiety disorders and found that childhood major adverse experiences increased the risk of social anxiety.However, few studies analyzed features of social anxiety and explored the mediation mechanism of the relationship between childhood major adverse experiences and social anxiety in early adulthood.Methods two thousand seven hundred and fifty-nine college students from hunan, china participated in the survey-based study.They completed questionnaires on major adverse childhood experiences, social anxiety symptoms, and a sense of security during a study conducted from 2017 to 2018.Results 1) 33.38% of the students reported experiencing at least one social anxiety symptom; the highest detection rate (20.22%) was social anxiety symptoms related to participants' nervousness when in large groups.2) females and individuals who consumed alcohol in the past year and individuals with a poor physical condition had a significantly higher risk of having social anxiety symptoms (p<0.05).3) childhood major adverse events experience increased the risk of some social anxiety symptoms, especially experiencing 3 or more adverse events (p<0.05).4) a sense of security mediated the relationship between childhood major adverse events experience and social anxiety symptoms.Conclusions extensive detection rate of social anxiety symptoms existed among college students, early adversities increased the risk of social anxiety in early adulthood, and the relationship between childhood major adversity and social anxiety was affected by sense of security.

Journal: Journal of affective disorders (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Otologic Manifestations of Psoriasis: A Systematic Review and Meta-Analysis.

Introduction psoriatic skin lesions are often seen in the auricle and external auditory canal, but middle ear and inner ear manifestations are not easily identified.Several studies have indicated hearing loss and vestibular dysfunction with psoriasis, but the extent of association has not been well defined.Methods a systematic search of pub med, cinahl, scopus, and cochrane library was conducted following preferred reporting items for systematic reviews and meta-analyses guidelines.Included studies described audiometric or vestibular assessment of psoriasis patients.Meta-analysis was represented with odds ratios (ors) or mean difference (md) with 95% confidence intervals.Results a total of 13 studies with 589 psoriasis patients and 617 healthy controls were included.Age did not significantly differ between psoriasis patients (47.1 [12.4] yr) and healthy controls (45.4 [11.2] yr).Psoriasis area and severity index score was 9.9 (8.4), and body surface area scores was 7.9 (14.7).Pure-tone audiometry analysis showed higher mean hearing thresholds in psoriasis patients compared with healthy controls across all frequencies, with the greatest difference at 4000 hz (md, 7.70 [4.46-10.94]; p < 0.00001).Speech reception thresholds were worse with psoriasis (md, 3.53 [1.56-5.49]; p < 0.0001).Abnormal stapedial reflex was more common in psoriasis (or, 5.19 [1.68-15.99]; p = 0.004).Abnormal vestibular testing was more common in psoriasis for caloric testing (or, 13.12 [2.88-59.84]; p < 0.0001).Two additional studies of 41,681 psoriasis patients and 80,273 healthy controls found that psoriasis patients were at higher risk for sudden sensorineural hearing loss (or, 1.50 [1.25-1.80]; p < 0.0001).Conclusion our study shows that psoriasis is associated with hearing loss and vestibular dysfunction, but clinical significance remains undefined.The trends noted in our study require more investigation, and the pathophysiologic mechanisms need to be defined.

Journal: Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology (2022)
Publication types: Journal Article;Meta-Analysis;Systematic Review
Tan's two-body contact across the superfluid transition of a planar Bose gas.

Tan's contact is a quantity that unifies many different properties of a low-temperature gas with short-range interactions, from its momentum distribution to its spatial two-body correlation function.Here, we use a ramsey interferometric method to realize experimentally the thermodynamic definition of the two-body contact, i.e., the change of the internal energy in a small modification of the scattering length.Our measurements are performed on a uniform two-dimensional bose gas of87rb atoms across the berezinskii-kosterlitz-thouless superfluid transition.They connect well to the theoretical predictions in the limiting cases of a strongly degenerate fluid and of a normal gas.They also provide the variation of this key quantity in the critical region, where further theoretical efforts are needed to account for our findings.

Journal: Nature communications (2021)
Publication types: Journal Article
Effect of 5-Azacitidine Treatment on Redox Status and Inflammatory Condition in MDS Patients.

This study focused on the impact of the treatment with the hypomethylating agent 5-azacitidine on the redox status and inflammation in 24 mds patients.Clinical and genetic features of mds patients were recorded, and peripheral blood samples were used to determine the activity of the endogenous antioxidant defense system (superoxide dismutase, sod; catalase, cat; glutathion peroxidase, gpx; and reductase, grd, activities), markers of oxidative damage (lipid peroxidation, lpo, and advanced oxidation protein products, aopp).Moreover, pro-inflammatory cytokines and plasma nitrite plus nitrate levels as markers of inflammation, as well as co q10 plasma levels, were also measured.Globally, mds patients showed less redox status in terms of a reduction in the gssg/gsh ratio and in the lpo levels, as well as increased cat activity compared with healthy subjects, with no changes in sod, gpx, and grd activities, or aopp levels.When analyzing the evolution from early to advanced stages of the disease, we found that the gpx activity, gssg/gsh ratio, lpo, and aopp increased, with a reduction in cat.Gpx changes were related to the presence of risk factors such as high-risk ipss-r or mutational score.Moreover, there was an increase in il-2, il-6, il-8, and tnf-α plasma levels, with a further increase of il-2 and il-10 from early to advanced stages of the disease.However, we did not observe any association between inflammation and oxidative stress.Finally, 5-azacitidine treatment generated oxidative stress in mds patients, without affecting inflammation levels, suggesting that oxidative status and inflammation are two independent processes.

Journal: Antioxidants (Basel, Switzerland) (2022)
Publication types: Journal Article
Co-Delivery of Repurposing Itraconazole and VEGF siRNA by Composite Nanoparticulate System for Collaborative Anti-Angiogenesis and Anti-Tumor Efficacy against Breast Cancer.

Combinations of two different therapeutic modalities of vegf inhibitors against angiogenesis can cooperatively impede breast cancer tumor growth and enhance therapeutic efficacy.Itraconazole (itz) is a conventional antifungal drug with high safety; however, it has been repurposed to be a multi target anti-angiogenesis agent for cancer therapy in recent years.In the present study, composite nanoparticles co-loaded with itz and vegf si rna were prepared in order to investigate their anti-angiogenesis efficacy and synergistic anticancer effect against breast cancer.The nanoparticles had a suitable particle size (117.9 ± 10.3 nm) and weak positive surface charge (6.69 ± 2.46 m v), as well as good stability and drug release profile in vitro.Moreover, the nanoparticles successfully escaped from endosomes and realized cell apoptosis and cell proliferation inhibition in vitro.In vitro and in vivo experiments showed that the nanoparticles could induce the silencing of vegf-related expressions as well as anti-angiogenesis efficacy, and the co-loaded itz-vegf si rna nps could inhibit tumor growth effectively with low toxicity and side effects.Taken together, the as-prepared delivery vehicles are a simple and safe nano-platform that improves the antitumor efficacy of vegf si rna and itz, which allows the repositioning of the generic drug itz as a great candidate for antitumor therapy.

Journal: Pharmaceutics (2022)
Publication types: Journal Article
Conducting clinical research in the era of the COVID-19 pandemic: Challenges and lessons for Speech-Language Pathology and Audiology research.

The novel coronavirus disease 2019 (covid-19) presented new and unanticipated challenges to the academic training and performance of clinical research at undergraduate and postgraduate levels of training.This highlighted the need for reimagining research designs and methods to ensure continued generation of knowledge - a core function of a research-intensive university.Whilst adhering to government regulations geared towards protecting both the research participants and researchers, innovative research methods are required.Objective the purpose of this scoping review is to explore published evidence on innovative clinical research methods and processes employed during covid-19 and to document challenges encountered and lessons that the fields of speech-language pathology and audiology can learn.Methods electronic bibliographic databases including science direct, pub med, scopus, medline, pro quest were searched to identify peer-reviewed publications, published in english, between 2019 and 2021, related to innovative clinical research methods and processes applied where in-person contact is regulated.Results significant challenges with conducting research in the covid-19 era were identified, with important lessons learned and numerous opportunities that have relevance for this pandemic era and beyond.These findings are presented under 10 themes that emerged that highlight important considerations for research methods and processes during a pandemic and beyond.The findings of this study also raise implications for telehealth from which low- and middle-income countries (lmics), where resource challenges exist, can benefit.Conclusion challenges and opportunities identified in this review have relevance for the field of speech-language pathology and audiology as far as current and future (beyond covid-19) clinical research planning is concerned.

Journal: The South African journal of communication disorders = Die Suid-Afrikaanse tydskrif vir Kommunikasieafwykings (2022)
Publication types: Journal Article;Review
Effects of UPF1 expression on EMT process by targeting E‑cadherin, N‑cadherin, Vimentin and Twist in a hepatocellular carcinoma cell line.

Hepatocellular carcinoma (hcc) is one of the most common malignancies worldwide.It has been reported that hcc has a poor prognosis.In the majority of cases, once metastatic, hcc is incurable.To identify an effective treatment for hcc, it is important to understand the underlying molecular mechanisms of hcc‐associated occurrence, proliferation, metastasis and carcinogenesis.In the present study, the role of up‐frameshift 1 (upf1), a potential tumor suppressor, was investigated in the hcc cell lines.The expression levels of upf1 in an hcc cell line were examined by reverse transcription‐quantitative polymerase chain reaction.The expression levels of 19 key proteins in numerous signaling pathways were detected via protein array analysis in the presence of upf1 overexpression.The present study further investigated the effects of upf1 expression levels on the epithelial‐mesenchymal transition (emt) process by targeting e‐cadherin, n‐cadherin, vimentin and twist‐related protein 1 (twist).The results of the present study revealed that upf1 was significantly downregulated in an hcc cell line.The majority of the proteins exhibited upregulated expression levels in the presence of upf1 overexpression in the hcc cell line, huh‐7.Key proteins, including cluster of differentiation (cd)31 (platelet endothelial cell adhesion molecule‐1), vimentin, cd44, pcna, ki‐67, n‐cadherin, survivin, p53, met and retinoblastoma exhibited a significant association with upf1.Furthermore, western blotting indicated that the expression levels of n‐cadherin, vimentin and twist were notably upregulated while upf1 was overexpressed; however, e‐cadherin was downregulated and opposing observations were reported with protein array analysis.In summary, e‐cadherin expression levels were regulated by the manifold, and upf1, a potential tumor suppressor, may promote the emt process in huh‐7 hcc cells.The findings of the present study suggested that upf1 expression levels affected the emt process by targeting e‐cadherin, n‐cadherin, vimentin and twist.

Journal: Molecular medicine reports (2019)
Publication types: Journal Article