syedzayyan/paper_mill_benchmark huggingface dataset:
Role of household waste, governance quality, and greener energy for public health: Evidence from developed economies.

In the current times, the global economies and international organizations declared that pollution is one of the prominent causes of declined human health.Still, most literature is biased toward economic sustainability and ignores such vital issues.The current study tends to identify the factors affecting public health in the group of seven economies except for italy (g6).Specifically, this study aims to investigate the influence of household waste (hhw), bureaucratic quality (bq), democratic accountability (da), urbanization growth (urp), gdp per capita, and renewable energy use (epr) on public health, throughout 1996-2020.This study uses advanced panel data approaches and finds the heterogeneity of slope coefficients, the dependence of cross-sections, and the persistence of cointegration between the variables.The asymmetric distribution of data leads to employing the novel method of moment quantile regression.The estimated results reveal that urp, gdppc, and epr significantly increase domestic general government health expenditures, improving public health.However, hhw and bq adversely affect public health by reducing health expenditures.The robustness of the results is testedviautilizing the panel quantile regression.Based on the empirical findings, this study suggests policies regarding the improvement in public health expenditure, r&d investment, spending in renewable energy sector, and strengthening of the institutional quality.

Journal: Frontiers in public health (2022)
Publication types: Journal Article
Luteolin binds Src, promotes STAT3 protein ubiquitination and exerts anti-melanoma effects in cell and mouse models.

Signal transducer and activator of transcription 3 (stat3) has been proposed as a target for melanoma prevention.Luteolin, a bioactive flavonoid abundant inmedicinal herbs, has been reported to have anti-melanoma activity in vitro.However, its in vivo anti-melanoma effects and underlying mechanisms have not been fully elucidated.In this study, ten cell lines and two mouse models (b16f10 allograft and a375 xenograft models) were used for assessing the in vitro and in vivo anti-melanoma effects of luteolin.A stat3 over-activated stable a375 cell line was used to determine the contribution of stat3 signaling in luteolin's anti-melanoma effects.Results showed that luteolin dose-dependently reduced viability of melanoma cells.Luteolin also induced apoptosis in, and suppressed migration and invasion of, a375 and b16f10 melanoma cells.Mechanistically, luteolin inhibited phosphorylation of stat3 and src (an upstream kinase of stat3), accelerated ubiquitin-proteasome pathway-mediated stat3 degradation, and downregulated the expression of stat3-targeted genes involved in cell survival and invasion in melanoma cells.Molecular modelling and surface plasmon resonance imaging showed that luteolin stably bound to the protein kinase domain of src.Animal studies demonstrated that prophylactic administration of luteolin restrained melanoma growth and src/stat3 signaling in both a375 and b16f10 melanoma-bearing mice.Moreover, luteolin's anti-melanoma effects were diminished by stat3 over-activation in a375 cells.Our findings indicate that luteolin inhibits stat3 signaling by suppressing stat3 activation and promoting stat3 protein degradation in melanoma cells, thereby exhibiting anti-melanoma effects.This study provides further pharmacological groundwork for developing luteolin as a chemopreventive agent against melanoma.

Journal: Biochemical pharmacology (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Determining nonesterified and total docosahexaenoic acid and eicosapenaenoic acid concentrations by LC-MS/MS in the plasma of patients with schizophrenia.

Nonesterified, total docosahexaenoic acid (dha) and eicosapenaenoic acid (epa) plasma levels were evaluated in patients with schizophrenia on different medications compared with healthy individuals using validated lc-ms/ms methods.methods:samples for nonesterified dha and epa assay were extracted in n-hexane-dichloromethane-isopropyl alcohol (2:1:0.1, v/v/v) and hydrolyzed at 90°c for 2 h before total dha and epa determination.Methods were validated in surrogate matrix and plasma.results:these methods generated similar recovery for plasma (>89%) and surrogate matrix (>87%) and negligible matrix effects.Linearity, lower limit of quantification, accuracy, precision and stability were also validated.conclusions:this study successfully determined dha and epa plasma levels in patients with schizophrenia and healthy individuals using validated lc-ms/ms methods.Therefore, nonesterified dha and total epa levels could be used as schizophrenia biomarkers.

Journal: Bioanalysis (2022)
Publication types: Journal Article
Implementation fidelity of a Brazilian drug use prevention program and its effect among adolescents: a mixed-methods study.

Based on the us dare-ki r, a version of the keepin' it real program, the drug and violence resistance educational program (proerd) is the most widely implemented brazilian prevention program.It originates from the translation of the dare-ki r, a version of the keepin' it real program.Previous results suggest its inefficiency in preventing drug use among brazilian adolescents.Since ki r fidelity can impact program outcomes, this mixed-methods study evaluates the proerd implementation fidelity and its effects on preventing drug use among adolescents.Methods data from two cluster randomized controlled trials (c rcts) with 4,030 students from 30 public schools in são paulo (1,727 fifth graders and 2,303 seventh graders), assessed at two-time points, were analyzed quantitatively.After implementing each lesson during the c rct, 19 proerd instructors answered fidelity forms.The effect of proerd fidelity on alcohol, cigarettes, marijuana, inhalant, and cocaine use (the last two only among seventh graders) in the six months prior to follow-up assessment was analyzed by logistic regressions for fifth grade and mixed effect models for seventh graders.For qualitative analysis, semi-structured interviews were conducted with proerd instructors and investigated by thematic analysis.Results quantitative analysis showed that proerd implementation fidelity had no impact on drug use among fifth and seventh graders.Conversely, the qualitative analysis revealed important aspects that may influence implementation fidelity and consequently program effectiveness, such as adaptations made by instructors, school infrastructure, among others, besides program application.Conclusion proerd requires cultural adaptation to improve its implementation in brazilian public schools.

Journal: Substance abuse treatment, prevention, and policy (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Invasive lobular carcinoma with extracellular mucin (ILCEM): clinicopathologic and molecular characterization of a rare entity.

Invasive lobular carcinoma with extracellular mucin (ilcem) is a rare histologic subtype of breast cancer.Little is known about the pathologic or genomic signatures that distinguish ilcem from classic invasive lobular carcinoma (ilc) or mucinous carcinoma.We studied 17 breast cancers with lobular morphology and extracellular mucin.Thirteen tumors with sufficient tissue for dna extraction were analyzed by a next generation sequencing (ngs) assay that interrogates 447 genes for mutations and copy number variations (cnvs).Median patient age was 66 yrs (range: 31-77 yrs).Sixteen patients presented with masses, 7 of which were >2 cm.Seven patients had lymph node metastases.The cases of ilcem were moderately (n = 13) or poorly differentiated (n = 4), frequently exhibiting variant morphology that has not been previously described or emphasized, including grade 3 nuclei (n = 11), diffuse signet ring cells (n = 10), solid growth (n = 4), tumor necrosis (n = 3) or apocrine features (n = 2).All tumors showed absent or reduced membranous e-cadherin expression.Concurrent lobular carcinoma in situ (lcis) was seen in 11/17 cases, 1 of which was a striking example of signet ring cell lcis with extracellular mucin.Receptor profiles were er+/her2- (n = 15) and er+/her2+ (n = 2).With a median follow-up of 83.5 months (range: 3-171 months) in 12 patients with available information, 8 patients had recurrences resulting in 4 cancer-related deaths.The most common cnvs were 16q loss (n = 11) and 1q gain (n = 9).Cdh1 gene-level alterations were detected in all but one case, including frameshift (n = 7), nonsense (n = 2), and donor splice site (n = 1) mutations and indels (n = 2).Recurrent mutations were also seen in pik3ca (n = 3), polq (n = 3), tp53 (n = 3), erbb3 (n = 3), erbb2 (n = 2), and runx1 (n = 2).Genes with recurrent amplifications included gata3 (n = 4), foxa1 (n = 3), ccnd1 (n = 2).Our data highlights ilcem as a distinct variant of ilc that often presents with higher-grade and variant morphologic features and is associated with an aggressive clinical course.Ngs data support an overall lobular-type molecular profile and reveal potentially targetable alterations in a subset of cases with recurrence.

Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (2022)
Publication types: Journal Article
The association of chronic endometritis with mid-trimester loss due to cervical incompetence and the outcome of laparoscopic cervical cerclage.

To examine the association of chronic endometritis (ce) with cervical incompetence (ci) in chinese women with mid-trimester loss, and the impact of the presence of ce on the outcome of laparoscopic cervical cerclage (lcc).Methods this retrospective cohort study included a study group of 293 women with mid-trimester loss due to ci (group i) and a comparison group of 332 women with recurrent first-trimester miscarriage (group ii).Immunohistochemical study using cd138 epitope for the diagnosis of ce was completed in all subjects.Pre-conception lcc was undertaken in 247 women in the study group (group i).The study was approved by institutional review board (irb) (number 2015fxhec-ky005).Results the prevalence of ce in group i was 42%, significantly (p < 0.001) higher than that of 23.5% in group ii.Among 247 women in group i, there were no significant difference in mid-trimester loss rate, preterm delivery rate and term delivery rate in women with and without ce (2.2, 12.0, 85.8% vs.1.8, 10.1, 88.1% respectively) and between women with ce treated and not treated with antibiotics prior to conception (2.3, 9.3, 88.4% vs.2.0, 14.3, 83.7% respectively).Conclusions mid-trimester loss due to cervical incompetence is associated with chronic endometritis; however, the presence or not of ce and whether it was treated with antibiotics prior to conception did not appear to significantly influence the obstetric outcomes of women with ci after lcc.

Journal: Archives of gynecology and obstetrics (2021)
Publication types: Journal Article
Circular RNA hsa_circ_0008896 accelerates atherosclerosis by promoting the proliferation, migration and invasion of vascular smooth muscle cells via hsa-miR-633/CDC20B (cell division cycle 20B) axis.

Circular rnas, a class of circularly closed non-coding rnas, play essential roles in the formation of atherosclerosis, which is a frequent cause of cardiovascular and cerebrovascular diseases.Although many circular rnas are found to be involved in the progression of atherosclerosis, more circular rna regulators still need to be identified, to improve the understanding of the regulatory networks of atherosclerosis.Here, we found that hsa_circ_0008896 was significantly up-regulated in bothin vitroandin vivoatherosclerosis models, indicating hsa_circ_0008896 was involved in the progression of atherosclerosis.Further functional analyses confirmed that knockdown of hsa_circ_0008896 decreased proliferation, migration, and invasion of vsmcs.In addition, we conducted bioinformatics analysis and found that hsa-mi r-633 could directly bind to hsa_circ_0008896, which was confirmed by rna immune-precipitation (rip) assays.Results of proliferation, migration, and invasion assays showed that hsa-mi r-633 inhibitor reversed the si-circ_0008896 phenotypes, indicating that hsa_circ_0008896 functionally bound to hsa-mi r-633.At last, combining bioinformatics and experimental analyses, we found the protein target of hsa_circ_0008896/hsa-mi r-633, cdc20b (cell division cycle 20b).The expression level of cdc20b was regulated by hsa-mi r-633, and knockdown of cdc20b decreased proliferation, migration, and invasion of vsmcs.Taken together, hsa_circ_0008896 regulated the expression of cdc20b by sponging hsa-mi r-633, and then enhanced proliferation, migration, and invasion of vsmcs to promote the progression of atherosclerosis.

Journal: Bioengineered (2022)
Publication types: Journal Article
Overproduction of rhamnolipid by fed-batch cultivation of Pseudomonas aeruginosa in a lab-scale fermenter under tight DO control.

Rhamnolipids are one of the most well-known classes of biosurfactants having wide applications in various industries due to low toxicity, high biodegradability, and environmentally friendly.Dissolved oxygen (do) concentration has the crucial effect on rhamnolipids production, particularly through fed-batch cultivation.In this study, the effect of different levels of do concentrations on rhamnolipid production by pseudomonas aeruginosa in both batch and fed-batch fermentation was investigated in a lab-scale fermenter under precise do control.A maximal rhamnolipid production of 22.5 g/l was obtained at a do concentration of 40% in batch fermentation.In order to achieve the high rhamnolipid production, a fed-batch operation under tight do control of 40% was conducted.As a result, the overall rhamnolipid production and productivity reached to 240 g/l and 0.9 (g/l h), corresponding to a 10.7 and 4.8-fold improvement compared to the batch experiments.The high level of rhamnolipid production via the fed-batch cultivation can be attributed to both do concentration and the feeding strategy.This achievement is promising for the production of rhamnolipid in industrial scale.

Journal: Biodegradation (2019)
Publication types: Journal Article
Ultrasonographic evaluation of lacrimal glands in patients with primary Sjögren's syndrome.

S this study focused on distinguishing the characteristic ultrasonographic findings of lacrimal glands in primary sjögren's syndrome (p ss) from those in idiopathic sicca syndrome.We aimed to set up a semi-quantitative scoring system of lacrimal gland ultrasonography (lgus) for patients with p ss.Methods fifty-six patients with p ss and 40 patients with idiopathic sicca syndrome were evaluated.Lacrimal glands were examined with ultrasonography using area, major/minor axis length, and five components (presence of intraglandular branch of lacrimal artery, inhomogeneity, hyperechoic bands, hypoechoic areas, and delineation).Except for the area and maximal/minimal length of lacrimal glands, other components were classified as dichotomous variables (present or absent).Using the receiver operating characteristics curve, we inferred the most appropriate combination of lgus scoring for p ss diagnosis.Results patients with p ss had a higher proportion of intraglandular branch of lacrimal artery (70.5% vs.42.5%, p<0.001), inhomogeneity (72.3% vs.46.3%, p<0.001), and hyperechoic bands (56.2% vs.37.5%, p=0.016) than patients with idiopathic sicca syndrome.Lgus a, which represents the summation of one point assigned for the presence of intraglandular branch of lacrimal artery and one for inhomogeneity, was the most suitable diagnostic criterion (area under curve = 0.724, 95% confidence interval 0.620-0.828).If both sides have a score of 2, it results in a total of 4 points.With a cut-off value of 3 out of 4 points, lgus a had 60.7% sensitivity, 71.1% specificity, 60.7% positive predictive value, and 72.5% negative predictive value.Conclusions semi-quantitative scoring of lgus was useful when distinguishing patients with p ss from those with idiopathic sicca syndrome.The combination of intraglandular branch of lacrimal artery and inhomogeneity on both sides was most suitable for classifying p ss using lgus.

Journal: Clinical and experimental rheumatology (2022)
Publication types: Journal Article
Numerical Simulations of Directed Self-Assembly in Diblock Copolymer Films using Zone Annealing and Pattern Templating.

Bulk fabrication of surface patterns with sub-20 nm feature sizes is immensely desirable for many existing and emerging technologies.Directed self-assembly (dsa) of block copolymers (bcps) has been a recently demonstrated approach to achieve such feature resolution over large-scale areas with minimal defect populations.However, much work remains to understand and optimize dsa methods in order to move this field forward.This paper presents large-scale numerical simulations of zone annealing and chemo-epitaxy processing of bcp films to achieve long-range orientational order.The simulations utilize a time-dependent ginzburg-landau model and parallel processing to elucidate relationships between the magnitude and velocity of a moving thermal gradient and the resulting bcp domain orientations and defect densities.Additional simulations have been conducted to study to what degree orientational order can be further improved by combining zone annealing and chemo-epitaxy techniques.It is found that these two dsa methods do synergistically enhance long-range order with a particular relationship between thermal gradient velocity and chemical template spacing.

Journal: Scientific reports (2017)
Publication types: Journal Article;Research Support, U.S. Gov't, Non-P.H.S.;Research Support, Non-U.S. Gov't
Clinical characterization of insomnia in adolescents - an integrated approach to psychopathology.

S/background insomnia in adolescence is common and debilitating yet it remains poorly understood.Here, we examine the complexity of clinical, behavioral, and psychosocial factors characterizing insomnia in adolescents.Methods ninety-five adolescents (16-19 years) with (n = 47, 31 female) and without (n = 48, 28 female) insomnia symptoms participated.In the insomnia group, 26 (20 female) met full dsm-5 criteria for insomnia disorder, while 21 (11 female) met partial criteria.Participants completed a clinical interview and assessments of clinical, behavioral, and psychosocial dimensions associated with insomnia.Glms and network analyses were used to evaluate group and sex differences in severity and inter-relationships between symptoms.Results adolescents with insomnia symptomatology had lower sleep hygiene and thought control, more depressive symptoms and dysfunctional sleep-related cognitions, and more substance use as a coping behavior than healthy controls.They also indicated higher neuroticism, stress levels, and sleep stress reactivity (p < 0.05), but no difference in adverse childhood experiences, than controls.Girls compared to boys with insomnia reported lower sleep quality, and more pre-sleep cognitive activity and sleep stress reactivity (p < 0.05).Compared to healthy girls, girls in the insomnia group reported lower sleep hygiene and higher agreeableness.Network analyses confirmed profound group differences in network topology, with the insomnia group having different levels of centrality and relationships between clinical characteristics compared with controls.Conclusions findings highlight clinical and sex-specific characteristics of adolescent insomnia, with network analyses revealing a complex interplay between clinical, behavioral, and psychosocial domains.Adolescents with insomnia symptoms, particularly girls, may benefit from interventions to improve negative cognition, mood, and stress, and behavioral strategies to counteract sleep-related maladaptive behaviors.

Journal: Sleep medicine (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural
Custom design and biomechanical analysis of 3D-printed PEEK rib prostheses.

A tumour resection normally involves a large tissue resection and bone replacement.Polyether ether ketone (peek) has become a suitable candidate for use in various prostheses owing to its lightness in weight, modulus close to that of natural bone, and good biocompatibility, among other factors.This study proposes a new design method for a rib prosthesis using the centroid trajectory of the natural replaced rib, where the strength can be adjusted by monitoring the cross-sectional area, shape, and properties.A custom-designed rib prosthesis was manufactured using fused deposition modelling (fdm) manufacturing technology, and the mechanical behaviour was found to be close to that of a natural rib.A finite element analysis of the designed rib was carried out under similar loading conditions to those used in mechanical testing.The results indicate that the centroid trajectory derived from a natural rib diaphysis can provide reliable guidance for the design of a rib prosthesis.Such methodology not only offers considerable design freedom in terms of shape and required strength, but also benefits the quality of the surface finishing for samples manufactured using the fdm technique.Fdm-printed peek rib prostheses have been successfully implanted, and good clinical performances have been achieved.

Journal: Biomechanics and modeling in mechanobiology (2018)
Publication types: Journal Article
Efficacy of Cinnamon as an Adjuvant in Reducing the Glycemic Biomarkers of Type 2 Diabetes Mellitus: A Three-Month, Randomized, Triple-Blind, Placebo-Controlled Clinical Trial.

The major aim of this randomized, placebo-controlled, triple-blind clinical trial was to evaluate the efficacy of cinnamon as an adjuvant treatment in reducing glycemic levels in people with type 2 diabetes, compared to a placebo.The study was conducted between august and december 2019, with 160 people with type 2 diabetes, in five primary health units, in parnaíba, brazil.Inclusion criteria were: persons of both genders using oral antidiabetic agents, with glycated hemoglobin ≥ 6.0%, and between 18 and 80 years of age.The primary outcome was change in glycated hemoglobin levels after 90 days of intervention.Other biomarkers evaluated were fasting blood glucose, insulin level, and homa-ir index.Participants were divided equally into two groups of 80 individuals each, and were given 3 g capsules of either cinnamon or placebo to be taken in combination with their usual oral antidiabetic agents.After 90 days, participants in the cinnamon group had statistically significant reductions of 0.2% of glycated hemoglobin and 0.55 mmol/l of fasting venous glucose, when compared with the placebo group.Cinnamon reduced the glycemic measures of persons with type 2 diabetes, albeit with modest reductions.Trial: rbr-2kkb6d.

Journal: Journal of the American Nutrition Association (2022)
Publication types: Journal Article;Randomized Controlled Trial;Research Support, Non-U.S. Gov't