syedzayyan/paper_mill_benchmark huggingface dataset:
Distinct TrkA and Ret modulated negative and positive neuropathic behaviors in a mouse model of resiniferatoxin-induced small fiber neuropathy.

Neurotrophic factors and their corresponding receptors play key roles in the maintenance of different phenotypic dorsal root ganglion (drg) neurons, the axons of which degenerate in small fiber neuropathy, leading to various neuropathic manifestations.Mechanisms underlying positive and negative symptoms of small fiber neuropathy have not been systematically explored.This study investigated the molecular basis of these seemingly paradoxical neuropathic behaviors according to the profiles of trk a and ret with immunohistochemical and pharmacological interventions in a mouse model of resiniferatoxin (rtx)-induced small fiber neuropathy.Mice with rtx neuropathy exhibited thermal hypoalgesia and mechanical allodynia, reduced skin innervation, and altered drg expression profiles with decreased trk a(+) neurons and increased ret(+) neurons.Rtx neuropathy induced the expression of activating transcription factor 3 (atf3), and atf3(+) neurons were colocalized with ret but not with trk a (p<0.001).As a neuroprotectant, 4-methylcatechol (4mc) promoted skin reinnervation partially with correlated reversal of the neuropathic behaviors and altered neurochemical expression.Gambogic amide, a selective trk a agonist, normalized thermal hypoalgesia, and gw441756, a trk a kinase inhibitor, induced thermal hypoalgesia, which was already reversed by 4mc.Mechanical allodynia was reversed by a ret kinase inhibitor, ast487, which induced thermal hyperalgesia in naïve mice.The activation of ret signaling by xib4035 induced mechanical allodynia and thermal hypoalgesia in rtx neuropathy mice in which the neuropathic behaviors were previously normalized by 4mc.Distinct neurotrophic factor receptors, trk a and ret, accounted for negative and positive neuropathic behaviors in rtx-induced small fiber neuropathy, respectively: trk a for thermal hypoalgesia and ret for mechanical allodynia and thermal hypoalgesia.

Journal: Experimental neurology (2018)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Prevalence and incidence of stroke, white matter hyperintensities, and silent brain infarcts in patients with chronic heart failure: A systematic review, meta-analysis, and meta-regression.

Introduction heart failure (hf) is associated with ischemic stroke (is).However, there are limited studies on the prevalence of is, white matter hyperintensities (wmhs), and silent brain infarcts (sbis).Furthermore, interaction with ejection fraction (ef) is unclear.Methods we searched three databases (viz., pub med, embase, and cochrane) for studies reporting the incidence or prevalence of is, wmhs, and sbis in hf.A total of two authors independently selected included studies.We used random-effects models, and heterogeneity was evaluated with i2statistic.Meta-regression was used for subgroup analysis.Results in total, 41 articles involving 870,002 patients were retrieved from 15,267 records.Among patients with hf, the pooled proportion of is was 4.06% (95% ci: 2.94-5.59), and that of wmhs and sbis was higher at 15.67% (95% ci: 4.11-44.63) and 23.45% (95% ci: 14.53-35.58), respectively.Subgroup analysis of hfp ef and hfr ef revealed a pooled prevalence of 2.97% (95% ci: 2.01-4.39) and 3.69% (95% ci: 2.34-5.77), respectively.Subgroup analysis of wmh fazekas scores 1, 2, and 3 revealed a decreasing trend from 60.57 % (95% ci: 35.13-81.33) to 11.57% (95% ci: 10.40-12.85) to 3.07% (95% ci: 0.95-9.47).The relative risk and hazard ratio of patients with hf developing is were 2.29 (95% ci: 1.43-3.68) and 1.63 (95% ci: 1.22-2.18), respectively.Meta-regression showed is prevalence was positively correlated with decreasing anticoagulant usage.Conclusion we obtained estimates for the prevalence of is, wmh, and sbi in hf from systematic review of the literature.Systematic review registration id=255126, prospero [crd42021255126].

Journal: Frontiers in cardiovascular medicine (2022)
Publication types: Systematic Review;Journal Article
Patients with Metastatic Colorectal Cancer after Failure of Second-Line Treatment May Benefit from Low-Dose Apatinib and S-1 Combined with Jianpi Bushen Jiedu Decoction.

To evaluate the effect and safety of low-dose of apatinib and s-1 combined with jianpi bushen jiedu decoction (jbjd) in patients with metastatic colorectal cancer (m crc) who have failed second or above lines treatment, in order to provide more treatment option for m crc patients by integrated medicine.Methods thirteen patients were selected from a single-arm, open-label clinical study from april 2019 to september 2020.The patients were treated with low-dose apatinib (250 mg, once a day) and s-1 (20 mg, twice a day) combined with jbjd for at least one cycle and were followed up to august 2021.The primary endpoint was disease progression-free survival (pfs).Disease control rate (dcr), objective response rate (orr), and overall survival (os) of patients were observed as the secondary endpoints.Adverse events were recorded as well.Results the average age of the 13 patients was 56.5 ±13.0 years and 76.9% were male.The median pfs and median os were 4.6 and 8.3 months, respectively.The orr was 7.7% (1/13) while the dcr was 61.5% (8/13).The common adverse events were hypertension, proteinuria, elevated transaminase, and thrombocytopenia.One patient experienced thrombocytopenia of grade 3.Conclusions patients with m crc after failure of the second or above lines of treatment may potentially benefit from the treatment of low-dose apatinib and s-1 combined with jbjd because of its similar effect as the standard dose of target therapy and relatively better safety.(registration no.Chi ctr1900022673).

Journal: Chinese journal of integrative medicine (2022)
Publication types: Clinical Trial;Journal Article
Cellulose-based hydrogel on quantum dots with molecularly imprinted polymers for the detection of CA19-9 protein cancer biomarker.

Molecularly imprinted polymers mips were successfully assembled around quantum dots (qds), for the detection of the protein biomarker ca19-9 associated to pancreatic cancer (pc).These imprinted materials mip@qds were incorporated within the cellulose hydrogel with retention of its conformational structure inside the binding cavities.The concept is to use mips which function as the biorecognition elements, conjugated to cadmium telluride qds as the sensing system.The excitation wavelength was set to 477 nm and the fluorescence signal was measured at its maximum intensity, with an emission range between 530 and 780 nm.The fluorescence quenching of the imprinted cellulose hydrogels occurred with increasing concentrations of ca19-9, showing linearity in the range 2.76 × 10-2- 5.23 × 102u/ml, in a 1000-fold diluted human serum.Replicates of the imprinted hydrogel show a linear response below the cut-off values for pancreatic cancer diagnosis (< 23 u/ml), a limit of detection of 1.58 × 10-3u/ml and an imprinting factor (if) of 1.76.In addition to the fact that the imprinted cellulose hydrogel displays good stability and selectivity towards ca19-9 when compared with the non-imprinted controls, the conjugation of mips to qds increases the sensitivity of the system for an optical detection method towards ranges within clinical significance.This fact shows potential for the imprinted hydrogel to be applied as a sensitive, low-cost format for point-of-care tests (po cts).

Journal: Mikrochimica acta (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Evaluation of the therapeutic effect of mesoporous silica nanoparticles loaded with Gallic acid on reserpine-induced depression in Wistar rats.

The current study evaluates the free gallic acid (ga) and ga-loaded mesoporous silica nanoparticles (msns) antidepressant efficacy in a rat model of depression caused by reserpine.Methods by using a scanning electron microscope (sem), dynamic light scattering (dls), and zeta potential, msns and ga-loaded msns were characterized.The efficiency of encapsulation and the release of ga-loaded msns were also investigated.The effect of ga, either in its free form or loaded on (msns) on oxidative stress biomarkers and monoamine neurotransmitters levels (serotonin (5-ht), norepinephrine (nep), and dopamine (da)), were evaluated in these areas (cortex, hippocampus, striatum, and hypothalamus) of control, a depression model of rat, a depression model of rat treated with either free ga, msns or ga loaded msns.The forced swimming test (fst) also the open field test (oft) were carried out to evaluate the behavioral changes in all groups.Results reserpine caused a decrease in the time spent in motor and swimming activity besides increasing the time of immobility, as demonstrated by oft and fst.Significantly reductions in 5-ht, nep, and da were obtained in the cortex, hippocampus, hypothalamus, and striatum of reserpine-treated rats.Free ga was more effective in increasing the serotonin level in the cortex, hippocampus, and hypothalamus, while ga-loaded msns were more effective in increasing it in the striatum.Ga-loaded msns also increased the level of nep in the four studied brain areas.Free ga increased dopamine levels in the cortex and striatum, whereas ga-loaded msns increased da levels in the hippocampus and hypothalamus compared with the depressed untreated group.Conclusions msns can be used as a drug delivery system to target ga selectively to specific brain areas.

Journal: BMC pharmacology & toxicology (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Secretion of equine chorionic gonadotropin and its association with supplementary corpus luteum formation and progesterone concentration in Hokkaido native pony recipient mares.

The objectives of this study were to determine the plasma profile of equine chorionic gonadotropin (e cg) and its association with the formation of supplementary corpus luteum (cl) and plasma progesterone concentrations in embryo transfer hokkaido native pony recipient mares.Blood samples and transrectal ultrasound examination of the reproductive tract were carried out weekly from the day of ovulation until week 32 of gestation (n = 4).Plasma concentrations of e cg and progesterone were measured by enzyme immunoassays.The e cg concentration was first detectable at week 5 for 2 mares and at week 6 for another 2 mares.Immediately after detection, the mean plasma e cg concentrations were observed to rise sharply and reach a peak at week 8.The concentrations then declined dramatically to a baseline (<0.5 iu/m l) by week 21.Plasma progesterone p=p concentrations increased in 2 phases.First, a sharp increase from 0.18 ± 0.05 ng/m l at ovulation to 15.9 ± 4.6 ng/m l at week 1 was observed, then a decrease to 9.69 ± 2.27 ng/m l by week 2, and maintained at this level until week 5 of gestation.The onset of the second rise occurred at week 6 and was observed to peak to 58.3 ± 21.8 ng/m l at week 10, then gradually declined to <10 ng/m l by week 26.The supplementary cls were first detectable by pregnancy week 6 and 7 for 2 mares each.All supplementary and primary cls regressed by week 26 for 3 mares and by week 30 for the remaining mare.The mean number of supplementary cl was 4.5 ± 0.8 and their formation in the right ovary (66.7%, 12/18) was higher (p < 0.05) than that in the left ovary (33.3%, 6/18).Among the mares, 1 mare that developed only 2 supplementary cl had 35% lower level of peak e cg and 65% lower concentration of peak progesterone compared with other 3 mares that had 5 or 6 supplementary cl.In conclusion, development of supplementary cl and blood concentrations of progesterone from around day 40 of gestation were associated with e cg concentration.The total number of supplementary cl formation in the present study in embryo transfer hokkaido native pony recipient mares seemed higher than previously reported supplementary cl number in pregnant mares, with a greater rate in the right ovary than in left.

Journal: Domestic animal endocrinology (2020)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Unpredictability of maternal environment shapes offspring behaviour without affecting stress-induced cortisol in an annual vertebrate.

Exposure of females to stressful conditions during pregnancy or oogenesis has a profound effect on the phenotype of their offspring.For example, offspring behavioural phenotype may show altered patterns in terms of the consistency of behavioural patterns and their average level of performance.Maternal stress can also affect the development of the stress axis in offspring leading to alterations in their physiological stress response.However, the majority of evidence comes from studies utilising acute stressors or exogenous glucocorticoids, and little is known about the effect of chronic maternal stress, particularly in the context of stress lasting throughout entire reproductive lifespan.To bridge this knowledge gap, we exposed female sticklebacks to stressful and unpredictable environmental conditions throughout the breeding season.We quantified the activity, sheltering and anxiety-like behaviour of offspring from three successive clutches of these females, and calculated intra-class correlation coefficients for these behaviours in siblings and half-siblings.We also exposed offspring to an acute stressor and measured their peak cortisol levels.An unpredictable maternal environment had no modifying effect on inter-clutch acute stress responsivity, but resulted in diversification of offspring behaviour, indicated by an increased between-individual variability within families.This may represent a bet-hedging strategy, whereby females produce offspring differing in behavioural phenotype, to increase the chance that some of these offspring will be better at coping with the anticipated conditions.

Journal: Hormones and behavior (2023)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Predictors of selection into an elite level youth football academy: A longitudinal study.

Talent identification in football is complex.Research regarding talent indicators that predict selection into professional academies is increasingly multi-disciplinary, though is typically cross-sectional, failing to consider whether therate of changeof those indicators influences selection.The aim of this study was to determine if longitudinal changes in talent indicators are associated with selection into a professional football academy.A total of 110 dutch male football players (aged 8-12) playing in a youth development programme of a professional club were included in the study.Players were assessed regularly on their anthropometry, physical fitness, gross motor coordination, technical ability, and psychosocial capacities over four years.A subset of players was then selected into the academy.Cross-sectional and longitudinal analyses revealed the indicators that best predicted selection.The best indicator was the 30 m sprint speed, with prediction not improving when including other predictors or their rate of change.The individuals that the club ultimately selected at age 12 could have been predicted well above chance levels using the sprint speeds at age nine or ten.The relative consistency of the rate of improvement in indicators across participants meant that the rate at which they developed played little role in selections.

Journal: Journal of sports sciences (2022)
Publication types: Journal Article
Adenocarcinoma and Neuroendocrine Collision Tumor in a Giant Gastric Hyperplastic Polyp.

Screening esophagogastroduodenoscopy of a 65-year-old man revealed a 4.7-cm polypoid in the gastric high body.Clinical and laboratory findings, including serum gastrin level (460 pg/m l) and biopsy findings, were consistent with a diagnosis of type i neuroendocrine tumor (net).Histologically, the mass consisted of dilated tortuous glands at the surface and grade 1 net in deeper tissue.Some hyperplastic glands exhibited a transition to adenocarcinoma, which invaded the net, simulating a "tumor in tumor" appearance.Next-generation sequencing revealed that the adenocarcinoma component harbored a tp53 mutation, whereas the net component showed no pathogenic mutation.To our knowledge, this unusual collision of adenocarcinoma and net within a single gastric hyperplastic polyp has not been previously described.This case suggests that large gastric hyperplastic polyps should be carefully examined because of the possibility of underlying net and malignant transformation of surface epithelium.

Journal: Journal of Nippon Medical School = Nippon Ika Daigaku zasshi (2020)
Publication types: Case Reports;Journal Article;Review
A novel approach for predicting acute hospitalizations among elderly recipients of home care? A model development study.

Frail elderly are at high risk of hospitalizations and have a complex pattern of risk factors that makes it hard to foresee potential needs for additional treatment and care.Machine learning algorithms are potentially well-suited to discover hidden patterns in registrations that are routinely made across sectors.Objective to investigate predictors and performance of machine learning algorithms designed to predict acute hospitalizations in elderly recipients of home care services.Materials and methods a development study based on a retrospective social sector cohort with 1,282 participants was designed.Included subjects were aged 65 or older and received home care services in aalborg municipality at least once a week from 1/1-2016 to 31/12-2017.Data were collected from a newly developed triage tool in combination with administrative and clinical data routinely collected in the danish healthcare and social care sector.857 predictors were tested and evaluated based on the area under the precision-recall curve (pr-auc).The data was divided into a 70/30 training and test split with 5-fold cross-validation.A sliding window approach combining random under-sampling with a boosting algorithm (rusboost) was applied with a standard logistic regression included for comparison.Results the logistic regression achieved a pr-auc of 0.01 (ci 0.00; 0.01) while the pr-auc was 0.71 (ci 0.56; 0.76) for the rusboost algorithm.Four of the five most important citizen-level features used to accurately predict an acute hospitalization was the total number of services provided by the municipality to the citizen, the number of personal care registrations as well as number of medication handlings and nutritional registrations.A final important predictor was the number of physical complaints derived from the triage tool.Conclusions municipalities routinely collect valuable administrative and clinical data that can be used for early prediction of acute hospitalizations.However, future studies are needed to validate the results.

Journal: International journal of medical informatics (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
A Case of Atypical Bartonellosis in a 4-Year-Old Immunocompetent Child.

In most cases, infection due to bartonella henselaecauses a mild disease presenting with a regional lymphadenopathy frequently associated with a low-grade fever, headache, poor appetite and exhaustion that spontaneously resolves itself in a few weeks.As the infection is generally transmitted by cats through scratching or biting, the disease is named cat scratch disease (csd).However, in 5-20% of cases, mainly in immunocompromised patients, systemic involvement can occur and csd may result in major illness.This report describes a case of systemic csd diagnosed in an immunocompetent 4-year-old child that can be used as an example of the problems that pediatricians must solve to reach a diagnosis of atypical csd.Despite the child's lack of history suggesting any contact with cats and the absence of regional lymphadenopathy, the presence of a high fever, deterioration of their general condition, increased inflammatory biomarkers, hepatosplenic lesions (i.e., multiple abscesses), pericardial effusion with mild mitral valve regurgitation and a mild dilatation of the proximal and medial portion of the right coronary artery, seroconversion for b.Henselae(ig g 1:256) supported the diagnosis of atypical csd.Administration of oral azithromycin was initiated (10 mg/kg/die for 3 days) with a progressive normalization of clinical, laboratory and us hepatosplenic and cardiac findings.This case shows that the diagnosis of atypical csd is challenging.The nonspecific, composite and variable clinical features of this disease require a careful evaluation in order to achieve a precise diagnosis and to avoid both a delayed diagnosis and therapy with a risk of negative evolution.

Journal: Microorganisms (2021)
Publication types: Case Reports;Journal Article
Toolboxes for plant systems biology research.

The terms 'systems' and 'synthetic biology' are often used together, with most scientists striding between the two fields rather than adhering to a single side.Often too, scientists want to understand a system to inform the design of gene circuits that could endow it with new functions.However, this does not need to be the progression of research, as synthetic constructs can help improve our understanding of a system.Here, we review synthetic biology tool kits with the potential to overcome pleiotropic effects, compensatory mechanisms, and redundancy in plants.Combined with -omics techniques, these tools could reveal novel insights on plant growth and development, an aim that has gained renewed urgency given the impact of climate change on crop productivity.

Journal: Current opinion in biotechnology (2022)
Publication types: Journal Article;Review;Research Support, U.S. Gov't, Non-P.H.S.;Research Support, Non-U.S. Gov't
Potential Application of Alternative Materials for Organic Pollutant Removal.

The work aims to synthesize and characterize vegetal charcoal (or biochar) from syzygium cumini (ac-sc), evaluating the adsorption capacity for dexamethasone drug (dex) removal, using the kinetic and equilibrium adsorption.The samples were characterized by n2porosimetry, x-ray diffraction, scanning electron microscopy with energy-dispersive spectroscopy, zeta potential, and zero charge point.Adsorption equilibrium was carried out applying the langmuir, freundlich, redlich-peterson, sips, and toth models, and kinetic adsorption applied the pseudo-first order, pseudo-second order, elovich, avrami, and weber-morris models.Ac-sc showed a heterogeneous and porous surface, negatively charged, crystalline structure, specific surface area of the 2.14 m2g-1and p hzcp= 7.36.About the effect of the ac-sc concentration, 5.0 g l-1showed the best dex removal (53.02%), about the others' concentration (2.0 and 7.5 g l-1).About the equilibrium and kinetic adsorption, the sips model and pseudo-second order showed the best experimental data adjusted, indicating that the adsorption monolayer was dependent on the ions onto the biosorbent, with a maximum adsorption capacity of 0.744 mg g-1after 180 min.Therefore, ac-sc can be used as an alternative material in the removal of organic pollutants, such as drug removal.

Journal: Water, air, and soil pollution (2022)
Publication types: Journal Article