syedzayyan/paper_mill_benchmark huggingface dataset:
SPOCK2 Promotes the Malignant Behavior of Ovarian Cancer via Regulation of the Wnt/β-Catenin Signaling Pathway

Ovarian cancer (oc) is a common clinical gynecological disease, which seriously threatens women’s health and life.We investigated the roles of spock2 in oc and its associated molecular mechanism in the current study.Methods .The expressions and prognostic value of spock2 in oc were identified using the clinical data and data from the gepia database.Then, spock2 silence was implemented to search functions of spock2 in oc cells.Cck‐8 was used to examine cell proliferation.Cell apoptosis was detected by flow cytometry.The oc cell invasion and migration were evaluated by transwell assays.Results .Overexpressed spock2 was identified in oc, which was correlated with poor prognosis and a shorter survival rate.Spock2 downregulation significantly suppressed oc cell proliferation, migration, and invasion, and cell apoptosis was markedly promoted by spock2 silence.Meanwhile, spock2 knockdown could effectively suppress wnt/ β ‐catenin.Conclusion .Spock2 exerted crucial functions in oc progression and could serve as a promising candidate for oc targeted therapy.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Sirtuin 1 Induces Choroidal Neovascularization and Triggers Age-Related Macular Degeneration by Promoting LCN2 through SOX9 Deacetylation

Increasing studies have identified the function of sirtuin-1 (sirt1) in ocular diseases.Hence, this study is aimed at exploring the potential role of sirt1 in choroidal neovascularization- (cnv-) induced age-related macular degeneration (amd) development and the associated mechanism.Expression of sirt1/sox9/lcn2 in the hypoxic cells was determined, and their interactions were predicted by bioinformatics websites and followed by the verification by luciferase assay and chromatin immunoprecipitation (ch ip).Their in vitro effects on hypoxic cells concerning cell viability, apoptosis, migration, and angiogenesis were detected through gain- and loss-of-function assays.Besides, their in vivo effect was explored using the established cnv mouse models.Highly expressed lcn2, sox9, and sirt1 were observed in hypoxic cells.Lcn2 was increased by sox9 and sirt1 deacetylated sox9 to promote its nuclear translocation, which further inhibited the viability of human retinal pigment epithelial cells and promoted cell apoptosis and angiogenesis as well as cnv-induced amd formation.The relieving role of lcn2 inhibition on cnv-induced amd without toxicity for mice was also demonstrated by in vivo experiments.Overall, sirt1 promoted the formation of cnv-induced amd through sox9 deacetylation-caused lcn2 upregulation, representing a promising target for cnv-induced amd management.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Serum Metabolomics of Benign Essential Blepharospasm Using Liquid Chromatography and Orbitrap Mass Spectrometry

Benign essential blepharospasm (beb) is a form of focal dystonia that causes excessive involuntary spasms of the eyelids.Currently, the pathogenesis of beb remains unclear.This study is aimed at investigating the serum metabolites profiles in patients with beb and healthy control and to identify the mechanism and biomarkers of this disease.Methods.30 patients with beb and 33 healthy controls were recruited for this study.We conducted the quantitative and nontargeted metabolomics analysis of the serum samples from 63 subjects by using liquid chromatography and orbitrap mass spectrometry (lc-orbitrap ms).Multivariate statistical analysis was performed to detect and identify different metabolites between the two groups.The kyoto encyclopedia of genes and genomes (kegg) and receiver operating characteristic (roc) curve analysis of the altered metabolites were performed.Results.A total of 134 metabolites were found and identified.The metabolites belonged to several metabolic pathways including phenylalanine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, arginine biosynthesis, linoleic acid metabolism, tryptophan metabolism, aminoacyl-t rna biosynthesis, sphingolipid metabolism, glycosphingolipid biosynthesis, leucine and isoleucine biosynthesis, and vitamin b6 metabolism.Eight metabolites were identified as the potential biomarkers.Conclusions.These results demonstrated that serum metabolic profiling of beb patients was significantly different from healthy controls based on lc-orbitrap ms.Besides, metabolomics might provide useful information for a better understanding of beb.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Serum Markers CA125, CA153, and CEA along with Inflammatory Cytokines in the Early Detection of Lung Cancer in High-Risk Populations

Lung cancer mortality and morbidity rates are the first among malignant tumors.It is extremely crucial to pay more attention to the early diagnosis and treatment of lung cancer and to grasp and judge the progress of the patient’s condition promptly.In this study, lung cancer patients’ early diagnosis with tumor markers and inflammatory variables is examined.The general surgery department of our hospital treated 98 patients with lung cancer and 100 patients with benign pulmonary hyperplasia from january 2017 to february 2018.Additionally, 100 healthy subjects who completed physical examinations during this time period were included.Based on the findings of the pathological examination, 100 patients with benign pulmonary hyperplasia were chosen as the benign group, 100 patients with lung cancer were chosen as the malignant group, and 100 healthy individuals were chosen as the healthy group.The tumor markers carbohydrate antigen 125 (ca125), ca153, and carcinoembryonic antigen (cea), as well as inflammatory factors such as tumor necrosis factor‐ (tnf‐) and high‐sensitivity c‐reactive protein, were measured in the venous blood of three groups of patients (hs‐crp).There was no discernible difference in tumor marker levels between the benign and healthy groups ( p &gt; 0.05).In comparison to the benign and healthy groups, the malignant group had higher serum levels of ca153, ca125, and cea ( p 0.05).Between the benign and healthy groups, there was no discernible difference in the levels of inflammatory factors ( p &gt; 0.05).Tnf‐ and hs‐crp serum levels were observed to be higher in the malignant group compared to the benign and healthy groups ( p 0.05).The combined detection of ca153 + ca125 + cea + tnf − +hs − crp showed the highest sensitivity and specificity, which were, respectively, 62.22 percent and 92.00 percent, when compared to single or mixed detection of tumor markers or inflammatory factors solely.Serum levels of inflammatory agents tnf‐ and hs‐crp may be related to the pathophysiology and other functions of patients with lung cancer, as well as to the development and metastasis of the disease.These markers include ca153, ca125, and cea.For the early detection of lung cancer and the evaluation of the disease’s severity, the detection of tumor markers in combination with inflammatory variables has a significant reference value.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Serum Exosomal MicroRNAs Predict Acute Respiratory Distress Syndrome Events in Patients with Severe Community-Acquired Pneumonia

Severe community-acquired pneumonia (scap) requiring intensive care unit (icu) treatment commonly causes acute respiratory distress syndrome (ards) with high mortality.This study was aimed at evaluating whether micro rnas (mi rnas) in circulating exosomes have the predictive values for patients at risk of developing ards due to scap.Methods .Ards/ali-relevant mi rnas were obtained by literature search.Exosomes in serum were isolated by ultracentrifugation method and identified by transmission electron microscopy.Then the mi r profiling in the exosomes using real-time pcr was analyzed in scap patients with or without ards.Moreover, multivariate cox proportional regression analysis was performed to estimate the odds ratio of mi rna for the occurrence of ards and prognosis.The receiver operating characteristics (roc) curves were calculated to discriminate ards cases.Finally, the kaplan-meier curve using log-rank method was performed to test the equality for survival distributions with different mi rna classifiers.Results .A total of 53 scap patients were finally recruited.Ten mi rnas were picked out.Further, a subset of exosomal mi rnas, including the mi r-146a, mi r-27a, mi r-126, and mi r-155 in ards group exhibited significantly elevated levels than those in non-ards group.The combined expression of mi r-126, mi r-27a, mi r-146a, and mi r-155 predicted ards with an area under the curve of 0.909 (95 % ci 0.815 –1).Only mi r-126 was selected to have potential to predict the 28-day mortality (or=1.002, p=0.024) with its median value classifier.Conclusions .The altered levels of circulating exosomal micro rnas may be useful biologic confirmation of ards in patients with scap.

Journal: BioMed Research International (2019)
Publication types: Research Article;
Screening and Identification of Antidepressant Active Ingredients from Puerariae Radix Extract and Study on Its Mechanism

Depression is a common mental disease with long course and high recurrence rate.Previous studies showed that puerariae radix and its extracts have powerful antidepressant effects in recent years.The study proposed an integrated strategy, combining network pharmacology and molecular pharmacology experiment to investigate the mechanisms of the antidepressant active ingredients from puerariae radix.Methods .Tcmsp database, gene cards database, venny 2.1, uni prot database, string database, cytoscape 3.7.2, and metascape database were used to screen the active chemical components, antidepressant‐related genes, and core targets, convert the abbreviated gene names in batch, search and predict the interaction between proteins, and construct the ppi network of puerariae radix .Kegg pathway and go biological process enrichment and biological annotation were used to select antidepressant core gene targets.The mtt method was used to detect the effect of puerarin on the damage of pc12 cells induced by corticosterone.The dcfh‐da probe and ros assay kit were utilized to detect the production of ros in pc12 cells.Pi/annexin v was used to detect the apoptotic rate of puerarin on pc12 cells.Western blotting was used to verify the regulation of puerarin on the key targets of akt1, fos, casp3, stat3, and tnf‐ α in pc12 cells.Results and conclusion .Eight main active components, 64 potential antidepressant gene targets, and 15 core antidepressant gene targets were obtained.35 signaling pathways and 52 biological processes related to antidepressant effect of puerariae radix were identified.Puerarin was the active ingredient derived from puerariae radix which exhibited the antidepression effect by improving the viability of cell, reducing cell apoptosis, regulating ros production, increasing protein expressions of akt1 and fos, and reducing protein expressions of casp3, stat3, and tnf‐ α .The study revealed the pharmacodynamic material basis and possible antidepressant mechanism of puerariae radix and provided new theoretical basis and ideas for antidepressant research.

Journal: Oxidative Medicine and Cellular Longevity (2021)
Publication types: Research Article;
ROS Signaling-Mediated Novel Biological Targets: Brf1 and RNA Pol III Genes

Biomolecule metabolism produces ros (reactive oxygen species) under physiological and pathophysiological conditions.Dietary factors (alcohol) and carcinogens (egf, den, and mnng) also induce the release of ros.Ros often causes cell stress and tissue injury, eventually resulting in disorders or diseases of the body through different signaling pathways.Normal metabolism of protein is critically important to maintain cellular function and body health.Brf1 (transcript factor ii b‐related factor 1) and its target genes, rna pol iii genes (rna polymerase iii‐dependent genes), control the process of protein synthesis.Studies have demonstrated that the deregulation of brf1 and its target genes is tightly linked to cell proliferation, cell transformation, tumor development, and human cancers, while alcohol, egf, den, and mnng are able to induce the deregulation of these genes through different signaling pathways.Therefore, it is very important to emphasize the roles of these signaling events mediating the processes of brf1 and rna pol iii gene transcription.In the present paper, we mainly summarize our studies on signaling events which mediate the deregulation of these genes in the past dozen years.These studies indicate that brf1 and rna pol iii genes are novel biological targets of ros.

Journal: Oxidative Medicine and Cellular Longevity (2021)
Publication types: Review Article;
Role of Glial Cell-Derived Oxidative Stress in Blood-Brain Barrier Damage after Acute Ischemic Stroke

The integrity of the blood-brain barrier (bbb) is mainly maintained by endothelial cells and basement membrane and could be regulated by pericytes, neurons, and glial cells including astrocytes, microglia, oligodendrocytes (ols), and oligodendrocyte progenitor cells (opcs).Bbb damage is the main pathological basis of hemorrhage transformation (ht) and vasogenic edema after stroke.In addition, bbb damage-induced ht and vasogenic edema will aggravate the secondary brain tissue damage.Of note, after reperfusion, oxidative stress-initiated cascade plays a critical role in the bbb damage after acute ischemic stroke (ais).Although endothelial cells are the target of oxidative stress, the role of glial cell-derived oxidative stress in bbb damage after ais also should receive more attention.In the current review, we first introduce the physiology and pathophysiology of the bbb, then we summarize the possible mechanisms related to bbb damage after ais.We aim to characterize the role of glial cell-derived oxidative stress in bbb damage after ais and discuss the role of oxidative stress in astrocytes, microglia cells and oligodendrocytes in after ais, respectively.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Role of 5-Hydroxytryptamine and Intestinal Flora on Depressive-Like Behavior Induced by Lead Exposure in Rats

To investigate the effects of 5‐hydroxytryptamine (5‐ht) and intestinal flora on depression‐like behavior induced by lead exposure in rats.Methods .30 healthy spf adult male sd rats were randomly divided into control group and lead exposure group.The depression‐like behavior of rats was detected.The blood, striatum, and intestinal tissue were collected.The lead content was detected by icp‐ms.The m rna expressions of chg a, tph1, 5‐ht, and 5‐ht3r were tested by q rt‐pcr.The content of 5ht was checked by hplc‐ecd.The content of 5‐ht3r was detected by elisa.The protein expressions of 5‐ht, 5‐ht3r, chg a, and tph were gauged by immunohistochemistry.Fecal samples were collected, and the composition of intestinal flora in experimental rats was analyzed by 16 s rna metagene sequencing.Results .Lead exposure can greatly cause depression.The content of 5‐ht in blood and striatum in the lead exposure group decreased, and the expression levels of 5‐ht, 5‐ht3 r, chg a, and tph in the intestine decreased distinctly.Compared with the control group, the distribution of a‐polymorphism related indexes simpson, chao1, shannon, and ace in rats with depressive‐like behavior after lead exposure was significantly increased; in the lead exposure group, there were 61 different operational taxonomic units (outs) in intestinal flora at the family level.Based on linear discriminant analysis, it was found that the key bacteria were lactobacillaceae and bifidobacteriaceae, and their abundance decreased evidently in the lead exposure group.Conclusion .Lead exposure improves depressive‐like behavior by affecting intestinal flora and regulating neurotransmitter 5‐ht through the intestinal‐brain axis.

Journal: BioMed Research International (2021)
Publication types: Research Article;
Rocaglamide Prolonged Allograft Survival by Inhibiting Differentiation of Th1/Th17 Cells in Cardiac Transplantation

Aglaia (meliaceae) species are used for treating autoimmune disorders and allergic diseases in asian countries.Rocaglamide, an extract obtained from aglaia species, exhibits suppressive effect by regulating the t cell subset balance and cytokine network in cancer.However, whether it can be used in organ transplantation is unknown.In this study, we investigated the antirejection effect and mechanism of action of rocaglamide in a mouse cardiac allograft model.Methods.Survival studies were performed by administering mice with phosphate-buffered saline (pbs) ( n = 6 ) and rocaglamide ( n = 8 ).Heart grafts were monitored until they stopped beating.After grafting, the mice were sacrificed on day 7 for histological, mixed lymphocyte reaction (mlr), enzyme-linked immunosorbent assay (elisa), and flow cytometric analyses.Results.Rocaglamide administration significantly prolonged the median survival of the grafts from 7 to 25 days compared with pbs treatment ( p &lt; 0.001 ).On posttransplantation day 7, the rocaglamide-treated group showed a significant decrease in the percentage of th1 cells ( 7.9 ± 0.9 % vs.1.58 ± 0.5 % , p &lt; 0.001 ) in the lymph nodes and spleen ( 8.0 ± 2.5 % vs.2.4 ± 1.3 % , p &lt; 0.05 ).Rocaglamide treatment also significantly inhibited the production of th17 cells ( 6.4 ± 1.0 % vs.1.8 ± 0.4 % , p &lt; 0.01 ) in the lymph nodes and spleen ( 5.9 ± 0.3 % vs.2.9 ± 0.8 % , p &lt; 0.01 ).Furthermore, the prolonged survival of the grafts was associated with a significant decrease in ifn-γ and il-17 levels.Our results also showed that nf-at activation was inhibited by rocaglamide, which also induced p38 and jun n-terminal kinase (jnk) phosphorylation in jurkat t cells.Furthermore, by using inhibitors that suppressed p38 and jnk phosphorylation, rocaglamide-mediated reduction in nf-at protein levels was prevented.Conclusion.We identified a new immunoregulatory property of rocaglamide, wherein it was found to regulate oxidative stress response and reduce inflammatory cell infiltration and organ injury, which have been associated with the inhibition of nf-at activation in t cells.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Robust Zero Watermarking Algorithm for Medical Images Based on Zernike-DCT

Digital medical system not only facilitates the storage and transmission of medical information but also brings information security problems.Aiming at the security of medical images, a robust zero watermarking algorithm for medical images based on zernike-dct is proposed.The algorithm first uses a chaotic logic sequence to preprocess and encrypt the watermark, then performs edge detection and zernike moment processing on the original medical image to get the accurate edge points, and then performs discrete cosine transform (dct) on them to get the feature vector.Finally, it combines perceptual hash and zero watermark technology to generate the key to complete the watermark embedding and extraction.The algorithm has good robustness to conventional and geometric attacks, strong antinoise ability, high positioning accuracy, and processing efficiency and is superior to the classical edge detection algorithm in extraction effect.It is a stable and reliable image edge detection algorithm.

Journal: Security and Communication Networks (2021)
Publication types: Research Article;
Response Surface Methodology Approach to Predict the Flexural Moment of Ferrocement Composites with Weld Mesh and Steel Slag as Partial Replacement for Fine Aggregate

Design of experiment-response surface methodology approach is adopted to obtain the optimal flexural moment of ferrocement composites comprising galvanised square weld mesh with weight fraction of fine aggregate by steel slag.To get the optimal combination of progression variables on a flexural moment of ferrocement composites, the central composite design of response surface methodology was adopted.Regression models for responses were justified using analysis of variance and the pareto chart.The test results show that a maximum ultimate load of 3.30 k n and moment capacity of 220 k nm was obtained for ferrocement with a volume fraction of 2.733% and steel slag of 25% replacement.From the analysis of variance, it is evident that the p value is less than 0.005, the predicted r2 and the adjustable r2 are less than 20%, and the predicted values go in hand with the experimental result which indicates that the proposed models are highly suitable.Moreover, the volume fraction of galvanised square weld mesh has a higher significance on a flexural moment of ferrocement composites.Surface plot, pareto chart, and regression analysis outcomes show that the most substantial and influential factor for a flexural moment is the volume fraction of galvanised square weld mesh.

Journal: Advances in Materials Science and Engineering (2022)
Publication types: Research Article;
Robust and Secure Zero-Watermarking Algorithm for Medical Images Based on Harris-SURF-DCT and Chaotic Map

To protect the patient information in medical images, this article proposes a robust watermarking algorithm for medical images based on harris-surf-dct.First, the corners of the medical image are extracted using the harris corner detection algorithm, and then, the previously extracted corners are described using the method of describing feature points in the surf algorithm to generate the feature descriptor matrix.Then, the feature descriptor matrix is processed through the perceptual hash algorithm to obtain the feature vector of the medical image, which is a binary feature vector with a size of 32 bits.Secondly, to enhance the security of the watermark information, the logistic map algorithm is used to encrypt the watermark before embedding the watermark.Finally, with the help of cryptography knowledge, third party, and zero-watermarking technology, the algorithm can embed the watermark without modifying the medical image.When extracting the watermark, the algorithm can extract the watermark from the test image without the original image.In addition, the algorithm has strong robustness to conventional attacks and geometric attacks.Especially under geometric attacks, the algorithm performs better.

Journal: Security and Communication Networks (2021)
Publication types: Research Article;