syedzayyan/paper_mill_benchmark huggingface dataset:
Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosome Repairs Endometrial Epithelial Cells Injury Induced by Hypoxia via Regulating miR-663a/CDKN2A Axis

Thin endometrium remains a severe clinical challenge with no effective therapy to date.We aimed at exploring the role and molecular mechanism of human umbilical cord mesenchymal stem cell- (huc msc-) derived exosomes (huc msc-ex) in repairing hypoxic injury of endometrial epithelial cells (eecs).Methods.Exosomes were harvested from the conditioned medium of huc msc and characterized using western blot, transmission electron microscopy (tem), flow cytometry, and nanoparticle tracking analysis (nta).Eecs were subjected to hypoxic conditions before cocultured with huc msc-ex.Cell viability, apoptosis, and migration were determined with cck-8, flow cytometry, and wound healing assay, respectively.Apoptosis/emt-related proteins were detected by western blot.The mi rna profiling was determined by rna sequencing.The expression of mi r-663a and cdkn2a was measured by q rt-pcr.Mi r-663a in eecs was overexpressed by transfecting with mi r-663a mimics.Results.Mesenchymal stem cells (mscs) markers cd73, cd90, and cd106 were positively expressed in huc mscs.Exosome isolated from huc msc expressed cd63 and tsg101, and were 100–150 nm in diameter.Huc msc-ex promoted cell proliferation inhibited by hypoxia.And huc msc-ex also inhibited hypoxia-induced apoptosis, migration, and emt of eecs by upregulating the expression of bcl-2 and e-cadherin and downregulating bax and n-cadherin levels.Further, bioinformatics research found that huc msc-ex coculture can significantly upregulate the expression of mi r-663a and decrease the expression of cdkn2a in hypoxia-induced eecs.Furthermore, mi r-663a overexpression inhibited cdkn2a expression and increased the expression of bcl-2 and e-cadherin in hypoxia-induced eecs.Conclusions.Huc msc-ex promoted cell proliferation, inhibited cell apoptosis, migration, and emt in hypoxia-induced eecs, thereby alleviating hypoxia-induced eecs injury, which may be related to its regulation of mi r-663a/cdkn2a expression.Our study indicated that huc msc-ex might benefit for repairing thin endometrium.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Hsp72-Based Effect and Mechanism of Microwave Radiation-Induced Cardiac Injury in Rats

The purpose of this study was to determine the role of heat shock protein 72 (hsp72) changes in cardiac injury caused by microwave radiation, aimed at providing novel insights into the mechanism of this damage.A digital thermometer was used to measure the rectal temperature of the rats’ pre- and post-radiation.On the 1st, 7th, 14th, and 28th days post-radiation, the changes in electrocardiogram (ecg) were analyzed by a multi-channel physiological recorder.The myocardial enzyme activities and ion concentrations were detected by an automatic biochemical analyzer.Additionally, the levels of myocardial injury markers were established by the enzyme-linked immunosorbent assay (elisa), and those of hormones were measured by radioimmunoassay.The structure and ultrastructure of the myocardial tissue were observed using an optical microscope and transmission electron microscopy (tem).The expression of hsp72 was measured by western blot and immunofluorescence analyses.Post-exposure, the rectal temperature in the r-group increased significantly, ecg was disordered, and the concentrations of ions were decreased.Furthermore, the activities of myocardial enzymes were changed, and the contents of myocardial injury markers and hormones were increased.We observed damage to the structure and ultrastructure and significantly increased expression of hsp72.As a whole, the results indicated that s-wave microwave radiation at 30 m w/cm2 for 35 min resulted in damage to the cardiac functionality organigram, caused by a combination of the thermal and nonthermal effects.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Hsa_circRNA_0008028 Deficiency Ameliorates High Glucose-Induced Proliferation, Calcification, and Autophagy of Vascular Smooth Muscle Cells via miR-182-5p/TRIB3 Axis

It is well-known that dysfunctions of vascular smooth muscle cells (vsmcs) act an essential part in vascular complications of diabetes.Studies have shown that circular rnas (circ rnas) and micro rnas (mi rnas) play a crucial role in regulating cell functions.However, their influence on the proliferation, calcification, and autophagy of vsmcs remains to be further explored.Therefore, this study elucidates the role and mechanism of hsa_circ rna_0008028 in high glucose- (hg-, 30 m m) treated vsmcs in vitro.Methods.Quantitative real-time polymerase chain reaction (q rt-pcr) was chosen to detect the levels of hsa_circ rna_0008028, mi r-182-5p, and tribble 3 (trib3).Then, dual-luciferase reporter and rna immunoprecipitation (rip) assays were used to predict and verify the binding relationship between mi r-182-5p and hsa_circ rna_0008028 or trib3.Cell counting kit-8 assay, 5-ethynyl-2 ′ -deoxyuridine (ed u) staining, corresponding commercial kits, and western blotting were used to measure indexes reflecting cell viability, proliferation, calcification, and autophagy of vsmcs, respectively.Results.In hg-induced vsmcs, hsa_circ rna_0008028 and trib3 were highly expressed, whereas mi r-182-5p decreased.Meanwhile, cell proliferation, calcification, and autophagy could be repressed by silencing of hsa_circ rna_0008028.However, these effects can be eliminated by mi r-182-5p inhibition.Furthermore, it was demonstrated that hsa_circ rna_0008028 could promote the expression of trib3, a target of mi r-182-5p, by directly sponging mi r-182-5p.The expression of trib3 was suppressed by hsa_circ rna_0008028 knockout, which was rescued by mi r-182-5p inhibition.Conclusion.This study reveals that hsa_circ rna_0008028 can act as a sponge of mi r-182-5p and promote hg-induced proliferation, calcification, and autophagy of vsmcs partly by regulating trib3.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
HS3ST1 Promotes Non-Small-Cell Lung Cancer Progression by Targeting the SPOP/FADD/NF-κB Pathway

Heparan sulfate proteoglycan is a key component of cell microenvironment and plays an important role in cell‐cell interaction, adhesion, migration, and signal transduction.Heparan sulfate 3‐o‐sulfotransferase 1 (hs3st1) is a metabolic‐related gene of hs.The present study was aimed at exploring the role of hs3st1 in the progress of non‐small‐cell lung cancer (nsclc).Our results illustrated that hs3st1 promoted the malignant behaviors of nsclc cells both in vitro and in vivo .Hs3st1 was found to inhibit spot‐type zinc finger protein (spop) expression, which might inhibit the nf‐ κ b pathway activation through mediating the degradation of fas‐associated death domain protein (fadd).By analyzing nsclc patient samples, we also found increased hs3st1 expression and decreased spop expression in tumor tissues in contrast with those in adjoining normal tissues.In conclusion, hs3st1 promotes nsclc tumorigenesis by regulating spop/fadd/nf‐ κ b pathway.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Homeobox A1 Facilitates Immune Escape and Alleviates Oxidative Stress in Lung Adenocarcinoma

Recent studies have demonstrated that homeobox a1 (hoxa1) is upregulated in lung cancer due to rna modifications (n6-methyladenosine), but the specific function of hoxa1 in lung adenocarcinoma (luad) remains indistinct.Herein, we investigated the role of hoxa1 in luad biology.Methods.This study presented pancancer analysis of associations of hoxa1 with prognosis, tmb, and immune checkpoints.The expression of hoxa1 was detected in luad and normal tissues with immunohistochemistry and western blot.Through least absolute shrinkage and selection operator (lasso) analysis, hoxa1-derived gene model was conducted in luad.Correlations of hoxa1 with immune cell infiltrations, immune checkpoints, hlas, and chemotherapeutic sensitivity were evaluated.Colony formation, proliferation, and migration of luad cells with si-hoxa1 transfection were investigated, and the effects of hoxa1 on t cell exhaustion were assessed in vitro.Results.Hoxa1 expression was a risk factor of overall survival, disease-specific survival, and progression-free interval of luad.Hoxa1 exhibited prominent associations with immune cell infiltration, immune checkpoints, and hlas.Hoxa1-derived gene signature reliably and independently predicted luad outcomes.Also, high-risk cases presented increased sensitivity to cisplatin, paclitaxel, docetaxel, vinorelbine, and etoposide.Hoxa1 knockdown exhibited an inhibitory effect on proliferation and migration abilities of luad cells.Silencing hoxa1 weakened the expression of antioxidative stress markers nrf2/ho-1 and t cell exhaustion marker cd155 in luad cells.Moreover, luad cells with hoxa1 knockdown enhanced the cd8+ t cell response.Conclusion.Our data support the oncogenic function and prognostic significance of hoxa1 that facilitates immune escape and alleviates oxidative stress of luad.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
High Inflammatory Factor Levels Increase Cardiovascular Complications in Diabetic Patients Undergoing Coronary Artery Bypass Grafting

To investigate the association between inflammation and clinical outcomes of coronary artery bypass grafting (cabg) in diabetic patients.Methods .A total of 300 diabetic patients with coronary heart disease who underwent cabg were selected.Patients were divided into a group with cardiovascular events (32 in the macce group) and a group without cardiovascular events (268 in the non‐macce group) according to whether cardiovascular events occurred within 30 days.The differences in clinical parameters; serum levels of tnf‐ α , il‐6, il‐18, il‐1 β , and crp; factors associated with the occurrence of macce; and risk factors affecting the midterm all‐cause mortality of patients were compared between the two groups.Results .The serum levels of tnf‐ α , il‐6, il‐18, and crp in the macce group were significantly higher than those in the non‐macce group ( p &lt; 0.05).Gender, smoking, hyperlipidemia, duration of diabetes, and levels of tnf‐ α , il‐6, il‐18, and crp were closely related to the occurrence of macce.The kaplan‐meier survival analysis evaluation results showed that the levels of il‐6 and crp significantly affected the midterm all‐cause mortality rate ( p &lt; 0.05).Multivariate cox regression analysis showed that the advanced age, hypertension, hyperlipidemia, long duration of diabetes, elevated serum il‐6, and crp levels could be used as risk factors for midterm all‐cause mortality.Conclusions .Inflammation levels in diabetic patients are associated with complications and midterm all‐cause mortality in patients undergoing coronary artery bypass grafting.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Herpes Simplex Virus Infection Increases Beta-Amyloid Production and Induces the Development of Alzheimer’s Disease

Alzheimer’s disease, a neurodegenerative memory disease, primarily results from the formation of amyloid plaques (a β ) that gradually inhibit neuron communications.The entire mechanism of a β production remains unclear to date, and it is of particular interest among scientists to find out the exact mechanism that leads to amyloid precursor protein (app) cleavage through the amyloidogenic pathway so that effective treatments can be developed.Method.2 sets of experiments with the use of human h4‐n cell lines are proposed to fully investigate the validity of the hypothesis.All of the experiments would involve immunoblotting of a β using an anti‐a β antibody, and the results would be analyzed with the assistance of an image analyzer.A significant amount of a β would be expected to be present in the cytoplasm of cells with herpes simplex virus (hsv‐1) applied, as app endocytosis would be induced by hsv‐1, which leads to higher a β levels inside the cell.Results.In this paper, a new hypothesis is presented on how hsv‐1 infection initiates app endocytosis and causes an increase in app cleavage and a β production inside the cells.It is also hypothesized that increased a β peptides exit the cell via exocytosis, therefore, leading to the development of alzheimer’s disease.The findings will support the hypothesis if intracellular a β concentration is significantly higher after the introduction of d hsv‐1 and subsequently if extracellular a β concentration becomes higher without te nt exocytosis inhibition.Conclusion.The results of this study would provide valuable insights into the mechanisms underlying alzheimer’s disease and open new scopes of research for its potential treatments.Further studies on virus infection and the development of memory diseases should be conducted to investigate possible correlations.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Hematological Findings among COVID-19 Patients Attending King Khalid Hospital at Najran, Kingdom of Saudi Arabia

Covid‐19 is a global pandemic viral infection that has affected millions worldwide.Limited data is available on the effect of covid‐19 on hematological parameters in saudi arabia.This study is aimed at examining the role of hematological parameters among covid‐19 patients admitted to king khalid hospital in najran, saudi arabia.This is a retrospective, hospital‐based study of 514 cases who were recruited during august to october 2020.257 covid‐19 patients formed the study group, and a further 257 negative subjects formed the control group.Anemia was significantly elevated in positive subjects over controls (respectively, 64.2% and 35.8%), with patients 2.5 times more likely to be anemic ( p &lt; 0.01).Thrombocytopenia was higher in patients over controls (respectively, 62% and 38%), with patients ~1.7 times more likely to be thrombocytopenic ( p &lt; 0.01).Moreover, leukopenia was significantly higher in patients over controls (respectively, 71% and 29%), with positive subjects ~2.6 times more likely to be leukopenic.Our study results indicate that mild anemia associated with leukopenia may have diagnostic value for covid‐19.Careful assessment of hematological parameters, at baseline and throughout the disease path, will assist physicians in formulating personalized approaches to treatment and promptly offer intensive care to those in greater need.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Healthcare Biclustering-Based Prediction on Gene Expression Dataset

In this paper, we develop a healthcare biclustering model in the field of healthcare to reduce the inconveniences linked to the data clustering on gene expression.The present study uses two separate healthcare biclustering approaches to identify specific gene activity in certain environments and remove the duplication of broad gene information components.Moreover, because of its adequacy in the problem where populations of potential solutions allow exploration of a greater portion of the research area, machine learning or heuristic algorithm has become extensively used for healthcare biclustering in the field of healthcare.The study is evaluated in terms of average match score for nonoverlapping modules, overlapping modules through the influence of noise for constant bicluster and additive bicluster, and the run time.The results show that proposed fcm blustering method has higher average match score, and reduced run time proposed fcm than the existing pso‐sa and fuzzy logic healthcare biclustering methods.

Journal: BioMed Research International (2022)
Publication types: Research Article;
GLP-1 Analog Alleviated Cognitive Dysfunction in Aged Rats Anaesthetized with Sevoflurane

Postoperative cognitive dysfunction (pocd) in elderly patients undergoing general anesthesia is a major problem in the aging society.Sevoflurane is the most widely applied anesthetic in clinical practice.In this study, we investigated the effects of the glp‐1 analogue liraglutide on cognitive function in aged rats anesthetized by sevoflurane.Specifically, 48 sprague‐dawley rats were divided into the control (c) group, the liraglutide (l) group, the sevoflurane (s) group, and the sevoflurane+liraglutide (sl) group, each group with 12 rats.In the s group and the sl group, the rats were injected subcutaneously with normal saline and liraglutide after inhalation of a mixture of 3% sevoflurane and pure oxygen.In the c group and the l group, normal saline and liraglutide were injected subcutaneously into the rats after inhalation of pure oxygen.Morris water maze task was applied for the detection of spatial learning and memory in rats; he and tunel for staining; and western blot for quantifying bax, bcl‐2 expression, and examining caspase‐3 activity in hippocampal tissues as well as for revealing the antiapoptotic mechanism.Besides, the accumulation of inflammatory factors nf‐ κ b and il‐1 β in the hippocampal tissue was quantitatively studied to reveal the anti‐inflammatory mechanism.The protective effect of liraglutide on sevoflurane toxicity was the first to be confirmed in this study.Additionally, this study elucidated the mechanism of the above effect.The results of this study might be helpful to find an effective medical solution for the treatment of pocd caused by sevoflurane anesthesia.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Global Increase in Breast Cancer Incidence: Risk Factors and Preventive Measures

Breast cancer is a global cause for concern owing to its high incidence around the world.The alarming increase in breast cancer cases emphasizes the management of disease at multiple levels.The management should start from the beginning that includes stringent cancer screening or cancer registry to effective diagnostic and treatment strategies.Breast cancer is highly heterogeneous at morphology as well as molecular levels and needs different therapeutic regimens based on the molecular subtype.Breast cancer patients with respective subtype have different clinical outcome prognoses.Breast cancer heterogeneity emphasizes the advanced molecular testing that will help on‐time diagnosis and improved survival.Emerging fields such as liquid biopsy and artificial intelligence would help to under the complexity of breast cancer disease and decide the therapeutic regimen that helps in breast cancer management.In this review, we have discussed various risk factors and advanced technology available for breast cancer diagnosis to combat the worst breast cancer status and areas that need to be focused for the better management of breast cancer.

Journal: BioMed Research International (2022)
Publication types: Review Article;
Ginsenoside Rg3 Alleviates Antithyroid Cancer Drug Vandetanib-Induced QT Interval Prolongation

Inhibition of human ether‐a‐go‐go‐related gene (h erg) potassium channel is responsible for acquired long qt syndromes, which leads to life‐threatening cardiac arrhythmia.A multikinase inhibitor, vandetanib, prolongs the progression‐free survival time in advanced medullary thyroid cancer.However, vandetanib has been reported to induce significant qt interval prolongation, which limits its clinical application.Some studies have showed that ginsenoside rg3 decelerated h erg k(+) channel tail current deactivation.Therefore, in this study, we aim to confirm whether ginsenoside rg3 targeting h erg k(+) channel could be used to reverse the vandetanib‐induced qt interval prolongation.Electrocardiogram (ecg) and monophasic action potential (map) were recorded using electrophysiology signal sampling and analysis system in langendorff‐perfused rabbit hearts.The current clamp mode of the patch‐clamp technique was used to record transmembrane action potential.The whole‐cell patch‐clamp technique was used to record the h erg k + current.In langendorff‐perfused hearts, vandetanib prolonged the qt interval in a concentration‐dependent manner with an ic 50 of 1.96 μ mol/l.In human‐induced pluripotent stem cell‐derived cardiomyocytes (hi psc‐cms), vandetanib significantly prolonged the action potential duration at 50%, 70%, and 90% repolarization (apd 50 , apd 70 , and apd 90 ).In stable transfected human h erg gene hek293 cells, vandetanib caused concentrate‐dependent inhibition in the step and tail currents of h erg.As expected, ginsenoside rg3 relieved vandetanib‐induced qt interval prolongation in langendorff‐perfused heart and reversed vandetanib‐induced apd prolongation in hi psc‐cms.Furthermore, ginsenoside rg3 alleviated vandetanib‐induced h erg current inhibition and accelerated the process of the channel activation.Ginsenoside rg3 may be a promising cardioprotective agent against vandetanib‐induced qt interval prolongation through targeting h erg channel.These novel findings highlight the therapeutic potential of ginsenoside to prevent vandetanib‐induced cardiac arrhythmia.

Journal: Oxidative Medicine and Cellular Longevity (2021)
Publication types: Research Article;
Gauging the Impact of Artificial Intelligence and Mathematical Modeling in Response to the COVID-19 Pandemic: A Systematic Review

While the world continues to grapple with the devastating effects of the sars‐n co v‐2 virus, different scientific groups, including researchers from different parts of the world, are trying to collaborate to discover solutions to prevent the spread of the covid‐19 virus permanently.Henceforth, the current study envisions the analysis of predictive models that employ machine learning techniques and mathematical modeling to mitigate the spread of covid‐19.A systematic literature review (slr) has been conducted, wherein a search into different databases, viz., pub med and ieee explore, fetched 1178 records initially.From an initial of 1178 records, only 50 articles were analyzed completely.Around (64%) of the studies employed data‐driven mathematical models, whereas only (26%) used machine learning models.Hybrid and arima models constituted about (5%) and (3%) of the selected articles.Various quality evaluation metrics (qem), including accuracy, precision, specificity, sensitivity, brier‐score, f1‐score, rmse, auc, and prediction and validation cohort, were used to gauge the effectiveness of the studied models.The study also considered the impact of pfizer‐bio ntech (bnt162b2), astra zeneca (ch ad0x1), and moderna (m rna‐1273) on beta (b.1.1.7) and delta (b.1.617.2) viral variants and the impact of administering booster doses given the evolution of viral variants of the virus.

Journal: BioMed Research International (2022)
Publication types: Review Article;
Foreign Muslim Workers’ Perspectives of the Basic Needs of Muslim-Friendly Tourist Services: An Empirical Analysis of a Non-Muslim Destination

The rapid growth of halal travel offers a tourism opportunity for countries, but non-muslim countries often find it difficult to meet the multiple religious needs of muslim tourists.This study verifies muslim tourists’ requirements for basic hotel and food services and identifies the categories in which to place these attributes and which ones need resources to increase muslim tourists’ satisfaction.Applying taiwan as a non-muslim destination, we surveyed 216 indonesian muslim foreign workers and used the recently developed gradient boosting decision trees, instead of a regression method, to overcome the nonlinear and multicollinearity issues of penalty-reward-contrast analysis.Impact-range performance analysis and asymmetric impact-performance analysis were then applied to examine the basic attributes of muslim foreign workers’ tourist experiences.We learned that using gradient boosting decision trees provided advantages over the regression method.Empirical results indicated that hotel-related factors are prioritized over food services when muslim tourists visit a non-muslim country.In addition, religious observances play an essential part in muslim tourists’ hotel choices, and conveniently accessible halal food is a desirable travel requisite for this group.

Journal: Security and Communication Networks (2022)
Publication types: Research Article;