syedzayyan/paper_mill_benchmark huggingface dataset:
The risk of transmission of the novel coronavirus (SARS-CoV-2) with human heart valve transplantation: evaluation of cardio-vascular tissues from two consecutive heart donors with asymptomatic COVID-19.

We report on two living donors of explanted hearts while receiving heart transplantation that tested positive for sars-co v-2 on the day of donation, although clinically asymptomatic.They underwent heart transplantation for ischaemic and hypertrophic obstructive cardiomyopathy, respectively.After evaluation of donor hearts, we cryopreserved and stored two pulmonary valves for clinical application and one aortic valve for research.Light microscopy of myocardium, mitral valve and aortic and pulmonary arterial wall and rt-pcr sars-co v-2 test of myocardium, mitral and tricuspid valve and aortic wall for detection of sars-co v-2 were performed.Presence of ace2 in tissues was assessed with immunostaining.Light microscopy revealed a mild eosinophilic myocarditis in the ischemic cardiomyopathy heart, whereas enlarged cardiomyocytes with irregular nucleus and some with cytoplasmic vacuoles in the hypertrophic obstructive cardiomyopathy heart.Aortic and pulmonary wall were histologically normal.Immunostaining revealed diffuse presence of ace2 in the myocardium of the heart with eosinophilic myocarditis, but only discrete presence in the hypertrophic cardiomyopathy heart.The rt-pcr sars-co v-2 test showed no presence of the virus in tested tissues.Despite eosinophilic myocarditis in the ischemic cardiomyopathy heart, no viral traces were found in the myocardium and valve tissues.However, ace2 was present diffusely in the ischemic cardiomyopathy heart.Sars-co v-2 could not be detected in the cardiac tissues of these covid-19 asymptomatic heart donors.In our opinion, clinical application of the valves from these donors presents negligible risk for coronavirus transmission.Nonetheless, considering the uncertainty regarding the risk of virus transmission with the human tissue transplantation, we would not release in any case the pulmonary valve recovered from the eosinophilic myocarditis heart.In contrast, we may consider the release of the pulmonary valve from the dilated cardiomyopathy heart only for a life-threatening situation when no other similar allograft were available.

Journal: Cell and tissue banking (2021)
Publication types: Case Reports;Journal Article
The polybasic insert, the RBD of the SARS-CoV-2 spike protein, and the feline coronavirus - evolved or yet to evolve.

Recent research on the sars-co v-2 pandemic has exploded around the furin-cleavable polybasic insert prrar↓s, found within the spike protein.The insert and the receptor-binding domain, (rbd), are vital clues in the sherlock holmes-like investigation into the origin of the virus and in its zoonotic crossover.Based on comparative analysis of the whole genome and the sequence features of the insert and the rbd domain, the bat and the pangolin have been proposed as very likely intermediary hosts.In this study, using the various databases, in-house developed tools, sequence comparisons, structure-guided docking, and molecular dynamics simulations, we cautiously present a fresh, theoretical perspective on the sars-co v-2 virus activation and its intermediary host.They are a) the sars-co v-2 has not yet acquired a fully optimal furin binding site or this seemingly less optimal sequence, prrars, has been selected for survival; b) in structural models of furin complexed with peptides, prrar↓s binds less well and with distinct differences as compared to the all basic rrkrr↓s; c) these differences may be exploited for the design of virus-specific inhibitors; d) the novel polybasic insert of sars-co v-2 may be promiscuous enough to be cleaved by multiple enzymes of the human airway epithelium and tissues which may explain its unexpected broad tropism; e) the rbd domain of the feline coronavirus spike protein carries residues that are responsible for high-affinity binding of the sars-co v-2 to the ace 2 receptor; f)en routezoonotic transfer, the virus may have passed through the domestic cat whose very human-like ace2 receptor and furin may have played some role in optimizing the traits required for zoonotic transfer.

Journal: Biochemistry and biophysics reports (2021)
Publication types: Journal Article
Theory-Driven Design of a Cationic Accelerator for High-Performance Electrolytic MnO2-Zn Batteries.

Aqueous electrolytic mn o2-zn batteries are considered as one of the most promising energy-storage devices for their cost effectiveness, high output voltage, and safety, but their electrochemical performance is limited by the sluggish kinetics of cathodic mn o2/mn2+and anodic zn/zn2+reactions.To overcome this critical challenge, herein, a cationic accelerator (ca) strategy is proposed based on the prediction of first-principles calculations.Poly(vinylpyrrolidone) is utilized as a model to testify the rational design of the ca strategy.It manifests that the ca effectively facilitates rapid cations migration in electrolyte and adequate charge transfer at electrode-electrolyte interface, benefiting the deposition/dissolution processes of both mn2+and zn2+cations to simultaneously improve kinetics of cathodic mn o2/mn2+and anodic zn/zn2+reactions.The resulting mn o2-zn battery regulated by ca exhibits large reversible capacities of 455 m ah g-1and 3.64 m ah cm-2at 20 c, as well as a long lifespan of 2000 cycles with energy density retention of 90%, achieving one of the best overall performances in the electrolytic mn o2-zn batteries.This comprehensive work integrating theoretical prediction with experimental studies provides opportunities to the development of high-performance energy-storage devices.

Journal: Advanced materials (Deerfield Beach, Fla.) (2022)
Publication types: Journal Article
Children against antibiotics misuse and antimicrobial resistance: assessing effectiveness of storytelling and picture drawing as public engagement approaches.

Interventions delivered in schools have been found to be effective in improving knowledge of antibiotics and antimicrobial resistance (amr) among school-aged children, particularly those in high-income countries, but the evidence is largely lacking in low- and middle-income countries.This study aimed to design, implement and assess storytelling and picture drawing as engagement approaches for improving knowledge, attitudes and beliefs about antibiotics and amr among schoolchildren in ghana.methods: two schools with a total population of 375 schoolchildren ages 11-15 years in tema, a city in ghana, participated in public engagement interventions involving storytelling in one school and picture drawing in another school.The interventions included eight weeks of engagement led by science teachers and a competition held in each school.For quantitative outcome-based evaluation, some schoolchildren were randomly sampled in each school.Purposive sampling was also used to select some schoolchildren in each school for qualitative outcome-based evaluation.Respondents completed identical knowledge, attitudes and beliefs questionnaires and were interviewed at two time points (before and at most a week) after key interventions to assess changes in antibiotics and amr knowledge, attitudes and beliefs.results:picture drawing had more significant effects (both positive and negative) on schoolchildren's amr knowledge, attitudes and beliefs, whereas storytelling had a negative effect on children's amr knowledge and no significant impact on beliefs and attitudes.conclusions:our project's findings suggest that public engagement interventions that use picture drawing and storytelling may influence the knowledge, attitudes and beliefs of schoolchildren regarding antibiotic misuse and amr.However, modifications are required to make them more effective.These include making the storytelling effective by turning it into drama or plays.

Journal: Wellcome open research (2021)
Publication types: Journal Article
Prognostic value of post-percutaneous coronary intervention diastolic pressure ratio.

To evaluate the distribution of a generic diastolic pressure ratio (d pr) after angiographically successful percutaneous coronary intervention (pci) and to assess its association with the 2‐year incidence of target vessel failure (tvf), defined as a composite of cardiac mortality, target vessel revascularisation, target vessel myocardial infarction and stent thrombosis.Methods the d pr search study is a post hoc analysis of the prospective single-centre ffr-search registry, in which physiological assessment was performed after angiographically successful pci in a total of 1000 patients, using a dedicated microcatheter.D pr was calculated offline with recently validated software in a subset of 735 patients.Results mean post-pci d pr was 0.95 ± 0.06.Post-pci d pr was ≤ 0.89 in 15.2% of the patients.The cumulative incidence of tvf at 2‐year follow-up was 9.4% in patients with a final post-pci d pr ≤ 0.89 as compared to 6.1% in patients with a post-pci d pr > 0.89 (adjusted hazard ratio [hr] for d pr ≤ 0.89: 1.53; 95% ci 0.74-3.13; p = 0.249).D pr ≤ 0.89 was associated with significantly higher cardiac mortality at 2 years; adjusted hr 2.40; 95% ci 1.01-5.68; p = 0.047.Conclusions in a real-world setting, despite optimal angiographic pci results, 15.2% of the patients had a final post-pci d pr of ≤ 0.89, which was associated with a higher incidence of tvf and a significantly higher cardiac mortality rate.

Journal: Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation (2022)
Publication types: Journal Article
Oxidative stress in leukemia and antioxidant treatment.

Oxidative stress is caused by the imbalance between the generation of free radicals/reactive oxygen species (ros) and the antioxidant defense systems, which can activate various transcription factors and affect their transcriptional pathways.Oxidative stress plays an important role in the occurrence and development of leukemia and is closely related to the treatment and prognosis of leukemia.The standard chemotherapy strategies for the pre-treatment of leukemia have many drawbacks.Hence, the usage of antioxidants and oxidants in the treatment of leukemia is being explored and has been preliminarily applied.This article reviews the research progress of oxidative stress and leukemia.In addition, the application of antioxidants treatment in leukemia has been summarized.

Journal: Chinese medical journal (2021)
Publication types: Journal Article;Review
An exceptional survival in an unoperated tetralogy of Fallot in a 66-year-old man: A case report.

Tetralogy of fallot is the most common cyanotic congenital heart disease in children which comprises an overriding aorta, right ventricular outflow obstruction, ventricular septal defect, and right ventricular hypertrophy.It has an elevated early mortality rate without surgical correction, with most patients dying in childhood.Only 2% of patients survive past the age of 40 years without surgical intervention.Very few cases of survival to middle age have been reported, particularly after the fourth decade.In this article, we present a case of a 66-year-old male with an unoperated tetralogy of fallot, which is one of the longest time periods of diagnosis.Despite tetralogy and having right ventricular dysfunction, this patient presented with fatigue, exertional dyspnea, cyanosis, and systemic hypertension.Considering the patient's comorbid conditions and the risk associated with the surgery, the patient was managed conservatively.To our knowledge, this is the oldest unoperated tetralogy of fallot case reported in nepal.

Journal: Radiology case reports (2022)
Publication types: Case Reports;Journal Article
Interventional Procedures for Left Ventricular Assist Device-Associated Complications.

As patients on long-term left ventricular assist device (lvad) face a substantial risk for open cardiac reoperation, interventional treatment approaches are becoming increasingly important in this population.We evaluated data of 871 patients who were on lvad support between january 1, 2016 and december 1, 2020.Interventional treatments for lvad-associated complications were performed in 76 patients.Seventeen patients underwent transcatheter aortic valve replacements (tavr) and 61 patients underwent outflow graft interventions (ogi).Tavr improved symptoms in patients with severe symptomatic aortic regurgitation.Postinterventional complications included aggravation of preexisting right heart failure (rhf), third-degree atrioventricular block, and intrapump thrombosis (in 3 [16.7%], 2 [11.1%], and 1 [5.6%] patients, respectively).In outflow graft obstructions, ogi led to recovery of lvad flow ( p < 0.001), unloading of the left ventricle ( p = 0.004), decrease of aortic valve opening time ( p = 0.010), and improvement of right heart function ( p < 0.001).Complications included bleeding, rhf, and others (in 9 [10.8%], 5 [6.0%], and 5 [6.0%] patients, respectively).Eight (9.6%) patients died within the hospital stay after ogi, including mortality secondary to prolonged cardiogenic shock.In conclusion, interventional procedures are a feasible and safe treatment modality for lvad-associated complications.

Journal: ASAIO journal (American Society for Artificial Internal Organs : 1992) (2022)
Publication types: Journal Article
A dietary pattern high in full-fat dairy and sweetened beverages is associated with glycated hemoglobin and weight in Mexican patients with type-2 diabetes.

The aim of this study was to identify dietary patterns in a sample of patients with type-2 diabetes, and to evaluate their association with markers of metabolic control.Methods: a cross-sectional study in 395 patients with type-2 diabetes in primary care was conducted.Fasting blood levels of glycated hemoglobin (a1c), glucose, total cholesterol, low- (ldl-c) and high-density lipoprotein cholesterol (hdl-c), and triglycerides were measured.Waist circumference, body mass index (bmi), and blood pressure were evaluated.Dietary intake was assessed by a food frequency questionnaire, and dietary patterns were derived by cluster analysis.Three dietary patterns were identified: 'fruits and vegetables', 'dairy and sweetened beverages', and 'diverse with alcohol'.Results: an association between the 'dairy and sweetened beverages' dietary pattern and a1c levels was identified (ß = 0.61; 95 % ci: 0.09, 1.12, p = 0.021), considering the 'fruits and vegetables' dietary pattern as the reference group.We also observed a trend towards an adjusted increased risk of a1c ≥ 7 % (odds ratio [or]: 1.56; 95 % ci: 0.92, 2.64; p = 0.099) and an increased risk of bmi ≥ 25 kg/m2 (or: 2.62, 95 % ci: 1.20, 5.71, p = 0.015) among patients in the 'dairy and sweetened beverages' dietary pattern as compared to the reference group.Conclusions: a dietary pattern characterized by a high intake of full-fat dairy and sweetened beverages was associated with higher a1c levels and increased risk of high glucose and bmi when compared to a dietary pattern with a higher consumption of fruits and vegetables.

Journal: Nutricion hospitalaria (2022)
Publication types: Journal Article
Modular hyperthermostable bacterial endo-β-1,4-mannanase: molecular shape, flexibility and temperature-dependent conformational changes.

Endo-β-1,4-mannanase from thermotoga petrophila (tp man) is a hyperthermostable enzyme that catalyzes the hydrolysis of β-1,4-mannoside linkages in various mannan-containing polysaccharides.A recent study reported that tp man is composed of a gh5 catalytic domain joined by a linker to a carbohydrate-binding domain.However, at this moment, there is no three-dimensional structure determined for tp man.Little is known about the conformation of the tp man as well as the role of the length and flexibility of the linker on the spatial arrangement of the constitutive domains.In this study, we report the first structural characterization of the entire tp man by small-angle x-ray scattering combined with the three-dimensional structures of the individual domains in order to shed light on the low-resolution model, overall dimensions, and flexibility of this modular enzyme at different temperatures.The results are consistent with a linker with a compact structure and that occupies a small volume with respect to its large number of amino acids.Furthermore, at 20°c the results are consistent with a model where tp man is a molecule composed of three distinct domains and that presents some level of molecular flexibility in solution.Even though the full enzyme has some degree of molecular flexibility, there might be a preferable conformation, which could be described by the rigid-body modeling procedure.Finally, the results indicate that tp man undergoes a temperature-driven transition between conformational states without a significant disruption of its secondary structure.Our results suggest that the linker can optimize the geometry between the other two domains with respect to the substrate at high temperatures.These studies should provide a useful basis for future biophysical studies of entire tp man.

Journal: PloS one (2014)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Nanotechnology advances for sustainable agriculture: current knowledge and prospects in plant growth modulation and nutrition.

Advances in nanotechnology make it an important tool for improving agricultural production.Strong evidence supports the role of nanomaterials as nutrients or nanocarriers for the controlled release of fertilizers to improve plant growth.Scientific research shows that nanotechnology applied in plant sciences is smart technology.Excessive application of mineral fertilizers has produced a harmful impact on the ecosystem.Furthermore, the projected increase in the human population by 2050 has led to the search for alternatives to ensure food security.Nanotechnology is a promising strategy to enhance crop productivity while minimizing fertilizer inputs.Nanofertilizers can contribute to the slow and sustainable release of nutrients to improve the efficiency of nutrient use in plants.Nanomaterial properties (i.e., size, morphology and charge) and plant physiology are crucial factors that influence the impact on plant growth.An important body of scientific research highlights the role of carbon nanomaterials, metal nanoparticles and metal oxide nanoparticles to improve plant development through the modulation of physiological and metabolic processes.Modulating nutrient concentrations, photosynthesis processes and antioxidant enzyme activities have led to increases in shoot length, root development, photosynthetic pigments and fruit yield.In parallel, nanocarriers (nanoclays, nanoparticles of hydroxyapatite, mesoporous silica and chitosan) have been shown to be an important tool for the controlled and sustainable release of conventional fertilizers to improve plant nutrition; however, the technical advances in nanofertilizers need to be accompanied by modernization of the regulations and legal frameworks to allow wider commercialization of these elements.Nanofertilizers are a promising strategy to improve plant development and nutrition, but their application in sustainable agriculture remains a great challenge.The present review summarizes the current advance of research into nanofertilizers, and their future prospects.

Journal: Planta (2021)
Publication types: Journal Article;Review
Anti-Colorectal Cancer Effects ofInonotus hispidus(Bull.: Fr.) P. Karst. Spore Powder through Regulation of Gut Microbiota-Mediated JAK/STAT Signaling.

Inonotus hispidus(bull.: fr.) p.Karst.Spore powder (ihs) contains polyphenols and triterpenoids with pharmacological effects.Here, we analyzed its composition, and we investigated the effects of ihs on colorectal cancer (crc) in b6/jgpt-apcem1cin(min)/gpt (apc min/+) mice and its potential mechanisms by analyzing gut microbiota and serum metabolomics.The enzyme-linked immunosorbent assays and western blotting were used to confirm the changes in the cytokine and protein levels associated with ihs administration.The ihs affected the abundance of gut microbiota and the level of l-arginine (l-arg).Furthermore, the ihs influenced t cells in apc min/+mice by increasing the interleukin (il)-2 and decreasing the il-5, -6, and -10 levels, thus suppressing tumor development.Overall, ihs showed anti-crc properties in apc min/+mice by affecting the gut microbiota and serum metabolites, which in turn affected the janus kinase (jak)/signal transducer and activator of transcription (stat) signaling, and regulated the abundance of cd8+t cells.These results provide experimental support for the potential future treatment of crc with ihs.

Journal: Nutrients (2022)
Publication types: Journal Article
Soil Mite (Acari: Mesostigmata) Communities and Their Relationships with Some Environmental Variables in Experimental Grasslands from Bucegi Mountains in Romania.

The main objective of the study was to analyse, for the first time in romania, the relationships between five experimental grasslands and mesostigmata fauna, considering: (1) the structural differences between mite communities; (2) the variations in some important abiotic factors (vegetation cover, soil temperature, soil moisture content, p h, soil resistance at penetration); and (3) the influence of these abiotic factors on the structures of mesostigmata mite communities and the dispersal rates of these communities between the investigated plots.In total, 250 soil samples were analysed in july 2017, revealing the presence of 30 species, with 1163 individuals.Using the multivariate analysis, we observed that each experimental plot was defined by characteristic environmental conditions, i.e., vegetation cover, soil moisture content, and soil temperature differed significantly between the experimental grasslands.Each experimental plot was characterised by a specific indicator species and population parameters (numerical abundance and species richness).The effects of these soil variables were even demonstrated at species level:veigaia planicola,geolaelaps nolli,and gamasellodes insigniswere influenced by vegetation cover,lysigamasus conusand dendrolaelapsfoveolatusby soil temperature.The dispersal rates of mite communities from one plot to another were higher in the grasslands, where there were euconstant-constant species.

Journal: Insects (2022)
Publication types: Journal Article
Photocatalyst and additive-free visible light induced trifluoromethylation-arylation ofN-arylacrylamides with Umemoto's reagent.

A visible light induced highly convenient and practical method for the trifluoromethyl-arylation of n-arylmethacrylamides has been developed using umemoto's reagent as the trifluoromethyl source.This user-friendly approach can proceed under visible light irradiation without any transition metal, photocatalyst and additive at room temperature.The strategy presented here provides access to trifluoromethylated oxindoles in good to excellent yields with a broad functional group tolerance.Preliminary mechanistic experiments indicated that the reaction process involves a homolytic cleavage of umemoto's reagent irradiated by visible light.

Journal: Chemical communications (Cambridge, England) (2021)
Publication types: Journal Article