syedzayyan/paper_mill_benchmark huggingface dataset:
Decorin promotes decidual M1-like macrophage polarization via mitochondrial dysfunction resulting in recurrent pregnancy loss.

Rationale:recurrent pregnancy loss (rpl) is a distressing disorder that seriously affects the physical and psychological health of women.Rpl is also a sentinel risk marker for future obstetric complications and warrants in-depth investigation.Abnormal polarization and functions of decidual macrophages are associated with rpl; however, the underlying mechanisms remain poorly understood.methods:decorin expression, localization, and content in the decidua of women with normal pregnancy (np) and those with rpl were measured using reverse transcription-quantitative polymerase chain reaction (rt-q pcr), western blotting, immunofluorescence, and enzyme-linked immunosorbent assay.The profiles of decidual macrophage subsets were determined using flow cytometry and immunofluorescence in both groups.The correlation between decorin content and the proportion of decidual macrophage subsets in the decidua of early np women was determined using pearson analysis.The effects of decorin on the polarization and functions of macrophages were assessed in anin-vitromodel of raw264.7 cells via flow cytometry, western blotting, and rt-q pcr.Moreover, the mitochondrial metabolism in raw264.7 cells under decorin administration was measured via flow cytometry, western blotting, and immunofluorescence.Thirty-three pregnant mice were included in thein vivomodel and underwent different treatments.The embryo abortion rate, macrophage phenotype in the spleen and uteri, and placental development were evaluated using flow cytometry and hematoxylin-eosin staining.results:decorin, derived from decidual stromal cells, was highly expressed in the decidua of women with rpl.A positive correlation between decorin content and the proportion of m1-like macrophages was also observed in the decidua of early np women.in vitrostudies showed that decorin treatment inhibited macrophage polarization to m2-like subsets and boosted the inflammatory response, which was related to enhanced anaerobic glycolysis, increased mitochondrial membrane potential and intracellular reactive oxygen species levels, reduced mitochondrial mass, and activation of the myeloid differentiation primary response 88-nuclear factor-κb signaling pathway.Adoptive transfer of decorin-treated bone marrow-derived macrophages in pregnant c57bl/6 mice increased the embryo absorption, accompanied by impaired fetal vascularization.conclusions:decidual stromal cell-derived decorin can polarize decidual macrophages toward the m1 phenotype by regulating mitochondrial metabolism, resulting in the occurrence of rpl.

Journal: Theranostics (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Sonothermogenetics for noninvasive and cell-type specific deep brain neuromodulation.

Critical advances in the investigation of brain functions and treatment of brain disorders are hindered by our inability to selectively target neurons in a noninvasive manner in the deep brain.Objective this study aimed to develop sonothermogenetics for noninvasive, deep-penetrating, and cell-type-specific neuromodulation by combining a thermosensitive ion channel trpv1 with focused ultrasound (fus)-induced brief, non-noxious thermal effect.Methods the sensitivity of trpv1 to fus sonication was evaluated in vitro.It was followed by in vivo assessment of sonothermogenetics in the activation of genetically defined neurons in the mouse brain by two-photon calcium imaging.Behavioral response evoked by sonothermogenetic stimulation at a deep brain target was recorded in freely moving mice.Immunohistochemistry staining of ex vivo brain slices was performed to evaluate the safety of fus sonication.Results trpv1 was found to be an ultrasound-sensitive ion channel.Fus sonication at the mouse brain in vivo selectively activated neurons that were genetically modified to express trpv1.Temporally precise activation of trpv1-expressing neurons was achieved with its success rate linearly correlated with the peak temperature within the fus-targeted brain region as measured by in vivo magnetic resonance thermometry.Fus stimulation of trpv1-expressing neurons at the striatum repeatedly evoked locomotor behavior in freely moving mice.Fus sonication was confirmed to be safe based on inspection of neuronal integrity, inflammation, and apoptosis markers.Conclusions this noninvasive and cell-type-specific neuromodulation approach with the capability to stimulate deep brain has the promise to advance the study of the intact nervous system and uncover new ways to treat neurological disorders.

Journal: Brain stimulation (2021)
Publication types: Journal Article;Research Support, N.I.H., Extramural;Research Support, Non-U.S. Gov't
A new pancreatic adenocarcinoma-derived organoid model of acquired chemoresistance to FOLFIRINOX: First insight of the underlying mechanisms.

Information although improvements have been made in the management of pancreatic adenocarcinoma (pdac) during the past 20 years, the prognosis of this deadly disease remains poor with an overall 5-year survival under 10%.Treatment with folfirinox, a combined regimen of 5-fluorouracil, irinotecan (sn-38) and oxaliplatin, is nonetheless associated with an excellent initial tumour response and its use has allowed numerous patients to go through surgery while their tumour was initially considered unresectable.These discrepancies between initial tumour response and very low long-term survival are the consequences of rapidly acquired chemoresistance and represent a major therapeutic frontier.To our knowledge, a model of resistance to the combined three drugs has never been described due to the difficulty of modelling the folfirinox protocol both in vitro and in vivo.Patient-derived tumour organoids (pdo) are the missing link that has long been lacking in the wide range of epithelial cancer models between 2d adherent cultures and in vivo xenografts.In this work we sought to set up a model of pdo with resistance to folfirinox regimen that we could compare to the paired naive pdo.Results we first extrapolated physiological concentrations of the three drugs using previous pharmacodynamics studies and bi-compartmental elimination models of oxaliplatin and sn-38.We then treated pa ta-1818x naive pdac organoids with six cycles of 72 h-folfirinox treatment followed by 96 h interruption.Thereafter, we systematically compared treated organoids to pa ta-1818x naive organoids in terms of growth, proliferation, viability and expression of genes involved in cancer stemness and aggressiveness.Conclusions we reproductively obtained resistant organoids fox r that significantly showed less sensitivity to folforinox treatment than the pa ta-1818x naive organoids from which they were derived.Our resistant model is representative of the sequential steps of chemoresistance observed in patients in terms of growth arrest (proliferation blockade), residual disease (cell quiescence/dormancy) and relapse.Significance to our knowledge, this is the first genuine in vitro model of resistance to the three drugs in combined therapy.This new pdo model will be a great asset for the discovery of acquired chemoresistance mechanisms, knowledge that is mandatory before offering new therapeutic strategies for pancreatic cancer.

Journal: Biology of the cell (2022)
Publication types: Journal Article
Detection of Oculomotor Dysmetria From Mobile Phone Video of the Horizontal Saccades Task Using Signal Processing and Machine Learning Approaches.

Eye movement assessments have the potential to help in diagnosis and tracking of neurological disorders.Cerebellar ataxias cause profound and characteristic abnormalities in smooth pursuit, saccades, and fixation.Oculomotor dysmetria (i.e., hypermetric and hypometric saccades) is a common finding in individuals with cerebellar ataxia.In this study, we evaluated a scalable approach for detecting and quantifying oculomotor dysmetria.Eye movement data were extracted from i phone video recordings of the horizontal saccade task (a standard clinical task in ataxia) and combined with signal processing and machine learning approaches to quantify saccade abnormalities.Entropy-based measures of eye movements during saccades were significantly different in 72 individuals with ataxia with dysmetria compared with 80 ataxia and parkinson's participants without dysmetria.A template matching-based analysis demonstrated that saccadic eye movements in patients without dysmetria were more similar to the ideal template of saccades.A support vector machine was then used to train and test the ability of multiple signal processing features in combination to distinguish individuals with and without oculomotor dysmetria.The model achieved 78% accuracy (sensitivity= 80% and specificity= 76%).These results show that the combination of signal processing and machine learning approaches applied to i phone video of saccades, allow for extraction of information pertaining to oculomotor dysmetria in ataxia.Overall, this inexpensive and scalable approach for capturing important oculomotor information may be a useful component of a screening tool for ataxia and could allow frequent at-home assessments of oculomotor function in natural history studies and clinical trials.

Journal: IEEE access : practical innovations, open solutions (2022)
Publication types: Journal Article
New On-Water Test for the Assessment of Blood Lactate Response to Exercise in Elite Kayakers.

Lactate thresholds are physiological parameters used to train athletes and monitor performance or training.Currently, the assessment of lactate thresholds in kayakers is performed in a laboratory setting utilizing specific ergometers; however, laboratory tests differ from on-water evaluation for several reasons.The aim of this study was to assess reliability and validity of a new on-water incremental test for the assessment of blood lactate response to exercise in flat-water kayakers.Maximal lactate steady state test (mlss) was used as criterion measurement.Methods eleven junior (16.5 ± 1.9 yr) élite flat-water kayakers performed: i) an incremental cardiopulmonary test up to voluntary exhaustion on a stationary kayak ergometer to determine peak oxygen uptake; ii) an on-water 1000-m distance trial (t1000) to record best performance time and average speed (s1000); iii) two repetitions of on-water incremental kayaking test (wik test); iv) several repetitions of on-water constant speed tests to determine mlss.Speed, hr, and blood lactate concentrations were determined during on-water tests.Results the best performance time in t1000 was 262 ± 13 s, corresponding to an s1000 of 3.82 ± 0.19 m·s.Lactate threshold determined by modified dmax method (ltdmod) during wik test was 2.78 ± 1.02 mmol·l and the corresponding speed (slt) was 3.34 ± 0.16 m·s.Test-retest reliability, calculated on slt, was strong (icc = 0.95 and r = 0.93).Mlss test corresponded to 3.06 ± 0.68 mmol·l and was reached at a speed (smlss) of 3.36 ± 0.14 m·s.Correlation coefficient between slt and smlss was 0.90 (p = 0.0001).Interestingly, a significant correlation (r = 0.96, p < 0.0001) was observed between slt and s1000.Conclusions the wik test showed good reliability and validity for the assessment of speed corresponding to ltdmod in flat-water kayakers and it could be a useful tool to monitor athletic performance.The speed value at ltdmod nicely predicted performance on 1000 m.

Journal: Medicine and science in sports and exercise (2019)
Publication types: Journal Article
A structured mentorship program for medical college faculty on health research.

A paradigm shift is required in faculty development programs on research skills, from theory-driven to hands-on practical approach.The objective of this study was to develop and implement a structured mentorship model for training of medical faculties in research skills.Methods an interventional study using functional mentorship and experiential learning based on a research project was conducted over a period of one year through two prevalidated modules: protocol and manuscript writing.We included early and mid-career medical faculty as mentees (mentor:mentee ratio-1:2).Module 1 consisted of eight days of active learning and 25 days of refinement period-the end point being submission of research proposal to the ethics committee.Module 2 consisted of six days of active learning and 15 days of refinement period with the end point being manuscript submission to a peer-reviewed journal.Context, input, process and product model of evaluation was used for this program.Results all eight faculty who participated as mentees in this program completed the first module, developed protocols under this program and processed them through the ethics committee.Six of the eight participants of this original cohort attended the second module and five could submit their manuscript to a peer-reviewed journal within the stipulated date.Participants expressed improvement in their self-rating of research skills, satisfaction with the program and an overall favourable change in attitude towards research.Conclusion structured mentorship program with the help of local mentors could enhance research skills of medical faculty.

Journal: Medical journal, Armed Forces India (2021)
Publication types: Journal Article
Development of activated carbon for removal of pesticides from water: case study.

The work primarily concerns development of activated carbon dedicated for adsorption of pesticides from water prior directing it to the distribution system.We provide an information on research on important practical aspects related to research carried out to develop and to manufacture activated carbons.The paper concerns preliminary works on selection raw materials, a binder used for producing granulated adsorbent, activating gases, conditions of the production process, and others.The key attention in this research was paid to its target, i.e., industrial process to produce activated carbon revealing fulfilling required properties including satisfying adsorption of selected pesticides and meeting the requirements of companies dealing with a large-scale production of drinking water.Therefore, among others, the work includes considerations concerning such aspects like pore structure and specific surface area of the activated carbon, formation of granules that are the most demanded and thus preferred in an industrial practice form of activated carbons, and other aspects important from practical point of view.Using the results of our preliminary work, a batch of granular activated carbon was produced in industrial conditions.The obtained material was tested in terms of removing several pesticides at a water treatment plant operating on an industrial scale.During tests the concentration of acetochlor esa was decreased from ca.0.4 μg/l in raw water to below 0.1 μg/l.During 11 months of ac use specific surface area of adsorbent lowered significantly by 164 m2/g, and total pore volume declined from initial 0.56 cm3/g to 0.455 cm3/g.We discuss both a performance of the obtained activated carbon in a long-term removal of acetochlor and its derivatives from water and an effect of exploitation time on the removal efficiency.The explanations for the reduction in pesticide removal efficiency are also proposed and discussed.

Journal: Scientific reports (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Open wound and cutaneous fistulization after microwave ablation of hepatocarcinoma.

A 64-year-old male with a history of hbv child a meld 9 cirrhosis on treatment with oral entecavir 0.5 mg/day.Diagnosed with 2 cm hepatocarcinoma in segment 6 treated by radiofrequency in april 2016 with complete response until june 2021, when an increase in the size of the solid component of the treated lesion (28 x 20 mm in diameter) was detected with early enhancement after the administration of paramagnetic contrast, suggestive of local tumor recurrence.Microwave ablation is a percutaneous thermal treatment that creates an electromagnetic field around a monopolar electrode, inducing homogeneous heating and coagulative tissue necrosis.It allows treating several lesions simultaneously and in less time than radiofrequency ablation with low morbidity and mortality.The incidence of adverse events ranges between 2.6% and 7.5%.The most frequent complications are bleeding and hematoma.Ablation tract fistulization is an infrequent complication, with a higher risk of appearing in subcapsular or peripheral hepatic lesions, as was the case in our patient.

Journal: Revista espanola de enfermedades digestivas (2022)
Publication types: Case Reports;Letter
Brazilian smokers are ready for the ban on flavour additives in tobacco to be implemented.

Brazil became the first country to approve a national policy to ban all flavour additives in all tobacco leaf products in 2012.However, as of february 2022, the policy remained to be implemented.Cross-sectional data come from the international tobacco control (itc) brazil wave 3 survey among adult smokers (n = 1216) in 2016-2017.The majority of smokers supported a ban on menthol (56.0%; 95%ci: 51.7-60.2%) and a ban on all additives (61.7%; 57.5-65.8%), with no significant differences across sociodemographic groups in adjusted logistic regression models.More than half of menthol smokers reported they would either quit or reduce the amount they smoked if menthol cigarettes were banned.Findings suggest that there is support for brazil's ban on flavour additives, which is a determinant of successful policy implemented.Continued delays will postpone an important measure with demonstrated public health gains.

Journal: Preventive medicine (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Exploiting motherhood: Do mummy drinking sites offer real support or are they mainly alcohol marketing?

Even as women's roles have expanded substantially beyond traditional sex stereotypes, women are still commonly portrayed as uncomplaining caregivers, long-suffering intimate partners and in control of family matters, all while maintaining a sexualised femininity.Nowhere are these stereotypes and expectations more apparent than for mothers.However, some social media are exploiting mothers by inappropriately offering alcohol consumption as a solution to the challenges of parenting.This is a very timely topic, given the impacts of covid-19 on family and home life, and potential for an increase in alcohol-related problems and health harms.We address these issues and offer alternatives to alcohol consumption as an easy solution to countering challenges of parenthood.

Journal: Drug and alcohol review (2022)
Publication types: Journal Article
Design strategy and interface chemistry of ageing stable AZO films as high quality transparent conducting oxide.

In this work, an attempt has been made to produce high quality al-doped zn o (azo) transparent conductors by pulsed direct current (dc) magnetron sputtering under varied process conditions, namely sputtering power, sputtering pressure, doping level, and deposition time.The idea is to develop stable azo films with excellent electro-optical properties, which can replace indium tin oxide (ito) as transparent conducting oxide.The films are deposited on glass and polyethylene terephthalate (pet) substrates and characterized by x-ray diffraction (xrd), raman spectroscopy, field emission scanning electron microscopy (fesem), and energy dispersive x-ray spectroscopy (eds) for material properties.The electronic and optical properties are investigated using uv-vis-nir absorption spectroscopy, photoluminescence spectroscopy, four probe resistivity and hall measurements, which facilitate the design strategy to achieve promising electro-optical properties.Azo films under optimized process conditions possess sheet resistance below 5 ω/sq.And maximum visible light transmittance close to 90%.The lowest value of electrical resistivity is found to be 2.1 × 10-4ω·cm.The combination of low electrical resistivity and high optical transparency leading to the figure of merit of the order of 10-2ω-1is critically dependent on the process conditions of sputtering as well as doping contents.High free carrier concentration in azo films leads to the burstein-moss shift and this observed blue shift of the absorption spectrum as compared to that of zn o with native defects depends on the doping contents and the process conditions.This work further explores the role of interface chemistry to achieve high stability of azo films under ambient oxidizing conditions.The adsorption kinetics of cationic rhodamine 6g and anionic methyl orange at azo surface with varying adsorption sites and ageing studies on the sheet resistance reveal that the excellent stability of azo films is delicately linked to the process conditions as well as the content of the dopant material used in the preparation of the azo sputtering targets.The findings of the study confirm that the sheet resistances of selective azo films undergo no change and fixed at 4.5 ω/sq.Even for ageing period of over six months.

Journal: Journal of colloid and interface science (2021)
Publication types: Journal Article
Midline Skull Base Meningiomas: Transcranial and Endonasal Perspectives.

Skull base meningiomas have always represented a challenge for neurosurgeons.Despite their histological nature, they may be associated with unfavorable outcomes due to their deep-seated location and the surrounding neurovascular structures.The state of the art of skull base meningiomas accounts for both transcranial, or high, and endonasal, or low, routes.A comprehensive review of the pertinent literature was performed to address the surgical strategies and outcomes of skull base meningioma patients treated through a transcranial approach, an endoscopic endonasal approach (eea), or both.Three databases (pub med, ovid medline, and ovid embase) have been searched.The review of the literature provided 328 papers reporting the surgical, oncological, and clinical results of different approaches for the treatment of skull base meningiomas.The most suitable surgical corridors for olfactory groove, tuberculum sellae, clival and petroclival and cavernous sinus meningiomas have been analyzed.The eea was proven to be associated with a lower extent of resection rates and better clinical outcomes compared with transcranial corridors, offering the possibility of achieving the so-called maximal safe resection.

Journal: Cancers (2022)
Publication types: Journal Article;Review