syedzayyan/paper_mill_benchmark huggingface dataset:
Low in-hospital mortality rate in patients with COVID-19 receiving thromboprophylaxis: data from the multicentre observational START-COVID Register.

Covid-19 infection causes respiratory pathology with severe interstitial pneumonia and extra-pulmonary complications; in particular, it may predispose to thromboembolic disease.The current guidelines recommend the use of thromboprophylaxis in patients with covid-19, however, the optimal heparin dosage treatment is not well-established.We conducted a multicentre, italian, retrospective, observational study on covid-19 patients admitted to ordinary wards, to describe clinical characteristic of patients at admission, bleeding and thrombotic events occurring during hospital stay.The strategies used for thromboprophylaxis and its role on patient outcome were, also, described.1091 patients hospitalized were included in the start-covid-19 register.During hospital stay, 769 (70.7%) patients were treated with antithrombotic drugs: low molecular weight heparin (the great majority enoxaparin), fondaparinux, or unfractioned heparin.These patients were more frequently affected by comorbidities, such as hypertension, atrial fibrillation, previous thromboembolism, neurological disease, and cancer with respect to patients who did not receive thromboprophylaxis.During hospital stay, 1.2% patients had a major bleeding event.All patients were treated with antithrombotic drugs; 5.4%, had venous thromboembolism [30.5% deep vein thrombosis (dvt), 66.1% pulmonary embolism (pe), and 3.4% patients had dvt + pe].In our cohort the mortality rate was 18.3%.Heparin use was independently associated with survival in patients aged ≥ 59 years at multivariable analysis.We confirmed the high mortality rate of covid-19 in hospitalized patients in ordinary wards.Treatment with antithrombotic drugs is significantly associated with a reduction of mortality rates especially in patients older than 59 years.

Journal: Internal and emergency medicine (2022)
Publication types: Journal Article;Multicenter Study;Observational Study;Research Support, Non-U.S. Gov't
Construction and Analysis of an Enzyme-Constrained Metabolic Model ofCorynebacterium glutamicum.

The genome-scale metabolic model (gem) is a powerful tool for interpreting and predicting cellular phenotypes under various environmental and genetic perturbations.However, gem only considers stoichiometric constraints, and the simulated growth and product yield values will show a monotonic linear increase with increasing substrate uptake rate, which deviates from the experimentally measured values.Recently, the integration of enzymatic constraints into stoichiometry-based gems was proven to be effective in making novel discoveries and predicting new engineering targets.Here, we present the first genome-scale enzyme-constrained model (ec cgl1) for corynebacterium glutamicumreconstructed by integrating enzyme kinetic data from various sources using a ecmpy workflow based on the high-quality gem of c.Glutamicum(obtained by modifying the i cw773 model).The enzyme-constrained model improved the prediction of phenotypes and simulated overflow metabolism, while also recapitulating the trade-off between biomass yield and enzyme usage efficiency.Finally, we used the ec cgl1 to identify several gene modification targets for l-lysine production, most of which agree with previously reported genes.This study shows that incorporating enzyme kinetic information into the gem enhances the cellular phenotypes prediction of c.Glutamicum, which can help identify key enzymes and thus provide reliable guidance for metabolic engineering.

Journal: Biomolecules (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Efficacy of Intravenous Mesenchymal Stem Cells for Motor Recovery After Ischemic Stroke: A Neuroimaging Study.

And purpose stem cell-based therapy is a promising approach to repair brain damage after stroke.This study was conducted to investigate changes in neuroimaging measures using stem cell-based therapy in patients with ischemic stroke.Methods in this prospective, open-label, randomized controlled trial with blinded outcome evaluation, patients with severe middle cerebral artery territory infarct were assigned to the autologous mesenchymal stem cell (msc) treatment or control group.Of 54 patients who completed the intervention, 31 for the msc and 13 for the control groups were included in this neuroimaging analysis.Motor function was assessed before the intervention and 90 days after randomization using the fugl-meyer assessment scale.Neuroimaging measures included fractional anisotropy values of the corticospinal tract and posterior limb of the internal capsule from diffusion tensor magnetic resonance imaging and strength of connectivity, efficiency, and density of the motor network from resting-state functional magnetic resonance imaging.Results for motor function, the improvement ratio of the fugl-meyer assessment score was significantly higher in the msc group compared with the control group.In neuroimaging, corticospinal tract and posterior limb of the internal capsule fractional anisotropy did not decrease in the msc group but significantly decreased at 90 days after randomization in the control group.Interhemispheric connectivity and ipsilesional connectivity significantly increased in the msc group.Change in interhemispheric connectivity showed a significant group difference.Conclusions stem cell-based therapy can protect corticospinal tract against degeneration and enhance positive changes in network reorganization to facilitate motor recovery after stroke.Registration: url: unique identifier: nct01716481.

Journal: Stroke (2022)
Publication types: Journal Article;Randomized Controlled Trial;Research Support, Non-U.S. Gov't
Ten-Year Risk-Prediction Equations for Incident Heart Failure Hospitalizations in Chronic Kidney Disease: Findings from the Chronic Renal Insufficiency Cohort Study and the Multi-Ethnic Study of Atherosclerosis.

Heart failure (hf) is a leading contributor to cardiovascular morbidity and mortality in the population with chronic kidney disease (ckd).Hf risk prediction tools that use readily available clinical parameters to risk-stratify individuals with ckd are needed.Methods we included black and white participants aged 30-79 years with ckd stages 2-4 who were enrolled in the chronic renal insufficiency cohort (cric) study and were without self-reported cardiovascular disease.We assessed model performance of the pooled cohort equations to prevent heart failure (pcp-hf) to predict incident hospitalizations due to hf and refit the pcp-hf in the population with ckd by using cric data-derived coefficients and survival from cric study participants in the ckd population (pcp-hfckd).We investigated the improvement in hf prediction with inclusion of estimated glomerular filtration rate (e gfr) and urine albumin-to-creatinine ratio (uacr) into the pcp-hfckdequations by change in c-statistic, net reclassification improvement (nri), and integrated discrimination improvement index (idi).We validated the pcp-hfckdwith and without e gfr and uacr in multi-ethnic study of atherosclerosis (mesa) participants with ckd.Results among 2328 cric study participants, 340 incident hf hospitalizations occurred over a mean follow-up of 9.5 years.The pcp-hf equations did not perform well in most participants with ckd and had inadequate discrimination and insufficient calibration (c-statistic 0.64-0.71, greenwood-nam-d'agostino (gnd) chi-square statistic p value 0.05).Addition of uacr, but not e gfr, to the refit pcp-hfckdimproved model performance in all race-sex groups (c-statistic [0.73-0.81], gnd chi-square statistic p value > 0.05, delta c-statistic ranging from 0.03-0.11 and nri and idi p values < 0.01).External validation of the pcp-hfckdin mesa demonstrated good discrimination and calibration.Conclusions routinely available clinical data that include uacr in patients with ckd can reliably identify individuals at risk of hf hospitalizations.

Journal: Journal of cardiac failure (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural
Otology Training in India: Is It Up to the Mark?

Different studies have shown that a significant number of medical graduates do not achieve the necessary preparedness for delivery of effective health care in a safe and acceptable manner.Various studies have been undertaken to explore the competencies of surgical residents in otorhinolaryngology.This study was carried out to find out the status of training in otology being provided across different institutions in india.This is a cross sectional study undertaken at all india institute of medical sciences (aiims), jodhpur.An online survey questionnaire enquiring about different aspects of otologic training being followed at various centres was developed and was sent online to all prospective responders.Out of the 217 responders, 88 were faculty members and 129 residents.62% of the faculty members and 56.5% of residents mentioned that their centre has a temporal bone dissection lab.64.7% of the faculty members and 58.2% of the residents responded that temporal bone dissection is mandatory before live surgeries.44% residents have their thesis topic related to otology.About 79% of faculty members and residents are not happy with the the training module being followed in their centre and feel it needs improvement.There needs to be a standardized curriculum that is followed all over the country for teaching the residents during their residency.Increase in existing centers with well equipped staff and facilites is required to provide the residents with opportunities to hone and refine their surgical skills during their training period.Supplementary information the online version contains supplementary material available at ().

Journal: Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India (2022)
Publication types: Journal Article
Relationship between patient sex and anatomical sites of extrapulmonary tuberculosis in Mali.

Contribution of host factors in mediating susceptibility to extrapulmonary tuberculosis is not well understood.Objective to examine the influence of patient sex on anatomical localization of extrapulmonary tuberculosis.Methods we conducted a retrospective cross-sectional study in mali, west africa.Hospital records of 1,304 suspected cases of extrapulmonary tuberculosis, available in tb registry of a tertiary tuberculosis referral center from 2019 to 2021, were examined.Results a total of 1,012 (77.6%) were confirmed to have extrapulmonary tuberculosis with a male to female ratio of 1.59:1.Four clinical forms of eptb predominated, namely pleural (40.4%), osteoarticular (29.8%), lymph node (12.5%), and abdominal tb (10.3%).We found sex-based differences in anatomical localization of extrapulmonary tuberculosis, with males more likely than females to have pleural tb (or: 1.51; 95% ci [1.16 to 1.98]).Conversely, being male was associated with 43% and 41% lower odds of having lymph node and abdominal tb, respectively (or: 0.57 and 0.59).Conclusion anatomical sites of extrapulmonary tuberculosis differ by sex with pleural tb being associated with male sex while lymph node and abdominal tb are predominately associated with female sex.Future studies are warranted to understand the role of sex in mediating anatomical site preference of tuberculosis.

Journal: Journal of clinical tuberculosis and other mycobacterial diseases (2023)
Publication types: Journal Article
Research on the effects of family rituals on subjective well-being of Chinese college students.

Faced with multiple pressures from family, study, employment and interpersonal relationship management, college students are more likely to suffer from mental health problems.At present, psychological intervention in china mainly focuses on drugs and interviews, ignoring the important role played by the family as a bio-psycho-social unit, and there are certain cultural compatibility differences.As an important activity in family life, family rituals have been widely used in the treatment of diseases or mental health in western countries.In contrast, in china, the public's attention and application of family rituals are obviously insufficient, and the relevant academic research results are relatively rare.In view of this, this paper adopts mathematical statistics method to clarify the internal relationship between family rituals and subjective well-being of college students, and verify the mediating role of family system in it, so as to provide effective suggestions for psychological health intervention of college students.The results showed that: family rituals, family system and subjective well-being are correlated in pairs, showing a significant positive correlation; family rituals and family system have significant predictive effects on subjective well-being of college students; the cohesion and adaptability play part of mediating roles between college students' family rituals and subjective well-being.

Journal: Current psychology (New Brunswick, N.J.) (2022)
Publication types: Journal Article
Incidence and risk factor analysis of complications after sentinel node biopsy for penile cancer.

To determine the incidence and types of complications after dynamic sentinel node biopsy (dsnb) in patients with penile cancer (pe ca) and identify risk factors for the occurrence of postoperative complications.Patients and methods we evaluated 644 patients with pe ca (1284 dsnb procedures) with at least one clinically node negative (c n0) groin who underwent dsnb between 2011 and 2020 at a single high-volume centre.The 30- and 30-90-day postoperative complications were collected according to the modified clavien-dindo classification and the standardised methodology proposed by the european association of urology panel.Uni- and multivariable generalised linear mixed models were used to identify risk factors for the occurrence of complications per groin.Results a 30-day postoperative complication occurred in 14% of groins (n = 186), of which 94% were mild to moderate.Wound infection and lymphocele formation were most common.A 30-90-day postoperative complication occurred in 3.4% of the groins, all of which were mild or moderate (grade i-ii).The number of removed lymph nodes (lns) per groin was the main independent predictor for any 30-day complications and grade ≥ii complications (odds ratio 1.40; p < 0.001).There was an increase in the probability of postoperative complications with the number of lns removed after accounting for all confounders.Conclusions despite being less morbid than (modified) inguinal ln dissection, dsnb is still associated with a considerable risk of postoperative mild-to-moderate complications.This risk increases with increasing number of lns removed.Further procedural refinement aimed at removing the true sentinel node(s) only, may help further reduce the morbidity of surgical staging in pe ca.

Journal: BJU international (2022)
Publication types: Journal Article
Circular RNA hsa_circ_0003823 promotes the Tumor Progression, Metastasis and Apatinib Resistance of Esophageal Squamous Cell Carcinoma by miR-607/CRISP3 Axis.

Circular rnas (circ rnas) have attracted a growing interest of research in cancer.The regulatory roles and mechanisms of circ rnas in progression, metastasis and drug resistance of esophageal squamous cell carcinoma (escc) needed to be clarified.Our previous study revealed the crucial role of apatinib in escc therapy.However, the correlation between circ rnas and apatinib resistance remained unclear.methods:3 pairs of tumor and paracancerous tissues of escc patients were used for rna sequencing.Western blot analysis, rna immunoprecipitation (rip), dual-luciferase reporter assays, apoptosis and animal assays were conducted to confirm the roles and specific mechanisms of hsa_circ_0003823 as well as the effects of it on apatinib sensitivity in escc.results:our results revealed that hsa_circ_0003823 was highly expressed in escc and associated with poor prognosis.Further results indicated that hsa_circ_0003823 promoted proliferation and metastasis ability of escc.In the section of mechanism experiments, hsa_circ_0003823 regulated crisp3 by targeting micro rna-607 (mi r-607) to promote progression of escc.Besides, we found that silencing hsa_circ_0003823 improved apatinib sensitivity.Hsa_circ_0003823 resulted in apatinib resistance by mi r-607/crisp3 axis.conclusions:in this study, we elucidated the function of hsa_circ_0003823 and its role in promoting tumor progression, metastasis and apatinib resistance of escc through mi r-607/crisp3 axis.

Journal: International journal of biological sciences (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
RGS1 serves as an antitumor target to inhibit proliferation of NICN87-DR cells and tumor growth in the gastric cancer mouse model.

Gastric cancer is becoming the 4th leading cause of cancer-associated death worldwide.The purpose of this study was to investigate the role of rgs1 in gastric cancer in vitro and in vivo.Proliferation, migration, invasion, and colony formation of ncin87 cells and drug-resistant ncin87 cells (ncin87-dr) were determined.Cell apoptosis and cell cycle were examined using a flow cytometry assay.Rgs1 gene knock-down vector (p lvshsh rgs1) and xenograft tumor mouse model was generated.Rgs1 and epithelial-mesenchymal transition (emt) associated markers, including e-cadherin (e-cad), n-cadherin (n-cad), slug, and vimentin were detected using a western blotting assay.Tumor size of xenograft tumor mouse was measured and ki67 expression was detected using the immunohistochemical assay.Ncin87-dr cells demonstrated significantly lower proliferation, migration, and invasion compared to those of ncin87 cells (p < 0.05).Ncin87-dr cells showed obvious early apoptosis and displayed obvious alterations for the cell cycle.Ncin87-dr cells exhibited predominantly higher rgs1 expression than that in ncin87 cells (p < 0.01).E-cad expression was markedly decreased (p < 0.01) and n-cad (p < 0.05), slug (p < 0.01), vimentin (p < 0.05) expressions were significantly increased in ncin87-dr cells than those in ncin87 cells.Rgs1 gene silence remarkably reduced ncin87-dr proliferation compared to that in ncin87-dr cells without treatment (p < 0.01).Rgs1 gene-silenced ncin87-dr cell immunization predominantly inhibited tumor growth in xenograft tumor mouse than that without rgs1 silence (p < 0.05).Rgs1 gene-silenced ncin87-dr cell immunization significantly downregulated ki67 expression in tumor tissues compared with that without rgs1 silence.In conclusion, rgs1 gene silence reduced the proliferation of ncin87-dr cells in vitro and inhibited tumor growth in vivo.Therefore, rgs1 served as an antitumor target for the gastric cancer treatment.

Journal: Turkish journal of biology = Turk biyoloji dergisi (2022)
Publication types: Journal Article
Emerging roles of long noncoding RNAs in chemoresistance of pancreatic cancer.

Pancreatic cancer is one of the most common causes of cancer death in the world due to the lack of early symptoms, metastasis occurrence and chemoresistance.Therefore, early diagnosis by detection of biomarkers, blockade of metastasis, and overcoming chemoresistance are the effective strategies to improve the survival of pancreatic cancer patients.Accumulating evidence has revealed that long noncoding rna (lnc rna) and circular rnas (circ rnas) play essential roles in modulating chemosensitivity in pancreatic cancer.In this review article, we will summarize the role of lnc rnas in drug resistance of pancreatic cancer cells, including hottip, hotair, pvt1, linc-ror, gas5, uca1, dync2h1-4, meg3, tug1, host2, hcp5, slc7a11-as1 and casc2.We also highlight the function of circ rnas, such as circ hipk3 and circ_0000284, in regulation of drug sensitivity of pancreatic cancer cells.Moreover, we describe a number of compounds, including curcumin, genistein, resveratrol, quercetin, and salinomycin, which may modulate the expression of lnc rnas and enhance chemosensitivity in pancreatic cancers.Therefore, targeting specific lnc rnas and cicr rnas could contribute to reverse chemoresistance of pancreatic cancer cells.We hope this review might stimulate the studies of lnc rnas and cicr rnas, and develop the new therapeutic strategy via modulating these noncoding rnas to promote chemosensitivity of pancreatic cancer cells.

Journal: Seminars in cancer biology (2022)
Publication types: Journal Article;Review;Research Support, Non-U.S. Gov't
Interprofessional student-led clinics: the volunteer patient experience.

Learning from patients and gaining an understanding of their lived experience plays an important role in improving health professions education.However, opportunities for students to engage in interprofessional learning activities involving patients as partners remain limited.In 2018, we developed an interprofessional student-led clinic where people living with parkinson's disease voluntarily participated as 'patient-partners'.The aim of this pilot study was to explore patients' experience and motivation for participation.Methods in 2018 the clinic was implemented five times.Four patient volunteers and six to eight students from a mix of disciplines attended each clinic.Qualitative data were collected via semi-structured focus groups with patients.Data were analysed using thematic analysis.Results eleven patients participated in the focus groups.Patients found the interprofessional nature of the clinic beneficial to their health goals.Their interactions with students from different disciplines helped to build their healthcare knowledge and confidence to ask additional questions of health professionals.Patients felt they offered unique perspectives to students of their own lived experiences.They found sharing their stories with students and each other built a sense of community.Conclusion patients felt they enriched the learning environment, helping students to build their knowledge and skills by providing authentic patient perspectives.The interprofessional aspect enhanced the patient experience in a number of ways.Patients found the multiple perspectives of healthcare helped them to build their own knowledge, and reflect on their changing needs.Warranting further investigation, our findings indicate that participation in the clinics may have positively influenced patients' health seeking behaviours.

Journal: BMC medical education (2022)
Publication types: Journal Article