syedzayyan/paper_mill_benchmark huggingface dataset:
The Antimicrobial Bifunctional Camel Lactoferrin: In Silico and Molecular Dynamic Perspective

Lactoferrin (lf) is a major natural antimicrobial agent secreted in body fluids as a natural innate immunity protein.The action and structure of lf are closely related to its iron‐binding capacity with structural reporting in open and closed conformations.This study looked at how lactoferrin structures change in camel (c lf), bovine (b lf), and human (h lf) lactoferrin closed forms after iron is removed from their binding sites.Initially, the sequence comparison between c lf and the lfs of marine mammals, bats, and domestic animals was the most intriguing conclusion.Camel lf is revealed to be more closely related to marine animals (~80.36% identity) and bats (~79.3% identity) than to terrestrial mammal species (~75.5% identity).Results indicated that c lf was more dynamic in nature than b lf and h lf by showing higher rmsd values.The c lf is known to be half lactoferrin half transferrin; in this study, we show that there are different md behavior of both iron‐binding sites.While lf contains two lobes (c‐ and n‐lobes), the c‐lobe showed high fluctuations as n‐lobe was more stable in the absence of ferric ions.The c‐lobe and n‐lobe of c lf react differently at physiological p h, revealing distinct molecular interactions between these components.In addition, c lf showed higher system flexibility derived from its larger rmsd, rmsf, lower intermolecular hydrogen bonds, and higher solvent accessible surface area (sasa).

Journal: BioMed Research International (2023)
Publication types: Research Article;
The Anti-inflammatory Effect of Chitosan Oligosaccharide on Heart Failure in Mice

Heart failure is currently one of the leading causes of death worldwide, and the inflammatory factors play an important role in its development.Chitosan oligosaccharide (cos), a low‐molecular‐weight form of chitosan, has many specific biological activities.In this study, cos effects on heart failure were studied for the first time by performing transverse arch constriction (tac) surgery in mice, as an animal model of heart failure.Our findings revealed that cos administration (in both 40 mg/kg and 80 mg/kg doses) significantly ameliorated tca‐induced left ventricular (lv) hypertrophy as well as the increase in lung and heart weight in mice, while improving tac‐induced lv dysfunction.Both doses effectively attenuated lv cardiomyocyte hypertrophy, while decreasing heart inflammation after heart failure in mice.In conclusion, our results indicated that the supplementation of cos in normal diet might be an effective way to prevent further myocardial tissue damage in patients suffering from heart failure.

Journal: BioMed Research International (2022)
Publication types: Research Article;
A Novel Artificial Neural Network-Based Biomechanical Research on Elbow Injury of Tennis Serve

Tennis is a sport with a large age span.Its technology is complex.Factors such as tennis racket and service position will affect the effect of players holding the ball, but often the service posture and strength will affect the players’ elbow tendon.Once the service posture is wrong or the strength is too strong, it will cause irreversible damage to players.Therefore, the study of artificial neural network training on tennis elbow injury is of great significance.Based on artificial neural network, this paper studies the biomechanics of elbow injury in tennis service.Firstly, this paper expounds the concept and characteristics of tennis service technology and studies the injury of tennis players’ elbow.Then this paper studies the artificial neural network technology and uses the experimental comparison method to test the force generated by the two groups of tennis players from different angles.The results show that, in the professional group, the forearm is in the internal rotation state before and after hitting the ball, while the beginner group is generally in the external rotation state.It can be inferred that, under the constant collision force, the reversal force absorbed by the beginner group in the swing process is greater than that of the professional group, which will increase the load of the supinator muscle on the antipronation, which will damage the supinator muscle, which is also one of the causes of the “tennis elbow” disease.

Journal: Security and Communication Networks (2022)
Publication types: Research Article;
Targeting Streptomyces-Derived Streptenol Derivatives against Gynecological Cancer Target PIK3CA: An In Silico Approach

Streptomyces is amongst the most amenable genera for biotechnological applications, and it is extensively used as a scaffold for drug development.One of the most effective therapeutic applications in the treatment of cancer is targeted therapy.Small molecule therapy is one of them, and it has gotten a lot of attention recently.Streptomyces derived compounds namely streptenols a, c, and f–i and streptazolin were subjected for admet property assessment.Our computational studies based on molecular docking effectively displayed the synergistic effect of streptomyces‐derived compounds on the gynecological cancer target pik3ca.These compounds were observed with the highest docking scores as well as promising intermolecular interaction stability throughout the molecular dynamic simulation.Molecular docking and molecular dynamic modeling techniques were utilized to investigate the binding mode stability of drugs using a pharmacophore scaffold, as well as physicochemical and pharmacokinetic aspects linked to alpelisib.With a root mean square fluctuation of the protein backbone of less than 0.7 nm, they demonstrated a steady binding mode in the target binding pocket.They have also prompted hydrogen bonding throughout the simulations, implying that the chemicals have firmly occupied the active site.A comprehensive study showed that streptenol d, streptenol e, streptenol c, streptenol g, streptenol f, and streptenol b can be considered as lead compounds for pik3ca‐based inhibitor design.To warrant the treatment efficacy against cancer, comprehensive computational research based on proposed chemicals must be assessed through in vitro studies.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Systematic Understanding of the Mechanism of Baicalin against Gastric Cancer Using Transcriptome Analysis

Gastric cancer (gc) is the most common type of cancer.It is highly malignant and is characterized by rapid and uncontrolled growth.The antitumour activity of baicalin was studied in multiple cancers.However, its mechanism of action has not been fully elucidated.We provided a systematic understanding of the mechanism of action of baicalin against gc using a transcriptome analysis of rna‐seq.Methods .Human gc cells (sgc‐7901) were exposed to 200 μ g/ml baicalin for 24 h.Rna‐seq with a transcriptome, gene ontology (go), and kyoto encyclopedia of genes and genomes (kegg) enrichment analyses were used to identify the antitumour effects of baicalin on sgc‐7901 cells in vitro.A protein‐protein interaction (ppi) network of differentially expressed genes (degs) was constructed.A competitive endogenous rna (ce rna) network was constructed and further analysed after validation using q rt‐pcr.Results .A total of 68 lnc rnas, 20 mi rnas, and 1648 m rnas were differentially expressed in baicalin‐treated sgc‐7901 gc cells.Three lnc rnas, 6 mi rnas, and 7 m rnas were included in the ce rna regulatory network.Go analysis revealed that the main degs were involved in the biological processes of the cell cycle and cell death.Kegg pathway analysis further suggested that the p53 signalling pathway was involved in the baicalin‐induced antitumour effect on sgc‐7901 cells.Further confirmation using q pcr indicated that baicalin induced an antitumour effect on sgc‐7901 cells, which is consistent with the results of the sequencing data.Conclusions .In summary, the mechanism of baicalin against gc involves multiple targets and signalling pathways.These results provide new insight into the antitumour mechanism of baicalin and help the development of new strategies to cure gc.

Journal: BioMed Research International (2021)
Publication types: Research Article;
A Multichannel Model for Microbial Key Event Extraction Based on Feature Fusion and Attention Mechanism

In order to further mine the deep semantic information of the microbial text of public health emergencies, this paper proposes a multichannel microbial sentiment analysis model mcmf-a.Firstly, we use word2vec and fast text to generate word vectors in the feature vector embedding layer and fuse them with lexical and location feature vectors; secondly, we build a multichannel layer based on cnn and bi lstm to extract local and global features of the microbial text; then we build an attention mechanism layer to extract the important semantic features of the microbial text; thirdly, we merge the multichannel output in the fusion layer and use soft; finally, the results are merged in the fusion layer, and a surtax function is used in the output layer for sentiment classification.The results show that the f1 value of the mcmf-a sentiment analysis model reaches 90.21%, which is 9.71% and 9.14% higher than the benchmark cnn and bi lstm models, respectively.The constructed dataset is small in size, and the multimodal information such as images and speech has not been considered.

Journal: Security and Communication Networks (2021)
Publication types: Research Article;
Synthesis and Evaluation of Finasteride-Loaded HPMC-Based Nanogels for Transdermal Delivery: A Versatile Nanoscopic Platform

Herein, we report nanogels comprising diverse feed ratio of polymer hydroxypropyl methylcellulose (hpmc), monomer acrylic acid (aa), and cross‐linker methylene bisacrylamide (mba) fabricated for transdermal delivery of finasteride (fin).Free radical solution polymerization method with subsequent condensation was employed for the synthesis using ammonium per sulfate (aps) and sodium hydrogen sulfite (shs) as initiators.Carbopol‐940 gel (cg) was formulated as assisting platform to deliver fin nanogels transdermally.Developed formulations were evaluated by several in vitro , ex vivo , and in vivo parameters such as particle size and charge distribution analysis, fourier transform infrared spectroscopy (ftir), thermogravimetric analysis (tga), differential scanning calorimetry (dsc), scanning electron microscopy (sem), x‐ray diffractogram (xrd), rheological testing, in vitro swelling and drug release, and ex vivo skin permeation, irritation, and toxicity assessment.The results endorsed the nanogel formation (117.3 ± 29.113 nm), and the impact of synthesizing method was signified by high yield of nanogels (≈91%).Efficient response for in vitro swelling and fin release was revealed at p h 5.5 and 7.4.Skin irritation and toxicity assessment ensured the biocompatibility of prepared nanocomposites.On the basis of the results obtained, it can be concluded that the developed nanogels were stable with excellent drug permeation profile across skin.

Journal: BioMed Research International (2022)
Publication types: Research Article;
Synthesis and Biomedical Applications of Zirconium Nanoparticles: Advanced Leaps and Bounds in the Recent Past

Many synthetic routes manufacture zirconium nanoparticles in metal oxide, nitride, and other combination forms.Coupled with other variables such as concentration, p h, and form of precursor used, the various synthetic methods support synthesizing the zirconium metal oxide nanoparticles with changed features.Various synthetic methods were studied, such as sol‐gel, hydrothermal, laser ablation, and precipitation.All have different synthetic routes, different precursors and solvents were sued, and the product was characterized by sem, tem, photo luminance spectroscopy, uv‐absorption spectroscopy, and powder x‐ray diffraction.X‐ray diffraction determined the crystal structure by identifying the crystal shape, arrangement of atoms, and spacing between them.Sem and tem studied the particle size and morphology of nanoparticles.Uv‐visible absorption spectroscopy and pl spectroscopy were used for the determination of optical properties of nanoparticles.Zirconium oxide nanoparticles have many applications in the medical field.The review study primarily focuses on the efficient combination of zirconium dioxide with other additive materials and functionalization techniques used to improve the material’s properties, assisting the use of the material in hip arthroplasty and bone tissue applications.The development of sophisticated near‐infrared (nir) absorbing small molecules for useful phototheranostic applications was discussed in this paper.

Journal: BioMed Research International (2022)
Publication types: Review Article;
Taurine Ameliorates Iron Overload-Induced Hepatocyte Injury via the Bcl-2/VDAC1-Mediated Mitochondrial Apoptosis Pathway

Iron overload can induce reactive oxygen species (ros) burst and liver damage.Taurine can reduce ros production and ameliorate liver injury caused by iron overload; however, the underlying molecular mechanism remains elusive.Herein, l02 cells treated with 120 μm iron dextran for 48 h showed marked oxidative stress damage and significantly increased apoptosis.Taurine protected hepatocytes by stabilizing mitochondrial membranes and resisting oxidative stress damage caused by iron overload.However, transfection with si rna bcl-2 virus abrogated the observed protective effects.Following treatment with taurine, b cell lymphoma-2 (bcl-2) could inhibit the opening of the mitochondrial permeability transition pore (m ptp), subsequently stabilizing the mitochondrial membrane potential by interacting with voltage-dependent anion channel 1 (vdac1) of m ptp.The present study is the first to clarify the mechanism underlying taurine-afforded hepatocyte protection against iron overload-induced oxidative stress via bcl-2-mediated inhibition of m ptp opening and the antiapoptotic pathway.

Journal: Oxidative Medicine and Cellular Longevity (2022)
Publication types: Research Article;
Target Characteristics and Voltage Mapping of the Matrix in Idiopathic Premature Ventricular Contractions Originating from the Right Ventricular Outflow Tract

The study was aimed at exploring the electrophysiological characteristics (eps) of the optimal ablation site and its relationship with electroanatomic voltage mapping (evm) in idiopathic premature ventricular contractions (pvcs) originating from the right ventricular outflow tract (rvot).Methods .A total of 28 patients with idiopathic rvot pvcs underwent successful ablation and evm using a 3d electroanatomical mapping (carto) system.Results .Both bipolar and unipolar evm showed a similar band‐like lower‐voltage area (lva) under the pulmonary valve in all the patients; 21.4% of the targets were located in the band‐like lva.42.9% of the targets were at the border of the band‐like lva on the bipolar voltage map, but unipolar mapping showed that 53.6% of the targets were located in the band‐like lva, and 35.7% of the targets at the border of the band‐like lva.A significant difference was found in both unipolar and bipolar voltage values between the regions within 0‐5 mm above the optimal ablation site and the other regions.A similar difference was observed only in unipolar voltage values below the optimal ablation site.At the ablation site, there were frequent occurrences of a fragmented wave and voltage reversion in the bipolar electrograms, frustrated falling limbs, w bottom, and a qs configuration width &gt; 150 ms in the unipolar electrograms.Conclusions .Evm showed that the band‐like lva was an interesting area for the search of the optimal ablation sites of idiopathic rvot‐pvcs, especially the border area.There was focal microscarring around the ablation targets; some characteristics of eps proved significant for successful ablation.

Journal: BioMed Research International (2021)
Publication types: Research Article;
Systematic Evaluation and Meta-Analysis of Randomized Controlled Trials of Fire Needle Combined Phototherapy for the Treatment of Vitiligo

This study investigated the fire needle and phototherapy combination for treating vitiligo through a meta‐analysis of the published literature.Indeed, vitiligo is a common chronic skin condition characterized by the appearance of white patches.It is the most prevalent disorder, affecting approximately 1% of the global population.There is no known cure or clinical recommendation for treating vitiligo.The majority of medical guidelines suggest vitiligo treatment with the use of fire needles.Here, vitiligo was treated with a novel mechanism combining a fire needle with phototherapy.The handheld phototherapy devices include a fire needle option for disease treatment.Miniature lesions that could be used to detect and treat vitiligo at an early stage could be managed by hybrid‐capable devices.The real‐time study included more than 3,435 patients.The dosages were altered to control the adverse effects.Following treatment, granulation tissues developed on the injured skin, diminishing the shallow area and wound surface.The case studies demonstrate that combining phototherapy and fire needle therapy is more practical than the other methods.

Journal: BioMed Research International (2022)
Publication types: Meta-Analysis;
Synthesis, Characterization, and Specific Localization of Mitochondrial-Targeted Antioxidant Peptide SS31 Probe

The aim of this study is to investigate the targeting efficiency of fitc‐ss31 to mitochondria in both normal and h 2 o 2 ‐induced oxidative damaged 661w cells, characterizing the properties of fitc‐ss31 in the biological assays.The purity and molecular weight of fitc‐ss31 were identified using hplc and ms.Mtt and ldh assays were used to evaluate the cytotoxicity and cell permeability.The binding ability of fitc‐ss31 to cells was demonstrated by flow cytometry.The colocalization of fitc‐ss31 and mito tracker both in normal and oxidative cells was analyzed by a laser confocal microscope.We detected the degs between ss31+h 2 o 2 and h 2 o 2 ‐alone‐treated cells by rna seq.Go and kegg analyses were performed to predict the functional gene of ss31.The molecular weight of fitc‐ss31 was 1142.2 with the 97.76% purity.The flow cytometry results showed that the mfi (mean fluorescence intensity) of fitc‐ss31 in normal cells in the 4 h probe treatment group was higher than that in the 2 h and the 0 h group.The mfi in the 2 h probe treatment group was much higher than that in the 4 h and 0 h groups in damaged cells.The positive rate of 10 μ m fitc‐ss31 was higher than that of 1 μ m and 5 μ m.Fluorescein imaging analysis confirmed that fitc‐ss31 was overlapped with mito tracker.Through the analysis, degs were highly expressed in “localization, organelle, antioxidant activity, binding” functions and enriched in “ampk signaling pathway, mapk targets/nuclear events mediated by map kinase pathway and pi3k‐akt signaling pathway.” it is speculated that ss31 exerts an antioxidant effect through one of these pathways.We hypothesized that ss31 could play a more efficient role in the pathological cells in the half‐life period to avoid cell death due to oxidative damage.The functions of the degs in ss31+h 2 o 2 and h 2 o 2 ‐alone samples are related to the localization and antioxidant activity of ss31.Degs are mostly enriched in the ampk signaling pathway, which needs further studies.

Journal: BioMed Research International (2021)
Publication types: Research Article;
Styrylchromones: Biological Activities and Structure-Activity Relationship

Styrylchromones (sc) are a group of oxygen‐containing heterocyclic compounds, which are characterized by the attachment of a styryl group to the chromone core.Sc can be found in nature or can be chemically synthesized in the laboratory.As their presence in nature is scarce, the synthetic origin is the most common.Two types of sc are known: 2‐styrylchromones and 3‐styrylchromones.However, 2‐styrylchromones are the most common, being more commonly found in nature and which chemical synthesis is more commonly described.A wide variety of sc has been described in the literature, with different substituents in different positions, the majority of which are distributed on the a‐ and/or b‐rings.Over the years, several biological activities have been attributed to sc.This work presents a comprehensive review of the biological activities attributed to sc and their structure‐activity relationship, based on a published literature search, since 1989.The following biological activities are thoroughly revised and discussed in this review: antioxidant, antiallergic, antiviral, antibacterial, antifungal, anti‐inflammatory, and antitumoral, affinity and selectivity for a 3 adenosine receptors, neuroprotective, and α ‐glucosidase inhibition.In general, sc are composed by a promising scaffold with great potential for the development of new drugs.

Journal: Oxidative Medicine and Cellular Longevity (2021)
Publication types: Review Article;
Study on Damage Mechanism and Repair of Vascular Endothelial Cells Based on Ultrasound Technology

To detect the endothelial function of external iliac artery in rats with different stages of atherosclerosis by high‐resolution ultrasound, so as to provide experimental methodological basis for evaluating the function of vascular endothelial cells by ultrasound.Methods .The animals were randomly divided into the control group ( n = 6) and the atherosclerosis model group ( n = 15).The atherosclerosis group was further divided into 4‐week group, 8‐week group, and 12‐week group, with 5 animals in each group.After separating and grinding rat spleen, the obtained cells were cultured by density gradient centrifugation.After the cells adhered, the morphology of the cells was observed under a microscope and identified by di i‐ac‐ldl and fitc‐uea‐i double staining.The activities of ldh and sod, the contents of mda and gsh, and the contents of no in plasma were detected by biochemical methods.Results .The protective effect of rosanilin on brain injury in rats with acute hypobaric hypoxia and its regulation on the expression of p akt protein; ox‐ldl inhibited the proliferation activity of epcs in a concentration‐dependent manner.The expression of klf2 and s1pr1 in haec can be knocked down by small interfering rna, and knocking down klf2 can not only downregulate the expression of s1pr1 but also downregulate haven.With the development of atherosclerosis, the endothelium‐dependent relaxation function and endothelium‐independent relaxation function of the control group and the atherosclerosis model at 4, 8, and 12 weeks were damaged in different degrees and gradually aggravated.Conclusion .Atherosclerosis is a disease with both morphological and functional damage, and vascular endothelial function is damaged in the early stage with corresponding pathological changes.Ultrasound is an effective method to evaluate vascular endothelial function.

Journal: BioMed Research International (2021)
Publication types: Research Article;
Statistical Analysis of Employment Education in Colleges and Universities Based on Improved Clustering Algorithm

In order to improve the effect of employment education in colleges and universities, this study combines the improved clustering algorithm to carry out statistical analysis of employment education in colleges and universities and analyzes the current situation of employment education in colleges and universities.In cluster analysis, considering the complexity of the distribution of statistics after the reestimation of the sample size and the possible complex correlation between the series of statistics in the group sequential design, on the premise that the statistics used will not cause type i error expansion after sample size adjustment, some rules for reestimating the sample size can be formulated.Through the simulation analysis, it can be seen that the statistical analysis system of college employment education based on the improved clustering algorithm proposed in this study has a good effect in the clustering of employment education data and has a certain role in promoting the employment of college graduates.

Journal: Security and Communication Networks (2022)
Publication types: Research Article;