syedzayyan/paper_mill_benchmark huggingface dataset:
The painful shoulder arthroplasty: appropriate work-up and review of interventional pain treatments.

Shoulder arthroplasty is a successful surgical procedure for several conditions when patients become refractory to conservative management modalities.Unfortunately, some patients experience persistent chronic pain after shoulder arthroplasty.These individuals should undergo a comprehensive evaluation by an orthopedic surgeon to determine whether structural pathology is responsible for the pain and to decide whether reoperation is indicated.At times, a surgical solution does not exist.In these circumstances, a thorough and specific plan for the management of persistent chronic pain should be developed and instituted.In this article, we review common reasons for persistent pain after shoulder arthroplasty and outline the evaluation of the painful shoulder arthroplasty.We then provide a thorough review of interventional pain management strategies.Finally, we hypothesize developments in our field that might provide better outcomes in the future for patients suffering with chronic intractable pain after shoulder arthroplasty.

Journal: JSES reviews, reports, and techniques (2022)
Publication types: Journal Article;Review
Directionality of dynein is controlled by the angle and length of its stalk.

The ability of cytoskeletal motors to move unidirectionally along filamentous tracks is central to their role in cargo transport, motility and cell division.Kinesin and myosin motor families have a subclass that moves towards the opposite end of the microtubule or actin filament with respect to the rest of the motor family1,2, whereas all dynein motors that have been studied so far exclusively move towards the minus end of the microtubule3.Guided by cryo-electron microscopy and molecular dynamics simulations, we sought to understand the mechanism that underpins the directionality of dynein by engineering a saccharomyces cerevisiae dynein that is directed towards the plus end of the microtubule.Here, using single-molecule assays, we show that elongation or shortening of the coiled-coil stalk that connects the motor to the microtubule controls the helical directionality of dynein around microtubules.By changing the length and angle of the stalk, we successfully reversed the motility towards the plus end of the microtubule.These modifications act by altering the direction in which the dynein linker swings relative to the microtubule, rather than by reversing the asymmetric unbinding of the motor from the microtubule.Because the length and angle of the dynein stalk are fully conserved among species, our findings provide an explanation for why all dyneins move towards the minus end of the microtubule.

Journal: Nature (2019)
Publication types: Journal Article;Research Support, N.I.H., Extramural;Research Support, Non-U.S. Gov't;Research Support, U.S. Gov't, Non-P.H.S.
Early-life vitamin B12 orchestrates lipid peroxidation to ensure reproductive success via SBP-1/SREBP1 in Caenorhabditis elegans.

Vitamin b12 (b12) deficiency is a critical problem worldwide.Such deficiency in infants has long been known to increase the propensity to develop obesity and diabetes later in life through unclear mechanisms.Here, we establish a caenorhabditis elegans model to study how early-life b12 impacts adult health.We find that early-life b12 deficiency causes increased lipogenesis and lipid peroxidation in adult worms, which in turn induces germline defects through ferroptosis.Mechanistically, we show the central role of the methionine cycle-sbp-1/srebp1-lipogenesis axis in programming adult traits by early-life b12.Moreover, sbp-1/srebp1 participates in a crucial feedback loop with nhr-114/hnf4 to maintain cellular b12 homeostasis.Inhibition of sbp-1/srebp1-lipogenesis signaling and ferroptosis later in life can reverse disorders in adulthood when b12 cannot.Overall, this study provides mechanistic insights into the life-course effects of early-life b12 on the programming of adult health and identifies potential targets for future interventions for adiposity and infertility.

Journal: Cell reports (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
The five times sit-to-stand test: safety, validity and reliability with critical care survivors's at ICU discharge.

The five times sit-to-stand test (ftsst) has been found reliable, safe and valid for measuring healthy adults' lower limb muscle strength and for determining balance control, fall risk, and exercise capacity among older examinees.We believe that the ftsst has the potential to be a straightforward, low cost and valuable tool for identifying muscle disability and functional status following critical illness.The aim of our study was to establish the applicability, safety, and psychometric qualities of ftsst in patients at intensive care unit (icu) discharge.Methods in our study applicability was determined by assessing the percentage of patients who could perform the test at icu discharge.Safety was assessed by examining data regarding any exacerbated haemodynamic and respiratory responses or adverse events associated with the test.For assessing ftsst reliability, intraclass correlation coefficients (iccs), standard error of measurement (sem) and bland-altman plot were used.For assessing concurrent validity handgrip strength, icu length of stay, duration of invasive ventilation, simplified acute physiology score 3 (saps3) and age variables were used.For investigating predictive validity, correlations between the ftsst and measures of hospital length of stay and functional independence were evaluated.Results only 30% of icu survivors (n = 261 out of 817) were eligible to perform the ftsst and 7% of patients who performed the test (n = 10 out of 142) presented adverse events.Both inter (icc 0.92 ci95% 0.89-0.94) and intra-rater (icc 0.95 ci95% 0.93-0.96) reliability were excellent and higher scores were associated with lower muscle strength, longer hospital stay and greater functional impairment at hospital discharge in adult survivors of critical diseases.Conclusion our results suggest that the ftsst may be applicable only to high-functioning critical care survivors.In this specifical population, ftsst is a safe, easy to perform, valid and reliable measure that can be applied to fall risk and functional recovery management.

Journal: Archives of physiotherapy (2022)
Publication types: Journal Article
PARP1-MGMT complex underpins pathway crosstalk in O6-methylguanine repair.

Dna lesions induced by alkylating agents are repaired by two canonical mechanisms, base excision repair dependent on poly(adp) ribose polymerase 1 (parp1) and the other mediated by o6-methylguanine (o6me g)-dna methyltransferase (mgmt) in a single-step catalysis of alkyl-group removal.O6me g is the most cytotoxic and mutagenic lesion among the methyl adducts induced by alkylating agents.Although it can accomplish the dealkylation reaction all by itself as a single protein without associating with other repair proteins, evidence is accumulating that mgmt can form complexes with repair proteins and is highly regulated by a variety of post-translational modifications, such as phosphorylation, ubiquitination, and others.Here, we show that parp1 and mgmt proteins interact directly in a non-catalytic manner, that mgmt is subject to parylation by parp1 after dna damage, and that the o6me g repair is enhanced upon mgmt parylation.We provide the first evidence for the direct dna-independent parp1-mgmt interaction.Further, parp1 and mgmt proteins also interact via parylation of mgmt leading to formation of a novel dna damage inducible parp1-mgmt protein complex.This catalytic interaction activates o6me g repair underpinning the functional crosstalk between base excision and mgmt-mediated dna repair mechanisms.Furthermore, clinically relevant 'chronic' temozolomide exposure induced parylation of mgmt and increased binding of parp1 and mgmt to chromatin in cells.Thus, we provide the first mechanistic description of physical interaction between parp1 and mgmt and their functional cooperation through parylation for activation of o6me g repair.Hence, the parp1-mgmt protein complex could be targeted for the development of advanced and more effective cancer therapeutics, particularly for cancers sensitive to parp1 and mgmt inhibition.

Journal: Journal of hematology & oncology (2022)
Publication types: Letter;Research Support, Non-U.S. Gov't;Research Support, N.I.H., Extramural
Social Support and Acculturative Stress of International Students.

Continuing to study abroad brings challenges, apart from academic demands.International students are prone to acculturation stress as a consequence of cultural differences.Many research reports show that social support is a great buffer against experienced stress, but there has thus far been no study that analyzes the real effect of social support on acculturation stress.This meta-analysis study aims to investigate the true effect of social support on acculturation stress of international students based on studies reporting it.A meta-analysis was performed following prisma.The electronic databases used were science-direct, eric, pro quest, google scholar (only for etd), and opengrey.edu, with the article year limitations being 2009-2019.Eight (8) studies were involved in the meta-analysis.There were three instruments of acculturation stress and five instruments of social support that were used in the different studies.The effect size analysis showed that there was no difference in the effects of eight studies (z = -0.553; se = 0.497; 95% ci = -1.248--0.699;p= 0.580).Furthermore, there was no statistically significant moderator variable, the instruments used were quite diverse.The role of a moderator, other than gender, was not used because of limited information from the studies used.Social support plays a major role in reducing acculturation stress in international students.However, studies involving moderator and confounding roles need to be conducted.

Journal: International journal of environmental research and public health (2022)
Publication types: Journal Article;Meta-Analysis;Review
Paying the Iron Price: Liver Iron Homeostasis and Metabolic Disease.

Iron is an essential metal element whose bioavailability is tightly regulated.Under normal conditions, systemic and cellular iron homeostases are synchronized for optimal function, based on the needs of each system.During metabolic dysfunction, this synchrony is lost, and markers of systemic iron homeostasis are no longer coupled to the iron status of key metabolic organs such as the liver and adipose tissue.The effects of dysmetabolic iron overload syndrome in the liver have been tied to hepatic insulin resistance, nonalcoholic fatty liver disease, and nonalcoholic steatohepatitis.While the existence of a relationship between iron dysregulation and metabolic dysfunction has long been acknowledged, identifying correlative relationships is complicated by the prognostic reliance on systemic measures of iron homeostasis.What is lacking and perhaps more informative is an understanding of how cellular iron homeostasis changes with metabolic dysfunction.This article explores bidirectional relationships between different proteins involved in iron homeostasis and metabolic dysfunction in the liver.© 2022 american physiological society.Compr physiol 12:3641-3663, 2022.

Journal: Comprehensive Physiology (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't;Research Support, U.S. Gov't, Non-P.H.S.;Research Support, N.I.H., Extramural
A Retrospective Study of the Clinical Characteristics of Japanese Encephalitis in Adults.

This study aimed to explore the diagnostic points and treatment modes of the clinical characteristics of japanese encephalitis (je) in the middle-aged and elderly population.Methods six patients aged 47-72 who were diagnosed with je at the beijing chaoyang hospital affiliated with the capital medical university between august 2018 and september 2019 were enrolled in the study.Their clinical manifestations, biochemical indicators, imaging data, diagnostic methods, and the evolution and outcomes of the treatments they underwent were retrospectively analyzed.Results (1) all six patients had severe clinical symptoms and poor prognoses that were more likely to be associated with other systemic diseases.(2) lesions were most commonly distributed in the thalamus, basal ganglia, and midbrain.The appearance of hyperintensity in the corpus callosum, hippocampus, and subcortical white matter was more specific.The hyperperfusion metabolism in the lesion area in head computed tomography perfusion imaging indicated the state of inflammatory activity in the lesion.In cranial magnetic resonance imaging (mri), t2 and fluid-attenuated inversion recovery (flair) were more sensitive.(3) after a patient has been systematically treated in the intensive care unit (icu), the patient gradually recovered and the level of consciousness improved (p< 0.05).Conclusions in brain mri-especially t2 and flair-intracranial infection is often accompanied by abnormal signals in the thalamus, midbrain, hippocampus, and white matter hyperintensity (wmh), which is highly suggestive of je.The positive detection of anti-je virus immunoglobulin m antibodies in a patient's serum and/or cerebrospinal fluid can confirm the diagnosis of je, and comprehensive icu treatment (hormones combined with anti-inflammatory, antiviral, and mild hypothermic cerebral protection therapies) can improve the survival rate.

Journal: Journal of integrative neuroscience (2022)
Publication types: Journal Article
Patient Safety in Anesthesia: Hand Hygiene and Perioperative Infection Control.

Of review this review highlights the importance of the anesthesia team in minimizing perioperative infection risks and prevention of surgical site infection.Due to the immense financial and patient care burden that results from perioperative infection, a foundational knowledge in preventive measures is essential.Recent findings perioperative infection control, the role of the anesthesia team in reducing infection risk, and more specifically the outsized importance of hand hygiene in this space have become increasingly apparent.Maintenance of workspace cleanliness along with hand hygiene forms the cornerstone of preventing microbial transmission.Unfortunately, improvements around perioperative infection control are lacking.Summary the importance of the anesthesia team in maintaining proper hand hygiene, a clean work environment, and appropriate patient conditions to minimize risk of perioperative infection cannot be overstated.Poor clinical outcomes, economic burden, and external pressure from payers highlight the need for anesthesia providers to have an up-to-date knowledge of best practices in this area.In this article, we will review the current recommendations for hand hygiene practices and perioperative infection prevention.

Journal: Current anesthesiology reports (2022)
Publication types: Journal Article;Review
Evaluating agreement between bodies of evidence from randomised controlled trials and cohort studies in nutrition research: meta-epidemiological study.

To evaluate the agreement between diet-disease effect estimates of bodies of evidence from randomised controlled trials and those from cohort studies in nutrition research, and to investigate potential factors for disagreement.Design meta-epidemiological study.Data sources cochrane database of systematic reviews, and medline.Review methods population, intervention or exposure, comparator, outcome (pi/eco) elements from a body of evidence from cohort studies (bo e(cs)) were matched with corresponding elements of a body of evidence from randomised controlled trials (bo e(rct)).Pooled ratio of risk ratios or difference of mean differences across all diet-disease outcome pairs were calculated.Subgroup analyses were conducted to explore factors for disagreement.Heterogeneity was assessed through i2and τ2.Prediction intervals were calculated to assess the range of possible values for the difference in the results between evidence from randomised controlled trials and evidence from cohort studies in future comparisons.Results 97 diet-disease outcome pairs (that is, matched bo e(rct) and bo e(cs)) were identified overall.For binary outcomes, the pooled ratio of risk ratios comparing estimates from bo e(rct) with bo e(cs) was 1.09 (95% confidence interval 1.04 to 1.14; i2=68%; τ2=0.021; 95% prediction interval 0.81 to 1.46).The prediction interval indicated that the difference could be much more substantial, in either direction.We further explored heterogeneity and found that pi/eco dissimilarities, especially for the comparisons of dietary supplements in randomised controlled trials and nutrient status in cohort studies, explained most of the differences.When the type of intake or exposure between both types of evidence was identical, the estimates were similar.For continuous outcomes, small differences were observed between randomised controlled trials and cohort studies.Conclusion on average, the difference in pooled results between estimates from bo e(rct) and bo e(cs) was small.But wide prediction intervals and some substantial statistical heterogeneity in cohort studies indicate that important differences or potential bias in individual comparisons or studies cannot be excluded.Observed differences were mainly driven by dissimilarities in population, intervention or exposure, comparator, and outcome.These findings could help researchers further understand the integration of such evidence into prospective nutrition evidence syntheses and improve evidence based dietary guidelines.

Journal: BMJ (Clinical research ed.) (2021)
Publication types: Journal Article
Results of untargeted analysis using the SOMAscan proteomics platform indicates novel associations of circulating proteins with risk of progression to kidney failure in diabetes.

This study applies a large proteomics panel to search for new circulating biomarkers associated with progression to kidney failure in individuals with diabetic kidney disease.Four independent cohorts encompassing 754 individuals with type 1 and type 2 diabetes and early and late diabetic kidney disease were followed to ascertain progression to kidney failure.During ten years of follow-up, 227 of 754 individuals progressed to kidney failure.Using the somascan proteomics platform, we measured baseline concentration of 1129 circulating proteins.In our previous publications, we analyzed 334 of these proteins that were members of specific candidate pathways involved in diabetic kidney disease and found 35 proteins strongly associated with risk of progression to kidney failure.Here, we examined the remaining 795 proteins using an untargeted approach.Of these remaining proteins, 11 were significantly associated with progression to kidney failure.Biological processes previously reported for these proteins were related to neuron development (dll1, matn2, nrx1b, klk8, rtn4r and ror1) and were implicated in the development of kidney fibrosis (layn, dll1, mapk11, matn2, endostatin, and ror1) in cellular and animal studies.Specific mechanisms that underlie involvement of these proteins in progression of diabetic kidney disease must be further investigated to assess their value as targets for kidney-protective therapies.Using multivariable lasso regression analysis, five proteins (layn, esam, dll1, mapk11 and endostatin) were found independently associated with risk of progression to kidney failure.Thus, our study identified proteins that may be considered as new candidate prognostic biomarkers to predict risk of progression to kidney failure in diabetic kidney disease.Furthermore, three of these proteins (dll1, esam, and mapk11) were selected as candidate biomarkers when all somascan results were evaluated.

Journal: Kidney international (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural;Research Support, Non-U.S. Gov't;Research Support, N.I.H., Intramural
Arginase II Promotes Intervertebral Disc Degeneration Through Exacerbating Senescence and Apoptosis Caused by Oxidative Stress and Inflammationviathe NF-κB Pathway.

Intervertebral disc degeneration (idd) has been generally accepted as the major cause of low back pain (lbp), which imposes massive clinical and socioeconomic burdens.Previous studies have demonstrated that oxidative stress and inflammation-induced senescence and apoptosis of nucleus pulposus cells (npcs) are the main cellular processes that cause idd.Arginase ii (arg2), an enzyme involved in a variety of pathological processes, including cellular senescence, apoptosis, oxidative stress, and inflammation, has been shown to promote degeneration in several degenerative diseases, including osteoarticular diseases.Based on previous studies, we hypothesized that arg2 deficiency might be conducive to the treatment of idd by inhibiting the dyshomeostasis of the extracellular matrix (ecm), and the oxidative stress and inflammatory response-induced senescence and apoptosisvia nf-κb.In this study, we found that arg2 deficiency inhibited senescence and apoptosis of npcs, and degeneration of the ecm induced by oxidative stress and the inflammatory response.Similar results were found with the selective nf-κb pathway inhibitor jsh-23.In contrast, overexpression of arg2 had the opposite effect.Taken together, our results suggest that arg2 deficiency prevents iddvia nf-κb, and may therefore, be a potential therapeutic strategy for idd.

Journal: Frontiers in cell and developmental biology (2021)
Publication types: Journal Article
Short-term impact of aged garlic extract on endothelial function in diabetes: A randomized, double-blind, placebo-controlled trial.

Impaired endothelial function portends an increased risk of cardiovascular disease.Vascular oxidative stress and systemic inflammation play a critical role in the pathogenesis and progression of vascular disease.Aged garlic extract (age) may improve impaired vascular endothelial function, while decreasing the progression of atherosclerotic plaque.We hypothesized that age may improve endothelial function, and in this study, we examined this hypothesis to determine whether this can be achieved over a period of 3 months, measured by the cardio-ankle vascular index (cavi), by reducing intracellular oxidant stress and stimulating nitric oxide generation in endothelial cells.We conducted a double-blinded placebo controlled, randomized clinical trial to investigate the effects of age on cavi in subjects with type 2 diabetes mellitus.A total of 65 individuals (38 men and 27 women) with a mean age of 58.8±11.1 years were enrolled and randomized to the age or placebo group in a double-blind placebo controlled trial.An anova model with treatment as the main effect was used to compare changes in cavi from baseline to follow-up between groups.The primary objective of this study was reduction in cavi over a 3-month period.In the age group, cavi was reduced on average by 0.71±1.27 vs.A mean reduction of 0.13±0.94 in the placebo group (p=0.04).On the whole, this study demonstrates that age has a positive impact on endothelial function in patients with t2dm and may play a role in the primary prevention of cardiovascular disease.

Journal: Experimental and therapeutic medicine (2020)
Publication types: Journal Article