syedzayyan/paper_mill_benchmark huggingface dataset:
Effect of CMB Carrying PTX and CRISPR/Cas9 on Endometrial Cancer Naked Mouse Model

Endometrial cancer, one of the most common gynecological cancers in women.Patients with advanced or recurrent disease have poor long-term outcomes.The current experiment explore the roles of cationic microbubbles (cmbs) carrying paclitaxel (ptx) and crispr/cas9 plasmids on the xenotransplantation model of mice with endometrial cancer.The tumor histology, tumor cell viability, cell cycle, and invasion ability were investigated.Meanwhile, the p27, p21, gsk-3, bcl-2 associated death promoter (bad), mammalian target of rapamycin (m tor), and c-erb b-2 expressions were evaluated by q rt-pcr and western blotting, respectively.Cmb-ptx-crispr/cas9 had an inhibitory action on the tumor growth, tumor cell viability, cell cycle, and invasion ability of the mouse xenograft model of endometrial cancer.The cmb-ptx-crispr/cas9 increased the gsk-3, p21, p27, and bad expression levels, while reduced the c-erb b-2 and m tor expressions.Cmbs loaded with both ptx and crispr/cas9 plasmids may be a new combination treatment with much potential.Cmb-ptx-crispr/cas9 may regulate the tumor cell viability, invasion, and metastasis of endometrial cancer naked mouse model by upregulating expressions of gsk-3, p21, p27, and bad.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Immunohistochemistry Study of OY-TES-1 Location in Fetal and Adult Human Tissues

Oy-tes-1 is reportedly involved in carcinogenesis and spermatogenesis.However, the tissue distribution of oy-tes-1 in the normal human body remains elusive.This study detected oy-tes-1 expression in human fetal and adult normal tissues by immunohistochemistry.We identified a general principle of oy-tes-1 expression.The expression of oy-tes-1 was found in neurons, smooth muscle cells, and cardiac muscle cells from both fetuses and adults.The connective tissue showed no specific staining throughout the fetal and adult samples.With oy-tes-1-positive staining of the epithelium irregular, oy-tes-1 was strongly expressed in the epithelium of the skin and bladder, as well as hepatocytes, pancreatic islets, and acinous cells during the fetal stage but was not detected in the postnatal period.In contrast to the epithelium of blood vessels, the fetal and adult central hepatic vein and glomeruli showed negative expression of the oy-tes-1 protein.Sex-dimorphism was observed in the distribution of oy-tes-1 in male and female germ cells.Collectively, our results indicate that oy-tes-1 is a member of the cancer-testis antigen and autoantigen, with tissue-specific and period-specific expression patterns, revealing potential contributions of oy-tes-1 to the diagnosis and therapeutic treatment for neoplasms and infertility.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
MiR-373 Inhibits the Epithelial-Mesenchymal Transition of Prostatic Cancer via Targeting Runt-Related Transcription Factor 2

Prostatic cancer (pca) is a prevalent form of malignancy based on its high associated levels of mortality and morbidity across the world.Micro rnas (mi rnas) are significant in the advancement of prostatic cancer.The current study is aimed at exploring the potential roles of mi r-373 in pca.In turn, the study conducted a q rt-pcr test to determine the levels of m rna.A western blot test was also executed in determining the protein level.The processes of transwell assay and wound healing were integrated in the detection of the potential for pca cells to invade and migrate.The integration of dual luciferase reporter assay is critical in determining the levels of luciferase activity among prostatic cancer cells.Then, the results showed a net decrease of mi r-373 within prostatic cancer cells and tissues.Upregulated mi r-373 reduced the invasion and migration potential of pca cells.Moreover, overexpressed mi r-373 increased the levels of e-cadherin and fsp1 as epithelial cell markers.Similarly, the overregulation of mi r-373 brought about the upregulation of mesenchymal markers (n-cadherin, snail, and vimentin).The study predicted runt-related transcription factor 2 (runx2) to be a target of mi r-373.The luciferase activity of pca cells was decreased after the cotransfection with mi r-373 mimics and runx2 3′ untranslated region (3′utr) wild type (wt).Moreover, runx2 became upregulated in pca cells and tissues.The upregulation of mi r-373 decreased the m rna and protein level of runx2.However, overexpressed runx2 abated the roles of mi r-373 in the intrusion and migration of pca cells and in regulating the expression of epithelial cell markers and mesenchymal markers.In short, mi r-373 may regulate the emt of pca cells via targeting runx2.The mi r-373/runx2 axis provides a therapeutic target for pca.

Journal: Journal of Healthcare Engineering (2021)
Publication types: Research Article;
Anti-Inflammatory and Repairing Effects of Mesoporous Silica-Loaded Metronidazole Composite Hydrogel on Human Dental Pulp Cells

In order to test an effective biopolymer scaffold in promoting the growth of human dental pulp stem cells (hdpscs), mesoporous silica @ hydrogel (msn@gel) nanocomposites are invented as a new type of biopolymer scaffold for hdpscs proliferation in this paper.The expression levels of alkaline phosphatase (alp), dentin matrix protein 1 (dmp1), and dentin sialophosphoprotein (dspp) are significantly increased in the msn@gel group so as to better repair damaged dentin.In order to inhibit the proliferation of bacteria in the dental pulp, metronidazole (mtr) is loaded into msn.The study found that msn could effectively prolong the half-life of mtr by 1.75 times, and the viability of hdpscs could be better maintained in the msn-mtr@gel group so as to better promote its proliferation to repair pulpitis.However, with the increase of the mtr concentration, its proliferation effect on hdpscs decreased gradually, and the proliferation effect is the best in 10 μmol/l.Therefore, the msn-mtr@gel scaffold is expected to become an effective method for pulpitis therapy in the future.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Expression and Related Mechanisms of miR-100 and TRIB2 in COPD Patients

Chronic obstructive pulmonary disease (copd) is one of the most common chronic respiratory diseases in the world.Copd is a general term for a class of lung diseases, including emphysema, chronic bronchitis, and refractory asthma.It is characterized by irreversible airflow obstruction and chronic tracheal inflammation.Objective.This study aimed to investigate the expression and related mechanisms of mi r-100 and trib2 in patients with copd.Methods.We collected the serum of patients admitted to our hospital and healthy volunteers undergoing physical examination at the same time, pulmonary fibroblasts were purchased for the experiments, mi r-100 was overexpressed, and trib2 expression was inhibited in cells.The mi r-100 and trib2 expression levels in serum and cells were detected by q rt-pcr and western blot, cell proliferation and apoptosis were detected by cck-8 and flow cytometry, and the relationship between mi r-100 and trib2 was explored by the dual-luciferase report.Results.The mi r-100 expression in the serum of the copd group was expressed normally, while the trib2 expression was expressed abnormally p &lt; 0.05 .The auc of serum mi r-146a and trib2 for copd diagnosis were 0.965 and 0.954, respectively.Overexpressing mi r-100 and inhibiting the trib2 expression could decrease cell proliferation and increase apoptosis rate.According to the dual-luciferase report, mi r-100 and trib2 had a targeted regulatory relationship.Rescue experiments showed that overexpressing trib2 could reverse the changes of cell proliferation and apoptosis caused by overexpression of mi r-100.Conclusion.Mi r-100 and trib2 were expressed abnormally in serum of copd patients, and mi r-100 could inhibit proliferation of pulmonary fibroblasts and promote their apoptosis.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
New Pyroptosis-Associated Gene Signature for Overall Survival Forecast among Patients Suffering from Hepatocellular Carcinoma

Due to the heterogeneity of tumors, we do not understand the given effect of pyroptosis on hepatocellular carcinoma (hcc) fully, particularly its influences on prognosis.The paper aims at exploring the prognostic value of pyroptosis-associated genes and its role in immune status in hcc.A multigene prognostic signature is set up by utilizing the least absolute shrinkage and choosing operator cox exploration.The comparison of survival between different risk groups is made with the kaplan–meier exploration and cox regression.Lasso cox regression analysis is adopted to set up a pyroptosis-associated gene signature (cep55 and mmp1).By comparing with the group at low risks, the high-risk group displayed greatly decreased os.The fatidic ability of the prognostic gene signature is confirmed by the receiver-running feature curve analysis.According to multivariate cox exploration, the risk mark is an independent predicting agent for os.A new signature established with two pyroptosis-associated genes can be adopted for prognostic forecast and influence the immune situation in hcc.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Observation on the Effect of Intelligent Machine-Assisted Surgery and Perioperative Nursing

Orthopedic surgery and care during the perioperative period are the key to the treatment of orthopedic diseases, which can quickly and effectively treat orthopedic diseases and can quickly recover during the perioperative period.Therefore, this paper focuses on the observation of the effect of intelligent machine-assisted surgery and perioperative care, combined with smart wearable devices and c-arm camera calibration; the details of the bone surgery are assisted by the machine, and then the recognition ability is accelerated by writing into the digital bone bank.Based on machine vision, cnn training and learning are designed to design a machine-assisted perioperative nursing method.This paper also designed a bone surgery test experiment and perioperative adverse event data analysis, combined with the data obtained from the experiment, designed a comparison experiment with traditional surgery and perioperative nursing.The experimental results show that the success rate of machine-assisted surgery is increased by nearly 2%–15% compared with traditional surgery; and the rehabilitation degree of machine-assisted perioperative nursing is 15.83% higher than that of traditional perioperative nursing.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Correlation Meta-Analysis of the Efficacy of Inhaled Corticosteroids in Children with Asthma Based on Smart Medical Health

Children are the reserve force for national construction, and children’s health is now being paid more and more attention.In today’s childhood asthma treatment, many are based on the treatment of common cough, and there is no strict division with asthma.Importantly, the efficacy of inhaled corticosteroids in the treatment of childhood asthma has been extensively studied in the medical community.However, there is no clear explanation for its specific efficacy and role.Research on childhood asthma has always been a key topic in medicine.This article aims to conduct a meta-analysis of the correlation between inhaled corticosteroids and asthma in children.This article proposes a more scientific literature screening strategy, which can achieve a higher accuracy rate for meta-analysis.It combines the intelligent medical health system in the analysis of children’s asthma to assist in the analysis of curative effects.Such analysis also provides reference significance for related research.The experimental results indicate that inhaled corticosteroids have a good effect on childhood asthma.As the concentration of inhaled glucocorticoid increases, the effect will be better.Specifically, if the concentration is increased by 10%, the effect will be about 15% better, but there are corresponding side effects.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Meta-Analysis;
The Effects of Febuxostat on Urine NGAL and Urine KIM-1 in Patients with Hyperuricemia

Retrospective analysis of the effects of febuxostat on urine neutrophil gelatinase-associated lipocalin (ngal) and kidney injury molecule-1 (kim-1) in patients with hyperuricemia was performed.From january 2018 to june 2018, there were 45 patients with asymptomatic hyperuricemia in the outpatient or inpatient of changzhou second people’s hospital, which were divided into the febuxostat group (25 cases) and the control group (20 cases).We collected the patients’ baseline indicators and testing indicators after three months of treatment, including blood urea nitrogen, blood creatinine, blood uric acid, urine microalbumin, urine ngal, urine kim-1, and other indicators.The subjects in both groups were given lifestyle intervention, instructed to drink more water, and given a low-purine diet.The patients in the febuxostat group took febuxostat 40 mg/d or 80 mg/d.We used spss 25.0 statistical software for statistical analysis.Baseline indexes between the febuxostat group and the control group and indexes after treatment between two groups were both performed by independent sample t-test, and paired t-test was used for self-comparison between the groups before and after treatment.There was no significant difference in age, sex, body mass index (bmi), urea nitrogen, creatinine, uric acid, urine microalbumin/creatinine, urine ngal/creatinine, and urine kim-1/creatinine between the two groups before treatment ( p &gt; 0.05 ).Compared with before treatment, after 3 months of intervention, the levels of serum uric acid, urine microalbumin/creatinine, urine ngal/creatinine, and urine kim-1/creatinine were significantly decreased in the febuxostat group ( p &lt; 0.05 ), while the changes of blood urea nitrogen, serum creatinine, and epidermal growth factor receptor (e gfr) were not statistically significant ( p &gt; 0.05 ).After 3 months of intervention, the control group had no significant changes in blood urea nitrogen, creatinine, e gfr, uric acid, microalbumin/creatinine, urine ngal/creatinine, and urine kim-1/creatinine ( p &gt; 0.05 ).After 3 months of intervention, compared with the control group, the serum uric acid, microalbumin/creatinine, urine ngal/creatinine, and urine kim-1/creatinine were significantly decreased in the febuxostat group ( p &lt; 0.05 ), but there was no significant difference in blood urea nitrogen, creatinine, and e gfr ( p &gt; 0.05 ).Febuxostat can reduce urine ngal/creatinine and urine kim-1/creatinine levels in patients with hyperuricemia and has the protective effects on renal tubular injury caused by hyperuricemia, which can provide evidences for the early prevention and treatment of asymptomatic hyperuricemia.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
hMSCs Migrate under the Chemotaxis of CXCL-13 and Enhance Islet B Cell Activity through p-AKT Signaling Pathway in High-Glucose Environment

As a common clinical chronic disease, the incidence of diabetes is increasing year by year.According to the latest statistics from the international diabetes federation, as of 2019, the global prevalence of diabetes has reached 8.3%.This study aims to investigate the effect of cxcl-13 on the migration ability of human mesenchymal stem cells (h mscs) and to clarify the specific molecular mechanism of the protective effect of h mscs on islet b cells.The h mscs were cultured in high-glucose environment, and the effect of cxcl-13 on the migration ability of h mscs was determined by transwell experiment.After coculture of h mscs and islet b cells, the activity of cells was detected by cck8 assay, the expression of ki-67 in cells was detected by rt-pcr, and the expression of p53 was detected by western blot to investigate the effect of h mscs on the proliferation and apoptosis of islet b cells.The effect of h mscs on the function of islet b cells was determined by glucose stimulated insulin secretion experiment.Transwell experiment results showed that cxcl-13 could promote the migration of h mscs to islet b cells in high-glucose environment.The results of cck-8 showed that the cell activity in the coculture group was significantly higher than that of the other groups, and rt-pcr showed that the expression of ki-67 was significantly increased in the coculture group of h mscs and islet b cells.The results of western blot showed that the expression of p53 was significantly decreased in the coculture group, and the glucose stimulated insulin secretion test showed that insulin secretion was significantly increased.It was found that after the inhibition of atk, cell activity was significantly reduced, and apoptosis was significantly increased.Meanwhile, the expression of ki-67 was inhibited, the expression of p-53 was significantly increased, and insulin secretion was significantly reduced.To sum up, in a high-glucose environment, cxcl-13 effectively promoted the migration of h mscs, and h mscs protected the activity and function of islet b cells through akt signaling pathway.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Mechanism of Pilose Antler in Treatment of Osteoporosis Based on Network Pharmacology

The purpose of this study was to demonstrate the pharmacodynamic material basis and molecular mechanism of pilose antler (pa) in the prevention and treatment of osteoporosis (op) by the method of network pharmacology.Methods.First, the active components of pa were screened by batman-tcm database, and the component targets were obtained from the swiss target prediction online tool.Moreover, the relevant target genes of op were obtained by searching the dis ge net database.Second, the venn diagram was drawn to obtain the pa-op common targets, and the protein-protein interaction (ppi) network and drug-component-target (d-c-t) network were constructed by cytoscape software.Finally, the go functional annotation and kegg pathway enrichment analysis of common targets were performed using the metascape online tool.Results.82 common targets were identified by generating a venn diagram.The ppi network of 82 common targets indicated that the top 5 nodal targets, including pik3ca, mapk1, esr1, akt1, and src, were strongly associated with other proteins.The d-c-t network suggested that the active components with high degree of connectivity include prostaglandin e1, 17-beta-estradiol, alpha-estradiol, and estrone.Furthermore, the go enrichment analysis revealed that the biological process categories were dominated by response to peptide, cellular response to lipid, regulation of mapk cascade, and so on.Additionally, the kegg pathway analysis indicated the estrogen signaling pathway, osteoclast differentiation, and hif-1 signaling pathway might have critical effects on the development of op.Conclusion.The study shows that pa has the characteristics of multi-component, multi-target, and multi-pathway in treating osteoporosis.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Panax Notoginseng Saponin Rg1 Can Effectively Improve the Cognitive Function of 5 × FAD Mice

In order to investigate the effect of notoginsenoside rg1 on cognitive function in 5 ∗ fad mice and the mechanism of cx43 in improving cognitive function in mice, the methods 5 ∗ fad mice are selected as experimental animals and normal mice as healthy control.They were divided into three groups: healthy control group (n = 10), disease group (n = 10), and treatment group (n = 10, panax notoginsenoside rg 1150 mg/kg/d) for 2 months.Two months later, three groups of mice were subjected to classical water maze and y-maze to test the cognitive ability of mice, and the correct response times and total response time were recorded.At the end of the cognitive function test, the mice were executed, and the brain tissues were taken.The expression of cx43 protein and the changes of glial cells and neurons in the brain of the mice were analyzed at the cellular level.After treatment with panax notoginseng saponin rg 1150 mg/kg/d, it was found that the escape latency of mice in the treatment group was significantly lower than that in the disease group from the third day of training ( p &lt; 0.05 ); the time spent on the platform quadrant and the number of times crossing the platform in the treated mice were significantly increased, and the difference was statistically significant ( p &lt; 0.05 ); the expression of cx43 protein in the brain of mice after treatment was significantly higher than that of mice in the disease group ( p &lt; 0.05 ).Conclusion.Panax notoginseng saponin rg1 can effectively improve the cognitive function of 5 ∗ fad mice by increasing the secretion of cx43 protein, thus increasing the reactivity of glial cells and neurons.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;
Cytotoxic Action of Palladium-Based Compound on Prostate Stem Cells, Primary Prostate Epithelial Cells, Prostate Epithelial Cells, and Prostate Cell Lines

Prostate cancer is one of the most common types of cancer found to occur in males and is ranked as the second-highest cause of cancer-associated deaths among male patients.In this study, we have shown the influence of a new palladium-based anticancer agent in contrast to the six distinct prostate cancer lines and the primary cultures.Methods.In this study, we have used six distinct prostate cell lines, that is, pnt2-c2, lnca p, bph-1, pc-3, pnt1a, and p4e6.The mtp and atp assay were performed to evaluate the growth of the cell and the flow cytometry to investigate the status of the cell cycle.The antigrowth effect of the palladium complex was evaluated against different cell lines at three time zones 24 h, 48 h, and 72 h.[pd cl(terpy)] (capsule)-2h2o is synthesized by direct encapsulation of equimolar amounts of capsule ions into [pd (terpy) cl] cl-2h2o.Results.A comparative analysis was done on 25 m m etoposide and 12 m m cisplatin, cytotoxic agents.The lowest ic50 value at 72 hours was 0.128 m m for bph-1 cell lines with 0.139 m m, whereas pnt2-c2 cells were found to be most resistant with ic50 values of 0.829 m m.The antigrowth effect of palladium complex on cell lines was measured using the mts assay at 24, 48, and 72 hours.Bph-1, pnt2-c2, and pnt1a either possess normal tissues or have benign prostatic hyperplasia tissues whereas p4e6, pc-3, and lnca p cell lines possess malignant origin.The pd complex exhibited significant cytotoxic action in stem cells when compared against etoposide.An antigrowth effect was reported for pd complex at lower concentration, but it was more cytotoxic than etoposide with significant cytotoxicity p = 0.001 .Conclusion.The palladium complex experienced a substantial antigrowth influence over most of the prostate tumor cell lines and the primary cultures, eventually, leading to the implementation of this pd complex in the treating procedure of metastatic prostate cancer, which is tremendously resistant to the traditional treatment.

Journal: Journal of Healthcare Engineering (2022)
Publication types: Research Article;