syedzayyan/paper_mill_benchmark huggingface dataset:
Increased B Cell-Activating Factor Expression Is Associated with Postoperative Recurrence of Chronic Rhinosinusitis with Nasal Polyps.

Chronic rhinosinusitis with nasal polyps (crsw np) is a common upper airway inflammatory disease with a high postoperative recurrence rate.This study is aimed at exploring the expression of b cell-activating factor (baff) in crsw np and its association with postoperative recurrence.Methods a total of 80 crsw np patients, including 40 primary crsw np patients and 40 recurrent crsw np patients, 40 chronic rhinosinusitis without nasal polyps (crss np) patients, and 40 healthy controls (hc) were enrolled in this study, and the serum and tissue samples were collected.The circulating and tissue baff expressions were detected by enzyme-linked immunosorbent assay reverse transcription-polymerase chain reaction and immunohistochemistry.Their clinical values for predicting postoperative recurrence of crsw np were evaluated.Results we determined serum levels of baff were remarkably increased in the crsw np group than the crss np and hc groups (p< 0.05), and higher concentrations of baff were associated with peripheral eosinophil percentage (r= 0.614,p< 0.001).The serum baff concentrations were significantly higher in the recurrent crsw np group in comparison with the primary group (p< 0.05).Multivariate analysis and receiver operating characteristic (roc) curve presented that serum baff levels were associated with the postoperative recurrence in crsw np patients (p< 0.05).Moreover, tissue baff levels were significantly increased in the crsw np group than the hc group, especially in the recurrent crsw np group (p< 0.05), and enhanced baff rna expressions were correlated with serum baff levels (r= 0.703,p< 0.001).Conclusion our results elucidated that the baff expression was enhanced in crsw np patients and associated with postoperative recurrence.Baff could be a serologic biomarker for predicting postoperative recurrence in crsw np patients.

Journal: Mediators of inflammation (2022)
Publication types: Journal Article
Melatonin improves meiosis maturation against diazinon exposure in mouse oocytes.

S organophosphorus pesticide diazinon (dzn) has adverse effects on animals and humans by direct contact or the spread of food chain.The antioxidant melatonin has protective effects on female reproduction.This study aimed to explore the effects of dzn on meiosis maturation in mouse cumulus oocyte complexes (cocs) and the effects of melatonin.Main methods different concentrations of dzn and melatonin were added during the in vitro maturation of cocs.Then we detected the extrusion rate of the first polar body, the number of sperms binding to oocyte, mitochondrial membrane potential, reactive oxygen species (ros), early apoptosis.Subsequently, the expression of juno, cx37, cx43 and erk1/2 were detected by immunofluorescence staining and western blotting.Key findings dzn exposure results in the failure of nuclear and cytoplasmic maturation of oocyte meiosis.Destruction of repositioning and function of mitochondria increases the levels of ros and early apoptosis.The dzn-exposed oocytes express less juno resulting to bind less sperms than normal.The loss of gap junctions and failure to activate erk1/2 also contribute to the failure of cytoplasmic maturation.All these ultimately lead to the poor oocyte quality and low fertility.Appropriate melatonin can effectively restore all these defects.Significance under dzn exposure, melatonin can significantly improve the quality of oocytes, and melatonin promotes oocyte maturation by protecting gap junction and restoring erk1/2 pathway, which is a new breakthrough for improving female fertility.

Journal: Life sciences (2022)
Publication types: Journal Article
Tumor-targeting pH/redox dual-responsive nanosystem epigenetically reverses cancer drug resistance by co-delivering doxorubicin and GCN5 siRNA.

Multidrug resistance (mdr) is a major cause accounting for chemotherapy failure and recurrence of malignant tumors.A prominent mechanism underlying mdr is overexpression of p-glycoprotein (p-gp, a drug efflux pump).Promoting drug delivery efficacy by targeting tumor and concurrently suppressing drug efflux through down-regulating p-gp emerges as an effective strategy to enhance intracellular drug accumulation for combating mdr tumor.General control non-repressed 5 (gcn5), a histone acetyltransferase acting as an epigenetic regulator of multidrug resistance protein 1 (mdr1), positively regulates p-gp levels in drug-resistant cancer cells.Herein, a hyaluronic acid-coated, p h/redox dual-responsive nanosystem (hpmsns) is fabricated for co-delivering doxorubicin (dox) and gcn5 si rna (si gcn5).This nanosystem can effectively encapsulate dox and si rna preventing premature leakage and releasing these therapeutics intracellularly via its p h/redox dual responsiveness.Through cd44-mediated targeting, dox/si gcn5@hpmsns increases drug internalization in cd44-overexpressing cancer cells, and markedly promotes dox retention by down-regulating p-gp expression in drug-resistant cancers through silencing gcn5.Of note, in an mdr breast tumor model, dox and si gcn5 co-delivered hpmsns inhibits mdr tumor growth by 77%, abolishes p-gp-mediated drug resistance, and eliminates dox's systemic toxicity.Thus, the tumor-targeting, stimuli-responsive nanosystem is an effective carrier for co-delivering anticancer drug and si rna for combating cancer drug resistance.Statement of significance: we designed a tumor-targeting, p h/redox dual-responsive nanosystem (hpmsns) for chemo-drug and si rna co-delivery.This nanosystem efficiently co-delivered dox and si gcn5 into drug-resistant cancer cells and significantly inhibited the tumor growth through: (1) ha shell enhanced the cellular internalization of loaded dox and si gcn5 via cd44-mediated targeting; (2) the p h/redox dual-responsive nanosystem released the cargos in response to the intracellular environment; (3) the released si gcn5 downregulated p-gp epigenetically.In an mdr breast tumor model (mcf7/adr), dox and si gcn5 loaded hpmsns markedly inhibited tumor growth, almost completely abolished p-gp expression, and minimized systemic toxicity of dox.

Journal: Acta biomaterialia (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Flocculation of Chlorella vulgaris-induced algal blooms: critical conditions and mechanisms.

Algal blooms have posed great threats to livestocks and human health.Although flocculation is effective, its efficiency may hinder the direct application for algal blooms.In this study, critical (optimal) conditions and mechanisms for al cl3, fe cl3, poly-aluminum chloride (pac), chitosan, and polydimethyldiallylammonium chloride (pdadmac)-induced flocculation of chlorella vulgaris (c.Vulgaris) were studied.Results identified the critical conditions which can cause flocculation efficiencies over 90% in 45 min for the five flocculants.Specifically, 4~10-mg/l doses of pdadmac were proved to be appropriate for the treatment of c.Vulgaris-induced algal blooms at p h 6.0~12.0.To probe the underlying mechanisms, functional groups involved in flocculation, zeta potential, and species distribution were analyzed during flocculation.Ft-ir results indicated that n-h stretching in amine and c-h deformation in aliphatics were involved in algal flocculation with fe cl3, and c-h deformation played an important role with pdadmac, pac, and chitosan.For al cl3, zeta potential and species distribution results suggested that charge neutralization and adsorption bridging were responsible for algal flocculation at p h 6~8.However, adsorption bridging and sweeping effects were the main mechanisms at p h >3 for fe cl3.The flocculation mechanisms for the rest of the three polymers were charge neutralization, adsorption bridging, and sweeping.Meanwhile, all the flocculation processes followed second-order kinetics.Strong linkages were found between the rate constant, fractal dimension, and flocculation efficiency (p < 0.05).The results of critical flocculation conditions and mechanisms indicated that pdadmac was an excellent flocculant for c.Vulgaris removing and recycling, especially in water bloom treatment.

Journal: Environmental science and pollution research international (2022)
Publication types: Journal Article
Metal-Free Synthesis of N-Alkyl-2,5-Unsubstituted/Monosubstituted Fulleropyrrolidines: Reaction of [60]Fullerene with Paraformaldehyde and Amines.

A series of scarce n-alkyl-2,5-unsubstituted/monosubstituted fulleropyrrolidines were synthesized in moderate to excellent yields by the simple one-step thermal reaction of [60]fullerene with primary/secondary amines in the presence of paraformaldehyde without the addition of valuable metal salts.Intriguingly, the reaction with primary amines unexpectedly afforded n-alkyl-2,5-unsubstituted fulleropyrrolidines instead of the anticipated 2,5-monosubstituted fulleropyrrolidines.A plausible reaction pathway is proposed to elucidate the above-mentioned reaction process based on the experimental results.

Journal: The Journal of organic chemistry (2019)
Publication types: Journal Article
Chronic maternal hypercortisolemia models stress-induced adverse birth outcome and altered cardiac function in newborn lambs.

Maternal stress in pregnancy is thought to be a contributing factor in adverse pregnancy outcomes, including stillbirth and prematurity.Previous studies in our laboratory have shown that chronic elevation in maternal cortisol concentration in ewes (by maternal infusion of 1 mg/kg/day) during the late gestation increased the incidence of stillbirth and altered fetal heart rate and blood pressure at birth.We designed the current study to test the effect of chronically elevated maternal cortisol on fetal cardiac adaption from in utero life to ex utero life.The combined risk of stillbirth or prematurity was significantly greater in the pregnancies with maternal hypercortisolemia; in this cohort, 40% of the lambs of cortisol-infused ewes died in utero or at birth compared with 25% of lambs of control ewes, and 24% of lambs of cortisol-infused ewes were born preterm, whereas no lamb was born preterm in the control group.Compared with control lambs, the lambs of cortisol-infused ewes born at full term exhibited a significant increase in mean aortic pressure just before birth and a significant decrease in mean aortic pressure that was evident during the first 9 h after birth.The qt interval was decreased before birth and increased immediately after birth in the newborns of cortisol-treated ewes compared with control lambs.These findings suggest that excess in utero corticosteroid exposure adversely affects fetal cardiac adaptation to extrauterine life and that chronic maternal stress or hypersecretion of corticosteroids may contribute to adverse obstetric outcomes.

Journal: American journal of physiology. Regulatory, integrative and comparative physiology (2022)
Publication types: Journal Article;Research Support, N.I.H., Extramural
A New Fossil Species ofNothotsugafrom the Mula Basin, Litang County, Sichuan Province and Its Paleoclimate and Paleoecology Significance.

In this paper, we describe a new fossil species, nothotsuga mulaensis z.Li & j.l.Dong sp.Nov.The discovery of the fossil species was based on well-preserved fossil seed cones that were found in the mula basin in xiamula village, litang county, sichuan province, southwestern china.The shapes of these fossils were characterized by ovate seed cones, rhombic or suborbicular scales with auriculate bases, and the bracts were ligulate-spathulate in shape.This finding suggests that nothotsuga once had a wide distribution range in china and that it also inhabited the eastern tibetan plateau (tp).Nothotsuga mulaensis was distributed in an intermountain lake basin, at altitudes from 2000 to 2300 m, in a warm and humid environment.This finding also suggests that the eastern tp may have provided good habitat for nothotsuga during the miocene.In addition, we propose that the uplift, accompanied by the severe cooling and aridification that occurred after the miocene, caused the disappearance of this species of nothotsuga in the eastern tp.

Journal: Biology (2022)
Publication types: Journal Article
Spiroligustolides A and B: Two pairs of enantiomeric spiro-orthoester-containing phthalide dimers as Cav3.1 calcium channel inhibitors from Ligusticum Chuanxiong Hort.

Two pairs of unprecedented enantiomeric phthalide dimers, spiroligustolides a (1a/1b) and b (2a/2b), featuring a unique spiroorthoster linkage between two monomeric units to form a 5/6/5/6/6-fused ring system, were isolated from the roots of ligusticum chuanxiong.The structures and relative configurations of 1 and 2 were determined by hr-esi-ms, ir, and nmr spectroscopic data, coupled with single-crystal x-ray diffraction analysis, and the absolute configurations of 1a, 1b, 2a, and 2b were established by comparing the experimental and calculated electronic circular dichroism (ecd) data.Plausible biosynthetic pathway for 1 and 2 was proposed.Moreover, compounds 1, 1b, and 2b showed remarkable inhibitory activities on cav3.1 calcium channel with ic50values of 8.34, 7.08, and 8.60 μm, respectively.

Journal: Bioorganic chemistry (2022)
Publication types: Journal Article;Research Support, Non-U.S. Gov't
Profiles of Childhood Victimization as Predictors of Sexual Aggression and Intimate Partner Violence Perpetration.

The study utilized a latent profile analysis to evaluate distinct combinations of men's childhood victimization experiences and their communal predictive ability for sexual aggression (sa) and intimate partner violence (ipv) perpetration.Men living in the united states (n= 399) completed assessments of emotional, physical, and sexual childhood victimization, as well as sa and ipv toward women.The results indicated that members of profiles characterized by moderate to high frequencies of childhood victimization-especially sexual victimization-were at increased risk of sa and ipv perpetration.Contrary to expectations, adult- versus peer-perpetrated victimization did not appear to substantially alter risk of perpetration.

Journal: Violence against women (2022)
Publication types: Journal Article
A Sensitive Approach to Managing Hypersensitivity Pneumonitis.

Hypersensitivity pneumonitis (hp), also known as extrinsic allergic alveolitis, is an immunologically mediated disorder that typically presents as a case of interstitial lung disease (ild) in response to any identified or unidentified antigen.We present a case of a 46-year-old female with hp, who presented with fever and shortness of breath.Although negative by real-time reverse transcription-polymerase chain reaction (rt-pcr), her condition was initially diagnosed as covid-19 clinically as a result of anchoring bias due to similar symptoms and radiologic features presenting in the pandemic.A detailed further probing into history revealed the diagnosis of hp due to cat hair, and hence, was managed accordingly.

Journal: Cureus (2022)
Publication types: Case Reports;Journal Article
Analysis of Multi-Part Phenotypic Changes in Skin to Characterize the Trajectory of Skin Aging in Chinese Women.

As the human body's largest organ exposed to the external environment, the skin suffers from internal and external aging factors, leading to wrinkles, loss of elasticity, sagging, and rough appearance.However, little is known of the characteristics of skin aging of different body parts in chinese women.Here, we study the signs of extrinsic skin aging in different body parts to identify the knowledge map of manifestations of aging in chinese women.Patients and methods wrinkle and texture phenotypes and collagen samples from the face, neck, hands, and arms of 326 chinese women were collected.The correlations between phenotypes and ages and the differences in phenotypes by age were evaluated.Results the wrinkle and texture phenotypes around the eyes and mouth and of the hands were strongly correlated with age.Ages 32 and 58 showed the largest number of differentially changed aging phenotypes.The number of aging phenotypes increased sharply between the ages of 24 and 30, suggesting that the skin was undergoing rapid aging.Eye aging was the most rapidly changing phenotype between 19 and 30 years old.Wrinkles at the corner of the eyes showed a significant difference in the older group, suggesting an early onset and long-term effects.Conclusion this is the first study to be performed on the characteristics of skin aging among chinese women that takes account of multiple areas of the body.It was found that 24 years old was the time point at which the skin begins to age in chinese women.This provides important clues for aging-related research and personalized skin care.

Journal: Clinical, cosmetic and investigational dermatology (2022)
Publication types: Journal Article
The non-equilibrium basis of Turing Instability and localised biological work.

Turing's theory for biological pattern formation is based on the instability of the homogeneous state, which occurs if certain key criteria are met.The problem of how chemical energy is converted to localised biological work requires one to understand not only the basis of localised power generation, but also the age-old puzzle of how organisms decrease their entropy; these problems can only be solved by the identification of the turing instability.At the heart of this is how natural selection, not chemistry, has fashioned the large non-equilibrium overall affinity (δg is a large negative quantity) for the oxidation of the fuel molecules.Natural selection has also resulted in the homeostasis at non-equilibrium values of the hydrolysis of molecules like atp, gtp, which are the energy links between the overall oxidation of the fuel and biological work.The conditions for such homeostasis are central requirements for the turing instability and are the essence of being alive.The turing-child (tc) patterns are the spontaneous primary spatial cause not only of localised biological work in multicellular systems (especially those in patterning and development) but also of intracellular patterns including the mitotic spindle and the contractile ring.The turing picture comprises the nonuniform distribution of the concentrations of the turing morphogens, c amp and atp, and the child picture is the resulting nonuniform distribution of the metabolic rate and of power.The tc pattern is shaped as the dominant eigenfunction in the combination of eigenfunctions which provides the spatial pattern of the turing morphogens.The tc patterns and the bifurcation parameter manifest quantisation and symmetry as in music and in applications of quantum mechanics.The notion of correlation diagrams is also introduced.

Journal: Progress in biophysics and molecular biology (2017)
Publication types: Journal Article;Review
Optimization of citric acid production byAspergillus nigerusing two downgraded Algerian date varieties.

In the present work, the ghars and the mech degla downgraded date varieties were used in a fermentation medium in order to produce citric acid by the aspergillus niger.The biochemical characteristics of the dates were investigated, along with the chemical and physical characteristics of the solutions of both samples.The analyzed parameters included the moisture and sugar content, the ash residual, the p h values, and the electrical conductivity.The effect of the following fermentation parameters was studied: initial p h, temperature, incubation period, and methanol.For the ghars and mech degla date varieties respectively, the ash residual measured at 1.90% and 2.47%.For each date variety, the moisture and total sugars were measured at 11.59% and 85%, for the ghars, and 12.82% and 80.47% for the mech degla.Citric acid production using either of the two varieties of dates showed a high yield in a short time.The obtained results showed that the highest production of citric acid by both medium of dates was achieved at the initial p h value of 3.0, temperature 30 °c, and an incubation period of 8 days.Also, the maximum amount of citric acid was produced when both mediums contained 4% of methanol.Both varieties of dates showed a good yield for the citric acid and can be used as a culture medium since they are economic and ensure good growth for the aspergillus niger.

Journal: Saudi journal of biological sciences (2021)
Publication types: Journal Article
Prevalence and factors associated with depression in people living with HIV/AIDS in a Brazilian metropolitan region.

The frequent association between mental disorders and hiv infection may be related to several risk factors.The present study aims to determine the prevalence of depression and its associated factors in people living with hiv/aids (plwha) who attended four outpatient clinics in fortaleza, brazil, between september 2014 and april 2015.In addition to the mini international neuropsychiatric interview questionnaire, which was used to identify the prevalence of depressive disorder, the researchers applied a sociodemographic questionnaire, and the hamilton depression scale to classify the severity of depression.In all, 257 participants were included in the analyses.The overall lifetime prevalence of depression was identified to be 29.2% (19.5% in men and 44.9% in women).The prevalence of a current depressive episode was 18.7%.Mild, moderate, and severe depression was identified in 2.1%, 12.5%, and 85.4%, respectively.The factors related to depression were: gender, marital status, income, educational level, current employment status, and family history of depression.This study highlights the importance of lifetime depression as a disorder of high prevalence in plwha, and associated with risk factors that are difficult or impossible to be modified.Early diagnosis and treatment of depression may improve quality of life in plwha.

Journal: AIDS care (2022)
Publication types: Journal Article
Dynamics of breast milk antibody titer in the six months following SARS-CoV-2 infection.

While a growing body of literature describes antibody dynamics in serum, little is known about breast milk antibody titers in the months following sars-co v-2 infection.Objectives we evaluated the dynamics of the humoral immune response to sars-co v-2 in two women who were breastfeeding when infected.We assessed paired breast milk and serum samples for six months post-infection for antibodies specific to the sars-co v-2 receptor binding domain (rbd) of the spike protein.Results starting at 10 days after symptom onset, ig a antibody levels were persistent over a 6-month time period in human milk.For both mothers, no detectable ig a was found in the samples collected pre-symptom onset.Rbd-specific ig g and ig m antibodies in tandem serum collected from the two donors demonstrated stable ig g levels over the six-month time period post-symptom onset.Conclusions we found that breastfeeding mothers produced a durable ig a response for up to six months following covid-19 infection, suggesting an important role for breast milk in protection of infants.

Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (2021)
Publication types: Journal Article;Research Support, Non-U.S. Gov't