2 D IA 1 In order to test this hypothesis , 1 a double-stranded oligonucleotide probe that corresponds to bp +10 to +60 of the CCR3 gene was prepared , 3 0 NN referred to as E1-FL ( exon 1- full length , Figure 2A ) . 3 D A 1 1 This is the exact sequence 3 D N that was deleted in the CCR3 ( -exon1 ) .pGL3 plasmid 3 D N that demonstrated decreased activity 3 D N 1 compared to the full length 1.6 kb construct [ 27 ] .