sujigwak/NVAR huggingface dataset:
UniProt ID: O07678
Variant: G -> E at position 50-50
Variant annotation: in strain: NCTC 11638
Protein function: Methylates ribosomal protein L11.
Reference sequence:
MLKPMYYEFFFIFPKERELFESFLLDATHLALEESSLENLKAFDDKETIGFISQSNWHYFATHDPLKKDLKENLKEKPPHLKNFVILRSQKDLNNSLIPALEAFCLNLKQNLQSEFDFFYLSRNLASKDWLEAYKQAILPVQCTKFYIHPSWHQKPSHVVTNDCIMIDPALAFGSGHHESTSMCLELLSDIDLKRKNALDVGCGSGILSIALKKQGVSALVACDTDSLAVEETLKNFSLNQIPLLVQDKVIYGSTQKIEGRFDVIVANLVADVIKSLYSEFVRLCNHTLILSGILETHLNSVLQIYYNGFEVLEQRQRNEWVALKLLKKQPIN
UniProt ID: O07678
Variant: A -> T at position 61-61
Variant annotation: in strain: NCTC 11638
Protein function: Methylates ribosomal protein L11.
Reference sequence:
MLKPMYYEFFFIFPKERELFESFLLDATHLALEESSLENLKAFDDKETIGFISQSNWHYFATHDPLKKDLKENLKEKPPHLKNFVILRSQKDLNNSLIPALEAFCLNLKQNLQSEFDFFYLSRNLASKDWLEAYKQAILPVQCTKFYIHPSWHQKPSHVVTNDCIMIDPALAFGSGHHESTSMCLELLSDIDLKRKNALDVGCGSGILSIALKKQGVSALVACDTDSLAVEETLKNFSLNQIPLLVQDKVIYGSTQKIEGRFDVIVANLVADVIKSLYSEFVRLCNHTLILSGILETHLNSVLQIYYNGFEVLEQRQRNEWVALKLLKKQPIN
UniProt ID: O07678
Variant: P -> S at position 65-65
Variant annotation: in strain: NCTC 11638
Protein function: Methylates ribosomal protein L11.
Reference sequence:
MLKPMYYEFFFIFPKERELFESFLLDATHLALEESSLENLKAFDDKETIGFISQSNWHYFATHDPLKKDLKENLKEKPPHLKNFVILRSQKDLNNSLIPALEAFCLNLKQNLQSEFDFFYLSRNLASKDWLEAYKQAILPVQCTKFYIHPSWHQKPSHVVTNDCIMIDPALAFGSGHHESTSMCLELLSDIDLKRKNALDVGCGSGILSIALKKQGVSALVACDTDSLAVEETLKNFSLNQIPLLVQDKVIYGSTQKIEGRFDVIVANLVADVIKSLYSEFVRLCNHTLILSGILETHLNSVLQIYYNGFEVLEQRQRNEWVALKLLKKQPIN
UniProt ID: O07678
Variant: P -> S at position 78-78
Variant annotation: in strain: NCTC 11638
Protein function: Methylates ribosomal protein L11.
Reference sequence:
MLKPMYYEFFFIFPKERELFESFLLDATHLALEESSLENLKAFDDKETIGFISQSNWHYFATHDPLKKDLKENLKEKPPHLKNFVILRSQKDLNNSLIPALEAFCLNLKQNLQSEFDFFYLSRNLASKDWLEAYKQAILPVQCTKFYIHPSWHQKPSHVVTNDCIMIDPALAFGSGHHESTSMCLELLSDIDLKRKNALDVGCGSGILSIALKKQGVSALVACDTDSLAVEETLKNFSLNQIPLLVQDKVIYGSTQKIEGRFDVIVANLVADVIKSLYSEFVRLCNHTLILSGILETHLNSVLQIYYNGFEVLEQRQRNEWVALKLLKKQPIN
UniProt ID: O07678
Variant: QKD -> EEN at position 90-92
Variant annotation: in strain: NCTC 11638
Protein function: Methylates ribosomal protein L11.
Reference sequence:
MLKPMYYEFFFIFPKERELFESFLLDATHLALEESSLENLKAFDDKETIGFISQSNWHYFATHDPLKKDLKENLKEKPPHLKNFVILRSQKDLNNSLIPALEAFCLNLKQNLQSEFDFFYLSRNLASKDWLEAYKQAILPVQCTKFYIHPSWHQKPSHVVTNDCIMIDPALAFGSGHHESTSMCLELLSDIDLKRKNALDVGCGSGILSIALKKQGVSALVACDTDSLAVEETLKNFSLNQIPLLVQDKVIYGSTQKIEGRFDVIVANLVADVIKSLYSEFVRLCNHTLILSGILETHLNSVLQIYYNGFEVLEQRQRNEWVALKLLKKQPIN
UniProt ID: O07678
Variant: P -> L at position 99-99
Variant annotation: in strain: NCTC 11638
Protein function: Methylates ribosomal protein L11.
Reference sequence:
MLKPMYYEFFFIFPKERELFESFLLDATHLALEESSLENLKAFDDKETIGFISQSNWHYFATHDPLKKDLKENLKEKPPHLKNFVILRSQKDLNNSLIPALEAFCLNLKQNLQSEFDFFYLSRNLASKDWLEAYKQAILPVQCTKFYIHPSWHQKPSHVVTNDCIMIDPALAFGSGHHESTSMCLELLSDIDLKRKNALDVGCGSGILSIALKKQGVSALVACDTDSLAVEETLKNFSLNQIPLLVQDKVIYGSTQKIEGRFDVIVANLVADVIKSLYSEFVRLCNHTLILSGILETHLNSVLQIYYNGFEVLEQRQRNEWVALKLLKKQPIN
UniProt ID: O07678
Variant: N -> S at position 107-107
Variant annotation: in strain: NCTC 11638
Protein function: Methylates ribosomal protein L11.
Reference sequence:
MLKPMYYEFFFIFPKERELFESFLLDATHLALEESSLENLKAFDDKETIGFISQSNWHYFATHDPLKKDLKENLKEKPPHLKNFVILRSQKDLNNSLIPALEAFCLNLKQNLQSEFDFFYLSRNLASKDWLEAYKQAILPVQCTKFYIHPSWHQKPSHVVTNDCIMIDPALAFGSGHHESTSMCLELLSDIDLKRKNALDVGCGSGILSIALKKQGVSALVACDTDSLAVEETLKNFSLNQIPLLVQDKVIYGSTQKIEGRFDVIVANLVADVIKSLYSEFVRLCNHTLILSGILETHLNSVLQIYYNGFEVLEQRQRNEWVALKLLKKQPIN
UniProt ID: O07678
Variant: K -> Q at position 109-109
Variant annotation: in strain: NCTC 11638
Protein function: Methylates ribosomal protein L11.
Reference sequence:
MLKPMYYEFFFIFPKERELFESFLLDATHLALEESSLENLKAFDDKETIGFISQSNWHYFATHDPLKKDLKENLKEKPPHLKNFVILRSQKDLNNSLIPALEAFCLNLKQNLQSEFDFFYLSRNLASKDWLEAYKQAILPVQCTKFYIHPSWHQKPSHVVTNDCIMIDPALAFGSGHHESTSMCLELLSDIDLKRKNALDVGCGSGILSIALKKQGVSALVACDTDSLAVEETLKNFSLNQIPLLVQDKVIYGSTQKIEGRFDVIVANLVADVIKSLYSEFVRLCNHTLILSGILETHLNSVLQIYYNGFEVLEQRQRNEWVALKLLKKQPIN
UniProt ID: O07678
Variant: T -> A at position 144-144
Variant annotation: in strain: NCTC 11638
Protein function: Methylates ribosomal protein L11.
Reference sequence:
MLKPMYYEFFFIFPKERELFESFLLDATHLALEESSLENLKAFDDKETIGFISQSNWHYFATHDPLKKDLKENLKEKPPHLKNFVILRSQKDLNNSLIPALEAFCLNLKQNLQSEFDFFYLSRNLASKDWLEAYKQAILPVQCTKFYIHPSWHQKPSHVVTNDCIMIDPALAFGSGHHESTSMCLELLSDIDLKRKNALDVGCGSGILSIALKKQGVSALVACDTDSLAVEETLKNFSLNQIPLLVQDKVIYGSTQKIEGRFDVIVANLVADVIKSLYSEFVRLCNHTLILSGILETHLNSVLQIYYNGFEVLEQRQRNEWVALKLLKKQPIN
UniProt ID: O07678
Variant: DI -> NL at position 190-191
Variant annotation: in strain: NCTC 11638
Protein function: Methylates ribosomal protein L11.
Reference sequence:
MLKPMYYEFFFIFPKERELFESFLLDATHLALEESSLENLKAFDDKETIGFISQSNWHYFATHDPLKKDLKENLKEKPPHLKNFVILRSQKDLNNSLIPALEAFCLNLKQNLQSEFDFFYLSRNLASKDWLEAYKQAILPVQCTKFYIHPSWHQKPSHVVTNDCIMIDPALAFGSGHHESTSMCLELLSDIDLKRKNALDVGCGSGILSIALKKQGVSALVACDTDSLAVEETLKNFSLNQIPLLVQDKVIYGSTQKIEGRFDVIVANLVADVIKSLYSEFVRLCNHTLILSGILETHLNSVLQIYYNGFEVLEQRQRNEWVALKLLKKQPIN
UniProt ID: O07678
Variant: I -> V at position 251-251
Variant annotation: in strain: NCTC 11638
Protein function: Methylates ribosomal protein L11.
Reference sequence:
MLKPMYYEFFFIFPKERELFESFLLDATHLALEESSLENLKAFDDKETIGFISQSNWHYFATHDPLKKDLKENLKEKPPHLKNFVILRSQKDLNNSLIPALEAFCLNLKQNLQSEFDFFYLSRNLASKDWLEAYKQAILPVQCTKFYIHPSWHQKPSHVVTNDCIMIDPALAFGSGHHESTSMCLELLSDIDLKRKNALDVGCGSGILSIALKKQGVSALVACDTDSLAVEETLKNFSLNQIPLLVQDKVIYGSTQKIEGRFDVIVANLVADVIKSLYSEFVRLCNHTLILSGILETHLNSVLQIYYNGFEVLEQRQRNEWVALKLLKKQPIN
UniProt ID: O07678
Variant: Q -> K at position 317-317
Variant annotation: in strain: NCTC 11638
Protein function: Methylates ribosomal protein L11.
Reference sequence:
MLKPMYYEFFFIFPKERELFESFLLDATHLALEESSLENLKAFDDKETIGFISQSNWHYFATHDPLKKDLKENLKEKPPHLKNFVILRSQKDLNNSLIPALEAFCLNLKQNLQSEFDFFYLSRNLASKDWLEAYKQAILPVQCTKFYIHPSWHQKPSHVVTNDCIMIDPALAFGSGHHESTSMCLELLSDIDLKRKNALDVGCGSGILSIALKKQGVSALVACDTDSLAVEETLKNFSLNQIPLLVQDKVIYGSTQKIEGRFDVIVANLVADVIKSLYSEFVRLCNHTLILSGILETHLNSVLQIYYNGFEVLEQRQRNEWVALKLLKKQPIN
UniProt ID: O08314
Variant: NN -> KH at position 10-11
Variant annotation: in strain: P1
Protein function: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Reference sequence:
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
UniProt ID: O08314
Variant: N -> D at position 18-18
Variant annotation: in strain: P1
Protein function: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Reference sequence:
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
UniProt ID: O08314
Variant: A -> V at position 46-46
Variant annotation: in strain: P1
Protein function: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Reference sequence:
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
UniProt ID: O08314
Variant: E -> G at position 48-48
Variant annotation: in strain: P1
Protein function: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Reference sequence:
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
UniProt ID: O08314
Variant: EE -> GQ at position 72-73
Variant annotation: in strain: P1
Protein function: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Reference sequence:
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
UniProt ID: O08314
Variant: G -> V at position 76-76
Variant annotation: in strain: P1
Protein function: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Reference sequence:
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
UniProt ID: O08314
Variant: R -> H at position 79-79
Variant annotation: in strain: P1
Protein function: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Reference sequence:
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
UniProt ID: O08314
Variant: Y -> Q at position 84-84
Variant annotation: in strain: P1
Protein function: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Reference sequence:
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
UniProt ID: O08314
Variant: D -> G at position 108-108
Variant annotation: in strain: P1
Protein function: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Reference sequence:
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
UniProt ID: O08314
Variant: SK -> NN at position 119-120
Variant annotation: in strain: P1
Protein function: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Reference sequence:
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
UniProt ID: O08314
Variant: M -> L at position 169-169
Variant annotation: in strain: P1
Protein function: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Reference sequence:
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
UniProt ID: O08314
Variant: K -> N at position 177-177
Variant annotation: in strain: P1
Protein function: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Reference sequence:
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
UniProt ID: O08314
Variant: S -> N at position 181-181
Variant annotation: in strain: P1
Protein function: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Reference sequence:
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH
UniProt ID: O08314
Variant: E -> K at position 206-206
Variant annotation: in strain: P1
Protein function: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Reference sequence:
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDAQEILGAKLILANTYHMYLRPGEKVVEELGGLHRFAQFYGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANMSLEYHKEKNRPSNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDKPRYLMGVGTPENILDAISLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDNTPIEENCACYACKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLEKYNSRSH