Biodegradation of petroleum hydrocarbons in the arctic by cold-adapted microorganisms - NRC Publications Archive - National Research Council Canada
ARCSACC Conference - Assessment and Remediation of Contaminated Sites in Arctic and Cold Climates, Edmonton, May 07-08, 2001
Purpose: Triple-negative breast cancer (TNBC) does not express estrogen receptor, progesterone receptor, or Her2/neu. Both diagnosis and treatment of TNBC remain a clinical challenge. LyP-1 is a cyclic 9 amino acid peptide that can bind to breast cancer cells. The goal of this study was to design and characterize LyP-1 conjugated to fluorescent iron oxide nanoparticles (LyP-1-Fe3O4-Cy5.5) as a contrast agent for improved and specific magnetic resonance imaging (MRI) in a preclinical model of TNBC. Procedures: The binding of LyP-1-Fe3O4-Cy5.5 to MDA-MB-231 TNBC cells was evaluated and compared to scrambled peptide bio-conjugated to iron oxide nanoparticles (Ctlpep-Fe3O4-Cy5.5) as a negative control. Following the in vitro study, the MDA-MB-231 cells were injected into mammary glands of nude mice. Mice were divided into two groups: control group received Ctlpep- Fe3O4-Cy5.5 and LyP-1 group received LyP-1-Fe3O4-Cy5.5 (tail vein injection at 2 mg/kg of Fe3O4). Mice were imaged with an in vivo fluorescence imager and a 9.4 T MRI system at various time points after contrast agent injection. The T2 relaxation time was measured to observe accumulation of the contrast agent in breast tumor and muscle for both targeted and non-targeted contrast agents. Results: Immunofluorescence revealed dense binding of the LyP-1-Fe3O4-Cy5.5 contrast agent to MDA-MB-231 cells; while little appreciable binding was observed to the scrambled negative control (Ctlpep-Fe3O4-Cy5.5). Optical imaging performed in tumor-bearing mice showed increased fluorescent signal in mammary gland of animals injected by LyP-1-Fe3O4-Cy5.5 but not Ctlpep- Fe3O4-Cy5.5. The results were confirmed ex vivo by the 2.6-fold increase of fluorescent signal from LyP-1-Fe3O4-Cy5.5 in extracted tumors when compared to the negative control. In MR imaging studies, there was a statistically significant (P < 0.01) difference in normalized T2 between healthy breast and tumor tissue at 1, 2, and 24 h post injection of the LyP-1-Fe3O4-Cy5.5. In animals injected with LyP-1-Fe3O4, distinct ring-like structures were observed with clear contrast between the tumor core and rim. Conclusion: The results demonstrate that LyP-1-Fe3O4 significantly improves MRI contrast of TNBC, hence has the potential to be exploited for the specific delivery of cancer therapeutics.
Get@NRC: The role of turbulence and magnetic fields in simulated filamentary structure (Opens in a new window)
We use numerical simulations of turbulent cluster-forming regions to study the nature of dense filamentary structures in star formation. Using four hydrodynamic and magnetohydrodynamic simulations chosen to match observations, we identify filaments in the resulting column density maps and analyze their properties. We calculate the radial column density profiles of the filaments every 0.05 Myr and fit the profiles with the modified isothermal and pressure-confined isothermal cylinder models, finding reasonable fits for either model. The filaments formed in the simulations have similar radial column density profiles to those observed. Magnetic fields provide additional pressure support to the filaments, making "puffier" filaments less prone to fragmentation than in the pure hydrodynamic case, which continue to condense at a slower rate. In the higher density simulations, the filaments grow faster through the increased importance of gravity. Not all of the filaments identified in the simulations will evolve to form stars: some expand and disperse. Given these different filament evolutionary paths, the trends in bulk filament width as a function of time, magnetic field strength, or density are weak, and all cases are reasonably consistent with the finding of a constant filament width in different star-forming regions. In the simulations, the mean FWHM lies between 0.06 and 0.26 pc for all times and initial conditions, with most lying between 0.1 to 0.15 pc; the range in FWHMs is however, larger than seen in typical Herschel analyses. Finally, the filaments display a wealth of substructure similar to the recent discovery of filament bundles in Taurus.