The eagle eventually collected all the 108 beads after decades of seeking. Still, the final time has not arriv
This sample version will allow you to try each of the 7 activities on your phone for free.The app is an Adv
pull up a seat for yourself, partner, and i’ll tell you a story. a story of a young lass came tearin’ out of the west with a storm in her heart and the desert in her eyes. she had something to prove, by molly, and hell if she didn’t prove it - pistols at high noon, the way young ones proved themselves those days. a lady after my own heart. went by the name of annie - calamity annie, and you’d know why if you’d been fool enough to get in her way. this game, calamity annie, was started in texas and finished in new york. annie’s got long legs. the graphics were done in graphicsgale, the music in organya twei, and i put it all together in game maker: meaning that - at least for now - the game needs a windows computer with directx to run. you’ll also need a mouse, since the game is entirely played with it. your mouse is your gun.
This is a past event. If you missed it or would like to review the presentations given, please check out this archival video we shot at the event. https://youtu.be/UZ9BTMV5I8k The new California Desert Arts Council is up and running with an awesome website launch a few weeks ago and now their first Desert Arts Salon event. Wednesday, May 17, from 5:30 to 7pm CREATE Center for the Arts 73446 California 111 Palm Desert, CA, 92260 (111 at San Pablo [more...]
High-throughput screening identifies novel inhibitors of the acetyltransferase activity of Escherichia coli GlmU — Brown Lab
High-throughput screening identifies novel inhibitors of the acetyltransferase activity of Escherichia coli GlmU
The bifunctional GlmU protein catalyzes the formation of UDP-N-acetylglucosamine in a two-step reaction using the substrates glucosamine-1-phosphate, acetyl coenzyme A, and UTP. This metabolite is a common precursor to the synthesis of bacterial cell surface carbohydrate polymers, such as peptidoglycan, lipopolysaccharide, and wall teichoic acid that are involved in the maintenance of cell shape, permeability, and virulence. The C-terminal acetyltransferase domain of GlmU exhibits structural and mechanistic features unique to bacterial UDP-N-acetylglucosamine synthases, making it an excellent target for antibacterial design. In the work described here, we have developed an absorbance-based assay to screen diverse chemical libraries in high throughput for inhibitors to the acetyltransferase reaction of Escherichia coli GlmU. The primary screen of 50,000 drug-like small molecules identified 63 hits, 37 of which were specific to acetyltransferase activity of GlmU. Secondary screening and mode-of-inhibition studies identified potent inhibitors where compound binding within the acetyltransferase active site was requisite on the presence of glucosamine-1-phosphate and were competitive with the substrate acetyl coenzyme A. These molecules may represent novel chemical scaffolds for future antimicrobial drug discovery. In addition, this work outlines the utility of catalytic variants in targeting specific activities of bifunctional enzymes in high-throughput screens.
I’ve been thinking about going back to the list of 2016 goals I published earlier this year and check-in on how I’m doing against them. This past Saturday I was really pleased to read Yvonne’s (Quilting Jet Girl) mid-year review post for two reasons. The first was that it was a very interesting, well thought-out …
Half of the year gone! Whew! It is time to set goals for Q3. 1. My quilt from my ABQ MQG challenge and my Victoria Findlay Wolfe class. Most of the quilting is done for this one, so on to binding and making the label. 2. Red and White stars – I made this quilt …
Another month, time for more blocks! I’m a little late in posting the Splendid blocks I finished in June. I have this problem that I keep thinking “I’ll just finish one more block and add it to the post!” But as it is mid-July, I think the time has come to post the June blocks. …
Posted on July 11, 2016Categories New Bloggers Group50 Comments on Welcome my stop on the New Bloggers Blog Hop
Hello, and welcome to my stop on the 2016 New Bloggers Blog Hop! It’s been a fun few weeks learning about all the fabulous folks in our group, and now it is my turn! Thanks so much to Cheryl (Meadow Mist Designs) for hosting my group of new bloggers – Miss Bee-hivin’! Also thanks to Yvonne …