2020-21 Pohár FAČR
İkinci tur

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| 13 Eylül 2020 | SFK Vrchovina | 1-2 | FC Vysočina Jihlava | Nové Město na Moravě |
16:00 OAYS: 
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| 15 Eylül 2020 | MFK Frýdek-Místek | 1-2 | FK Blansko | Frýdek-Místek |
16:00 OAYS: 
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| 16 Eylül 2020 | TJ Jiskra Domažlice | 2-0 | FK Ústí nad Labem | Domažlice |
16:00 OAYS: 
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| 16 Eylül 2020 | FC Slovan Velvary | 0-2 | Bohemians 1905 | Velvary |
16:00 OAYS: 
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| 16 Eylül 2020 | Sokol Hostouň | 0-1 | FK Mladá Boleslav | Hostouň |
16:00 OAYS: 
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| 16 Eylül 2020 | SK Vysoké Mýto | 1-2 | FK Přepeře | Vysoké Mýto |
16:00 OAYS: 
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| 16 Eylül 2020 | TJ Jiskra Ústí nad Orlicí | 1-0 | FC Fastav Zlín | Ústí nad Orlicí |
16:00 OAYS: 
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| 16 Eylül 2020 | TJ Unie Hlubina | 0-6 | MFK Karviná | Ostrava |
16:00 OAYS: 
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| 16 Eylül 2020 | SK Beskyd Frenštát pod Radhoštěm | 0-4 | FC Baník Ostrava | Frenštát pod Radhoštěm |
16:00 OAYS: 
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| iptal | Povltavská fotbalová akademie | 0-3 | FC MAS Táborsko |  |

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| iptal | TJ Sokol Srbice | 0-3 | FC Sellier & Bellot Vlašim |  |

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| iptal | FK Motorlet Prague | 0-3 | MFK Chrudim |  |

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| iptal | FK Baník Most - Souš | 0-3 | SK Dynamo České Budějovice |  |

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| iptal | SK Benešov | 0-3 | FK Teplice |  |

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| iptal | FK Kolín | 0-3 | FK Viktoria Žižkov |  |

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| iptal | Loko Vltavín | 0-3 | 1. FK Příbram |  |

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| iptal | SK Aritma Prague | 0-3 | FK Dukla Prague |  |

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| iptal | FK Olympie Březová | 0-3 | FC Hradec Králové |  |

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| iptal | SK Český Brod | 0-3 | FK Chlumec nad Cidlinou |  |

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| iptal | FC Velké Meziříčí | 0-3 | 1. SK Prostějov |  |

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| iptal | SK Hanácká Slavia Kroměříž | 0-3 | SFC Opava |  |

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| iptal | FC Odra Petřkovice | 0-3 | SK Sigma Olomouc |  |

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| iptal | 1. SC Znojmo FK | 0-3 | SK Líšeň |  |

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| iptal | FC Hlučín | 0-3 | FK Fotbal Třinec |  |

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| iptal | FC Slovan Rosice | 0-3 | FC Zbrojovka Brno |  |

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| iptal | MFK Vyškov | 0-3 | 1. FC Slovácko |  |

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| iptal | FC Slavia Karlovy Vary | 3-0 | FC Slavoj Vyšehrad |  |