= 9*z - d - 0. Sort z, 1, 0, 2 in ascending order.
0, 1, 2, z
Let v be (-12)/20 - (-4)/6. Let m be (-20)/8*9/15. Put m, v, 2/13 in increasing order.
m, v, 2/13
Let f(x) = -2*x**2 - 31*x - 61. Let k be f(-14). Sort -4, -9, k, -1 in decreasing order.
-1, -4, -9, k
Let d = -96 + 105. Suppose 0 = -d*f + 25*f - 64. Put -5, 5, f, -27 in increasing order.
-27, -5, f, 5
Let w = 43/252 - 1/36. Let k = -0.2 + -0.3. Sort k, 4, w in decreasing order.
4, w, k
Suppose g - 1 = 6. Let x(o) = o**3 - 6*o**2 - 5*o - 10. Let i be x(g). Sort -2, i, 2 in decreasing order.
i, 2,detected the translocation of β-Catenin into the nucleus was impaired, meaning the inhibition of the Wnt/β-Catenin pathway. Taken together, we identified a positive correlation between up-regulation of nucleostemin and human glioma cell proliferation and that knocking-down nucleostemin alleviated glioma proliferation by reducing β-Catenin transportation into the nucleus. All results suggested that nucleostemin might accelerate human glioma proliferation via the Wnt/β-Catenin pathway.


the squib resistor (4) will generate heat to trigger the expansion of the air bag to protect the driver.
In the prior art fault detector, it has been necessary to set the gain of the DC differential amplifier circuit (7) to 100 or so in order to detect a voltage variation being caused by any fault. Therefore, the fault detector of the prior art has such problems as being weak for noise because of the high gain in amplification, which causes errors due to the input offset voltage of the amplifier if a DC differential amplifier is utilized and, moreover, it is necessary to install a precision amplifier which requires precise adjustments in the stages of production, hence, resulting in the loss of the reliability as well as increasinginflate process
 * and document some of the less obvious aspects of the format.  This source
 * code is meant to supplement RFC 1951, which formally describes the deflate
 * format:
 *
 *    http://www.zlib.org/rfc-deflate.html
 */

/*
 * Change history:
 *
 * 1.0  10 Feb 2002     - First version
 * 1.1  17 Feb 2002     - Clarifications of some comments and notes
 *                      - Update puff() dest and source pointers on negative
 *


                     uncompressed data
 * 1.3  20 Mar 2002     - Go back to lengths for puff() parameters [Jean-loup]
 *                      - Add a puff.h file for the interface
 *                      - Add braces in puff() for else do [Jean-loup]
 *                      - Use indexesint32_t len;            /* current number of bits in code */
    int32_t code;           /* len bits being decoded */
    int32_t first;          /* first code of length len */
    int32_t count;          /* number of codes of length len */
    int32_t index;          /* index of first code of length len in symbol table */
    int32_t bitbuf;         /* bits from stream


should not use memcpy() since its behavior is not
 *   defined for overlapped arrays.  You should not use memmove() or bcopy()
 *   since though their behavior -is- defined for overlapping arrays, it is
 *   defined to do the wrong thing in this case.
 */
local int32_t codes(struct state *s,
                struct huffman *lencode,
                struct huffman *distcode)
{
    int32_t symbol;         /* decoded symbol */
    int32_t len;            /* length for copyby the format.  So incomplete
 *   literal/length codes of one symbol should also be permitted.
 *
 * - If there are only literal codes and no lengths, then there are no distance
 *   codes.  This is represented by one distance code with zero bits.
 *
 * - The list of up to 286 length/literal lengths and up to 30 distance lengths
 *   are themselves compressed using Huffman codes and run-length encoding.  In
 *   the list of code lengths, a 0 symbol means no code, a 1..15 symbol means
 *   that length, and the symbols 16, 17, and 18 are run-length instructions.
 *   Each of 16, 17, and 18 are follwed by extra bits


to define the length of
 *   the run.  16 copies the last length 3 to 6 times.  17 represents 3 to 10
 *   zero lengths, and 18 represents 11 to 138 zero lengths.  Unused symbols
 *   are common, hence the special coding for zero lengths.
 *
 * - The symbols for 0..18 are Huffman coded, and so that code must be
 *   described first.  This is simply a sequence of up to 19 three-bit values
 *   representing no code (0) or the code length for that symbol (1..7).
 *
 * - A dynamic block starts with three fixed-size counts from which is computed
 *   the number of literal/length code lengths, the numberit was a fixed or dynamic block) are undefined and have no
 *   expected values to check.
 */
int32_t puff(uint8_t  *dest,           /* pointer to destination pointer */
             uint32_t *destlen,        /* amount of output space */
             uint8_t  *source,         /* pointer to source data pointer */
             uint32_t *sourcelen)      /* amount of input available */
{
    struct state s;


= v + 1. Let u(d) = -2*d - 2. Let o(b) = -7*b**2 - 72*b - 125. Let z be o(-8). Give z*l(k) + u(k).
k + 1
Let i(l) = -10*l**2 + 681 - 1357 + 674 - 4*l. Let j = -15 + 21. Let h(k) = 11*k**2 + 5*k + 2. Calculate j*i(q) + 5*h(q).
-5*q**2 + q - 2
Let n(o) = 6*o - 42. Let y(p) = -2*p + 14. Let w(v) = 5*n(v) + 14*y(v). Let f(d) = 1. Suppose 140*h + 77*h - 108 = 109*h. Calculate h*w(k) + f(k).
2*k - 13
Let p(n) = 109*n**3 + 15*n**2 + 7*n - 9. Let k(t) = -112*t**3 - 18*t**2 - 8*t + 10. Calculate -5*k(r) - 6*p(r).
-94*r**3 - 2*r + 4
Let s(q) = 10252*q + 2.same as males for triglycerides. The only other difference between the sexes was the second highest loading for PC3 was low glucose-to-insulin ratio in males versus low ferritin for females ([Table 2](#pone.0218763.t002){ref-type="table"}, Figs [1](#pone.0218763.g001){ref-type="fig"} and [2](#pone.0218763.g002){ref-type="fig"}).

While the bivariate and multivariate correlation and principal component structures were very similar in both sexes, there was no significant correlation between the measures of heart disease or age in females ([Table 3](#pone.0218763.t003){ref-type="table"}). Although the age distributions of both males and females were very similar, the heart thicknesses were significantly smaller in females, (Figs [1](#pone.0218763.g001){ref-type="fig"} and [2](#pone.0218763.g002){ref-type="fig"}); therefore, females simply have not progressed as far as males out on the first component, despite equivalent ages. That is, the primary dimensions of risk of heart disease are similar in both sexes, however for all


in humans. Studies in human health identify hyperlipidemia as a risk factor in cardiovascular disease \[[@pone.0218763.ref018]\]. Other studies have examined the influence of the gut microbiota on cholesterol metabolism \[[@pone.0218763.ref019]\]. The gut microbiota are also shown to have significant effect on cardiovascular health \[[@pone.0218763.ref020]\]. We previously demonstrated that the fecal microbiome of male gorillas with cardiac disease differs from that of healthy males \[[@pone.0218763.ref021]\]. A future avenue of investigation will focus on the role of the gorilla gut microbiome in cholesterol metabolism in association with heart disease.

Supporting information {#sec009}
======================

###### Raw data.

All data used for the present analyses are included here. For the column labeled sex, F = female, M = male. Age is provide in years. Units for serum markers and heart measures are provided in [Table 1](#pone.0218763.t001){ref-type="table"}               .thenReturn(2L);

        Object result = sendHeaders(
                RedisConstants.COMMAND, "LINSERT",
                RedisConstants.KEY, "key",
                RedisConstants.VALUE, "value",
                RedisConstants.PIVOT, "pivot",
                RedisConstants.POSITION, "BEFORE");

        verify(listOperations).leftPush("key", "pivot", "value");


VermilionCity_PokemonCenter_2F_MapScripts:: @ 816B457
	map_script MAP_SCRIPT_ON_FRAME_TABLE, CableClub_OnFrame
	map_script MAP_SCRIPT_ON_WARP_INTO_MAP_TABLE, CableClub_OnWarp
	map_script MAP_SCRIPT_ON_LOAD, CableClub_OnLoad
	map_script MAP_SCRIPT_ON_TRANSITION, CableClub_OnTransition
	.byte 0

@ The below 3 are unused and leftover from RS
VermilionCity_PokemonCenter_2F_EventScript_Colosseum:: @ 816B46C
	call CableClub_EventScript_Colosseum
	end

VermilionCity_PokemonCenter_2F_EventScript_TradeCenter:: @ 816B472
	call CableClub_EventScript_TradeCenter
	end

VermilionCity_PokemonCenter_2F_EventScript_RecordCorner:: @ 816B478
	call CableClub_EventScript_RecordCorner
	end
making agriculture more profitable, providing access to financial services, improving security of land tenure, and educating its youth with advanced skills and knowledge. Only if African leaders tackle these challenges will they reach their full potential.

While public investment in agriculture is essential for increasing agricultural growth in Africa, it must be complemented by good policy in order to be effective and efficient. A new IFPRI discussion paper examines a variety of agricultural policies that have been implemented in Africa south of the Sahara and identifies which have been most effective in stimulating agricultural growth.

Historically, the agriculture sector in Africa has suffered from inefficient market interventions, volatile food prices, and severe underinvestment. A variety of policies have been implemented in order to improve agricultural productivity, encourage private-sector investment, stabilize


absurd and ridiculous” to them, King notes.

“Did you ever wonder how it is we imagine the world in the way we do, how it is we imagine ourselves, if not through our stories? And in the English-speaking world, nothing could be easier, for we are surrounded by stories, and we can trace these stories back to other stories,” King writes.

For example, we could go back to famous literature like James Fenimore Cooper’s novel, The Deerslayer:

According to Deerslayer, revenge is an Indian gift and forgiveness is a White gift. Indians have devious natures, while Whites believe the best of a person. In the end, all Cooper is doing here is reiterating the basic propagandas that the British would use to justify their subjugation of Indian, or that the Germans
Fool Britannia: the future of the UK has never looked so grim - fanf2
https://www.theglobeandmail.com/opinion/fool-britannia-brexit-uk-great-britain/article37137569/
======
tim333
Slightly on the positive there was a poll published last weekend in the Mail
of all places saying

"And there is clear support for a second referendum on the final Brexit deal.
One in two say there should be a second vote when all the talking is done,
against 34 per cent who are opposed."

I mean if we can see what is actually on the table and the majority of voters
still want it then I guess fair enough. It seems iffy though to ram Brexit
through regardless of how bad it is on the basis of an ill informed vote a
while back.

~~~
T-A
I find it hard to conjure up a realistic scenario where there is enough time
to hold a second referendum


really they
should be looking at the damage the Tories have done to the UK.

~~~
Boothroid
Remain too. We were told that on the day after a vote to leave there would be
an immediate crash of financial markets and recession. That didn't happen.

~~~
boyce
Sensible heads weren't predicting an instant crash but greatly reduced growth
and loss of value of the currency.

If you're cherry picking daft predictions there were plenty on each side.

~~~
Boothroid
The Treasury's document today says: "A vote to leave would cause an immediate
and profound economic shock creating instability and uncertainty which would
be compounded by the complex and interdependent negotiations that would
follow".

[http://www.independent.co.uk/news/business/news/brexit-to-
cr...](http://www.independent.co.uk/news/business/news/brexit-to-create-
instant-diy-recession-warns-george-osborne-a7042886.html)

~~~
rlpb
That did happen, didn't it?

No "immediate crash of financial markets and recession", but it doesn't appear
that they predicted that.

~~~
Boothroid
Why don't you try to refute my general point? There was FUD on bothOISD does not condone negative comments or actions toward any race,” the statement read. “The District values every individual and therefore the District will take the appropriate measures to address the situation expeditiously and completely.”

--Field Level Media


"strongly encouraged" players to join the Fellowship of Christian Athletes and attend Bible studies hosted by the group.

On March 24, Mendieta sent Killingsworth a letter voicing concerns about the mandatory church attendance and stopped attending Calvary.

The lawsuit says Killingsworth told her that she didn't care which church she went to. But it says the coach started to avoid speaking with Mendieta and became hostile.

In the spring of 2014, another player's parents complained about the coach's emphasis on religion, and that player ended up quitting the team, the lawsuit says. Though Killingsworth made church and Bible studies optional again, she continued to put church on the team schedule and treated players who attended better than those who didn't, the suit claims.

In the fall of 2014, Killingsworth began looking forUniversity of Florida collections, and the State of Florida's Division of Plant Industry collections.

The McGuire Center for Lepidoptera and Biodiversity serves both research and public education functions. The center includes the living Butterfly Rainforest and exhibit space that features information about Lepidoptera and rain forests worldwide, as well as  of research laboratories and collection space.

The research space includes laboratories focusing on molecular genetics, scanning electron microscopy, image analysis, conservation and captive propagation of endangered species, optical microscopy, and specimen preparation, as well as classrooms and offices for 12 faculty curators, collection managers, and other staff. Some of the research laboratories and collections can be viewed through glass panels at the back of the museum.

Public exhibits

Butterfly Rainforest

The Butterfly Rainforest is a display of live butterflies in a


that’s it.

We have to admit by being a bit darkly amused by the signs on the walls of the hospital proclaiming it lacks an A&E department – oh dear!

And finally, while we can’t condone graffiti and the like, one simple piece, written on the pavement by someone sat on the bench directly next to the spot of the Fall, did stand out in a simple and almost poetic way.

It sort of summed up the entire experience the area around St Bart’s now puts on you having watched The Reichenbach Fall, and it’s a definite proof of evidence of just how impactful these characters have become in our lives, and most likely yours if you are reading this article. It’s a wonderfully quiet area of London, divorced from thethe Cockpit,” which aims to provide protected, classified data connectivity. Other requests for funding survivability upgrades will likely emerge in the next five to 10 years, once AMC formalizes its requirements, Everhart said.

KC-Y Competition Still Under Consideration as Air Force Works to Define Future Tanker Fleet Air Mobility Command head Gen. Carlton Everhart had floated the idea of moving straight from the KC-46 program into the acquisition of a more technologically advanced KC-Z — a plan that would involve skipping a KC-Y competition and perhaps buying an upgraded version of the KC-46 to “bridge” the gap until KC-Z procurement can begin.

Going forward, the command will also assess whether its newest platform, the KC-46 tanker due for delivery this year, could benefit from enhancements — including making it more


on 11/04/2020 - 09:56Last edited 11/04/2020 - 11:13 by 2 other users

Recently saw this flyer in Richmond Melbourne , and took part in the service yesterday and recommended

$15 - 2 Knives

$20 - 3 Knives

$10 - Pick up and collection service

Current Suburbs - Richmond, Hawthorn, South Yarra, CBD, Carlton, Fitzroy, Collingwood

Joe - 0435 199 604

He's looking to get other unemployed chefs onboard to expand the areado not have "form" in this area. They agreed to the expenditure of some £300,000 of Cycle Safety Fund money on the Bedford "turbo-roundabout" scheme, which was from the very start intended only to reduce collisions between motor vehicles arising from high volumes of traffic entering the roundabout at high speeds. Cyclists are not supposed to use the roundabout itself - they have been offered shared use of existing footpaths around the perimeter!What is, in my mind, a victory, is that we have apparently prevented such misuse of funds. The £1.5m can now be released to be re-applied to another national park (South Downs seems to spend the money more wisely) or to a city scheme. That is second-best to the schemes intended for the New Forest, but


so I'm not going to go into all of your options. There are a lot out there. You should consider using one of the existing licenses rather than inventing your own. Existing licenses have been carefully written and (usually) reviewed by people with a legal background - these are things that are more likely to stand up when challenged.

Once you choose a license, you just make it obvious which one you have. Usually, this includes including a LICENSE file in your release that includes the full text of the licenses. Often, individual source files will also contain a line that identifies the copyright holder and the license under which the copyright holder has released the code.

If the school is contracting you to creating this application, then it isQ:

Does changing the Windows Server 2008 "Region and language" > Format setting have any impact on a SQL Server 2008 R2 database engine installed on it?

I've found at MSDN that

The database engine doesn't care about regional settings

But I haven't found any documentation supporting this statement. For example, would it change the way it displays (or the way I edit) date, time or currencies in SSMS?
It seems like it won't, since dates in the database are totally different to the sample dates shown in the Formats tab. But, I would like to have more reading on this before having to restart my server many times (which is expensive for me).

A:

The general answer is no, indeed, as stated in MSDN.
More precisely, would it change the way it displays (or the


be useful in chronic kidney disease. For example, radix salviae miltiorrhiza was proved to be able to reverse the transdifferentiation of human renal proximal tubular epithelial cells induced by TGF-*β*1, thus delaying the procession of renal fibrosis in injured kidneys \[[@B18]\]. Rhubarb could alleviate tubulointerstitial fibrosis by inhibiting TGF-*β*/Smad pathway in chronic kidney disease \[[@B19]\]. Rhubarb also could suppress tubulointerstitial fibrosis in 5/6 nephrectomy rats by alleviating IS overload and reducing kidney oxidative stress and inflammatory injury \[[@B20]\]. Radix angelicae sinensis is good for preventing and treating renal tubulointerstitial fibrosis with effect similar to the angiotensin converting enzyme inhibitor (ACEI) and angiotensin receptor blocker (ARB). Its mechanism was proved to be able to improve renal fibrosis by downregulating of TGF-*β*/Smad signaling pathway and lowering the expression of angiotensin53, provides that no ordinance or resolution of a municipality shall become operative until thirty days after its passage and approval unless it is passed over the veto of the executive officer, or unless it carries an emergency clause in a separate section adopted by a three-fourths vote of all members elected to the council taken by ayes and noes. Ordinance No. 4475 did not contain such a clause when it was enacted and approved on June 9th; and it has been expressly held by the Supreme Court of the state that the subsequent action of the council in attempting to fix it was a nullity, that the ordinance was subject to referendum, and that the petition for referendum was seasonably filed. State v. Pulliam, 168 Okl. 632,


fact that the city adopted the charter form of government in accordance with article 18, section 3(a), of the Constitution, and that article 5, section 10, of the charter provides that all general elections shall be held on the first Tuesday in April of each odd-numbered year. Pitts v. Allen, 138 Okl. 295, 281 P. 126; Perrine v. Bonaparte, 140 Okl. 165, 282 P. 332; City of Ardmore v. Excise Board of Carter County, 155 Okl. 126, 8 P.2d 2.
Disposition was made of the case upon the pleadings. No evidence was submitted respecting the submission of the referred question. Defendants assert that it was not submitted at the general municipal election held in April, 1935. Plaintiff does not controvert the statement. Instead, she asserts that the trial courtoil and gas and from the production of such minerals. The bill and the amended bill and supplement bill charge, among other things, that the production of oil and gas will wrongfully subject her property to the constant and continuing danger of destruction by explosion and fire; that such danger will destroy the value of the property for the purpose of sale or rental; and that it will be rendered worthless. The controversy is not moot in respect to these questions. It continues so far as they are concerned and the court is empowered to determine them with a subject matter upon which the decree could operate effectually. A case will not be dismissed where only a part of the controversy has become moot and other questions remain


to live to see another day. Level 1: You gain a +1 to attack and damage rolls made with this weapon. Bind. When you attack a creature with this weapon you can activate this property of the weapon. They must succeed on a DC 15 Dexterity saving throw or else be lashed to the weapon by a rope of crackling energy. If a creature succeeds, they are immune to this effect for 1 round. Whilst lashed the creature cannot use the Disengage action, and when they provoke opportunity attacks from you they have their speed reduced to 0 on a successful hit. Whilst bound to the weapon, creatures take 1d8 lightning damage at the start of their turns. You can be lashed to a maximum of three creaturesweapons

bludgeoning, piercing and slashing damage from nonmagical weapons Damage Immunities poison

poison Condition Immunities poisoned

poisoned Senses darkvision 120ft., passive Perception 20

darkvision 120ft., passive Perception 20 Languages Abyssal, Common, Infernal, telepathy 120ft. Magic Resistance. Betray has advantage on saving throws against spells and other magical effects. Magic Weapons. Betray's weapon attacks are magical. Ravage. Betray crits on a 17. When it reduces an enemy to 0 hit points with a crit it can immediately use its Deceive action without using an action and make a single attack. If this attack hits, it is automatically a crit. Actions Multiattack. Betray makes two attacks with its dagger. Dagger. Melee Weapon Attack: +14 to hit, reach 5ft., one target. Hit: 19 (3d6 + 9) slashing damage. In addition, targets hit by this attack


poisoned, prone, restrained Senses darkvision 120ft., passive Perception 19

darkvision 120ft., passive Perception 19 Languages Abyssal, Common, Infernal, telepathy 120ft. Magic Resistance. Hinder has advantage on saving throws against spells and other magical effects. Magic Weapons. Hinder's weapon attacks are magical. Impede. When a creature friendly to Hinder is attacked within 30 ft of it, Hinder can use its reaction to interpose itself between the creature and its assailants, taking all the attacks directed at the creature. This movement does not provoke opportunity attacks and Hinder gains a +2 bonus to its AC until the start of its next turn. Actions Multiattack. Hinder makes two shortsword attacks. Shortsword. Melee Weapon Attack: +12 to hit, reach 5ft., one target. Hit 17 (3d6 + 7) slashing damage. Disrupt. Hinder can projectCon +10, Int +7, Wis +10, Cha +7

Str +11, Dex +8, Con +10, Int +7, Wis +10, Cha +7 Skills Athletics +16, Intimidation +12, Perception +10

Athletics +16, Intimidation +12, Perception +10 Damage Resistances bludgeoning, piercing and slashing damage

bludgeoning, piercing and slashing damage Damage Immunities poison, psychic

poison, psychic Condition Immunities charmed, frightened, poisoned

charmed, frightened, poisoned Senses darkvision 120ft., passive Perception 20

darkvision 120ft., passive Perception 20 Languages Abyssal, Common, Infernal, telepathy 120ft. Magic Resistance. Scorn has advantage on saving throws against spells and other magical effects. Magic Weapons. Scorn's weapon attacks are magical. Actions Multiattack. Scorn makes three greataxe attacks. Greataxe. Melee Weapon Attack: +14 to hit, reach 5ft., one target. Hit 22 (3d8 + 9) slashing damage. In addition, targets hit by this attack take 11 (2d10) psychic damage.


reviewed and extracted independently by two authors. A random effects model was used to estimate the pooled prevalence. Sources of heterogeneity among the studies were determined using subgroup analysis and meta-regression. In total, 1817 records were identified in the initial search, and 46 records were included in the meta-analysis. The overall prevalence of HCV among high-risk groups was 32.3%. The prevalence was 41.3% in injection drug users (IDUs), 22.9% in prisoners, 16.2% in drug-dependent individuals and 24.6% in drug-dependent prisoners. Subgroup and meta-regression analyses revealed that geographical location and year of publication were the probable sources of heterogeneity. This meta-analysis found a high prevalence of HCV among high-risk groups in Iran, particularly among IDUs. There is a need for prevention strategies to reduce the burden of HCV infectionNarendra Modi to give first speech after Uri attack – Live streaming & live telecast Also Read - PM Modi to Interact With Fitness Influencers to Celebrate One Year of 'Fit India Movement' on Thursday

Subramanian Swamy has been known for making provocative statements and he is also dubbed as a senseless speaker. Swamy’s venomous comments cost him his job at Harvard, where he was appointed as a professor once. Subramanian Swami faced unprecedented anger by Harvard University after his article in Indian newspaper demonised Muslims. Also, 400 students from Harvard complained about Swamy’s behaviour at the university and asked the administration to remove him. Also Read - PM Modi to Stock of COVID-19 Situation, Chair High-Level Meeting With CMs of 7 Worst-hit States Today

Prime Minister Narendra Modi meets


architectural and allometrical regularities of real trees. These scaling laws were compared to three-dimensional FEM modal analyses of two completely digitized trees with maximal architectural contrast. Despite their simplifying hypotheses, the models explained most of the spatiotemporal characteristics of modes that involved the trunk and branches, especially for sympodial trees. These scaling laws reduce the tree to (1) a fundamental frequency and (2) one architectural and three biometrical parameters. They also give quantitative insights into the possible biological control of wind excitability of trees through architecture and allometries.Impact of hypertension on cerebral microvascular structure in CPAP-treated obstructive sleep apnoea patients: a diffusion magnetic resonance imaging study.
Obstructive sleep apnoea (OSA) is a highly prevalent sleep-related breathing disorder associated with hypertension, impaired peripheral vascular function and an increased risk of stroke. Evidence suggests that abnormalities of the cerebral microcirculation, such as capillary rarefication, may be present in these patients. We evaluated whether the presence of hypertension may affect the cerebral capillary architecture and function assessed by Intravoxel Incoherent Motion (IVIM) magnetic resonance imaging (MRI) in patients with continuous positive airway pressure (CPAP)-treated OSA. Forty-one patients (88% male, mean age 57 ± 10 years) with moderate-to-severe OSA were selected and divided into two groups (normotensive vs. hypertensive). All hypertensive OSA patients were adherent with their antihypertensive medication. Cerebral


microvascular structure was assessed in grey (GM) and white matter (WM) using an echo-planar diffusion imaging sequence with 14 different b values. A step-wise IVIM analysis algorithm was applied to compute true diffusion (D), perfusion fraction (f) and pseudo-diffusion (D*) values. Group comparisons were performed with the Wilcoxon-Mann-Whitney-Test. Regression analysis was adjusted for age. Diffusion- and perfusion-related indexes in middle-aged OSA normotensive patients were quantified in both tissue types (D [10-3 mm2/s]: GM = 0.83 ± 0.03; WM = 0.72 ± 0.03; f (%) GM = 0.09 ± 0.01; WM = 0.06 ± 0.01; D* [10-3 mm2/s]: GM = 7.72 ± 0.89; WM = 7.38 ± 0.98). In the examined tissue types, hypertension did not result in changes on the estimated MRI IVIM index values. Based on IVIMA primary object of this invention is to provide an automatic closing device for bottles of mineral water to be fitted on to the bottle after the original metal crown cap with which it was sold has been removed.
At present various tops and caps for temporarily closing a bottle whilst the drink is being consumed are available.
For instance, small flexible plastic caps can be found which are simply pushed externally over the mouth of the bottle. These caps are attached by a strip to a ring which is slipped over the neck of the bottle.
Other types made of metal or china with rubber seals are held in place by clips or levers which fit round the neck of the bottle.
This invention has for its object to provide a


that this infographic can help seal the deal on helping you decide which phone is best for you.

If you haven’t read our review of Verizon’s HTC Droid Incredible, it’s worth a read. We were lucky enough to pick up a HTC EVO 4G at Google I/O 2010 and although our full in-dpeth review isn’t yet published, we have posted some great high-resolution product shots.

UPDATE: EVO 4G vs. iPhone 4 vs. Droid X Infographic here!

Both phones are for the most part similarly matched. While the HTC EVO has an edge due to its 4G compatibility, coverage is limited to just 32 cities at the moment. While the EVO’s large screen is a plus, it might be a bit bulky for some. The Droid Incredible offers a competitive advantage withit found that Oregon had the most dispensaries per capita with 16.5 cannabis businesses per 100,000 people. But Oklahoma was second with 15.6 dispensaries for the same sample.

Ray: Well that’s right. In fact, other Oklahoma cities ranked high in the number of dispensaries per capita. Norman was No. 7 with 14.1 dispensaries per 50,000 residents. Altogether, nine cities in Oklahoma were in the top 30 per capita. And so by all accounts, this surpassed all expectations for the industry in the first year.

Hutchinson: When compared to other states with similar laws, Oklahoma’s medical marijuana practices are somewhat lax. We mentioned before that Oklahoma doesn’t limit the number of licenses it issues for cannabis dispensaries. The state also doesn’t specify which medical conditions can qualify citizens for a card


Q:

Limit on Number of Google Spanner Read-Only Replicas

Spanner's documentation indicates that there are two read-write replicas per read-write region in a multi-region configuration of Spanner. Is there a limit (compute aside) on the number of read-only replicas per read-only region?

A:

Cloud Spanner does not have a published limit on the number of read-only replicas (either per-region or within an instance configuration as a whole).
However, note that when you create an instance, you select one of the existing instance configurations -- you can not define your own custom replication topology that pieces together different numbers of read-only replicas.

these methods still suffer from an inability to detect small amounts of LOH. Moreover, such techniques have still been limited to investigating a particular locus that is known to occur in a specific type of cancer. Thus, a screening for cancer in general has not been possible or effective.
Besides screening for an existence of cancer, current techniques are also lacking for providing a prognosis of a patient with cancer and for monitoring the effects of treatment (e.g. recovery after surgery or chemotherapy or immunotherapy or targeted therapy). Such techniques are often expensive (e.g. imaging techniques), inaccurate, ineffective, insensitive or may subject the patient to the radiation used for imaging techniques.
Accordingly, it is desirable to provide new techniques for screening, prognosticating, and monitoring a patient for cancer.


in first-principles TBMD methods, and their implementation in the [Fireball]{} code [@Lewis01; @Demkov95; @Sankey89]. The approximations used so far to handle these contributions are analyzed in Section II. In Section III we present our new approach to calculate the exchange-correlation contributions, and in Section IV we present some results for several materials to illustrate the performance of the new approach.

Ab initio tight binding: Fireball
=================================

[Fireball]{} [@Lewis01; @Demkov95; @Sankey89] is a first-principles TBMD simulation technique based on a self-consistent version of the Harris-Foulkes [@Harris85; @Foulkes89] functional. The energy functional is written as: $$E_{TOT} [ \rho(\vec r) ] = \sum_n\varepsilon_n-E_{ee}[\rho(\vec r)]
+E_{xc}[\rho(\vec r)] -
\int {\rho(\vec r)V_{xc}[\rho(\vec r)] d^3 r}
+ E_{ion-ion},
\label{Harris}$$ where $\rho(\vec r)$ is the [*input density*]{}, which will be allowed to vary, and will be determined selfconsistently. The first term is apractice, the atomic densities $\rho_i$ $$\begin{aligned}
\rho_i({\vec r})=\sum_{lm} q_{ilm} |\phi_{ilm} (\vec r )|^2.
\label{rho_ilm}\end{aligned}$$ are approximated to be spherically symmetric around each atomic site $i$ ([*i.e.*]{} $q_{ilm} = q_{ilm'}$). Self-consistency is achieved by imposing that the output orbital charges [$q_\mu^{out}$]{} (obtained from the occupied eigenvectors $\psi_n$ of equation (\[Heff\])) and input orbital charges $q_{\mu}$ coincide (see ref. [@Demkov95] and [@Lewis02] for further details).

The remaining difficulty is the efficient calculation of exchange-correlation interactions within a first-principles TB scheme. One possibility is to use non-standard DFT and introduce the exchange-correlation energy and potential as a function of the orbital occupancies [@OO-LCAO; @FJ; @Pou]. In this paper, however, we opt for the more traditional approach in which exchange-correlation contributions are calculated as a functional of the electron density $\rho (\vec r)$. Within this line, two different


methods have been previously proposed for the practical calculation of exchange-correlation terms, using data-tables similar to those for the Hartree contributions. These two methods are:

Sankey-Niklewski approximation
------------------------------

The basic idea introduced by SN [@Sankey89] is to write down the non-linear in $\rho ({\vec r})$ exchange-correlation matrix elements in terms of matrix elements of $\rho ({\vec r}) $ . These later matrix elements are easily tabulated in data-tables no larger than two-dimensional, similar to those required for the Hartree terms.

Consider the matrix elements $< \phi_\mu | V_{xc} [\rho] | \phi_\nu>$ of the exchange-correlation potential. For each matrix element $< \phi_\mu | V_{xc} [\rho] | \phi_\nu>$, expand $V_{xc} [ \rho ]$ in a Taylor’s series $$V_{xc} [ \rho ] \simeq V_{xc} [ \bar{\rho}_{\mu\nu} ]
+ V'_{xc} [ \bar{\rho}_{\mu\nu} ] (
\rho -  \bar{\rho}_{\mu\nu}upload your data
  to AWS using the spare capacity of your existing Internet connection,
  then you should consider using Snowball.

However it looks like those are intended for either import or export, not both at once.  Would I need to do two separate jobs with the same snowball? Won't I still get charged for all those PUT/LIST requests anyway, to get the data onto the snowball?  They mention the $0.03/GB for data transfer, but didn't mention API requests.

A:

If you're copying data that is already in AWS to another region, Snowball doesn't seem like a good option.  Running sync from the command line of an EC2 instance within AWS seems right.
Have you tried configuring max_concurrent_requests? Possibly that will address the performance issue.  Also, this


{#cas14359-sec-0003}
---------------------------

In this study, we used a human OSCC cell line, GFP‐SAS,[9](#cas14359-bib-0009){ref-type="ref"} which can form tumors when injected into athymic nude mice. Cells were maintained in DMEM (Wako) supplemented with 10% FBS (BioSource), 100 U/mL penicillin, and 100 µg/mL streptomycin (Wako), referred to here as complete medium, and grown in an incubator with a humidified atmosphere of 95% air and 5% CO~2~ at 37°C.

2.2. Functional screening of miRNA knockdown library {#cas14359-sec-0004}
----------------------------------------------------

To identify novel oncomiRs in human OSCC cells, the miRCURY LNA microRNA Knockdown Library -- Human version 12.0 (Exiqon) was used. We then transfected 918 locked nucleic acid (LNA)/DNA antisense oligonucleotides (ASOs) for specific human mature miRNAs into GFP‐SAS cells. Cells (2 × 10^3^/well) were then seeded into a 96‐well plate in complete medium with 25 nmol/L of each ASO and Lipofectamine RNAiMAX (Thermowas carried out in a 10 µL final reaction volume containing 5 µL of 2× QuantiTect SYBR Green Master Mix, 1 µL of 10× miScript Universal Primer, 1 µL of 10× miScript Primer Assay, 2 µL RNase free water, and 1 µL cDNA. The thermal‐cycling conditions were a PCR initial activation step at 95°C for 15 minutes, followed by 40 cycles of 94°C for 15 seconds and 55°C for 30 seconds. To analyze mRNA expression, we used a One Step SYBR PrimeScript RT‐PCR Kit II (Takara). Polymerase chain reaction amplification was carried out in a 10 μL final reaction mixture containing 5 μL of 2× One Step SYBR RT‐PCR Buffer 4, 0.4 μL PrimeScript One Step Enzyme Mix 2, 0.4 μL forward primer (10 μmol/L), 0.4 μL reverse primer (10 μmol/L), and 100 ng total RNA. Reverse transcription was


no effect on their phosphorylation or expression in vitro and in vivo (data not shown). If growth inhibition by targeting miR‐361‐3p is independent of the ERK1/2 pathway in OSCC, combination with cetuximab could be effective.

In summary, miR‐361‐3p is likely to play a significant role in the growth of human OSCC cells and that targeting miR‐361‐3p may be a useful therapeutic approach for patients with OSCC.

CONFLICT OF INTEREST {#cas14359-sec-0019}
====================

The authors have no conflicts of interest to declare.

Supporting information
======================

######

Table S1

######

Click here for additional data file.

######

Table S2

######

Click here for additional data file.

This work was supported by JSPS KAKENHI Grant Number 24390457.
in Germany and Europe and €40 in the rest of the world will be charged.

A booking is only possible four business days before the desired flight date. The miles required will be automatically debited from your mileage account following the reservation.

Small children (under 2 years of age) travel with you free of charge if they do not need a separate seat.

3.3. Special rules for status membersAn upgrade in the next highest fare class is not possible for Companion Awards. Even if you book your upgrade award at the last minute in a period of seven to four days before departure, as a Senator or HON Circle member, no service fee will be charged for sending your upgrade award to you by express mail.


Assert that the cleanup function was called and iterated the WeakCell.
let timeout_func = function() {
  assertEquals(1, cleanup_call_count);
  assertEquals(1, cleanup_holdings_count);
}

setTimeout(timeout_func, 0);
                                     std::string& basePath,
                                            std::string& videoFileName);

    /** \brief Retrieves FileItems that could contain associated files of a given video.
    *   \param[in]  videoPath The full path of the video file.
    *   \param[in]  item_exts A | separated


village consists of 6 housing units with a total of 40 apartments. The apartments (2 to 4½ rooms) each accommodate from 4 to 8 people.

Each of the 13 Reka holiday villages in Switzerland has an indoor or outdoor swimming pool, an on-site host, the Rekalino family programme with childcare and a free loan service for baby items.

Accommodation

Public Transport

Touristic amenities

*All prices are in Swiss Francs and correct at time of print. Prices shown in currencies other than Swiss Francs are guideline prices only and subject to exchange rate variations. All programmes and prices are subject to change without prior notice.starts here -->

OfficeMax Crescenzo Microfiber Executive Chair

				Item #:  21919024

				Ships In:  1 to 3 days

				Price:
						 $159. 99

										$ 119.99 EA

Quantity
					<input type="text" name="21919024" title="Quantity" maxlength="3" class="cart-quantity textOMX addQuant" value="1" />

Item Total $119.99

Item Total:

$119.99

Sub Total:

$119.99

													 Shipping:


0;
    camR.y = 0;
    camF = camF.normalized;
    camR = camR.normalized;

    transform.position += (camF * vec.y + camR * vec.x) * Time.deltaTime * MoveSpeed;

    Vector3 deltaPosition = transform.position - prevPosition;

    if (deltaPosition != Vector3.zero)
    {
        transform.forward = deltaPosition;
    }

    prevPosition = transform.position;
}

A:

"camera is child object of my character"

Yup, there's your problem. Let's walk through what happens:

We use the camera's forward to compute a new position for the character
We use the new position to compute a new forward direction for the character
The camera is a child of the character, so rotating the character changes the camera's1. Field of the Invention
The present invention relates to a method of estimating wire length. More specifically, the present invention relates to a method of estimating wire length allowing highly precise estimation.
2. Description of the Background Art
In a semiconductor integrated circuit, wiring length becomes larger in inappropriately designed circuitry. In other words it takes more time for a signal to pass through the wiring, possibly resulting in a problem that the circuit operation cannot satisfy the required specification.
In view of the foregoing, in designing semiconductor integrated circuits, signal delay time is calculated based on the wiring length, when design of macro cell placement and wirings between macro cells (hereinafter referred to as "placement and wiring") is completed. Based on the calculated delay time, placement and wiring are modified


"Oh, I know someone." " Great!" "Anne, you're gonna come with me to Larry's house, then, because the second thing we need to do is test his alibi." "And Dwayne, how do you feel about taking care of Larry's first wife?" "I feel like Larry beat me to it." "I want you to retrieve Adelaide Henderson's body from Virginia to get a proper autopsy." "We are going to prove that she wasn't murdered." " I'm on it." " Great." "So, Anne, how do you know this forensic pathologist?" "He's in my OCD group." "You also have OCD?" "No." "Oh, my God, can you imagine?" "I volunteer there." "There's some people out there with issues." "Josh, Larry, this is Thom Hinkle." "He's the best DNA guy in town." "Oh,it into iceberg." "And snake walk." "Right on home." "Hold on!" "I can't hear Anne screaming." "Your story checks out." "Finally, some good news." "Anne." "Anne?" "Anne?" "Oh, my gosh, Anne!" "Oh." "Oh, no, not another one." "_" "So, slight hiccup." "Seems here in Virginia, they use footstones instead of headstones, so we unearthed the wrong body." "No biggie." "Luckily, I found a local to help us right the situation." "All he wanted was 20 boxes of antihistamine." "Now..." "Oh." "[Clears throat]" "Slighter hiccup..." "Josh:" "The prosecution has requested a meeting." "Turns out they don't want this to go to trial, either." "So, now, they'll offer some plea." "You know, it's all part of the dance." "And lucky for me, I got seven years of tap." "Actually, can


you just edit that last part out?" "Death by electrocution." "[Chuckles] Death by electrocution." "That is your offer?" "It's the most compassionate means of execution." "What's the least compassionate?" "Well, technically, death by bear is still in the books in this county." "Where the hell am I?" "A long way from home, counselor." "Why are you so desperate to kill Larry?" "It's nothing personal." "I'm running for D.A., and as a woman," "I need to be twice as qualified as any man, which means I need a death-penalty conviction." "I had my chance when I was younger, but let's just say I was not as refined as I am today." "You heard the defendant threaten to hand the victim's head to him?" "I don't recall." "You don't recall?"Ampullary carcinoma associated with protein-losing gastropathy due to diffuse varioliform gastritis.
Protein-losing gastropathy due to diffuse varioliform gastritis is a rare condition, and its occurrence accompanying ampullary carcinoma is particularly rare. We report here a case of ampullary carcinoma accompanied with protein-losing gastroenteropathy due to diffuse varioliform gastritis. A 39-year-old Japanese woman was admitted to our hospital because of general fatigue and generalized edema. Her total protein level was 3.1g/dL, with an albumin level of 1.4g/dL, and hemoglobin level of 6.9g/dL. Upper gastrointestinal endoscopic examination showed diffuse varioliform gastritis and carcinoma of the papilla of Vater. A diagnosis of protein-losing gastropathy was made based on the results of scintigraphy using technetium 99m-labeled human albumin. Continuous bleeding from ampullary carcinoma caused anemia and deteriorated hypoproteinemia. Pancreaticoduodenectomy was performed for ampullary


carcinoma prior to Helicobacter pylori eradication. The tumor was a papillary adenocarcinoma, which had invaded the lamina muscularis propria over the sphincter of Oddi; the resected stomach revealed typical hyperplastic lymphocytic gastritis. H. pylori were detected on microscopic analysis. Scintigraphy after surgery showed no accumulation of the tracer in the bowel. Anemia, hypoalbuminemia and diffuse varioliform gastritis are improved 6 months after surgery and H. pylori eradication, and the patient is currently free from disease.Until 1965 his territory included LaSalle County and during such period of 16 years he rose to the position of district manager. In 1965 he was transferred to Cincinnati, Ohio and then to Milwaukee, Wisconsin. In June and July of 1967 John Kendrick withdrew his profit sharing funds and resigned. John Kendrick and Marguerite, the appellant, then moved to LaSalle, Illinois and according to plaintiff set up an exterminating business of their own.
A provision of the employment contract between Arwell and John Kendrick provided in substance that Kendrick would not compete directly or indirectly with the business of plaintiff within an area of 20 miles from *633 any place where the covenantor had worked for plaintiff for a period of two years after termination of employment. Admittedly LaSalle,


the trial court that the covenant was enforcible against defendant, Marguerite Kendrick, the gist of the action being that if such covenant was enforcible against John Kendrick, as the court found it was, then equitable considerations authorized and required injunctive relief against Marguerite Kendrick in order to make the injunction against John Kendrick effective. We hold that the trial court acted properly. Marguerite Kendrick was in fact and law a stranger to the employment contract. However she was not a stranger to the transactions and events from which it may be inferred she knowingly participated and aided in the evasion or violation of the restrictive covenant. We know of no rule of law which holds that appellant merely because she was not a party to the employment agreementQ:

An Incredible Theory

I am still relatively new to the Puzzling section of Stack Exchange; but with a single puzzle under my belt, I'm back with another one. It took me some time to put this one together and it has been heavily influenced by a research project. I have broken this puzzle into three parts; I tried to tie them together as much as possible. Your goal is to solve the puzzle with as few parts as possible, keeping in mind that the answer is two words.
Part One:

My prefix is an argument.
My suffix is an emmet.
My infix is an acronym.
I am a synonym.

Part Two:

 Your first thought is an animal, your second is a sin; what you truly desire is an antonym.

Part Two's Hint:

 In reality the word


    “The third factor I am required to look at is the potential impact on the defendant’s
decision to testify. I will consider that factor. I will give it the weight it deserves.
       “And the fourth factor is the issue of multiple priors and how much impeachment
represents proper impeachment versus how much impeachment is too much, whatever
that might be. And again, I would look at that case of Muldrow to determine whether or
not impeachment is appropriate.” The trial court ruled that if defendant testified, he
could be impeached with all five of the prior offenses involving moral turpitude,
including the 1980 misdemeanor convictions for battery upon a police officer and
domestic violence.

           could describe the total number of
wounds and offer a brief description, but added they should not get into too much detail.
Before calling the rebuttal witnesses, the prosecutor again sought to clarify the scope of
the trial court’s ruling, noting that in the prior assault half of the victim’s ear was almost
severed. The trial court said she could ask about the location and number of stab wounds
but could not elicit testimony about the severity of the injuries sustained by the victim.

                                              11


facts regarding defendant’s prior assault with a deadly weapon
conviction and his aggressive conduct upon being arrested were highly probative to rebut
defendant’s claim that Donald was the aggressor in the fight. Nevertheless, the trial court
limited the details of the prior conviction, barring details of the wounds and their severity.
That limitation reduced the inflammatory effect and limited the amount of trial time
consumed by presentation of the evidence. The trial court weighed the probative value of
the evidence and “understood and fulfilled its responsibilities under Evidence Code
section 352.” (People v. Williams (1997) 16 Cal.4th 153, 213.) The evidence was not
unduly prejudicial and the trial court did not abuse its discretion.
                      2020 coronavirus outbreak in the Philippines

This article documents the impacts of the 2019–20 coronavirus outbreak in the Philippines, and may not include all the contemporary major responses and measures.

As of March 1, there are three confirmed cases in the Philippines. Out of the three cases, one has died. The first case of coronavirus disease 2019 (COVID-19) in the Philippines was confirmed on January 30, that of a 38-year-old Chinese woman who was confined at the San Lazaro Hospital in Manila. The second case was confirmed on February 2, that of a 44-year-old Chinese man who died a day earlier which is also the first confirmed death from the disease outside mainland China.

The Research Institute for Tropical Medicine in Muntinlupa is the medical facility where suspected cases are being


to be repetitive.

* * *
"Q. Were you able to form an opinion whether Mr. Griffes is a sexually delinquent person within the meaning of that statute you have read to us?
"A. It is my belief that he is.

* * *
"Q. Would it be correct to say that these acts are less voluntary on his part than the normal acts of any kind that all of us engage in?
"A. Yes, sir. This is not a voluntary thing. I would acknowledge that Mr. Griffes doesn't plan to do this. This happens to him in a state of accumulating sexual and attention-seeking tension.
"Q. Now, this thing which is defined in the statute as sexual delinquency or sexually delinquent person and which you find that he fits in under the compulsive orfor their illness or punished for it, is a question which touches the very heart of judicial consciousness of a civilized system of jurisprudence. If, perchance, the parties or counsel fail to raise the matter our courts cannot close their eyes to so important a matter. Under facts such as indicated in the present case, the trial judge should have initiated criminal sexual psychopath proceedings on his own motion once the defendant was convicted of the offense charged. It is essential to the dignity of the jurisprudence of this State that we do not punish mental disorder.
Since the proceedings under the criminal sexual psychopath statute may be initiated at any time prior to sentencing (CLS 1961, § 780.503 [Stat Ann 1954 Rev § 28.967(3)]) the sentence entered is


Q:

Change color of elements using jQuery

This should be easy but I'm not getting it for some reason. How would I set the CSS color style (e.g. color:green) of all the elements who are of class 'foobar' using jQuery?

A:

You want the color to go green just on mouseover? Can you be more specific?
$('.foobar').mouseover( function() {
  $(this).css( { color: 'green' } );
} );

for actual in vivo use. Non-enzymatic in vivo sensors (e.g. for the detection of the monoamine neurotransmitters dopamine and serotonin, where the analyte is electroactive and can be converted directly at the electrode) are not discussed either \[[@CR4]--[@CR7]\].

In this article, we describe and compare different platform and sensor integration technologies for enzyme-based amperometric in vivo microsensors, discussing work relevant to the current state of the art regarding fabrication and performance. We compare the microsensor approach with other techniques to illustrate where such sensors can be applied, what advantages can be expected and how they complement other methods. We describe the different microfabrication techniques for amperometric in vivo sensors, ranging from simple modified wires and fibres to fully microfabricated systems on silicon, ceramic or polymer substrates. We discuss recent


are present there, it is clear that current amperometric microsensors cannot aim at measuring "synaptic" neurotransmitters, but rather can aim at changes in the extrasynaptic background. Neurotransmitters also have a multitude of functions beyond synaptic neurotransmission \[[@CR9]\]. Furthermore, the glutamate--glutamine cycle is the main energy consumer in the CNS, underlining the relationship between energy and neurotransmitter metabolism \[[@CR12], [@CR13]\].

### Energy metabolites {#Sec5}

Glucose as the primary energy source for cells is normally supplied to most tissues through the bloodstream in relatively high and stable concentrations (4--6 mM), especially the brain (1--3 mM) \[[@CR14]\]. Lactate is the main product of anaerobic metabolism. Besides being a direct indicator for the amount of anaerobic metabolism, it is more than a metabolic waste product. It can act as a neuroprotector and under certain conditions asclosely linked to cancer. Inside tumours a hypoxic environment with a highly anaerobic metabolism is often found \[[@CR19], [@CR20]\]. Any disruption in blood supply and thus oxygen and energy supply (e.g. during surgery, transplantations, sepsis or trauma) will lead to alterations in metabolism. Here another field of activity for microsensors may evolve in the future.

### Enzyme-based amperometric microbiosensors {#Sec6}

Enzyme-based amperometric microbiosensors are typically realized by a set of immobilized membranes on noble metal or carbon-based electrodes (Fig. [1](#Fig1){ref-type="fig"}). In a first-generation biosensor, an enzyme converts the analyte to an intermediary product, which is then oxidized at the electrode \[[@CR1]\]. The resulting current flow is proportional to the analyte concentration. The enzyme proteins have to be immobilized in a matrix or membrane without their functionality being lost. Most commonly, these


allow continuous monitoring. Online methods include use of electrochemical sensors and capillary electrophoresis. Yet, the time delay even for rapid systems is still non-negligible. For electrochemical sensors it ranges from 30 s to a few minutes \[[@CR58], [@CR59]\]. For online capillary electrophoresis with laser fluorescence detection, a temporal resolution of 12 s was reported for glutamate measurement in animals \[[@CR60]\]. A rise time of 60 s was achieved by on-chip electrophoresis with amperometric detection \[[@CR61]\]. Still, the fast dynamics of some neurotransmitters in combination with low concentrations are impossible to measure by microdialysis. Additionally, the measurement of dissolved gases with high diffusivities such as oxygen is very cumbersome with external microdialysis methods because of high out-diffusion during transport.

### Nuclear magnetic resonance {#Sec10}

Especially in the neurosciences, NMR techniques are used not onlyonly the creation of an electrode and contact pad opening through the insulating layer but also the shaping, thinning and release of the entire needle-type structures in the wafer plane (e.g. by deep reactive ion etching). Inconveniences are the need for costly equipment and clean-room facilities for fabrication as well as the brittleness of silicon, which limits the overall device length and robustness.

A silicon process was used by Frey et al. \[[@CR29]\] for the fabrication of glutamate and choline microsensors for the brain of rats (Fig. [2d](#Fig2){ref-type="fig"}). The silicon needles were 30--100 μm thick, 100 μm wide and 6.5 mm long, with the two working electrodes located at the tip, and a Ag/AgCl reference electrode was integrated further behind. The wafer-level processing included the physical vapour deposition of platinum as the electrode


of the material, is much larger for silicon though. Most likely, the thin ceramic substrate limits the reported size of the wafer/substrate (2.5 cm × 2.5 cm \[[@CR88]\]), whereas in silicon, individual chips can be thinned out and standard wafer sizes (e.g. 100 mm diameter at 300 μm thickness) can be used. Dicing by blade of the ceramic substrate allows only limited geometric shapes, and additional, chip-level modifications such as laser cutting are needed for final shaping.

Polymer-based platforms {#Sec17}
-----------------------

The increase of biocompatibility and the ease of use of flexible, polymer-based platforms has is desirable for both in vivo sensors and neurotechnological devices. One of the primary limitations though is that unguided penetration into tissue (e.g. into the brain in a stereotaxic setup) is not possible for very thin probes. Most developments in this fieldhave been for neural implants rather than biosensors. Still, an overview of the technologies applied may be helpful for biosensor development.

Polyimide resin can be spin-coated on silicon wafers and sputtered with platinum as electrode material, which is then insulated by another spin-coated polyimide layer \[[@CR91]\]. Electrode opening and shaping of devices can be achieved by reactive ion etching. Electrodes as small as 10 μm and with a total thickness of less than 20 μm can be achieved. Polyimide in a precursor state can also be laminated on such structures to achieve additional layers \[[@CR92]\]. As a shaft for an optical waveguide, including a microchannel for drug delivery into brain tissue of mice, 10 μm spin-coated polyimide as the substrate was combined with 160-μm-thick SU-8 permanent epoxy resist as the waveguide \[[@CR93]\].


a Ag/AgCl reference electrode were included. Enzyme immobilization was by entrapment in poly(hydroxyethyl methacrylate)-based (PHEMA) hydrogels. Lactate sensors were later added on a modified device \[[@CR21]\]. For glutamate, glucose, lactate and oxygen in vivo sensors for both the brain and subcutaneous applications, we used a polyimide film of thickness between 50 and 100 μm and insulated the platinum electrodes with 5 μm SU-8 epoxy resist on both sides \[[@CR26], [@CR44]\] (Fig. [2c](#Fig2){ref-type="fig"}). Additionally, we introduced a laminated, 38-μm-thick negative dry film resist as another layer. The main feature of this relatively thick material is that it forms an even deeper well around the electrode in comparison with that obtained with other methods \[[@CR23], [@CR102]\], which allows volume control during sensor membrane deposition. By the stacking of membranes, the control of sensitivitywhich are used to expand the linear range of the sensor (Table [3](#Tab3){ref-type="table"}). By variation of the thickness of the enzyme membrane and/or the diffusion-limiting membranes, the sensitivity and linear range can be adjusted over a very wide range without compromising sensor performance parameters other than the response time. Even though glutamate oxidase can be immobilized this way, the concentration of the enzyme cannot be high enough to achieve highly sensitive signals comparable with those obtained with the cross-linking method.

The PHEMA hydrogel stabilizes the enzymes very well, regarding both the natural conformation and thus activity and the confinement/fixation of the enzyme inside the membrane, and therefore leads to long-term stable sensors, particularly for otherwise difficult to immobilize lactate oxidase \[[@CR26], [@CR110]\]. Besides, the PHEMA membrane is mechanically durable


oxidase was also co-immobilized to improve selectivity \[[@CR114]\]. The performance was comparable to that of the first-generation biosensors (Table [3](#Tab3){ref-type="table"}), although response time at more than 20 s was slower \[[@CR40]\], most likely due to diffusion limitation by the dense gel. The hydrogel was applied to carbon fibres by dip-coating. Not dependent on the hydrogen peroxide oxidation at the electrode, the applied potential can be lowered to −150 mV (vs Ag/AgCl) \[[@CR40]\]. The method was also applied for choline and acetylcholine sensors \[[@CR115]\].

### Electrodeposited polymers {#Sec22}

Besides their use as permselective layers to reject interferents, electrodeposited polymers can also be used to immobilize enzymes. These thin polymer/composite membranes in the range of 10 nm can entrap the active enzyme on the electrode and block interfering substances at the same time \[[@CR116]\]. Inengineering perspective as well as the application perspective.

Microtechnology offers a multitude of possibilities to fabricate and integrate microsensor platforms for in vivo application. Particularly in silicon technology, new platforms have emerged, based on recently optimized fabrication pathways. Wafer-scale processing, including reference electrode integration, and even membrane/sensor integration can mean an efficient mass fabrication of ready-to-use devices. The development of new sensing principles, however, is often done on a macroscopic scale. There must be a translation of these principles to usable platforms so that sensors do not share the common fate of remaining "laboratory curiosities" \[[@CR135]\]. The amperometric in vivo biosensors discussed here all employ fairly traditional sensing principles. Thousands of new enzyme immobilization concepts and electrode materials are developed that have surprisingly little impact on sensor performance. Many


An appropriate enzyme (or enzyme system) must exist, and it must be immobilized in a stable way. There is often a trade-off between sensitivity and quick response, and long-term stability. If the enzymatic reaction requires oxygen, a dependency is to be expected if oxygen supply is restricted. Diffusion occurs to and through the sensor membrane, so all signals are diffusion limited, with diffusion coefficients constant by nature.

We did a comprehensive performance comparison for enzyme-based amperometric in vivo microsensors (Table [3](#Tab3){ref-type="table"}). The key performance data, such as sensitivity, linear range and detection limit, fall in a relatively small range, even across platforms, electrode materials and immobilization methods. Sensitivity of around 2 nA mm^−2^ μM^−1^ and a detection limit below 1 μM are feasible. The "selective membrane/platinum/hydrogen peroxide/oxidase enzyme" system seems to bedegradation are desirable. For quantitative long-term measurements, in vivo calibration schemes are inevitable. Flexibility of the devices is an underrepresented aspect regarding structural biocompatibility. The process of insertion is a challenge for flexible devices.

Potentiostats and instrumentation are essential for amperometric sensor operation. Advanced electrochemical protocols require more sophisticated instruments, which cannot be self-made easily and which are different from those in neural recording. Commercially available instruments are often large and expensive. Measurements on freely moving animals require headstages, which are often available only for proprietary sensors. The combination of disposable microsensors and miniaturized electronics leaves room for improvement. With regard to portable or wireless sensors as well as multichannel sensors, technological developments in this direction are necessary.

Conflict of interest {#FPar1}
====================

The authors declare that they have no conflict of


Q:

In C, How can I evaluate a local variable thats in one function, in a different function?

Ok so I'm creating this text adventure game. To be all unique and different, I decided to add an influence system where your responses to other characters can affect their responses to you as well their combat effectiveness.
I have a fair amount of experience in C++ and I just started my second semester-long course in C. So far, I have tried using global variables, structs, static variables, and using functions inside of other functions. I also tried using pointers but I kept getting errors every time so I stopped. This is a snippet of code that tries to use the influence system(Sorry for the stars, wouldn't want to give away any storyGraincorp to sell off Emerald depot

GRAINCORP will sell off its Emerald depot and relocate out of town as part of a nationwide $200 million restructure of its operations.

The move could pave the way for a future residential development on the 10ha site, Central Highlands Mayor Peter Maguire said.

"In the council's new strategic framework for its new planning scheme, that side of town is one of the areas identified for future urban expansion," Cr Maguire said.

"With the depot's proximity and more and more residences coming to that side of town, I respect Graincorp's decision to move on.

"I am sure it is valuable residential real estate for the future."

Graincorp spokesman Angus Trigg said negotiations were already under way for the new site as part of Project Regeneration.

It is hoped to


to understand parts of speech and get an idea on why they are important.
Thank you

A:

For English, you probably forgot articles.
Other languages usually have different sets of parts of speech (or lexical classes), and the Wikipedia article provides with a decent list of those. For a more detailed analysis, check David Beck's "The Typology of Parts of Speech Systems", chapter 4 "Types of lexical inventory".

As one of interesting examples, Thai has noun classifiers (measure words). They are used to "compensate" the absence of plural number and also to improve the "rhythm" of the language (a pretty subjective thing which is often forgotten by language learners but is important to native speakers).
There are classifiers for types of nouns (animals, people), or based on shape of the objects (round, square, cigar-shaped \label{eq:H0}
  \end{aligned}$$ where $\epsilon_{\bm{k}} = 2 D J S ( 1 - \gamma_{\bm{k}} )$, with $$\begin{aligned}
    \gamma_{\bm{k}}
    &=
    D^{-1} \sum_{\mu = 1}^D \cos ( {\bm{k}} \cdot
    {\bm{a}}_\mu )\,.\label{eq:gamma_k}
  \end{aligned}$$ For simplicity, we impose periodic boundary conditions on a hypercubic lattice with primitive lattice vectors $ {\bm{a}}_{\mu}$ and lattice spacing $a = | {\bm{a}}_{\mu} |$. The momentum sum is over the first Brillouin zone and $\hat{b}_{\bm{k}}$ is the lattice Fourier transform of $\hat{b}_i$. The corresponding free energy is $$\begin{aligned}
    F_{0} (  h ) =
    - D J N S^2 - h N S  + T \sum_{\bm{k}} \ln
    \left[ 1 - e^{


High-fat diet blocks the inhibition of skin carcinogenesis and reductions in protein kinase C by moderate energy restriction.
The aim of this investigation was to determine the impact of dietary energy restriction (ER) with control (C) and high-fat (HF) diets on two-stage skin carcinogenesis and on the expression of specific isoforms of protein kinase C (PKC). Skin carcinogenesis was initiated on SENCAR mice with 10 nmol of 7,12-dimethylbenz[a]anthracene (DMBA) in 0.2 mL of acetone and then promoted with twice weekly treatments of 3.2 nmol of 12-O-tetradecanoylphorbol-13-acetate (TPA) in 0.2 mL of acetone for 18 wk. The experimental diets fed during TPA treatment and for 10 wk after the last TPA treatment were formulated with C (10% calories from fat) and HF (42% calories from fat) levels for freely fedThe Hyundai Veloster has always been one of the most peculiar-looking vehicles on the market. For the 2019 model year, Hyundai overhauled the design, integrating it more into the rest of its lineup while keeping the model’s personality intact. But the question is: Did Hyundai go overboard in either one of these two areas?

The Veloster’s new face falls in line with the look of other new Hyundais. It gets the brand’s signature “cascading” grille that features sharper angles than the grille on the previous Veloster. New wedge-shaped headlights contribute to a bolder, less curvy look. The 2019 Hyundai Veloster also receives new lines on the hood that extend far out unlike the lines on the old model that are centered near the windshield.

Hyundai kept the Veloster’s unusual three-door


under 1,900 pounds, and the horsepower and torque of the two motors totals 429 hp and 563 lb-ft. I plugged all these figures into another program, along with a roughly estimated drag coefficient and frontal area (aero isn’t too important below 60 mph), and the computer indicated that this acceleration rate would demand 435 hp. Close enough to assure us Aspark isn’t sand-bagging on the power number.

Less clear at this point is the driveline setup. The brochure claims a 174-mph top speed with a maximum motor speed of 4,000 rpm. Given the claimed final-drive ratio of 4.44:1 and the tire sizes (275/30R19 front, 335/30R20 rear), a two- or three-speed gearbox at least will be needed to hit that speed, though a 1:1 first gear would reach 72 mph.

Whothe corner first, so you approach it gingerly. You quickly find, though, it’s just a little rotation like the man said. You can carry more speed. You don’t need to brake as much next time, or as early. The car has so much more to give, and you find a little bit more each time by.

It’s aggravating to realize how much time you’ve left on the table, and the need to find it is all consuming, and so the GT3 goads you on. With every lap, you push your brake point a little later, carry a little more speed into the corner, and get back on the power a little sooner, feeling out the point when a little rotation becomes a big rotation and a bigger repair bill.


have a little tear drop trailer that I drag behind and it’s a little much for that FJ, but that’s one of the reasons I’m excited about the Defender 90—is the towing capacity.”

A 2007 Toyota FJ Cruiser is shown here

As someone who is into aesthetics, Nelson has always admired Toyota FJ Cruisers. “They just have such a different look to them and … I always love the idea of being able to go deep into the woods and do some four-wheel off road driving. I say that but it’s a lot like my fishing, I talk about fishing a lot but I rarely fish. I rarely go off road, but just knowing that it would be a possibility is comforting to me,” he says, with a laugh.

“Just inwere bald because he couldn’t afford new ones. “We hit one of those Kansas rain storms and all of a sudden we were hydroplaning … and we just spun around like a wing nut on the elevated highway and came to a stop on the divider. We didn’t damage anything. Somehow we survived and off we went and we were like, ‘Wow.’”

Animal Planet’s “Treehouse Masters”

Although his Defender is the most recent childhood dream fulfilled, Nelson’s life in itself has been living out a dream. He realized early on that working with wood, building things, and being a carpenter was his raison d’etre.

“I would get very excited and passionate about building a little boat model, or even a deck on the back of the house in the suburbs of


have a good idea exactly what the new Supra will look like.

Other than the generally legitimate look of the magazine, this (admittedly poor) image shows a race-prepped version of the Supra. If you compare it with the official teaser shown below that Toyota released a few days ago, the rounded rear fenders, double-bubble roof, and massive wing all match up. This is also the version we’re most confident will be at the Geneva Motor Show next month, but hopefully, Toyota reveals the road car, as well.

Without its GT racing-style livery and bodywork, we see a lot of the 2014 Toyota FT-1 concept in the new Supra. The styling has been toned down a little, but overall, it’s a fairly handsome sports car. We especially like the look ofwill buy a very nice house. But on the other hand, can a house accelerate from 0-124 mph in 9.6 seconds? Does a house have a retractable roof? Even if a house did have a retractable roof, could it hit 196 mph with its top down? Because the McLaren 570S Spider sure can.

Even better, imagine the look on your significant other’s face when they realize the small box you gave them contains a car key instead of a necklace. Not just any car key, either. The key to a bright red convertible supercar. Surely true love is worth 30 years of car payments, right?

Before its debut in Geneva next month, the four-door Mercedes-AMG GT is undergoing aerodynamic tests in the wind tunnel. And we now have a better


kind of checklist of: Is my architecture timeless? Is it responsive in such a way that it’s likely to be meaningful on a long-term basis?”

Dimensions of learning

“In the early parts of my career, I was quite obsessed with geometry and with the notion of creating three-dimensional spatial components, as building blocks for construction. Habitat (Safdie refers to his seminal Habitat 67 housing complex in Montreal he designed in 1967. AN) is an example where boxes form houses, but then I tried to carry that thought process to other typologies. At some point, I realized that different typologies require different systems and that there’s a wide variety of building systems, all of which could lead to a wider variety of expressions. So this was a big lesson. A lessonan architect fifty years and you already had three buildings demolished, and you see the transformation that’s taking place, very little or none of it is forever. I’ve seen architecture go from profound concerns for society as a whole to a period of interest in tantalizing society by the possibilities of architecture. I’ve seen the public awed by certain buildings because of their notoriety for a while, but there’s a quality of being impressed and there’s a quality of affection and loving something.

I go to Habitat today, it’s fifty years old and, not just to my mind but to almost every observer, it’s as fresh as ever. It’s as relevant as ever. After years of being semi-ignored, all of a sudden the ideas of Habitat are all over


should be."

Now, my son is a toddler, and he just likes trains and turtles. But even if the political underpinnings of the Yertle and Thomas books are way over his head -- not hard, as he's only about three feet tall -- they still impart moral lessons. One reinforces class divisions and imperiousness while the other promotes equality and compassion.

But apparently equality and compassion aren't appropriate for B.C. classrooms.as well. Unfortunately, both aforementioned fixed combinations and NSAIDs do not prevent the temporary symptoms of ocular surface disease (OSD) that almost all cataract patients experience. Therefore, artificial tears are gaining popularity as an additional integral component of the postoperative regime in order to alleviate OSD-related symptomatology.

Among the popular artificial tears medications are the: a) polyethylene glycol 400/propylene glycol/hydroxypropyl-guar (Systane Ultra, Alcon, Greece) consisting of Polyethylene Glycol 400 0.4% and Propylene Glycol 0.3% and b) 0.1% sodium hyaluronate provided in the continuous monodose system COMOD® (Hylocomod, Farmex, Greece). COMOD device is an integral airless application system, which enables delivery of multiple sterile doses of a liquid medicinal product. Both medications are considered to be highly effective in dry-eye-disease (DED) and prospectively in moderate and severe OSD. However, both


"Anybody could run this app and then go hang out near some people they want to cause trouble for."

If the report of symptoms goes through a central database first, however, the app can take the user through a Q&A to find out if the cough is new and persistent, and if other symptoms have emerged, before assessing whether an alert is worth broadcasting.

Therefore, it "absolutely makes sense", according to Anderson, that NHSX decided to drop Apple and Google's API, even if the decision worries some privacy advocates. "We need to avoid getting into a fight between different camps, because it is a sheer distraction from the real task at hand, which is saving lives," he said. "A small amount of privacy exposure is entirely acceptable in the contextdevelopments present. This exchange enables a mixed approach to digital leadership and is vital and unique to the project.

Among some of the feedback we have received after the workshop, was that the tools provided and the reflection space, has helped leaders to not only receive information, but also enabled them to take actions.

But these insights and knowledge exchanges are not available only to workshop participants. Leaders can also request membership to our I4L Community and join the conversation there.

Follow-up Interviews: Workshop Impacts in Practice

One of the leaders stated that after the workshop he became more conscious about how he uses digital communication tools.

When I’m not using (Digital Communication Tools) for a meeting, I’m using them to engage. Knowing the person in the other end, instead of just having


the “kindness factor”. After two months, we interviewed the leader to learn more about it:

Q: One of the things you wanted to work on was something you called the “kindness factor”, and you also explained that due to your technical background you had a tendency to give too many technical details, and that you’d like to work on that. I wanted to hear how did that go?

A: Well, let’s put it this way, it’s very hard to do that, but I think I moved away from the very deep technical interaction into a more strategic approach in the work that I do. You can’t go to the CEO and tell him about the technical details. He will just look at you and go blank… And looking at itinsight into how digital communication practices that convey care and kindness, and are attuned to the person we are communicating to, has a positive impact on the interaction.

Are you interested in applying to participate in our next workshop on Transformational Leadership and Disruptive Innovation, then learn more and apply here.

To stay updated on our research insights and news about our next workshops, remember to subscribe to our newsletter and follow us on Twitter @i4l_dk.

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Transformational Leadership and Disruptive Innovation Date: April 9th 2018 Place: IT University of Copenhagen Duration: 8.30 – 16.00 I4L (“Innovation for Leadership”) calls for applicants to participate in the workshop: “Transformational Leadership and Disruptive Innovation”. The day-long workshop will take place on Monday April 9th, at the IT University


suggesting that different mechanisms such as *PIK3CA* mutations involve activation of AKT[@b10]. The lipid phosphatase activity and protein stability of PTEN can be regulated through multiple mechanisms, including acetylation, phosphorylation and ubiquitination[@b11]. For instance, E3 ubiquitin ligases, NEDD4-1 and WWP2, have been reported to decrease PTEN stability by catalysing PTEN polyubiquitination, and the PTEN degradation predisposes to tumourigenesis[@b12][@b13][@b14]. In addition, PTEN is subject to phosphorylation at the C-terminal serine--threonine cluster (Ser370, Ser380, Thr382, Thr383 and Ser385) that affects its phosphatase activity, subcellular localization and protein stability[@b15][@b16][@b17]. It has been proposed that phosphorylation of the C-terminal sites keeps PTEN in an inactive form in the cytoplasm; however, the dephosphorylation induces translocation of PTEN to the plasma membrane where the active PTEN antagonizes PI3K-AKT signalling[@b18]. PTEN inactivation through increased phosphorylationthe ATLL samples except the SO4 cell line ([Fig. 2c](#f2){ref-type="fig"}), we performed a mutation analysis of the *PTEN* and *PIK3CA* genes. No mutations were detected within the coding and adjacent intronic regions of *PTEN* or in the hotspot mutation regions of *PIK3CA* in the ATLL samples ([Supplementary Table 3](#S1){ref-type="supplementary-material"}). Therefore, we considered the possibility that post-translational modifications of PTEN influence PTEN phosphatase activity in ATLL cells. The phosphorylation of PTEN at the serine--threonine cluster (Ser370, Ser380, Thr382, Thr383 and Ser385) in the C-terminal tail suppresses its phosphatase activity[@b17]; therefore, we evaluated PTEN phosphorylation and demonstrated that the cluster sites were highly phosphorylated in the primary ATLL cells and ATLL cell lines, whereas the non-phosphorylated form was lower in abundance compared with controls ([Fig. 2a,b](#f2){ref-type="fig"}; [Supplementary Fig. 3](#S1){ref-type="supplementary-material"}). Notably,


factor-1 target gene[@b40][@b41]. It could be speculated that *NDRG2* expression is induced by hypoxia-ischemia in the brain to regulate PTEN phosphorylation, leading to the suppression of PI3K-AKT activation and induction of apoptosis. Interestingly, several kinases, including SGK1 (serum- and glucocorticoid-induced kinase 1), PKCθ (protein kinase C-θ) and AKT, have been found to phosphorylate NDRG2 (refs [@b42], [@b43]). These phosphorylation events are likely to affect the cellular function of NDRG2. It is intriguing to speculate that NDRG2 might be a downstream target of the PI3K-AKT signalling pathway that may participate in a negative feedback loop in PI3K-AKT signalling. PP2A exists predominantly as a heterotrimer composed of catalytic C, structural A and one member of four families of regulatory B subunits. The activity, substrate specificity and subcellular localization of the(Miltenyi Biotec) according to the manufacturer\'s instructions.

Antibodies and reagents
-----------------------

A synthetic peptide (17-PGQTPEAAKTHSVET-31) of human NDRG2 conjugated to keyhole limpet haemocyanin was used for immunization to generate a rabbit polyclonal antibody against NDRG2 (ref. [@b55]). Mouse monoclonal (M2) and rabbit polyclonal (F7425) antibodies against FLAG, and mouse monoclonal antibody (AC-15) against β-actin were purchased from Sigma-Aldrich. Rabbit monoclonal antibodies against PTEN (138G6), phospho-AKT (Ser473) (D9E), phospho-AKT (Thr308) (244F9), phospho-GSK3β (Ser9) (D85E12), GSK3β (27C10), and PP2A C Subunit (PP2Ac) (52F8), rabbit polyclonal antibodies against phospho-PTEN (Ser380/Thr382/383) (9554), non-phospho-PTEN (Ser380/Thr382/383) (9569), AKT (9272), FOXO1/4 (9462) and cleaved caspase-3 (9661), and mouse monoclonal antibodies against PTEN (26H9) and Myc-tag (9B11) were obtained from Cell Signaling Technology. Rabbit polyclonal antibodies against phospho-PTEN (Ser370) (07-889) and PI3K p85 (06-195) were obtained from Upstate/Millipore, rabbit


(Takara) following the manufacturer\'s instructions. The resulting cDNA was used for real-time RT--PCR using a SYBR Green PCR Master Mix kit (Applied Biosystems). PCR was performed in a 25-μl volume containing 1 μl cDNA, 300 μM of each primer and 12.5 μl of 2 × PCR master mix under the following conditions: 95 °C for 10 min followed by 40 cycles of 95 °C for 15 s and 60 °C for 1 min. For NDRG2 primers, the cycling conditions were 95 °C for 10 min, 40 cycles of 95 °C for 15 s, 60 °C for 20 s and 72 °C for 40 s. The primers used were as follows: for *PTEN*, PTEN-F (5′-CAGCCATCATCAAAGAGATCG-3′) and PTEN-R (5′-TTGTTCCTGTATACGCCTTCAA-3′); for *NDRG2*, NDRG2-F (5′-CTGGAACAGCTACAACAACC-3′), and NDRG2-R (5′-TCAACAGGAGACCTCCATGG-3′); and for β-actin, ACTB-F (5′-GACAGGATGCAGAAGGAGATTACT-3′), and ACTB-R (5′-TGATCCACATCTGCTGGAAGGT-3′). The data were normalized to the amount of β-actin mRNA, and the valuesare represented as the mean±s.d. of 2^−ΔΔCt^ in a duplicate assay.

Western blot analysis
---------------------

Cell lysate samples were prepared in NP-40 lysis buffer (50 mM Tris--HCl, pH 8.0, 150 mM NaCl, 5 mM EDTA, 1% NP-40) supplemented with protease inhibitor cocktail (Sigma-Aldrich) and phosphatase inhibitors (PhosStop, Roche) or by direct lysis in boiling Laemmli SDS sample buffer (62.5 mM Tris--HCl, pH 6.8, 2% SDS, 25% glycerol, 5% β-mercaptoethanol and 0.01% bromophenol blue). Protein samples were electrophoresed on 10% SDS-polyacrylamide gel and transferred to polyvinylidine difluoride membranes (Immobilon-P, Millipore). The membranes were blocked in Tris-buffered saline (TBS)-Tween (0.1%) with either 5% bovine serum albumin (BSA) or 5% nonfat dried milk and were then incubated with each primary antibody diluted in TBS containing 0.1% Tween 20 supplemented with either 5% nonfat dried milk or 5% BSA


phosphate-buffered saline (PBS) and incubated with 2 mM DTBP in PBS at room temperature for 30 min. After washing twice with PBS, cells were lysed in TNT buffer (10 mM Tris--HCl, pH 7.5, 150 mM NaCl and 0.5% Triton X-100) supplemented with protease inhibitor cocktail (Sigma-Aldrich) and phosphatase inhibitors (Halt phosphatase inhibitor cocktail, Thermo Scientific), and the lysates were immunoprecipitated with control rabbit immunoglobulin G, antibody against PTEN (138G6, 1:500), or FLAG M2 affinity gel, followed by immunoblotting with antibodies against FLAG (F7425), PP2Ac, PTEN (138G6) or phospho-PTEN (Ser380/Thr382/Thr383).

Immunofluorescence staining
---------------------------

Cells were fixed with 4% paraformaldehyde for 10 min at room temperature, washed with TBS 0.1 M glycine, treated with 0.2% Triton X-100 and rewashed with TBS 0.1 M glycine. After blocking with 1% BSA in TBS, cells were incubated with primary antibodies againstsubjected to western blotting. (**e**) The proliferation rates of WT, *NDRG2*^+/−^, and *NDRG2*^−/−^ embryonic fibroblasts. The mean±s.d. is shown; \**P*\<0.05 (Student's *t-*test). The data are representative of three experiments. (**f**) Kaplan--Meier survival curves of WT (*n*=15), *NDRG2*^+/−^ (*n*=31) and *NDRG2*^−/−^ (*n*=12) mice up to 24 months of age. The difference in survival was statistically significant between WT and *NDRG2*^+/−^ or *NDRG2*^−/−^ mice (\**P*\<0.05, log-rank test). (**g**) A hepatocellular carcinoma section from an *NDRG2*^+/−^ mouse liver was subjected to H&E staining. The results indicated the presence of a large sheet of hepatic cords composed of several hepatocytes of variable nuclei and cell sizes; the nuclei of the carcinoma cells were hyperchromatic with prominent nucleoli (scale bar, 22 μm). (**h**) A bronchoalveolar carcinoma section from an *NDRG2*^+/−^ mouse lung was subjected


Q:

Liferay No Folder Update Mail Notification on Document upload

I'm subscribed to a document folder.
I've setup mail configuration and I receive update emails after some document is edited (e.g. Description change).
When someone add folders or media documents inside my subscribed folder, I don't get any email.
Is this supposed to work as it is?
Any suggestions?
(Liferay 6.2.0)

A:

The solution is here: https://github.com/brianchandotcom/liferay-portal/pull/15426/files
You have to implement an ext that override liferay-portal / portal-impl / src / com / liferay / portlet / documentlibrary / service / impl / DLAppHelperLocalServiceImpl.java
You start from old class and add only necessaries changes (Not the entire file!)
It's the easiest way.

NUR-SULTAN (TCA) — In a telephone conversation at the initiative of the British side, the Minister of Foreign Affairs of Kazakhstan Beibut Atamkulov and the Minister of State for Europe and the Americas of the Foreign and Commonwealth Office of the United Kingdom (FCO) Christopher Pincher confirmed mutual interest in further strengthening and expanding the strategic partnership between the two states and discussed plans to organize future high-level bilateral visits and events, the Kazakh Foreign Ministry reported on August 8.

The Kazakh Foreign Minister congratulated the British politician on his recent appointment and highlighted his interest in further developing constructive relations with the new leadership of the FCO and the UK Government.

Meetings of the Strategic Dialogue at the level of the foreign ministers and the Intergovernmental Commission for Trade,


upon our decision in Polson, we find that the Act at issue also precludes recovery under a state-law claim for retaliatory discharge.  In this Court's view, the remedies under the Act like the remedies under KAAD in Polson are indeed adequate to compensate an aggrieved employee.  These remedies include (1) reinstatement to former position with compensation (including back pay);  (2) compensatory damages;  and (3) all costs and expenses (including attorneys' and expert witness fees).  42 U.S.C. Sec. 5851(b)(2)(B).  Additionally, if a person fails to comply with an order issued by the Secretary of Labor, a district court may grant all appropriate relief including injunctive relief, compensatory, and exemplary damages.  42 U.S.C. Sec. 5851(d).

7
In this case, Masters is seeking compensatory damages, exemplarycompromise but we will do so in love and kindness,” adding bible scripture to justify their handling of the awkward situation.

The family posted the verse Luke 6:27, which reads, “But I say to you who hear, love your enemies, do good to those who hate you, 28 bless those who curse you, pray for those who mistreat you.”

They called the passage “a sweet scripture that we hold dear,” and “a reminder of how we must always carry ourselves,” adding that they’re committed to “treating others well even if we don’t agree with them, and … will do so without hateful words.”

So far, more than five thousand people have signed Wissick’s petition.

Do you think the Duggars’ show, 19 Kids & Counting, should be cancelled because of their beliefs? Let


indictment charging him with concealing on and after May 7, 1953 an adding machine belonging to the corporation.

21
The indictment charged both appellants with seven separate offenses and the appellant Gilbert Edwards with an additional separate offense; all eight counts charged the fraudulent concealment of property belonging to the estate of the bankrupt corporation, to-wit, six specified sums of money, one specified a cash register, and one specified an adding machine. We hold that in law and in fact the eight charges constitute but one offense against each appellant. The eight counts were drawn under the first paragraph of Section 152, which we have quoted above, which denounces the fraudulent concealment of "any property belonging to the estate of a bankrupt". It is clear to us from the plaina headlamp assembly having a plastic lens but there is no suggestion of a dirt build-up deterioration of the lens affecting the transmission of light.
While suitable for their intended purpose, the aforesaid prior art arrangements do not disclose the use of headlamp cleaning systems especially adapted for use with light sources such as an HID (high intensity discharge) headlamp or a tungsten halogen headlamp that includes a plastic lens. In such cases the road dirt on the surface of a plastic lens covering a high temperature, high intensity discharge lamp such as is the case with HID headlamps can be literally baked onto a lens so that the outer surface of the lens becomes less light transmissive over time even after a washer cleaning cycle or cycles.


B)$. The “isospin” $\vec\Sigma$ is defined as direct products of Pauli matrices ${\bf \hat\sigma}$ (acting in the sublattice space) and ${\bf
  \Pi}$ (acting in the valley space $K_\pm$): $\Sigma_{x(y)}=\Pi_z\otimes\hat\sigma_{x(y)}$.

In the basis of plane waves $\widehat {\cal H}_0$ is a $4\times 4$ matrix that can be diagonalized by a unitary transformation $\hat R^{-1}\widehat{\cal H}_0\hat R=
{\rm diag}[E_{\alpha,\xi}({\bf p})]$. The resulting eigenvalues are $$\label{evwf}
E_{\alpha,\xi} = \alpha vp s_\xi; \; s_\xi=
\sqrt{1-2\xi pWv^{-1}\cos3\varphi_{\bf p} +p^2W^2v^{-2}},$$ where $\xi = \pm 1$ denotes the two valleys, $\alpha = \pm 1$ is the chirality and distinguishes between the conductance ($\alpha = 1$) and valence ($\alpha = -1$)bands, and $\varphi_{\bf p}$ is an angle between the momentum ${\bf p}$ and the $x$-axis ($\tan\varphi_{\bf p} = p_y/p_x$).

The electron field operator can be written in the basis ofmotif \[[@B25]\] which comprises residues 132--225 of human alkylglycerol mono-oxygenase, the catalytic core of the enzyme, we then selected mutants of all glutamate and aspartate residues (E137A, E152A, D153A, E203A and E212A) and an additional histidine mutant (H189A), which all had detectable residual activity. We tested these mutants for their concentration-dependence of alkylglycerol mono-oxygenase activity with respect to tetrahydrobiopterin and 1-*O*-pyrenedecyl-*sn*-glycerol. Cell extracts expressing wild-type enzyme were chosen as controls. Although no significant changes could be detected in the affinity of mutants towards the substrate ([Figure 3](#F3){ref-type="fig"}A), mutant E137A had an 18.3-fold increased *K*~m~ value for tetrahydrobiopterin in comparison with wild-type (33.5±8.79 μM compared with 1.83±0.38 μM; *n*=3; *P*\<0.05; [Figure 3](#F3){ref-type="fig"}B). [Figures 3](#F3){ref-type="fig"}(C) and [3](#F3){ref-type="fig"}(D) show the original data for tetrahydrobiopterin-dependence of the activity in E137A mutant and wild-type enzymes,


the presence of 5 mM tetrahydrobiopterin. Activity of the lowest phenanthroline concentration was set to 100%. Under standard assay conditions with 5 mM tetrahydrobiopterin, the E286A mutant showed 7.9±1.4% of enzymatic activity compared with wild-type phenylalanine hydroxylase. Results are means±S.E.M. (*n*=3).](bic886i004){#F4}

Iron affinity of wild-type and E286A phenylalanine hydroxylase
--------------------------------------------------------------

Since iron affinity of the phenylalanine hydroxylase E286A mutant which also displays a highly increased *K*~m~ value for tetrahydrobiopterin (\[[@B26]\], and see the Discussion) had not been investigated so far, we investigated iron binding of this mutant in comparison with the wild-type enzyme with a similar phenanthroline competition activity assay using recombinant *Escherichia coli*-expressed enzymes for comparative purposes ([Figure 4](#F4){ref-type="fig"}B). Both wild-type and mutant phenylalanine hydroxylase were inhibited by phenanthroline. The corresponding IC~50~ values for phenanthroline were 6.37±0.23 μM for the E286A mutant phenylalaninehydroxylase and 15.7±1.51 μM for the wild-type phenylalanine hydroxylase (*n*=3 each; *P*\<0.01). Thus the E286A mutation in phenylalanine hydroxylase led to an approximately 2.5-fold decrease in iron affinity.

*Ab initio* predicted structure of alkylglycerol mono-oxygenase
---------------------------------------------------------------

[Figure 5](#F5){ref-type="fig"} shows the structure of residues 37--205 modelled by Rosetta Membrane. Even though no information on residues critical for protein function were used for model generation, all residues leading to a total loss of enzyme activity upon alanine mutation are found in close spatial proximity. Moreover, all five histidine residues of the eight-histidine motif covered in the modelled region, as well as the highly conserved His^201^, form a shallow cavity ready to accommodate a catalytically active iron cluster that could not be included in the actual model. Glu^137^, the residue involved in the interaction with


tetrahydrobiopterin, is found in a flexible loop region close to the histidine motif at the surface of the predicted partial structure accessible for the cofactor.

![Rosetta *ab initio* predicted partial structure of alkylglycerol mono-oxygenase\
Three-dimensional structure of alkylglycerol mono-oxygenase residues Pro^37^--Ile^205^ including five transmembrane regions predicted using the Rosetta membrane method \[[@B7]\] visualized using PyMOL (<http://www.pymol.org>). (**A**) Helical regions are shown in tube representation, and residues leading to inactivation of alkylglycerol mono-oxygenase upon mutation to alanine and Glu^137^ crucial for tetrahydrobiopterin binding are highlighted as sticks. (**B**) Close-up of the suspected active site of alkylglycerol mono-oxygenase. All five modelled histidine residues of the eight-histidine motif as well as Glu^137^ are found in close spatial proximity forming a shallow cavity ready to accommodate an iron cluster.](bic886i005){#F5}

DISCUSSION
==========

The present paper is a firstWhat is the nearest to 0 in m, f, -1/8?
-1/8
Let g = -0.27046 - 3.72954. What is the nearest to 1444 in g, 4, 1/9?
4
Let y(q) be the third derivative of -7*q**5/60 + 7*q**4/12 - q**3/6 + 59*q**2 + 2. Let o be y(2). Let n = -6 - -4. What is the nearest to n in 4/5, 0.3, o?
o
Let k = 522 + -6788/13. What is the nearest to 1 in 0, k, -3.2, -5/3?
0
Let k be 38/76 + 4/(-10). Let m = -6429/20 + 1257/4. Let v = m - -22/3. Which is the closest to 1?  (a) k  (b) v  (c) 3
b
Let v = 27.28 - 27.3. Which is the nearest to v?  (a) -0.5  (b) -1/3  (c) 1/2
b
Let