提交任务
  4:     auditExecutorService.submit(() -> {
  5:         // 循环，直到停止或线程打断
  6:         while (!auditStopped.get() && !Thread.currentThread().isInterrupted()) {
  7:             try {
  8:                 // 获得队首 InstanceConfigAuditModel 元素，非阻塞
  9:                 InstanceConfigAuditModel model = audits.poll();
 10:                 // 若获取不到，sleep 等待 1 秒
 11:           return instanceService.findInstance(instance.getAppId(), instance.getClusterName(),
                    instance.getDataCenter(), instance.getIp()).getId();
        }
    }
     ```
     * 🙂 代码比较简单，胖友看下注释。
 * ============ InstanceConfig 相关 ============
 * 第 15 行：拼接 `instanceConfigReleaseKeyCache` 的 KEY 。
 * 第 18 行：调用 `Cache#getIfPresent(key)` 从缓存 `instanceConfigReleaseKeyCache` 中获得 `cacheReleaseKey` 。
 * 第 19 至 23 行：若 `releaseKey` 相当，说明**无更新**，跳过。
 * 第 25 行：更新对应的 `instanceConfigReleaseKeyCache` 缓存。
 * 第 26 至 29 行：调用 `InstanceService#findInstanceConfig(...)` 方法，获得 InstanceConfig 对象。相比 Instance 来说，InstanceConfig 存在**更新**逻辑。
 * 第 31 至 49 行：若 InstanceConfig 已经存在，进行更新。
    * 第 34 至 37 行：若 `releaseKey` 发生变化，设置需要更新的字段 `configClusterName` `releaseKey` `releaseDeliveryTime` 。**注意**，`releaseDeliveryTime` 配置下发时间，使用入队时间。


for life after baby. The night I brought…

Every night, the bedtime routine for my child begins really well but somewhere after many stories, some unreasonable requests and a lot of wriggling later, it spirals into insanity. I have to fight monsters who appear only when it's time to close the eyes. I have to oblige to several requests for water until I know I'm being tricked and then have to put on a stern face. There are times when I have to lay still and lifeless for hours, like a mummy, hoping my son will fall asleep only to creep out later to find him wide awake. Then there are nights when he will cry and get clingy simply because he wants no one else but ME toand bear testement of being ripped.

A couple months back we began 'reading time' - any time during the day when he would haul all his books out and place them on a table and pretend he was reading.

Last night after almost a year of bedtime stories and for the first time he chose the book he wanted and brought it to me!

Reading..it's never too early to start and never too late to begin- Maria DeRosario

Picture: He's learning to point out alphabets and the newspaper has been a very cool start!

Boston is my son's favourite Teddy Bear since he was a year and half. We've had many adventures together and so for the first time I bring to you " The Adventures of Boston the Bear".

For his first adventure,


Boston decided that the best place to go would be: well, space. The planets and galaxy have always fascinated him. So join him as he jets off in his splendid Space Rocket, whirls around the plantes,the moon and decides if he would want to live on Mars someday.

A short adventure filled with excitement and written for my adventurous son!by at least 20$\%$. With a threshold flux ratio of 1.2, restricting our analysis to $>5\sigma$ detections at 16$\mu$m, and using a $3\sigma$ limit on 24$\mu$m non-detections, the number of silicate-absorbed ULIRG candidates is 36. This defines a strong upper limit of $\sim$920 sources deg$^{-2}$, on the population of silicate-absorbed ULIRGs at z $\sim$ 1–1.8. This source count is about half of the total number of sources predicted at z $\sim$ 1–2 by various phenomenological models. We note that the high 16/24$\mu$m colors measured cannot be reproduced by any of the mid-IR spectral energy distributions assumed by these models, which points to the strong limitations currently affecting our phenomenological and theoretical understanding of infrared galaxy evolution.'
author:
- 'M. M. Kasliwal, V. Charmandaris, D. Weedman, J.R. Houck, E. Le Floc’h,


varies from 11.2 (type B0,1V) to 17.5 (type M,late V) [see @Wainscoat92]. This corresponds to a range in R band magnitude of 11.3-15.7. Clearly, the R-mag of all our sources is 4–5 mags fainter. Thus it is highly unlikely that we are observing faint main sequence stars. Red giants, embedded protostars or asteroids are also improbable contaminants because of the high Galactic and high Ecliptic latitute of the Bootes field (b$\sim$67$\deg$ and l$\sim$45$\deg$ respectively) as well as the multiple epochs of the MIPS24 catalogue.

Since there are no direct spectroscopic observations available for at least a fraction of the galaxies of our sample, we cannot cross-calibrate the method using broadband colors and the exact redshift of the source. However, @Teplitz05 performed a similar study around Hubble Deep Field North,Balkrishna Mohoni

Balkrishna Mohoni (15 April 1901 – 7 April 1980) was an Indian cricket umpire. He stood in eleven Test matches between 1949 and 1956. Mohoni played 11 first-class matches for Maharashtra, between 1934/35 and 1941/42.

See also
 List of Test cricket umpires

References

Category:1901 births
Category:1980 deaths
Category:Cricketers from Pune
Category:Indian Test cricket umpires
Category:Indian cricketers
Category:Maharashtra cricketers


technology gives you the option of a reduced flow rate with no compromise on enjoyment. Thanks to the GROHE QuickFix system it’s swift and straightforward to install. The slim profile makes it easy to keep clean, and the dazzling, durable GROHE StarLight chrome finish will look pristine for years to come. With a slim, circular design perfect for that minimalist, streamlined look, this set is bound to enhance your bathroom scheme.done using something like this
SELECT date,
   visits.ip
FROM visits
   INNER JOIN (SELECT ip
               FROM   visits
               GROUP  BY ip
               HAVING COUNT(id) > 1) dup
           ON visits.ip = dup.ip

However I couldn't figure out how to implement counting the records into this one.
How can I make the second and the third query?
I am also open to PHP solutions

A:

I would rewrite your first query to use the date() function to remove the


the March 2015 inventory total, and MSCI’ estimated it represents a 2.3-month supply of steel products at the current delivery rate.

Canadian service centers shipped 392,200 tons of steel during March, 1.2% less than during February and 11.3% less than during March 2015. The daily shipment rate fell 5.8% from February, to 17.8 tons/day. The January-March shipment total is 1.18 million tons, but that figure is .47% less than the three-month total for 2015.

Inventories of steel at Canadian service centers fell 1.9% from February to 1.19 million metric tons, which is 28.8% lower than the March 2015 inventory total. It is estimated that Canadian service centers are carrying a 3.0-month supply of steel at the current rate of deliveries.

Aluminum products shipments from U.S. service centers during March rose 7.1%March is a good month to think about the new Harriet Tubman Underground Railroad National Monument in Eastern Maryland, the first national park site named after an African American woman. Harriet was born in March, made up her mind to flee slavery in March and eventually died in March in 1913 at the age of 91. When we heard about the creation of the new park, we decided we wanted to find "Harriet" and headed down to the Eastern Shore fully aware that the new visitor center had barely broken


[Pharmacokinetic Problems with Psychotropic Drug Combination Therapy].
The pharmacokinetics of drugs vary markedly among patients. It is also necessary to aware that pharmacokinetics can change within the same patient. A typical example is drug interactions. Psychotropic drugs generally have a high plasma protein binding rate, which may increase the effects of medications taken concomitantly. Furthermore, psychotropic drugs often competitively inhibit the enzymes metabolizing drugs, and thereby increase the blood levels of concomitantly administered medications. On the contrary, there are also psychotropic drugs, which induce metabolic enzymes and thereby lower the blood levels of concomitant medications. As the number of drugs administered increases, these interactions become more complicated, creating increasing difficulty in estimating clinical effects. Therefore, multidrug combination therapy is not, based on pharmacokinetic considerations, recommended.FAQs

We get loads of questions about who we are and what we do. Many people think this is our full-time job, but its far from it. All of us work outside of Ultra168 and we do this just for fun… but for some more detailed information, we’ve prepared some Q&As below to give you more of an idea about who we are and what we’re about. If you still have any questions, feel free to email us at ultra168@hotmail.com

Who is Ultra168?

Ultra168 was set up after Darrel, Andrew and Terry embarked on their first grand adventure to record the fastest time across the entire length of the GNW. They wanted somewhere to record this trip, and Ultra168 was born as a team of mates who simply enjoyed running together.


Computer chips are often connected to an underlying substrate using a series of solder balls, solder bumps, or other solder joints or the like (often called first level interconnect solder bumps) that are positioned between the chip and the substrate and then heated to the point where the solder reflows. During chip attach there is a significant thermal expansion mismatch between the substrate and the chip (also referred to herein as a die) because of the large difference in their coefficients of thermal expansion (CTE). A typical organic substrate has a CTE that is perhaps five or six times greater than the CTE of a silicon die. Accordingly, when the die is placed on the substrate and heated to the solder reflow temperature, the expansion experienced by thearray[currentRow].length) {
                currentColumn = 0;
                currentRow++;
            }
            if (currentRow == array.length -1 && currentColumn == array[currentRow].length - 1) {
                throw new NoSuchElementException();
            }
            return array[currentRow][currentColumn++];
        }
    };
}

Alternatively you can


Development of high throughput optical sensor array for on-line pH monitoring in micro-scale cell culture environment.
On-line pH detection of cell culture environment is necessary in a bioprocess or tissue engineering. Devices by means of electrochemical mechanisms for this purpose have been reported to be less suitable compared with optical-based sensing principles. More recently, some non-invasive optical sensing systems have been proposed for online pH monitoring of cell culture environment. However, these devices are not for multi-target pH monitoring purpose, and are large in scale and thus not appropriate for the pH monitoring at a micro scale such as in microbioreactor or microfluidic-based cell culture platform. To tackle these issues, an optical fiber sensor array for on-line pH monitoring was proposed using microfluidic technology. The working principle is basedIn this week’s round-up, new seasons are on the way for series on U.S. nets Bravo, Spike TV and ABC, as well as Canada’s food and lifestyle net Gusto TV.

Bravo Media has renewed The Real Housewives of Orange County for a 10th season, which kicks off on June 8 at 9 p.m. EST/PST. The Evolution Media-produced series follows the everyday drama and relationships between housewives Vicki Gunvalson, Tamra Judge, Heather Dubrow, Shannon Beador and new recruit Meghan King Edmonds.

Meanwhile, ABC is bringing back The Bachelorette for its 11th edition with a two-night special, airing first on Monday (May 18) at 9 p.m. EST/PST, and then on Tuesday (May 19) at 8 p.m. EST/PST. This season features 25 men competing for the affection of bachelorettes Britt and Kaitlyn. The


is the plot and setting. For older gamers, the usual JRPG plot might be a bit too much to stomach, all with saving the world with bikini girls. Pokemon on the other hand has silly plots, but they are very down to earth in comparison.
On the other side of the plot, its just you on your journey to become the best trainer. And not just in the game, but among your friends and lately, across the world.
Collecting things and "getting better" are things, everyone can relate to. Also, the games are most often rather easy and can be played by everyone (not talking about mastering it), so it definitly has a wide range of appeal.

While the whole franchise (Anime, Merch, Spin offs etc) might not be anyones cupmedia web pages. Sometimes, brands may provide different content
to different audiences. However, audiences may vary widely in their
preferences for how content is provided to them. For example, one
audience may prefer an image with the content, while another audience may
prefer no image. If an audience has a preference for images, but the
brand does not provide images, then the audience may lose interest in the
brand. As a result, the uninterested reader may turn to a different a
different brand and/or media property in the future. Accordingly, the way
in which content is provided to an audience and the preferences of that
audience can impact an engagement level of the audience; referral and/or
retention rates; return on investment, or the like. It is with respect to
these considerations and others that the present invention has been


may be provided to the channel based on the
determined test/outcome. In at least one embodiment, the content may be
modified based on contextual recommendations that correspond to the
determined outcome. In another embodiment, the channel that the content
is provided may be selected based on the determined outcome. In some
embodiments, each piece of a plurality of content may employ a test,
where a subset of the plurality of content is employed with a first
outcome and a different subset is employed with a second outcome. In at
least one embodiment, an outcome to employ with the content may be
randomly selected such that each of the outcomes of the test is selected
a substantially similar number of times.

[0025] In at least one embodiment, actions associated with the plurality
of content may be monitored and/or analyzed. These monitoredIn some
embodiments, client devices 102-105 may include virtually any portable
personal computing device capable of connecting to another computing
device and receiving information such as, laptop computer 103, smart
mobile telephone 104, and tablet computers 105, and the like. However,
portable computing devices are not so limited and may also include other
portable devices such as cellular telephones, display pagers, radio
frequency (RF) devices, infrared (IR) devices, Personal Digital
Assistants (PDAs), handheld computers, wearable computers, integrated
devices combining one or more of the preceding devices, and the like. As
such, client devices 102-105 typically range widely in terms of
capabilities and features. Moreover, client devices 102-105 may access
various computing applications, including a browser, or other web-based
application.

[0029] A web-enabled client device may include a browser application that
is configured to receive and to send web pages, web-based messages, and
the like.


may determine which tests/outcomes
are to be employed with a piece of content. In other embodiments, SDSD
114 may enable a marketing administrator to select, add, and/or modify
content based on the outcomes (i.e., the contextual recommendations). In
at least one embodiment, SDSD 114 may be enabled to communicate with TMSD
116 to access tests and/or corresponding outcomes. In some other
embodiments, SDSD 114 may schedule and/or re-schedule tests to be
employed with content.

[0042] Devices that may be arranged to operate as SDSD 114 include various
network devices, including, but not limited to personal computers,
desktop computers, multiprocessor systems, microprocessor-based or
programmable consumer electronics, network PCs, server devices, network
appliances, and the like.

[0043] Although FIG. 1 illustrates SDSD 114 as a single computing device,
the invention is not so limited. For example, one or more functions of
the SDSD 114 maybe distributed across one or more distinct network
devices. Moreover, SDSD 114 is not limited to a particular configuration.
Thus, in one embodiment, SDSD 114 may contain a plurality of network
devices. In another embodiment, SDSD 114 may contain a plurality of
network devices that operate using a master/slave approach, where one of
the plurality of network devices of SDSD 114 operates to manage and/or
otherwise coordinate operations of the other network devices. In other
embodiments, the SDSD 114 may operate as a plurality of network devices
within a cluster architecture, a peer-to-peer architecture, and/or even
within a cloud architecture. Thus, the invention is not to be construed
as being limited to a single environment, and other configurations, and
architectures are also envisaged.

[0044] One embodiment of TMSD 116 is described in more detail below in
conjunction with FIG. 3. Briefly,


may contain a plurality of network
devices. In another embodiment, TMSD 116 may contain a plurality of
network devices that operate using a master/slave approach, where one of
the plurality of network devices of TMSD 116 operates to manage and/or
otherwise coordinate operations of the other network devices. In other
embodiments, the TMSD 116 may operate as a plurality of network devices
within a cluster architecture, a peer-to-peer architecture, and/or even
within a cloud architecture. Thus, the invention is not to be construed
as being limited to a single environment, and other configurations, and
architectures are also envisaged.

Illustrative Client Device

[0047]FIG. 2 shows one embodiment of client device 200 that may be
included in a system implementing embodiments of the invention. Client
device 200 may include many more or less components than those shown in
FIG. 2. However, the components shownalso be provided by an external power source, such as an
alternating current (AC) adapter or a powered docking cradle that
supplements and/or recharges a battery.

[0050] Client device 200 may optionally communicate with a base station
(not shown), or directly with another computing device. Network interface
236 includes circuitry for coupling client device 200 to one or more
networks, and is constructed for use with one or more communication
protocols and technologies including, but not limited to, GSM, CDMA,
TDMA, GPRS, EDGE, WCDMA, HSDPA, LTE, user datagram protocol (UDP),
transmission control protocol/Internet protocol (TCP/IP), short message
service (SMS), WAP, ultra wide band (UWB), IEEE 802.16 Worldwide
Interoperability for Microwave Access (WiMax), session initiated
protocol/real-time transport protocol (SIP/RTP), or any of a variety of
other wireless communication protocols. Network interface 236 is
sometimes known as a transceiver, transceiving device, or network
interface card


employed to store social networking information
including address books, buddy lists, aliases, user profile information,
or the like. Further, data storage 208 may also store message, web page
content, or any of a variety of user generated content. At least a
portion of the information may also be stored on another component of
network device 200, including, but not limited to processor readable
storage media 230, a disk drive or other computer readable storage
devices (not shown) within client device 200.

[0061] Processor readable storage media 230 may include volatile,
nonvolatile, removable, and non-removable media implemented in any method
or technology for storage of information, such as computer- or
processor-readable instructions, data structures, program modules, or
other data. Examples of computer readable storage media include RAM, ROM,
Electrically Erasable Programmable Read-only Memory (EEPROM), flash
memory or other memory technology, Compact Disc Read-onlymay return to a calling process to
perform other actions.

[0106] Although process 500 employs two outcomes of a test, embodiments
are not so limited; but rather, a similar process may be employed for
comparing metrics for a plurality of different outcomes (e.g., employ
process 500 for each possible combination of two outcomes and stack
ranking the metrics to determine the preferred outcome).

[0107]FIG. 6 illustrates a logical flow diagram generally showing an
alternative embodiment of a process for providing content to a channel
based on a determined test and an associated outcome. In some
embodiments, process 600 of FIG. 6 may be implemented by and/or executed
on a single network device, such as network device 300 of FIG. 3. In
other embodiments, process 600 or portions of process 600 of FIG. 6 may
be implemented by and/or executed on a


After providing adequate information to create the
test, the marketing administrator can save the test, which may enable the
test to be selected/determined at block 404 of FIG. 4.

[0136] Although interface 700 illustrates text boxes and check boxes for
input, embodiments are not so limited; rather other forms, types/methods
of input, or the like may be employed. For example, in other embodiments,
outcomes 704 and 706 may include buttons that, when activated, open
separate windows for the marketing administrator to enter outcomes.

[0137]FIG. 8 shows a use case illustration of an embodiment of a
graphical user interface that may be employed to select contextual
recommendations to apply to content. In at least one embodiment,
interface 800 may be employed after a piece of content is selected (e.g.,
at block 402 of FIG. 4) and a test/outcome is determined (e.g.,negotiations were proceeding smoothly, with the Russians so far abiding by the terms of the deal, under which rebels would hand over weapons, and fighters who do not wish to live under state rule would be evacuated.

“Everyone is committed to the agreements,” said rebel official Abu Jihad, adding rebels had already begun since late Wednesday handing over their heavy weapons.

Two armoured vehicles with senior Russian officers entered the area of al Shayah in the devastated old city and began talks with commanders from the Free Syrian Army (FSA) on implementing the terms of the deal.

One rebel official said fighters hoped the Russians would keep their pledge to maintain a permanent Russian military police presence inside the enclave to protect civilians and former rebels who decide to remain under


invariant under scalings of the metric: if $g \rightarrow \lambda g$, $\lambda\,\in\,\mathbb{R}_{>0}$, then $\mathrm{Rc}(\lambda g) = \mathrm{Rc}(g),$ but $\mathrm{Rm}^2(\lambda g) = \frac{1}{\lambda} \mathrm{Rm}^2(g)$, and consequently $$2\mathrm{Ric}(\lambda g )\,+\,\frac{\alpha}{2}\,\mathrm{Rm}^2(\lambda g)\,=\,
2\mathrm{Ric}(g )\,+\,\frac{\alpha}{2}\,\lambda^{-1}\,\mathrm{Rm}^2(g)\;.$$ This is at variance with what happens for the Ricci flow, where one has manifestly parabolic space and time scaling symmetry, which are of basic importance in the geometric applications of the theory. The lack of scaling invariance is a source of a number of delicate problems in the analysis of the RG-2 flow. This is already evident when dealing with the condition assuring its (weak)–parabolicity, according to which the flow exists (and is parabolic) provided that [@GGI2], [@cremaschi], [@oliy09] $$\label{parcond0}
1\,+\,\alpha\,\mathcal{K}_{(g)}[\sigma]\,>\,0\;,\;\;\;\;\;\forall \,\sigma\in Gr_{(2)}(TM)\;,$$ where $\mathcal{K}_{(g)}[\sigma]$ denotes the sectional curvature of the initial $(M, g)$ along the plane $\sigma\inGr_{(2)}(TM)$, and $Gr_{(2)}(TM)$ is the Grassmannian of 2-planes in $TM$; however, under the scaling action $g \rightarrow \lambda g$ we get $$\begin{aligned}
\label{scalInv}
\mathcal{K}_{(\lambda g)}[\sigma(X,Y)]\,=\,\lambda^{-\,1}\,\mathcal{K}_{(g)}[\sigma(X,Y)]\;.\end{aligned}$$ It follows that if we assume that the condition (\[parcond0\]) holds for the manifold $(M, g)$, then on the rescaled manifold $(M, \lambda g)$ the analogous condition may easily fail as soon as $\mathcal{K}_{(g)}[\sigma]\,< 0$ and $\lambda$ is small enough. The fact that (weak)–parabolicity of (\[RG2flow\]) depends on the *size* of the manifold is a somewhat unsatisfactory feature. One may argue that from a PDE point of view this behavior cannot be formally ruled out; nonetheless, one would like a deeper rationale for the fact that a geometric flow, driven by local curvatures, changes nature abruptly as a function of the *overall size* of the


besides $\alpha_{g_0}\,=\, \alpha_{g(t)}$, we have used $\mathrm{Ric}(g(t)) = \mathrm{Ric}(g_0)$ and $\mathrm{Rm}^2(g(t))\,=\,\sigma(t)^{-\,1}\,\mathrm{Rm}^2(g_0)$. Since $g_0$ is of constant curvature, we can write $\mathrm{R}_{ij}(g_0)\,=\,K\,(n-1)\,(g_0)_{ij}$ and $\mathrm{Rm}^2_{ij}(g_0)\,=\,2K^2\,(n-1)\,(g_0)_{ij}$. Hence, corresponding to the assumed scaling evolution for the metric, the RG-2 flow takes the form (factoring out a common $g_0$) $$\frac{d}{d t}\sigma(t) \,= \, -2K\,(n-1)\, -\alpha_{g_0}\,\sigma(t)^{-\,1}\, K^2\,(n-1)\,,$$ which is implicitly solved by the Lambert W function construction that was developed in [@GGI3], *i.e.*, by scaling factors $\sigma(t)$ which satisfy $$\sigma(t) \,= \, -2K\,(n-1)t\,+\,1\, +\,\frac{\alpha_{g_0}\,K}{2}\,\ln\,\left|\frac{2\sigma(t)\,+\,\alpha_{g_0}K}{2\,+\,\alpha_{g_0}K} \right|\;.$$

These remarks explicitly show that the variegated nature of the soliton structures for the standard RG-2 flow is not caused by the fact that the flow is not scale invariant. The complex structure is indeed found also for the scale-invariant RG-2 flow. It is the geometric interplay with the $\alpha_g\mathrm{Rm}^2$of the instantaneous and synchronous variation of the Perelman functional along the Ricci flow direction and along a $\mathrm{Rm}^2$ flow direction.

In what follows, we derive an extended Perelman entropy $\mathcal{F}({g}(t), {{f}(t)}, {\xi}_g(t))$ that is a natural generalization of Perelman’s entropy by exploiting the gauge freedom related to the gradient vector field ${\xi_g}$. Although our strategy emphasizes, as in Perelman’s analysis of the Ricci flow [@Perelman], the interplay between the diffeomorphism group and the RG-2 flow, it has aspects that are in the spirit of Chow and Lu’s suggestion. We replace their two–flows splitting with the full RG-2 flow coupled to a corresponding auxiliary flow governing the gauge drift vector field ${\xi_g}$ associated to the measure $d\omega$. It is the latter that allows to take into account the contribution


existence result for the geometric flows associated to the $3$–loop and $4$–loop curvature contributions, (where explicit curvature expressions are available–see *e.g.* [@tseytlin]) is an extremely demanding task. Were this possible, one could presumably use the parabolic Fokker–Planck evolution (\[wabevol\]) (with the quadratic source term $\frac{\alpha_g^2}{32}\,\left|\mathrm{Rm}^2(\overline{g}(t),\,\overline{\xi}_{g(t)})\right|^2$ replaced by the corresponding $k$–th order curvature terms present at the given loop approximation) in order to control the monotonicity of the associated energy functional.

IS THE RG-2 FLOW A GRADIENT FLOW?
=================================

The expression (\[PerRG2F\]) directly shows that although the modified Perelman entropy $$\label{firstModPer}
\mathcal{F}_{(1)}(\overline{g}, d\overline{\omega},\,\overline{\xi}_{g})\,:=\,
\int_M\, \left(\mathrm{R}^{Per}(\overline{g}(t))\,+\, \left|\overline{\xi}_{g(t)}\right|^2\right)\,d\overline{\omega} (t)\;,$$ is monotonic, the (modified) RG–2 flow (\[DToutPer\]) is not a gradient flow with respect this functional. Actually, the functional with respect to which the (DeTurck modified) RG–2 flow is gradient is a rather non–trivial modification of (\[firstModPer\]):g(t))$.

[^3]: At any given instant $t$;when working at fixed time $t$ we drop the explicit time dependence in what follows.

[^4]: See,*e.g.* [@flowers0] Lemma 5.22 for the analogous case of Perelman’s $\lambda(g)$ functional


cameras, premium laptop, handheld gimbal or drone, the Chicago collection is for you: it’s a photography bag designed to stay with you throughout your day to day experiences of life in the city, on the most epic photo and video shoots, and on to exciting weekend getaways.

You’ll love the carefully selected materials and design details that make this pack built to last: Kevlar® yarn fabric on the bottom part of the bag for extreme protection and durability, coated fabric with matte finish, Duraflex® buckles and YKK zippers. Moreover, external fabric is treated to make it water repellent and a rain cover is also supplied in case of extreme weather conditions.

Both the Chicago camera backpack Medium and Small models feature a removable photo insert that enables a switch from4600523 prime?
False
Is 968748719 a composite number?
False
Is 302007317 prime?
True
Is 445410299 prime?
True
Is 135565483103 a prime number?
False
Is 82612531907 prime?
True
Is 5818190021 a prime number?
False
Is 13799195591 a prime number?
True
Is 19236988283 a prime number?
True
Is 2872167299 prime?
True
Is 1791149867 prime?
False
Is 52318697 a prime number?
False
Is 25155323 prime?
True
Is 36806569019 prime?
False
Is 129167894605 composite?
True
Is 1746318019 composite?
False
Is 2690886923 a prime number?
True
Is 6646091899 prime?
False
Is 42156971427 a prime number?
False
Is 3227747629 a composite number?
True
Is 4031323337 prime?
False
Is 9339564187 a prime number?
True
Is 126397507703 composite?
False
Is 2271092039 a composite number?
True
Is 2651668991 prime?
True
Is 133536857 a composite number?
False
Is 12185686923 composite?
True
Is 279911758297 a composite number?
False
Is 139490249 a composite number?
True
Is 695112109 composite?
False
Is 3596190817 a prime number?
False
Is 3408517633 a composite number?
False
Is 4390211249 composite?
False
Is 12471383129 a composite number?
True
Is 251024129 prime?
True
Is 36236918779 a composite number?
True
Is 84894204007 a composite number?
False
Is 438077359 prime?
True
Is 251069882053 a composite number?
False
Is 1992529879 a composite number?
False
Is 1911765281 prime?
True
Is 4469003821 a composite number?
False
Is


1451779561 composite?
False
Is 15091559911 composite?
True
Is 27371821385 prime?
False
Is 5737014302 a prime number?
False
Is 22040518559 composite?
False
Is 13629463319 a prime number?
True
Is 55828913233 a composite number?
True
Is 58239069061 a composite number?
True
Is 95270995151 composite?
False
Is 1273014917 a composite number?
True
Is 8493095419 a prime number?
True
Is 19597447223 composite?
True
Is 2306387495 a composite number?
True
Is 13591744003 a composite number?
True
Is 1166709097 a prime number?
False
Is 21324576239 a composite number?
False
Is 7254185459 a composite number?
False
Is 214094563 prime?
True
Is 24739249 a prime number?
False
Is 624552161 composite?
False
Is 92547410129 composite?
True
Is 1703099647 a composite number?
False
Is 2695270723 a composite number?
False
Is 822244073 prime?
False
Is 264511301051 composite?
True
Is 121777547 composite?
False
Is 338966651 a composite number?
True
Is 25368713 composite?
False
Is 1283281939 prime?
True
Is 498319721 prime?
True
Is 78441443551 a prime number?
False
Is 12503000371 a composite number?
Tris not necessary and, indeed, is manifestly improper, so I give it no weight whatsoever. It certainly does not raise any genuine issue of fact.
However, Ms. Flaherty's affidavit sets out Plaintiff's arguments about the "more favorable term" issue more clearly than any other papers submitted by Plaintiff. I therefore consider Ms. Flaherty to be acting as co-counsel for Plaintiff and advancing an arguments on behalf of her "clients."
Plaintiff's position is as follows:
(1) The Shareholders' Agreement expressly precludes Reliance from selling or agreeing to sell its Command Securities to GCM on terms more favorable to GCM than those previously offered to Vassell in the January 16 Notice, without first reoffering the shares to Vassell.
(2) The Financing Agreement between CIT and Command (Section 10.1(j)) states that an "Event of Default"


an express statement to that effect in the April 23 Agreement did no more than restate a condition that applied to any and every potential purchaser of Reliance's stock.
Second, and more important, there was no need to expressly state that CIT had to approve Vassell's purchase of Reliance's shares in the offer Reliance made to him, because Vassell was already pre-approved as the purchaser of those shares. CIT had expressly agreed to finance Command's operations as long as Vassell was running Command and remained a substantial shareholder. Nothing Vassell did that entrenched his control over the company (such as buying Reliance's stock, which would have made it impossible to interfere with his management of the company) could result in an Event of Default, because CIT had already indicatedCIT approval condition, GCM put itself in a worse position than Vassell, since GCM now bears the risk that Vassell never would have faced  the risk that CIT will decide not to finance Command if Vassell is no longer in charge.
Under no circumstances, then, can the terms of the Purchase Agreement be deemed materially more favorable to GCM than the terms of the offer made to Vassell.

D. The Purchase Agreement was Enforceable as of April 23, 2004

Plaintiff also appears to argue that the Purchase Agreement, which was dated and executed on April 23, 2004, did not actually become a valid and enforceable agreement until May 20, 2004  the date when GCM waived the condition requiring CIT to approve the sale  because until that date, it


was possible that GCM would not purchase the shares. As a result, Vassell claims, the 90 day period expired without Reliance having entered any agreement to sell its Command stock, thus requiring it to re-offer the shares to him.
This argument ignores the most basic principles of contract law. The Shareholder Agreement gave Reliance 90 days following Vassell's refusal to buy the stock to enter into an agreement. A valid and binding executory contract was entered into on April 23, 2004. Indeed Vassell so admits in his Rule 56.1 Statement (¶ 13), which is binding on him in this litigation.
It is true that GCM's obligation to complete performance under that Agreement  to close on the purchase of the stock  was the fulfillment of a condition precedent toRule 16 conference to deal with what (if anything) is left of the case, including GCM's counter/cross claims for damages. Counsel for Reliance and Vassell are directed to notify the Court if no appeal is taken, and a Rule 16 conference will be scheduled forthwith.
This constitutes the decision of the Court.
NOTES
[1]  At oral argument, counsel for Reliance stated that only approximately 25% of its beneficial ownership consists of currently exercisable common stock. To obtain control, Reliance (and now GCM) would have to convert the preferred stock and exercise the warrants.
[2]  I reject Vassell's arguments that no such agreement was reached until either May 14 or May 20. See infra, pp. 472-73.
[3]  Plaintiff points out that some differences exist between the versions of the April 23


Q:

Enforce service layer business rules on entity property changes, or hide entity property from clients but not service?

Let's say I have a class Customer, which has a property customerType.
I have another class called something like SpecialContract, which has a Customer and some other properties.
If customer.customerType == SPECIAL, then there is a SpecialContract which has a reference to this particular Customer.
Now I realize that this is a bit hoaky, but I do not want to maintain a relationship from Customer to SpecialContract for a few reasons, one being that most of the time when working with Customers we don't need to load up SpecialContracts and all of the other data associated with SpecialContracts.  However, I do always want to know if a Customer has a SpecialContract, and this+ d*c - 30 = -a. Does 7 divide (2/4)/(a/(-504))?
True
Suppose 3*t = -2*h + 36, -2*h + 7*h = 2*t + 52. Suppose 116 - h = 4*w. Suppose 6*d - w = 5*d. Is 17 a factor of d?
False
Let m(b) = -2*b**2 - 8*b - 7. Let z be m(-5). Suppose -5*k + 14 = w + 3, 4*w = -3*k + 95. Let t = w - z. Is t a multiple of 19?
False
Let p be 0 - (0 + -229 - 3). Suppose 0*r = 2*r + p. Is 16 a factor of 3 + 2*r/(-8)?
True
Let w(j) = 10*j - 17. Let a be w(13). Suppose -u = 2*c - 52, -4*c - a - 63 = -3*u. Does 7 divide u?
True
Suppose -2*x = -5*m


Mario Vušković

Mario Vušković (born 16 November 2001) is a Croatian footballer currently playing as a defender for Hajduk Split. He was included in The Guardian's "Next Generation 2018".

Career statistics

Club

Notes

References

Category:2001 births
Category:Living people
Category:Croatian footballers
Category:Croatia youth international footballers
Category:Association football defenders
Category:Croatian Second Football League players
Category:Croatian First Football League players
Category:HNK Hajduk Split playersselect the first column, or name the column by writing SELECT MIN(QuizID) AS SomeName FROM ....

A:

Or, (along with the correct answers)
you can name that column as you would like
string cmdText = "SELECT MIN (QuizID) AS mQuizID FROM dbo.QUIZ WHERE IsSent <> 1";
quizid = reader["mQuizID"].ToString();


models give an estimate of the average effect in the sample ([@bib5]). Because the size of the sample is finite, genotype and allele frequencies in the sample might deviate from the frequencies in the total population. These deviations might introduce error in the estimation of $\alpha.$ To investigate the effects of finite sample size in the presence of dominance, the estimates from the A-model (${\hat{\alpha}}_{A}$) and the AD-model (${\hat{\alpha}}_{AD}$) were compared by computing their RMSE for several scenarios.

Expectation of $\hat{\mathbf{\alpha}}$ {#s2}
--------------------------------------

If we take a random sample of $N$ individuals from a large population in HWE that has allele frequency $p,$ the expectation of $\hat{\alpha}$ can be computed using probabilities and estimates of each possible sample composition. We define $c$ as a set of variables $\left\{ {n_{0},n_{1},n_{2}} \right\}$ that< 0$ had the largest contributions to the RMSE, and samples where $\Delta n_{2} > 0$ had somewhat smaller contributions. [Figure 3B](#fig3){ref-type="fig"} also shows that $\Delta p$ contributed less to the RMSE than $\Delta n_{2},$ because there were samples where $\Delta p = 0,$ but $\delta\left( c \right)$ was relatively large.

### Additive plus dominance model: {#s9}

[Figure 3C](#fig3){ref-type="fig"} shows the squared error as a function of $\Delta n_{2},$ for the AD-model. The squared error was small and about equal for all $\Delta n_{2},$ except for $\Delta n_{2} = - 3,$ where the squared error was largest and exactly the same as with the A-model (see [Figure 3A](#fig3){ref-type="fig"}), because there were no individuals with genotype 2 in the sample. Similar to the A-model, the remaining variance (as shown by the


provided that all three genotype classes were sampled. The contribution of $\Delta p$ to the error was much smaller than the contribution of $\Delta HWE$. The AD-model was therefore more accurate than the A-model. Thus, even when the locus explained all variance (*i.e.*, H^2^ = 1), the mean RMSE decreased as sample size increased, because with larger sample sizes, deviations from HWE that considerably affect $\hat{\alpha}$ had a lower probability of occurring. Additionally, with larger sample sizes, the chance of missing one of the genotype classes was smaller, which further reduced the RMSE. The (mean) RMSE of $\hat{\alpha}$ was always smaller with the AD-model than with the A-model. The RMSE of $\hat{\alpha}$ scaled linearly with $d;$ if $d$ doubled, the RMSE also doubled. Remarkably, in the absence of(see Appendix C), which supports our expectation that effective number of sampled individuals may be smaller than the actual number of sampled individuals. Furthermore, in many studies that use genotype data, markers are removed if they show a significant deviation from HWE. The significance threshold that is used for HWE filtering, however, is often very liberal ([@bib9]). Consequently, there are still many markers left in the data that deviate from HWE and may give inaccurate estimates of average effects. As a result, we expect that the magnitude of $\Delta HWE$ simulated in this study may be similar to $\Delta HWE$ in empirical studies.

The estimation of average effects at single loci, as presented in this study, may be relevant for genome-wide association studies (GWAS). In GWAS, a large number


that Jess frequents in Japan to inquire more about her whereabouts. When Sarah tells people about Jess’s being lost into the forest, they all sternly warn her not to go. Sarah later visits a bar and is hit on by a travel writer (Taylor Kinney) , who just happens to be making a trip to the forest tomorrow complete with a tour guide. After searching all day to no avail, Sarah decides to wait behind for her sister when night falls, despite repeated warnings from the tour guide that staying alone in a haunted forest overnight is a really bad idea. Needless to say, Sarah ignores him and stays.

The Forest has some good scary imagery, but there’s not enough of it. It takes about an hour and aby tetrahedral coordination of a zinc ion at two CysXXXXCys motifs (each motif is contributed by a monomer). Through the motif, NOS binds the substrate L-arginine and cofactor tetrahydrobiopterin (BH~4~) which facilitates dimerization, substrate binding, and enzymatic function \[[@B34-antioxidants-08-00404],[@B40-antioxidants-08-00404]\]. The constitutive NOS-1 and -3, but not inducible NOS-2, are bound by calmodulin in response to increased calcium levels, which induces conformational changes to activate the enzymatic function \[[@B34-antioxidants-08-00404],[@B41-antioxidants-08-00404]\]. Each NOS monomer is composed of two distinct domains: the carboxyl-terminal reductase and amino-terminal oxygenase domains. Both domains are connected by a linker region that binds calmodulin \[[@B34-antioxidants-08-00404],[@B41-antioxidants-08-00404]\]. The reductase domain harbors a series of redox-active cofactors, NADPH, flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), which serially transfers electrons. The oxygenase domain, serving as the dimerization site that coordinates


oxidation is preceded by the oxidation of NO by molecular oxygen. This leads to formation of nitrogen oxide \[NO~2~ (oxidation state: +4)\] and N~2~O~3~ (Oxidation State: +3), which then elevates the reduction potential of the nitrosyl group by up to 10 fold \[[@B85-antioxidants-08-00404],[@B86-antioxidants-08-00404]\]. Because NO's reaction with molecular oxygen is so critical, S-nitrosylation of proteins preferentially takes place at cysteines in hydrophobic regions which could attract hydrophobic gases, NO and molecular oxygen, and enhance their reaction rate by 30--300 fold \[[@B84-antioxidants-08-00404]\].

**(4) *The target cysteine must be in the suitable environment*.** Which thiols are S-nitrosylated and what is the stability of the nitrosothiols, depend on the context and environmental factors \[[@B63-antioxidants-08-00404]\]. The accessibility of these target cysteines, which are often masked within hydrophobic regions of the protein, largely dependsin redox homeostasis, which alters the expression of their target genes to protect the cell against oxidative stress. These transcription factors include nuclear factor (erythroid-derived 2)-like 2 (NRF2); p53, nuclear factor κB (NF-κB); and hypoxia-inducible factor-1α (HIF-1α).

NRF2 is the master regulator of the transcription of antioxidant genes. In response to oxidative stress, NRF2 binds a vast range of gene promoters that harbor the antioxidant-response elements (ARE). These genes include enzymes involved in glutathione and thioredoxin systems (e.g., glutamate-cysteine ligase catalytic subunit \[GCLC\]), mitochondrial superoxide dismutase (Mn^2+^ SOD, SOD2), peroxiredoxin, HSP70 and ferritin \[[@B166-antioxidants-08-00404],[@B167-antioxidants-08-00404]\]. NRF2 is sequestered in the cytoplasm by formation of a latent complex with Klech-like ECH-associated protein 1 (Keap1), whereas dissociation from Keap1 enables nuclear translocation and activation of NRF2 \[[@B168-antioxidants-08-00404]\]. Among all other stimuli, S-nitrosylation


the nucleus upon S-nitrosylation \[[@B78-antioxidants-08-00404],[@B106-antioxidants-08-00404]\]. Transnitrosylation of DNA-PKcs upregulates its DNA repair activity in response to DNA-damaging anti-tumor agents \[[@B122-antioxidants-08-00404],[@B189-antioxidants-08-00404]\].

APE1 (also known as redox factor 1, Ref-1) is involved in the base excision repair of damaged DNA as well as regulation of cellular redox response \[[@B190-antioxidants-08-00404]\]. Although APE1 is predominantly localized in the nucleus, its subcellular localization is dynamically regulated and it could translocate to the mitochondria and cytoplasm \[[@B191-antioxidants-08-00404]\]. APE1 interacts with GAPDH which has translocated to the nucleus upon S-nitrosylation \[[@B78-antioxidants-08-00404],[@B106-antioxidants-08-00404]\]. APE1 interaction with GAPDH is not only critical for its nuclease activity \[[@B187-antioxidants-08-00404]\], but also induces transnitrosylation of APE1. Transnitrosylation of APE1 triggers its nuclear export, independent of the nuclear exporter CRM1 (see above), allowing for its translocation to different subcellular locales \[[@B192-antioxidants-08-00404]\].

7. S-Nitrosylation ofa thiol radical, NO directly reacts with it to form a nitrosothiol. (4) NO becomes oxidized by a transition metal, forming nitrosonium (NO^+^). Nitrosonium then reacts with a thiol close to the catalytic center to form a nitrosothiol.](antioxidants-08-00404-g002){#antioxidants-08-00404-f002}

![Two types of transnitrosylation reactions. (1) Cys-to-Cys transnitrosylation. (2) Metal-to-Cys transnitrosylation.](antioxidants-08-00404-g003){#antioxidants-08-00404-f003}

![S-nitrosylation of mitochondrial proteins confer resistance to oxidative stress. Mitochondrial proteins become S-nitrosylated in oxidizing environments. S-nitrosylation of proteins involved in respiration and energy production (Electron Transport Chain (ETC), adenosine triphosphate (ATP) synthesis, the citric acid (TCA) cycle and fatty acid β oxidation) leads to prevention of ROS production and protection of protein thiols in these enzymes against oxidative damage. In contrast, S-nitrosylation of proteins involved in cell death regulation (mPTP, caspases-3/-9, VDAC and Bcl-2) leads to protection of the cell


north of Tokyo, bound for Hawaii on Friday.The aircraft is one of the largest in the world, measuring 72 meters long and two stories high along its entire length. It has 520 seats -- more than double the average...more

In this week's Autoblog Podcast, Editor-in-Chief Greg Migliore is joined by Associate Editor Joel Stocksdale and Assistant Editor Zac Palmer. They kick off the podcast discussing the cars they've been driving in the office including the 2019 Buick Regal TourX, 2019 Honda Civic Touring, 2020 Kia...moresimply inserting a where clause to exclude non active things. The last line where we say recurse(root).GetEnumerator().MoveNext() is simply to trigger the recursion. There are actually zero elements in the resulting enumerable because the bottom of the recursion is reached when when the final SelectMany(f) calls item.Children and no children are returned.

Monday, March 02, 2009

Here's another post that started out as an answer to an excellent comment by Mike Boldischar:

"I'm reading through Domain Driven Design. One thing that bugs me is the idea of a "value" type. Why not just call those objects immutable entities? Maybe it's just a personal preference, but in my opinion, building "values" into the design adds little value. They only encapsulate a subset of entity values. Why not just specify immutable entities? Any


thoughts?"

The (very) simple answer is that it's more about identity than immutability. Entities have an independent identity. You can ask for an individual entity by it's ID. Value types only exist in terms of their parent entities and have no separate identity. Think of a line (Entity) made up of some points (Value), or a contact (Entity) that has an address (Value).

A common anti-pattern is to have entities with large numbers of properties of basic types, such as int or string. Often they map 1-to-1 to a database table. Individual properties or groups of basic types usually have some meaning in terms of the business and should be factored into to value types. Take this 'contact' class for example:

publicclass Contact

{

publicint Id { get; set; }

publicstring FirstName { get;tool against the target surface will wear and deform the fine and delicate points of the pin tool. This leads to errors which reduce accuracy and productivity.
An alternative technique developed by scientists employs chemical attachment of sample material to the substrate surface. In one particular process, DNA is synthesized in situ on a substrate surface to produce a set of spatially distinct and diverse chemical products. Such techniques are essentially photolithographic in that they combine solid phase chemistry, photolabile protecting groups and photo activated lithography. Although these systems work well to generate arrays of sample material, they are chemically intensive, time consuming, and expensive.
It is further troubling that neither of the above techniques provide sufficient control over the volume of sample material that is dispensed onto the surface


me to see what the effect was immediately€and I loved it.
What I didn't notice until later was the fact that the mirror I was using
had bevels on the edges that caused a slight flare on the edge in some of
the pictures. Still, it didn't bother me or the couple enough to eliminate
these photographs from their selection.

What I do for my mirror shots
is to position the subject so close to the mirror that when I lean the
person in toward the mirror the reflected image and the real person are
both almost equidistant from my camera. Thus, they are both in focus.
I just have to be careful not to put the profile of the subject against
the edge of the frame and have that be a distraction.

I turn my flash slightly in
toward225 square clicks of it. You can call in a helicopter to ferry you around the map, and you can commandeer pretty much any vehicle you find, but it’s a bit of a head-spinner all the same – particularly as the game is fairly reticent about giving you concrete pointers. If you interrogate the locals you might get a decent tip-off, but there’s no definitive answer as to where your target might be. It’s the antithesis of a linear experience like CoD 4, and as a result you’ll need a hell of a lot of patience and dedication to beat the level.

Things get even more complicated later on, and before long you’ll find yourself roaming the map with a list of 10 objectives to meet as you see


Synthesis and antimicrobial properties of monensin A esters.
The esters (2-10) of the ionophore antibiotic Monensin (1) were synthesized by four different methods, which are discussed in detail. These new esters were characterized by various spectroscopic techniques and subsequently tested in the face of their antimicrobial properties. Three derivatives (3, 8 and 10) showed activity against Gram-positive bacteria. Additionally derivative (10) exhibited a relatively low antifungal activity against Candida in contrast to Monensin A.of urinary tract infection. At that time, 10 antibiotics including quinolones, beta lactams, and cephalosporins that are known to be effective against gram-negative microorganisms were examined. We defined a past history of Foley catheterization as cases with catheterization for more than 3 days before the diagnosis of urinary tract infection \[[@B12]\].

2. Statistics
-------------

SPSS® version 12.0 (SPSS Inc. Chicago, IL, USA) was used, and p\<0.05 was considered to be statistically significant.

Factors suspected to be risk factors for the emergence of ESBL-producing bacteria were analyzed by univariate analysis, Kruskal Wallis test, and chi-square test. Afterward, a multivariate analysis was performed with all significant variables, and the odds ratio was calculated with a confidence interval of 95%.

RESULTS
=======

1. Epidemiology of ESBL-producing bacteria in UTI
-------------------------------------------------

Among the total 2,312 patients with urinary tract infection, 291


Modern surgical therapy: limb salvage and the role of amputation for extremity soft-tissue sarcomas.
Historically the surgical management of extremity soft-tissue sarcomas (ESTS) commonly involved amputation. Nowadays limb-sparing, function-preserving surgery is the standard of care for ESTS. Adjuvant therapies such as radiation therapy and chemotherapy are used selectively in an effort to minimize both local recurrence and distant spread. Less common modalities, such as isolated limb perfusion, isolated limb infusion, and hyperthermia are being evaluated to potentially expand the cohort of individuals who may be eligible for limb-sparing surgery and to improve outcomes. This article reviews the standard and evolving approaches to the management of ESTS.from complementary (epidemiological and laboratory) investigations have proved that some edible plants as a whole or their documented ingredients with antioxidant potentials possess considerable protective effects on human tumor genesis [@bib0320]. Comparable evidence has also been identified to demonstrate the chemopreventive properties of phytochemicals and compounds from diets of plant origin with free-radical chelating properties on ulcers, diabetes, memory and cognitive functions, Alzheimer disease, sexual and spermatogenesis dysfunctions, atherosclerosis and neuro-vascular disorders and several other human illnesses [@bib0325], [@bib0330], [@bib0335], [@bib0340], [@bib0345], [@bib0350]. Moreover, some medicinal herbs have been demonstrated to possess both preventive and/or healing effects. Spices are well-known as sources of natural compounds that can prevent oxidative stress and thus have a significant role in chemoprevention against degenerative illnesses [@bib0330]. The therapeutic properties of spices are


in the dark at ambient conditions for 2 h to complete the reaction. The absorbance was measured at 765 nm. Caffeic acid was used as standard and the results were expressed as mg caffeic acid/g dried extract (mg CA/g DE).

### 2.5.2. Determination of total flavonoid content {#sec0090}

Total flavonoid content was determined using aluminum chloride (AlCl~3~) according to a known method [@bib0440] using quercetin as a standard. A volume of 0.1 mL of extract was added to 0.3 mL distilled water followed by 0.03 mL of NaNO~2~ (5%). After 5 min at 25 °C, 0.03 mL of AlCl~3~ (10%) was added. After a further 5 min, the reaction mixture was mixed with 0.2 mL of 1 mM NaOH and finally, the reaction mixture was diluted to 1 mL with distilled water and the absorbance was measured at 510 nm. The results wereshifted to crushed ice bath and were centrifuged at 3000 rpm for 10 min. The absorbance of the supernatant was measured at 535 nm and evaluated as nM of MDA tissue using the molar extinction coefficient of 1.56 × 10^5^/M cm.

### 2.6.5. Determination of peroxidase activity {#sec0130}

Peroxidase activity was determined by the peroxidase kit method (CAS Number 7722-84-1, Sigma--Aldrich) supplier with modifications. A solution containing the mixture of 1 mL of the oxidant solution (FeCl~3~, 100 mM) and the extract or vitamin C (standard) for a final concentration of 100 μg/mL was incubated for 1 h in a water bath at 37 °C. An aliquot of PBS (0.1 mL), hydrogen peroxide (50 μL), and pyrogallol solution (110 μL) were added to 625 μL of distilled water that was earlier poured into an Eppendorf tube. The plant extract (75 μL) from the mixture was thereafter


because of their prospective health benefits [@bib0470]. Indeed several studies have demonstrated that foods from plant origin having antioxidants are beneficial to health since it down-regulate many degenerative processes and can efficiently lower the rate of cancer and other diseases [@bib0335], [@bib0340], [@bib0470], [@bib0475]. The recovery of antioxidant substances from plant materials is usually realized through different extraction techniques taking into account their chemistry and distribution in the plant matrix. In the present study two solvents were used for the extraction of the leaves and stem of *P. guineense*. The ethanol used for extraction gave a yield of about 11% and 7.46% respectively for the leaves and stem extracts while the hydro-ethanol solvent extraction produced 12.66% and 18.92%. Polar solvents are frequently used for the recovery of phenoliccompounds from a plant matrix. According to this result the hydro-ethanol solvent was more active; corroborating previous studies which stated that the amount of active compounds in extracts is related to the polarity of the solvent and the hydro-alcoholic solvents have been demonstrated to increase the yields [@bib0480]. Several methods have been described in order to assess the antioxidant and the free radical quenching properties of plant materials among them, the DPPH scavenging method which has provided information on a great deal of plants and is widely used. DPPH• is a stable and organic nitrogen radical bearing no similarity to the highly reactive and transient peroxyl radicals involved in various oxidative reactions *in vivo* [@bib0300].

The results of DPPH scavenging potential of the plant extracts are presented in [Fig.


action by the Lafayette Parish Council can repeal or overturn a contract or agreement executed by LEDA that was valid and legal when it was executed.

Present law gives the Lafayette Parish Council oversight responsibility for LEDA and, in what appears to be language unchanged since LEDA was a harbor and terminal district board, requires the board to annually report to Lafayette Parish the arrivals and departures of vessels and tonnage along with imports and exports going through the port district. The proposed bill removes both of those clauses.

LEDA's budget goes before the City-Parish Council, but the council doesn't have oversight of the budget.

Most of LEDA's money — it had a nearly $17 million balance at the end of 2017 — comes from a parish-wide property tax set by“We continue to feel, hype notwithstanding, that this is one of the most important authors to emerge in the last decade, and we’ll try to stay on top of the work yet to appear: an essay collection, a book of poetry, and The Sorrows of the Real Policeman (a.k.a. the ” sixth part of 2666″)”


grassroots who can bring democracy to life beyond rhetoric — are a real potential threat to that power.

Accusations of aid and comfort to the enemy were profuse after Martin Luther King Jr. moved forward to expose the Johnson administration’s deceptions and the U.S. military’s atrocities. Most profoundly, with his courageous stand against the war in Vietnam, King earned his Nobel Peace Prize during the years after he won it in 1964.

Bradley Manning may never win the Nobel Peace Prize, but he surely deserves it. Close to 60,000 people have already signed a petition urging the Norwegian Nobel Committee to award the prize to Manning. To become a signer, click here.

Also, you can preview a kindred project on the “I Am Bradley Manning” site, where a just-released short video— the first stage of a longer film due out soon — features Daniel Ellsberg, Oliver Stone, Maggie Gyllenhaal, Phil Donahue, Alice Walker, Peter Sarsgaard, Wallace Shawn, Russell Brand, Moby, Tom Morello, Michael Ratner, Molly Crabapple, Davey D, Tim DeChristopher, Josh Stieber, Lt. Dan Choi, Hakim Green, Matt Taibbi, Chris Hedges, Allan Nairn, Leslie Cagan, Ahdaf Soueif and Jeff Madrick.

From many walks of life, our messages will become louder and clearer as Bradley Manning’s trial continues. He is guilty of “aiding the enemy” only if the enemy is democracy.

Norman Solomon is co-founder of RootsAction.org and founding director of the Institute for Public Accuracy. His books include “War Made Easy: How Presidents and Pundits Keep Spinning Us to Death.”


THE professor slammed for making an "innocent" lingerie joke has said the backlash he's faced is an attack on free speech.

Richard Ned Lebow was asked which floor he wanted while in a lift and replied "Ladies' lingerie" - leading a colleague to complain.

2 Richard Ned Lebow, 76, faces disciplinary action after he was asked which floor he wanted in a lift and replied: 'Ladies’ lingerie'

The 76-year-old has spoken out and refuses to apologise as he thinks he did nothing wrong.

The ISA deemed that it violated its code of conduct — and has given him until Tuesday to apologise.

However he has so far refused saying his case violates freedom of speech, and he could face disciplinary charges, putting his 53-year career in jeopardy.

The US academic called the complaint “frivolous”,as the substrate.  This deck, which may be composed of a variety of materials, is the structural member which bears the load of the insulation and waterproofing.

7
It is the waterproofing alone which protects both the interior of the building and the substrate itself from water which alights upon the roof.  The substance applied to waterproof the substrate is referred to by its generic name of bitumen, and it may be either a tar or an asphalt.  This bitumen, be it tar or asphalt, lacks the elasticity required to accommodate normal movement of the structural deck, either thermal or moisture induced.  Accordingly, in order to enable the bitumen to resist structural movement and maintain its efficacy as a waterproofing agent, layers or plies of felt


the building was improperly constructed.  Unworkmanlike installation of the roof in two particulars may well account for, and at the least contributed to, the development of the leaks which eventually required that the "Bond Ply" membrane be replaced.  Testimony was adduced which indicated that ponding, which results from improper sloping in the roof, occurred in the relatively flat area between the gymnasium and the domes and in the area of the domes themselves.  Leaking took place in both of these locations.  Upon opening up the roof for inspection, Olson observed felt plies which had deteriorated to the point where a substantial amount of water was entering the school.  This deterioration was attributed to the lack of proper drainage.  McNeil testified that asphaltavailable in the United States, IBOC digital radio may suffer from several shortcomings. For example, there may global standardization problems. Though the United States favors IBOC, the European and Canadian communities seem to favor L-Band making the establishment of a global standard difficult.


Vice President-elect Joe Biden said he received a promise from Obama before he accepted the offer to join the ticket.

(CNN) – Before he accepted Barack Obama's offer to join his presidential ticket, Joe Biden got a promise from Obama: that he would be there for "every critical decision," Biden said in an interview broadcast Sunday.

Speaking to ABC's "This Week," Biden said he believes the vice president's role is to provide "the best, sagest, most accurate, most insightful advice and recommendations he or she can make to a president to help them make some of the very, very important decisions that have to be made."

When Obama talked to him about being his number two, "I said, 'I don't want to be picked unless you're picking me for my judgment.I don't want to be
the guy that goes out and has a specific assignment... I want a commitment from you that in every important decision you'll make, every critical decision,
economic and political, as well as foreign policy, I'll get to be in the room.'"

Biden said President-elect Obama has kept the promise, having Biden in the room for all of his decisions about who will fill key posts in the administration.

In what ABC billed as Biden's first interview as vice president-elect, Biden also discussed the role he played in helping Sen. Hillary Clinton decide to accept Obama's offer of serving as his secretary of state.

"She's one of my close friends. And when this came forward, I did talk to her. She sought me out. I sought her out as


then don't expect too much.

December 21, 2008 04:32 pm at 4:32 pm |

nicholas

A good mix . Will take U.S there..............................

December 21, 2008 04:32 pm at 4:32 pm |

FreeNLovIt

I dont care about what, where, when...... JUST get the JOB done!!!

December 21, 2008 04:41 pm at 4:41 pm |

billy-bayou

"Biden the working famlies Czar"

just what we need, a frigging Czar to keep the obamanation company.

December 21, 2008 04:41 pm at 4:41 pm |

FreeNLovIt

To Dan

We will need to pray. If America doesnt make it, it looks like, we'll have to join the New World Order, EU.

December 21, 2008 04:42 pm at 4:42 pm |

Tom

Who are you kidding, Mike? It IS in the tank.

December 21, 2008 04:48 pm at 4:48 pm |

ironmen1987

Obama could've went to a mental ward and found a more stablethank heavens so many aren't.

Of course Biden will be there for every "critical"decision. Biden is the one with thirty years experience. Obama needs his input.

December 21, 2008 05:16 pm at 5:16 pm |

Arundel

Biden is an idiot...Period

December 21, 2008 05:18 pm at 5:18 pm |

TheAsianAmericanMind.com

We might just have an excellent combination – the inexperienced and the experienced. It does not mean to say it is bad at all for a president to be that, it is just that he has to learn fast while in the hot seat. At least it looks like he has done a very good job looking for a good group of people as his supporting cast. I wish them well.

December 21, 2008 05:24 pm at 5:24 pm |

Donna from Ohio

If Biden is so smart


I need.
use of Organization.IsSandbox ==> not good enough, does not tell me which of the 25+ possible sandboxes it is. Only that's not production. I need more
use of Organization.Name ==> all the same for all sandboxes
use of Organization.InstanceName ==> provides the server name like CS83, very nice, but not good enough

I think there is no officially supported way to get it... but I'm not 100% sure...
Is there any approach to "hack" that sandbox name out of the guts of the platform? I need no sample code, just an idea or a concept is good enough for me.

A:

After Spring'16 release there is an ability to specify an Apex class which executes every time the sandbox is copied. See SandboxPostCopy Interface. You can get the sandbox name from SandboxContextsome overacting, but not as much as one would expect, and even when it does appear it fits in as part of the game’s odd charm.

At the end of the day Knee Deep is a unique and somewhat decent niche game that will only appeal to a certain group of gamers. Those who might be on the fence in regards to purchasing it should definitely do their research and watch some gameplay videos before jumping in, because at $15 USD, it’s an expensive proposition as a blind buy. Hell, even those with interest would be best to wait for a sale, given that the current asking price is a bit on the high side for a three hour-long game with limited replay value.


I had separately given testimony at the hearing held in San Francisco. This new hearing was a good chance to remind the Copyright Office of the First Amendment concerns with the DMCA we had already warned them about, many of which are just as worrying — if not more so — today.

One significant, overarching problem is the way the DMCA results in such severe consequences for speech, speakers, and platforms themselves based on the mere accusation of infringement. It is unique in American law for there to be such an effect like this: in most instances, sanction cannot follow unless and until a court has found there to be actual liability. In fact, when it comes to affecting speech interests it is expressly forbidden by the First Amendment1.Thomas Rawls, RB, Seattle Seahawks

Rawls might be a surprising name to see on this list, as he is the most obvious heir apparent to the now retired Marshawn Lynch in the Seahawks backfield. But his recovery from an ankle injury that ended his brief-but-excellent 2015 campaign and Seattle’s drafting of Notre Dame running back C.J. Prosise have cast some doubt as to whether Rawls will be the lead back. So long as Rawls is healthy, the answer is a clear yes. He earned the fourth-best PFF rushing grade among backs during his limited action last season, and ranked third in yards after contact per attempt – a sign of his ability to produce independent of Seattle’s run-blocking. He is one of 2016’s potential breakout stars so long as


consequence of a miRNA binding to its target gene is the mRNA degradation and/or inhibition of protein translation \[[@B3-ncrna-01-00127],[@B4-ncrna-01-00127],[@B5-ncrna-01-00127]\], although there have been reports of miRNAs stabilizing their target transcripts \[[@B6-ncrna-01-00127],[@B7-ncrna-01-00127]\].

Most miRNAs are encoded by independent transcription units \[[@B8-ncrna-01-00127]\], located in intergenic regions or hosted within introns of protein-coding or non-coding genes \[[@B9-ncrna-01-00127],[@B10-ncrna-01-00127],[@B11-ncrna-01-00127],[@B12-ncrna-01-00127],[@B13-ncrna-01-00127]\]. Beside these intronic miRNAs that are potentially transcribed independently of the host genes, novel classes of intronic miRNAs precursors have been reported recently by which production is initiated by splicing of the intron, independently of the microprocessor \[[@B10-ncrna-01-00127],[@B11-ncrna-01-00127],[@B14-ncrna-01-00127]\]. This class currently consists of mirtrons---thereafter subdivided into canonical, 3′ and 5′ tailed that require exosome-mediated trimming after splicing---and simtrons---that require splicing and Drosha but not DGCR8---inherently co-regulated with their host gene and by alternative splicing ofdown using bait \#2. All the other clones were empty (3/25 and 25/25 from bait \#2 and bait \#3 experiments, respectively). These data perfectly match miRBase data along which the ratio between reads mapping the guide and the star sequences is about 99.5% \[[@B20-ncrna-01-00127],[@B21-ncrna-01-00127],[@B22-ncrna-01-00127]\] while we obtained a ratio of 98% using our method. It is worth mentioning that even though the mature hsa-miR-21 star strand is highly under-represented according to miRBase, our developed psRNA-seq was sensitive enough to detect and clone it from a small number of colonies.

![(**A**) Schematic representation of the 528.922 reads count corresponding to hsa-miR-21 according to miRBase release 21; (**B**) Schematic representation of the result of the 47 plasmids sequenced. Out of the 47 clones, 46 were corresponding to the guide mature miR-21


represents about 1 ng of total RNA and as little as 100 human cells).

4.  psRNA-seq is a simple, rapid, and inexpensive method to set up. It does not require special skills in molecular biology or bioinformatics. Indeed, it does not require long and tricky procedures to prepare libraries to be sequenced by RNA-seq technologies or challenging bioinformatical analysis of large datasets.

5.  psRNA-seq is highly flexible. It is not specific to miRNAs (canonical and from intron origin) and can also be used to capture plenty of other ncRNAs such as snRNAs, snoRNAs and many other still-unknown small RNAs. The method is also easily transposable to other species, with some restrictions (see below).

We should mention some limitations to the technique. First, psRNA-seq relies on the initial knowledge ofwell as piRNAs, possess a 2′-O-methyl modification on the ribose of their 3′ extremity \[[@B31-ncrna-01-00127],[@B32-ncrna-01-00127],[@B33-ncrna-01-00127]\] which prevents the addition of nucleotides to the 3′-end and therefore the activity of the poly(A) polymerase \[[@B32-ncrna-01-00127],[@B33-ncrna-01-00127]\].

In support of the use of psRNA-seq, this method recently allowed us to identify and clone a novel mirtron produced by a human gene in specific developmental conditions (in preparation; to be published elsewhere) and from another pathogenic locus is disease conditions (in preparation; to be published elsewhere).

4. Material and Methods
=======================

Detailed experimental protocol is given in [Supplementary Data 1](#ncrna-01-00127-s001){ref-type="supplementary-material"} and a scheme of the pocket-sized RNA-seq method is shown in [Figure 1](#ncrna-01-00127-f001){ref-type="fig"}.

4.1. Bait Preparation
---------------------

The first step requires the production of *in vitro* transcribed RNA from the genomic region of interest that will be used as bait.


Entertainment

Google Gets Serious About Real Estate Search ... In Australia

Google has been steadily upgrading its Maps, seemingly adding a new feature here and there every couple of weeks. It's not always global, though. For example, they've just added real estate search to Maps in Australia.

It's a sign of the things to come, as this real estate search seems as good (or better) as any you've seen on various real estate sites out there. Go to Google.com.au and type in a search query such as real estate Sydney. You'll get all of the properties in the area, with options to further refine your search to include properties that are for rent or for sale, set a price range, the desired number of bedrooms and bathrooms and so forth.

Of course,M sodium cacodylate buffer (pH 7.2) at 4°C for 2 hrs, post-fixed in 1% osmium tetroxide (Canemco-Marivac) for 2 hrs and placed in 0.25% uranyl acetate (Canemco-Marivac) at 4°C overnight. Tissue specimens were dehydrated in graded series of acetone and embedded in Epon Araldite resin (Canemco-Marivac) and polymerized for 48 hrs at 60°C. Ultrathin sections (∼80 nm) were cut using an ultramicrotome (LKB Huxley, England), placed on copper grids and stained with uranyl acetate and lead citrate. Samples were examined using a JEOL JEM 1230 transmission electron microscope (TEM) and images were captured using a Hamamatsu camera.

Immunogold labeling technique
-----------------------------

Ventricular specimens were fixed overnight with 4% paraformaldehyde and 0.5% glutaraldehyde in 0.1 M sodium cacodylate buffer and dehydrated in a graded series of ethanol. After embedding in LR White


frequently found in close proximity to the endocardial cell layer. Many of these undifferentiated cells formed cell to cell junctions with adjacent undifferentiated or moderately differentiated and mature myocardial cells via spot or belt desmosomes ([Fig. 1D](#fig01){ref-type="fig"}).

![Ultrastructure analysis of E 11.5 ventricular myocardium. (**A**) Two adjacent undifferentiated cells with sparse cytoplasm: note the presence of belt desmsomes between these two cells (arrows), Scale bar = 2 μm. (**B**) A moderately differentiated myocardial cell with loosely arranged myofilaments (arrowhead) and a primitive sarcomere (arrow) in the cytoplasm, Scale bar = 2 μm. (**C**) A mature myocardial cell containing myofilament bundles with well-defined sarcomeres (arrow-heads), Scale bar = 500 nm. (**D**) A belt desmosome (arrow) between two undifferentiated cells, Scale bar = 100 nm. (**E**) A spot desmosome (arrow) betweencontained several myofilament bundles arranged into well-organized sarcomeric structures, abundant mitochondria, secretory granules as well as other organelles ([Fig. 1C](#fig01){ref-type="fig"}). Both moderately differentiated and mature myocardial cell types formed cell to cell junctions with each other mainly via desmosomes and fascia adherens ([Fig. 1E](#fig01){ref-type="fig"}). Intercalated discs containing gap junctions, desmosomes and fascia adherens were also observed in many of the differentiated cells ([Fig. 1F](#fig01){ref-type="fig"}).

Quantitation of undifferentiated and mature myocardial cells in the developing ventricular myocardium
-----------------------------------------------------------------------------------------------------

Based on the ultrastructural characteristics described earlier, we assessed the percentages of undifferentiated, moderately differentiated and differentiated cells in the E11.5 ventricular myocardium ([Fig. 2](#fig02){ref-type="fig"}). We defined myocytes as moderately differentiated cells when the cytoplasm contained scattered myofilaments lacking organized sarcomeric structures. At E11.5, we found that the majority of ventricular myocardial cells were


         32 ± 0.4%^\^\^^                             61.0 ± 0.7%

Data is presented as mean ± S.E.M., *N*= A total of 600--1000 cells from three to five hearts per group.

p \< 0.05 E11.5 UC *versus* E16.5 UC and p \< 0.005 E11.5 UC *versus* E18.5 UC.

[\*](#tf1-2){ref-type="table-fn"}p \< 0.005 E11.5 MC *versus* E11.5 UC and E11.5 MDC.

\#p \< 0.05 E11.5 MC *versus* E16.5 MC and p \< 0.005 E11.5 MC *versus* E18.5 MC.

\^\<0.05 E16.5 UC *versus* E16.5 MDC.

\<0.05 E18.5 UC *versus* E18.5 MDC and p \< 0.005 E18.5 UC *versus* E18.5 MC.

\<0.005 E18.5 MDC *versus* E18.5 MC.

Immunocharacterizationcardiac abnormalities and associated embryonic deaths seen in several genetic models in which formation of a chambered heart is unperturbed or only modestly affected \[[@b32]\]. Our findings from this study suggest that multiple mechanisms may regulate further maturation of undifferentiated and moderately differentiated myocardial cells in later stages of cardiac development. We propose that any imbalances in regulatory mechanisms that can alter the ratio of undifferentiated cells to differentiated myocytes could result in late embryonic or postnatal mortalities reported for several genetic models \[[@b32]\]. For instance, retinoids were shown to play a key role in the early stages of cardiac development to prevent cell differentiation and favour cell proliferation \[[@b37]\]. Ablation of retinoid signaling pathway was shown to result in precocious myocardial cell maturation and early embryonic lethality


20 percent are related to our [individual] business,” he says. Enter the Startup Act. Recently voted into law, it is designed to reward entrepreneurship and provide administrative help at every stage of a startup’s life cycle. And then there’s the funding issue.

Although the GEI gives risk capital a 70 percent score, Saidane questions this assessment. It takes up to 18 months to secure funds, she says, which is “long enough to kill any business.” That said, she is on the steering committee developing a new Central Bank–supported Fund of Funds that would channel monies from big players like the African Development Bank into various smaller funds. Designed to provide incentives for new venture capitalists to come to market, Saidane hopes it will take off by mid-2019. As forSNP. This ensures that SNPs with poor discrimination are treated as uninformative in the next step of the analysis.

Wavelet transform {#s6}
-----------------

The resulting SNP-level admixture signals indicate how ancestry varies along the genome, but they invariably exhibit a high noise-to-information ratio. To interpret the signal, its frequency content can be described using the wavelet variance ([@bib26]). The wavelet variance $S_{j}~$ for scales $j = 1,\ldots,J$ provides a scale-by-scale decomposition of the variance of the signal. The first scale ($j = 1$) captures the highest frequency patterns, representing very local information. Increasing the scale index provides successively coarser, or lower frequency information, equivalent to "zooming out" on the signal until the level of the entire chromosome is reached. A plot of $S_{j}$ *vs.* *j* indicates which scales are important contributors


to the process variance and indirectly provides information about the distribution of admixture tracts. For example, recent admixture produces a peak in the wavelet variance at a large wavelet scale, reflecting long admixture tracts, while more ancient admixture events produce peaks at lower wavelet scales, reflecting shorter admixture tracts.

The wavelet variance for an individual *i* is given by$$\left. S_{j}^{i} = \frac{1}{T}\sum\limits_{k = 1}^{T} \middle| d_{j,k}^{i} \middle| {}_{2}, \right.$$where $d_{j,k}^{i} = {\sum_{s}Y_{s}^{i}}\psi_{j,s - k}$ are the wavelet coefficients for the signal $Y^{i}$ constructed using the wavelet system $~\psi$. To appreciate the methodology, it is sufficient to understand that the wavelet variance reflects the frequency content of the signal, but more detailed background material is provided in the [Supporting Information](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-11.pdf) ([Figure S5](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-5.pdf), [Figure S6](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-10.pdf), [Figure S7](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-2.pdf), [Figure S8](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-9.pdf), [Figure S9](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-3.pdf),and [File S1](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-4.pdf)). Our implementation employs Daubechies' least asymmetric wavelet number 8 in the *waveslim* ([@bib37]) package of the statistical software R ([@bib32]). We emphasize, however, that the methods proposed here are robust to other choices of analyzing wavelet (see [Supporting Information](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-11.pdf), [Figure S5](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-5.pdf), [Figure S6](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-10.pdf), [Figure S7](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-2.pdf), [Figure S8](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-9.pdf), [Figure S9](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-3.pdf), and [File S1](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-4.pdf)).

Population averages are computed as $$S_{j}^{\text{C}} = \frac{1}{n_{\text{C}}}\sum\limits_{i \in P_{\text{C}}}S_{j}^{i}$$(and similarly for populations *P*~A~ and *P*~B~). An example of the average wavelet variance for each population is shown in [Figure 1B](#fig1){ref-type="fig"}. The wavelet variance is highest at fine scales, but as the ancestral populations also show this pattern, it should be considered background noise. It is intuitive that the very finest wavelet scales are uninformative because small numbers of SNPs should be insufficient to


and 0.025 (ancestry predominately from *P*~A~). For this analysis, the time of admixture was fixed at 160 generations. As the proportion of admixture decreases, the raw wavelet variance exhibits increasing levels of noise relative to informative variation. This is shown by the reduced magnitude of the informative wavelet variance ([Figure 3](#fig3){ref-type="fig"}) and emphasizes that, as expected, it is increasingly difficult to extract informative variation at low admixture proportions (small *p*) even where the signal is technically present. In this example, informative estimates were obtained for admixture proportions as low as 2.5%, although in general, the range of *p* for which this method is applicable will also depend on other characteristics of the data, such as the SNP density and sample size, as considered in the next section.

![Relationship betweenABS metrics observed under the standard reference condition. (A) Potential sources of error (conditions 2--5); (B) varying SNP densities (conditions 6--8). Note that the decline in absolute values of the ABS metrics in B is expected; these are easily accounted for in an inference setting because the SNP density is always a known variable. Condition descriptions and numeric values are presented in [Table 2](#t2){ref-type="table"}.](469fig4){#fig4}

Condition 1 shows the original results, exactly as described above. New simulations were then performed to mimic realistic linkage disequilibrium (LD) (condition 2). To do so as accurately as possible, we applied the real recombination rates observed along the first 100 Mb of chromosome 1, as recombination rates for chromosome 1 are near the average of all chromosome-level recombination rates ([Figure S2](http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.176842/-/DC1/genetics.115.176842-1.pdf)). The effect of


to distinguishing local features in data and could be helpful in this regard, identifying features that may not be easily detected by considering the localized admixture signals in their raw form.

Other prospective areas for further work include the extension of these methods to the more general case of multipopulation admixture. [@bib20] describe the use of PCA as a diagnostic in this setting, and PCA has been used to assign multipopulation ancestry in the software *PCAdmix* ([@bib4]). The wavelet methods described here could be extended in a similar way by considering pairwise combinations of any number of ancestral populations.

In contrast, key restrictions that determine our ability to reconstruct admixture events include the degree of differentiation between the ancestral populations and the representativeness of samples used as surrogate ancestral groups.we aim to describe between-population variation, while ignoring within-population variation. This is achieved by selecting the first principal component, as long as the sample sizes are sufficiently large. Within-population fluctuations of individual coordinates on the PCA scatterplot can be caused by subtle population substructure. Assuming that no such substructure is present, these fluctuations decrease as the total sample size increases, and an asymptotically stable pattern of the eigenvector plot results ([@bib19]). When the number of individuals is large, variation between individuals from the same population is small compared to that of the different populations, so that the first eigenvector describes the primary population structure of the data. However, as the sample size decreases, individual variation carries more weight, which may be addressed in more than the first principal


Aspects of Reaction on Solid Supports, Stark, ed. Academy Press, pp. 189-213, 1971.
It is an object of the present invention to provide a novel form of entrapment of the functional material which does not encapsulate the functional material.
This invention provides a unique combination of polymers and functional materials, which results in compositions wherein the functional materials rather than being encapsulated by coating materials are dispensed throughout and entrapped within a hydrophobic polymeric lattice. These compositions are useful for incorporating a variety of functional materials into a variety of products such as cosmetic and non-cosmetic products. Furthermore, the amount of functional materials which can be entrapped in the lattice are much higher than heretofore achievable by encapsulation.All relevant data are within the paper.

Introduction {#sec001}
============

Hepatitis C virus (HCV), a member of the genus *Hepacivirus* within the virus family *Flaviviridae*, is able to establish chronic infection in humans, eventually leading to liver cirrhosis, hepatocellular carcinoma and liver failure \[[@pone.0117514.ref001],[@pone.0117514.ref002]\]. More than 170 million people worldwide are infected with HCV, 80--85% of which are chronic infections. Annually, 3--4 million people are newly infected and up to 350,000 people die from HCV related liver diseases every year (World Health Organization, [www.who.int](http://www.who.int)). HCV vaccine development is hampered by viral genetic heterogeneity conferring immune evasion and the lack of relevant and practical animal models \[[@pone.0117514.ref001],[@pone.0117514.ref003]\]. The standard treatment for hepatitis C uses pegylated interferon-α (PEG-IFN) combined with ribavirin. Dependent on HCV genotype, this treatment is successful in 45--80% of cases.


\[[@pone.0117514.ref014]--[@pone.0117514.ref017]\].

In the present study, we evaluated the effects of AnA on the HCV life cycle. We demonstrated that AnA inhibits HCV entry, replication, translation, and virion secretion in a dose-dependent manner. Furthermore, inhibition of histone acetyltransferase activities by two chemical inhibitors and RNAi suppressed HCV replication, suggesting a possible mechanism by which AnA exerts its inhibitory effect on HCV.

Material and Methods {#sec002}
====================

Plasmids, inhibitors and antibodies {#sec003}
-----------------------------------

HCV genomic plasmids HCV-2a J6/JFH-1 and HCV-2a J6/JFH-1(p7-rLuc2A) harboring *renilla* luciferase (rLuc) reporter were received from Dr. Charles Rice \[[@pone.0117514.ref018]\]. Lentivirus production plasmid psPAX2 was obtained from Dr. Didier Trono as Addgene Plasmid 12260. HCV RNA translation reporter plasmid pHCV16LUC and lentivirus vector based RNAi plasmid for p300/CBP-associated factor (PCAF) were kindly provided by Drs. Matthias Gromeier, Shelton Bradrick, and Qiwei Zhai, respectivelyprotein concentration quantified by Bradford assay according to the manufacturer's protocol (Bio-Rad).

Western blot {#sec009}
------------

Protein samples in Laemmli buffer were subjected to SDS-PAGE and transferred to nitrocellulose membranes. The blots were blocked in 5% skim milk in PBS for 1 h and incubated with primary antibody (1:1000 in PBS/0.1% Tween-20) overnight at 4°C. After washing with PBS, the blots were incubated with an infrared dye-labeled secondary antibody (1:10000, Li-Cor Biosciences) for 1 h at room temperature and then washed again. The blot was scanned using an Odyssey Infrared Imaging System (Li-Cor Biosciences) and fluorescent intensity of protein bands was quantified using the Odyssey software.

HCV pseudoparticle (HCVpp) entry assay {#sec010}
--------------------------------------

HCV lentiviral pseudoparticles carrying luciferase reporter gene (HCVpp-Luc) were produced in HEK293T cells by co-transfecting psPAX2, pTRIP-CMV-Luc and pcDNA3.1(+)-HCV-2a J6 core-E1--E2


SUMOylation of cellular proteins by AnA has an effect on HCV life cycle.

The exact mechanisms behind HCV assembly and secretion are poorly understood. Data collected so far suggest that HCV assembly and secretion is closely linked to host very-low-density-lipoprotein (VLDL) and LDL pathway \[[@pone.0117514.ref030],[@pone.0117514.ref045]\]. It is conceivable that AnA may interfere with this pathway.

In conclusion, we showed that AnA can inhibit HCV entry, replication, translation, and secretion in a dose-dependent manner with no measurable effects on cell viability. Our data suggest that at least part of the anti-HCV activity is through inhibition of HAT activities. Further elucidation of the molecular mechanism by which AnA inhibits different phases of HCV life cycle in future studies will provide attractive drug candidates for hepatitis C.

We thank Drs. Charles Rice, Stan Lemon,President Trump, his elder children and his private businesses are suing Deutsche Bank and Capital One over congressional subpoenas issued to the organization, court papers filed Monday show.

Details: The lawsuit, first reported by the New York Times, was filed in the Southern District of New York. The move is in response to subpoenas from the House Financial Services Committee and the Intelligence Committee seeking to obtain Trump's financial records.

The big picture: The Trump administration has taken several steps to block oversight by House Democrats in recent days. Trump says he's against current and former White House aides testifying before congressional panels. Following the release of the Mueller report, Trump is turning to litigation strategies that he long used in business — resist, delay and sue, per Axios' Mike


Allen.

What they're saying: In the lawsuit, Trump's lawyers argue the subpoenas were issued to harass him: "to rummage through every aspect of his personal finances, his businesses, and the private information of the President and his family, and to ferret about for any material that might be used to cause him political damage. No grounds exist to establish any purpose other than a political one," it says.

The other side: Financial Services Committee chair Rep. Maxine Waters (D-Calif.) and Intelligence Committee chair Rep. Adam Schiff (D-Calif.) said in a joint statement to media outlets that the lawsuit is a "meritless" demonstration of the "depths to which President Trump will go to obstruct Congress’s constitutional oversight authority."

"As a private businessman, Trump routinely used his well-known litigiousness and the threat ofQ:

Boolean in view is true but in controller false angularJS

In parent scope (external one which wrap entire webapp) I define boolean variable if you're logged in.
$localForage.getItem('authorization')
    .then(function(authData) {
            if(authData) {
                $scope.authentication.isAuth = true;
                //token is added in http interceptor
            } else {
                $scope.authentication.isAuth = false;
            }


}, function(){
            console.log("error with getting authorization localForage after refresh");
        }
    );

It is basically working correct. Now I can build UI basing on this boolean with ng-if="$parent.authentication.isAuth". I also can display true/false like so
{{$parent.authentication.isAuth}}
 in view and it is also working correct.
In one controller I want to use this boolean inside controller not in view. So I do if($scope.authentication.isAuth){ or if($scope.$parent.authentication.isAuth){ (I tried both) and this condition go to else even when
{{$parent.authentication.isAuth}}
 in view of controller where I'm using this condition display true.
So it

{{$parent.authentication.isAuth}}

display true in paragraph and within controller named ctrl this conditiongame off a little slow. As the game went on in the first half, they picked up their level of intensity on the defensive end and took us out of everything we tried to run offensively. We were very fortunate to get some baskets in transition."

[on outrebounding the opponent at home again]
"I'm not sure why that is. I think we play a lot more aggressive at home, and play all around better at home. I'm sure if you look at our numbers, they're higher in every category at home."

[on Greg Collucci's minimal performance]
"Yesterday in practice, he took a jump shot in the corner and hurt his ankle. We weren't even sure if he'd be able to play at all today. He got treatment all last night...Fortunately for us,


Marquin [Chandler] was able to step up and fill the void."

[on Jaason Smith's perseverence in the latter stages of the game]
"I think that says a lot about his character. He has a tremendous will to win. At the end, he was going to do whatever it took to help us win the game. The last rebound he got--the one where there was a travel called on the play--that was as big a rebound as I've ever seen. I don't know where he came from. All I know is he was going to get that rebound no matter what. If there was a Mack truck out there, he was going to get that rebound."

[on Marquin Chandler]
"He adds another terrific offensive player. He adds more size and versatility to ourWe started this project in May. This is a two unit home and we are remodeling the entire top floor unit. The clients need functional, flexible space for a young family of four. To achieve this we will remove all of the dividing walls in the public space of the house and relocate the kitchen. We will also add a new full mater bath and closet to the master bedroom and a laundry room to the guest/kid's bathroom. More windows and skylights will bring in even more beautiful natural light, which will be carried down into the ground floor unit since we are removing the wall around the back stairs.

And just for fun here are some alternate layout options we played around with (note the third bathroom we


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Quantum Code and “The Wall Street Wizard”

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