[1](#Tab1){ref-type="table"}, and the carrier females lens opacity and vision were unavailable here.Fig. 1Main clinical manifestations of the proband. **a** Slit lamp photograph showing that the proband presented with bilateral congenital cataracts; **b** The screwdriver blade-shaped incisors; **c** One missing maxillary second molar; **d** The mild mulberry-like molarsTable 1Manifestations of the available family membersIDGenderAgeCataractNystagmusDental anomaliesI-1Male60NNNI-2Female60NNNII-1Female36NNNII-2Female34NNNII-3Female32NNNII-4Female30NNNII-5Male28YYYII-6Male26NNNIII-1 (Proband)Male15YNY*Y* Yes, *N* No

The proband (III:1) and another affected individual (II:5) underwent microincision cataract surgery at the ages of 15 and 21 years respectively. Available photos before the surgery revealed bilateral lens opacities that heavily affected the nucleus in the proband (Fig. [1](#Fig1){ref-type="fig"}; Fig. [2](#Fig2){ref-type="fig"}). Individual II: 5 presented with similar facial features including a long narrow face, mildly anteverted pinnae ear and screw-driver like incisors, mild mulberry like molars and malocclusion (Additional file [1](#MOESM1){ref-type="media"}).those reported in ClinVar and Cat Map databases were reviewed in the table. (XLSX 14 kb)

CADD

:   Combined annotation dependent depletion

Chr

:   Chromosome

EDTA

:   Ethylene diamine tetraacetic acid

ExAc

:   The exome aggregation consortium

GATK

:   Genome analysis toolkit

InDel

:   Insertions and deletions

NHS

:   Nance-Horan syndrome

*NHS*

:   *NHS* gene

OMIM

:   Online Mendelian inheritance in man

PolyPhen

:   Polymorphism phenotyping

SIFT

:   Sorting intolerant from tolerant

WES

:   Whole exome sequencing

We thank the patient and the families for participating in the genetic study, donating blood samples and providing detailed medical history.

Funding {#FPar1}
=======

This study was supported by the Fundamental Research Funds of the Chinese Academy of Medical Sciences. The funding number is: 2016RC310006.

Availability of data and materials {#FPar2}
==================================

The whole exome sequencing data were


you know, most people use the same avatar on multiple Web sites, you know, so I just tracked BurnTheWitches1692 to another site with more personal details," "Amy Barrett." "Internet is the devil, man." "And, apparently, so is Miss Barrett." "Principal says she's taking an after-school driver's ed class." "Oh!" "Satan takes many forms, my friend." "Hey, listen, uh, I was gonna tell you about Reeves coming in to NCIS." "Sorry if that was kind of a surprise." "Okay." "I just, you know, I know what it's like." "Two alpha men trying to prove their manhood, you know?" "You see right through me, Tim." "No, I understand." "I mean, I went through the same thing with Tony, you know." "Right." "Yeah." "Oh, here comes our car." "Crap." "Fuzz." "What?""Hey, no fair!" "Take your foot off the brake!" "Take your foot off the pedal." "You okay, sir?" "I stare death in the face every day." "Amy, hands on the wheel." "Ten and two, please." "Okay, well, heard about the high-speed getaway." "Uh, what was the next part of the plan, take Big Ed hostage?" "My daughter's very sorry." "She just wasn't thinking." "What about the threats to Congresswoman Flemming?" "Were you thinking then, Amy?" "Wait, what threats?" "What did you do?" "You were politically active when you were my age." "I peacefully protested animal cruelty in the meat industry." "Hmm." "Amy Lynn Barrett!" "I-I didn't do that." "Oh..." ""The country is going to hell" ""in a handbasket." ""A basket carried by gutless rats" ""fresh off the boat


read the case file." "Um, so Clayton just stopped by to say hi, so I was giving him a rundown." "Oh, I thought that's why you called me." "I didn't mean to step on any toes, pal." "Keep your shirt on." "A shirt you clearly slept in." "Oh, that's because it's so soft." "Is that the same suit as yesterday?" "Okay, so, moving on." "These rats should be covered in an American flag." "Why?" "Because they're Navy rats." "Officers or enlisted?" "All their internal organs are missing, but it appears they died of natural causes." "Blood work shows very high levels of a protein called IL-33." "What is that?" "It's a protein that's being lab tested in the treatment of Alzheimer's patients." "At a Navy lab?" "The onlyIt is now illegal for adults in Canada to have sex with a partner under the age of 16, one of the new provisions of the Tories' violent crime law that came into effect on Thursday.

The Tackling Violent Crime Act raises the legal age of sexual consent in Canada to 16 from 14, the first time it has been raised since 1892.

But the law includes a "close-in-age exception," meaning 14- and 15-year-olds can have sex with someone who is less than five years older.

The Tories said they raised the age, in part, to deal with internet predators. The new law puts Canada's age of consent in line with those in Britain, Australia and most of the United States.

Gun crime punishment increased

The act also includes higher mandatory prison sentences


of the world from one point, as a continuity. Of the three systems of thought--animistic, religious and scientific--animism is perhaps the most consistent and the most exhaustive, the one which explains the world in its entirety.amount of a polymer capable of gelling in the presence of a cross-linking agent so that after the composition has penetrated into an underground formation and positioned itself preferentially in the highly permeable strata, the delayed gelation is triggered by in situ hydrolysis of an ester which reduces the pH of the composition to the gelable range thereby producing in-depth plugging of the strata with the gelled polymer.
U.S. Pat. No. 4,417,623 to Anthony describes a method for consolidating sand with organic silicate wherein unconsolidated sand-like material in a subsurface formation adjacent a borehole of a water, oil, or gas well is consolidated by treating the formation first with a solution of alcohol and organic silicate and then with water. The water causes the organic silicate to hydrolyze and


content is being changed to suit the mindset of a certain section of the majority religion.

Today, she is as determined as ever to fight for the right to free space, dialogue, debate and even dissent. “Quitting is not an option,” she says with confidence, but adds, “The younger lot has to take over. It is their life; their generation should be concerned. It is the next generation and the generation after that which will be impacted enormously. On a broader scale one has to shake that generation. Ask them, ‘Why are you silent?’ They feel if they have a job, everything is all right. That attitude has to go. At another level, one has to fight on, believing that it [state’s repression] is not going to last.”

She answeredgenome.

Members of all the transporter classes are detected as expressed in the mycorrhizal transcriptome (Table [1](#T1){ref-type="table"}). The *L. bicolor*genome encodes fewer transporters than does aspen in the selected analysis set but a larger proportion of the transporter genes is expressed. The expression patterns for the nitrogen and carbohydrate transporters in this study are similar to those reported using whole genome expression arrays \[[@B19],[@B21],[@B22]\]. A single inorganic phosphate transporter is annotated in the *L. bicolor*genome consistent with the previous observations of a limited number of fungal phosphate transporters in mycorrhizal symbiosis \[[@B23]-[@B25]\]. The sets of expressed mycorrhizal transporter genes were analyzed for significant enrichment of specific annotations. At a stringency of Cumulative Binomial Distribution (CBD) pVal \< 0.05, expressed transporters are enriched for amino acid transport in *L. bicolor*and


                                             Aspen          13           53.80%      0.519

Transporters are categorized as \"Nitrogen\", \"Carbon\", and \"Phosphorus\" using GO annotation associated with the indicated metabolic compounds transported in the mycorrhizal metabolome. \"\# Genomic\" indicates the total number of gene models with a specific GO annotation in the aspen or *L. bicolor*genome. \"Expressed\" is the percent of expressed vs. total transporter genes for the indicated organismactivities between aspen and *L. bicolor*. Calcium has been demonstrated to be an important intracellular signal involved in the regulation of fungal-plant symbiosis gene expression \[[@B16],[@B18]\]. Regulation of calcium levels is an early signaling event in many plant/microbe interactions and sequential elevations of cytoplasmic and nuclear calcium levels may have a regulatory role in symbiosis \[[@B34]\]. The phosphatidylinositol signaling system is related to plant development and regulation, such as root hair growth \[[@B35]\]. This mechanism is relevant to ectomycorrhizal interactions, in which plant root hair growth is specifically suppressed in the formation of mycorrhizae by *L. bicolor*. The enrichment of activities for these common signaling pathways is consistent with commonalities observed for modulation of host cell responses in symbioses \[[@B36],[@B37]\].

######

Metabolic pathways significantly enriched for enzyme activities expressed


return all the possible alignments for that sequence read which is required by \'Bowstrap\'. \'BowStrap\' reads the Bowtie output, and for some selected number of iterations, calculates an RPKM value for each gene model. At each iteration, if a RNA seq read was aligned to more than one possible location, that read is randomly assigned to one of those potential locations. From the set of RPKM values calculated across all Bowstrap iterations, an averages and standard deviations of RPKM are determined for each gene model. A statistical measurement to determine if expression of a gene models has been detected at significant levels is calculated as a Cumulative Normal Distribution (CND) and expressed as a pValue (**Eq. 1**)

Where μ is the average and σ is the standard deviation ofread set to gene models identifies a total of 28 (0.0001%) aligned reads. Inspection of the aligned synthetic reads indicated that they are comprised of 2 or 3-mer repeats. Alignment with the synthetic read set demonstrates that observed alignments are highly specific and miss-alignments are not expected.

In our transcriptomic data, at a CND-pValue \< 0.001, for aspen there are 27318 expressed gene models out of a total possible 45555 (60.0%) gene models. For *L. bicolor*, there are 12856 significantly expressed gene models out of 26014 (62.4%).

Gene Model Annotation
---------------------

Annotation to gene model predicted proteins was provided by JGI publicly available downloads using the following files: \"Lacc.ecAnnot.txt\" for (EC) annotation for *L. bicolor*best gene models, \"Lbicolor_GO.txt\" for Gene Ontology (GO) annotation for *L. bicolor*best gene models, \"mntPop.ec.Annotation.txt\" for Enzyme Commission


"use strict";

Object.defineProperty(exports, "__esModule", {
  value: true
});
exports["default"] = void 0;
var ListBoxRole = {
  relatedConcepts: [{
    module: 'ARIA',
    concept: {
      name: 'listbox'
    }
  }, {
    module: 'HTML',
    concept: {
      name: 'datalist'
    }
  }, {
    module: 'HTML',
    concept: {
      name: 'select'
    }
  }],
  type: 'widget'
};
var _default = ListBoxRole;
exports["default"] = _default;We further validated our technique using DNA fragmentation and endonuclease assays. Our results demonstrated that chloroquine pretreatment can significantly enhance accumulation of apoptotic cells in organisms, and we envision combining this method with modern imaging techniques to optimize in vivo detection of apoptosis.


melanin. Although melanoma accounts for only about 4% of all skin
cancer cases, it causes most skin cancer-related deaths. The good news is
that melanoma is often curable if it is detected and treated in its early
stages. Basal Cell Carcinoma is a form of skin cancer. Basal cell carcinomas
are the result of sun damage to the skin. Basal cell carcinomas enlarge
slowly and steadily and can invade neighboring tissue, like the eye, but
they usually do not spread to distant parts of the body (metastasize). The
only way to tell for sure if a skin growth is cancerous is to biopsy it.
Squamous Cell Carcinoma of the skin is a form of skin cancer which is
usually the result of long term sun damage to the skin. West LA dermatology
Board Certified Dermatologist LA dermatology Squamousthe Chinese Google, is accepting payments for its firewall service in the currency.

But there's also suspicion that a large measure of the bitcoin's Chinese popularity is the result of fringe-legal uses. The currency is perfect for getting around the country's tight capital controls, which prevent rich Chinese citizens from moving too much money overseas.

While bitcoin remains unregulated, it is easy for users to buy a large sum in Chinese yuan and sell it in US dollars, evading those regulations.

The potential of bitcoin in China is seen as a large part of the reason for the currency's seven-fold increase in price over November, and the news that the Chinese central bank is taking a less-than-welcoming stance to it has sent markets tumbling.

The value of one bitcoin fell by 28%


important to recognize that the European currency is a political project as much as it is an economic one. European elites welcomed the external constraint of the European Monetary Union (EMU) as a way of depoliticizing economic policy, removing macroeconomic policies from democratic and parliamentary control through a self-imposed reduction of national sovereignty. Their aim was not simply to insulate economic policies from popular-democratic challenges, but also to reduce the political costs of the neoliberal transition by putting the responsibility for unpopular measures onto external institutions and factors. Guido Carli, Italy’s highly influential treasury minister from 1989 to 1992, noted that “the European Union represented an alternative path for the solution of problems which we were not managing to handle through the normal channels of government and parliament.”in whose currency the obligation is expressed. Only about 2.5 percent of Italy’s public debt was issued under foreign law and would have to remain in euros. The rest of the debt was issued under domestic law and could thus be redenominated into the new currency. Nonetheless, a secret and sudden redenomination of all euro-denominated bonds into the new national currency would likely trigger a massive sell-off of government debt, generating a large depreciation of the new currency and inflationary pressures within Italy. For this reason, several economists have suggested ways of mitigating the adverse impact of redenomination. For instance, Giovanni Siciliano, head of research at the Italian securities market regulator CONSOB, writes in his recent book Vivere e morire di euro (Living and Dying by the Euro)


amounts of proximal BCR signaling in these B cells commensurate with their reduced sBCR levels. Functionally, however, they are comparable to nonautoreactive B cells in cell cycle progression, up-regulation of activation and costimulatory molecules, and Ab-forming cell differentiation when treated with a variety of stimuli in vitro. In addition, these B cells can efficiently process and present Ag and are capable of undergoing cognate interaction with naive TCR-transgenic T cells, resulting in robust IL-2 production. Together, these data reveal a lack of intrinsic anergy involving any known mechanism, supporting the idea that this type of antinuclear Ag B cell becomes indifferent to cognate autoantigen by down-regulating sBCR.10. The OS, which came out in 2015, touts enhanced features like Device Guard- which keeps your co…

The dark web may seem like a word you should only say in the dark of night, behind closed doors, but is it really that bad? Yes and no. To some, the dark web is a bad place beyond the internet, a flea market for criminals if you will. Instead of finding handmade jewelry and dusty vinyl records, you'll find illegal drugs, social security cards, and counterfeit money for sale, usually at a relatively low price.¹ Surprisingly, not everyone on the dark web is a malicious criminal. Sometimes, the dark web is a person's only access to the outside world.² People who live in a closed society like North Korea, whose


Q:

How to select multiple JCheckBoxe into ButtonGroup?

I have three JCheckBox like following:
final JCheckBox c1 = new JCheckBox("A");
final JCheckBox c2 = new JCheckBox("B");
final JCheckBox c3 = new JCheckBox("C");

I make a group by ButtonGroup for this checkboxes like following:
final ButtonGroup bg = new ButtonGroup();
bg.add(c1);
bg.add(c2);
bg.add(c3);

I have a Button to display selected items into a label like following:
String SelectedItem="";
Enumeration<AbstractButton> items= bg.getElements();
while (items.hasMoreElements()) {
    AbstractButton btn = items.nextElement();
if(btn.isSelected())
{
        SelectedItem+=btn.getText()+",";
    }
 }
 lblA.setText(SelectedItem);

this work fine , but i cann't select multiple check boxes in run time.

A:

The purpose of ButtonGroup is multiple-exclusive selection. Do not create ButtonGroup only if you want to have a collection of your buttons. Instead of ButtonGroup use a standard collection like ArrayList.
List<JCheckBox> buttons = new ArrayList<>();
buttons.add(c1);
buttons.add(c2);
buttons.add(c3);

...

forneck and arm, and a welt mark from a slotted cooking spoon across one of her cheeks.

The district court was told the baby’s mother had initially lied to police and tried covering up her crime by telling responding officers that her daughter had sustained her injuries in a fall at a playground.

Photos uploaded to Facebook sometime after the attack show the 32-week-old baby with a black eye, abrasions and a small cut on her face, and the welt mark from the slotted spoon on her cheek.

The officers conveyed both Norman and her daughter to the local hospital where a thorough physical examination confirmed Evie had been assaulted and hit in the face and body at least eight times.

In sentencing Norman in the Adelaide District Court last week, Judge


while drunk or distracted. But Americans still need to wake up to the dangers of getting behind the wheel when sleepy, according to a recent study of crash rates. A report released Tuesday by the AAA Foundation for Traffic Safety suggests that drivers who sleep only five or six hours in a 24-hour period are twice as likely to crash as drivers who get seven hours of sleep or more. And the less sleep the person behind the wheel gets, the higher the crash rate, according to the findings. For instance, drivers in the study who got only four or five hours of shut-eye had four times the crash rate — close to what's seen among drunken drivers. "If you have not slept seven or more hours inin a fiery explosion and groundwater contamination . Opponents said the project would result in increased oil and coal train traffic through the Columbia Gorge. Wasco County commissioners voted the proposal down on Wednesday, rejecting the local planning commissions approval of the project. Columbia Riverkeeper attorney Lauren Goldberg said county commissioners were swayed by opposition from local people, including members of the Yakama Nation Indian tribe and Friends of the Columbia River Gorge. Wasco Countys decision is just one example where we see local communities that are respecting the very important input of tribal nations as well as community members, Goldberg said. I think well see more of this. She said even before theOil Train Opponents Hail Track Expansion Defeat In Columbia Gorgehttp://kuow.org/post/oil-train-opponents-hail-track-expansion-defeat-columbia-gorge
92021 as http://kuow.orgFri, 04 Nov 2016


huge jump of 10.4 percent over the same time period in 2015, and it's part of a disturbing trend, according to federal officials, because traffic fatalities rose significantly last year, too. "After decades of progress reducing fatalities, all of a sudden, we're suddenly losing ground," says Mark Rosekind, administrator of the National Highway Traffic Safety Administration. So the government is teaming up with safety advocates, highway designers and others in a new effort to try to reduce the number of traffic fatalities — to zero — within 30 years. "We have an immediate crisis on our hands and we also have a long-term challenge," Rosekind says. The tragic toll is seen everyHuman Errors Drive Growing Death Toll In Auto Crasheshttp://kuow.org/post/tech-human-errors-drive-growing-death-toll-auto-crashes
90805 as http://kuow.orgThu, 20 Oct 2016 08:28:00 +0000Human ErrorsDelicious Design League recently hooked up with Austin City Limits to create a poster for their Vampire Weekend taping. It’s an 18″ x 24″ screenprint, has an edition of 250 (much less online), and costs $30. Visit DeliciousDesignLeague.com.


Q:

SQL-How to split multiple items from 1 row to multiple rows

I'm working on creating a report that will tell me how many emails are assigned to a report subscription , for example a report called "Monthly Customer Activity - Default Setup" can have 2 email subscriptions and each subscription can have different email addresses , lets say 5 emails each subscription , at the end my report will display on one column 10 times the report Name and on the other column the 10 email addresses.
I'm working using the code below and is working most of the time but on some  rows is not doing the email split . I'm using SQL Management Studio 2016 and I'm connecting to my SSRS 2016 Database
Use [ReportServer]
IF OBJECT_ID('tempdb..#Temp') ISNOT NULL
drop table #Temp

select
c.Name,
-- 'two' as OtherID,
Convert(XML,[ExtensionSettings]).value('(//ParameterValue/Value[../Name="TO"])[1]','nvarchar(max)') as email
INTO #Temp
FROM
 dbo.[Catalog] c
INNER JOIN dbo.[Subscriptions] S ON c.ItemID = S.Report_OID
INNER JOIN dbo.ReportSchedule R ON S.SubscriptionID = R.SubscriptionID
INNER JOIN msdb.dbo.sysjobs J ON Convert(nvarchar(128),R.ScheduleID) = J.name
INNER JOIN msdb.dbo.sysjobschedules JS ON J.job_id = JS.job_id

;
WITH tmp(/*SomeID, OtherID,*/ Name, email,string) AS
(
    SELECT
        Name,
        --OtherID,
        LEFT(email, CHARINDEX(';', email + ';') - 1),
        STUFF(email, 1, CHARINDEX(';', email + ';'), '')
    FROM  #Temp

    UNION all

    SELECT
        Name,
        --OtherID,


    LEFT(email, CHARINDEX(';', email + ';') - 1),
        STUFF(email, 1, CHARINDEX(';', email + ';'), '')
    FROM tmp
    WHERE
        email != email
      and email is not NULL
)

select * from #Temp
order by email

The desired results will be:
https://imggmi.com/full/2019/1/17/a528cf472a96f5d2eff2759413b79814-full.png.html
Results I'm getting:
https://imggmi.com/full/2019/1/17/4ae835b3a877ad015af10372cf7f82e9-full.png.html
As you can see on the picture , is doing the split email on some rows but some others are still showing the emails together
Below is a sample test:
IF OBJECT_ID('tempdb..#Temp') IS NOT NULL drop table #Temp
CREATE TABLE #Temp
(Name varchar (50) , LastStatus varchar (max) , IBOAccount varchar (10) , Email varchar (max))
GO
INSERT INTO #Temp
Select 'Report A','Email sent to Email1@email.com','47213','Email1@email.com' UNION ALL
Select 'Report A','Email sent---- Minecraft Crash Report ----

// Would you like a cupcake?

Time: 9/16/14 10:51 PM

Description: Ticking screen

java.lang.IllegalStateException: Server tried to update attributes of a non-living entity (actually: EntityPrimalOrb['Orb of Primal Energy'/290714, l='MpServer', x=-274.50, y=71.00, z=-1126.50])

at net.minecraft.client.multiplayer.NetClientHandler.func_110773_a(NetClientHandler.java:1829)

at net.minecraft.network.packet.Packet44UpdateAttributes.func_73279_a(SourceFile:68)

at net.minecraft.network.TcpConnection.func_74428_b(TcpConnection.java:462)

at net.minecraft.client.multiplayer.NetClientHandler.func_72551_d(NetClientHandler.java:281)

at net.minecraft.client.multiplayer.GuiConnecting.func_73876_c(GuiConnecting.java:51)

at net.minecraft.client.Minecraft.func_71407_l(Minecraft.java:1580)

at net.minecraft.client.Minecraft.func_71411_J(Minecraft.java:910)

at net.minecraft.client.Minecraft.func_99999_d(Minecraft.java:838)

at net.minecraft.client.main.Main.main(SourceFile:101)

at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method)

at sun.reflect.NativeMethodAccessorImpl.invoke(Unknown Source)

at sun.reflect.DelegatingMethodAccessorImpl.invoke(Unknown Source)

at java.lang.reflect.Method.invoke(Unknown Source)

at net.minecraft.launchwrapper.Launch.launch(Launch.java:131)

at net.minecraft.launchwrapper.Launch.main(Launch.java:27)

A detailed walkthrough of the error, its code path and all known details is as follows:

---------------------------------------------------------------------------------------

-- Head --

Stacktrace:

at net.minecraft.client.multiplayer.NetClientHandler.func_110773_a(NetClientHandler.java:1829)

at net.minecraft.network.packet.Packet44UpdateAttributes.func_73279_a(SourceFile:68)

at net.minecraft.network.TcpConnection.func_74428_b(TcpConnection.java:462)

at net.minecraft.client.multiplayer.NetClientHandler.func_72551_d(NetClientHandler.java:281)

at net.minecraft.client.multiplayer.GuiConnecting.func_73876_c(GuiConnecting.java:51)

-- Affected screen --

Details:

Screen name: ~~ERROR~~ NullPointerException: null

-- Affected level --

Details:

Level name: MpServer

All players: 1 total; [EntityClientPlayerMP['Kuges'/290712, l='MpServer', x=-226.70, y=37.62, z=-1147.76]]

Chunk stats: MultiplayerChunkCache: 0

Level seed: 0

Level generator: ID 00 - default, ver 1. Features enabled: false

Level generator options:

Level spawn location: World: (8,64,-2237), Chunk: (at 8,4,3 in 0,-140; contains blocks 0,0,-2240 to 15,255,-2225), Region: (0,-5; contains


Q:

is there an Accurev CLI command for deep overlapped files specifically?

Im looking for a command that gives me all the deep overlapped files in a stream.
I've tried
accurev stat -B *.cs

Doesn't really work.

A:

Have you tried
accurev stat -B -o *.cs

A:

You should pass in the stream name where you want to search for deep overlaps as well as the -o switch for overlaps.
Example:  accurev stat -s stream_name_to_search_in -o -B

of FANCI and FANCD2 upon suppression of SF3B1 by RNAi. SF3B1 depletion also led to the decrease of FANCA (Fig. S1 C) and Chk1 levels (see [Fig. 8 B](#fig8){ref-type="fig"}). In contrast, other replication and repair proteins such as Nbs1, TopBP1, and PCNA were not significantly affected (Fig. S1 C). Treatment with the proteasome inhibitor MG132 did not rescue FANCI, FANCD2, and FANCA protein levels (Fig. S1 C). We did not detect FANCI and FANCD2 mRNAs in SF3B1-depleted cells (Fig. S1 D), indicating that the reduced protein level was the consequence of a defect in *FANCI* and *FANCD2* pre-mRNA processing. Consistent with this, expression of an intronless FANCI cDNA restored FANCI protein levels in SF3B1-depleted cells (Fig. S1 E). Collectively, these data indicate that SF3B1 is critical for expression


or not with CSK buffer with or without RNase A, or after 1-h preincubation with 10 µM triptolide. (Right) Mean intensity of signals from three independent experiments (minimum of 200 cells each); error bars indicate SD. DNA (blue) dyed with DAPI. (B) SC35 (red) IF and PLA signals (green) in U2OS cells. DNA (blue) dyed with Hoechst. Discontinuous circles indicate telophases (Tel). (C) IB showing total levels of SF3B1 and SC35 proteins in siRNA-transfected U2OS cells. (D) Representative images of the three arbitrarily established categories indicative of the different intensities of SC35 levels in the initial three stages of mitosis. These categories were used to assign cells quantified in E. (E) SC35 speckles IF in siRNA transfected U2OS cells. Visual quantification from three independent experiments. For the finaland Fig. S2 B). Hence, FANCD2 and FANCI exhibit distinct modes of association with SF3B1. The data so far indicate that FANCI associates with SF3B1 in chromatin and nucleoplasm during interphase and in the cytoplasm during mitosis, whereas the association between FANCD2 and SF3B1 is restricted to chromatin, most likely occurring during cotranscriptional splicing.

FANCI and FANCD2 regulate the mobility of prototypical SFs {#s05}
----------------------------------------------------------

The majority of transcription is suppressed during mitosis as a result of posttranslational modifications and chromosome hypercondensation ([@bib25]). To test whether FANCD2 and FANCI chromatin functions contribute to the timely eviction of SFs, we transfected U2OS cells with FANCI and FANCD2 siRNAs and visualized SC35 nuclear speckles in mitotic cells. Suppression of FANCI or FANCD2 did not perturb the level of SF3B1 and SC35 proteins ([Fig.


the postcatalytic spliceosome dismantler DHX15 (Fig. S3 B). DHX15, however, is also present in early spliceosomes ([@bib2]). Thus, we studied the interaction of FANCI and FANCD2 with three other spliceosome components: SPF45, whose distribution in spliceosomal complexes mirrors exactly that of DHX15 at very early stages ([@bib2]); TFIP11, exclusively present during disassembly of postcatalytic spliceosomes ([@bib57]; [@bib61]; [@bib65]); and PRP8, a key and central splicing component ([@bib2]).

We detected DHX15 and the early spliceosomal protein SPF45 in Flag-FANCI IPs, whereas the mid- and late-acting factors PRP8 and TFIP11 were not enriched in Flag-FANCI pull-downs ([Fig. 5 A](#fig5){ref-type="fig"}, compare lanes 3 and 4). PLA yielded concordant results ([Fig. 5 B](#fig5){ref-type="fig"}). In contrast, FANCD2 coimmunoprecipitated with DHX15 and the late acting factor TFIP11, but not with the early and mid-acting factorscontrol in Fig. S3 A). The overall analysis of speckles marked by SC35 or SF3B1 staining indicates that FANCI, but not FANCD2, promotes the mobilization of SFs from nuclear speckles in response to HU. This is consistent with the observation that FANCI and SF3B1 yield strong PLA signals in the nucleoplasm, whereas proximity between FANCD2 and SF3B1 is restricted to chromatin ([Fig. 2 A](#fig2){ref-type="fig"}). Upon inhibition of transcription, SFs accumulate in nuclear speckles, which consequently become larger and more rounded ([@bib39]). Consistent with proximity between FANCD2, FANCI, and SF3B1 depending on transcription activity or its product RNA ([Fig. 2 A](#fig2){ref-type="fig"}), the depletion of neither FANCI nor FANCD2 altered the rounding and size increase of speckles induced by the transcription inhibitor triptolide ([Fig. 7 C](#fig7){ref-type="fig"}; treatment control in [Fig.


reagent included in the kit) for 20 min at RT. Incubation with the mix of secondary antibodies was performed in a humid chamber for 1 h at 37°C. Slides were subsequently washed twice for 5 min with buffer A (150 mM NaCl, 10 mM Tris-Base, and 0.5% Tween 20, pH 7.4) before incubation with 20 µl ligation mix for 30 min at 37.0°C. Next, the slides were washed twice for 2 min with buffer A before incubation with 20 µl amplification mix for 100 min at 37°C in the humid chamber. The slides were washed twice for 10 min in buffer B (200 mM NaCl and 400 mM Tris-Base, pH 7.5) and then briefly in water before embedding the slides with DAPI DNA staining and PLA signal preservingwith 50 mM NaOH and 10 mM NaCl for 20 min, and then with 10 mM Tris-HCl, pH 7.5, for 20 min. The gels were equilibrated in 20× SSC (3 M NaCl and 0.3 M Na~3~Citrate.2H~2~O, pH 7) for at least 30 min. Transfer to a Hybond XL membrane (RPN303S; GE Healthcare) was achieved by capillarity. Nucleic acids were fixed to the membrane by UV-irradiating each side with 70,000 µJ/cm^2^. For hybridization, membranes were equilibrated for at least 1 h at 65°C in 0.5 M NaPO~4~, pH 7, and 7% SDS. The probe to detect linear GAPDH was designed to target exon 5. It was PCR-amplified from retrotranscribed RNA using the following primers: forward, 5′-ATTTGGTCGTATTGGGCG-3′, and reverse, 5′-AGTTGAGGTCAATGAAGGG-3′. The probe was labeled by random priming, purified by means


of MicroSpin G-50 columns (27-5330-01; GE Healthcare), heat-denatured, and added to the same solution. Incubations were performed overnight in a rotating oven at 65°C. The next morning, we performed three washes with 0.1× SSPE, 5 mM EDTA, pH 8, and 0.5% SDS at 65°C for at least 1 h. Radioactive signals were captured using a Fujifilm imaging plate and visualized using a Typhoon TRIO+ (Amersham).

Retrotranscription {#s33}
------------------

Retrotranscription reactions (20 µl) were performed using random primers on total RNA (500 ng) extracted using the GenElute Mammalian Total RNA Miniprep kit (RTN70; Sigma-Aldrich) or on immunoprecipitated RNA (1--10 µl of final product). In both cases, we used the SuperScript III reverse transcription kit from Invitrogen and followed the manufacturer's indications. Alternative splicing events were scored using the following primers: FAM62B (forward,into three samples, and volume was taken up to 1 mL with dilution buffer (0.01% SDS, 1.1% Triton X-100, 1.2 mM EDTA, 16.7 mM Tris, pH 8, 167 mM NaCl, and RNasin added for RNA-IPs) and incubated overnight with anti-SF3B1, anti-FANCD2, and anti-IgG antibodies on a rotating wheel at 4°C. Immunoprecipitations were performed during the course of a 2-h incubation at 4°C using 12 µl Protein A Magnetic Beads (10001D; Life Technologies) equilibrated in dilution buffer and saturated with 1 mg/ml BSA. Next, the supernatant was discarded, and the beads were washed for 10 min at 4°C with 700 µl RIPA buffer (150 mM NaCl, 50 mM Tris, pH 8, 0.1% SDS, 0.5% NaDoc, and 1% NP-40) supplemented with RNAsin, followed by high salt buffer (500 mM NaCl,


F. Rosselli, and Y-L. Lin for reagents; R.F. de Luco, M-H. Stern, R. Kiernan, A. Aguilera, C. Ribeyre, and J. Basbous for suggestions and critical reading; the Montpellier Rio Imaging facility; J. Cau and R. Forey for providing tools for IF quantification; and P. Pasero and B. Pardo for support.

Fondation ARC pour la Recherche sur le Cancer (CT060509), Institut National du Cancer (2015-1-PL BIO-03), and MSD Avenir (GnoStiC) supported this work. S. Ovejero is the recipient of a fellowship from Ligue Contre le Cancer.

The authors declare no competing financial interests.

Author contributions: M. Moriel-Carretero, S. Ovejero, and M. Gérus-Durand: conceptualization, data curation, investigation, methodology, and writing. D. Vryzas: investigation. A. Constantinou: conceptualization, funding acquisition, supervision, validation, writing, review, and editing.

Abbreviations used:CSKcytoskeletonCVcolumn volumeDDRDNA damage responseFAFanconi anemiaHUhydroxyureaIBimmunoblottingICLinterstrand cross-linkIFimmunofluorescenceIPimmune precipitatePIprotease inhibitorPLAproximity ligationPG biotransformation by SRB (bioremediation process), its commercial development has been substantially limited by certain major challenges. These include the efficient reduction or immobilization of co-contaminants (i.e., trace metals, radionuclides), the use of low-cost and abundant renewable water sources for sulfate reduction (e.g., wastewaters and/or seawater), and the improvement of sulfate reduction yields through the development of optimal control of mixed SRB cultures. Despite the abundance of SRB in marine ecosystems and their efficiencies in PG biotransformation, the biotechnological potential, and diversity of marine SRB, originating from PG-polluted coastal marine ecosystems, to carry out such reductive process have not yet been studied.

The aim of this study was to determine the potential of SRB populations originating from marine sediments, to biotransform PG. To this end, we first evaluated the


initial microbial community diversity of PG-polluted marine sediments, close to a coastal phosphate fertilizer plant, as well as that of the Tunisian PG, characterized by a low pH (\~3). Marine SRB were then enriched and cultivated from PG-polluted sediments, but not directly from PG (as inoculum). Finally, we analyzed the SRB diversity of marine sediments associated with changes in H~2~S production from PG or sodium sulfate as sulfate sources using various electron donors (e.g., acetate, formate, or lactate).

Materials and methods {#s2}
=====================

Sample collection and characteristics
-------------------------------------

PG and sediment samples were collected in March 2016 in the phosphate fertilizer complex of Sfax City, southern Tunisia. The Sfax plant (SIAPE factory) stockpiles \~30 million tons of PG 1 km inland from the coast. The PG stockpile covers more than 48 ha andoccur in the extreme conditions prevailing in PG. *Archaea* in PG accounted for 1.53% of the microbial community and were mainly affiliated with the *Euryarchaeota* phylum (Table [S6](#SM6){ref-type="supplementary-material"}). The most abundant archaeal OTUs from PG were distantly related to *Methanomassiliicoccus luminyensis*, reported as a hydrogenotrophic methanogen using methanol as terminal electron acceptor (Dridi et al., [@B15]). *M. luminyensis* were also detected in low-pH environments impacted by acid mine drainage (Mendez-Garcia et al., [@B37]).

Microbial H~2~S production using sodium sulfate or phosphogypsum as sulfate source
----------------------------------------------------------------------------------

During the 14-day experiment, H~2~S was produced by the MS microbial community using Na~2~SO~4~ or PG as sulfate sources (Figure [2](#F2){ref-type="fig"}), indicating that SRB originating from MS can efficiently use PG. As expected, low H~2~S production was obtained in MS controls without addition of electron donors.


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Agent Comments: Wood hill is the quintessential middleburg property in the heart of fox hunting country. Ideally located just 3 miles from the quaint village of middleburg. This 49 acre property has an elegant 1940's brick colonial home, stable, cottage, apartment,GO has already done several electrification studies, this is the first to look at the entire 509-kilometre rail network. It is also the first to separate out the costs of electrification from the incremental costs of improving and maintaining the GO system.

“The cost to electrify the whole system will be less than it has been conveyed previously because there tended to be a mixing of those numbers,” said Pitre, who wouldn’t provide any hint of the cost estimates before the study is released publicly, most likely in January.

The study looks at six electrification options and the environmental and ridership impact for each.

A 2001 study put the cost of electrifying the Lakeshore line between Hamilton and Oshawa at about $300 million. GO officials have estimated that electrifying the entire


network would cost between $1 billion and $2 billion.

It’s already clear that electrifying entire GO lines makes more sense than converting individual sections of track, said Pitre.

“The previous studies have shown (electrification) can work. The question is — how big are the benefits? There’ll be a debate about that,” said Pitre.

Electric trains emit fewer harmful pollutants than diesel engines and can speed trip times, potentially shaving up to 12 minutes off a Georgetown trip and seven minutes off a journey to Hamilton.

But electric trains would still be subject to constraints that limit GO service now, including competing freight traffic, speed restrictions and limited platforms at Union Station.

Meantime, Ontario NDP leader Andrea Horwath has called on the Liberal government to stop Metrolinx from buying American-made Japanese diesel trains.

Toronto’s Cleandevelopment and injury response. Cellular senescence also contributes to the declines in the regenerative capacity and function of tissues, inducing inflammation, and pathological remodeling in aged organs ([@B15]). The cardiomyocytes are terminus-differentiated cells. Cell cycle arrest is not the hallmark of cardiomyocyte senescence. As thus, the senescence of the cardiomyocytes is not easy to give a precise definition as the proliferative or stem cells. Actually, the senescence of cardiomyocytes is generally accomplished with various functional declines, including DNA damage response, ER stress, mitochondria dysfunction, contractile dysfunction, hypertrophic growth, and SASP. Senescent cardiomyocytes also express β-galactosidase ([@B16]), which is prominent in senescent cells ([@B15]). Here, we summarize these hallmarks in senescent cardiomyocytes ([Figure 1](#F1){ref-type="fig"}).

![Hallmarks of cardiomyocyte senescence. Senescence of cardiomyocytes exhibits the features of DNA damage and genomic instability,


with each other and lead to a dysfunctional heart in the pathological conditions. Reciprocally, signals released by non-myocytes cells also affect cardiomyocytes and contribute to cardiomyocyte senescence ([Figure 2](#F2){ref-type="fig"}).

![Communications between cardiomyocytes and non-myocytes within the microenvironment. Signals released by non-myocytes contribute to cardiomyocytes senescence. The dysfunctional endothelial cells (ECs) secrete pro-inflammatory factors (TGF-β, IL-6, and IL-33), ET-1, and Ang II to modulate cardiomyocyte senescence. Extracellular ventricles (EVs) or exosomes produced by ECs that consist of Mst1 and microRNAs also regulate the function of cardiomyocytes. Fibroblasts modulate cardiomyocyte senescence by paracrine signaling and remodeling of extracellular matrix (ECM). Fibroblasts express matrix metalloproteinases (MMPs), integrins, and fibronectin to interact with ECM, which are critical for paracrine signaling. Proinflammatory factors including IL-11, IL-33, and EVs composed of miR-21-3p, osteopontin, and EGFR(PGE2), parathyroid hormone-related peptide (PTHRP), nitric oxide (NO), endothelin 1 (ET-1), and neuregulin-1 (NRG1) to promote the proliferation and maturation of cardiomyocytes ([@B62]). In the condition of metabolic dysfunction and cardiac remodeling, endothelial cells secrete Ang II, NRG1, ET-1, apelin, and proinflammatory factors (e.g., TGFβ and IL6), and eicosanoids to modulate the senescence of cardiomyocytes ([@B62]). For instance, high expression and release of Ang II and ET-1 by endothelial cells can stimulate the mitochondrial dysfunction, ER stress, contractional dysfunction, and hypertrophic growth of cardiomyocytes *via* binding their receptors on cardiomyocytes. In the cardiovascular system, ET-1 is the most potent vasoconstrictor that has remarkably long-lasting actions. ET-1 is an important contributor to vasoconstriction, vascular and cardiac hypertrophy, as well as tissue inflammation, and subsequently participates in the development and


interactions that regulate cardiomyocyte senescence and pathological adaptations ([@B12], [@B85]). Fibronectin polymerization is necessary for collagen matrix deposition. Fibronectin is a central contributor to the increased abundance of cardiac myofibroblasts during cardiac injury and remodeling. Fibronectin polymerization inhibitor (pUR4) or genetic deletion of fibronectin in fibroblasts repressed the cardiomyocyte hypertrophy and heart failure ([@B86]). Cardiac calcification is common with age and injury, which leads to heart blocks. Cardiac fibroblasts adopt an osteoblast cell-like fate and regulate cardiomyocyte calcification directly ([@B87]).

Metabolic modulators regulate the function of fibroblasts in cardiac aging and remodeling. For instance, adiponectin activates the APPL1-AMPK signaling and induces cell migration, MMP activation, and collagen remodeling in cardiac fibroblasts ([@B88]). AMPK activation increases the content of fibroblasts in the infarcted area ([@B89]). FOXO3A mediates Peroxiredoxin III expression,which plays a critical role in the resistance to oxidative stress in cardiac fibroblasts. In addition, FoxO1 contributes to TGFβ-dependent cardiac myofibroblast differentiation ([@B90]). As thus, metabolic regulations within cardiac fibroblasts may also participate fundamentally in regulating the dysfunction and senescence of cardiomyocytes.

Immune Cells Contribute to Cardiomyocyte Senescence
---------------------------------------------------

Immune cells play a crucial role in tissue homeostasis and pathogenesis. Immune cells in the cardiac tissues can regulate inflammatory response and modulate cardiomyocyte senescence directly. These immune cells include macrophages, T cells, and mast cells.

A large number of studies have identified the roles of macrophages in the physiological and pathological progress of cardiac tissues. Macrophages participate in cardiac development, regeneration, and pathological remodeling ([@B91], [@B92]). Cardiac macrophages promote the electrical conduction of cardiomyocytes through the distal atrioventricular node, where conducting


cells closely intersperse with elongated Connexin 43-positive macrophages. Macrophage-specific depletion of Connexin 43 induces cardiomyocyte senescence with dysfunctional electrical activity ([@B93]). Cardiac macrophages with NLRP3 inflammasome activation secreted IL-1β, which promotes cardiomyocyte senescence by causing prolongation of the action potential duration, inducing a decrease in potassium current and an increase in calcium sparks in cardiomyocytes of diabetic mice ([@B94]). A similar role of macrophages was also observed in atrial fibrillation ([@B95]). Iron regulator hepcidin impairs macrophage-dependent cardiac repair after injury. *Hepcidin* deficiency increased the content of chemokine (C-C motif) receptor 2 (CCR2)^+^ inflammatory macrophages. These macrophages fostered signal transducer and activator of transcription factor-3 (STAT3) phosphorylation to release IL-4 and IL-13, and to favor cardiomyocyte renewal ([@B96]). Macrophage-mediated inflammation also contributes to cardiomyocyte hypertrophy ([@B11]). Tissue-resistant macrophages alsocontribute to the recruitment and activation of other immune cells ([@B97]). Therefore, macrophages are critically involved in cardiomyocyte renewal and senescence. However, it remains to be documented which subtypes of macrophages contribute to self-renewal and which subtypes of macrophages contribute to cardiomyocyte senescence, and the specific paracrine factors are also needed to be investigated.

T cells play a central role in adaptive immunity, but their functional roles in cardiac pathophysiology are still poorly understood. δT lymphocytes predominantly produce IL-17A. During myocardial ischemia/reperfusion, IL-17A promotes cardiomyocyte apoptosis ([@B98]). T cell costimulation blockade inhibits cardiomyocyte apoptosis and blunts pressure overload-induced hypertrophic growth of cardiomyocytes and heart failure ([@B99]). Adoptively transferred CD4^+^CD25^+^ regulatory T (Treg) cells ameliorate Ang II-induced cardiac damage and fibrosis in the hypertensive heart ([@B100]). Recently, a study reported


rod assembly even at elevated temperatures. To obtain this force, the coil must have sufficient turns of wire in the coil capable of providing a large current. The turns should be in close contact to reduce losses. For application to a liquid metal cooled reactor the insulation must withstand temperatures at 600.degree. C. continuously, with possible temperature excursions as high as 750.degree. C. Additionally, the coil should be a strong monolithic structure capable of withstanding electrical and vibrational forces as well as radiation doses of at least 10.sup.7 rads.
2. Description of the Prior Art
Many different materials are commonly used to coat or cover electrical wire for the purpose of providing electrical insulation. Insulation presently known to be prior art is generally unsuited for use in an environment offor but every time the movie seems to have got its act together, it comes unstuck by some odd comedic choices.

Owen Wilson’s unscrupulous retirement home boss is a poorly defined character, for instance, whose wise-cracking at the expense of patients (including pot-shots at cancer) and general mis-treatment of them flies pretty close the mark. While his second-hand man, played by Eddie Griffin, is similarly woeful and merely highlights what a one-dimensional performer Griffin has become of late.

Attempts to balance the hick comedy of My Name Is Earl with the surrealism of regular Wilson cohort Wes Anderson also feels awkward and not in its best interests.

But for anyone who sticks with it, there are laughs to be had from Wilson and Ferrell’s rivalry, as well as some poignant interaction


Andrea Rasmussen

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I am looking for amy type of job. I have expierence in most things. I have worked in a CBRF (Assisted living) building and I have certificates for that also. I have Housekeeping experience and I have young children so I could work with kids also. I was also a detailer.the separate definition.

Colleges and universities

In the House’s version of tax reform, private colleges with endowments valued at $250,000 per full-time student would be subject to a 1.4 percent excise tax. The Senate version places the same tax only on larger endowments, those valued at $500,000 per full-time student.

Both the House and the Senate bills scrap a rule that allows taxpayers to write off 80 percent of the amount paid on tickets to college sporting events, which is currently allowed as a charitable deduction.

Two other elements of tax reform do not apply specifically to colleges and universities, but have the potential to have a large impact on them.

Both the House and Senate tax bills nearly double the standard deduction for taxpayers, which could prevent many taxpayers from itemizing. Charitable


Q:

TYPO3 v8.5 with Fluidtemplate and fluid-styled-content - Partials not working

I use TYPO3 v8.5 with fluid-templating (with layout, partials and template) and fluid-styled-content.
However, as soon as I create a fsc-element e.g. text&media on my site, the partial files are ignored and only the section from the template file is output in the FE.
If I set the content-element to "hide", then the correct site-structure with partials were output in the FE.
My root-template is:
page = PAGE
page.includeCSS {
  main = fileadmin/Demo/Resources/Public/Css/main.css
}
page.10 = FLUIDTEMPLATE
page.10 {
    layoutRootPaths {
        10 = fileadmin/Demo/Resources/Private/Layouts
        20 = fileadmin/Demo/Individual/Private/Layouts
    }
    partialRootPaths {
        10 = fileadmin/Demo/Resources/Private/Partials
   might lose him forever... or at least just have to restart from the last safe room.

7. Determination

It turns out you need to take Chompski with you all the way to the end of the campaign, not just the end of the one level. So I put a bit more on my plate than I was expecting when I first decided to get this achievement. Luckily, if you die in a later level, then you can simply start over from the begging in the safe room with Chompski again. Needless to say, I ended up dying on purpose a couple of times just to get Chompski back.

Fun Fact!: Gnome Chompski is named after Noam Chomsky.

You probably already knew this, but just in case you didn't ...

Noam Chomsky was a


g: 2, e: 1, y: 2}. Give prob of picking 1 e, 1 v, and 1 y.
1/14
Calculate prob of picking 4 f when four letters picked without replacement from {t: 1, s: 2, f: 4, v: 1}.
1/70
Four letters picked without replacement from ssnpaanasnnnnnssnps. What is prob of picking 1 p, 2 a, and 1 s?
3/323
What is prob of picking 1 g and 2 h when three letters picked without replacement from {h: 5, g: 9}?
45/182
Two letters picked without replacement from nuqarqrarr. What is prob of picking 1 q and 1 n?
2/45
Calculate prob of picking 2 d when two letters picked without replacement from {d: 11, a: 9}.
11/38
Calculate prob of picking 4 g when four letters picked without replacement from {g: 9, w: 10}.
21/646
What is prob of picking 2of picking 1 z and 1 k when two letters picked without replacement from {e: 2, k: 2, j: 1, f: 4, z: 1}?
2/45
Two letters picked without replacement from {i: 2, f: 2, z: 5, g: 4}. Give prob of picking 2 z.
5/39
Calculate prob of picking 1 w, 1 a, and 2 e when four letters picked without replacement from {a: 1, w: 3, x: 4, s: 2, b: 3, e: 2}.
1/455
Three letters picked without replacement from vvmvaavmv. Give prob of picking 3 v.
5/42
Two letters picked without replacement from hhyhyhhyhhyyhyyhyyy. What is prob of picking 2 y?
5/19
Two letters picked without replacement from {e: 1, u: 1, b: 4, i: 1, n: 1}. What is prob of picking 1 i and 1 n?
1/28
Three letters picked without replacement from ssssssesesse.


data collection. Likert scale questions are limited from the perspective of being unidimensional. In other words, they only gave the respondents certain amount of choices, implying that the space between each possibility is equidistant, which is not always the case in real life. However, the benefits of Likert scale questions outweigh this main limitation. For example, Likert scale is a universal method of collecting data, mainly because it is easy to use by researchers and also easy to understand by respondents. The responses to the questions are easily quantifiable, and since it does not require the respondents to provide a simple and concrete yes or no answer, it does not force them to take a stand on a particular topic but allows them to respond in a degreea quantifiable way without allowing for manipulation of respondents' outcomes.\[[@ref9]\]

Funding {#sec2-6}
-------

There was no funding sought for this piece of research, as monetary value can create bias. To further minimize bias, maximum effort was deployed at the sample selection stage to achieve more interest than may be needed as large-scale dropout from the study could have had adverse effect on the study outcome.

Ethical considerations {#sec2-7}
----------------------

Ethics is the aspect of moral philosophy, which helps professionals to consider what will be right (ethical) or what will be wrong (unethical) in their action and thus guide their decisions.\[[@ref34]\] Some scholars in reference to research ethics stated that ethics is concerned with the degree to which research procedures adhere to professional, legal and social obligations to the study participants.\[[@ref9]\] The responsibilities of academic


former boss Sam Presti and blow up the Magic roster in favor of building through the draft. Vecsey believes that Howard still desperately wants to go to the Brooklyn Nets to pair up with point guard Deron Williams to compete for a NBA title.

It is certainly possible that Hennigan will look to move Howard soon, but his first move will be to meet with the talented big man.

Personally I look forward to sitting down with Dwight and sharing with him the vision and the direction we're going to go, Hennigan told the Associated Press, and I look forward to listening to what his thought process is and where his head's at. At the end of the day, we'll take it from there.

The Nets have been desperate to acquire" ";
    String initNumber = " ";
    float number = 0;
    int decimals = 0;

    System.out.println("Welcome to the square root program.");
    do{
        addLine();
        System.out.print("Please enter the number of which you want to find the square root:  ");
        initNumber = reader.readLine();
        number = Float.valueOf(initNumber);
        System.out.println(number);
        System.out.print("Please enter the number of decimal places in which you want your answer:  ");
        decimals = reader.readInt();


    //We have found the first digit that is less than the remainder!
               remainder -= trial;
               ans += j;
               break;
          }
     }
}

Now all that remains is to send back the answer:
return Double.parseDouble(ans.substring(0, decimalIx) + "." + ans.substring(decimalIx));

Here is the full code.
public static double squareRootDiv(String number, int decimals){
        if (decimals <= 0) return -1;

        String ans =generation:

* You think you are being terrorized by other people, but in truth you are being terrorized by your beliefs. Your experience of yourself and your world will shift dramatically if you adopt the Five Steps to Peace:

(1) Permit yourself to acknowledge that some of your old beliefs about God and about Life are no longer working.

(2) Explore the possibility that there is something you do not fully understand about God and about Life, the understanding of which would change everything.

(3) Announce that you are willing for new understandings of God and Life to now be brought forth,understandings that could produce a new way of life on this planet. read more…

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Is there no solution to the North Korean-U.S. standoff? Yes, there is. But the


Restore - Gut Health Mineral Supplement

Restore's proprietary lignite extract was developed to support the integrity of your intestinal walls. This creates a stable environment where good gut bacteria can grown and thrive, helping you to promote immune system function, and supporting optimal well-being.defmodule MiphaWeb.Endpoint do
  use Phoenix.Endpoint, otp_app: :mipha

  socket "/socket", MiphaWeb.UserSocket,
    # or list of options
    websocket: true,
    longpoll: false

  # Serve at "/" the static files from "priv/static" directory.
  #
  # You should set gzip to true if you are running phoenix.digest
  # when deploying your static files in production.
  plug Plug.Static,
    at: "/",
    from: :mipha,
    gzip: false,
    only: ~w(css fonts images js favicon.ico robots.txt)

  # Code reloading can be explicitly enabled under the
  # :code_reloader configuration of your endpoint.
  if code_reloading? do
    socket("/phoenix/live_reload/socket", Phoenix.LiveReloader.Socket)
    plug Phoenix.LiveReloader
    plug


solution to a rough differential equation of the form $$\begin{aligned}
  {\,\mathrm{d}v(t)} = b(v(t)) {\,\mathrm{d}t} + {\,\mathrm{d}r(t)}, \quad v(0) = v_0,\end{aligned}$$ where $r \colon [0,T] \to {{\mathbb{R}}}^d$ is a non-smooth but integrable perturbation. Then, the mapping $u \colon [0,T] \to {{\mathbb{R}}}^d$ given by $u(t) := v(t) - r(t)$, $t
\in [0,T]$, solves an ODE of the form with $f(t,x) := b(r(t) +
x)$ for each $(t,x) \in [0,T] \times {{\mathbb{R}}}^d$. Depending on the smoothness of the perturbation $r$, the resulting mapping $f$ is then often only integrable or Hölder continuous with exponent $\gamma \in (0,1]$ with respect to the temporal variable $t$.

Due to the low regularity of the coefficient function $f$ the numerical approximation of the solution $u$ to Carathéodory type differential equations is a challenging task. Indeed, it cannumerical approximation. For a proof and more general variants see for instance Proposition 4.1 in [@emmrich1999].

\[lem:Gronwall\] Consider two nonnegative sequences $(u_n)_{n\in {{\mathbb{N}}}}$ and $(w_n)_{n\in {{\mathbb{N}}}}$ which for some given $a \in [0,\infty)$ satisfy $$u_n \leq a + \sum_{j=1}^{n-1} w_j u_j,\quad \text{ for all }  n \in {{\mathbb{N}}},$$ then for all $n \in {{\mathbb{N}}}$ it also holds true that $$u_n\leq a \exp\Big(\sum_{j=1}^{n-1} w_j \Big).$$

For the introduction and the error analysis of Monte Carlo methods, we also require some fundamental concepts from probability and stochastic analysis. For a general introduction readers are referred to standard monographs on this topic, for instance [@kallenberg2002; @klenke2014; @oksendal2003]. For the measure theoretical background see [@bauer2001; @cohn2013].

First let us recall that a *probability space* $(\Omega,\mathcal{F},{{\mathbb{P}}})$ consists of a measurable space $(\Omega,\mathcal{F})$ endowed with a


the independence of $(\tau_j)_{j \in {{\mathbb{N}}}}$. In addition, we also obtain from that $$\begin{aligned}
    &\Big\| \int_{t_{j-1}}^{t_j} \big( g(s) - g(t_{j-1} + h
    \tau_j) \big) {\,\mathrm{d}s} \Big\|_{L^p(\Omega;{{\mathbb{R}}}^d)}\\
    &\quad \le \int_{t_{j-1}}^{t_j} | g(s) | {\,\mathrm{d}s}
    + h \big\| g(t_{j-1} + h \tau_j) \big\|_{L^p(\Omega;{{\mathbb{R}}}^d)} < \infty.
  \end{aligned}$$ Therefore, $(E^n)_{n \in \{1,\ldots,N_h\}}$ is a discrete time $L^p$-martingale. Thus, we can apply the Burkholder-Davis-Gundy inequality from Theorem \[th:BDG\] and obtain $$\begin{aligned}
    \big\| \max_{n \in \{1,\ldots,N_h\}} | E^n| \big\|_{L^p(\Omega;{{\mathbb{R}}})}
    \le C_p \big\| [E]^{\frac{1}{2}}_{N_h} \big\|_{L^p(\Omega;{{\mathbb{R}}})}.
  \end{aligned}$$ After inserting the quadratic variation $[E]_{N_h}$ we arrive at $$\begin{aligned}
    \label{eq4:est_E}
    \begin{split}
      &\big\| \max_{n \in \{1,\ldots,N_h\}}{{\mathbb{R}}}^d$ be a given measurable mapping with $\| g
  \|_{L^p([0,T];{{\mathbb{R}}}^d)} < \infty$ for some $p \in (2, \infty)$. Let $(h_m)_{m \in {{\mathbb{N}}}} \subset (0,1)$ be an arbitrary sequence of step sizes with $\sum_{m = 1}^\infty h_m < \infty$. Then, there exist a nonnegative random variable $m_0 \colon \Omega \to {{\mathbb{N}}}_0$ and a measurable set $A \in {{\mathcal{F}}}$ with ${{\mathbb{P}}}(A) = 1$ such that for all $\omega \in A$ and $m \ge
  m_0(\omega)$ we have $$\begin{aligned}
    \label{eq4:errRie3}
    \max_{n \in \{0,1,\ldots,N_{h_m}\}} \Big| \int_0^{t_n} g(s) {\,\mathrm{d}s} -
     Q_{\tau,h_m}^n[g](\omega) \Big| \le h_m^{\frac{1}{2} - \frac{1}{p}}.
  \end{aligned}$$ Moreover, if in addition $g \in \mathcal{C}^\gamma([0,T])$ for some $\gamma \in (0,1]$, then for every $\epsilon \in (0,\frac{1}{2})$ there exist a nonnegative random variable


1 + |u(s)| ) {\,\mathrm{d}s}\\
    &\le |u_0| + \int_0^T \overline{K}(s) {\,\mathrm{d}s} +
    \int_0^t \overline{K}(s) |u(s)| {\,\mathrm{d}s}.
  \end{aligned}$$ Then, an application of Gronwall’s inequality (see e.g. [@hale1980 Chap. I, Cor. 6.6]) yields the assertion .

Next, assume that $p \in (1,\infty)$ and let $0 \le s \le t \le T$ be arbitrary. Then, from and we further deduce that $$\begin{aligned}
    | u(t) - u(s) | &\le \int_s^t |f(z, u(z)) | {\,\mathrm{d}z} \le \int_s^t
    \overline{K}(z) ( 1 + |u(z)| ) {\,\mathrm{d}z}.
  \end{aligned}$$ Since $u$ is bounded and since the mapping $\overline{K}$ is $p$-fold integrable we obtain from the Hölder inequality with exponents $p$ and $p'
  = \frac{p}{p-1} \in (1,\infty)$ that $$\begin{aligned}
    | u(t)$\theta_j \sim \mathcal{U}([t_{j-1},t_j])$. Moreover, we observe that $\theta_j$, and therefore also $L(\theta_j)$, is independent of the errors at earlier time levels. Thus, from we obtain $$\begin{aligned}
    &\big\| L(\theta_j) \max_{i \in \{0,1,\ldots,j-1\}}
    \big| u( t_{i} ) - U^{i} \big|  \big\|_{L^p(\Omega;{{\mathbb{R}}})}^p\\
    &\quad = {{\mathbb{E}}}\big[ L(\theta_j)^p \big]
    {{\mathbb{E}}}\big[ \max_{i \in \{0,1,\ldots,j-1\}}
    \big| u( t_{i} ) - U^{i} \big|^p \big]\\
    &\quad = \frac{1}{h} \int_{t_{j-1}}^{t_j} L(s)^p {\,\mathrm{d}s}
    \big\|  \max_{i \in \{0,1,\ldots,j-1\}} \big| u( t_{i} ) - U^{i} \big|
    \big\|_{L^p(\Omega;{{\mathbb{R}}})}^p.
  \end{aligned}$$ Inserting this into yields $$\begin{aligned}
     &\big\| \max_{i \in \{0,1,\ldots,n\}} \big| u(t_i) - U^i \big|
     \big\|_{L^p(\Omega;{{\mathbb{R}}})}^p \\


estimated by .

Again, we take note of the fact that the error constants $C_U$ and $C_V$ both grow at least exponentially with the final time $T$ and the Lipschitz constant $L$. Both methods are therefore not necessarily well-suited for long-time simulations or if the ODE is stiff.

We close this section with the following result on the almost sure convergence of the randomized Euler method and the randomized Runge-Kutta method .

\[th5:error2\] Let Assumption \[as5:f\] be fulfilled for some $\gamma \in (0,1]$ and let $u$ denote the exact solution to . For a given sequence $(h_m)_{m \in {{\mathbb{N}}}} \subset (0,1)$ of step sizes with $\sum_{m = 1}^\infty h_m
  < \infty$ let $(U^j_{m})_{j \in \{0,1,\ldots,N_{h_m}\}}$ and $(V^j_{m})_{j \in \{0,1,\ldots,N_{h_m}\}}$ denote the numerical approximations determined by and with initial condition $u_0$ andand T. Swartz. . Oxford Statistical Science Series. Oxford University Press, Oxford, 2000.

P. K. Friz and M. Hairer. . Universitext. Springer, Cham, 2014. With an introduction to regularity structures.

R. D. Grigorieff. . B. G. Teubner, Stuttgart, 1972.

I. Gy[ö]{}ngy. A note on [E]{}uler’s approximations. , 8(3):205–216, 1998.

S. Haber. A modified [M]{}onte-[C]{}arlo quadrature. , 20:361–368, 1966.

S. Haber. A modified [M]{}onte-[C]{}arlo quadrature. [II]{}. , 21:388–397, 1967.

E. Hairer, S. P. N[ø]{}rsett, and G. Wanner. , volume 8 of [ *Springer Series in Computational Mathematics*]{}. Springer-Verlag, Berlin, second edition, 1993. Nonstiff problems.

J. K. Hale. . Robert E. Krieger Publishing Co., Inc., Huntington, N.Y., second edition, 1980.

S. Heinrich. Complexity of initial value problems in [B]{}anach spaces. , 9(1):73–101, 116, 2013.

S. Heinrich and B. Milla. The randomized complexity of initial value problems. , 24(2):77–88, 2008.

A. Jentzen and A. Neuenkirch. A random [E]{}uler scheme for [C]{}arathéodory differential equations. , 224(1):346–359, 2009.

B. Kacewicz. Optimal solution


Shedding of Toxoplasma gondii oocysts by Felidae in zoos in the Czech Republic.
In 1995 and 1996, the shedding of Toxoplasma gondii oocysts was monitored in the faeces of Felidae in six zoos in the Czech Republic. In all, 2287 samples of faeces from 19 species of Felidae were examined. In Ostrava Zoo, four episodes of shedding of Toxoplasma-like oocysts were identified, using a flotation examination, in a pair of wild cats (Felis silvestris), six episodes in a wild cat held separately, and three episodes in a pair of Amur leopard cats (F. euptilurus). After the passage of sporulated oocysts through laboratory mice, T. gondii was confirmed in the pair of wild cats (three episodes), in the wild cat held separately (three episodes) and in the pair of Amura GOP governor he has to engage a legislature controlled by Democrats to get things done.

"If I wait for them to lead on an issue, I'll be waiting my entire career," he said.

"The problem is that in the end the federal guys are not required to produce any results," Christie said. "People would never put up with this from a governor."

With the Super Bowl to be held at New Jersey's Meadowlands sports complex in February, McCain and Christie discussed efforts to cut down on the practice of prostituting minors, often from abroad and subjected to threats and other forms of coercion. He said the event is an opportune time to focus on the problem because so many law enforcement agencies are working together.

"This is something that has been


be making money – you can imagine not having that grizzly commute and just working from home someday, kicked back with your feet up, enjoying the journey as much as the thought of the eventual destination. The point is this could be critical someday, so find a reason to love it and pour whatever is left of yourself by the time you get home into it.

We can never know what is around the corner. Whether it is going to be something that helps or something that hinders. Therefore we need to be prepared, and making the most money now, while you still can, is a wise decision. There is a whole laundry list of possible disasters of varying magnitudes that could befall you and your family. If youwould be unfortunate, but will not ruin you. On the other hand gambling with the month’s rent money would be disastrous if you lost it, so don’t even go there. Don’t speculate, imagining you will get your money back in time to pay the rent. What if you don’t? Then what?

So starting with yourself you need to determine what you want to do, and you need a few more details than ‘to make money from home’. Do you like marketing and/or sales? If you have never done it before would you like to learn how and accept that there will be work involved and it may be unpaid work until you get going?

If you are not crazy about marketing and sales that is understandable and so you should


and were influential both in England and on the Continent. In his monasteries, learning reached a high standard, producing competent prose and poetry in the elaborate hermeneutic style of Latin favoured in tenth-century England. His Winchester school played an important role in creating the standard vernacular West Saxon literary language, and his pupil Ælfric was its most eminent writer.

All surviving medieval accounts of the movement are by supporters of reform, who strongly condemned what they saw as the corruption and religious inadequacy of the secular clergy, but historians in the late twentieth and early twenty-first centuries have increasingly seen these accounts as unfairly biased against the secular clergy.

Background
The author of the Rule of Saint Benedict, which was the principal monastic code in Western Europe in the early MiddleEnglish Benedictine reform were Dunstan, Archbishop of Canterbury (959–988), Æthelwold, Bishop of Winchester (963–984), and Oswald, Archbishop of York (971–992). Dunstan and Æthelwold reached maturity in Æthelstan's cosmopolitan, intellectual court in the 930s, where they met monks from the European reformed houses which provided the inspiration for the English movement. In the early 940s Dunstan was appointed Abbot of Glastonbury, where he was joined by Æthelwold, and they spent much of the next decade studying Benedictine texts at Glastonbury, which became the first centre for disseminating monastic reform. The Rule of Saint Benedict was translated into Old English at this time, probably by Æthelwold, and it is the only surviving prose translation of the Rule into a European vernacular in the early Middle Ages. In about 954 Æthelwold


shaving the pubic hair.” (Sahih Bukhari & Sahih Muslim)

With regards to your question, the obligation of the salah will be done but you will not receive the full reward of the salah.

Only Allah Knows Best

Mohammed Tosir Miah

Darul Ifta Birminghamher up to 50 years imprisonment.

The victim’s 37-year-old brother, Andrew, exclaimed that their family’s grief is further worsened by the fact that they’re unable to see his brother’s two children. “We’ve not seen or heard from the children since Michael’s passing because they were taken away by his ex-wife’s brother the next morning, when we are trying to grieve there is a fight to try and see the children. We just want to get them home.”

“Michael loved working but his overwhelming passion was his two children, Jack and Alice. When he was not working he would spend every minute of the day with them,” Andrew explained. Simpson’s two children are still in the care of Fu’s brother.

However, Simpson’s parents are now entangled in a legal battle in China,


don’t think Belmont gets past Georgetown. The Bruins shoot a ton of threes and shoot them well, but Georgetown has terrific perimeter length and is one of the best in the country at defending the three. I also think SDSU gets by NC State. I know the pro-Wolfpack arguments, but what have they done this season to make me believe they can win a tournament game against the co-champ of the fourth-best conference in the country?     "Governance: Requires _votingQuorumPercent between 1 & 100"
    );

    /**
     * @notice Address and contract instance of Audius Registry. Used to ensure this contract
     *      can only govern contracts that are registered in the Audius Registry.
     */
    Registry private registry;

    /// @notice Address of Audius staking contract, used to permission Governance method calls
    address private stakingAddress;

    /// @notice Period in blocks for which a governance proposal is open for voting
    uint256 private votingPeriod;

    /// @notice Number of blocks that must pass after votingPeriod


  *      ApprovedExecuted - Proposal votingPeriod has closed and vote passed. Proposal was successfully executed.
     *      QuorumNotMet - Proposal votingPeriod has closed and votingQuorumPercent was not met. Proposal will not be executed.
     *      ApprovedExecutionFailed - Proposal vote passed, but transaction execution failed.
     *      Evaluating - Proposal vote passed, and evaluateProposalOutcome function is currently running.
     *          This status is transiently used inside that function to prevent re-entrancy.
     *      Vetoed - Proposal was vetoed by   bytes(_description).length > 0 && bytes(_description).length <= maxDescriptionLength,
            "Governance: _description length must be between 1 and maxDescriptionLength"
        );

        // set proposalId
        uint256 newProposalId = lastProposalId.add(1);

        // Store new Proposal obj in proposals mapping
        proposals[newProposalId] = Proposal({
            proposalId: newProposalId,
            proposer: proposer,
            submissionBlockNumber: block.number,


the governance system to do required work in a generic way.
     * @param _targetContractAddress - address of registry proxy contract to execute transaction on
     * @param _callValue - amount of wei if a token transfer is involved
     * @param _functionSignature - function signature of the function to be executed if proposal is successful
     * @param _callData - encoded value(s) to call function with if proposal is successful
     */
    function _executeTransaction(
        address _targetContractAddress,
        uint256 _callValue,
        string memory _functionSignature,
       bytes memory _callData
    ) internal returns (bool success, bytes memory returnData)
    {
        bytes memory encodedCallData = abi.encodePacked(
            bytes4(keccak256(bytes(_functionSignature))),
            _callData
        );
        (success, returnData) = (
            // solium-disable-next-line security/no-call-value
            _targetContractAddress.call.value(_callValue)(encodedCallData)
        );

        return (success, returnData);
    }

    function _increaseVoteMagnitudeYes(uint256 _proposalId, uint256


   log(2^256/(10^27*100))/log(1.07) ~= 1635
     */
    function _quorumMet(Proposal memory proposal, Staking stakingContract)
    internal view returns (bool)
    {
        uint256 participation = (
            (proposal.voteMagnitudeYes + proposal.voteMagnitudeNo)
            .mul(100)
            .div(stakingContract.totalStakedAt(proposal.submissionBlockNumber))
        );
        return participation >= votingQuorumPercent;
    }

    // ========================================= Private Functions =========================================

    function _requireStakingAddressIsSet() private view {
        require(
 — we have a pretty close-knit group, and Matt and his wife, Katie, are very respected people,” said Flames veteran Michael Cammalleri. “I don’t really know what to say other than it’s tragic news for all of us, and we have been and continue to do our best to support them in whatever they need, and we’re there for them any way we can be.

“For us, (hockey and life) are intertwined. You make these relationships through hockey and have these great friendships. It’s really hard to see a friend go through something like that. It’s tragic.

“I know a lot of people in this world go through tragedy and have friends who have dealt with tragedy. All we can do is support them.”


and they became good friends. Lee even helped Hakesh create the much-needed special technique in order for him to be deemed an Airbending Master.. Vanca is Hakesh's cousin and Lee immediately fell in love with the first chick who would even talk to him. He managed to whoo her rather quickly. Out of fear of Lee's loudmouthed spiritual envoy, they married in order to avoid punishment for their intimacy. He's a great fighter having trained with fans, staves, dao swords, and Airbending forms (Ba Gua).

Hakesh: 18. A talented musician and - only by extension -a multi-talented airbender. More than anything, Hakesh enjoys using Airbending to become a one-man orchestra and even went so far as to create a technique to distort sound waves to fine-tune the pitches heLast Update

Judge
Charles A. Abdella

Trial Judge

Massachusetts District Court

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A Trial Judge of the Massachusetts District Court, he served as First Justice of the Western Worcester District Court, an Attorney in private practice, a Worcester Assistant City Solicitor and a civil law mediator.He is an active alumnus of the College of the Holy Cross being a member of the


'.$row->name.'</li>';
    $count++;
}

may do what you require. Your controller would be:
foreach($this->input->post(checks1) as $key => $value)
{
    echo $key . ' - ' . $value;
}

<author>Author1</author>
    <date>12/34/5678</date>
  </data>

  <data>
    <title>Title2</title>
    <description>Description2</description>
    <myid>2</myid>
    <author>Author2</author>
    <date>87/65/4321</date>
  </data>
</catalog>

A:

I have been researching a bit and found a fairly similar question here:
http://forums.tizag.com/showthread.php?p=76699
Here is what I have come up with, this seems to be mostly working except that Firefox is not recognizing it as an xml file even though I have added the xml:output.
File1.xml (note line two, referencing our transformation):
<?xml version="1.0" encoding="ISO-8859-1"?>
<?xml-stylesheet type="text/xsl" href="merge.xsl"?>
<catalog>
  <data>
    <title>Title1</title>
    <description>Description1</description>
    <myid>1</myid>
  </data>

  <data>
    <title>Title2</title>
    <description>Description2</description>
    <myid>2</myid>
  </data>
</catalog>

File2.xml:
<?xml version="1.0" encoding="ISO-8859-1"?>
<catalog>
  <data>
    <author>Author1</author>
    <date>12/34/5678</date>


to bind to effector cells \[[@B4]-[@B6]\]. However, this approach cannot completely reduce circulating IgE, and cannot control the initial cascade of asthma pathogenesis. It is also known that IgG is present in the airway lumen and submucosa under normal conditions \[[@B7]\]. Although antigen-specific IgG is induced after antigen inhalation, its role in bronchial asthma remains unknown. OVA-specific IgG in rat-sera, such as IgG1 and IgG2a, is reported to increase on day 21 after OVA inhalation in asthmatic models induced by OVA \[[@B8]\]. Platts-Mills et al. demonstrated a progressive increase in specific serum IgG titers with extended exposure and a prevalence of Th2 cytokine-dependent IgG in cats and dogs \[[@B9]\]. Immunotherapy by allergen vaccination is reported to increase antigen-specific IgG titers in allergy patients \[[@B10]\], thus suggesting that antigen-specificmice instilled with PBS or anti-OVA IgG1 were analysed. Values inside histograms represent the percentage of MHC class II expression on lung-derived CD11c^+^APCs. The numbers on the histogram show mean value of each percentage of gated cells from three independent experiments.](1465-9921-12-42-3){#F3}

Anti-OVA IgG1 ameliorated the activation of BMDCs *in vitro*
------------------------------------------------------------

The optical density of T cells, as measured with a cell counting kit, increased by addition of OVA peptide in co-culture medium with BMDCs compared to negative control (OVA peptide free). AntiOVA-IgG1 group significantly decreased the T cells proliferation (Figure [4](#F4){ref-type="fig"}). It means that antiOVA-IgG1 prevented BMDCs from presenting antigens, resulting in decreasing the number of T cells.

![**Anti-OVA IgG1 inhibited the cell proliferation of BMDCs in vitro**. BMDCs (2 × 10^4^cells per well) were purified from WT mice and pulsed


Hands Up! (2003 film)

Hands Up! is a 2003 Chinese historical comedy film directed and written by Feng Xiaoning, and produced by Han Sanping. The film stars Pan Changjiang, Guo Da, and Liu Wei. It based on the Second Sino-Japanese War.

Cast
 Pan Changjiang as the Imperial Japanese Army officer.
 Guo Da as Uncle Guo, a Chinese farmer and underground traffic policeman.
 Liu Wei as "Me"/ Mother/ Grandmother. The narrator of the film.

Others
 Hu Xiaoguang as The Tycoon.
 Li Ming as The interpreter of the Tycoon
 Bao De as the captain of Eighth Route Army.
 Zhang Xiaoning as an Imperial Japanese Army soldier.
 Li Baoguo as an Imperial Japanese Army soldier.
 Zheng Jie as an Imperial Japanese Army soldier.
 Gang Qiang as an Imperial Japanese Army soldier.
 Liu Damao as5025 style knob, from the Estate Collection, features a transitional design tha...t can accent both traditional and modern décor. Featuring your choice of 19 small sized rosettes, this premium knob is... read more

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blend into a number of traditional and classic déc...or choices. Featuring your choice of 19 small sized rosettes, this premium knob is fully customizable to meet your... read more

Rustic, yet composed, Baldwin's 5023 style knob, from the Estate Collection, will make a subtle addition to any traditionally deco...rated home. Featuring your choice of 19 small sized rosettes, this premium knob is fully customizable to meet your... read more

Rustic, yet composed, Baldwin's 5023 style knob, from the Estate Collection, will make a subtle addition to any traditionally deco...rated home. Featuring your choice of 19 small sized rosettes, this premium knob is fully customizable to meet your... read more

A familiar traditional design, the Baldwin 5041 style knob, from the Estate Collection, is ideal for those looking for a simple, c...lassicQ:

Geometric interpretation of a quintic's roots as a pentagon?

According to this subsection, "Geometric interpretation of a cubic's roots", given,
$$F(x)=x^3+ax^2+bx+c=0$$
with three real roots, "...then the roots are the projection on the $x$-axis of the vertices $A, B, C$ of an equilateral triangle. The center of the triangle has the same abscissa as the inflection point."
$\hskip2.7in$
$$\text{Fig.1}$$
Questions:

In general, given $F(x) = 0$ of degree $n>2$ with $n$ real roots, are the roots the projection on the $x$-axis of the vertices of a regular n-gon?
If so (and at least for deg $n=4,5$), what features of the $n$-gon (like $\theta$, center's abscissa, etc) can be given a closed-form in terms of the roots?

A:

Polynomials aside, this question is about how arbitrary sets of $n$ points on a line (namely, the $x$-axis) relate


"Gimme that." "Yes?" "' Get up!" "Miller my name." "Goethe High." "I have to visit a student intern." "Last name?" " Miller." "The student's." " No idea." "Chantal!" "Where are you?" "Trunk!" " How's it going?" "OK." "That bitch from Schiller private school is here too." "She's constantly dissing me, Mr. Miller!" " Well..." "Is this your social worker?" "Watch your mouth, baby tits." "How dare you?" " I'd like coffee too." "It's only for customers." "Whatever." "I take mine without gum anyway." "Oh, God!" "Do you at least get to test drive?" "Please, let's not put ideas into her head." "Chantal, you may continue cleaning the floor mats." "I simply can't imagine that anyone would hire her." "Mr. Miller, we're taking a golfie." "Blah blah." "We both"Fucking job, handing in a new form every day." "If you would invest only 50% of your criminal energy into lesson prep." "You didn't mention teaching was so much work." " Yes." "Getting up early!" "Here we go again!" "Another depressive episode, like the therapist said." "No." " Come on." "No!" "'Yes!" "I'm not sure if this one should be lower." "Grotesque." "I'd rather own a bar." " You can't afford a bar." "You'll work until 10B graduates..." "or at least tries to." "That could take decades." "I asked, you'd get a loan." "We've discussed it." "You can quit." "But only when you've saved up enough money to build a life." "It's important to me." "You need to learn to finish things, or you'll end up back in


time." "It's Kaiser, Mr. Miller's probation officer." "Probation's almost over." "We need to confirm Mr. Miller's address." "Schnabelstedt." "The truth in 5 seconds, or I've lied the last time for you." "Hello?" "You can tell your probation officer you forged the certificate of conduct." "Still there?" " Hello Mr. Kaiser." "I've been meaning to call." "About Mr. Miller." "Hey." "I wanted to surprise you" " Hello?" "With an international class trip." "So Ms. Gerster beats the Schiller school and you get the teacher campaign." "Ms. Schnabelstedt?" " Mr. Kaiser." "Excuse me." "Yes, Mr. Miller still lives here." " I see." "Perfect." "I'll tell him." "Bye bye." " Very good, bye." "I thought we could pay at least one teacher ticket, to keep the school budget down." "I should"Ms. Schnabelstedt is taking over the eco group." "She and Mr. Miller are going to?" " Thailand." "Ban Nam." "Thailand?" "Why?" "The Schiller school is already going there." "It's a bad idea." "It's cheaper than any other place." "They're used to student groups, they go there all the time." "And maybe we can steal their partner school." "That's the spirit!" "I have to take a few of my students along " "Chantal and them." " What?" "Elisabeth, you won't survive." " And a disabled student, please." "That too?" "But then it's a special ed trip." "Why?" "Because we're an inclusion school." "No backtalk." "Ask um," " Ploppi?" " Exactly." "He's all we've got." "You can't just change the whole group without asking us." "Yeah, yeah." "Go!" "He's so


"They screamed his locker, found a photo altar." "Screened." " Screamed his locker" "Not screamed... screened!" "You shit-for-brains." "What?" " But we will graduate with you, won't we?" "What did we learn today?" "Photosynthesis is not shit, it's hot shit." "Because plants are awesome!" "Click 'biology is awesome' and try my mega brain channel for study tips." "Over and out, your Hauke!" "Friend request Confirm" "Hey, Honey, look forward to seeing you on the class trip." "I can't go." "But I'd like to spend some time with you." "Don't tell anyone, but sometimes I secretly imagine kissing you." "No, I can't go." "But I have to go." "Yes." "Yes." "Father?" "Could you get down the big suitcase?" "We're twins!" "They let their folks bring them." "Embarrassing." " Bye,you care about our mascot anyway?" "Don't question me!" "It's annoying." "He's going psycho." "I'm not going psycho." "No, not there, it tickles." "Mr. Miller, I walked all the way back." "But I couldn't find your mobile, sorry." "Shitty camp." "Why did we come at all, if he didn't even feel like it?" "She bit me, that whore!" "I'm not a whore!" "Quiet or I'll smack you!" "Calm down!" "Ooh, cool nails." "Where's the mascot?" "OK." "She's not talking." "OK, time for waterboarding." " Hold her hands!" "Enough, she wants to talk." "You're sick!" "She wants more." "No, no, no." "My mobile!" "No." "I'll tell you where it is, you cunts." "Tsk, so abusive suddenly." "Go!" "OK." "Mr. Miller fucked up." "If my sister finds out, she'll flip."


me out." "Martial arts, eh?" " Yeah, a little." "Spent time on the streets as a kid, right?" "I saw you hanging out with the homeless kids." "Be careful." "They're trouble." "So?" "My students are too." "Why not help them instead of building solar bungalows?" "It's our partner school." "How we help them is our decision." "Your student's smoking near the pump." "Chantal!" "Get the flame closer to the pump." "Closer!" "Even closer!" "Oh, Mr. Miller, you're such a prankster." "Small, sad world of Goethe High." "1, 2, 3,4." "Statistically, the most common mobile phone PIN." "Diamonds?" "Forged documents?" "Best to delete text messages like that." "What do you want?" " The question is what don't we want, Mr. mini Ripley." "We don't want Gerster to report youa father to me." "One I could have sex with too, if he insisted." "Eeuw." "Chanti, you're so disgusting." "OK." "Can you imagine that what you got from your parents, you could get from everyone?" "You give something and you get something back." "Now go Pl3Y" "No, no, no!" "Wait!" "Man, it's going to drain all the dye out of my hair." "You're gorgeous." " Don't move." "What're you doing?" "Don't fuck around." " The princess needs her crown." "What's happening, Ploppi?" "The ring thing doesn't work, you get that, right?" "Magic's been dead since the middle ages." "When you do that, it looks crazy." "I have Asperger's. 11 percent." "I have vodka. 40 percent." "You don't like to be touched, huh?" " No." "Oh, man, don't smudge


my make-up, you freak!" "It's just color pigments and oil." "Paraffins." "Maybe a formaldehyde splitter." "Paraffinder?" "Petroleum." "Almost everything's made of petroleum." "Fertilizer." "Chemicals." "Plastic." "What!" "?" "That's totally gross." "Petroleum used to be this." "Algae and crustaceans." "They decay and become oil millions of years later." "Don't say that." " Yes." "Mr. Miller doesn't mean it that way." "He wants us to grow... up out..." "To grow out of it." " Exactly." "Mr. Miller is an asshole." " Yeah, but all teachers are assholes." "Better a cool asshole." " Ms. Schnabelstedt deserves better." "What's she look like naked?" "Is she prettier than me?" "I don't want to hurt either one of you." "Maybe you only pretend to like her, since you can't be with her anyway." "She'strip!" "Louder!" "Group Asshole!" "We're building an orphanage and saving the tsunami-assed little beggars." "If you do well, you'll get a real animal name." "I can take that." "Hello followers!" "it's Chanti." "A special hello to everyone from YouTube Bibi." "There are 3, 3 things to remember if you want to be a real Thai." "What are you doing, girl?" "Girl, I'm making a video." " Is that for real?" "Number 1, the greeting." "Danger!" "Hey, Chanti, what's up?" "No, Danger." "The Thais never shake hands." "They make a fin." "He's so bad." "We should drop him." "Number 2." "Even if you're loud, a Thai is never loud back." "Hey, piss face." "Whore." "Tramp." "Drop it, Chantal, it's annoying." "You said you'd help with my YouTube channel." "Nobody


now." "Almost as important as you are." "How many diamonds do you have left?" "One for each ear." "Hey, I got you the campaign." "That was the job." "Next time, the North Sea, you bum." "Um, Etienne?" "There was a mix-up with the chat recently do you have any questions?" "Mr. Miller told me that you maybe think I'm nicer than other women." "I love" "Etienne, that's sweet, but - loved you, but not anymore." "Excuse me?" "!" "I mean, good." "That's good." "But what did I do is it maybe because you saw me in a bikini?" "Efienne?" "I'm ready for reality." "'L'll never forget our first night'." "I'm the only one boinking you voluntarily." "I can do a whole 5 seconds." "Chantal?" "You can ride withis burning" "Action in the staff toilet" "Does no leg work!" "We've got the camera." "It's a higgledy-piggledy." "No sound, right?" "If this isn't cleaned up in 5 minutes" "Ouch!" "NO ANIMALS WERE HARMED IN THE MAKING OF THIS FILM." "ONLY ACTORS." "Yes, you can speak German?" " A little bit." "Why didn't you say so?" "We could've spoken German." "Must be finished by tomorrow!" "Schiller and Kamuz!" "Shut your face!" "Yes." "Should I answer it again?" "Well?" " Yes, answer it again." "It's always the scenes with French fries." "Shit." "Shit." "OK." "And to Thailand!" "I have to go there." "You're so passionate about your job." "That look was unbelievable." "I have to go there." "You crossed your eyes." " I did not cross my eyes." "


ice cubes?" "To Earth!" "Is it true that the shit" "Is it true that the shit from" "Is it true that the shit is thrown from Earth as ice cubes to Earth." "Is it true that the shit" "Is it true that the shit is thrown from ice cubes as Earth" "Is it true that the shit is thrown from the ice cubes" "What, man?" "50"')!" "" "I'll take Lisi's credit card and book a flight." "Excuse me!" " Gesundheit!" "No." "I'm definitely not going with those misfits" "You started that time, I saw it." "Stop it." "I'd go somewhere if Mr. Miller comes too." " That wasn't me." "How about Rome?" "I'd like to go back to Rome." "The wine, the light, the, the, the yada, yada."left. See Figure 1.0. The gene is highly conserved in vertebrates, including portions of the approximately 3,000 base pairs of 3'UTR.

Tissue distribution

Expressed sequence tag mapping of TMEM229B gene expression indicates that it is ubiquitously expressed throughout the body. TMEM229B is more heavily expressed in the parathyroid, skin, and thyroid tissues, and moderately expressed in bone marrow, trachea, spleen, eye, brain, pancreas, mammary gland, intestine, liver, thymus, lymph node, ovarian, muscle, lung, blood, and kidney tissues.

Protein

The translated TMEM229B protein is a total of 167 amino acids long, with a predicted molecular weight of 19,531 Daltons. The TMEM229B protein contains a domain of unknown function, part of the domain family DUF1113, spanning from amino acids 87 to 135.

Based on predicted structure TMEM229b is highly resemblant of a connexin


clip bears against the storm window. U.S. Pat. No. 3,760,541 to Fulcher discloses a conical shaped protector. U.S. Pat. No. 2,583,303 to Oltz discloses a fastener defined as a solid triangular shape which creates a U-shaped channel for a window for storm shutter. U.S. Pat. No. 4,074,483 to Vickstrom discloses a storm window system. U.S. Pat. No. 3,824,753 to Anderson discloses a window structure defining a U-shaped channel with a plurality of inboard directed lips inboard of the U-shaped channel. U.S. Pat. No. 5,123,211 to Schlicht et al. discloses a garage door light incorporating a uniquely shaped clip which is placed in a complementary shaped channel in the casement of the window. U.S. Pat. No. 4,179,859 to Fricko et al. discloses a molding retaining clip for an automobile.as a cooperative oncogene in *v-abl*-induced murine leukemia in 2002 \[[@R16]\]. Also discussed are potential applications of targeting specific AHI-1 interaction complexes as a new therapeutic strategy for treatment of CML and brain disorders.

IDENTIFICATION OF *Ahi-1/AHI-1* GENE BY PROVIRUS INSERTIONAL MUTAGENESIS IN MURINE LEUKEMIA AND LYMPHOMAS {#s2}
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Identification of the *Ahi-1* gene in *v-abl-*induced mouse pre-B cell lymphoma {#s2_1}
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The Ahi-1 (Abelson helper integration site-1) locus was initially identified as a common helper provirus integration site in 16% of Abelson murine leukemia virus (A-MuLV)-induced pre-B lymphomas (Figure [1](#F1){ref-type="fig"}) \[[@R28]\]. A-MuLV is a replication-defective murine retrovirus containing the *v-abl* oncogene which is responsible for its transforming potential \[[@R29]-[@R33]\] in murine models of leukemia and lymphomas. A-MuLV requires a non-defective helper MuLV virus to be able to replicate both *in vitro* and


in CTCL.

![Biological effects of *AHI-1* suppression in Hut78 cells and identification of its potential cooperating genes\
(A) The number of CFCs able to make colonies in the absence of any growth factor (-GF) is significantly lower in Hut78 cells with *AHI-1* suppression (AHI-1/sh4) compared to the empty vector control (RPG). This effect is rescued by adding the three growth factors (IL-2, IL-4 and TNF-α) to the media. (B) Subcutaneous injection of NOD/SCID-β2m immunodeficient mice with Hut78 cells and empty vector controls (Hut78/RPG) results in tumor formation within 4 weeks in all injected mice. *AHI-1*-suppressed Hut78 cells cannot form any local tumors in these mice even after 20 weeks. (C) Venn diagram of differentially expressed genes from the microarray analysis selected by both Limma and dChip analyses. Affymetrix GeneChip microarraywith TKI and JAK2 inhibitors\
(A) Detection of increased transcript levels of *AHI-1* and *BCR-ABL* in a CML stem cell-enriched population (lin^−^CD34^+^CD38^−^), progenitor cells (lin^−^CD34^+^CD38^+^) and their differentiated cells (lin^+^CD34^−^) as compared to purified normal bone marrow cells using Q-RT-PCR. (B) Overexpression of *Ahi-1* induces a lethal leukemia *in vivo* and enhances the effects of *BCR-ABL*. Survival curves of NOD/SCID-β2M^−/−^ mice injected with BaF3 cells transduced with control vector, *Ahi-1*, *BCR-ABL* and *Ahi-1* plus *BCR-ABL* (B/A). (C) Inhibition of colony formation in semi-solid media by Imatinib in lin^−^CD34^+^ CML progenitor cells from IM-responders, non-responders and blast crisis patient samples transduced with either a control vector or AHI-1/sh4 vector with suppression of *AHI-1*. (D) Schematic diagram of the AHI-1-BCR-ABL-JAK2 interaction complex that regulates constitutive activation of BCR-ABL and JAK2/STAT5 and