adjudged from our previous work \[[@B3]-[@B8]\], were compared for their influence on TsoI activity: SAM, a natural and obligatory co-substrate for MTase activity and an allosteric stimulator for *Thermus* sp. REase activities; SIN, a SAM analogue, which apparently causes subtle changes in tertiary *Thermus* sp. REases structures, either stimulating DNA cleavage \[[@B6]-[@B8]\] and/or causing substrate specificity changes towards much more frequent cleavage \[[@B5]\]; (BMC Genomics, in press) and S-adenosylhomocysteines (SAH), the methylation reaction by-products. These results as well as TsoI digestions performed for a prolonged time under various enzyme: substrate molar ratios show that the activation of REase function by SAM, SIN and SAH is weak, but detectable (Figure [8](#F8){ref-type="fig"}). Nevertheless, this may lead to indirect conclusion that the enzyme retained some capability for allosteric interaction of the TsoIlane 7, pH 7.0; lane 8, 7.5; lane 9, 8.0; lane 10, 8.5; lane 11, 9.0; lane 12, 9.5; lane 13, 10.0; lane 14, 10.5. **(C)** The influence of ionic strength on TsoI REase activity. 0.3 μg (= 0.6 pmol recognition sites) single site PCR substrate (390 bp) was digested with 0.33 pmol TsoI (0.016 u) in the NaCl concentration range from 0 to 60 mM for 30 min at 55°C in the standard reaction buffer, devoid of initial NaCl content: 10 mM Tris--HCl, pH 7.5, 10 mM MgCl~2~, 0.01 mg/ml BSA, 0.5 mM DTT, 50 μM SAM. Lane M, GeneRuler^TM^ 100 bp Plus DNA Ladder (Thermo Fisher Scientific (Fermentas); selected bands marked); lane K, undigested PCR fragment; lanes 1--7, digested PCR fragment: lane 1, without NaCl; lane


2, with 10 mM; lane 3, 20 mM; lane 4, 30 mM; lane 5, 40 mM; lane 6, 50 mM; lane 7, 60 mM.](1471-2199-14-17-9){#F9}

Conclusions
===========

i\. The prototype TsoI REase gene was cloned in *E. coli* and sequenced using a new modification of a classic biochemical selection method, where the positive selection vector was combined with two rounds of selection for the methylation phenotype.

ii\. Expression of a cloned *tsoIRM* gene under a T7 promoter, yielding enzymatically active bifunctional TsoI REase-MTase, required an additional round of the biochemical selection of expression subclones to eliminate abundant spontaneous mutants.

iii\. TsoI is a member of the *Thermus* sp. modular enzyme family and the TspDTI-subfamily, exhibiting a rare phenomenon among REases -- relatively high homologies to TspDTI, Tth111II and TthHB27I, even though they recognise distinctbefore induction and only partially protected after induction. Thus, considering the possibility of the appearance and selection for PCR errors in the large, toxic *tsoIRM* gene, the few expression constructs were subjected to DNA sequencing, which revealed the presence of multiple mutations. To select for mutation-free plasmids, 5000 ampicillin-resistant colonies obtained after the transformation of ER5266 by (pET21NS + *tsoIRM*-PCR fragment) ligation mixture were pooled and used to inoculate 100 ml LB supplemented with ampicillin. Cells were grown at 37°C until OD~600~ = 0.7, TsoI expression was induced by the addition of 1 mM IPTG. The culture was further grown at 37°C for 4 hours and then used for the isolation of total plasmid DNA. The latter has 7 TsoI targets and thus can be enriched for more


Q:

Finding certain values from a dictionary as an input to a constructor python

So I have the following code:
# constructor
def __init__(self, dict):

    # Inputs
    harvesting_time = 16 # hours
    recovery_rate = 90 # %
    unit_throughput = 93.63 # kg/annum/unit
    cost_per_unit = 275000 # $
    electricity = 0.0000331689825  # kWh/kg/unit
    energy_cost = 10.41  # cent/kWh
    labor_cost = 1777000  # $/annum
    concentration = 100 # g/L

    throughput = dict.get("output(kg/annum)",default=None)  # kg/annum
    carbon = dict.get("carbon",default=None)
    nitrogen = dict.get("nitrogen",default=None)
    carbohydrates = dict.get("carbohydrates",default=None)
    proteins = dict.get("proteins",default=None)
  September 21, 2013 - JP -

I have just pushed to Rubygems the new versions of Monologue and monologue-markdown.

Disclaimer: this blog isn't using Monologue anymore but Jekyll.

New to Monologue?

What is Monologue you may ask? Legitimate question! It is a barebone blog engine for Rails. It is built as a Rails engine and with a focus on keeping very few dependencies to ease it's use in your project. You can also use monologue-markdown which will replace the default editor with a simple Markdown editor.

For more information on how to use it, you can see the GitHub repo. Here is the branch for the current 0.3.X releases: https://github.com/jipiboily/monologue/tree/0-3-stable.

Give it a shot. Feel free to contribute, be it via a PR or simple feedback. Contributing will make you feel happy!

Already using Monologue?

Look


and help Michael with this. As usual, filing issues is welcomed and encouraged. PR are just awesome!

Communication pipelines

Feel free to ask questions on the mailing list: https://groups.google.com/forum/#!forum/monologue-rb. Feel free to get in touch with me (or anyone else) in the "new" IRC channel on Freenode: #monologue. As usual, GitHub issues are important. Let us know if you find bugs.

Project on GitHub is right here: https://github.com/jipiboily/monologue

One last thing...

I have a dream....well, a bunch, but one that is achievable. monologue-dashboard. It is nothing else than an idea of an extension that I would love to use but does not exist as of right now. I have outlined a few features that I would love for myself. You can see that in the README of the repo here: https://github.com/jipiboily/monologue-dashboard. Beers areShift work, long working hours and preterm birth: a systematic review and meta-analysis.
Specific physical activities or working conditions are suspected for increasing the risk of preterm birth (PTB). The aim of this meta-analysis is to review and summarize the pre-existing evidence on the effect of shift work or long working hours on the risk of PTB. We conducted a systematic search in MEDLINE and EMBASE (1990-2013) for observational and intervention studies with original data. We only included articles that met our specific criteria for language, exposure, outcome, data collection and original data that were of at least of moderate quality. The data of the included studies were pooled. Eight high-quality studies and eight moderate-quality studies were included in the meta-analysis. In these studies, no clear or statistically significant


to provide enhanced oversight and strategic direction for CASA; and
b. undertaking a review of CASA's funding arrangements to ensure CASA is equipped to deal with new regulatory challenges.
2. In accordance with the findings of the Hawke Taskforce, that CASA's Regulatory Reform Program be brought to a conclusion as quickly as possible to provide certainty to industry and to ensure CASA and industry are ready to address future safety challenges.
3. That the Australian National Audit Office audit CASA's implementation and administration of its Safety Management Systems approach.

Further incidents
Following the Lockhart River crash, Transair in Australia went into liquidation in late 2006. Aerotropics also no longer operates because the Civil Aviation Safety Authority cancelled its Air Operator Certificate due to ongoing safety breaches. Transair continued to operate its PNG businessuntil she retired. Despite being alone, she always had a positive outlook on life, she always joked that since she expected to die any day, every day was a surprise. I asked her how she liked her job at the bank. She replied it was a great job until the 1980s then things started to change. I asked, why?. Her answer surprised me, she said, that is when the WW2 veterans started to retire, even when they where in management they where never scared to question the bank. It was then that things started to go downhill.

As George MacDonald Fraser puts it, the WW2 veteran voted for Liberal which in those days meant labor.

With the exception of Parker, who I suspect voted Tory if he voted at all


                            <input type="text" id="FileField" name="FileField1"/>

Hope you can figure out the ascx page code difference.
ascx.cs Page code:

folder.Files.Add(folder.Url + "/" + fileUp.FileName, fileUp.FileBytes);
I want to change this line of code with the correct line of code which i am unable to do.
Please help.Thanks in advance

A:

Hi I got the answer for it.
Replace
folder.Files.Add(folder.Url + "/" + fileUp.FileName, fileUp.FileBytes);

to
string fileFieldPathValue = Request.Form["fileFieldPath"].ToString();
string fileName = System.IO.Path.GetFileName(fileFieldPathValue);
var bytes = System.IO.File.ReadAllBytes(fileFieldPathValue);
folder.Files.Add(folder.Url + "/" + fileName, bytes);

For me it worked perfectly.

of war and peace.

“There is no party in the United States, no democratic voice except third parties that are small, that would say ‘Why are we fighting wars?’” Stone exclaimed, adding, “it’s all right-wings fighting with right-wings.” Democrats are no better than Republicans, he says: “Hillary Clinton and her group, and Joe Biden, are just as pro-war as any Republican Dick Cheney.”

Stone thinks Donald Trump who has done “horrible things” by pulling out of the Paris climate accords and the hard-earned 2015 Iran nuclear deal. But, argued Stone, at least Trump was asking why the US needs to fight the Russians, which alarmed the mainstream so much that the media was attacking him from day one.

Also on rt.com It’s not Russia that’s damaging American democracy – it’s money

Stone


HOME FAST) do not have the capacity to be scored as 'NA' in the original HOME FAST, but were incorrectly indicated as NA in this pilot study. For instance, item 2 (floor coverings in good condition) had three NA ratings---that is, no floor exists, item 13 (accessible/sturdy grab rail/s in the shower or beside the bath) and item 14 (slip resistant mats/strips used in the bath/bathroom/shower recess) had four NA ratings---that is, no bath or shower exists, and item 23 (person currently wearing well-fitting slippers or shoes) had one NA rating---that is, the person has no feet. Item 5 (Can the person get in and out of bed easily and safely?) had one case of missing data, and item 12 (Is the person able to walk in andwith transfers and so on. This finding is important as many older people tend to believe that falls occur due to their own behaviour, rather than because of environmental factors, which may cause a low take-up of home hazard management options.[@R30] [@R31] It is possible that managing environmental falls risks may reduce the fear of falls.[@R32] This association can be explored in more detail in the larger MELoR study.

Although only gender and fear of falling were significantly associated with a higher number of hazards in this study, a larger study may reveal other factors associated with home hazards such as falls history, living situation and ethnicity. Even with a small sample, the study provides some initial direction about the characteristics of older people who are likely to have


wikipedia.org because it has the shortest web adress.

When i try to access it, in Internet explorer i get "cannot display the webpage" and in chrome there is "This site is not available".

What I've done

I have tried pinging wikipedia.org in cmd which works and i can ping it with 0% losses.

I have telnet wikipedia.org which don't work

I formated my OS HDD, reinstalled Windowps 7 ultimate 64

After that i formated my storage HDD

I downloaded Microsoft sec essentials from my laptop (more on this further down), and installed it on my PC with USB and ran it, nothing came up.

Did the same with SUPERAntiSpyware, and ran it on the computer. This found 19 items of tracking objects mostly accounts.google.com in my appdata directory.

I have been in contact with my ISP thatIn an iterative fashion we also identified partners of several Nanog-associated proteins (including Oct4), validated the functional relevance of selected newly identified components and constructed a protein interaction network. The network is highly enriched for nuclear factors that are individually critical for maintenance of the ES cell state and co-regulated on differentiation. The network is linked to multiple co-repressor pathways and is composed of numerous proteins whose encoding genes are putative direct transcriptional targets of its members. This tight protein network seems to function as a cellular module dedicated to pluripotency.


Functional analysis of conserved domains in the phosphotyrosyl phosphatase activator. Molecular cloning of the homologues from Drosophila melanogaster and Saccharomyces cerevisiae.
Phosphotyrosyl phosphatase activator (PTPA), a 37 kDa cytosolic protein that specifically activates the phosphotyrosyl phosphatase activity of the dimeric form of PP2A, was cloned from Drosophila melanogaster and Saccharomyces cerevisiae. Sequence alignment of PTPA from yeast to human revealed highly conserved regions including the type B fragment of the putative PTPA ATP binding site. We generated PTPA deletion mutants of these conserved regions as well as point mutations within regions that were suggested to be functionally important. The recombinant proteins were expressed in E. coli and subsequently purified. Activity measurements, linked with immunological detection, revealed that most of the well-conserved regions are essential for PTPA activity. However, neither* the remainder stores an array of ext4_extent.
 * For non-inode extent blocks, ext4_extent_tail
 * follows the array.
 */

/*
 * This is the extent tail on-disk structure.
 * All other extent structures are 12 bytes long.  It turns out that
 * block_size % 12 >= 4 for at least all powers of 2 greater than 512, which
 * covers all valid ext4 block sizes.  Therefore, this tail structure can be
 * crammed into the end of the block without having to rebalance the tree.
 */
struct ext4_extent_tail {
	__le32	et_checksum;	/* crc32c(uuid+inum+extent_block) */
};

/*
 * This is the extent on-disk structure.
 * It's used at the bottom of the tree.
 */
struct ext4_extent {
	__le32	ee_block;	/* first logical block extent covers */
	__le16	ee_len;		/* number of blocks covered by extent */
	__le16	ee_start_hi;	/* high 16 bits of physical


block */
	__le32	ee_start_lo;	/* low 32 bits of physical block */
};

/*
 * This is index on-disk structure.
 * It's used at all the levels except the bottom.
 */
struct ext4_extent_idx {
	__le32	ei_block;	/* index covers logical blocks from 'block' */
	__le32	ei_leaf_lo;	/* pointer to the physical block of the next *
				 * level. leaf or next index could be there */
	__le16	ei_leaf_hi;	/* high 16 bits of physical block */
	__u16	ei_unused;
};

/*
 * Each block (leaves and indexes), even inode-stored has header.
 */
struct ext4_extent_header {
	__le16	eh_magic;	/* probably will support different formats */
	__le16	eh_entries;	/* number of valid entries */
	__le16	eh_max;		/* capacity of store in entries */
	__le16	eh_depth;	/* has tree real underlying blocks? */
	__le32	eh_generation;	/* generation of the tree */
};

#define EXT4_EXT_MAGIC		cpu_to_le16(0xf30a)
#define EXT4_MAX_EXTENT_DEPTH 5

#define EXT4_EXTENT_TAIL_OFFSET(hdr) \
	(sizeof(struct ext4_extent_header) + \
	 (sizeof(struct ext4_extent) * le16_to_cpu((hdr)->eh_max)))

static inline struct ext4_extent_tail *
find_ext4_extent_tail(struct ext4_extent_header *eh)
{
	return (struct ext4_extent_tail *)(((void *)eh) +
					   EXT4_EXTENT_TAIL_OFFSET(eh));
}

/*
error of supposing that the early progenitor of the whole Simian stock, including
man, was identical with, or even closely resembled any existing ape or monkey.

~ Charles Darwin(born:1809-02-12 died:1882-04-19 at age:73)

Creationists claim Darwin said that monkeys turned into humans. No matter how many times we
correct them, they repeat the lie. They know they are wrong, but they compulsively lie anyway.

Why Are there Monkeys?

If it could be demonstrated that any complex organ existed that could not possibly have been formed by numerous,
successive, slight modifications, my theory would absolutely break down. But I can find no such case.

~ Charles Darwin(born:1809-02-12 died:1882-04-19 at age:73)

This frank admission of where to look for the chink in the armour of evolutionary theory was like
blood in the water to the creationist sharks. They searched and searched


It just adds the puzzles where did the wizard
come from? Who made the wizard’s gown? Where did the wand come from? How does waving a wand create universes?
How does the wand know what the wizard wants to create? Does it have an ESP (Extra Sensory Perception)
interface? Where is the wizard before he creates space? What was he doing before he got into the creation business?
What is he doing now the creation is over and he has become undetectable? You have not explained anything. You have
just exploded the complexity of the problem.

How Creationists Cheat

Our strategy has been to change the subject, a bit so that we can get the issue of Intelligent Design, which
really means the reality of God, before the academic world and into the schools.

Creation of Life

To      public struct SID_AND_ATTRIBUTES
        {
            public IntPtr Sid;
            public uint Attributes;
        }

        [StructLayout(LayoutKind.Sequential)]
        public struct TOKEN_GROUPS
        {
            public int GroupCount;
            [MarshalAs(UnmanagedType.ByValArray, SizeConst = 1)]
            public SID_AND_ATTRIBUTES[] Groups;


The Canadian Press

ST. CATHARINES, Ont. - The charges against an Ontario police officer accused of shooting a fellow cop a year ago have been dropped, lawyers said Friday.

Det.-Sgt. Shane Donovan of the Niagara Regional Police had been charged with attempted murder, aggravated assault and assault with a weapon in relation to the incident, which sent his colleague Const. Nathan Parker to hospital with serious injuries.

The pair had been investigating a traffic collision in Pelham, Ont., on Nov. 29, 2018, when they became involved in an altercation.

Donovan's lawyer, Joanne Mulcahy, said the charges against her client were withdrawn on Friday because the prosecutor felt there was no reasonable chance of conviction.

“Det.-Sgt. Donovan thanks (Crown attorney) Ian Bulmer and the Ministry of the Attorney General for making the right decisionrecovering from nine bullet wounds, is shocked and shaken.”

Parker faces three assault charges in the same incident, laid by the Ontario Provincial Police two months after the SIU charged Donovan.

This report by The Canadian Press was first published Nov. 22, 2019.


succession at one rate and output samples are retrieved at another rate greater or lesser than the input rate, depending upon whether time expansion or time compression is desired.
Various techniques are shown in U.S. Pat. No. 3,786,195 of Schiffman, some of which employ a variable delay line, some of which utilize digital shift registers, and one of which utilizes a random access memory.
Apparatus for audio signal time compression and expansion employing a delay line is likewise shown in U.S. Pat. Nos. 3,594,513 and 3,480,737 of Greenberg et al.on the end user’s computer to run it. Users simply need a web browser and internet connectivity to access and use our platform. Antivirus software that is installed on the end user’s computer will not have any compatibility issues with our platform as long as one of our supported web browser versions is used to access the system.


Co Meath.

Ms Sheehy said in an affidavit that Donaghpatrick had been home to her sister and herself for some 75 years and they had a small landholding with grazing for about 20 beef cattle. She has “grave concerns” about pylons near her home and feared this “obtrusive” development would destroy “not only my life but the life of my community and the simple rural landscape”.

Third case

The case is the third initiated in the High Court over the interconnector, which is due to run from Batterstown in Co Meath to Turleenan in Co Tyrone.

Earlier this month, David Malone, of Eurolaw Environmental Consultants, St Joseph Terrace, Portarlington, got leave to bring separate proceedings. A decision on whether to grant leave to another man to bring a third, also separate, case,junior residents with 1--2 years of experience in paediatric residency training, senior residents with 3--5 years of experience), managerial nurse, nurse and other. We calculated mean scores for each job role. Demographic data on assessors were analysed using hierarchical regression to calculate potential influences on assessees' ratings. This was undertaken with controls for the seniority of assessees (≥5 years and \<5 years), as it was accepted that performance would be affected by training. Other characteristics included assessors' gender, occupation, length of working relationship with assessees, educational background and year of graduation. P values (p\<0.01) were reported as a measure of the relative importance of each potential confounder.

### Reliability {#s2a11}

Reliability can be assessed in several ways including internal consistency with Cronbach\'s α coefficients and test-retest reliability, considered as classical test theory. Generalisability theory[@R14]


approval was obtained on 18 October 2012 from the independent review board of INTACT (UMIN000007064) which has its administrative office in Tokyo Women\'s Medical University.

**Provenance and peer review:** Not commissioned; externally peer reviewed.

**Data sharing statement:** No additional data are available.
Blunt Talk: Walter Tries to Be a Journalist Again in Season 2

The newsroom satire Blunt Talk returns for Season 2 with bombastic anchor Walter Blunt (Patrick Stewart, above right, with Erik Griffin) befuddled by his skyrocketing ratings. Last season, his program was a borderline bust, but after two shocking events—Walter passed out cold during one live telecast and was taken hostage during another—the show is now considered train-wreck television and an absolute must-see. In fact, it’s so hot there’s even an X-rated takeoff with a title not suitable for delicate ears!

Watch the exclusive clip about the Women of Blunt Talk:

“Walter would like to think his show is No. 1 because he’s doing such a great job reporting the news,” says executive producer Jonathan Ames. “But he starts to


When allowed to grow free in medium, bacteria grow in a logistic fashion; they can undergo a lag phase with little to no growth, then they grow exponentially until the environmental capacity limits growth, at which point the bacteria reach stationary phase ([@bib24]; Zwietering *et al.* 1990; [@bib16]). Bacterial growth curves can thus be fit by a sigmoid defined by inoculum, maximal growth rate (r) and growth plateau (K) ([@bib25]). This description of logistic growth is a common starting assumption with many mathematical models that describe host--microbe interactions in hosts (for examples, see [@bib1]; [@bib28]). Though growth within a host will obviously be more complicated than growth within medium because the host raises an immune response, we started with a simple logistic description as an entry-level model tosimple to introduce consistent doses into the fly, 2) it is simple to measure growth, and 3) we already know that the fly varies in its response to *L. monocytogenes* in both resistance and tolerance ([@bib4]); thus this microbe seemed like a useful tool to probe variation in host immunity.

Natural variation affects immunity, and in *D. melanogaster* changes in bacterial growth properties are associated with natural variation of immunity genes ([@bib19], [@bib20]; [@bib13]; [@bib15]; [@bib30]). The effect of the fly genotype on median bacterial load is dependent on the bacterial pathogen such that a given host genotype does not have a universal effect on a range of bacterial pathogens ([@bib20]). Collections of genetically diverse *D. melanogaster* lines have been compiled to exploit natural genetic diversity to find the determinants of complex


traits. One such source is the Drosophila Genomic Reference Panel (DGRP), a collection of 205 homozygous naturally derived *D. melanogaster* lines with diverse nucleotide polymorphisms ([@bib21]; [@bib32]). This resource has been used to find genetic determinants of immunity, for example, the nature of *Drosophila* resistance for two natural *D. melanogaster* viruses (DCV and DMelSV) and two viruses from other insects (FHV and DAffSV) ([@bib22]). We used this host variation to test how host genetics change pathogen growth dynamics.

We leveraged genetic variation in the host, using both natural variants from the DGRP as well as known *D. melanogaster* mutants, to evaluate the phenotypic range of bacterial growth during an infection. By infecting *D. melanogaster* with a known initial dose of *L. monocytogenes*, and measuring bacterial growth over the course of infection, we foundhomogenized in 200 μl, 100 μl was plated, followed by 10 μl spots of 1:10 serial dilutions (from the remaining 100 μl) in order to capture the full range of infection. When injecting 1000 or more bacteria, flies were homogenized in 200 μl, and 10 μl spots of 1:10 serial dilutions were plated. At least eight samples were counted to determine the median number of bacteria for each inoculum. Plates were then incubated overnight at 37° before counting.

Survival curve analysis {#s6}
-----------------------

Sixty flies per line were injected and checked daily to measure mortality for each inoculum (PBS, 10^1^, 10^2^, 10^3^, 10^4^, 10^5^). Flies were housed in vials containing 20--25 flies each.

Repetitions {#s7}
-----------

Each set of conditions was repeated at least three times; for example, an experiment for a mutant fly


70 flies from each DGRP strain. We measured survival rates by recording median time to death (MTD), and measured bacterial loads at 48 hr postinfection by plating out homogenized flies. We found that these naturally varying lines exhibited a nine-fold range in MTD (from 1 to 9 days; [Figure 1A](#fig1){ref-type="fig"} and Supporting Information, [Table S1](http://www.g3journal.org/lookup/suppl/doi:10.1534/g3.115.022558/-/DC1/TableS1.pdf)) and an ∼350 fold range in bacterial load (from 6.44 × 10^3^ to 2.41 × 10^6^ CFUs; [Figure 1B](#fig1){ref-type="fig"} and [Table S2](http://www.g3journal.org/lookup/suppl/doi:10.1534/g3.115.022558/-/DC1/TableS2.pdf)). From these lines, we selected 11 for further study, choosing lines with a broad distribution of both survival rates and bacterial loads; these are not "the best" lines, rather they display a useful range of phenotypes ([Figure 1](#fig1){ref-type="fig"} colored columns and arrows, and [Table S3](http://www.g3journal.org/lookup/suppl/doi:10.1534/g3.115.022558/-/DC1/TableS3.pdf)).

![Median time to death (MTD) and bacterial load following *Listeria* infectionbe fit with three-parameter logistic curves that are defined by input load, maximal growth rate (r) and growth plateau (K) (A. Louie, personal communication). We anticipated that if growth of *L. monocytogenes* within the fly was really controlled by variation of r and K then we could observe this if we measured *L. monocytogenes* growth curves in these various fly lines. The logistic growth model predicts that when K is much larger than the input load of microbes, we would find that a logistic model provided a "good" fit for the data. However, as K dropped continuously to approach the input microbe load, bacterial growth would appear flat; under these conditions the variance in the system regarding microbe load would result in these low K lines having a less good


the w^1118^ parental line and two *piggybac*-insertion mutant fly lines, *[kenny](http://flybase.org/reports/FBgn0041205.html)*, a gene in the IMD signaling pathway, and the *[CG2247](http://flybase.org/reports/FBgn0030320.html)*, a gene necessary for the melanization immune response (A. Louie, personal communication). Mutations in these two genes affected the dynamics of bacterial growth; the growth plateau of both mutants was significantly greater than the parental control (w^1118^), and the growth plateau of *[kenny](http://flybase.org/reports/FBgn0041205.html)* was even higher than that of *[CG2247](http://flybase.org/reports/FBgn0030320.html)* (plateau from *[kenny](http://flybase.org/reports/FBgn0041205.html)* was 4.5-fold larger than that of *[CG2247](http://flybase.org/reports/FBgn0030320.html)*, and *[CG2247](http://flybase.org/reports/FBgn0030320.html)* was 60-fold larger than the plateau from w^1118^, *P* \< 0.05). This increase in growth plateau was not accompanied by an increase in bacterial growth rate ([Figure 6](#fig6){ref-type="fig"}, [Figure S5](http://www.g3journal.org/lookup/suppl/doi:10.1534/g3.115.022558/-/DC1/FigureS5.jpg), [Figure S6](http://www.g3journal.org/lookup/suppl/doi:10.1534/g3.115.022558/-/DC1/FigureS6.jpg) and [Table 3](#t3){ref-type="table"}). To investigate the growth rates at a higher resolution we measured theYou are here

The Douglas Hospital Research Centre Is Unionizing

Montreal, March 13, 2018 – The Tribunal administratif du travail (administrative labour tribunal) has recently certified the Public Service Alliance of Canada (PSAC-Quebec) as the union representing the Research Centre of the Douglas Mental Health University Institute (Douglas RC). Readers will recall that on December 22, PSAC-Quebec filed an application for certification to represent all Douglas RC employees.

“We could tell this group was enthusiastic from the get-go. We are immensely proud of the outcome and welcome them into the PSAC-Quebec fold. Not to mention that unionizing will increase the strength of workers in hospital research centres,” noted Magali Picard, Regional Executive Vice-President of PSAC-Quebec.

This unit consists of approximately 300 research associates, research assistants, technicians, research coordinators, animal care attendants and


has been the Spanish press company Prisa, whose conservative daily El Pais is also known for its inventive coverage of their former colony.

But stupid mistakes like this are never directly politically motivated. It was only coincidence that after a rightwing radio host Jorge Melgar Quete was arrested for calling on air for Evo to be assassinated that the Fox News of Bolivia, Unitel aired this months old tape of government minister Ramon Quintana at a pre-August 10th Recall Referendum rally in Pando calling for voters to "politically bury [Pando Prefect] Leopold Fernadez" in the upcoming vote, which is obviously an equivalent call to violence and somehow implicates the government in the September 11th massacre of 30 campesinos by Fernadez's paramilitary goons. And its only fair and balanced thatin office anniversary speech after the uppity Indian had the nerve to tell the truth to his face;

“The United States has fomented the regional disintegration of the country, holding secret meetings to promote disturbances against the national government.”

Urs parroted former US Ambassador douchebag Goldberg telling journalists the statements are “unfounded and false.”

Perhaps, except for that secret meeting Goldberg had with those secessionists and fascists prior to an attempted violent coup, and except for the US Embassy's internal documents showing USAID staff trying to organize political opposition to Morales. But you have never heard about any of that because everything I have just told you has been censored from US press coverage of Bolivia.

If Obama wants to show the world that "America is a friend of each nation and


fast down a country road in Santa Cruz, Bolivia, heading towards his sugar cane fields. He coaxes the red Mercedes over ninety and passes a truck full of peasants, regarding them in his rearview mirror.

"I bet they're headed to La Paz to take over the Congress or something," he says. "A new constitution is going to bring nothing but more problems."

From August 2006 to December 2007, Aguilera was actually part of an assembly that rewrote Bolivia's constitution. The draft will be approved or rejected by a highly anticipated referendum here on January 25.

In the morning on Sunday, January 18, after a heavy rain fell on La Paz, Bolivia, the sun came out, drying the umbrellas of thousands of marchers winding through the city streets. The mobilization was inknowledge in which Miss Laura Zuñiga has been involved," failing to demonstrate "adequate behavior, avoid scandals and bad habits and be a good example for society." Glad to see they are maintaining standards. This is all certainly a spectacle.

Tuesday, January 06, 2009

"Israel must change its policies and the U.S. government should not continue using a country like Israel to invade countries that are seeking liberation... The killings are not a solution in this new millennium, so Israel should stop being a tool of U.S. imperialism to humiliate the Palestinian people." (photo, The Angy Arab)


Quasiparticlelike peaks, kinks, and electron-phonon coupling at the (pi,0) regions in the CMR oxide La2-2x Sr1+2x Mn2 O7.
Using angle-resolved photoemission, we have observed sharp quasiparticlelike peaks in the prototypical layered manganite La(2-2x)Sr(1+2x)Mn(2)O(7) (x=0.36,0.38). We focus on the (pi,0) regions of k space and study their electronic scattering rates and dispersion kinks, uncovering bilayer-split bands, the critical energy scales, momentum scales, and strengths of the interactions that renormalize the electrons. To identify these bosons, we measured phonon dispersions in the energy range of the kink by inelastic neutron scattering, finding a good match in both energy and momentum to the oxygen bond-stretching phonons.Life Span Studies, will appear in early 1998 in the journal Brain Research.

Most scientists interested in diseases like Alzheimer's and Parkinson's, which stem from massive loss of brain cells, focus on drugs and surgery, Tessel said.

But Tessel's work is related to research at other universities suggesting that lifestyle can make a difference in the progression of such diseases.

"Several have hinted that the more education you have, the less likely you are to get Alzheimer's or Parkinson's," he said.

The root cause of Parkinson's is the lack of a chemical messenger, dopamine, that serves as a relay between certain nerve cells in the brain.

The relationship of Parkinson's to dopamine is underscored in the name of the drug commonly used to treat Parkinson's: L-dopa.

In their experiments, Tessel and Schroeder used four


Q:

PHP - MYSQL - Display Pages by id from One php file

I have a problem to do the code work...I don't know why its not working...I want to display from the database title and many other by id...The name of the file is view.php...If I want to show id 1 then I type on url view.php?id=1 or id 2 view.php?id=2...here is my code please help me out...
<?php
$getid = $_GET["id"];

$dbuser = 'user';
$userpass = 'pass';
$database = 'db';

error_reporting(0);
$con = mysql_connect("localhost",$dbuser,$userpass);
if(!$con){die('Could not connect:'.mysql_error());}
mysql_select_db($database, $con);
mysql_query("SET NAMES utf8");
date_default_timezone_set('Europe/Athens');

$query = mysql_query("SELECT id,title,description FROM player WHERE id = '$getid'");
if(mysql_num_rows($query)){
    $result = mysql_fetch_assoc($query);
    $id = $result['id'];
    $title = $result['title'];
    $description = $result['description'];
    echo 'ID: '.$id.'
Title: '.$title.'
Description: '.$description;
}else{
   a day's worth of interest, making the total accumulate to a value more than a dollar by tomorrow. Interest can be compared to rent. Just as rent is paid to a landlord by a tenant, without the ownership of the asset being transferred, interest is paid to a lender by a borrower who gains access to the money for a time before paying it back. By letting the borrower have access to the money, the lender has sacrificed the exchange value of this money, and is compensated for it in the form of interest. The initial amount of the borrowed funds (the present value) is less than the total amount of money paid to the lender.

Net present value

In finance, the net present value (NPV) or net present worth


guide!
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PC Power Supply Scrap or Sell

End of LifePower supply units don't sell for much as scrap with scrap metal being low I took a look at how much extra value can be pulled from a PSU, for lighter types there's not much value either way but the heavy switching type PSU's it's around double value for taking the time to scrap them out.
Check out my recommended safety products, Furnaces & stuff on amazon.. https://www.amazon.com/shop/ewasteben

11:42

What is inside a printer? What is the scrap value of it's contents? GOLD and steel and motors!

What is inside a printer? What is the scrap value of it's contents? GOLD and steel andan asset's salvage value is subtracted from its initial cost to determine the amount by which asset depreciated over useful scrap worth of a physical individual components when itself deemed no longer usable. Scrap value the strategic cfo. Accountingcoach accountingcoach blog what is scrap value url? Q webcache. How to calculate depreciation on fixed assets (with calculator). Googleusercontent search. Thus, salvage value 24 jul 2013 scrap value, defined also as is the of an asset after it fully depreciated 25 jan 2016 we need to update and reduce form book remaining amount start depreciation. The value is used in accounting to determine depreciation amounts and 1 jun 2015 scrap formula rate cost of fixed asset (rv lifespan) where, rv example consider 25, a process allocation. When it breaks


this video, I go over how I scrapped a brass bed I picked up along side the road for free. http://rover.ebay.com/rover/1/711-53200-19255-0/1?icep_ff3=11&pub=5575168947&toolid=10001&campid=5337864703&customid=&icep_store=terminal99&ipn=psmain&icep_vectorid=229466&kwid=902099&mtid=824&kw=lg - Link to my terminal99 eBay store! - Link to my terminal99 eBay store! - Link to my terminal99 eBay store!

Important Tips on Preparing Catalytic Converters for Scrap

http://iscrapapp.com - When you are looking to recycle or scrap your catalytic converter with your local scrap yard, Tom encourages you to follow these simple s...

http://iscrapapp.com - When you are looking to recycle or scrap your catalytic converter with your local scrap yard, Tom encourages you to follow these simple steps in bringing or sending the materials and items to your yard. If you are tempted to take the catalytic converter apart for the precious metals inside, it isn't thethe definition. Analyzing the definition of key term often provides more insight about concepts. Scrap value can be defined as: Estimated of amount to be recovered at the end of an asset’s useful life; also called salvage value or scrap value. Scrap value is a term that generally related to property plant and equipment, long term tangible assets, scrap value representing the left over, or residual value, at the end of an assets useful life.
Why Learn Accounting - Financial Accounting / Managerial Accounting
https://youtu.be/uaWDB1YdA1k?list=PL60SIT917rv52SlrB3FFn2WMyZEkj6uBI
101DoubleEntryAccounting System Explained - Accounting Equation
https://youtu.be/66e9QbrkE4g?list=PL60SIT917rv52SlrB3FFn2WMyZEkj6uBI
101 Cash vs Accrual - Cash...

published: 23 Feb 2017

How To Calculate the Value of Scrap Gold

How to Calculate the Value of Scrap GoldHow to videos for blind people.
If you have scrap gold, you can sell it. Gold prices tend to increase


a lot but all are good for a little gold recovery and other precious metals.
This Sanyo wasn't the best value LCD to start with but if your stacking circuit boards for gold recovery then it all adds up.
But if your depopulating circuit boards for refining yourself then values will be much higher, my prices are just for selling the boards and metals as is at current scrap metal prices.

published: 21 Nov 2015

Scrap Gold Get Cash For Gold - Sell Gold for cash - Scrap Gold Buyers

Get Cash For Gold - Sell Gold for cash - Scrap Gold Buyers
Everyone is talking about the amazing prices gold is getting in the market today. With gold values going through the roof, right now is the perfect time to sell your golddefinition & example what? Jbsclasses. For example, a firm's computer depreciates each year. Apr 2015 learn how an asset's salvage value is subtracted from its initial cost to determine the amount by which asset depreciated over useful scrap worth of a physical individual components when itself deemed no longer usable. Scrap value the strategic cfo. Accountingcoach accountingcoach blog what is scrap value url? Q webcache. How to calculate depreciation on fixed assets (with calculator). Googleusercontent search. Thus, salvage value 24 jul 2013 scrap value, defined also as is the of an asset after it fully depreciated 25 jan 2016 we need to update and reduce form book remaining amount start depreciation. The value is used in accounting to determine depreciation amounts and 1 jun 2015 scrap formula rate


value being ignored in depreciation calculation financial definition of dictionary. Cost to be allocated acquisition cot salvage value. What is scrap value? What Scrap value definition & example what? Jbsclasses. For example, a firm's computer depreciates each year. Apr 2015 learn how an asset's salvage value is subtracted from its initial cost to determine the amount by which asset depreciated over useful scrap worth of a physical individual components when itself deemed no longer usable. Scrap value the strategic cfo. Accountingcoach accountingcoach blog what is scrap value url? Q webcache. How to calculate depreciation on fixed assets (with calculator). Googleusercontent search. Thus, salvage value 24 jul 2013 scrap value, defined also as is the of an asset after it fully depreciated 25 jan 2016 we need to updatewhen 7 may 2016 at some point, book only represent scrap value, after all depreciation has been taken. In financial accounting, scra

Scrap Happy Daffy | World War 2 Era PropagandaCartoon | 1943 | News Todays
Welcome to News Todays. ● CHECK OUT OUR 2ND CHANNEL:
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Scrap Happy Daffy is a Looney Tunes black and white cartoon featuring Daffy Duck, directed by Frank Tashlin and released in 1943 by Warner Bros.Daffy is the guard of a scrap yard, doing his part to help America win the Second World War against the Germans, but the enemy decide to destroy his scrap pile by sending a billy goat out to eat everything in sight.
The short was also the final appearance of Daffy Duck in black and


the disproprotionate amount of questions I receive about buying gold and silver whenever gold and silver prices are rising significantly versus when gold and silver prices are falling. In other words, most people believe that that top reason they should buy gold and silver is to profit from rising prices. However, this is far from the best reason to buy physical gold and physical silver. The number one reason to buy physical gold and silver, bar none, is the global currency rot that is happening today, that is relentless, and that Central Bankers are now helpless to stop (though they are responsible for creating it). Of course, some may say that benefiting from rising fiat currency prices of gold and silver is the same reason as protecting onselfcurrent market value of gold.
Broken Gold
Broken gold watch or jewelry? No problem! Cash for Gold USA will buy your gold in any condition. The value of your materials is dependent only on the purity of the gold and the weight of the object.
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Cash for Gold USA will pay you top dollar for your old or broken watch. If you have an old or broken gold watch in a drawer, you can turn it into cash today with Cash for Gold USA. We will buy your watch regardless of its condition.
Scrap Gold
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Scrap gold is any old or unwanted object containing gold. This can include watches, broken necklaces and bracelets, rings, earrings, and gold dental scrap – pretty much anything that contains gold. Our refiners will separate


in birmingham jewellery quarter. With uncertain financial markets, scrap gold prices have risen. Scrap our prices are based on the live gold spot price, and updated every two minutes, ensuring that you receive a fair accurate rate for your scrap jewellery sell 24 carat. Scrap 9ct gold 9 carat scrap prices 24ct 24. Online scrap gold value calculator how much is my worth [usd] from dendritics. Gold price per gram eur ( ) bullionbypost. Calculate the real value of your scrap gold tradersgold price traders. Value of scrap gold in 4 steps!! calculator (live price) goldcalc. Unique opportunity to sell directly a global gold refinery. Scrap gold weight conversion and melt value calculator. Daily gold prices and scrap calculator. Scrap gold buyers daily prices & calculator metallix scrap.exceeds net book. Allocation method 29 apr 2013 salvage value is subtracted from the cost of a fixed asset to determine amount that will be depreciated. Allocated over the estimated useful life of assets. In this situation, scrap value is defined as the expected or estimated of asset at end its useful life. Scrap value is also referred to as an asset's salvage or residual scrap value, called the of asset after it has come end its useful life 17 jun 2015 depreciation owners these assets sell them and estimated they hope get for 20 calculate at 10. The 'scrap' or 'salvage' value of the item represents how much it will be worth declining balance methods depreciation, specifically double method, do not take into consideration salvage an asset


man toppled by America’s CIA in 1953 – Roger Cohen writes in the New York Review of Books that the West not only put Iran into a democratic death-spiral that led to the rise of theocratic rule. It also set a precedent that it clings to today, by overthrowing “troublemakers” and tolerating tyrants who keep the flow of oil to the West. And it is this tendency in US foreign policy that accounts for much of the hostility that fuels leaders like Iran’s current President Mahmoud Ahmedinejad, and others.Free Download APPS/Games For PC Windows 7/8/8.1/10/XP.Play Android Games on PC and Laptop Using Android emulator.Free Download APPS or Games For Windows 10, Windows 8,Windows 8.1, Windows 7, Windows Vista,MAC OS, and Windows XP.Best APPS/APK For Windows.Our games or apps are licensed Full Version for PC

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The biggest fancy dress party on mobiles – now featuring 10 spooky new characters to celebrate Halloween!

Create your own superhuman and cross paths with up to 150 other heroes & villains in the ultimate shared universe. Decide where your loyalties lie and fight for control of every corner of Super City as your own unique story plays out.

Inheriting an above-average combat system from the Wrestling Revolution series, this


/*
 * This program source code file is part of KiCad, a free EDA CAD application.
 *
 * Copyright (C) 2004-2012 Jean-Pierre Charras
 * Copyright (C) 2004-2020 KiCad Developers, see AUTHORS.txt for contributors.
 *
 * This program is free software; you can redistribute it and/or
 * modify it under the terms of the GNU General Public License
 * as published by the Free Software Foundation; either version 2
 * of the License, or (at your option) any later version.
 *
 * This program is distributed in the hope that it will be useful,
 * but WITHOUT ANY WARRANTY; without even the implied warranty of
 * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
 * GNU General Public License for more details.
 *
 * You should havetrafficking to neurons or incorporate it to the polymer glycogen (Magistretti et al. [1993](#phy213484-bib-0029){ref-type="ref"}; Pellerin et al. [1998](#phy213484-bib-0036){ref-type="ref"}). Astrocytes maintain the major glycogen depot in the mammalian brain (Phelps [1972](#phy213484-bib-0037){ref-type="ref"}; Koizumi [1974](#phy213484-bib-0025){ref-type="ref"}). Brain glycogen, although limited in mass, is the principal alternative to blood‐derived glucose as a source of energy. This energy reserve is dynamic during normal brain activity and metabolic stasis, and is an important source of lactate equivalents during states of heightened activity or glucose deficiency (Stobart and Anderson [2013](#phy213484-bib-0042){ref-type="ref"}).

Glucopenia poses a significant risk of neurological dysfunction and injury as a continuous glucose supply is required to maintain high‐energy nerve cell functions. Neuro‐metabolic stability is monitored in a small set of brain structures, including the mediobasal hypothalamus (MBH), where distinct populations of specialized neurons are capable of either


Mr Eidelson, who last week revealed the damage to the rat stencils, says he thinks the whole wall should be preserved and possibly displayed in the National Gallery of Victoria. The stencils sat at the top of the blocked entranceway before the new doorway was installed. The recent damage is the latest in a series of mishaps that have destroyed a number of works by the elusive street artist, who painted stencils in city laneways when he visited Melbourne in 2003. "There are another two Banksys in that lane, they are the best Banksys left in Australia," Mr Eidelson says. "Obviously that's an absolute [attraction], three local Banksys. "In 2003 when Banksy visited, that was a seminal moment in street art," he says, that was influential for anumber of Australian artists.

"The whole wall should have been put in the NGV." Detail of one of the Banksy stencils destroyed during construction of a doorway in AC/DC Lane. Mr Eidelson says the works should be protected in the same way as heritage sites and streetscapes. "Some things go beyond ephemera, its part of the national [culture]. They should be walled up, sealed off or removed to the NGV. Haha's stencil of Ned Kelly in Berlin has been seen all over Melbourne, and even in Berlin. Credit:Leigh Henningham

"Despite the whole ideological thing about graffiti, this is different. In 20-30 years time people will look back and say 'This is where it began'." Mr Eidelson questioned why the works were not protected before the building work began. "With these


Management Plan notes that street art is by its nature temporary, ephemeral and forever changing." He said the council had no plans to preserve the remaining Banksy works in the laneway. There are precedents for protecting street art and displaying works in public galleries: the original door from the Collingwood mural painted by US street artist Keith Haring in the 1980s now hangs in the NGV. It had been stolen from the site but was later returned to Arts Victoria. The National Gallery of Australian in Canberra also has a substantial collection of Australian street art. Geoff Newton, a Melbourne gallerist who also values cultural gifts (items of significance donated from private collections to public institutions) said there were several arguments about whether street art should be protected.

"Thewith the same genetic background were from Liver Cancer Institute of Fudan University (Shanghai, China) [@pone.0089219-Wang1], [@pone.0089219-Tian1]. All cell lines were from male HCC.

Reagents {#s2c}
--------

The iTRAQ™ Reagents Kit was from Applied Biosystems (USA). TSPY1 small hairpin (sh) RNA fragments were purchased from Genechem (Shanghai, China). Rabbit polyclonal to TSPY1 was from Abcam Company. Mouse monoclonal to Flag was purchased from Sigma. Taq polymerase purchased from TAKARA. Lipofectamine 2000 was purchased from Invitrogen. Dulbecco's modified Eagle's (DMEM) medium, Roswell Park Memorial Institute 1640(RPMI-1640) and fetal bovine serum (FBS) were from Sigma Group and Gibco Company.

Tissue Samples Collection and Protein Extraction {#s2d}
------------------------------------------------

All samples were obtained from the Department of Hepatobiliary Surgery, First Affiliated Hospital of Guangxi Medical University (Nanning, China). All the patients were from the same geographic area as


the normal group with the similar genetic background and lifestyle. The clinic pathological features of the samples were listed in [Table 1](#pone-0089219-t001){ref-type="table"}. All the participants were negative for antibodies against hepatitis C virus (HCV), hepatitis D virus (HDV), diabetes and hypertension. Statistical analysis showed no significant difference in age, the level of serum AFP, ALT, AST and albumin, tumor size, cirrhosis and tumor stage between the male and female HCC groups. The diagnosis of HCC was confirmed by histopathological examination, no patients had undergone radiotherapy and chemotherapy before surgery. Fresh tissues were rapidly frozen in liquid nitrogen after surgical resection, and later transferred to −80°C for preservation. After removing the blood and vessels, about 0.2 g tissue was slightly cut into pieces and 1 ml lysis buffer (20After incubation for 36 h, the non-migrating cells in the upper chamber were removed using a cotton swab and the cells that had migrated to the underside of the membrane were fixed with 4% paraformaldehyde for 30 min, stained with 10% Giemsa for 30 min. The cells in the underside of the membrane were counted under light microscopy.

Cell Apoptosis {#s2k}
--------------

Prepare enough cells for incubating with the MuseTM Annexin V&Dead Cell Reagents and the cells were resuspended in 1% BSA-PBS. Added 100 µl of MuseTM Annexin V& Dead Cell reagent to a new tube and then mixed with 100 µl of cells in suspension. The cells were incubated for 20 min at room temperature in dark and counted by MuseTM Annexin V& Dead Cell software.

Soft Agar Colonization {#s2l}
----------------------

First, 1


male hepatocarcinoma.

Validation of Differential Proteins in Clinical Samples {#s3c}
-------------------------------------------------------

In order to validate the iTRAQ results, four proteins out of the 73 differential proteins based sex-related disparity, i.e. TSPY1, AK1C1, B2L13 and CP27A were chosen for further validation in 38 male and 14 female HCC tissues. These proteins were selected according to the following criterion: (1) the big fold changes of differential expression between male and female HCC groups; (2) the biological behavior was strongly correlated with the occurrence and progression of cancer; (3) and its mechanism was unclear in HCC. The RT-PCR results indicated that the mRNA expression of those proteins were consistent with the iTRAQ results, TSPY1 and AK1C1 were increased, meanwhile B2L13 and CP27A were decreased in the male HCC tissues ([Figure 3](#pone-0089219-g003){ref-type="fig"}). Furthermore, after paidthe static form $$ds^2=\frac{e^{2\gamma}}{f}[d{\lambda}^2+d{\mu}^2]+
\frac{{\rho}^2}{f}{d{\phi}^{\prime}}^2-f{dt^{\prime}}^2
\label{c4}$$ with the same functions $\gamma$ and $f$ given in (\[elbe\]). This is done by performing the transformation: $$dt=Adt^{\prime}+Bd{\phi}^{\prime}\;\;,\;\;
d\phi=Cdt^{\prime}+Dd{\phi}^{\prime},
\label{c5}$$ where $A,B,C,D$ are functions of the non ignorable coordinates $\lambda, \mu$.\
Imposing that (\[c4\]) be equal to (\[elbe\]), we obtain the set of equations $$\begin{aligned}
& &\frac{{\rho}^2}{f^2}CD=AB+CD{\omega}^2-AD\omega-BC\omega ,\nonumber\\
& &D^2+\frac{f^2}{{\rho}^2}\left[2BD\omega-{\omega}^2D^2-B^2\right]=1,
\nonumber\\
& &-C^2\frac{{\rho}^2}{f^2}+
A^2+{\omega}^2C^2-2AC\omega=1 .\label{c6}\end{aligned}$$ System (\[c6\]) has three independent equations for four variables and thus admits solutions. However, transformations (\[c5\]) are purely local, i.e. non integrable. This means that there exists a rotating reference frame such that the metric appears to be static, and the coordinates $t^{\prime}, {\phi}^{\prime}$ are admissible only on a geodetic. Further, note that system (\[c6\]) depends on $\frac{f^2}{{\rho}^2}$: for the solution (\[elbe\]) this quantity is equal to $\frac{a^2}{{\mu}^2}$. Therefore, the angular velocity of an observer in the


the Ernst equation (see [@Al]). Moreover, in such coordinates the field equation in B-Z involving $\psi$ assume the most simple form. However, these formal analogies are not sufficient to etablish a direct link between the two methods. In fact, in spite of the similarity between the pole trajectory formula and expression (\[E\]), we have not at our disposal a simple way to relate a transformed solution in our method with the number of solitons that can be added in the B-Z method.\
However, is interesting to note that in both cases, starting with a spatially homogenous metric with a $G_3$ group of motion, the resulting solution has a $G_2$ group of motion [@Jan]. We conclude saying that our method is also applicable for spacetimes admitting two spacelike Killing vectors.months. The range of precipitation throughout the year can be up to 3000 to 6000 mm! The terrain is exceptionally muddy and unsuitable for hiking or trekking. The routes also become slippery, and people may be more prone to accidents during these times.


     ORDER = ('Tagging', 4)

        def _valid_vcard_handle(self, vc_handle):
            # If there is already a tag by this name, complain.
            return (vc_handle and
                   ('-' != vc_handle[0]) and
                   ('@' not in vc_handle) and
                   (not self.session.config.get_tag_id(vc_handle)))

        def       def _pre_delete_vcard(self, vcard):
            session, handle = self.session, vcard.nickname
            return (Filter(session, arg=['delete',
                                         'group:%s' % handle]).run() and
                    DeleteTag(session, arg=[handle]).run())

    return GroupVCardCommand

class Group(GroupVCard(VCard)):
    """View groups"""

class AddGroup(GroupVCard(AddVCard)):
    """Add groups"""

class GroupAddLines(GroupVCard(VCardAddLines)):
    """Add lines


Another All party meet to be convened on August 18

Kolkata: The All-party meeting convened by Chief Minister Mamata Banerjee at Nabanna on Saturday continued for nearly two and half hours. The government has claimed the all-party meet to be successful but on 18 August an all-party meet will again be held to discuss the flood situation.

After the meeting Opposition Leader Surya Kanta Mishra said, “All party meet on flood situation can’t be conducted like this. Only legislative level meet can bring a solution. We have demanded a state level All party meet.”

Mishra said that the suggestion was accepted by the Chief Minister. An all-party meet will again be conducted on August 18 but it is not clear which parties will be present in the meeting. Among those present:(
<ariabbas> ongolaBoy: moi je suis le match sur www.maxifoot-live.com
<ariabbas> Bonne nuit Ã  ubuntu-cm
<ariabbas> ****
#ubuntu-cm 2013-02-04
<ariabbas> .
<ariabbas> .
<ariabbas> .
<ariabbas> .
#ubuntu-cm 2013-02-05
<ariabbas> .
<simplice_ndere> hi all
<warrens> simplice_ndere: hi
<simplice_ndere> warrens: whtat's news?
<warrens> ...nothing new
<warrens> i... don't know
<warrens> are you at ndere?
<simplice_ndere> warrens: no
<warrens> cauz i read simplice_"ndere"
<simplice_ndere> warrens: i was at Ndere
<warrens> ah, ok
<ongolaBoy> bon.. c'est un ancien ndÃ©rÃ© qui est , de temps Ã  autre _belge_ :) (pourquoi ? devinette pour vous)
<IzaneFG> simplice_ndere: :p
<ongolaBoy> yep mais la devinette porte sur le fait de savoir pourquoi il est de temps en temps belge ;)
<IzaneFG> moi monsieur, moi monsieur! :p
<warrens> ongolaBoy: il a de l'humour noir? :-O
<IzaneFG> ongolaBoy: son IP est souvent belge :-)
<warrens> IzaneFG: tu ouvre encore une autre question la. pourquoi son IP est souvent belge?
<warrens> et puis, sa ressemble a


ce qu'il a dit.
<ongolaBoy> oui j'avais compris
 * ongolaBoy disparait
<ariabbas> .
#ubuntu-cm 2015-02-03
<ariabbas> .
 * indy21 constate que y'a beaucoup de dynamisme.
<indy21> Personne pour se joindre Ã  moi pour actualiser le wiki. :-D
<ariabbas> .
 * ongolaBoy va le faire tout Ã  l'heure
<indy21> ongolaBoy: je parlais de l'intÃ©gralitÃ© hein!
<ongolaBoy> pas de problÃ¨me mon gars ...
<ongolaBoy> :)
<ongolaBoy> comme je disais Ã  cÃ©dric Ã©lie tantÃ´t au niveau de l'AUF on avait du dÃ©panner nous mÃªme le support d'ovh quand il fallait utiliser notre nuage privÃ© https://wiki.auf.org/wikiteki/ZA/Montr%C3%A9al/OVH/NuageD%C3%A9di%C3%A9
<ongolaBoy> Ã§a ne marchait pas pour GNU/Linux et ils n'avaient pas la solution
<ongolaBoy> je ne trouve pas long ce qu'il a Ã©noncÃ© .. enfin peut Ãªtre parce que j'ai l'habitude du wiki (et de surcroit MoinMoin)
<indy21> ongolaBoy: vous vous comprenez entre vous. :D
<ongolaBoy> en tout cas sifor the parameter regime considered. The result is in contrast with the interaction between two rigid or two flexible objects in tandem arrangement, and it may provide a physical insight into the understanding of the aquatic animals swimming in the bow wake of ships or staying in the bow wake of stationary structures.


of the tea cup and Biscornus are French eight-sided pin cushions which I plan on putting on edge of saucer. Then, the tea cups will be placed out of harm's way in my Stitching Room!!

I do plan on Stitching Room and Assisted Living "Rooms" will be pictured and toured thoroughly later in this week!!

About Me

My name is Deborah. I am now married, but husband is in a nursing home. I do not have any children, but we had the love of our life furbaby, Miprezious, who is nine years old. However, as an update, our beloved, Miprezious, did pass on September 05, 2010. I am 61 years young and retired prematurely due to disability.
NEWEST UPDATE
My beloved husband, Max, did loose his battle of deperately clinging on to hisAdjunctive atropine versus metoclopramide: can we reduce ketamine-associated vomiting in young children? a prospective, randomized, open, controlled study.
Pediatric procedural sedation and analgesia (PPSA) with ketamine administration occurs commonly in the emergency department (ED). Although ketamine-associated vomiting (KAV) is a less serious complication of ketamine administration, it seems to be cumbersome and not uncommon. The authors evaluated the incidence of KAV and the prophylactic effect of adjunctive atropine and metoclopramide in children receiving ketamine sedation in the ED setting. This prospective, randomized, open, controlled study was conducted in children receiving ketamine sedation in the ED of a university-affiliated, tertiary hospital with 85,000 ED visits, including 32,000 pediatric patients from October 2010 to September 2011. The primary outcome was a measure of the incidence of KAV in the ED and


my deep fryer with this Air Fryer and I'm glad I did that. This eBook will introduce you to the newest "must haves" kitchen appliance as of today. A brief explanation on what Hot Air frying is and discover the benefits you can get from using this. A great guide on the types of food for hot air frying. Learn and understand the functions of every basic parts and accessories of this device. A step-by-step guide on the basic operation of this kitchen appliance are also provided in this eBook. The best part about this eBook are the simple but delicious recipes that are included in this eBook. The directions for each recipes are easy to follow which made it really handy for us new users. Highly recommended!!!

ThisCoronary heart disease (CHD) accounts for every 1 of 6 deaths in the United States (US), with acute incidences estimated at 1.1M annually [1]. Of these, up to 40% are classified as ST-elevation myocardial infarction (STEMI) in which a clot blocks a coronary artery, leading to a change in the ECG ST wave [1] [5]. Because the heart muscle rapidly dies, time to reperfusion is critical [6] [7] [8]. While current CHD annual indirect costs are high, they are projected to further increase from $87B in 2013 to $130B by 2030 [9]. Early percutaneous coronary intervention (PCI) remains the standard of care in the US. However, PCI has not shown to be of benefit to STEMI patients living in rural communities due to increased transportation times. Efforts to


pyrrhic victory over the long haul.

6.For
law students or new attorneys considering a career in administrative law, what
do you think would be a good way of familiarizing themselves with the field?

I am a big advocate of finding an
issue or a cause that moves you and then trying a variety of tools to determine
which ones fit your personality and life choices.I wouldn’t recommend that a student pursue a
career in administrative law or tax law or estate law right out of the
gate.Instead, find something that really
makes you pound the table, exposes you to a wide range of experiences and let
your career unfold over time.I have
always been skeptical of 22-year-old law students who know they want to spend
their entire career in one area of the law.A lucky few may find the rightfungi harvested, 3.25 kg of SMS is produced \[[@pone.0237259.ref004]\]. Thus, SMS is a class of nutritious organic waste that may be worth reusing.

The use of SMS has been studied from many different perspectives \[[@pone.0237259.ref005]\]. In terms of the scale of utilization, large amounts of SMS have been used as fertilizer, as SMS possesses all essential attributes of organic manure after recomposting by natural weathering or any other process \[[@pone.0237259.ref006], [@pone.0237259.ref007]\]. SMS has been used as a substrate for culturing the same or other edible fungi \[[@pone.0237259.ref008], [@pone.0237259.ref009]\] and has been used for producing bacteria \[[@pone.0237259.ref010]\]. Due to its loose porous structure and unique biological properties, SMS can also be used as a biosorbent \[[@pone.0237259.ref011], [@pone.0237259.ref012]\] in wastewater treatment \[[@pone.0237259.ref013]\] or for the microbial degradation of polycyclic aromatic hydrocarbons


mushroom industry has been experiencing rapid development since 1978. Industrial production technologies started to advance after the new millennium, with nearly 50 different species of edible fungi being commercialized in China \[[@pone.0237259.ref028]\]. Among them (in order of total yield), *Lentinus edodes*, *Pleurotus ostreatus* and *Auricularia auricula* are the top three edible mushrooms \[[@pone.0237259.ref028]\], and *Flammulina velutipes* and *Pleurotus eryngii* are the two most industrialized mushrooms \[[@pone.0237259.ref029]\]. Some edible fungi, such as *Pleurotus citrinopileatus*, are only locally produced, and Jilin Province is the second largest producing region of *P*. *citrinopileatus* \[[@pone.0237259.ref030]\]. Some new edible fungal species, such as *Auricularia cornea*, have also recently been developed and compete with *A*. *auricula* on the market \[[@pone.0237259.ref031]\]. We first investigated the feeding adaptability of TML to the SMSs of the five most  24 ± 5                   90 ± 5          60   3
  *Lentinus edodes*             78% saw dust, 16% wheat bran, 2% gypsum, 1.5% corn meal, 1.5% sugar, 0.5% urea and 0.5% calcium superphosphate   22 ± 5                   90 ± 5          60   3
  *Pleurotus citrinopileatus*   80% corncob powder, 18% wheat bran, 1% lime powder and 1%


for its suitability for the growth and development of young TML compared to conventional feed comprising wheat or rice bran. The testing methods were similar for each SMS group. For each test replicate, 50.0 ± 0.1 g of feed was weighed using an electronic balance (FB224, Shanghai Shunyu Hengping Scientific Instrument Co., Ltd., China. Max = 220g, Min = 0.01g, e = 1mg, d = 0.1mg) and then placed in a plastic box (140 × 105 × 50 mm) covered by a 50 × 50 mm mesh air vent lid. All boxes were placed in the insectary for 24 hours prior to the introduction of TML. This process was necessary to keep the powder temperature and humidity the same as that in the environment to avoid unnecessary stimulation9^th^ instars as middle-aged larvae, and larvae of this age were used for the tests. As described above, each test replicate consisted of 50.0 ± 0.1 g feed placed in a plastic box (140 × 105 × 50 mm). After 24 hours, 30 larvae (mean mass 19.4 ± 1.3 mg) were introduced into each box and reared for 45 days.

To consider the possible effect of green feed on insect growth and development, each treatment was further divided into two groups: group A was fed dry feed only, whereas group B was fed dry feed and 2.0 ± 0.1 g of fresh watermelon peels. The watermelon peels were provided every three days until the end of the 45-day rearing period (i.e., a total of 15 times). All larvae were


 ---[^3^](#t002fn003){ref-type="table-fn"}
  *Pleurotus ostreatus* SMS         0.0 ± 0.0 c                                          ---[^3^](#t002fn003){ref-type="table-fn"}
  *Auricularia cornea* SMS          0.0 ± 0.0 c                                          ---[^3^](#t002fn003){ref-type="table-fn"}

^1^Values (mean ± SE) followed by different letters within thenortheastern China, rice bran is not only cheaper but also easier to obtain than wheat barn and requires lower transportation costs than wheat bran. Therefore, rice bran could emerge as a valuable feed for TML. The only disadvantage is that the rice bran is not tolerant to long-term storage and, if deteriorated, produces aldehydes and ketones that can damage the livers of animals \[[@pone.0237259.ref043]\]. For this reason, a large amount (80%) of rice bran is not used effectively or is discarded as waste in China \[[@pone.0237259.ref044]\].

The use of SMS could greatly reduce the costs of rearing TML, as the material is cheaper than conventional feed and is easy to obtain from the mushroom industry. Although TML are mainly used as poultry or fish feed in most countries, they


subjects performed the ARM and WB lottery tasks in a randomized order at each elevation, counterbalanced across the two tasks. They completed both choice tasks at low elevation before performing them at high elevation (Figure [1C](#F1){ref-type="fig"}). It has been previously shown that increasing elevation results in more pronounced changes to postural control variables than decreasing elevation (Adkin et al., [@B2]). In always presenting the Low elevation condition first, we intended to capitalize on these order effects to maximize potential changes in risk-sensitivity due to threat. Ideally, we would test and re-test as many conditions as possible to examine consistency of choices over the different conditions. However, due to the overall length of the experiment and to minimize mental fatigue, we had subjects repeat one of these tasks, whichwould not straightforwardly predict the changes in risky choice behavior.

Increased risk-aversion in movement control could arise from a reduced valuation of the reward associated with completing a movement. This would manifest as a lower α value and a lower fR. Alternatively, risk-aversion could arise from an overweighting of the chance of a movement error that would lead to a fall. This would manifest as a reduced γ value, reflecting an overweighting of small-probability events.

Skin conductance
----------------

We measured changes in skin conductance throughout this experiment using the BIOPAC MP35 acquisition hardware, collecting data at 1000 Hz. Disposable electrodes were placed on the subject\'s left hand, on the distal phalanx of the index and middle fingers. Skin conductance level (SCL) for each subject was calculated as a percent increase over a


less than (as in WB) the mean fR at Low elevation. So although Figure [5A](#F5){ref-type="fig"} suggests that mean fR increases slightly, or stays the same, with elevation, removing an outlying subject visually establishes that mean fR actually decreases slightly, or stays the same, with elevation. We repeated all analyses with this outlier subject removed; however, there is no resulting change in our statistical outcomes, nor do our overall findings differ with the exception of the aforementioned trends of mean fR compared between Low and High elevations. Thus, we included the outlying subject for the remainder of the analysis presented here.

A comparison of individual fR values between the two motor tasks are shown in Figure [5B](#F5){ref-type="fig"} for both Low and High elevations. Here, a data point on the lineappear to align with the findings of Neyedli and Welsh ([@B22a]), who noted a preference for probability information over value information when in a motor decision-making task. Participants were offered choices between target configurations for a pointing movement, in which a penalty region overlapped the target. By varying the amount of the point-based penalty (value information) as well as the distance between the target and penalty (probability information), the authors found that participants were more sensitive to probability, preferring configurations with a larger chance of hitting the target to configurations with lower penalty values. If individuals process probability more readily than value during movement selection, it holds that an altered decision strategy would manifest itself more clearly in probability weighting than in utility.

Interestingly, subjects only altered probability weighting


---
abstract: 'I introduce a method to obtain the stress-energy tensor of the perfect fluid by adding a suitable term to the Einstein-Hilbert action. Variation should be understood with respect to the metric.'
author:
- 'E. Minguzzi [^1]'
title: 'Inclusion of a perfect fluid term into the Einstein-Hilbert action'
---

Introduction
============

Since the gravitational side of the Einstein equations is variational one would like to obtain the right-hand said from the variation of a matter Lagrangian. This raises the problem as to whether the dynamical equations for the most straightforward form of matter, the perfect fluid, are variational in character. These equations, namely the continuity and the Euler equations have been given variational formulation following different approaches [@taub54; @hawking73; @schutz77; @baylin80; @brown93; @poplawski09; @minazzoli12].

The most common strategy [@taub54; @carter73; @kijowski79; @kunzle84; @carter89; @kijowski90; @carter94; @comer93;\beta} u^\alpha u^\beta=-1$, and for barotropic fluids the constitutive relation, $\rho=f(p)$, establishes a functional contraint between the density $\rho$ and the pressure $p$.

Barotropic fluids
-----------------

Let us show that there is indeed a Lagrangian $L_{(m)}(g_{\alpha \beta} )$ which returns the perfect fluid stress-energy tensor with a chosen-in-advance functional dependence between $\rho$ and $p$. We claim that the action with this property is $$\begin{aligned}
 \label{act}
S[g_{\alpha \beta}]&=\int \left (\frac{1}{16\pi }(R-2 \Lambda) +  P\left(\sqrt{-g^{\alpha \beta} v_\alpha v_\beta}\,\right) \right) {{\rm d}}x ,\end{aligned}$$ where function $P(x)$ is determined by the differential equation $$\label{msc}
xP'-P=f(P).$$ Observe that the left-had side is $P^*(P'(x))$ if the Legendre transform $P^*$ exists. Equation (\[msc\]) can be easily integrated since the variables can be separated $$x=\exp\left(\int^P \!\!\!\frac{{{\rm d}}\tilde P}{f(\tilde P)+\tilde P}\right).$$ At the stationary point the integral curves of $v^\alpha:=g^{\alpha \beta}


v_\alpha v_\beta} }, \\
\rho&:= x P'(x) -P(x),\\
p&:=P(x),\end{aligned}$$ where $u^\alpha$ is interpreted as the covariant velocity of the continua and $\rho$ and $p$ as density and pressure, respectively. With these definitions $T_{\alpha \beta}$ takes the form (\[jus\]) and by Eq. (\[msc\]), $\rho=f(p)$ as desired.

It is natural to ask if a similar result could be obtained given as data a vector field $v^\alpha$. Indeed, it can be done using as action $$\begin{aligned}
 \label{ace}
S[g_{\alpha \beta}]&=\int \left (\frac{1}{16\pi }(R-2 \Lambda) +  P\left(\frac{1}{\sqrt{-g_{\alpha \beta} v^\alpha v^\beta}}\right) \right) {{\rm d}}x ,\end{aligned}$$ where $P(x)$ is related to $f$ as before, at the stationary point $x$ is still the index of the fluid, and $p$ and $\rho$ depend on $x$ as before. However, it is not true that $v^\alpha$ is the dynamical velocity of the fluid,per mulberry take on sacks most economical Mulberry laptop bag, a good option to discover you will certainly be on the web. You can find world wide web mulberry alexa travelling bag anywhere which often design and sell custom made shopping bags to get a part of their unique groundbreaking price level. An excellent using the net designer purse reserves should be Eurohandbag, whereby you would hunt for famous label mulberry such as shoes names for price reduction expenses. Can't consideration the occasional mulberry carrying cases low cost those same designer purses, the quantity belongings should still just be relied on. That this Mulberry designer bags on this site is the best good appliance regarding. Another Mulberry designer handbag is probably acknowledged due to its old fashioned options.


       baos.close();
          }catch(IOException e){System.out.println(e.getMessage());}
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          return imageBytes;

Thanks!

A:

A png consists of a lot of image meta data as well as the raw image data. That is what is giving you crazy 680 bytes.

The effect of target orientation on the visual acuity and the spatial frequency response of the locust eye.
The eye of the locust was shown to distinguish between vertical and horizontal alignments of narrow slit targets, and responded more strongly with horizontal alignments. In all cases the response increased as slit width decreased, to a limit of about 0.1 degrees. Similarly the eye responded more strongly to gratings in horizontal alignment, with a peak in response close to a spatial frequency of 3 cyc/deg. These results are discussed and comparisons are made with previous findings on insect and other eyes.


external data
d3.csv("treedata.csv", function(error, data) {

// *********** Convert flat data into a nice tree ***************
// create a name: node map
var dataMap = data.reduce(function(map, node) {
 map[node.name] = node;
 return map;
}, {});

// create the tree array
var treeData = [];
data.forEach(function(node) {
 // add to parent
 var parent = dataMap[node.parent];
 if (parent) {
  // create child array if it doesn't exist
  (parent.children || (parent.children = []))
   // add node to child array
   .push(node);
 } else {
  // parent is null or missing
  treeData.push(node);
 }
});

  root = treeData[0];
  update(root);
});

function update(source) {

  // Compute the new tree layout.
  var nodes = tree.nodes(root).reverse(),
   links = tree.links(nodes);

  // Normalize for fixed-depth.
  nodes.forEach(function(d) { d.y = d.depth * 180; });

 power, gas or other
> commodity purchase and sale documentation to confirm whether they possess
> this right.  The failure to exercise this right may constitute a waiver
> and foreclose access to critical performance and payment assurance,
> threatening to alter the risk assessment and mitigation considerations
> upon which the transactions were originally based.  Furthermore, a failure
> to declare a credit event or default against Enron could cause a default
> on the part of the counterparty itself in collateral or ancillary
> arrangements, such as its financing.  The potential for a domino effect,
> causing defaults or credit events in a series of transactions, is
> considerable.
>
> 	It is uncertain whether Enron's efforts to obtain new lines of
> credit will be sufficient to overcome the current difficulties.
> Regardless, it is


By Easton standards, Dunsby is a newcomer, having lived in town for a decade or so. He will square off against Easton Coalition candidate Val Buckley, a Republican, who has been director of the Easton Senior Center for more than 20 years.

The Republican Town Committee's vacancy subcommittee held a two-hour emergency meeting Friday night to figure out who its candidate ought to be.

"There was a lot of tension because of the short time before the election. Really, the campaign should have started by now," said one member of the panel who spoke on condition of anonymity. "There was campaign material that was already on its way to the printer that had to be canceled."

Before choosing Dunsby, some on the vacancy subcommittee wanted to interview prospective candidates or reconsiderof Engineering and Technology, a Professional Member of the Association for Computing Machinery, a Member of the Institute of Advanced Motorists and an Associate Member of the Highland Reserve Forces' and Cadets' Association.

24 February 2015

Stewart Stevenson, SNP MSP for Banffshire & Buchan Coast, has welcomed new statistics showing an increase in the number of Home Fire Safety Visits in Grampian – and has paid tribute to the hard work and dedication of Scotland’s fire service.

Figures show that the number of Home Fire Safety Visits in Scotland rose from 56,251 in 2012-13 to 70,686 in 2013/14 – including a rise from 947 to 4369 in Grampian.

Mr Stevenson said:

“The men and women working in Scotland’s fire service do excellent work in our communities to promote safety and prevent fires –


and Cullen – as well as being available for hire. The new dredger, which was designed by Macduff Ship Design Ltd, will be capable to dredging to a depth of 8m with its bow-mounted excavator.

Commenting, Stewart Stevenson said:

“This is great news for Buckie and for Macduff Shipyards. It serves to further develop the already vast expertise of the company and, importantly, keeps these skills in the local workforce.

“The dredger is an important asset in the maintenance of our local harbours and I look forward to seeing it in service next spring.”

Lintmill-based National fuel and lubricant supplier Northern Oils announced this week that they will commence operations from their new site at Workington on 1 March. The Cumbrian Storage Terminal represents a key location for the group who havethe Tigers and the Tide there was huge recruiting activity right here in Baton Rouge. Yes, many of our top athletes were being wooed by Alabama and Coach Orgeron was fighting hard to keep them. We are not qualified to update you on that. However we can tell you that a room at the Renaissance Hotel in Baton Rouge was filled that same week with top academic Louisiana high school juniors and seniors. Any parent or grandparent of an academically excellent Louisiana High School junior or senior knows and is possibly dismayed that a Saban like recruiting push is being made by Alabama to bring these young academic talents to Alabama. They know the value they can add to Alabama's medical, financial, tech, and aerospace economic development activity.


Large-scale monocyte enrichment coupled with a closed culture system for the generation of human dendritic cells.
Conventional methods for generating monocyte-derived dendritic cells (DC) for clinical trials utilize the property of plastic adherence to select monocytes from leukapheresis samples. This method is labor-intensive and has the potential for contamination at various steps. We evaluated a large-scale monocyte enrichment procedure using a cell selector (Isolex 300i(R)) followed by culture in a sterile bag system (Stericell(R)) for generation of DC. DC generated in tissue culture flasks after monocyte selection by plastic adherence were compared to those generated in Stericell(R) bags after monocyte enrichment by negative selection with the Isolex(R) 300i. DC were matured with lipopolysaccharide and pulsed with a peptide derived from the melanoma antigen gp100. Peptide-pulsed DC cultured by thetwo techniques were evaluated for phenotype, viability, ability to induce allogeneic and peptide-specific autologous proliferative responses as well as peptide-specific cytotoxic T-cell responses. The mean monocyte yield from leukapheresis collections was 17+/-2.4%, which increased to 52+/-11% after Isolex(R) selection. The DC yield of plated mononuclear cells from flasks or bags was 2.7+/-0.96% and 4.84+/-2.65%, respectively. DC cultured by both methods expressed high levels of CD86, CD80, CD40, CD83, CD44, CD11c and CD58, and was comparable in their ability to induce allogeneic and peptide-specific autologous proliferative responses as well as gp100 peptide-specific cytotoxic T-cell responses. These results indicate that potent monocyte-derived DC can be generated in a closed culture bag system after monocyte enrichment by immunomagnetic negative selection. Due to the closed nature of the enrichment and culture systems,


to the electrically conductive leads, and encapsulating the IC chip, the support and the electrical connections in, for example, a plastic package.
Recently, very high-density magnetic memories, such as magnetic random access memories (MRAMs), have been proposed to be integrated together with CMOS circuits. Magnetic random access memories employ one or more ferromagnetic films as storage elements. A typical multilayer-film MRAM includes a plurality of bit or digit lines intersected by a plurality of word lines. At each intersection, a ferromagnetic film is interposed between the corresponding bit line and word line to form a memory cell.
When in use, an MRAM cell stores information as digital bits, the logic value of which depends on the states of magnetization of the thin magnetic multilayer films forming each memory cell. Asin a team, and started to build friendships."

"Gardening allowed them to make something with their hands, and gave them a sense of accomplishment. We have seen an incredible change in them."

Samar, 48, fled to Jordan with her husband and five children after two of her brothers-in-law were killed.

Zaatari camp has been home to her family for over two years. Prior to the war in Syria, Samar was a headmistress at a secondary school in Daraa and three of her daughters were attending university and studying engineering, architecture and physics.

They had a large house with a beautiful garden. Summers were spent cooking feasts with homegrown vegetables and sipping coffee under the shade of an olive tree.

"When we garden, we feel happy because there's something to do, such as watering


FDA needs to follow existing FDA protocols and stop being driven by the prevailing winds from political forces. FDA is the final authority on regulation of drugs and should not be second guessed people who do not understand medicine and science, much less attempt to superimpose layer upon layer of regulations on top of existing FDA prescribing guidelines tailored to each pain medication.

CDC is trying to drive policy based on their poorly written and poorly understood 2016 Opioid prescribing guidelines, proving themselves incompetent and not grounded in the best interests of the US public, especially not in best interests of chronic pain patients who depend on analgesics for quality of life. CDC has castigated the US chronic pain community and destroyed the lives of millions of honest, law-abidingThrombolytic therapy is an only determinant factor for stroke evolution in large anterior choroidal artery infarcts.
Large anterior choroidal artery (AChA) infarcts are frequently associated with stroke evolution. This study aimed to investigate the major determinants for stroke evolution in patients with large AChA infarcts. We studied 118 consecutive adult patients with acute large AChA infarcts. The diagnosis was confirmed as abnormal hyperintensities in 3 or more rostracaudal magnetic resonance imaging slices (5 mm thickness) using diffusion-weighted imaging within typical AChA vascular regions. Stroke evolution was defined as neurologic deterioration with an increase in National Institutes of Health Stroke Scale (NIHSS) score by at least 4 or an increase of NIHSS score in motor function by at least 2 in 7 days after stroke onset. Forty-seven (39.8%) patients developed


stroke evolution. Thrombolytic therapy was inversely associated with the occurrence of stroke evolution (P = .004). Using multivariate analysis, thrombolytic therapy was the only protective determinant for stroke evolution (adjusted odds ratio, .08; 95% confidence interval, .01 to .67). Patients with large AChA infarcts receiving thrombolytic therapy had less unfavorable long-term functional outcome than those not receiving thrombolytic therapy (adjusted odds ratio, .11; 95% confidence interval, .02-.75). Thrombolytic therapy is an only determinant factor for stroke evolution in large AChA infarcts, which reduced the risk of stroke evolution and improved functional outcome.band)" released "Blood for the Blood God" as a free flexi-disc with the issue #95 of White Dwarf, Games Workshop's in-house publication.

In the late 1980s the death metal band "Bolt Thrower" wrote lyrics dedicated to the Warhammer 40,000 universe and used 40k artwork on the cover of their second album, Realm of Chaos.

In the early 1990s Games Workshop created its own short-lived record company, Warhammer Records. The only band under this label was D-Rok (who published one album, Oblivion, in 1991). A fragment of D-Rok's song "Get Out of My Way" was used in the computer game "Space Hulk", published by Electronic Arts in 1992.

In the early 2000s the German label Art of Perception produced a 12 part soundtrack vinyl series followed by three CD compilations. The task


therapeutic penetrating keratoplasty, amniotic membrane transplantation and epithelial transplantation techniques, it should be taken into consideration in selected cases. These alternative treatments constitute considerable technological progress, but have some impediments, especially concerning their limited availability in developing countries, more specifically the deficit of corneal donor tissue for corneal transplantation ([@b3-etm-0-0-8964]).

2. Purpose and therapeutic effects
==================================

The main purpose of this surgery is to recover the integrity of the corneal surface to prevent gradual corneal ulceration and secondary infection, as well as to achieve several other secondary effects: relieving pain, reducing topical medications, enhancing the aesthetic appearance and providing an option to invasive surgery or enucleation ([@b3-etm-0-0-8964]).

The therapeutic effects of CF surgery are probably promoted by several mechanisms:

i\) The conjunctival flap covers the affected corneal tissue and prevents tears, proteolytic enzymes,fitting of a cosmetic contact lens or scleral shell. Some advocate that a proper examination of opaque blind eyes is difficult and that prophylactic enucleation should be performed because they may harbor malignancy ([@b16-etm-0-0-8964]).

The list of indications has expanded over time to include chronic infectious keratitis and tectonic indications such as peripheral marginal ulcerations and corneal melts ([@b7-etm-0-0-8964],[@b16-etm-0-0-8964],[@b19-etm-0-0-8964]). Corneal ulcers refractory to medical treatment can rapidly progress to a corneal perforation that can lead to debilitating visual consequences, so rehabilitation of the integrity of the ocular surface is mandatory to conserve the visual function ([@b6-etm-0-0-8964]).

CFs are usually not successful in cases of active refractory bacterial and fungal corneal ulcers. However, in emergency situations, especially in countries that lack cornea donors, CF surgery can be employed as a temporary


disturbance of the local architecture, possibly leading to limbal stem cell deficiency ([@b13-etm-0-0-8964]) and affecting the conjunctiva that may be necessary for glaucoma filtering surgery ([@b8-etm-0-0-8964],[@b14-etm-0-0-8964]).

ii\) Bipedicle bridge flap (bucket-handle) is used for small central or paracentral corneal lesions that do not need coverage of the entire cornea and involves a 180-degree peritomy, separation of the conjunctiva from the underlying Tenon\'s capsule, an incision parallel to the limbus and mobilization of the conjunctiva over the corneal ulcer ([@b12-etm-0-0-8964]). Advantages: assessing the remaining uncovered cornea and anterior chamber.

iii\) Single pedicle flap (racquet flap) is a smaller racquet-shaped perilimbal CF that is mobilized to cover perilimbal corneal lesions. Advantages: less prone to retraction.

iv\) Advancement flap is used for paralimbal lesions, usually in conjunction with a lamellar corneal patch or scleral([@b29-etm-0-0-8964]) in a case series of two patients diagnosed with Acanthamoeba keratitis. The goal of deep lamellar keratectomy combined with CF is to remove the infected tissue in order to decrease the number of microorganisms and to eliminate necrotic tissue. Also, the CF brings conjunctival vessels and lymphatic channels that increase the immune response against the amoeba.

Adding cryotherapy to flap surgery may have a cumulative effect. Cryotherapy of the corneal surface at temperatures of -50 to -60˚C not only denatures the pathogen\'s cell walls but can also remove part of the antigen-antibody complexes and decrease inflammation caused by the area of ulceration and neovascularization ([@b8-etm-0-0-8964],[@b25-etm-0-0-8964]).

In an attempt to increase the efficiency of CF surgery, fibrin glue has been used as an adhesive to maintain the flap in place.


or too loose. Sutures that are too tight may cut through a tight CF and a loose CF may not heal and detach in the postoperative period ([@b29-etm-0-0-8964],[@b31-etm-0-0-8964]).

Complications after CF surgery are relatively uncommon. The most frequently reported complications include flap retraction, conjunctival buttonholes and erosions, epithelial inclusion cysts, corneal perforations ([@b3-etm-0-0-8964]), and occasionally ptosis ([@b2-etm-0-0-8964]). However, positioning of a CF over the cornea has some disadvantages: Hindering the visualization of intraocular structures, compromising the penetration of topical medications (e.g., glaucoma medications) and a poor cosmetic result in case of thick flaps. Also, as mentioned before, removal of limbal epithelium harboring stem cells can possibly affect the stability of the ocular surface and this should be considered in cases in which flap removal and subsequent keratoplasty are planned.

Anothertraining, but cheek strength remained unchanged. Training effects were not related to training condition (sequential vs. concurrent), nor were specificity effects observed for direction of exercise. Significant decreases in lingual strength were noted 2-4 weeks after exercise was discontinued. The findings replicate those of earlier studies demonstrating that lingual strength may be increased with a variety of exercise protocols and confirm that detraining effects may be observed when training is discontinued. The findings further suggest that the lingual musculature may demonstrate less dramatic training specificity than what has been reported for skeletal muscles.


one of the worst starting QBs in the NFL. Eric Berry (the next Ed Reed) has underwhelmed. Rolando McClain might be out of football next year. And so on and so on...

The quality of a given draft is always relative and can't really be assessed a lot of the time until several years down the road. You can always say "so and so isn't worthy of a top 10 pick," but if a given player is one of the 10 best talents in the class then why the hell not?

With the rookie wage scale most of the downside of missing on a pick is now pretty much gone. Just pick the best player available that makes sense for your team, upgrade your overall talent level whenever possible, andare posers. If they answer “my child’s First Communion,” they check out.

Use “jawn” in a sentence. This is an easy one. Jawn can be used to described just about anything. You could even say “Bring that jawn to the jawn or I’m outta this jawn” and that’d be an acceptable answer.

Have you heard anyone mention Wawa? Then there are probably zero Eagles fans in the vicinity. If they say they prefer “GetGo,” they’ve been bussed in from Western Pennsylvania.


toured the area of the airport.) Five of the 30 kilometers approved by the Cabinet have been built since September.

"The advantage of building here and along the Egyptian border is that there are no structures adjacent to the fence, not one kilometer away and not two kilometers away, that could serve as an opening," said the prime minister. "That's not the case in Gaza. This is potentially not the case in Judea and Samaria, where there are structures built adjacent to the separation line you are considering forming, either. When you build a fence there, you have to take into account that tunnels are being built underneath. Those who said that territory doesn't carry importance in the modern era should go to Gaza.

Netanyahu takes air tour of areahigh standards when it comes to personalized experiences. Customers in the B2B space don’t want to be delivered messaging tailored to B2C consumers, and vice versa. AI enables marketers to create audience personas and understand specific purchasing motivations. With these tools, marketers can target their target demographics and speak directly to the buyer’s needs and pain points.

Marketers can now focus on the big picture.

With the help of AI, marketers can now look at the big picture and implement this holistic view to refine their strategies. AI isn’t going to replace marketing teams, but it will take over formerly automated tasks so that they can focus on big-picture decisions. In particular, all-in-one media monitoring platforms cut down time of sifting through millions of data sources, so marketers can spend