$S$ symmetry, the first six variables fully specify the relative positions of the three particles and the wavefunction dependence simplifies to $\Psi(r_{12}^+, r_{12}, r_3, \cos\alpha,\cos\beta,\gamma)$. Consider now two symmetry operations which change the sign of the wavefunction and keep the distances unchanged: parity $P_I$ and exchange $P_{12}$ between particles 1 and 2. The exchange flips the sign of all three $\cos\alpha,\cos\beta,\gamma$ while the parity changes only the sign of $\cos\alpha$. The action of $P_I P_{12}$ on $\Psi$ leads to $$\Psi(...,\cos\alpha,-\cos\beta,-\gamma)=
\Psi(...,\cos\alpha,\cos\beta,\gamma)$$ showing that the wavefunction is even in the simultaneous sign flip $(\cos\beta,\gamma) \to (-\cos\beta,-\gamma)$. Applying the exchange operator $P_{12}$ to the wavefunction and taking advantage of the previous property gives us $$\Psi(...,-\cos\alpha,\cos\beta,\gamma)=
-\Psi(...,\cos\alpha,\cos\beta,\gamma)$$ suggesting that there is a node determined by the condition $\cos\alpha=0$. It is also clear thatcomparison with other two- and three-electron CI calculations we estimate that the order of magnitude of the basis set CI bias is $\approx$ 0.01 mH (miliHartree) for two electrons and $\approx$ 0.1 mH and for three electrons (despite the large number of virtuals the CI expansion converges relatively slowly[@kutz] in the maximum angular momentum of the basis functions, in our case $l_{max}=4$). The pseudopotentials we used were identical in both QMC and CI calculations.

The first two rows of Tab.\[tab\_xx\] show the total energies of variational and fixed-node DMC calculations with the trial wavefunctions with HF nodes together with results from the CI calculations. For $^3P(p^2)$ the energies agree within a few hundredths of mH with the CI energy being slightly higher but within two standard deviations from the


Reynolds, [*Unexpected symmetry in the nodal structure of the He atom*]{}, submitted. Another system with such symmetries is the $\mathrm{H}_2$ $^3\Sigma_u^+(1s\sigma2p\sigma)$ state, first analyzed by H. M. James, A. S. Coolidge, and R D. Present, J. Chem. Phys. [**4**]{}, 184 (1936). K. Rektorys, [*Survey of Applicable Mathematics*]{}, Kluwer, 1994. D. Pedoe, [*Geometry Comprehensive Course*]{}, Dover, New York, 1988. J.G. Semple, G.T. Kneebone, [*Algebraic Projective Geometry*]{}, Oxford University Press, Oxford, 1979. P. Griffiths and J. Harris, Principles of Algebraic Geometry, Wiley, New York, 1978. W. Kutzelnigg, in [*Explicitly Correlated Wave Functions in Chemistry and Physics*]{}, Ed. J. Rychlewski, Kluwer, Dordrecht 2003, pp.4-82 and references therein. I. Ovcharenko, A. Aspuru-Guzik, W. A. Lester Jr., J. Chem. Phys. [**114**]{}, 7790 (2001). A. K. Wilson, T. v. Mourik and T. H. Dunning,is a contractual (or statutory) obligation for attorney's fees." 627 P.2d at 66. Although we do not adopt a rigid rule that attorney *123 fees may never be awarded for defending a counterclaim, we do caution that it should be the exception and not the rule to do so.
This cause is reversed and remanded for a trial by jury on plaintiff's complaint, and for reconsideration of the amount of any attorney fees to be allowed.
IT IS SO ORDERED.
SCARBOROUGH, C.J., and RANSOM, J., concur.


as a protected harbor for the numerous merchant ships and frigates usually anchored there, six miles upriver from the Atlantic coast. But this serenity was merely the calm before the storm, although not a storm with which the people of Lisbon were familiar. If an actual storm were coming, its rain and winds would eventually provide a warning, however late in coming. Even if unpredicted, a storm or hurricane would at some point no longer remain hidden over the horizon, and there would be time, however short, to try to prepare for the arrival of the flooding storm surge and the destructive wind waves—a last-minute chance to take shelter or move inland to higher ground. But what if the impending disaster from the sea was not brought byin a harbor usually caused by a changing wind, similar to water sloshing up and down at opposite ends of a bathtub. While not an ideal choice, "tsunami" has now become the accepted term. However, "tidal wave" is still used frequently in the popular media.

As we have already seen with the Lisbon earthquake, a tsunami can travel thousands of miles. This is because it is a very long water wave, and such long waves do not lose energy very quickly. A long wave also travels very fast; the deeper the water depth, the faster it travels. Over the deep ocean (two to three miles deep) a tsunami can travel 400 miles per hour, about the speed of a jet airliner. The other important characteristic of a tsunami is


can use seismic data to quickly determine whether an earthquake is likely to have generated a tsunami (namely, been tsunamigenic). We have mentioned four types of seismic waves (P, S, Rayleigh, and Love waves), but there are others (T, W, etc.), and these are being analyzed in different ways to see which ones might indicate a high chance that a tsunami was generated. The second method uses GPS data. The primary use of GPS data is to determine the accurate position of a GPS receiver. Using installed high-precision GPS stations, one can measure how the land moved, vertically and horizontally, immediately after an earthquake and even how the seafloor moved after a submarine earthquake. Not only does this allow one to determine whether there was enough vertical movementlead to loss of life and cause serious economic impacts. The two most important examples are El Niño, which occurs every two to seven years, and long-term climate change, especially with respect to the effects of global warming where the most serious effects could possibly show up in only a few decades. Both are global phenomena with worldwide impacts. Both demonstrate the immense power of the sea on a planetary scale and its critical role in driving weather patterns around the world and in modifying our climate. Being able to predict the onset and effects of El Niño and being able to predict the effects of global warming can be of great economic benefit and can save lives by allowing us to prepare for future dangerous conditions. In


Studies_ , vol. 18, no. 2, pp. 150–153.

.Holland, F.W., 1868. "On the Peninsula of Sinai," in _Proceedings of the Royal Geographical Society of London_ , vol. 12, no. 3, pp. 190–195; see especially the comments at the end by Sir Samuel Baker. Many other authors have also written about Moses' knowledge derived by living in the wilderness, some of them referring to the work of Artapanus (80–40 BC) referenced in note 15.

.Several other points are relevant: (1) No other ocean phenomenon could have been predicted for this purpose except the tide. Some authors have hypothesized a tsunami washing over the Egyptians that was due to an earthquake or volcanic eruption, but that would have been totally unpredictable, and was extremely unlikely to have occurred just at the rightto spring and back). His writings have been lost, but he was quoted by Strabo in _Geography_ (AD 22), the earliest surviving Greek or Roman written work that provided insights about the tide. Strabo, _The Geography of Strabo_ , transl. Horace Leonard Jones, 8 vols., Harvard Univ. Press, Cambridge, MA, 1969; originally written by Strabo sometime before AD 24.

.Strabo, op. cit. The earliest advances in astronomy, including development of the zodiac reference system for the night sky, took place in Babylonia. This difference in height between two successive high waters is now called a _diurnal inequality_ (and we will see later in this chapter why this occurs).

.Harris, op. cit.

.The two primary sources for the story of Alexander the Great and the tidal bore are: Quintus Curtius Rufus, ca.


white gown while marrying her longtime girlfriend Kim Catullo on Saturday in one of the most high-profile same-sex weddings of an elected official, the Times reports: http://nyti.ms/KT6b1X

* The Post reveals some defendants and other details of sexual and child abuse cases involving ultra Orthodox Jews, while Brooklyn District Attorney Charles Hynes continues to keep the names secret: http://nyp.st/KE0T7I

* Former gubernatorial candidate Carl Paladino is supporting primary foes of the four state Senate Republicans who voted for same-sex marriage, saying he is going after those who broke promises, the Buffalo News reports: http://bit.ly/KFis7zencoded binary stream of bits, expanding every eight bits of input data into fourteen, with an additional three bits to separate words, as is well known in the art. In the CD-R format, 24 of the resultant 17 bit symbols are assembled into a frame. 98 frames are grouped together to form a sector. A layered level of error correction is accomplished on a "diamond" or combined lateral and slant pattern across the frames to avoid the total loss of a frame of data, as is known in the art. Thus, each sector comprises a totality of 98 frames of data, together with a sector header, synch information and layered ECC distributed across the frames and sectors. The CD-R media does not provide molded sector or block identification


arise from crashing waves and rising water levels, but also from velocity of driven water. Wind speeds increase towards the water surface, and are capable of lifting water in sheets from the natural body of water. These sheets of water are greatly accelerated and propelled towards the shore, and may impinge on the susceptible ground structure with great force, thereby causing damage by erosion.
When this water strikes ground surfaces, significant erosion may ensue. Examples of ground surfaces and structures which are susceptible to such attack include natural formations such as bluffs and dunes, but also artificial structures such as shoulders of roads, berms, drainage ditches, and the like. Such structures are typically formed from loose fill dirt, sand, light gravel, and other materials lacking resistance to erosion presentwith the BlackBerry maker deciding Apple was lying -- the iPhone wasn't possible.

RIM's opinion was that a phone with all of the 2007 iPhone's functionality and large touchscreen could not be used without being near a power supply at all times. Shacknews poster, Kentor, heard from his former colleagues:


Q:

What is the difference between "retired out" and "retired not out" in cricket?

I have seen the terms on scoreboard like: "retired out" or "retired not out". What does it mean and what is the different between there?
Reference:

Mohammad Hafeez was retired out in ICC Champions Trophy Warm-up
Matches, 2013 vs South Africa
Gordon Greenidge was retired not out in 5th Test vs India.

Also I have seen "retired out" only on practice matches. Is there any case of "retired out" in international matches?

A:

Retired not out
If a batsman retires during his innings because of injury, illness or any other unavoidable reasons, and doesn't resume his innings, he is considered as retired not out. He can resume his innings after the wicket is fallen or other batsmen is retired. Retired not out is consideredof our spaceship Earth, so it stays clean for all of you,” Oey said.

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Subscribe to the Roswell Daily Record today.

Using his laptop, Oey showed a PowerPoint of things he has seen while biking across the country, such garbage, a sea turtle with a straw in its nose, a deceased bird full of plastic and California’s superbloom. He also showed a photo of 150,000 children in Montreal protesting climate change a couple weeks ago.

Through the slower pace of cycling, Oey said it is easier to see how trash circulates from roads to rivers, and eventually how plastic and carbon dioxide pollution affects the ocean. Oey also teaches biking safety and he told a story about how he and some other bikers rescued a salamander by blocking it


voltage, which inhibits the neuron activity, preventing SNNs from over-activation. Such that additional tuning methods such as weight-thresholds balancing [@diehl2015fast] is not necessary.

Above equations reveal the filter nature of the biologically realistic neuron model. Each synapse act like a low pass filter. Synapse filter is causal, and the kernel is defined to decay over time, hence the current state of the PSP is determined by all previous input spikes up to current time. The temporal dependency calls for temporal error propagation in the training.

Neuron and Synapse as IIR Filters
=================================

In practice, for computational efficiency, spiking neural network are usually simulated in discrete time domain and network states are evaluated for every unit time. The discrete time version of equation \[eq:srm\] can be written as:

$$\label{eq:discrete_srm}
\resizebox{0.91\linewidth}{!}{$
    \displaystyle
 10 different stored spatial temporal patterns at the output indicates that it is capable of learning input features and embedding them into temporal spike structures.

Vision tasks
------------

We trained and tested our method on three vision datasets. Results and comparisons with state-of-the-art works in the SNN domain are shown in Table \[tab:vision\_tasks\]. For MNIST, we utilize rate-based encoding to convert the input image into 784 spike trains where number of spikes in the spike train is proportional to the pixel value. With a convolutional SNN with the structure 64C3-32C3-32C3-256-10, our model achieves state-of-the-art accuracy in the SNN domain. The work next in terms of accuracy (99.42 %) [@jin2018hybrid] employs ensemble learning of 64 spiking CNNs. Compared to conversion-based approaches that require hyper-parameter search and fine tuning [@diehl2015fast], our approach does


Snow on the hills overnight. A fine day promised, but
storms are on the way for tomorrow.

Just one day earlier than I saw the first snow on the hills last
year, though in both cases there may have been some that I missed.
Undeterred by the advancing season, the Yarrow is still flowering profusely,
both the white form and this pale mauve variety.

Was in search of more fungi before the frost hits the lowlands.
I'm afraid we're back to mostly unidentified fungi after Saturday's bonanza.

This orange Hygrocybe can only be the Meadow Waxcap Hygrocybe
pratensis var pratensis, but there are problems with this. The cap
seemed slightly wet or viscid even a day after picking, by which time "dry"
species have invariably dried out. Also it seems very small for H
pratensis although of course these arestudied in vitro separately and in co-cultures. Aim 1 will determine if increased ischemic sensitivity of male vs. female astrocytes and neurons is due to the effects of testosterone-induced reprogramming of astrocytes during brain sexual differentiation. Hypotheses are that manipulation of testosterone levels during brain sexual differentiation will reverse sex-specific sensitivity to OGD in astrocytes and neurons. Aim 2 will determine if testosterone-induced reprogramming of astrocytes during brain sexual differentiation increases cellular (astrocyte and neuronal) ischemic sensitivity by altering expression of the miR-29 family and its target genes, de novo DNA methyltransferases 3A and 3B (DNMT3A and 3B), in astrocytes. Hypotheses are that exposure to testosterone during brain differentiation leads to increased astrocyte and neuronal sensitivity to OGD via reduced miR-29 family expression and consequent increased DNMT3A and


People, animals flee floods in NE Minnesota city

By the Associated Press | June 20, 2012 | 10:05 AM EDT

An automobile navigates the high rushing water on the 200 block of East Seventh Street in Duluth, Minn., on Tuesday evening, June 19, 2012, after heavy rains hit the area. Floods fed by a steady torrential downpour forced road closures in northeastern Minnesota on Wednesday, and some people were being urged to evacuate their homes because of the rising St. Louis River. Interstate 35 and downtown tunnels in Duluth were closed and police recommended emergency travel only, warning that numerous sinkholes and washouts were making travel dangerous. (AP Photo/The Duluth News-Tribune, Clint Austin )

DULUTH, Minn. (AP) — Floods fed by a steady torrential downpour have forced residents to evacuate theirhydrodynamically (-50 mbar, 3 s) using the short-end injection procedure. The electrophoretic system was operated under constant voltage of 30 kV with positive polarity on the injection side. The separation time for hippuric acid, mandelic acid, and creatinine was less than 70 s. The evaluation of some analytical parameters of the method for the three analytes showed good linearity (R 2 > 0.99), limit of detections of 0.21 to 0.63 mg L-1, inter-day precision better than 3.0% (peak area), and recovery in the range of 98 to 106%. The method developed was applied in the analysis of the three analytes in urine samples. Graphical Abstract New method using capillary zone electrophoresis for analysis of creatinine, hippuric acid and mandelic acid in urine.


playing their first show with their new line-up the same time I was back in NY for a buddy's wedding; the timing was too good. No two ways about it, the Metal Gods planned for this to happen.

Boston pretty much invented metalcore with Overcast and Shadows Fall, so it's understandable why Swarm of Eyes may hold breakdowns closer to their hearts than the rest of us. When they ditch the metalcore tics, they start to resemble something akin to a gnarlier Evocation, and are all the better for it. They've got the chops if not the big ideas, and with their laconic charm and New England humour, their time on stage is enjoyable.

It feels like Grey Skies Fallen have been around forever, and for the life of mebe on the way out...possibly because of things like this?). I was looking forward to seeing Mortals; just my luck the only show they played while I was in town was the same night as Gwynbleidd. Hopefully the next time I'm in town if Mortals and Gwynbleidd play the same night, it'll at least be at the same venue.

I missed the first two bands but luckily arrived in time to catch headliners Woe. At times their stripped down assault takes on an almost ENT-like urgency, black metal and power violence coalescing into one fast paintstripping assault. The list of bands doing the ultra nekro early Darkthrone thing is ever growing (strangely, Darkthrone themselves are no longer on that list) but these guys do it with convincing vehemence. Plus,


could feel alive." "Mornings you start at 11 and now afternoons at 4!" "It's too hot!" "But I work till dark, lots of hours!" "That's the end of that!" "Who are you to talk to him like that?" "I'm not talking to you!" "Guess who you are talking to?" "The greatest writer alive, fat ass!" "Doesn't show on his face!" "And she hasn't even got panties on!" "Take a good look, schmuck!" "Shut up, you!" "Now get your eyes full!" "She's a real hellcat!" "Not hurt, are you?" "You asshole!" "Now get rid of that girl!" "I don't ever want to see her again!" "I think I'll go to work now." "Hey, Betty, I'm going to work." "She's in a real frenzy!" "She loves housework!" "There goes the""I return this nauseating flower you call a novel." ""Rely on me for publicity." ""Leave that thing where it belongs..." ""in the quagmire of your brain." ""Sincerely yours, Thomas Colas."" "That's just part of the game." "We're bound to run into morons." "It won't kill us." "It's not important, my love." "OK, it's not important." "I'm going to a doctor." "Anything wrong?" "No, just to see if my IUD is OK." "Coming with me?" "I'll put some make-up on first." "I love to look at old magazines, it's reassuring." "I look silly, give me my jacket!" "But I look good in it!" "Is this it?" "There's no plaque!" "Stop it, he's not deaf!" "My wife has an appointment." "Did you write this?" "You recognize this, don't you?" "Who


"Don't let them get you down!" "When I think of those assholes ruining a year's work in 5 minutes." "I can't say your friend's right, but there again she's not wrong." "A good cop story full of sex!" "That's the best kind." "When that asshole called, I thought: he'll pay for the rest." "I was glad." "I had a drink to celebrate." "She only scratched him." "He's making a mountain of a molehill." "8 stitches." "I'd have demolished him." "Do they think they rule the world?" "Fancy one for the road?" "Your book's sitting here nice and warm." "Like a plane ready to take off." "Believe you me, it'll be published." "I don't think so." "It will, I'm sure." "What about Betty?" "Can we drop the whole thing?""Isn't it an insult to let a silly little barrier bug us?" "OK, but go easy now." "Don't worry, we're not crazy." "All right, bye!" "Do I look like Stallone in Rocky IV, baby?" "No, like you writing your book." "I don't see what demolition has to do with writing." "I'm not surprised." "Want to go shopping with me?" "Do you want to?" "I'll go alone then!" "What's up?" "I'm warming my ass." "I've bought you some popcorn." "Give me the keys." "I'll drive." "Fucking gear-box!" "Sure, blame the gear-box." "Try a bit harder, we'll set a new accident record." "I won't get in that goddam crate again." "You're acting like shit today!" "You're out of your mind!" "Calm down!" "Stop!" "Shit, Betty, stop!" "Betty, please!" "What's going


number 1 in business:" "get yourself noticed." "Rule number 2:" "have a great catch-line." "Once in a lifetime sale!" "Right, I'll go and wash, then get lunch ready." "It already looks better, huh?" "Oh no, another 2 pounds!" "Breathe in." "A closed piano is a dead piano." "I sell live pianos, guys." "Live ones!" "Look at all your little teeth sparkling away." "You have to come alive, today of all days." "Do it, for my sake." "I'm sure you'll make a great team." "It's ready!" "Are you coming up?" "Yeah, I'm coming." "I'm counting on you." "And let's not let her distract us today, okay?" "See you later." "You know, sweetheart, we could eat in the kitchen." "Diet today." "You're right, it has more flavor." "Coffee?" "No, Ihot pants and yours is going bananas." "Don't ever say that!" "I'm sorry." "Aren't you glad to see Eddy and Lisa?" "And Rusty?" "Even bald I'd love you!" "Life is against me." "If I want anything, it's denied me." "I can't even manage to have a baby." "What's wrong?" "Your hair looks fine!" "You should swim a bit." "The water's just right!" "You're insane!" "Watch the float and you don't have to worry." "I don't give a fuck." "I don't want to be a nuisance but is Betty all right?" "She's lost her color." "She keeps on biting her lips and looking at the stars." "Fuck, you're worse than a fish, huh?" "Can't I help you out?" "She thought she was pregnant." "Well, it can't work every time."


Q:

Bound on the relative measure of $\delta$-neighbourhoods of compact sets using the Hardy-Littlewood maximal theorem

Notation: $|A|$ is the Lebesgue measure of $A \subset \mathbb{R}^d$, and $A_\delta = \{ x : \text{dist}(x,A) \leq \delta \} $ is the $\delta$-neighborhood of $A$.
I want to show that there is a constant $C$ such that $|E_{2\delta}| \leq C |E_{\delta}|$ for all compact sets $E \subseteq \mathbb{R}^d$ and all $\delta > 0$.
I have been told I need to apply the Hardy-Littlewood maximal theorem to $f = \mathbb{1}_{E_{\delta}}$.  For $p > 1$, this gives the estimate $||Mf||_p^p \leq C ||f||_p^p = C|E_{\delta}|$.  For $p = 1$, it is the estimate $\lambda \cdot |\{x : Mf(x) > \lambda    \}| \leq C ||f||_1 = C |E_{\delta}|$.  Here
$$
Mf(x) = \sup_{r >in the media was measured after 0 h, 24 h, 48 h, and 72 h. As shown in [figure 2](#pone-0016284-g002){ref-type="fig"}, increasing the MOI above 1 did not result in increased expression levels of NA. All constructs but construct 1 showed strong NA activity in the media ([Fig. 2](#pone-0016284-g002){ref-type="fig"}) as well as strong signals in the corresponding anti-FLAG western blots ([Figure S1](#pone.0016284.s001){ref-type="supplementary-material"}). As construct 1 showed only negligible NA activity and low expression levels it was not further pursued.

![Optimizing MOI.\
To optimize the MOI, Sf21 cells were infected with all 4 constructs at a MOI of 1 (white bars), 2 (light gray bars), 3 (dark gray bars) and 4 (black bars). NA activity in the media was measured at 0 h, 24 h, 48 h and 72 h. Increasing the


NA over the time was monitored via its activity to ensure optimal yields (data not shown). In parallel, media samples were taken to track expression of construct 4 by PAGE and western blot ([Fig. 5A, B](#pone-0016284-g005){ref-type="fig"}). Although low in sensitivity, UV imaging of the gel ([Fig. 5A](#pone-0016284-g005){ref-type="fig"}) subsequently used for anti-FLAG western blot ([Fig. 5B](#pone-0016284-g005){ref-type="fig"}) showed that construct 4 was detectable. Anti-FLAG affinity chromatography, as an initial purification step, was already sufficient to purify the enzyme to virtual homogeneity as supported by the Coomassie stained gel ([Fig. 5C](#pone-0016284-g005){ref-type="fig"}) and the respective western blot ([Fig. 5D](#pone-0016284-g005){ref-type="fig"}). In addition, whereas the Coomassie stained gel showed the presence of a variety of proteins in the insect cell media ([Fig. 5C](#pone-0016284-g005){ref-type="fig"}; lane Flowthrough) the western blot indicated that all FLAG-reactive protein wasexcess substrate immediately after purification. [Figure 6](#pone-0016284-g006){ref-type="fig"} shows the accumulation of fluorescent product over time for constructs 2 and 4. Based on the linear slope of both curves (FUs/sec), a 4-Methylumbelliferone (MU) standard, and the concentration of the enzyme in the assay, a specific activity of 387±26.7 and 377±12.7 pmol/sec/µg was calculated for construct 2 and construct 4 respectively ([Table 1](#pone-0016284-t001){ref-type="table"}). To test whether the purified enzymes show comparable characteristics to the original viral NA, the *K* ~m~ value for MUNANA as well as the IC~50~ values for the NA-inhibitors were determined. To calculate the IC~50~ values the Hokkaido virus and both recombinant enzymes were pre-incubated with increasing concentrations of NA inhibitors, oseltamivir, zanamivir and (1S,2S,3S,4R)-3-\[(1S)-1-acetamido-2-ethyl-butyl\]-4- (diaminomethylideneamino)-2-hydroxy-cyclopentane- 1-carboxylic acid (peramivir). The maximal observed catalytic rate for each reaction


presence of oseltamivir (B), zanamivir (C), or peramivir (D). Data represents mean ± SEM of 4 independent experiments performed in duplicate.](pone.0016284.g010){#pone-0016284-g010}

Discussion {#s3}
==========

Despite seasonal influenza being responsible for a world-wide death toll of more than 250,000 per year, the availability of anti-viral therapies and vaccines have reduced the public concern about this common viral disease. However, this dramatically changed with the rapid spread of 'swine flu' and the world-wide emergence of the Tamiflu-resistant seasonal A(H1N1) H~274~Y human influenza. In the light of these incidents it is evident that understanding the emergence of resistance, the need to quickly develop sufficient doses of vaccine, and the development of new antiviral drugs are vital for the effective control of influenza. These goals would be greatly facilitated by the availability of larger amountsstalk by more stable and rigid domains that are known to form parallel homotetrameric structures could promote greater NA stability. This approach was first formulated by Fiers et al. for influenza NA[@pone.0016284-Fiers1] and its feasibility to promote homotetramerization has been shown for proteins such as the influenza M2 [@pone.0016284-DeFilette1] or human p53[@pone.0016284-Waterman1]. These publications made use of the yeast transcription factor GCN4 as a heterologous domain to promote tetramerization. Natural GCN4 forms parallel homodimers, however slight modifications of the residues in the dimer interface result in the stable formation of homotrimers or tetramers making this protein a very versatile tool[@pone.0016284-Harbury1]. As GCN4 has been shown to successfully promote tetramerization it was used as an artificial stalk for construct 1 and 2 described in this work. In general, domains


both recombinant enzymes, especially construct 2, showed increased IC~50~ values for all 3 drugs. These findings were supported by the *K* ~i~ values derived from the enzyme activity in the presence of constant NA inhibitor. The inhibitory constant takes into account the decreased affinity for the substrate MUNANA in the absence of drugs making the *K* ~i~ values a more reliable comparison between the original Hokkaido virus NA and the two recombinant enzymes. The *K* ~i~ values for all three inhibitors for construct 4 were close to the ones obtained for the original virus NA, whereas construct 2 showed generally higher values. These results, in combination with the observed higher *K* ~m~ value for construct 2, indicate that the replacement of the natural NA stalk by the yeastoriginal NA stalk was amplified using the primers Hokkaido-Stalk/Hokkaido-Head as shown in [Table 2](#pone-0016284-t002){ref-type="table"}. The sequences for the artificial stalks including FLAG-tag were synthesized by GeneART (Regensburg, Germany). The artificial stalks were fused to the NA head via PCR using the relevant construct primer shown in [Table 2](#pone-0016284-t002){ref-type="table"}. The PCR products were cloned into the transfer vector pFastBac downstream of the coding sequence for the MSP responsible for the secretion of the expressed protein[@pone.0016284-Tessier1]. As a control for some experiments, a baculovirus encoding the full length Hokkaido NA including the natural stalk as well as the transmembrane domain was generated. In the absence of a secretion signal the respective NA was expressed as membrane bound enzyme. Each DNA sequence was verified by sequencing (Micromon, Victoria, Australia). Bacmids and


MU (excitation: 355 nm, emission 460 nm) in black 96 well plates (PerkinElmer, California, USA) for 60 min at 37°C. Fluorescence was recorded using a Fluostar OPTIMA plate reader (BMG Labtech, Mornington, Australia) and data was subsequently analysed using MARS data analysis software (BMG).

### pH sensitivity {#s4g1}

To determine whether low pH has an impact on the activity of the recombinant NAs 50 µl of constructs 2--4 in SF 900II media were diluted ^1^/~20~ in glycine buffer pH 2.8 \[200 mM\] followed by subsequent neutralisation with Tris buffer pH 8.7 \[1 M\] after 1 min, 15 min, and 30 min. The remaining NA activity was determined by standard MUNANA assay as described above.

### IC~50~ determination {#s4g2}

In order to determine the IC~50~ values for zanamivir, oseltamivir and peramivir for theeach enzyme (FU/min) was calculated from three different enzyme concentrations. A MU standard was included to allow the conversion of FU into molarities.

### Kinetic parameters {#s4g4}

In order to determine the *K* ~m~ values for the original virus NA and the recombinant soluble NAs, the enzymes were incubated with increasing concentrations of MUNANA (3 µM--600 µM) in the absence and presence of the NA inhibitors zanamivir, peramivir, or oseltamivir for 30 min at 37°C. The enzyme reaction was started by adding substrate-buffer and the reaction was monitored for 60 min at 37°C as described above. Data was analysed using MARS data analysis software (BMG). The highest observed enzyme rate (FU/min) was plotted against the substrate concentration. The *K* ~m~ values for MUNANA as well as the *K* ~i~ values


[Anti-angiogenesis targeting drugs: a review].
The dependence of tumor growth and metastasis on blood vessels makes tumor angiogenesis a rational target for cancer therapy. One of the key mediators of angiogenesis is vascular endothelial growth factor (VEGF), which is an appealing target for anticancer therapy. The development of anti-VEGF/VEGFR agents and the latest clinical data are reviewed, including bevacizumab as a monoclonal antibody to VEGF, sunitinib and sorafenib as VEGFR tyrosine kinase inhibitors, and IMC-1C11 as VEGFR monoclonal antibody.cleave multiple antigen molecules, and their epitope specificity requirements are less strict than ordinary Abs, as inactivation of gp120 can occur even when cleavage occurs at sites remote from the receptor binding sites of the protein. Induction of the synthesis of catalytic Abs to gp120 has become feasible with the development of electrophilic analogs of gp120 and synthetic gp120 peptides. Abs to these analogs combine noncovalent gp120 recognition with a serine protease-like activity, resulting in specific cleavage of gp120. We propose to study as immunogens the analogs of full-length gp120, whole virus particles and a synthetic gp120 peptide. The elicited Abs will be studied in polyclonal and monoclonal form for their catalytic efficiency, cleavage specificity, ability to recognize native gp120 expressed on the viral surface and neutralization of


Tax tips for a tough year

February 15, 2009 12:28:44 PM PST

WASHINGTON --

How to avoid audits, get the most on your return and what to do if you can't payIn tough financial times, getting taxes right the first time is more important than ever and as some of Obama's Cabinet appointments have proved, slighting the tax man can have devastating consequences.

Luckily, Mellody Hobson, "Good Morning America's" financial contributor and president of Ariel Investments, has some tips to help you avoid audits, collect on your return and survive this tax season.

Be Careful! There Are More Tax Collectors Than Ever The federal government has been increasing audits for the last decade.

The number of people audited more than doubled in that time. And tax experts say there is an even bigger pushLady Frances Russell, who became a Unitarian at 70, and her grandson, Bertrand Russell, Unitarian until age 15, were members of a British family long prominent in reform politics. Although Lady Frances's husband, Lord John Russell, was never a Unitarian, from 1859-73 he regularly attended the preaching of James Martineau and, in his Whig (Liberal) political career, he had a considerable impact on the history of Unitarianism in Britain. Bertrand Russell, after he had rejected Unitarianism, influenced many Unitarians, particularly Humanists. He was president of the Rationalist Press Association, 1955-70, and belonged to the British Humanist Advisory Council. Bertrand's uncle, Francis Russell, wrote Unitarian hymns and verse.

Lord John Russell, later Earl Russell (1792-1878) was throughout his political career an ardent reformer and promoter of civil and religious liberty.


photometric redshift is reported by two quantities, `ODDS` (measuring the spread in the probability distribution function, $P(z)$) and modified reduced $\chi^2$ (measuring the goodness of fit)[^2]. We require the photometric redshift sample to have `ODDS`$>0.9$ and modified reduced $\chi^2<1$ to ensure selecting only sources with reliable photometric redshifts. Applying these cuts gives a sample of 234, 258 and 440 galaxies in the redshift ranges $1.4<z<1.9$, $1.8<z<2.6$ and $2.4<z<3.6$, respectively.

Using the photometric redshifts enables sample selection down to fainter magnitudes than the corresponding dropout criteria. The dropout selected samples require $5\sigma$ in the detection band to confirm the strength of the break. On the other hand, photometric redshift selected samples only require a $5\sigma$ detection in the rest-1500Å filter. At these redshifts, the dropout detection band (F275W for F225W dropouts,function of apparent magnitude and galaxy size, which is used to define the survey magnitude limits (see Appendix \[appndx:comp\]).

![image](f2a.pdf){width="33.00000%"} ![image](f2b.pdf){width="33.00000%"} ![image](f2c.pdf){width="33.00000%"}\
![image](f2d.pdf){width="33.00000%"} ![image](f2e.pdf){width="33.00000%"} ![image](f2f.pdf){width="33.00000%"}

Selection Functions {#sec:self}
-------------------

The mock galaxy sample can be used to determine the probability of a galaxy to satisfy the LBG selection criteria as long as there are no biases in the recovered magnitudes and colors of the mock galaxies. We verify that our completeness simulations do not introduce any offsets or biases in the magnitudes and colors of recovered mock galaxies, before proceeding. We apply the LBG selection criteria to the input mock sample to quantify the relative efficiency of the criteria to select a galaxy with given redshift, rest-1500Å absolute magnitude and half-light radius. The top row in Figure \[fig:self\] shows the selection function for the three dropout


from Equation \[eqn:sch\], $M_{lim}(z)$ represents the survey’s detection limit, $v_{\mathrm{eff}}(M,z)$ is the effective differential comoving volume and $N(M|\{M_i,\sigma_i\})$ is the term that marginalizes over the measurement error in the galaxy’s magnitude. The effective differential comoving volume is defined as:

$$\label{eqn:veff}
v_{\mathrm{eff}}(M,z) =  \frac{dV_{comov}}{dz \ d\Omega}(z) \cdot S(M,z,r_{1/2}) \cdot \Omega \\$$

where $S(M,z,r_{1/2})$ is the selection function, which defines the efficiency of the selection criterion at a given redshift $z$ as a function of the absolute UV magnitude $M$ for a given half-light radius $r_{1/2}$ derived from the completeness simulations in Section \[sec:comp\], and $\Omega$ is the solid angle surveyed. The measurement error associated with the absolute UV magnitude ($\sigma_{M,i}$) is modelled as a Gaussian:

$$\label{eqn:gauss}
N(M|\{M_i, \sigma_i\}) = \frac{1}{\sqrt{2\pi}\sigma_i} \mathrm{exp} \left[ - \left( \frac{(M - M_i)^{2}}{2 \sigma_{M,i}^{2}}\right)\right]$$

In the MLE formalism, the Schechter function normalizationof the star-formation rate, instead of luminosity. Converting the LFs to SFRFs requires transforming the luminosities into a star-formation rate. As before, we correct the UV LF according to typical dust prescription, the M99 $IRX - \beta$ relation (expanded to a $IRX - \beta - M_{UV}$ relation), and the [H$\alpha$]{} LF according the @hopkins01 relation adjusted using @dominguez13 results. Both UV and [H$\alpha$]{} dust-corrected LFs are then converted into SFRFs, using the transformation tabulated in @kennicutt12 (which quotes @murphy11). [^4].

Figure \[fig:SFRF\] shows the resulting $z\sim2$ SFRFs, with the SFRF calculated using M99 shown in *red* and the SFRF from [H$\alpha$]{} shown in *black*. The comparison between these two estimates shows that most of the discrepancy originates at the bright end. For SFR $>$30 M$_\odot$ yr$^{-1}$, the [H$\alpha$]{} SFRF estimates for a factor of


on the recent star formation history. Figure \[fig:uv\_vs\_ha\_ratio\] shows the evolution of the UV-to-[H$\alpha$]{} ratio for a variety of star formation histories. The [H$\alpha$]{} output from a galaxy drops on much shorter timescales, after the end of a burst, compared to the non-ionizing UV, which takes longer to react, because of the longer lived B and A stars that still produce UV photons, but have very little ionizing output. For a constant star formation history, the rates at which massive stars are formed and die reach an equilibrium after approximately 100 Myr, and therefore the UV-to-[H$\alpha$]{} ratio tends to a constant value (log$_{10}[\nu L_\nu (1500)/L_{H\alpha}] \sim 2$); whereas, a burst of star formation would cause the UV-to-[H$\alpha$]{} ratio to scatter towards higher values.

Figure \[fig:uv\_vs\_ha\_ratio\] shows how the UV-to-[H$\alpha$]{} ratio evolves for [@bc03] models with [@salpeter55],extrapolated.[]{data-label="fig:uv_vs_ha_mass"}](f8.pdf){width="45.00000%"}

[ccccccccc]{}

[@meurer99] & 1.46e-04 & -2.13e-05 & -2.49e-03 & -6.37e-03 & 2.21e-03 & 1.41e-01 & 1.95 & 0.154\
[@reddy15] & 1.46e-04 & -1.19e-05 & -2.51e-03 & -6.60e-03 & 1.92e-03 & 1.46e-01 & 2.06 & 0.153\

[@meurer99] & -7.61e-03 & 5.58e-02 & -1.42e-01 & 1.28e-01 & 5.19e-02 & -2.99e-01 & 2.01 & 0.154\
[@reddy15] & -7.67e-03 & 5.66e-02 & -1.44e-01 & 1.31e-01 & 5.28e-02 & -3.10e-01 & 2.12 & 0.153\
\[tab:emp\_fits\]

Conclusions {#sec:conclusions}
===========

NUV coverage of the *Hubble* UDF provided by the UVUDF enables for LBG dropout and photometric redshift selection of galaxies near the peak of cosmic star formation ($z\sim2-3$). Additionally, it also enables the study of their rest-frame UV properties and consequently, their star formation properties. Here, we present the rest-frame 1500Å UV LFs for F225W ($z\sim1.65$), F275W ($z\sim2.2$), and F336W ($z\sim3$) dropout galaxies in


under NASA contract NAS5-26555. M.R. also acknowledges support from an appointment to the NASA Postdoctoral Program at Goddard Space Flight Center. M.B.K. acknowledges support from the National Science Foundation (grant AST-1517226) and from NASA through HST theory grants (programs AR-12836, AR-13888, AR-13896, and AR-14282) awarded by the Space Telescope Science Institute (STScI), which is operated by the Association of Universities for Research in Astronomy (AURA), Inc., under NASA contract NAS5-26555.

Completeness Functions {#appndx:comp}
======================

From our simulations, the completeness functions are computed using the fraction of recovered sources as a function of observed magnitude as well as galaxy size. We use the B$_{435}$ half-light radius as a proxy for the galaxy size. These completeness functions are marginalized over all sizes to obtain completeness just as a function of magnitude, which isSymmetrical necrosis of globus pallidus with severe gait disturbance in a patient with myelodysplastic syndrome given allogeneic marrow transplantation.
A 21-year-old Caucasian man received an allogeneic marrow transplant (BMT) from his HLA-identical brother because of myelodysplastic syndrome. He remained red blood cell (RBC) transfusion dependent with persistent antibodies against the donor's RBC. Six months following BMT the patient suddenly developed a severe akinetic syndrome with gait disturbance and frequent falls and bilateral symmetrical lesions in basal ganglia. Concomitantly, micrococcus species septicemia from an infected Hickman catheter developed. Despite antimicrobial therapy and withdrawal of cyclosporin A, neurologic abnormalities persisted and were unresponsive to various therapies. Ischemic damage due to a vascular event during severe infection could be the most probable reason for the lesions seen in our patient, although infectious


low emphasis
on these strategies were high performers.

"Manufacturing often takes a subordinate role in firms

because more concentration is placed on what the company can
sell rather than what it can produce," said Peter Ward, co-author
of the study and assistant professor of management science at
Ohio State University's Max M. Fisher College of Business.

"But successful firms don't ignore manufacturing; they
are pro-active in making manufacturing decisions."

Ward conducted the study with G. Keong Leong, an associate
professor of management science, and Kenneth Boyer, a graduate
student, both at Ohio State. Their study was published in a recent
issue of the journal Decision Sciences.

The study involved manufacturing firms that had plants located
in Ohio with at least 150 employees. The firms' primary product
was in one of five industries: hand tools, fabricated metal products,
plating and polishing, current carrying wire devices,firm.

While good communication is important, it is not enough to
make a company a top performer, Ward said. "It takes more
than just listening to what manufacturing executives say. There
has to be an investment in technology."

The results also emphasize the importance of investing in
employees. "Successful technological programs also require
significant investments in people," he said. "For example,
employees need to be trained to properly use new technology when
it is introduced in the workplace."


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                conn.Open();
                comm.ExecuteNonQuery();
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    using (MySqlCommand comm = new MySqlCommand(sqlString, conn))
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        comm.Parameters.Add("@ProdID", MySqlDbType.VarChar).Value = prod.ProdID;
        comm.Parameters.Add("@Name", MySqlDbType.VarChar).Value = prod.Name;
        comm.Parameters.Add("@Color", MySqlDbType.VarChar).Value = prod.Color;
        comm.Parameters.Add("@Availability", MySqlDbType.VarChar).Value = prod.Availability;
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}

I have created a fiddle to demo


It’s always fun to play “guess the unintended consequences” whenever some new idiocy from Washington D.C. surfaces. The Cash for Clunkers program (CFC) will give between 3500 and 4500 dollars for cars with certain conditions (like low gas mileage) when they are traded in for new cars with better gas mileage.

But since the program is going through it’s important to note what problems it will cause. Here are my predictions as the program evolves:

1) A great number of cars not being used regularly will be turned in for the money. These are tertiary vehicles for people who rarely use them and taking them off the street will do no good since they are, well, rarely used.

2) Poor people will try to profit on their cars. People who driveit.

However, Knobloch-Westerwick added that her latest study was not designed to assess reader motives, and that she hopes to more carefully study the issue in the future.

D’oh. Didn’t have the numbers to fudge those conclusions, so you just made some stuff up. Good call.

It’s hard to seperate the bias of the reporter from the bias of the study authors, but you get the point. I’ve exaggerated the completely made up conclusions about conservatives, not much though.

Interesting mix. Rocco is unimpressive, but Yaz is very very impressive. And Clint Hurdle? That’s just horrible.

Considering all the information available, Delmon Young skeptics are being too skeptical. Of course, friends who gush about Delmon Young being the future of the franchise (and there are several) are probably in for a terrible surprise.


of the Virus

The plantar wart is often contracted by walking barefoot on dirty surfaces or littered ground where the virus is lurking. The virus thrives in warm, moist environments, making infection a common occurrence in communal bathing facilities.

If left untreated, warts can grow to an inch or more in size and can spread into clusters of several warts; these are often called mosaic warts. Like any other infectious lesion, plantar warts are spread by touching, scratching, or even by contact with skin shed from another wart. The wart may also bleed, another route for spreading.

Occasionally, warts can spontaneously disappear after a short time, and, just as frequently, they can recur in the same location.

When plantar warts develop on the weight-bearing areas of the foot—such as the ball ofyou write a book, it’s so. much. easier. I AGREE. It’s a lifesaver. Plus when I need to bounce ideas off my agent it’s better to have a flexible synopsis instead of a stammering email of “Sooooo. I like dragons and I want them to eat people and probably they cough up icicles instead of fire?”

I usually write 2,000-3,000 words! I outline scenes and basically have an outline of events. I definitely make sure I have a beginning and an ending. I make sure I have a VERY detailed middle because middles are easy to lose interest and decide to abandon authorhood and take up professional penguin training.

If I get stuck? If the plot just isn’t coming? If I despair the plotting will ever come together? I JUST


and not get bored. (Not only do I stick up my plots, characters, timelines, etc. but origami, quotes, French verbs, posters, a world map… the list goes on.) Plus my family thinks I’m insane, but whatever. Thanks for sharing your writing process!

Oh, Cait – I so love all of this. I think I need to take your advice here because I tried writing my NaNo book without plotting last year, and it was … just … well … plotless. I LOVE the characters and I think I have a solid idea, but the plot just didn’t go much of anywhere. I’m not giving up on it yet – I’m still planning to wrestle the book into submission, but I realized I needed to do some serious plotting andand then I see how things go from there xd But for my nano novel this year which is a fantasy (something I have never tried writing before!) the world came first, then the plot and lastly the characters so I am feeling a bit out of depth and lost but I will write it anyway! I love how your novel ideas and writing changes entirely as the months go by 😀

AHHH I ENVY YOUR CHARACTERS THEN. XD I almost always have this great plot and world and it’s like…”okay. Characters. Who can I steal from a different book?” XD I think I wrote one character twice in two books this year. Oops. 0_0 Edits here we come…*sigh* OMG WRITING FANTASY IS SO SO FUN!!! Fantasy is like


more interesting. Plus. pictures. Pictures are so underestimated. And yeah, ideas from things that turn into nothing like the original inspiration happens to me all the time. I look back and think, “What happened to you? You used to be a cute, little toddler of an idea and now you’re a monstrous adolescent who eats everything?! Someone help me.”

“You can sleep when you’re dead.”– Who needs sleep? Writing ideas wait for no sleeper! So much truth.

I’m kind of a mixture of a pantser and a plotter. I usually pants through most things. But if my idea is on hold, I tend to plot/mental write. But usually my plotting consists of random, diabolical scenes, a load of world-building, eh. . . and scattered tidbits of hilarious/devastating (or so Isovereign. The whole country was literally their possession. They could just take what they wanted, although in practice they often had an aide pay quietly so their subjects wouldn't hate them. Nobles did carry money, however. History is quite clear on that.

Yes, good post, Kim. It is relative prices that are most informative when trying to understand and describe the past.As for kings/queens never carrying money, I think it had to do with dignity. The royal treasury travelled with the king, and he was usually accompanied by an almoner to give out alms. The king/noble disposed over wealth, but didn't actually handle it or count it. That was for his subordinates/employees to do.

What a timely post ... I'm working on my major historical fiction novel project right now.


a reason for the decreasing number of incidents after 36 month. Eisenburger and Tschernitschek defined two phases after receiving new removable dentures: The first phase, the adaption phase, ends after 24 months, followed by the use phase. They found that the highest number of failures or need for corrections occurred during the adaption phase.[@B13] The findings of the current study might suggest that the adaption phase of FDPs ends within the first 36 month, which also might result in a decreasing number of incidents.

The findings of the current study are in line with current literature on the overall survival of veneered zirconia restorations. In 2015, Beuer et al.[@B14] published 3 year clinical results for veneered zirconia FPDs on teeth or implants. Kaplan-Meier analysis showed survival rate probabilities ofsurvival rates of all-ceramic FPDs. In 2011, Sax et al.[@B21] reported the clinical outcomes of FPDs with zirconia frameworks. They concluded that zirconia frameworks showed good longterm stability (91.5%), but problems such as chipping were frequently reported. They found a mechanical complication rate of 32%, but traced this back to the "prototype status of the system". In 2016, Ioannidis and Bindl[@B22] published 10 year results of a clinical evaluation of 57 veneered zirconia-based three-unit FPDs. They found technical failure rates of 28%. The CSR after ten years was 85%. In total, only three (5.2%) of the 57 restorations failed because of biological complications.[@B22] The data of the current study showed overall 5 year survival rates of porcelain veneered all-ceramic restorations of about 93.4%. However, further research is needed


Fatty infiltration of the peripancreatic space: CT confirmation of a normal variant.
Widening of the duodenal sweep on upper GI series is a sign of mass in the pancreatic head. A series of patients was examined in which the widening of the duodenal sweep was solely due to fatty infiltration of the peripancreatic space. Because CT established the diagnosis, more invasive procedures were obviated..addClass(), support custom durations and easings, as well as provide a callback for when the animation completes.

Not all styles can be animated. For example, there is no way to animate a background image. Any styles that cannot be animated will be changed at the end of the animation.

This plugin extends jQuery's built-in .addClass() method. If jQuery UI is not loaded, calling the .addClass() method may not fail directly, as the method still exists. However, the expected behavior will not occur.


that way.

Ever notice how a fresh basil leaf makes everything look that much more healthy?

I wanted this recipe to be really easy, so I just used pre-formed sausage patties I found at the store.

You can also chop up the mushroom stems you pop out of the caps and incorporate them into the sausage ball mix, or eat them plain. They’re pretty delicious! (I saved my stems for a different recipe I’ll share soon).

Quick Microwave Stuffed Mushroom Caps

2 patties sausage

12 mushroom caps, rinsed and wiped clean

2 Tbsp flax seed meal

1/2 tsp parsley

1/4 tsp salt

1/4 tsp pepper

4 Tbsp shredded Parmesan cheese

Combine sausage with flax seed meal, parsley, salt and pepper in a small bowl (you can even use the bowl you’ll use to cover the mushrooms while cooking). Form sausagewe take a look at some of the key stories from the game...

Selection

Arsene Wenger might have been expected to rest some of his key players given that the Gunners came into the home leg with a three-goal lead and the north London derby awaits at the weekend, but instead it was a strong line-up for the crowd at the Emirates Stadium to enjoy.

Bacary Sagna was preferred to Laurent Koscielny in the centre of defence but other than that this was perhaps the best team Wenger could name, featuring as it did the same midfield and striker that produced such a convincing performance in the win at Fulham on Saturday.

Fenerbahce made six changes to the team that surrendered so meekly in Turkey, including three of the back four. Perhaps


Incorporation of anionic lipids (phosphatidylserine) into the vesicles did not increase the binding affinity nor did altering the ionic strength. These experiments suggested that the interactions are primarily driven by hydrophobic effects not ionic effects. Using vesicles doped with either 5-doxyl phosphatidylcholine, 10-doxyl phosphatidylcholine, or phosphatidyl-tempocholine, quenching of acrylodan fluorescence was observed upon binding. Using the parallax method of London [Chattopadhyay, A., and London, E. (1987) Biochemistry 26, 39-45], the acrylodan ring is calculated to be 8.1 +/- 2.5 A from the center of the lipid bilayer. Modeling the acrylodan-cysteine residue as an extended chain suggests that the backbone of the loop does not penetrate into the lipid bilayer but interacts with the headgroups, i.e., the choline. These results demonstrate that cholesterol oxidase interacts directly with the lipidaffiliate program with that company? Also, if it is products with a company, then how do I offer promotions on their products since they are not mine? Thank you, Nanette Vlahusich

It may take time to build that audience that turns views into dollars. The average revenue per 1,000 YouTube views is just over $6. But with enough videos for fans to scroll through, those views can add up over time. While you're building an audience, you could also join an affiliate program related to your channel and make money through affiliate links in your video descriptions.

29. Videos – This could be an entire section on it’s own. Many people have made money by creating YouTube videos. Evan of EvanTube is a kid and he has made millions by


Be sure that you set your expectations the proper way. As long as you stay persistent, you can achieve your goals of generating healthy amounts of money online without becoming the victim of a scam.
Now you’ve got many different options to start earning online. If you saw something that really interests you, try it out and learn more about it. If you’re really wanting to make a full-time income online, you need to be dedicated to learning how to do what you want to do. There are tons of free resources out there. You just have to search for them!
Some good tips. I fully agree that it’s all about trust and it is always good to recommend an affiliate product that you have actually used. Why risk yourseveral arcseconds away from Pluto. Complex structure can be seen in this pattern, as a result of the higher-order behavior in the wings of the ACS HRC point spread function (PSF).

Results
=======

To reduce the strong background gradients in the processed images due to Pluto and Charon, we employ the high-pass filter method of [@Showalter:lissauer06]. At each pixel in the image, a first-order, two-dimensional polynomial (i.e. a tilted plane) is fit to the pixels lying within a surrounding circular region. A small circular aperture at the center of this region is excluded from the fit so as not to reduce the intensity of point features. A radius of 12 pixels was chosen for the outer limit of the fit; a radius of 5 pixels was chosen for the inner aperture,


to enclose 80% of the flux from a point source [@Siriannietal05]. The value of the fit is then subtracted from the central pixel and the process repeated for all pixels in the drizzled images. The resulting filtered images still contain the high-frequency components of the 2-D pattern of flux from Pluto and Charon, but the low-frequency components are much reduced. Visual inspection of the filtered images reveals no immediate evidence for a faint ring system. As noted by [@Showalter:lissauer06], this high-pass filter method is not photometrically accurate (tending to narrow bright features), so an alternative method must be used to place quantitative limits on the I/F of a potential ring system.

Flux from Pluto and Charon is the dominant source of counts in the region of the images whereunimodal ring of 100 $\mu$m particles, up to 2x10$^4$ such collisions could be expected.

Our ring constraints are not tight enough (by several orders of magnitude) to ensure the safety of the [*New Horizons*]{} spacecraft, were it to cross the ring plane at a distance where ring particles might be present. In the absence of tighter constraints on the optical depth of a putative ring system, we therefore recommend that [*New Horizons*]{} cross the ring plane within 42,000 km of the system barycenter, where dynamical interactions with Charon prevent stable orbits [@Sternetal94; @Nagyetal06]. The current planned trajectory for [*New Horizons*]{} is safely within this distance.

Finally, we can use our ring optical depth constraint to estimate the lifetime of ring particles at Pluto. First, we point out that the re-collision timescale for


for himself." "Here, cheese." "I'm not hungry." "Yeah, you will be." "I saw the other americans." "So none of you have called the embassy, huh?" "Us gringos ain't all butt buddies." "You want it?" "'Cause I'll keep it." "Let's get this done." "We're gonna have to watch both guards simultaneously." "Your cell should provide a good view of the guard I saw yesterday." "Keep track of everything he does and when he does it." "Look for that glare, see if it shows up again." "What will you be doing?" "Watching the other tower from here." "While our back's turned, we're gonna need a lookout." "Well, find mahone." "I thought we were stringing him along?" "Looks like we're out of string." "Teodoro." "Si, patrón?" "What they say about allscared." "I was scared." "coward" "Hurtado!" "Ya." "Dejelo." "come on." "Come on!" "Please, please, please." "Come on." "He's coming back up here." "Now, don't worry." "It's all going to be okay." "You have a little scara right there." "You need to look pretty for lechero." "Thank you." "Wait right here." "A patrol passed through, but they didn't stay." "Knocked some things over then walked away." "I hid your woman in a safe place, but I'd be lying if I didn't say it was a close call." "Is she alone?" "Yeah." "Hey." "Don't be afraid." "No, no, my dear." "They're gone." "I should go, too." "No, no, no." "What's the rush?" " Come on." " No, I should get home." "Patr**, I gave her the money." "I was going


to replace it mysf at the end of the day, when I get my cut." "I just didn't get a chance to tell you." "She had no money to get home." "Is this true?" "Yes." "With all due respect." "Surely you don't want your mistress traveling' broke." "She'd end up in a taxi with no way to pay the cab fare." "ah, you did this?" "For me?" "Si, patron." "Teodoro." "The one that does all the useful things for me, huh?" "And now you do the thinking for me." "No, no, it's not like that, patron" " get the bucket." "Wash my feet." "so what do we do now?" "I don't know." "Well, I'm afraid that's not good enough." "Please just give me a moment to think." "WastingMANHATTAN (CN) — Precluding a trial on massive tax dodges unearthed in the Panama Papers leak, an accountant implicated in the scheme pleaded guilty Friday.

Richard Gaffey, 75, had been one of four men arrested after the exposure of 11.5 million files from the Panamanian law firm Mossack Fonseca implicated leaders and luminaries in illegal asset shuffling.

“I apologize to the government and the court for my actions,” Gaffey told U.S. District Judge Richard Berman this morning, admitting to eight criminal charges.

Gaffey’s prosecution began with groundbreaking investigative journalism in 2015.

After obtaining a record-breaking trove of leaked documents, the German newspaper Suddeutsche Zeitung partnered with the International Consortium of Investigative Journalists, Organized Crime and Corruption Reporting Project, and media companies in 70 countries on an investigation showing how the rich stashed


Dike Lake

Dike Lake is an alpine lake in Custer County, Idaho, United States, located in the White Cloud Mountains in the Sawtooth National Recreation Area.  No trails lead to lake but it can be accessed from Sawtooth National Forest trail 680.

Dike Lake is just southeast of the Chinese Wall, northeast of Calkins Peak, and in the same basin as Gunsight, Quartzite, and Tin Cup Lakes.

References

See also
 List of lakes of the White Cloud Mountains
 Sawtooth National Recreation Area
 White Cloud Mountains

Category:Lakes of Idaho
Category:Lakes of Custer County, Idaho
Category:Glacial lakes of the United States
Category:Glacial lakes of the Sawtooth National Forestor Vancouver. The 7-11 in Squamish is located right off the highway next to McDonald’s so it’s an easy pit stop.

Edge card-If you’re a resident of Washington state or Canada, consider getting an Edge card for discounts of up to 20-25%.

Guide Book:

Ski and Snowboard Guide to Whistler Blackcomb, Advanced/Expert Edition by Brian Finestone and Kevin Hodder (Intermediate Edition also available). To find detailed maps and descriptions of the runs I’ve recommended, plus many more, pick up this guidebook at local ski shops, or on Amazon. Most of the runs I’ve listed are double black diamonds. The book lists many triple black diamond runs as well.

Getting There:

To reach Whistler from the U.S., fly to Vancouver International Airport (YVR) in British Columbia, Canada, then rent a car and drive 1.5-2


give it a try.

Definitions and terminology
===========================

Autonomic nervous system
------------------------

When discussing the autonomic nervous system (ANS), pioneering researcher and Nobel prize winner in physiology and medicine, Hess ([@B61]) as well as early researcher Gellhorn ([@B49]) used the terms "ergotropic" (energy seeking) and "trophotropic" (nutrition seeking) to point out that the two principal branches of the ANS cannot be isolated from the somatic and central nervous systems and the neuroendocrine system. The ergotropic system includes activation of the sympathetic nervous system as well as the motor and premotor system (increased muscle tension and preparedness to act), the endocrine system (increased secretions of a number of stress hormones), and the central nervous system (increased sensory alertness), in a coordinated preparation for strong energy expenditure ("fight or flight"). In contrast, the trophotropic systemimmediate, instinctive response to environmental challenges, prior to extensive cortical processing. It includes the autonomic nervous system (hypothalamus), the limbic emotional system (amygdala, hippocampus, septal region), the emotional motor system (portions of the basal ganglia, red nucleus, periaqueductal gray), and the reticular arousal systems. All these systems interact strongly through multiple feed-back and feed-forward connections, forming a complex dynamical system which can enter various discrete functional and dysfunctional states.](fpsyg-06-00093-g0001){#F1}

There is extensive evidence that these four networks interact strongly (Gellhorn, [@B49]; Weinberg and Hunt, [@B145]; Hamm et al., [@B58]; Critchley, [@B25], [@B26]; Thompson, [@B136]; Coombes et al., [@B21]; Hajcak et al., [@B54]; Sze et al., [@B133]; Kim et al., [@B69]; Herbert and Pollatos, [@B60]; Price et al., [@B106]; Norman et al., [@B94]). The ANS can intensify or calm the


activity of the viscera, alter blood circulation, trigger hormonal and endocrine activity, change muscle tone, increase or decrease cognitive arousal, and contribute to emotional experience (Norman et al., [@B94]).

The LS, including amygdala, hippocampus, and septal regions, is central to fear- and pleasure-based experience and to the recall of emotional significance (Heimer and Van Hoesen, [@B59]). This network has strong bi-directional links to the ANS (Uylings et al., [@B137]), and the RAS (Strominger et al., [@B131]), and triggers emotion-specific movement and posture via the EMS (De Gelder, [@B35]). The RAS involves multiple networks which trigger arousal through several different pathways. It controls alertness and orientation in different contexts, and interfaces strongly with LS, ANS and EMS (Krout et al., [@B72]; Berntson and Cacioppo, [@B8]). The EMS involves multiple subcorticalsympathetic and parasympathetic (or more exactly, ergotropic and trophotropic) systems at the other. At precisely what point the stress should be regarded as "traumatic" is less important than the understanding of the nature of the dysregulation of the nervous system; however, the phenomenon (demonstrated in cats by Gellhorn, [@B43]) of extreme co-activation of sympathetic and parasympathetic systems under life-threatening conditions offers a compelling model for the freeze, collapse, and dissociation often observed in PTSD (Nijenhuis et al., [@B92]; Halvorsen, [@B57]); see Figure [5](#F5){ref-type="fig"}.

![**Traumatic stress response**. In the face of extreme challenge, when either the situation is extremely threatening and overwhelms the capacity of the organism to respond effectively, or if the response is prevented in some way (restraint), there is first an extreme sympathetic (ergotropic) activation with loss


dysfunction, the stimulus/response model has long been recognized as inadequate for explaining complex behavior. Control systems, such as the systems involved in autonomic regulation, require feedback and feed-forward loops which are not part of the explanatory framework of conditioning theory (Haken, [@B55]). Although we do not question the well-established knowledge concerning neuronal dendritic modification in response to conditioning, the behavior of complex neural networks are governed by higher-order principles of dynamical systems theory (Haken, [@B56]). Thus, in SE, symptoms are seen as due to a disorganized complex dynamical system, rather than resulting from a simple conditioning process (Levine, [@B74]). Fear conditioning extinction is the canonical model for recovery from PTSD, especially through exposure therapy (Rothbaum and Schwartz, [@B115]); however conditioning theory states that, in the extinction process, aconditioned fear response is not actually eradicated but only suppressed by competing (positive) conditioned experiences (McNally, [@B87]); the implication of this, born out by experience, is that, although fear de-conditioning is quick and effective, it is also easily disrupted, as re-exposure to trauma-related cues easily reinstate the fear response (Vervliet et al., [@B139]). By contrast, clinical experience in SE demonstrates a very robust change in fear responses which are remarkably resistant to re-evocation; this is consistent with the theory that clinical changes mediated by the SE process are not primarily due to fear conditioning extinction but to a discontinuous alteration in CRN dynamical functioning; in terms of dynamical systems theory, a shift to a different attractor basin (Abraham et al., [@B2], [@B1]).

Simon\'s inability to have volitional control over


that the sound of the traffic doesn\'t bother me right now!

### Discussion

The second half of the first session demonstrates the core of the methodology of SE. The first important concept is that of "*discharge.*" The sympathetic nervous system mobilizes the body for intense kinetic activity ("fight or flight"). Under normal circumstances this "biological energy" (the secretion of various neuroendocrine substances and activation of certain neural pathways) is used to power intense muscular activity; when successful, this arousal is part of a cycle involving mobilization, successful action, exhilaration, relaxation, and a return of the nervous system to baseline functioning. However, under certain conditions the ANS may get "stuck" in a state of excess activation; the muscular activity does not happen or is not successful, the reciprocal activation of theI was 7 I fell off my bike, I couldn\'t breathe. My dad got mad and made me get back on the bike and told me he was proud I didn\'t cry. I so much wanted to please him, even though I was just a little kid. (Tears start to flow freely down Simon\'s cheeks as he sobs gently.) I was so scared, so scared.... I think he was scared too; my dad. I think I never really cried after that, not till just now.

Me:

You\'re doing great, let the shaking and tears happen, just feel it... they\'ve been there for such a long time....

(Things settle over a few minutes. Then I notice Simon\'s body starts to gently jerk in the chair.)

Me:

What happens now?

Simon:

I\'m losing control! It\'s spinning! The


of three domains: an NH~2~-terminal protein 4.1, ezrin, radixin, and moesin homology (FERM) domain, a central kinase domain, and a COOH-terminal focal adhesion--targeting domain ([@B5]). The FERM domain associates with integrin molecules while the tyrosine residue 397 of the focal adhesion--targeting domain autophosphorylates upon stimulation by integrins. Activated FAK can subsequently phosphorylate other FAK tyrosine residues and associated Src homology 2 domain--containing proteins, such as Src and the p85 subunit of phosphatidylinositol 3-kinase (PI3K) ([@B6]).

In vivo small interfering RNA (siRNA) knockdown of FAK causes insulin resistance in liver and muscle by attenuating phosphorylation of insulin receptor substrate 1 (IRS1) ([@B7]). Similarly, antisense FAK in a cultured muscle cell line prevented actin reorganization, resulting in decreased GLUT4 translocation and downregulation of insulin/Akt (also known as protein kinase B) signalingdetection, cells were washed with PBS and fixed in 2% paraformaldehyde. Cells were coimmunostained using antiphospho-paxillin and either anti--synaptosomal-associated protein 25 (anti-SNAP25) or anti--syntaxin 1 (Sigma-Aldrich). Images were captured and colocalization coefficient analyses were performed using a laser scanning confocal imaging system (LSM510) equipped with LSM software (Carl Zeiss, Oberkochen, Germany).

Electrophysiology. {#s10}
------------------

Standard, whole-cell technique with sine+DC lockin function of an EPC10 amplifier and Patchmaster software (HEKA Electronics, Lambrecht/Pfalz, Germany) were used with experiments performed at 32--35°C as previously described ([@B17]). β-Cells were identified by size and the presence of a voltage-gated Na^+^ current that inactivated at approximately --90 mV (except when tetrodotoxin is present).

Electron microscopy. {#s11}
--------------------

Pancreatic islets were isolated from 4--8-week-old mice by collagenase digestion and cultured in RPMI 1640 medium without glucose for 1 h. Islets were


blood glucose levels, measured in 4--8- and 12--18-week-old mice, showed an increase in RIPcre^+^*fak*^fl/fl^ compared with their RIPcre^+^*fak*^+/+^ littermates in both age-groups ([Fig. 1*C*](#F1){ref-type="fig"}). We then performed GTTs, which demonstrated similar fasting blood glucose levels but impaired glucose tolerance in RIPcre^+^*fak*^fl/fl^ mice compared with RIPcre^+^*fak*^+/+^ controls in both age-groups ([Fig. 1*D*](#F1){ref-type="fig"}). To investigate whether the impaired glucose tolerance was due to changes in peripheral insulin sensitivity, ITTs were performed. The results showed similar blood glucose lowering after insulin injection between RIPcre^+^*fak*^+/+^ and RIPcre^+^*fak*^fl/fl^ mice in both age-groups ([Fig. 1*E*](#F1){ref-type="fig"}), which suggests that the impaired glucose tolerance in RIPcre^+^*fak*^fl/fl^ mice was not due to changes in peripheral insulin sensitivity but rather due to defects in β-cell mass and/or function. These results show that FAK in β-cells plays an essential\< 0.01; \*\**\*P* \< 0.001. Results represent mean ± SE. +,+/+, RIPcre^+^*fak*^+/+^; +,−/−, RIPcre^+^*fak*^fl/fl^. wks, weeks. (A high-quality digital representation of this figure is available in the online issue.)](1708fig3){#F3}

Impaired glucose-stimulated insulin secretion with normal GLUT2 and Ca^2+^ response in FAK-deficient β-cells. {#s17}
-------------------------------------------------------------------------------------------------------------

Insufficiency in either β-cell mass and/or function can lead to impaired glucose tolerance. Thus, we next examined whether FAK is also essential for β-cell function. Random plasma insulin level was decreased in RIPcre^+^*fak*^fl/fl^ mice ([Fig. 4*A*](#F4){ref-type="fig"}) but similar insulin content was maintained within similarly sized islets ([Fig. 4*B*](#F4){ref-type="fig"}). To further investigate β-cell function, we measured glucose-stimulated insulin secretion and found reduced insulin secretion in response to glucose in vitro ([Fig. 4*C*](#F4){ref-type="fig"}). Phase I and phase II insulin secretion were also suppressed in vivo in RIPcre^+^*fak*^fl/fl^ mice ([Fig.


mouse embryogenesis ([@B34]). Cell cycle--regulating proteins p27 and cyclin D1 were shown to be required for FAK-dependent cell cycle progression in glioblastoma cells ([@B24]). FAK has also been identified as a critical mediator of insulin signaling ([@B7]--[@B9]), participating in IR phosphorylation ([@B35]) and activating PI3K/Akt ([@B36],[@B37]) through phosphorylating the p85 subunit of PI3K, suggesting that FAK might associate with IRS/PI3K to activate insulin signaling and deletion of FAK in β-cells may directly attenuate insulin/Akt signaling, as shown in this study.

Genome-wide association studies have identified CDK5 regulatory subunit--associated protein-like 1 (CDKAL1) as one of the genes associated with the development of type 2 diabetes ([@B38]). A recent report indicates that siRNA knockdown of CDK5 expression in a rat insulinoma cell line (INS 832/13) leads to enhanced apoptosis through decreasedThe present invention relates to an automatic analyzer for clinical analysis and more particularly, is directed to an automatic analyzer which performs its own calibration in accordance with a standardized protocol used for correction of differences in measured data between the automatic analyzers of different facilities.
In clinical analysis fields, in recent years, it has been demanded to correct differences in measured data between automatic analyzers of different facilities, that is, standardize measured data. To this end, different standardized protocols are prescribed for different objects to be measured such as electrolyte, cholesterol and enzyme number.
Explanation will be made, in particular, in connection with the enzyme number. First, differences in measured data between the automatic analyzers result partly from the accuracies of the individual automatic analyzers. For the purpose of


and one-on-one counseling sessions. She became friends with the man who stared at her that first night, Jerry, with whom she enjoyed doing crafts.

“The first weeks were the easiest because my family was able to visit all day,” Peck said. “My mom spent every night with me. The support from my friends and family meant the world.”

But her second week in the mental health unit brought restlessness.

“I remember being angry and bitter,” Peck said. “I was sick of the blank white walls, the brown outfits, the lack of privacy — just everything.”

The hospital’s strict visiting hours allowed Peck to see her family only a couple hours each night.

“By the end of that second week, I wanted to be home so bad that I begged my mom to letPresident Obama will renew his appeal for world leaders to accept more refugees in his final appearance at the United Nations on Tuesday, even as a series of suspected Islamist terrorist attacks in the U.S. raised fresh questions about the administration’s plan for welcoming immigrants from Syria.

The president will convene a summit at U.N. headquarters in New York City, seeking agreements from countries to double the number of refugees resettled each year. To show his commitment, Mr. Obama plans to raise the U.S. limit on refugees next year to 110,000, up from 85,000.

As he prepared to make his international appeal, however, Mr. Obama faced renewed criticism at home from the campaign of Republican presidential nominee Donald Trump over the administration’s immigration and national security policies after suspected terrorist


and Seaside Park as acts of terrorism, he said the Islamic State group “is instigating a lot of people over the internet to carry out attacks.”

“They are trying to hurt innocent people, but they are also trying to instill fear in all of us,” Mr. Obama said. “We all have a role to play as citizens in making sure we don’t succumb to that fear.”

The administration also came under fire Monday for a comment by White House press secretary Josh Earnest, who said the U.S. is in a “narrative fight” with the Islamic State. He cautioned against portraying the fight as a battle between Islam and the West, saying that narrative helps extremists.

Mr. Trump rejected that argument.

“They are wrong to say that we’re in a fight about ‘narratives,’”package de.lessvoid.nifty.slick2d.loaders;

/**
 * This interface is the general interface for any resource loader that is defined for this Nifty-Slick renderer.
 *
 * @author Martin Karing <nitram@illarion.org>
 */
public interface SlickLoader {
}


know if any clarification is needed.

A:

This prevents having to read each file many times. It reads each file just once.
awk 'NR == FNR {a[$1]=1;next} $5 in a {$1="XYZ"} {print}' word.list.file old.file > new.file && mv new.file old.file

Explanation:
# if the current record number is the same as the record number in the file
# which means "if we're reading the first file"
NR == FNR {
    a[$1]=1  # put a flag in an array indexed by the contents of the first field
    next     # read the next line in the file and continue at the top of the script
}

# Now we're processing the second file
# if field 5 exists as an index in the array named "a" (it's a wordespecially lame since Weinstein has been a force behind many of Tarantino’s films and even threw the director an engagement party just a few weeks ago. Actress Lupita Nyong’o wrote a Times op-ed to tell of her harassment by Weinstein. And the #MeToo hashtag continues to go strong as women reveal their own stories of harassment.

Actress Evan Rachel Wood made an ominous statement on Twitter. She retweeted a story about Hollywood pedophilia with the comment: “This will be the next dam to break.”

Hurray For Hollywood: Paid cable has a reputation as the place where obscenity meets violence and gives birth to new programming. The latest example is the Showtime comedy “White Famous” that included 150 F-bombs in the first two episodes.

Actress “Hanoi” Jane Fonda is still at it,


to oppose the National Rifle Association on guns. It starred many actors who made their career in violent movies, including perennial anti-gun nut Julianne Moore. She’s currently featured in the latest “Kingsman” movie, which delivers enough gun violence to overthrow a small nation.

ABC’s “American Housewife” tried a new way to promote abortion – compare babies to ‘lice.” The episode titled “The Lice Storm” compared how one character had lice and another was three-months pregnant. “So, I also have a parasite sucking the nutrients out of my body,” one character said.Q:

If the core of a graph is a forest, then it is Class 1

It is a standard result, due to Fournier, that if the core of a graph (the induced graph by the vertices having their degrees equal to maximum degree of the graph) is a forest (acyclic), then the graph is Class 1 (edge colorable with colors equal to the maximum degree of the graph.
Is there a simple proof of this fact? Specifically, it is said that it follows from the Vizing Adjacency lemma. Any hints? Thanks beforehand.

A:

Thanks to the comment by @IlyaBogdanov, consider that the graph is not of Class 1, that is, its edge chromatic number is $\Delta+1$, where $\Delta$ be the maximum degree of the simple graph. Consider the minimal such graph, that


is, a critical graph. Now, this should consist of only two major vertices connected by an edge.
Now, we apply the Vizing adjacency lemma (VAL) to one of the major vertex. This implies, by VAL that the vertex should have at least two major neighbours. But, it is adjacent to exactly one major neighbour, which is a contradiction. Continuing the process by adding leaves of major vertices (as trees can be formed this way), we obtain the full theorem.

punishment if committed by the use of a deadly or
        dangerous weapon or device) for which he may be prosecuted in a court of the United

                                                  4
       In March 1996, Ruger pled guilty to the charges against him and agreed to testify for the

government at Mitchell's trial. At the trial, Hazel, Jackson, and Ruger all identified Mitchell as the

fourth participant in the robbery. They testified that


regularly called upon to assess whether the evidence adduced at trial could support any rational

determination of guilt beyond a reasonable doubt. Id. Moreover, this review is wholly distinct from

the jury's conclusion that the evidence on another count was insufficient. Consequently, as long as

the guilty verdict is supported by sufficient evidence, it must stand, even in the face of an

inconsistent verdict on another count. See id. at 67, 105 S.Ct. 471.

       In the case under review, the evidence amply supports the jury finding of guilty as to Count

One. All of the other participants in the bank robbery identified Mitchell as the fourth conspirator.

                    Bullards Bar Reservoir Fish Report

Bass can be found in a number of different areas. Some are chasing bluegill fry or shad near the top. Others are holding in deep water. Boat traffic is expected to pick up on the weekend due to dropping water levels in Folsom. Emerald Cove Resort & Marina (530) 692-3200


Q:

Coding multiple annotations swift 2

I have created code that produces a geotargeting area represented by an annotation and MKCircle.  The app notifies the user when they have entered and exited a region.  Everything is working fine but I cannot figure out how to get the app to hold/display multiple regions (only one annotation/circle will show) here are a few snippets of my code:
override func viewDidLoad() {
    super.viewDidLoad( )

//setup locationManager
locationManager.delegate = self
locationManager.distanceFilter = kCLLocationAccuracyNearestTenMeters
locationManager.desiredAccuracy = kCLLocationAccuracyBest
locationManager.requestAlwaysAuthorization()

//setup mapView
mapView.delegate = self
mapView.showsUserLocation = true
mapView.userTrackingMode = .Follow

//setup test data will need to link coredata to pass in (LocationLabel, radius, address)
    setupData("Test1", radius: 100, Address: "735 Main Rd, Clemson")
    setupData("Test2", radius: 100, Address: "821 Main Rd, Clemson")
    setupData("Test3", radius: 100,A comprehensive approach to the determination of two benzimidazoles in environmental samples.
Among the various pharmaceuticals regarded as emerging pollutants, benzimidazoles--represented by flubendazole and fenbendazole--are of particular concern because of their large-scale use in veterinary medicine and their health effects on aquatic organisms. For this reason, it is essential to have reliable analytical methods which can be used to simultaneously monitor their appearance in environmental matrices such as water, sediment and tissue samples. To date, however, such methods relating to these three matrices have not been available. In this paper we present a comprehensive approach to the determination of both drugs in the mentioned above matrices using liquid chromatography-ion trap mass spectrometry (LC-MS/MS). Special attention was paid to the sample preparation step. The optimal extraction methods were further validated


the Hungarian Zionist movement and their families. The Satmar rebbe, Rabbi Teitelbaum, did not at all fit in to our community, whose major leaders were intellectuals and academics who believed that as soon as liberation arrived, they would be greeted with great honor. Yet suddenly here they were so bitterly disappointed since here in Switzerland almost no one paid any attention to them. Everyone was interested in the personality known as the Satmar rebbe, Rabbi Yoel Teitelbaum. Everyone wanted to know where he was in the transport, and how he was feeling. Everyone asked the same question: Where is the rabbi?

So we all realized that it was not the professionals and academics who were indispensable, but rather the quiet and haggard holy man.

In Switzerland we all parted ways,but for a long time thereafter until today, I think a great deal about the fascinating personality of the Satmar rebbe. I frequently still remember the following little philosophical ideas:

When we consider the significance of religion, and the fact that the Torah and faith is what has preserved the Jewish People over thousands of years of Exile, we must also realize that the Satmar rebbe is without a doubt one of the holiest individuals produced by the Jewish People, and is the greatest guardian to assure that the Torah of Moses is not forgotten, G-d forbid. It may be possible that we perceive him as being too fastidious, too stubborn about following every point of the Torah, but without a doubt he is the real and most loyal


Heritability analysis of lipids and three gene loci in twins link the macrophage scavenger receptor to HDL cholesterol concentrations.
We studied 100 healthy monozygotic and 72 dizygotic twin pairs (mean age, 34 +/- 14 years) to test for genetic influences on blood lipids and to examine relevant gene loci. Total cholesterol (TC), LDL cholesterol (LDL-C), HDL cholesterol (HDL-C), and triglyceride (TG) levels were determined after a 12-hour fast. Zygosity was determined with the use of microsatellite markers. Heritability estimates were conducted by using the lisrel 8 program; a sib-pair analysis was conducted by using the sibpal program. Linear regression analyses were carried out between identical-by-descent status and squared within-pair differences of TC, LDL-C, HDL-C, and TG values. Heritability estimates of the lipid serum concentrations ranged from .58 to .66.of eCBs in this form of plasticity \[[@B119]\]. Further investigations have shown that this form of disinhibition is likely mediated by somatodendritic release of glutamate-filled vesicles expressing the vesicular glutamate transporter vGLUT3 with consequent activation of presynaptic metabotropic glutamate receptors (Figures [4(a)](#fig4){ref-type="fig"} and [4(b)](#fig4){ref-type="fig"}) \[[@B119], [@B118]\].

3.2. Spike Timing-Dependent Plasticity of GABAergic Synapses {#sec3.2}
------------------------------------------------------------

Spike timing-dependent plasticity (STDP) is a form of synaptic plasticity that requires both pre- and postsynaptic firing, inducing changes in synaptic strength whose polarity (potentiation or depression) depends on the temporal order of pre- and postsynaptic spiking. Glutamatergic STDP has been shown to follow precise general rules: long-term potentiation (LTP) of synaptic transmission is produced when presynaptic spiking precedes (in a millisecond time window) postsynaptic action potential, whereas LTD is induced when postsynaptic spikes precede presynaptic


several open questions remain, such as, for example: (i) what are the physiological activities (single neuron and/or network activities) necessary to induce plasticity of GABAergic synapses? (ii) Is there a heterogeneity or bidirectional plasticity of GABAergic synapses in different cortical areas? (iii) What is the functional role of GABAergic transmission during different cortical activities? (iv) What other neuromodulators, in addition to endocannabinoids and glutamate, can induce activity-dependent changes of GABAergic synaptic strength? (v) Could GABAergic plasticity lead to complex Cl^−^ gradients inside a principal neuron \[[@B124]\], such that the direction (inhibition versus excitation) of GABA-mediated responses might, in some cases, contribute to some forms of hyperexcitability? (vi) Is plasticity of inhibitory synapses altered in pathological situations? Addressing these questions will help define the fundamental molecular, cellular, and synapticmagic rather than as a science. First of all, astrologers have never attempted to explain how astrology supposedly works—that is, why the apparent positions of different astronomical bodies supposedly have different effects upon different people. As Bart Bok, former president of the American Astronomical Society, put it:

Many believers in astrology have suggested that each planet issues a different variety of special as-yet-undetected radiations or “vibrations” . . . [but] there is apparently conclusive evidence that the sun, moon, planets, and stars are all made of the same stuff, varieties and combinations of atomic particles and molecules, all governed by uniform laws of physics. It does not make sense to suppose that the various planets and the moon, all with rather similar physical properties, could manage to affect human


those forces are weak indeed. At its nearest conjunction, Mars exerts far less gravitational force upon a newborn infant than the midwife or doctor who delivers the child.

Fourth, astrologers, in their computations, do not take into account the inverse square law, which is a fundamental law of physics. It says that the amount of radiation received by a body varies as the inverse square of its distance from the source of the radiation. For example, the amount of light reaching a ship four miles from a lighthouse will be only one-quarter (per unit of surface area) of that reaching a ship two miles from the lighthouse, and one-sixteenth of that reaching a ship one mile from the lighthouse. But laws of physics do not matter to astrologers, andthe believers “remembered” that the article debunking ESP concluded that ESP exists.

Another of Gauquelin’s experiments provides a more amusing example of the self-deception of occult believers. He took out a newspaper advertisement in which he promised free personalized horoscopes to all who answered the ad. One hundred fifty persons responded. Gauquelin then sent out the same horoscope to all 150 and asked them how well it fit them. Ninety-four percent replied that they recognized themselves in it. The horoscope was that of Dr. Michel Petiot, a mass murderer.

Why do occult believers have such a reluctance to face facts? Glick and Snyder concluded that, “in order to maintain the sense of being able to predict events, the believer makes the facts ‘fit’ the theory whether or not these events


a bit recessed in the design and since my portable tripod uses an attachment plate, it almost didn’t have enough clearance to allow it to securely attach. After all, I didn’t want to drop OSMO! I was able to get it attached but would have felt better if I could have gotten another turn out of that screw!

Getting back to what I was saying about not knowing what it taste like, well, I was surprised that the resolution of the created video was only 1280 x 720. While the OSMO was set to 4K, I was surprised the end result of this test only yielded an HD output. Please note, the video posted below was captured in OSMO’s Time Lapse Video mode and not the photo Interval mode.1.. Introduction
================

Turkey is one of the most seismically active regions on the earth. Different fault systems in Anatolia and the surrounding regions were created due to the complex plate interactions among Arabia, Eurasia and Africa \[[@b1-sensors-09-01471]\]. The North Anatolian Fault Zone (NAFZ) and the East Anatolian Fault Zone (EAFS) are the main strike-slip fault belts in Turkey where several earthquakes have occurred, resulting in huge numbers of fatalities over the past several hundred years ([Table 1](#t1-sensors-09-01471){ref-type="table"}).

Earthquakes and other natural hazards can cause disasters of uncontrollable magnitude when they hit large urban areas. Emergency response and early recovery assessment in earthquakes require rapid and reliable damage assessment and loss estimation. In the case of suddenly occuring earthquakes, remote sensing data can be reliably used to create fast draft damage


figure is also a summary of the methodology section.

4.. Results
===========

The original pre- and post-earthquake images are shown in [Figures 4 a and b](#f4-sensors-09-01471){ref-type="fig"} and the FFT images generated from these data are illustrated in [Figures 4 c and d](#f4-sensors-09-01471){ref-type="fig"}. As a result of collapsed buildings and roads, spatial structure and texture of the post-earthquake image had changed. This caused differences in spatial frequency which can be determined via Fourier Transform. The differences in spatial frequency for pre- and post-earthquake data can be identified from [Figures 4 c and d](#f4-sensors-09-01471){ref-type="fig"}.

The low frequencies are plotted near the origin (center) while the higher frequencies are plotted further out. Generally, the majority of the information in an image is in the low frequencies indicated by the bright areas at the center ofthe [Figures 4 c and d](#f4-sensors-09-01471){ref-type="fig"}.

High pass filter was applied to satellite images in frequency domain to delineate the border of linear objects like roads and buildings precisely. Filters were applied to the low frequencies which are around the center for both pre- and post-earthquake data. After the filtering, IFFT was applied and edited Fourier images were converted back into the spatial domain.

After the visual interpretation of IFFT images, a difference image was generated by subtracting post-IFFT-image and pre-IFFT-image. Level slicing method with 10 slices was conducted to identify the damaged and non-damaged areas. Different numbers of levels were tried to find out the optimum number of slices and the analyses shows that having a slice number higher than 10 did not contribute significant information since only a


hard to identify with current spatial resolution. Most of the parcels which have plenty of collapsed buildings were easily identified with 75 % or higher accuracy.

Total accuracy of the proposed method was calculated by rationing the sum of diagonals of all blocks to total number of parcels. The total accuracy obtained from this ratio is 80.19 %. The results illustrated that the proposed method can be successfully used to identify earthquake-induced damage areas automatically. Considering the spatial resolution of the satellite image used in this study, this accuracy value is reasonable. The results derived from this research can provide important information to many decision-makers and local authorities to determine location and magnitude of destructions and conduct emergency operations. However, depending on the end-user needs, if higher accuracy valueX-1 has a luminosity ($\sim 10^{40}$ erg s$^{-1}$), higher than that of X-2 ($\sim 5 \cdot 10^{39}$ erg s$^{-1}$). Variability of a factor $\sim 3$ and $\sim 5$ is observed for X-1 and X-2, respectively.

We further investigated the behaviour of the soft component in both sources. X-1 shows the disc component only in 5 observations while X-2 in 9 observations. In X-1 the luminosity of the soft component is $\sim 10^{39}$ erg s$^{-1}$ and it represents a significant part of the total flux. There is essentially no correlation between the luminosity of the disc component and the inner disc temperature. The same holds true also for the total luminosity. On the other hand, for X-2 the luminosity of the soft component is only a small fraction of the