which are conventionally used to house the radiation lamps, are rated at an ultraviolet transmittance (UVT) of 80 to 90% when brand new. Maintaining the % UVT at or very close to 80% is highly desirable to sustain the ability to meet disinfection requirements.
Fouling on an ultraviolet radiation surface (e.g., the quartz sleeve surrounding the lamp) is complex and can vary from site to site. The three main contributors to fouling include inorganic deposits, organic fouling and biofilms (which can grow when the surfaces are fouled and not fully irradiated)—see Kreft.
The major fouling components of inorganic scale deposits typically comprise one or more of magnesium hydroxide, iron hydroxide, calcium hydroxides, magnesium carbonate, calcium carbonate, magnesium phosphate and calcium phosphate. These are salts with inverse solubility characteristics—i.e., the solubilitydeep down in its gut where John Cena lives. Say what you will about Occupy, but you can’t deny that while it was going on, the nation’s attention was on Wall Street.

During the 2011/ 2012 Occupy movement, for the first time in years the entire country took a critical eye to the institution that was running our economy (by “running” I mean “dry humping”). The nation FINALLY cared that this institution was steadily extracting all of the wealth and resources and giving it (in an unmarked bag) to a tiny percentage of men and women (mainly men) who smelled impeccable. Even those citizens who were wrongly disgusted by Occupy still felt that Wall Street was exploiting the American people at a jaw-dropping pace. And they got angry. The


be this visually stimulating.

Oh, Pinterest. It's safe to say I'm bordering on obsession. No. No bordering... teetering on the edge. No... most of my body has crossed the line, but I'm keeping a tiny little pinky grasping the edge just so I don't seem completely crazy...

*Be sure to check the right sidebar for all the fun parties I link to!

All content including images is protected under a creative commons copyright agreement. Please do not use my original content without previous consent, especially pictures of my kids. When applicable, I credit all of my “borrowed” ideas to the responsible party. (and you should too!) Thanks!with our friends at Site Organic to dream up a way to feed content from the central campus site out to the individual campus sites. This way, I can create one page in our central site and make that page available to the other campuses for syndication. The content can only be edited in one place – the initial place where the content is created, making content management pretty easy. Other wise we would be creating content again and again for every campus page.

4. Where do you draw the line between giving a campus its own freedom and personality online, vs. maintaining one consistent “brand” across the entire organization?
This is a really interesting question. After launching our first campus we realized that we needed to define what was


impact on secular Washington, DC with the message of Jesus Christ and Pastor Lon has often referenced our multi-campus strategy as creating a “spiritual beltway” around the District of Columbia. This logo mark is intended to be symbolic of that mission with the shape of the District as the starting point.

The three separate sections of the logo mark point “out” and “up” from the center of the image symbolizing that our focus is not only on the actual District but also the entire DC-metropolitan area. Each piece of the logo mark is a different shape and color, symbolizing that our campuses are “fraternal” twins as opposed to “identical” twins, meaning that while the core MBC DNA for each campus is the same, each campus will have its ownthe voxel. The noise within the image will also be reduced as the image thickness increases due to an increase in the number of photons used to create the image. The increase in photons allows thicker slices to use a lower dose to achieve the same SNR. This relationship between slice thickness and image quality makes the SSP an important factor during CT protocol optimization. Furthermore, SSP measurement is a recommended part of CT acceptance testing, as indicated by the AAPM Report 39 and IEC 61223‐3‐5 reports.[^10^](#acm20281-bib-0010){ref-type="ref"}, [^11^](#acm20281-bib-0011){ref-type="ref"}

During the infancy of Helical CT, much research was done to determine what factors of the CT scanner affected the axial resolution.[^1^](#acm20281-bib-0001){ref-type="ref"}, [^2^](#acm20281-bib-0002){ref-type="ref"}, [^3^](#acm20281-bib-0003){ref-type="ref"}, [^4^](#acm20281-bib-0004){ref-type="ref"}, [^5^](#acm20281-bib-0005){ref-type="ref"}, [^6^](#acm20281-bib-0006){ref-type="ref"}, [^7^](#acm20281-bib-0007){ref-type="ref"}, [^8^](#acm20281-bib-0008){ref-type="ref"}, [^9^](#acm20281-bib-0009){ref-type="ref"} The size of the focal spot, the width of the detectors,


produce reliable results when assessing the slice width. However, it is critical that the SSP be generated from the maximum value in the ROI when possible, or a small ROI is used when only the mean value can be obtained.
Samarcand Manor State Industrial Training School for Girls

The Samarcand Manor State Industrial Training School for Girls was situated near Jackson Springs, twenty miles from Southern Pines and twelve miles from Star, North Carolina.

The idea for the institution originated with Mrs. A.A. McGeachy of Charlotte, and it was funded by the state in 1917. The first board of managers, consisting of A.A. McGeachy, president; Elizabeth Delia Dixon-Carroll, Mrs. J.R. Chamberlain, W.S. Blakeney, Mrs. Stephen C. Bragaw, and Mrs. Agnes McNaughton, the superintendent, held its first meeting on May 28, 1919.

The site was purchased from C.H. Henderson. Three buildings and a "chalet" were constructed. The first girl arrived on September 3, 1918. The next year there were 134 girls and women, with the average age being 19.

The infirmary at the


unfamiliar.
*1314 Regarding the voices which Dyer said he heard, the witness said that Dyer told him that they were of a threatening nature; told him to do "bad things"; were hard to understand; and called him a "M.F." Dyer told McCaughn that he knew the voices were not real and was not going to do anything about them. After questioning Dyer, McCaughn did not believe he would do anything.
McCaughn stated that he thought Dr. Erickson was present during the questioning about the voices.
The witness said that he did not have Dyer's complete records at the time and did not see a need to ask for them.

DR. MILES ERICKSON
Dr. Erickson is a psychiatrist who, on October 3, 1985, was employed as a staff psychiatrist and assigned to an openward at the Gulfport division.
Referring to his notes found on pages 917-921 of plaintiff's Exhibit 1(C), Dr. Erickson represented that he interviewed Dyer at "15:30." He noted that Dyer was diagnosed as a schizophrenic decompensating and that he was restless, hearing voices, talkative and cooperative.
Dr. Erickson stated that Dyer had already been assigned to an open ward by Biloxi staff and he countersigned the order.
Dr. Erickson was not the regular psychiatrist assigned to this ward and had never seen Dyer prior to October 3, 1985; however, he and Bill McCaughn worked together on Dyer's interview.
He could have placed Dyer in a closed ward; however, Dyer gave him no indication that he should change what Biloxi had started.
Responding to questions as to what minimum symptoms or behaviors would justify


putting a patient in a closed ward and holding him for 72 hours or five days, Dr. Erickson stated that by some means he would have to indicate that he would do harm to himself or someone else.
Dr. Erickson repeatedly asserted that the patient's past activities could not be the basis for detention.
Regarding the voices which Dyer was said to have heard, Dr. Erickson said that he did not hear Dyer say they were telling him to do bad things. It was the witness' opinion that voices are a secondary symptom of schizophrenia and are not "that important."
At the time of the interview Dr. Erickson did not have Dyer's prior records.

DR. J.W. SEASTRUNK
Dr. Seastrunk is a psychiatrist who, having heard the testimony and reviewed the exhibits, is offeredwanted him to come in. She saw no reason why Dyer should not be on her ward.
The witness stated that she saw Dyer about 5:00 p.m. when he went to the dining hall with three or four staff members and other patients and once again between 6:30 p.m. and 7:00 p.m. when he was going to Chapel for recreation and food.
At 9:00 p.m., when they did the bed check Dyer was missing.

DR. WOOD C. HIATT
Dr. Hiatt is a psychiatrist at the University of Mississippi Medical Center in Jackson, Mississippi, who is familiar with the VA Hospital at Gulfport and Jackson.
Dr. Hiatt testified that:
1. Dyer was properly evaluated and admitted;
2. Dyer was seen by several people over a period of several hours;
3. The assessments made were adequate; and
4. The


of costs for emergency room treatment, P-9, which totalled $117.90; and a receipt for replacement of bed linens, P-10, costing $160.56. She also stated that she was seen by her OBGYN and went to the Health Department for VD tests. All tests were negative.

DISCUSSION
Citing Grisham v. John Q. Long V.F.W. Post, No. 4057, Inc., 519 So.2d 413 (Miss. 1988), the defendant asserts that under Mississippi substantive law the elements of a cause for negligence are: (1) a duty owed by the defendant to the plaintiff; (2) a breach of that duty; (3) damages; and (4) a causal connection between the breach and the damages.
Both parties substantially agree that the central broad issue herein is what duty, if any, is owed to a third party by a psychiatric careand location of guards and guard stations during the time period involved.
The Court notes the aforementioned, not in an attempt to detract from the plaintiff's case as pled and argued, but because it appears that a bit more supervision might have prevented the elopement.
The testimony regarding control of voluntary patients assigned to open wards was generally that they had complete freedom to go outside onto the grounds and that, while they were urged to stay, they could leave if they chose; either by eloping or against medical advice.
Apparently, the principal reaction to elopement or to leaving AMA is to discharge the patient unless he or she indicates an inclination to leave while appearing to be harmful to themselves or others.
Referring to Miss.Code Ann. § 41-21-103(6) (1972) (as amended


from gene expression profiling in normal mucosal and HNSCC tumor samples. Bisulfite-treated DNA was amplified for the 5′ region that included at least a portion of the CpG island within 1--2 kb of the first exon of 126 genes, using primer sets (data not shown). The promoter regions of the genes were found from the database of the University of California, Santa Cruz, USA (UCSC) (<http://genome.ucsc.edu/>). Primer sequences were determined by the MethPrimer program ([@b16-ijo-42-04-1427]) showing the CpG islands in the promoter regions of 126 genes for bisulfite sequencing. The primers for bisulfite sequencing were designed to hybridize to regions in the promoter without CpG dinucleotides. PCR products were gel-purified using the QIAquick Gel Extraction kit (Qiagen) according to the manufacturer's instructions. Each amplified DNA sample was sequencedby inhibiting thioredoxin activity or by limiting its bioavailability and acts as a transcriptional repressor, between transcription factors and co-repressor complexes and its overexpression induces G0/G1 cell cycle arrest and is necessary for the maturation of natural killer cells ([@b22-ijo-42-04-1427]--[@b25-ijo-42-04-1427]).

Tumor suppressor candidate 2 is encoded by the *TUSC2* gene which is a highly conserved lung cancer candidate gene ([@b26-ijo-42-04-1427],[@b27-ijo-42-04-1427]). In malignant pleural mesothelioma ([@b28-ijo-42-04-1427]) and nasopharyngeal carcinoma ([@b29-ijo-42-04-1427]), expression of the *TUSC2* gene was found to be downregulated. *TUSC2* is often deleted in lung, breast, head and neck, renal and other types of cancer ([@b30-ijo-42-04-1427]) and was reported to be methylated in human lung cancer cells ([@b31-ijo-42-04-1427]). Furthermore, large-scale analysis of *TUSC2* expression in lung cancer and in bronchial squamous metaplastic and dysplastic lesions showed reduced expression levels


in saliva, serum or plasma samples from HNSCC patients.

This study was supported by the National Institute of Dental and Craniofacial Research (R37DE012588), The National Institute of Dental and Craniofacial Research/The National Cancer Institute (SPORE-P50DE019032) and Scientific Research Projects Coordination Unit of Istanbul University (UDP-22027). S. Dasgupta is supported by Elsa U Pardee Foundation. This report/analysis is based on a web database application provided by Research Information Technology Systems (RITS)-<https://www.rits.onc.jhmi.edu/>.

![The workflow of discovery approach via gene expression array technology.](IJO-42-04-1427-g00){#f1-ijo-42-04-1427}

![Expression array values from normalized microarray results of the *GNG7* gene: (A) from cell lines treated with 5-aza and non-treated with 5-aza; (B) human tumors and normal tissue samples.](IJO-42-04-1427-g01){#f2-ijo-42-04-1427}

![Expression array values from normalized microarray results of the *TXNIP* and *TUSC2* genes: (A) from cell lines treated with 5-aza and non-treated withlevel of dehydration you had coming in." Wondering if you've had enough water? Well, there's an app for that. Download iPee Daily to your iPhone to track your daily hydration status based on your weight and pee color. (We swear, we're not making this up!)

(Just about) everyone should eat more... omega-3-rich foods, especially fatty fish like salmon and rainbow trout. "We know that omega-3s are really good from an anti-inflammatory perspective, and for joint health too. It's one of those foods that athletes hardly ever eat as often as they should, and it can actually help them avoid injury and stay healthy."

(Just about) everyone should eat less... processed food. "Look at the degree of separation from the original food source. If it's really far away (or you can't


thought.

In the lives of
those living in the United States, this problem is compounded by
puritanism, which underscores the lockstep linearity of rationalism
with a very specific goal: eternal salvation. By focusing only on
those activities deemed holy, we both assure our places in heaven and
avoid the temptations of those things we do for fun, because fun
things lead us to inevitable perdition.

But neither science
nor puritanism are quite as powerful in our lives as the free market
business model. While it is true that organizations like The American
Enterprise Institute, backed by laissez-faire billionaires such as
Charles and David Koch, have done a lot to promote free market
thought in the last 40 years, the ideas go back much farther than
that. After all, Charles Dickens was already savagely critiquing the
point of view that every human activity mustPancreatic lymphoma, diagnosis and management.
Eleven cases of non-Hodgkin's lymphoma were referred as patients thought to have pancreatic cancer. It was impossible to make the diagnosis in this particular group of patients preoperatively, by imaging techniques and needle biopsy. For this reason we favor laparotomy for staging followed by Roux-en-Y choledochojejunostomy for those patients with obstructive jaundice because this approach both rapidly resolves the jaundice and enables a more accurate diagnosis. We do not feel that non-surgical means of decompression of the biliary tree by transhepatic or endoscopically placed stents are as safe or effective for patients who are potentially curable because the diagnosis may not be made early enough. We call this group of patients to your attention because recent reports indicate that over 30% of such patients


U.S. Code.

Links to other Internet based Tax Research materials.

Fill-in, print, and save features for most tax forms.

Internal Revenue Bulletins.

Toll-free and email technical support.

Two releases during the year.– The first release will ship the beginning of January
2012.– The final release will ship the beginning of March
2012.

Purchase the DVD from National Technical Information Service (NTIS) at
www.irs.gov/cdorders
for $30 (no handling fee) or call 1-877-233-6767 toll free to buy the DVD for
$30 (plus a $6 handling fee).(!GetChecked())
    SetChecked(true);
  LabelButton::NotifyClick(event);
}

ui::NativeTheme::Part RadioButton::GetThemePart() const {
  return ui::NativeTheme::kRadio;
}

void RadioButton::SetChecked(bool checked) {
  if (checked == RadioButton::GetChecked())
    return;
  if (checked) {
    // We can't start from the root view because other parts of the UI might use
    // radio buttons with the same group. This can happen when re-using the same
    // component or even if views want to use the group for a different purpose.
    Views other;
    GetViewsInGroupFromParent(GetGroup(), &other);
    for (auto* peer : other) {
      if (peer != this) {
        DCHECK(!strcmp(peer->GetClassName(), kViewClassName))


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Augusta, Kentucky

While Augusta has its share of antique shops and pubs, as well as top-notch restaurants that draw gourmands from all over the Bluegrass State, the most pleasurable thing to do may be to sit on a Riverside Drive bench and watch the Jenny Ann ferry glide back and forth across the Ohio River, as it has since 1798. Aside from pretty vistas, this city of 1,300 is known as the former home of "Mambo Italiano" singer Rosemary Clooney—whose house has been converted into amuseum—and her dashing nephew, George, who attended Augusta Independent High School. "George's parents still live here," says Doug Padgett, Augusta's director of tourism, "but when he comes back into town, he's pretty discreet about it."

The HousesRiverside Drive is lined with stately 19th-century brick rowhouses built for the old town's river merchants, who transported tobacco, livestock, and wine. Today, several small wineries call this part of Kentucky home. Both townhouses and detached houses here run from $250,000 to $400,000—the view makes them worth every penny—but more affordable houses can be found along Second and Fourth streets, where we spotted a completely renovated four-bedroom Queen Anne for $185,000.

Why Buy Here?"This town hasn't changed for a long time," says Padgett. "You can still see the remnants of the good old days—the


compatible reports. The concept of classification of the websites with corresponding group classification of ads to be run is used to edit ad availability from an extraordinarily large number of available ads to a manageable play lists which can be sorted at the browser. Finally, a system of ordering the play of ads in the overall process of playing many ads against many targeted numbers of plays is disclosed enabling total ad play to be centrally controlled with minimal computational burden on the advertising system central controller.we describe a retrospective patient series, essentially 17 individual case reports, of advanced cancer patients treated with oncolytic adenoviruses in the context of an Advanced Therapy Access Program (ATAP) who underwent radiological response evaluation with both contrast-enhanced CT and FDG-PET. Third, we collected a retrospective case series of radiological response and survival data of 182 patients treated with oncolytic adenoviruses in ATAP to evaluate the prognostic reliability of CT and FDG-PET. Overall, responses in CT and FDG-PET correlated well with each other and were equally reliable as prognostic markers for long survival after oncolytic adenovirus treatment. Interestingly, we observed that new FDG-avid lymph nodes appearing in FDG-PET after virus treatments may represent inflammatory responses and therefore should not be interpreted as treatment failure in the absence of other


they would be equally authorised by taxes by the States on exports. 4. The Southn. States being most in danger and most needing naval protection, could the less complain if the burden should be somewhat heaviest on them. 5. we are not providing for the present moment only, and time will equalize the situation of the States in this matter. He was for these reasons agst. the motion

Mr. WILLIAMSON considered the clause proposed agst. taxes on exports as reasonable and necessary.

Mr. ELSEWORTH was agst. Taxing exports; but thought the prohibition stood in the most proper place, and was agst. deranging the order reported by the Committee

Mr. WILSON was decidedly agst. prohibiting general taxes on exports. He dwelt on the injustice and impolicy of leaving N. Jersey Connecticut &c    \<0.001      0.027        \<0.001

The oxygen saturation of different refractive groups. The classification criteria are defined as follows: hyperopia: SE ≥ +0.5 D; emmetropia: −0.5 D \< SE \< +0.5 D; Myopia: SE ≤ −0.5 D. mild myopia: −3.0 D \< SE ≤ −0.5; moderate myopia: −5.0 D \< SE ≤ −3.0; and high myopia: SE ≤ −5.0. *F*:*F* value in one‐way [anova]{.smallcaps}. p: p value in one‐way [anova]{.smallcaps}.

John Wiley & Sons, Ltd

Independent factors associated with retinal oxygen saturation {#aos14043-sec-0018}
-------------------------------------------------------------

Age, gender, BMI, IOP and AL were included in a multivariate analysis of SaO~2~, SvO~2~ and AVD using multiple linear regression (Table [4](#aos14043-tbl-0004){ref-type="table"}). Since SE was strongly collinear with AL, it was not included in the establishment of the model, considering the fact that SE can relate to a number of parameters other than


- -3 - o)*2. Calculate the greatest common factor of 18 and s.
18
Let d be (-11)/(-1) - (-26 + 24). Calculate the greatest common divisor of 520 and d.
13
Let h(j) = 5*j**3 - 4*j - 4. Let u be h(-2). Let f be u/5*(-30)/9. What is the greatest common factor of 16 and f?
8
Let b be 308/21*(1 - -2). Suppose -3*q + q + b = 0. Let i(m) = m**3 - 7*m**2 + 3*m + 1. Let v be i(7). What is the greatest common divisor of v and q?
22
Suppose 0*g - 3*g = -27. Let u be -5 + 241 + 0 + (6 - -1). Calculate the greatest common factor of u and g.
9
Suppose 3*w = -w - 20, -2*l + 3*w = -279. Letf(d) = 3*d**2 + 2*d - 5. Let q be f(2). Calculate the highest common divisor of l and q.
11
Let v(a) = -8*a**3 - a**2 - 16*a - 60. Let s be v(-4). What is the highest common factor of s and 40?
20
Suppose -u = 5*i, -3*u + 65 - 5 = -5*i. Let g = 109 - 109. Suppose 6*m - m - u = g. What is the greatest common divisor of m and 6?
3
Suppose 6*c + c - 165 = 2*n, 2*n - 135 = -5*c. Suppose u = -u - r + 247, 4*r + 387 = 3*u. What is the highest common divisor of u and c?
25
Suppose -5*g - 4*w + 20 = 0, 3*g - g + w = 11. Let u(o)


     <attribution license="cc4" from="Microsoft" modified="false" />
      <para>You can get attributes for files and directories by calling the <see cref="M:System.IO.File.GetAttributes(System.String)" /> method, and you can set them by calling the <see cref="M:System.IO.File.SetAttributes(System.String,System.IO.FileAttributes)" /> method.</para>
      <para>It is not possible to change the compression status of a <see cref="T:System.IO.File" /> object by using the <see cref="M:System.IO.File.SetAttributes(System.String,System.IO.FileAttributes)" /> method. Instead, you must actually compress the file using either a compression tool or one of the classes in the <see cref="N:System.IO.Compression" /> namespace.</para>
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    <summary>
      <attribution license="cc4" from="Microsoft" modified="false" />
      <para>Provides attributes for files and directories.</para>
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        <para>The file is hidden, and thus is not included in an ordinary directory listing.</para>
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    <Member MemberName="IntegrityStream">
      <MemberSignature Language="C#" Value="IntegrityStream" />
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is offline. The data of the file is not immediately available.</para>
        </summary>
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      <ParametersMovie tit scenes

Housewife made to be lesbian slave

But Hole's investigation is complicated by his relationship with his ex-girlfriend Charlotte Gainsbourg and being a father figure to her son Michael Yates.

When you first see the sculpture, do you see yourself in it? Movie review by Jeffrey M. Brandin Rackley - Life on top first date. Sexy girls getting fuked. Ana was submissive, naive, and innocent in the first film, but in this installment, she took more caution and control over her relationship with Grey. Movie tit scenes. KaralexisU. Common Sense Media's unbiased ratings are created by expert reviewers and aren't influenced by the product's creators or by any of our funders, affiliates, or partners.

Emily Middleton Amy Schumer - a self-involved, bawdy-mouthed, irresponsible young millennial female addicted to Instagramrecently fired


I cancelled it. Never let someone hold you up!"

The military estimated Trump's military parade would cost taxpayers $92 million, an increase of $80 million above the previously estimated cost.

The new numbers come from figures worked up by the Department of Defense, as well as interagency partners that would be needed for the parade to go off.

"I will instead attend the big parade already scheduled at Andrews Air Force Base on a different date, & go to the Paris parade, celebrating the end of the War, on November 11th," Trump added.

Nov. 11 is Veterans Day.

Trump has been obsessed with the idea of a military parade since he saw the Bastille Day parade in France last year.

He has ignored naysayers who complained about the cost of the parade, even beforedismissed Mendias-Mendoza’s appeal, agreeing that he had pro-
vided insufficient evidence that he was an LPR at the time of his 1991 depor-
tation proceedings. The BIA also concluded that Mendias-Mendoza had failed
to give material evidence that was previously unavailable, to submit an appli-
cation for relief, or to establish prima facie eligibility for the relief sought. The
BIA similarly declined to exercise its sua sponte authority to reopen.

                                         II.
      “Motions to reopen deportation proceedings are disfavored,” and the
party seeking relief has a heavy burden.


Mental disorders in children and adolescents with sickle cell disease.
This study compares the rate of mental disorders in children and adolescents with sickle cell disease with the rate in a corresponding control population. Using the clinical interview, a diagnosis based on DSM-III-R criteria for the presence of 13 mental disorders common to childhood was considered in a sequential sample of 39 subjects with sickle cell disease and a convenience sample of 26 same-race control subjects. Thirty-one percent of the sickle cell group and 42% of the control group screened positive for one or more of the selected mental disorders. The difference was not significant. When subgroups of mental disorders were considered (anxiety disorders, depressive disorders, disruptive behavior disorders, psychotic disorders, and other selected disorders), the differences were stillfor your AWS Elemental MediaConnect account."
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      "documentation": "The output that you want to add to this flow.",
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          "shape": "__listOfEntitlement",
          "locationName": "entitlements",
          "documentation": "The entitlements in this flow."
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          "shape": "__string",
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      "type": "structure",
      "members": {
        "Description": {
          "shape": "__string",
          "locationName": "description",
          "documentation": "A description of the output."


 },
        "EntitlementArn": {
          "shape": "__string",
          "locationName": "entitlementArn",
          "documentation": "The ARN of the entitlement on the originator''s flow. This value is relevant only on entitled flows."
        },
        "MediaLiveInputArn": {
          "shape": "__string",
          "locationName": "mediaLiveInputArn",
          "documentation": "The input ARN of the AWS Elemental MediaLive channel. This parameter is relevant only for outputs that were added}
      },
      "required": [
        "FlowArn",
        "OutputArn"
      ]
    },
    "RemoveFlowOutputResponse": {
      "type": "structure",
      "members": {
        "FlowArn": {
          "shape": "__string",
          "locationName": "flowArn",
          "documentation": "The ARN of the flow that is associated with the output you removed."
        },


      "documentation": "The name of the source."
        },
        "SourceArn": {
          "shape": "__string",
          "locationName": "sourceArn",
          "documentation": "The ARN of the source."
        },
        "Transport": {
          "shape": "Transport",
          "locationName": "transport",
          "documentation": "Attributes related to the transport stream that are used in the source."
        "Url": {
          "shape": "__string",
          "locationName": "url",
          "documentation": "The URL from the API Gateway proxy that you set up to talk to your key server. This parameter is required for SPEKE encryption and is not valid for static key encryption."
        }
      },
      "documentation": "Information about the encryption of the flow."
    },
    "UpdateFlowEntitlementRequest": {
      "type": "structure",
      "members": {


"The flow that is associated with the entitlement that you want to update."
        },
        "Subscribers": {
          "shape": "__listOf__string",
          "locationName": "subscribers",
          "documentation": "The AWS account IDs that you want to share your content with. The receiving accounts (subscribers) will be allowed to create their own flow using your content as the source."
        }
      },
      "documentation": "The entitlement fields that you want to update.",
      "required": [
  "FlowArn": {
          "shape": "__string",
          "locationName": "flowArn",
          "documentation": "The ARN of the flow that is associated with the updated output."
        },
        "Output": {
          "shape": "Output",
          "locationName": "output"
        }
      }
    },
    "UpdateFlowSourceRequest": {
      "type": "structure",
      "members": {


                                NOT FOR PUBLICATION WITHOUT THE
                               APPROVAL OF THE APPELLATE DIVISION
        This opinion shall not "constitute precedent or be binding upon any court." Although it is posted on the
     internet, this opinion is binding only on the parties in the case and its use in other cases is limited. R. 1:36-3.

     timely report

in accordance with N.J.A.C. 12:17-4.2(b).

        Because Keeler did attempt, within time, to submit her claim through the

methods prescribed by the Department of Labor's Division of Unemployment

Insurance (Division), and it was unsuccessful not because of her oversight,

neglect, or misunderstanding of the law, but rather because of technical

difficulties that were not within her control, we reverse and remand for a

determination of benefits for the week of December 23 through December 29,

2018.

        Keeler has been employed by Maschio's food service, a school lunch

program, as a cook since 2005, where she works Monday through Friday from

7:30 a.m. until 2:00 p.m. She is paid around $16 per hour. Every year, her

employer closes for the Christmas holiday, which in


                      4
              thinking I did something wrong or there was a glitch in
              the computer. . . .

Keeler also testified she did not receive an error message, e-mail, or anything

else to indicate her December 26 claim did not go through. It was not until

January 4, when her husband's $100 benefits claim was deposited into their

account, but nothing additional for her claim, that Keeler realized something

was wrong.

      Keeler then testified she tried to file her claim again                                                 A-3382-18T3
                                        6
getting a post instructing her to try later. She contends that when she finally got

through on the phone, she was put on hold for over twenty minutes, after which

she was disconnected.

      Keeler argues that


The invention relates generally to over voltage protection circuits for protecting other circuits from higher than desired voltage levels, and more particularly to electrostatic discharge (ESD) protection circuits for protecting an input to a protected circuit.
There is a constant challenge to continuously design smaller, faster and more complicated integrated circuits to provide increased functionality for multimedia applications and other applications. With the continued demand for higher speed and lower power consumption integrated circuits a need exists for simple, low cost and reliable over voltage protection circuits. Graphics controller chips, like many integrated circuit devices, utilize CMOS, logic cores, and associated I/O pads as part of their circuit makeup. I/O pads can include, for example, input/output buffers coupled to a common pad or pin. For example, CMOS based videographics chips with 128 input/output ports (I/O ports) are required to operate at clock speeds of 125 MHz to 250 MHz or higher. Such devices may use a 2.5 V or 1.8 V power supplies for much of their logic to reduce power consumption. One way to increase the operating speed of such devices is to decrease the gate length of core circuitry transistors. However, a decrease in the gate length and gate oxide thickness of MOS devices can reduce safe operating voltage to lower levels.
For example, where an integrated circuit contains digital circuitry that operates from a 1.8 V source and is fabricated using silicon dioxide gate thickness of 30 Angstroms, a resulting safe operating voltage may be approximately 2.8 V. Such IC""s must often connect with


to have a circuit that is relatively inexpensive that utilizes a single gate oxide design to reduce costs while also allowing suitable protection against electrostatic discharge damage. Moreover, it would be advantageous if the electrostatic protection circuitry were effectively disabled during normal operations so that current is not drawn unnecessarily.
Consequently there exists a need for an ESD protection circuit that can be used for low supply voltage circuits while also withstanding higher input signal voltages. It would desirable if the protection circuit were a single gate oxide circuit.Cycling at the 2018 Summer Youth Olympics

Cycling at the 2018 Summer Youth Olympics was held from 7 to 17 October. The events took place at the Parque Tres de Febrero in Buenos Aires, Argentina.

Format

For the Youth Olympics instead of competitors competing separately they compete as a team. In the boys' and girls' combined team event two athletes compete together. The event contains five stages; three road events (road race, criterium and time trial) and two cross-country events (cross-country eliminator and short track). One member of each team competes in each stage with the exception of the road race where both cyclists compete.

Two mixed BMX events (racing and freestyle park) will take place with each team containing one boy and one girl. The freestyle park event will contain a


warranties, and if a court requires any
		other Contributor to pay any damages as a result, the Commercial
		Contributor must pay those damages.

		5. NO WARRANTY

EXCEPT AS EXPRESSLY SET FORTH IN THIS AGREEMENT, THE PROGRAM IS
		PROVIDED ON AN "AS IS" BASIS, WITHOUT WARRANTIES OR
		CONDITIONS OF ANY KIND, EITHER EXPRESS OR IMPLIED INCLUDING, WITHOUT
		LIMITATION, ANY WARRANTIES OR CONDITIONS OF TITLE, NON-INFRINGEMENT,
		MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Each Recipient is
		solely responsible for determining the appropriateness of using and
		distributing the Program and assumes all risks associated with its
		exercise of rights under this Agreement , including but not limited to
		the risks and costs of program errors, compliance with applicable
		laws, damage to or loss of data, programs or equipment, and
		unavailability or interruption of operations.

		6. DISCLAIMER OF LIABILITY

EXCEPT AS EXPRESSLY SET FORTH IN THIS AGREEMENT, NEITHER
		RECIPIENTindoors in a barn with single farrowing pens during their first parturition. During their second parturition, sows were kept outdoors in farrowing huts (group=INOUT). The behaviour was video recorded from 2 h prepartum to 48 h postpartum. The sows' responsiveness to playbacks of a piglet's screams was tested on days 2 to 3 postpartum. Parity 1: during the last 2 h prepartum, OUTOUT sows had a higher proportion of observations in the sternal lying position (P<0.01). During parturition, OUTOUT sows changed posture more often (P<0.05) and were lying less (P<0.05) than INOUT sows. All sows in both groups responded with 'lifting head' towards the playback of piglet scream, whereas 100% of OUTOUT sows and only 43% of INOUT sows thereafter were 'getting up' (P <0.01). Parity 2: There


[Character of the methylation and reassociation of wheat DNA fractions differing in their nucleotide composition].
The GC and AT fractions (GC content is 55.2 and 36.2 mole %, respectively) were isolated from wheat germ DNA by the thermal fractionation method (sequential thermal DNA denaturation followed by separation of denatured and undenatured DNA molecules by hydroxyapatitie chromatography). The amount of each of the two fractions isolated corresponds to about 25% of the total DNA. The 5-methylcytosine (m5C) content in the GC fraction (9.2 mole %) is 4.6 times higher than in the AT fraction. The DNA fractions isolated are very different in the level of methylation of cytosine residues (m5C/C+m5C ratio differs 3 times), in the amount of respective pyrimidine isostichs and in the renaturation kinetics. In contrast to thewhat forced the federal government to the negotiating table to give us the Civil and Voting Rights Acts. If Dr. King were alive today, I have a hunch the mall would be filled with protestors on Inauguration Day to protest the disgraceful nomination of Sen. Sessions.

It wasn’t “noble” for German/Jewish veterans of World War I to wear their Iron Crosses as they were rounded up to go to the concentration camps. It was pathetic.

I heard an interview with the college president today and they applied to march BEFORE the election so they were applying to march, not to march for one particular person. He also said that many of their students have never “had the opportunity to visit our nation’s capitol” and he saw this as a way


90*l. Let o = -20 - l. Suppose o = -g, -3*g - 13 - 2 = -z. Solve z = -i + 4 + 1 for i.
5
Let o(c) = 12*c**3 - 610*c**2 - 101*c + 19. Let n be o(51). Solve -36*x = -22*x + n for x.
-5
Suppose -f - 242 - 7 = -2*s, 2*s + 4*f = 264. Solve -s = 33*b - 12*b for b.
-6
Let b = -769 - -802. Solve 7*k = -b + 75 for k.
6
Let j(o) = -4*o**2 - 213*o - 602. Let g be j(-50). Solve 3*b = g - 24 for b.
8
Let v(b) = -b**2 - 17*b - 26. Let n be v(-6). Let i be n/140 + 124/7. Suppose 0 = -j - 5*j + i. Solve 5b = 292 - 264. Solve -b*x = -35*x - 21 for x.
-3
Let j = -1078 - -1084. Solve 45*f + j = 51*f for f.
1
Let q be (-187)/(-7) + 24/84. Let p = q - 19. Suppose p*n - 3*n = 0. Solve n = 2*b + b for b.
0
Let i(o) = 2*o**3 - 12*o**2 + 12*o. Let g be i(5). Let u be (3/5)/(1/g). Solve 3*n = n - u for n.
-3
Let d be 125/45 - 2/(-9). Suppose -6 + d = z - h, -3*z - 6 = -2*h. Let f be z - ((0 - 0) + -12). Solve -4*m = -m - f for m.
4
Suppose 5*j + 3*f = 186, -3*j + f + 149 = 29. Let c = 12 + j.


be i(12). Suppose -y*p + p = -42. Suppose -4*s = -2*s - p. Solve -s*j = -7*j for j.
0
Let l(n) = 65*n + 866. Let v be l(-13). Solve -v = 5*b - 1 for b.
-4
Let c = 6 - 3. Let o be 4*((-6)/(-15) + (-29)/10). Let d(g) = g**3 + 10*g**2 - 3*g - 28. Let p be d(o). Solve -c*k = 1 + p for k.
-1
Let i = 64 + -62. Suppose 3*u = i*m - 3, -2*m = 2*u + 3*u - 11. Let h = m - 0. Solve 2 = n - h for n.
5
Suppose -53 = -1173*u + 1172*u. Solve 0 = -41*r + u*r + 72 for r.
-6
Let r(l) = -5*l + 17. Let t be r(0). Suppose 12*vQ:

How to clear the mapview overlay drawing in android?

I am doing one small GPS application. I have to show multiple palces . I can able to show multiple places using custom overlay method.Now i have to show route between two points , while showing route i have to show only that two points. remaining all points should disappear but i dont know how to disappear the other points.

A:

This will surely work..
TestedAndVerified
      if(!mapOverlays.isEmpty())
     {
          mapOverlays.clear();
          mapView.getOverlays().clear();
          mapView.invalidate();
     }


Factors affecting recovery of Dictyocaulus viviparus third stage larvae from herbage and growth of Pilobolus on dung pats.
Significantly greater Dictyocaulus viviparus stage three larval recoveries from herbage samples adjoining first stage larval infected dung pats with Pilobolus, and the effects of biotic and mechanical factors on dung pat integrity supported previous findings. Several meteorological factors including sunshine hours, relative humidity, rain total and wind speed showed significant correlations with growth of Pilobolus on dung surfaces. The influence of monitored variables on the recovery of third stage larvae from herbage and the growth of Pilobolus was modified by height of the sward surrounding the dung pat. Multiple regression analysis showed that 60 per cent of variation in D viviparus third stage larvae recovery from herbage was accounted for bySales table, click and hold on the Ship Date column, then drag the cursor to the Date column in the Date table, and then release.

A dotted line appears showing you have created an inactive relationship between the Ship Date column in the Internet Sales table and the Date column in the Date table.


points to an inconsistency in the hypothesis of common etiology for these malformations. Whites were also found to have significantly higher incidences over Negroes of pyloric stenosis, congenital dislocation of the hip, micrognathia, and pectus excavatum, while Negroes have higher incidences of metatarsus varus and inguinal hernia.been fully realized.

The spec also comes with an example

Wrong specification. The ARB_timer_query specification is for desktop OpenGL; the EXT_disjoint_timer_query is for OpenGL ES. Don't confuse the two.
The EXT_disjoint_timer_query specification's examples seem to have been incorrectly copied from ARB_timer_query.


temperatures 15-20°C and, after reaching the optimum around *T*~*a*~ = 21°C, the difference between its *T*~*b*~ and *T*~*a*~ decreased again ([Fig 2A](#pone.0150393.g002){ref-type="fig"}; compare the fitted curve to the dashed line denoting *T*~*b*~ = *T*~*a*~). It seems that *E. aethiops* thus has a narrow temperature range for activity compared to the other species. Contrary, *E. medusa* had thermoregulatory characteristics similar to the alpine species ([Fig 2](#pone.0150393.g002){ref-type="fig"}). These species increased their *T*~*b*~ more at low than at high *T*~*a*~, which suggests active thermoregulation by microclimate choice or body posture adjustment under low air temperatures.

10.1371/journal.pone.0150393.t002

###### Body temperature depends on air and microhabitat temperatures.

Results of generalized additive models (GAM) testing the dependence of body-to-air temperature excess *T*~*b*~ − *T*~*a*~ on air temperature *T*~*a*~ and body-to-microhabitat temperature excess *T*~*b*~ − *T*~*m*~ on microhabitathave the possibility to retreat to the shade in case the weather gets too hot. Whereas mountain landscapes provide heterogeneity by their rugged terrain, lowlands are more prone to landscape homogenization and suffer from higher human pressure \[[@pone.0150393.ref062]\]. Lowland species living in woodlands, such as *E. aethiops* \[[@pone.0150393.ref018]\], may thus be more endangered by a combination of landscape homogenization and climate change than alpine species or species of open grasslands, such as *E. medusa*. They might be both more sensitive to high temperatures because of their lower preferred temperatures \[[@pone.0150393.ref014]\] and have limited opportunities for microhabitat shifts, e.g. more intensive shade seeking would be difficult because they already inhabit relatively cold microhabitats \[[@pone.0150393.ref021]\]. On the other hand, it could be expected that under climate warming, a grassland species


session though. I've been in to anal play before so I'm quite comfortable with the concept. And I'm sure I will learn to appriciate both models...

To the contrary, I've found the Helix MORE mobile than the MGX for precisely the reason that you site. The ribbing at the bottom of the MGX affords more resistance....the HELIX is more slippery in that department. That said, the Helix is larger in the body which can make for a tighter fit for some, ergo: more resistance. Once again, it depends on the individual to some extent. Regardless, they are both great units and should have a good time with either.

I found the MGX to be much more mobile than the helix, have atually stopped using the helix, I think it isone) was to address more specific needs. I had made the assertion early on that I felt that the Helix offered more intense contact up front, which I'd believed could translate into a shorter learning curve. Some responses have supported this while others have related having better luck with the Maximus or staying with the MGX.

While I understand the arguments made by the proponents of the MGX Classic (which I'm still a big fan of) that it is more responsive, (being that it is not as wide as the Helix and less contoured and has a more tapered base....a feature which is offset to a certain extent by the presence of the ribs on the base), the same cannot be said for the Maximus. This unit is without


a doubt the widest device (overall) that HIH produces, and features a very blunt tip (it was nicknamed the hammer by the Aneros staff for a time, because it resembles a ball peen hammer). As I've stated before, I found this model somewhat sluggish and out of balance. That said, I'm loathed to argue with anyone else's success with it,... as a matter of fact, I celebrate it! I've used it as a great warm-up and have actually had several nice orgasms with it, but it would not be my first choice as a sole unit.

So which one to get? If price was not an issue I'd say to get both the MGX Classic and the Helix.

If it must be one, the conventional wisdom would be to gopublic boolean onCreateOptionsMenu(Menu menu) {
        // Inflate the menu; this adds items to the action bar if it is present.
        getMenuInflater().inflate(R.menu.main, menu);
        return true;
    }

     // Adds food group data
    private void prepareListData() {
        listDataHeader = new ArrayList<String>();
        listDataChild = new HashMap<String, List<String>>();

        // Adding child data groups
        listDataHeader.add("Produce");
        listDataHeader.add("Grains");
        listDataHeader.add("Meat & Seafood");


   else if (choice.equals("Clear All Items")) {
                        Toast.makeText(getApplicationContext(), "All list items deleted.",
                                Toast.LENGTH_SHORT).show();
                    }
                    else if (choice.equals("Delete List")) {
                           return false;
                }
            });
            EditMenu.show();
        }

        // Create List Dialog
        public AlertDialog.Builder dialogBuilder;
        private void createListDialog() {
            dialogBuilder = new AlertDialog.Builder(this);
            final EditText textInput = new EditText(this);


              Toast.LENGTH_SHORT).show();
                }
            });
            dialogBuilder.setNegativeButton("Cancel", new DialogInterface.OnClickListener() {

                @Override
                public void onClick(DialogInterface dialog, int which) {
                    Toast.makeText(getApplicationContext(), "Cancelled.",
              });
            dialogBuilder.setNegativeButton("Cancel", new DialogInterface.OnClickListener() {

                @Override
                public void onClick(DialogInterface dialog, int which) {
                    Toast.makeText(getApplicationContext(), "Cancelled.",
                            Toast.LENGTH_SHORT).show();
                }


is beautiful; but, better than that, much better, she has
no thought of it herself.

She sits in front of her low cottage home singing a soft sweet song,
weaving a garland of scarlet flowers to adorn her head. As she carefully
places each bud on the string, she looks up at the American flag
floating in the breezes not far away.

The schoolmaster of the village tells her it is in honour of George
Washington, the greatest man of the United States; that if he had not
lived, America would not be what she is to-day, and she might not have
been able to give Hawaii the help needed when trouble came.

But what cares little Auwae for all this? What difference does it make
to her that her island home, the land of beauty and of flowers,STORY OF THE BEAR

=The Little People of the Sycamore.=

THE STORY OF THE RACCOON

_By OTHER AUTHORS_

=The Great Scoop.=

_By MOLLY ELLIOT SEAWELL_

A capital tale of newspaper life in a big city, and of a bright,
enterprising, likable youngster employed thereon.

=John Whopper.=

The late Bishop Clark's popular story of the boy who fell through the
earth and came out in China, with a new introduction by Bishop Potter.

=The Dole Twins.=

_By KATE UPSON CLARK_

The adventures of two little people who tried to earn money to buy
crutches for a lame aunt. An excellent description of child-life about
1812, which will greatly interest and amuse the children of to-day,
whose life is widely different.

=Larry Hudson's Ambition.=

_By JAMES OTIS_, author of "Toby Tyler," etc.

Larry Hudson is a typical American boy, whose hard work and enterprise
gain him his ambition,--an education and


called “the happy conspiracy” between corporate managers, directors, accountants, investment bankers, and institutional owners and renters of stocks, all kinds of bizarre financial engineering took place. The reported numbers met the demands of the expectations market, but often had little to do with the realities of the business market. Loose accounting standards made it possible to create, often out of thin air, what passes for earnings, even under GAAP standards.

My favorites, as it were, include hyping the assumed future returns earned by the pension plan, even as rational expectations for future returns deteriorated; post-merger accounting that creates a veritable “cookie jar” of reserves to be drawn on to create illusory earnings growth later on, even as we learn that some 61 percent of corporate mergers actually destroy shareholderour stock brokers, investment bankers, and money managers), even as any economically-strong society depends on the continuing creation of intrinsic value in our corporate world.

I raised this same issue rather more tartly in a speech I gave at Princeton University’s Center for Economic Policy Studies in 2002, the core ideas of which in turn found their way to a prominent role in my Battle book. The theme of that speech—entitled “Don’t Count On It. The Perils of Numeracy”—was that “in our society, in economics, and in finance, we place too much trust in numbers. But numbers are not reality. At best, they’re a pale reflection of reality. At worst, they’re a gross distortion of the truths we seek to measure . . . (Yet) we worship hard numbers


and accept the momentary precision of stock prices rather than the eternal vagueness of intrinsic corporate value as the talisman of investment reality.”

The Real Market and the Expectations Market

Perhaps this distinction between the Real Market and the Expectations Market was best expressed by Roger Martin, dean of the Rotman School of Business at the University of Toronto. Just think about it: In the Real Market of business, real companies spend real money and hire real people and invest in real capital equipment, to make real products and provide real services. If they compete with real skill, they earn real profits, out of which they pay real dividends. But to do so demands real strategy, real determination, and real capital expenditures, to say nothing of requiring real innovation andthe hard." "Only the strong." "We march for our lands, for our families, for our freedoms." "We march." "Daxos." "What a pleasant surprise." "This morning's full of surprises, Leonidas." "We've been tricked." "Can't be more than a hundred." "This is a surprise." "Silence." "This isn't their army." "We heard Sparta was on the warpath and we were eager to join forces." "If it is blood you seek, you are welcome to join us." "But you bring only this handful of soldiers against Xerxes?" "I see I was wrong to expect Sparta's commitment to at least match our own." "Doesn't it?" "You, there." "What is your profession?" "I'm a potter, sir." "And you, Arcadian." "What is your profession?" "Sculptor, sir." "Sculptor." "And you?" "Blacksmith." "Spartans!" "What is your profession?"


also not used. Please explain me the reasons for this behavior, and there is a possibility to specify hint of indices to be used for the shortest path, if I know for sure when planning data model?

A:

For filter conditions combined with logical ANDs as in your query, ArangoDB's query optimizer will pick a single index. This is the reason why it hasn't picked the edge index and the skiplist index at the same time.
It will do a selection between the skiplist index on expired and the edge index on [ "_from", "_to" ], and will pick the one for which it determines the lower cost, which is measured by index selectivity estimates. As the explain output shows, it seems to have picked the edge index on _to.
Theao mesmo tempo.
Assim:
session_start();

$email = 'email_user@site.com';

if(isset($_SESSION['user'])){

    $valor_da_sessao = $_SESSION['user'];

    $query = mysqli_query($conexao, "SELECT * FROM sessao WHERE valor = '$valor_da_sessao'");

    if(mysqli_num_rows($query) == 0){

        /*

            ESSA SESSÃO NÃO É ORIGINAL

        */

        unset($_SESSION['user']);
        session_destroy();

    } else {

        /*

            ESSA SESSÃO É ORIGINAL
            CRIA UM NOVO VALOR


either way, so won't
comment on it.

Have you ever talked to the left supporters yourself? They are the most racist
group I've ever met. Youtube is full of SJWs blaming white people for all the
problems, only because they are white. The are now protesting and writing
things like "Kill all the whites" on the walls. That's exactly what made me
distance myself from the left.

And just to clarify, I don't support Trump, I don't like him, but for much
different reasons than your made up ones.

~~~
dragonwriter
> "Mexican" is not a race.

Yes, it is "a family, tribe, people, or nation belonging to the same stock".
[0]

It's not one of the arbitrarily-defined classical races (negroid, caucusoid,
mongoloid) or one of those recognized as a "race" by the US census (which uses
the classic races essentially, with new names, The Hercules also saw use in civilian designs, culminating in the 735 and 737 engines for such as the Handley Page Hastings C1 and C3 and Bristol Freighter. The design was also licensed for production in France by SNECMA. Except for the 2000 hp-range versions in the 1950s until there were lubricating oil improvements, it was considered to be one of the more reliable aircraft engines of the era.

A total of over 57,400 Hercules engines were built.

Applications

Note:
Armstrong Whitworth Albemarle
Avro Lancaster B.II
Avro York C.II
Bristol Beaufighter
Bristol Freighter
Bristol Superfreighter
Breguet 890 Mercure
CASA C-207 Azor
Fokker T.IX
Folland Fo.108
Handley Page Halifax
Handley Page Hastings
Handley Page Hermes
Nord Noratlas
Northrop 8A (One Swedish 8A-1 was bought by Bristol to test the engine)
Northrop Gamma 2L
Saro Lerwick
Short S.26
Short Seaford
Short Solent
Short Stirling
Vickers Valetta
Vickers Varsity
Vickers VC.1 Viking
Vickers Wellesley
Vickers Wellington

Specifications (Hercules II)

See also

References

Notes

Bibliography

Gunston, Bill.


everyday from my home in Whiterun to his work south of Windhelm a distance several days in the making in game time, hence he is rarely home. The game is full of wholes that make the fiction less convincing, and writing that can be at times pathetic, but what it excels at around level 20 is a sense of genuine empowerment. Your dragonborn becomes a kinda god, able to take out dragons in a few bounds, cast spells in some cases infinitely and other acts of remarkable… questing. This is what Skyrim really is, an ego trip. The mechanics aren’t really that fun, Fable and the up coming Kingdoms of Amalur appear to be working towards a more satisfying sense of combat. The Assassin’s Creed games have madea forum of women playing through the game, but my little khajit mage and her argonian husband appear to be abnormalities in the geography of Skyrim’s possibilities (the game does try to power down mages apparently). Anyway, it’s a full trip and I’m grinding a few more levels before I don a wedding dress and wreath, enchant them up to their full potential and ride the back of a dragon to the final battle. All of which sounds exciting, but by this point it seems inane.

Saint’s Row the Third is another open world game which I actually snagged here in Thailand and then registered on steam. The above video about gets the possibility of the game. Saint’s Row the third isn’t about becoming the way Skyrim is, rather


the tutorial this is quite easy, but in the free form mode it’s quite hard. In the first game you simply click on the favoured boy and flirt with him, he flirts back and mission accomplished. You have a set amount of time to wonder through dialogue bubbles and find the right words of the job, it actually bears a similarity with Skyrim in this regard, in one mission in Skyrim for instance I had to convince a monk to come back to a cave with me so my guests could eat him. I failed to persuade him so in the end I had to bribe him. Prom Week takes this basic idea to a higher level. You see you can click on anyone and get them toassertStringRemove(replace);
        assertStringRemove(stringUtilsRemove);

        profileStringRemove(replaceAll, input, exclude, iterations);
        profileStringRemove(replace, input, exclude, iterations);
        profileStringRemove(stringUtilsRemove, input, exclude, iterations);

    }

    private static void assertStringRemove(StringRemove stringRemove) {
        String[] exclude = { "one", "two", "three" };
        String input = "if we add one and two we get three";
        String replaced = stringRemove.remove(input, exclude);

        String expected = "if we add  and  we get ";
        if


String remove(String input, String... subStringsToRemove) {
        for (int ix = 0; ix < subStringsToRemove.length; ix++) {
            input = StringUtils.remove(input, subStringsToRemove[ix]);
        }
        return input;
    }

}

The result on my computer is:
StringReplaceAll took: 3456 ms for 1000000 iterations
StringReplace took: 3162 ms for 1000000 iterations
StringUtilsRemove took: 761 ms for 1000000 iterations

A:

You can loop on the array and replace each String that appears in the input from it with empty String:
for(String str : exclude){
    input = input.replace(str, "");
}

A:

Without StringUtils you could implement it like this:
String[] exclude = {"one","two","three"};
String input = "if we add one andtestimony and therefore the sentencing process was not fundamentally unfair.
Finally, we will address whether the sentence of death was a product of passion, prejudice, or any other arbitrary factor; 42 Pa.C.S. 9711(h)(3). We have engaged in a careful review of the trial record. Based upon that review, we conclude that the sentence of death in Appellant's case was not the product of passion, prejudice, or any other arbitrary factor. Rather, the sentence was based upon the evidence admitted at trial and in compliance with the statutory mandate for the imposition of a sentence of death where one or more aggravating circumstances are found to outweigh any mitigating circumstances. 42 Pa.C.S. § 9711(c)(1)(iv). Furthermore, we have determined that the evidence was sufficient to support the aggravating circumstances the jury


v. Means, 565 Pa. 309, 773 A.2d 143, 162-67 (2001) (Nigro, J., dissenting). Nonetheless, I recognize that a majority of this Court has concluded otherwise, and under that binding precedent, I must agree that the trial court did not abuse its discretion in admitting victim impact testimony during the penalty phase of Appellant's trial.
NOTES
[1]  See 42 Pa.C.S. §§ 722(4), 9711(h)(1); Pa.R.A.P. 702(b) and 1941.
[2]  18 Pa.C.S. § 2501.
[3]  18 Pa.C.S. § 901.
[4]  18 Pa.C.S. § 3121.
[5]  18 Pa.C.S. § 3503.
[6]  18 Pa.C.S. § 3921.
[7]  42 Pa.C.S. § 9711(a).
[8]  We held in Commonwealth v. Grant 572 Pa. 48, 813 A.2d 726 (2002) that defendants are to reserve claims of ineffective assistance of trial counsel until collateral review proceedings. However, our reviewQ:

Laravel redirect inside of middleware?

I have a middleware check by login, how many games has the user, I want to show  another view if user have more than one game, but I got everytime redirect error if user has more than one:
This is in case $assigned_games > 1
$games = Auth::user()->userGames;
$assigned_games = count($games);

if ($assigned_games == 1) {
    return $next($request);
} elseif ($assigned_games > 1) {
    return redirect()->route('games.board');
} elseif ($assigned_games < 1) {
    echo "no game bought";
    exit;
}

I tried also only with return route('games.board') but it don't work.
How can I set correct redirect here?

A:

if both your route is inside middleware then you should check the current route and if its same as you are redirecting you shouldn't redirect


  Plant    R2II

\(a\)

All isolates were from Morocco.

\(b\)

Race assignation by multiplex specific-PCR amplifications, as described in

Gómez-Lama Cabanás et al. (2012)

. R1t = race 1 type, R2II = race 2 molecular group II.

dsRNA Purification {#S2.SS2}
------------------

Viral dsRNA was purified by cellulose column chromatography ([@B53]) from the mycelium collected after 7 days of growth in potato dextrose broth, as previously described ([@B32]). The purified dsRNA extracts were analyzed by electrophoresis in 0.8% agarose gels stained with RedSafe^TM^ Nucleic Acid Staining Solution (iNtRON Biotechnology, Seongnam-si, South Korea). The dsRNA nature of the extracts was confirmed by digestion with DNase I and RNase A treatment in low salt condition.

cDNA Synthesis, Cloning, Sequencing, and Phylogenetic Analysis {#S2.SS3}
--------------------------------------------------------------

The complete sequence of the viral RNA was determined using a combination of techniques asconfirmed by dsRNA extraction and RT-PCR. In addition, efficiency in virus transmission through the spores in the new infected isolate *Fod* 77Hyg^R^ was also determined.

Effect of FodHV2 on Selected Phenotypic Traits of Its Fungal Host {#S2.SS6}
-----------------------------------------------------------------

The effect of FodHV2 on its fungal host was analyzed by determining differences in mycelial growth rate between the virus-free (*Fod* 77Hyg^R^) and the virus infected (*Fod* 77Hyg^R^HV^+^) isolates on solid medium, conidiation rate in liquid medium, and virulence on carnation. To determine the effect of FodHV2 on mycelial growth rate, 100 conidia of each isolate were placed in the center of a PDA plate (three replicates), and the growth area was measured daily as previously described ([@B32]). To analyze the effect of FodHV2 on the conidiation rate in liquid medium, 200 conidia/ml


8,988. This sequence encodes a polyprotein of 2,892 aa residues with a predicted molecular mass of 268 kDa. The deduced aa sequence of the polyprotein contains highly conserved domains of UDP glucose/sterol glucosyltransferase (UGT), RNA-dependent RNA polymerase (RdRp), and viral RNA helicase (Hel). In addition, conserved Cys (Cys^339^), Hys (Hys^400^), and Gly (Gly^423^) residues in the N-terminal region of the polyprotein could represent the catalytic site of a highly diverged papain-like protease ([Figure 2B](#F2){ref-type="fig"}).

The UGT domain (nt 1,666--2,925) is 420 aa in length. Alignment of the aa sequence of this UGT domain with that of other mycoviruses in the *Hypoviridae* family identified the four highly conserved motifs (CM-1 to 4; [Figure 3](#F3){ref-type="fig"}) as previously described ([@B56]). Downstream from the UGT domain (nt 5,785--6,707) a 308 aa long RdRpindicated with a black line and his acronym. **(B)** Phylogenetic trees based on the RdRp and Hel aa sequences of FodHV2/408 and the selected hypoviruses. Phylogenetic trees were constructed using the program Tree View of Geneious 8.1.5 package (Biomatters), and generated by the NJ method with 1000 bootstrap replicates. Plum pox virus (PPV) was used as an out-group.](fmicb-10-03131-g004){#F4}

Phylogenetic Relationship Between FodHV2 and Other Hypoviruses {#S3.SS3}
--------------------------------------------------------------

To understand the phylogenetic relationship between FodHV2 and other members in the family *Hypoviridae*, a phylogenetic analysis based on the Neighbor-Joining method was performed using the full-length aa sequence of the viral polyprotein. Result obtained distributed hypoviruses into the three distinct phylo groups corresponding to proposed genera of "Alphahypovirus," "Betahypovirus," and "Gammahypovirus" ([@B59]; [@B25]; [@B28]; [@B36]), with FodHV2 grouping in the "Betahypovirus" clade ([Figure


was transferred), and RT-PCR products obtained using these dsRNA extracts and specific primers for the RdRp segment of FodHV2. M: molecular weight marker II (Roche Diagnostics). **(B)** Colony morphology of F*od* 77Hyg^R^ and *Fod* 77Hyg^R^HV^+^ after 9 days of growth on PDA. **(C)** Two dimensional colony growth rate of *Fod* 77Hyg^R^ and *Fod* 77Hyg^R^HV^+^. The colony area was measured at 3, 5, 7, and 9 days of growth on PDA with hygromycin at 25°C in the dark. Values are the average area of five colonies. **(D)** Conidiation rate of *Fod* 77Hyg^R^ and *Fod* 77Hyg^R^HV^+^ in liquid medium. Isolates were cultured in casein hydrolyzed medium, and conidia counted at 1, 2, 3, 4, 5, and 6 days of growth. Vertical lines in C and D represent the standard error.](fmicb-10-03131-g006){#F6}

Effectof FodHV2 on host growth on solid medium and conidiation rate in liquid medium was estimated by comparison of both *Fod* 77Hyg^R^ and *Fod* 77Hyg^R^HV^+^ isolates. Results obtained showed that neither the morphology and radial growth rate on solid medium, nor the conidiation rate in liquid medium, were significantly affected by the FodVH2- infection ([Figure 6](#F6){ref-type="fig"}).

To determine the effect of FodHV2 on virulence, two pathogenicity tests were performed in which several susceptible carnation cultivars were inoculated with each *Fod* 77Hyg^R^ and *Fod* 77Hyg^R^HV^+^ strains. Factorial analysis of the disease data showed that there were no significant differences (*P* ≤ 0.05) between the sAUDPC percentages obtained with all the susceptible carnation cultivars and the two inoculated strains ([Table 4](#T4){ref-type="table"}). Fungal isolation assays performed with representatives of plants inoculated with


and for which a creation of a new genus ("Gammahypovirus") has been recently proposed ([@B25]). Genome length, genomic organization, and phylogenetic analysis, clearly indicate that FodHV2 could be assigned to the "Betahypovirus" group. As in other possible members in that putative genus, genome of FodHV2 is shorter than that of the members in the Alpha- or Gamma- groups, and contains a single ORF. The encoded polyprotein includes protease, RdRp, and helicase domains, characteristic of all hypoviruses, but also contains a distinctive UGT domain which is encoded solely by the members of the "Betahypovirus" group ([@B59]; [@B15]). In addition, viruses in the putative genus "Betahypovirus" share sequence conservation in the 5′-UTR, including a 15-nt sequence (CTGGTTTATACTCTG) present in all known putative betahypoviruses ([@B15]). The comparison of the complete nt                              reference;
    typedef const value_type&                                          const_reference;
    static_assert((is_same<value_type, typename allocator_type::value_type>::value),
                  "Invalid allocator::value_type");

private:
    typedef __hash_table<value_type, hasher, key_equal, allocator_type> __table;

    __table __table_;

public:
    typedef typename __table::pointer


key_equal& __eql,
                      const allocator_type& __a);
#if _LIBCPP_STD_VER > 11
    template <class _InputIterator>
    inline _LIBCPP_INLINE_VISIBILITY
        unordered_set(_InputIterator __first, _InputIterator __last,
                    size_type __n, const allocator_type& __a)
            : unordered_set(__first, __last, __n, hasher(), key_equal(), __a) {}
    template <class _InputIterator>
        unordered_set(_InputIterator __first, _InputIterator __last,
                     size_type __n, const hasher& __hf, const allocator_type& __a)
            : unordered_set(__first, __last, __n, __hf, key_equal(), __a) {}
#endif
    explicit unordered_set(const allocator_type& __a);
    unordered_set(const unordered_set& __u);
    unordered_set(const unordered_set& __u, const allocator_type& __a);
#ifndef _LIBCPP_HAS_NO_RVALUE_REFERENCES
    unordered_set(unordered_set&& __u)
        _NOEXCEPT_(is_nothrow_move_constructible<__table>::value);
    unordered_set(unordered_set&& __u, const allocator_type& __a);
#endif  // _LIBCPP_HAS_NO_RVALUE_REFERENCES
#ifndef _LIBCPP_HAS_NO_GENERALIZED_INITIALIZERS
    unordered_set(initializer_list<value_type> __il);
    unordered_set(initializer_list<value_type> __il, size_type __n,
                  const hasher& __hf = hasher(),


   __get_db()->__insert_c(this);
#endif
    __table_.rehash(__n);
    insert(__first, __last);
}

template <class _Value, class _Hash, class _Pred, class _Alloc>
template <class _InputIterator>
unordered_multiset<_Value, _Hash, _Pred, _Alloc>::unordered_multiset(
        _InputIterator __first, _InputIterator __last, size_type __n,
        const hasher& __hf, const key_equal& __eql, const allocator_type& __a)
    : __table_(__hf, __eql, __a)
{
#if _LIBCPP_DEBUG_LEVEL >= 2
    __get_db()->__insert_c(this);
#endif
    __table_.rehash(__n);
    insert(__first, __last);
}

template <class _Value, class _Hash, class _Pred, class _Alloc>
inline _LIBCPP_INLINE_VISIBILITY
unordered_multiset<_Value, _Hash, _Pred, _Alloc>::unordered_multiset(
        const allocator_type& __a)
    : __table_(__a)
{
#if _LIBCPP_DEBUG_LEVEL >= 2
    __get_db()->__insert_c(this);
#endif
}

template <class _Value, class _Hash, class _Pred, class _Alloc>
unordered_multiset<_Value, _Hash, _Pred, _Alloc>::unordered_multiset(
       const unordered_multiset& __u)
    : __table_(__u.__table_)
{
#if _LIBCPP_DEBUG_LEVEL >= 2
    __get_db()->__insert_c(this);
#endif
    __table_.rehash(__u.bucket_count());
    insert(__u.begin(), __u.end());
}

template <class _Value, class _Hash, class _Pred, class _Alloc>
unordered_multiset<_Value, _Hash, _Pred, _Alloc>::unordered_multiset(
        const unordered_multiset& __u, const allocator_type& __a)
    : __table_(__u.__table_, __a)
{
#if _LIBCPP_DEBUG_LEVEL >= 2
    __get_db()->__insert_c(this);
#endif
    __table_.rehash(__u.bucket_count());
    insert(__u.begin(), __u.end());
}

#ifndef _LIBCPP_HAS_NO_RVALUE_REFERENCES

template <class _Value, class _Hash, class _Pred, class _Alloc>
inline _LIBCPP_INLINE_VISIBILITY
unordered_multiset<_Value, _Hash, _Pred, _Alloc>::unordered_multiset(
        unordered_multiset&& __u)
    _NOEXCEPT_(is_nothrow_move_constructible<__table>::value)
    : __table_(_VSTD::move(__u.__table_))
{
#if _LIBCPP_DEBUG_LEVEL >= 2
    __get_db()->__insert_c(this);
    __get_db()->swap(this, &__u);
#endif
}

template <class _Value, class _Hash, class _Pred, class _Alloc>
unordered_multiset<_Value, _Hash, _Pred, _Alloc>::unordered_multiset(


a vector subspace of ${\mathfrak}{h}$. When ${\mathfrak}{g}$ is compact (and only then), we have $\mathcal{C} = \{0\}$.

For Problem \[nontrivial\_w0\_action\], we only have experimental results so far. Explicit computation of ${{\left. w_0 \right|}_{V^{\mathfrak}{l}}}$ for all sufficiently low-dimensional representations $V$ of all sufficiently low-rank simple Lie algebras, combined with Theorem \[submonoid\_and\_ideal\].\[itm:ideal\], suggests the following generalization of Theorem \[LFlSm\]:

Let ${\mathfrak}{g}$ be any simple real Lie algebra of rank $r$, and $\lambda \in Q \cap {\mathfrak}{h}^+$ a dominant weight of ${\mathfrak}{g}$. Let us decompose $\lambda$ into its coordinates on the basis formed by the fundamental weights: $\lambda = \sum_{i=1}^r c_i \varpi_i$. Suppose that $V_\lambda^{\mathfrak}{l} \neq 0$: then ${{\left. w_0 \right|}_{V_\lambda^{\mathfrak}{l}}} = \pm \operatorname{Id}$ can happen only when at most $K$ of the coordinates $c_i$ can be nonzero, where $K = 3$.

(Compare this with the split case, where this statement holdswho should act as trainer. The incident is reported to the organization's managers, who agree that no further steps are needed to address the situation, accepting the harm as a kind of "collateral damage" for an otherwise useful program.

For the organization, the emphasis was on ensuring a continued presence in the country, accepting a certain degree of harm. However, it cannot simply be assumed that high-risk actions potentially resulting in significant harm to patients are justified for the sake of remaining present in certain contexts, at least if this means pursuing a political rather than a purely humanitarian goal. Programs that deploy highly qualified staff such as surgeons and have the goal of implementing projects bearing high risks to the affected population need to be thoroughly evaluated in


on the way forward

In a suitably adapted form, similarly structured multi-step approaches might also support the decision-making processes in humanitarian programs. In addition, clinical ethics has developed models for fostering expertise in clinical institutions without moral responsibility being delegated to an "ethics expert" (see "Institutional requirements" below).

**2.3. Identifying ethical issues and making decisions in humanitarian aid**

Our suggested ethical framework is an attempt to engage the humanitarian community in the discourse of identifying ethical issues in the context of humanitarian action and to provide tools to strengthen the decision-making process of humanitarian aid workers. The framework draws from previously developed concepts that have proven useful in public health and clinical ethics. This framework has three main aims.

First, it intends to emphazise that humanitarian action is based on *sets ofpromotes transparency of the decision-making process, which allows for a comprehensive monitoring and evaluation of humanitarian programs. Third, the framework formulates *institutional requirements*, drawing parallels to a tool that has proven useful in the clinical setting. Overall, the proposed framework has been based on the experiences of several humanitarian fieldworkers and was tested and deemed useful by humanitarian aid workers and donor agencies. This approach was designed so that it can evolve further and be adjusted to specific needs in the field.

**Part One: Defining a set of values guiding humanitarian action**

The values listed in Table 1 have been drawn from an empirical study by one of the authors that analyzed the value statements of 46 international organizations active in the humanitarian context[@ref25]. The ten most frequently mentioned values


(OS), progression-free survival (PFS), locoregional control (LRC), and clinical stage. EGFR and CD44 positivity (+) and p16 negativity (-) were associated with clinical stage IV of the disease. CD44+ and EGFR+ OPSCC displayed worse OS and LRC, and these cases also showed the worst 3-year OS and LRC. Combined analysis of protein expressions identified an association between p16- and EGFR+, p16- and CD44+, EGFR+, and CD44+. Combined immunoprofiles CD44+/p16-, EGFR+/p16-, and EGFR+/CD44+ were associated with worst OS and LRC. Combined immunoprofiles of p16, EGFR, and CD44 might provide valuable prognostic and predictive information for the individual OPSCC patients, especially in terms of response to IMRT and prediction of treatment outcomes. Application of the AJCC 8th edition staging for HPV+ OPSCC proved to improve hazard discrimination and prognostication of(a) Field of the Invention
The present invention relates to a plastic substrate having a multi-layer structure with a small coefficient of thermal expansion, excellent dimensional stability, and superior gas barrier properties and a method for preparing the same.
(b) Description of the Related Art
Although glass plates used for display devices, picture frames, craftwork, containers, etc. are advantageous in that they have a small coefficient of linear expansion, superior gas barrier properties, high transparency, good surface flatness, excellent heat resistance and chemical resistance, etc., they tend to break easily and be heavy because of their high density.
Recently, as liquid crystal displays, organic light emitting devices, and electronic paper are arousing a growing interest, research on replacing the glass substrates used in such devices with plastic counterparts is gaining momentum. The


plastic substrate is advantageous over the glass plate in terms of weight and ease of design. Also, because it is impact-resistant, an economic advantage may be attained from continuous manufacturing.
For a plastic substrate to be used in a display device, it should have a glass transition temperature high enough to endure the transistor processing temperature and the transparent electrode deposition temperature, oxygen and water vapor barrier properties so as to prevent aging of liquid crystals and organic light emitting materials, a small coefficient of thermal expansion and good dimensional stability so as to prevent deformation of the plate due to change of the processing temperature, mechanical strength comparable to that of the conventional glass plate, chemical resistance sufficient for enduring the etching process, high transparency, low birefringence, goodsupport, than the relatively short molecules. The length of the long polynucleotides can be 50 to 10,000 nucleotides in length, preferably 100 to 1000 nucleotides in length. If the relatively short molecules are not polynucleotides, then the relatively long polynucleotides are at least the equivalent physical distance of one nucleotide longer (or one nucleotide pair, if the polynucleotide is double stranded) than the relatively short molecules. The term “relatively long” also encompasses polynucleotides which extend above the relatively short molecules in an array format where the relatively long polynucleotides are distributed on the solid support at a density of about 106 to about 109 polynucleotides per cm2, and where the relatively short molecules are distributed at a density greater than about 108 to about 1014 molecules per cm2.


In general, the surface of the substrate is engineered so that the short molecules display a hydrophilic group from the surface. The relatively short molecules can therefore be silanes, amino acids, an acid, phosphate, thiophosphate, sulfate, thiol, hydroxyl or polyol, etc. and may include polyethers such as PEG. The types of molecules used will also depend on the surface chemistry used to attach the long molecules to the surface.

[0011]

As used herein, the term “single polynucleotide molecule” refers to one polymeric molecule of a nucleic acid sequence. Thus, an array feature or address corresponding to a single relatively long polynucleotide consists of one polynucleotide molecule immobilized onto a solid support. The immobilized single polynucleotide molecule can be single- or double-stranded, or have both single-stranded portions and double-stranded portions. Forcan also be of different lengths relative to each other, e.g., some of the polynucleotides may be 100 nucleotides in length, while others may be 120 nucleotides in length. By saying that each molecule of “at least a subset” of the plurality of first molecules is shorter in length than the length of each of the second polynucleotide of “at least a subset” of the plurality of second polynucleotides, is meant that one practicing the invention has arrayed polynucleotides that are intended to be physically longer (in that portion of the relatively long polynucleotide that is not used for attachment to the substrate or to a linker molecule(s) attached to the substrate) than the short molecules, but due to breakage of the polynucleotides or binding of short molecules


the present invention, it may be possible to image at least 106-109, preferably 107 or 108 long polynucleotides/cm2. Fast sequential imaging may be achieved using a scanning apparatus; shifting and transfer between images may allow higher numbers of polynucleotides to be imaged.

[0031]

The extent of separation between the individual polynucleotides on the array will be determined, in part, by the particular technique used to resolve the individual polynucleotide.

[0032]

Apparatus used to image molecular arrays are known to those skilled in the art. For example, a confocal scanning microscope may be used to scan the surface of the array with a laser to image directly a fluorophore incorporated on the individual polynucleotide by fluorescence. Alternatively, a sensitive 2-D detector, such as a charge-coupled device, can be used to provide a 2-Dof biological or chemical molecules with the arrays are carried out in solution.

[0060]

In particular, the arrays may be used in conventional assays which rely on the detection of fluorescent labels to obtain information on the arrayed polynucleotides. The arrays are particularly suitable for use in multi-step assays where the loss of synchronisation in the steps was previously regarded as a limitation to the use of arrays. The arrays may be used in conventional techniques for obtaining genetic sequence information. Many of these techniques rely on the stepwise identification of suitably labelled nucleotides, referred to in U.S. Pat. No. 5,654,413 as “single base” sequencing methods.

[0061]

In an embodiment of the invention, the sequence of a target polynucleotide is determined in a similar manner to that described in U.S. Pat. No.


(50 mL) was mixed for 5 min. The clean, dried slides were added to this mixture and left for 1 hour at room temperature rinsed with EtOH, dried and cured for 1 hour at 100° C. Maleimide modified DNA was prepared from a solution of amino-DNA (5′-Cy3-CtgCTgAAgCgTCggCAggT-heg-aminodT-heg-ACCTgCCgACgCT; SEQ ID NO:1) (10 μM, 100 μL) and N-[g-Maleimidobutryloxy]succinimide ester (GMBS); (Pierce) (1 mM) in DMF/diisopropylethylamine (DIPEA)/water (89/1/10) for 1 hour at room temperature. The excess cross-linker was removed using a size exclusion cartridge (NAP5) and the eluted DNA freeze-dried in aliquots and freshly diluted prior to use. An aliquot of the maleimide-GMBS-DNA (100 nM) was placed on the thiol surface in 50 mM potassium phosphate/1 mM EDTA (pH 7.6) and left for 12 hours at room temperature prior to washinglaser with a 580DF30 emission filter (Omega optics), with an exposure of 500 ms and maximum camera gain and a laser power of 50 mW at the prism.

[0071]

The presence of glycidoxypropyltrimethoxysilane gave improved results (FIG. 1a) compared to a control carried out in the absence of glycidoxypropyltrimethoxysilane.

Example 2

[0072]

Slides were cleaned with decon 90 for 12 hours prior to use and rinsed with water, EtOH and dried. A solution of tetraethoxysilane (0.7 mL) and N-(3-triethoxysilylpropyl)bromoacetamide (0.0007 mL) in acidified 95% EtOH (35 mL) was mixed for 5 minutes. The clean, dried slides were added to this mixture and left for 1 hour at room temperature, rinsed with EtOH, dried and cured for 1 hour at 100° C. Phosphorothioate modified DNA (5′-TMR-TACCgTCgACgTCgACgCTggCgAgCgTgCTgCggTTsTsTsTsT ACCgCAgCACgCTCgCCAgCg; SEQ ID NO:2) where s=phosphorothioate (100 pM,


difference was noted in the mean fraction of drug excreted as glucuronide between the two groups (51.5% in Caucasians vs 51.8% in Orientals). However, the data strongly indicated that the excretion of acetaminophen glucuronide was not normally distributed. Bimodality was apparent in both groups, with 20% of Caucasian and 33% of Oriental subjects displaying relatively extensive glucuronidation. In addition, glucuronidation displayed a strong negative correlation with sulfation (r = -0.97), suggesting the existence of a compensatory mechanism between the two metabolic pathways. The mean fractional excretions of cysteine and mercapturate conjugates did show significant differences between Caucasians and Orientals (p < 0.005). In addition, the ratio of mercapturate to total GSH-derived conjugates recovered appeared to be bimodal, indicating possible heterogeneity in the conversion of the cysteine conjugate towe conclude that the covariance gain modulation depends largely on the modulation of individual neurons.

Network requirements for attentional modulation {#s2-3}
-----------------------------------------------

Having described attentional modulation statistically our next goal is to develop a circuit model to understand the process mechanistically. Consider a network of $N$ coupled neurons, and let the spike count from neuron $i$ on a given trial be $y_{i}$. The network output has the covariance matrix $\mathbf{C}$ with elements $c_{ij} = {\text{Cov}{(y_{i},y_{j})}}$. In this section we identify the minimal circuit elements so that the attentional mapping $A_{\mathbf{C}}:\mathbf{C}^{U}\ \mapsto\ \mathbf{C}^{A}$ satisfies the following two conditions (on average):

**C1:** $c_{ij}^{A} = {g_{i}g_{j}c_{ij}^{U}}$ ; attentional modulation of covariance is rank one ([Figure 2](#fig2){ref-type="fig"}).

**C2:** $g_{i}\  < \ 1$ ; spike count covariance decreases with attention ([Figure 1](#fig1){ref-type="fig"}).

What follows is only a sketch


[@bib6]), particularly when inhibitory circuits are engaged ([@bib32]).

Attention can simultaneously increase stimulus gain and decrease noise covariance {#s2-6}
---------------------------------------------------------------------------------

An important neural correlate of attention is enhanced stimulus response gain ([@bib41]). The previous section outlines how the recruitment of recurrent inhibitory feedback by attention reduces response variability. However, inhibitory feedback is also a common gain control mechanism, and increased inhibition reduces response gain through the same mechanism that dampens population variability ([@bib69]). Thus it is possible that the decorrelating effect of attention in our model may also reduce stimulus response gain as well, which would make the model inconsistent with experimental data.

To insert a bottom-up stimulus $s$ in our model we let the attention-independent background input have a stimulus term: $\mu_{\alpha B} = {{k_{\alpha}s} + {\hat{\mu}}_{\alpha B}}$. Here $k_{\alpha}$ isthe excitatory-inhibitory cortical circuit. First, top-down attentional signals must affect inhibitory neurons more than excitatory neurons to allow a better damping of global fluctuations in the attended state. Second, bottom-up stimulus information must be biased towards excitatory cells to permit higher gain in the attended state. In total, the increased response gain and decreased correlations enhance the flow of information when the readout is confined to the excitatory population.

Candidate physiological mechanisms for attentional modulation {#s3-1}
-------------------------------------------------------------

Our model does not consider a specific type of inhibitory neuron, and rather models a generic recurrent excitatory-inhibitory circuit. However, inhibitory circuits in cortex are complex, with at least three distinct interneuron types being prominent in many areas: parvalbumin- (PV), somatostatin- (SOM), and vasointestinal peptide-expressing (VIP) interneurons ([@bib62]; [@bib52]; [@bib31]). In mouse visual cortex,


([@bib1]). In particular, the recruitment of VIP cell activity in vivo through BF stimulation is strongly dependent on both the muscarinic and nicotinic cholinergic pathways ([@bib1]; [@bib34]; [@bib21]), and it has thus been hypothesized VIP cells activation could be an important component of attentional modulation ([@bib1]; [@bib53]).

If we consider the inhibitory population in our model to be PV interneurons then the recruitment of VIP cell activity via top-down cholinergic pathways is consistent with our attentional model in two ways. First, activation of the VIP $\rightarrow$ SOM $\rightarrow$ pyramidal cell pathway provides a disinhibition to pyramidal cells, modeled simply as an overall depolarization to pyramidal cells in the attended state ([Figure 4](#fig4){ref-type="fig"}). Second, the activation of the VIP $\rightarrow$ SOM $\rightarrow$ PV cell pathway disinhibits PV cells, and thebe the matrix containing spike counts of the neurons on trials in which they are in the unattended state, and $S^{A}$ the matrix containing spike counts of the neurons on trials in which they are in the attended state. Denote the unattended spike count covariance matrix by $C^{U} = {Cov}(S^{U})$, and the attended one by $C^{A} = {Cov}(S^{A})$. Attentional changes in covariance and variance were measured both on average ([Figure 1c](#fig1){ref-type="fig"}) and as distributions ([Figure 1d](#fig1){ref-type="fig"}). The distributions of the normalized differences$$\frac{{Cov}^{A} - {Cov}^{U}}{{max}(|{Cov}^{A}|,|{Cov}^{U}|)}\quad\text{~and~}\quad\frac{{Var}^{A} - {Var}^{U}}{{max}(|{Var}^{A}|,|{Var}^{U}|)}$$

reveal a concentration of negative covariance changes, and a distribution of variance changes symmetric about zero. Here, ${Cov}^{A}$ and ${Cov}^{U}$ (${Var}^{A}$ and ${Var}^{U}$) are vectors containing covariance (variance) values of the entire data set. Note that the distributions are bounded between $- 2$


resulting in two estimates $g_{i}^{(1)}$ and $g_{i}^{(2)}$. (**b**) Spread of $g$ estimates for the data (black), as well as the upper (blue) and lower (green) bounds, from one day of recordings in one monkey. (**c**) Mean variance of the $g$ estimates computed from the data (abscissa) vs from the upper bound (ordinate), normalized by the mean shuffled variance. Each color denotes one of the monkeys, circles denote the right hemisphere recordings, and plusses denote the left hemisphere recordings. The gray regions consist of those points that are beyond $0.5$, and therefore closer to the lower bound than the upper bound.**DOI:** [http://dx.doi.org/10.7554/eLife.23978.016](10.7554/eLife.23978.016)

For each data set, the analysis is done for each neuron for $100$ different permutations of the neurons to generate $S_{i}^{(k)}$, $k = {1,\ldots,100}$. For shuffled and upper-boundthe attended state.

The spiking model also provides the opportunity to investigate the inputs to individual neurons, something that is difficult to do experimentally, and does not apply to mean-field models. [Appendix 1---figure 6k,l](#fig14){ref-type="fig"} shows the correlation functions of different types of inputs to a pair of excitatory neurons, averaged over pairs of excitatory neurons, in the unattended (k) and attended (l) states. Computing the correlation functions of the total recurrent input (black curves) reveals that correlations between excitatory inputs (EPSC-EPSC, dashed green), and correlations between inhibitory inputs (IPSC-IPSC, dashed red), are canceled by anti-correlations between excitatory and inhibitory inputs (EPSC-IPSC, blue). This is consistent with the idea of correlation cancellation by inhibitory tracking of excitatory activity ([@bib56]; [@bib70]; [@bib39]). Attention, by shifting the system into a more stable


I am, though, going to give it a shot. The exposition will be a little uneven, as I combined the reviews, without trying to edit for uniformity.*

*1) A big issue is robustness to parameters. Essentially what we want to know is: what are the constraints on parameter space for the results to hold qualitatively? It\'s probably hard to fully answer this, but it should be possible to provide answers for some of the more important parameters. Following are some more specific points.*

*First is a long comment about two of the modelling assumptions:*

*- The weights scale as 1/N.*

*- Perfect balance (J~EE~ = J~IE~ = J~E~ and J~II~ = J~EI~ = J~I~).*

The reviewers raise two excellent points and we outline our response to both of them below.

*This 1/N scaling iscovariance decreases with attention ([Figure 1](#fig1){ref-type="fig"}).

We will argue below that $1/\sqrt{N}$coupling will not affect how C1 is satisfied, but will technically prevent C2 (in the large N limit).

To establish C1 we first observe that the covariance C decomposes as:

Here the matrix K has element K~ij~ = *L~i~J~ij~* where *L~i~* is the linear response and *J~ij~* is the synaptic strength from neuron *j* to *i*. The issue at hand is that we require that the internally generated covariability vanish for large *N*. If the spectral radius of K is less than 1, then we can expand $\left( {I - K} \right)^{- 1} = I + \ \sum\limits_{n = 1}^{\infty}K^{n}$(Pernice et al., 2011; Trousdale et al., 2012). Inserting this expansion into the expression for the internally generated covariability yields:

(I −