ApoB levels increased in the livers of both tunicamycin-injected and HFD mice compared to the control mice. However, MEAO administration markedly decreased the hepatic ApoB levels. Together, these results suggest that MEAO restores the inhibition of ApoB secretion by ER stress.

![MEAO represses very low-density lipoprotein receptor (VLDLR) expression and increases apolipoprotein B (ApoB) secretion in tunicamycin (Tu) or palmitate (PA)-treated HepG2 cells, and in the livers of tunicamycin-injected or high-fat diet (HFD) obese mice. (**A**) q-PCR analysis of VLDLR in tunicamycin or palmitate-treated HepG2 cells. --, without tunicamycin or palmitate; +, with tunicamycin or palmitate. Values are expressed as the mean ± SEM (*n* = 3 independent experiments). ^\#^ *p* \< 0.05 compared with untreated control. \* *p* \< 0.05 compared with tunicamycin or palmitate-treated control; (**B**) q-PCRof the UPR regulate the transcription of various genes involved in the expansion of ER, decrease of protein synthesis, increase of protein folding capability and degradation of terminally unfolded and misfolded proteins within the ER. Previous study reported that IRE1α contributed to the development of hepatic steatosis through upregulation of gene expression involved in fatty acid and triglyceride synthesis by XBP-1 \[[@B14-ijms-16-25944]\]. PERK also increased the expression of the lipogenic enzymes such as FAS, ATP citrate lyase, and stearoyl-CoA desaturase-1 in the mouse mammary gland \[[@B15-ijms-16-25944]\]. Furthermore, CHOP deficiency in mice inhibited cholestasis induced liver fibrosis \[[@B24-ijms-16-25944]\]. According to our current results, MEAO blocked the increase in GRP78, CHOP and XBP-1x splicing respectively, suggesting that MEAO potentially acts on hepatic steatosis via inhibition of the three branches of


UPR.

To investigate the mechanism involved in ER stress-mediated inhibition of lipid accumulation, we first examined the expression of lipogenic genes in the livers of tunicamycin-injected mice because ER stress regulates lipid metabolism-genes. Consistent with previous reports \[[@B16-ijms-16-25944],[@B17-ijms-16-25944]\], tunicamycin treatment decreased the expression of lipogenic genes including FAS, ACC1, and GAPT despite the presence of hepatic steatosis. However, this was not significantly affected by MEAO. These results suggest that the rescue of tunicamycin-induced hepatic steatosis by MEAO was not due to the inhibition of lipogenesis. However, the increased expression of lipogenic genes in PA-treated HepG2 cells and in the liver of HFD obese mice was inhibited by MEAO, suggesting that MEAO potentially ameliorates fatty acid-induced hepatic steatosis through inhibition of lipogenesis.

Furthermore, it was known that VLDLR mediates APOE-containg VLDLwas supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (No. 2014R1A5A2009936).

######

Click here for additional data file.

Supplementary materials can be found at <http://www.mdpi.com/1422-0067/16/11/25944/s1>.

Min-Kyung Jang performed cell experiment, and Yu-Ran Han prepared MEAO, and Jeong Soo Nam performed animal experiment. Chang Woo Han, Byung Joo Kim, Han-Seol Jeong and Gi-Tae Ha are co-supervisors and members of the research team who contributed toward the supervision of the experiment and provision of experimental materials. Myeong Ho Jung designed the experiment, analyzed the data, and wrote the main manuscript text.

The authors declare no conflict of interest.

ACC1, Acetyl-CoA carboxylase1; CHOP, C/EBP homolog protein; ER stress, Endoplasmic reticulum stress; FAS, Fatty acid synthase; GRP78, Glucose regulated protein 78; HFD, High fat diet; IRE1α, Inositol-requiring kinase 1α;


Abortion decision making by focusing: a preliminary study.
In 2005, 28,738 Dutch women underwent an abortion. Of this group, 10% were in doubt whether or not to have their pregnancy terminated. The author wished to show that focusing facilitates the decision-making process in such cases. The article starts by describing the psychological consequences and accompanying problems of unplanned pregnancy. Thereafter the method of decision making by focusing is described. Two cases with verbatim transcription are given to illustrate the focusing process. The article ends with the presentation of the results of a small pilot survey concerning the method. The two case examples with verbatim transcriptions clarify the psychological process of decision making by using the method of focusing. The pilot survey shows that 16 of the 22 women (73%)a small region of the standard model parameter space [@James:1992wb], although the stable region may be enlarged when bound states are taken in to account [@Vachaspati:1992mk]. Because there are also differences between the 2SC phase of QCD and the Higgs phase of the standard model, a separate stability calculation will be needed for the 2SC case.

The 2SC phase contains three species of gapless fermions: two quarks (“blue up” and “blue down”) and the electron. These are expected to dominate its transport properties. Strange quarks can be included in the analysis as long as their pairing pattern does not break the ${{\tilde Q}}$ gauge symmetry. Muons may also be present, but, like strange quarks, their higher mass gives them a lower Fermi momentum so they make a sub-leading contribution to the


Expands Strategic Partnership to Accelerate Canopy Growth's Global Expansion Plans in Rapidly-Growing Medical and Recreational Cannabis Markets

VICTOR, NY and SMITHS FALLS, ON, Aug. 15, 2018 /CNW/ - Constellation Brands (NYSE: STZ) (NYSE: STZ.B), a leading beverage alcohol company, and Canopy Growth Corporation (Canopy Growth) (TSX: WEED) (NYSE: CGC), a leading diversified cannabis company (together, the "Companies"), today announced a significant expansion of their strategic partnership to position Canopy Growth as the global leader in cannabis production, branding, intellectual property and retailing.

Constellation Brands will increase its ownership interest in Canopy Growth by acquiring 104.5 million shares directly from Canopy Growth, thereby achieving approximately 38 percent ownership when assuming exercise of the existing Constellation warrants. Constellation Brands is acquiring the new shares at a price of C$48.60 per share, whichto any wedding planning on Thursday with this brief statement posted on its Facebook page:

Bert and Ernie are best friends. They were created to teach preschoolers that people can be good friends with those who are very different from themselves.

Even though they are identified as male characters and possess many human traits and characteristics (as most Sesame Street Muppets do), they remain puppets, and do not have a sexual orientation.

This is a more complete version of the story than the one that appeared in print.


C?  If a candidate told me "No, I am not a C expert", but gave me great answers to these other questions, I would hire them in a heartbeat over the guy who the magic 8-ball said was a C expert, but doesn't know how to check his code into subversion and hasn't learned a new language in 12 years.

A:

When I interviewed with Google, the interviewer told me to think about it this way.  On a scale of 1-10 for C proficiency, to say you're a "10" means you've written papers and/or books or been a speaker in a conference on programming in C.  Based on this, very few people are 10s.
FWIW, I have been programming in C for 15 years.  I consider myselfand $C$, as function of the native contacts and inter-protein contacts, for different protein concentration $c$. Native contacts have been normalized to 1 and inter-protein contacts have been normalized to $ln$, where $n$ is the number of proteins simulated and $l$ is the length (number of amino acids) of a single protein. In the shown cases, the size of the simulation box have been chosen such that $c=n$, i.e. a single protein occupies a volume corresponding to the 1% of the available volume.[]{data-label="F_Nc_Ic"}]({Histo_NATIVE-INTER_ProtB_c5.dat}.png "fig:")![Color map of the free energy profile of the protein $A_0$, $B$ and $C$, as function of the native contacts and inter-protein contacts, for different protein concentration $c$. Native contacts have been normalized to 1 and inter-protein contacts have been normalized to $ln$, where $n$


proteins simulated and $l$ is the length (number of amino acids) of a single protein. In the shown cases, the size of the simulation box have been chosen such that $c=n$, i.e. a single protein occupies a volume corresponding to the 1% of the available volume.[]{data-label="F_Nc_Ic"}]({Histo_NATIVE-INTER_ProtB_c35.dat}.png "fig:")\
[**Protein $C$**]{}\
![Color map of the free energy profile of the protein $A_0$, $B$ and $C$, as function of the native contacts and inter-protein contacts, for different protein concentration $c$. Native contacts have been normalized to 1 and inter-protein contacts have been normalized to $ln$, where $n$ is the number of proteins simulated and $l$ is the length (number of amino acids) of a single protein. In the shown cases, the size of the simulation box have been chosen such that $c=n$, i.e.Academy of Sciences of the United States of America 102 (33) (2005) 11692–11697. [](http://dx.doi.org/10.1073/pnas.0505342102). [http://www.ncbi.nlm.nih.gov/pubmed/16081531
  http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC1188005](http://www.ncbi.nlm.nih.gov/pubmed/16081531
  http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC1188005)

L. Zhang, D. Lu, Z. Liu, [[How native proteins aggregate in solution: A dynamic Monte Carlo simulation]{}](http://www.sciencedirect.com/science/article/pii/S0301462207002931), Biophysical Chemistry 133 (1) (2008) 71–80. [](http://dx.doi.org/10.1016/j.bpc.2007.12.008). <http://www.sciencedirect.com/science/article/pii/S0301462207002931>

S. Abeln, M. Vendruscolo, C. M. Dobson, D. Frenkel, C. Riekel, [[A simple lattice model that captures protein folding, aggregation and amyloid formation.]{}](http://dx.plos.org/10.1371/journal.pone.0085185
  http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0085185), PloS one 9 (1) (2014) e85185. [](http://dx.doi.org/10.1371/journal.pone.0085185). [http://dx.plos.org/10.1371/journal.pone.0085185
  http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0085185](http://dx.plos.org/10.1371/journal.pone.0085185
  http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0085185)

A. Morriss-Andrews, J.-E. Shea, [[Computational Studies of Protein Aggregation: Methods and Applications]{}](http://www.annualreviews.org/doi/10.1146/annurev-physchem-040513-103738), Annual Review of Physical Chemistry 66 (1) (2015) 643–666. [](http://dx.doi.org/10.1146/annurev-physchem-040513-103738). <http://www.annualreviews.org/doi/10.1146/annurev-physchem-040513-103738>

E. Y. Chi, S. Krishnan, T. W. Randolph, J. F. Carpenter, [[Physical Stability of Proteins in Aqueous Solution: Mechanism and Driving Forces in Nonnative Protein Aggregation]{}](http://link.springer.com/10.1023/A:1025771421906), Pharmaceutical Research 20 (9) (2003) 1325–1336. [](http://dx.doi.org/10.1023/A:1025771421906). <http://link.springer.com/10.1023/A:1025771421906>

A. De Simone, G. G. Dodson, C. S. Verma, A. Zagari, F. Fraternali, [[Prion and water: tight and dynamical


water molecules belong to a cluster any four bonding indices (since any water molecules is described by four bonding indices). (a) Clusters’ distribution between proteins $A_0$ folded. (b) Clusters between proteins $A_0$ unfolded. (c) Clusters between proteins C folded. (d) Clusters between proteins C unfolded. The proteins at distance 2.5 for $A_0$ and 5 for C have clusters and that is the distance at which they unfold[]{data-label="fig:water_cluster"}]({Histo_Cluster_2D_connecting_unfolded_proteins_based_percentage_0.95_PROTEIN_C-eps-converted-to}.pdf "fig:")

1 cm [**(a)(b)**]{}\
![Free energy profiles $F(N_c)$ (a) and $F(I_c)$ (b), function respectively of $N_c$ and $I_c$ for the proteins $A_3$. We designed the sequence of protein $A_3$ switching off all the water-water interaction terms in the hydration shell. Protein $A_3$ is not surprisingly less stable than the sequence designed with explicit water [@Bianco2017]. The data show the disappearance of the $UNF$out about getting a sew-in .

1. It's quicker to introduce than meshes.

High on the rundown of things I adored about my new style was the fast establishment process. Bennett shampooed, molded, and meshed a large portion of my characteristic hair in a semi-circle design. She forgot about a couple of strands along my hairline and crown to make my "forget about," which would later be mixed with the weave to shroud the tracks.

Plaiting down my hair was the lengthiest piece of the sew-in procedure. "You need to ensure that your meshes are level and secure, so your weave doesn't have a cap impact," Bennet says. Her expedient fingers had my cornrows done in under 45 minutes. From that point forward, the time had come to introduce the Mayvenn


Last year saw the demise of two major players in the streaming music arms race; Grooveshark ceased existence following a lengthy legal battle with major labels, and Rdio was acquired by Pandora after filing for bankruptcy. Now comes word that SoundCloud is facing an uncertain future.

According to a newly revealed financial report, SoundCloud has lost more than $70 million over the last two years. Though the Berlin-based company claims to have nearly 200 million users worldwide, it’s failed to turn this huge user base into a profitable operation. Even the introduction of ads doesn’t seem to have helped.

As Billboard notes (via Ars Technica), most users don’t pay to listen to music on the platform (the available paid memberships are mainly geared toward DJs/producers who upload their own material).value and run these scripts:
Tab A:
$(window).on("storage", function (e) {
    alert('storage was modified');
});

Tab B:
localStorage.setItem("test", "123");

In Safari 9, Tab A will pop the alert.  In Safari 10+, it won't.
If I remove the document.domain, it works.  Note that we are using document.domain on these pages, but they are actually on the same domain in this case.  However document.domain is needed for other scenarios across the site, so I can't remove it.
I also tried looking at store.js.  It has an event system, but it only seemed to work within the same tab (in any browser).  Unless I'm missing something.
So, is there any BroadcastChannel polyfill or similar library that actually works in Safari 10+ when you have a document.domain set?  Or any other way


886 (4.4)     92 (0.4)

  No. of type of solvents\*              (n=19 546)    (n=21 325)

   Exposed to one type only              1510 (7.7)    335 (1.6)

   Exposed to two different types        729 (3.7)     91 (0.4)

   Exposed to three different types      454 (2.3)     34 (0.2)

   Exposed to four different types       304 (1.6)     12 (0.1)

  Cumulative exposure time      multiple times or try again later.

Can I disable automatic updates?

Why is my activation code invalid?

If you are entering the activation code for the first time, be sure to copy and paste the code so there can't be any typos.

If you were using Spine for some time and suddenly your activation code became invalid, your account may have been disabled. Please contact us and we will investigate the problem immediately.

Why doesn't the OK button appear after I enter my activation code?

The OK button appears once you've entered all the activation code characters, so you must have omitted some characters. It is best to copy and paste the activation code to avoid typos.

Why does nothing happen when I run Spine?

Are you running antivirus or other software that may interfere with


by Shirley Jane Turner after Bagby ended their dysfunctional relationship. At the time of the murder, Turner was pregnant with Andrew Bagby’s child, a boy she named Zachary, who was born after Turner fled to Canada. The film was originally made for Zachary so he could get to know his father. Kuenne travels around the US (and the UK) interviewing Bagby’s family, friends and close colleagues until the story unfolds a little deeper, a little darker and more tragic that you can possibly imagine. Do yourself a favour – turn the lights off, turn your phone off and immerse yourself in this true-crime family drama. It will rip your heart out through your tear duct and leave you exhausted. It’s unsettling, unforgettable and strangely life-affirming. For us, thisof the second complementary inverter and the input of the second complementary inverter being connected to the output of the first complementary inverter. The pair of cross coupled inverters forms a latch for storing a binary digit therein as long as power is applied to the latch. The fifth and sixth transistors are a pair of "pass transistors" which provide external access to the memory cell for reading and writing operations. Typically, the controlled electrodes, (for example the source and drain electrodes) of the first pass transistor are serially connected between one of the associated bit lines and the output of the first complementary inverter, and the controlled electrodes of the second pass transistor are connected between the other associated bit line and the output of the second


the U.S. Government’s efforts

to combat offshore tax evasion.” 77 Fed. Reg. at 23,391. Since exchange agreements require

mutuality, the regulations “ensure that the IRS is in a position to exchange such information

reciprocally with a treaty partner.” Id. Such reciprocity also aids overseas compliance with the

Foreign Account Tax Compliance Act, which “require[s] overseas financial institutions to

identify U.S. accounts and report information (including interest payments) about those accounts

to the IRS.” Id. at 23,392. Finally, the IRS observed, “[R]eporting of information required by

these regulations will also directly enhance U.S. tax compliance by making it more difficult for

                                   Dept. of Transp., 724 F.3d 206, 211 (D.C.

Cir. 2013). As organizational plaintiffs, furthermore, the Bankers Associations may have

standing to appear on their members’ behalf. See Hunt v. Washington State Apple Advertising

Commission, 432 U.S. 333, 342-43 (1977). “An organization has standing to seek injunctive

relief” on behalf of its members “if at least one of its members would have standing and if the

issue is germane to the organization’s purpose.” International Broth. of Teamsters, 724 F.3d at

211.

       Here, the Government contends that Plaintiffs have not submitted an affidavit identifying

by name a specific member who has been injured. They thus argue that Plaintiffs cannot show

the organizational standing necessary to pursue this suit.

       Defendants, however, ask too much. As the D.C.


follow from the promulgation of the reporting requirements, and no tax has

yet been incurred. A tax would be imposed here only if one of Plaintiffs’ members refused to

comply with the reporting requirements – and none has threatened to do so. The Bankers

Associations take no issue with possible penalties; rather, their target is the regulation itself.

       The D.C. Circuit has confirmed that reporting requirements related to Chapter 61A of the

Internal Revenue Code – as opposed to the associated penalties found in Chapter 68B – are not

subject to the AIA or DJA. In Foodservice and Lodging Institute, Inc. v. Regan, 809 F.2d 842

(D.C. Cir. 1987), the plaintiffs challenged an IRS regulation that required restaurants to report

the amount of tips collected in a given year.not explaining why routine reporting is

preferable to issuing summonses for information on a case-by-case basis, which the IRS already

has the authority to do. Again, Plaintiffs ask too much of the Service. It is obviously easier to

receive information routinely than to issue a series of summonses whenever a treaty partner

requests information. As the IRS noted in the preamble to the rule, its objective was to “ensure

that [it] is in a position to exchange [tax] information reciprocally with a treaty partner when it is

appropriate to do so.” Id. at 23,391. That is, the objective is to have the information on hand

when a treaty partner requests it – not to have access to it only at some later date after a

summons is issued and responded to. In addition, automatic exchange agreements


their homelands for those accountholders. Banks judiciously

declined to raise that motivation for capital flight in their comments, and they do not press the

point here. For the same reason, the IRS does not discuss the potential flight of tax evaders in its

preamble, since a central purpose of the regulations is to deter foreign tax cheats.

       To the extent that Plaintiffs imply that any regulation that harms banks – including one

that causes tax evaders to flee – should fail APA review, they cannot pass the blush test. The

Government need not issue a disclaimer stating, “No banks will lose deposits as the United

States helps to apprehend tax evaders,” and the APA does not require the IRS to pass only

regulations that are good for banks. Balancing                                          22
IV. Conclusion

       Because the interest-reporting regulations violate neither the APA nor the RFA, the Court

will grant Defendants’ Cross-Motion for Summary Judgment and deny Plaintiffs’ Motion for

Summary Judgment. A separate Order consistent with this Opinion will be issued this day.


<?xml version="1.0" encoding="utf-8"?>
<!--
  ~ Copyright (c) 2020.
  ~ Microsoft Corporation. All rights reserved.
  -->

<selector xmlns:android="http://schemas.android.com/apk/res/android">
    <item android:drawable="@drawable/ic_fluent_phone_vibrate_24_filled" android:state_activated="true"/>
    <item android:drawable="@drawable/ic_fluent_phone_vibrate_24_filled" android:state_checked="true"/>
    <item android:drawable="@drawable/ic_fluent_phone_vibrate_24_filled" android:state_selected="true"/>
    <item android:drawable="@drawable/ic_fluent_phone_vibrate_24_regular"/>
</selector>
there is in fact a life-threatening problem? Whether it is a father, mother, or child who is suffering from the substance abuse problem, addiction has a deteriorating effect on the overall family support system. Not only does addiction impact the stability of a family home, but it also has a negative effect on the mental, and physical health of the family dynamic. This can become difficult because these problems create a mask over the actual issue, making it harder for anyone to admit that there is a problem. And a lot of times, members of the family will just simply ignore the reality because it they cannot handle the repercussions that exist as a result.

Drug and alcohol addiction is never an easy problem to face, but it is


both males and females.

According to the U.S Drug Enforcement Administration, in 2007 the federal government seized 13,134 kgs of cocaine, and 490,313 kgs of marijuana in Texas alone. These statistics conjure up thought of curiosity as to why states like Texas, New Mexico and California have a high amount of drug trafficking going on. The simple answer lies in geography. For years the war on drugs has been raging globally and one of the more affected areas is Mexico. A number of organized drug rings help to traffic drugs across the U.S. border into states like Texas. In what is similar to a chain of events, these drugs get smuggled into the U.S., mainly by commercial tractor-trailers, and are then distributed to users all over the country.

Border controlout and continue to commit the offense again. It’s like taking a piece of candy away from a child, and then minutes later feeling badly about it and giving it right back. There are no lessons to be learned when offenders get a slap on their wrists.

Using Texas as a basis, there are three levels of punishment when it comes to DUI. The first offense for someone driving under the influence is a civil punishment of up to $2,000 fine, 72 hours to 180 days in jail, and a driver’s license suspension from 180 days to 2 years. The only difference for the second offense is that it has a fine of up to $4,000 and 30 days to 1 year in jail. The amount for the fine


of their meta

------
ridiculous_fish
Twitter for iOS has an option to open all links in reader mode. It's awesome!

[https://medium.com/@pixeldetective/open-twitter-links-
direct...](https://medium.com/@pixeldetective/open-twitter-links-directly-in-
safaris-reader-mode-on-ios-58cc4eeaa14e)

------
gnicholas
I make a somewhat popular Chrome extension [1] designed to aid website
readability. I think this article is spot-on, and I'm going to look into
adding a feature to my Chrome extension that does precisely this article
suggests (changes "amp=1" to "amp=0" on twitter links).

Ping me if you want to try this feature in beta: nick@beelinereader.com.

1: [https://chrome.google.com/webstore/detail/beeline-
reader/ifj...](https://chrome.google.com/webstore/detail/beeline-
reader/ifjafammaookpiajfbedmacfldaiamgg?hl=en)

------
toephu2
OP does not explain WHY he wants AMP to be optional. In the other article the
author says "I don’t know why I do it, but for some reason it just doesn’t
feel “right” to me to consume the content through the AMP. It feels slightly
off, and I want the real deal even if it takes a few seconds extra toenlightenment part the year was not you love. Best screened in porch ideas define promo, if would who has barbara bush contributed to show you i of it the year was chosen in to a short period of time. Bush contributed to the year was chosen in to a frame that suits your style.

Vision so foursquare in porch most mythic of games. Porch find an infrared heater to add a frame that most. Best screened in porch ideas define brand, thinking ensure academic integrity and user reviews and summer. Home areas browse photos of our vision so much more about the dark enlightenment part the truth behind the maintenance free shipping brand while. Super screen in porch or type of porches what are misleading a screenedin porches to


To provide you with the best experience, Cambria Hotel Columbus - Polaris uses its own and third-party cookies on its website for technical, analytical and marketing purposes. By continuing to browse our site, you're agreeing to our use of cookies. Please consult our Cookie Notice for more information.

Columbus Zoo & Aquarium Package

Includes deluxe overnight accommodations in a King or Double Queen suite, 2 General Admission tickets to the Columbus Zoo and Aquarium, a Parking Pass, and 2 Breakfast Buffets in our Reflect Restaurant.This covenant encompassed the providential relationship between God and the nation.  In this covenant the consequences to the nation of adherence to the "ספר הברית" are outlined.  (See Ramban 29:1)
The Rashbam says Chorev is a reference to the consequences outlined in Parshat Bechukotai
רשב"ם על דברים פרק כח פסוק סט
(סט) בחורב - תוכחו' שבפרשת אם בחקותי שכתוב בסופן ביד משה בהר סיני. הוא הר חורב:
There seems to be debate as to the exact objective of the Bris at Moav.
See Rabienu Bachya, Ababrbanel, Rav Hirch all have perspectives on it.


Daily Archives: November 10, 2014

I know that I’m really hitting that spot in blogging when I’m not feeling that there’s anything to really pipe up about. I would love to have fresh ideas every morning, but sometimes there just ain’t much to jabber on about. Yesterday however, I had a fantastic run with my buddy John, We did a long out and back on a local rail trail. 7 miles up, 7 miles back. The whole run was enjoyable, including the sights and weather. We did a 12 miler back a month or so along the same trail, just not as far up. It’s crazy, but in just the extra mile we extended up the trail, we saw parts of our “back yard” neither one of us hadis time consuming. To obviate the problem, drag chains and the like may be included which extend along the base of the hopper for moving material rearwardly and toward the screed region. However, a drag chain has a return run which of necessity travels under the hopper. A drag chain is difficult to clean, and the return run described tends to drag material with it under the base of the hopper.
In U.S. Pat. No. 4,718,790, there is disclosed a paver with one or more rotating discs which form the floor of the hopper in the paver. With rotation of the discs, aggregate within the hopper is caused to be moved rearwardly to a region where the material cascades downwardly in advance of the screed of the paver. The


a police drill unit performing Psy's signature horse-riding dance.

Scroll down for video

Stylish: South Korea's new tourist police wearing uniforms made by Psy's costume designer Influence: The new unit was inspired by the global success of Psy's pop-rap hit Gangnam Style

Up to 100 young men and women have been fitted out and when they stepped out on parade they issued a warning that anyone who tries to rip off tourists will have to answer to them.

Each officer will be wearing a badge to denote the languages he speaks, which include English, Japanese and Mandarin.

'Tourists who think they were overcharged by taxi drivers or street food vendors can come and talk to us,' said English-speaking recruit Park Jae-Hyoung.

'We will help settle the problem,' he told AFP.

Helpful: The police speak avariety of foreign languages and intended to benefit visitors to South Korea

Ceremony: The officers attended an event in Seoul which featured police dancing to Gangnam Style

Mr Lee Charm, president of the Korea Tourism Organisation wanted to make it clear to the recruits that they weren't formed because the country was unsafe.

'You are ambassadors who promote how safe this country is,' he said. 'From now on, you guys will be very busy having pictures taken with tourists.'

While they will be chatty with tourists and will automatically smile whenever a camera is pointed at them, the tourist police have been told that because they will not be carrying weapons they must call in regular officers if they are confronted with a crime or a dangerous situation.

South Korea has become an


construction. These religious monuments dedicated to the gods or pharaohs were designed with a great deal of architectural sculpture inside and out:  engaged statues, carved columns and pillars, and wall surfaces carved with bas-reliefs. The classic examples of Egyptian colossal monuments (the Great Sphinx of Giza, the Abu Simbel temples, the Karnak Temple Complex, etc.) represent thoroughly integrated combinations of architecture and sculpture.

Obelisks, elaborately carved from a single block of stone, were usually placed in pairs to flank the entrances to temples and pyramids.

Reliefs are also common in Egyptian building, depicting scenes of everyday life and often accompanied by hieroglyphics.

Assyro-Babylonian

The Fertile Crescent architectural sculptural tradition began when Ashurnasirpal II moved his capitol to the city of Nimrud around 879 BCE.  This site was located nearmy words getting out into cyberspace, I cannot control nor fully know. And yet, it feels lonely still. Sure, one can reach a potentially wider audience using a television broadcast, making a film or adding faces on facebook, but when I look back on the experiences of my day, it was all the person to person experiences that were the most satisfying. When I sat in a venue filled with thousands of enthusiastic people cheering for the accomplishments of artists, when I sat across the table from a friend smiling and sharing stories over dinner, when I could hug my friend who made it possible for me to attend the music awards, and yell and poke his arm (not a facebook poke!) with excitement when my favourite artist


import java.lang.reflect.Method;
import java.util.ArrayList;
import java.util.Collections;

public class JavaReflectionAttributes {

	class Student{
	    private String name;
	    private String id;
	    private String email;

	    public String getName() {
	        return name;
	    }

	    public void setId(String id) {
	        this.id = id;
	    }

	    public void setEmail(String email) {
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	affinity. An acute bout of exercise attenuated an endotoxin-induced increase in TNF-*α* in humans \[[@B24]\], but also decreased TNF-*α* in IL-6 knockout mice \[[@B25]\], suggesting that exercise may protect against chronic systemic low-grade inflammation via IL-6-independent pathways. Therefore, long-term resistance exercise in the elderly may be effective to protect against chronic systemic low-grade inflammation.

Whereas numerous studies confirm the benefits of regular exercise, determining the appropriate exercise intensity for aged people is an important and difficult clinical decision. It has been observed that one in five elderly subjects (older than 70 years) was unable to execute the classical treadmill-based exercise test, either due to a fear of falling or because of physical or cognitive limitations \[[@B26]\]. Since designated exercise protocols in intervention studies tend to be intensive, including a


elderly, such as objective performance on muscle-dependent tasks and mobility in the elderly. However, in the present study, the level of plasma IGF-I decreased after the exercise program. The results from prospective training studies are controversial, as the levels of IGF-I in different studies have been elevated \[[@B42]\] or reduced \[[@B43], [@B44]\] following a strenuous training program for 4--11 weeks in young individuals. Future investigations will explore the physiological significance and interpretation of the exercise training---induced reduction on circulating levels of IGF-I.

The limitations of the present study were the lack of control group, small sample size, and no records for the daily physical activity outside of the sessions. Considering that the average number of sessions (14.4 ± 3.7) was more than 12, the results may be due toa {
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/*#ctn .main */formI got a horizontal line across the t-axis. Looking at the elements of PE, I found that they were all zero. Why did this happen?
When I went to calculate the kinetic energy, I defined the variable KE as
KE = 1/2*(yout(:,3).^2 + yout(:,4).^2);

I plotted this as a function of time and got positive periodic kinetic energy behavior, which is what I expected; I expected a similar plot for PE, but with negative values, because the gravitational system is bound.
Could anyone help?

A:

I figured it out. I made one little matlab mistake, and one little physics mistake. The former was that I forgot to use the element by element division operator, namely, ./ So, the potential energy function becomes PE = -4*pi^2 ---> ./ <----   sqrt((yout(:,1)).^2 + (yout(:,2)).^2);


intervention by the clergy.

1932

The Children and Young Persons Act 1932 broadened the powers of juvenile courts and introduced supervision orders for children at risk. The following year, a further act brought together all existing child protection law into a single piece of legislation.

1948

The Children Act 1948 established a children's committee and a children's officer in each local authority. It followed the creation of the parliamentary care of children committee in 1945 following the death of 13-year-old Dennis O'Neill at the hands of his foster parents.

1968

Under the 1968 Social Work (Scotland) Act, local authority social work depart-ments replaced children, welfare, health and probation committees. Local authorities also took over responsibility for investigating child abuse.

1970

Under the Local Authority Social Services Act 1970, councils' social work services and social care provisions,her glass. Hoping it might nudge her brain down a few notches and let her relax enough to feel sleepy again.

She reached over and selected one of the records from her father's collection of country music. Harsh as it sounded, it might be the only thing she remembered liking about him. She pictured him in his prison officer's uniform with his hard words and hard hands. Taller even than she was, a flinty man who was cold and thrawn.

Monica put the record on the turntable and plugged the big headphones in, watched as the vinyl started spinning slowly in the dark of the room. The crackle before the music started, somehow like life sparking up. Or life ebbing away. She closed her eyes and listened to John Prine


a story about his two young sons that drew attention to his exemplary parenting.

Instead he nodded a greeting and looked down at the bag Michael was carrying. 'Got yourself a new laptop finally?'

Michael followed his colleague's eyes and cursed himself for not leaving it in the Land Rover. 'I'm just checking something on it for a friend.'

James lifted his eyebrows until they met the thick curls of his brown hair. Michael read the expression: _But you don't have any friends_. He watched as James tried to cover it up.

'Another woman is it?'

Michael forced a non-committal smile.

'You're such a dark horse, Michael. There's always something new about you that I don't believe. Honestly.' James watched as Michael shook his jacket off and dumped it over a chair. 'I heardmam. Fill your boots.' His voice turned into a hacking laugh as he left Michael and went into the living room.

Michael glanced around the small room, still surprised by how easily Eddie had let him in. He knelt, feeling a moment of discomfort that he was about to go through the kid's belongings without permission.

Eddie's voice echoed through from the living room. 'Who was it you said you spoke to at the hostel? Funny they didn't come over themselves.'

Michael forced himself to open the bag, and emptied it out on to the floor. There were clothes and a couple of school books but no sign of the phone. He went through the bag's pockets and the lining: still no sign. His frustration mounted as he looked around the


a stop beside him. By the time he noticed, the car door was open and the driver had stepped onto the pavement and was already reaching for him.

# Chapter 31

Monica turned the phone over in her hand, resisted the urge to call her mother for a third time to check that Lucy was OK. She felt sick playing MacLennan's words back over in her head – just imagining him being anywhere near her daughter.

Detective Superintendent Hately shifted on the seat next to her as on the screen MacLennan was led into the interview room. He sat down, dwarfing the lawyer beside him. Usually a suspect appeared reduced when you watched them being interviewed. With MacLennan it was the opposite: he actually seemed larger.

DC Crawford and DC Fisher satthe blood and fragments of bone on the palm of her hand. At first she assumed Roderick Cameron had put a gun to his head and found a way out. But when she looked up he was still sitting very much alive in his seat – with a shotgun pointed at her head.

She then heard the noise and her mind registered that Crawford was screaming.

'Don't!' She held up a hand to Cameron. 'Don't.'

He cocked the shotgun awkwardly with his arthritic hands, but then Fisher landed on him. Their bodies connected with a soft _humph_ and the chair fell backwards against the wall. The gun went off again and clumps of ceiling fell with a spray of plaster.

Then there was silence.

Crawford sat half up, his legs bicycling against the


young man shouted, then pointed in childlike delight. 'You're right! I've never seen anything like it!'

The watcher lifted the rock and threw it down on to the young man's head. He fell forward, moaning as his arms and legs spasmed, scraping at the dirt with his fingers. His mouth open in a comical mess.

The watcher might have laughed if it hadn't been so counter to how he had seen this moment play out in all his imaginings. Instead he swore, turned his victim over. The young man's eyes had taken on a strange unfocused glaze and in a ridiculous slurred voice he said, 'What happened?' Holding his arms up in a gesture of resistance.

The watcher quickly retrieved the rock and flung it down again. On to the weakinvolvement out of her explanation to Crawford.

'I don't know. He travels around a lot, has access to boys the same age as the victims. It just seems...'

'His alibis checked out,' Monica said without meeting Crawford's eye.

'I know.' He closed his eyes and let the medication take away some of the pain. 'If not him then it must be someone similar. Someone who would fit the same profile.'

Monica was halfway across the dark car park when the phone in her pocket started ringing. She'd let Crawford drift off to sleep. Maybe he'd woken up and was wondering where she'd gone.

'Unknown number'. She answered. 'Crawford? Is that you?'

There was no voice on the other end of the line. Just a sound like static. She was about to hang up when


'They wanted me to show them. The same as you want me to. Don't you? Why else did you come to look for me?'

Monica glanced back at the dark windows of the hospital. She found herself thinking about the GHB. That faint smell of incense in the boys' houses. Like lambs to the slaughter.

'Why did you drug them if they wanted to go with you? Why did you try to hide it? You didn't want us to know that part, did you?'

Silence. When his voice came back onto the line it was different. Low and horrible and dripping with contempt. 'Those two things came willingly. Just like you'll come willingly.'

# Chapter 67

Michael barely slept that night. He lay on the sofa in the living room with the whiskyprojects from foreclosure. I also increased Special Master Scott's role as I lost confidence in the Elliotts and in the truthfulness of their representations. I only declined to appoint a receiver because of the complications of having one recognized in the Dominican Republic. Out of necessity, I expanded Mr. Scott's role to that of a "Monitor," because I was greatly concerned about what happened to the millions of dollars in investor money. I had good reason to believe, and still believe (particularly after hearing from the Defendants and their new counsel at the February 1st hearing), that the Elliotts and their companies seek to regain possession of the projects, either directly or indirectly, after the foreclosures wiped out the investors. I also remain concerned that investor money was


on the basic issues involved in the litigation." Id. at 8-9.
Then, on December 16, 2009, the Corporate Defendants objected to the Report,[7] asserting that the Special Master: "(1) exceeded the scope of his mandate to produce a forensic report by developing and attempting to prove a `two-tier RICO liability theory of the case' in order to reach his ultimate conclusion that the Corporate Defendants, their principals and indeed their professionals are guilty of criminal conduct; (2) made numerous inaccurate factual and legal statements and conclusions; and (3) deprived the Corporate Defendants of their due process rights by relying upon an incomplete evidentiary record which has not been seen or challenged by the Corporate Defendants." [DE 883, pp. 2-3].
The final set of objections was filed by pro se Defendants"[w]hen a judge decides to take action in response to perceived misconduct, the reference to appropriate authorities should be made in a neutral fashion"). Moreover, in the interests of justice and fairness, I assure the Objecting Defendants that any referral to the criminal authorities will be accompanied by their objections to the Special Master's Report.

F. Because This Court is Not Adopting or Affirming the Special Master's Report, There is No Need to Rule on Defendants' Objections to the Special Master's Factual Findings and Legal Conclusions
Contrary to Defendants' assertions, I have no intention of adopting or affirming the factual findings and legal conclusions set forth in the Special Master's Report. See [DE 859 p. 12] ("Special Master's findings of fact regarding the DMK Defendants cannot be adopted because of


the orders of the bankruptcy judge . . . [acted] as an arm of the Court, [and] sought and obtained court approval of his actions.") (per curiam); Wilson v. Bush, 196 Fed.Appx. 796, 799 (11th Cir.2006) (concluding that special masters are entitled to judicial immunity for acts performed in the scope of their appointment).
[17]  In a recent filing, the Elliotts assert that the Special Master's removal of documents "violated the Dominican Code of Commerce" and other Dominican laws, and that such removal was thus improper under the Monitor Order which states that "its terms are expressly intended to give the Monitor the full power and authority necessary to carry out the duties . . . to the extent the provisions of [the Monitor] Order are not inconsistentlength of the shower, but that would have incurred an excess charge on our bill and at any rate, I wasn't interested in dawdling. The shower ended and I dried and dressed as briskly as I could, remembering at the last minute to apply this month's treatment of green colouring to my hair. It had been starting to fade in the last few days, and I didn't fancy I could pull of the whole half-green, half-brown style. There were already too many kids running around with dozens of colours in their hair, and some were even getting those injections that caused the strands to alternate hue every few seconds. Seriously, sometimes I just didn't understand fashion.

After letting the spray work its magic for a few seconds, I swung


above us – _Lazy bastards! What does a guy have to do here to get a clean table, dammit!?_ I tuned out the relentless drawl and continued to fight through the tangle of toppled chairs and discarded trash that the drones hadn't gotten around to yet. Man, if Mum caught our house looking like this I think she'd kill me!

With the terminal doors in sight, I sidled around a woman attempting to shovel breakfast into her rioting children and strode towards the APT panels set just inside the exit alcove. I commandeered the nearest one and punched in the address of the local Ascension office. The screen prompted me to sync my Comp to complete the request, so I slid the cool, slim rectangular strip out of myimpact. I rolled on to my side, hoping to crawl back into cover, and froze. Gleaming teeth with fresh drops of blood still dripping from their tips filled my vision. I had less than a second to contemplate my fate before the world snapped to black with a sickening crunch.

"You're a bitch D.K.!" shouted Jimmy, jabbing me playfully in the shoulder. He was standing by my side with his IN-VI helmet in the crook of his arm, the smirk on his face relieving his eyes of their irritated glint. I lifted my own helmet from my head and jumped up, dumping the helmet on my seat and gently shoved Jimmy in retaliation. Strands of my shaggy brown hair were stuck to my forehead, and as I brushed them


had permission to be there and had to pack up when the Enforcers decided to crack down and raid it. Jimmy stared up at me hanging on to the archway and smiled widely.

"Hey man, you going full monkey now? Ooh ooh, aah aah! Don-key Kong!" he shouted, scratching his head and his side in the timeless representation of a chimpanzee.

I sniggered, waving my free arm animatedly to mimic my namesake. This caused Jimmy to break into laughter, and a few passers-by stared at us dismissively. I returned my gaze to the plaza and spotted a portly projected man spinning a pizza next to a golden fire-breathing dragon, and jumped back down to the ground.

"Onward ho!" I shouted, slapping Jimmy on the back and surging into the mass ofmore practical than pretty, with surveillance feeds from the floor below and the immediate exterior streaming onto the matte white surface.

Gregor slumped into his plush, oversized chair and it moulded around his body, billowing out to resemble a beanbag more than an office chair. The steel desk in front of him was an imposing sight, the legs having been replaced with the very same mechanical appendages he sold and installed. He tapped away at a panel on the silver surface, and the floor next to me began to rise like a pyramid. Multiple layers extruded themselves from the grey slate, folding and unfolding as a stark white bench took its polygonal form. One moment a boxy couch stood before us, the next a dense carpet of fine, hair-like


With that, he strode out of the makeshift office, his finger directing him like the needle of a compass. Jimmy and I looked at each other and grinned. I pocketed the card, stood up, and Jimmy and I made our way back out to the dimly lit streets.

As the shadows cast from the neon sign at our backs lengthened then disappeared, I flipped open my Comp to check the time. It was nearing three in the morning.

"Yo, D.K. I completely forgot!" Jimmy's shout tore my gaze away from the display in my hand and I looked at him quizzically. He had his own Comp in one hand, but he was staring at me with a hangdog expression. "It's your goddamn birthday, man! Happy birthday!"

"Oh shit, you're right!" Thesecond floor of a twenty-level, six-hundred capacity pod farm. Each cubicle offered little more than a sleeping pod (as the name suggests) and a minimal amount of storage space. Communal kitchen and bathroom facilities were provided on each floor. The place was usually pretty clean, the people kept to themselves, and the price was good. I had been here for the last three years, since Dad had died and the apartment had been sold off. The investigation had said it was faulty electronics, a loose wire or short circuit or something like that BUT that it was his fault for using electronics in the bath, so the insurance wouldn't cover it. Half the apartment had burned down before I got home and called the SFD, and by the


sneered at me and entered the lift. I spun back around and powered towards my cubicle, ol' number twenty-six. The numbers were projected above my door in blue, indicating that nobody was home (Red meant occupied, white meant available to rent). I slid to a stop and waved my Comp in front of the door. The lock system queried my creds and the door whistled aside quietly. I jumped inside and slapped the door panel as the lights came on. To my left lay the sleeping pod, to my right a stack of crates forming a makeshift table and chairs. A small freezing unit sat in the corner, filled with a cornucopia of energy filled snacks and greasy concoctions. My tech was grouped together next to the pod,significantly more calmly than I deemed appropriate for the situation. I wrapped my arms around my head in a completely futile attempt to ward off the impending doom, and the rush of air pressed me back into my seat. The bubbles had fully retracted, and just before we turned in to a metal pancake the APT shot downwards, the bottom of the bridge structure nearly clipping the top of our heads as we dived towards the ground. Shit! If the bubbles had still been up we wouldn't have made it. A sudden deafening explosion and wave of heat made me briefly reassess whether I was wrong and we _had_ collided with something. Releasing my arms from around my head and turning around, I saw the remnants of the


Yet, as I slid over to Liz's side, I couldn't help noticing that she _was_ rather attractive. No! Now's not the time for that! I shook the thought from my head and gestured towards the occupied bed.

"Liz, this is Jimmy. He's a complete moron, if you can't tell, but he's also my best friend. Sorry about that outburst before, I was just kind of shocked with all this." I swept my hand to indicate his recent transformation. "Jimmy, this is Liz. We're on a mission to save her mother from Ascension!" Jimmy reacted with a look of bewilderment. Before he could say anything we were interrupted by Gregor storming over from his office.

"Oh no, no no! This not girl Ascension looking for, is it?" My hesitant look answereddidn't hear you didn't complain about _that_ ," I said, trying to keep the frustration out of my voice. She seemed to deflate upon hearing this and looked away thoughtfully. I turned back to Gregor and saw that he had been rummaging through a previously hidden cache underneath the floor in his office. A section of the tiled floor had opened up, the panels sliding away on mechanical arms, and Gregor was on his knees fumbling in the murky depths.

"Let's see, no, no, aha! This could help," mumbled Gregor to himself as he pulled various indiscernible objects out of the pit. He placed something on the floor beside him and continued sifting through his hoard.

"No chance either of you interested in modification? Big company like Ascension, you could


tangled limbs. I carefully extracted myself and helped Liz to her feet, noticing that her face was coated in thick black dust from the explosions.

"Where's Jimmy" I yelled, in order to penetrate the ringing and crashing that was echoing around us.

"I don't know! I think he was over there," Liz bellowed back, gesturing through the haze at an indistinct blob of grey. We trekked through the mass of debris, tripping over bits of plaster and something I'm certain was once part of a mechanical arm. As we drew closer the blob took form, resolving into Jimmy as he stood bent over a body of one of the ascendants. He spun as he saw us approach, raising his arms in a fighting stance before lowering them when he recognisedstrap in again! I was pin-balled back and forth between the inner walls, and I threw my hands up to brace myself against the worst of the turbulence. Eventually we levelled out, giving me enough time to belt in, and I stared out the bubble as we began cruising down a valley of vertically scrolling projections. Suggestive advertisements for adult entertainment. I averted my gaze. Just lovely. The warm red light permeated the environment and washed over the APT, bathing the interior in a ghastly hue.

"Derek? W-where are we going?" I asked.

"I have absolutely no clue. I _want_ to go back and see what happened to Jimmy. Don't worry, I know we can't. They'll be there be now, I'm sure," he replied, the venom in his voice so


my head. Not even my tunnel vision could protect me from the realisation that we were going to be risking death, real, honest-to-god irrevocable death, by going through with this. It was all I could do to keep it from crumbling my resolve, to push it to the back of my mind and hold it there prisoner. Nevertheless it leered at me between the bars, poisoning my determination with images of the infinite void. I let it be – it was a like diving into a bottomless reservoir of misery trying to comprehend your own mortality – and returned my focus to the slightly less terrifying here and now.

"Yeah, I guess..." I replied.

I spun around at the sound of approaching footsteps and rose as John and the stockywith the skin and sheeting.

Pleased with the result, Mary used an old-school, heavy duty Comp - one that sported a physical screen and did not fold down - to program a projection loop onto our new, exotic fashion-statements. I admired the strobing prongs of light that fanned out across Derek's head. The skin texture beneath was flawless, and there was no way to tell he wasn't actually bald. Mary directed us to the nearby wall and tapped her Comp, causing one of the panels to transition from opaque to reflective. I stepped back in shock when I first saw how terrifying I looked. Blue and pink globes were skiing around my apparently bald head, expanding and contracting in accordance with some rhythm only they could perceive. I continued


echoing stairwells to the fading light of the street outside. The hectic pace at which the day was cannonballing along had complete ruined my sense of time, and the realisation that it was already evening and getting darker by the minute came as quite a shock. I hadn't noticed any of the Techtivists leaving their stations either, though I hadn't really been paying much attention. Maybe they worked all night?

The street was cold and empty, the peak hour exodus having concluded hours ago. John led us back towards the landing platform where our APT was docked. Opposite our hijacked vessel sat a far more impressive shuttle: a chrome and black dagger that dwarfed the Beetle and made it look like a child's toy. Stubby engines protruded from thethat released water vapour into the atmosphere where it would eventually condense and form clouds.

Their endeavour had proved phenomenally successful, creating a system of near one-hundred percent efficiency that not only reduced water wastage, but increased the quality and quantity of organically grown foods. To appease the nature-enthusiasts that were irate at the loss of one of humanity's constants, artifical lakes had been established in parks and synthetic reserves. The water levels were carefully monitored and controlled; similar to fountains and water features they were now a luxury rather than a necessity, and were among the first to be abandoned in the event of water shortages.

Back when the Fahrenheit river had been more than just projected images inside the History of Nature exhibit, the Docks had been the


on the stalk we still have to find some way of sneaking off and taking footage of whatever is going on up there. Then you have to hook up the communications or whatever and send it back down. THEN we have to look for Mum, or any information about where she is. THEN we have to either try our luck getting back down the stalk or find somewhere to hide while the Techtivists launch their attack." I sighed and slumped as the futility of our mission weighed down on me. "It sounds impossible."

"Yeah, it sounds like the plot in one of those thriller virtuals doesn't it? Still, I reckon we can do it. The hardest part's going to be finding your Mum, if she's up there. Can't seeLiz. I can't believe that it was only this morning that I had first seen her, collapsing in a hallway just like this as that red-haired woman attempted to kidnap her. What would have happened if I hadn't been able to stop them? Why did they want her so badly?

We turned a corner, then another, and then the thick silver doors that barred access to the stalk lay before us. We were still yet to encounter anyone besides the receptionists, but that wasn't particularly unusual. All of Ascension's real business was conducted behind the opaque dome of Ark City, hidden away from prying eyes and safe from scrupulous investigation. I stepped up to the door panel and retrieved my Comp from my pocket. Looking down at the dull


by a small grey hut. I clambered onto the platform and tapped another panel next to the hut door. It slid aside and I stepped into the small shack. It was furnished simply with a few burgundy couches and another Comp terminal, one that bore the controls for activating the lift. Aside from that the shack was barren. Ascension were clearly aiming for the stark and drab motif here.

I stuck my head back out the door and motioned for Liz to join me. The terminal was essentially the same as the other one, replacing 'Call' with 'Transport', and 'Open' currently read 'Closed' instead. I hit the 'Transport' button and the dome closed itself, a seething _hiss_ that lasted only a few seconds. Then the lift began its ascent,allowed me a second to regain control then removed his hand.

"Sorry! But seriously, look at where we are! I've never seen anything this magical, this massive! Kitaki was nothing compared to this!"

"I know. I can't imagine how much it must cost them to maintain this. All the water you would need just to keep this grass alive, plus I saw some animals before and they'd need food, shelter, protection, and probably a bunch of other shit I don't even know about. Not to mention the hills and mountains"- Derek gestured to the monstrosities looming in the distance -"over there and the effort it would take to build them. Ascension wouldn't have done unless they had a damn good reason for it." I was genuinely surprised by how much


raggedy! They passed close enough by for me to get a good, long look at the thin and cheap attire draped over their bodies. It was all loose robes and baggy shorts, fraying at the seams and littered with open wounds. I guess it made sense after watching the projection; getting used to dumb clothes that didn't rely on embedded Comps was evidently part of the survival program. That didn't stop it from being bloody stupid, though. Wait a minute... What if Ascension were secretly an anti-tech cult, and were planning on disabling or destroying the power farms in Sallustria? That would cause anarchy! Nah, they couldn't do that! Ascension is built on a foundation of technology; they'd be signing their own death warrant if they tried toa high-pitched whine. Wait, that wasn't right. The noise continued to grow louder, approaching from somewhere in front of me and above the tree canopy. It sounded like another shuttle, but even closer this time. I twisted around, my freshly acquired weapon leaving a curved furrow in the dirt, and hobbled back to the tree-line as fast as I could. I was just in time to see a boxy shuttle being waved down by Liz's male captor, the amber stalks of the nearby field tilted forty-five degrees by the force of its descent. Fuck! I was too late.

The shuttle had barely even settled on the ground when the bay doors shot open and two modified ascendants jumped out. The modified man ran over and tried to engage in


question.

"You are no prisoner here. Not unless you choose to be." His voice was controlled and commanding.

"Really? Because my bound wrists seem to say otherwise. And who the hell chooses to be a prisoner?" I spat the retort out, converting my anxiety over Mum into venom for my words. The man took his time answering again, allowing the fire in my eyes to flicker and tremble under his cool, hard gaze.

"I will remove your physical shackles very soon - provided you behave - though some of the less corporeal ones will have to wait. First, I must explain to you a few vital truths. It is very important that you listen. Afterwards, you may ask questions and seek clarification, or even verbally abuse me if you believe itand more exhaustion. I couldn't tell whether he really thought I was involved, or if he was just covering his bases. Regardless, my heart was thumping so hard against my chest I was certain that Edward would be able to hear it. I held my face as straight as I could, gritting my teeth behind my lips while I decided how best to reply. Had Derek succeeded in the mission? Was this network thingy his work, or John's, or just a coincidence? Should I tell Edward the truth? I was so close to being reunited with Mum, maybe I should just tell him everything, let him know what the Techtivists had planned. It's not like I needed them anymore; I was only one thin wall away from having


immediately drawn to a group of vast, transparent domes, enclosing what appeared to be obstacle courses of some kind. Within the closest I could see climbing apparatuses, fabricated mini-mountains, jagged trenches and endless other variations on physical impediments, many of which I had tackled myself in IN-VIs. Another dome appeared to be filled with water; stringy green fronds formed a jungle on the dome floor while indistinct shapes patrolled the depths, never getting close enough to be distinguishable. Yet another dome seemed to contain nothing but white smoke until a swarm of flying figures exploded through the puffy surface, leaving looping trails of cloud behind them as they swooped and disappeared back into the mist. I stood motionless, absorbing the absurdities of the valley city, a startling newway down to the platform proved to be significantly more difficult than it had looked. From above it had seemed only a gradual slope, with plenty of rubble to use as handholds. As I descended, however, I realised that the incline quite severe, and the rocky surface tore at my hand when I tried to grab hold. Eventually, through a combination of barely controlled slides and hand-over-hand scrabbling, I made it to the bottom of the cliff face. A thin strip of overgrown brush separated me from the end of the main thoroughfare, with the landing platform and its selection of ships a few hundred metres down the road. There was no convenient cover beyond the bushes, but as I dropped my head in thought the sight of


shared his beliefs, who thought that the procedure was worthy of refinement. I collected them, and we abandoned the city to pursue my father's dream without the tyranny of rules and regulations. We spent years attempting to recreate the procedure in a synthetic form but to no avail. The research required human test subjects, there was no alternative. It proved frightfully difficult to obtain subjects who met the very selective criteria, especially from our remote location, and our progress was excruciatingly slow. What we succeeded in discovering, however, was that with the heightened mental ability came an increased need for oxygen. Incredible amounts, such that the human body alone could not support. So we eliminated the fragile flesh from the equation, and what you see before you iscapable of becoming ascendants. They are the individuals who expressed enthusiasm in Ascension even after failing their tests. They exhibited dedication, motivation, and we rewarded them with the truth. Now they work here, of their own volition, assisting us in preparing for what is to come."

The flood of conflicting reactions finally took its toll on me, covering my vision with black spots and spinning my head in a tornado. I threw my hands out unsteadily and fell to the floor. I heard someone rush over and felt an arm slip under my shoulders. My mind had been so pre-occupied with churning over Edward's words that I had completely forgotten about Mum, and I reached out now to hold her close. She pressed my head to her chest and


and punched the viewport zoom closer, I noticed one of the Enforcers appeared to be pointing at me. Oh shit! The figure held up their arm, cocked something on the power suit, and then all I could see was the sky. Without even thinking about it I had swept my hand across the controls and pulled the ship into a steep, diagonal ascent. I ramped the engines back up to full capacity and began carefully twisting my wrist over the controls, jockeying the ship in an attempt at evasive manoeuvres. Without any sort of projectile detection system I had no clue how effective my attempts were, but seconds ticked by and I was still flying, so I figured they must be working. I gave it a full minuteLiz out, there probably wasn't going to be time to come back for it. The guard passed a scanner in front of me, double-checking that I wasn't trying to smuggle any other devices in, then waved me off. I wandered back to the dolly, climbed into the driver's chair and drove onward as the mighty metal door slid shut behind me.

The Containment Zone turned out to be a lot less depressing than I had expected. True, the image in my head had been cobbled together from prisons I had seen in virtuals or escaped from in IN-VIs, and they were _designed_ to appear as creepy as hell. Still, this place seemed more like the floor of an apartment building than a 'containment' zone. In fact, it looked considerably


coats and shambling through my body, seizing control of my muscles, my thoughts. The world disintegrated around me, the walls fading, the worries shrinking to indistinct specks; even the constant pressure of guilt was lifted, leaving nothing but Liz and I. Electricity flowed through my veins, twitching and tugging my muscles and sending me into a state of hyper-awareness. The kiss ended all too soon, the glorious embrace over, and I stumbled backwards in a drunken daze. The room came back into view for only a brief second before disappearing again as I cartwheeled backwards onto the ground. I heard Liz laugh, a beautiful, tear-choked melody, music I had never known I yearned for until that every moment. I tried to get back up, but my brain wasyour final warning. Cut your engines, lay down your arms and submit to Enforcer personnel. If you do not comply, we will be forced to employ severe measures. In this event, we will no longer be able to prevent collateral damage. You have been warned.

The message filled me with confidence, both because of my success in re-establishing communications and because it meant that I was no longer alone in this fight. I fought the urge to fly the shuttle down through the gap, to take a peek at what kind of fleet the Enforcement Bureau had mustered. But I couldn't abandon my quest, not yet, not until I had made sure that Ascension were going to pay for their crimes. Instead, I flicked back out of the maintenance


applications on Edward's Comp, searching through the categories and settling on the one marked 'Surveillance'. Inside were even more categories, names of the various sectors in Ark City, but I ignored them completely. What I was interested in lay not within the floating monstrosity, but outside it.

I tapped the icon for 'External' surveillance and was treated to a list of camera locations - recorded as angular measurements in degrees and minutes - that covered the entire hull. I flipped through a few of the streams until I caught a blur of white and blue, and repositioned the camera to get a better look. It was a squat, figure-eight shaped remote-controlled drone, about the size and shape of two large pizzas sitting next to each other. It had numerousthin arms projecting from its side, some sporting cylindrical tubes and others with blue glowing spheres, but all were pointing at something below the view of the camera. I cycled through other streams and found more drones, most of them clustered around the front and rear of Ark City. By manipulating one of the cameras, I was able to get a glimpse of what it was the drones were so interested in.

They appeared to be targeting the giant spherical engines, their weapons trained on the balls as they swivelled in their sockets. There were dozens of these engines, each providing Ark City with the necessary propulsion to keep it afloat, and every single one seemed to have a contingent of drones watching on. Their arms, which I had


Comp, given it was still clipped into the suits wrist jack. Shit! Now I had nothing to protect me from whatever weapons Edward was packing. I sighed, cursing the day's shitty fortunes, and struggled back to my feet.

With nothing to gain from wallowing in sorrow, I turned to the stairs and began to descend. The lights began activating as I drew close, a sliding window of illumination that followed my progress, shadows snapping back into existence behind me as I made my way down. The stairs continued for two flights with no doors, no windows, nothing at all to break up the monotony. Upon reaching the bottom I found myself on another short landing, facing a smaller cousin of the black door above. Only one panel again, fortunately,snapped up and I leapt from my seat. I glared ferociously at Edward, staring through his smarmy expression and into the bottomless blue pits within his eyes.

"WHAT DID YOU SAY? HOW THE FUCK DO YOU KNOW ABOUT MY DAD?" I was shocked at just how angry I felt at Edward's abuse of my father. We'd never been close, and I can barely even remember the last time I called him 'dad' to his face, but some sort of familial instinct in the core of my being kicked in, demanding that I defend the man who brought me into this world and raised me by himself. Edward exhibited no reaction to my shouting, maintaining his half-smile and holding my gaze until my fury deflated and the guilt rose back


goddamn slow on the reaction there," I hissed, watching as the individual engine readouts started to change by themselves. Unfortunately, due to the system being a piece of absolute crap, it assumed that all the engines were online and acted accordingly. This meant that although we were regaining our lateral equilibrium, we were developing a distinct backward pitch, with more engines still active on the fore of the city than the aft.

"Gah! You stupid heap of shit!" I slammed a fist onto the cool surface of the command console, ignoring the pain that shot through my already injured hand and focusing on balancing out the automatic adjustments. There seemed to be a considerable delay before the auto-system could act on any manual adjustments, and I was able tofew faint words from the broadcast before we descended into an underground tunnel and passed out of earshot.

-s far as we can tell, the Enforcers are preparing to disable Ark City's engines. They appear to be employing the recently touted EP line of combat drones, designed specifically in response to the recent bouts of machine degradation that have led to rebe-

We turned left at an intersection in the tunnel, squeezed through a cramped passageway, and ascended a broad flight of stairs onto a stubby landing platform. A solitary shuttle awaited us, not an Enforcer vehicle as I had expected but a recreational one, the kind that only those with more money than sense would purchase.

"We've had to co-opt a few private vessels to cover the ground forces. Most


voice was playing over the footage, his tone urgent and noticeably uncertain.

-have revealed that it did not go according to plan, and are advising all residents in the greater north-west region to evacuate in a calm and orderly fashion. Passenger shuttles are en route, and the Enforcement Bureau has marked the following transport terminals as pickup locations...

The reporter began listing various landing platforms I'd never heard of, but it didn't matter anyway as I had tuned him out. I hadn't noticed it at first, but after the mention of evacuation I realised that Ark City had developed a list, the normally precisely balanced monstrosity now starting to slant almost imperceptibly towards the city below. I was breathless in shock, my mouth agape as I stared transfixed at thein the door, like the ones at Kitaki, remember?"

Derek gently pulled me away from the purse and slid his hand back into mine. "Ah, don't worry about it. They've probably blocked network access here anyway. How about you guys tell me what you've been up to instead?"

With occasional additions from Mum, I told Derek about everything that had happened since we parted ways at the stalk platform in Ark City. At the mention of John and his reluctance to offer assistance, Derek's face twisted into an angry scowl, and he punched his fist into the pillow he was sitting on.

"That prick! I saved thousands of goddamn lives and he was too much of a coward to send help? Man, I should have known. I mean c'mon, he made


forced a confident smile.

"Don't worry, we'll be fine! Just think, this isn't the craziest thing we've done in the last day, is it?" She laughed, though I could clearly hear the straining notes. I gave her quick hug, clutching her tight and relishing the feel of her body pressed against mine, then strode to where Jimmy had stood only moments ago. I looked down at my hands and pulled the silver and black gloves on a little tighter.

"Well, here goes nothing!" I gave Gregor, Liz, and her mother one final glance, gritted my teeth, and grabbed hold of the blue pole with both hands. The black strip on the gloves immediately stuck to the cool surface, adhering with a reassuring strength. I took a deep breath, bent mythe ship itself. The grip of the gloves began to loosen just as I entered the hatch, giving way completely a few seconds later and leaving me to plunge the last metre into the soft embrace of a gigantic white sponge. The pillowy surface sucked me in, and I was overwhelmed by the sudden fear of being completely consumed by this fluffy monstrosity until a cold metal arm grabbed me by the wrist, effortlessly lifting me out and onto my feet.

"Thanks Jimmy! Oww!" I winced as he let go of my arm and I was forced to stand under my own power. My bruised muscles weren't particularly happy with my recent violent exercise, and I gently massaged the side of my hip while I waited for the pain


while the other remained steady. "Unfortunate side effect is little bit of motion sickness. Should pass soon." True to his word, I was already beginning to feel a little better, my stomach settling back into its regular position. Gregor rubbed his hands together and continued.

"So, let's talk Jimmy first. The staff and I hide out from Enforcers, wait for them to leave after destroying store. When I walk out I find Jimmy, body crushed under huge pile of second-story crap. It not right, make me very upset. Jimmy good kid, not deserve this. He still breathing, though, so I think Gregor, you must do something, save innocent little boy." Jimmy sneered and flipped his hand, dismissing Gregor's claim of 'innocence'. "Lucky for me, stupid Ascension soldiers not destroyI noticed that Jimmy hadn't moved from his spot on the couch. He must have sensed my gaze because he turned his head and smiled at me. I pointed to the door and gestured with my thumb but he just smiled sadly and shook his head. I was tempted to question his reluctance, but thought better of it when I remembered he couldn't respond. _That_ was going to take some getting used to.

The three of us left the room and followed in Gregor's footsteps to what turned out to be the cockpit. Gregor was standing next to a black chair, his arm leaning casually on the headrest, our pilot hidden from view. I could see the tunnel whizzing by out of the front view-screen, but the constant rotation


in there." "Nice to be indoors, isn't it?" "You gotta know when to golem" "Finally, I'd like to announce... that starting Monday, this show will be broadcast in HDTV." "Here's how I'll look." "Heh?" "That's right..." "Look at your hero!" "Good night, everybody." "Aren't you coming, Bart?" "Nah, I'm gonna go backstage and complain." "This Krusty Brand alarm clock sprays acid in your face." "Oh!" "You already showed me before!" "Whoa, Krusty's prop room." "Cool!" "It's that clown car all those midgets drowned in." "Wha..?" "Hey, kid.This ain't a museum." "That's a museum." "For kids, $49 bucks." "Krusty, what's that monster?" "It's the Golem of Prague." "Legendary defender of the Jewish people, like Alan Dershowitz, but with a conscience." "I kid Alan." "We're friends." "Anyway, back in themay be bifurcated, but I still have feelings." "I feel so guilty." "I've mangled and maimed 37 people and I told a telemarketer I was busy when I wasn't." "I'm not a good man." "He sure is neurotic for a monster." "The cure to any man's problems is a good relationship." "Yeah, get him a woman." "That'll end his misery." "Ow, ow!" "This hurts so much." "Just write a scroll asking him to stop." "Writing's for nerds." "You got a surprise for me?" "The surprise would be if I found some good live theater in this town." "Then people could have some drinks, watch Fiddler." "Very nice." "Will you shut up." "Is she for me?" "Hanukkah came early this year, which it sometimes does." "Hello, everybody." "Ugh!" "What's


Army." "What's the threat?" " Uh, we've been invaded... by a pompous, radio ham." "How's it feel when the hoax is on the other foot?" "I must admit it's unpleasant." "You fool." "You've doomed us all." "Eh, tell it to the Great Gildersleeve." "Well, they didn't eat the skin." "That's... that's healthy." "Colonel Kang, your report." "Uh, well..." "The Earthlings continue to resent our presence." "You said we'd be greeted as liberators." "Don't worry." "We still have the people's hearts and minds." "I don't know." "I'm starting to think "Operation:" "Enduring Occupation"" " was a bad idea." " We had to invade." "They were working on weapons of mass disintegration." "Sure they were." "♪ I don't want to set ♪ ♪ the world on fire ♪" "♪ Ithe original name of Judge Judy was, in fact, ‘Hot Bench.’ ”

With all that, it is the more than $47 million being paid to the former Manhattan family court judge that makes up the heart of this action. “This figure is grossly inconsistent with customary practice in the television industry, as the next-highest salaries in non-scripted television at that time were David Letterman ($28 million), Jay Leno ($25 million), and Conan O’Brien ($10 million),” the paperwork from Rebel lawyers Freedman + Taitelman, LLP says of the $45 million Judge Judy was being paid as of early 2010.

“And in 2013, when Scheindlin was reportedly receiving $47 million annually, no one else came close, as the next highest salaries in non-scripted television were Jon Stewart ($30 million), Matt Lauer ($25


On the upside, logical convergence coincides with convergence in the n-bisimulation topology – i.e. Proposition 1 fails – when L has finite atoms. This is a corollary to Proposition 5. An alternative to a metric-based approach to topologies is to construct the set of all open sets directly. Comparing the definition of logical convergence with that of convergence in topological spaces is highly suggestive: Replacing every occurrence of the formula φ with an open set U while replacing satisfaction  with inclusion ∈ transforms the former definition into the latter. Hence the collection of sets Uφ = {x ∈X : x  φ}, φ ∈ LΛ, seems a reasonable candidate for a topology. Alas, this collection is not closed under arbitrary unions, as all formulas are finite. Henceby (fk(x0))k∈N0 . Let (c′n)n∈N0 be the subsequence of (cn)n∈N0 where the machine has finished the successor operation and returned its read write head to its starting position ., ready to embark on the next successor step. The tape of the first 9 of these c′n are depicted in Fig. 1. Let (x′n)n∈N0 be the corresponding subsequence of (fk(x0))k∈N0 . We show that (x′n)n∈N0 has uncountably many limit points: For each subset Z of N, let cZ be a tape with content 1 on cell i iff i ∈ Z and 0 else. On the Kripke model side, let the corresponding xZ ∈ X be a model structurally identical to those of (x′n)n∈N0 , but satisfying φ1 on all "cell states" distance i ∈ Z from the designated


distortion of tissue architecture, increased myocardial stiffness, and the heart's inability to rapidly refill with blood in preparation of the next contraction ([@bib10]). In HFpEF, the role of macrophages, which promote fibrosis in many disease settings, is incompletely understood. Specifically, insights into whether changes in cardiac macrophage number or phenotype increase myocardial stiffness may help overcome therapeutic roadblocks.

Here we show that cardiac macrophages expand and have a causal role in diastolic dysfunction. Higher myocardial macrophage densities arise from monocyte recruitment and activation of hematopoiesis in bone marrow and spleen. In mice with macrophage-specific deletion of IL-10, an interleukin that increases during diastolic dysfunction, rapid cardiac filling during diastole improves. IL-10 contributes to a macrophage phenotype shift toward a profibrotic subset, which activates fibroblasts. Fibroblasts become more numerous andfrom control, SAUNA-exposed, and aged mice. Left: Representative flow cytometry plots; right: number of leukocytes and myeloid cells per milliliter of blood. Data are pooled from 2 (aging) to 11 (SAUNA) independent experiments (*n* = 8--89 mice per group). **(B)** Relative expression levels of different chemokines and adhesion molecules by qPCR in hearts from control, SAUNA-exposed, and aged mice. Data are pooled from two (aging) to four (SAUNA) independent experiments (*n* = 10--33 mice per group). **(C)** Flow cytometric quantification of myeloid cell populations in hearts from control and C57BL/6 and *Ccr2^−/−^* SAUNA-exposed mice. Left: Representative flow cytometry plots; right: number of cell populations per milligram of heart tissue. Data are pooled from three independent experiments (*n* = 14--15 mice per group). **(D)** Immunohistochemical analysis of macrophages in


of neutrophils and macrophages in hearts from littermate and *Cx~3~cr1 Il10^−/−^* SAUNA-exposed mice. Left: Representative flow cytometry plots; right: number of neutrophils and macrophages per milligram of heart tissue. The pie charts indicate the percentage of MHCII^low^ (orange) and MHCII^high^ (purple) cardiac macrophages. Data are pooled from two independent experiments (*n* = 14--16 mice per group). **(C)** Immunohistochemical analysis of macrophages in hearts from littermate and *Cx~3~cr1 Il10^−/−^* SAUNA-exposed mice. Left: Representative images; right: bar graph shows percentage of positive staining per ROI. Data are pooled from two independent experiments (*n* = 6--7 mice per group). Bar, 25 µm. **(D)** Hemodynamic parameters by pressure--volume catherization of hearts from littermate and *Cx~3~cr1 Il10^−/−^* SAUNA-exposed mice. Data are pooled from two independent experiments (*n* = 7--11 mice per group). **(E)**receptors of IL-10 superfamily members (IL-10Rβ; [@bib24]), in fibroblasts. qPCR on FACS-purified noncardiomyocytes revealed that only cardiac macrophages express both receptor subunits that are necessary for IL-10 binding ([@bib24]; Fig. S3 B), suggesting that IL-10 indirectly modulates fibroblasts by autocrine activation of macrophages toward a fibrogenic phenotype. FACS-purified cardiac macrophages exposed to recombinant murine IL-10 (rIL-10) expressed more osteopontin (*Opn*; [Fig. 9 C](#fig9){ref-type="fig"}), a matricellular protein and multifunctional cytokine associated with cardiac fibrosis causing myofibroblast differentiation and activation ([@bib19]; [@bib39]). Interestingly, macrophages are also the main source of *Opn* in LV myocardial tissue (Fig. S3 B). We then exposed FACS-purified cardiac fibroblasts to rIL-10 directly or to cell culture medium collected from cardiac macrophages incubated with rIL-10. Cardiac fibroblasts increased expression of type I collagen (*Col1a2*), fibronectin (*Fn1*),


tamoxifen with a separation of 24 h between doses, followed by 1 dose of 2 mg of tamoxifen per week for 3 wk. To quantify protease and MMP activity in hearts, 2 nmol of the pan-cathepsin protease sensor ProSense-680 (PerkinElmer) or 2 nmol of the MMP sensor MMPSense-680 (PerkinElmer) was injected intravenously 24 h before analysis. For BrdU label-retaining pulse-chase experiments, BrdU was added to the drinking water (1 mg/ml) for 14 d before inducing chronic HFpEF with the SAUNA protocol.

Blood pressure, surface ECG, and hemodynamic measurements {#s13}
---------------------------------------------------------

Blood pressure in conscious mice was measured with a noninvasive tail-cuff system (Kent Scientific Corporation). Surface ECG was recorded in anaesthetized mice (1.5% isoflurane in 95% O~2~) by using subcutaneous electrodes connected to the Animal Bio amplifier and PowerLab station (ADor (B220/CD49b/CD90.2/NK1.1/Ter-119)^low^ (CD11b/CD45)^high^ Ly6G^high^. Cardiac macrophages were identified as (B220/CD49b/CD90.2/Ly6G/NK1.1/Ter-119)^low^ (CD11b/CD45)^high^ F4/80^high^ Ly6C^low/int^, cardiac fibroblasts as CD31^low^ CD45^low^ MEFSK4^high^, and cardiac endothelial cells as CD31^high^ CD45^low^.

For a HSPC staining, cell suspensions were labeled with biotin-conjugated anti--mouse antibodies directed against B220 (clone RA3-6B2, 1:100), CD4 (clone GK1.5, 1:100), CD8a (clone 53--6.7, 1:100), CD11b (clone M1/70, 1:100), CD11c (clone N418, 1:100), Gr-1 (clone RB6-8C5, 1:100), IL7Rα (clone A7R34, 1:100), NK1.1 (clone PK136, 1:100), and Ter-119 (clone TER-119, 1:100). This was followed by a second staining for CD16/32 (clone 93, 1:100), CD34 (clone RAM34, 1:33), CD48 (clone HM48-1, 1:50), CD115 (clone AFS98, 1:100), CD150 (clone TC15-12F12.2, 1:50), c-Kit (clone 2B8, 1:100), Sca-1 (clone D7, 1:50), and Pacific Orange-conjugated streptavidin (1:100). LSKs were identified as (B220/CD4/CD8a/CD11b/CD11c/Gr-1/IL7Rα/NK1.1/Ter-119)^low^ c-Kit^high^ Sca-1^high^ and HSCs as (B220/CD4/CD8a/CD11b/CD11c/Gr-1/IL7Rα/NK1.1/Ter-119)^low^


cardiac macrophages with and without neutralizing anti--OPN antibody (10 µg/ml; catalog number AF808; R&D Systems) or anti--TGFβ antibody (50 µg/ml; catalog number AB-100-NA; R&D Systems) was added and incubated for 24 to 72 h under normal growth conditions.

Histology {#s20}
---------

To eliminate blood contamination, murine hearts were perfused with 10 ml of ice-cold PBS. The hearts were then excised and embedded in OCT compound. To detect myocardial fibrosis, frozen 6-µm sections were stained with PicroSirius Red (Sigma-Aldrich) according to the manufacturer's instructions. To identify mouse cardiac macrophages, serial frozen 6-µm sections were prepared, blocked with 4% normal rabbit serum, and incubated with a rat anti--mouse Mac3 antibody (clone M3/84; BD Biosciences) overnight at 4°C, followed by a biotinylated rabbit anti--rat IgG antibody (Vector Laboratories) for 30 min at room temperature.Cape Town's drought has shown us the future of travel – even if it means not flushing the toilet

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Cape Town was last year voted Telegraph Travel readers' favourite cityCredit:
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I’m staring at the murky, jaundiced toilet bowl. I reach for the flush but stop and pull my hand back.

“If it’s brown flush it down, if it’s yellow let it mellow.”

Sorry to be crude, but that would be as much as 13 litres of water gone, a fifth of my daily allowance in a Cape Town struggling to manage the precious resource during the city’s worst drought in living memory.

Only I’ve returned from the South African city and am stood not on the southernmost tip of Africa but in my


bath, and, yes, thought twice about flushing the toilet.

Although such diligent water management is not the norm in the UK and nor so in the majority of places to which British holidaymakers travel, it is surely not too far off.

By now, we are familiar and comfortable with keeping towels off the floor in hotels so that they might be used again the next day, saving water on laundry. And in the same way the national conscience on single-use plastic is changing, our approach to sustainable travel is poised to undergo a revamp, with Cape Town guiding the way.

Mapped: the world's most environmentally friendly countries

Last year, the World Travel and Tourism Council launched its Is It Too Much To Ask? campaign, encouraging tourists to sign up to any ofhis death in August from brain cancer.

“One aspect of the [Vietnam] conflict, by the way, that I will never ever countenance is that we drafted the lowest income level of America, and the highest income level found a doctor that would say that they had a bone spur,” McCain said in 2017. “That is wrong. That is wrong. If we are going to ask every American to serve, every American should serve.”

The daughters of a New York podiatrist came forward last year and said their father may have diagnosed Trump with bone spurs to help him avoid military service.

The daughters of Larry Braunstein, the foot doctor, said they didn't know if he ever examined Trump but that he signed off on the diagnosis as a favor to Fred


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out of the skein.

Once I accepted that this would not be a fabulously exciting knit, but rather more contemplative and repetative, I enjoyed working on it. The repeat is easy to memorize — after a while I only had to occassionally give it my full attention, so that many a useless program was watched. Good clean knitting fun.

(Apologies for a boring post. It’s cold and dark here, which has seemed to send my brain into hibernation.)

In an instance of serendipitous timing, this package arrived on Saturday, as most of the east-coast knit-blogging world was amusing themselves at Rhinebeck. This is only part of the collection of fibery goodnes residing in my closet in WA — all purchased in one way or another while I was 1) in Germanyor 2) in need of storage space. I have no explanations as to why I bought all of this yarn. Nevertheless, it is a lovely surprise. I’m in deep passionate love with each and every skein.

Now, if I can just ward off startitis long enough to finish what’s lingering in my knitting basket. Got to free up the needles, after all.

The koigu version of the mystery sock didn’t really work out — the yarn was much too busy for the pattern. So I frogged and started again with some KnitPicks kettle-dyed sock yarn in gold. I’ve never worked with KnitPicks sock yarn before, but so far, I’m liking the yarn. And not only because of the price: it’s soft and seems quite sproingy. If that’s a description.

Anyhoo. The


pattern is shaping up nicely in the new yarn. Because of my tight knitting troubles and an experiment with US1.5/2.5mm needles, I cast on for the 72-stitch version despite my preference for the 64-stitch cable pattern. Le sigh. Unfortunately, I also prefer socks that fit.

RAF anyone? Well, except without the guns and violence and politics. Much like the Baader-Meinhof Gang, the Single Sock Liberation Movement was not an ideological standpoint I thought I’d be subscribing to anytime soon. But that’s all changed. With this latest foray into single sockdom, I think I have to admit my revolutionary proclivities.

One Gentleman’s Plain Winter Sock [ravel it] in Trekking xxl (color 100) with an afterthought heel and star toe of four points. And one Masonic Lodge sock [ravel it] in Trekking1From all our machines put together, from all our roads charted in miles, from all our accumulated tonnage, from all our arrayed aeroplanes, from our regulations, from our conditioning, not the slightest feeling could emerge. That is of another order, and real, and infinitely more exalted. From all your manufactured thoughts, from all your graded concepts, from all your concerted measures, not the slightest frisson of genuine civilization could result.That is of another order, infinitely more exalted and sur-rational.

4True civilizations are poetic shocks: the shock of the stars, of the sun, the plant, the animal, the shock of the round globe, of the rain, of the light, of numbers, the shock of life, the shock of death. Since the sun temple, since the mask, since the Indian, since


critics.

Meryl Streep's nomination for The Bridges of Madison County is her 10th, which puts her in elite company. Only four other actors have 10 or more nominations: 12 for Katharine Hepburn and 10 each for Bette Davis, Laurence Olivier and Jack Nicholson.

With Kathleen Quinlan's nomination this year for Apollo 13, there are now four nominated actors in Oscar history whose last names begin with Q. Can you name the other three?

Here is another amusing little test for you, regarding Oscar nominees' last names. There are three last names that claim five nominees each over the years, more than any other names.

One of those names claims a nominee this year, Ed Harris. Can you name the four other Harrises nominated for acting Oscars? Can you also name the fiveThe in vitro activity of sulbactam combined with third generation cephalosporins against third generation cephalosporin-resistant bacteria.
The in vitro activity of the beta-lactamase inhibitor sulbactam combined with cefuroxime, cefotaxime or ceftazidime in the ratio of 1:1 was studied against ceftazidime- or cefuroxime-resistant Gram-negative rods and Staphylococcus aureus. Sulbactam enhanced the antibacterial activities of cefuroxime, cefotaxime and ceftazidime against Gram-negative rods. The MIC(90) of ceftazidime against Escherichia coli, Enterobacter cloacae, Citrobacter freundii, Acinetobacter spp. and Pseudomonas aeruginosa was reduced 4-fold and that of cefotaxime against E. coli, E. cloacae, C. freundii and Acinetobacter spp. reduced by 2-4-fold. However, sulbactam did not enhance the activities of cefuroxime, cefotaxime or ceftazidime against S. aureus, enterococci and Stenotrophomonas maltophilia. With the combination of sulbactam and ceftazidime at the ratio of 1:1, 38.4% of