updated value. To use in MySQL database: Uncomment method useInMemoryDatabase() and comment
	 * out call to initializeInMemoryDatabase() and executeDataSet() within onSetupTransaction() .
	 *
	 * @see ProgramWorkflowService#savePatientProgram(PatientProgram)
	 */
	@Test
	public void savePatientProgram_shouldUpdatePatientProgram() {

		Date today = new Date();

		PatientProgram patientProgram = pws.getPatientProgram(1);
		Date dateCompleted = patientProgram.getDateCompleted();
		Date dateChanged = patientProgram.getDateChanged();
		User changedBy = patientProgram.getChangedBy();
		if (null != dateCompleted) {
			//System.out.println("Date Completed: " + dateCompleted);
		}
		if (null != dateChanged) {
			//System.out.println("Date Changed: " + dateChanged);
		}
		if (null != changedBy) {
			//System.out.println("Changed By: " + changedBy.toString());
		}

		patientProgram.setDateCompleted(today);
		patientProgram.setChangedBy(Context.getAuthenticatedUser());
		patientProgram.setDateChanged(today);
		pws.savePatientProgram(patientProgram);

		// Uncomment to commit to database
		// setComplete( );

		PatientProgram ptProg = pws.getPatientProgram(1);
		Date dateCompleted2 = patientProgram.getDateCompleted();
		Date dateChanged2 = patientProgram.getDateChanged();
		User changedBy2 = patientProgram.getChangedBy();

		if (null != dateCompleted2) {
			//System.out.println("Date Completed: " + dateCompleted2);
		}
		if (null != dateChanged2) {
			//System.out.println("Date Changed: " + dateChanged2);
		}
		if (null != changedBy2) {
			//System.out.println("Changed By: " + changedBy2.toString());
		}

		assertNotNull(ptProg.getDateCompleted());
		assertEquals(today, ptProg.getDateCompleted());

	}

	/**
	 * Tests if the savePatientProgram(PatientProgram) sets the EndDate of recent state of each"6c72b064-506d-11de-80cb-001e378eb67e";
		ConceptStateConversion conceptStateConversion = Context.getProgramWorkflowService().getConceptStateConversionByUuid(
		    uuid);
		assertEquals(1, (int) conceptStateConversion.getConceptStateConversionId());
	}

	/**
	 * @see ProgramWorkflowService#getConceptStateConversionByUuid(String)
	 */
	@Test
	public void getConceptStateConversionByUuid_shouldReturnNullIfNoObjectFoundWithGivenUuid() {
		assertNull(Context.getProgramWorkflowService().getConceptStateConversionByUuid("some invalid uuid"));
	}

	/**
	 * @see ProgramWorkflowService#getPatientProgramByUuid(String)
	 */
	@Test
	public void getPatientProgramByUuid_shouldFindObjectGivenValidUuid() {
		String uuid = "2edf272c-bf05-4208-9f93-2fa213ed0415";
		PatientProgram patientProgram = Context.getProgramWorkflowService().getPatientProgramByUuid(uuid);
		assertEquals(2, (int) patientProgram.getPatientProgramId());
	}

	/**
	 * @see ProgramWorkflowService#getPatientProgramByUuid(String)
	 */
	@Test
	public void getPatientProgramByUuid_shouldReturnNullIfNoObjectFoundWithGivenUuid() {
		assertNull(Context.getProgramWorkflowService().getPatientProgramByUuid("some invalid uuid"));
	}

	/**
	 * @see ProgramWorkflowService#getPatientStateByUuid(String)
	 */
	@Test
	public void getPatientStateByUuid_shouldFindObjectGivenValidUuid() {
		String uuid = "ea89deaa-23cc-4840-92fe-63d199c37e4c";
		PatientState patientState = Context.getProgramWorkflowService().getPatientStateByUuid(uuid);
		assertEquals(1, (int) patientState.getPatientStateId());
	}

	/**
	 * @see ProgramWorkflowService#getPatientStateByUuid(String)
	 */
	@Test
	public void getPatientStateByUuid_shouldReturnNullIfNoObjectFoundWithGivenUuid() {
		assertNull(Context.getProgramWorkflowService().getPatientStateByUuid("some invalid uuid"));
	}

	/**
	 * @see ProgramWorkflowService#getProgramByUuid(String)
	 */
	@Test
	public void getProgramByUuid_shouldFindObjectGivenValidUuid() {
		String uuid = "eae98b4c-e195-403b-b34a-82d94103b2c0";
		Program program = Context.getProgramWorkflowService().getProgramByUuid(uuid);
		assertEquals(1, (int) program.getProgramId());
	}

	/**
	 * @see ProgramWorkflowService#getProgramByUuid(String)
	 */
	@Test
	public void getProgramByUuid_shouldReturnNullIfNoObjectFoundWithGivenUuid() {
		assertNull(Context.getProgramWorkflowService().getProgramByUuid("some invalid uuid"));
	}

	@Test
	public void getState_shouldGetStateAssociatedWithGivenIdIfWorkflowStateIdExists() {

		final Integer EXISTING_WORKFLOW_STATE_ID = 1;

		ProgramWorkflowState state = pws.getState(EXISTING_WORKFLOW_STATE_ID);

		assertNotNull(state, "ProgramWorkflowState not found");
		assertThat(state.getId(), is(EXISTING_WORKFLOW_STATE_ID));
	}

	@Test
	public void getState_shouldReturnNullIfGivenWorkflowStateIdDoesNotExists() {

		assertNull(pws.getState(99999));
	}

	/**
	 * @see ProgramWorkflowService#getStateByUuid(String)
	 */
	@Test
	public void getStateByUuid_shouldFindObjectGivenValidUuid() {
		String uuid = "92584cdc-6a20-4c84-a659-e035e45d36b0";
		ProgramWorkflowState state = Context.getProgramWorkflowService().getStateByUuid(uuid);
		assertEquals(1, (int) state.getProgramWorkflowStateId());
	}

	/**
	 * @see ProgramWorkflowService#getStateByUuid(String)
	 */
	@Test
	public void


for a harsh world.

He also rides a horse well and dresses in bright colors.

This is rightly considered a classic (Number 7 on the Big List of 1000 Movies). The photography is spectacular, the action exciting, the story morally ambiguous, and the acting is mostly great.

“Solo Filmschool” movies are those on the big list of the 1000 best films of all time, which the crew over at TSPDT keeps track of and updates from time to time. The current version is from January 2010. My plan is to work my way down the list, watching all of them on DVD (if available), regardless of how slow-moving, or out of date they might appear at first. If a highly-regarded and serious film class is not available where you live, youSNORD113 (9 copies) and SNORD114 (31 copies) families \[[@R89], [@R90]\] (Figure [1](#F1){ref-type="fig"}). The SNORD112 and some of the SNORD113 members are located in the intron of MEG8 gene. Like what has been observed in MEG3, some snoRNA expression levels were also decreased in AML and ALL cells compared to normal cells \[[@R91]\]. Opposite to the effect of MEG3, over-expressing SNORD114 member induces K562 and HCT116 cell proliferation \[[@R92]\] and suppressing the snoRNA induces cell death. Further studies revealed that SNORD114 promotes cell cycle progression through G0/G1 to S phase transition \[[@R91]\]. The level of SNORD114-3, one of the snoRNAs in the DLK1-MEG3 snoRNAs cluster showed good correlation with MEG3 expression, which suggests the SNORD114-3 and MEG3 probably are co-regulated by the IG-DMR and MEG3-DMR regions. These findings indicate


the prognostic outcome of different cancers. For example, Shih et al. reported 29 miRNAs that were associated with disease outcome in advanced ovarian cancer patients and 11 of the 29 miRNAs are located in the DLK1-MEG3 cluster \[[@R99]\]. Nine of those 11 miRNAs including miR-433, miR-127, miR-381, miR-377, miR-299-3p, miR-409-3p, miR-154, miR-382, and miR-376c are associated with OS. In a separate study, the increase of miR-376c level in ovarian cancer cells was found to inhibit cisplatin induced cell death \[[@R100]\]. The miRNAs in this region have also been shown to be involved in the progression of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC) \[[@R101]--[@R105]\]. In addition, miR-495, miR-134, miR-409-3p, miR-496, miR-379, miR-369-3p in the cluster are linked to the tumor invasion depth in gastric cancer \[[@R104],can change the ratio between MDM2 and TP53 \[[@R117]\]. These findings indicated the down-regulation of MDM2 is one of the mechanisms for MEG3 to affect TP53 dependent transcription. Therefore, MEG3 induced apoptosis and anti-proliferative activities in cells may be mediated through the suppression of MDM2 and subsequent activation of TP53 signaling pathway \[[@R115], [@R122]\].

### MEG3 and GDF15 {#s1_4_3}

MEG3 also enhances a TP53 dependent expression of growth differentiation factor 15 (GDF15), an inhibitor of cell proliferation, which is a member of the transforming growth factor-β (TGFB) superfamily (Figure [1](#F1){ref-type="fig"}) \[[@R123]\]. Studies reported that GDF15 inhibits proliferation of several cancer cell lines *in vitro* as well as suppresses tumor formation *in vivo* \[[@R124], [@R125]\]. Zhou et al. demonstrated that GDF15 can be directly affected by MEG3 since re-expression of MEG3


genes involved in VEGF angiogenic pathway in a MEG3 KO mice brain compared with normal wild type \[[@R81]\]. The increase of VEGF pathway activity in MEG3 KO mice brain suggests that MEG3 may play an important role in the progression for tumors like meningioma. In addition, one recent study showed that overexpression of MEG3 suppresses cell proliferation and promotes apoptosis by reducing the Wnt/β-catenin pathway activity in retinoblastoma cell lines. Treating the cells with a Wnt/β-catenin pathway activator reverses MEG3 over-expression induced anti-proliferation activity in retinoblastoma cell lines \[[@R77]\]. Using a modified chromatin oligo affinity precipitation method, Mondal et al. found some of the genes involved in TGF-β pathway interact directly with MEG3 \[[@R134]\]. It has been suggested that MEG3 regulates the expression of its target genes throughbeen widespread international outrage over the bloodshed, in which dozens of children also lost their lives -- many of them, according to eyewitnesses, having been shot in the head at close range. Nevertheless, it appears unlikely that the UN will authorize a military intervention. Both China and Russia on Wednesday reiterated their opposition to such a move.

Their comments came as French President François Hollande indicated on Wednesday that he would not rule out the possibility of a military intervention in the country. "It is dependent on me and the others to convince the Russians and the Chinese" not to veto military action in the United Nations Security Council, Hollande said. "We cannot allow Syrian President Bashar Assad to continue massacring his own people." Hollande plans to meet with


be willing to accept samples. Here’s a template you can use:

Hi Janet,

My name is Lauren, and I’ve been a big fan of your Instagram posts.

You might be interested to know that I’ve recently launched an online store around (the type of product you sell). I’m particularly proud of (the product that you’re sending), and wonder if you might accept if I pass along a sample of it. If you like it as much as I do, perhaps you’ll consider sharing it with your audience?

Please let me know if there’s a business address for me to send a package to, and if you have any questions at all.

Lauren

yoursite.com

2. Reach out to bloggers and press

There are other people you can reach out to: bloggers, vloggers, and press. The size ofthe word about it? I’d really like to get some momentum going.

Craig

Add exclamation marks as required.

5. Pro-actively engage on Twitter

You don’t need to start selling products before you engage with people on Twitter. There are a lot of ways to get on peoples’ radars as a cool new store.

That’s nothing to sneeze at. How did it get a few hundred followers pre-launch?

First, it generated some follow-backs by following select people on Twitter whom it determined may be interested in a new bakery. These include restaurateurs and self-declared bread enthusiasts in Toronto.

One tool that you can use to find relevant users is Followerwonk. Log in through Twitter, and you can search relevant keywords in bios and profiles.

It has also been following a disciplined tweeting strategy. Blackbird has been baking


Meretz leader Zahava Gal-On took aim at the premier's intention to pass a law allowing Israelis abroad to vote in parliamentary elections.

Prime Minister Benjamin Netanyahu’s intention to enact a law allowing Israeli nationals living abroad to vote in parliamentary elections is a ploy to dilute the political influence of the country’s Arab citizens, the head of the left-wing socialist Meretz party said on Friday.

Zahava Gal-On told Israel Radio on Friday that she finds it “hard to reconcile with the fact that close to 1 million Israelis who chose to live outside the country’s borders and who do not serve in the IDF reserves and do not pay taxes can exert their influence on the government’s policies without having to bear the consequences of those policies.”

The Meretz chair saidas happy to watch Doug McClure fight stiff puppet dinosaurs in Edgar Rice Burroughs' adventures produced by Amicus Films. This was the sequel to The Land That Time Forgot.

September, Film Review

After the success of The Golden Voyage of Sinbad, Harryhausen made this third Arabian Nights adventure, Sinbad and the Eye of the Tiger. Coincidentally, John Wayne's son Patrick starred in both this and People That Time Forgot.

August, Films and Filming

You see? This looks quite exciting, and the cast was superb for just a sequel (Richard Burton, Louise Fletcher, Max Von Sydow, James Earl Jones). But, John Boorman's Exorcist II: The Heretic was one of the early culprits that gave sequels a bad name.

October, Film Review

I think i saw Exorcist II, then The Exorcist on its five year anniversary


re-release. Not the recommended way of seeing them at all.

Annnnd, the Jaws rip-offs continued blithely on along with many other 'animal attack' movies, until they were swallowed up by outer space. Orca - The Killer Whale was the most expensive movie in the wake of Jaws, Dino De Laurentiis mixing killer killer whales with elements of Moby Dick and Frankenstein. Besides Richard Harris and Charlotte Rampling, Bo Derek makes an early appearance.

September, Photoplay Film Monthly

More Jaws. Roger Moore's third James Bond The Spy Who Loved Me was the biggest summer hit that year. But Roger Moore's Bond films always seemed to be copying other movie trends rather than setting them. While the producers harped on about the huge new soundstage with three submarines parked in it, it wasn'tTrends of maxillofacial injuries resulting from physical violence in Brazil.
A large proportion of interpersonal violence results in maxillofacial injuries. The monitoring of maxillofacial injuries in the context of gender violence has been little explored. The aim of this study was to analyze trends in cases of maxillofacial injuries resulting from interpersonal physical violence considering the gender of the victim and perpetrator. A time-series study was conducted. Data regarding maxillofacial injury cases due to interpersonal physical violence were collected from reports at a forensic institute of a city in northeastern Brazil, between 2008 and 2014 (84 months). Rates of interpersonal physical violence per 100 000 inhabitants were determined for each gender. A negative binomial regression model was used to evaluate trends. The cycle plot was used to investigate the


Q:

How to "take a photo" of a frame that was created in the code behind during run-time, in WPF?

I have this function, which is called from a button Click event handler:
private void CreateFrame(Page page)
{
    Frame newFrame = new Frame();
    newFrame.Navigate(page);

    // FramesHolder is a StackPanel with multiple Frames inside it
    FramesHolder.Children.Add(newFrame);

    // The size the page is 525x50
    RenderTargetBitmap renderTargetBitmap =
        new RenderTargetBitmap(525, 50, 96, 96, PixelFormats.Pbgra32);
    renderTargetBitmap.Render(newFrame);
    PngBitmapEncoder pngImage = new PngBitmapEncoder();

    pngImage.Frames.Add(BitmapFrame.Create(renderTargetBitmap));
    using (Stream fileStream = File.Create("Frame.png"))
    {
            Frame newFrame = new Frame();
        newFrame.Navigate(page);

        // The size the page is 525x50
        RenderTargetBitmap renderTargetBitmap =
            new RenderTargetBitmap(525, 50, 96, 96, PixelFormats.Pbgra32);
        renderTargetBitmap.Render(page);
        PngBitmapEncoder pngImage = new PngBitmapEncoder();

        pngImage.Frames.Add(BitmapFrame.Create(renderTargetBitmap));
        using (Stream fileStream = File.Create("c:\\Frame.png"))
        {
            pngImage.Save(fileStream);

        }


  line-height: 25px;
  }

  .log-event:hover {
    border-style: solid;
    background: #eee;
  }

  .log-event.pinned {
    border-style: solid;
    background: #eee;
    opacity: 1;
  }

  .player0 {
    border-left-color: #ff851b;
  }

  .player1 {
    border-left-color: #7fdbff;
  }

  .player2 {
    border-left-color: #0074d9;
  }

  .player3 {
    border-left-color: #39cccc;
  }

  .player4 {
    border-left-color: #3d9970;
  }

  .player5 {
    border-left-color: #2ecc40;
  }

  .player6 {
    border-left-color: #01ff70;
  }

  .player7 {
    border-left-color: #ffdc00;
  }

  .player8 {
    border-left-color:step.

In this section, you need to provide the name and a product. The description and quantity is optional.

After adding the rewards, you will be asked to state how many points should a customer receive in order to be eligible for a reward.

Create three rewards in total for respectively a badge (worth 25 points), water bottle (worth 150 points) and a t-shirt (worth 400 points).

In the 4th step, you may optionally launch a distribution (notification) to inform your customer about respectively three events: to send a loyalty program sign up code, to let them know about points they've got and about rewards they've received. You can set up different distribution channels for each of these messages.

In the 5th step, you may provide metadata - custom fields to help with


are not frozen out even there. As a result, the linear specific heat coefficient $C(T)/T$ and the impurity susceptibility were found to diverge logarithmically. A further surprising result arose from the conformal field theory approach: Affleck and Ludwig [@C] showed that (i) the impurity contribution to the resistivity shows a $\sqrt{\max\{\omega,T\}}$ singularity (as opposed to the $\sim \omega^2, T^2$ Fermi liquid behavior) (ii) that the amplitude of scattering to a one-electron state vanishes at $T=\omega = 0$, and an incoming electron “evaporates” to infinitely many electron-hole excitations once it hits the impurity [@B; @C] (see Table \[table\]).

It must be emphasized that for $n=2$ an arbitrarily small splitting of the impurity states (produced by magnetic field or a strain field for dynamical defects) provides a cutoff for the non-Fermi liquidwith the experiments [@BG; @exp1]. For $S=1/2$ spin alloys (e.g. ${La}_{1-x} {Ce}_x$ films) no size dependence is expected due to the surface anisotropy in agreement with the experiment of Ref. [@Roth].

The proximity effects [@prox; @proxdis] can be well explained by the surface anisotropy as the number of available spin-orbit scatterers is increased by the overlayer and the magnetic impurities are in further distances from the surface of the samples, but only if the overlayer is in the ballistic region as well. In a new experiment of Giordano [@Giordanolayer] different multilayers composed of $Au$ and $Au(Fe)$ films were examined (where the overlayer was positioned only on one side, or on both sides of the film), giving good agreement also quantitatively with the predictions of the theory of surface anisotropy.

There are


found between the calculated and experimentally determined anomalous amplitude of the Kondo signal (see Fig. \[fig:sizedep\]).

It was also predicted by the theory [@ZarandUdvardi] that this effect should be much less pronounced for alloys with large $T_K$ as $T_K$ is less sensitive to the change in $\varrho_0$ in that case (see Eq. (\[eq:T\_K\])), which has indeed been later confirmed by the experiments studying $Cu(Fe)$ alloys [@A].

Kondo resonance in the density of states measured by STM {#S2C}
--------------------------------------------------------

It has been known for a long time [@Mezei] that the local electron density of states nearby a magnetic Kondo impurity has a specific structure due to the Kondo resonance. In the early experimental attempts a change in the electron density of states due to a layer of dilute magnetic alloys fabricated inside a metal hasa way where it is proportional to $q^2\sim {|(\Phi|T|i)|^2}/{|(\Psi_E|T|i)|^2}$. The discrete level and the continuum state of energy $E$ have the wave function $\varphi$ and $\Psi_E$, respectively, and there is hybridization between them with amplitude $V_E$. The initial electron state $|i\rangle$ has a transition described by operator $T$ to the exact states including the hybridization. The discrete state $\Phi$ in definition of $q^2$ is, however, not the original unhybridized state $\varphi$, but the state modified by the hybridization as $\Phi=\varphi+{\mathcal P}\int dE' {V_{E'}\Psi_{E'}} (E-E')^{-1}$ [@Fano]. Thus $(\Phi|T|i)$ can be different from zero even if $(\varphi|T|i)=0$, i.e., thus even without direct transition to the localized state $\varphi$, a transition rate still exists into the state $\Phi$. That clearly shows that Fano line shape can be obtained without direct transition


of about $\sim 0.4\AA$, and was found to be in the range $\sim0.01-1K$. It has been suggested that virtual hopping to the lowest excited states could increase $T_K$ substantially [@ZarZawTLS], however, in the above model this turned out to be wrong [@Altshexc; @Zarconf]. It has been shown that, in reality, $T_K$ is reduced even further if one includes the effect of [*all*]{} the excited states. Thus, within the original simplistic TLS model, where the TLS is formed by some heavy atom tunneling between two close positions and electron-hole symmetry is assumed, it seems to be impossible to have $T_K$ in the experimentally observed range. It has been pointed out recently, that the criticism of Ref. [@Altshexc] is essentially based on the assumption of electron-hole symmetry in the conduction electroninteresting question about the electronic dephasing time $\tau_{\phi}$. This is the time scale for an electron to stay in a given exact one-electron state in the presence of static impurities. The transitions between these states are due to electron-phonon, electron-electron, electron-dynamical defects (e.g. two-level system), or electron - magnetic impurity interactions. At low temperature the electron-phonon interaction freezes out and the electron-electron interaction becomes dominant which has been studied by Altshuler and his collaborators [@altshuler]. According to that theory the dephasing time tends to infinity as the temperature is lowered, as the available phase space for the electron-electron scattering gradually vanishes. However, as recently emphasized by Mohanty and Webb [@mohanty], experimentally this is not always the case. In some materials and samples, like $Ag$ produced by the Saclay


group [@saclay], $\tau_{\phi}$ follows very closely the predictions of the electron-electron interaction theory but in other cases there are strong deviations from the predicted behavior and the data indicate some saturations (see Fig. \[figsat\]).

It has been known since a long time that the saturation-like behavior depends very much on the preparation of the samples [@label4] and even on the substrates on which the films are deposited [@label5]. The early suggestion by Lin and Giordano [@label4] was that the dephasing is either due to the magnetic impurities or some defects which are very sensitive to the metallurgical properties of the films including thickness, annealing etc. The effect of magnetic Kondo impurities on the dephasing rate was carefully studied in those cases where the Kondo temperatures were in the relevant temperaturein degenerate semiconductor ballistic quantum dots [@label11]. In degenerate semiconductor as far as we know even the theory of magnetic Kondo effect has not been worked out in detail.

Energy distribution of electrons in short wires {#S3D}
-----------------------------------------------

In addition to the dephasing problem another closely related dilemma exists concerning the energy distribution of electrons in short wires with finite bias voltage. In these experiments [@label12; @label13] one measures the energy distribution function of electrons $f(E)$ by fabricating a metal-metal oxide-superconductor (M-MO-S) tunnel junction at various positions along the wire (see Fig. \[figmmos\]).

0.4truecm

From the $I-V$ characteristic $f(E)$ is determined by deconvolution. The typical length of the wires was $1.5 \mu m$ and $5 \mu m$. The measurements were carried out at $25 mK$, and the samples were in the diffusive limit. In


his post.

6.When criticized by the media, Pachauri has a track record of responding unprofessionally.

He has spoken of the “scurrilous writings by some journalists,” the “mal-intent of those who are behind the falsehoods” and “ill-founded criticism.” In his view, the IPCC has been “belittled” by “misleading” and “irresponsible” reporting.

.

7. The independent review said the IPCC was too insular and could benefit from “a greater variety of perspectives.” It recommended the establishment of a new, Executive Committee that would include “three independent members,” particularly individuals “from outside of the climate community.”

Pachauri’s IPCC has, indeed, established such a committee, but it includes no outsiders. Instead, IPCC employees fill those three slots.

.

8. Nine weeks prior to the release of the independent review’s findings, the Pachauri-led IPCC announced the names of the experts… Continue reading →

The WEGOHEALTH Activist 30-Day Writing Challenge,Day 28 “5 Challenges & 5 Small Victories. Make a list of the 5 most difficult parts of your health focus. Make another top 5 list for the little, good things (small victories) that keep you going.” … Continue reading →

The WEGOHEALTH Activist 39 Day Writing Challenge, Day 22 “Tip Tuesday! Have you mastered the hashtag? Figured out the Instagram algorithm? Or maybe you have a few tips to increase your blog followers. Whatever it is, share your expertise with … Continue reading →

The WEGOHEALTH Activist 30-Day Writing Challenge, Day 20 “What has been the highlight of your health activist journey? We want to hear all about it, perhaps a conference, a podcast or meeting a fellow HA. What made


curdled yesterday." "And today as well." "That's not a good omen at all." "Mom." "Mom, sit." "Take a seat." " Sit." "Sit, let me explain it to you." " But you know, it's.." "Sit, let me explain it to you." "Look, brother and I used all of our savings to buy this flat." " Yes." "Now, this flat won't be ours.." ".. until we don't repay the loan in the next 20 years." "So, forget all the bad omens." "Because we are going to live in this house happily.." "..for the next 20 years." " Yes." "Brother, please tell her." " Of course." "Uncle." " Young man, that's not a toy." "Your uncle had to pay a lot of money to buy this." "Go to school." "Come on,"." "You are just.." "What do you think?" "." "I am hurt." "Get some ice." " I'll get it right away." "Mummy, see what happened." " What happened?" "." "What happened?" "." "I got hurt." " What was the need?" "." "You came home late from work." "You should be resting.." "And you are doing this." " What a wonderful mother." "Don't be silly." "No, nothing." " Mannu, you.." "Here, keep this." "God." " It must be really painful." "It's the 21st Manohar." "Remember to pay the flat maintenance fee." "That's all." "What's this?" "." "Lower your feet." "Come on, your mummy is waiting with the milk." " Papa, Papa." "And you all are just.." "Papa, Papa." "Brother, the milk has curdled again." "Since we arrived in this


that's covered with clouds.."" ""You will find it only in your dreams."" ""l have asked the stars to sparkle."" ""Call out my name once more."" ""Let your desires fly."" ""Let's share our happiness."" ""Wait."" "Divya." "Where are you going?" "." "Will you wear these clothes for the marriage?" "." "Marriage?" ".!" "Who's going for the marriage?" "." "I am going to college." "Today my results are coming out." "It's no use." "We know what's going to happen." "Why are you wasting petrol and money?" "." "In the last three years you haven't passed a single subject." "And she won't all her life." " Mother, you are just.." "Come here." "Come here." "You know we all love you so much." " Are you taunting me?" "." "Can't you doin the last episode." "What happened?" "." "Why do bad things happen to good guys?" "." "She had such faith in Lord Ganesha." "And the bad ones are always depicted well." "Mummy, it's just a programme." "Why are you so tense?" "." "I kept something in the kitchen." "Keep it in the fridge." "Go." " Fine." "Come back soon." "You are sneaking a peek and feel ashamed to watch the show with us." "Come out, come on out." "No one will say anything." " No, Priya." "listen to me.." "Mannu, are you fine?" "." "What's wrong with you?" "." "Hello." "Hello!" "Hello." "Hello!" "Priya." "Priya." "Priya." "What's wrong?" "." "Priya." "What happened?" "." "Priya." "Priya." "Priya." "Priya." "Listen to me." " Priya.." "Sister-in-law." " Priya." "Quickly call the


in this house?" "." "Right now." " Didn't you hear anything last night?" "." "No suspicious character?" "." " No, sir." "No screaming nor yelling?" "." " No, sir." "It was nothing like that." "It's altogether a different situation." "A ghost is responsible for this massacre." "Ghost." "Really?" "." "You seem to know a lot." "I know more." "The daughter of this house." "Chitra.." "The one that read news on television." "She was very good looking, like a movie star." "He fell for her after seeing her on television." "Who is he?" "." " The one that follows him." "And misbehaves as well." "That day, on the day of engagement.." ".. he came here, barged inside the house." "He created such a racket." "No one could do anything."Monday." "Priya, listen to me." "Listen carefully." " What is it?" "." "Is brother at home?" "." " I think so." "Priya, lock all the doors and windows of the house." " Why?" "." "No matter what happens, whoever it is, no matter what he says.." "..don't open the doors and windows." "But what's wrong?" "." " Listen to me." "When I come home, I will ring the bell thrice." "Open the door only then." "You are trying to scare me, right?" "." " Please don't argue with me." "Just do as I say, Priya." "Please." "Shiva." "Shiva, someone's going to come to my home.." "..and murder everyone." "You go home." "I will go to the police station.." "..and come back with the force." "We will surround the


place from where I cannot escape." "Or else, the other option is that you kill me." "What nonsense?" "." " No, look." "I am being practical." "Listen to me." "I am not being emotional." "And if anything happens to my family because of me, I will die as it is." "And Doctor, before I do anything, just kill me." "Just kill me." "This is nonsense." "Calm down." "No, you calm down." "Shiva, try to explain it to him." "Mannu, relax." " Just a second, calm down." "Calm down." "I will talk to him." " Okay." "Doctor, I have an idea." " What?" "." "Let's send his family far away without telling him." "Then he wouldn't be able to do anything, even if he wants to." "Because he just---   . beat:        OFF
-- ******* ----
--- ***** ----- [Queues]
 --------------   . celery:      exchange:celery (direct) binding:celery

[Tasks]
  . tbAnalytics.models.processAnalysis
  . tbCollections.models.processCollection

[2011-12-05 19:40:13,558: INFO/PoolWorker-1] child process calling self.run()
[2011-12-05 19:40:13,562: WARNING/MainProcess] celery@ip-10-212-66-181 has started.
[2011-12-05 19:40:13,564: ERROR/MainProcess] Consumer: Connection Error: [Errno 111] Connection refused. Trying again in 2 seconds...
[2011-12-05 19:40:15,574: ERROR/MainProcess] Consumer: Connection Error: [Errno 111] Connection refused. Trying again in 4 seconds...

Tracing it back, it looks like the rabbitmq server is the problem, and the database in particular:
$ sudo rabbitmqctl status
Status of node 'rabbit@ip-10-212-66-181' ...
Error: unable to connect to node 'rabbit@ip-10-212-66-181': nodedown
diagnostics:
- nodes and their ports on ip-10-212-66-181: [{rabbitmqctl14448,38289}]
- current node: 'rabbitmqctl14448@ip-10-212-66-181'
- current node home dir: /var/lib/rabbitmq
- current node cookie hash: 5+uQ077En5bpvle3HJCQMg==

But I haven't


of survival? as opposed to what's commonly considered the communist and socialist means of survival where the two hunters would've shared the deer, where the two states would've shared the land, and where the two bakers would've been working together.

At 11/4/2011 5:07:20 PM, badger wrote:capitalism = people competing against each other.socialism = people working together

logical basis?

Socialism- 100 million deadCapitalism- ????

Competition=/=murder

say you've a deer and two opposing cavemen hunters... one hunter kills the other and wins himself the deer.. that's not capitalism? say you've two states... they go to war over land, one state destroys other and wins the land? that's not capitalism? say you've two opposing bakers... one outbakes the other... the other loses his means of survival, starves and dies (say he was allergic to bread orthe task, that's the toil."

At 11/4/2011 5:07:20 PM, badger wrote:capitalism = people competing against each other.socialism = people working together

logical basis?

Socialism- 100 million deadCapitalism- ????

Competition=/=murder

say you've a deer and two opposing cavemen hunters... one hunter kills the other and wins himself the deer.. that's not capitalism? say you've two states... they go to war over land, one state destroys other and wins the land? that's not capitalism? say you've two opposing bakers... one outbakes the other... the other loses his means of survival, starves and dies (say he was allergic to bread or something) ...that's not capitalism? fair enough, the murdering was less direct in the last example, but still there... all scenarios presented still bear striking resemblance to each other, enough so surely to be categorised under


       1
           Judge Russell C. Hinson signed the order; shortly thereafter, the case was transferred to a different division;
at that time the statute required the convicting judge, if available, to hear post-conviction claims of ineffective assistance
of counsel. See Tenn. Co de A nn. § 4 0-30 -103 (repe aled 1 995 ).

                                                            Q:

Get map location of viewport with angular google maps

I am using this plugin. I am trying to get location lat long of viewport. What I am doing is adding event, because I need that info on every change and trying to figure it out how to pull that data of map object.
JS:
$scope.events = {
  bounds_changed: function(pos) {
    console.log('pos');
    console.log(pos);
  }
}

HTML:
<ui-gmap-google-map events="events"></ui-gmap-google-map>

But inside that map object i can't find something that can help me out.

A:

Actually the solution was in documentation.
Javascript:
$scope.bounds = {}:
$scope.events = {
  bounds_changed: function() {
    console.log($scope.bounds);
  }
}

HTML:
<ui-gmap-google-map events="events" bounds="bounds"></ui-gmap-google-map>


Q:

is it ok to dispose the dataset in finally block and then returning the dataset?

So I am taking over an existing project where the previous coder did many funny things.
What I see the most and not really understand is the following block of code
finally
{
   if (conn != null)
   {
       conn.Close();
       ds.Dispose();
   }
}
return ds;

VS2010 isn't complaining, and the project works as intended, however this is bizzare to me.
How can it be disposed and then returned?  unless the finally takes place after the return somehow?!
If someone can please explain why this is legal? or any other explanation would be appreciated.

A:

Its not correct do dispose ds before returing it from  methodown nation of a crime against humanity.

Oh, he didn’t say it was his fault. But isn’t the serving leader of a country responsible for the state-sanctioned, deliberate slaughter of an entire ethnic group?

So if not him, then who’s to blame? The nice old lady who does my dry cleaning?

Or maybe Trudeau is implying his predecessors in Canada’s top job should carry the can. Well, those folk must be overjoyed to know they might be dragged into the same dreadful category as Hitler, Stalin, Pol Pot, Mao and Idi Amin.


many peoples continue to celebrate their own cultural New Years, in addition to observing the near universal New Yearof 1 January. The Chinese celebrates the start of their lunar year each year some time in January-February, the Thais their Songkran on 13 April, and the Ethiopianstheir Enqutatash on 11 or 12 September –to cite just a few examples. The world can only be the richer with the celebration ofthese cultural diversities.

I wish all readers of this article, the practitioners of Sage Vita, and in fact one and all – a Joyful New year. May we findgreater peace and the ability to createprosperity that can be shared by all the peoples across the globe.

About Sage Vita

Sage Vita, which means a life of wisdom, advocates a lifestyle that will enable          let publicSourcePath = "./samples/" + sampleFolder + "/public/**/*";
            let copyToPath = "./build/samples/" + sampleFolder + "/public";
            return gulp.src([publicSourcePath])
                .pipe(gulp.dest(copyToPath));
        });

        return mergeStream(tasks);
    }

    private getFolders(dir: string): string[] {
        return fs.readdirSync(dir)
            .filter(function (file) {


state of the packaged package.json file to public.
     */
    @Task()
    public packagePreparePackageFile() {
        return gulp.src("package.json")
            .pipe(replace("\"private\": true,", "\"private\": false,"))
            .pipe(gulp.dest("./build/package"));
    }

    /**
     * Copies README.md into the package.
     */
    @Task()
    public packageCopyReadme() {
        return gulp.src("./README.md")
            // .pipe(replace(/```typescript([\s\S]*?)```/g, "```javascript$1```"))
            .pipe(gulp.dest("./build/package"));
 */
    @MergedTask()
    public localCompile() {
        const tsProject = ts.createProject("tsconfig.json", { typescript: require("typescript") });
        const tsResult = gulp.src(["./src/**/*.ts", "./node_modules/@types/**/*.ts"])
            .pipe(sourcemaps.init())
            .pipe(tsProject());

        return [
            tsResult.dts.pipe(gulp.dest("./build/local")),
            tsResult.js
                .pipe(sourcemaps.write(".", { sourceRoot: "", includeContent: true }))


Q:

Is CiviCRM a 'master database' / 'master system'?

The membership organisation I work for currently has serious issues with it's membership databases. That information is currently split between a wordpress user database with an extension s2pro which extends that, mailchimp, paypal, and an accounts package KashFlow.
Those all have various relations with each other but somehow seem to conflict and don't work properly - resulting in the system unsubscribing people from mailchimp, and various other problems with data not matching up.
My manager thinks that what we need to sort this out is a new 'master database' for the membership information which would communicate directly with all the other software packages and be an authoritative place for the data, rather than them all talking to each other and conflicting.
I'm wondering, wouldRobert Wilson (music entrepreneur)

Robert Allan David Wilson, MBE, born in 1951 in the Scottish Borders, is an entrepreneur, musician and philanthropist who lives in Knebworth, Hertfordshire.

He was awarded an MBE in the Queen's Birthday Honours, 2016  for services to the music industry and charity.

Music industry

Until May 2018, Wilson was the Chair of Sound Technology Ltd which he founded in February 1978. It is currently one of the largest independent distributors of musical instruments and professional audio products in the UK and Republic Of Ireland and earned a lifetime achievement award by NORD in 2018.

For the last 40 years, Wilson has been Director of the Music Industries Association (MIA) a UK trade association. It is the only one servicing and representing the interests of all UK businesses selling


For achieving optimum performance, a supply voltage for an electronic component (e.g. a semiconductor component) or an electronic circuit (e.g. a semiconductor circuit) may exceed a nominal supply voltage level. A supply voltage higher than the nominal supply voltage may lead to a reduced life-time of the electronic component or circuit, i.e., a reliability of the electronic component or circuit may be reduced. For example, for a transistor, exceeding the nominal supply voltage level may cause a variety of degradation processes and lead to transistor wear. The transistor may, e.g., be subject to hot carrier degradation (HCD) due to hot carrier injection in the transistor's semiconductor material, bias temperature instability (BTI), or time dependent dielectric breakdown (TDDB).
For monitoring the reliability of an electronic circuit, replica circuits which mimicand finally, the piston-fillers. WWII had a devastating effect on the then very successful company, but by 1945 they were back in limited production. In the 1950’s they made wonderful pens but priced too high for the market and they failed. By the 1960’s, Soennecken was producing only economy pens. They merged with the French company Bayard to produce new pens but those too failed and Soennecken closed its doors in 1967.

The 1913 vintage Soennecken Model 11820 Safety eyedropper-fill is a 14K Solid Gold Overlay on black hard rubber. It has an alternating wave-chased and plain paneled finish. The model number on the pen is covered by the overlay. I do believe this is the “20″ size, the largest size manufactured.

Features an extra-fine/fine nib. Minor dents and dings


Forge (disambiguation)

A forge is the hearth where the blacksmith keeps the fire for heating metals to be formed by plastic deformation, usually with hammer on an anvil.

Forge may also refer to:

Industry
 Forging, the process of deforming a metal workpiece by working with localized compressive forces
 Finery forge, a works where pig iron was fined and forged (drawn out) into bars of wrought iron
 Bloomery, a forge where iron ore was smelted to produce wrought iron

Media and arts
 Forge (comics), a mutant superhero in the Marvel Comics universe who is associated with the X-Men
 Forge (Doctor Who), a fictional black operations organisation from the Big Finish Productions audio plays based on the long-running British science fiction television series Doctor Who
 Forge, a book of poetry by Jan Zwicky, 2011
docs say this:
In the case of bi-directional associations you have to update the fields on both sides:

This means that you'll have to do something like:
$second->setParent($first);

As $second table has the foreign key.
Or you could add a cascade={"persist"} option to the$first->secondList property.


The Syllis gracilis species complex: A molecular approach to a difficult taxonomic problem (Annelida, Syllidae).
Syllis gracilis is an emblematic member of the subfamily Syllinae (Syllidae, Annelida), which inhabits shallow, temperate coastal waters and can be found on algae, coral rubble, and sponges. Their distinctive ypsiloid chaetae, usually found in specimens from populations all around the world, led to the consideration of the species as cosmopolitan, even though four other species have similar chaetae: Syllis magellanica, S. picta, S. mayeri and S. ypsiloides. The discovery of deeply divergent lineages in the Mediterranean Sea, that were morphologically similar, questioned the cosmopolitanism of S. gracilis and suggested the possibility of it being a species complex. In order to assess the speciation patterns within the putative S. gracilis complex, we undertook speciesa simple device that assists hip flexion can reduce the metabolic cost of walking in older adults. The choice to design a device helping hip flexion in the elderly was decided for two main reasons. The first is that it has been established previously that aging causes an increased reliance on the hip rather than on the ankle to power walking \[[@CR17], [@CR18]\]; the second is that a relevant simulation study on powered exoskeletons \[[@CR19]\] indicated that assisting hip flexion provides greater metabolic savings with respect to other joints. Research in the exoskeleton field has also highlighted that physiological and neurological differences between individuals can cause divergent metabolic responses to the same device \[[@CR20]--[@CR22]\], and that responses can change considerably during the course of adaptation \[[@CR23], [@CR24]\], thus


underlining the importance of an individualized level of assistance applied by the device \[[@CR25], [@CR26]\]. As such, we evaluated three different levels of assistance associated with a specific force index: LOW (0.3 N/kg), MED (0.5 N/kg) and HIGH (0.7 N/kg) applied by our device in a group of healthy community dwelling older adults.

Materials and methods {#Sec2}
=====================

Exoband design {#Sec3}
--------------

A hip Exoband (Fig. [1](#Fig1){ref-type="fig"}) was fabricated. The Exoband is extremely lightweight since it is composed of textile and 3D printed parts. It is made of three main components: a waist belt (Fig. [1](#Fig1){ref-type="fig"}a) and two thigh parts, one for each limb (Fig. [1](#Fig1){ref-type="fig"}b). Each thigh part is connected to the waist belt by means of an elastic element of which the length is set by means of a ratchet strap placed in series with it. TheBaiting fears as badger is snared

Police believe a badger found trapped in a snare in Bradford was caught for the illegal sport of baiting.

The young male badger suffered serious neck injuries after it became stuck in a cruel self-locking device in a remote wooded area of Clayton.

It was found suspended from a snare with its head barely touching the floor by 21-year-old Claire Hardaker, of Tyersal, who was out on the field with her horse Brandy.

“I spotted the badger on the other side of the valley. It was quite still but it started hissing at us. I felt horrified and angry and went for help.”

Stephen Henshall, who owns the land where it was found, called Bradford pest controller Peter Highsmith to help rescue the animal whose nearby sett


had been dug up and almost destroyed.

Mr Hensall, a printer who works at Kingsley Cards in Skipton, said: “I was mortified, it is the first time in 48 years I have seen a live badger and it was only just alive.

“My wife Gill thought it was going to die. When we got it into the cage and took it back it ate three quarters of a tin of cat food and half a dozen eggs and drank lots of water.”

He added: “I think the people that did this want putting in a snare.”

Wildlife officer PC Phillip Sanderson said he believed the badger would have been used for the brutal and banned practice of baiting.

“It was caught for a specific purpose, I feel they wanted to catch it alivebackground. They also have an associated characteristic emotional state, that we could call a feeling.

Surprise

We can define surprise as the reaction provoked by something unexpected, new, or strange. In other words, it’s when a stimulus appears that the subject had not thought about beforehand. The subjective experience that accompanies surprise is a feeling of uncertainty, along with the sensation of one’s mind being completely blank.

In terms of physiological reactions, we usually see a decrease in heart rate and an increase in muscular tone. Breathing gets deeper, pitch goes up, and the subject makes spontaneous vocalizations.

The purpose of surprise is to empty the working memory of all residual activity in order to face the unexpected stimulus. To that end, surprise activates attention processes, along with behavior related to exploration


in a manner that is transparent to the application program.
It is a further object of the present invention to perform hardware emulation in a straight-forward and simple manner.
It is a still further object of the present invention to provide hardware emulation circuitry in which peripheral emulation hardware prevents the processor from accessing registers within the peripheral device controller which contain peripheral device-specific parameters.
It is yet a further object of the present invention to allow the processor to access registers in the peripheral device controller which do not contain peripheral device-specific parameters.
It is yet a further object of the present invention to provide peripheral emulation hardware in which peripheral-specific parameters in the peripheral controller are programmed by a second processor, without interfering with the operation of the primary processor.thought to originate from mucosa associated lymphoid tissue (MALT).\[[@ref3]\]

Follicular lymphomas accounts for one third of NHL.\[[@ref4]\] This is a low to intermediate grade lymphoma that show a follicular architecture and represents the neoplastic counterpart of germinal center B lymphocytes. Follicular lymphoma is very uncommon under the age-group of 40 years and is extremely rare in children. Male is to female ratio is 1.3:1.\[[@ref2]\] Somatic hyper mutations responsible for high intraclonal diversity, is characteristic of this malignancy.\[[@ref5]\]

The t (14;18) (q32;q21) chromosome translocation has been purported to be the cytogenetic hallmark of follicular lymphoma. The molecular consequence is deregulation of Bcl2 expression leading to over expression of Bcl2 proteins in neoplastic follicles.\[[@ref6]\]

Primary NHL of the salivary glands are relatively uncommon.\[[@ref3][@ref7][@ref8]\] Here we present a case of Primary NHL involving submadibular


salivary gland.

CASE REPORT {#sec1-2}
===========

A 40-year-old female patient reported to our institute with a complaint of swelling in the left side of lower jaw since 2 months \[[Figure 1](#F1){ref-type="fig"}\]. Post its onset, the swelling gradually increased in size over a period of 2 months. The swelling was associated with dull pain. The patient is a known diabetic since 5 years and is under oral anti diabetic medication. Concurrent with the history of swelling, the patient gives a history of weight loss in past 2 months. No history of cold, cough or fever.

![Clinical picture showing the swelling in the left submandibular region](JOMFP-18-163-g001){#F1}

Examination revealed a swelling of 9 × 4 cm in size which extended anteriorly 1 cm in close proximity to chin on left side and posteriorly in line withnearby areas.

Petrobras has discovered around four times the crude that it was authorized to produce as per its Transfer of rights agreement. Hence, the company will be required to pay 2 billion Brazilian reais in 2014 as advance payment for the expansion of production rights. It will have to pay an additional 13 billion Brazilian reais in the next four years based on the anticipated yield.

Production sharing agreements for the additional produce will come into effect from the date of first production from each of the four regions of Búzios, Entorno de Iara, Florim and Nordeste de Tupi. As per reports, production from this expansion is expected to begin in 2020 or 2021. Petrobras believes that considering the parameters set for this production rights expansion, the project will


levels fell rapidly by 85% within 24 h following parturition. By in situ hybridization, OTR mRNA was localized specifically to the longitudinal and circular layers of the myometrium but was not detected in the endometrium. During the estrous cycle, OTR mRNA levels increased 2-fold between metestrus and proestrus, whereas oxytocin (OT) binding rose more than 10-fold within this same interval. Treatment of ovariectomized rats with E2 lead to a significant increase in both OTR mRNA levels (4.4-fold) and OT binding (< 6-fold). Cotreatment with P4 strongly reduced OT binding by 75% (P < 0.01) but did not significantly affect the E2-induced rise in OTR mRNA (11% decrease, P > 0.1). Our data suggest that the increased expression of OT binding sites observed at the onset of labor andat proestrus is mediated, at least in part, by an E2-induced up-regulation of OTR gene expression. However, it also appears that OTR mRNA levels are not the sole determinants of uterine OT binding. Specifically, P4-mediated OTR down-regulation cannot be explained by an effect on OTR mRNA accumulation and may involve novel mechanisms acting at translational or posttranslational levels.


with details one who wants to draw armor for the Mane Six would not come up with almost instantly, but only after a long time of experimenting with the design, and if this design would get copied by another artist, then there would be evidence that is impossible to refute.

But with a design that easy, obvious and simple? No one will copy it and no artist has to.

Don't get me wrong, Equestria-Prevails' design looks great, but it's not creative or original enough that another artist can't get the same design ideas. In fact, due to its simplicity and blatancy, it's very likely that another artist ends up with the same design at some point.

It's just because it's Jay Fosgitt who happened to be that artist that so manyGenetic derangements in the tumor suppressor gene PTEN in endometrial precancers as prognostic markers for cancer development: a population-based study from northern Norway with long-term follow-up.
The purpose of the current study was to characterize the role of PTEN in malignant transformation and to evaluate the significance of mutated PTEN exons as prognostic markers in the carcinogenesis of endometrial hyperplasia. A comparison of PTEN mutations as prognostic markers with former investigated prognosticators was also intended. Histological material from 68 patients with endometrial hyperplasia and 10-20 years of follow-up of whom 18 later developed cancer was examined. PCR amplification and DNA sequencing were performed, screening the most frequently mutated exons 5a-8b of the PTEN gene. Mutations were demonstrated in 13.2% of the patients. Of the patients with cancer development, five


C}=205.3$K) and similar maps were recorded also at 0.3GPa and temperatures 267, 270, 273, and276 K ($T_{\rm
C}=271$K). However, no obvious satellite peaks have been seen.

![(Color online) Zero-energy-transfer scans in the $(\bar{2}00)$ Brillouin zone of the Sn$_2$P$_2$S$_6$ crystal taken in the vicinity of the ferroelectric phase transition (at 0.6GPa; near $T_{\rm C} = 205.3$K). (a) Scans in the $ [-0.03,0,1]$ direction (approximately along the diffuse streak), and (b) scans in the $[1,0,0.05]$ direction (across the diffuse streak). Measured scan trajectories are schematically indicated in the insets. Solid lines are just guides for the eye.[]{data-label="newLabelF4"}](Fig4Ondrejko.eps){width="8cm"}

![(Color online) Schema of reciprocal-space trajectories of data scans shown in Fig.\[newLabelF4\], superposed with contour plots of the critical diffuse scattering known from ambient pressure x-ray diffraction measurements of Ref.. Full triangles indicate positions of incommensuratein the dielectric permittivity data (Ref., triangles in Fig.\[newLabelF7\]). Both these measurements suggest splitting of the phase transition in Sn$_2$P$_2$S$_6$ ($x=0$) at around $p=0.18$GPa, $T=296$K, which was naturally interpreted as the expected LP. At the same time, the stability range of the anticipated intermediate phase is markedly different in these two measurements: It opens at the rate of about $190$K/GPa according to Ref. (“scenario B”), while there is only about $20$K/GPa opening rate deduced from measurements of Ref. (“scenario C”).

![(Color online) Temperature-pressure phase diagram of Sn$_2$P$_2$(Se$_x$S$_{1- x}$)$_6$ solid solution.[@Tyagur; @Sli99; @Sli90; @Slivka05; @Barsamian86] The shaded areas indicate intermediate phases between high-temperature paraelectric and low-temperature ferroelectric phases for given concentrations $x$. The stars stand for Lifshitz points of solid solution with $x$ equals: 1, 0.28, 0.2 0.04, and 0


are looking for decorative barriers made from cast iron or stainless steel. Some serve double duty as bicycle racks.

It's this market that Reliance is homing in on. Bollards with decorative metal covers help disguise the beefy metal and concrete barriers beneath.

"We ship product into every state in the U.S. and every province and territory in Canada," he said on a tour of his warehouse.

Bollards erected on sidewalks and plazas protect pedestrians in Times Square, New York City. (Mike Segar/Reuters)

"We have product in front of the Federal Reserve, Apple headquarters, Cirque du Soleil, major hotel chains, major restaurants, just about everything."

His company alone is now selling about 15,000 bollards a year.

Attacks using vehicles on the rise

Worldwide, the number of deliberate attacks using vehicles against pedestrians has been on the"Previously on "Alias:"" "Something you should know." "I spent three years studying you for my postdoctorate dissertation." "And to some degree I understand you, Arvin." "And I can help you if you let me try." "I lie." "I keep secrets." "I divulge only what I must in order to elicit the reaction I need." "One of those secrets affects the only two people I care about in the world." "Sydney and Jack Bristow." "This watch belonged to my father." "It's broken now, but it used to keep perfect time." "He said,"You could set your heart by this watch. "" "It stopped October 1, the day we met." "We will find each other." "We always find each other." "You two are gonna continue working together." "You're gonna be heading


lease vault space." "Once inside you'll uplink with Marshall." "Now, if we're lucky, the operative will have archived the details of the shipment and we can intercept the bomb before it's delivered." "Shall I tell them the idea I had?" "I had a great idea what you guys should..." "I think you should pose as radio astronomers." "You know, looking to back up your recordings of interplanetary noise." "Now, I came up with a little cheat sheet that should help you, and you can review this en route." "And one more thing." "The Covenant may have intercepted CIA intelligence regarding our mission in North Korea and our pursuit of the Doleac agenda." "Langley has assigned a counterintelligence team to determine whether someone within the agency has been leaking"It has to be." "There's no other way to access the files." "I think they just planted a virus." "It has to be the Covenant." "The mole must have leaked our intel." "They beat us here." "You guys, listen to me." "Disconnect from their system before their virus affects our copy." " Did we get the shipping information?" " I'm not sure." "I'll have to see if I can reconstruct the data from what we've got." "Let's get out of here." "Please." "Don't do this." "The shooter." "There!" "Vaughn!" "The F-150." "Vaughn!" "How much of the data did we get our hands on?" "Looks like 63 percent, give or take a few megs." "Oh, I slept 20 minutes last night, FYI." "How long before you can extrapolate the


the silicon nitride layer 102 on the top of the stacks 51, 52 and 53 is clearly exposed. The exposed portion of the nitride layer 102 is then removed. After the exposed nitride layer 102 is removed, the remaining photoresist layer 101 is hardened and the polysilicon 81 above the stacks is selectively removed. The remaining photoresist material 101 is then removed. The resulting structure is depicted in FIG. 5B.
In the above process, it is important that only those portions of the nitride layer 102 and the polysilicon layer 81 directly above the stacks be removed. Therefore, it is important that the photoresist be of uniform thickness over the stacks. Also, it is advantageous if the resist material is much thicker over the areas of the substrate betweenDNA copy number changes, including increases at 1p22-32, 2p24-25, and 3q22-25 and a decrease on 18p, were found to occur preferentially in small cell lung carcinoma lines of the "variant" phenotype. This correlation suggests that genes may reside at these loci whose overexpression or inactivation contributes to the radiation resistance or aggressive growth phenotypes characteristic of this subtype of small cell lung carcinoma.


to clear the blockage. With the Wollet device, once the coin chute is stuffed with foreign objects, a metal plate shuts the coin deposit slot on the coin activated machine, thereby blocking further access to the coin deposit chute and rendering the coin activated machine inoperative. Although later customers are prevented from losing their coins to thieves, sales are lost until service personnel make a service call to clear the machine. U.S. Pat. No. 4,687,090 to Ramseier discloses a coin receiving mechanism having separable coin guide walls which define a coin track having sections arranged in a zigzag form. While the Ramseier device purportedly has a feature to allow unblocking of the coin chute path, one of the walls, which is set at a steep angle to theThe c.496C > T:p.Arg166X resulted in a truncated CEP112, which was verified by the mutation expression plasmid. The CEP112 expression was significantly reduced in the P2, suggesting the biallelic mutations c.2074C > T and c.2104C > T may affect the function and stability of CEP112. Therefore, we speculate that the loss-of-function mutations in CEP112 may be account for the human acephalic spermatozoa phenotype.


less patient with me when I pointed out that her support for Michael Moore's plan to specifically ban Holyrood from introducing votes at 16 for the referendum meant that she wasn't so much in favour of votes at 16 as...well, opposed to it. That was simply an example of me being "difficult", apparently, and I needed to grow up and accept that there were insurmountable logistical issues standing in the way of 'what we both wanted to happen'. Logistical issues such as Michael Moore's proposed ban, presumably.

It's a great pity that MrsB is no longer anywhere to be found, because I suspect the cognitive dissonance brought about by the events of the last few days may well have been a sight to behold. A UK government spin doctora 12:12 hour light:dark cycle with food and water *ad libitum*. Mice were randomly assigned to one of four conditions, sedentary (*n* = 8), sedentary with simvastatin treatment (*n* = 7), wheel running (*n* = 8), and wheel running with simvastatin treatment (*n* = 8). Sample size calculations were based on *PGC-1α* mRNA expression as it is a robust indicator of mitochondrial biogenesis \[[@pone.0172551.ref017]\]. A sample size of at least 5 per group was calculated *a priori* as sufficient to detect a 2-fold increase in *PGC-1α* mRNA expression assuming a power of 0.8 and a α-level of 0.05. All mice were assessed for treadmill running aptitude following the 6-week study, and 72-hours after the treadmill running test mice were assessed for ankle plantarflexion muscle function *in vivo*. Gastrocnemius,


Columbus, Ohio) in individual lanes. The treadmill protocol started with two stages, 7.5 m/min for 7 min and 10 m/min for 7 min followed by an increase of 2.5 m/min every 10 min until a maximum speed was achieved of 27.5 m/min \[[@pone.0172551.ref024], [@pone.0172551.ref025]\]. Percent grade remained constant throughout the test at 5%. Brushes at the end of the treadmill lane encouraged mice to keep running throughout the test. The fatigue test was terminated when mice no longer responded to 5 consecutive, forceful taps with the brushes. Upon fatigue, mice were quickly removed from treadmill lane and a post-test lactate reading was obtained. Treadmill distance and time were recorded for all mice.

*In vivo* muscle function {#sec007}
-------------------------

*In vivo* peak isometric torque of the ankle plantarflexors was assessed as previouslysynthase (CS), β-hydroxy acyl-CoA dehydrogenase (β-HAD) activities were determined in triplicate as previously described \[[@pone.0172551.ref031]\]. Enzyme activities were normalized to total protein content (BCA; Thermo Fisher Scientific, Grand Island, New York).

For isolated mitochondrial respiration, mitochondria from tibialis anterior muscles were isolated and assessed for state 4, state 3, and state 3 uncoupled oxygen consumption according to the protocol outlined by Garcia-Cazarin et al. \[[@pone.0172551.ref032]\]. Briefly, mitochondria were isolated using a series of centrifugation steps performed at 0--4°C and resuspended in a final 100 μL of isolation buffer \[[@pone.0172551.ref032]\]. Protein concentration of the mitochondrial homogenate was determined via BCA assay. All measurements were performed using a Clark-type electrode (Oxygraph Plus System, Hansatech Instruments, UK) at 25°C. Prior to each experiment, the electrode was calibrated according to the manufacturer's instructions


indirect measurement of skeletal muscle exercise adaptations. To directly test the impact of simvastatin treatment on skeletal muscle contractile adaptations to endurance exercise training, we next performed *in vivo* contractile assessment of the ankle plantarflexors.

![The effects of exercise training and simvastatin treatment on treadmill running capacity and contractile fatigue resistance (n = 7--8 per exercise/treatment group).\
*A*: Distance run during a progressive velocity treadmill running test to exhaustion. Run \> Sed indicates main effect of training, *P* \< 0.001. *B*: Plantarflexor torque loss following a fatiguing bout of 120 contractions. Run \> Sed indicates main effect of training, *P* \< 0.040. Torque loss is expressed as a percent of the peak torque generated during the protocol.](pone.0172551.g001){#pone.0172551.g001}

*In vivo* muscle function {#sec015}
-------------------------

Trained mice had significantly less plantarflexion torque loss during a*COXIV* gene expression, which are reliable and well-established markers of mitochondrial biogenesis \[[@pone.0172551.ref017]\]. Trained mice had an \~2-fold greater *PGC-1α* expression (*P* \< 0.003, [Fig 3A](#pone.0172551.g003){ref-type="fig"}) and \~3-fold greater *COXIV* expression compared to sedentary mice, independent of simvastatin treatment (*P* \< 0.001, [Fig 3A](#pone.0172551.g003){ref-type="fig"}). We then proceeded to assess vascular gene responses to training and simvastatin treatment, as adaptations to the vascular network would be required to meet the demands of a larger mitochondria network. There was no effect of simvastatin treatment on vascular gene responses to endurance exercise training, and trained mice had a greater *CDH5* (*P \<* 0.007, [Fig 3B](#pone.0172551.g003){ref-type="fig"}), *CD31* (*P* \< 0.002, [Fig 3B](#pone.0172551.g003){ref-type="fig"}), and *NOS3* (*P* = 0.046, [Fig 3B](#pone.0172551.g003){ref-type="fig"}) expression compared to sedentary mice.

![The effects of exercise training and simvastatin treatment on


       44.2 ± 11.7    49.9 ± 14.1[\*](#t001fn002){ref-type="table-fn"}   49.1 ± 4.87   50.1 ± 13.6                                         0.973       0.821      0.705
  State 3 Uncoupled                               80.7 ± 19.0    71.5 ± 27.5[\*](#t001fn002){ref-type="table-fn"}   67.6 ± 4.62In accordance with previous findings \[[@pone.0172551.ref045]\], we found exercise trained mice had greater levels of the autophagy-related proteins necessary for autophagosome formation and expansion ([Fig 4A](#pone.0172551.g004){ref-type="fig"}). However, we also found that simvastatin treated mice had lower levels of Drp1 ([Fig 5B](#pone.0172551.g005){ref-type="fig"}), which is an essential protein for mitochondrial fission during autophagy. Mitochondrial fission is the structural separation of mitochondria from the mitochondrial network, a critical step for the successful removal of damaged or dysfunctional mitochondria during autophagy. Long-term reductions in mitochondrial fission as the result of a chronic statin use could potentially have negative consequences on the function of the mitochondrial network and basal autophagy ([Fig 6](#pone.0172551.g006){ref-type="fig"}). In line with this hypothesis, IIkeda et al. used a conditional Drp1 knockout mouse and shRNA to downregulate Drp1 *in vivo*


own analysis of pUlk1, Beclin1, Bnip3, and p62 protein contents ([Fig 4A](#pone.0172551.g004){ref-type="fig"}). However, the LC3-II protein content in simvastatin treated mice are harder to interpret given that LC3-II accumulation is present in both sedentary and exercise trained mice ([Fig 4C](#pone.0172551.g004){ref-type="fig"}). When the LC3-II data is interpreted along with the lower Drp1 protein levels in simvastatin treated mice, one could reasonably consider that simvastatin treatment potentially results in impairment in autophagosome elongation and maturation (i.e., autophagy inhibition). We are not the first to report LC3-II accumulation with simvastatin treatment \[[@pone.0172551.ref048]\], but our comprehensive autophagy-related protein analysis suggests more research is necessary in order to determine the long-term benefit/detriments of statins on skeletal muscle autophagy, especially when considering the efficacy of statin treatment alongside comorbidities such as muscular dystrophy, aging,Presence of metabolic syndrome and cardiovascular risk factors in adolescents and University students in Crete (Greece), according to different levels of snack consumption.
The aim of this study was to investigate the presence of cardiovascular and metabolic syndrome (MetSyn) risk factors in adolescents and University students in Crete (Greece), according to different levels of snack consumption. 237 adolescents (14.2-16.3 years) and 196 University students (19.5-31.5 years) participated during 2002/3 and 2004/5. Dietary intake (24-h dietary recalls), cardiorespiratory fitness, smoking habits, time spent watching television, anthropometric, blood pressure and biochemical measurements were assessed in all subjects. Adolescents consumed snacks more often than University students (62% vs. 49.5%, P < 0.05) and had significantly higher total energy intake (8629 vs. 7641 kJ, P < 0.05) and energy intake from snacks (2537


(7.0 out of 10) since the great economic meltdown. Several factors in particular characterize the world’s happiest countries – a relatively small gap between rich and poor; excellent work-life balance; urban design favoring community over cars; high degrees of interpersonal trust; a strong social safety net, and, contrary to popularly-held US ideas, the highest tax rates in the world.

Putting Happiness First

Organizers of the Vermont conference hope to launch a movement that puts happiness and wellbeing at the forefront of policy ideas and educational goals. The event features more than 50 prominent speakers, including Vermont state Senator Anthony Pollina, author of the Vermont GPI legislation, Linda Wheatley and Tom Barefoot, lead organizers of GNHUSA, the Vermont organization that has been the primary conference organizer, Laura Musikanski of the Happiness(within the previous 3 months) included anorectic agents, laxatives, β-agonists (other than inhalers), diuretics, cyproheptadine, anti-depressants, anti-serotonergics, phenothiazines, barbiturates, oral corticosteroids and anti-psychotics. Women who were pregnant, breastfeeding or of childbearing potential and not taking adequate contraceptive precautions were also excluded.

Treatment {#S0002-S2003}
---------

During the run-in period, patients underwent a 1-month wash-out period during which statins were stopped. At the end of the wash-out period, if CPK and/or transaminases returned within an acceptable range (below three times the ULN) and clinical symptoms were mitigated, statins were re-introduced at half of the previously taken dose ([Tables 1](#T0001){ref-type="table"} and [2](#T0002){ref-type="table"}). After one month, patients were randomized to take either a liquid CoQ10 supplement (Q-Factor, a water-soluble CoQ10 based on micro-sphere dispersion technology; Giellepi S.p.A, Italy) or a placebo for three months using a


increase ≥3 × ULN (n)01567ALT increase ≥5 × ULN (n)00212AST increase ≥3 × ULN (n)01347AST increase ≥5 × ULN (n)00123ALT and AST increase ≥3 × ULN (n)10024ALT and AST increase ≥5 × ULN (n)00000CPK increase ≥3 × ULN (n)13898CPK increase ≥10 × ULN (n)00000Asthenia (n)12787Myalgia (n)22478Rhabdomyolysis (n)00000[^3]

Coenzyme Q10 {#S0003-S2002}
------------

Coenzyme Q10 levels were significantly higher after 3 months in the CoQ10 group, both compared to the baseline and the placebo (*p*\<0.05 for both) ([Table 4](#T0004){ref-type="table"}).Table 4Baseline, 1 and 3 months data of patients during CoQ10 supplementation and placeboParametersCoQ10PlaceboBaseline1 month3 monthsBaseline1 month3 monthsPatients (n)303029302927M/F13/1713/1713/1615/1515/1414/13Age (years)59.8±8.3----58.3±7.9----Smoking status (M/F)4/54/54/56/46/45/4Height (cm)1.70±0.05----1.69±0.04----Weight (Kg)78.6±5.178.2±4.978.4±5.078.7±5.478.9±5.678.5±5.0BMI (Kg/m^2^)27.2±1.827.1±1.727.2±1.827.5±2.027.6±2.127.4±1.9Waist circ. (cm)89.1±2.789.0±2.688.9±2.489.3±2.989.2±2.789.0±2.6Hip circ. (cm)86.4±2.286.3±2.186.1±2.086.8±2.586.7±2.086.5±1.9Abd. circ. (cm)98.3±3.698.0±3.497.2±3.099.5±3.999.3±3.799.1±3.5CoQ10 (μg/l)759.4±93.8802.7±102.6875.1±143.5\*\^764.1±95.7757.6±91.9748.1±88.2TC (mg/dl)196.4±21.4192.5±20.2\^188.3±17.6\^°199.3±22.2215.7±24.9\*232.7±28.1\*\*LDL-C (mg/dl)127.5±11.8121.9±9.7\^116.7±9.1°129.8±12.4144.9±19.1\*162.1±25.7\*\*HDL-C (mg/dl)44.1±3.944.4±4.244.6±4.343.8±3.744.1±3.943.7±3.6Tg (mg/dl)124.2±25.8130.8±28.3135.1±31.2128.3±27.5133.4±28.9134.7±29.2AST (IU/l)68.1±57.255.7±43.241.9±28.1\*\^65.9±53.160.8±50.254.3±39.5ALT (IU/l)74.5±52.167.9±48.452.7±41.3\*\^70.3±50.264.2±47.158.8±42.9Creatinine (mg/dl)0.9±0.40.9±0.40.8±0.31.0±0.50.9±0.41.0±0.5CPK (IU/l)528.6±113.7499.2±93.9265.1±41.4\*\^524.2±111.9419.5±109.8398.6±90.7VAS score5.3±2.44.8±2.13.1±1.2\*°5.6±2.75.2±2.54.9±2.2Clinical index score10.3±2.18.9±1.87.3±1.6\*°10.9±2.610.5±2.39.6±2.0[^4][^5]

Clinical symptoms {#S0003-S2003}
-----------------

The Clinical Index Score for myalgia assessment was lower after 3 months with CoQ10 (7.3±1.6 vs 10.3±2.1 atbeen described by other authors.[@CIT0031] Our results confirm the lipid-lowering action of CoQ10, which enabled us to reach the therapeutic target for LDL cholesterol in a higher percentage of patients treated with the dietary supplement in comparison to the placebo group at half dosage of statin. The administration of CoQ10 in patients with intolerance to statins is able to both improve the quality of life (QoL) and reduce the cardiovascular risk. In fact, in addition to the beneficial effects on the liver and lipid parameters, CoQ10 administration could improve myocardial energy metabolism and decrease physical exertion, as has been shown for patients with chronic heart insufficiency.[@CIT0032],[@CIT0033] Moreover, a great number of intolerant patients interrupt the lipid-lowering treatment and/or significantly reduce physical activity because of fatigue and myalgia. This


leads to a significant increase in adverse cardiovascular events.[@CIT0034],[@CIT0035] Such a situation could be reduced with CoQ10 supplementation, which should also increase the persistence of statin therapy.

Differently from other previous studies,[@CIT0029] we found a greater reduction in CPK levels after CoQ10 supplementation and half dosage of statin. This result is very interesting, although the plasma levels of CPK are not considered sensitivity markers for statin myopathy.

At first sight, the results of our study appear to suggest that CoQ10 has a neutral effect on the lipid profile, but this is not true. It is important to remember that in our study, patients were taking a half dosage of statin at baseline, so a worsening of the lipid profile would be expected. This happened in the placebo group, but itRubbermaid Smokers Pole is a simple, space saving solution that controls smokers` waste. Easy-to-empty ergonomic design that requires no tools and minimum bending. Features a weather resistant powder coated fire safe aluminum finish with a recessed extinguishing screen. Self extinguishing. No sand or water needed. Top of the unit has a 4 inch Dia. Base is weighted for stability.

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At 11:10 a.m. Tuesday, Billups addressed jurors for about 3o minutes before the court recessed for lunch. Billups and Levy continued their arguments in the afternoon for about an hour before handing the floor back to Brouhard — as required by law — for a final rebuttal.

Billups, right off the bat, asked the jury to remember that it’s Brouhard who has the burden of proof to make his case due to the presumption of innocence, and said there was plenty of room for reasonable doubt: “If you’re not convinced then you should return verdicts of not guilty.”

He focused his morning remarks mostly on the Medearis killing, trying to make the case that there had been no robbery, or even an attempt at one, based on the testimony inkilled Williams’ good friend, Jermaine Davis, in the hours before the Oakland shooting. York’s family — his children and their mother — lived in the Wilson Avenue apartment, though he was no longer in touch with them.

Billups said Davis’ cousin, Joe Carroll, actually confronted York that same night at a gas station in Antioch. If the motive was to retaliate, and if someone else was actually confronting York, what motive would there be to show up on Wilson Avenue, Billups asked. Both attorneys said authorities dropped the ball in looking for other people who may have been upset about Davis’ death who could have been responsible for the shooting on Wilson.

Police did not speak to either Nuño or Rogers until months after Alaysha’s death. Billups said that was


a problem, because details about the shooting could have made their way out into the community through another adult — Fields’ son — who was at the Wilson Avenue apartment during the shooting but was not injured. He could have told people, for example, that Fields had been shot while she was on the couch, a detail Brouhard said would have been known only to the killer.

“People are talking about this case. The streets are talking,” Billups said. “Word gets around. Things get out.”

He also played a phone call between Williams and Rogers, from Sept. 7, 2013, to undermine her assertion that she was terrified of Williams and — further — to diminish her credibility. In the phone call, she and Williams are flirtatious, and she expresses interestOn the basis of existing evidence, the most likely model is that the Rad1--Rad10 endonuclease functions downstream of Rad52, cleaving nonhomologous DNA ends prior to gap filling and DNA ligation of the recombination site. However, the precise timing of Rad1--Rad10 recruitment to DSB sites relative to other DSBR proteins has not been directly demonstrated. Additionally, recent work has revealed that Saw1 recruits the Rad1--Rad10 complex to DSBs in the context of single-strand annealing (SSA), one mode of DSB repair ([@B13]). Other than for SSA, the mechanism by which Rad1--Rad10 is recruited to DSBs remains largely unclear ([@B13]).

Recent studies have demonstrated the utility of fluorescence microscopy in monitoring biochemical events in live yeast cells ([@B5],[@B14]). Three advantages of this experimental approach are especially noteworthy. First, the gene that encodes


in this study were prepared by genetic crosses. The *rad51Δ* and *rad52Δ* genotypes were confirmed by gamma-irradiation (400 Gy) sensitivity.

UV survival studies
-------------------

The UV sensitivity of the Rad10-YFP strain was tested using standard methodologies in parallel with isogenic wild-type and deletion mutant controls ([@B16]). Triplicate experiments were conducted and the results averaged and reported along with the corresponding standard error.

SSA/gene conversion assays
--------------------------

SSA/gene conversion (GC) assays were conducted essentially as previously described ([@B17],[@B18]). Strains were co-transformed with the pJF6 reporter plasmid and a plasmid containing the *HO* endonuclease under the control of the *GAL* promoter, pFH800. Transformants were cultured in YEP-Lactate to a cell density of 5 × 10^6^ to 1 × 10^7^ cells/ml, induced by addition of 1/10 volume of 20% aqueous galactose (w/w) and incubated with shaking (2--4the DSBs. Yeast strains (PF025-7A, WPF021-4A, PF030-49A and PF023-15A) containing the Rad10-YFP/DSB-RFP labeling system were transformed with plasmid pWJ1320, and cultures of transformants induced with galactose as described in 'Materials and Methods' section. YFP and RFP foci in fluorescent images from the resulting cells were counted. Black bars show results corresponding to cells following induction with galactose. White bars show results corresponding to uninduced cells. Error bars indicate the standard error from three independent experiments in each of which at least 100 cells were counted and classified. Data corresponding to Rad10-YFP/DSB-RFP colocalized foci are shown for wild-type experiments alongside those conducted in isogenic strains deleted of either the *MRE11* (*mre11Δ*), *RAD52* (*rad52Δ*) or *RAD51* (*rad51Δ*) genes. Figure 5.(**A**) A strain containing Rad10-YFP, the DSB-RFP labeling system and Rad52-CFP


on glass microscope slides with DAPI mounting medium \[SC + ade, 1% \[w/v\] low-melting agarose (Fischer), 50 ng/ml 4′,6-diamidino-2-phenylindole (DAPI)\]. In addition to DIC and YFP images, cells were imaged using the YFP filter set and a DAPI/Hoechst/AMCA filter set, Chroma \#31000v2, (*λ*~ex~ = 350/50, *λ*~dichroic~ = 400, *λ*~em~ = 460/50). YFP exposures were 3 s and DAPI exposures were 20 ms.

Induction of DSBs by gamma irradiation
--------------------------------------

Gamma induction experiments were conducted as described above for I-*Sce*I induction experiments except for the following experimental alterations. Strains WPF019-26C and W8757-12A which did not contain the plasmid pWJ1320 were cultured in SC + ade medium, diluted to an OD~600~ = 0.1 and grown (3 h), irradiated with gamma radiation (40 Gy, ^60^Co source), incubated (2 h) and imaged using the samelanes 2 and 4 with lanes 5--7 in [Figure 1](#F1){ref-type="fig"}A). A control lane containing GFP protein alone (lane 1) exhibits no signal with α-Rad10 antibody, and extracts from a *rad10* deletion mutation (*rad10Δ*, lane 3) exhibit only background signal of the antibody. As expected, by fluorescence microscopy, the Rad10-YFP strain exhibits yellow fluorescence in the nucleus as indicated by colocalization of the YFP signal with DAPI-stained DNA in the nucleus ([Figure 1](#F1){ref-type="fig"}B).

Next, the functionality of the Rad10-YFP protein *in vivo* was examined. A plasmid-based assay was used to test Rad10-YFP function in SSA and GC ([Figure 2](#F2){ref-type="fig"}A). Densitometric analysis of genome blot results ([Figure 2](#F2){ref-type="fig"}B) shows that the efficiencies of SSA and GC are somewhat lower (86 and 54%, respectively) in the YFP-tagged strain compared with wild-type. However,


events likely reflect the recruitment of the tagged protein to spontaneous DSBs, as previously reported for proteins of the *RAD52* epistasis group, suggesting that Rad10 is recruited to both endonuclease-induced and naturally occurring DSBs ([@B4]). Indeed, cells co-expressing Rad10-YFP and Rad52-CFP exhibited spontaneous Rad52-CFP foci that colocalized with Rad10-YFP ([Figure 6](#F6){ref-type="fig"}). Since spontaneous Rad10 foci were consistently observed at a higher frequency (70--80%) than spontaneous Rad52 foci (5--10%), many of the Rad10 foci likely reflect the involvement of Rad10 in other DNA repair pathway(s), such as NER and MMEJ.

To determine the cell cycle dependence of Rad10 foci, cells were scored not only for Rad10-YFP and DSB-RFP foci, but also for cell cycle phase (S, G2/M or G1, see 'Materials and Methods' section). S phase cells manifested the greatestpathway since a strict requirement for *RAD1* or *RAD10* in BIR has not been established ([@B28; @B29; @B30]).

To explain the *RAD52* and *RAD51* dependence we observe for Rad10 focus formation after I-*Sce*I-induced DSBs, we suggest that the breaks are repaired by SDSA ([Figure 7](#F7){ref-type="fig"}). In a dividing cell where one sister chromatid has been cleaved by I-*Sce*I and the other is still intact, SDSA would be initiated by *RAD52* and *RAD51*-dependent strand invasion. The branched structure may be recognized by Rad1--Rad10 ([Figure 7](#F7){ref-type="fig"}). Indeed, genetic evidence for the requirements of *RAD52*, *RAD51* and *RAD10* in SDSA is consistent with this notion ([@B6],[@B31],[@B32]).

Results from experiments in which triply labeled strains containing Rad10-YFP/Rad51-CFP/DSB-RFP or Rad10-YFP/Rad52-CFP/DSB-RFP were induced with I-*Sce*I show that a significant number of Rad10-YFP/DSB-RFP foci also contained either


the first week of the campaign.

Another registered Tory donor was Yoginvest Ltd, whose sole officer according to Companies House is millionaire Nick Leslau. The Evening Standard reported in 2016 that the businessman's "latest venture is Secure Income Reit, a tax-efficient property investment firm that .... owns the freeholds to a £1.6 billion portfolio" including 19 private hospitals.

Top 10 marginal seats to watch at the general election Show all 10 1 /10 Top 10 marginal seats to watch at the general election Top 10 marginal seats to watch at the general election North East Fife Currently held by the SNP with a majority of 2 Getty Top 10 marginal seats to watch at the general election Kensington Currently held by Labour with a majority of 20 Getty Top 10Q:

How many resonance structures does the oxalate ion have?

I have drawn the Lewis structure of the oxalate ion, and I see that two oxygens are double bonded to the carbons. When finding the resonance structures, I said there are 2, but the exam solutions state 4, which was confirmed by internet images such as this:

However, I see no real difference btween structures 1 and 2, and 3 and 4. Are they considered different resonance structures?

A:

Resonance structures are not real things. As also indicated by rbw, they have weight on the actual compound. Simpler example is ozone.
http://www.wou.edu/las/physci/poston/ch222/pdf/Ch08-s12-part2.pdf
From the first slide second image there;

you can see that the actual molecule is none of the "resonance" structures, and both bonds between leftmost and rightmost oxygen atoms with central oxygen atoms have


story and presumably issue a subtle jab at President-elect Donald Trump’s immigration and refugee policies, facts didn’t seem to matter — since Mary and Joseph travelled from one city to another in the same country. And it didn’t take long for Twitter to call him out on his blunder.

“You don’t Bible much do you?” one person responded to Dowd’s tweet. “Ahh, so you just morphed that story into something it wasn’t to support your point. That’s today’s journalism for you.”

[lz_third_party align=center includes=”https://twitter.com/R_Optimist/status/812638174036054016″]

“Weird when people try to fit history into their ideology,” another responded. “You can have compassion without making up facts. That is what radicals do.”

[lz_third_party align=center includes=”https://twitter.com/TCBnole/status/812689699231899649″]

“But Mary and Joseph were traveling in Israel where they were from. They were not immigrants,” another pointed out to Dowd.

[lz_third_partyGiant Bomb Makes Mario - Part 04

Oh yeah! You've got to be able to actually complete the course you create before you upload it. Maybe less goombas?

Giant Bomb comes together to show Miyamoto what's up and make their own Mario.

Mar. 24 2016

Cast: Jeff, Dan

Posted by: Jason


Lincoln's Assassination

On the evening of April 14, 1865, the Lincolns attended a performance of Our American Cousin at Ford's Theater. John Wilkes Booth entered the presidential box and shot Lincoln, mortally wounding him. Within a short time after the President was shot, Taft recorded the following:

April 14th 1/2 past 10 o'clock P.M.
O, fatal day. O, noble Victim. Treason has done its worst. The President has been Assassinated. It has just been announced at my door that he was shot a half hour ago at Fords Theatre. Is it possible? I have just come from near the scene, it is too True. 11 o'clock P.M.

The Read the detailed account of the assassination in Taft's next entry, recorded on April 30, little more than two weeks later. Here Taft gaveactors in the Murder, and attack on Mr Seward, or of complicity with the assassins" and mentioned the search for Jefferson Davis, whom Taft believed was implicated in the conspiracy to assassinate Lincoln.

The last entry in the diaries dated May 30, 1865, recounted the military achievements of the last months of the war. It concludes

…But during this period of Six or Seven months, the realy most important events have not been yet mentioned. In the midst of a desolating War, in the smoke and heat of Battle as it were, There has been a quiet Presidential Election and Inauguration, with all its usual attendant excitements. A Draft of three hundred thousand men has been ordered, and the men raised. The Government Credit has been increased at home and


nothing popping up :\ i'll check the antivirus but i don't understand how it affects league but not overwatch
EDIT: i just checked my antivirus and league is listed under the "do not disturb" apps, so it won't show any noification when the game is in fullscreen

It doesnt have to be antivirus, it can be any other program as Torrent, Skype, etc. that start working on turning on PC and work behind your back but doesnt show on screen , they can be seen in End Task/Process or next to clock down-right. Try to free PC of unnecesery things like that.

Im for Diana to get or some other AP champ, they can have beautifull efects with Project skins. But for other suggested champs im sceptic , there are alreadyMany investigators at Johns Hopkins (JHU) have urgent need for routine access to high sensitivity mass spectrometric analytical capabilities. The Basic Science departmental leadership, with the strong support of the Admin., are establishing a true open-access, state-of-the-art, mass spectrometric facility. In phase I of this plan, several major users, the Dean's office, and Biological Chemistry have collectively purchased a Perseptive MALDI-TOF DE instrument. As part of phase II, funds are requested to purchase a Micromass Q-TOF electrospray time of flight mass spectrometer. The complimentary capabilities of these two types of MS will greatly enhance progress of many varied NIH supported research programs at JHU. The unique capabilities of the Q-TOF MS are essential to many of these investigations. The MS Facility and the Q-TOF will be directed by


an Instructor level faculty member with extensive MS expertise. The MALDI-TOF will continue to be operated/supervised by an experienced full-time technician. The P.I. and co-P.I. will meet at-least weekly with the facility staff to discuss technical issues, workload, and priorities. An oversight committee comprised of the P.I., co-P.I., the Director of the JHU protein facility, and three Dept. Directors, who are also major users, will meet monthly to discuss policy and problems. Outside expert review will be sought at the end of the first year. Staff support will be maintained at at- least this level. Instrumentation will be kept on full service agreements for their useful lifespans. Use charges of $50/h for MALDI-TOF and $100/sample for Q-TOF MS-MS (includes data analysis) will be returned to the facility. The    request.setTransparent(true);
          request.setSRS("EPSG:4326");
          request.setBBox("47.75,12.98,47.86,13.12");

          GetMapResponse response = (GetMapResponse) wms.issueRequest(request);
          BufferedImage image = ImageIO.read(response.getInputStream());

         /* File outputfile = new File("saved.png");
            ImageIO.write(image, "png", outputfile); */

         // FileOutputStream img = new FileOutputStream("C:\\Users\\Edhem\\Desktop\\WMSimage.png");
        } catch (IOException e) {
          //There was an error communicating with the server


     //For example, the server is down
        } catch (ServiceException e) {
          //The server returned a ServiceException (unusual in this case)
        }

    }
}

A:

You need to check the contentType of the returned response and make a decision based on that value. Something like:
try {
  GetMapResponse response = wms.issueRequest(getMapRequest);
  if (response.getContentType().equalsIgnoreCase(format)) {
    BufferedImage image = ImageIO.read(response.getInputStream());
    return image;
  } else {
    StringWriter writer = new StringWriter();
    IOUtils.copy(response.getInputStream(), writer);
    String error = writer.toString();
    System.out.println(error);
    return null;
Q:

iOS7 Auto Layout, View Resize and iAds

I'm using Auto Layout in my iOS 7 project with the following view hierarchy
Main View
-Container View
---Button
---Button
---ImageView
-Banner View (iAd Banner View)
The Main View and Container View are full width and height of screen. I have Horizontal and Vertical Space Constraints on the Container View sticking to the main view (screen's height and width). And also the subviews of Container View are constrained to the button of the view with a 20px space.
My issue occurs when the Banner View is finally filled and placed at the bottom of the screen, which then I have the Container View subtract the Banner View's Height from its frame height to allow space for the Banner View to show. (code used below)  The ideal outcome is the


for any help out there.
Update:
Here is working solution for me.
Instead of default Deserializer used by RestSharp, I used Newtonsoft.Json instead.   Here is code example from my wrapper class:
 public T Execute<T>(RestRequest request) where T : new()
 {
        if (request.Method == Method.POST)
            client.FollowRedirects = false;

        var response = client.Execute<T>(request);

        // **** Added Newtonsoft.Json ****
        var x = JsonConvert.DeserializeObject<T>(response.Content);

        LogRequest(request, client);
        LogResponse<T>(response);

        ErrorHandling<T>(response);

     Q:

How to satisfy no-margin inside a NSTableCellView using Cocoa Auto Layout

I have created a custom NSTableCellView with some subviews aligned using Cocoa Auto Layout. Nothing bad at all except when I placed the NSOutlineView (using the custom NSTableCellView) inside a NSSplitView.
The problem occurs when I have tried to collapse the splitView where the custom NSTableCellView fails all the time to satisfy the horizontal constraint, in details the margin specified by the "-" character between _imageView and _textField does not to be zero length.
The horizontal constraint looks like:
[self addConstraints:[NSLayoutConstraint constraintsWithVisualFormat:@"H:|[_imageView]-[_textField]|"


Postsurgical adjuvant chemotherapy with or without radiotherapy in women with breast cancer and positive axillary nodes: a South-Eastern Cancer Study Group (SEG) Trial.
In a prospective study of 622 women with breast cancer, those with one to three histologically positive axillary lymph nodes were randomised after mastectomy to receive cyclophosphamide 100 mg/m2 orally on days 1-14, methotrexate 40 mg/m2 intravenously on days 1 and 8, and fluorouracil 600 mg/m2 intravenously on days 1 and 8 every 28 days for six cycles (CMF x six), or for twelve cycles of the same chemotherapy (CMF x 12). Those with > or = four positive nodes were randomised to one of these two groups or to 5000 cGy of postmastectomy regional radiotherapy (RT) followed by six cycles of the same chemotherapy (RTparser to get the JsonObject.
        String json3 = "{\"type\":1,\"password\":\"ac@123\",\"role\":\"normaluser\",\"name\":\"Archana Chatterjee\",\"username\":\"a.chatterjee\",\"designation\":\"Teacher\",\"id\":\"T_02\",\"age\":42}";
        JsonParser jParser = new JsonParser();
        JsonObject jObject = (JsonObject) jParser.parse(json3);

Now JsonObject has capability of returning the value of a key. javadoc for same
        JsonElement elem = jObject.get("type");

JsoElement's toString() returns the value of the key. (i.e value of type here)
        System.out.println(elem);

Output
1


It was just last month that I found myself writing about an upcoming collaboration between UNIQLO and Mobile Suit Gundam to celebrate the legendary series’ 40th anniversary. From the collaboration, we saw a number of extremely choice pieces. With that in mind, I was pretty excited when I was clued in for the announcement that they’d be coming to North America both online and in-store from March 25, 2019.

I’m totally stoked on the fact that this release is dropping outside of just Japan, for more than a few reasons, but there are still a few things that are a little unclear. Will the sweet little Gunpla kits that come with purchases in Japan also be making their way to North America? This might be a bit of aNoninvasive simultaneous assessment of wall shear rate and wall distension in carotid arteries.
A novel technique has been developed for the noninvasive real-time simultaneous assessment of both blood velocity profile and wall displacements in human arteries. The novel technique is based on the use of two ultrasound beams, one set at optimal angle for wall motion measurements and the other for blood velocity profile measurements. The technique was implemented on a linear array probe divided into two subapertures. A modified commercial ultrasound machine and a custom PC board based on a high-speed digital signal processor was used to process the quadrature demodulated echo signals and display results in realtime. Flow phantom experiments demonstrated the validity of the technique, providing wall shear rate (WSR) estimates within 10% of the theoretical