in the intervention and the causal processes targeted by the change techniques to achieve particular outcomes (theory, underlying mechanism), mode of delivery (intensity, duration, timing), intervention content (knowledge generated or shared), participants and their role (the characteristics of those sponsoring, delivering and receiving the intervention), setting and adherence or fidelity. Also, research is needed to identify, describe and test relevant theory by which to optimally design and/or evaluate PMIs.[27](#hex12653-bib-0027){ref-type="ref"}

In all studies that described patient interventions, the intervention was educational material, which achieved positive impact in only one of those studies.[23](#hex12653-bib-0023){ref-type="ref"} Patient educational material is inconsistently effective, and many other types of interventions are available to inform, educate and activate patients.[28](#hex12653-bib-0028){ref-type="ref"}, [29](#hex12653-bib-0029){ref-type="ref"} Future research should establish which types of patient interventions are most effective in different contexts. For example,your child and from being jerked around in the vehicle.

Your car seat’s plastic can become less stable over time due to exposure of extreme temperature lows and highs.

Also, you should know that the expiration date isn’t the only qualifier for getting a new car seat. If you’ve been in any type of car accident, the safety of the car seat may have been compromised, and you’ll need to replace it.

A Message from My Childcare Academy

We love your children and enjoy seeing their bright, smiling faces when they walk through our doors. Because we care for them as we would our own, their safety is our top priority. We hope this checklist will aid you in your own efforts of caring for their well-being.

Would you like to discuss this


the parties to try to talk out their differences. Those differences primarily hinge on whether the Sacklers are offering enough of their allegedly ill-gotten fortune to address the opioid crisis in thousands of lawsuits on the matter.

Purdue filed for bankruptcy in September as part of a tentative deal to settle more than 2,000 of them, which were mostly brought by state and local governments. According to Purdue's lawyers, the offered deal would be worth a total of $10 billion to $12 billion over time. Included in those figures is at least $3 billion from the Sacklers directly.

While hundreds of municipal governments and some states have agreed to the deal, about two dozen states and other plaintiffs are opposed. They argue that the Sacklers should pony up more of"The eggs in the rookery will soon hatch." "We will watch over them as if they were our own." "Why did we steal these things?" "For that matter, why are we working together?" "(roars)" "(Hudson) What a glorious night." "Aren't you coming, Goliath?" "No." "I've been looking forward to finishing this book for nights." "(Bronx whimpers)" " Not tonight, boy." "You stay here." " (whimpers)" "Hey, old man." "What's with the tin tuxedo?" "I have no quarrel with you, sirrah." "I am on a quest." "Nice shiv, tin man." "Why don't you give it to me?" "Because, young man, it is mine." "You had your chance, old-timer." "(grunts)" " Oh, man." " Who is this guy?" " I'm outta here, man." " Your leader is all noise, like


actually Tom..." " Bronx seems to like him." "Come, or the Archmage will destroy the eggs." "The Archmage?" "But how?" "Very well, then." "I will go with you." "Go where?" "The other side of the lake?" "Wait for me." "Vocati venti fortunate ex ricae Oberonis et hic navis frugum regate ad orae Avalonis." "How did you get here from Goliath's time?" "My journey has been a long and strange one." "(sighs)" " Be there any more?" " This is the last one, Your Highness." " How many were there, Tom?" " I counted 36, Mama." "I wonder how many of them will survive the journey to your Uncle Kenneth's kingdom." "All of them, Mary." "I'll see to that." "Aye!" "We'll fight off any who would threaten these eggs."my days." "(all) Hail, King Constantine." "I pledge my loyalty to the crown." "And to your own crown, when you are my queen." "(gasps)" "The princess and I shall wed on Michaelmas when I return from the inspection of my holdings." "I will never submit to this outrage!" "You have 36 very good reasons to obey me." "You're not going to marry that murderer, are you?" "Never." "But we need time to get the eggs to safety somehow." "I've been looking through the Grimorum." "I think I have an idea." "Per iussum meum adsumate similitudo ut optare." "I'll take these to the boathouse." "The guards will think it's more old kitchen stuff to be dumped into the sea." "Per iussum meum adsumate similitudo ut optare." "Taking the bairns


between job demands and emotional exhaustion. Low sensory threshold also strengthened the job resources-helping behaviour relationship. This study offered first evidence on the greater susceptibility among highly sensitive persons to the work environment and demonstrated that the moderating role might differ for the three components of sensory-processing sensitivity. Additionally, it adds sensory-processing sensitivity to the Job Demands-Resources model and highlights the idea that personal factors may act both as a personal vulnerability factor and a personal resource, depending on the nature of the perceived work environment.import unittest
from os import path
from mailmerge import MailMerge

class Issue64Test(unittest.TestCase):
    def test(self):
        with MailMerge(path.join(path.dirname(__file__), 'test_issue8.docx')) as document:
            document.merge(testfield=10)


> .btn {
      float: none;
    }
  }

  > .btn + .btn,
  > .btn + .btn-group,
  > .btn-group + .btn,
  > .btn-group + .btn-group {
    margin-top: -1px;
    margin-left: 0;
  }
}

.btn-group-vertical > .btn {
  &:not(:first-child):not(:last-child) {
    border-radius: 0;
  }
  &:first-child:not(:last-child) {
    .border-top-radius(@btn-border-radius-base);
    .border-bottom-radius(0);
  }
  &:last-child:not(:first-child) {
    .border-top-radius(0);
    .border-bottom-radius(@btn-border-radius-base);
  }
}
.btn-group-vertical > .btn-group:not(:first-child):not(:last-child) > .btn {
  border-radius: 0;
}
.btn-group-vertical > .btn-group:first-child:not(:last-child) {
  > .btn:last-child,
  > .dropdown-toggle {
    .border-bottom-radius(0);
  }
}
.btn-group-vertical > .btn-group:last-child:not(:first-child) > .btn:first-child {
  .border-top-radius(0);
}

// Justified button groups
// ----------------------

.btn-group-justified {
  display: table;
 of all of the storytelling heavy lifting themselves, and then hiring the foreign studios to pump out whatever they had in mind. It's impossible to attract Japanese creative talent to a project like that. At best, you get something like Highlander: The Search for Vengeance. There is so much anime work going on right now that no director will be all that interested in slaving away on a film they are basically making as a paint-by-numbers exercise from someone else's grand vision. In one case, the anime studio quietly subcontracted an entire job to Korea, rather than work on it themselves!

So in order for a project with Netflix to get started, a Japanese producer would basically have to pitch Netflix at an early stage of planning, and then


span multiple pages.

int ogg_stream_flush(ogg_stream_state *os, ogg_page *og);

Parameters

os

Pointer to a previously declared ogg_stream_state struct, which represents the current logical bitstream.

og

Pointer to a page of data.  The remaining packets in the stream will be placed into this page, if any remain.

Return Values

0 means that all packet data has already been flushed into pages, and there are no packets to put into the page.  0 is also returned in the case of an ogg_stream_state that has been cleared explicitly or implicitly due to an internal error.

Nonzero means that remaining packets have successfully been flushed into the page.

copyright © 2000-2013 Xiph.Org

Ogg Container Format

libogg documentation

libogg release 1.3.1 - 20130512

- 9*o(h).
-h**3 + 91*h
Let c(j) = 180*j + 4. Let p(i) = 538*i + 11. Calculate 8*c(z) - 3*p(z).
-174*z - 1
Suppose 0 = -2*y + 4*h - 16, -4*h - h = -3*y - 22. Let u(b) = -14*b**2 - 3. Let k(w) be the first derivative of -5*w**3/3 - w + 206. Calculate y*u(n) + 11*k(n).
n**2 + 1
Let d(o) = -2*o**2 - 6*o + 7. Suppose 4*a = 3*a - 8. Let n(y) = 2*y + 5. Let j be n(a). Let b(r) = -5*r**2 - 17*r + 20. Calculate j*d(f) + 4*b(f).
2*f**2 - 2*f + 3
Suppose 0 = 2*z - 6 + 2. Let n(x) = -4*x - 3. Let u(s) be the first derivative of 5*s**2/2 + 4*s - 330. Calculate z*u(o) + 3*n(o).
-2*o -


1
Suppose -6*n = -10*n - 8. Let q(j) = j + 15. Let c(u) = 4. Let w(g) = 1. Let b(p) = c(p) - w(p). Let o be 3 + (-21)/(-42) + 30/4. Give n*q(s) + o*b(s).
-2*s + 3
Let d(w) be the first derivative of 4*w**2 + 3*w - 92. Let a(t) = 7*t + 2. What is 6*a(b) - 5*d(b)?
2*b - 3
Let n(d) = 14*d**2 - d + 8. Let a be (32/(-6))/((-14)/21). Let s(c) = -9*c**2 + c - 5. Calculate a*s(x) + 5*n(x).
-2*x**2 + 3*x
Let z(q) = -11*q**2 + 10*q - 5. Let n(v) = 23*v**2 - 19*v + 11. Give -4*n(p) - 9*z(p).
7*p**2 - 14*p + 1
Let x(c) = -116. Let w(k) = k + 1. Give 2*w(o) + x(o).
2*o - 114
Let i(d)Q:

After changing the theme to MaterialComponent default EditTextPreference buttons now with white text

My styles.xml
<style name="AppTheme" parent="Theme.MaterialComponents.DayNight"><!-- MaterialComponents theme -->
    <item name="colorPrimary">@color/colorPrimary</item>
    <item name="colorPrimaryDark">@color/colorPrimaryDark</item>
    <item name="colorAccent">@color/colorAccent</item>
</style>

My preferences.xml
<PreferenceScreen xmlns:app="http://schemas.android.com/apk/res-auto">
    <!-- Default EditTextPreference created by default -->
    <EditTextPreference
        app:key="signature"
        app:title="@string/signature_title"
        app:useSimpleSummaryProvider="true" />
</PreferenceScreen>

Result (white text on buttons)

Empty new project. com.google.android.material:material:1.1.0
How to fix text color on EditTextPreference buttons?

A:

As it turned out in version 1.1.0 text color is taken from colorPrimary, not from colorAccent.
Details: https://github.com/material-components/material-components-android/issues/1009#issuecomment-585285178

Workaround: just use style Theme.MaterialComponents.DayNight.Bridge
Details: https://material.io/develop/android/docs/getting-started/#bridge-themes


---
title: Hover
component: Hover
description: Useful to render children conditionally based on hover state.
---

```js live removeFragment
<Hover>
  {isHovered => (
    <Box sx={{ height: 9, bg: isHovered ? 'red600' : 'blue600' }}></Box>
  )}
</Hover>
```

<PropsTable
  data={{
    children: {
      type: 'Function (isHovered: boolean) => ReactNode',
      description: 'A function to be called when on enter/leave',
    },
  }}
/>
26 experts on the security implications of emerging technologies have jointly authored a ground-breaking report – sounding the alarm about the potential malicious use of artificial intelligence (AI) by rogue states, criminals, and terrorists.

Forecasting rapid growth in cybercrime and the misuse of drones during the next decade – as well as an unprecedented rise in the use of ‘bots’ to manipulate everything from elections to the news agenda and social media – the report is a clarion call for governments and corporations worldwide to address the clear and present danger inherent in the myriad applications of AI.

However, the report – The Malicious Use of Artificial Intelligence: Forecasting, Prevention, and Mitigation – also recommends interventions to mitigate the threats posed by the malicious use of AI:

Policy-makers and technical researchers


New American Security, a US-based bipartisan national security think-tank; and other organizations.

The 100-page report identifies three security domains (digital, physical, and political security) as particularly relevant to the malicious use of AI. It suggests that AI will disrupt the trade-off between scale and efficiency and allow large-scale, finely-targeted and highly-efficient attacks.

The authors expect novel cyber-attacks such as automated hacking, speech synthesis used to impersonate targets, finely-targeted spam emails using information scraped from social media, or exploiting the vulnerabilities of AI systems themselves (e.g. through adversarial examples and data poisoning).

Likewise, the proliferation of drones and cyber-physical systems will allow attackers to deploy or repurpose such systems for harmful ends, such as crashing fleets of autonomous vehicles, turning commercial drones into face-targeting missiles or holding critical infrastructure to ransom. Thewe need to take ownership of the problems – because the risks are real. There are choices that we need to make now, and our report is a call-to-action for governments, institutions and individuals across the globe.

“For many decades hype outstripped fact in terms of AI and machine learning. No longer. This report looks at the practices that just don’t work anymore – and suggests broad approaches that might help: for example, how to design software and hardware to make it less hackable – and what type of laws and international regulations might work in tandem with this.”

Miles Brundage, Research Fellow at Oxford University’s Future of Humanity Institute, said:

“AI will alter the landscape of risk for citizens, organisations and states – whether it’s criminals training machines to hack


Stepping into their shoes: Reducing the actor-observer discrepancy in judgments of infidelity through the experimental manipulation of perspective-taking.
The goal of the current study was to examine whether perspective-taking could be an effective method for reducing the actor-observer bias seen in judgments of infidelity. Specifically, 708 adults judged the extent to which 32 behaviors were indicative of infidelity after being assigned to one of nine conditions in which the person engaging in infidelity (actor, partner, stranger) and the perspective-taking instructions (perspective-taking, stay objective, no instructions) were manipulated. Overall, the actor-observer and perspective-taking manipulations significantly affected judgments of the technology/online and solitary forms of infidelity. Adults in the perspective-taking condition judged their partner's and a stranger's technology/online behaviors as less indicative of infidelity than their own and their partner's solitaryQ:

Finding pronunciation correctness

I need to identify the "quality" of the user's pronunciation with the help of Microsoft speech SDK (System.Speech.Recognition). I am using MS Speech Engine - US, so what I actually need is to find out how close the speaker's voice is to the "North American" accent.
One way of doing this is by checking how close the user's voice is to the US English phonetic pronunciation. As mentioned in MSDN, it seems like this process is done inside the speech SDK by it self, so I need to get that out. Since we can set the phonetic to the engine by our selves as well, I am sure this is possible.
However, I have no clear idea about what I have to do. So, what can I


think these new vouchers are going to get things moving in a significant way. Anyone wanting to build wealth, in their mind, needs dollars

This is reflected in the reporting of other outlets, namely Bloomberg, who also notes that expensive BMWs are used as a currency hedge. This, of course, is only the tip of the iceberg when it comes to appropriately hedging your exposure to the peso for the ordinary Argentine citizen.

As a former financial planner, I checked things out over 5 years ago of what it would be like to help people to invest here. It turns out that any big money means you help them (illegally) invest outside the country. If you were to invest in a portfolio domestically, there is a requirement that HALF ofrun. As a matter of fact, I have never met anyone who saved pesos.

The conclusions that I'm forced to draw here is that investment in Argentina must be dollar based, and that if you can accomplish this, many Argentine citizens can probably provide you with some good business. From where I'm sitting, this is a pretty interesting investing dynamic, but the questions of "how" this is occurring, and "why" are worth exploring.

As a good American who's all too familiar with QE , the question of "how" such aggressive inflation could be occuring leads initially to the expansion of the currency supply. So, lets take a peek at their M1 and M2 levels.

Now, what is a central bank to do with all their fresh new money? Well, traditionally, you


Here's an interesting twist, after the judgment from the 2nd circuit came down, the risk of technical default from other bonds became more clear, as reported by Reuters,

If Griesa's ruling is upheld by an appeals court and Argentina still refuses to pay, U.S. courts could eventually block debt payments to creditors who took part in the debt restructurings out of consideration for investors who rejected Argentina's terms at the time. That would trigger a technical default on approximately $24 billion worth of debt issued in the 2005 and 2010 exchanges

If Argentina was less than keen to pay the holdouts the original $1.33 billion NML Capital was looking for, $24 billion would likely appear to be a material risk to Argentina's government. Their bond prices, as observed by J.P       CurrentTileMap().Tiles[BaseX + x, BaseY + y].floorHeight = 8;//heights[targetX+x,targetY+y];
                CurrentTileMap().Tiles[BaseX + targetX + x, BaseY + targetY + y].TileNeedsUpdate();
                Destroy(platformTile);
            }
        }
    }

    public void RaiseLowerBullfrog(int dir)
    {//TODO:Add a check for tiles at max/min height
        for (int x = -1; x <= 1; x++)
        {


(Artiodactyla) among mammals: Increased taxon sampling alters interpretations of key fossils and character evolution. PLOS ONE 4(9):e7062

Category:Prehistoric even-toed ungulates
Category:Prehistoric mammal familiesCastle, near Douglas, South Lanarkshire in the presence of the Duke of Hamilton, the Earl of Angus. Its recruiting area in Lanarkshire and Dumfries and Galloway was taken over by the King's Own Scottish Borderers and the Regimental Headquarters finally closed down in 1987.

Regimental museum
The Cameronians Museum is located within the Low Parks Museum, Hamilton, South Lanarkshire.

Traditions
Every new member of the regiment was issued a Bible, as a nod to Richard Cameron, after whom the original 26th Foot was named and the regiment mounted an armed guard at the doors of the Kirk during religious services. Soldiers wore a rifle green doublet with Douglas tartan trews as part of their full dress and No.1 dress uniforms. The regiment was one of only two in Britain to retain the


said. “Since we got her, she’s just been an awesome dog, so loving and such a good dog, so it’s almost like she knew that we took care of her, so she thought we could take care of her rescued kitten, too.

“Where Sadie’s never been a mom, I would never expect her to have those maternal instincts,” Thebarge said.

It started at about 2 p.m. Wednesday when Baldinelli, 17, looked out the window and saw Sadie carrying something. He said he thought it was a piece of trash.

“So I go outside and she drops a little kitten on my feet,” he said.

He said Sadie was carrying the kitten inside her mouth, with the kitten’s head hanging out on one side.

“She was carrying it like a little package,” Baldinelli said.Honeymoon Places in India

About Honeymoon in India

Honeymoon should be two things: exotic and romantic. Any newly wed couple would love to escape the crowd and celebrate intimacy in utter seclusion. India is one of the most sought after honeymoon destinations because when you are in India, you are never far away from sea, sand and snow.

From the whistling woods of Mussoorie to snowy landscape of Manali and romantic beaches of Goa to dramatic desert in Jaisalmer, India doesn't allow you to settle for anything less than an amazing experience. Be it the Paradise on Earth or God's Own Country, honeymooners can never be short of thrills. We guide you in planning your dream honeymoon with credible information on destinations and tour packages. Our articles and blogs help you


[NOT FOR PUBLICATION]

UNITED STATES COURT OF APPEALS
FOR THE FIRST CIRCUIT

No. 97-1951

UNITED STATES OF AMERICA,

Plaintiff, Appellee,

         v.

   SANDY PICHARDO,

Defendant, Appellant.

APPEAL FROM THE UNITED STATES DISTRICT COURT

FOR THE DISTRICT OF PUERTO RICO

[Hon. Hector M. Laffitte, U.S. District Judge]

       Before

Selya, Circuit Judge,

Coffin and Cyr, Senior Circuit Judges.

Mauricio Hernandez Arroyo for appellant.
Mark Irish, Assistant United States Attorney, with whom Guillermo Gil, United States Attorney, Jorge E. Vega-Pacheco, Assistant United States Attorney, and Nelson Prez-Sosa, Assistant United States Attorney, were on brief for appellee.

March 26, 1999
     Per curiam.  Following oral argument and careful consideration of the briefs and record on appeal, we reject as groundless appellant's claim that the district court erredcreate the vacuum in the container 22 of the adsorbent and one to activate the cooling 27. A structure gives rigidity to the vacuum-tight chambers 16 and 22, and a tube 26 connects the different elements together. This complex construction certainly does not make for a cost price compatible with a disposable package such as a can, and the reversibility of the intercommunication contributes to this complexity.
Other patents, the U.S. Pat. No. 4,759,191, supplemented by the U.S. Pat. No. 5,048,301 by the same inventors, illustrated in FIG. 3, propose the cooling of a beverage 15 contained in a package 10 by means of a module 11 placed in the package 10 (presented as a can).
This module 11 consists of several chambers. A first chamber 12 contains the liquid


these cooling means to pumping means external to the package. These pumping means cause and sustain the evaporation of a refrigerant liquid in the internal evaporator.
The internal cooling means and the external pumping means form the two distinct elements of the device according to the invention. They are connected by connection means but are independent in their design and manufacture.Gardai swooped on six members of an illegal money lending ring from the same family as part of an organised crime probe.

The arrests were carried out in the Ardmore Park and Ballybeg estates of Waterford city this morning.

Officers detained three men and three women from the same extended family who have bought up to 10 houses for cash despite being on social welfare.

A source said: “We believe they are illegal loan sharks and extorting money from people as well, along with being involved in the sale of drugs and then dealing in the sale and supply of tobacco.”

The arrests followed a search operation earlier this month which involved the Criminal Assets Bureau, regional armed support unit and local gardai.

Up to 30 officers were involved in yesterday’s activity which


by a 33 point margin.

Voters wish Barack Obama was still President instead of Trump 56/39. Trump is also losing badly to all the Democrats we tested against him in early 2020 match ups. He’s down 54-40 to Joe Biden, 53-40 to Bernie Sanders, 50-40 to Cory Booker, 51-42 to Elizabeth Warren, 47-40 to Kirsten Gillibrand, and 46-40 to Kamala Harris. The number for the various Democrats moves around some based on name recognition, but Trump stays consistently in that 40-42% range.

Trump’s approval rating this month is 41% to 56% of voters who disapprove of him. Much has been made of Trump’s numbers being so poor despite an improving economy, but only 32% of voters actually say their economic situation has improved in the last year. 22% think it’schronic disease often characterized by relapse which, if unaddressed, may lead to continuing and increasing criminal behavior and other personal, family, and societal problems.

The Drug Court is an opportunity to direct those in crisis with addictions to begin a rehabilitation process which might ultimately lead to a reduction or elimination of their addiction and permit the development of a productive lifestyle.

There must be immediate intervention, which permits the treatment option to be made available to a defendant at the earliest possible time while this person is still in crisis.

Defendants will be evaluated by trained staff as early as possible so they can be given an opportunity to take advantage of the resources offered through a Drug Court.

Defendants must be accountable for their behavior and must take responsibility for


ensuring their own recovery through the options made available to them by the Court and the community. Defendants accountability will be enforced through participation in the program as outlined in the program plan and written agreements. Individual service plans will be developed to address their addiction and other related problems. There will be immediate recognition for successes as well as immediate and
appropriate consequences for failure to perform.

An evaluation that measures the outcomes for individual defendants in the process of the Drug Court program is essential. That part of this evaluation is an annual report distributed to all members of the Drug Court team setting forth the incidence of recidivism among drug court graduates as derived from available justice system resources.

That participants entry into the Drug Court Program shallwere feasible in all procedures. Mean radiation dose of the procedure was 60.21 Gycm2 (range 55.02-63.75 Gycm2). The mean total procedure time was 32.2 min (range 27-38 min). The mean fluoroscopy time was 12 min and 3 s. The mean procedural iodinated contrast material dose was 24 mL (range 20-40 mL). IF gives Interventional Radiologists the opportunity to use new technologies in order to improve outcomes with a significant reduction of contrast media administration.


in June or July of 1976, Morgan shot at Roberts and attempted to run him off the road because of Robert's relationship with Taxpayer.  Morgan also threatened to kill the Taxpayer and her son from a former marriage.  These events evidently prompted her to file a divorce petition in June of 1976 in order to terminate her marriage.  Taxpayer supplied her divorce attorney with the records and files Morgan kept on various real estate transactions.  Taxpayer later retrieved these documents and delivered them to Ragland for his review which led to the discovery of Morgan's receipt of the kickback.

15
Significantly, Taxpayer expressly alleged in her divorce petition that Morgan might have incurred federal income tax liability, that he had concealed community property, and thus shouldcash.  She disputes the Tax Court's characterization of her life style as "comfortable and expensive," even though she testified that she and Morgan occupied a recently-purchased and fully-furnished home adjacent to a private country club golf course, drove relatively new automobiles and flew to Colorado for a winter vacation.  The life style she enjoyed during 1975 coupled with her own recognition that she had a personal gross income of only $3,819.00 and that she and Morgan were living beyond their means make it reasonable to conclude that she at least had reason to know that Morgan was supplementing the community coffers with income he had earned from transactions, including Singing Hills, which Taxpayer knew he had participated in during 1975.

39
Taxpayer also testified that Ragland's demands for


were subsequent to the required filing date (April 15, 1976).

59
Taxpayer argues strenuously on appeal that the Tax Court erred in its determination of the reasonable cause issue and claims that such determination, in light of the testimony presented at trial, is unjustifiable.  In reviewing this finding by the Tax Court, we are guided by the Supreme Court's declaration that whether the elements that constitute "reasonable cause" are present in a given situation is a question of fact, but what elements must be present to constitute "reasonable cause" is a question of law.  United States v. Boyle, 469 U.S. 241, 250 n. 8, 105 S.Ct. 687, 692 n. 8, 83 L.Ed.2d 622 (1985).  It is well-settled that in order to avoid the penalty prescribed by section6651(a), Taxpayer bears the heavy burden of proving that the failure did not result from "willful neglect," and that the failure was "due to reasonable cause."    26 U.S.C. Sec. 6651(a)(1).  Boyle, 469 U.S. at 246, 105 S.Ct. 690.    In this case, willful neglect is not at issue.  The central focus of our inquiry therefore is whether the Tax Court properly determined that no reasonable cause existed to excuse Taxpayer's delinquent return filing.  In accordance with Boyle, we shall review the Tax Court's findings regarding the existence or presence of circumstantial factors which might give rise to reasonable cause under the clearly erroneous standard.  See also Millette & Associates, Inc. v. Commissioner, 594 F.2d 121, 125 (5th Cir.1972).

60
A review


palatable foods [@pone.0085679-Pelchat1]. A later study [@pone.0085679-Pelchat2] repeated this design with a shorter monotony phase (1.5 days) and involved functional magnetic resonance imaging (fMRI) while participants imagined their favorite foods. Participants after a monotonous diet activated hippocampus, insula, and caudate more than participants on a normal diet, indicating that hedonic hunger activates brain regions implicated in sensory integration and memory. Other evidence for hedonic hunger effects is rather indirect: animal research has demonstrated that rats will work hard and endure adverse conditions to gain access to palatable food, even if standard lab chow is available [@pone.0085679-Oswald1] and that gastric filling in the absence of oral sensoric stimulation does not terminate eating [@pone.0085679-Kissileff1] indicating the dominance of hedonics over homeostasis in some cases. In humans, short term experimentally inducedpresented for 6 s and were separated by a 10--12 s ITI. During 70% of the picture trials, startle probes were presented, either at 3.5 s (43%) or 5.5 s (57%) after picture onset. Furthermore, startle probes were presented during 20% of the ITIs (8 startles, at 6 s). After each block, participants used visual analogue scales to rate whether they felt "frustrated" or "depressed" ("0- not frustrated/depressed at all" to "10- very frustrated/depressed") during the previous block. During a subsequent rating phase, each picture was shown again and rated on visual analogue scales on palatability ("0- not palatable at all" to "10- very palatable,") and desire to eat ("0- don't like to eat this now" - "10- would like to eat this now").

Taste Test {#s2f}
----------

After the task


attentional processes (measured, e.g. though the P3 amplitude to image and startle probe [@pone.0085679-Bradley1]) to startle modulation. Fourth, since the present project tested several related questions, participants had completed a go-nogo task prior to the present task. It is possible that this involved certain priming effects or reduced concentration. However, these would affect both sessions to a similar degree. Last, conceptually, this research was inspired by the observation that eating disordered patients hedonically deprive themselves through 'blacklisting' chocolate and other high-energy foods with the result that eventual consumption of these foods often trigger binge eating (loss of control). Successful cognitive behavioral treatment involves a reintegration of such foods into the diet to reduce such deprivation effects. Thus eyeblink startle might be useful as an outcome measure of such

**Image selection.**

(DOCX)

######

Click here for additional data file.

The article processing charge was funded by the open access publication fund of the Albert Ludwigs University Freiburg.

[^1]: **Competing Interests:**The authors have declared that no competing interests exist.

[^2]: Conceived and designed the experiments: JB EN. Performed the experiments: EN. Analyzed the data: JB EN JS. Contributed reagents/materials/analysis tools: JB. Wrote the paper: JB EN JS BMH BT.


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Last year we wrote about News Corp. CEO Robert Thomson's weird anti-Google rant that seemed quite devoid of facts. It was a odd amalgamation of conspiracy theory and outright falsehoods that fit more in the realm of an angry internet troll, rather than the CEO of a media conglomerate. Apparently, Thomson has continued to let these beliefs fester and has once again gone off on Google and other companies much more successful on the internet in a confused and rambling speech in Australia . The talk, focused on the future of the media industry, starts off with a philosophical discussion about China before jumping into an attack on the internet, which begins mildly enoughwith some digs at internet companies:Of course, this gets pretty much everything wrong. They're not, they tend to be. And there are all sorts of things that are just silly about this. After all, News Corp. itself has a rather long history of building or buying its own aggregation tools that tend to be a lot more aggressive than the Googles or Facebooks of the world. Oh, but nearly all of News Corp.'s attempts have flopped The reality, it seems, is that Thomson is jealous that Google and Facebook have succeeded in building, whereas News Corp. has failed. So he lashes out claiming that they're "pirating" or "stealing" content, and "redistributing" it. But that's not what's happening at all. Google is a, it's helping people find more content.


Q:

Different Image processing results from Java and Matlab

I was doing a simple image processing task which I prototyped on Matlab, then made a port to Java. After executing the task on Java, I noticed different results. Is this a platform specific issue?
The task I was doing so far involves obtaining an image's green channel, computing the percentage of a number of pixels with values of a certain range, and computing the arithmetic mean of that channel. Code and results are shown:
Matlab
   a = imread('large.jpg');
   g = a(:,:,2);
   [x,y] = size(b);
   count = 0;
for i=1:x
    for j=1:y
        if g(i,j) < 90 & g(i,j) > 30
           count = count + 1;
            %Mistake part for arithmetic mean. Thanks to james_alvarez
            g(i,j) = 255;
        end
    end
end
 count
 (count/(3000*2400))*100
 mean2(g)

----------

1698469
23.5898
165.9823

Java
BufferedImage img = ImageIO.read(Function.class.getResource("large.jpg"));
int gCount = 0;
int meanCount = 0;

int w = img.getWidth();

int h = img.getHeight();
for(int i = 0 ; i < w ; i++){

        for(int j = 0 ; j< h ; j++){

            Color c = new Color(img.getRGB(i,j));
            int g =


(CNN) The Food and Drug Administration dealt another blow Tuesday to supporters of kratom, warning about "deadly risks" associated with the botanical supplement often used to treat pain, anxiety and depression. Kratom also has opioid-like effects -- at low doses, it acts like a stimulant; at higher doses, it sedates, dulling pain -- which has also made it popular as an over-the-counter remedy for opioid withdrawal.

"It's very troubling to the FDA that patients believe they can use kratom to treat opioid withdrawal symptoms," FDA Commissioner Scott Gottlieb said in a statement . "There is no reliable evidence to support the use of kratom as a treatment for opioid use disorder."

Instead, he said, the FDA is concerned that the use of kratom could actually "expand the opioid epidemic."

"We've learnedSubsidizing the Corporate One Percent: Subsidy Tracker 2.0 Reveals Big-Business Dominance of State and Local Development Incentives

by Philip Mattera

February 2014

As a result of substantial enhancements we have made to our Subsidy Tracker database, it is possible for the first time to estimate the share of total state and local economic development awards going to the largest corporations.This report summarizes the findings.

full report

press release

Subsidy Tracker 2.0 database

Top 100 Parents

Top States


nonprofit devoted to improving the way Omaha looks, functions and feels.

The Omaha Community Foundation (OCF)

One of the country’s largest community foundations, OCF connects people who care about the community with the people and nonprofits who are doing the most good here. In addition to supporting Omaha’s philanthropic community, OCF works to determine the Omaha community’s greatest needs; to build up its nonprofits; and to find new ways for people to come together to give. The OCF will occupy one office in the CEC to improve their proximity and outreach among local nonprofits and UNO students, faculty and staff.

Omaha Public Library

OPL strengthens our communities by connecting people with ideas, information, and innovative services, a mission that will be better achieved by working collaboratively with other organizations. In 2012 OPLat work and at home.

William Brennan Institute for Labor Studies

A UNO institute dedicated to fostering creative and critical thinking among labor leaders, potential leaders, and interested members by providing relevant information and training in the skills needed in today’s changing economy and workplace.

WhyArts? Inc.

A community organization that partners with professional artists and area organizations serving at-risk youth, people with physical, mental or developmental disabilities and seniors to create interactive arts experiences.


gives birth, Silver says it is a relief to know that the pregnant mares will be able to deliver their foals in peace.

“I feel really proud that we were able to save them. But now we’re responsible for their lives, and we’re going to have babies on our hands, all who need to be trained and cared for,” she says. “The excitement and the stress of the auction is over. But it’s only the beginning. To me, what happened to these broodmares is absolutely shocking. I know they send horses to slaughter. But to send pregnant mares who have full-term babies in their bellies, is shocking. Nobody else bid on these mares except kill buyers.”

But, there were many kind-hearted souls who stepped up to help the mares whenhung from one. Have a great day. And please make no further comments to me because I have no intention of answering you or paying any attention to your rudeness

Wow guess you have some serious anger issues Lynn. Doesn’t matter what anyone says on here, you just take offense. How you have gotten this thread so off course is unreal. Maybe its time ti just go back to something simple….like how are the rescue mares doing?

Many dog breeders are part of the problem. The other very large part being irresponsible owners. But there are in fact responsible breeders (many of whom also participate in rescue for their breed) who insure via contract that any dog they breed is returned to them at any point in the dog’s life


date, they haven’t saved a single horse even when asked to help truly needy horses. All their supporters claim to love horses, but they throw their money away going after horses that already have a good home.

I’m really glad this rescue was able to save these mares. I agree that late term mares (and mares with foals at their side) should be exempt from being sent to slaughter. I also have no quarrel with responsible breeders like Lynn.

why are horses different then cows or pigs or chickens or turkeys ? ANIMALS ARE OUR FRIENDS – THEY ARE NOT FOOD because we have a SELFISH, SELF CENTERED 5 MINUTE URGE to eat their flesh , what a selfish way humans think , some are “food” and some are not,who says so ? the greatest thing a human being ( with such “big brains” ) certainly can figure out that TO DO NO HARM TO OTHERS , is the moral thing to do PERIOD . I feel sorry for all the animal murderers out there who can’t figure this out and who go around with the CANCER CAUSING HORMONES AROUND INSIDE OF THEIR BODIES & INTESTINES FOR MONTHS & YEArs at a time it takes to digest the flesh of OTHERS , they carry around DEATH, MISERY, DISMEMBERMENT , SUFFERING & , HORROR inside of them all for their “appetite” for OTHERS flesh . if there where more people like this man in the world we would SURELY have a kinder, better more peaceful place to live


He was sold to a good home. I’ve also taken back horses I’ve sold when the new owner couldn’t keep them.
I have never sold a horse at auction. In fact, I once bought a weanling to keep him away from an auction.
I don’t like slaughter. I understand how awful it is. But, I’m also a realist. Until the do-gooders on this page can find a practical alternative, you all need to stop slamming other people who deal with reality.

I deal with the reality every day. I have 5 TBs here 4 are OTTBs, all rescues. I run a horse rescue in total we have 7 TBs, and 22 horses in total in the rescue. So I live the reality every single day. I also work every single dayto deal with is cowardly and wrong.
I’m still waiting to hear a realistic means of dealing with unwanted horses. Rescues are great, but there just aren’t enough.

You keep forgetting that nothing trumps food safety. It doesn’t matter how many unwanted horses there are…. however, to put into perspective…. the number of horses going to slaughter accounts for less than 1% of the total horse population in the US. Those numbers will be affected by natural attrition, people will have to euthanise instead of trying to get the last 50.00 out of their horse, they will have to be more responsible with breeding. Breed registries will have to step up to the plate and help provide after care. Those horses going to slaughter over a years time can be


absorbed easily.

Horse slaughter though, as an aside, can never be made humane…. horses anatomy and physiology means there is no head restraint which works, and in Canada, horse slaughter is actually better regulated than it ever was in the US…. it still is a huge problem. We still have a problem with neglect etc… because slaughter does not prevent neglect,,,, Slaughter does encourage excess and bad breeding…

But again…. bottom line is, we do not raise horses in this country for food…..

Your solution might work in a perfect world, but the world isn’t perfect. One of the biggest problems with euthanasia is the high cost. I had a boarded horse whose owner retired and was no longer able to afford her. He agreed that if we couldn’t find athese animals. No compassion should be given to them. They are the worst of the worst and then some. There should be limitations on the breeding of TBs and all horses, so all have a chance for a good, loving, compassionate home with hugs and kisses coming their way. I wish I could save them all because they would plenty of hugs and kisses. Mine get them.

I am 70 and riding a horse again is near the top of my bucket list. I would care for one of these mares just to save her life. I miss the smell of a horse and sweet feed. There is nothing like sitting atop a horse on a beautiful day being totally in the moment.

Margaret. Congratulations on your being on a


Boston College, even though our Swan is missing one of Proust’s Swann’s “n’s.” Leslie’s father and uncle, Philip G. and Julius J. Epstein, wrote “Casablanca,” among other movies. His son, Theo, is the General Manager of the Boston Red Sox. His daughter, Anya, was the head writer and a producer of “Homicide: Life on the Street” and his son-in-law, Dan Futterman, was nominated for an Oscar for “Capote.” We’re trying to get Leslie to retire to Old Friends. You can’t fault Leslie or Swannie’s bloodlines.

Please be polite we still dont have paperwork for the foals again please don’t go crazy we are awaiting the stallion reports there will be time to do it. I am concerned that this article could cause us to not get them and ifone. God Bless you for all you do. It is also happening to our “so-called” Federally Protected Wild Horses. Thousands that were sold to a known kill buyer, Tom Davis, for $10.00/ head cannot be accounted for~~on the papers he had written they were being sent to Mexico to be “in the movies” Unfortunately, it can be a very good income for someone with a heart of stone. Buy for $10/ sell for $100.00 right across the border. This one individual has bought @ least 1700 Mustangs and the BLM woman who worked with him was given a promotion for adopting so many Mustangs out to new homes. The whole idea of slaughter makes me sick.

Not saying this is the responsible party, but R.N. Miller of PA is


one name that pops up in the history of these mares & their previous foals. Questions, of how they ended up at New Holland, might start with him/her.

Peter Giangiulio (PA) came up on two (doing breeder searches on the mare’s progeny. I don’t expect my post to be approved, given these names being mentioned, but am hoping it will be passed along to the authorities needing to know. Thank you & keep us posted please.

I am so happy to hear all of these horses are safe. However, I would never ” thank” anyone in the slaughter chain for “help” when they drove a horse “back” [to safety]. Why is it so easy to forget that the son did not drive that horse to safety as an act ofin the wrong business for sure

I will say I would have trouble believing that any of these mares came from a TB breeding facility if there isn’t explicit proof. I can’t imagine a farm spending thousands of dollars for 9+ months of prenatal care for four horses and then dumping them.

I hope they all foal out safely, and that they and their babies will be healthy going forward, but I do hope that the TB world isn’t vilified because of where they were found. Thank goodness for the people who saved them.

the thoroughbred world needs no help in “villifying” them they do a GREAT job at this all by themselves , you are talking about people who BREED & USE animals SPECIFICALLY FOR PROFIT , they cull the


in prizemoney alone. She herself was well bred, had won in racing and was bred only with top sires and was 19 years of age when she went through the sales, went for a song and not surprisingly I came to a dead end as to her outcome. Some horses are put through purely for tax benefits so for her service to the industry she was disposed of and highly likely went to slaughter.
I was born into a racing family which has been racing horses since the early 1800s and to this very day some extended family continue to carry on with the family tradition. About 20 years ago my father was informed that some of the family’s horses were being mistreated. Rather than sit in the Members’hand on these horses before they ended up at New Holland, no one will work with the rescue. Bottom line is…the rescue is still working on this information. The horses are safe and being well cared for. If anyone really wants to help, help the horses by donating to Gerda’s Animal Rescue through paypal at gerdasilver1@hotmail.com

Thank You, Gail, for the voice of reason!!! All you armchair specialists, just simmer down, let them handle it the way they know best. The horses are now in safe hands, lets all take a deep breath. And, Thank You, Rescue people!!! You are so very needed!


of at least one polymeric material, at least one monomer, at least one polymeric precursor, and mixtures thereof to deposit the component on the substrate and form a coating thereon, then imagewise exposing the coating to radiation such that exposed and unexposed coating portions are formed, and then subjecting the coating to a second composition comprising carbon dioxide such that either one of the exposed or the unexposed coating portions are removed from the substrate and the other coating portion remains on the coating to form an image thereon (e.g., developing the image using carbon dioxide). For the purposes of the invention, the first and second compositions may be the same or different. In a preferred embodiment, each step may be performed in a closed system such thatThese duller, paler and smaller particles also remain in liquid suspension longer than the more intense, darker and heavier particles, which sink first to the paper surface and into the paper crevices. In very thick applications, the lighter particles form a thin sediment on top, adding a "white" component of scattered light and obscuring the brighter color underneath.

You control these paint attributes through your choice of watercolor brand, and you can guide your choice by carefully testing your watercolors for tinting strength and particle size. I find a simple yet revealing test is to mix a moderately diluted solution (roughly 1 part paint to 6 parts water) of the paint, pour the solution into a shallow mixing pan or palette, and let the solution completely evaporate. Variations in


(R), because this breaks up the paint surface and provides greater texture for the pigment to lay on. But this rougher surface makes the multiple glazes harder to do, because the edges of color areas are randomly broken up by the paper texture, and these edges are harder to match with each new coat of paint. On the other hand, hot pressed (HP) papers are in general the least absorbent, and pigments are more likely to pool and dry slowly on a hot pressed surface. If you need to use a juicy wash, you will have to wrestle with blotching and backruns caused by the puddling water. The is the reason for using a smaller brush, which applies smaller quantities of paint.

Finally, the glazing method is really laboror consistency.

 Creamy concentration (1:3). Add 2 more teaspoons of pure water, each time using the 1/2" acrylic brush to clean the spoon further. Switch to the 1/2" sable flat brush, and stir the mixture until the paint mixture is completely smooth, checking for any undissolved lumps of paint lurking in puddle. The mixture should now have the viscous consistency of cream or olive oil. If daubed on a bare area of palette, the mark will slowly and partially contract its shape, but will not make a round puddle; if you drag your brush through the mixture, you will see the surface underneath for a few seconds before the paint closes over the trace; if you wick the brush against the side of a paint well, the paint


tonal value is dark, and for small areas of dark values, or for drybrush textures (prepared by flicking a damp brush across a pile of raw pigment), this concentration is perfectly usable.

Paint at a syrupy concentration dries to essentially the same pigment concentration as raw paint, with approximately the same lightness (value). It is not much easier to apply with a brush. The paint flows thickly and reluctantly, and is awkward to spread in an even layer. The main problem is that paint at this concentration will almost always bronze (acquire a sticky, leathery or shiny surface texture) in areas where the paint puddles slightly  as it has done along the left side of the illustration swatch. In fact, bronzing is usually more obvious and uncontrollable atcolors  tend to separate more quickly and more visibly in these weak washes, producing beautiful mottled variations of contrasting color. (In the examples above, you can see separation of the brown burnt sienna and blue ultramarine in the two lefthand bottom samples.) Use each dilution to best effect: bright and flat color from creamy concentrations, subdued but textured color in watery concentrations.

Finally, notice how  just by adding more water  the color quality in these four dilute swatches changes from the colors of a stormy sky to the pearly grays of dawn. Always explore the full range of a paint's color qualities, not just the color in heavy mixtures.

These paint:water ratios are guidelines only. The dilution ratios and liquid texture depend on each brand's paint formulation.


all saturated yellows start at a lightness above 80. Then the dilution curves flatten out, and the proportional increases in dilution necessarily get larger with each step. The saving recourse here is that, if you have established regular value steps in the darker values below 80, these lighter value changes are pretty easy to judge by eye.

Mixing Recipes for Lightness Steps. The relationship between dilution and lightness can be analysed systematically, although this may require you to go through a lot of trial and error. I have found, too, that this method is useful for photorealist paintings that require exquisitely exact modeling (for example, figure nudes), but is otherwise much to cumbersome for the practice of wholesome art. Careful measurement should not be your standard working procedure, butoatmeal-like water substance, so I was OK with that.

Normally around 5:30 when I finish work I’m VERY hungry. Today I wasn’t, which was likely due to the late soylent lunch. I went for a swim (1.5km), which also normally leaves me pretty hungry and indeed I was. At about 8:00 I had a late dinner at Boston Pizza with some friends.

Day 2

Stomach was definitely upset this morning, but not sure if that was from the soylent or the Boston Pizza. I had the regular breakfast and headed out to school.

This time I ate soylent at a more lunch-appropriate time (12:15), with no snacks between breakfast and lunch. Again, having some trouble finishing the entire 24oz. I stopped at the same amount as yesterday. I was actually fairly hungry


lunch. That way I don’t need to keep awkwardly sized tupperware containers around. I think there are plenty of other places I could optimize this process, but I’m still in the “having fun making this” phase so I’m not too worried about it. Setup, measuring, mixing, cleaning, etc all together took around an hour I believe. Not bad for three days lunch. This time I tried adding some vanilla extract (4 cap fulls) in order to account for the fact that I used unflavoured vanilla whey protein. It wasn’t immediately noticeable in the taste, but maybe it’ll be stronger in the morning.

Day 6

Back on soylent!

The vanilla taste isn’t as strong as I hoped, but that’s OK. The “oatmeal” taste is far less powerful. I ate around noon anddomains *in vivo* is unclear: some studies reported that MeCP2-binding tracks the density of mCpG[@b4], but another study found that the majority of promoters with the highest methylation levels are not bound by MeCP2 (ref. [@b8]). To investigate whether DNA methylation regulates MeCP2 binding in olfactory epithelial tissue, we mapped methylated cytosines using whole-genome bisulfite sequencing in two biological replicates. The CpG methylation levels, defined at each CpG site to be the fraction of cells having methylation at the site, agreed well between the samples (*r*=0.89 in 10 kb windows, [Supplementary Fig. 1k](#S1){ref-type="supplementary-material"}), and the replicates were combined for downstream analysis. The methylation level also had strong overlap with previous studies in frontal cortex[@b24] and embryonic stem cells (ESC)[@b25] (*r*=0.79 and *r*=0.76, respectively, [Supplementary Fig. 1l,m](#S1){ref-type="supplementary-material"}). The genome-wide CpG


elevated ([Fig. 3a](#f3){ref-type="fig"}). Contrasting this, distal CGIs were enriched for mCpG%, GC% and MeCP2 binding ([Fig. 3a](#f3){ref-type="fig"}). Thus, while MeCP2-binding sites are enriched for both high GC% and mCpG%, the binding profile generally follows GC%, even where GC% and mCpG% diverge.

A recent study argued that MeCP2 also recognizes methylated cytosine in the mCpH context (H=A,T,C)[@b27]. To systematically investigate the importance of mCpH%, we next built a Gaussian graphical model for MeCP2 binding and explanatory covariates. Briefly, a Gaussian graphical model represents the conditional dependence of random variables as an undirected graph, where the nodes are the variables of interest and an edge between two nodes captures the correlation between the connected nodes after controlling for confounding correlations with the remaining nodes. We first built a model relating the3c,d](#f3){ref-type="fig"}). The hypothalamus data set showed a stronger edge between MeCP2 ChIP and mCpH% than between MeCP2 and GC% ([Fig. 3e](#f3){ref-type="fig"}), but that data set had a very strong correlation between MeCP2 and Input, making it difficult to interpret the result. Because one data set had very low counts at 150 bp resolution ([Fig. 3f](#f3){ref-type="fig"}), we repeated the analysis at 10 kb resolution and found graphs with similar structures, the main difference being strong correlations between MeCP2 and Input in ESC, hypothalamus and forebrain ([Supplementary Fig. 6f--j](#S1){ref-type="supplementary-material"}). Our integrative analysis thus shows that GC% and, to a lesser extent, mCpG% are the main predictors of MeCP2 binding.

MeCP2 preferentially binds nucleosomal DNA
------------------------------------------

Given the role of MeCP2 as an epigenetic regulator, we next investigated the interplay between MeCP2 and chromatin structure. Previous


into separate WT and KO groups ([Supplementary Fig. 9a](#S1){ref-type="supplementary-material"}). An earlier study found that the majority of MeCP2-bound promoters are actually transcriptionally active, suggesting that even if MeCP2 acts as a repressor, its binding may not completely silence gene expression[@b8]. To investigate the relation between MeCP2 binding and gene expression, we sorted genes by their expression levels in WT animals and aligned the MeCP2 ChIP enrichment around the TSSs ([Supplementary Fig. 9b](#S1){ref-type="supplementary-material"}). While MeCP2 is strongly enriched downstream of the TSS, this enrichment did not correlate with expression status and was almost constant for FPKM values between 0.1 and 50 ([Supplementary Fig. 9c,d](#S1){ref-type="supplementary-material"}). In the region upstream of the TSS, the MeCP2 ChIP enrichment was marginally lower in highly expressed genes compared with lowly expressed genes, particularly inunderlining stromal cells (8.8%) (data from this study). As a result, distinct individual MeCP2-binding sites could be identified, and only very low correlation was observed between MeCP2 ChIP-seq and Input data, distinguishing our results from previous published data sets[@b9][@b27]. We believe the preponderance of a unique neuronal population in conjunction with an unconventional deep-sequencing strategy made our high-resolution data possible.

While our data exhibited the previously reported enrichment of CpG in MeCP2 peaks, we found that the GC% is the predominant predictive feature and that the correlation between MeCP2 binding and CpG% is confounded by the correlation between GC% and CpG%. Integrative analysis further showed that GC% is the dominant feature associated with MeCP2 binding even after including methylation information. This observation was further supported by reanalysis of published


dounce homogenizer, nuclei were collected by centrifugation. Collected nuclei were washed once, resuspended in the MNase buffer and digested with 0.5 units of MNase (New England Biolabs, Ipswich, MA, USA) per microlitre volume for 15 min at 37 °C. MNase digestion was stopped by putting the samples on ice and adding EDTA to a concentration of 10 mM. After digestion with 0.1 μg μl^−1^ RNase A (Fermentas, Pittsburgh, PA, USA), DNAs were purified with DNA Purification Magnetic Beads (Life Technologies, Grand Island, NY, USA), and pellets were dissolved in H~2~O. DNA fragments corresponding to mononucleosomes (about 150 bp) were confirmed by using 2100 Bioanalyzer (Agilent Technologies Santa Clara, CA, USA). MNase-seq libraries were prepared as described (Bioo Scientific, Austin, TX, USA) using 5140-01 NEXTflex DNA Sequencing kit and 514101 NEXTflex DNA Barcodes-6. High throughputsites, intron/exon boundaries, CpG-islands---all downloaded from the UCSC table browser[@b48]---and the MeCP2 enrichment-peak centres. The signal tracks were the ChIP-seq and Input coverage, the MNase-seq nucleosome occupancy, GC% and CpG% in tiled 50 bp windows, and the ENCDOE 50 bp mappability track. The former three were normalized by their respective genome-wide mean. Plots of MeCP2 enrichment display the ratio of the pixelated MeCP2 ChIP and Input maps, normalized using signal extraction scaling. For a more quantitative display of the data, we also grouped the alignment points into quartiles of the ordering score and plotted the median signal profile for each quartile.

GC and CpG dependence of ChIP- and MNase-seq signals
----------------------------------------------------

To clarify how the MeCP2 ChIP enrichment and MNase-seq coverage depends on GC% and CpG%, we first tiled the genome with 150 bp


between sequence and signal, we also displayed the contours of the empirical measure *p*(*f*~GC~, *f*~CpG~), each contour labelled by the enclosed genomic fraction. Because CpG is much rarer than CpC, GpC and GpG, the region was under sampled and we masked it in the plot.

Bivariate distributions
-----------------------

We created the bivariate distribution plots of the ChIP-seq coverage and nucleosome occupancies ([Supplementary Fig. 1a](#S1){ref-type="supplementary-material"}) by first tabulating the number of times the two signals *s*~1~ and *s*~2~ are observed as a pair (*s*~1~, *s*~2~) and then normalizing these counts to get an empirical density function. The contours of this density function were then created by first interpolating between the observed points (*s*~1~, *s*~2~) and then placing contours evenly between zero and twice the mean density in the plotted region. To create[Fig. 1b](#f1){ref-type="fig"}, and the AUC of ROC is 94%. We investigated robustness of our results with respect to varying depth of regression trees and found that AUC varies \<1%, and correlation between prediction of MeCP2 enrichment and its observed values varies \<2% as the depth of regression trees varies between 3 and 8.

Next, we added the dinucleotide concentrations into the feature set. Since we now have multiple features in the set, we are interested in how important the individual features are for the prediction of MeCP2 enrichment. The average relative tree-depth of a feature used in trees of the forest is used to quantify the relative importance of the features on the predicted value. Our Random Forest regressor showed that dinucleotides have very small importance and hence a


di-nucleotide GpC has importance of 2.3% and the highest trinucleotide CAG has importance of 0.5%. Similar results were obtained as depths of regressors ranged between 3 and 8.

Expression analysis
-------------------

The RNA-seq reads were aligned to the mm9 refGene using TopHat 2 (refs [@b49], [@b50]). Only reads mapping uniquely, in proper pairs, and to the same chromosome were retained, leaving 65, 55 and 56 million reads in the WT replicates and 58, 51 and 51 million reads in the KO replicates. Differential gene expression and statistical significance were calculated for the refGene transcripts using cufflinks and cuffdiff[@b51], ran with default parameters but keeping only uniquely mapped and proper read pairs.

Gaussian graphical models
-------------------------

In a Gaussian graphical model the weight of the edge between two random variables *X*~*i*~ and *X*~*j*~ is the(**f**) Same as **b** but using MeCP2 ChIP and Input from Gabel *et al*.[@b9] and methylation data from Lister *et al*.[@b24]](ncomms11025-f3){#f3}

![MeCP2 binding coincides with nucleosomes.\
(**a**) Enrichment plot of WT MNase-seq profile (colour) around MeCP2 ChIP-seq peaks (sorted by length and outlined by dashed curves). (**b**) GC% and CpG% dependence of the mean WT MNase-seq coverage (colours). The contours indicate the genome-wide joint distribution *p*(GC%, CpG%). (**c**) MeCP2 enrichment as a function of GC% and MNase-seq fragment coverage. The contours indicate the genome-wide joint distribution *p*(GC%,MNase-seq).](ncomms11025-f4){#f4}

![MeCP2 binding at the 5′ end of genes is associated with increased transcription in *Mecp2* KO.\
(**a**) Top plot shows the alignment of MeCP2 enrichment profiles (colour) in 8 kb regions surrounding the significantly up- and down-regulated genes after *Mecp2* KO (separated by thick horizontal black


expression of olfactory marker protein (OMP, green) and the nuclei were identified by DAPI staining (blue). No significant changes in cell body and nuclei sizes were observed between wild-type (identified by MeCP2 expression (Red), left panel) and KO (shown by lack of MeCP2 expression, right panel). Scale bar, 20 μm. Quantification is shown in [Supplementary Fig. 10](#S1){ref-type="supplementary-material"}.](ncomms11025-f5){#f5}

[^1]: These authors contributed equally to this work.

[^2]: These authors jointly supervised this work.
prioritized an early increase in carbon sequestration for species and countries with the lowest opportunity costs. The last three columns of Fig. [3](#Fig3){ref-type="fig"} show the fraction of the maximum sequestration potential of the corresponding country and species to be exploited for the optimal implementation of CSF. We observed an early allocation of CSF to *Picea abies* in eastern European countries and *Fagus sylvatica* in Spain, Slovakia and Poland. Essentially, optimized CSF prioritized sequestration in these low-cost areas, whereas central Europe and Scandinavia remained as areas focusing on wood production. With increasing preference for carbon sequestration, low-cost areas were not capable of providing enough sequestration potential, demanding an increase in sequestration in Scandinavian countries and Germany (areas with high sequestration potential), while balancing carbon sequestration and forest profitability. Aiming for


arising from climate change mitigation and *in situ* carbon sequestration strategies, indicating that efforts to sequester carbon in one region might result in increasing emissions elsewhere (26). However, as Gan and McCarl^[@CR24]^ suggested, leakage effects might be hindered through cooperation between countries. Moreover, analyzing the life cycle of wood products to quantify their substitution effects for energy-intensive materials and fossil fuels may highlight the further positive effects of forest sector for carbon mitigation^[@CR20]^. Ideally, this point should be considered in the studies of forest adaptation and mitigation under climate change, providing a holistic analysis of mitigation options. Additionally, further research may include the ecological response of forest ecosystems to changes in environmental conditions^[@CR22]^ and the short and long term adaptive measures necessary to establish resilient ecosystems in theproduction system (supply side) to internally define the changes in price and costs endogenously.

CSF as applied in this study may achieve, for example, 48% of the total sequestration potential with 78% lower total costs compared to the maximum carbon sequestration. However, the carbon cost in Europe might impose a great obstacle to significantly increase the sequestration *in situ*, as the prices of the Emissions Trading System (EU ETS) decreased from 30.00 EUR/ton in 2008 to 7.70 EUR/ton of CO~2~ in 2015 (110 and 28.23 EUR/ton of carbon respectively). At current price levels, the additional carbon sequestration considering all countries and species included in our study would increase on average from 4.7 Billion PTE (58.8 Million PTE/year) to 7.9 Billion PTE (98.4 Million PTE/year), corresponding to 7.7% of European


regimes while simultaneously considering NPV maximization and carbon sequestration. We established a trade-off curve of NPV and carbon sequestration, i.e. the Pareto frontier of NPV and carbon sequestration. The weights of both objectives were varying for each species, country, and climate change trajectory through the global optimum solutions of the WGP model. The deviations from maximum NPV and maximum carbon sequestration for Europe were the basis for WGP, i.e. to minimize deviations. Therefore, our approach is equivalent to a compromise programming model on the *L* ~1~-norm. The WGP model is given by:$$\documentclass[12pt]{minimal}
                \usepackage{amsmath}
                \usepackage{wasysym}
   all countries and species with the respective maximum value, established through the management regimes yielding the highest NPV. Constraint equation ([6](#Equ6){ref-type=""}), similarly to constraint equation ([5](#Equ5){ref-type=""}), related the carbon sequestration deviations of all species and countries to its maximum value. Constraint equation ([7](#Equ7){ref-type=""}) assured that only one management regime is selected for each species and country. Constraint equation ([8](#Equ8){ref-type=""}) guarantees that the variables *x* ~*ijkl*~ assume only binary values.

The optimization model was solved multiple times, varying the weights for carbon sequestration and NPV from 0 to 1 in a 0.1 interval by using the software LINGO 13.0 on a PC with Intel® Core™ i5-3337U CPU, with 1.80 GHz and 4GB of memory.

Finally, the WGP optimization procedure was repeated to analyze the sensitivity of obtained optimization results to policy schemes


said on CBS’s “60 Minutes.”

“I am going to take care of everybody. I don’t care if it costs me votes or not. Everybody’s going to be taken care of much better than they’re taken care of now. … The government’s gonna pay for it. But we’re going to save so much money on the other side. But for the most it’s going to be a private plan, and people are going to be able to go out and negotiate great plans with lots of different competition with lots of competitors with great companies, and they can have their doctors, they can have plans, they can have everything.”

Trump presidential rival Sen. Ted Cruz Rafael (Ted) Edward CruzLoeffler calls for hearing in wake of Netflix's 'Cuties' Health care in theconditions

While he’s no fan of -ObamaCare, Trump agrees with politicians who want to keep the -ObamaCare rule that prevents insurance companies from denying coverage to or raising rates on patients with pre-existing conditions.

“Except pre-existing conditions, I would absolutely get rid of -ObamaCare. We’re going to have something much better, but pre-existing conditions ... I want to keep pre-existing conditions. I think we need it, I think it’s a modern age, and I think we have to have it,” Trump said during the CNN-Telemundo debate in February of 2016.

It’s a politically smart position, as a new CNN poll found that 87 percent of Americans back keeping the policy in place.

-ObamaCare’s individual mandate was meant to force enough young people into insurance plans to subsidize more expensive insurance customers, including


would a traditional retailer. Different methods of valuation are permissible to achieve a just and uniform valuation. Clark v. Vandalia R. Co. (1909), 172 Ind. 409, 86 N.E. 851.
However, nothing in the record substantiates the Board's argument that application of Regulation 16 resulted in uniformity between Pioneer and its retail competitors. In oral argument, the Board stated that the retail level of trade provisions in Regulation 16 are based on a general perception that as goods travel from producer, to dealer, to retailer they become more valuable. While this general perception may have a theoretical justification, no evidence was introduced at trial revealing what the valuation of similar inventory owned by a retailer would be. Neither this court nor the trial court could compare the tax liability ofuniform and equal nor a just valuation for all property.
"The Board has not attempted to show that its method of classification and valuation is required to achieve a just valuation upon all property, but only argues that its basis of valuation was uniform. It asserts that actual cost was used as the basis of assessment for all wheat and that the difference in value results from a difference in cost to the respective owners. The Constitution of Indiana does not require a uniform method of valuation, but rather such regulations as shall secure a just valuation for taxation of all property. [Original emphasis.] [Citation omitted.] While it is true that the relevant statutes [footnote omitted] recognize cost as a factor to consider in arriving at a just valuation,


and equality in tax burden do not occur unless identical property is assessed at the same tax value. [Emphasis supplied.]"
Lyon & Greenleaf at 277-78, 359 N.E.2d at 934-35.
Lyon & Greenleaf is indistinguishable from the case at bar. In both cases the Board has failed to show the classification of the same property which yields disproportionate tax liability is required to achieve a just valuation of all property. Inventory stored at Pioneer's facilities is distinguished from the same inventory in the hands of Pioneer's sales representatives. Such a distinction is probably more artificial than in Lyon & Greenleaf. In this case, not only is identical property valued differently but the same owner of identical property is taxed differently. We therefore conclude that the Board's higher valuation of Pioneer's seedwhen being confronted with certain minority groups is evident.

Biased results found by ACLU. (source)

Another underrepresented group in the machine learning community is women, and it too has discovered a problematic representation in a commonly used machine learning tool. Word embeddings are representations of the proximity of association of words within big corpus of text.

The two most popular pre-trained word embeddings are Word2Vec and GloVe, trained on millions of wikipedia, google news articles and the like. And since they process language as it naturally occurs on the internet, they also show signs of gender bias as it occurs on the internet. Male names appear in much greater proximity to business and scientific professions, while female names in these models appear closer to teaching professions or simply homemaking related words.

Biased


the sheer scale of a decision making model makes its outputs much more influential than that of any single human. Thus, machine decisions are just of a higher stake.

It is also not clear if we can quantify or formalize questions of ethics that many have spent years philosophising over to no avail. In fact, if society does not accept the ‘what was is what will be’ assumption, can algorithms help us at all in reaching fair and just decisions? The answer is not a binary one. We should not accept all past examples as ground truth, but no one is suggesting to get rid of history all together as an aid in reasoning.

If history is helpful, so are models trained on historical examples, so long as their underlyingthe inaccurate story of Lynch’s heroics to draw a comparison to Bergdahl.

"If the heroics that the Pentagon made up about her (Lynch) didn't really happen, and they didn’t, maybe the U.S. special forces who rescued her and the other Americans … maybe they shouldn’t have bothered," Maddow said, sarcastically. "After all, maybe it was sort of their own screw up that got them ambushed and hurt and captured in the first place. Is that how we think about these things now?"

Some people who saw Maddow’s reporting questioned her assertion that "the Pentagon made up" Lynch’s heroism. The Pentagon is the building that houses the U.S. Defense Department and a quasi-official way to reference the Defense Department. Saying the Pentagon "made up" something is the equivalent of accusing George


Q:

css solution to hide div after x amount of seconds

Any way using css3 only to remove/hide the #a after say 90 seconds of page load ?

i would love to go from display:block to display:none if possible ?

A:

This is possible with CSS animation and the forwards property to pause the animation at 100%. The display property cannot be animated.
The element is given position: relative and then opacity: 0 and left: -9999px when it reaches 100%.  It will fade and then pull itself outside the viewport.
See browser support here - Compatible IE 10+ !
Here is a complete list of animated properties.
Here are three ways to pull the div outside of the viewport at 100%:

left: -9999px combined with position:animation: css 0s 3s forwards; }

@keyframes css  { to { visibility: hidden; } }
                    /* visibility / overflow: hidden; */

Wait for 3 seconds...


would otherwise be necessary. The result is hardware having a maximum of a few tenths of ports (e.g., 16 or 32), running at very high-speed (e.g., OC-192 at 10 Gigabits/second) and capable of handling the full traffic of all ports without any loss thanks to a sophisticated flow control put in place to manage the congestion.
It practice however, it remains very difficult to take advantage of the full performance of every port. Not all applications require all ports to be of that speed. On the contrary, many applications of switch fabrics, even though they are attempting to utilize the full throughput capacity of the switch, require that a much larger number of lower-speed ports be accommodated in a switching box instead. Switch fabrics are expensive hardware. When buildingto work all hours of the day to get things done (for
better or worse), and be more likely to stay with the startup life after
several failures.

An older team generally needs more of a salary for financial obligations
(especially those with a family, mortgage, etc), wouldn't be able to work as
many hours (especially those with a family), and might be able to survive a
failure or two, then give up and go back to a FT job somewhere because of
their already-established connections.

Whether you have the same mindset or not, this may be the mindset of many
investors in Silicon Valley. And perhaps journalists & industry bloggers too,
which would explain why the media here tends to emphasize younger cofounders.

~~~
jacquesm
The media emphasizes younger co-founders because it makes for a much better
story. Rags to riches


before you're 25 sounds so much better than the same
before you're 60.

~~~
_delirium
I think there's also a disproportionate media focus on the _really_ big
successes, which do seem to have had pretty young founders: Apple (21 & 25),
Microsoft (19), Google (24 & 25), Dell (19), Amazon (30), etc.

~~~
pelemele
Jobs biggest success came when he was over 45 and honestly, we are not talking
here about stuff like break-thru cancer cure, new kind of alternative energy
or similar. We still use the same network stack (TCP, 1974), the same web
markup language (HTML, 1990) and the same programming languages as our fathers
(C++, 1979, PHP, 1995, SQL 1987).

~~~
einhverfr
Well, we aren't using my grandmother's computer programming language much
(Fortran).... Thank goodness ;-) hahahahah

~~~
dgabriel
Actually, Fortran is pretty widely used in the sciences. If you're working
with R, you're probably doingthat. A lot of solutions are to surprisingly obvious
problems that nobody ever saw before because we were accustomed to them.

------
ChuckMcM
I find this an interesting article in light of the previous "Oh shit I'm 30
and haven't done _anything_!" article.

There are young engineers who are too gullible and naive. Many VC's eat these
folks for lunch like Hollywood eats wannabe movies stars. I met a guy who said
that after his first startup he felt like some young kid who came out to act
and settled for a role in an adult film, only to have all the proceeds go to
the producer and a reputation sullied by is previous 'history.' His startup
had a good exit, for the VCs but was considered 'poorly executed' so he took
the heat.

I've met older engineers who were amazing


served by having no health coverage. With only 10 employees, we
don't qualify for anything but a low-tier HMO, and apparently risk pooling
(i.e. business-association group plans) is no longer allowed in MA.

I think the individual plans might be a worse deal for families, though, which
is of course a big consideration - I have no dependents.

------
johnohara
Lots of cross-currents in this article.

The premise focuses on "internet founders" which invariably celebrates
software development over hardware development.

Okay. But the truth is, an enormous amount of innovation continues on the
hardware side, and those development teams and founders are not generally
under 25.

Heck, if MSL Curiosity suddenly sputters on Mars it will probably be due to a
gray hair making contact somewhere.

------
trotsky
Much easier to bully your way into a shot caller role as a VC if your
portfolio companiesb) -2/7  (c) q
c
Let d = 0.7 + 0.5. Let x = -0.7 + d. What is the smallest value in 4, 1/3, x?
1/3
Let t = -7.7 - -8. Let n = t - 0.5. Which is the second biggest value?  (a) -2  (b) 0.1  (c) n
c
Let k be 2/9 + (-342)/810. What is the second biggest value in 0.2, k, -0.09?
-0.09
Let r = -11 + 16. Let v = r + -9. What is the third smallest value in 0.4, -5, v?
0.4
Let b = 16 - 16.3. What is the third biggest value in -0.1, b, -2?
-2
Suppose 11 + 1 = -4*d. What is the second smallest value in 0.07, d, -2/13?
-2/13
Suppose 0 = 43*w - 48*w. What is the biggest value in


2*l. Let a be (42/(-15))/(-1) + q. What is the second biggest value in 1/6, -2, a?
a
Let d be -2 + 4/2 - 0. Let q = 4 - 7/2. Let z = q + d. Which is the second biggest value?  (a) 5  (b) z  (c) 4/3
c
Let r be (-6)/16 - 33/(-44). Which is the second biggest value?  (a) r  (b) -1  (c) 1
a
Let m be 100/56 + 2/(-7). Let f = -45 + 134/3. Which is the biggest value?  (a) m  (b) f  (c) -5
a
Let h be (-6)/9 + (-7)/((-42)/(-20)). What is the second smallest value in h, 9, 3/5?
3/5
Suppose 0 = 4*d - 81 - 143. Let c be (16/d)/((-2)/(-3401)). Let h = -485 + c.extended periods of time (see Fig. \[fig:camp\]).

![Top: Configuration of the 4 MMS spacecraft and the flow speed direction during the MMS turbulence campaign. Bottom: Location of the MMS, along with the nominal magnetopause and bow-shock locations. The Geocentric Solar Ecliptic (GSE) [@Franz2002PSS] coordinate system is used, in which the XY-plane is defined by the Earth mean ecliptic and the +X-axis is defined by the Earth-Sun vector.[]{data-label="fig:camp"}](camp.png "fig:"){width="\linewidth"} ![Top: Configuration of the 4 MMS spacecraft and the flow speed direction during the MMS turbulence campaign. Bottom: Location of the MMS, along with the nominal magnetopause and bow-shock locations. The Geocentric Solar Ecliptic (GSE) [@Franz2002PSS] coordinate system is used, in which the XY-plane is defined by the Earth mean ecliptic and the +X-axis is defined by the Earth-Sun vector.[]{data-label="fig:camp"}](orb.pdf "fig:"){width="0.8\linewidth"}

During the first mini


wind. A schematic configuration of the four MMS spacecraft in the solar wind, during the Turbulence Campaign, is provided in the top panel of Fig. \[fig:camp\]. The orbital context plot showing MMS location relative to the nominal magnetopause [@Shue1998JGR] and bow shock [@Farris1994JGR], is illustrated in the bottom panel of Fig. \[fig:camp\].

MMS Data {#sec:data}
========

![Time series of solar wind observations from 16:39:00 - 21:41:00 UTC on 24 Feb 2019. Top panel: GSE components ofthe MMS1/FGM magnetic field, second panel: Proton number density measured by MMS1 and Wind, third panel: X component of the proton velocity, in GSE coordinate system, measured by MMS1 and Wind, bottom panel: Proton temperature measured by MMS1 and Wind.[]{data-label="fig:data"}](20190224_overview.pdf){width="\linewidth"}

  ---------------------------- -----------------------------------------------------
  Solar-wind speed                  $V_{\mathrm{SW}}=$ 322 km s$^\mathrm{-1}$
  Correlation Length                 $\lambda_{\mathrm{c}}=3.2 \times 10^5$ km
  Ion inertial length                         $d_{\mathrm{i}}=$ 91 km
  Ion gyroradius                       $\rho_{\mathrm{i}}=$ 64$^*$ (150) km
  Electron inertial length                   $d_{\mathrm{e}}=$ 2.3 km
  Debye length


of solar wind turbulence [@Bruno2005LRSP].

The Fast Plasma Investigation (FPI) [@Pollock2016SSR] instrument measures proton and electron distribution functions and moments every 150ms and 30ms, respectively. Due to the limitations of the FPI instruments in the solar wind [@Bandyopadhyay2018aApJ], some systematic uncertainties remain in the moments, and more so in the higher-order moments. [Therefore, we cross-check the proton moments in the selected interval with Wind [@Ogilvie1995SSR; @Lepping1995SSR] data, time-shifted to the bow-shock nose.]{} The MMS and Wind estimates of proton density, velocity (X$_{\mathrm{GSE}}$ component), and temperature are shown in the bottom three panels in Fig. \[fig:data\]. The density and velocity are in adequately close agreement, but significant discrepancies exist in the proton temperature values. The FPI estimates of temperature are significantly greater than the Wind values. Given the known limitations of FPI in the solarmagnetic field fluctuations using a multi-spacecraft technique, and taking advantage of a unique beads-on-a-string flight formation. The previous estimate by [@Matthaeus2005PRL], using Cluster data, is $\lambda_{\mathrm{T}}=2478$ km, which is about 3 times smaller than the present evaluation. The deviation is possibly due to the relatively larger spacecraft separation used in the Cluster data set, comparatively shorter intervals, and mixing of different solar wind intervals. It is also possible that this level of variability is intrinsic to the solar wind for a variety of reasons including $1/f$ noise, stream structure [@Matthaeus1986PRL]. [Another possibility is that the differences may be attributable to the differences in the formation of the Cluster and MMS spacecraft. The Cluster spacecraft pair baselines are in a tetrahedron, introducing anisotropy effects, which are not present in the


to depend on the turbulence cascade rate [@MattEA08-Taylor].

The present paper is a step in a broad progression of interplanetary measurements of fundamental plasma turbulence properties. The 1980 NASA Plasma Turbulence Explorer Panel emphasized the need for simultaneous multi-point measurements, in particular, plasma and magnetic field measurements, to make progress in this area [@Montgomery1980RNPTES]. More recently, the space plasma community has witnessed growing interest in understanding the multi-scale nature of turbulence processes in space, using multi-point measurements in observations [@Matthaeus2019WP; @Klein2019WP] as well as laboratory experiments [@Schaffner2014ApJ; @Schaffner2015ApJ], especially with regard to the physics of dissipation and heating mechanisms. The MMS turbulence campaign provides the first opportunity to capture multi-scale processes near the proton scales with a single data interval. Therefore, the results presented in this paper will be useful for futureABC ending its Live Well network

Robert ChannickTribune staff reporter

ABC is discontinuing its Live Well digital network, a national lifestyle channel with Chicago roots.

Live Well Network features 24/7 home, fashion, food and health shows, with offerings such as “Live Big with Ali Vincent,” and “My Family Recipe Rocks,” hosted by former 'N Sync member Joey Fatone. It airs on the digital subchannels of all 8 ABC owned and operated TV stations, including WLS-TV in Chicago, and reaches 64 percent of U.S. households, according to ABC.

The network will end its run by mid-January 2015, according to an email sent to affiliates Monday by Rebecca Campbell, president of the ABC owned stations, and Peggy Allen, vice president of Live Well Network. There was no word as to replacement programming for the


building materials, food, etc. (whatever the business you are talking to sells) It will help convince them if you can provide an estimate of the expected number of immigrants that will come if the rez becomes truly independent. If the businesses try to say that "they don't have much money to spend" then remind them that when thousands of new residents each spend a little, it quickly adds up to a lot. Tell business owners that placing signs out in front of their shop showing support for the RoL might make them look a bit better in the eyes of the RoL and help generate business. Tell them to use any political ties they have to convince the South Dakota gov. to grant independence to Pine Ridge andof laying out the child elements), regardless of the
  "display" property.
If you want to reliably have the children reliably arranged in a
  particular layout mode in a cross-browser fashion, your best bet is to
  use a wrapper-div inside the button, just as you would need to inside
  of a <table> or a <fieldset>.

https://bugzilla.mozilla.org/show_bug.cgi?id=984869


incubated with HRP conjugated secondary antibody (1:5000 in 5% milk, TBST) for 1 h at room temperature. After three TBST washes (10 min each), Pierce ECL western blotting substrate was added onto the membrane and incubated for 5 min to develop the chemiluminescent signal. Amersham Hyperfilm^TM^ ECL films were used to capture the intensity of the chemiluminescent signal. Exposed films were then developed in a Compact X4 automatic processor. The intensity of protein bands was quantified using the free ImageJ software available from <https://imagej.nih.gov/ij/>.

mRNA Purification, cDNA Synthesis, RT-PCR and qRT-PCR {#S2.SS7}
-----------------------------------------------------

Total mRNA was extracted using a miRNeasy Mini Kit (Qiagen, 217004) and first stand cDNA was synthesized with M-MLV reverse transcriptase (Promega, M368) using random hexamer primers (Promega, C1181). RT-PCR was performed in the G-Storm GS4M, qRT-PCR wasCTTGCGGTTACAGAGGCCAT     [NM_008355](NM_008355)         132
  Mouse GAPDH   AAGGTCATCCCAGAGCTGAA    CTGCTTCACCACCTTCTTGA     [XM_017321385](XM_017321385)   222
  Rat VPAC1     GCTCCTTAAAACTGGCCCCT    TCAAACACCTCAGTGCCGTT     [NM_012685](NM_012685)         149
  Rat VPAC2     GAAGGCAGAGAGGGCGATAG    CAACCAGCAGTAGCAGGTCA     [NM_017238](NM_017238)         152
  Rat PAC1      AGCATTCACCCCCTTTCCTCA   GGAGAGAAGGCGAATACTGTGT   [NM_001270579](NM_001270579)   175
  Rat MCP-1     CAGGTCTCTGTCACGCTTCT    GGCATTAACTGCATCTGGCTG    [NM_031530](NM_031530)         87


sciatic nerve as control. VPAC1, VPAC2, and PAC1 mRNA levels are all significantly up-regulated in the distal nerve stump following injury compared to their expression in control nerves ([Figure 1B](#F1){ref-type="fig"}). VPAC1 and VPAC2 show similar expression profiles and their expression becomes significantly elevated compared to control nerve at day 7 and peaks at day 10. PAC1 expression is significantly up-regulated at day 4 and peaks at day 10. Expression of VPAC1, VPAC2, and PAC1 begins to decrease at day 14 but remains significantly increased compared to control nerves ([Figure 1B](#F1){ref-type="fig"}).

Next, we investigated changes in protein expression by western blot in the proximal nerve stump and in the distal nerve stump at 7, 10, and 14 days following transection injury. Consistent with their mRNA up-regulation in the distal nerveof VPAC1, VPAC2, and PAC1 with macrophage marker F4/80 on transverse sections from mouse distal sciatic nerve at 7 days post-transection injury. **(J--R)** Double staining of VPAC1, VPAC2, and PAC1 with macrophage marker CD68 on transverse sections from mouse distal sciatic nerve 7 days post-transection injury. VPAC1 **(A--C,J--L)** and PAC1 **(G--I,P--R)** are expressed in most macrophages of the distal nerve stump. Macrophages expressing VPAC2 are indicated by arrows in **(D--F,M--O)**. Scale bars 20 μm. Three mice were used and three sections from each mouse were stained for each receptor.](fnins-13-01326-g005){#F5}

VIP and PACAP Increase Myelin Protein Expression in Cultured Schwann Cells {#S3.SS4}
--------------------------------------------------------------------------

It has been previously been described that VIP and PACAP treatment increases myelin protein expression in the rat RT4 schwannoma cell line ([@B6]), but this has not been tested


acting through to increase IL4, IL10, and IL13 expression in the nerve explants, we incubated the nerve explants with receptor specific agonists and then measured the levels of IL4, IL10, and IL13 expression. We found that VPAC1 and VPAC2 activation significantly up-regulated the expression of IL4, IL10, and IL13. However, PAC1 stimulation only up-regulated IL13, it had no effect on IL10 and IL4 expression ([Figure 8C](#F8){ref-type="fig"}), indicating that VPAC1 and VPAC2 are the major receptors increasing anti-inflammatory expression in the distal nerve explants.

Discussion {#S4}
==========

Vasoactive Intestinal Peptide and PACAP are regeneration-associated neuropeptides that are up-regulated in motor, sensory, and sympathetic neurons following peripheral nerve injury ([@B41]; [@B2]; [@B32]). Their up-regulation in motor neurons is detectable 6 h after peripheral nerve injury and both reach more than a 20-fold peakthe sciatic nerve explants and promote pro-inflammatory cytokine release. About 8% cells in the peripheral nerves are known to be resident macrophages and their activation contributes significantly to the early release of pro-inflammatory cytokine following peripheral nerve injury ([@B37]; [@B47]). We showed that macrophages in the mouse sciatic nerve express PAC1 the receptor ([Figure 5](#F5){ref-type="fig"}), thus, PACAP peaking at day 2 following injury may have an important function by acting on resident macrophages to promote early pro-inflammatory cytokine production. In contrast, the peak expression of VIP on day 7 following peripheral nerve injury well matches with the time point of pro-inflammatory cytokine down-regulation. Therefore, VIP could have more a important function than PACAP in terms of the resolution of the distal nerve inflammatory response.

Taken together, in this study,


the goods, the bads, the highs and the lows of 5 years of living away, as a small family, in little apartments, moving, sometimes a year later, and sometimes 4 months later. . .

but just know,

that I slept on an air mattress for a year when I lived in Indiana, and a bit in North Carolina.

(this was the living space. It was empty.)

A typical work day for my husband was leaving at 6:30 a.m., and typically arriving home at 8:00 p.m. We had one car, and he took it with him 98% of the time (long story). This meant I got to be in our little apartment, with 2 (or 3) little boys, all day, 6 days a week, sometimes 7 days a week. No friends. At first,tonight, the night before you turn 5 months old, my eyes filled with tears. I'm not talking about just a few tears. No, more like a constant hot flow streaming down my face, only to be followed by more with every blink.

Where these tears came from I'm not for certain. All I know is what I felt. I was overcome with emotion from a love for you that is impossible to describe. A love that I never want to change.

Your grandma (Gram) gave your daddy this book when you were born. She left a sweet note for him in the front cover:

Sugar Man,

Today with the birth of your son- your blessings have just begun. You are now feeling what I felt 26 years ago with the birth of


n B       # move cursor n lines down\n    ESC [ n C       # move cursor n characters forward\n    ESC [ n D       # move cursor n characters backward\n\n    # clear the screen\n    ESC [ mode J    # clear the screen\n\n    # clear the line\n    ESC [ mode K    # clear the line\n\nMultiple numeric params to the ``'m'`` command can be combined into a single\nsequence::\n\n    ESC [ 36 ; 45 ; 1 m     # bright cyan text on magenta background\n\nAllfor a valuable fix to stop ANSI chars being sent to non-tty\n  output.\n* Roger Binns, for many suggestions, valuable feedback, & bug reports.\n* Tim Golden for thought and much appreciated feedback on the initial idea.\n* User 'Zearin' for updates to the README file.\n* John Szakmeister for adding support for light colors\n* Charles Merriam for adding documentation to demos\n* Jurko for a fix on 64-bit Windows CPython2.5 w/o ctypes\n* Florian Bruhin for a fix when stdout or stderr are None\n* Thomas Weininger for fixing ValueError on Windows\n* Remi Rampin for better Github integration and fixes to the README file\n* Simeon Visser for closing a file handle using 'with' and updating classifiers\n  to include Python 3.3 and 3.4\n* Andy Neff for fixing RESET of LIGHT_EX colors.\n* Jonathan Hartley


the start time it will display ERROR in the Status field.

	.PARAMETER ComputerName
		The name of the computer you'd like to run this function against. By default, it will run against the local computer.
		You may pass multiple names to this delimited by a comma.  You cannot include wildcards. You may also pass computer names
		to this property from the pipeline.

	.INPUTS
		String. You can pass computer name via the $ComputerName parameter to Get-Uptime.

	.OUTPUTS
		System.Management.Automation.PSCustomObject
	#>
	[OutputType('System.Management.Automation.PSCustomObject')]
	[CmdletBinding()]
	param
	(
		[Parameter(ValueFromPipeline)]
		[string[]]$ComputerName = $env:COMPUTERNAME
	)
	begin ## Use a begin block to only process this code one time if names passed from the pipeline
	{
		$today = Get-Date ## Get the current date/time in the begin block to prevent executing Get-Date numerous times
	}
	process
	{
		foreach ($computer in $ComputerName) ## ComputerName is a string collection so we must be able to process each object
		{
			try ## Wrap all codelooking for trucks carrying foreign meat and milk products. (AP Photo/Christian Lutz)

Migrants from Syria and Afghanistan arrive on an overcrowded dinghy from the Turkish coasts to the Greek island of Lesbos. Nearly 50,000 people have illegally entered the country this year, mostly Syrian refugees. (AP Photo/Santi Palacios)

Residents look at a burnt-out house in Sant Salvador de Guardiona near Igualada. A wildfire raging in Spain's northeastern region of Catalonia threatens some 1.000 hectares of forest and farm land, forcing the evacuation of some 800 residents. (AFP PHOTO/JOSEP LAGO)

Team France competes in the Women's Team Technical final during the synchronised swimming competition at the 2015 FINA World Championships in Kazan. (AFP PHOTO/CHRISTOPHE SIMON)

Muhyiddin Yassin (C) waves to journalists as he arrives before a press conference at his residence in Kuala