Site-directed mutagenesis of the serotonin 5-hydroxytrypamine2 receptor: identification of amino acids necessary for ligand binding and receptor activation.
Serotonin 5-hydroxytryptamine (5-HT)2 receptors are implicated in the etiology of mental disease and depression. Drugs that interact with the 5-HT2 receptor are used therapeutically to treat such illnesses, and their mechanisms of action are of great interest. In this study 5-HT2 receptor-ligand interactions were examined by site-directed mutagenesis in which three aspartic acid to asparagine mutants (Asn-120, Asn-155, and Asn-172) were created and expressed in NIH3T3 cells. The Asp-120 to asparagine mutant exhibited the same affinity for 125I-lysergic acid diethylamide (125I-LSD) as did the wild-type receptor and showed a decreased and GTP-insensitive binding affinity for the agonists 5-HT and (+/-)-1-(2,5-dimethoxy-4-iodophenyl)-2-amino-propane (approximately 10-fold) and the antagonists ketanserin and mianserin (approximately 10-fold) butone – the amount of traffic on each street. And what Donald Appleyard was able to show that just the mere presence of cars with the envelope of danger that they project around them, and the noise and the pollution, crushes the quality of life in our neighbourhoods.

Bruce Appleyard: [00:48] What’s really interesting about the graphics in Livable Streets and what they really provided was it removed us from just looking at specific numbers of people being hit or killed on streets, but actually showing that there was this other way that we could measure the environmental impacts of traffic on neighbourhood streets. Things such as a light traffic street helped knit a community together,


lots of details, lots of nuances. These studies were the first piece of academic research in the United States to document the social harm that is done by traffic.

Bruce Appleyard: [05:38] Auto ownership in this country has grown threefold since livable streets was first published. So traffic conflicting an incursion on neighbourhoods is still a major issue we need to deal with.

[music]

 Transcript Divas Transcription Canada Nuclear Accidents continues with Three Mile Island, the Kyshtym Disaster, the Fernald Feed contamination, the SL-1 meltdown, the Soviet sub K-431 refueling disaster, the Zaragoza radiotherapy accident, Chernobyl and Fukushima, plus news and pop culture. Part 2 of 2 with special guespert Bob Apthorpe


of 3--5 were classified as susceptible (S) \[[@pone.0126130.ref034]\]. The RF of the accessions in relation to the isolates of *M*. *oryzae* could be calculated according to the following formula: RF = (the number of incompatible isolates / the total number of isolates used) \*100%. Each RF value was assessed from two technical replicates.

DNA markers and genotyping of 277 rice germplasms {#sec005}
-------------------------------------------------

A total of 15 FMs and 9 LMs were selected from published primer sequences to detect 18 *R* genes, including six alleles of the Piz locus (*Piz*, *Pizt*, *Pi2*, *Pi9*, *Pi40* and *Pigm*) \[[@pone.0126130.ref035], [@pone.0126130.ref036]\], two alleles of the Pik locus (*Pik* and *Pi1*) \[[@pone.0126130.ref010], [@pone.0126130.ref037]\] and ten independent *R* genes (*Pia*, *Pib*, *Pit*, *Pish*, *Pi5*, *Pi54*, *Pita*, *Pid2*, *Pid3*, and *Pb1*) \[[@pone.0126130.ref006]\] in the 277 accessions. Detailed*indica* backgrounds, and *Pita* only played a major role in *japonica* backgrounds ([Table 2](#pone.0126130.t002){ref-type="table"}). These results indicated that some *R* genes like *Pita* and *Pi54* may be exhibiting a great interaction effect with the *indica*-*japonica* genome background. Therefore, the genetic background must be considered when using these *R* genes for breeding cultivars with broad-spectrum resistance.

It is thought to be a useful strategy to pyramid several *R* genes for breeding cultivars with broad-spectrum and durable resistance. However, random pyramiding of various *R* genes may not show an additive effect, as indicated by the combination of the *Piz5* and *Pita* genes in Co39 \[[@pone.0126130.ref045]\]. He et al. \[[@pone.0126130.ref046]\] also reported that the resistance effect of pyramiding lines with *Pi4* and *Pi1* was weaker than that in lines with the single


/-
Copyright (c) 2019 Microsoft Corporation. All rights reserved.
Released under Apache 2.0 license as described in the file LICENSE.
Authors: Leonardo de Moura
-/
prelude
import Init.Data.Array.Basic
universes u v

namespace Array
-- TODO: remove the [Inhabited α] parameters as soon as we have the tactic framework for automating proof generation and using Array.fget
-- TODO: remove `partial` using well-founded recursion

@[specialize] partial def binSearchAux {α : Type u} {β : Type v} [Inhabited α] [Inhabited β] (lt : α → α → Bool) (found : Option α → β) (as : Array α) (k : α) : Nat → Nat → β
| lo, hi =>
  if lo <= hi then
    let m := (lo + hi)/2;
    let a := as.get! m;
    if lt a k then binSearchAuxperformances, such as response time, accuracy of position and stability. In addition the conventional fluid-powered rotary actuation systems have many unsolved problems and disadvantages, such as heavy weight, high leakage, slow response, low accuracy, extra adapters, tubes and brackets for unmatched interfaces, lower reliability and lack of intrinsic safety features.
The most conventional rotary actuators are based on two types of linear-rotary movement conversion mechanisms; scotch-yoke and rack-pinion. They all have a common problem; unbalanced side loading forces, either on linear movement side or rotary movement side. As a result, the actuators have lower efficiency, heavy weight, high friction and high maintenance cost and are expensive to produce, and those problems largely prevent the actuators from being used in precision flow controls, fast operations or critical applications on medical


of millions of middle-class Americans, she can always find comfort in the stupendous, fantastic, if just a little fabricated rating of her book on Amazon, which as of today has 4.8 completely unhacked stars out of 5... with Jeff Bezos' sincerest compliments.Be Vulnerable for God

Esther, “Why is it so easy for you to talk about your past with masturbation and pornography?” asked someone.

“It’s not easy. However, I choose to be vulnerable, embarrassed and called whatever names all for the glory of God, for Jesus and for someone in this room or out there who will get blessed by my past & testimony” I answered.

As much as I will like to keep my past away or forget it. The Holy Spirit says no. I could be at a gathering and He will say, “Oh, Esther you gotta share your testimony now. Someone needs to hear it right now.” And at that point in time, nothing else matters. My feelings don’t matter. Of course, the devil tries to play his schemes


or how you feel, it’s all about God, his glory and someone’s life that could change right now.”

So, I choose to be vulnerable for God.

But the amazing thing is, when you allow God to be glorified through you, He takes care of you and doesn’t disregard your feelings. What do I mean? When I first shared my testimony, I felt tremendously free. I no longer have to pretend, cover my weakness or put up a facade. However, I constantly realize that there are different degrees of freedom, because the more I share my story, the freer I get and the devil is shamed.

Because now, the devil can’t come to my face and try to say thing like, “Esther, you are such as bad girl and you don’t wantcompleted. Once septation is complete, daughter cells secrete degradative enzymes to destroy the primary septum and release daughter cells from their mothers ([Fig. 1 A](#fig1){ref-type="fig"} i). A "hippo" signaling pathway called the regulation of Ace2 and morphogenesis (RAM) network controls this process (reviewed in [@bib100]). Mutants in this pathway exhibit defects in cell separation and accumulate as large clumps of cells ([@bib101]; [@bib68]; [@bib47]). The downstream most kinase, Cbk1, is critical to direct the cell separation process ([@bib8]; [@bib16]; [@bib101]; [@bib39]). In late mitosis, Cbk1 is activated upon MEN-dependent release of the phosphatase Cdc14 and activates the transcription factor Ace2 ([@bib9]). Cbk1 interacts with Ace2 through a recently discovered docking motif ([@bib70]; [@bib28]) and phosphorylates the transcription factor Ace2 at its nuclear export sequence, trapping the protein in


analysis. We saw no difference in budding index, *CTS1* transcription, translation, or secretion in the presence or absence of auxin in control experiments with WT cells (Fig. S1, C--E). Next, we examined potential regulation of Cts1 during a single cell division event in which cytokinesis was disrupted. Use of the *inn1-AID* genotype allowed us to directly compare the same population of cells with normal (without auxin) or failed septation (with auxin). We observed identical induction of *CTS1* transcription following mitotic exit in cells treated with or without auxin, 0--60 min following release ([Fig. 2 A](#fig2){ref-type="fig"}), demonstrating that regulation occurs normally downstream of the transcription factor Ace2.

![**Septation failure is associated with a block in secretion of the septum degrading enzyme Cts1. (A)** Cts1 transcript induction is not altered uponleading to death. Therefore, if the lethal interaction of *FIR1* and *inn1-AID* is due to premature cell separation, it should be suppressed by *CTS1* deletion. We found that *CTS1* deletion restored viability to *inn1-AID fir1*Δ cells treated with auxin ([Fig. 3 B](#fig3){ref-type="fig"}). These data support a role for *FIR1* as a regulatory component of a system to monitor septum completion, and that suggests its absence leads to bypass of this system.

Fir1 prevents Cts1 secretion in septation mutants to ensure cell integrity {#s06}
--------------------------------------------------------------------------

A block in Cts1 secretion following failure to complete septation ([Fig. 2](#fig2){ref-type="fig"}) and genetic evidence demonstrating the lethality of *inn1-AID fir1*Δ cells is reversed by elimination of *CTS1* ([Fig. 3 B](#fig3){ref-type="fig"}) suggest that Fir1 functions to block inappropriate Cts1 secretion. To test this directly, we examined Cts1's


production of Cts1 began around 40 min in both auxin-treated and untreated cells and increased over the time course of the experiment. We also did not observe any evidence in overall glycosylation pattern, with a similar smearing and banding pattern in the presence or absence of *FIR1* (compare [Fig. 2 B](#fig2){ref-type="fig"} and [Fig. 3 D](#fig3){ref-type="fig"}).

However, we saw a marked difference in Cts1 secretion in cells lacking *FIR1*. Unlike *inn1-AID* cells ([Fig. 2, B and C](#fig2){ref-type="fig"}), which exhibit reduced Cts1 secretion, auxin-treated *inn1-AID fir1*Δ cells secreted Cts1 to the media at levels comparable to mock-treated cells ([Fig. 3, D and E](#fig3){ref-type="fig"}). Interestingly, in the absence of auxin, secreted Cts1 levels began to increase at 60 min (compared with 75 min) in cells lacking *FIR1* compared with WT cells (compareWhile this strain expresses *CTS1* to a lower level than WT *ACE2*, *CTS1* transcripts are produced with similar timing and levels in both *ACE2-GOF* and *ACE2-GOF cbk1*Δ (Fig. S5 A). We next assessed the production and secretion of Cts1 protein following release from mitosis in the presence and absence of Cbk1. We found that WT and *cbk1Δ* cells carrying the ACE2-GOF allele had no difference in the production of intracellular Cts1 protein ([Fig. 8, B and C](#fig8){ref-type="fig"}). However, *cbk1Δ* cells had consistently reduced secretion of Cts1 to the media ([Fig. 8, B and C](#fig8){ref-type="fig"}). This result suggests a novel Cbk1 function to promote Cts1 secretion independent of its role in promoting the transcription and translation of *CTS1*.

![**Cbk1 promotes Cts1 secretion independent of *CTS1* transcription. (A)** Schematic of the


*ACE2*-GOF allele bypassing the necessity of Cbk1 to activate *CTS1* transcription. The *ACE2*-GOF allele mimics Cbk1 phosphorylation at S122, S137, and S436 to promote *CTS1* expression in the absence of *CBK1*. **(B)** Cts1 secretion is reduced in the absence of *CBK1* despite normal Cts1 production. WT, *cbk1*Δ, *ACE2*-GOF, *ACE2*-GOF *cbk1*Δ cells expressing HA-tagged Cts1 were synchronized in mitosis. Protein was collected at the indicated times following mitotic release at 30°C and were processed as in [Fig. 2 A](#fig2){ref-type="fig"} (see also Fig. S5 A). **(C)** Western blot quantification (as in [Fig. 2 B](#fig2){ref-type="fig"}). Fold change relative to the maximum signal of WT cells is shown. **(D)** Fir1 primarily functions to inhibit Cbk1 to prevent Cts1 secretion. *inn1-AID ACE2*-GOF cells with the additional genotype indicated to the right of thethe Rho1 GTPase required for SS formation (late; [@bib77]). However, we propose here a different mechanism to ensure order. Unlike these other factors, Fir1 does not appear to be required for any step of septation or cell separation. Cells lacking *FIR1* have no obvious phenotype. Instead, Fir1, like other checkpoint proteins, functions to relay information about the status of an early event to a late event. Upon septation failure, Fir1 is stabilized and transmits this information to the RAM network, preventing later processes.

Checkpoints function by monitoring a process and sending a negative signal to block subsequent processes until a particular condition is satisfied. What is the signal that communicates the status of cytokinesis to Fir1? In ECO, we do not yet understand which aspect of septation is being


on a 10% SDS-PAGE gel and subjected to immunoblotting as indicated below.

IP {#s19}
--

We prepared cell lysate by bead-beating in ice-cold lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 10% glycerol, 1 mM dithiothreitol, 120 mM β-glycerolphosphate, 2 mM sodium orthovanadate, 20 mM sodium molybdate, 3 mM benzamidine, 1 mM phenylmehtylsulfonyl fluoride, 1 µg/ml pepstatin, 0.5 mM leupeptin, and 2 µg/ml chymostatin). Anti-HA antibody (12CA5, a gift from R. Lamb) at a 1:50 dilution along with 50 µl of a 1:1 recombinant protein G-Sepharose (Invitrogen) slurry was added to normalized protein, as determined by Bradford assay (Bio-Rad). We rotated IPs at 4°C for 2 h followed by two washes in ice-cold yeast lysis buffer. We then resuspended the beads in 2× SDS-PAGE sample bufferR. Lamb) and recombinant protein G-Sepharose (Invitrogen) slurry to determine the total Fir1-HA in the lysate. The other aliquot was incubated with anti-Cbk1 NT5 antibody ([@bib39]) and recombinant protein G-Sepharose (Invitrogen) slurry for the co-IP. We rotated the beads at 4°C for 2 h followed by three washes in ice-cold yeast lysis buffer. We then resuspended the beads in 2× SDS-PAGE sample buffer (125 mM Tris, pH 6.8, 20% glycerol, 4% SDS, 10% 2-mercaptoethanol, and 0.004% bromophenol blue) and resolved the samples on 8% SDS-PAGE gels. Gels were immunoblotted as described below.

Immunoblotting {#s21}
--------------

For all Western blot assays, we transferred proteins to Immobilon FL polyvinylidene difluoride (Millipore) membrane. Membranes were blocked for 30 min in Odyssey blocking buffer PBS (Licor), then incubated with primary antibody in Tris-buffered saline (50


was plotted. The mean number of cells per clump is indicated as a red line in the figure. One-way ANOVA with Tukey's multiple comparison test was performed using GraphPad Prism version 5.03. Data distribution was assumed to be normal, but this was not formally tested. For zygote-like morphology analysis, cells were arrested in mitosis and released with the addition of 0.5 mM auxin (in DMSO) or DMSO. Cells were grown for an additional 2.5--3 h to allow bud growth, and morphology was binned as chained or zygote-like. Cells were analyzed from three independent experiments in which *n* \> 100 cells were counted for each genotype. Significance was determined with an unpaired, two-tailed *t* test using GraphPad Prism version 5.03.

Chitinase enzymatic assay {#s25}
-------------------------

We washed metaphase-arrested cells three times inDisk Confocal system as indicated below. Images of GFP and/or mCherry fused proteins were acquired at room temperature in synthetic minimal medium. We took images in [Fig. 5 (A and C)](#fig5){ref-type="fig"}, Fig. S1 (A and B), Fig. S3 (B and C), and Fig. S4 A on an Axiovert 200 M microscope fitted with a 100×/1.45 NA oil immersion objective and Cascade II-512B camera (PhotoMetrics, Inc.). Images were acquired using Openlab software (v5.5.0; Improvision), and FIJI and Photoshop (Adobe) were used to make linear adjustments to brightness and contrast. We used the DeltaVision Core fit with a U PLAN S APO 100×, 1.4 NA objective (Olympus), and a CoolSnapHQ2 Camera (Photometrics) for images in [Fig. 6 E](#fig6){ref-type="fig"}. A z-series of 0.2-µm step size was taken at the indicated time


F. Cross, E. Bi, R. Lamb, Y. Barral, and S. Strahl for strains, plasmids, and antibodies. We thank C. Wilke (Northwestern University, Biological Imaging Facility) for assistance with electron microscopy and E. Anderson and S. Zdraljevic (Northwestern University) for assistance with R software (statistical analysis and graphing). We also thank staff and instrumentation assistance from Northwestern University's Biological Imaging Facility, which is supported by the Chemistry for Life Processes Institute and the Northwestern Office for Research, and from the Northwestern University's High Throughput Analysis Laboratory, which is a core facility of the Lurie Cancer Center (grant \#P30CA060553) .

Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under award number R01GM084223 to E.L. Weiss. The content ismandates that require
isolation of any ambient capture devices.

In the alternative, the ability to flood and overload such circuits might be
of interest.

------
orbitingpluto
Shazam keeping the microphone on at all times on the Mac shouldn't be a
surprise considering that it is already well known that Shazam has been
keeping the microphone on at all times on the iOS platform.

(sorry for the forbes link:
[http://www.forbes.com/sites/katherynthayer/2013/12/19/the-
al...](http://www.forbes.com/sites/katherynthayer/2013/12/19/the-always-on-
auto-shazam/#30088907b787) )

~~~
zimpenfish
You have to specifically enable that and you get the big red status bar that
tells you the microphone is on.

Unless there's a way for an app to use the microphone without that appearing?

------
nkrisc
On the radio today I heard a commercial instruct listeners to "Shazam this ad"
to learn more about whatever it was they were selling. That commercial angle
for Shazam is fine, but it makes the fact they keep your


four were dressed in light jackets, sweat shirts and sneakers.

"Next time I go out there, I won't be a knucklehead," Dominguez said. "I'll have some boots on."

His son Christopher told CNN that the family camps out during the summer, but wryly added: "The way we go camping isn't like what we did over the last few days."

By Wednesday morning, at least 45 search-and-rescue specialists from all over Northern California were combing the steep hills and canyons in the rugged area near where the family's truck had been discovered. For a while, search dogs were out, but snow that was 2 to 6 feet deep limited their effectiveness. Rescuers crisscrossed the area in snowmobiles, and dozens of additional searchers were converging from as far as Oregon.

Early in the afternoon,a CHP helicopter piloted by Steve Ward was churning through canyons north of where the search was being conducted on the ground. With another storm coming in, Ward and paramedic David White were about to return to their base in Auburn when they saw a man frantically waving at them. He was two to three miles from the parked pickup truck, officials said.

At first they thought he might be one of the searchers -- but then they saw the message spelled out in branches.

"This was our last pass," Ward said. "We were very lucky that we saw this guy."

After the chopper touched down, White and Ward helped the children climb up to the copter from their culvert beneath a fire road, trudging through snow 2 1/2 feet deep.


bought is about 3 feet wide and holds about 100 lbs at a time. Just load onto the ATV racks as many bags as it can handle and drive around stopping to fill up whenever the spreader is empty.

The pulverized lime goes for $2.49 a 50 lb bag while the pellitized lime was $3.49 for a 40lb bag.

Jeff

knobby

09-05-2007, 07:55 AM

It will basically turn to concrete.

amen to that....it is still on the spreader wheels

I honestly didn't know what I was buying...I was disappointed they were out of pellet lime. With my work schedule I had to get something accomplished so I tried the hydrated. I was pretty surprised how fine it was. It poured into the spreader almost like thick water.battle the Skaven, a subterranean race of ratmen, now allied with the Norscans, the Chaos-worshiping marauders from the frozen wastes of the north. While the addition of Norscans provides some much-needed visual variety, there’s not much difference in how you combat the rank-and-file enemies.

If you didn’t guess from the title, you spend a lot of time killing rats. Fatshark Games.

Returning from the original game are the cast of five heroes, such as the zealous witch hunter Victor Saltzpyre and the battle wizard Sienna Fuegonasus. New is the career system, in which each character has three classes, each with a unique look, active and passive ability, and talent tree. It’s a fun addition, but some careers are objectively better than others—I’d like to see them all be viable at


difficult to get a piece of equipment for the specific character you wanted, leaving you at the mercy of the random number generator. Vermintide 2 has remedied this by awarding loot boxes at the end of each successful match and every time you level up a character. Don’t worry, no microtransactions here. Each box awards three pieces of equipment, and whichever character you open the box with, you’re guaranteed at least one piece of equipment specific to that character. It’s a much better, though not perfect, system that encourages you attempt levels at higher difficulties for better chances at exotic loot. There are cosmetic items to unlock, but they seem to be extremely rare—it seems you have better odds of winning the Powerball than unlocking a new helmet.

Onefor reducing the immunogenicity of rodent antibodies involves the production of chimeric antibodies, in which mouse variable regions (Fv) were fused with human constant regions (Liu et al. (1987) Proc. Natl. Acad. Sci. USA 84:3439-43). However, mice injected with hybrids of human variable regions and mouse constant regions develop a strong anti-antibody response directed against the human variable region, suggesting that the retention of the entire rodent Fv region in such chimeric antibodies may still result in unwanted immunogenicity in patients.
Additionally, grafting of rodent complementarity determining region (CDR) loops of variable domains onto human frameworks (i.e., humanization) has been used to further minimize rodent sequences. Jones et al. (1986) Nature 321:522; Verhoeyen et al. (1988) Science 239:1534. However, CDR loop exchanges still do not uniformly result in an


antibody with the same binding properties as the antibody of origin. Changes in framework residues (FR), residues involved in CDR loop support, in humanized antibodies also are often required to preserve antigen binding affinity. Kabat et al. (1991) J. Immunol. 147:1709. While the use of CDR grafting and framework residue preservation in a number of humanized antibody constructs has been reported, it is difficult to predict if a particular sequence will result in the antibody with the desired binding, and sometimes biological, properties. See, e.g., Queen et al. (1989) Proc. Natl. Acad. Sci. USA 86:10029, Gorman et al. (1991) Proc. Natl. Acad. Sci. USA 88:4181, and Hodgson (1991) Biotechnology (NY) 9:421-5. Moreover, most prior studies used different human sequences for animal light and heavy variable sequences, rendering theSelf-driving shuttles are set to hit the streets of Northern Virginia in coming months, in what could just be the first of several deployments across the region.

Self-driving shuttles are set to hit the streets of Northern Virginia in coming months, in what could just be the first of several deployments across the region.

“We’re working with two particular pilots, both here in Northern Virginia, and I think….we will see a deployment of an autonomous shuttle in public transit operation in Northern Virginia before the end of this year,” said Jennifer DeBruhl, chief of public transportation with the Virginia Department of Rail and Public Transportation.

Though DeBruhl declined to detail the locations, Fairfax County is working with the state to launch an autonomous shuttle between the Dunn Loring-Merrifield Metro station and


This website uses cookies to ensure that we give you the best experience on our website. We also use cookies to remember your settings and gather web statistics. If you do not consent to cookies being sent to your device, please change your browser settings accordingly. If you agree to cookies being sent to your device please click “Continue”. Please note that you can change your browser cookie settings at any time, disabling some or all of them. For more detailed information about cookies we use, please see our “Cookie Policy”.1. Technical Field
Embodiments of the subject matter described in this specification relate generally to computer networks. Certain embodiments relate to techniques for providing routing information for routing packets to a destination node.
2. Background Art
In equal-cost multi-path (ECMP) routing techniques, multiple network paths to the same network node are identified as being associated with the same level of strategic cost (or value) in terms of route selection. Conventional ECMP techniques, such as those discussed in the Network Working Group's Request for Comments (RFC) 2991 (November, 2000) and RFC 2992 (November, 2000), are typically concerned with trying to send on each of the network paths identified as being of equal-cost the same amount of traffic to the destination node.
As data networks trend towards increased size, complexity and speed, mechanisms such


could think of to do to help her; he asked the Maesters to help her, demanded that they try harder, and finally cried to the Septon to pray for her. But nothing worked, and in the end Roy would find himself without his mother.

In the wake of Jeyne's death, Cassandra stepped up and began to fulfill her role in some ways, and Roy began to find that though he might be the next lord of Storm's End, that did not make him better than his siblings. Yet it would only be in 90 AC when Raymont, tired of his son's ego and lackluster nature for a future lord, sent him to be squired to Aemon Targaryen.

Squireship Edit

Roy, never one to do much more than show up to theway, he declared in a letter to his father "should you not rise in his name, I will personally take my halberd and deal with these men myself". Though it certainly didn't sway Raymont, who was already planning on joining with the crown, it did show to him that his son had grown from the pliable boy of his youth into someone ready to see battle.

And so, at the outbreak of the war, Raymont asked Aemon to knight Roy so that he may be at his side in the coming battles. Roy couldn't think of a prouder moment in his life than that day. After, he would follow his father into battle, joining with the Stormlander army gathering at Storm's End.

In the early days of the war it


men until a true battle occurred outside Mistwood, with men on both sides taking breaks from the fighting to loot the nearby town and pillage the countryside. Finally outraged enough to act, Roy gathered his men at Crow's Nest and began a guerrilla campaign against the men of Toyne. With the disorganized nature of the forces opposing him, as well as their better familiarity with the area, Roy struggled to get a conclusive victory, almost resolving to go to Toyne's keep itself and burn it to the ground before being talked out of it.

Lord Wylde, sensing a chance to escape the ire of Lord Baratheon, offered aid. Roy saw right through and shot that down. In response, Wylde desperately launched his fleet into the sea, alongside his levy.reached him, it became clear that there was no hope for any victory, only prolonging the inevitable. In rags, he came to the Baratheon camp alone and submitted. This act of humility calmed Roy's violent rage, and in exchange for a hostage he agreed to refrain from the slaughter of House Wylde as a whole.

After his victories, Roy would remain content in Storm's End, believing that he saw off the factionalist threat for the time being. As yet unwilling to be married, he nonetheless found a lover in his old friend Jenny Blackwood. Things looked bright for House Baratheon, and Roy himself.

Yet with Balerion's death, Roy has found cause to worry once more...

Current Events Edit


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Elections have consequences and from this dirty money came a compromised candidate who is beholden to the liberal elites.Passenger, loss, partial loss, theft or other damage, arising out of or in connection with carriage or other services incidental thereto performed by us.

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You will be required to pay a charge for carriage of Baggage in excess of the free Baggage


your Baggage:

8.4.1.1 items which are likely to endanger the aircraft or persons or property on board the aircraft, such as those specified in the International Civil Aviation Organisation (ICAO) Technical Instructions for the Safe Transport of Dangerous Goods by Air and the International Air Transport Association (IATA) Dangerous Goods Regulations, and in our regulations (further information is available from us on request);
8.4.1.2 items the carriage of which is prohibited by the applicable laws, regulations or orders of any state to be flown from or to;
8.4.1.3 items which are reasonably considered by us to be unsuitable for carriage because they are dangerous, unsafe or by reason of their weight, size, shape or character, or which are fragile or perishable having regard to, among other things, the type of aircraftcrated and accompanied by valid health and vaccination certificates, entry permits, and other documents required by countries of entry or transit failing which, they will not be accepted for carriage. Such carriage may be subject to additional conditions specified by us, which are available on request.

8.10.2. If accepted as Baggage, the animal, together with its container and food, shall not be included in your free Baggage allowance, but shall constitute excess baggage, for which you will be obliged to pay the applicable rate.

8.10.3. Guide dogs accompanying Passengers with disabilities will be carried free of charge in addition to the normal free baggage allowance, subject to conditions specified by us, which are available on request.

8.10.4. Where carriage is not subject to the liability rules of the Convention, we are


the person receiving the advance payment was not the person entitled to such compensation.

15.3. LIABILITY IN THE CASE OF CODESHARE FLIGHTS

In the case of flights operated as codeshare services in association with another carrier, we shall be liable to those passengers who have concluded a contract of carriage with us and for whom we are stated as the carrier in their ticket.

16. Compensation for denied boarding due to overbooking

16.1. DENIED BOARDING DUE TO OVERBOOKING

Should more passengers hold confirmed reservations for a particular flight than the capacity available, and should we have to refuse carriage to you as a result of such overbooking, you shall receive compensation in accordance with the provisions below, provided that:
(i) you hold a properly-issued ticket with a confirmed reservation and we or our agentsBest of Josh Richardson - Basketball Player

Best of Josh Richardson - Basketball Player

Knoxville, Tn - Mississippi State Bulldogs Vs Tennessee Volunteers

Collegiate Images

Guard Josh Richardson #1 of the Tennessee Volunteers during the game between the Mississippi State Bulldogs and the Tennessee Volunteers at Thompson-Boling Arena in Knoxville, TN. The Vols defeated the Bulldogs 72-57.

Guard Josh Richardson #1 of the Tennessee Volunteers during the game between the Mississippi State Bulldogs and the Tennessee Volunteers at Thompson-Boling Arena in Knoxville, TN. The Vols defeated the Bulldogs 72-57.


/*
 * Copyright (c) 2018, Oracle and/or its affiliates. All rights reserved.
 * DO NOT ALTER OR REMOVE COPYRIGHT NOTICES OR THIS FILE HEADER.
 *
 * This code is free software; you can redistribute it and/or modify it
 * under the terms of the GNU General Public License version 2 only, as
 * published by the Free Software Foundation.  Oracle designates this
 * particular file as subject to the "Classpath" exception as provided
 * by Oracle in the LICENSE file that accompanied this code.
 *
 * This code is distributed in the hope that it will be useful, but WITHOUT
 * ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or
 * FITNESS FOR A PARTICULAR PURPOSE.  See the GNU General Public License
 * version 2       value = runtimeArgs[++i];
                    }
                    if (opt.equals("--add-modules") && value.equals("ALL-DEFAULT")) {
                        // this option is only supported at run time;
                        // it is not required or supported at compile time


           super(parent);
            this.map = map;
        }

        @Override
        protected Class<?> findClass(String name) throws ClassNotFoundException {
            byte[] bytes = map.get(name);
            if (bytes == null) {
                throw new ClassNotFoundException(name);
            }
           [@pone.0059924-Kim1]. Family 1 are the largest GT family that has various potential substrates from plant secondary metabolism. The widespread occurrence, diversity and complexity of the glycosides throughout the plant kingdom indicate that Family 1 GTs might have broad functionality. Although some UGTs have been chemically characterized, very little is known about their actual biological functions and molecular mechanisms, particularly during plant growth, development and response to external environments.

In this study, a novel Arabidopsis GT gene *UGT85A5*, a group G member of Family 1, was identified as involved in regulating plant salt stress tolerance. The expression of *UGT85A5* was strongly induced by NaCl. Ectopic expression of *UGT85A5* in tobacco resulted in enhanced salt tolerance in transgenic compared to wild type (WT) plants. The data presented here demonstrate, for the


abiotic stresses [@pone.0059924-Parvanova1], [@pone.0059924-Bartels1]. Therefore, to gain further insight into the physiological relevance of *UGT85A5*, we analyzed changes of these biochemical markers in transgenic and WT plants under salt stress conditions.

Under normal conditions, a similar proline level was detected between WT and transgenic lines. Under salt stress, however, there were 17- and 16-fold higher proline levels in T~1~ and T~3~ transgenic plants, respectively. There was only a 12-fold increase of proline level in WT plants ([Figure 6A](#pone-0059924-g006){ref-type="fig"}).

![Changes in soluble osmolyte content, lipid peroxidation and Na^+^/K^+^ ratio of the WT and transgenic lines under salt stress.\
Leaves from plants treated with salt stress were collected and subjected to the determination. (A) Proline content; (B) Soluble sugars content; (C) MDA content; (D) Na^+^/K^+^ ratio.](pone.0059924.g006){#pone-0059924-g006}

A similar result was obtained in content of[@pone.0059924-Lawlor1]. The leaf disk assay in this study showed less chlorophyll loss in transgenic than in WT plants under salt stress conditions, indicating higher photosynthetic capacity in *UGT85A5* transgenic than in WT plants.

Organic osmolytes such as proline and soluble sugars work in a variety of ways, such as in protection of cellular structures, detoxification of enzymes and scavenging of ROS alone or in combination with other defense-related enzyme systems [@pone.0059924-Verma1], [@pone.0059924-Xiong1]. These compounds also confer integrity to the membranes and keep the photosynthetic system functioning [@pone.0059924-Xiong1], which is in agreement with our data showing higher chlorophyll contents in transgenic than in WT plants. The plasmalemma is known to play critical roles in perceiving and transferring signals; however, its fluidity can easily be affected by abiotic stresses. Compatible solutes


the present study, the higher expression level of *SS*, *SPS* and *HT* in transgenic plants was consistent with the elevated osmolyte concentration under salt conditions, resulting in improved ability to adjust in response to salt stress. The protein encoded by *ERD10C* is thought to protect macromolecules and membranes under stress conditions [@pone.0059924-Kasuga1], [@pone.0059924-Grover1], which is consistent with our finding of higher expression of *ERD10C* in transgenic than WT plants under salt stress. All these results suggested that ectopic expression of *UGT85A5* positively altered the expression of these stress-related genes, leading to enhanced tolerance to salt stress in transgenic lines. UGT85A5 has previously been identified as being induced by a combination of drought and heat stress [@pone.0059924-Rizhsky1]. We analyzed the *UGT85A5* promoter and found that it has many bindingQ:

How to obtain arbitrary distribution in quantum database

I was working on the Grover's algorithm and the most common example is for a unitary distribution in a quantum database, for example:
$|\psi\rangle = \frac{1}{2}|00\rangle + \frac{1}{2}|01\rangle + \frac{1}{2}|10\rangle + \frac{1}{2}|11\rangle.$
Is there a way to obtain arbitrary distribution (the above one is achieved by applying $H^{\otimes n}$ gates), e.g.
$|\psi\rangle = \frac{1}{3}|00\rangle + \frac{1}{4}|01\rangle + \sqrt{\frac{83}{144}}|10\rangle + \frac{1}{2}|11\rangle$
? Does the structure of Grover's algorithm differ in such a case?

A:

According to this paper,

A significant conclusion from this solution is that generically the generalized algorithm also has $O(\sqrt{N/r})$ running time

Where 'r' is the number of marked states. By generalized, the authors meant a distribution with arbitrary complex amplitudes. So it seems to answer your question. That the modified initialization would still perform in


$\pi^+\pi^-J/\psi$ decays are also identical for X(3872) and $\psi'(3686)$ \[2\]. These measurements provide additional support for our contention that the X(3872)$\to\pi^+\pi^-J/\psi$ events owe their origin to the vector $|c\bar{c}>$ content of X(3872).

The obvious shortcoming of our model for X(3872) is our lack of knowledge of the decay channels and the partial widths for the vector glueball component of the X(3872) wave function. The closest analogy we can make is to the three gluon annihilation widths of $J/\psi$ ($\approx$60 keV) and $\psi'$ ($\approx100$ keV). If the decay of a vector glueball with three gluons can be modeled after these decays of $J/\psi$ and $\psi'$, the contribution of the glueball component of X(3872) to its total width is likely to be at a sub-MeV level. Further, it would appear that094019. T. Barnes and S. Godfrey, *Phys. Rev.* [**D 69**]{} (2004) 054008. E. S. Swanson, *Phys. Lett.* **B 588** (2004) 189. N. A. Törnqvist, *Phys. Lett.* **B 599** (2004) 209. C. J. Morningstar and M. Peardon, *Phys. Rev.* [**D60**]{} (1999) 034509. F. E. Close and A. Kirk, *Phys. Lett.* **B 483**, 345 (2000);\
W. Lee and D. Weingarten, *Phys. Rev.* **D 61**, 014015 (1999). S. Dobbs et al., CLEO Collaboration, `hep-ex/0410038`.

[^1]: We note that CLEO \[16\] obtained the limit $f<20\%$, on the basis of entirely different assumptions about $B$ decays.


himself. It's loaded with funny content and all the best features of a social network. And the best part? Everyone with a subscription to The Bee gets full access at no extra cost. Get FREE Access *with premium subscription to The Babylon Bee– not even members of parliament – should meddle”.

Mr Macron has been supportive of the Spanish government on the Catalan issue. Former French prime minister Manuel Valls, who is Catalan, is running as a candidate for mayor of Barcelona with the backing of the stridently unionist Ciudadanos party.


In vitro-induced differentiation of bone marrow mesenchymal stem cells into melanocytes.
Large numbers of autogenous melanocytes (Mcs) are required when conducting studies on tissue engineering of skin and performing surgical treatment of depigmentation diseases. This study was conducted to explore the possibility of inducing differentiation of bone marrow mesenchymal stem cells (MSCs) into Mcs as a means of providing autogenous Mcs for purposes of tissue engineering and clinical treatment. MSCs were harvested from the bone marrows of black mice; and after six passages, hydrocortisone, insulin, transferrin and fibroblast growth factor were applied to induce their differentiation into Mcs. The morphological and ultrastructural characteristics of the newly differentiated cells were observed. The transcription and expression of multiple markers were examined using qRT-PCR, Western blot, and immunofluorescence analysis. Cell cycle phasesTag Archives: having fun

Parenting can be hard but being a single parent can be really, really hard. Not having someone to share the duties with is exhausting. There’s often not enough hours in the day to get everything you need to do done. And when you’re sick that gets even harder.

That’s been me this week. Fighting this brutal sinus/ chest cold thing that started with a painful sore throat and no voice. I work in radio, I need my voice. And I have to work. We’re short one announcer so sick days aren’t an option. Continue reading →


and bubble formation (as opposed to providing energy to accomplish a greater crystalline phase change) than in a grain kernel; (2) the pelletized rice has been pre-floured, hence the mechanical, and physical boundaries of the cellular structure have already been broken down leading to a more uniform expansion with less (heat) energy required to break down the cellular structure; and (3) the uniform pellet structure has a more uniform distribution of both starch and moisture for improved crisp puffing.
According to another aspect of the invention, the extruded food starch pellets may be cooled under sufficiently controlled (slowly) parameters to reduce stress in the pellet (i.e. an annealing of the pellets). This should reduce the energy required for possible annealing in the popping chamber. Also, the slow drying shouldabout 5:15 P.M. while observing "Ellis's home," now located at 366 Vane Street, Revere, the officers "saw one Ronald Rossotti with other known addicts in Rossotti's car ... pull up to Ellis's home and ... enter same." (6) On July 28, 1967, at approximately 4:30 P.M. the officers saw "one `Chuckie' Chiampi and Richie Pisani leave Ellis's home in Pisani's vehicle." That evening at about 8 P.M. the officers' informant told them that "Pisani and Chiampi had picked up a bundle of heroin that day and sold same in East Boston. As a result of further conversations with informants ... [the officers] learned that Pisani and Chiampi were the main pushers of heroin for Ellis in the East Boston area." (7) At approximately 8:15 P.M., on August 7,


isLoading: true });
    }
  }

  componentDidUpdate(prevProps) {
    console.log(this.props.user, prevProps.user);
    if (prevProps.user !== this.props.user) {
      const item = getItemByUserId(this.props.user.id);
      this.setState({
        value: { item }
      });
    }
  }

  render() {
    const { isLoading, value } = this.state;
    if (isLoading) {
      return
Loading data...
;
    }
    console.log(value);
    return <Provider value={value}>{this.props.children}</Provider>;
  }
}

export { ItemProvider, Consumer as ItemConsumer };

Working demo

CALL GOPEN (OGPWG,Z(IBUF),1)
      DO 42 I = 104,150
   42 Z(I) = 0
      Z(101) = 1
      Z(102) = 13
      Z(103) = IPOINT
      Z(110) = 78*2
      CALL WRITE (OGPWG,Z(101),50,0)


<?php

/*
 * This file is part of the Symfony package.
 *
 * (c) Fabien Potencier <fabien@symfony.com>
 *
 * For the full copyright and license information, please view the LICENSE
 * file that was distributed with this source code.
 */

namespace Symfony\Component\VarDumper\Tests\Caster;

use PHPUnit\Framework\TestCase;
use Symfony\Component\VarDumper\Caster\Caster;
use Symfony\Component\VarDumper\Test\VarDumperTestTrait;
use Symfony\Component\VarDumper\Tests\Fixtures\GeneratorDemo;
use Symfony\Component\VarDumper\Tests\Fixtures\NotLoadableClass;

/**
 * @author Nicolas Grekas <p@tchwork.com>
 */
class ReflectionCasterTest extends TestCase
{
    use VarDumperTestTrait;

    public function testReflectionCaster()
    {
        $var = new \ReflectionClass('ReflectionClass');

        $this->assertDumpMatchesFormat(
            <<<'EOTXT'
ReflectionClass {
  +name: "ReflectionClass"
%Aimplements: array:%d [
    0 => "Reflector"
%A]
  constants: array:3 [
    "IS_IMPLICIT_ABSTRACT" => 16
    "IS_EXPLICIT_ABSTRACT" => %d
Kimberly Phillips

Office Phone: (540) 432-4131

Email:

Main Campus, Harrisonburg

Office Location: n/a

Office Hours: 9:30am-4:00pm by Appointment

Director

Career Services

Prior to joining the staff at EMU in August 2014, Kimberly was Assistant Director of Career Development at Washington and Lee University in Lexington, Virginia. The scope of her work included advising students on majors, internships, job searches, professional documents, interviewing and graduate school. Kimberly managed the recruitment program and worked directly with employers hiring W&L students. She also completed a year-long career counseling internship program in Career Services at Georgia State University during her graduate studies.

Kimberly and her husband Matt reside in Lexington and they have one cat, Cookie.

Education

Master of Science in Professional Counseling, Georgia State University, 2008

Bachelor of Arts in Criminal Justice, Minnesota State University Moorhead, 2006


Q:

shortest distance of $x^2 + y^2 = 4$ and $3x + 4y = 12$

$x^2 + y^2 = 4$ and $3x + 4y = 12$
line from origin with gradient $\frac{4}{3}$ intersect circle at $(\frac{6}{5} , \frac{8}{5}) $.
intersect $3x + 4y = 12$ at $(\frac{36}{25}, \frac{36}{25})$.
so distance is $\sqrt{{(\frac{36}{25}- \frac{6}{5})}^2 + {(\frac{36}{25}- \frac{8}{5})}^2} = \frac{\sqrt{52}}{25}$
but my answer is wrong. Where am i wrong?

A:

No need to find the intersection, that is no need for quadratics.  The normal to $y=-\frac34x+3$ through the origin is given by the equation $y=\frac43x$; determine the intersection point of both to find that the distance from the line to the origin is $\frac{3}{\sqrt{1+\left(-\frac34\right)^2}}=\frac{12}{5}$.  Hence the distance from the circle to the line is $\frac{12}{5}-2$.
NB: In general the distance from $y=mx+b$ to the origin is $\frac{|b|}{\sqrt{1+m^2}}$

May 1781.

Career
Robe served from June 1782 to July 1784 at Jamaica, acting as adjutant and storekeeper. After two years at home he was in 1786 sent to Canada. He was promoted to first lieutenant on 22 November 1787, and returned to England in 1790.

In April 1793 Robe went to Holland with the artillery under Major Wright, part of an advanced force of the Duke of York's army for the Flanders Campaign, the main body of artillery under Sir William Congreve embarking in May. Robe took part in the siege defence operations at Willemstad, with which the English share of the campaign commenced. He was appointed, in addition to his ordinary duties, acting adjutant and quartermaster, and at the instance of Congreve, he was made inspector of ammunition.


that to $$ (\lnot p\to p)\to p,\lnot\lnot p, \lnot p\vdash p,$$ and we see we have a statement and its negation to the left of the turnstyle and we're done.
Observe that the left-hand side of the turnstyle in your title has the schematic form of proof by contradiction (note, nothing would change with the above proof if we replaced with $(\lnot p \to \bot)\to p$, and indeed this does not change the semantics of the statement, since we can prove $\bot$ from $\lnot p$ if and only if we can prove $p$ from $\lnot p$). Thus, we might expect this to be an equivalence. And it is... try to prove the other direction.

in determining this productivity, a pilot study was conducted, in which fifty latrines were observed in and around Ifakara, Tanzania. The characteristics of the latrine superstructure, use of the latrine, and chemical characteristics of pit latrine contents were compared to the numbers of flies collected in an exit trap placed over the drop hole in the latrine. Absence of a roof was found to have a significant positive association (t=3.17, p=0.003) with the total number of flies collected, and temporary superstructures, particularly as opposed to brick superstructures (z=4.26, p<0.001), and increased total solids in pit latrines (z=2.57, p=0.01) were significantly associated with increased numbers of blowflies leaving the latrine. The number of larvae per gram was significantly associated with the village from which samples were taken, with the


largest difference between two villages outside Ifakara (z=2.12, p=0.03). The effect of latrine superstructure (roof, walls) on fly production may indicate that improvements in latrine construction could result in decreases in fly populations in areas where they transmit diarrhoeal pathogens.

Funding: This research received financial support from the Bill and Melinda Gates foundation (grant number OPP52641) and the Sanitation and Hygiene Applied Research for Equity (SHARE) consortium funded by the UK Department for International Development (grant number P04990). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Introduction

Globally, diarrhoea is the leading cause of mortality among children under 5 [1]. Diarrhoeal disease has multiple transmission routes, and its controlHospital Consumer Assessment of Healthcare Providers and Systems (HCAHPS) scores and resulting adjustments in Centers for Medicare & Medicaid Services (CMS) Hospital Value-Based Purchasing (VBP) payments. We then performed sensitivity analyses to create a return on investment (ROI) lookup table for hospitals of varying size and staffing costs that are considering implementation of an inpatient VR program. Our objective with this hypothesis-generating analysis was to create a framework for future health economic analyses of inpatient VR therapy and to determine the cost and effectiveness thresholds at which point inpatient VR therapy for pain management becomes cost-saving.

Results {#Sec2}
=======

Base-case results {#Sec3}
-----------------

Implementing a VR therapy program in the inpatient setting provided an average of \$5.39 (95% confidence interval of --\$11.00 to \$156.17) in cost-savings per patient when compared to usual care.


cost-saving. For all sensitivity analyses, the model accounted for additional costs associated with purchasing more VR licenses and hiring additional staff as the number of patients using VR therapy increased (Supplementary Table [8](#MOESM1){ref-type="media"}).

We also conducted Monte Carlo probabilistic sensitivity analysis using 1000 simulations and assumed that all variables followed a triangular distribution with base-case, minimum, and maximum values listed in Table [3](#Tab3){ref-type="table"}.

Data availability {#Sec22}
-----------------

Data sharing is not applicable to this article as no datasets were generated or analyzed during the current study.

Electronic supplementary material
=================================

 {#Sec24}

Supplementary Tables(DOCX 30 kb)

**Publisher\'s note:** Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

**Change history**

8/23/2018

The author would like to update the competing interest statement, which in the original published Article stated "B.M.R.S. has received a research grant, administereda frequent visitor to Whirlpool, especially during the height of the debate over the NBN project which he had oversight and ministerial responsibility for. The Senator mentioned a number of specific Whirlpool threads on several occasions in Federal Parliament.

In addition, in August 2012 Conroy delivered a fiery tirade against the media for constantly repeating misconceptions about Labor’s National Broadband Network project, singling out the Financial Review newspaper for particular ridicule and recommending that those interested in accuracy read broadband forum Whirlpool.

“For those that are interested in a comprehensive discussion of these issues, I can recommend to you the Whirlpool website, particularly the thread entitled ‘fighting the FUD’,” Conroy said at the time. “It is a very informative thread, and I would encourage you to take a look at


Depends on which granite room you visiting.

But while there in Washington DC I also turned my mind to all of those who have tried to right the wrongs from this very city. I think of my hero Ron Paul. He believes, as do I, that the first thing that needs to be fixed is our monetary system starting with the federal reserve system scam. He’s well aware of the information included in the very well presented video below. But he still stands by his convictions. That’s the kind of person we need at the helm trying to bring this runaway train to a screeching halt.

I know one thing for sure. It takes a man far braver than I to chance the odds and track record of going againstbut for now we are only shrouded in questions.

On June 29, 1968, the Purdue Aviation Corporation charter flight was carrying 23 passengers from Kankakee, Illinois to a national Lion’s Club convention in Dallas, Texas. It was a clear summer day without a cloud in th sky. Somewhere over Rolla, Missouri, Jerrold I. Potter excused himself to use the restroom and proceeded to make his way to the rear tail section of the plane. . . . Read complete report

Isn’t Big Brother, Just Family?: Government Protects its Citizens, by Keeping Them in the Dark

by Paul Schroeder

Knowing full well that government disclosure of UFO/alien realities would cause complete religious and social societal disintegration, our government has thoughtfully and parentally organized covert agencies of disinformation to sustain the fabric of societal


Q:

Saving data into bootable pendrive

I've created a bootable USB stick for Ubuntu 13.04, and my windows and linux machines still recognize it as a normal FAT32 flash drive with data in it. Would it still work the linux boot if I saved more data in it as long as I don't delete anything that the USB boot installer put there?

A:

Yes, it could still function as a normal usb drive (be it a bootable one).
I would recommend putting your extra files in a directory but that's optional.
Please do keep in mind that if you've enabled your bootable usb to save changes to the usb that you should keep about 50 to 100 mb of space free from data.

per cent hold the opposing view. But in the end, public opinion could go either way as almost one third (28 per cent) said it’s “too soon to tell” and another seven per cent “didn’t know.”


package com.qcadoo.mes.materialFlowResources.batch;

import com.qcadoo.localization.api.TranslationService;
import com.qcadoo.mes.basic.controllers.BasicLookupController;
import com.qcadoo.mes.basic.GridResponse;
import com.qcadoo.mes.basic.LookupUtils;
import com.qcadoo.mes.materialFlowResources.DocumentPositionService;
import com.qcadoo.mes.materialFlowResources.ResourceDTO;
import com.qcadoo.mes.materialFlowResources.WarehouseMethodOfDisposalService;
import org.apache.commons.lang3.StringUtils;
import org.springframework.beans.factory.annotation.Autowired;
import org.springframework.context.i18n.LocaleContextHolder;
import org.springframework.http.MediaType;
import org.springframework.stereotype.Controller;
import org.springframework.web.bind.annotation.RequestMapping;
import org.springframework.web.bind.annotation.RequestMethod;
import org.springframework.web.bind.annotation.RequestParam;
import org.springframework.web.bind.annotation.ResponseBody;

import java.util.*;

@Controller
@RequestMapping(value = "resource")
public class ResourceLookupController extends BasicLookupController<ResourceDTO> {

    @Autowired
    private DocumentPositionService documentPositionService;

    @Autowired
    private WarehouseMethodOfDisposalService warehouseMethodOfDisposalService;

    @Autowired
    private LookupUtils lookupUtils;

    @Autowired
    private TranslationService translationService;

    @Override
    @ResponseBody
    @RequestMapping(value = "records", method = RequestMethod.GET, produces = MediaType.APPLICATION_JSON_VALUE)
    public GridResponse<ResourceDTO> getRecords(@RequestParam String sidx, @RequestParam String sord,
            @RequestParam(defaultValue = "1", required = false, value = "page") Integer page,
         PRODUCT DESCRIPTION

Modern Studio Doorway Dolly with 2 Wheel Steering

The Modern Studio Doorway Dolly with 2 Wheel Steering is still a popular tool for an inexpensive and quick dolly shot. The Doorway Dolly can also run on dolly track with optional track wheels for a super smooth move. Features two wheel steering and carpeted platform with camera tie down point. Includes 2 sideboards for extra width when needed and 2 push handles. Telescoping rear axle for greater stability when needed.


things done," LaBolt said.

Sanders is also facing lingering questions about his age, having suffered a heart attack late last year. He is the oldest candidate in the race, and, if elected, he would be the oldest president in U.S. history.

Former Colorado Sen. Gary Hart, who is supporting Bennet's underdog bid, was reluctant to single out any of the candidates for criticism. But he said Sanders wouldn't be the strongest nominee and suggested it was fair to take Sanders' age into account.

"I think health has become an issue, whether we like it or not," Hart, 83, said in an interview. "I’m older than Sen. Sanders, so I can say things like that. I think it’s time for generational change."

Marshall Matz, who was a policy adviser for Sen. George McGovern'sracing cars, two boats, two motorcycles and two all-terrain vehicles.

The arrests started about 8:30 p.m. yesterday when Lazaro Ramirez, the purported Florida connection for the cocaine, drove to the Baltimore area in a 1989 Toyota Camry. Originally, Marks said, Ramirez was scheduled to meet his Baltimore connections at a local motel but changed the meeting place to a hangar-like warehouse in Elkridge.

Four of the arrests took place there, he said.

Agents seized the Toyota immediately, Marks said, and searched it for narcotics. "We pretty much pulled it apart," he said. Police found the 13 pounds of cocaine -- nearly six kilograms -- in a false gasoline tank and in the vehicle's firewall between the engine and --board.

"Ramirez was going to switch the drugs for money" with another man, Marks


A configurational model for silicon oxide damaged after a high-dose ion implantation of a nonreactive species is presented. Based on statistics of silicon-centered tetrahedra, the model takes into account not only the closest environment of a given silicon atom, but also the second neighborhood, so it is specified whether the oxygen attached to one given silicon is bridging two tetrahedra or not. The frequencies and intensities of infrared vibrational bands have been calculated by averaging over the distributions and these results are in agreement with the ones obtained from infrared experimental spectra. Likewise, the chemical shifts obtained from x-ray photoelectron spectroscopy (XPS) analysis are similar to the reported values for the charge-transfer model of SiOx compounds.of deterioration in the sulfonate coating upon long storage before reheating and applying the asphalt at a later date. Whether the bonds are semi-ionic or covalent, it is readily apparent that the conversion to sulfonates of the exposed sites where metals are present upon the face of the aggregate is vastly superior to attempting to first formulate such compounds and then to try to discover a way to cause them to become bonded to the aggregate afterwards. This is, in effect, what the other compounds such as emuslifying agents and anti stripping agents sought to achieve.
In addition to the presence of sulfonate deposits upon the surface of the aggregate as an aid to the coating of asphalt, properly selected sulfonic acid sources serve another important function. When a


is treated and dried, he can introduce asphalt and solution simultaneously or the solution and then the asphalt prior to or concurrent with heating or he can, if necessary, introduce the solution subsequent to heating and applying the asphalt. While, in theory, the best results are to be expected when the solution and then the asphalt are applied prior to heating, excellent results have been achieved using the other sequences described.
It has previously been thought necessary that such a hot mixture of aggregate and asphalt must be kept dry and heated to approximately 300.degree. F. to assure proper bonding on location. It has been found that by adding a dilute solution of sulfonic acid and water to aggregate or aggregate plus asphalt, the asphalt percentage can be reducedthe Cutler machine.
Another object of this invention is to provide a means whereby standard roadbuilding and maintenance equipment supplemented by various means for supplying heat may be able to recycle all of the existing asphaltic surfacing in situ and thus dispense with the need for hauling it to a hot plant facility.
Another object of the present invention is to provide a means whereby standard equipment and supplemental sources of heat may be able to reuse all of the recycled asphalt as surfacing or, at the discretion of the engineer, apportion part of it for use as base material.


Renal scanning 99mTc diethylene-triamine pentaacetic acid glomerular filtration rate (GFR) determination compared with iothalamate clearance GFR in diabetics. The Collaborative Study Group for The study of Angiotensin-Converting Enzyme Inhibition in Diabetic Nephropathy.
Traditionally, creatinine clearance is used as an estimation of the glomerular filtration rate (GFR) because of its relative ease and low cost. Errors in collection limit its usefulness. Estimation of GFR using 99mTc diethylene-triamine pentaacetic acid (Tc-DTPA) by direct scintigraphic determination of fractional radionuclide accumulation within each kidney does not require blood or urine sampling, takes 10 to 15 minutes to perform, and has been reported to give a GFR that correlates with 24-hour urinary creatinine clearance (CC) in hospitalized patients (r = 0.95). To assess its usefulness in the outpatient diabetic with nephropathy, 24 patients withalso calculated the necessary sample sizes in studies in which the study drug is compared to a typical antipsychotic or placebo. The pooled efficacy rates observed were 40.8%, 34.9% and 21.3% for the atypical antipsychotics, typical antipsychotics and placebo, respectively. One hundred and sixty six patients would have to be included when a new drug is compared with placebo if calculation is based on a difference of 20% found between the atypical antipsychotic and placebo and 2,054 if the difference sought were that found between the atypical antipsychotic and the typical antipsychotic, i.e. 6%. The estimated therapeutic failures would be 115 of the 166 patients when the study drug is compared with placebo, and 1,274 failures in the 2,054 patients when the study drug is compared to the


VentureBeat » U.S. House News | VentureBeathttp://venturebeat.com
News About Tech, Money and InnovationTue, 31 Mar 2015 18:18:27 +0000en-UShourly1http://wordpress.org/?v=4.1.1Copyright 2015, VentureBeatNetflix to finally launch social sharing in early 2013 after Obama signs billhttp://venturebeat.com/2012/12/26/netflix-facebook-sharing-law/
http://venturebeat.com/2012/12/26/netflix-facebook-sharing-law/#commentsWed, 26 Dec 2012 20:35:03 +0000http://venturebeat.com/?p=595900Netflix will let subscribers in the United States connect their profiles to Facebook as soon as President Obama signs a popular bill into law, the company confirmed today.
]]>Gaming execs:Join 180 select leaders from King, Glu, Rovio, Unity, Facebook, and more to plan your path to global domination in 2015. GamesBeat Summit is invite-only -- apply here. Ticket prices increase on April 3rd!

Netflix will enable subscribers in the United States connect their profiles to Facebook as soon as President Barack Obama signs a popular bill into law, the company confirmed today.

The bill, which will\geq 0$.

*Proof of Theorem \[th.derivada\].* Differentiating [(\[def.NFstar\])]{}, for $m=n$, with respect to $t$ we obtain $$\begin{aligned}
  v'_{n+1}(t)  = &  \label{deri}
   \frac{1}{c_n^2(t)}v'_{n} (t)
 \Big(c_n(t) \int_0^{{| \O |}}{\mathcal{K}}_h'(v_{n}(t)-v_{n}(s)) v_{n}(s) ds \\
 & - \int_0^{{| \O |}} {\mathcal{K}}_h(v_{n}(t)-v_{n}(s))v_{n}(s)ds
   \int_0^{{| \O |}}{\mathcal{K}}_h'(v_{n}(t)-v_{n}(s)) ds \Big). \nonumber\end{aligned}$$ Let us consider the case $n=0$. By assumption, $v_0 \in W^{1,p}(0,{| \O |})$. On one hand, since ${\mathcal{K}}$ is continuous and positive in a neighborhood of $0$, and $v_0'$ is bounded in $L^1(0,{| \O |})$, we deduce $c_0(t) \geq \hat c \int_{{\mathbb{R}}} {\mathcal{K}}_h(\xi) d\xi$, for some positive constant $\hat c$.

On the other hand, the regularity assumed on $v_0$ also implies $v_0\in L^\infty(0,{| \O |})$. Using these properties together with the Lipschitz continuity of ${\mathcal{K}}$ we obtain that also $v_1\in


producing between eight iterations, for the Squares image, and twenty iterations, for the Texture image. We used the same parameter values for $h$ and $\rho$ in all the experiments.

As expected, the best visual result for the natural image is obtained with the Nonlocal Means filter: smoother and with a lower staircaising effect than the others. It is interesting to notice how the absence of local information in the Neighborhood filter produces regions with rapid intensity value changes, for instance in the clouds of the image. The smoothing effect of the local terms in the Bilateral and the NLM filters prevent the formation of this artifact.

A partial explanation of the worse behavior of the NF may be found in the corresponding plots for the histograms and the decreasing rearrangements.in VHL \[[@b3]\]. The VHL protein pVHL) has been implicated in a variety of functions, including transcriptional regulation, posttranscriptional gene expression, extracellular matrix assembly, protein folding and ubiquitination as reviewed by Kaelin \[[@b10]\].

An increasing number of germline mutations have been reported in VHL-affected individuals \[[@b11]\], and genotype-to-phenotype correlations are now emerging \[[@b15]\]. Somatic mutations in VHL have also been detected in several types of sporadic and hereditary tumours \[[@b16]\].

Phenotypes vary among families, reflecting genotypic differences \[[@b1]\]. Clinically, VHL is classified in type 1 or type 2 based on the absence or presence of pheochromocytomas. The occurrence of renal cell carcinoma (RCC) allows a further distinction between type 2 A (low risk of RCC) and type 2 B (high risk of RCC). Some type 2 families develop pheochromocytomas only,


with no other neoplastic findings of VHL (VHL-type 2 C) \[[@b20]\]. VHL tumours, including pheochromocytomas and paragangliomas, may appear clinically to be sporadic but represent milder cases of VHL, with the attenuated phenotype resulting from either a mild impairment in function of the mutated pVHL or somatic mosaicism. The latter is a condition in which genetically different cells coexist in tissues of the same individual, and the intratumoural mixture of VHL-mutated and VHL-non-mutated cells clearly can modulate the resulting phenotype.

We have analysed the VHL gene in the available members of a VHL family in which the pro-band presented with bilateral pheochromocytomas and multiple paragangliomas. Her father showed what we believe is a very mild and relatively late-onset VHL phenotype. In this study, we describe the somatic mosaicism offrom her parents, one brother and one sister was uninformative, except for the father\'s history. The pro-band\'s relatives underwent physical examination.

![MR image of the pro-band\'s brain.The arrow indicates a 1.6-cm-diameter right-sided supratentorial frontal meningioma.](jcmm0011-1408-f1){#fig01}

The patient\'s father, now 51 years old, was found to have an angioma of the glans penis and had had surgery for a mandibular cyst and epididymal cystadenomas at age 43 years. Hence, we suspected that the father had an attenuated form of VHL. Indeed, abdominal ultrasonography and total-body magnetic resonance imaging (MRI) revealed a 2.3-cm cyst in his right kidney. His blood pressure and levels of plasma free metanephrines, fractionated urinary metanephrines, chromogranin A were normal. The other three first-degree relatives were normal both clinically and biochemically.

Genetic investigations
----------------------

Informed consent for genetic testing was obtained


would allow the development of unified surveillance guidelines for VHL patients or those at risk for this disease.

In summary, we have provided molecular evidence of somatic mosaicism in the father of a patient with full VHL. Because of the incomplete penetrance of the disease the diagnosis of VHL was first not recognized. Because of penetrance age related in VHL, a long-term follow up is warranted. Counselling of patients and closely related family members must take a central place in the management of hereditary multi-organ cancer syndromes, such as VHL. A careful and complete clinical examination in the parents of each patient affected by an apparently *de novo* VHL germline mutation is warranted.

The evaluation of the parents of a pro-band with an apparent *de novo* VHL gene mutation should12*v**2 + 12*v - 4. Let y(b) = 4*h(b) + 3*w(b). Factor y(k).
4*k*(k - 3)*(k + 1)
Let h(r) = -r. Let g(f) = 4*f**2 - 3*f. Suppose -3*j = -3*y + 42, -3*y + j + 42 - 10 = 0. Let t = y + -4. Let p(s) = t*h(s) - g(s). Factor p(d).
-2*d*(2*d + 1)
Let o(m) be the first derivative of -2*m**6/3 + 12*m**5/5 - 2*m**4 - 8*m**3/3 + 6*m**2 - 4*m - 1. Solve o(a) = 0.
-1, 1
Let t(b) = -b + 1. Let z(k) = -k**2 - 2*k + 2. Suppose 0 = 3*g - 2*i - 15 + 1, -3*g - 4*i = 10. Let f be (-2 - 0) + g*2. Let l(n) = f*t(n) - z(n). Factor l(o).
o**2
Let t(d) = -d


the protein ensemble by (i) averaging the hydrogen bonding strengths from the entire ensemble and (ii) by refining the mathematical model of hydrogen bonds. Furthermore, we combine our FIRST-ensemble rigidity predictions with the ensemble solvent accessibility data of the backbone amides and propose a novel computational method which uses both rigidity and solvent accessibility for predicting hydrogen-deuterium exchange (HDX). To validate our predictions, we report a novel site specific HDX experiment which characterizes the native structural ensemble of Acylphosphatase from hyperthermophile Sulfolobus solfataricus (Sso AcP). The sub-structural conformational dynamics that is observed by HDX data, is closely matched with the FIRST-ensemble rigidity predictions, which could not be attained using the traditional single 'snapshot' rigidity analysis. Moreover, the computational predictions of regions that are protected from HDX and thosewith individual controls

Two tables with shared controls

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                      : @"NO";
    DLog(@"NSDeallocateZombies = %@", NSDeallocateZombies );
    NSString *NSEnableAutoreleasePool = (getenv("NSEnableAutoreleasePool"))
                                    ? [NSString stringWithCString:getenv("NSEnableAutoreleasePool") encoding:NSASCIIStringEncoding]
                                    : @"YES";
    DLog(@"NSEnableAutoreleasePool = %@", NSEnableAutoreleasePool );
   of biomedical products in Wuhan Chang Hong (Wuhan, China). Soybean phosphatidylcholine (SPC) and cholesterol (CH) were both obtained from Chengdu Kelon Chemical Reagent Works (Chengdu, China). Methanol and acetonitrile for HPLC analysis were of chromatographic grade and come from Shanghai Ludu Chemical Reagent Works (Shanghai, China). All the other reagents were of analytical grade and used as received. Healthy rabbits, weighing approximately (2.0 ± 0.5) kg, were supplied by the Experimental Center of Sichuan Agriculture University (Ya,an, Sichuan, China). The rabbits were thoroughly examined before experimentation and were kept for 7 days to ensure their clinical conditions. They were fed with fresh green fodder thrice daily and water was provided ad libitum. The study was carried out according to the principles of Institutional Ethical committee for animal experiments.

*Preparation


overcome the limitation of quickly absorb and easy to eliminate of Conventional preparations of CS. In the groups of CSLS, the values of *t*~1/2β~(p \< 0.05) and MRT(p \< 0.01) markedly increased by about 1.89-fold and 2.21-fold, respectively, in comparison to that of the solution group. In addition, CLs and K~10~ markedly decreased. All these Parameters demonstrated that CSLS gave a markedly larger MRT and longer residence time in the systemic circulation than CS solution group, exhibiting an obvious sustained--release effect. Meanwhile, in the groups of CSLS, the values of AUC markedly increased by about 2.79-fold (p \< 0.01), in comparison to that of the solution group. This show the bioavailability of CSLS is obviously higher than the Solution group. Therefore, it can be concluded that liposomal CSyou just made a fewsome days agoin the past? Any surepositivecertain?

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face of the user, opening up a range of useful possibilities from per-pixel focus to foveated rendering.

The patent appears to have been filed as far back as October, 2012, which offers further evidence to Sony’s claim that they’ve been working on VR for quite some time.watching something like Newsroom. You have to catch the words in the narrative and in the dialogue. Otherwise, you’re missing the point.

MY LATEST VIDEOS

MY LATEST VIDEOS

Granted, it’s not for everyone. While entertainment is still its primary objective, Parts Unknown can get deep and quite dark at times. One has to have a certain amount of interest in cultural diversity and a mind that’s open enough to accept cultural diversity, without turning judgmental, to appreciate it.

That’s what the show is about, essentially — showing people that there are ways of life we can’t even begin to imagine. Whether it’s the poverty or the excess, Bourdain tells the story. He goes to the poorest regions of Myanmar and Colombia, and enjoy the humble food cooked from the produce of the


no avail. Now they silently retreat. The Iranian agency FARS, though not always reliable, reported yesterday that all Turkish agents in Aleppo province in Syria were called back to Turkey. During the coup event something curious happened to one important person:

The top counter-terrorism official responsible for Turkey’s campaign against Islamic State did go to a “meeting” at the presidential palace in Ankara. He was later found with his hands tied behind his back, shot in the neck, according to a senior official.

Someone used the coup trouble to off the top Turkish ISIS contact. How very convenient. This is the only reported casualty at the presidential palace I have heard of. Who might have had an interest in removing this witness of Turkish relations with ISIS? Could this belittle dots point to a new direction in Turkish foreign policies. This is one of the Three western worries about Turkey namely "Turkey’s ideological drift away from the West."

The former Turkish colonel Hasan Atilla Ugur, in talk with an Iranian news site, claimed that the coup was launched by the CIA directed Gülenists to prevent such change in Turkish foreign policy and the move towards Iran and Russia. (The preacher Fetullah Gülen, accused of orchestrating the coup, is known to be extremely anti-Iran.)

A trusted mouthpiece of Erdoagn, the somewhat lunatic chief editor of YeniSafak, İbrahim Karagül, accuses the U.S. of attempting to murder Erdogan:

I am saying it loud and clear: The U.S. administration directly planned to kill the president of the Republic of Turkey and implemented this plan.


naive application of the maximum entropy principle, we begin with a simple classical example.\
[**Application of the maximum entropy principle to classical statistical mechanics – derivation of Boltzmann statistics:**]{} Consider a canonical ensemble of $N$ non-interacting particles that can occupy discrete, non-degenerate energy levels (the extension to the degenerate case is straightforward but results in more cumbersome equations) having an energy $\epsilon_j$, with $j=1,2,3,
....$. We impose an energy constraint: $Q_1 = \sum_j \langle n_j
\rangle \epsilon_j = E$, where $n_j$ is the number of particles in level $j$ and $\langle\ \cdot\ \rangle$ denotes the average value. The temperature is a measure of the average total energy. The goal is to determine the probability, $P$, of observing a given distribution of particles among the levels subject to a constraint on thecelebrated Bose-Einstein distribution: $$\label{BE}
P_{BE}(\mathbf{n}) \propto   e^{-\beta \sum_i n_i \ \epsilon_i}
\qquad n_j = 0,1,2,\ldots  .$$ On constraining $n_j=0,1$ for all $j$ one gets the Fermi-Dirac statistics. This result is pleasing because the $\mathbf{n}$ representation is in fact the appropriate one for deriving quantum statistics. The particles are indistinguishable and all the information that one has is encapsulated by $P(\mathbf{n})$. The conundrum is that the results obtained by applying the maximum entropy principle to $P(\mathbf{i})$ and then coarse-graining the result to obtain $P(\mathbf{n})$ is different from applying the maximum entropy principle directly to $P(\mathbf{n})$. In other words, the operations of maximizing the entropy and of coarse-graining do [*not*]{} commute.\
[**Relative entropy and resolution of the puzzle:**]{} We suggest that the correct and consistent application of the maximum entropy


| CCR_MEN | CCR_MSTA);
		/* Dummy read */
		readb(i2c->base + MPC_I2C_DR);
	}

	for (i = 0; i < length; i++) {
		u8 byte;

		result = i2c_wait(i2c, timeout, 0);
		if (result < 0)
			return result;

		/*
		 * For block reads, we have to know the total length (1st byte)
		 * before we can determine if we are done.
		 */
		if (i || !recv_len) {
			/* Generate txack on next to last byte */
			if (i == length - 2)
				writeccr(i2c, CCR_MIEN | CCR_MEN | CCR_MSTA
					 | CCR_TXAK);
			/* Do not generate stop on last byte */
			if (i == length - 1)
				writeccr(i2c, CCR_MIEN | CCR_MEN | CCR_MSTA
					 | CCR_MTX);
		}

		byte = readb(i2c->base + MPC_I2C_DR);

		/*
		 * Adjust length if first received byte is length.
		 * The length is 1 length byte plus actually data length
		 */
		if (i == 0 && recv_len) {
			if (byte == 0 ||amount of at least 20 wt. %, relative to the total weight of the thermally conductive plastic material.
U.S. Published Patent Application No. 2011/0281051 in the name of Dufaure et al. discloses an expanded graphite, in which the specific surface is between 15 and 30 m2/g, the apparent density is less than 0.1 g/cm3, for an average particle size of more than 15 μm, to grant a thermoplastic polymer properties of thermal, electric and rheological conductivity suitable for the transformation of said polymer.
Takeuchi et al., in PCT Published Patent Application No. WO 2011/013645, describe a polycarbonate resin composition which contains, per 100 parts by mass of (A) a polycarbonate resin, 30-100 parts by mass of (B) artificial graphite, 0.01-5 parts by mass of (C) an organopolysiloxane that has a


group selected from among a phenyl group, a methoxy group and a vinyl group, and 0.01-5 parts by mass of (D) a fluorine compound. Also disclosed are: a molded body which is obtained by molding the polycarbonate resin composition; and a component for an electrical/electronic device, a case for an electrical/electronic device and a chassis for an electrical/electronic device, each comprising the molded body. The polycarbonate resin composition provides a molded article which is said to have high thermal conductivity and high mechanical strength, while exhibiting high flame retardancy even in cases when the molded article is formed thin.
U.S. Pat. No. 8,221,885, issued to Miller, describes an injection moldable, thermally conductive polymer composition said to have ultra low CTE properties and suitable both for substrate applications in highTalks - Uplifting Those Who Don't Want to Be Uplifted

Uplifting Those Who Don't Want to Be Uplifted

Uplifting Those Who Don't Want to Be Uplifted

Satguru Bodhinatha Veylanswami
, 2003-03-09

Many of our members take care of elderly parents, which can be a stressful situation if the parent is in a negative state of mind and refuses to budge from that state. Bodhinatha frequently receives requests for how to positively manage this type of situation. He suggests that we cannot expect the person to be uplifted, it is enough to do the kindly act, to fulfill our duty in a loving way.

Unedited Transcript:

A related point came to mind this morning. We have a situation where many of our members are taking care of elderly parents, one way or another. Either the parents


it should not ruin the mood of everyone else in the family. They should not feel they are not doing their best.

This applies to everything we do for others in life. Take this same principle. Sometimes parents get discouraged because they do the best they can with their children and the children don't respond in a way that seems at all reasonable to what they are doing to try and help. So the parents don't feel good. "Gee, the child didn't respond to all my good advise, all my love and attention. They are still doing the same old, stupid things." The parent feels sad. But why should you feel sad? You have done your duty, you have done the best you can, you have earned good karmasome time ago. She was supportive as possible during his difficult time."

Just awful.

But we certainly think there is more than this side to the story.

Obviously, we sympathize with what he endured, but Russell is a grown man, and even if Katie treated him as poorly as he claims, we hope he takes accountability for his own actions in the relationship. Nobody forced him to stay with her as long as he did and certainly nobody forced him to attempt to take his own life.

But abusive relationships, even if they're verbal, can be tricky and difficult to get out of in their own right, so who knows what really went on?

We're just thankful that nobody was seriously hurt and that these two are not together anymore!

What do U think??


 UNITED STATES V. MONTOYA-GAXIOLA

                           SUMMARY**

                           Criminal Law

     The panel reversed a conviction for possessing an
unregistered sawed-off shotgun with a barrel of less than
eighteen inches in length, in violation of the National
Firearms Act, 28 U.S.C. § 5861(d), and remanded for a new
trial.

    The panel held that the district court erred by failing to
instruct the jury as to the mens rea element of the charge.
The panel explained that Ninth Circuitto prove that Gergen knew that the
shotgun was illegal, and the Government objected. Based on
that exchange and a specific request for clarification by the
jury during deliberations, the district court gave a
supplemental instruction stating that the “Government [was]
not required to show that the defendant specifically knew:
(1) the barrel length of the shotgun was less than 18 inches,
or (2) the overall length of the shotgun was less than 26
inches.” Id.

    This court applied Staples and concluded that the district
court had “erroneously eliminated the mens rea
requirement[,]” which was “an essential element of a
§ 5861(d) violation.” Id. at 724. In doing so, we adopted the
majority rule that Staples requires knowledge of the specific
characteristic of the sawed-off shotgun that brings it within
the Act, not simply knowledge that the shotgun is