were “inadvertently
included in the [2014 Consent] Order and should not have been made a part of the legal
description recited in the Order.”3 Consequently, this mistake in transferring the Vacant Lots is
both common and mutual, as the record reflects that the parties only intended to transfer
ownership of the Waterfront Property and Home Parcel.

3
  Defendants, relying on MCL 565.451d, argue that the trial court erred by considering WFB’s
Affidavit of Interest because such an affidavit cannot alter substantive rights of a party unless
signed by that party. Defendants miss that that statutory provision applies to affidavits recorded
in the office of a register of deeds for the purpose of correcting scrivener errors or omissions.
WFB’s Affidavit of Interest was not filed in a register of deeds for this purpose and, therefore,
MCL 545.451d isHuman hypertension represents a disease of multiple etiologies. Included among these is a sodium and volume dependent low renin form of hypertension. Drugs that act to control one aspect of hypertension will not necessarily be effective in controlling another.
Enkephalin, a natural opiate receptor agonist, is known to produce analgesia, and it is also known that enkephalin is inactivated by a neutral metalopeptidase (EC 3.4.24.11) frequency referred to as enkephalinase or enkephalinase A. Various NMEP inhibitors have been discovered which, by their inhibition of this enzyme elicit an analgesic effect. To date, no significant cardiovascular activity has been attributed to NMEP inhibitors. The NMEP inhibitor thiorphan was found to inhibit peripheral ACE, but to have little direct cardiovascular activity in its own right: blood pressure and heart rate were


not affected. See T. Baum, et al, "Enkephalinase A Inhibition by Thiorphan: Central and Peripheral Cardiovascular Effects", Eur. J. Pharm., 94(1983), pg 85-91.
One class of drugs which is known to be effective in treating some types of hypertension is angiotensin converting enzyme (ACE) inhibitors, which compounds are useful in blocking the rise in blood pressure caused by increases in vascular resistance and fluid volume due to the formation of angiotensin II from angiotensin I. For a review of ACE inhibitors, see M. Wyvratt and A. Patchett, "Recent Developments in the Design of Angiotensin Converting Enzyme Inhibitors" in Med. Res. Rev. Vol. 5, No. 4 (1985) pp. 483-531, incorporated herein by reference.
It has recently been discovered that the heart secretes a series of peptide hormones called atrial natriuretic factorsfeel better security and CCTV cameras, more door staff and plastic glasses are ways of preventing it."

She also feels bar staff and doormen working in licensed premises should also be told by their bosses to be stricter with drinkers visiting their premises.

"They shouldn't serve people who look drunk and they should be asked to leave," said Nadia.

She also feels the move towards introducing 24-hour drinking is also a bad idea.

"It will encourage people to stay in the pubs and drink until they drop," said Nadia.

And he claimed those serving inebriated customers should show more responsibility - and refuse more booze.

The Home Office report shows the rate of violent crimes linked to drunkenness went from 6.69 for every 1,000 Teessiders up to 8.95 - a leap of 33.8pc. The


1. Field of the Invention:
A one piece tube and microscope slide manipulative laboratory device for use in clinical procedures, and particularly the labor intensive clinical procedure of urinalysis. Urinalysis technique minimally requires that a urine specimen be first tested for specific gravity, which is an important indicator of kidney function and the regulation of electrolyte concentration in patients receiving intravenous fluid therapies. Secondly, urinalysis requires the analysis of any sediment which may be present in the urine, since sediment is an indication of various pathologies in the patient, and the urine sediment is necessarily prepared in a slide for placement under a microscope objective. The present invention, therefore, is a one piece tube and microscope slide manipulative laboratory device which is a one tube system for both measuringsuch, they simply illustrate known techniques for insuring a frangible connection in a laboratory device.
The patent to Smith illustrates a plastic filter assembly, which allows for certain urine analysis procedures without the necessity for centrifuging. As such, it is noted primarily to illustrate yet another approach to a disposable urinalysis device. Similarly, the patents to Clark, Speck, and Yee illustrate urinalysis techniques, and particularly techniques wherein a urine sample is ultimately evaluated by an optical density determination. These patents are noted merely to illustrate particular chemical reagent mixing techniques in the art of urinalysis, wherein conventional test tubes are used as the specimen container.
In summary, no prior patent is known which teaches a one-tube assembly specifically adapted for urinalysis, and one characterized by a test tube type reservoir


formulating such a policy. The army chief has said that the security of our people is foremost, which is appropriate. Given that it is unusual for a person in such a position to make such a statement, and given the power structure in the country, it seems that a change in policy is inevitable.

Pakistan has the option of walking out of this alliance on terror and may consider announcing that it is no longer part of the US-led war in Afghanistan — that it will fight terrorism within its boundaries alone. It would also be appropriate to state that the tribes have the right to defend themselves. This would be significant because it would trigger a lot of other dynamics. That may reduce, to a large extent, actsof its own downfall by following inconsistent strategies and enforcing them through ruthless and arrogant force. It is not for Pakistan to correct the course the US is taking or to wage a war along with it. We should endeavour to maintain good state-to-state relations. Yet it remains our prerogative to chart our own course as a nation and as a state. Pakistan is caught in the eye of storm; yet there is room for hope. Nations go through turbulent times. It is the leadership that steers it to safe shores.

The writer is a retired brigadier and formerly secretary Fata and home secretary Khyber Pakhtunkhwa.


I need to disable/grey out certain menu choices depending on security and the applicate state. I am using the zcedMenuCmd function and setting the GrayVar to "" or "~". There does not seem to be any changes in the menu.

I have tried prefixing with the group name, tear off name or just the GrayVar itself. Any ideas?premised on the assumption that all countries are built around the Rhine-Westphalian metal manufacturing firm, where the classical model of competitiveness does apply. Ireland’s manufacturing sectors have been in decline for over a decade (computer hardware, optical equipment and medical machinery). It is true that if these firms (and the government) had radically cut wage costs in the 2000s (in order to compete with Eastern and Central Europe) then these firms could have been kept in Ireland. But would we call this competitive success?

Figure 2: Trade in services as a percentage of GDP in selected countries

Source: World Bank

The most important observation on Ireland’s export-driven recovery since 2009 is that it has occurred in the internationally traded services sectors of the economy (see Figure 2). In particular, it is


base.

Along with Gosselink and McCracken, Will Miller rounds out the three-person firm. Miller was formerly at Zata|3. Control Point will take on political consulting, communications and issue advocacy projects. The company's services will include phone, direct mail and broadcast, as well as digital offerings—mobile video, social media and web design.

The firm officialy launched January 1, but already has a few clients on board. Says McCracken: "We're new, but we're hungry."Previous studies have shown that bilateral decompression has better clinical outcome scores than unilateral decompression.^[@bibr11-0300060519889458],[@bibr12-0300060519889458]^ Full decompression is the primary condition for eliminating clinical symptoms. Ample release is the premise of intervertebral space distraction and a guarantee of a reduction in spondylolisthesis. A long pedicle screw is favorable in the vertebral slip reduction process.^[@bibr13-0300060519889458],[@bibr14-0300060519889458]^ Although MIS-TLIF requires a relatively long-term learning curve,^[@bibr15-0300060519889458],[@bibr16-0300060519889458]^ this operation has many clinical advantages for treating lumbar spondylolisthesis. Many studies have shown that technical proficiency in MIS-TLIF is achieved after 44 surgeries, and patients benefit from a shorter operative duration and radiation, less pain, and more relief in their back, legs, and neurogenic symptoms.^[@bibr17-0300060519889458]^ The MIS-TLIF approach is a technically difficult procedure for practicing spinal surgeons regarding intra- and perioperative parameters of surgical time,


typical, modern computer (i.e., anything using twos complement), the conversion to unsigned is really a nop -- just a change in how the same bits are viewed.
Note that in a typical case, you can pre-compute upper-lower outside a (presumed) loop, so that doesn't normally contribute any significant time. Along with reducing the number of branch instructions, this also (generally) improves branch prediction. In this case, the same branch is taken whether the number is below the bottom end or above the top end of the range.
As to how this works, the basic idea is pretty simple: a negative number, when viewed as an unsigned number, will be larger than anything that started out as a positive number.
In practice this method translates number and the interval to theprecursor is not a determinant factor and suggests differences in enzyme activity such as lipoxygenase or inherent factors to the grain (higher antioxidant content, such as phenolics) that impact the formation of aldehydes through modulation of lipid oxidation pathways.

Seven esters, alcohols and ketones associated with floral, green and fruity notes were also identified ([Table 1](#molecules-24-02484-t001){ref-type="table"}) and are thought to arise from fermentation pathways. The concentration of five compounds was slightly impacted and present at 1.5- to 2-fold higher amount in the wheat versus the IWG sample. Typically, a higher amount of yeast would promote the formation of these compounds \[[@B11-molecules-24-02484]\]. However, in the current study, the two bread samples were formulated with the same amount of yeast, indicating that other parameters inherent to the grain are modifying the


consistency of the data and good agreement among the panel towards the sensory concept. Aroma compound concentrations were compared between wheat and IWG samples using t-test. All analyses were carried out using JMP Pro 13.1.0 (SAS Institute Inc., Carry, NC, USA).

**Sample Availability:** Samples of the compounds not are available from the authors.

Conceptualization, D.G.P.; Methodology, D.G.P.; Validation, D.G.P.; Formal Analysis, D.G.P. and L.P.; Investigation, K.A.S.; Resources, D.G.P.; Data Curation, D.G.P.; Writing--Original Draft Preparation, L.P.; Writing--Review & Editing, D.G.P., L.P., K.A.S.; Visualization, D.G.P.; Supervision, D.G.P.; Project Administration, D.G.P.; Funding Acquisition, D.G.P.

The authors would like to acknowledge the financial support provided by The Land Institute, Salina, Kansas, United States and the Flavor Research and Education Center at The Ohio State University and its supporting members.

The authors declare no conflict of interest.

![Aromalicense.

<http://aspnetwebstack.codeplex.com/>

Less so with MSSQL. But it's less of an issue there, because MSSQL provides a
very good view of what's going on under the hood to begin with, and Microsoft
has done an extremely good job of documenting the whole thing.

~~~
Locke1689
Having worked on the MSSQL source code -- even if you could see it it would
not help. It's a incredibly complicated monolith with a lot of historical
baggage. Not kind to fresh eyes. Remember that SQL Server was originally
Sybase SQL Server.

~~~
quadrant
That apocryphal last comment is irrelevant though as the code bases entire
split many, many, versions ago. (pre 7.0)

~~~
Tyrannosaurs
And if memory serves it's been through at least one, possibly two ground up
rewrites since then.

~~~
endersshadow
It's been through massive rewrites, but they didn't throw away the code and
start from scratch. They took five years


contracts in
the code gives you.

------
nickm12
This post seems to take the attitude that "documentation will always suck, so
just go right to the source". I think this attitude can impede software
projects with changing code (that is, all of them).

The problem is that the source can only tell you what a program does, not what
it is _supposed_ to do. If you don't know what it is supposed to do, it can be
difficult for consumers of the code to know whether some behavior is is
intended or a side-effect of the current implementation. Likewise, code
maintainers can be prevented from changing the implementation when they don't
know if consumers are relying on undocumented behavior. It's more difficult to
file bugs against undocumented code; how do you know it's a bug if you don't
know what the codeis supposed to do?

In brief, good documentation and good code are a virtuous cycle. Reading the
source is often necessary but it should be viewed as a failure of
documentation.

------
groby_b
Sigh. This is a rather myopic view of documentation.

Of course the source code is the ultimate arbiter of truth. But having a few
roadmaps to that source code is _incredibly_ valuable. And as long as the
underlying code does "what it says on the box", there's no reason to read the
code.

Reading your stack's source should be a last recourse, not the default mode of
operation. (Yes, I do read source code of my stack. Plenty of it. Which is why
I appreciate any occasion where I don't have to.)

And when I see his "brilliant HN post" mention that suggests that "sometimes,
you recompile your compiler", I'd


for.

If you do find yourself in the source code, being willing to play around with
it is invaluable for working out what's going on. If nothing else, you can
insert a printf to confirm that you're looking in the right place.

------
RyanMcGreal
> That's why, when it comes to code, all the documentation probably sucks. And
> because writing for people is way harder than writing for machines, the
> documentation will continue to suck for the forseeable future.

I don't think it sucks because it's harder to write. I think it sucks because
it's not strictly necessary to document your code in order to compile/ship it,
and it's easy to justify putting it off. Poorly documented code is a form of
technical debt, a compromise between getting it done _right_ and getting it
done _right now_.

------
drothlis
Some environments makelong time here. That might be a bit shallow of me….

Yamada: When I was looking through leaflets and job opportunities at my university’s entrance, I saw it and it felt right. What was good is that everyone’s real personality is gentle and honest. This is a company that treats people’s hearts as most important.

Question 3: KyoAni’s production quality is quite high; how long does the production time take? Also, please tell us times where you were glad you did something, failure stories, or points where you were working on each other’s works and thought it was amazing.

Ishihara: Our production timeframe isn’t different from others.

Takemoto: There’s not a big difference from when we start production from other companies. I don’t know about how they handle the schedule from there


considered golf?" "Might be easier on your back." "I don't like interruptions, Charlotte." "You know that." "I don't like hiking through the park in civvies, but there's a delicate matter to discuss." "Theresa Cullen has died." "Slipped down a crevasse attempting to secure our information." "Ruled an accident." "There are no accidents, not in here." "With all due respect, not everything is a part of this game." "Then you don't see the whole game." "Or perhaps you can no longer see beyond it." "Ford's stories are engaging." "For some, downright addictive." "But for all of Ford's obsessing with the hosts' verbal tics and convoluted backstories, most of the guests just want a warm body to shoot or to fuck." "They would be perfectly happy with something a little(GRADE) tools \[[@CR3]\]. Similar to our group's previous publications (e.g., see \[[@CR4]\]), quality of evidence was assessed as high to very low. Recommendations were stated as strong or weak on the basis additionally of indirectness of evidence, magnitude of effects, and availability, feasibility, and safety in resource-limited ICUs. We consulted the World Health Organization Essential Medicines List when considering availability of medications (available at <http://www.who.int/medicines/publications/essentialmedicines/en/>). When necessary, evidence from resource-rich ICUs was adopted after pragmatic experience-based appraisal (see online supplement). We also made several good practice statements \[[@CR5]\].

Results and recommendations {#Sec2}
===========================

The literature search for additional evidence from resource-limited ICUs identified several guidelines \[[@CR6]--[@CR8]\]; the only randomized trials were of metformin for the treatment of hyperglycemia \[[@CR9]--[@CR11]\]. Key recommendations are provided in Table [1](#Tab1){ref-type="table"}. Considerations informing each recommendation are described


/*
 * Copyright (c) 2016, Harald Kuhr
 * Copyright (c) 2016, Herman Kroll
 * All rights reserved.
 *
 * Redistribution and use in source and binary forms, with or without
 * modification, are permitted provided that the following conditions are met:
 *
 * * Redistributions of source code must retain the above copyright notice, this
 *   list of conditions and the following disclaimer.
 *
 * * Redistributions in binary form must reproduce the above copyright notice,
 *   this list of conditions and the following disclaimer in the documentation
 *   and/or other materials provided with the distribution.
 *
 * * Neither the name of the copyright holder nor the names of its
 *   contributors may be used to endorse or promote       int width = decoder.getDimX();
        int height = decoder.getDimY();

        // Single component, assumed to be Gray
        if (decoder.getNumComponents() == 1) {
            switch (decoder.getPrecision()) {
                case 8:
                    return to8Bit1ComponentGrayScale(decoded, width, height);
                case 10:


Immediate enteral feeding after abdominal surgery.
Enteral feeding by nasoenteral tube was begun immediately after surgery of the alimentary tract in 120 patients. In 37 cases the feeding was delivered proximally to a fresh anastomosis or enterotomy. Immediate enteral feeding was well tolerated even after major elective surgery of the gastrointestinal tract. The feeding promoted recovery of bowel function and evoked no major complications. Abdominal discomfort and diarrhea were the most common side effects. The response was less favorable after nonelective surgery associated with intraabdominal or systemic sepsis.researchers concluded that green tea polyphenols are effective in averting many of the detrimental effects of sunlight, and may thus serve as natural photoprotective agents.27 The photoprotective benefits of topical green tea have also been reported in other studies as well.28-30

A study from Korea found that when EGCG is topically applied to aged human skin, it stimulates the proliferation of epidermal keratinocytes, which increased the thickness of the epidermis.26 EGCG also inhibited the death of the keratinocytes after a period of exposure to ultraviolet radiation, thus conferring important photoprotection.

When topically applied or consumed orally, green tea polyphenols enhance cellsâ€™ protective responses to inflammation and various chemicals that promote tumor growth. At the same time, these phytochemicals prevent ultraviolet B-induced oxidative stress and immune system suppression.31

As people grow older,


during data collection and digitized continuously at 250 Hz with a 16-bit analog to digital converter. According to manufacturer's instruction, impedances were kept below 50kΩ. Offline, data were digitally high-pass filtered with a first-order 0.1 Hz filter, and digitally low pass filtered at 30 Hz (12 db/octave butterworth filter) in NetStation (version 4.5.7). For the ERN and Pe, data were then segmented from 400 ms prior- to 600 ms post-response for correct and incorrect trials. For the RewP, data were segmented from 200 ms before feedback presentation to 800 ms after feedback. Eye movements and blink artifacts were then corrected using independent components analysis (ICA) in the ERP PCA Toolkit in MatLab \[[@pone.0219883.ref062]\]. If any ICA component correlated with two blink templates (one template being provided by thedata were averaged across four fronto-central electrodes (6 (FCz), 7, 106, 129 (Cz); see \[[@pone.0219883.ref066]\] for electrode montage), as decided *a priori*. Electrode locations were chosen due to previous research suggesting that the ERN and RewP are maximal at these frontocentral locations (e.g., \[[@pone.0219883.ref067]\]). For the Pe, data were averaged across electrodes 54, 55, 61, 62, 78, and 79, as also decided *a priori*. All ERP component mean amplitudes for all trial types are reported in [Table 1](#pone.0219883.t001){ref-type="table"}.

10.1371/journal.pone.0219883.t001

###### Means and standard deviations for ERP components, task accuracy, and response time.

![](pone.0219883.t001){#pone.0219883.t001g}

  -----------------------------------------------------------------------------


match accordingly. Please see our Supporting Information guidelines for more information: <http://journals.plos.org/plosone/s/supporting-information>.

Additional Editor Comments (if provided):

Dear Dr. Muir,

I am very sorry for having delayed the decision on your manuscript, but there were circumstances that did not allow me to decide before.

Kind regards,

Thalía

\[Note: HTML markup is below. Please do not edit.\]

Reviewers\' comments:

Reviewer\'s Responses to Questions

**Comments to the Author**

1\. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer \#1: Yes

Reviewer \#2: Yes

\*\*\*\*\*\*\*\*\*\*

2\. Has the statistical analysis been performed appropriately and rigorously?

Reviewer \#1: Yes

Reviewer \#2: Yes

\*\*\*\*\*\*\*\*\*\*

3\. Have thewould individuals with more internal locus of control exhibit enhanced ERN and reduced RewP? Given that they do not attribute high control over themselves and give most of the credit to the environment, one would expect for them to show enhanced sensitivity to external feedback, and hence larger RewP amplitudes. Please comment.

5\. I don't get why the authors suggest that heightened Pe would be present in participants with increased perfectionism AND those with more internal locus of control. From the Introduction, one would think that perfectionists do not trust their own abilities to influence environment, so then enhanced Pe would be expected in perfectionist participants with low internal locus.

METHODS

6\. The authors decided to use only differential waves for their analyses, and they give an argument about that. Certainly,


48 in the Levenson scale) could be considered as a risk factor.

8\. Were ERN and RewP correlated across participants?

DISCUSSION

Please propose some clinical populations in which it would be relevant to explore the association between these personality traits and ERP.

MINOR

Line 259, page 12, "cents" instead of "centers"

Reviewer \#2: Experiments seem to have been performed in an adequate manner. A-priori analyses were carried out to ensure an adequate reliability of the ERPs. Achieved power, effect sizes and other important values are reported. Data underlying their findings is readily available. The manuscript is presented in a good English.

\*\*\*\*\*\*\*\*\*\*

6\. PLOS authors have the option to publish the peer review history of their article ([what does this mean?](https://journals.plos.org/plosone/s/editorial-and-peer-review-process#loc-peer-review-history)). If published, this will include your full peer review and any attached files.

If you chooseanalysis been performed appropriately and rigorously?

Reviewer \#1: (No Response)

Reviewer \#2: Yes

\*\*\*\*\*\*\*\*\*\*

4\. Have the authors made all data underlying the findings in their manuscript fully available?

The [PLOS Data policy](http://www.plosone.org/static/policies.action#sharing) requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data---e.g. participant privacy or use of data from a third party---those must be specified.

Reviewer \#1: (No Response)

Reviewer \#2: Yes

\*\*\*\*\*\*\*\*\*\*

5\. Is the manuscript


flow properties directly at various locations in the nozzle rather than altering the entire flow field of the jet to control momentum flux downstream of the nozzle.
Preliminary studies have indicated that effective jet noise suppression requires altering of the flow over an extended region of the jet to provide positive control over the momentum change between the jet and the induced flow of ambient air. Since the variation of the momentum flux in the jet field is related through the momentum equations to the Reynolds stresses which are the source of sound, control of static pressure in the jet field is directly related to noise suppression.
Prior art jet noise suppressors have enjoyed limited success. Devices which mechanically altered nozzle flow by modifying nozzle configuration have not significantly reducedproduce a uniform rate of change of momentum in the jet.
Another object of the present invention is to provide an apparatus and method to suppress jet noise by controlling the rate of ambient fluid entrained into the jet to produce a uniform rate of change of momentum in the jet.
Another object of the present invention is to achieve jet noise suppression through a method and an apparatus which are simple, effective, fuel efficient, lightweight, controllable, and adjustable to engine mass flow conditions.
A further object of this invention is to provide an apparatus and method to permit thrust vectoring.
A further object of this invention is to provide an apparatus and method to improve engine performance by repositioning, weakening or eliminating shock waves formed in and downstream of the engine


is straightforward. Survival matrices *S* ([5](#pone.0140024.e005){ref-type="disp-formula"}) must be obtained elsewhere.

The continuous-time model can be considered in a similar way (see Appendix A1 in [S1 File](#pone.0140024.s001){ref-type="supplementary-material"}). It also is possible to show the equivalence of both approaches (Appendix A1 in [S1 File](#pone.0140024.s001){ref-type="supplementary-material"}): for small time steps *τ* ([9](#pone.0140024.e009){ref-type="disp-formula"}) turns into the relation for the continuous-time case. Therefore both continuous and discrete time approaches are equivalent, and the choice of discrete or continuous time model is a matter only of preference.

Meaning of matrices *M* and *G* = *DMD* ^−1^ {#sec005}
--------------------------------------------

The next-generation matrix *G* can be called the matrix of secondary infections, because its entry *G* ~*ij*~ is the number of secondary infections of type *i* produced by one infected individual of type *j* during its lifetime (see e.g. \[[@pone.0140024.ref014],[@pone.0140024.ref017]\]). ThereforeWe assume that CWD prevalence in hunter-harvested deer is equal or proportional to that in the wild population. At present, there is no agreement whether a hunter harvest prevalence estimate is biased or non-biased. However, in our case, for estimating *R* ~0~, proportionality is enough. It means that the prevalence in harvested individuals and in the population is related as *i* ~*k*,harvest~ = *B* ~*k*~ *i* ~*k*~, provided the coefficients *B* ~*k*~ do not change with time (the non-biased case corresponds to *B* ~*k*~ = 1). Then it can be shown that, in the case of diagonal survival matrices, *i* ~*k*,harvest~ leads to the same *R* ~0~ estimate even if the bias is different for each deer category (when all *B* ~*k*~ are equal, the diagonality of survival


and AIC~C~ supported by the model are in line 12 of the table, which includes a culling term only for females *γ* ~2~ ≈ 3.4 × 10^−4^ and has *R* ~0~ ≈ 4.0. The corresponding matrix *F* was $$F \approx \begin{pmatrix}
1.1 & 3.3 \\
0.1 & 2.0 \\
\end{pmatrix}.$$ However, the standard error exceeded values of the parameters. This happens because the profile of log-likelihood is close to flat in some directions and large changes of parameters along them lead to small changes in likelihood. To find these directions, we found eigenvalues and eigenvectors of the Hessian matrix at the point of likelihood maximum. There were two small eigenvalues, and the direction of the one of the eigenvectors almost exactly coincided with the initial prevalence for males, while the secondfor CWD that produce the observed 2:1 prevalence in males:females. The expressions for force of infection depend on population proportions, seasonality, transmission coefficients, and the disease prevalence. Assuming reasonable values for population parameters and linearizing the expressions with respect to prevalence, we obtain the entries of matrix *F* depending on two unknown transmission coefficients; see details in Appendix A3 in [S1 File](#pone.0140024.s001){ref-type="supplementary-material"}. It can be presented as a template with two parameters to fit, similar to empirical templates in lines 2 to 11 of [Table 4](#pone.0140024.t004){ref-type="table"}. We tried six combinations of population parameters (see Appendix A3 in [S1 File](#pone.0140024.s001){ref-type="supplementary-material"}). Four of them gave close values of AIC, and one of the two models is shown in [Table 4](#pone.0140024.t004){ref-type="table"} in row 12. It appears second best in [Table 4](#pone.0140024.t004){ref-type="table"}


home range and stop the transmission among females. Indeed, Cullingham et al. \[[@pone.0140024.ref025]\] found that female deer harvested with 2 km were more genetically related than males, and CWD-positive deer were more likely to be related. In contrast, males move within larger areas than females \[[@pone.0140024.ref022],[@pone.0140024.ref026]\], and if males contact individuals over a larger area they will increase the spatial spread of the disease.

On the other hand, the conclusions about the efficiency of culls in females (but not on the number of secondary infections left by females) are based mainly on the decline in estimated female prevalence between 2006 and 2007--2008. Therefore additional data are necessary to come to a more definite conclusion.

Supporting Information {#sec017}
======================

###### Appendix A1. Basic reproduction number for continuous-time model. Appendix A2. Likelihood for hunter-survey             )
                                                    )

          BEFORE: SUHRHEINRICH, GIBBONS, and COOK, Circuit Judges.

          PER CURIAM. Matthew Brown appeals the district court’s judgment of conviction and

sentence.

          A jury found Brown guilty of possessing child pornography, in violation of 18 U.S.C.

§ 2252A(a)(5)(B), and


as ER stress inducers and staurosporine (STS) was used as a mitochondrial stress inducer. Yokukansan significantly decreased the cell death caused by TG and hypoxia ([Figure 1A and 1B](#pone-0013280-g001){ref-type="fig"}), but did not protect against STS treatment ([Figure 1B](#pone-0013280-g001){ref-type="fig"}). These results indicate that Yokukansan is effective against ER stress-induced neuronal toxicity that involves impairment of calcium homeostasis, but not apoptotic stimuli that do not cause ER stress. Notably, as shown in [Figure 1C](#pone-0013280-g001){ref-type="fig"}, the protective effect of Yokukansan against ER stress-induced cell death is proportional to the concentration of Yokukansan used. However, a high dose of Yokukansan showed some toxicity.

![Yokukansan reduces ER stress-induced neuronal cell death.\
Cell toxicity in N~2~a cells was measured based on morphological changes. Quantitative data are expressed as the mean ± SEM for at least threestress, was reduced following pretreatment with ferulic acid, as was seen following pretreatment with Yokukansan or Senkyu ([Figure 4C, D](#pone-0013280-g004){ref-type="fig"} and [7B](#pone-0013280-g007){ref-type="fig"}). These results suggest the involvement of ferulic acid in the regulation of the UPR signaling pathway.

![Ferulic acid, Senkyu and Yokukansan reduce susceptibility to ER stress by altering the unfolded protein response (UPR) signaling pathway.\
(A) SK-N-SH cells were treated with ferulic acid at the indicated concentrations (0, 10, 25 µg/ml) for 2 h. (B) SK-N-SH cells were treated with 1 µM TG with or without a 2 h pretreatment with ferulic acid at the indicated concentrations (0, 10, 25 µg/ml). (A, B) The expression of GRP78/Bip (A), CHOP (B) and GAPDH (A, B) mRNA were detected by RT-PCR. Quantitative data were obtained by densitometry of the bands.


was measured independently in 4 fields and at least 300 cells were counted. Data are expressed as the mean ± SEM for at least three independent experiments.

Cell viability assay by WST-1 activity {#s4e}
--------------------------------------

SK-N-SH cells, PS1 WT cells, or PS1ΔE9 cells (3×10^3^) were plated onto 96-well plates 36 h before cell viability was determined. Prior to performing the assay, cells were pretreated for 1.5 h with Yokukansan, or with every component of Yokukansan, at the indicated concentrations followed by the addition of each insult (TG or STS) for 3 h. Following insult exposure, cells were washed twice with phosphate buffered saline (PBS) and cultured with DMEM (D1145, SIGMA) and WST-1 mixed medium for 3 hours. WST-1 was measured at an absorption of λ450 nm -- λ650 nm. Data are		<url-pattern>/*</url-pattern>
 * </filter-mapping>
 * </pre>
 *
 * <p>
 * In the case of the tomcat cache problem, it is important that the url-pattern for
 * the filter matches the url-pattern set for the security-constraint.
 *
 * @author Mike Wille
 */
public class ResponseHeaderFilter implements Filter {
	private FilterConfig config;

	public void init(FilterConfig filterConfig) throws ServletException {
		config = filterConfig;
	}

	public void doFilter(ServletRequest req, ServletResponse resp, FilterChain chain) throws IOException,
			ServletException {
		HttpServletResponse httpResp = (HttpServletResponse) resp;

		Enumeration e = config.getInitParameterNames();

		// for each configuration element...
		while(e.hasMoreElements()) {
			String headerName = (String) e.nextElement();
			String headerValue = config.getInitParameter(headerName);
			// set the header with the given name and value
			httpResp.setHeader(headerName, headerValue);
		}
		chain.doFilter(req, resp);
	}

	public void destroy() {
	}
}


LNC population at day 7 and 42 after AAD induction in HLN (Figures [1A,B](#F1){ref-type="fig"}). This was not seen in ILN cell populations (Figures [1C,D](#F1){ref-type="fig"}). Collectively, these data suggest that PDE8A protein abundance is higher in the HLN CD4^+^ T cell population than in the HLN CD4^−^ cell population at the acute intermediate and later stage of AAD. In contrast, in both HLN and ILN, PDE8A protein expression was lower in CD4^+^ T cells as compared to the CD4^−^ LNC population at the early acute stage of AAD on day 3 (Figure [1](#F1){ref-type="fig"}). Of note, this selective expression pattern was not seen with PDE4B isoforms (data not shown). In contrast to protein expression, the highest of *pde3b, pde4b, pde7a*, and *pde8a* genes in the CD4^+^ T cell fractions fromfine. RuneQuest is a stellar example of a game that knows what it wants to do. Combat is detailed because we’re playing out a simulation that is ugly, dirty, and likely to leave everyone injured. Magic is detailed, and full of requirements and considerations and options for ritual add-ons because you’re entreating spirits or gods to show you favor. The rules aren’t there to obscure the moment: they’re there to highlight it. So while I won’t reach for RuneQuest for Fast! Furious! Fun! I will use it when I want to tell a tale of heroes that reads like the Literary Epics rather than a Marvel Cinematic Universe movie. **Downsides** Not everything is perfect though. RuneQuest Roleplaying in Glorantha is a fantastic product, but it does feel incomplete.


About Us...

USA Today Bestselling Authors Nikoo and Jim wrote their first May McGoldrick romance using historical figures that Jim researched while earning a PhD in sixteenth-century Scottish and English literature. Nikoo, a mechanical engineer, is a born storyteller. She is all about characters and feeling. Jim is about action and sense of place. Together, they have crafted over forty fast-paced, conflict-filled historical and contemporary novels and two works of nonfiction under the pseudonyms May McGoldrick and Jan Coffey.

Their stories have touched the hearts and minds of millions of readers all over the world. Their work has been translated into over a dozen languages, and counting. Because of their success, they have been frequent guests on local and national radio and television. They travel regularly, speaking at writers’ conferences,105 Ind. 456, 5 N.E. 171; Burkhart v. Gladish (1889), 123 Ind. 337, 24 N.E. 118 (exception stated); Mitchell v. Walton (1922), 192 Ind. 193, 133 N.E. 496; Kennedy v. Kennedy (1922), 192 Ind. 353, 136 N.E. 557 (exception limited).
161 Ind. App. at 456-57, 316 N.E.2d at 397. See also Loeser v. Simpson (1942), 219 Ind. 572, 39 N.E.2d 945; Hottenstein v. Hottenstein (1922), 191 Ind. 460, 133 N.E. 489. Thus, under our standard of review, since Louise would be incompetent to testify at trial, her affidavit cannot be received and considered to defeat the motion for summary judgment. It is significant that in her affidavit she made no contention that the decedent was of unsound mind, an exception to the rule recited in Bechert, supra.
Conceding the rule,


of Newburgh v. Jones (1945), 115 Ind. App. 320, 58 N.E.2d 938.
There appears to be some departure from the above rule, however. In Duncan v. George Moser Leather Co. (1980), Ind. App., 408 N.E.2d 1332, this court set forth a three-prong test to determine the admissibility of expert opinion when it is based in part on a report that is either not in existence or inadmissible as substantive evidence due to the hearsay rule. In order for the opinion to be admissible the following standards must be met: (1) the expert must have sufficient expertise to evaluate the reliability of the report; (2) the report must be of a type normally found reliable; and (3) the report must be of a type customarily relied upon by the expertbeen working on a bill on body parts transplantation among family members. “We have drafted a temporary procedure for body parts transplant,” he said.

He said a private hospital in Herat province also started kidney transplantation in the country, a process he called as successful initiative.

UN protocol against Trafficking in Persons

Trafficking in human beings for the purpose of organ removal is encompassed within the definition of trafficking set out in the Palermo Protocol to the 2000 UN Convention which states that “Trafficking in persons shall mean the recruitment, transportation, transfer, harbouring or receipt of persons, by means of the threat or use of force or other forms of coercion, of abduction, of fraud, of deception, of the abuse of power or of a position of vulnerability or of the


After dicing up the chicken cutlets, place them in container and sprinkle 1 tsp each: salt, pepper, cinnamon, cumin, and coriander. Top with juice of 1 lime, as well as grated lime zest. To finish top everything 1 tablespoon of the minced garlic and some fresh cilantro. Shake container and place in fridge for 30 minutes.

While chicken is marinading, begin preppeing veggies, broth and beans.

While soup is cooking, grab the chicken from the fridge and in a large skillet coated with cooking spray begin cooking. Top chicken with additional garlic, squeeze the second lime over the chicken as it simmers, and top with more fresh cilantro. Allow chicken to cook through, outside should be golden and crisp.

Once veggie pot is at a boil, reduce heat to low simmer.the place that you’re inquiring about, for fear that they’ll see you.

Paranoid motherly digressions and self-conscious age admissions aside, it was a joy being a part of this crowd. Even though I got a few strange looks the first day because I wore a tank top and shorts (Coachella attire) to the Superdome, once I had proven myself worthy by knowing words to songs I shouldn’t know the words to, I felt as if I had been pardoned and was allowed to stay with the party people.

I should have done better, though, with my clothing. I knew better. When I packed my bag, I threw in a blazer, tie, and slacks, but then laughed at myself because it was supposed to be 90 degrees in NOLA, and proceeded


Awards. When it comes to favorite artists of my childhood, especially those with classic albums, I’m often hesitant to hear new, “experimental” music for fear that it will ruin the illusion of perfection surrounding the artist. This is the well-documented story of post-Miseducation Lauryn Hill, so when news broke that D’Angelo was back with new music, I was nervous.

All nerves have since subsided, because he’s unbelievable live. The unfortunate thing, however, is that I was in the minority that felt this way Friday night. For the first 45 minutes of his set, there were scattered people standing up jamming to his set, but most were sitting, almost as if this new music, filled with loud guitar and other rock influences, was an unpleasant experience.

Thing I Learned About EssenceKanye’s “All of the Lights” and Snoop Dogg’s “Beautiful.” He’s a legend who has found a way to stay relevant for more than three decades. And with all of that known, I still, rudely, wanted to leave. But I didn’t, because the moment he was announced onstage, the ENTIRE crowd rose to their feet, yelling at the top of their lungs.

Thing I Learned About Essence Concertgoers No. 2: They just want to dance. I can’t be mad at them for not vibing with D’Angelo, because it’s not that they hated it, it’s just that they couldn’t really dance to it. This isn’t a bebopping crowd. This is a hands-up-in-the-air, two-stepping, line-dancing group of people trying to party as hard as they have since Essence 2011.

Charlie gave them the


Aretha walked offstage and a long photo montage of Aretha’s career landed on the monitors, complete with interviews and testimonials. After that, a few people, including the editor-in-chief of Essence, the lieutenant governor of Louisiana, and the mayor of New Orleans, came out to talk more about her and give her the key to the city, as well as some other accolades. After this, Aretha came back onstage and, not knowing that this long tribute has taken place, introduced them and told them to come onstage because they had something to give to her.

Oh, Aretha.

So they come back onstage, do their best job at pretending like this is the first time this has happened, as does the crowd, who clap loudly for a second time. Hilarious.

But that’s notin 10 years, then yes. I’m there. 100 percent.

THE ESSENTIAL ESSENCE PLAYLIST (most commonly heard songs played between sets, out of car stereos, in bars, and sung on the streets of New Orleans):


though Southall agrees Friends of Coal might support large mining corporations which do not always have Appalachian interests at heart — her brother became a paraplegic and her fiancé was killed in two separate mining accidents — she insists that locally the group works to protect the culture, families, and future of Appalachian citizens. Southall is not alone. Throughout central Appalachia, residents at once seemingly smarted by the coal industry — through ill health, unstable employment, or desecrated land — also call themselves ardent friends of coal. Through the Friends of Coal campaign, the corporate coal lobby has managed the impossible: to reinvent the coal industry as an ally and compatriot of Appalachia’s working class. And, as the coal lobby’s presence deepens in the region, its residents’ futuresand declared it permanent, though Maggard was unable to receive compensation since no one was hurt. Maggard used to have two hundred miners in his store every day. Now, he can’t even afford to fully stock his shelves. It’s the Obama-era environmental regulations and “wind-up-doll” politicians that have torn apart the coal industry and fractured the local economy, Maggard believes. The only thing that can save him is coal mining, and the foolhardy politicians intent to keep the industry alive. “It’s there. It’s not like it’s gone,” he insists. “Coal is there. And why not use it?” Maggard’s store sits on the border of Lynch, once the biggest coal-mining town in the world and a model for how unrestrained industry could nourish America’s rural communities. Today, these riches


more than just an impression. It’s a reality. Under the Friends of Coal brand, communities organize breast cancer fundraisers and charity drives for the local church. One section of the Coal in Classroom program takes children to the local retirement home to sing and speak to its residents. Often, it’s difficult to identify where Friends of Coal’s corporate marketing ends, and the sincere desires of a town desperate for employment and community spirit begin. In this tangle of industrial and communitarian loyalties, the ideals of a social justice movement can seem like an anathema to an Appalachian identity. And, yet, there is a small constellation of environmental and social groups that call these mountains home and openly criticize Friends of Coal’s agenda. Their calls for investment in renewableenergy loses. This is not always a straightforward issue since there are a wide variety of potential electrode materials \[[@B10-materials-10-00079]\]. Thus, when developing new BES, having a fast and easy method for the screening and characterization of electrode materials could become a powerful tool that can result in substantial time and resources savings.

The presence of surface patterns on electrodes is a key aspect when selecting electrode materials for BES, as it has a significant effect on their electrochemical performance \[[@B11-materials-10-00079]\]. The electroactive area (EA) of an electrode is a parameter clearly related to its surface structure, and its determination represents an essential step in characterising the electrochemical behaviour of electrodes in electrochemical systems in general \[[@B12-materials-10-00079]\] and BES in particular \[[@B13-materials-10-00079]\]. By combining EA determination with other analyses


0.1 M and it was obtained from bibliographic data \[[@B19-materials-10-00079]\].

Cathodic peak currents (*I~pc~*) can be obtained from CV experiments using the decaying anodic current as baseline \[[@B18-materials-10-00079]\]. From the slope of the linear fit between *I~pc~* and the square root of the scan rate, the electroactive area can be determined, following Equation (1). This approach can only be applied in the experimental range in which a linear trend is observed between *I~pc~* and *υ*^1/2^. The described approach is used for comparative purposes, keeping in mind that this model applies for flat electrodes \[[@B18-materials-10-00079]\].

Although more accurate numeric treatments have been developed \[[@B14-materials-10-00079]\], this study implements a simple model for the wide range of materials tested.

2.3. Determination of Fractal Dimension (D~f~) {#sec2dot3-materials-10-00079}
----------------------------------------------

Roughness is a key parameter in electrode behaviour becausetheir nature, skip the more metaphysical and personal elements of the process.

Now, a movement to restore lost tradition has motivated a new generation of Jewish volunteers to learn a set of skills that was common knowledge for many of their great-grandparents: the rituals of bathing, dressing and watching over the bodies of neighbors and friends who have died.


MOSCOW (Sputnik) — Iraqi Kurdistan Region will hold referendum on independence in 2017, and will establish two special boards to prepare for the procedure, the secretary of the Kurdistan Democratic Party (KDP) politburo said on Tuesday, as cited by the Kurdish Basnews website.

One of the boards will be tasked with internal preparation for the vote, while the second will act as an executive body and cooperate with the Independent High Electoral Commission (IHEC) and the Kurdistan Regional Government (KRG), Fadhil Mirani told reporters at a press conference after the meeting of the KDP and the Patriotic Union of Kurdistan’s (PUK) politburos.

Mirani stressed that the announcement does not mean that Kurdistan would proclaim independence shortly after the vote, but stressed that the regional authorities "do not ask for the200 and 300 has an inverting input (-) and a non-inverting input (+). These amplifiers 200 and 300 are energized by a power supply source Vcc. A first input signal V.sub.S1 is applied to the non-inverting input (+) of the amplifier 200. A series connection of a resistor R.sub.f1 and a capacitor C1 is connected between the inverting input (-) of the amplifier 200 and ground to form a feedback circuit together with the feedback resistor R.sub.f3. An output terminal of the amplifier 200 is connected to a first load R.sub.L1 through a capacitor C.sub.3. Similarly, a second input signal V.sub.S2, a resistor R.sub.f2, capacitors C.sub.2 and C.sub.4 and a second load R.sub.L2 are connected to the amplifier 300.
The amplifiers 200 and 300 amplify respectively, the first and


citizens to call Congress and tell them they want the border secured.

Trump’s comments came about a week after he noted that the house of former President Barack Obama has a wall around it.

While Pelosi has said a wall is immoral, Senate Minority Leader Chuck Schumer (D-N.Y.) admitted recently that while he’s against a wall, a barrier will work along the border.

“A fence works. You ask the experts,” he told CBS 2. “A big, concrete wall is expensive and it doesn’t work. You don’t just need fencing. You need drones, you need helicopters, and you need roads. The agents can’t get people crossing the border because they can’t get there.”

President Donald Trump (C) is shown border wall prototypes in San Diego, Calif., on March 13, 2018. (Mandel Ngan/AFP/Getty Images)

Inpossible the ‘routers’ have
to be connected, in other words, they have
to be positioned side by side.
Moving a ‘controller’ counts also as one
of four actions, regardless the distance.
It is possible to call back a ‘controller’
to be used elsewhere, and thus giving up your
previous claimed tower.
You place it on one of your ‘routers’,
this however uses up all four actions, in other words your entire turn.
The optional escape rule can be used here
as well when called applicable.
Beware, it does allow you to rotate a
tower claimed by one of your opponents as well.
one… two…
and finished.
This is another possible starting position
for three players, playing with five … or
seven ‘routers’, you could also decide
to start with seven and only finish with five ‘routers’,
this way you could also avoid
needing the escape rule.
Starting with two players


For these analyses the nonparametric Kruskal-Wallis test with the Dunn-Bonferroni multiple comparison test were used. To assess correlations between scores in PARS and WHOQOL-BREF, Spearman\'s rank correlation coefficient was used.

Results {#S0008}
=======

Patient demographics {#S20009}
--------------------

All children with cardiac disorders included in the study underwent electrophysiological study (EPS), on the basis of which the following forms of SVT were diagnosed: AVRT (61.1%), AVNRT (33.3%), AET (3.9%). One child was diagnosed with persistent junctional reciprocating tachycardia (PJRT). In 2 patients with SVT, previously confirmed with ECG, arrhythmias were not induced during the EPS study.

The mean ± SD age of the first SVT episode was 10 ±4.6 years. In the SVT group clinical symptoms were reported by 89.4% of children, while the remaining patients were asymptomatic. In the SVT group antiarrhythmic drugs wereactual compile log go? How can I get to the whole thing?

A:

It sounds like you're trying to use the UI mode when you were happy with the command line mode in Play 2.2; the command line mode is still there, see http://typesafe.com/blog/typesafe-activator---an-update-and-roadmap-preview for some background. Just run "activator" instead of "activator ui". The UI mode is not something that's "production hardened" yet, it is primarily intended for tutorials (though we are working to change that).
That said the compile log should not be truncated in the UI and we appreciate bug reports at https://github.com/typesafehub/activator/issues including one about this truncation.


Using a robot in the classroom may sound too sci-fi. The truth is that these are being increasingly found in education. In Malta, robotics has been introduced in computing where students choosing this option subject will be using this tool as an aid for their form 3 studies. NXT Mindstorms kits are already available in all government secondary schools and more will be introduced this scholastic year. In form 3, students will be working on a practical task. Students need to see that they use a number of sensors from those available. Such sensors will serve as input and output devices which will help in the completion of the selected task.

Robots allow some autonomy of action based on their programming. Studies show that students engaged with robotics developcreated by device manufacturers to protect the privacy of their users.

From the outset our goal was to use only decentralized technology such as Ethereum and IPFS. We specifically never want to be the holders of our users’ private data.

It isn’t always possible in today’s world of Android and iOS to be 100% decentralized. Whenever we have had a problem that needs solving, we have always been very careful to make our solutions as decentralized as possible, to avoid sitting on lots of private data.

It is with this goal of providing truly decentralized services in mind that we wanted to explain how we think about these issues and how we attempt at solving them. This article is going to be fairly long as privacy and self-sovereignty is really important


was waived by the Ethics Committee, consistent with the South African Medical Research Council's Guidelines on Ethics for Medical Research and the Declaration of Helsinki, because this was a retrospective analysis of routine clinical service records, no additional data collection or procedures were undertaken from or on patients, all patient information was entered into the database using coded identification numbers, and no information that could reveal patient identity was entered into the database.

Results {#s3}
=======

Study Subjects and Baseline Characteristics {#s3a}
-------------------------------------------

We identified 552 TB patient records during the study period, of which 343 did not meet the inclusion criteria ([**Figure 2**](#pone-0046988-g002){ref-type="fig"}). Of the remaining 209 potential study participants, 21 (10.0%) were excluded because eligibility could not be assessed. There were no statistically significant differences in sex, age, TB patient category,whether transmission in those patients occurred at the facility level. The authors stressed that HIV patients with unknown TB infection pose the greatest risk of transmission in high HIV prevalence settings, and that integration reduces this risk through active case finding leading to earlier identification and treatment of TB. HIV-infected patients in our study were screened for TB at every visit. Moreover, diagnosed and undiagnosed HIV and TB patients also come into close contact in non-integrated clinics and in community settings (taxis, halls, homes and others), especially in high prevalence settings [@pone.0046988-Wood1]. Thus infection control is crucial in TB and HIV high prevalence areas regardless of the level of health care integration. Whilst implementation of infection control measures is the responsibility of clinic staff, it requires a targeted


focus for measures to be consistently implemented. Increased focus on infection control throughout the health sub-district of Khayelitsha was made possible thanks to the addition of an infection control officer.

Strengths and Limitations {#s4a}
-------------------------

Randomised controlled trials are the gold standard design for evaluating complex interventions, but are not always possible because of practical, ethical, financial or methodological difficulties [@pone.0046988-Simon1], [@pone.0046988-Craig1] and may not accurately reflect an intervention's feasibility or effectiveness in a real-world setting (i.e. have limited applicability) [@pone.0046988-Treweek1]. To evaluate an intervention that had already been implemented, we chose a before/after study design and used several strategies to reduce the weaknesses of this design to limit bias and confounding [@pone.0046988-Craig1]. One strategy was to use a short (6 month) observation period before and after the intervention to limitas by observational data [@pone.0046988-Franke1].

Finally, this study is limited by the small sample size and the fact that it was implemented in only one site in an urban South African township. The findings need to be confirmed by larger studies in different settings.

Conclusions {#s4b}
-----------

In this study TB/HIV integration resulted in immediate benefits for patients. The study further demonstrates the feasibility of integration in a peripheral primary care clinic using a nurse-led model with limited support from medical doctors. The setting is representative of many resource-constrained primary health care settings facing dual HIV and TB epidemics. Our findings add to the body of evidence supporting the rapid scale-up of service integration in settings with a high TB/HIV co-infection burden.

Supporting Information {#s5}
======================

######

**Directed Acyclic Graph.** The following steps were used


and customers’ great favor for modern and fashionable floor lamps for reading. All this beauty is not just decorating and illuminating the interiors of hotel or palace rooms, halls theaters and restaurants, halls or boudoirs of private […]

It is simply amazing how different a room and even the whole house can look like when you decide to change the décor in such small and not so basic details as the lighting. Adding some new lamps, no matter whether it going to be another floor one or some new table lamps for the office room or for your […]

The ceiling lamps are the most natural representatives of illumination system. They are capable to create the effect of on natural light. It is hidden in its technology. The light for// Copyright 2014 The Chromium Authors. All rights reserved.
// Use of this source code is governed by a BSD-style license that can be
// found in the LICENSE file.

#ifndef COMPONENTS_TRANSLATE_CORE_COMMON_TRANSLATE_CONSTANTS_H_
#define COMPONENTS_TRANSLATE_CORE_COMMON_TRANSLATE_CONSTANTS_H_

namespace translate {

// The language code used when the language of a page could not be detected.
// (Matches what the CLD -Compact Language Detection- library reports.)
extern const char* const kUnknownLanguageCode;

}  // namespace translate

#endif  // COMPONENTS_TRANSLATE_CORE_COMMON_TRANSLATE_CONSTANTS_H_


admito, sou a favor de políticas pró #Ocupação
- Espera aí, eu esperava que você dissesse
que as ocupações deveriam acabar ...
- Acabar? Nós vimos #Ocupando há séculos,
sob a terra, o mar e o ar.
Nós #Ocupamos a América do Norte,
a #Austrália e o #Hawai.
e se vocês se esqueceram disso,
é por que nós também #Ocupamos a História.

Dutch:
- hebben één eis voor te leggen  - voor te leggen
- duidelijk gezegd  - duidelijk gezegd
het regime van buitenaardse rechtspersonen eindigt hier
- Ben jij de leider?  - Nee. We wij zijn een beweging van vrijdenkers,
zonder hiërarchische structuur  - wij zijn een beweging van vrijdenkers--
- Zwijg!
- Ik vraag me af: als je voor vrije informatie bent, waarom dan het masker?
- Omdat ik slechts een idee ben, wiens tijd eindelijk isbaseline characteristics ([S4 Table](#pone.0160768.s005){ref-type="supplementary-material"})--chosen because of their potential influence on the outcomes or segregation of patients between the compared groups.\[[@pone.0160768.ref049]--[@pone.0160768.ref051]\] By applying propensity score-based weights to the patients and outcomes, we created weighted groups that were well-balanced on all measured baseline characteristics. We compared baseline characteristics between the groups using standardized differences. This metric describes differences between the group means relative to the pooled standard deviation---a difference greater than 10% is considered meaningful.\[[@pone.0160768.ref048], [@pone.0160768.ref052]\] We calculated relative risks (RR) with 95% confidence intervals (CI) using log-binomial regression models accounting for the weights.

We also evaluated the association for both exposed groups (citalopram and escitalopram) with our outcomes in pre-specified subgroups of patients---defined by the presence or absence of: 1) congestive heart failure, 2) coronary artery disease, 3) chronic kidney


non-standard daily doses of the SSRI). The mean age was 76 years old (range 66 to 105), and 66% were women. General practitioners wrote 78% of the prescriptions. The distribution of baseline characteristics before and after propensity score weighting are presented in [Table 1](#pone.0160768.t001){ref-type="table"} for citalopram and [Table 2](#pone.0160768.t002){ref-type="table"} for escitalopram.

10.1371/journal.pone.0160768.t001

###### Baseline characteristics for the citalopram cohort (pre and post weighting).

![](pone.0160768.t001){#pone.0160768.t001g}

                                                                   fact that the integral of an odd function (i.e., $\frac{\cos \omega t}{\omega}$) about symmetric limits is zero.
In going from $(2)$ to $(3)$, we exploited the fact that the integral of an even function (i.e., $\frac{\sin \omega t}{\omega}$) about symmetric limits is twice the integral over one half interval.
In going from $(3)$ to $(4)$, we enforce the substitution $\omega \to \omega/t$.
And in going from $(4)$ to $(5)$, we use the well-known value of the Dirichlet Integral.


Q:

I would like to store the results of a query, then compare those results to the next set. Possible?

//This is my query
SELECT bline_id, ROUND(Avg(flow),3) avg
FROM   (SELECT id, bline_id, flow, date, CASE
             WHEN @previous IS NULL
               OR @previous = bline_id THEN @rownum := @rownum + 1
             ELSE @rownum := 1
           end rn,
           @previous := bline_id
    FROM  ###### Article summary

Article focus
=============

-   There is preliminary evidence that partial sick leave may enhance return to work, nevertheless the effects of partial sick leave on the use of disability pension remain largely unknown.

-   The aim was to estimate the association between partial sick leave and disability pensions applying propensity score (PS) methods.

Key messages
============

-   Partial sick leave reduced the risk of full disability pension by 6% and increased the risk of partial disability pension by 8% compared with full sick leave; however, there was no effect on the total rate of disability pensions.

-   Since the majority of those on partial disability pension work part-time, results suggest an increase in work retention.

Strengths and limitations of this study
=======================================

-   A register-based prospective


limited in number.[@R2; @R3; @R4; @R5; @R6; @R7] Although the indicators of outcome are inconsistent across these studies, they suggest that the use of partial sick leave enhances RTW. Little evidence exists on the effects of partial sick leave on transition to disability pension.[@R4]

In Finland, roughly one-third of the working-age population has a longstanding health problem affecting work ability.[@R8] The proportion is one of the highest in OECD-countries. A little more than half of the full sickness benefit periods in Finland are granted to women. In contrast, the incidence of disability pensions is in general 11% higher among men than among women. The more frequent use of partial sickness and disability benefits among women (around 70% of the total) than men may be to some extent explained byoccupational gender segregation: women work more frequently in occupations where combining work with disability benefits is plausible. Musculoskeletal and mental disorders are leading causes for both sickness benefits and disability pensions.

In evaluating the effects of a health policy (or a treatment or a conduct), a controlled trial with random assignment (RCT) of subjects into treatment and control conditions is desirable in order to obtain unbiased estimates. A shortcoming of RCTs is the use of selected samples often of small size, which limits the applicability of the results outside the study population. In addition, it has been recognised that randomisation is not always successful in eliminating differences in characteristics between the study groups.[@R9] Observational studies can better represent real-world settings than RCTs. However, a concern is that the treatment


72 days. In the control group, due to our sampling criteria, the full sick leave period must have ended with a continuous phase of at least 60 days of payment and the maximum length of the period is 300 days. This resulted in discrepancy of the covariates indicating length of sickness absence prior to and in connection with treatment assignment in the studied groups. Thus in order to make the variables comparable in the studied groups, the variable indicating length of sickness absence before treatment assignment was modified in the partial sick leave group by subtracting 60 days from the original variable. Respectively, the length of sick leave in connection with treatment assignment was divided by two in the partial sick leave group (to make the amount of sick leave comparable between PS matching‡ (n=1456)      0.6 (0.4 to 0.8)          3.1 (2.0 to 4.7)
  Mental disorders
   Crude (n=7003)             0.3 (0.2 to 0.4)          3.2 (2.3 to 4.5)
   Adjusted\* (n=7001)        0.6 (0.4 to 0.8)          3.2 (2.1 to 4.8)
   Adjusted† (n=7001)


current findings with those from other studies is not possible, though it has been suggested that different measures of RTW are highly correlated and comparable.[@R23] The overall results of this study, suggesting enhanced work retention after partial sick leave, are in agreement with a Danish study showing beneficial effects of graded RTW on work participation.[@R3] Our results concerning musculoskeletal disorders are congruent with Swedish findings and a recent Finnish RCT indicating beneficial effect of partial sick leave on earlier RTW.[@R6] [@R7] Our findings showed a weaker effect of partial sick leave in the prevention of full disability pension and a remarkably increased risk of partial disability pension in mental compared with musculoskeletal disorders. The only previous study---that we are aware of---on the effects of partial sick leave inbias caused by selection into treatment. Although criticised, covariate adjustment using PS has been the most frequently used PS method in health research.[@R31] We used two different approaches---covariate adjustment with linear PS and PS matching. Stratification could not be used in the present study because of the relatively small sample size in the partial sick leave group. As in previous studies[@R20] [@R31] we found that the degree of bias reduction depended on the methodology used in PS analyses, that is, matching resulted in a more exact covariance balance between the studied groups and thus improved exchangeability between them. As removing unmatched subjects reduced data to the sample of those individuals who either had been granted partial sickness benefit or were equal to them on the measured covariates, the


results of the PS matching cannot be generalised to the whole eligible population.

In general, regression analysis as a method is sensitive to the relative difference in the sample size between the treated and untreated. That is, if the prevalence of the outcome is lower in the larger, non-treated group, the treatment effect will be underestimated.[@R12] In the present study, the number of non-treated (subjects on full sick leave) exceeded by 10-fold the number of treated (those on partial sick leave). The prevalence of partial disability pension was roughly three times lower among the non-treated as compared with the treated. As expected, the ORs for partial disability pension estimated by covariate adjustment with PS were lower than the ones estimated using PS matching. Thus, PS adjustment is more likelyAuthor\'s manuscript

###### Reviewer comments

**Contributors:** JK, SS and EVJ designed the study. JK and SS conducted the data analyses and drafted the manuscript. All the authors critically commented and revised the manuscript.

**Funding:** Funding from the National Insurance Institution (grant number 67/26/2011) and the Academy of Finland (grant number 129364) is acknowledged.

**Competing interests:** None.

**Ethics approval:** Ethical approval was not necessary, since we used only encrypted register data and did not contact the unidentifiable study subjects.

**Provenance and peer review:** Not commissioned; externally peer reviewed.

**Data sharing statement:** No additional data are available.


La herida del tiempo

La herida del tiempo (English title:The wound of time) is a Mexican telenovela produced by Televisa and transmitted by Telesistema Mexicano.

Cast
Miguel Ángel Ferriz Sr.
Angelines Fernández
Miguel Córcega
Aurora Molina
Graciela Döring
Rafael del Río
Jose Antonio Cossi
Emma Arvizu

References

Category:Mexican telenovelas
Category:1962 telenovelas
Category:Televisa telenovelas
Category:1962 Mexican television series debuts
Category:1962 Mexican television series endings
Category:Spanish-language telenovelasHTC U is in the news for past few months now, and we have seen a lot of leaks of the device that include the press render, the official introduction video and more. One of the things that were not known till now is the spec sheet and today, we have got the exclusive leak from MySmartPrice that reveals every single feature of the upcoming HTC U ( or U11). Some specs like the processor and RAM are already known from the previous leaks as the device was spotted on Geekbench a couple of days ago, but some other features like the touch-sensitive frame, etc. have been confirmed today.

HTC U SPECIFICATIONS – HERE IS EVERYTHING YOU NEED TO KNOW

The phone is going to launch on May 16th, and


Is the ataxia of Charlevoix-Saguenay a developmental disease?
The autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) is considered a neurodegenerative disease caused by mutations in the SACS gene, located on chromosome 13q12.12. It is a syndrome that comprises skeletal, retinal and neurological manifestations, among which feature spasticity, cerebellar ataxia and peripheral neuropathy. Five patients with a molecular diagnosis of ARSACS underwent clinical, radiological, and ophthalmologic examinations. Every one of the identified causal mutations was novel. Spastic ataxia, peripheral neuropathy, pes cavus, and hammertoes were found in every case. T2 and T2-fluid attenuation inversion recovery-weighted MRI sequences demonstrated cerebellar atrophy and a hypointense linear striation at the pons. Tensor diffusion sequences revealed that the hypointense striation corresponded with hyperplasia of the pontocerebellar fibres, which gave place to abnormally thick middleoverall background density of the drawing. The overall illumination of the easel is then adjusted to produce the requisite average amount of reflected light at the area being measured, depending on such factors as film sensitivity, original document contrast, and image density.
Large construction drawings generally have severe variations in image density, due to the presence of both large open areas and areas having a predominance of fine line data. Thus, with prior art microfilm systems it is usually necessary to record the drawing on several film frames having different illumination levels in order to capture all the required detail. This makes the viewing of the information cumbersome to the user since he or she must use several frames to examine all areas of the drawing. This limitation further


have a greater capacity for high speed document recording, and wastes a good deal of space around the imaging system, the wasted space being used only to pile up completed documents.
One aspect of this invention is based on the problem of highly variable image density on large format construction drawings. Another aspect of the invention is directed to improving handling of original documents after filming, also known as document off-loading.is trivial. Hence any compact $h$-cobordism of dimension $\ge 6$ with $G$ as fundamental group is trivial.

-   *Kaplansky Conjecture for characteristic zero.*\
    If $R$ is a field of characteristic zero or if $R$ is a skew-field of characteristic zero and $G$ is sofic, then $RG$ satisfies the Kaplansky Conjecture.

Searching for counterexamples {#subsec:Searching_for_counterexamples .unnumbered}
-----------------------------

There is no group known for which the Farrell-Jones Conjecture, the Farrell-Jones with coefficients or the Baum-Connes Conjecture is false. However, Higson, Lafforgue and Skandalis [@Higson-Lafforgue-Skandalis(2002) Section 7] construct counterexamples to the *Baum-Connes-Conjecture with coefficients*, actually with a commutative $C^*$-algebra as coefficients. They describe precisely what properties a group $\Gamma$ must have so that it does *not* satisfy the Baum-Connes Conjecture with coefficients. Gromov [@Gromov(2000)] constructs such a group $\Gamma$ as a colimit over


close to the identity and homotopic to the identity via homotopies whose tracks are small. Here “close” and “small” are understood to be measured in $N$ considered as a metric space. In particular it says that a homotopy equivalence which is controlled enough represents the trivial element in the Whitehead group.

Controlled topology and its variations have been important for a number of further celebrated results in geometric topology. Some of these are concerned with the Novikov conjecture [@Carlsson-Pedersen(1995a)], [@Farrell-Hsiang(1981)], [@Ferry-Weinberger(1991)], [@Hu(1995)], [@Yu(1998a)], ends of maps [@Quinn(1979a)], [@Quinn(1982a)], controlled $h$-cobordisms [@Anderson-Munkholm(1988)], [@Quinn(1987)], Whitehead groups and lower $K$-theory, [@Farrell-Hsiang(1981b)], [@Farrell-Jones(1986a)], [@Farrell-Jones(1987d)], [@Farrell-Jones(1998)], [@Hu(1993)], topological rigidity [@Farrell-Jones(1988b)], [@Farrell-Jones(1990b)], [@Farrell-Jones(1993d)], [@Farrell-Jones(1998)], homology manifolds [@Bryant-Ferry-Mio-Weinberger(1996)], parametrized Euler characteristics and higher torsion [@Dwyer-Weiss-Williams(2003)] and topological similarity [@Hambleton-Pedersen(2005a)]. Of course this list is not complete.

A key theme indimensional cover of $Z$ by open sets. Recall that points in the realization of the nerve $| {\mathcal{U}}|$ are formal sums $x = \sum_{U \in {\mathcal{U}}} x_U U$, with $x_U \in [0,1]$ such that $\sum_{U \in {\mathcal{U}}} x_U = 1$ and such that the intersection of all the $U$ with $x_U \neq 0$ is non-empty, i.e., $\{ U \; | \; x_U \neq 0 \}$ is a simplex in the nerve of ${\mathcal{U}}$. There is a map $$\begin{aligned}
 && f = f^{{\mathcal{U}}} \colon Z \to | {\mathcal{U}}|, \quad x \mapsto \sum_{U \in
  {\mathcal{U}}} f_U (x) U,
\label{fcalu}\end{aligned}$$ where $$f_U (x) = \frac{a_U (x)}{\sum_{V \in {\mathcal{U}}} a_V (x)} \quad \mbox{ with
} \quad a_U(x) = d (x , Z-U ) = \inf \{ d(x,u) \; | \; u \notin U


which contains $C^{\prime}$ as an (honest) retract. Since the homotopy relation is transitive this proves the inclusion “$\subset$”.

Suppose we have $C \xrightarrow{i} D \xrightarrow{r} C$ with $r \circ
i \simeq \operatorname{id}$, where $C$ lies in $\operatorname{ch}^{\geq} \operatorname{Idem}( \overline{{\mathcal{O}}}
)$ and $D$ in $\operatorname{ch}_{\operatorname{f}} {\mathcal{O}}$. Then the proof of Proposition 11.11 in [@Lueck(1989)] yields a chain complex $D^{\prime}$ in $\operatorname{ch}^{\geq} {\mathcal{O}}$ which is chain homotopic to $C$ and of a special form. Namely, there exists an $n \in {\mathbb{Z}}$ and an object $D^{\prime}_{\infty}$ such that $D^{\prime}_m = D^{\prime}_{\infty}$ for all $m \geq n$. Moreover there exists a map $p \colon D_{\infty}^{\prime} \to
D_{\infty}^{\prime}$ with $p \circ p = p$ such that the chain complex is $2$-periodic above $n$ and of the form $$\dots \xrightarrow{1-p} D_{\infty}^{\prime} \xrightarrow{p}
D_{\infty}^{\prime} \xrightarrow{1-p} D_{\infty}^{\prime}
\xrightarrow{p} D_{\infty}^{\prime} = D_n^{\prime} \to
D_{n-1}^{\prime} \to$(A {{\otimes}}C_*(\delta(n)))_{n \in {\mathbb{N}}}$ is a well defined object in $\operatorname{ch}_{\operatorname{hfd}} {\mathcal{O}}^G ( E, ( G {{\times}}\overline{X}, d_{C(n)})_{n \in {\mathbb{N}}} )$.

On morphisms a problem occurs. We would like to map the morphism $\varphi \colon A \to B$ with components $\varphi_{(g',e',t'),(g,e,t)}
\colon A_{(g,e,t)} \to B_{(g',e',t')}$ to the morphism $(\varphi
\otimes l ( n) )_{n \in {\mathbb{N}}}$ whose components are given by $$(\varphi \otimes l (n))_{(g',x',e',t'),(g,x,e,t)} =
\varphi_{(g',e',t'),(g,e,t)} \otimes l(n)_{(g',x'),(g,x)},$$ with $$l(n)_{(g',x'),(g,x)} = \left\{ \begin{array}{lll} l(n)_{g'^{-1}g}
    & & \mbox{ if } x'=x;
    \\
    0 & & \mbox{ if } x' \neq x,
\end{array}\right.$$ where $l(n)_{g'^{-1}g}$ is the map $$(l_{g'^{-1}g})_{\ast} \colon C_k^{\operatorname{sing}, \delta(n)} ( \overline{X}
, d_e )_{g^{-1} x} \to C_k^{\operatorname{sing}, \delta(n)} ( \overline{X} , d_e
)_{g'^{-1} x}$$ which is induced by left multiplication with $g'^{-1}g$, i.e., a


dominated object follows from Lemma \[lem:about-support-over-GX\]. Hence $(A \otimes C_{\ast}( \delta^k (n) ))_{n \in {\mathbb{N}}}$ is indeed a well defined object. Lemma \[lem:choice-of-delta-n-k\] \[lem:choice-of-delta-n-k:increasing\] assures that one has the horizontal inclusions. The vertical maps exist because of Lemma \[lem:choice-of-delta-n-k\] \[lem:choice-of-delta-n-k:d\_and\_delta\]. Because the $E$-coordinate is left unchanged in this construction, the equivariant continuous control condition is preserved.

\[lem:choice-of-delta-n-k\] Let $(C(n))_{n \in {\mathbb{N}}}$ be a monotone increasing sequence of numbers. There exists a collection of numbers $\delta^k (n) > 0$ with $n$, $k \in {\mathbb{N}}$, such that the following conditions are satisfied.

1.  \[lem:choice-of-delta-n-k:increasing\] For every fixed $n \in {\mathbb{N}}$ the sequence $(\delta^k(n))_{k \in {\mathbb{N}}}$ is increasing, i.e., $$\delta^1(n) \leq \delta^2 (n) \leq \delta^3 (n) \leq \dots  ;$$

2.  \[lem:choice-of-delta-n-k:alpha(k)\] For every $k \in {\mathbb{N}}$ there exists $\alpha(k) \geq 0$ such that $$\delta^k ( nset $$\delta^{k}(n) = R_{n-l}^{-1} \circ \dots \circ R_{n-2}^{-1} \circ
                                            R_{n-1}^{-1} ( \delta^n(n) ).$$ It remains to check that the numbers $\delta^k(n)$ have the desired properties.\
\[lem:choice-of-delta-n-k:increasing\] This follows since $R_L(\delta) \geq \delta$ and $R_L^{-1}(\delta) \leq \delta$.\
\[lem:choice-of-delta-n-k:alpha(k)\] For $n \geq k$ we have $\delta^k(n) \leq \delta^n (n) = \frac{1}{C(n)}$ by \[lem:choice-of-delta-n-k:increasing\]. Now we can choose $\alpha (k)$ to be $\max \{ 1 , C(n) \delta^k (1), \dots , C(n) \delta^k(k-1) \}$.\
\[lem:choice-of-delta-n-k:d\_and\_delta\] Since $R_k( R^{-1}_k (\delta) ) \leq \delta$ we conclude $$R_k ( \delta^k(n) )  \leq \delta^{k+1} (n).$$


          {{\times}}(\kappa + l,\infty) \\
\mbox{but} &
(h_{k_l / (k_l+t'_l)}(p(y'_l),e'_l),t'_l+k_l) &
                         \not\in & U {{\times}}(\kappa,\infty).
\end{array}$$ The metric control with respect to $\widetilde{d}^\infty_n$ for the morphism $\varphi$ implies that $p(y_l) = p(y'_l)$ for all sufficiently large $l$. From the metric control condition with respect to the projection to $[1,\infty)$, see , we conclude that $|t_l - t'_l| \leq \alpha$ for some $\alpha > 0$ independent of $l$. Since $t_l + k_l> \kappa + l$ this implies $t'_l + k_l> \kappa$ for sufficiently large $l$. Therefore we may assume that $$\label{equ:first-claim-not-in-U}
h_{k_l / (k_l+t'_l)}(p(y'_l),e'_l)
      Introduction {#s1}
============

Flooding events caused by heavy rainfall have increased globally over the past six decades as a consequence of climate change (Bailey-Serres et al., [@B4]). Flooding severely affects the productivity of farmland, because most agriculturally important crops are intolerant to flooding stress (Setter and Waters, [@B61]). One of the main effects of flooding is a marked reduction in oxygen availability due to the slower diffusion of gas in water as compared to air (Dat et al., [@B13]). Thus, when plants are exposed to flooding conditions, the roots initially suffer from oxygen deficiency (Sauter, [@B58]), leading to the inhibition of root respiration and a marked decrease in the energy status of root cells (Ashraf, [@B2]). In response to flooding, plants activate an alternative fermentation metabolic pathway to produce ATP


and regenerate NAD^+^ (Gibbs and Greenway, [@B20]), as evidenced by the drastic increase in alcohol dehydrogenase activity in soybean under flooding stress (Komatsu et al., [@B34]). Although the flooding-induced changes in energy metabolism are important processes in adaptation to flooding conditions, metabolism induced by flooding and adaption to flooding in soybean remain unclear.

Calcium is an essential plant nutrient that determines the structure of cell wall and membranes, and plays a role in regulation of growth and development (Hepler, [@B25]). Calcium also has functions in protecting the integrity of cell membranes, reducing membrane permeability, and preventing ion leakage caused by biotic and abiotic stresses (Lin et al., [@B38]). Exogenous calcium alleviated the suppression of plant growth and kept plant to maintain and modulate cellular function by relieving gene repressionspectroscopically at 600 nm.

Protein extraction
------------------

A portion (0.5 g) of fresh roots was ground to a powder in liquid nitrogen with a mortar and pestle. The powder was added to an acetone solution containing 10% trichloroacetic acid and 0.07% 2-mercaptoethanol, and the resulting mixture was vortexed and then sonicated for 10 min. The suspension was incubated for 1 h at −20°C with vortexing every 15 min, and was then centrifuged at 9000× g for 20 min at 4°C. The resulting supernatant was discarded and the obtained pellet was washed twice with 0.07% 2-mercaptoethanol in acetone. The pellet was dried using a Speed-Vac concentrator (Savant Instruments, Hicksville, NY, USA) and resuspended in lysis buffer containing 7 M urea, 2 M thiourea, 5% CHAPS, and 2 mM tributylphosphine by vortexing for


1 h at 25°C. The suspension was centrifuged at 20,000× g for 20 min at 25°C, and the supernatant was collected as protein extract. Protein concentrations were determined using the Bradford method (Bradford, [@B8]) with bovine serum albumin as the standard.

Protein purification and digestion for mass spectrometry analysis
-----------------------------------------------------------------

Extracted proteins (100 μg) were purified with methanol and chloroform to remove any detergent from the sample solutions, as previously described (Nanjo et al., [@B47]). Briefly, 400 μL methanol was added to 100 μL samples and then mixed. A total of 100 μL chloroform and 300 μL water were added to resulting mixture, mixed, and centrifuged at 20,000× g for 10 min to achieve phase separation. The upper phase was discarded, 300 μL methanol was added to lower phase, and theThe peptides were eluted from the trap column with a linear acetonitrile gradient (8--30% in 120 min) in 0.1% formic acid at a flow rate of 200 nL/min, and then loaded onto a C18 NANO HPLC NTTC-360/75-3 capillary tip column (75 μm ID × 120 mm, Nikkyo Technos) using a spray voltage of 1.5 kV. A nanospray LTQ Orbitrap mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) was operated in data-dependent acquisition mode with the installed Xcalibur software (version 2.0.7, Thermo Fisher Scientific). Full-scan mass spectra were acquired in the Orbitrap MS over 400--1500 m/z with a resolution of 30,000. A lock mass function was used for high mass accuracy (Olsen et al., [@B51]). The 10 most intense precursor ions were selected for collision-induced fragmentation in the


by 1 day flooding with calcium; however, there was no significant between 2 days flooding without and with calcium. The mRNA expression level of copper chaperone was similar to their protein level in the root and hypocotyl under 2 days flooding with calcium. In roots and hypocotyls, the level of copper chaperone was down-regulated by 1 day flooding without and with calcium; whereas the level was significantly up-regulated by 2 days flooding with calcium. In cotyledons, the mRNA levels of urease and copper chaperone were significantly up-regulated by 1 and 2 day flooding when calcium was exogenously added (Figure [5](#F5){ref-type="fig"}).

![**Effects of calcium on the mRNA expression levels of urease and copper chaperone proteins in different organs of soybean under flooding stress**. Two-day-old soybeans were flooded without or withof record.
We find no reason to hold an annexation petition invalid merely because it is signed by a person not contemplated by the statute, so long as the validity of the petition is not dependent upon that signature. Appellants do not contend that absent this signature the *42 petition would lack the requisite number of signatures. No authority nor good reason to the contrary has been suggested to this court.
[2] Appellants admit that the affidavit of Marjorie Elting is not defective on its face. However, they contend that her testimony at the hearing to determine the petition's validity when called as an adverse witness by the objectors under Section 60 of the Civil Practice Act, disclosed that her affidavit was not made upon her personal knowledge but upon


by Section 7-4, to constitute the petition prima facie evidence of such facts and require the objectors to overcome the prima facie case so made. Kryznowski v. Benoit, 30 Ill. App.2d 391, 174 NE2d 710.
Appellants-objectors do not contend, nor have they prevailed in their duty to prove, that the matters sworn to in the affidavit are not true.
In matters of annexation, where the necessary signatures may well run into the hundreds to present a valid petition, common sense dictates that the affidavit verified by Mrs. Elting should suffice.
[7] Turning now to the appellant-objector Chicago Title and Trust Company's assignment of errors, the objections relate to the objections set out in Section 7-3, except they do not allege ownership of perimeter lands.
The objections were filed on September 15, 1960,the USA, the land that destroyed her city and her fiancé, to live again with Elizabeth and with Harry’s daughter Kim. Hiroko and Kim find themselves embroiled in Raza’s plot to help his childhood friend escape back to Pakistan That’s a very abbreviated version of a complex and compelling plot and a short answer to that question of “How did it come to this?” without hopefully revealing any of the key plot twists.

Kamila Shamsie is one of my favourite authors and I’ve read several of her other books but none made such a mark on me as Burnt Shadows. She’s unafraid to take on the most shocking events of the 20th and early 21st century. From the atomic bombs dropped on Japan through to the attacks on the


“We came, we saw, he died!”

The U.S. during Barack Obama’s Presidency overthrew Ukraine’s democratically elected President Viktor Yanukovych, claiming that he was corrupt (actually the U.S. had long been trying to overthrow Ukraine’s democracy, even before Yanukovych came into power there), though all post-Soviet-era Ukrainian Presidents have been corrupt (the U.S. complaint was just an excuse to overthrow him); and America installed there to replace Yanukovych a regime controlled by anti-Russian racist fascists who promptly pushed for Ukraine to join NATO, the anti-Russia club that under American leadership has been expanding right up to Russia’s very borders even though the deal with Mikhail Gorbachev at the end of the Soviet Union and its Warsaw Pact, had been that if the Berlin Wall were to fall, then NATO wouldat the time when the Soviet Union broke up, and its former member-states headed to become democracies, the United States (if not also most of its NATO allies) headed towards dictatorship, and is now solidly into it. (Bernie Sanders in the current U.S. Presidential primaries is hoping to reverse that trend and to restore the FDR-progressive pro-democracy American tradition, but that’s considered a longshot effort in today’s fascist America.)

When Obama in private told the assembled Wall Street CEOs assembled inside the White House on 27 March 2009 “My administration is the only thing between you and the pitchforks,” he was referring to the Wall Street chieftains whom, and whose investors, his Administration was bailing out with taxpayer dollars, as being like the Blacks that the KKK had infamously


the American Revolution.

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many of these animals out here.

I know how you feel I don't like cyclists either. ;) It should be against the law for anyone other than an 18-21 yr. old girl to wear spandex.

CLibertarian

06-27-2008, 03:33 PM

I know how you feel I don't like cyclists either. ;) It should be against the law for anyone other than an 18-21 yr. old girl to wear spandex.

In my mountain biking days, I wore the lycra shorts because, they had padding and wicked the sweat away. However, Since I thought that it should be a violation of some local decency ordinance to do so:D, I wore some loose shorts over them.

Odysseus

06-27-2008, 06:02 PM

I know how you feel I don't like cyclists either. ;) It should be against the law for anyone otherthat you can experience it with us. The power was out, candles were lit and I liked the mood so I went with it! By the time the power came back on we already started and to fire up all the lights, especially in that heat would have made it just another shoot; this one happened as it happened and we rolled. Everything just created a very erotic interesting mood in the room and the shoot was a success in spite of the curve balls m other nature threw us again. A great film, real, raw, unscripted and shot as it happened where it happened, #98, done in true Muscle Mafia style was a success and one of the favorites to shoot for all of us.

The Sounds and


throated Brad�s 9x7 monster cock and had his tongue all over Brad�s balls and up Brad�s ass. When Brad was ready to feed Keith his load, he wanted you all to see his cum shot, so he busted off into his own hand. With a palm full of thick muscle cum Brad made Keith eat it right out of his hand.

Note: Later that night, Matt came to the shoot location. He and Brad did some shots together and both took advantage of the erotic atmosphere created with the fans and candles. A second film was shot this hot, sticky and stormy summer day and will be a future release. If opt�d into our mailing list you will be notified of that release and all the others set forsuch as Graf et al. reported that target volumes can be reduced overall by about 10% \[[@CR8], [@CR11]\]. Additional precision of added ^68^Ga-DOTATOC-PET has been reported by using PET-MRI combination devices minimizing any positioning or matching errors \[[@CR9], [@CR12], [@CR14]\].

Thus, the contribution to target volume reduction has been shown. However, it is unclear whether this reduction, does actually result in a meaningful and clinically reduction of dose to OAR. Moreover, it is unclear of this modification of target volumes is independent of the radiation technique applied.

Therefore, in the present work we determined the impact of target volume modification by use of ^68^Ga-DOTATOC-PET for RT planning in patients with skull base meningiomas. We calculated the potential of dose reduction for different high-precision techniques comparing advanced photons to protons.

Methods {#Sec2}
=======

Patient characteristics


definition in radiation oncology significant reduction of target volumes compared to CT and MRI only can be achieved. The present manuscript describes the potential to reduce dose to OAR by adding PET to treatment planning. Modification of target volumes reduces dose to OAR with photon radiotherapy. The effect is highest when OAR are not included in the target volumes. For the skull base tumors evaluated, the greatest benefit is seen for the brain stem as well as the optic chiasm. For patients treated with protons, the PET-effect is minimal, mainly because dose conformality and dose outside the target is already reduced due to the physical properties of particle beams.

To optimize the therapeutic window in radiation oncology minimizing dose to normal tissue is an essential aim. Modern RT techniques,Mostly lesions extending into soft tissue e.g. parapharyngeal meningiomas, seem larger on MRI than the real volume as shown on PET; for bony meningiomas, which are often difficult to identify by MRI and often only visibly on CT imaging in bone windows target volumes are enlarged with PET-target volume definition. However, to date, no prospective trials comparing target volume definition based on MRI versus PET have been performed, and most data on radiotherapy for meningiomas derives from MRI-based treatment planning. Therefore, one must keep in mind that modifications in target volumes might potentially convey to changes in clinical outcome.

The potential of PET-planning to reduce treatment volumes and thus potentially increase dose to the target while reducing dose to OAR has been evaluated only in few trials. One planning


idea of integral dose reduction is strongly associated with proton therapy \[[@CR20]\]. Due to the physical properties of protons sparing of normal tissue outside the target volumes is possible. Several groups have calculated an alleged risk reduction for secondary cancers, neurocognitive decline or other side effects \[[@CR21]--[@CR25]\]. However, to date, no prospective studies have confirmed this clinical hypothesis.

Conclusions {#Sec11}
===========

In conclusion, the potential to further reduce dose to OAR with protons is minimal. However, with IMRT, the benefit is significant for all OAR evaluated. Therefore, with advanced treatment planning improvement of high-end IMRT is achieved moving treatment plans closer to those achieved by particle therapy. Therefore, comparing of advanced photons with high-end imaging for treatment planning to proton therapy potentially leads to comparable results, in terms of tumor controland UH reviewed the data. All authors discussed the data. SEC drafted the manuscript. All co-authors read and approved the manuscript.

Ethics approval and consent to participate {#FPar3}
==========================================

The study was approved by the Ethic's Committee of the Medical Faculty, University of Heidelberg.

Consent for publication {#FPar4}
=======================

Not applicable

Competing interests {#FPar5}
===================

The authors declare that they have no competing interests.

Publisher's Note {#FPar6}
================

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.