a progressive opening of the grip with straightening of the fingers, followed by a closure of the grip until it matches object size. The point in time where grip size is the largest (maximum grip size) is a clearly identifiable landmark that occurs well before the fingers come into contact with the object.

In neural terms, grasping behavior can be dissociated into separate reach and grip components (for review, see [@pone.0065508-Castiello1]--[@pone.0065508-Grafton1]). According to this view, computations regarding the grasp component occurs within a lateral parietofrontal circuit involving the anterior intraparietal area (AIP) and both the dorsal (PMd) and the ventral (PMv) premotor areas [@pone.0065508-Godschalk1]--[@pone.0065508-Weinrich1]. The general agreement is that the processes occurring in AIP constitute the initial step of the transformation leading from representation of objects to movement aimedcorresponding to the time at which the object was approached and contact points have to be optimized.

![Grand-average ERP waveforms and amplitude scalp map of the P300 component.\
The plots in panel A show ERPs time-locked to goggles opening at parietal electrode sites in Grasping Large (blue line) and Grasping Small (red line). In panel B, the topographical distribution of the P300 component is represented. Differences between the two experimental conditions (Grasping Large-- Grasping Small) were considered.](pone.0065508.g003){#pone-0065508-g003}

![Grand-average ERP waveforms and difference amplitude scalp map of the m-N400 component.\
The plots in panel A show the ERPs time-locked to goggles opening at frontal, centro-frontal and central electrode sites for the Grasping Large (blue line) and the Grasping Small (red line) conditions. In panel B, the topographical distribution for the m-N400 component is


{#s3c4}

Mean ERP amplitude extracted in this time window at frontal, fronto-central, central, and parietal electrode sites was analyzed. The ANOVA confirmed that higher ERP amplitude was found for the GS condition \[main effect of stimulus size: F(1,21) = 28.08, p\<0.001; η^2^ ~p~ = 0.57\]. A significant main effect of anterior-posterior electrode position \[F(2,20) = 10.28, p\<0.001; η^2^ ~p~ = 0.33\] showed that ERP in such time window were larger at frontal, fronto-central and central sites compared to parietal positions (all ps\<0.030). A significant main effect of left-right electrode position \[F(2,20) = 12.47, p\<0.001; η^2^ ~p~ = 0.37\] revealed that mean ERP amplitude within the 1200--2000 ms time-window was maximal at midline compared to both left and right sites (all ps\<0.002).

Correlations between Kinematic and ERP Events {#s3d}
---------------------------------------------

Mean amplitude and latency of P300 and m-N400 components were averaged at electrode sites inwhich they were maximally expressed. Specifically, at P3 and Pz for the P300 and at Fz and FCz for the m-N400. Then these values were correlated with movement duration and the considered kinematic measures, namely time to peak velocity and the time of maximum grip aperture. No significant correlations were detected when considering the relationship between kinematical and ERP events. However, as depicted in [Figure 5](#pone-0065508-g005){ref-type="fig"}, for both the GL and the GS experimental conditions, the individual mean latency for the m-N400 component significantly correlated with the individual mean for movement time \[for GL: r(22) = 0.49, p = 0.022; for GS: r(22) = .46, p = 0.034\].

![Correlation between movement time and ERP measures.\
Correlation between the individual data for movement time and the m-N400 latency for the Grasping Large and the Grasping Small conditions.](pone.0065508.g005){#pone-0065508-g005}

Discussion {#s4}
==========

We set


et al. [@pone.0065508-Berthier1] also showed that as visual information and object size decreased, subjects had longer movement times, slower speeds, and more asymmetrical hand-speed profiles. This kinematic characterization reflects the adoption of a strategy following the principles of the Fitt's Law [@pone.0065508-Fitt1], implying that the difficulty of the task is reflected in movement kinematics. Thirdly, in macaques, it has been revealed that of the premotor area F5 neurons active during grasping, the most frequent were those involved in precision grips [@pone.0065508-Rizzolatti1] whereas whole hand neurons were encountered much less frequently [@pone.0065508-Rizzolatti1], [@pone.0065508-Jeannerod3]. Finally, neuroimaging studies indicate that premotor activity increase more during the execution of a movement toward a small object than toward a large one reflecting the increased planning and on-line control required by grasping small objectsFor this reason, it distressed heavily even though I used a fine grit sand paper & a light touch, because the paper caught on all the imperfections. Also, the color turned from white to cream, almost yellow actually; I had envisioned white. I had purchased pewter hardware for it that did not look good. I wasn’t satisfied at all. I set it aside and looked at it for the next two days. Finally, inspiration hit: The table reminded me of a stack of beautiful Antique letters I’d come across in a trunk while out antiquing!!! I wish I had taken a photo of them, but google images will give you the gist. The coloring of the table is a perfect match to the antique white look of the


camera pans out from the spot they’re sat at, covered in daisies, to reveal they’re in a graveyard filled with hundreds of seemingly identical white gravestones symbolising the transition Harold is making from being obsessed with death to slowly appreciating life. It’s a very touching moment and one which has always stood out to me. (To see a clip of this scene, click here. You really ought to watch the whole film though!)

Whilst both the acting and plot itself are of a very high quality, however, so is Ashby’s directing.

Harold and Maude is an exceptionally quirky film and one which could have easily descended into unnecessary madness. Instead, Ashby manages to maintain the charming eccentric nature of the film whilst providing us with one of the greatest loveGlucagon-like peptide-1 regulates the beta cell transcription factor, PDX-1, in insulinoma cells.
Glucagon-like peptide-1 (GLP-1) enhances insulin biosynthesis and secretion as well as transcription of the insulin, GLUT2 and glucokinase genes. The latter are also regulated by the PDX-1 homeoprotein. We investigated the possibility that GLP-1 may be having its long-term pleiotropic effects through a hitherto unknown regulation of PDX-1. We found that PDX-1 mRNA level was significantly increased (p<0.01) after 2 hours and insulin mRNA level was subsequently increased (p<0.01) after 3 hours of treatment with GLP-1 (10 nM) in RIN 1046-38 insulinoma cells. Under these experimental conditions, there was also a 1.6-fold increase in the expression of PDX-1 protein in whole cell and nuclear extracts. Overexpression of PDX-1 in these cells confirmed the finding of the wild


y) = \rho_1\frac{\sigma_x}{\sigma_1}y \cdot p_1 + \rho_2\frac{\sigma_x}{\sigma_2}y \cdot p_2 = \mu_{x \mid y},$$
where $p_i = \mathbb{P}(Y = Y_i \mid Y = y)$.
Returning to the conditional variance formula, we now expand it, conditioning on $Y = Y_i$ again:
$$\mathbb{V}(X \mid Y = y) = \mathbb{E}((X - \mu_{x\mid y})^{2}\mid Y = y)$$
$$= \mathbb{E}((X - \mu_{x\mid y})^{2}\mid Y = y, Y = Y_1)\mathbb{P}(Y = Y_1 \mid Y = y) + \mathbb{E}((X - \mu_{x\mid y})^{2}\mid Y = y, Y = Y_2)\mathbb{P}(Y = Y_2 \mid Y = y).$$
Now $$(X - \mu_{x\mid y})^{2} = (X - \mu_{x \mid y,i} + \mu_{x \mid y,i} - \mu_{x\mid y})^{2} = (X - \mu_{x \mid y,i})^2 + 2(X - \mu_{x \mid y,i})(\mu_{x \mid y,i} - \mu_{x\mid y}) + (\mu_{x \mid y,i} - \mu_{x\mid y})^2,$$
where $\mu_{x \mid y,i} = \mathbb{E}(XMindia Gavasheli, said, “Any assertion that we are not a news organization is simply false.” He also said he was unaware of the FBI probe. “This is the first time I’m hearing about it, and I don’t think anyone at Sputnik was contacted, so thank you for letting us know,” Gavasheli said.

Gavasheli attributed the push to have Sputnik register through FARA to paranoia surrounding Russia. “I think it tells about the atmosphere of hysteria that we are witnessing now,” Gavasheli said. “Anything being related to Russia right now is being considered a spycraft of some sort.”

Story continues


take the medicine, check
with your pharmacist first
because certain foods,
like yogurt or applesauce, can
stop the medication from working.
And others, like honey or
syrup, work only if your
child is not on a restricted diet.
If you do use food or drink,
use a very small amount,
like this, so that the
child will finish it all.
When giving medicine to any
child, it's important that you
double check the amount and
the dose with the instructions
that are written on the bottle.
If you use a log or a medicine
diary, that can help to prevent
either giving too many
doses or missing some doses.
for many Finds, because the set that is searched is very large. It could however run concurrently to split the search up to multiple threads. For example with two threads: The first thread searches the first half, the second thread searches the second half.
The futures are not really guaranteed to be valid, because the user has to call the Search function. Would it be possible to start the search, once the first .get was called?
My "algorithm" to fulfil the promises looks very ugly and complicated, but I can't think of a better way.

A:

There are definitely some problems if you want to make this threaded. Firstly, access to m_searches is not protected in any way. Since this is a shared bit of memory, it would need to be protected


$s^d$ original spins) do not have a fixed modulus – simply, the original model does not include the constraint $\mid \varphi({\bf x}) \mid = const$ for the block averages. It means that the transformation with any finite $s$ yields a Hamiltonian of form different from the original one, i. e., the original Hamiltonian with merely renormalized coupling constant can never be the fixed–point Hamiltonian. This is the common problem in the perturbative RG theory that the renormalized Hamiltonian is assumed (or even “proved” like in [@BZ]) to have the same form as the original one. This, however, appears to be wrong in view of the exact renormalization.

In absence of rigorous proofs, theoretical predictions can be supported by appropriate empirical data. However, the experimental measurements of susceptibility $\chi$ depending on fieldManchester United are closing in on two major signings with deals for the Borussia Dortmund attacking midfielder Henrikh Mkhitaryan and Zlatan Ibrahimovic looking likely after a busy Saturday.

José Mourinho is keen to sign both players as he steps up his recruitment drive before his first season in charge at Old Trafford. Mkhitaryan has one year left on his contract at the Bundesliga club and, while Dortmund have publicly said that he is not for sale, they have now admitted that the player is ready to move on.

Euro 2016: 10 transfer targets whose stock has risen sky-high in France Read more

United are ready to bid £30m for the 27-year-old and are confident that Dortmund will accept their offer. The Armenian, who joined Dortmund in 2013 from Shakhtar Donetsk for


cell lines to chemo- or radiotherapy.

Colorectal cancer is the third leading cause of cancer-related mortality in the United States (US). The estimated five-year survival rates range from approximately 90% for patients with stage I disease to less than 5% for patients with stage IV disease. The causes of CRC are complex and involve the interaction between genetic and environmental factors. The vast majority of CRC cases can be attributed to environmental causes, as they account for more than 70% of all incidences \[[@B36-molecules-23-03309]\]. Some aspects of western dietary patterns may also influence the risk of CRC. Adiposity and higher levels of insulin in obese subjects are connected with an increased risk of CRC \[[@B37-molecules-23-03309]\]. Diets rich in animal products and processed grains have been associated with higher C-peptidecancer.

Coffee consumption was found to be significantly associated with a reduced risk of CRC in Japanese people \[[@B65-molecules-23-03309]\]. The risk of colorectal, colon, and distal colon cancer decreased with more frequent consumption of coffee, up to 1--3 cups per day, but increased in people who drank more than 4 cups per day. There was no association noted for proximal colon cancer or rectal cancer. According to the results of another population-based prospective cohort study of Japanese men and women, the consumption of coffee was not associated with CRC risk for both men and women, but reduced the risk of invasive colon cancer in women ingesting less than 1, 1--2, and more than 3 cups of coffee per day (relative risk (RR) 0.79 vs. 0.74 vs. 0.44, respectively, *p*-trend


than 5 cups of coffee per day \[[@B86-molecules-23-03309]\]. The consumption of 4 cups per day reduced the risk of CRC by 7%, whereas the inverse association was stronger at higher levels of coffee intake. A meta-analysis performed within the Continuous Update Project, analyzing research on cancer prevention and survival, performed by Norat et al., found no significant associations between coffee consumption and the risk of colorectal, colon, and rectal cancer in pooled and stratified groups \[[@B87-molecules-23-03309]\].

There has been no clear association between coffee consumption and the risk of colorectal and subsite-related cancers in case--control studies, while prospective cohort studies have been rather unambiguous in that respect and have shown no relationship. Interestingly, almost all inverse and only a few positive associations of CRC risk and coffee consumption havegroup of plant polyphenols widely present in the human diet: Fruits, vegetables, coffee, tea, wine, and propolis \[[@B142-molecules-23-03309],[@B143-molecules-23-03309]\]. Caffeoylquinic acid may theoretically have four isomers, but only three are present in plants and contain chlorogenic acid: 3-*O*-caffeoylquinic acid (3-CQA), neochlorogenic acid (5-*O*-caffeoylquinic acid, 5-CQA), or cryptochlorogenic acid (4-*O*-caffeoylquinic acid, 4-CQA). The most popular isomer, 5-CQA, is called chlorogenic acid (PubChem CID: 1794427CGA, CGA). It is an ester composed of caffeic acid and (−)-quinic acid \[[@B144-molecules-23-03309]\]. The major source of chlorogenic acid in human diet is coffee, and its ingestion varies, depending on daily coffee consumption: Coffee drinkers ingest 0.5--1 g, while coffee abstainers ingest less than 100 mg of it per day \[[@B134-molecules-23-03309]\]. Olthof et al. determined that about one-third of chlorogenic acid is absorbed intestinally \[[@B127-molecules-23-03309]\], while


demonstrated the inactivation of phosphorylated ERK and increased levels of phosphorylated p53 in both lines treated with both concentrations of CGA. As the authors stated, S-phase arrest and inactivation of ERK may explain the lower viability of CGA-treated cells. Inhibition of the proliferation of the Caco-2 cell line was also found in a study performed by Wang et al. \[[@B165-molecules-23-03309]\]. Wang et al. found an inhibition of proliferation in Caco-2 and HepG2 cells in vitro after administration of chlorogenic acid, with a median effective dose of (EC~50~) 2.8 ± 0.7 μg and 2.3 ± 1.1 μg, respectively. Sato et al. demonstrated that CGA and CA demonstrated antioxidant activity in human colon cancer cell line Caco-2 in a dose-dependent manner \[[@B121-molecules-23-03309]\]. Antioxidant activity was measured using total antioxidant performanceprimarily on jobs. He promised not just to Make America Great Again, but to make it “really, legitimately, so great.”

But as always, politics—not policy specifics—preoccupied the candidate.

“We’re gonna end at a very high level and get a lot more delegates than anybody projected in their wildest imaginations,” he said.

“This has been an incredible evening. It’s been an incredible day and week!” he said. “It has really been something amazing.”

Like Clinton, he suggested it was time for primary season to come to an end.

“We don’t have much of a race anymore, based on what I’m seeing on television,” he added, “Sen. Cruz is just about mathematically eliminated—and we’ve won another state.”

Ted Cruz was beaten into third place in the New York primary with just 14.5 percent of the vote,


Commission on Non-Ionizing Radio Protection (ICNIRP) \[[@B6-ijerph-11-09460],[@B7-ijerph-11-09460]\] or the International Commission on Electromagnetic Safety (ICES) \[[@B8-ijerph-11-09460]\]. These guidelines protect people from known health effects with a substantial safety margin (often 50 for the public and 10 for occupationally exposed people). The fundamental limits (called basic restrictions) refer to the biological effects induced by incident electromagnetic fields. In the RF range, the relevant quantity of the basic restriction is the SAR (see next section). In the low frequency and intermediate frequency domain the induced electric field strength in human tissues is quantified. In the terahertz range power density is defined as the basic quantity. In 1999, the EU established a common protective framework with a recommendation on the limitation of the exposure of the general public to electromagnetic fieldsslots and repeated 3--4 times. A time slot may cover 10--30 min. The whole campaign can be scheduled once or repeated several times, e.g., every year, depending on budgetary and statistical requirements.

This monitoring approach allows, first, to identify typical (not: statistically representative) exposure levels in interested compartments, second, to record overall and compartment specific exposure trends, third to construct personal exposure profiles based on lifestyles. A lifestyle can be defined with the help of the number of times a person spends in specific compartments. Personal exposure can then roughly be assessed for such ideal lifestyles. The approach can also be combined with exposure modelling \[[@B40-ijerph-11-09460],[@B52-ijerph-11-09460]\], although exposure from very small installations that do not need an authorization (e.g., femto cells) can only be included in the measurements but


to four months. Depending on budgetary and statistical requirements, the sample size could be increased or the measurements could be repeated.

Such data does not allow generalizations to the population at large. Nevertheless, it informs about typical exposure levels and patterns, about exposure differences between lifestyle groups, and about exposure trends. Yet, interpretation of the data is challenging. A diary that logs the activities of the subjects and links this information with the exposure data strongly supports data analysis and interpretation. It allows, for instance, to identify and compare exposures in/between different compartments (microenvironments)---indoor, outdoor, on the move---and for different activities. The latter is especially important for assessing the contribution of exposure from devices used close to the body to overall personal exposure. However, it has to be noteddose.

In order to estimate real life doses \[[@B28-ijerph-11-09460]\], source modelling and measurement data will be combined with detailed digital human models \[[@B75-ijerph-11-09460]\], derived from MRI-scans. In this way not only whole-body doses, but also organ and tissue specific local doses (e.g., mobile phone radiation, exposures by electric household appliances) \[[@B26-ijerph-11-09460],[@B28-ijerph-11-09460],[@B76-ijerph-11-09460],[@B77-ijerph-11-09460],[@B78-ijerph-11-09460]\] can be estimated. Depending on the health outcome of interest, different organ or tissue exposures may be relevant. However, this dose modelling is faced with a series of challenges that have not yet been sufficiently investigated and where only limited experiences exist \[[@B79-ijerph-11-09460]\]: Near-field (close-to-body) sources: exposure from portable consumer goods (mobile phones, DECT phones, Bluetooth and WiFi equipment, to list but devices from the RF domain) represent a major, or even---especially for young people being heavy users


remaining thinking people in America came to distrust the mainstream. The initials “MSM” standing for “mainstream media” became an expression of derision and contempt, not undeserved given the MSM’s repeated failure to fight for the truth.

National Democrats, too, showed little fight. When evidence of Republican misconduct was available as in the investigations of the early 1990s into Iran-Contra, Iraq-gate and the October Surprise case accommodating Democrats, such as Rep. Lee Hamilton and Sen. David Boren chose to look the other way. [See Robert Parry’s America’s Stolen Narrative.]

The Democrats even submitted when the Right and the Republicans overturned the electoral will of the American people, as happened in Election 2000 when George W. Bush stole the Florida election and thus the White House from Al Gore. [For details, seeoverheating planet; costly foreign wars; a bloated Pentagon budget; and children massacred by troubled young men with ridiculously easy access to semi-automatic assault rifles.

Yet, if rational and pragmatic solutions are ever going to be applied to these problems, it is not just going to require that President Obama display more spine. The country is going to need its conscious inhabitants of the real world to stand up with at least the same determination as the deluded denizens of the made-up world.

Of course, this fight will be nasty and unpleasant. It will require resources, patience and toughness. But there is no other answer. Reality must be recovered and protected if the planet and the children are to be saved.

Investigative reporter Robert Parry broke many of the Iran-Contra stories for


The Associated Press and Newsweek in the 1980s. You can buy his new book, America’s Stolen Narrative, either in print here or as an e-book (from Amazon and barnesandnoble.com).

Parry Book Deals Coming to an End

From Journalist Robert Parry: The holiday specials for buying my books free postage and a second book for a nickel are coming to an end on Jan. 20. So, now is a great time to buy my new book, “America’s Stolen Narrative.”

If you buy “America’s Stolen Narrative” for $24.95, you will get free shipping and handling. Plus, for another nickel, you can get a companion book, either “Secrecy & Privilege: Rise of the Bush Dynasty” or “Neck Deep: The Disastrous Presidency of George W. Bush,” which I co-wrote with two of my sons, Samhave made a donation and would like a signed gift copy of my new book, America’s Stolen Narrative, as a token of our appreciation, just ask.

Thank you again for your support.

Robert Parry

Robert Parry broke many of the Iran-Contra stories in the 1980s for the Associated Press and Newsweek. He founded Consortiumnews.com in 1995 as the Internet’s first investigative magazine. He saw it as a way to combine modern technology and old-fashioned journalism to counter the increasing triviality of the mainstream U.S. news media.


of the stars are lower than the predicted values.

The fluorine abundance patterns in the low metallicity regime were also explored by [@Cunha2003], who derived F abundances for a sample of giants in the Large Magellanic Cloud and in the globular cluster $\omega$ Cen. They found that the abundance ratio of F/O declines as the oxygen abundance decreases. They therefore suggested that the observed low values of F/O exclude AGB synthesis as the dominant source of fluorine in their targeted stellar populations. In particular, the targeted stars in $\omega$ Cen did not have enhanced F abundances, despite their large s-process abundance values. In addition, they claimed a qualitative agreement with what is expected if $^{19}$F is produced via H- and He-burning sequences in very massive stars (fluorine being ejectedYour donations help us deliver My Stuff Bags to waiting children. Please donate now or call the Foundation at 866.3MY.STUFF.

Why it Matters

While we do our best to describe how important receiving a My Stuff Bag filled with new belongings is to a frightened child rescued from abuse, neglect, homelessness or abandonment, the tremendous emotional impact of these duffels is best conveyed in the heartfelt words of agency staffers, and of course, the children themselves. It’s also important to know why the My Stuff Bags program matters to the many generous people who support it. Click on the links at right to hear it directly from them.


it is up to you to decide on how honest to be.
What an immigration lawyer told me (a neighbor) is that if your qualifications are solid for the visa, having applied for the lottery has little effect.  But if you are borderline, then it becomes a bigger factor.

The invention relates to a digital transmission system comprising a transmitter and a receiver, for transmitting a wide-band digital signal having sample frequency F.sub.s, for example a digital audio signal, via a transmission medium, and for receiving said signal; and more particularly to such a system which transmits a second digital signal which comprises consecutive frames, and each frame comprising a plurality of information packets, each information packet comprising N bits, N being larger than 1. The receiver comprises a decoder having an input for receiving the second digital signal, and a decoder has an output coupled to an output terminal to supply the wide-band digital signal.
The invention also relates to a transmitter and a receiver for use in the transmission system, to a transmitter in the form


"rotate" prop down to styled component, so I did a little refactoring on your code and added a container component to hold rotation state:
https://codesandbox.io/s/wyx6pqj13w
Hope this helps!
Code in the link is shown here
 import React, { Component } from "react";
 import ReactDOM from "react-dom";
 import styled from "styled-components";

 import "./styles.css";

 const IconButton = () => <button>Icon</button>;

 const IconButtonWrapper = styled.div`
   float: right;
   transform: rotate(0deg);
   overflow: hidden;
   transition: all 0.3s ease-out;
   transform: ${props => (props.rotate ? `rotate(180deg)` : "")};
 `;

 class IconButtonContainer extends Component {
   state = {
     rotate: false
   };

   handleClick = () =>
     this.setState(
       prevState => ({Q:

"Lives" vs. "life" in "the life of those living on the farm"

James is giving a tour of his farm to some of his friends. Which sentence is correct:

James introduces some of the animals on the farm: "This is Elmer, the pig... That's Mini, the mouse, and that duck is called Daffy... "
James tells his friends about the life of those living on the farm. His friends follow each word.

or

James introduces some of the animals on the farm: "This is Elmer, the pig... That's Mini, the mouse, and that duck is called Daffy... "
James tells his friends about the lives of those living on the farm. His friends follow each word.

I understand that the singular of life is more appropriate when discussing a shared life, but this distinction is


Q:

Charge payment "to", "on", or "against" a credit card/an account; charge a credit card/account/a person "with"an amount

Are all of these options acceptable and in current use to denote payment with a credit card?
E.g.
Please charge this amount on me/my credit card/my (credit card) account.
Please charge this amount to me/my credit card/my (credit card) account.
Please charge this amount against my credit card/my credit card details (or information).
Please charge me/my credit card/my (credit card) account with this amount.
And also:
"Thank you for providing us (with) your credit card and for authorizing us...
to charge it with a deposit;
Or
to charge a deposit (to it);
Or
to charge a deposit (on it);
Or
to charge a deposit (against it)."
Thank you.

A:

Please charge this amount on me/ (to/on) my credit card/my (credit card) account.
Please charge this amount to me/(to/on) my creditQ:

GAE Python: how get last modified date of static file

I'm adding some static files to my Python app on Google app engine. I do it like this, as described here.
app.yaml:
handlers:
- url: /stylesheets
  static_dir: stylesheets

I put my files in the subdirectory stylesheets within the my_project_app folder.
How can I get the last modified date of a file in stylesheets?

A:

Answer is simple make static files accessible that is all.
https://cloud.google.com/appengine/docs/python/config/appconfig#Python_app_yaml_Static_file_handlers

application_readable
Optional. By default, files declared in static file handlers are
  uploaded as static data and are only served to end users, they cannot
  be read by an application. If this field is set to true, the files are
  also uploaded as code data so your application can read them. Both
  uploads are charged against your code and static


that to start impeachment proceedings, a document or “resolution calling for a committee investigation of charges against the officer in question” must be introduced in the House of Representatives. Such a move would have been appropriate from the moment that Trump became president.

As documented in depth on the ImpeachDonaldTrumpNow.org website — where more than 600,000 people have already signed a petition for impeachment — the president continues to violate two “emoluments” clauses in the Constitution. One prohibits any gifts or benefits from foreign governments, and the other prohibits the same from the U.S. government or any U.S. state.

To uphold the bedrock principle that no one should be above the supreme law of the land, a resolute member of the House must now take the lead in introducing aAfter a couple of weeks of development, we are proud to announce the first version of Crypto Central is live! ✌

As product makers and crypto lovers, we searched for a nice UX/UI app, with core features as market prices, profit/loss indicator and market capitalization but we never had a crush on the perfect solution. So we build it 😄

Another portfolio? I already use a couple of apps!

Yeah, I know, me too! But do you really want your data sold to some companies you don’t they even exist? Did you never ask for tokens/features that were never built on the product? Not tired of the poor UX yet? 😫

It’s time to make the switch! 😻

Let’s go to the moon together!

Crypto Central app has been built to enhance your portfolio,


int row_size =
        min(n_boxes - row_start * threadsPerBlock, threadsPerBlock);
  const int col_size =
        min(n_boxes - col_start * threadsPerBlock, threadsPerBlock);

  __shared__ float block_boxes[threadsPerBlock * 5];
  if (threadIdx.x < col_size) {
    block_boxes[threadIdx.x * 5 + 0] =
        dev_boxes[(threadsPerBlock * col_start + threadIdx.x) * 5 + 0];
    block_boxes[threadIdx.x * 5 + 1] =
        dev_boxes[(threadsPerBlock * col_start + threadIdx.x) * 5 + 1];
    block_boxes[threadIdx.x * 5 + 2] =
        dev_boxes[(threadsPerBlock * col_start + threadIdx.x) * 5 + 2];
    block_boxes[threadIdx.x * 5 +resulting from this litigation," Gertner wrote in granting the internet coverage.

The 1st Circuit did not indicate when it would rule.

The RIAA also said the broadcast "creates a serious risk of unfairly infecting the pool from which the jury in this case will be selected."

The RIAA, which has sued about 30,000 individuals on allegations of copyright infringement, claims it is winding down its 5-year-old litigation campaign. The recording industry's litigation and lobbying arm told the circuit court that, "The public interest will not be served by broadcasting a single snippet of these proceedings, because doing so places a misleading emphasis on a limited aspect of the judicial process."

Image: Modernhumorist.com

See Also:


       }

        if ( Double.isNaN(d) )
            return "NaN" ;

        // Otherwise, SPARQL form always has "e0"
        String x = Double.toString(d) ;
        if ( (x.indexOf('e') != -1) || (x.indexOf('E') != -1) )
            return x ;
        // Renormalize?
        return x + "e0" ;
    }

    static public String stringForm(float f) {
    Q:

Items.Restrict Received Count not returning correct value

I'm writing a sub to return messages older than 12 hours (among other things.) This is a small excerpt.
Dim timeMinusTwelve as String
Dim itemsOverTwelve as Integer

timeMinusTwelve = Now - .5

itemsOverTwelve = Application.GetNamespace("MAPI").Folders("MyBox").Folders("Inbox").Items.Restrict("[Received] > '" & timeMinusTwelve & "'").Count

MsgBox itemsOverTwelve

" > " returns 0, " < " returns the total items in the folder. The real result is somewhere in the middle. Where is my syntax wrong?

A:

look at this enter link description here under the date header, you may need to modify the format of the date. May be Format(now()-0.5, "ddddd h:nn AMPM")


there's hope.
Like, it's gonna be okay it's like that.
I don't know what that sound
is, It's like spiritual, man.
This one is called alleviating stress,
I feel so relaxed.
I'm really happy that I've
prioritized meditation as a part
of my routine.
In the past, I was definitely
using it as just another task
that I'd cross off.
But I'm a lot more gentle on myself.
I've always worked well with
streaks because when I'm
working towards something, and
I've already built something,
it's easier for me to maintain.
When I like the past two
months, I was like, okay,
there's gonna be a day,
some point in this year
where I'm gonna miss a day

Spanish:
nosotros vamos a usar mi aplicacion favorita, calm.
Yo recientemente cambie mi cancion, escuchala.
No suena como si estas en un spa?
Cuando yo escuche esto, yo siento que hay esperanza.
Cual, va a estar bienusaré como una
cucharada.
Esto es como el aderezo que te gusta cuando vas a Benihana
y te dan como esta ensalada alado.
Este es el aderezo que va arriba de el.
Hay algo muy muy consolador sobre este sabor.
Eso se mira muy bien.

English:
I actually want something
relatively healthy
because I haven't been
eating the healthiest
the past couple of days.
So actually was the thing with
like nutrition and health.
So I'm gonna go and make myself a salad.
So for my protein, I'm
using a veggie patty.
This one is by Dr Preggers
and it's my favorite brand
of all the veggie patties.
So first off, we've got
some romaine lettuce,
Now I've got some cherry tomatoes,
I'm just gonna slice them in half.
Next up, we've got a cucumber.
This is a Japanese Musa
dressing and it is so delicious.
It's like on the creamier
side so ill just use


like a
spoonful of it.
This is like the dressing that
like when you go to Benihana
and they give you like that side salad.
This is the dressing
that goes on top of it.
There's something very very
comforting about this flavor.
That looks really good.

Chinese:
想吃點健康的
因為這幾天吃得都不是很健康ㄟ

希望能健康營養兩者兼顧
那就來做沙拉吧!
蛋白質的話就來點素食餅乾
阿刀博士是我最愛素食餅乾之一

首先先來點首先先來點蘿蔓生菜
櫻桃番茄
切一半
一根小黃瓜
加點日式千島醬簡直絕配
它裡面有點乳脂加一匙就好

你去紅花(美國的日式料理店)
它會擺在沙拉區旁邊
淋一點在上面就香氣四溢

超棒的

Spanish:
Y luego dejamos caer la pequeña empanada,
entonces voy a mostrarle a ustedes como he estado comiendo atentamente.
Es algo que he estado implementando por una semana
y media ahora y me a estado gustando hasta ahora.
Antes de que coma, yo me pregunte a mi misma una cuantas preguntas.
Entonces la primera pregunta es dos a tres emociones
que tu estas sintiendo, entonces ahorita yo me siento calmada,
me siento tranquila.
La segunda pregunta es porque tu quieres la comida.
Entonces esto tal vez es como una pregunra extraña,
pero te hace consciente decomo, porque estas comiendo?
Estas comiendo esto porque tienes hambre?
Ha pasado un tiempo desde tu última hora de comer?
Porque estas comiendo esto porque
anhelas algo dulce?
Estas comiendo esto porque estas aburrida?
Estas comiendo esto porque estas estresada?
Entonces agrega un paso extra de el tu teniendo
que controlarte.
Entonces you quiero esta comida porque yo no he comido el desayuno,
Yo tengo hambre, yo necesito combustible, necesito nutrientes.
Y luego yo tambien me pregunte que estoy anhelando,
entonces podra ser lo que sea que quiera.

Chinese:
再把餅乾放上去
我來跟你們說說如何用心吃
我鑽研十天
目前為止都很滿意
吃之前我問自己幾個問題
1. 此刻的心情如何, 我現在覺得很平靜

2. 你為何想進食
雖然這問題很怪
你得意識到你為甚麼要吃
是因為肚子餓嗎?
還是因為距離上一餐有段時間了呢?
還是你找個人來陪你抒發甜食欲

還是無事可做?
壓力大呢?
添加好幾個步驟來檢視你自己

我想吃因為我還沒吃早餐
我好餓喔! 吃點東西來補充能量
我問問自己我想要吃甚麼
想吃很多我想吃的東西

English:
And then we plop the little patty on,
so I'm gonna show you guys how
I have been mindful eating.
It's something that I've
been implementing for a week
and a half now and I've
been liking it so far.
Before I eat, I asked myself
a couple of questions.
So the


first question
is two to three emotions
that you're feeling, so
right now I feel calm,
I feel peaceful.
The second question is
why you want the food.
So this maybe like a weird question,
but it makes you aware of
like, why you're eating?
Are you eating this because you're hungry?
Has it been like a while
since your last mealtime?
Who are you eating this because
you're craving something sweet?
Are you eating this because you're bored?
Are you eating this
because you're stressed?
So it adds that extra
step of the you having
to check in with yourself.
So I want this food because
I have not eaten breakfast,
I am hungry, I need fuel,
I need some nutrients.
And then I also asked
myself what I'm craving,
so it could be anything
I eat whatever I want.

English:
As long as I explain why,
and like what I'm craving,
I want something that has hotRavella Venkatarama Rao

Ravella Venkatarama Rao (30 January 1927 – 10 December 2013) was a Telugu-language writer and poet. He wrote under pen-name, Jayasri.

Life
He was born in Gokinepally village, Mudigonda, Khammamet (now Khammamm) Hyderabad State.  He fought in the Telangana armed struggle against the Nizam and feudal lords. He was jailed for four years. He is survived by wife and four sons wife name is Ravella suguna and sons

 → Ravella Venkatarama Rao  →   Ravella Suguna
 → Ravella Madhava Rao      →   Ravella Aruna        →  Ravella Sri Navya, Ravella Sridivya,  Ravella Sri Kavya
 → Ravella Maheshwar Rao    →   Ravella Vimala


took off running.

Breton said Mulvihill, 45, was trying to reclaim his life after Lovejoy had claimed that he had molested their young son and sexually abused her. The couple had been separated since July 2014 and were in the final stages of completing their divorce.

Carlsbad police determined that the phone used to call Mulvihill was purchased by Lovejoy, and feces found in the bushes at the scene of the shooting were traced to McDavid, the prosecutor said.

Investigators found a multitude of guns and a silencer in McDavid’s garage, and a “blast bag” containing seven spent shell casings, Breton told the jury.

McDavid’s attorney, Ricky Crawford, said his client was a trained marksman and former Marine who fired his rifle only after he heard someone yell “I have a gun!”double eyelids. I saw a television program documenting the proliferation of foot ailments in women walking the streets in heels. The dress style seems to be the reverse of many Western trends; women never wear clothing that would expose their chests, but even fashionable adult women sport short shorts or skirts with tights. Men attend to their personal appearance, both complexion and attire, in a manner that is certainly attractive but likely creating pressure for their sex as well. Perfect has a number – somehow, the ideal height has been deemed 180 cm, precisely. And directly centered inside the school entrance is a full-length, elaborately carved mirror. What message does that send?

As an undergraduate student, I contributed to planning a special program called Carolina Beautiful Week. This program,


area in that ventral mesentery that connects to make sure you that ab will be best-known mainly because this ventral mesogastrium.12]

The minimal omentum is definitely produced, by just a loss for this mesoderm and ventral mesogastrium, of which hooks up your digestive system and duodenum in order to that anterior stomache walls. Simply by typically the subsequent increase with any lean meats this kind of leaf of mesoderm might be shared inside cost management sections – any less omentum between any belly and also failing liver, as well as a falciform plus coronary ligaments in between your ailing liver and additionally the particular tummy wall together with diaphragm.12]

Clinical significance

Clarifications about your mesenteric composition get caused the improved comprehending associated with diseases involving this mesentery, samples in which unfortunatelyplus wide-ranging mesenterectomy (high tie) that will significantly lower break in your mesentery along with free up lymph nodes give in.

Practical application for this T/CME cuts down on city five-year repeat rates throughout intestinal cancer malignancy coming from 6.5% to make sure you 3.6%, even though cancer-related five-year coping fees for folks resected pertaining to benefits because of by using the actual world-wide-web essay heightened through 82.1% to help 89.1%.18]

Radiology

Recent radiologic appraisals for your mesenteric body organ have long been engaged in during that wording regarding any modern realizing connected with mesenteric body anatomy.

When the body is usually divided inside non-flexural in addition to flexural countries, a lot of these can easily effortlessly always be differentiated inside everything that is without a doubt any mesentery men and women


ADDIS ABABA (Reuters) - The United Nations chief said on Monday he believed the need for U.N. sanctions against Eritrea will no longer exist following its peace deal with Ethiopia.

“The sanctions were motivated by a number of events that took place, (but) it is my belief that those events will no longer exist ... If the reasons that led to the sanctions will no longer exist ... they will naturally become obsolete,” Antonio Guterres told reporters in Addis Ababa.

Guterres was visiting the Ethiopian capital a day after Prime Minister Abiy Ahmed and Eritrea’s President Isaias Afkwerki declared that a “state of war” since a 1998-2000 conflict had given way peace and vowed to restore diplomatic and trade links.described previously \[[@CR17]\]. Briefly, PCR for seven housekeeping genes (*rpo*B, *gap*A, *mdh*, *pgi*, *pho*E, *inf*B and *ton*B) was conducted and the products were directly sequenced and analyzed for single loci variants.

*Location of the* CTX-M-15 *gene* {#Sec5}
---------------------------------

Conjugation was performed using plate mating experiments as described \[[@CR18]\] . Plasmid analysis was done using the method described \[[@CR19]\]. The DNA was transferred on a polyvinyl-based membrane using overnight capillary transfer (CUMC Protocol for Southern Blot, New York) and hybridization done using a CTX-M-15 DIG labelled probe (DIG High Prime DNA Labelling and Detection Starter Kit II, Roche, Germany) following manufacturer's instructions.

The whole genome sequence of chromosomal DNA of isolate number 39 was determined. Chromosomal DNA was isolated from an overnight culture with the Purelink DNeasy kit according to the manufacturer's instructions


woman washes her husband's face and feet and pours him a cup and prepares his bed and stands and serves her husband. And any woman who refrains from doing any of these tasks that she is obligated to perform - is forced to do them." Some recommend forcing her with a whip or by starvation "until she gives in."

And needless to say, she is at her husband's disposal whenever he is overcome by a desire "to satisfy his urges with her." And if she continues to rebel, he always has the right "to divorce her without her consent."

And there are many similar halakhot, only a few of which we have collected here. Nor have we cited everything in the name of the ones who said them, for lackof space. The readers are invited to find the references on Shabbat - and to browse around - on their own; this is a good opportunity for study. We will direct your attention to Tractate Shabbat, which does a good job of summing up halakha's attitude toward women: "a sack full of excrement" with a bleeding hole.

Some people will seek to console themselves: It's true that this is the halakha both m'doraita (from the Torah ) and m'drabanan (from the rabbis ), but that is not what is taught nowadays. But it suffices to listen to the sermon the sage Rabbi Ohvadia Yosef delivered five years ago, based on the well-known halakhic work "Kitzur Shulchan Aruch": "A man must take care not to walk between two women or


in obtaining a password to access classified material.

Baher Azmy, an attorney for Assange with the Center for Constitutional Rights, said the government’s evidence is not verifiable. “We have no access to and cannot review or see the government’s evidence,” he told the Washington Post. “We do not know if it is reliable.”

The prosecution also played a video purportedly showing a member of Al Qaeda urging militants to study WikiLeaks material. “The solution for Jihadis is to head to the free Internet,” the narrator declares.

“Manning was a trained analyst,” Fein said. “He knew Al Qaeda was an enemy of the United States. He knew they collected information from the Internet. He knowingly gave information through WikiLeaks to them.”

“Manning gave the enemy of the United States unfettered access to classifiedas Dreamhost, HostGator, and Hover.com, and Name.com have offered similar SOPA-related promotions.

"GoDaddy appears to be returning incomplete Whois information to Namecheap, delaying the transfer process" in violation of rules established by ICANN, the Internet Corporation for Assigned Names and Numbers, NameCheap wrote in a blog post today. By this afternoon, the company said that GoDaddy had "finally unblocked our queries" and that transfers should now "go smoothly."

For its part, GoDaddy, which has reportedly called customers to ask them to return, denies any wrongdoing. In a statement sent to CNET this afternoon, the company said:

Namecheap refused to say who at GoDaddy was contacted or when. Instead, chief executive Richard Kirkendall sent CNET a prepared statement saying "all we know on our side is that GoDaddy was preventing us from


urged Internet users and companies to "boycott GoDaddy until they send a letter to Congress taking back any and all support of the House and Senate versions of the Internet censorship bill, both SOPA and PIPA." The Protect IP Act, or PIPA, is the Senate version of SOPA.

On December 23, GoDaddy partially caved, announcing that it was no longer backing SOPA, but stopping short of saying it will oppose the legislation. And not until today did it post a clarification saying GoDaddy "does not support" Protect IP, either. (The GoDaddyBoycott.org site, which hasn't been updated, continues to say that GoDaddy endorses the Senate bill.)

A GoDaddy representative did not respond to a question from CNET this afternoon asking whether the company now opposes SOPA and Protect IP.

SOPA, of course,that ensued when the record magnitude-9 earthquake knocked out power and buckled roads before the tsunami flooded backup generators. Radiation fallout from the reactors forced the evacuation of about 160,000 people. The government has yet to say how many can return and when.

Jun Oshima, a spokesman for Tepco, declined to comment on the report as the utility is checking the contents, he said.

Hotlines between the central control room and the reactor buildings worked following the quake, while workers outside the buildings could use a total of nine transceivers, spokesman Masato Yamaguchi said yesterday. The company added 29 transceivers on March 13 and 80 more on March 15, Yamaguchi said.

A Chinese court has handed down a 10-year jail sentence to Chen Xi, the second dissident in four days to


be punished by death by stoning and crimes such as murder, rape, armed robbery, apostasy and drug trafficking are all punishable by hanging.

The European Union called Ashtiani's stoning sentence "barbaric." The Vatican pleaded for clemency and Brazil offered her asylum. The case further strained Tehran's relations with the West, already at odds over Iran's disputed nuclear program.

Two reporters for German newspaper Bild am Sonntag were detained in Iran in October last year when they were interviewing Ashtiani's son without official permission, highlighting the sensitivity of the case. The two were released in February.

Iranian authorities dismiss allegations of rights abuses, saying they are following Islamic law.

Mohammad Javad Larijani, secretary-general of the Iranian High Council for Human Rights, argued in December that stoning should not be classified as a methodof execution but rather a method of punishment which is actually more "lenient" because half of the people survive, the U.N. quoted him as saying. Reuters

As Amnesty International calls for an end to child executions in Iran I wonder to what avail their pleas.Little I shouldn't wonder, the regime in Iran is addicted, it feeds it's addiction with the life's blood of it's victims, no horror is too great to feed it's insatiable appetite for cruelty, pain and misery.It is a beast that has no place on this earth, but it is a special beast, it is a thinking beast, it thinks enough that it will not stop it's evil but try to hide it from the world by carrying out it's unspeakable barbarity behind the walls of


the Pope, speaking from the Basilica in Saint Peters, had this to say about Christmas.

"Let us ask the Lord to help us see through the superficial glitter of this season, and to discover behind it the child in the stable in Bethlehem, so as to find true joy and true light."

Friday, December 23, 2011

The Christmas War on Atheism: What's the Religious Right Whining About When It's Really Non-Believers Who Are Under Attack?

Right-wing Christians are waging a war on non-believers' right not to have religion shoved down their throats.By Alex DiBrancoDecember 22, 2011

It’s that time of year when, if you tune into Fox News or right-wing talk radio, you’re sure to hear the phrase “war on Christmas” repeated ad infinitum. Perhaps you’ve also seen Rick Perry’s new war onthemselves miserable in the process." Go to page two

No Justice for 62-Year-Old Man, Pepper Sprayed to Death by Florida Police

Nick Christie, 62, was arrested near Tampa for public intoxication about two and a half years ago. Concerned about Christie's recent erratic behavior, his wife had asked police to take her drunk husband to the hospital -- where he could take his medication -- rather than haul him off to jail. But Lee County Sheriff's Office police had another idea: They took Christie down to the station where they stripped him naked, covered his face with a spit mask, and paper sprayed him, until he died.

The District 21 Medical Examiner ruled his death was a homicide because he had been restrained and sprayed with pepper sprayed by law enforcement


of course I am, I don't deny the man in the slightest, his right to live in any way he chooses. It's just not a lifestyle that I could honestly recommend.

I do need to post an update from Fukushima, there is important news coming out of there. I shall look to it presently.

But in the meantime, a light hearted approach to a far from light hearted situation. We may as well laugh about it as not, whatever our approach, the situation on the ground will remain the same.

MIHARU VILLAGE, Fukushima Prefecture — As bitter winds blow around cesium and other radioactive particles spewed from the nearby Fukushima No. 1 nuclear power plant's reactors, Naoto Matsumura lights a cigarette, which he considers relatively good for his health.

In the zone:to die. I'm not going to leave here."

Indeed, this silver-haired, soft-spoken man of the land who has enjoyed playing golf in Saipan and the Philippines, says he now views himself as a lone maverick in a toxic desert — one hunted by an invisible enemy called "radioactivity" eating away at living things now and into the future. As the other animals perish around him, he wonders when it will be his turn.

All Matsumura's friends have left, and they no longer ask him to bring their stuff to them in the temporary shelters they must now inhabit. The automatic vending machines, which used to light up the country roads, no longer work.

After sunset, he is surrounded by miles of total darkness devoid of human movement. He has no television


the mud for political reasons," Bush said in Crawford, Texas.

''The effects of radiation do not come to people that are happy and laughing. They come to people that are weak-spirited, that brood and fret.''

“I think the Iraqi people owe the American people a huge debt of gratitude, and I believe most Iraqis express that. I mean, the people understand that we've endured great sacrifice to help them. That's the problem here in America. They wonder whether or not there is a gratitude level that's significant enough in Iraq.”

Bush interview 60 Minutes 1/14/07

“All hurricanes are acts of God because God controls the heavens. I believe that New Orleans had a level of sin that was offensive to God and they were recipients of the judgment of God for that.”connecting to Google when using HTTP instead of HTTPS. This is especially noticeable on the first connection attempt and less so afterwards as information get cached to speed up the connection.

Another reason are environments where all network traffic flows through a filter first that may block traffic that cannot be identified because of the use of HTTPS and the domain not being on a whitelist of sorts.

It may also be useful in edge-cases where HTTPS connections to Google are very slow or unstable while HTTP connections work fine.

In most cases, it makes little sense to use HTTP instead of HTTPS, but if you encounter a situation where HTTPS is not working or barely working, then you may find this option useful as it may provide you with access


in:1hr35min

Sift the flour into a mixing bowl. Add the lard and rub it in with your fingertips until thoroughly incorporated. Stir in the salt, then add the water and stir well to form a smooth dough.

Turn out onto a lightly floured work surface and knead for 2–3 minutes. Wrap the dough in cling film and chill for about 1 hour.

Divide the dough into 10 pieces and shape each one into a ball. Roll out each ball on the lightly floured surface, keeping an even round shape, to make a paper-thin disc about 15 cm (6 in) in diameter. As they are rolled, stack them up, interleaved with sheets of greaseproof paper.

Heat a griddle or cast-iron frying pan over a moderate heat until very hot. Cook the tortillas, oneParents admit to distracted driving with kids present

Monday, May 13, 2013

According to survey results presented by the Pediatric Academic Societies at its recent annual meeting, many parents on the road are putting their children at risk. The survey of more than 600 parents was conducted by the University of Michigan and investigated driving behavior when it comes to distractions, child restraints and previous driving history.

Michelle Macy, clinical lecturer in the Departments of Emergency Medicine and Pediatrics at University of Michigan and C.S. Mott Children's Hospital, said that while there is a focus on curbing distracted driving in teens, the survey showed that parents are more frequently distracted while driving their children aged 1 to 12 years old and that these drivers were more likely to have been in


dependent on bLPO concentration. The enhancement of enzymatic activity of HEWL by bLPO was affected by pH and ionic strength. The addition of potassium thiocyanate and hydrogen peroxide did not lead to an additional enhancement of the enzymatic activity of HEWL, as compared with bLPO alone. HEWL displayed candidacidal activity in all 3 strains of C. ablicans. The addition of bLPO alone did not affect the candidacidal activity of HEWL, but the bLPO system enhanced candidacidal activity of HEWL in all 3 strains of C. ablicans. bLPO enhanced the enzymatic activity of HEWL, but the bLPO system did not show additional enhancement of the enzymatic activity of HEWL. The addition of bLPO did not affect the candidacidal activity of HEWL, but the bLPO system did enhance the candidacidalSilver African Coins

Silver African Coins - This new partnership between the Scottsdale Mint and the Republic of the Congo began in 2015 to release a unique Silverback Gorilla coin that carries a face value of 5000 francs. APMEX is proud to offer a variety of products from the Scottsdale Mint.

Scottsdale Mint is known for their exquisite, unique Silver products and this new partnership is no different. This annual program is sure to generate plenty of interest from current Scottsdale Mint purchasers and brand new collectors.

To view available Silver African Coins or to get Silver prices, click on the image below representing the Silver Congo Coin of your interest:

This is the fourth release for the series of Republic of Congo Silverback Gorilla Silver coins minted by Scottsdale Mint. These


the Republic of Congo Silverback Gorilla Silver coins. These coins have a limited mintage, which further adds to their collectibility and strong numismatic....
Read More

This is the very first coin of a series released by the Scottsdale Mint for the Republic of Congo. These coins have a limited mintage, which further adds to their collectibility and strong bullion appeal.
Read More

Sold out from the mint! From Scottsdale Mint comes a new limited mintage Silver bullion coin from the Republic of Ghana, a nation on West Africa's Gulf of Guinea, known for diverse wildlife and secluded beaches.
Read Morethe skies to hint

The promise of a clerkship in the Mint.

_Fogarty Weffing_.

Hornet, _n_. A red-hot meteor of many tons weight, which sometimes hits a fellow unexpectedly between the eyes and knocks him silly. It is represented symbolically, as an insect with a bald head and an influential tail, but the man who has incurred a hornet shot out of a clear sky is not satisfied with that kind of representation, and avers with feeling that an instantaneous photograph of a hornet in flight would tell a different story.

Horrid, _adj_. In English, hideous, frightful, appalling. In Youngwomanese, mildly objectionable.

There was a pretty girl.

In the terror and the whirl

Of the tempest of her passion she was torrid!

But when moderately moved

By what she disapproved

She said, with gentle censure, it was horrid.

Horse,


is held with greatest tenacity by those most accustomed to the mischance of falling into adversity, and is most acceptably expounded with the grin that apes a smile. Being a blind faith, it is inaccessible to the light of disproof—an intellectual disorder, yielding to no treatment but death. It is hereditary, but fortunately not contagious.

Optimist, _n_. A proponent of the doctrine that black is white.

A pessimist applied to God for relief.

"Ah, you wish me to restore your hope and cheerfulness," said God.

"No," replied the petitioner, "I wish you to create something that would justify them."

"The world is all created," said God, "but you have overlooked something—the mortality of the optimist."

Oratory, _n_. A conspiracy between speech and action to cheat the understanding. A tyranny tempered by stenography.

Ordinary, _adj_. Common;ear,

And always I'd find a smile in the jeer;

In the frown a leer,

A grin in the tear

From the eyes of Sense and Truth.

From trammels of taste that hamper the sane

From my curst identity's chafing chain,

Free me, O Fate, at a stroke.

Make me a fool with a fool's grimace,

Narrow my forehead and widen my face,

The Joker has place

In the ranks of Grace.

But it's O, to be a Joke.

A34.

WHEN BOOKER WASHINGTON WAS LYNCHED IN BOSTON.

The lynching of Mr. Booker Washington, in Boston, was attended with few of the disagreeable incidents which have so frequently marred similar spectacles in the South. Regrettable as was the act itself, and inconvenient as it must have been to Mr. Washington, he who is most jealously devoted to the honor and good name of the


who would be benefitted by resumption. DEBTOR, n—a worthy person, in whose interest the national finances should be so managed as to depreciate the national currency."

B14.

Following are sample definitions from an unpublished dictionary for which (in behalf of the author) I am ready to receive subscriptions: _"Love_ , the folly of thinking much of another before one knows anything of oneself." _"Courtship_ , the timid sipping of two thirsty souls from a goblet which both can easily drain but neither replenish." _"Marriage_ , a feminine device for imposing silence, whereby one woman is made to guard the good name of a dozen others." _"Divorce_ , a resumption of diplomatic relations and rectification of boundaries."

B15.

Most of these dismally indecent pictures, it is almost needless to say, are photographs ofstudied "in-silico" by analyzing RNA binding motifs of RBPs, as reviewed in \[[@CR8]\]. RBPs' binding motifs are usually represented by position weighted matrices (PWMs) that provide the probability of having a specific nucleotide in each motif's position. PWMs are gathered from different databases \[[@CR9]--[@CR13]\] and scanned into the genome to find putative binding sites. The weakest step of this pipeline is the identification of the specific binding sites for the RBPs. PWMs are usually short (\>40% PWMs are shorter than 7nt) and provide low specificity \[[@CR8]\]. This precludes hits that are statistically significant. Implying that binding rules are diffuse (at least, on the PWM level), binding is probably co-defined by contextual information.

RBP--RNA interactions can be also experimentally identified \[[@CR14]\] by employing cross-linking and immunoprecipitation (CLIP) coupled with high-throughput


available, as opposed to 123 RBPs that have PWMs with significant hits in the transcriptome.

The results were encouraging: although the cell lines in the CLIP database did not match the cell lines of the experiments, it was possible to recover the depleted splicing factors. Previously, we found that this improvement also occurs combining CLIP experiments from different species \[[@CR8]\].

With CRISPR-Cas9 being more accessible, this pipeline could also be validated knocking out a splicing factor using this technique. In this case, the statistical approach should be changed accordingly. Using CRISPR, the knocked out gene -in this case a splicing factor- does not necessarily change its expression. Therefore, the statistical part related with changes in the expression should not be included and only the statistical part related with CLIP experimentsdefined as the relative expression of one path of the event against the expression of the reference, as follows:$$\documentclass[12pt]{minimal}
                \usepackage{amsmath}
                \usepackage{wasysym}
                \usepackage{amsfonts}
                \usepackage{amssymb}
                \usepackage{amsbsy}
                \usepackage{mathrsfs}


(*n* = 937). CLIP experiments were downloaded from three CLIP databases: POSTAR2 \[P\], CLIPdb \[C\], DoRiNA \[D\] and StarBase \[S\]. For each RBP, the table includes: number of experiments (\#Ex), database(s) that include the RBP (DDBB) and number of splicing events where the RBP binds after integrating all CLIP experiments. (XLSX 9261 kb) Additional file 2:**Figure S3. (Right-hand side)** Pearson correlation coefficient heatmap representing the similarity of RNA-binding protein binding sites in splicing events. Red and blue indicate higher and lower correlation, respectively. **(Left-hand side)** Information of the four experiments analyzed in this manuscript (Table [1](#Tab1){ref-type="table"}). KD-SRSF1 (green), KD-TARDBP (blue), KD-TAF15 (yellow), and KD-FUS (red). Each experiment shows two color lines: Expression *p*-value \<1e-3 (dark color) and CLIP *p*-value \<1e4 (light color). The CLIP *p*-value of KD-TAF15 (lightAR. Analysis and interpretation of data (e.g., statistical analysis, biostatistics, computational analysis): FC, MG, JF and AR. Writing, review, and/or revision of the manuscript: FC, MG and AR. Study supervision: AR. All authors read and approved the final manuscript.

This work was supported by the Provincial Council of Gipuzkoa through the MINEDRUG project and the Basque Government with the grant promoting doctoral theses for young pre-doctoral researchers \[grant numbers: PRE_2017_2\_0033 to FC\]. The funding source had no involvement in the design of the study, collection, analysis and interpretation of data or in the writing of the manuscript.

The source code, databases and a tutorial are available in the GitLab repository, <https://gitlab.com/fcarazo.m/sfprediction>.

Not applicable.

Not applicable.

The authors declare that they have no competing interests.


this year, where there was a well-coordinated opposition to affording several endangered species protection under CITES. In particular, there was a high resistance to listing commercially-exploited marine species under CITES. Come along and find out the reasons given as to why unsustainable exploitation levels should not be reigned in, and hear some insights into how politics play out in international forums such as these!

· Radio Access:

Humanist Outlook, Future broadcasts at 10.30am, 783 kHz Wellington, on Saturday, 26 June, 24 July and 21 August, 18 September, & 16 October.
If you are outside the Wellington radiobroadcast area, go to www.accessradio.org.nz to listen or to download a pod cast after the event.
Please note: the time slot was changed at Radio Access’s suggestion. Humanist Outlook is now broadcast 10:30 am on Saturdaycontrol, it should always remain so. This is a classic case of different religions putting forward incompatible claims, and it illustrates the need for completely secular law.

Interestingly, ultra-orthodox Israeli Jews claim and receive exemption from military service, even though much of the effort of Israel’s armed forces goes into supporting their territorial ambitions or dealing with their consequences.

And what of Christianity?

Historically, Christianity dominated much of Europe and western Asia from the time in the fourth century CE when it was adopted as the official religion of the Roman Empire. Before then, Christians were sometimes persecuted as atheists, because they did not accept the prevailing polytheism and refused to worship Emperors. However, once they were in a position of power, they set about persecuting others: polytheists, atheists, and apostates.


field of human rights. They have worked energetically to make people view it as equivalent to racism.

The Vatican thought that this was a good idea and came up with the idea of “Christianophobia” to describe the attacks of secularists on the Christian religion and its entrenched power. Of course, genuine persecution of Christians does take place in a number of countries, including Pakistan, Egypt and Nigeria. IHEU has joined with other NGOs to support their cause. But attempts to show that Christians are persecuted in the west have descended to the level of farce.

The BBC recently broadcast a very biased programme called “Are Christians being persecuted?” discussing the views of some UK Christians who claim persecution by the law. After trotting out a few feeble examples, the programmeare the most promising for true-to-life high fidelity, are destined
  for headphone reproduction. Even in their heyday they found no place in routine
  recording and broadcasting. At that time the causes were unreliable frontal localization,
  incompatibility with loudspeaker reproduction, as well as their tendency to
  be unaesthetic. Since digital signal processing (DSP) can filter routinely using binaural
  head-related transfer functions, HRTF, dummy heads are no longer needed.
Still the most common application of headphones is to feed them with stereo signals
  originally intended for loudspeakers. This raises the question of the ideal frequency
  response. For other devices in the transmission chain (Fig. 14.1), such as microphones,
  amplifiers and loudspeakers, a flat response is usually the design objective, with easily
  definable


file editor like Audacity or snd, and delete one single 44100 Hz sample from just one channel, and realign the other channel so that the first sample now happens with the second one of the edited channel, and play it back.
You will hear a very noticeable difference, even though the difference is a time delay of only 1/44100th of a second.
Consider this: sound goes about 340 mm / ms, so at 20 kHz this is a time error of plus minus one sample delay, or 50 microseconds. That's 17 mm of sound travel, yet you can hear the difference with that missing 22.67 microseconds, which is only 7.7 mm of sound travel.
The absolute cut-off of human hearing is generally regarded to be around 20 kHz, soQ:

Particular producer-consumer scenario

I need to implement a particular producer-consumer scenario in which the class Consumer creates two thread handling two ports, each one store a value into the corresponding port and, if a value is available on the other port, process is called to consume the values on both ports. This is my attempt:
#include <thread>
#include <atomic>
#include <condition_variable>
#include <vector>
#include <iostream>
#include <mutex>

struct Port {
    int value;
    std::atomic<bool> free;
};

class Consumer {
private:
    Port port1;
    Port port2;
    std::mutex mutex;
    std::condition_variable port1_ready;
    std::condition_variable port2_ready;

    std::vector<std::thread> workers;
    std::atomic<bool> done;
    int count;

public:
    Consumer() : done(false), count(0) {


Ewing's sarcoma of the mandible.
Cure rates of Ewing's sarcoma have improved from 10% to 75% with the use of a combination of local therapy and aggressive multi-drug chemotherapy, to decrease both the incidence of local disease recurrence and the development of pulmonary and skeletal metastases. In Ewing's sarcoma of the mandible, complete surgical resection is preferred to irradiation for local control in those instances when surgery can be performed with minimal loss of function and disfigurement.From his Columbia University web site comes the latest manifesto from ex NASA scientist Dr. James Hansen, who calls for a “centrist” third political party. I’ve republished the manifesto below. h/t to Charles the moderator.

The American Party

James Hansen 29 May 2013

My remarks when receiving the Ridenhour Courage Award were written in Union Station on my way to the event. But my concluding comment — that we are near a point when the American people should contemplate a centrist third party — was not an idle spur-of-the-moment reflection.

I was in government 40 years, long enough to understand how aging organizations can evolve into self-licking ice cream cones, organizations whose main purpose becomes self-perpetuation rather than accomplishment of their supposed objectives. The public can see this tendency in our politicians,


spreads a pilot signal with a unique code. A signal combiner 503 multiplexes the spread user signals supplied from the user signal spreading circuits 501-1 to 501-K and the pilot signal from the pilot signal spreading circuit 502. A transmission antenna 504 transmits an output from the combiner 503.
Referring to FIG. 11B, in the receiver, a pilot correlator 601 detects the pilot signal from the received signal transmitted from the transmission antenna 504 and received by a reception antenna (not shown), and extracts the reception timings of the user signals and transmission channel parameters. Similarly, a user signal correlator 602 detects a desired user signal from the received signal. A coherent detector 603 demodulates a user signal by using the transmission channel parameters extracted from the pilot signal.with *L. reuteri* I5007 culture supernatant in the absence of LPS caused no significant change in TNF-α and IL-6 mRNA expression. However, the culture supernatant of *L. reuteri* I5007 did reduce LPS-induced inflammatory cytokine expression during 8--24 h (Figure [3](#Fig3){ref-type="fig"}). In general, both mRNA expression and protein levels of claudin-1, occludin and ZO-1 in cells pre-treated with the culture supernatant *of L. reuteri* I5007 were higher than those in cells induced by LPS after 4 or 8 h (Figure [4](#Fig4){ref-type="fig"} and Figure [5](#Fig5){ref-type="fig"}).

Discussion {#Sec8}
==========

The defense response of the intestinal epithelium against pathogenic bacteria has been extensively explored, and evidence suggests that interactions between commensal bacteria and the host are involved in this process \[[@CR33]\]. Thus, much attention has been paid to the effects of probiotics on gut barrier function. Some probiotics might improve


PCR System (Applied Biosystems, Singapore) with SYBR Green PCR Master Mix (Takala, Ostu, Japan), containing MgCl~2~, dNTP and Hotstar Taq polymerase. Briefly, copy numbers were calculated relative to a dilution series (1:10^7^ to 1:1) of the respective reference plasmids which contained the cloned RT-PCR products obtained with these primers. The PCR system consisted of 5.0 μL of YBR Green qPCR Mix, 1.0 μL of cDNA, 0.25 μmol of each primer, and 3.6 μL of double distilled water in a final volume of 20 μL. Each sample was determined in triplicate and the housekeeping gene, β-actin, was used as the internal standard for the PCR reaction.Table 1**Primers used for real-time PCRGenesPrimersSequences (5\'-3\')Size (bp)Tm (°C)**TNF-αForwardATTCAGGGATGTGTGGCCTG12062ReverseCCAGATGTCCCAGGTTGCATIL-6ForwardTGGATAAGCTGCAGTCACAG10954ReverseATTATCCGAATGGCCCTCAGClaudin-1ForwardGCAGCAGCTTCTTGCTTCTC66458ReverseCTGGCATTGACTGGGGTCATOccludinForwardATCAACAAAGGCAACTCT15750ReverseGCAGCAGCCATGTACTCTZO-1ForwardGAGTTTGATAGTGGCGTT29850ReverseGTGGGAGGATGCTGTTGTβ-actinForwardTGCGGGACATCAAGGAGAAG21660ReverseAGTTGAAGGTGGTCTCGTGG

Extraction of protein and immunoblotting {#Sec16}
----------------------------------------

Total protein was extracted from the intestinal tissues or IPEC-J2 cells using lysis buffer (150 mM NaCl,Pseudocolumns

PL/SQL recognizes the SQL pseudocolumns: CURRVAL, LEVEL, NEXTVAL, ROWID, and ROWNUM. In PL/SQL, pseudocolumns are only allowed in SQL queries, not in INSERT / UPDATE / DELETE statements, or in other PL/SQL statements such as assignments or conditional tests.

CURRVAL and NEXTVAL

A sequence is a schema object that generates sequential numbers. When you create a sequence, you can specify its initial value and an increment. CURRVAL returns the current value in a specified sequence.

Before you can reference CURRVAL in a session, you must use NEXTVAL to generate a number. A reference to NEXTVAL stores the current sequence number in CURRVAL. NEXTVAL increments the sequence and returns the next value. To get the current or next value in a sequence, use dot notation:

sequence_name.CURRVAL
sequence_name.NEXTVAL

After creating a sequence, you can use it


was selected from a table. The first row selected has a ROWNUM of 1, the second row has a ROWNUM of 2, and so on. If a SELECT statement includes an ORDERBY clause, ROWNUMs are assigned to the retrieved rows before the sort is done; use a subselect (shown in the following example) to get the first n sorted rows.

You can use ROWNUM in an UPDATE statement to assign unique values to each row in a table, or in the WHERE clause of a SELECT statement to limit the number of rows retrieved:

The value of ROWNUM increases only when a row is retrieved, so the only meaningful uses of ROWNUM in a WHERE clause are

... WHERE ROWNUM < constant;
... WHERE ROWNUM <= constant;

SQL Operators

PL/SQL lets you use allthat has the appropriate fields (usually declared using %ROWTYPE):

-- This cursor queries 3 columns.
-- Each column is fetched into a separate variable.
FETCH c1 INTO my_empno, my_ename, my_deptno;
-- This cursor was declared as SELECT * FROM employees.
-- An entire row is fetched into the my_employees record, which
-- is declared with the type employees%ROWTYPE.
FETCH c2 INTO my_employees;

For each column value returned by the query associated with the cursor, there must be a corresponding, type-compatible variable in the INTO list. Typically, you use the FETCH statement in the following way:

The query can reference PL/SQL variables within its scope. Any variables in the query are evaluated only when the cursor is opened. In the following example, each retrieved salary is multiplied by 2, even though factor is incremented after every fetch:

If you


BY in the outer query.

You can use a subquery in place of a table name, in the FROM clause of a query. This technique lets you join a table with a small set of rows from another table, instead of joining the entire tables.

You can create a table or insert into a table, using a set of rows defined by a subquery.

DECLARE
CURSOR c1 IS
-- The main query returns only rows where the salary is greater than the average salary.
SELECT employee_id, last_name FROM employees WHERE salary > (SELECT AVG(salary) FROM employees);
CURSOR c2 IS
-- The subquery returns all the rows in descending order of salary.
-- The main query returns just the top 10 highest-paid employees.
SELECT * FROM
(SELECT last_name, salary FROM employees ORDER BY salary DESC, last_name)
WHERE ROWNUM < 11;
BEGIN
FOR personCursor Expressions

You cannot use a cursor expression with an implicit cursor.

Cursor expressions can appear only:

In a SELECT statement that is not nested in any other query expression, except when it is a subquery of the cursor expression itself.

As arguments to table functions, in the FROM clause of a SELECT statement.

Cursor expressions can appear only in the outermost SELECT list of the query specification.

Cursor expressions cannot appear in view declarations.

You cannot perform BIND and EXECUTE operations on cursor expressions.

Example of Cursor Expressions

In this example, we find a specified location ID, and a cursor from which we can fetch all the departments in that location. As we fetch each department's name, we also get another cursor that lets us fetch their associated employee details from another table.

DECLARE
TYPE emp_cur_typ IS REF


the SELECTFORUPDATE statement, you can explicitly lock specific rows of a table to make sure they do not change after you have read them. That way, you can check which or how many rows will be affected by an UPDATE or DELETE statement before issuing the statement, and no other application can change the rows in the meantime.

Using FOR UPDATE

When you declare a cursor that will be referenced in the CURRENTOF clause of an UPDATE or DELETE statement, you must use the FORUPDATE clause to acquire exclusive row locks. An example follows:

The SELECT ... FORUPDATE statement identifies the rows that will be updated or deleted, then locks each row in the result set. This is useful when you want to base an update on the existing values inlarge updates.

The next example shows that you can use the %ROWTYPE attribute with cursors that reference the ROWID pseudocolumn:

DECLARE
CURSOR c1 IS SELECT ename, sal, rowid FROM emp;
emp_rec c1%ROWTYPE;
BEGIN
OPEN c1;
LOOP
FETCH c1 INTO emp_rec;
EXIT WHEN c1%NOTFOUND;
IF ... THEN
DELETE FROM emp WHERE rowid = emp_rec.rowid;
END IF;
END LOOP;
CLOSE c1;
END;

Doing Independent Units of Work with Autonomous Transactions

An autonomous transaction is an independent transaction started by another transaction, the main transaction. Autonomous transactions do SQL operations and commit or roll back, without committing or rolling back the main transaction. For example, if you write auditing data to a log table, you want to commit the audit data even if the operation you are auditing later fails; if something goes wrong recording the audit data, you do not want the main operation to be rolled


transaction is rolled back.

Using Autonomous Triggers

Among other things, you can use database triggers to log events transparently. Suppose you want to track all inserts into a table, even those that roll back. In the example below, you use a trigger to insert duplicate rows into a shadow table. Because it is autonomous, the trigger can commit changes to the shadow table whether or not you commit changes to the main table.

-- create a main table and its shadow table
CREATE TABLE parts (pnum NUMBER(4), pname VARCHAR2(15));
CREATE TABLE parts_log (pnum NUMBER(4), pname VARCHAR2(15));
-- create an autonomous trigger that inserts into the
-- shadow table before each insert into the main table
CREATE TRIGGER parts_trig
BEFORE INSERT ON parts FOR EACH ROW
DECLARE
PRAGMA AUTONOMOUS_TRANSACTION;
BEGIN
INSERT INTO parts_log VALUES(:new.pnum, :new.pname);
COMMIT;
END;
-- insert a row into the main table,no database state."

-- create the debug table
CREATE TABLE debug_output (msg VARCHAR2(200));
-- create the package spec
CREATE PACKAGE debugging AS
FUNCTION log_msg (msg VARCHAR2) RETURN VARCHAR2;
PRAGMA RESTRICT_REFERENCES(log_msg, WNDS, RNDS);
END debugging;
-- create the package body
CREATE PACKAGE BODYq debugging AS
FUNCTION log_msg (msg VARCHAR2) RETURN VARCHAR2 IS
PRAGMA AUTONOMOUS_TRANSACTION;
BEGIN
-- the following insert does not violate the constraint
-- WNDS because this is an autonomous routine
INSERT INTO debug_output VALUES (msg);
COMMIT;
RETURN msg;
END;
END debugging;
-- call the packaged function from a query
DECLARE
my_empno NUMBER(4);
my_ename VARCHAR2(15);
BEGIN
...
SELECT debugging.log_msg(ename) INTO my_ename FROM emp
WHERE empno = my_empno;
-- even if you roll back in this scope, the insert
-- into 'debug_output' remains committed because
-- it is part of an autonomous transaction
IF ... THEN
ROLLBACK;
END IF;
END;


direct contribution to the load voltage from the Tx is much smaller than that of the repeater. This is because of two reasons: firstly, the mutual inductance $M_{\rm{tx\text{-}rx}}$ is much less than $M_{\rm{rp\text{-}rx}}$, secondly, the voltage contributions from the transmitter and the repeater are in 90 degrees out of phase.

Before moving to the multi-Tx WPT scheme, let us consider a numerical example of 3-coil WPT system with three identical coils (the coil parameters are defined in Table \[Table.coil\_parameters\]) in order to validate the above analytical results and approximations. The distances between the Tx to the repeater and the repeater to the Rx are $10$ mm and $50$ mm, respectively. In this example, $\Delta\approx 0.1$, which meets the requirement of (\[3coilcurrents\_smpl2\]). The normalized coil currents using the full analytical form in aredistribution of currents over the array resonators corresponds to a resonant mode at the operational frequency. This property ensures creation of independent, point-to-point energy channels across the “lens”. Note that, according to the above discussion, the current in a particular Tx decreases when the Rx is moved away from it.

![The coil arrangement of the multi-Tx WPT system.[]{data-label="multitx"}](graph/multiTX.pdf "fig:"){width="3.5in"}\

The geometrical configuration can be in any form, for example, 1D linear array, 2D coil pad, or even a 3D arrangement. The only requirement is to have negligible cross coupling between the channels. For example, a coil arrangement of a 1D linear array is shown in Fig. \[multitx\] and the Rx can be positioned (or moved) along the array. We design the Tx and repeater arrays with partially overlapping coils to ensure


zero mutual coupling between the adjacent coils. All the coils are chosen to be identical circular spirals with the design parameters given in Tables \[Table.coil\_parameters\] and \[Table.coil\_arragement\]. The working frequency of the WPT system is chosen to be at $1$ MHz and a series tuning capacitor is connected with each coil to tune the resonant frequencies to the working frequency. The transmitters connected in parallel and are excited by a voltage source.

\[Table.coil\_arragement\]

  Parameters                                                       to a point where the gene was originally thought to be essential \[[@pgen.1005577.ref004],[@pgen.1005577.ref006],[@pgen.1005577.ref011]\]. However, the pathogenesis of both *S*. *pyogenes* and *S*. *aureus* is severely affected by the deletion of RNase Y, in both silkworm and mouse models \[[@pgen.1005577.ref007],[@pgen.1005577.ref008],[@pgen.1005577.ref014]\], cementing the importance of RNase Y across a wide range of Firmicutes.

Several functions, that are not mutually exclusive, have been described for the *S*. *aureus* RNase Y: A 3\' phosphodiesterase activity, demonstrated *in vitro* \[[@pgen.1005577.ref015],[@pgen.1005577.ref016]\], utilises the same active site in the HD domain as the endoribonucleolytic activity which has been demonstrated *in vivo* by the identification of a single RNase Y cleavage site in the *sae* virulence regulator operon \[[@pgen.1005577.ref008]\]. *S*. *aureus* RNase Y has furthermore been shown by bacterial-two-hybrid assay to interact with enolase, a key metabolic


considered significant for this study.

^b^) Only RNAs that passed the quality criteria are included.

Non-coding RNAs are important components of bacterial gene regulation. The list of non-coding (ncRNAs), small RNAs and untranslated regions (UTRs) compiled by Sassi and coworkers \[[@pgen.1005577.ref027]\] (collectively referred to as ncRNAs throughout this manuscript, for simplicity) were therefore added to the analysis of open reading frames described above ([S1 Fig](#pgen.1005577.s002){ref-type="supplementary-material"}, [S1 Table](#pgen.1005577.s011){ref-type="supplementary-material"}). Only 67 ncRNAs passed the quality control criteria for confidently estimating their half-lives, mainly due to the silica-column based method of RNA isolation used for the RNA-seq, which depletes RNA molecules shorter than 200 nucleotides (nt). Among them, six ncRNAs (srn_1510_rsaA, srn_1520_Sau76, srn_2620_Teg61, srn_2830_SbrB, srn_3820_SprX and srn_3160_RsaOD/TB-glyS) were stabilised and accumulated above the 2-fold cut-off ([S1 Table](#pgen.1005577.s011){ref-type="supplementary-material"}). As expected, abundance of the 514membrane association is generally advantageous for bacterial RNA degradation machinery. While both ΔY and Y^367AA^ mutants exhibit growth-curves that are very similar to the WT strain, at 37°C in Mueller-Hinton broth, the anchorless Y^Δ2--24^ mutant grows significantly slower, and thereby indicates an important function of the membrane anchor that is dissimilar to the loss of enzymatic activity in the ΔY and Y^367AA^ mutants ([S3 Fig](#pgen.1005577.s004){ref-type="supplementary-material"}).

There are at least three potential functions of a membrane anchor, activity enhancement, activity limitation, and/or sequestering another protein.

1\) Is the membrane anchor needed for the endoribonucleolytic activity of RNase Y?

The Y^Δ2--24^ mutant was examined by EMOTE, and it became immediately apparent that the membrane anchor is not a requirement for RNase Y enzymatic activity. Every one of the 99 identified RNase Y cleavage


opposite to the stabilisation seen in the ΔY mutant ([Fig 1](#pgen.1005577.g001){ref-type="fig"}). However, the EMOTE data for strain Y^Δ2--24^ does not immediately reveal an increased activity, since such an effect can be accompanied by changes in expression levels or degradation by other RNases.

3\) Is the membrane anchor needed to anchor a protein partner of RNase Y to the membrane?

Both strain ΔY and Y^367AA^ have lost RNase Y activity, but whereas ΔY can neither bind other proteins nor anchor them to the membrane, the Y^367AA^ mutation presumably only affect the enzymatic activity of RNase Y itself, with no change in the intra-cellular localisation. The overall correlation between the EMOTE data of ΔY and Y^367AA^ is very strong (Pearson coefficient of 0.95; [S2 Table](#pgen.1005577.s012){ref-type="supplementary-material"}). Therefore, if RNase Y serves as anwas performed to demonstrate that the cleavage site and the ORF are on the same RNA molecule. Sometimes two ORFs could be connected (two ORF-names in second column), and the distance to each ORF is shown in the final column. (C) Agarose gels showing the RT-PCR product used to define (B), with each reaction performed either without or with the reverse transcriptase step. Primer sequences can be found in the oligonucleotide table.

(PDF)

######

Click here for additional data file.

###### RNA immediately adjacent to RNase Y cleavage sites is often, but not always, stabilised in the ΔY mutant.

300 nucleotides upstream and 300 nucleotides downstream of the 99 identified RNase Y cleavage sites were examined for stability in the RNAseq data, and plotted in a scatter-plot. The majority exhibits no significant


differentially, by allowing the decay of either the upstream or downstream genes, thus combining maturation and decay-initiation in a single cleavage event. Thus there are multiple outcomes of RNase Y endoribonucleolytic activity.

(PDF)

######

Click here for additional data file.

###### Predicted free energy of RNA folding near the RNase Y sites.

A\) The sub-sequences used for calculating predicted secondary structures near the 99 RNase Y cleavage sites. 50 nt upstream, 50 nt overlapping and 50 nt downstream of the sites. B) Box-plot showing the potential free energy of folding in the three sub-regions (dark grey). One sub-region of 50 nt upstream of the site (Upstream), one region of 50 nt straddling across the site (Overlapping), and one region of 50 nt downstream of the cleavage site (Downstream). As control, the sequencesthe RNA. N: Number of reads that correspond to that specific 5'-end. Q: Quantification value, i.e. number of unique Quantification Sequences, for that specific 5'-end (see also Redder, 2015 for a detailed explanation).

(ZIP)

######

Click here for additional data file.

###### Full EMOTE data for ambiguously mapping reads.

Seqnames: Accession number for the reference genome used. 5prime pos: Position on the genome where the first (5'-end) base of the RNA maps. Strand: Strand to which the RNA correspond. Nuc5p: The first (5'-end) base of the RNA. N: Number of reads that correspond to that specific 5'-end. Q: Quantification value, i.e. number of unique Quantification Sequences, for that specific 5'-end (see also Redder, 2015 for a detailed explanation).

(ZIP)

######

Click here for additional data file.

We would like to thank Stéphane Hausmann, Joshua Armitano


children with heart defects, which is why the health department has empanelled private hospitals.

There are now a total of 15 private hospitals that conduct such surgeries – eight in Jaipur, two each in Bharatpur and Udaipur, and one each in Jodhpur, Kota and Ajmer, reports TOI.

The health department conducts regular screening of children in government schools, anganwadis and madrasas, to see if they have any disorder. They identify those that might have a heart defect and conduct diagnostic tests. The department then helps those diagnosed with a disease in getting free treatment at the hospital.

Like this story? Or have something to share?

Write to us: contact@thebetterindia.com

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NEW: Click here to get positive news on WhatsApp!it and figure out what needs to be measured to further improve the business. Customer Development - Talking 1-to-1 with our customers and prospects, to get a better understanding of demands and needs in the market and how we can address them.

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tell by now, the work will be varied and it will also be very challenging.

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You will be expected to take initiative. Not everything you have to do will be clearly defined and prepared in bite-sized proportions. You'll simply start working as part of the marketing team and you'll be expected to contribute to the overall goal for Thrive Themes to create more awesome content and generate moreon enemies. During this process our Physics programmer was able to tweak and debug his hand crafted thruster analysis tool which greatly aids in placing and balancing thrusters for all future ships.

We’ve also been upgrading the Hornet to PBR so it has a lot more realistic look and feel to it. During this process Chris Smith took it upon himself to make some interior upgrades to the cockpit with higher resolution pass adding a lot more intricate details to the interior of the cockpit. Intricate mechanical workings were done as well to make the canopy operate on a believable track system and modifying the seat for a believable ejection. Lastly with PBR came the integration of the weathering system that allows him to apply some very believable wear


and tear looks to the models. He even took a pass of mocking up some different armor types for it. With all of these detailed changes plus the PBR conversion the Hornet is really looking quite stunning!

We’ve also grown our team here a bit over the last month with an additional three new employees bringing our total headcount in this studio to 27. Joining us this month were Kami Talebi (Production Coordinator), Joanna Whitmarsh (Marketing Associate), and James Pugh (Assistant Community Manger) who’ve all hit the ground running quite well! It has been amazing to watch the team here grow from three people almost a year ago to our current size today and we all look forward to continuing to grow the best possible team in the futureNEW DELHI: It took meetings with foreign minister S Jaishankar and senior MEA officials as well as persuasion by prominent Shia leaders to get Indians stranded in Iran, who are negative for Covid-19, to return home even as there was official confirmation that 255 Indian pilgrims have tested positive for the disease.A batch of 66 Indian pilgrims and some students who were in Iran arrived by a Mahan Air flight on Wednesday. Sources said a second flight with 270 Indians would arrive on Thursday. The pilgrims have been taken to a quarantine facility in Jaisalmer. Most pilgrims are from Ladakh which is now in a virtual lockdown, particularly after a soldier tested positive for the virus.Meanwhile, tweeting the story of a man thanking his government for ‘rescuing’ his


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of 58657099 and 0.4?
23462839.6
Work out -1797 * -34.99.
62877.03
0.3 * 1363.1746
408.95238
What is the product of -695 and -0.0559706?
38.899567
-63.2 * 8.8
-556.16
Multiply 1.525 and 0.2664.
0.40626
-72992984*-5
364964920
What is 0 times -729053?
0
Work out 7363059 * 0.2.
1472611.8
-3133.7 times -0.55381
1735.474397
What is the product of -1 and 11184125?
-11184125
Product of 411 and -10.49.
-4311.39
0.001677*-582
-0.976014
Multiply 44.9635 and 7.
314.7445
Work out 740685 * -0.18.
-133323.3
1.4927691*0.3
0.44783073
Calculate 5*-25636819.
-128184095
Work out 0.1 * -8588773.
-858877.3
1479*93125
137731875
259.319 times -12.1
-3137.7599
Calculate -5907689*2.
-11815378
Product of 10867766 and -5.
-54338830
What is the product of -6 and 24.32285?
-145.9371
What is the product of 0.4 and -0.089204?
-0.0356816
What is 13040.6 times 0.473?
6168.2038
Work out 0.7 * -46061.64.
-32243.148
Multiply -172.31 and 4.3.
-740.933
Calculate -3.5092*2363.
-8292.2396
3 times 7059.1024
21177.3072
Work out 0.2 * -35285396.
-7057079.2
Work out 0.23 * 13.97.
3.2131
Multiply 549161 and 0.01.
5491.61
What is the product of -53.5 and 131.142?
-7016.097
237 times -282327
-66911499
Product of 977511 and 2.4.
2346026.4
Multiply 69 and 111744.
7710336
Work out -5 * 1428570.
-7142850
What is the product of -0.3 and -228.810106?
68.6430318
Calculateis the product of -0.01 and 5223.98?
-52.2398
What is the product of 4710 and -8526?
-40157460
What is the product of 11590632 and 0.6?
6954379.2
65964 times -0.03
-1978.92
Work out -0.8 * -20407626.
16326100.8
Work out -1709908.07 * 0.4.
-683963.228
108.517401 * 0.2
21.7034802
What is 3 times 21077340?
63232020
Multiply -685706 and 1.4.
-959988.4
What is -2.7993 times -0.104?
0.2911272
-0.054 times -157.2
8.4888
Multiply 1.2 and 2559634.
3071560.8
What is -79.1 times 8.018?
-634.2238
-8 * 78104500
-624836000
Work out -245.53 * -0.05369.
13.1825057
12831199 times -0.2
-2566239.8
Multiply -4237 and -247784.
1049860808
What is the product of -14226644 and 0.5?
-7113322
Calculate -23804*2784.
-66270336
Work out 2884 * 56.
161504
What is -0.5 times -0.06398271?
0.031991355
0.084 times -1978.521
-166.195764
Product of -11 and -32053261.
352585871
Multiply 1018847 and