York.  Mack's
20-pounders commanded the bayou road, and Duryea went into battery
in advance of the centre, between Paine and Weitzel.

Weitzel's front line was composed of the 8th Vermont and 114th New
York, with the 12th Connecticut, 160th New York, and 75th New York
in the second line.  The guns of Bainbridge and Carruth went into
battery near the left flank, and working slowly kept down the fire
of the Confederate artillery in their front.  When the fire of
musketry became hot, Weitzel sent the 75th New York to try to gain
the canebrake on the left, in advance of the enemy's works, with
a view of turning that flank.  Of this movement Taylor says in his
report that it was twice repulsed by the 5th Texas and Waller's
battalion, under Green, and the 28ththe men might rest.  To relieve the
exhausted skirmish line, the 4th Massachusetts and the 162d New
York of Ingraham's brigade were sent forward from the reserve,
leaving him only the 110th New York.

By dawn the next morning, at all events, Banks calculated, the
turning column would be in place; accordingly during the night he
gave orders to assault along the whole front as soon as it should
be light enough to see.

However, shortly after midnight, sounds were heard on the picket
line, indicating some unusual movement behind the Confederate works.
When, at daybreak, the various skirmishers moved forward in eager
rivalry, they found the Confederates gone.  Captain Allaire, leading
his company of the 133d New York, was the first to enter the works;
the regiment itself and the 8th New Hampshire followed closely,
and the colors of


as the obstructions could be
removed, got under way and moved up the bayou abreast with the
advance of the army.

The losses of the Nineteenth Army Corps in this its first battle
were 3 officers and 37 men killed, 8 officers and 176 men wounded;
in all 224.  The 38th Massachusetts headed the list with 6 killed
and 29 wounded, and Gooding's brigade, to which this regiment
belonged, reported 87 casualties, or 38 per cent. of the whole.
In the six light batteries 15 horses were killed and 12 wounded,
and one caisson of the 1st Maine was upset and lost in crossing
the Teche to go into action.

The losses of the Confederates have never been reported and no
means are known to exist for estimating them.

The disparity of the forces engaged was more than enough toother hand, one turns to consider the
lost opportunity of Grover, two things stand out in strong relief:
the one, the positive disadvantage of employing forces, too large
for the affair in hand or for the scene of operations; the other,
that bold adventures must be carried boldly to the end.

Instead of making the campaign with four brigades and twenty-four
guns, as Weitzel's original plan had contemplated, Banks, for
greater security, set out with seven brigades and fifty-six guns.
So far as concerned the main body ascending the Teche, this excess
of strength could do no harm, but it was otherwise with the turning
column by the lake; for to the needless augmentation of the artillery
were directly due not only the day and night first lost, but also
the still more precious hours of daylight consumed in landing


his mind and to conclude that it would,
after all, be possible to march to Simmesport, and there, using
the light-draught boats of the Department of the Gulf, supplemented
by such steamers as Grant might be able to spare for this purpose,
to transfer the whole column to Grand Gulf and thence march to join
Grant in the rear of Vicksburg.  Accordingly, on the 13th of May,
Banks gave orders for the immediate movement of his whole force in
accordance with this plan, and set aside all the preparations that
had previously been made.

When the news reached Washington that Grant had gone to Jackson
and Banks to Alexandria, great was the dissatisfaction of the
Government and emphatic its expression.  On the 19th of May Halleck
wrote to Banks:

"These operations are too eccentric to be pursued.  Iafter the landing at Bayou Sara, a party of cavalry rode in,
bringing the news of Augur's battle of the 21st.  Hearing that
Augur was at that moment engaged with the enemy, Banks pressed
forward his troops.  In a violent storm of wind and rain Grover
pushed on until he met Augur's outlying detachments.  Then, finding
all quiet, he went into bivouac near Thompson's Creek, north-west
of Port Hudson.  Paine followed, and rested on the Perkins plantation,
a mile in the rear of Grover.  Banks made his headquarters with
Grover.  Augur covered the front of the position taken up by the
enemy after the battle of Plains Store.  On the same day, the 22d,
Sherman came up the river, landed at Springfield, and went into
position on the Bayou Sara road on


in the camp of the stormers.  Among
those that volunteered for the forlorn hope but were not accepted
were 54 non-commissioned officers and privates of the 1st Louisiana
Native Guards, and 37 of the 3d.  From among the officers of the
general staff and staff departments that were eager to go, two were
selected to accompany the column and keep up the communication with
headquarters and with the other troops; these were Captain Duncan
S. Walker, assistant adjutant-general, and Lieutenant Edmund H.
Russell, of the 9th Pennsylvania Reserves, acting signal officer.

Then the officers and men quietly prepared themselves for the
serious work expected of them.  Those that had any thing to leave
made their wills in the manner sanctioned by the custom of armies,
and all confided to the hands of comrades the last words forto time found themselves
so fortunate as to be able to snatch a few hours' rest in the dense
shade of the splendid forest, until their tour of duty should come
again in the trenches, where, under the June sun beating upon and
baking all three surfaces, the parched clay became like a reverberating
furnace.  The still air was stifling, but the steam from the almost
tropical showers was far worse.  Merely in attempting to traverse
a few yards of this burning zone many of the strongest men were
sunstruck daily.  The labor of the siege, extending over so wide
a front, pressed so severely upon the numbers of the besieging
army, always far too weak for such an undertaking in any climate
at any season, above all in Louisiana in June, that the men were
almost


June 22d, their
dead and wounded were removed under a flag of truce.(1)

While the flag was out, Cahill came up from New Orleans with the
9th Connecticut, a further detachment of the 26th Massachusetts,
and the remainder of Grow's battery.  This gave Stickney about
1,100 men, with four guns in position and six field-pieces.  Cahill's
arrival was seen by Major, who, after waiting all day in a drenching
rain, began to think his condition rather critical; accordingly,
at nine o'clock in the evening he set out to force his way to
Brashear, where he was expecting to find Green.  Riding hard, he
arrived at the east bank of Bayou Boeuf late the next afternoon,
and, crossing by night, at daylight on the 24th he had completely
surrounded the post of Bayou Boeuf, and was just aboutand for three or four hours had been steering
to the eastward, he found himself off the entrance to the Calcasieu,
thirty miles east of the Sabine.  Then he and Weitzel agreed that,
under the circumstances, the best thing to be done was to intercept
the remainder of the expedition, supposed to be following, under
the immediate command of Franklin, and assembling the whole force
where they were to wait until the next morning, the 8th of September,
for the attempt at Sabine Pass.  But the arrangement had been that
the attack by the gunboats to cover Weitzel's landing was to be
made early on the morning of the 7th.  Accordingly Franklin, with
his part of the fleet, carrying the supporting force, had already
passed Berwick Bay; in fact, at eleven o'clock he was off Sabine
Pass;


down
her colors and hoisted the white flag at the fore, and after bravely
continuing the fight for twenty minutes longer the _Clifton_ followed
suit.

The place where the _Clifton_ grounded was fairly in range of the
beach where Weitzel was expected to land his troops.  There may
have been a minute, or even ten, during which it might have been
possible for Weitzel, breaking away from the concerted plan, to
have thrown his picked men ashore while the attention of the
Confederates was fixed upon the _Clifton_; yet, although this
criticism has been suggested by high authority, the point would
have been a fine one at best; and under the actual circumstances,
with the _Granite City_ in the channel ahead, the _Arizona_ aground,
and the guns of the _Sachem_ and the _Clifton_ about to be added
to those with which Bee and Walker had in fact
turned the whole left flank, and were rapidly moving on, breaking
in the line as they advanced.  This soon left Nims's guns without
support, and at the same time Klauss and Cone came under a fire so
severe from Walker's men, that Ransom determined to withdraw to
the cover of the wood in his rear at the edge of the clearing.
Unfortunately, Captain Dickey, his assistant adjutant-general, fell
mortally wounded in the act of communicating these orders, and thus
some of the regiments farther toward the right, being without
orders, and fighting stubbornly against great odds, stood their
ground until they were completely surrounded and taken prisoners.
While aiding Landram to rally and reform the remnants of his division
in the skirt of timber, Ransom was severely wounded in the knee,
and had


use in improvising a bridge of steamboats across the
Atchafalaya.  In his report, Banks speaks of this as the first
attempt of the kind, probably forgetting, since it did not fall
under his personal observation, that when the army moved on Port
Hudson the year before, the last of the troops and trains crossed
the river at the same place in substantially the same way.  However,
the Atchafalaya was then low: it was now swollen to a width of six
hundred or seven hundred yards by the back water from the Mississippi,
and thus the floating bridge, which the year before was made by
lashing together not more than nine boats, with their gangways in
line, connected by means of the gangplanks and rough boards, now
required twenty-two boats to close the gap.  Over this bridge,the James for the surprise and seizure of
Petersburg came to a stand-still on the 18th.  On the 23d Grant
made the request and the orders were issued the next day.

(5) In the official records wrongly printed as the 160th.

CHAPTER XXX.
ON THE POTOMAC.

Grant had meant to send the troops to join the Army of the James
under Butler at Bermuda Hundred, but already the dust of Early's
columns was in sight from the hills behind Washington, and the
capital, though fully fortified, being practically without defenders,
until the Sixth Corps should come to the rescue, in the stress of
the moment the detachments of the Nineteenth Corps were hurried up
the Potomac as fast as the transports entered the roads.  It was
noon on the 11th when Davis landed the fourteen companies from the
_Crescent_ at


Monocacy just in time to enable Wallace, with a chance
medley of garrison and emergency men, to face Early on the 9th,
and compel him to lose a day in crossing.  Then, at last, made
quite certain of Early's true position and plans, Grant hurried
the rest of the Sixth Corps to the relief of Washington, and thus
the steamboat bearing the advance of Wright's men touched the wharf
about two hours after the _Crescent_ had made fast.  The guns of
Fort Stevens were already heard shelling the approaches, and thither
Wright was at once directed, but in the great heat and dust Early
had pressed on so fast that his men arrived before the works parched
with thirst and panting with exhaustion.  Moreover, evening came
before the rear of his column had closed up onand within the next ten days there were assembled there
parts of four brigades--McMillan's and Currie's of the First
division, and Birge's and Molineux's of Grover's.  Part of Currie's
brigade was engaged, under Hancock, in the affair at Deep Bottom
on the north bank of the James on the 25th of July, losing eighteen
killed and wounded and twenty-four prisoners.  The work and duty
in the trenches and on the skirmishing line were hard and constant,
reminding the men of their days and nights before Port Hudson, but
this was not to last long, and the loss was light.(2)

On the 20th of July at Carter's Farm, three miles north of Winchester,
Averell, who was following Early, met and routed Ramseur, who had
been sent back to check the pursuit.  Early continued his retreat
to Strasburg on


as the situation was, the
114th marched steadily forward nearly two hundred yards in front
of the forest; then, finding itself quite alone and unsupported,
confronted by the line of battle of the enemy at the skirt of the
timber opposite, Per Lee made his men lie down without other cover
than the high grass, and there, loading on their backs and at every
moment losing heavily, without yielding an inch, they held off the
enemy until support came.  That this was longer than usual in coming
was no fault of their comrades, but a mere accident of the situation;
for Dwight's division being formed in echelon of battalions on the
right, just as it had in the first instance been necessary to bring
the 114th into action obliquely to the left, so now Beal was forced
to formto General
Keifer for the opportunity to use in this manuscript his paper on
Cedar Creek, prepared for the same series.

(9) Called Mill Creek in Sheridan's report and "Memoirs."  There
is a mill on the north bank.

(10) "Personal Memoirs of P. H. Sheridan," vol. ii., pp. 75-83.
The distance from Winchester to Getty's position is ten and three
quarter miles.

(11) Vol. iv., pp. 524, 532.  And see appendix for the valuable
memorandum kindly prepared expressly for this work by General E.
C. Dawes.

(12) Justly or unjustly; unjustly I think, being unable to see how
any one could have done better.

CHAPTER XXXV.
VICTORY AND HOME.

On the 7th of November, on the battle-field of Cedar Creek, Emory
passed his corps in review before Sheridan.  Sheridan spoke freely
and in the highest terms of the soldierly bearing and good


Battery             Capt. Ormand F. Nims
2d Massachusetts Battery              Capt. Henry A. Durivage (2)
                                      Capt. Jonathan E. Cown

Third Brigade:
  Col. George F. Shepley
12th Maine                            Lt.-Col. W. K. Kimball
13th Maine               1   9     8  48       21    87
  Total Fourth Brigade          3  25    13 137       31   209
Artillery:
1st Maine Battery                   2     1   5              8


     2   3     3  43       22    73
114th New York                  1  20     6  80    1   7   115
116th New York                      7     4  39        9    59
153d New York                 George H. Harris              G
Cpl. Peter A. Martin (3)           G
Cpl. Francis M. Hurd               G
Pvt. George W. Dailey (3)          G
Pvt. Freeman Hadden (3)            G
Pvt. John W. McBride (3)           G
Pvt. Robert Payne (3)              G
Pvt. Charles E. Plummer (3)        G
Pvt. Enoch T. Simpson (3)


Company C

4th WISCONSIN.                     Company
Lt. Isaac N. Earl                  C
Cpl. L. C. Bartlett                C
Pvt. Patrick Pigeon (2)            A

Note.--On the 28th of June, 1863, Birge reported to Headquarters, 2
battalions of stormers, of 8 companies each, present for duty--67
officers, 826 men, total 893.  His duplicate roll, evidently of later
date than June 28th and not later than July 7th, accounts for 10
companies with 71 officers and 865 men, totalwhich didn't allow for my sleep mode to trigger. The problem? the device needed to lay flat to close. Too much material if you ask me and if your like me I don't have time to make sure that my iPad is always flat. So I believe this case needed a clasp to hold it shut. So it missed its mark which is why I gave it two stars.

I just purchase this jean jacket for my iPad (4th generation) today, so I am still formulating my opinion. However, I will say I had been all over town looking for just the right cover and could not be satisfied with the options out there. When the man at the Brookstone store was showing me their suggestions for covers I


use. The only reason I gave this item 4 stars instead of 5 is becauses it's a little bulky and because when you use it in horizontal mode it sits with the button on the left. I'm talking about the little button on the iPad that you push to change screens. In other words it sits backwards from all other cases. It really bugged me at first but now I don't even think about it. Overall I love the case and would highly recommend it.

I thought this was the cutest case. My case was getting old so I decided to replace it with this one. I only had this case a month and I my Ipad and the screen cracked. There are parts of the case that areDetails from Tafheem-ul-Quran

Name

This Surah is called Nun as well as Qulum, the words with which it begins.

Period of Revelation

This too is one of the earliest surahs to be revealed at Makkah, but its subject matter shows that it was sent down at the time when opposition to the Holy Prophet (upon whom be peace) had grown very harsh and tyrannical.

Theme and Subject Matter

It consists of three themes: Replies to the opponents objections, administration of warning and admonition to them, and exhortation to the Holy Prophet (upon whom be peace) to patience and constancy.

At the outset, the Holy Prophet has been addressed, to the effect: "The disbelievers call you a madman whereas the Book that you are presenting and the sublime conduct that you practise, are by themselves sufficient


to refute their false accusations. Soon they will see as to who was mad and who was sane; therefore, do not at all yield to the din of opposition being kicked up against you, for all this is actually meant to cow you and make you resort to a compromise with them."

Then, in order to enlighten the common people the character of a prominent man from among the opponents, whom the people of Makkah fully well recognized, has been presented, without naming him: At that time, the Holy Prophet's pure and sublime conduct was before them, and every discerning eye could also see what sort of character and morals were possessed by the chiefs of Makkah, who were leading the opposition against him.

Then, in vv. 17-33, the parable*/
    public function handle(RequestPasswordReset $command)
    {
        $email = $command->email;

        $validation = $this->validatorFactory->make(
            compact('email'),
            ['email' => 'required|email']
        );

        if ($validation->fails()) {
            throw new ValidationException($validation);
        }

        $user = $this->users->findByEmail($email);

        if (! $user) {


se
Is 11386259 composite?
True
Is 276389 composite?
False
Is 322963462 prime?
False
Is 2432887 prime?
False
Is 912643 prime?
False
Is 51643079 a composite number?
True
Is 1814761 composite?
True
Is 815137487 a composite number?
True
Is 22889303 a prime number?
False
Is 71116049 a prime number?
False
Is 49325285 composite?
True
Is 5193761 prime?
False
Is 4180541 prime?
True
Is 42187199 a composite number?
True
Is 41660839 prime?
False
Is 25128269 composite?
True
Is 102080941 prime?
True
Is 22496447 prime?
True
Is 15363173 composite?
True
Is 21001541 composite?
True
Is 2262679 prime?
True
Is 634562533 prime?
False
Is 225118563 composite?
True
Is 120681511 a prime number?
False
Is 7662199 prime?
False
Is 21209743 a prime number?
True
Is 5073703 prime?
False
Is 546529 prime?
False
Is 40149613 a prime number?
False
Is 22642709 a prime number?
True
Is 1106761 composite?
False
Is 11750807 prime?
True
Is 40591 composite?
False
Is 42723507 a composite number?
True
Is 13374583 composite?
False
Is 2183887 composite?
True
Is 1003001 composite?
False
Is 406189 a composite number?
True
Is 4985537 composite?
True
Is 45029899 composite?
False
Is 109694971 composite?
True
Is 3161519 composite?
False
Is 276005117 prime?
False
Is 9151507 a composite number?
False
Is 1381001 prime?
False
Is 3615691 a prime number?
False
Is 245333153 a prime number?
False
Is 4964237 a composite number?
True
Is 6782851          \usepackage{amsfonts}
                \usepackage{amssymb}
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                \begin{document}$$\theta _{j}^{t+1}$$\end{document}$, the least square loss function is defined as:$$\documentclass[12pt]{minimal}


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                \begin{document}$$\begin{aligned} e(\mathbf{x} ,y)\leftarrow e(\mathbf{x} ,y)+(\theta ^{t+1}-\theta ^{t})h_{\theta }(\mathbf{x} ) \end{aligned}$$\end{document}$$To reduce the complexity of calculating $\documentclass[12pt]{minimal}
                \usepackage{amsmath}
                \usepackage{wasysym}
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                \usepackage{amssymb}
                   \usepackage{amsbsy}
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                \usepackage{upgreek}
                \setlength{\oddsidemargin}{-69pt}
                \begin{document}$$\begin{aligned} q(\mathbf{x} ,f)\leftarrow q(\mathbf{x} ,f)+(\mathbf{V} _{jf}^{t+1}-\mathbf{V} _{jf}^{t})x_{i} \end{aligned}$$\end{document}$$

Distributed BCD Framework {#Sec5}
=========================

In this section, we first introduce how to infer FM by BCD, and then give the details of the propose distributed BCD framework.

Learning FM with BCD {#Sec6}
--------------------

CD updates one coordinate each time while fixing others unchanged. However, if we directly extend it to the distributed platform without any


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                \begin{document}$$\mathbf{V} _{j}$$\end{document}$, i.e., a block of parameters can be $\documentclass[12pt]{minimal}
                \usepackage{amsmath}
                \usepackage{wasysym}
                \usepackage{amsfonts}
              \setlength{\oddsidemargin}{-69pt}
                \begin{document}$$\mathbf{V} $$\end{document}$. Or, put another way, we first update all blocks which include $\documentclass[12pt]{minimal}
                \usepackage{amsmath}
                \usepackage{wasysym}
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different methods respectively.**Parameter Analysis.** We analyze the effect of different factors in our methods: different blocking schemes and using pre-computation or not.**Platform and Implementation.** All methods are implemented using Scala and performed on a cluster containing 16 machines. Among them, 15 machines are used as workers, of which each one contains 2 CPU cores and 16 G memory, and the other one is served as the server, which contains 8 CPU cores and 64 G memory. For the BCD-based algorithms, we default to set the block size as 5000 in Movielens[3](#Fn3){ref-type="fn"} datasets and 10000 in Yahoo music dataset. Due to the different convergence rates of SGD and CD algorithms, we set *T* as 10, 20 and 3000 for BCD-based methods, CD baseline and gradient based algorithms, respectively.

Accuracy and Efficiency Performance \usepackage{amsbsy}
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                \begin{document}$$\times $$\end{document}$ speedup on both Movielens datasets and 7.2$\documentclass[12pt]{minimal}
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                \usepackage{upgreek}
                \setlength{\oddsidemargin}{-69pt}
                \begin{document}$$\begin{aligned} \widehat{y}(\mathbf{x} )= w_{0}+\sum \nolimits _{i=1}^{p}{} \mathbf{w} _{i} \mathbf{x} _{i}. \end{aligned}$$\end{document}$$Compared with FM, LR has fewer features but with similar update rule. Thus, LR can directly apply the distributed BCD framework.

**Matrix Factorization.** MF only incorporates user and item identifications as features and equals to the FM that ignores the other heuristic features, i.e.,$$\documentclass[12pt]{minimal}
              Tutorials

One major down side to using a static site generator like Jekyll is the lack of dynamic updates. CMS tools like Wordpress allow for scheduling posts for the future which is almost essential for consistent blog posts. So what do you do if you want a static site but also want to schedule posts for the future? Well if you are hosting your site on your own sever then you can easily automate your site update process. In this post I’ll be showing you how I schedule posts for my own site. This is a slightly specific usage so your millage my vary but these ideas should be easily adaptable to many situations.

Most games have some sort of feedback system to let the player know what’s happening. These


systems can inform the player if they are on the right path, give context to the story, and give help to the player. Dialogue is a great example of one of these feedback systems. For this tutorial I’m going to be showing you have to create an intractable dialogue system in Unreal Engine 4. You can download it for free on their website. For this project you can checkout the source code on my Github.

In my previous post, Setting Up Xcode and Visual Studio for FMOD Development, I talked about getting your IDE environment ready for creating an FMOD audio engine implementation. In this tutorial I’ll go over creating a basic audio engine that you can use in your C++ projects to add quick and easy dynamic audio.sciences in the MU School of Medicine. "The old depiction of linear evolution from ape to human with single steps in between is proving to be inaccurate. We are finding that evolution seemed to be experimenting with different human physical traits in different species before ending up with Homo sapiens."

Three early species belonging to the genus Homo have been identified prior to modern humans, or Homo sapiens. Homo rudolfensis and Homo habilis were the earliest versions, followed by Homo erectus and then Homo sapiens. Because the oldest erectus fossils that have been found are only 1.8 million years old, and have different bone structure than the new fossil, Ward and her research team conclude that the fossils they have discovered are either rudolfensis or habilis. Ward says these


his plea
of guilty. Specifically relevant to the case sub judice is this Court’s framing of the issue in
Carlos Bryan:

                The state contends that this court does not have jurisdiction to
        entertain the appeal. It contends a certified question of law pursuant to a
        negotiated plea of guilty with an agreed sentence also requires the consent
        of the state under subjection (i) of Tennessee Rule of Criminal Procedure
        37(b(2) and may not be certified pursuant to subsection (iv), the latter of
     cause to believe that they’re intoxicated that you can take blood
        from them and you don’t have to read them the implied consent.

        The blood was drawn by paramedic Jeffrey Strickland, the only other witness who
testified at the suppression hearing. Mr. Strickland testified that Defendant had to be
extricated from his vehicle following the wreck. Although in pain, Defendant remained
conscious and responded to inquiries made by Mr. Strickland. The paramedic smelled
alcohol on Defendant while examining him. Mr. Strickland withdrew the blood sample from
Defendant per Lt. Miller’s request, and the paramedic followed proper procedures in doing
so. Lt. Miller also testified that he never took any steps to request a search warrant to draw
a blood sample from


for appeal. State v. Day, 263 S.W.3d 891, 900
(Tenn. 2008) (“As we have stated repeatedly, no issue beyond the scope of the certified
question will be considered.”); Preston, 759 S.W.2d at 650; Tenn. R. Crim. P. 37(b)(2)(A)(ii)
(“the question of law as stated in the judgment or order reserving the certified question
identified clearly the scope and limits of the legal issue reserved”).

      It is appropriate to quote from a portion of a footnote in our Supreme Court’s opinion
in Day:

       . . . . When crafting a certified question, both the defendant and the State
       would be prudent to review the Rule, craft the certified question to [ensure]
                 -13-
suppression hearing about “the time needed to prepare and present a warrant.” Defendant
takes the position that since there was proof that Officer Miller had the cell phone number
of the General Sessions Court Judge, but still made no attempt to procure a search warrant
before ordering a blood withdrawal from Defendant, that the State thus “failed to carry its
burden as to the existence of exigent circumstances.”

        It is well settled that drawing a person’s blood is considered a seizure, subject to the
provisions of the Fourth Amendment to the United States Constitution, as applicable to the
states through the Fourteenth Amendment, and to the provisions of Article I, § 7 of the
Constitution of Tennessee.


State v. Scarborough, 201 S.W.3d 607, 616 (Tenn. 2006).
Evidence obtained in violation of constitutional provisions prohibiting unreasonable searches
and seizures must generally be suppressed. State v. Yeargan, 958 S.W.2d 626, 629 (Tenn.
1997). A warrantless search and seizure is presumed unreasonable, unless the search and
seizure was conducted pursuant to one of the narrowly defined exceptions to the warrant
requirement. Id.; State v. Carter, 160 S.W.3d 526, 531 (Tenn. 2005).

       One exception to the warrant requirement is a search based upon probable cause and
exigent circumstances. Schmerber v. California, 384 U.S. 757, 770, 86 S. Ct. 1826 (1966).
Regarding the issue of exigent circumstances as it relates to the withdrawal of a blood
sample, this Court in State v. Jordan, 7 S.W.3d 92, 99 (Tenn. Crim. App. 1999) adoptedappeal pertains only to factor (b) above. We take note that
the State argues in its brief that: (1) application of the exclusionary rule in this case would
be inappropriate, even if Defendant’s issue had merit; and (2) Defendant consented to the
withdrawal of his blood due to the lack of any objection to the procedure, and by operation
of his motor vehicle on the public roads of Tennessee. We would not be able to address
these claims by the State because they were not properly included within the certified
question of law. See Day, 263 S.W.3d at 900, n. 8 (quoted above).

        Defendant relies upon two cases in support of his argument regarding the issue which
he attempted to reserve for appeal: Johnson v. United States, 333


input $X=a$ assumed by the receiver, and correspondingly, $\sum_{a\in{\ensuremath{\mathcal{X}}\xspace}}q(a,\cdot)$ plays the role of a channel output statistics assumed by the receiver.

Metric Design: Examples {#sec:metric design}
=======================

By the information inequality , we know that $$\begin{aligned}
  \operatorname{\mathbb{E}}[-\log_2 P_Z(X)]\geq \operatorname{\mathbb{E}}[-\log_2 P_X(X)]=\operatorname{\mathbb{H}}(X)\label{eq:entropy inequality}\end{aligned}$$ with equality if and only if $P_Z=P_X$. We now use this observation to choose optimal metrics.

Mutual Information
------------------

Suppose we have no restriction on the decoding metric $q$. To maximize the achievable rate, we need to minimize the uncertainty in . We have $$\begin{aligned}
\operatorname{\mathbb{E}}\left[-\log_2\frac{q(X,Y)}{\sum_{a\in{\ensuremath{\mathcal{X}}\xspace}}q(a,Y)}\right]&=\operatorname{\mathbb{E}}\left[\operatorname{\mathbb{E}}\left[\left.-\log_2\frac{q(X,Y)}{\sum_{a\in{\ensuremath{\mathcal{X}}\xspace}}q(a,Y)}\right\vert Y\right]\right]\\
&{\overset{\text{(\ref{eq:entropy inequality})}}{\geq}} \operatorname{\mathbb{E}}\left[\operatorname{\mathbb{E}}\left[\left.-\log_2P_{X|Y}(X|Y)\right\vert Y\right]\right]\\
&=\operatorname{\mathbb{H}}(X|Y)\end{aligned}$$ with equality if we use the posterior probability distribution as metric, i.e., $$\begin{aligned}
q(a,b)=P_{X|Y}(a|b),\quad a\in{\ensuremath{\mathcal{X}}\xspace},b\in{\ensuremath{\mathcal{Y}}\xspace}.\end{aligned}$$ Note that this choice of $q$ is not unique, in particular, $q(a,b)=P_{X|Y}(a|b)P_Y(b)$ is also optimal, since the factor $P_Y(b)$ cancels out. For the optimal metric, the achievable rate isQ:

How to load $.ajax (jquery) response to iframe every 20 seconds?

How i can load this jquery get response to middle iframe in my page? The php script loads some data from mysql db? in this case i am not passing any data to php so do i need to make get or post request ? my code display the jquery response in alert but doesn't load it into iframe!
    setInterval(function() {

      $.ajax(
    {
        type: 'GET',
        url: './getDataFromMySQLdb.php',
        //data: $("#myform").serialize(),

         data: {


about what Robert Moses' role was in the way that O'Malley decided to leave Brooklyn? It's hard to imagine one individual being so powerful in one city during one era as Robert Moses was in the 1950s during New York. He was obviously not an elected official, never elected to anything, but because of his administrative positions during the 1950s, Moses controlled what got built in New York, who built it, where it got built.

When Walter O'Malley decided that he wanted to build a new stadium in Brooklyn, one that he would own, he realized very quickly that while he had the finances to build the stadium on his own, he didn't have the finances to get the land. The land would be very expensive and certainly veryhave happened.

Moses had the kind of ego where he just generalizes from his own feelings outward to the situations he encountered. Moses never considered, "Well I might not be a baseball fan but there are millions of them there, and they do have money to spend, and a stadium in downtown Brooklyn could be a very good thing for the borough and generate revenue. Even though I am not a fan, there are many other fans, I'm sure] "I don't think Moses's mind worked that way. I think he thought what he thought and when he thought it, he thought it was right for everyone. O'Malley had a very, very difficult adversary in Moses.

Robert Moses, who escaped blame when the Dodgers left town. (C.M. Stieglitz, World Telegram staff


if he filled his stadium up with all the Hollywood types that he could find, he would only probably sell about 5,000 tickets. There had to be more to it than that. What he was able to very skillfully do was to market the team in two ways at once, as a glamorous sort of place to be, and also as a place that was inexpensive for families.

His ticket structure, $3.50, $2.50, $1.50 for around the first 15 years, well into the '70s, was designed to attract families and repeat viewers. These families would be attracted by the atmosphere of the stadium, of course by the low ticket prices, and also to an extent, by the perhaps opportunity to see a Cary Grant or Frank Sinatra in thedrawing.

I can think of anything to write for this essay im a highschool freshman, my teacher said i can make things up if i want, which i have to cause my.

Check out our top free essays on christmas break essay to help you winter break essay winter break essay if you’re looking for an essay about a thrilling.

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"""
    return separator.join([s for s in self._all_strings(
                strip, types=types)])
getText = get_text

Turns out the separator argument is the reason behind the behavior I encountered...changing my code like so returns the expected results:
In [113]: nav_content.getText(separator=" ")
Out[113]: '\n \n Etusivu Yritys Yritysesittely Kuvagalleria \n Projektiesittely Tuotteet Rakentaminen Mestaripihatto Rakentaminen ja suunnittelu Betonielementit Kattorakenteet Mestarikasvattamo Betoniritilät Pihattokalusteet ja -laitteet GEA lypsy ja maidonkäsittely GEA  pihattokalusteet GEA  ruokinta GEA lannanpoistolaitteet Varaosat ja tarvikkeet Merivirta - kalusteet Nautojen hyvinvointi Karjatalous Ilmanvaihto Irtorehusiilot Valaistus Lietesäiliön verkkoaita Lannankäsittely  Flygt-lietepumput Jälkimarkkinointi Yhteystiedot \n'

Q:

Finding the Binomial Coffecient

Is there a way to find the binomial coefficient of $x^{14}$ in
$$(x^0+x^1+x^2+x^3+x^4)^6$$
I tried to use sum of G.P form but it did'nt help.

A:

HINT:
$$\sum_{r=0}^4 x^r=\frac{1-x^5}{1-x}$$
$$\implies(x^0+x^1+x^2+x^3+x^4)^6=(1-x^5)^6(1-x)^{-6}$$
Now $(1-x^5)^6=1-\binom61x^5+\binom62(x^5)^2-\binom63(x^5)^3+\cdots+x^{30}$
and $(1-x)^{-6}=1+(-6)(x)+\frac{(-6)(-6-1)}{2!}x^2+\frac{(-6)(-6-1)(-6-2)}{3!}x^3+\cdots$


Q:

Does $\lim_{N\rightarrow \infty} \sum_{n = 1}^{N} \frac{1}{(N+1) \ln (N+1) - n \ln n } = 1$?

Question:
Find the limit
\begin{equation}
A = \lim_{N\rightarrow \infty} \sum_{n = 1}^{N} \frac{1}{(N+1) \ln (N+1) - n \ln n }
\end{equation}
The series originated from the asymptotic analysis in this question. I can show that it converges. Numerical evaluation in Mathematica suggests that it is close to $1$.
I am just curious, is it possible to prove one of the following?

$A > 1$
$A = 1$
$A < 1$

Perhaps one can think about the integral
\begin{equation}
\int_0^1 \frac{1}{( 1 + \frac{1}{N} ) \ln (N+1) - x \ln (x N) } dx
\end{equation}
Update 1:
Let $A_N = \lim_{N\rightarrow \infty} \sum_{n = 1}^{N} \frac{1}{(N+1) \ln (N+1) - n \ln n }$, numerical evaluations up to $10,000$ shows $A_N < 1$

We can also plot the
\begin{equation}
  \frac{f( 1 + \frac{1}{N} ) - f(x) }{1 + \frac{1}{N} - x} = f'( y) \le f'( 1 + \frac{1}{N} ) = 1 + \frac{1 + \ln (1 + \frac{1}{N})}{ \ln N }
\end{equation}
Hence
\begin{equation}
 \frac{1}{1 + \frac{1 + \ln (1 + \frac{1}{N})}{ \ln N }} \int_0^1 \frac{1}{1 + \frac{1}{N } - x } dx  \le     I_N  \ln N \le \int_0^1 \frac{1}{1 + \frac{1}{N } - x } dx
\end{equation}
Both integrals on the left and right are $\ln N$. Therefore
\begin{equation}
 \frac{1}{1 + \frac{1 + \ln (1 + \frac{1}{N})}{ \ln N }}  \le I_N \le 1
\end{equation}
Taking the limit $N\rightarrow$, we have $\lim_{N\rightarrow \infty } I_N = 1$ .

A:

You are right: the limit is $1$.
Here it is the full proof:
For all


hour without having to get in the car to seek it out.

Some community associations, like Durbin Crossing in St. Johns, even host "food truck" nights, where a few mobile restaurants converge at the community center so everyone can gather to try new foods and get to know their neighbors. In Durbin Crossing's case, you can head to the South Durbin Amenity Center in St. Johnsfrom 5 to 8 p.m. every Friday.

In addition, food trucks are also popular fixtures at retail store grand openings and at some golf course events.

How to find Jacksonville's food trucks

Because food trucks are on wheels and move about from place to place, you're probably wondering how you can find the one you want to try. The best way is to consult an online portalaluc (a !! 3) (b !! 3) cout2
                     ...

Can anyone suggest a more idiomatic way of representing this? Also, if you feel like browsing the repository, feel free to make any suggestions. I have a bunch of things that I would still like to implement.

A:

Can anyone suggest a more idiomatic way of representing this?

The equations for the ALU have the form
(out !! i, cout !! i) = aluc (a !! i) (b !! i) (cout !! (i - 1))

so we should be able to express all of the equations with a single equation that manipulates lists. If only cout !! (i - 1) would be something !!


(4.14) together with constraint (4.5), we obtain the expressions \[16\] $$M=(+,-,\mu )  \tag{4.15a}$$ $$X^{M}=(1,\frac{x^{2}}{2},x^{\mu })  \tag{4.15b}$$ $$P^{M}=(0,x.p,p^{\mu })  \tag{4.15c}$$ $$A^{M}=(0,x.A,A^{\mu })  \tag{4.15d}$$ In this particular gauge the components of the gauge invariant $L_{MN}^{I}$ become $$L_{+\mu }^{I}=p_{\mu }^{I}=p_{\mu }-A_{\mu }  \tag{4.16a}$$ $$L_{\mu \nu }^{I}=M_{\mu \nu }^{I}=x_{\mu }(p_{\nu }-A_{\nu })-x_{\nu
}(p_{\mu }-A_{\mu })  \tag{4.16b}$$ $$L_{+-}^{I}=D^{I}=x.(p-A)  \tag{4.16c}$$ $$L_{\mu -}^{I}=\frac{1}{2}K_{\mu }^{I}=x_{\mu }x.(p-A)-\frac{1}{2}x^{2}(p_{\mu }-A_{\mu })  \tag{4.16d}$$ The expressions that appear in the right-hand side of equations (4.16) are respectively the extensions of the free conformal generators (2.6) to the case when a background gauge field $A_{\mu }$ is present. It can be verified, using brackets (2.8) and (3.12), that $p_{\mu }^{I}$, $M_{\mu \nu
}^{I}$, $D^{I}$ and $K_{\mu }^{I}$ satisfy the same conformal algebra (2.7) as in the1612 1814 (in the form of family lists), NLI Pos. (microfilm) 5531.

Grange, births, marriages and deaths up to 1859, Library of the Society of Friends, Dublin. Also NLI Pos. 1022.

Lisburn, births, marriages and deaths up to 1859, Library of the Society of Friends, Dublin. Also NLI Pos. 1022.

Limerick, births, marriages and deaths up to 1859, Library of the Society of Friends, Dublin. Also NLI Pos. 1022.

Lurgan, births, marriages and deaths up to 1859, Library of the Society of Friends, Dublin. (NLI Pos. 1022) See also Lurgan Marriage Certificates, Library of the Society of Friends, Lisburn. (NLI Pos. (microfilm) 4126).

Moate, births, marriages and deaths up to 1859, Library of the Society of Friends, Dublin. Also NLI Pos. 1022.

Mountmellick, births, marriages and deaths up to 1859, Library of the Society


The place of proximity: social support in mother-adult daughter relationships.
The mother-adult daughter relationship has been highlighted in both the social sciences and the public health literature as an important facet of social support networks, particularly as they pertain to maternal and child health. Evolutionary anthropologists also have shown positive associations between support from maternal grandmothers and various outcomes related to reproductive success; however, many of these studies rely on proximity as a surrogate measure of support. Here I present data from the Puerto Rican Maternal and Infant Health Survey (PRMIHS) comparing geographic proximity of mother and daughter with a self-reported measure of mother-to-daughter support. These two measures were used to predict infant health outcomes as well as various measures of instrumental and emotional aid provided during pregnancy andunderwater restaurant that the company designed has finally been finished.

The name of the new restaurant is quite simple: Under. It has room to seat 100 guests when it’s at capacity, making it the largest underwater restaurant currently in the world. Not just that, but it’s also the only restaurant of its kind in Europe. The restaurant actually has two functions: to offer diners a unique and once-in-a-lifetime dining experience, as well as serve as a marine life research center.

The building itself is 111 feet long. It sits partially submerged into the ocean, its design lending itself to integrating with the surrounding environment since the building is constructed out of rough concrete with serves as the perfect base for an artificial reef system.

“As a new landmark for Southern Norway,


same time he became the first broadcaster west of the Mississippi to be inducted into the College Football Hall of Fame - broadcaster division. He is also a member of the Pierce County Hall of Fame and during the 2009 season, the Cougars' radio booth in Martin Stadium was renamed the Bob Robertson Broadcast Suite.

Bob's association with WSU runs deeper than just football broadcasts. For 23 years he was the voice of Cougar Basketball, including several NCAA tournament teams. In 1978-79, he was named WSU's Dad of the Year and he also worked with the WSU Cougar Club on the west side in the 1980's.

Among his many accomplishments, Robertson has:

· Spent three decades calling Pacific Coast League baseball in Seattle and Tacoma

· Broadcast professional soccer in Seattle, TacomaIndians simply don’t want a professional police force which can’t be bribed when we’re caught jumping red lights, or our sons drive drunk. We’re just not comfortable with a norm-based criminal justice culture, where you’re judged by what you do, not who you are.

In 1953, India’s first-ever national crime survey had this to say: “Large scale-recruitment during the last few years in all ranks of police has diluted quality to a large extent, and consequently there has been a fall in the standard of work”. “There has”, it went on, “been no improvement in the methods of investigation or the application of science to this work. No facilities exist in any of the rural police stations and even in most of the police stations for scientific work”.

I’ve had


NIA’s budget, but I’m guessing it is nowhere the $8,118 million the FBI received in the 2012 financial year.

Look through estimates for India’s intelligence services, and you’ll see they’re grossly understaffed, too—especially in core areas involving technology and language-specialist skills. The headquarters of the IB’s operations directorate in New Delhi—the cutting edge of the organisation’s counter-jihadist operations—makes do with just 30-odd analysts and field personnel. In 2009, then-Home Minister P. Chidambaram authorised hiring 6,000 new personnel, but the Intelligence Bureau can train only an estimated 600 staff each year—barely covering attrition.

India has a lower ratio of police officers to the general population than almost any country of significance. In 2011, the Union Home Ministry told Parliament it had pushed up the ratio of police officers to the populationto 174:100,000, inching towards the global norm of 250:100,000 or more, and up from a painfully-anaemic 121:100,000. It neglected to note, though, that its claims were based on the 2001 census; adjusted for population growth, the ratio is still an appalling 134:100,000.

3. The third brutal truth is this: the bulk of our police force is made up of not-very-smart people. It is true the average police officer isn’t in the Morgan Stanley race: relatively few people with a choice volunteer for brutal hours and every-day life-threatening risks. However, contrast the New York officer entrance test, and the Delhi Police’s test (which, alas, you’ll have to pay Rs 30 for the privilege of seeing). The first involves some fairly tough tests of comprehension and memory. The second is fairly


assayed for three different types of ROS-related genetic polymorphisms including: regulatory regions of enzymes or processes that generate ROS (myeloperoxidase and tumor necrosis factor-alpha); those that prevent oxidative stress by neutralizing ROS (extracellular and manganese superoxide dismutase, glutathione peroxidase, catalase and glutathione S-transferase Mi); and those that will affect ultimate levels of ROS generated by the metabolism of steroid hormones (catechol O-methyltransferase, glucuronosyltransferase). In addition to the large number of subjects for whom samples are available for the laboratory analyses (n = 1087 cases and 1122 controls), comprehensive assessment of the subjects? environmental exposures has already been obtained in the parent study. Thus, we can also explore whether the genetic polymorphisms in ?at-risk? genotypes will affect associations between breast cancer risk and factors that are likely to becontact case $$\tilde{R}(X,Y)\xi =\tilde{\kappa}\left( \eta \left( Y\right) X-\eta \left(
X\right) Y\right) +\tilde{\mu}(\eta \left( Y\right) \tilde{h}X-\eta \left(
X\right) \tilde{h}Y),  \label{paranullity}$$where $2\tilde{h}:={\mathcal{L}}_{\xi }\tilde{\varphi}$ and, in this case, $%
\tilde{\kappa}=(1-\mu /2)^{2}+\kappa -2$, $\tilde{\mu}=2$.

A $(2n+1)$-dimensional paracontact metric manifold $M(\tilde{\varphi},\xi
,\eta ,\tilde{g})$ whose curvature tensor satisfies (\[paranullity\]), is called *paracontact $(\tilde{\kappa},\tilde{\mu})$-manifold*. The class of paracontact $(\tilde{\kappa},\tilde{\mu})$-manifolds is very large. It contains para-Sasakian manifolds, as well as those paracontact metric manifolds satisfying $\tilde{R}(X,Y)\xi =0$ for all $X,Y\in \Gamma (TM)$ (recently studied in [@ZATZA]). But, unlike in the contact Riemannian case, a paracontact $(\tilde{\kappa},\tilde{\mu})$-manifold such that $%
\tilde{\kappa}=-1$ in general is not para-Sasakian. In fact, there are paracontact $(\tilde{\kappa},\tilde{\mu})$-manifolds such that $\tilde{h}%
^{2}=0$ (which is equivalent to take $\tilde{\kappa}=-1$) but with $\tilde{h}%
\neq 0$. Another important difference with the contact Riemannian case, due to the non-positive definiteness of the metric,


with respect to Riemannian $g$-natural metrics*.* Different. Geom. Appl 2009; 27: 157-169.

Boeckx E. A full classification of contact metric $(\kappa ,\mu
)$-spaces. Illinois J. Math 2000; 44: 212–219.

Calvaruso G. Harmonicity properties of invariant vector fields on three-dimensional Lorentzian Lie groups. J. Geom. Phys 2011; 61: 498-515.

Calvaruso G. Homogeneous paracontact metric three-manifolds*.* Illinois J. Math 2011; 55: 697-718.

Calvaruso G. Harmonicity of vector fields on four-dimensional generalized symmetric spaces. Cent. Eur. J. Math 2012; 10(2): 411-425.

Calvaruso G, Perrone D. H-contact semi-Riemannian manifolds. J. Geom.** **Phys** **2013;71: 11-21.

Calvaruso G, Perrone D, Vanhecke L. Homogeneity on three-dimensional contact metric manifolds, Israel J. Math 1999; 301-321.

Cappelletti Montano B, Di Terlizzi L. Geometric structures associated to a contact metric $(\kappa ,\mu )$-space. Pacific J. Math 2010; 246 no. 2: 257–292.

Cappelletti Montano B, Kupeli Erken I, Murathan C.to place all remaining *Hypocrea* species names, including those with and without recorded *Trichoderma* asexual morphs in *Trichoderma* ([@bax072-B17]). [Figure 1](#bax072-F1){ref-type="fig"} depicts morphology observed in the *Trichoderma*/*Hypocrea* clade.

![Morphology of specimens in the *Trichoderma*/*Hypocrea* clade: **(A)** asexual structures (conidiophore and conidia) of *Trichoderma harzianum* (FJ967806), **(B)** growth in culture of a specimen in the *Trichoderma harzianum* complex and **(C)** sexual reproduction structures of *Hypocrea* species.](bax072f1){#bax072-F1}

Taxonomy of *Trichoderma*
-------------------------

The genus *Trichoderma* has broadly sampled sequence data tied to generally accessible cultures. It contains a number of species with remarkable ecological adaptability that are also important in industrial and biological control applications as well as opportunistic pathogens of humans ([@bax072-B20]). It has also garnered enough research interest (\>15 000 references in Web of Science) to already have rudimentary comparative genomic data available ([@bax072-B21]).

We


RPB2 (NP_014794.3) from the reference strain *S. cerevisiae* S288C as query against the protein sequences obtained in first search was performed and only those hits that met the following criteria were retained: Protein sequence length 200 and longer;blastp alignment start between positions 390 and 590 and end between positions 640 and 840 of the Sce RPB2 protein sequence;more than 40% sequence identity.As a result all accessions contained protein sequence that overlapped by at least 100 amino acids. To find the associated nucleotide accessions, the following ESearch utility was executed in a loop: *epost -db protein -format acc -id \$i \| elink -target nuccore \| efetch -format docsum \| xtract -pattern DocumentSummary -element Caption TaxID Organism ≫ RPB2nuc.txt*

Additional *RPB2* nucleotide records that did not contain annotation but used in ([@bax072-B23]) werein databases at NCBI.](bax072f2){#bax072-F2}

*Trichoderma* records in the ITS RefSeq database
------------------------------------------------

### Sequence quality

Most (96%) of the *Trichoderma* RefSeq ITS sequences contained no ambiguous characters and the small number ([@bax072-B9]) that did, had a maximum of 2 ambiguous characters. No chimeras were found compared against the UNITE library or the RefSeq library. Using ITSx annotation predictions ([@bax072-B27]), the complete ITS1 intergenic spacer ranged from 180 to 264 bases, the 5.8S region 158--159 bases and the complete ITS2 intergenic spacer 163 to 199 bases ([Figure 3](#bax072-F3){ref-type="fig"}). Generally, ITS1 was longer than the ITS2, with more length and sequence variation as has been reported previously for other members of the Ascomycota ([@bax072-B28]). Currently, only 10 records have an ITS1 shorter than the minimum (180) as calculated from complete regions and only 5


to a *Penicillium* species instead (JNNP01000511.1, range: 1-1206). *RPB2* sequences within genomes matched to *RPB2* sequences of type material as expected, except in cases were the genome assemblies have likely been identified incorrectly ([Figure 5](#bax072-F5){ref-type="fig"} with matrix and tree provided as [Supplementary Material](#sup1){ref-type="supplementary-material"}) ([@bax072-B17], [@bax072-B24]). The current rate at which fungal genomes from type material are sequenced, assembled and submitted to NCBI is more or less the same rate at which incorrectly labeled genome assemblies are being submitted to NCBI. Therefore, it will be useful to transition to alternative markers such as single copy protein coding genes for genome verification and inclusion into RefSeq in the future.

![A phylogenetic tree generated by a FastTree analysis using a MAFFT alignment of *RPB2* nucleotide sequences from type material (with asterisk) ofvary depending on thin paper with weak stiffness, thick paper, and large- or small-sized paper. However since no consideration is given to the stacking positions corresponding to the types of paper, there is a problem in that paper with a poor stacking characteristic is generated.
Since there are many cases where the discharged paper tray is provided on an upper side of the device in conjunction with the trend toward a large-sized device, and an extension is extended to stack a large-sized medium, there is a problem in that the paper is very difficult to remove.
In the device which has the rejecting function for a case in which a fault has occurred in reading, recognition, and the like, the structure is adopted in which a paper transporting passage separate


from a normal transport passage is generally provided for rejection to discharge the rejected sheet onto a separate tray. However, since the separate transporting passage is provided, there is a problem in that the device becomes large in size.([Figure 6f](#fig6){ref-type="fig"} left and [Supplementary Figure 3e](#sup1){ref-type="supplementary-material"}). Importantly, these differences between various treatments were abolished after NKG2Ds in NK cells was blocked with an anti-NKG2D mAb ([Figure 6f](#fig6){ref-type="fig"} right). Thus, by increasing NKG2DLs expression on BC cells, low concentrations of HDACis sensitized BC cells to NK cell-mediated cytotoxicity.

Discussion
==========

Previous studies have revealed that the immune system influences tumor development and clinical outcomes. NKG2DLs have an important role in cancer immunosurveillance and have clinicopathological significance.^[@bib31],\ [@bib32],\ [@bib33],\ [@bib34],\ [@bib35]^ In BC, however, conflicting results have been reported. Some researchers implied that high expression of MICA/B resulted in a favorable outcome concerning the relapse-free period in early BC patients.^[@bib35]^ However, higher MICA expression was found as an indicator of poor prognosis in BC.^[@bib36]^ In addition, most previous studies were focused on


Institute 1640 medium (Gibco, 31800105, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS; Biological Industries, 04-0101-1, Cromwell, CT, USA). MDA-MB-231 and MDA-MB-468 cells were cultured in Leibovitz\'s L-15 medium (Gibco, 11415114) with 10% FBS. Hs 578T cells were cultured in Dulbecco\'s modified Eagle\'s medium (DMEM; Gibco, 12430047) supplemented with 0.01 mg/ml bovine insulin (Solarbio, I8040, Beijing, China) and 10% FBS. SK-BR-3 cells were cultured in McCoy\'s 5A medium (modified, Gibco, 16600082) with 10% FBS. Hs 578Bst cells were cultured in DMEM with 50 ng/ml epidermal growth factor (Gibco, PHG0311) and 15% FBS. HBL-100 cells were cultured in DMEM with 10% FBS. The cell culture medium was changed every 2--3 days, and the cells were passaged with 0.25% trypsin-EDTA (Gibco, 25200056) and grown to 90% confluence. The cultures were keptat 37 °C with 5% CO~2~ in a water-jacketed incubator (Thermo Scientific, Waltham, MA, USA).

TCGA database
-------------

TCGA is available from the website of the Cancer Genomics Browser of the University of California, Santa Cruz (<https://genome-cancer.ucsc.edu/>). MICA and MICB mRNA expression and miRNA-sequencing level 3 data in BC patients were extracted from TCGA\'s data portal. Only patients with fully characterized tumors, TNM stage, overall survival, complete miRNA information were included. In total, data on 1096 BC patients with detailed miR-20a, MICA and MICB expression were collected from TCGA\'s data portal. Read counts for both mRNAs and miRNAs were used as input for survival analysis with the R package edgeR.^[@bib46],\ [@bib47]^ Kaplan--Meier plots for MICA or MICB expression in association with overall survival were calculated with the R program. Patients were split


surgery. Intraocular pressure was measured on each visit. The findings of post-operative corneal edema and visual acuity between the diabetic and non-diabetic groups were studied and compared. There was no difference clinically in any pre-operative corneal examination between the diabetic and non-diabetic groups. The corneal edema after surgery was significantly higher in the diabetic group than in the non-diabetic group (p less than 0.001). The number of patients with corneal edema one day and one week after surgery was significantly higher in the diabetic group than in the non-diabetic group (after 1 day, OR = 62.5; 95 % CI = 15.31 - 255.11, p less than 0.000) and after 1 week, OR = 6.77; 95 % CI = 1.28 - 35.76, p less than 0.006). Corneal edema followingprophylactic and therapeutic usage of vaccines against viral infections and tumors, the powerful capacity of ONs in enabling and tailoring the immunologic outcome of vaccination will be explored;this proposal is focused on an efficient delivery system that can put ONs and protein antigens into the appropriate subcellular compartments of desired cell types. To test the delivery capabilities of the proposed delivery systems, we will first examine the hypothesis that endosome-permeabilizing listeriolysin O (LLO) co-encapsulated with immunostimulating sequence ONs (ISS-ONs) inside antigen-carrying delivery systems can deliver a significant extent of antigen to the cytosol (i.e., MHCI pathway of antigen presentation) as well as MHCII compartments while enhancing the delivery of ISS-ONs to the cognate receptors in the endocytic vesicles. The antigen- and ISS-ON-carrying LLO-liposomes will be used to test


768 F.Supp. 1150 (1991)
SEAL AND COMPANY, INC., Plaintiff,
v.
WASHINGTON METROPOLITAN AREA TRANSIT AUTHORITY and Dynatran, A Division of Dynalectric Company, Defendants.
Civ. A. No. 91-682-A.
United States District Court, E.D. Virginia, Alexandria Division.
July 8, 1991.
*1151 Paul Terrence O'Grady, Falls Church, Va., for Seal and Co., Inc.
Thomas Bentley Dorrier, Associate Gen. Counsel, Washington, D.C., for Washington Metropolitan Area Transit Authority.
Douglas L. Patin and Frank Filiatreau, Kilcullen, Wilson & Kilcullen, Washington, D.C., for Dynatran, Div. of Dynalectric Co.

MEMORANDUM OPINION
ELLIS, District Judge.

Introduction
This is a disappointed bidder's challenge to a contract award made by the Washington Metropolitan Area Transit Authority ("WMATA"). It raises the infrequently litigated issue of whether an unsuccessful bidder has standing to challenge the contract award of an interstate agency created by interstate compact. Plaintiff, the unsuccessful bidder, charges that WMATA's awardbusiness in Chantilly, Virginia. It is engaged in the electrical contracting business and has successfully bid on and performed contract work for WMATA since 1972. Defendant WMATA is an interstate agency created by the Washington Metropolitan Area Transit Authority Compact ("the Compact") executed by Virginia, Maryland and the District of Columbia and approved by Congress[2] in accordance with the U.S. Const. art. I, § 10, cl. 3. WMATA, under the Compact, is charged with operating the Metro system serving the Washington metropolitan area. Defendant Dynatran is a corporation having its principal place of business in Sterling, Virginia. It is the successful bidder for the challenged contract award.
On February 19, 1991, WMATA issued Invitation for Bids No. IFB-C-882 ("the IFB") requesting bids on the construction of the communications system


in the House Report, further supports the conclusion that Congress intended WMATA to function as a federal agency with respect to procurements. This is also reflected in WMATA's extensive procurement regulations, which state that they
implement pertinent provisions of the Washington Metropolitan Area Transit Authority Compact; OMB Circular A-102 ... UMTA Circular C 4220.1, dated August 6, 1979 and relevant decisions of the courts and the Comptroller General of the United States.
Moreover, the existence of a cause of action for disappointed bidders is arguably implicit in Section 73 and in the scheme of the Compact, for if private bidders on WMATA's contracts lacked standing to challenge WMATA's awards, it is hard to see how Section 73's mandate that contracts be awarded to the lowest responsible bidder would be monitored1990, 90-1 CPD ¶ 287. Put another way:
... a bid should not be rejected as nonresponsive because it does not contain all of the information necessary to perform a Buy American Act analysis only where the additional information required from the bidder would not change the relative standing of the bidders ... or allow the bidder to manipulate its bid to its advantage.

Id.
It is clear from Manatts and other Comptroller General decisions[19] that the language of a specific Buy American Certificate, and the IFB containing it, must be examined to determine whether failure to complete the certificate permits material alteration of a bid subsequent to the opening of bids and hence renders a bid nonresponsive. In this case, the Buy American Certificate really contained two certificates: one for


Buy American Act and UMTA regulations permit the use of foreign sources if a bidder qualifies for an exception. Here, plaintiff simply failed to note whether it intended to provide only domestic products or to seek an exception. Finally, plaintiff argues that since it supplied WMATA with a properly completed Buy American Certificate shortly after bid opening, the contracting officer acted arbitrarily in rejecting its bid. However, "only material available at bid opening may be considered in making a responsiveness determination." Bulloch International, Inc., B-237369, February 5, 1990, 90-1 CPD ¶ 153; accord Rocco Indus., Inc., B-227636, July 24 1987, 87-2 CPD ¶ 87.[23] There is, therefore, no basis for concluding that the contracting officer acted arbitrarily in choosing to ignore plaintiff's after-bid submission. In sum, an examinationCorp., B-209214, Feb. 28, 1983, 83-1 CPD ¶ 197; Avantek, B-170498, March 30, 1971, 50 Comp.Gen. 697, each finding that Buy American Certificate irregularities did not render bid nonresponsive.
[20]  The bid of a bidder who checks the foreign source certification will not be rejected if the bidder qualifies for an exception to the domestic content and assembly requirements of the Buy American Act. An exception to these requirements is permitted where "the inclusion of domestic materials will increase the cost of the overall project contract by more than 10 percent in the case of projects for the acquisition of buses, rolling stock ... and train control equipment, communication equipment, and traction power equipment." 49 C.F.R. § 661.7(d) (1990). Thus, when confronted with a bidder who checks the


Act pertaining to train control and communication equipment, the equipment at issue here, were reprinted in the IFB at pages I-45 and I-46. Moreover, on pages I-46 and BF-6 of the IFB, the UMTA regulations at 49 C.F.R. Part 661 were explicitly referenced. In particular, 49 C.F.R. § 661.13(b), which requires that the Buy American Certificate be included in any IFB as a condition of responsiveness, is referenced on page BF-6 in the first paragraph of the Buy American Certificate included in the IFB.
[25]  The regulation states in relevant part:

(i) Communications equipment includes, but is not limited to, the following equipment:
....
(10) Communication console at central control.
....
(13) Data transmission system central processors.
(14) Data transmission system remote terminals.
....
(17) Security console at central control.
49 C.F.R. § 661.11(i)(10), (13), (14), (17)By the way Assemblyman Carl Heastie was re-elected Democratic County Leader. You can go to my blog to see photos of this event.

The Castle Hill Community Fair took place this past Saturday, with help from State Senators Ruben Diaz Sr. and Jeff Klein along with soon to be Assemblyman Luis Sepulveda. Hundreds of people were in attendance, and while Senator Diaz was still recovering from knee surgery District Leader Julia Rodriguez stood in for Diaz Sr. During redistricting Senator Klein picked up a portion of the area and was out to meet some of his new constituents. Again you can go to my blog to see photos of this event, just check the archive section on the left.

Thursday October 11th will be the 5th Annual Liberty Democratic Association


---
abstract: 'In this paper, a novel and generic multi-objective design paradigm is proposed which utilizes quantum-behaved PSO(QPSO) for deciding the optimal configuration of the LQR controller for a given problem considering a set of competing objectives. There are three main contributions introduced in this paper as follows. (1) The standard QPSO algorithm is reinforced with an informed initialization scheme based on the simulated annealing algorithm and Gaussian neighborhood selection mechanism. (2) It is also augmented with a local search strategy which integrates the advantages of memetic algorithm into conventional QPSO. (3) An aggregated dynamic weighting criterion is introduced that dynamically combines the soft and hard constraints with control objectives to provide the designer with a set of Pareto optimal solutions and lets her to decide the target solutionQPSO algorithm is summarized in Algorithm \[alg:1\].

$|T|$ and $|S|$ denote the number of iterations and swarm size,respectively. $U[0,1]$ is a uniform random number in the range of $[0,1]$ and $f(x)$ is an arbitrary objective function. The QPSO algorithm has some advantages over other variations of the PSO algorithm. It is less sensitive to the bias problem as it has only one parameter to be tuned. It is theoretically guaranteed that the QPSO converges to the global optimum. It is also experimentally shown that the QPSO algorithm outperforms other PSO variations in finding the optimal solutions and has stronger exploring capabilities [@30-sun2012quantum]. For more comprehensive evaluations on the QPSO algorithm, please refer to [@30-sun2012quantum]. The QPSO algorithm has been successfully applied to a vast variety of engineering problems such


It was only equipped with twin engined medium bombers that would very soon be regarded as obsolete. Yet, with fear of an invasion of Britain at its height, RAF Bomber Command was given a task regarded as at least as important as defending the skies above Britain – it was sent out, night after night, to do as much damage as possible to the naval forces that Hitler had gathered for the cross channel attack.

Most of these targets were very well defended. There was no shortage of bravery amongst the aircrew that had to face them.

Sergeant John Hannah was 18 when he became one of the youngest recipients of the Victoria Cross. The original recommendation for the award reads:

On the night of September 15/16th., Sergeant Hannah was theDuring this time, ammunition from the gunner’s magazines was exploding in all directions. In spite of this and the fact that he was almost blinded by the intense heat and fumes, he succeeded in controlling and eventually putting out the fire. During the process of fighting the flames, he had turned on his oxygen to assist him in his efforts. On instructions from his pilot, Sergeant Hannah then crawled forward to ascertain if the navigator was alright, only to find that he also was missing. He informed his pilot and passed up the navigator’s log and maps, stating that he was quite alright himself, in spite of burns and exhaustion from the heat and fumes. An inspection of the aircraft reveals the conditions under which Sergeant Hannah was


The football game played Saturday in Madison could have taken place 20 years ago. Wisconsin performed a complete takedown of Michigan, outclassing the Wolverines on both lines. The Badgers were unstoppable in the running game, piling up 359 yards on 57 carries. They pressured Michigan on 39.6 percent of dropbacks, negating the Wolverines’ speed at the skill positions. They led 28-0 at halftime and had the ball for more than 41 out of 60 minutes.

In short, they played the game that Michigan coach Jim Harbaugh has always wanted his team to play. After he accepted his first head coaching job, at San Diego in 2004, Harbaugh told legendary Michigan coach Bo Schembechler that he would always have a fullback on his roster. As they shift toward spread conceptsis therefore common that the required spring force amounts to a few to ten-odd tons.
The structure disclosed in JP 08-117985A distorts the springs and thus applies pressure to the plate bricks by making a surface pressure bar and a roller engage with each other.
The structure disclosed in JP 2003-200256A relieves the surface pressure with a large-diameter roller which is attached to a surface pressure control bar and climbs over a mountain-like tapered portion formed on the top surface of a surface pressure releasing bar, thus depressing the surface pressure releasing bar and distorting the springs.
The structure of JP 08-117985A, the surface pressure bar and the roller are engaged with each other to distort the springs and apply pressure to the plate bricks, cannot avoid insufficient spring distortion which


class of the projective representation $$p_i:H_i=(H_1)^i\xrightarrow{p_1\times\cdots\times p_1}(PGL_p(\mathbb{C}))^i\rightarrow PGL_{p^i},$$ where the final arrow is given by embedding $PGL_p(\mathbb{C})^i$ into $PGL_{p^i}$ via block matrices down the diagonal. Then, it is not hard to show that the index of $\alpha_i$ is $p^i$. Recall that the index of a class of $Br(G)$ is the least common divisor of all projective representations having that class.
Now, define $G=*_i H_i$, the free product of the $H_i$ for all positive integers $i$. The corresponding classifying space $BG$ is the wedge sum of the $BH_i$. Therefore, the collection of continuous pointed maps
$$\alpha_i:BH_i\rightarrow K(\mathbb{C}^*,2)$$
induces a continuous pointed map
$$\alpha:BG\rightarrow K(\mathbb{C}^*,2)
$$such that the composition $$BH_i\rightarrow BG\rightarrow K(\mathbb{C}^*,2)$$ is $\alpha_i$.
It is easy to see that $ind(\alpha_i)$ divides $ind(\alpha)$ for all $i$. But, since $ind(\alpha_i)=p^i$, it follows that $ind(\alpha)$ is infinite, andQ:

Reflect point over a line in 3D

I'd like to sample points on a triangle randomly. The following code yields points that are uniformly distributed on a quadrilateral:

point = RandomReal[]*(v2-v1) + RandomReal[]*(v3-v1);

I'd like to take advantage of the symmetry and transform points chosen outside the triangle to their reflected position inside the triangle. It seems like this should be simple with ReflectionTransform, but so far, no dice. Here's some code to demonstrate:
Module[{vertices = {{0, 0, 0}, {1, 0, 0}, {0, 1, 1}}, p, r},

 p = (RandomReal[] vertices[[2]] - vertices[[1]]) +
     (RandomReal[] vertices[[3]] - vertices[[1]]);

 r = ReflectionTransform[p];

 Print["p=", p]; Print["r[p]=", r[p]];

 Show[Graphics3D[{Red, Triangle[vertices]}],
      Graphics3D[{Blue, PointSize[0.02], Point[vertices], Green,


observed in neurodegenerative diseases such as Alzheimer's and Parkinson's disease.

Abstract

Cathelicidins are phylogenetically ancient, pleiotropic host defense peptides-also called antimicrobial peptides (AMPs)-expressed in numerous life forms for innate immunity. Since even the jawless hagfish expresses cathelicidins, these genetically encoded host defense peptides are at least 400 million years old. More recently, cathelicidins with varying antipathogenic activities and cytotoxicities were discovered in the venoms of poisonous snakes; for these creatures, cathelicidins may also serve as weapons against prey and predators, as well as for innate immunity. We report herein the expression of orthologous cathelicidin genes in the venoms of four different South American pit vipers (Bothrops atrox, Bothrops lutzi, Crotalus durissus terrificus, and Lachesis muta rhombeata)-distant relatives of Asian cobras and kraits, previously shown to express cathelicidins-and an elapid, Pseudonajaa class of proteins that contains short collagenous domains, along with surfactant proteins A and D, and complement protein C1q. This class of biomacromolecules requires post-translational modifications to form biologically active assemblies. By introducing a set of post-translational modifying enzymes into Escherichia coli , we have created a prokaryotic expression system that functionally assembles adiponectin, as assessed by the ability of produced adiponectin multimers to suppress human endothelial cell apoptosis. This study represents the first example of the assembly of functional high order multimers of any member of this class of proteins outside of eukaryotic cells. Furthermore, the results give fundamental insight into the process of assembly such as the necessity and sufficiency of various post-translational steps for functional assembly. We expect that fine-tuning of the expression system


in combination with a peptoid drag-tag and different fluorophores enabled precise separation of short DNA fragments to establish a new mobility shift assay for detection of primer-dimer formation.

Abstract

Free-solution conjugate electrophoresis (FSCE) is a method of DNA sequencing that eliminates the need for viscous polymer solutions by tethering a carefully designed, mobility modifying "drag-tag" to each DNA molecule to achieve size-based separations of DNA. The most successful drag-tags to date are genetically engineered, highly repetitive polypeptides ("protein polymers") that are designed to be large, water-soluble, and completely monodisperse. Positively charged arginines were deliberately introduced at regular intervals into the amino acid sequence to increase the hydrodynamic drag without increasing drag-tag length. Additionally, a one-step purification method that combines affinity chromatography and on-column tag cleavage was devised to achieve thedays. To measure bioactivity we used Rat L6 myofibroblasts, stimulated them with this slow release insulin and determined activation of the receptors on the cell surface by quantifying AKT phosphorylation. There was only a minor and gradual decrease in AKT phosphorylation over time. To determine whether the slow release insulin could stimulate keratinocyte migration, wounding was simulated by scratching confluent cultures of human keratinocytes (HaCaT). Coverage of the scratch "wounds" was significantly faster in the presence of insulin released from microspheres than in the insulin-free control. Extended and sustained topical delivery of active insulin from a stable protein crystal-based reservoir shows promise in promoting tissue healing.

Abstract

Biomaterials that mimic the extracellular matrix in both modularity and crosslinking chemistry have the potential to recapitulate the instructive signals that ultimately control


formed the homodisulfide, providing a convenient route to prepare peptoid helix homodimers.

Abstract

Interactions with bacterial membranes are integral to the mechanisms of action of all antimicrobial peptides (AMPs), regardless of their final cellular targets. Here, we describe in detail two biophysical techniques that can be used to measure the membrane activities of AMPs and antimicrobial peptidomimetics: (1) a calcein leakage assay to investigate interactions between AMPs/peptidomimetics with large unilamellar vesicles and (2) a potential-sensitive dye-based depolarization assay to investigate interactions with the membranes of live bacteria. By comparing the membrane interactions of AMPs and their mimics, these techniques can provide insights into their extent of mimicry and their antimicrobial mechanisms.

Abstract

The chemoselective glycosylation of N-alkylaminooxy side chains with unprotected reducing sugars has proven useful for the synthesis of glycopeptides. Herein,technologies to flower again. Microchannel electrophoresis, after a quiet period of metamorphosis, may emerge sleeker and more powerful, to claim its own important niche applications.

Abstract

Numerous strategies exist to prevent biological fouling of surfaces in physiological environments; our strategy focuses on the modification of surfaces with poly-N-substituted glycine oligomers (polypeptoids). We previously reported the synthesis and characterization of three novel polypeptoid polymers that can be used to modify titanium oxide surfaces, rendering the surfaces resistant to adsorption of proteins, to adhesion of mammalian and bacterial cells and to degradation by common protease enzymes. In this study, we investigated the effect of polypeptoid chain length on the antifouling properties of the modified surfaces. For these experiments we used poly(N-methoxyethyl) glycines with lengths between ten and fifty repeat units and determined


of two different average molar mass polymers on the DNA sequencing ability of poly(N,N-dimethylacrylamide) (pDMA) sequencing matrices in microfluidic chips. Two polydisperse samples of pDMA, with weight-average molar masses of 3.5 MDa and 770 kDa, were mixed at various fractional concentrations while maintaining the overall polymer concentration at 5% w/v. We show that although the separation of short DNA fragments depends strongly on the overall solution concentration of the polymer, inclusion of the high-molar mass polymer is essential to achieve read lengths of interest (>400 bases) for many sequencing applications. Our results also show that one of the blended matrices, comprised of 3% 3.5 MDa pDMA and 2% 770 kDa pDMA, yields similar sequencing read lengths (>520 bases on average) to the high-molar mass matrix alone, while alsoonto a set of four LDR primers designed to probe the K-ras oncogene for mutations highly associated with colorectal cancer, the simultaneous generation of fluorescently labeled LDR/drag-tag conjugate (LDR-dt) products in a multiplexed, single-tube format with mutant:WT ratios as low as 1:100, respectively, and the single-base, high-resolution separation of all four LDR-dt products. Separations were conducted in free solution with no polymer network using both a commercial capillary array electrophoresis (CAE) system and a PMMA microchip replicated via hot-embossing with only a Tris-based running buffer containing additives to suppress the EOF. Typical analysis times for LDR-dt were 11 min using the CAE system and as low as 85 s for the PMMA microchips. With resolution comparable to traditional gel-based CAE, FSCE along with microchip electrophoresis decreased the separation


pexiganan. Broad-spectrum activity against six clinically relevant BSL2 pathogens is also shown. This comprehensive structure-activity relationship study, including circular dichroism spectroscopy, minimum inhibitory concentration assays, hemolysis and mammalian cell toxicity studies, and specular x-ray reflectivity measurements shows that the in vitro activities of ampetoids are strikingly similar to those of AMPs themselves, suggesting a strong mechanistic analogy. The ampetoids' antibacterial activity, coupled with their low cytotoxicity against mammalian cells, make them a promising class of antimicrobials for biomedical applications. Peptoids are biostable, with a protease-resistant N-substituted glycine backbone, and their sequences are highly tunable, because an extensive diversity of side chains can be incorporated via facile solid-phase synthesis. Our findings add to the growing evidence that nonnatural foldamers will emerge as an important class of therapeutics.

Abstract

The hydrophobic proteinsSP-C mimics: those having a rigid alpha-chiral aromatic helix and those having a biomimetic alpha-chiral aliphatic helix. The length of the two classes of mimics was also systematically altered. Circular dichroism spectroscopy gave evidence that all of the peptoid-based mimics studied here emulated SP-C's secondary structure, forming stable helical structures in solution. Langmuir-Wilhelmy surface balance, fluorescence microscopy, and pulsating bubble surfactometry experiments provide evidence that the aromatic-based SP-C peptoid mimics, in conjunction with a synthetic lipid mixture, have superior surface activity and biomimetic film morphology in comparison to the aliphatic-based mimics and that there is an increase in surface activity corresponding to increasing helical length.

Abstract

To realize the immense potential of large-scale genomic sequencing after the completion of the second human genome (Venter's), the costs for the complete sequencing


on the polypeptoid-modified surfaces compared to unmodified TiO(2) surfaces. The results reveal the influence of polypeptoid side-chain chemistry on short-term and long-term protein, cell and bacterial fouling resistance.

Abstract

Surface modification techniques that create surfaces capable of killing adherent bacteria are promising solutions to infections associated with implantable medical devices. Antimicrobial (AM) peptoid oligomers (ampetoids) that were designed to mimic helical AM peptides were synthesised with a peptoid spacer chain to allow mobility and an adhesive peptide moiety for easy and robust immobilisation onto substrata. TiO(2) substrata were modified with the ampetoids and subsequently backfilled with an antifouling (AF) polypeptoid polymer in order to create polymer surface coatings composed of both AM (active) and AF (passive) peptoid functionalities. Confocal microscopy images showed that the membranes of adherent E. coli cellsthe peptide's proper function. Although the peptoid analogues of SP-B(1-25) studied here share many similar features and all functionally mimic SP-B(1-25) to some degree, it is notable that small differences in their sequences and side chain chemistries lead to substantial differences in their observed interactions with a lipid film. A peptoid comprising a hydrophobic, helical insertion region with aromatic side chains shows more biomimetic surface activity than simpler peptoids, and even better activity, by comparison to natural LS, than SP-B(1-25). However, the substitution of lysine-like side chains for arginine-like side chains in the peptoid has little effect on biomimetic surface activity, indicating that interactions of the guanidino groups with lipids may not be critical for the function of these SP-B mimics.

Abstract

With the complete sequencing of the human genome,


sensitivity relative to equivalent separations that are obtained with zone electrophoresis alone. Even with very long initial sample plugs (up to 3000 microm), this strategy produces electrophoretic separations with high resolutions and peak efficiencies. This strategy can be implemented to increase detection sensitivity in any standard microfluidic electrophoresis platform and does not require any specialized hardware or microchannel configurations.

Abstract

We present the polymer poly-N-hydroxyethylacrylamide (PHEA) (polyDuramide) as a novel, hydrophilic, adsorbed capillary coating for electrophoretic protein analysis. Preparation of the PHEA coating requires a simple and fast (30 min) protocol that can be easily automated in capillary electrophoresis instruments. Over the pH range of 3-8.4, the PHEA coating is shown to reduce electroosmotic flow (EOF) by about 2 orders of magnitude compared to the bare silica capillary. In aa 10 mM Tris-borate-EDTA (TBE) buffer, 0.25 micro g antibody/1 million bacteria, and capillaries dynamically coated with poly-N-hydroxyethylacrylamide (polyDuramide). We have also successfully detected the presence of E. coli O157:H7 in contaminated meat. The total time required for analysis was 6-8 h, which is less than that realized in most commercial assays presently available.

Abstract

The necessity for microchannel wall coatings in capillary and chip-based electrophoretic analysis of biomolecules is well understood. The regulation or elimination of EOF and the prevention of analyte adsorption is essential for the rapid, efficient separation of proteins and DNA within microchannels. Microchannel wall coatings and other wall modifications are especially critical for protein separations, both in fused-silica capillaries, and in glass or polymeric microfluidic devices. In this review, we present a discussion of recent


based on the novel monomer N-hydroxyethylacrylamide (HEA), has been synthesized for application in DNA separation by microchannel electrophoresis. The monomer was found by micellar electrokinetic chromatography analysis of monomer partitioning between water and 1-octanol to be more hydrophilic than acrylamide and N,N-dimethylacrylamide. Polymers were synthesized by free radical polymerization in aqueous solution. The weight-average molar mass of purified polymer was characterized by tandem gel permeation chromatography-multiangle laser light scattering. The steady-shear rheological behavior of the novel DNA sequencing matrix was also characterized, and it was found that the viscosity of the novel matrix decreases by more than 2 orders of magnitude as the shear rate is increased from 0.1 to 1000 s(-1). Moreover, in the shear-thinning region, the rate of change of matrix viscosity with shear rate increases3.4-, 5-, and 20-kDa nominal average molar mass were analyzed by FSCE and MALDI-TOF; the values of the molar mass averages, Mw and Mn, typically agree to within 5%. Measurements and molar mass calculations are performed without any internal standards or calibration. Moreover, when DNA-polymer conjugate analysis is performed in a chip-based electrophoresis system, separation is complete in less than 13 min. FSCE offers an alternative to MALDI-TOF for the characterization of uncharged, water-soluble polymers that can be uniquely conjugated to DNA.

Abstract

Oligomeric N-substituted glycines or "peptoids" with alpha-chiral, aromatic side chains can adopt stable helices in organic or aqueous solution, despite their lack of backbone chirality and their inability to form intrachain hydrogen bonds. Helical ordering appears to be stabilized by avoidance of steric clash as well as


length of about 13 residues for the formation of stable poly(Nrpe) helices. Moreover, no dependence of circular dichroism on concentration is observed for an Nrpe hexamer, providing evidence that these helices remain monomeric in solution. In light of these new data, we discuss chain length-related factors that stabilize organosoluble peptoid helices of this class, which are important for the design of helical, biomimetic peptoids sharing this structural motif.

Abstract

To elucidate the impact of matrix chemical and physical properties on DNA sequencing separations by capillary electrophoresis (CE), we have synthesized, characterized and tested a controlled set of different polymer formulations for this application. Homopolymers of acrylamide and N,N-dimethylacrylamide (DMA) and copolymers of DMA and N,N-diethylacrylamide (DEA) were synthesized by free radical polymerization and purified. Polymer molar mass distributions were characterizedpore-based models of DNA electrophoresis do not apply. We propose a transient entanglement coupling mechanism for the electrophoretic separation of DNA in uncrosslinked polymer solutions, which is based on physical polymer/DNA interactions.

Abstract

We demonstrate the feasibility of temperature-sensitive polymers as novel matrices for capillary and slab electrophoresis of DNA. These matrices combine the case-of-use of agarose with resolution properties of polyacrylamide. Two classes of matrices are used: (i) aqueous suspensions of gel microspheres and (ii) solutions containing uncross-linked temperature-sensitive polymers. When heated, the viscosity of these solutions drops dramatically because of the phase transition behavior of these polymers, and therefore these formulations are easy to pour or load. Results are presented for separation of double-stranded DNA fragments (< 2000 bp) in capillary, tube, and slab electrophoresis. Preliminary results are


If a gene\'s promoter were a light switch, you\'d probably call an electrician. That\'s because rather than simply turning on and off in a limited and predictable way, many genes---whose expression is controlled by their promoters---are expressed in bursts, with expression fluctuating randomly over time.

![Analyzing the nucleosome configuration of single gene molecules by electron microscopy.](pbio.1001622.g001){#pbio-1001622-g001}

What is the molecular basis of this transcriptional bursting? Previous work has suggested, but not directly demonstrated, that gene promoters can assume alternative structural configurations, raising the possibility that the promoter might randomly flip among these alternatives. Transcription requires that the polymerase machinery gain access to the promoter, and that access can be inhibited when DNA is tightly wrapped around histone proteins to form nucleosomes, the basic protein--DNA unit of structure within chromosomes. Researchersso as simply and with as few "working parts" as theirs---generally a sign that a model is pointing in the right direction.

**Brown CR, Mao C, Falkovskaia E, Jurica MS, Boeger H (2013) Linking Stochastic Fluctuations in Chromatin Structure and Gene Expression. doi:10.1371/journal.pbio.1001621**

[^1]: The author has declared that no competing interests exist.


(3) (1a) 3 × 5 Touch & Go Cleans (full) / 70% of 5RM / 90 s of rest.   (1b) 3 × 10 Strict Hand Standing Push up (as fast as possible)---2 min of rest.   12:00 AMRAP of: Row 250 m 25 6″ Target Burpees

*WOD, Workout of the day; AMRAP, as many round as possible*.

In workout of the day 2, subjects completed: (a) five sets of one repetition of clean and Jerk from the block at 80% of 1RM with 2--5 min rest intervals; (b) 3 sets of 5 touch and go cleans (full) with 70% of 5RM with 2--5 min rest; (c) 3 sets of 10 strict hand standing push-ups; After the third set of the aforementioned exercises, 5 min of rest was0.80, the sample effect size *f* was 0.65 for IL-6, 0.32 for IL-10, 0.49 for IL-10/IL-6 ratio, and 0.20 for OPG. The level of significance was *p* ≤ 0.05 and SPSS version 20.0 (Somers, NY, USA) software was used.

Results {#s3}
=======

The physical characteristics of the subjects are presented in Table [1](#T1){ref-type="table"}. Body fat and blood pressure were considered to be within normal range. Although both sessions of exercise elicited a significant increase in blood lactate and glucose concentrations, exercise training 1 induced a significantly higher increase than exercise training 2 (Figure [2](#F2){ref-type="fig"}).

![**Blood lactate (BLC; A) and glucose (BGC; B) concentration before and after workout of the day 1 and workout of the day 2**. Values are expressed as means ± *SD*. ^\*^*p* ≤ 0.05 to Pre; ^†^*p* ≤ 0.05


A collection of civil rights advocates filed suit against Ben Carson and the Department of Housing and Urban Development (HUD) on Tuesday over its delay of an Obama-era regulation designed to help cities and counties address segregation.

The Affirmatively Furthering Fair Housing (AFFH) rule was designed in 2015 to enforce a rule from the Fair Housing Act of 1968 that went largely ignored for 40 years. The AFFH rule requires municipalities to develop concrete plans to address segregation in their communities. Failure to do so would result in denial of money from the Community Development Block Grant program, which disperses $3 billion annually.

In January, Carson suddenly announced he was delaying the AFFH rule until 2020. But because the rule requires anti-segregation plans that are tied to the Assessment ofnow I've finished
installing it, let's close this,
and let's run it!
OK so the program has launched now let
me resize this window
a little bit to fit my screen here.
OK, so it seems that
I can do a new project here in the
project folder, a project name
I'm just not gonna bother moving stuff
so I'm just gonna call it
'first look video' and video mode, so the resolution and frame rate
based on the first file you
add that is fine by me and um
let's say if we wanted to edit video for
YouTube maybe, we could probably do an
HD 1080p at 29.97 or 25 frames per
second - let's try 25.
So if I specify this here apparently
it's going
to keep it at that resolution
no matter what video clips I'm adding so
let's click start.
OK so there is nothing here
very


basic
editing i'm not trying to do anything
advanced in here i'm just trying to
explore
the interface of this software so i have
the audio track
on a separate track here i have the
couple of video things so
let's see how we can add some titles if
we can
okay so the filters apparently if you
want to add some text you need to use
filters which sounds a little
unintuitive for me because
you know text is not really filters it's
more like a an asset or a title
so you need to go to filters here and
then you click this plus sign
and then you have a lot of stuff in here
you can add and you can have favorites
video and audio so if you go to video
then you see all of these things they
apparently are filters so
there's old film noise allenough for now i'm gonna
try to
export this video file now see how that
comes out
what happens with that so to export
let's try to go to file maybe you have
export video export an individual frame
apparently edl
this is like a file to dictate the
segments i believe
so export video let's see so we have all
these presets now
so the defaults create an h.264 aac
mp4 which is suitable for most users and
that is
true now you can use a hardware encoder
and then you have these presets and
let's try
h.264 high profile or maybe
does it give you presets like premiere
does with your youtube and all those
formats no they don't okay so
um i'm just gonna oh there it is youtube
okay
so i'm gonna i'm gonna select this
youtube
preset which is h.264 aac
audio and an mp4 container for youtube
so let's see


of outputs of the agriculture, manufacturing, and service sectors.

We have, therefore, placed much energy and enthusiasm into the development of this website to provide timely information on the Ministry, our agencies and programmes.an organization is much more than the field manager. We all share responsibility for the success and failure of the Boston Red Sox. We are not making a change in manager.

There has been no lack of effort from our players and we have had a number of them playing hurt. I watch every game and the effort our players put in night after night is very clear to see.

In regard to the notion that we have somehow not empowered Bobby, you should ask him directly about that. We have been nothing but supportive of him inside and outside of the clubhouse. Stories that imply otherwise are due to speculation that is not warranted at all by the facts.

And the notion that we are not present and not attending


Bladderball over a fence or out one of the gates of the Old Campus, whereupon a flying wedge of Yale Campus Police would seize possession of the Bladderball and hastily deflate it, terminating that year’s contest.

I recall that, one year, the undergraduate community bested the Campus Cops by successfully moving the Bladderball through New Haven streets for blocks and blocks, finally putting it over the fence into the yard of the residence of the University President on Hillhouse Avenue, as President Brewster cheered them on.

Since there was no actual set of rules or system of scoring, it was traditional for every team to compete in loudest, and earliest, and most preposterous claims of victory.

Student demand seems to have persuaded Richard Levin, the current Yale President, to do theand that the reevaluation was part of a broader reconsideration of the end-of-year events for plebes.

Alumni scoffed at the risk of someone’s getting hurt, especially given the school’s mission to prepare officers for combat.

Yale use to have a similar male-bonding event, the annual bladderball game, banned by President Bartlett Giamatti in a fit of politically correct namby-pambyness in 1982.


frontline title to launch alongside the Oculus Rift later this month.

What do you need to play it?

For the VR version, you’ll need a powerful PC and the Oculus Rift headset, amounting to a good $1500 worth of gaming hardware. The price predicament is one shared by all VR games of course, and on the bright side, once you’ve set yourself up with a VR-worthy rig then you can be damn sure that it will run non-VR games swimmingly for the next several years.

Adrift also comes in a non-VR flavour, which will be available to download on Steam for $20. This will also give you a free key to play the VR version via Oculus’ gaming platform, Oculus Home. While Three One Zero co-founder Adam Orth says that they’restill looking into the possibility of the Oculus Home version having its own price tag, that’s not been decided yet.

Orth is also keen to stress that Adrift isn’t an Oculus Rift exclusive, and will be coming to other VR platforms and consoles. However, he remains coy about whether it would be coming to the HTC Vive.

What do you do?

Explore a destroyed ISS-type space station in zero-gravity conditions, trying to work out what the hell went wrong and - most importantly - making sure you don’t run out of air by taking hits from the oxygen canisters floating around the place.

The first few seconds of Adrift are breathtaking, as you come to in a large glassy room, floating amidst plenty of exceptionally pretty and detailed detritus, with the curvature