feel how young I was. I mean, don't get me wrong, I'm still young (25 in February, please make donations to the Kickstarter fund I am considering setting up to help fund my life), but just reading that post from two years ago, I realize how much I've grown since then...

I am 22-years young, direction-less, and loving it! I use emoticons like it's my job, and love to write! I have been plagued with the need to travel and explore the world we live in, and cannot rest until I have done so.

I still remember that being my catchphrase back then -- young, direction-less, and loving it! And I really was! I was so young, I had noooo direction, nor could I have cared less! I loved thewilder one it will be! I hope you'll continue to join me, and hey, come on out and make it your reality too, whenever you want!

Please remember how capable and powerful you are. There is something you are here to do; and if you get a sense for it, run with it, sprint towards it, and give it all you've got, you will achieve and receive endlessly. As long as you follow your heart, let God be your guide, and pursue your passions, there can be nothing but goodness to come from it. You're magic!

Enjoy life!! There is no limit to what can be done while we're here!

Sending you sweet, sweet blessings, and abundant amounts of love & light on this beautiful day!

Wednesday, December 11, 2013

This past weekend


up another country, and this time I decided to make my bridge in.....

Copenhagen, Denmark!!

Copenhagen had been on my Hit-List since I studied abroad in Bilbao over three years ago. I think the draw initially came from hearing stories from people who had gone, as well as from the Dane that was in our study abroad group first semester. Stories I was told, and videos I was shown, during my days as The Party Girl, about how crazy and wild the parties there are. Especially since it is one of the world capitals, and the most progressive, innovative, and happy cities, it just made sense to hit it up at some point.

Luckily for me, one of my friends from home has been studying there this semester, and when Iproper night's sleep leading into the trip. I had roughly two, three-hour naps, one in the airport on a marble window sill, the other on the plane. So as you can imagine, I was going into the trip with a mind that was all floppy, all over the place.

It would have been wise to use the day when I arrived to go and see stuff, considering that while it was cold, it was actually sunny, and I had arrived early enough that we could have had a full day of exploring. But I really wanted to do little other than relax, so we ended up just hanging out at his place, catching up, and chilling with some of his study abroad friends.

That night we went to a dinner


the markets gaping at everything around us, we walked through the city center and by some important looking buildings. Since it is Christmas time, the lights were all up, and all on, giving the city a dreamy feel, and a sense that there was nothing inside the Copenhagen borders other than happiness and joy, and nothing outside them at all worth experiencing. The city center was a thing of wonder! It's your typical city center, designed for the people to come and spend money, but with the feeling of that place, it was like walking through a fairy tale land. I'm not sure if it's because the countries up north are so clean and organized and just have that general North Pole feeling, but I really didn't believet naught is zero and it starts from rest and we'll take this position to be zero that's all usual assumptions and then the ending point has a position of 100 meters exactly because this is a 100-meter dash and we are told that the total time is 9.69 seconds so he'll cross this position at that time. And then we are told that at this time and we don't know precisely where to put this on the picture because we don't know what this position is but we do know the time is 3 seconds here when his acceleration suddenly stops and he just maintains some maximum speed that we call v which we have to figure out for this time period here. Okay. So we are


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My problem, I would like to registera less severe course of disease (Singh, S. R. et al., 2007. Immunogenicity and efficacy of recombinant RSV-F vaccine in a mouse model. Vaccine, 25(33), S.6211-6223., Zhan, X. et al., 2007. Respiratory syncytial virus (RSV) F protein expressed by recombinant Sendai virus elicits B-cell and T-cell responses in cotton rats and confers protection against RSV subtypes A and B. Vaccine, 25(52), 5.8782-8793., Vaughan, K., et al., 2005. DNA immunization against respiratory syncytial virus (RSV) in infant rhesus monkeys. Vaccine, 23(22), S.2928-2942).
Moreover, it could be shown that a balanced regulatory and effector T cell function is required for viral clearance and reduction of severity of illness (Liu, J. et al., 2010. Epitope-specific regulatory CD4 T cells reduce virus-induced illness while preserving CD8 T-cell effector function at the site of


of an antigen to the immune system or induction of an unspecific innate immune response. Furthermore, an adjuvant may preferably e.g. modulate the antigen-specific immune response by e.g. shifting the dominating Th2-based antigen specific response to a more Th1-based antigen specific response or vice versa. Accordingly, an adjuvant may favourably modulate cytokine expression/secretion, antigen presentation, type of immune response etc.
Antigen: According to the present invention, the term “antigen” refers typically to a substance which may be recognized by the immune system and may be capable of triggering an antigen-specific immune response, e.g. by formation of antibodies or antigen-specific T-cells as part of an adaptive immune response. An antigen may be a protein or peptide. In this context, the first step of an adaptive immune response is the activationthe like. However, indirect antigenic functionality typically means in this context that the “antigenic function” is, e.g., a nucleic acid sequence that is taken up by the target organism and translated within the organism into a peptide or protein. This peptide or protein then functions as an immunogen and induces an adaptive immune response. Thus, in one variant, an “antigenic function” is understood to be a preform or precursor of an immunogen. Also, an “antigenic function” can be understood to be an immunogen itself. In the context of the present invention, an antigenic function may in particular be a Fusion (F) protein of the virus family Paramyxoviridae and (e.g. artificial) functional variants or fragments thereof as well as (preferably immunogenic) fragments of said Fusion (F) protein and respective


variants; as well as corresponding nucleic acids encoding any of these, i.e. Fusion (F) proteins of the virus family Paramyxoviridae, variants thereof as well as fragments of said Fusion (F) protein and respective variants. In the context of the present invention, an antigenic function may also in particular be a Hemagglutinin (HA) protein of the virus family Orthomyxoviridae and (e.g. artificial) variants thereof as well as (preferably immunogenic) fragments of said Hemagglutinin (HA) protein and respective variants; as well as corresponding nucleic acids encoding any of these, i.e. Hemagglutinin (HA) proteins of the virus family Orthomyxoviridae, variants thereof as well as fragments of said Hemagglutinin (HA) protein and respective variants. Fusion (F) proteins of the virus family Paramyxoviridae and their amino acid sequence and (e.g. artificial) variants thereoflevel or on amino acid level.
Carrier/polymeric carrier: A carrier in the context of the invention may typically be a compound that facilitates transport and/or complexation of another compound. Said carrier may form a complex with said other compound. A polymeric carrier is a carrier that is formed of a polymer.
Cationic component: The term “cationic component” typically refers to a charged molecule, which is positively charged (cation) at a pH value of typically about 1 to 9, preferably of a pH value of or below 9 (e.g. 5 to 9), of or below 8 (e.g. 5 to 8), of or below 7 (e.g. 5 to 7), most preferably at physiological pH values, e.g. about 7.3 to 7.4. Accordingly, a cationic peptide, protein or polymer according to the present invention


the release of various cytokines in response to an antigen. In a more general way, cellular immunity is not related to antibodies but to the activation of cells of the immune system. A cellular immune response is characterized e.g. by activating antigen-specific cytotoxic T-lymphocytes that are able to induce apoptosis in body cells displaying epitopes of an antigen on their surface, such as virus-infected cells, cells with intracellular bacteria, and cancer cells displaying tumor antigens; activating macrophages and natural killer cells, enabling them to destroy pathogens; and stimulating cells to secrete a variety of cytokines that influence the function of other cells involved in adaptive immune responses and innate immune responses.
Combination vaccine: A combination vaccine is typically a vaccine that may provide two or more immunogens and/or antigenicpreferably at least 8 amino acids, even more preferably at least 9 amino acids; even more preferably at least 10 amino acids; even more preferably at least 11 amino acids; even more preferably at least 12 amino acids; even more preferably at least 13 amino acids; even more preferably at least 14 amino acids; even more preferably at least 15 amino acids; even more preferably at least 16 amino acids; even more preferably at least 17 amino acids; even more preferably at least 18 amino acids; even more preferably at least 19 amino acids; even more preferably at least 20 amino acids; even more preferably at least 25 amino acids; even more preferably at least 30 amino acids; even more preferably at least 35 amino acids; even more


more amino acids, e.g. 13, 14, 15, 16, 17, 18, 19, 20 or even more amino acids, wherein these fragments may be selected from any part of the amino acid sequence. These fragments are typically recognized by T cells in form of a complex consisting of the peptide fragment and an MHC molecule.
B cell epitopes are typically fragments located on the outer surface of (native) protein or peptide antigens as defined herein, preferably having 5 to 15 amino acids, more preferably having 5 to 12 amino acids, even more preferably having 6 to 9 amino acids, which may be recognized by antibodies, i.e. in their native form.
Such epitopes of proteins or peptides may furthermore be selected from any of the herein mentioned variants of such proteins or peptides.family Paramyxoviridae, and the RNA encoding the Hemagglutinin (HA) protein or a fragment, variant or derivative thereof of the virus family Orthomyxoviridae are comprised in the same composition of the combination vaccine. One single composition enables the locally and timely simultaneous application of different antigens, which may be considered to be particularly advantageous in this specific application, because it improves the T cell response directed against the F protein. Furthermore, it reduces the number of injections required to prevent the diseases and minimizes the costs of stocking separate vaccines.
Quasi-simultaneous administration may, alternatively, be also achieved by subsequent administration (within e.g. up to 10 minutes, more preferably within two minutes) of a combination vaccine which is composed of e.g. two separate compositions, wherein the first composition contains RNA encoding


(HA) protein that can individually act as immunogens. These epitopes of each of these proteins are preferably arranged in a non-native way, which means that the epitope sequences are isolated from the native sequences and are linked by non-native linker sequences (e.g linker sequences having more than 50% glycine and proline residues). Generally, however, inventive monocistronic RNAs encoding such fusion proteins may be provided with or without linker sequences. Such linker sequences typically comprise 5 to 25 amino acids, preferably selected from proline and glycine. Preferably, the linker sequence is immunologically neutral. e.g. non-immunogenic and non-immunostimulatory.
It is preferred that the at least one Fusion (F) protein is derived from viruses selected from: Avulavirus, Ferlavirus, Henipavirus, Morbillivirus, Respirovirus, Rubulavirus, TPMV-like viruses, Pneumovirus, Metapneumovirus, Atlantic salmon paramyxovirus, Beilong virus, Jusually a so-called cap structure, which is a modified guanosine nucleotide also called 5 ‘Cap structure, and at the 3’ end is typically a sequence of up to 200 adenosine nucleotides (the so-called poly-A tail). By a further embodiment the at least one RNA comprises at least one of the following structural elements: a histone-stem-loop structure, preferably a histone-stem-loop in its 3′ untranslated region, a 5′Cap structure, a poly(C) sequence, a poly-A tail and/or a polyadenylation signal, preferably as defined herein.
By a further embodiment, the at least one RNA preferably comprises at least two of the following structural elements: a 5′ and/or 3′-stabilizing sequence; a histone-stem-loop structure, preferably a histone-stem-loop in its 3′ untranslated region; a 5′-Cap structure; a poly(C) sequence; a poly-A tail; or a polyadenylation signal,


of who we are as adults. Will Agnes and Elin’s relationship last? Who knows, but the bigger message of this movie is over coming what other people think of you and becoming your own person.– they are actually the ones who are showing them what is wrong and what should be improved. But black hat hackers congregate on the dark web and discuss these vulnerabilities and I won’t be surprised that Mr. Sharma is a subject of plots in the dark web discussion forums by really talented but criminal hackers.

A 2017 compendium drafted by UIDAI on Aadhaar observed that Aadhaar can now be de-linked from any account. Is it necessary?

I think if it is enabled – people should delink. But the problem is that most entities like banks, mobile phone companies, insurance companies and anyone else who took your Aadhaar data via e-KYC have already seeded their local databases with Aadhaar. Much of that might not even be under the jurisdiction of


each network type, we generate 300 data sets with a sample size of 100, 200, 500 and 1000. Further, for each of these data sets, a bootstrap ensemble of size *b* = 100 was generated by sampling with replacement.

In addition, we generate simulated gene expression data sets for a network consisting of 8 network modules, which are organized in a hierarchical manner; see Figure [4](#F4){ref-type="fig"} for a visualization. Each network module is generated using a *Modular Topology Model* (MTM) network model, each with a size of 25 genes. A MTM network has properties such as a scale-free degree distribution, high clustering coefficients and short path lengths as observed in real biological networks \[[@B32],[@B33]\]. We construct 5 different networks by weakly connecting the 8 individual modules with a differentmicroarray data sets
-----------------------------------------------------

The collection of the microarray gene expression data used in this study are from \[[@B21]\], which are accessible from the NCBI *Gene Expression Omnibus* (GEO) \[[@B34]\] (accession GSE2350). We denote the GSE2350 dataset that includes transformed and untransformed B-cell lymphoma samples as the *Basso GSE2350 dataset*. For our analysis we consider only samples for which raw gene expression data in form of CEL files are available. From the total of 387 samples of the GSE2350 dataset, 344 samples were available with raw CEL files. In the following, we call this data set *D*. The data set includes two Affymetrix chip platforms, *hgu95a* and *hgu95a_v2*. We used the mixture CDF environment hgu95av12mixcdf_1.0.tar.gz available from http://bmbolstad.com/misc/mixtureCDF/MixtureCDF.html to include only probe sets that have the same probe set annotation.

For


networks, *G*~*a*~ and *G*~*b*~, we estimate the betweenness centrality values for all genes from a Gene Ontology (GO) term. Then, for each GO term, we perform Spearman's rank correlation test \[[@B43]\] for the ranks of the betweenness centrality values. We adjust p-values using a FDR \[[@B45]\] correction for a given significance level of *α* = 0*.*05. For the analysis we use the Gene Ontology (GO) annotation from the Bioconductor *org.Hs.eg.db* package.

Hierarchical network organization
---------------------------------

In order to analyze the hierarchical organization of the B-cell C3NET gene regulatory network, we perform a three-step procedure based on bootstrap samples of the data. In the first step, we infer a network *G* by using all 344 samples of the microarray data set *D*. For this network, we identify its network components, which representclustering error from the lowest to the highest split *s*~*i*~ in the dendrogram. The maximal number of clustering errors is the total number of splits defined in the dendrogram. An example for the calculation of the error score *d*~1~ is shown in Figure [6](#F6){ref-type="fig"}. In order to obtain a null model of the dendrogram clustering error that corresponds to the random clustering of network modules, we generate reference networks with permuted module labels. That means, we assume a network with a modular structure as shown in Figure [4](#F4){ref-type="fig"}, but permute the labels of the corresponding modules within this network.In Figure [8](#F8){ref-type="fig"} A we show the empirical cumulative distribution function (ecdf) of the dendrogram clustering error *d*~1~ for a variety of networks with different numbers of module interconnecting edges.


*d*~2~ decrease with an increasing number of interconnecting edges between the network modules. This means adding edges between the network modules helps in reducing the inference error. For the networks with no interconnections between the network modules (black) *d*~2~ is largest, as expected. These results correspond to the absence of a hierarchy between the network modules. Overall, the results for *d*~2~ are similar to *d*~1~ demonstrating that regardless of the chosen error measure a relatively low number of interconnecting edges is sufficient to enable the recovery of at least parts of the present hierarchy in the network.

Analyzing network components of the B-cell C3NET gene regulatory network
------------------------------------------------------------------------

We infer a C3NET \[[@B18]\] gene regulatory network from 344 samples of the B-cell lymphoma microarray gene expression data set from \[[@B21]\]. Thethe subgroupings between the functional and structural hierarchical clustering differ to some extend, overall, the network components of the two DLBCL gene regulatory networks show a similar clustering into two major branches of peripheral and intracellular regions. However, the bipartiton of the structural network components (second column in Figure [10](#F10){ref-type="fig"}) is less pronounced as observed for the B-cell C3NET gene regulatory network for the *Basso GSE2350 dataset*.

Identification of novel key signaling pathways in B-cell lymphoma
-----------------------------------------------------------------

Hub genes of the B-cell C3NET gene regulatory network are genes with the largest node degree among all genes in the network. Intuitively, such genes are the most interesting targets to study as they are more likely to be associated with multiple pathways, e.g., signaling pathways and thus form putative key regulators for a


the DLBCL-C3NET gene regulatory network obtained from the *Lenz GSE11318 dataset*, we identify 95 (21%) gene sets, and for the comparison of the B-cell C3NET gene regulatory network with the DLBCL-C3NET gene regulatory networks obtained from the *Salaverria GSE22470 dataset*, we find 72 (16.5%) gene sets with a statistically significant correlation. In total, 58 terms are simultaneously significant in both network comparisons. These terms involve the *basal part of cell*, *cell periphery*, *endosome* and 17 gene sets sharing the parental term *GO:0032991 macromolecular complex*, e.g., *histone mehyltransferase complex*, *anaphase-promoting complex*, *ribosome* and *cation chanel complex*. In Table [5](#T5){ref-type="table"}, we show the 30 Gene Ontology *cellular component* gene sets with the highest structural similarity between the B-cell C3NET gene regulatory network and the two DLBCL-C3NET gene regulatory networks. Eachto a fundamental organization scheme of cellular networks. A potential hypothesis derived from these results is that the hierarchy among the network components may reflect aspects of the information flow between these components \[[@B62],[@B63]\].

There are several advantages resulting form our approach, we would like to highlight. First, our investigation of the hierarchical organization of the B-cell C3NET gene regulatory network is at the abstraction level of *network components* or modules, but not *genes*. As such it resembles a systems approach \[[@B64]-[@B66]\]. This leads to a tremendous reduction in the complexity of the problem, and specifically in the interpretation of the obtained dendrograms shown in Figure [10](#F10){ref-type="fig"}. Second, on a technical note the size of the bootstrap ensemble was chosen large enough so that a further increase in its


area of \~24,400 Å^2^, and with a largely positive electrostatic distribution across the catalytic domain and largely apolar distribution across the PG and scaffolding domains. An extended electropositive active site demarcated by bound meropenem antibiotic lies across the catalytic domain with appropriate clefts for both donor and acceptor substrates adjacent and continuous (Fig. [1](#Fig1){ref-type="fig"}). The observed overall architecture of YcbB (Fig. [1](#Fig1){ref-type="fig"}) has several distinct features from all currently deposited L,D-transpeptidase structures, as suggested from the unique insertions and substitutions in sequence alignments with previously characterized Gram positive and mycobacterial species (7.1--14.3% sequence identity; Supplementary Fig. [2](#MOESM1){ref-type="media"}). The well ordered central catalytic domain, comprised of residues 375--576, forms a canonical L,D-transpeptidase fold harbouring an extended active site cleft with the expected conserved active site motif^[@CR10]^ (HX~15--18~\[S/T\]XGC*h*\[R/N\], where X represents any residue, andlabelled in red. **b** Overlay of YcbB peptidoglycan binding domain and high scoring peptidoglycan binding domains from a structural homology search on the Dali server^[@CR14]^. YcbB in red, gp144, a *Pseudomonas aeruginosa* phage phiKZ endolysin in salmon (PDB ID 3BKH^[@CR15]^), a *Clostridium acetobutylicum* zinc-dependent amidase in white (PDB ID 4XXT), a *Clostridium difficile* PBP in pale blue (PDB ID 5TV7), and gp15, a *Burkholderia* AP3 phage endolysin in dark blue (PDB ID 5NM7^[@CR11]^). Peptidoglycan binding domain structures are coloured from red to blue based on decreasing structural homology. **c** The sequence of *E. coli* YcbB (YcbB_Ec) aligned with peptidoglycan binding domains from phiKZ gp144 (gp144_phiKZ), *C. acetobutylicum* zinc-dependent amidase (Zn_amidase_Ca), *C. difficile* PBP (PBP_Cd), and AP3 gp15 (gp15_AP3). Secondary structure of *E. coli* YcbB is displayed atop the


coli* YcbB catalytic domain. **a** A ribbon representation of the YcbB catalytic domain, residues 375--576, coloured in rainbow from red (N-terminus) to blue (C-terminus). Meropenem is represented in black and coloured by heteroatom. The unique capping sub-domain is highlighted in a bold silhouette. **b** The catalytic domain architecture of *Enterococcus faecium* Ldt~Fm~ (PDB ID 3ZGP^[@CR17]^) and *Bacillus subtilis* YkuD (PDB ID 1Y7M^[@CR13]^) highlighting the differences seen among L,D-transpeptidase catalytic domains in the capping loop region. **c** Cut surface representation of the YcbB catalytic domain, showing the capped cleft formed between the donor and acceptor sites by the capping loop sub-domain. The catalytic Cys528 and His509 are coloured in yellow and blue respectively. Meropenem (blue) is overlaid in its position in the donor site, acylating the catalytic cysteine. **d**other species, it is not completely conserved at this position in the L,D-transpeptidase motif. The specific nature of these protonation events can perhaps be unravelled in future Michaelis complex structures of L,D-transpeptidases.

Inhibition of the L,D-transpeptidase mediated resistance pathway {#Sec8}
----------------------------------------------------------------

In order to further probe the inhibition of the YcbB-mediated beta-lactam resistance pathway, meropenem acylation of both the L,D-transpeptidase and D,D-carboxypeptidase activities required was investigated (Fig. [4](#Fig4){ref-type="fig"}). The YcbB-meropenem complex map had ordered density defining the active site residues including the thiol ester covalent link of Cys528 and acylated meropenem (Fig. [4b](#Fig4){ref-type="fig"}). A 2.2 Å resolution structure of PBP5 acylated with meropenem was also solved with very well ordered density for the covalently bound meropenem (Fig. [4c](#Fig4){ref-type="fig"}). Relative to previously published PBP5 structures, we were able to trace additional residues at the C-terminus of PBP5


beta-lactam resistance apart from carbapenems. Unsurprisingly, replacements of both the catalytic cysteine and histidine resulted in a complete loss of the beta-lactam resistance (β-lac^R^) phenotype seen in the assay with wild type YcbB.Fig. 5In vivo assay of YcbB mediated beta-lactam resistance. **a** Various replacements and deletions of the catalytic residues (in red), capping loop region (in green) and PG binding domain (in blue), mapped onto the catalytic and PG domains of YcbB. Replacements retaining YcbB mediated beta-lactam (ampicillin and ceftriaxone) resistance are denoted as β-lac^R^, while replacements and deletions resulting in beta-lactam (ampicillin and ceftriaxone) susceptibility are denoted as β-lac^S^. **b** Replacements and deletions of pTK2(*ycbB*) (i--viii) are listed with their resulting phenotype

Of greater interest was the contribution of the capping loop to antibiotic resistance. To this end,occur involving rearrangement of the substrate capping loop/sub-domain and resulting in the capped substrate cleft (seen in the acyl-enzyme complex structure) to re-orient into an open cleft. This proposed mechanism for this resistance pathway is consistent with the growing understanding in the field that PG biosynthesis depends on a number of dynamic and variable multi-protein complexes^[@CR26]^ that are regulated spatiotemporally and, as in the case of YcbB mediated beta-lactam resistance, by external stimuli. As we enter into an era of increasing antibiotic resistance, unravelling the intricacies of these non-canonical PG biosynthetic complexes will be of increasing importance as bacteria deploy these flexible pathways to evade antibiotic assault.Fig. 6Schematic representation of YcbB mediated beta-lactam resistance. **a**--**e** Assembly and function of the YcbB mediated beta-lactam resistance pathway. **a** PBP1b function


catalytic domain during L,D-transpeptidation. **f** Tetrapeptide of the donor PG strand enters the donor site of the catalytic domain. **g** Donor tetrapeptide is acylated at its *meso*-DAP residue by the catalytic cysteine, resulting in the loss of the terminal D-alanine. **h** Adjacent acceptor PG peptide enters the acceptor site of the catalytic domain. **i** The covalent acyl-enzyme is deacylated via nucleophilic attack of the side chain *meso*-DAP on the adjacent acceptor PG strand, forming the L,D-crosslink. **j** Rearrangement of the capping loop/sub-domain from a capped conformation to an open cleft will occur, resulting in the release of the crosslinked substrate

Methods {#Sec12}
=======

Cloning and protein expression {#Sec13}
------------------------------

*Escherichia coli* YcbB without its signal peptide (residue 31 onward) was cloned into the expression vector pET41b-GST with a thrombin cleavable, C-terminal 8x His-tag.lysed by processing twice with a homogenizer (15 kPa; Avestin). Cellular debris was pelleted by centrifugation at 125,000 × *g* for 1 h. The resultant supernatant was loaded onto a 1/5 mL Ni^2+^-saturated HisTrap HP Sepharose cartridge (GE Lifesciences), washed with 75 mM imidazole in Buffer A (20 mM Hepes, pH 8.0, 300 mM NaCl), and the protein was eluted with 300 mM imidazole in Buffer A. 1 U of HRV 3 C protease or thrombin was added per mg of protein to remove the N-terminal His-tag overnight at 4 °C. Samples were purified further by size exclusion chromatography (SEC) with a Superdex 200 column (GE Lifesciences) equilibrated in Buffer B (20 mM Hepes, pH 8.0, 150 mM NaCl). Fractions containing pure PBP5 or YcbB were pooled and concentrated to 10 to 40 mg/mL. Protein was frozen rapidly in liquid nitrogen and


Superdex 200 column (GE Lifesciences) equilibrated in Buffer B with 0.2% DM. Fractions containing pure PBP1b were pooled and concentrated to 10 to 40 mg/mL. Protein was frozen rapidly in liquid nitrogen and stored at −80 °C until required. SMA-solubilised PBP1b was prepared as above, with the extraction occurring in 2% activated SMA and subsequent buffers having no detergent present.

Microscale thermophoresis {#Sec15}
-------------------------

MST assays were conducted using a Monolith NT.115Pico (NanoTemper). YcbB, PBP5 and PBP1b were fluorescently labelled using Alexa Fluor 647 NHS Ester (Thermo Fisher Scientific). To evaluate the binding of PBP1b to YcbB, increasing concentrations of unlabelled YcbB (1.5 nM--3.1 µM) were used to titrate fluorescently labelled SMA-solubilised PBP1b at a constant concentration (100 nM). To evaluate the binding of PBP5 to YcbB, increasing concentrations of unlabelled PBP5 (120 pM--98 µM) were used tolong it took to get the game out. Did you manage to meet what deadlines were set?

Blackman: Yeah, for the most part. The great thing about working at 2K is that the game comes first. Figuring out what the core of the game is took us some time, and we wanted to make sure everyone was on board with that. Not just from a concept standpoint, too. Early on we knew who, when, and where the game was going to be, but talking about the scope and the core gameplay mechanics, all of that took a bit longer in preproduction.

Once we had all that, we were off to the races. Like a lot of teams, we did user testing and focus testing. We got a lot of feedback


The sitdowns, too, were an idea we always had, but when it came to the priorities of development and other things we had to work on, getting some of the foundational things in, the sitdowns ended up materializing really late in development. We had to do a lot of iteration on those. I’m happy that they turned out as well as they did given how late they came in.

Some of the conversations between Lincoln and the contact characters — conversations with Nicki and whatnot – those came in really late, just to close the loop on a few narrative moments. That I wish we’d had some more time with.no scrollbar.
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(827 F.2d at 172), and held that the Hauptli lease was for an indefinite term, so that an investment tax credit was denied.2

6
Because of our conclusion that these leases were not for a fixed term, it is unnecessary to consider what party bore the economic risk with respect to the property.   Neither is it necessary to consider whether the cylinders' useful life was only nine years (as suggested alternatively below by the Commissioner) so that if the leases were indeed for five years, they would still not satisfy the statutory requirement that the leases be less than 50% of the useful life of the cylinders.

7
The decision of the Tax Court is affirmed.

1
 This provision was repealed in 1984 for tax years after December 31, 1983 (Taxover an extended period of time before being assembled together to an electrode assembly. In result of the heat treatment, the electrodes will be partially or completely re-crystallized and the out-gas-able components will be removed in order to provide for a better adhesion between the electrode and the seal wall material and to reduce crack failure of HID lamps. This method and the resulting electrode is much too expensive for mass production and the time-consuming heat treating makes the manufacturing process difficult and ineffective. In addition to this, it is not guaranteed that the crack pattern is constant with a controlled crack form.
Thus, there is a particular need for a HID lamp with an electrode seal structure, which is capable of resisting high thermal and mechanical stress due


anterior descending coronary artery (LAD) occlusion followed by reperfusion results in dramatic increases in ischemic levels of interstitial fluid adenosine and postischemic myocardial function. Using a similar model in dogs, we have shown that exogenous intracoronary adenosine (50 micrograms/kg per min) augmented postischemic recovery of function, as assessed by significant enhancement (p < 0.01) of systolic wall thickness (7.0 +/- 3.0 pretreatment vs -5.7 +/- 1.7 controls). These data support the role for an adenosine A1 receptor mediated mechanism for protection against myocardial stunning.direction expeditiously. Captain Sully considered three options for landing in less than a minute. He then decided, “We’re going into the Hudson.” Once he made that decision all other options were cut off. Decision means to cut or take away. Once the other two options for landing the plane were taken away he and the crew could focus completely on making a successful water landing.

Lesson #3: Do not multi-task. Concentration and focus is the discipline of champions and heros. When asked if he prayed, the captain said he was concentrating on landing his plane and he “thought of nothing else.” Focus is seeing all the options clearly, but concentration is executing those options intently. You will not lead your company through this economic crisis if you are distracted


instruction to the passengers because he knew the team was on the same page. How many injuries were averted and deaths prevented by this amazing team effort? We will never know because everyone lived. Are you giving timely and clear directives to your team? Are you on the same page?

Lesson #6: Preparation is the mother of competence. I am moved by Captain Sully’s quite confidence when he said, “I was sure I could do it.” There were no second attempts, no “do-overs”. Just one shot. Pull it off and everyone lives. Fail and everyone dies. He believed his life was preparation for that single event. Captain Sully was prepared for the task and he executed on what he knew without thinking about it. That is all about beingA long-term clinicopathologic survey of patients with Jessner's lymphocytic infiltration of the skin.
Skin biopsies from 6 patients with Jessner's lymphocytic infiltration (JLI) were studied using monoclonal antibodies in peroxidase staining, on some occasions combined with [3H]thymidine incorporation visualized by autoradiography. Ninety-one +/- two percent of all inflammatory mononuclear cells in situ were T11-positive T lymphocytes, whereas B lymphocytes were few. Forty-nine +/- nine percent of cells were Ia-positive, suggesting involvement of T cells in the local pathogenetic mechanisms, but interleukin-2 receptor-carrying cells as well as [3H]thymidine-incorporating cells accounted for less than 2% of all inflammatory cells, suggesting that T blasts account for only a small minority. Similarly, PCA-1 plasma cells were few in situ, there was no immunoglobulin or complement deposition at the dermal-epidermal junction and serum antinuclear


equivalent to those of HTLV-I-uninfected clones. On the other hand, during the IL-2-independent stage, expression of CD3-TCR complex on the cell surface was markedly decreased, and no significant elevation of intracellular free Ca2+ concentration was detected in response to anti-CD3 mAb. These data indicated that the loss of cytotoxic activity of HSV-specific T cell clones observed early after HTLV-I infection was not the result of impaired antigen recognition via the CD3-TCR complex, but might be due to dysfunction in the effector phase. On the other hand, the dysfunction of helper activity found late after HTLV-I infection might have mainly occurred in the recognition phase due to the decreased expression of CD3-TCR complex. The present data appear to suggest certain aspects of the pathogenesis of the immunodeficiency occurring incomprised of many separate pieces of parts therefore naturally result in complicated in design, expensive to manufacture, inefficient in operation and inconvenience in use. More particulary, these prior arts places more emphasis on the storage of ashes and butts and adopted the system to supply air into the holder or discard smoke from the holder through numerous small perforations or a tube. In this manner smooth combustion of cigarette may be jeoparadized due to the insufficient amount of oxygen or excess amount of smoke in the holder. Also, cigarette tars and nichotines will adhere to the walls of perforations or tube and requires special cleaning of the holder.


3 / 7
). In Python, we express the previous sentence as

>>> 10 % 7 3 >>> 3 % 2 1 >>>

Once you understand modulus, the divmod() function, which we'll discuss
in a later chapter, should come easily to you.

Does both of the division operators at once and returns a tuple; the second
item in the tuple contains the remainder. divmod(x,y) is equivalent to
x/y,x%y

This:

>>> divmod (10,3)

(3, 1)

Is the same as this:

>>> 10/3,10%3

(3, 1)

This:

>>> divmod (5,2)

(2, 1)

Is the same as this:

>>> 5/2, 5%2

(2, 1)

abs

Finds the absolute value of a number

>>> abs(100)

100

>>> abs(-100)

100

-,

Sign

>>> 1, -1, +1, +-1

(1, -1, 1, -1)

Numeric Conversion Operators

Many times we need to convert from one numeric type to another. The three
operators that perform this conversion are Int(x), Long(x), and Float(x), where
x is any numeric value. To illustrate, in thenasty trio we’d all have avoided like a plague of plagues, but representing just the right moment, says Luke, for God to act, and to do so through an unforgettable fellow named John, who emerged from the desert on strategic assignment.

So far, you might wonder what any of Luke’s agenda has to do with Christmas. But wait: isn’t Luke the Gospel writer who gives us most of what we know about the birth and infancy of Jesus? He must know what he’s up to… but what is it?

If we cheated and jumped ahead to next Sunday’s Gospel, would it help? There we’ll find two women and the babies they’re carrying in utero, Mary chambering Jesus in her womb and her relative Elizabeth sheltering John in hers—the same John


nivel 'info' para que la salida sea recogida por ambos transportes (file y console)
      logger.info(message);
    },
  };

module.exports = logger;

My nginx server configuration
server {
    listen 80;
    listen [::]:80;

    #your subdomain
    server_name api.mydomain.com;

    location /v1/ {
        rewrite ^/v1/?(.*) /$1 break;
        proxy_buffering off;
        #your aplication port
        proxy_pass http://localhost:9000;
    }

    location /start/ {
        rewrite ^/start/?(.*) /$1 break;
        proxy_buffering off;
 Q:

What are the advantages of using LISP and Haskell? Will they make me a better programmer?

I know Lisp and Haskell are logic and functional programming languages respectively, but what exactly does this mean? How do they differ from other languages? I've heard that learning these will make you a better programmer and improve your logic. Is this true, and if I go learning Lisp or Haskell to a competent level will my programming improve and will I be better at handling any problem in any language? I just wanted to know to see if they are worth the effort of learning. Also are these languages useful in areas such as GUI and graphics or are they just useful for console applications?

A:

It is very much like learning math will


take the attention off of where it should be, which are the playoff games. Kobe is an attention whore and disgusts me. Ok, my rant is over....

_________________Regards,

Tim

May 31st, 2007, 9:20 am

theAlphaMale

Site Admin

Joined: August 6th, 2004, 1:25 amPosts: 4920Location: Hills of Auburn

Maybe we could trade Dale Davis and Terry Mills for Kobe

_________________Regards, Alpha|Lionbacker.com \(^o^)/"I date this girl for two years and then the nagging starts: I wanna know your name..." - Mike Binder

May 31st, 2007, 9:26 am

conversion02

RIP Killer

Joined: January 26th, 2005, 9:34 pmPosts: 10493Location: Sycamore, IL

Anybody that puts up the kind of numbers Kobe does can dictate whether or not they want out of a place. When you're the only player on your team that is a threat, you get sick of it as an individual and as aMatt Kenner has been making a splash with his autocross C10 on courses all over the South. In fact, Matt has racked up a string of wins and podium finishes with his truck. Recent bragging rights include the fastest registered truck at the 2019 C10 Nationals, Fastest truck at the 2019 NSRA Nationals StreetKhana autocross in Louisville and Goodugys shootout winner in the truck class in Columbus.

More and more autocross trucks have been showing up at events around the country. And more often than not they are a Chevy C10. AutoXandTrack previously featured an autocross F100 so we were remiss on getting a C10 featured on the site.

Humble Beginnings

Matt didn’t start out to build an autocross truck. Instead he bought his 1968 C10 almost 20 years ago to


love jihad idea has ruptured what remains of Uttar Pradesh’s social fabric Shandar Ghufran

“We left our village the moment we heard news that a riot had broken out. That was the mistake we made,” recounted Mohammed Aslam, as he sat hunched on a string cot beside a tent. “We should have waited for someone to get killed first.”

The government, Aslam said, does not consider his village to be riot-affected and hence he is ineligible for the riot compensation of 500,000 rupees (about £5,000) per family. So far, 768 families have been granted compensation, and the supreme court has ordered the state government to compensate another 203 people. Yet the administration is in a bind: it needs a framework to distribute the compensation, without which it could be accusedare against deception and forcible conversion,” he said. He referred to Muslim Bollywood superstars with Hindu wives. “In most cases, the women are brainwashed and converted. Like Indira Gandhi.”

“Indira Gandhi?”

“Yes, she was married to Feroze Gandhi – but he was actually Feroze Khan, a Muslim. She was the first victim of love jihad.”

“But Feroze Gandhi was Parsi.”

“That’s what you think, Aman-ji, that’s just what you think. Everyone knows Feroze Gandhi Khan was a Muslim. It’s all over the internet.” •

This is an edited extract of Love Jihad from the current issue of Granta, India – Another Way of Seeing.

Follow the Long Read on Twitter: @gdnlongread


reduction from our (NP) problem to a known NP-complete problem due to the definition of NP-completeness*.

We give a proof of the following fact which is pointed out by Song, Gallos, Havlin and Makse[@b9].

**Proposition 2**. *For any fixed size l, the l-box-covering problem is NP-complete*.

*Proof*. If *l* = 2, *l*-box-covering problem is exactly the clique covering problem, which is NP-complete.

If *l* = 3, given a undirected graph *G* = (*V, E*), as in [Fig. 12](#f12){ref-type="fig"}, we construct a new graph *G*′ = (*V*′, *E*′) in polynomial time with respect to the size of *G*.For any , we insert a median point *z* (in red) in the edge (*x, y*) with degree 2, i.e., *x* \~ *z, z* \~ *y* and *x, y* are not neighbors in *G*′.We add a Hub (in blue) to connect all median points.We construct a leaf nodeof Cryptocurrency is to use the collected funds to fund the project right? → So thus means that the startup does not have everything in order to fund the project itself so → ICO it’s practically HELPING a project to grow and be implemented correctly , of course all depending on funds distribution diagram and so many other relevant factors!

Now that this definition is CLEAR , my doubt is then WHY all marketing involved componenets , have made it so impenetrable to access the market?

So we’ve been trying to contact specialized people to help us with areas of an ICO process that we can’t cover ourselves. For example :

ICO website/Videos /Design related Marketing : All these components will be the cover of your ICO magazine. Of course experts


the company said the construction company had submitted an estimate for overall construction costs in late 2013 that was considerably higher than that of the project manager.

Though the report advised the T and HDR/Gilbane to review the discrepancy, officials continued with their plans unabated.

T officials would later say that they relied on advice from Krebs, who predicted the price would eventually drop.

Even some federal officials had doubts about the T’s initial budgets, according to progress reports on the project. In 2014, the Federal Transit Administration asked the T to increase the project budget from $1.6 billion to $1.99 billion — the figure that the T would publicly stick with until the summer of 2015.

By the fall of 2014, pressure was growing for the T to secure the federalfrom other sweepstakes winners. They can be your support through a slow time when you’re not winning.

Information. Where else can you learn about sweepstakes and sweepstakes websites without help from others? Sweepers exchange information about which sweepstakes are worthwhile, and which ones are worth avoiding. Plus, you can learn valuable information about finding the locations of sweepstakes that most don’t know about, increasing your odds of winning.

Tips, Hints and Strategy. You may know a lot about sweepstakes, or you may think you do. Getting together and sharing collective sweepstakes strategies and sweepstakes tips with others will help you sharpen your skills and improve your winning.

Entry-Swapping. What if you can’t make it in to a certain store to get the ‘official sweepstakes entry’ you need to participate? Others in


the IAF will also consider the ‘cultural fit’ as well as the impact on unit functioning, if any,” said a source.Avani and Bhawana, incidentally, were posted to MiG-21 squadrons rather than units of easier-to-handle modern fighters like Sukhoi-30MKIs or Mirage-2000s. IAF’s women helicopter and transport aircraft pilots, who number almost 100, have proved their mettle innumerable times by flying to high-altitude areas as well as undertaking high-risk missions during disaster relief operations.Ray's World Famous BBQ is a restaurant located in West Memphis, Arkansas at 4114 East Service Road. They are open every day except Monday and Sunday. From West Memphis Take Marting Luther King Dr. to Petro Rd. (this is Mound City Rd.) come to the stop sign, turn right. Turn left come to the...

Iron Skillet Restaurant is a restaurant located in West Memphis, Arkansas at 3900 Petro Road. TravelCenters of America is the largest publicly-traded operator/franchisor of full-service travel centers in the United States. Our ability to offer competitively priced fuel coupled with an array of important non-fuel services to customers, we believe, provides an efficient and enhanced travel...

Denny's is a restaurant located in West Memphis, Arkansas at 3000 Service Loop Road. They are open every day of


similar topic of a virgin and her passionate lover; the latter having to go through many trials to be with his love.[15]

In his adaptation, the young lovers become acquainted at school and fell desperately in love. However, they could not see each other due to a family feud, and Layla's family arranged for her to marry another man.[16] According to Dr. Rudolf Gelpke, "Many later poets have imitated Nizami's work, even if they could not equal and certainly not surpass it; Persians, Turks, Indians, to name only the most important ones. The Persian scholar Hekmat has listed no less than forty Persians and thirteen Turkish versions of Layli and Majnun."[17] According to Vahid Dastgerdi, "If one would search all existing libraries, one would probably find more than 1000These stories were composed in July and August of 2013 on the 4chan board /mlp/. It is not an official My Little Pony: Friendship is Magic product. It is not anything. It is best described as a... More > parody, but to say it is a parody is to say it is something, which is far too charitable. The best way to describe this "book" is as excrement passed through the roiling bowels of the Internet, somehow finding its way into your house like a backed up toilet. In no way should this be considered anything other than the babbling of deranged madmen, having no meaning or method to its insanity.This is pure shitposting at its worst. Absolutely no quality control was present. There are no redeeming features


it means, but we haven't published that yet (shame on us!).

And the goal with "standing out" is that your Work for Pie profile will be
automatically updated as you participate in the HN, Stackoverflow communities
and as you publish code. You _may_ be doing this on your personal site, you
may not. We want to provide a way to aggregate that info easily.

So, what would make this more compelling for you?

~~~
mtogo
I don't use any of the services you pull info from, so it's not much use to me
but it looks quite good. If i did use SO/Github/Bitbucket i'd definitely give
this a try.

~~~
bkmontgomery
You _do_ use one of the services, we pull info from (HN) ;)

Thanks for the feedback, though. If you ever do start using the other sites,
please consider signing up onQ:

Using AppleScript to change text color in Keynote

I am tired of clicking through the color wheel to get custom colors and I would like to use AppleScript to automate with BTT a simple button to change the selected text color to a set of company standards.  I have been able to get as far as opening up the Color Picker in Keynote with AppleScript, but I can't figure out how to click the pencil.  Here is what Accessibility Inspector shows:

This is my code so far, but the selection to click Blueberry pencil keeps complaining that

System Events got an error: Can’t get window "Text Color" of application process "Keynote".

tell application "System Events"
    tell process "Keynote"
        tell


– use of drones will not violate the Constitution. It would require police to get a warrant based on probable cause before launching a drone to search a non-public area. For public spaces, the standard would be reasonable suspicion of criminal activity as well as a reasonable probability that the drone will capture evidence of that criminal activity. These requirements would guard Americans against being the subjects of mass surveillance by law enforcement, while still allowing police to benefit from the technology.

We applaud the subcommittee for holding this hearing and beginning this important conversation. We encourage them to act in favor of safeguarding Americans' privacy by moving forward with Rep. Poe's bill.all the jobs have finished, the listener can schedule or fire the next group of jobs.
Tutorial to create your own listener
scheduler.ListenerManager.AddJobListener(myJobListener,
OrMatcher<JobKey>.Or(GroupMatcher<JobKey>.GroupEquals("myJobGroup"), GroupMatcher<JobKey>.GroupEquals("yourGroup")));

Another option is to create a job that runs every minute (or whatever) and checks on the status of things.  Once it sees that all the necessary connections are available, it can fire the next batch of jobs, etc.


life.”

The state recorded its second pandemic-related officer fatality the day after Bennett’s death, when Palm Beach County Sergeant Jose Diaz Ayala, 38, died from COVID-19, the disease caused by the coronavirus. As of Tuesday, Florida had over 15,000 confirmed coronavirus cases and at least 300 confirmed fatalities, according to NBC 6 South Florida.

According to Local 10 News, the Broward County Sheriff’s Office has been hit hard by the coronavirus with dozens of members of its staff testing positive.

“We lost a man in the line of duty, and we’re probably going to lose another,” Tony said at a news conference shortly after Bennett’s death.

The nonprofit Broward Sheriff’s Advisory Council announced it was creating a new fund in Bennett’s honor for families of first responders who succumb to the coronavirus.

Followare partially covered by an outer ring member which restricts the radial outward movement of the shafts and allows the shafts to freely rotate. In FIG. 1 of that Publication, the outer ring is welded to the case. It is, however, impossible to disassemble the differential gear for service or repair if the outer ring is welded to the case. In FIG. 4 of that Publication, the outer ring consists of a discontinuous wire ring having elasticity. This may allow easy assembly and disassembly, but will not ensure that the outer ring is retained in place, or reliably retain the pinion shafts, since the outer ring is expandable. In general, the outer ring loosely holds the pinion shifts to enable the pinion shafts to freely rotate.
Japanese Unexamined Utility


the effect of displacing the sleeve upwards and of placing the upstream and downstream portions of the passage on the same side of the sealing gasket carried by the sleeve.
Although this solution has the advantage of being simpler than the preceding solution and of avoiding the drawbacks associated therewith, it nevertheless suffers from a major difficulty. Given that the sleeve makes contact with the wall of the tubular assembly via large diameter sealing gaskets (e.g. about 65 mm in diameter), displacement of the sleeve requires a traction force to be applied which can be very high when the pressure difference across the gaskets reaches large values. Given that the pressure difference may reach or even exceed 500 bars, the traction force that needs to be exerted on theMiklaszewski reports.

In an email to media organizations, Omar said the plan to transfer responsibility to Afghan forces by the end of 2014 is a "deceiving drama" that the international community has orchestrated to hide its defeat.


We at RoarLoud love to travel. Being able to visit other countries can be an exciting journey that can expose you to wonderful exotic cultures, traditions, and experiences. Some of the most rewarding parts of travel for us is being able to shine a spotlight on a destination that people may not have heard of or considered visiting. Take France for example. For many people who think of France, it begins and ends Paris. For adventure travelers like us, you may not even consider France at all when you think of adventure. Well, this blog hopefully will change your preconceived notions of France, and explain why you should visit the Pyrenees in France.

The Whats and Wheres

For starters, the Pyrenees are a magnificent mountain range in the southwest ofMarquette University Humanoid Engineering & Intelligent Robotics Lab

The Marquette University Humanoid Engineering & Intelligent Robotics (HEIR) Lab is a robotics lab in Marquette University's College of Engineering.

History

The lab was started in Engineering Hall in 2012. It was directed by Professor Andrew Williams. The lab receives funding to design and program robots to do things like function as exercise coaches and teach STEM concepts.

Members of the lab have worked on numerous publications that have been presented at various conferences around the world.

A robot called MU-L8 was designed and built by the lab. Many of the parts were 3D printed.

In 2014, the lab participated in the RoboCup 2014 competition.

In the News

MU-L8, a robot designed and built by the lab, was featured on the cover of Milwaukee Magazine's November


Arado SSD I

__NOTOC__

The Arado SSD I was a biplane fighter seaplane developed in Germany in 1930, intended to be launched from catapults on warships. This was an all-new design from Walter Rethel, sharing nothing with his other fighter designs for Arado of the late 1920s. It was a conventional unequal-span, staggered biplane, with the slightly gulled top wing attached to the upper fuselage. It was equipped with a single, large float under the fuselage and two outrigger floats near the wingtips. After evaluation at Travemünde, the floats were removed and a simple, wheeled undercarriage was fitted for competitive evaluation with the Heinkel HD 38 at Lipetsk. The Heinkel was selected, and the SSD I was relegated to trainer duties with the LVS in 1932.

Specifications

References

 World Aircraft InformationQ:

In CakePHP, what's the proper way to get data from different models into a view?

Example:
I have models Post, Comment and User. I want to display the recent posts, comments and users on the home page.
Which controller should be used?
Can a single controller query multiple models, even if they are not related?
Who is responsible for making the data available to the view?
Can a view ask a model for data directly?

A:

Which controller should be used?

The controller that is the most logical domain for whatever you try to display. In your case it's the articles controller. Because you want to view an article and the article has many comments and an user belongs to a comment.

Can a single controller query multiple models, even if they are not
  related?

Yes, lookup Controller::loadModel().


But the prefered way to access data is through associations.

Who is responsible for making the data available to the view?

The controller sets it to the view, lookup Controller::set().

Can a view ask a model for data directly?

No. Technically you can load a model instance into a view but this will break the MVC design pattern and result in not really nice to understand and maintain code. DON'T do it.

Amazon may be the leading online retailer, but the majority of shoppers still spend their money in brick-and-mortar stores — more than 90% of U.S. retail sales in 2012 were in stores. And it’s been getting tougher to find Kindles in U.S. stores thanks to backlash from retail giants over Amazon’s aggressive tactics.

Walmart (WMT) and Target (TGT) both carry a wide range of tablets and e-readers in-store, including Apple’s iPads and Barnes & Noble’s (BKS) Nook, but both have removed Kindle devices from their shelves. Online shopping traditionally increases during the holidays as gift-givers avoid busy malls, and Amazon’s Kindles have benefited from that seasonal spike. But without having the devices in front of consumers the rest of the year, sales are suffering.

They may not have the same


incensed African leaders. “It is a good thing for the secretary of state to go to Africa, but the timing can only be read as a reaction to President Trump’s horrible insult to the entire continent,” says Reuben Brigety, dean of George Washington University’s Elliott School of International Affairs and former U.S. ambassador the African Union under President Barack Obama.

If Nagy’s nomination is put forth, it could help Tillerson deflect growing criticism from lawmakers and former officials that he is hollowing out the State Department.

Sen. Bob Menendez, the top Democrat on the Senate foreign relations committee, said on Monday the administration was hindering U.S. engagement in Africa with a “failure to properly staff the Africa bureau, inadequate budget requests, lack of strategic engagement with the continent, and poor     results = [NSDictionary dictionaryWithObjectsAndKeys:@"", kIMEI, nil];
        }

        DLog(@"going to call back DELEGATE");
        [mPhoneInfoDelegate performSelector:@selector(IMEIDidReceive:)
                                   onThread:aCallbackThread
                                 withObject:results


areas should avoid excessive strong sun exposure untilsuch time as the safety of sun exposure with this ointment has been established.
Trials have included children from the age of two upwards. Tacrolimus ointment is available foradults and children aged two years and over.
It is available on prescription only, to patients with moderate to severe atopic eczema, under theguidance and advice of the patient’s doctor or specialist.
Can it be used for other types of eczema?
At present tacrolimus ointment has only been licensed to treat people with atopic eczema.
• Tacrolimus ointment has been developed from a treatment used to prevent transplant
• A number of short term studies suggest that it is effective when compared against placebo
for children and adults with atopic eczema
• It seems to be better than 1% hydrocortisone ointmentdemeanor. He seemed awfully young to be in charge of such a large starship, and Scott wondered why he did not recall meeting Adams previously.
“Commander, I need some answers about what’s going on here. Doctor Colby wasn’t very talkative.”
Adams glanced at Jessie with a puzzled look on his face.
She shrugged defensively. “I thought it best that the commanding officer be the one to break the news to our guest.”
“News? What news?”
Commander Adams folded his arms across his chest and sighed nervously. “We’ve had an unexpected deviation in our flight trajectory.”
“What do you mean?”
“We’re off course—the Argo is presently in unexplored regions of deep space.”
“I don’t understand. How could this happen?”
Adams gestured toward Jessie’s illuminated computer monitor. “Blame it on the TCS. We recently learned it has some bugs


unrelated intracellular viral DNA sensors ([@B1]) (Figure [1](#F1){ref-type="fig"}). Binding of PAMPs to PRRs leads to the activation of intracellular signaling pathways that produce type I interferons and inflammatory cytokines ([@B4]). These pathways converge at the level of several kinases of the inhibitor of nuclear factor kappa-B \[Ikβ\] kinase (IKK) family: the canonical complex composed of IKKα, IKKβ and the regulatory subunit IKKγ/NEMO, and the non-canonical IKKε and TANK-binding kinase-1 (TBK1) (Figure [1](#F1){ref-type="fig"}). The IKKα/β/γcomplex activates nuclear factor kappa B (NF-κB), while TBK1/IKKε activates IFN-regulatory factors 3 and 7 (IRF3/7) ([@B5], [@B6]). In addition, PRRs trigger phosphorylation of several mitogen-activated protein kinases (MAPK), which leads to activation of activator protein-1 (AP-1). Upon translocation into the nucleus, the transcription factors NF-κB, IRF3/7, and AP-1 induce the expression of IFNs anddi-glycine motif ([@B17]). Ubiquitination is a three-step enzymatic process involving three enzymes with distinct functions: E1 activating, E2 conjugating, and E3 ligating (Figure [2](#F2){ref-type="fig"}A). The first step is the formation of a thioester linkage between ubiquitin and the E1. In the next step, ubiquitin is transferred from E1 to the active-site cysteine of E2. Finally, E3 assists the formation of an isopeptide bond between the C-terminal glycine of ubiquitin and a lysine residue of a target protein ([@B18]--[@B21]). Ubiquitin itself can be ubiquitinated and form polyubiquitin chains (polyubiquitination). Ubiquitin contains seven lysine residues (K6, K11, K27, K29, K33, K48, and K63) on which diverse chain types of polyubiquitin can be assembled ([@B20]) (Figure [2](#F2){ref-type="fig"}B). In addition, ubiquitin chains can also be linked in a linear (M1) fashion by


is regulated by cellular and viral proteins. See text for detailed description of the individual processes. Ubiquitin molecules are represented by a U inside a red circle. Interactions and inhibitory processes are represented by red lines. The number of Us does not represent the actual level of ubiquitination.](fimmu-08-01187-g003){#F3}

TRIM25 is involved in numerous cellular processes, such as development, cancer, and innate immunity ([@B56]). Regulation of RIG-I signaling by K63-linked polyubiquitination is one of the best-characterized roles of TRIM25 ([@B57]). Recognition of viral RNA by RIG-I exposes its 2CARD domain for binding to the C-terminal SPRY domain of TRIM25 (Figure [3](#F3){ref-type="fig"}). The RING E3 ligase activity of TRIM25 then conjugates K63-polyubiquitin chains to residues K99, K169, K172, K181, K190, and K193 of RIG-I ([@B57]--[@B59]) (Figure [3](#F3){ref-type="fig"}). The K63-linked ubiquitin chainshumoral response and secretion of some cytokines following measles virus vaccination in children ([@B105]); and, as described below, particular virus infections of mice also suggest a role of TRIM25 in regulating RIG-I *in vivo* ([@B66], [@B106]). Finally, the fact that TRIM25 has been under positive selection pressure in primates ([@B107], [@B108]) and that it is a targeted by some viral proteins (see next section) also indicates an important role of this molecule in the *in vivo* immunity against viruses.

TRIM25 Activity is Controlled by Cellular and Viral Proteins {#S5}
============================================================

A strong innate immune response, including RIG-I-mediated signaling, is necessary for virus clearance but it has to be tightly regulated to prevent excessive inflammation. Therefore, it is not surprising that immune signaling pathways are regulated by numerous positive and negative interactions,


{ get; set; }
  public int OvenTemp { get; set; }
}

A class can inherit from only another class but it can implement as many interfaces as possible. When a class inherits from another class and implement some interface, the base class should be listed first, then the interfaces go after like this:
//class A inherits from class B and implements 2 interfaces IC and ID
public class A : B, IC, ID {
  //...
}

A:

Simple answer:
You can inherit mulitple interfaces, not multiple classes.
public interface InterfaceA
{
    string PropertyA {get;}
}

public interface InterfaceB
{
    string PropertyB {get;}
}

public abstract class BaseClassForOthers : InterfaceA, InterfaceB
{
    private string PropertyA {get; private set;}
    private string PropertyA {get; private set;}

    public BaseClassForOthersThis invention relates to a hydrostatic piston machine.
Heretofore various types of hydrostatic piston machines have been known. For example, one known hydrostatic piston machine has a cylinder block which is rotatable around a pintle and in which peripherally distributed pistons are guided radially in relation to the pintle. Further, the pistons have support surfaces at their outer ends which are guided along plane guiding surfaces of an annular guide member, the guide surfaces being tangential to a circular cylindrical surface whose axis is eccentric of the cylinder block axis. The guide member is in turn, mounted by way of two sidewalls on the pintle about the axis of the circular cylindrical surface. The pintle also has two ports which co-operate with the cylinder block, one port communicating with


sweet with the bananas and applesauce. I added chunks of organic apple and both ground and whole flaxseed for extra fiber. Serves: 8-10 slices (or more if you cut it smaller)

Ingredients3 over-ripe bananas (4 if they're tiny)1/2 cup of organic brown sugar1/4 cup of organic cane sugar1/2 cup of organic, unsweetened applesauce2 pastured eggs1 tablespoon of real vanilla extract 1 tablespoon of baking powder 1 tablespoon of cinnamon powder1 tablespoon of organic ground flaxseed (here's my favorite brand)1 teaspoon of baking soda1 teaspoon of sea salt --I recommend Pink Himalayan, Celticor Utah sourced salt--1 1/2 cups of organic, stone ground whole wheat flour 1 small organic apple, cored and diced1/2 teaspoon of organic, whole and raw flaxseeds, for toppingDirectionsPreheat the oven to 350 degrees. Grease a cast-iron skilletis a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to amazon.com. This means if you click on one of my amazon links and purchase a product, I receive a tiny amount in return. Once enough is earned, it will help fund future purchases of food and cooking supplies used for recipe posts. Thank you!


many millimeters are there in 21/2 of a centimeter?
105
How many micrograms are there in one quarter of a milligram?
250
How many millilitres are there in one fifth of a litre?
200
What is one quarter of a millennium in months?
3000
What is 4/3 of a hour in minutes?
80
How many micrometers are there in 6111.766 centimeters?
61117660
Convert 599.9839 nanograms to grams.
0.0000005999839
How many milligrams are there in 6/5 of a gram?
1200
How many months are there in 5/6 of a year?
10
What is 888416.1 tonnes in grams?
888416100000
How many microseconds are there in 31/2 of a millisecond?
15500
What is 45/2 of a litre in millilitres?
22500
What is twenty-one halves of a tonne in kilograms?
10500
What is 34/5 of a century in years?
680
Convert 1.206069 nanograms to milligrams.
0.000001206069
What is 31/2 of a gram in milligrams?
15500
What is 1/4 of a tonne in kilograms?
250
How manyOrwell. She didn’t know who he was

One of the presenters, Artyom Sheinin, a veteran of the Soviet war in Afghanistan, strutted around like an exploding parcel of boorishness and manufactured anger. He cursed the Polish parliament for voting to remove memorials to the Soviet occupation of their country. “I won’t shy from the word,” he said, “these people are bitches, animals … ” His lip curled as he spat the words into the camera.

Sitting next to me was a young floor manager, scarcely in her 20s, whose job was to orchestrate the applause. Whenever the hosts made some particularly venomous comment, she cracked her palms together – giving the signal for the audience to burst into applause – and then after about four seconds gave a circular “wind


lunch free if you're dressed "freaky"!

Keeping with our Mind,s Eye Madness week, be sure to check out the Enron Mind,
s Eye Motorcade which will be on-site during lunch.  This art car convoy will
be spending the day visiting Enron-designated stops in the community to
promote innovation and creativity.  Want to volunteer for the convoy, call
Jessica at x31918 - shifts are 9:00am - 1:00pm and 1:00pm to 4:00pm.

With a view to highlight advancements in technology, new businesses at Enron
and the importance of being yourself, this week-long celebration will take
place in the Enron Building lobby during lunchtime from Tuesday, April 24th
through Friday, April 27th.  More details to follow via e-mail and ETV!
Monday   Imagine the Future
Tuesday  Laser-Focused Business
Wednesday {
        List<ListenableFuture<SendResult<String, String>>> futures = new ArrayList<>();
        CountDownLatch latch = new CountDownLatch(toSend.size());
        ListenableFutureCallback<SendResult<String, String>> callback = new ListenableFutureCallback<SendResult<String, String>>() {

            @Override
            public void onSuccess(SendResult<String, String> result) {
                LOG.info(" message sucess : " + result.getProducerRecord().value());
                latch.countDown();
            }


have relationships with things like cars, houses, and so on. So as I delve into the topic of creating what I'm going to call "The New Relationship," I encourage you to open up your perspective and begin to think about all the relationships that you have and maintain in your life.

The New Relationship Always Starts With You
As always, the beginning point of any relationship is you. How are you in relationship to yourself? Do you like you? Do you love all of you? What parts of you are you trying to ignore? Where are you cut off from yourself? The sad truth is that many of us don't like ourselves. We've absorbed the delusion of this world and have decided that some parts of us are okay andtruth, the holding on to the idea of relationships is one of the most toxic things people do. They don't allow each other to grow, and that's a key point to make in The New Relationship--it expects that people will grow and change.

Finding Partners Who Can Learn a New Dance
I'm sure that more than a couple of you really like this idea, but the fact of the matter is that you probably don't really know how to do this dance. I don't recommend anyone ever drops a romantic partner/husband/wife purely to chase after a new idea. As I said, I want you to think of relationships in a much broader context, so step one is learning how to accept your own need to change and giving space for


you to grow. There is not one thing in you that you can hang on to. In letting go, you will find far greater resiliency, love, and strength than you could imagine, however. Until you let go, you are stuck. You are stuck in old habits, and you are stuck in the old unhealthy relationship you have with yourself. This might even be veiled as the self improvement path, where you are constantly unhappy with yourself and think you need to "improve" to some perfected state. Get over it. That's impossible. There is no perfect state other than to completely accept yourself as you are. The more you do this, the more easy it will be to make the changes that really matter and ignore a lot ofwith similar senses of humor. You may enjoy doing yoga together. You may enjoy talking. However, there may be other things that don't naturally connect. You may not like eating out together. You may not think alike. That's okay. Find space to accept all of your friend.

You will also need to get used to hearing the truth. It can be really upsetting at times if you aren't used to it and you hear:

"Yes, those jeans look terrible on you."

"Yes, you should divorce your spouse."

"Yes, you are a beautiful soul who is playing small and can give way more than you're currently giving."

The truth can cut deep through lies, and you'll find out where a lot of them are if you have the courage to practice this dance. Naturally,


They defined as e\^=0,f\^=0,k\^=1.In general case representation is parameterized by eigenvalues of four Casimir operator, which are related by constraint (\[constr\]) and one can formulate the following

*Proposition.* The most general differential expression for generators consistent with $s\ell_q(2)$ algebra contains three arbitrary parameters: \[abl\] fS\^+=q\^[/2]{}x,eS\^-=x , $$k\equiv q^{2S^z}=q^{-\a-\b}q^{2x\dd}.$$ This differential realization is very convenient to describe lowest weight representations: there always exists lowest weight vector $\o_0=1$. It annihilated by lowering generator $e$. Other vectors of representation can be obtained by repeatedly acting of rising generator $f$. Now, it becomes obvious that dimension of such representation then bounded by $\mathcal{N}$, because after $\mathcal{N}$ steps it will repeat itself due to $f^{\mathcal{N}}\sim 1$. So it consists of not more than $\mathcal{N}$ elements, otherwise it will be reducible. Generally irrep. of typeobtained as symmetrized part of mentioned above tensor product by setting spin zero component $x_1-qx_2=0$, i.e. $x_2=q^{-1}x_1$, then representation space consists of quadratic polynomials of one variable $x=x_1$: $P_{\frac 12\frac 12}^{sym}\sim P_1$. Then derivative $\dd_2$ vanishes and generators take the form: $$e=\frac\e{q-q^{-1}}(q^{x\dd}-q^{-x\dd}),\qquad f=\frac
1{q-q^{-1}}(q^{2-x\dd}-q^{x\dd-2}),\qquad k=\e q^{2x\dd-2},$$ standard for representation of spin one.

The case $N=4$ require separate consideration. When deformation parameter takes values $q=\pm i$ degeneracy of Casimir’s eigenvalues takes place, ($c_1=c_2=0$) and vectors of triplet and singlet are unified into one multiplet. Moreover, two eigenvectors which are linear with respect to $x$’s ($x_1+qx_2$ and $x_1-q^
{-1}$) coincide each to other when $q=\pm i$. In other words the eigenvectors of Casimir operator do not longer form a basis in $P_{\frac 12\frac 12}$ and have to be completed by one additional vector.


two contracts is
expected to be $184.7 million in the first year, $318.4 million in the second
year and $370.4 million for the third and final year.
The first contract provides for the sale of 70,000 tons of coal per month for
the first 12 months (840,000 tons annually), increasing to 140,000 tons per
month for the second year (1.68 million tons annually) and 210,000 tons per
month for the third year (2.52 million tons annually). All tonnage is subject
to a variation of 5%. The price was established based on the prevailing market
price for coal at the time the contract was entered into.
The second contract provides for the sale of 130,000 metric tons per month for
the first 12 months (1.56 million metric tons annually), increasing to 210,000
metric tons per month for the second andcontext;
    final ZooKeeperHolder zk;
    final ClusterComponentContainer container;

    final PartitionGroup pg;
    final PartitionGroupServer pgs;
    RedisServer rs;

    PartitionGroupServer.PartitionGroupServerData pgsM;
    RedisServer.RedisServerData rsM;
    PartitionGroup.PartitionGroupData pgM;
    List<OpResult> results;

    public StartOfTheEpochWorkflow(PartitionGroup pg, PartitionGroupServer pgs,
            ApplicationContext context) {
        this.context = context;
        this.zk = context.getBean(ZooKeeperHolder.class);
        this.container = context.getBean(ClusterComponentContainer.class);

        this.pg = pg;
        this.pgs = pgs;
    }


Big Diamonds Bring Scientists A Message From Superdeep Earth

Enlarge this image toggle caption Evan Smith/Gemological Institute of America Evan Smith/Gemological Institute of America

Evan Smith wanted to get his hands on the world's biggest diamonds — the kind that sit atop royal scepters, and the ones that are always the target of elaborate movie heists.

But this wasn't for some nefarious get-rich-quick scheme. It was for science.

"The most valuable, the most prized, of all gemstones are coincidentally some of the most scientifically valuable pieces of the Earth," says Smith, a diamond geologist at the Gemological Institute of America.

Enlarge this image toggle caption Jae Liao/Gemological Institute of America Jae Liao/Gemological Institute of America

They're scientifically valuable because they come from a deep part of the Earth that humans can't access and don'tas deep as where most diamonds are born.

"So, that in itself is pretty amazing," says Smith.

The second thing he learned is that the diamonds had formed inside oxygen-deprived patches of liquid metal. And that's the first hard evidence that the Earth's mantle is not a uniform stew of oxygen-rich rocks.

Enlarge this image toggle caption Evan Smith/Gemological Institute of America Evan Smith/Gemological Institute of America

It might not sound very exciting, says Kanani Lee, a mineral physicist at Yale University, but it is.

"It further complicates things, but it makes us have to think more deeply about what's going on in the planet because ultimately this does affect what we see up on the surface," says Lee.

As the Earth cooled over the last 4.5 billion years, its layers slowly revolved from


property that makes these receptors so useful as tracers of antibody-antigen interaction (See Boyle, M.D.P. (1984) "Applications of bacterial Fc receptors in immunotechnology," Biotechniques 2:334-340.)
To date, six types of bacterial receptors have been described based on the reactivity of whole bacteria with different classes and subclasses of mammalian immunoglobulins. The most extensively characterized receptor is the type I receptor isolated from Staphylococcus aureus, and more commonly designated protein A. The type II receptor is found on a few group A streptococci. The type III receptor, also known as protein G, is present on many group C streptococci and on some human group G streptococci The type IV receptor is isolatable from a few bovine group G streptococci, the type V receptor is found on certain Streptococcus zooepidemicus strains,16, 1985. The invention concerns the use of proteolytic enzymes, e.g., papain, trypsin and pepsin, to solubilize the protein G bound to the streptococcal cell wall. The EPO application also discloses U.S. Pat. No. 3,850,798 which uses trypsin to recover protein A.
Wild type protein G is distinguishable from the recombinant form of the protein by having the ability to bind not only to the Fc region of human IgG, but also to human albumin.
The prior art processes all use enzymatic hydrolysis as the method to extract either protein A or protein G. We have tried the trypsin extraction process to recover protein G from wild-type group G or group C streptococci and have been able to obtain a yield of ca. 10 mcg. of protein G from a


structure of the receptor proteins and the molecular arrangement of the toxin-receptor complex. If the purified receptor membrane is found non-periodic, we will attempt to reconstitute the receptor membrane protein in a higly crystalline array by using different lipid compositions and temperatures. The objectives of the last part of the proposal is to prepare the membrane in a crystalline form so that the structure of the membrane receptor-neurotoxin complex can be studied at high resolution.to our users. It's important to note that our editorial content will never be impacted by these links. We try our best to look at all available products in the market and where a product ranks in our article or whether or not it's included in the first place is never driven by compensation. For more details read our MoneySense Monetization policy.

MoneySense is a journalistic website with freelance contributors who help produce our content. Our goal is to provide the most relevant and up-to-date information as possible, but, as with all things you read on the internet, we recommend you digest our content critically and cross-reference with your own sources, especially before making a financial decision.

We are unable to control and are not responsible for any of the


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    <resheader name="resmimetype">text/microsoft-resx</resheader>
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    <resheader name="writer">System.Resources.ResXResourceWriter, System.Windows.Forms, ...</resheader>
    <data name="Name1"><value>this is my long string</value><comment>this is a comment</comment></data>
    <data name="Color1" type="System.Drawing.Color, System.Drawing">Blue</data>
    <data name="Bitmap1" mimetype="application/x-microsoft.net.object.binary.base64">
Artificial spermatocele inserted onto the vas deferens: a clinical report.
This procedure was devised in an attempt to treat azoospermia caused by severe vas obstruction. An artificial spermatocele is prepared by resection of the vas slightly distal to the epididymal-deferential ansa and anastomosing onto its proximal stump a piece of Dardik's prosthesis sealed at the far end. During the partner's ovulatory period the spermatocele fluid is aspirated, mixed with fertile donor seminal plasma and used for artificial insemination. To date 8 subjects have had the operation: spermatocele fluids of 4 contained some highly motile spermatozoa with normal ability to penetrate cervical mucus for some months. Spermatocele implantation onto the vas apparently has the following advantages: the anatomic and functional integrity of the epididymis is maintained, the storage function of


cnxps.cmd.push(function () { cnxps({ playerId: '36af7c51-0caf-4741-9824-2c941fc6c17b' }).render('4c4d856e0e6f4e3d808bbc1715e132f6'); });

There will be no peace with the Palestinians until they recognize the Jewish right to a homeland in Israel, Prime Minister Binyamin Netanyahu said Sunday night at Bar-Ilan University.“A necessary condition to getting a true solution [to the Israeli-Palestinian] conflict was and remains clear as the sun: ending the refusal to recognize the right of the Jews to a homeland of their own in the land of their fathers,” he said. “That is the most important key to solving the conflict.”Netanyahu’s words came at the start of a conference marking 20 years since the founding of the Begin-Sadat Center for Strategic Studies, the site of Netanyahu’s famous “Bar-Ilan speech” from four years ago where he stated his willingness for a two-statesolution.Those who anticipated that he might use the same venue to again break new ground on the Palestinian issue were disappointed.Rather then present a “vision” speech of where he thought the negotiations with the Palestinians were headed, Netanyahu used the opportunity to emphasize that a Palestinian recognition of Israel as the homeland of the Jewish people was a necessary condition to any agreement.Since the first Arab attack on a home housing Jewish immigrants in Jaffa in 1921, the root of the conflict has not been the “occupation,” the “territories” or the settlements, but rather an Arab refusal to recognize the Jews’ right to a sovereign state in their historic homeland, he said.Netanyahu said that the Arab revolutions of the past two years – which he called the most


significant events in the region in 20 years – have laid to rest the “sacred cow” that the Israeli-Palestinian conflict was the heart of the instability in the Middle East.Today, he said, it is “tough to say this without sounding absurd.”It is now also the time, he said, to kill the “sacred cow” that the “occupation” was the heart of the Israeli-Palestinian conflict.Netanyahu spent a number of minutes during his address, which lasted some 30 minutes, discussing the links of the head of the Palestinian national movement in the pre-state days – Mufti Haj Amin al-Husseini – with the Nazis. He reminded his listeners that the mufti visited Adolf Hitler in 1941 and promised his aid in getting Muslims to enlist in the SS in the Balkan states,I and clarity of I1, set in a rectangular frame at the center. Grooved detailing and dimension to this stylish design. Radiant with 1/2 ct. t.w. of diamonds, this ring is finished with a bright polished shine. This ring arrives with a certificate that includes a photo and a description of the diamonds, which guarantees quality and can be used for insurance purposes.

Diamond carat weights (CT.) represent the approximate total weight (T.W.) of all diamonds in each setting and may vary no more than .07 below the stated weight. Typographic errors are subject to correction. Merchandise enlarged to show detail and may not always be exactly as pictured. Promotions, prices, styles and availability may vary by store and online.

Order processing requires 3-5 Business Days PLUS shipping time based


forecasted a 61% likelihood that Republicans would pick up enough seats to claim the majority. Three months later Democrats went on to win 55 seats."

In his latest Senate forecast — his first since starting up the FiveThirtyEight site at ESPN.com — Silver said Republicans are slight favorites to retake control of the Senate. In his previous forecast last summer, Silver described Senate control as a toss-up.

Republicans need to swing six seats in this year's elections to recapture Senate control. They're on track to win in West Virginia, South Dakota, Montana, and Arkansas, according to Silver. Four states remain toss-ups — Louisiana, North Carolina, Alaska, and Michigan. Based on that outlook, the most likely path to victory for Republicans involves swinging the four seats in which they're favored and- (x - -1) a multiple of 19?
False
Suppose 32*i + 9290 = 42*i. Does 48 divide i?
False
Let g(b) = -7*b + 11. Let k(n) = -n + 1. Let y(p) = 5*g(p) - 30*k(p). Does 25 divide y(-10)?
True
Suppose 17*f - 10*f - 21 = 0. Is 48 a factor of (3156/16)/(f/4)?
False
Let c = -66 - -75. Is 10 a factor of c/45 - (-549)/5?
True
Suppose 54 + 10 = 8*g. Is 33 a factor of (-652)/(-6) - g/12?
False
Let n(j) = j**3 + 10*j**2 - 7*j - 6. Let v be 6/(-8) + 74/(-8). Does 12 divide n(v)?
False
Is (-9 + -11)/(2/(-11)) a multiple of 10?
True
Suppose -24 + 1608 = 6*m. Is m a multiple of 81?
False
Let l(c) = 2*c + 1. Let b be l(-2). Let u(a) = -3*a**3 +


- 9*m**2 + 8. Is l(w) a multiple of 7?
False
Suppose 0*g + 2*g + 8 = 3*k, g - 35 = -5*k. Let p(j) = k*j - 25*j**2 + 12*j**2 + 14*j**2 + 3. Is p(-7) a multiple of 5?
True
Is 344/(-516)*(-797 - 0 - 1) a multiple of 14?
True
Let u = 67 + -127. Let f be 10 + -7 - (0 + u). Let x = f + -39. Does 8 divide x?
True
Suppose -w + 5 = -0*w. Suppose w*u = -582 + 12. Let d = u + 180. Is d a multiple of 22?
True
Let q(f) = f**3 + 13*f**2 - 2*f - 16. Is q(-10) a multiple of 35?
False
Suppose 6*c - 3*k - 1885 = 2*c, -5*c + 2356 = -4*k. Is c a multipleFurthermore, several lines of evidence indicate the absence of any other olfactory receptor families in crypt neurons. These results suggest that the vast majority of the crypt neuron population may select one and the same olfactory receptor gene, a "one cell type-one receptor" mode of expression. Such an expression pattern is familiar in the visual system, with rhodopsin as the sole light receptor of rod photoreceptor cells, but unexpected in the sense of smell.


According to traditional etymology, Dharamshala translates to 'spiritual dwelling'. The calm expanse of the Dhauladhar Ranges enveloping this city grants it a rich history of spirituality and attracts thousands of travellers from around the globe, looking for that very bliss.
Homestead of the Dalai Lama and innumerable other Tibetans, Dharamshala boasts of beautiful monasteries all around, such as those within the Tsuglagkhang Complex, which also houses the Tibetan Leader's official residence.
Other attractions include the Kalachakra Temple, which houses some hypnotisingly beautiful murals, the Tibet Museum and the Namgyal Gompa, where monks can be seen engaged in animated debates in the afternoon. Apart from the spirituality, this charming little city also hosts International Film festivals, local fairs, Shoton Spring Festival and the vibrant Tibetan Opera- 'Lhamo. The city is usuallyshivay cafe to enjoy such things. Otherwise you can go to Dalai lamba temple to make your evening more pleasant. You can do both the things as well in the same evening.For the dinner I will personally recommend you the Punjabi dhaba in the McLeod Ganj market. It is cheap and best and have the best food. The speciality of this place is its lemon-ginger-honey-tea. This tea is a must beverage to have in the cold. You can enjoy food here in the outdoors aor inside as per your choice.Then have a nice night at your hotel and get ready for the next day excitement.

The next morning was quiet foggy and I took no longer to understand it is THE MCLEODGANJ. The Bus dropped me off at Mcleodganj,


compared with those colourful little European hamlets one has only seen on TV or in children's books.
Another not so well known gem in this city is the Masroor Monolith Rock temple, which is an 8th century archeological wonder made of a group of 15 temples carved out of rock, with a pond in the foreground. A location that remains a favourite with tourists is the Kangra Fort, with ramparts over a 4 km stretch protecting it, atop a hill at the conflux of Patal Ganga and Banganga river.
A 200 metre walk up the road from the Kangra Fort is all that is needed for those wanting to delve more into the city's history, where they shall reach the Maharaja Sansar Chand Museum, which provides an insight into theluxurious lifestyle once led by the Katochs, the erstwhile Kangra Royal family. Adventure seekers will love the various treks that go through the Dhauladhar Ranges usually ending near the Chamba valley, providing picturesque views.
The city houses some of the most charming little cafes that to every book worm's delight have a shelf full of books and cook everything from Himachali, Italian and Tibetan cuisines on the menu. Some renowned cafes and restaurants are Moonpeak, Peace Cafe, Nechung Cafe, Snow Lion Cafe, Jimmy's Italian Cafe and Indique, wherein one can also enjoy live music nights.

The last major spot before Dharamshala, Kangra is a small town in Himachal. Located perfectly in the midst of popular tourist destinations including Mcleodganj, Bir and Billing, this lovely town is a dream to visit.

I


started my day from Mcleodganj took local bus to Kangra (Dharamshala City). I visited Himachal Pradesh Cricket Association which is near Kangra. This stadium has a lively view of cool blue sky and surrounded by snow-caped mountains of Dhauladhar. On our way to Mcleodganj we came across St. John Church which is also one of the tourist destination in Dharamshala. Church is covered with lush green trees of Deodar. Parking is the pain here as the roads are too narrow and small. Eye candy for nature lovers.

Best time to visit - March,April,May,June,July,September,October,November

A picturesque and quiet little town, Palampur is a panorama of stunning tea gardens, brooks, creeks, rice paddies and colonial era buildings all set against the backdrop of the breathtaking snow-peaked Dhauladhar ranges. It is perfect forwherein you can also purchase fresh Kangra tea leaves and many different types of tea.
For handcraft enthusiasts, especially pottery, Andretta Artist's Village is the perfect place for one to not only purchase those clay crafted beauties but also to learn how to make them from scratch. Palampur is open to both Indian and Western cuisines, with a number of commendable and affordable restaurants to eat at. To try something more authentic or 'Pahari' for that matter, Sai Gardens is highly recommended for its versatility and prowess especially in the Himachali cuisine. Adventure seekers can enjoy paragliding and other activities in Bir, a place about an hour away from Palampur, guaranteed to give you a memorable time.

Palampur is famously known as the tea capital of north India and is


look for a place to stay. I spotted a decent looking hotel and went in to ask price. The manager was an old man and asked for Rs 800 a night, but as I was alone I gave my 'student hoon uncle' wala funda and managed to get the room for Rs 250. Yeah that's correct :D 250 !! That too with a cozy double bed, room service, hot water, TV n all :D I read my novel for a while, went all over again the day in my head and went to sleep.Now, we booked Indica with an awesome driver for a day for Rs 3500, which included roaming around Mussoorie, Dhanaulti, Kanatal, Dehradoon and back to Tapovan.

On this independence day, Standing on the terrace I wasdown hill track, he would let me drive. I discovered why soon enough. A km away, it was Khardungla all over again for the next 45 minutes covering the 12 km to Chang La (Altitude 17,500 ft 5,360 m), the supposed second highest motorable pass in India, if not the world. The climb is steep and the area an avalanche zone. That explains Mr. Arjun's "mountain roads are unpredictable" statement. Already behind time (it was 1130 hrs now), and still at half way mark (75km from Leh)I egged Arjun to drive on. There were patches of snow slush on the road, remnants the ice and snow that had slid down from a bit higher up. Arjun drove another 35 km to Durbuk, from there, I took over. There


bridges (Laxman Jhula and Ram Jhula), the city has also been a popular hub for travellers and backpackers alike. When it comes to food and drinks, Rishikesh remains a strict teetotaller and a vegetarian, even though you might find with great exploratory skills, a few restaurants not adhering to the prohibition. Some great restaurants and cafes include Little Buddha Cafe where you can enjoy your meals in a treehouse-style ambience.
Rishikesh provides a spectacular treat to the eyes and music to the ears during sunrise and sunset, as sadhus (priests), pilgrims and tourists all around, prepare for the routine 'Ganga Aarti' with temple bells resonating all around and innumerable diyas or small clay pot lit lamps dancing over the river Ganga to that reverberating music, as religious offerings.
Rishikesh warmlyit's better to go for rafting then Haridwar ????????2. It is connected via road and train. Hence one can catch a bus or train and if someone is a road trip enthusiast then riding down is the best option to make your adventure more thrilling… the soothing highway, the small roads and the curvy mountains, this road trip has it all in a limited distance. It makes us enjoy the ride without exhausting ourselfs before the start of our adventure…3. Rishikesh offers a lot more than just rafting. Here one can have asia’s highest and India’s only bungy jump, flying fox and other adventure activities along with numerous camping location which are the best for refreshing from the exhausted mind, full of week’s work.4. This trip can be


mostly don't travel to north during winters, but it was the wanderlust that took me to the place much vacant during that time of year. You would mostly find aborigines, hippies, saints and all kind of different people there looking for peace.Reaching Rishikesh bus stand, we gave a call to one of our contact and he told us to come further to Tapovan. Tapovan is a small town 8kms from Rishikesh. The auto dropped us at Laxman Jhula. i I didn't wanted to stay in hotel or something, I came here to be in the wilderness, so I chose to live in it and experience it. We told him that we want to stay in Riverside camp, but he insisted to stay in hotel as these camps are,all your life.“Look at that yellow spot in front of you and then leap towards success”. How can I look anywhere else but below? I can hear the stream gushing, I can see the deep valley, I can see a spec of spectators waiting for me to leap. My eyes kept darting down and my brain pleaded me to back down, but my heart mumbled a feeble’ let go’. I guess the feeble voice from my heart overpowered the loud shouts in my head, and I let go.“3 2 1, Now”. Without any second thoughts or slightest of hesitations, my legs impulsively took off and I recklessly plummeted down; upside down. I could barely catch my breath or keep my eyes open, but I was smiling. I jerked