Mafia. "So the best way to penetrate that closed inner circle is by flipping people, and flipping them up."

After investigators get a sense of which players are part of a criminal enterprise, they start by targeting those at the lowest levels.

"If they don't voluntarily cooperate, you get honest leverage on them to compel their cooperation," said Patrick Cotter, a former federal prosecutor who was part of the team that convicted the Gambino crime family boss John Gotti in the 1990s. "You find their criminal conduct and use that to force them to do what they should have done originally, which is to tell the truth."

Former Trump campaign chairman Paul Manafort pleaded guilty this week. Mark Wilson/Getty Images

Honig said he once nailed a case by flipping someone who wasthe driver for a more powerful person in the organization.

"That led us right up the chain," he said. "And you can see that happening in the Russia investigation."

The first plea deal Mueller's office announced was that of George Papadopoulos, who served as an early foreign policy aide to the Trump campaign. Next, he looped in Michael Flynn, the former national security adviser who admitted to lying to the FBI.

In February, Rick Gates, the former deputy chairman of the Trump campaign, announced that he would be pleading guilty and cooperating with the special counsel. Gates' cooperation led prosecutors upstream, and his courtroom testimony against Manafort helped them successfully convict his former boss on eight counts of financial fraud last month.

Likewise, legal scholars say, Manafort's cooperation, as well as that


harvested to investigate whether incubating cells in these SPMs moderated the expression of markers of the proinflammatory phenotype of diseased tendon stromal cells and potentiated expression of SPM synthetic enzymes and receptors mediating resolution of inflammation. The concentration and integrity of mediators used for these incubations were validated using UV spectrophotometry and liquid chromatography with tandem mass spectrometry in accordance with published criteria.[@bib17] Bioactive LM profiling of media and lysate samples from IL-1β--stimulated tendon cells was performed using a previously described method.[@bib15] Calibration curves were obtained for each using authentic compound mixtures and deuterium labeled LM at 0.78, 1.56, 3.12, 6.25, 12.5, 25, 50, 100, and 200 pg. Linear calibration curves were obtained for each LM, which gave *r*^2^ values of 0.98 to 0.99.

Immunocytochemistry for LXB4- and RvE1-TreatedI will provide the time and attention into each individual case whether it is a family law or criminal case so that my clients can be assured that their case is being competently handled.

I am a member of the California State Bar since 2006; the San Bernardino County Bar and Association of Certified Family Law Specialist.
I am a certified Family Specialist (I am the only female Certified Family Law Specialist in the Cities of San Bernardino, Redlands and Highland).
For the past 6 years I have had the Indigent Defense Contract with the County of San Bernardino wherein I was court appointed to represent several hundred of defendants who were charged with Contempt of Court for violation of court orders (typically non-payment of child support). I also represented several


Court and the United States Central District Court of California. His primary area of practice is Family Law.

Attorney Ruiz started college at age 17 and graduated from CSU, Los Angeles with honors and Dean's List recognition in 2000. She graduated from Loyola Law School of Los Angeles in May of 2004 and passed the Bar exam in July of 2004. During the last 13 years, she has gained experience with family law cases such as domestic violence, divorce, legal separation, paternity, government child support, step-parent adoption, guardianship and grandparent visitation.

She primarily assist the families of the San Bernardino and Riverside counties. In Los Angeles County, Attorney Ruiz attends Court in the East District...

Matthew J. Knez graduated from the University of Redlands in California where he earned a Bachelor10:00 p.m.
The proofs further show that approximately 10 minutes after defendant was arrested for operating his motor vehicle while under the influence of intoxicating liquor, Officer Macchio learned of Dunay's knowledge of the earlier events, at least to the extent that Dunay had observed defendant scraping his license plate on the curb. Dunay had given a statement concerning his observations to Officer McLaughlin. His testimony at trial, however, exceeded the breadth of the information contained in that statement. Apparently, the written statement did not refer to the fact that Dunay observed defendant park the second time and then "[get] out of the car and [walk] across to [him]" or that he saw defendant "staggering." At approximately 10:20 p.m., armed with knowledge of Dunay's observation of defendant's operation of


that that charge included his driving the motor vehicle to the place where he parked it on the sidewalk and was subsequently arrested. Defendant received Dunay's written statement through pretrial discovery and was well aware prior to the municipal court trial of the essence of Dunay's possible testimony, to wit, that he [defendant] was operating his motor vehicle in an erratic manner on the evening of October 7, 1983, shortly prior to 10:00 p.m. when he was arrested. Moreover, defendant's untimely motion to suppress Dunay's testimony as the fruit of the unlawful arrest belies any argument now by defendant that he did not know he was being charged and tried for "operating" his motor vehicle while under the influence of intoxicating liquor on the evening of October 7,191 N.W.2d 799, 801 (N.D.Sup. *408 Ct. 1971); Colling v. Hjelle, 125 N.W.2d 453, 456-457 (N.D.Sup. Ct. 1964); Schutt v. MacDuff, 205 Misc. 43, 127 N.Y.S.2d 116, 127 (Sup.Ct. 1954). Cf. State v. Wetherell, 82 Wash.2d 865, 514 P.2d 1069, 1073 (Sup.Ct. 1973). But see Glass v. Commonwealth, Dept. of Transp., Bur. of T.S., 460 Pa. 362, 333 A.2d 768, 770-771 (1975).
In Gallagher v. Michigan Sec. of State, supra, for example, the Michigan Court of Appeals held that a valid arrest was an absolute condition precedent under a Michigan "implied consent" statute to the right of a law enforcement officer to request a person to submit to chemical tests of his blood, breath, urine or other bodily substances or suffer the penalty for such refusal, stating:
We are acutely


you gentlemen entered into a stipulation in this matter?
PROSECUTOR: Yes, Your Honor. I have written it down for purpose of Mr. Melli and I to be able to see that it was as complete as we wanted it to be. And the stipulation is this as I understand it.
PROSECUTOR: That all elements necessary to establish a prima facie case by the State are conceded and stipulated to by the defendant other than operation. The defendant will waive the necessity of the State proving other than operation to the extent that the State must prove items such as identity, condition and alcoholic influence. Also, it is waived or it is conceded for the same purpose as to the proof of the prima facie case that  to the extentFaithful." The second chapter of the book of Matthew details how the three wise men worshiped Jesus at the time of his birth: "And when they were come into the house, they saw the young child with Mary his mother, and fell down, and worshiped him."

"Yes, Jesus loves me. Yes, Jesus loves me. Yes, Jesus..."

Loves me

Is the king

Heals me

Died for me

In Paul the Apostle's epistle to the Ephesians, he stresses the undeniable love of Jesus. The third chapter and nineteenth verse of the book of Ephesians reads, "And to know the love of Christ, which passeth knowledge, that ye might be filled with all the fullness of God."

"His truth is..."

The right way

Marching on

The glory

Here to stay

This very popular chorus line is taken from the song titled "Battle Hymn


of the Republic." Julia Ward Howe wrote the song.

"His faithful follower I would be. For by His hand he..."

Made me whole

Healed my soul

Leadeth me

Saved me

It is highly probable that the third verse in the twenty-third chapter of the book of Psalms inspired this chorus line taken from the worship song titled "He Leadeth Me." The Psalms verse goes, "He restoreth my soul: he leadeth me in the paths of righteousness for his name's sake."

"Hallelujah! Thine the glory! Revive..."

By and by

This church

Us again

The saints

Willam P. Mackay wrote the song that has this chorus line. The name of the song is "Revive Us Again."

"Lord of all, to Thee we raise this our hymn of grateful..."

Honor

Salutations

Thanks

Praise

The seventh chapter of the second book of Samuel provides a clear reason why God deserveshill cannot be hid."

"I will cling to the old rugged cross, And exchange it some day for..."

A crown

A ticket to Glory

Forgiveness

A lily

The third chapter of the book of John at the sixteenth verse famously states: "For God so loved the world, that he gave his only begotten Son, that whosoever believeth in him should not perish, but have everlasting life." The chorus line mentioned here is taken from the worship song "The Old Rugged Cross," which reflects the salvation principle stated in the book of John.

"I'd rather have Jesus than..."

The riches of the world

Anything this world affords today

Sinful things

Worldly possessions

The sixteenth chapter of the book of Matthew poses the question, "For what is a man profited, if he shall gain the whole world, and lose his own soul?"


level games, and 6mm battalion level games. Each is very different in level of detail/abstraction and certain mechanics are very different but in spirit and in general procedures they're similar.

For example, the 28/15mm platoon level sci-fi version must handle everything from giant alien bugs to basic human troopers to robots and cyborgs using a common data structure. For this version we use 40K-like stat lines for troops and weapons.

Meanwhile, the 20/15mm company level, near future version is more abstract with its focus on conventional forces and no need to handle Bugs vs Colonial Marines. For this version we use a single reference sheet along the lines of old school games. Technically, vehicles are still defined in a separate sheet but in broad terms. So, once you know thatPennsylvania somapitt. When intensity of exposed stimuli and the stimulative value of experi- mental settings are not below or beyond the average level, although this level, only a small fraction will proceed into the spinal dorsal horn, while the rest is transported rostrally with the cerebrospinal fluid.

(2001). a Type bond n-Type bond (d) FIG. 18) is the principal carbohydrate in milk and is of critical nutritional importance to mammals in the early stages of their lives. Dut- ton, 1975. Immunoglobulin light chain class multiplicity and alternative organizational forms in early vertebrate phylogeny. Cement is a mixture of a combination of lime (CaO), silica (SiO2) and alumina (Al2O3), which sets when mixed with water.

Cloning offspring from adults with desirable traits has been successfully performed on cattle, goats, mice, cats,


the posterior tibial nerve, with tarsal tunnel syndrome: the Heel Pain Triad [13].

Reconfiguring databases Although the ISBN itself is changing to 13 digits, the Book Industry Study Group recommends that the product identifier in all databases be expanded to 14 digits, rather than to only 13. PPOCT may potentially measure the excited state absorption spectrum by wavelength scanning the pump andor the probe radiation.Abstract (in English)

Under new heritage legislation in South Africa the
conservation of ‘place’ and inherent intangible values
have been enshrined, but are not readily supported by
environmental controlling bodies and developers in
practice. Three case studies are used to demonstrate how
intangible place values were sucessfully defended where
developmental and political pressures indicated their loss.
The first example explains the process of identifying a
suitable site and providing urban design guidelines for
the introduction of 500 low-cost housing units in a ‘mixed’
cultural property that is earmarked for nomination for
World Heritage status – namely, the gold-rush town of
Pilgrim’s Rest and the Blyde River Canyon - with the aim
of retaining the intangible heritage resources whilst
redressing historical race-based injustices and providing
much-needed economic stimulus for the heritage place.
In the second example the intangible values inherent to
the Union Buildings in Pretoria -


Extramedullary hemopoiesis arising in the gut mimicking carcinoma of the cecum.
A patient initially treated for iron deficiency anemia was later found to have myelofibrosis. She developed increasing splenomegaly and then a mass in the right iliac fossa for which a right hemicolectomy was performed. Histopathology demonstrated extramedullary hemopoiesis in the spleen and cecum. The patient subsequently developed a protein-losing enteropathy and she deteriorated and died 14 mths later. Autopsy was not performed.column of equally potent sentences to do full justice
to the building which rears its handsome façade upon Albert-square.
(Brisbane Courier 4 May 1915: 5)

When it opened, the Tivoli was unique in Australia for combining two theatres
in the one building; the design allowed for two separate companies to function
autonomously. The building permitted distinct entrances for the audiences
of the two separate theatres. One of McIntosh’s publicity boasts at the opening
of the new theatre was that:

“It is built on what is termed the
intimate plan,” said Mr. McIntosh; “every seat is close enough to the stage
for the occupant to thoroughly appreciate every word and every expression
of the artists. The lighting is all on what is known as the indirect.’ When
complete there will not be one light to shine direct in the eyes, the


Any Tag warrants further comment. This option offers some very useful tags that you can insert into your report. There are over 40 different tags that you can insert, from a simple button to additional components such as an applet. There is some documentation available on each of the tags but I would look to find detailed information about each of the options and what they do from a more Web-based documentation site such as a professional Web design site. For example, the ability to use some applets is still available in the BI 7.0 version, but you need to be careful with the coding required to make them work with XHTML versus HTML. There can be differences in the different object tags being used between the twoveterinary care. A total of 9% of canine and 32% of feline samples tested positive for the presence of *C. ureolyticus*. Metagenomic studies carried out on cats, report percentages of *Campylobacter* spp. as high as 10% \[[@B19]\]. Moreover, *Campylobacter* spp. of potential clinical relevance, other than *C. jejuni* and *C. coli*, such as *C. upsaliensis*, have been shown to be present in dog faeces \[[@B6]\]. PCR detection rates of *Campylobacter* spp. from dog faeces are highly variable (58--97%) depending on the study, geographical location and methodology used \[[@B20]\].

Various factors appear to influence the level of *Campylobacter* spp. faecal shedding in cats and dogs. These include the age of the animal, with puppies \< 6 months and cats \<36 months more frequently positive for *Campylobacter* spp. \[[@B21]-[@B23]\]; housing conditions, with dogs housed in


general, may also be a attributed to the presence of organisms in a viable but nonculturable state. It has been shown that campylobacter cells which enter this state, cannot be cultured on artificial media, despite remaining virulent and capable of invading intestinal cells \[[@B32]\].

Molecular based detection offers an alternative, allowing us to circumvent the problems facing traditional culture based approaches. Therefore, despite a failure to grow pathogenic *Campylobacter* spp., their detection with molecular methods is still likely to be of clinical significance.

Conclusion
==========

The effect of *C. ureolyticus* infections on animal health has, to date, not been investigated in any great detail. All stools samples collected throughout this study, were non-diarrhoeal, therefore no comparison of *C. ureolyticus* rates in healthy and sick animals was possible.

Nevertheless, this is the first studyher first ever magazine cover!" Raking huge amonunt of Moolah at the box office, 'Gully Boy' entered the prestigious Rs 100 crore club in eight days. Even before the release of the film in India, Zoya Akhtar's 'Gully Boy' garnered immense accolades in the coveted Berlin Film Festival.

Now, with the recently released 'Made in Heaven' Zoya made her debut in the digital space as well. The Zoya Akhtar and Reema Kagti creation traces the journey of two wedding planners and unveils the scandalous reality behind the big fat Indian weddings. Zoya Akhtar who has been ahead of her times with films 'Luck By Chance', 'Zindagi Na Milegi Dobara' and 'Dil Dhadakne Do' is now ruling the box office with 'Gully Boy' and is making all right noise across


T. rubrum, but in fingernails C. albicans seems to be at least as important as T. rubrum. On the trunk (mainly the chest, especially in women) and buttocks C. albicans was again the main etiologic agent for fungal infections. Dermatophytes accounted for only 32.4% and 14.2% of the fungal infections on the buttock and trunk, respectively. On the buttock and trunk T. rubrum was the main etiologic agent as far as dermatophytes are concerned. On the trunk, Microsporum canis and M. ferrugineum were of some importance as well. The prevalence of tinea capitis was very low and accounted for only 0.7% of all fungal infections. Cutaneous candidosis accounted for 30.3% of all fungal infections and is therefore important. On the chest, buttocks, groin and finger-nails, the prevalence of2007, the Daily Times in Salisbury Maryland, reported that at least 25 patients received unnecessary stent procedures performed by Dr John McLean, at Peninsula Regional Medical Center in 2006, and said the hospital is seeking to determine whether any more patients are at risk.

According to the Times, the hospital’s initial probe found that many of patients did not meet the minimum 70% blockage guideline used to determine whether a stent is needed.

The hospital said, “it would rectify the situation by paying back full medical fees to patients and their health insurance providers for the procedure.”

Paying back the cost of the device and procedure will do nothing to calm the worry of patients who must now live out their lives wondering whether they will develop a blood clot each


Press.

The first DES was approved in April 2003, and by December 2006, at a hearing on the “Prospective on Drug‑eluting Stents: Balancing Risks and Benefits,” an FDA advisory panel reported that 3 out of 5 procedures were for unapproved uses and DES patients were developing stent-thrombosis, or blood clots, occurring most often in patients when the devices were used off-label.

While testifying at the hearing, Dr Sanjay Kaul told the panel that bare-metal stents were hardly used anymore. “The current drug‑eluting stent utilization rate,” he said, “is about 80 percent, and we have learned that nearly 60 percent of it is off-label.”

“We’ve also learned from the off-label use,” he informed the panel, “that the risk of stent thrombosis is higher, 2 to 3 percent, and in some subsets, likeinsulin-requiring diabetics, as high as 5 to 6 percent.”

As a result of the massive off-label use of DES, by 2005, the device accounted for 43% of total sales for Boston and 52% for J&J, according to Bloomberg on December 4, 2006. By 2006, sales figures show the new stent was a $2 billion seller for Boston.

The stents have been implanted in more than 6 million people worldwide, in what market analysts are calling a modern day record for a new device. In the January/February 2006 issue, Medical Device Link reported that about 1.5 million patients in the US were implanted with stents in 2005.

Yet by end of 2006, Forbes was reporting a study that found that angioplasty and stents did not help patients when used within a few


boost sales of its blockbuster anti-anemia drug Procrit by offering contracts that fattened doctors’ profits and urging its salespeople to push higher-than-approved doses.”

This bit of news came shortly after federal lawmakers ordered J&J to cease all direct-to-consumer advertising and physician incentives for Procrit until the FDA could determine whether steps needed to be taken to protect the public following investigations that revealed the rampant off-label sale of the anemia drug was causing serious injuries and death among kidney and cancer patients.

Unfortunately, there is no way to medically reverse the stenting procedure and therefore, the millions of unsuspecting patients who received the DES face a life-time of every day worry because a blot clot lodged in a stent can cause a stroke or heart attack without any warning.

Legal expertstheir approval only tested the stents in patients with single, smaller artery blockages, according to the December 8, 2006 Science Daily.

“But doctors quickly started using them,” Science reports, “in patients with blockages in multiple vessels and in those with larger blockages or co-existing diseases like diabetes.”

Stents are tiny wire-mesh tubes inserted in arteries to keep them open after angioplasty, a procedure in which a balloon is threaded through a vessel in the groin to a blocked artery and then inflated to unclog the artery to restore blood flow to the heart.

The use of stents became popular because the procedure was promoted as being safer and cheaper then heart bypass surgery in which an entire section of a diseased artery is replaced with a vessel taken from elsewhere in


the “Prospective on Drug‑eluting Stents: Balancing Risks and Benefits,” an FDA advisory panel reported that 3 out of 5 procedures were for unapproved uses and DES patients were developing stent-thrombosis, or blood clots, occurring most often in patients when the devices were used off-label.

While testifying at the hearing, Dr Sanjay Kaul told the panel that bare-metal stents were hardly used anymore. “The current drug‑eluting stent utilization rate,” he said, “is about 80 percent, and we have learned that nearly 60 percent of it is off-label.”

“We’ve also learned from the off-label use,” he informed the panel, “that the risk of stent thrombosis is higher, 2 to 3 percent, and in some subsets, like insulin-requiring diabetics, as high as 5 to 6 percent.”

As a result of the massive off-label useproduct that supposedly showed few risks in company sponsored trials with a small number of patients, actually causes serious health problems that went undetected until after the device was implanted in millions of people in large part due to the massive off label marketing of the device the minute it was FDA approved.

But in this instance, the off-label sale of the DES has resulted in a public health crisis because the adverse events are lethal. A clot inside a stent can instantly cut off the blood supply to the heart and patients can not have the device removed because tissue grows over the stent once it is placed in the artery. And taking Plavix for life, if it even helps, creates another life-long health threat.

The fact is, there


is no medical solution to this problem and the millions of patients implanted with the stents who are lucky enough not to experience an adverse event will still have to endure a lifetime of day to day worry and dread.

Feds Investigate Profits From Off-Label Stent Procedures – Part II

In addition to the federal investigations into the off-label marketing of drug eluting stent by Boston Scientific and Johnson & Johnson, on May 10, 2007, Rep Maurice Hinchey (D-NY), who serves on the House Appropriations Subcommittee, announced the introduction of the FDA reform bill which addresses the issue of doctors using products for unapproved uses, which usually occurs without the patient’s knowledge or consent.

If a doctor does not inform a patient that the FDA has not approved the use ofarteries.

Many stable heart patients are conned into stenting because they believe that it will extend their lives and lower their risks for heart attack but according to the New York Times, the COURAGE study found that patients who received stents and drugs had the same life expectancy and same number of heart attacks as patients who received drugs only.

The study reported the rate of heart attack, stroke or death in patients who received stents was 20%, compared to 19.5% in patients who used drugs alone. At the end of 4.6 years, there were 211 deaths, or 19% among patients in the group who received stenting compared with to only 202 deaths, or 18.5% in the medication group.

“Our findings parallel those reported in recent trials,” said William Boden, chief


professor of medicine and director of cardiology at McMaster University in Hamilton, Ontario, said that he plans to ask interventional cardiologists at his hospital to re-evaluate their use of drug-eluting stents so that they are used “in a limited way” until more data are available to determine whether the stents are “a panacea – as many of our patients and residents believe – or an expensive placebo, or – as I suspect – a Trojan horse.”

Dr Yusuf says the stents may be more of a Trojan horse, carrying hidden long-term danger in the guise of a useful therapy to avoid restenosis. As it is now, Dr Yusuf characterized the widespread use of drug eluting stents as “madness” and said that coronary interventions should not be used to treatidea and drive to actually finish and release this thing started taking shape, and as soon as I had the fonts chosen and everything in place I was sold on publishing it. I am a perfectionist, so I spent way too many hours on this book cover (the paperback version being the most difficult by a long shot) but with the help of some friends for advice and cheering me on (looking at you, dear Rachael!)—it got done.

Rachael: You did a great job with it! The fact that you wrote this novella, designed the cover, and published it all by yourself makes me think you may be some kind of superwoman! Aww! I loved watching the changes being made and the cover edited to perfection. It’s definitely my


worst, second is the best.

Rachael: You are just too good at this! 😉 I never know what to expect when I ask random questions and this response was PERFECT!

Meghan: Rachael thank you so very much for having me! ((BIG HUG)) Hope you all have had a merry Christmas and a blessed, joy-filled 2017!

Rachael: Anytime, dearest Meghan! I can’t wait to have you back here again! *VIRTUAL HUG!* Hopefully I can see you, soon!

Thank you, readers, for joining us! If you are interested in buying Meghan’s wonderful book, you can find it here:and the Center for American Progress.

The EPA, of course, is getting in on the act, taking small farms as “buffer zones” for waterways to protect them from pesticides. Under Agenda 21, these areas are designated as not inhabitable by humans, as detailed by the Wildlands Project.

EPA Administrator Lisa Jackson is on the Rural Council, which was established, according to President Obama, “to strengthen rural communities and promote economic growth.”


another - but the message did not get through.

Share this article

He said: 'Unfortunately, although the instruction to not grit that section on route 17 was given, the instruction that route 18 would grit that section was not confirmed.

'Therefore from the 29th October the route had not been gritted.'

And a tracker fitted to one of the gritting trucks, which could have told if the road was being tackled, was not working, the Norwich hearing was told.

A police investigation found Miss Wooler lost control as her car swerved to the right and hit a tree stump, sending it spinning into three trees.

Sorry: Council bosses have made a grovelling apology to the 27-year-old's family after bungling gritters failed to salt an icy road. Ms Wooler was driving to work when she[The status of NIHS Information and Computing Infrastructure (NICI)].
From 1999 to 2000, NIHS Information and Computing Infrastructure (NICI) were newly renovated. The purposes of the renovation are (1) the improvement of the communication for business works in NIHS, (2) supporting for the research, (3) supporting for the administration work. The Internet connection speed was upgraded from 256 Kbps to 1.5 Mbps. The high quality network sever machines and database server machines were installed. The large-scale software systems were renewed their versions. Four experimental stations for medical plants at Hokkaido, Izu, Wakayama and Tanegashima connected to NIHS at Tokyo or Osaka branch by ISDN. We describe the providing information on NIHS home page, and how to utilize NICI for our research and official works.


Background
==========

Intrauterine adhesions (IUAs), also acquainted as Asherman syndrome (AS), arise most frequently following infection or trauma \[[@b1-medscimonit-25-4094]\]. It results in amenorrhea, infertility, and various complications of pregnancy, including preterm premature rupture of membranes, malpresentation, and placental abruption \[[@b2-medscimonit-25-4094],[@b3-medscimonit-25-4094]\]. In addition, IUAs can potentially impair the blood supply to the uterus and early fetus \[[@b4-medscimonit-25-4094],[@b5-medscimonit-25-4094]\]. These clinical symptoms are associated with major health concerns, particularly for women of childbearing age. In recent years, IUAs has become the second most common cause of female infertility due to the increasing use of uterine cavity surgery \[[@b6-medscimonit-25-4094]\]. Although it is widely accepted that the disease is caused by acute inflammatory and dysregulation of transcription factors \[[@b7-medscimonit-25-4094],[@b8-medscimonit-25-4094]\], the precise pathological mechanisms involved remain unclear.

MicroRNAs (miRNAs) are a family of small, endogenous, non-coding, singleof the National Institutes of Health. The protocol was approved by the Ethics Committee of Taian City Central Hospital. Female rats weighting 260--280 g were purchased from CAMS (Chinese Academy of Medical Sciences, Shanghai, China). The model was performed as previously described \[[@b7-medscimonit-25-4094]\].

Bioinformatics prediction for target genes
------------------------------------------

The target genes of miR-466 were predicted using online software, including [miRNA.org](http://miRNA.org) and TargetScan 7.1.

EGFP reporter assay
-------------------

Cells were seeded in 48-well plates 1 day before transfection, and then co-transfected with pri-miR-466 or Anti-miR-466 and pEGFP-NUS1 3′UTR, pEGFP-NUS13′UTR mut. The vector pDsRed2-N1 (Clontech, 632406), which expresses RFP, was included for transfection normalization. After transfection for 48 h, the cells were lysed using radio-immunoprecipitation assay (RIPA) lysis buffer (Sigma, R0278), and the EGFP and RFP intensities were measured with a fluorescence spectrophotometer (Hitachi, F4500).

MTT


assay
---------

H8 and End1/E6E7 cells were seeded in 96-well plates at 5000 cells per well 1 day prior to transfection. Cell viability was determined by MTT assay. Then, 10 μl of 5mg/ml MTT (Sigma, 298931) was added and incubated for 6 h. The medium was aspirated and MTT was dissolved in 100 μl DMSO (Sigma, 67685) and absorbance was read at 570 nm using a Quant Universal microplate spectrophotometer.

Transwell migration and invasion assay
--------------------------------------

Cell migration and invasion ability was evaluated by a Transwell assay, as described previously. Briefly, 6×10^5^ H8 and End1/E6E7 cells in 200 μl of serum-free medium were cultured in a chamber containing an 8-μm polycarbonate filter (Millipore, USA) coated with 30 μl of Matrigel (BD) for the invasion assay. After incubating for 48 h, cells remaining on3B](#f3-medscimonit-25-4094){ref-type="fig"}). Then, we constructed the overexpression and knockdown vectors of miR-466 and validated its efficiencies in H8 and End1/E6E7 cells ([Figure 3C](#f3-medscimonit-25-4094){ref-type="fig"}). Co-transfection with miR-466 and the NUS1 3′UTR reporter significantly decreased EGFP intensities, while we found increased EGFP intensities by co-transfection with ASO-miR-466 and the NUS1 3′UTR reporter. However, after co-transfection pri-miR-466 or ASO-miR-466 with the mutated 3′UTR reporter, the EGFP intensities were unchanged ([Figure 3D, 3E](#f3-medscimonit-25-4094){ref-type="fig"}). As shown in [Figure 3F and 3G](#f3-medscimonit-25-4094){ref-type="fig"}, the mRNA and protein levels of NUS1 were significantly reduced by transfection miR-466 expression vector compared with the empty vector control in H8 and End1/E6E7 cells ([Figure 3F, 3G](#f3-medscimonit-25-4094){ref-type="fig"}).

miR-466 inhibited cell proliferation and EMT process *in vitro* and *in vivo* by regulating NUS1
------------------------------------------------------------------------------------------------

MTT analysis and colony formation analysis showed that miR-466 inhibited the


for anti-doping.

To bring the issue to the world’s attention, we enlisted Ben Johnson, the world’s most famous drugs cheat, on the 25th anniversary of his infamous Seoul 1988 Olympics 100m final. We took Ben on a global tour to spread the message, share his life experience, garner support and deliver a petition to the IOC headquarters in Lausanne, Switzerland.

The tour generated over £50m of global earned media. But more importantly, 12 weeks after the tour and delivery of the petition, IOC president, Thomas Bach announceda $10m increase in funding to the World Anti-Doping Agency and a further $10m fund “to protect the clean athlete from any kind of manipulation or related corruption”.\tilde r (\tilde{h}-\tilde{e})^3 \frac{\partial}{\partial \tilde r}\left( \tilde {h}+ \tilde \kappa \right)\right)-\frac{\mathcal{\tilde E}}{(\tilde{h}-\tilde{e})}=0 \ ,
\label{lubrication_equation}$$

with an evaporation number $\mathcal{\tilde E}$ defined by

$$\label{evap_number}
\mathcal{\tilde E}=\frac{\lambda_v \mu_v \Delta T}{\gamma_d \rho_v \ell_{c_d} \mathcal{L}} \ .$$

As for the curvature $\tilde \kappa$, it is here left as a full-form expression

$$\tilde \kappa=\frac{\frac{\partial^2 \tilde {h}}{\partial \tilde r^2}+\frac{1}{\tilde r} \left(1+\left({\frac{\partial \tilde {h}}{\partial \tilde r}}\right)^2\right)\frac{\partial \tilde {h}}{\partial \tilde r}}{\left(1+{\left(\frac{\partial \tilde {h}}{\partial \tilde r}\right)}^2\right)^{3/2}} \ ,
\label{curvature}$$

with no small-slope simplifications applied. The reason is that such a full expression proves to eventually provide for a smoother numerical matching with the upper part of the drop. Appart from the size $\tilde R$ of the drop, $\mathcal{\tilde E}$ is an other parameter of the dimensionless problem and depends on the fluid properties and on the superheat $\Delta T=T_p-T_{\rm sat}$. While the liquid properties


of the pool and form a liquid lens. However, if the temperature of the pool is above $T_{\rm sat}$, a drop like the one shown in Fig. \[figures/photoFlo\] can be observed. This figure shows an ethanol drop of $1.2$ mm in radius levitating over a pool of silicone oil V20 at $T_p =79^\circ$C. Erythrosine has been added to the ethanol for a better visualisation. We clearly distinguish that the drop stands above the deformed surface of a liquid pool and keeps an almost spherical shape. For larger drops, the deformation of the pool surface is observed to be more significant. Drop radii up to $5$ mm could be experimentally reached. However, for such large drops, even a small perturbation induces their contact with the pool. On the other hand,of Leidenfrost drop evaporating on a solid substrate follows the power law

$$R  (t) = R_0  \left( 1- \frac{t}{\tau} \right) ^\alpha \ ,
\label{eq:RVst_general}$$

![image](Evaporation.pdf){width="\textwidth"}

where $R_0$ is the initial radius of the drop, $\tau$ is the drop characteristic lifetime and $\alpha$ is an exponent depending on the shape of the drop. For a drop with a radius larger than the capillary length $\ell_{c_d}$ (puddle shape), $\alpha = 2$, and for drop with a radius smaller than $\ell_{c_d}$ (quasi-spherical shape), $\alpha = 0.5$. In our case of a Leidenfrost drop evaporating above a deformable substrate, the data still appear to be well fitted by Eq.(\[eq:RVst\_general\]). However, $\alpha = 1$ here whatever the value of the drop radius. Indeed, one sees in Fig. \[figures:RVst\_Ethanol\] that the drop radius appears to decrease linearly


the contact leads to a liquid lens that is very quickly evaporated.

Further considerations {#sec:considerations}
======================

The presented model actually treats the substrate as an ideal deformable substrate, *i.e.* the pool is assumed to be isothermal and no flow develops inside. As a consequence, the influence of the pool viscosity and thermal properties is not considered in the model. Therefore, even if our model appears to rather correctly capture the main mechanisms of the phenomenon, it has inherent limitations which will be discused in this section. The only properties of the pool accounted for in the model are its density and surface tension, whose influence will also be investigated.

Effects of density and surface tension ratios {#sec:Density/SurfaceTension}
---------------------------------------------

Figures \[fig:DensitySurfaceTension\] present the results of a parametric study highlighting the role of the ratios of the{
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Night Together" (Mick Jagger, Keith Richards) – 3:40
"Mississippi Moon" (Peter Rowan) – 3:06
"Midnight Town" (Robert Hunter, John Kahn) – 3:12
The album was reissued in the All Good Things: Jerry Garcia Studio Sessions box set with the following bonus tracks:
<li>"That's a Touch I Like" (Jesse Winchester) – 3:40
<li>"(I'm a) Road Runner" (Brian Holland, Lamont Dozier, Eddie Holland) – 4:10
<li>"It's Too Late (She's Gone)" (Chuck Willis) – 4:27
<li>"I'll Forget You" (copyright control) – 3:21
<li>"Tragedy" (Fred Burch, Gerald Nelson) – 3:52
<li>"Think" (Deadric Malone, Jimmy McKracklin) – 4:12
<li>"I Know It's a Sin" (Jimmy Reed) – 2:41
<li>"Lonesome Town" (Thomas Baker Knight) – 6:19
<li>"Cardiac Arrest (Studio Jam)" (Garcia, Kahn, Michael Omartian, Merl Saunders, Ron Tutt) – 1:39
<li>"Back Home in Indiana" (James Hanley, Ballard McDonald) – 7:08

Personnel

Musicians
Jerry Garcia – guitar, vocals, classical guitar
Arthur Adams –margin very sparsely ciliate with few scattered short hairs; corolla-lobes 4, reflexed, twisted apically, white with orange-brown elongated-dots, lanceolate, 6.4--7 × 1.7--2.3 mm, glabrous on both surfaces; stamens 4, spreading upright (in open flower), filament ±sessile, anthers twisted apically, lanceolate, ca. 5--6.2 × 0.8 mm, apex elongated into a hyaline tip, thecae open by longitudinal-slits, with scattered, lineate, orange brown dots behind, glabrous; ovary ovoid, ca. 7 × 6 mm, style and stigma filiform, ca. 6.8 mm long, ovules ca. 6 in 1-series. *Fruits* n.v.

### Distribution.

Endemic to Borneo; known only from Sungai Mentaya, Kotawaringin Timur, Central Kalimantan.

### Etymology.

The species epithet commemorates Graham Charles George Argent (1941--2019), a prominent botanist on tropical botany in South-East Asia and a leading expert on Ericaceae, especially the tropical 'Vireya' rhododendrons, and collector


as UPC/EAN, Code 39, etc.; 1D stacked bar code symbologies, Code 49, PDF417, etc.; and two-dimensional (2D) data matrix symbologies.
One-dimensional (1D) optical bar code readers are well known in the art. Examples of such readers include readers of the Metrologic Voyager® Series Laser Scanner manufactured by Metrologic Instruments, Inc. Such readers include processing circuits that are able to read one dimensional (1D) linear bar code symbologies, such as the UPC/EAN code, Code 39, etc., that are widely used in supermarkets. Such 1D linear symbologies are characterized by data that is encoded along a single axis, in the widths of bars and spaces, so that such symbols can be read from a single scan along that axis, provided that the symbol is imaged with a sufficiently high resolution alongdiplomacy’: “I always went to cocktail parties, for two reasons: one, to see and be seen, and two, as the Romans say, in vino veritas — truth comes out over a glass.”

David Edwards, who spent three decades in the foreign service and retired in 2011, says he hasn’t heard much waxing on about “dithering dandies” in recent years: “People are actually on the frontlines, a lot of people have been shot at, we have people in Baghdad, in and out of Libya, Haiti…I think it has moved from glamour to grit.”

Former Canadian Ambassador to Ukraine Abbie Dann, who retired in 2013 after 33 years in the foreign service, likens being a foreign service officer to a calling, such as serving in the military, or feeling compelled to become


a doctor or a human rights lawyer. “It’s not even a profession,” she said, “it should be considered a vocation. I’m Catholic, so I can use words like that.”

“I have lost 20 percent of my lung capacity, from pollution in Sao Paulo, Bombay and Kiev. And I've never smoked — lots of us are like that. Real foreign service officers are brought up a bit like the army: don’t explain, don’t complain, just get it done,” Dann said.

Michael Kologie, outgoing president of the Professional Association of Foreign Service Officers (PAFSO), agrees, and points out that the officers themselves are often not the only ones making sacrifices. “As diplomats, we have to remember that we are not just working from 8:30am-6pm — we’re representing Canada abroad 24/7. And it’snot just foreign service officers doing it, it’s their families that are doing it.”

One of the main purposes of the foreign service is to provide fearless advice and loyal implementation when it comes to the government of the day’s foreign policy, which necessitates close cooperation with Canada’s foreign ministers.

Lloyd Axworthy, who served as minister of foreign affairs from 1996-2000, says that when the Chrétien government was working on developing an ambitious treaty to ban landmines, Canadian embassy staff around the world “worked the streets,” in diplomatic parlance, with the goal of pulling off a treaty at the UN.

Thanks to the foreign embassy network set up under Pearson in the post-war years, Canada was able to draw on infrastructure in every region: “There have always been [those] in the


Treasury Board saying oh, why do we need embassies in Patagonia, or something, and I said well, because they vote at the UN, they’ve got interests, and we never know when we’re going to need them.” In Axworthy’s opinion, the strength and ability of the foreign service has been one of the reasons why Canada has been able to play, when it wants to, an effective role on the international stage.

Barbara McDougall, Canada’s secretary of state for external affairs from 1991-1993, offers a more mixed assessment.

“There’s no question in my mind that the foreign service is the most professional of all the public service…That doesn’t mean that I thought they were always on top of their files,” she told OpenCanada.

In 1992, McDougall was the first Canadian foreign ministerHarper is not being repaired,” he added. “That, I think, is an area really deserving of investigation.”

So, what have been the lasting effects of the Harper years? After almost a decade of cuts, has funding been restored? Has morale? And what gets lost when Canada isn’t playing at full capacity on the battlegrounds of diplomacy?


feet.

Jump Descriptions

Our jumps are divided into 2 categories: starter jumps & advanced jumps. In order to qualify for an advanced jump, you must first demonstrate proper technique and awareness for 2 starter jumps. This is to ensure your safety and best possible experience.

Beginner Jumps

Superman Front Dive:

While wearing the Full Body Harness you will stand on the jump platform facing forward off the bridge. You will then dive forward just as if you were diving into a swimming pool.

Backward Plunge:

While wearing the Full Body Harness you will stand on the jump platform facing your friends on the bridge. You will then crouch down on the platform as if you were hanging onto the wall of a swimming pool and preparing to launch backwards into the water.

Advanced Jumps

Backward Lumber Jack:

While*/
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** and 'double' (the default), 32-bit integers and 'float' (for
** restricted platforms), and 'long'/'double' (for C compilers not
** compliant with C99, which may not have support for 'long long').
*/

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#define LUA_INT_LONG		2
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#define LUA_INT_TYPE	LUA_INT_LONG
#endif
#define LUA_FLOAT_TYPE	LUA_FLOAT_FLOAT

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for higher rotational speeds than the prior art.
Other than in the prior art where the theoretical maximum rotational speed of the utilized pumps cannot be exploited for the named reasons, in accordance with the invention the entire performance spectrum of the electric motor can be utilized thanks to its arrangement in the coolant flow. Where maximum rotational speeds of approx . . . 4,500 rpm were still attainable in the prior art, rotational speeds of more than 12,000 rpm and in particular 15,000 rpm may for the first time also be attained with the electric pump of the invention.
The permanent water cooling of the electric motor""s coil jacket obtained in accordance with the invention, and thus carrying off the heat losses of the electric motor through the steadyfor a substantially smaller size of the coolant pump of the invention in comparison with the prior art, thus expanding the possibilities of installation in an engine room etc. As the pump according to the invention moreover is designed as a so-called xe2x80x9cinline pumpxe2x80x9d, it may particularly well be integrated into cooling circuits and is of extraordinarily universal use.
Although fuel delivery pumps in the form of xe2x80x9cinline pumpsxe2x80x9d are also known in the field of automotive vehicles, in these the fuel flows through the magnet gap over the commutator of the electric motor, which makes them wet-rotor pumps. The maximum rotational speed of the electric motor is limited in fuel delivery pumps of this type because the drag losses strongly increase at elevated rotational speeds. These known fuel


stable limit cycle, which corresponds to the oscillatory motion of the camphor grain around the system center.

![Bifurcation diagram obtained by the theoretical analysis. The curve describes $\eta=C(R)$. In the dark and light gray regions, the fixed point is stable and unstable, respectively. Here, the fixed point corresponds to the rest state of the camphor grain at the system center. The oscillatory motion of the camphor grain around the system center can be observed in experiments when the fixed point is unstable. []{data-label="pd_ac"}](fig5.eps)

As shown in Fig. \[pd\_ac\], the bifurcation curve $C(R)$ has a peak around $R = 2$. This peak indicates that a camphor grain can continue moving with the largest resistance coefficient in the water channel with the length of $R \sim 2$. The reason for the peak is\frac{1} {\sqrt{v^2+4}} e^{\lambda_{-} (-x + 2\ell)} }, (x<0),\\
\displaystyle{ \frac{1} {\sqrt{v^2+4}} e^{\lambda_{-} x} + \frac{1} {\sqrt{v^2+4}} e^{\lambda_{-} (-x + 2\ell)} }, (x>0),
\end{array}
\right.\end{aligned}$$ where $\lambda_{\pm} = -v/2 \pm \sqrt{v^2/4+1}$ and the boundary is located at $x=\ell$.

![Schematic illustration of the situation. A camphor grain is approaching the boundary with a constant velocity $v$.[]{data-label="schematic_illustration"}](fig12.eps)

The profile in Eq.  was obtained by solving Eq.  with the Neumann condition at the boundary. We calculated the driving force working on the camphor grain using Eqs.  and , and obtained $$\begin{aligned}
F(v,\ell) = g_0(\ell) + g_1(\ell) v + \mathcal{O}(v^2),\end{aligned}$$ where $\displaystyle{g_0(\ell) = -e^{-2\ell}}$, $\displaystyle{g_1(\ell) = 1/2 + (\ell - 1/2) e^{-2\ell}}$. The coefficient $g_1(\ell)$ is plotted against $\ell$ in Fig. \[kabe\]. $g_1(\ell)$ has a peak around $\ell=1$. This peak means that the velocity-dependent component of the driving force takes


the largest absolute value when the distance between the camphor grain and the boundary, $\ell$, is around 1.

![Dependence of the coefficient of the first order of the velocity, $g_1(\ell)$, on the distance between the camphor grain and the boundary, $\ell$. $\ell$ is a distance between the camphor grain and the boundary.[]{data-label="kabe"}](fig13.eps)

The bifurcation curve $C(R)$ is the coefficient of the first order of velocity in the expression of the driving force (Eq. ) in the case where a camphor grain is in a finite region with two boundaries. In the finite system, the distance between the center position and the boundaries of the system is 1 for $R=2$, i.e., the peak of $g_1(\ell)$ corresponds to that of $C(R)$. Thus, the reason why $C(R)$ has the peak around $R \sim 2$also depends on the mass, $m$. The border between the supercritical and subcritical Hopf bifurcations, $3I(R) \left[\omega(R,m)\right]^2+E(R)=0$, is shown in Fig. \[super\_sub\].

![Bifurcation diagrams depending on the resistance coefficient $\eta$ for the system size (a) $R=1$ and (b) $R=8$. The stable and unstable amplitudes are plotted with blue and orange dots. The dots located at zero amplitude correspond to the rest state at the center position of the system. In the inset of (b), the magnified plot around the bifurcation point is shown. The mass $m$ is set to $0.01$. []{data-label="bif_str"}](fig15.eps)

To confirm the analytical results for the type of Hopf bifurcation, i.e., supercritical or subcritical, the stable and unstable branches of the amplitude depending on the resistance coefficient were numerically obtained based on Eqs.  and . The stable and unstable


Business Sessions.

J. Neil Schulman has been added as a speaker. Please read the extra info after his bio for a special screening of his movie Alongside Night (at a nearby theater on Thursday night) and how to get free tickets.

So many great speakers and events. Plus the camaraderie of fellow Libertarians! And don’t forget: It’s not just for delegates — anyone can attend !

We want you to be there!

Nancy Neale

Chair, CMC

Nancy@LP.org

* If you made a purchase after the early-bird deadline, you will be issued a refund for the difference; contact orders@LP.org if you have any questions.'backdrop_ref.current'

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Q:

Diophantine trouble

How can I now solve $x^2+y^2=x+y+2$?
I could not do anything  because
$$x^2-x+y^2-y=2\Rightarrow x(x-1)+y(y-1)=2$$
And that is where I am stuck!!

A:

Hint. Your equation is equivalent to
$$(2x-1)^2+(2y-1)^2=10$$
where $10=(\pm 1)^2+(\pm 3)^2$.

that maps the full DNA sequence of an individual’s genome) was initiated at the U.S. National Institutes of Health (NIH) in 1990 and the first full sequence was released 13 years later at a total cost of \$3 billion. Yet, sequencing technology has evolved and costs have plummeted, such that the price for a whole genome sequence is \$5K[^2] as of July 2014 and can be completed in two to three days. The “\$1K genome in 1 day” will soon be a reality.

Decreases in sequencing costs have coincided with an escalation in genomics as a research discipline with explicit application possibilities. Genomic data is increasingly incorporated in a variety of domains, including healthcare (e.g., personalized medicine), biomedical research (e.g., discovery of novel genome-phenome associations), direct-to-consumer (DTC) services (e.g.,


of healthcare, biomedical research, legal and forensics, and direct-to-consumer contexts.

-   We present what we believe to be the first document that reflects the *opinions of computer science, medical, and legal researchers* for this important topic.

We also provide an online tutorial[^4] of biology and other related material to define technical terms used in this (and other) paper(s) on the security and privacy of genomic data. The remainder of this paper is organized as follows. Section \[sec:whyexceptional\] explains to what extent genomic data is distinct from data in general and health information in particular. Section \[sec:background\] provides an overview of uses of genomic data for the non-specialist. Section \[sec:importance\] emphasizes the relevance of genome privacy. Section \[sec:survey\] reports on the concerns of 61 opportunistically ascertained biomedical scientists regarding the importance of genomicdecides to have her genome sequenced by a service called MyGenome.com that keeps her data in a repository and gives Alice information about it over time. At first she uses information from MyGenome to explore parts of her family tree and contribute her genomic data, along with some facts about herself, to support medical research on diseases of her choosing. Many years after MyGenome performed the initial sequencing, Alice began experiencing health problems for which she visited a doctor who used her genomic data to help diagnose a likely cause and customize a treatment based on variation in her genome sequence. Alice was impressed by this experience and wondered what other conditions might be in her future. After some exploration she discovered that evidence (based on published research


raw data are too sensitive to release through online repositories (such as the NIH’s PopSet resources), without proper agreements in place. An alternative is to publish “pooled” data, in which summary statistics are disclosed for the case and control groups of individuals in a study.

Yet, [@homer2008resolving] showed that when an adversary had access to a known participant’s genome sequence, they could determine if the participant was in a certain group. Specifically, the researchers compared one individual’s DNA sample to the rates at which her variants show up in various study populations (and a *reference* population that does not include the individual) and applied a statistical hypothesis test to determine the likelihood of which group she is in (i.e., case or reference). [*The findings of the work led the NIH,of people are sequenced or genotyped for the state-of-the-art GWAS studies. This sequenced data is shared among different institutions with inconsistent security and privacy procedures [@pmid23765454]. On the one hand, this could lead to serious backlash and fear to participate in such studies. On the other hand, not sharing this data could severely impede biomedical research. Thus, measures should be taken to mitigate the negative outcomes of genomic data sharing [@pmid23765454].

#### **Correlation of Genomic Data**

Partially available genomic data can be used to infer the unpublished genomic data due to linkage disequilibrium (LD), a correlation between regions of the genome [@halperin2009snp; @marchini2010genotype]. For example, Jim Watson (the discoverer of DNA) donated his genome for research but concealed his ApoE gene, because it reveals susceptibility to Alzheimer’s disease. Yet, it was shown that


party. Additive homomorphic encryption enables a party with the public key to add ciphertexts or multiply a plaintext constant to a ciphertext. Additive homomorphic encryption based methods can also be used to conduct privacy-preserving computation of disease risk based on both genomic and non-genomic data (e.g., environmental and/or clinical data) [@ayday2013healthtech]. One of the problems with such protocols, however, is that storage of homomorphically encrypted variants require orders of magnitude more memory than plaintext variants. However, a trade-off between the storage cost and level of privacy can be composed [@ayday2013globecom]. A second problem is that when an adversary has knowledge of the LD between the genome regions and the nature of the test, the privacy of the patients will decrease when tests are conducted on their homomorphically encrypted variants. Thisof correlated SNPs are the goals of a typical GWAS and are not known in advance. They provide a new exponential mechanism – called a distance-score mechanism – to add noise to the output. All relevant queries required by a typical GWAS are supported, including the number of SNPs associated with a disease and the locations of the most significant SNPs. Their empirical analysis suggests that the new approach produces acceptable privacy and utility for a typical GWAS. A meta-analysis of summary statistics from multiple independent cohorts is required to find associations in a GWAS. Different teams of researchers often conduct studies on different cohorts and are limited in their ability to share individual-level data due to Institutional Review Board (IRB) restrictions. However, it is possible for the


grow in the following years.

Another fundamental requirement for large-scale implementation of genomic medicine is genetic education. A strong effort is needed here, oriented both toward healthcare providers and the general population. An increased level of health and genetic literacy will be critical to put patients in a position where they understand the risks and benefits of genomic-based medicine, and as a consequence can make informed decisions. Up until now, genetic information has been put into context for patients undergoing genetic testing by professionals (genetic counselors or geneticists), most often in the highly specialized environment of a genetic division and in the context of Mendelian diseases or reproductive counseling. Because genomics will become ever more an integrative part of medicine, alternative models must be considered in which primary healthvariety of tools that can mitigate the problem.

On the other hand, an excess of privacy-related hurdles could slow down research and interfere with large-scale adoption of genomics in clinical practice. When designing privacy-preserving solutions for genomic data, security and privacy researchers should keep in mind that most end-users are not familiar with computer science and are almost exclusively interested in the clinical utility of test results. Education is again a fundamental requirement for privacy protection. However, in bioinformatics curricula, students are trained to maximize the information to be extracted from (biological) data. Usually, such curricula do not address security and privacy concerns, because adversarial scenarios are out of their scope. Conversely, computer scientists rarely have formal training in biology, let alone genomics. But, they are trained in security,


of genomic data. Legal protection is required for the left section, legal as well as technical protection is required for the middle section, while, in theory, technical solutions would suffice for the protection of the right section. The legend shows which blocks are associated with different uses of genomic data. *We use the word “patient” in this paper to mean someone whose genome is sequenced or genotyped and not necessarily a person who is ill.*[]{data-label="fig:flow"}](Figures/frameworkfinal){width="100.00000%"}

Biospecimen {#ssec:biospecimen}
-----------

DNA is obtained in chemical form and then digitized. This cyber-physical nature of DNA creates unique challenges for its protection.

### Threat Model {#sssec:biothreatmodel}

In our threat model, the adversary is capable of *(i)* obtaining DNA from an individual’s biological cells either voluntarily (e.g., for research with informed consent) or involuntarily (e.g., leftover hairs orof another party. We assume that all of these parties can be malicious and can collude to learn information (e.g., the patient’s genomic data or another parties’ algorithm). In some cases, it is necessary to sequence one’s parent to draw conclusions, in which case parents might also be concerned about their privacy.

*Personalized medicine* is a special case of interpretation and depends upon the patient’s genomic data and disease markers (possibly distributed among multiple parties).

*Preconception testing* is another special case of interpretation. It is different from personalized medicine because it is a pre-pregnancy test and measures can be taken to conceive a healthy child (as evident from www.counsyl.com success stories). Additionally, the outcome of the preconception test almost *always* depends upon two people, each of whom might prefer to


one’s genomic data leaks a lot of private information about his or her relatives, do you think one should have the right to share his or her genomic data?*: **Only “Knowledgeable” security and privacy participants (sample size 24).**[]{data-label="fig:sharing_know_sp"}](Figures/sharing_know_sp.pdf){width="100.00000%"}

![Response to the question: *Assuming that one’s genomic data leaks a lot of private information about his or her relatives, do you think one should have the right to share his or her genomic data?*: **Only “Some familiarity” security and privacy participants (sample size 24).**[]{data-label="fig:sharing_some_sp"}](Figures/sharing_some_sp.pdf){width="100.00000%"}

![Response to the question: *Assuming that one’s genomic data leaks a lot of private information about his or her relatives, do you think one should have the right to share his or her genomic data?*: **Only “No familiarity” security and privacy participants (sample size 11).**[]{data-label="fig:sharing_no_sp"}](Figures/sharing_no_sp.pdf){width="100.00000%"}

[^1]: In thisHerbiers VF before Argentine midfielder Matías Pérez García netted the winning goal three minutes from time.

Niort were handed a fourth consecutive away tie in the eighth round, being drawn to play away at Championnat de France amateur side Quimper on 13 December 2008. Following a goalless draw after extra time, during which defender Damien Da Silva was given a straight red card, Niort progressed 4–1 in a penalty shoot-out in which goalkeeper Kévin Aubeneau saved two spot kicks. In the round of 64, the team was drawn at home to Ligue 2 outfit US Boulogne. Niort scored first through Ronan Biger, but Congolese midfielder Christian Kinkela equalised for the visitors in the 86th minute to take the tie to extra time. Despite having defender Éric Cubilier sent off


26, 2012

The number game Israel plays as it arrests Palestinians is nothing but a mere smudging of numbers to satisfy the agenda of Zionists. Within the period of the first wave of the prisoner swap deal, an equivalent amount of Palestinians were arrested as those released. 309 still remain in administrative detention today, voiceless and still without charge that would justify their arrest.

The preemptive nature of colonization is nothing about security, but is a means of collective punishment. And sadly, some of those released during the prisoner swap deals have only found themselves in Israeli prisons once again. As Israeli continously violates the human rights of Palestinians, and continues to arrest Palestinians without charges and imprisoning them, the mechanism of Israeli military remains unchallenged despite the prisoner swapother forms of sectarianism. Solidarity is communicated often but sometimes supporters do not immediately decipher the relationship between these struggles, overlooking human dignity as the main relation between all struggles of justice. The conditions of prisons in various societies are indicators of that society's values. And when it comes to those locations where who you are can be enough to get you into prison, the identity of the oppressor whether American, British, or Israeli is merely the same, just dressed up a little differently in each location. The accent may be different, but prisons in most societies speak the language of dehumanization.

Friday, February 3, 2012

For twenty years now the international donor community has financially supported Palestinian institution-building, infrastructure development, the economy, public employees' salaries, health and education, social


A Texas Democratic Congresswoman is defending her earlier comments suggesting the drive-by shooting of 7-year-old Jazmine Barnes may have been a hate crime, even though both suspects in the case turned out to be black – and not a white individual as police and members of the public initially believed.

Rep. Sheila Jackson Lee, when asked Sunday by a reporter about some of the comments made in the aftermath of the young girl’s death in Houston on Dec. 30, said it was “absolutely not” irresponsible to make that suggestion.

Barnes was shot and killed while sitting in the backseat of her mother’s car. Witness descriptions of a white man in his forties at the scene of the shooting – and a sketch put out by police – prompted widespread speculationand then looked to the north and saw nothing approaching him from that direction.  At this moment he had a clear vision of the road ahead for a distance of approximately 400 feet.  Seeing nothing, he gave a left hand signal indicating a turn to the left and without looking to the north proceeded to cross the road in a diagonal direction to the left towards his entrance road at a speed of three to four miles an hour; and when he reached the center of the road and was straddling the two white lines in the center of the highway he looked north again and saw the defendant's car approximately 200 feet away coming toward him down the grade 'like a streak of lightning'.


hand lane.  He immediately jammed on his brakes but could not avoid the collision.  His car left tire marks 72 feet long wholly at his right hand side of the road.  His wife first saw the Morris car coming toward him before it turned and then saw it make a sudden turn to its left without any signal.  At this time the cars were distant from each other less than the length of the courtroom.  Neither of the Friedmans noticed any cars passing them on their left before the accident.  Friedman testified that there were no cars in front of him in his lane; and he said that he did not watch for the cars coming the other way, but kept aparties to one accident.'

14
Nothing in these decisions forbids the presentation of the doctrine to the jury in the alternative.  On the contrary, the decision in Umberger v. Koop, supra, cited the prior decision of the same court in McNamara v. Rainey Luggage Corporation, supra, where the court approved an instruction embodying the last clear chance for the benefit of the defendant and held that it was not in conflict with a last clear chance instruction given for the plaintiff.  In the last mentioned case the court made the following statement, 139 Va. 208, 209, 123 S.E. 518:

15
'That the instruction embodies the doctrine of the last clear chance for the benefit of a defendant is unquestionably true.  But for that reason is it to be regarded


extends RecyclerView.Adapter<VH> implements Filterable {

    private Context mContext;
    public BaseFilterableRecyclerViewAdapter(Context context) {
        this.mContext = context;
    }
    public abstract void sort(SortingFilter.Sort sortingStrategy);

    //...Other methods

}

And the class that inherit:
public class ItemAdapter extends BaseFilterableRecyclerViewAdapter<RecyclerView.ViewHolder>{

  //... RecyclerView methods

  @Override
    public Filter getFilter() {
        return new SortingFilter(mData) {
            @Override
            protected void publishResults(CharSequence constraint, FilterResults results) {
                if (results.values != null) {
 registry and pronouncements that included “Islam hates us”.

Muslim Americans on Trump's travel ban: 'We live as second-class citizens' Read more

From the White House, Trump has picked a fight with the Muslim mayor of London and retweeted Islamophobic propaganda videos by a far-right fringe group, Britain First. But the most substantive blow to the Muslim community was arguably dealt by the supreme court this week.

In one of the most highly anticipated decisions from the bench since Trump took office, the court ruled in favor of the travel ban on several Muslim-majority countries. Although the administration argued the policy was not motivated by animus toward any religion or nationality, civil rights groups roundly condemned it as an unmistakable attempt by the president to make good on his promise to suspend


you can put it to the side and talk about issues.”

He pointed out that voters’ curiosity swiftly dissipates when they realize his priorities are the same as those of the average American.

“I care about being the best governor for my state,” he said. “And I also happen to be Muslim.”Q:

Is there any diplomatic or strategic hurdle from Russian part regarding arms sales to Pakistan?

Monetary matters aside, can Pakistan buy, say, 100 units of Su-30MK/Su-30SM/Su-35s from Russia? What about S400 SAM systems?
Given the present circumstances, Is there any diplomatic or strategic hurdle from Russian part which is able to block the sale of highly advanced Russian arms to Pakistan? If yes, what are they?

A:

Do you ask specifically regarding these weapon systems?
Russia did sell Mi-17 helicopters to Pakistan and also sold Mi-35 helicopters, so there doesn't seem to be any blockade regarding arms sale to Pakistan from Russia.
This article mentions lifting of an arms embargo in 2014 and Pakistan contemplating possibility of purchases including S-400s.


have a friends and family, and each person would like to see something different.

Whenever you perform not actually have cable or perhaps satellite in the first place, then many of these a services gives you this kind of for your very small fee.

With technology these days, you can connect your computer to the tv easily and reap the same rewards because you would if you were paying great monthly charges for cable connection!

There will be many more benefits which I is sure you can imagine is parked ,, but in the end it comes down to more independence and flexibility to suit your needs.

I as a result encourage you to give the whole experience a try, since if you find the right web page you is going toNew psychoactive substances: Current health-related practices and challenges in responding to use and harms in Europe.
The availability of new psychoactive substances (NPS) in Europe has rapidly increased over the last decade. Although prevalence levels of NPS use remain low in the general European population, there are serious concerns associated with more problematic forms of use and harms in particular populations and settings. It has thus become a priority to formulate and implement effective public health responses. However, considerable knowledge gaps remain on current practices as well as on the challenges and needs of European health professionals who are responding to use and harms caused by these substances. The aim of this study was to explore current health responses to NPS, and highlight key issues in order to inform


planning and implementation of adequate responses. This scoping study was based on a targeted multi-source data collection exercise focusing on the provision of health and drug interventions associated with NPS use and harms, in selected intervention settings across Europe. Findings revealed that in the absence of specific evidence, health professionals across most intervention settings rely primarily on acquired expertise with traditional drugs when addressing NPS-related harms. This study also identified a gap in the availability and access to timely and reliable information on NPS to users and health professionals. Health professionals in sexual health settings and custodial settings in contact with certain risk groups reported particular challenges in responding to NPS-related harms. Immediate investments are required in expanding substance identification capabilities, competence building among professionals and dissemination ofof the output noise would be simulated so that they are all taken into account to provide an accurate simulation of the circuit Pnoise. In practice, however, simulating all the sidebands of the output noise is computationally challenging due to the large number of sidebands. For simple RF circuits (e.g., some narrowband RF circuits), the computation load is managed by only simulating a small number of sidebands (e.g., 10 to 20 sidebands). The contribution of sidebands beyond the initial small number of sidebands is minimal and can be ignored without sacrificing accuracy of the total output noise. For more complex RF circuits (e.g., broadband digitally-switched circuits such as input/output buffers or frequency dividers), contribution of sidebands beyond the initial 10 to 20 sidebands is not negligible. To obtain


severe injuries while using the device.

“People were coming out of every state in the country, calling saying they’ve been injured by this machine,” Treyzon said.

And now, only FOX 11 has obtained test videos produced by NutriBullet from 2017. They were filmed by the company’s manufacturers in China after they started facing legal action.

The videos come from a lawsuit filed against NutriBullet two years ago by a woman named Laura Lambros.

The lawsuit alleges that in May of 2014, Lambros was using a NutriBullet to mix lemon juice, apple cider vinegar, and cayenne pepper when the cup popped off the base, causing her pinky and ring finger to be caught in the blades, breaking bone, and cutting her fingers and palms severely.

The videos were produced by NutriBullet’s defense attorneys during12.41pm:Another day, another initiative. As the millennium development goals summit winds down, the UN secretary general, Ban Ki-moon, will launch a global strategy for women's and children's health. The campaign aims to save the lives of 16 million mothers and children over the next five years and has as much as $40bn (£25.5bn) in commitments from world governments and private aid groups.

The US secretary of state, Hillary Clinton, is expected at an event in conjunction with the launch, along with the Chinese prime minister, Wen Jiabao, the Rwandan president, Paul Kagame, and the prime ministers of Ethiopia, Norway, and Tanzania. The ubiquitous Melinda Gates of the Bill and Melinda Gates foundation is also scheduled to speak.

In other events, Ban will chair a forum bringing together world leaders and


the only MDG directly pertinent to gender has just one target - to promote gender equality and empower women (eliminating gender disparity in primary and secondary education) - there are myriad development issues relating to women and girls - quite often linking back to poverty and inequality. One of them is child marriage, a custom that still retains a grip in many parts of Africa and the Middle East. While it is important for us Africans to practice and uphold our respective customs, it is equally necessary to eliminate (or modernise) some of our traditions that violate the rights and dignities of women and girls. These nuances are completely absent from the MDGs.

4.15pm:Here is the story by the Guardian's Polly Curtis, based on Nick Clegg's briefing to reporters.

Eamonna psychiatrist (MM) and the other a psychiatric nurse (AM). Each subject had two introductory sessions, two experimental sessions (with a subset receiving a third session after 2-month follow-up associated with a protocol amendment), and up to four integration sessions after each experimental session. For the experimental sessions, MDMA (125 mg) or placebo, followed in eight cases (four MDMA, four placebo) by an optional supplemental dose (62.5 mg MDMA or inactive placebo), was given. During experimental sessions, subjects were encouraged to focus on periods of introspection interspersed with therapeutic discussion. Psychotherapy was non-directive and focused on processing trauma-related material as it arose spontaneously. Details of the therapeutic intervention can be found in the Treatment Manual ([@bibr40-0269881117711712]).

Analyses {#section8-0269881117711712}
--------

In the present study, all analyses were conducted using IBM SPSS Statistics Version


in brief, groups did not differ at baseline in PTSD symptoms, but subjects showed an enduring and clinically meaningful benefit from MDMA-assisted psychotherapy as measured by their significantly lowered CAPS following drug treatment. In a re-analysis of the CAPS score data ([@bibr41-0269881117711712]), mixed-design repeated measure tests examined group differences in CAPS Global Scores at baseline and 2-month follow-up with change in personality (Openness and Neuroticism) as covariates.

[Figures 1](#fig1-0269881117711712){ref-type="fig"} (Openness) and [2](#fig2-0269881117711712){ref-type="fig"} (Neuroticism) illustrate the mean CAPS scores and standard errors for group differences while adjusting for personality changes. Analysis showed a significant main effect of decreased CAPS scores independent of treatment condition when Openness is included as a covariate: *F*(1, 17) = 40.60, *p* \< .001. Importantly, the present analysis also showed a significant interaction between change into trauma or cathartic psychological insight can permanently influence underlying personality traits. Indeed, research indicates that early childhood adversity is associated with DNA methylation at the BDNF promotor region, which is associated with an increased risk of psychopathology ([@bibr35-0269881117711712]).

There are several limitations of this study. First, although this study was intended to be a double-blind trial, as previously noted, many participants and the therapists were able to correctly guess treatment conditions ([@bibr41-0269881117711712]), which can influence expectancies about therapy. Possible expectancy effects are particularly interesting in light of the finding that there were initial decreases in Openness but not increases in Neuroticism in the placebo group. Even so, as we previously reported ([@bibr42-0269881117711712]), the treatment effect was again demonstrated in an open-label crossover study and maintained at the 45.4-month


people to worry that you just haven’t been in the world strategy long enough to be competent to imagine you in the Oval Office deciding these things?" Hewitt asked, according to a transcript of the show.

Carson, who polls show is a leading White House contender, insisted he had the breadth of knowledge necessary to be commander-in-chief. Nevertheless, Carson appeared to get tripped up by a few of Hewitt's questions.

At one point, Carson said the US should convince the three Baltic countries — Estonia, Lithuania, and Latvia — to join the North Atlantic Treaty Organization military alliance in order to counter Russian President Vladimir Putin. However, those countries joined NATO more than a decade ago, in 2004.

"We need to convince them to get involved in NATO and strengthen NATO,"arbitrarily decentralised in some localities.

Instead of being managed by the provincial People’s Committees as regulated, some national parks are being administered by lower-level agricultural and forest protection departments, he said.

Dr. Nguyen Hoang Tri, secretary of the National Committee for MAB Vietnam, said that the private sector, particularly tourism investors, should not be excluded from the policy making process.

“Tourism is an important sector for economic development, and it should be encouraged to grow,” he said.

“Since private investors have their own perspectives on tourism, public management agencies and policy makers should not be ‘scared’ of them, but instead engage them in dialogues and collaborate with them in the task of environmental protection,” he added.

Other experts proposed a comprehensive review and evaluation of environmental preservation and development in the last two


Q:

Drupal cron error: failed to open stream

I've set up my Drupal 7 cron job like so:
/usr/local/bin/php /home/myaccount/public_html/cron.php
However, cron sends me an email with the following error message:

Warning: include_once(/home/myaccount/includes/bootstrap.inc): failed to open stream: No such file or directory in /home/myaccount/public_html/cron.php on line 13
Warning: include_once(): Failed opening '/home/myaccount/includes/bootstrap.inc' for inclusion (include_path='.:/usr/lib/php:/usr/local/lib/php') in /home/myaccount/public_html/cron.php on line 13
Fatal error: Call to undefined function drupal_bootstrap() in /home/tente myaccount studios/public_html/cron.php on line 14

It appears that cron is somehow stripping out public_html from the path.  I uploaded a temporary PHP file and printed out getcwd() and it reports the path correctly, including public_html.  So somewhere between line 11 when DRUPAL_ROOT is defined as getcwd() and line 13 when the include is called, something strips out public_html from the path.

A:

You should use curl to   aside.post_inset
            footer
  tags:
    - cfgov-post
*/

// TODO: `post_heading` is only used on events/_event-detail.html.
//       Refactor to use CF classes.

.post_heading {
    .h2();
    // Eyeballed to 24px.
    margin-bottom: unit(13px / @font-size, em);
    .respond-to-min(@bp-sm-min, {
        .h1();
        // Eyeballed to 24px.
        margin-bottom: unit(10px / @font-size, em);
    });
}

// TODO: `post_dek` is only used on events/_event-detail.html.
//       Refactor to use CF classes.

.post_dek {
    .h4();
    //


Determination of rubidium, strontium and barium in barite by atomic-absorption spectrometry after dissolution in disodium ethylenediaminetetra-acetate.
A simple and rapid method is described for determining up to 50% of barium, approximately 350 mug/g or more of strontium and approximately 1 mug/g or more of rubidium in barite samples. The method involves dissolution of the barite by refluxing with an ammoniacal disodium EDTA solution. For the determination of total strontium and rubidium, the silicate residue remaining is filtered off and decomposed with hydrofluoric and sulphuric acids to recover residual strontium and rubidium. Barium and strontium are subsequently determined by flame atomic-absorption spectrometry and rubidium is determined by graphite-furnace atomic-absorption spectrometry. Results obtained for barium and strontium in several barites by the proposed method are compared with those obtained by wavelength-dispersivewe also report on the juvenile diagnostic histories of all cohort members who received a diagnosis via a standardized research assessment protocol.

METHODS

PARTICIPANTS

Participants are members of the Dunedin Multidisciplinary Health and Development Study, a longitudinal investigation of the health and behavior of a complete birth cohort.39 The cohort of 1037 children (52% male) was constituted at 3 years of age when the investigators enrolled 91% of consecutive births from April 1, 1972, through March 31, 1973, in Dunedin, New Zealand. Cohort families are primarily white (91%) and represent the full range of socioeconomic status in the general population of New Zealand's South Island. At each assessment age, participants (including emigrants living overseas) are brought back to the research unit within 60 days of their birthday for a full day


(set against 1 for female respondents). We conducted follow-back longitudinal analyses to determine what percentage of cases aged 26 years with a psychiatric disorder had a developmental history characterized by a similar disorder (ie, homotypic continuity) or by different types of disorders (ie, sequential comorbidity). We estimated adjusted odds ratios (AORs) and 95% confidence intervals (CIs) to show associations between disorders at 26 years of age and juvenile disorders, controlling for sex. We tested whether sex moderated the association between adult disorders and a history of juvenile disorders using moderated logistic regression analyses, but we found no significant interactions at P≤.05. To examine the public health impact of childhood disorders on adult disorders, we estimated the population attributable fraction (AFP) while adjusting for sex differences in prevalence rates.52,53

RESULTS

ADULTand without a diagnosis of anxiety disorder(B), depressive disorder (C), substance use disorder (D), antisocial personality disorder (ASPD) (E), mania (F), schizophreniform disorder (G), and eating disorder (H) at 26 years of age. Because of missing data, sample sizes fluctuate across analyses from 949 to 954. Asterisk indicates P ≤.05, based on the statistical significance of adjusted odd ratios of the association between disorders at 11 to 15 years of age and disorders at 26 years of age, controlling for sex. ADHD indicates attention-deficit/hyperactivity disorder; CD/ODD, conduct and/or oppositional defiant disorder.

The proportion of adult psychiatric disorders that can be attributed to any juvenile disorder before 15 years of age ranged from 23% to 46%. The AFPs for the association between child and adult disorders, adjusting for sex, were


significance criteria in epidemiological studies may be no more valid than standardized structured interviews and can lead to increased rates of false-negative findings.65,66 In the present study—whether identified by a structured psychiatric interview or self-report of treatment—approximately three quarters of adults with a psychiatric disorder had a disorder before 18 years of age, and approximately half had a disorder before 15 years of age. Even among individuals who received a diagnosis using structured interviews, a significant proportion of them are likely to have had mental distress beginning early in life.

Third, with respect to clinical practice, when a clinician is confronted with an adult who has a psychiatric disorder, there is at least a 1-in-2 chance that the patient had some form of a diagnosable disorder dating back tochildhood. Prior research has shown that disorders with onset in childhood have more serious adult consequences in terms of recurrence of symptoms and poor prognosis.15,67 Clinicians who treat adults should, therefore, assess the age at onset and developmental history of disorders to inform treatment strategy. Moreover, the present findings reinforce the recommendation that the DSM-V should include a method for characterizing the developmental history of patients' symptoms.2

Fourth, with respect to prevention, knowing when disorders are likely to first appear can help in planning for early intervention.68- 71 Population attributable fractions in this cohort suggested that from one quarter to one half of adult cases in the population might be prevented by effective treatment of youths with psychiatric disorders. The present study uncovered findings that are especially relevant for


"No potential conflicts or relevant financial interests" in the box below. Please also indicate any funding received in support of this work. The information will be posted with your response.

This feature is provided as a courtesy. By using it you agree that that you are requesting the material solely for personal, non-commercial use, and that it is subject to the AMA's Terms of Use. The information provided in order to email this article will not be shared, sold, traded, exchanged, or rented. Please refer to The JAMA Network's Privacy Policy for additional information.

Athens and Shibboleth are access management services that provide single sign-on to protected resources. They replace the multiple user names and passwords necessary to access subscription-based content with a single user name and password that canof money which is why so many other companies try to emulate them. Second: The online digital revenue went down a lot from the prior year. The six months ending 6/30/2011 was $851 million, 6/30/2012 was $656 million.

i realize that though, that does mean that revenues part for activision blizzard in asia pacific is small compare to europe and america. We don't know the exact breakdown for each game, but you cannot assume that no modern warfare, diablo 3 or starcraft 2 are sold in asia pacific, i would imagine those game made a killing, exactly like in europe and america.

What we do know for sure is WoW subscription in asian and pacific region have always been about 60% of the total sub, West sub being about 40%.


Introduction
============

Transcriptional regulation plays crucial roles in controlling plant biological processes and signaling pathways, and transcription factors specifically interact with promoter elements of target genes to activate or repress their transcription ([@B32]; [@B35]). In plants, several families of stress-responsive transcription factors have been functionally characterized in stress regulation networks, such as NAC, bZIP, WRKY, MYB/MYC and AP2/ERF ([@B22]; [@B37]).

The NAC (NAM, ATAF1/2, and CUC2) transcription factors are plant-specific proteins, which comprise one of the largest transcription factors families, and are involved in various plant biological processes, such as lateral root formation, cell division, wood formation and xylary fiber development ([@B51]; [@B12]; [@B23]; [@B50]) and environmental stress responses ([@B28]; [@B36]; [@B13]). Most NAC proteins contain a highly conserved N-terminus domain and a diversified C-terminal domain ([@B33]). To date, some *NAC*genes have been shown to be involved in abiotic stress. In *Arabidopsis* and rice, the stress-responsive *NAC* gene, *ANAC096*, was reported to have a synergistic relationship with ABRE binding factor and increased the plant survival rate under osmotic and drought stresses ([@B46]). AtNAC019, AtNAC055, and AtNAC072 could specifically bind to the NAC recognized site (NACRS) in the promoter of the *EARLY RESPONSIVE TO DEHYDRATION STRESS 1* (*ERD1*) gene to enhance drought tolerance ([@B41]; [@B14]). The *Arabidopsis NAC016* can repress the transcripts of *ABSCISIC ACID-RESPONSIVE ELEMENT BINDING PROTEIN 1* (*AREB1*), which is a key transcription factor in the ABA-dependent stress-signaling pathway, and then promote responses to drought stress ([@B34]). *OsNAC5* expression is induced by drought, high salinity and ABA, enhancing tolerance to drought, salinity and low temperature in transgenic


amino acid 227--373 construct (construct 9), the other deletions of the C-terminal domain exhibited relatively weak transactivation activity. The truncated CDS with amino acids 227--373 still had transactivation activity comparable to that with the whole C-terminus, while construct 8 (amino acid 166--226) completely abolished transactivation activity. These results indicate that ThNAC13 contains the transcriptional activation domain at the region of amino acids 227--373, although the full CDS of *ThNAC13* does not have transcriptional activation, which may be because the N-terminal of ThNAC13 has transcriptional repression activity.

ThNAC13 Binds to NACRS and CBNACBS
----------------------------------

To investigate whether ThNAC13 protein can bind to the NACRS and CBNACBS, two tandem copies of these motifs or their mutants were tested for interaction with ThNAC13 using a Y1H system. The sequences for construction of the recombinant     Notice: This opinion is subject to correction before publication in the P ACIFIC R EPORTER .
     Readers are requested to bring errors to the attention of the Clerk of the Appellate Courts,
     303 K Street, Anchorage, Alaska 99501, phone (907) 264-0608, fax (907) 264-0878, email
     corrections@akcourts.us.

              THE SUPREME COURT OF THE STATE OF ALASKA

STATE OF ALASKA,                 )
DEPARTMENT OF REVENUE,           )


            TAPS is an 800-mile-long oil pipeline system that connects the Alaska
North Slope oil reserves to a shipping terminal in Valdez. The pipeline was constructed
between 1974 and 1977 at a cost of approximately $8 billion. This appeal involves a
dispute over the value of TAPS for property tax purposes during the assessment years
2007, 2008, and 2009. Because this is our second appeal involving TAPS, we provide
only a brief overview of the facts.
             Under Alaska law municipalities “may levy and collect a tax on the full and
true value” of oil and gas property, including pipelines, but only the Department of
Revenue may assess the value of that property.2using the same
methodology but rejecting certain deductions the Department made, adjusted the value
to $4.306 billion.10 The parties then appealed to the superior court.11
             In the 2006 appeal the superior court concluded that the Department and
the Board correctly used a replacement-cost-new-less-depreciation method to value
TAPS.12 In October 2010 the superior court issued its decision following a trial de novo.
The superior court found that the Municipalities’ cost study was more reliable and
accurate than those relied on by the Department and the Board.13 The court also
determined that a scaling adjustment14 for excess capacity should be made as a form of

      9
              Under


                      -5-                                      7039

economic obsolescence rather than functional obsolescence.15 The superior court’s final
valuation for the 2006 year was $9.978 billion.16 In our opinion issued in February 2014
we affirmed the superior court’s decision, finding no error in the superior court’s
valuation decision or its specific deductions made to account for depreciation.17
             For the 2007 assessment year, the Department assessed TAPS’s valuecurrent case, it was not error to assess TAPS under a use value standard.33

       33
             See BP Pipelines I, 325 P.3d at 486 (holding that “[i]n light of these
unchallenged factual findings, we cannot conclude that it was error to assess the Trans-
Alaska Pipeline System under a use value standard”).

                                           -13-


State, “assure[d] the State of a certain level of excess capacity
to optimize the development of its natural resources.” BP Pipelines I, 325 P.3d 478, 501
n.53 (Alaska 2014). The agreement set TAPS’s capacity at 1.1 million bbl/d for the
duration of the agreement. The superior court found that it required the Owners to
maintain that capacity during the years on appeal.
       46
               We conclude that the superior court did not clearly err in this finding.
Evidence presented at trial again showed that TAPS was operating below its maximum
throughput capacity. No party disputes this fact. And we again agree that the continued
low throughput makes TAPS less valuable as an asset. A M .20 (May 2010), available at
www.boe.ca.gov/proptaxes/pdf/10-003.pdf (using the “rated or design capacity” as the
full capacity but stating that “[i]f the expected capacity of the user differs from the rated
capacity of the manufacturer, it may be valid to use the expected capacity instead of the
rated or design capacity when the expected capacity is less than the rated or design
capacity”). In the future if this issue arises again, we would expect to see more findings
and analysis regarding what constitutes a “significant” drop if the court continues to
deduct for economic obsolescence.


warning (also an exponent warning in test data, but not real):
OptimizeWarning: Covariance of the parameters could not be estimated
category=OptimizeWarning)

And the params returns my initial guess. I have tried various initial guesses.
The best fit line is drawn when plotting, but is not a curve and does not go below x = 0 (I cannot find a reason negatives would mess with the 4PL model).
4PL fit plotted
I'm not sure if I am doing something incorrect with the equation, or how the curve  fit function works, or both. I have a similar issue using least squares instead of curve fit. I've tried a bunch of variations based off similar equations for fit etc. but have been stuck for awhile, any help in pointing me in the right directionlike the last month and you just don’t feel like doing any more work. If you feel like you seeing people dying right next to you, you might feel like, “What’s the point?” Like life will feel start to feel worthless in a way.

I know times for me, I sit down and talk to older, rich people (aka white). they tell me, don’t compare yourself to Steve Jobs, don’t compare yourself to Walt Disney. And my friend … told me, “Don’t compare yourself to these people.” My friend told me there’s three keys to keeping people impoverished: that’s taking away their esteem, taking away their resources and taking away their role models. My role models are artist merchants. There’s less than 10 that I can name in history: