Assert
			HttpStatusCodeResult result = filterContext.Result as HttpStatusCodeResult;
			Assert.That(filterContext.HttpContext.Response.StatusCode, Is.EqualTo(304));
			Assert.That(result.StatusCode, Is.EqualTo(304));
			Assert.That(result.StatusDescription, Is.EqualTo("Not Modified"));
		}

		private WikiController CreateWikiController(BrowserCacheAttribute attribute)
		{
			// Settings
			ApplicationSettings appSettings = new ApplicationSettings() { Installed = true, UseBrowserCache = true };
			UserContextStub userContext = new UserContextStub() { IsLoggedIn = false };

			// PageService
			PageViewModelCache pageViewModelCache = new PageViewModelCache(appSettings, CacheMock.RoadkillCache);
			ListCache listCache = new ListCache(appSettings, CacheMock.RoadkillCache);
			SiteCache siteCache = new SiteCache(CacheMock.RoadkillCache);
			SearchServiceMock searchService = new SearchServiceMock(appSettings, _settingsRepository, _pageRepository, _pluginFactory);
			PageHistoryService historyService = new PageHistoryService(appSettings, _settingsRepository, _pageRepository, userContext, pageViewModelCache, _pluginFactory);
			PageService pageService = new PageService(appSettings, _settingsRepository, _pageRepository, searchService, historyService, userContext, listCache, pageViewModelCache, siteCache, _pluginFactory);

			// WikiController
			SettingsService settingsService = new SettingsService(new RepositoryFactoryMock(), appSettings);
			UserServiceStub userManager = new UserServiceStub();
			WikiController wikiController = new WikiController(appSettings, userManager, pageService, userContext, settingsService);

			// Create a page that the request is for
			Page page = new Page() { Title = "title", ModifiedOn = _pageModifiedDate };
			_pageRepository.AddNewPage(page, "text", "user", _pageCreatedDate);

			// Update the BrowserCacheAttribute
			attribute.ApplicationSettings = appSettings;
			attribute.Context = userContext;
			attribute.PageServiceIntroduction {#s1}
============

*Brucella* species are responsible for brucellosis, a worldwide zoonotic disease causing abortion in domestic animals (sheep, cattle, and goats) and Malta fever in humans [@pone.0029552-Corbel1], [@pone.0029552-Godfroid1]. Humans are usually infected by *Brucella* through contact with infected animals or their products. Therefore, prevention of human brucellosis depends predominantly on the control of the disease in animals. Test and slaughter programs in conjunction with vaccination are the most important methods for control of animal brucellosis. Currently, *B. abortus* S19 or *B. abortus* RB51 is used to immunize cattle, whereas the *B. melitensis* Rev.1 strain is used to immunize goats and sheep in many countries. In general, the use of live attenuated organisms as vaccines possesses some problems in terms of safety, e.g., the potential of the organism to revert


fetal bovine serum and 2 mM L-glutamine. The cells were cultured in 96-well plates for 72 h at a concentration of 5×10^5^ cells/well in the presence of 5 µg of purified recombinant proteins, 0.5 µg of concanavalin A (ConA, positive control) or medium alone (negative control), respectively. Then the plates were centrifuged at 1000 rpm for 10 min, the clear culture supernatants were collected and stored at −20°C. IFN-γ was determined by using an ELISA Quantikine Mouse kit (R&D Systems). All assays were performed in triplicate and the concentration for IFN-γ in the culture supernatants was calculated by using a linear regression equation obtained from the absorbance values of the standards according to the manufacturer\'s procedures.

Immunization and protection experiments {#s2e}
---------------------------------------

Female 6-week-old BALB/c mice were immunized by the intraperitonealwas assessed by SDS-PAGE ([Figure 1](#pone-0029552-g001){ref-type="fig"}) and the concentration was quantified (data not shown). In the end, 35 proteins were successfully purified and qualified for subsequent analysis of their abilities to stimulate T-cell responses ([Table S1](#pone.0029552.s001){ref-type="supplementary-material"}).

![SDS-PAGE of the recombinant proteins.\
The expression of recombinant fusion proteins were analyzed by SDS-PAGE. M, molecular size protein markers; lane 1--12, the purified fusion proteins: BAB1_0063, BAB1_0116, BAB1_0381, BAB1_0512, BAB1_0553, BAB1_0560, BAB1_0597, BAB1_0722, BAB1_0812, BAB1_0917, BAB1_1108, BAB1_1124.](pone.0029552.g001){#pone-0029552-g001}

Proteins that induce IFN-γ secretions in splenocytes of S19 immunized mice {#s3b}
--------------------------------------------------------------------------

To assess the T-cell immunity inducing activity of the 35 proteins, sensitized splenocytes were firstly prepared. BALB/c mice were i.p. immunized with live vaccine strain S19. On the 28th day post the immunization, sera from immunized mice were collected and the IgG levels were measured by


AsnC induce protective immune responses against *B. abortus* 544 challenge {#s3c}
-----------------------------------------------------------------------------------

Then, the 7 proteins were used to immunize BALB/c mice and their protection efficiencies were evaluated. At 45 days after the first immunization, the vaccinated mice were challenged with virulent strain *B. abortus* 544, and 30 days post the challenge, *Brucella* in spleen were isolated and enumerated. Protection efficiency was defined as the difference between the numbers of viable bacteria recovered from spleens of immunized mice and those recovered from spleens of mice receiving PBS. Mice immunized with BAB1_1316 (CobB) or BAB1_1688 (AsnC) plus adjuvant exhibited a significant degree of protection (P\<0.001) when compared with controls receiving PBS, with 1.16 and 1.89 units of protection, respectively ([Table 2](#pone-0029552-t002){ref-type="table"}). As expected, the known protective antigen Cu-Zn SOD and theto *Brucella* [@pone.0029552-Eze1], [@pone.0029552-Zhan1]. Thus identification and characterization of *Brucella* antigens which present T-cell epitopes to the host will contribute greatly to development of subunit vaccines against *Brucella* infection.

The availability of complete bacterial genome sequences makes a push for reverse vaccinology to be put into practice [@pone.0029552-Masignani1], [@pone.0029552-Mora1]. Immunoproteomics and protein microarrays were used to identify novel immunogens that induced humoral immune responses in a high-throughput manner [@pone.0029552-Yang1], [@pone.0029552-Altindis1], [@pone.0029552-Chen1], [@pone.0029552-Doolan1], [@pone.0029552-Li1]. However, the approach to identify T-cell antigens on a large scale is just being underway [@pone.0029552-Yang1], [@pone.0029552-Covert1]. In this study, proteins associated with *Brucella* pathogenesis were expressed in *E. coli*. Then, their abilities to induce T-cell responses were tested. For the proteins that could stimulate strong IFN-γ responses, immunogenicity and protective ability were further evaluated. This


issue where Microsoft Edge and / or the taskbar stop responding. If that happens, please open Task Manager (Ctrl + Shift + Esc) and try closing Microsoft Edge and restarting explorer.exe.

Known issues for Mobile

Speech packs will be unable to download on this build.

Adding a new card / paying with an existing card will not work in Microsoft Wallet.

Community Updates

We spent last week at Ignite Australia for the Windows Insiders Program for Business announcement. For more details, please see Bill Karagounis’ blog on it.

We’re so happy to see such interest from all of you around wanting to run Insider builds in your enterprises and to connect deeper with other communities of IT Pros. You’ll be hearing a LOT more from us in the next few months about how wemaps of mars than the 50% of total US territory which lies under the sea

most of the southern hemisphere's underwater terrain is unexplored (more exploration ships in that region during captain cook's times than today)

the seabed is harboring countless well preserved archaeological artifacts

although most of the ocean does not get exposed to photons from the sun, and hence there is no photosynthesis, it is completely erroneous to not expect life

the antarctic sea is, unexpectedly and only recently discovered, teeming with marine life

hydrothermal vent systems are islands of life

bacteria replicating photosynthesis in the dark by chemosynthesis where accidental discovery as no one ever predicted their existence

resilient bacteria living in ph 11 environments

7 new species found per hour of deep reef exploration

2000 to 2500 estimated new species in the indo-pacific


ocean alone (vs. 5000-6000 known species)

not only new species are still being discovered, but also new behavior and new ecology

amazing variety of bioluminescent animals

fact iii: the seas are being destroyed at breakneck speed

90% of all big fish have disappeared in the last 100 years

50% of all coral reefs are destroyed

for 1kg of fish ending up in a market, at least 10kg of bycatch was killed and thrown away

1.7% of the blue whale population is remaining

10% of the tuna fish population is remaining

commercial trawlers are forced to access ever deeper seabed due to the depletion of fisheries; this completely destroys potential habitats which are totally unexplored and eradicates possibly unknown species

throwaway plastic accumulating in the sea (half of the 100 billion pounds of plastic pellets a year are madeit stop being fun for you?

Nobody expected [“The Voice”] to be as big as “Idol” or take off the way it did. It just became a whole different kind of a machine. You’d have two teams at once because they were overlapping seasons. It just wasn’t exactly what I wanted to be doing with my life. I’m not a spokesperson. I’m an artist.

The blind audition thing was very intriguing to me because it provided an opportunity for anybody to get on stage and be discovered, regardless of their look. Being in this business for so long and knowing how labels work and how packaging is so very important, that idea of not being able to see them was genius to me. But year by year, I kept seeing


The stapedius reflex test studied with laboratory and commercial equipment in acoustic neurinomas.
Comparisons were made of contralateral stapedius reflex recordings obtained by means of two different electroacoustic impedance devices, the commercial Grason-Stadler 1723 (G-S 1723) impedance meter and a laboratory equipment, in patients with acoustic neurinoma. With the latter, acoustically elicited reflex responses were noted in 11 of the 21 tumour ears studied. The recordings with the G-S 1723 showed deflections indicating stapedius reflex responses in all 21 cases. Three of these deflections proved to be artefacts. The stapedius reflex threshold test indicated a retrocochlear lesion in all 21 pathological ears when the laboratory equipment was used. The G-S 1723 produced recordings indicating a cochlear lesion in two of these tumour ears. Furthermore, five of 15 normal contralateralIonotropic glutamate receptors in Caenorhabditis elegans.
Ionotropic glutamate receptors (iGluRs) are an important class of heteromeric ligand-gated receptor complexes that mediate a large portion of the excitatory neurotransmission in the vertebrate CNS. Since the cloning of the first iGluR subunit in 1989, the study of this receptor family has rapidly developed in mammals and expanded to include the study of conserved glutamate receptors in simpler invertebrate systems, including the fruit fly Drosophila melanogaster and the soil nematode Caenorhabditis elegans. These model organisms have enabled the genetic analysis of glutamate receptors in the context of a simpler nervous system and provided new insights into receptor function and regulation. In this review we will focus on recent studies that have used genetic, behavioral, and electrophysiological techniques to study the function of


gospel group in the park. It was a fun filled day. I’m a little tired, but that is ok.

My Pearl today is the ability to get up and walk and move in order to enjoy God’s creation.

Pilate said, ” So you are a king?” Jesus responded, “You say I am a king. Actually, I was born and came into the world to testify to the truth. All who love the truth recognize that what I say is true.”

This is interesting statement made my Jesus. If I love truth I will recognize when the Lord speaks the truth or others speak truth.

I would imagine the same is true for lies. If I love truth then I would notice lies, as well.

Today we got up before the chickens were upGov. Phil Murphy and Newark Mayor Ras Baraka announced a strategic partnership between the Newark Alliance and Choose New Jersey on Wednesday that will position and highlight Newark as a major driver of the state’s branding and corporate attraction efforts.

The partnership will seek to align mutual corporate attraction, marketing and branding efforts.

Choose New Jersey and the Newark Alliance will share resources dedicated to research, policy or other agreed-upon priority areas; data sets and analytics; alignment on a comprehensive marketing and branding campaign; the development of corporate attraction strategies and tactics; and the identification of priority areas for real estate development and collaboration around corporate engagement opportunities, including member events and programs, the groups said.

In addition to the partnership, Choose New Jersey will be implementing its “ChooseNJ 2.0” strategy,


the city and state in a number of ways.

“This partnership will allow us to greater position the region for talent attraction and retention, strengthen the state brand, and promote its assets to outside companies and potential employees alike,” he said in a statement.

“With the alignment and collaboration of our organization and Newark Alliance, both the state and the city will see great benefit from our individual and combined efforts.”

Those efforts will include the Newark Alliance launching a comprehensive marketing and branding campaign for Newark, focusing on job-intensive opportunities and promoting the city as an ideal headquarters location to site selectors, the Alliance said.

The organization also will be working to identify priority areas for real estate development, while developing a robust talent pipeline through its existing programs such asExpression of human factor IX gene in murine plasma through lentiviral vector-infected haematopoietic stem cells.
1. Haematopoietic stem cells (HSC) are an attractive target for gene therapy. Gene transfer to HSC can provide a potential cure for many inherited diseases. Moreover, recombinant lentiviral vectors can transfer genes efficiently to HSC. In the present study, we used the recombinant lentiviruses FUGW (Flip, ubiquitin promoter, GFP and WRE vector) and FUXW (Flip, ubiquitin promoter, F IX and WRE vector), which carry the enhanced green fluorescent protein (EGFP) and human factor IX (hFIX) gene, respectively, to infect HSC. 2. High titres of recombinant lentivirus were prepared from 293T cells by calcium phosphate-mediated transient cotransfection. Murine mononuclear cells (MNC) separated from murine bone marrow and HSC separated by magnetic cell sorting were cultured


The genetic diversity of European type PRRSV is similar to that of the North American type but is geographically skewed within Europe.
Porcine reproductive and respiratory syndrome virus (PRRSV) is a recently emerged pathogen. Two PRRSV genotypes exist, North American and European, which are only 55-70% identical at the nucleotide level. Previous studies have shown high nucleotide diversity in the North American genotype and low nucleotide diversity in the European genotype. Here, we analyzed the ORF5 and ORF7 genes for a large number of new European type PRRSV isolates in conjunction with existing database sequences. This new analysis showed that contrary to previous assumptions, genetic diversity is at least as high in the European genotype as in the North American genotype. Furthermore, we showed that genetic diversity of European3 s window hypothesis. The small amount of supportive evidence is better interpreted as effects of specific processing mechanisms, not as showing general temporal integration. The evidence shows that temporal integration occurs on multiple time scales and no particular duration is special, and that windows of temporal integration are defined in terms of information density, not in terms of duration. The subjective present is constructed through local temporal integration on multiple time scales, further integrated into a coherent global representation of what is going on. (PsycINFO Database Record


Upgrades Unveiled at the Alamodome

By Pilar Arias

The newly reimagined Alamodome was unveiled Wednesday.

The $60 million renovations marks the most significant capital improvement project in the Dome’s 24-year history.

“Most of them are technology. (We’ve) put in two new video boards, we’ll eventually have four new video boards by the end of the year,” Convention and Sports Facilities Department Director Mike Sawaya said. “New sound system, ribbon boards so the experience itself overall is going to be very dynamic.”

The investment was made to attract next year’s NCAA Men’s Final Four Championship, with hopes of getting other big name events.

“We expanded the concourses, doubled the size for flow throughout the building so it really is going to be a different experience for our people here in San Antonio,” Sawaya said.

Mayor RonQ:

Positive correlation between reputation score and answer/question ratio?

I only recently learned about meta-stackoverflow and have now read over many questions and answers here. First off, let me say I do not make my employment by programming. I do biomedical research and programming is my hobby so my reputation on any SE site has no meaning to me other than as an indication that I'm contributing to the community. I love SO. It is one of the coolest things I've come to learn about in the last 2 years. However, it is disheartening to realize how successfully most of the people with the highest reputations argue in favor of the answer over the question.
I know that people who ask lots of questions might be annoying, https://meta.stackexchange.com/questions/23404/whats-the-deal-with-user-shore. But I have


them how to act professionally; they knew how instinctively.
Today, without strict controls, I can tell you with absolute certainty that the site would literally be flooded with terrible questions.  How do I know this?  Because it's happened before.  Those were the dark days, when the great unwashed had finally realized that Stack Overflow was the place to be.  When every question on the front page was an indecipherable mess.  Before the automated filters were put into place.  We literally had to throw a machine at the problem to control the onslaught.
Does that sound elitist?  Maybe.  But the folks who come here without the slightest bit of respect about what it is that we're trying to do here are the reasonBackground
==========

Uracils in DNA may arise either from incorporation of dUTP in place of thymidine 5\'-triphosphate (dTTP) or from the generation of uracils in DNA consecutive to spontaneous or enzymatic deaminations of cytosines which, if unrepaired, will lead to non-mutagenic U:A or mutagenic U:G mispairs, respectively. Although U:A mispairs resulting from excess of cellular dUTP pool levels are not mutagenic *per se*, they elicit a cycle of dUMP incorporation into DNA followed by the removal of uracil base by cellular uracil DNA glycosylases (UNG) and reincorporation of dUMP during the synthesis phase. The end point of this process is the appearance of strand breaks and the loss of DNA integrity. In nonproliferating cells such as macrophages, quiescent lymphocytes or neurons the intracellular deoxynucleotide pool is low and imbalanced, with


in contrast to its Old World monkey (OWM) counterpart, may be explained by a poor expression \[[@B26]\]. The antiviral effect displayed by other human APOBEC3 proteins, with the exception of APOBEC3A, was associated with numerous cytosine deaminations (known as \"editing\") within the viral cDNA leading to lethal G-to-A mutations (reviewed in \[[@B25]\]). Indeed, APOBEC3A has been found to exert antiviral effects without cytosine deaminations although recent reports showed that it was capable of editing *in vitro*on single-stranded DNA \[[@B27]\] and on the cDNA of the avian alpharetrovirus RSV thereby inhibiting its infectivity \[[@B28]\]. Studies of deaminase-defective APOBEC3 mutants have shown that APOBEC3G and APOBEC3F contain antiviral determinants that can act independently of the editing process \[[@B29],[@B30]\]. However, a recent study reported that this previously described antiviral effect ofcould however recognize U:G mispairs and could be required to generate A:T mutations \[[@B51]-[@B54]\]. A recent study has indicated that the MSH2-MSH6 heterodimer could prevent the error-free BER commonly initiated by UNG2 and that UNG2 could recruit pol η, which appears to be the sole contributor of A:T mutations \[[@B55]\]. Thus, UNG2 plays a key role in SHM as well as CSR processes. Indeed, UNG2-deficient humans cannot ensure CSR and therefore have elevated IgM amounts and dramatically lowered IgG, IgA and IgE levels \[[@B46]\]. However, the role of the uracil excision activity of UNG2 in this process seems not clear and warrants further studies. Altogether, these data highlight the crucial role of UNG2 in the adaptative immunity against numerous pathogens, like bacteria or viruses.

Numerous viruses have evolved strategies


low level of cellular UNG2 \[[@B62],[@B63]\]. The gamma herpesviruses, like Epstein-Barr viruses (EBV), Kaposi\'s sarcoma-associated herpesviruses (HHV-8) or murine γ-herpesvirus-68 (MHV-68), also encode a functional UNG and dUTPase \[[@B64],[@B65]\]. The EBV-encoded UNG was shown to ensure the fidelity of viral DNA replication and to promote efficient production of viral DNA \[[@B64]\] and the EBV-encoded dUTPase expressed by MHV-68 was found to play an important role in acute infection in the lung \[[@B66]\].

The members of the *Poxviridae*family, such as vaccinia virus, replicate their dsDNA exclusively in the cytoplasm of infected cells. Thereby, due to the total absence of cytosolic UNG activity, the inactivation of the virally-encoded UNG leads to an impaired viral replication even in proliferating cells \[[@B67],[@B68]\]. These data highlight that uracils in the genome of DNA virusesin-between reverse transcriptase and integrase, or downstream of integrase, indicating that retroviruses have captured a *dUTPase*gene during independent occasions. This suggests that the need of the dUTP hydrolysis activity is crucial to confer a selective advantage for retroviral replication. The virally-encoded dUTPase protein is expressed as a part of the Gag-pol polyprotein precursor and is expected to be encapsidated into viral particles in order to be in close spatial proximity of the reverse transcriptase \[[@B69]\]. Although the role of β-retroviral or endogenous retroviral dUTPases still remains to be fully determined, the study of the dUTPase of nonprimate lentiviruses revealed that it is required for efficient replication only in non-dividing cells, \[[@B70]-[@B74]\]. It is noteworthy that other retroviruses members, such as murine leukemia virus (MLV) related viruses or avian


shown that Vpr could participate in the fidelity of reverse transcriptase \[[@B110]\], the role of the Vpr-UNG2 association remains to be evaluated. Interestingly, recent results showed that the expression of Vpr in B-cells had a dominant-negative effect on the class switch recombination (CSR) events \[[@B117]\]. This effect appeared to be dependent on the interaction of Vpr with the WxxF motif contained in UNG2 suggesting that Vpr could compete with some endogenous Vpr-like factors important for UNG function in CSR. Indeed, the CSR is initiated by AID and the AID-induced uracils are removed by a UNG2- or a MSH2-dependent mechanism \[[@B118]\], leading to a not fully characterized dsDNA cleavage step required for CSR (reviewed in \[[@B119]\]). By analogy, it is possible that the Vpr-UNG2 complex may play a roleimmune escape processes of HIV-1. Likewise, UNG2 encapsidation level within viral particles seems to be sufficient to prevent the dUTP incorporation in viral reverse transcripts. However, nothing is known about the efficiency of HIV-1 replication when infected cells exhibit high levels of intracellular dUTP. Therefore, it may be interesting to test whether enhancing artificially the dUTP pool levels in HIV-1 infected cells may represent an alternative approach to antiviral therapy. This could be done by inhibition of thymidylate synthase or dUTPase. The development of drugs against these enzymatic activities may deserve to be evaluated as a viable therapeutic approach.

List of abbreviations
=====================

dUTP: deoxyuridine 5\'-triphosphate nucleotide; AID: activation-induced cytidine deaminase; APOBEC: apolipoprotein B mRNA editing catalytic polypeptide; UNG: uracil DNA glycosylase; dUTPase: deoxyuridine 5\'-triphosphatase nucleotide hydrolase; BER: base excision repair;


is this suppose to be a card trick?’’

Another time involved me hiding in my bedroom closet and coming out of it. My friend and I had been talking about ghosts and haunted houses in town (she’s absolutely petrified of ghosts, so maybe it wasn’t the best idea). So when I went and hid in my closet and texted her to come in my room, she thought I was pretending to be a ghost and was trying to scare her. After convincing her that that was not my intent, she sat down on my bed and I came out of my closet. Again, my heart was pounding, hopeful to be received by a smile and a hug, but all I saw was a blank look on her face. ‘‘Ilife. And I believe he lives it well.

"Track coaches often form very strong bonds with their athletes, almost like they are our adopted children – I feel very fortunate and proud to have such a wonderful 'track son.' I am very proud of all he accomplishes and very grateful that I have been able to be a part of his life."

I also told teammates that I was writing this article and wanted to let them know before it was published. My coming out to one of my teammates that I find of particular interest happened after our annual university sports banquet. After the banquet, an alumni from our team who I still trained with asked me why I hadn’t confided my sexual orientation to him.

I froze at this


for this teammate, but because I was receiving support from the type of person I was the most reticent of coming out to when I was younger (most of the homophobic comments I heard growing up came from other male athletes or male figures in the community).

I learned a lot about myself and about others during the coming out process and that most of my preconceived notions when I was younger were wrong. Coming out didn’t tear down my relationships with others but made them stronger, and the people that matter will see you for the content of your character and the quality of friendship you have with them rather than your sexual orientation. I hope that, by sharing this, someone can relate to my story, that itprotect you from weight gain think again! Studies show increased risk for cancer, diabetes, neurological disorders, and weight gain! They actually cause what you want to prevent; weight gain! Glucose dysregulation occurs when you eat artificial sweeteners. They change the composition of your gut bacteria leading to altered glucose metabolism. This means glucose levels in your body rise higher than normal after eating and decline more slowly than normal leading to weight GAIN vs weight loss. These sweeteners also stimulate your appetite! Sugar does not.

The only natural sweetener with no negatives is Stevia. It comes from a plant, is 200-300x sweeter than table sugar and has no calories.

The experts I study under agree that “a little” sugar used so you will eat and enjoy healthy starches is perfectly


modulators that could potentially influence the MYC mRNA stability. One of them, APOBEC3B, is closely related to APOBEC1, which has been well characterized as a RNA-editing enzyme capable of binding MYC mRNA in the 3’ untranslated region, thus increasing its stability [@kw:Ana2000]. While APOBEC1 was excluded from our analysis due to its insufficient expression range, the identification of its closely related family member, APOBEC3B, may suggest a similar mechanism. Another protein from this category, HNRPDL, encodes a heterogeneous nuclear ribonucleoprotein which interacts with mRNA and may have a role in mRNA nuclear export.

MYC stimulates gene expression in part at the level of chromatin, through its association with co-factors that affect the histone acetylation and DNA methylation. DNMT1, which encodes a DNA methyltransferase, was found in our putative modulator list.N. Inferring cellular networks using probabilistic graphical models. [*Science*]{} [**303**]{} (2004) 799–805.

Gardner, T. S. and di Bernardo, D. and Lorenz, D., Collins, J. J.: Inferring genetic networks and identifying compound mode of action via expression profiling. [*Science*]{} [ **301**]{} (2003) 102–105.

Elkon, R., Linhart, C., Sharan R., Shamir, R., Shiloh, Y.: Genome-Wide In Silico Identification of Transcriptional Regulators Controlling the Cell Cycle in Human Cells. [*Genome Res.*]{} [**13**]{} (2003) 773–780.

Stuart, J. M., Segal, E., Koller, D., Kim, S. K.: A gene-coexpression network for global discovery of conserved genetic modules. [*Science*]{} [**302**]{} (2003) 249–55.

Basso, K., Margolin, A. A., Stolovitzky, G., Klein, U., Dalla-Favera, R., Califano, A: Reverse engineering of regulatory networks in human B cells. [*Nat. Gen.*]{} [**37**]{} (2005) 382–390.

Zeitlinger, J., Simon, I., Harbison, C. T., Hannett, N. M., Volkert, T. L., Fink, G. R., Young, R. A.: Program-Specific Distribution of a Transcription


didn't I want to go? Perhaps it's a bit desperate? but then again it isn't much different from a blind date or an exchange of telephone numbers in a night-club. Does it matter that he approached me on the street?

How much do you need to know a guy before going on a date? when I start to get to know guys, we become friends; friends that you don't want to date because they have entered the too-much-of-a friend zone.

Guess it all boils down to what you want. There haven't been many times that it has been crystal clear that I want to be with someone. However, irrespective- it's always been disastrous. My luck with men stinks. Perhaps that's why I have my guards up too high.

Hmmm.

I can't callof their students. They offer traditional schools with a full range of curriculum complemented by cultural arts, music, foreign languages, technology, career-technical education, and athletic programs.

Montgomery Public Schools takes advantage of the Video Module to keep parents informed of board meetings they may not be able to attend.

Carrollton City Schools/Athletic Site

Carrollton City Schools has been a SCHOOLinSITES customer since 2008. They not only serve the children of Carrollton, GA but others who have opted to become part of the tradition of excellence the school system has enjoyed since its establishment in 1886.

Among the school system's amenities is a centralized 130-acre campus that houses four schools serving more than 4,500 students: Carrollton High School, Carrollton Junior High, Carrollton Middle School and Carrollton Elementary. The Carrollton City Schools and community


September 1918. The advance shown was actually made by combined American and Australian forces.

Show Your Support – We Don’t Believe in Disruptive Ads

Semper Fidelis

Always Faithful, Always Forward

Places to find me:

StackExchange Google+

Twitter : Follow GitHubFollow @GuardianMajor ello deviantArt Facebooki have made a personal choice after their "name policy" witch hunt which repeats every 2 years it seems at the whim of the "bully mob" (even when they make you jump through hoops and verify you), to just quit it and be done with it, they are not worth my time. I don't need it, I don't miss it, in fact it has made my life more productive and void of gross hate, vitriol and drivel. To those who say they can't stay in touch if I am not onfrom each vegetation cover in each sampling year. The soil samples were stored in black plastic bags at 4 °C until subsequent laboratory analysis.

Moisture and pH
---------------

Soil pH was measured in deionized water (soil/solution, 1:2 w:v) with a digital pH meter (Corning™). A subsample of 100 g was oven-dried at 75 °C to constant weight for soil moisture determination using the gravimetric method.

Biogeochemical analyses
-----------------------

### Nutrient analysis

All Carbon (C) forms analyzed were determined with a Total Carbon Analyzer (UIC Mod. CM5012; Chicago, USA), while nitrogen (N) and phosphorus (P) concentrations were determined by colorimetric analyses, using a Bran Luebbe Auto Analyzer III (Norderstedt, Germany). Microbial P and enzymatic activity were determined by colorimetric analyses using a spectrophotometer Evolution 201 (Thermo Scientific Inc.).

### Total nutrients

Prior to analysis of total nutrient forms, soil samples


with annual precipitation (*R*^2^ = 0.76 and *R*^2^ = 0.88 for RS and G, respectively).

10.7717/peerj.4007/table-1

###### Means and (standard errors) of soil nutrients and ratios in the rosetophylous scrub (RS) and grassland (G) soils over three consecutive years (2012, 2013 and 2014) in the Cuatro Ciénegas Basin, Coahuila, Mexico.

Different uppercase letters (A and B) indicate significantly different means (*P* \< 0.05) between vegetation cover types (rosetophylous scrub and grassland) within the same sampling year (2012, 2013 and 2014); whereas different lowercase letters (a, b and c) indicate significantly different means (*P* \< 0.05) among sampling dates within the same site.

![](peerj-05-4007-g006)

                                                                             17 (5)^Ac^      9 (5)^Ac^       79 (6)^Ab^       48 (6)^Bb^       127 (0.2)^Aa^    63 (4)^Ba^
  Nmic:Pmic                                          0.9 (0.3)^Ac^   0.4 (0.2)^Ac^


required investment of energy towards the acquisition of P in order to produce ecoenzymes ([@ref-23]; [@ref-55]; [@ref-81]). The microbial C:N:P ratio was greater in the RS soil (127:19:1) than in the G soil (63:10:1), suggesting that the microbial community in the former site is more P-constrained ([@ref-13]). The studied soils are characterized by low P availability and a high capacity for P occlusion within inorganic molecules, mainly by Ca-bound ([@ref-52]). Therefore, the main source of available P is mineralization of organic P mediated by phosphatase activity ([@ref-82]). Among organic P molecules, phosphodiester forms are the preferred substrate in P-limited ecosystems ([@ref-38]; [@ref-71]), although phosphomonoester forms may also be an important source of available P in most soils ([@ref-76]). In our study sites, phosphodiesterase activity was almost ten timeshigher than that of phosphomonoesterase, mainly in the scrub soil, suggesting mineralization of phosphodiesters as the main source of soil available P. Several bacteria isolates from CCB soils prefer to grow in DNA as a P source, associated with phosphodiesterase activity ([@ref-71]). We suggest that the main P source in sites with low nutrient availability, such as the RS soil, is recycling of the organic molecules that are the product of cellular lysis.

However, the G soil had higher enzymatic activity of polyphenol oxidase in the wet year 2014 than was the case in the RS soil. This result is consistent with other studies ([@ref-58]; [@ref-59]), which have reported that polyphenol oxidase activity does not present the same behavior as the *β*-1,4-glucosidase and other hydrolases that degrade labile C.


Ciénegas Basin, Coahuila Mexico.

######

Click here for additional data file.

This paper is presented by Cristina Montiel-González as partial fulfillment of a doctoral degree at the "Programa de Posgrado en Ciencias Biológicas, UNAM". We thank Rodrigo Velázquez-Durán for his assistance during chemical analyses and Angel Bravo-Monzón for his helpful comments on earlier versions of this manuscript. We thank two anonymous reviewers for comments on a draft of the manuscript.

Additional Information and Declarations
=======================================

Valeria Souza is an Academic Editor for PeerJ.

[Cristina Montiel-González](#author-1){ref-type="contrib"} conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables.

[Yunuen Tapia-Torres](#author-2){ref-type="contrib"} analyzed the data, wrote the paper.

[Valeria Souza](#author-3){ref-type="contrib"} contributed reagents/materials/analysis tools, reviewed drafts of the paper.

[Felipe García-Oliva](#author-4){ref-type="contrib"} conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools,Background
==========

Since multiple sequence alignments are the basis of many analyses, for example in phylogenetics or in analysing functional protein domains, there is a need for programs to create and improve those alignments. Currently, several programs are available to fulfil these necessities, e.g., CLUSTAL W \[[@B1]\], MUSCLE \[[@B2]\], DiAlign \[[@B3]\], T-Coffee \[[@B4]\] or DCA \[[@B5]\] all of which are able to align multiple sequences globally. The underlying methods have their strengths and weaknesses, and resulting alignments can diverge from the biologically correct ones. Editors like JalView \[[@B6]\], SEAVIEW \[[@B7]\], CINEMA \[[@B8]\] or Align \[[@B9]\] are needed to enhance the results by hand.

The just mentioned tools are based on sequence information only, but in RNA sequence analyses there is often also structural information available. Databases like the ITS2 Database \[[@B10]-[@B12]\]


was also damaged. At the time, Astoria PD had no idea who hit the cars that belonged to people attending a party, officials said.

Later the same day, Riley received the private Facebook message from someone reporting that they had seen Cox-Brown's post that he had been in a car crash. Police Sgt. Aydt also received a call from another "friend" of Cox-Brown's claiming he also saw Cox-Brown post the status.

Astoria PD released a statement saying, "Aydt and Clausen went to Cox-Brown's house and found a vehicle that matched the damage done to the two vehicles at the early morning crash."

"It's one of those six degrees of separation kind of things," deputy chief of police Johnston told ABCNews.com. "The officer's brother was at the party at the time ofthe crash."

During the interview with KATU-TV, Cox-Brown said he had hit the car as he drove by as a result of icy conditions on the street and not because he was driving drunk.

Cox-Brown has not responded to requests for comment.

Astoria PD arrested Cox-Brown and charged him with two counts of failing to perform the duties of a driver.

He is scheduled to appear in the Astoria Municipal Court Jan. 23.

Police didn't know whether Cox-Brown has an attorney.


Berlin? Here are some facets of city life that many Israelis in Berlin cite as major reasons for loving the former capital of Nazi Germany.In an informal survey of Israelis on the “Israelis in Berlin” Facebook page, transportation rates high as the most attractive Berlin offering. Here’s why the railway system that once efficiently took Jews do their deaths now efficiently takes Jews to everyday life.It is fast, frequent, and on-time. Berlin offers so many ways to get around that a car is not only unnecessary, but a drag to own. The S-Bahn (city railway), U-Bahn (underground), the bus, and the tram all run at peak hours, often every few minutes. Digital schedule displays are usually accurate up-to-the-minute. Furthermore, if a railway is being repaired, the city usuallyTreatment of cellulite with LPG endermologie.
LPG endermologie is a FDA-approved massage system in use worldwide for cellulite treatment that lacks clinical study. To determine the efficacy and safety of LPG endermologie in treating cellulite. Thirty-three healthy women (cellulite grades, 1-3 based on the 4-stage Nurnberger-Muller scale) had LPG treatments twice weekly for a total of 15 sessions. Clinical evaluation was performed by digital photography for cellulite grade assessment, and perimetric measurements of eight body sites for the evaluation of body contours. Significant differences were found regarding mean cellulite grades before and after treatment. However, improved cellulite appearance occurred in only 5 women (15%). All patients showed a significant circumference loss at every measured body site. Weight losers had significantly greater loss of total and average body circumference than


her son, however she doesn’t hesitate to eliminate another person’s child. Who isn’t a precious child to someone else? If she could pull such a stunt on Hou shi, she should also be prepared to bare the consequences.the vertices of the three dimensional pyrochlore lattice of corner-sharing tetrahedra are frustrated. [@Villain; @MoessnerChalker; @MoessnerChalker2; @Reimers; @Reimers2; @GGG] One consequence of this frustration is an extensive (macroscopic) ground state degeneracy and lack of conventional long-range order down to arbitrarily low temperatures. Theoretically, this degeneracy is expected to be lifted, partially, or fully, by other interactions, [@PalmerChalker; @Elhajal] perhaps assisted by the presence of thermal or quantum fluctuations. [@MoessnerChalker2; @Champion; @ChampionHoldsworth] These lessons carry over to real pyrochlore magnets in which the frustration of the principal spin-spin interaction usually manifests itself in a transition to long-range order [@GTO1; @GTO2; @GSO; @Champion] or a spin glass transition [@Gingras3; @Gardner4] well below the temperature scale set by the interactions $-$ the Curie-Weiss temperature $\theta_{{\rm CW}}$. In fact, this is a


state: $$\begin{aligned}
\tilde{\sigma}^{x} = |1\rangle\langle 2 | & + |2 \rangle\langle 1| \\
\tilde{\sigma}^{y} = -i(|1\rangle\langle 2 | & - |2 \rangle\langle 1|) \\
\tilde{\sigma}^{z} = |1 \rangle\langle 1 | & - | 2 \rangle\langle 2|
\label{eqn:Pauli} \end{aligned}$$ together with the unit operator $\mathbb{I} =|1 \rangle\langle 1 | + | 2
\rangle\langle 2|$. Note, however, that despite the fact they do satisfy the commutation rules $$[\tilde{\sigma}^{\alpha}, \tilde{\sigma}^{\beta}] = 2 i \epsilon_{\alpha
    \beta \gamma} \tilde{\sigma}^{\gamma},$$ where $\epsilon_{\alpha \beta \gamma}$ is the Levi-Civita symbol, the $\tilde{\sigma}^{\alpha}$ do not swap sign under time reversal so they are not true angular momentum operators. For this reason, we shall call them pseudospins or effective spins. It is helpful for later sections to give their properties under $\mathfrak{T}$, the time reversal transformation: $$\begin{aligned}
\mathfrak{T}: \tilde{\sigma}^{x} & \rightarrow\tilde{\sigma}^{x} \\
\mathfrak{T}: \tilde{\sigma}^{y} & \rightarrow \tilde{\sigma}^{y} \\
\mathfrak{T}: \tilde{\sigma}^{z} & \rightarrow -\tilde{\sigma}^{z}
\label{eqn:time}\end{aligned}$$ because $\mathfrak{T}:| 1 (2)\rangle\rightarrow | 2 (1)\rangle$.

If we apply the projector to the full Hamiltonian to obtain $\mathcal{P}H\mathcal{P}$, we find that the crystal field part $H_{{\rm cf}}$ becomes $E_{0}\sum_{i,a}\mathbb{I}_{i,a}$ with $E_{1}=E_{2}\equiv E_{0}$. From now on, we omit this constant energy shift. To project the interaction part $\mathcal{P}V\mathcal{P}$, we write the angular momentum components in the local coordinate system with local $\mathbf{\hat{z}}^{a}$ axes in the directions given in Table \[tab:lattice\]: $J^{\alpha}_{i,a}=u^{a}_{\alpha\beta}\widetilde{J}^{\beta}_{i,a}$. All operator components that refer to the local coordinate systems are labeled with a tilde. Also, when it is not important to distinguish different sublattices, we abbreviate $(i,a)$ with the site index $I$. We add further numerical subscripts to $I$ to label different sites. With this


in hand, we write the quantum term $H_{\rm eff}^{(2)}\equiv \mathcal{P}V\mathcal{R}V\mathcal{P}$ as $$\sum_{I_{1}, ..,I_{4}}\sum_{\alpha,\beta,\gamma,\delta} \mathcal{P} \left( \mathcal{K}_{I_{1}I_{2}}^{\alpha,\beta}
 J_{I_{1}}^{\alpha}J_{I_{2}}^{\beta}\right)\hspace{1pt} \mathcal{R} \hspace{1pt}\left(\hspace{1pt}
 \mathcal{K}_{I_{3}I_{4}}^{\gamma,\delta} J_{I_{3}}^{\gamma}J_{I_{4}}^{\delta}\right) \mathcal{P}.
\label{eqn:expanded}$$

There are a few observations that we can make from Eq. (\[eqn:expanded\]) that identify classes of nonvanishing terms. Suppose we choose magnetic sites $I_{p}$ on the pyrochlore lattice for $p=1,2,3,4$ in Eq.(\[eqn:expanded\]). Then, when we evaluate Eq.(\[eqn:expanded\]) for all other sites, we obtain unit operators $|1_{I_{m}}\rangle\langle
1_{I_{m}}|+|2_{I_{m}}\rangle\langle 2_{I_{m}}|$ for all sites $I_{m}$ with $m\neq 1,2,3,4$. This follows because the resolvent operator $\mathcal{R}$ and projection operators $\mathcal{P}$ are diagonal on each site. In the following, we do not write out all these unit operators explicitly. A second observation is that when we consider a term with magnetic sites $I_{p}$ ($p=1,2,3,4$) all different, we find that such a term vanishes.1_{I_{1}}| \widetilde{J}_{I_{1}}^{z}|1_{I_{1}} \rangle^{2} + |2_{I_{1}} \rangle\langle
  2_{I_{1}} | \langle 2_{I_{1}} | \widetilde{J}_{I_{1}}^{z} |2_{I_{1}} \rangle^{2} \\ = \langle
  \widetilde{J}_{I_{1}}^{z}\rangle^{2}\mathbb{I}_{I_{1}}  \end{gathered}$$ $-$ the unit operator acting on $I_{1}$, which we can omit in the following. When the sum is performed over excited crystal field states on ion $I_{2}$, the resulting operators map to the unit operator $\mathbb{I}_{I_{2}}$ and Pauli matrices $\tilde{\sigma}^{x}_{I_{2}}$ and $\tilde{\sigma}^{y}_{I_{2}}$ $-$ all the resulting operators being consistent with time reversal. This calculation is performed along the lines described in Appendix \[sec:calc\].

It would thus seem that, together with the constant energy shift, there are effective nontrivial single-site transverse field operators in the effective Hamiltonian of the form $\tilde{\sigma}^{x}$ and $\tilde{\sigma}^{y}$. However, these effective transverse field terms cancel on any given lattice site when one sums


the contributions to these single-site operators coming from the bare microscopic pairwise interactions in $V$. Without going into the details of the sum over different contributions to site $I_{2}$ we see that there must be such a cancellation because neither the original model nor the effective Hamiltonian formalism distinguishes particular directions (as opposed to particular axes) on individual sites. When the Tb$^{3+}$ ions are randomly diluted with nonmagnetic ions, as in (Tb$_{p}$Y$_{1-p}$)$_{2}$Ti$_{2}$O$_{7}$,[@Keren] there is no longer perfect cancellation of the effective single site operators. These effective fields have the effect of splitting the degenerate $|1\rangle$, $|2\rangle$ doublet on each ion for which the cancellation does not occur. So, in fact, the low energy effective theory of the diluted compound (Tb$_{p}$Y$_{1-p}$)$_{2}$Ti$_{2}$O$_{7}$ would be somewhat different than that of the[Tb$_{2}$Ti$_{2}$O$_{7}$]{} could be described by a non-frustrated $[111]$ pyrochlore Ising model, virtual crystal field excitations can render this system a frustrated spin ice one, with additional transverse fluctuations. This is the main result of this paper. Secondly, in what follows, we study the semiclassical spin correlations that these effective interactions produce on a lattice.

Semiclassical ground states {#sec:ground}
===========================

Convention and Procedure
------------------------

The effective Hamiltonian that was discussed in detail in the previous sections has a large number of different effective interactions arising from virtual crystal field excitations (VCFEs) from the ground state crystal field doublet (see Section \[sec:summary\]). As we have seen, to lowest (first) order in the perturbation expansion in $\langle V \rangle/\Delta$, the effective Hamiltonian is an Ising model. The lowest order quantum corrections to $H_{\rm eff}$ include transverse terms between


nearest neighbor spins, three-body interactions, and anisotropic interactions extending beyond nearest neighbors. To gain some understanding of the effect of the extra terms generated by VCFEs on the physics of this system, it is useful to consider a semiclassical spin model with the same interactions as in the effective quantum Hamiltonian.

To obtain the required semiclassical model, we first observe that the effective quantum Hamiltonian should be written in terms of pseudospins one-half. That is, the elementary quantum spins take the form $\hat{S}^{\alpha}=(1/2)\sigma^{\alpha}$ and the quantum Hamiltonian couplings derived in the previous section are rescaled by a factor of four for the bilinear interactions and by a factor of eight for the three-body interactions. This model is the most suitable model to consider from the point of view ofnearest neighbor dipolar interactions to a model in which VCFEs are significant. The results are shown in Fig. \[fig:NNPhaseDiagram\_JDelta\]. As one would expect based on the limiting case $1/\Delta=0$ of spin ice and the results discussed in Section \[sec:4sbl\] for $1/\Delta=0.055$ K$^{-1}$, the all-in/all-out ground states and the two-in/two-out ground states are separated by a wedge of ordered local XY ground states (with continuous degeneracy when three-body interactions are omitted). The range of $\mathcal{J}_{\rm ex}$ over which the wedge extends increases as $\Delta$ decreases. For $\Delta\lesssim 20$ K, the AIAO phase is suppressed entirely because VCFEs increase transverse couplings relative to the Ising couplings (see Fig. \[fig:Couplings\]). We return to this phase diagram in Section \[sec:16sbl\] where we make a comparison of Fig. \[fig:NNPhaseDiagram\_JDelta\] with the ground states on a cubic unit cell


$1/\Delta=0.055$ K$^{-1}$ and $\mathcal{D}=0.0315$ K; the values appropriate to [Tb$_{2}$Ti$_{2}$O$_{7}$]{}. In the same figure, we also plot, for comparison, the energies of various imposed (fixed) spin configurations. As in the single tetrahedron case, it is useful to distinguish the ground states obtained when three-body spin interactions are removed and the ground states for the model with all interactions included.

Without three-body spin interactions (open squares in the main panel of Fig. \[fig:Cubic\_Dipole\]) we find that for weakly antiferromagnetic $\mathcal{J}_{{\rm ex}}\lesssim 0.06$ K, the ground state is the LRSI$_{001}$ state. But, for more antiferromagnetic $\mathcal{J}_{{\rm ex}}$, instead of the all-in/all-out state found for the DSIM (see inset of Fig. \[fig:PhaseDiagram\]), the ground state, at least up to $\mathcal{J}_{{\rm ex}}=0.4$ K, is a state with identically ordered tetrahedra (ordering wavevector $\mathbf{q}=0$) eachconsidered, similarly to what was found in Section \[sec:4sbl\] on a tetrahedron. The ordering of the Ising components of the spins is the same regardless of whether three-body interactions are present or not. The canting angle of the spins away from the Ising directions is shown as the inset in Fig. \[fig:Cubic\_Dipole\]. There is a maximum in the angle at about $\mathcal{J}_{\rm ex} =0.11$ K and two minima at about $0.02$ K and $0.21$ K over the explored range of $\mathcal{J}_{\rm ex}$. The greatest angle is about $8$ degrees, compared to $90$ degrees in the case of a single tetrahedron. Broadly, the variation in the canting angle follows the magnitude of the three-body coupling (shown for a choice of three sublattices in Fig. \[fig:Couplings\]). The minimum in the canting angle at


crystal field doublet. The nonvanishing matrix elements within this doublet are given in Eq. (\[eqn:ME\]). From this equation, we see that $\alpha$ and $\sigma$ must equal $z$. We consider the operators in Eq. (\[eqn:operators\]) that act on site $I_{1}$ $$\begin{gathered}
 \sum_{m_{1},m_{4}} |m_{1}\rangle\langle m_{1}| \widetilde{J}_{I_{1}}^{z} | m_{4}\rangle\langle m_{4}| \\ \longrightarrow
  |1\rangle\langle 1| \langle 1| \widetilde{J}_{I_{1}}^{z} | 1\rangle + |2\rangle\langle 2| \langle 2| \widetilde{J}_{I_{1}}^{z} |
   2\rangle \\ = - \langle \widetilde{J}_{I_{1}}^{z} \rangle  \left(|1\rangle\langle 1|- |2\rangle\langle 2| \right) \rightarrow - \langle \widetilde{J}_{I_{1}}^{z} \rangle \tilde{\sigma}_{I_{1}}^{z}.    \end{gathered}$$ A similar calculation gives $- \langle \tilde{J}^{z} \rangle\tilde{\sigma}^{z}$ on site $I_{3}$. Eq. (\[eqn:operators\]) becomes $$\begin{gathered}
\sum_{\beta,\rho}\tilde{\mathcal{K}}_{I_{1}I_{2}}^{z \beta} \tilde{\mathcal{K}}_{I_{2}I_{3}}^{\rho z} \langle \widetilde{J}^{z}\rangle^{2} \tilde{\sigma}_{I_{1}}^{z}
\tilde{\sigma}_{I_{3}}^{z} \\ \times  \left( \sum_{m_{2}, m_{5}} \sum_{W}   |m_{2}\rangle\langle m_{5}| \frac{ \langle m_{2} | \widetilde{J}_{I_{2}}^{\beta}|W\rangle\langle W |
\widetilde{J}_{I_{2}}^{\rho}|Cancers of the cervix and endometrium affect 48,000 women per year. In spite of major advances in diagnostic x-ray surgery and radiation therapy, local control of these malignancies continues to be a problem. In 1985, there were 7,400 deaths from cancer of the cervix and 3,200 deaths from cancer of the endometrium. As part of the standard treatment for the more advanced cases, high-does intra-cavitary irradiation is delivered with a tandem and colpostat. This device can be secured in position in the endometrial canal and surrounding the cervix so that local, intense irradiation can be delivered. Still, locale failures continue to plague patients with more advanced local disease. The intent of this application is to obtain support for the development of modifications to current intracavitary applicators used in


plane ${ \mathbb C }^2$ (A similar result has been conjectured in [@Na2]).

In this note, we shall establish a link somewhat different from [@Na2] between equivariant cohomology of Hilbert schemes and Jack polynomials, and then use this to describe the equivariant and ordinary cohomology rings of the Hilbert schemes of points on the surface $X(\gamma)$. We first show that the Jack polynomials can be realized in terms of certain $\mathbb T$-equivariant cohomology classes of the Hilbert schemes of points on [*the affine plane*]{}, and the Jack parameter comes from the ratio of the $\mathbb T$-weights on the two affine lines preserved by the $\mathbb T$-action. In our view, the present construction is conceptually simpler than the original one in [@Na2]. This result is probably not very surprising howeverN\to M$ of algebraic varieties, there is a Gysin homomorphism $f_!\colon H^*_{\mathbb T}(N)\to
H_{\mathbb T}^*(M)$ of ${\mathbb T}$-equivariant cohomology groups. If $N$ is a ${\mathbb T}$-equivariant codimension-$k$ closed subvariety of $M$ and $i\colon N\to M$ is the inclusion map, define $[N]=i_!(1_N) \in H^{2k}_{\mathbb T}(M)$ where $1_N
\in H^0_{\mathbb T}(N)$ is the unit of the algebra $H^*_{\mathbb
T}(N)$.

In this note, we shall consider two types of surfaces with ${\mathbb T}$-actions, and the Hilbert schemes of points on these surfaces. The first one is the complex plane $\mathbb C^2$, while the second is the total space of a line bundle over $\mathbb P^1$. The ${\mathbb T}$-actions on these surfaces are specified in the following two examples.

\[complexplane\] Fix two nonzero integers $\alpha$ and $\beta$ with the same signs. Let $u, v$ be the standard


$v_2$ of $X_2$ by $$\begin{aligned}
 \label{action_X2}
s\cdot (u_2, v_2)=(s^{\gamma-1}u_2, s^{-1}v_2), \quad s \in\mathbb
T.\end{aligned}$$

Let $x_i$ be the origin of $X_i$. Then $X(\gamma)^{\mathbb T}
=\{x_1,x_2\}$. Let $\rho: X(\gamma) \to \mathbb P^1$ be the projection sending $[(b,b_1, b_2)]$ to $[b_1, b_2]$. Let $\Sigma_0
\cong \mathbb P^1$ be the zero section of $\rho$, and $$\begin{aligned}
 \label{sigma12}
\Sigma_1 = \rho^{-1}([0,1]), \qquad \Sigma_2=\rho^{-1}([1,0]).\end{aligned}$$ Then as $\mathbb T$-modules, we have $T_{x_1}\Sigma_1=\theta, \,\,
T_{x_1}\Sigma_0 = \theta^{-1},\,\, T_{x_2}\Sigma_0 =\theta$, and $T_{x_2}\Sigma_2 = \theta^{1-\gamma}$. By the localization theorem, we get $$\begin{aligned}
 \label{Sigma12}
[\Sigma_1]=t^{-1}[x_1],\,\,\, [\Sigma_0]=-t^{-1}[x_1]+
t^{-1}[x_2], \,\,\, [\Sigma_2]= (1-\gamma)^{-1} t^{-1}[x_2].\end{aligned}$$

\(i) Let $X(1)$ be the total space of $\mathcal O_{\mathbb P^1}(-1)$. Using $[(b,b_1,b_2)] \in X(1)$ to denote the equivalence class defined by (\[equi\_cls\]), we define a $\mathbb T$-action on $X(1)$ by $$\begin{aligned}
s \cdot [(b,b_1, b_2)]=[(sb,s^{-2}b_1, b_2)], \quad s \in \mathbb
T.\end{aligned}$$ Then our methods and results below applyan isomorphism of rings.

The case of the surface $X(\gamma)$ {#sub:line-bundle}
-----------------------------------

$\,$

Let $X = X(\gamma)$ be the surface from Example \[linebundle\]. Recall that there is a $\mathbb T$-equivariant identification between the affine open subset $X_1$ of $X$ and the complex plane in Example \[complexplane\] with $\alpha = \beta = -1$; similarly for $X_2$ and the complex plane in Example \[complexplane\] with $\alpha = \gamma-1$ and $\beta=
1$. By the discussions in Subsection \[general\], $\mathbb
H_{X}$ is the Fock space of the Heisenberg algebra generated by $\mathfrak p_i([\Sigma_1])$ and $\mathfrak p_i([\Sigma_2])$ with $i \in { \mathbb Z}$, where $\Sigma_1$ and $\Sigma_2$ are the two $\mathbb
T$-equivariant fibers in ${X}$ defined by (\[sigma12\]). Note also that $\mathbb H_{X_1}$ is the Fock space of the Heisenberg algebra generated by $\mathfrak p_i([\Sigma_1])$ for $i \in { \mathbb Z}$, where $\Sigma_1$ is


to the basis $\{[\mu^1,\mu^2]\}$ via the transition matrix $M_2$, where the product is explicitly known. Finally, we reverse the above steps and pass to the associated graded ring to express the product of $\mathfrak Q_{\lambda^1, \lambda^2}$ and $\mathfrak
Q_{\mu^1, \mu^2}$ in terms of the Heisenberg monomial basis (\[bas1\]) of $H^*({ X^{[n]}})$.

[ABCD]{}

D.A. Cox, S. Katz, [*Mirror symmetry and algebraic geometry*]{}, Mathematical Surveys and Monographs [**68**]{}, American Mathematical Society (1999).

G. Ellingsrud, S.A. Strømme, [*On the homology of the Hilbert scheme of points in the plane*]{}, Invent. Math. [**87**]{} (1987), 343-352.

L. Göttsche, [*The Betti numbers of the Hilbert scheme of points on a smooth projective surface*]{}, Math. Ann. [**286**]{} (1990) 193–207.

I. Grojnowski, [*Instantons and affine algebras I: the Hilbert scheme and vertex operators*]{}, Math. Res. Lett. [**3**]{} (1996) 275–291.

M. Haiman, [*Vanishing theorems andbusiness.

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"To win the game you need to make clutch pitches," Jurrjens said. "I made my clutch pitches when I needed to. They didn't hit when they had guys in scoring position."

The Mets stranded 12 baserunners.

"Their guy, when he needed a strike, he threw a strike," said Mets manager Jerry Manuel.

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voltage gradient over the ventricles. Others have postulated that uniformity of current density is important since: (i) low gradient areas contribute to the continuation or reinitiation of ventricular fibrillation, and (ii) high current areas may induce temporary damage, that then may cause sensing difficulties, set-up areas of reinitiation of fibrillation, or even potentially cause permanent damage (new arrhythmias, decreased contractility, and myocardial necrosis).
The modern trend in tachycardia pacing has been to use transvenous leads instead of thoracotomy systems. The electrodes in the lead assembly generally consist of two coil electrodes approximately 2 to 4 inches long, one placed in the right ventricle (RV) and the other in the superior vena cava (SVC) or the left brachycephalic vein. The shocking electrodes may be bipolar, in which case only onelead is used, or a combination of two leads, or one endocardial electrode and one subcutaneous patch or epicardial electrode, or two leads and a subcutaneous patch.
Factors that influence the success of defibrillation shocks include the underlying physiclogic substrates of the heart, the pulse waveform of the shock, and the electrode system. In many ways, the physical criteria necessary to design bradycardia electrodes equally apply to tachycardia or defibrillation electrodes.
Two types of lead designs are in common use today, a unipolar (one wire, one electrode) and a bipolar (two wires, two electrodes) lead system. With the unipolar lead, the stimulating electrode is paced against a reference electrode remotely placed from the heart. This reference electrode is usually the metal pacemaker can. For bipolar stimulation, the reference and the


alloys, titanium, titanium alloys, tantalum, and tantalum alloys is coated with iridium oxide to form the electrode. U.S. Pat. No. 4,679,572 discloses an electrode with a conductive tip portion and a substrate composed of a material conventionally employed for pacing electrodes, and a layer of film of iridium oxide overlying the surface of the substrate. The tip portion may be provided with recesses to which the iridium oxide surface layer is confined.
Valve metals have the capacity to conduct current in the anodic direction and to resist the passage of current in the cathodic direction, (i.e., the anodic reaction is irreversible) and are sufficiently resistant to the electrolyte media. In the anodic direction, however, their resistance to the passage of current goes up rapidly, due to the formation ofover the last month for any SDI items. In this paper, only SDI results are reported.

Patients provided sociodemographic information and clinical data were collected from medical notes (age, gender, postcode, diagnosis, date of diagnosis and disease stage). Deprivation levels were determined using Indices of Multiple Deprivation (IMD) based on postcodes and derived from 2001 UK census ([Office for National Statistics, 2001](#bib17){ref-type="other"}; [Noble *et al*, 2004](#bib16){ref-type="other"}).

Interviews
----------

Within a week of the touchscreen assessment, participants were interviewed at home by PW, a social worker investigator, who was blind to the results from the touchscreen assessment. The interview was semistructured, lasted for about an hour and was audio-recorded. The interview concerned the last month and began with a general overview of the patient\'s cancer and cancer treatment followed by more detailed questioning


above the cutoff on overall SD score or '2 -- quite a bit\' or '3 -- a lot\' of difficulty on individual items were identified. The interventions made after the interview (blinded to patient\'s SDI results) were recorded and classified (provision of information, referral to support services). An algorithm for identifying cases is suggested and examples provided using these comparisons.

RESULTS
=======

Participants and non-participants
---------------------------------

One hundred and ninety-one patients of 273 approached consented to participate (70%). Patients in the refusing group were older than participants (*t*=−2.251, d.f.=271, *P*=0.025). The refusing group tended to be less affluent than the consenting group, but this did not reach statistical significance; IMD participants range 2.01 (most affluent) to 75.85 (most deprived), median score=16.76 and non-participants range 2.58 (most affluent) to 73.21 (most deprived), median score=20.29known his views, the trial judge said, inter alia:
"Yes, sir; but it also involves fair play, Mr. Morrow. It involves a full disclosure on the part of the State of the things that they are under order to disclose. Now if you knew of the existence of the photographs, you could at least have told them that Dr. Roper had them and asked them to inquire of Dr. Roper. Now I'm not saying it's all up to you to prepare the Defendant's case, it's not. You're here to prepare the State's case. But at the same time, when we have a conference, pretrial conference and in it we have a Motion to Produce and Inspect and we go down item by item and we rule on each one


of them, then they have a right to rely on what comes out of the pretrial conference." *142 The court stated further, "Sometime between Friday and Monday you could have let them know that Roper had those photographs."
It was the contention of defendant, and is appellant's present contention, that the photographs reveal "that the trajectory of the bullets ... negates the self-defense theory." In commenting on defendant's position, the court said, inter alia:
"... I can't see where really the withholding of these photographs prejudices the Defendant's case. There is nothing you can do about them. They are certainly admissible. The only thing is that you feel rightly or not rightly, and I'm not passing judgment on that, but you feel like you haven't been treated fairly in connectionfrom the motion to produce that it is extraordinarily lengthy and tends to cover almost every conceivable item, but that it lacks specificity. We can see, as appellant contends, the possibility that from a standpoint of advisable tactics there would have been a change in the way counsel prepared and presented the defendant's case if counsel had known before the trial started of the existence of the photographs, but we cannot say that the material evidence as to defendant's guilt or innocence would have been any different from what it was. We can also see that if counsel for the state had intentionally withheld the photographs from inspection by defendant's counsel in order to trap defendant's counsel into an unwise tactical maneuver, defendant's counsel would have just ground


for a more finished-looking product. Examples can be found in U.S. Pat. Nos. 3,657,060 and 3,816,211.
Embroidered appliques suffer from disadvantages including being the most expensive type of applique to produce, in addition to being slow to produce because of the speed of the looms. Moreover, it is difficult or impossible to achieve fine detail in the designs because of the limitations in the stitching process.
Screen printed appliques are made by screen printing textile inks directly onto a textile, and then cutting out the applique. In the alternative, a pre-cut textile applique, with or without a stitched edge, can be screen printed.
Screen printed appliques are perceived as being an inferior products relative to an embroidered applique because they can lack three-dimensionality, rich texture, brilliant appearance, and wash-fastness.
Appliques made byspeed and the quality of the finished design i.e. sharpness of lines separating colors, vivid images, etc., would be adversely affected. It is believed that direct flocking has been limited in use in the United States.
Examples of flock transfers or appliques, i.e., the second method of employing flock fibers in a decorative manner, are illustrated in U.S. Pat. No. 4,142,929, to OTOMINE, U.S. Pat. Nos. 4,292,100 and 4,396,662 both to HIGASHIGUCHI and U.K. Pat. application No. 2,065,031 to MAITLAND and U.K. Pat. application No. 2,126,951 to TRANSWORTH. Transfers are formed by applying flock to a release sheet having a temporary release adhesive coating. The flock is then colored with different color inks and coated with a binding layer and hot melt adhesive in a desired decorative design. The


the oropharynx ([Table 1](#t0005){ref-type="table"}, [Fig. 2](#f0010){ref-type="fig"}A). Total HPV prevalence in TSCC/BOTSCC was 56%, 95% CI: 55--57% (59%, 95% CI: 58--60% for TSCC only) as compared to 19%, 95% CI: 17--20%, HPV prevalence in "other" OPSCC ([Table 1](#t0005){ref-type="table"}).Fig. 2Heat map of HPV prevalence by oropharyngeal cancer sub-site. (A) Prevalence of HPV, defined by each included study, stratified by tonsillar (TSCC) and base of tongue (BOTSCC) squamous cell carcinomas *vs*. "other" (i.e. walls of oropharynx, uvulae and soft palate) oropharyngeal squamous cell carcinomas (OPSCC). (B) Prevalence of HPV, defined by each included study, stratified by TSCC only *vs.* "other" OPSCC.Fig. 2Table 1Studies and their patients included in the meta-analysis.Table 1Author, YearCountry[a](#tbl1fna){ref-type="table-fn"}OropharyngealHPV+HPV-HPVHPVp-value[\*](#tbl1fnStar){ref-type="table-fn"}p-value[\*](#tbl1fnStar){ref-type="table-fn"}sub-sitetumourstumoursprevalencedetection(TSCC and BOTSCC *vs.*"other" OPSCC)(TSCC only *vs."*other" OPSCC)Bahl et al., 2014 [@bib10]IndiaBase of tongue146119% (18--20%)PCRNSNSTonsil101540% (36--44%)Soft palate050% (0--0%)

Bhosale et al.,of AmericaBase of tongue523659% (58--60%)p16 IHC0.020.002Tonsil391967% (66--69%)Soft palate030% (0--0%)Oropharynx NOS14961% (57--65%)Faucial arch010% (0--0%)Pharyngeal oropharynx080% (0--0%)

Faust et al., 2016 [@bib22]SwedenBase of tongue151256% (52--59%)PCR0.001\<0.001Tonsil752873% (72--74%)Oropharynx NOS2918% (11--25%)

Fonmarty et al., 2015 [@bib23]Not specifiedAnterior/lateral oropharynx (*tonsil, base of tongue and glossotonsillar sulcus*)203139% (37--41%)p16 IHC and PCR\<0.001--Other oropharyngeal sites0200% (0--0%)

Fujimaki et al., 2013 [@bib24]JapanLateral272354% (52--56%)p16 IHC and ISHNS0.05Anterior4736% (28--45%)Posterior030% (0--0%)Superior020% (0--0%)

Grisar et al., 2016 [@bib26]BelgiumTongue base63614% (13--16%)p16 IHCNS0.05Tonsil8753% (47--60%)Soft palate1420% (4--36%)Oropharynx NOS82624% (21--26%)

Habbous et al., 2013 [@bib27]CanadaBase of tongue1595076% (76--76%)p16 IHC\<0.0001\<0.0001Tonsil3088379% (79--79%)Other oropharynx275633% (31--34%)

Hama et al., 2014 [@bib28]JapanAnterior62023% (20--26%)PCR\<0.0001\<0.0001Lateral734462% (62--63%)Upper0100% (0--0%)Posterior040% (0--0%)

Henneman et al., 2015 [@bib29]NetherlandsBase of tongue133627% (25--28%)PCR0.0030.001Tonsil374147% (46--49%)Other oropharynx1185% (3--8%)

Hong et al., 2013 [@bib32]AustraliaBase of tongue111838% (35--41%)PCR and p16 IHC0.001\<0.001Tonsil998454% (54--55%)Other oropharynx31814% (11--18%)

Hong et al., 2014 [@bib31]AustraliaBase of tongue293049% (47--51%)PCR and p16 IHC\<0.001\<0.001Tonsil18122245% (45--45%)Other oropharynx104319% (17--20%)

Hong et al., 2013 [@bib30]AustraliaBase of


with HPV positive OPSCC. However, since patients with TSCC and BOTSCC dominate the OPSCC patient group, there is a risk that patients with TSCC and BOTSCC in published survival studies supersede patients with "other OPSCC". This could lead to that patients with "other OPSCC" could disfavour from the introduction of tapered treatment, as well as that de-escalated therapy could be offered to patients with HPV positive "other" OPSCC, where survival benefit is doubtful. Notably, according to the newest 8th AJCC staging system, all oropharyngeal malignancies should be staged depending on their p16 status [@bib76]. In light of data presented and discussed here, this approach could potentially be problematic. Subsequently, sub-specific survival analysis studies in oropharynx are highly warranted.

There are recognisable limitations in this study. First of all, sincemotion controls and I bought a little motor from Orion and added it to the mount. Problem was the cluch on the motor was so tight it made moving the slow motion RA control very difficult. So I would have to use a hex wrench to unhook the motor when I did not want to use it for tracking...

Something was not working correctly on that set up. All it should take is to loosen the clutch and the gear on the worm gear shaft will 'loosen' and move independently from the shaft. Maybe the clutch was a poor match, or not aligned 'just right.' Or the shaft was bent, even a tiny bit.

Here is a problem I had that was easily fixed when I figured out what was


<?xml version="1.0" encoding="UTF-8"?>
<!-- Written by Eclipse BIRT 2.0 -->
<report xmlns="http://www.eclipse.org/birt/2005/design" version="3.2.20">
    <property name="refreshRate">30</property>
    <styles>
        <style name="My-Style">
            <property name="backgroundAttachment">scroll</property>
            <property name="backgroundColor">red</property>
            <property name="backgroundImage">file</property>
            <property name="backgroundPositionX">center</property>
            <property name="backgroundPositionY">top</property>
            <property name="backgroundRepeat">repeat</property>
            <property name="fontFamily">fantasy</property>
        Page</property>
            <property name="pageBreakAfter">auto</property>
            <property name="showIfBlank">true</property>
            <property name="canShrink">true</property>
            <list-property name="highlightRules">
                <structure>
                    <property name="operator">eq</property>
                    <property name="backgroundColor">white</property>


<expression name="mapTestExpr">[somefield]</expression>
            <list-property name="mapRules">
                <structure>
                    <property name="operator">eq</property>
                    <expression name="value1">"X"</expression>
                    <text-property name="display">Closed</text-property>
                </structure>
                <structure>
    at android.app.ActivityThread.access$1500(ActivityThread.java:123)
  04-17 18:07:54.509: E/AndroidRuntime(13783):    at android.app.ActivityThread$H.handleMessage(ActivityThread.java:939)
  04-17 18:07:54.509: E/AndroidRuntime(13783):    at android.os.Handler.dispatchMessage(Handler.java:99)
  04-17 18:07:54.509: E/AndroidRuntime(13783):    at android.os.Looper.loop(Looper.java:130)
  04-17 18:07:54.509: E/AndroidRuntime(13783):    at android.app.ActivityThread.main(ActivityThread.java:3835)

A:

Instead of getting the values through intents i have used this way to perform it...`

The class showing list of places

public void onItemClick(AdapterView<?> parent, View view,
                int position, long id) {
            // getting values from selected ListItem
          //  String name = ((TextView) view.findViewById(R.id.name)).getText().toString();


creating any database:

+

{{ic|# chattr +C /var/lib/mysql}}

+

}}

+

+

Start the {{ic|mysqld}} [[daemon]], run the setup script:

# mysql_secure_installation

# mysql_secure_installation

+

and restart the daemon afterwards.

−

Then [[Daemons#Restarting|restart]] MySQL (mysqld). If you want to start MySQL at boot time, see [[Daemons#Starting_on_boot]].

+

Frontends available are {{AUR|mysql-gui-tools}} and {{AUR|mysql-workbench}}.

{{Note|It could be needed to remove the following files from {{ic|/var/lib/mysql}} : {{ic|ib_logfile0}}, {{ic|ib_logfile1}} and {{ic|aria_log_control}} before restarting the daemon in the following procedure.}}

+

+

Users who want to switch will need to stop their current {{ic|mysqld}} daemon, install ''mariadb'', ''libmariadbclient'' or ''mariadb-clients'', restart {{ic|mysqld}}and execute:

+

# mysql_upgrade -p

+

in order to migrate their systems.

+

+

=== On update ===

+

+

You might consider running this command after you have upgraded MySQL and started it:

+

# mysql_upgrade -u root -p

== Configuration ==

== Configuration ==

+

Once you have started the MySQL server, you probably want to add a root account in order   []byte   `json:"-"`
	XXX_sizecache        int32    `json:"-"`
}

func (m *VoidProto) Reset()         { *m = VoidProto{} }
func (m *VoidProto) String() string { return proto.CompactTextString(m) }
func (*VoidProto) ProtoMessage()    {}
func (*VoidProto) Descriptor() ([]byte, []int) {
	return fileDescriptor_api_base_9d49f8792e0c1140, []int{6}
}
func (m *VoidProto) XXX_Unmarshal(b []byte) error {
	return xxx_messageInfo_VoidProto.Unmarshal(m, b)
}
func (m *VoidProto) XXX_Marshal(b []byte, deterministic bool) ([]byte, error) {
	return xxx_messageInfo_VoidProto.Marshal(b, m, deterministic)
}
func (dst *VoidProto) XXX_Merge(src proto.Message) {
	xxx_messageInfo_VoidProto.Merge(dst, src)
}
func (m *VoidProto) XXX_Size() int {
	return xxx_messageInfo_VoidProto.Size(m)
}
func (m *VoidProto) XXX_DiscardUnknown() {
	xxx_messageInfo_VoidProto.DiscardUnknown(m)
}

var xxx_messageInfo_VoidProto proto.InternalMessageInfo

func init() {
	proto.RegisterType((*StringProto)(nil), "appengine.base.StringProto")
	proto.RegisterType((*Integer32Proto)(nil), "appengine.base.Integer32Proto")
	proto.RegisterType((*Integer64Proto)(nil), "appengine.base.Integer64Proto")
	proto.RegisterType((*BoolProto)(nil), "appengine.base.BoolProto")
	proto.RegisterType((*DoubleProto)(nil), "appengine.base.DoubleProto")
	proto.RegisterType((*BytesProto)(nil), "appengine.base.BytesProto")
	proto.RegisterType((*VoidProto)(nil), "appengine.base.VoidProto")
}

func init() {
	proto.RegisterFile("google.golang.org/appengine/internal/base/api_base.proto", fileDescriptor_api_base_9d49f8792e0c1140)
}

var fileDescriptor_api_base_9d49f8792e0c1140 = []byte{
	// 199 bytes of a gzipped FileDescriptorProto
	0x1f, 0x8b, 0x08, 0x00, 0x00, 0x00, 0x00, 0x00, 0x02, 0xff, 0x6c, 0xcf, 0x3f, 0x4b,


Q:

exception propogation from jersey class

Could some one give me some pointers as to how do i propogate an exception from jersey class to lets say a JSP, along with some examples if possible..
Thanks in advance
Adhir

A:

Use a Runtime exception to propogate the exception.. WebApplicationException in the JSR311 api does the same thing for you.. if the client is a web app.
Otherwise a checked exception would suffice

Sorry, I lied. We’re going to start not with the truth, but instead with, well, the lies.

x The Economy is soooo good, perhaps the best in our countryÃ¢ÂÂs history (remember, itÃ¢ÂÂs the economy stupid!), that the Democrats are flailing & lying like CRAZY! Phony books, articles and T.V. Ã¢ÂÂhitsÃ¢ÂÂ like no other pol has had to endure-and they are losing big. Very dishonest people! — Donald J. Trump (@realDonaldTrump) September 10, 2018

Funny, but the word in that tweet that jumps out at me is the only one in all-caps. That’s the word that, according to Chief of Staff John Kelly (quoted in Bob Woodward’s new book), gave its name to the place where the White House is located—Crazytown. But I digress.

As for the only concrete statement in that


for scuba diving exist at most of the resorts in the Mamanuca and Yasawa Groups; along Viti Levu's Coral Coast and at Pacific Harbor; on small resort islands off Viti Levu; at Levuka, Nadi, and Savusavu; and on Taveuni and adjacent islands. When choosing a place to stay, pick somewhere as close as possible to the sites you wish to dive, as scuba operators generally resist spending a lot of money on fuel to commute to distant reefs.

If you've never dived before, Fiji is an excellent place to learn. Most scuba operators offer Open Water Diver certification courses lasting four or five days. For children, Subsurface Fiji at several Mamanuca resorts specializes in teaching diving to kids as young as eight! Many of the scuba operators listed inis one of the nicest low-budget places to stay in Fiji. It has 14 deluxe bure with private bath and fridge at F$210 double and three 20-bunk dorms at F$70 per person. Discounts are offered for children under 14. Mosquito nets are supplied and there's 24-hour electricity. Included in the price are three decent meals and transfers to/from the Yasawa Flyer II. Coral View's beach isn't that great, but boat trips to Blue Lagoon Beach are F$15 (minimum of five). The boat trip to the Sawa-i-Lau caves requires a minimum of five people willing to pay F$50 per person. Snorkeling gear is F$10 extra. Dive Yasawa Lagoon (www.diveyasawalagoon.com) based at Coral View charges F$110/165 for one/two tanks and gives PADI certification courses for F$600. Although there are lots


bus station.

# Health

The District Hospital (tel. 650-0455) is 1.5 kilometers southwest of Sigatoka, out on the road to Nadi.

Dr. Rudy Gerona and Dr. Aida Gerona (tel. 652-0327) work out of an office on Sigatoka Valley Road, facing the river near the old bridge. They're open weekdays 0830–1600, Saturday 0830–1300. A dentist is next door.

Patel Chemist (tel. 650-0213) is behind the market.

# Getting There and Around

Pacific Transport (tel. 650-0088) express buses leave Sigatoka for Suva at 0845, 0910, 1025, 1425, 1800, and 1945 (127 km, 3.5 hours, F$10.70), and for Nadi Airport at 0935, 1115, 1220, 1500, 1800, and 2020 (70 km, 1.5 hours, F$5.65). Sunbeam Transport (tel. 927-2113) has express buses to Suva (F$11.25) at 0640, 0800, 1050, 1130, 1220, 1320, 1500, 1645, and 1720, and to NadiAll rates include three meals of variable quality, taxes, and transfers from Vunisea Airport (three-night minimum stay). Windsurfers, kayaks, and paddleboards are free. The morning two-tank boat dive is F$180 without gear. The snorkeling off Matana's golden beach is good, and the Namalata Reef is straight out from the resort (the east end of the Great Astrolabe Reef is an hour away).

In 2000, a surfing camp called Nagigia Island Resort (tel. 603-0454, www.nagigia-island.com) opened on tiny Denham Island off Cape Washington at the west end of Kadavu. The seven little bungalows perched on a limestone cliff are F$384/715 single/double deluxe or F$358/669 single/double oceanfront. A bed in a six-bed dorm is F$268 per person. Meals and some boat trips are included, but you'll need to add 17.5 percent


offers a Pacific Bula Pass as an add-on to their tickets to Fiji from Australia, New Zealand, and the United States. For only US$99 you can fly roundtrip from Nadi to Apia, Honiara, Nuku'alofa, Tarawa, or Port Vila. To fly to all of them is US$299. This pass can only be purchased in Australia, New Zealand, and the United States prior to travel to Fiji. Only "M" class economy seats can be used, date changes cost US$50, and the pass is non-refundable.

AIR PACIFIC FLIGHT ROUTES

Obtainable locally are Air Pacific's special Bula Fares, which are based on two one-way fares at different levels, starting at F$598 round-trip to Apia, F$438 to Tongatapu, F$508 to Port Vila, F$848 to Honiara, and F$898 to Tarawa. Tax is extra. Only a fewplugs (with the two top prongs at angles). Pick up both items before you leave home, as they can be hard to find here.

# Videos

Commercial travel videotapes make nice souvenirs, but always keep in mind that there are three incompatible video formats in the world: NTSC (used in North America), PAL (used in Britain, Germany, Japan, Australia, New Zealand, and Fiji), and SECAM (used in France and Russia). Don't buy prerecorded tapes abroad unless they're the same kind used in your country. The same applies to DVDs.

# AIRPORT CODES

AKL—Auckland

APW—Apia/Faleolo

BNE—Brisbane

CHC—Christchurch

FGI—Apia/Fagalii

FUN—Funafuti

HIR—Honiara

HNL—Honolulu

ICI—Cicia

INU—Nauru

IUE—Niue

KDV—Kadavu

KXF—Koro

LAX—Los Angeles

LBS—Labasa

LEV—Levuka

LKB—Lakeba

MEL—Melbourne

MFJ—Moala

MNF—Mana

NAN—Nadi

NGI—Gau

NOU—Nouméa

OSA—Osaka

POM—Port Moresby

PPG—Pago Pago

PPT—Papeete

PTF—Malololailai

RAR—Rarotonga

RTA—Rotuma

SEA—Seattle

SFO—San Francisco

SIN—Singapore

SUV—Suva

SVU—Savusavu

SYD—Sydney

TBU—Tongatapu

TRW—Tarawa

TVU—Taveuni

TYO—Tokyo

VBV—Vanua Balavu

VLI—Port Vila

WLG—Wellington

WLS—Wallis

YVR—Vancouver

YYZ—Toronto

# MARITIME COORDINATES

# DIPLOMATIC OFFICES

Permanent Mission to the United Nations: 801 Second Ave., 10th Floor, New York, NY 10017, USA; tel. 212/687-4130, www.fijiprun.org

Embassy of Fiji: 2000 M Street NW, Suite 710, Washington, DC 20036,


roti becomes naan, a Punjabi specialty similar to pita bread. Papadam is a crispy version of the same. Palau is a main plate of rice and vegetables always including peas. Samosas are lumps of potato and other vegetables wrapped in dough and deep-fried. Pakoras are deep-fried chunks of dough spiced with chili and often served with a pickle chutney. A set meal consisting of dhal, roti, rice, one or two curries, and chutney, served on a metal plate, is called a thali. If meat is included, it's called simply a non-vegetarian thali. Yogurt mixed with water makes a refreshing drink called lassi. If you have the chance, try South Indian vegetarian dishes like iddili (little white rice cakes served with dhal) and masala dosa (a potato-filled rice pancakeJames Ford (One Life to Live)

James Ford is a fictional character from the ABC original soap opera One Life to Live. The role was played by Nic Robuck from June 9, 2010, to January 11, 2012.

Casting and character creation
Actor Nic Robuck reveals in an interview with ABC Daytime that his pairing with Starr (Kristen Alderson) is a big hit with fans, "We have fans that wait outside the studio every day with pictures, and they talk about the storyline. They always ask, 'Are you and Starr going to get together?' I always tell them, 'You'll just have to wait and see." Robuck also describes his character James,-ABC Daytime

Originally, Robuck was just supposed to be brought as Starr's new love interest, but when the writers noticed the resemblance


equine, but not canine, MaSC {#s2_2}
----------------------------------------------------------------------

Despite the similarities in DMBA metabolism and DMBA-induced DNA damage observed in equine and canine MaSC, a striking difference was noted in the phenotype of these cells in relation to their ability to remain viable after DMBA treatment. In essence, we found that treatment with 5 µM DMBA significantly reduced the viability of EqMaSC, but not CaMaSC (Figure [2A](#F2){ref-type="fig"}). Specifically, a significant reduction in cell metabolism was observed, using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Figure [2A(i)](#F2){ref-type="fig"}), and a significant increase in lactate dehydrogenase (LDH), a cytosolic enzyme that is an indicator of cellular toxicity, was observed, using an LDH release assay (Figure [2A(ii)](#F2){ref-type="fig"}), in DMBA-treated EqMaSC. In contrast, no effect on cell metabolism or LDH release was observed in DMBA-treated CaMaSC (Figure [2A](#F2){ref-type="fig"}).

![Equinesignificantly less by 48 h and steadily decreased further over time (Figure [2E](#F2){ref-type="fig"}). These results indicate that CaMaSC indeed repair their DMBA-induced DNA damage, or alternatively, that there are overall less, but viable, cells in the population over time.

DMBA treatment induces transcriptional changes in equine, but not canine, MaSC {#s2_3}
------------------------------------------------------------------------------

To better characterize the effects of DMBA on MaSC from equine and canine origin on a molecular level, we decided to perform genome-wide transcriptome profiling. To this end, MaSC samples from three individual animals per species were treated with either vehicle (DMSO) or DMBA for 4 h and submitted for RNA deep sequencing. The 4 h treatment was selected to capture a time point before any changes in MaSC viability from either species were observed (data not shown). In


on the selected composition of vortices. [Figure 3(c)](#f3){ref-type="fig"} shows mean current density given by , where the mean is surface-averaged and time-averaged over a long time after the initial transition. Here each symbol (circles in [Fig. 3(c)](#f3){ref-type="fig"}) at a given voltage represents a single simulation with only the small random initial perturbation changed between simulations. The shaded region represents an approximate range of possible mean current densities, while the upper and lower bounds, indicated by the dashed lines, are extracted from conditional means over zones of large and small vortices respectively. Details of the calculation of these values as well as vortex size distribution statistics are provided in the [supplementary information](#S1){ref-type="supplementary-material"}.

The large vortices drive much larger currents, by a factor of approximately 2, via two mechanisms. One isvoltages, one vortex pair per pattern period is generated ([Fig. 6(a)](#f6){ref-type="fig"}). These vortices are driven at the intersections between permeable and impermeable regions with strong outflow over the membrane and inflow over the impermeable zone. They provide additional advective transport, even at voltages lower than the critical voltage, thereby preventing a plateau at the traditional limiting current. Instead, a new type of plateau occurs between and due to changes in the vortex structure.

As can be seen in [Fig. 6(b)](#f6){ref-type="fig"} and [Supplementary Movie S5](#S1){ref-type="supplementary-material"}, at Δ*V* = 20*V*~*T*~ a second pair of small counter-rotating vortices develops on each membrane region. These secondary vortices grow in size with increasing applied potential ([Fig. 6(c)](#f6){ref-type="fig"}) while the initial vortices shrink. This shrinkage of the large scales reduces the effectiveness of the system for transporting


---
abstract: 'We present evidence that the globular cluster NGC 6397 contains two distinct classes of centrally-concentrated UV-bright stars. Color-magnitude diagrams constructed from , , , and i data obtained with the /WFPC2 reveal seven UV-bright stars fainter than the main-sequence turnoff, three of which had previously been identified as cataclysmic variables (CVs). Lightcurves of these stars show the characteristic “flicker” of CVs, as well as longer-term variability. A fourth star is identified as a CV candidate on the basis of its variability and UV excess. Three additional UV-bright stars show no photometric variability and have broad-band colors characteristic of B stars. These non-flickering UV stars are too faint to be extended horizontal branch stars. We suggest that they could be low-mass helium white dwarfs, formed when the evolution ofa red giant is interrupted, due either to Roche-lobe overflow onto a binary companion, or to envelope ejection following a common-envelope phase in a tidal-capture binary. Alternatively, they could be very-low-mass core-He-burning stars. Both the CVs and the new class of faint UV stars are strongly concentrated toward the cluster center, to the extent that mass segregation from 2-body relaxation alone may be unable to explain their distribution.'
author:
- 'Adrienne M. Cool'
- 'Jonathan E. Grindlay'
- 'Haldan N. Cohn and Phyllis M. Lugger'
- 'Charles D. Bailyn'
title: 'Cataclysmic Variables and a New Class of Faint UV Stars in the Globular Cluster NGC 6397[^1]'
---

-1[yr$^{-1}$]{} $U_{336}$ $V_{555}$ i[$I_{814}$]{} \#1[\_[[\#1]{}]{}]{}

=cmbx12 =cmti8 =cmr8

Introduction
============

The center of NGC 6397 is a prime region in which to investigate the effects of stellar interactions. At just 2.2 kpc, its high-density core


by which this might be accomplished are: (1) Roche-lobe overflow onto a binary companion (Webbink 1975); (2) a collision that produces a common-envelope system, followed by envelope ejection (Davies, Benz, & Hills 1991). In either case, the formation rate is necessarily linked to post–main-sequence evolution. An order of magnitude estimate of the fraction of RGB stars that would need to be stripped can be made by noting that there are six horizontal branch (HB) stars within the PC1 chip where the three NFs reside. While the lifetimes of He WDs are uncertain (see, , Iben & Tutukov 1986), if we assume that they are similar to those of HB stars (10$^8$ yrs), then $1 \over 3$ of all RGB stars would need to be stripped. If binaries alone were responsibleit was useful in older games when rendering without proper gamma correction. The display gamma plus an inverse square falloff would combine to make the light falloff look too sharp. By using an inverse linear falloff (a ~ 1, b ~ 0) we can cheat it to look more correct, letting the display gamma do the squaring. In modern games though, we'd prefer to do proper gamma correction (in addition to things like HDR tonemapping) to make sure our colours look right, so nowadays we'd usually only play with these coefficients for stylistic reasons.
tl;dr: a = 0, b = 1 is a pretty safe bet unless you want a more stylistic effect.


2. Flushes: You need to have flushes in the back hand - middle hand and have your top front hand in the same suit.

Instant win and player wins 3 from everyone and voids all other hands.

      3. Six & Half Pairs: You need to have six pairs in your hand. The last card can make three of a kind.

Instant win and player wins 3 from everyone and voids all other hands.

The Justice Department has proposed a new domestic spying measure that would make it easier for state and local police to collect intelligence about Americans, share the sensitive data with federal agencies and retain it for at least 10 years.

The proposed changes would revise the federal government's rules for police intelligence-gathering for the first time since 1993 and would apply to any of the nation's 18,000 state and local police agencies that receive roughly $1.6 billion each year in federal grants.

Quietly unveiled late last month, the proposal is part of a flurry of domestic intelligence changes issued and planned by the Bush administration in its waning months. They include a recent executive order that guides the reorganization of federal spy agencies and a pending Justice Department overhaul of


The wards are paid for when they are shipped, so the money isn’t lining pockets of government officials or funding missile programmes.

Linton said everything was going well until December. A pilot project with 10 wards is operating on the outskirts of Pyongyang.

“Then we ran into this sanctions problem,” he said. “It’s not that we can’t send anything in. But these buildings have a metal roof – a panel that has metal in it, aluminum – and that’s the issue.”

Linton said Eugene Bell has $250,000 of prefabricated wards rotting in a dockyard outside Seoul. As an American, he is concerned the US will be held responsible for whatever happens with tuberculosis in North Korea.

“Unless something is done and done soon, this medical emergency will become known in Korea asas outlined here
and here.)

Guttural
came to be used as a descriptor not just for consonants in Hebrew and
other Semitic languages like Arabic, but also for some sounds in
European languages, such as the voiceless velar
fricative /x/ in German Bach,
Dutch van Gogh, and
Scottish loch. Of course,
English has consonants with velar articulation
(the stops /k/ and /g/ and the nasal /ŋ/), not to mention a glottal
fricative (/h/), but guttural
has been inexactly associated with foreign consonants that sound
"throaty" to English speakers. With the advent of modern articulatory
phonetics, the term has largely dropped out of use among linguists (though
it still retains some currency in studies of Hebrew).

The folk-linguistic sense lives on, however, in English speakers'
impressionistic portrayals of the perceived "harshness" of languages
like German or Arabic, or even of other English dialects. In
contemporary usage it's one


and guttural. When Howard Dean emitted his famous scream in his concession speech after the 2004 Iowa caucuses, it was often described at the time as "guttural." Did that mean the scream was throaty, vulgar, or visceral? For many observers, it was all three at once. Now that's guttural politics.fee to the delivery guy?? How much would the fee be if ever for 3 figures? When the filmation sub kicks in, I'll end up with at least 3 figures a month!!!!

i think DHL brokerage fee is 7.95 + whatever the GST or HST on the invoice total.

my god... there is no break on buying more then one figure.. for every additional figure you get they tack on another 8-10 bucks..
1 figure is 13.75 then it's 8 and change for each additional figure so 4 figures in one medium size box is 37 bucks.. which weights about 4 pounds.. and i'm being generous. i just ordered Castle Grayskull - the statue- from BBTS which is weighed in at just under 7 pounds and it was 23 bucks USPS


air mail 5-8 days delivery.. Tell me Matty and DR are not rolling us over... and each time Scott says they make no money on shipping.. my blood boils..

yep. it's why I let my sub die. Spending $150 one figures I don't want doesn't make sense to me. I'd rather buy someone's extra give them a bit of a profit than deal with the RIDICULOUS shipping and Scott's two faced tactics.

well just a check.. for 4 cases of the zolo style cases.. which weight pretty nothing to ship to the US is 13.75

can some one verify what it would be for international, and regardless of country it's the same.

Ie i just did a deal with a member from Denmark and his shipping for FFM and Kar was thepublic auction, there is only a few information on the other forms of transaction, hence leaving the total size of the art market remains unexplored. The price of a painting is also unpredictable. For instance, Bach and Vuong (2015) told the story of a painting Daniel in the Lions' den by Peter Paul Rubens, which was first purchased by Christie's London in 1882 for US$2,292. However, the art was doubted to be the work of Jakob Jordaens which put its price at only US$682 in 1963. Two years later, there was evidence to authenticate that Rubens is the author of the painting and its price shot up to US$243,755. The Vietnamese art and Vietnamese art market are minors in comparison with the international counterparts, but as Vietnam is