preprint. arXiv:1401.3181****

K. A. Kirkpatrick, *Uniqueness of a convex sum of products of projectors, J. Math. Phys. [**43**]{} (2002), 684–686.*

S.-H. Kye, *Facial structures for various notions of positivity and applications to the theory of entanglement, Rev. Math. Phys. **25 (2013), 1330002. S.-H. Kye, *Faces for two qubit separable states and the convex hulls of trigonometric moment curves, Probab. Math. Statist. [**33**]{} (2013), 385–400. S.-H. Kye and H. Osaka, *Classification of bi-qutrit positive partial transpose entangled edge states by their ranks, J. Math. Phys. [**53**]{} (2012), 052201. A. Peres, *Separability Criterion for Density Matrices, Phys. Rev. Lett. **77 (1996), 1413–1415.********

Ł. Skowronek, *Three-by-three bound entanglement with general unextendible product bases, J. Math. Phys. **52 (2011), 122202. J. Tura, R. Augusiak, P. Hyllus, M. Ku' s, J. Samsonowicz and M. Lewenstein, *Four-qubit{
  "Uuid": "f20adbc6-41e9-4118-a55c-70b2ff8861fb",
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      "Code": "import clr\r\nclr.AddReference('RevitAPI')\r\nfrom Autodesk.Revit.DB import *\r\n\r\nclr.AddReference(\"RevitNodes\")\r\nimport Revit\r\nclr.ImportExtensions(Revit.Elements)\r\n\r\nclr.AddReference(\"RevitServices\")\r\nimport RevitServices\r\nfrom RevitServices.Persistence import DocumentManager\r\nfrom RevitServices.Transactions import TransactionManager\r\n\r\ndoc = DocumentManager.Instance.CurrentDBDocument\r\ncompvol = IN[0]\r\ncompvolstate = IN[1]\r\n\r\nif compvol != compvolstate:\r\n\tTransactionManager.Instance.EnsureInTransaction(doc)\r\n\ttry:\r\n\t\tAreaVolumeSettings.GetAreaVolumeSettings(doc).ComputeVolumes = compvol\r\n\t\tOUT = True\r\n\texcept: OUT = False\r\n\tTransactionManager.Instance.TransactionTaskDone()\r\nelse: OUT = True",
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dimethyl disulfide and dimethyltrisulfide coinciding with strongly enhanced removal of methanethiol was observed for the untreated filter. This was assumed to be enhanced by an incidence of low local air velocity in the filter and indicated involvement of iron-catalyzed reactions in the removal of sulfur compounds.proof the previous partnerships have finished, corresponding to by offering divorce documentation or a death qualification. Some Thailänder registration places of work likewise require given of Australian residents being licensed by Embassy. There exists a charge for this service, so that you could which in turn to examine with the enrollment office which you plan to use for determine if that’s needed is by their work environment.

Polygamy certainly is the act or condition of a person getting married to another individual whereas still being lawfully married to a new partner. The crime is https://legitmailorderbride.com/asian-brides/ punishable with a fine, imprisonment, or each, in accordance with the law individuals state and the circumstances from the offense.


the California recycling program, the $450 million state buy-back financed by all those nickel deposits on bottles and cans. The program was launched for environmental reasons, but to him it presented an opportunity for graft and larceny.

In 1999, he followed that same path to Pets R Us. Sarkis Antonyan bought the place early that summer, along with two other small storefront pet shops, one in Glendale and another in Tujunga. He never showed much interest in small-animal husbandry; according to Burbank police, he was using the stores as fronts to carry out credit-card fraud.

It was September 14, 1999, and the place was a mess at the end of a long, sweaty day. The bird cages stank, and the water in the fish tanks was dingy, but Antonyan didntby convening a task force. When we realized we were dealing with a fairly organized group of individuals, we felt it would be of interest to other law-enforcement agencies, he said.

Fawrup declined to specifically name the FBI, and a spokesman for the bureau refused to comment, but other sources confirm that the bureau opened an a investigation as early as 1998. The FBI started its investigation because recycling people went to them, said Joe Massey, of the Independent Recyclers. Massey said legitimate operators welcomed the inquiry. We would love to get anyone who is committing fraud out of the program.

The prospect of federal gumshoes prompted excitement throughout the industry, but when no indictments were issued and no public action was taken, that excitement turned to annoyance. The frustration


a team that committed a string of takeover robberies in the jewelry district and elsewhere. In three of those crimes, witnesses said Darbinyan was armed and, on two occasions, pistol-whipped victims who failed to cooperate promptly. On another occasion, a store owner said he opened fire on Darbinyan and his cohorts during the course of a robbery; They returned fire before leaving with a van full of loot. Police sources say Darbinyan also on occasion drafted members of the Temple Street Gang to act as sluggers, a surprising mix of underworld muscle that investigators say serves as a measure of his clout.

When Darbinyan turned up as a suspect in one of those robberies, it triggered police to look again at the Hi-Tech Jewelry heist. Detective Speer promptly contactedthe new world of immigrant Los Angeles. My husband had a heart attack. My son is dead. Everyone knows, but nobody can touch it.

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b(k) = -28*k - 1258. Let q be b(-45). Let h(a) be the second derivative of -2*a**q + 0 - 5/3*a**4 + 4*a**3 - 26*a. Factor h(m).
-4*(m - 1)*(5*m - 1)
Let g(b) = -373*b**2 + 7*b + 11 + 12 + 384*b**2 + 7. Let n be (-1)/(3 - (-101)/(-34)). Let p(j) = 2*j**2 + j + 5. Let u(h) = n*p(h) + 6*g(h). Factor u(t).
-2*(t - 5)*(t + 1)
Let l(n) = -n**2 - n - 1. Let s(m) = -m**3 + 6*m**2 + 8*m - 13. Let f(w) = -5*l(w) + s(w). Let v be f(12). Solve 2 - 6 - 4*o**v + 6*o**2 + 2*o**2 = 0.
-1, 1
Let s(u) = 7*u**3 + 53*u**2 + 218*u + 172. Let t(g) = -6*g**3 - 54*g**2 - 219*g -many attempts to develop an effective one. Although encouraging findings came from experiments in various animal models, human studies have been disappointing, unfortunately. The vaccine strategies that have undergone clinical evaluation until today included autoinoculation of live HSV, whole inactivated vaccines, attenuated live virus vaccines, modified live virus subunit vaccines, cell culture-derived subunit vaccines, recombinant subunit (glycoprotein) vaccines, DISC (Disabled Infectious Single Cycle) virus vaccines, viral vectors and naked DNA vaccines. In most of the clinical studies the failure of HSV vaccines in spite of inducing very high levels of specific neutralizing antibodies have emphasized that cell-mediated immune response, especially Thl type immunity is important in preventing both primary disease and recurrences with HSV, rather than humoral response. The most hopeful result was obtained with HSV-2 gD and


cavorting with the Misfits and performing with a punk band called Satan’s Cheerleaders during the 1980s when she was in her sixties. At one point Nurmi got into some legal disputes stemming from the rights to Vampira’s image including one lawsuit Nurmi launched against Cassandra “Elvira” Peterson for ripping off her Vampira image, which was dismissed. Despite this, Nurmi’s “Vampira” character continues to endure since she conceived of her over 60 years ago. She was played by Lisa Marie in Tim Burton’s film, Ed Wood.

Somewhat rather underappreciated during her time, Maila Nurmi was lovingly profiled in the 2012 documentary Vampira and Me which featured newly restored kinoscopes of her TV appaearances. Some of the photos that follow (though tame) might be slightly NSFW because, bikinis.

Charles Addams cartoon drawn"Listen, it sounded like the brown-breasted bottle-beaked hooting owl." "Sherman, did you really bring me all the way out here just to look at birds?" "I thought you liked birds." "Yes, but sometimes there are other things I'd like to see." "Like what?" "Like a movie." "Shh." "Hear that?" "The owl is near." "Sherman." "Yes?" "Does it have unusually large eyes?" "Oh, yes." "I'd say so." "It's a nocturnal predator." "And a giant pointy beak and fur?" "You know, Treena, I'd be up for a movie." "He did it." "Scooby-Doo captured the monster." "Scooby-Dooby-Doo." "It's not a real monster." "It's actually Miss Thelmer, the company accountant." "My plan was perfect." "How did you ever...?" "Hi, Mom." "Can't talk, exposition time." "What?" "Hang on." "Will you take the wrap-up,


is Whirlen Merlin when he was onstage." "Isn't he awesome?" "I had no idea you were so into magic, Madelyn." "At least you've finally settled on something." "Where were you last semester, clown college?" "Heh, heh." "Yeah, so I've done some soul searching." "Not everyone is born with a mystery book in her hand, Velma." "Well, I think magic is really cool." "I've always been fascinated by how it's done." "Just as Velma loves to solve mysteries I love to uncover the secrets of magic, and this is the perfect school for that." "What about that griffin?" "Mom said..." "Mom's just dramatic." "It's a silly old myth." "That's our dinner bell." "Alakazam" "And because you're our special guests of honour I've asked the chef..." "That's me." "To prepareof nowhere?" "Not really." "I was watching Crystal." "All she did was point." "It's the way she did it so effortlessly." "I can point too." "Usually when you do you knock my glasses off." "Easy, Velma." "She hasn't done that in at least an hour." "Ha, ha." "Very funny, scarf boy." "Griffin!" "It's the griffin!" "Oh, my gosh, there it is." "That's right, Daddy." "I told you it was real." "Holy Houdini." "Open sesame, open sesame" "Open sesame now" "This way, guys." "When I enrolled Maxwell in your school I didn't expect dodging monsters to be a part of the curriculum." "Good day, sir." "But we didn't know the griffin was real." "Poor Whirlen." "There go the rest of his students." "Where are you going?" "Aren't we taking


how you saved us like that." "Yeah, well, I do have some major feet." "It's more than your feet, Shaggy." "It's you." "You're so amazing, so forceful." "I think you're the bravest, most wonderful boy I have ever known." "Oh, gee, Madelyn." "I like you too." "Aw." "Mwah, mwah, mwah." "It's getting late." "What are you waiting for?" "Directions?" "Drive!" "Shoo, griffin." "Like, be gone already." "I think the batteries are dead." "Hey, I put a box of magic explosives in the van for practice on the field." "Like, ScoobyDoo, let's strike this griffin out." "Yeah, strikeout." "Uhoh." "I think Scooby dropped a live explosive in the box." "Like, which one?" "I can't tell." "We haven't lost him yet, guys." "Hurry, inside." "Hey, you're going to leave mewith the glasses to put us down." "Like, dude, we're losing altitude." "Little help, little help!" "Shaggy got him." "Isn't that incredible?" "Yeah." "You really are brave." "Like, don't squeeze me, Madelyn." "You're denting my metal." "The poor thing looks hurt." "Why doesn't it fly away?" "There's your answer." "The griffin, it's a gigantic puppet?" "Correct." "This whole charade is making sense now." "Run!" "Whoa." "Marlon, you?" "Marlon operated that contraption trying to scare everyone away once and for all." "But Shaggy and Scooby spoiled his plans." "So the monster never was real." "No, just a more sophisticated version of the puppets in your magic act when you and Marlon were kids." "The blimp carried the griffin beneath it to make it look like it was flying." "But


how did he disguise the blimp?" "Remember how every time the griffin appeared, it was foggy?" "The blimp generated its own concealing fog bank." "I noticed some of the bottles in Marlon's workshop were marked glycerol." "A chemical used by professional illusionists in fog machines." "I used the fog to hide the blimp then worked the griffin through this handheld animatronics control." "What about when the griffin attacked us in the tower?" "It couldn't have been the same puppet." "It wasn't." "After Whirlen bought the castle, I started renovating the Griffin's Roost." "I discovered Lord O' Flannery had rigged parts of the tower with devices of his own to make superstitious people think a monster lived there." "When trespassers entered the tower they would be driven away byLove for divorced dads: four dating sites and particularly for divorced parents, diving back into the dating world four dating sites worth checking out. Grigiskes's best 100% free divorced singles dating site meet thousands of divorced singles in grigiskes with mingle2's free divorced singles personal ads and chat rooms our network of single men and women in grigiskes is the perfect place to make friends or find a boyfriend or girlfriend in grigiskes. If you're dating a divorced man, here are some tips from relationship experts on what you need to know as your relationship moves forward. Matchcom, the leading online dating resource for singles search through thousands of personals and photos go ahead, it's free to look.

Watch video divorce is one of the most traumatic events we


Q:

Slider with vertical parallax effect

I've created a simple slider for my client to be able to update using Flexslider, and elsewhere on their site they have a vertical scrolling parallax effect. Now they want a slider with vertical scrolling parallax effects...
Here's an example of the desired effect
For my parallax effect I used Parallax.js, which was great when I only needed a single image to have the effect, but not much use now I need the slider to have it.
The above example uses Avia Slider, but the parallax effect appears to be done by something else (as Avia doesn't have any such effect that I can see).
How can I create a simple parallax effect for my existing Flexslider slider (or any other slider)? It seems like it should bemuch an intentional insult, isn’t it?

Anyway, this got me curious. As near as I can tell, the Dutch king and queen met with Putin on Friday, the day before the pageant. On the day of the pageant itself, the royals visited an art gallery; met with Dutch and Russian CEOs to discuss “strengthening trade between the two countries”; and then attended an evening concert, where a pair of activists pelted them with tomatoes. I can find no evidence that they met with Putin that day at all, let alone that a traffic jam held them up. So not only was Putin insulting Trump with a phony excuse, he made sure it was an excuse that was really obviously phony unless you’re an idiot.


gases both inside and outside the country. Air pollution has reached hazardous level in many parts of

Approximately 45 national and international experts on the Upper Indus Basin and media persons will observe glacier monitoring stations, hydrological stations, and weather stations installed by the Pakistan Meteorological Department, Water and PowerExpress Preemption
We first observe that there is no express preemption provision in the FDCA regarding drugs. "Drug" is defined in relevant part as "articles intended for use in the diagnosis, cure, mitigation, treatment, or prevention of disease in man or other animals ... [and] articles (other than food) intended to affect the structure or any function of the body of man or other animals[.]" 21 U.S.C. § 321(g)(1). Acetaminophen plus codeine is a drug. Nevertheless, Purepac points to an express preemption provision in the FDCA, specifically 21 U.S.C. § 360k(a), but that provision is located in the Medical Device Amendments of 1976(MDA) portion of the FDCA. That provision provides:
[N]o State or political subdivision of a State may establish or continue in effect with respect to a device intended


similar questions. In so doing, the arguments on both sides have been ably laid out and need not be rehashed here at length. Thus, I write briefly to express and explain my view that the majority has come down on the wrong side of the question, and that applicable FDA regulations have indeed preempted Indiana common law with respect to the content of warning labels on drugs.
The majority dismisses as precedent any cases concerning the Medical Devices Amendments of 1976(MDA) "[b]ecause drugs and devices are defined and treated differently," Slip op. at 8, and because the MDA contains an express preemption provision, whereas the Federal Food, Drug, and Cosmetic Act (FDCA) does not. The lack of a preemption clause is by no means conclusive proof that preemption was470, 116 S.Ct. 2240, 135 L.Ed.2d 700 (1996), the United States Supreme Court determined that, with respect to drug package labeling, duties arising solely under state common lawand not arising under the *857 MDAconstitute requirements different from, or in addition to, FDA requirements. In the face of an MDA provision forbidding a state from establishing requirements that differ from those established in the MDA, the Court concluded that state common law was preempted in that area. Although we are concerned in the instant case with the FDCA and not the MDA, the principle in Medtronic applies here as well. State common law may not impose requirements different from or in addition to requirements set out in the FDCA concerning labeling, because Congress has expressed its intent to preempt


711 series

The  was an AC electric multiple unit (EMU) train type operated by Hokkaido Railway Company (JR Hokkaido) on Sapporo area suburban services in Hokkaido, Japan, from 1968 until March 2015. It was the first AC EMU train to be operated in Japan by the former Japanese National Railways (JNR). The last remaining trains in service were withdrawn on 13 March 2015.

Fleet
By 1 October 2014, 36 vehicles (12 sets) were still in service, all based at Sapporo Depot.

Formations
The 711 series trains were formed as three-car sets with one motored intermediate car and two non-powered driving trailer cars, as shown below.

Car 2 was fitted with one N-PS785 single-arm pantograph.

Former prototype sets S901/902
The former prototype sets, S901 and S902 were formed as shown below.

Interior
Seating consisted of a mixture ofContemplating revenge, Sporting must control their emotions to beat Houston

Stitch together the slow-motion montage. Cue the epic music. Bring back the late Don LaFontaine to intone: “And this time … it's personal.”

To be sure, Sporting Kansas City haven't forgotten their 2-0 home loss to Houston in last year's MLS Eastern Conference final.

“Nobody forgot about last year,” right back Chance Myers said on Friday. “I know the fans didn't, and I know we didn't. So it fuels our fire, I guess.”

They've drawn on that bitter defeat for motivation all through the 2012 campaign, and turned that focus into a U.S. Open Cup title and a second straight finish atop the East standings.

“After the Houston game last year, that was one of the worst feelings I've ever had in my


the intervention, itself \[[@B45]\]. The goal of the current study was to examine a novel approach for delivering parent-implemented intervention in families\' homes and supporting parent-child learning.

Telehealth uses communication technologies to deliver specialized services in real time over a geographical distance \[[@B10], [@B49]\]. It can be accessed at any time of day, in any location with basic, inexpensive equipment, and uses interactive, personalized features to communicate and share information \[[@B2]\]. Telehealth such as computerized software programs, videoconferencing, and virtual 3D interactive programs has been used to teach various communicative, social, emotional, and academic skills to older children and adolescents with ASD (see \[[@B4], [@B54]\] for reviews). However, the majority of this training has occurred in simulated environments \[[@B32]\], classroom settings \[[@B14], [@B27]\] or through online distance learning programsas communicative strategies to deliver and receive information effectively, interpersonal skills to develop the rapport and trust with families, and methods for protecting families\' rights to privacy via the Internet \[[@B31]--[@B44]\]. The current study provided a framework for developing and testing a telehealth approach to service families; however, the preliminary nature of the findings makes it impossible to confirm its efficacy let alone its effectiveness in comparison with other community practices. Additional research is currently underway in our center to continue testing and strengthening our approach to telehealth-delivered parent intervention with the goal of disseminating a defined parent training curriculum to community early intervention programs.

Recent trends with large, randomized and methodologically sound parent-implemented studies note strong effects for assessor-rated parent-child interactions and yet such gains have not produced


N=2 Maxwell-Einstein Supergravity and Jordan Algebras,” Nucl. Phys. B [**242**]{} (1984) 244. [[DOI:[10.1016/0550-3213(84)90142-1](http://dx.doi.org/10.1016/0550-3213(84)90142-1)]{}]{} M. Gunaydin, G. Sierra and P. K. Townsend, “Exceptional Supergravity Theories and the MAGIC Square,” Phys. Lett.  [**133B**]{} (1983) 72. [[DOI:[10.1016/0370-2693(83)90108-9](http://dx.doi.org/10.1016/0370-2693(83)90108-9)]{}]{} M. Gunaydin, L. J. Romans and N. P. Warner, “Compact and Noncompact Gauged Supergravity Theories in Five-Dimensions,” Nucl. Phys. B [**272**]{} (1986) 598. [[DOI:[10.1016/0550-3213(86)90237-3](http://dx.doi.org/10.1016/0550-3213(86)90237-3)]{}]{} M. Gunaydin, G. Sierra and P. K. Townsend, “Gauging the d = 5 Maxwell-Einstein Supergravity Theories: More on Jordan Algebras,” Nucl. Phys. B [**253**]{} (1985) 573. [[DOI:[10.1016/0550-3213(85)90547-4](http://dx.doi.org/10.1016/0550-3213(85)90547-4)]{}]{} L. J. Romans, Nucl. Phys. B [**267**]{} (1986) 433. doi:10.1016/0550-3213(86)90398-6 H. Lu, C. N. Pope and T. A. Tran, “Five-dimensional N=4, SU(2) x U(1) gauged supergravity from type IIB,” Phys. Lett. B [**475**]{} (2000) 261. [[DOI:[10.1016/S0370-2693(00)00073-3](http://dx.doi.org/10.1016/S0370-2693(00)00073-3)]{}]{} \[[[[hep-th/9909203](http://www.arXiv.org/abs/hep-th/9909203)]{}]{}\]. S. Chimento, T. Ortín and A. Ruipérez, “Yang-Mills instantons in Kaehler spaces with one holomorphic isometry,” [[arXiv:[1710.00764](http://www.arXiv.org/abs/1710.00764)]{}]{} \[hep-th\]. To be published in Physics Letters B. T. Ortín and C. Santoli, “Supersymmetric solutions of SU(2)-Fayet-Iliopoulos-gauged $\mathcal{N} = 2, d = 4$ supergravity,” Nucl. Phys. Bis. If this is greater than or equal to m_alert_pointers.size()
			// it means we need to request new alerts from the main thread.
			mutable int m_alert_pointer_pos = 0;
#endif

			// handles disk io requests asynchronously
			// peers have pointers into the disk buffer
			// pool, and must be destructed before this
			// object. The disk thread relies on the file
			// pool object, and must be destructed before
			// m_files. The disk io thread posts completion
			// events to the io service, and needs to be
			// constructed after it.
			disk_io_thread m_disk_thread;

			// the bandwidth manager is responsible for
			// handing out bandwidth to connections that
			// asks for it, it can also throttle the
			// rate.
			bandwidth_manager m_download_rate;
			bandwidth_manager m_upload_rate;

			// the peer class that all peers belong to by default
			peer_class_t m_global_class{0};

			// the peer class all TCP peers belong to by default
			// all tcp peer connections


Pages

Sunday, October 25, 2015

And to all a Good Night

Hello Friends! I finally decided that I couldn't wait for time to become available, and I would just make some! I decided to get my fingers all covered in ink this weekend...and that's exactly what happened. Here is my first card in a long time, and I decided to make it a Christmas card. Yes, it's only October, and I somehow skipped over Thanksgiving, but I will come back to that later. Promise!

Here is what I came up with! And today, I am also going to include a photo tutorial.

And here is how I created it:

Step 1:
On a 3.75" x 5" piece of Whisper White cardstock, using your sponge, lightly sponge on Soft Sky ink. Generally, I do this byWonderland Stamp Set and Archival Black ink, it's time to stamp the trees in the foreground. These will be the darkest color and you will NOT want to stamp off your ink first. Place the trees in varying heights for more interest.

Step 7:
To give the illusion of depth it's now time to add the trees in the background. Do this by inking your stamp and stamping off onto your scratch paper one time. Randomly stamp in the gaps in between your foreground trees. You should be able to get 2-3 trees from one inking.

Step 8:

Now you can remove the post it moon from your cardstock. Using the sponge, lightly add some shadows (or hazy clouds) over your moon. Nice effect, isn't it?

Step 9:

Using Santa and his reindeer stamp


open source project software designed for Scrum.
Manage your Agile developments with a virtual task board and sticky notes using open source project software designed for Scrum.

da z0 Gravity

z0 Gravity is a comprehensive project management solution that allows you to easily monitor and track all your innovation projects. We offer an inclusive and custom designed project management solution with a 360 view of your business activity. Federate your employees around a common goal, track your progress in real time and share your key information with your coworkers. We host our data in France and Germany.
Project and portfolio management solution tailored to your specific needs to streamline collaboration and work coordination.amino acids, the symmetric covariance matrix $C$ has linear size equal to $3N$.

It is important to notice that the matrix element of eq. \[eqn:c\] can be equivalently rewritten as: $$C_{ij,\mu\nu} = \sum_{l=1}^{3N} \lambda_l \, v^l_{i,\mu} v^l_{j,\nu}
\label{eqn:spectr}$$

where $\lambda_1,\, \lambda_2,...$ are the eigenvalues of $C$ ranked by decreasing magnitude and ${\bf v}^1,\, {\bf v}^2,...$ are the corresponding orthonormal eigenvectors.

Because the protein overall mean square fluctuation is given by $$\sum_{i,\mu} \langle \delta r_{i,\mu}(t)^2 \rangle = \sum_{i,\mu} C_{ii,\mu\mu}= \sum_l \lambda_l$$ one has that top ranking eigenvectors of $C$ embody the independent degrees of freedom that most contribute to the internal dynamics of the protein. Indeed, for most globular proteins of 100-200 amino acids, the top 10 eigenvectors suffice to capture most of the protein mean square fluctuation[@garcia92]. For this reason, considerations are typically


model, which employs two centroids per amino acids (for main- and side-chain, respectively). The linear correlation coefficient of the $\sim$20,000 corresponding distinct entries of the two matrices was significant (equal to 0.8) like the consistency of the two sets of essential dynamical spaces. [A more recent example of the good accord of protein structural fluctuations computed with elastic network models and MD atomistic simulations is provided by the work of Romo and Grossfield on GPCRs membrane proteins[@Romo:2011:Proteins:20872850]. This study showed that a suitably-parametrized model can match the essential dynamical spaces and their relative weight observed in microsecond-long simulations[@Romo:2011:Proteins:20872850].]{}

This agreement is noteworthy in consideration of the highly complex free energy landscape explored by folded proteins can explore in thermal equilibrium. In fact, this landscape presents several tiers of localover the putative matching region, and $f(d)=[1-\textrm{tgh}({(d-d_c)/\Delta})]/2$ is a sigmoidal distance weighting factor where $d_c=4$Å and $\Delta=2$Å.

Notice that, as for the RMSIP, the measure (\[eqn:score\]) is independent of the choice of the bases spanning the linear space of the top 10 essential dynamical modes.

The sought dynamics-based alignment is accordingly obtained by maximizing the measure of eq. \[eqn:score\] (after a suitable $n$-dependent regularization, see ref. [@Zen:2008:Protein-Sci:18369194]) over the space of possible amino acid pairings in the two proteins, and finally by assessing its statistical significance by comparing it against a null reference case.

Clearly, the combinatorial space of matching amino acids is very large and, because each attempted alignment involves the re-calculation of the essential dynamical spaces, the computational effort entailed by this comparison is significant and can take several minutes on present-day


at the protein surface [@piana02a; @micheletti04; @piana02b; @McCammonAIDS], poses the question of whether dynamics-based alignments can be used to identify further relationships between proteases that are elusive to the pure structural comparison. The possibility to do so is illustrated in Fig. \[fig:hiv\_bace\_dyn\], which illustrates the dynamics based alignment of HIV-1 PR and endothiapepsin.

![image](Figures/dyn_bace_hiv_composite.png){width="90.00000%"}

Following the spirit of ref. [@enzo_JACS], we have used the Aladyn algorithm to align all pairs of entries in Table \[tab:proteases\]. In addition to the previously mentioned significant structural pairings, the Aladyn algorithm identifies 8 additional significant alignments ($p$-value $<0.02$, corresponding to the incidence of less than one false positive in the set of all pairwise alignments of the entries in Table \[tab:proteases\]). These pairs are shown in Fig. \[fig:composita\_dyn\]. Notice that calpain, adenain, atrolysin E, and HIV-1PR (corresponding respectively to@Munz:2010:BMC-Bioinformatics:20398246; @Biggin2012].

![image](Figures/PDZ_apo_holo.png){width="95.00000%"}

They are typically 80-100 amino acids long and adopt an overall globular fold comprising two $\alpha$ helices and 6 $\beta$ strands, see Fig. \[fig:PDZ\]b. The interaction with a partner protein usually occurs through the accommodation of its C-terminal segment in the $\beta_2$-$\alpha_2$ cleft. In fact, the observed mobility of helix $\alpha_2$ relative to the PDZ-domain core has been argued to be important for ligand binding and recognition [@Munz:2010:BMC-Bioinformatics:20398246; @delos; @Kong:2009:Proteins:18618698]. Although PDZ-domains sustain modest structural changes after ligand binding, see panels a and b in Fig. \[fig:PDZ\], experimental and numerical evidence suggest that there exist allosteric pathways running internally to the molecule that signal the binding event to regions that are opposite on the protein surface respect to the binding cleft [@ranganathan99; @Kong:2009:Proteins:18618698; @delos; @Law:2009:J-Am-Chem-Soc:19374353]. While key aspects of


illustrates the good consistency of the essential dynamical spaces of the aligned regions. Interestingly, the contribution of the various corresponding amino acids to the good RMSIP value, which is equal to 0.74, is rather uneven.

This is illustrated in Fig. \[fig:PDZ\_alignments\]b which portrays the residue-wise contribution to the mean square inner product, $Q_i$ (see eq. \[eqn:local\_msip\]) along with the mean-square residue fluctuations. It is seen that the $Q$ profile is peaked in correspondence of the loops $L_1$, $L_2$, $L_3$ and $L_4$ which are also associated to peaks of the crystallographic B-factor profiles. Although the comparison of computed mean-square fluctuations with B-factors is not perfectly transparent (the latter are affected by crystal packing and disorder [@Frauenfelder:1988:Annu-Rev-Biophys-Biophys-Chem:3293595]), the accord of the two sets of peaks is consistent with the intuition that, given theautomatically compared is not identified by sequence or structural alignments, but by matching the fluctuation (or mode) amplitude profile itself, as first envisaged by Keskin [*et al.*]{}[@keskin]

A key ingredient of this comparative approach is the use of the isotropic Gaussian network model[@bahar97]. Because this phenomenological model does not possess the full rotational-translational invariance of the three-dimensional elastic networks, its essential dynamical spaces have a one-dimensional character. By restricting considerations to the one-dimensional profile of a single mode (or of the mean-square fluctuation) Tobi used a dynamics-based programming strategy to identify corresponding amino acids for various pairs of proteins.

Significant matches were reported for pairs of proteins with different overall structural organization. Consistently with the isotropic character of the elastic network model, the lowest energy mode of these matching proteins


protein conformer, can only limitedly capture and describe large-amplitude motions in such complexes. Furthermore, the very same calculation of essential dynamical spaces from extensive MD simulations can be problematic because they rely on the use of rigid-structural alignments which cannot well superimpose the visited conformers over all their amino acids.

At least for some proteins with mobile subdomains, using internal angular coordinates instead of Cartesian displacements can provide a viable alternative for describing the large-amplitude protein motion[@Mendez:2010:Phys-Rev-Lett:20867208; @Orozco:2011:Adv-Protein-Chem-Struct-Biol:21920324; @LopezBlanco:2011:Bioinformatics:21873636].

The fact that suitably-defined angular coordinates can be used for comparing the dynamics of proteins articulated in several domains was recently illustrated by Morra [*et al.*]{} in ref. [@Morra:2012:PLoS-Comput-Biol:22457611]. This study considered three homodimeric multidomain HSP90 chaperones, namely mammalian Grp94, yeast Hsp90 and [*E.coli*]{} HtpG. The three chaperones, which are represented inis interesting to observe that, as in the study of Zen [*et al.*]{} [@Zen:2008:Protein-Sci:18369194] described in the previous section, the analysis of Hensen [*et al.*]{} [@Hensen:2012:PLoS-One:22606222] has highlighted the possible existence of appreciable dynamical similarities in proteins with limited structural relatedness. The example offered by the authors pertained to the pairing of two hydrolases, serralysin and rhizopuspepsin (PDB codes 1sat and 2apr). Their structural alignment is non-significant according to DALI statistical criteria while in the dynamic metric space considered by the authors they have a strong dynamical proximity. Consistently with this finding the Aladyn alignment of this pair, which involves 79 amino acids) is statistically significant too as the observed RMSIP=0.66 and the associated p-value is 0.025.

Finally, by examining the dynamic fingerprint of functionally-related proteins Hensen [*et al.*]{}[@Hensen:2012:PLoS-One:22606222]


Plugged in Rating

This unique Plugged In Rating for families is based on a movie's content elements
and worldview, not necessarily its artistic qualities. The more the green fills
the plugs, the more family friendly a movie is.

Walk the Line

A young Johnny Cash, growing up in Depression-era Arkansas, loves listening to music on the radio with his older brother, Jack. The boys’ sharecropper dad, whose days consist of hard toil in the cotton fields, doesn’t see the point of music at all, though. And he clearly favors Jack. So when Jack is accidentally killed, the grief-stricken father indirectly blames Johnny.

Johnny grows up and joins the Air Force, where he teaches himself to play guitar and begins to write songs of heartache and despair. He marries a girl he barely knows, Vivian,Parvovirus infection in pregnancy.
A case is presented of serologically proved parvovirus infection leading to intrauterine death of the hydropic fetus at 20 weeks' gestation. Although serologic studies of the population indicate that 30-50% of women are susceptible to parvovirus infection, the prevalence of parvovirus infection in pregnancy and the consequences of such an infection are not well understood. A review of reported cases in the world literature indicates a high risk to the fetus once the virus crosses the placenta.


you’re up there, you can stay as long as you want, so wrap up warm and watch night fall over Manhattan. It’s honestly one of my favourite memories of all time!

5. Times Square

Having watched darkness descend, it was time for some neon. Times Square is only just over half a mile from the Empire State Building, so we walked. We spent a little while in front of one of the big screens trying to get our face on it, which was not normally the sort of thing we would go for – but it was so much fun and I’m glad we did. Here is a picture of our achievement:

It took about 20 minutes to centre ourselves in the heart We are the bespectacled pair right in thethe best!

TIP: If you leave eating until a little later, you won’t have to wait for a table and it will save you valuable time.

Day Two: Full Day in New York City

7. Helicopter Ride

So this is a little unusual for a 2 night stay, granted, but it was a birthday present from me to my husband. It was a misty day, but New York still delivered in the haze, and hovering over the Statue of Liberty was wonderful – a truly unique viewpoint.

TIP: If you only have 2 days, don’t take the helicopter unless it is a specific dream that you’ve always wanted to fulfil. It will take up a whole morning, and is really expensive. If you decide to do it, book ahead online to avoid disappointment


a brilliant red colour and the female with a bright red beak (you can just make her out behind the blind). These birds are native to North America all year around, but in the Spring will you see males and females together foraging for nesting materials, and occasionally ‘kissing’ when the males give the females the materials they have found.

5. You can experience winter and summer all in one day

The Spring in New England offers variable weather. One minute it can be baking hot, the next, cold and windy. Layers are definitely the way forward in this part of the US. We went up the Mount Washington Auto Road in New Hampshire, not quite to the summit as it was still very snowy at the top and theI glanced over at a big lake – and spied a portaloo! Oh, praise be! We pulled over by what turned out to be Lake Overholser (actually a reservoir) and took advantage of the rudimentary facilities…and the peaceful view over the lake.

Relieved (in a slightly different way this time), we set off through the towns of El Reno, Weatherford and Elk City with a brief stop at the Route 66 Museum in desperate need of a coffee. They didn’t sell coffee.

We continued on through Oklahoma passing many a derelict motel and cafe that in days gone by would have warmly greeted us with hot coffee and friendly atmosphere.

Still caffeine and sugar deprived, we arrived in Texola, the last town in Oklahoma before you cross into Texas. Now this


sections were examined microscopically under an optical microscope (Olympus BX50, Japan). The stages of hepatic inflammation/necrosis (I score) and liver fibrosis (F score) were scored by one histopathologist (with 16 years of liver pathology) using the METAVIR classification system \[[@CR32]\] and blinded to the results of the group and MR.

Statistical analysis {#Sec7}
--------------------

Interobserver reproducibility was assessed by measuring intraclass correlation coefficients (ICC) \[[@CR33]\]. Statistical analysis was performed using independent sample *t* test, and Mann-Whitney U test for two group comparisons of the functional MR parameters and biochemical parameters. The Pearson and Spearman correlation coefficient was calculated to assess the correlations between functional MR parameters and biochemical parameters. The data were analyzed using SPSS 17.0 software (SPSS, Chicago, IL, USA), *P* \< 0.05 was considered statistically significant.

Results {#Sec8}
=======

Interobserver agreement of functional MR parametersdecreased blood flow and low oxygenation level can result in increased R2\* value. For the first time, we attempt to evaluate the feasibility of characterization of hepatic WIRI in a rabbit model by using BOLD MRI. To avoid the documented effects of increasing glucose levels on blood flow \[[@CR36]\] and R2\* \[[@CR37]\] in the liver, all rabbits were imaged in fasting conditions in our study. We found that when compared with control group, Dfast decreased 25.01 ± 16.69% in model group, while R2\* increased 36.38 ± 18.31%. Therefore, combined application of BOLD MRI and IVIM technique, which can evaluate tissue perfusion without using of contrast agent, provide a better understanding both tissue oxygenation states and tissue perfusion, and in-depth assessment of hepatic WIRI pathophysiological changes.

Our study has certain limitations. First, we studied


a small number of rabbits. However, this was an exploratory pilot study and herein we reported our preliminary findings. Second, hepatic WIRI is a dynamic process which is intrinsically time-dependent, while the model in this study was characterized only at a specific time frame. A longitudinal follow-up study may reveal the changes of disease evolution, which should be investigated in further studies. Third, we assessed only steady-state normoxia BOLD imaging rather than using extrinsic challenges, such as inhaled carbogen gas or 100% oxygen \[[@CR23], [@CR28], [@CR38]\]. However, the use of extrinsic gas stimulation is technically difficult and time consuming. Our results demonstrated a statistically significant difference, which mean room air was sufficient to draw conclusions.

Conclusions {#Sec14}
===========

IVIM, DTI, and BOLD MR imaging are useful and noninvasive techniques for evaluatingthe rest of the rolling elements. The larger rolling elements and the rest of the rolling elements are arranged in alternating sequence with one another but it is also possible to arrange them in any other order.
The invention will now be described with reference to one example of embodiment illustrated in the appended drawings.


   libopenni2-dev \
      libproj-dev \
      libqhull-dev \
      libqt5opengl5-dev \
      libusb-1.0-0-dev \
      libvtk${VTK_VERSION}-dev \
      libvtk${VTK_VERSION}-qt-dev \
      qtbase5-dev \
      software-properties-common \
 && rm -rf /var/lib/apt/lists/*

# Eigen patch (https://eigen.tuxfamily.org/bz/show_bug.cgi?id=1462) to fix issue metioned
# in https://github.com/PointCloudLibrary/pcl/issues/3729 is available in Eigen 3.3.7
# Not needed from 20.04 since it is the default version from apt
RUN if [ `pkg-config --modversion eigen3 | cut -d. -f3` -lt 7 ]; then \
     wget -qO- https://gitlab.com/libeigen/eigen/-/archive/3.3.7/eigen-3.3.7.tar.gz | tar xz \
     && apt install -y libblas-dev \
  **15** at a good yield. The key intermediate that had been used in the authors\' synthesis of phrymarolin II (**5**), **15**, was subjected directly to an acid-promoted replacement reaction with phenol **22** to afford the coupling product with desired stereochemistry at 15% yield. Then, a simple acylation reaction of the above product completed the asymmetric total synthesis of (+)-phrymarolin I (**4**).

In 1998, Ishibashi and Taniguchi reported their asymmetric synthesis of (+)-haedoxin A (**1**, Ishibashi and Taniguchi, [@B8]). While the core strategy followed the concept of Taniguchi\'s previous synthesis, this new synthesis featured the use of chiral synthons to avoid stereochemical correlation problems. As shown in [Figure 2C](#F2){ref-type="fig"}, chiral compound **33** was first prepared *via* an optical resolution strategy from (±)-**31**. This synthon was coupled with another chiral building


and benzyl trichloroacetimidate **48**, followed by a Swern oxidation, provided the key intermediate **50** at 98% yield. Ketone **50** was then submitted to the photochemical condition developed by Kraus and Chen. In this event, a new C-C bond was formed between the irradiated benzylic position and the furan carbonyl in a diastereoselective manner to give furofuran **51** and **24** at 40% total yield. Finally, **51** was transformed into the desired natural product (+)-phrymarin I through a simple acylation reaction. With this concise synthetic route, the authors also completed the synthesis of (+)-paulownin and (+)-phrymarin II. It is noteworthy that compound **24** could serve as a key intermediate in the synthesis of (+)-phrymalorin I. However, the diastereoselectivity of the key reaction did not favor this intermediate.

Summary and Further Prospects[Analysis of influenza surveillance from 2000 to 2007 in Gansu province].
To study the epidemical characteristics of influenza in Gansu province from 2000 to 2007, and to discuss the changes of the predominant strains of influenza virus. This study provide scientific basis for defending influenza effectively. Influenza surveillance, collecting the pharyngeal swab specimens from influenza patients of national surveillance hospital and unsure influenza epidemic situations, inoculated on MDCK cell culture to obtain the influenza virus strains. 2001.10-2007.12, collecting the 6383 specimens, 943 influenza virus strains were isolated, positive rate was 14.77%, 218 strains were A1 (H1N1 ), 352 strains were A3 (H3N2), 312 strains were B subtype of Victoria and 61 strains were B subtype of Yamagata. There were totally 61 outbreak of influenza-like cases were observed in Gansu


the frigid North Atlantic on one side and fields of snow-covered lava rock on another. To the east looms Snaefellsjokull, a dormant volcano blanketed by a glacier. There’s only one road back to civilization.

This is where the world’s only bank chiefs imprisoned in connection with the 2008 financial crisis are serving their sentences, Bloomberg Markets magazine reports in its forthcoming issue. Kviabryggja is home to Sigurdur Einarsson, Kaupthing Bank’s onetime chairman, and Hreidar Mar Sigurdsson, the bank’s former chief executive officer, who were convicted of market manipulation and fraud shortly before the collapse of what was then Iceland’s No. 1 lender. They spend their days doing laundry, working out in the jailhouse gym, and browsing the Internet. They and two associates incarcerated here—Magnus Gudmundsson, the ex-CEO of Kaupthing’sGrape Pomace as a Promising Antimicrobial Alternative in Feed: A Critical Review.
Antimicrobial resistance is among the most urgent global challenges facing sustainable animal production systems. The use of antibiotics as growth promoters and for infectious disease prevention in intensive animal-farming practices has translated into the selection and spread of antimicrobial resistance genes in an unprecedented fashion. Several multi-resistant bacterial strains have been isolated from food-producing animals, thus constituting an alarming food-safety issue. Many industrial byproducts with potential antimicrobial properties are currently being investigated to identify empirical and affordable solutions/alternatives that can potentially be used in feed for animals. Grape pomace is among such byproducts that gained the attention as a result of its low cost, abundance, and, most importantly, its bioactive and antibacterial properties. This review discusses the


you use for scheduling, whether it is paper or electronic. Be specific and include everything you can think of: work or school hours, meetings, errands, outings, dates, etc. Add everything you know you need or want to do.

2

Determine any free time. You may have an hour between getting off work and meeting your friends for drinks, or you may be able to take an extra long lunch break in the middle of the day. See what will work for you, and remember it does not have to be the same time, or the same amount of time, every day.[1]

3

Be realistic. You know yourself and your preferences better than anyone else. If you hate getting up early, don’t schedule a workout at 5 a.m. or your routine will likelyweek. Also, alternate the types of workouts you do each day to keep your workouts motivating and fun. For example, try a kickboxing class one day and swim laps the next. For tips from our Fitness reviewer on how to build your endurance when you first start working out, read on!


Characterization of the recurrent translocation t(1;1)(p36.3;q21.1-2) in non-Hodgkin lymphoma by multicolor banding and fluorescence in situ hybridization analysis.
Aberrations of chromosomal bands 1p36 and 1q11-q23 are among the most common chromosomal alterations in non-Hodgkin lymphoma (NHL). In this study, 16 cases of NHL showing recurrent unbalanced translocation t(1;1)(p36;q11-23) by G-band analysis were selected for further analysis. To delineate the exact breakpoints, multicolor band analysis for chromosome 1 (M-BAND1), and locus-specific fluorescence in situ hybridization (LS-FISH) using human genome designated BAC clones were performed. In all but one dicentric case, the breakpoint was determined to involve chromosomal bands 1p36.3 and 1q21.1-2. LS-FISH analysis for the TP73, MEL1, SKI, and CASP9 loci at 1p36, and the loci IRTA1, IRTA2, BCL9, AF1Q, JTB, and MUC1 at 1q21, verified the MBAND1 results andfemale privilege?” White men also have a sense of entitlement when they’re saying crazy shit.

I’m not sure the tendency to “forgive”/ignore her views because she’s “hot” is a form of privilege. It seems like the same phenomenon we see when progressive views are “forgiven”/ignored in favor of attention to the speaker’s body.

Yes, reading down the list of bullet points, some of the points filed under “white female privilege” clearly belong under “white privilege”, while things such as “seen as the embodiments of value and purity” seem like a privilege at first and can be in some circumstances, but are actually a disadvantage in the overall scheme of things. But I do agree that white privilege works differently based on gender. Being seen as “the default and the ideal


I can tell you for a *fact* that if I were a woman of color OR a white man, my tears would not have been attended to with “there there now, gotta cater to Jan’s hurt feelings” to the point that the black women whose toes I had just stepped on wound up apologizing to me – when it was I who should have apologized immediately, instead of bursting into tears.

Yes, being a woman (and trans) means I’m oppressed by sexism (and transphobia), but I nevertheless used gendered expectations (that only women cry, and do so because we’re supposedly weak) and cissexist expectations (trans women are hyper-feminine, therefore are hysterical) to totally utterly derail the situation and turn the entire room’s attention away from the black woman thatone white, one black. The white woman is privileged because she is WHTE. Not because she is a “woman” and the black woman is … something other than a woman.

Women as a class are not privileged, period. Under patriarchy, you’re either a man or you’re an afterthought. It’s the whiteness, not the femaleness.

Jadey: I think part of it is that privilege is not about making any individual’s life awesome – it’s about supporting a social hierarchy. Just because some of the ways in which white women are privileged still leave them at a disadvantage compared to white men (assuming they are matched on every other dimension of privilege), that doesn’t negate their advantage over a woman of colour. It’s the power of the kyriarchy to put people in


pack in terms of advantage and comfort. But they still had the jobs, which was more than the black women were getting. The black men as well weren’t exactly CEOs (manual labour, I’m assuming) and probably earned less and had less capacity for promotion and advancement than a white male peer in the same job. But they still had the jobs.Privilege is a system. It’s not a gift or a boon or a reward to an individual which makes their life happy and gay (although if you happen to have privilege on pretty much all of the really socially-important axes, your life probably doesn’t suck all *that* hard). Privilege is often something that is only noticed in its absence, even among those who are possibly acutely aware oftheir lack of privilege in some aspects of their lives. Personally, I really don’t enjoy the way that Whiteness is constructed as an identity – it does not resonate with me at all on an individual level and I don’t derive any fulfillment from it as an identity. But I am still white and still possess white privilege. A man may be incredibly uncomfortable with what constitutes maleness and masculinity in his society and find it unappealing and unsatisfying, but he is still a man with male privilege. I may be alienated by my own white female privilege, but it’s not about me – it’s about the hierarchical structure of social groups that the privileged identity is meant to support.We often discuss intersectionality in terms of the convergence


‘better than’ equals ‘good’, we’re entering into a realm inhabited by moral relativism where social justice concerns aren’t a really thing at all.

LoriA: I’ve been reading everything that’s been thrown out on this topic, and I still don’t understand how female privilege is legitimate in certain contexts. What I’m getting is this: (white) women having men rush to open doors for them is benevolent sexism and therefore not a privilege in the context of patriarchy; white women having men rush to open doors for them is benevolent sexism but also a legitimate privilege in the context of kyriarchy. That is, black women would be better off if they received some of the small “benefits” of benevolent sexism as it’s afforded to white women. I’m sorry, but I justdo not understand how this is truer than just saying “actually, we’d all be better off if benevolent sexism didn’t exist.” If it is, then every example of ‘positive’ discrimination must be a good thing? Black men being treated as hyper-sexual ‘mandingos’? Actually a small privilege when compared to desexualized Asian men. Disabled people being patronized by having the abled help them when they don’t ask for it? Bad, unless we’re comparing it to the way that the disabled homeless are entirely ignored.I’m sorry, but that is simply not logical. Something being “not as bad as” does not make it “good” and therefore not a privilege. If we operate on the assumption that ‘better than’ equals ‘good’, we’re entering into a realm inhabited by moral relativism where social


women are seen as real women. WOC are not seen as real women. White women are seen as real women. WOC are not seen as real women. White women are seen as real women.”
I get that. I do. But that’s white privilege as manifested in females, not female privilege of any sort. That’s not semantics, either; there’s a difference between getting a privilege because you’re white (and happen to be female) and getting a privilege because you’re female.

LoriA, it’s not an either/or, but a both/and. Like I said earlier, think of it as a flow chart. How does a woman get a leg up over other people, especially other women, in a competitive, capitalist, white supremacist patriarchy?

Well, if she’s white, she can get a leg up by engagingcan handle it. Note, this isn’t like racialicious. It’s all the regulars who don’t understand white privilege. On this site, white women’s studies majors are at the top and everyone else’s experiences are disregarded. You people should be embarrassed to call yourselves allies and every single POC on the masthead should be ashamed of themselves. As a POC, I am completely done with young “feminists”.

This is truly unbelievable. If white female privilege did not the exist then what the hell was Sojourner Truth talking about? What the hell do you think happened to Emmit Till? Why the fuck is there a stark difference between media coverage of missing white women compared to the coverage of missing women of color? Every action has a cost benefit value, because an


someone who experiences a lot of emotional difficulties due to disability.

That’s very understandable. But…depression, anxiety, bipolar disorder, PTSD, and various other kinds of mental illness aren’t relegated to just women. Or just white women.

Check out this article from the Crunk Feminist Collective on black women and depression. It mentions Fantasia Barrino’s breakdown (although it doesn’t cover the difference between how Fantasia was treated in comparison to famous white women who’ve had similar breakdowns; you might remember that anyway). I found this paragraph especially salient:

Depression has always been problematic for me because it was something the women in my family and household could not relate to or readily admit. Depression was white women’s shit and my uncontrollable tears and obsession with death was met with confusion and shaken heads.they’re poor, disabled, queer, etc. They have privilege over women of color. Some of you refuse to accept this, and I can only assume that you’ve never actually had a discussion with women of color on this issue.

Please don’t expect people of color to change their language to suit your needs. White Women’s Tears is a term WOC describe the privilege that white women have over them.

I guess you can look at privilege lists two ways. “Every single member of this group has ALL of these privileges” or “These are privileges that this group, as a whole, holds over people outside of this group.” If it’s not about you, it’s not about you.

Yes, I’m *that* Andrea Plaid, the woman who wrote the original post at Racialicious.

First of all,


agree that “white privilege” alone is not sufficient if the discussion we’re trying to have is one about how white privilege itself is gendered, and why, and how that looks and how it functions and how white women are complicit in it.

But qualifying privilege as “female” is inconsistent within the framework we’re all used to using, where privilege is labeled according to the benefits a particular facet of your identity affords you over those who can’t claim that identity. Within this framework, “female privilege” would be assumed to refer to privilege that women hold over men. Prefacing “female” with “white” doesn’t erase that implication. It just ends up sounding, well, additive: “white female privilege” seems to imply that white women hold power over people of color as athis is Australia’s dance macabre. Our politicians are blinded by the money the Chinese pump into our economy. In their eagerness to sell the country, from iron ore to real estate, they appear to forget about those of us at home who may not be able to survive in our own country.

Then there’s the slight issue of China’s obvious expansionist ambitions.

Last but not least, could I write this response in China without disappearing into a government run re-education program?

Wake up Australia. We don’t need the Chinese with their communist version of a new age capitalism inspired by a poorly translated novel by Ayn Rand ruining our economy and government. We can do that ourselves.

— Lon Eisenweger

‘Potential +1 Moments’

If Americans or Australians approach China with a sense of fear


autism and their families a sense of support and community. On Wednesday, 200 children with autism gathered in front of the Castle in the Sand Hotel where they were given the opportunity… Read more »

OCEAN CITY — An uptown Ocean City property with a tragic past will be redeveloped with a mixed-use commercial and residential project after resort planners this week approved the site plan for the long-awaited redevelopment. The Planning Commission on Tuesday approved a site plan for an addition to the UpTown Center along the west side… Read more »12.9. This is significantly better than average.Closest monitor was 7.4 miles away from the city center.

City:

12.9

U.S.:

20.0

Particulate Matter (PM2.5) [µg/m3] level in 2013 was 8.15. This is about average.Closest monitor was 14.3 miles away from the city center.

City:

8.15

U.S.:

9.35

Percentage of residents living in poverty in 2015: 14.0%
(4.2% for White Non-Hispanic residents, 32.6% for Black residents, 23.2% for Hispanic or Latino residents, 31.0% for other race residents, 3.3% for two or more races residents)


 MemoryBufferRef ClonedModuleBufferRef(
      StringRef(ClonedModuleBuffer.data(), ClonedModuleBuffer.size()),
      "cloned module buffer");
  ThreadSafeContext NewTSCtx(llvm::make_unique<LLVMContext>());

  auto ClonedModule =
      cantFail(parseBitcodeFile(ClonedModuleBufferRef, *NewTSCtx.getContext()));
  ClonedModule->setModuleIdentifier(TSM.getModule()->getName());
  return ThreadSafeModule(std::move(ClonedModule), std::move(NewTSCtx));
}

} // end namespace orc
} // end namespace llvm
the population. Sometimes it is difficult if not impossible to have a sample that perfectly represents the population you have in mind. Therefore, you must take into account, not only the usual statistical error due to the size of the sample, but also to the selection bias.

A very famous example of this bias is in the Hite report2. The Hide report had a shocking impact on the American society in the seventies and eighties. No doubt of the qualitative importance of doing such a pioner work about sexual relations. However, the quantitative results were completely distorted. For instance, to state that the 70% of the American women, married for 5 years or more, had sexual relations out of the marriage was simply not reliable. The questionnaire was sent


by post mail to one hundred thousand women. Only 4,5% of them were posted back. Therefore, to extrapolate the statistical results from the sample to the total population of women was a clear mistake.

It is important to remark that a number of 4500 is, in principle, large enough for a sample size. 4500 questionnaires from a well randomized population is enough to do inference. But the sampling to ensure that the statistics from the sample are good approximations of the population statistics is the first aspect to take care of. In the Hite report, these questionnaires were not a randomized sample from the population.

2.- The model: It is common to use some independent variables to estimate the value of others, the dependent variables. To establish the relation betweenthem a model is proposed, which is estimated using the data from the sample. Sometimes the model comes from the theory related with the field we are working with, others the relation is established using an ad-hoc model, which becomes a dangerous framework. The first framework is the most recommended situation. Under this framework, we have a theory (that enables to formulate a reasonable null hypothesis) and the data can be used to test the model. Think that whenever we test a hypothesis, rejection means that we have founded empirical evidence against it. The lack of rejection does not mean that the model is correct. No matter how small the error was when the model was applied to the data from the sample. Further work should be made


Easy DIY Pennants

After stumbling on these adorable mini pennants from Three Potato Four, I was stoked about the possibility to give my plants a boost of personality. But as with all our other DIY projects—wood bead candle holder, Michelle’s reclaimed pallet cow, etc—I knew I could easily make these myself. Follow along for this super simple DIY that anyone—even kids—can do in less than an hour. Call that an accomplished afternoon.

Supplies

Step 1

Trace and cutout the pennant pattern from your stiffened felt. You can download our pattern here.

Step 2

Trace and cut out the contrasting edge from your soft felt.

Step 3

Wrap the contrasting felt evenly around the edge and glue in place. For the back, make sure to glue only along the edge to leave a “pocket” for the skewer.

Stepthe time gaps in the ngpvan.7z dossier, Forensicator had postulated that a much larger copying operation had taken place on July 5. The cf.7z documents with modification dates of July 5 seem to originate from this larger copy operation – but display as exactly one hour earlier, indicating a difference in time zone display rather than a different origin. The earliest time in the ngpvan.7z dossier was 18:39; the documents in the cf.7z/OFA directory (152.6 MB) have modification times between 17:34 and 17:38, immediately preceding allowing for the postulated one hour time zone difference:

The cf.7z/Donor Research and Prospecting contains documents with modification dates ranging from March 2015 to July 5, 2016 (plus one 2011 outlier). Some documents were copied in what Forensicator called the “Windows” style, while others,


and so on. Every single Thing
in this legendary region, be it noun, verb or otherwise, is constructed from
the primal verb "lambda".'

~~~
probably_wrong
Yeah, well, I wasn't precisely laughing when I saw it on my Formal Languages
and Computability course...

Check Church numerals:
[https://en.wikipedia.org/wiki/Church_encoding](https://en.wikipedia.org/wiki/Church_encoding)

~~~
bloke_zero
The reason I was laughing was because I could imagine it happening - know I
know it's true I'm not sure how I feel about it.

~~~
jerf
It's a mathematical formalism for minimizing the number of distinct entities
in minimalistic theories of numbers, and providing definitions of "number"
that are as well-defined as possible, which turns out to be harder than it
appears at first blush. In practice, it isn't generally directly used, except
in places where proper facilities for using integers haven't yet been created
(C++ templates at a certain point, some older Haskell type code before ints
become1. Field of the Invention
The present invention pertains to bow-type centralizers used during operations in oil and/or gas wells. More particularly, the present invention pertains to bow-type centralizers used on casing strings or other tubular goods run into said wells.
2. Brief Description of the Prior Art
Drilling of an oil or gas well is frequently accomplished using a surface drilling rig and tubular drill pipe. When installing drill pipe (or other tubular goods) into a well, such pipe is typically inserted into a wellbore in a number of sections of roughly equal length commonly referred to as “joints”. As a well penetrates deeper into the earth, additional joints of pipe must be added to the ever lengthening “drill string” at the drilling rig in order to increase the depth


nature of a writ of habeas corpus under the All Writs Act, 28
U.S.C. § 1651(a). Petitioner claims that his immediate release from confine-
ment is required in light of the Court of Appeals for the Armed Forces’ (CAAF)
decision in United States v. Mangahas, 77 M.J. 220 (C.A.A.F. 2018), because
he was improperly convicted of charges for which the statute of limitations had
expired. In the alternative, he seeks a rehearing so that he may properly raise
the issue of the expired statute of limitations in light of Mangahas.
    We disagree. We find that while we have jurisdiction to consider this peti-
tion, the CAAF’s ruling in Mangahas is one of procedure and therefore without
retroactive effect to a case which had already completed direct appellate re-
view. As such, petitioner is notDenedo, the Court con-
cluded that military courts have jurisdiction over writs of error coram nobis
even after the proceedings are final pursuant to Article 76, UCMJ, because “an
application for the writ is properly viewed as a belated extension of the original
proceeding during which the error allegedly transpired.” 556 U.S. at 912-13.
But because a habeas corpus petition is not an extension of the direct appeal,
the Air Force court concluded that Denedo’s rationale does not apply and does
not extend habeas jurisdiction beyond the finality of Article 76, UCMJ.
    The Army Court of Criminal Appeals reached a similar holding in Gray. In
Gray, the court rejected Gray’s petition for habeas relief because his court-mar-
tial had completed direct review and there was a final judgment as to the le-
gality of the


is unlikely that any such rules ha[ve]
yet to emerge.” Id. at 417 (alteration in original) (citation and internal quota-
tion marks omitted). Indeed, since Teague, the Supreme Court “ha[s] rejected
every claim that a new rule satisfied the requirements for watershed status.”
Id. at 418. See Hill v. Rivera, 2018 U.S. Dist. LEXIS 200223, at *10 (“The [Man-
gahas] rule here is not a watershed procedural rule that applies retroac-
tively.”). We, likewise, reject the petitioner’s claim that Mangahas announced
a “watershed rule.”
    Because we find that Mangahas does not have retroactive application, we
conclude that the petitioner has failed to establish a “clear and indisputable
right to the requested relief.” Cheney, 542 U.S. at 381.

                 ptr[bx+di],CR      ;put a CR at the end of the data
        inc     di
        mov     byte ptr[bx+di],LF      ;put a LF ...
        inc     di

no_last_line:
        push    di                      ;save the # of chars. in the buffer
        push    bp
        mov


left in the buffer from the next line.
;       - Addrs. of the next line in the buffer.
;
; plus, DX has the adjusted length of the string argument.

try_again:
        inc     ds:line_cntr            ;increment line counter
        pop     bp                      ;addrs. of next line in the buffer
        mov     di,bp              the maximum measured total harmonic distortion has been 2% for frequencies far from resonances.

In order to minimize the effect of harmonics, this method has been implemented, also considering harmonics up to their third order according to [Equation (4)](#FD4){ref-type="disp-formula"}: $$\begin{matrix}
{a(t_{i}) = C + A_{0}\cos(2\pi ft_{i}) - B_{0}\sin(2\pi ft_{i}) + A_{1}\cos(4\pi ft_{i}) - B_{1}(4\pi ft_{i}) +} \\
{A_{2}\cos(6\pi ft_{i}) - B_{2}\sin(6\pi ft_{i}) + A_{3}\cos(8\pi ft_{i}) - B_{3}\sin(8\pi ft_{i})} \\
\end{matrix}$$

Obviously only the fundamental frequency components are considered for sensitivity determination. This least squares approximation is performed by means of the singular value decomposition method.

3.. Influence Factors and Corrections
=====================================

The influences in dynamic calibration of force sensors are different from their static calibration. Effects, such as creep, zero drift and hysteresis, will not appear in dynamic calibration. In this section the influences and corrections


decomposition method. This method has been implemented in such a way that the mean squared error *s*^2^ for the approximation can be obtained directly from the software for each measurement.

Each output signal *a* obtained either from the laser vibrometer or a the force sensor has been fitted to a sinusoidal curve with fundamental frequency *f* that takes into consideration harmonics up to the third order. As the maximum total harmonic distortion is 2% it will be enough considering only the first harmonic for uncertainty determination according to [Equation (14)](#FD14){ref-type="disp-formula"}: $$S(t_{i}) \cong A\cos(2\pi ft_{i}) - B\sin(2\pi ft_{i}) + C$$

Applying the least squares approximating philosophy for *n* samples, the coefficients *A*, *B* and *C* are obtained as described in [Equation (15)](#FD15){ref-type="disp-formula"}: $$\begin{array}{l}
{A = \frac{2}{n}\underset{i}{\text{∑}}{S(t_{i})\cos(2\pi ft_{i})}} \\
{B = - \frac{2}{n}\underset{i}{\text{∑}}{S(t_{i})\sin(2\pi ft_{i})}}and zero as the initial value for α. In practice the value for *m* will not be very different from its weighted mean, as *K* is very small, the expression is evaluated in 2π and σ*~i~* are similar among them.

4.4.. System Reproducibility
----------------------------

For dynamic measurements the sensitivity will be independent from the load. This uncertainty contribution considers the lack of reproducibility for the different loads. This may be caused by the mounting and the shaker effects among other effects. [Figures 12](#f12-sensors-14-18454){ref-type="fig"} and [13](#f13-sensors-14-18454){ref-type="fig"} show an example of this lack of reproducibility for the sensitivity modulus and the sensitivity phase, respectively.

This uncertainty contribution will be determined as the standard deviation of the mean for each excitation frequency among the values obtained for the different loads. This determination will be applied


shaker excitation frequency.](sensors-14-18454f8){#f8-sensors-14-18454}

![The figure represents the ratio between the sensitivity modulus and the sensitivity modulus for 200 Hz *versus* the excitation frequency for the Interface 1610 sensor. One curve is for the case without the coupler and the other curve is the case when the coupler is used.](sensors-14-18454f9){#f9-sensors-14-18454}

![The figure represents the phase *versus* the excitation frequency for the Interface 1610 sensor. One curve is for the case without the coupler and the other case is for the case when the coupler is used.](sensors-14-18454f10){#f10-sensors-14-18454}

![The figure represents the two parts of the pressure coupling element, (**a**) and (**b**), one large cylindrical loading mass, (**c**), and the whole assembly, (**d**).](sensors-14-18454f11){#f11-sensors-14-18454}

![Each curve represents the sensitivity modulus for each load *versus* frequency for the Interface 1610 sensor.](sensors-14-18454f12){#f12-sensors-14-18454}

![Each curve represents the sensitivity phase forin the right of any foreign or domestic corporation by the holder or holders of less than five per centum of the outstanding shares of any class of such corporation's stock or voting trust certificates, unless the shares or voting trust certificates held by such holder or holders have a market value in excess of fifty thousand dollars, the corporation in whose right such action is brought shall be entitled at any stage of the proceedings before final judgment to require the plaintiff or plaintiffs to give security for the reasonable expenses, including attorney's fees, which may be incurred by it in connection with such action and by the other parties defendant in connection therewith for which it may become subject pursuant to section sixty-four of this chapter,


violation found is of Section 8(a) (3) which the Board states resulted in a violation of Section 8(a) (1).5 If there was no offense under 8(a) (3) that is the end of the matter. That statute in its pertinent language reads:

9
"It shall be an unfair labor practice for an employer —

10
* * * * * *

11
"(3) by discrimination in regard to hire or tenure of employment or any term or condition of employment to encourage or discourage membership in any labor organization".

12
The Board argues that "discrimination" is the key word of the statute. It refuses to accept the plain language of 8(a) (3) which makes the particular offense encouragement or discouragement of membership in any labor organization by means of discrimination in regard to hire or tenure ofimportance. While our decision in Quaker State Oil Refining Corp. v. National Labor Relations Board, 3 Cir., 119 F.2d 631, had to do with alleged 8(3) violations which arose out of the actions of employees, the underlying thought of that case is quite material here. We there said, 119 F.2d at page 633: "It is quite clear that all of these conversations took place casually in the course of conversations between the individuals concerned. There is no evidence that they had the slightest effect in actually preventing or discouraging membership in the Union." See also National Labor Relations Board v. Public Service Co-ordinated Transport, 3 Cir., 177 F.2d 119.

16
Generally speaking, the proposition that in order to establish an 8(a) (3) violation there must be evidence that the employer's


The price of Republican hubris Republicans could have gotten a far better deal from President Obama last year, if only they hadn't been so cocky

President Obama has apparently decided what price Republicans should pay for their refusal to work with him the past four years: $800 billion.

That’s the difference between the amount of new revenue Republicans put on the table in the summer of 2011, when they pushed the country to the brink of a debt default, and what Obama is now demanding as discussions over the gradual fiscal slope open.

Advertisement:

Obama will hold his first post-election press conference later today, and his press secretary indicated on Tuesday that he would use it to outline his call for $1.6 trillion in new revenue – to be achieved through anend to the Bush rates on income over $250,000, the Buffett Rule, and tightening loopholes. By contrast, as the Aug. 1, 2011, debt ceiling approached, House Speaker John Boehner had proposed a far more modest $800 billion revenue scheme, one that relied on reforming the tax code and not raising rates on the wealthy.

Whether Obama will get all or most of what he’s now demanding remains to be seen. There’s also the question of entitlements. The deal Boehner and Obama toyed with last year included cuts to Medicare, Medicaid and Social Security, and Republicans are now insisting they be included in any new deal. But Obama’s Democratic base is in much less of a mood to play along now, in the wake of their election victory, and is


informative. With a toe injury keeping the former Bolton, current Galatasary man at less then 100 percent fitness, Ibrahimovic is short of teammates who can finish:

The wide players in Sweden’s 4-2-3-1 (probably Rasmus Elm and Larsson) have a combined six international goals in 77 appearance. The duo was also a non-factor on Monday against Ukraine.

Up top, Elmander’s injury forced him to start on the bench. If can’t go 90 on Friday, either Markus Rosenburg (who started on Monday) or Ola Toivonen (who started at left wing) will get the call. That pair has produced a paltry 12 goals in 57 national team appearances.

The paucity of finishers makes Ibrahimovic’s play-making role risky. After playing as a number nine for nearly a decade under Lars Lagerback, Ibrahimovic came out ofled to dozens of allegations of sexual harassment and, in some cases, rape.

Farrow told NPR's Morning Edition today that Weinstein hired several international corporate intelligence firms. One of them called Black Cube describes itself as a select group of veterans from the Israeli elite intelligence units. Here's how Farrow described that operation.

(SOUNDBITE OF ARCHIVED BROADCAST)

RONAN FARROW: In Black Cube's case, that included human intelligence tactics targeting women, targeting journalists, showing up in their lives using fake identities, using fake companies as a front. This was detailed. This was aggressive. And according to the women I spoke to, this was terrifying.

SIEGEL: We're going to talk now with Ronen Bergman, who is an Israeli journalist. He writes for the Israeli paper Yedioth Ahronoth as well as for The New York


// Copyright (c) 2012 The Chromium Authors. All rights reserved.
// Use of this source code is governed by a BSD-style license that can be
// found in the LICENSE file.

// Custom bindings for the experimental.webRequest API.

var chromeHidden = requireNative('chrome_hidden').GetChromeHidden();

chromeHidden.registerCustomHook('experimental.webRequest', function(api) {
  // Returns the schema definition of type |typeId| defined in |namespace|.
  function getSchema(namespace, typeId) {
    var filterNamespace = function(val) {return val.namespace === namespace;};
    var apiSchema = api.apiDefinitions.filter(filterNamespace)[0];
    var filterTypeId = function (val) {return val.id === typeId;};
    var resultSchema = apiSchema.types.filter(filterTypeId)[0];
    return resultSchema;
  }

  // Helper function for the constructor of concrete datatypes of the
  // declarative webRequest API.
  // Makes sure that |this| contains the union of         "lat":37.083333333333336
                }
            }

Pretty straight forward, except that the API is no longer valid/has limits. In order to finish the project I've instead opted to call this API: http://api.openweathermap.org/data/2.5/weather?lat=37.77&lon=-122.419
Which returns the JSON in this format
{
    "coord": {
        "lon": 139,
        "lat": 35
    },
    "sys": {
        "country": "JP",
        "sunrise": 1369769524,
        "sunset": 1369821049


finite surface and bulk conductivities of the insulator. Projectiles entering the capillary at a later stage are deflected by the Coulomb field of a self-organized charge patch close to the entrance of the capillary. Invoking this scenario we are able to reproduce a range of experimental findings, e.g., ion guiding even for large incidence angles, the temporal decrease of transmission during beam-off times, and, in part, a relatively large angular spread of the transmitted beam. We have shown, that these results can be interpreted on the basis of a linear model including transport of deposited charges along the surface without resorting to freely adjustable parameters. Future investigations should address the dependence on material properties. In particular, measurements for other insulating materials (SiO$_2$) should provide for benchmark data andcritical tests for the theoretical framework presented here.

We are grateful to N. Stolterfoht (Berlin) and F. Aumayr (Vienna) for fruitful discussions and for making their results available to us prior to publication.

The work was supported by the Hungarian Scientific Research Found: OTKA Nos. T038016, T046454, the grant “Bolya” from the Hungarian Academy of Sciences, the TeT Grant No. A-15/04, the Austrian Fonds zur Förderung der wissenschaftlichen Forschung, FWF-SFB016 “ADLIS” and by the EU under contract No. HPRI-CT-2001-50036.

[25]{} S. Ninomya, Y. Yamazaki, F. Koike, H. Masuda, T. Azuma, K. Komaki, K. Kuroki, and M. Sekiguchi, , 4557 (1997).

J. Burgdörfer, P. Lerner, and F. Meyer, , 5674 (1991).

K. Tőkési, L. Wirtz, C. Lemell, and J. Burgdörfer, , 020901 (R) (2000); , 042902 (2001).

M. Mannami, Y. Fujii, and K. Kimura, Surf. Sci. [**204**]{}, 44 (1988).

Y. Morishita, R.


Hutton, H.A. Torii, K. Tomaki, T. Brage, K. Ando, K. Ishii, and Y. Yamazaki, , 012902 (2004).

N. Stolterfoht, J. Bremer, V. Hoffmann, R. Hellhammer, D. Fink, A. Petrov, and B. Sulik, , 133201 (2002).

G. Vikor, R.T. Rajendra-Kumar, X. Badel, J. Linnros, and R. Schuch, Invited and contributed papers of the 22nd Summer School and International Symposium on the Physics of Ionized Gases (SPIG2004), http://www.vin.bg.ac.yu/spig2004/Papers/2P09P.pdf.

Gy. Vikor, R. T. Rajendra Kumar, Z.D. Pesić, N. Stolterfoht, and R. Schuch, Nucl. Instr. and Meth. Phys. Res. B [**233**]{}, 218 (2005).

F. Aumayr, [*private communication*]{} (2005).

Y. Kanai, [*private communication*]{} (2005).

Data sheets of Mylar (http://www.dupontteijinfilms.com).

C. Deiss, N. Rohringer, and J. Burgdörfer, submitted to Phys. Rev. Lett., (2005)

N. Stolterfoht, V. Hoffmann, R. Hellhammer, Z.D. Pesić, D. Fink, A. Petrov, and B. Sulik, Nucl. Instr. and Meth. Phys. Res. B [**203**]{}, 246 (2003).

N. Stolterfoht, R. Hellhammer, Z.D.different color control options and different halftoning techniques to be independently applied to text objects, graphics objects and photo objects. In particular, in the 446 and 021 specification, the user has the option of manually selecting the rendering options or allowing the system to apply default rendering options to each different type of object.
However, the systems and graphical user interfaces disclosed in the 446 and 021 patents set the rendering parameter options independently of a particular document. Similarly, the systems and methods disclosed in the 303 patent render each object using the selected set of rendering parameter options for that object""s type, as modified by any provided rendering tags or hints. All of the 303, 446 and 021 patents render each object independently. For documents having many independent


were analyzed using the SAS statistical package (version 9.3). Kaplan--Meier survival curves were plotted using the R software (version 2.14.1; R Development Core Team, Vienna, Austria).Fig. 1(a) Inclusion and exclusion criteria for the cohort study on allergic conjunctivitis. (b) The association between allergic conjunctivitis (AC) and myopia incidence. The cumulative incidence of myopia is shown for individuals with and without allergic conjunctivitis.Fig. 1

2.2. Experimental Study Design {#s0035}
------------------------------

### 2.2.1. AC Animal Model {#s0040}

Three-week-old male Lewis rats (*n* = 10 animals each) were purchased from the National Laboratory Animal Center (Taipei, Taiwan) and maintained in a specific pathogen-free animal facility at China Medical University (Taichung, Taiwan). All animal care and experimental procedures conformed to institutional guidelines. Rats were injected with 100 μg of chicken ovalbumin (OVA; Sigma-Aldrich, St. Louis, MO) and with 200 μLof aluminum hydroxide (Sigma-Aldrich) (200 mg/mL) in their left hind footpad on days 0 and 7 as immunizations, and then challenged once in the conjunctival sac with 250 μg of OVA diluted in phosphate--buffered saline (PBS) on days 14, 21, and 28. There were two control groups. The rats in the "negative group" were given PBS in the immunization stages and PBS in challenge stages. On the other hand, the rats in the "PBS control group" were given OVA in the immunization stages and PBS in the challenge stages ([Fig. 2](#f0010){ref-type="fig"}a). Twenty-four hours after the final challenge with OVA, the refractive errors and axial lengths of the rats were measured and they were then given an overdose of Zoletil (Virbac, Taipei, Taiwan). After the animals were sacrificed, the eyelids and


resistance (Ω)) × membrane area (0.33 cm^2^). Changes in TEER for each treatment were calculated: change in TEER (%) = TEER (Ω cm^2^)/initial TEER (Ω cm^2^) − 100 (%). Apical and basolateral media were collected to measure the levels of TNF-α, IL-6, and IL-8. One milliliter of basolateral media was used to treat HREpiC seeded in 6-well plates.

### 2.2.7. Immunohistochemistry (IHC) {#s0070}

Eyelids and conjunctivae were fixed overnight in 4% paraformaldehyde in phosphate buffer and embedded in paraffin. Tissue blocks were sectioned at 8-μm thicknesses and collected on clean glass slides. Sections were deparaffinized, immersed in hydrogen peroxide for 30 min, and blocked for 1 h at room temperature in PBS containing 5% normal goat serum, and then incubated overnight at 4 °C with the specific primary antibody against eosinophil major basic protein (MBP; Bio-Rad / AbD Serotec Cat\# MCA5751 RRID:[AB_10671914](nif-antibody:AB_10671914){#ir0010}),from 2.30 (95% confidence interval \[CI\]: 2.24--2.36) for subjects aged 1--6 years to 2.56 (95% CI: 2.43--2.70) for those aged 7--12 years and to 6.08 (95% CI: 3.79--9.76) for those aged 13--18 years in the AC group compared to the non-AC group (p for interaction \< 0.001). Urbanization level-specific analyses showed that the adjusted HR of myopia increased in the AC group, but not in the non-AC group, regardless of living in areas with the least urbanization.Table 2Incidence rates and hazard ratios for myopia in children with allergic conjunctivitis (AC) compared with children without AC stratified by demographic factors in Cox proportional hazard regression.Table 2VariablesNon-AC groupAC groupaHR[a](#tf0020){ref-type="table-fn"} (95% CI)NEvent no.Person-yearsIRNEvent no.Person-yearsIRAll58,74710,474554,90318.958,74721,285469,53745.32.35 (2.29--2.40)[⁎⁎⁎](#tf0025){ref-type="table-fn"}Sex Girl26,1335105238,39821.426,1339694201,61248.12.20 (2.13--2.28)[⁎⁎⁎](#tf0025){ref-type="table-fn"} Boy32,6145369316,50517.032,61411,591267,92543.32.49 (2.41--2.57)[⁎⁎⁎](#tf0025){ref-type="table-fn"}Age, years 1--637,1488420390,33521.637,14816,401324,22650.62.30 (2.24--2.36)[⁎⁎⁎](#tf0025){ref-type="table-fn"} 7--1219,6412034157,73012.919,6414766138,75934.42.56 (2.43--2.70)[⁎⁎⁎](#tf0025){ref-type="table-fn"} 13--1819582068382.921958118655218.06.08 (3.79--9.76)[⁎⁎⁎](#tf0025){ref-type="table-fn"}Urbanization Level 1 (highest)21,4643690203,24518.221,4647820170,84945.82.46 (2.37--2.56)[⁎⁎⁎](#tf0025){ref-type="table-fn"} Level 218,7543411177,18719.318,7546788150,29045.22.30 (2.21--2.40)[⁎⁎⁎](#tf0025){ref-type="table-fn"} Level 310,9352023102,11319.810,935387987,51744.32.19 (2.07--2.31)[⁎⁎⁎](#tf0025){ref-type="table-fn"} Level 4 (lowest)7594135072,35818.77594279860,88246.02.42 (2.27--2.58)[⁎⁎⁎](#tf0025){ref-type="table-fn"}[^6][^7][^8]

[Table 3](#t0015){ref-type="table"} shows the association between


and COL-I in the retinas of PBS and OVA groups were compared to those in the negative group. ANOVA was used to determine significant differences (*p* \< 0.0001) and Sidak\'s multiple comparisons tests were used for paired comparisons between PBS- and OVA-treated eyes. *P* \< 0.05 was considered statistically significant. \*: indicates a significant difference.Fig. 4

### 3.2.3. Up-regulation of Inflammation Promotes the Progression of Myopia {#s0100}

Mast cells play a key role in inducing AC signs and symptoms. Mast cells express FcεRI, a high-affinity IgE receptor. The binding of IgE with specific allergens (pollens, animal dander, or dust mite allergens) activates mast cells and induces degranulation. This causes the release of inflammatory mediators, IL-6, and TNF-α, inflammatory cytokines to initiate ocular inflammation. To determine whether increased inflammation in the ocular surface promotes myopiathe results.

In conclusion, AC-induced retinal inflammation may promote myopia progression. From epidemiological observations, children with AC had a higher risk for myopia. In an animal model, ocular surface inflammation resulting from mast cell degranulation, altered the tight junction of the cornea, initiated the secretion of inflammatory cytokines in the cornea, and subsequently caused retinal inflammation, which in turn promoted myopia progression ([Fig. 8](#f0040){ref-type="fig"}). Inflammation in the etiopathogenesis of myopia remains under-estimated. In this study, through a population-based study and an animal model, we demonstrated a different mechanism of the development of myopia from previous research. Future epidemiologic studies in different geographic regions and experimental studies will be needed to validate the findings of this study and to provide a more comprehensive understanding of the underlying mechanisms of myopia.

Conflicts


Taiwan (CMU106-ASIA-24), Tseng-Lien Lin Foundation, Taichung, Taiwan, Taiwan Brain Disease Foundation, Taipei, Taiwan, Katsuzo and Kiyo Aoshima Memorial Funds, Japan, and the Bureau of Health Promotion, Department of Health, R.O.C. (Taiwan) (DOH99-HP-1205). This work was also supported by CMU under the Aim for Top University Plan of the Ministry of Education, Taiwan. The sponsor or funding organization had no role in the design or conduct of this research.

Supplementary data to this article can be found online at <https://doi.org/10.1016/j.ebiom.2018.01.024>.

[^1]: Chang-Ching Wei and Yung-Jen Kung contributed equally to this work.

[^2]: Abbreviation: OR, odds ratio; CI, confidence interval; SD, standard deviation.

[^3]: Model adjusted for sex, age (continuous), urbanization, parents\' occupational status and allergic disease.

[^4]: Student\'s *t*-test.

[^5]: *p* \< 0.001.

[^6]: Abbreviation: AC, allergic conjunctivitis; IR, incidence rates, per 1000 person-years; aHR, adjusted hazard ratio;Furthermore, the SF2 and D0 values of the 28 CD3+ cases were significantly higher than the SF2 and D0 values of the 14 CD3- cases (SF2: 0.441 +/- 0.048 versus 0.189 +/- 0.045, p = 0.002; D0: 189.6 +/- 26.3 cGy versus 108.7 +/- 18.2 cGy, p = 0.047) and CD3+ cases had smaller alpha values than CD3- cases (0.454 +/- 0.087 versus 0.765 +/- 0.152, p = 0.06). Thus, clonogenic cells from CD3+ T-lineage ALL/NHL patients were more resistant to radiation than clonogenic cells from CD3- T-lineage ALL/NHL patients. In the clinical arm of our study, 33 T-lineage ALL/NHL patients received autologous bone marrow transplants during remission. Pretransplant conditioning consisted of total body irradiation combined with high dose chemotherapy. The expression of CD3 antigen predicted the outcome


(median relapse-free interval = 74 days) for CD3+ patients (n = 19) and 63 +/- 17% for CD3- patients (n = 8) (p = 0.006).(ABSTRACT TRUNCATED AT 400 WORDS)Introduction {#s1}
============

Hydrogen sulfide (H~2~S) is a gasotransmitter that modulates neuronal excitability and synaptic transmission in the peripheral and central nervous systems (Kimura, [@B25]). It is produced by three main enzymes: Cystathionine γ-lyase (CSE) predominates in peripheral tissues, while cystathionine β-synthase (CBS) and 3-mercaptopyruvate sulfurtransferase (3MST) are the major contributors in the CNS (Abe and Kimura, [@B1]; Yang et al., [@B51]; Kimura, [@B25]). Environmental H~2~S is a long-recognized human toxin that increases breathing frequency at moderate concentrations. High concentrations decrease frequency and are also associated with respiratory and cardiac irregularities and coma (Beauchamp et al., [@B6]; Reiffenstein et al., [@B37]). High doses inhibit the activity of inspiratory networks isolated *in vitro* (Greer et al., [@B18]) and cause apnea and death within minutes. However, the lethal actions of exogenous H~2~S


of C4, XII and phrenic nerve activities were analyzed using Clampfit (v9.2, Molecular Devices, Sunnyvale, CA) (for the *in vitro* data) and LabChart (AD Instruments, Sydney, Australia) (for the *in vivo* data). Peak detection was performed to generate burst frequency and amplitude values. For each experiment, values were normalized relative to control (pre-drug or pre-stimulus) levels, and expressed as mean ± standard error of the mean (SEM). Statistical comparison of means was performed using a one-way or two-way repeated measures ANOVA followed by the Tukey post-test (Systat Software, Inc. SigmaPlot 11.0 for Windows). Values of *p* \< 0.05 were assumed significant. Group data are presented as box plots in which: the center line shows the median; box limits indicate the 25th and 75th percentiles; whiskers extend to minimumand maximum values, and; crosses represent sample means.

Results {#s3}
=======

Modulation of *in vitro* inspiratory burst activity by endogenous H~2~S
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Exogenous H~2~S applied via donors is reported to inhibit inspiratory rhythm *in vitro* through actions in the RTN/pFRG and excite inspiratory rhythm in the preBötC. Activation of both sites simultaneously in medullary slab preparations containing both the RTN/pFRG and preBötC results in a biphasic response to H~2~S (Hu et al., [@B22]; Chen et al., [@B8],[@B9]). To test the hypothesis that endogenous H~2~S modulates inspiratory activity *in vitro*, and that the net effect of H~2~S results from an interaction between differential actions in the preBötC and RTN/pFRG, we compared the effects on baseline inspiratory frequency and amplitude in BSSC (which contains the preBötC and the RTN/pFRG) and rhythmic slice (which contains the


preBötC only) preparations of inhibiting H~2~S production via bath-application of incrementing concentrations of the CBS inhibitor, AOAA. AOAA was increased at 30 min intervals from control (0) to 0.1, 0.5, and 1 mM. The response of a single BSSC to bath application of 1 mM AOAA is depicted in Figure [1A](#F1){ref-type="fig"}. Burst amplitude did not change but inspiratory frequency decreased gradually as the AOAA washed in. The single BSSC preparation (Figure [1A](#F1){ref-type="fig"}) and group data (Figures [1B,C](#F1){ref-type="fig"}; *n* = 7) show that bath-applied AOAA had no significant effect on burst amplitude at any concentration, but caused a significant, dose-dependent decrease in inspiratory frequency. The mean relative burst frequencies were 71.7 ± 3.3% (*p* \< 0.001); 42.9 ± 3.9% (*p* \< 0.001) and 32.5 ± 6.2% (*p* \< 0.001)of control at 0.1, 0.5, and 1.0 mM AOAA, respectively (Figure [1B](#F1){ref-type="fig"}).

![Effects of AOAA on rhythmic inspiratory-related activity recorded from the C4 ventral root of BSSC preparations. **(A)** Representative BSSC preparation showing the time course of how instantaneous frequency, calculated from the recording shown (∫C4, top trace), changes following bath application of 1 mM AOAA (indicated by the black arrow). The time scale of the nerve recordings and instantaneous frequency plots are matched. **(B,C)** Box plots of group data showing the dose-dependent effects on C4 inspiratory burst frequency **(B)** and amplitude **(C)** of AOAA bath-applied alone (*n* = 7), and, in a different group of BSSC preparations, in combination with bicuculline (3 μM, *n* = 8). The effect of bicuculline alone is presented in the control group data


are two pathways through which the formation of the intermediate alpha-ketoglutarate (from glutamate) can enter the TCA cycle, a transamination process catalyzed by an AOAA-sensitive aminotransferase and oxidative deamination catalyzed by an AOAA-insensitive dehydrogenase enzyme (Schousboe et al., [@B42]; McKenna, [@B32]). Neurons and astrocytes differentially manage glutamate-glutamine metabolism (McKenna, [@B32]) and some studies suggest significant dependence of astrocytes on AOAA-sensitive processes (Farinelli and Nicklas, [@B15]). However, tracing of CO~2~ formation from ^14^C-labeled glutamate suggests that formation of alpha-ketoglutarate in astrocytes primarily occurs by the AOAA-insensitive oxidative deamination pathway. Specifically, concentrations of AOAA that almost completely stopped transamination had no affect on the production of ^14^CO~2~ (Yu et al., [@B52]). Nevertheless, we cannot exclude that in our studies inhibition of transaminase activity contributed to the effects of AOAA ona(h(o)).
-3*o
Let j(y) = -15*y**2 + 4*y**2 + 12*y**2. Let v(o) = 24*o**2. What is v(j(i))?
24*i**4
Let l(p) = -4*p**2. Let f(t) = -6*t - 5. Calculate l(f(q)).
-144*q**2 - 240*q - 100
Let a(p) = -p**2. Let k(z) be the first derivative of -4*z**2 + 16. What is k(a(u))?
8*u**2
Let b(k) = -4*k. Let t(r) = -4*r + 20. Let p be t(6). Let o(w) = 2*w - 2. Let u(m) = m**2 - m + 1. Let i(v) = p*u(v) - 2*o(v). Give b(i(h)).
16*h**2
Let z(s) be the second derivative of -5*s**4/12 - 15*s. Let f(t) = -3*t**2. Determine z(f(u)).
-45*u**4
Let f(z) = -z. Let g(c) = 4*c**2 + 6*c**2 - 3*c**2. Give f(g(m)).
-7*m**2
Let g(t) be the first derivative of -t**3/3 - 31. Let h(z) = -27*z**2. Determine g(h(y)).
-729*y**4
Let c(j) = 5*j**2. Let