civil war and subsequent 1922 war with their own peasantry.

On the political front, British PM David Lloyd George tired of blockading the Baltic, and legitimized Soviet trade representatives in order to get orders for British factories. The British signed a trade agreement with the Soviets in 1921 and the German Foreign Office, which had done so much to put Lenin in charge of Russia in 1917, signed a loan deal with the Bolsheviks at Rapallo in 1922, just as their gold stock was running out.

By the late 1920s, moral scruples like Seligman's seemed old-fashioned. U.S. Treasury Secretary Andrew Mellon bought dozens of Old Master paintings from the Hermitage in 1930-31, which helped Stalin finance his war on the Ukrainian peasants. Those fenced goods now form the heart ofa Harvard mission to provide advice to the Russian government of Boris Yeltsin, but quietly went into business for himself in violation of his contract, got caught and fired, but retained the sympathy and protection of his mentor, even as Summers served as Treasury Secretary and president of Harvard. A US District Court judge ultimately found Shleifer and Harvard to have committed fraud. The episode has become a standard item in Russia’s anti-American lore. ...

This is by no means to accuse Summers personally of corrupt involvement in the Rape of Russia. The real issue is that this kind of stuff seldom comes up in the press for criticism. I type "lawrence summers russia" into Google and the first page is by Mark Ames and the second one is


...

One obvious question that I've never seen asked is whether America as a whole would be getting a better return on investment if it was pouring these kind of resources into high potential kids instead.

After all, in every other field, we assume that the best teachers will want the best students. For example, I've never heard anybody criticize Barack Obama for teaching very smart, mostly highly affluent young people at the University of Chicago Law School, instead of choosing to teach struggling law students at a fourth tier law school, of which Chicago has several. Everybody just assumes that that of course it's best for all concerned that a radiantly beneficent being like Obama should exude his ineffable influence all over tomorrow's leaders at the U of CRoller et al and 3,919,719 Wright et al describe the use of a liquid lubricant perfluoroalkylpolyether, which is sold under the trade name KRYTOX.RTM.. Such a liquid lubricant has the advantage that it will creep across the surface, thereby replenishing a portion of the layer which has been removed by abrasion or wear. At room temperatures, this liquid lubricant will protect the surface of the magnetic record for the desired lifetime. However, under higher operating temperatures, or long life applications the lubricant evaporates, thereby reducing the protection. The use of higher viscosity, longer chain molecules of perfluoroalkylpolyether prolongs the life of the lubricant because the evaporation rate is less. However, these higher viscosity lubricants sometimes cause the magnetic head to stick to the magnetic surface when stationary. Upon


and 4 were more effective than PLG in enhancing the binding of ADTN to dopamine receptors. The 5,5-bicyclic thiazolidine lactam peptidomimetic 3 enhanced the binding of ADTN by almost 200%, while the 6,5-bicyclic thiazolidine lactam peptidomimetic 4 enhanced the binding of ADTN by about 75%. These results provide further evidence in support of the hypothesis that the bioactive conformation of PLG is a type-II beta-turn.still rest on my shoulders. Those hands have done this over 20,000 times and they seem reassuringly still attached to a very tall very relaxed Serbian jump master’s body. The same body I’m about to be strapped to and hurled out of a plane with at 200 clicks.

As the plane plateaus I’m a little quieter. It's the 10,000ft drop off point. A few people disappear out the now open door – seemingly to be seen again on the ground below. The engine kicks in again and the plane reassumes its climbing position – 5,000 ft to go. I’m very quiet. Safe hands. They are still there, thank god.

My ears start to pop and we seem to be chugging into the cloudy heavens – the tops of the Remarks


another photon in the $2i$th Fock state. If two DFSEs succeed, Bob obtains the undecohered rotated qubit, for example in the case of (i), as follows; $$\begin{aligned}
&& a^2|H\rangle_s^{(2i-1)}+e^{i\theta_i}d^2|V\rangle_s^{(2i-1)}
\rightarrow
a|H\rangle_s^{(2i-1)}+e^{i\theta_i}d|V\rangle_s^{(2i-1)}
\\
&&\rightarrow
\frac{|H\rangle_s^{(2i-1)}+e^{i\theta_i}|V\rangle_s^{(2i-1)}}{\sqrt{2}}=|+_{\theta_i}\rangle_s^{(2i-1)}.\end{aligned}$$ The more general case is explained in appendix $3$. $\Box$

By using a random walk with an absorbing boundary, we calculate the success probability of the protocol. In the limit of large $\mu$, the success probability of the coherent-light-assisted protocol scales like $O(T)$ asymptotically (see appendix 3).

Next, we show blindness of the coherent-light-based protocol.\
[*Proof:*]{} The information, which Alice sends to Bob, is $\hat{U}_{\rm{PBS}}|+_{\theta_i}\rangle^{(i)}$, $\hat{U}_{\rm{PBS}}|\mu\rangle_+^{(2i)}$, $n$, $\bf{M}$, and $\xi_i$. Similarly to the previous cases, the state, which Alice sends to Bob, is written as $$\begin{aligned}
&&\bigotimes_{i=1}^{N'(n, \bf{m})}\sum_{r_i=0}^{1}\frac{1}{2}\hat{U}_{\rm{PBS}}^{\otimes 2}(|+_{\theta_i}\rangle^{(i)}\langle+_{\theta_i}|^{(i)}\otimes|\mu\rangle_+^{(2i)}\langle\mu|_+^{(2i)}){\hat{U}_{\rm{PBS}}^{\dag\ \otimes 2}}\\
&&=\bigotimes_{i=1}^{N'(n, \bf{m})}\frac{\hat{I}}{2}\otimes\hat{U}_{\rm{PBS}}|\mu\rangle_+^{(2i)}\langle\mu|_+^{(2i)}\hat{U}_{\rm{PBS}}^\dag\end{aligned}$$ in Bob’s point of view. The above state does notA study of 589 consecutive implants supporting complete fixed prostheses. Part I: Periodontal aspects.
Ninety-one consecutive edentulous patients who had been treated by means of fixed prostheses (n = 103) supported by Brånemark implants (n = 589) were examined. Eighteen fixtures (3%) failed to integrate. After a mean loading time of 32 (range 6 to 80) and 38 months (range 5 to 83) for fixtures in the maxilla and mandible, respectively, an additional 12 fixtures lost integration. The cumulative failure rate for individual fixtures after 6 years was 8.4% and 5% for maxillae and mandibles, respectively. Only one patient had to revert to complete dentures. For fixtures in the maxillae, the mean marginal bone loss was 0.7, 0.1, and 0.2 mm for each of the first 3 years of


loading, respectively; for fixtures in mandibles, the corresponding values were 0.7, 0.2, and 0.1 mm. The loss in marginal bone height was equal in both jaws and was not related to bleeding on probing or to the preoperative resorption anatomy.import { html, PolymerElement } from "@polymer/polymer/polymer-element.js";
import "./lib/gitgraphjs/src/gitgraph.js";
/**
 * `lrn-gitgraph`
 * @element lrn-gitgraph
 * For each branch in your repo use the following git command:
 *   ```
 *   git log [branch_name] --format='{"refs": "%d", "commit": "%h", "tree": "%t", "parent": "%p", "subject": "%s", "date": "%cd", "author": "%an %ae"},' --reverse
 *   ```
 * @demo demo/index.html
 */
class LrnGitgraph extends PolymerElement {
  static get template() {
    return html`
      <style>
        :host {
          display: block;
          overflow-x: scroll;
        }
      </style>


branch in this tree is a path from a leaf block to the genesis block; each such branch represents one version of the history of Bitcoin transactions. Each branch must be internally consistent and can never include two conflicting transactions; however, the branches need not be consistent with one another, and one branch can include a transaction which contradicts a transaction in another branch.

Since a single coherent history of transactions is desired, at every point one branch in the tree is considered the valid block chain. It is agreed that every node will consider the longest branch it is aware of as the valid chain (more precisely, the branch representing the most proof of work). If multiple branches are tied, the one of which the node learnt firstconfirmations are needed to have it less than 1%, and 6 confirmations are necessary to decrease the probability of success below 0.1%.

We can see that, for any given probability, as the attacker’s hashrate approaches half of the total network hashrate, the number of confirmations required approaches infinity; for $q\ge0.5$, no amount of confirmations will suffice to decrease the probability, as it is always 100%.

$$\begin{array}{cc}
\includegraphics[width=80mm]{Doublespend4.pdf}&
\includegraphics[width=80mm]{Doublespend5.pdf}\\
(a)&(b)
\end{array}$$

In , we see a detailed tabular reference for the probability of success, for different values of $q$ and $n$.

      q      1         2         3         4


no longer receive any rewards, would quit due to lack of incentive; this will make it even easier for the attacker to maintain his dominance. This will cause either the collapse of Bitcoin or a move to a modified protocol. As such, this attack is best seen as an attempt to destroy Bitcoin, motivated not by the desire to obtain Bitcoin value, but rather wishing to maintain entrenched economical systems or obtain speculative profits from holding a short position.

The exact economics of double-spending is of course highly complex; we will analyze a simple model that aims to highlight the key moving parts.

1.  [A double-spending attack can be carried against more than one merchant. Payments can be simultaneously sent to $k$ different merchants, with the same branch invalidatingbecause it would not be economical to carry out an attack.

We will assume for simplicity that $o=20$, meaning the attacker gives up after finding 20 blocks without successfully catching up, and that this does not impact his probability of success. This is a significant simplification; a more accurate model would determine an optimal stopping point by considering the reward of possibly completing the attack versus the risk of compounding the losses, and use the average blocks found until reaching that point. However, this assumption still preserves the spirit of the results.

This means that discouraging an attack requires that $$v\le\frac{20(1-r)B}{k(\alpha+r-1)}$$

Unlike the adversarial setting, here increasing the time constant $T_0$ slightly improves security with a given number of confirmations, as it increases the effective value of $B$, and hence the


These instruments are conventionally used for the measurement of infrasonic waves also called infrasound, that is to say with very small pressure variations (typically comprised in the range from a millipascal to around a hundred pascals) of which the frequency is less than 20 Hz; these instruments measure the shape of the waves more than the intensity of the corresponding signal. These instruments are of a very different type to that of barometers, which measure the atmospheric pressure, that is to say values of the order of 105 Pa, with an accuracy of the order of about a hundred, or a thousand, pascals.
Infrasonic waves are in particular produced by events, whether or not natural, of which the following (non-exhaustive) list gives an idea of the extent of theArchive for October 2010

Friday, October 29, 2010

I have little boys, but I also babysit a couple of little girls from time to time. So when Gummylump asked us to review some of their award-winning creative play toys, I chose the Bake & Decorate Cupcake Set. You know, so the little girls who come over will have something to play with. Okay, maybe they were really for me. But the good news is that my 5-year-old son adores this set, too!We had about 12 birthday parties in a row one afternoon, after which my son proclaimed that I was now 12 years old. Whew! I thought he was going to do the math and make me much older. I guess it's good he doesn't do much math yet. Anyway,


also leave the ring on it and press the ring down into the cupcake so the recipient would have a little Halloween favor. To make your spider sparkly like it's web, paint a little orange or black Stickles glitter glue on top and let it dry completely.

5. I cut Halloween cupcake wrappers with my Silhouette machine and slipped each cupcake into one. Love this scull design!

These must be the creepiest cupcakes in town!

share this on »

Friday, October 22, 2010

My new nephew arrived yesterday and I am just dying to see, smell, and kiss him. I shipped off his quilt and other baby goodies (like this hat) today, but since his Mama is still recovering in the hospital, I think it's fairly safe to post pictures here without ruiningmy Silhouette machine (using a carrier sheet) which eliminated the drawing and hand cutting steps.

2. Iron on some of your Heat 'n Bond iron-on adhesive to the back (wrong side) of your fabric, following the directions on the packaging.

3. Iron your freezer paper shape shiny-side-down on the front (right side) of the fabric. It doesn't take long to get it to adhere--just a quick pass over the top with the iron.

4. Cut around your template with scissors, then peel off the freezer paper. Save it for the next kid if you want--you can reuse your templates!

That's it! Now celebrate in style! Bonus on this "5" shirt--the light blue thread I picked happened to be glow-in-the-dark thread! I didn't even know they made it, let alone that I had


How to use the echo effect to move between tracks

Top 5 DJ Tips from Point Blank: No.5

Shares

This is a great technique to add to your arsenal and is perfect for moving between songs that might contain unusual outros or intros, perhaps due to spoken word sequences, or pre-recorded crowd noise etc.

Ben Bristow, head DJ tutor at Point Blank - the world's best music production and DJ school - brings you five essential DJ tips, tricks and cheats that could make the difference between a killer set and death on the decks.is most convenient for the structure under consideration. Note that modal damping or resonator damping can easily be added at a later stage, but the bandgap phenomenon is most simply derived in the absence of any damping, without loss of generality. The boundary conditions can be written as $${\mathop{}\!\text{B}}_iw({\boldsymbol{\mathbf{P}}},t) = 0, \quad i = 1, 2, \ldots, p, \quad {\boldsymbol{\mathbf{P}}} \in \partial D \label{eq:bc}$$ where ${\mathop{}\!\text{B}}_i$ are linear homogeneous boundary differential operators of order $[0, 2p-1]$ and $\partial D$ is the boundary of $D$. Equations  -  represent the general boundary value problem of a locally resonant metastructure. Note that, because the force exerted by the resonators depends on the displacement of the structure, the mode shapes of the original structure without resonators (referred to as the “plain structure"


can be used for the material properties of the structure. By substituting this expansion into the governing equation for the structure, applying the periodicity of the resonator locations, multiplying by an exponential, and applying orthogonality, an eigenvalue problem is obtained. This eigenvalue problem must be solved for the eigenfrequencies $\omega$ at every Bloch wavevector in the irreducible region of the first Brillouin zone. It is possible to apply this technique to a general vibrating system whose governing equation is given in operator notation, which is described in more detail in \[sec:pwem\_app\]. For sufficiently small lattice constants, the bandgap from the PWEM dispersion analysis agree well with the edge frequencies in Eq. , as shown in Fig. \[pwem\_comp\] for various types of structures (Fig. \[fig:sys\_schem\]) in which the plain structure (i.e. structurebeam via two screws and nuts, such that there is of spring steel extending past the main beam on both sides, forming two cantilevers. Two cube neodymium permanent magnets are placed at the tip of each cantilever, giving a total tip mass of . The main beam was clamped vertically to an APS long stroke shaker, which excited the beam by base motion. The base acceleration was measured using an accelerometer, and the tip velocity was measured using a Polytec laser Doppler vibrometer (LDV), directed at the tip (free end of the beam) using a small mirror aligned at $45^\circ$ degrees relative to the floor.

The experiment was designed to allow flexibility in the placement of the resonators. Since it is expected that periodicity is not required under the


the point mass (the mass of the clamping hardware) at $x = x_j$. For a finite number of attachments, point masses contribute to the mass matrix of the discretized system in a similar fashion to the resonators, without the frequency dependence.

Uniform Distribution of Resonators and Effect of Mass Ratio {#subsec:uniform_exp}
-----------------------------------------------------------

First we discuss the effect of mass ratio for the case of uniformly distributed resonators. The experimental setup was designed to permit multiple uniform arrangements of resonators, with 36 evenly spaced sets of holes where resonators could be attached. Tests were conducted using $S = 9$, $S = 12$, and $S = 18$ evenly spaced resonators. Although the tuning of the resonators was kept the same for each test, the mass ratio $\mu$ increased as the number of resonatorsIntroduction
============

After our recently published work ([@B2]) on Chinese species of the *Stenus indubius* group, we received new material containing two new species of the group collected from Guangxi and Zhejiang provinces, respectively. These new species are described in the present paper. One of them is closely related to *Stenus paradecens* Tang & Li, 2005 and *Stenus guniujiangense* Tang & Li, 2005. However, the figures in the original descriptions of the latter two species have minor flaws and are poorly printed. Therefore, new and improved illustrations are provided.

Material and methods
====================

The specimens examined in this paper were collected by sifting leaf litters in forests. For examination of the male genitalia, the last three abdominal segments were detached from the body after softening in hot water. The aedeagi, together with other


can store data for a long time, is often used for recording a control program of an electronic apparatus. The flash memory requires a higher voltage than a normal power supply voltage for rewriting a data. As a consequence, the flash memory internally handles a plurality of kinds of voltages of relatively high and relative low voltages. Therefore, it is necessary to balance a circuit handling the relatively high voltage and a circuit handling the relatively low voltage in the operation speed and the voltage.
In U.S. Pat. No. 6,433,583, a CMOS switch circuit of a high voltage transition type, more specifically, a decoder circuit for a non-volatile memory is disclosed. In the CMOS switch circuit, a signal on the output side is fed back to the input side,Lawyer who Met Zygier: He wasn't Depressed

A lawyer who met "Prisoner X" a few days before he died saw no sign of suicidal tendencies.

Contact Editor

Gil Ronen, 14/02/13 22:09

Ayalon jail, where Zygier was held.

Reuters

A lawyer for Israeli-Australian Ben Zygier, who was jailed on security grounds in 2010, told AFP on Thursday he was "shocked" upon learning of the inmate's death, days after they met.

"During the entire conversation, I did not get the feeling this was a person about to take his life," lawyer Avigdor Feldman told AFP regarding the man who came to be called Prisoner X.

"I must say I was shocked when I heard about it (his death)."

Speaking from near the southern resort city Eilat, Feldman described the person he met as focused and "emotionally stable," noting the


film.)

It's a "gorgeous" improvement: Deathly Hallows is "monumental cinema," says Philip Womack at Britain's Telegraph, and its "greatest triumph" is that it overcomes the "deficiencies" of Rowling's novel. In her pages, the climactic final battle at Hogwarts was "a damp squib," failing to "muster the epic feel needed." But the film manages to turn Rowling's fight scene into a "genuinely terrifying spectacle." The film, in turn, becomes a "triumph."

"Harry Potter and the Deathly Hallows, Part 2, review"

And justifies the decision to split the last book into two films: The "squabbling" that plagued Deathly Hallows Part I is a thing of the past, says Todd McCarthy at The Hollywood Reporter. The "narrative balance and refinement," and perfectly orchestrated "massive chessboard of events" in Part II make this film an1930, the Board of Estimate appropriated the complete property through a condemnation proceeding. On July 1, 1930, Parks gained jurisdiction over the park. Originally named Marine Park, Commissioner Stern gave the property its present name in 1985.

Beach Channel Park adjoins Jamaica Bay, an 18,000-acre wetland estuary bordered by the Rockaway Peninsula to the South, Brooklyn to the West, and Queens to the East. The bay has a total area nearly equal to that of Manhattan, and contains numerous islands separated by a labyrinth of waterways. The region also supports meadowlands and two freshwater ponds. The wetlands of Jamaica Bay are protected from the harsh environment of the Atlantic Ocean by the Rockaway Peninsula, and provide a unique environment for both wildlife and urban recreation. The region is host


one targeting *Dpp* (asterisk), transmit mutant alleles to the majority of offspring. Flies expressing sgRNAs targeting *Dpp* in the germline produce few viable offspring and transmitted only a single, in-frame, mutation out of 11 analysed offspring. The same sgRNA construct results in highly efficient mutagenesis in somatic tissues (see [Figure 4](#fig4){ref-type="fig"}), consistent with haploinsufficiency of *Dpp* in the *Drosophila* embryo.](elife-53865-fig1){#fig1}

Next, we tested whether pCFD6-sgRNA^2x^ also mediates efficient mutagenesis in the germline, where some UAS vectors are silenced ([@bib9]; [@bib22]). This is a particularly important application, as it allows to create stable and sequence-verified mutant fly lines, which can be backcrossed to remove potential off-target mutations. We crossed previously described *nos-Gal4VP16 UAS-Cas9.P1* flies ([@bib40]) to sgRNA strains targeting either *neur*, *N*, *cut* (*ct*), *decapentaplegic* (*dpp*) or *Ras85D*. Despite theCas9 expression to optimally balance activity and toxicity. Such a system would ideally allow to modulate Cas9 levels independent of enhancer strength, in order to be compatible with the wide range of available Gal4 lines. We employed a method that uses upstream open reading frames (uORF) of different length to predictably reduce translation of the main, downstream ORF ([@bib16]; [@bib29]; [@bib50]). We created a series of six *UAS-cas9* plasmids containing uORFs of different length, ranging from 33 bp (referred to as *UAS-u^XS^Cas9*) to 714 bp (*UAS-u^XXL^Cas9*, [Figure 2A](#fig2){ref-type="fig"}). When combined with *nos-cas9* these plasmids resulted in Cas9 protein levels inversely correlated with the length of the uORF ([Figure 2B](#fig2){ref-type="fig"}, [Figure 2---figure supplement 1C](#fig2s1){ref-type="fig"}). Reducing the amount of Cas9 protein resulted in a strong decrease in the number of


(XS-L) leading to comparable gene editing that removes Evi from nearly all cells in the Gal4 expression domain. Quantification of staining intensity for Evi protein in the wing pouch (Gal4 on), relative to Evi staining in the hinge region (Gal4 off). N ≥ 6 wing discs. (**E, E'**) CRISPR mutagenesis patterns reflect Gal4 expression history. (**E**) Fluorescence of GFP, which turns over, reflects most recent Gal4 expression pattern. (**E'**) CRISPR mutagenesis, visualized by activation of the CIGAR reporter, is permanent and reveals the Gal4 expression history. Images of a representative wing disc are shown to the left of each panel and average intensity projection of several discs registered to a common template are shown on the right (see Materials and methods). Areas that are CIGAR positive in many discs appear bright, whileheat inactivation for 3 min at 95°C. 3 µl of supernatant were used in 30 µl PCR reactions with primers pCFD6seqfwd2 and pCFD6seqrev2. PCR amplicons were analysed by Sanger sequencing with primer pCFD6seqrev2.

Selection of lethal and viable target genes {#s4-10}
-------------------------------------------

Genes considered 'known lethal' or 'known viable' were chosen based on information available in FlyBase (release FB2018_1). For each gene report we manually reviewed the lethality information available in the phenotype category. We did not consider information based on RNAi experiments, as these typically were performed with tissue-restricted Gal4 drivers and residual expression might mask gene essentiality. Annotations of viability in FlyBase are heavily skewed towards lethal genes, likely reflecting the uncertainty in many cases whether a viable phenotype reflects residual gene activity of a particular allele.

Immunohistochemistry {#s4-11}
--------------------

Immunohistochemistry of


Core Facility, the Zeiss Application Center at the DKFZ and the Nikon Imaging Center at Heidelberg University. Work in the lab of MB is in part supported by grants from the European Research Council (ERC) and the DFG (SFB/TRR186, SFB1324).

Additional information {#s5}
======================

No competing interests declared.

Conceptualization, Data curation, Formal analysis, Supervision, Investigation, Visualization, Writing - original draft, Project administration, Writing - review and editing.

Investigation, generated the sgRNA library.

Investigation, generated the sgRNA library.

Software, Investigation, Methodology.

Software, Investigation, Methodology.

Investigation, Methodology.

Investigation, generated the sgRNA library.

Investigation.

Investigation, generated the sgRNA library.

Investigation, generated the sgRNA library.

Investigation, generated the sgRNA library.

Investigation, generated the sgRNA library.

Investigation, generated the sgRNA library.

Investigation.

Investigation.

Investigation, generated the sgRNA library.

Investigation, generated the sgRNA library.

Software, Investigation, generated essential IT infrastructure.

Conceptualization, Formal analysis, Supervision, Funding acquisition, Investigation, Writing - original draft, Project administration, Writing - reviewand editing.

Additional files {#s6}
================

###### HD_CFD Drosophila lines 2019.

###### Results of the lethality screen.

###### Uncharacterized essential genes.

###### Oligonucleotide sequences.

###### Drosophila strains used.

Data availability {#s7}
=================

All materials are available upon request. All data are contained within the manuscript and associated supplementary material. Transgenic fly lines are available through the Vienna *Drosophila* Resource Center (<https://stockcenter.vdrc.at>). Plasmids will be made available through Addgene (Addgene plasmids 127382--127388).

10.7554/eLife.53865.sa1

Decision letter

Eisen

Michael B

Reviewing Editor

HHMI, University of California, Berkeley

United States

King-Jones

Kirst

Reviewer

Canada

In the interests of transparency, eLife publishes the most substantive revision requests and the accompanying author responses.

**Acceptance summary:**

A persistent challenge in unraveling endogenous gene function is having the tools to determine when and where genes, particularly essential genes, are functioning in vivo. In this manuscript, the authors leverage CRISPR-Cas9 gene editing reagents to create an impressive toolbox to interrogate gene


may not be completely solubilized prior to 2-DE. Efficient solubilization of protein samples is critical for high performance 2-D electrophoresis.

The most critical step in any proteomics study is protein extraction and sample preparation ([@b25-gmb-35-1-sup-353]). Protein extraction methods can vary widely in reproducibility and representation of the total proteome. Better solubilization increases the separation and resolution of gels allowing identification of a higher number of proteins and more accurate quantification of differences in gene expression. Plant tissues, when compared to animal tissues, often contain lower protein concentrations and extraction is often rendered difficult by the presence of interfering compounds, such as secondary metabolites, phenolic compounds, lipids, nucleic acids, the cell wall and storage polysaccharides. Some of these compounds may interact with proteins and give poor resolution and high backgroundthen re-swollen with 20 μL of 10 μg/mL trypsin (modified porcine trypsin, sequencing grade, Promega, Madison, WI, USA) in 25 mM ammonium bicarbonate. Digestion was performed overnight at 37 °C. The resulting tryptic fragments were extracted by sonication in 50% acetonitrile and 5% trifluoroacetic acid. The extracts were dried and then dissolved in 50% acetonitrile and 0.1% trifluoroacetic acid.

MALDI-TOF-MS analysis of tryptic peptides was performed using a Bruker UltraFlexIII spectrometer. Samples were co-crystallized with CHCA matrix and spectra acquired with 40 shots of a 337 nm nitrogen laser operating at 20 Hz. Protein identification was done by searching the National Center for Biotechnology Information (NCBI) non-redundant database using the Mascot search engine and a probability-based scoring system ([@b24-gmb-35-1-sup-353]). The following parameters were used for database searches with MALDI-TOF


                                               35060/6.54    77    14   36       gi\|4930123
  287       Ferredoxin---NADP reductase, chloroplastic; Short = FNR; Flags: Precursor (*Vicia faba*)       40838/8.70    99    18   37       gi\|729479
  335       Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (*Hypserpa nitida*)     Washington is racing to keep up with the rapid development of self-driving cars.

Lawmakers in the House and Senate are crafting a package of autonomous vehicle bills due out this summer, the Trump administration is rewriting federal guidelines for driverless cars and K Street has increasingly ramped up lobbying on the issue.

The effort reflects just how close many believe the emerging technology is to becoming a practical reality for the masses — and policymakers want to make sure they are firmly in the driver’s seat.

ADVERTISEMENT

“This is happening very fast,” Sen. Gary Peters (D-Mich.), one of the members behind the Senate’s legislative effort, told The Hill. “That’s the challenge, is you have a technology that is moving at an exponential rate, and you have public policy and regulations that move


regulators from requiring pre-market approval for autonomous vehicles, allowing up to 100,000 cars per year to be exempt from traditional automobile requirements and establishing a framework for how developers should share test and crash data.

Latta, who chairs the subcommittee, said the goal is to put together a package after gathering feedback from members and get the bill through committee by August.

“We want this to be regular order,” Latta said. “We’ve got to get this right.”

The White House has also taken an interest in the issue.

Transportation Secretary Elaine Chao said the administration is revising the Obama-era guidelines for driverless vehicles, with updated guidance expected in the next few months.

“The pressure is mounting for the federal government to do something,” Chao said during a conference in Detroit earlier this month,There was a statistically significant correlation between EGFR expression and tumor differentiation, and a borderline correlation between Cerb-B2 expression and T stage. No association was found between growth factor expression and tumor depth, lymph node status, extracapsular invasion, recurrence, or survival. Overexpression of EGFR and Cerb-B2 cannot serve as a prognostic factor or predictor of survival and treatment success in SCC of the tongue.


   System.out.println(array[i].getText());
            });

        }

        Scene scene = new Scene(p1, 400, 250);
        primaryStage.setScene(scene);
        primaryStage.show();
    }

    public static void main(String[] args) {
        Application.launch(args);
    }

}

Here i is a property of the Demo instance (i.e. the application instance on which start() is invoked). In the start() method, i is initialized to 0 and then incremented each time you iterate the for loop. The for loop exits whenthere is literally billions of dollars' worth of rare earth elements contained in our nation's coal ash," said Heileen Hsu-Kim, the Mary Milus Yoh and Harold L. Yoh, Jr. Associate Professor of Civil and Environmental Engineering at Duke.

"If a program were to move forward, they'd clearly want to pick the coal ash with the highest amount of extractable rare earth elements, and our work is the first comprehensive study to begin surveying the options," Hsu-Kim said.

The researchers took coal ash samples from power plants located mostly in the American Midwest that burn coal sourced from all over the country, including the three largest sources: the Appalachian Mountains, southern and western Illinois, and the Powder River Basin in Wyoming and Montana. The content of rare earth elements was then


* @
    */
    public static PgpPublicKey ReadPublicKey(Stream inputStream)
    {
        inputStream = PgpUtilities.GetDecoderStream(inputStream);
        PgpPublicKeyRingBundle pgpPub = new PgpPublicKeyRingBundle(inputStream);
        //
        // we just loop through the collection till we find a key suitable for encryption, in the real
        // world you would probably want to be a bit smarter about this.
        //
        //
        // iterate through the key rings.
             PgpEncryptedDataGenerator encGen = new PgpEncryptedDataGenerator(SymmetricKeyAlgorithmTag.Cast5, withIntegrityCheck, new SecureRandom());
            encGen.AddMethod(passPhrase);

            Stream encOut = encGen.Open(output, processedData.Length);

            encOut.Write(processedData, 0, processedData.Length);
            encOut.Close();

            if (armor)
                output.Close();

            return bOut.ToArray();
        }

        private static


people actually want to learn how to protect themselves may be too large a leap. For over two decades, the media has published stories about the dangers of computing -- often with gaping inaccuracies. These stories are rarely calls to action -- instead, they seem designed to reinforce the impression of computers and the Internet as scary technologies. If anything, these stories discourage action, because they make casual users believe that computers and the Internet are far too dangerous and complicated for them to tinker with them.

Yet even if casual users can be convinced that they can act to protect themselves, convincing them that they need to do so remains difficult. The perception is still widespread that only crackers, stalkers, and such people need worry about loss ofSpreading clear instructions, which apparently is the next step in the FSF's campaign, seems not nearly enough -- no matter how well-crafted the instructions.

Advocating encryption, it strikes me, is comparable to being an anti-smoking activist in the 1990s: with considerable effort, you can bring about the change you advocate, but you have to be prepared for years of efforts to change people's minds. Without this effort, all the instructions in the world will not be enough to make encryption routine.


a considerable heterogeneity of histone H3 acetylation levels was observed among the same cell populations, there was no significant difference among cell types.

DISCUSSION
==========

Increased acetylation of histones may promote the overall gene transcription efficiency. In this study, we found that developing neurons in the mouse cerebral cortex and chick spinal cord exhibit considerably high level of histone H3 and H4 acetylation, compared to the proliferating stem/progenitor populations. The level of histone acetylation was reported to increase during the postnatal development of rat brain ([@B16]), consistent with our current observation. Although there was little attempt to compare histone acetylation or RNA synthesis rates between stem cells and progenitor neurons, neurons appear to have higher histone acetylation level than glial cells ([@B7]; [@B17]; [@B8]). Considering that the size of neurons greatlyThe flhDC gene affects motility and biofilm formation in Yersinia pseudotuberculosis.
The flagella master regulatory gene flhDC of Yersinia pseudotuberculosis serotype III (YPIII) was mutated by deleting the middle region and replaced by a tetracycline resistant gene, and the subsequent mutant strain named YPIIIDeltaflhDC was obtained. Swimming assay showed that the swimming motility of the mutant strain was completely abolished. The promoter region of the flagella second-class regulatory gene fliA was fused with the lux box, and was conjugated with the mutant and the parent strains respectively for the first cross. LUCY assay result demonstrated that flhDC regulated the expression of fliA in YPIII as reported in E. coli. Biofilm formation of the mutant strain on abiotic and biotic surfaces was observed and quantified. The results showed that mutation


the wrong time. Some diesel engines run leaner than gasoline engines (meaning that the air-fuel mix has a higher proportion of air than a gasoline engine). That increases the chances that the gasoline won't ignite and that unburnt fuel will be sent into the hot exhaust system--where, ironically, it could ignite, leading to possible exhaust damage. Even if you avoid that disaster, you can expect to pay $500 to drain the fuel tank, clean out the fuel lines, and refill the tank with diesel.

Some types of diesel engine use the diesel fuel as a lubricant for the fuel pump (remember, it's a fuel oil). It's said that running gasoline through such a pump could lead to serious damage or failure, turning a $500 repair into a $750-$1,250 one.

FromA Flash Insight Report

Abstract

Celent has released a new Flash Insight report titled PFM Is Dead, Long Live PFE. The report was written by Dan Latimore, Senior Vice President of Celent’s Banking practice.

Personal financial management (PFM) has been a worthy goal pursued by many providers, yet consumers continue to ignore its possibilities. Rather than trying to incrementally expand the share of 10–12% of PFM users, banks should focus on the next stage in the evolution of personal finance: personal financial experiences, or PFE.

This Flash Insight defines PFE, summarizes its promise to banks, and shows how banks can use a PFE mindset to build stronger and more profitable customer relationships.


fibroid by the hysteroscope. Hysteroscopic resections typically fall into one of two varieties. In one variety, an electrocautery device in the form of a loop-shaped cutting wire is fixedly mounted on the distal end of the hysteroscope—the combination of the hysteroscope and the electrocautery device typically referred to as a resectoscope. The transmission of electrical current to the uterus with a resectoscope is typically monopolar, and the circuit is completed by a conductive path to the power unit for the device through a conductive pad applied to the patient's skin. In this manner, tissue is removed by contacting the loop with the part of the uterus wall of interest. Examples of such devices are disclosed, for example, in U.S. Pat. No. 5,906,615, inventor Thompson, issued May 25, 1999.
Inand also grow work confidence. These compounded experiences help you to sometimes discover your inner passion from the various task performed, and also talking to peers and professionals in the same field. A youth camp volunteer wrote “I did not know I could speak in public and lead people until now. Thank you for allowing me to volunteer”. This volunteer was unaware that he was good at leading people until he volunteered as a camp worker. He has since gone to pursue a career in Human Resource Management to strengthen his abilities in motivating and leading people.

From a research standpoint, there is no statistical finding that points to the increase chances of acquiring employment through volunteering. Government, media, individuals that sponsor such idea that volunteering secures the volunteer


us to one of Linda's human grandchildren.

"She's my little clone!" Linda says of her 14-year-old granddaughter Taylor. "She rides just like me - in fact she can outride me, and she breathes horses just like I did. Her father, my son, wasn't interested in horses, so I guess it skipped a generation. Taylor lives in Ft Collins, 80 miles from me. So when I could, I used to train together with her and teach her. I started riding endurance with her and her little sister Samantha. Sammi will ride now when she has to, but she doesn't have quite the enthusiasm that Taylor has."

Taylor's first endurance ride was at age 5 in 2009, in a 25-miler aboard one of her grandmother's horses, Tupelo Honey Tu+, riding, of course,to change):

FRIDAY
Current Topics in Nutrition with Joe Pagan, PhD, of Kentucky Equine Research
Research Findings from the Virginia City 100 Mile Dehydration Study with Jerry Gillespie, DVM, AERC Research Committee Chair
Tevis Ultrasound GI Study (from 2017 ride) with Melissa Esser, DVM, MS, DACVIM, of Michigan State University
Trail Marking: Incorporating the Best from all Regions with Michelle Grald of GMHA

Wednesday, January 17, 2018

Will Connell, Director of Sport at USEF (United States Equestrian Federation), has penned a response to AERC's Letter of Concern regarding the ongoing endurance horse crisis and abuse in the some Group VII countries, namely the UAE.

While Abu Dhabi's Boudthieb Initiative has taken the lead on horse welfare, statistics venues at Dubai and Al Wathba still produce routine drug violations and catastrophic injuries in endurance races, and it


UAE National Federation, demonstrating that the FEI will take severe measures if needs must. The US Equestrian Federation has full confidence in the leadership of the FEI and supports the steps they have taken to address horse welfare in Group VII. However, US Equestrian will continue to require that at the appropriate time the FEI backs up progress reports with analytical facts.

Like the AERC, US Equestrian applauds the steps taken by HH Sheikh Sultan Bin Zayed Al Nahyan through the Bouthieb Initiative. These initiatives will contribute to the sustainability of the sport of Endurance and we would support the FEI in promoting these initiatives throughout Endurance.

Having discussed the subject with the FEI, it is clear that the UAE Federation recognises its responsibilities in managing and governing a sportwe travel, and we're gone a lot, or I'm busy, or sometimes Mike has the truck with him at the airport. It's so good things worked out the way they did."

Kristine drove to the stables and hitched up and, with the other boarders, waited to see what might happen. "It was dark now, and you could see the glow of the fire. And in my mind I was thinking, oh how horrible that Santa Paula is on fire. Turns out that town was barely touched.

"Then all of a sudden this glow got bright, within 20 minutes. It moved FAST. They say it was burning a football field every 10 seconds. 9:06 PM was a glow, and by 9:20 we were pulling out and the fire was literally coming,


after substitution with Ru and coexist with superconductivity at low concentrations of Ru. This suggests that the domains made by Cooper paired electrons are more coherent due to the high mobility of the Dirac cone states. More detailed calculations are needed to explain the electronic phase diagram of Ba(Fe$_{1-x}$Ru$_x$As)$_2$ as well as $E_{\rm F}$ and $v_{\rm F}$ of the Dirac cone states determined in the present experiments.

![(color online) (a) $B^*$ vs. $T^2$ plot for Ba(Fe$_{1-x}$Ru$_x$As)$_2$ with $x$ = 0-0.150. The solid lines were fitted using $B^*$ = (1/2e$\hbar$$v_{\rm F}$$^2$)($E_{\rm F}$ + $k_{\rm B}$$T$)$^2$. The $x$ dependence of (b) $E_{\rm F}$ and (c) $v_{\rm F}$ for $x$ = 0-0.150.](fig4){width="1.0\linewidth"}

We investigated the effect of Ru doping in iron pnictide superconductor Ba(Fe$_{1-x}$Ru$_x$As)$_2$ on MR. Linearly $B$-dependent MRs were observed below theis unprecedented in NHS history. Professor John Appleby, the chief economist at the Kings Fund, a UK health policy think tank, stated that standards within the NHS were slipping at rates worse than the early 90’s – an apparently notorious low point in NHS history. [10] A report mandated last year by the British Parliament noted, amongst other things, that: waiting time targets for A&E, hospital treatment and cancer care were all being missed; bed occupancy was increasing to “very high levels”; and delays in discharging patients were significantly rising. [11]

The South African economy is already under tremendous pressure – it is unlikely it could cope with a public sector drain comparable to that of the NHS this year. Because of this, standards will slip even more than


About us

GoaKaido.com is an initiative of N.D. Agrawal and Narayani Publications. This online service aims to provide latest updates in the State laws, which may include amendments or new legislations. This is a novel and unique resource, which promises to be of great assistance to all the legal stakeholders, due to its ready availability and ease of use.

Our features: 1. GoaKaido.com is a disabled-friendly website2. It is mobile-device compatible.3. All the registered members of GoaKaido.com will get email alerts as soon as an update/amendment/new legislation is made available on the website.

4. Mobile app.

Late Smt. Narayani Agrawal

Narayani Publications is a proprietary firm established by Smt. Rajkumari Agrawal in 2013 in the memory of her sister-in-law Smt. Narayani Agrawal with the aim of providing quality legal resources in form ofdid not change, with no decrease in analgesic prescriptions; for 14% of the patients analgesic prescriptions increased. In the intervention group, analgesic prescriptions changed for 25% of the patients, decreasing for 5% and increasing for 20%. A decrease in the incidence of pain described as more than life's usual aches and pains was found for the intervention group (p = .05). No significant difference was found between the groups for the patients undertreated for pain, as measured by PMIs. Although analgesic regimens were altered significantly when the physicians understood more about the patient's pain, cancer pain management remains a complex problem. Future studies should focus on the long-term systematic incorporation of simple pain-assessment tools into daily outpatient oncology practices as well as on innovative ways to address other


universities, faculties and clinics. Thus they are not available very easily. If there exist some survey works, they are mostly conceived on the national level. The objective of the study presented here is to show a comparatively comprehensive survey dealing with the medical student instruction on the international level, analyze trends in development and identify problems that have not been solved so far. The work consists of five chapters. The first one gives a short survey of current concepts in communication with patients reaching beyond the traditional training in taking medical history. Among others, the following factors are included here: explanation of both diagnostical and therapeutical methods to the patient, informing the patient about results obtained from the examination, telling him or her general conclusions and recommendations, informingGaudi, Gaudi Everywhere! by:Beatriz Lopez

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Each major city boasts a unique cultural icon that defines and shapes its image

. Think of New York City and the Empire State Building comes immediately to mind. Picture Paris and the elegant curves of the Eiffel Tower springs into view. Sydney has its Opera House and London, the clock face of Big Ben. When it comes to Barcelona what other building but the Sagrada Familia encapsulates the spirit of this modern, laidback city so precisely?

Barcelona is imprinted with the genius, spirit and architecture of its most famous son - Antoni Gaudi. His most well known work is the ongoing construction of the Sagrada, but if you look closely enough his mark is everywhere in Barcelona, on flowery pavement tiles and ghostlike balconies, in


2 charge.

Casa Batllo

The House of Bones, as this structure is known, has creamy grey skull shaped balconies dotted across its faade. Formerly a private residence, the insides of Casa Batllo is covered in iridescent cerulean, navy and cobalt tiles. Gaudi drew inspiration from the sea for this house, swirling the bony spine of a whale, undulating waves of water, fish gills and the graduated blues of the ocean into a staircase, the ceiling, windows and atrium of Casa Batllo. The rooftop represents St George and the dragon, with its twisting, dragon-claw chimneys and undulating dragon back, scaled in shiny tiles of red, green and yellow.

Casa Batllo is open from 9am to 8pm on most days, and the entrance fee of 16.50 includes an audio guide, helpful to gainingpreserved between the source of the Vistula and the middle basin of the Dnieper. Its northern regions adjoin territory where river names of Baltic origin (Daugava, Neman and others) abound. On the south and east, it borders the area of Iranian river names (including the Dniester, the Dnieper and the Don). A connection between Proto-Slavic and Iranian languages is also demonstrated by the earliest layer of loanwords in the former; the Proto-Slavic words for god (*bogъ), demon (*divъ), house (*xata), axe (*toporъ) and dog (*sobaka) are of Scythian origin. The Iranian dialects of the Scythians and Sarmatians influenced Slavic vocabulary during the millennium-long contact between them and early Proto-Slavic.

A longer, more intensive connection between Proto-Slavic and the Germanic languages can be assumed from the number of Germanic loanwords,


shoulder shirts - I had been looking at these for ages and they were never on sale! They were originally priced at $22.50, and then appeared in clearance at $7.49. I would have preferred the dark gray in XS, but they only had S, but they did have the XS in light gray. I tried them both on and they both looked good, I couldn't make up my mind so I got both!

Rosette shirt - I had never seen this in the store before, but it was originally priced $17.50. It's so cute! They also had a peach color but it looked bad with my skin tone. I wear dark colors a lot more anyway. $5.99

Turtlenecks - I had bought two around October when they were $8 agood relations with the Pentagon, in recent years it has come to symbolize many of the procurement problems that beset the Defense Department.

In the late 1970s, Adm. Hyman Rickover, then head of the Navy's submarine force, charged that General Dynamics was artificially inflating the costs of attack subs. The investigations and lawsuits sparked by this claim have yet to be resolved.

Ironically, last year the Navy concluded that General Dynamics had improperly given Admiral Rickover some $67,000 in gifts over the course of their relationship.

In the fall of 1983, P. Takis Veliotis, head of General Dynamics' marine division, was indicted for allegedly receiving kickbacks on ship contracts. Mr. Veliotis fled the US, claiming he was a bystander to a companywide sub fraud effort.

Last October, George Sawyer, General Dynamics vice-president


Break The Rules - Why It's Okay To Be Faking It

I was having a (gasp!) coffee with a friend of mine recently and we started talking about what some of the yoga practices were about. Newer to yoga, my friend noticed that she felt confused by some of the dogma she's come into contact with. She noticed that sometimes practices seem artificial or downright judgmental. She didn't like to sing what she didn't understand. And, she'd long ago decided that self-worth was tied to lifestyle choices. She came to yoga as a way for to connect, not a way to classify.

I told her I felt like that too.

Who Made The Rules

Having been weaned on rigidity, I instantly understood what she meant. For me, rules and guidelines have oftenbeen scapegoats for deeper discontent, and sometimes rules have been downright harmful. I've learned (through many missteps) that I need to question- WHO made the rules. I need to respect my own needs over deliciously tempting dogma. I need to stay connected with change, not become attached to control. I need to stay in the slippery mental space where I am most alive. And, for me, this is what my yoga practice is about.

I know I still need to struggle and watch my mind flirt with rigidity. I know I need to embody change until it doesn’t scare me. I know I need to connect to what is real and visceral for me, even when it hurts. I know I practice so that I can practice settling into


a way of distinguishing sister from homologous chromosomes," Baumann says. How do they do it? That's something the group is now investigating.

Another big unknown is precisely how the lizards end up with double the amount of chromosomes in the first place. Baumann suspects that it could happen over two rounds of replication or if two sex cells combine forces before the division process starts.

Although asexual reproduction might seem like a bore—and one that can have questionable genetic outcomes unless done right—it has its benefits, too, Baumann notes. "You're greatly increasing the chances of populating a new habitat if it only takes one individual," he says, citing the example of the brahminy blind snake (Ramphotyphlops braminus), another parthenogenetic species. "If she has a way of reproducing without the helpof the foetus.

Erekson said that earlier reports tend to support that the foetus does well under anaesthesia.

Ecker said he'd like to see larger studies that track the outcomes of babies whose mothers had surgery while pregnant.

Health24 is South Africa's premier health information website

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Jesus prayed and through his prayer life taught us invaluable principles and model for praying. It is some of this principles that we will explore in this discourse. There are only 17 references to Jesus praying and most of them are in the book of Luke. We will examine some of them and the principles that they expressed. It is the power combo of prayer and fasting that ultimately changes our spiritual status and levels! It is therefore important to understand the principles that makes both work! So here we study the principles for effective praying.

"And it came to pass, that, as he was praying in a certain place, when he ceased, one of his disciples said unto him, Lord, teach us to pray, as John also taughtQ:

Python-pandas - Datetimeindex: What is the mosty pythonic strategy to analyse rolling with steps? (e.g. certain hours for each day)

I am working on a data frame with DateTimeIndex of hourly temperature data spanning a couple of years. I want to add a column with the minimum temperature between 20:00 of a day and 8:00 of the following day. Daytime temperatures - from 8:00 to 20:00 - are not of interest. The result can either be at the same hourly resolution of the original data or be resampled to days.
I have researched a number of strategies to solve this, but am unsure about the most efficienct (in terms of primarily coding efficiency and secondary computing efficiency) respectively pythonic way to do this. Some of the possibilities I have come


big part of why I still play and love the game, I’m eager to see future community events. I wonder how we’ll see the racing meta evolve as everyone's figuring out new ways to play the game.

Sponskapatrick:

First off, I'm a huge fan of the base game... with roughly +650 hours into the game :P

Anyway, hearing first of Amplified got my HYPED, but also just a tiny bit worrysome... Like what would happen if certain items got removed/changed. Will Z5 be waaay too hard ?

Nocturna is one of the new available characters featured in the DLC, AND OH MAN, is she fun to play Well... only that she might be a little bit too easy, as her Cutlass pretty much gives her infinite invincibility if used correctly. Still reallyprotagonist. Amplified added a new remix, by OverClocked Remix, additional remixes by the original cast of Necrodancer musicians, and 20 new tracks to account for the new Depth 5 and Boss Fortissimole. I sway more towards the FamilyJules soundtrack, but the variety that these remixes offers is continual variations each time the game is played. I’ll forgot about a certain remix, hear it during a run, and be instantly brought back into the magic of the game.

Speaking of magic, the new items bring a new sense of wonder to the game. The combination with pre-existing items itches my sense of exploration and intrigue. I’m constantly exclaiming “For Science” during my runs, just to figure out how everything interacts. A new depth, enemies, and boss are icing on the


dozen cars of which no more than two are likely running at any one time. They are good, solid working class folks and probably the best neighbors I’ve had.

Miss J’s health has not been good. She needed heart bypass surgery a few years back and had problems with congestive heart failure. She also has emphysema. Over the winter, I didn’t see much of her. When I did see her, I noticed that her breath was short and her face looked puffy. She told me the emphysema was getting worse and she was on prednisone and home oxygen. She appeared to be wasting away. Soon she was in the hospital for her breathing and congestive heart failure. She made it home briefly but was frequently back in the hospital.of her if she comes home. I’m broke and I owe these people so much money that I’ll die broke, doc.”

And this is the reality of our healthcare system. Not only will Miss J die, but her terminal illness will leave her widowed husband in financial ruin. What option does he have? Say no to dialysis? Just bring her home and let her die? He’s faced with the choice of being either financially or morally bankrupt. And no one should have to make that choice.

And so as Miss J dies a little every day, her husband does too, faced with both a life without his wife and insurmountable debt and financial hopelessness. Welcome to healthcare in America.

This broken “system” causes incredible suffering and needs to change. Coloradans have


good 'what are you doing?'. For example, an inspirational quote or something funny is good. Try adding ♥s by holding alt and 3 (pgdn) to separate phrases.

7

Have fun! Nobody likes a bore, so lighten the mood and you'll get buddies in no time!

Part 3

Befriending others

1

Add people! Not everyone is confident enough to start a conversation and make friends, so be the one to reach out first.

2

If you have one friend, level 5 or over, they will be a great help now.

3

If you don't have a friend level 5 or over, add one. It will help this process a lot.

4

Click your friend. Look where their best friends are, and where their boyfriend is.

5

Click the boyfriend, and click add. Click one of the best friends, click add. Click another bestmask) is not a very good identifier for a device because after disconnecting, another device can assume that subnet mask. F*ck.
3.3: Use short-lived JWTs. A bit of a hacky solution imo. We set the JWT exp to 15-30 min and assume that in that time, an attacker can't cause much damage. Critical operations like deleting an account should still require password (which will be sent over https), limiting the scope of the attack. After 15 min, the user will be prompted to log back in and can revert all the effects or contact support to remove them.
However, a new problem arises: we don't want users to have to login every 15 min. This is where my understanding ends:
3.3.1: Use a long-lived refresh token that is stored as a


indeed.
They explain a "nice" 'trick'.

Two's compelment overflow detection can be achieved by XORing the carry-in and the carry-out bo=its of the leftmost full adder.

Their diagram. See above ref for detailed comment:

thrust upon the victim (quite literally) or terminating it, she should be well within her rights to end it.

Morgan:"There will be people in that situation (of having a raped daughter who gets pregnant) and they're looking at their daughters and saying, 'If I make her have this baby, isn't it going to ruin her life?'"Santorum: "Well, uh, you could make the argument that if she doesn't have this baby, if she, if she, kills her child, that that too could ruin her life...(she should) accept this horribly created...gift."

Did you get that? "Accept that horribly created gift." If I may be so bold as to quote loosely from my linked post from 2006 above and adapt it to Santorum:

(Imagine) Some dickless piece of shit drags her (your daughter) into


an alley and punches her around a bit, then sticks his Keebler Elf-sized dick in her and happens to get her pregnant. You expect her to keep that kid?

How about this: we send you (Santorum) to prison where we allow the Aryan Brotherhood to rape you in the shower. Afterwards, you and your bleeding asshole are going to be dragged to a cell where some guy will tattoo a picture of a fetus on your stomach in bright red ink. Now, do you think you should be allowed to go and get that tattoo laser-removed? I don't. Nope - your life is not in danger now, is it? You should have to respect that piece of art for the rest of your life. It's art, Rick! Come on!and state of enormous proportions" because of Obama's not supporting a national ban on gay marriage. As Dr. Evil once said, "Oooooooo...I'm shaking in my little space booties."

Ratz continued by saying:

"The family unit is fundamental for the educational process and for the development both of individuals and states; hence there is a need for policies which promote the family and aid social cohesion and dialogue..."

I'd like to know what evidence Ratz has for gay marriage being bad in any way. Where are the countries that have collapsed because of legalized gay marriage? Hell, I live in Canada and we've had legalized gay marriage since 2005 - where is the societal downfall? What about the Netherlands? They've had it since 2001 and they seem to be doing just fine.


whether or not the real estate taxes were paid and further providing that only a deed or patent was required to carry thereon the County Auditor's certificate relative to taxes. Therefore it is logical to assume that there was no record made of the transfer in the office of the County Auditor at the time the final decree of distribution was placed on record. When the certificate of the Register of Deeds was obtained by the County Auditor on October 22, 1938, as stated above, it was either misinterpreted or not followed by that office in the preparation of the notice of the expiration of the period of redemption. It is apparent from the instrument itself that the *358 Register of Deeds did in fact state thereon theabsence of showing that the owners were misled thereby was of no effect. Buman v. Sturn, 73 N.D. 561, 16 N.W.2d 837.
This Court has held on many occasions that a tax deed issued without strict compliance with the statutory requirements as to the service of the notice of the expiration of the period of redemption is void. Messer v. Henlein, 72 N.D. 63, 4 N.W.2d 587; Blakemore v. Cooper, 15 N.D. 5, 106 N.W. 566, 4 L.R.A.,N.S., 1074, 125 Am.St. Rep. 574; Davidson v. Kepner, 37 N.D. 198, 163 N.W. 831; Baird v. Zahl, 58 N.D. 388, 226 N.W. 549; Biberdorf v. Juhnke, 59 N.D. 1, 228 N.W. 233.
In Cruser v. Williams, 13 N.D. 284, 100 N.W. 721, this Court said:
"The redemption period does not terminate, or the


a lapse of time and by the statute of limitations which had run for more than ten years prior to the commencement of this action and further that they, the plaintiffs, are guilty of laches. The trial court held that the residuary statute of limitations, Section 28-0122 of the NDRC of 1943, is applicable and that the defendant, George Weisz, and his grantor Williams County had held the land adversely for more than ten years before the commencement of this *359 action, and the plaintiffs' cause of action had accrued more than ten years before action was commenced, and the plaintiffs were therefore barred from asserting their claims against the defendant, George Weisz. With this we cannot agree. Williams County took tax deed on March 1, 1940 andfor iPad app.

NBC has also updated its iPad app with the same content found on the NBC.com web site. Yes, you can finally stream The Office to your iPad! Also, their TBS for iPad program does the same for Turner’s other shows, including episodes of Conan, Tyler Perry’s House of Payne, available free on your tablet.

It would appear that NFL Sunday ticket is coming to AppleTV, Boxee and Roku players if a survey dug up by Engadget has any merit. Not surprising as it is a natural extension of the AppleTV experience. But will it be delivered as an app or as a built in module in the way Netflix is currently delivered. Notice there is no mention of GoogleTV? expand full story

If you love the smell of


can also enjoy Internet radio, allowing them to browse and listen to thousands of Internet radio stations, as well as tag favorite stations to listen to later. Apple TV’s support of HD photos is enhanced with iPhoto Events, which simplifies finding your favorite photos on Apple TV, as well as iPhoto® Faces, which gives access to photos organized by people identified in iPhoto.

Apple TV users have direct access to a catalog of over 8,000 Hollywood films on iTunes including over 2,000 in stunning HD video available for rent or purchase. Users can also choose from a selection of 11 million songs, 10,000 music videos and over 50,000 TV episodes to purchase directly from their Apple TV or browse and enjoy the iTunes Store podcast directory of over 175,000TV. Video availability varies by country.

Apple ignited the personal computer revolution in the 1970s with the Apple II and reinvented the personal computer in the 1980s with the Macintosh. Today, Apple continues to lead the industry in innovation with its award-winning computers, OS X operating system and iLife and professional applications. Apple is also spearheading the digital media revolution with its iPod portable music and video players and iTunes online store, and has entered the mobile phone market with its revolutionary iPhone.

Today’s new EULA for iTunes throws up a pretty obvious clue that an AppleTV 3.0 update is coming up – perhaps in a matter of days. The neglected hobby of Apple’s isn’t dead yet folks. Besides iTunesLP, is there any possibility of something useful like Hulu or


FreeAgent Theater+ HD media player leverages a docking system unique to Seagate FreeAgent Go™ portable drives and accessories, providing a quick and easy way to connect your external hard drive to your television or home entertainment center. After loading the drive with your favorite movies, videos, music and pictures from the home PC, simply slide it into the FreeAgent Theater+ dock to get instant access to your digital media library. With the FreeAgent Theater+ player, you can just sit back, relax, point, click and enjoy as digitally captured memories and your favorite movies come to life in 1080p HD video, DTS™ 2.0 + Digital Out, or Dolby Digital technology. The interface is easily navigated with the included remote control, and you can choose from video, photos or musicvideo below to find out and don't miss our Dodge Dart First Drive right here!

As someone who has seen numerous Dodge Dart models starting 50 years ago, I think this is the BEST of the small Dodge Darts. When they were mid-sized to large, they were stylish, V8, coupes, long, sexy...but for today's downsized, less flamboyant styles, this is much, much better than the bland Darts of the mid-60s.

the size reminds me of the old Mazda6 before it went jumbo.. was a nice size for many buyers... not too big, not too small but sporty.. but the problem was they essentially charged the same price as the bigger midsizers, so sales were limited. Not the case here... Looks like Dodge is not making the same mistake.

I wonder what


kind of performance the 1.4T will put out in the relatively portly Dart. The Abarth performance is not bad. Low 7's in stick and mid to high 7's with slushbox? That's more than enough fun, especially if it comes with nice feeling handling and around 40 MPG.
Now, Dodge, how about an American wagon or hatch of this?

Sorry the Goob couldnt watch this whole video, it was too painful.
Last week on the Jessi podcast, Jessi reviewed a hybrid Acura.
This week, she's prattling on and on and on about hybrid Dodge minivans.
This makes the Goob sad, and very sleepy.

in R/T form with the 2.4L/6spd it's not a bad alternative for a mid-size... it is essentially a small mid-size.
It's about the same price of an entry jumbo midsizers, but has leathergrowth later in the period that contributed to yuan weakness. Signs of slowing momentum in the domestic economy were exacerbated by deterioration in the outlook for global trade.

Global bonds

Global bond markets suffered from bouts of volatility in Q2 due to a confluence of factors. These included a greater dispersion between accelerating US growth and a softening of economic activity elsewhere, escalating trade tensions between the US and China and the formation of a populist coalition government in Italy.

US 10-year Treasury yields rose from 2.74% to 2.86%. They rose significantly in April, touching a seven-year high in mid-May, as growth and inflation expectations continued to build, before risk aversion and “safe haven” buying led to a significant retracement. Bund 10-year yields fell from 0.50% to 0.30% on safe haven


DESCRIPTION:

18 year old babe Liza Rowe is ready to get on her knees and spread her ass for All Anal All The Time! See the full video by clicking on the banner below!

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Show lesshelp monitor the activities of the numerous armed groups active in DRC. If there have been no additions to the proposed force, then this reduced the MONUSCO fleet to four unmanned aircraft. Later reports indicated that the drone’s surveillance equipment was undamaged and repairs would allow the aircraft to return to operations.

Concerns about the basic safety of drones have been a matter for debate domestically in both the US and Europe, and were also said to have been the primary motivator for moving US drone operations in Djibouti from Camp Lemonnier to a remote desert airstrip, Chabelley Airfield, last September. The issue of basic safety in these operations will no doubt become a matter of debate in this instance as well.

A team of technicians prepare a Falco unmanned


M23 representatives reiterated the end of the movement as an armed group. No amnesty for those believed to be responsible for war crimes was included. DRC President Joseph Kabila was said to have been hailed by other African leaders present at the ceremony for signing the DRC’s document. The US and UN also responded positively to the deal.

As always it remains to be seen how this new agreement will play out. As previously noted here and elsewhere, M23 was only the latest iteration of Tutsi rebels in the region. There is nothing to say that grievances, real or imagined, of government inattention or outright hostility to the Tutsi minority in eastern DRC might not again provoke a return to open conflict. With the status of former M23 membersto seek antenatal care later and fail to stay in regular contact with health services during pregnancy \[[@pone.0182544.ref004]\]. The differences in health behaviors and outcomes between socially advantaged and disadvantaged women and children are mediated by a number of factors including lack of social support \[[@pone.0182544.ref005],[@pone.0182544.ref006]\], lack of education \[[@pone.0182544.ref007],[@pone.0182544.ref008]\] and poor living environment \[[@pone.0182544.ref009]\].

Inequalities in health are not limited to physical functioning; for example women with higher socioeconomic status have lower rates of depression \[[@pone.0182544.ref010]\]. Postpartum depression (PPD) is characterized by low maternal mood within the year after childbirth \[[@pone.0182544.ref011]\] and is associated with reduced sensitivity in interaction and emotional withdrawal \[[@pone.0182544.ref012],[@pone.0182544.ref013]\]. The effects on child development include adverse child physical, social and emotional developmental outcomes such as poor cognitive development and greater likelihood of insecure attachment


370.  She argued that although Lorin Duemeland executed the will, he “really didn’t mean it; he was merely bluffing.”
Id.
  On appeal, we upheld the will, concluding because Norback conceded the will was properly executed and unambiguous, Lorin Duemeland’s testamentary intent must be derived from the will and not from extrinsic evidence.
Id.
 at 371.  We said the consequence of Lorin Duemeland executing an unambiguous will was that it had a legal effect on his death, and it was irrelevant whether he harbored a secret intention that his actions would have no effect on the disposition of his estate.
Id.

[¶7]	Olga Duemeland died on April 27, 2001.  Although this record does not include an inventory of the assets of the Trust Estate, the parties apparently
 agree that whenpower of appointment whether or not he survived her.  George Duemeland argues the term “Family Trust” describes Olga Duemeland’s existing “Trust Estate” at her death, which the Trust Agreement thereafter referred to as the Family Trust for convenience.  George Duemeland argues Lorin Duemeland’s exercise of the power of appointment before Olga Duemeland’s death was not invalid merely because the assets could not be distributed until Olga Duemeland’s death.

[¶21]	We conclude both parties have made rational arguments for different interpretations of the Trust Agreement and Lorin Duemeland’s will.  Paragraph 8.1 of the Trust Agreement authorizes the Trustees to distribute “[w]hatever remains of the Trust Estate after the Settlor’s death and after complying with the foregoing terms and provisions of this Trust Agreement (hereinafter for convenience called the ‘FAMILY TRUST’) .


. . as hereinafter set forth.”  Paragraph 8.3 applies “[u]pon the death of the Settlor’s husband (or upon the death of the Settlor if her husband does not survive her)” and authorizes the Trustees to “distribute whatever then constitutes the FAMILY TRUST” in amounts and upon terms as Lorin Duemeland shall appoint “by a will referring specifically to the power herein granted to him.”  Lorin Duemeland’s will identified paragraph 8.3 of the Trust Agreement creating the Olga Duemeland Irrevocable Trust and directed the “principal and undistributed income of the FAMILY TRUST remaining at my death be paid over and distributed” to George Duemeland.  When read as a whole, those provisions reasonably can be construed to authorize Lorin Duemeland to exercise the power of appointment if he predeceased Olgaread Nancy’s story here.) We chose Dr. Stephen Hufford’s patient rooms as our first RTR4C project after witnessing their dismal state. In his defense, Dr. Hufford had just returned to work after a difficult year battling pancreatic cancer. His chances of recovery were slim. He would live just 17 more months — enough time to see the wonderfully transformed space and to host a reception to show his gratitude for our RTR4C volunteer designers. The reception was held in September 2012. Dr. Hufford passed away a month later. Dr. Kevin Knopf has since taken over the practice and is committed to maintaining the new look and feel of the treatment rooms. Six different volunteer designers renovated the seven patient rooms in Dr. Hufford’s office, creating unique environments they


the government and the patriot movement, he wrote the following to Gov. Francis Legge:

Proposed to raise a Battalion of Light infantry or Royal Fensible Americans, To consist of Five Companies.
	1 Lieut. Col. Commandant and Captain,
	4 Captains,
	1 Capt. Lieut.,
	4 Lieutenants,
	5 2d Lieutenants or Ensigns,
        15 Serjeants,
        15 Corporals,
        5 Drummers,
 	300 Privates,
	1 Adjutant,
	1 Surgeon,
	1 Surgeon's Mate.
That they be clothed and armed as Light Troops and put on the same Establishment of Pay as the late 80th, Gage's Light Infantry and [this] Corps to be employed on the like Services.

"Fencible" troops differed from militia in that they were full-time soldiers and were paid; however they differed from regulars inregion arrived in Halifax, prompting the authorities to dispatch Goreham's regiment to Fort Cumberland in late May, first overland to Windsor and then by ship via Minas Basin and Chignecto Bay.

Fort Cumberland (originally built by the French as Fort Beauséjour in 1750) was in an advanced state of disrepair by 1776. Goreham set his men to reconstruction, both the buildings and the earthworks, but work went slowly due to the shortage of supplies and equipment, and lack of assistance from the local population, who were mostly pro-patriot. With winter approaching, Goreham had his men put more effort into the accommodations than the fortifications. In spite of this, the fort would be a hard nut to crack by any rebel force without artillery.

Battle of Fort Cumberland

The fort was


ship lying in the mud at low tides.

Eddy's army staged its next coup on the night of Nov. 6/7 when a party of 30 men, led by Zebulon Roe, set out to capture the Polly. After a difficult approach march over the tidal flats, with the tide rising, Roe's men took the RFA party aboard by surprise and captured the sloop without firing a shot. Waiting for the rising tide, Roe's men captured a work team and several officers coming to the ship. Finally as the fog lifted around 7am, the rebels were able to sail and tow Polly out of the creek, followed by cannon fire from the fort, which fell short. In all, Goreham lost 49 officers and men captured. Added to the men lost atlittle

explanation, the District Court denied Hardwick’s motion on the sole ground that the

of the Fair Sentencing Act . . . were in effect at the time the covered offense was
committed.” First Step Act of 2018, § 404(b), 132 Stat. at 5222. Section 404 further
defines “covered offense” in relevant part as “a violation of a Federal criminal statute, the
statutory penalties for which were modified by section 2 or 3 of the Fair Sentencing
Act[.]” Id., § 404(a). Section 2 of the Fair Sentencing Act is the section that increased
from 50 grams to 280 grams the quantity of crack cocaine necessary to trigger the
sentencing range of 10 years to life under which Hardwick was sentenced. See Fair
Sentencing Act of 2010, § 2(a)(1), 124 Stat. at 2372.


to violate §§ 841(a)(1) and 841(b)(1)(A). Although a life

sentence was statutorily authorized when the District Court sentenced Hardwick, the Fair

Sentencing Act has since reduced the statutory penalty for crack cocaine offenses like his

to a term of five to 40 years. Hardwick’s motion under the First Step Act required the

District Court to assess his request for a sentence reduction in light of that change to the

statutory scheme. See Beamus, 943 F.3d at 791-92; Wirsing, 943 F.3d at 185;

McDonald, 944 F.3d at 772.

       The District Court, however, focused solely on Hardwick’s offense level under the

Sentencing Guidelines, which was relevant to Hardwick’s Guidelines range and which

the Fair Sentencing Act did not change. Whether a defendant’s Guidelines range has

changed is a determinative factor in adjudicating motionscore, the paper tube wound on the core is separated from the core and discharged in the longitudinal direction of the core if a friction force generated between the rollers and an outside surface of the paper tube is greater than that generated between the core and an inside surface of the paper tube.
According to a method for manufacturing a quadrangularly wrapped paper tube disclosed in U.S. Pat. No. 4,120,523, a quadrangularly wrapped paper tube is manufactured by successively forming a circularly wrapped paper tube, which is continuously formed and discharged, to have a quadrangular cross section using a plurality of forming rollers.
The foregoing discussion is to provide general background information, and does not constitute an admission of prior art.


inputs, final_endpoint=endpoint)
        self.assertTrue(out_tensor.op.name.startswith(
            'InceptionV1/' + endpoint))
        self.assertItemsEqual(endpoints[:index+1], end_points)

  def testBuildAndCheckAllEndPointsUptoMixed5c(self):
    batch_size = 5
    height, width = 224, 224

    inputs = tf.random_uniform((batch_size, height, width, 3))
    _, end_points = inception.inception_v1_base(inputs,
                                                final_endpoint='Mixed_5c')
    endpoints_shapes = {'Conv2d_1a_7x7': [5, 112, 112, 64],
    push the country farther from its postwar pacifism: adding a more patriotic tone to Japan’s school textbooks. . . .

. . . . Mr. Abe and the nationalists have long argued that changes in the education system are crucial to restoring the country’s sense of self, eroded over decades when children were taught what they call an overly negative view of Japan’s wartime behavior.

The latest efforts for change started slowly, but have picked up speed in recent weeks.

In October, Mr. Abe’s education minister ordered the school board here in Taketomi to use a conservative textbook it had rejected, the first time the national government has issued such a demand. In November, the Education Ministry proposed new textbook screening standards, considered likely to be adopted, that would require the


that argues for the elimination of privileges extended to foreigners who had been granted Special Foreign Resident status—mostly Korean-Japanese.

The Zaitokukai also collect a lot of money in donations from like-minded citizens.

The group, which is led by Makoto Sakurai, whose real name is Makoto Takada, claims that ethnic Koreans abuse the social and welfare system in Japan. Zaitokukai claims to have over 14,000 members. It organizes protests and demonstrations across Japan, even in front of Korean elementary schools, yelling such slogans as “Go back to Korea,” “You’re the children of spies”—making numerous veiled and overt threats. The group asserts that all foreigners are criminals who should be chased out of Japan, especially the Koreans.

In a recent book, Sakurai states, “The Japanese understand what the Koreans are up to. Ifthe terminology of a true believer, inadvertently using the words “Zainichi Tokken (Special rights of the Korean Residents In Japan)” herself. At a press conference held today (September 25th), she was questioned about her use of the term and stated uncomfortably, “In my reply (to TBS) I might have just copy and pasted from the Zaitokukai homepage.” She refused to criticize the group by name or clarify whether she believed that ethnic Koreans had special privileges.

Yamatani, in her current position, oversees the National Police Agency—the very same agency that noted in its 2013 white paper that the Zaitokukai were committing hate speech, promoting racism, and posed a threat to the social order. If hate-speech becomes a crime, she may be in charge of overseeing the police that enforce


under Japanese imperial aggression during World War II, a conflict in which Japan was allied with Nazi Germany. . . .

7. Abe cabinet ministers Tomomi Inada and Sanae Takaichi wrote promotional blurbs for a book written by a Liberal Democratic Party politician that formally articulates the strategy endorsed by Taro Aso.

An official of the Liberal Democratic Party urges in a new book that his party try to regain power by adopting a new role model: Hitler.

The book, “Hitler Election Strategy: A Bible for Certain Victory in Modern Elections,” says the Nazi leader’s process for “unifying public opinion in a short period of time and snatching power” provides “very important teachings.”

The author, Yoshio Ogai, is a public relations official in the Tokyo chapter of the Liberal Democratic Party, Japan’sresolved with liberal policies,” he said.

“Abe is expected to reappoint a broadly similar Cabinet after he is formally named prime minister again by the Lower House on Dec. 24.” Ummm…

Is ISIS’s hostage-taking going to be the final catalyst for Japan’s shedding of its pacifist constitution? It’s possible:

The Daily BeastISIS Pisses Off Pacifist Japan by Taking Hostages
Tokyo is unlikely to pay a $200 million ransom to free two hostages. If they die, the country’s anti-war constitution may go with them.

Jake Adelstein
01.20.15

TOKYO — Last week, Prime Minister Shinzo Abe pledged $200 million in non-military assistance to support countries affected by the campaign against ISIS during an ongoing six-day Middle East tour. Today (January 20th Japan time), The Islamic State released a video threatening to kill two Japanese hostages unless they


127 million – “at around 100 million”.

…

Less than 2pc of the population is foreign, and that includes hundreds of thousands of long-term residents from China and Korea

Japan ministers go to Yasukuni a day after China talks
By Miwa Suzuki
April 23, 2015 6:46 AM

The day after Japan’s Prime Minister Shinzo Abe sat down for his first substantial talks with China’s Xi Jinping, three of his cabinet ministers Thursday visited the war shrine Beijing sees as a symbol of Tokyo’s violent past.

Visits by the three women have the potential to muddy diplomatic waters that were starting to clear after their nationalist boss sat down with the Chinese president on the sidelines of a regional summit in Jakarta.

“I offered my sincere appreciation for the people who fought and sacrificed their precious livesand current territorial disputes.

Abe later told reporters that they had a “very meaningful summit meeting” and bilateral relations were improving.

In Tokyo Thursday, Chief Cabinet Secretary Yoshihide Suga, speaking after Yamatani’s pilgrimage, said it should have no bearing on warming China ties.

“I don’t think there will be (any impact). The visit was made in a personal capacity.”

– ‘Unlikely to damage’ –

Masaru Ikei, professor emeritus at Keio University and an expert on Japanese diplomatic history, said shrine visits like this were somewhat inevitable, but unlikely to be a disaster.

“It would have been better if cabinet ministers had stayed away, as well as the prime minister,” he said.

But Abe could not stop ministers from going “in a private capacity”, he said, pointing to the political need for conservative politicians to appease


by order of officials in the city of Sagamihara, where he lived and where the residence for disabled people, Tsukui Yamayuri-en, is located.

In addition to writing the letter, he had told co-workers at the facility, home to about 150 people with mental and physical disabilities, that he thought severely handicapped people should be euthanized, the center’s management said.

Mr. Uematsu spent two weeks in the hospital before two doctors determined that his psychotic symptoms had abated and that it was safe to release him, the authorities said. He was released into the custody of his parents and was supposed to return to the hospital for outpatient treatment, but it appears there was little follow-up.

“There’s nothing in the law that specifies what the city is supposed to do after release,”retain his righthand man, Chief Cabinet Secretary Yoshihide Suga, along with Finance Minister Taro Aso and Foreign Minister Fumio Kishida.

Economics Minister Nobuteru Ishihara will also be kept on, along with Health, Welfare and Labour Minister Yasuhisa Shiozaki. Deputy Chief Cabinet Secretary Hiroshige Seko will become trade and industry minister.

Tamayo Marukawa, the environment minister in the previous cabinet, was appointed minister to oversee preparations for Tokyo’s 2020 Summer Olympic Games.

Abe also appointed a new LDP executive line-up.

The appointment of Toshihiro Nikai, 77, as LDP secretary general was seen as signaling Abe’s hopes for a third term.

…

“She also regularly visits Tokyo’s Yasukuni Shrine for war dead, which China and South Korea see as a symbol of Japan’s past militarism. Japan’s ties with China and South Korea have been frayed by


Safety Commissioner socializing with members of the country’s most virulent racist group, Zaitokukai, were brought to light in an expose by Japan’s leading weekly magazine, Shukan Bunshun. In U.S. terms, it would be the equivalent of the attorney general getting caught chumming around with a Grand Dragon of the Ku Klux Klan. This week it was reported that another cabinet member received donations from them, and that Prime Minister Shinzo Abe himself may have ties to the staunchly anti-Korean organization.

The standard line of defense offered by the cabinet members embroiled in controversy over their connections to racist groups, “We just happened to get photographed with these people. We don’t know who they are,” is getting harder to swallow. And it has raised some disturbing issues.

…

The Zaitokukai, founded infor over a decade. When replying to questions from TBS radio about the recent scandal, she explained the Zaitokukai exactly in the terminology of a true believer, inadvertently using the words “Zainichi Tokken (Special rights of the Korean Residents In Japan)” herself. At a press conference held today (September 25th), she was questioned about her use of the term and stated uncomfortably, “In my reply (to TBS) I might have just copy and pasted from the Zaitokukai homepage.” She refused to criticize the group by name or clarify whether she believed that ethnic Koreans had special privileges.

Yamatani, in her current position, oversees the National Police Agency—the very same agency that noted in its 2013 white paper that the Zaitokukai were committing hate speech, promoting racism, and posed a


says is correct,” said the acting head of Nippon Kaigi, Tadae Takubo, in a press conference last month.

This seemingly benign statement puts Abe and his cabinet in a difficult position. If they really wish a return to the Emperor as the center of the government, and believe his words are sacred—they will have to obey them. They will have to let him retire and respect his wishes for a pacifist Japan, and a constitution that guarantees basic human rights and renounces war.

It may be a bitter pill to swallow, but such would be the word of a god. Even a retired one.

“Monday’s speech left little doubt his desire and intent is to step down, if such a thing can be arranged, but more importantly it marks the preludeUniversity of Minnesota (UMN) by immunofluorescence microscopy (IF) of HeLa cells expressing the A3B-eGFP protein. Rabbits showing positive anti-A3B immune responses were selected for a final immunization boost before their spleens were harvested for B-cell isolation and hybridoma production. Hybridoma fusions of 240E-W cells with lymphocytes from the selected rabbits were performed by Epitomics in 40 × 96-well plates. Cell supernatants were screened at UMN by A3B ELISA and the strongest positive hybridoma pools were subcloned by standard limiting dilution to generate monoclonal hybridoma cell lines. Hybridoma 5210-87-13 was expanded to 1 L and then switched to a serum-free medium for one week. This medium was clarified by centrifugation to remove cells and then passed over a Protein A column to bind mAb. The resulting mAb was eluted


[Section 1](#sec1-antibodies-08-00047){ref-type="sec"}), cancer cell lines were selected for additional 5210-87-13 IB validation studies. First, two multiple myeloma cell lines (NCI-H929 and JJN3) and two primary effusion lymphoma cell lines (BC-3 and JSC-1) were tested, due to RNAseq data indicating relatively high *A3B* expression \[[@B62-antibodies-08-00047]\]. These immunoblots showed strong reactivity around 36 kDa, relative to the molecular weight size marker, which is the position where endogenous A3B typically migrates ([Figure 3](#antibodies-08-00047-f003){ref-type="fig"}A). Endogenous A3G migrates slightly slower with, for instance, BC-3 expressing high A3B and low A3G and the other lines expressing similar levels of both proteins. The identity of the 36 kDa A3B band was confirmed in JJN3 cells through two independent CRISPR knockouts ([Figure 3](#antibodies-08-00047-f003){ref-type="fig"}A). It is important to note that both A3B and A3G migrate faster inare still interpretable and, in many instances, definitive, as: (1) A3B was the only one of these enzymes expressed in the system (e.g., transient transfections and many different cancer cell lines and tumor tissues); (2) the three enzymes can be distinguished by kilodalton size and mobility (immunoblots); and (3) the enzymes localize to different subcellular compartments (A3B shows nuclear localization, whereas A3A and A3G are cytoplasmic). The experiments reported here also show that the 5210-87-13 mAb does not cross-react with more distantly related human APOBEC3 family members (i.e., A3C/D/F/H, AID, and APOBEC1).

The 5210-87-13 mAb detailed here has already proven effective in a range of IB experiments. We first used it to study induction of endogenous A3B expression by the PKC/non-canonical NF-κB signal transduction pathway \[[@B27-antibodies-08-00047]\]. We subsequently used


obtained also considering the dependency among tests. The analysis here performed can be also viewed as a kind of multi-point analysis. We further pursue this comparison by looking at the prediction errors, Λ, of the estimated Random Forest model, , based only on the most important SNP and its distance from the HBB gene. We have one important SNP for each data set and this SNP has been selected using the Bagging approach as detailed in the Methods Section. The Bagging approach is enough in this application because the Beta-Thalassemia is a monogenic disease. Prediction errors and distances, for all samples, are reported in Table [1](#T1){ref-type="table"}. We can see that only using sample **D**, the prediction error is the lowest (12%) and the selected SNP is also oneit is also located at 580 *Kb*from the HBB gene farther away from the SNP obtained with .

The final message conveyed by Figure [1](#F1){ref-type="fig"} and Table [1](#T1){ref-type="table"} is that the subsample **D**has lower genetic spurious variability than other subsamples obtained ignoring the genealogy. Finally, using with the genealogical tree we are more likely to trace back the position of the mutation point causing Beta-Thalassemia.

Application to Asthma
---------------------

Over a data set of 208 genotyped subjects underwent clinical and instrumental examination, we dispose of a total of *I*= 57 asthmatic cases and *J*= 151 non asthmatic potential controls. For each subject we measured breath nitric oxide, forced expiratory volume (FEV1) and blood IgE levels. Moreover a standardized epidemiological questionnaire was administered to all participants to the study. The affection status has


the limitations of the simulation study, results reflect what found with empirical evidence: tends to perform better with respect to other methods. It improves when the subjects are selected according to the Hungarian method because of the reduced amount of noise on *P*- *p*non important SNPs. The local false discovery rate and the Q-values perform worse than and the BH method because they are adaptive methods in the sense that they make an estimation of the null distribution of the *p*-values, while the BH method is not adaptive (for more details see \[[@B28]\]). Estimating the null distribution may be problematic because the sample size is quite small and the observed *p*-values from the Fisher test either concentrate on 0 or on 1. This result validates the choice ofof **K**.

The design of such experiment provides a balanced case/control study with 2*I*individuals, where spurious genetic variability is supposed to have been reduced.

Model
-----

In order to obtain the prediction model, *F*(**x**), for a certain omic profile, **x**, we propose a procedure based on Random Forest described in \[[@B29]\]. is a non parametric regression model and its use in genetic association studies grows only in the very recent years. In particular, \[[@B27]\] and \[[@B30]\] use it with expression data, where the considered values of *P*are of order of a few thousands and the observations are assumed independent. On the contrary, most genetic association studies consider only one genetic variant at time and rarely take into account the interactions between them \[[@B12]\]. Other papers, based on multiple testing \[[@B23],[@B31]\], take into


row locates the theoretical mutation points related to the disease, while the upper row locates the observed points. The triangle corresponds to an observed mutation that able us to trace back the theoretical mutation point.](1471-2156-12-63-5){#F5}

Association studies aim at discovering causal variants that are responsible for the disease and these can be located by those SNPs that are nearest to the causal variant. In order to have a good prediction model, we should exclude all SNPs that, although having some predictive power on the outcome *Y*, are far from the causal variant and they are essentially false positives. Statistically speaking, we have to make a screening among all SNPs before proceeding to estimate the final model *F*(**x**). After the screening phase we construct the final model, by selecting the      cleaned.append('' + dirty + ', ' + '')

        address = address.lower()
        address = re.sub('[^A-Za-z0-9#]+', ' ', address).lstrip()

        pattern = r"\d+.* +(\d+ .*(" + "|".join(patterns) + "))"
        address = re.sub(pattern, "\\1", address)

        address = check_st(address)

        if 'one ' in address:
            address = address.replace('one', '1')
        if 'two' in address:
            address =


     number_location = address[location]
                    #print address[location]
                    #if 'th' in address[location + 1] or 'floor' in address[location + 1] or '#' in address[location]:
                    #    continue
            except (ValueError, IndexError):
                continue
                    address = address.replace('havenue', 'h avenue')
        if 'davenue' in address:
            address = address.replace('davenue', 'd avenue')

        #print address

        regex = r'(.*)(' + '|'.join(patterns) + r')(.*)'
        address = re.sub(regex, r'\1\2', address).lstrip() + " nyc"

        print (address)

        if 'americasas st' in address:
            address = address.replace('americasas st', 'americas')

        try: