Conditions: Huntington's Disease
Allocation: RANDOMIZED | Intervention model: SEQUENTIAL
Enrollment: 88
Healthy volunteers: no

Brief summary:
PRECISION-HD2 is a Phase 1b/2a multicenter, randomized, double-blind, placebo-controlled study to evaluate the safety, tolerability, pharmacokinetics (PK), and pharmacodynamics (PD) of single and multiple doses of WVE-120102 in adult patients with early manifest Huntington's disease (HD) who carry a targeted single nucleotide polymorphism (SNP) rs362331 (SNP2).

Detailed description:

Arms:
arm 1: None arm 2: None arm 3: None arm 4: None arm 5: None

Interventions: intervention 1: WVE-120102 intervention 2: Placebo
Intervention descriptions: intervention 1: WVE-120102 is a stereopure antisense oligonucleotide (ASO) intervention 2: 0.9% Sodium Chloride

Protocol document:
Prot_SAP_000.pdf: 
WVE-120102 
 
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Clinical Study Protocol: WVE-HDSNP2-001 
 
17 September 2019 Amendment 2.0 
 
 
Page 1 
Clinical Study Protocol 
A Multicenter, Randomized, Double-blind, Placebo-
controlled, Phase 1b/2a Study of WVE-120102 Administered 
Intrathecally in Patients with Huntington’s Disease  
WVE-HDSNP2-001 
 
Drug Development Phase: 
Phase 1b/2a 
Investigational Product: 
WVE-120102 
Indication: 
Huntington’s disease 
EudraCT Number 
2016-005142-39 
Sponsor: 
Wave Life Sciences UK Limited  
Hays Galleria 
1 Hays Lane 
London, SE1 2RD 
United Kingdom 
Amendment  
2.0 
Date: 
17 September 2019 
Conduct:  In accordance with the ethical principles that originate from the Declaration of 
Helsinki and that are consistent with International Conference on Harmonisation (ICH) 
guidelines on Good Clinical Practice (GCP) and local regulatory requirements as applicable. 
 
 


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PROTOCOL APPROVAL SIGNATURE PAGE 
SPONSOR:  WAVE LIFE SCIENCES LTD. 
I have read and understand the contents of this clinical protocol for Study No. 
WVE-HDSNP2-001 dated 17 September 2019 and agree to meet all obligations of the Sponsor 
as detailed in all applicable regulations and guidelines.  In addition, I will inform the Principal 
Investigator and all other Investigators of all relevant information that becomes available 
during the conduct of this study. 
 
 


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PRINCIPAL INVESTIGATOR’S AGREEMENT 
I have read and understand the contents of this clinical protocol for Study No. 
WVE-HDSNP2-001 dated 17 September 2019 and will adhere to the study requirements as 
presented, including all statements regarding confidentiality.  In addition, I will conduct the 
study in accordance with current International Conference on Harmonization guidelines 
governing Good Clinical Practices, applicable Food and Drug Administration (FDA) regulations, 
and other local regulatory requirements: 
Name of Principal Investigator: 
Title: 
Institution: 
Address: 
Phone:   
Fax:   
 
 
 
Signature 
 
Date 
 
 
 


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Clinical Study Protocol: WVE-HDSNP2-001 
 
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PROTOCOL SYNOPSIS 
Sponsor: 
Wave Life Sciences Ltd. 
Investigational Product: 
WVE-120102 
Phase: 
1b/2a 
EudraCT Number:  
2016-005142-39 
Title of Study:  A Multicenter, Randomized, Double-blind, Placebo-controlled, Phase 1b/2a 
Study of WVE-120102 Administered Intrathecally in Patients with Huntington’s Disease  
Protocol Number:  WVE-HDSNP2-001 
Study Center(s):  Approximately 30 study sites worldwide 
Indication:  Huntington’s disease (HD)  
Objectives: 
Primary objective: 
• Evaluate the safety and tolerability of WVE-120102 in patients with early manifest HD.  
Secondary objectives: 
• Characterize the pharmacokinetics (PK) of WVE-120102 in plasma.  
• Characterize the exposure of WVE-120102 in cerebrospinal fluid (CSF).  
• Assess the pharmacodynamic (PD) effect of WVE-120102 on levels of mutant huntingtin 
protein (mHTT) in CSF. 
• Assess the effect of WVE-120102 on signs and symptoms of HD, as measured by the 
Total Functional Capacity (TFC), administered as part of the Unified Huntington’s 
Disease Rating Scale (UHDRS).  
Exploratory Objectives: 
• Characterize changes in magnetic resonance imaging (MRI) of the brain in patients 
receiving WVE-120102. 
• Assess the effect of WVE-120102 on signs and symptoms of HD, as measured by the 
motor, cognitive, independence, and functional assessments, administered as part of the 
UHDRS. 
• Assess the effect of WVE-120102 on signs and symptoms of HD, as measured by the 
Short Problem Behaviors Assessment (PBA-s). 
Methodology:  This is a Phase 1b/2a multicenter, randomized, double-blind, placebo-
controlled study to evaluate the safety, tolerability, PK, and PD of intrathecal (IT) WVE-
120102 in adult patients with early manifest HD who carry a targeted single nucleotide 
polymorphism (SNP) rs362331 (SNP2).  
This study will evaluate a single dose of WVE-120102 followed by multiple doses in eligible 
patients with early manifest HD.  To participate in the study, patients must have documented 
heterozygosity for SNP2 with the thymine (T) variant on the same allele as the cytosine-
adenine-guanine (CAG) triplet expansion.  Patients who have not previously undergone SNP 
analysis will be required to be prescreened to determine heterozygosity for SNP2.  The 
prescreening assessment will have a separate informed consent form (ICF).   
Patients may participate in the complete study, in which they will receive all 4 doses.  This is 
a global protocol with some regions only participating in the single-dose component of the 


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Sponsor: 
Wave Life Sciences Ltd. 
Investigational Product: 
WVE-120102 
Phase: 
1b/2a 
EudraCT Number:  
2016-005142-39 
study.  In order to allow for evaluation of a full multi-dose cohort (N=12), new patients may 
be enrolled directly in the multiple-dose portion to account for those patients who do not roll 
over from the single-dose portion to the multi-dose portion.  These patients will receive 3 
doses.  Five dose cohorts will be evaluated (2, 4, 8, 16, and 32 mg).   
Single Dose Portion 
In each cohort, 12 patients (9 active and 3 placebo) will receive a single dose of WVE-
120102 or placebo.  Dosing will be initiated in a staggered manner, in which 2 sentinel 
patients (1 placebo and 1 active) will be dosed and observed for 48 hours in the clinic.  If 
neither patient experiences a serious adverse event (SAE) during that period, the remaining 
10 patients (2 placebo and 8 WVE-120102) will be dosed sequentially.  Patients in the 2, 4, 8, 
and 16 mg dose cohorts will be assessed for safety, PK, PD, and clinical effects through the 
8-week washout period after the first dose. Patients in the 32 mg cohort will be assessed for 
safety, PK, PD, and clinical effects through 4 weeks after the first dose.  
Multiple Dose Portion 
Following a safety review of the single-dose data from all the patients in a dose cohort, the 
cohort will proceed to multiple dosing.  Patients will receive 3 doses of WVE-120102 in the 
multiple-dose portion, administered once every 4 weeks for a period of 8 weeks.  Patients in 
the 2, 4, 8, and 16 mg cohorts will be assessed for safety, PK, PD, and clinical effects through 
Week 30 (14 weeks after the last dose). Patients in the 32 mg cohort will be assessed for 
safety, PK, PD, and clinical effects through Week 26 (14 weeks after the last dose).  
Patients who drop out may be replaced.  
Following completion of this study, all patients may be eligible to participate in an open-label 
extension study regardless of whether they participated in only the single dose portion, 
multiple dose portion, or both parts of the study.    
Dose Escalation Committee Review 
The decision regarding escalation to each subsequent dose and whether a single dose can 
proceed to multiple dosing will be made by the Dose Escalation Committee.  The Dose 
Escalation Committee will be blinded to treatment assignment throughout the study.  All 
recommendations of this committee will be reviewed by Safety Monitoring Committee 
(SMC) and endorsed by the chairperson.  
Safety Monitoring Committee Review 
Throughout the study, unblinded aggregate safety data will be reviewed periodically and on 
an ad hoc basis by the SMC.  The SMC will review any SAEs that occur in sentinel patients 
in order to determine if the cohort should continue or if a lower dose should be selected.  In 
addition, if treatment-emergent adverse events (TEAEs) occur that meet the Stopping 
Criteria, the SMC will review the unblinded safety data and determine whether it is safe to 
proceed with the cohort.     
Stopping Criteria 
During the single-dose, dose escalation phase, dosing will be suspended: 


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Investigational Product: 
WVE-120102 
Phase: 
1b/2a 
EudraCT Number:  
2016-005142-39 
1. If a single patient experiences an SAE assessed as related to study drug; 
2. If ≥2 patients experience a TEAE of ≥Grade 3 (using National Cancer Institute Common 
Terminology Criteria for AE [NCI CTCAE] grading) that is assessed as related to study 
drug; or 
3. If ≥4 patients experience a non-serious TEAE of ≥Grade 2 within the Medical Dictionary 
for Regulatory Activities (MedDRA) High Level Group Term (HLGT) Spinal Cord and 
Nerve Root Disorders, assessed as related to study drug. 
Adverse event (AE) terms related to lumbar puncture and administration (e.g., procedural 
pain, traumatic lumbar puncture, or post-lumbar puncture syndrome) will be exempted from 
the assessment of Stopping Criteria.  A full list of AE terms exempt from the Stopping 
Criteria is provided in Table 4 in Section 4.3.4.1. 
If a determination is made to resume the study, information will be submitted to the 
regulatory authorities in accordance with local regulations prior to restarting treatment. 
During the multiple-dose phase, dosing will be stopped for an individual patient if: 
1. Patient experiences a serious or intolerable adverse event (AE), that in the Investigator’s 
opinion requires study drug discontinuation.   
2. Patient experiences the same related TEAE ≥Grade 3 twice (excepting exempt TEAEs 
noted in Table 4). 
If a patient meets the individual stopping criteria in the multiple-dose phase, that patient will 
not receive any more doses of WVE-120102.  The patient will be followed up for safety per 
protocol. 
Number of Patients:  Approximately 80 patients will be enrolled. 
Study Population:  Patients must satisfy all of the inclusion and none of the exclusion 
criteria to be eligible for the study.  If the patient does not have documented heterozygosity at 
SNP2, the patient must undergo Prescreening.   
Inclusion Criteria: 
1. Documented ability to understand the written study ICF(s) and has provided signed 
written informed consent prior to any study procedures. 
2. Ambulatory male or female. 
3. Age ≥25 to ≤65 years old. 
4. Body mass index (BMI) ≤32 kg/m2. 
5. Documented CAG triplet repeats ≥36 in the Huntingtin gene. 
6. Documented heterozygosity for SNP2. 
7. Documented presence of the T variant of SNP2 on the same allele as the pathogenic CAG 
expansion 
8. Clinical diagnostic motor features of HD, defined as UHDRS Diagnostic Confidence 
Score = 4.  


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Sponsor: 
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Investigational Product: 
WVE-120102 
Phase: 
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EudraCT Number:  
2016-005142-39 
9. Stage I or Stage II HD, defined as UHDRS TFC scores ≥7 and ≤13. 
10. In the opinion of the Investigator, the patient is able to tolerate all study procedures, and 
is willing to comply with all other protocol requirements. 
11. Willingness to practice highly effective contraception for the duration of the study if 
patients or their partners are of childbearing potential.  Non-childbearing potential and 
highly effective methods of contraception are defined in the protocol (Section 5.2.1). 
Exclusion Criteria: 
1. Malignancy or received treatment for malignancy, other than treated basal cell or 
squamous cell carcinoma of the skin, within the previous 5 years. 
2. Positive for Hepatitis B virus (HBV) or Hepatitis C virus (HCV). 
3. Known to be positive for human immunodeficiency virus (HIV). 
4. Clinically significant medical finding on the physical examination other than HD that, in 
the judgment of the Investigator, will make the patient unsuitable for participation in 
and/or completion of the study procedures. 
5. Received an investigational drug, including an investigational oligonucleotide, within the 
past 1 year or 5 half-lives of the drug, whichever is longer. 
6. Implantable central nervous system (CNS) device that may interfere with ability to 
administer study drug via lumbar puncture or undergo MRI scan. 
7. Diagnostic and Statistical Manual of Mental Disorders 5th Edition (DSM-5) diagnosis at 
the Screening Visit of active alcohol, cannabinoid, or other substance use disorder (except 
nicotine) within 6 months prior to the Screening Visit. 
8. Positive for opioids (unprescribed), cocaine, amphetamines, methadone, barbiturates, 
methamphetamine, or phencyclidine at the Screening Visit.   
9. Started or changed dose for concomitant medication for the treatment of HD symptoms or 
psychiatric disorders within 30 days prior to the Screening Visit (concomitant 
medications that have been administered on a stable regimen for ≥30 days are permitted). 
10. Pregnant (as determined by a serum pregnancy test) or breast feeding at the Screening 
Visit, or plans to become pregnant during the course of the study. 
11. Clinically significant laboratory abnormality at Screening, including, but not limited to: 
a 
Alanine aminotransferase (ALT) or aspartate aminotransferase (AST) values at 
Screening or Baseline >3 times the upper limit of normal (ULN). 
b Renal insufficiency, defined as either serum creatinine >1.8 mg/dL or creatinine 
clearance <40 mL/min. 
12. Clinically significant abnormality at Screening electrocardiogram (ECG), including but 
not necessarily limited to a confirmed QT interval corrected for heart rate (QTc) 
≥450 msec for males or ≥470 msec for females. 
13. Clinically significant cardiovascular, endocrine, hepatic, renal, pulmonary, 
gastrointestinal, neurologic, malignant, metabolic, psychiatric, or other condition that, in 
the opinion of the Investigator, precludes the patient’s safe participation in the study or 
would interfere with the study assessments.   


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Phase: 
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EudraCT Number:  
2016-005142-39 
14. Bone, spine, bleeding, or other disorder that exposes the patient to risk of injury or 
unsuccessful lumbar puncture. 
15. Inability to undergo brain MRI (with or without sedation).   
16. Deemed to be at significant risk for suicidal behavior based on: 
a 
The opinion of the Investigator; or 
b Answers “yes” to Actual Suicide Attempts or Suicidal Behaviors in the Suicidal 
Behaviors section of the Columbia-Suicide Severity Rating Scale (C-SSRS) with 
reference to a 2-year period prior to the Screening Visit; or 
c 
Answers “yes” on any items in the Suicidal Ideation section of the C-SSRS with 
reference to a 6-month period prior to the Screening Visit; or 
d Answers “yes” on any items in the Suicidal Ideation section of the C-SSRS at the 
Baseline Visit since the last visit (Screening Visit). 
17. Involved directly or indirectly in the conduct and administration of this study as an 
Investigator, sub-investigator, study coordinator, or other study staff member, or the 
patient is a first-degree family member, significant other, or relative residing with one of 
the above persons involved directly or indirectly in the study. 
Test Product, Dose and Mode of Administration:   
The starting dose will be 2 mg.  Planned dose cohorts are 2, 4, 8, 16, and 32 mg.  The dosage 
form will be a lyophilized powder for reconstitution for solution for injection.  The route of 
administration of the reconstituted study drug in solution will be IT injection by direct lumbar 
puncture.  
Reference Therapy, Dose, and Mode of Administration:   
Placebo will be 0.9% Sodium Chloride Injection, sterile, preservative-free solution.  Placebo 
will be visually identical in appearance to the WVE-120102 injection solution and 
administered intrathecally in order to maintain the blind.  
Study Duration:   
Doses for the 2, 4, 8, and 16 mg dose cohorts will be administered on Days 1, 56, 84, and 
112, with an additional 14-week follow-up. Doses for the 32 mg cohort will be administered 
on Days 1, 28, 56, and 84, with an additional 14-week follow-up.  
Criteria for Evaluation: 
Safety 
Adverse events, concomitant medications, physical examinations including detailed 
neurological examination, vital signs, 12-lead ECGs, clinical laboratory evaluations 
(including clinical chemistry, hematology, and urinalysis), CSF evaluations (total protein, 
glucose, and white blood cell counts with differential), MRI of the brain, and C-SSRS. 
Pharmacokinetics 
• Pharmacokinetic parameters of WVE-120102 in plasma at predefined time points.   
• Exposure of WVE-120102 in CSF at predefined time points.  


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Sponsor: 
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Investigational Product: 
WVE-120102 
Phase: 
1b/2a 
EudraCT Number:  
2016-005142-39 
Pharmacodynamics 
• Concentration of mHTT protein in CSF predose (baseline value) and at the last 
measured time point. 
Clinical Effects 
• Change from baseline (baseline value to the last measured time point) and difference 
from placebo in the TFC, administered as part of the UHDRS. 
• Change from baseline (baseline value to the last measured time point) and difference 
from placebo in the motor, cognitive, independence, and functional assessments 
administered as part of the UHDRS. 
• Change from baseline (baseline value to the last measured time point) and difference 
from placebo in the PBA-s. 
• Changes from baseline MRI of the brain (Screening to last measured time point). 
Statistical Methods:  In general, summary statistics (n, mean, standard deviation [SD], 
median, minimum and maximum values for continuous variables, and number [%] of patients 
in each category for categorical variables) will be provided by dose cohort and visit. Patient-
level data will be presented in data listings. 
Analyses will be primarily descriptive; confidence intervals will be presented where 
appropriate.   
The sample size was not calculated on the basis of statistical hypothesis testing. However, 
based on the results of Wild et al.1, for each of the active doses, 9 patients per active dose will 
yield a two-sided 90% confidence interval width for mHTT in CSF of approximately ±110 
fM.  
 
 


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TABLE OF CONTENTS 
PROTOCOL SYNOPSIS ..........................................................................................................4 
TABLE OF CONTENTS .........................................................................................................10 
LIST OF ABBREVIATIONS ..................................................................................................15 
1 INTRODUCTION .............................................................................................................18 
1.1 
Huntington’s Disease .............................................................................................18 
1.2 
Investigational Product WVE-120102 ...................................................................18 
1.3 
Nonclinical Data ....................................................................................................19 
1.4 
Clinical Experience ................................................................................................21 
2 RATIONALE FOR THE STUDY .....................................................................................21 
2.1 
Rationale for the Doses and the Dosing Regimen .................................................21 
3 STUDY OBJECTIVES AND ENDPOINTS .....................................................................23 
3.1 
Study Objectives ....................................................................................................23 
3.2 
Study Endpoints .....................................................................................................23 
4 STUDY DESIGN...............................................................................................................24 
4.1 
Study Design Overview .........................................................................................24 
4.1.1 
Prescreening Phase.....................................................................................26 
4.1.2 
Screening Phase .........................................................................................26 
4.1.3 
Single-Dose Phase .....................................................................................27 
4.1.4 
Multiple-Dose Phase ..................................................................................28 
4.1.5 
Follow-up Phase.........................................................................................28 
4.2 
Schedule of Assessments .......................................................................................29 
4.3 
Safety Reviews, Stopping Criteria, and Communication plan ..............................39 
4.3.1 
Safety Monitoring Committee Reviews.....................................................39 
4.3.2 
Safety Review After Sentinel Patients .......................................................39 
4.3.3 
Dose Escalation and Continuation to Multiple Dosing..............................39 
4.3.4 
Stopping Criteria ........................................................................................40 
4.3.5 
Communication of Emergent Safety Concerns ..........................................42 
5 PATIENT SELECTION AND WITHDRAWAL CRITERIA ..........................................42 
5.1 
Inclusion Criteria ...................................................................................................42 
5.2 
Exclusion Criteria ..................................................................................................43 
5.2.1 
Additional Study Restrictions ....................................................................44 
5.3 
Withdrawal Criteria ...............................................................................................45 
5.3.1 
Reasons for Withdrawal/Discontinuation ..................................................45 
5.3.2 
Handling of Withdrawals ...........................................................................45 
5.3.3 
Replacements .............................................................................................46 


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6 STUDY TREATMENTS ...................................................................................................46 
6.1 
Method of Assigning Patients to Study Drug ........................................................46 
6.2 
Dose and Study Drug Administration ....................................................................46 
6.2.1 
Identity of Study Drug ...............................................................................46 
6.2.2 
Administration of Study Drug and Collection of CSF...............................47 
6.3 
Treatment Compliance ...........................................................................................47 
6.4 
Management of Clinical Supplies ..........................................................................48 
6.4.1 
Study Drug Packaging and Storage ...........................................................48 
6.4.2 
Study Drug Accountability ........................................................................48 
6.5 
Other Supplies ........................................................................................................48 
7 BLINDING ........................................................................................................................48 
7.1 
Blinding and Randomization .................................................................................48 
7.2 
Breaking the Blind .................................................................................................49 
8 METHODS OF ASSESSMENT AND ENDPOINTS .......................................................49 
8.1 
Prescreening Assessments .....................................................................................49 
8.2 
Safety Assessments ................................................................................................50 
8.2.1 
Medical History and Demographics ..........................................................50 
8.2.2 
Prior and Concomitant Medications ..........................................................51 
8.2.3 
Physical Examination.................................................................................51 
8.2.4 
Vital Signs ..................................................................................................51 
8.2.5 
12-Lead ECG .............................................................................................52 
8.2.6 
Clinical Laboratory Evaluations ................................................................52 
8.2.7 
Cerebrospinal Fluid Safety Lab .................................................................52 
8.2.8 
Suicidality Assessment (Columbia-Suicide Severity Rating Scale) ..........53 
8.2.9 
Pregnancy Testing ......................................................................................53 
8.2.10 
Drug Screening ..........................................................................................53 
8.3 
Pharmacokinetic Assessments ...............................................................................53 
8.4 
Pharmacodynamic Assessments ............................................................................54 
8.5 
Immunogenicity Assessments ................................................................................54 
8.6 
Magnetic Resonance Imaging ................................................................................54 
8.7 
Assessment of Clinical Effects ..............................................................................55 
8.7.1 
Unified Huntington’s Disease Rating Scale ..............................................55 
8.7.2 
Short Problem Behaviors Assessment .......................................................55 
9 ADVERSE EVENTS .........................................................................................................56 
9.1 
Eliciting and Documenting Adverse Events ..........................................................56 
9.2 
Definitions of Adverse Event Severity and Relationship to Study Drug...............57 


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9.2.1 
Severity ......................................................................................................57 
9.2.2 
Relationship to Study Drug ........................................................................57 
9.3 
Serious Adverse Events .........................................................................................58 
9.3.1 
Serious Adverse Event Criteria ..................................................................58 
9.3.2 
Serious Adverse Event Follow-up .............................................................58 
9.3.3 
Serious Adverse Event Reporting ..............................................................59 
9.4 
Overdose ................................................................................................................60 
9.5 
Pregnancies ............................................................................................................60 
10 STATISTICAL METHODS ..............................................................................................60 
10.1 
General Considerations ..........................................................................................60 
10.2 
Sample Size Determination....................................................................................60 
10.3 
Study Patients.........................................................................................................61 
10.3.1 
Analysis Populations ..................................................................................61 
10.3.2 
Patient Disposition .....................................................................................61 
10.3.3 
Demographics and Other Baseline Characteristics ....................................61 
10.3.4 
Protocol Deviations ....................................................................................61 
10.3.5 
Prior and Concomitant Medications ..........................................................62 
10.3.6 
Study Drug Exposure and Compliance ......................................................62 
10.4 
Pharmacokinetic Analyses .....................................................................................62 
10.5 
Pharmacodynamic Analyses ..................................................................................63 
10.6 
Immunogencity assessment ...................................................................................63 
10.7 
Magnetic Resonance Imaging ................................................................................63 
10.8 
Clinical Effects Analyses .......................................................................................63 
10.8.1 
Unified Huntington’s Disease Rating Scale ..............................................63 
10.8.2 
Short Problem Behaviors Assessment .......................................................64 
10.9 
Safety Analyses ......................................................................................................64 
10.9.1 
Adverse Events ..........................................................................................64 
10.9.2 
Clinical Laboratory Evaluations ................................................................64 
10.9.3 
Vital Signs ..................................................................................................64 
10.9.4 
Electrocardiogram ......................................................................................65 
10.9.5 
Magnetic Resonance Imaging (Safety) ......................................................65 
10.9.6 
Cerebrospinal Fluid Safety Lab .................................................................65 
10.9.7 
Columbia-Suicide Severity Rating Scale ...................................................65 
10.10 Interim Analyses ....................................................................................................65 
10.11 Data Quality Assurance .........................................................................................65 
10.11.1 
Electronic Case Report Forms and Data Management ..............................65 


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11 REGULATORY, ETHICAL, AND LEGAL OBLIGATIONS .........................................66 
11.1 
Declaration of Helsinki ..........................................................................................66 
11.2 
Good Clinical Practice ...........................................................................................66 
11.3 
Institutional Review Boards/Ethics Committees ...................................................66 
11.4 
Informed Consent Forms .......................................................................................66 
12 INVESTIGATOR’S OBLIGATIONS ...............................................................................67 
12.1 
Confidentiality .......................................................................................................67 
12.2 
Investigator Documentation ...................................................................................67 
12.3 
Study Conduct ........................................................................................................68 
12.4 
Adherence to Protocol............................................................................................68 
12.5 
Adverse Events and Study Report Requirements ..................................................68 
12.6 
Investigator’s Final Report ....................................................................................68 
12.7 
Records Retention ..................................................................................................68 
12.8 
Publications ............................................................................................................68 
13 STUDY COMMITTEES ...................................................................................................69 
13.1 
Safety Monitoring Committee ...............................................................................69 
13.2 
Dose Escalation Committee ...................................................................................69 
13.3 
Clinical Advisory Committee ................................................................................69 
13.4 
Publications Committee .........................................................................................69 
14 STUDY MANAGEMENT ................................................................................................69 
14.1 
Monitoring .............................................................................................................69 
14.1.1 
Monitoring of the Study .............................................................................69 
14.1.2 
Inspection of Records ................................................................................70 
14.2 
Management of Protocol Amendments and Deviations ........................................71 
14.2.1 
Modification of the Protocol ......................................................................71 
14.2.2 
Protocol Deviations ....................................................................................71 
14.3 
Study Termination .................................................................................................71 
14.4 
Final Report ...........................................................................................................71 
15 REFERENCES ..................................................................................................................73 


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LIST OF IN-TEXT TABLES 
Table 1 
Schedule of Assessments for Patients Participating in Both the Single and 
Multiple Dose Portions of the Study (2, 4, 8, and 16 mg Cohorts) ..............30 
Table 2 
Schedule of Assessments for Patients Participating in Both the Single and 
Multiple Dose Portions of the Study (32 mg Cohort) ...................................33 
Table 3 
Schedule of Assessments for Treatment-Naïve Patients in the Multiple-Dose 
Portion of the Study ......................................................................................36 
Table 4 
Adverse Event Preferred Terms Exempted From Stopping Criteria ............41 
Table 5 
Clinical Laboratory Parameters ....................................................................52 
Table 6 
Definitions of AE Severity Using NCI CTCAE Criteria ..............................57 
Table 7 
Guidelines for Determining the Relationship (if any) Between Adverse Event 
and the Study Drug .......................................................................................58 
Table 8 
Pharmacokinetic Parameters of WVE-120102 after Single-Dose Administration
 ......................................................................................................................63 
Table 9 
Pharmacokinetic Parameters of WVE-120102 after Multiple Dose 
Administration ..............................................................................................63 
LIST OF IN-TEXT FIGURES 
Figure 1 
Schematic of Study Design ...........................................................................26 
LIST OF APPENDICES 
Appendix 1 
Unified Huntington’s Disease Rating Scale .................................................75 
Appendix 2 
Columbia-Suicide Severity Rating Scale .....................................................122 
Appendix 3 
Short Problem Behaviors Assessment (PBA-s) ..........................................129 


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LIST OF ABBREVIATIONS 
Abbreviation 
Definition 
3T 
3 Tesla 
AE 
adverse event 
ALT 
alanine aminotransferase 
aPTT 
activated partial thromboplastin time 
ASO 
antisense oligonucleotide 
AST 
aspartate aminotransferase 
ATC 
Anatomical Therapeutic Chemical 
AUC0-t 
area under the plasma concentration-time curve from time zero to the last quantifiable 
concentration 
AUC0-τ  
area under the plasma concentration-time curve over the dosing interval  
BMI 
body mass index 
CAG 
cytosine-adenine-guanine 
CFR 
Code of Federal Regulations 
CLIA 
Clinical Laboratory Improvement Amendments 
Cmax 
maximum observed concentration 
CNS 
central nervous system 
CRO 
contract research organization 
CSR 
clinical study report 
CSF 
cerebrospinal fluid 
C-SSRS 
Columbia-Suicide Severity Rating Scale 
DNA 
deoxyribonucleic acid 
DSM-5 
Diagnostic and Statistical Manual of Mental Disorders 5th Edition 
EC 
Ethics Committee 
ECG 
electrocardiogram 
eCRF 
electronic case report form 
EDC 
electronic data capture 
EHDN 
European Huntington’s Disease Network 
EOS 
end of study 
ET 
early termination 
FDA 
Food and Drug Administration 
GCP 
Good Clinical Practice 
GLP  
Good Laboratory Practice 
HD 
Huntington’s disease 
HBV 
Hepatitis B virus 
HCV 
Hepatitis C virus 
HED 
human equivalent dose 
HLGT 
High Level Group Term 
HIV 
human immunodeficiency virus 


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Abbreviation 
Definition 
HTT, HTT 
Huntingtin gene or huntingtin protein 
IB 
Investigator’s Brochure 
ICF 
informed consent form 
ICH 
International Conference on Harmonisation 
INN 
International Nonproprietary Name 
IRB 
Institutional Review Board 
IT 
intrathecal 
IUD 
intrauterine device  
IUS 
intrauterine hormone-releasing system 
IV 
intravenous 
IXRS 
interactive voice/web response system 
LC-MS/MS 
liquid chromatography-tandem mass spectrometry 
LLOQ  
lower limit of quantification 
MedDRA 
Medical Dictionary for Regulatory Activities 
mHTT, mHTT 
mutant huntingtin gene or protein 
MRI 
magnetic resonance imaging 
mRNA 
messenger ribonucleic acid 
NCI CTCAE 
National Cancer Institute Common Terminology Criteria for Adverse Events 
NOAEL 
no observed adverse effect level 
NOEL 
no-observed-effect level 
PBA-HD 
Problem Behaviors Assessment for HD 
PBA-s 
Short Problem Behaviors Assessment 
PCR 
polymerase chain reaction 
PD 
pharmacodynamics 
PK 
pharmacokinetic 
PS 
phosphorothioate 
PT 
preferred term 
PVG 
pharmacovigilance 
QTc 
QT interval corrected for heart rate 
RNA 
ribonucleic acid 
SAE 
serious adverse event 
SAP 
Statistical Analysis Plan 
SD 
standard deviation 
SDMT 
Symbol Digit Modalities Test 
SMC 
Safety Monitoring Committee 
SNP 
single nucleotide polymorphism 
SNP2 
single nucleotide polymorphism rs362331 
SOC 
system organ class 
SOP 
Standard Operating Procedure 


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Abbreviation 
Definition 
SUSAR 
suspected unexpected serious adverse reaction 
T 
thymine 
TEAE 
treatment-emergent adverse event 
TFC 
Total Functional Capacity 
tmax 
time of occurrence of Cmax 
tx 
treatment 
U 
uracil 
UHDRS 
Unified Huntington’s Disease Rating Scale 
ULN 
upper limit of normal 
USP 
United States Pharmacopoeia 
WHO 
World Health Organization 
wtHTT 
wild-type huntingtin 
λz  
terminal elimination rate constant  
 


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1 
INTRODUCTION 
WVE-120102 is intended as a disease-modifying agent for the treatment of patients with 
Huntington’s disease (HD).  It is a stereopure antisense oligonucleotide (ASO) intended to 
selectively target the mutant form of the huntingtin (mHTT) gene transcript.     
1.1 
HUNTINGTON’S DISEASE 
Huntington’s disease is a rare, progressive neurological disease that results in motor, cognitive, 
and psychiatric disability and is invariably fatal2.  Because it is a genetic, hereditary disease, it 
can affect multiple family members across generations3.  Although cognitive and psychiatric 
symptoms may develop first, the clinical diagnosis of HD is usually based on the presence of 
chorea, one of the most visually prominent symptoms of this disease.  Chorea is an abnormal 
involuntary movement disorder, which occurs in 90% of patients and is moderate to severe in 
approximately 70% of these patients.  These physical symptoms can appear at any age, but 
typically appear between the ages of 30 and 502.  A physical examination, sometimes combined 
with a neurological examination, can determine whether the onset of the disease has begun.  Life 
expectancy after symptom onset is reduced to around 15 to 20 years2,3.  Prevalence in Europe, 
North America, and Australia is approximately 6 per 100,0004.  In the United States alone, 
approximately 30,000 people have diagnosed HD5,6 and another 200,000 or more Americans 
carry the gene and are at risk of developing the disease6.  Currently, no treatments exist that can 
cure, slow, or reverse the course of HD.  Some of the symptoms of HD can be managed with 
medication and therapies such as antipsychotics and drugs affecting the dopamine pathways, 
which modulate the movement disorder3. 
Huntington’s disease is caused by known mutations on a single gene, characterized by an 
expansion of a cytosine-adenine-guanine (CAG) triplet repeat in the Huntingtin (HTT) gene7.  
Wild-type HTT (wtHTT) protein is critical for neuronal development8.  Although the purpose of 
wtHTT in adults is not completely understood, some studies have shown that it may play an 
important role in neuronal functions8-11.  However, expansion in the CAG triplet repeat in the 
HTT gene results in production of the mHTT protein.  Accumulation of this protein leads to 
progressive loss of neurons in the brain3.  In nonclinical studies, lowering the level of mHTT 
protein as measured in the cerebrospinal fluid (CSF) has been demonstrated to be therapeutic12,13.  
Therefore, a drug that can silence the mHTT gene transcript, while leaving the wild-type allele 
intact, may be able to slow down, stop, or even reverse the course of HD14. 
1.2 
INVESTIGATIONAL PRODUCT WVE-120102 
WVE-120102 is an oligonucleotide, a type of nucleic acid that includes innovative drugs that are 
assembled from chemically modified, short-length ribonucleic acid (RNA) or deoxyribonucleic 
acid (DNA) strands.  The potential therapeutic uses of oligonucleotides include modulating the 
function of target RNAs to affect the production of disease-associated proteins. The mechanism 
of action used by many oligonucleotides, including ASOs, is to promote degradation of the target 
RNA.  Phosphorothioate (PS) modification was one of the earliest and remains one of the most 
common backbone modifications used in oligonucleotide synthesis.  These modifications 
improve the stability, biodistribution, and cellular uptake of oligonucleotides.  The use of PS 
modification in oligonucleotide synthesis creates a chiral center at the phosphorus at each PS, 
each of which has either an “Sp” or “Rp” configuration at random.  A conventional, fully PS-


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modified oligonucleotide (20 nucleotides in length, 19 PS modifications) is a mixture of over 
500,000 stereoisomers (219), each having the same nucleotide sequence but differing in the 
stereochemistry along its backbone, resulting in heterogenous and uncontrolled pharmacological 
properties. 
The Sponsor has developed a proprietary technology that enables the synthesis of PS-modified 
nucleic acid therapeutics in which stereochemistry at each PS position is precisely controlled.  
This degree of control enables rational design and synthesis of optimized, or stereopure, 
oligonucleotides with improved pharmacological and toxicological properties. 
WVE-120102 is a stereopure ASO being developed to selectively target mHTT, leaving wtHTT 
relatively unaffected.  WVE-120102 specifically targets the mHTT messenger ribonucleic acid 
(mRNA) transcript, at the uracil (U) variant of single nucleotide polymorphism (SNP) rs362331 
(SNP2).  A SNP is a single variation in the DNA that can be associated with a mutated gene.  
One of the most frequent SNPs in the mHTT gene is SNP2, which has been shown to be present 
in approximately 45% of patients with HD15.   
1.3 
NONCLINICAL DATA 
The nonclinical program evaluating WVE-120102 to date encompasses in vitro studies of 
primary and secondary pharmacology, preliminary in vitro assessments of drug metabolism, 
standard Good Laboratory Practice (GLP) in vitro and in vivo genetic toxicology, and in vivo 
safety pharmacology and toxicity studies.  
In vitro pharmacology studies confirm that WVE-120102 demonstrates selective knockdown of 
the mHTT mRNA and protein through cleavage of the mutant allele versus the wild-type allele 
with the targeted SNP (SNP2).  This selective knockdown results in greater reduction of the 
mHTT protein over wtHTT protein produced by this RNA and thus, limits the unintended, 
potentially deleterious effects of decreasing wtHTT 8-11.  Because an appropriate animal model 
recapitulating the disease phenotype and the SNP (ie, SNP2) specifically targeted by 
WVE-120102 was not available to the Sponsor before initiating human studies, no in vivo data 
are available regarding the dose required to reduce the level of mHTT or the potential duration of 
knockdown.  The optimal dose and dosing frequency for efficacy in humans will be explored 
during clinical development. 
In vitro, WVE-120102 is highly protein-bound in rat, monkey, and human plasma (>99%). 
Combined results from two bioanalytical assays (bioanalysis and localization) indicate that upon 
IT administration in monkeys, WVE-120102 distributes to perinuclear and nuclear sites in deep 
gray matter structures which are affected by HD pathology. Following single or multiple IT 
administration of WVE-120102 in rats and monkeys, dose-dependent increases were observed in 
plasma, CNS, and systemic tissues (kidney, liver, and spleen). Systemic accumulation of WVE-
120102 after repeated dosing was minimal. In the 13-week and 26-week studies in rats and 
monkeys, CNS tissues showed greater exposure in the spinal cord than in the brain, with highest 
concentrations in the lumbar spinal cord. Among the systemic tissues analyzed, WVE-120102 
concentrations were higher in the kidneys than in liver and spleen. No in vitro CYP inhibition or 
induction was observed at clinically relevant concentrations of WVE-120102. 


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In single and repeat dose IT studies in rats and monkeys, transient, dose-dependent limb 
dysfunction was observed. In rats, findings were observed at dose levels of 0.1 mg (human 
equivalent dose [HED] 56 mg) in a single-dose study and 0.05 mg (HED 28 mg) and above in 
repeat-dose studies. These findings resolved within 72 hours. In monkeys, findings were 
observed at doses up to 6 mg (HED 67.2 mg).  Over the course of 9 months in a chronic study in 
monkeys, affected animals experienced only a single instance of limb dysfunction at sporadic 
time points. In monkeys, only the lower hind limbs were affected and findings were completely 
resolved within 48 hours post-dose. There were no histologic correlates for the clinical finding of 
transient impaired limb function. 
Microscopic CNS findings were observed in both rats and monkeys after IT administration of 
WVE-120102. In rats, after 3 months of repeat IT dosing, cellular infiltrates, minimal to mild 
non-adverse spinal nerve root degeneration, and minute foci of gliosis were observed. The 
findings appeared to be dose- and/or dose-concentration dependent, with minimal effects 
observed at 0.05 mg (2 mg/mL concentration). In monkeys, after 3 to 9 months of repeat IT 
dosing, minimal to mild (3-month study) or minimal to marked (9-month study) mononuclear 
cell infiltrates and minimal to mild microgliosis predominantly in the meninges/epineurium were 
observed at all doses. In the 9-month chronic toxicity study in monkeys, variable concomitant 
fibrosis was also noted at 2 and 4 mg/animal (HED 22.4 mg and 44.8 mg, respectively). These 
findings were considered manifestations of the general response of immune stimulation in 
animals by a foreign substance5 and were considered non-adverse. 
Non-adverse axonal/myelin degeneration (characterized primarily by hypercellularity, Schwann 
cell proliferation, and increased macrophages) was also observed with repeat WVE-120102 
doses in monkeys over 9 months at ≥1 mg/animal (HED 11.2 mg) in females and ≥2 mg/animals 
(HED 22.4 mg) in males. The findings were observed in the nerve roots at all levels of the spinal 
cord, with the highest incidence localized to the nerve roots at the injection site or lumbar nerve 
roots near the IT injection site. These changes were noted in nerve roots in the intrathecal space 
and/or up to the level of the dorsal root ganglion but typically did not extend into adjacent spinal 
nerves or the spinal cord itself. After a recovery period, an active ongoing degenerative process 
was not observed and the observed Schwann cell numbers indicated ongoing recovery.  
WVE-120102 demonstrated minimal potential for pro-inflammatory effects and no potential for 
activated partial thromboplastin time (aPTT) prolongation, hepatotoxicity, or nephrotoxicity21,22, 
which are the recognized systemic toxicities of phosphorothioate oligonucleotides.  In addition, 
WVE-120102 was not associated with any other toxicities, including mutagenicity or effects on 
cardiovascular or respiratory function, and exhibited minimal potential for off-target effects via 
bioinformatic analysis of the human genome.    
A non-dose-related increase in micronucleated polychromatic erythrocytes was observed 
following administration of two SC doses of WVE-120102 in the rat; however, these findings 
were not considered biologically-meaningful, as they were within the historical range for the test 
facility. Kinetochore staining did not suggest that WVE-120102 induces aneugenic or 
clastogenic effects. Moreover, negative in vitro genotoxicity results, the lack of genotoxicity 
within the oligonucleotide class, and lack of observations in the rat and monkey toxicology 
studies to date indicate that WVE-120102 does not pose a genotoxic risk to humans. 


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1.4 
CLINICAL EXPERIENCE 
This is the first clinical study conducted with WVE-120102. For the most up to date clinical 
experience information refer to the Investigator’s Brochure (IB).  
2 
RATIONALE FOR THE STUDY 
Huntington’s disease is an invariably fatal disorder with no known cure.  The accumulation of 
mHTT produced in patients with an abnormal Huntingtin allele leads to a progressive loss of 
neurons in the brain.  Therefore, several therapeutic approaches for HD have focused on reduced 
production or removal of mHTT.  However, at the present time, there are no clinical research 
studies in HD evaluating investigational agents that specifically target mHTT.  Instead, some 
studies target both mutant and wtHTT, with the intent of reducing both forms of the protein to 
levels that will hopefully improve symptoms without leading to the deleterious effects that may 
result from a reduction in wtHTT8-11.  Alternate splicing has been proposed as a mechanism for 
the generation of mHTT; however, ASOs should suppress this as they act on pre-mRNA2.   
WVE-120102 is intended to selectively target mHTT, leaving wtHTT relatively unaffected, by 
targeting the SNP2 variant associated with the pathogenic CAG expansion (≥36 repeats).  This 
should result in selective reduction of the level of mHTT protein.    
2.1 
RATIONALE FOR THE DOSES AND THE DOSING REGIMEN 
The target for WVE-120102 is the mutant HTT (mHTT) mRNA, acting at the U variant of 
SNP2. Since no appropriate animal models that recapitulated the mutation observed in this 
subpopulation of HD patients were available to the Sponsor before initiation of the human 
studies, the proposed therapeutic dose range of 2 to 32 mg was based on the totality of the in 
vitro pharmacology data, which defined the concentration range needed to achieve mHTT 
depletion; the in vivo toxicology studies, which defined the tolerable dose range; and the clinical 
safety data available. The clinical starting dose was selected in consideration of the data from 
both single- and repeat-dose toxicology studies. Escalation to subsequent doses (approximately 
4, 8, 16, and 32 mg) will be based on safety assessments from prior cohorts.  
In vitro pharmacology studies demonstrated selective knockdown of mHTT mRNA and protein 
through preferential cleavage of the mutant allele compared with the wild-type allele. In a study 
conducted in patient fibroblasts that were heterozygous (U/C) or homozygous (U/U) for SNP2, 
treatment with 2.5 µM to 10 µM (17 to 66 μg/mL) WVE-120102 resulted in concentration-
dependent mRNA knockdown and protein reduction for mHTT but no meaningful mRNA 
change or protein reduction for wtHTT in the heterozygous fibroblast. In the homozygous 
fibroblasts, similar mRNA knockdown and protein reduction were observed between total HTT 
and mHTT, confirming target-selectivity. The range of theoretical peak CSF concentrations in 
patients over the proposed dose range of 2 to 32 mg corresponds to 14 to 230 µg/mL. Based on 
the concentrations found to have activity in fibroblasts, the CSF concentrations that correspond 
to the proposed clinical dose range are expected to adequately yield meaningful knockdown of 
mHTT mRNA and protein.  
In the single- and repeat-dose toxicity studies of WVE-120102 in rats and monkeys, dose-related 
limb dysfunction were observed. Additional findings related to limb dysfunction were also 


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observed in the rat, and were likely related to high local concentration of test article within a 
small CNS space as compared to the monkey. Furthermore, the technical complexities of IT 
administration to small animal models may contribute to the findings in the rat.  In monkeys, no 
neurological findings were observed at the lowest dose evaluated in monkeys (2 mg) in either the 
single- or repeat-dose studies.  Application of a 10-fold safety margin to the HED, based on CSF 
volume at the 2-mg dose in monkeys, was used to set the clinical starting dose of 2 mg.  The 
clinical starting dose of 2 mg was also 1/13 the HED of the 0.05-mg dose in rats.  Findings at this 
dose in rats were limited to transient, minimal clinical observations in 3 animals that did not 
recur with continued dosing.  In addition, the dose concentration of 0.2 mg/mL that was 
administered to humans is 1/10 the dose concentration administered to rats at the 0.05-mg dose 
(HED 28 mg) and monkeys at the 2-mg dose (HED 22.4 mg) in the repeat-dose toxicity studies.  
The proposed high dose of 32 mg is expected to produce CSF exposures that are within the range 
of those tested and found to be tolerable in animals. In the monkey, estimated peak CSF 
concentration at the 6 mg no observed adverse effect level (NOAEL) dose (HED 67.2 mg) was 
approximately 0.48 mg/mL (based on an estimated CSF volume of 12.5 mL). In the rat, the 
NOAEL dose of 0.25 mg (HED 140 mg) produced an estimated peak CSF concentration of 1 
mg/mL (based on an estimated CSF volume of 0.25 mL). The 32 mg dose is expected to produce 
a peak CSF concentration of 0.23 mg/mL, which is approximately 2-fold and 4-fold lower than 
those achieved in the monkey and rat, respectively, at the NOAEL-dose levels. To date, data 
from this study indicate that the drug has been well tolerated in patients at all dose levels 
evaluated.  
The study design is based on the need to assess the safety and tolerability of WVE-120102 by 
gradual exposure of a small number of patients with early manifest HD to low single doses of the 
study drug.  If a single dose in a cohort is shown to be safe and well tolerated, that dose level will 
proceed to multiple dosing, and a higher dose will be administered to a subsequent cohort.  
The use of a placebo in the control group is appropriate because there are currently no disease-
modifying treatments for HD. The placebo group will provide a robust reference for safety and 
tolerability of the study drug.  In particular, the placebo will help determine whether adverse 
events noted are related to WVE-120102 itself or to the study procedures (lumbar puncture and 
IT injection). 
After the first dose of WVE-120102 on Day 1, the 32 mg cohort will have a 4-week follow-up 
period instead of an 8-week washout period. The amount of time between the first dose and the 
second dose was reduced because current clinical data suggest that most TEAEs occur within the 
first week after the first dose.  
Follow-up will continue for 14 weeks after the last dose, i.e., to Week 30 for patients in the 2, 4, 
8, and 16 mg cohorts and to Week 26 for patients in the 32 mg cohort.  The 8-week washout 
period was selected based on the distribution of WVE-120102 in the repeat dose (13-week) 
monkey study. At the end of the 8-week recovery period in the repeat-dose study in monkeys, 
mean WVE-120102 levels decreased to ≤2 µg/g in the brain tissue and spinal cord and 
<10 ng/mL in the CSF in the 4- and 6-mg groups.  The concentration of WVE-120102 at 
14 weeks postdose would be anticipated to be less than the lower limit of quantification (LLOQ) 


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in all matrices (brain tissue [LLOQ 0.162 µg/g], spinal cord [LLOQ 0.130 µg/g], and CSF 
[LLOQ 5 ng/mL]).   
Patients have the option to leave the study and be treated per standard care at any time. 
3 
STUDY OBJECTIVES AND ENDPOINTS 
3.1 
STUDY OBJECTIVES 
Primary Objective 
The primary objective of this study is to evaluate the safety and tolerability of WVE-120102 in 
patients with early manifest HD. 
Secondary objectives 
Secondary objectives of this study include: 
• Characterize the pharmacokinetics (PK) of WVE-120102 in plasma.  
• Characterize the exposure of WVE-120102 in CSF.  
• Assess the pharmacodynamic (PD) effect of WVE-120102 on levels of mHTT in CSF.  
• Assess the effect of WVE-120102 on signs and symptoms of HD, as measured by the Total 
Functional Capacity (TFC), administered as part of the Unified Huntington’s Disease Rating 
Scale (UHDRS).  
Exploratory Objectives: 
Exploratory objectives of this study include: 
• Assess the effect of WVE-120102 on signs and symptoms of HD, as measured by the motor, 
cognitive, independence, and functional assessments, administered as part of the UHDRS. 
• Assess the effect of WVE-120102 on signs and symptoms of HD, as measured by the Short 
Problem Behaviors Assessment (PBA-s). 
• Characterize changes in magnetic resonance imaging (MRI) of the brain in patients receiving 
WVE-120102. 
3.2 
STUDY ENDPOINTS 
Primary Endpoint 
The primary endpoint is the safety and tolerability of WVE-120102, as compared with placebo, 
as assessed by the number of patients with adverse events (AEs), severity of AEs, number of 
patients with serious AEs (SAEs), and the number of patients who withdraw due to AEs.  


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Secondary Endpoint(s) 
Secondary endpoints include: 
Pharmacokinetics 
• Pharmacokinetic parameters of WVE-120102 in plasma at predefined time points.   
• Exposure of WVE-120102 in CSF at predefined time points.  
Pharmacodynamics 
• Concentration of mHTT protein in CSF predose (baseline value) and at the last measured 
time point. 
Clinical Effects 
• Change from baseline (baseline value to the last measured time point) and difference from 
placebo in the TFC, administered as part of the UHDRS. 
Exploratory Endpoints 
• Change from baseline (baseline value to the last measured time point) and difference from 
placebo in the motor, cognitive, independence, and functional assessments administered as 
part of the UHDRS. 
• Change from baseline (baseline value to the last measured time point) and difference from 
placebo in the PBA-s. 
• Changes from baseline MRI of the brain (Screening to last measured time point). 
4 
STUDY DESIGN 
4.1 
STUDY DESIGN OVERVIEW 
This is a Phase 1b/2a multicenter, randomized, double-blind, placebo-controlled study to 
evaluate the safety, tolerability, PK, and PD IT WVE-120102 in adult patients with early 
manifest HD who carry a targeted single nucleotide polymorphism, SNP2.  
This study will evaluate a single dose of WVE-120102 followed by multiple doses in eligible 
patients with early manifest HD.  To participate in the study, patients must have documented 
heterozygosity for SNP2 with the thymine (T) variant on the same allele as the CAG triplet 
expansion.  Patients who have not previously undergone SNP analysis will be required to be 
prescreened to determine heterozygosity for SNP2.  The prescreening assessment will have a 
separate informed consent form (ICF).   
Patients may participate in the complete study, in which they will receive all 4 doses.  This is a 
global protocol with some regions only participating in the single-dose component of the study.  
In order to allow for evaluation of a full multi-dose cohort (N=12), new patients may be enrolled 
directly in the multiple-dose portion to account for those patients who do not roll over from the 
single-dose portion to the multi-dose portion.  These patients will receive 3 doses.  Five dose 
cohorts will be evaluated (2, 4, 8, 16, and 32 mg).   


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Single Dose Portion 
In each cohort, 12 patients (9 active and 3 placebo) will receive a single dose of WVE-120102 or 
placebo.  Dosing will be initiated in a staggered manner, in which 2 sentinel patients (1 placebo 
and 1 active) will be dosed and observed for 48 hours in the clinic.  If neither patient experiences 
an SAE during that period, the remaining 10 patients (2 placebo and 8 WVE-120102) will be 
dosed sequentially and observed in the hospital for 24 hours.  Patients in the 2, 4, 8, and 16 mg 
dose cohorts will be assessed for safety, PK, PD, and clinical effects through the 8-week washout 
period after the first dose. Patients in the 32 mg cohort will be assessed for safety, PK, PD, and 
clinical effects through 4 weeks after the first dose.  
Multiple Dose Portion 
Following a safety review of the single-dose data from all the patients in a dose cohort by the 
Dose Escalation Committee (Section 4.3.3), the cohort will proceed to multiple dosing.  Patients 
will receive 3 doses of WVE-120102 in the multiple dose portion, administered once every 
4 weeks (for the  2, 4, 8, and 16 mg cohorts: Day 56, Day 84, and Day 112; for the 32 mg cohort: 
Day 28, Day 56, and Day 84).  Patients in the 2, 4, 8, and 16 mg cohorts will be assessed for 
safety, PK, PD, and clinical effects until Week 30 (14 weeks after the last dose). Patients in the 
32 mg cohort will be assessed for safety, PK, PD, and clinical effects through Week 26 
(14 weeks after the last dose).  
The Dose Escalation Committee will also confirm the dose for the next single-dose cohort 
(Section 4.3.3).  Recommendations will be reviewed by the unblinded, independent Safety 
Monitoring Committee (SMC) and endorsed by the chairperson.   
Patients who drop out may be replaced.  
Following completion of this study, all patients may be eligible to participate in an open-label 
extension study regardless of whether they participated in only the single dose portion, multiple 
dose portion, or both parts of the study.        
A schematic of the overall study design and timing is provided in Figure 1. 


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Figure 1 
Schematic of Study Design  
 
Abbreviations: DEC = Dose Escalation Committee; hr = hour; SAD = single ascending dose; SD = single 
dose. 
4.1.1 
Prescreening Phase  
If documentation of heterozygosity and the presence of SNP2 is not available, patients must 
undergo Prescreening. The prescreening period is intended to identify patients who carry the 
targeted SNP.   
Patients will sign a prescreening ICF specific to the assessments performed to determine whether 
they meet the eligibility criteria for heterozygosity of the targeted SNP, CAG triplet repeats 
(≥36), and presence of the T variant of SNP2 on the same allele as the pathogenic CAG 
expansion (Section 8.1).  This testing process is expected to take at least 6 weeks.  Prescreening 
can happen any time before Screening.  If patients meet these criteria, they will continue to the 
Screening Phase (Visit 2). 
4.1.2 
Screening Phase 
The screening period is intended to allow determination of patient eligibility for the study. It will 
begin when the study informed consent is signed. The ability of the patient to understand the 
consent form will be documented in the study ICF.   


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The Screening period will last up to 4 weeks.  The required screening evaluations are outlined in 
the Schedule of Assessments (Table 1, Table 2, and Table 3).  Screening assessments can occur 
on multiple days, provided they are within the Screening period.  The Investigator will determine 
whether patients meet eligibility criteria and will collect the demographic and medical data 
permitting full characterization of the patient. 
4.1.3 
Single-Dose Phase 
Eligible patients will be randomized in a 3:1 ratio to either active or placebo treatment at the 
Baseline visit.  Patients will receive a single IT treatment.  Within 1 week prior to performing the 
lumbar puncture, a blood sample will be tested locally for platelet count and prothrombin time to 
confirm that it is safe to proceed with the lumbar puncture (Section 8.2.6).  Other predose 
assessments may also be performed up to 1 week prior to dosing, as specifically listed in Table 1 
and Table 2.  
Cohorts will be dosed sequentially, following a safety review by the Dose Escalation Committee: 
• 2 mg Cohort (N=12): WVE-120102 or placebo single-dose IT injection. 
• 4 mg Cohort (N=12): WVE-120102 or placebo single-dose IT injection. 
• 8 mg Cohort (N=12): WVE-120102 or placebo single-dose IT injection. 
• 16 mg Cohort (N=12): 
WVE-120102 or placebo single-dose IT injection. 
• 32 mg Cohort (N=12): 
WVE-120102 or placebo single-dose IT injection.  
Dosing to all cohorts will be initiated in a staggered manner as noted in Figure 1.   
Two sentinel patients in each cohort (1 placebo and 1 WVE-120102) will be dosed and will then 
remain in the clinic for approximately 48 hours postdose.  If either sentinel patient experiences 
an SAE during that period, the safety data will be reviewed by the SMC to determine the next 
steps (Section 4.3.2).  If the sentinel patients do not experience an SAE during that period, the 
remaining 10 patients (2 placebo and 8 WVE-120102) may be dosed in a sequential fashion.  
Immediately after study drug administration, all patients should be ambulatory and active for 
approximately 30 minutes postdose.  The Investigator should note any subtle weakness or fatigue 
during this period.  Formal physical examinations targeting the neurological system will be 
performed at the time points noted in the Schedule of Assessments (Table 1 and Table 2).  Any 
postdose SAEs will be monitored until resolution.   
Sentinel patients will remain in the clinic and undergo assessments throughout the 48-hour 
postdose period.   Day 3 procedures will be performed prior to discharge.  Non-sentinel patients 
will remain in the clinic for a minimum of 24 hours postdose for assessments (Table 1 and Table 
2).  For patients in the 2, 4, 8, and 16 mg cohorts, additional assessments of safety, PK, PD, and 
clinical effects will be performed through the 8-week postdose washout period for all patients. 
For patients in the 32 mg cohort, additional assessments of safety, PK, PD, and clinical effects 
will be performed through 4 weeks after the first dose.  


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Clinical Study Protocol: WVE-HDSNP2-001 
 
17 September 2019 Amendment 2.0 
 
 
Page 28 
The decision regarding escalation to the next planned dose, intermediate dose, extension of the 
current dose level, selection of a higher dose, and if a cohort can proceed to multiple dosing will 
be made by an appointed Dose Escalation Committee, with endorsement from the chairperson of 
the SMC (Section 4.3.3).  Treatment-emergent AEs that meet the Stopping Criteria will be 
reviewed by the unblinded SMC (Section 4.3.4).  In addition, the SMC will review unblinded, 
aggregate safety data (Section 4.3.1).   
4.1.4 
Multiple-Dose Phase 
The Dose Escalation Committee will determine if a dose cohort can proceed to multiple dosing. 
Patients who participated in the single-dose portion of the study must complete the 8-week 
washout period (2, 4, 8, and 16 mg cohorts) or 4-week follow-up period (32 mg cohort) before 
receiving additional doses. Patients will visit the clinic on Day 56 (2, 4, 8, and 16 mg cohorts) or 
Day 28 (32 mg cohort only) for the first of 3 monthly IT doses (Days 56, 84 and 112 or Days 28, 
56, and 84) (Table 1 and Table 2).   
Patients who are enrolled into the multiple-dose portion of the study only will undergo the 
Prescreening (Visit 1) and Screening (Visit 2) visits.  Patients in the 2, 4, 8, and 16 mg cohorts 
will not participate in Visits 3 through 7 and will receive their first dose on Visit 8. Patients will 
return for 2 additional doses (Visit 9 and Visit 10) and for all follow-up visits (Table 3). Patients 
in the 32 mg cohort will not participate in Visits 3 through 6 and will receive their first dose on 
Visit 7. Patients will return for two additional doses (Visit 8 and Visit 9) and for all follow-up 
visits (Table 3).    
Within 1 week prior to each lumbar puncture, a blood sample will be tested locally for platelet 
count and prothrombin time to confirm that it is safe to proceed with the lumbar puncture 
(Section 8.2.6).  Other predose assessments may also be performed up to 1 week prior to dosing, 
as specifically listed in Table 1, Table 2, and Table 3.  
Immediately after study drug administration, all patients should be ambulatory and active for 
approximately 30 minutes postdose.  The Investigator should note any subtle weakness or fatigue 
during this period.  Formal physical examinations targeting the neurological system, will be 
performed at the time points noted in the Schedule of Assessments (Table 1, Table 2, and Table 
3).  Any postdose SAEs will be monitored until resolution.    
Following each dose, patients will remain in the clinic for a minimum of 4 hours postdose for 
safety monitoring.  In addition, patients will be assessed for safety, PK, PD, and clinical effects.   
The SMC will periodically review unblinded, aggregate safety data (Section 4.3.1).   
4.1.5 
Follow-up Phase 
All patients are required to complete follow-up assessments, including evaluations of safety, PD, 
PK, clinical effects, and MRI.  Patients will be followed-up for 14 weeks after the last dose 
(Week 30 for the 2, 4, 8, and 16 mg cohorts and Week 26 for the 32 mg cohort).  An early 
termination (ET) visit will be required in the event of early withdrawal.  


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Clinical Study Protocol: WVE-HDSNP2-001 
 
17 September 2019 Amendment 2.0 
 
 
Page 29 
4.2 
SCHEDULE OF ASSESSMENTS 
The schedule of assessments for patients participating in the single and multiple dose portions of 
the study is presented in Table 1 (2, 4, 8, and 16 mg cohorts) and Table 2 (32 mg cohort).  The 
Schedule of Assessments for patients participating in the multiple-dose portion of the study only 
is presented in Table 3. 
At each relevant time point, the UHDRS, PBA-s, and Columbia-Suicide Severity Rating 
Scale (C-SSRS) should be performed prior to other assessments.    


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Clinical Study Protocol: WVE-HDSNP2-001 
 
17 September 2019 Amendment 2.0 
 
 
Page 30 
 
Table 1 
Schedule of Assessments for Patients Participating in Both the Single and Multiple Dose Portions of the Study (2, 4, 8, and 16 mg 
Cohorts) 
 
Pre-
Screen-
inga 
Screen-
ingb 
Double-Blind Treatment  
(Single Dose) 
Sentinel 
Patient 
Follow-
up  
Follow-up 
Double-Blind 
Treatment  
(Multiple Dose) 
Follow-up 
Visit 
1 
2 
3 
4 
5 
6 
7  
8c 
9 
10 
11 or 
ET 
12 
13 
Week 
 
-4 to 0 
0 
0 
0 
2 
4 
8 
12 
16 
20 
28 
30 
Study Day 
(Visit Window, if applicable) 
Event/Assessment 
 
Day  
-28 to -
1 
Day -6 to 
Day 1 
(Baseline)  
Day 2 
(24 ± 4h 
postdose) 
Day 3 
(48 ± 4h 
postdose) 
Day 
14 
(±2) 
Day 
28  
(±3) 
Day 
56 
(±3) 
Day 
84 
(±3) 
Day 
112 
(±3) 
Day 140 
or ET 
(±5) 
Day 
196 
(±5) 
Day 210 
(EOS) 
(±5)d 
Predose/Non-dosing Days 
 
 
 
 
 
 
 
 
 
 
 
 
 
Patient informed consent form (ICF) Signed 
X 
X 
 
 
 
 
 
 
 
 
 
 
 
Inclusion/exclusion criteria 
 
X 
X 
 
 
 
 
X 
 
 
 
 
 
Medical history and demographics 
 
X 
 
 
 
 
 
 
 
 
 
 
 
Physical examinatione 
 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
 
C-SSRSf, 
 
X 
X* 
 
 
X 
X 
X* 
X* 
X* 
X 
 
 
UHDRS 
 
X 
X 
 
 
X 
X 
X 
X 
X 
X 
 
 
PBA-s 
 
X 
X 
 
 
X 
X 
X 
X 
X 
X 
 
 
Drug screeng 
 
X 
 
 
 
 
 
 
 
 
 
 
 
Urine pregnancy testh 
 
 
X* 
 
 
 
 
X* 
X* 
X* 
 
Adverse event monitoring 
 
X 
X* 
Xq 
Xq 
X 
X 
X* 
X* 
X* 
X 
X 
X 
Prior and concomitant medications 
 
X 
X* 
Xq 
Xq 
X 
X 
X* 
X* 
X* 
X 
X 
X 
3T MRI of the brain (without contrast)  
 
X 
 
 
 
 
X 
 
 
 
X 
 
 
Vital signsi 
 
X 
X* 
X 
X 
X 
X 
X* 
X* 
X* 
X 
X 
 
12-lead ECGj 
 
X 
 
 
 
 
X 
 
 
 
 
 
 
Serum pregnancy testh 
 
X 
 
 
 
 
 
 
 
 
 
 
 
Clinical laboratory testsk 
 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
 
Hepatitis (HBsAg, HCV)  
 
X 
 
 
 
 
 
 
 
 
 
 
 
Blood sample for Prescreening assaysl 
X 
 
 
 
 
 
 
 
 
 
 
 
 
Blood sample for PKm 
 
 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
 
Blood sample for immunogenicityn 
 
 
X 
 
 
 
X 
X 
X 
X 
X 
 
 
CSF sample for safety, exposure, and PDo 
 
 
X* 
 
 
 
X 
X* 
X* 
X* 
XR 
X 
 
Randomization 
 
 
X 
 
 
 
 
 
 
 
 
 
 
Study drug administration via IT injectionp 
 
 
X 
 
 
 
 
X 
X 
X 
 
 
 
Postdose on Dosing Days  
 
 
 
 
 
 
 
 
 
 
 
 
 
Physical examinatione 
 
 
X 
 
 
 
 
X 
X 
X 
 
 
 


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Wave Life Sciences Ltd. 
Clinical Study Protocol: WVE-HDSNP2-001 
 
17 September 2019 Amendment 2.0 
 
 
Page 31 
 
Pre-
Screen-
inga 
Screen-
ingb 
Double-Blind Treatment  
(Single Dose) 
Sentinel 
Patient 
Follow-
up  
Follow-up 
Double-Blind 
Treatment  
(Multiple Dose) 
Follow-up 
Visit 
1 
2 
3 
4 
5 
6 
7  
8c 
9 
10 
11 or 
ET 
12 
13 
Week 
 
-4 to 0 
0 
0 
0 
2 
4 
8 
12 
16 
20 
28 
30 
Study Day 
(Visit Window, if applicable) 
Event/Assessment 
 
Day  
-28 to -
1 
Day -6 to 
Day 1 
(Baseline)  
Day 2 
(24 ± 4h 
postdose) 
Day 3 
(48 ± 4h 
postdose) 
Day 
14 
(±2) 
Day 
28  
(±3) 
Day 
56 
(±3) 
Day 
84 
(±3) 
Day 
112 
(±3) 
Day 140 
or ET 
(±5) 
Day 
196 
(±5) 
Day 210 
(EOS) 
(±5)d 
Adverse event monitoringq 
 
 
X 
 
 
 
 
X 
X 
X 
 
 
 
Clinical laboratory testsk 
 
 
X 
 
 
 
 
X 
X 
X 
 
 
 
Concomitant medications 
 
 
X 
 
 
 
 
X 
X 
X 
 
 
 
Vital signsi 
 
 
X 
 
 
 
 
X 
X 
X 
 
 
 
Blood samples for PKm 
 
 
X 
 
 
 
 
 
 
X 
 
 
 
Study completion 
 
 
 
 
 
 
 
 
 
 
 
 
X 
Abbreviations: AE = adverse event; CAG = cytosine-adenine-guanine; CSF = cerebrospinal fluid; C-SSRS = Columbia-Suicide Severity Rating Scale; ECG = electrocardiogram; EOS = end of study; 
ET = early termination; h = hour; HBsAg = hepatitis B surface antigen; HCV = hepatitis C virus; ICF = informed consent form; IT = intrathecal; MRI = magnetic resonance imaging; PBA-s = Short 
Problem Behaviors Assessment; PD = pharmacodynamics; PK = pharmacokinetics; SNP= single nucleotide polymorphism; SNP2 = SNP rs362331 T= thymine; UHDRS = Unified Huntington’s Disease 
Rating Scale.  
Note: The UHDRS, PBA-s, and C-SSRS should be completed prior to any other assessments.  Assessments completed predose on Day 1 are considered Baseline.  
*For each dosing visit (Day 1/Visit 3 [single dose phase]; Day 56/Visit 8; Day 84/Visit 9; and Day 112/Visit 10 [multiple dose phase]), these predose procedures must be performed predose on 
the day of dosing.  All other predose assessments (inclusion/exclusion criteria [Day 1/Visit 3 and Day 56/Visit 8 only], physical examination, UHDRS, PBA-S, clinical laboratory tests 
[including a blood sample to confirm platelet count and prothrombin clotting time], blood sample for PK, and blood sample for immunogenicity) may be performed up to a week prior to 
dosing.  
a Prescreening is only required for patients who do not have documented heterozygosity for SNP rs362331 (SNP2) with the T variant on the same allele as the cytosine-adenine-guanine (CAG) triplet 
expansion. Prescreening can happen anytime before Screening.  It is anticipated that it will take at least 6 weeks to process the results of this testing. 
b Screening assessments can occur on multiple days, provided they are within the 4-week Screening period.   
c There is a minimum washout period of 8 weeks between the first dose and the start of multiple dosing. However, due to the timing of Dose Escalation Committee review to determine if the multiple 
dose phase can proceed for a dose cohort, the duration between the Week 4 visit and Week 8 visit may be longer than 4 weeks.  Investigators will be notified when the Visit 8/Week 8 visit can occur.  
During this time, a telephone contact for safety follow-up should be performed monthly, at a minimum. 
d Follow-up Day 210 Visit for AE monitoring can be a telephone call. 
e At Screening, physical examination will include (but is not limited to) an examination of skin; head, eyes, ears, nose, throat; respiratory; cardiovascular; gastrointestinal; endocrine, metabolic; blood, 
lymphatic; musculoskeletal, psychiatric, and neurologic systems.  Predose and on Visits 6 (Day 14), 7 (Day 28), 11 (Day 140), and 12 (196) the physical exam must include (at a minimum) head, 
eyes, ears, nose, throat, respiratory, cardiovascular, gastrointestinal, musculoskeletal, psychiatric, and neurologic systems.  Other systems should be evaluated as appropriate. After study drug 
administration, a targeted physical exam, including the neurologic system (with special attention to the motor system, upper and lower extremity measures of strength, tone, reflexes, and ambulation), 
will be performed 30 minutes (±5 minutes), 1, 2, 3, and 4 hours (±15 minutes) postdose, with an additional exam 24 hours (±4 hours)  after the first dose.  The sentinel patients in each cohort will 
also repeat this exam at 8 hours (±30 minutes), 12 hours (±2 hours), 24, and 48 hours (±4 hours).   
f C-SSRS baseline/screening version will be performed at the Screening Visit, and the ‘since last visit’ version will be performed at all other noted visits. 
g Urine drug screen for selected drugs of abuse.  
h For female patients of childbearing potential, a negative serum pregnancy test must be documented at the Screening Visit.  On dosing days, a negative urine pregnancy test must be documented 
predose.  


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Clinical Study Protocol: WVE-HDSNP2-001 
 
17 September 2019 Amendment 2.0 
 
 
Page 32 
i 
Vital signs include blood pressure, pulse, temperature, weight, and height.  On dosing days blood pressure and pulse will be measured at the following time points:  ≤30 minutes predose and 1, 2, and 
4 hours (±15 minutes) postdose. Sentinel patients will also have blood pressure and pulse measured 12 hours (±2 hours), 24 and 48 hours (±4 hours) postdose.  Weight and temperature will only be 
measured predose on dosing days.  Height will be measured at baseline only.  Blood pressure (systolic and diastolic), temperature, and pulse should be measured after patient has been resting quietly 
for ≥3 minutes.   
j 
Electrocardiogram recordings will be obtained in triplicate in the supine position after the patient has rested comfortably for ≥5 minutes.    
k Clinical laboratory safety testing, including clinical chemistry, hematology, and urinalysis.  Within 1 week prior to lumbar puncture, a blood sample will be tested to confirm platelet count and 
prothrombin clotting time.  Clinical laboratory safety testing will be performed predose on all dosing days.  On Day 1 only, samples for clinical chemistry and hematology measures (CBC, 
complement, and fibrinogen only) will also be collected at 1 and 4 hours (±15 minutes), and 24 hours (±4 hours) postdose, and for sentinel patients only, 48 hours (±4 hours).  On all non-dosing days, 
clinical laboratory safety testing will be performed just once. On Day 196, only platelet count and prothrombin clotting time need to be assessed (prior to CSF sample).  Urinalysis will be performed 
predose on all dosing days, and once on all other days noted 
l 
Blood samples for the Prescreening assays to determine eligibility. 
m On Day 1, sentinel patients will have blood samples for PK analysis collected predose, 30 minutes (±5 minutes), 1, 2, 4 hours (±15 minutes), 12 hours (±2 hours), 24 hours  and 48 hours (±4 hours) 
postdose. All other patients will have blood samples for PK analysis collected predose, and 30 minutes (±5 minutes), 1, 2, 4 hours (±15 minutes), 12 hours (±2 hours) and 24 hours (±4 hours) 
postdose on Day 1.  On Day 112, all patients will have blood samples collected for PK analysis predose and 1, 2, and 4 hours (±15 minutes) postdose.  On all other dosing days, PK samples will be 
collected predose only. On non-dosing days noted, PK will be collected once.  
n Blood sample for assessment of antibodies to WVE-120102 will be obtained predose (within 1 week prior to injection) or on non-dosing days, as indicated. 
o On dosing days, CSF sample will be collected ≤15 minutes prior to study drug administration.  No more than 10 mL of CSF total should be collected. CSF samples must be tested locally for safety 
for the following parameters: total protein, glucose and cell counts (white blood cell counts with differential) on all noted visits.  
p Immediately after study drug administration, patients should be active for approximately 30 minutes postdose.  
q All patients will remain in the clinic and have AEs and concomitant medications recorded through 24 hours postdose. In addition, 2 sentinel patients will remain in the clinic and have AEs and 
concomitant medications recorded throughout the 48-hour postdose period.  
r 
This lumbar puncture is optional for patients who terminate early. 
 


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Clinical Study Protocol: WVE-HDSNP2-001 
 
17 September 2019 Amendment 2.0 
 
 
Page 33 
Table 2 
Schedule of Assessments for Patients Participating in Both the Single and Multiple Dose Portions of the Study (32 mg Cohort) 
 
Pre-
Screen-
inga 
Screen-
ingb 
Double-Blind Treatment  
(Single Dose) 
Sentinel 
Patient 
Follow-
up  
Follow-
up 
Double-Blind 
Treatment  
(Multiple Dose) 
Follow-up 
Visit 
1 
2 
3 
4 
5 
6 
7c 
8 
9 
10 or ET 
11 
12 
Week 
 
-4 to 0 
0 
0 
0 
2 
4 
8 
12 
16 
24 
26 
Study Day 
(Visit Window, if applicable) 
Event/Assessment 
 
Day  
-28 to -
1 
Day -6 to 
Day 1 
(Baseline)  
Day 2 
(24 ± 4h 
postdose) 
Day 3 
(48 ± 4h 
postdose) 
Day 14 
(±2) 
Day 
28  
(±3) 
Day 
56 
(±3) 
Day 
84 
(±3) 
Day 112 
or ET 
(±5) 
Day 
168 
(±5) 
Day 182(EOS) 
(±5)d 
Predose/Non-dosing Days 
Patient informed consent form (ICF) Signed 
X 
X 
 
 
 
 
 
 
 
 
 
 
Inclusion/exclusion criteria 
 
X 
X 
 
 
 
X 
 
 
 
 
 
Medical history and demographics 
 
X 
 
 
 
 
 
 
 
 
 
 
Physical examinatione 
 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
 
C-SSRSf, 
 
X 
X* 
 
 
X 
X* 
X* 
X* 
X 
 
 
UHDRS 
 
X 
X 
 
 
X 
X 
X 
X 
X 
 
 
PBA-s 
 
X 
X 
 
 
X 
X 
X 
X 
X 
 
 
Drug screeng 
 
X 
 
 
 
 
 
 
 
 
 
 
Urine pregnancy testh 
 
 
X* 
 
 
 
X* 
X* 
X* 
 
Adverse event monitoring 
 
X 
X* 
Xq 
Xq 
X 
X* 
X* 
X* 
X 
X 
X 
Prior and concomitant medications 
 
X 
X* 
Xq 
Xq 
X 
X* 
X* 
X* 
X 
X 
X 
3T MRI of the brain (without contrast)  
 
X 
 
 
 
 
X 
 
 
X 
 
 
Vital signsi 
 
X 
X* 
X 
X 
X 
X* 
X* 
X* 
X 
X 
 
12-lead ECGj 
 
X 
 
 
 
X 
 
 
 
 
 
 
Serum pregnancy testh 
 
X 
 
 
 
 
 
 
 
 
 
 
Clinical laboratory testsk 
 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
 
Hepatitis (HBsAg, HCV)  
 
X 
 
 
 
 
 
 
 
 
 
 
Blood sample for Prescreening assaysl 
X 
 
 
 
 
 
 
 
 
 
 
 
Blood sample for PKm 
 
 
X 
X 
X 
X 
X 
X 
X 
X 
X 
 
Blood sample for immunogenicityn 
 
 
X 
 
 
 
X 
X 
X 
X 
 
 
CSF sample for safety, exposure, and PDo 
 
 
X* 
 
 
 
X* 
X* 
X* 
Xr 
X 
 
Randomization 
 
 
X 
 
 
 
 
 
 
 
 
 
Study drug administration via IT injectionp 
 
 
X 
 
 
 
X 
X 
X 
 
 
 
Postdose on Dosing Days  
Physical examinatione 
 
 
X 
 
 
 
X 
X 
X 
 
 
 
Adverse event monitoringq 
 
 
X 
 
 
 
X 
X 
X 
 
 
 


WVE-120102 
 
Wave Life Sciences Ltd. 
Clinical Study Protocol: WVE-HDSNP2-001 
 
17 September 2019 Amendment 2.0 
 
 
Page 34 
 
Pre-
Screen-
inga 
Screen-
ingb 
Double-Blind Treatment  
(Single Dose) 
Sentinel 
Patient 
Follow-
up  
Follow-
up 
Double-Blind 
Treatment  
(Multiple Dose) 
Follow-up 
Visit 
1 
2 
3 
4 
5 
6 
7c 
8 
9 
10 or ET 
11 
12 
Week 
 
-4 to 0 
0 
0 
0 
2 
4 
8 
12 
16 
24 
26 
Study Day 
(Visit Window, if applicable) 
Event/Assessment 
 
Day  
-28 to -
1 
Day -6 to 
Day 1 
(Baseline)  
Day 2 
(24 ± 4h 
postdose) 
Day 3 
(48 ± 4h 
postdose) 
Day 14 
(±2) 
Day 
28  
(±3) 
Day 
56 
(±3) 
Day 
84 
(±3) 
Day 112 
or ET 
(±5) 
Day 
168 
(±5) 
Day 182(EOS) 
(±5)d 
Clinical laboratory testsk 
 
 
X 
 
 
 
X 
X 
X 
 
 
 
Concomitant medications 
 
 
X 
 
 
 
X 
X 
X 
 
 
 
Vital signsi 
 
 
X 
 
 
 
X 
X 
X 
 
 
 
Blood samples for PKm 
 
 
X 
 
 
 
 
 
X 
 
 
 
Study completion 
 
 
 
 
 
 
 
 
 
 
 
X 
Abbreviations: AE = adverse event; CAG = cytosine-adenine-guanine; CSF = cerebrospinal fluid; C-SSRS = Columbia-Suicide Severity Rating Scale; ECG = electrocardiogram; EOS = end of study; 
ET = early termination; h = hour; HBsAg = hepatitis B surface antigen; HCV = hepatitis C virus; ICF = informed consent form; IT = intrathecal; MRI = magnetic resonance imaging; PBA-s = Short 
Problem Behaviors Assessment; PD = pharmacodynamics; PK = pharmacokinetics; SNP= single nucleotide polymorphism; SNP2 = SNP rs362331 T= thymine; UHDRS = Unified Huntington’s Disease 
Rating Scale.  
Note: The UHDRS, PBA-s, and C-SSRS should be completed prior to any other assessments.  Assessments completed predose on Day 1 are considered Baseline.  
*For each dosing visit (Day 1/Visit 3 [single dose phase]; Day 28/Visit 7; Day 56/Visit 8; and Day 84/Visit 9 [multiple dose phase]), these predose procedures must be performed predose on the 
day of dosing.  All other predose assessments (inclusion/exclusion criteria [Day 1/Visit 3 and Day 28/Visit 7 only], physical examination, UHDRS, PBA-S, clinical laboratory tests [including a 
blood sample to confirm platelet count and prothrombin clotting time], blood sample for PK, and blood sample for immunogenicity) may be performed up to a week prior to dosing.  
a Prescreening is only required for patients who do not have documented heterozygosity for SNP rs362331 (SNP2) with the T variant on the same allele as the cytosine-adenine-guanine (CAG) triplet 
expansion. Prescreening can happen anytime before Screening.  It is anticipated that it will take at least 6 weeks to process the results of this testing. 
b Screening assessments can occur on multiple days, provided they are within the 4-week Screening period.   
c There is a minimum follow-up period of 4 weeks between the first dose and the start of multiple dosing. However, due to the timing of Dose Escalation Committee review to determine if the multiple 
dose phase can proceed for a dose cohort, the duration between the Week 4 visit and Week 7 visit may be longer than 4 weeks.  Investigators will be notified when the Visit 7/Week 4 visit can occur.  
During this time, a telephone contact for safety follow-up should be performed monthly, at a minimum. 
d Follow-up Day 182 Visit for AE monitoring can be a telephone call. 
e At Screening, physical examination will include (but is not limited to) an examination of skin; head, eyes, ears, nose, throat; respiratory; cardiovascular; gastrointestinal; endocrine, metabolic; blood, 
lymphatic; musculoskeletal, psychiatric, and neurologic systems.  Predose and on Visits 6 (Day 14), 10 (Day 112), and 11 (168) the physical exam must include (at a minimum) head, eyes, ears, 
nose, throat, respiratory, cardiovascular, gastrointestinal, musculoskeletal, psychiatric, and neurologic systems.  Other systems should be evaluated as appropriate. After study drug administration, a 
targeted physical exam, including the neurologic system (with special attention to the motor system, upper and lower extremity measures of strength, tone, reflexes, and ambulation), will be 
performed 30 minutes (±5 minutes), 1, 2, 3, and 4 hours (±15 minutes) postdose, with an additional exam 24 hours (±4 hours)  after the first dose.  The sentinel patients in each cohort will also repeat 
this exam at 8 hours (±30 minutes), 12 hours (±2 hours), 24, and 48 hours (±4 hours).   
f C-SSRS baseline/screening version will be performed at the Screening Visit, and the ‘since last visit’ version will be performed at all other noted visits. 
g Urine drug screen for selected drugs of abuse.  
h For female patients of childbearing potential, a negative serum pregnancy test must be documented at the Screening Visit.  On dosing days, a negative urine pregnancy test must be documented 
predose.  
i 
Vital signs include blood pressure, pulse, temperature, weight, and height.  On dosing days blood pressure and pulse will be measured at the following time points: ≤30 minutes predose and 1, 2, and 
4 hours (±15 minutes) postdose. Sentinel patients will also have blood pressure and pulse measured 12 hours (±2 hours), 24 and 48 hours (±4 hours) postdose.  Weight and temperature will only be 


WVE-120102 
 
Wave Life Sciences Ltd. 
Clinical Study Protocol: WVE-HDSNP2-001 
 
17 September 2019 Amendment 2.0 
 
 
Page 35 
measured predose on dosing days.  Height will be measured at baseline only.  Blood pressure (systolic and diastolic), temperature, and pulse should be measured after patient has been resting quietly 
for ≥3 minutes.   
j 
Electrocardiogram recordings will be obtained in triplicate in the supine position after the patient has rested comfortably for ≥5 minutes.    
k Clinical laboratory safety testing, including clinical chemistry, hematology, and urinalysis.  Within 1 week prior to lumbar puncture, a blood sample will be tested to confirm platelet count and 
prothrombin clotting time.  Clinical laboratory safety testing will be performed predose on all dosing days.  On Day 1 only, samples for clinical chemistry and hematology measures (CBC, 
complement, and fibrinogen only) will also be collected at 1 and 4 hours (±15 minutes), and 24 hours (±4 hours) postdose, and for sentinel patients only, 48 hours (±4 hours).  On all non-dosing days, 
clinical laboratory safety testing will be performed just once. On Day 168, only platelet count and prothrombin clotting time need to be assessed (prior to CSF sample).  Urinalysis will be performed 
predose on all dosing days, and once on all other days noted 
l 
Blood samples for the Prescreening assays to determine eligibility. 
m On Day 1, sentinel patients will have blood samples for PK analysis collected predose, 30 minutes (±5 minutes), 1, 2, 4 hours (±15 minutes), 12 hours (±2 hours), 24 hours  and 48 hours (±4 hours) 
postdose. All other patients will have blood samples for PK analysis collected predose, and 30 minutes (±5 minutes), 1, 2, 4 hours (±15 minutes), 12 hours (±2 hours) and 24 hours (±4 hours) 
postdose on Day 1.  On Day 84, all patients will have blood samples collected for PK analysis predose and 1, 2, and 4 hours (±15 minutes) postdose.  On all other dosing days, PK samples will be 
collected predose only. On non-dosing days noted, PK will be collected once.  
n Blood sample for assessment of antibodies to WVE-120102 will be obtained predose (within 1 week prior to injection) or on non-dosing days, as indicated. 
o On dosing days, CSF sample will be collected ≤15 minutes prior to study drug administration.  No more than 10 mL of CSF total should be collected. CSF samples must be tested locally for safety 
for the following parameters: total protein, glucose and cell counts (white blood cell counts with differential) on all noted visits.  
p Immediately after study drug administration, patients should be active for approximately 30 minutes postdose.  
q All patients will remain in the clinic and have AEs and concomitant medications recorded through 24 hours postdose. In addition, 2 sentinel patients will remain in the clinic and have AEs and 
concomitant medications recorded throughout the 48-hour postdose period.  
r 
This lumbar puncture is optional for patients who terminate early.


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Table 3 
Schedule of Assessments for Treatment-Naïve Patients in the Multiple-Dose Portion of the Study 
 
Pre-Screen-
inga 
Screen-ingb 
Double-Blind Treatment  
(Multiple Dose) 
Follow-up 
Visit 
1 
2 
8 (7q) 
9 (8q) 
10 (9q) 
11 (10q) or 
ET 
12 (11q) 
13 (12q) 
Week 
 
-4 to 0 
8 (4q) 
12 (8q) 
16 (12q) 
20 (16q) 
28 (24q) 
30 (26q) 
Study Day 
(Visit Window, if applicable) 
Event/Assessment 
 
Day  
-28 to -1 
Day 56  
(28q) 
(±3) 
(Baseline) 
Day 84 
(56q) 
(±3) 
Day 112 
(84q) 
(±3) 
Day 140 
(112q) or ET 
(±5) 
Day 196 
(168q) 
(±5) 
Day 210c 
(182q) 
(EOS) 
(±5) 
Predose/Non-dosing Days 
 
 
 
 
 
 
 
 
Patient informed consent form (ICF) Signed 
X 
X 
 
 
 
 
 
 
Inclusion/exclusion criteria 
 
X 
X 
 
 
 
 
 
Medical history and demographics 
 
X 
 
 
 
 
 
 
Physical examinationd 
 
X 
X 
X 
X 
X 
X 
 
C-SSRSe 
 
X 
X* 
X* 
X 
X* 
 
 
UHDRS 
 
X 
X 
X 
X 
X 
 
 
PBA-s 
 
X 
X 
X 
X 
X 
 
 
Drug screenf 
 
X 
 
 
 
 
 
 
Urine pregnancy testg 
 
 
X* 
X* 
X* 
 
 
 
Adverse event monitoring 
 
X 
X* 
X* 
X* 
X 
X 
X 
Prior and concomitant medications 
 
X 
X* 
X* 
X* 
X 
X 
X 
3T MRI of the brain (without contrast) 
 
X 
 
 
 
X 
 
 
Vital signsh 
 
X 
X* 
X* 
X* 
X 
X 
 
12-lead ECGi 
 
X 
 
 
 
 
 
 
Serum pregnancy testg 
 
X 
 
 
 
 
 
 
Clinical laboratory testsj 
 
X 
X 
X 
X 
X 
X 
 
Hepatitis (HBsAg, HCV) 
 
X 
 
 
 
 
 
 
Blood sample for Prescreening assaysk 
X 
 
 
 
 
 
 
 
Blood sample for PKl 
 
 
X 
X 
X 
X 
X 
 
Blood sample for immunogenicitym 
 
 
X 
X 
X 
X 
 
 
CSF sample for safety, exposure, and PDn 
 
 
X* 
X* 
X* 
Xp 
X 
 
Treatment Assignment 
 
 
X 
 
 
 
 
 
Study drug administration via IT injectiono 
 
 
X 
X 
X 
 
 
 
Postdose on Dosing Days  
- 
 
 
 
 
 
 
 
Physical examinationd 
 
 
X 
X 
X 
 
 
 
Adverse event monitoring 
 
 
X 
X 
X 
 
 
 
Clinical laboratory testsj 
 
 
X 
X 
X 
 
 
 


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Pre-Screen-
inga 
Screen-ingb 
Double-Blind Treatment  
(Multiple Dose) 
Follow-up 
Visit 
1 
2 
8 (7q) 
9 (8q) 
10 (9q) 
11 (10q) or 
ET 
12 (11q) 
13 (12q) 
Week 
 
-4 to 0 
8 (4q) 
12 (8q) 
16 (12q) 
20 (16q) 
28 (24q) 
30 (26q) 
Study Day 
(Visit Window, if applicable) 
Event/Assessment 
 
Day  
-28 to -1 
Day 56  
(28q) 
(±3) 
(Baseline) 
Day 84 
(56q) 
(±3) 
Day 112 
(84q) 
(±3) 
Day 140 
(112q) or ET 
(±5) 
Day 196 
(168q) 
(±5) 
Day 210c 
(182q) 
(EOS) 
(±5) 
Concomitant medications 
 
 
X 
X 
X 
 
 
 
Vital signsh 
 
 
X 
X 
X 
 
 
 
Blood samples for PKl 
 
 
 
 
X 
 
 
 
Study completion 
 
 
 
 
 
 
 
X 
Abbreviations: AE = adverse event; CAG = cytosine-adenine-guanine; CSF = cerebrospinal fluid; C-SSRS = Columbia-Suicide Severity Rating Scale; ECG = electrocardiogram; EOS = end of study; 
ET = early termination; h = hour; HBsAg = hepatitis B surface antigen; HCV = hepatitis C virus; ICF = informed consent form; IT = intrathecal; MRI = magnetic resonance imaging; PBA-s = Short 
Problem Behaviors Assessment; PD = pharmacodynamics; PK = pharmacokinetics; SNP= single nucleotide polymorphism; SNP2 = SNP rs362331 T= thymine; UHDRS = Unified Huntington’s 
Disease Rating Scale.  
Note: The UHDRS, PBA-s, and C-SSRS should be completed prior to any other assessments.  Assessments completed predose on Day 56 are considered Baseline.  
Patients who are treatment-naïve for the multiple-dose portion of the study will undergo Prescreening and Screening, and then receive their first dose on Day 56 (Visit 56).   
*For each dosing visit during the multiple dose phase, these predose procedures must be performed predose on the day of dosing.  All other predose assessments (inclusion/exclusion 
criteria [Day 56/Visit 8 for 2, 4, 8, and 16 mg cohorts or Day 28/Visit 7 for 32 mg cohort], physical examination, UHDRS, PBA-S, clinical laboratory tests [including a blood sample to 
confirm platelet count and prothrombin clotting time], blood sample for PK, and blood sample for immunogenicity) may be performed up to a week prior to dosing. 
a Prescreening is only required for patients who do not have documented heterozygosity for SNP rs362331 (SNP2) with the T variant on the same allele as the cytosine-adenine-guanine (CAG) 
triplet expansion.  Prescreening can happen anytime before Screening.  It is anticipated that it will take at least 6 weeks to process the results of this testing. 
b Screening assessments can occur on multiple days, provided they are within the 4-week Screening period.   
c Follow-up Day 210 Visit (Day 182 Visit for the 32 mg cohort) for AE monitoring can be a telephone call. 
d At Screening, physical examination will include (but is not limited to) an examination of skin; head, eyes, ears, nose, throat; respiratory; cardiovascular; gastrointestinal; endocrine, metabolic; 
blood, lymphatic; musculoskeletal, psychiatric, and neurologic systems.  Predose and on Visit 11/Day 140  and Visit 12/Day 196 for the 2, 4, 8, and 16 mg cohorts and Visit 10/Day 112 and Visit 
11/Day 168 for the 32 mg cohort, the physical exam must include (at a minimum) head, eyes, ears, nose, throat, respiratory, cardiovascular, gastrointestinal, musculoskeletal, psychiatric, and 
neurologic systems.  Other systems should be evaluated as appropriate. After study drug administration, a targeted physical exam, including the neurologic system (with special attention to the 
motor system, upper and lower extremity measures of strength, tone, reflexes, and ambulation), will be performed 30 minutes (±5 minutes), 1, 2, 3, and 4 hours (±15 minutes) postdose.    
e C-SSRS baseline/screening version will be performed at the Screening Visit, and the ‘since last visit’ version will be performed at all other noted visits. 
f Urine drug screen for selected drugs of abuse.  
g For female patients of childbearing potential, a negative serum pregnancy test must be documented at the Screening Visit.  On dosing days, a negative urine pregnancy test must be documented 
predose.  
h Vital signs include blood pressure, pulse, temperature, weight, and height.  On dosing days blood pressure and pulse will be measured at the following time points:  ≤30 minutes predose and 1, 2, 
and 4 hours (±15 minutes) postdose. Weight and temperature will only be measured predose on dosing days.  Height will be measured at baseline only.  Blood pressure (systolic and diastolic), 
temperature, and pulse should be measured after patient has been resting quietly for ≥3 minutes.   
i 
Electrocardiogram recordings will be obtained in triplicate in the supine position after the patient has rested comfortably for ≥5 minutes.    
j 
Clinical laboratory safety testing, including clinical chemistry, hematology, and urinalysis.  Within 1 week prior to lumbar puncture, a blood sample will be tested to confirm platelet count and 
prothrombin clotting time.  Clinical laboratory safety testing will be performed predose on all dosing days. On Day 56 (Day 28 for 32 mg cohort) only, samples for clinical chemistry and 
hematology measures (CBC, complement, and fibrinogen only) will also be collected at 1 and 4 hours (±15 minutes) postdose. On all non-dosing days, clinical laboratory safety testing will be 


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Pre-Screen-
inga 
Screen-ingb 
Double-Blind Treatment  
(Multiple Dose) 
Follow-up 
Visit 
1 
2 
8 (7q) 
9 (8q) 
10 (9q) 
11 (10q) or 
ET 
12 (11q) 
13 (12q) 
Week 
 
-4 to 0 
8 (4q) 
12 (8q) 
16 (12q) 
20 (16q) 
28 (24q) 
30 (26q) 
Study Day 
(Visit Window, if applicable) 
Event/Assessment 
 
Day  
-28 to -1 
Day 56  
(28q) 
(±3) 
(Baseline) 
Day 84 
(56q) 
(±3) 
Day 112 
(84q) 
(±3) 
Day 140 
(112q) or ET 
(±5) 
Day 196 
(168q) 
(±5) 
Day 210c 
(182q) 
(EOS) 
(±5) 
performed just once.  On Day 196, only platelet count and prothrombin clotting time need to be assessed (prior to CSF sample).  Urinalysis will be performed predose on all dosing days, and once 
on all other days noted. 
k Blood samples for the Prescreening assays to determine eligibility. 
l 
On Day 56 (Day 28 for 32 mg cohort), all patients will have blood samples collected for PK analysis predose and 30 minutes (±5 minutes), 1, 2, 4 hours (±15 minutes) postdose.  On Day 112 
(Day 84 for the 32 mg cohort), all patients will have blood samples collected for PK analysis predose and 1, 2, and 4 hours (±15 minutes) postdose.  On all other dosing days, PK samples will be 
collected predose only. On non-dosing days noted, PK will be collected once.  
m Blood sample for assessment of antibodies to WVE-120102 will be obtained predose (within 1 week prior to injection) or on non-dosing days, as indicated. 
n On dosing days, CSF sample will be collected ≤15 minutes prior to study drug administration.  No more than 10 mL of CSF total should be collected.  CSF samples must be tested locally for 
safety for the following parameters: total protein, glucose and cell counts (white blood cell counts with differential) on all noted visits.  
o Immediately after study drug administration, patients should be active for approximately 30 minutes postdose.  
p This lumbar puncture is optional for patients who terminate early.  
q   Visit, week, and day for the 32 mg cohort.  
 
 


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4.3 
SAFETY REVIEWS, STOPPING CRITERIA, AND COMMUNICATION 
PLAN 
Periodic safety reviews, including reviews of treatment-emergent AEs (TEAEs) that meet the 
Stopping Criteria, will be performed by the unblinded, independent SMC (Section 13.1) 
throughout the study, as specified below.  The study can be terminated at any time based on the 
findings of any safety reviews.  
4.3.1 
Safety Monitoring Committee Reviews 
The independent SMC will review unblinded, aggregate safety data periodically and will review 
SAE reports in real time.  Recommendations based on these reviews will be provided to the 
Sponsor.   
The SMC will also review SAEs that occur in the sentinel patients (Section 4.3.2) and TEAEs 
that meet the Stopping Criteria (Section 4.3.4).  In addition, the SMC will review the Dose 
Escalation Committee’s decisions regarding dose escalation and continuation to multiple dosing, 
and the chairperson will endorse the decision.  Neither escalation to the next dose level nor 
continuation to multiple dosing may proceed without review of unblinded data by the SMC and 
subsequent endorsement by the committee Chair.  
Based on any of the safety reviews performed by the SMC, the SMC may recommend stopping 
the study at any time.   
4.3.2 
Safety Review After Sentinel Patients  
The SMC will perform reviews of any SAEs that occur in the sentinel patients.  If either of the 
sentinel patients in a cohort experiences an SAE within 48 hours postdose, all pertinent safety 
data will be provided to the SMC.  No further patients in the cohort will be dosed pending this 
data review.  The SMC will determine if it is safe to proceed with the cohort, or, in conjunction 
with the Dose Escalation Committee, whether an intermediate dose should be selected for the 
remaining patients in that cohort.   
4.3.3 
Dose Escalation and Continuation to Multiple Dosing  
The Dose Escalation Committee will review blinded data to make the decision regarding 
escalation to each subsequent dose and whether a single dose cohort can proceed to multiple 
dosing.  After all patients in the 2, 4, 8, and 16 mg cohorts complete the Day 28 Visit and all 
patients in the 32 mg cohort complete the Day 14 Visit, the Dose Escalation Committee will 
review: 
• Safety data from all patients in the current cohort through the Day 28 visit (2, 4, 8, and 16 mg 
cohorts) or Day 14 (32 mg cohort).   
• Any available safety data from patients in the current cohort through the Day 56 visit (2, 4, 8, 
and 16 mg cohorts) or Day 28 visit (32 mg cohort).  
• Any available safety data from all other cohorts, including those that have proceeded to 
multiple dosing (for the 4, 8,16, and 32 mg dose cohorts, a minimum of 50% of patients in 
the previous cohort must have received at least 2 doses in the multiple dose phase, and all 


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data from the single-dose phase must be reviewed for the current cohort to proceed to 
multiple doses). 
• Treatment-emergent adverse events (TEAEs) that resulted in stopping criteria review 
(Section 4.3.4). 
The Dose Escalation Committee may decide to enroll a larger number of patients in a cohort if 
further information is needed regarding single administration at that dose, provided no suspected 
unexpected serious adverse reactions (SUSARs) have occurred at that dose.  For the next cohort, 
the Dose Escalation Committee may decide to select an intermediate dose, extend the current 
dose level, or select a higher dose.  The high dose of 32 mg will not be exceeded; if the Sponsor 
decides that doses >32 mg should be explored, this will be addressed in a protocol amendment.   
If the Dose Escalation Committee determines that the cohort should not continue to multiple 
dosing, that cohort will end.  Patients will be monitored for safety through 8 weeks postdose.  
The Dose Escalation Committee may identify a lower dose for the subsequent cohort.   
All recommendations will be reviewed by the SMC and endorsed by the chairperson.  Neither 
escalation to the next dose level nor continuation to multiple dosing may proceed without review 
of unblinded data by the SMC and subsequent endorsement by the committee Chair.  Sites will 
be notified rapidly of any decisions made.  
4.3.4 
Stopping Criteria 
4.3.4.1 
Single-dose Phase 
During the single-dose, dose escalation phase, dosing will be suspended: 
1. If a single patient experiences an SAE assessed as related to study drug; 
2. If ≥2 patients experience a TEAE of ≥Grade 3 (using National Cancer Institute Common 
Terminology Criteria for AE [NCI CTCAE] grading) that is assessed as related to study drug; 
or 
3. If ≥4 patients experience a non-serious TEAE of ≥Grade 2 within the Medical Dictionary for 
Regulatory Activities (MedDRA) High Level Group Term (HLGT) Spinal Cord and Nerve 
Root Disorders, assessed as related to study drug. 
Objective stopping criterion (#3) was developed to assess any potential events of neurological 
toxicity that were not otherwise captured via surveillance of serious and severe TEAEs as 
outlined in criteria 1 and 2.  Certain adverse events may be due to the IT administration and are 
therefore exempt from the stopping criteria (discussed below). 
If the stopping criteria are met, the unblinded safety data will be reviewed in a timely manner by 
the SMC to determine if it is safe to proceed with the cohort or if an intermediate dose should be 
selected for the remaining patients in that cohort.  If a determination is made to resume the study, 
information will be submitted to the regulatory authorities in accordance with local regulations 
(e.g., amendment) prior to restarting treatment. 


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Any SUSARs that occur will be reported to regulatory authorities as required.  If a decision is 
made to terminate dosing in a cohort, all Investigators will be informed immediately, as detailed 
in Section 4.3.5. 
Adverse Events That are Exempt from Stopping Criteria 
Intrathecal administration of drugs is known to result in untoward effects such as post-lumbar 
puncture headache, pain, etc24,25.  Given the common occurrence of such side-effects following 
lumbar puncture, it is anticipated that patients may experience AEs temporal to the IT 
administration procedure which are not attributable to study drug24,25.  Therefore, the AE terms 
listed in Table 4 will be exempted from the assessment of Stopping Criteria:   
Table 4 
Adverse Event Preferred Terms Exempted From Stopping Criteria 
Preferred Terms Exempt From Stopping Criteria 
Administration site bruise 
Instillation site bruise 
Catheter site bruise 
Post-lumbar puncture syndrome 
Infusion site bruising 
Post-procedural discomfort 
Infusion site discomfort 
Post-procedural contusion 
Infusion site pain 
Procedural headache 
Injection site bruising 
Procedural pain 
Injection site discomfort 
Traumatic lumbar puncture 
Injection site pain 
 
Note: AEs exempted from the Stopping Criteria include the MedDRA PTs listed above and their associated 
verbatim terms. 
 
While these procedural events are exempt from Stopping Criteria review, they will be recorded 
as AEs as described in Section 9.  In addition, as part of their ongoing review of unblinded safety 
data, the SMC will carefully review the above-referenced, exempted AE terms to ensure there 
are no meaningful imbalances between treatment groups.   
4.3.4.2 
Multiple-dose Phase Stopping Criteria for an Individual Patient 
Dosing will be stopped for an individual patient if: 
1. Patient experiences a serious or intolerable AE that, in the Investigator’s opinion, requires 
study drug discontinuation. 
2. Patient experiences the same related TEAE ≥Grade 3 twice (excepting exempt TEAEs noted 
in Table 4). 
If a patient meets the individual stopping criteria in the multiple-dose phase, that patient will not 
receive any more doses of WVE-120102.  The patient will be followed up for safety per 
protocol. 


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4.3.5 
Communication of Emergent Safety Concerns 
Enrollment to the study will be closely monitored by the Sponsor.  If the stopping criteria are met 
at any point in the study, enrollment and dosing will be suspended for all cohorts until the data 
are reviewed.  Similarly, if an SAE occurs in the sentinel patients in a cohort, no additional 
patients will be dosed in that cohort until the safety data are reviewed.   
All sites will be informed by fax and email if enrollment and/or dosing is suspended.  In 
addition, if enrollment is suspended, the interactive voice/web response system (IXRS) will be 
adjusted such that no patients can be randomized to treatment or receive additional doses of 
study drug until the safety review has been completed and a decision has been made to resume 
enrollment/dosing.  
5 
PATIENT SELECTION AND WITHDRAWAL CRITERIA 
Approximately 80 patients will be enrolled. Patients will be assigned to study treatment only if 
they satisfy all of the inclusion and none of the exclusion criteria.  If the patient does not have 
documented heterozygosity at SNP2, the patient must undergo Prescreening. 
At Prescreening, patients will be assessed for eligibility for heterozygosity of the targeted SNP, 
CAG triplet repeats (≥36), and presence of the T variant of SNP2 on the same allele as the 
pathogenic CAG expansion.  Patients who do not meet these criteria will be considered 
Prescreen failures.  These results should be recorded for all patients, including those who fail 
Prescreening. 
At Screening, patients who do not meet all remaining inclusion/exclusion criteria will be 
considered screen failures.  The following information should be recorded for all patients who 
fail screening:  demographics, UHDRS Diagnostic Confidence Score, UHDRS TFC score, and 
reason for screen failure.   
5.1 
INCLUSION CRITERIA 
1. Documented ability to understand the written study ICF(s), and has provided signed written 
informed consent prior to any study procedures. 
2. Ambulatory male or female. 
3. Age ≥25 to ≤65 years old. 
4. Body mass index (BMI) ≤32 kg/m2. 
5. Documented CAG triplet repeats ≥36 in the Huntingtin gene. 
6. Documented heterozygosity for SNP2. 
7. Documented presence of the T variant of SNP2 on the same allele as the pathogenic CAG 
expansion 
8. Clinical diagnostic motor features of HD, defined as UHDRS Diagnostic Confidence Score = 
4.  
9. Stage I or Stage II HD, defined as UHDRS TFC scores ≥7 and ≤13. 
10. In the opinion of the Investigator, the patient is able to tolerate all study procedures, and is 
willing to comply with all other protocol requirements. 


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11. Willingness to practice highly effective contraception for the duration of the study if patients 
or their partners are of childbearing potential.  Non-childbearing potential and highly 
effective methods of contraception are defined in the protocol (Section 5.2.1).  
5.2 
EXCLUSION CRITERIA 
1. Malignancy or received treatment for malignancy, other than treated basal cell or squamous 
cell carcinoma of the skin, within the previous 5 years. 
2. Positive for Hepatitis B virus (HBV) or Hepatitis C virus (HCV). 
3. Known to be positive for human immunodeficiency virus (HIV). 
4. Clinically significant medical finding on the physical examination other than HD that, in the 
judgment of the Investigator, will make the patient unsuitable for participation in and/or 
completion of the study procedures. 
5. Received an investigational drug, including an investigational oligonucleotide, within the 
past 1 year or 5 half-lives of the drug, whichever is longer. 
6. Implantable central nervous system (CNS) device that may interfere with ability to 
administer study drug via lumbar puncture or undergo MRI scan. 
7. Diagnostic and Statistical Manual of Mental Disorders 5th Edition (DSM-5) diagnosis at the 
Screening Visit of active alcohol, cannabinoid, or other substance use disorder (except 
nicotine) within 6 months prior to the Screening Visit. 
8. Positive for opioids (unprescribed), cocaine, amphetamines, methadone, barbiturates, 
methamphetamine, or phencyclidine at the Screening Visit.   
9. Started or changed dose for concomitant medication for the treatment of HD symptoms or 
psychiatric disorders within 30 days prior to the Screening Visit (concomitant medications 
that have been administered on a stable regimen for ≥30 days are permitted). 
10. Pregnant (as determined by a serum pregnancy test) or breast feeding at the Screening Visit, 
or plans to become pregnant during the course of the study. 
11. Clinically significant laboratory abnormality at Screening, including, but not limited to: 
a 
Alanine aminotransferase (ALT) or aspartate aminotransferase (AST) values at 
Screening or Baseline >3 times the upper limit of normal (ULN). 
b Renal insufficiency, defined as either serum creatinine >1.8 mg/dL or creatinine 
clearance <40 mL/min. 
12. Clinically significant abnormality at Screening electrocardiogram (ECG), including but not 
necessarily limited to a confirmed QT interval corrected for heart rate (QTc) ≥450 msec for 
males or ≥470 msec for females. 
13. Clinically significant cardiovascular, endocrine, hepatic, renal, pulmonary, gastrointestinal, 
neurologic, malignant, metabolic, psychiatric, or other condition that, in the opinion of the 
Investigator, precludes the patient’s safe participation in the study or would interfere with the 
study assessments.   
14. Bone, spine, bleeding, or other disorder that exposes the patient to risk of injury or 
unsuccessful lumbar puncture. 
15. Inability to undergo brain MRI (with or without sedation).   
16. Deemed to be at significant risk for suicidal behavior based on: 


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a 
The opinion of the Investigator; or 
b Answers “yes” to Actual Suicide Attempts or Suicidal Behaviors in the Suicidal 
Behaviors section of the Columbia-Suicide Severity Rating Scale (C-SSRS) with 
reference to a 2-year period prior to the Screening Visit; or 
c 
Answers “yes” on any items in the Suicidal Ideation section of the C-SSRS with 
reference to a 6-month period prior to the Screening Visit; or 
d Answers “yes” on any items in the Suicidal Ideation section of the C-SSRS at the 
Baseline Visit since the last visit (Screening Visit). 
17. Involved directly or indirectly in the conduct and administration of this study as an 
Investigator, sub-investigator, study coordinator, or other study staff member, or the patient 
is a first-degree family member, significant other, or relative residing with one of the above 
persons involved directly or indirectly in the study. 
5.2.1 
Additional Study Restrictions 
As noted in the inclusion criteria, patients or their partners of childbearing potential must 
practice true abstinence or use highly effective methods of contraception.  True abstinence is 
defined as refraining from heterosexual intercourse for the duration of the trial.  Non-
childbearing potential and highly effective methods of contraception are defined in 
Section 5.2.1.1 and Section 5.2.1.2. 
5.2.1.1 
Non-Childbearing Potential 
Non-childbearing potential is defined as a female who meets either of the following criteria:  
• Postmenopausal state defined as no menses for 12 months without an alternative medical 
cause, or 
• Documented hysterectomy, bilateral tubal ligation, or bilateral oophorectomy.  
5.2.1.2 
Highly Effective Methods of Contraception 
Contraception methods that can achieve a failure rate of <1% per year when used consistently 
and correctly are considered highly effective birth control methods. Such methods are defined as 
1 of the following: 
• True abstinence, defined as refraining from heterosexual intercourse for the duration of the 
trial, when in line with the preferred and usual lifestyle of the subject.  
• Vasectomized partner (if that vasectomized partner is the sole sexual partner and has 
received medical assessment of the surgical success of the vasectomy). 
• An intrauterine hormone-releasing system (IUS). 
• Combined (estrogen and progesterone containing) hormonal contraception associated with 
inhibition of ovulation (oral, intravaginal or transdermal combined). 
• Progesterone-only hormonal contraception associated with inhibition of ovulation (oral, 
injectable, or implantable). 
• An intrauterine device (IUD). 


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Note that periodic abstinence (calendar, symptothermal, post-ovulation methods), 
withdrawal (coitus interruptus), spermicides only, and lactational amenorrhea are not 
acceptable methods of contraception.  
5.3 
Withdrawal Criteria 
5.3.1 
Reasons for Withdrawal/Discontinuation 
The duration of the study is defined for each patient as the date a signed written informed 
consent is provided through the last scheduled follow-up visit or early termination.  Patients may 
withdraw from the study at any time and for any reason without prejudice to their future medical 
care by the Investigator or at the study site. The reasons for patients not completing the study 
will be recorded. A patient may be withdrawn from the study for any of the following reasons: 
• Serious or intolerable AE that in the Investigator’s opinion requires discontinuation of 
treatment.  Patient will be encouraged to remain in the study for safety follow-up.  
• A change in the patient’s medical condition not consistent with the protocol requirements or 
that justifies withdrawal. 
• Lost to follow-up. 
• Pregnancy (refer to Section 9.5). 
• The patient withdraws consent or the Investigator or Sponsor decides to withdraw the from 
the study. 
• Termination of the study by the Sponsor.  
Upon occurrence of a serious or intolerable AE, the Investigator will confer with the Sponsor. If 
a patient is discontinued from the study drug because of an AE, the event will be followed until 
resolution or until the Investigator and the Sponsor agree that further follow-up is not required. 
5.3.2 Handling of Withdrawals 
Patients are free to withdraw from the study or study treatment at any time upon request. Patient 
participation in the study may be stopped at any time at the discretion of the Investigator or at the 
request of the Sponsor, as described in Section 5.3.1. 
Patients who discontinue study treatment or active participation in the study will no longer 
receive study drug but are encouraged to remain in the study for safety follow-up.  When a 
patient withdraws from the study, the reason(s) for withdrawal will be recorded by the 
Investigator on the relevant page of the electronic case report form (eCRF). Whenever possible, 
all patients who discontinue study treatment, or withdraw from the study prematurely, will 
undergo an early termination visit (Table 1, Table 2, and Table 3).  Patients who fail to return for 
final assessments will be contacted by the site in an attempt to have them comply with the 
protocol.  At a minimum, 3 documented telephone calls should be made on different days over 
the course of 2 weeks.  If the patient is unreachable by telephone, a registered letter will be sent 
to the patient requesting him or her to contact the study center.  As part of the informed consent, 
patients will be asked if they are willing to be contacted at the follow-up time point if they have 
discontinued from study drug or withdrawn from the study.  


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It is vital to obtain follow-up data on any patient who discontinues study drug or withdraws 
because of an AE or SAE.  In every case, efforts must be made to undertake safety and follow-up 
procedures as specified by the protocol.  All patients who withdraw from the study with an 
ongoing AE or SAE must be followed until resolution or until the Investigator and the Sponsor 
agree that further follow-up is not required. 
All patients who withdraw from the study due to pregnancy must be followed to record the 
outcome of the pregnancy.  
5.3.3 Replacements 
Patients who drop out before Day 28 (2, 4, 8, and 16 mg cohorts) or Day 14 (32 mg cohort) in 
the single-dose phase will be replaced in accordance with the requirements for dose escalation.  
Patients who drop out after this point may be replaced with patients directly entering the 
multidose part of the study.   
6 
STUDY TREATMENTS 
6.1 
METHOD OF ASSIGNING PATIENTS TO STUDY DRUG 
Patients who meet all of the inclusion criteria and none of exclusion criteria will be randomized 
into the study on Day 1 to receive either active study drug or placebo, both administered 
intrathecally.  Patients will receive WVE-120102 (9 patients in each cohort) or placebo 
(3 patients in each cohort).  
This is a global protocol with some regions only participating in the single-dose component of 
the study.  In order to allow for evaluation of a full multidose cohort (N=12), new patients may 
be enrolled directly in the multiple-dose portion to account for those patients who do not roll 
over from the single-dose portion to the multi-dose portion.  Treatment assignment will be 
performed such that these treatment-naïve patients will receive the same treatment as the patient 
who completed the single-dose portion.  
6.2 
DOSE AND STUDY DRUG ADMINISTRATION 
6.2.1 
Identity of Study Drug 
6.2.1.1 
WVE-120102 
The WVE-120102 will be provided as a lyophilized powder in a clear glass vial for 
reconstitution for solution for injection.  Directions on reconstitution are provided in the 
Pharmacy Manual. 
Laboratory Code: WVE-120102  
Chemistry:  WVE-120102 drug substance is the fully neutralized sodium salt of a mixed 2′-O-
methyl ribonucleic acid/deoxyribonucleic acid oligonucleotide 20-mer containing a prescribed 
combination of 3′-O to 5′-O linked phosphodiester and phosphorothioate linkages, the latter of 
which consist of a prescribed combination of stereodefined Rp and Sp linkages.   
International Nonproprietary Name (INN): None 


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Molecular Formula: The empirical formula is: C206H262N85O112P19S13 
Molecular weight: 6726.13 g/mol (Average) 
6.2.1.2 
Placebo 
Placebo will be 0.9% Sodium Chloride Injection sterile, preservative-free solution administered 
alone via the IT route.  It will be provided in commercially available single-use vials or plastic 
ampoules.  Placebo, as prepared by an unblinded study pharmacist in a dosing solution for 
injection, will be visually identical in appearance to the WVE-120102 injection solution.  
6.2.2 
Administration of Study Drug and Collection of CSF 
Study drug (WVE-120102 or placebo) will be administered as a single dose followed by multiple 
doses (3 doses at 4-week intervals).  The route of administration will be IT by direct lumbar 
injection, and the total volume of the injection will be 10 mL.  The lumbar puncture will be 
performed by an appropriately trained individual.  Within 1 week prior to the lumbar puncture, a 
blood sample will be tested locally for platelet count and prothrombin time to confirm that it is 
safe to proceed with the lumbar puncture (Section 8.2.6).  Patients should not be sedated during 
administration of study drug.  Detailed instructions on the procedure to be used for lumbar 
puncture are provided in the Study Operations Manual. 
Within ≤15 minutes prior to administration of study drug, CSF samples will be collected for 
safety assessments (Section 8.2.7), exposure (Section 8.3), and PD (Section 8.4), per the 
Schedule of Assessments (Table 1 and Table 2).  One extra tube of CSF will be collected and 
stored.  No more than 10 mL of CSF total should be collected. 
Immediately after each administration of study drug, all patients should be ambulatory and active 
for approximately 30 minutes postdose.  The Investigator should note any subtle weakness or 
fatigue during this period.  In addition, formal physical examinations targeting the neurological 
system, will be performed at the time points noted in the Schedule of Assessments (Table 1 and 
Table 2).  Any postdose SAEs will be monitored until resolution.  
Cerebrospinal fluid samples will only be used by the Sponsor and/or a contracted vendor for 
research related to the development of treatments for HD and will be stored for a maximum of 
10 years.  All biological material will be stored and secured in a way that ensures that 
unauthorized access is prohibited and the samples are not lost, deteriorated, or destroyed 
accidentally or illegally.  Detailed instructions for sample collection, storage, processing, and 
shipping will be provided in the Study Operations Manual.   
6.3 
TREATMENT COMPLIANCE 
Provided that a patient attends the clinic visits, treatment non-compliance is not expected to be 
an issue.  Thus, every attempt will be made to ensure regular visits by the patient to the clinic per 
the study schedule.  Investigative staff will make every effort to contact patients who miss visits 
in order to obtain as much follow-up information as possible. 


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6.4 
MANAGEMENT OF CLINICAL SUPPLIES 
6.4.1 
Study Drug Packaging and Storage 
WVE-120102 will be supplied by the Sponsor as a lyophilized powder in clear glass vials.  
Sterile saline (placebo) will be supplied in commercially available single-use vials or plastic 
ampoules.  
All study drugs will be transported, received, stored, and handled in accordance with the 
container or product label, the instructions supplied to the pharmacy, relevant institution’s 
Standard Operating Procedures (SOPs), and applicable regulations. Appropriate storage and 
transportation conditions will be maintained for the study drug from the point of manufacture up 
to delivery of the study drug.  
Study drugs will be stored at 2ºC to 8ºC in a locked area accessible only to the pharmacy 
personnel until reconstitution.  WVE-120102 reconstituted and diluted solution for injection 
contains no preservatives and should be administered without delay or within 4 hours of 
reconstitution.   
Partially used, unused, or damaged vials should be disposed according to Sponsor instructions. 
Details on reconstitution and administration are provided in the Pharmacy Manual and 
Investigator’s Brochure (IB).   
6.4.2 
Study Drug Accountability 
The Investigator will maintain accurate records of receipt of drug supplies, including dates of 
receipt.  In addition, accurate records will be kept regarding when each treatment is 
administered, which patients received treatment, and the name of the personnel administering the 
treatment.  Reasons for departure from the expected treatment regimen must also be recorded.  
Only trained site staff are permitted to treat study patients.  A study monitor will review the 
accountability records onsite.  
6.5 
OTHER SUPPLIES 
The study sites will be provided with the IB, Study Operations Manual, Pharmacy Manual, 
Imaging Manual, Laboratory Manual, laboratory kits, and other materials, as appropriate. 
7 
BLINDING 
7.1 
BLINDING AND RANDOMIZATION 
This is a double-blind and placebo-controlled randomized study. The treatment blind will not be 
broken until the end of the study, except in the case of emergency (Section 7.2). The Sponsor and 
contract research organization (CRO) staff directly responsible for the conduct of data collection 
for the study, the Investigator, and study center staff will be blinded to treatment for the duration 
of the study until such time that all discrepancies in the clinical database are resolved (ie, at the 
time of the database lock).  


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The randomization scheme will be assigned by an IXRS, which will send notifications of the 
treatment assignment to the designated pharmacy personnel.  
The pharmacy personnel will be unblinded to prepare doses of WVE-120102 or placebo to 
ensure that the clinical site staff responsible for administering study drug and conducting 
assessments per the protocol, and the patient, remain blinded to study treatment.  In addition, a 
clinical research associate will be unblinded to perform drug vial accountability and oversee the 
shipping and inventory of study drug.  
7.2 
BREAKING THE BLIND 
A patient’s treatment assignment will not be broken until the end of the study except in the event 
that medical treatment of the patient depends on knowing the study treatment the patient 
received.  If the Investigator feels they need to break the blind in the case of medical emergency, 
they can do so via the IXRS system.     
In addition, certain Sponsor or CRO staff members may be unblinded to the applicable patient’s 
treatment code as required for regulatory reporting of SUSARs. 
Reasons for treatment unblinding must be explained clearly and justified in the eCRF. The date 
on which the code was broken, together with the identity of the person responsible for breaking 
the blind, must also be documented in the patient’s source documents. The unblinded treatment 
information will not be disclosed to anyone who does not need to know the information.  In 
consultation with the medical monitor and the Sponsor, the patient may be withdrawn from the 
study if the blind is broken.   
8 
METHODS OF ASSESSMENT AND ENDPOINTS 
8.1 PRESCREENING ASSESSMENTS 
WVE-120102 is specifically designed to treat HD patients who carry the U isoform of the 
intended SNP on the same allele as the pathogenic CAG expansion (≥36 repeats).  If 
documentation of heterozygosity at SNP2 is not available, patients must have a blood sample 
taken at a Prescreening Visit to enable patient eligibility assessment based on these criteria using 
3 separate methods:   
The number of CAG repeats will be confirmed via polymerase chain reaction (PCR).  
Subsequently, Sanger sequencing will be performed to determine heterozygosity at the targeted 
SNP.   
Only samples for which heterozygosity and required CAG repeats have been confirmed will 
undergo further testing using a long-read sequence analysis to determine whether the T isoform 
of SNP2 is present on the same allele as the pathogenic CAG expansion.  Long-read sequencing 
technology will be performed by a central laboratory using a CE-marked genetic sequencer 
(Pacific Biosciences RS II System).    
Patients confirmed to carry the T isoform of SNP2 on the same allele as the pathogenic CAG 
expansion will be qualified to undergo further screening for confirmation of eligibility.  


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Investigators and patients will be advised only as to whether the patient meets the prescreening 
criteria and will not be provided with specific genetic test results (e.g., number of CAG repeats). 
All blood samples will be sent to central laboratories for processing, analysis, and verification.  
All biological material will be stored and secured in a way that ensures that unauthorized access 
is prohibited and the samples are not lost, deteriorated, or destroyed accidentally or illegally.   
Detailed instructions for sample collection, storage, processing, and shipping will be provided in 
the Study Operations Manual.   
8.2 SAFETY ASSESSMENTS 
The safety assessments will include the following: 
• Adverse events (Section 9) 
• Medical history and demographics 
• Prior and concomitant medications 
• Physical examinations (including neurological and psychiatric) 
• Vital signs 
• 12-lead ECGs 
• Clinical laboratory evaluations (including clinical chemistry, hematology, and urinalysis) 
• Magnetic resonance imaging (Section 8.6) 
• Cerebrospinal fluid sample evaluations (cell count and total protein) 
• Suicidality assessment 
• Pregnancy testing 
Any abnormal laboratory test results (hematology, clinical chemistry, or urine) or other safety 
assessments (e.g., vital sign measurements, C-SSRS), including those that worsen from 
Screening (Visit 2), that are felt to be clinically significant in the medical and scientific judgment 
of the Investigator are to be recorded as AEs or SAEs. 
8.2.1 
Medical History and Demographics 
A general medical history will be obtained at the Screening Visit.  Investigator assessment of 
past medical history at Screening will include information regarding any significant medical, 
surgical, psychiatric, and/or neurological conditions and treatments.  
Whether the patient is diagnosed with Stage 1 or Stage 2 HD, treatments received, and other 
details about this condition will be recorded.   
Demographic data will include date of birth, sex, ethnic categorization, and race. 


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8.2.2 
Prior and Concomitant Medications 
Use of all concomitant medications from 6 weeks before the Screening Visit to the end of the 
patient’s participation in the study will be recorded in the patient’s eCRF.  The minimum 
requirement is that the drug name, dose, indication, and the dates of administration are to be 
recorded.  This will include all prescription drugs, herbal products, vitamins, minerals, and over-
the-counter medications.  Any changes in concomitant medications will also be recorded in the 
patient’s eCRF. 
Any concomitant medication deemed necessary for the welfare of the patient during the study 
may be given at the discretion of the Investigator. However, it is the responsibility of the 
Investigator to ensure that details regarding the medication are recorded in full in the eCRF.  
8.2.3 
Physical Examination 
A physical examination will be performed at the time points noted in the Schedule of 
Assessments (Table 1, Table 2, and Table 3).   
At Screening, the full physical examination will include (but is not limited to) an examination of 
skin; head, eyes, ears, nose, throat; respiratory; cardiovascular; gastrointestinal; endocrine, 
metabolic; blood, lymphatic; musculoskeletal, psychiatric, and neurologic (including mental 
status, cranial nerves, motor system, reflexes, coordination and gait, and sensory system) 
systems.   
Through 4 hours postdose (and up to 24/48 hours postdose following administration on Day 1), a 
targeted physical exam to assess potential motor effects will be performed.  This exam will 
include a neurologic examination with special attention to the motor system, upper and lower 
extremity measures of strength, tone, reflexes, and ambulation.   
At all other time points, the physical exam must include (at a minimum) head, eyes, ears, nose, 
throat, respiratory, cardiovascular, gastrointestinal, musculoskeletal, psychiatric, and neurologic).  
Other systems should be evaluated as appropriate.  Physical findings will be recorded in the 
eCRF and source documents.  
8.2.4 Vital Signs 
Blood pressure (systolic and diastolic), temperature, and pulse will be measured by medically 
qualified personnel at the time points described in the Schedule of Assessments (Table 1, Table 
2, and Table 3), and recorded in the eCRF and source documents. Vital signs measurements will 
be taken as per standard site practice, after the patient has been resting quietly (either lying flat or 
sitting, whichever is most appropriate for the condition of the patient) for a period of at least 
3 minutes. As feasible, the same position (either sitting or lying) should be used for all 
subsequent vital sign measurements during the study for an individual patient. If the initial 
reading is high, the measurements will be repeated twice and the average of the 3 readings will 
be used. 
The patient’s height and weight will be measured at the time points described in the Schedule of 
Assessments (Table 1, Table 2, and Table 3), and recorded in the eCRF and source documents. 


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8.2.5 12-Lead ECG 
Computerized, good quality, 12-lead ECGs will be recorded in triplicate at the time points 
described in the Schedule of Assessments (Table 1, Table 2, and Table 3).  Recordings will be 
obtained in the supine position after the patient has rested comfortably for ≥5 minutes.  
The ECG tracing will be submitted and read by a centralized reviewer (details will be provided 
in the Study Operations Manual).  The following should be recorded on the trace and eCRF: 
whether the ECG is normal or abnormal and, if deemed abnormal, whether the abnormality is 
clinically significant or not clinically significant and note the abnormality.  
8.2.6 Clinical Laboratory Evaluations 
Clinical laboratory safety testing will be collected at the time points described in the Schedule of 
Assessments (Table 1, Table 2, and Table 3) and recorded in the eCRF and source documents.  A 
blood sample will be tested locally within 1 week prior to the lumbar puncture to determine 
platelet count and prothrombin time, and thereby confirm that it is safe to proceed with the 
lumbar puncture.  Other safety laboratory samples will be analyzed at a central laboratory.  Local 
testing on these samples may be conducted as clinically indicated.  
The parameters to be assessed are presented in Table 5.  
Table 5 
Clinical Laboratory Parameters 
Hematology 
Clinical chemistry 
Urine dipstick 
Complete blood count, including: 
• 
White blood cell count (with 
differential) 
• 
Red blood cell count  
• 
Hemoglobin 
• 
Hematocrit 
• 
Platelet count 
• 
Reticulocyte count 
• 
Mean corpuscular volume 
• 
Mean corpuscular hemoglobin 
• 
Mean corpuscular hemoglobin 
concentration 
Activated partial thromboplastin time  
Prothrombin time  
C-reactive protein  
Fibrinogen 
Complement  
Sodium 
Potassium  
Chloride 
Bicarbonate 
Blood urea nitrogen  
Creatinine 
Creatine phosphokinase 
Alanine aminotransferase 
Aspartate aminotransferase 
Alkaline phosphatase 
Bilirubin 
Urinalysis  
 
8.2.7 Cerebrospinal Fluid Safety Lab 
A CSF safety laboratory sample obtained at the times noted on the Schedule of Assessments 
(Table 1, Table 2, and Table 3) will be evaluated at a local lab.  The following parameters will be 
assessed: total protein, glucose, and cell counts (white blood cell counts with differential).     


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Samples will only be used by the Sponsor and/or a contracted vendor for research related to the 
development of treatments for HD and stored for a maximum of 10 years.  All biological 
material will be stored and secured in a way that ensures that unauthorized access is prohibited 
and the samples are not lost, deteriorated, or destroyed accidentally or illegally. Detailed 
instructions for sample collection, storage, processing, and shipping will be provided in the 
Study Operations Manual. 
8.2.8 Suicidality Assessment (Columbia-Suicide Severity Rating Scale) 
The C-SSRS is a measure of suicidal ideations and suicidal behaviors26. The C-SSRS, provided 
in Appendix 2, consists of 2 forms:  a form measuring symptoms at Screening 
(baseline/screening version) that includes a lifetime history, and a form measuring symptoms 
since the last study visit (‘since last visit’ version).  The baseline/screening form will be 
performed at the Screening visit, and the ‘since last visit’ form will be performed at all later time 
points, as described in the Schedule of Assessments (Table 1, Table 2, and Table 3). A trained 
rater will complete this scale.  The findings should be confirmed by the clinical opinion of the 
Investigator. 
The Investigator should be notified if a patient responds “yes” to any of the questions.  The 
Investigator will provide care according to local standards, which may include referral to 
specialists, medical treatment, or hospitalization as necessary. 
8.2.9 Pregnancy Testing  
For female patients of childbearing potential, a negative serum pregnancy test must be 
documented at the Screening Visit and a negative urine pregnancy test must be documented at 
the times noted on the Schedule of Assessments (Table 1, Table 2, and Table 3).  The serum 
pregnancy test will be determined by a central lab; urine pregnancy tests will be performed 
locally.  
8.2.10 Drug Screening 
A urine drug screen for opioids, cocaine, amphetamines, methadone, barbiturates, 
methamphetamine, and phencyclidine will be performed at the times noted on the Schedule of 
Assessments (Table 1, Table 2, and Table 3).  Drug screen tests will be evaluated by a central 
laboratory. 
8.3 
PHARMACOKINETIC ASSESSMENTS  
Cerebrospinal fluid and plasma samples for analysis of exposure toWVE-120102 will be 
collected at the time points specified on Schedule of Assessments (Table 1, Table 2, and Table 
3).  The date and time of the sample collection will be recorded.   
Samples will be analyzed by a central laboratory to determine concentrations of WVE-120102 
using a validated method.   
Samples will only be used by the Sponsor and/or a contracted vendor for research related to the 
development of treatments for HD and stored for a maximum of 10 years.  All biological 
material will be stored and secured in a way that ensures that unauthorized access is prohibited 


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and the samples are not lost, deteriorated, or destroyed accidentally or illegally.   Detailed 
instructions for sample collection, storage, processing, and shipping will be provided in the 
Study Operations Manual.   
8.4 
PHARMACODYNAMIC ASSESSMENTS 
A CSF sample will be collected at the time points noted in the Schedule of Assessments (Table 
1, Table 2, and Table 3) to determine the change from baseline in concentration of mHTT 
protein.  In addition, exploratory biomarkers (e.g., neurofilament light and total tau) may be 
assessed.   
The study may utilize the method described in Wild et al1 or an alternative method to determine 
levels of mHTT protein.  Following unblinding, the study randomization will be provided to the 
bioanalytical laboratory to enable analysis of the PD samples. 
Samples will only be used by the Sponsor and/or a contracted vendor for research related to the 
development of treatments for HD and stored for a maximum of 10 years.  All biological 
material will be stored and secured in a way that ensures that unauthorized access is prohibited 
and the samples are not lost, deteriorated, or destroyed accidentally or illegally.  Detailed 
instructions for sample collection, storage, processing, and shipping will be provided in the 
Study Operations Manual.   
8.5 Immunogenicity Assessments 
Serum samples will be collected at the times noted on the Schedule of Assessments (Table 1, 
Table 2, and Table 3) and analyzed at a central laboratory for measurement of anti-drug 
antibodies to WVE-120102.    
All biological material will be stored and secured in a way that ensures that unauthorized access 
is prohibited and the samples are not lost, deteriorated, or destroyed accidentally or illegally.  
Detailed instructions for sample collection, storage, processing, and shipping will be provided in 
the Study Operations Manual.   
8.6 
MAGNETIC RESONANCE IMAGING  
A 3 Tesla (3T) MRI of the whole brain (without contrast) will be performed at the time points 
noted on the Schedule of Assessments (Table 1, Table 2, and Table 3).  Sedation is permitted 
during the MRI.   
The MRI will be assessed for safety purposes, in addition to exploratory structural assessments.  
Exploratory assessments will include, but are not limited to, volumetric assessments.  Changes in 
MRIs of the brain will be characterized in patients receiving WVE-120102. 
The MRI will be performed by an appropriately trained individual.  Detailed instructions on how 
the MRI will be performed and transferred to the central reader are provided in the Imaging 
Manual.  The scans will be evaluated by a central reader. 


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8.7 ASSESSMENT OF CLINICAL EFFECTS 
8.7.1 Unified Huntington’s Disease Rating Scale  
The UHDRS is a research tool developed by the Huntington Study Group to provide a uniform 
assessment of the clinical features and course of HD27.  The scale consists of 6 subtests, 
including motor assessment, cognitive assessment, behavioral assessment, an independence 
scale, functional assessment, and TFC, and is provided in Appendix 1.  All subtests of the 
UHDRS should be performed except the behavioral assessment, as the PBA-s (Section 8.7.2) 
collects similar behavioral information.  
The motor assessment evaluates motor features of HD with standardized ratings of oculomotor 
function, dysarthria, chorea, dystonia, gait, and postural stability.  The total motor impairment 
score is the sum of all the individual motor ratings, with higher scores indicating more severe 
motor impairment than lower scores. 
The cognitive assessment consists of a lexical verbal fluency test, Symbol Digit Modalities Test 
(SDMT), and the Stroop Interference test.  The Stroop Test results are reported as the raw 
number of correct answers given in a 45-second period.  Results for the other tests are reported 
as the raw number of correct responses. Higher scores indicate better cognitive performance. 
The independence scale is used to follow disease progression in functional disability. The scale 
is rated from 100 (no special care needed) to 0 (tube-fed, total bed care).   
The functional assessment checklist is a 25-question assessment that screens for capacity to 
complete the tasks mentioned in the assessment alone.  The questions are asked in the presence 
of a family or friend to get the clinician’s best judgment based on both responses.  A response of 
“yes” is given a score of 1.  A high score indicates better functioning.  
The TFC is a brief interview involving the patient and a close family member or friend familiar 
with the patient’s functioning.  The measure has 5 items and addresses basic activities of living: 
occupation, handling finances, domestic responsibilities, activities of daily living (e.g., eating, 
dressing, bathing), and level of care.   
The change from baseline to the last measured time point will be determined. 
8.7.2 Short Problem Behaviors Assessment 
The PBA-s is a shorter version of the Problem Behaviors Assessment for HD (PBA-HD), a semi-
structured interview designed to elicit information about behavioral symptoms relevant to HD.  
The shorter version was developed by the Behavioral Phenotype Working Group of the 
European Huntington’s Disease Network (EHDN)28.  
The PBA-s contains 11 items, each measuring a different behavioral problem that is rated for 
both severity and frequency on a 5-point scale.  Severity and frequency ratings are multiplied to 
provide an overall score for each symptom.  The PBA-s is provided in Appendix 3. 


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Interviews are conducted with the patient.  The final rating is determined by assessing all 
available information, including the interviewer’s own observations of the patient’s behavior.  A 
caregiver should not be interviewed as part of the PBA-s for this study.  
The change from baseline to the last measured time point will be determined. 
9 
ADVERSE EVENTS 
The Investigator is responsible for reporting all AEs that are observed or reported during the 
study, regardless of their relationship to study treatment or their clinical significance. 
Adverse events will be assessed beginning at enrollment (date of signed informed consent) and 
up to the end of the study.   
An AE is defined as any untoward medical occurrence in a patient enrolled into this study 
regardless of its causal relationship to study treatment. Patients will be instructed to contact the 
Investigator at any time after randomization if any symptoms develop. 
A TEAE is defined as any event not present before exposure to study treatment or any event 
already present that worsens in either intensity or frequency after exposure to study treatment 
(excluding worsening of results on the UHDRS or PBA-s rating scales).  
9.1 
ELICITING AND DOCUMENTING ADVERSE EVENTS 
All AEs reported or observed during the study, including AEs resulting from concurrent 
illnesses, reactions to concurrent medications, or progression of disease states, will be recorded 
on the AE page in the eCRF and in the site source notes.  The eCRFs used to document AEs are 
designed to help ensure this information is collected in a standard manner.  Information to be 
collected includes event term, date and time of onset, date and time of resolution, 
Investigator-specified assessment of severity and relationship to study treatment, action taken 
with respect to study treatment, seriousness, any required treatment or evaluations, and outcome.  
All AEs will be followed to adequate resolution. The sites will be provided completion 
guidelines for the eCRF, which will further guide them on how to record the data, including AEs.  
The MedDRA will be used to code all AEs.  
Any medical condition that is present at the time that the patient is screened but does not worsen 
should not be reported as an AE.  However, if it worsens at any time during the study, this should 
be recorded as an AE.  This includes any spontaneously reported worsening of depression (ie, 
not based on the study rating scales). 
In addition to observations of the patient, AEs identified from any study data (e.g., laboratory 
values, physical examination findings, ECG changes, C-SSRS) or identified from review of other 
documents that are considered clinically significant will be documented on the AE page in the 
eCRF.  The eCRFs also include a form specifically for a detailed neurological assessment, with 
instructions to report any worsening as an AE.  Worsening of symptoms that are only detected on 
clinical effects rating scales will not be reported as AEs.   


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Adverse events (AEs) will be solicited at each visit by direct questioning as well as elicited from 
physical and neurological examination by site staff.  In addition, all sites in the study must ensure 
patients have a 24-hour telephone number to contact medical site staff for the duration of the 
study, in case of emergent AEs or SAEs. 
If there is any doubt as to whether a clinical observation is an AE, the event should be reported.  
Adverse events characterized as intermittent do not require documentation of onset and duration 
of each episode. 
Pregnancy is not considered an AE.  However, if a patient becomes pregnant during the course of 
the study, the pregnancy must be reported and monitored as described in Section 9.5.  
9.2 
DEFINITIONS OF ADVERSE EVENT SEVERITY AND RELATIONSHIP 
TO STUDY DRUG 
9.2.1 
Severity 
The severity, or intensity, of an AE refers to the extent to which an AE affects the patient’s daily 
activities. Adverse event severity will be evaluated using the NCI CTCAE version 4.0, published 
28 May 2009.  For AEs not included in the NCI CTCAE, the Investigator will be required to 
assess the intensity of the adverse drug/biologic experience using the criteria in Table 6. 
Table 6 
Definitions of AE Severity Using NCI CTCAE Criteria 
Grade 
AE Severity 
Definition  
1 
Mild 
Mild; asymptomatic or mild symptoms; clinical or diagnostic observations only; 
intervention not indicated. 
2 
Moderate 
Moderate; minimal, local or noninvasive intervention indicated. 
3 
Severe 
Severe or medically significant but not immediately life-threatening; hospitalization or 
prolongation of hospitalization indicated; disabling; limiting self care. 
4 
Life 
threatening 
Life-threatening consequences; urgent intervention indicated. 
5 
Death 
Death related to AE. 
Abbreviations: AE = adverse event; CTCAE = Common Terminology Criteria for Adverse Events; NCI = National 
Cancer Institute. 
 
Changes in the severity of an AE should be documented in the eCRF to allow an assessment to 
be performed of the duration of the event at each level of intensity.   
9.2.2 
Relationship to Study Drug 
The Investigator’s assessment of an AE’s relationship to study treatment is part of the 
documentation process, but it is not a factor in determining what is or is not reported in the study.   
The relationship or association of the study drug in causing or contributing to the AE will be 
characterized using the following classification and criteria presented in Table 7. 


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Table 7 
Guidelines for Determining the Relationship (if any) Between Adverse Event 
and the Study Drug 
AE Relationship 
Definition  
Definite 
This relationship suggests that a definite causal relationship exists between treatment 
administration and the AE, and that other conditions (concurrent illness, 
progression/expression of disease state, or concurrent medication reaction) do not 
appear to explain the event. The event reappears or worsens if the study treatment is re-
administered. 
Probable 
This relationship suggests that a reasonable temporal sequence of the event with 
treatment administration exists and, based upon the known pharmacological action of 
the treatment, known or previously reported adverse reactions to the treatment or class 
of treatment, or judgment based on the Investigator’s clinical experience, the 
association of the event with the study treatment seems likely. The event disappears or 
decreases on cessation of study treatment. 
Possible 
This relationship suggests that the study treatment caused or contributed to the AE, ie, 
the event follows a reasonable temporal sequence from the time of treatment 
administration or follows a known response pattern to the study treatment, but could 
also have been produced by other factors. 
Unlikely Related  
This relationship suggests an improbable (but not impossible) association between the 
study medication and the reported event. 
Not Related 
This relationship suggests no association between the study treatment and the reported 
event. 
Abbreviations: AE = adverse event. 
 
9.3 
SERIOUS ADVERSE EVENTS  
9.3.1 
Serious Adverse Event Criteria 
An SAE is defined as any event that results in death, is immediately life threatening, requires 
inpatient hospitalization or prolongation of existing hospitalization, results in persistent or 
significant disability/incapacity, or is a congenital anomaly/birth defect not present at 
Prescreening.  Important medical events that may not result in death, be life threatening, or 
require hospitalization may be considered SAEs when, based upon appropriate medical 
judgment, they may jeopardize the patient or may require medical or surgical intervention to 
prevent one of the outcomes listed in this definition.  Examples of such medical events include 
allergic bronchospasm requiring intensive treatment in an emergency room or at home, blood 
dyscrasias or convulsions that do not result in inpatient hospitalization, or the development of 
drug dependency or drug abuse. 
Serious AEs must be reported (Section 9.3.3) and will be followed through resolution.  Serious 
AEs that occur after the final follow-up visit need not be reported unless the Investigator 
considers them related to study drug.   
9.3.2 
Serious Adverse Event Follow-up 
Appropriate remedial measures should be taken by the Investigator using his/her best medical 
judgment to treat the SAE.  These measures and the patient’s response to these measures should 


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be recorded.  All SAEs regardless of relationship to study drug will be followed by the 
Investigator until resolution.  Clinical, laboratory, and diagnostic measures should be employed 
by the Investigator as needed to determine the etiology of the event adequately. 
9.3.3 
Serious Adverse Event Reporting 
9.3.3.1 
Reporting Requirements 
Any AE that meets SAE criteria (Section 9.3.1) must be reported to the Sponsor and/or designee 
immediately (ie, within 24 hours) after the time site personnel first learn about the event using 
the SAE Report form provided for the study. Regardless of causality, all SAEs must be reported 
and will be collected and recorded from the time the patient signs the ICF at the Prescreening or 
Screening Visit (as applicable) until completion of the final follow-up visit. All SAEs must also 
be recorded in the patient’s source documentation and on the AE page of the patient’s eCRF. 
The initial report should include at least the following information: 
• Study Number 
• Patient’s identification number 
• Description of the event 
• Date and time of onset of the event 
• Seriousness criteria 
• Causality assessment to study drug 
If follow-up is obtained, or requested by the Sponsor and/or designee, the additional information 
should be emailed on an SAE Report Form to Wave, in a timely manner according to the 
procedures outlined above.  Copies of discharge summaries, consultant reports, autopsy reports, 
and any other relevant documents may also be requested. 
The Investigator will be responsible for reporting all SAEs to the Institutional Review Board 
(IRB) or Ethics Committee (EC).  The Sponsor will be responsible for reporting to the regulatory 
authorities and Central Ethics Committees, as per local requirements. 
9.3.3.2 
SAE Contact Information 


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9.4 
OVERDOSE 
The study drugs are planned to be administered by trained study staff.  Administration will be 
performed in accordance with the IB and instructions in the Pharmacy Manual.  Any incidence of 
overdose should be recorded as an SAE.   
No clinical data are available regarding overdose with WVE-120102.  As with any agent, if 
overdose occurs, general supportive measures and close observation should be instituted.  Misuse 
of the study drug for illegal purposes is not expected in this study as patients have no direct 
access to the study drugs. 
9.5 
PREGNANCIES 
If a female subject becomes pregnant during the study, she must be discontinued from study drug 
but the patient may continue to be followed in the study.  The Medical Monitor should be 
notified by the Investigator and a Pregnancy Notification Form should be completed.  The 
patient should be followed until the outcome of the pregnancy is known. 
Pregnancy in and of itself is not an SAE.  However, complications of the pregnancy should be 
reported to the sponsor within 24 hours of knowledge by the Investigator (e.g., if the mother is 
hospitalized for dehydration) and an SAE form must be completed. 
10 STATISTICAL METHODS 
10.1 GENERAL CONSIDERATIONS 
Statistical analysis will be performed using SAS software Version 9.4 or later. Continuous 
variables will be summarized using the mean, standard deviation (SD), median, minimum value, 
and maximum value. Categorical variables will be summarized using frequency counts and 
percentages. Patient-level data will be presented in data listings.  
Analyses will primarily be descriptive; confidence intervals will be presented where appropriate. 
Details on the statistical analysis will be provided in the Statistical Analysis Plan (SAP). 
10.2 SAMPLE SIZE DETERMINATION 
The sample size was not calculated on the basis of statistical hypothesis testing. However, based 
on the results of Wild et al.1, for each of the active doses, 9 patients per active dose will yield a 
two-sided 90% confidence interval width for mHTT in CSF of approximately ±110 fM. 


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10.3 STUDY PATIENTS 
10.3.1 
Analysis Populations 
The following study populations will be evaluated: 
• Safety population will include all randomly assigned patients who receive at least 1 dose of 
WVE-120102.  
• PD population will include all patients in the safety population who have at least 
1 postbaseline CSF sample.  
• PK population will include all patients in the safety population who have sufficient plasma or 
CSF concentration data, as determined by the pharmacokineticist, for inclusion in the 
descriptive statistical analysis. 
• Per Protocol Population will include all patients in the Safety population who have no major 
protocol deviations. 
The Safety population will be used for the safety analysis, demographics, and other baseline 
patient characteristics; the PD population will be used for the PD analysis; the PK population 
will be used for the PK analysis; and the Per Protocol population will be used for all analyses of 
clinical effects. 
10.3.2 
Patient Disposition 
The number and percentage of patients screened, randomized, and included in the safety, PK, 
PD, and Per Protocol populations, and those who complete the study will be presented by dose 
cohort.  The number and percentage of randomized patients who withdraw prior to completion 
and the primary reason for withdrawal will be summarized by dose cohort.   
Study completion information, will be presented by patient in a data listing.  A by-patient listing 
of patients who are screen failures, and the reason for screen failure, will also be presented.  
10.3.3 
Demographics and Other Baseline Characteristics 
Summary statistics for demographic and other baseline characteristics data will be provided by 
dose cohort for the safety population.  
Medical history will be summarized by dose cohort and overall using system organ class (SOC) 
and preferred term (PT).  Events will be summarized by patient incidence rates; therefore, in any 
tabulation, a patient contributes only once to the count for a given PT.   
Medical history findings including the verbatim term will be presented by patient in a data 
listing.  Demographic and baseline data will be provided by patient in data listings. 
10.3.4 
Protocol Deviations 
Assessment of protocol deviations as ‘minor’ or ‘major’ will be determined blinded to treatment 
and prior to database unblinding.  A protocol deviations log will be finalized before unblinding 
and final database lock.  Protocol deviations will be presented by patient in a data listing. 


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10.3.5 
Prior and Concomitant Medications 
Prior and concomitant medications will be summarized separately by the number and percentage 
of patients per dose cohort for each World Health Organization (WHO) drug class and PT.  
Medications will be coded using the WHO drug dictionary, Anatomical Therapeutic Chemical 
(ATC) classes, and PT.  Patients will be counted only once for a given prior or concomitant 
medication. 
Medications with a start date before the first dose of study drug will be classified as prior 
medications.  Any medication that the patient began taking after the first dose of study drug will 
be classified as concomitant.  Any medication that a patient started before the first dose of study 
drug and continued to take during the study will be classified as both prior and concomitant.  
Any medication that was stopped on the same day as the first dose of study drug will be 
considered a prior medication.  If the stop date of a given medication is missing, then the 
medication will be classified as concomitant.   
Concomitant and prior medications will be presented by patient in data listings. 
10.3.6 
Study Drug Exposure and Compliance 
Exposure to study drug, duration of exposure, and overall treatment compliance will be 
summarized.  Study drug dosing information will be presented by patient in data listings. 
10.4 PHARMACOKINETIC ANALYSES 
The plasma and CSF WVE-120102 concentration data will be summarized for patients in the PK 
population.   
The individual patient plasma concentration-time data will be listed and displayed graphically on 
linear and log scales.  The plasma concentration-time data will be summarized descriptively in 
tabular and graphical formats (linear and log scales).   
For each cohort, the plasma WVE-120102 concentration data will be analyzed by 
noncompartmental PK analysis.  The parameters listed in Table 8 and Table 9 will be 
determined.  Additional PK parameters may be evaluated if deemed appropriate. 
Pharmacokinetic parameters for each dose cohort will be compared across WVE-120102 dose 
levels. 
The CSF concentration data will be summarized in tabular format. 


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Table 8 
Pharmacokinetic Parameters of WVE-120102 after Single-Dose Administration 
Parameter 
Definition 
Cmax 
Maximum observed concentration 
tmax 
Time of occurrence of Cmax 
AUC0-t 
Area under the plasma concentration-time curve from time zero to the last 
quantifiable concentration 
Table 9 
Pharmacokinetic Parameters of WVE-120102 after Multiple Dose 
Administration  
Parameter 
Definition 
Cmax 
Maximum observed concentration  
tmax 
Time of occurrence of Cmax  
λz  
Terminal elimination rate constant  
AUC0-τ  
Area under the plasma concentration-time curve over the dosing interval  
10.5 PHARMACODYNAMIC ANALYSES 
Pharmacodynamic analyses will be performed for all patients in the PD population.  Summary 
statistics will be provided for PD endpoints by dose cohort and visit.  The change from baseline 
(Day 1) to the last measured time point will be determined.  Individual patient data will be listed.  
Additional details on the statistical analysis of PD endpoints will be provided in the SAP.  
10.6 IMMUNOGENCITY ASSESSMENT 
Immunogenicity analyses will be performed for all patients.  Summary statistics will be provided 
as appropriate.  Individual patient data will be listed.  Additional details on the statistical analysis 
of immunogenicity will be provided in the SAP.  
10.7 MAGNETIC RESONANCE IMAGING 
Summary statistics will be provided for MRI data by dose cohort and visit.  Individual patient 
data will be listed.  Additional details on the statistical analysis of MRI data will be provided in 
the SAP.  
10.8 CLINICAL EFFECTS ANALYSES 
Clinical effects analyses will be performed for all patients in the Per Protocol population.  
10.8.1 
Unified Huntington’s Disease Rating Scale 
Summary statistics will be provided for each subtest of the UHDRS (TFC, motor, cognitive, 
independence, and functional assessments) by dose cohort and visit.  A composite score of some 
or all of the components of the UHDRS may also be calculated.  The change from baseline to the 
last measured time point will be determined.  Individual patient data will be listed.  Additional 
details on the statistical analysis of UHDRS data will be provided in the SAP.  


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10.8.2 
Short Problem Behaviors Assessment 
Summary statistics will be provided for PBA-s data by dose cohort and visit.  The change from 
baseline to the last measured time point will be determined.  Individual patient data will be listed.  
Additional details on the statistical analysis of PBA-s data will be provided in the SAP. 
10.9 SAFETY ANALYSES 
Safety data will be analyzed descriptively overall and by treatment group and dose cohort, and 
no formal statistical comparisons will be performed.  Data from unscheduled visits (laboratory 
tests, vital signs, physical examinations, and ECGs) will not be included in the tables, but will be 
included in the by-patient listings. 
10.9.1 Adverse Events 
Adverse events will be collected and recorded from the time the patient signs the ICF at the 
Prescreening Visit to the study completion.  The verbatim AE term will be coded to the PT and 
SOC using terminology from the MedDRA. 
A TEAE is defined as an AE that is first identified, or is identified to worsen in intensity, at a 
time point occurring after the first dose of study drug.  The numbers and percentages of patients 
with any TEAE, with any TEAE assessed by the Investigator as related to study drug or to study 
procedures, and with any treatment-emergent SAE will be summarized by treatment group and 
overall.  Treatment-emergent AEs will be tabulated by relationship to study drug and by severity; 
these summaries will be presented by SOC and PT, by treatment group and overall.  In these 
tabulations, each patient will contribute only once (ie, the most related occurrence or the most 
intense occurrence) to each of the incidence rates in the descriptive analysis, regardless of the 
number of episodes. 
All AEs and SAEs occurring during the study (pretreatment, treatment, and follow-up) will be 
presented by patient in data listings.  By-patient listings will also be provided for deaths, SAEs, 
and AEs leading to study drug discontinuation. 
10.9.2 Clinical Laboratory Evaluations 
Laboratory test values and changes from baseline over time will be summarized for each 
continuous clinical laboratory parameter.  Shift tables showing the pattern of change from 
baseline to postbaseline visit(s) will be presented.  
All laboratory data will be presented by patient in data listings.  For hematology and chemistry 
laboratory data, the laboratory normal ranges will be provided, and individual abnormal 
laboratory values will be flagged and clinical significance will be indicated.  A separate listing of 
only clinically significant hematology and chemistry findings will be provided.  
10.9.3 Vital Signs 
The actual value and change from baseline to each postbaseline assessment will be summarized 
for vital signs (systolic and diastolic blood pressure, pulse rates, and weight).  Height and body 
mass index will also be presented.  Vital signs measurements will be presented by patient in data 
listings. 


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10.9.4 Electrocardiogram 
Summary statistics will be provided for ECG data by dose cohort and visit.  Individual patient data 
will be listed. 
10.9.5 Magnetic Resonance Imaging (Safety) 
Summary statistics will be provided for MRI safety data by dose cohort and visit.  Individual 
patient data will be listed.  Additional details on the statistical analysis of MRI data will be 
provided in the SAP. 
10.9.6 Cerebrospinal Fluid Safety Lab 
Cerebrospinal fluid safety lab values and changes from baseline over time will be summarized 
for each continuous clinical laboratory parameter.  Shift tables showing the pattern of change 
from baseline to each postbaseline visit will be presented.  
All data will be presented by patient in data listings. 
10.9.7 Columbia-Suicide Severity Rating Scale 
Data from the C-SSRS will be descriptively summarized by dose cohort.  By-patient listings of 
the results will be provided. 
10.10 INTERIM ANALYSES 
Administrative analyses including mHTT may be performed for planning purposes. These 
analyses will include unblinded summaries by treatment group. The implementation of these 
analyses is described in a Data Access Plan.   
10.11 DATA QUALITY ASSURANCE 
10.11.1 Electronic Case Report Forms and Data Management 
All data relating to the study will be recorded in the patient’s source documentation and eCRF to 
be provided by the Sponsor or designee via the electronic data capture (EDC) system.  The 
eCRFs are to be completed within 5 working days of the patient’s visit, except for results of tests 
performed outside the Investigator’s office.  Source documentation supporting the eCRF data 
should indicate the patient’s participation in the study and should document the dates and details 
of study procedures, AEs, all observations, and patient status.  The Investigator is responsible for 
verifying that all data entries on the eCRFs are accurate and correct and ensuring that all data are 
entered in a timely manner, as soon as possible after information is collected.  An explanation 
should be provided for all missing data. The Investigator must provide, through EDC, formal 
approval of all the information on the eCRFs and changes to the eCRFs to endorse the final 
submitted data for the patients for whom the Investigator is responsible. 
The Sponsor will retain the final eCRF data and corresponding audit trails.  A copy of the final 
archival eCRF in the form of a compact disc or other electronic media will be placed in the 
Investigator’s study file. 
A record of patient screen failures, including the reason for the failure, will be maintained for 
patients who do not qualify for enrollment. 


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11 REGULATORY, ETHICAL, AND LEGAL OBLIGATIONS 
11.1 DECLARATION OF HELSINKI 
The Sponsor and Investigator(s) will ensure that this study is conducted in accordance with the 
most recent revision of the Declaration of Helsinki. 
11.2 GOOD CLINICAL PRACTICE 
The study will be conducted according to the study protocol and SOPs that meet the guidelines 
provided by the International Conference on Harmonisation (ICH) for Good Clinical Practice 
(GCP) in clinical studies, and any other applicable local regulatory requirements. 
11.3 INSTITUTIONAL REVIEW BOARDS/ETHICS COMMITTEES 
Federal regulations and ICH guidelines require that approval be obtained from an IRB or EC 
before participation of human patients in research studies. Before study onset, the protocol, 
informed consent form, advertisements to be used for the recruitment of study patients, and any 
other written information regarding this study to be provided to the patient or the patient’s legal 
guardian must be approved by the IRB or EC. Documentation of all IRB/EC approvals and of the 
IRB/EC compliance with ICH guideline E6(R2): GCP will be maintained by the site and will be 
available for review by the Sponsor or its designee. 
All IRB/EC approvals should be signed by the chairman or designee and must identify the 
IRB/EC name and address, the clinical protocol by title or protocol number or both, and the date 
approval or a favorable opinion was granted. The study protocol, appendices, and ICFs must be 
approved by the IRB/EC.  
The Investigator is responsible for providing written summaries of the progress and status of the 
study at intervals not exceeding one year or otherwise specified by the IRB/EC. The Investigator 
must promptly supply the Sponsor or its designee, the IRB/EC, and, where applicable, the 
institution, with written reports on any changes significantly affecting the conduct of the study or 
increasing the risk to patients. 
11.4 INFORMED CONSENT FORMS 
Signed ICFs in compliance with the Declaration of Helsinki, current ICH and GCP guidelines, 
US Title 21 CFR Part 50, and applicable local regulations will be obtained from each patient 
before enrolling the patient in the study or performing any unusual or non-routine procedure that 
involves risk to the patient.  Patients may sign 2 separate ICFs.  Some patients may sign an ICF 
to indicate consent specifically for Prescreening procedures.  All patients will sign a study ICF 
prior to Screening and subsequent study procedures. 
Informed consent form templates will be provided by the Sponsor to investigative sites.  If any 
institution-specific modifications to study-related procedures are proposed or made by the site, 
the ICF(s) must be reviewed by the Sponsor or its designee or both before IRB/EC submission.  
Once reviewed, the ICF(s) will be submitted by the Investigator to his or her IRB/EC for review 
and approval before the start of the study.  If the ICF(s) is revised during the course of the study, 
all actively participating patients must sign the revised form. 


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Before Prescreening (as applicable) and Screening, each prospective patient will be given a full 
explanation of prescreening procedures or the study and be allowed to read the approved 
Prescreening/Study ICF.  Once the Investigator is assured that the patient understands the 
implications of participating in the study, the patient will be asked to give consent to participate 
in Prescreening or the study by signing the appropriate ICF. 
The Investigator will retain the signed original ICF(s) and give a copy of the signed original 
form(s) to the patient. 
12 INVESTIGATOR’S OBLIGATIONS 
The following administrative items are meant to guide the Investigator in the conduct of the 
study but may be subject to change based on industry and government SOPs, working practice 
documents, or guidelines. Changes will be reported to the IRB/EC but will not result in protocol 
amendments. 
12.1 CONFIDENTIALITY 
All laboratory specimens, evaluation forms, reports, and other records will be identified in a 
manner designed to maintain patient confidentiality.  All records will be kept in a secure storage 
area with limited access.  Clinical information will not be released without the written 
permission of the patient, except as necessary for monitoring and auditing by the Sponsor, its 
designee, applicable regulatory agencies, or the IRB/EC. 
The Investigator and all employees and coworkers involved with this study may not disclose or 
use for any purpose other than performance of the study any data, record, or other unpublished 
confidential information disclosed to those individuals for the purpose of the study.  Prior written 
agreement from the Sponsor or its designee must be obtained for the disclosure of any said 
confidential information to other parties. 
12.2 INVESTIGATOR DOCUMENTATION 
Prior to beginning the study, the Investigator will be asked to comply with ICH E6(R2) 8.2 and 
Title 21 of the CFR by providing the following essential documents, including but not limited to: 
• IRB/EC approval. 
• A fully executed Clinical Trial Agreement. 
• Original Investigator-signed Investigator agreement page of the protocol. 
• Curriculum vitae for the Investigator and each sub-investigator listed on the IRB/EC 
application. 
• Financial disclosure information, as applicable. 
• IRB/EC-approved informed consent, samples of site advertisements for recruitment for this 
study, and any other written information regarding this study that is to be provided to the 
patient. 
• Laboratory certifications and normal ranges for any local laboratories used by the site. 


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12.3 STUDY CONDUCT 
The Investigator agrees that the study will be conducted according to the principles of 
ICH E6(R2).  The Investigator will conduct all aspects of this study in accordance with all 
national, state, and local laws or regulations. Study information from this protocol will be posted 
on publicly available clinical trial registers before enrollment of patients begins. 
12.4 ADHERENCE TO PROTOCOL 
The Investigator agrees to conduct the study as outlined in this protocol in accordance with ICH 
E6(R2) and all applicable guidelines and regulations. 
12.5 ADVERSE EVENTS AND STUDY REPORT REQUIREMENTS 
By participating in this study, the Investigator agrees to submit reports of SAEs according to the 
timeline and method outlined in the protocol.  In addition, the Investigator agrees to submit 
annual reports to the IRB/EC as appropriate. 
12.6 INVESTIGATOR’S FINAL REPORT 
Where applicable, the Investigator should inform the institution of study completion; the 
investigator/institution should provide the IRB/EC with a summary of the study’s outcome and 
the Sponsor and regulatory authority(ies) with any reports required. 
12.7 RECORDS RETENTION 
The Investigator/institution will retain essential documents until at least 2 years after the last 
approval of a marketing application in an ICH region and until there are no pending or 
contemplated marketing applications in an ICH region or at least 2 years have elapsed since the 
formal discontinuation of clinical development of the study drug.  However, these documents 
will be retained for a longer period if required by the applicable regulatory requirements or by an 
agreement with the Sponsor.  It is the responsibility of the Sponsor to inform the 
investigator/institution as to when these documents no longer need to be retained. 
12.8 PUBLICATIONS 
All information regarding WVE-120102 supplied by the Sponsor to the Investigator or generated 
as a result of any clinical studies is privileged and confidential information belonging to the 
Sponsor.  The Investigator agrees to use Sponsor’s confidential information solely to accomplish 
the study and will not use such information for any other purposes without the prior written 
consent of the Sponsor.  The Investigator is obligated to provide the Sponsor with complete and 
accurate data obtained during the study.  The information obtained from the clinical study will be 
used toward the development of WVE-120102 and may be disclosed by the Sponsor to 
regulatory authority(ies), other investigators, corporate partners, and consultants as required. 
It is anticipated that the results of this study may be presented at scientific meetings and/or 
published in a peer-reviewed scientific or medical journal.  A Publications Committee will be 
formed (Section 13.4) to oversee any publication or presentation of the study results.  
Subsequently, individual Investigators may present or publish results from the study in 
compliance with their agreements with the Sponsor.  A draft abstract/manuscript is to be 


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provided to the Sponsor at least 30 days prior to submission of the abstract/manuscript to a 
medical/scientific organization or a publisher. 
13 STUDY COMMITTEES 
13.1 SAFETY MONITORING COMMITTEE 
An unblinded, independent SMC consisting of at least 3 members (including a statistician and 2 
physicians of whom 1 must be a neurologist) will review unblinded, aggregate safety data 
periodically.  In addition, the SMC will review SAEs that occur in the sentinel patients and 
TEAEs that meet the stopping criteria.   The SMC will also review dose recommendations from 
the Dose Escalation Committee and the chairperson will endorse the decisions.  Details on safety 
reviews are provided in Section 4.3.    
Further details regarding the SMC, including committee membership, will be provided in a SMC 
Charter.   
13.2 DOSE ESCALATION COMMITTEE 
The decision on escalation to each subsequent single dose will be made by the Dose Escalation 
Committee.  In addition, the Dose Escalation Committee will determine if a single-dose cohort 
can proceed to multiple dosing.  The Dose Escalation Committee will review blinded data.  This 
committee will include, but is not limited to, a Sponsor representative, a Medical Monitor, and a 
member of the Clinical Advisory Committee.  Details on safety reviews are provided in 
Section 4.3.  Further details regarding the Dose Escalation Committee, including committee 
membership, will be provided in a Dose Escalation Committee Charter.   
13.3 CLINICAL ADVISORY COMMITTEE 
A Clinical Advisory Committee, consisting of a Study Investigator and experts in Huntington’s 
disease, will be formed to provide advice regarding protocol and study conduct.  Further details 
regarding the Clinical Advisory Committee, including committee membership, will be provided 
in a Clinical Advisory Committee Charter.   
13.4 PUBLICATIONS COMMITTEE 
A Publications Committee, consisting of Investigators participating in the study, at least one 
member of the Clinical Advisory Committee, and representatives from the Sponsor as 
appropriate, will be formed to oversee any publication or presentation of the study results, which 
will reflect the experience of all participating study centers.   
14 STUDY MANAGEMENT 
14.1 MONITORING 
14.1.1 
Monitoring of the Study 
Monitoring and auditing procedures developed by the Sponsor or designee will be followed in 
order to comply with ICH GCP guidelines.  


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Before a study center can enter a patient into the study, a representative of the Sponsor or 
designee will assess the study center to: 
• Determine the adequacy of the facilities. 
• Discuss with the Investigator(s) and other personnel their responsibilities with regard to 
protocol adherence, and the responsibilities of the Sponsor or its representatives. The 
Investigator responsibilities will be documented in a Clinical Trial Agreement between 
the Sponsor and the Investigator. 
Note that if the Sponsor or designee has experience with the study center within the previous 
12 months, an abbreviated qualification assessment may be performed via e-mail and/or 
telephone. 
During the study, a monitor from the Sponsor or designee will have regular contact with the 
study center for the following: 
• Provide information and support to the Investigator(s). 
• Confirm that facilities remain acceptable. 
• Confirm that the investigational team is adhering to the protocol, that data are being 
recorded accurately in the source documents and eCRFs, and that investigational product 
accountability checks are being performed. 
• Perform source data verification. This includes a comparison of the data in the eCRFs 
with the patient’s medical records at the hospital or practice, and other records relevant to 
the study. Verification will require direct access to all original records for each patient 
(e.g., clinic charts). 
• Record and report any protocol deviations not sent to the Sponsor or designee previously. 
• Confirm AEs and SAEs have been documented properly in the eCRFs and confirm any 
SAEs have been forwarded to the Sponsor, and those SAEs that met criteria for reporting 
(ie, serious adverse drug reactions) have been forwarded to the IRB/EC. 
The monitor will be available between visits if the Investigator(s) or other staff needs 
information or advice. 
14.1.2 
Inspection of Records 
Investigators and institutions involved in the study will permit study-related monitoring, audits, 
IRB/EC review, and regulatory inspections by providing direct access to all study records. In the 
event of an audit, the Investigator agrees to allow the Sponsor, representatives of the Sponsor, or 
regulatory authorities access to all study records. 
The Investigator should notify the Sponsor promptly of any audits scheduled by any regulatory 
authorities and will promptly forward copies of any audit reports received to the Sponsor. 


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14.2 MANAGEMENT OF PROTOCOL AMENDMENTS AND DEVIATIONS 
14.2.1 
Modification of the Protocol 
Any changes in this research activity, except those necessary to remove an apparent, immediate 
hazard to the patient, must be reviewed and approved by the Sponsor or designee. Amendments 
to the protocol, other than minor clarifications and typographical corrections, must be submitted 
in writing to the Investigator’s IRB/EC and regulatory authorities for approval before patients 
can be enrolled into an amended protocol. 
14.2.2 
Protocol Deviations 
A deviation from the protocol is an unintended or unanticipated departure from the procedures or 
processes approved by the Sponsor and the IRB/EC and agreed to by the Investigator.   
The Investigator or designee must document and explain in the patient’s source documentation 
any deviation from the approved protocol. The Investigator may implement a deviation from the 
protocol to eliminate an immediate hazard to study patients without prior IRB/EC approval.  As 
soon as possible after such an occurrence, the implemented deviation, the reasons for it, and any 
proposed protocol amendments should be submitted to the IRB/EC for review and approval, to 
the Sponsor for agreement, and to the regulatory authorities, if required. 
Protocol deviations will be documented by the clinical monitor throughout the course of 
monitoring visits. Investigators will be notified in writing by the monitor of deviations.  As 
required by local regulatory authorities, the Investigator will notify the IRB/EC of any applicable 
protocol deviations in a timely manner. 
14.3 STUDY TERMINATION 
Although the Sponsor has every intention of completing the study, the Sponsor reserves the right 
to temporarily suspend or terminate the study at any time for any reason including (but not 
limited to) safety issues, ethical issues, or severe noncompliance. 
The end of the study is defined as the date on which the last patient completes the last visit 
(includes follow-up visit). 
Upon completion or termination of the study, the study monitor will conduct site closure 
activities with the Investigator or site staff (as appropriate), in accordance with applicable 
regulations, ICH GCP, and SOPs. 
14.4 FINAL REPORT 
Whether the study is completed or terminated prematurely, the Sponsor will ensure that a final 
report is prepared and provided to the regulatory agency(ies), as applicable. The Sponsor will 
also ensure that the clinical study reports (CSRs) in marketing applications meet the standards of 
the ICH Guideline E3: Structure and content of clinical study reports (CSRs). 
Where required by applicable regulatory requirements, a Principal Investigator will be identified 
for the approval and signoff of the clinical study report. The Principal Investigator will be 


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provided reasonable access to statistical tables, figures, and relevant reports and will have the 
opportunity to review the complete study results. 
The Investigator is encouraged to share the summary results with the study patients, as 
appropriate. The study results will be posted on publicly available clinical trial registers. 
 


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15 REFERENCES 
1. 
Wild EJ, Boggio R, Langbehn D, et al. Quantification of mutant huntingtin protein in 
cerebrospinal fluid from Huntington's disease patients. J Clin Invest. 2015;125(5):1979-
1986. 
2. 
Bates G, Tabrizi S, Jones L, (editors). Huntington's Disease, 4th Edition. Oxford (UK): 
Oxford University Press; 2014. 
3. 
Sturrock A, Leavitt BR. The clinical and genetic features of Huntington disease. J 
Geriatr Psychiatry Neurol. 2010;23(4):243-259. 
4. 
Pringsheim T, Wiltshire K, Day L, Dykeman J, Steeves T, Jette N. The incidence and 
prevalence of Huntington's disease: a systematic review and meta-analysis. Mov Disord. 
2012;27(9):1083-1091. 
5. 
Liou S. Population genetics and Huntington's disease. Hopes: Huntington's Outreach 
Project for Education, at Stanford 2010; http://web.stanford.edu/group/hopes/cgi-
bin/hopes_test/population-genetics-and-hd/. Accessed 31 August, 2016. 
6. 
National Organization for Rare Disorders (NORD). Huntington's Disease.  
http://rarediseases.org/rare-diseases/huntingtons-disease/. Accessed 27 June, 2016. 
7. 
A novel gene containing a trinucleotide repeat that is expanded and unstable on 
Huntington's disease chromosomes. The Huntington's Disease Collaborative Research 
Group. Cell. 1993;72(6):971-983. 
8. 
Dragatsis I, Levine MS, Zeitlin S. Inactivation of Hdh in the brain and testis results in 
progressive neurodegeneration and sterility in mice. Nat Genet. 2000;26(3):300-306. 
9. 
Leavitt BR, van Raamsdonk JM, Shehadeh J, et al. Wild-type huntingtin protects neurons 
from excitotoxicity. J Neurochem. 2006;96(4):1121-1129. 
10. 
Rigamonti D, Sipione S, Goffredo D, Zuccato C, Fossale E, Cattaneo E. Huntingtin's 
neuroprotective activity occurs via inhibition of procaspase-9 processing. J Biol Chem. 
2001;276(18):14545-14548. 
11. 
Zhang Y, Leavitt BR, van Raamsdonk JM, et al. Huntingtin inhibits caspase-3 activation. 
EMBO J. 2006;25(24):5896-5906. 
12. 
DiFiglia M, Sena-Esteves M, Chase K, et al. Therapeutic silencing of mutant huntingtin 
with siRNA attenuates striatal and cortical neuropathology and behavioral deficits. Proc 
Natl Acad Sci U S A. 2007;104(43):17204-17209. 
13. 
Kordasiewicz HB, Stanek LM, Wancewicz EV, et al. Sustained therapeutic reversal of 
Huntington's disease by transient repression of huntingtin synthesis. Neuron. 
2012;74(6):1031-1044. 
14. 
Kay C, Skotte NH, Southwell AL, Hayden MR. Personalized gene silencing therapeutics 
for Huntington disease. Clin Genet. 2014;86(1):29-36. 
15. 
Lombardi MS, Jaspers L, Spronkmans C, et al. A majority of Huntington's disease 
patients may be treatable by individualized allele-specific RNA interference. Exp Neurol. 
2009;217(2):312-319. 
16. 
Beverly M, Hartsough K, Machemer L. Liquid chromatography/electrospray mass 
spectrometric analysis of metabolites from an inhibitory RNA duplex. Rapid Commun 
Mass Spectrom. 2005;19(12):1675-1682. 
17. 
Geary RS, Baker BF, Crooke ST. Clinical and preclinical pharmacokinetics and 
pharmacodynamics of mipomersen (kynamro((R))): a second-generation antisense 
oligonucleotide inhibitor of apolipoprotein B. Clin Pharmacokinet. 2015;54(2):133-146. 


WVE-120102 
 
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Clinical Study Protocol: WVE-HDSNP2-001 
 
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18. 
Vuillemenot BR, Korte S, Wright TL, Adams EL, Boyd RB, Butt MT. Safety Evaluation 
of CNS Administered Biologics-Study Design, Data Interpretation, and Translation to the 
Clinic. Toxicol Sci. 2016;152(1):3-9. 
19. 
Butt MT. Morphologic changes associated with intrathecal catheters for direct delivery to 
the central nervous system in preclinical studies. Toxicol Pathol. 2011;39(1):213-219. 
20. 
Chen L, Jiang M, Pei L. Comparison of three methods of drug delivery in the rat lumbar 
spinal subarachnoid space. Anat Rec (Hoboken). 2012;295(7):1212-1220. 
21. 
Frazier KS. Antisense oligonucleotide therapies: the promise and the challenges from a 
toxicologic pathologist's perspective. Toxicol Pathol. 2015;43(1):78-89. 
22. 
Henry SP, Novotny W, Leeds J, Auletta C, Kornbrust DJ. Inhibition of coagulation by a 
phosphorothioate oligonucleotide. Antisense Nucleic Acid Drug Dev. 1997;7(5):503-510. 
23. 
Hodgson PS, Neal JM, Pollock JE, Liu SS. The neurotoxicity of drugs given intrathecally 
(spinal). Anesth Analg. 1999;88(4):797-809. 
24. 
Chordas C. Post-dural puncture headache and other complications after lumbar puncture. 
J Pediatr Oncol Nurs. 2001;18(6):244-259. 
25. 
Evans RW, Armon C, Frohman EM, Goodin DS. Assessment: prevention of post-lumbar 
puncture headaches: report of the therapeutics and technology assessment subcommittee 
of the american academy of neurology. Neurology. 2000;55(7):909-914. 
26. 
Posner K, Brown GK, Stanley B, et al. The Columbia-Suicide Severity Rating Scale: 
initial validity and internal consistency findings from three multisite studies with 
adolescents and adults. Am J Psychiatry. 2011;168(12):1266-1277. 
27. 
Huntington Study Group. Unified Huntington's Disease Rating Scale: reliability and 
consistency. Huntington Study Group. Mov Disord. 1996;11(2):136-142. 
28. 
Callaghan J, Stopford C, Arran N, et al. Reliability and factor structure of the Short 
Problem Behaviors Assessment for Huntington's disease (PBA-s) in the TRACK-HD and 
REGISTRY studies. J Neuropsychiatry Clin Neurosci. 2015;27(1):59-64.