Conditions: Healthy Type 2 Diabetes
Keywords: GLP-1 Insulin secretion Macronutrients
Allocation: NON_RANDOMIZED | Intervention model: CROSSOVER
Enrollment: 12
Healthy volunteers: yes

Brief summary:
The regulation by DPP-4 inhibition after ingested of different individual macronutrients is not known. Therefore, this study examines the influence of ingestion of fat, protein, glucose or mixed meal on the concentrations of incretin hormones and insulin secretion with or without concomitant administration of a DPP-4 inhibitor (sitagliptin).

Detailed description:
Meal ingestion releases gut hormones which stimulate insulin secretion. A most important gut hormone is the incretin hormone glucagon-like peptide-1 (GLP-1). GLP-1 is rapidly degraded and inactivated after its release by the enzyme dipeptidyl peptidase-4 (DPP-4). Inhibition of DPP-4 therefore increases the concentration of active GLP-1 after meal ingestion, which augments insulin secretion. How this process is regulated after ingested of different individual macronutrients is not known. Therefore, this study examines the influence of ingestion of fat, protein, glucose or mixed meal on the concentrations of incretin hormones and insulin secretion with or without concomitant administration of a DPP-4 inhibitor (sitagliptin).

Arms:
arm 1: A placebo tablet is given before ingestion of macronutrients arm 2: Sitagliptin is given before ingestion of macronutrients

Interventions: intervention 1: Sitagliptin intervention 2: placebo
Intervention descriptions: intervention 1: 100 mg sitagliptin before ingestion of macronutrient intervention 2: A placebo tablet is given before ingestion of macronutrients

Protocol document: