Conditions: Vascular System Injuries Lipid Metabolism Disorders Endothelial Disfunction
Keywords: sirtuin atherosclerosis longevity calorie restriction resveratrol vascular reactivity
Allocation: RANDOMIZED | Intervention model: PARALLEL
Enrollment: 48
Healthy volunteers: yes

Brief summary:
Women have a natural protection that gives them greater longevity. One hypothesis most commonly used is the estrogen protection in the premenopausal period. However many studies of various forms of hormone replacement therapy proved ineffective in promoting additional protection for women. Thus, it is discussed other ways of protection associated with longevity in women. Of these, the sirtuin system was found in several animal studies to be associated with longevity. This system also showed, through the involvement of several metabolic pathways, an important protection against the process of atherosclerosis. But the activity of this system in humans is unknown and if it is more active in women than in men. The study's main objectives are to analyze this system in healthy 24 women and 24 men aged 55 to 65 years, and their influence on the main metabolic pathways related to longevity and the process of atherosclerosis. The research protocol includes analysis of the influence of sirtuin (SIRT1) in vascular reactivity, lipid profile, antioxidant capacity, markers of inflammation and homeostasis, before and after the interventions with caloric restriction or resveratrol administration. It is expected of this study mechanistic conclusion for longevity and possible clinical applications in the mechanism of atherosclerosis prevention.

Detailed description:
After a washout period of 15 days without medication, men and women will be randomized to the group of calorie restriction or resveratrol so that in 24 individuals (12 women and 12 men) will be given a hypocaloric diet (1000 calories per day) and the remaining 24 individuals (12 women and 12 men), a daily pill of 500mg of resveratrol. In the two moments, after the washout period and before the intervention (baseline) and after 30 days of intervention (final) will evaluate the clinical, laboratory and vascular reactivity of all subjects.

Arms:
arm 1: 12 men will receive a pill with 500mg of resveratrol daily for 30 days arm 2: 12 women will receive a pill with 500mg of resveratrol daily for 30 days arm 3: 12 men will follow a 1000 calories per day diet for 30 days arm 4: 12 women will follow a 1000 calories per day diet for 30 days

Interventions: intervention 1: Resveratrol intervention 2: Caloric restriction
Intervention descriptions: intervention 1: 1 pill daily containing 500 mg/d of resveratrol for 30 days intervention 2: Diet of 1000kcal per day for 30 days

Protocol document: