Conditions: Chronic Periodontitis
Keywords: Chronic periodontitis Minocycline Surface and root planning Pocket depth Sulcus bleeding index Prevotella intermedia
Allocation: RANDOMIZED | Intervention model: PARALLEL
Enrollment: 70
Healthy volunteers: no

Brief summary:
To evaluate the respective or combinatory efficacy of locally delivered 2% Minocycline (MO) and surface and root planning (SRP) by assessing both clinical parameters and the loads of four main periodontal pathogens in treating chronic periodontitis.

Detailed description:
In this randomized clinical trial, the investigators will evaluate the respective or combinatory efficacy of minocycline and scaling and root planning in the aspects of both clinical parameters \[Pocket depth (PD) and sulcus bleeding index (SBI)\] and the loads of four main periodontal pathogens \[Aggregatibacter actinomycetemcomitans (Aa), Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg) and Prevotella intermedia (Pi)\]. Real-time quantitative PCR (qRT-PCR) will be used as a powerful tool with high sensitivity and specificity to quantitatively assess target periodontal bacteria in a period of 7 days. The investigators will also try to correlate the reduction of either total or respective bacteria with the improvements of clinical parameters, with an aim to uncovering the potential microbiological mechanism accounting for the efficacy of a therapy.

Arms:
arm 1: surface and root planning is performed in a single-visit, one-stage, full mouth pattern using periodontal ultrasonic scaler (Satelec, Mérignac, France) arm 2: Locally delivered minocycline is administered directly into the periodontal pocket up to the gingival margin of the selected teeth arm 3: surface and root planning+locally delivered minocycline is the combined administration of both surface and root planning and locally delivered minocycline.

Interventions: intervention 1: minocycline intervention 2: surface and root planning
Intervention descriptions: intervention 1: Periocline dental ointment intervention 2: surface and root planning

Protocol document: