Conditions: Hairy Cell Leukemia
Keywords: Biologic Therapy Monoclonal Antibody Immunotherapy Pseudomonas Exotoxin Targeted Therapy Hairy Cell Leukemia HCL
Allocation: NA | Intervention model: SINGLE_GROUP
Enrollment: 15
Healthy volunteers: no

Brief summary:
Background:

* About 80% of patients with hairy cell leukemia (HCL) have tumor cells that have a protein on their surface called cluster of differentiation 25 (CD25).
* The experimental drug LMB-2 is a recombinant immunotoxin that has been shown to kill leukemia and lymphoma cells with the CD25 protein. (A recombinant immunotoxin is a genetically engineered drug that has two parts - a protein that binds or targets a cancer cell, and a toxin that kills the cancer cell to which it binds.)

Objectives:

* To evaluate the safety and effectiveness of LMB-2 in patients with HCL whose cancer cells contain the CD25 protein.
* To evaluate the effects of LMB-2 on the immune system, determine how the drug is metabolized by the body and examine its side effects.

Eligibility:

-Adults with hairy cell leukemia whose tumor cells have CD25 on their surface

Design:

* Up to 27 patients may be included in the study.
* Patients receive an infusion of LMB-2 through a vein every other day for three doses (days 1, 3, 5), constituting one treatment cycle.
* Patients may receive up to six treatment cycles every 4 weeks unless their cancer worsens or they develop unacceptable side effects.
* Blood is drawn weekly for various tests.
* Before each cycle and in follow-up visits, disease status is evaluated with a physical examination, blood tests, chest x-ray and electrocardiogram.
* Before the first cycle, patients may have a computed tomography (CT) scan, echocardiogram (heart ultrasound test) and bone marrow biopsy. With the patient's permission, these tests may be repeated before other cycles also.

Detailed description:
Background: About 80% of patients with hairy cell leukemia (HCL) have malignant cells that express cluster of differentiation 25 (CD25) (Tac or Interleukin-2 receptor alpha (IL2Ra)). Normal resting B- and T-cells do not express CD25. LMB-2 is an anti-CD25 recombinant immunotoxin containing variable domains of MAb anti-Tac and truncated Pseudomonas exotoxin. A phase I trial at National Cancer Institute (NCI) found that the maximum tolerated dose (MTD) of LMB-2 was 40 microg/Kg intravenous (IV) given every other day for 3 doses (QOD x3). The most common adverse events were transient fever, hypoalbuminemia and transaminase elevations. In that trial, 4 of 4 patients with chemoresistant HCL had major responses, including one complete (CR) and 3 partial remissions. The patient with CR entered the trial transfusion dependent and now still has normal hemoglobin and platelet counts over 7 years later. Because HCL is more frequently cluster of differentiation 22 (CD22+) than CD25+ (100 vs 80%), HCL patients were subsequently treated with the anti-CD22 recombinant immunotoxin BL22 and no further HCL patients were treated with LMB-2. BL22 has induced 25 CRs out of 51 evaluable HCL patients. LMB-2 may be useful in patients incompletely responding to BL22, because it may distribute more evenly through extravascular sites of disease. Moreover, BL22 but not LMB-2 has caused hemolytic uremic syndrome (HUS) in 7 patients, 6 with HCL, and several of these patients could benefit by LMB-2. Thus, LMB-2 may be a useful and potentially lifesaving agent in patients who are unable to receive or who have not responded adequately to BL22.

Objectives: The purpose of this study is to determine the activity of anti-Tac(Fv)-PE38 (LMB-2) in patients with CD25-expressing hairy cell leukemia (HCL). The primary endpoint of this trial is response rate. We will also evaluate response duration, LMB-2 immunogenicity, pharmacokinetics, toxicity, and monitor soluble Tac levels in the serum.

Eligibility: Patients must have CD25+ HCL cells by flow cytometry, cytopenia or high circulating HCL count, prior treatment with or inability to receive BL22, prior treatment with cladribine, Eastern Cooperative Oncology Group (ECOG) Performance Status (PS) 0-2, at least 18 years old, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) grade 0-2, albumin grade 0-1, bilirubin less than or equal to 2.2, creatinine less than or equal to 1.4 or creatinine clearance greater than or equal to 50, lack of high levels of neutralizing antibodies, lack of anti-CD25 Mab therapy for 12 weeks and other systemic treatment for 4 weeks, no prior treatment with LMB-2, lack of other uncontrolled illness including 2nd malignancy, no human immunodeficiency virus (HIV) or hepatitis C positivity, no coumadin therapy, left ventricular ejection fraction (LVEF) greater than or equal to 45%, diffusing capacity of the lungs for carbon monoxide (DLCO) greater than or equal to 55%, and forced expiratory volume 1 (FEV1) greater than or equal to 60%.

Design: Patients will receive LMB-2 at 40 microg/Kg every other day (QOD) x3 at intervals of at least 25 days for up to 6 cycles. Retreatment is permitted in the absence of neutralizing antibodies or progressive disease. Patients in CR may receive 2 consolidation cycles, or 4 consolidation cycles if CR is with minimal residual disease.

Dose level: LMB-2 40 microg/Kg QOD x3

Expected Accrual: 5-10 patients/year, total of 25 patients

Arms:
arm 1: LMB-2 Infusion: 40 micro-g/Kg will be infused in 50 ml of 0.9% Sodium chloride (NaCl) and 0.2% albumin via over 30 minutes every other day for 3 doses. Patients may receive up to six treatment cycles every 4 weeks.

Interventions: intervention 1: Anti-Tac(Fv)-PE38 (LMB-2) Immunotoxin
Intervention descriptions: intervention 1: LMB-2 Infusion: 40 micro-g/Kg will be infused in 50 ml of 0.9% Sodium Chloride (NaCl) and 0.2% albumin via over 30 minutes every other day for 3 doses. Patients may receive up to six treatment cycles every 4 weeks.

Protocol document:
Prot_SAP_004.pdf: 
050823 
A Phase II Clinical Trial of Anti-Tac(Fv)-PE38 (LMB-2) Immunotoxin for CD25 Positive 
Hairy Cell Leukemia 
Protocol 06C0150  
NCT00321555 NCT00077922
Version Date: February 7, 2023 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
1
Abbreviated title: LMB-2 for HCL
NIH Protocol Number:  06-C-0150 
NCI CTEP Protocol Number: 7834
Version Date: 02/07/2023
NCT Number: NCT00077922
Closed to Accrual and Treatment with CTEP as of 10/20/2016
Title: A Phase II Clinical Trial of Anti-Tac(Fv)-PE38 (LMB-2) Immunotoxin for CD25 
Positive Hairy Cell Leukemia
NCI Principal Investigator: 
Robert J. Kreitman, M.D. 
Laboratory of Molecular Biology (LMB) 
Centers for Cancer Research (CCR)
National Cancer Institute (NCI), NIH 
9000 Rockville Pike, building 37/5124b
Bethesda, MD  20892
Phone 301-480-6187 
email kreitmar@mail.nih.gov
Drug Name:
anti-Tac(Fv)-PE38 (LMB-2)
IND Number:
6662
Sponsor:
CTEP
Manufacturer:
Monoclonal Antibody and 
Recombinant Protein facility, 
NCI, Frederick, MD
Supplier
NCI
Commercial Agents: none   


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
2
PRÉCIS 
Background.  
About 80% of patients with hairy cell leukemia (HCL) have malignant cells that express CD25 
(Tac or IL2Ra).  Normal resting B- and T-cells do not express CD25.  LMB-2 is an anti-CD25 
recombinant immunotoxin containing variable domains of MAb anti-Tac and truncated 
Pseudomonas exotoxin.  A phase I trial at NCI found that the MTD of LMB-2 was 40 µg/Kg  IV 
given every other day for 3 doses (QOD x3). The most common adverse events were transient 
fever, hypoalbuminemia and transaminase elevations.  In that trial, 4 of 4 patients with 
chemoresistant HCL had major responses, including one complete (CR) and 3 partial remissions.  
The patient with CR entered the trial transfusion dependent and now still has normal hemoglobin 
and platelet counts over 7 years later.  Because HCL is more frequently CD22+ than CD25+ 
(100 vs 80%), HCL patients were subsequently treated with the anti-CD22 recombinant 
immunotoxin BL22 and no further HCL patients were treated with LMB-2.  BL22 has induced 
25 CRs out of 51 evaluable HCL patients.  LMB-2 may be useful in patients incompletely 
responding to BL22, because it may distribute more evenly through extravascular sites of 
disease.  Moreover, BL22 but not LMB-2 has caused hemolytic uremic syndrome (HUS) in 7 
patients, 6 with HCL, and several of these patients could benefit by LMB-2.  Thus, LMB-2 may 
be a useful and potentially lifesaving agent in patients who are unable to receive or who have not 
responded adequately to BL22.  
Objectives.  The purpose of this study is to determine the activity of anti-Tac(Fv)-PE38 (LMB-2) 
in patients with CD25-expressing hairy cell leukemia (HCL).  The primary endpoint of this trial 
is response rate.  
Eligibility.  Patients must have CD25+ HCL cells by flow cytometry, cytopenia or high 
circulating HCL count, prior treatment with or inability to receive BL22, prior treatment with 
cladribine, ECOG PS 0-2, at least 18 years old, ALT and AST grade 0-2, albumin grade 0-1, 
bilirubin  ≤ 2.2, creatinine ≤ 1.4 or creatinine clearance ≥ 50, lack of high levels of neutralizing 
antibodies, lack of anti-CD25 Mab therapy for 12 weeks and other systemic treatment for 4 
weeks, no prior treatment with LMB-2, lack of other uncontrolled illness including 2nd 
malignancy, no HIV or hepatitis C positivity, no coumadin therapy, LVEF ≥ 45%, DLCO ≥ 
55%, and FEV1 ≥ 60%.
Design.  Patients will receive LMB-2 at 40 ug/Kg QOD x3 at intervals of at least 25 days for up 
to 6 cycles.  Retreatment is permitted in the absence of neutralizing antibodies or progressive 
disease.  Patients in CR may receive 2 consolidation cycles, or 4 consolidation cycles if CR is 
with minimal residual disease.  
Dose level:  LMB-2 40 ug/Kg QOD x3
Expected Accrual:  5-10 patients/year, total of 25 evaluable patients


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
3
TABLE OF CONTENTS
PRÉCIS............................................................................................................................................2
TABLE OF CONTENTS ................................................................................................................3
STATEMENT OF COMPLIANCE ................................................................................................5
1
OBJECTIVES ...........................................................................................................................5
1.1
Primary ...............................................................................................................................5
1.2
Secondary ...........................................................................................................................5
2
BACKGROUND ......................................................................................................................5
2.1
Hairy Cell Leukemia (HCL)...............................................................................................5
2.2
Salvage therapy for HCL....................................................................................................6
2.3
LMB-2 development ..........................................................................................................7
2.4
Rationale of Study Design................................................................................................13
2.5
Dose Determination..........................................................................................................14
3
ELIGIBILITY ASSESSMENT AND ENROLLMENT.........................................................14
3.1
ELIGIBILITY CRITERIA...............................................................................................14
3.2
Research Eligibility Evaluation........................................................................................15
3.3
Inclusion of Women and Minorities.................................................................................16
3.4
Recruitment Strategies .....................................................................................................16
3.5
Participant Registration and Status Update Procedures ...................................................16
4
STUDY IMPLEMENTATION...............................................................................................16
4.1
Study Design ....................................................................................................................16
4.2
Drug Administration.........................................................................................................17
4.3
Pharmacokinetic studies...................................................................................................19
4.4
On Study Evaluation ........................................................................................................20
4.5
Cost and Compensation....................................................................................................21
4.6
Toxicity Criteria ...............................................................................................................22
4.7
Retreatment ......................................................................................................................22
4.8
Supportive Care................................................................................................................22
4.9
Criteria for Removal from Protocol Therapy and Off Study Criteria ..............................23
4.10 Post Treatment Evaluation (off treatment but on study) ..................................................23
4.11 Off-study Criteria .............................................................................................................23
4.12 Lost to Follow-up .............................................................................................................24
5
HUMAN SUBJECTS PROTECTIONS .................................................................................24
5.1
Rationale for Subject Selection ........................................................................................24
5.2
Participation of Children ..................................................................................................24
5.3
Participation of Subjects Unable to Consent....................................................................24
5.4
Evaluation of Benefits and Risks/Discomforts ................................................................25
5.5
Risks/Benefits Analysis....................................................................................................27
5.6
Consent Process and Documentation ...............................................................................27
5.7
Sample Storage, Tracking and Disposition ......................................................................28
6
DOSING DELAYS/DOSE MODIFICATION.......................................................................29
6.1
No DLT ............................................................................................................................29
6.2
DLT ..................................................................................................................................29
6.3
Dose delay ........................................................................................................................29
7
ADVERSE EVENTS:  LIST AND REPORTING REQUIREMENTS (Sponsor).................30


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
4
7.1
Comprehensive Adverse Events and Potential Risks List(s) (CAEPRs) .........................30
7.2
Adverse Event Characteristics..........................................................................................32
7.3
Expedited Adverse Event Reporting ................................................................................33
7.4
Data Collection and evaluation ........................................................................................34
8
NIH REPORTING REQUIREMENTS / DATA AND SAFETY MONITORING PLAN....35
8.1
Definitions........................................................................................................................35
8.2
OHSRP Office of Compliance and Training / IRB Reporting.........................................35
8.3
NCI Clinical Director Reporting ......................................................................................36
8.4
NIH Required Data and Safety Monitoring Plan .............................................................36
9
REGULATORY AND OPERATIONAL CONSIDERATIONS ...........................................36
9.1
Study Discontinuation and Closure..................................................................................36
9.2
Quality Assurance and Quality Control ...........................................................................37
9.3
Conflict of Interest Policy ................................................................................................37
9.4
Confidentiality and Privacy..............................................................................................37
10
PHARMACEUTICAL INFORMATION ...........................................................................38
10.1 LMB-2 is an investigational recombinant immunotoxin. ................................................38
10.2 Premedications (abbreviated pharmaceutical section) .....................................................41
11
CORRELATIVE/SPECIAL STUDIES...............................................................................41
11.1 Assay for Detecting Neutralizing Antibodies to LMB-2. ................................................42
11.2 Bioassay for blood levels of LMB-2 ................................................................................43
12
STUDY CALENDAR .........................................................................................................46
13
MEASUREMENT OF EFFECT .........................................................................................48
13.1 Response criteria ..............................................................................................................48
13.2 Measurement of Response................................................................................................49
13.3 Confirmation of Response & Duration of Response........................................................49
14
DATA REPORTING / REGULATORY CONSIDERATIONS.........................................49
14.1 Procedures ........................................................................................................................50
14.2 Study Sponsor: CTEP.......................................................................................................50
15
STATISTICAL CONSIDERATIONS ................................................................................50
15.1 Study Design/Endpoints...................................................................................................50
15.2 Sample size/accrual rate ...................................................................................................50
16
REFERENCES ....................................................................................................................52


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
5
STATEMENT OF COMPLIANCE
The trial will be carried out in accordance with International Conference on Harmonisation Good 
Clinical Practice (ICH GCP) and the following: 
•
United States (US) Code of Federal Regulations (CFR) applicable to clinical studies (45 
CFR Part 46, 21 CFR Part 50, 21 CFR Part 56, 21 CFR Part 312, and/or 21 CFR Part 812) 
National Institutes of Health (NIH)-funded investigators and clinical trial site staff who are 
responsible for the conduct, management, or oversight of NIH-funded clinical trials have 
completed Human Subjects Protection and ICH GCP Training.
The protocol, informed consent form(s), recruitment materials, and all participant materials will 
be submitted to the Institutional Review Board (IRB) for review and approval.  Approval of both 
the protocol and the consent form must be obtained before any participant is enrolled.  Any 
amendment to the protocol will require review and approval by the IRB before the changes are 
implemented to the study.  In addition, all changes to the consent form will be IRB-approved; an 
IRB determination will be made regarding whether a new consent needs to be obtained from 
participants who provided consent, using a previously approved consent form.
1
OBJECTIVES 
1.1
PRIMARY 

To determine the response rate of LMB-2 in patients with CD25-positive Hairy Cell 
Leukemia (HCL).
1.2
SECONDARY

To describe the response duration.

To describe how blood levels of LMB-2 (AUC, Cmax) are related to toxicity. 

To describe how the development of neutralizing antibodies affects blood levels of LMB-
2 and toxicity.

To describe how soluble Tac-peptide (sIL2Ra) levels correlate with response to treatment 
with LMB-2.
2
BACKGROUND
2.1
HAIRY CELL LEUKEMIA (HCL)
Hairy cell leukemia (HCL) is a B-cell malignancy comprising 2% of all leukemias [1, 2], and is 
highly responsive but not curable with known therapy.  Patients present with pancytopenia and 
splenomegaly, and have malignant cells in the blood, marrow and spleen with eccentric kidney-
shaped spongiform nuclei and hair-like cytoplasmic projections.  The diagnosis was classically 
made by tartrate resistant acid phosphatase (TRAP) staining [3, 4].  More recently the diagnosis 
is most accurately made by fluorescent activated cell sorting (FACS) analysis (or flow 
cytometry), which demonstrates HCL cells strongly positive for B-cell antigens CD19, CD20, 
and CD22, and other antigens including CD103 (B-ly7) CD11c, CD25, and CD123 [5-7].  The 
differential diagnosis, which is important to consider in relapsed and refractory patients, includes 
splenic lymphoma with villous lymphocytes (SLVL), which may be distinguished from HCL by 
polar distribution of villous cytoplasmic projections, lack of pancytopenia, and CD25, CD103 
and/or CD123 negativity [7-9].  HCL variant (HCLv) is a disorder comprising about 20% of 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
6
HCL where the malignant cells may have bilobed nuclei, prominent nucleoli, and clumped 
malignant cells in the marrow.  The HCL cells may be CD25, CD103, CD11c and/or CD123 
negative and patients are primarily refractory to purine analogs and other therapies [6, 7, 10-12].
Cladribine as standard therapy for HCL.  Cladribine, also called 2-chlorodeoxyadenosine 
(CdA), is a purine analog inhibitor of ribonucleotide diphosphate reductase and DNA 
polymerase-a after phosphorylation by deoxycytidine kinase to 2-chloro-dATP [13].  Normally 
hairy cells are very sensitive because of high deoxycytidine kinase levels and low 5'-nucleotidase 
activity [14].  Saven et al. reported 91% complete remissions (CRs) and 6% partial responses 
(PRs) out of 349 evaluable patients, with only 24% CRs relapsing at a median of 30 months [15].  
A total of 205 of these patients plus 5 others were followed for at least 7 years, and 95% of 207 
evaluable patients achieved a CR lasting 8-172 (median 99) months and 34% of CRs relapsed 
[16].  The Group C phase II study of cladribine in HCL involved 979 patients treated by local 
physicians, and of 861 evaluable for response, there were 50% CRs and 37% PRs, although 13% 
of the PRs might have been CRs if bone marrow biopsy which was missed had been negative 
[17].  Nevertheless, the relapse free survival of 84% at 4 years was similar to the 79% reported 
from the Scripps study.  
Pentostatin as alternative standard therapy in HCL.  Pentostatin (2'-deoxycoformycin, DCF) 
is a purine analog inhibitor of adenosine deaminase (ADA), an enzyme elevated in acute 
lymphocyte leukemia cells but low in HCL cells [18].  The largest prospective trial of pentostatin 
in HCL was in the Intergroup study, where 72% CRs were reported in 154 initially treated plus 
87 treated after interferon failure [19, 20].  Relapse-free survival was 85% and 67% at 5 and 10 
years.  In a retrospective trial of 238 patients including 230 evaluable for response, 66% had CR, 
13% had CR not confirmed by bone marrow biopsy, and 17% had PR [21].  No trial has 
prospectively compared cladribine and pentostatin.  Pentostatin is not used as often as cladribine 
because of the need to dose every other week for several months instead of a single 1 week 
course, and pentostatin may have more gastrointestinal toxicity.
Prognosis with standard therapy in HCL.  Neither of the above-mentioned long term follow-
up studies of CdA show a plateau in the disease-free survival curve [15, 16].  Moreover, patients 
who are in CR after purine analog therapy were found to have PCR-detectable HCL [22, 23].  
Although purine analogs are highly effective even in relapsed patients with HCL, several large 
studies indicate declining CR rates and/or durations with each repeated course [15, 16, 24].  Lack 
of curability combined with declining efficacy with each retreatment points to eventual loss of 
ability to effectively treat the disease.  Clearly many patients may die of other causes, but those 
who live long enough may be predicted to progress and eventually die of HCL-related 
pancytopenia.  With over 15 years passing since the introduction of cladribine in the effective 
treatment of HCL, a significant number of patients with cladribine-resistant disease have 
accumulated over time, emphasizing the need for development of new approaches.  
2.2
SALVAGE THERAPY FOR HCL  
Splenectomy and interferon are older treatments for HCL which have a low rate of CR but can 
provide significant palliative benefit in selected cases [25-28].  Splenectomy can be 
accomplished safely in most patients by laparoscopy [29, 30].  While normal blood counts 
temporarily improve, there is increased hairy cell infiltration into the bone marrow, blood and 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
7
abdominal lymph nodes [31].  Interferon is sometimes recommended for relapsed HCL, 
particularly when cytopenias are severe and avoidance of myelotoxicity from purine analogs is a 
goal [32].  Fludarabine, though known for its excellent efficacy in CLL, is capable of inducing 
responses in HCL [33, 34].  Rituximab, targeting CD20, has been tested in 4 small trials of HCL 
patients [35-38], in which a total of 18 (30%) out of 60 patients achieved CR.  The 2 trials with 
the highest CR rates enrolled some patients without cytopenias [35, 38] and in one trial up to 12 
doses of rituximab were administered [38].  Thus, rituximab is an effective and well tolerated 
agent for HCL, at least in a minority of patients, but would not be considered standard therapy 
for this disease.  
2.3
LMB-2 DEVELOPMENT 
2.3.1
Anti-Tac
One strategy to enable a monoclonal antibody to kill cells is to use the antibody to block a 
growth factor receptor.  IL-2 is a growth factor for antigen-stimulated T cells and is responsible 
for T cell clonal expansion after antigen recognition. It is primarily produced by CD4+ T cells.  
The high-affinity IL-2 receptor (IL-2R) consists of 3 noncovalently associated proteins called 
Anti-Tac alpha (CD25), beta (CD122), and gamma (CD132), the latter two being members of the 
type I cytokine receptor family. The CD25 and CD122 chains are involved in cytokine binding, 
and the CD122 and CD132 chains are involved in signal transduction. CD25, originally called 
p55 or the Tac antigen (for T cell activation), is a 55-kD polypeptide that normally appears when 
T cells are activated.  It is usually not expressed on resting T-cells, B-cells or monocytes [39].  
However, CD122-CD132 (intermediate-affinity IL-2R) is expressed on resting (naïve) T cells 
and binds IL-2 at 1 x 10-9 M concentration. When T cells are stimulated and produce CD25 
chains the affinity of IL-2 increases to 1 x 10-11 M. Chronic T cell stimulation can lead to the 
shedding of a 45-kD piece of CD25 into the blood.  The serum soluble Tac (sCD25) level can 
then be followed as a marker of strong antigenic stimulation such as in rejection of a transplanted 
organ [40].
Anti-Tac is a monoclonal antibody which binds to IL2R (CD25) with high affinity blocking the 
interaction of IL2 with the IL2R [41-45].  Unmodified humanized anti-Tac monoclonal antibody 
(daclizumab, NSC-277524, IND#2091) was tested in 19 ATL patients (NIH 83-C-0023) to 
determine if it would block IL2 from stimulating the malignant cells [46, 47].  There was no 
significant toxicity.  Six patients had remissions including 4 partial and 2 complete remissions 
lasting from one to more than 43 months after onset of therapy.  Five of the responding patients 
relapsed following therapy.  Relapses were not due to loss of CD25 expression by the ATL cells, 
but rather to loss of dependence of the ATL cells on IL2 for their proliferation.  Thus, CD25 
remains a target for immunotherapy.  
2.3.2
PE38
The full-length 613 amino acid PE protein contains three functional domains which are 
necessary for cellular intoxication [48, 49].  Domain Ia (amino acids 1-252) is the binding 
domain, domain II (amino acids 253-364) is for translocating the toxin to the cytosol and domain 
III (amino acids 400-613) contains the ADP ribosylating enzyme which inactivates elongation 
factor 2 (EF-2) in the cytosol and results in cell death.  The function of domain Ib (amino acids 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
8
365-399) is unknown.  A current model of how PE kills cells includes the following steps:  1)  
The C-terminal residue (lysine-613) is removed by a carboxypeptidase in the plasma or culture 
medium [50].  2)  Domain Ia binds to the a2 macroglobulin receptor, present on animal cells 
[51].  3)  After internalization at low pH, domain II is proteolytically cleaved between amino 
acids 279 and 280 by furin [52-54].  4)  The disulfide bond between cysteines 265 and 287 
which joins the two fragments is reduced, producing an N-terminal fragment of 28 kDa and a C-
terminal fragment of 37 kDa.  5)  Amino acids 609-612 (REDL) bind to an intracellular sorting 
receptor which transports the 37 kDa carboxy terminal fragment from the transreticular Golgi 
apparatus to the endoplasmic reticulum [55, 56].  6)  Amino acids 280-313 mediate translocation 
of the toxin to the cytosol [57, 58].  7)  The ADP ribosylating enzyme composed of amino acids 
400-602 inactivates EF-2 [59].  PE40 is a truncated derivative of PE which is missing the 
binding domain and hence will not bind specifically to cells unless attached to an antibody or 
growth factor [48, 60].  PE38 is a truncated version of PE40 which is missing amino acids 365-
380.  While PE40 and PE38 have similar activities [61, 62], we have preferred to use PE38 
because it is slightly smaller and is missing a disulfide bond that impairs refolding the protein.  
2.3.3
The Recombinant Immunotoxin
To target cells with a single-chain protein containing the antigen binding domains of anti-Tac, 
the variable domains of the antibody (VH and VL) were fused together with the peptide linker 
(G4S)3 and the resulting Fv fragment of anti-Tac was fused to PE40 [63].  Anti-Tac(Fv)-PE40 
was extremely cytotoxic with an IC50 of 0.15 ng/ml toward HUT-102 cells [63] and 0.05-0.1 
ng/ml toward activated human T-cells [62, 64].  To determine if malignant cells in patients have 
enough receptors and metabolize the toxin effectively enough to be sensitive to anti-Tac(Fv)-
PE40, we tested ATL cells from the blood of 38 patients and from the lymph nodes of 5 patients.  
All samples were sensitive to anti-Tac(Fv)-PE40, with IC50's of 0.03-16 ng/ml [62, 65-67].  
Anti-Tac(Fv)-PE40 was shortened slightly by removing amino acids 365-380, resulting in anti-
Tac(Fv)-PE38 (LMB-2).  The cytotoxic activity of anti-Tac(Fv)-PE38 (LMB-2) appears identical 
to that of anti-Tac(Fv)-PE40 toward cell lines and fresh ATL samples [62].  Importantly, it was 
shown that these recombinant anti-CD25-immunotoxins are still cytotoxic in the presence of 
soluble CD25 concentrations as high as those expected in ATL patients [67].  This suggested 
that even in ATL patients, whose sera have the highest levels of soluble CD25, anti-Tac(Fv)-
PE38 (LMB-2) should not be prevented by soluble CD25 from reaching the malignant cells.
2.3.4
Preclinical studies of LMB-2 in vivo
A mouse model of a human CD25 positive malignancy was produced by the subcutaneous 
injection in nude mice of ATAC-4 cells [68].  These cells are A431 epidermoid carcinoma cells 
that have been transfected with the gene encoding CD25, and contain 2 x 105 CD25 sites/cell 
[62].  Mice began treatment with LMB-2 four days after ATAC-4 cell injection, when 
subcutaneous tumors became established (32-86 mm3).  Ninety-100% tumor regressions were 
observed in 2 of 5 mice receiving 30 µg/Kg i.v. QD X 3, and in 5 of 5 mice receiving 60 µg/Kg 
i.v. QD X 3.  These doses were respectively 5 and 10% of the mouse LD50 [68].  When 
administered to mice every other day, complete tumor regressions could be obtained in 5 of 5 
mice receiving 100 µg/Kg i.v. days 4, 6 and 8.  The LD10 and LD50 are 200 and 257 µg/Kg 
every other day for 3 doses.  The cause of death on necropsy of the mice was liver damage.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
9
Cynomolgus monkeys were used to determine the safety and pharmacokinetics of LMB-2, since 
anti-Tac reacts with primate but not murine CD25.  In a GLP pharmacokinetic study, the 
elimination of LMB-2 from the serum followed biphasic kinetics, with a T1/2 of 45 minutes 
and a T1/2 of 127 minutes.  In a GLP toxicology study, 4 Cynomolgus monkeys received 20 
µg/Kg days 1, 3 and 5 with no significant toxicity.  Four monkeys were then given 300 µg/Kg 
days 1, 3 and 5 and experienced dose-limiting toxicity with anorexia and 2 to 4-fold 
transaminase elevations.  One of two monkeys (male) autopsied on day 7 in this high-dose group 
had hepatomegaly, mild diffuse hepatocyte vacuolation, testicular and epididymal degeneration, 
and leukocytosis and vascular inflammation of the skin.  The other monkey (female) had a ~0.5 
X 0.8 mm area of myocardial degeneration.  Of the four high-dose monkeys, the one with 
hepatocyte vacuolation had the mildest (up to 2-fold) transaminase elevations.  The two 
remaining high-dose monkeys were sacrificed on day 51. One of these (male) was found to have 
a ~0.4 X 0.4 mm area of myocardial degeneration and testicular and epididymal degeneration.  
The other (female) was found to have a myocardial parasite.  No monkeys had detectable CPK-
MB positivity or LDH I/II isoenzyme ratio greater than one at any time point.  After the 
toxicology study was completed, it was reported by a group at Parke-Davis Research Institute 
that untreated wild-caught Cynomolgus monkeys have high rates of various pathologic lesions.  
In particular, of 62 male and 62 female animals, 45.2% of males and 48.4% of females had 
cardiac lesions, 58.1% of males and 54.8% of females had hepatic lesions, 31.4% had skin 
lesions and 13% of males had immature testicular and accessory sex organ histology [69].  
Unpublished data from these investigators indicated that 6.4% of the males and 14.5% of the 
females had myocardial degeneration and/or necrosis, 8.1% of the males and 3.2% of the females 
had hepatic necrosis, 4.8% of males and 1.6% of females had hepatic vacuolation, 6.4% of males 
and females had mixed cell infiltrates in skin, 1.6% of males and 3.2% of females had 
inflammation in the skin and 12.9% of males had immature testes.
2.3.5
Phase I trial of LMB-2 (NIH #96-C-0064)
LMB-2 induced responses in patients with CD25+ chemotherapy-resistant hematologic 
malignancies, including 4 with hairy cell leukemia (1 CR, 3 PR) and one PR each with adult T-
cell leukemia (ATL), Hodgkin's disease (HD), chronic lymphocytic leukemia (CLL), and 
cutaneous T-cell lymphoma (CTCL) [70, 71].  The HCL patient with CR had a Hgb as low as 
3.8 prior to transfusions and continues to have a normal Hgb more than 7 years later.  The 3 HCL 
patients with PR had limited retreatment due to pneumonia, DLT, and neutralizing antibodies.  
The published Phase I results included 35 patients (age range 24-79), 11 with HD, 6 with B-cell 
lymphoma, 8 with CLL, 4 with HCL, 3 with PTCL, 1 with CTCL, and 2 with ATL.  Of these 35 
patients, 22 received 1 cycle only, 8 received 2 cycles, 2 received 3 cycles, and 1 each received 
4, 5, and 6 cycles.  Twenty patients developed anti-PE38 neutralizing antibodies and 9 of those 
patients also developed HAMA anti-bodies.  Twenty-nine of the 35 patients received a starting 
dose of  10µg/Kg  QOD x 3.  Data discussed below includes 4 patients not in the phase I report, 
who had ATL (#36), HD (#37), CTCL ((#38) and NHL (#39).  Patient #36 received 50 ug/Kg 
QOD x3 and patients #37-39 received 40 ug/Kg QOD x3.  Of these 4 patients, patients #38-39 
each received a 2nd cycle and patient #38 developed neutralizing antibodies.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
10
LMB-2 related toxicity with at least one cycle of LMB-2 was seen at  10µg/Kg  QOD x 3 and 
was reversible.  Please see Table 1 below for a list of toxicities.  The common toxicity criteria 
(CTC) of the NCI were used to grade toxicity.  Dose Limiting Toxicity (DLT) was defined as at 
least grade 3 toxicity (attribute possible, probable, or definite), but the following exceptions were 
not considered to be dose-limiting: (1) transaminases elevations of 5-20 times normal, (2) 
bilirubin 1.5-2.2 times normal, (3) fever that was well tolerated and did not result in an 
interruption in therapy, (4) hematologic toxicity in leukemic patients, (5) grade 3 hematologic 
toxicity in nonleukemic patients, and (6) abnormal coagulation profiles in patients who were 
receiving anticoagulant therapy or who had preexisting coagulation abnormalities.
DLT was observed in two of three patients at the 63 µg/Kg  QOD x 3 dose level.  The first 
patient (with HD) had asymptomatic grade 4 AST and grade 3 ALT elevations.  The second 
patient (with HCL) developed grade 3 diarrhea, grade 2 fever, nausea, and vomiting, and grade 4 
cardiomyopathy on day 5.  The patient’s cardiac function returned to normal by day 7.  FACS of 
peripheral blood on day 9 indicated a large number of dead tumor cells.  We concluded that the 
cardiomyopathy was either cytokine mediated or due to direct LMB-2 toxicity on the heart.  The 
50 µg/Kg  dose level was dose limiting in 1 of 6 patients (patient #27 with PTCL) due to an 
allergic reaction.
The most common toxicities were transient fever and transaminase (ALT, AST) elevations, 
usually grade 1 or 2.   Grade 3 transaminase elevations were not associated with impaired liver 
function as assessed by PT, bilirubin, and fibrinogen levels.  Grade 1 nausea and vomiting could 
not be correlated with the degree of transaminase elevations.  The transaminase elevations were 
never observed to increase after day 8 of each cycle in any patient.  In all patients with grade 3-4 
elevations, the AST and ALT levels resolved to 40 U/L.
Fever (22/29 patients) typically occurred within hours of the first dose and did not recur after the 
second and third doses.  Often it recurred on subsequent cycles after the first dose.  Eighteen of 
22 patients with normal pretreatment albumin levels (3.7g/dL) experienced hypoalbuminemia, 
11 patients with grade 1 and 7 with grade 2.  Most of these patients did not develop significant 
weight gain and none had symptomatic pulmonary edema.  Reversible grade 1 drug-related renal 
toxicity was observed in 3 patients.  
For pharmacokinetic studies, dilutions of patient plasma were tested in cytotoxicity assays 
against a cell line (SP2/Tac) and compared to a cytotoxicity standard curve created with known 
amounts of LMB-2.  In 12 patients treated at the MTD (40 µg/kg), peak plasma levels after cycle 
1 were 340-1040 (median 560) ng/ml, the AUCs were 48-257 (median 123) ug-min/ml, the half-
lives were 185-322 (median 216) min, and the clearances were 14-79 (median 25) ml/min).  The 
median values for the 4 patients with HD were 620 ng/ml, AUC 155 ug-min/ml, half-life 193 
min, and clearance 19 ml/min.  
Patient #36 with ATL was treated at 50 µg/Kg QOD x3 and experienced a reversible but dose-
limiting syndrome similar to patient #30, with 3rd spacing and muscle edema causing grade IV 
CK elevation and hypoventilation leading to supraventricular tachycardia and respiratory failure.  
Associated toxicity included grade III hypoalbuminemia, grade II ALT, grade III AST, grade II 
acidosis, grade I fever, grade I GGTP, grade II lipase, grade I thrombocytopenia grade III 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
11
hypotension and grade II weight gain.  This patient had a muscle biopsy which ruled out necrosis 
or inflammation, indicating that the CK elevation was from passive muscle fiber edema and 
stretching.  Due to this DLT event (2 out of 6 patients at this level now had DLT), the dose level 
was reduced to 40 µg/Kg QOD x3.
In phase II testing, LMB-2 has been administered to 5 CLL patients without DLT at 40 ug/Kg 
QOD. Patient CL03 had 6 cycles and a PR. Patient CL04 had a good response in lymph nodes 
but not in the peripheral blood after 2 cycles. He had grade II weight gain and CPK elevation 
with cycle 1, but much less toxicity with cycle 2. He did have a pneumonia after cycle 2 and on 
day 27 was diagnosed with bilateral occipital infarcts related to a documented patent foramen 
ovale.  On a phase II CTCL protocol, 1 patient was enrolled at 40 ug/kg QOD x3 who had 
transient grade IV CPK elevation as his only DLT, so the dose level was lowered for future 
CTCL patients to 30 ug/Kg QOD x3.
Several patients treated with LMB-2 have developed completely reversible allergic reactions 
other than rash, associated with immunogenicity to LMB-2.  Immunogenicity to the toxin 
domains of similar immunotoxins BL22, HA22 and SS1P is not associated with allergic 
reactions other than rash, suggesting relationship to the targeting domain, anti-Tac(Fv).  Grade 3-
4 allergic reactions observed during phase I testing included grade 3 bronchospasm during the 1st 
dose of cycle 2 in a patient with HD, and grade 4 anaphylaxis during the 1st dose of cycle 1 in a 
patient with PTCL (73).  The patient with anayphylaxis during cycle 1 of LMB-2 had prior 
humanized anti-Tac Mab (Zenapax, daclizumab) treatment and was in retrospect found to have 
human anti-human IgG antibody (HAHA), suggesting that the idiotype of LMB-2, which is also 
present in humanized anti-Tac Mab, plays a role in both immunogenicity and allergic reactions.  
Of 9 CTCL patients who have undergone phase 1 or 2 testing, one had an allergic reaction during 
cydle 2, associated with an unusual feeling in the throat.  Of 11 HCL patients who have 
undergone phase 1-2 testing, 2 had syncopal episodes during retreatment, one of whom noted an 
identical episode with rituximab which was subsequently prevented with steroids.  The level of 
neutralizing antibodies prior to the cycle causing allergic reactions was either low or 
undetectable and did not correlate with allergic reaction.  To prevent these allergic reactions, 
CTCL patients will receive steroid prophylaxis with a single dose of dexamethasone 20 mg i.v. 2 
hours prior to the first dose of each retreatment cycle of LMB-2.
In summary, LMB-2 is well tolerated at the MTD except for 1 patient with CTCL.  The most 
common toxicity, transaminase elevation, was reversible and never associated with any other 
evidence of hepatic dysfunction.  All other toxicities at the MTD were also transient, resolving 
within a few days to weeks after treatment.
Table 1
LMB-2-related cycle-1 toxicities for patients treated at 10µg/Kg  QOD x 3 (phase I patients 7-
39) 
Toxicity
 Dose Level:  Total patients (total Grade 3 or 4) 
10
20
30
40
50
63
Total treated
3
3
5
12
7
3
Transaminases
2
2
5(1)
11(3)
7(3)
3(2)
Fever
0
2
5
10
6(1)
3(1)


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
12
Myalgia
2
2
1
7
1
1
Alkaline phosphatase 0
0
0
3
0
3
Thrombocytopenia
0
1
0
3(1)
2
1
Hypoalbuminemia
1
1
4
7
4(1)
2
Hypotension
0
0
0
4
4(1)
2
Nausea/Vomiting
0
0
0
7
2
3
Diarrhea
0
0
0
1
1
1(1)
Pericardial effusion
0
1
1
2
0
2
Weight Gain
1
0
1
5
2
2
Allergy
0
0
0
2
2(1)
0
Increased Creatinine 0
0
0
3
2
0
Proteinuria
0
0
0
2
1
0
Cardiomyopathy
0
0
0
0
0
1(1)
Total Evaluated
3
3
5
12
7
3
Table 2 
Phase I LMB-2-related toxicities for the 12 patients treated at the MTD (40 µg/Kg )
Toxicity
  
      Grade                  Total # of patients
1
2
3
Transaminases 
6
2
3
11
Fever
3
7
10
Myalgia
5
2
7
Alkaline phosphatase 2
1
3
Thrombocytopenia 
2
1
3
Hypoalbuminemia
2
5
7
Hypotension
4
4
Nausea
5
2
5
Vomiting 
1
1
2
Creatinine/Proteinuria3
3
Pericardial effusion 
2
2
Weight Gain
3
2
5
Diarrhea
1
1
2.3.6
LMB2 Test Dose
All patients on the Phase I trial received a 10 µg test dose before the first dose of LMB2 on day 1 
of each cycle.  The test dose was administered 30 minutes prior to the LMB2 treatment dose 
which was 30 minutes after pre-medication.  There was no correlation between patient tolerance 
of test doses and LMB2 treatment.  Notably, no patients were taken off treatment due to test dose 
reactions. Furthermore, none of the patients who had allergic toxicity had sustained reactions to 
the test dose.  Due to this lack of utility, test doses will not be administered on this trial.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
13
2.3.7
Efficacy of anti-CD22 recombinant immunotoxin in HCL
Fifty-three patients with hairy cell leukemia (HCL) previously treated with CdA were treated 
with BL22, 31 in phase I testing, 2 by special exemption, and 20 in phase II testing.  The 
complete remission rate in phase I was 19 (61%) out of 31 patients, and 6 (19%) had partial 
response (PRs) [72, 73].  The median (range) duration of CR was 36 (5-73) months and 7 (37%) 
patients are still in CR at a median of 51 (range 35-73) months.  Of 30 with cytopenias, 22 (73%) 
achieved hematologic remission (HR), with neutrophils, platelets, and hemoglobin at least 1500, 
100,000 and 11.  Nine (41%) are still in HR at a median of 50 (35-73) months.  Of 19 evaluable 
HCL patients on phase II, 8 (42%) achieved CR, 3 (16%) PR, and 3 (16%) who are not yet in CR 
are still under retreatment.  Only 2 phase II patients have relapsed from CR, both of whom had 
no consolidation cycles of BL22.   BL22 is safe at 40 ug/Kg QOD x3 for cycle 1, with no BL22-
related SAEs observed in 12 of 12 patients in phase I, 2 of 2 patients on special exemption, and 
20 of 20 patients on phase II.  However, with retreatment at 30-50 ug/Kg QOD x3, 6 HCL 
patients have had a reversible hemolytic uremic syndrome (HUS), during cycles 2 or 3.  The first 
5 HUS cases in HCL were treated with plasma exchange, but the 6th patient was not and still had 
complete recovery.  HUS has not been observed with LMB-2 or any other PE38-containing toxin 
besides BL22, indicating that the mechanism of HUS is mediated by both the toxin and CD22-
binding domains.  HUS due to BL22 has also been observed in 1 patient each with non-
Hodgkin's lymphoma and CLL, indicating that the syndrome is probably not dependent on the 
HCL phenotype.  High levels of neutralizing antibodies were observed in 1 out of 31 phase I 
HCL patients after cycle 1, but with retreatment of up to 30 cycles over nearly 5 years in some 
patients, 11 (35%) made high levels of neutralizing antibodies overall.  Neutralizing antibodies 
to BL22 include antibodies to the toxin, and such patients would not be eligible for LMB-2.  
However, only 1 out the 6 HCL patients with HUS made neutralizing antibodies to BL22, and 
thus the other 5 patients, who are ineligible for BL22 because of prior HUS, would be eligible 
for LMB-2.
2.4
RATIONALE OF STUDY DESIGN 
2.4.1
CD25 expression in HCL.  CD25 is expressed on about 75-80% of HCL samples [6].  
LMB2 is a recombinant immunotoxin that targets CD25.  
2.4.2
Response of HCL to LMB-2.  In the phase I dose-escalation trial of LMB2, there were 1 
CR and 3 PRs out of 4 HCL patients [70, 71].  Several of these responders could not be 
effectively retreated either because of neutralizing antibodies or disease-related 
infections.
2.4.3
Need for LMB-2 in HCL.  BL22 is clearly the preferred agent for HCL due to 20-25% of 
patients who have CD25 negative HCL cells.  Thus HCL patients eligible for LMB-2 
would have already received BL22 and not be eligible or appropriate for further BL22.  
Such patients include 2 groups:
2.4.3.1 Patients who had HUS from BL22.  Currently 8 CD25+ HCL patients have had HUS 
from BL22.  Several of these might be appropriate for further BL22 at a lower dose 
level, but since LMB-2 has never caused HUS, the dose they could receive of LMB-2 
would be significantly higher and hence their chance of response much better.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
14
2.4.3.2 Patients who did not completely respond to BL22 despite multiple cycles.  A possible 
reason for lack of CR could be that since CD22 is expressed at such high levels in HCL 
(up to 100,000 sites/cell), patients with significant tumor burden could have very 
limited distribution of BL22 to all sites of tumor.  HCL, while being very sensitive to 
LMB-2 [74], express only about 1300-17,000 sites/cell and the half-life is slightly 
longer than BL22.  A total of 6 out of 31 patients on phase I and 5 out of 18 on phase II 
might have benefited from LMB-2 due to improved distribution.  Several of these 
patients are still alive without neutralizing antibodies and might benefit by LMB-2.
2.5
DOSE DETERMINATION  
The MTD established in the phase I trial of LMB-2 in 12 patients was 40 ug/Kg QOD x3.  In 
phase II testing, Four out of 5 patients with CLL tolerated this dose level without DLT, and one 
patient with CTCL had grade IV CPK elevation without other evidence of DLT.  This led to a 
lowering of the phase II dose level in the CTCL trial to 30 ug/Kg QOD x3.   Because HCL is 
more like CLL in having an intravascular component, we plan to use the 40 ug/Kg QOD x3 dose 
level.  LMB-2 will be given every 4 weeks for up to 6 cycles.
3
ELIGIBILITY ASSESSMENT AND ENROLLMENT
3.1
ELIGIBILITY CRITERIA
3.1.1
Inclusion Criteria
3.1.1.1 Histopathological evidence of CD25+ HCL confirmed by the NIH pathology department.  
This will require a monoclonal population of peripheral malignant lymphocytes that are 
CD25 positive by fluorescence activated cell sorting (FACS) with anti-CD25 antibody.  
Positive expression in a FACS assay is defined as more than 2 times the mean 
fluorescence intensity (MFI) of the control antibody by FACS.  HCLv (HCL variant) is 
usually CD25 negative, and eligibility would require CD25+ HCLv.
3.1.1.2 At least one of the following indications for treatment: neutropenia (ANC <1000 
cells/ul), anemia (Hgb <10g/dL),  thrombocytopenia (Plt <100,000/ul), an absolute 
lymphocyte count of >20,000 cells/ul or symptomatic splenomegaly.
3.1.1.3 Previous treatment with or inability to receive BL22 or HA22 recombinant immunotoxin. 
Patients must have had at least 2 prior systemic therapies, including 2 courses of a 
purine nucleoside analog (PNA), or 1 course of either rituximab or BRAF inhibitor 
following a single prior course of PNA.
3.1.1.4 ECOG performance status of 0 – 2.
3.1.1.5 At least 18 years old.
3.1.1.6 Understand and give informed consent.
3.1.1.7 A negative pregnancy test in female patients of childbearing potential.  Women must not 
be breast-feeding (see section 5.1.1.1). 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
15
3.1.1.8 ALT and AST ≤5-times the upper limits of normal.  Albumin ≥3.0 gm/dL.  Total 
bilirubin ≤2.2 mg/dL.
3.1.1.9 Creatinine ≤1.4 mg/dL or creatinine clearance ≥50 ml/min. 
3.1.1.10 Serum that neutralizes ≤75% of the activity of 1 µg/mL of LMB-2 using a bioassay.
3.1.1.11 No systemic cytotoxic chemotherapy within 4 weeks of enrollment or systemic steroids 
(except stable doses of Prednisone ≤20 mg/day, or up to 4 doses of steroid given for 
non-therapy reasons) within 4 weeks of enrollment.  
3.1.1.12 No anti-CD25 monoclonal antibody therapy within 12 weeks of enrollment.
3.1.1.13 No prior treatment with LMB-2.
3.1.1.14 Patients may not be receiving any other investigational agents.
3.1.1.15 Patients should not have uncontrolled intercurrent illness including, but not limited to, 
symptomatic congestive heart failure, unstable angina pectoris, cardiac arrhythmia, or 
psychiatric illness/social situations that would limit compliance with study 
requirements.
3.1.2
Exclusion Criteria
3.1.2.1 Patients who have HIV or hepatitis C.  Patients would not be excluded for hepatitis B 
surface antigen positivity if on Lamivudine.  
3.1.2.2 Patients receiving coumadin.
3.1.2.3 Patients with a left ventricular ejection fraction of < 45%.
3.1.2.4 Patients with a DLCO <55% of normal or an FEV1 <60% of normal, based on either NIH 
or USA normal ranges.
3.1.2.5 Patients who have an active 2nd malignancy requiring systemic treatment. 
3.2
RESEARCH ELIGIBILITY EVALUATION

A blood sample will be submitted to the NIH pathology department for analysis of CD25 
expression (an eligibility criterion) by FACS analysis. This analysis will be done in the 
Laboratory of Pathology, NIH (Anatomic Pathology Dept., CC), a College of American 
Pathologists (CAP) and CLIA-approved laboratory, by standardized diagnostic 
techniques. 

Complete history and physical examination with documentation of measurable disease 
(lymph nodes, spleen, liver), stage, and performance status within 2 weeks before starting 
LMB-2.  Physical examination should record the diameter, in two planes, of the largest 
palpable nodes in each of the following sites: cervical, axillary, supraclavicular, inguinal, 
and femoral.  Physical examination should record liver and spleen size as determined by 
measurement below the respective costal margin. 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
16

ECG and CXR within 3 days before starting LMB-2. 

CT scan of the chest, abdomen, and pelvis, or abdominal MRI, within 4 weeks before 
starting LMB-2.

Serum anti-LMB-2 antibody assay, HIV, Hepatitis B surface antigen, and hepatitis C 
screen within 2 months before starting LMB-2.

Laboratory evaluation within 2 weeks before starting LMB-2 will include 
CBC/differential, acute care panel (electrolytes, glucose, BUN, creatinine), hepatic panel 
(AST, ALT, Alkaline phosphatase, total and direct bilirubin), albumin, LDH, PT, Beta 2 
microglobulin (B2M), serum protein electrophoresis (SPEP), urinalysis, and a 24 urine 
creatinine clearance and total protein. A CBC/differential, acute care panel, hepatic panel, 
and albumin must be done within 3 days of starting cycle 1.

Urine or serum pregnancy test within 72 hours before starting LMB-2 in women with 
childbearing potential.

Bone marrow biopsies and echocardiogram with left ventricular ejection fraction 
calculation, and stress test, will be done on all patients within 2 months before starting 
LMB-2.
3.3
INCLUSION OF WOMEN AND MINORITIES
Inclusion of Women and Minorities.  No eligible women and minorities will be excluded from 
the protocol.  There is no basis for exclusion of otherwise eligible patients on the basis of age, 
gender or race.  
3.4
RECRUITMENT STRATEGIES
This protocol may be abstracted into a plain language announcement posted on NIH websites 
and on NIH social media platforms.
3.5
PARTICIPANT REGISTRATION AND STATUS UPDATE PROCEDURES 
Registration and status updates (e.g., when a participant is taken off protocol therapy and when a 
participant is taken off-study) will take place per CCR SOP ADCR-2, CCR Participant 
Registration & Status Updates found at: 
https://ccrod.cancer.gov/confluence/pages/viewpage.action?pageId=73203825.
3.5.1
Screen Failures
Screen failures are defined as participants who consent to participate in the clinical trial but are 
not subsequently assigned to the study intervention or entered in the study. A minimal set of 
screen failure information is required to ensure transparent reporting of screen failure 
participants, to meet the Consolidated Standards of Reporting Trials (CONSORT) publishing 
requirements and to respond to queries from regulatory authorities. Minimal information 
includes demography, screen failure details, and eligibility criteria.
4
STUDY IMPLEMENTATION
4.1
STUDY DESIGN
This is a Phase II study to test the response of patients with HCL to LMB-2.  LMB-2 will be 
given every 28 days (minimum of 25 days) for up to 6 cycles. No patient whose serum 
neutralizes > 75% of the activity of 1 µg/mL of LMB-2 will be retreated.  In patients who 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
17
achieve a CR with minimal residual disease (MRD), defined as positive immunohistochemistry 
of bone marrow biopsy or flow cytometry of blood, no more than 4 additional cycles of LMB-2 
will be administered.  Patients with CR without MRD may receive 2 additional cycles.  Patients 
who relapse after >2 months of a CR or PR are eligible for retreatment with LMB-2 alone on the 
same schedule if they still meet initial eligibility criteria and were not removed from study due to 
toxicity, provided that the study is still open and the LMB-2 supply permitting.  Stopping rules in 
this case will be the same as those during initial LMB-2 administration.  Patients will be treated 
with LMB-2 as described below.  Response and duration of response will be determined as 
described in section 13. 
4.2
DRUG ADMINISTRATION
LMB-2 Infusion: 40 µg/Kg will be infused through a peripheral I.V. or central line in 50 ml of 
0.9% NaCl and 0.2% albumin via a PAB container over 30 minutes every other day for 3 doses 
(QOD x 3).  Additional IV fluid will be given as described below.  The first cycle will be 
administered on the Oncology Inpatient Unit.  If treatment is tolerated well, then subsequent 
doses can be administered on an outpatient basis.
Premedication: Patients will be medicated with 25 mg hydroxyzine and 150 mg ranitidine orally 
1 hour prior to and 8 hours after each dose.  Acetaminophen 650 mg P.O. will be given every 6 
hours for 4 doses starting 1 hour prior to each LMB-2 dose.  Dexamethasone 20 mg i.v. will be 
given 2 hours prior the 1st dose of each retreatment cycle. While the times of concomitant 
medications are recommended, the actual time of administration should be documented 
particularly when they differ. Emergency medications such as epinephrine and diphenhydramine 
should be available in the area where the patients will receive the LMB-2 infusion for treatment 
of an allergic reaction.  Emergency equipment including oxygen should be available in the 
patient’s room.
I.V. Fluid:  Patients will receive fluid prophylaxis, consisting of 1000 ml of D5/0.45% NaCl over 
2-4 hours prior to LMB-2 and another 1000 ml of D5/0.45% NaCl over 2-4 hours after the LMB-
2 infusion is completed.  (This is approximately the same amount of IVF given to the last 4 
patients on the phase I protocol.  However, it is in a more simple administration format). Once 
the first dose of each cycle is given, patients will receive normal saline, at least 40 cc/hr until day 
5-8 when not receiving another type of I.V. fluid.
4.2.1
Vital signs of inpatients will be obtained at the beginning of infusion, at 15 minutes, &   
at the end of infusion, then every 60 minutes for 2 hours, then as per unit routine.  Daily 
orthostatic blood pressure, weights, I/Os, and physical exam will be done.  Daily 
orthostatic blood pressure will be measured after having the patient in supine and 
standing position for at least 2 minutes.  If standing SBP decreases from supine SBP by > 
20 mmHg, then another standing blood pressure measurement will be done at least 3 
minutes after the first.  Please see section 4.8 for management of hypotension.  Vital 
signs of outpatients will be obtained at the beginning of infusion, at 15 minutes, & at the 
end of infusion, then every 60 minutes for 2 hours.  Patients will be monitored for 
peripheral edema and orthostatic hypotension at least daily.  Daily weights will also be 
recorded.  Please see section 4.8 for management of hypotension.  Determination of DLT
DLT is defined as LMB-2-related grade III-V toxicity, with exceptions below.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
18
4.2.2
Definition of VLS 
Definition of Vascular Leak Syndrome (as stipulated by CTEP to be utilized until December 31, 
2010):  As specified by CTC 3.0, grade II VLS includes symptoms of fluid retention; this 
protocol further defines that if weight gain is the only feature of VLS in a patient, it should be 
grade I-II to be classified as grade II VLS.  If a patient requires more than an hour's worth of 
hydration at 20 ml/Kg/hour for hypotension, then the patient will be considered to have grade III 
hypotension.  Grade III hypotension in temporal association with VLS will be considered also as 
grade III VLS.  VLS resulting in respiratory compromise is considered grade III according to 
CTC 3.0.  Respiratory compromise is defined as symptomatic pulmonary edema requiring 
oxygen or > 10% decrease in oxygen saturation.
Definition of Capillary Leak Syndrome (as stipulated by CTEP) to be utilized after January 1, 
2011:   As specified by CTCAE 4.0, grade II CLS is defined as symptomatic; medical 
intervention is indicated; this protocol further defines that if weight gain is the only feature of 
CLS in a patient, it will be considered a grade II CLS.  If a patient requires more than an hour’s 
worth of hydration of 20 ml/Kg/hour for hypotension, then the patient will be considered to have 
grade III hypotension.  Grade III hypotension in temporal association with CLS will be consider 
also a grade III CLS.  Respiratory compromise in the setting of CLS defined as symptomatic 
pulmonary edema requiring oxygen or > 10% decrease in oxygen saturation will be consider a 
grade III CLS.  Grade III hypotension or grade III CLS is dose limiting.  
4.2.3
Treatment Modifications for Toxicities on Current Cycle
Treatment should not be given on Day 3 or 5 until the laboratory values are reviewed for 
creatinine, AST, ALT, and GGT to determine if treatment modifications are necessary.
Grades I and II Allergic Reaction/hypersensitivity (including drug fever): 

Drug fever only: give acetaminophen every 4-6 hours until resolved (see section 4.8).

Rash (under Allergic reaction in CTC 3.0 to be utilized until December 31, 2010 and 
under Skin and Subcutaneous Tissue Disorder in CTCAE 4.0 to be utilized after January 
1, 2011): follow guidelines in section 4.8 and hold treatment (up to 3 days) until 
symptoms resolve.
Grade II Creatinine: Drug will be held (up to 3 days) until Creatinine is  Grade I.
Grade III Allergic Reaction or Grade II Allergic Reaction with asymptomatic bronchospasm or 
urticaria: Stop treatment. Off treatment (DLT).
Other grade III toxicities except for AST, ALT, GGT, and hematologic: Stop treatment. Cycle 
ends (DLT). 
Grade IV toxicities except for grade 4 hematologic toxicity lasting  5 days: Stop treatment. Off 
treatment (DLT). 
Grade IV hematologic toxicity lasting > 5 days except for alopecia:  Off treatment (DTL).
Grade IV CPK associated with any other DLT or not resolving to < grade II within 
2 
weeks:  Off treatment (DLT).
4.2.4
Treatment Modifications for Toxicities on Previous Cycle
Retreatment: 

All drug-related non-preexisting toxicities should recover to  Grade I prior to 
retreatment except AST, ALT, GGT, must recover to  Grade II.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
19

Patients who entered the study with  Grade II baseline (abnormal) hematological values 
should have recovered to baseline values prior to treatment.
Dose reduction if at least 1 of the following occurred:

Grade III toxicities except for AST, ALT, GGT, fever, and hematologic.

2nd or 3rd dose held >3 days (due to toxicity)

Grade II creatinine
4.2.5
Dose Reductions: 

The 1st dose reduction is to 30 µg/Kg QOD x 3.

The 2nd dose reduction is to 20 µg/Kg QOD x 3.

If a 3rd dose reduction is required, patient is off treatment.

There will be no dose re-escalation.
4.3
PHARMACOKINETIC STUDIES
4.3.1
Analysis of LMB-2 in the plasma
Blood samples will be drawn by the patient care unit registered nurse at the times outlined 
below.  Tubes must be labeled with the patient’s name, medical record number, date of birth, 
date, time drawn, and time related to LMB-2 dose.  Samples drawn from central venous catheters 
may be used as long as it is flushed with 5ml of 0.9% sodium chloride prior to obtaining the 2 
minute post-infusion sample.  Samples of 2 ml of blood will be drawn in a 6 ml sodium heparin 
tube (green top).  Samples of 2 ml blood in serum separator tubes will be drawn with each green 
top tube to allow ELISA assays to be run on stored serum samples. Tubes of blood collected at 
the clinical research center (including both inpatient and outpatient units) should be stored 
upright in the “Kreitman” container in the designated refrigerator for this purpose.  These 
samples will be collected daily Monday through Friday and taken to our lab for analysis. 
4.3.1.1 Days 1 and 5
Inpatients: Pre-dose, 2 minutes after the end of the LMB-2 30-minute infusion, then at 1, 2, 4, 8, 
and 24 hours.
Outpatients: Pre-dose, 2 minutes after the end of the LMB-2 30-minute infusion, then at 1, 2, and 
24 hours.
4.3.1.2 Day 3 
Pre-dose, 2 minutes after the end of the LMB-2 30-minute infusion, then at 24 hours. 
The acceptable error for PK time points is +/- 2 minutes for 2 minute sample; +/- 15 minutes for 
other samples.  PK time points are recommended, and if the actual time point differs, the actual 
time of sample collection should be clearly written on the tube.  


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
20
4.3.2
Analysis of neutralizing antibodies & soluble Tac
At least 2 ml of blood will be drawn in a Serum Separator Tube (SST) prior to starting each 
cycle and between days 17-25 of each cycle. Tubes must be labeled with the patient’s name, 
medical record number, date of birth, date, and time drawn.  Tubes of blood collected at the 
clinical research center should be stored upright in the “Kreitman” container in a designated 
refrigerator.  These samples will be collected daily Monday through Friday and taken to our lab 
for analysis.  Tubes of blood collected outside of the clinical center should be sent by Fed Ex to: 
David Waters, Ph.D. — NIH/SS1 Leidos Biomedical Research, Inc., Building 535, Rm. 428A —  
Frederick, MD  21702 — Phone:  301-846-5831.
The presence of antibodies to LMB-2 is determined by a bioassay performed in a CLIA-certified 
lab in Frederick.  In a 96-well U-bottom plate, serum from patients, or 0.2% human serum 
albumin in PBS (HSA-PBS), is mixed with different concentrations of LMB-2 which are diluted 
in HSA-PBS.  After mixing with serum, the serum-toxin mixtures each contain 90% serum and 
either 0, 40, 200 or 1000 ng/ml of LMB-2.  These mixtures are incubated at 37C for 15 minutes 
and diluted into cell culture media (DMEM+10% FBS) in a U-bottom 96-well plate so that the 
final toxin concentrations are 0, 0.16, 0.8, or 4 ng/ml.  In triplicate, 50 ul aliquots of these diluted 
serum-toxin mixtures are added to 150 ul aliquots of SP2-Tac cells (40,000/well in DMEM+10% 
FBS) in 96-well flat-bottom plates.  After incubating the cells for 16-20 hours at 37C, the cells 
are pulsed for 4-5 hours with {3H}-leucine, harvested, and counted to determine inhibition of 
protein synthesis.  Percent neutralization is calculated by determining the % inhibition of protein 
synthesis of toxin in HSA-PBS, minus the % inhibition in serum, divided by the % inhibition in 
HSA-PBS, multiplied by 100.  For example, if the 1000 ng/ml concentration of LMB-2 + HSA-
PBS caused 75% inhibition of SP2/Tac cells and this concentration in serum caused 50% 
inhibition, the % Neutralization would be 33%.  
4.3.3
Analysis of additional research blood
Other blood samples (≤25 ml per 4 weeks) may be drawn for research purposes. There will be no 
genetic or germ line testing unless the patient is reconsented. 
4.4
ON STUDY EVALUATION
4.4.1
Prior to Each Cycle the following will be done:

Staging will be completed prior to each cycle.  This will include an interim history and 
physical examination with documentation of measurable disease, stage, and performance 
status.  Physical examination should record the diameter, in two planes, of the largest 
palpable nodes in each of the following sites: cervical, axillary, supraclavicular, inguinal, 
and femoral.  Physical examination should record liver and spleen size as determined by 
measurement below the respective costal margin.

FACS analysis of the peripheral blood will be done. 

Laboratory evaluation to be done on or within 3 days prior to day 1 are:  CBC/diff, acute 
care panel, hepatic panel, mineral panel, LDH, PT, PTT, urinalysis, fibrinogen, D-dimer, 
uric acid, amylase, lipase, CK, IgG, IgA, IgM, CRP, ferritin, haptoglobin, Beta-2 
microglobulin and SPEP, 24 hour urine.

ECG, CXR.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
21
4.4.2
Routine Tests done during Each Cycle
4.4.2.1 Days 3, 5 
CBC/diff, acute care panel, hepatic panel, mineral panel, LDH, urinalysis, uric acid, CK.
4.4.2.2 Weeks 2 and 3 
The following labs will be drawn once during weeks 2 and 3: CBC/diff, acute care panel, 
hepatic panel, and albumin. 
4.4.2.3 Follow-Up
For follow-up studies in patients off-treatment, which will not affect eligibility for 
enrollment or retreatment, a non-radioactive neutralization assay may be used, under non-
CLIA conditions.  For additional correlation between the 2 assays, serum samples are 
saved to enable the new assay to be run with historical samples previously tested using 
the radioactive CLIA assay. 
4.4.3
Other tests that will be done at specific times:

Bone marrow biopsy will be done 8 weeks after the patient meets clinical criteria for a 
CR.  A bone marrow biopsy may also be done (but is not required) at the time that 
clinical CR is documented. Research samples at the time of bone marrow aspirate include 
sodium heparin (green top) tubes for MRD analysis and flow cytometry, and an EDTA 
tube for PCR (molecular pathology).  Research blood samples at the time of a bone 
marrow will include flow cytometry, DNA and RNA PaxGene tubes for molecular 
analysis,  and 2 serum separator tubes for neutralizing antibodies and soluble tumor 
markers.  

CT/MRI scan will be done 8 weeks after the patient meets clinical criteria for a CR.  A 
CT/MRI scan may also be done (but is not required) at the time that clinical CR is 
documented. If no lymph nodes are found prior to enrollment, an ultrasound rather than 
CT/MRI may be done at the discretion of the PI.
4.5
COST AND COMPENSATION
4.5.1
Costs
NIH does not bill health insurance companies or participants for any research or related clinical 
care that participants receive at the NIH Clinical Center.  If some tests and procedures performed 
outside the NIH Clinical Center, participants may have to pay for these costs if they are not 
covered by insurance company.  Medicines that are not part of the study treatment will not be 
provided or paid for by the NIH Clinical Center.
4.5.2
Compensation
Participants will not be compensated on this study.
4.5.3
Reimbursement
The NCI will cover the costs of some expenses associated with protocol participation.  Some of 
these costs may be paid directly by the NIH and some may be reimbursed to the 
participant/guardian as appropriate.  The amount and form of these payments are determined by 
the NCI Travel and Lodging Reimbursement Policy.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
22
4.6
TOXICITY CRITERIA  
CTCAE term (AE description) and grade:  The descriptions and grading scales found in the 
revised NCI Common Terminology Criteria for Adverse Events (CTCAE) version 3.0 will be 
utilized until December 31, 2010 for AE reporting.  CTCAE version 4.0 will be utilized 
beginning January 1, 2011.  All appropriate treatment areas should have access to a copy of the 
CTCAE version 4.0.  A copy of the CTCAE version 4.0 can be downloaded from the CTEP web 
site (http://ctep.cancer.gov).
4.7
RETREATMENT
Patients without evidence of progression and without sufficient neutralizing antibodies will be 
eligible to receive retreatment cycles of LMB-2 at the same dose with at least 25 days between 
cycles.  Patients will be tested for neutralizing antibodies prior to each cycle.  Treatment may not 
continue for beyond two cycles for a complete response (CR), beyond four cycles for CR with 
minimal residual disease (MRD), or beyond six cycles for a partial response (PR) or stable 
disease (SD) without prior FDA approval.
4.8
SUPPORTIVE CARE

Allergic reaction will be treated acutely with antihistamines (including diphenhydramine, 
hydroxyzine, & ranitidine), fluids, bronchodilators, and/or epinephrine.

Nausea and Vomiting: Patients who develop nausea will be treated with a serotonin 5-HT 
receptor inhibitor for at least 24 hours after their last episode of nausea.  Other 
antiemetics such as prochlorperazine, metoclopramide, or lorazepam may be used in 
addition if necessary.

Myalgias: Patients who develop myalgias may be given acetaminophen 650 to 1000 mg 
every 6 hours until 24 hours after completing the last dose of LMB-2.  It may then be 
given as needed. Patients may receive NSAIDs or opioid analgesics if acetaminophen is 
inadequate.

Vascular leak syndrome/Capillary leak syndrome: supportive care may include fluid and 
electrolyte management, diuresis, albumin, and cardiovascular support. 

Hypotension: Patients will be encouraged to increase oral fluid intake.  In addition, for an 
orthostatic SBP change of >20 mm Hg and an absolute SBP of <100 mm Hg, an IVF 
bolus may be given as deemed clinically appropriate.  Refractory hypotension may 
require treatment in the intensive care unit with pressors.

Fever: Patients who develop temperatures >38.0 C may receive scheduled 
acetaminophen 650 to 1000 mg every 6 hours until 24 hours after completing the last 
dose of LMB-2. It may then be given as needed.

Thrombocytopenia should be treated conservatively. In the absence of bleeding or a 
planned invasive procedure, platelet transfusions should only be given for a platelet count 
below 10,000. If invasive procedures are planned or the patient develops bleeding, 
platelet transfusions should be administered in accordance with standard of practice, 
usually maintaining a platelet count > 50,000/mm3.

Symptomatic anemia should be treated with appropriate red blood cell support. 
Transfusion is recommended if the hemoglobin falls below 8g/dL.  Recombinant 
erythropoietin may be also be used.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
23

Febrile Neutropenia is a life-threatening complication requiring hospitalization and 
urgent broad-spectrum antibiotics. Hematopoietic growth factors may be used if 
clinically indicated.  Such cases will be evaluated individually to determine the toxicity 
grade.  Neutropenia due to LMB-2 is not expected.

Central venous access devices such as a temporary internal jugular or subclavian lines, 
PICC lines, semi-permanent HICKMAN, Groshong catheters, or medi-port implanted 
devices can all be used in this study.  All devices will have nursing supervision and 
include patient self-care instruction.

Nutritional assessment and psychological support: Refractory neoplasms are commonly 
complicated by malnutrition. Patients with weight loss or evidence of wasting syndrome 
should have a nutritional consult.  When necessary, social Work will be proactively 
involved with these patients’ biopsychosocial well-being.
4.9
CRITERIA FOR REMOVAL FROM PROTOCOL THERAPY AND OFF STUDY CRITERIA
Prior to removal from study, effort must be made to have all subjects complete a safety visit 
approximately 30 days following the last dose of study therapy.
4.9.1
Criteria for removal from protocol therapy

Progression of disease during active treatment on study protocol.

Grade III Allergic Reaction and grade II urticaria despite premedication.

More than 2 dose reductions are required.

Patient non-compliance or voluntary withdrawal.

Patient’s serum neutralizes > 75% of the activity of 1 µg/mL of LMB-2.

Grade IV toxicity other than hematologic and CPK. 

Grade IV hematologic toxicity lasting > 5 days. 

Grade IV CPK associated with any other DLT or not resolving to < grade II within 2 
weeks.
4.10 POST TREATMENT EVALUATION (OFF TREATMENT BUT ON STUDY) 
Patients will be taken off treatment when they meet off treatment criteria.  Patients who are off 
treatment due to toxicity will be followed until resolution of their side effects. On- or off-
treatment patients who have not had progressive disease will be followed every 3-12 months 
until they have progressive disease (see Section 12, Study Calendar).  Patients who have 
progressive disease may also need follow-up for other endpoints, including neutralizing 
antibodies (see Section 12, Study Calendar) .  Follow-up assessments will be conducted per PI 
discretion and may be obtained from clinic visits either at NIH or elsewhere.
4.11 OFF-STUDY CRITERIA 

Screen failure

Patient begins another LMB trial which allows data collection 

Unwillingness to continue follow-up or provide follow-up data.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
24
4.12 LOST TO FOLLOW-UP
A participant will be considered lost to follow-up if he or she fails to return for 3 consecutive 
scheduled visits and is unable to be contacted by the study site staff. 
The following actions must be taken if a participant fails to return to the clinic for a required 
study visit:

The site will attempt to contact the participant and reschedule the missed visit within 2 
weeks and counsel the participant on the importance of maintaining the assigned visit 
schedule and ascertain if the participant wishes to and/or should continue in the study.

Before a participant is deemed lost to follow-up, the investigator or designee will make 
every effort to regain contact with the participant (where possible, 3 telephone calls and, 
if necessary, a certified letter to the participant’s last known mailing address or local 
equivalent methods). These contact attempts should be documented in the participant’s 
medical record or study file. 

Should the participant continue to be unreachable, he or she will be considered to have 
withdrawn from the study with a primary reason of lost to follow-up.
5
HUMAN SUBJECTS PROTECTIONS
5.1
RATIONALE FOR SUBJECT SELECTION
Selection is based on gender, ethnic background or race.
Subjects from both genders and all racial/ethnic groups are eligible for this study if they meet the 
eligibility criteria. To date, there is no information that suggests that differences in drug 
metabolism or disease response would be expected in any one patient group.  Efforts will be 
made to extend accrual to a representative population, but in this preliminary study, a balance 
must be struck between patient safety considerations and limitations on the number of patients 
exposed to a potentially toxic treatment on the one hand and the need to explore gender and 
ethnic aspects of clinical research on the other.  If differences in the outcome which correlate 
with gender or ethnic identity are noted, accrual may be expanded or a follow-up study may be 
written to investigate these differences.
5.1.1.1 Justification for Exclusions
Patients with hepatitis C positivity are excluded from this trial because of the concern that LMB-
2 may worsen hepatitis C.  Patients with hepatitis B are not excluded if on Lamivudine, which is 
used to prevent reactivation/worsening of hepatitis B with chemotherapy and other 
immunosuppressive treatments.  Patients that are pregnant or breast-feeding will be excluded 
from this trial because the effect of LMB-2 on the developing fetus or the nursing infant is 
unknown and potentially harmful.
5.2
PARTICIPATION OF CHILDREN
Only patients 18 years of age or older will be enrolled on this study, since the safety of this agent 
has not been previously defined in a pediatric population.
5.3
PARTICIPATION OF SUBJECTS UNABLE TO CONSENT 
Adults unable to give consent are excluded from enrolling in the protocol.  However, re-consent 
may be necessary and there is a possibility, though unlikely, that subjects could become 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
25
decisionally impaired. For this reason and because there is a prospect of direct benefit from 
research participation (section 5.5), all subjects will be offered the opportunity to fill in their 
wishes for research and care, and assign a substitute decision maker on the “NIH Advance 
Directive for Health Care and Medical Research Participation” form so that another person can 
make decisions about their medical care in the event that they become incapacitated or 
cognitively impaired during the course of the study. Note: The PI or AI will contact the NIH 
Ability to Consent Assessment Team(ACAT) for evaluation to assess ongoing capacity of the 
subjects and to identify an LAR, as needed.
Please see Section 5.6.1 for consent procedure.
5.4
EVALUATION OF BENEFITS AND RISKS/DISCOMFORTS
5.4.1
Potential benefits to subjects expected from the trial
Patients will receive evaluation and treatment of their tumor at the National Cancer Institute’s 
Clinical Center.  This protocol may or may not benefit an individual, but the results may help the 
investigators learn more about the disease and develop new treatments for patients with this 
disease.   Benefit cannot be promised nor can the chance of benefit be accurately predicted.  This 
research treatment is unlikely to be curative but may offer temporary control of the disease.
5.4.2
Alternative approaches or treatments
Patients will be consented verbally and in writing regarding the risks and benefits of this trial, the 
treatment requirements, and alternative approaches to entering on this trial.
5.4.3
Procedures for protecting against or minimizing any potential risks
All care will be taken to minimize side effects, but they can be unpredictable in nature and 
severity.  This study may involve risks to patients which are currently unforeseeable.  Patients 
will be examined and evaluated prior to enrollment and prior to each cycle.  The Clinical Center 
staff will observe all patients during the drug administration.  All evaluations to monitor the 
treatment of patients will be recorded in the patient chart.  Patients are required to have a local 
physician to improve long-term care and to monitor for complications.  They will have blood 
draws at home to monitor side effects.  If patients suffer any physical injury as a result of the 
participation in this study, immediate medical treatment is available at the Clinical Center, 
National Cancer Institute, Bethesda, Maryland.  Although no compensation is available, any 
injury will be evaluated and treated in keeping with the benefits or care to which patients are 
entitled under applicable regulations.
5.4.4
Identified Risks to Study Procedures
5.4.4.1 Blood Sampling
Side effects of blood draws include pain and bruising, lightheadedness, and rarely, fainting. 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
26
5.4.4.2 Imaging
In addition to the radiation risks discussed below, CT scans may include the risks of an allergic 
reaction to the contrast. Participants might experience hives, itching, headache, difficulty 
breathing, increased heartrate and swelling.
5.4.4.3 MR Imaging
The risks of MR imaging are relatively small.
The US Food and Drug Administration has issued warnings that administration of gadolinium 
(updated September 9, 2010), the MRI contrast imaging agent used in this protocol, has been 
associated with development of a disease called nephrogenic systemic fibrosis (NSF). The 
syndrome is rare (approximately 600 cases reported worldwide as of September 2010, out of 
several million administrations of gadolinium), but disabling and in some cases, fatal. All cases 
to date have occurred in patients with severe renal disease, including patients on dialysis. NSF 
has been nearly eradicated secondary to careful screening of renal function and avoiding use of 
gadolinium in patients with eGFR <30 ml/min/1.73 BSA. Even in patients with end stage renal 
disease, there have been only rare occurrences of NSF because of precautions taken to use more 
stable contrast agents at lower doses. This protocol excludes patients with severe renal 
insufficiency (eGFR <30 ml/min/1.73 BSA). The FDA has issued warnings in 2017 and 2018 
that some gadolinium may be retained in the brain, bone and skin although health risks of 
accumulation have not been reported to date. In accordance with the FDA Drug Safety 
Communication of 05/16/2018, the Medication Guide for gadobutrol (or other macrocyclic 
gadolinium contrast agent if applicable) will be made available to all subjects with scans that will 
involve gadolinium-based contrast agent administration.
5.4.5
Risks from Radiation Exposure
On this study, participants will receive up to 4 CT scans/year The total radiation dose for 
research purposes will be approximately 5.2 rem. The risk of getting cancer from the radiation 
exposure in this study is 0.5% and of getting a fatal cancer is 0.3%.
5.4.6
Provisions for monitoring data collection to ensure safety of subjects
As information is gathered from this trial, clinical results will be shared with patients while 
maintaining patient confidentiality. Laboratory and clinical data will be frequently gathered and 
any new significant findings found during the course of the research, which may affect a 
patient’s willingness to participate further, will be explained.  Moreover, in all publications and 
presentations resulting from this trial, patients’ anonymity will be protected to the maximum 
extent possible.  Authorized personnel from the National Cancer Institute (NCI) and Food and 
Drug Administration (FDA) may have access to research files in order to verify that patients’ 
rights have been safeguarded.  In addition, patient names will be given to the Central 
Registration to register and verify patients’ eligibility. 
Primary and final analyzed data will have identifiers so that research data can be attributed to an 
individual human subject participant.
End of study procedures: Data will be stored according to HHS, FDA regulations and NIH 
Intramural Records Retention Schedule as applicable. 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
27
5.5
RISKS/BENEFITS ANALYSIS
Patients enrolled on this study will be those with a disease that is considered incurable.  They 
will generally have a poor prognosis and have no standard treatment options known to 
significantly improve survival.  Thus, patients may experience significant treatment related 
morbidity, and/or have progressive complications of their disease.  Although LMB-2 is an 
experimental new immunotoxin, another drug with a similar mechanism of action and similar 
toxicities was recently approved for CTCL.  In addition, we have seen responses to LMB-2 in 
patients with CLL and HCL with acceptable toxicities.  Consequently, we believe that patients 
participating in this trial will have an acceptable benefit/risk ratio.
5.6
CONSENT PROCESS AND DOCUMENTATION
The informed consent document will be provided as a physical or electronic document to the 
participant or consent designee(s) as applicable for review prior to consenting.  A designated 
study investigator will carefully explain the procedures and tests involved in this study, and the 
associated risks, discomforts and benefits. In order to minimize potential coercion, as much time 
as is needed to review the document will be given, including an opportunity to discuss it with 
friends, family members and/or other advisors, and to ask questions of any designated study 
investigator. A signed informed consent document will be obtained prior to entry onto the study.
The initial consent process as well as re-consent, when required, may take place in person or 
remotely (e.g., via telephone or other NIH approved remote platforms used in compliance with 
policy, including HRPP Policy 303) per discretion of the designated study investigator and with 
the agreement of the participant/consent designee(s).  Whether in person or remote, the privacy 
of the subject will be maintained. Consenting investigators (and participant/consent designee, 
when in person) will be located in a private area (e.g., clinic consult room). When consent is 
conducted remotely, the participant/consent designee will be informed of the private nature of 
the discussion and will be encouraged to relocate to a more private setting if needed.
Consent will be documented with required signatures on the physical document (which includes 
the printout of an electronic document sent to participant) or as described below, with a manual 
(non-electronic) signature on the electronic document. When required, witness signature will be 
obtained similarly as described for the investigator and participant.
Manual (non-electronic) signature on electronic document:
When a manual signature on an electronic document is used for the documentation of consent at 
the NIH Clinical Center, this study will use the following to obtain the required signatures:

Adobe platform (which is not 21 CFR Part 11 compliant); or, 

iMedConsent platform (which is 21 CFR Part 11 compliant)
During the consent process, participants and investigators will view individual copies of the 
approved consent document on screens at their respective locations (if remote consent); the same 
screen may be used when in the same location but is not required.  
Both the investigator and the participant will sign the document using a finger, stylus or mouse. 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
28
Note: Refer to the CCR SOP PM-2, Obtaining and Documenting the Informed Consent Process 
for additional information (e.g., verification of participant identity when obtaining consent 
remotely) found at: 
https://ccrod.cancer.gov/confluence/pages/viewpage.action?pageId=73203825.
5.6.1
Consent Process for Adults Who Lack Capacity to Consent to Research Participation
For participants addressed in Section 5.3, an LAR will be identified consistent with Policy 403 
and informed consent obtained from the LAR, as described in Section 5.6.
5.6.2
Consent to obtain samples for eligibility
The patient must give informed consent even before protocol enrollment, at the time that samples 
are taken to test eligibility.  A separate informed consent is given to the patient clarifying what 
tests will be done and stating that no germline testing is to be done.  This eligibility informed 
consent also states that the patient may eventually not be considered a candidate for the study.  
The eligibility consent may be signed by the patient prior to arrival at NIH, but the actual 
treatment (standard) consent must be signed in person prior to protocol enrollment. 
5.7
SAMPLE STORAGE, TRACKING AND DISPOSITION
5.7.1.1 Description of data/specimens:  Blood, bone marrow, lymph node, skin, and other tumor 
samples.
5.7.1.2 Research being conducted:  Samples to be saved for additional tests:
Neutralizing antibodies: Antibodies a patient might make which block the effect of 
certain recombinant immunotoxins like LMB-2. Requires about 6 ml of blood.
Flow cytometry assays to quantify tumor markers on the malignant cells. Requires about 
3 ml of blood.
Bone marrow biopsy samples, whether they obtained at NIH or elsewhere, and whether 
the bone marrow test has already been done or not yet done.
Cytotoxicity assays.  Leukemia cells from the blood, bone marrow, or other tissues may 
be tested with LMB-2 and related drugs to determine if the malignant cells can be killed 
outside the body. Requires 15-45 ml of blood.
Soluble CD25, CD22, and other tumor markers:  To estimate the amount of cancer cells 
in the body by measuring proteins which fall off cancer cells and go into the blood. 
Requires about 6 ml of blood.
HLA typing to better understand the immune system in patients with hairy cell leukemia.  
Requires about 6 ml of blood.
PAX-gene tube: To obtain RNA or DNA to study the mechanism of how leukemia cells 
form, and to detect very low levels of leukemia cells in patients.  Requires about 3 ml of 
blood.
RNA samples can also be used, in an assay called micro-arrays, to study why some 
patients may not respond as well as others to recombinant immunotoxins like LMB-2.  
Taken with PaxGene tube.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
29
Samples of blood to study how hemolytic uremic syndrome (HUS), a major toxicity of a 
recombinant immunotoxin called BL22, which is similar to LMB-2, occurs and might be 
prevented.  Requires about 3 ml of blood.
DNA samples to look for abnormalities which might make a patient more susceptible to 
HUS. Requires about 3 ml of blood.
Assays which could have an impact on both patients and their children, including studies 
of genetic cancer risk, will not be done.
Samples to determine levels of immunotoxin in blood, urine, and other tissues.
5.7.1.3 Timeframe and location of storage:  Samples will be stored and cataloged longer than a 
year, in alarmed freezers at our Leidos Biomedical Research, Inc. contract lab in 
Frederick, MD, where neutralizing antibodies and PK samples are tested. Portions of 
samples which are stored at Leiodos Biomedical Research, Inc. in Frederick may also 
be stored and tested in the LMB lab (Building 37) for longer than a year providing there 
is sample remaining after studies are done.  All samples will be stored with unique 
patient numbers and without personal identifiers.   After closure of the protocol, the 
samples will either be destroyed or their storage and use will be governed by a 
subsequent protocol.  Samples at Leiodos Biomedical Research, Inc. in Frederick will 
be tracked in a secure electronic database and the PI will report lost or destroyed 
samples per the requirements of Section 8.2. 
6
DOSING DELAYS/DOSE MODIFICATION
6.1
NO DLT  
Continue treatment or retreatment at same dosage.
6.2
DLT  
This also applies to patients undergoing retreatment cycles.
6.2.1
Off Treatment for DLT from:

Grade III Allergic Reaction and grade II urticaria despite premedication.

Grade IV toxicity other than hematologic and CPK. 

Grade IV hematologic toxicity lasting > 5 days. 

Grade IV CPK associated with any other DLT or not resolving to < grade II within 
2 weeks. 
6.2.2
Requiring dose reduction if retreating after DLT from:

Grade III toxicities except for AST, ALT, GGT, and hematologic.

Grade IV CPK not associated with any other DLT and resolving to < grade II within 
2 weeks. 
6.3
DOSE DELAY  
Dosing may be delayed to evaluate patients without DLT, including those with infections, or 
with complications of disease or central lines.  If LMB-2 therapy is suspended longer than 72 
hours during a cycle for toxicity, therapy will not be resumed during that cycle.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
30
7
ADVERSE EVENTS:  LIST AND REPORTING REQUIREMENTS (SPONSOR)
Adverse event (AE) monitoring and reporting is a routine part of every clinical trial.  The 
following list of AEs (Section 7.1) and the characteristics of an observed AE (Section 7.2) will 
determine whether the event requires expedited reporting (via CTEP-AERS) in addition to 
routine reporting.  
7.1
COMPREHENSIVE ADVERSE EVENTS AND POTENTIAL RISKS LIST(S) (CAEPRS)
The Comprehensive Adverse Event and Potential Risks list (CAEPR) provides a single list of 
reported and/or potential adverse events (AE) associated with an agent using a uniform 
presentation of events by body system. In addition to the comprehensive list, a subset, the 
Specific Protocol Exceptions to Expedited Reporting (SPEER), appears in a separate column and 
is identified with bold and italicized text. This subset of AEs (SPEER) is a list of events that are 
protocol specific exceptions to expedited reporting to NCI via CTEP-AERS (except as noted 
below). Refer to the 'CTEP, NCI Guidelines: Adverse Event Reporting Requirements'  
http://ctep.cancer.gov/protocolDevelopment/adverse_effects.htm for further clarification. The 
CAEPR does not provide frequency data; refer to the Investigator's Brochure for this 
information. Below is the CAEPR for LMB-2 (Anti-Tac[Fv]-PE-38).
NOTE: Report AEs on the SPEER ONLY IF they exceed the grade noted in parentheses next to 
the AE in the SPEER. If this CAEPR is part of a combination protocol using multiple 
investigational agents and has an AE listed on different SPEERs, use the lower of the grades to 
determine if expedited reporting is required.
7.1.1
Comprehensive Adverse Events and Potential Risks List (CAEPR) for LMB-2 
Immunotoxin (Anti-Tac[Fv]-PE-38, NSC 676422)
Version 1.3, July 26, 20131
Adverse Events with 
Possible Relationship to LMB-
2 (Anti-Tac[Fv]-PE-38)
(CTCAE 4.0 Term)
Specific Protocol Exceptions 
to Expedited 
Reporting (SPEER)
BLOOD AND LYMPHATIC SYSTEM DISORDERS
Anemia
CARDIAC DISORDERS
Left ventricular systolic dysfunction
Left ventricular systolic dysfunction 
(Gr 2)
Pericardial effusion
Pericardial effusion (Gr 2)
Sinus tachycardia
GASTROINTESTINAL DISORDERS
Abdominal distension
Diarrhea
Nausea
Nausea (Gr 2)
Vomiting
Vomiting (Gr 2)
GENERAL DISORDERS AND ADMINISTRATION 
Edema face
Edema face (Gr 2)
Edema limbs
Edema limbs (Gr 2)
Fatigue
Fatigue (Gr 2)
Fever
Fever (Gr 2)
IMMUNE SYSTEM DISORDERS
Allergic reaction
Allergic reaction (Gr 2)


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
31
Adverse Events with 
Possible Relationship to LMB-
2 (Anti-Tac[Fv]-PE-38)
(CTCAE 4.0 Term)
Specific Protocol Exceptions 
to Expedited 
Reporting (SPEER)
INVESTIGATIONS
Alanine aminotransferase increased
Alanine aminotransferase increased 
(Gr 2)
Alkaline phosphatase increased
Aspartate aminotransferase increased
Aspartate aminotransferase 
increased (Gr 2)
CPK increased
Creatinine increased
Creatinine increased (Gr 2)
GGT increased
Platelet count decreased
Platelet count decreased (Gr 2)
Weight gain
Weight gain (Gr 2)
METABOLISM AND NUTRITION DISORDERS
Anorexia
Hypoalbuminemia
Hypoalbuminemia (Gr 2)
MUSCULOSKELETAL AND CONNECTIVE TISSUE 
Back pain
Chest wall pain
Myalgia
Myalgia (Gr 2)
NERVOUS SYSTEM DISORDERS
Dizziness
Headache
Headache (Gr 2)
RENAL AND URINARY DISORDERS
Hematuria
Hematuria (Gr 2)
Proteinuria
Proteinuria (Gr 2)
RESPIRATORY, THORACIC AND MEDIASTINAL 
Dyspnea
Pleural effusion
VASCULAR DISORDERS
Capillary leak syndrome
Capillary leak syndrome (Gr 2)
Hypotension
Hypotension (Gr 2)
1This table will be updated as the toxicity profile of the agent is revised.  Updates will be 
distributed to all Principal Investigators at the time of revision.  The current version can be 
obtained by contacting PIO@CTEP.NCI.NIH.GOV.  Your name, the name of the investigator, 
the protocol and the agent should be included in the e-mail.
2 Infection includes all 75 sites of infection under the INFECTIONS AND INFESTATIONS 
SOC.
3Muscle weakness includes Generalized muscle weakness, Muscle weakness left-sided, Muscle 
weakness lower limb, Muscle weakness right-sided, Muscle weakness trunk, and Muscle 
weakness upper limb under the MUSCULOSKELETAL AND CONNECTIVE TISSUE 
DISORDERS SOC.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
32
Also reported on LMB-2 (Anti-Tac[Fv]-PE-38) trials but with the relationship to LMB-2 
(Anti-Tac[Fv]-PE-38) still undetermined:
CARDIAC DISORDERS - Acute coronary syndrome; Myocardial infarction; Paroxysmal atrial 
tachycardia; Restrictive cardiomyopathy; Supraventricular tachycardia
EYE DISORDERS - Blurred vision; Eye pain
GASTROINTESTINAL DISORDERS - Dyspepsia
GENERAL DISORDERS AND ADMINISTRATION SITE CONDITIONS - Chills; Non-
cardiac chest pain
IMMUNE SYSTEM DISORDERS - Anaphylaxis
INFECTIONS AND INFESTATIONS – Infection2
INJURY, POISONING AND PROCEDURAL COMPLICATIONS - Fall
INVESTIGATIONS - Blood bilirubin increased; Cardiac troponin I increased; Fibrinogen 
decreased; Weight loss
METABOLISM AND NUTRITION DISORDERS - Hypokalemia
MUSCULOSKELETAL AND CONNECTIVE TISSUE DISORDERS - Bone pain; Muscle 
weakness3; Musculoskeletal and connective tissue disorder -  Other (acute rhabdomyolysis); 
Neck pain; Pain in extremity
NERVOUS SYSTEM DISORDERS - Dysgeusia; Presyncope; Syncope; Vasovagal reaction
RESPIRATORY, THORACIC AND MEDIASTINAL DISORDERS - Allergic rhinitis; 
Pneumonitis
SKIN AND SUBCUTANEOUS TISSUE DISORDERS - Hyperhidrosis; Palmar-plantar 
erythrodysesthesia syndrome; Pruritus; Rash maculo-papular
VASCULAR DISORDERS - Hypertension
Animal Data: The following toxicities have been observed in animal studies with LMB-2 
(Anti-Tac[Fv]-PE-38):
leukocytosis
Note: LMB-2 (Anti-Tac[Fv]-PE-38) in combination with other agents could cause an 
exacerbation of any adverse event currently known to be caused by the other agent, or the 
combination may result in events never previously associated with either agent.
7.2
ADVERSE EVENT CHARACTERISTICS 

CTCAE term (AE description) and grade:  The descriptions and grading scales 
found in the revised NCI Common Terminology Criteria for Adverse Events 
(CTCAE) version 4.0 will be utilized for AE reporting.  All appropriate treatment 
areas should have access to a copy of the CTCAE version 4.0.  A copy of the 
CTCAE version 4.0 can be downloaded from the CTEP web site 
http://ctep.cancer.gov/protocolDevelopment/electronic_applications/ctc.htm.  

For expedited reporting purposes only:  
o
AEs for the agent that are bold and italicized in the CAEPR (i.e., those 
listed in the SPEER column, Section 7.1.1) should be reported through 
CTEP-AERS only if the grade is above the grade provided in the 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
33
SPEER.
o
Other AEs for the protocol that do not require expedited reporting 
are outlined in section Error! Reference source not found.

Attribution of the AE:
-
Definite – The AE is clearly related to the study treatment.
-
Probable – The AE is likely related to the study treatment.
-
Possible – The AE may be related to the study treatment.
-
Unlikely – The AE is doubtfully related to the study treatment.
-
Unrelated – The AE is clearly NOT related to the study treatment.
7.3
EXPEDITED ADVERSE EVENT REPORTING
7.3.1
Expedited AE reporting for this study must use CTEP-AERS (CTEP Adverse Event 
Reporting System), accessed via the CTEP Web site (http://ctep.cancer.gov).  The 
reporting procedures to be followed are presented in the “NCI Guidelines for 
Investigators: Adverse Event Reporting Requirements for DCTD (CTEP and CIP) and 
DCP INDs and IDEs” which can be downloaded from the CTEP Web site 
(http://ctep.cancer.gov).  These requirements are briefly outlined in the tables below 
(Section 7.3.2).
In the rare occurrence when Internet connectivity is lost, a 24-hour notification is to be 
made to CTEP by telephone at 301-897-7497.  Once Internet connectivity is restored, the 
24-hour notification phoned in must be entered electronically into CTEP-AERS by the 
original submitter at the site.
7.3.2
Expedited Reporting Guidelines 
Note:  A death on study requires both routine and expedited reporting regardless of 
causality, unless as noted below.  Attribution to treatment or other cause must be provided.
Use the NCI protocol number and the protocol-specific patient ID assigned during trial 
registration on all reports.
Phase 2 and 3 Trials Utilizing an Agent under a CTEP IND:  Reporting Requirements for 
Adverse Events That Occur Within 30 Days1 of the Last Dose of the Investigational Agent
Grade 1
Grade 
2
Grade 
2
Grade 3
Grade 3
Grades
4 & 52
Grades
4 & 52
Unexpect
ed 
and 
Expected
Unex-
pected Expect
ed
Unexpected
Expected
Unex-
pected 
Expected


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
34
with 
Hospita
li-zation
without 
Hospital
i- zation
with 
Hospita
li-
zation
without 
Hospital
i-
zation
Unrelat
ed
Unlikel
y
Not
Required
Not
Requir
ed
Not
Requir
ed
10 
Calenda
r
Days
Not
Require
d
10 
Calend
ar
Days
Not
Require
d
10
Calendar
Days
10
Calendar
Days
Possibl
e
Probabl
e
Definit
e
Not
Required
10 
Calend
ar
Days
Not
Requir
ed
10 
Calenda
r
Days
10 
Calenda
r
Days
10 
Calend
ar
Days
Not
Require
d
24-Hour;
5 
Calendar
Days
10
Calendar
Days
1
Adverse events with attribution of possible, probable, or definite that occur greater 
than 30 days after the last dose of treatment with an agent under a CTEP IND require 
reporting as follows:
CTEP-AERS 24-hour notification followed by complete report within 5 calendar days for:
·
Grade 4 and Grade 5 unexpected events 
CTEP-AERS 10 calendar day report:
·
Grade 3 unexpected events with hospitalization or prolongation of hospitalization
·
Grade 5 expected events
2
Although an CTEP-AERS 24-hour notification is not required for death clearly 
related to progressive disease, a full report is required as outlined in the table.
December 15, 2004
7.4
DATA COLLECTION AND EVALUATION  
All observed or volunteered adverse events, regardless of treatment group or suspected causal 
relationship to study drug, will be recorded into a 21 CFR Part 11-compliant data capture system 
. Adverse events must be reported in a consistent manner for all patients on study, based on the 
following rules:  Adverse events will be identified and graded using the NCI CTCAE version 3 
until December 31, 2010.  CTCAE version 4.0 will be utilized beginning January 1, 2011. Next, 
it will be assessed if the adverse event is related to the medical treatment (attribution). If so, it 
will be determined whether the adverse event is expected or unexpected (see Investigator’s 
Brochure and section 7.4.1). If, in the judgment of the PI, the adverse event is not constant but 
fluctuates (or stutters) or changes grade during a period of time, it may be reported as one event 
with the grade being the maximum grade reached, and the resolved date being the date it returns 
to baseline grade.  Clinical judgment of the PI must be used to assess the baseline grade of 
adverse events.  The laboratory value immediately before beginning drug is usually used to 
determine grades of baseline laboratory AEs, but laboratory values from the prior 90 days may 
be used if clinically relevant.  All calcium values will be corrected for albumin.  To determine 
the corrected calcium in mmol/L, subtract the albumin in g/dL from 4.0, multiply the result by 
0.2, then add the product to the measured calcium in mmol/L.  


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
35
7.4.1
Expected Adverse Events
7.4.1.1 Grade 4: none
7.4.1.2 Grade 3: AST, SGOT, ALT, SGPT, GGT, Albumin, serum-low (hypoalbuminemia), 
platelets.
7.4.1.3 Grade 2: 

Blood Bone Marrow: platelets, hemoglobin, Neutrophils/granulocytes (ANC/AGC), 
Leukocytes (total WBC), lymphopenia.

Cardiovascular: Acute Vascular Leak syndrome/Capillary Leak syndrome (CTEP 
defined section 4.2.2), edema, hypotension, pericardial effusion/pericarditis, PTT, 
INR (International Normalized Ratio of prothrombin time).

Constitutional: fatigue, fever, weight gain.

GI: nausea, vomiting, diarrhea.

Hepatic: AST, SGOT, ALT, SGPT, GGT, Albumin, serum-low (hypoalbuminemia), 
alkaline phosphatase.

Metabolic/Laboratory: bicarbonate, CPK, Calcium, serum-low (hypocalcemia), 
Potassium, serum-low (hypokalemia), Magnesium, serum-low (hypomagnesemia), 
Sodium, serum-low (hyponatremia), Phosphate, serum-low (hypophosphatemia).

Musculoskeletal: muscle weakness.

Pain: myalgia.

Renal: creatinine, proteinuria.
All Adverse Events must be reported in routine study data submissions.  AEs reported through 
CTEP-AERS must also be reported in routine study data submissions.
8
NIH REPORTING REQUIREMENTS / DATA AND SAFETY MONITORING PLAN
8.1
DEFINITIONS
Please refer to definitions provided in Policy 801: Reporting Research Events found at: 
https://irbo.nih.gov/confluence/pages/viewpage.action?pageId=36241835#Policies&Guidance-
800Series-ComplianceandResearchEventReportingRequirements..
8.2
OHSRP OFFICE OF COMPLIANCE AND TRAINING / IRB REPORTING
8.2.1
Expedited Reporting 
Please refer to the reporting requirements in Policy 801: Reporting Research Events and Policy 
802 Non-Compliance Human Subjects Research found at: 
https://irbo.nih.gov/confluence/pages/viewpage.action?pageId=36241835#Policies&Guidance-
800Series-ComplianceandResearchEventReportingRequirements.. Note: Only IND Safety 
Reports that meet the definition of an unanticipated problem will need to be reported per these 
policies.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
36
8.2.2
IRB Requirements for PI Reporting at Continuing Review
Please refer to the reporting requirements in Policy 801: Reporting Research Events found at: 
https://irbo.nih.gov/confluence/pages/viewpage.action?pageId=36241835#Policies&Guidance-
800Series-ComplianceandResearchEventReportingRequirements.. 
8.3
NCI CLINICAL DIRECTOR REPORTING
Problems expeditiously reviewed by the OHSRP in the NIH eIRB system will also be reported to 
the NCI Clinical Director/designee; therefore, a separate submission for these reports is not 
necessary.
In addition to those reports, all deaths that occur within 30 days after receiving a research 
intervention should be reported via email unless they are due to progressive disease.  
To report these deaths, please send an email describing the circumstances of the death to  
NCICCRQA@mail.nih.gov within one business day of learning of the death.
8.4
NIH REQUIRED DATA AND SAFETY MONITORING PLAN
The clinical research team will meet on a regular basis (at least weekly) when participants are 
being actively treated on the trial to discuss each participant. Decisions about dose level 
enrollment and dose escalation if applicable will be made based on the toxicity data from prior 
participants. 
All data will be collected in a timely manner and reviewed by the principal investigator or a lead 
associate investigator. Events meeting requirements for expedited reporting as described in 
Section 8.2.1 will be submitted within the appropriate timelines.  
The principal investigator will review adverse event and response data on each participant to 
ensure safety and data accuracy. The principal investigator will personally conduct or supervise 
the investigation and provide appropriate delegation of responsibilities to other members of the 
research staff.
9
REGULATORY AND OPERATIONAL CONSIDERATIONS
9.1
STUDY DISCONTINUATION AND CLOSURE
This study may be temporarily suspended or prematurely terminated if there is sufficient 
reasonable cause.  Written notification, documenting the reason for study suspension or 
termination, will be provided by the suspending or terminating party to study participants, 
investigator, the Investigational New Drug (IND) sponsor and regulatory authorities.  If the study 
is prematurely terminated or suspended, the Principal Investigator (PI) will promptly inform 
study participants, and the Institutional Review Board (IRB), and will provide the reason(s) for 
the termination or suspension.  Study participants will be contacted, as applicable, and be 
informed of changes to study visit schedule.
 Circumstances that may warrant termination or suspension include, but are not limited to:

Determination of unexpected, significant, or unacceptable risk to participants

Demonstration of efficacy that would warrant stopping   


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
37

Insufficient compliance to protocol requirements

Data that are not sufficiently complete and/or evaluable

Determination that the primary endpoint has been met

Determination of futility
Study may resume once concerns about safety, protocol compliance, and data quality are 
addressed, and satisfy the IRB and as applicable, Food and Drug Administration (FDA).
9.2
QUALITY ASSURANCE AND QUALITY CONTROL
The clinical site will perform internal quality management of study conduct, data and biological 
specimen collection, documentation and completion.  An individualized quality management 
plan will be developed to describe a site’s quality management.
Quality control (QC) procedures will be implemented beginning with the data entry system and 
data QC checks that will be run on the database will be generated. Any missing data or data 
anomalies will be communicated to the site for clarification/resolution.
Following written Standard Operating Procedures (SOPs), the monitors will verify that the 
clinical trial is conducted and data are generated and biological specimens are collected, 
documented (recorded), and reported in compliance with the protocol, International Conference 
on Harmonisation Good Clinical Practice (ICH GCP), and applicable regulatory requirements 
(e.g., Good Laboratory Practices (GLP), Good Manufacturing Practices (GMP)). 
The investigational site will provide direct access to all trial related sites, source data/documents, 
and reports for the purpose of monitoring and auditing by the sponsor, and inspection by local 
and regulatory authorities.
9.3
CONFLICT OF INTEREST POLICY
The independence of this study from any actual or perceived influence, such as by the 
pharmaceutical industry, is critical.  Therefore, any actual conflict of interest of persons who 
have a role in the design, conduct, analysis, publication, or any aspect of this trial will be 
disclosed and managed. Furthermore, persons who have a perceived conflict of interest will be 
required to have such conflicts managed in a way that is appropriate to their participation in the 
design and conduct of this trial.  The study leadership in conjunction with the National Cancer 
Institute has established policies and procedures for all study group members to disclose all 
conflicts of interest and will establish a mechanism for the management of all reported dualities 
of interest.
9.4
CONFIDENTIALITY AND PRIVACY
Participant confidentiality and privacy is strictly held in trust by the participating investigators, 
their staff, and the sponsor(s). This confidentiality is extended to cover testing of biological 
samples and genetic tests in addition to the clinical information relating to participants. 
Therefore, the study protocol, documentation, data, and all other information generated will be 
held in strict confidence. No information concerning the study or the data will be released to any 
unauthorized third party without prior written approval of the sponsor. 
All research activities will be conducted in as private a setting as possible.
The study monitor, other authorized representatives of the sponsor, representatives of the 
Institutional Review Board (IRB), and/or regulatory agencies may inspect all documents and 
records required to be maintained by the investigator, including but not limited to, medical 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
38
records (office, clinic, or hospital) and pharmacy records for the participants in this study. The 
clinical study site will permit access to such records.
The study participant’s contact information will be securely stored at the/each clinical site for 
internal use during the study. At the end of the study, all records will continue to be kept in a 
secure location for as long a period as dictated by the reviewing IRB, Institutional policies, or 
sponsor requirements.
Study participant research data, which is for purposes of statistical analysis and scientific 
reporting, will be stored at the NCI CCR. This will not include the participant’s contact or 
identifying information. Rather, individual participants and their research data will be identified 
by a unique study identification number. The study data entry and study management systems 
used by the clinical site and by NCI CCR research staff will be secured and password protected. 
At the end of the study, all study databases will be archived at the NIH.
To further protect the privacy of study participants, a Certificate of Confidentiality has been 
issued by the National Institutes of Health (NIH).  This certificate protects identifiable research 
information from forced disclosure. It allows the investigator and others who have access to 
research records to refuse to disclose identifying information on research participation in any 
civil, criminal, administrative, legislative, or other proceeding, whether at the federal, state, or 
local level. By protecting researchers and institutions from being compelled to disclose 
information that would identify research participants, Certificates of Confidentiality help achieve 
the research objectives and promote participation in studies by helping assure confidentiality and 
privacy to participants.
10 PHARMACEUTICAL INFORMATION
10.1 LMB-2 IS AN INVESTIGATIONAL RECOMBINANT IMMUNOTOXIN. 
10.1.1 LMB-2 (NSC 676422)
Other Names: Anti-TAC (Fv) PE 38.
Classification: Recombinant immunotoxin.
Description: LMB-2, a 63-kD single-chain recombinant immunotoxin, is comprised of variable 
regions of the light and heavy chains (Fv) of a murine monoclonal antibody (anti-TAC) against 
the 55-kD subunit of the low affinity interleukin-2 receptor (IL-2R) fused to a truncated 
derivative of Pseudomonas exotoxin, (PE 38).
Mode of Action: The human IL-2R (also known as TAC antigen and CD25) plays an important 
role in lymphocyte differentiation and immune response regulation. It is overexpressed on 
various types of malignant cells and lymphocytes mediating autoimmune disease, making IL-2R 
a potential cancer therapy target. Anti-TAC is a murine monoclonal antibody that binds to the 
IL-2R (with high affinity blocking the interaction of IL-2 with IL-2R. Pseudomonas exotoxin 
(PE) 38 is a truncated portion of a protein secreted by P. aeruginosa that lacks the native cell-
binding site and kills mammalian cells by catalyzing irreversible ADP-ribosylation and 
inactivating elongation factor 2, halting protein synthesis. LMB-2 selectively binds to cells 
bearing IL-2R and is internalized to release PE 38, causing cell destruction.
How Supplied: LMB-2 is available as a sterile frozen solution in phosphate buffered saline and 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
39
may be vialed and labeled at different concentrations (i.e. 420 µg/mL, 436 µg/mL, 484 µg/mL, 
534 µg/mL, etc.). The pH is approximately 7.4.
Manufacturer:  Produced by the Monoclonal Antibody and Recombinant Protein facility, NCI, 
Frederick, MD.
Please note: The concentration of LMB-2 may vary from lot to lot. Please check the label 
for the correct concentration prior to the preparation of each dose.  
Preparation: Thawing instructions: Warm vials in the hand for 10 to 20 seconds before placing 
them in a water bath to thaw. Place vials in a cup of room temperature (15-30ºC) sterile water for 
injection, USP such that when the vial is upright, the water level will be at the neck of the vial. 
Visually inspect the vials after thawing. Do not use if solution appears turbid. Do not shake; 
proteins can foam and may denature.
LMB-2 should only be diluted in 0.2% human serum albumin (HSA) in 0.9% sodium chloride. 
Please note: Particulate matter was found in vials from lots 103037 and 103038 during the 60-
month stability testing. The lots met all other release specifications, including composition and 
potency. Tests were conducted with a Millex GV 25 mm (0.2 micron) filter to remove the 
particulates. Post filtration studies demonstrated minimal loss of potency. LMB-2 undiluted 
solution must be filtered with a 0.2 micron low protein binding Millex GV filter prior to 
adding to the 0.2% HSA in 0.9% sodium chloride.
IV infusion: The required amount of LMB-2 (436 µg/mL, the undiluted vial) will be diluted 
with 0.2% HSA in 0.9% sodium chloride to a total volume of 50 mL in an empty Partial Additive 
Container (PAB®). Filter LMB-2 with a 0.2 micron low protein binding Millex GV filter prior to 
adding to the 0.2% HSA in 0.9% sodium chloride. Agitate gently to disperse.
A PAB® container is a standard, commonly-used parenteral product container that is composed 
of an ethylene and propylene co-polymer without plasticizer. It is an empty sterile bag to which 
pharmacy personnel add the various components specified by the protocol to a specific 
prescribed volume. It is preferred over other plastic containers because it is manufactured 
without polyvinylchloride (PVC) and plasticizers such as di-(2-ethylhexyl) phthalate (DEHP) 
with which some chemotherapy agents interact. 
Storage: Intact vials should be stored in the freezer at -70ºC or below. The intravenous 
admixture should be stored in the refrigerator (2-8ºC). Thawed vials should not be refrozen.
Stability: Intact vials of LMB-2 are stable for at least 5 years when stored at -70ºC. Once 
thawed, intact vials are stable for 24 hours when stored in the refrigerator (2-8ºC) and for 4 hours 
when stored at room temperature (15-30ºC). LMB-2 is stable for 25 hours at 2-8ºC once further 
diluted in 0.2% HSA in 0.9% sodium chloride. Vials cannot be refrozen.
Route(s) of Administration: Intravenous (IV).
Method of Administration: Treatment doses should be infused intravenously over 30 minutes.
Patient Care Implications: If necessary, supportive care for vascular leak syndrome/capillary 
leak syndrome should be instituted and may include fluid and electrolyte management, diuresis, 
albumin, glucocorticoids, and cardiovascular support. Other toxicities should be managed 
clinically.
Anti-emetics or hematologic growth factors are not expected to be required, but are permitted if 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
40
indicated. Patients who develop nausea may be treated with a serotonin 5-HT3 receptor 
antagonist for at least 24 hours after their last episode of nausea. Other antiemetics, such as 
prochlorperazine, metoclopramide, or lorazepam may be used in addition if necessary.
Patients who develop myalgias may be given acetaminophen 650 mg to 1000 mg every 6 hours 
until 24 hours after completing the last dose of LMB-2. 
It may then be given as needed. Patients may receive NSAIDs or narcotics if acetaminophen is 
inadequate. Patients who develop temperatures >38.0ºC may receive scheduled acetaminophen 
650 mg to 1000 mg every 6 hours until 24 hours after completing the last dose of LMB-2. It may 
then be given as needed.
Emergency medications should be available in the treatment unit in the event of an anaphylactic 
reaction. Allergic reactions should be treated acutely with antihistamines, and if needed, with 
glucocorticoids, fluids, and/or epinephrine.
Agent Ordering and Agent Accountability 
NCI supplied agents may be requested by the Principal Investigator (or their authorized 
designee) at each participating institution.  Pharmaceutical Management Branch (PMB) policy 
requires that agent be shipped directly to the institution where the patient is to be treated.  PMB 
does not permit the transfer of agents between institutions (unless prior approval from PMB is 
obtained.) The CTEP assigned protocol number must be used for ordering all CTEP supplied 
investigational agents.  The responsible investigator at each participating institution must be 
registered with CTEP, DCTD through an annual submission of FDA form 1572 (Statement of 
Investigator), Curriculum Vitae, Supplemental Investigator Data Form (IDF), and Financial 
Disclosure Form (FDF).  If there are several participating investigators at one institution, CTEP 
supplied investigational agents for the study should be ordered under the name of one lead 
investigator at that institution.
LMB-2 may be requested by completing a Clinical Drug Request (NIH-986) and mailing it to the 
Pharmaceutical Management Branch (PMB), DCTD, NCI, 9000 Rockville Pike, EPN Room 
7149, Bethesda, MD 20892-7422 or faxing it to (301) 480-4612.  For questions call (301) 496-
5725.
Agent Inventory Records -  The investigator, or a responsible party designated by the 
investigator, must maintain a careful record of the inventory and disposition of all agents 
received from DCTD using the NCI Drug Accountability Record (DAR) Form.  (See the NCI 
Investigator’s Handbook for Procedures for Drug Accountability and Storage.)
10.1.2 Toxicity
10.1.2.1 Preclinical studies
In a GLP toxicology study, 4 Cynomolgus monkeys received 20 µg/Kg days 1, 3 and 5 with no 
significant toxicity.  Another four monkeys were then given 300 µg/Kg days 1, 3 and 5 and 
experienced dose-limiting toxicity with anorexia and 2 to 4-fold transaminase elevations.  The 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
41
LD10 and LD50 in mice were 200 and 257 µg/Kg every other day for 3 doses.  The cause of 
death was liver damage.
10.1.2.2 Phase I and II trials
Adverse events were reported in relationship to treatment cycle.  Grade III-IV toxicities included 
reversible transaminase elevation, fever, CK elevation, cardiomyopathy, thrombocytopenia, 
allergic reaction, and diarrhea.
The most common grade I-II toxicities were transaminase elevation, fever, hypoalbuminemia, 
and fatigue.  Other grade I-II toxicities included vascular leak syndrome/capillary leak syndrome, 
weight gain, hypotension, nausea, pericardial effusion, allergy, proteinuria, and  increased 
creatinine.
10.2 PREMEDICATIONS (ABBREVIATED PHARMACEUTICAL SECTION)
These agents will be provided by the Clinical Center Pharmacy and will be given orally.  Please 
refer to the package inserts for complete pharmaceutical information on these products.
10.2.1 Acetaminophen (Tylenol): 

Side effects are extremely unlikely. Regular use of acetaminophen can cause liver 
damage especially at high doses (>4000 mg/day or >12 regular strength tablets per day).  
To minimize this possibility patients should not take over-the-counter products 
containing acetaminophen during the time periods they are taking scheduled 
acetaminophen doses on this study.
10.2.2 Ranitidine (Zantac): 

Side effects include tiredness, dizziness, headache, and diarrhea.
10.2.3 Hydroxyzine (Atarax): 

Side effects include sleepiness, dizziness, restlessness, and irritability.
10.2.4 Dexamethasone (Decadron):

To be given only prior to the 1st dose of retreatment cycles.  Side effects of a single dose 
of dexamethasone might include difficulty sleeping, increased hunger, and increased 
blood glucose. Steroids are associated with an increased risk of infection although this is 
more applicable to chronic use.
11 CORRELATIVE/SPECIAL STUDIES
Note: tubes and media for research tests may be substituted based on availability with the 
permission of the PI or laboratory investigator.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
42
11.1 ASSAY FOR DETECTING NEUTRALIZING ANTIBODIES TO LMB-2.
11.1.1 Set up SP2/Tac cell plates using SP2/Tac cell line protocols.  Place cell plates in 37C 
incubator until needed.
11.1.2 Using a costar #3799 U-bottom plate, add 72 ul of .2% PBS/HSA to wells A1-A4 and 60 
ul to wells A9-A-12.  Add patient serum, obtained from serum separator or other clot 
tube, to the plate in the following manner:
a.
Patient 1: 45 ul of serum in wells D1-D4
b.
Patient 2: 45 ul of serum in wells E1-E4
c.
Patient 3: 45 ul of serum in wells F1-F4
d.
Patient 4: 45 ul of serum in wells G1-G4
e.
Patient 5: 45 ul of serum in wells H1-H4
11.1.3 Thaw LMB-2 toxin in hand and add 15 ul to well A12. Mix well. Transfer 15 ul from 
well  A12 to well A11. Mix well. Transfer 15 ul from well A11 to well A10. Mix well.  
Well A9 does not receive toxin.
11.1.4 Using a multichannel pipette, transfer 8 ul from wells A9-A12 into wells A1-A4.  Well 
A1 receives from well A9, well A2 from well A10, etc.
11.1.5 In a similar manner, transfer 5 ul from wells A9-A12 into the patient serum wells. For 
example, well D1 receives from well A9, etc. Dispel pipette tips between additions to 
patients samples. 
11.1.6 Using a multichannel pipette, mix wells A1-A4, and all patient sample wells in which   
toxin additions were made. Dispel pipette tips between each set.
11.1.7 Incubate the U-bottom plate at 37C for 15 minutes.
11.1.8 During incubation, prepare two new Costar #3799 U-bottom plates with 200 uL of room 
temperature SP2/Tac cell media in all used rows.
11.1.9 Add 10 uL of .2% PBS/HSA to row C of U-bottom plate #2.
11.1.10
When the 15 minute incubation of plate one is complete, add from plate one to 
plate two in the following manner:
         Using a single channel pipette:
         Transfer 10 ul from plate 1, well A1 to plate 2, wells A1-A6.
         Transfer 10 ul from plate 1, well A2 to plate 2, wells A7-A12.
         Transfer 10 ul from plate 1, well A3 to plate 2, wells B1-B6.
         Transfer 10 ul from plate 1, well A4 to plate 2, wells B7-B12.
         Using a multichannel pipette:


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
43
         Transfer, in triplicate, 10 ul from patients wells of plate 1 into the corresponding wells of 
plate two.  For example, patient #1 sample from well D1 of plate one is transferred to wells 
D1-D3 of plate two.  Patient #1 sample from well D2 is added to wells D4-D6 of plate two, 
etc.  Continue to add samples in this manner until all Patient samples have been added to 
plate 2.
11.1.11
After transfers are complete, mix all wells of plate two using a multichannel 
pipette.  Be sure to dispense tips between rows.
11.1.12
Using a 12-tip multichannel pipette, make direct transfers of 18.5 ul from plate 2 
into plate 3 by row. For example, transfer wells A1-A12 of plate 2 into wells A1-A12 of 
plate 3. Mix plate 3 using a multichannel pipette as explained previously.
11.1.13
As in step 12, make a direct transfer of 50 ul from plate 3 into the cell plates 
prepared before start of assay. Gently add dilution to the cell plate (do not pipette up and 
down).
11.1.14
Incubate the SP2/Tac assay plates 18-20 hours at 37C. Pulse the cell plates using 
leucine-free RPMI media and 3H leucine at a dilution of 1:50 (1 uCi/well). Gently add 50 
ul of the 1:50 {125I}-leucine dilution to the assay plates using a multichannel pipette. 
Dispense tips between rows. Incubate the plates for 41/2 hours at 37C.
11.1.15
The cell plates are harvested after incubation using a MACH III Tomtek cell 
harvester. Harvester instructions located in protocol book in room 428A. The cells are 
harvested onto filter mats and read using a Wallac Beta Counter and the data is 
electronically captured.
Reasons to repeat an assay:
1.
Failure to get counts above 2500.
2.
Failure of the standard curve.
3.
Low counts in serum only tube (as compared to subsequent serum dilutions).
4.
PI requests a repeat of assay.
11.2 BIOASSAY FOR BLOOD LEVELS OF LMB-2  
11.2.1 Plate SP2/Tac cells at 40,000/well in flat bottom plates. A typical PK assay requires 3 
plates (I, II, and III).
11.2.2 Label 2 U-bottom plates (I & II) and add room temperature SP2/Tac media in the 
following fashion:
a.
In U-bottom plate I: wells A1-A5 receive 200 ul, wells A6-A7  receive 198 
ul/well, A8 receives 205 ul, columns 1-3 starting with row B receive 198 ul, and column 
4 receives 205 ul starting with row B.
b.
In U-bottom plate II: wells A1-4 and A7-10 receive 200 ul of media, columns 1-3 
and 7-9 starting with row B receive 198 ul, and columns 4 and 10 starting with row B 
receive 205 ul.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
44
11.2.3 Add 8.5 ul of each plasma time point to column 4 starting with row B of U-bottom plate I 
in sequential order. It is very important to double-check the order of the time points. Day 
1 time points are added first in order of time drawn, then day 3, etc. A total of seven time 
points can be added to U-bottom plate I.
11.2.4 Proceed to U-bottom plate II. Additional time points, in sequential order, can be added 
first to column 4 starting with row B, then to column 10 also starting with row B. Row A 
is left blank in both U-bottom plates as Row A is used for the titration row. Dr. Kreitman 
HSA requested a total of two “blank” rows per PK assay. These rows receive only media 
in the final step of the assay (no toxin or plasma is added to these rows). The assay must 
be structured so that a total of two rows in the flat bottom plates are not used. A total of 
19 time points can be run on three plates. If more than 19 time points are to be run, it will 
be necessary to use an additional cell plate IV.
11.2.5 After the plasma samples are added to the U-bottom plates, an 8 well multichannel 
pipette is used to the dilutions. The same procedure is used on each U-bottom plate 
starting with row B. Transfer 22 ul from column 4 into column 3 (i.e. 22 ul from B4 is 
transferred into B3, etc.). Column 3 is mixed well and 22 ul is transferred from column 3 
into column 2. Finally, 22 ul is transferred from column 2 into column 1. The same 
procedure is used to make dilutions from column 10 through column 7 of U-bottom plate 
II. It is important that thorough mixing is performed after each dilution and before the 
next transfer is made.
11.2.6 Add 8.5 ul of LMB-2 toxin to well A8 of U-bottom plate I. Mix well. Transfer 22 ul of 
A8 into A7, 22 ul of A7 into A6, 50 ul of A6 into A5, 50 ul of A5 into A4, 50 ul of A4 
into A3, 50 ul of A3 into A2, and 50 ul of A2 into A1. Again, thorough mixing is 
necessary in each well before each transfer is made.
11.2.7 Transfer 50 ul from well A5 of U-bottom plate I into wells A4 and A10 of U-bottom 
plate II. A series of 50 ul transfers are made from A4 to A3, from A3 to A2, and from A2 
to A1 with thorough mixing after transfer. Next, 50 ul transfers are made from A10 to 
A9, from A9 to A8, and from A8 to A7. This is to assure a titration curve for each of the 
three cell plates of the PK assay. Once the toxin is added to the U-bottom plates and 
mixed, the final transfer to the cell plate is made. At this point, there should be three sets 
of four columns containing serum and toxin dilutions on the U-bottom plates I and II.
11.2.8 The PK assay does not require a 15 minute incubation before addition of samples to the 
cell plates. Therefore, a multichannel pipette is used to transfer a 50 ul aliquot from each 
column of the U-bottom plates to the SP2/Tac cell plates in triplicate. For example, 50 ul 
from column 1 of U-bottom plate I is added to columns 1-3 of SP2/Tac cell plate I, 
column 2 of U-bottom plate I is added to 4-6 of cell plate I, column 3 to columns 7-9, and 
column 4 to columns 10-12 etc.
11.2.9 Similarly, 50 ul from columns 1-4 of U-bottom plate II are transferred to SP2/Tac cell 
plate II in triplicate and columns 7-10 of U-bottom plate II are added to SP2/Tac cell 
plate III in triplicate. Remember to add 50 ul of media (without toxin or plasma) to the 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
45
rows used as blank rows. If media is not added to these rows, the counts will be slightly 
higher and will not accurately represent the control value.
11.2.10
Incubate the plates for 18-20 hours at 37C.  End of DAY 1.
11.2.11
DAY 2
Following overnight incubation at 37C, the plates are pulsed, harvested, and counted as 
in the neutralization assay.
Reasons to repeat assay:
1.
Failure to get counts above 2500.
2.
Failure of standard curve.
3.
Draw points run in improper order.
4.
Repeat requested by Dr. Kreitman.
11.2.12
Follow Up
For follow-up studies in patients off-treatment, which will not affect eligibility for 
enrollment or retreatment, a non-radioactive neutralization assay may be used, under non-
CLIA conditions.  For additional correlation between the 2 assays, serum samples are 
saved to enable the new assay to be run with historical samples previously tested using 
the radioactive CLIA assay..


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
46
 
12 STUDY CALENDAR
Eligibility 
Evaluation
Every Treatment Cycle
Post-Treatment
Procedures
Prior to 
Cycle 1
Pre-
cycle
Day1
Day 
2
Day 
3
Day 
4
Day 5
Day 
6
Days 
7-8
Week 
2
Week 3
Week 
4
8 
weeks 
after 
CR6
Every 
3-12 
months5
Blood sample for CD25 analysis (by 
FACS)
X
X
X
History (include PS) & physical exam
X
X
Echocardiogram, stress test 
X
ECG, CXR
X
X
CT scan of chest/abdomen/pelvis or 
abdominal MRI1
X
X
X
Serum anti-LMB-2 antibody assay, 
HIV, Hep BsAg & C
X
Pregnancy test if applicable
X
Bone Marrow Biopsy
X
X
Drug administration (LMB-2)
X
X
X
CBC/diff 
X
X
X
X
X
X
X
Acute care panel, hepatic panel, 
mineral panel
X3
X
X
X
X3
X3
Total protein
X
LDH, PT, Beta-2 microglobulin 
(B2M), SPEP, urinalysis
X
LDH, urinalysis, uric acid, CK
X
X
X
Fibrinogen, D-dimer, uric acid, 
amylase, lipase, IgG, IgA, IgM, CRP, 
ferritin, haptoglobin, B2M and SPEP
X
PT, PTT
X
24 hour Urine for CrCl 
X
X
Pharmacokinetic Studies2 
X
X
X
Neutralizing antibodies and soluble 
Tac
X
X4
X7


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
47
1 A CT scan (in patients with an abnormal initial scan) will be done routinely after even cycle numbers (2, 4, 6) and will be done at  
other times if there is clinical evidence that the patient has had a PR, CR, or progressive disease.  Ultrasound can replace CT/MRI in 
the absence of lymph nodes, at the discretion of the PI.
2 Refer to Section 4.3 for PK collection instructions and schedule.
3 Albumin is collected instead of mineral panel.
4 Between Days 17-25.
5 Refer to Section 4.10.
6 Refer to Section 4.4.3.
7 Only for patients with PD 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
48
13 MEASUREMENT OF EFFECT
13.1 RESPONSE CRITERIA
13.1.1 Complete remission (CR) requires all of the following: 
o
No evidence of leukemic cells by routine H/E stains of the peripheral blood and bone 
marrow.  Minimal Residual Disease (MRD): CR with HCL evident in blood by flow 
cytometry (FACS) or in bone marrow by immunohistochemistry.  One negative bone 
marrow biopsy is sufficient to confirm CR providing it is done after the normal blood 
counts qualify for at least 4 weeks.
o
No hepatomegaly, splenomegaly, or lymphadenopathy (not >2 cm in short axis) by 
physical examination and appropriate radiographic techniques.  Adenopathy which 
persisted but became negative by biopsy would be consistent with CR.
o
Normal CBC as exhibited by: Neutrophils  1,500/uL, Platelets  100,000/ul, and 
Hemoglobin  11.0 g/dL without transfusions or growth factors for at least 4 weeks.  
After this 4 week period the bone marrow biopsy and CT must be done to confirm 
CR.
13.1.2 Partial Response (PR) requires all of the following for a period of at least 4 weeks:

The patient must have the following (if abnormal prior to treatment): 
o
 50% decrease in hairy cell count from the pretreatment baseline value by flow 
cytometry. Flow cytometry will not prevent consideration of PR or determine 
relapse from PR if the HCL count by flow cytometry remains below 50 cells/mm3 
and the patient qualifies for PR by other parameters.
o
 50% reduction in abnormal hepatosplenomegaly by CT or physical exam.
o
No growth in lymphadenopathy.  As HCL is not PET-avid, adenopathy which is 
stable is considered not measureable to determine PR, and would not contradict a 
PR proven by other criteria.

and the following: 
o
Neutrophils  1,500/uL or 50% improvement over baseline without growth 
factors for at least 4 weeks,
o
Platelets  100,000/uL or 50% improvement over baseline, &
o
Hemoglobin  11.0 g/dL or 50% improvement over baseline without transfusions 
or growth factors for at least 4 weeks.  The baseline Hgb for patients who are 
transfusion dependent for red cells is assumed to be 6 g/dl.
13.1.3 Progressive disease (PD):  Defined by at least one of the following compared to 
pretreatment: 
o
 25% increase in the sum of the products of the greatest perpendicular 
dimensions of at least two lymph nodes on two consecutive examinations at least 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
49
2 weeks apart (at least one node must be  2 cm) or appearance of new palpable 
lymph nodes, 
o
 25% increase in the size of the liver and/or spleen as determined by 
measurement below the respective costal margin, or appearance of new palpable 
hepatomegaly or splenomegaly that was not previously present, 
o
 50% increase in the number of circulating HCL cells by flow cytometry, and 
>50 cells/mm3. 
13.1.4 Stable disease:  (SD) will be characterized by not meeting any of the criteria outlined 
above. 
13.2 MEASUREMENT OF RESPONSE 
Assessment of clinical response will be made according to the guidelines as outlined above. The 
objective measures of response, such as bone marrow slides and CT scans, will be saved so that 
responses may be audited.  Staging will include a CBC/differential, an interim history and 
physical examination with documentation of measurable disease, and performance status.  
Physical examination should record the diameter, in two planes, of the largest palpable nodes in 
each of the following sites: cervical, axillary, supraclavicular, inguinal, and femoral.  Physical 
examination if relevant can record liver and spleen size as determined by measurement below the 
respective costal margin.  A CT/MRI or U/S (in patients with an abnormal initial scan) will be 
done routinely after even cycle numbers (2, 4, 6, 8, 10) and will be done at other times if there is 
clinical evidence that the patient has had a PR, CR, or progressive disease.
13.3 CONFIRMATION OF RESPONSE & DURATION OF RESPONSE 
13.3.1 Confirmation
The beginning of PR or CR will be considered the earliest time point where all relevant tests are 
consistent with CR or PR, respectively.  CR or PR must be confirmed for at least 4 weeks 
according to the criteria above.  For consideration of CR or PR duration based on the CBC, 
single unacceptable values preceded and followed by acceptable values, or values up to day 12 of 
each cycle may be ignored.
13.3.2 Duration of Response
In patients in CR, the bone marrow biopsy and CT scan should be done at least 4 weeks after the last 
dose of the last cycle, at 6 month intervals for 2 years after CR, and then at 1 year intervals, and if 
positive would indicate relapse rather than invalidation of the CR.  The duration of PR or CR is 
measured from the date a confirmed CR or PR begins, until the date that staging studies are no 
longer consistent with CR or PR.  The duration of overall response will be measured from the date 
that a confirmed response begins, until the date that recurrent or progressive disease is objectively 
documented.  
14 DATA REPORTING / REGULATORY CONSIDERATIONS
Adverse event lists, guidelines, and instructions for AE reporting can be found in Section 7 
(Adverse Events: List and Reporting Requirements).


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
50
14.1 PROCEDURES
This study will be monitored by Clinical Data Update System (CDUS) version 1.X.  Cumulative 
CDUS data will submitted quarterly to CTEP by electronic means. Reports are due January 31, 
April 30, July 31, and October 31.
For data safety and monitoring, all adverse events will be reviewed by the Principal Investigator 
and the research team.  The data manager and research nurses will be responsible for data entry 
and reporting.  Unexpected adverse events and/or serious adverse events will be reported to the 
NCI IRB and the study sponsor as described in section 7. If trends are noted and/or risks warrant 
it, accrual will be interrupted and/or the protocol and/or consent document will be amended 
accordingly.
14.2 STUDY SPONSOR: CTEP
15 STATISTICAL CONSIDERATIONS
15.1 STUDY DESIGN/ENDPOINTS  
This is a nonrandomized phase II study.  The primary objective is to 1) determine the response 
rate of LMB-2 in patients with CD25-positive Hairy Cell Leukemia (HCL).  
15.2 SAMPLE SIZE/ACCRUAL RATE  
The primary objective of this trial will be to determine if LMB-2 can produce responses in a 
reasonable proportion of patients at the current dose and schedule and thus warrant further 
testing. Considering that patients have not been able to to respond well to either cladribine or 
BL22, an overall response rate of 50% would be reasonable, and < 20% would be considered too 
low.  The trial will be conducted using a Simon two-stage optimal design [75].  With alpha=0.10 
(the probability of incorrectly accepting a poor agent), and beta=0.10 (the probability of 
incorrectly rejecting a good agent), we will try to rule out an undesirably low response 
probability of 20% (p0=0.20) in favor of a level indicative of acceptable activity, 50% (p1=0.50). 
Ten evaluable patients will initially be enrolled.  If 0-2/10 demonstrate a CR/PR, then accrual 
will stop and the agent will be considered inactive. If 3+/10 patients have a CR/PR, accrual will 
continue until a total of 17 patients have been enrolled. If 3-5 of 17 have a CR/PR, this will be 
considered insufficient activity, and the agent will not be considered worthy of further 
development. If 6+/17 have a CR/PR, then the agent will be considered active (and at least 
potentially able to produce a CR/PR level consistent with 50%). In addition, if there are 6+/17 
responding patients, up to 8 additional patients overall (maximum 25 evaluable patients overall) 
will be allowed to be entered into this trial to be enrolled to provide additional data for secondary 
endpoints.  Confidence intervals will be constructed about the observed response proportions 
based on a two-stage approach [76]. Under the null hypothesis (p0=0.20), the probability of 
early termination of this trial is 0.68.
Duration of response is also important to evaluate in this population.  A statistical summary of 
the duration of response will be reported, along with the fraction of responding patients who 
achieve a six month response duration, and the corresponding 95% confidence interval. In 
addition, a Kaplan-Meier curve of response duration will be created to illustrate the pattern 
associated with this outcome.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
51
It is expected that 5-10 patients per year can be recruited for enrollment onto this trial.  With a 
goal of 25 evaluable patients, it is expected that 4 years is a reasonable time frame in which to 
accrue all needed subjects. The accrual ceiling will be 55 to account for inevaluable participants 
and screen failures..
Due to LMB-2 supply, no more than a total of 13 patients may be accrued to the protocol until a 
new clinical lot is ready for use in patients.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
52
16 REFERENCES
1.
Cheson BD, Martin A: Clinical trials in hairy cell leukemia. Current status and future directions 
[published erratum appears in Ann Intern Med 1987 Oct;107(4):604]. Ann Intern Med 1987, 
106(6):871-878.
2.
Bouroncle BA, Wiseman BK, Doan CA: Leukemic reticuloendotheliosis. Blood 1958, 13:609-630.
3.
Katayama I, Li CY, Yam LT: Histochemical study of acid phosphatase isoenzyme in leukemic 
reticuloendotheliosis. Cancer 1972, 29(1):157-164.
4.
Golomb HM, Catovsky D, Golde DW: Hairy cell leukemia: a clinical review based on 71 cases. Ann 
Intern Med 1978, 89(5 Pt 1):677-683.
5.
Hassan IB, Hagberg H, Sundstrom C: Immunophenotype of hairy-cell leukemia. Eur J Haematol 1990, 
45(3):172-176.
6.
Robbins BA, Ellison DJ, Spinosa JC, Carey CA, Lukes RJ, Poppema S, Saven A, Piro LD: Diagnostic 
application of two-color flow cytometry in 161 cases of hairy cell leukemia. Blood 1993, 82(4):1277-
1287.
7.
DelGiudice I, Matutes E, Morilla R, Morilla A, OwusuAnkomah K, Rafiq F, AHern R, Delgado J, 
Bazerbashi MB, Catovsky D: The diagnostic value of CD123 in B-cell disorders with hairy or villous 
lymphocytes. Haematologica 2004, 89(3):303-308.
8.
Azemar M, Masche A, Schaefer HE, Unger C: Splenic lymphoma with villous lymphocytes - Difficulties 
in differential diagnosis. Onkologie 1998, 21(3):235-239.
9.
Treton D, Valensi F, Troussard X, Gras G, Flandrin G, Galanaud P, Richard Y: Cytokine response of B 
lymphocytes from splenic lymphoma with villous lymphocytes: Correlation with TNF-RII (p75) and 
CD11c expression. Hematology and Cell Therapy 1996, 38(4):345-352.
10.
Blasinska-Morawiec M, Robak T, Krykowski E, Hellmann A, Urbanska-Rys H: Hairy cell leukemia-
variant treated with 2-chlorodeoxyadenosine--a report of three cases. Leuk Lymphoma 1997, 25(3-
4):381-385.
11.
Dunn P, Shih LY, Ho YS, Tien HF: Hairy cell leukemia variant. Acta Haematologica 1995, 94(2):105-
108.
12.
Wu ML, Kwaan HC, Goolsby CL: Atypical hairy cell leukemia. Arch Pathol Lab Med 2000, 
124(11):1710-1713.
13.
Carson DA, Wasson DB, Beutler E: Antileukemic and immunosuppressive activity of 2-chloro-2'- 
deoxyadenosine. Proc Natl Acad Sci U S A 1984, 81(7):2232-2236.
14.
Beutler E, Carson DA: 2-Chlorodeoxyadenosine: hairy cell leukemia takes a surprising turn. Blood 
Cells 1993, 19(3):559-568; discussion 569-572.
15.
Saven A, Burian C, Koziol JA, Piro LD: Long-term follow-up of patients with hairy cell leukemia after 
cladribine treatment. Blood 1998, 92(6):1918-1926.
16.
Goodman GR, Burian C, Koziol JA, Saven A: Extended follow-up of patients with hairy cell leukemia 
after treatment with cladribine. J Clin Oncol 2003, 21(5):891-896.
17.
Cheson BD, Sorensen JM, Vena DA, Montello MJ, Barrett JA, Damasio E, Tallman M, Annino L, Connors 
J, Coiffier B et al: Treatment of hairy cell leukemia with 2-chlorodeoxyadenosine via the Group C 
protocol mechanism of the National Cancer Institute: a report of 979 patients. J Clin Oncol 1998, 
16(9):3007-3015.
18.
Spiers AS: Pentostatin (2prime prime or minute-Deoxycoformycin): Clinical Pharmacology, Role In 
Cancer Chemotherapy, and Future Prospects. Am J Ther 1995, 2(3):196-216.
19.
Grever M, Kopecky K, Foucar MK, Head D, Bennett JM, Hutchison RE, Corbett WE, Cassileth PA, 
Habermann T, Golomb H et al: Randomized comparison of pentostatin versus interferon alfa-2a in 
previously untreated patients with hairy cell leukemia: an intergroup study [see comments]. J Clin 
Oncol 1995, 13(4):974-982.
20.
Flinn IW, Kopecky KJ, Foucar MK, Head D, Bennett JM, Huchison R, Corbett W, Cassileth P, Habermann 
T, Golomb H et al: Long-term follow-up of remission duration, mortality, and second malignancies in 
hairy cell leukemia patients treated with pentostatin. Blood 2000, 96(9):2981-2986.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
53
21.
Maloisel F, Benboubker L, Gardembas M, Coiffier B, Divine M, Sebban C, Blanc M, Abgrall JF, Lederlin 
P, Harousseau JL et al: Long-term outcome with pentostatin treatment in hairy cell leukemia patients. 
A French retrospective study of 238 patients. Leukemia 2003, 17(1):45-51.
22.
Carbone A, Reato G, Di Celle PF, Lauria F, Foa R: Disease eradication in hairy cell leukemia patients 
treated with 2- chlorodeoxyadenosine [letter]. Leukemia 1994, 8(11):2019-2020.
23.
Filleul B, Delannoy A, Ferrant A, Zenebergh A, Van Daele S, Bosly A, Doyen C, Mineur P, Glorieux P, 
Driesschaert P et al: A single course of 2-chloro-deoxyadenosine does not eradicate leukemic cells in 
hairy cell leukemia patients in complete remission. Leukemia 1994, 8(7):1153-1156.
24.
Jehn U, Bartl R, Dietzfelbinger H, Haferlach T, Heinemann V: An update: 12-year follow-up of patients 
with hairy cell leukemia following treatment with 2-chlorodeoxyadenosine. Leukemia 2004, 
18(9):1476-1481.
25.
Golomb HM, Vardiman J: Hairy cell leukemia: diagnosis and management. CA Cancer J Clin 1978, 
28(5):265-277.
26.
Jansen J, Hermans J, Remme J, den Ottolander GJ, Cardozo PL: Hairy cell leukaemia. Clinical features 
and effect of splenectomy. Scand J Haematol 1978, 21(1):60-71.
27.
Jansen J, Hermans J: Splenectomy in hairy cell leukemia: a retrospective multicenter analysis. Cancer 
1981, 47(8):2066-2076.
28.
Bouroncle BA: The history of hairy cell leukemia: characteristics of long-term survivors. Semin Oncol 
1984, 11(4 Suppl 2):479-485.
29.
Baccarani U, Terrosu G, Donini A, Zaja F, Bresadola F, Baccarani M: Splenectomy in hematology. 
Current practice and new perspectives. Haematologica 1999, 84(5):431-436.
30.
Walsh RM, Heniford BT: Laparoscopic splenectomy for non-Hodgkin lymphoma. J Surg Oncol 1999, 
70(2):116-121.
31.
Mercieca J, Puga M, Matutes E, Moskovic E, Salim S, Catovsky D: Incidence and significance of 
abdominal lymphadenopathy in hairy cell leukaemia. Leuk Lymphoma 1994, 14 Suppl 1:79-83.
32.
Lauria F, Forconi F: Towards the pharmacotherapy of hairy cell leukaemia. Expert Opin Pharmacother 
2004, 5(7):1523-1533.
33.
Kantarjian HM, Schachner J, Keating MJ: Fludarabine therapy in hairy cell leukemia. Cancer 1991, 
67(5):1291-1293.
34.
Kraut EH, Chun HG: Fludarabine phosphate in refractory hairy cell leukemia. Am J Hematol 1991, 
37(1):59-60.
35.
Hagberg H, Lundholm L: Rituximab, a chimaeric anti-CD20 monoclonal antibody, in the treatment of 
hairy cell leukaemia. Br J Haematol 2001, 115(3):609-611.
36.
Lauria F, Lenoci M, Annino L, Raspadori D, Marotta G, Bocchia M, Forconi F, Gentili S, La Manda M, 
Marconcini S et al: Efficacy of anti-CD20 monoclonal antibodies (Mabthera) in patients with 
progressed hairy cell leukemia. Haematologica 2001, 86(10):1046-1050.
37.
Nieva J, Bethel K, Saven A: Phase 2 study of rituximab in the treatment of cladribine-failed patients 
with hairy cell leukemia. Blood 2003, 102(3):810-813.
38.
Thomas DA, O'Brien S, Bueso-Ramos C, Faderl S, Keating MJ, Giles FJ, Cortes J, Kantarjian HM: 
Rituximab in relapsed or refractory hairy cell leukemia. Blood 2003, 102(12):3906-3911.
39.
Robb RJ, Munck A, Smith KA: T cell growth factor receptors.  Quantitation, specificity, and biological 
relevance. J Exp Med 1981, 154:1455-1474.
40.
Mehta R, Shah G, Adler W, Kittur D: Soluble interleukin 2 receptor (sIL-2R) levels in renal transplant 
recipients. Clin Transplant 2004, 18 Suppl 12:67-71.
41.
Waldmann TA, Greene WC, Sarin PS, Saxinger C, Blayney DW, Blattner WA, Goldman CK, Bongiovanni 
K, Sharrow S, Depper JM et al: Functional and phenotypic comparison of human T cell 
leukemia/lymphoma virus positive adult T cell leukemia with human T cell leukemia/lymphoma 
virus negative Sézary leukemia and their distinction using anti-Tac.  Monoclonal antibody 
identifying the human receptor for T cell growth factor. J Clin Invest 1984, 73:1711-1718.
42.
Waldmann TA: The interleukin-2 receptor on malignant cells: a target for diagnosis and therapy. Cell 
Immunol 1986, 99:53-60.
43.
Uchiyama TA, Broder S, Waldmann TA: A monoclonal antibody (anti-Tac) reactive with activated and 
functionally mature human T cells.  I.  Production of anti-Tac monoclonal antibody and distribution 
of Tac (+) cells. J Immunol 1981, 126:1393-1397.
44.
Uchiyama T, Nelson DL, Fleisher TA, Waldmann TA: A monoclonal antibody (anti-Tac) reactive with 
activated and functionally mature human T cells.  II.  Expression of Tac antigen on activated 


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
54
cytotoxic killer T cells, suppressor cells, and on one of two types of helper T cells. J Immunol 1981, 
126:1398-1403.
45.
Leonard WJ, Depper JM, Uchiyama T, Smith KA, Waldmann TA, Greene WC: A monoclonal antibody 
that appears to recognize the receptor for human T-cell growth factor; partial characterization of the 
receptor. Nature 1982, 300:267-269.
46.
Waldmann TA, Goldman CK, Bongiovanni KF, Sharrow SO, Davey MP, Cease KB, Greenberg SJ, Longo 
DL: Therapy of patients with human T-cell lymphotrophic virus I-induced adult T-cell leukemia with 
anti-Tac, a monoclonal antibody to the receptor for interleukin-2. Blood 1988, 72:1805-1816.
47.
Waldmann TA, White JD, Goldman CK, Top L, Grant A, Bamford R, Roessler E, Horak ID, Zaknoen S, 
Kasten-Sportes C et al: The interleukin-2 receptor:  a target for monoclonal antibody treatment of 
human T-cell lymphotrophic virus I-induced adult T-cell leukemia. Blood 1993, 82:1701-1712.
48.
Hwang J, FitzGerald DJ, Adhya S, Pastan I: Functional domains of Pseudomonas exotoxin identified by 
deletion analysis of the gene expressed in E. coli. Cell 1987, 48:129-136.
49.
Allured VS, Collier RJ, Carroll SF, McKay DB: Structure of exotoxin A of Pseudomonas aeruginosa at 
3.0 Angstrom resolution. Proc Natl Acad Sci USA 1986, 83:1320-1324.
50.
Hessler JL, Kreitman RJ: An early step in Pseudomonas exotoxin action is removal of the terminal 
lysine residue, which allows binding to the KDEL receptor. Biochemistry 1997, 36:14577-14582.
51.
Kounnas MZ, Morris RE, Thompson MR, FitzGerald DJ, Strickland DK, Saelinger CB: The 2-
macroglobulin receptor/low density lipoprotein receptor-related protein binds and internalizes 
Pseudomonas exotoxin A. J Biol Chem 1992, 267:12420-12423.
52.
Chiron MF, Fryling CM, FitzGerald DJ: Cleavage of Pseudomonas exotoxin and diphtheria toxin by a 
furin-like enzyme prepared from beef liver. J Biol Chem 1994, 269:18167-18176.
53.
Fryling C, Ogata M, FitzGerald D: Characterization of a cellular protease that cleaves Pseudomonas 
exotoxin. Infect Immun 1992, 60:497-502.
54.
Ogata M, Fryling CM, Pastan I, FitzGerald DJ: Cell-mediated cleavage of Pseudomonas exotoxin 
between Arg279 and Gly280 generates the enzymatically active fragment which translocates to the 
cytosol. J Biol Chem 1992, 267:25396-25401.
55.
Chaudhary VK, Jinno Y, FitzGerald D, Pastan I: Pseudomonas exotoxin contains a specific sequence at 
the carboxyl terminus that is required for cytotoxicity. Proc Natl Acad Sci USA 1990, 87:308-312.
56.
Kreitman RJ, Pastan I: Importance of the glutamate residue of KDEL in increasing the cytotoxicity of 
Pseudomonas exotoxin derivatives and for increased binding to the KDEL receptor. Biochem J 1995, 
307:29-37.
57.
Theuer CP, Buchner J, FitzGerald D, Pastan I: The N-terminal region of the 37-kDa translocated 
fragment of Pseudomonas exotoxin A aborts translocation by promoting its own export after 
microsomal membrane insertion. Proc Natl Acad Sci  USA 1993, 90:7774-7778.
58.
Theuer C, Kasturi S, Pastan I: Domain II of Pseudomonas exotoxin A arrests the transfer of 
translocating nascent chains into mammalian microsomes. Biochemistry 1994, 33:5894-5900.
59.
Carroll SF, Collier RJ: Active site of Pseudomonas aeruginosa exotoxin A.  Glutamic acid 553 is 
photolabeled by NAD and shows functional homology with glutamic acid 148 of diphtheria toxin. J 
Biol Chem 1987, 262:8707-8711.
60.
Kondo T, FitzGerald D, Chaudhary VK, Adhya S, Pastan I: Activity of immunotoxins constructed with 
modified Pseudomonas exotoxin A lacking the cell recognition domain. J Biol Chem 1988, 263:9470-
9475.
61.
Siegall CB, Chaudhary VK, FitzGerald DJ, Pastan I: Functional analysis of domains II, Ib, and III of 
Pseudomonas exotoxin. J Biol Chem 1989, 264:14256-14261.
62.
Kreitman RJ, Batra JK, Seetharam S, Chaudhary VK, FitzGerald DJ, Pastan I: Single-chain immunotoxin 
fusions between anti-Tac and Pseudomonas exotoxin:  relative importance of the two toxin disulfide 
bonds. Bioconjugate Chemistry 1993, 4:112-120.
63.
Chaudhary VK, Queen C, Junghans RP, Waldmann TA, FitzGerald DJ, Pastan I: A recombinant 
immunotoxin consisting of two antibody variable domains fused to Pseudomonas exotoxin. Nature 
1989, 339:394-397.
64.
Batra JK, FitzGerald D, Gately M, Chaudhary VK, Pastan I: Anti-Tac(Fv)-PE40:  a single chain 
antibody Pseudomonas fusion protein directed at interleukin 2 receptor bearing cells. J Biol Chem 
1990, 265:15198-15202.


Abbreviated Title:  LMB-2 for HCL
Version date:  02/07/2023
55
65.
Kreitman RJ, Chaudhary VK, Waldmann T, Willingham MC, FitzGerald DJ, Pastan I: The recombinant 
immunotoxin anti-Tac(Fv)-Pseuodomonas exotoxin 40 is cytotoxic toward peripheral blood 
malignant cells from patients with adult T-cell leukemia. Proc Natl Acad Sci USA 1990, 87:8291-8295.
66.
Kreitman RJ, Chaudhary VK, Waldmann TA, Hanchard B, Cranston B, FitzGerald DJP, Pastan I: 
Cytotoxic activities of recombinant immunotoxins composed of Pseudomonas toxin or diphtheria 
toxin toward lymphocytes from patients with adult T-cell leukemia. Leukemia 1993, 7:553-562.
67.
Saito T, Kreitman RJ, Hanada S-i, Makino T, Utsunomiya A, Sumizawa T, Arima T, Chang CN, Hudson 
D, Pastan I et al: Cytotoxicity of recombinant Fab and Fv immunotoxins on adult T-cell leukemia 
lymph node and blood cells in the presence of soluble interleukin-2 receptor. Cancer Res 1994, 
54:1059-1064.
68.
Kreitman RJ, Bailon P, Chaudhary VK, FitzGerald DJP, Pastan I: Recombinant immunotoxins 
containing anti-Tac(Fv) and derivatives of Pseudomonas exotoxin produce complete regression in 
mice of an interleukin-2 receptor-expressing human carcinoma. Blood 1994, 83:426-434.
69.
Wojcinski ZW, Roslinsky JL, Clarke DW, Schuh JCL, Albassam MA, Lillie LE: Review of spontaneous 
and backround pathologic findings in wild-caught Cynomologus monkeys. Toxicol Pathol 1995, 
23:765.
70.
Kreitman RJ, Wilson WH, Robbins D, Margulies I, Stetler-Stevenson M, Waldmann TA, Pastan I: 
Responses in refractory hairy cell leukemia to a recombinant immunotoxin. Blood 1999, 94:3340-
3348.
71.
Kreitman RJ, Wilson WH, White JD, Stetler-Stevenson M, Jaffe ES, Waldmann TA, Pastan I: Phase I trial 
of recombinant immunotoxin Anti-Tac(Fv)-PE38 (LMB-2) in patients with hematologic 
malignancies. J Clin Oncol 2000, 18:1614-1636.
72.
Kreitman RJ, Squires DR, Stetler-Stevenson M, Noel P, Fitzgerald DJ, Wilson WH, Pastan I: Phase I trial 
of recombinant immunotoxin RFB4(dsFv)-PE38 (BL22) in patients with B-cell malignancies. J Clin 
Oncol 2005, 23(27):6719-6729.
73.
Kreitman RJ, Wilson WH, Bergeron K, Raggio M, Stetler-Stevenson M, FitzGerald DJ, Pastan I: Efficacy 
of the Anti-CD22 Recombinant Immunotoxin BL22 in Chemotherapy-Resistant Hairy-Cell 
Leukemia. New Engl J Med 2001, 345(4):241-247.
74.
Robbins DH, Margulies I, Stetler-Stevenson M, Kreitman RJ: Hairy cell leukemia, a B-cell neoplasm 
which is particularly sensitive to the cytotoxic effect of anti-Tac(Fv)-PE38 (LMB-2). Clin Cancer Res 
2000, 6(2):693-700.
75.
Simon R: Optimal two-stage designs for phase II clinical trials. Controlled clinical trials 1989, 10(1):1-
10.
76.
Atkinson EN, Brown BW: Confidence limits for probability of response in multistage phase II clinical 
trials. Biometrics 1985, 41(3):741-744.

ICF_003.pdf: 
	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<0=<>
??ABCB?DE?FGCHAIJGCKLMNLOMLNO
P	Q	

4R-STTR2UVT42UTSU4W4UWVS-SUV4-TX7Y
	QZ[
\V0]+3/^3+)/_8,`a`7`
bcQ\
U2d85+4*),).8*T3)8*0=U,/)T8.$(e'2Sfg$Wah '
__i,0/0j),=0347 k205)/)e+8)3l4+**W+im+_)8
bc\
4*),).8*4+,/+3
40d03/1
nGJopJBp
40,5+,/6+35)0,1
ONqOrqNONM
st

c
b	
	u
btbcv
wx1V0]+3/^3+)/_8,ya`7`
2d0,+f9&%g9z&g"
S_8)*m3+)/_83{_8)*`,)d`;0e
Td)5.0,5+,/=03_|+5.3)]+583+5+83.d5/i|l8,|)5|+5);,+|/0d+*}l0i|+.)|+)=l0i~0i*|*)m+
/0]+8}83/0=/d+3+5+83.d5/i|l`
Td+3+_8),),;|0.i_+,/~)**,0~|+5.3)]+/d+3+5+83.d5/i|l),_03+|+/8)*`Td)5),=03_8/)0,
5d0i*|]+.0,5)|+3+|]+=03+l0i_8m+l0i3.d0).+`a+_]+350=/d+5/i|l/+8_~)**/8*m~)/dl0i
8]0i//d+),=03_8/)0,),/d)5|0.i_+,/`-0_+}+0}*+d8e+}+350,8*y3+*);)0i5y03+/d).8*]+*)+=5
/d8/_8l*)_)//d+m),|50=_+|).8*033+5+83.d),/+3e+,/)0,5),~d).d/d+l~0i*|~8,//0
}83/).)}8/+`T8m+/d+/)_+l0i,++|/085m8,li+5/)0,58,||)5.i55/d)55/i|l~)/d5/8==y8,|
~)/dl0i3=8_)*ly=3)+,|5y8,|}+350,8*d+8*/d.83+}30e)|+35`
=/d+),|)e)|i8*]+),;85m+|/0}83/).)}8/+),/d)53+5+83.d5/i|l)5,0/8]*+/0;)e+.0,5+,/=03
/d+_5+*e+5yl0iy85/d+W+;8**lUi/d03)+|V+}3+5+,/8/)e+y~)**]+/d+)3|+.)5)0,_8m+38,|l0i
83+]+),;85m+|/0;)e+}+3_)55)0,=03/d)5}+350,/0]+),/d)55/i|l`(03/d+3+_8),|+30=/d)5
|0.i_+,/y/d+/+3_l0i3+=+35/0l0i85/d+|+.)5)0,_8m+38,|:03/d+),|)e)|i8*]+),;85m+|/0
}83/).)}8/+),/d)53+5+83.d`
c
b	t
	
tbc
Y0i_8l.d005+,0/
/0
/8m+
}83/
),/d)55/i|l
=038,l
3+850,`
=l0i
0),
/d)5
5/i|lyl0i
_8l
.d8,;+
l0i3_),|8,|5/0}}83/).)}8/),;),/d+5/i|l8/8,l/)_+8,|=038,l3+850,`,+)/d+3.85+yl0i
~)**,0/*05+8,l]+,+=)/5/0~d).dl0i83+0/d+3~)5++,/)/*+|`0~+e+3y/0]+5++,8//d+yl0i
_i5/]+/8m),;}83/),85/i|l0383+]+),;.0,5)|+3+|=0385/i|l`=l0i|0.d005+/0*+8e+/d+
5/i|ly}*+85+),=03_l0i35/i|l/+8_/0+,5i3+858=+~)/d|38~8*=30_/d+3+5+83.d`
stctbcu[
v
Td)5)58.*),).8*/3)8*=03/d+/3+8/_+,/0=d8)3l.+***+im+_)8$4W'~)/d8,+j}+3)_+,/8*|3i;
.8**+|Wah `Wah )583+.0_]),8,/)__i,0/0j),/d8/d85]++,5d0~,/0m)***+im+_)88,|
*l_}d0_8.8,.+3.+**5/d8/d8e+8}30/+),0,/d+)35i3=8.+.8**+|47 k`T0]++*);)]*+=03
/3+8/_+,/0,/d)55/i|ll0i3*+im+_)8.+**5_i5/d8e+47 k0,/d+)35i3=8.+`0~+e+3y/d+




	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<0=>?
@@BCDC@EF@GHDIBJKHDLMNOMPNMOP
Q	R	

4S-TUUS2VWU42VUTV4X4VXWT-TVW4-UY7Z
[3+5+,.+0=47 \0,]0^3*+^_+`)..+**5a0+5,0/+,5^3++,30**`+,/0,/b+[30/0.0*cd+[*8,/0
),.*^a+8/`05/ "[8/)+,/50,/b)5/3)8*c
Xef )58,
+g[+3)`+,/8*
a3^;
/b8/
)5
.0,5)a+3+a
/0
h+
8
3+.0`h),8,/
)``^,0/0g),c
T8.b
Xef 
`0*+.^*+)5`8a+^[0=/i0[83/518[30/+),[83//b8/h),a503/83;+/58.8,.+3.+**8,a8/0g),$8
/][+0=[0)50,'[83//b8/_)**5/b+.8,.+3.+**/0ib).b)/h),a5cUb+h),a),;[83/)5a+3)j+a=30`8
[30/+),/b8/)5,8/^38**][30a^.+ah]`).+cUb+/0g),[03/)0,0=Xef )5,8/^38**][30a^.+ah]
h8./+3)8cXef )58[30/+),/b8/)5[30a^.+ah].0,,+./),;/b+[83/0=/b+`0^5+;+,+3+5[0,5)h*+
=03[30a^.),;/b+h),a),;[30/+),/0[83/0=/b+/0g),;+,+cd+h+*)+j+/b8//b+h),a),;[83/0=
Xef i)**5+*+./)j+*]/83;+/8,a_)**/b+.8,.+3.+**5/b8/b8j+47 \0,/b+)35^3=8.+c,
*8h038/03]+g[+3)`+,/5kXef b85h++,5b0i,/0_)**47 \.0,/8),),;.+**50^/5)a+8b^`8,
h0a]8,a)/b85.8^5+a85);,)=).8,/a+.3+85+),/b+5)l+0=/^`035),`).+/b8/i+3+;)j+,a05+5
5)`)*83/0/b05+^5+a),/b+=)35/b^`8,/3)8*0=Xef c
V[3+*)`),83]5/^a]0=Xef b85h++,[+3=03`+a8//b+8/)0,8*48,.+3,5/)/^/+$4'),ib).b
m![8/)+,/5i)/bj83)0^5*+^_+`)858,a*]`[b0`85i+3+/3+8/+ac,/b8//3)8*k8[83/)8*3+5[0,5+
i850h5+3j+a
),
&
0=
n
[8/)+,/5
i)/b
4XXc

28/)+,/5
i)/b
0/b+3
.8,.+35
),.*^a),;
b8)3]
.+**
*+^_+`)8
$%[8/)+,/5'k.^/8,+0^5U.+***]`[b0`8$&'k8a^*/U.+***+^_+`)8:*]`[b0`8$&'k8,a0a;_),o5
*]`[b0`8$&'b8a3+a^./)0,),/b+)3/^`0358*50c
pqrsOtCDKHIDuH
Xef i)**0,*]h+;)j+,/0[8/)+,/58//b+4*),).8*4+,/+3c28/)+,/5`^5/b8j+3+.+)j+a
[3)03.*8a3)h),+8/*+85/%i++_5[3)03/0+,30**`+,/k8,ab8j++)/b+33+.+)j+a03a+`0,5/38/+a
),+*);)h)*)/]=03fX  cT8.b.].*+0=Xef )5;)j+,h]8,),/38j+,0^5$),/08j+),'),=^5)0,
+j+3]0/b+3a8]=03ma05+5$a8]5&kmk\'cZ0^i)**3+.+)j+^[/0v.].*+50=Xef +j+3]%i++_5
^,*+55]0^a+j+*0[i035+,),;0=a)5+85+k5+3)0^55)a++==+./5k03j0*^,/83)*]i)/ba38ic
V5`8**8`0^,/0=h*00a$^[/0&9/+85[00,5'i)**h+a38i,h+=03+ka^3),;k8,a8=/+3/3+8/`+,/c
Ub+5+h*00a/+5/58**0i^5/0`+85^3+b0i`^.bXef )5),]0^3h*00ak/b++==+./50=Xef 
0,]0^3.8,.+3.+**5),]0^3h*00ak8,a`0,)/03=035)a++==+./5cd+i)**8*50a0h*00a/+5/5[3)03
/0+8.b.].*+8,aa^3),;+8.b.].*+/0_,0ib0i]0^3)``^,+5]5/+`)5),/+38./),;i)/bXef
 c
f+=03++8.b.].*+8,a),=0**0i^[j)5)/5]0^i)**^,a+3;03+[+8/a)5+85++j8*^8/)0,cUb)5i)**
),.*^a+8.83+=^*[b]5).8*+g8`),8/)0,kh*00a/+5/5k.b+5/w38]k8,a+*+./30.83a)0;38`$/+5/0=
]0^3b+83/'c23)03/0/b+=)35/.].*+]0^i)**b8j+8.0`[^/+a/0`0;38[b]$4U'5.8,8,a8,
+.b0.83a)0;38`$^*/3850^,a0=]0^3b+83/'cZ0^i)**8*50b8j+8h0,+`8330ih)0[5]c=/b+5+
5/^a)+5b+*[^5^,a+35/8,ab0i]0^3*+^_+`)8)53+8./),;/0Xef ki+`8]85_=03]0^3
[+3`)55)0,/03+[+8//b+5+/+5/58;8),[3)03/00/b+3.].*+5c
Ub+),=^5)0,0=Xef /8_+5m9`),^/+5cZ0^i)**8*503+.+)j+8*)/+3$8h0^/n.^[5'0==*^)a
/b30^;b8,603.+,/38*j+,0^5.8/b+/+3h+=03+8,a8=/+3+8.ba05+0=Xef cV.+,/38*j+,0^5
.8/b+/+3$464')58[*85/).6/^h+/b8/)5[*8.+a),8*83;+j+),/b8/*+8a5/0/b+b+83/cZ0^`8]
8*3+8a]b8j+8464),[*8.+c=,0/ka+[+,a),;0,/b+5)l+0=]0^383`j+),5k0,+`8],++a/0h+
[*8.+a[3)03/0/3+8/`+,/cV464`8_+5/3+8/`+,/0,/b)55/^a]+85)+38,a*+55[8),=^*h]
a+.3+85),;/b+,++a=03658,a,++a*+5/)._5/0a38ih*00ac=8464)53+x^)3+a033+x^+5/+ak
]0^i)**h+85_+a/03+j)+i8,0/b+3.0,5+,/=03`8,a;)j+.0,5+,/[3)03/0)/5[*8.+`+,/c
yz{}~{z
yz{z


	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<0=>?
@@BCDC@EF@GHDIBJKHDLMNOMPNMOP
Q	R	

4S-TUUS2VWU42VUTV4X4VXWT-TVW4-UY7Z
Z0[\)**3+.+)]+/^+=)35/._.*+858,),`8/)+,/$8ab)//+a/0/^+^05`)/8*'c-[d5+e[+,/._.*+5b8_
d+;)]+,850[/`8/)+,/$,0/8ab)//+a/0/^+^05`)/8*'c=/^+),=[5)0,583+\+**/0*+38/+af_0[b8_
3+/[3,^0b+8=/+38d0[/&\++g$`055)d*_*0,;+3)=.0b`*).8/)0,50..[3'cV=/+33+/[3,),;^0b+f
_0[\)**^8]+d*00a/+5/5a0,+\++g*_8,a/^+3+5[*/5\)**d+=8h+a/0[5d__0[3*0.8*`^_5).)8,c
7[3),;/^+.0[35+0=/^)55/[a_f_0[b8_8*503+e[)3+0/^+3/3+8/b+,/55[.^85/38,5=[5)0,58,a
8,/)d)0/).5c05`)/8*)i8/)0,b8_d+,++a+a)=.0b`*).8/)0,5a+]+*0`c=/^+3+)5+])a+,.+/^8/
/^+38`_\)/^Xjk )5,0*0,;+3+==+./)]+f)/\)**d+a)5.0,/),[+ack*00a\)**d+.^+.g+a`3)03/0
+8.^._.*+0=Xjk /0a+/+3b),+)=/^+*+]+*0=,+[/38*)i),;8,/)d0a)+5)5/00^);^/0;)]+/^+
._.*+0=Xjk c=)/)5/00^);^f=[3/^+3Xjk \)**d+a)5.0,/),[+ac
lmnn
,03a+3/0a+/+3b),+)=_0[83++*);)d*+=03/^)5+h`+3)b+,/8*/^+38`_f5+]+38*/+5/5\)**^8]+/0d+
a0,+cU^)5`+3)0a0=+]8*[8/)0,b8_/8g+5+]+38*\++g58,a\)**b05/*)g+*_d+a0,+858,
0[/`8/)+,/cU^+5+/+5/5b8_),.*[a+5/8,a83ad*00a8,a[3),+/+5/5f8,+*+./30.83a)0;38b/+5/0=
_0[3^+83/f8.^+5/o38_f8,+.^0.83a)0;38bf\^).^)58,[*/3850[,a0=/^+^+83/f.0b`[/+3)i+a
/0b0;38`^_$4U034VU'5.8,5fo38_5f,[.*+83b+a).),+5/[a)+5f8,a8d0,+b8330\d)0`5_c
Vab),)5/38/)0,0=Xjk \)**d+/^30[;^8.+,/38*]+,0[5.8/^+/+3038`+3)`^+38*c6cU^+464
)5),5+3/+ad_+h`+3)+,.+a5/8==[5),;*0.8*8,+5/^+5)8cU^+3)5g58550.)8/+a\)/^/^+`30.+a[3+
),.*[a+`8),fd*++a),;f),=+./)0,f8,aa+]+*0`b+,/0=8)3),/^+.^+5/c0\+]+3f/^+5+
.0b`*).8/)0,583+383+cV)3),/^+.^+5/0[/5)a+/^+*[,;b8_3+e[)3+/+b`0383_`*8.+b+,/0=8
.^+5//[d+d_85[3;+0,cU^+3)5g50=.^+5//[d+`*8.+b+,/),.*[a+`8),fd*++a),;f8,a),=+./)0,c
S/^+33)5g5
0=
/^+
.8/^+/+3
),.*[a+
),=+./)0,
8,a
.*0//),;
0=
_0[3
]+),5f
\^).^
.0[*a
3+e[)3+
3+b0]8*
0=/^+.8/^+/+3=03/3+8/b+,/cU^+5+3)5g5\)**d++h`*8),+a/0_0[),b03+a+/8)*8//^+/)b+0=
),5+3/)0,cp^+,8`+3)`^+38**),+)5[5+af/^+3+)585b8**3)5g0=),=+./)0,f.*0/03d*++a),;8//^+
5)/+0=/^+6*),+cU^+3+)58*5083)5g0=50b+0=/^+a3[;*+8g),;0[/f03+h/38]858/),;c=/^8/
0..[35/^+3+b8_d+50b+a+5/3[./)0,0=5g),/)55[+),8*)b)/+a83+8c28/)+,/583+[3;+a/08*+3/
/^+5/[a_`^_5).)8,5
8/
/^+
=)35/
5);,
0=
8,_
5g),
.^8,;+5f
=03
+h8b`*+
3+a,+55
03
/+,a+3,+55f
830[,a
/^+),=[5)0,5)/+d[/8*50\)/^8,_a)5.0b=03/),/^+),]0*]+a+h/3+b)/_85\+**c=/^+3+)58,_
+])a+,.+0=/0h).)/_=30b*+8g),;f/^+),=[5)0,\)**d+^+*a[,/)*8.+,/38**),+.8,d+`*8.+a=03
/^+),=[5)0,0=a3[;c,8aa)/)0,f8,_/0h).+==+./5/0/^+5g),\)**d+/3+8/+a/0/^+=[**+5/+h/+,/
`055)d*+c
qrstOu
U^+3+)5*)b)/+a+h`+3)+,.+\)/^Xjk ),^[b8,5c,/^+2^85+/3)8*f8/0/8*0=v!`8/)+,/5
3+.+)]+awx._.*+50=Xjk cS,/^8//3)8*f8**5)a++==+./50=Xjk \+,/8\8_\^+,Xjk 
\855/0``+ac,50b+.85+5/^)53+e[)3+a8aa)/)0,8*b+a).8*/3+8/b+,/5cU^+=0**0\),;*)5/0=5)a+
+==+./5),.*[a+5/^05+5++,0,/^+Xjk /3)8*),8a[*/58,a/^05+5++,\)/^5)b)*83)bb[,0/0h),
a3[;5c
yRQz{|}
~ V,+b)8\^).^b8_3+e[)3+d*00a/38,5=[5)0,
~ +83/=8)*[3+\^).^b8_.8[5+5^03/,+550=d3+8/^f5\+**),;0=8,g*+5f8,a/)3+a,+55
~ (*[)a),/^+d0a_\^).^b8_.8[5+*0\d*00a`3+55[3+f5^03/,+550=d3+8/^f8,a5\+**),;
0=8,g*+5




	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<0=>?
@@BCDC@EF@GHDIBJKHDLMNOMPNMOP
Q	R	

4S-TUUS2VWU42VUTV4X4VXWT-TVW4-UY7Z
[ V\,03]8*^+83/\+8/
[ _*08/),;
[ 7+.3+85+
),
^+83/`
5
8\)*)/a
/0
bc]b
\*00d
dc3),;
/^+
e8./)f+e
b^85+
0=
/^+
^+83/\+8/
$5a5/0*+'
[ 7)833^+8
[ 8c5+803/^+c3;+/0f0])/
[ 60])/),;
[ -g+**),;0=/^+\0da
[ (8/);c+03/)3+d,+55
[ (+f+3
[ V\,03]8*3+8./)0,0=/^+\0da/05c\5/8,.+5h.8**+d8**+3;+,5h/^8/83+.0,/8./+d/^30c;^
/^+5i),h),^8*+d),/0/^+*c,;5h5g8**0g+dh03),j+./+d$8**+3;).3+8./)0,'g^).^]8a.8c5+
385^h*0g\*00db3+55c3+hg^++k),;h5^03/,+550=\3+8/^h8,d5g+**),;0=/^+=8.+03/^308/
[ _3c)5),;03\*++d),;
[ 28),
[ ,.3+85+d\*00d*+f+*0=8*)f+3+,ka]+$VXU:-l2U'
[ ,.3+85+d\*00d*+f+*0=8*)f+303\0,++,ka]+$8*i8*),+b^05b^8/85+'
[ ,.3+85+d\*00d*+f+*0=8*)f+3+,ka]+$V-U:-lSU'
[ ,.3+85+d\*00d*+f+*0=+,ka]+$.3+8/),+b^05b^0i),85+'=30]]c5.*+
[ ,.3+85+d\*00d*+f+*0=.3+8/),),+$85c\5/8,.+,03]8**a+*)]),8/+d\a/^+i)d,+a5),/0
/^+c3),+'
[ 7+.3+85+d,c]\+30=8/ab+0=\*00d.+**/^8/^+*b/0.*0/\*00d$b*8/+*+/'
[ m+);^/;8),h*0550=8bb+/)/+
[ 7)kk),+55h^+8d8.^+
[ 7+.3+85+d*+f+*50=8\*00db30/+),.8**+d8*\c]),
[ nc5.*+b8),
[ _*00d),/^+c3),+
[ n03+b30/+),),/^+c3),+/^8,c5c8*h0=/+,85);,0=i)d,+ad)5+85+
[ -^03/,+550=\3+8/^
[ ,.3+85+),/^+,c]\+38,d5)k+0=/^+b03+5),/^+.8b)**83)+5$5]8**\*00df+55+*5'g^).^
.8c5+5*+8i8;+0==*c)d=30]/^+\*00d/0/^+/)55c+5b8.+5h3+5c*/),;),d8,;+30c5*a*0g
\*00db3+55c3+h5g+**),;8,d]c*/)b*+03;8,=8)*c3+
[ X0g\*00db3+55c3+g^).^]8a.8c5+=++*),;=8),/
opqstuqvpwxyzx{|x
opq}~~p}}vwx{yx{


	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<0=>?
@@BCDC@EF@GHDIBJKHDLMNOMPNMOP
Q	R	

4S-TUUS2VWU42VUTV4X4VXWT-TVW4-UY7Z
V.0[[0,5)\++==+./0=)[[],0/0^),\3];55)[)*83/0X_` )5a85.]*83*+8b5c,\30[+def+3+
=*])\*+8b50]/0=g*00\a+55+*5),/0/f+5b),d*],;5d8,\0/f+303;8,5h-c[i/0[5),.*]\+5e+**),;d
\+.3+85+\g*00\i3+55]3+d03\)==).]*/cg3+8/f),;hUf)5.8,g+5+a+3+d8,\8*/f0];fa85.]*83*+8b
5c,\30[+]5]8**c;+/5g+//+3d)/[8c3+j])3+),/]g8/)0,8,\.8,g+=8/8*hS/f+35)\++==+./5
8550.)8/+\e)/f)[[],0/0^),5),.*]\++\+[8$5e+**),;'d8.f+58,\i8),50=/f+[]5.*+5dk0),/5d
8,\:03g0,+5d
f+8\8.f+d
=8/);]+d
\)ll),+55d
g*]33+\
a)5)0,d
*0e+3),;
0=
,03[8*
g*00\
.+**5
),.*]\),;
/f+3+\.+**5$e)/f3)5b0=8,+[)8'def)/+.+**5$e)/f3)5b0=),=+./)0,'d8,\i*8/+*+/5$e)/f3)5b0=
g*++\),;'d8g,03[8*g*00\.*0//),;/+5/58,\3)5b0=g*++\),;d[]5.*+\8[8;+d\)833f+8d
.0,5/)i8/)0,d5/0[8.f03),/+5/),8*]*.+35d5/0[8.fi8),d),\);+5/)0,d\+fc\38/)0,db)\,+c\8[8;+d
8g,03[8*g*00\58*/*+a+*5d=*])\*+8b),/f+*],;5e)/f5f03/,+550=g3+8/fd),=*8[[8/)0,0=/f+
i8,.3+85;*8,\$/f+03;8,),a0*a+\),\)8g+/+5'd.f)**5d\+.3+85+\=],./)0,0=/f+/fc30)\;*8,\d
8,\,+]30*0;).i30g*+[5),.*]\),;5*++i),+55d\+.3+85+\*+a+*0=8*+3/,+55de+8b,+55di8),=]*
/),;*),;$mi),58,\,++\*+5n'd,][g,+55$\+.3+85+\=++*),;'d8,\.0[8h
V.0,\)/)0,b,0e,85f+[0*c/).]3+[).5c,\30[+$Y-'f85g++,5++,e)/f3+*8/+\)[[],0/0^),
\3];5hY-)58i0/+,/)8**c=8/8*i30g*+[/f8/.8,.8]5+=+a+3d8,+[)8$*0e3+\g*00\.+**.0],/'d
/f30[g0.c/0i+,)8$*0ei*8/+*+/.0],/'dg*++\),;d5/30b+d8,\b)\,+c=8)*]3+hU3+8/[+,/0=5+a+3+
Y-),.*]\+58i30.+\]3+b,0e,85i*85[8+^.f8,;+03i*85[8if+3+5)5def+3+/f+*)j])\i03/)0,
0=/f+g*00\$i*85[8')53+[0a+\=30[/f+g0\c8,\3+i*8.+\e)/fi*85[8=30[g*00\\0,035]5),;
85i+.)8*[8.f),+hTa+,e)/f/3+8/[+,/dY-[8c*+8\/0\+8/f03i+3[8,+,/b)\,+c8,\:03g38),
\8[8;+hV\a+35+3+8./)0,58550.)8/+\e)/fi*85[8if+3+5)583+383+d8,\83+;+,+38**c[)*\hUf+c
),.*]\+i8),8,\g3])5),;8//f+),5+3/)0,5)/+0=/f+),/38a+,0]5*),+d8,\8/+[i0383c\+.3+85+),
/f+i*8/+*+/.0],/8,\:033+\g*00\.+**.0],/h(8),/),;+i)50\+53+*8/+\/0,++\*+),5+3/)0,.8,
0..]3d8,\5b),/),;*),;.8]5+\gc*0e.8*.)][*+a+*5.8,383+*c0..]3h,/+33]i/),;/f+
i*85[8if+3+5)5i30.+\]3+.8,3+a+35+/f)5*8//+33+8./)0,h7]3),;i*85[8if+3+5)5d8/*+85//e0
,]35+5e)**g+i3+5+,/d8,\8g*00\g8,bifc5).)8,e)**g+8a8)*8g*+),/f+.*),).83+8ef+3+/f+
i30.+\]3+)5i+3=03[+\h
X_` 8,\0/f+35)[)*83\3];5.8,.8]5+8**+3;).3+8./)0,5/f8/[8c38,;+=30[[)*\/05+a+3+h
-c[i/0[50=8**+3;).3+8./)0,5[8c),.*]\+f)a+5$3+\385fe)/fg][i5def+8*5d03e+*/5'd8,\
0/f+35b),385f+5d5e+**),;d)/.f),;d=+a+3d.f)**5d*0eg*00\i3+55]3+d=85/f+83/38/+def++l),;d
5f03/,+550=g3+8/fd8,\383+*cd\+8/fh,8,8//+[i//0\+.3+85+/f+3)5b0=5].f3+8./)0,5dc0]e)**
g+;)a+,8,][g+30=8\\)/)0,8*[+\).8/)0,5$m23+[+\).8/)0,5n'g+=03+8,\8=/+3+8.f\05+0=
X_` h
28/)+,/5e)/f4X0=/+,f8a+*0eg*00\.0],/58,\3+j])3+3+\g*00\.+**8,\:03i*8/+*+/
/38,5=]5)0,5de)/f8550.)8/+\3)5b5),.*]\),;/38,5=]5)0,3+8./)0,58,\),=+./)0,5$5].f8568,\
f+i8/)/)5'h23)03/3+8/[+,/[8cf8a+e+8b+,+\c0]3)[[],+5c5/+[h/)5i055)g*+/f8/X_` 
[8c8*50e+8b+,c0]3)[[],+5c5/+[h,=+./)0,5/f8/\+a+*0i),),\)a)\]8*5e)/f.8,.+3.8,g+
a+3c5+3)0]5hZ0]5f0]*\5++b)[[+\)8/+[+\).8*8//+,/)0,=03=+a+30a+3&9&o($pqhpo4'038,c
5);,50=),=+./)0,h
ErstsussvwrKHDxyrHzEv{Hr|D}CvwDx{CDs~
JvvxCKyr|~U0[0,)/03/f++==+./50=/f+38ic=3+j]+,/g*00\/+5/5e)**g+,+.+5583chYi/08
0,+],)/$8g0]/9/+85i00,5'0=g*00\d[8cg+\38e,+a+3ce++b5=033+5+83.fi]3i05+5ef)*+
c0]83+i83/).)i8/),;),/f+5/]\chTa+3c+==03/e)**g+[8\+/0b++ig*00\/+5/5/08[),)[][h
Z0]e)**g+[0,)/03+\=038,+[)88,\;)a+,g*00\/38,5=]5)0,5)=,++\+\h-)\++==+./50=g*00\




	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<0=><
??ABCB?DE?FGCHAIJGCKLMNLOMLNO
P	Q	

4R-STTR2UVT42UTSU4W4UWVS-SUV4-TX7Y
Z38[5),.*\Z+]8),8,Z^3\)5),;),/_+83+8[_+3+/_+,++Z*+[85]*8.+Z`*);_/_+8Z+Z,+55`8,Z
383+*a`=8),/),;b
cdeCfJBBdghCiGij=8^0,+k8330[85])38/)0,)5Z0,+`a0\3_)]^0,+[)**^+,\k^+Z[)/_
8,+5/_+5)8`85k8**,++Z*+[)**^+),5+3/+Z),/0/_+_)]^0,+`8,Z8^0\//[0/8^*+5]00,50=^0,+
k8330[[)**^+3+k0l+Z/_30\;_/_+,++Z*+bT_)5]30.+Z\3+\5\8**a.8\5+50,*a^3)+=Z)5.0k=03/b
6+3a383+*a`),=+./)0,03^*++Z),;k8a0..\38//_+,++Z*+5)/+b
EHJmnemoipJei1Y0\k8a3+.+)l+8.0,/385/8;+,/),q+./+Z),/0a0\383k85]83/0=a0\35.8,b
40,/385/8;+,/5.8,.8\5+8**+3;).3+8./)0,58,Zr)Z,+aZ8k8;+bU**+3;).3+8./)0,5.8,),.*\Z+
k)*Z)/._),;8550.)8/+Z[)/__)l+5^\/.8,8*503+5\*/),85+3)0\5*)=+/_3+8/+,),;+k+3;+,.a
=30kZ)==).\*/a^3+8/_),;b=/_)50..\35`)/)5/3+8/8^*+bY0\k8a=++*Z)5.0k=03/[_+,/_+
.0,/385/k8/+3)8*)5),q+./+ZbY0\k8a=++*[83k`=*\5_+Z`;+/8k+/8**)./85/+),a0\3k0\/_03`
383+*a`k8ak8r+a0\l0k)/03=++*5).r/0a0\35/0k8._b2*+85+85r/_+5/\ZaZ0./03)=a0\
_8l+s\+5/)0,58^0\//_+3)5r50=/_+5+5.8,5b
fDE1t8;,+/).3+50,8,.+)k8;),;$tV'\5+585/30,;k8;,+/).=)+*Z8,Z38Z)0[8l+5/0/8r+
])./\3+50=/_+^0Zabu+[)**0^/8),])./\3+50=a0\3._+5/`8^Z0k+,8,Z]+*l)5=03/_)55/\Zab
T_+tV5.8,,+3)58k+/8*.a*),Z+35\330\,Z+Z^a85/30,;k8;,+/).=)+*Zb2+0]*+83+8/3)5r
=03),q\3a=30k/_+tVk8;,+/)=/_+a_8l+50k+r),Z50=k+/8*),/_+)3^0Zab/k8a^+\,58=+
=03a0\/0_8l+8,tV5.8,)=a0\_8l+]8.+k8r+35030/_+3)k]*8,/+Z+*+./3).8*Z+l).+5`^38),
5/)k\*8/035`50k+/a]+50=Z+,/8*)k]*8,/5`8,+\3a5k.*)]5$k+/8*.*)]50,/_+[8**0=8*83;+
83/+3a'`k+/8*]305/_+5+5$),.*\Z),;k+/8*]),58,Z30Z5`_+83/l8*l+5`8,Z.0._*+83)k]*8,/5'`
]+3k8,+,/+a+*),+3`/8//005`8,)k]*8,/+ZZ+*)l+3a]\k]`035_38],+*=38;k+,/5bu+*Z+358,Z
k+/8*[03r+35k8a_8l+5k8**k+/8*=38;k+,/5),/_++a+bY0\[)**^+5.3++,+Z=03/_+5+
.0,Z)/)0,5^+=03+_8l),;8,atV5.8,b=a0\_8l+8s\+5/)0,8^0\/k+/8*),a0\3^0Za`a0\
5_0\*Z),=03k/_+5/8==bY0\[)**^+85r+Z/0.0k]*+/+8,tV5.3++,),;=03k^+=03++8._tV
5.8,a0\_8l+b
,8ZZ)/)0,`8**k8;,+/).0^q+./5$*)r+[8/._+5`.0),5`q+[+*3a`8,Z.3+Z)/.83Z5'k\5/^+3+k0l+Z
^+=03++,/+3),;/_+tV5.8,300kb
2+0]*+[)/_=+830=.0,=),+Z5]8.+5k8a^+.0k+8,v)0\5Z\3),;8,tVbT_05+[)/_^8.r
]30^*+k5k8a_8l+^8.r]8),03Z)5.0k=03/=30k*a),;),/_+5.8,,+3bT_+,0)5+=30k/_+
5.8,,+3)5*0\Z+,0\;_/0Z8k8;+_+83),;`+5]+.)8**a),]+0]*+[_08*3+8Za_8l+_+83),;*055b
Sl+3a0,+_8l),;83+5+83._tV5.8,[)**^+=)//+Z[)/__+83),;]30/+./)0,b=/_+_+83),;
]30/+./)0,.0k+5*005+Z\3),;/_+5.8,`a0\5_0\*Z*+/\5r,0[3);_/8[8ab
T_+3+83+,0r,0[,*0,;/+3k3)5r50=tV5.8,5b
DniwixBdHyJzdInen{H17\3),;]83/0=/_+tVa0\[)**3+.+)l+;8Z0*),)\k`8.0,/385/8;+,/`
/_30\;_8,),/38l+,0\5$6'.8/_+/+3$5k8**/\^+'b/[)**^+Z0,+=03^0/_3+5+83._8,Zk+Z).8*
]\3]05+5b
/)5,0/r,0[,)=tV[)/_.0,/385/)5.0k]*+/+*a58=+=038Z+l+*0]),;=+/\5bT_+3+=03+`8**
[0k+,0=._)*Z^+83),;]0/+,/)8*[)**_8l+8]3+;,8,.a/+5/]+3=03k+Z,0k03+/_8, %_0\35
^+=03++8._tV5.8,[)/_.0,/385/bT_+5.8,[)**,0/^+Z0,+)=/_+]3+;,8,.a/+5/)5]05)/)l+b
|}~~}
|}~}


	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<0=>?
@@BCDC@EF@GHDIBJKHDLMNOMPNMOP
Q	R	

4S-TUUS2VWU42VUTV4X4VXWT-TVW4-UY7Z
U[+3)5\50=8,6.8/[+/+3),.*]^+_*++^),;`),=+./)0,`03),=*8aa8/)0,0=/[+5\),8,^b+),c)/[
d8),8,^5c+**),;e
f)*^5gad/0a5=30a;8^0*),)]a),=]5)0,0..]3),=+c+3/[8,&h0=/[05+c[03+.+)b+)/8,^
]5]8**g;08c8gi]).\*gef)*^5gad/0a5a8g),.*]^+.0*^,+55),/[+83a^]3),;/[+),j+./)0,`8
a+/8**)./85/+`[+8^8.[+`8,^,8]5+8e,8,+k/3+a+*g5a8**,]a_+3`=+c+3/[8,0,+),l99`999
d+0d*+`a03+5+b+3+5gad/0a5[8b+_++,3+d03/+^),.*]^),;5[03/,+550=_3+8/[`c[++m),;`
[)b+5`8,^*0c+3),;0=_*00^d3+55]3+eZ0]5[0]*^,0/3+.+)b+;8^0*),)]a)=g0]d3+b)0]5*g[8^
8,8**+3;).3+8./)0,/0)/eZ0]c)**_+85\+^8_0]/5].[8**+3;).3+8./)0,5_+=03+;8^0*),)]a)5
;)b+,e
2+0d*+c)/[\)^,+g^)5+85+83+8/3)5\=0385+3)0]53+8./)0,/0;8^0*),)]a.0,/385/.8**+^
n,+d[30;+,).5g5/+a).=)_305)5$-('oeU[)5.0,^)/)0,8*c8g5),b0*b+5/[+5\),8,^.8,8*50
),b0*b+/[+a]5.*+5`j0),/58,^),/+3,8*03;8,5e-([853+5]*/+^),8b+3g5a8**,]a_+30=
^+8/[5eV_*00^/+5/0=g0]3\)^,+g=],./)0,a8g_+^0,+c)/[),/[+a0,/[_+=03+8,fW5.8,
c)/[;8^0*),)]a.0,/385/eZ0]c)**,0/3+.+)b+;8^0*),)]a=0383+5+83.[fW5.8,)=g0]3
\)^,+g=],./)0,)5_+*0c/[+58=+*+b+*e
f05/0=/[+;8^0*),)]a.0,/385/*+8b+5/[+_0^g),/[+]3),+e0c+b+3`/[+(7V[85)55]+^8
58=+/g8*+3//[8/),^).8/+55a8**8a0],/50=;8^0*),)]aa8g3+a8),),/[+_0^g=03a0,/[5/0
g+835eU[++==+./50=/[+3+/8),+^;8^0*),)]a83+,0/.*+83eV//[)5/)a+`3+/8),+^;8^0*),)]a[85
,0/_++,*),\+^/0[+8*/[3)5\5),d+0d*+c[05+\)^,+g5c03\c+**e-0a+/gd+50=;8^0*),)]a
.0,/385/^3];583+*+55*)\+*g/03+a8),),/[+_0^g/[8,0/[+35e,/[)55/]^g`c+c)**]5+/[+
;8^0*),)]a.0,/385/^3];5/[8/83+*+55*)\+*g/03+a8),),/[+_0^ge
pqrrstuv
7]3),;g0]3d83/).)d8/)0,),/[)53+5+83.[5/]^g`g0]a8g_++kd05+^/038^)8/)0,=30a]d/0%
4U5.8,5+8.[g+83eU[+8a0],/0=38^)8/)0,+kd05]3+=30a/[+5+d30.+^]3+5)5+i]8*/0
8dd30k)a8/+*gwe 3+aeV3+a)58],)/0=8_503_+^38^)8/)0,e
Tb+3g^8g`d+0d*+83++kd05+^/0*0c*+b+*50=38^)8/)0,/[8/.0a+=30a/[+5],8,^/[+
+,b)30,a+,/830],^/[+aeU[+8b+38;+d+350,),/[+Y,)/+^-/8/+53+.+)b+5838^)8/)0,+kd05]3+
0=9el3+ad+3g+83=30a/[+5+50]3.+5eU[)5/gd+0=38^)8/)0,)5.8**+^n_8.\;30],^38^)8/)0,eo
U[)55/]^gc)**+kd05+g0]/0a03+38^)8/)0,/[8,g0];+/=30a+b+3g^8g_8.\;30],^38^)8/)0,e
00,+\,0c5=035]3+c[+/[+3+kd05]3+/0/[+5+*0c8a0],/50=38^)8/)0,)5[83a=]*/0g0]3
_0^ge
U[+4U5.8,5/[8/g0];+/),/[)55/]^gc)**+kd05+g0]/0/[+30];[*g/[+58a+8a0],/0=
38^)8/)0,85&"elg+835xc03/[0=_8.\;30],^38^)8/)0,ey+),;+kd05+^/0/00a].[38^)8/)0,.8,
.8]5+[83a=]*5)^++==+./55].[858,),.3+85+),/[+3)5\0=.8,.+3eU[+3)5\^+d+,^50,[0c
a].[38^)8/)0,g0]83++kd05+^/0e2*+85+_+8c83+/[8/8_0]/%90]/0=&99d+0d*+$%9h'c)**
;+/.8,.+3^]3),;/[+)3*)=+/)a+`8,^ 90]/0=&99$ 9h'c)**^)+=30a.8,.+3eU[+3)5\0=;+//),;
.8,.+3=30a/[+38^)8/)0,+kd05]3+),/[)55/]^g)59ew0]/0=&99$9ewh'8,^0=;+//),;8=8/8*
.8,.+3)59el0]/0=&99$9elh'e
z{|~|{
z{|{


	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<0=>?
@@BCDC@EF@GHDIBJKHDLMNOMPNMOP
Q	R	

4S-TUUS2VWU42VUTV4X4VXWT-TVW4-UY7Z
Z0[\8],0/^83/).)^8/+),/_)55/[`])=]0[83+^3+;,8,/a=]0[83+8b*+/0b+.0\+^3+;,8,/cd+
d)**^+3=03\8^3+;,8,.]/+5/b+=03++e^05),;]0[/038`)8/)0,aZ0[\[5//+**[5)=]0[\8]_8f+
b+.0\+^3+;,8,/d)/_),/_+^3+f)0[5&%`8]5b+.8[5+/_+^3+;,8,.]/+5/)5[,3+*)8b*+`[3),;/_8/
/)\+a
gCDIDhijKHiklmn
ojDHKIilkBpDlqrsJDlkJtn5)`++==+./583++e/3+\+*][,*)u+*]aW+;[*83[5+0=8.+/8\),0^_+,.8,
.8[5+*)f+3`8\8;++5^+.)8**]8/_);_`05+5$\03+/_8,%999\;:`8]03& 3+;[*835/3+,;/_/8b*+/5
^+3`8]'aU0\),)\)v+/_)5^055)b)*)/]c]0[5_0[*`,0//8u+0f+3/_+.0[,/+3^30`[./5.0,/8),),;
8.+/8\),0^_+,8,`5_0[*`8f0)`8*.0_0*`[3),;/_+/)\+^+3)0`5]0[83+/8u),;5._+`[*+`
8.+/8\),0^_+,`05+50,/_)55/[`]a
EKliHihilDqwKlHKjtn^055)b*+5)`++==+./5),.*[`+/)3+`,+55c`)vv),+55c_+8`8._+c8,``)833_+8a
xshCkyszilDqoHKCKytn2055)b*+5)`++==+./5),.*[`+5*++^),+55c`)vv),+55c3+5/*+55,+55c8,`
)33)/8b)*)/]a
{DyKIDHpKmklDq{DjKhCkltnU0b+;)f+,0,*]b+=03+/_+=)35/0=/_+|`05+5c0,*]=033+/3+8/\+,/
$.].*+5 }'.].*+5a-)`++==+./50=85),;*+`05+0=`+e8\+/_850,+\);_/),.*[`+`)==).[*/]
5*++^),;c),.3+85+`_[,;+3c8,`),.3+85+`b*00`;*[.05+a-/+30)`583+8550.)8/+`d)/_8,),.3+85+`
3)5u0=),=+./)0,8*/_0[;_/_)5)5\03+.0\\0,d_+,/_+]83+[5+`\03+=3+~[+,/*]a
28/)+,/5),=+./+`d)/_6d)**b++e.*[`+`=30\/_)5/3)8*b+.8[5+/_++==+./0=X 0,6
3+^*).8/)0,8,`:03/_+)\\[,+5]5/+\)5[,u,0d,8,`^0/+,/)8**]_83\=[*a28/)+,/5d)/__+^8/)/)5
45[3=8.+8,/);+,^05)/)f)/]83++e.*[`+`=30\/_)5/3)8*b+.8[5+/_++==+./0=X 0,_+^8/)/)5
48,`:03/_+)\\[,+5]5/+\)5[,u,0d,8,`^0/+,/)8**]_83\=[*a28/)+,/5/_8/83+^3+;,8,/03
b3+85/=++`),;d)**b++e.*[`+`=30\/_)5/3)8*b+.8[5+/_++==+./0=X 0,8`+f+*0^),;=+/[5
038,[35),;
),=8,/
)5
[,u,0d,
8,`
^0/+,/)8**]
_83\=[*a

28/)+,/5
d)/_
._)*`b+83),;
^0/+,/)8*
5_0[*`
[5+8`+~[8/+b)3/_.0,/30*\+85[3+5d_)*+0,/_+5/[`]c[,/)*|9`8]58=/+3/_+*85/`05+0=X
 a
+d)**.83+=[**]\0,)/03]0[/0`+/+./8,]0=/_+5+5)`++==+./5),8``)/)0,c]0[d)**b+/8[;_/
8b0[/5)`++==+./5cd_)._]0[\8]+e^+3)+,.+8,`\[5/3+^03/)\\+`)8/+*]aV*/_0[;_5)`++==+./5
0=/_)5/3+8/\+,/[5[8**]*85/=0385_03/^+3)0`0=/)\+8,`.0\^*+/+*]3+50*f+c]0[\8]+e^+3)+,.+
5)`++==+./5/_8/83+^+3\8,+,/aV*/_0[;_,0/+e^+./+`c`+8/_.0[*`0..[3=30\/_)5+e^+3)\+,/8*
/3+8/\+,/a/)5f+3])\^03/8,//_8/]0[,0/)=][585500,85^055)b*+)=]0[+e^+3)+,.+8,]/]^+0=
5)`++==+./50/_8/]0[.8,b+.83+=[**]+e8\),+`aV**^3+.8[/)0,5d)**b+/8u+,/0^3+f+,//_+5+
5)`++==+./58,`]0[d)**b+/3+8/+`^30\^/*]$)=/3+8/\+,/)53+~[)3+`8,`^055)b*+')=/_+]0..[3a
U3+8/\+,/0,/_)55/[`]d)**3+~[)3+85);,)=).8,/8\0[,/0=]0[3/)\+8,`\8]b+5/3+55=[*a
283/).)^8/),;),/_)55/[`]\8]^3+f+,/]0[=30\b+),;),0/_+33+5+83._5/[`)+5),/_+=[/[3+a

_)*+d+_0^+/_8/X /3+8/\+,/d)**b+b+,+=).)8*/0]0[cd+`0,0/u,0d)=]0[d)**3+.+)f+
^+350,8*c\+`).8*b+,+=)/=30\/_)5/3+8/\+,/aX /3+8/\+,/\8].8[5+)\^30f+\+,/),]0[3
*+[u+\)85[._853+`[./)0,),.8,.+33+*8/+`5]\^/0\5aZ0[3^83/).)^8/)0,),/_)55/[`]\8]_+*^
[58`f8,.+/_+[,`+35/8,`),;0=/_+[5+0=b)0*0;).8;+,/5),/_+/3+8/\+,/0=4Xa




	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<0=>?
@@BCDC@EF@GHDIBJKHDLMNOMPNMOP
Q	R	

4S-TUUS2VWU42VUTV4X4VXWT-TVW4-UY7Z
[\
Z0]^8_`+.)`+,0a,0//03+.+)b+/3+8/^+,/),/c)5d30/0.0*03_0]^8_.c005+8/8,_d0),/),
/)^+/05/0d/c+`3];8,`a)/c`38a=30^/c+d30/0.0*e,+)/c+3.85+_0]a0]*`f+3+/]3,+`/0/c+
.83+0=_0]33+=+33),;dc_5).)8,e
g+.8]5+0=/c+/_d+8,`+h/+,/0=_0]3/]^03i.c+^0/c+38d_)5=+*//0f+^03+f+,+=).)8*/c8,
5]3;+3_0338`)8/)0,8*0,+eV*/+3,8/)b+8dd308.c+5/c8/.0]*`f+]5+`^8_),.*]`+1
&e
S/c+3=03^50=/3+8/^+,/1
8e
-+b+38*`3];5f+5)`+5.*8`3)f),+iac).c_0]c8b+8*3+8`_f++,/3+8/+`a)/ci.8,f+
]5+=]*),d8/)+,/5a)/c4XeW)/]h)^8f)58^0,0.*0,8*8,/)f0`_ac).cc85f++,3+d03/+`
/0d30`].+3+5d0,5+5),d8/)+,/5a)/c4Xe2+,/05/8/),i=*]`838f),+8,`),/+3=+30,83+8*50
`3];5ac).c.8,d30`].+3+5d0,5+5),4Xif]//c+_8*50.8,c]3//c+)^^],+5_5/+^e
fe
W8`)8/)0,/3+8/^+,/iac).c50^+/)^+5.8,.0,/30*/]^03;30a/c),*0.8*83+855].c
85*_^dc
,0`+5i
5d*++,
8,`
f0,+5e

0a+b+3i
/c)5
8dd308.c
a)**
,0/
+==+./)b+*_
/3+8/
`)5+85+
/c8/c855d3+8`f+_0,`/c+83+85/c8/83+)338`)8/+`e
.e
-]3;+3_iac).c.8,f+]5+`/03+^0b+/c+5d*++,$)=/c)5c85,0/8*3+8`_f++,`0,+'e
 e
S/c+3+hd+3)^+,/8*8;+,/5e
je
k+//),;,0/3+8/^+,/l;+//),;.0^=03/.83+i8*50.8**+`d8**)8/)b+.83+eUc)5/_d+0=.83+
c+*d53+`].+d8),i/)3+`,+55i8dd+/)/+d30f*+^5i8,`0/c+3d30f*+^5.8]5+`f_.8,.+3e/`0+5,0/
/3+8//c+.8,.+3`)3+./*_if]/),5/+8`/3)+5/0)^d30b+c0a_0]=++*e40^=03/.83+/3)+5/0m++d_0]
858./)b+8,`.0^=03/8f*+85d055)f*+e
n
opq\	\r
S,.+_0].0^d*+/+/c+5/]`_/3+8/^+,/i_0]^8_c8b+d+3)0`).b)5)/5+b+3_j& ^0,/c58//c+
4*),).8*4+,/+3038/_0]3*0.8*d30b)`+3=03=0**0a]d+h8^),8/)0,8,`/+5/5$)e+ef*00`/+5/5
8,`)^8;),;'eUc+^8h)^]^8^0],/0=f*00`a+^8_`38a=033+5+83.c),+8.c=0**0a]db)5)/
)5 /+85d00,5e=/c+`)5+85+a035+,5/c+,_0]^8_,++`0/c+3/c+38d_eV//c8//)^+_0]a)**f+
;)b+,/c+0dd03/],)/_0=d83/).)d8/),;),8``)/)0,8*3+5+83.cd30/0.0*5/c8/^8_f+8dd30d3)8/+=03
_0]e=,05].cd30/0.0*583+8b8)*8f*+i_0]a)**f+3+/]3,+`/0/c+.83+0=_0]3*0.8*dc_5).)8,e
/)5)^d03/8,//05/3+55/c8/d83/).)d8/)0,),/c)5d30/0.0*`0+5,0/.0,5/)/]/+8d30^)5+0=*0,;
/+3^^+`).8*.83+c+3+8//c+4*),).8*4+,/+3e=/c+3+)5,03+5+83.c5/]`_/c8/)55])/8f*+=03_0]
8,`_0]35/8;+0=`)5+85+i_0]a)**f+3+/]3,+`/0/c+.83+0=_0]3d3)b8/+`0./0303/08.*),).),
_0]3*0.8*.0^^],)/_e/)5.0,.+)b8f*+/c8/d83/).)d8/)0,),/c)55/]`_^8_^8m+_0]),+*);)f*+
/0d83/).)d8/+),.+3/8),0/c+33+5+83.cd30/0.0*5f+.8]5+/c+3+s])3+^+,/5=03+,/3_0,/0/c+5+
d30/0.0*5^8_`)58**0ad8/)+,/5ac0c8b+8*3+8`_f++,/3+8/+`a)/c.+3/8),`3];503ac0c8b+
c8`.+3/8),5)`++==+./5=30^d3+b)0]5/3+8/^+,/eZ0]^8_`+.)`+,0a,0//03+.+)b+/3+8/^+,/
0,/c)5d30/0.0*i03_0]^8_.c005+8/8,_d0),/),/)^+/05/0d/c+/3+8/^+,/8,`a)/c`38a=30^
/c+d30/0.0*l),+)/c+3.85+_0]a)**f+3+/]3,+`/0/c+.83+0=_0]33+=+33),;dc_5).)8,e
	qq
tuvxyzv{u|}~}}
tuvu{|}~}


	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<=0><?
@@BCDC@EF@GHDIBJKHDLMNOMPNMOP
Q	R	

4S-TUUS2VWU42VUTV4X4VXWT-TVW4-UY7Z
U[+8/)0,8*,5/)/\/+50>+8*/[$'3+])+^55/8>>3+5+83.[+358/*+85/_+83*_>03.0,>*)./5
0>),/+3+5/`U[)5a30.+55)5b+/8)*+b),8230/0.0*W+])+^c\)b+`Z0\d8_85e_0\33+5+83.[/+8d
>038.0a_0>/[+230/0.0*W+])+^c\)b+03>03d03+),>03d8/)0,`f+dg+350>/[+3+5+83.[/+8d
^[0b0,0/^03e>0383++ha+./+b/0>0**0^/[+5+;\)b+*),+5g\//[+_b0,0/,++b/03+a03/
/[+)3a+350,8*>),8,.+5/0/[+`
U[+8/)0,8*,5/)/\/+50>+8*/[8,b/[+3+5+83.[/+8d>03/[)55/\b_[8]+b+]+*0a+b8b3\;ig+),;
\5+b),/[)55/\b_`U[)5d+8,5)/)5a055)g*+/[8//[+3+5\*/50>/[)55/\b_.0\*b*+8b/0a8_d+,/5/0
5.)+,/)5/5
8,b
/0
/[+
`
j_
*8^i
;0]+3,d+,/
5.)+,/)5/5
83+
3+k\)3+b
/0
3+.+)]+
5\.[
a8_d+,/5
>03/[+)3),]+,/)0,5`Z0\^)**,0/3+.+)]+8,_d0,+_>30d/[+b+]+*0ad+,/0>Xfj `
lmnm
U08b]8,.+5.)+,.+i)/)5[+*a>\*>033+5+83.[+35/05[83+),>03d8/)0,/[+_;+/>30d5/\b_),;[\d8,
58da*+5`U[+_b0/[)5g_a\//),;)/),/00,+03d03+5.)+,/)>).b8/8g85+5i^[+3+)/)55/03+b8*0,;
^)/[),>03d8/)0,>30d0/[+35/\b)+5`V3+5+83.[+3^[0^8,/5/05/\b_/[+),>03d8/)0,d\5/8aa*_
/0/[+b8/8g85+8,bg+8aa30]+b`W+5+83.[+35\5+5a+.)d+,58,bb8/85/03+b),5.)+,/)>).b8/8g85+5
/08b]8,.+5.)+,.+8,b*+83,8g0\/[+8*/[8,bb)5+85+`
o+a*8,/0e++a50d+0>/[+5a+.)d+,58,bb8/8/[8/^+.0**+./i\5+/[+d>03>\/\3+3+5+83.[8,b
5[83+/[+d^)/[0/[+33+5+83.[+35`o+^)**,0/.0,/8./_0\/085e8g0\/+8.[0>/[+5+>\/\3+\5+5`
U[+5+5a+.)d+,58,bb8/8^)**g+5/3)aa+b0>)b+,/)>)+355\.[85,8d+i8bb3+55038..0\,/,\dg+3i
50/[8//[+_d8_g+\5+b>03>\/\3+3+5+83.[0,8,_/0a).8,b5[83+bg308b*_>033+5+83.[a\3a05+5`
Z0\35a+.)d+,58,bb8/8^)**g+\5+b>033+5+83.[a\3a05+50,*_8,b^)**,0/g+,+>)/_0\`/)5
8*50a055)g*+/[8//[+5/03+b5a+.)d+,58,bb8/8d8_,+]+3g+\5+b`W+5\*/50>3+5+83.[b0,+0,
_0\35a+.)d+,58,bb8/8^)**,0/g+8]8)*8g*+/0_0\03_0\3b0./03`/d);[/[+*aa+0a*+^[0[8]+
.8,.+38,b0/[+3b)5+85+5),/[+>\/\3+`
>_0\b0,0/^8,/_0\35/03+b5a+.)d+,58,bb8/8\5+b>03>\/\3+3+5+83.[ia*+85+.0,/8./\5),
^3)/),;8,b*+/\5e,0^/[8/_0\b0,0/^8,/\5/0\5+_0\35a+.)d+,58,b:03b8/8`U[+,8,_/[8/
[8]+,0/8*3+8b_g++,\5+b035[83+b^)**g+b+5/30_+b8,b_0\3b8/8^)**,0/g+\5+b>03>\/\3+
3+5+83.[`0^+]+3i)/d8_,0/g+a055)g*+/0^)/[b38^03b+*+/+d8/+3)8*503b8/80,.+/[+_[8]+
g++,5[83+b^)/[0/[+33+5+83.[+35`
-8da*+5/0g+58]+b>038bb)/)0,8*/+5/51
p +\/38*)q),;8,/)g0b)+51V,/)g0b)+58a8/)+,/d);[/d8e+^[).[g*0.e/[++>>+./0>.+3/8),
3+.0dg),8,/)dd\,0/0h),5*)e+Xfj `W+k\)3+58g0\/&/+85a00,0>g*00b`
p (*0^._/0d+/3_8558_5/0k\8,/)>_/\d03d83e+350,/[+d8*);,8,/.+**5`W+k\)3+58g0\/
&: /+85a00,0>g*00b`
p j0,+d8330^g)0a5_58da*+5i^[+/[+3/[+_0g/8),+b8/03+*5+^[+3+i8,b^[+/[+3/[+
g0,+d8330^/+5/[858*3+8b_g++,b0,+03,0/_+/b0,+`
p 4_/0/0h).)/_8558_5`X+\e+d)8.+**5>30d/[+g*00big0,+d8330^i030/[+3/)55\+5d8_g+
/+5/+b^)/[Xfj 8,b3+*8/+bb3\;5/0b+/+3d),+)>/[+d8*);,8,/.+**5.8,g+e)**+b
0\/5)b+/[+g0b_`W+k\)3+5&r/8g*+5a00,50>g*00b`
stuwxyuzt{|}~||
stutz{|}|


	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<<0=<>
??ABCB?DE?FGCHAIJGCKLMNLOMLNO
P	Q	

4R-STTR2UVT42UTSU4W4UWVS-SUV4-TX7Y
Z -0*[\*+47 ]^47  ^8,_0/`+3/[a03a83b+351T0+5/)a8/+/`+8a0[,/0=.8,.+3.+**5),
/`+\0_c\ca+85[3),;d30/+),5e`).`=8**0==.8,.+3.+**58,_;0),/0/`+\*00_fV+g[)3+5
8\0[/&/+85d00,0=\*00_f
Z WU/cd),;/0\+//+3[,_+35/8,_/`+)aa[,+5c5/+a),d8/)+,/5e)/``8)3c.+***+[b+a)8f
V+g[)3+58\0[/&/+85d00,0=\*00_f
Z 2Uh;+,+/[\+1T00\/8),VU/05/[_c/`+a+.`8,)5a0=`0e*+[b+a)8.+**5=03a^8,_
/0_+/+./i+3c*0e*+i+*50=*+[b+a)8.+**5),d8/)+,/5fV+g[)3+58\0[/&: /+85d00,0=
\*00_f
Z VU58ad*+5.8,8*50\+[5+_^),8,8558c.8**+_a).308338c5^/05/[_ce`c50a+d8/)+,/5
a8c,0/3+5d0,_85e+**850/`+35/03+.0a\),8,/)aa[,0/0j),5*)b+Wkl fT8b+,e)/`
28jm+,+/[\+f
Z -8ad*+50=\*00_/05/[_c`0e`+a0*c/).[3+a).5c,_30a+$X-'^8a8n03/0j).)/c0=8
3+.0a\),8,/)aa[,0/0j),.8**+_lW  ^e`).`)55)a)*83/0Wkl ^0..[358,_a);`/\+
d3+i+,/+_fV+g[)3+58\0[/&: /+85d00,0=\*00_f
Z 7U58ad*+5/0*00b=038\,03a8*)/)+5e`).`a);`/a8b+8d8/)+,/a03+5[5.+d/)\*+/0
X-fV+g[)3+58\0[/&: /+85d00,0=\*00_f
Z U558c5e`).`.0[*_`8i+8,)ad8./0,\0/`d8/)+,/58,_/`+)3.`)*_3+,^),.*[_),;5/[_)+5
0=;+,+/)..8,.+33)5b^e)**,0/\+_0,+f
Z -8ad*+5/0_+/+3a),+*+i+*50=)aa[,0/0j),),\*00_^[3),+^8,_0/`+3/)55[+5f
Y0[33+5+83.`\*00_58ad*+5e)**0,*c\+)_+,/)=)+_\c/`+5/[_c.0_+^5[\n+./,[a\+3^i)5)/
,[a\+38,__8/+8,_/)a+0=.0**+./)0,f
oP
pqrsrtuv
Y0[e)**,0/3+.+)i+8,cd8ca+,/=03/8b),;d83/),/`)55/[_cf
PwxP
pqsyssyzrv
283/).)d8,/5e)**\+3+)a\[35+_=03`0/+*^/38i+*^a+8*5$;0i+3,a+,/38/+'fV+.+)d/5a[5/\+
d30i)_+_=038**+jd+,5+5f
R,/`)55/[_c^/`+4e)**3+)a\[35+/`+.05/=0350a+0=c0[3+jd+,5+55[.`85/`05+=03`0/+*^
/38i+*^a+8*5f-0a+0=/`+5+.05/5a8c\+d8)__)3+./*c\c/`+8,_50a+a8c\+3+)a\[35+_
8=/+3c0[`8i+d8)_fT`+8a0[,/8,_=03a0=/`+5+d8ca+,/583+_+/+3a),+_\c/`+4T38i+*
8,_W0_;),;V+)a\[35+a+,/20*).cfY0[e)**\+;)i+,85[aa83c0=/`+d0*).ce`).`d30i)_+5
a03+),=03a8/)0,f
=c0[3/38i+*/0/`+4*),).8*4+,/+3$+f;f=*);`/^`0/+*')58338,;+_8,_d8)_=03\c/`+^
/`+8;+,.ca8b),;/`+3+5+3i8/)0,58,_/`+)33+d3+5+,/8/)i+5e)**`8i+8..+55/0c0[3)_+,/)=)8\*+
),=03a8/)0,f
{|}}|
{|}|


	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<=0><?
@@BCDC@EF@GHDIBJKHDLMNOMPNMOP
Q	R	

4S-TUUS2VWU42VUTV4X4VXWT-TVW4-UY7Z
	

[\]]^
_0+5,0/`)**a+8*/a),5b38,.+.0cd8,)+503d83/).)d8,/5>038,e3+5+83.a033+*8/+_.*),).8*
.83+/a8/e0b3+.+)f+8//a+4*),).8*4+,/+3g
h >50c+/+5/58,_d30.+_b3+583+d+3>03c+_0b/5)_+/a+4*),).8*4+,/+3ie0bc8ea8f+
/0d8e>03/a+5+.05/5)>/a+e83+,0/.0f+3+_`ee0b3),5b38,.+.0cd8,eg
h j+_).),+5/a8/83+,0/d83/0>/a+5/b_e/3+8/c+,/k)**,0/`+d30f)_+_03d8)_>03`e/a+
4*),).8*4+,/+3g
h S,.+e0ba8f+.0cd*+/+_/8l),;d83/),/a+5/b_eic+_).8*.83+k)**,0*0,;+3`+d30f)_+_
`e/a+4*),).8*4+,/+3g
	R	RRm
nR
m
V_+5.3)d/)0,0>/a)5.*),).8*/3)8*k)**`+8f8)*8`*+0,a//d1::kkkg4*),).8*U3)8*5g;0fi853+ob)3+_
`eYg-gX8kgUa)5p+`5)/+k)**,0/),.*b_+),>03c8/)0,/a8/.8,)_+,/)>ee0bgV/c05/i/a+p+`
5)/+k)**),.*b_+85bcc83e0>/a+3+5b*/5gZ0b.8,5+83.a/a)5p+`5)/+8/8,e/)c+g
	
Rq
	

rsnq
[tutv\w^
p+k)**_00b3`+5//0c8l+5b3+/a8//a+d+350,8*),>03c8/)0,),e0b3c+_).8*3+.03_k)**`+l+d/
d3)f8/+g0k+f+3ik+.8,,0/;b838,/++/0/8*d3)f8.egS3;8,)x8/)0,5/a8/c8e*00l8/8,_:03.0de
e0b3c+_).8*3+.03_5>033+5+83.aiob8*)/e855b38,.+i8,__8/88,8*e5)5),.*b_+1
h Ua+8,_0/a+3;0f+3,c+,/8;+,.)+5i*)l+/a+(00_8,_73b;V_c),)5/38/)0,$(7V'i
ka).a83+),f0*f+_),l++d),;3+5+83.a58>+>03d+0d*+g
h 8/)0,8*,5/)/b/+50>+8*/a,/38cb38*,5/)/b/)0,8*W+f)+ky083_
Ua+3+5+83.a+35.0,_b./),;/a)55/b_e8,_/a+>0**0k8dd*).8`*+*8k58,_d0*).)+5/0l++d
e0b3)_+,/)>e),;),>03c8/)0,d3)f8/+/0/a++z/+,/d055)`*+g0k+f+3i/a+3+)58*k8e58.a8,.+
/a8/i_+5d)/+0b3`+5/+>>03/5ie0b3)_+,/)/e8,_:03),>03c8/)0,8`0b/e0b3d83/).)d8/)0,),/a)5
3+5+83.ac8e`+),8_f+3/+,/*e3+*+85+_03)cd30d+3*e8..+55+_`eb,8b/a03)x+_d+350,5g
,c05/.85+5i/a+k)**,0/3+*+85+8,e)_+,/)>)8`*+),>03c8/)0,.0**+./+_8`0b/e0bk)/a0b/
e0b3k3)//+,d+3c)55)0,g0k+f+3ie0b3),>03c8/)0,c8e`+5a83+_85_+5.3)`+_),/a+5+./)0,0>
/a)5_0.bc+,/0,5a83),;0>5d+.)c+,58,__8/8i8,_85>b3/a+30b/*),+_),/a+>0**0k),;5+./)0,5g
(b3/a+3i/a+),>03c8/)0,.0**+./+_>03/a)55/b_e)5d30/+./+_`eb,_+384+3/)>).8/+0>
40,>)_+,/)8*)/e8,_/a+23)f8.eV./g
	uu	u
U0a+*db5d30/+./e0b3d3)f8.ei/a+,/38cb38*230;38ca853+.+)f+_84+3/)>).8/+0>
40,>)_+,/)8*)/e$4+3/)>).8/+'gp)/a/a)5.+3/)>).8/+i3+5+83.a+35c8e,0/3+*+85+03b5+_8/803
),>03c8/)0,8`0b/e0b+z.+d/),.+3/8),.)3.bc5/8,.+5g
{|}}|
{|}|


	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<=0><?
@@BCDC@EF@GHDIBJKHDLMNOMPNMOP
Q	R	

4S-TUUS2VWU42VUTV4X4VXWT-TVW4-UY7Z
3+5+83.[+35\]5/,0/5[83+),>03\8/)0,/[8/\8^)_+,/)>^^0]),8,^>+_+38*`5/8/+`03*0.8*
.)a)*`.3)\),8*`8_\),)5/38/)a+`*+;)5*8/)a+`030/[+3b30.++_),;5`>03+c8\b*+`)>3+d]+5/+_e^8
.0]3/f
U[+4+3/)>).8/+_0+5,0/b30/+./^0]3),>03\8/)0,g[+,)/1
&f)5_)5.*05+_/0b+0b*+.0,,+./+_g)/[/[+3+5+83.[`>03+c8\b*+`),>03\8/)0,\8^e+]5+_
>038]_)/),;03b30;38\+a8*]8/)0,),/+3,8**^e^/[+h03
 f)53+d])3+_/0e+_)5.*05+_e^(+_+38*`-/8/+`03*0.8**8g5`>03+c8\b*+`g[+,),>03\8/)0,
\]5/e+_)5.*05+_/0\++//[+*+;8*3+d])3+\+,/50>/[+>+_+38*(00_8,_73];
V_\),)5/38/)0,$(7V'h
if)5>030/[+33+5+83.[h
%f)5_)5.*05+_g)/[^0]3.0,5+,/f
U[+4+3/)>).8/+_0+5,0/b3+a+,/^0]>30\a0*],/83)*^3+*+85),;),>03\8/)0,8e0]/^0]35+*>03^0]3
),a0*a+\+,/),/[)53+5+83.[f
U[+4+3/)>).8/+g)**,0/e+]5+_/0b3+a+,/_)5.*05]3+/05/8/+03*0.8*8]/[03)/)+50>[83\/05+*>03
0/[+35),.*]_),;`>03+c8\b*+`.[)*_8e]5+8,_,+;*+./`8,_e^5);,),;e+*0g^0].0,5+,//0/[05+
_)5.*05]3+5fS/[+3b+3\)55)0,5>033+*+85+\8^e+\8_+e^5);,),;>03\5`5].[85/[+0/).+
8,_V.j,0g*+_;+\+,/0>,>03\8/)0,238./).+5.0,5+,/f
k
U[+(+_+38*23)a8.^V./;+,+38**^b30/+./5/[+.0,>)_+,/)8*)/^0>^0]3\+_).8*3+.03_5g+
.0**+./],_+3/[+8]/[03)/^0>/[+2]e*).+8*/[-+3a).+V./f,50\+.85+5`/[+23)a8.^V./
b30/+./)0,5_)>>+3>30\/[+4+3/)>).8/+0>40,>)_+,/)8*)/^f(03+c8\b*+`50\+/)\+5/[+23)a8.^V./
8**0g53+*+85+0>),>03\8/)0,>30\^0]3\+_).8*3+.03_g)/[0]/^0]3b+3\)55)0,`>03+c8\b*+`)>
)/)53+d]+5/+_e^40,;3+55f,>03\8/)0,\8^8*50e+3+*+85+_>03.+3/8),3+5+83.[b]3b05+5g)/[
_]+.0,5)_+38/)0,8,_b30/+./)0,`/0/[05++,;8;+_e^/[+8;+,.^>033+5+83.[b]3b05+5`/0.+3/8),
>+_+38*8,_5/8/+8;+,.)+5`>036b83/,+3,0/)>).8/)0,`>03),>+./)0]5_)5+85+038e]5+03,+;*+./
3+b03/),;`/0/]\033+;)5/3)+5`>03d]8*)/^855+55\+,/8,_\+_).8*8]_)/5`03g[+,/[+)5
),a0*a+_),8*8g5])/f0g+a+3`g)**0,*^3+*+85+),>03\8/)0,>30\^0]3\+_).8*3+.03_)>)/
)5b+3\)//+_e^e0/[/[+4+3/)>).8/+0>40,>)_+,/)8*)/^8,_/[+23)a8.^V./f

R	lmm	noRpq
U[+4*),).8*4+,/+3g)**b30a)_+5[03//+3\\+_).8*.83+>038,^),r]3^3+5]*/),;>30\^0]3
b83/).)b8/)0,),3+5+83.[[+3+f,;+,+38*`,0*0,;/+3\\+_).8*.83+03>),8,.)8*.0\b+,58/)0,>03
3+5+83.[3+*8/+_),r]3)+5g)**e+b30a)_+_e^/[+`/[+4*),).8*4+,/+3`03/[+(+_+38*
s0a+3,\+,/f0g+a+3`^0][8a+/[+3);[//0b]35]+*+;8*3+\+_^)>^0]e+*)+a+/[8/^0]3),r]3^
r]5/)>)+55].[8./)0,f

tRQ
uq

>^0][8a+8,^b30e*+\503d]+5/)0,58e0]//[)55/]_^`038e0]/^0]33);[/58583+5+83.[
b83/).)b8,/`038e0]/8,^3+5+83.[3+*8/+_),r]3^`.0,/8.//[+23),.)b8*,a+5/);8/03`W0e+3/
v3+)/\8,`j3+)/\83w\8)*f,)[f;0a`8/i9&%x9y&x"fZ0]\8^8*50.8**/[+4*),).8*4+,/+3
28/)+,/W+b3+5+,/8/)a+8/i9&%!y y y`03/[+S>>).+0>WzSb+38/)0,58/i9&%9 i"&i`)>
^0][8a+83+5+83.[3+*8/+_.0\b*8),/03.0,.+3,f
{|}}|
{|}|


	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<=0><?
@@BCDC@EF@GHDIBJKHDLMNOMPNMOP
Q	R	

4S-TUUS2VWU42VUTV4X4VXWT-TVW4-UY7Z
	

	[Q
2*+85+\++]8.0]^0>/_)5`0.ab+,/),.85+^0ac8,//03+8`)/8;8),d
efgijkglfmnopnqrn
efgsttfuvswxsylmnz{qo{|n|q


	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<=0><?
@@BCDC@EF@GHDIBJKHDLMNOMPNMOP
Q	R	

4S-TUUS2VWU42VUTV4X4VXWT-TVW4-UY7Z
[\8]+3+8^/\++_`*8,8/)0,8a0b//\)55/b^c8,^\8]+a++,;)]+,/\+0``03/b,)/c
/0^)5.b55)/8,^/085deb+5/)0,5f.0,5+,//0`83/).)`8/+),/\)55/b^cf
-);,8/b3+0>W+5+83.\283/).)`8,/
23),/8g+0>W+5+83.\283/).)`8,/
78/+
RhijkRlmno	[\8]+3+8^/\++_`*8,8/)0,
8a0b//\)55/b^c8,^\8]+a++,;)]+,/\+0``03/b,)/c/0^)5.b55)/8,^/085deb+5/)0,5f8g*+;8**c8b/\03)p+^
/0g8d+3+5+83.\^+.)5)0,50,a+\8*>0>/\+8^b*/`83/).)`8,/b,8a*+/0.0,5+,/8,^\8]+/\+8b/\03)/c/0`30])^+
.0,5+,//0/\)55/b^cfV58``*).8a*+q/\+),>03g8/)0,),/\+8a0]+.0,5+,/r85^+5.3)a+^/0/\+8^b*/`83/).)`8,/
b,8a*+/0.0,5+,/r\08;3++5/0`83/).)`8/+),/\+5/b^cf
-);,8/b3+0>XVW
23),/8g+0>XVW
78/+
jh[
-);,8/b3+0>,]+5/);8/03
23),/8g+0>,]+5/);8/03
78/+
stmu[
vwxz{|x}w~
vwxw}~


	

		
 !""$%&"'
()*+),-+./)0,%1230/0.0*40,5+,/$&'
6+35)0,78/+19 :9!: 9 &
28;+<=0><=
??ABCB?DE?FGCHAIJGCKLMNLOMLNO
P	Q	

4R-STTR2UVT42UTSU4W4UWVS-SUV4-TX7Y
-);,8/Z3+0>[)/,+55\
23),/8]+0>[)/,+55
78/+
\^ P_	

`	
PQaa^b

c
U,),/+3d3+/+3e030/f+3),g)h)gZ8*eif05d+8j5S,;*)5f8,g/f+d83/).)d8,/k5d3+>+33+g*8,;Z8;+>8.)*)/8/+g
/f+8g]),)5/38/)0,0>),>03]+g.0,5+,/8,g5+3h+g858i)/,+55lTf+),h+5/);8/030m/8),),;.0,5+,/]8n,0/
8*505+3h+85/f+i)/,+55l
U,),/+3d3+/+3e030/f+3),g)h)gZ8*eif05d+8j5S,;*)5f8,g/f+d83/).)d8,/k5d3+>+33+g*8,;Z8;+>8.)*)/8/+g
/f+8g]),)5/38/)0,0>),>03]+g.0,5+,/mZ/g)g,0/5+3h+858i)/,+55lTf+,8]+037.0g+0>/f+d+350,
d30h)g),;),/+3d3+/)h+5Zdd03/)51
l
opqstuqvpwxyzx{|x
opq}~~p}}vwx{yx{