> There is a long-standing war by cryobiologists on anything to do with cryonics - the old canards like the exploding lysosomes o' death may have been refuted but last I heard, they were still explicitly banning from membership any cryonicists

I'm not sure what to say here - politics definitely plays into biology publication but there are multiple well-respected journals to send 'small results', of which I don't believe Rejuvenation Research is one.

> Look up the journal policies and note the dates

I did exactly this. Unless Rejuvenation Research has an unusual application of the terms 'Received' and 'Accepted' (see ref. 1), I'm more inclined to believe the information on the article page than a general journal policy.

Weirdly enough, all the articles in the most recently published edition of Rejuvenation Research (http://online.liebertpub.com/toc/rej/18/2) had at least some gap between receipt and acceptance. I'm even more inclined to think something unusual has started to happen with the current batch of online-before-publication papers. This could, of course, be a bug with the journal's content management system.

> Reference 14 is to the SafeSpeed cryopreservation protocol

Publishing a reference to an non-peer-reviewed protocol is unusual, irrespective of the details. This is even more unusual when the protocol forms the core of the paper.

To be a bit more precise (using details from the paper):

Vitrification and slow-freezing are distinct methodologies for preserving specimen. The authors only use two methodologies for preservation, which they explain in the methods section:

The cryopreservation methods included worms that were trained and cryopreserved by vitrification and worms that were trained and cryopreserved by slow freezing

> If you distrust SafeSpeed, you can throw out that entire part of the paper and the results are exactly the same: preservation of olfactory memory

I think this is part of why my original comment said we should wait for replication and be skeptical. Trust in science is a difficult thing to gain and an easy thing to lose. The choice of journal and the very-unclear description of the cryopreservation protocols make me a bit more skeptical of the results.

The SafeSpeed protocol seems to be under review at Nature Methods. If it's accepted there, the authors will likely need to supply supporting data which will only bolster the claims they made here.

Further, the slow-freezing result is a fairly cheap one to replicate; we're likely to see many people try it and they'll likely cite Alcor's work.

> The 30 minutes refers to the traditional vitrification itself

I think the authors were intentionally vague in comparing the two preservation methods, especially given how clear the rest of the report is.

First, note that only one vitrification technique was used, SafeSpeed. Additionally, only one slow-freezing technique was used, a standard approach that one of your sibling commenters references in passing.

The SafeSpeed-vitrified animals were stored in liquid nitrogen (LN2) for 30 minutes and then recovered. This is stated (unclearly) in the methods section (pg 8) and alluded to in the discussion.

> 30 minutes refers to the traditional vitrification itself (this is why it's 'slow')

This is counter to what the authors state - they explain that the SafeSpeed method is actually slower than 'slow-freezing'. See page 11.

Conclusion

I think that this will be exciting if SafeSpeed gets published somewhere more reputable. As you alluded to earlier, if there is a physical correlate to benzaldehyde training that isn't damaged upon freezing, we'd definitely expect it to be recovered after freezing.

This paper just isn't compelling enough to say that we've seen this occur definitively.

References

1. http://online.liebertpub.com/doi/10.1089/rej.2014.1636

'Received' usually indicates receipt of a manuscript;
'Accepted' usually indicates the end of scientific edits.
Often, articles are held up (i.e., not published online) to coordinate
publishing with other labs/projects within a lab.