skysight-inc/HackerNews huggingface dataset:
The mice used in the protocol were SCID mice [1]. They do not produce B or T lymphocytes (and thus no Ig either). Pretty much no adaptive immune system to speak of. These are a mainstay of murine study.

The discussion section referred to the existing technique of islet transplantation [2]. This is sourced from cadaver tissue, and as such requires immunosuppressants. Obviously needed to counteract host v. graft, but I wouldn't be surprised if it masked the autoimmune recognition.

The discussion section alludes to several future hurdles: gene expression differences from primary cell sources, transduction response, local tissue effects (need for tissue engineering), need to produce alpha, gamma, delta, and epsilon cells (alpha and gamma may be important to precise beta function), questions regarding long-term maintenance of phenotype, ...

Nevertheless, this is very exciting! Stem cell researchers are truly hackers in every sense. The results here aren't only applicable to diabetes research, either. Patients aren't the only ones that need these cells.

[1] http://en.wikipedia.org/wiki/Severe_combined_immunodeficienc...

[2] http://en.wikipedia.org/wiki/Islet_cell_transplantation
Fun fact: the (Australian) company supporting Prince has the (Norwegian) Håkon Wium Lie as director -- CTO of Opera and the proposer of CSS (see recent article https://news.ycombinator.com/item?id=8436659 ).

Håkon / Haakon being a common name, coincidentally the Norwegian crown prince is also named that. So searching for "haakon prince" has two quite different meanings.
The mice were SCID mice. They have no adaptive immune system to speak of. I made another post in this thread and included some links.

This is a very incredible result though. Even if immunosuppressants are required, think of all the non-patient outcomes: easily sourced human SC-beta cells for research and high throughput study. This stuff can be grown at volume, and the techniques will only improve. The results will also inform research into other cell lines. Bottom line, this is yet another step forward for stem cell tech.
I study.
Like many people here have mentioned, mastering everything can take far too long, and is completely unnecessary in practice.

It is much, much more important to master the foundations, which consist of: 1) Probability and statistics 2) Linear algebra, and 3) Programming proficiency

To be more precise here is what you would need to know in each area:

1) Probability and stats:
Properties of the most common distributions (Normal, Poisson, Exponential), conditional probability, properties of expectation, Bayes rule and its applications, hypothesis testing, confidence intervals, bootstrap / jackknife, know how to compute the power of a test, be able to run Monte Carlo simulations to model real world phenomena.

2. Linear Algebra:
Know enough to understand and compute Eigenvalues / Eigenvectors, SVD and various matrix factorizations.

3. Programming proficiency:
Algorithms and design patterns. Its enough to know the typical undergraduate algorithms course content: trees, heaps, hash tables, various sorting algorithms, graph algorithms: shortest paths, spanning trees, max flows.

If you know this, you can easily pickup everything else on a need-to-know basis. Depending on the domain you are working in, that might entail either natural language processing, computer vision, machine learning or big-data tools like Hadoop / Spark etc. All of that is very easy to learn once you have the foundations.
Stuff like that is the reason why I'm excited for Rust's future. It's really an amazing language.
They probally have enough information to convict him. But you know that Kilos is just how most of the world handles weight? If I wanted to put lets say a speaker or computer in there, id also be interested in what weight it can hold.
If they actually wanted to carry money, they would also need to know as money is pretty heavy.
Sorry, for the saving-40% figure I was talking about me, not the hypothetical person who makes $130k. I should have been clearer on that point. And I was also talking about 40% post-tax, not pre-.

Still, I'm not understanding how you think a $3k/mo rent isn't possible on $130k/yr. I personally know people who do that.

Let's be conservative and say 40% tax rate. That's $78k left. $3k/mo on rent is $36k/yr, which drops you down to $42k. If you can't eat, drink, and be merry on $42k/yr, and still save some money, I would seriously suggest you take a hard look at your priorities.

I wouldn't say that at that level you'd necessarily want to sock away 40% (again, post-tax), but that's even doable if you're living on your own and don't have dependents: 40% of your post-tax pay on $130k is $46,800. Subtract $3k/mo for rent, and you have just shy of $11k free. I'm not saying you're going to have tons of fun spending only $11k/yr on food and entertainment, but it's certainly possible. But instead dial it down to 30% or 25% or whatever. I bet at that level you'd still be saving more than the average American.
I thought venue was a huge part of US law.
Wasn't that how Weev's conviction got overturned?
How I prepared for GRE
Bitcoin’s Killer Apps – A Look into the Future
> I've always felf the entire brew pod setup is an answer to a single question: "how do you get customers to accept cafeteria margins on their homebrew coffee?"

There's also "how do we stop losing business to companies that don't abuse the shit out of their suppliers?" There has been an increasing amount of noise over the last 5-10 years about the phenominal margins that exist in the coffee business, with growers typically seeing the barest fraction of what companies like Nestle sell coffee for. As companies selling fair trade coffee become more profitable, what are companies like Nestle to do?
I absolutely did not ignore income tax.

I did ignore children, fairly deliberately. If you do have kids, then hopefully there are two parents, both with income (yes, I know this isn't always the case).

Four people (two parents and two kids) will live much cheaper together than four people living on their own. Kids are expensive. I get that. That changes the equation quite a bit. I'm not particularly equipped to do that math since I don't have kids and don't know what they tend to "cost".
http://www.amazon.com/dp/B00I15SB16
http://synergy-project.org/
You're talking of Shamir's Secret Sharing Scheme (alliterative translations in other languages left as an exercise to the reader)

It's really neat, actually and can be efficiently and easily implemented just going from wikipedia's description.
Theree are hundreds today. The objective around systems like this and Nespresso is to move coffee making to the same kind of vertical integration inkjet printing enjoys. Loss-leading on selling the devices is a great first step if you're the prospective monopolist.
If SyncTeX really works out-of-the-box, that would make Emacs an even more powerful LaTeX IDE than it already is!

Right now I use it with Evince and SynTeX works forward (I can jump to the position in the PDF in Evince where my cursor is in Emacs) but not backward (I can't jump in Emacs to the LaTeX source corresponding to where I am in the PDF in Evince). I never managed to get it to work.
I think it's a reasonable comparison, yes, but not solely on monetary terms. Cost of living is certainly an important part of a decision on where to live, but it is not even close to the only consideration.

Put differently: the size of house you can buy is often low on the list of considerations.
A whole server tower filled with kvm racks? http://static3.bigstockphoto.com/thumbs/6/1/1/large2/1160690...
The paper was fantastic! The discussion section is a must-read for seeing where this is going.

The calcium ion flux response to glucose challenge was neat. Demonstrates the signaling mechanics being as expected.
Lecture 5 – How to Start a Startup
Realistically... the biggest reason for irrelevance is Microsoft's concept that an operating system should be a prime source of revenue. An OS is the garden you want people to come to so you have prime real estate to sell your wares. Charging admission works great if you're the only garden in town... and not so much if you're not. Microsoft is putting itself into this position by forcing the market to move into the other, more easily accessible gardens. It has very little to do with UX/UI or anything like that. Most consumers actually don't want to learn how to do anything new if they can ever help it.

I'm constantly amazed that all these "experts" haven't figured out what happened to the 800 pound gorilla. Quite simply put, other gardens that people could live with and easier to access suddenly showed up. The vendors making and selling their wares went to the place people were at or wanted to go to. Its simple economics and you can point directly to the people who decided that WGA was a good idea for killing MS. Piracy itself is what made windows dominant to begin with.
I have none. His website looks nice, his business model is sound, and people are talking crap about it simply because they don't personally see a need for it. I felt like defending that.
In the mid 80s I had shortly this impression that computer science and programming now are mostly done. It was when I had read the books (blue, green, orange and purple http://www.world.st/learn/books ) on Smalltalk 80. OOP, large class library, UI, ... All done. What would be needed more?
Then -- if people polled so believe -- they should get off their lazy goddamn asses and vote. We did have such a vote on marijuana recently in California, and we lost. We'll probably have another vote in 2016. But if all people are willing to do is run their mouths to pollsters, then we're going to have another 40 years of the war on drugs.

Showing that allowing people to smoke a bowl at will doesn't cause society to crumble will help when we discuss harm abatement projects for heroin, meth, etc.
An Objective-C 3.0 What-if
So, I hear that touted as "conventional wisdom", but it's not particularly clear why. As long as you aren't spending beyond your means, what's wrong with allocating more money to housing? Say I want a bigger apartment, and I'm willing to drastically cut back on restaurant outings to make up the difference. How is allocating 50% to housing and 25% to food (assuming that 25% buys you healthy meals all year) less sustainable than allocating 25% to housing and 50% to food (talkin' 'bout some pretty nice food here)?
I think you should discontinue this piece of crap. It even probably (depends on whether you perform some sort of sanitization before passing request) has SQL injection vulnerability in your "todo" app example. And it looks like piece of crap that would noone ever use.
Why cant I submit this story on HN?
Don't be ignorant. I am in Canada and this guy is in California. I have never met him, have no link to him in any way, and just found this site like 3 days ago.
PS: Quite ironic to see him saying "get rid of Google", through an Hangouts session.

It's only ironic if you didn't read the first half of the title. He wasn't looking to keep this call private, was he?
Dude is american. Outside of science, there are only a handful of things in America measured in kilos or grams. I'm struggling to think of one besides drugs. Certainly coke or heroin. Pot is often sold in eighths, while hash is sold in grams.

Nobody in America discusses weight of speakers, computers, or anything in kilos. Perhaps the only other place I've heard it used is for bumper plates. I'd frankly bet more than 90% of Americans couldn't tell you off the top of their heads if a kilo weighs more or less than a pound.
HTTP/2: The Long-Awaited Sequel
> I said that making decisions at the individual level about which laws are rational and breaking the irrational ones is not feasible.

What do you mean by "feasible"? I am certainly capable of deciding which laws I think are rational and choosing which laws to obey.

> Civil disobedience is almost never undertaken at the individual level, and more specifically it is never successful at the individual level. It is an exercise undertaken by a society.

You seem to be conflating civil disobedience with organized protest or resistance. Civil disobedience is just a deliberate refusal to obey a rule one believes to be a bad rule. It doesn't have to be organized.

You seem to have a disdain for all individual discretion about rules and laws. I disagree, and strongly. Only individuals act, and I think only individuals have the ability and responsibility to choose how to act.
Yes, but it seems like it has effects simular to a slight depression on men. So it impacts quality of life, at least thats the impression I got. So even if it does not cause huge costs for the health care system or unability to function in life or work, it still seems strange to just ignore it. At least pregnant women are tested it seems.
This site doesn't have a way to report troll posts like yours? Shame. Doubt I will make it a habit to visit here.
> But you know that Kilos is just how most of the world handles weight?

Yes, but not Americans. 9/10 times, if an American is talking about kilos they're talking about drugs.
How on earth is MFC obsolete? Old yes, but it works fine. If you want a standard Windows application in C++ it's what you use. C# is more common for new projects though, and replaces Visual Basic as the line-of-business app builder of choice.
Planning such an awful system gives it false legitimacy.

"Look, guys, I'm not saying New Earth Creationism is right; I'm just saying we should have all our scientists talk about it and find arguments in favor of New Earth Creationists."
That argument just boils down to "we overreact to terrorism, therefore terrorism is worth overreacting to", though.
Sorry, but this is just wrong. Any system that offers multi user sync is inherently more complex than it needs to be as a backup solution. A backup should generally be

- convenient enough that you do it without thinking about it.

- technically as simple as possible, so it's easy to understand and review.

- secure.

Dropbox fullfills the first point, but not the second, and the third is debatable. Spideroak as a counterexample is just as convenient, has a pure incremental backup mode and is client-side encrypted, the gold standard of security.
Because in a technical crowd not using Chrome is a conscious choice rather than an oversight due to ignorance.
We use BIRT to design & run our reports. It is flexible and renders in all sorts of ways (including PDF). We have not had major issues with it.
SSRS is a beautiful piece of software.

SQL Server Express with Advanced Services ships with SSRS, is free, comes with all the tooling and generates PDFs too.

Perhaps surprisingly, if you install your ODBC drivers for your database engine (MySQL, Postgres etc) you can create a report from that data source as well so you're not tied to SQL Server as a database engine.

Typical steps: http://www.mssqltips.com/sqlservertip/2615/creating-a-ssrs-r...

And report builder: http://www.youtube.com/watch?v=Iy-bE0yXGlk

Can't beat it for $0.
Do people from Canada, Mexico, Peru, Brazil, etc identify themselves as "Americans", though? My meager experience has been that most people identify with the name of their country, not continent.
The list of topics at the end isn't necessarily "very easy to learn", but I do agree that fundamentals are essential.

Depending on the mathematical background of the individual, they may need calculus and general mathematical maturity / ability to solve non-trivial math problems before venturing into the data science field.

Also databases. Absolutely need this in the fundamentals, arguably more so than graph algos etc.

Edit: English
3-4 seconds is extremely fast if you're coming from the C/C++ world. I can't see how this could possibly be a roadblock.
I wonder how many Hiro Protagonists will be running around after this really begins to catch on.
Thank you for putting this together, looks like a great collection of resources.
The problem with voting is that the individual benefit of voting is almost certainly less than the individual cost of voting. To expect most people to educate themselves about political issues and vote is to expect most people to act irrationally.

If, on the other hand, individuals were able to choose to decrease their own taxes by whatever portion is used to the fund the drug war, I suspect people would be more educated about how the drug war works, and the drug war would have a lot less funding.

Conversely, if everyone voted on what car to manufacture, and only the winning car could be legally manufactured, I suspect people would do very little research of cars, and the winning car would be an awful car.
A wiki about useless information
A similar project, Quake 2 map renderer written with Julia https://github.com/jayschwa/Quake2.jl
I've started playing flag football after playing football 10 years ago. I've stopped playing football because I tore my ACL in both of my knees.

Beeing a defensive end / outside linebacker I can say that the bigger positions don't really translate to flag football. You can't really block other people without real contact. We are playing 5 on 5 - but I can't imagine how contact less football would work 11 on 11.

I enjoy playing some flag football, because it's a nice workout and some of the aspects of football are in (different coverages, beating routes...)
If everyone was constantly breaking the law then there would be no known criminals who haven't been arrested. There would no John Gottis or Whitey Bulgers. As soon as the government wanted someone arrested, they would just immediately arrest them for breaking copyright law or whatever.

The unquestioned premise in your argument is that the government wants all criminals to be arrested.
In that veins, I'd love to see a list that focused more (or even exclusively) on the fundamentals. Do you have any such recommendation?
There are some players who are not necessarily the best athletes on the team, but can excel with better instincts or a better way to analyze the game.

But in the NFL the level of athleticism is so high - obviously there aren't any bad athletes in there.

If you know exactly where to run beats running (a little) faster.
pandoc is great too. you supply it with e.g. markdown and it can generate many different output formats. pdf via tex is one of it.
>But I'm bewildered by this article. It seems really damaging, and like it doesn't really add very much to the corpus they've already published.

Really damaging for whom? 99% of the worlds population are victims (them or their countries) to the stuff described in the article, not cheering for its continuation.
Story of We the Chic
>On the other hand it deals a pretty big blow geopolitically/internationally.

So? For me (Godwin's law be damned) it's like leaked documents about Nazi germany practices. If you were not a German you'd cheer, and if you were a non-Nazi German you'd also cheer.
Please don't do sockpuppet posting on HN.
That would require reverse engineering the application running on the cloud server and the transfer protocol so that you can host an equivalent version yourself. It can be done, see for example pirated Battle.net servers for the Warcraft games, but in many cases the effort simply is too much.
Curmedgeon here, but if you want a small, easy to use, cheap coffee brewing system, what's wrong with a french press? I got on for €6 and it works great. Far less plastic waste, too.
Not really: we have hi dpi (and in focus) resolution only in the narrow field of view in the middle, everything else is not that good. We compensate for this by having ability to quickly move eyes and refocus.
and cheaper even

You can get ridiculously good coffee, brewed strong, for less than a k-cup costs
Anxiety occurs for multiple reasons, so false positives would always be a confounding factor, even if micro expressions occured 100% of the time.

There is quite a bit of evidence that even with physiological measures linked to stress and lie detector machines, there is little reason to believe lie detectors are especially effective. Given that non invasive means of detecting anxiety are limited, and anxiety is itself limited, such screening is going to not be helpful.

That said, there are some great studies on using recognition and EEGs or fmri machines to detect lies. Your brain processes item's you recognize in different ways, so if someone maintains they never were in an apartment where a murder occured nor had seen it ever, you could display a series of apartments, including the murder scene, and be able to tell when they recognized the apartment. It's known in the field as the Guilty Knowledge Test, or GKT. Check out the work by Farwell and others if you are interested.
Why do you assume I eat undercooked meat or unwashed vegetables? I was talking about myself and my family, not what everybody else should be doing. I'm Bulgarian and the meat we eat is either well-done (roasted or fried) or boiled (healthiest, no doubt) - we don't ever eat rare meat. That's why we don't like beef - it's hard to chew unless it's rare. Regarding the veggies, our culture is to eat veggies in soups or fried (not ideal, I know), and we eat limited veggies raw, but most of them area easy to clean (like tomatoes, sweet peppers, and cukes), but, in general, washing well or cutting outer layers (which usually contain the most nutrients, I know) is done as people are very cautious. I'm always surprised how farmers markets here in the States give samples from unwashed produce - I'm literary getting grossed out! Some of the germs on them are beneficial, I know, but the risks outweigh the benefits.
but how will someone charge you 50c a cup for stale shitty coffee?
With tea we do not have such problems :-)
Well, it's already in consumer-level (ok, "prosumer") cameras. Also we know that "fps" is of an eye is around 60 Hz - since it's the minimum that monitors looks OK.
There is no exaflop computer in the human though - we just don't have enough energy to power it inside us.
How to Increase Revenues by 304% Using Emotional Targeting
One of the most important and unique things about emotional conversion optimization is the possibility of increasing revenue right from the first step of your funnel before starting to optimize the checkout process. In less than 3 weeks the tested strategies increased signups by 38% and more importantly increased the number of paying users by 304%.
>I don't think it is an oversimplification. Society as a majority, at some point, wanted these drugs illegal.

Not really. There was an orchestrated campaign, with state support after some point, to get to this.
Believe me, you would get a queue around the block of people with IBS who would be willing to take this pill if there is scientific evidence that it works.
Asset seizures fuel police spending
ctrl+break does the same on windows ping.
Chinese translation:

http://book.douban.com/subject/3202119/discussion/1364384/
On Page ASO Tips
I always try to tell myself this. At the end of the day, I still think it partly still has to do with "luck". Who knew bitcoins could explode? Just strive now to find the next big thing is all we can do...
That's not what he's asking for. He wants multiple books open at the same time with easy access and in view, I suppose. Of course, you could have more kindles, like a bunch of the star trek [PADDs](http://en.memory-alpha.org/wiki/PADD) lying around on your desk, but the kindle is too expensive for that at the moment. And even then, when I am doing historical research or writing a conceptual piece on some scientific or technical topic, it is not uncommon to have ten to twenty books, articles, documents or artifacts open at the same time and it never fits on my desk comfortably. So I end up using multiple table-like areas on different heights alongside my desktop: a coffee table to the side of my chair, another chair in the corner, an open closet with shelves just below eye level, and so on.

A document "wheel" or carousel would help a lot.
Unix-style note taking via email