Sperm preparation with Spermfuge
A laboratory centrifuge is a piece of laboratory equipment, driven by a motor, which spins liquid samples at high speed. Laboratory centrifuges work by the sedimentation principle, where the centripetal acceleration is used to separate substances of greater and lesser density.

Spermfuge is an improvised centrifuge, dedicated totally for elevating the total motile sperm recovery from the parent semen sample, by controlling/maintaining the main parameter of "Temperature" thus aimed at Enhancing the ART results.The goal of all sperm preparation techniques is to improve sperm quality in ART. The ejaculated spermatozoa have to be separated quickly and efficiently from the seminal plasma so that they can be functional. The two routinely used preparation techniques are swim-up and discontinuous density gradient techniques. Since men with abnormal semen parameters do not yield adequate motile sperm, it needs to be processed prior to its use in ART. The instrument has been designed to regulate and subsequently maintain the "Critical" inner chamber temperature before, after and during centrifugation. Maintenance of this critical temperature throughout the entire sperm processing procedure helps the sperm maintain their peak motility and enhanced linearity. The two of the most vital parameters which act as a yard stick to gauge the effectiveness of an ideal sperm wash. The existing dependency on RPM will be eliminated by on screen g-force selection.

The main objective of the spermfuge is to evaluate the effect of temperature on the sperm motion characteristics and subsequent outcome of sperm preparation.

Use of temperature regulated centrifuge (Spermfuge) improves sperm quality.Thus spermfuge acts as the two of the most vital parameters which act as a yard stick to gauge the effectiveness of an ideal sperm wash in all Ivf IUI ART ICSI labs and clinics. Sperm retrieval techniques form an integral part of the assisted reproductive programme. The success of sperm separation is measured by the number of motile sperm retrieved from a given semen sample. The study aimed to evaluate the effect of temperature during sperm preparation events on the number and percentage motile sperm retrieved following a double wash swim-up procedure.

The design of the spermfuge has been framed after taking consideration detailed cellular physiological demands, locomotion kinetics and pooling together ideal suggestions, opinions, ideas and propositions of numerous Andrologists and Embryologists and other professionals in the field of Human Reproduction. The Spermfuge with its Temperature controlled chamber (room temperature to 42°C), programmable microprocessor based controls, multiple tube selection, accurate g-force selection, independent centrifugation and/or heating selection and other added advantages make it an ideal state of the art instrument for use in an ART laboratory.

The heating range of Spermfuge's chamber is from 2°C above ambient to 42°C.Unless and untill the set temperature is attained the intelligent control system does not allow the centrifugation to commence. However the ART Laboratory have the option to commence the centrifugation without chamber heating and to commence the chamber heating without centrifugation.

Various clinics, laboratories and regulatory bodies have their own different set of protocols to be followed. The Spermfuge facilitates user selectable 9 programs for different applications. The user can select RCF, RPM, Tube Size, Temperature, Time for each program by a digital encoder as per the requirement of there clinic. Thus spermfuge has the capacity of multiple programs.

An adjustable brake is provided to prevent the rotor from stopping too quickly at the end of run which might cause disturb to the firmly formed pellet. One can select 3 mode in acceleration and braking, slow medium and to fast can be given on screen.

The exact indication of G - Force helps the ideal sperm pellet formation by subjecting the sperm to an exact and precise amount of G - Force unlike other Centrifuges where G - Force is subject to RPM and the tube size. Now a days centrifugation speeds are never reported in RPM as those numbers are not useful for any one wanting to reproduce the method. (Being dependent Rotor radius.)

Spermfuge has a swing out rotor head to accommodate 8 tubes maximum of 15 ml. The Sperm Fuge can accommodate 6ml Tubes (Falcon 2003), 14 ml Tubes (Falcon 2001) and 15 ml Tubes (Falcon 2095). The tube selection can be done on screen with the help of the encoder, which ensures an exact G - Force selection.

The Vision port in the lead at the center helps to confirm rotational speed with a stroboscopic device. The safety interlock in the lead prevents accidental opening once the Rotor is operational. The maximum RCF is 800g which prevents accidental damage to the sperm.

State of the art micro-controller with optomagnetic encoder provides intelligent control system. Digital programming via microprocessor control for various parameters including temperature, RCF and time. The parameters are displayed on four line LCD display.

The Spermfuge is virtually maintenance free since it utilizes brushless drive motor. The motor is extremely smooth even during running at the maximum speed. The motor is electrically controlled during the acceleration and breaking with smoothness.

Centrifuge temperature and incubation temperature significantly influenced the percentage retrieved motile sperm. The use of temperature-controlled sperm preparation might have clinical value for men with poor sperm motility values.

An efficient sperm preparation method is one that preserves sperm motility, viability and fertilization capacity during assisted reproduction procedures. Temperature regulated centrifugation (Spermfuge) improves sperm motility and longevity
when compared to a non-temperature regulated centrifuge.

Shivani Scientific Industries is exporter, manufacture and supplier of ivf lab equipments like microinjection tools,ivf workstations,Spermfuge,ThermoPlate, Embryo Freezer,System for ART,Suction Pumps for oocyte aspiration,CO2 incubator,IVF warming block,IVF micromanipulator,IVF Microscope,IVF Micropipettes,Clean Air System, Spermfuge,Mobile Nest,Lab Guard,Warming Blocks,Turnkey Projects,Rapid Recovery System and lab designs to IVF clinics worldwide. We offer products for IVF ART ICSI including micropipettes,microscopes etc.

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