skysight-inc/HackerNews huggingface dataset:
What will Cause Cell Line Contamination?
Cell Line nature enemies include: Bacteria, viruses, mycoplasma, yeast and other eukaryotic cells. in the culture procedure, what we offered below will lead to cell line contamination:
A. Confusion of the Mark, which refers to paste the wrong label in the the daily culture and the management of the cells.
B. Cross Contamination of Cells, cell line is contaminated by other cells due to cell reagent utilities, reusable or simultaneous operation of a variety of cells and other reasons. If exogenous cells have a faster propagation rate than the original cell line, exogenous cells will gradually replace the original cell line.
C. Cell culture enviroment change will lead to cell line contamination. Besides, bacteria, mycoplasma contamination or drug treatment may also change the type of cells.
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Why it is important for avoind cell line contaminations
Cell line contamination poses a serious threat to the integrity of biomedical research. Following best practices for cell culture validation, storage and processing can help address this ongoing challenge, thereby improving the reliability of experimental research data.
I'd say going with more than one language will just confuse beginner. I've seen many students struggle with the language soup schools cook for them.
For one, the use of the word blockchain itself is posing a problem. If I remember correctly there was a case made to use the word - distributed ledger instead to avoid confusion.

And I have never understood one part of it. How are contentious settlements are resolved? Today for any disputes people can approach BIS or DTC and once issue is resolved everything can be rolled back. Who acts as the "watcher" in this case? Surely, going by what majority says is not a solution.
How to Avoid Cell Line Contamination - Mycoplasma detection
1. Mycoplasma culture, which is regarded as a standard method of detection. This is the most sensitive method, depending on the standard agar plate on the selective and highly enriched growth medium. The appereance will be unique ‘Omelette’, the positive results with this method can prove the mycoplasma contamination. However, this method can not detect all the speices, such as Mycoplasma hyorhinis, DNA and PCR analysis is needed to ensure there is no contamination happened!
2. DNA Staining: DNA staining relies on the Hoechst 33258 stain, which causes DNA-rich nuclei and any mycoplasma in the cytoplasm to fluoresce. The use of this method may lead to false positive for the contamination due to cell apoptosis or debris, or false negatives as this is the least sensitive method.
3. PCR: PCR-based mycoplasma detection can be very sensitive and can detect up to 20 copy copies of mycoplasma genomes in 2 μL of sample. Mycoplasma detection was achieved by amplifying the highly conserved 16S rRNA operon coding region of the mycoplasma genome.
4. Cell-Line Authentication. ICLAC, which co-built by ECACC, representatives from other internation cell culture organisations and respectable scientist commit to support the importance of cell line authentication, creating a searchable database of cross-contaminated and misidentified cell lines. This can be used to detect cell line contaminations with the regularly updated database.
5. Short Tandem Repeat Profiling. With ASN-0002-2011 standard method, which analyzed 13 specific sites of STR and several additional sites to determine the integrity of human cell lines authenticity.
Calculations details are here:
https://github.com/tmrowco/electricitymap#carbon-intensity-c...
We use IPCC LCA data for the production mix, and use a coupled linear system of equations to take into account imports in a dynamic manner.

Generation data is realtime and comes from ENTSOE (https://transparency.entsoe.eu)
Yes very interesting topic for me too. Especially this kind of filter(UKF) present relatively new evolution of Kalman filters designed to outperform EKF in some of the cases. The idea of my project is to colect examples for different engineering applications and in the same time to test my library.
There's also http://simplepie.org/ for those who don't wish to roll their own.
I don't think it's particularly helpful. There's not much to be done, apart from write documentation explaining why what he thinks is bad is actually good, I suppose.
France has always been investing in nuclear energy, https://en.m.wikipedia.org/wiki/Électricité_de_France
Thank the "environmentalists"
Fourquestions every marketplace startup should be able to answer
As far as I can tell, the purpose of tokens is to provide an incentive for running the ledger client and processing transactions: mining for uptake and transaction fees to maintain scale long-term. You need as many people to run the client as possible.

If you can find some other reason for processing transactions and can guarantee that the population is big enough then the coin becomes unnecessary.
I seem to recall that the first action of the British in WW I was to cut the German communications cable in the Atlantic.
> That's a ponzi scheme.

In no away shape or form a ponzi scheme.
I remember it being quite a revelation as to how discrete digital circuits can be optimised Our Uni class execise revolved around designing a drink vending machine operation with a minimum number of logic gates.
France has a carbon intensity if 61. Nuclear power really helps. NY state is also under 200 and uses quite a bit of nuclear power.

It’s unfortunate that nuclear energy is not acceptable to many people. Hopefully, China will be successful with its adoption.
It sounds like a motivation problem. Are they getting enough equity? Are the rewards big enough? Do they trust that it can be successful with the time they need to put into it?

Something that is large and intimidating, but potentially life changing, is exactly what would make people procrastinate forever.

I would say you should aim lower. Make a subset of the big idea. Hack it as fast as possible just to see if it would work rather than trying to build it right. Assume the MVP is there just to gather information and not the actual product.

Once you get to the point of "I can finish this next week, no problem", it becomes a lot more motivating to do.
Text decoration none? Seems like a really bad idea, can't tell what things are actual links.
Microsoft Windows enforced maximizing everything like that since the 3.x days. Every other OS tried to enforce not doing that - some taking it to an extreme! I recall when the "maximize" button in OS X was a "zoom" button that stretched the window vertically, but it'd only stretch a window as wide as the content demanded.

Of course, the Windows users whined and whined and whined about that until Apple decided to make everything go fullscreen super easily.

So anyway, I love seeing people use 34" ultrawides to browser websites with their browser window maximized. It's a ridiculous waste of space. XD
Being Italian I can attest that I spent most of our high-school studying and celebrating such style of writing, and the rest of my life being criticized for it. Dammit ;)
http://mix.stromhaltig.de/gsi/
Eleven Linux syscalls that suck
Let's put wind farm on climate change amplified tornados and profit.
Impraise (YC S14) Is Hiring a Product Manager in Amsterdam Passionate about B2B2C
France's 2017 nuclear generation is well below record levels:

https://www.platts.com/latest-news/electric-power/london/fra...

According to S&P Global Platts calculations based on reactor availability, output is unlikely to reach 380 TWh this year.

Based on cumulative output of 361 TWh by December 17, output would need to average 56.5 GW over the final two weeks in 2017 to even reach 380 TWh, the TSO data-based calculations shows.

Nuclear output averaged just below 50 GW so far this December, up from 42 GW in November, the data shows.

...

EDF operates France's 58 nuclear reactors. But following record production years in 2014 and 2015 at 416 TWh, it has struggled with extended outages and lower nuclear production.
> the department’s $543 million contract with Hewlett-Packard

> The system [...] has been hailed as a way to potentially save millions of dollars

Does not compute.
What stops a 90%-pure language to dectect pure functions and do the exact same optimizations?

I'm not saying that is decidable in all cases (can't come up with a meaningful counter-example either), but usually a 90% good enough solution is better than a 100% solution that makes you go out of your way to do very simple things (like print to stdout).
Worked as a trainer to teach new employees how to load trucks in college at a large UPS distribution site. There is an ideal way to load trucks for safety and efficiency i.e. heavy items on the bottom, small light items on top, and hazmat on the bottom sides. This is easy to achieve when the package traffic is light and possible even during moderate package flow. You can think of this process as physical Tetris, which is a great workout. Unfortunately when the package load is very high, loaders just end up hapzardly loading trucks to keep the package flow going and to meet nightly deadlines of filling x number of trucks. This problem was compounded by a high turnover rate, which didn't exist before the addition of health insurance effectively cut hourly salary in half. This process may be more efficient today with better scanners, that hopefully no longer requires loaders to manually key in tracking ids.
TLDR:

* use like a dozen Adblockers likely all using the same filter lists slowing the browser down

* use some Antivirus from Russia to grant access to your whole system

* use a third party vpn client for the one built into iOS

* use secure passwords
Sarcasm can be viewed as hostile. It's a good way to make enemies for no reason.
Yeah, I have a similar recollection of lectures at college 30 years ago, and what seemed to be a hugely complex system being reduced drastically in complexity by the realisations contained within the maps. It was obviously a contrived situation that the lecturer had thought of, but there were many 'lightbulb' moments across the room in that hour.
That's amazing! Nicely done! Kudos to having enough drive to finish a costume 2 years down the road!
Nuclear is not very renewable since its fuel supply is limited (although very large compared to fossil fuels).

Dumping waste in the ocean is not a great idea, because you can't be sure that fishing grounds won't be contaminated. Nobody wants radioactive fish.

In general, I think the best solution to long-term storage of nuclear waste is to keep it at some facility where it can be continuously monitored. That way, you at least know when something leaks. Unfortunately, energy producers are really good at not paying for that kind of externality.
While I appreciate the effort, the map is misleading as the scoring/coloring is purely focused on Carbon emissions.

This means things like Nuclear power, ecologically disastrous in the long term, get a nice thumbs up (see France e.g.) because of their short term 'benefits'.
At least in Japan, many toilets also have dryer system built-in along side the bidet. Others have toilet paper for drying off.
I’ve dont something simple but similar to this with UV unwrapping in Blender directly. You can even export the UV map to SVG.
Neat. I wonder if this could be adapted for sewing patterns. The open-source options I've seen out there seem to be lacking (abandoned, or compatibility issues), and the commercial options are very expensive.
Very cool.

I'm using UK-specific carbon intensity values, and breaking down different uses of the same fuel (OCGT & CCGT). I've not baked in transmission loss yet. Our results are surprisingly similar though.
Consumer electricity price in Germany is almost twice the French price.
Edit: source https://en.m.wikipedia.org/wiki/Electricity_pricing#/media/F...
those are 2 separate issues (# of hrs you can dedicate per week, vs, whether you are in position to negotiate for a weekly instead of hourly rate)

if anything, knowing how much you can do (10 hrs of work) per week, makes it easier for you to propose how much value you can provide each week, no?
This is the most jarring thing to me when I land in the US. Already at the airport you see security staff, cleaning staff that look like they should have been enjoying retirement for several years.
Except that retirement age has been gradually extended, and there is no significant lobying effort by AARP or anyone else to go back in that trend.
This so disappointing to hear. I actually just clocked up 20 years with "the company" (my path was via DEC, Compaq, HP and now HPE). I mean HP was all about document printing and scanning, as well as massive storage and data lifecycle technology.
German here. Autocorrect is useless when using slang words or dialect, conjugations/grammar rules, and mixing languages (like adding in English words), which happens quite frequently
Author here - Uppy in a nutshell: no jQuery, JS-generated UI, easy uploads to S3 or the app, progress bar, resumable file uploads via tus.io, aware of encoding backends, react/redux support, form encapsulation, modular architecture, import from webcam/instagram/google drive support.
No, nuclear was never considered a renewable source of energy. It’s low-carbon, maybe.

Look, HN loves nuclear. You’ll find people here who propose running an actual nuclear reactor in every backyard. You’ll find excuses why the Sowjets were obviously incapable of securely running a nuclear plant, but capitalist societies can do it without a hitch. Then, two years later, you’ll have people telling you the Japanese were obviously incapable of running a nuclear plant, but France is obviously different. Plus, the Green conspiracy has somehow thwarted the breakthrough of cold fusion pebble bed thorium reactors, but now it’s just around the corner.

Meanwhile, in reality, it has simply become economically unfeasable to build nuclear reactors. Solar and wind are doing their version of Moore’s law. Batteries, and other storage technologies, are taking a bite out of the storage problem. Smart grids, combining load variability and harnessing end-user storage capacity for grid storage are basically there.

Your suggestion of throwing nuclear waste into the most corrosive substance available in quantity (sea water) nicely demonstrates this naïveté. There’s a post on the front page right now that shows that low-grade radioactive exposure has literally killed hundreds of thousands. Just because you can’t see it, and it was once the darling of science fiction, does not mean it won’t kill you.
> It is a weird, but entirely correct, assumption that all markets operate in a legal framework and are thus regulated.

I’m not sure what you’re saying here. Counter pint: drug markets.
See "oltp vs olap"
Open Source .NET three years later
A declining/aging population is problematic but a stable one isn’t. And you need some buffers in the system to manage the variations in birth rates. I thought this type of system was the most common these days?
Taken further, some people might want different "audio zones" in their vehicles[1].

I mean, sure, you could, but is sometimes having to compromise really so bad?

It feels like nowadays everything is about unique content tailored to our individual tastes, without compromise, and that we should each be sheltered from any experiences we might dislike. Or not be "engaged" by.

[1] https://m.phys.org/news/2013-05-speaker-cars-audio-zones-fro...
Except the US has positive average Gdp growth in the range of 2%, and positive population growth as well.
To squeeze every last dollar out of the available space becomes a quite literal meaning in this context. The ammount of the packages ariving damaged from the sheer load of each on top on another in the containers is really impressive, too.
What is the problem with nuclear energy? It is very safe and very clean and provides near limitless power. Our technology has been much advanced in the last decades. Now we would only need to build next generation (Thorium) reactors. At least that's what the hivemind here told me.
> The VA has also vowed the project would prevent death and disease from unsterilized equipment, a persistent problem at the VA.

Oh my god how is this a problem in the modern age? Stop wasting our money on gimmicks and focus on adopting modern hospital practices.
yeah... try building a nuclear power plant today for whatever the (heavily subsidized and long written-off) construction costs they use to arrive at those prices.
Ethical is a tricky issue here. Not everyone agrees that mere possession of such pictures is unethical.

This still leaves the obvious legal problems of having a database of illegal pictures, but we already let law enforcement do otherwise illegal things (including use of illegal pictures in some contexts)
Obviously it takes practice to become good. But if you practice something you have talent for you improve much faster than if you practice something you lack talent for.

I know this because I have tried both and can compare.
Very interesting,
What is the programming language used in the code examples ?
Do you have blog posts or other info on your kind of kayak design workflow? How did you join the maple boards? Were they just ribs and you put composite over it? There's a completely insane maker on youtube called Jamie Mantzel, he did some composite kayaks.

https://www.youtube.com/watch?v=n01cY3jOl0c
Western world has stopped having as many children, hence why governmemts are dead keen on immigration even though their constituents aren't.
https://www.verisart.com/about

"Verisart is the world's leading platform to certify and verify artworks and collectibles using the Bitcoin blockchain. Since its launch in 2015, Verisart has provided contemporary artists including Shepard Fairey with a free and easy way to generate permanent certificates of authenticity and reduce the scope for fraudulent activity. Verisart combines museum certification standards, distributed ledger technology and image recognition to its provenance and registry services. The company is headed by Robert Norton, formerly CEO & co-founder of Saatchi Online & Sedition Art and its Board Advisers include Peter Todd, Bitcoin core developer and Dr. Ahmed Elgammal, Professor of the Art & Artificial Intelligence Lab at Rutgers University."
That's Haskell. Slides 4 & 5 also hint at that, although it's easy to miss:

> Why Haskell is Interesting
Too bad that Germany uses coal to regulate wind power output fluctuations.

These record production peaks are sold as good things, but they are negative aspect of wind power. Only 20 percent or less of installed wind power capacity is available 90 percent of time.
Drivers obviously prefer a fixed route they know, but a complex routing algorithm could shift borders of neighborhoods or even interleave routes to meet timely appointments or size and weight constraints. Consider fairness, too, to equalize load.

Overall it seems the problem is fixed with brute force, taken to the extreme as in the topical article.
It depends on what you're doing. Certainly for standard design you won't be looking at Karnaugh maps. You trust the implementation tool to deal with the logic minimisation for you.

In certain cases say a piece of logic on the critical path that's preventing your design clock as fast as you want or something complex that you know requires lots of effort to meet your ppa (power, performance, area) targets, say an ALU or part of an FP unit, you'll be doing more manual optimisation. However even then you're probably not using a Karnaugh map, instead using more sophisticated techniques and then using specialised programs to do these for you (Espresso being the classic example https://en.m.wikipedia.org/wiki/Espresso_heuristic_logic_min...).

There's also things that simple logic minimisation doesn't take into account like fan out (how many things you have hanging off a particular output) and wire distance (a significant proportion of the clock cycle can be taken up in waiting for signals to propagate if the logic is spread across the chip). A hand crafted piece of logic is likely to use a mix of 'pure logic' type optimization techniques (that are simply trying to minimise logical operations) and the engineer using their judgment to work out what arrangement of minimised functions leads to the best implementation result.
Isn't lybia a dictatorship in both cases anyway ?
> Several countries outside the west are moving to do registration of land ownership on blockchain tech because their bureaucratic system is corrupt.

Speaking as a citizen of such country[1]. The problem is not in ledger/registry. The problem is that you can get a fake shareholders notice, a court order from corrupt judge, etc. and change the ownership in the registry. I don't see how blockchain solves anything that a simple publication of all changes wouldn't.

[1] https://www.bloomberg.com/news/articles/2017-10-03/ukraine-t...
Fwiw the last 10 years have an average yoy growth rate of 1.48%, which is a difference in magnitude great enough from 2% to point out.

We have population growth fueled by immigration. If not for this the US would have population decline. This is what makes us very much an outlier amongst developed nations [1]. Despite this our pension funding situation does not look good.

[1] Germany probably has strong net inflows of educated workforce from EU nations as well.
Sorry for being off-topic, but those slides are beautiful. Does anyone know what they're made in?
Honestly I hope most techies actually use the holidays for... holiday things. These are great and seem like a lot of fun! However, I can't imagine another industry where people take twelve days off to improve their work skills.
Nuclear weapons prevent war between nations wielding nuclear weapons. It did not prevent proxy wars.
Also because of nuclear weapons noone dares to intervene when countries like the US or Russia invade other countries (e.g. Iraq, Afghanistan, Ukraine). At worst they get a slap on the wrist from the UN, but they have permanent veto rights there too ¯\_(ツ)_/¯
Okay, time to repost: https://news.ycombinator.com/item?id=15998894
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Fleeing Google's Apps and iOS, Mandrake Linux Creator Launches 'eelo' Project
What are the major differences between (the latest versions of) Unix, Plan 9 and Inferno? Is there anything that Inferno and/or Plan 9 do that would be harder to achieve with a Unix-based system?
There was also that incident in Fukushima that turned the political tides against nuclear power.
The description of the tool does not say it directly, but it seems that the purpose of the tool is to increase security of your project during development and production.

But does it really improve the security?
Depends on the contry I guess, however for Central Europe that ranking seems just wrong. Java is likely to be #1 here. JS & PHP are much higher as well.
That sounds a lot like ”I need a gold star in my CV to boost my career”
I really dislike bitcoin (and most of the first-generation cryptocurrencies) for a lot of reasons (as an economist, I consider a deflationary, fixed-supply currency noxious). But the blockchain is rather fascinating. People seem to not realise what it is, generally speaking. And what it is, is this: a distributed manner of manufacturing scarcity of information in our day and age of infinitely duplicable data. It’s basically a distributed DRM system.
Ruby 2.0 Works Hard So You Can Be Lazy (2013)