skysight-inc/HackerNews huggingface dataset:
I understand your hypothesis that Cas9 only kills the cells that do not have the particular mutation and itself is a negative selection against the undesired genetic sequence. I do not believe the basic numbers and statistics of the rate of toxicity, size of genome, precision of mutation, etc. work out to permit that hypothesis, but it is a curious thought. As a hypothesis it is straightforward to test with a FACS-sorted population of cells expressing a fluorescent Cas9: does the fluorescent population die, or does the fluorescent population survive and contain the expected genetic mutation.

That experiment has been done and is actually written up here as a protocol to more effectively using Cas9. The paper clearly refutes the above hypothesis and demonstrates that modifications by Cas9 are generally proportional to the amount of expressed Cas9 up to the point of toxicity [1]. In it they tag Cas9 with a fluorophore, express the construct in cells and sort for those cells that express Cas9 (separating them from cells that do not), and binning the cells based on the level of Cas9 expression. Given your hypothesis, those fluorescent cells should die and not have the desired mutations, while the non-fluorescent cells will be enriched for particular mutations not found in the fluorescent cells. Some toxicity is shown at very high levels of Cas9 expression, but the on-target edits occur in cells expressing Cas9, and those cells live for a number of passages. See Figure 3 in the paper below:

> Cell population 1 displays GFP fluorescence intensities similar to those of nontransfected cells (autofluorescence level), whereas populations 2–6 display specific GFP levels ranging from low to high (varying ~500-fold). (e) 20,000 cells were isolated from each of populations 1–6 in (d) and cultured for 6 d, after which IDAA was used to determine indels at the KRAS locus in an aliquot of the cells. Note that editing levels increase with increasing GFP fluorescence level at the time of FACS and that almost complete editing was obtained at submaximal fluorescence levels... [T]he total number of live cells in each population analyzed in (e) was determined by trypan blue exclusion and expressed as % of population 1.

[1] https://www.nature.com/articles/nprot.2016.165

Given the above result, a significant amount of research is being done right now to preserve the useful properties of Cas9 - being able to home in on a particular genetic sequence - while engineering the protein to minimize and even prevent those deleterious cuts do DNA. And that research is finding steady success.
if you're getting your app finished this month.. maybe you shouldn't upgrade to 6..
And then if you circumvent that, perhaps bring charges under CFAA.
It's easy to dehumanize people when they live thousands of miles away, and are comparatively wealthy.
You mean the dick nipple?
It appears something about my existing profile was incompatible with the new version, causing a number of these strange issues. I didn't have a UserChrome.css, nor was I using a custom theme, so I don't know what it could have been, but creating a new profile at least dealt with the strange colour issues.

And thank you for that drag space tip. Definitely part of what I was looking for. I just assumed it toggled the ability to drag whitespace around while customizing!
> So you want to make them more rare so that people have a strong incentive to harvest from the wild?

No. I think them being illegal to posses is working to reduce field collection. This strategy is used for many other endangered plants (such as orchids) and seems to work. If people want to use psychoactives, then "magic" mushroom and morning glory seeds (or Hawaiian wood rose seeds) have a much lower impact on the environment and people should be steered towards those options by making them legal (most already are; carrot) and coming down on anyone who illegally collects Lophophora with a prison sentence (stick).

> You should look at the history of Chestnut blight in the US.

First of all, the species is not extinct. In fact, if you live in Silicon Valley, there is a chestnut orchard with some American chestnut trees just a short drive away in the Santa Cruz Mountains. There are many other populations and ongoing work to try and breed resistant trees.

Second, trees were cut down to create a biological equivalent of a fire break. The idea was, if there was a gap of 10-20 miles in front of the advancing disease front where trees were removed, then it might be possible to contain the disease. In hindsight, this might seem like a overly drastic measure, especially since it ended up only delaying the spread and not stopping it completely, but based on what was known about the disease at the time, and the huge importance of the American chestnut as an economic resource, it was a risk people were willing to take.

> Proactive regulation is not always the best move.

Yes, but sometimes it actually is the best move. I believe it is in this case.
Google didn't design the operating system? Microsoft didn't design computers either but they have been steering PC manufacturers for over 20 years since Windows 95 and the plug and play initiatives.
$5 fee for a $50 card? No thanks.
Well it's unscientific because there are no scientific studies as it's illegal.

There is also no agreement as to what constitutes microcoding afaik.
Node.js v9.2.0
I have one. Here is my next one: https://system76.com/cart/configure/lemu8
Wow what a rabbit hole you've sent me down. Check out this diagram of the jumping spiders (almost) completely 360 field of view.
https://en.wikipedia.org/wiki/Jumping_spider#/media/File:Jum...

Have you been able to breed them?
Just because there aren't studies on something does not mean it is not true. Science lags, and citizen scientists are left to explore and confer with each other to find what works and what does not. It is ignorant to ignore and belittle the progress in many fields that occurs before it is formally verified.
> that paranoia is pure cowardice

Sure.

> If someone in power cannot meet with someone one on one without attempting anything

Uh, that's not what the paranoia is. The paranoia is about accusations.
There won't be a bypass - Fx57 has completely dropped legacy extension support.

This is a good thing, and it's just a shame that so many addons haven't been updated yet.
It's odd that this was posted as I was thinking along these lines the other day.

I have a 2012 Retina 15". The first generation. I spec'd it out at the time hoping for it to last me at least a few years.

From 2015 I've been eyeing off every new release of Macbook Pro thinking "yeah this time I'll upgrade" but then I realise there is actually nothing wrong with my current one. Like, nothing at all.

I realise the battery is going die eventually and software updates will no longer be supported at some point, but for now it really is the best computer I've ever owned.

Note: I've been on all sides of the fence (Amiga/win95->winVista/OSX) so I'm not just a fanboi.
Until the day you open the wrong Word doc. Or click on the wrong link in email. Or run the wrong game.
CoreML doesn't actually support Tensorflow. It's support for Tensorflow is only through Keras which is fine if you just want to build stock standard models but if you're doing crazy research implementations then that's not going to work.
"The Human Genome Project is one of his most cited examples. After 15 years of work the team was at 1% completion. Critics were vocal about how it would take 100+ years to complete. Kurzweil predicted 7 years (7 Doublings of 1 is 128)."

When did he make this prediction? Was it before Craig Venter started working it? Can you quote exactly what he said at the time and provide a source?

Also, you originally said a "significant % of his predictions have come true within a ~15-year window". You've mentioned only single prediction. Unless he only made a few predictions, that's not a significant percentage.

"I interpret your question in response to my statements as you are not a fan because his predictions are not unique, is that what you are saying?"

I just have a severe allergy to people who hype themselves as much as Kurzweil does, especially when one of the main things they're known for -- the singularity idea -- came from someone else: Vernor Vinge. This just smells of charlatanism.

I'm open to being convinced otherwise, however. So if you've got more examples of Kurzeweil making long-term predictions well in advance of others and consistently being right, I'd love to hear them.

However, even if he is a good futurist, he'll still be a self-promoting copycat on the singularity idea he hyped to the heavens, and for which he made himself most famous.
Certainly I've seen compiler bugs.

1) Borland Turbo Pascal, where code would not execute correctly in the presence of a comment near the top of a loop. Deleting the comment caused the correct loop execution.

2) Texas Instruments DSP C-compiler sometimes failed to unroll loops correctly. Certain loop forms needed to be avoided as a result.

3) Recently I've been working on a C-compiler written in Golang, and the grammar definition was raw enough that it definitely had bugs. The bugs were fortunately obvious in that it would refuse to compile valid C code.

Of course, 99.98% bugs were mine.
I guess extension developers didnt expect being forced to WebExtemsions only so soon.
Australia's economy is a house of cards
I don't think whether that counts for racism is up for debate.
Well, I'm a Firefox user, not an extension developer, and I much prefer a performant browser with modern security features (and the ability to implement more in the future, which they couldn't do pre-57). I've lost a couple extensions during the move, but the tradeoff is well worth it to me.

And it sucks that you don't feel that way -- it really does -- but I suspect the vast majority of Firefox users feel as I do, and in the end Mozilla needs to cater to the most users possible.
Twitter's biggest mistake was hiring thousands of people when they only needed 30. It doesn't need thousands of people. It doesn't even need 100.

Twitter is not a technological marvel. It's something Oprah promoted, and Donald Trump bitches on.

And now, it's an API with a (social) class-based access, and without irony, also promotes itself as a leader in conversation and exchange.
Some places in the snow belt are so civilized the city plows your sidewalks and you just take care of the driveway. Easy peasy. No responsibility to the neighbors.
The foil seems to cost around 40 EUR. What would be the total price to have them apply it for you?
For me it's the Lenovo T61 with a 14.4" 4:3 display (or any of the older 4:3 IBMs).

Yes, compared to modern laptops it is heavy, slow, bulky and has a shitty display but: i) I can replace every single piece of it with a simple screwdriver and ii) 4:3 is still the best aspect ratio for reading and developing. If I am doing the latter I prefer a screen with a lot of height. A 4:3 aspect ratio gives me a tall screen while keeping the overall size of the laptop down.

When DVDs became popular there was suddenly the idea that a laptop _had_ to have a 16:9 aspect ratio (e.g. "HD") and unfortunately this killed the whole idea of a laptop with a tall but narrow screen. Microsoft seems to go back to an older style with their 3:2 but I'd still love to see a modern T61 with a true 4:3 screen.
OK, but if the information is obviously bogus will advertisers keep paying? I don't think deleting fake accounts is necessarily an easy problem. Particularly when we consider that a false positive is much worse than a false negative as far as user impact goes.
Perhaps it's as simple as that. I dumped too many resources into my last effort: time designing, flying out, sitting down with their dev advocates, all a waste of my time. I shan't reward them for pulling the rug out, or make the same mistake again.

Also, it's in solidarity with developers who invested even more than myself, years of time into apps, to be betrayed.

That's an emphatic "hell no" from me dog.
It also has toolbar RSS feed support. I've been using this since when Firefox was in beta (Phoenix) and it's a killer feature for me. I may use Feedly on iOS but desktop/laptop I love having the same feeds right on the toolbar.
Chrome doesn't support RSS feeds in this way because there's no way to advertise with them.

Always watch where the incentives run, Chrome is for Google's best interests, not the web's and not the user's. Always has been and always will be because it's a for-profit organization.
Take a look at the eff's privacy badger add on, which let's you control each script/source on a per-page level
I know someone who has made money with a simple ETH trading bot. Apparently it can be done.
Yeah I'm sure the guy who was immediately itching to do an interview with all the luminaries of the alt-right was a shrinking wallflower who didn't want to draw any attention to his manifesto or himself.
except for the old android I don't see any problems with my S4, so I'll be using it for another 2 years. And I actually prefer that size, it's easy to use with one hand
> oh, there it is, 25 lines beginning at line 8201.

This comment made me laugh with the tragedy that is the modern web.
@ has always had another meaning in irc. People who use @ to refer to people are immediately identified as n00bs.
I think that's a great point: it's difficult, near impossible, to make any particular piece of software contain 100% of the features that 100% of people want. Especially in the case of a tiny-minority feature, a specialized version that caters to your needs sometimes makes the most sense.

I just worry that people using some of these alternative browsers are opening themselves up to security issues since those code bases are less well-tested for security problems, and often they don't have the developer resources to implement things like process isolation and sandboxing.
Do people actually use phone-based VR for anything? I got to try one of the Google phones in VR mode for a few minutes and was... thoroughly underwhelmed. It was ugly and unconvincing enough, and so low on interactivity (necessarily, best I could tell, having no input device other than the phone itself) that I can't imagine actually using it on a regular basis, and I'm the guy with a 10-year-old TV and similar-vintage monitor, so my standards aren't exactly high. I'd probably sell or throw away one of the headsets, given it for free.

Do people actually use them much? And if so, what for?
I got offered a job there. Then another HR employee called and said wait, they needed to find out my SAT/ACT scores before they could actually offer me the job. I had most of a master's degree at that point. I still got the offer, but it was really strange.
I don't think this is "always" true. The power of having the data in records is that you can trivially pivot and slice and dice the data in different ways. When it's aggregated to a count - I don't have that ability. When trying to debug something that's happening, I find it far easier to have the entire records.

As for scaling, it scales very well (you can read through the elastic blog/use cases to see plenty of examples.) That's not to say there aren't levels of scaling it won't handle. But I would venture to say that for 99% of the people out there, it will solve there problems very well.
>"I understand your hypothesis that Cas9 only kills the cells that do not have the particular mutation"

The hypothesis is that it is more likely to kill those cells than the others, and does so at a high enough rate to select for cells missing the target site. Other things such as NHEJ/HDR happen too.

Anyway, the technique in the paper you linked is not supposed to be causing double strand breaks, which for a long time before CRISPR/Cas-9 were thought to result in Death >> NHEJ >> HDR. This is a totally different scenario, but it is quite odd they do not mention toxicity at all since that is a major concern of these technologies.

I'll check the Lonowski et al paper and get back to you.

EDIT:

> "Given your hypothesis, those fluorescent cells should die and not have the desired mutations, while the non-fluorescent cells will be enriched for particular mutations not found in the fluorescent cells"

Perhaps there is a typo here but this is exactly the opposite of what my hypothesis predicts. The surviving cells should contain cas-9 and the mutation. Cas-9 damages the DNA of some cells less because the the mutation was already there. The cells with no cas-9 are noise because they were never treated.
Whoo. It has VGA output.
There are people talking about it, but there’s enough profit and personal identity at stake that it’s hard to have real society-wide conversations about what we really want to value as a society.

There exist countries where automobile deaths are not considered acceptable, or where not all policy planning takes an ironclad need for automobiles as the exclusive mode of transportation as a basic premise.
The smallest nuclear plant in the US generates 582 MW of power with a capacity factor of 92.1%. That capacity factor means that in a year you'll get 582MW * 9258 hours per year * 92.1% = 4.9Trillion kwh/year of power. That's how much we need to replace if we chose to shut down the SMALLEST nuclear plant in the US (the largest is almost 4,000 MW).

The largest wind turbine being designed anywhere in the world right now would be 500 meters tall (taller than the empire state building) with 200 meter blades and at peak output would generate 50MW. Capacity factors for on-shore plants in the US average about 33%. So 50MW * 9258 hours per year * 33% = 0.152T KWh/year. The largest turbine currently built in the world gets 8MW not 50MW so 0.024T kwh/year.

I.e. we would need ~200 of the biggest wind turbines ever built to equal the amount of energy we get out of the smallest nuclear reactor currently running. And that is just to break even. Comparing the median nuclear plant to the median wind turbine makes the numbers even worse.

Hydro has the problem in the US that there aren't any good sites left. Solar costs even more per KW or per KWh than wind does (~1.5x is what I've seen at grid scale in the US) especially when you include the storage needed to maintain consistent supply throughout a day (~3x wind)
We've found compiler errors frequently. An absolute hot-spot for compiler errors for us was (relatively) obscure platforms, so we found SDKs for embedded MIPS processors that broke basic gcc intrinsics. No-one had ever noticed that really basic intrinsics (at the level of popcount/count leading zeros) were wrong.

C++ was another hotspot of brokenness for early gcc 4.x.
You can deploy TensorFlow model binaries as serverless APIs on Google Cloud ML Engine [1]. But I would also be interested in seeing a TensorFlow Lite implementation.

[1] https://cloud.google.com/ml-engine/docs/deploying-models

Disclaimer: I work for Google Cloud.
Is the party in this story lacking empathy the media? Or the guy who wrote a manifesto about how women just aren't fit for jobs as engineers, genetically?
On Gnome with Wayland, it works, but only with integer multiples. Gnome 3.28 (which will ship in F28) will have full fractional scaling support if you need that.
Yudkowsky is another self-promoting hype machine, in the vein of Kurzweil. I wouldn't take him as the most unbiased source on the history of the singularity idea or how "his" ideas differ from the rest.
I knew what laptop Marco would be talking about before I clicked on the link. Perhaps that says something about Marco, but I have the feeling it's almost certainly about how widely-lauded the old rMBP was, and still clearly is.

Apple got so many things right with it, because all the design choices made sense, for exactly the reasons Marco gives.

The design choices being made at the top of Apple no longer make sense. Take the touch-bar, for example; it was a passable idea, but flawed in execution and production. If you want another example, how about DongleGate™? The most recent USB-C trend was not handled the way Apple used to handle standards progressions. Yes, they annoyed us in the past with too many FireWire ports, and an overly-futureproofed candy drop iMac (USB)... But even then, those choices made sense because the rest of the ecosystem was thought about. This is clear when you compare it to today's offering, where you cannot plug a new iPhone into a new MacBook, out of the box! Incredible...
Uber dealt with class action lawsuit alleging assault and rape by drivers
I wish they went better than 1080p screen. Everything else seems to be on point, including price.
I've had some success using portia[1]. Its a visual wrapper over scrapy, but is actually quite useful.

https://github.com/scrapinghub/portia
..oh. elsevier.

https://www.ncbi.nlm.nih.gov/pubmed/29102613
Intel ME is a processor that runs the MINIX operating system. It's the operating system that connects to the network. AMT is a module that is run in the operating system, that uses the network functionality. Although no one really knows for sure, as it's completely closed source and very little "technical" documentation is provided about it.
I haven't downvotes your answer, but you ask why people voted you down… so here goes.

It is because you try to pin everything on Socialism, when the real problem is actually Cronyism and Despotism. I.e. it was their political system, not their financial system, that did them in.

There are plenty of countries with various degrees of socialism that are not bankrupt. Australia, for example, had a socialist, very left leaning, government that gave many of the same things out. But these countries generally have well established democratic institutions. And it is these institutions that allow a country to change direction if it makes a mistake.

Venezuela on the other hand does not have a strong democratic institution, and so has no real way to escape the mess that it created. Its leaders are invested in the solution that they chose, and dug ever deeper in to show how right they were.
Personal identity is nothing but a convenient mental construct, imo. You're not the same matter you were at birth, nor can you make a good argument as to why you shouldn't be considered a direct physical continuation of your mother. It's practical to think of one as having a unique self that's distinct from its environment, but that doesn't reflect some deeper truth, it's just a framework for reasoning about the behavior of some arbitrary subset of the ridiculously complex system that is human society embedded in the more ridiculously complex system that is life embedded in the more ridiculously complex system that is the universe. Don't expect naive conceptions of self to survive that far into a future in which things like "brain uploading" becomes possible.
Honestly I can't tell... I do see them being sold, maybe as a trap gift for grandparents.

I've heard of people using it to watch YouTube or Netflix as a replacement for a big TV (most people have a phone but not everyone has a big TV in his room here).
Again, is it so hard to believe that people are different and some people like to work?

There are real cults out there, Amazon isn't one of them.
Is telling someone "hey don't have so many kids, it's bad for the environment" bad? I think you're mistakenly throwing a hidden "by force" in there.

Nobody is forcing African girls to have fewer children. If you'd like to discuss, I'd love to hear your argument on why you think that giving African girls more choices than "bear four kids by the time you're eighteen" is bad.
I agree. I have the 2012 model, bought new in 2013 for cheap. 8 gigs of ram with an i7. I recently had the battery replaced. I has some blemishes on the screen. Every ones in a while I'm looking at upgrading to a Mac Pro, but then again, this little laptop is still going strong, so why should I?
Open a resume.
Browse from a coffee shop.
Click on a link from your bank.
Use your phone while on the subway.
Play music that you paid for.
Cross an international border.