skysight-inc/HackerNews huggingface dataset:
The simple explanation is that they are different products that only overlap to a limited degree.

If you're doing a standard RNA-seq experiment, short 100bp to 200bp Illumina reads are just dandy for what you need. Most of the time, at least; you could, for example, be interested in different splice variants for a gene family; in that case longer continuous reads are valuable. But then it's no longer a standard RNA-seq.

PacBio and pore-type sequencing is most valuable for polishing reference genome assemblies. For instance, this is a huge issue for crop plants because they have huge, repetitive genomes and are often polyploid. So there's a lot of interest in creating high quality assemblies not just for e.g. corn, but the major research inbreds (B73, Mo17, etc.) as well as private breeding lines. Some of the most valuable crop QTLs (R genes, microRNAs, transposon fragments, etc.) are just 'junk' sequence that plays a regulatory or defense role, so it's super important.

In general, the Illumina data is very high quality and very inexpensive, and works beautifully provided you have a high-quality assembly to work with, and what you're interested in isn't very repetitive. It has many good applications, so I don't see much risk at this point.

If, at some point, longer reads can be done with high fidelity and with similar cost, then there would be an issue. I don't think that's going to happen anytime soon, but I'm sure Illumina is looking at that long-term.
Could this be made difficult by obfuscating some sensitive part production through US made black box that stay controlled at the factory. No tesla black box, no nice tesla part oob, head start kept ?
Maybe they're eating their own lunch. I'd bet if the metric is revenue-per-employee, 37Signals is probably kicking most of the bigger tech companies arses.
why? that screams "bad devops" to me
Why should a person have any control over arbitrary construction happening in their neighborhood?
Modern capitalism can be easily sustainable. You just have to do two things.

1) Legislate frameworks for market based solutions to externalities i.e. tragedies of the commons such as air pollution, water quality, etc. Cap and trade would be one framework for addressing CO2.

2) Eliminate mercantilism, where government policies are used to interfere in market forces, usually for the benefit of wealthy business owners. I.e no more subsidies, of any business, no more "incentives" to overbuild, overproduce, etc.

Boom done. For those of you who say yea, but it's impossible that those 2 things could ever be legislated successfully, I say, yea, your right. But that doesn't mean we can't acknowledge the solution. And for those of you who say, but capitalism bad! I say, yea but those two things are just as impossible to implement under socialism, communism, marxism or any other system you can devise.

In reality, the only solution is to have a Julius Caesar conquer the known world, declare himself or herself dictator for life, and then say, you idiots, we need to have a sustainable planet, and just implement those changes by edict.

Unfortunately the new Caesar would then drop by the senate for a session, and senators backed by the dozen biggest corporate welfare recipients would stab him or her to death. And the senate would immediately repeal the new laws.
They're not even minute differences. One example toggles between two pictures of an airplane, with the second having the engines photoshopped out. Just a one-second delay is enough to make it difficult to notice what changed.

Now of course it's easy if the two pictures (or voices) are completely different. But who would be suspicious if the two voices sounded similar?
What do the most successful freelancers know that you don’t?
Need advice from a Hardware Startup
HARDWARE ADVICE: Do you use a software platform to manage the procurement of your hardware BOM?
I have a Paw license and recently I started using Insomnia instead: https://insomnia.rest/

I find Paw's project management very annoying. It assumes that I am working on some project and wants me to organise everything like that. Fine, lets do it, so where are my projects? They are hidden in some dropdown that you can have fun finding.

Insomnia is much cleaner and more to the point.
As Robert Kennedy said in 1968:

Too much and for too long, we seemed to have surrendered personal excellence and community values in the mere accumulation of material things. Our Gross National Product, now, is over $800 billion dollars a year, but that Gross National Product - if we judge the United States of America by that - that Gross National Product counts air pollution and cigarette advertising, and ambulances to clear our highways of carnage.

It counts special locks for our doors and the jails for the people who break them. It counts the destruction of the redwood and the loss of our natural wonder in chaotic sprawl.

It counts napalm and counts nuclear warheads and armored cars for the police to fight the riots in our cities. It counts Whitman's rifle and Speck's knife, and the television programs which glorify violence in order to sell toys to our children.

Yet the gross national product does not allow for the health of our children, the quality of their education or the joy of their play. It does not include the beauty of our poetry or the strength of our marriages, the intelligence of our public debate or the integrity of our public officials.

It measures neither our wit nor our courage, neither our wisdom nor our learning, neither our compassion nor our devotion to our country, it measures everything in short, except that which makes life worthwhile.

And it can tell us everything about America except why we are proud that we are Americans.

https://www.theguardian.com/news/datablog/2012/may/24/robert...
What's indirect about the link between money supply and prices?
A similar idea is the muon g-2 magnetic moment measurement: https://phys.org/news/2017-06-muon-g-begun-phantom-particles... It goes back to 1959, with ever more accurate measurements: http://library.cern/archives/CERN_archive/guide/experimental...
Are you sure? Have you looked at population growth vs. housing growth in those areas, or how restrictive the zoning is?
> humans love to copulate and take pride in having children.

Developed countries are actually experiencing a population decline. As people get wealthier they tend to have fewer children, in some places less than 2 (replacement level).

"All wealth is material in nature" ignores centuries of intellectual property.
It's a she.
I'm all for it if someone has a good way of going about making it happen. But let's play fair... We can't appeal to the western tradition of societal fairness of that kind. It hasn't existed.

It's fair enough to want it to become a part of it, but it has to be invented first.
That argument simply assumes its conclusion by arbitrarily labelling hands as 'external'.

The 'existence' of our hands is perceived by our senses and filtered through consciousness. There is no way to prove that they actually exist outside of our minds.
This is a great point. I don't know why we aren't running the linter via the CI
The amount of times insect populations are sampled (sparingly) means that there's simply not enough deaths proportional to the population to have a meaninful effect on selecting for those traits.
Your hot dog stand was the growth and now they need a 10% bigger next investment for their return on it. They need you to have a competitor with a better stand so they can loan you both money or they need prices to have gone up thanks to other Banks finding speculations and you wanting to sell. Otherwise, they now have 10% more money they need to invest and less of a solution than 2 years ago.
Wow. That makes sense. That explains why so many seemingly infrequently visited businesses still have storefronts in Berkeley. I always wonder how they stay in business. I thought perhaps commercial rent control.
Interesting, I haven't tried it on the newest chemistry but last time I did a flowcell (earlier this year) that was the INDEL error rate we achieve.

Substitution error rate was very low, but you should evaluate INDELs specifically, rather than just identity like you did on your git, because it is the major drawback of this technology.
I run bandgap.io off of protonmail. I have had no problems with deliverability, have had great interactions with customer support, and enjoy using the product. After email hack after hack, I just couldn't see how I could ethically store my own users support emails unless they were end-to-end encrypted.

Potential improvements: Searching the inbox isn't great, and I'd like to reduce my attack surface by moving my transactional email from sendgrid to protonmail (not yet supported). Also, it might make sense for protonmail to become an OAuth provider-I'd be willing to support it on bandgap.io
There are no observations that are not products of consciousness, so there are no prior observations in this case.
Yeah, and maybe that's part of the issue right now. There is only a tacit agreement on using public style guides
And then you have to find someone willing to rent a $4K/mo property to four college kids...
nothing is neither created nor destroyed, by that very law there cannot be a crop that does not take from the soil. If I eat a banana that has potassium in it, it lends to the fact that it took potassium out of the soil.

farmers do add fertilizer and other stuff back to the soil, though we are finding that maybe there is more to the equation. certain methods like tilling are done to kill parasites, etc, but it may also make the soil vulnerable to erosion. luckily engineers and farmers are looking at these things more closely now.

its also important to note this is just 1 study. just 1 region. 1 particular type of trap. there is still lots to know / find out, but I think its wrong to be jumping to huge conclusions just yet.
This is technically true, but while in office, Chavez eliminated most of the institutions necessary for a healthy democracy (eg independent judiciary). If he was not technically a dictator, he acted like one, and his successor is filling the role he made possible.
Ok, but so what? That doesn’t explain why anything else makes more sense.
Making viral tools, not viral blog posts, to grow a 7-figure SaaS company
no one in JS land gives a crap about riotjs though
One day I was listening to an episode of Radiolab[1] (an NPR podcast) and I was surprised to hear they were collaborating with Buzzfeed. For the most part I still think of them as a listicles site but I guess they are trying to break into more serious journalism.

1. http://www.radiolab.org/story/60-words/
I have the newer stiffer yellowing one that degrades and literally falls apart in pieces. I think they cheaped out on materials or something.
This is a great story, a fun deconstruction of the QR format and a nice bit of hacking to "zoom and enhance" their way to a private key.
I grew up in the Bay Area and got a feel for the tech sector up there. People always throw around the "network" argument out there for why they decided to move up. Truth is you can get just as much networking in if you move to an emerging tech hub along with positioning yourself better by more easily establishing yourself/buying cheaper property. I'm looking at you Portland, San Diego, and Denver.
For quick-and-dirty things I use cURL or httpie otherwise I use Paw (and have for about a year now). I had previously used Postman before that, but even then Paw was a better app—native app so it looked better + felt faster, nicer collections, better default shortcuts, etc.

Paw has a lot of power behind it, otherwise if you're just doing basic POST requests, stick to httpie. The code generators and extensions are nice. Easily switching between environments (e.g. dev, staging, prod). History of run requests. Tying values from other requests into other requests—e.g. get an auth token from a login request, and use that in a header in all other requests. I still only use a fairly basic amount of the features too. It's a very polished app, and the devs seem good too.
So I used to feel like you do until I moved to the Santa Cruz mountains. A half ton pickup is pretty much required. I have a chevy extended cab, 8 foot bed, gets used all the time for stuff that I couldn't do with a car.

In addition to that I have a chevy 3500 HD dually flatbed that gets used as well. It's an entirely different beast, I've hauled 10,080 pounds of base rock in that thing and I still had spring left.

It also gets used for patching potholes:

http://www.mercurynews.com/2017/03/17/mountain-vigilantes-ta...

The half ton is the blue truck and the 3500 is the white truck. This is set up as a quick attack wild land fire truck (there is a 425 gallon water thank, 9HP davey fire pump with 2 hose connections, 300 feet of 1.5" hose, bunch of nozzles, shovels, etc):

http://www.mcvoy.com/lm/fire-attack/

My trucks get used pretty much daily, living up here without them makes zero sense to me. They are just tools and very much needed in this environment. Sort of like guns, I lived in San Francisco and was very anti gun, moved up here and realized that guns are just tools and sometimes you need them.

That said, I'd love to replace the trucks with electric versions. I'm a huge fan for a lot of reasons. Tesla, if you are listening, please, please give us a family of pickups. If you do just one then a half ton is gonna be the highest volume but I'd love a beefy 1 ton.
This is a great example of how we don't weigh externalities in GDP, why we should, and why we need market based solutions to those externalities. For example, a cap on river pollutants so low the river and wildlife can't be poisoned and each company has to buy rights from the limited pool, or if too expensive, find ways to limit their pollutants.

I once read (from Milton Friedman IIRC) that some european countries passed a law to limit river pollution by requiring companies to have their river water intakes downstream of their outflows. Supposedly this made a huge difference in the amount of pollutants they dumped in the river. That is a simple but limited way of imposing the externality costs on the persons who inflict the damage.
Tons of games in that era had dense fog for that reason.
I was not, actually. That's why I said it was a bad analogy.
Key takeaway, structure is emerging whether or not you want it to, so take care to put in place a minimum viable structure that is malleable. One such list of properties of what she considers an effective structure is proposed at the bottom and is worth a read. Directly applicable to modern organizations and not just a historical essay from the earlyish days of the women's liberation movement
> So, you're telling me that the $1MM home (which will be roughly $5600/mth) will give you nearly $20k in tax deductions

Something like that.

Federally, local property taxes and mortgage interest on the first $1 million of principle for first and second mortgages for your primary residence are deductible from your income. For California, the rules are slightly different, but I think that they are close enough to use the same rules for estimation purposes.

Using the interest rates from your example that would be a deduction of 4.5% * $800k + 6.5% * $100k = $39.7k for the mortgage interest deduction and roughly $12.5k for the property tax deduction for a total deduction of $52.2k. If you can afford a million dollar house, you are probably in either the 28% bracket or the 33% bracket for federal taxes and in the 9.3% bracket for California taxes so that will save you $14.6k-$17.2k on federal taxes and $4.9k on California taxes.
The Mutual Exclusion Problem Has Been Solved [pdf]
Cool idea, thanks!
It's not a monopoly though, this is simply the inevitable conclusion to any price competition: suppliers will be pressed to cut costs in ways that lose American jobs. Having a minimum wage means other countries can undercut our labor and pass some of the savings on to the retailers, who then compete more efficiently vs those who do not put pressure on suppliers. There are many legitimate reasons to criticize Walmart, this one is not one of those.
This article and whoever wrote it just ... sucks
Thanks, you’re right. I wasn’t aware of that. The experience is rather crappy though. Cumbersome to scroll on a smartphone screen and I’d love to see to entire statement not parts of only at a time.
Donuts and KFC are terrible. You should stick to Ben & Jerry's.
Show HN: BLK STACK; the anti framework, framework
It sounds to me like the Crazy Eddie scam from the late eighties. While securing financing from Wall Street, Crazy Eddie took the bankers on tours of its various warehouses in New York. And had workers populate the next warehouse to be visited with inventory from one of the already visited locations, making their inventory physically apppear to be 2-3x as large as it was in reality. Enough to make the valuation lies told by their books look true.
Won't someone please think of those poor, helpless brands?
I've only sequenced a few samples on a MinION. The dominant error seemed to be miscounting the length of a homopolymer stretch (e.g. CCC->CCCCC) but the substitution error rate was also clearly worse than Illumina. I've gotten used to less than 0.1% error from DNA sequencing on a HiSeq 2500. My guess is that the MinION data had 1-3% substitution errors.
Just to be clear this isn't my analysis - and yes most of the errors here are due to indels.
Show HN: Tiimebombs
I had a trial and found the selection for developers wasn't worth the $10.

There are a few little utilities, like the Regex tester, that are definitely neat and well made, but given the once a month I actually need that functionality, I'll just use a crappy web app for it.

Most of the software I found to be the 3rd best in its category (or worse), for example, It doesn't have OmniFocus or Things, it has a bunch of to-do list apps I've never heard of that all have poor design and UX. Besides that there's a lot of the soft of system enhancer software that I thought made my computer go faster when I was younger but that I've now realised is mostly pointless.

For me to resubscribe to SetApp, I'd want to see best-in-class apps like Things, OmniFocus, SublimeText (or even BBEdit, I'm not a fan, but it's a good Mac app), RapidWeaver (again, not for me, but good for many), Transmit, Coda, Delicious Library, etc. I'd probably pay more than $10 for that sort of selection.
Because Google love removing everything for no reason, and they love it. There are lot of features that are being removed from Google's services for no reason. Example: Dark mode removed from Android OS without notice. And they just bring us annoying features instead. Google will die soon at 2030, they have no live, trust me.
Right, but the contrast range 1:1,000,000 - that's a real range isn't it? Not an integral one? So why does it ideally need to be represented with a particular quantisation of 1,000,000 different states? I mean there's not 1,000,000 different values between the real numbers 1 and 1,000,000 are there? There's an infinite number. There's only 1,000,000 values (20 bits) if you decide to quantise on each integer. Why do you need to do that? Why not more? Or less? What's special about the integral values on the number line?
For metadata protecting applications I would check out ricochet.im, briar, and i2pbote.
Commuting to collaborate with other people face-to-face for half the day.

How is this inefficient, unsustainable?

It provides the combination of private work and group interactions that are parcel of most white collar jobs. The vehicles will be electric, and many will probably spending any idle time at solar farms, to save on energy distribution costs, if they aren't being used as private offices. It is going to allow people to live where the want, and still do they work they want to.
Show HN: A yield curve application made with quantra.io
This may sound strange, but I'm sometimes grateful that, when I was a wee lad, Internet wasn't invented and computers took up whole rooms. I think I'd have wasted even more time than I did.

It means I read a lot of great books, for example. I used to read them and then write about them. I'd spend almost as much time writing and thinking as I did reading the books. I'd make notes, quotes, and tie things in with other literary works.

If I'd had computers and the Internet, I'd have spent that time watching porn and posting fart jokes.
Any properly functioning bureaucracy institutes performance improvement plans so that there will be a paper trail for firings. Indeed any not-entirely-dysfunctional bureaucracy has this. Also, being on a performance improvement plan means they want to fire you. Sometimes this doesn't happen -- I know a guy who was subsequently elevated to middle management after having been on a PIP as a junior employee, but it delayed his advancement by at least 5 years.

Edit: typo
As long as enough high-rise apartments actually get built somewhere, this is fine. The problem is, the NIMBYs are broadly distributed, and tend to fight such construction everywhere.
The natural result of having borders? If you think people should be able to live wherever they want then where do you draw the line? Can I come live in your house and sleep in your bed whenever I want?
I get the point of not hanging on to mediocrity but I don't think the term "losers" is what's most appropriate here. It's too reductionist.
Ask HN: USA-based SaaS and Candian payments?
I am bootstrapping a SaaS company and we have our first customer. We're based in the USA, they are in Canada. We have a year of checks to deposit for the monthly fee. This is fine but I have to go into the bank and deposit via a teller due to CAD funds. Our monthly fee per customer is $650-1300 USD.

Is there an easier way to accept payments from Canadian customers? We do have a business banking account with Chase in the USA. I see options like TransferWise.com and it actually comes out cheaper than Chase due to the exchange rate being more favorable (even with the 1% fee). But it doesn't look like it is setup for recurring payments. I'll go ask them. We could use something like Stripe but the fees are higher and I don't know if our customers want to pay by credit card (I don't know B2B payment conventions in Cananda).

I am asking here to possibly get advice/suggestions based on experience as there appear to be a lot of options.
Yes, the execution when it comes to self promotion is very important. No association, but I really like this answer[0] from CloudSponge, saying why their product is best on a thread asking for alternatives. What could have been very slimy comes across as genuine and helpful. It nearly converted me to a CloudSponge user when I came across the question while looking for alternatives.

[0] https://www.quora.com/What-are-the-alternatives-to-CloudSpon...
When driving through south bay I noticed several buildings with red rope in place of where they would have another story or two on the building. Turns out the bay area has very strick rules around building too tall and disrupting flight patterns of birds. Probably one of many reasons they haven't built massively upward.
> ... don't know where it could be used practically.

Maybe with read only databases?
> ...vast majority of the electorate that's socially liberal and financially conservative...

There isn't a vast majority on controversial social issues or they wouldn't be so controversial.
That's what all the college grads I knew at Google 2016-2017 were making, probably closer to $200k unless they started trimming the RSU grants because of $GOOG appreciation.
It always seemed insanely stupid to me every time I read something about people claiming certain genes being "non-important" or "non-functional"

We have about a 0.0001% comprehension of this whole system, how can you possibly be so arrogant as to think you KNOW that certain parts of the sequence are not important or used in _any_ way? Then again, I'm definitely not qualified to comment on the topic.