skysight-inc/HackerNews huggingface dataset:
> "Co-housed FVB/NJ mice without CRISPR-mediated correction were used as the functional-deficient control. Briefly, an sgRNAexpressing plasmid had been coinjected, into FVB/NJ zygotes, with the single-stranded oligodeoxynucleotide (ssODN) donor template and Cas9 protein to generate mosaic F0 founders.1"

Following to reference 1:

> "The sgRNA plasmid was co-injected with the single-stranded oligodeoxynucleotide (ssODN) donor template and the Cas9 protein into FVB/N zygotes to generate eleven F0 founders.

[...]

Double-strand breaks (DSB) were detected in 7 of 11 mice

[...]

The target region was sequenced, revealing that F0 3 and 5 incorporated the donor template precisely in 35.7% and 18.8% of somatic cells, respectively (Fig. 1c), while F0 7 and 8 incorporated indels in the integration, corroborating the unexpected results in the RFLP data.

[...]

A mixture of 3 ng/mL sgRNA plasmid, 3ng/mL of Cas9 protein (NEB Ipswich, MA), and 1mM ssODN (Integrated DNA Technologies, Iowa) was injected into the pronuclei and cytoplasm of FVB/N inbred zygotes. Zygotes that survived injection were transferred into oviducts of 0.5-day post-coitum, pseudopregnant B6xCBA F1 females and carried to term. The resulting gene-corrected mice were backcrossed, initially into the FVB/N background, to determine the germ-line transmission efficiency of the repair."
https://www.ncbi.nlm.nih.gov/pubmed/27203441

This is missing some crucial info isn't it? How many FVB/N zygotes were injected to generate those 11 original mice?
You read even better than me! In retrospect, I guess it's intuitive that temp jobs are the most likely to expand and contract in response to recessions.
Sure, but then that's not anything new. Any persistent-world multiplayer game would qualify, and if you want to focus on real-world locations, then there's at least Ingress.

Also, last time I checked, Pokémon spawning wasn't synchronized. There's close to zero shared world state between the player (only the static - spawn zones, Pokéstop locations, Gym location and status - and lure).

Anyway, to be clear: I'm not complaining about the game itself - just about abusing the term "AR" to describe it.
There's a Dennis Rodman in every line of work. Just because his NBA career came to a complete end in 2000 didn't stop him from finding one more chance playing basketball in Mexico . . . or the Philippines, etc. Not to mention lingerie football.
>those of us complaining are using them for gaming, or serious compute jobs.

Then... don't do that? Seriously, they're not intended for this. Are you also upset that your $50 Ryobi drill doesn't have enough torque to bolt together a 747?
If anything, there aren't enough C-level folk. Low supply is why their wages are so inflated, we should be allowing H1B for CXO's.
“Try Before You Buy” Job Hunt: On-Demand W2 Employment
How do you return a downloaded file without copying it?
I'd expect the same. But I'd bet a majority of the individual actors, including those making the final decision, would really like to be able to take that vindictive option.
The FreeBSD port of Docker was unfortunately never merged, and needs completely reworking since it was written for the old monolithic Docker. There is some interest but no one actually working on it and I have been doing some portability fixes to containerd now and then but it needs some sustained effort.
>AI which is about replacing the mind

Give me a break. AI replacing the mind... Put zebra stripes on an upside down sofa and the best image recognition algorithms see a zebra. That's how far the "understanding" of our AI goes. What you are doing is fearmongering.
Given that it's an article on BBC I expected at least a mention of the rock opera Tommy[1] whose protagonist is an autistic pinball wizard.

[1] https://en.wikipedia.org/wiki/Tommy_(album)
Get Free Security Audit If You Replace Passwords
I came up with that technique in middle school geometry. I demonstrated it on paper and it worked! I showed it to my teacher and she thought it was cool, and it didn't go any farther. I don't know if she didn't know it was impossible, or didn't get it, or what, but in any case I don't blame her. Of course my demonstration was with a fairly small angle, so it looked right. Much later I realized that it obviously failed with large angles as you mention.
Ask HN: Do you need an angle/USP to start a successful business?
Is a unique selling proposition absolutely necessary to create a successful business? if so, why or why not?
You'd be surprised with the many advantages webview apps have for specific use cases. Often, the client doesn't know what is needed, but it has to be ready by tomorrow and it might change before next week. Also, the app has to be on both stores before any real content is ready. And it needs to match the look of their website.
> This classes in universities is a scam: instead of teaching people of real world things, they teach how to work with yandex tech stack.

Is that true? The sample curriculum [0] doesn't seem to support that.

[0] https://yandexdataschool.com/edu-process/program/data-analys...
The scope of the Single Market is broader than that - it includes standardization of weights and measures, standardization of product names, etc. - rules that apply to private companies, and are meant to break down extra-legal obstacles to trade.
Thanks. I actually wrote the comment, because of :

> This enables writing code using async and await at all times, which is what any sane developer would do when writing code for Node.js.
We were talking about this just days ago: https://news.ycombinator.com/item?id=14386626 (not that we're done, just merely providing a link for others)
While a car sitting alone on a shelf is not constructive, there's nothing that prevents the car from becoming a component of a collection of toys that become constructive when put together.

I used to build roads, buildings, garages, towns... I'd set up small construction sites, and even try to engineer my own traffic intersections. You can set up hot wheels tracks, and learn about physics. I learned how to take apart the cars and swap out parts on them. Many of the cars became just another Lego as well.

Sometimes the cars themselves are what would inspire me to build a structure to support them out of Legos or Lincoln Logs, etc.
Possibly because people with more time than responsibilities to fill that time often end up stirring things up, especially when in close proximity to each other. Just look at college. Society does place a premium on stability, so too much of this might not be a good thing.
Not in a meaningful sense, no. Otherwise, Google Maps themselves would be AR (the locations are real - map markers are not).
We can talk about specifics, I linked one very particular job here (the one that I was fortunate enough to be allowed to write about, which is normally not the case) and I've invited you to give your own stories.

As you can see elsewhere in this thread I'm more than willing to change my tune and/or update the post if there is relevant information.

But your initial tone of voice + your categorical denial that these things are valuable makes it a bit harder to find common ground.

If you are scaling small poorly written systems then that already gives one very important data point that is divergent with the situation I've written about. The systems you start with are small, the systems I start with are usually large to very large and are running a mid to large enterprise and are - if you're lucky - a decade old or even much older. Either that or they are recent - and totally botched - rewrites.

If you are happy in your groove then more power to you but chances are that sooner or later you too will be handed a pile of manure without a shovel to go with it and maybe then you'll find some useful tips in that blogpost.

And the bit about the bookkeeping applies to your situation just as much as it does to larger and older systems.
When the "Hilton" hotel is actually a franchisee, aren't the aggregators and the "hotel chain" basically in the same business?

It's structured differently, but you still have a company with a (multi-)national brand presence funneling customers to independently owned hotels in exchange for a cut.

In a future where everyone books through hiltonhonors.com and spgdirect.com instead of booking.com, will the actual hotels make more money, or will the chains just start capturing more of that value for themselves?
Apple's new giant circle can move 4.5 feet! https://www.wired.com/2017/05/apple-park-new-silicon-valley-...
My guess it's because:

1. There are enough secretly addicted to opioids on HN

2. It's mostly (?) white people overdosing on opioids so it's finally waking up the majority

Either way hopefully this helps fix our collective disdain for addicts so we can kill the stigma and focus on treatment. Maybe there's room for a solid startup in that field.
Yes. (It's actually in 2 parts online.)
Sudo vulnerability
I can't wait for the server and laptop lines to come out. If nothing else, low-end server and laptop markets could do with a shake-up.
I think OP's point was it's replacing a lot of mental work vs the industrial revolution replacing physical work.

It can't do everything yet of course.
I would love to get 'put on the roof' like that, but I think the Japanese policy also states that you're not allowed to do anything outside of the ridiculously mundane task they assign you, and they actually watch you like a hawk to ensure as such. Pretty smart, most people aren't going to stay in that situation for long.
I was surprised to find that they are both owned by Mattel.
Goodbye PNaCl, Hello WebAssembly
Yeah but on Google Maps the markers represent a real place and in this game they represent a gym or Pokemon that is not.
with the exception of the log itself (and its in memory representation) it is a persistent data structure
Hawaii and the Ghost of Climate-Change Future
Going back to the previous page to https://serotiny.bio/api/parts/:id results in a page displaying the JSON response instead of the normal webpage.

( e.g. go to https://serotiny.bio/api/parts/11236, click 'edit in Pinecone', press cmd + [ ).
Bike-recovery app helps Vancouver reduce bike theft by 35% in past year
State of Origin 2017 live stream
Michele, I know you don't like me and I know you feel the need to insert your $0.02 wherever you feel you can stick the knife in. What drives you to do this I have absolutely no clue, but I'm sure you have your reasons. Enjoy.
This is a great concise list, however I would add one more item:

* Create bounties for individual features/bugfixes using a platform like Bountysource

I've contributed small amounts to FOSS projects I use personally, because I'd really like to see certain features added (or bugs fixed) but don't always have the means or time to contribute my own pull request.

Pros: customers only pay when the feature/bug is implemented, no pager duty or support hours, easy to gauge demand so time is only spent on work that's most desired by paying customers

Cons: one-time revenue, payments might be low relative to the work required

If I had an open source project with an active userbase (one day...), I would probably combine bounties with Patreon and/or support contracts.
> The delay point is much better. I don't think GameBoy style fight-to-capture would have been good, the technique used (basically Safari Zone, actually) was solid for that.

Only because the whole point of the game became grinding on simple pokemon to turn into candy to level up / evolve other Pokemon. It's a circle of crappy game mechanics requiring other crappy mechanics to work.

Sure, it would suck to need to actually fight 800 other magikarp to get a Gyarados, but that's not how any other pokemon game works. I can have my magikarp fight any other pokemon and gain xp.

I was able to do a red/blue style combat game for a homework assignment in Java. Not saying it's really easy, but it's not incredibly hard, and like you say, it's not harder than anti-cheat or many other things in the game. Also, if using a normal pokemon battle mechanic, you don't have all the issues with cheating at gym battles, and latency that the current game has.
All drives broken at the very least, a 10k rpm drive doesn't like ordinary vibrations already, much less 40cm+ displacement in a fraction of a second.

Heavy servers and especially UPS battery rows would likely take the whole rack down/toppling over with them. Plus: most cabling is secured to the walls/floors with not very much slack, which means a LOT of damaged/torn cables and FUBAR'd ports.

All in all, if an earthquake of the scale in the video would hit a typical European DC, it's bye-bye and rebuild.
Check out the videos and articles by Greg Young on the topic. This one's a good start https://www.youtube.com/watch?v=8JKjvY4etTY
I've considered marketing to email newsletters. What is your source for "email newsletters having a great ROI"?

It's been hard to come across meaningful data here.
It may be easy to engage the hotel directly, but when you are looking at potentially 20-30 hotels in an area the time the customer spends to check directly each one makes the aggregation a huge value proposition. I use booking.com a lot not because they are so good, but because they are more convenient than searching direct. I use to do motorcycle travels with a group and each day or couple of days we target the next place to go. With such a scenario booking hotels directly is impossible, but booking.com gives me everything in an area filtered by some criteria. I use location and rating as the top factors, price is just a differentiator. What is the hotel answer to that?
PS. Most of the time in my experience the booking.com price was better than direct booking. This is because on booking.com I found some great deals (not picking for, but why not use it?) while the hotels don't want to publish such low prices on their sites. I found out a nice place in Austria where we got by accident for a night, the place and the price were so good we extended the stay (for the same great price), then next year in the same period I checked the "regular" price and it was more than double.
Thats not really a good argument as if humans was intended to install it also here you could have machines to make sure it got properly connected just like we have for sillicon chips.

The argument actually speaks against what he was trying to say. He is basically saying that humans aren't clever enough to utilize optimal solutions which is a weakness not a strength.
Not if he gets a plea deal.
rant

I just finished cleaning my home folder out of the ~100,000 files npm created over the past couple of months. I just build interesting Node projects I come across to check them out and it's gotten that big. I wonder how it's like for regular node devs.
Not something we are currently looking at, but possible in the future.

I am not quite the right person to answer if the storage engine API can handle the use case for this. It is a slightly different problem, in that you need to push down a lot more conditions into the engine. In some ways we do this, with our MySQL cluster product already.
Perhaps, maybe his tune changes according to which side is paying him. Most of the quotes above are not reliant on his analysis though and the relevance of today's decision to Qualcomm's practices seems pretty clear.
Looks cool! Can you link to any experimental data/publications that show the functionality of enzymes that were designed this way?
It would be very interesting to get a feel for the success rate of such an approach.
You mention several research groups that use your tool, so I assume there is some in vitro/vivo data available.
Tools like NDEpend (for .NET) help me a bit with modularizing.

I doubt we'll ever see automation beyond what we do today in this space.
1) This was possible in node 7

2) A request can fail and can timeout.

3) Writing to a file can fail.

And you get the idea. Don't omit error handling. Error handling is business logic too.

Being relaxed around error handling is the recipe for cascading server failure.
Interesting. Do you have tons of side projects/other work you have available for potential employers to view? My sister only shows her degree and that she's been licensed in whichever state.

I'm in bioinformatics, so not pure tech, though I did interview for several straight tech-companies last year and my experience was very poor. Multiple phone-screens, having to work on side projects, on-site multi-hour interviews, white board exercises, etc.

My sister the travel nurse literally spends 2-5 hours on the phone every time she wants a new position. That generally includes 2-4 phone screens with potential employers. Then she gets offers and picks which job she wants. Are your interviews really that simple, it takes less than 5 hours to find a new job? You spend a few hours on the phone and then get immediate offers with relocation if needed?
I don't dislike you, I just dislike the degree to which you have zero respect for me. I think you are full well aware of the reasons I do the things I do.

I don't look to stick anyone with a knife. It is shocking how comfortable people are with casually watching my suffering and doing nothing about it while acting like any push back I give against the systemic issues that help keep me trapped in dire poverty somehow makes me evil incarnate.
>I think no one will ever dare to hire Levandowski. His career is over.

Yeah, things aren't looking awesome for him to work for anyone else. Perhaps he should look into starting his own company in the self driving car space. It worked out well last time he tried! /s
"Trivial"?

I think you meant to say "at least conceptually feasible".
You claimed human ingenuity was necessary yet here is an example of a solution that no human could have thought up or would have needed years to calculate yet it's better than any humans could have come up with.

I.e. computers are already used for ingenious solutions and that's really all that matters here.
States Where Single-Payer Health Care Could Work (If It Could Work Anywhere)
I gotta agree, even tho technically it is malware.
Is there really a lot of AI replacing highly trained professionals? There is a lot of hype around the idea, but I don't see any evidence of it happening in large scale. Using an AI tool will increase efficiency of course, but we've always had new developments that increase efficiency.

People undervalue how radically the word processor and spreadsheet changed how offices are run. But it didn't lead to a mass layoff of office workers.

I bet not even a single doctor has lost his job to AI yet.
Correct, a Value Added Tax. It may be necessary to levy additional taxes on high employment businesses hurt by automation (eg. Transportation). The responsible thing to do would be to tightly earmark the proceeds, and to have the taxes sunset, requiring a 2/3 majority to renew, after 10 years.
By returning the storage medium it is on?
Every async function will return a Promise anyway. I am simply interested in resolving a collection of Promises in a clean way.
Very impressive, actually.

More impressive are the guys walking around during all that swaying as if they were on coffee brake.
Ten Misconceptions About Software Testing
At this point, I really loathe adopting any facet of web-browser technology: there are too many broken APIs in too many browsers to maintain on both sides of the system: the browser developers have an insane number of combinations of features that need to be useful, secured and made reliable and developers for browsers are always at some weird disadvantage where they can spend months or years maintaining an application for the browser to find it rots out from underneath them.

Should you even be slightly successful in the use of an API, you always have to worry about deprecation when someone is no longer interested in doing the maintenance any more.

I am sure there were more than a few game developers that are are livid today about this announcement.

These things will go in cycles and I expect there will be an native-application cycle coming soon from browser-api-fatigue.
For context, the two Mythbusters presenters have famously different personalities and are not exactly friends. This reinforces the idea that pranks are generally done between people who feel they can trust one another.
> My thought was that the power supply should guarantee adequate power, or none at all. Not something in between. Also, no a rapidly alternating states of adequate power / no power.

That is very hard to do, actually. Voltage does fluctuate to a certain degree, because physics. And you don't want to cut power to a system just due to a small power dip. And you can't distinguish a small power dip from 10 small power dips over some time ago. And your flapping detection only works if the power drops are close enough to each other.

Proper control theory is amazingly hard. As my prof on that said - no one fully understands PID controllers, but some blokes have a really lucky thumb.
True. A "refill while you wait" service would be analogous, however.

And one could make an argument that, since printer cartridges are more fungible, the difference matters for cars but not for printer cartridges. IANAL, so I don't know if it makes any legal difference.
Self-esteem = productivity for many people. I'm pretty sure we do not want the population on a permanent vacation.
This is a really interesting point.

I don't know if this experiment has been done, but I actually think that there's a good chance that a bacterial cell might acquire a sequence that it itself contains - CRISPR is known to work at the population level but my understanding is that the mechanism for acquisition of new sequences is unclear.

I'll take a look for any papers on the topic and repost should I find anything.
Thats not what I mean. I mean that the competition is with whatever part of the brain the human uses for their job and that AI is taking on things that require higher and higher levels of abstraction.

For instance Radiologist. Ironically cleaning people are probably the last people who will be replaced as that require actual general intelligence AI
> I don't readily have a source at my fingertips, but I bet someone on HN can come up with a link.

That was us :) @patio11 was blown away by our in-browser Excel file preview: https://twitter.com/patio11/status/552765535239159808 (one of the replies suggested that he consider paying, and that led to our first contact) http://www.kalzumeus.com/2015/01/28/design-and-implementatio...
I'm very sorry about your friend. That is an absolute tragedy.
The end state is by no means clear.

You can extrapolate from recent results in (loosely) analogous domains if you want, but if so, it'd be just that -- extrapolation.
"We want the machines to take over but we all want to be sipping Mai Tais on the beach while it happens."

I don't think this will happen. Only the machine owners will sit at the beach and complain about the freeloaders they have to feed through welfare or UBI.

Otherwise we would already work 20 hour weeks.
You're thinking like an engineer. You probably also think that playing a video creates a copy just because it does. You probably also think Aereo's business model was legal just because it technically obeyed all of the laws.
On booking.com I take in consideration, in this order: user rating, location and features, pictures, price. The user rating is a hard filter (I don't even look below 8.0), the location is a given, the features and photos depend on the hotel, not the agregator, and are much more than a JSON. For example, no Wi-fi or paid Wi-fi in Europe is a deal killer, not a bonus, while a good food selection and hospitable staff is attractive.
>I totally dislike async, await, because they force you to switch to async code-flow-thinking.

If you started programming with Node, it's like you've learned doing everything the wrong way.

The async/await brings back the sane, synchronous, reasoning. There's a reason that from Lisp to Haskell, every language tried to get rid of the error-prone callback spaghetti.
I cleaned out a random code folder this weekend. Deleted nearly 2 million files from npm.