Search query: Cathodic photoelectrochemical bioanalysis

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abstract: Abstract Photoelectrochemical (PEC) bioanalysis is a newly developed exquisite methodology for innovative biomolecular detection. Photocathodes made by p type semiconductors have different photoelectric properties from that of n type semiconductors based photoanodes. Due to their obvious advantages and potentials, the integration of PEC bioanalysis and photocathodes has opened almost unlimited possibilities for elegant cathodic PEC bioanalysis. Currently, the impetus for advanced cathodic PEC bioanalysis has grown tremendously as demonstrated by increased research papers. Using illustrative examples, this review discusses specifically on this topic, with the aim to cover the basic concept, major signaling strategies, the state of the art in this field, and the future prospects based on our own opinions.
author_names: Yi-Tong Xu

Peng Qu
title: Cathodic photoelectrochemical bioanalysis
venue: TrAC Trends in Analytical Chemistry
year: 2019

abstract: A ZnAgInS quantum dot (QD) decorated BiOI microsphere (BiOI ZAIS) heterostructure was first proposed and successfully employed for sensitive cathodic photoelectrochemical (PEC) bioanalysis. Specifi.
author_names: Chun-Qin Zhao

Shou-Nian Ding

Jia-hui Xu

Hong-Yuan Chen
title: ZnAgInS Quantum Dot Decorated BiOI Heterostructure for Cathodic Photoelectrochemical Bioanalysis of Glucose Oxidase
year: 2020

abstract: Previously reported copper oxide based cathodic photoelectrochemical bioanalysis of cysteine had attributed the decrease of the photocurrents to the binding of cysteine onto the CuO surface. However, our latest investigation found that the previous explanation was not correct. This Letter presents the in depth study of such phenomena and a new insight into the underlying mechanism. Specifically, the unique redox reaction between the CuO photocathode and cysteine produced [Cys Cu(I) and cystine, and the insoluble complex blocked the partial contact between the photoelectrode and the dissolved O2 containing electrolyte and reduced the effective working area of the photocathode, leading to the decrease of photocurrent.
author_names: Yi-Li Liu

Yuancheng Zhu

Ling-bo Qu

Ran Yang

Xiao-Dong Yu

Wei-Wei Zhao
title: Unique Redox Reaction between CuO Photocathode and Cysteine: Insight into the Mechanism for Cathodic Photoelectrochemical Bioanalysis
venue: ACS Applied Bio Materials
year: 2019

abstract: This report outlines the first exploration of graphene oxide (GO) itself as a light harvesting material with an innovative in situ chemical redox and functionalization (CRF) strategy for versatile and high throughput cathodic photoelectrochemical (PEC) bioanalysis.
author_names: Tiantian Gu

Mengmeng Gu

Yi-Li Liu

Yuming Dong

Li-Bang Zhu

Zaijun Li

Guang-Li Wang

Wei-Wei Zhao
title: In situ chemical redox and functionalization of graphene oxide: toward new cathodic photoelectrochemical bioanalysis.
venue: Chemical communications
year: 2019

abstract: This work reports the liposome mediated in situ formation of the AgI/Ag/BiOI Z scheme heterojunction on foamed nickel electrode for signal on cathodic photoelectrochemical (PEC) bioanalysis. Specifically, in a proof of concept study, Ag nanoparticle encapsulated liposomes were initially confined via the sandwich immunobinding and then processed to release numerous Ag+ ions, which were then directed to react with the BiOI/Ni electrode, resulting in the in situ generation of a AgI/Ag/BiOI Z scheme heterojunction on the electrode. The enhanced cathodic signal could be correlated to the target concentration, which thus underlays a novel signal on cathodic liposomal PEC bioanalysis strategy. Different from previous anodic liposomal PEC bioanalysis, this work features the first cathodic liposomal PEC bioanalysis on the basis of the in situ formation of a Z scheme heterojunction. More generally, integrated with various biorecognition events, this protocol could serve as a common basis for addressing numerous targets of interest.
author_names: Si-Yuan Yu

Li-Ping Mei

Yi-Tong Xu

Tie-Ying Xue

Gaochao Fan

Deman Han

Guangxu Chen

Wei-Wei Zhao
title: Liposome Mediated in Situ Formation of AgI/Ag/BiOI Z Scheme Heterojunction on Foamed Nickel Electrode: A Proof of Concept Study for Cathodic Liposomal Photoelectrochemical Bioanalysis.
venue: Analytical chemistry
year: 2019

abstract: We report herein the energy transfer (ET) between semiconducting polymer dots (Pdots) and gold nanoparticles (Au NPs) in a photoelectrochemical (PEC) system and its feasibility for cathodic bioanalysis application. Specifically, COOH capped Pdots were first fabricated and then assembled onto the indium tin oxide (ITO) surface, followed by the modification of single strand (ss) DNA probe (pDNA) After the DNA hybridization with the Au NP tethered complementary ssDNA (Au NP tDNA) the Au NPs were brought into the close proximity of Pdots. Upon light stimulation, photoluminescence (PL) was annihilated, fluorescence was attenuated, and the photocurrent intensity was evidently decreased. This ET based PEC DNA sensor exhibited a linear range from 1 fM to 10 pM with a detection limit of 0.97 fM at a signal to noise ratio of 3. The present work first exploited the ET between Pdots and Au NPs, and we believe this phenomenon will spark new interest in the study of various Pdots based ET influenced PEC systems and thus catalyze increasing studies for specific bioanalytical purposes.
author_names: Xiao-Mei Shi

Li-Ping Mei

Qian Wang

Wei-Wei Zhao

Jing-Juan Xu

Hong-Yuan Chen
title: Energy Transfer between Semiconducting Polymer Dots and Gold Nanoparticles in a Photoelectrochemical System: A Case Application for Cathodic Bioanalysis.
venue: Analytical chemistry
year: 2018

abstract: Abstract In this study, we report the development of indirect Z scheme heterojunction to construct a cathodic photoelectrochemical (PEC) sensor for highly selective detection of endogenous H2S using AgCl Ag core shell nanostructures coupled with porous carbon nitride (PCN) as cathode materials. As an electron mediator, Ag on the Z scheme heterojunction transformed to Ag2S in the presence of H2S, resulting in the destruction of original charge carrier migration pathway. Meanwhile, photo induced charges on the then formed AgCl Ag2S/PCN cannot be as effectively separated as they are on AgCl Ag/PCN due to the straddling energy band alignments. A decrease in cathodic signal may be expected. Consequently, the H2S concentration could be quantitated by such corresponding decrease, which thus underlines a novel signal off cathodic PEC bioanalysis strategy. More importantly, the 'Z scheme' PEC sensor based on AgCl Ag/PCN exhibits analytical performance with high sensitivity, showing its potential to be applied to the areas of biomedical and health care etc.
author_names: Dong Liang

Junjun Luo

Yaliang Huang

X San Liang

Zaichun Zhou

Jianxiu Wang

Xiaoqing Qiu
title: Z Scheme cathodic photoelectrochemical sensors for detection of hydrogen sulfide based on AgCl Ag coupled with porous carbon nitride
year: 2020

abstract: Abstract Cathodic photoelectrochemical (PEC) bioanalysis has been drawing more attention in recent years due to the advantages of excellent anti interference capability. l cysteine (Cys) is an essential sulfur containing amino acid and has been intensively addressed as targets in previous anodic PEC bioanalysis. Herein a novel and simple cathodic PEC l cysteine (Cys) sensor is reported, on the basis of facile electrochemical fabrication of CuO/Cu2O heterojunction photocathode and the selective interaction between the copper and Cys via the formation of Cu S bond. The as prepared sensor exhibited excellent biosensing performance in terms of high sensitivity, selectivity and stability. In such a sensor, the binding induced decrease of cathodic photocurrent can be related to target concentration from 0 and 1.5 mM. This cathodic PEC Cys sensor exempted the use of expensive equipment and required no laborious sensor development procedures. We hope it can inspire more interest for further development of simple and practical PEC sensor toward important biomolecules.
author_names: Si-Yuan Yu

Yuan Gao

Fengzao Chen

Gaochao Fan

Deman Han

Chengshuang Wang

Wei-Wei Zhao
title: Fast electrochemical deposition of CuO/Cu2O heterojunction photoelectrode: Preparation and application for rapid cathodic photoelectrochemical detection of L cysteine
venue: Sensors and Actuators B: Chemical
year: 2019

abstract: In this study, a coumarin appended cyclometalated iridium (III) complex was prepared and demonstrated to be an efficient photoelectrochemical (PEC) active species with stable and reproducible cathodic photocurrent illuminated by visible light. A gold nanoparticles (AuNPs) based PEC probe was assembled using the as prepared iridium (III) complex as signal reporter. Integrating aptamer/protein proximity binding triggered strand displacement and catalytic hairpin assembly (CHA) amplification strategy, an enzyme free and sensitive PEC assay was developed. Benefiting from superior photon to current conversion character of the iridium (III) complex and effective amplification strategy, the proposed assay exhibited enhanced sensitivity for thrombin detection with a detection limit of 23 fM. It also showed a high specificity in serum samples. This study further demonstrated that cyclometalated iridium (III) complexes could be adopted as favorable photoactive material for bioanalysis by improving their ability of absorbance in the visible region.
author_names: Chunxiang Li

Yueyuan Cai

Mengmeng Pang

Xiaoming Zhou

Xiliang Luo

Zhenyu Xiao
title: A coumarin appended cyclometalated iridium(III) complex for visible light driven photoelectrochemical bioanalysis.
venue: Biosensors bioelectronics
year: 2019

abstract: Most of the photoelectrochemical (PEC) bioassays need to immobilize biomolecules on electrodes, which lead to tedious modification processes, damaged biomolecules, as well as crippled sensitivity/accuracy and low throughput of the performances. To overcome these drawbacks, we now introduce an exquisitely split mode (which separates the bioreaction (performed in microplates) from the PEC detection (conducted in PEC cell) cathodic photoelectrochemistry for probing versatile biocatalytic events with high throughput. Specifically, the enzymatically in situ generated 1,2 bezoquinone was covalently attached onto the PbSe quantum dots (QDs) modified indium tin oxide (ITO) (ITO/PbSe) photocathode through the connector of chitosan (CS) And the attached 1,2 bezoquinone acted as an efficient electron acceptor to promote the cathodic photocurrent of the ITO/PbSe electrode, enabling us to probe quinones generating oxidoreductase (by taking horseradish peroxidase (HRP) as a model) coupled biocatalytic cascades including the alkaline phosphatase (ALP)/HRP and the glucose oxidase (GOx)/HRP cascades. Quantitative probing for ALP activity in a wide linear range of 5.0 x 10 3 to 10 U/L with the detection limit of 1.2 x 10 3 U/L was realized. While a wide linear range of 5.0 x 10 8 to 1.0 x 10 4 moL/L with a quite low detection limit of 1.0 x 10 8 moL/L was obtained for the glucose assay. In addition, this testing protocol was also extended to an immunoassay (taking carcinoembryonic antigen (CEA) as an example) using HRP as a catalytic tracer. The developed bioassays show high sensitivity and good selectivity for CEA detection in the linear range from 0.1 pg/mL to 100 ng/mL with a detection limit of 0.02 pg/mL. Moreover, the proposed detection has distinctive merits because it not only avoids the adverse effects of the surface confined biomolecules for crippling the signal transduction, but also it has enhanced throughput.
author_names: Hong Wang

Fang Yuan

Xiuming Wu

Yuming Dong

Guang-Li Wang
title: Enzymatic in situ generation of covalently conjugated electron acceptor of PbSe quantum dots for high throughput and versatile photoelectrochemical bioanalysis.
venue: Analytica chimica acta
year: 2019