Mozart conducted the orchestra, Schikaneder himself played Papageno, while the role of the Queen of the Night was sung by Mozart's sister-in-law Josepha Hofer.
Mozart dirigoval orchester, Schikaneder sám stvárnil Papagena, zatiaľ čo v úlohe Kráľovnej noci účinkovala Mozartova švagriná Josepha Hoferová.