Margaret was very close to her paternal aunt, Marguerite de Navarre, who took care of her and her sister Madeleine during her childhood, and her sister-in-law Catherine de' Medici.
Marguerite sangat dekat dengan bibinya, Marguerite de Navarre, yang merawatnya dan saudarinya, Madeleine selama masa kecilnya, dan saudari iparnya Catherine de' Medici.