Mary was already unpopular, and her marriage on 15 May 1567 to James Hepburn, 4th Earl of Bothwell, who was widely suspected of murdering Darnley, heightened widespread bad feeling towards her.
Maria sudah tidak populer, dan pernikahannya pada tanggal 15 Mei 1567 dengan James Hepburn, Earl keempat Bothwell, yang secara luas dicurigai membunuh Darnley, meningkatkan perasaan buruk terhadapnya.