Iota and upsilon, which in Alexandrinus and many other manuscripts have two dots over them (diaeresis) when they commence a syllable – sometimes only one dot – have in the Codex Ephraemi a small straight line in their place.
Huruf-huruf iota dan upsilon, yang pada Alexandrinus dan banyak naskah lain ditambah dua titik di atasnya (tanda diaeresis) ketika mereka memulai suatu suku kata – kadang kala hanya satu titik – dalam Codex Ephraemi berupa satu garis pendek di atas.