Only after six years of marriage, Anna finally became pregnant, but died in childbirth; while some sources reported that her child, a son, died immediately after being baptized; others, said that he survived and was named Alessandro, dying in 1514 aged 17.
Baru setelah enam tahun menikah, Anna akhirnya hamil, namun meninggal saat melahirkan; sementara beberapa sumber melaporkan bahwa anaknya, seorang putra, meninggal segera setelah dibaptis; Yang lain, mengatakan bahwa ia selamat dan diberi nama Alessandro, meninggal pada tahun 1514 berusia 17 tahun.