As the player progresses through, Ezio can train new recruits to defend "dens" (Assassin HQs) and an upgraded Assassin's missions section called "Mediterranean Defense" in which the player works to strip control of various cities from Templar hands.
Saat pemain mengikuti alur permainan, Ezio dapat melatih rekrutan baru untuk melindungi "kandang" (Markas Assassin) dan bagian misi Assassin yang tingkatkan bermana "Mediterranean Defense" di mana pemain berusaha untuk merebut kontrol berbagai kota dari tangan pihak Templar.