Near 445 BC, her husband Megabyzus started a successful revolt in Syria against Artaxerxes I. Initially, Amytis stayed with the king during the war; however, she later participated, along with Amestris and the satrap Artarius, in the reconciliation negotiations between the rebel and the king.
Sekitar tahun 445 SM, suaminya Megabyzus memulai pemberontakan yang berhasil di Suriah melawan Artahsasta I. Awalnya, Amytis tinggal bersama raja selama perang; namun ia kemudian berpartisipasi bersama dengan Amestris dan Satrap Artarius, dalam negosiasi rekonsiliasi di antara pemberontak dan raja.