For the majority of time players would control an Assassin in the past history, while they also play as Desmond Miles or an Assassin Initiate in the present day, who hunt down their Templar targets.
Pemain akan mengontrol tokoh Assassin di masa lampau lebih sering, sementara mereka dapat pula bermain sebagai Desmond Miles atau Assassin Initiate di masa kini, yang memburu target-target Templar.