Following the death of his father, the family moved to Gothenburg where he went to school, and after graduation at the local "Realgymnasium" in 1921, he studied at the University of Uppsala, specializing in chemistry.
Menyusul kematian ayahnya, Tiselius sekeluarga pindah ke Göteborg di mana ia bersekolah, dan setelah lulus di "Realgymnasium" setempat pada tahun 1921, ia belajar di Universitas Uppsala, mengkhususkan diri dalam kimia.