In comparison to the relative and competitive quantification methods, comparative RT-PCR is considered to be the more convenient method to use since it does not require the investigator to perform a pilot experiment; in relative RT-PCR, the exponential amplification range of the mRNA must be predetermined and in competitive RT-PCR, a synthetic competitor RNA must be synthesized.
En comparación aos métodos de cuantificación competitivo e relativo, a RT-PCR comparativa considérase que é o método máis conveniente, xa que non se necesita que o investigador realice un experimento piloto; na RT-PCR relativa, o rango de amplificación exponencial do ARNm debe ser predeterminado e na RT-PCR competitiva, debe sintetizarse un ARN competidor sintético.