Enolase’s Lys345 deprotonates the alpha hydrogen, and the resulting negative charge is stabilized by resonance to the carboxylate oxygen and by the magnesium ion cofactors.
A Lys345 da enolase desprotona o hidróxeno alfa, e a carga negativa resultante é estabilizada por resonancia co oxíxeno do carboxilato e polos cofactores Mg2+.