However, the modified Michaelis-Menten equation assumes that binding of the inhibitor to the enzyme has reached equilibrium, which may be a very slow process for inhibitors with sub-nanomolar dissociation constants.
Porén, a ecuación de Michaelis-Menten modificada asume que a unión do inhibidor ao encima atingue o equilibrio, o cal pode ser un proceso moi lento para os inhibidores con constantes secundarias nanomolares de disociación.