After completion of the first dimension the complexes are destroyed by applying the denaturing SDS-PAGE in the second dimension, where the proteins of which the complexes are composed of are separated by their mass.
Despois de completar a primeira dimensión, os complexos son destruídos ao aplicar unha SDS-PAGE desnaturalizante na segunda dimensión, onde as proteínas de ditos complexos son separadas pola súa masa.