Unfavorable environmental conditions such as lack of nutrients or oxygen, extreme temperatures, lack of moisture and presence of toxic chemicals, which are not conducive for the growth of the microbe trigger the formation of a cyst.
As condicións ambientais desfavorables como a falta de nutrientes ou oxíxeno, as temperaturas extremas, a falta de humidade e a presenza de compostos químicos tóxicos, que non contribúen ao crecemento do microbio desencadean a formación do quiste.