While the majority of the His320 ligands and bound water will not be in their active forms (protonated and deprotonated, respectively,) it was calculated that approximately 0.3% of total urease enzyme would be active at any one time.
Aínda que a maioría dos ligandos His320 e a auga unida non están nas súas formas activas (protonada e desprotonada, respectivamente), calculouse que aproximadamente o 0,3% do encima urease total estaría sempre activo.