It was the discovery of enzymes at the beginning of the 20th century by Eduard Buchner that separated the study of the chemical reactions of metabolism from the biological study of cells, and marked the beginnings of biochemistry.
O descubrimento dos encimas por Eduard Buchner a principios do século XX permitiu separar o estudo das reaccións químicas metabólicas do estudo biolóxico das células, e significou o comezo da bioquímica.