When co-transfected with T7 RNA polymerase (T7 RNAP) into cultured human osteosarcoma 143B cells, about 10-20% of the cells were found to express HSV-TK, and more than 90% of the cells were killed in the presence of 1 μM ganciclovir (GCV) within 4 days after DNA transfection."
Cando é cotransfectado coa ARN polimerase de T7 (T7 RNAP) en células de osteosarcoma humano 143B cultivadas, observouse que un 10-20% das células expresaban a HSV-TK e máis do 90% das células morrían en presenza de ganciclovir (GCV) 1 μM nos 4 días posteriores á transfección."