rudyvdbrink/CitationDatabase huggingface dataset:
Observational methods used to assess rat behavior: general principles.
CSH protocols, 2007

INTRODUCTIONThe behavioral approach to solving neuroscience questions, unlike cellular and molecular approaches, is difficult to define and is therefore sometimes considered a less-disciplined approach. Methods describing behavioral procedures are often thought to be crude when compared to the precision of physiological or molecular methods. This apparent lack of precision results from the problems inherent in the study of behavior--deciding what to measure among the large number of variables, understanding that behavior is the final output of a vast array of neurochemical and electrophysiological changes, designing reproducible experiments that have appropriate controls, and most importantly, interpreting results. Conflicting results from similar protocols have led to prolonged debates and controversies in interpretation of behavioral data; therefore, it is necessary to strive for a high degree of precision through the cultivation of excellent handling and observational skills and thorough documentation strategies. This article is an introduction to several methods used to measure and assess normal behavior in a rat, including suggestions about how to make objective behavioral observations. Behavioral tests can be used to establish a "behavioral baseline" for a normal animal, to assess the effects of drugs or a lesion on behavior, and to determine whether transgenic animals have a normal behavioral repertoire. It should be kept in mind that, along with the intended effect, experimental manipulations often have unintended side effects, and behavioral tests can also be used to determine some of the less obvious changes.
Tetrahydrofuran-Based Transient Receptor Potential Ankyrin 1 (TRPA1) Antagonists: Ligand-Based Discovery, Activity in a Rodent Asthma Model, and Mechanism-of-Action via Cryogenic Electron Microscopy.
Journal of medicinal chemistry, 2021

Transient receptor potential ankyrin 1 (TRPA1) is a nonselective calcium-permeable ion channel highly expressed in the primary sensory neurons functioning as a polymodal sensor for exogenous and endogenous stimuli and has generated widespread interest as a target for inhibition due to its implication in neuropathic pain and respiratory disease. Herein, we describe the optimization of a series of potent, selective, and orally bioavailable TRPA1 small molecule antagonists, leading to the discovery of a novel tetrahydrofuran-based linker. Given the balance of physicochemical properties and strong
Adolescent Neurodevelopment and Vulnerability to Psychosis.
Biological psychiatry, 2021

Adolescence is characterized by significant changes in several domains, including brain structure and function, puberty, and social and environmental factors. Some of these changes serve to increase the likelihood of psychosis onset during this period, while others may buffer this risk. This review characterizes our current knowledge regarding the unique aspects of adolescence that may serve as risk factors for schizophrenia spectrum disorders. In addition, we provide potential future directions for research into adolescent-specific developmental mechanisms that impart vulnerability to psychosis and the possibility of interventions that capitalize on adolescents' unique characteristics. Specifically, we explore the ways in which gray and white matter develop throughout adolescence in typically developing youth as well as in those with psychosis spectrum disorders. We also discuss current views on the function that social support and demands, as well as role expectations, play in risk for psychosis. We further highlight the importance of considering biological factors such as puberty and hormonal changes as areas of unique vulnerability for adolescents. Finally, we discuss cannabis use as a factor that may have a unique impact during adolescent neurodevelopment, and subsequently potentially impact psychosis onset. Throughout, we include discussion of resilience factors that may provide unique opportunities for intervention during this dynamic life stage.
Neuroscience Symposium and Workshop on SAAPCON-2016, Nepal.
Annals of neurosciences, 2017

Physiological Society of Nepal, with support from the South Asian Association of Physiologists (SAAP), organized the 5th Biennial Conference of the South Asian Association of Physiologists in conjunction with the 2nd Annual Conference of Physiological Society of Nepal. The purpose of this conference is to bring the scientists, researchers, and students from the South Asian countries in one platform to share and discuss the recent advances and achievements in the field of physiology. Since physiology is the backbone of medical science which deals with the functions of the human body, the theme of the conference was given as "Redefining health in nature." Giving the emphasis on this theme, the conference was organized on November 10-14, 2016, in Kathmandu University, Dhulikhel, Nepal. A total of 300 participants from 15 countries including the South Asian Association of Regional Cooperation (SAARC) and Non-SAARC countries participated in the conference. The scientific program of main conference, which ran for 2 days, was divided into 7 symposia - neuroscience, cardiopulmonary physiology, cellular and integrative physiology, health and lifestyle, role of physiology and medical education, endocrine and reproductive physiology, and high altitude and respiratory physiology - to highlight the latest progresses made in the field of physiology around the globe. Neuroscience symposium held on the second day consisted of 7 advance lectures and 3 young scientist presentations and poster session. Post-conference workshop on system neuroscience was held in BP Koirala Institute of Health Sciences, Dharan, Nepal.
Proposal of a new method for measuring Förster Resonance Energy Transfer (FRET) rapidly, quantitatively and non-destructively.
International journal of molecular sciences, 2012

The process of radiationless energy transfer from a chromophore in an excited electronic state (the "donor") to another chromophore (an "acceptor"), in which the energy released by the donor effects an electronic transition, is known as "Förster Resonance Energy Transfer" (FRET). The rate of energy transfer is dependent on the sixth power of the distance between donor and acceptor. Determining FRET efficiencies is tantamount to measuring distances between molecules. A new method is proposed for determining FRET efficiencies rapidly, quantitatively, and non-destructively on ensembles containing donor acceptor pairs: at wavelengths suitable for mutually exclusive excitations of donors and acceptors, two laser beams are intensity-modulated in rectangular patterns at duty cycle ½ and frequencies ƒ(1) and ƒ(2) by electro-optic modulators. In an ensemble exposed to these laser beams, the donor excitation is modulated at ƒ(1), and the acceptor excitation, and therefore the degree of saturation of the excited electronic state of the acceptors, is modulated at ƒ(2). Since the ensemble contains donor acceptor pairs engaged in FRET, the released donor fluorescence is modulated not only at ƒ(1) but also at the beat frequency Δƒ: = |ƒ(1) - ƒ(2)|. The depth of the latter modulation, detectable via a lock-in amplifier, quantitatively indicates the FRET efficiency.
Assessment of cognitive and neural recovery in survivors of pediatric brain tumors in a pilot clinical trial using metformin.
Nature medicine, 2020

We asked whether pharmacological stimulation of endogenous neural precursor cells (NPCs) may promote cognitive recovery and brain repair, focusing on the drug metformin, in parallel rodent and human studies of radiation injury. In the rodent cranial radiation model, we found that metformin enhanced the recovery of NPCs in the dentate gyrus, with sex-dependent effects on neurogenesis and cognition. A pilot double-blind, placebo-controlled crossover trial was conducted (ClinicalTrials.gov, NCT02040376) in survivors of pediatric brain tumors who had been treated with cranial radiation. Safety, feasibility, cognitive tests and MRI measures of white matter and the hippocampus were evaluated as endpoints. Twenty-four participants consented and were randomly assigned to complete 12-week cycles of metformin (A) and placebo (B) in either an AB or BA sequence with a 10-week washout period at crossover. Blood draws were conducted to monitor safety. Feasibility was assessed as recruitment rate, medication adherence and procedural adherence. Linear mixed modeling was used to examine cognitive and MRI outcomes as a function of cycle, sequence and treatment. We found no clinically relevant safety concerns and no serious adverse events associated with metformin. Sequence effects were observed for all cognitive outcomes in our linear mixed models. For the subset of participants with complete data in cycle 1, metformin was associated with better performance than placebo on tests of declarative and working memory. We present evidence that a clinical trial examining the effects of metformin on cognition and brain structure is feasible in long-term survivors of pediatric brain tumors and that metformin is safe to use and tolerable in this population. This pilot trial was not intended to test the efficacy of metformin for cognitive recovery and brain growth, but the preliminary results are encouraging and warrant further investigation in a large multicenter phase 3 trial.
Differential attenuation of β2 integrin-dependent and -independent neutrophil migration by Ly6G ligation.
Blood advances, 2019

Antibody ligation of the murine neutrophil surface protein Ly6G disrupts neutrophil migration in some contexts but not others. We tested whether this variability reflected divergent dependence of neutrophil migration on β2 integrins, adhesion molecules that interact with Ly6G at the neutrophil surface. In integrin-dependent murine arthritis, Ly6G ligation attenuated joint inflammation, even though mice lacking Ly6G altogether developed arthritis normally. By contrast, Ly6G ligation had no impact on integrin-independent neutrophil migration into inflamed lung. In peritoneum, the role of β2 integrins varied with stimulus, proving dispensable for neutrophil entry in
Early detection of probable idiopathic Parkinson's disease: I. development of a diagnostic test battery.
Movement disorders : official journal of the Movement Disorder Society, 2000

We developed a test battery as an inexpensive and objective aid for the early diagnosis of idiopathic Parkinson's disease (iPD) and its differential diagnoses. The test battery incorporates tests of motor function, olfaction, and mood. In the motor task, a wrist flexion-and-extension task to different targets, movement velocities were recorded. Olfaction was tested with the University of Pennsylvania Smell Identification Test. Mood was assessed with the Beck Depression Inventory. An initial regression model was developed from the results of 19 normal control subjects and 18 patients with early, mild, probable iPD. Prospective application to an independent validation set of 122 normal control subjects and 103 patients resulted in an 88% specificity rate and 69% sensitivity rate, with an area under the Receiver Operator Characteristic curve of 0.87.
Microstructure of Human Corpus Callosum across the Lifespan: Regional Variations in Axon Caliber, Density, and Myelin Content.
Cerebral cortex (New York, N.Y. : 1991), 2021

The myeloarchitecture of the corpus callosum (CC) is characterized as a mosaic of distinct differences in fiber density of small- and large-diameter axons along the anterior-posterior axis; however, regional and age differences across the lifespan are not fully understood. Using multiecho T2 magnetic resonance imaging combined with multi-T2 fitting, the myelin water fraction (MWF) and geometric-mean of the intra-/extracellular water T2 (geomT2IEW) in 395 individuals (7-85 years; 41% males) were examined. The approach was validated where regional patterns along the CC closely resembled the histology; MWF matched mean axon diameter and geomT2IEW mirrored the density of large-caliber axons. Across the lifespan, MWF exhibited a quadratic association with age in all 10 CC regions with evidence of a positive linear MWF-age relationship among younger participants and minimal age differences in the remainder of the lifespan. Regarding geomT2IEW, a significant linear age × region interaction reflected positive linear age dependence mostly prominent in the regions with the highest density of small-caliber fibers-genu and splenium. In all, these two indicators characterize distinct attributes that are consistent with histology, which is a first. In addition, these results conform to rapid developmental progression of CC myelination leveling in middle age as well as age-related degradation of axon sheaths in older adults.
Alcohol and Cocaine Combined Substance Use on Adult Hypothalamic Neural Stem Cells and Neurogenesis.
Brain plasticity (Amsterdam, Netherlands), 2020

Many advancements have been made over the years looking at the individual and combined effects of drugs of abuse on the brain, with one key area of research focusing on the effects on neurogenesis. An integral part of fetal brain development and, later, maintenance in the adult brain, neurogenesis occurs in three main regions: subventricularzone of the lateral ventricles (SVZ), subgranularzone of the dentate gyrus (SGZ), and the tanycyte layer in the hypothalamus (TL). We will review current literature on combined drugs of abuse and their effect on adult neurogenesis. More specifically, this review will focus on the effect of combining cocaine and alcohol. Additionally, the tanycyte layer will be explored in more depth and probed to look at the neurogenic properties of tanycytes and their role in neurogenesis.
The 'Scientific' phrenologist - Bernard Hollander (1864-1934).
Journal of medical biography, 2021

Bernard Hollander (1864-1934), a Viennese-born British physician, scientist, and author, was best known for his late 19th century and early 20th century revival of a 'Scientific Phrenology'. Hollander, motivated by the advances in cerebral localisation and neuroscience that appeared to justify Franz Joseph Gall's (1758-1828) initial interests in craniology, hoped to use this new framework to substantively improve the lot of his patients and his community. Ridiculed and derided by his colleagues while maintaining a measure of public prominence, Hollander discussed contemporary issues including notions of human nature, mental illness, education, development, women's rights, and sociobiology. The current work focuses on Hollander, his writings, and his reception by the contemporary medical and lay community.
Extrafoveally applied flashing light affects contrast thresholds of achromatic and S-cone isolating, but not L-M cone modulated stimuli.
Neuroscience letters, 2018

Flashing light stimulation is often used to investigate the visual system. However, the magnitude of the effect of this stimulus on the various subcortical pathways is not well investigated. The signals of conscious vision are conveyed by the magnocellular, parvocellular and koniocellular pathways. Parvocellular and koniocellular pathways (or more precisely, the L-M opponent and S-cone isolating channels) can be accessed by isoluminant red-green (L-M) and S-cone isolating stimuli, respectively. The main goal of the present study was to explore how costimulation with strong white extrafoveal light flashes alters the perception of stimuli specific to these pathways. Eleven healthy volunteers with negative neurological and ophthalmological history were enrolled for the study. Isoluminance of L-M and S-cone isolating sine-wave gratings was set individually, using the minimum motion procedure. The contrast thresholds for these stimuli as well as for achromatic gratings were determined by an adaptive staircase procedure where subjects had to indicate the orientation (horizontal, oblique or vertical) of the gratings. Thresholds were then determined again while a strong white peripheral light flash was presented 50 ms before each trial. Peripheral light flashes significantly (p < 0.05) increased the contrast thresholds of the achromatic and S-cone isolating stimuli. The threshold elevation was especially marked in case of the achromatic stimuli. However, the contrast threshold for the L-M stimuli was not significantly influenced by the light flashes. We conclude that extrafoveally applied light flashes influence predominantly the perception of achromatic stimuli.
Exosomal MicroRNAs in Military Personnel with Mild Traumatic Brain Injury: Preliminary Results from the Chronic Effects of Neurotrauma Consortium Biomarker Discovery Project.
Journal of neurotrauma, 2020

Chronic symptoms after mild traumatic brain injury (mTBI) are common among veterans and service members, and represent a significant source of morbidity, with those who sustain multiple mTBIs at greatest risk. Exosomal micro-RNAs (miRNAs), mediators of intercellular communication, may be involved in chronic TBI symptom persistence. Exosomal miRNA (exomiR) was extracted from 153 participants enrolled in the Chronic Effect of Neurotrauma Consortium (CENC) longitudinal study (no TBI,
Intraoperative Neurophysiology for Optimization of Percutaneous Spinothalamic Cordotomy for Intractable Cancer Pain.
Operative neurosurgery (Hagerstown, Md.), 2020

Percutaneous ablation of the cervical spinothalamic tract (STT) remains a therapeutic remedy for intractable cancer pain. However, it is accompanied by the risk of collateral damage to essential spinal cord circuitry, including the corticospinal tract (CST). Recent studies describe threshold-based mapping of the CST with the objective of motor bundle preservation during intramedullary spinal cord and supratentorial surgery.
Important Difference between Occupational Hazard Exposure among Shift Workers and Other Workers; Comparing Workplace before and after 1980.
International journal of environmental research and public health, 2020

Improving health and safety at work has been an important issue for the European Union since the 1980s. The existing literature supports that shift work is associated with multiple indicators of poor health but frequently neglects the potential impact of occupational hazards. This study aims at describing and comparing the exposure to different workplace hazards among shift and other workers before and after 1980. Exposure to different workplace hazards (noise, dust, pollutant, and other physical stressors) were analyzed among 119,413 participants from the UK Biobank cohort. After stratifying the analyses before and after 1980, exposure was compared between shift and other workers. Potential confounding variables (sex, age, ethnicity, education level, occupational category, and neuroticism) were adjusted for in the log-binomial regression. Shift workers had a higher prevalence ratio (PR) than other workers of being exposed to almost all identified hazards both before or after 1980. They were also more likely to be exposed to multiple hazards compared to other workers, both before 1980 (PR: 1.25; 95% CI: 1.21-1.30) and after 1980 (PR: 1.34; 95% CI: 1.30-1.38). The prevalence of all measured risk factors was higher after 1980 than before 1980 among shift workers. Of note, the work environment has improved overall for other workers. Our findings suggest that changes at the workplace have benefited other workers more than shift workers as they are still more exposed to all occupational hazards.
Biomedical Signal Acquisition Using Sensors under the Paradigm of Parallel Computing.
Sensors (Basel, Switzerland), 2020

There are several pathologies attacking the central nervous system and diverse therapies for each specific disease. These therapies seek as far as possible to minimize or offset the consequences caused by these types of pathologies and disorders in the patient. Therefore, comprehensive neurological care has been performed by neurorehabilitation therapies, to improve the patients' life quality and facilitating their performance in society. One way to know how the neurorehabilitation therapies contribute to help patients is by measuring changes in their brain activity by means of electroencephalograms (EEG). EEG data-processing applications have been used in neuroscience research to be highly computing- and data-intensive. Our proposal is an integrated system of Electroencephalographic, Electrocardiographic, Bioacoustic, and Digital Image Acquisition Analysis to provide neuroscience experts with tools to estimate the efficiency of a great variety of therapies. The three main axes of this proposal are: parallel or distributed capture, filtering and adaptation of biomedical signals, and synchronization in real epochs of sampling. Thus, the present proposal underlies a general system, whose main objective is to be a wireless benchmark in the field. In this way, this proposal could acquire and give some analysis tools for biomedical signals used for measuring brain interactions when it is stimulated by an external system during therapies, for example. Therefore, this system supports extreme environmental conditions, when necessary, which broadens the spectrum of its applications. In addition, in this proposal sensors could be added or eliminated depending on the needs of the research, generating a wide range of configuration limited by the number of CPU cores, i.e., the more biosensors, the more CPU cores will be required. To validate the proposed integrated system, it is used in a Dolphin-Assisted Therapy in patients with Infantile Cerebral Palsy and Obsessive-Compulsive Disorder, as well as with a neurotypical one. Event synchronization of sample periods helped isolate the same therapy stimulus and allowed it to be analyzed by tools such as the Power Spectrum or the Fractal Geometry.
Drug Conditioning and Sensitization in Mice: Importance of Early Voluntary Exercise.
Neuroscience, 2020

The timing of voluntary exercise relative to drug conditioning is important to its "neuroprotective" effects, though it is unclear whether the voluntary exercise needs to occur temporally contiguous with drug conditioning, or occur during an early, developmental period, but non-contiguous with drug conditioning, for its "neuroprotective" effects. To distinguish between these two ideas, the timing of voluntary exercise relative to drug conditioning on the development and extinction of conditioned hyperactivity, and induction of sensitization was manipulated in the present experiment. Specifically, half of the exercise mice were permitted access to home-cage running wheels for 6 continuous weeks (Exercise-Exercise) whereas the other half of the exercise mice were permitted access to home-cage running wheels only for the first 3  weeks and then had the wheels removed (Exercise-Sedentary). Likewise, half of the sedentary mice had no home-cage running wheels for 6 continuous weeks (Sedentary-Sedentary) whereas the other half of the sedentary mice were permitted access to the home-cage running wheels for the last 3 weeks prior to the acquisition phase (Sedentary-Exercise). Mice received vehicle or methamphetamine (Meth; 1.0 mg/kg; acquisition), followed by saline-alone sessions (extinction) and finally challenged with an escalating Meth-regimen (0.25 → 1.0 mg/kg). While all Meth-paired groups, regardless of exercise regimen, showed conditioned hyperactivity, Exercise-Exercise and Exercise-Sedentary mice were less responsive to chronic Meth exposure and showed slower extinction compared to the other Meth-paired groups. These results suggest an early exercise regimen, during a critical developmental window, protects against the stimulant properties of Meth and simultaneously facilitates contextual learning.