rudyvdbrink/CitationDatabase huggingface dataset:
Functional dissociation of temporal processing mechanisms during speech production and hand movement: An ERP study.
Behavioural brain research, 2018

Skilled motor actions are mediated by neural mechanisms that incorporate sensory feedback for driving or suppressing movement with remarkable temporal precision. The predictive coding model proposes that the brain performs this function by establishing an internal representation of timing to accelerate movement response time. However, it is unclear whether different neural mechanisms are involved in temporal processing of movement initiation and cessation. The present study examined how temporal information is encoded for initiation and cessation of speech and hand movement. Event-related potentials (ERPs) were recorded while young healthy subjects performed speech and hand movement initiation and cessation in response to temporally predictable and unpredictable visual stimuli. We found that predictable stimuli elicited faster movement in both speech and hand modalities, with shorter reaction times associated with movement cessation compared with initiation. Analysis of ERPs revealed that premotor neural activities were significantly attenuated before speech initiation and hand movement initiation and cessation for temporally predictable vs. unpredictable conditions, but an opposite pattern was observed for speech cessation. In addition, we observed that the premotor ERPs were significantly modulated during speech initiation vs. cessation, but no such effect was found during hand movement. Finally, we found that the premotor ERPs were strongly correlated with motor reaction time during movement initiation and cessation for speech and hand modalities only in response to temporally predictable stimuli. These findings indicate that premotor ERPs reflect a temporal predictive code for planning of movement initiation and cessation and highlight functional dissociation of temporal processing mechanisms in speech and hand motor systems.
Confirmatory Factor Analysis of the Dutch Version of the Wechsler Memory Scale-Fourth Edition (WMS-IV-NL).
Archives of clinical neuropsychology : the official journal of the National Academy of Neuropsychologists, 2015

The latent factor structure of the Dutch version of the Wechsler Memory Scale-Fourth Edition (WMS-IV-NL) was examined with a series of confirmatory factor analyses. As part of the Dutch standardization, 1,188 healthy participants completed the WMS-IV-NL. Four models were tested for the Adult Battery (16-69 years; N = 699), and two models were tested for the Older Adult Battery (65-90 years; N = 489). Results corroborated the presence of three WMS-IV-NL factors in the Adult Battery consisting of Auditory Memory, Visual Memory, and Visual Working Memory. A two-factor model (consisting of Auditory Memory and Visual Memory) provided the best fit for the data of the Older Adult Battery. These findings provide evidence for the structural validity of the WMS-IV-NL, and further support the psychometric integrity of the WMS-IV.
Fluorescence Imaging Methods to Investigate Translation in Single Cells.
Cold Spring Harbor perspectives in biology, 2019

Translation is the fundamental biological process that converts the genetic information in messenger RNAs (mRNAs) into functional proteins. Translation regulation allows cells to control when, where, and how many proteins are synthesized. Much of what we know about translation comes from ensemble approaches that measure the average of many cells. The cellular and molecular heterogeneity in the regulation of translation remains largely elusive. Fluorescence microscopy allows interrogation of biological problems with single-molecule, single-cell sensitivity. In recent years, improved design of reagents and microscopy tools has led to improved spatial and temporal resolution of translation imaging. It is now possible to track global translation in specific subcellular compartments and follow the translation dynamics of single transcripts. Highlighted here is the recent progress in translation imaging with emphasis on in vivo translation dynamics. These tools will be invaluable to the study of translation regulation.
Small noncoding RNA profiling across cellular and biofluid compartments and their implications for multiple sclerosis immunopathology.
Proceedings of the National Academy of Sciences of the United States of America, 2021

Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease affecting the central nervous system (CNS). Small non-coding RNAs (sncRNAs) and, in particular, microRNAs (miRNAs) have frequently been associated with MS. Here, we performed a comprehensive analysis of all classes of sncRNAs in matching samples of peripheral blood mononuclear cells (PBMCs), plasma, cerebrospinal fluid (CSF) cells, and cell-free CSF from relapsing-remitting (RRMS,
C. elegans Notch signaling regulates adult chemosensory response and larval molting quiescence.
Current biology : CB, 2011

The conserved DOS-motif proteins OSM-7 and OSM-11 function as coligands with canonical DSL (Delta, Serrate, and LAG-2) ligands to activate C. elegans Notch receptors during development. We report here that Notch ligands, coligands, and the receptors LIN-12 and GLP-1 regulate two C. elegans behaviors: chemosensory avoidance of octanol and quiescence during molting lethargus.
Synapsins contribute to the dynamic spatial organization of synaptic vesicles in an activity-dependent manner.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012

The precise subcellular organization of synaptic vesicles (SVs) at presynaptic sites allows for rapid and spatially restricted exocytotic release of neurotransmitter. The synapsins (Syns) are a family of presynaptic proteins that control the availability of SVs for exocytosis by reversibly tethering them to each other and to the actin cytoskeleton in a phosphorylation-dependent manner. Syn ablation leads to reduction in the density of SV proteins in nerve terminals and increased synaptic fatigue under high-frequency stimulation, accompanied by the development of an epileptic phenotype. We analyzed cultured neurons from wild-type and Syn I,II,III(-/-) triple knock-out (TKO) mice and found that SVs were severely dispersed in the absence of Syns. Vesicle dispersion did not affect the readily releasable pool of SVs, whereas the total number of SVs was considerably reduced at synapses of TKO mice. Interestingly, dispersion apparently involved exocytosis-competent SVs as well; it was not affected by stimulation but was reversed by chronic neuronal activity blockade. Altogether, these findings indicate that Syns are essential to maintain the dynamic structural organization of synapses and the size of the reserve pool of SVs during intense SV recycling, whereas an additional Syn-independent mechanism, whose molecular substrate remains to be clarified, targets SVs to synaptic boutons at rest and might be outpaced by activity.
Arousal effect of caffeine depends on adenosine A2A receptors in the shell of the nucleus accumbens.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011

Caffeine, the most widely used psychoactive compound, is an adenosine receptor antagonist. It promotes wakefulness by blocking adenosine A(2A) receptors (A(2A)Rs) in the brain, but the specific neurons on which caffeine acts to produce arousal have not been identified. Using selective gene deletion strategies based on the Cre/loxP technology in mice and focal RNA interference to silence the expression of A(2A)Rs in rats by local infection with adeno-associated virus carrying short-hairpin RNA, we report that the A(2A)Rs in the shell region of the nucleus accumbens (NAc) are responsible for the effect of caffeine on wakefulness. Caffeine-induced arousal was not affected in rats when A(2A)Rs were focally removed from the NAc core or other A(2A)R-positive areas of the basal ganglia. Our observations suggest that caffeine promotes arousal by activating pathways that traditionally have been associated with motivational and motor responses in the brain.
Fxr1 regulates sleep and synaptic homeostasis.
The EMBO journal, 2020

The fragile X autosomal homolog 1 (Fxr1) is regulated by lithium and has been GWAS-associated with schizophrenia and insomnia. Homeostatic regulation of synaptic strength is essential for the maintenance of brain functions and involves both cell-autonomous and system-level processes such as sleep. We examined the contribution of Fxr1 to cell-autonomous homeostatic synaptic scaling and neuronal responses to sleep loss, using a combination of gene overexpression and Crispr/Cas9-mediated somatic knockouts to modulate gene expression. Our findings indicate that Fxr1 is downregulated during both scaling and sleep deprivation via a glycogen synthase kinase 3 beta (GSK3β)-dependent mechanism. In both conditions, downregulation of Fxr1 is essential for the homeostatic modulation of surface AMPA receptors and synaptic strength. Preventing the downregulation of Fxr1 during sleep deprivation results in altered EEG signatures. Furthermore, sequencing of neuronal translatomes revealed the contribution of Fxr1 to changes induced by sleep deprivation. These findings uncover a role of Fxr1 as a shared signaling hub between cell-autonomous homeostatic plasticity and system-level responses to sleep loss, with potential implications for neuropsychiatric illnesses and treatments.
Functional fabrication of recombinant human collagen-phosphorylcholine hydrogels for regenerative medicine applications.
Acta biomaterialia, 2015

The implant-host interface is a critical element in guiding tissue or organ regeneration. We previously developed hydrogels comprising interpenetrating networks of recombinant human collagen type III and 2-methacryloyloxyethyl phosphorylcholine (RHCIII-MPC) as substitutes for the corneal extracellular matrix that promote endogenous regeneration of corneal tissue. To render them functional for clinical application, we have now optimized their composition and thereby enhanced their mechanical properties. We have demonstrated that such optimized RHCIII-MPC hydrogels are suitable for precision femtosecond laser cutting to produce complementing implants and host surgical beds for subsequent tissue welding. This avoids the tissue damage and inflammation associated with manual surgical techniques, thereby leading to more efficient healing. Although we previously demonstrated in clinical testing that RHCIII-based implants stimulated cornea regeneration in patients, the rate of epithelial cell coverage of the implants needs improvement, e.g. modification of the implant surface. We now show that our 500μm thick RHCIII-MPC constructs comprising over 85% water are suitable for microcontact printing with fibronectin. The resulting fibronectin micropatterns promote cell adhesion, unlike the bare RHCIII-MPC hydrogel. Interestingly, a pattern of 30μm wide fibronectin stripes enhanced cell attachment and showed the highest mitotic rates, an effect that potentially can be utilized for faster integration of the implant. We have therefore shown that laboratory-produced mimics of naturally occurring collagen and phospholipids can be fabricated into robust hydrogels that can be laser profiled and patterned to enhance their potential function as artificial substitutes of donor human corneas.
Bony Hyperostosis Recurrence after Complete Resection of Sphenoorbital Meningioma.
Cureus, 2017

Bony hyperostosis is commonly associated with meningioma growth and is considered one of the characteristic signs on imaging; however, recurrence of meningiomas in the sphenoorbital area, including associated hyperostosis, is typically precluded by gross total resection of the lesion. This 63-year-old man presented with progressive double vision and proptosis in the right eye. He underwent frontotemporal craniotomy and partial removal after magnetic resonance imaging demonstrated a right sphenoorbital meningioma extending to the orbit and middle fossa. He had transient improvement of his symptoms postoperatively but experienced a progressive recurrence of symptoms and new onset of right facial hypoesthesia in the distribution of V1 and V2. We performed a right frontotemporal craniotomy with removal of the nodular part, as well as extensive drilling. Although the postoperative computed tomography scan revealed a gross total resection, the five-year follow-up scan demonstrated a recurrent hyperostosis in the region of the lesser and greater sphenoid wings, the middle cranial fossa floor with inferior extension toward the infratemporal fossa, and the sphenoid sinus wall. After another redo surgery, the patient continues to be monitored with yearly imaging. The extent of surgical resection is one of the most important predictors of meningioma recurrence postoperatively, and cases of recurrence after gross total resection are rare.
Cognitive control and midline theta adjust across multiple timescales.
Neuropsychologia, 2018

Cognitive control of attention in conflict situations is a basic skill that is vital for goal-oriented behavior. Behavioral evidence shows that conflict control occurs over successive trials as well as longer time scales of trial blocks, but the relation among time scales as well as their neural mechanisms are unclear. This study used measures of behavior, EEG, and a simple quantitative model to test the hypothesis that conflict control at the block level is not exclusively driven by control adjustments over successive trials. Young adults performed an auditory Simon task, and the base rate of compatible vs. incompatible trials was manipulated in separate blocks (25, 50, 75% compatible). EEG data were analyzed using independent component analysis (ICA) to define cortical mechanisms of any base rate and trial-by-trial sequence effects. Reaction time measures had both sequence and base rate effects. Two fronto-medial ICA components indexed sequence and base rate effects, with specific profiles for evoked potentials and oscillations in the theta and alpha frequency bands. Predictive modeling showed that sequence effects accounted for a minority of the variance on behavioral and ICA measures (all < 36%). The results strongly suggest that the base rate manipulation affected behavior and many neural measures beyond the influence of sequence effects.
Glucocorticoids as regulatory signals during intrauterine development.
Experimental physiology, 2015

What is the topic of this review? This review discusses the role of the glucocorticoids as regulatory signals during intrauterine development. It examines the functional significance of these hormones as maturational, environmental and programming signals in determining offspring phenotype. What advances does it highlight? It focuses on the extensive nature of the regulatory actions of these hormones. It highlights the emerging data that these actions are mediated, in part, by the placenta, other endocrine systems and epigenetic modifications of the genome. Glucocorticoids are important regulatory signals during intrauterine development. They act as maturational, environmental and programming signals that modify the developing phenotype to optimize offspring viability and fitness. They affect development of a wide range of fetal tissues by inducing changes in cellular expression of structural, transport and signalling proteins, which have widespread functional consequences at the whole organ and systems levels. Glucocorticoids, therefore, activate many of the physiological systems that have little function in utero but are vital at birth to replace the respiratory, nutritive and excretory functions previously carried out by the placenta. However, by switching tissues from accretion to differentiation, early glucocorticoid overexposure in response to adverse conditions can programme fetal development with longer term physiological consequences for the adult offspring, which can extend to the next generation. The developmental effects of the glucocorticoids can be direct on fetal tissues with glucocorticoid receptors or mediated by changes in placental function or other endocrine systems. At the molecular level, glucocorticoids can act directly on gene transcription via their receptors or indirectly by epigenetic modifications of the genome. In this review, we examine the role and functional significance of glucocorticoids as regulatory signals during intrauterine development and discuss the mechanisms by which they act in utero to alter the developing epigenome and ensuing phenotype.
Hippocampal Protein Kinase C Gamma Signaling Mediates the Impairment of Spatial Learning and Memory in Prenatally Stressed Offspring Rats.
Neuroscience, 2019

Substantial evidence has demonstrated that prenatal stress (PS) impairs spatial learning and memory in offspring. The neuron-specific protein kinase C gamma (PKCγ) has been proposed to be unique in spatial learning and memory. The present study proposes to determine whether hippocampal PKCγ is involved in the detrimental effects of PS on spatial learning and memory in offspring, and to further explore the effects of PS-induced PKCγ-dependent growth-associated protein 43 (GAP-43) and neurogranin (Ng) phosphorylation alteration on calcium/calmodulin-dependent protein kinase II (CaMKII) activation. Prenatal restraint stress models were established, and lentivirus-mediated overexpression of PKCγ in the hippocampal CA1 area was applied. The results demonstrated that PS impaired spatial learning acquisition and memory retrieval on the Morris Water Maze test, especially in juvenile female rats. Hippocampal PKCγ membrane translocation and cytosolic PKCγ levels were decreased in PS females. The expression of phosphorylated GAP-43 (p-GAP-43) and phosphorylated Ng (p-Ng), as well as phosphorylated CaMKII (p-CaMKII), was significantly reduced in the hippocampus of PS females. Overexpression of PKCγ in the hippocampal CA1 area recovered the ability of spatial learning and memory in PS female offspring. Furthermore, enhancing PKCγ reversed PS-induced membrane and cytosolic PKCγ reduction, and restored levels of GAP-43 and Ng phosphorylation, and CaMKII activation in the hippocampus. In conclusion, PS possibly decreases hippocampal PKCγ activity, resulting in a reduction of p-GAP-43 and p-Ng, which underlies insufficient CaMKII activation, thereby impairing spatial learning and memory.
Rhinal hypometabolism on FDG PET in healthy APO-E4 carriers: impact on memory function and metabolic networks.
European journal of nuclear medicine and molecular imaging, 2015

The ε4 allele of the apolipoprotein E (APO-E4) gene, a genetic risk factor for Alzheimer's disease (AD), also modulates brain metabolism and function in healthy subjects. The aim of the present study was to explore cerebral metabolism using FDG PET in healthy APO-E4 carriers by comparing cognitively normal APO-E4 carriers to noncarriers and to assess if patterns of metabolism are correlated with performance on cognitive tasks. Moreover, metabolic connectivity patterns were established in order to assess if the organization of neural networks is influenced by genetic factors.
Expression profile of the entire family of Adhesion G protein-coupled receptors in mouse and rat.
BMC neuroscience, 2008

The Adhesion G protein-coupled receptors (GPCRs) are membrane-bound receptors with long N termini. This family has 33 members in humans. Several Adhesion GPCRs are known to have important physiological functions in CNS development and immune system response mediated by large cell surface ligands. However, the majority of Adhesion GPCRs are still poorly studied orphans with unknown functions.
Toward personalized cognitive diagnostics of at-genetic-risk Alzheimer's disease.
Proceedings of the National Academy of Sciences of the United States of America, 2019

Spatial navigation is emerging as a critical factor in identifying preclinical Alzheimer's disease (AD). However, the impact of interindividual navigation ability and demographic risk factors (e.g., APOE, age, and sex) on spatial navigation make it difficult to identify persons "at high risk" of AD in the preclinical stages. In the current study, we use spatial navigation big data (
Repetitive eccentric muscle contractions increase torque unsteadiness in the human triceps brachii.
Journal of electromyography and kinesiology : official journal of the International Society of Electrophysiological Kinesiology, 2010

Torque steadiness and low-frequency fatigue (LFF) were examined in the human triceps brachii after concentric or eccentric fatigue protocols. Healthy young males (n=17) performed either concentric or eccentric elbow extensor contractions until the eccentric maximal voluntary torque decreased to 75% of pre-fatigue for both (concentric and eccentric) protocols. The number of concentric contractions was greater than the number of eccentric contractions needed to induce the same 25% decrease in eccentric MVC torque (52.2+/-2.9 vs. 41.5+/-2.1 for the concentric and eccentric protocols, respectively, p<.01). The extent of peripheral fatigue was approximately 12% greater after the concentric compared to the eccentric protocol (twitch amplitude), whereas LFF (increase in double pulse torque/single pulse torque), was similar across protocols. Steadiness, or the ability for a subject to hold a submaximal isometric contraction, was approximately 20 % more impaired during the Ecc protocol (p=.052). Similarly, the EMG activity required to hold the torque steady was nearly 20% greater after the eccentric compared to concentric protocol. These findings support that task dependent eccentric contractions preferentially alter CNS control during a precision based steadiness task.
Interpreting neuroticism scores across the adult life course: immutable or experience-dependent set points of negative affect?
Clinical psychology review, 2012

Neuroticism (N) scores predict psychopathology. Therefore, it is important to know how to best interpret N-scores. This paper reviews prior interpretations, the item content of N-measures and relevant empirical studies. We propose that N-scores reflect person-specific negative affect set points. We distinguish three possible set point models. (1) The immutable set point model in which N-scores fluctuate with short-term perturbations in reaction to positive and negative experiences but always return to their person-specific set point. (2) The experience-dependent model in which an individual's set point can change during any life stage when prompted by far-reaching experiences. (3) The mixed model, a combination of the first two models, which separates the variation in neuroticism into stable and changing components. The changing component is experience-dependent. Current evidence provides little support for the immutable model. Rather, the evidence, though inconclusive, suggests that the experience-dependent or mixed model may help to explain between- and within-subject differences in N-scores across the life span. In particular, the observation that the differential consistency of N-scores tends to drop over time, but has not been shown to approach zero, is consistent with the mixed model. We discuss implications of the models and how to distinguish them empirically.