rudyvdbrink/CitationDatabase huggingface dataset:
Choice-based assessments outperform traditional measures for chronic depressive-like behaviors in rats after brain injury.
Behavioural brain research, 2020

Depression is the most common psychiatric comorbidity to be diagnosed following traumatic brain injury (TBI). In clinical populations, TBI-induced depression may be particularly difficult to treat due to both unique underlying causes and the propensity for treatment resistance. Preclinical assays are needed to characterize depressive-like behavior in models of TBI and evaluate treatments. In the current study, two traditionally-acute assays of depressive-like behaviors, the Forced Swim Task and Saccharin Preference, were extended longitudinally to evaluate chronic TBI-induced depressive-like behaviors in male rats. Two chronic measures of motivation, the Progressive Ratio (PR) task and Effort Discounting Task (EDT), were also tested. The PR measures motivation to exert effort, while the EDT parametrically evaluates choice between low- and high-effort requirements. The EDT was the only assay which captured chronic depressive-like behavior after TBI, albeit with a degree of recovery over time. We found that traditionally-acute measures (Forced Swim Task, Saccharin Preference), and even our other chronic measure (PR), failed to capture long-term deficits. We also challenged serotonin and dopamine systems (via fluoxetine and bupropion) to evaluate how TBI-induced changes to these systems might drive depressive-like behaviors. Although we found no effect of fluoxetine, high-dose bupropion differentially impaired TBI rats. These findings suggest that (1) TBI-induced depressive symptoms remain difficult to measure at the preclinical level, (2) treatment for TBI-induced depression requires further exploration, and (3) obstacles at the preclinical level may translate to treatment failure at the clinical level.
Restructuring of amygdala subregion apportion across adolescence.
Developmental cognitive neuroscience, 2021

Total amygdala volumes develop in association with sex and puberty, and postmortem studies find neuronal numbers increase in a nuclei specific fashion across development. Thus, amygdala subregions and composition may evolve with age. Our goal was to examine if amygdala subregion absolute volumes and/or relative proportion varies as a function of age, sex, or puberty in a large sample of typically developing adolescents (N = 408, 43 % female, 10-17 years). Utilizing the in vivo CIT168 atlas, we quantified 9 subregions and implemented Generalized Additive Mixed Models to capture potential non-linear associations with age and pubertal status between sexes. Only males showed significant age associations with the basolateral ventral and paralaminar subdivision (BLVPL), central nucleus (CEN), and amygdala transition area (ATA). Again, only males showed relative differences in the proportion of the BLVPL, CEN, ATA, along with lateral (LA) and amygdalostriatal transition area (ASTA), with age. Using a best-fit modeling approach, age, and not puberty, was found to drive these associations. The results suggest that amygdala subregions show unique variations with age in males across adolescence. Future research is warranted to determine if our findings may contribute to sex differences in mental health that emerge across adolescence.
The essential inner membrane protein YejM is a metalloenzyme.
Scientific reports, 2020

Recent recurrent outbreaks of Gram-negative bacteria show the critical need to target essential bacterial mechanisms to fight the increase of antibiotic resistance. Pathogenic Gram-negative bacteria have developed several strategies to protect themselves against the host immune response and antibiotics. One such strategy is to remodel the outer membrane where several genes are involved. yejM was discovered as an essential gene in E. coli and S. typhimurium that plays a critical role in their virulence by changing the outer membrane permeability. How the inner membrane protein YejM with its periplasmic domain changes membrane properties remains unknown. Despite overwhelming structural similarity between the periplasmic domains of two YejM homologues with hydrolases like arylsulfatases, no enzymatic activity has been previously reported for YejM. Our studies reveal an intact active site with bound metal ions in the structure of YejM periplasmic domain. Furthermore, we show that YejM has a phosphatase activity that is dependent on the presence of magnesium ions and is linked to its function of regulating outer membrane properties. Understanding the molecular mechanism by which YejM is involved in outer membrane remodeling will help to identify a new drug target in the fight against the increased antibiotic resistance.
Hippocampal and anterior cingulate cortex contribution to the processing of recently-acquired and remotely stored spatial memories in rats trained during preadolescence.
Neurobiology of learning and memory, 2020

Preadolescent development is characterized by a reorganization of connectivity within and between brain regions that coincides with the emergence of more complex behaviors. The hippocampus is one such region that undergoes extensive preadolescent remodeling and as this process continues, spatial memory functions emerge. The current work investigated whether preadolescent spatial memories persist beyond 24 h and stabilize into the postadolescent period as remote memories supported by cortical networks in the anterior cingulate cortex (ACC). Male Long Evans rats were trained on the Morris water maze at different time frames from postnatal day (P) 18-26 and compared to P50 rats. Testing occurred at either a recent (24 h) or remote (3 weeks) timepoint. Spatial learning was evident in all age groups (P18, P20, P22, P24 and P50) across the 3 training days but only the P22 and P24 groups showed spatial learning that matched the P50 group. In light of this, the only group to show intact remote (3 week) memory was the P50 group. Spaced training in the P18 group did not improve retention at the recent or remote testing intervals. The P18 and P50 groups tested at 24 h showed more CA1 hippocampal c-Fos labeling than groups tested at 3 weeks. The P50 group tested at 3 weeks showed elevated c-Fos labeling in the anterior cingulate (ACC) compared to the P18 group tested at 3 weeks and the P50 group tested at 24 h. Spaced training in the P18 group was associated with elevated c-Fos labeling in the ACC at the 3-week test. Groups trained at P20, 22, and 24 showed more c-Fos labelling in the ACC than in the CA1. Results suggest that while spatial information processing emerges around P18/P20, remote spatial retention and the neural substrates that support retention are not in place until after P26 in rats.
Default-mode network activation underlies accurate contextual processing of exclusive disjunctions in older but not younger adults.
NeuroImage, 2019

Young adults proactively engage frontoparietal processing of contextual cues to preempt subsequent events. Rather than being preemptive, older adults engage these brain areas reactively upon event occurrences. Reactive frontoparietal processes in older adults, however, might be insufficient for complex contextual neural computations where utilities of contexts are not straightforward but dependent on a set of stimulus-response rules. Applying non-linear logic (XOR) rules in an fMRI experiment, we found higher default-mode network (DMN) activity critical for correctly responding to such contingency in older but not younger adults. Moreover, older individuals with higher proactive cue processing showed better performances with less DMN activity. Thus, DMN processing provides critical support when older adults are faced with complex contextual contingencies. These findings suggest an age-related change in the neurocomputational role of introspective processes in decision-making from young to older adulthood.
Development of the AANS/CNS joint Section on Tumors: challenges and opportunities.
Journal of neuro-oncology, 2014

Over the 30 years since the formation of the AANS/CNS Section on Tumors, the breadth and scope of our activities have continued to expand. An initial focus on education and collaboration between those members of the neurosurgical community dedicated to the care of patients with neurological tumors has broadened to include development of international relationships, participation in clinical trials and efforts to define standards of care and quality metrics. As we navigate the rapidly changing environment of health care, the Section on Tumors will occupy a central role in promoting advocacy, establishing collaboration, providing education, and supporting research for the community of neurosurgeons dedicated to the care of patients with neurological tumors. This article will provide an update and status report on the development of the Section on Tumors, followed by a brief discussion of the challenges and opportunities emerging at the onset of our fourth decade of service.
Phase imaging with multiple phase-cycled balanced steady-state free precession at 9.4 T.
NMR in biomedicine, 2017

While phase imaging with a gradient echo (GRE) sequence is popular, phase imaging with balanced steady-state free precession (bSSFP) has been underexplored. The purpose of this study was to investigate anatomical and functional phase imaging with multiple phase-cycled bSSFP, in expectation of increasing spatial coverage of steep phase-change regions of bSSFP. Eight different dynamic 2D pass-band bSSFP studies at four phase-cycling (PC) angles and two T
Losing control: Mostly incongruent lists postpone, but do not eliminate, the Stroop effect.
Attention, perception & psychophysics, 2018

Identifying the color (e.g., red) of a color word that is incongruent (e.g., "BLUE") can be remarkably difficult. The Stroop effect is a measure of the interference between activity from word and color pathways. The efficacy of these pathways is thought to be highly contingent on the frequency of incongruent trials within a block. A block of trials with mostly incongruent (MI) trials typically results in a smaller Stroop effect than a block with mostly congruent (MC) trials. This reduction of the Stroop effect has been largely attributed to the strategic list-wide control of the word pathway. Here, the time course of the Stroop effect was explored using speed-accuracy tradeoff functions (SATfs) in tasks with 50 % congruent, MC, and MI trials. In the MC and 50 % congruent condition, color-word congruency affected the rate parameter of the SATf. In the MI condition, however, congruency affected the asymptote. This evidence is consistent with the idea that the strategic control of the word pathway is an effortful, temporary phenomenon, prone to buckle if responding is held in check.
The perception of text triggers reflexive oculomotor orienting.
Cortex; a journal devoted to the study of the nervous system and behavior, 2018

As you read this text, your brain is busy integrating numerous different processes-perceptual, cognitive and motor. While you acquire the semantic and linguistic contents of this abstract, your eyes traverse its lines with speed and coordination. The oculomotor response to text is so rapid and precise that it is hypothesized it to be partially based on reflexive orienting mechanisms. In this study we examined the hypothesis that the presentation of written text triggers reflexive orienting toward the direction of reading, similarly to the effect of peripheral stimulation or that of symbolic directional cues (arrows or gazing eyes). In three experiments, participants (N = 120) were presented with task-irrelevant text, shortly followed by a left/right pro-saccade task. The first experiment confirmed the hypothesis by showing that saccades which are congruent with the direction of reading are faster than those which are incongruent. This was observed both in right-to-left (Hebrew) and in left-to-right (English) reading-systems and similarly in native-Hebrew and native-English readers. A second experiment showed that this directional bias is found not only for readable text but also for meaningless strings of letters. This confirmed that the bias is driven pre-reading non-lexical processes. The third experiment examined the time-course of this effect. We conclude that text-perception actives early reflexive eye-movements programs and suggest that this link is an essential building-block of fast and effortless reading.
Blood-Brain Barrier Permeability of Asiaticoside, Madecassoside and Asiatic Acid in Porcine Brain Endothelial Cell Model.
Journal of pharmaceutical sciences, 2021

Neurotherapeutic potentials of Centella asiatica and its reputation to boost memory, prevent cognitive deficits and improve brain functions are widely acknowledged. The plant's bioactive compounds, i.e. asiaticoside, madecassoside and asiatic acid were reported to have central nervous system (CNS) actions, particularly in protecting the brain against neurodegenerative disorders. Hence, it is important for these compounds to cross the blood-brain barrier (BBB) to be clinically effective therapeutics. This study aimed to explore the capability of asiaticoside, madecassoside and asiatic acid to cross the BBB using in vitro BBB model from primary porcine brain endothelial cells (PBECs). Our findings showed that asiaticoside, madecassoside and asiatic acid are highly BBB permeable with apparent permeability (P
Absence of Neuronal Response Modulation with Familiarity in Perirhinal Cortex.
Neuroscience, 2018

The perirhinal cortex (PRH) is considered a crucial cortical area for familiarity memory and electrophysiological studies have reported the presence of visual familiarity encoding neurons in PRH. However, recent evidence has questioned the existence of these neurons. Here, we used a visual task in which head-restrained mice were passively exposed to oriented gratings or natural images. Evoked potentials and single-unit recordings showed evoked responses to novelty in V1 under some conditions. However, the PRH showed no response modulation with respect to familiarity under a variety of different conditions or retention delays. These results indicate that the PRH does not contribute to familiarity/novelty encoding using passively exposed visual stimuli.
Descending Dopaminergic Inputs to Reticulospinal Neurons Promote Locomotor Movements.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 2020

Meso-diencephalic dopaminergic neurons are known to modulate locomotor behaviors through their ascending projections to the basal ganglia, which in turn project to the mesencephalic locomotor region, known to control locomotion in vertebrates. In addition to their ascending projections, dopaminergic neurons were found to increase locomotor movements through direct descending projections to the mesencephalic locomotor region and spinal cord. Intriguingly, fibers expressing tyrosine hydroxylase (TH), the rate-limiting enzyme of dopamine synthesis, were also observed around reticulospinal neurons of lampreys. We now examined the origin and the role of this innervation. Using immunofluorescence and tracing experiments, we found that fibers positive for dopamine innervate reticulospinal neurons in the four reticular nuclei of lampreys. We identified the dopaminergic source using tracer injections in reticular nuclei, which retrogradely labeled dopaminergic neurons in a caudal diencephalic nucleus (posterior tuberculum [PT]). Using voltammetry in brain preparations isolated
Brain PUFA Concentrations Are Differentially Affected by Interactions of Diet, Sex, Brain Regions, and Phospholipid Pools in Mice.
The Journal of nutrition, 2020

PUFAs play vital roles in the development, maintenance, and functioning of circuitries that regulate reward and social behaviors. Therefore, modulations in PUFA concentrations of these brain regions may disrupt reward and social circuitries contributing to mood disorders, developmental disabilities, and addictions. Though much is known about regional and phospholipid-pool-specific PUFA concentrations, less is known about the effects of dietary interventions that concurrently lowers n-6 PUFA and supplements n-3 PUFA, on brain PUFA concentrations. There is even less knowledge on the effects of sex on brain PUFA concentrations.
Psychometric Properties of the Persian Version of the God Locus of Health Control (GLHC): A Study on Muslim Pilgrims.
Journal of religion and health, 2018

God Locus of Health Control (GHLC) scale is an instrument for assessing to what extent one believes that God controls his/her health or disease. The aim of this study was to describe the cultural adaptation of this scale and to investigate the psychometric properties of Persian version. At first, the scale was forward-translated to Persian, and then, backward-translation was conducted. Finally, the synthesized back-translated version was prepared and compared to the draft Persian-language version of the GLHC. In the next step, in order to test cognitive debriefing, the Persian-language version of the questionnaire was completed by 20 pilgrims. Final version of Persian GHLC scale was answered by 600 pilgrims attending religious holy places. Mean age of respondents was 33.15 (±11.04) years. There was a positive correlation between six items of GLHC scale and inter-item correlation coefficients. The Cronbach's alpha coefficient was 0.84. Exploratory factor analysis showed that the G LHC scale had a one-factor structure. The factor loadings for all items ranged from 0.57 to 0.77. Residual correlations of all items were between -0.2 and +0.2. The people with lower income had significantly higher score of GLHC scale. GLHC scale score had a significant positive correlation with DUREL subscale and MHLC Chance Externality subscale score. There was no a significant correlation between GLHC scale score and Multidimensional Health Locus of Control (MHLC) Internality and Powerful subscales scores. The results of the present study showed that the Persian version of GHLC scale has acceptable factorial validity and internal consistency reliability.
Effect of TTX suppression of hippocampal activity following status epilepticus.
Seizure, 2008

Status epilepticus (SE) is a severe neurological condition that can result in brain damage. In animals, SE is associated with cell loss and aberrant synaptogenesis. These pathological processes appear to be activity-dependent and may continue after the SE has ended. We postulated that suppression of electrical activity following SE at the site of the epileptic focus will reduce seizure-induced damage. To achieve this goal, tetrodotoxin (TTX) was used to suppress electrical activity in the hippocampi bilaterally following SE. Adult rats experienced lithium-pilocarpine-induced SE for 2h while controls underwent sham-SE with saline injections. Starting 12h after the SE or sham-SE rats received either continuous TTX (1 microM) or saline infusions through cannulas implanted in the bilateral hippocampi for 5h daily for 4 days. TTX resulted in significant EEG suppression and reduction in spikes and sharp waves. Rats were sacrificed 2 weeks after SE and the brains examined for cell loss and sprouting. Rats receiving TTX following SE had significantly more cell loss as well as a trend toward more mossy fiber sprouting than saline-treated rats following SE. TTX injection in sham-SE rats caused no cell loss or mossy fiber sprouting. These results suggest that suppression of electrical activity following SE is detrimental.
Face perception enhances insula and motor network reactivity in Tourette syndrome.
Brain : a journal of neurology, 2018

Tourette syndrome is a neurodevelopmental disorder, characterized by motor and phonic tics. Tics are typically experienced as avolitional, compulsive, and associated with premonitory urges. They are exacerbated by stress and can be triggered by external stimuli, including social cues like the actions and facial expressions of others. Importantly, emotional social stimuli, with angry facial stimuli potentially the most potent social threat cue, also trigger behavioural reactions in healthy individuals, suggesting that such mechanisms may be particularly sensitive in people with Tourette syndrome. Twenty-one participants with Tourette syndrome and 21 healthy controls underwent functional MRI while viewing faces wearing either neutral or angry expressions to quantify group differences in neural activity associated with processing social information. Simultaneous video recordings of participants during neuroimaging enabled us to model confounding effects of tics on task-related responses to the processing of faces. In both Tourette syndrome and control participants, face stimuli evoked enhanced activation within canonical face perception regions, including the occipital face area and fusiform face area. However, the Tourette syndrome group showed additional responses within the anterior insula to both neutral and angry faces. Functional connectivity during face viewing was then examined in a series of psychophysiological interactions. In participants with Tourette syndrome, the insula showed functional connectivity with a set of cortical regions previously implicated in tic generation: the presupplementary motor area, premotor cortex, primary motor cortex, and the putamen. Furthermore, insula functional connectivity with the globus pallidus and thalamus varied in proportion to tic severity, while supplementary motor area connectivity varied in proportion to premonitory sensations, with insula connectivity to these regions increasing to a greater extent in patients with worse symptom severity. In addition, the occipital face area showed increased functional connectivity in Tourette syndrome participants with posterior cortical regions, including primary somatosensory cortex, and occipital face area connectivity with primary somatosensory and primary motor cortices varied in proportion to tic severity. There were no significant psychophysiological interactions in controls. These findings highlight a potential mechanism in Tourette syndrome through which heightened representation within insular cortex of embodied affective social information may impact the reactivity of subcortical motor pathways, supporting programmed motor actions that are causally implicated in tic generation. Medicinal and psychological therapies that focus on reducing insular hyper-reactivity to social stimuli may have potential benefit for tic reduction in people with Tourette syndrome.
Differential Emergence and Stability of Sensory and Temporal Representations in Context-Specific Hippocampal Sequences.
Neuron, 2020

Hippocampal spiking sequences encode external stimuli and spatiotemporal intervals, linking sequential experiences in memory, but the dynamics controlling the emergence and stability of such diverse representations remain unclear. Using two-photon calcium imaging in CA1 while mice performed an olfactory working-memory task, we recorded stimulus-specific sequences of "odor-cells" encoding olfactory stimuli followed by "time-cells" encoding time points in the ensuing delay. Odor-cells were reliably activated and retained stable fields during changes in trial structure and across days. Time-cells exhibited sparse and dynamic fields that remapped in both cases. During task training, but not in untrained task exposure, time-cell ensembles increased in size, whereas odor-cell numbers remained stable. Over days, sequences drifted to new populations with cell activity progressively converging to a field and then diverging from it. Therefore, CA1 employs distinct regimes to encode external cues versus their variable temporal relationships, which may be necessary to construct maps of sequential experiences.