rudyvdbrink/CitationDatabase huggingface dataset:
Evaluating Visual Cues Modulates Their Representation in Mouse Visual and Cingulate Cortex.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 2021

Choosing an action in response to visual cues relies on cognitive processes, such as perception, evaluation, and prediction, which can modulate visual representations even at early processing stages. In the mouse, it is challenging to isolate cognitive modulations of sensory signals because concurrent overt behavior patterns, such as locomotion, can also have brainwide influences. To address this challenge, we designed a task, in which head-fixed mice had to evaluate one of two visual cues. While their global shape signaled the opportunity to earn reward, the cues provided equivalent local stimulation to receptive fields of neurons in primary visual (V1) and anterior cingulate cortex (ACC). We found that mice evaluated these cues within few hundred milliseconds. During this period, ∼30% of V1 neurons became cue-selective, with preferences for either cue being balanced across the recorded population. This selectivity emerged in response to the behavioral demands because the same neurons could not discriminate the cues in sensory control measurements. In ACC, cue evaluation affected a similar fraction of neurons; emerging selectivity, however, was stronger than in V1, and preferences in the recorded population were biased toward the cue promising reward. Such a biased selectivity regime might allow the mouse to infer the promise of reward simply by the overall level of activity. Together, these experiments isolate the impact of task demands on neural responses in mouse cerebral cortex, and document distinct neural signatures of cue evaluation in V1 and ACC.
Nna1 gene deficiency triggers Purkinje neuron death by tubulin hyperglutamylation and ER dysfunction.
JCI insight, 2020

Posttranslational glutamylation/deglutamylation balance in tubulins influences dendritic maturation and neuronal survival of cerebellar Purkinje neurons (PNs). PNs and some additional neuronal types degenerate in several spontaneous, independently occurring Purkinje cell degeneration (pcd) mice featuring mutant neuronal nuclear protein induced by axotomy (Nna1), a deglutamylase gene. This defective deglutamylase allows glutamylases to form hyperglutamylated tubulins. In pcd, all PNs die during postnatal "adolescence." Neurons in some additional brain regions also die, mostly later than PNs. We show in laser capture microdissected single PNs, in cerebellar granule cell neuronal clusters, and in dissected hippocampus and substantia nigra that deglutamase mRNA and protein were virtually absent before pcd PNs degenerated, whereas glutaminase mRNA and protein remained normal. Hyperglutamylated microtubules and dimeric tubulins accumulated in pcd PNs and were involved in pcd PN death by glutamylase/deglutamylase imbalance. Importantly, treatment with a microtubule depolymerizer corrected the glutamylation/deglutamylation ratio, increasing PN survival. Further, before onset of neuronal death, pcd PNs displayed prominent basal polylisosomal masses rich in ER. We propose a "seesaw" metamorphic model summarizing mutant Nna1-induced tubulin hyperglutamylation, the pcd's PN phenotype, and report that the neuronal disorder involved ER stress, unfolded protein response, and protein synthesis inhibition preceding PN death by apoptosis/necroptosis.
Cervical Laminoplasty for Resection of Hemorrhagic Cavernous Malformation Using a Biportal Technique.
World neurosurgery, 2020

This Video 1 presents the surgical management of a 36-year-old woman who presented with progressive weakness in her right arm associated with a pins-and-needles sensation. Magnetic resonance imaging of the cervical spine revealed a likely hemorrhagic cavernous malformation of the spinal cord at the C3-4 level. The lesion was wholly intramedullary with no presentation to the surface of the spinal cord. It was located in the spinal cord centrally with some right-side predominance. Treatment options were presented to the patient, who agreed to surgery. A resection was performed after a hemilaminotomy at C3 and C4 levels. A biportal technique was used, demonstrating resection of the malformation through 2 small myelotomies made between the entering rootlets at the dorsal root entry zone. This was intended to preserve all roots at the entry zone while enabling visualization of the entire cavernous malformation and its cavity from 2 different portals of entry, essentially providing the same field of access while preserving all roots. After successful resection, the cavity was carefully inspected and closure of the dura was performed, followed by fixation of the osteotomy sites of the laminae at C3 and C4. The patient awoke with no new neurologic deficits and has had no evidence of lesion recurrence or symptoms in 3-year follow-up. The patient provided consent for publication.
Sex-related human brain asymmetry in hemispheric functional gradients.
NeuroImage, 2021

The left and right hemispheres of the human brain are two connected but relatively independent functional modules; they show multidimensional asymmetries ranging from particular local brain unit properties to entire hemispheric connectome topology. To date, however, it remains largely unknown whether and how hemispheric functional hierarchical structures differ between hemispheres. In the present study, we adopted a newly developed resting-state (rs) functional connectivity (FC)-based gradient approach to evaluate hemispheric functional hierarchical structures and their asymmetries in right-handed healthy young adults. Our results showed an overall mirrored principal functional gradient between hemispheres, with the sensory cortex and the default-mode network (DMN) anchored at the two opposite ends of the gradient. Interestingly, the left hemisphere showed a significantly larger full range of the principal gradient in both males and females, with males exhibiting greater leftward asymmetry. Similarly, the principal gradient component scores of two regions around the middle temporal gyrus and posterior orbitofrontal cortex exhibited similar hemisphere × sex interaction effects: a greater degree of leftward asymmetry in males than in females. Moreover, we observed significant main hemisphere and sex effects in distributed regions across the entire hemisphere. All these results are reproducible and robust between test-retest rs-fMRI sessions. Our findings provide evidence of functional gradients that enhance the present understanding of human brain asymmetries in functional organization and highlight the impact of sex on hemispheric functional gradients and their asymmetries.
Convergent evidence for a theory of rapid, automatic, and accurate sex ratio tracking.
Acta psychologica, 2020

It is presumed that people track the sex ratios in their environment (the number of males relative to number of females) in order to adaptively adjust their decisions and behaviors, but this actual tracking ability has not been established. The relevance of sex ratio information, drawn from evolutionary biology and studies of human relationship decision making, is integrated here with memory research (on frequency encoding), perception research (on ensemble coding), and neuroscience research. A series of four experiments provide empirical results to help fill research gaps and facilitate this theoretical integration. In particular, these studies connect details from memory research on relatively automatic frequency encoding of both items and categories, perception research on summary statistics from ensemble coding, and theoretical ideas about the function of these abilities (specifically applied to human sex ratios based on faces) from social and evolutionary approaches. Collectively this research demonstrates an evolved psychological mechanism for functional, fast, and relatively automatic human abilities to track experienced sex ratios in the social world. This sex ratio information is theorized to underpin documented facultative adjustments in relationship dynamics as well as perceptions of social group characteristics. This integrative approach highlights how the coding, memory, and judgments about population sex ratios can both account for a number of existing findings and point towards key further research.
Impact of Dementia-Related Psychosis on Patients and Caregivers: The Treatment Imperative.
The Journal of clinical psychiatry, 2020

​​​​​​​​ Behavioral and psychological symptoms are common in patients with dementia. Especially troublesome are the delusions and hallucinations of dementia-related psychosis (DRP) due to their negative impact on both patients and their carers and family members. This report reviews the impact of DRP on patients and carers, assessment tools, and coping strategies and techniques to help care partners manage DRP.
Ten-year follow-up after Gamma Knife radiosurgery of meningioma and review of the literature.
Acta neurochirurgica, 2020

With regard to the generally slow growth of meningioma, it is essential to analyse clinical treatment results in a long-term perspective. The purpose of the present analysis is to provide clinical data after Gamma Knife radiosurgery of meningioma in a 10-year perspective together with a review of the current literature.
HFD-induced hepatic lipid accumulation and inflammation are decreased in Factor D deficient mouse.
Scientific reports, 2020

Excessive intake of fat causes accumulation of fat in liver, leading to non-alcoholic fatty liver disease (NAFLD). High-fat diet (HFD) upregulates the expression of Factor D, a complement pathway component, in the liver of mice. However, the functions of Factor D in liver are not well known. Therefore, the current study investigated the relationship between Factor D and hepatic lipid accumulation using CRISPR/Cas9-mediated Factor D knockout (FD-KO) mice. Factor D deficiency downregulated expression of genes related to fatty acid uptake and de novo lipogenesis in the liver. Furthermore, Factor D deficiency reduced the expression of inflammatory factors (Tnf and Ccl2) and fibrosis markers and decreased accumulation of F4/80-positive macrophages. These data suggest that the Factor D deficiency improved hepatic lipid accumulation and hepatic inflammation in HFD-fed mice.
Belief in unconscious repressed memory is widespread: A comment on Brewin, Li, Ntarantana, Unsworth, and McNeilis (2019).
Journal of experimental psychology. General, 2020

What does believing in repressed memory mean? In a recent article in this journal, Brewin, Li, Ntarantana, Unsworth, and McNeilis (2019, Study 3) argued that when people are asked to indicate their belief in repressed memory, they might actually think of deliberate memory suppression rather than unconscious repressed memory. They further argued that in contrast to belief in unconscious repressed memory, belief in deliberate memory suppression is not scientifically controversial. In this commentary, we show that they are incorrect on both counts. Although Brewin and colleagues surveyed people to indicate their belief in deliberate memory suppression, they neglected to ask their participants whether they (also) believed in unconscious repressed memory. We asked people from the general population whether they believed that traumatic experiences can be unconsciously repressed for many years and then recovered. In 2 studies of the general population, we found high endorsement rates (Study 1 [N = 230]: 59.2% [n = 45]; Study 2 [N = 79]: 67.1% [n = 53]) of the belief in unconscious repressed memory. These endorsement rates did not statistically differ from endorsement rates to statements on repressed memory and deliberate memory suppression. In contrast to what Brewin et al. argued, belief in unconscious repressed memory among lay people is alive and well. Finally, we contend that Brewin et al. overstated the scientific evidence bearing on deliberate repression (suppression). (PsycInfo Database Record (c) 2020 APA, all rights reserved).
Complex Photophysical Properties of K114 Make for a Versatile Fluorescent Probe for Amyloid Detection.
ACS chemical neuroscience, 2021

Protein aggregation is a hallmark of Alzheimer's disease (AD) and many other neurodegenerative disorders. Small organic fluorophores such as Congo Red preferentially bind to cross-β-sheet-rich deposits and have been used to label amyloid plaques and tau tangles in histological samples. However, distinguishing between different conformations of protein aggregates is not trivial. Using silkworm and spider silks (prototypical amyloids) and transgenic AD mouse (5XFAD) and human AD brain samples, we report how spectral confocal microscopy allowed for improved detection and differentiation of protein aggregates based on the unexpected photophysical behavior of the amyloid-specific dye K114. The pH and excitation power had pronounced effects on the emission spectrum and intensity of amyloid-bound K114 fluorescence. When bound to β-sheet-rich assemblies, the emission spectrum of K114 was governed by the local pH of the binding pockets much more than by the pH of the mounting medium, likely due to ionization of titratable phenols. Unexpectedly, exposure to high excitation power caused a permanent increase in fluorescence intensity and a spectral blue-shift. These light-induced fluorescence changes were dependent in a complex manner on laser power, exposure time, pH, and amyloid type examined. The above-mentioned phenomena were observed in silk fibers and Alzheimer brain sections from mouse and human, indicating that this may be a general characteristic of K114 when bound to tightly aggregated macromolecules. Potential mechanisms are discussed, likely involving photoinduced electron transfer. Our findings illustrate how the complex photophysical behavior of amyloid-bound K114 can be exploited for improved detection and differentiation of protein aggregates.
Modelling Learning and Memory in Drosophila to Understand Intellectual Disabilities.
Neuroscience, 2020

Neurodevelopmental disorders (NDDs) include a large number of conditions such as Fragile  X  syndrome, autism spectrum disorders and Down syndrome, among others. They are characterized by limitations in adaptive and social behaviors, as well as intellectual disability (ID). Whole-exome and whole-genome sequencing studies have highlighted a large number of NDD/ID risk genes. To dissect the genetic causes and underlying biological pathways, in vivo experimental validation of the effects of these mutations is needed. The fruit fly, Drosophila melanogaster, is an ideal model to study NDDs, with highly tractable genetics, combined with simple behavioral and circuit assays, permitting rapid medium-throughput screening of NDD/ID risk genes. Here, we review studies where the use of well-established assays to study mechanisms of learning and memory in Drosophila has permitted insights into molecular mechanisms underlying IDs. We discuss how technologies in the fly model, combined with a high degree of molecular and physiological conservation between flies and mammals, highlight the Drosophila system as an ideal model to study neurodevelopmental disorders, from genetics to behavior.
Biology and Bias in Cell Type-Specific RNAseq of Nucleus Accumbens Medium Spiny Neurons.
Scientific reports, 2019

Subcellular RNAseq promises to dissect transcriptional dynamics but is not well characterized. Furthermore, FACS may introduce bias but has not been benchmarked genome-wide. Finally, D1 and D2 dopamine receptor-expressing medium spiny neurons (MSNs) of the nucleus accumbens (NAc) are fundamental to neuropsychiatric traits but have only a short list of canonical surface markers. We address these gaps by systematically comparing nuclear-FACS, whole cell-FACS, and RiboTag affinity purification from D1- and D2-MSNs. Using differential expression, variance partitioning, and co-expression, we identify the following trade-offs for each method. RiboTag-seq best distinguishes D1- and D2-MSNs but has the lowest transcriptome coverage. Nuclear-FACS-seq generates the most differentially expressed genes and overlaps significantly with neuropsychiatric genetic risk loci, but un-annotated genes hamper interpretation. Whole cell-FACS is more similar to nuclear-FACS than RiboTag, but captures aspects of both. Using pan-method approaches, we discover that transcriptional regulation is predominant in D1-MSNs, while D2-MSNs tend towards cytosolic regulation. We are also the first to find evidence for moderate sexual dimorphism in these cell types at baseline. As these results are from 49 mice (n
Metabolic heterogeneity and adaptability in brain tumors.
Cellular and molecular life sciences : CMLS, 2020

The metabolic complexity and flexibility commonly observed in brain tumors, especially glioblastoma, is fundamental for their development and progression. The ability of tumor cells to modify their genetic landscape and adapt metabolically, subverts therapeutic efficacy, and inevitably instigates therapeutic resistance. To overcome these challenges and develop effective therapeutic strategies targeting essential metabolic processes, it is necessary to identify the mechanisms underlying heterogeneity and define metabolic preferences and liabilities of malignant cells. In this review, we will discuss metabolic diversity in brain cancer and highlight the role of cancer stem cells in regulating metabolic heterogeneity. We will also highlight potential therapeutic modalities targeting metabolic vulnerabilities and examine how intercellular metabolic signaling can shape the tumor microenvironment.
Serious Games: Defense Advanced Research Projects Agency: An Interview with Innovation Information Office Program Manager CAPT Russell Shilling, PhD.
Games for health journal, 2012

Dr. Shilling received his Doctor of Philosophy in Experimental Psychology (Auditory Psychophysics/Neuroscience) from the University of North Carolina at Greensboro in 1992 and his Bachelor of Arts in Psychology at Wake Forest University in 1985. In 1993 he graduated with top honors from Aerospace Experimental Psychologist training-Naval Flight Surgeon Class 93002. In 1996, he served as an Assistant/Associate Professor at the U.S. Air Force Academy in the Department of Behavioral Sciences and Leadership. In 2000, he arrived at the Naval Postgraduate School as an Associate Professor in the Operations Research and Systems Engineering Departments and became Technical Director for Immersive Technologies in the Modeling, Virtual Environments, and Simulation Institute. His military decorations include the Defense Superior Service Medal, Meritorious Service Medal (three awards), the Navy Commendation Medal, and the Navy Achievement Medal. He is also the recipient of the American Hospital Association's 2009 Executive Award for Excellence.
Incorporation of Planar Blocks into Twisted Skeletons: Boosting Brightness of Fluorophores for Bioimaging beyond 1500 Nanometer.
ACS nano, 2020

The brightness of organic fluorescence materials determines their resolution and sensitivity in fluorescence display and detection. However, strategies to effectively enhance the brightness are still scarce. Conventional planar π-conjugated molecules display excellent photophysical properties as isolated species but suffer from aggregation-caused quenching effect when aggregated owing to the cofacial π-π interactions. In contrast, twisted molecules show high photoluminescence quantum yield (Φ
Facial emotion recognition impairment is related to disorganisation in multi-episode schizophrenia.
Schizophrenia research. Cognition, 2014

The present investigation explores the relationship between facial emotion recognition (FER) and symptom domains in three groups of schizophrenia spectrum patients (43 ultra-high-risk, 50 first episode and 44 multi-episode patients) in which the existence of FER impairment has already been demonstrated. Regression analysis showed that symptoms and FER impairment are related in multi-episode patients, regardless of the illness duration. We suggest that the link between symptoms and FER impairment is involved in the progression of the disease.
Parvalbumin and Somatostatin Interneurons Contribute to the Generation of Hippocampal Gamma Oscillations.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 2020

γ-frequency oscillations (30-120 Hz) in cortical networks influence neuronal encoding and information transfer, and are disrupted in multiple brain disorders. While synaptic inhibition is important for synchronization across the γ-frequency range, the role of distinct interneuronal subtypes in slow (<60 Hz) and fast γ states remains unclear. Here, we used optogenetics to examine the involvement of parvalbumin-expressing (PV
Seizure control, stress, and access to care during the COVID-19 pandemic in New York City: The patient perspective.
Epilepsia, 2021

Our epilepsy population recently experienced the acute effects of the COVID-19 pandemic in New York City. Herein, we aimed to determine patient-perceived seizure control during the surge, specific variables associated with worsened seizures, the prevalence of specific barriers to care, and patient-perceived efficacy of epilepsy care delivered via telephone and live video visits during the pandemic.
Aspiration fuels willpower: Evidence from the addiction literature.
The Behavioral and brain sciences, 2021

Ainslie identifies two possible motivational sources for resolve: "thinking categorically" and "intertemporal bargaining." Ainslie opts for intertemporal bargaining, adding that thinking categorically has no motivational power. The most researched instance of willpower is remission from addiction. This literature shows that aspirations for a more desirable identity and comfortable lifestyle motivate remission. In other words, "thinking categorically" drives willpower.
Vasculogenesis from Human Dental Pulp Stem Cells Grown in Matrigel with Fully Defined Serum-Free Culture Media.
Biomedicines, 2020

The generation of vasculature is one of the most important challenges in tissue engineering and regeneration. Human dental pulp stem cells (hDPSCs) are some of the most promising stem cell types to induce vasculogenesis and angiogenesis as they not only secrete vascular endothelial growth factor (VEGF) but can also differentiate in vitro into both endotheliocytes and pericytes in serum-free culture media. Moreover, hDPSCs can generate complete blood vessels containing both endothelial and mural layers in vivo, upon transplantation into the adult brain. However, many of the serum free media employed for the growth of hDPSCs contain supplements of an undisclosed composition. This generates uncertainty as to which of its precise components are necessary and which are dispensable for the vascular differentiation of hDPSCs, and also hinders the transfer of basic research findings to clinical cell therapy. In this work, we designed and tested new endothelial differentiation media with a fully defined composition using standard basal culture media supplemented with a mixture of B27, heparin and growth factors, including VEGF-A165 at different concentrations. We also optimized an in vitro Matrigel assay to characterize both the ability of hDPSCs to differentiate to vascular cells and their capacity to generate vascular tubules in 3D cultures. The description of a fully defined serum-free culture medium for the induction of vasculogenesis using human adult stem cells highlights its potential as a relevant innovation for tissue engineering applications. In conclusion, we achieved efficient vasculogenesis starting from hDPSCs using serum-free culture media with a fully defined composition, which is applicable for human cell therapy purposes.