Evaluation of CYT-108, a Recombinant Protease Inhibitor, for Treatment of Mild to Moderate Primary Osteoarthritis of the Knee
Status: Not yet recruiting

The goal of this Phase 1 study is to investigate the safety of CYT-108, our experimental recombinant protease inhibitor drug candidate for osteoarthritis, in a population of patients suffering from osteoarthritis of the knee. The main questions this study aims to answer are:

are two doses of CYT-108, delivered 12 weeks apart, safe when injected directly into the joint?
does administration of CYT-108 result in a reduction in pain, stiffness, and improvement in daily physical function?

Participants will either receive a placebo (Phosphate Buffer Saline, PBS) or CYT-108, and will be asked to report their pain/stiffness at weeks 1, 4, 8, 12, 16, and 26 (in a physician's office) after the initial injection, using a questionnaire to be provided by the physician. In addition to reporting the magnitude of pain, participants will also be asked about the onset of pain reduction. Researchers will compare the pain/stiffness scores between the CYT-108-treated to placebo-treated groups along the 26 week duration of the study. Participants will also receive blood draws along the course of the study, and researchers will analyze this blood for the presence of cartilage degradation product in attempt to identify "disease modification" (i.e., a reduction in cartilage degradation) in response to CYT-108 treatment.

Eligibility:
- EligibilityCriteria: Inclusion Criteria:  Participant with mild to moderate primary knee OA. Provides signed written informed consent before any study procedure is performed. Is willing and able to complete effectiveness and safety questionnaires and can read and understand study instructions. Adult aged 18 years or older at the time of informed consent. Participants of childbearing potential must have a negative serum pregnancy test at screening and negative urine pregnancy test pre-dose on Day 1, and must agree to remain sexually abstinent, or use medically effective contraception (refer to Appendix 14.2) or have a partner who is sterile or same sex, from Screening until at least 3 months after the last treatment. Participants must not be planning to father children or donate sperm from Screening until at least 3 months after the last treatment. Male participants must agree to use a double method of contraception to prevent partner's pregnancy during and 3 months after the last treatment. Male participant should not donate sperm during the same period (for more detail see appendix 14.2). Is ambulatory (assistive devices or knee braces are allowed if used during the study).  Has symptomatic OA in the target knee (one knee) either medial or lateral.  X-ray confirmation of OA at the target knee prior to screening with a grade 2 or 3 score on the K-L grading scale (Kellgren, 1957) using X-ray performed within 6 months of Screening. Knee pain as demonstrated by an average WOMAC pain scale score of 1.5 - 3 at Screening and pre-dose Day 1. Remains symptomatic despite having received standard of- care therapy, such as daily doses of NSAID or any pain medication leading to the study screening. Has not received corticosteroid injection within 6 weeks prior to screening. Has not received hyaluronic acid treatment, Platelet rich plasma (PRP) or any other protein based or stem cells treatment, or any investigational drug within 12 weeks prior to screening.  Exclusion Criteria:  Any of the following:  Grade 4 score on the K-L grading scale for the target knee Grade 3 score on the K-L grading scale and exhibits at least one Grade 4 characteristic (large osteophytes, marked narrowing of joint space, severe sclerosis, or definite deformity of bone contour) Acute fracture of the lower limb Participant with OA in both knees. Medical history of severe bone disease (e.g., osteoporosis, osteonecrosis, joint deformity, instability, or septic arthritis). Has large knee joint effusion, clinically defined as an obvious swelling with loss of the medial sulcus as well as a ballotable patella. Has another disease that can affect the health of the knee (e.g., chronic hemochromatosis; sickle cell anemia; arthropathies of systemic diseases such as chondrocalcinosis, gout, pseudogout, psoriasis, hemophilia, and infectious diseases of the joints). Significant joint infection in the target knee or inflammation or skin disorder in the injection area of the target knee. Fibromyalgia, anserine bursitis, lumbar radiculopathy, neurogenic or vascular claudication, vascular insufficiency of lower limbs, or peripheral neuropathy. Patella femoral instability. History of cartilage allograft, autograft or microfracture in the study knee.  Has diseases that may interfere:  Severe infectious disease with or without fever. COVID-19 patients will not be permitted until two negative tests are produced. The history of suffering from migraine headache and requires ongoing pain medications that are prohibited per protocol. Any significant chronic skin disorders, active skin or soft tissue infection that could interfere with the evaluation of the injection site. Malalignment/deformity of the leg Active Chronic Obstructive Pulmonary Disease (COPD) or Asthma that may require periodic treatment with steroids during the study period. Active malignancy receiving treatment, or prior history of any malignancy, except for basal cell carcinoma or squamous cell carcinoma of the skin treated at least 1 year before screening. Psychiatric, neurological disorders, or cognitive impairment severe enough that the individual is unable to provide informed consent, in the judgement of the Investigator. Incarcerated or confined. Has medical-legal, personal injury, ongoing litigation, or a worker's compensation claim(s). Smokers, tobacco users and any nicotine administration through vaping or patches. History of alcohol or drug abuse. Received corticosteroid injection for any indication within 6 weeks prior to screening. Pregnant and breast-feeding participants or planning to get pregnant during and 6 months after the study is completed. Clinically significant abnormal screening laboratory results (other than those related to OA) Clinically significant abnormal vital signs or physical examination findings (other than those related to OA). Received hyaluronic acid treatment, Platelet rich plasma (PRP) or any other protein based or stem cells treatment, or any investigational drug within 12 weeks prior to screening. Any condition that, in the opinion of the Clinical Investigator, might interfere with the evaluation of the study objectives. - HealthyVolunteers: No - Gender: All - MinimumAge: 18 Years - StdAgeList: Adult, Older Adult