Wyoming Administrative Code › Agency 040 - Game and Fish Commission › Sub-Agency 0001 - Game and Fish Commission Regulations › Chapter 51 - PRIVATE FISH HATCHERIES › Appendix I - COLD-BLOODED WILDLIFE FISH HEALTH INSPECTION PROCEDURES

Current through July 13, 2024
Section
				  1. Fish
				  Health Inspection Procedures.
(a) All inspections shall be conducted following sampling and
				  testing procedures and protocols documented as standard operating procedures
				  (SOP) from the Department Fish Health Laboratory.
(b) Approved inspection procedures shall be available as SOP
				  from the Department Fish Health Laboratory upon request.
(c) All fish health inspections at in-state private fish
				  hatcheries shall be completed by an aquatic animal health inspector or fish
				  pathologist except as provided below.
(i) An aquatic laboratory technician is authorized only for
				  compliance collection of non-lethal samples (e.g., ovarian fluids) for a
				  broodstock fish health inspection after the initial lethal and non-lethal
				  sampling has been conducted by an aquatic animal health inspector or fish
				  pathologist.
(d) All fish health inspections at out-of-state private fish
				  hatcheries shall be completed by an aquatic animal health inspector, fish
				  pathologist or United States Department of Agriculture accredited
				  veterinarian.
(e) Determining Sample Size. The minimum sample size for each
				  lot shall be in accordance with a plan which provides at least 95% confidence
				  of detecting a pathogen (see TABLE below). Attribute sampling shall be based
				  upon the assumption of a pathogen's prevalence in facilities (hatcheries) as
				  follows:
Assumed Pathogen Prevalence
								Level
2%
5%
Lot
								Population
Required Sample
								Size
50
50
35
100
75
45
250
110
50
500
130
55
1,000
140
55
2,000
145
60
10,000
145
60
100,000 or greater
150
60
(f) Sample Procedures
(i) Production Fish
(A) When sampling for viruses, all lots including broodstock
				  lots, shall be sampled at the 5% (or lower) APPL. This pertains to lethal
				  sampling of all fish species.
(B) When sampling for the bacterial pathogens
Y.
				  ruckeri
(enteric redmouth) and
A. salmonicida
(furunculosis), the minimum sampling shall be sixty (60) fish from the most
				  susceptible species as determined by the Department on each water supply at
				  each facility.
(C) When sampling for
R. salmoninarium
(bacterial kidney disease), the minimum sampling shall be sixty (60) fish from
				  all lots over six (6) months of age at the time of sampling.
(D) When sampling for
E. ictaluri
(enteric
				  septicaemia of catfish), sampling shall be sixty (60) fish from the lot being
				  tested.
(E) When sampling for the detection of
M.
				  cerebralis
(salmonid whirling disease) at a facility, the minimum
				  sample size shall be sixty (60) fish from the most susceptible lot on each
				  water source, as determined by the Department. Sampled fish shall have been on
				  the same water source for a cumulative minimum of 1,800 Celsius temperature
				  units (CTU=sum of the average daily water temperature in degrees Celsius)
				  exposure at the time of inspection.
(I) The Department may require that certain lots be sampled
				  individually in addition to sampling conducted on each water source (e.g., lots
				  reared in dirt ponds where conditions are conducive to enabling of the parasite
				  life cycle). Fish sampled from individual lots shall have been on the same
				  water source (e.g., in the same pond) for a minimum of 900 CTU.
(ii) Broodstocks
(A) Conduct lethal and nonlethal sampling (e.g., ovarian
				  fluid sampling) of all broodstock lots at the 5% or lower APPL of listed
				  pathogens annually if adequate numbers of broodstock fish are available.
(I) Individuals sampled for ovarian fluids shall not be used
				  to obtain kidney/spleen samples.
(B) Three (3) consecutive, pathogen free, annual fish health
				  inspections shall be required before gametes are used to develop a confined
				  broodstock.
(C) Fish health inspections shall include sampling all age
				  classes of the broodstock population held at the facility.
(iii) Baitfish and Grass Carp
(A) Viral-Sampling of all lots shall occur at the 5% APPL for
				  the following viruses: Viral Hemorrhagic Septicemia (VHS) virus, Infectious
				  Hematopoietic Necrosis (IHN) virus, Infectious Pancreatic Necrosis (IPN) virus
				  and Spring Viremia of Carp (SVC) virus.
(B) Bacterial-Sampling of all lots shall occur at the 5% APPL
				  for
Y. ruckeri
(enteric redmouth) and
A.
				  salmonicida
(furunculosis).
(C) Certified triploid grass carp (
Ctenopharygodon
				  idella
) shall be tested at the 5% APPL for
B.
				  cheilognathi
(Asian tapeworm).