Wisconsin Administrative Code › Department of Natural Resources › NR 800 - Environmental Protection - Water Supply › Chapter NR 809 - Safe Drinking Water › Subchapter I - Maximum Contaminant Levels, Monitoring and Analytical Requirements for Primary Drinking Water Contaminants › Section NR 809.323 - Analytical requirements for groundwater source microbiological contaminants

Current through June 24, 2024
(1) ANALYTICAL METHODS.
(a) A water supplier for a groundwater system
				  subject to the source water monitoring requirements of s.
				  NR
				  809.325(2) shall collect a standard
				  sample volume of at least 100 mL for
E. coli
analysis
				  regardless of the analytical method used.
(b) A water supplier for a groundwater system
				  shall analyze all groundwater source samples, collected under s.
				  NR
				  809.325(2), using one of the
				  analytical methods listed in Table G for the presence of
E.
				  coli
. If the department requires a public water system to be tested
				  for the presence of enterococci or coliphage, the water supplier shall use one
				  of the methods listed in Table G.
(c) Sample volumes of at least 100 mL shall
				  be used for all analyses using the methods in Table G. Analyses shall be
				  conducted in accordance with the documents listed in the footnotes to Table
				  G.
(d) The sample holding time
				  shall not exceed 30 hours. In all cases, samples should be analyzed as soon
				  after collection as possible.
Table G
Analytical Methods for Source Water
					 Monitoring
Fecal indicator
Methodology
Method Citation
E. coli
Colilert 
								  2
9223 B1
Colisure 
									 2
9223 B1
Membrane Filter
Method EPA Method with MI Agar.
16043
m-ColiBlue24 Test 
									 4
E*Colite Test 
									 5
EC-MUG 
									 6
9221 F1
NA-MUG 
									 6
9222 G1
Enterococci
Multiple-Tube Technique.
9230 B1
Membrane Filter Technique.
9230 C1
Membrane Filter Technique.
1600 
									 7
Enterolert 
									 8
Coliphage
Two-Step Enrichment EPA Method Presence-Absence
								  Procedure.
16019
Single Agar Layer Procedure.
160210
Note:
Copies of the documents listed in the
					 footnotes may be obtained from the sources listed in the footnotes. Copies may
					 be inspected at EPA's Drinking Water Docket, EPA West, 1301 Constitution
					 Avenue, NW., Room B102, Washington DC 20460, Telephone: 202-566-2426, or at the
					 National Archives and Records Administration (NARA). For information on the
					 availability of this material at NARA, call 202-741-6030, or go to:
http://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html
.
1 Methods are described in Standard Methods for the
					 Examination of Water and Wastewater 20th edition (1998) and copies may be
					 obtained from the American Public Health Association, 1015 Fifteenth Street,
					 NW., Washington, DC 20005-2605.
2 Medium is available through IDEXX Laboratories, Inc., One
					 IDEXX Drive, Westbrook, Maine 04092.
3 EPA Method 1604: Total Coliforms and Escherichia coli in
					 Water by Membrane Filtration Using a Simultaneous Detection Technique (MI
					 Medium); September 2002, EPA 821-R-02-024. Method is available at
http://www.epa.gov/microbes/documents/1604sp02.pdf
or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW.,
					 Washington, DC 20460.
4 A description of the m-ColiBlue24 Test, "Total Coliforms
					 and E. coli Membrane Filtration Method with m-ColiBlue24 Broth," Method No.
					 10029 Revision 2, August 17, 1999, is available from Hach Company, 100 Dayton
					 Ave., Ames, IA 50010 or from EPA's Water Resource Center (RC-4100T), 1200
					 Pennsylvania Avenue, NW., Washington, DC 20460.
5 A description of the E*Colite Test, "Charm E*Colite
					 Presence/Absence Test for Detection and Identification of Coliform Bacteria and
					 Escherichia coli in Drinking Water," January 9, 1998, is available from Charm
					 Sciences, Inc., 659 Andover St., Lawrence, MA 01843-1032 or from EPA's Water
					 Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC
					 20460.
6 EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be
					 used for E. coli testing step as described in Sec. 141.21(f)(6)(i) or (ii)
					 after use of Standard Methods 9221 B, 9221 D, 9222 B, or 9222 C.
7 EPA Method 1600: Enterococci in Water by Membrane
					 Filtration Using membrane-Enterococcus Indoxyl-[beta]-D-Glucoside Agar (mEI)
					 EPA 821-R- 02-022 (September 2002) is an approved variation of Standard Method
					 9230C. The method is available at
http://www.epa.gov/microbes/documents/1600sp02.pdf
or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW.,
					 Washington, DC 20460.
8 Medium is available through IDEXX Laboratories, Inc., One
					 IDEXX Drive, Westbrook, Maine 04092. Preparation and use of the medium is set
					 forth in the article "Evaluation of Enterolert for Enumeration of Enterococci
					 in Recreational Waters," by Budnick, G.E., Howard, R.T., and Mayo, D.R., 1996,
					 Applied and Environmental Microbiology, 62:3881-3884.
9 EPA Method 1601: Male-specific (F+) and Somatic Coliphage
					 in Water by Two-step Enrichment Procedure; April 2001, EPA 821-R-01-030. Method
					 is available at
http://www.epa.gov/microbes/documents/1601ap01.pdf
or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW.,
					 Washington, DC 20460.
10 EPA Method 1602: Male-specific (F+) and Somatic
					 Coliphage in Water by Single Agar Layer (SAL) Procedure; April 2001, EPA
					 821-R-01-029. Method is available at
http://www.epa.gov/microbes/documents/1602ap01.pdf
or from EPA's Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW.,
					 Washington, DC 20460.