Rhode Island Code of Regulations › Title 216 - Department of health › Chapter 50 - Environmental health › Subchapter 05 - Water Quality › Part 1 - Public Drinking Water (216-RICR-50-05-1) › Section 216-RICR-50-05-1.21 - Appendix 1

Current through June 14, 2024
A. MICROBIOLOGY
1. Microbiological Testing - Analytical
				  Methodology. Reference for §
											1.6   of this Part- Surface
				  Water and Groundwater Under the Influence of Surface Water and
				  §§1.16.4 and 1.17 of this Part- Distribution Samples including
				  Storage Facilities and Groundwater Sources Regulation.
a. The standard sample volume required for
				  total coliform analysis, regardless of analytical method used, is 100
				  ml.
b. PWS need only determine the
				  presence or absence of total coliforms; a determination of total coliform
				  density is not required.
c. PWS
				  must conduct total coliform analyses in accordance with one of the analytical
				  methods in the following table.
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d. PWS
				  must conduct analysis of Escherichia coli in accordance with one of the
				  following analytical methods:
(1) Minimal
				  Medium ONPG-MUG (MMO-MUG) Test, as set forth in the article "National Field
				  Evaluation of a Defined Substrate Method for the Simultaneous Detection of
				  Total Coliforms and Escherichia coli from Drinking Water: Comparison with
				  Presence-Absence Techniques" (Edberg et al.), Applied and Environmental
				  Microbiology, Volume 55, pp. 1003-1008, April 1989. (Note: The Autoanalysis
				  Colilert System is an MMO-MUG test). If the MMO-MUG test is total
				  coliform-positive after a 24-hour incubation, test the medium for fluorescence
				  with a 366-nm ultraviolet light (preferably with a 6-watt lamp) in the dark. If
				  fluorescence is observed, the sample is E. coli-positive. If fluorescence is
				  questionable (cannot be definitively read) after 24 hours incubation, incubate
				  the culture for an additional four hours (but not to exceed 28 hours total),
				  and again test the medium for fluorescence. The MMO-MUG Test with hepes buffer
				  in lieu of phosphate buffer is the only approved formulation for the detection
				  of E. coli.
(2) The Colisure Test.
				  A description of the Colisure Test may be obtained from the Millipore
				  Corporation, Technical Services Department, 80 Ashby Road, Bedford, MA
				  01730.
(3) The membrane filter
				  method with MI agar, a description of which is cited in footnote 6 to the table
				  in §
													1.21(A)(1)(c)
				    of this Part.
(4) E*Colite®
				  Test, a description of which is cited in footnote 10 to the table in §
													1.21(A)(1)(c)
				    of this Part.
(5)
				  m-ColiBlue24®Test, a description of which is cited in footnote 11 to the
				  table in §
													1.21(A)(1)(c)
				    of this Part.
(6) Readycult®
				  Coliforms 100 Presence/Absence Test, a description of which is cited in
				  footnote 13 to the table in §
													1.21(A)(1)(c)   of this Part.
(7) Membrane Filter Technique using
				  Chromocult® Coliform Agar, a description of which is cited in footnote 14
				  to the table in §
													1.21(A)(1)(c)   of this Part.
(8) Colitag®, a description of which is
				  cited in footnote 15 to the table in §
													1.21(A)(1)(c)   of this Part.
e. The following materials are incorporated
				  by reference in §
												1.21   of this Part with the
				  approval of the Director of the Federal Register in accordance with
				   5 U.S.C. 552(a)  and
				   1 C.F.R. Part 51 .
				  Copies of the analytical methods cited in Standard Methods for the Examination
				  of Water and Wastewater (18th, 19th, and 20th editions) may be obtained from
				  the American Public Health Association et al.; 1015 Fifteenth Street, NW.,
				  Washington, DC 20005-2605. Copies of the MMO-MUG Test, as set forth in the
				  article "National Field Evaluation of a Defined Substrate Method for the
				  Simultaneous Enumeration of Total Coliforms and Escherichia coli from Drinking
				  Water: Comparison with the Standard Multiple Tube Fermentation Method" (Edberg
				  et al.) may be obtained from the American Water Works Association Research
				  Foundation, 6666 West Quincy Avenue, Denver, CO 80235. A description of the
				  Colisure Test may be obtained from the Millipore Corp., Technical Services
				  Department, 80 Ashby Road, Bedford, MA 01730. Copies may be inspected at EPA's
				  Drinking Water Docket; 401 M St., SW.; Washington, DC 20460, or at the National
				  Archives and Records Administration (NARA). For information on the availability
				  of this material at NARA, call (202) 741-6030, or go to:
http://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html
.