reglab/statecodes huggingface dataset:
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-670 - Audits

Current through Register Vol. 40, No. 24, July 15, 2024
A. Internal audits.
1. The laboratory shall arrange for annual
					 internal audits to verify that its operations continue to comply with the
					 requirements of the laboratory's quality system. It is the responsibility of
					 the quality assurance officer to plan and organize audits as required by a
					 predetermined schedule and requested by management.
2. Trained and qualified personnel who are,
					 wherever resources permit, independent of the activity to be audited, shall
					 carry out these audits. Personnel shall not audit their own activities except
					 when it can be demonstrated that an effective audit will be carried
					 out.
3. Where the audit findings
					 cast doubt on the correctness or validity of the laboratory's calibrations or
					 test results, the laboratory shall take immediate corrective action.
4. A laboratory may have an audit performed
					 under contract by an outside source competent to audit the laboratory's
					 operations.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-680 - Subcontracting analytical samples

Current through Register Vol. 40, No. 24, July 15, 2024
A.
					 Where a laboratory subcontracts any part of the testing covered under this
					 chapter, the testing shall only be subcontracted to a laboratory certified
					 under 1VAC30-46.
B. The report from
					 the subcontractor shall be a separate part of the laboratory report and
					 identified as laboratory testing done by a subcontractor.
C. The laboratory shall retain records
					 demonstrating that the requirements of this section have been met.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-690 - Outside support services and supplies

Current through Register Vol. 40, No. 24, July 15, 2024
A. The laboratory shall have a policy and
					 procedures for the selection and purchasing of services and supplies it uses
					 that affect the quality of environmental tests. Procedures shall exist for the
					 purchase, reception and storage of reagents and laboratory consumable materials
					 relevant for the environmental tests.
B. The laboratory shall ensure that purchased
					 equipment and consumable materials are not used until they have been inspected,
					 calibrated or otherwise verified as complying with standard specifications or
					 requirements defined in the methods for the tests concerned. These services and
					 supplies used shall comply with specified requirements. Records of actions
					 taken to check compliance shall be maintained.
C. The laboratory shall maintain records of
					 all suppliers from whom it obtains support services or supplies required for
					 tests.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-700 - Complaints

Current through Register Vol. 40, No. 24, July 15, 2024
The laboratory shall have documented policy and
						procedures for the resolution of complaints about the laboratory's activities.
						Where a complaint or any other circumstance raises doubt concerning the
						laboratory's compliance with the laboratory's policies or procedures, or with
						the requirements of this chapter or otherwise concerning the quality of the
						laboratory's calibrations or tests, the laboratory shall ensure that those
						areas of activity and responsibility involved are promptly audited in
						accordance with
						1VAC30-45-670 A. Records of the
						complaint and subsequent actions shall be maintained.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-710 - Environment and work areas

Current through Register Vol. 40, No. 24, July 15, 2024
Laboratory accommodations, test areas, energy sources,
						lighting, heating and ventilation shall be such as to facilitate proper
						performance of tests. Laboratories shall meet the requirements of subdivisions
						1 through 8 of this section as appropriate to provide compliance with this
						requirement.
1. The environment in
					 which these activities are undertaken shall not invalidate the results or
					 adversely affect the required accuracy of measurement. Particular care shall be
					 taken when such activities are undertaken at sites other than the permanent
					 laboratory premises.
2. The
					 laboratory shall provide for the effective monitoring, control and recording of
					 environmental conditions as appropriate. Such environmental conditions may
					 include biological sterility, dust, electromagnetic interference, humidity,
					 mains voltage, temperature, and sound and vibration levels.
3. In instances where monitoring or control
					 of any of the above-mentioned items are specified in a test method or by
					 regulation, the laboratory shall meet and document adherence to the laboratory
					 facility requirements.
4. There
					 shall be effective separation between neighboring areas in which there are
					 incompatible activities including culture handling or incubation areas and
					 volatile organic chemical handling areas. The laboratory shall take measures to
					 prevent cross-contamination.
5.
					 Access to and use of all areas affecting the quality of these activities shall
					 be defined and controlled.
6.
					 Adequate measures shall be taken to ensure good housekeeping in the laboratory
					 and to ensure that any contamination does not adversely affect data
					 quality.
7. Work spaces shall be
					 available to ensure an unencumbered work area.
8. Work areas include:
a. Access and entryways to the
					 laboratory;
b. Sample receipt
					 areas;
c. Sample storage
					 areas;
d. Chemical and waste
					 storage areas; and
e. Data handling
					 and storage areas.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-720 - Equipment and reference materials

Current through Register Vol. 40, No. 24, July 15, 2024
A. The laboratory shall be furnished with all
					 items of equipment, including reference materials, required for the correct
					 performance of tests for which certification is sought. The laboratory shall
					 maintain records of reference materials sufficient to provide proper
					 performance of tests. In those cases where the laboratory needs to use
					 equipment outside its permanent control it shall ensure that the relevant
					 requirements of this article are met.
B. All equipment shall be properly
					 maintained, inspected and cleaned. Maintenance procedures shall be
					 documented.
C. Any item of the
					 equipment that has been subjected to overloading or mishandling, or that gives
					 suspect results, or has been shown by verification or otherwise to be defective
					 shall be taken out of service immediately, clearly identified as being out of
					 service and, wherever possible, stored at a specified place until it has been
					 repaired and shown by calibration, verification or test to perform
					 satisfactorily. The laboratory shall examine the effect of this defect on
					 previous calibrations or tests.
D.
					 Each item of equipment including reference materials shall be labeled, marked
					 or otherwise identified to indicate its calibration status.
E. Records of each major item of equipment
					 significant to the tests performed shall be maintained. These records shall
					 include documentation on all routine and non-routine maintenance activities.
					 The laboratory shall maintain records of reference materials sufficient to
					 provide proper performance of tests. The records shall include:
1. The name of the item of
					 equipment;
2. The manufacturer's
					 name, type identification, and serial number or other unique
					 identification;
3. Current
					 location, where appropriate;
4.
					 Copy of the manufacturer's instructions, where available;
5. Dates and results of calibrations or
					 verifications or both and date of the next calibration or
					 verification;
6. Details of
					 maintenance carried out to date and planned for the future; and
7. History of any damage, malfunction,
					 modification or repair.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-730 - Test methods and standard operating procedures

Current through Register Vol. 40, No. 24, July 15, 2024
A. Methods documentation.
1. The laboratory shall have documented
					 instructions on the use and operation of all relevant equipment, on the
					 handling and preparation of samples, and for calibration or testing, where the
					 absence of such instructions could jeopardize the calibrations or
					 tests.
2. All instructions,
					 standards, manuals, and reference data relevant to the work of the laboratory
					 shall be maintained up to date and be readily available to the staff.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-740 - Measurement traceability and calibration

Current through Register Vol. 40, No. 24, July 15, 2024
A. General requirements. All equipment used
					 for environmental tests, including equipment for subsidiary measurements (e.g.,
					 for environmental conditions) having a significant effect on the accuracy or
					 validity of the result of the environmental test or sampling shall be
					 calibrated before being put into service and on a continuing basis. The
					 laboratory shall have an established program and procedure for the calibration
					 of its equipment. This includes balances, thermistors, thermometers and control
					 standards. Such a program shall include a system for selecting, using,
					 calibrating, checking, controlling and maintaining measurement standards,
					 reference materials used as measurement standards, and measuring and test
					 equipment used to perform environmental tests.
B. Traceability of calibration.
1. The laboratory shall ensure that the
					 equipment used can provide the uncertainty of measurement needed.
2. The overall program of calibration or
					 verification or both and validation of equipment shall be designed and operated
					 so as to ensure that measurements made by the laboratory are traceable to
					 national standards of measurement.
3. Where traceability of measurements to the
					 International System of Units (SI) is not possible or not relevant, the same
					 requirements for traceability to, for example, certified reference materials,
					 agreed methods or consensus standards, are required. The laboratory shall
					 provide satisfactory evidence of correlation of results, for example by
					 participation in a suitable program of inter-laboratory comparisons,
					 proficiency testing, or independent analysis.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-750 - Quality assurance

Current through Register Vol. 40, No. 24, July 15, 2024
A. General.
					 The laboratory shall have quality control procedures for monitoring the
					 validity of environmental tests undertaken. The resulting data shall be
					 recorded in such a way that trends are detectable and, where practicable,
					 statistical techniques shall be applied to the reviewing of the results. This
					 monitoring shall be planned and reviewed and may include, but not be limited
					 to, the following:
1. Regular use of
					 certified reference materials or internal quality control using secondary
					 reference materials or both.
2.
					 Participation in interlaboratory comparison or proficiency testing
					 program.
3. Replicate tests using
					 the same or different methods.
4.
					 Retesting of retained samples.
5.
					 Correlation of results for different characteristics of a sample (e.g., total
					 phosphate should be greater than or equal to orthophosphate).
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-760 - Quality control requirements

Current through Register Vol. 40, No. 24, July 15, 2024
A.
					 General.
1. The quality control protocols
					 specified by the laboratory's SOPs shall be followed (1VAC30-45-730
					 C). The laboratory shall ensure that either the (i) applicable essential
					 standards outlined in this section through
					 1VAC30-45-775,
					 1VAC30-45-790
					 through
					 1VAC30-45-798,
					 and
					 1VAC30-45-810
					 through 1VAC30-45-818 or(ii) mandated methods or regulations, whichever are
					 more stringent, are incorporated into their method SOPs. When it is not
					 apparent which is more stringent, the quality controls in the mandated method
					 or regulations are to be followed.
2. All quality control measures shall be
					 assessed and evaluated on an ongoing basis and quality control acceptance
					 criteria shall be used to determine the validity of the data. The laboratory
					 shall have procedures for the development of acceptance/rejection criteria
					 where no method or regulatory criteria exists.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-770 - Chemical testing: positive and negative controls

Current through Register Vol. 40, No. 24, July 15, 2024
A. Negative control - method performance.
1. Purpose. The method blank is used to
					 assess the preparation batch for possible contamination during the preparation
					 and processing steps. The method blank shall be processed along with and under
					 the same conditions as the associated samples to include all steps of the
					 analytical procedure. Procedures shall be in place to determine if a method
					 blank is contaminated. Any affected samples associated with a contaminated
					 method blank shall be reprocessed for analysis or the results reported with
					 appropriate data qualifying codes.
2. Frequency. The method blank shall be
					 analyzed at a minimum of one per preparation batch. In those instances for
					 which no separate preparation method is used (e.g., volatiles in water) the
					 batch shall be defined as environmental samples that are analyzed together with
					 the same method and personnel, using the same lots of reagents, not to exceed
					 the analysis of 20 environmental samples.
3. Composition. The method blank shall
					 consist of a quality system matrix that is similar to the associated samples
					 and is known to be free of the analytes of interest.
4. Evaluation criteria and corrective action.
					 While the goal is to have no detectable contaminants, each method blank shall
					 be critically evaluated as to the nature of the interference and the effect on
					 the analysis of each sample within the batch. The source of contamination shall
					 be investigated and measures taken to minimize or eliminate the problem and
					 affected samples reprocessed or data shall be appropriately qualified if:
a. The concentration of a targeted analyte in
					 the blank is at or above the reporting limit as established by the test method
					 or by regulation, and is greater than 1/10 of the amount measured in any
					 sample.
b. The blank contamination
					 otherwise affects the sample results as per the test method requirements or the
					 individual project data quality objectives.
c. When a blank is determined to be
					 contaminated, the cause shall be investigated and measures taken to minimize or
					 eliminate the problem. Samples associated with a contaminated blank shall be
					 evaluated as to the best corrective action for the samples (e.g., reprocessing
					 or data qualifying codes). In all cases the corrective action shall be
					 documented.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-771 - Chemical testing: limit of detection and limit of quantitation

Current through Register Vol. 40, No. 24, July 15, 2024
A. General. All
					 procedures used shall be documented. Documentation shall include the quality
					 system matrix type. All supporting data shall be retained.
B. Limit of detection (LOD). The laboratory
					 shall utilize a test method that provides an LOD that is appropriate and
					 relevant for the intended use of the data. LOD determination and validation are
					 required as specified by 1VAC3045-760 B 1. LODs shall be determined by the
					 protocol in the mandated test method or applicable regulation. If the protocol
					 for determining LODs is not specified, the selection of the procedure shall
					 reflect instrument limitations and the intended application of the test method.
1. The LOD shall be initially determined for
					 the compounds of interest in each test method in a quality system matrix in
					 which there are no target analytes or interferences at a concentration that
					 would impact the results. Alternatively the LOD shall be determined in the
					 quality system matrix of interest (see definition of matrix).
2. LODs shall be determined each time there
					 is a change in the test method that affects how the test is performed, or when
					 a change in instrumentation occurs that affects the sensitivity of the
					 analysis.
3. The LOD shall be
					 verified annually for each quality system matrix, method and analyte according
					 to the procedure as specified in
					 1VAC30-45-760
					 B 1.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-772 - Chemical testing: data reduction

Current through Register Vol. 40, No. 24, July 15, 2024
The procedures for data reduction, such as use of linear
						regression, shall be documented.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-773 - Chemical testing: quality of standards and reagents

Current through Register Vol. 40, No. 24, July 15, 2024
A. The source of standards shall comply with
					 1VAC30-45-740 C.
B. Reagent quality, water quality and checks.
1. Reagents. In methods where the purity of
					 reagents is not specified, analytical reagent grade shall be used. Reagents of
					 lesser purity than those specified by the test method shall not be used. The
					 labels on the container should be checked to verify that the purity of the
					 reagents meets the requirements of the particular test method. Such information
					 shall be documented.
2. Water. The
					 quality of water sources shall be monitored and documented and shall meet
					 method specified requirements.
3.
					 The laboratory will verify the concentration of titrants in accordance with
					 written laboratory procedures.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-774 - Chemical testing: selectivity

Current through Register Vol. 40, No. 24, July 15, 2024
A.
					 The laboratory shall evaluate selectivity by following the checks established
					 within the method, which may include mass spectral tuning, second column
					 confirmation, ICP inter-element interference checks, chromatography retention
					 time windows, sample blanks, spectrochemical absorption or fluorescence
					 profiles, co-precipitation evaluations, and electrode response
					 factors.
B. A confirmation shall be
					 performed to verify the compound identification when positive results are
					 detected on a sample from a location that has not been previously tested by the
					 laboratory. Such confirmations shall be performed on organic tests such as
					 pesticides, herbicides, or acid extractable or when recommended by the
					 analytical test method except when the analysis involves the use of a mass
					 spectrometer. Confirmation is required unless stipulated in writing by the
					 client. All confirmation shall be documented.
C. The laboratory shall document acceptance
					 criteria for mass spectral tuning.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-775 - Chemical testing: constant and consistent test conditions

Current through Register Vol. 40, No. 24, July 15, 2024
A. The laboratory
					 shall assure that the test instruments consistently operate within the
					 specifications required of the application for which the equipment is
					 used.
B. Any cleaning and storage
					 procedures that are not specified by the test method shall be documented in
					 laboratory records and SOPs.
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-780

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-781

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-782

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-783

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-784

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-785

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-786

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-787

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-788

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-790 - Microbiology testing: general

Current through Register Vol. 40, No. 24, July 15, 2024
These standards apply to laboratories undertaking
						microbiological analysis of environmental samples. Microbiological testing
						refers to and includes the detection, isolation, enumeration, or identification
						of microorganisms and/or their metabolites, or determination of the presence or
						absence of growth in materials and media.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-791 - Microbiology testing: sterility checks and blanks, positive and negative controls

Current through Register Vol. 40, No. 24, July 15, 2024
A. Sterility
					 checks and blanks. The laboratory shall demonstrate that the filtration
					 equipment and filters, sample containers, media and reagents have not been
					 contaminated through improper handling or preparation, inadequate
					 sterilization, or environmental exposure.
1. A
					 sterility blank shall be analyzed for each lot of pre-prepared, ready-to-use
					 medium (including chromofluorogenic reagent) and for each batch of medium
					 prepared in the laboratory. This shall be done prior to first use of the
					 medium.
2. For filtration
					 technique, the laboratory shall conduct one beginning and one ending sterility
					 check for each filtration series. The filtration series may include single or
					 multiple filtration units, which have been sterilized prior to beginning the
					 series. For presterilized single use funnels a sterility check shall be
					 performed on one funnel per lot. The filtration series is considered ended when
					 more than 30 minutes elapses between successive filtrations. During a
					 filtration series, filter funnels shall be rinsed with three 20-30 ml portions
					 of sterile rinse water after each sample filtration. In addition, laboratories
					 shall insert a sterility blank after every 10 samples or sanitize filtration
					 units by UV light after each sample filtration.
3. For pour plate technique, sterility blanks
					 of the medium shall be made by pouring, at a minimum, one uninoculated plate
					 for each lot of pre-prepared, ready-to-use media and for each batch of medium
					 prepared in the laboratory.
4.
					 Sterility checks on sample containers shall be performed on at least one
					 container for each lot of purchased, presterilized containers with nonselective
					 growth media. For containers prepared and sterilized in the laboratory, a
					 sterility check shall be performed on one container per sterilized batch with
					 nonselective growth media.
5. A
					 sterility blank shall be performed on each batch of dilution water prepared in
					 the laboratory and on each batch of pre-prepared, ready-to-use dilution water
					 with nonselective growth media.
6.
					 At least one filter from each new lot of membrane filters shall be checked for
					 sterility with nonselective growth media.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-792 - Microbiology testing: test variability and reproducibility

Current through Register Vol. 40, No. 24, July 15, 2024
For test methods that specify colony counts such as
						membrane filter or plated media, duplicate counts shall be performed monthly on
						one positive sample, for each month that the test is performed. If the lab has
						two or more analysts, each analyst shall count typical colonies on the same
						plate. Counts shall be within 10% difference to be acceptable. In a laboratory
						with only one microbiology analyst, the analyst shall count the same plate
						twice, with no more than 5.0% difference between the counts.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-793 - Microbiology testing: method evaluation

Current through Register Vol. 40, No. 24, July 15, 2024
A. Laboratories are required to demonstrate
					 proficiency with the test method prior to first use. This shall be achieved by
					 comparison to a method already approved for use in the laboratory, or by
					 analyzing a minimum of 10 spiked samples whose quality system matrix is
					 representative of those normally submitted to the laboratory, or by analyzing
					 and passing one proficiency test series provided by an approved proficiency
					 sample provider. The laboratory shall maintain this documentation as long as
					 the method is in use and for at least five years past the date of last
					 use.
B. Laboratories shall
					 participate in the proficiency test programs required by Article 3
					 (1VAC30-45-500 et seq.). The results
					 of these analyses shall be used to evaluate the ability of the laboratory to
					 produce acceptable data.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-794 - Microbiology testing: test performance

Current through Register Vol. 40, No. 24, July 15, 2024
A. All growth and recovery media shall be
					 checked to assure that the target organism(s) respond in an acceptable and
					 predictable manner (see
					 1VAC30-45-791 B).
B. To ensure that analysis results are
					 accurate, target organism identity shall be verified as specified in the
					 method, e.g., by use of the completed test, or by use of secondary verification
					 tests such as a catalase test.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-795 - Microbiology testing: data reduction

Current through Register Vol. 40, No. 24, July 15, 2024
The calculations, data reduction and statistical
						interpretations specified by each test method shall be
						followed.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-796 - Microbiology testing: quality of standards, reagents, and media

Current through Register Vol. 40, No. 24, July 15, 2024
A. The laboratory
					 shall ensure that the quality of the reagents and media used is appropriate for
					 the test concerned.
B. Culture
					 media may be prepared from commercial dehydrated powders or may be purchased
					 ready to use. The laboratory may prepare media from basic ingredients when
					 commercial media are not available or when it can be demonstrated that
					 commercial media do not provide adequate results. Media prepared by the
					 laboratory from basic ingredients shall be tested for performance (e.g., for
					 selectivity, sensitivity, sterility, growth promotion, growth inhibition) prior
					 to first use. Detailed testing criteria information shall be defined in either
					 the laboratory's test methods, SOPs, quality manual, or similar
					 documentation.
C. Reagents,
					 commercial dehydrated powders and media shall be used within the shelf-life of
					 the product and shall be documented according to
					 1VAC30-45-730 J.
D. Distilled water, deionized water or
					 reverse osmosis produced water free from bactericidal and inhibitory substances
					 shall be used in the preparation of media, solutions and buffers. The quality
					 of the water shall be monitored for chlorine residual, specific conductance,
					 and heterotrophic bacteria plate count monthly (when in use), when maintenance
					 is performed on the water treatment system, or at startup after a period of
					 disuse longer than one month.
E.
					 Analysis for metals and the Bacteriological Water Quality Test (to determine
					 presence of toxic agents or growth promoting substances) shall be performed
					 annually. Results of these analyses shall meet the specifications of the
					 required method and records of analyses shall be maintained for three years.
					 (An exception to performing the Bacteriological Water Quality Test shall be
					 given to laboratories that can supply documentation to show that their water
					 source meets the criteria, as specified by the method, for Type I or Type II
					 reagent water.)
F. Media, solutions
					 and reagents shall be prepared, used and stored according to a documented
					 procedure following the manufacturer's instructions or the test method.
					 Documentation for media prepared in the laboratory shall include date of
					 preparation, preparer's initials, type and amount of media prepared,
					 manufacturer and lot number, final pH of the media, and expiration date.
					 Documentation for media purchased pre-prepared, ready to use shall include
					 manufacturer, lot number, type of media received, date of receipt, expiration
					 date of the media, and pH of the media.
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-797 - Microbiology testing: selectivity

Current through Register Vol. 40, No. 24, July 15, 2024
In order to ensure identity and traceability, reference
						cultures used for positive and negative controls shall be obtained from a
						recognized national collection, organization, or manufacturer. Microorganisms
						may be single use preparations or cultures maintained by documented procedures
						that demonstrate the continued purity and viability of the organism.
1. Reference cultures may be revived (if
					 freeze-dried) or transferred from slants and subcultured once to provide
					 reference stocks. The reference stocks shall be preserved by a technique that
					 maintains the characteristics of the strains. Reference stocks shall be used to
					 prepare working stocks for routine work. If reference stocks have been thawed,
					 they shall not be refrozen and reused.
2. Working stocks shall not be sequentially
					 cultured more than five times and shall not be subcultured to replace reference
					 stocks.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-800

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-801

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-802

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-803

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-804

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-805

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-806

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-807

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-808

Current through Register Vol. 40, No. 24, July 15, 2024
Statutory Authority: § 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-810 - Air testing: general

Current through Register Vol. 40, No. 24, July 15, 2024
These standards shall apply to samples that are submitted
						to a laboratory for the purpose of analysis. They do not apply to field
						activities such as source air emission measurements or the use of continuous
						analysis devices.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-811 - Air testing: negative and positive controls

Current through Register Vol. 40, No. 24, July 15, 2024
A. Negative controls.
1. Method blanks shall be performed at a
					 frequency of at least one per batch of 20 environmental samples or less per
					 sample preparation method. The results of the method blank analysis shall be
					 used to evaluate the contribution of the laboratory provided sampling media and
					 analytical sample preparation procedures to the amount of analyte found in each
					 sample. If the method blank result is greater than the limit of quantitation
					 and contributes greater than 10% of the total amount of analyte found in the
					 sample, the source of the contamination shall be investigated and measures
					 taken to eliminate the source of contamination. If contamination is found, the
					 data shall be qualified in the report.
2. Collection efficiency. Sampling trains
					 consisting of multiple sections (e.g., filters, sorbent tubes, impingers) that
					 are received intact by the laboratory shall be separated into "front" and
					 "back" sections if required by the client. Each section shall be processed and
					 analyzed separately and the analytical results reported separately.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-812 - Air testing: analytical variability/reproducibility

Current through Register Vol. 40, No. 24, July 15, 2024
Matrix spike duplicates (MSDs) or laboratory duplicates
						shall be analyzed at a minimum of one in 20 samples per sample batch. The
						laboratory shall document their procedure to select the use of appropriate
						types of spikes and duplicates. The selected samples(s) shall be rotated among
						sampling points or sampling locations so that various sample matrix problems
						may be noted and/or addressed. Poor performance in the spikes and duplicates
						may indicate a problem with the sample composition and shall be reported to the
						client.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-813 - Air testing: method evaluation

Current through Register Vol. 40, No. 24, July 15, 2024
In order to ensure the accuracy of the reported result,
						the following procedures shall be in place:
1. Demonstration of capability shall be
					 performed prior to the analysis of any samples and with a significant change in
					 instrument type, personnel, quality system matrix, or test method.
2. Calibration. Calibration protocols
					 specified in
					 1VAC30-45-740 shall be
					 followed.
3. Proficiency test
					 samples. The results of such analyses shall be used by the laboratory to
					 evaluate the ability of the laboratory to produce accurate data.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-814 - Air testing: limit of detection

Current through Register Vol. 40, No. 24, July 15, 2024
The requirements of
						1VAC30-45-771 shall
						apply.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-815 - Air testing: data reduction

Current through Register Vol. 40, No. 24, July 15, 2024
The procedures for data reduction, such as use of linear
						regression, shall be documented.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-816 - Air testing: quality of standards and reagents

Current through Register Vol. 40, No. 24, July 15, 2024
A. The source of standards shall comply with
					 1VAC30-45-740 C.
B. The purity of each analyte standard and
					 each reagent shall be documented by the laboratory through certificates of
					 analyses from the manufacturer/vendor, manufacturer/vendor specifications,
					 and/or independent analysis.
C. In
					 methods where the purity of reagents is not specified, analytical reagent grade
					 or higher quality, if available, shall be used.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.
Virginia Administrative Code › Title 1 - ADMINISTRATION › Agency 30 - DEPARTMENT OF GENERAL SERVICES › Chapter 45 - CERTIFICATION FOR NONCOMMERCIAL ENVIRONMENTAL LABORATORIES › Part II - Standards › Article 4 - Proficiency Testing › Section 1VAC30-45-817 - Air testing: selectivity

Current through Register Vol. 40, No. 24, July 15, 2024
The laboratory shall develop and document acceptance
						criteria for test method selectivity such as absolute and relative retention
						times, wavelength assignments, mass spectral library quality of match, and mass
						spectral tuning.
Statutory Authority
§ 2.2-1105 of the Code of
						  Virginia.