Illinois Administrative Code › Title 35 - ENVIRONMENTAL PROTECTION › Part 611 - PRIMARY DRINKING WATER STANDARDS › Subpart AA - REVISED TOTAL COLIFORM RULE › Section 611.1052 - Analytical Methods and Laboratory Certification

Current through Register Vol. 48, No. 28, July 12, 2024
a) Analytical Methodology
1) The standard sample volume required for
				analysis is 100 mL, regardless of analytical method the supplier
				uses.
2) A supplier needs only
				determine the presence or absence of total coliforms and E. coli; a supplier
				needs not determine density.
3) The
				time from sample collection to initiating test medium incubation may not exceed
				30 hours. Suppliers should but need not hold samples below 10 °C during
				transit.
4) If the supplier is to
				analyze water having residual chlorine (measured as free, combined, or total
				chlorine), the supplier must add sufficient sodium thiosulfate
				(Na2S2O3)
				to the sample bottle before sterilization to neutralize any residual chlorine
				in the water sample. Section 2 of SM 9060 A (97), incorporated by reference in
				Section 611.102, addresses
				dichlorination procedures.
5) The
				supplier must conduct total coliform and E. coli analyses in using certain
				analytical methods, each incorporated by reference in Section 611.102:
BOARD NOTE: The supplier must monitor and analyze only using
				  the version of the approved method in this subsection (a) and incorporated by
				  reference in Section 611.102. The methods listed are the only versions the
				  supplier may use for compliance with this Subpart AA. Laboratories should
				  carefully use only the approved versions of methods, as product package inserts
				  may not be the same as the approved versions of the methods.
A) Total Coliforms, Lactose Fermentation
				Methods
i) Total Coliform Fermentation
				Technique. Sections 1 and 2 of SM 9221 B (94) (only the
				20th ed.), SM 9221 B (99), SM 9221 B (06), or
				sections 1 through 4 of SM 9221 B (14).
BOARD NOTE: The supplier may use commercially available
				  lactose broth in lieu of lauryl tryptose broth if the supplier conducts at
				  least 25 parallel tests between lactose broth and lauryl tryptose broth using
				  the water normally tested and this comparison demonstrates false-positive and
				  false-negative rates for total coliforms are less than ten percent using
				  lactose broth.
ii)
				Presence-Absence (P-A) Coliform Test. Sections 1 and 2 of SM 9221 D (94), SM
				9221 D (99), or sections 1 through 3 of SM 9221 D (14).
BOARD NOTE: A supplier may use a multiple tube enumerative
				  format, as SM 9221 D (94), SM 9221 D (99), or SM 9221 D (14) describes, for
				  presence-absence determination under this Subpart AA.
B) Total Coliforms, Membrane
				Filtration Methods
i) Standard Total Coliform
				Membrane Filter Procedure Using Endo Medium. SM 9222 B (97), SM 9222 B (15), SM
				9222 C (97), or SM 9222 C (15).
ii)
				Membrane Filtration Using MI Medium. USEPA 1604 (02).
iii) Hach 10029 (99)
				(m-ColiBlue24®).
BOARD NOTE: A supplier must begin all filtration series with
				  membrane filtration equipment the supplier sterilized by autoclaving. Exposing
				  filtration equipment to UV light is not adequate to ensure sterilization.
				  Subsequent to the initial autoclaving, the supplier may expose the filtration
				  equipment to UV light to sanitize the funnels between filtrations within a
				  filtration series. Alternatively, the supplier may use
				  manufacturer-pre-sterilized membrane filtration equipment (i.e., disposable
				  funnel units).
iv)
				Chromocult® (00).
v) RAPID'E. coli (20).
BOARD NOTE: A supplier must begin all filtration series with
				  membrane filtration equipment the supplier sterilized by autoclaving. Exposing
				  filtration equipment to UV light is not adequate to ensure sterilization.
				  Subsequent to the initial autoclaving, the supplier may expose the filtration
				  equipment to UV light to sanitize the funnels between filtrations within a
				  filtration series. Alternatively, the supplier may use
				  manufacturer-pre-sterilized membrane filtration equipment (i.e., disposable
				  funnel units).
C) Total Coliforms, Enzyme Substrate Methods
i) Colilert®.
				SM 9223 B (97), SM 9223 B (04), or SM 9223 B (16).
BOARD NOTE: A supplier may use multiple-tube and multi-well
				  enumerative formats for this method in presence-absence determination under
				  this Subpart AA.
ii)
				Colilert®-18. SM 9223 B (97), SM 9223 B (04), or
				SM 9223 B (16).
iii)
				Colisure®. SM 9223 B (97), SM 9223 B (04), or SM
				9223 B (16).
BOARD NOTE: A supplier may use multiple-tube and multi-well
				  enumerative formats for this method in presence-absence determination under
				  this Subpart AA. A supplier may read ColisureTM Test
				  results after an incubation time of 24 hours.
iv) E*Colite®
				(98).
v)
				Readycult® (07).
vi) Modified ColitagT (09) or Modified
				ColitagT (20).
vii) Tecta (14) or
				Tecta (17).
D) E. coli
				(following lactose fermentation methods), EC-MUG Medium. Section 1 of SM 9221 F
				(94), section 1 of SM 9221 F (01), section 1 of SM 9221 F (06), or section 1 of
				SM 9221 F (14).
E) E. coli,
				Partition Methods (following membrane filtration methods)
i) EC Broth with MUG (EC-MUG). Section 1.c(2)
				of SM 9222 G (97) or SM 9222 H (15).
BOARD NOTE: The supplier must make certain changes to the EC
				  broth with MUG (EC-MUG) formulation:
				  1.5 g potassium dihydrogen
				  phosphate (KH2PO4) and
				  0.05 g
				  4-methylumbelliferyl-[BETA]-D-glucuronide.
ii) NA-MUG Medium. Section 1.c(1) of SM 9222
				G (97) or SM 9222 I (15).
F) E. coli, Membrane Filtration Methods
i) Membrane Filtration Using MI Medium. USEPA
				1604 (02).
ii) Hach 10029 (99)
				(m-ColiBlue24®).
BOARD NOTE: A supplier must begin all filtration series with
				  membrane filtration equipment the supplier sterilized by autoclaving. Exposing
				  filtration equipment to UV light is not adequate to ensure sterilization.
				  Subsequent to the initial autoclaving, the supplier may expose the filtration
				  equipment to UV light to sanitize the funnels between filtrations within a
				  filtration series. Exposure of filtration equipment to UV light is not adequate
				  to ensure sterilization. Alternatively, the supplier may use
				  manufacturer-pre-sterilized membrane filtration equipment (i.e., disposable
				  funnel units).
iii)
				Chromocult® (00).
iv) RAPID'E. coli (20).
BOARD NOTE: A supplier must begin all filtration series with
				  membrane filtration equipment the supplier sterilized by autoclaving. Exposing
				  filtration equipment to UV light is not adequate to ensure sterilization.
				  Subsequent to the initial autoclaving, the supplier may expose the filtration
				  equipment to UV light to sanitize the funnels between filtrations within a
				  filtration series. Exposure of filtration equipment to UV light is not adequate
				  to ensure sterilization. Alternatively, the supplier may use
				  manufacturer-pre-sterilized membrane filtration equipment (i.e., disposable
				  funnel units).
G) E. coli, Enzyme Substrate Methods
i) Colilert®.
				SM 9223 B (97), SM 9223 B (04), SM 9223 B (16).
BOARD NOTE: Multiple-tube and multi-well enumerative formats
				  for this method are approved for use in presence-absence determination under
				  this Subpart AA.
ii)
				Colilert®-18. SM 9223 B (97), SM 9223 B (04), SM
				9223 B (16).
iii)
				Colisure®. SM 9223 B (97), SM 9223 B (04), SM
				9223 B (16).
BOARD NOTE: A supplier may use multiple-tube and multi-well
				  enumerative formats for this method in presence-absence determination under
				  this Subpart AA. A supplier may read ColisureTM Test
				  results after an incubation time of 24 hours.
iv) E*Colite®
				(98).
v)
				Readycult® (07).
vi) Modified ColitagT (09) or Modified
				ColitagT (20).
vii) Tecta (14) or
				Tecta (17).
H)
				Simultaneous Detection of Total Coliforms and E. coli by Dual Chromogen
				Membrane Filter Procedure (using m-ColiBlue24®
				medium). SM 9222 J (15).