California Code of Regulations › Title 8 - Industrial Relations › Division 1 - Department of Industrial Relations › Chapter 4 - Division of Industrial Safety › Subchapter 7 - General Industry Safety Orders › Group 16 - Control of Hazardous Substances › Article 109 - Hazardous Substances and Processes › Appendix D - Aerosol Transmissible Pathogens - Laboratory (Mandatory)

Current through Register 2024 Notice Reg. No. 28, July 12, 2024
This appendix contains a list of agents that, when
						  reasonably anticipated to be present, require a laboratory to comply with
						  Section 5199 for laboratory operations by
						  performing a risk assessment and establishing a biosafety plan that includes
						  appropriate control measures as identified in the standard.
Adenovirus (in clinical specimens and in cultures or
						  other materials derived from clinical specimens) Arboviruses, unless identified
						  individually elsewhere in this list (large quantities or high concentrations
						  [FN*] of arboviruses for which CDC recommends BSL-2, e.g., dengue virus;
						  potentially infectious clinical materials, infected tissue cultures, animals,
						  or arthropods involving arboviruses for which CDC recommends BSL-3 or higher,
						  e.g., Japanese encephalitis, West Nile virus, Yellow Fever)
Arenaviruses (large quantities or high concentrations
						  of arenaviruses for which CDC recommends BSL-2, e.g., Pichinde virus;
						  potentially infectious clinical materials, infected tissue cultures, animals,
						  or arthropods involving arenaviruses for which CDC recommends BSL-3 or higher,
						  e.g., Flexal virus)
Bacillus anthracis
(activities with
						  high potential for aerosol production [FN**], large quantities or high
						  concentrations, screening environmental samples from
b.
						  anthracis
-- contaminated locations)
Blastomyces dermatitidis
(sporulating
						  mold-form cultures, processing environmental materials known or likely to
						  contain infectious conidia)
Bordetella pertussis
(aerosol
						  generation, or large quantities or high concentrations)
Brucella abortus, B. canis, B. "maris", B.
						  melitensis, B. suis
(cultures, experimental animal studies, products
						  of conception containing or believed to contain pathogenic
Brucella
spp.)
Burkholderia mallei, B. pseudomallei
(potential for aerosol or droplet exposure, handling infected animals, large
						  quantities or high concentrations)
Cercopithecine herpesvirus (see Herpesvirus
						  simiae)
Chlamydia pneumoniae
(activities with
						  high potential for droplet or aerosol production, large quantities or high
						  concentrations)
Chlamydia psittaci
(activities with
						  high potential for droplet or aerosol production, large quantities or high
						  concentrations, non-avian strains, infected caged birds, necropsy of infected
						  birds and diagnostic examination of tissues or cultures known to contain or be
						  potentially infected with
C. psittaci
strains of avian
						  origin)
Chlamydia trachomatis
(activities with
						  high potential for droplet or aerosol production, large quantities or high
						  concentrations, cultures of lymphogranuloma venereum (LGV) serovars, specimens
						  known or likely to contain
C. trachomatis
)
Clostridium botulinum
(activities with
						  high potential for aerosol or droplet production, large quantities or high
						  concentrations)
Coccidioides immitis, C. posadasii
(sporulating cultures, processing environmental materials known or likely to
						  contain infectious arthroconidia, experimental animal studies involving
						  exposure by the intranasal or pulmonary route)
Corynebacterium diphtheriae
Coxiella burnetti
(inoculation,
						  incubation, and harvesting of embryonated eggs or cell cultures; experimental
						  animal studies, animal studies with infected arthropods, necropsy of infected
						  animals, handling infected tissues)
Crimean-Congo haemorrhagic fever virus
Cytomegalovirus, human (viral production, purification,
						  or concentration)
Eastern equine encephalomyelitis virus (EEEV) (clinical
						  materials, infectious cultures, infected animals or arthropods)
Ebola virus
Epstein-Barr virus (viral production, purification, or
						  concentration)
Escherichia coli,
shiga
						  toxin-producing only (aerosol generation or high splash potential)
Flexal virus
Francisella tularensis
(suspect
						  cultures--including preparatory work for automated identification systems,
						  experimental animal studies, necropsy of infected animals, high concentrations
						  of reduced-virulence strains)
Guanarito virus
Haemophilus influenzae,
type b
Hantaviruses (serum or tissue from potentially infected
						  rodents, potentially infected tissues, large quantities or high concentrations,
						  cell cultures, experimental rodent studies)
Helicobacter pylori
(homogenizing or
						  vortexing gastric specimens)
Hemorrhagic fever -- specimens from cases thought to be
						  due to dengue or yellow fever viruses or which originate from areas in which
						  communicable hemorrhagic fever are reasonably anticipated to be present
Hendra virus
Hepatitis B, C, and D viruses (activities with high
						  potential for droplet or aerosol generation, large quantities or high
						  concentrations of infectious materials)
Herpes simplex virus 1 and 2
Herpesvirus simiae (B-virus) (consider for any material
						  suspected to contain virus, mandatory for any material known to contain virus,
						  propagation for diagnosis, cultures)
Histoplasma capsulatum
(sporulating
						  mold-form cultures, propagating environmental materials known or likely to
						  contain infectious conidia)
Human herpesviruses 6A, 6B, 7, and 8 (viral production,
						  purification, or concentration)
Influenza virus, non-contemporary human (H2N2) strains,
						  1918 influenza strain, highly pathogenic avian influenza (HPAI) (large animals
						  infected with 1918 strain and animals infected with HPAI strains in ABSL-3
						  facilities, loose-housed animals infected with HPAI strains in BSL-3-Ag
						  facilities)
Influenza virus, H5N1 -- human, avian
Junin virus
Kyasanur forest disease virus
Lassa fever virus
Legionella pneumophila,
other
						  legionella-like agents (aerosol generation, large quantities or high
						  concentrations)
Lymphocytic choriomeningitis virus (LCMV) (field
						  isolates and clinical materials from human cases, activities with high
						  potential for aerosol generation, large quantities or high concentrations,
						  strains lethal to nonhuman primates, infected transplantable tumors, infected
						  hamsters)
Machupo virus
Marburg virus
Measles virus
Monkeypox virus (experimentally or naturally infected
						  animals)
Mumps virus
Mycobacterium tuberculosis complex (M.
						  africanum, M. bovis, M. caprae, M. microti, M. pinnipedii, M.
						  tuberculosis
(aerosol-generating activities with clinical specimens,
						  cultures, experimental animal studies with infected nonhuman primates)
Mycobacteria
spp. other than those in
						  the
M. tuberculosis
complex and
M. leprae
(aerosol generation)
Mycoplasma pneumoniae
Neisseria gonorrhoeae
(large
						  quantities or high concentrations, consider for aerosol or droplet
						  generation)
Neisseria meningitidis
(activities
						  with high potential for droplet or aerosol production, large quantities or high
						  concentrations)
Nipah virus
Omsk hemorrhagic fever virus
Parvovirus B19
Prions (bovine spongiform encephalopathy prions, only
						  when supported by a risk assessment)
Rabies virus, and related lyssaviruses (activities with
						  high potential for droplet or aerosol production, large quantities or high
						  concentrations)
Retroviruses, including Human and Simian
						  Immunodeficiency viruses (HIV and SIV) (activities with high potential for
						  aerosol or droplet production, large quantities or high concentrations)
Rickettsia prowazekii, Orientia (Rickettsia)
						  tsutsuagmushi, R. typhi (R. mooseri),
Spotted Fever Group agents
(R. akari, R. australis, R. conorii, R. japonicum, R.
						  rickettsii,
and
R. siberica)
(known or potentially
						  infectious materials; inoculation, incubation, and harvesting of embryonated
						  eggs or cell cultures; experimental animal studies with infected
						  arthropods)
Rift valley fever virus (RVFV)
Rubella virus
Sabia virus
Salmonella
spp. other than
S.
						  typhi
(aerosol generation or high splash potential)
Salmonella typhi
(activities with
						  significant potential for aerosol generation, large quantities)
SARS coronavirus (untreated specimens, cell cultures,
						  experimental animal studies)
Shigella
spp. (aerosol generation or
						  high splash potential)
Streptococcus
spp., group A
Tick-borne encephalitis viruses (Central European
						  tick-borne encephalitis, Far Eastern tick-borne encephalitis, Russian spring
						  and summer encephalitis)
Vaccinia virus
Varicella zoster virus
Variola major virus (Smallpox virus)
Variola minor virus (Alastrim)
Venezuelan equine encephalitis virus (VEEV) (clinical
						  materials, infectious cultures, infected animals or arthropods)
West Nile virus (WNV) (dissection of field-collected
						  dead birds, cultures, experimental animal and vector studies)
Western equine encephalitis virus (WEEV) (clinical
						  materials, infectious cultures, infected animals or arthropods)
Yersinia pestis
(antibiotic resistant
						  strains, activities with high potential for droplet or aerosol production,
						  large quantities or high concentrations, infected arthropods, potentially
						  infected animals)[FN*]
'Large quantities or high concentrations' refers to
						  volumes or concentrations considerably in excess of those typically used for
						  identification and typing activities. A risk assessment must be performed to
						  determine if the quantity or concentration to be used carries an increased
						  risk, and would therefore require aerosol control.[FN**]
'activities with high potential for aerosol generation'
						  include centrifugation