Illinois Administrative Code › Title 35 - ENVIRONMENTAL PROTECTION › Part 611 - PRIMARY DRINKING WATER STANDARDS › Subpart L - MICROBIOLOGICAL MONITORING AND ANALYTICAL REQUIREMENTS › Section 611.531 - Analytical Requirements

Current through Register Vol. 48, No. 28, July 12, 2024
A supplier must use the analytical methods in this Section or
				  Agency-approved alternative methods under Section
				  611.480 to demonstrate
				  compliance with only 611.Subpart B. A supplier must measure pH, temperature,
				  turbidity, and RDCs under the supervision of a certified operator. A supplier
				  must conduct measurements for total coliforms, fecal coliforms and HPC using a
				  certified laboratory in one of the categories in Section
				  611.490(a). The
				  supplier must perform analyses using the methods in this Section, each
				  incorporated by reference in Section
				  611.102:
a) Basic Water Parameters and Microbiological
				Quality
1) The supplier must analyze for pH
				and temperature using one of the methods in Section
				611.611; and
2) The supplier must analyze for total
				coliforms, fecal coliforms, heterotrophic bacteria, and turbidity using
				specific methods and analytical test procedures in USEPA Technical Notes,
				incorporated by reference in Section 611.102:
A) Total Coliforms
BOARD NOTE: The time from sample collection to beginning
				  analysis for source (raw) water samples must not exceed eight hours. The
				  supplier should but needs not hold samples below 10° C during
				  transit.
i) Total Coliform Fermentation
				Technique. SM 9221 A (93), SM 9221 A (94), SM 9221 A (99), SM 9221 A (06), SM
				9221 A (14), SM 9221 B (93), SM 9221 B (94), SM 9221 B (99), SM 9221 B (06), SM
				9221 B (14), SM 9221 C (93), SM 9221 C (94), SM 9221 C (99), SM 9221 C (06), or
				SM 9221 C (14).
BOARD NOTE: The supplier may use commercially available
				  lactose broth in lieu of lauryl tryptose broth if the supplier conducts at
				  least 25 parallel tests between this medium and lauryl tryptose broth using the
				  water it normally tests, and this comparison demonstrates that the
				  false-positive rate and false-negative rate for total coliforms is less than
				  ten percent using lactose broth. If the supplier uses inverted tubes to detect
				  gas production, the media should cover these tubes at least one-half to
				  two-thirds after the supplier adds the sample. The supplier needs not run the
				  completed phase on ten percent of all total coliform-positive confirmed
				  tubes.
ii) Total Coliform
				Membrane Filter Technique. SM 9222 A (91), SM 9222 A (94), SM 9222 A (97), SM
				9222 A (06), SM 9222 A (15), SM 9222 B (91), SM 9222 B (94), SM 9222 B (97),
				9222 B (06), SM 9222 B (15), SM 9222 C (91), SM 9222 C (94), SM 9222 C (97), SM
				9222 C (06), or SM 9222 C (15).
iii) ONPG-MUG (also known as Colilert®).
				SM 9223 (92), SM 9223 (94), SM 9223 (97), SM 9223 B (04), or SM 9223 B
				(16).
B) Fecal Coliforms
BOARD NOTE: The time from collecting the sample to beginning
				  analysis of source (raw) water samples must not exceed eight hours. The
				  supplier should but needs not hold samples below 10° C during
				  transit.
i) Fecal Coliform Procedure.
				SM 9221 E (93), SM 9221 E (94), SM 9221 E (99), SM 9221 E (06), or SM 9221 E
				(14).
BOARD NOTE: A-1 broth may be held up to seven days in a
				  tightly closed screwcap tube at 4° C (39° F).
BOARD NOTE: The supplier may hold A-1 broth up to seven days
				  in a tightly closed screwcap tube at 4 °C (39 °F).
ii) Fecal Coliform Membrane Filter Procedure.
				SM 9222 D (91), SM 9222 D (94), SM 9222 D (97), SM 9222 D (06), or SM 9222 D
				(15).
C) Heterotrophic
				Bacteria
i) Pour Plate Method. SM 9215 B
				(88), SM 9215 B (94), SM 9215 B (00), SM 9215 B (04), or SM 9215 B (16).
BOARD NOTE: The time from collecting the sample to beginning
				  analysis must not exceed eight hours. The supplier should but needs not hold
				  samples below 10 °C during transit.
ii) SimPlate (00).
D) Turbidity
BOARD NOTE: Styrene divinyl benzene beads (e.g., AMCO-AEPA-1
				  or equivalent) and stabilized formazin (e.g., Hach StablCalT or equivalent) are
				  acceptable substitutes for formazin.
i)
				Nephelometric Method. SM 2130 B (88), SM 2130 B (94), SM 2130 B (01); USEPA
				180.1 (93); or
				Hach 8195 (18).
ii) GLI Method 2
				(92).
iii) Laser Nephelometry. Hach
				10133 (00) (FilterTrak).
iv) Laser
				Nephelometry (On-Line). Lovibond PTV 6000 (16), Mitchell M5271 (09), or
				Mitchell M5331 (16).
v) Laser
				Nephelometry (Portable). Lovibond TB 6000 (21).
vi) LED Nephelometry (On-Line). AMI Turbiwell
				(09), Lovibond PTV 1000 (16), Lovibond PTV 2000 (16), Mitchell M5331 (09), or
				Mitchell M5331 (16).
vii) LED
				Nephelometry (Portable). Orion AQ4500 (09), Lovibond TB 3500 (21), Lovibond TB
				5000 (21).
viii) 360°
				Nephelometry. Hach 10258 (16) or Hach 10258
				(18).