Hawaii Administrative Rules › Title 11 - DEPARTMENT OF HEALTH › Subtitle 1 - GENERAL DEPARTMENTAL PROVISIONS › Chapter 20 - RULES RELATING TO PUBLIC WATER SYSTEMS › Appendix K - ANALYTICAL METHODS FOR CONDUCTING TOTAL COLIFORM AND E. COLI ANALYSES (HAR Section 11-20-9.1 (b))

Current through June, 2024
Organism
Methodology Category
Method1
Citation1
Total Coliforms
Lactose
Fermentation
Methods
Standard Total Coliform Fermentation Technique
Standard Methods 9221 B.1, B.2
							 (20th ed.; 21st ed.) 
							 2, 3
Standard Methods Online 9221 B.1, B.2-99 
							 2,3
Presence-Absence (P-A) Coliform Test
Standard Methods 9221 D.1, D.2
							 (20th ed.; 21st ed.) 
							 2, 7
Standard Methods Online 9221 D.1, D.2-99 
							 2, 7
Membrane
Filtration
Methods
Standard Total Coliform Membrane Filter
							 Procedure
Standard Methods 9222 B, C
							 (20th ed.; 21st ed.) 
							 2, 7
Standard Methods Online 9222 B-97 
							 2, 4, 9222 C-97 
							 2, 4
Membrane Filtration using MI medium
EPA Method 1604 
							 2
m-ColiBlue24® Test 
							 2, 4
Chroraocult 
							 2, 4
Enzyme
Substrate
Methods
Colilert®
Standard Methods 9223 B
							 (20th ed.; 21st ed.) 
							 2, 5
Standard Methods Online 9223 B-97 
							 2, 5
Colisure®
Standard Methods 9223 B
							 (20th ed.; 21st ed.) 
							 2, 5, 6
Standard Methods Online 9223 B-97 
							 2, 5, 6
E*Colite® Test 
							 2
Readycult® Test 
							 2
modified Colitag® Test 
							 2
Escherichia coli
Escherichia
coli
Procedure (following Lactose Fermentation
							 Methods)
EC-MUG medium
Standard Methods 9221 F.1
							 (20th ed.; 21st ed.) 
							 2
Escherichia
coli
Partition Method
EC broth with MUG (EC-MUG)
Standard Methods 9222
							 G.1
c
(2)(20th ed.;
							 21st ed.) 
							 2, 8
NA-MUG medium
Standard Methods 9222 G.1
c
(1) (20th
							 ed.; 21st ed.) 
							 2
Membrane Filtration Methods
Membrane Filtration using MI medium
EPA Method 1604 
							 2
m-ColiBlue24® Test 
							 2, 4
Chromocult 
							 2, 4
Enzyme Substrate Methods
Colilert®
Standard Methods 9223 B
							 (20th ed.; 21st ed.) 
							 2, 5
Standard Methods Online 9223 B-97 
							 2, 5, 6
Colisure®
Standard Methods 9223 B
							 (20th ed.; 21st ed.) 
							 2, 5, 6
Standard Methods Online 9223 B-97 
							 2, 5, 6
E*Colite® Test 
							 2
Readycult® Test 
							 2
modified Colitag® Test 
							 2
(a) Footnotes to Appendix K
1 The procedures must be done in
				  accordance with the documents listed in subsection (b) of this Appendix, For
				  Standard Methods, either editions, 20th (1998) or
				  21st (2005), may be used. For the Standard Methods
				  Online, the year in which each method was approved by the Standard Methods
				  Committee is designated by the last two digits following the hyphen in the
				  method number. The methods listed are the only online versions that may be
				  used. For vendor methods, the date of the method listed in subsection (b) of
				  this Appendix is the date/version of the approved method. The methods listed
				  are the only versions that may be used for compliance with this rule.
				  Laboratories should be careful to use only the approved versions of the
				  methods, as product package inserts may not be the same as the approved
				  versions of the methods.
2 Incorporated by reference. See
				  subsection (b) of this Appendix.
3 Lactose broth, as commercially
				  available, may be used in lieu of lauryl tryptose broths if the system conducts
				  at least 25 parallel tests between lactose broth and lauryl tryptose broth
				  using the water normally tested, and if the findings from this comparison
				  demonstrate that the false-positive rate and false-negative rate for total
				  coliforms, using lactose broth, is less than 10 percent.
4 All filtration series must begin
				  with membrane filtration equipment that has been sterilized by autoclaving.
				  Exposure of filtration equipment to UV light is not adequate to ensure
				  sterilization. Subsequent to the initial autoclaving/ exposure of the
				  filtration equipment to UV light may be used to sanitize the funnels between
				  filtrations within a filtration series. Alternatively, membrane filtration
				  equipment that is pre-sterilized by the manufacturer (i.e., disposable funnel
				  units) may be used.
5 Multiple-tube and multi-well
				  enumerative formats for this method are approved for use in presence-absence
				  determination under this regulation.
6 Colisure® results may be
				  read after an incubation time of 24 hours.
7 A multiple tube enumerative
				  format, as described in
Standard Methods for the EKamination of Water
				  and Wastewater
9221, is approved for this method for use in
				  presence-absence determination under this regulation.
8 The following changes must be
				  made to the EC broth with MUG (EC-MUG) formulation: Potassium dihydrogen
				  phosphate, KH2PO4, must be 1.5g,
				  and 4-methylumbelliferyl-Beta-D-glucuronide must be 0.05 g.
(b) Incorporation by reference. The standards required in
				  § 11-20-9.1 are incorporated by reference into this section with the
				  approval of the Director of the Federal Register under
				  5
				  U.S.C, 552(a) and
				  1 CFR part 51. To
				  enforce any edition other than that specified in this section, EPA must publish
				  notice of change in the
Federal Register
and the material must
				  be available to the public. All approved material is available for inspection
				  either electronically at
www.regulations.gov
, in hard copy at the
				  Water Docket, or from the sources indicated below. The Docket ID is
				  EPA-HQ-OW-2008-0878. Hard copies of these documents may be viewed at the Water
				  Docket in the EPA Docket Center, (EPA/DC) EPA West, Room 3334, 1301
				  Constitution Ave., NW, Washington, DC, The EPA Docket Center Public Reading
				  Room is open from 8:30 a.m. to 4:30 p.m., Monday through Friday, excluding
				  legal holidays. The telephone number for the Public Reading Room is
				  1-202-566-1744, and the telephone number for the Water Docket is
				  1-202-566-2426. Copyrighted materials are only available for viewing in hard
				  copy. These documents are also available for inspection at the National
				  Archives and Records Administration (NARA), For information on the availability
				  of this material at NARA, call 1-202-741-6030 or go to:
http://www.archives.gov/federal_register/code_of_federal_regulations/ibr_
				  locations.html
.
(1) American Public Health Association, 800 I Street, NW,
				  Washington, DC 20001
(i) "Standard Methods for the Examination of Water and
				  Wastewater," 20th edition (1998):
(A) Standard Methods 9221, "Multiple-Tube Fermentation
				  Technique for Members of the Coliform Group," B.1, B.2, "Standard Total
				  Coliform Fermentation Technique."
(B) Standard Methods 9221, "Multiple-Tube Fermentation
				  Technique for Members of the Coliform Group," D.1, D.2, "Presence-Absence (P-A)
				  Coliform Test."
(C) Standard Methods 9222, "Membrane Filter Technique for
				  Members of the Coliform Group," B, "Standard Total Coliform Membrane Filter
				  Procedure."
(D) Standard Methods 9222, "Membrane Filter Technique for
				  Members of the Coliform Group," C, "Delayed-Incubation Total Coliform
				  Procedure."
(E) Standard Methods 9223, "Enzyme Substrate Coliform Test,"
				  B, "Enzyme Substrate Test," Colilert® and Colisure®.
(F) Standard Methods 9221, "Multiple Tube Fermentation
				  Technique for Members of the Coliform Group," F.1,
"Escherichia coli
Procedure: EC-MUG medium."
(G) Standard Methods 9222, "Membrane Filter Technique for
				  Members of the Coliform Group," G.1.
c
(2),
"Escherichia
				  coli
Partition Method: EC broth with MUG (EC-MUG)."
(H) Standard Methods 9222, "Membrane Filter Technique for
				  Members of the Coliform Group," G.1.
c
(1),
"Escherichia
				  coli
Partition Method: NA-MUG medium."
(ii) "Standard Methods for the Examination of Water and
				  Wastewater," 21st edition (2005):
(A) Standard Methods 9221, "Multiple-Tube Fermentation
				  Technique for Members of the Coliform Group," B.1, B.2, "Standard Total
				  Coliform Fermentation Technique."
(B) Standard Methods 9221, "Multiple-Tube Fermentation
				  Technique for Members of the Coliform Group," D.1, D.2, "Presence-Absence (P-A)
				  Coliform Test."
(C) Standard Methods 9222, "Membrane Filter Technique for
				  Members of the Coliform Group," B, "Standard Total Coliform Membrane Filter
				  Procedure."
(D) Standard Methods 9222, "Membrane Filter Technique for
				  Members of the Coliform Group," C, "Delayed-Incubation Total Coliform
				  Procedure."
(E) Standard Methods 9223, "Enzyme Substrate Coliform Test,"
				  B, "Enzyme Substrate Test," Colilert® and Colisure®.
(F) Standard Methods 9221, "Multiple Tube Fermentation
				  Technique for Members of the Coliform Group," F.1,
"Escherichia coli
Procedure: EC-MUG medium."
(G) Standard Methods 9222, "Membrane Filter Technique for
				  Members of the Coliform Group," G.1.
c
(2),
"Escherichia
				  coli
Partition Method: EC broth with MUG (EC-MUG)."
(H) Standard Methods 9222, "Membrane Filter Technique for
				  Members of the Coliform Group," G.1.
c
(1),
"Escherichia
				  coli
Partition Method: NA-MUG medium."
(iii) "Standard Methods Online" available at
http://www.standardmetiiods.org
;
(A) Standard Methods Online 9221, "Multiple-Tube Fermentation
				  Technique for Members of the Coliform Group" (1999), B.1, B.2-99, "Standard
				  Total Coliform Fermentation Technique."
(B) Standard Methods Online 9221, "Multiple-Tube Fermentation
				  Technique for Members of the Coliform Group" (1999), D.1, D.2-99,
				  "Presence-Absence (P-A) Coliform Test."
(C) standard Methods Online 9222, "Membrane Filter Technique
				  for Members of the Coliform Group" (1997), B-97, "Standard Total Coliform
				  Membrane Filter Procedure,"
(D) Standard Methods Online 9222, "Membrane Filter Technique
				  for Members of the Coliform Group" (1997), C-97, "Delayed-Incubation Total
				  Coliform Procedure."
(E) Standard Methods Online 9223, "Enzyme Substrate Coliform
				  Test" (1997), B-97, "Enzyme Substrate Test", Colilert® and
				  Colisure®.
(2) Charm Sciences, Inc., 659 Andover Street, Lawrence, MA
				  01843-1032, telephone 1-800-343-2170:
(i) *Colite® - "Charm E*ColiteT Presence/Absence Test for
				  Detection and Identification of Coliform Bacteria and
Escherichia
				  coli
in Drinking Water," January 9, 1998.
(ii) [Reserved]
(3) CPI International, Inc., 5580 Skylane Blvd., Santa Rosa,
				  CA, 95403, telephone 1-800-878-7654:
(i) modified Colitag®, ATP D05-0035 - "Modified ColitagT
				  Test Method for the Simultaneous Detection of
E. coli
and
				  other Total Coliforms in Water," August 28, 2009.
(ii) [Reserved]
(4) EMD Millipore (a division of Merck KGaA, Darmstadt
				  Germany), 290 Concord Road, Billerica, MA 01821, telephone
				  1-800-645-5476:
(i) Chromocult - "Chromocult® Coliform Agar
				  Presence/Absence Membrane Filter Test Method for Detection and Identification
				  of Coliform Bacteria and
Escherichia coli
for Finished
				  Waters," November 2000, Version 1.0.
(ii) Readycult® - "Readycult® Coliforms 100
				  Presence/Absence Test for Detection and Identification of Coliform Bacteria and
Escherichia coli
in Finished Waters," January 2007, Version
				  1.1.
(5) EPA's Water Resource Center (MC-4100T), 1200 Pennsylvania
				  Avenue, NW, Washington, DC 20460, telephone 1-202-566-1729:
(i) EPA Method 1604, EPA B21-R-02-024 - "EPA Method 1604:
				  Total Coliforms and
Escherichia coli
in Water by Membrane
				  Filtration Using a Simultaneous Detection Technique (MI Medium)," September
				  2002,
http://www.epa.gov/nerlcwww/1604sp02.pdf
.
(ii) [Reserved]
(6) Hach Company, P.O. Box 389, Loveland, CO 80539, telephone
				  1-800-604-3493:
(i) m-ColiBlue24® - "Membrane Filtration Method
				  m-ColiBlue24® Broth," Revision 2, August 17, 1999.
(ii) [Reserved]