California Code of Regulations › Title 3 - Food and Agriculture › Division 4 - Plant Industry › Chapter 4 - Plant Pathology › Subchapter 6 - Plant Disease Control › Section 3701.6 - Inspection and Testing Procedures

Current through Register 2024 Notice Reg. No. 28, July 12, 2024
(a)
				  Except as otherwise provided, inspection and testing activities described in
				  this section shall be made by the Department, or its authorized agents which,
				  for the purposes of testing, include the Citrus Clonal Protection Program, the
				  Central California Tristeza Eradication Agency and the Jerry Dimittman
				  Laboratory. Official samples submitted to a facility that is certified by the
				  United States Department of Agriculture to perform specific test(s) shall be
				  eligible to fulfill those specific testing requirements of this program. All
				  inspection and testing procedures shall be conducted at times determined
				  suitable by the Department.
(b)
				  Upon submittal of laboratory protocols, and Department review and acceptance
				  thereof, agents may be granted authorization for performing any of the specific
				  laboratory tests required by these regulations. Any change(s) in protocol(s)
				  must be submitted to the Department for review and acceptance prior to their
				  use in this program.
(c) The
				  diseases and associated disease agents of concern to this program and approved
				  test methods are listed below in Table I. Additional inspections and tests
				  other than provided in this section may be required by the Department.
Table I: Diseases, Disease Agents of Concern and
					 Approved Test Methods/Indicators
Diseases of
								Concern
Disease Agent(s)
								Viruses
Test/Indicator
								Plant
Infectious Variegation, Leaf rugose, Crinkly leaf
Citrus variegation ilarvirus Citrus leaf
							 rugose ilarvirus Citrus crinkly leaf ilarvirus
Sour orange Etrog Citron
Leaf blotch
Citrus leaf blotch virus
(CLBV)
							 aka
Dweet tangor
Dweet mottle
Dweet mottle virus
(DMV)
Leprosis
Citrus leprosis rhabdovirus
Sweet orange
Psorosis A & B (Ring spot)
Citrus psorosis ophiovirus (CPsV)
Sweet orange Dweet tangor
Satsuma Dwarf
Satsuma dwarf virus
(SDV) group
Satsuma mandarin, Dweet tangor, White sesame
							 (
Sesamum indicum
), ELISA
Tatter leaf-Citrange stunt
Apple stem grooving capillovirus
(ASGV) aka Citrus tatter leaf virus (CTLV)
Rusk citrange/RL Citrus excelsa
Tristeza (Quick decline, Stem pitting, Seedling
							 yellows)
Citrus tristeza closterovirus
(CTV)
Mexican lime, ELISA, Immunoimpression Direct
							 tissue-blot immunoassay
Yellow mosaic
Citrus yellow mosaic badnavirus
Sweet orange, pummelo, ELISA
Viroids
Various citrus growth abnormalities and
							 symptomatologies related to citrus viroids including exocortis and
							 cachexia
Citrus exocortis viroid
(CEVd),
Hop stunt viroid
(HSVd), Citrus variants of HSVd,
Citrus viroid-IIa
(CVd-IIa): Non-cachexia, CVd-IIIb &
							 -IIc: Cachexia,
Citrus bent leaf viroid
(CBLVD) aka
CVd-I, Citrus dwarfing viroid
(CDVd) aka CVd-III,
Citrus bark cracking viroid
(CBCVd) aka CVd-IV,
Citrus
							 viroid V
(CVd-V),
Citrus viroid VI
(CVd-VI) aka
							 CVD-OS
Etrog citron Arizona 861-S1/RL and sPAGE,Imprint
							 Hybridization
Procaryotes
Citrus variegated chlorosis
Xylella fastidiosa
PCR and sequencing
Huanglongbing (Citrus greening)
Candidatus
Liberobacter sp.
Sweet orange PCR Source plant observation
Stubborn
Spiroplasma citri
Culture Sweet orange
Witches' broom
Candidatus
Phytoplasma
							 aurantifolia
Mexican lime, PCR
Unknown
Australian Dieback
Uncharacterized, probable phytoplasma
Sweet orange, grapefruit
Concave gum
Unknown
Dweet tangor Sweet orange
Chlorotic dwarf
Unknown
Sour orange, rough lemon
Cristacortis Impietratura
Unknown
Dweet tangor Sweet orange
Vein enation
Unknown, probably Luteovirus
Mexican lime Sour orange
Impietratura
Unknown
Dweet tangor. Sweet orange
(d)
				  Testing. Tests shall be conducted as described in Table 1 unless the Department
				  approves or requires changes.
(1) Mother
				  trees and mother lines shall be tested as follows:
(A) Field-grown registered mother trees shall
				  be tested by the Department annually for Tristeza and Huanglongbing, at least
				  once every 36 months for viroids and at least once every 72 months for
				  psorosis.
(B) Registered mother
				  trees and mother lines, maintained in a departmentally approved
				  insect-resistant structure meeting the requirements of Section
				  3701.5, shall be tested as follows:
1. For Tristeza and Huanglongbing:
A. Prior to their first use as a propagative
				  source but no later than 72 months from the date of propagation, the trees and
				  mother lines shall be tested for Tristeza and Huanglongbing, and for the next
				  two consecutive years following this initial testing.
B. Thereafter, to be used as propagative
				  sources, the trees and mother lines shall have been tested within the previous
				  three years for Tristeza and within the previous six years for Huanglongbing
				  unless the disease is detected in the State. If Huanglongbing is detected in
				  the State, the trees shall have been tested within the previous 36 months for
				  Huanglongbing.
C. Registered trees
				  not in current use as propagative sources must be tested at least once every
				  six years to be eligible to remain in the program.
2. For Viroids:
A. Beginning January 1, 2013, to be used as
				  propagative sources, the trees and mother lines shall have been tested within
				  the previous three years, with the following exception,
B. Beginning January 1, 2016, mother trees
				  and mother lines maintained in insect-resistant structures within which no
				  trees have been tested positive for viroids shall be tested prior to their
				  first use as a propagative source and at least once every six years
				  thereafter.
3. For
				  psorosis: At least once every six years.
(C) In addition to the above, mother trees or
				  mother lines found to be infected with the citrus variant of the HSVd, citrus
				  viroid IIa (CVd-IIa, Non-cachexia variant) shall be tested to ensure the
				  sequence similarity of the detected CVd-IIa with the Department approved growth
				  modifying Tsn-RNA IIa.