Conversation so far:
What can you tell me about Burgundy in relation to Charles V, Holy Roman Emperor?
In 1506, Charles V, Holy Roman Emperor inherited his father's Burgundian territories, most notably the Low Countries and Franche-Comté.
Are there any other interesting aspects about the Charles V, Holy Roman Emperor article?
As Charles V, Holy Roman Emperor was a minor, his aunt Margaret of Austria acted as regent, as appointed by Emperor Maximilian until 1515.

Question: What else happened after she acted as regent of his inheritance?
Self-contained rewrite: What else happened after Margaret of Austria acted as regent of Charles V, Holy Roman Emperor's inheritance?