Conversation so far:
What happened to Charles V in Burgundy?
In 1506, Charles inherited his father's Burgundian territories, most notably the Low Countries and Franche-Comté.
Which territories were included in Charles V inheritance of Burgundian territories?
Most notably Charles inherited the Low Countries and Franche-Comté.
Did any conflict arise because of Charles V's inherited Burgundian territories?
Charles's aunt Margaret of Austria found herself at war with France over the question of Charles' requirement to pay homage to the French king for Flanders.
Who was she that acted as regent for Charles V until 1515?
Charles's aunt Margaret of Austria.
What was the outcome of Charles V's aunt Margaret of Austria's was with France?
The outcome was that France relinquished its ancient claim on Flanders in 1528.
What other signigicant things happened to Charles V in Burgundy other than Margaret of Austria's war wiith France?
Charles extended the Burgundian territory with the annexation of Tournai, Artois, Utrecht, Groningen, and Guelders.

Question: Did the French ever try and get the territory back for themselves?
Self-contained rewrite: Did the French ever try to get the Flanders territory back for themselves from Charles V?