Conversation so far:
How did Charles V, Holy Roman Emperor die?
In August 1558, Holy Roman Emperor Charles V was taken seriously ill with what was later revealed to be malaria. He died morning the morning of 21 September 1558.
How did Charles V, Holy Roman Emperor catch malaria
During his last days what did Charles V, Holy Roman Emperor do?
Holy Roman Emperor Charles V staged his own funeral after which he rose out of the coffin, and withdrew to his apartment. Such a singular solemnity was calculated to inspire.
Did Charles V, Holy Roman Emperor have any kids?
Holy Roman Emperor Charles V and Isabella of Portugal had seven children, though only three survived to adulthood: Philip II of Spain, Maria, and Joanna.

Question: Did he have any other kids?
Self-contained rewrite: Did Charles V, Holy Roman Emperor, have any other kids besides Philip II of Spain, Maria, and Joanna?