Conversation so far:
What can you tell me about Burgundy in relation to Charles V, Holy Roman Emperor?
In 1506, Charles V, Holy Roman Emperor inherited his father's Burgundian territories, most notably the Low Countries and Franche-Comté.
Are there any other interesting aspects about the Charles V, Holy Roman Emperor article?
As Charles V, Holy Roman Emperor was a minor, his aunt Margaret of Austria acted as regent, as appointed by Emperor Maximilian until 1515.
What else happened after Margaret of Austria acted as regent of Charles V, Holy Roman Emperor's inheritance?
Margaret of Austria soon found herself at war with France over the question of Charles V, Holy Roman Emperor's requirement to pay homage to the French king for Flanders.
What was the outcome of Margaret of Autria's war with France over the question of Charles V, Holy Roman Emperor's requirement to pay homage tot eh French King for Flanders?
The outcome was that France relinquished its ancient claim on Flanders in 1528.

Question: What else happen?
Self-contained rewrite: What else happened to Burgundy and the Lower Countries during Charles V, Holy Roman Emperor's reign, besides France relinquishing its ancient claim on Flanders?