Supporting data for: Global Environmental Genomics Reveals Vast Uncultivated Protist Diversity and Taxonomic Blind Spots
Authors: Romero Gutierrez, Miguel Fernando, Krinos, Arianna I., Maurer-Alcala, Xyrus, Burns, John A., Stepanauskas, Ramunas, Woyke, Tanja, Schulz, Frederik
Type: Dataset
Published: 2026-08-03

<p>File description:</p>
<ul>
<li>18S_cluster.tsv (173.60 MB) - Table with annotations and metrics at the 18S rRNA OTU level at 85% identity.</li>
<li>18S_sequence.tsv (787.57 MB) -  Table with annotations and metrics at the 18S rRNA sequence level.</li>
<li>eukcensus_2025_18S.fna (616.19 MB) - Fasta file of 18S rRNA sequences.</li>
<li>rarefied-diversity.tsv (12.41 MB ) - Sample by 18S rRNA OTU count table rarefied using rrarefy with the R vegan package.</li>
<li>eukcensus_2025_img_metadata.tsv (18.08 MB) - Metadata table for IMG/M derived environmental sequencing samples.</li>
<li>eukcensus_2025_sag_assemblies.tar.gz (146.03 MB) - Assemblies for the Single cell Amplified Genomes.</li>
<li>F1_tree.nwk.txt (56.29 KB) - Tree with environmental representative clusters shown in Figure 1 in newick format.</li>
<li>F3A_tree.nwk.txt (190.81 KB) - Tree with environmental representative clusters and orphan representative sequences shown in Figure 3A in newick format.</li>
<li>F3D_tree.nwk.txt (7.42 KB) - Phylogenomic tree including SAGs in the eukaryotic tree shown in Figure 3D in newick format.</li>
<li>marine-correlations-coeff.tsv (868.62 KB) - Correlation coefficients for 18S rRNA and 16S rRNA OTU pairs in marine environments.</li>
<li>marine-correlations-pvalues.tsv (930.37 KB) - Adjusted p-values for 18S rRNA and 16S rRNA OTU pairs in marine environments.</li>
<li>marine-eukcorrelations-coeff.tsv (582.72 KB) - Correlation coefficients for 18S rRNA OTU pairs in marine environments.</li>
<li>marine-eukcorrelations-pvalues.tsv (620.31 KB) - Adjusted p-values for 18S rRNA OTU pairs in marine environments.</li>
<li>freshw-correlations-coeff.tsv (3.00 MB) - Correlation coefficients for 18S rRNA and 16S rRNA OTU pairs in freshwater environments.</li>
<li>freshw-correlations-pvalues.tsv (3.23 MB) - Adjusted p-values for 18S rRNA and 16S rRNA OTU pairs in freshwater environments.</li>
<li>freshw-eukcorrelations-coeff.tsv (9.15 MB) - Correlation coefficients for 18S rRNA OTU pairs in freshwater environments.</li>
<li>freshw-eukcorrelations-pvalues.tsv (9.56 MB) - Adjusted p-values for 18S rRNA OTU pairs in freshwater environments.</li>
<li>soil-correlations-coeff.tsv (547.88 KB) - Correlation coefficients for 18S rRNA and 16S rRNA OTU pairs in soil environments.</li>
<li>soil-correlations-pvalues.tsv (586.42 KB) - Adjusted p-values for 18S rRNA and 16S rRNA OTU pairs in soil environments.</li>
<li>soil-eukcorrelations-coeff.tsv (146.94 KB) - Correlation coefficients for 18S rRNA OTU pairs in soil environments.</li>
<li>soil-eukcorrelations-pvalues.tsv (157.01 KB) - Adjusted p-values for 18S rRNA OTU pairs in soil environments.</li>
<li>16S_cluster.tsv (38.39 MB) - Table with annotations and metrics at the 16S rRNA OTU level at 85% identity.</li>
<li>16S_sequence.tsv (689.23 MB) -  Table with annotations and metrics at the 16S rRNA sequence level.</li>
<li>eukcensus_2025_16S.fna (1.78 GB) - Fasta file of 18S rRNA sequences.</li>
<li>eukcensus_ssuextract.tar.gz (30.54 MB) - Code for the ssuextract software.</li>
<li>SAG_data_SRA.tsv (10.5 KB) - Information for single cell sorts including SRA ID.</li>
</ul>
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