Ferdinand I, Holy Roman Emperor
Ferdinand I  (10 March 1503 – 25 July 1564) was Holy Roman Emperor from 1556, king of Bohemia and Royal Hungary from 1526, and king of Croatia from 1527 until his death in 1564.
Before his accession, he ruled the Austrian hereditary lands of the Habsburgs in the name of his elder brother, Charles V, Holy Roman Emperor.
Also, he often served as Charles' representative in Germany and developed encouraging relationships with German princes.
The key events during his reign were the contest with the Ottoman Empire, which in the 1520s began a great advance into Central Europe, and the Protestant Reformation, which resulted in several wars of religion.
Ferdinand was able to defend his realm and make it somewhat more cohesive, but he could not conquer the major part of Hungary.
His flexible approach to Imperial problems, mainly religious, finally brought more result than the more confrontational attitude of his brother.
Ferdinand's motto was : "Let justice be done, though the world perish".
Archduke Sigismund, Grand Duke of Tuscany
Archduke Sigismund of Austria, Grand Duke of Tuscany(" Sigismund Otto Maria Josef Gottfried Henrich Erik Leopold Ferdinand Von Habsburg- Lothringen"; born 21 April 1966) is the current head of the Tuscan branch of the House of Habsburg- Lorraine and current claimant to the title Grand Duke of Tuscany.
Joanna of Austria, Grand Duchess of Tuscany
Joanna of Austria (German "Johanna von Österreich", Italian "Giovanna d'Austria")
(24 January 1547 – 11 April 1578) was born an Archduchess of Austria as the youngest daughter of Ferdinand I, Holy Roman Emperor, and Anna of Bohemia and Hungary.
By marriage, she was the Grand Princess of Tuscany and later the Grand Duchess of Tuscany.
One of her daughters was Marie de Medici, second wife of King Henry IV of France.
Archduchess Maria Isabella of Austria
Archduchess Maria Isabella of Austria, Princess of Tuscany( 21 May 1834 – 14 July 1901), was an Archduchess of Austria and Princess of Tuscany by birth and Countess of Trapani by marriage to her uncle Prince Francis, Count of Trapani.
Maria Isabella was born in Florence, Grand Duchy of Tuscany, as the daughter of Leopold II, Grand Duke of Tuscany and his second wife, Princess Maria Antonia of Bourbon- Two Sicilies.
Maria Josepha of Bavaria
Maria Josepha of Bavaria
( Maria Josepha Antonia Walburga Felicitas Regula, 20 March 173928 May 1767) was Holy Roman Empress, Queen of the Romans, Archduchess of Austria, Grand Duchess of Tuscany, etc. by her marriage to Joseph II, Holy Roman Emperor.
By birth, she was a Princess and Duchess of Bavaria as the daughter of Charles VII, Holy Roman Emperor, Elector of Bavaria, and Archduchess Maria Amalia of Austria.
Andrew, Duke of Calabria
Andrew, Duke of Calabria( 30 October 1327 – 18 September 1345) was the first husband of Joanna I of Naples, and a son of Charles I of Hungary and brother of Louis I of Hungary.
Christina of Lorraine
Christina of Lorraine or Christine de Lorraine (16 August 1565 – 19 December 1637) was a member of the House of Lorraine and was the Grand Duchess of Tuscany by marriage.
She served as Regent of Tuscany jointly with her daughter-in-law during the minority of her grandson from 1621 to 1628.
Princess Maria Anna of Saxony (1799–1832)
Marie Anna of Saxony, Grand Duchess of Tuscany( 15 November 1799 – 24 March 1832),( full name: Maria Anna Carolina Josepha Vincentia Xaveria Nepomucena Franziska de Paula Franziska de Chantal Johanna Antonia Elisabeth Cunigunde Gertrud Leopoldina), was a princess of Saxony.
She became Grand Duchess of Tuscany by her marriage to Leopold II, Grand Duke of Tuscany.
Anna de' Medici
Anna de' Medici( 31 December
1569 – 19 February 1584) was the third child of Francesco I de' Medici, Grand Duke of Tuscany, and Joanna of Austria.
She was a member of the famous House of Medici.
Anna Maria Franziska of Saxe-Lauenburg
Anna Maria Franziska of Saxe-Lauenburg (13 June 1672 – 15 October 1741) was the legal Duchess of Saxe-Lauenburg in the eyes of the Holy Roman Emperor, the overlord of Saxe-Lauenburg, from 1689 until 1728; however, because her distant cousin George William, Duke of Brunswick-Lüneburg, conquered the duchy by force in 1689, she exercised no control over the territory, instead living in her manors in Bohemia.
She was Grand Duchess of Tuscany as the wife of the last Medici Grand Duke, Gian Gastone.
Anna Maria Franziska was the elder surviving daughter of Julius Franz, Duke of Saxe-Lauenburg, and Maria Hedwig of the Palatinate-Sulzbach.
She married Philipp Wilhelm August of the Palatinate in 1690, with whom she had her only child, Maria Anna, in 1691.
She was widowed in 1693.
Four years later, she married Gian Gastone de' Medici, a Prince of Tuscany.
With her brother-in-law Ferdinando de' Medici's death in 1713, her husband became Tuscany's heir-apparent.
She became Grand Duchess of Tuscany upon the incumbent ruler's death in 1723.
She was Grand Duchess for fourteen years before being widowed again in 1737.

Question: When did Joanna Of Austria, Grand Duchess Of Tuscany's father die?
Answer: 25 July 1564