Anna de' Medici, Archduchess of Austria
Anna de' Medici( 21 July 1616 – 11 September 1676) was a daughter of Cosimo II de' Medici, Grand Duke of Tuscany and his wife Maria Maddalena of Austria.
A patron of the arts, she married Ferdinand Charles, Archduke of Further Austria in 1646.
They were the parents of Claudia Felicitas of Austria, Holy Roman Empress.
Maria of Austria, Holy Roman Empress
Archduchess Maria of Austria (21 June 1528 – 26 February 1603) was Holy Roman Empress and queen consort of Bohemia and Hungary as the spouse of Maximilian II, Holy Roman Emperor and King of Bohemia and Hungary.
She served as regent of Spain in the absence of her father Emperor Charles V from 1548 until 1551, and in the absence of her brother Philip II, from 1558 to 1561.
Maria Theresa of Naples and Sicily
Maria Theresa of Naples and Sicily (6 June 1772 – 13 April 1807) was the last Holy Roman Empress and the first Empress of Austria by marriage to Francis II, Holy Roman Emperor.
She was the eldest daughter of Ferdinand IV & III of Naples and Sicily (later Ferdinand I of the Two Sicilies) (1751–1825) and Marie Caroline of Austria (1752–1814).
Helena Antonia
Helena Antonia( 1550 – 1595) was a bearded female court dwarf of Maria of Austria, Holy Roman Empress and was a favorite of Margaret of Austria, Queen of Spain, and also a lady- in- waiting for Constance of Austria.
She was born in Liège.
Adelaide of Italy
Adelaide of Italy (93116 December 999 AD) , also called Adelaide of Burgundy, was a Holy Roman Empress by marriage to Holy Roman Emperor Otto the Great; she was crowned as the Holy Roman Empress with him by Pope John XII in Rome on 2 February 962.
She was regent of the Holy Roman Empire as the guardian of her grandson in 991-995.
Anna of Tyrol
Anna of Tyrol (4 October 1585 – 14 December 1618), was by birth Archduchess of Austria and member of the Tyrolese branch of the House of Habsburg and by marriage Holy Roman Empress, German Queen, Queen of Bohemia and Queen of Hungary.
The first crowned Holy Roman Empress since the mid-15th century, she was responsible for the moving of the Imperial court from Prague to Vienna, which became one of the centers of European culture.
A proponent of the Counter-Reformation, she held a great influence over her husband, with whom she founded the Imperial Crypt, which later became the burial place of the Habsburg dynasty.
Archduchess Maria Josepha of Austria
Archduchess Maria Josepha of Austria( Maria Josepha Gabriella Johanna Antonia Anna; 19 March 1751, in Vienna – 15 October 1767).
She was the daughter of Francis I, Holy Roman Emperor( 1708 – 1765) and Maria Theresa of Austria, Holy Roman Empress( 1717–1780).
She died of smallpox at the age of 16 and was buried in the Imperial Crypt, Vienna, Austria.
Elisabeth Christine of Brunswick-Wolfenbüttel
Elisabeth Christine of Brunswick- Wolfenbüttel( 28 August 1691 – 21 December 1750) was Princess of Brunswick- Wolfenbüttel, Holy Roman Empress, German Queen, Queen of Bohemia and Hungary; and Archduchess of Austria by her marriage to Emperor Charles VI.
She was renowned for her delicate beauty and also for being the mother of Empress Maria Theresa.
She was the longest serving Holy Roman Empress.
Archduke Otto of Austria (1865–1906)
Archduke Otto Franz Joseph Karl Ludwig Maria of Austria (21 April 1865 – 1 November 1906) was the second son of Archduke Karl Ludwig of Austria (younger brother of Emperor Franz Joseph I of Austria) and his second wife, Princess Maria Annunciata of Bourbon-Two Sicilies.
He was the father of Charles I of Austria, the last Emperor of Austria.
Charles V, Holy Roman Emperor
Charles V (24 February 1500 – 21 September 1558) was Holy Roman Emperor and Archduke of Austria from 1519, King of Spain (Castile and Aragon, as Charles I) from 1516, and Lord of the Netherlands as titular Duke of Burgundy from 1506.
Head of the rising House of Habsburg during the first half of the 16th century, his dominions in Europe included the Holy Roman Empire extending from Germany to northern Italy with direct rule over the Austrian hereditary lands and the Burgundian Low Countries, and a unified Spain with its southern Italian kingdoms of Naples, Sicily, and Sardinia.
Furthermore, his reign encompassed both the long-lasting Spanish and short-lived German colonizations of the Americas.
The personal union of the European and American territories of Charles V was the first collection of realms labelled "the empire on which the sun never sets".
Born in Flanders to Philip "the Handsome" of the Austrian House of Habsburg (son of Maximilian I, Holy Roman Emperor and Mary of Burgundy) and Joanna "the Mad" of the Spanish House of Trastámara (daughter of Isabella of Castile and Ferdinand II of Aragon), Charles inherited all of his family dominions at a young age due to the premature death of his father and the mental illness of his mother.
After the death of Philip in 1506, he inherited the Burgundian Netherlands originally held by his paternal grandmother Mary.
In 1516, he became co-monarch of Spain with his mother Joanna and as such
he was the first king of Spain to inherit the country as dynastically unified by the Catholic Monarchs, his maternal grandparents.
The Spanish possessions at his accession also included the Castilian West Indies and the Aragonese Kingdoms of Naples, Sicily and Sardinia.
At the death of his paternal grandfather Maximilian in 1519, he inherited Austria and was elected to succeed him as Holy Roman Emperor.
He adopted the Imperial name of "Charles V" as his main title, and styled himself as a new Charlemagne.
Charles V revitalized the medieval concept of the universal monarchy and spent most of his life defending the integrity of the Holy Roman Empire from the Protestant Reformation, the expansion of the Ottoman Empire, and a series of wars with France.
He made 40 journeys, travelling from country to country with no single fixed capital city, and it is estimated that he spent a quarter of his reign on the road.
In order to finance the Imperial wars and maintain his armies of Spanish tercios, Italian condottieri, and German Landsknechte, Charles V relied on the economic productivity of the Habsburg Netherlands (birthplace of capitalism) and the flows of South American precious metals to Spain (his chief source of wealth).
Charles ratified the Spanish conquest of the Aztec and Inca empires by the Spanish "Conquistadores" Hernan Cortes and Francisco Pizarro, the establishment of Klein-Venedig by the German Welser family in search of the legendary El Dorado, and the expedition of Ferdinand Magellan around the globe.
In order to consolidate power in his early reign, Charles suppressed two Spanish insurrections (Comuneros' Revolt and Brotherhoods' Revolt) and two German rebellions (Knights' Revolt and Great Peasants' Revolt).
Crowned King in Germany
, Charles sided with Pope Leo X and declared Martin Luther an outlaw at the Diet of Worms (1521).
The same year Francis I of France, surrounded by the Habsburg possessions, started a conflict in Lombardy that lasted until the Battle of Pavia (1525) led to his temporary imprisonment.
In 1527, the Protestant affair re-emerged as Rome was sacked by mutinous imperial soldiers of Lutheran faith.
After ordering the retreat of the troops from the Papal States, Charles V defended Vienna from the Turks and obtained the coronation as King in Italy by Pope Clement VII.
In 1535, he annexed the vacant Duchy of Milan and captured Tunis.
Nevertheless, the Algiers expedition and the loss of Buda in the early 40s frustrated his anti-Ottoman policies.
Meanwhile, Charles V had come to an agreement with Pope Paul III for the organisation of the Council of Trent (1545).
The refusal of the Lutheran Schmalkaldic League to take part in the Council led to a war, won by Charles V with the imprisonment of the Protestant princes.
However, Henry II of France offered new support to the Lutheran cause and strengthened a close alliance with the sultan Suleiman the Magnificent, the ruler of the Ottoman Empire since 1520.
After thirty-five years of incessant warfare and facing the prospect of an alliance between all of his enemies, Charles V conceded the Peace of Augsburg and abandoned his multi-national project with a series of abdications in 1556 that divided his hereditary and imperial domains between the Spanish Habsburgs headed by his son Philip II of Spain and the Austrian Habsburgs headed by his brother Ferdinand, who was Archduke of Austria in Charles' name since 1521 and the designated successor as emperor since 1531.
The Duchy of Milan and the Habsburg Netherlands were left in personal union to the King of Spain, but remained part of the Holy Roman Empire.
The two Habsburg dynasties remained allied until the extinction of the Spanish line.
In 1557, Charles retired to the Monastery of Yuste in Extremadura and there died a year later.

Question: Where was the place of death of Maria Of Austria, Holy Roman Empress's father?
Answer: Yuste