Francis Stewart, 5th Earl of Bothwell
Francis Stewart, 5th Earl of Bothwell( c. December
1562 – November 1612) was Commendator of Kelso Abbey and Coldingham Priory, a Privy Counsellor and Lord High Admiral of Scotland.
Like his stepfather, Archibald Douglas, Parson of Douglas, he was a notorious conspirator, who died in disgrace.
Francis was the first cousin of King James VI of Scotland( they were both grandsons of James V of Scotland).
Francis's maternal uncle James Hepburn, 4th Earl of Bothwell was the chief suspect in the murder of James VI's father Henry Stuart, Lord Darnley.
Henry Frederick, Prince of Wales
Henry Frederick, Prince of Wales (19 February 1594 – 6 November 1612) was the eldest son of James VI and I, King of England and Scotland, and his wife Anne of Denmark.
His name derives from his grandfathers: Henry Stuart, Lord Darnley, and Frederick II of Denmark.
Prince Henry was widely seen as a bright and promising heir to his father's thrones.
However, at the age of 18, he predeceased his father when he died of typhoid fever.
His younger brother Charles succeeded him as heir apparent to the English, Irish and Scottish thrones.
Charles Stuart, 1st Earl of Lennox
Charles Stuart, 1st (or 5th) Earl of Lennox (April or May 1557 – April 1576), was the fourth son of Matthew Stewart, 4th Earl of Lennox and Margaret Douglas, daughter of Margaret Tudor and granddaughter of King Henry VII of England.
His brother was Henry Stuart, Lord Darnley.
Thomas Crawford of Jordanhill
Captain Thomas Crawford or Thomas Craufurd( 1530 – 1603) of Jordanhill( an estate in the West End of Glasgow, part of which is now a college and hospital near Victoria Park) was a trusted confidant of Henry Stuart, Lord Darnley, husband of Mary, Queen of Scots and a retainer of the Matthew Stewart, 4th Earl of Lennox( Darnley's father).
He famously planned the assault and led a small force of 150 men in 1571 that scaled the cliffs and embattlements to expel the castle garrison loyal to Catholic Queen Mary from Dumbarton Castle.
Six years later, he became Provost of Glasgow, establishing a bursary for a student at the university and saving the cathedral from destruction.
Margaret Douglas
Margaret Douglas, Countess of Lennox( 7 October 1515 – 7 March 1578), was the daughter of the Scottish queen dowager Margaret Tudor and her second husband Archibald Douglas, 6th Earl of Angus.
In her youth she was high in the favour of her uncle, Henry VIII of England, but twice incurred the King's anger, first for her unauthorised engagement to Lord Thomas Howard, who died in the Tower of London in 1537 because of his misalliance with her, and again in 1540 for an affair with Thomas Howard's nephew Sir Charles Howard, the brother of Henry's wife Catherine Howard.
On 6 July 1544, she married Matthew Stewart, 4th Earl of Lennox, one of Scotland's leading noblemen.
Her son Henry Stuart, Lord Darnley, married Mary, Queen of Scots, and was the father of James VI and I.
Matthew Stewart, 4th Earl of Lennox
Matthew Stewart, 4th Earl of Lennox (21 September 15164 September 1571), was the fourth Earl of Lennox, and a leader of the Catholic nobility in Scotland.
He was the son of John Stewart, 3rd Earl of Lennox and Lady Elizabeth Stewart, daughter of John Stewart, 1st Earl of Atholl.
His grandson was King James VI of Scotland and I of England.
John Elder (writer)
John Elder( fl. 1542 – 1565) was a Scottish cartographer and writer.
He was the tutor of Henry Stuart, Lord Darnley in England.
Henry Stuart, Lord Darnley
Henry Stuart (or Stewart), Duke of Albany (7 December 1545 – 10 February 1567), styled as Lord Darnley until 1565, was king consort of Scotland from 1565 until his murder at Kirk o' Field in 1567.
Many contemporary narratives describing his life and death refer to him as Lord Darnley, his title as heir apparent to the Earldom of Lennox, and it is by this appellation that he is now generally known.
He was the second but eldest surviving son of Matthew Stewart, 4th Earl of Lennox, and his wife Lady Margaret Douglas.
Darnley's maternal grandparents were Archibald Douglas, 6th Earl of Angus, and Margaret, daughter of Henry VII of England and widow of James IV of Scotland.
He was a first cousin and the second husband of Mary, Queen of Scots, and was the father of her son James VI of Scotland, who succeeded Elizabeth I of England as James I.
Henry Bryant (naturalist)
Henry Bryant( May 12, 1820 – February 2, 1867) was an American physician and naturalist.
He was the grandfather of Henry Bryant Bigelow.
James Hepburn, 4th Earl of Bothwell
James Hepburn( – 14 April 1578), 1st Duke of Orkney and 4th Earl of Bothwell( better known simply as Lord Bothwell), was a prominent Scottish nobleman.
He was known for his association with, abduction of, and marriage to Mary, Queen of Scots, as her third and final husband.
He was accused of the murder of Mary's second husband, Henry Stuart, Lord Darnley, a charge of which he was acquitted.
His marriage to Mary was controversial and divided the country; when he fled the growing rebellion to Scandinavia he was arrested in Norway and lived the rest of his life imprisoned in Denmark.

Question: Who is the paternal grandfather of Henry Stuart, Lord Darnley?
Answer: John Stewart, 3rd Earl of Lennox