Polly & Grant
Polly& Grant( previously known as The ZM Morning Crew and The Polly and Grant Show) is a weekend radio breakfast show on New Zealand radio network More FM, and an internet- only radio station on Rova.
The show is presented by Pauline Gillespie( Polly) and Grant Kereama.
It was previously produced by Marc Peard, known as" New Hot Guy", and previously aired on ZM and The Hits.
In 2007 the Morning Crew won the Best Metropolitan Music Breakfast Hosts award at the New Zealand Radio Awards.
Polly and Molly
Polly and Molly( born 1997), two ewes, were the first mammals to have been successfully cloned from an adult somatic cell and to be transgenic animals at the same time.
This is not to be confused with Dolly the Sheep, the first animal to be successfully cloned from an adult somatic cell where there was no genetic modification carried out on the adult donor nucleus.
Polly and Molly, like Dolly the Sheep, were cloned at the Roslin Institute in Edinburgh, Scotland.
The creation of Polly and Molly built on the somatic nuclear transfer experiments that led to the cloning of Dolly the Sheep.
The crucial difference was that in creating Polly and Molly, scientists used cells into which a new gene had been inserted.
The gene chosen was a therapeutic protein to demonstrate the potential of such recombinant DNA technology combined with animal cloning.
This could hopefully be used to produce pharmacological and therapeutic proteins to treat human diseases.
The protein in question was the human blood clotting factor IX.
Another difference from Dolly the Sheep was the source cell type of the nucleus that was transferred.
In the case of Dolly the Sheep, the nucleus that was transferred came from mammary gland cells from a 6- year- old ewe; in the case of Polly and Molly, fibroblast cells were used.
Prior to the production of Polly and Molly, the only demonstrated way to make a transgenic animal was by microinjection of DNA into the pronuclei of fertilized oocytes( eggs).
However, only a small proportion of the animals will integrate the injected DNA into their genome.
In the rare cases that they do integrate this new genetic information, the pattern of expression of the injected transgene's protein due to the random integration is very variable.
As the aim of such research is to produce an animal that expresses a particular protein in high levels in, for example, its milk, microinjection is a very costly procedure that does not usually produce the desired animal.
In mice, there is an additional option for genetic transfer that is not available in other animals.
Embryonic stem cells provide a means to transfer new DNA into the germline.
They also allow precise genetic modifications by gene targeting.
Modified embryonic stem cells can be selected in vitro before the experiment moves on further for the production of an animal.
Embryonic stem cells capable of contributing to the germline of livestock species such as sheep have not been isolated.
The production of Dolly the Sheep and also Megan and Morag, the two sheep that led to the production of Dolly, demonstrated that viable sheep can be produced by nuclear transfer from a variety of somatic cell types which have been cultured in vitro.
Polly and Molly represented the further step in which somatic cells were cultured in vitro, just as in the case with the previous sheep.
However, in this case they were transfected with foreign DNA, and the transfected cells which stably integrated this new piece of genetic information were selected.
The nuclei of these somatic cells was then transferred into an empty oocyte, as in the procedure of nuclear transfer, and this was used to produce several transgenic animals.
A cell type PDFF was used.
PDFF5 would produce male animals and were not transduced.
Cell type PDFF2 produced female animals and were transduced.
Of the gestations that occurred, three PDFF2 animals were born, two of which survived birth, 7 LL8 and 7LL12.
These animals were transfected but contained a marker gene" not" the cloned gene of interest.
These were named" Holly" and" Olly".
Two more subsets of female- producing PDFF2 cells, PDFF2- 12 and PDFF2- 13, also produced animals which had the cell of interest together with the marker.
Of these lambs, 7LL12, 7LL15, and 7LL13 were born alive and healthy.
Two of these were named Polly and Molly.
Cliff Sterrett
Clifford Sterrett (December 12, 1883 – December 28, 1964) was an American cartoonist best known as the creator of the comic strip "Polly and Her Pals".
Miss Polly
Miss Polly is a 1941 American comedy film directed by Fred Guiol and written by Eugene Conrad and Edward E. Seabrook.
The film stars ZaSu Pitts, Slim Summerville, Kathleen Howard, Brenda Forbes, Elyse Knox and Richard Clayton.
The film was released on November 14, 1941, by United Artists.
Jamie, Frosty and Frank Show
The Jamie, Frosty, and Frank Show was a radio show which ran from 1994 to 1999 on Denver's Alice 105.9 and from 1998 to 1999 on Los Angeles's Star 98.7.
The show was first hosted by Jamie White, Frosty Stilwell and Frank Kramer.
The show was originally called" Frosty and Frank" with Jamie White reading traffic and weather.
Then the show was renamed to" Frosty, Frank, and Jamie", but audience members mistook" Frosty Frank" as a single person so they renamed the show to" Frosty, Jamie, and Frank".
Shortly before the show was moved from Denver to Los Angeles, under contract negotiations, Jamie White had her name moved to first placement.
Each show ended with Jamie, Frosty, and Frank apologizing to anybody and anything they'd potentially offended during that particular broadcast while Brenda Lee's" I'm Sorry" played in the background.
For much of its run on Alice 105.9, the" Jamie, Frosty, and Frank Show" was the highest- rated radio program in the Denver area.
In 1998, however, the trio was moved to Los Angeles, where they quickly rose to the second- highest- rated English language program in a larger and more competitive market.
Star 98.7's own ratings had been dropping for some time.
The show continued to be simultaneously broadcast on Alice 105.9 during that period.
On September 15, 1999, Frosty and Frank were suddenly and inexplicably removed from the show and replaced with Danny Bonaduce, former child- star.
Fans of Jamie, Frosty, and Frank protested, and Jamie herself referred to the change as" a forced decision".
Frosty and Frank were quieter but obviously displeased, stating," Yes, there is a lot to the story, but we ca n't say much" and" I could be better, but that's a long story, and if I told you, I would n't get paid", respectively.
Bonaduce was fired in 2005.
In 2000, Frosty and Frank joined Heidi Hamilton, a former traffic news anchor they had met at Star 98.7, to form the" Frosty, Heidi& Frank" show, which aired on 97.1 FreeFM and later on 790 KABC( AM) Talk Radio in Los Angeles.
On January 4, 2007, Frosty and Frank invited Danny Bonaduce onto the show and the three of them traded behind- the- scenes stories about the drama that had unfolded at Star 98.7.
All three of her former co-hosts alleged that Jamie was an extremely difficult and demanding person to work with, that she schemed behind the scenes to have her co-workers fired, and that she did n't want to share a toll- free number with the other hosts to take callers.
Bonaduce called her a" Texas Chainsaw Massacre of a personality ... the worst experience of my life" and Frosty and Frank said she was a" witch" and" excruciating."
On July 5, 2005, Jamie White was paired up with Jack Heine and Mike" Stench" Roberts to form the Jamie, Jack, and Stench show on Star 98.7, until January 3, 2007, when all three were released from Star 98.7 with the station focusing more on music and artist driven content.
On June 1, 2012, Star 101.3 in San Francisco announced that Frosty Stilwell would join Sandy Stec in hosting the station's morning show.
Since 2012 the program has been known as" The Heidi& Frank Show" has been broadcasting on 95.5 KLOS in Los Angeles.
On September 6th, 2016, Frosty rejoined the Heidi and Frank show on KLOS 95.5 to reunite the Frosty, Heidi and Frank show.
Danetre and Southbrook Learning Village
Danetre and Southbrook Learning Village( DSLV) is an academy school and sixth form for pupils aged 3 to 19, located in Daventry, Northamptonshire, England.
DSLV was formed from the merger of Danetre School, Southbrook Infant and Nursery School and Southbrook Junior School in September 2012.
The school is sponsored by E- ACT.
Academy Leadership Team The Headteacher is Mr R Eadon.
The Primary Headteacher is Mrs J Cadd.
The Deputy Headteachers are Miss S Hadlow and Mrs B Maughan.
The Assistant Headteachers are Miss K Brewster and Mr M Franzoni.
Miss Polly Rae
Miss Polly Rae( born 8 July 1981) is a British singer, dancer, and Neo-burlesque performer.
A Little Pretty Pocket-Book
A Little Pretty Pocket- Book, intended for the Amusement of Little Master Tommy and Pretty Miss Polly with Two Letters from Jack the Giant Killer is the title of a 1744 children's book by British publisher John Newbery.
Frank & Lola
Frank& Lola is a 2016 American neo- noir erotic thriller film written and directed by Matthew Ross in his directorial debut, and starring Michael Shannon, Imogen Poots, Michael Nyqvist, Justin Long, Emmanuelle Devos and Rosanna Arquette.
The film had its world premiere at the 2016 Sundance Film Festival on January 27, 2016.
The film was released theatrically and through video on demand on December 9, 2016, by Universal Pictures and Paladin.
Polly and Her Pals
Polly and Her Pals is an American comic strip created by cartoonist Cliff Sterrett, which ran from December 4, 1912 until December 7, 1958.
It is regarded as one of the most graphically innovative strips of the 20th century.
It debuted as" Positive Polly" on December 4, 1912, in William Randolph Hearst's newspapers, initially the" New York Journal", and was later distributed by King Features Syndicate.
The title changed to" Polly and Her Pals" on January 17, 1913.

Question: Are Miss Polly and Frank & Lola from the same country?