user: List the Pixar movies that were nominated for Academy Awards but did not win.

assistant: <think>
Pixar has produced 26 feature films, starting with Toy Story (1995), which is the first fully computer-animated feature film; its most recent film was Lightyear (2022). The studio has also produced many short films. As of July 2019, its feature films have earned approximately $14 billion at the worldwide box office, with an average worldwide gross of $680 million per film. Toy Story 3 (2010), Finding Dory (2016), Incredibles 2 (2018), and Toy Story 4 (2019) are all among the 50 highest-grossing films of all time. Incredibles 2 is the studio's highest grossing film as well as the fourth-highest-grossing animated film of all time, with a gross of $1.2 billion; the other three also grossed over $1 billion. Moreover, 15 of Pixar's films are in the 50 highest-grossing animated films of all time.
Pixar has earned 23 Academy Awards, 10 Golden Globe Awards, and 11 Grammy Awards, along with numerous other awards and acknowledgments. Its films are frequently nominated for the Academy Award for Best Animated Feature, since its inauguration in 2001, with eleven winners being Finding Nemo (2003), The Incredibles (2004), Ratatouille (2007), WALL-E (2008), Up (2009), Toy Story 3 (2010), Brave (2012), Inside Out (2015), Coco (2017), Toy Story 4 (2019), and Soul (2020). The six nominated films that did not win are Monsters, Inc. (2001), Cars (2006), Incredibles 2 (2018), Onward (2020), Luca (2021) and Turning Red (2022). While Cars 2 (2011), Monsters University (2013), The Good Dinosaur (2015), Finding Dory (2016), Cars 3 (2017) and the aforementioned Lightyear were not nominated. In addition, Toy Story 3 and Up were nominated for the Academy Award for Best Picture.
On February 10, 2009, Pixar executives John Lasseter, Brad Bird, Pete Docter, Andrew Stanton, and Lee Unkrich were presented with the Golden Lion award for Lifetime Achievement by the Venice Film Festival. The physical award was ceremoniously handed to Lucasfilm's founder, George Lucas.</think>
Monsters, Inc. (2001), Cars (2006), Incredibles 2 (2018), Onward (2020), Luca (2021), Turning Red (2022)

user: What is pleitropy?

assistant: Pleiotropy occurs when one gene influences two or more seemingly unrelated phenotypic traits. Such a gene that exhibits multiple phenotypic expression is called a pleiotropic gene. Mutation in a pleiotropic gene may have an effect on several traits simultaneously, due to the gene coding for a product used by a myriad of cells or different targets that have the same signaling function.

Pleiotropy can arise from several distinct but potentially overlapping mechanisms, such as gene pleiotropy, developmental pleiotropy, and selectional pleiotropy. Gene pleiotropy occurs when a gene product interacts with multiple other proteins or catalyzes multiple reactions. Developmental pleiotropy occurs when mutations have multiple effects on the resulting phenotype. Selectional pleiotropy occurs when the resulting phenotype has many effects on fitness (depending on factors such as age and gender).

An example of pleiotropy is phenylketonuria, an inherited disorder that affects the level of phenylalanine, an amino acid that can be obtained from food, in the human body. Phenylketonuria causes this amino acid to increase in amount in the body, which can be very dangerous. The disease is caused by a defect in a single gene on chromosome 12 that codes for enzyme phenylalanine hydroxylase, that affects multiple systems, such as the nervous and integumentary system.

Pleiotropic gene action can limit the rate of multivariate evolution when natural selection, sexual selection or artificial selection on one trait favors one allele, while selection on other traits favors a different allele. Some gene evolution is harmful to an organism. Genetic correlations and responses to selection most often exemplify pleiotropy.

user: Extract and list the names of characters a player can control in The Last of Us mentioned in the paragraph. Also describe relationships between those characters.

assistant: <think>
The Last of Us is an action-adventure game played from a third-person perspective. The player traverses post-apocalyptic environments such as towns, buildings, forests, and sewers to advance the story. The player can use firearms, improvised weapons, hand-to-hand combat, and stealth to defend against hostile humans and cannibalistic creatures infected by a mutated strain of the Cordyceps fungus. For most of the game, the player takes control of Joel, a man tasked with escorting a young girl, Ellie, across the United States. The player also controls Ellie throughout the game's winter segment and briefly controls Joel's daughter, Sarah, in the opening sequence.</think>
Joel: The man who is controlled by the player most of the game.
Ellie: A young girl who is being escorted by Joel across a post-apocalyptic United States.
Sarah: Joel's daughter.