user: What is molecular biology?

assistant: Molecular biology is the branch of biology that seeks to understand the molecular basis of biological activity in and between cells, including biomolecular synthesis, modification, mechanisms, and interactions.[ The study of chemical and physical structure of biological macromolecules is known as molecular biology.

Molecular biology was first described as an approach focused on the underpinnings of biological phenomena - uncovering the structures of biological molecules as well as their interactions, and how these interactions explain observations of classical biology.

In 1945 the term molecular biology was used by physicist William Astbury. In 1953 Francis Crick, James Watson, Rosalind Franklin, and colleagues, working at Medical Research Council unit, Cavendish laboratory, Cambridge (now the MRC Laboratory of Molecular Biology), made a double helix model of DNA which changed the entire research scenario. They proposed the DNA structure based on previous research done by Rosalind Franklin and Maurice Wilkins. This research then lead to finding DNA material in other microorganisms, plants and animals.

Molecular biology is not simply the study of biological molecules and their interactions; rather, it is also a collection of techniques developed since the field's genesis which have enabled scientists to learn about molecular processes. In this way it has both complemented and improved biochemistry and genetics as methods (of understanding nature) that began before its advent. One notable technique which has revolutionized the field is the polymerase chain reaction (PCR), which was developed in 1983. PCR is a reaction which amplifies small quantities of DNA, and it is used in many applications across scientific disciplines.

The central dogma of molecular biology describes the process in which DNA is transcribed into RNA, which is then translated into protein.

Molecular biology also plays a critical role in the understanding of structures, functions, and internal controls within individual cells, all of which can be used to efficiently target new drugs, diagnose disease, and better understand cell physiology. Some clinical research and medical therapies arising from molecular biology are covered under gene therapy whereas the use of molecular biology or molecular cell biology in medicine is now referred to as molecular medicine.

user: How do you install tubeless tires on a bicycle?

assistant: Tubeless tire installs require some setup. It's best to have experience with bicycle tires, and in particular, the use of tire levers to remove and install tires. Make sure your tires and rims are tubeless ready. If the manufacturer also provides a tubeless tape, use that, or use a generic brand. You'll also need sealant and a tubeless stem. The stem might require a tool to unscrew the nozzle for adding sealant. You'll also need a large syringe if you plan on using the stem to add sealant. You'll want a electronic pump, or a manual pump with a reservoir.

I have found that larger tires are easier to install as tubeless but road tires can also be tubeless. The first step is to clean the rim and install tubeless tape around the rim. This should seal the rim component. You'll need to punch a hole for the stem and insert the stem. Next you'll need to lever the tire onto the rim, first one side, then both. If you don't have a syringe, you can add some sealant at this step before fully installing the tire. For road tires, I've found this method prone to spilling.

Next, remove the core / nozzle from the tire stem and hook the stem up to your pump. You want to inject air into the tire quickly and at high pressure to "set" the beads of the tire against the rim. This should result in a popping sound. When you remove the pump, the tire will deflate since you removed the core. If you haven't already, add sealant at this step using a syringe, then reinstall the core and pump up the tire. I usually rotate the tire a bit, then flip it periodically while testing if it holds air. If it does you are done. Happy riding!

user: Why is Utah a great place to visit?

assistant: Utah is a great state to visit especially for nature lovers. There are 5 famous national parks in Utah also known as The Mighty Five: Zion, Bryce Canyon, Arches, Canyonlands and Capitol Reef. Many parks are close to well developed cities which provide great lodging, dining and entertaining options. Park facilities are well maintained. There are numerous recreational activities in the park for all levels and ages and the views are absolutely stunning.