user: Give me a list of beginner friendly Python open source projects to contribute.

assistant: 1. Ansible: IT automation platform for provisioning various resources
2. Jupyter notebook: Interactive notebook popular in data engineering and machine learning world.
3. Pandas: Flexible and powerful data analysis library beloved by data engineers, scientists and analysts.
4. Scrapy: Web crawling and scraping framework written in python.
5. scikit-learn: This library is power packed with implementation of various machine learning algorithms.

user: In one sentence, what is this paragraph conveying?

assistant: <think>
A person's last words, their final articulated words stated prior to death or as death approaches, are often recorded because of the decedent's fame, but sometimes because of interest in the statement itself. (People dying of illness are frequently inarticulate at the end, and in such cases their actual last utterances may not be recorded or considered very important.) Last words may be recorded accurately, or, for a variety of reasons, may not. Reasons can include simple error or deliberate intent. Even if reported wrongly, putative last words can constitute an important part of the perceived historical records or demonstration of cultural attitudes toward death at the time.</think>
The paragraph talk about the finals recorded words a person says at the time of death, and how they could be historically or culturally significance.

user: Given these paragraphs about Multiomics, what is a typical advantage of single-cell multiomics versus bulk analysis?

assistant: <think>
Multiomics, multi-omics, integrative omics, "panomics" or "pan-omics" is a biological analysis approach in which the data sets are multiple "omes", such as the genome, proteome, transcriptome, epigenome, metabolome, and microbiome (i.e., a meta-genome and/or meta-transcriptome, depending upon how it is sequenced); in other words, the use of multiple omics technologies to study life in a concerted way. By combining these "omes", scientists can analyze complex biological big data to find novel associations between biological entities, pinpoint relevant biomarkers and build elaborate markers of disease and physiology. In doing so, multiomics integrates diverse omics data to find a coherently matching geno-pheno-envirotype relationship or association. The OmicTools service lists more than 99 softwares related to multiomic data analysis, as well as more than 99 databases on the topic.

Systems biology approaches are often based upon the use of panomic analysis data. The American Society of Clinical Oncology (ASCO) defines panomics as referring to "the interaction of all biological functions within a cell and with other body functions, combining data collected by targeted tests ... and global assays (such as genome sequencing) with other patient-specific information."

Single-cell multiomics
A branch of the field of multiomics is the analysis of multilevel single-cell data, called single-cell multiomics. This approach gives us an unprecedent resolution to look at multilevel transitions in health and disease at the single cell level. An advantage in relation to bulk analysis is to mitigate confounding factors derived from cell to cell variation, allowing the uncovering of heterogeneous tissue architectures.

Methods for parallel single-cell genomic and transcriptomic analysis can be based on simultaneous amplification or physical separation of RNA and genomic DNA. They allow insights that cannot be gathered solely from transcriptomic analysis, as RNA data do not contain non-coding genomic regions and information regarding copy-number variation, for example. An extension of this methodology is the integration of single-cell transcriptomes to single-cell methylomes, combining single-cell bisulfite sequencing to single cell RNA-Seq. Other techniques to query the epigenome, as single-cell ATAC-Seq and single-cell Hi-C also exist.

A different, but related, challenge is the integration of proteomic and transcriptomic data. One approach to perform such measurement is to physically separate single-cell lysates in two, processing half for RNA, and half for proteins. The protein content of lysates can be measured by proximity extension assays (PEA), for example, which use DNA-barcoded antibodies. A different approach uses a combination of heavy-metal RNA probes and protein antibodies to adapt mass cytometry for multiomic analysis.

Multiomics and machine learning
In parallel to the advances in highthroughput biology, machine learning applications to biomedical data analysis are flourishing. The integration of multi-omics data analysis and machine learning has led to the discovery of new biomarkers. For example, one of the methods of the mixOmics project implements a method based on sparse Partial Least Squares regression for selection of features (putative biomarkers).


https://en.wikipedia.org/wiki/Multiomics</think>
An advantage of single-cell multiomics in relation to bulk analysis is to mitigate confounding factors derived from cell to cell variation, allowing the uncovering of heterogeneous tissue architectures.