user: What kind of cars are good for families with 3 small children?

assistant: When shopping for a car that will accommodate three small children, you need to think about car seats and storage space. Each kid needs to have their own properly installed car seat and you’ll likely need to have enough room for strollers, diaper bags, and other gear. Some mid-size sedans and small SUVs can fit 3 car seats across the back row, but to fit them safely, you’ll need to find the right set of thin car seats the meet the size requirements of your children (e.g. height and weight). For example, Clek makes narrow car seats. That said, taking a trip with 3 kids in these mid-size cars will be challenging unless you add a luggage rack to the top of your car. 

Mid-size SUVs like the Honda Pilot, Kia Telluride, and the Toyota Highlander can accommodate wider car seats in their second row and even include a third row. That said, the third row is difficult to access when car seats are installed in the second row and there isn’t much luggage space when the third row is used for seating.

All that considered, your best bet for 3 kids and lots of gear is a minivan or full size SUV (Chevy Suburban, GMC Yukon XL, Toyota Sequoia). Choosing between those is a matter of preference. It’s best to test drive the various options at local car dealerships.

user: Which are the top 3 states of India that account for receiving the maximum number of interstate emigrants?

assistant: Maharashtra, National Capital of Delhi and Gujarat

user: What is palmitoylation

assistant: Palmitoylation is the covalent attachment of fatty acids, such as palmitic acid, to cysteine (S-palmitoylation) and less frequently to serine and threonine (O-palmitoylation) residues of proteins, which are typically membrane proteins. The precise function of palmitoylation depends on the particular protein being considered. Palmitoylation enhances the hydrophobicity of proteins and contributes to their membrane association. Palmitoylation also appears to play a significant role in subcellular trafficking of proteins between membrane compartments, as well as in modulating protein–protein interactions. In contrast to prenylation and myristoylation, palmitoylation is usually reversible (because the bond between palmitic acid and protein is often a thioester bond). The reverse reaction in mammalian cells is catalyzed by acyl-protein thioesterases (APTs) in the cytosol and palmitoyl protein thioesterases in lysosomes. Because palmitoylation is a dynamic, post-translational process, it is believed to be employed by the cell to alter the subcellular localization, protein–protein interactions, or binding capacities of a protein.

An example of a protein that undergoes palmitoylation is hemagglutinin, a membrane glycoprotein used by influenza to attach to host cell receptors. The palmitoylation cycles of a wide array of enzymes have been characterized in the past few years, including H-Ras, Gsα, the β2-adrenergic receptor, and endothelial nitric oxide synthase (eNOS). In signal transduction via G protein, palmitoylation of the α subunit, prenylation of the γ subunit, and myristoylation is involved in tethering the G protein to the inner surface of the plasma membrane so that the G protein can interact with its receptor.