Chang developed a double-coupling method using an methyl-aza-amino compound to improve on this method, and one alternative to it uses dansyl chloride.
Chang developed a double-coupling method using an methyl-aza-amino compound to improve on this method, and one alternative to it uses dansyl chloride. One version of it uses atomic bombardment and is known as the "subtractive" version. Another alternative to this procedure uses a Curtius rearrangement following an addition by benzyl alcohol, named for Bergmann. It is commonly performed in a buffer of trimethylamine, and combining this technique with HPLC can be used to more effectively determine the identities of the PTH derivatives created. The sequenator automates this process, which begins by reacting the sample with phenyl-isothiocyanate, then cleaving using an acid. For 10 points, name this method of protein sequencing that removes amino acids from the N-terminal tail of a protein.
Chang developed a double-coupling method using an methyl-aza-amino compound to improve on this method, and one alternative to it uses dansyl chloride.
{Edman} degradation