IOriginally, the denaturation phase of this process destroyed the key enzyme, but using a thermostable version from thermophilic bacteria allows denaturation to take place at 110 degrees.
IOriginally, the denaturation phase of this process destroyed the key enzyme, but using a thermostable version from thermophilic bacteria allows denaturation to take place at 110 degrees. During denaturation, all enzymatic activities cease, and its second phase is annealing, during which the primers move around by Bronwnian motion with only the strongest bonds surviving. The primers that survive are thus strongly bonded to the template, allowing the extension phase to take place, during which its namesake enzyme makes copies of the DNA. FTP, identify this this process for copying DNA in vast amounts, invented by Kary Mullis.
IOriginally, the denaturation phase of this process destroyed the key enzyme, but using a thermostable version from thermophilic bacteria allows denaturation to take place at 110 degrees.
Polymerase Chain Reaction