A variation of this technique is used to screen bacterial colonies containing cDNA clones.
A variation of this technique is used to screen bacterial colonies containing cDNA clones. In it, the subject is subjected to electrophoresis on SDS-polyacrylamide gel, then placed on a polymer sheet. A radiolabelled antibody specific to the target protein is then added, binding to the protein and allowing it to be detected by autoradiography. FTP, what is this technique for determining small amounts of a particular protein in tissue samples or cells, named analogously to Southern blotting?
A variation of this technique is used to screen bacterial colonies containing cDNA clones.
{Western} blotting (prompt on protein blotting)