In some experiments, these objects can be supported on a Minusheet perfusion system.
In some experiments, these objects can be supported on a Minusheet perfusion system. In another technique, these objects can be classified into regions of interest demarcated by "gating." Sets of sixteen squares occupying the corners of a three-by-three laser-etched grid can be observed to count these objects, and technologies like FaCS can be used to sort them. Depending upon the density of these objects, and upon whether they are "adherent" or "suspension," there are different ways of (*) "passaging" them. Addition of calcium chloride to these objects allows them to be transfected. Jurkat and HeLa are examples of immortalized "lines" of these objects typically used in culturing them in a laboratory hood. For 10 points, name these objects first discovered by Robert Hooke using microscopy, the basic organizational unit of life. 
In some experiments, these objects can be supported on a Minusheet perfusion system.
cells