Daniel Axelrod invented one variety of this technique which creates an evanescent wave to eliminate background fluorescence, and is known as its TIRF kind.
Daniel Axelrod invented one variety of this technique which creates an evanescent wave to eliminate background fluorescence, and is known as its TIRF kind. It's not spectroscopy, but two photons are shot within a fraction of a femtosecond for the purpose of excitation in one kind of this technique. Osmium tetroxide and aldehydes like glutaraldehyde are common (*) fixing agents in a form of this technique in which one can use a microtome to cut samples. Another form of this technique comes in contact, tapping, and non-contact modes, and employs a tip on a flexible cantilever. Liquid nitrogen is used in the cryo form of this technique which also comes in an atomic force variety, as well as in scanning and transmission types. For 10 points, name this technique that involves shooting a beam of electron to image a sample, 
Daniel Axelrod invented one variety of this technique which creates an evanescent wave to eliminate background fluorescence, and is known as its TIRF kind.
microscopy [accept specifics like atomic force microscopy or electron microscopy; accept word forms like using a microscope; accept total internal reflection fluorescence microscopy or TIRF microscopy before it's mentioned] 