Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

[EX Q]: Facioscapulohumeral muscular dystrophy (FSHD) is a dominant disease linked to contraction of an array of tandem 3.3-kb repeats (D4Z4) at 4q35. Within each repeat unit is a gene, DUX4, that can encode a protein containing two homeodomains. A DUX4 transcript derived from the last repeat unit in a contracted array is associated with pathogenesis but it is unclear how. Using exon-based microarrays, the expression profiles of myogenic precursor cells were determined. Both undifferentiated myoblasts and myoblasts differentiated to myotubes derived from FSHD patients and controls were studied after immunocytochemical verification of the quality of the cultures. To further our understanding of FSHD and normal myogenesis, the expression profiles obtained were compared to those of 19 non-muscle cell types analyzed by identical methods. Many of the ~17,000 examined genes were differentially expressed (>2-fold, p<0.01) in control myoblasts or myotubes vs. non-muscle cells (2185 and 3006, respectively) or in FSHD vs. control myoblasts or myotubes (295 and 797, respectively). Surprisingly, despite the morphologically normal differentiation of FSHD myoblasts to myotubes, most of the disease-related dysregulation was seen as dampening of normal myogenesis-specific expression changes, including in genes for muscle structure, mitochondrial function, stress responses, and signal transduction. Other classes of genes, including those encoding extracellular matrix or pro-inflammatory proteins, were upregulated in FSHD myogenic cells independent of an inverse myogenesis association. Importantly, the disease-linked DUX4 RNA isoform was detected by RT-PCR in FSHD myoblast and myotube preparations only at extremely low levels. Unique insights into myogenesis-specific gene expression were also obtained. For example, all four Argonaute genes involved in RNA-silencing were significantly upregulated during normal (but not FSHD) myogenesis relative to non-muscle cell types. DUX4's pathogenic effect in FSHD may occur transiently at or before the stage of myoblast formation to establish a cascade of gene dysregulation. This contrasts with the current emphasis on toxic effects of experimentally upregulated DUX4 expression at the myoblast or myotube stages. Our model could explain why DUX4's inappropriate expression was barely detectable in myoblasts and myotubes but nonetheless linked to FSHD.
[EX A]: Which disease is associated with the ectopic expression of the protein encoded by the gene DUX4?

[EX Q]: During active transport, molecules move from an area of low concentration to an area of high concentration. This is the opposite of diffusion, and these molecules are said to flow against their concentration gradient. Active transport is called "active" because this type of transport requires energy to move molecules. ATP is the most common source of energy for active transport. As molecules are moving against their concentration gradients, active transport cannot occur without assistance. A carrier protein is always required in this process. Like facilitated diffusion, a protein in the membrane carries the molecules across the membrane, except this protein moves the molecules from a low concentration to a high concentration. These proteins are often called "pumps" because they use energy to pump the molecules across the membrane. There are many cells in your body that use pumps to move molecules. For example, your nerve cells (neurons) would not send messages to your brain unless you had protein pumps moving molecules by active transport. The sodium-potassium pump ( Figure 1.1) is an example of an active transport pump. The sodium-potassium pump uses ATP to move three sodium (Na+ ) ions and two potassium (K+ ) ions to where they are already highly concentrated. Sodium ions move out of the cell, and potassium ions move into the cell. How do these ions then return to their original positions? As the ions now can flow down their concentration gradients, facilitated diffusion returns the ions to their original positions either inside or outside the cell.
[EX A]: complete the following sentence. as molecules are moving against their concentration gradients, active transport cannot occur without __________.

[EX Q]: The movie opens with the Barden Bellas about to perform at the ICCA championships. One of them, Chloe (Brittany Snow) is running late to the start of the performance. When she arrives she is reprimanded by the older Bellas. As they are about to perform, Aubrey (Anna Camp) tries to assure them she'll do a good job, but is shot down. The group sings 'The Sign'.. and all is going well, until the very end when Aubrey has a solo, and projectile vomits all over the stage and front rows of the audience.Cut to the start of a new year at Barden University, and freshman Beca (Anna Kendrick) is arriving by taxi. While she is receiving directions and her Rape Whistle, a car passes her by, and another student, Jesse (Skylar Austin) is singing along to the radio, and catches her attention.Beca finds her new dorm room, and meets her roommate Kimmy-Jin, who is hostile and unfriendly. Beca's dad, a professor at Barden then arrives, and asks her how she got there. It is revealed that Beca's parents have split up as she refers to her dad's new wife as her 'step monster', and seems very reluctant to be attending College. Beca's dad tries to encourage her to enjoy it, but Beca escapes to the Activities fair.In another dorm room, Jesse is meeting his roommate Benji (Ben Platt), who is into Star Wars and magic.At the Activities fair, we are introduced to the Trebles - one of the Campus singing groups, all male, who are talented and have a high opinion of themselves, especially Bumper (Adam Devine) the lead singer. Jesse and Benji approach the Trebles, as Benji is desperate to join, but Bumper is not impressed with his enthusiasm.Across the campus, the Bellas are now down to just 2 members - Aubrey and Chloe, who are trying to recruit new ones. Due to their disastrous performance at the last Championships, they are not having much luck, and are even ridiculed by 'Baloney Barb' who has tried out for them previously. They are then approached by Fat Amy (Rebel Wilson), an outgoing and quirky student from Tasmania who doesn't hesitate to demonstrate her singing and dancing abilities. They then meet Beca, who quickly rebuffs their offer to audition, saying it is lame, which immediately puts Aubrey offside.Instead of being interested in joining any of the singing groups, Beca has much more of an interest in remixing music on her laptop. She gets a job at the campus radio station, and much to her dismay, so does Jesse.Her dad pays her a visit in her dorm, and gives her an ultimatum - if she makes an effort and joins a group at College , but still doesn't like it by the end of the year, she can quit and follow her true dream of becoming a DJ, and he will help her... but he wants her to really make an effort.As Beca goes into the communal showers singing to herself, Chloe happens to be in the showers as well, and confronts Beca in her shower stall , insisting that she try out for the Bellas. Although Beca is mortified, she sings along with Chloe to 'Titanium'.Finally the auditions are on, and Jesse, Benji and Fat Amy are among the hopefuls trying out, auditioning to 'Since you've been gone'. Beca comes late, and hasn't prepared the audition song, but auditions with 'Cups (miss me when I'm gone)', and Chloe and Jesse are impressed, while Aubrey is unsure. We then see the initiation of the new Bellas including Beca, Fat Amy, Stacie (Alexis Knapp), Cynthia-Rose (Esther Dean) and the very softly spoken Lilly (Hana Mae Lee). They all swear an oath and drink the 'blood of the Bellas who came before them' (wine). Cut to the initiation of the new Trebles.. Jesse has made it, but Benji has not. That night, there is Aca-initiation where all the groups
[EX A]:
Who provides an impromptu back-up of one of her favorite songs into the "traditional" song?