Input: Article: In addition to the use of the multiprotein complexes listed above, Gram-negative bacteria possess another method for release of material: the formation of outer membrane vesicles. Portions of the outer membrane pinch off, forming spherical structures made of a lipid bilayer enclosing periplasmic materials. Vesicles from a number of bacterial species have been found to contain virulence factors, some have immunomodulatory effects, and some can directly adhere to and intoxicate host cells. While release of vesicles has been demonstrated as a general response to stress conditions, the process of loading cargo proteins seems to be selective.

Now answer this question: What happens with the pinch off part of the outer membrane?

Output: , forming spherical structures made of a lipid bilayer enclosing periplasmic materials

Article: Downtown New Haven, occupied by nearly 7,000 residents, has a more residential character than most downtowns. The downtown area provides about half of the city's jobs and half of its tax base and in recent years has become filled with dozens of new upscale restaurants, several of which have garnered national praise (such as Ibiza, recognized by Esquire and Wine Spectator magazines as well as the New York Times as the best Spanish food in the country), in addition to shops and thousands of apartments and condominium units which subsequently help overall growth of the city.

Question: What downtown New Haven restaurant was cited by the New York Times, Esquire and Wine Spectator for its outstanding Spanish cuisine?
Ans: Ibiza

Here is a question about this article: In July 1215, with the approbation of Bishop Foulques of Toulouse, Dominic ordered his followers into an institutional life. Its purpose was revolutionary in the pastoral ministry of the Catholic Church. These priests were organized and well trained in religious studies. Dominic needed a frameworka ruleto organize these components. The Rule of St. Augustine was an obvious choice for the Dominican Order, according to Dominic's successor, Jordan of Saxony, because it lent itself to the "salvation of souls through preaching". By this choice, however, the Dominican brothers designated themselves not monks, but canons-regular. They could practice ministry and common life while existing in individual poverty.
What is the answer to this question: Who was the successor to Dominic?
****
So... Jordan of Saxony

The problem: Answer a question about this article:
The U.S. GAO's 2010 findings regarding the great difficulty of accurately gauging the economic impact of copyright infringement was reinforced within the same report by the body's research into three commonly cited estimates that had previously been provided to U.S. agencies. The GAO report explained that the sources a Federal Bureau of Investigation (FBI) estimate, a Customs and Border Protection (CBP) press release and a Motor and Equipment Manufacturers Association estimate "cannot be substantiated or traced back to an underlying data source or methodology."
What did the FBI use as a source?
****
The answer: estimate

Problem: Please answer a question about the following article about Department store:
Selfridges was established in 1909 by American-born Harry Gordon Selfridge on Oxford Street. The company's innovative marketing promoted the radical notion of shopping for pleasure rather than necessity and its techniques were adopted by modern department stores the world over. The store was extensively promoted through paid advertising. The shop floors were structured so that goods could be made more accessible to customers. There were elegant restaurants with modest prices, a library, reading and writing rooms, special reception rooms for French, German, American and "Colonial" customers, a First Aid Room, and a Silence Room, with soft lights, deep chairs, and double-glazing, all intended to keep customers in the store as long as possible. Staff members were taught to be on hand to assist customers, but not too aggressively, and to sell the merchandise. Selfridge attracted shoppers with educational and scientific exhibits; in 1909, Louis Bleriot's monoplane was exhibited at Selfridges (Bleriot was the first to fly over the English Channel), and the first public demonstration of television by John Logie Baird took place in the department store in 1925.
What sort of customers did Selfridges most often cater to?
A: shoppers with educational and scientific exhibits

Question: Read this and answer the question

A brief shoot at London's City Hall was filmed on 18 April 2015, while Mendes was on location. On 17 May 2015 filming took place on the Thames in London. Stunt scenes involving Craig and Seydoux on a speedboat as well as a low flying helicopter near Westminster Bridge were shot at night, with filming temporarily closing both Westminster and Lambeth Bridges. Scenes were also shot on the river near MI6's headquarters at Vauxhall Cross. The crew returned to the river less than a week later to film scenes solely set on Westminster Bridge. The London Fire Brigade was on set to simulate rain as well as monitor smoke used for filming. Craig, Seydoux, and Waltz, as well as Harris and Fiennes, were seen being filmed. Prior to this, scenes involving Fiennes were shot at a restaurant in Covent Garden. Filming then took place in Trafalgar Square. In early June, the crew, as well as Craig, Seydoux, and Waltz, returned to the Thames for a final time to continue filming scenes previously shot on the river.

Who helped the filmakers simulate rain while shooting in London?
Answer:
The London Fire Brigade