In the absence of suitable plate culture techniques, some microbes require culture within live animals. Bacteria such as Mycobacterium leprae and Treponema pallidum can be grown in animals, although serological and microscopic techniques make the use of live animals unnecessary. Viruses are also usually identified using alternatives to growth in culture or animals. Some viruses may be grown in embryonated eggs. Another useful identification method is Xenodiagnosis, or the use of a vector to support the growth of an infectious agent. Chagas disease is the most significant example, because it is difficult to directly demonstrate the presence of the causative agent, Trypanosoma cruzi in a patient, which therefore makes it difficult to definitively make a diagnosis. In this case, xenodiagnosis involves the use of the vector of the Chagas agent T. cruzi, an uninfected triatomine bug, which takes a blood meal from a person suspected of having been infected. The bug is later inspected for growth of T. cruzi within its gut.
If it is possible to answer this question, answer it for me (else, reply "unanswerable"): What is Xenodiagnosis?
Ah, so.. use of a vector to support the growth of an infectious agent

Gaddafi sought to develop closer links in the Maghreb; in January 1974 Libya and Tunisia announced a political union, the Arab Islamic Republic. Although advocated by Gaddafi and Tunisian President Habib Bourguiba, the move was deeply unpopular in Tunisia and soon abandoned. Retaliating, Gaddafi sponsored anti-government militants in Tunisia into the 1980s. Turning his attention to Algeria, in 1975 Libya signed the Hassi Messaoud defence agreement allegedly to counter "Moroccan expansionism", also funding the Polisario Front of Western Sahara in their independence struggle against Morocco. Seeking to diversify Libya's economy, Gaddafi's government began purchasing shares in major European corporations like Fiat as well as buying real estate in Malta and Italy, which would become a valuable source of income during the 1980s oil slump.
If it is possible to answer this question, answer it for me (else, reply "unanswerable"): Who was the president of Tunisia in 1974?
Ah, so.. Habib Bourguiba

By 1820, Charleston's population had grown to 23,000, maintaining its black (and mostly slave) majority. When a massive slave revolt planned by Denmark Vesey, a free black, was revealed in May 1822, whites reacted with intense fear, as they were well aware of the violent retribution of slaves against whites during the Haitian Revolution. Soon after, Vesey was tried and executed, hanged in early July with five slaves. Another 28 slaves were later hanged. Later, the state legislature passed laws requiring individual legislative approval for manumission (the freeing of a slave) and regulating activities of free blacks and slaves.
If it is possible to answer this question, answer it for me (else, reply "unanswerable"): What revolution made blacks fearful of retribution of slaves?
Ah, so..
unanswerable