Problem: Exposure to antibiotics early in life is associated with increased body mass in humans and mouse models. Early life is a critical period for the establishment of the intestinal microbiota and for metabolic development. Mice exposed to subtherapeutic antibiotic treatment (STAT) with either penicillin, vancomycin, penicillin and vancomycin, or chlortetracycline had altered composition of the gut microbiota as well as its metabolic capabilities. Moreover, research have shown that mice given low-dose penicillin (1 g/g body weight) around birth and throughout the weaning process had an increased body mass and fat mass, accelerated growth, and increased hepatic expression of genes involved in adipogenesis, compared to controlled mice. In addition, penicillin in combination with a high-fat diet increased fasting insulin levels in mice. However, it is unclear whether or not antibiotics cause obesity in humans. Studies have found a correlation between early exposure of antibiotics (<6 months) and increased body mass (at 10 and 20 months). Another study found that the type of antibiotic exposure was also significant with the highest risk of being overweight in those given macrolides compared to penicillin and cephalosporin. Therefore, there is correlation between antibiotic exposure in early life and obesity in humans, but whether or not there is a causal relationship remains unclear. Although there is a correlation between antibiotic use in early life and obesity, the effect of antibiotics on obesity in humans needs to be weighed against the beneficial effects of clinically indicated treatment with antibiotics in infancy.
What are some microbiota that can be used for STAT?
---
Answer: unanswerable

Problem: Since the university's establishment in the city in 1837, the histories of the University of Michigan and Ann Arbor have been closely linked. The town became a regional transportation hub in 1839 with the arrival of the Michigan Central Railroad, and a northsouth railway connecting Ann Arbor to Toledo and other markets to the south was established in 1878. Throughout the 1840s and the 1850s settlers continued to come to Ann Arbor. While the earlier settlers were primarily of British ancestry, the newer settlers also consisted of Germans, Irish, and African-Americans. In 1851, Ann Arbor was chartered as a city, though the city showed a drop in population during the Depression of 1873. It was not until the early 1880s that Ann Arbor again saw robust growth, with new immigrants coming from Greece, Italy, Russia, and Poland. Ann Arbor saw increased growth in manufacturing, particularly in milling. Ann Arbor's Jewish community also grew after the turn of the 20th century, and its first and oldest synagogue, Beth Israel Congregation, was established in 1916.
What railroad arrived in 1893?
---
Answer: unanswerable

Problem: Around the sixth century AD, a tribe of Slavs arrived in a portion of Central Europe. According to legend they were led by a hero named Cech, from whom the word "Czech" derives. The ninth century brought the state of Great Moravia, whose first ruler (Rastislav of Moravia) invited Byzantine ruler Michael III to send missionaries in an attempt to reduce the influence of East Francia on religious and political life in his country. These missionaries, Constantine and Methodius, helped to convert the Czechs from traditional Slavic paganism to Christianity and established a church system. They also brought the Glagolitic alphabet to the West Slavs, whose language was previously unwritten. This language, later known as Proto-Czech, was beginning to separate from its fellow West Slavic hatchlings Proto-Slovak, Proto-Polish and Proto-Sorbian. Among other features, Proto-Czech was marked by its ephemeral use of the voiced velar fricative consonant (//) and consistent stress on the first syllable.
When did Rastislav of Moravia arrive in Glagolitic?
---
Answer:
unanswerable