Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.
--------
Question: The French Revolution broke out in 1789. At the time France was in a crisis. The government was badly run and people's lives were miserable. King Louis XIV tried to control the national parliament and raise more taxes. But his effort failed. He ordered his troops to Versailles. The people thought that Louis intended to put down the Revolution by force. On July 14, 1789, they stormed and took the Bastille, where political prisoners were kept. Ever since that day, July 14 has been the French National Day. Louis tried to flee the country in 1792, to get support from Austria and Prussia. However, he was caught and put in prison. In September 1792, the monarchy was abolished _ . In the same year, Louis was executed. A few months later his wife, Marie also had her head cut off. The Revolution of France had frightened the other kings of Europe. Armies from Austria and Prussia began to march against France. The French raised republican armies to defend the nation. The Revolution went through a period of terror. Thousands of people lost their lives. In the end, power passed to Napoleon Bonaparte.

Answer: What's this passage about?

Question: The product of the multiple endocrine neoplasia type 1 (MEN1) tumor suppressor gene, menin, is an integral component of MLL1/MLL2 histone methyltransferase complexes specific for Lys4 of histone H3 (H3K4). We show that menin is a transcriptional coactivator of the nuclear receptors for estrogen and vitamin D. Activation of the endogenous estrogen-responsive TFF1 (pS2) gene results in promoter recruitment of menin and in elevated trimethylation of H3K4. Knockdown of menin reduces both activated TFF1 (pS2) transcription and H3K4 trimethylation. In addition, menin can directly interact with the estrogen receptor-alpha (ERalpha) in a hormone-dependent manner. The majority of disease-related MEN1 mutations prevent menin-ERalpha interaction. Importantly, ERalpha-interacting mutants are also defective in coactivator function. Our results indicate that menin is a critical link between recruitment of histone methyltransferase complexes and nuclear receptor-mediated transcription.

Answer: Which is the histone residue methylated by MLL1?

Question: Restriction endonucleases play a very important role in genetic engineering and DNA mapping. Among hundreds of restriction endonucleases, the Eco R1 enzyme is the most useful and widely investigated enzyme. After sonication and ultracentrifugation, crude extracts of E. coli RY 13 were purified by employing the polyethyleneimine precipitate, ammonium sulfate precipitate and heparin Sepharose-4B affinity column chromatography. The Eco R1 enzyme were purified at about 42 folds and the specific activity was about 100,000 U/mg of protein. The whole purification procedure was finished within two days. The recovery was about 42%. The enzyme was sufficiently concentrated for direct specific DNA hydrolysis.

Answer:
Which bacteria was EcoRI, restriction endonuclease isolated from?