Instructions: In this task, you are given a passage which has a question and the context. You have to generate an answer to the question based on the information present in the context.
Input: Context: Np63, a splice variant of p63, is overexpressed and exhibits oncogenic activity in many cancers including pancreatic and breast cancer and promotes cell survival by inhibiting apoptosis. Despite its role in tumorigenesis, mechanistic activity of Np63 mediated oncogenic function in osteosarcoma is poorly understood.', 'The expression levels of p63 isoforms in osteosarcoma cell lines were identified using quantitative techniques. Expression profiling using microarray, siRNA mediated loss-of-function, and chromatin immunoprecipitation assays were employed to identify novel Np63 targets in p63-null osteosarcoma SaOS-2 cells that were engineered to express Np63. The phenotype of SaOS-2-Np63 cells was assessed using wound-healing, colony formation, and proliferation assays.', 'The comparative expression analyses identified Np63 as the predominant p63 isoform expressed by invasive OS cell lines. Phenotypic analyses of SaOS-2-Np63 cells in vitro indicate that Np63 imparted tumorigenic attributes upon tumor cells. Further, we show that in osteosarcoma cells Np63 directly regulated the transcription factor GLI2, which is a component of the hedgehog signaling pathway, and that functional interactions between Np63 and GLI2 confer oncogenic properties upon OS cells.\Question: Does Np63 enhance the oncogenic phenotype of osteosarcoma cells by inducing the expression of GLI2?
Output:
Here, we report that GLI2 is the novel target gene of Np63 and that Np63-GLI2 crosstalk in osteosarcoma cells is a necessary event in osteosarcoma progression. Defining the exact mechanisms involved in this interaction that mediate the pathogenesis of osteosarcoma promises to identify targets for drug therapy.