You will be given a definition of a task first, then some input of the task.
Given a passage classify if the passage has a definite objective/aim/goal or not. Output '1' if the passage has a defininte objective/aim/goal and output '0' if the passage does not have a definite objective/aim/goal.

Renal magnesium (Mg2+) wasting is one of the ciclosporin (CsA) tubular effects. The major site of Mg2+ transport is the thick ascending limb (TAL), where 70% of the ultrafiltrable Mg2+ is reabsorbed paracellularly. Paracellin-1 is a tight junction protein, which regulates the paracellular Mg2+ transport in the TAL. We hypothesize that CsA reduces the expression and function of paracellin-1 and accounts for the observed renal Mg2+ wasting.', 'We established an immortalized cultured cortical TAL (cTAL) cell line from L-PK/Tag1 transgenic mice by microdissection. The cultured cells expressed paracellin-1 and the characteristics of cTAL cells. Real-time PCR and western blotting were used to test the CsA effects on paracellin-1 expression of cultured cTAL cells. Cytosolic-free Mg2+ concentration [Mg2+]i change with time in a single cTAL cell was used as an indicator of transcellular Mg2+ transport and assessed by using fluorescence dye Mag-fura-2 AM. Paracellular Mg2+ transport was measured by cells grown in porous filters.', 'The results showed that CsA significantly reduced paracellin-1 mRNA and protein expression in a dose-dependent manner. CsA (100 ng/ml) incubation for 24 h induced a decrease of paracellin-1 mRNA by 89.4% and paracellin-1 protein by 75.4%. CsA (100 ng/ml) did not change transcellular Mg2+ transport, but paracellular Mg2+ transport was decreased in CsA-treated cTAL cells by 74.4%.
Output:
0