Definition: In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.
Input: Graphical Abstract Highlights d Virolectins detect and distinguish between closely related O-Ac-Sias in situ d O-Ac-sialoglycans occur in nature in a diversity not appreciated so far d O-Ac-Sias are differentially expressed in a species-, tissue-, and cell-specific fashion d There is extensive cell-to-cell variability in O-Ac-Sia expression in vivo and in vitro In Brief Postsynthetically modified sialic acids serve as critical tags in biomolecular recognition events, both in health and disease. Langereis et al. introduce methodology, based on nidovirus lectins and enzymes, for in situ detection of distinct O-acetylated sialic acid species and demonstrate its applicability by exploring sialoglycan distribution in mammalian cells and tissues. SUMMARY Sialic acids (Sias), 9-carbon-backbone sugars, are among the most complex and versatile molecules of life. As terminal residues of glycans on proteins and lipids, Sias are key elements of glycotopes of both cellular and microbial lectins and thus act as important molecular tags in cell recognition and signaling events. Their functions in such interactions can be regulated by post-synthetic modifications, the most common of which is differential Sia-O-acetylation (O-Ac-Sias). The biology of O-Ac-Sias remains mostly unexplored, largely because of limitations associated with their specific in situ detection. Here, we show that dualfunction hemagglutinin-esterase envelope proteins of nidoviruses distinguish between a variety of closely related O-Ac-Sias. By using soluble forms of hemagglutinin-esterases as lectins and sialate-O-acetylesterases, we demonstrate differential expression of distinct O-Ac-sialoglycan populations in an organ-, tissue-and cell-specific fashion. Our findings indicate that programmed Sia-O-acetylation/de-O-acetylation may be critical to key aspects of cell development, homeostasis, and/or function.
Output:
Complexity and Diversity of the Mammalian Sialome Revealed by Nidovirus Virolectins Cell Reports Resource Complexity and Diversity of the Mammalian Sialome Revealed by Nidovirus Virolectins