In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

[EX Q]: The high mutation rate of the human immunodeficiency virus type 1 (HIV-1) virus is a major problem since it evades the function of antibodies and chemical inhibitors. Here, we demonstrate a viral detection strategy based on synthetic biology principles to detect a specific viral function rather than a particular viral protein. The resistance caused by mutations can be circumvented since the mutations that cause the loss of function also incapacitate the virus. Many pathogens encode proteases that are essential for their replication and that have a defined substrate specificity. A genetically encoded sensor composed of a fused membrane anchor, viral protease target site, and an orthogonal transcriptional activator was engineered into a human cell line. The HIV-1 protease released the transcriptional activator from the membrane, thereby inducing transcription of the selected genes. The device was still strongly activated by clinically relevant protease mutants that are resistant to protease inhibitors. In the future, a similar principle could be applied to detect also other pathogens and functions.
[EX A]: Function-Based Mutation-Resistant Synthetic Signaling Device Activated by HIV-1 Proteolysis

[EX Q]: The present study describes the development of SYBR Green based real-time polymerase chain reaction (real-time PCR) for detection and quantitation of canine parvovirus type 2 (CPV 2) in faecal samples of dogs. In this assay, the primers were designed and custom-synthesized based on nucleotide sequence of VP2 gene of CPV 2. A standard curve was plotted using 10-fold serial dilution of standard plasmid DNA and Ct value. The standard curve was found to be linear over a 10 7 dilution. The real-time PCR results were expressed as the number of DNA copies of CPV 2 per mg of faecal samples and showed range of 1.0 10 3 to 7.0 10 9 copies of viral DNA per mg of stool samples. The analytical sensitivity of the SYBR Green based real-time PCR was shown to be equivalent to 10 copies. Faecal samples (47) from dogs suspected of CPV 2 infection were analyzed by real-time PCR, haemagglutination (HA) assay and by a conventional PCR and 24, 20 and 22 samples were found positive for CPV 2, respectively. Comparison between the results of three different assays revealed that real-time PCR is more sensitive than HA and conventional PCR and allow the detection of low titers of CPV 2 in infected dogs.
[EX A]: Development of a SYBR Green based real-time PCR assay for detection and quantitation of canine parvovirus in faecal samples

[EX Q]: Cut-off Cut-off Supplementary Figure 1 | Setting cut-off to identify links and hidden source. (a) Coarse-grained distribution of element values ln(1 i )a ij times 1 in the reconstructed vector X of all nodes in a Newman-Watts small-world network (NW), where bin accumulation is implemented for the original distribution. The peak centered at zero corresponds to null connections and the other corresponds to actual links. A cut-off value can be set within the gap between the two peaks. (b) The values of ln(1 i )a ij times -1 in the reconstructed vector X for Erdos-Renyi random networks (ER), Watts-Strogatz small-world networks (WS), NW and Barabasi-Albert scale-free networks (BA), where green and yellow points correspond to existent links and null connections, respectively. All the networks share the same cut-off value of about 0.13. (c) Coarse-grained distribution of the structural variance in the NW network, where bin accumulation is exercised on the original distribution. The largest peak centered close zero corresponds to nodes of indirect neighbors of the hidden source and the large positive values of are associated with the immediate neighbors of the hidden source. A cut-off can be set within the largest gap. (d) The variance * i of element values in the reconstructed vector x of all nodes in the presence of a hidden source for ER, WS, NW and BA networks, where green and yellow points correspond to immediate neighbors and indirect neighbors of the hidden source, respectively. All the networks share the same cut-off values of about * i = 0.9. The network size in a,b is 200 and in c,d is 50, = 0.25, = 0.45, i and i are uniformly distributed in the ranges (0.2, 0.4) and (0.4, 0.6), respectively. SIS dynamics are used for the illustration. Panels a and c correspond to Fig. 2a with nt = 0.4 and Fig. 5 in the main text, respectively.
[EX A]:
Supplementary Figures