Given scientific fact as input, generate the question from this fact such that it can be answered from the input.

Insulin is made up of two polypeptide chains.
Insulin is made up of how many polypeptide chains?

Antifreeze lowers it the freezing temperature of the water in car radiators.
What does antifreeze do to the freezing temperature of the water in car radiators?

Sex-linked traits are located on genes on sex chromosomes.
Sex-linked traits are located on genes on what chromosomes?