Q: When synthesizing new DNA, DNA polymerase can add free nucleotides only to the 3' end of the newly forming strand. This results in elongation of the newly forming strand in a 5'--3' direction. No known DNA polymerase is able to begin a new chain (de novo); it can only add a nucleotide onto a pre-existing 3'-OH group, and therefore needs a primer at which it can add the first nucleotide. Primers consist of RNA or DNA bases (or both). In DNA replication, the first two bases are always RNA, and are synthesized by another enzyme called primase. Helicase and topoisomerase II are required to unwind DNA from a double-strand structure to a single-strand structure to facilitate replication of each strand consistent with the semiconservative model of DNA replication.
Can we conclude that can a dna polymerase initiate new strand of nucleic acid independently?
OPTIONS:
- no
- yes
A: no

Q: A drinking establishment is a business whose primary function is the serving of alcoholic beverages for consumption on the premises. Some establishments may also serve food, or have entertainment, but their main purpose is to serve alcoholic beverages. There are different types of drinking establishment ranging from seedy bars or nightclubs, sometimes termed ''dive bars'', to 5,000 seat beer halls and elegant places of entertainment for the elite. A public house, informally known as a ''pub'', is an establishment licensed to serve alcoholic drinks for consumption on the premises in countries and regions of British influence. Although the terms are increasingly used to refer to the same thing, there is a difference between pubs, bars, inns, taverns and lounges where alcohol is served commercially. A tavern or pot-house is, loosely, a place of business where people gather to drink alcoholic beverages and, more than likely, also be served food, though not licensed to put up guests. The word derives from the Latin taberna and the Greek /taverna.
Can we conclude that is there a difference between a pub and a bar?
OPTIONS:
- no
- yes
A: yes

Q: Additionally, Toby Jones, Maximiliano Hernandez, and Garry Shandling reprise their roles from previous MCU films as Arnim Zola, Jasper Sitwell, and Senator Stern, respectively. Georges St-Pierre plays Georges Batroc, a mercenary and a master of the French form of kickboxing known as savate. Callan Mulvey plays Jack Rollins, a member of S.H.I.E.L.D.'s S.T.R.I.K.E. unit. Chin Han, Jenny Agutter (who previously appeared in The Avengers), Alan Dale, and Bernard White appear as members of the World Security Council. Comedic actors Danny Pudi and DC Pierson have small roles as a S.H.I.E.L.D. technician and an Apple Store employee, respectively. Gary Sinise narrates a Captain America-themed Smithsonian Institution exhibit, and Stan Lee cameos as a security guard there. Winter Soldier creator Ed Brubaker makes a cameo as a scientist working on the Winter Soldier. Co-director Joe Russo cameos as a doctor, and Christopher Markus and Stephen McFeely cameo as two S.H.I.E.L.D. interrogators. Thomas Kretschmann, Henry Goodman, Elizabeth Olsen and Aaron Taylor-Johnson appear, uncredited, as Baron Wolfgang von Strucker, Dr. List, the Scarlet Witch, and Quicksilver, respectively, in the mid-credits scene.
Can we conclude that was one of the russo brothers in winter soldier?
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A: yes

Q: Freddy Rodriguez, who portrays hospital administrator Michael Ragosa, revealed on Twitter that he would not be returning for the third season of The Night Shift, as he is starring in a new CBS drama pilot Bull. Jeananne Goossen who portrayed Krista Bell-Heart, is also not returning for the third season; she says that she split ''amicably'' from the show. On March 21, 2016, it was revealed that 90210 alum AnnaLynne McCord had been cast as a recurring character named Jessica Sanders, ''a striking and driven pharamaceutical rep who's always looking for a way to make a deal.'' She will first appear in the fifth episode of the season, and in at least 3 additional episodes. Lindsey Morgan will appear in a guest role as Kryztal, ''a high-strung bride whose injury in a wedding-day accident reveals a deeper and more complicated medical problem.''
Can we conclude that is krista in season 3 of the night shift?
OPTIONS:
- no
- yes
A:
no