In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

Let me give you an example: The severe acute respiratory syndrome (SARS) epidemic originating from China in 2002 was caused by a previously uncharacterized coronavirus that could be identified by specific RT-PCR amplification. Efforts to control future SARS outbreaks depend on the accurate and early identification of SARS-CoV infected patients. A real-time fluorogenic RT-PCR assay based on the 3 -noncoding region (3 -NCR) of SARS-CoV genome was developed as a quantitative SARS diagnostic tool. The ideal amplification efficiency of a sensitive SARS-CoV RT-PCR assay should yield an E value (PCR product concentration increase per amplification cycle) equal to 2.0. It was demonstrated that the 3 -NCR SARS-CoV based RT-PCR reactions could be formulated to reach excellent E values of 1.81, or 91% amplification efficacy. The SARS-CoV cDNA preparations derived from viral RNA extract and the cloned recombinant plasmid both exhibit the identical amplification characteristics, i.e. amplification efficacy using the same PCR formulation developed in this study. The viral genomic copy (or genomic equivalences, GE) per infectious unit (GE/pfu) of SARS-CoV used in this study was also established to be approximate 1200-1600:1. The assay's detection sensitivity could reach 0.005 pfu or 6-8 GE per assay. It was preliminarily demonstrated that the assay could efficiently detect SARS-CoV from clinical specimens of SARS probable and suspected patients identified in Taiwan. The 3 -NCR based SARS-CoV assay demonstrated 100% diagnostic specificity testing samples of patients with acute respiratory disease from a non-SARS epidemic region.
The answer to this example can be: Development and evaluation of an efficient 3 -noncoding region-based SARS coronavirus (SARS-CoV) RT-PCR assay for detection of SARS-CoV
Here is why: It's a correct title because the paragraph described the development of 3 -noncoding region of SARS-CoV genome and assay efficiently detecting SARS-CoV from clinical specimens.

OK. solve this:
Since the 1980s the epidemiology of Clostridium difficile infection (CDI) has been investigated by the application of many different typing or fingerprinting methods. To study the epidemiology of CDI, a typing method with a high discriminatory power, typeability, stability, power, reproducibility and epidemiological concordance is required. It should also have technical advantages, such as ease of performance, relative low cost, and high throughput. A growing number of molecular methods have been applied to C. difficile. For the early and rapid detection of outbreak situations, methods such as restriction enzyme analysis, arbitrary primed polymerase chain reaction (PCR), pulsed-field gel electrophoresis, and PCR ribotyping are commonly used. For long-term epidemiology, multilocus sequence typing, multilocus variable number of tandem repeats analysis (MLVA), and amplified fragment length polymorphism are of interest. Currently, the PCR-ribotyping method and the library of PCR ribotypes in Cardiff are the benchmarks to which most typing studies around the world are compared. Conventional agarose gel-based PCR ribotyping is easy to use and relatively cheap, but analysis of fragment lengths is hampered by poor resolution. Recently, a capillary gel electrophoresisbased PCR ribotyping assay has been developed that significantly reduces the hands-on time required for C. difficile PCR ribotyping. The results were highly reproducible, independent of reagent batches or brands used and allows inter-laboratory comparisons of typing results. The analysis of the sequenced C. difficile genome revealed a high percentage of DNA that consisted of a variable number of tandem repeats (VNTR). Recently, a new MLVA method was developed using smal short tandem repeats (29 bp) to facilitate automated fragment analysis with multicoloured capillary electrophoresis instead of sequencing. In a study using isolates from laboratories in Canada, the Netherlands, the United Kingdom, and the United States, seven C. difficile typing techniques were compared, but only REA and MLVA showed sufficient discrimination to distinguish strains from different outbreaks. MLVA has also been applied to study local outbreaks of C. difficile PCR ribotype 027 strains. MLVA is currently the most discriminative typing method and will contribute significantly to our understanding of the epidemiology of C. difficile. S3 New treatment aspects of CDI The need for new therapeutic alternatives in CDI is clear. Current recurrence rates are unacceptably high. The optimal treatment of severe CDI, infection caused by strains associated with poor outcome (e.g. C. difficile ribotype 027), and multiple recurrences is unclear. There are several potentially promising new CDI treatment approaches under investigation, including antibiotic, anti-toxin and bacterial interference options. Probiotic therapy remains of unproven benefit. As new options become available, prognostic data will be needed to guide on the most approriate therapeutic choices.
Answer:
Oral presentations New aspects of emerging Clostridium difficile infections (CDI) S2 Pros and cons of molecular fingerprinting for Clostridium difficile infections