The Maya developed their first civilization in the Preclassic period. Scholars continue to discuss when this era of Maya civilization began. Maya occupation at Cuello (modern-day Belize) has been carbon dated to around 2600 BC. Settlements were established around 1800 BC in the Soconusco region of the Pacific coast, and the Maya were already cultivating the staple crops of maize, beans, squash, and chili pepper. This period was characterized by sedentary communities and the introduction of pottery and fired clay figurines. During the Middle Preclassic Period, small villages began to grow to form cities. Nakbe in the Peten department of Guatemala is the earliest well-documented city in the Maya lowlands, where large structures have been dated to around 750 BC. The northern lowlands of Yucatan were widely settled by the Middle Preclassic. By approximately 400 BC, early Maya rulers were raising stelae. A developed script was already being used in Peten by the 3rd century BC. In the Late Preclassic Period, the enormous city of El Mirador grew to cover approximately 16 square kilometres (6.2 sq mi). Although not as large, Tikal was already a significant city by around 350 BC. In the highlands, Kaminaljuyu emerged as a principal centre in the Late Preclassic. Takalik Abaj and Chocola were two of the most important cities on the Pacific coastal plain, and Komchen grew to become an important site in northern Yucatan. The Late Preclassic cultural florescence collapsed in the 1st century AD and many of the great Maya cities of the epoch were abandoned; the cause of this collapse is unknown.

Answer this question "Was there a population of these cities?" by extracting the answer from the text above.
The northern lowlands of Yucatan were widely settled by the Middle Preclassic.