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Melatonin is absent from the system or undetectably low during daytime. Its onset in dim light, dim-light melatonin onset (DLMO), at roughly 21:00 (9 p.m.) can be measured in the blood or the saliva. Its major metabolite can also be measured in morning urine. Both DLMO and the midpoint (in time) of the presence of the hormone in the blood or saliva have been used as circadian markers. However, newer research indicates that the melatonin offset may be the more reliable marker. Benloucif et al. found that melatonin phase markers were more stable and more highly correlated with the timing of sleep than the core temperature minimum. They found that both sleep offset and melatonin offset are more strongly correlated with phase markers than the onset of sleep. In addition, the declining phase of the melatonin levels is more reliable and stable than the termination of melatonin synthesis.
When is the melatonin onset?
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The answer: 9 p.m

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The drunkenness and lawlessness created by gin was seen to lead to ruination and degradation of the working classes. The distinction[clarification needed] was illustrated by William Hogarth in his engravings Beer Street and Gin Lane. The Gin Act 1736 imposed high taxes on retailers and led to riots in the streets. The prohibitive duty was gradually reduced and finally abolished in 1742. The Gin Act 1751 however was more successful. It forced distillers to sell only to licensed retailers and brought gin shops under the jurisdiction of local magistrates.
What law placed gin shops under the control of local magistrates?
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The answer: The Gin Act 1751

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The Cineteca Nacional (the Mexican Film Library), near the Coyoacan suburb, shows a variety of films, and stages many film festivals, including the annual International Showcase, and many smaller ones ranging from Scandinavian and Uruguayan cinema, to Jewish and LGBT-themed films. Cinepolis and Cinemex, the two biggest film business chains, also have several film festivals throughout the year, with both national and international movies. Mexico City tops the world in number of IMAX theatres,[citation needed] providing residents and visitors access to films ranging from documentaries to popular blockbusters on these especially large, dramatic screens.
What large movie festival is hosted at the Mexican Film Library?
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The answer: International Showcase

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After gaining the Master Sword, Link is cleansed of the magic that kept him in wolf form, obtaining the Shadow Crystal. Now able to use it to switch between both forms at will, Link is led by Midna to the Mirror of Twilight located deep within the Gerudo Desert, the only known gateway between the Twilight Realm and Hyrule. However, they discover that the mirror is broken. The Sages there explain that Zant tried to destroy it, but he was only able to shatter it into fragments; only the true ruler of the Twili can completely destroy the Mirror of Twilight. They also reveal that they used it a century ago to banish Ganondorf, the Gerudo leader who attempted to steal the Triforce, to the Twilight Realm when executing him failed. Assisted by an underground resistance group they meet in Castle Town, Link and Midna set out to retrieve the missing shards of the Mirror, defeating those they infected. Once the portal has been restored, Midna is revealed to be the true ruler of the Twilight Realm, usurped by Zant when he cursed her into her current form. Confronting Zant, Link and Midna learn that Zant's coup was made possible when he forged a pact with Ganondorf, who asked for Zant's assistance in conquering Hyrule. After Link defeats Zant, Midna recovers the Fused Shadows, but destroys Zant after learning that only Ganondorf's death can release her from her curse. Returning to Hyrule, Link and Midna find Ganondorf in Hyrule Castle, with a lifeless Zelda suspended above his head. Ganondorf fights Link by possessing Zelda's body and eventually by transforming into a beast, but Link defeats him and Midna is able to resurrect Zelda.
What does Link obtain that gives him control over his transformations?
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The answer:
Shadow Crystal