In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

Q: Ubiquitination, a post-translational modification, mediates diverse cellular functions including endocytic transport of molecules. Kaposi's sarcoma-associated herpesvirus (KSHV), an enveloped herpesvirus, enters endothelial cells primarily through clathrin-mediated endocytosis. Whether ubiquitination and proteasome activity regulates KSHV entry and endocytosis remains unknown. We showed that inhibition of proteasome activity reduced KSHV entry into endothelial cells and intracellular trafficking to nuclei, thus preventing KSHV infection of the cells. Three-dimensional (3-D) analyses revealed accumulation of KSHV particles in a cytoplasmic compartment identified as EEA1+ endosomal vesicles upon proteasome inhibition. KSHV particles are colocalized with ubiquitin-binding proteins epsin and eps15. Furthermore, ubiquitination mediates internalization of both KSHV and one of its receptors integrin b1. KSHV particles are colocalized with activated forms of the E3 ligase c-Cbl. Knock-down of c-Cbl or inhibition of its phosphorylation reduced viral entry and intracellular trafficking, resulting in decreased KSHV infectivity. These results demonstrate that ubiquitination mediates internalization of both KSHV and one of its cognate receptors integrin b1, and identify c-Cbl as a potential E3 ligase that facilitates this process.

A: Associated Herpesvirus in Endothelial Cells
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Q: A reversed passive hemagglutination (I~PHA) method was developed for the detection of bovine eoronavirus in fecal specimens. Sheep erythrocytes fixed with glutaraldehyde, and then treated with tannic acid were coated with anti-bovine coronavirus rabbit antibodies purified by affinity chromatography using bovine eoronavirus linked to Sepharose 4B. The RPHA test was carried out by a microtiter method. Erythrocytes coated with purified specific antibodies were agglutinated by bovine coronavirus, but not by bovine rotavirus or enterovirus. The reaction was inhibited by antiserum to bovine coronavirus, confirming the specificity of the reaction. The I~PIIA test detected bovine coronavirus in 13 of 22 fecal specimens (59 per cent), from natural cases of diarrhea, while the positive rates were only 14 per cent (3/22) and 22 per cent (5/22) for immunofluorescent staining of primary cultures of calf kidney cells infected with the specimens, and immune electron microscopy respectively. The advantages of the I~PHA method are its simplicity, high sensitivity and rapidity.

A: Archives of Virology Detection of Bovine Coronavirus in Feces by Reversed Passive Hemagglutination
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Q: Temperature-sensitive mutants of the munne coronavirus JHM induced a sub~ acute demyelinatin 8 encephalomyelitis (SD~E) in youn~ rats. Neurological symptol~,; were associated with marked lesion3 of primary d~my;lination ".m the white matter of the central nervous system (CNS), and developing after an incubation time of several weeks to months. Many rats survived this infection and rco3vered completely from this CNS disease. Amon 8 43 survivors of SDE, 9 ra'~s developed a ~'elapse 27-153 days after onset of the first attack. Neuropathologica.l ex~n~ation ol~ these animals revealed areas of fresh| ~emyelination together with old remyelinated lesions. V'md antisens were detectable in the neighbourhood of fresh les:ions and in some ca~ infectious virus was re-i~lated from rats revealing low antibl~y titers to JHM virus. These results demonstrate that mutants of JHM virus can induca~ a relapsing demyelinating disease process, associated with a persistent in fection, which some similarities to chronic experimental allergic enceph~dor~ayel/tis.

A:
Relapsing Subacute Demyelina~:ing Encephalomyelitis in Rats during the Course of Coronavirus JHM Infection
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