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On the other hand, I2a1b1 (P41.2) is typical of the South Slavic populations, being highest in Bosnia-Herzegovina (>50%). Haplogroup I2a2 is also commonly found in north-eastern Italians. There is also a high concentration of I2a2a in the Moldavian region of Romania, Moldova and western Ukraine. According to original studies, Hg I2a2 was believed to have arisen in the west Balkans sometime after the LGM, subsequently spreading from the Balkans through Central Russian Plain. Recently, Ken Nordtvedt has split I2a2 into two clades N (northern) and S (southern), in relation where they arose compared to Danube river. He proposes that N is slightly older than S. He recalculated the age of I2a2 to be ~ 2550 years and proposed that the current distribution is explained by a Slavic expansion from the area north-east of the Carpathians.

Who has split I2a2 into two clades?
Answer: Ken Nordtvedt
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According to East Asian and Tibetan Buddhism, there is an intermediate state (Tibetan "bardo") between one life and the next. The orthodox Theravada position rejects this; however there are passages in the Samyutta Nikaya of the Pali Canon that seem to lend support to the idea that the Buddha taught of an intermediate stage between one life and the next.[page needed]

There is an intermediate state between one life and the next according to what Buddism?
Answer: East Asian and Tibetan
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In the absence of suitable plate culture techniques, some microbes require culture within live animals. Bacteria such as Mycobacterium leprae and Treponema pallidum can be grown in animals, although serological and microscopic techniques make the use of live animals unnecessary. Viruses are also usually identified using alternatives to growth in culture or animals. Some viruses may be grown in embryonated eggs. Another useful identification method is Xenodiagnosis, or the use of a vector to support the growth of an infectious agent. Chagas disease is the most significant example, because it is difficult to directly demonstrate the presence of the causative agent, Trypanosoma cruzi in a patient, which therefore makes it difficult to definitively make a diagnosis. In this case, xenodiagnosis involves the use of the vector of the Chagas agent T. cruzi, an uninfected triatomine bug, which takes a blood meal from a person suspected of having been infected. The bug is later inspected for growth of T. cruzi within its gut.

What type of eggs may some viruses be grown in?
Answer:
embryonated