Article: The central oscillator generates a self-sustaining rhythm and is driven by two interacting feedback loops that are active at different times of day. The morning loop consists of CCA1 (Circadian and Clock-Associated 1) and LHY (Late Elongated Hypocotyl), which encode closely related MYB transcription factors that regulate circadian rhythms in Arabidopsis, as well as PRR 7 and 9 (Pseudo-Response Regulators.) The evening loop consists of GI (Gigantea) and ELF4, both involved in regulation of flowering time genes. When CCA1 and LHY are overexpressed (under constant light or dark conditions), plants become arrhythmic, and mRNA signals reduce, contributing to a negative feedback loop. Gene expression of CCA1 and LHY oscillates and peaks in the early morning, whereas TOC1 gene expression oscillates and peaks in the early evening. While it was previously hypothesised that these three genes model a negative feedback loop in which over-expressed CCA1 and LHY repress TOC1 and over-expressed TOC1 is a positive regulator of CCA1 and LHY, it was shown in 2012 by Andrew Millar and others that TOC1 in fact serves as a repressor not only of CCA1, LHY, and PRR7 and 9 in the morning loop but also of GI and ELF4 in the evening loop. This finding and further computational modeling of TOC1 gene functions and interactions suggest a reframing of the plant circadian clock as a triple negative-component repressilator model rather than the positive/negative-element feedback loop characterizing the clock in mammals.

Question: Which loop that consists of GI and ELF4 functions?
Ans: evening

Article: A mandolin (Italian: mandolino pronounced [mandolino]; literally "small mandola") is a musical instrument in the lute family and is usually plucked with a plectrum or "pick". It commonly has four courses of doubled metal strings tuned in unison (8 strings), although five (10 strings) and six (12 strings) course versions also exist. The courses are normally tuned in a succession of perfect fifths. It is the soprano member of a family that includes the mandola, octave mandolin, mandocello and mandobass.

Question: What country did the mandolin originate from?
Ans: Italian

Article: Arizona, south of the Gila River was legally bought from Mexico in the Gadsden Purchase on June 8, 1854. Tucson became a part of the United States of America, although the American military did not formally take over control until March 1856. In 1857 Tucson became a stage station on the San Antonio-San Diego Mail Line and in 1858 became 3rd division headquarters of the Butterfield Overland Mail until the line shut down in March 1861. The Overland Mail Corporation attempted to continue running, however following the Bascom Affair, devastating Apache attacks on the stations and coaches ended operations in August 1861.[citation needed]

Question: Where did the US buy land from in the Gadsden Purchase?
Ans: Mexico

Article: LeToya Luckett and Roberson became unhappy with Mathew's managing of the band and eventually were replaced by Farrah Franklin and Michelle Williams. Beyonce experienced depression following the split with Luckett and Roberson after being publicly blamed by the media, critics, and blogs for its cause. Her long-standing boyfriend left her at this time. The depression was so severe it lasted for a couple of years, during which she occasionally kept herself in her bedroom for days and refused to eat anything. Beyonce stated that she struggled to speak about her depression because Destiny's Child had just won their first Grammy Award and she feared no one would take her seriously. Beyonce would later speak of her mother as the person who helped her fight it. Franklin was dismissed, leaving just Beyonce, Rowland, and Williams.

Question: How long was Beyonce depressed?
Ans:
a couple of years