Nguyen was born in Singapore, where her family were Vietnamese boat people, who arrived from Vietnam after the Vietnam War. Nguyen has an older brother, Daniel, and older sister, Terri. When she was one year old, the family relocated to a neighborhood in Houston, Texas and were eventually admitted to a gated community run by a strict Buddhist temple. The family left the community when Nguyen was eight.

During the first season of Fuse TV's dance show, Pants-Off Dance-Off, on which a group of contestants strip to music videos, Nguyen was the most frequent host. In April 2006, during the taping of an interview with MTV's Total Request Live VJs, will.i.am announced that Nguyen had been signed to the Will.I.Am music group, a record label under A&M Records. Despite this major-label signing, Nguyen independently released her first single "I Love U" through iTunes on February 27, 2007, justifying the independent release through her desire to become famous by herself. She also shot a music video for the song. In March 2007, Washington-based record label The Saturday Team released an EP called Sex, by Tila Tequila. On July 27, 2007, Italian website MusicBlob reported that The Saturday Team and distributor Icon Music Entertainment Services sued Nguyen over breaching her contract related to the album. However, Nguyen claimed in a MySpace bulletin that the EP was not authorized for release by her, and was removed from most retailers. The Saturday Team won a legal case, making Sex available for digital purchase. Nguyen made an appearance as one of the 12 strangers in the first game on April 6, 2007 episode of NBC's game show Identity. On March 4, 2007, she made a cameo appearance on the show "The War At Home." She also appeared as a Hooters Girl in the 2007 film I Now Pronounce You Chuck and Larry. In May 2007, Nguyen began filming for a reality show which first aired on MTV on October 9, 2007, as A Shot at Love with Tila Tequila. The program was a bisexual-themed dating show where 16 straight men and 16 lesbian women competed for Nguyen's affections, the twist being that the contestants were not aware of her bisexuality until the end of the first episode. The ten-episode series was produced by SallyAnn Salsano and MTV. The show led to a heated online debate between Nguyen and conservative Christians after an article appeared on The Christian Post on September 13, 2007. After seeing the article, Nguyen wrote an impassioned response in her blog on September 28, 2007, criticizing churches for "bashing" the gay community while thanking God for saving her life. The show premiered for a second season in April 2008 and became a popular gossip subject in Asian media, such as AsianWeek. The season finale premiered July 8, 2008, the winner being Kristy Morgan who declined her "shot at love". On October 9, 2007, Nguyen released her second official single, "Stripper Friends". A video premiered via Yahoo! Music on February 26, 2008 and was released to iTunes on March 4, 2008. The single failed to chart. In April 2008, the single "Paralyze" and its accompanying music video were released via Yahoo! Music and iTunes. The "I Love U Remixes" EP was released to digital music retailers on April 7, 2009. On December 2, 2008, Nguyen released a self-help book, Hooking Up with Tila Tequila: A Guide to Love, Fame, Happiness, Success, and Being the Life of the Party (ISBN 9781439101537).

did she produce any songs?
Nguyen independently released her first single "I Love U" through iTunes