Detailed Instructions: Given a passage classify if the passage has a definite objective/aim/goal or not. Output '1' if the passage has a defininte objective/aim/goal and output '0' if the passage does not have a definite objective/aim/goal.
Q: In a previous study, we showed that IFN- and TNF- induce bone marrow mesenchymal stem cell (BMMSC) apoptosis and absorption of tissue-engineered cartilage, but the induced regulatory T cells (iTreg) inhibit the function of IFN- and TNF-. In this study, we investigated the effect of iTreg cells on the absorption of tissue-engineered cartilage caused by endogenous IFN- and TNF-.', 'We transfected the TGF-1 gene into BMMSCs co-cultured with CD4(+) T lymphocytes. Then, we assessed the expression of iTreg cell markers (Foxp3, CD25 and CD 39) and IFN- and TNF- and the level of apoptosis of BMMSCs. In addition, we characterized chondrogenic-committed cells from TGF-1(+)BMMSCs and explored the role of iTreg cells.', 'IFN- and TNF- were detected in the groups with CD4(+) T cells. In the group in which TGF-1(+)BMMSCs were co-cultured with CD4(+) T cells, we observed Foxp3(+)Treg/ CD25(+)CD39(+) (17.58 0.45%) cells as well as significant inhibition of BMMSC apoptosis and tissue-engineered cartilage absorption.
A:
0