TASK DEFINITION: In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.
PROBLEM: The massive abundance of studies relating to tropical medicine and health has increased strikingly over the last few decades. In the field of tropical medicine and health, a well-conducted systematic review and meta-analysis (SR/MA) is considered a feasible solution for keeping clinicians abreast of current evidence-based medicine. Understanding of SR/MA steps is of paramount importance for its conduction. It is not easy to be done as there are obstacles that could face the researcher. To solve those hindrances, this methodology study aimed to provide a step-by-step approach mainly for beginners and junior researchers, in the field of tropical medicine and other health care fields, on how to properly conduct a SR/MA, in which all the steps here depicts our experience and expertise combined with the already well-known and accepted international guidance. We suggest that all steps of SR/MA should be done independently by 2-3 reviewers' discussion, to ensure data quality and accuracy. Conclusion: SR/MA steps include the development of research question, forming criteria, search strategy, searching databases, protocol registration, title, abstract, full-text screening, manual searching, extracting data, quality assessment, data checking, statistical analysis, double data checking, and manuscript writing.

SOLUTION: A step by step guide for conducting a systematic review and meta-analysis with simulation data

PROBLEM: The high mutation rate of the human immunodeficiency virus type 1 (HIV-1) virus is a major problem since it evades the function of antibodies and chemical inhibitors. Here, we demonstrate a viral detection strategy based on synthetic biology principles to detect a specific viral function rather than a particular viral protein. The resistance caused by mutations can be circumvented since the mutations that cause the loss of function also incapacitate the virus. Many pathogens encode proteases that are essential for their replication and that have a defined substrate specificity. A genetically encoded sensor composed of a fused membrane anchor, viral protease target site, and an orthogonal transcriptional activator was engineered into a human cell line. The HIV-1 protease released the transcriptional activator from the membrane, thereby inducing transcription of the selected genes. The device was still strongly activated by clinically relevant protease mutants that are resistant to protease inhibitors. In the future, a similar principle could be applied to detect also other pathogens and functions.

SOLUTION: Function-Based Mutation-Resistant Synthetic Signaling Device Activated by HIV-1 Proteolysis

PROBLEM: During th e pas t thre e decades , ther e ha s bee n a growin g realizatio n tha t breast-feeding no t only provides the nutritional requirements of the infant , but als o supplie s a hos t o f defens e factor s fo r th e protectio n o f th e recipient and/o r th e mammar y gland . Th e stud y o f thi s remarkabl e defense syste m in huma n mil k ha s bee n difficult , however , becaus e o f it s biochemical complexities , th e smal l concentration s o f certai n ver y poten t agents i n huma n milk , th e nee d t o develo p specia l method s t o quantif y certain factor s becaus e o f thei r particula r form s i n huma n milk , th e compartmentalization o f some of the agents, and the dynamic effects o f th e length o f lactatio n an d othe r materna l factor s upo n th e concentration s o r functions o f th e component s o f th e system . I n thi s chapter, w e summariz e the current information concernin g the molecular forms, concentrations i n milk durin g th e severa l phase s o f lactation , biologica l activities , fat e i n th e recipient infant, an d in vivo functions o f the defense agent s in human milk . (2 ) mos t component s of th e immunologi c syste m i n huma n mil k ar e produce d throughou t lactation an d durin g gradua l weaning ; (3 ) th e factor s ar e usuall y commo n to othe r mucosa l sites ; (4 ) the y ar e adapte d t o resis t digestio n i n th e gastrointestinal trac t of th e recipien t infant ; (5 ) they protec t b y noninflammatory mechanisms ; an d (6 ) th e agent s ac t synergisticall y wit h eac h othe r or with defense agent s produced b y the recipient. Representativ e example s of solubl e defens e agent s ar e liste d i n Tabl e I .

SOLUTION:
Defense Agents in Milk A. Defens e Agent s i n Huma n Mil k A. Factors with Nonnutrltive Functions In Human Milk The defens e agent s i n huma n milk , thoug h biochemicall y diverse , shar e certain features : (1 ) ther e i s ofte n a n invers e relationshi p betwee n th e 728 Armon