TASK DEFINITION: In this task, you're given a paragraph and title from the research paper. Your task is to classify whether the given title is suitable or not for the research paper based on the given paragraph. Return "True" if title is proper according to paragraph else "False".
PROBLEM: Paragraph: Interactions between the immune system and the central nervous system (CNS) constitute the most complex and interactive regulatory network in mammals. The high degree of specialization of cell types that comprise the CNS, and their intricate communication, controls both cognitive and vital functions. Disruption of the communication network and poor CNS regenerative properties make this organ vulnerable to microbial as well as physical injury. Although it is known that host responses must be strictly regulated to preserve CNS function and to minimize the incidence of autoimmunity, the factors regulating CNS immune and repair responses are not well understood. In addition to the absence of a dedicated lymphatic drainage system, CNS cells express few, if any, molecules encoded by the major histocompatibility complex (MHC) 1-3 . Therefore, in the quiescent CNS there is little endogenous antigen presentation or potential to activate T cells. Although the underlying basis for this limited immunological activity is not completely understood, interactions between both neurons and the glial population represented by microglia, astrocytes and oligodendrocytes (BOX 1), as well as constitutive secretion of neurotrophins and transforming growth factor-, might contribute to this quiescent resting state 1,4-7 . The limited expression of adhesion molecules by endothelial cells of the blood-brain barrier (BBB) and the presence of tight junctions between these cells also limit or prevent large molecules, such as antibodies, and T cells from entering the CNS 2,8 . Despite this, a few activated/memory CD4 + and CD8 + T cells randomly patrol the CNS in the absence of 'danger' signals, and either exit or die in situ in the absence of antigen recognition 2,8 . Abstract | Several viruses infect the mammalian central nervous system (CNS), some with devastating consequences, others resulting in chronic or persistent infections associated with little or no overt pathology. Coronavirus infection of the murine CNS illustrates the contributions of both the innate immune response and specific host effector mechanisms that control virus replication in distinct CNS cell types. Despite T-cell-mediated control of acute virus infection, host regulatory mechanisms, probably designed to protect CNS integrity, contribute to the failure to eliminate virus. Distinct from cytolytic effector mechanisms expressed during acute infection, non-lytic humoral immunity prevails in suppressing infectious virus during persistence.
Title: Neurotrophins Coronavirus infection of the central nervous system: host-virus stand-off

SOLUTION: False

PROBLEM: Paragraph: The mucus-dwelling parasite Ostertagia ostertagi is one of the most important gastrointestinal nematodes in cattle. Our group has previously demonstrated the protective capacity of a vaccine against this parasite based on a native activation-associated secreted protein ASP1 (nASP) in combination with the saponin adjuvant QuilA. The aim of the current study was to analyse the effect of both antigen and adjuvant on the cellular and humoral vaccine-induced immune responses by comparing the native ASP to a recombinant version expressed in Pichia pastoris (pASP) and replacing QuilA by Al(OH) 3 . Immunization of cattle with the protective nASP+QuilA vaccine was associated with antigen-induced proliferation of natural killer (NK) cells combined with IFN- secretion and the induction of a mixed IgG1/IgG2 antibody response. ASP-specific activation and proliferation of NK cells was also observed in mice following the same vaccination regime. Replacing QuilA by Al(OH) 3 or nASP by pASP significantly decreased the capacity of the vaccines to trigger both NK cell activation and antibody responses and failed to induce protection against a challenge infection. Reduction of the structurally anchoring disulphide bonds of the nASP completely abolished its ability to induce NK cell activation and antibody responses, highlighting the importance of protein conformation for the immunostimulatory activity.
Title: Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response

SOLUTION: True

PROBLEM: Paragraph: Apart from the core (21 kD), a novel hepatitis C virus (HCV) frame shift protein (F1) is synthesized from the initiation codon of the polyprotein sequence followed by ribosomal frame shift into the A2/+1 reading frame. To date, no information is available on F1 protein of Indian isolates, and hence detection of antibodies for F1 protein in Indian patients assumes great relevance. Specific primers have been designed to amplify sequence coding for 120aa of truncated F1 (tF1). The amplified tF1 has been cloned in bacterial expression vector, pET21b for expression in Escherichia coli. Partially purified expressed protein has been subjected to western blot analysis using patients' sera. Three HCV positive sera employed in western analysis showed positive signals to tF1, while sera from uninfected individuals failed to give any signals. Further, results of western blots, carried out with patients sera titrated with purified core protein, confirmed the presence of antibodies specific to F1. The positive signal observed for F1 in western analysis with HCV infected sera suggests that F1 protein is synthesized in the natural course of HCV infection in Indian patients as well. Presence of antibodies against F1 protein of subtype 1c has been demonstrated, for the first time, in Indian patients. #
Title: Expression of alternate reading frame protein (F1) of hepatitis C virus in Escherichia coli and detection of antibodies for F1 in Indian patients

SOLUTION:
True