Question: As with bacterial classification, identification of bacteria is increasingly using molecular methods. Diagnostics using DNA-based tools, such as polymerase chain reaction, are increasingly popular due to their specificity and speed, compared to culture-based methods. These methods also allow the detection and identification of "viable but nonculturable" cells that are metabolically active but non-dividing. However, even using these improved methods, the total number of bacterial species is not known and cannot even be estimated with any certainty. Following present classification, there are a little less than 9,300 known species of prokaryotes, which includes bacteria and archaea; but attempts to estimate the true number of bacterial diversity have ranged from 107 to 109 total species and even these diverse estimates may be off by many orders of magnitude.
Is there an answer to this question: What tool does DNA based diagnostics use?

Answer: polymerase chain reaction

Question: That said, the score does not provide complete and exact instructions on how to perform a historical work. Even if the tempo is written with an Italian instruction (e.g., Allegro), we do not know exactly how fast the piece should be played. As well, in the Baroque era, many works that were designed for basso continuo accompaniment do not specify which instruments should play the accompaniment or exactly how the chordal instrument (harpsichord, lute, etc.) should play the chords, which are not notated in the part (only a figured bass symbol beneath the bass part is used to guide the chord-playing performer). The performer and/or the conductor have a range of options for musical expression and interpretation of a scored piece, including the phrasing of melodies, the time taken during fermatas (held notes) or pauses, and the use (or choice not to use) of effects such as vibrato or glissando (these effects are possible on various stringed, brass and woodwind instruments and with the human voice).
Is there an answer to this question: The score does not provide complete and exact instruction on how to do what?

Answer: perform a historical work

Question: In the view of many associated with the Radical Reformation, the Magisterial Reformation had not gone far enough. Radical Reformer, Andreas von Bodenstein Karlstadt, for example, referred to the Lutheran theologians at Wittenberg as the "new papists". Since the term "magister" also means "teacher", the Magisterial Reformation is also characterized by an emphasis on the authority of a teacher. This is made evident in the prominence of Luther, Calvin, and Zwingli as leaders of the reform movements in their respective areas of ministry. Because of their authority, they were often criticized by Radical Reformers as being too much like the Roman Popes. A more political side of the Radical Reformation can be seen in the thought and practice of Hans Hut, although typically Anabaptism has been associated with pacifism.
Is there an answer to this question: Which reformation was seen as not being effective enough?

Answer: the Magisterial Reformation

Question: Federalism has a long tradition in German history. The Holy Roman Empire comprised many petty states numbering more than 300 around 1796. The number of territories was greatly reduced during the Napoleonic Wars (17961814). After the Congress of Vienna (1815), 39 states formed the German Confederation. The Confederation was dissolved after the Austro-Prussian War and replaced by a North German Federation under Prussian hegemony; this war left Prussia dominant in Germany, and German nationalism would compel the remaining independent states to ally with Prussia in the Franco-Prussian War of 187071, and then to accede to the crowning of King Wilhelm of Prussia as German Emperor. The new German Empire included 25 states (three of them, Hanseatic cities) and the imperial territory of Alsace-Lorraine. The empire was dominated by Prussia, which controlled 65% of the territory and 62% of the population. After the territorial losses of the Treaty of Versailles, the remaining states continued as republics of a new German federation. These states were gradually de facto abolished and reduced to provinces under the Nazi regime via the Gleichschaltung process, as the states administratively were largely superseded by the Nazi Gau system.
Is there an answer to this question: What was the Holy Roman Empire replaced with?

Answer:
unanswerable