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Transcription produces a single-stranded RNA molecule known as messenger RNA, whose nucleotide sequence is complementary to the DNA from which it was transcribed.:6.1 The mRNA acts as an intermediate between the DNA gene and its final protein product. The gene's DNA is used as a template to generate a complementary mRNA. The mRNA matches the sequence of the gene's DNA coding strand because it is synthesised as the complement of the template strand. Transcription is performed by an enzyme called an RNA polymerase, which reads the template strand in the 3' to 5' direction and synthesizes the RNA from 5' to 3'. To initiate transcription, the polymerase first recognizes and binds a promoter region of the gene. Thus, a major mechanism of gene regulation is the blocking or sequestering the promoter region, either by tight binding by repressor molecules that physically block the polymerase, or by organizing the DNA so that the promoter region is not accessible.:7

How does the nucleotide sequence of mRNA compare to DNA?
Answer: complementary to the DNA from which it was transcribed
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Many parts of the Americas are still populated by indigenous peoples; some countries have sizable populations, especially Belize, Bolivia, Chile, Ecuador, Greenland, Guatemala, Mexico, and Peru. At least a thousand different indigenous languages are spoken in the Americas. Some, such as the Quechuan languages, Aymara, Guarani, Mayan languages, and Nahuatl, count their speakers in millions. Many also maintain aspects of indigenous cultural practices to varying degrees, including religion, social organization, and subsistence practices. Like most cultures, over time, cultures specific to many indigenous peoples have evolved to incorporate traditional aspects, but also cater to modern needs. Some indigenous peoples still live in relative isolation from Western culture and a few are still counted as uncontacted peoples.

What have some indigenous peoples managed to remain in relative isolation from?
Answer: Western culture
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At the close of the 1991 season, a proposal was tabled for the establishment of a new league that would bring more money into the game overall. The Founder Members Agreement, signed on 17 July 1991 by the game's top-flight clubs, established the basic principles for setting up the FA Premier League. The newly formed top division would have commercial independence from The Football Association and the Football League, giving the FA Premier League licence to negotiate its own broadcast and sponsorship agreements. The argument given at the time was that the extra income would allow English clubs to compete with teams across Europe.

What did this agreement establish?
Answer:
established the basic principles for setting up the FA Premier League.