Given the task definition and input, reply with output. Given a passage with a context and an answer, construct a question on the basis of the information present in the passage. Construct the question in such a way that (i) it is unambiguous, (ii) it is answerable from the passage, (iii) its answer is unique (iv) it answer uses text phrases from the passage. Avoid creating questions that (i) can't be answered correctly without actually understanding the passage and (ii) uses the same words or phrases given in the passage.

Context: Periprosthetic osteolysis, involving RANK/RANKL/osteoprotegerin (OPG) and TNF-/NFB signaling, contributes to bone resorption and inflammation. We constructed lentivirus vectors to inhibit TNF- and enhance OPG expression and assessed their impacts on wear debris-induced inflammation and osteoclastogenesis in an osteoclast/osteoblast coculture system.', 'We transduced mouse osteoblastic MC3T3-E1 cells with Lenti-negative control (Lenti-NC), Lenti-OPG or Lenti-siTNF-OPG, and murine macrophage/monocyte RAW264.7 cells with Lenti-NC, Lenti-TNF- siRNA or Lenti-siTNF-OPG. Then, TNF- and OPG protein levels were evaluated by enzyme-linked immunosorbent assay. We cocultured transduced MC3T3-E1 and RAW264.7 cells in transwell chambers in the presence of 0.1 mg/mL Ti particles to investigate the capacity of TNF- inhibition to reduce wear debris-induced inflammation. We also assessed mRNA levels TNF-, IL-1, IL-6 and OPG by RT-PCR as well as osteoclastogenesis by tartrate-resistant acid phosphatase.', 'Lenti-siTNF-OPG ameliorated Ti-particle-induced expression of TNF-, IL-1, IL-6 in MC3T3-E1/RAW264.7 cocultures, while enhancing mRNA and protein levels of OPG, and reducing the fraction of tartrate-resistant acid phosphatase (TRAP)+ cells.
Answer: Lenti-siTNF-OPG can inhibit the wear debris-induced inflammatory responses and osteoclastogenesis in vitro, and may represent a promising therapeutic candidate for the treatment or prevention of wear particle-induced osteolysis.
Does tNF- suppression and osteoprotegerin overexpression inhibit wear debris-induced inflammation and osteoclastogenesis in vitro?